LOCUS       DACTGW010000001        72219 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.001, whole genome shotgun sequence.
ACCESSION   DACTGW010000001 DACTGW010000000
VERSION     DACTGW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 72219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.001"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            175..291
                     /locus_tag="I9573_000001"
     CDS             175..291
                     /locus_tag="I9573_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HAT4794648.1"
                     /translation="MDDDINMIAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            455..1522
                     /locus_tag="I9573_000002"
     CDS             455..1522
                     /locus_tag="I9573_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAT4794649.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PNSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1714..2244
                     /gene="rnmV"
                     /locus_tag="I9573_000003"
     CDS             1714..2244
                     /gene="rnmV"
                     /locus_tag="I9573_000003"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002846650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAT4794650.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMQRIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2415..3284
                     /gene="rsmA"
                     /locus_tag="I9573_000004"
     CDS             2415..3284
                     /gene="rsmA"
                     /locus_tag="I9573_000004"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAT4794651.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3350..4621
                     /locus_tag="I9573_000005"
     CDS             3350..4621
                     /locus_tag="I9573_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794652.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDSDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEEYEELEEDEEIEDNEESDRDEDS
                     EEEYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRV
                     KVQEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGV
                     SQGESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMP
                     YLGYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGST
                     GFLRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4772..5887
                     /locus_tag="I9573_000006"
     CDS             4772..5887
                     /locus_tag="I9573_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAT4794653.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6073..6969
                     /locus_tag="I9573_000007"
     CDS             6073..6969
                     /locus_tag="I9573_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAT4794654.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASIVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7039..7836
                     /locus_tag="I9573_000008"
     CDS             7039..7836
                     /locus_tag="I9573_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794655.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7892..9244)
                     /locus_tag="I9573_000009"
     CDS             complement(7892..9244)
                     /locus_tag="I9573_000009"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAT4794656.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9463..10341
                     /gene="purR"
                     /locus_tag="I9573_000010"
     CDS             9463..10341
                     /gene="purR"
                     /locus_tag="I9573_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAT4794657.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10497..10775
                     /locus_tag="I9573_000011"
     CDS             10497..10775
                     /locus_tag="I9573_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SpoVG"
                     /protein_id="HAT4794658.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            10985..12364
                     /gene="glmU"
                     /locus_tag="I9573_000012"
     CDS             10985..12364
                     /gene="glmU"
                     /locus_tag="I9573_000012"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAT4794659.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEAEVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSNVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SVVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12459..13409
                     /locus_tag="I9573_000013"
     CDS             12459..13409
                     /locus_tag="I9573_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAT4794660.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      14249..14334
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14496..15017
                     /locus_tag="I9573_000014"
     CDS             14496..15017
                     /locus_tag="I9573_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAT4794661.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNDEGVTLTEAQSAKLLSDIGENKIYTSVTADNLGNPLTSNTKVDNKVLY
                     IVLIDNTVMDSTK"
     gene            15168..16844
                     /locus_tag="I9573_000015"
     CDS             15168..16844
                     /locus_tag="I9573_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HAT4794662.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            16856..18064
                     /locus_tag="I9573_000016"
     CDS             16856..18064
                     /locus_tag="I9573_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HAT4794663.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            18079..19791
                     /locus_tag="I9573_000017"
     CDS             18079..19791
                     /locus_tag="I9573_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794664.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETHDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            19784..20692
                     /gene="pilO"
                     /locus_tag="I9573_000018"
     CDS             19784..20692
                     /gene="pilO"
                     /locus_tag="I9573_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HAT4794665.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            20719..21285
                     /locus_tag="I9573_000019"
     CDS             20719..21285
                     /locus_tag="I9573_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAT4794666.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            21322..21849
                     /locus_tag="I9573_000020"
     CDS             21322..21849
                     /locus_tag="I9573_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAT4794667.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            21870..23408
                     /locus_tag="I9573_000021"
     CDS             21870..23408
                     /locus_tag="I9573_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794668.1"
                     /translation="MRKWNKFKSERGAALVLVLIVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEKNIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            23508..24590
                     /locus_tag="I9573_000022"
     CDS             23508..24590
                     /locus_tag="I9573_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAT4794669.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESIFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            24571..25353
                     /locus_tag="I9573_000023"
     CDS             24571..25353
                     /locus_tag="I9573_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAT4794670.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGILFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILIFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            25455..26105
                     /locus_tag="I9573_000024"
     CDS             25455..26105
                     /locus_tag="I9573_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAT4794671.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            26119..26679
                     /locus_tag="I9573_000025"
     CDS             26119..26679
                     /locus_tag="I9573_000025"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAT4794672.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            26696..30082
                     /gene="mfd"
                     /locus_tag="I9573_000026"
     CDS             26696..30082
                     /gene="mfd"
                     /locus_tag="I9573_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAT4794673.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            30113..31108
                     /locus_tag="I9573_000027"
     CDS             30113..31108
                     /locus_tag="I9573_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAT4794674.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            31232..31771
                     /gene="spoVT"
                     /locus_tag="I9573_000028"
     CDS             31232..31771
                     /gene="spoVT"
                     /locus_tag="I9573_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009167752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HAT4794675.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            31891..33504
                     /locus_tag="I9573_000029"
     CDS             31891..33504
                     /locus_tag="I9573_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAT4794676.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            33611..35059
                     /gene="mazG"
                     /locus_tag="I9573_000030"
     CDS             33611..35059
                     /gene="mazG"
                     /locus_tag="I9573_000030"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAT4794677.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            35148..35426
                     /locus_tag="I9573_000031"
     CDS             35148..35426
                     /locus_tag="I9573_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAT4794678.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            35498..35740
                     /locus_tag="I9573_000032"
     CDS             35498..35740
                     /locus_tag="I9573_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAT4794679.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            35802..36056
                     /locus_tag="I9573_000033"
     CDS             35802..36056
                     /locus_tag="I9573_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HAT4794680.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            36057..36590
                     /locus_tag="I9573_000034"
     CDS             36057..36590
                     /locus_tag="I9573_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794681.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            36596..36880
                     /locus_tag="I9573_000035"
     CDS             36596..36880
                     /locus_tag="I9573_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAT4794682.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            36993..37913
                     /locus_tag="I9573_000036"
     CDS             36993..37913
                     /locus_tag="I9573_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HAT4794683.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            38003..40375
                     /gene="spoIIE"
                     /locus_tag="I9573_000037"
     CDS             38003..40375
                     /gene="spoIIE"
                     /locus_tag="I9573_000037"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HAT4794684.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNNEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            40726..42618
                     /gene="pepF"
                     /locus_tag="I9573_000038"
     CDS             40726..42618
                     /gene="pepF"
                     /locus_tag="I9573_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAT4794685.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAIAVMNNN
                     DTEDDAEKKQEVDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMSDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESVDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            42732..43697
                     /gene="galU"
                     /locus_tag="I9573_000039"
     CDS             42732..43697
                     /gene="galU"
                     /locus_tag="I9573_000039"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAT4794686.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            44197..45801
                     /locus_tag="I9573_000040"
     CDS             44197..45801
                     /locus_tag="I9573_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAT4794687.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQENRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            45954..46154
                     /gene="rpmE"
                     /locus_tag="I9573_000041"
     CDS             45954..46154
                     /gene="rpmE"
                     /locus_tag="I9573_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAT4794688.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            46299..47192
                     /locus_tag="I9573_000042"
     CDS             46299..47192
                     /locus_tag="I9573_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HAT4794689.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            47210..48058
                     /gene="prmC"
                     /locus_tag="I9573_000043"
     CDS             47210..48058
                     /gene="prmC"
                     /locus_tag="I9573_000043"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAT4794690.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            48102..49166
                     /gene="prfA"
                     /locus_tag="I9573_000044"
     CDS             48102..49166
                     /gene="prfA"
                     /locus_tag="I9573_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAT4794691.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            49295..49975
                     /locus_tag="I9573_000045"
     CDS             49295..49975
                     /locus_tag="I9573_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAT4794692.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            50004..51044
                     /locus_tag="I9573_000046"
     CDS             50004..51044
                     /locus_tag="I9573_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAT4794693.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            51061..51510
                     /locus_tag="I9573_000047"
     CDS             51061..51510
                     /locus_tag="I9573_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HAT4794694.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            51527..51979
                     /gene="rpiB"
                     /locus_tag="I9573_000048"
     CDS             51527..51979
                     /gene="rpiB"
                     /locus_tag="I9573_000048"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HAT4794695.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            52015..52644
                     /gene="upp"
                     /locus_tag="I9573_000049"
     CDS             52015..52644
                     /gene="upp"
                     /locus_tag="I9573_000049"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAT4794696.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            52944..53759
                     /locus_tag="I9573_000050"
     CDS             52944..53759
                     /locus_tag="I9573_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HAT4794697.1"
                     /translation="MKNCIAVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            53985..54422
                     /locus_tag="I9573_000051"
     CDS             53985..54422
                     /locus_tag="I9573_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HAT4794698.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            54607..54834
                     /locus_tag="I9573_000052"
     CDS             54607..54834
                     /locus_tag="I9573_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HAT4794699.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            54839..55225
                     /locus_tag="I9573_000053"
     CDS             54839..55225
                     /locus_tag="I9573_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HAT4794700.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAIRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            55228..55932
                     /gene="atpB"
                     /locus_tag="I9573_000054"
     CDS             55228..55932
                     /gene="atpB"
                     /locus_tag="I9573_000054"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAT4794701.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            56001..56261
                     /gene="atpE"
                     /locus_tag="I9573_000055"
     CDS             56001..56261
                     /gene="atpE"
                     /locus_tag="I9573_000055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAT4794702.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            56384..56881
                     /gene="atpF"
                     /locus_tag="I9573_000056"
     CDS             56384..56881
                     /gene="atpF"
                     /locus_tag="I9573_000056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAT4794703.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            56878..57423
                     /locus_tag="I9573_000057"
     CDS             56878..57423
                     /locus_tag="I9573_000057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAT4794704.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            57440..58942
                     /locus_tag="I9573_000058"
     CDS             57440..58942
                     /locus_tag="I9573_000058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAT4794705.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            58970..59848
                     /gene="atpG"
                     /locus_tag="I9573_000059"
     CDS             58970..59848
                     /gene="atpG"
                     /locus_tag="I9573_000059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HAT4794706.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            59861..61255
                     /gene="atpD"
                     /locus_tag="I9573_000060"
     CDS             59861..61255
                     /gene="atpD"
                     /locus_tag="I9573_000060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAT4794707.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            61258..61518
                     /gene="atpC"
                     /locus_tag="I9573_000061"
     CDS             61258..61518
                     /gene="atpC"
                     /locus_tag="I9573_000061"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HAT4794708.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            61980..62360
                     /locus_tag="I9573_000062"
     CDS             61980..62360
                     /locus_tag="I9573_000062"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAT4794709.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            62375..62836
                     /locus_tag="I9573_000063"
     CDS             62375..62836
                     /locus_tag="I9573_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAT4794710.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            62857..63447
                     /locus_tag="I9573_000064"
     CDS             62857..63447
                     /locus_tag="I9573_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794711.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            63462..64619
                     /gene="alr"
                     /locus_tag="I9573_000065"
     CDS             63462..64619
                     /gene="alr"
                     /locus_tag="I9573_000065"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAT4794712.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            64652..64939
                     /locus_tag="I9573_000066"
     CDS             64652..64939
                     /locus_tag="I9573_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HAT4794713.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            64908..65291
                     /locus_tag="I9573_000067"
     CDS             64908..65291
                     /locus_tag="I9573_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAT4794714.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            65462..66289
                     /locus_tag="I9573_000068"
     CDS             65462..66289
                     /locus_tag="I9573_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAT4794715.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            66282..67223
                     /locus_tag="I9573_000069"
     CDS             66282..67223
                     /locus_tag="I9573_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HAT4794716.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGINAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            67646..68740
                     /locus_tag="I9573_000070"
     CDS             67646..68740
                     /locus_tag="I9573_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794717.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            68966..69382
                     /locus_tag="I9573_000071"
     CDS             68966..69382
                     /locus_tag="I9573_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794718.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(69564..70133)
                     /locus_tag="I9573_000072"
     CDS             complement(69564..70133)
                     /locus_tag="I9573_000072"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAT4794719.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            70287..71444
                     /locus_tag="I9573_000073"
     CDS             70287..71444
                     /locus_tag="I9573_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HAT4794720.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            71631..>72219
                     /locus_tag="I9573_000074"
     CDS             71631..>72219
                     /locus_tag="I9573_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAT4794721.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYDNKNKNTKNSRNRYRNKNVKSDFGEITLNIPRDRNGDFEPKVIRNYENDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLIAKITDRVLPLAHEWQSRALDM
                     VYPIVFMGAIHYKVRSEGRIINKAAYIAIGVNLEGI"
BASE COUNT        29059 a         7680 c        13429 g        22051 t
ORIGIN      
        1 atatatatat tttaatttga gcagtttgtt atatattata aaaaaagaga aaaataaaag
       61 gtaaattgtt tctggataac ttaaaattgg aagtaagaat atttaactag agaagaatta
      121 tttaagttca ggttagctct aggagtagct gattttatag gagcaataga ttttatggat
      181 gatgatataa acatgatagc aattagacca gaagatataa gggtagaatt taatagagat
      241 ttcaaagaaa ttatagacct caaactcaga ccgtatcgat ataaaaacta aagctagatg
      301 atatagtgtt gtatatagga ccatcagctg ttatggaagt attagtgctt ttaacaaata
      361 taatagtaaa acttttgtat gaattgataa taaaaaaatt aaaaaccaag acataaaaat
      421 ggcaacaaaa agaagttgaa taagggaggt aaaaatgaaa tttaagaagt tagtctcttt
      481 gggaatatta tcaacacttt tattgagtgt aataactgga tgtagtaatg tttcatcagg
      541 ttcaaactta aagtctaata ataagacaaa aggagagtta acagtttata cagcaattga
      601 ggaagatagt atagatacat acttagctac atttaaagag aaatatccag atattaaatt
      661 gaatgtactt agattatcag taggaggtat aacagcaaga ttattagcag aaaaagataa
      721 tcctaaagca gatgtgttat ggggtgttgc agctacaagt ttgataattt tagatgacaa
      781 agaaatgcta gagccatacg ctccgaaagg ctttgataag gtaaatcctg catttaaaga
      841 tgataagaaa gttccttctt gggttggaat agatgcttgg atgactggta taacagtaaa
      901 tactaaagaa ttaaaagcta aaaacatatc agctcctaat tcttatgaag atttaataaa
      961 gccagaatat aaagatctta tatctatgcc tagtccagct tcttcaggaa caggatattt
     1021 aacagtatca gaactcatac aaattatggg tgaagaaaaa gcatggcagt atatggataa
     1081 attgcataaa aatattggtg tatacactca gtctggttca gcgccagcta tattagcagc
     1141 ttcaggagaa tatcccattg gtatatcttt tggaaataga ggtataaagt taaaaggtga
     1201 aggttatcca gtagaagtag tatttccaaa agaaggttct ggatgggaga tagaatcaaa
     1261 tgcacttgtg aaaaaagata atataaaaga agcatcaaaa gtatttttag attgggctat
     1321 aagtaaagat gcaatgaatg agtattctaa gaattttgca gttactacaa ttgacacggg
     1381 aaatccggtg ccagaaggat ttccaaagaa tccattagaa cagatgatag ataatgattt
     1441 aaaatcagca gcaagaaata gagaggctat actaaataaa tggatagaaa aatatgatgg
     1501 aaagacagaa aaagagagct aagccttaca ataaaattta gtacaaaaag cgcctagaat
     1561 ataagtttta catctaggct cttttgatat atttatttaa aatgcatatt tctatataaa
     1621 aataaatagt ttagtacctt gattaaatta aataaaaacc ttaaaataaa gagatgtaaa
     1681 gaaaaactaa aaacgaataa aaggtgaatt tatatgataa aagaaataat agtagtagag
     1741 ggtagagatg acgttacagc agttaagaga gcaatagatg cagaacttat aactacaggt
     1801 ggatttggat ttccaaaagg tgtaatgcag cgtataaaag ctgctaatga aagacgtggt
     1861 gttataatat ttacagaccc agattttgca ggtgaaaaaa taagaaaaaa aatagcatca
     1921 gaagttccag gatgtaaaca tgcatttcta ccaagagagg aagctaaaaa ggatggagat
     1981 ataggtattg agaatgctac tcctcaaagt ataatagcag ctctgaataa agttagaact
     2041 gaaaatacag aaaaaagaga tgaatttaag caagttgact taattagaaa tggtcttata
     2101 ggtaatgaag aagcatcact tagaagagat gcacttggaa aaatacttgg aattggatat
     2161 ggaaatgcaa aacaattttt aaatagattg aataattatg gagttgaaag agaagaattt
     2221 atagatgcat taaaacaaat ctaaacgtaa tgtttaatgt atgaaaaata tgtatataaa
     2281 ataaattgtg gaattacggt taatttaaga taaattattt ataatatata ccttatttta
     2341 aatcaatgta aatacatata aataagtata attaataaaa ttaaatatca aaataagtta
     2401 aaaaggagta aataatggat agactatctt cacataatgc aacaaaggaa gtagtacaaa
     2461 aatataattt taaattttca aagtctttag gtcaaaattt cttgatagat agtaatataa
     2521 ttgataaaat cttatctgga gctagaatca caagaggaga taatatcata gaagtaggac
     2581 ctggtatagg aactcttact cgtgagatgg gtaaaatagc agaaaaagta gtggctatag
     2641 aaatcgatag aaatttaatt cctatattga aagatacctt gtcagattta gataatacag
     2701 aggttgtaaa tcaagatata ctaaaagtag acatacagga attagtaaaa gataaattaa
     2761 atggtggacc agttaagctt gtagcaaatc ttccatatta tataactact ccaatagtta
     2821 tgaaattttt agaggaagac atacctgtaa cagacatagt agtcatggtt caaaaagaag
     2881 ttgcagatag aatgaatgct attccgggta ctaaagatta tggggcatta tctatagctg
     2941 ttcaatacta ttgtgataca gagattgtgg caaaagctcc aagacatatg tttataccac
     3001 aaccaaatgt agattccaca gttataggtt tacatgtaag agataaaaga aaatatgatg
     3061 ttcataatga agacatattt tttaaaacag taaaagcatc ctttggacaa agaagaaaaa
     3121 ctcttcttaa ttcgttggga ggtcttggtt ttcttaataa agatgaaata agagaaatat
     3181 taaaagaagc taatatagac gaaaaaagaa ggggtgaaac tcttagtata gaagagtttt
     3241 cagttctttc aaatataata aatacaaaag tatcttctaa atagaaggta ctttttttaa
     3301 ttttgttaac atatattgtt tagagaaact gtgttaaggg gggattgcag tgggttctaa
     3361 aagaaagttt aaaagagact atataatact agaagctaaa gacataaact ttagatacaa
     3421 agaacgagta ctgccaaaag cttttgcaaa agttgaagta aatgatgaaa aagcaattat
     3481 tgcattatat gtagaaaatc taaaatatgt taaagatggg tataaagcag tagcaataaa
     3541 agatgaccat ggattaatag acttaggaaa tataatttta aatgaacaag gaagaggtga
     3601 atttgtttta gatttagaag attctgatga tgagataaaa ggtattgcat tattgtatga
     3661 aagaaatata cctttaattg gatttaaggg aaataagctt gaaaattatg aggacatatt
     3721 atttcaagag gtagaggaag aagaatatga agaatatgaa gaatatgaag agttggaaga
     3781 agacgaagag atagaggata atgaggaaag tgatagagac gaagatagtg aagaagaata
     3841 tattatagaa gaatatgatg aagatgagta tgaagactat tgggaagatg atgaagatat
     3901 tgaagaagga gagttaaaag caagaacgga agtagaagat tctggtgatg aggaaataga
     3961 agagttcaat aattataagg aaatagaaaa ttataatagg gttaaagtac aggaagaata
     4021 tatagacata gaatatgatg aggaagaaga ggaagaagaa gaagtttttg aagtaaatag
     4081 agaagaagat aggtttgttt cctcaaaacc tactaaatca aagaaagttc aaaaagatca
     4141 gaataatatt gaaaaaataa aaagagctaa tggagtaagt caaggagaga gtggaaataa
     4201 acaaaccagt acattactta tgccaagaca aataaaaaaa ggtttgaaat tatttaaaga
     4261 agtaaaacct tttgtaacag attatataga taagacaaga tggtggaaaa tagagataaa
     4321 tcctacaaca ctgtgtggat atacaatgcc ttatttagga tatttaaacg tattgaatta
     4381 cactatgtat agtgatgcag ttttacattc atataaatac agacactatt tatttggtgt
     4441 tcagtatgat gaatataata agagaaaaca ttatatttat gctatcccag gaaatagaaa
     4501 tgaacagcca gacaatggaa gcacaggatt tttaagatat caagcttgtg ataatagaac
     4561 aaatagttta ggatattggc tatgttttat agattctaga caaagaaata tagttaggta
     4621 aaaataataa aaatgaaaaa atttagactc aattcttatt ttactgaaaa aattaatgaa
     4681 atgtagtaca aaactttaaa aactattgat aatatttaaa tattacacta caatatatct
     4741 tgaaatacct attatattat ggaggaaaat tatgataaac gagcaaagaa ttttaaatga
     4801 gtttcttgaa ttagtacaga tagatagttt atctttaaaa gaggggaatg ttgctaaagt
     4861 attagttaaa aaattagaag aaataggttg ttcagtagta atagacaatg ctggtgaaaa
     4921 agcaaatgga gaaactggta atataatagc tacattaaaa ggaaataaag aaggaaagaa
     4981 aatattattt agttcacata tggatacagt aactccaggt ataggggtaa aacctatagt
     5041 ggatgaagca aatggaataa taaaaagtga tggtactaca atattaggtt ctgatgataa
     5101 agcaggtata gcagctatat tagaaggctt aagatatata aatgaaaata acatagaaca
     5161 tactgatata caagttgtat tctctatatg tgaggaatgt ggattagttg gagctaaaaa
     5221 tttagactat ggaaaaatag attctgaata tgcatttata ttagatagtg gtggttctcc
     5281 aggtgaaata atagtaaaag ctcctgcaca agatgtaata aatgttaaga tattaggaaa
     5341 gactgctcat gcaggtcttg aacctgaagc tggaataagt gctataatgg tagctgccag
     5401 agcaatagaa aatatgaatt tacttagaat agacgaagaa acaactgcta acataggaat
     5461 tataaacggt ggaacagcta caaatatagt aacaggagaa gttaatatag ttgcagaagc
     5521 cagaagtcta aaagaaaata aattagatgt tcaaactaaa cacatggtag agacttttga
     5581 aaaagctgca aaagattttg gagctcaaat tgagatagat gtcaatagag catatacacc
     5641 aatagatgtt agtgaggatt ctgaaataat aaaacttgca aagaaggctt tttcaaactt
     5701 aggtatagaa ggtcatactg aatctacagg aggaggtagc gatacaaata tattaagtaa
     5761 gaatggtatc gaagctataa ctttaggaat tggtatgaaa aatgctcata cactaagtga
     5821 acatatagct ataaaagact tatatgactc tgctaaaatg gtagtggaga taataaagga
     5881 agcataaatt taaataatag tgtataatat atatatcgcc tagcttaagc taggtgattt
     5941 ttcattttat agaagattga taatctctat tgaaagcaaa gagtatacat ttgcatataa
     6001 gagagtgttt aatttatgtt cagatgtagg ttcttaataa tatttagatt gtttcataag
     6061 ggaggaataa atatggaaac aaggtatgaa gaagcaaata aaataacaat acaatcaata
     6121 ctttggaatg tagtattaac aataattaag gttatagctg gtgttattgg taattctagc
     6181 gctatgattg cagatggatt acattcagca tcagatatta taagttctat aggagtacta
     6241 ataggcaatt atgtatcttc tagacctgga gatagagagc acaattatgg tcatgagaaa
     6301 gcagagacat tagtatcatt tgtgttatca atattgctta ttttcgtttc tataacaata
     6361 ggtatagagg caattaaatc tttatttaac ttagatgcat taagtgttcc atcaatatta
     6421 ccattagtag tatcagttat ttcaatcctg ataaaagagt atcaatatag aataacaata
     6481 aaagttgcta aaaagataaa ttcaccagcc ttaaaagctg atgcttggca tcataggtca
     6541 gatgctttat cttcagtagc tgcttttata ggtataggtg gttctatttt agggtttaag
     6601 ccactagacc caattgcgtc aattgtagtg gctatatttg tagctaaagt aggaattagt
     6661 atacttataa gttctgttaa tgaattgatg gatgtttcag ttgacgaaga agagataaaa
     6721 gaattaaaat ttattgtagc agatacagaa ggtgttaaaa atcttggtga tataaagact
     6781 agaaaacatg gagctatggc atatgtagat ttgactatat gtgtggatga aaatttgaca
     6841 gttaagcaag gtcatgatat tgctaccaag cttgaaaaac atataataaa acatatggaa
     6901 tttgtaaaag gcataacagt tcacgttgaa ccatgtacta attgccaagg aaataagtgt
     6961 aataattaat taaaacttaa aaaaaagtgt aaaaagttat ataattgttt tatgattata
     7021 aactctaggg gtggttaaat gagcaagaaa agaaaactaa agaaaaaagc tgcaatatta
     7081 ataggagtag ttgttttctt tataatatac tttatatcct ttagaatagg aataaatatg
     7141 gaaaaggata aaagtgaagt gccaaagaag gtagaaaaag aagaagaaga taataggtct
     7201 ttaaggactc aaataaaaga aaagaaaaag gtttatatat cagacaaaaa tatagaaaac
     7261 ataaaaattg aagaagatat gtgggatgaa ataaagttat ttttctcaga ttttaagaaa
     7321 gtaagaaata ctacttcttc ttacgaagca gtttatgaag gttattctga tgatggagtt
     7381 aaattttcta ctgaccttaa cttttttaga gtatatactg taaataaaga agaatattat
     7441 aaagtaccag ttagttctaa ggatgctttt aagaaacttt tagatgagag tatatatact
     7501 tcatttgacc ttttaagtca ttataaagat tggaaagagg ttactataac tcatggaaaa
     7561 gaaattaaaa aagttcatag atggaaattt gatgatttag cacataagat gatttcaaag
     7621 agggttgtag gaaaagttca acctgagaaa agtaaggaaa gaagtgacta taattttact
     7681 ataaatataa aaggtgataa ttttgatgta aaagttgata caatgggaaa agattatgta
     7741 aaagttactt ctaagaaaaa tgtatcttac tatgaggttc atacaagttt atttgattac
     7801 cttaaaaatg agatatttga aattggaaag aaataagact ttaagaaggc tatcaaatta
     7861 gatatttgat agcctaaatt atttttatat attatttttc aaccaacaag tctgcaacct
     7921 tataaattgg accagcacca gcagttatta ctaaatcatt atccttaaca ttttcacgta
     7981 agtattttac tatatcttca aattctttta tatagatggc atctacattg ttttgatata
     8041 atttctcaac caagtcctta gaatgtatat ctccagggtc tttttcccta gcagcatata
     8101 tatctgttat gataacttta tcagcagcat agaatgcctc tgaaaattca cctaaaagag
     8161 attttgttct agtataagta tgtggctgga agatacacca taaagttgat ttttttaatt
     8221 ttttggcagc agctaaagtt gcttttaact cagttggatg atgtgcataa tcatctacta
     8281 ctaaagcatt cttataatac cctttagttt caaatcttct tccgacacct ttataaattt
     8341 ttatattttt tcttatagtc tctaagtcaa taccagaaac aagagctgcc attatagcag
     8401 aagaagcatt gtatatgtta tgaagaccat atacagaaag ttcaaattct cctaaatctc
     8461 ttccttcata ttctattcta aatattccat gaccactatt gtcaaagtga atatctttta
     8521 ttacagcatc attgtcagaa tctctaccgt attttataat agtagcttta acatcataca
     8581 atatgtcatc aatattttca tcatcgccat ttattatgaa atagccttct gggggtaata
     8641 attttccaaa tttgttaaaa gaagccttga tttcatctat tccagagaaa taatcaagat
     8701 ggtcttcttc aacatttaaa actatagata tctttgggtt aaaattaagg aaactatcta
     8761 catattcaca tgcttcagtt ataaagtggt tggaattacc tatcttaaca ttaccaccta
     8821 tcatacttaa gttaccacca actagtatag ttgggtctaa gtctgcatat tcaaagattg
     8881 tagataacat agatgttgta gaagtttttc catgtgttcc agaaactgct atagaatttt
     8941 tatattccct catgatttgg cctaaaaaag tcgctctgtt cattaccaat ttattttttt
     9001 ctttagcagc cacaagttct tcattgtctg gatgaactgc tgctgtataa acaaccatat
     9061 ttacatcatc tgatatatta tcttttttct gaccaatata aatagttgcc ccttggtccc
     9121 taagtttgtc taataggtat gattcgtttg aatccgaacc tgaaacttga taacctttat
     9181 tgatacatat ttcagctaat gcactcatgc tgataccgcc aattcctata aagtgtatat
     9241 tcataataaa accacctttc tttttaatta agtcctacaa tatgaactat ttatgtggta
     9301 taataatata atgttcaata ttttataatt aatatgcata taatattata tgtattttat
     9361 ttttgaatat aataaaacat aagtaatatt ataccacaat aagaattgtt atgcattagt
     9421 tagtttaacg ttatataaag gtacttgtag gaggaaataa taatgaaatt taaaagaaca
     9481 gagagaattg gagcaatagt taaaatactg tctgataatc caaataaaat atatacatta
     9541 agttatttta caaatcaatt taatgcagca aaatcaacaa taagtgaaga tttattagtt
     9601 gtaaaaaatg tatttgagaa gctacattta ggtaaagtta taactatctc aggagcagca
     9661 ggaggagtaa aatatatacc taaaacttct atagcagaaa atgcagagtt cttaatggaa
     9721 ttatgtgaga gaatatgtga caagtctaga atactatctg gtggtttttt atacttgatt
     9781 gacctaattt atgaccctac aatagctgct aagataggaa agatattcgc atcaaatatt
     9841 gagtatgtag atgcagatta tgttgtgaca atggaaacaa aaggtatacc tatggcactt
     9901 atgactgcaa aagctatgaa tttacctcta gttataataa gaaaagatat aaaggtatca
     9961 gaaggtccta cacttagcat gacatatgtg agtggaaaca gctctaaagt tgaaagtatg
    10021 agtttgccaa gaaaagctct taaaccagat agtaaagtta taattataga tgatttcatg
    10081 aggggtggag gtactataaa aggaatggta gaccttatga atgaatttgg tgcagaggtt
    10141 ataggtactg gagtattcat atctacaacg aatccttcag aaaaaatggt aaaagattat
    10201 atttcattga ttcaattgga tgttaatgga gataaaatag tagtagaacc aaacttaaag
    10261 acttttaaag atgaatatag aaatgaagat ttagacgaag aagaagattc agaatttgaa
    10321 tttgagatag atgaagatta ataaaaaaaa tatttagaaa aatatgtaaa aataaaaaga
    10381 ggatatccct agttgttcat agaattattt aaaaaacagt ttatattaat ttattccagc
    10441 gataataatt ttaatataaa ttaatttctg tcaaaatcaa agggggatat tttggtatga
    10501 agataactga cgtaagagta agaaaattaa cagaagaagg taagatgaaa tgtatagttt
    10561 caataacttt cgataatttg tttgtagtgc atgatataaa agttatagaa ggccataatg
    10621 ggctatttat agcaatgcct agcagaaaag taggcgaagg aaatttcaga gatatagctc
    10681 atccaataaa tgcagaaatg agacaagttt tagaagatgc agttttacaa gcatatcatg
    10741 aagcattagt tcaatgggaa gtagctgctg aataataaat atataaaatg gagagagccc
    10801 gaacatttag gctcttttta aatgtagaat ttctattaaa atatgtactt tttagtaaaa
    10861 aagtgctata ttattagtga taatgtatat gttgtataca aattgttaga taaatgtatt
    10921 ataatgtata ttgattaaga gaataatacg aataaaatta aattagattg aatggagtgt
    10981 atcaatgaac tttaaagcta taatccttgc tgctggcaaa ggaacaagaa tgaagtctaa
    11041 atacccaaaa gttatacata aagtatgtgg aaaagaaatg gttaaccata ttatagatgt
    11101 ttcaaaaaaa tcaggggtaa aagatactgt cgtgatttta ggacatgaag ctgaggtagt
    11161 taaagaaaag cttgcagaag aaattataat agccatgcaa acagaacaac ttgggacagg
    11221 acatgctgta aagatggcta aagaatatat taatgatgaa gatacaatag ttgtactttg
    11281 tggagatact ccacttataa aagaagagac actgaagaga ttatttgaat atcatataga
    11341 aaataaatat catgctacag ttcttacaac aagagttgga aatccaacag gttatggtag
    11401 aataataaga gataagaagg gtgaccttct taaaatagtg gaacaaaaag atgcaaattc
    11461 agaagaaaag atgataagtg agataaactc tggaatttat tgttttaatg gaaagagtct
    11521 tagagaagca ctagatttgc taaataataa caattcacaa ggtgaatatt atttaactga
    11581 tactgctaaa ataatgagag ataaaggatt aaaagttggt gcatttgcag gttcaactat
    11641 tgaagaactt atgggcgtta attcaagagt agaattatca aaggcagaag aaattatgag
    11701 acgaagaata aatgaatctc atatggtaaa tggagtaact ataatagaca ctaactctac
    11761 ctatatagaa tctgatgtta tgataggcaa tgatactata atatatccag gtgttatgtt
    11821 acaagggaaa acaaggatag gttcagattg tataattgga atgaactcat ctataacaaa
    11881 ttcagaaata ggtgatggta cagaaataaa aaattcaact ataattgata gtaaggttgg
    11941 agaaaattct aatgttgggc catatgcata cttaagacca aagagtgact taggaaataa
    12001 tgtaaaaata ggcgattttg tagaagtaaa aaatgctatt atagaagatg gatcaaaagc
    12061 atctcacctt tcttatatag gagatgcaca tgtcggtaaa aatgtaaata ttggatgtgg
    12121 agtagtattt gtaaactatg atggtaaaaa taaatttaaa tcagtagtaa aagacaatgc
    12181 gtttataggt tcaaattcta atttagttgc accagtagtt gtagaagaaa aaggatatat
    12241 agcaacaggt tcaacaataa cacatgatgt cccagatggt gctttggcaa tagcaagaga
    12301 aagacaagtt atcaaagaag gatgggtaga aaaaaagaat caaaaagacg accaaagtaa
    12361 gtaattatgt agtttattat tttggctaaa aaatttagct cgaaattatt ataaattata
    12421 ttcattagta ttttaaaatt ttcaggaggg ttacataaat gaatactagc ggaagcgaga
    12481 ttaaaattat tgcaggtaat tcatcgaaag agttagcaca aaaaatagct gactatatag
    12541 gtgtatcagt actagattgt gaagtaggta catttagtga tggtgaaata tgtgtaaaca
    12601 tgaatgagac agtaagaggt tgtgatgtgt ttgtagtgca atctactaac agcccagtaa
    12661 atgataactt aatggagtta ttaatcttaa ttgatgcgtt aaagagagca tcagcaggta
    12721 gaataacagc agtcattcct tactatggat atgcaagaca agacagaaag gctaaggcaa
    12781 gagatccaat cactgctaaa ttagttgcaa atttaataac tgctgctgga gcagacagag
    12841 tattaacaat ggatttacat gcagctcaaa tacaaggata ttttgatata ccattagacc
    12901 acttactagg tggaacaata ttagcaaatt attttaatga aaagaaaata gaagatttgg
    12961 tagtagtatc tcctgactta ggaagtgtta ctagatcaag aaaatttgct aatactttaa
    13021 atggagaagt tccaatagct ataatagata aaagaagacc gaaagctaat gtatgcgaag
    13081 ttatgaactt aataggtgat gttaaaggta aaaatgttat actattagac gatatgatag
    13141 atacagcagg aacaatagtt aatgctgcaa atgcacttaa agagtttgga gcaaaagatg
    13201 tttatgcttg ttgtactcat ggtgtgttat caggtccagc tattgaaaga attgctaatt
    13261 cagaaataag tgagttaata gttcttgata ctatacaact tcctgaagag aaaagaatag
    13321 ataagataaa gataaaaaca gtagctcctt tatttggaga tgctataaga atgatattct
    13381 ctaatgaatc agtaagtaaa ttattctaat ttattgtatt tatgagagtt gtttaaaagg
    13441 atataaacct tttgagcaac tcttttttga ttttatactt atggaatgtt atttttttct
    13501 tgttagaaag gtatatatta ttgtaaaaag agattatagt ttaaaattat gtatatttaa
    13561 gtaaaataaa cttaataaag ggcatattat tcaaaatatt atgaatataa aaaatattac
    13621 aaaaaagtat tgaaaaaatt cagattatgt agtattatgt aaatataaat aaagaaagag
    13681 gtgtttccaa tgggaaggtt agaagatgaa cccctaataa ttaaatgatt cagtcacaat
    13741 ctaaagtgaa tataaaaatc tattgaaagt ttatttataa gaattgaaaa tagtatctat
    13801 tctatcttta gtgtttgtga gagggttctg gattatataa gattactgta aagagtaatt
    13861 ttgcacaata agcatagctg taaatgaaga gtttcgttta tataatgatt gtggctgaat
    13921 cataactata atagatagaa tgtttataat agatgatttt ataaaatact atattgtaat
    13981 agcttttagc tttcaaataa atattagcaa aggatggaaa tgacaatgat aaaactaaat
    14041 gataatttat aaatatctcc ttacgatata taataattgt agggagatgt ttcttttttt
    14101 tgtagagtat aatttttcta gaaattctgt ttcaatatgt aaaaagttgg tatatataaa
    14161 tagtataaac agtgttgcta aaatatggta gatattatat aatataagtg gatatgtata
    14221 tacattatga gttatataat tttgaattta ataaaataga aacgttgatt tatgttctgt
    14281 aatgtgggca ccttagagca tattgagtta gtggtgcaac cggctatgaa attgtattta
    14341 tttatagata ctatcatttt catagctttt tttatttaaa aaatggttat gtgttagaaa
    14401 taaatttggt tagtacgttt aaataatttt acttagattt ttaaccagat atattataag
    14461 taagtattaa ttaaaaaatt taagggggaa taaaaatgaa gttaaaaaag aataaaaaag
    14521 gtttcacttt agtggaatta ttggtagtaa ttgcaattat aggtatatta gcagtagtgg
    14581 cagttccagc tttatttagt aatataaaca aggctaaggt agcaagtgtt gagtctgatt
    14641 atagttcagt taagagtgca gcattatctt attattcaga tactaataag ataccagtta
    14701 caccagatgg tcaaactggt ttaagtgttt tagagactta tatggagtct ctgcctgata
    14761 aagctgatat aggtggaaaa tataaattga ttaaagttgg taataaattg gtattacaga
    14821 taggtacaaa tgatgaagga gttacattaa cagaagcaca atcagcaaaa ttattgagtg
    14881 atataggtga aaataaaata tatacaagtg ttacagcaga taatttggga aatccattaa
    14941 caagtaatac aaaagtagat aataaagttc tatatatagt acttatagat aatactgtga
    15001 tggactcaac aaaatagtta aaactatatt tttataaaaa tattgtaaat ttaatgaaaa
    15061 atataatata aaagacagga agaaaatctt cctgtttttt tatactattt tctttaatgt
    15121 tcaattatga atgatgatat aattgagata gtaaaggagt gaaacctgtg gctaaaaaag
    15181 ttagaatagg agacaaactg gtcgaaaaag gatatataac agaagaccag cttaaatggg
    15241 cactatcaga acaaaaaaac agtggaaaaa gactaggaga atttctagta caagaagggc
    15301 ttatagatag taacttacta ataagtgtat taaaagaact cttggatata gaaagtatat
    15361 ttttagaagg gacagaaata gacactttag ctacaaagat ggttccagaa aatatatgca
    15421 aaagatatac tgtatttcca tttaaaattg atggaaataa aatatgtcta gccatgtcag
    15481 acccacaaga cagagaagca gttcaagatg taaggcgtat gtctggtaaa gacgtagaga
    15541 tatttatatc aagtacagaa gatataaaca aagcaatagg tcatgcttat gcacattcag
    15601 aaataaataa ggcaatgact gagtacaaca aaaatagaac tggtggagta agagaaacag
    15661 taatacttga agaagatgta aatgctgctc caatagtacg attagtaaat aacatactag
    15721 aaaatgctgt tagaatggaa gcaagtgata tacatataga acaaagtgaa aattacatgc
    15781 gtgttaggtt taggatagat ggaatgttaa gagaatatat gaggatgaac tcagcaccat
    15841 ataaggcagt aatcagccgt atcaagataa tgtcagatat aaatatatct gaaaagagaa
    15901 taccacagga tggtagaatc tatttaaaag tagataataa acccatagat tttagggttt
    15961 ctacaatgcc aactaacagg gatgaaaaga tagctatgag ggttcttgat aagagtaatt
    16021 ttatggttag taaagaagta ctaggaatag acgaacatgg ctctaagata tatgatgaat
    16081 tgataaacac tccttatggt cttatattgg ttgttggtcc tactggaagt gggaaaacca
    16141 ctacattgta ttcaatgtta aatcaactaa atacagaaaa tagaaatcta ttaacaatag
    16201 aagaccctat agagtatgaa ttacctggtg taaatcaatc tcaaataaat gaaaaggcag
    16261 ggcttacttt cgccagcgga cttagagctt ttatgcgtca agacccagat ataataatgg
    16321 ttggagagat aagagatact gagacagcag aaatagcaat tagagcttct cttacaggac
    16381 atttagtttt aagtacatta catgcaaata cagcagttgg agcaatttca cgtcttttag
    16441 atatggatgt ggaaagtttt ttaataactt catctgtttt aggtgtaata tctcaaagac
    16501 ttacacgaaa aatatgtgag cattgtaagg tttcatatga agcagatatt ggagaaaaaa
    16561 aggctcttgg tattgatgta aatgaatcag taactattta tagaggaaaa ggttgtgaaa
    16621 ggtgtaataa tactggatat aaaggacgtc taggtatatt tgaaatgctt gaaattactc
    16681 cagaaataaa agagttaata gattcatctg caaatcaaag agaaatttta aagatggcta
    16741 gaaaacaagg catggttagc ttaaaagagg atatagttaa aaaggtttta aatggaaaga
    16801 caacagtaga agaaatgatt agaataattt tgatgactga ttaaaggatg atattatgaa
    16861 aagttttaaa tacacagtaa taaaagagga cggaaaaaag caaactgatg tgattgaagc
    16921 caacgatttt aatgaagcta gaaatcttct tagaaagaga aatcttagag ttattgaaat
    16981 caaagaaagc aaaaataaaa atataagatt atctagtaga aagagaaaaa aagacttggg
    17041 agctgaccag ataagccact tttgtagaca gtttgctatt atagtatctt ctggtataaa
    17101 tagtatttca ggattagaaa cattagctag aaggtcaact aacattacat taagagaaga
    17161 aattaatcgt atagtggcag aaataaaagt aggttctact attgcagatt caatgctttc
    17221 atcaaaatca aagtttccaa agttacttgg tgctatggta gctacaggtg aagctacagg
    17281 taaattagat gaagtattaa aaagtatggc ttcattttat gcaagtgaac acagggttaa
    17341 acaaaagctt agaaatgctg caacatatcc attaatagtt ttgatttctt catttataat
    17401 gatttttata tttacaacat ttatggtgcc taagatgatt aattcaatta caacagtagg
    17461 agcaactctt ccacttataa caagaattgt tatggggttt ggtatgttta tgaagaagtt
    17521 ttggataata gtacttttag ttattatatt atttatctat cagtttaaaa aatatttaaa
    17581 aacgtcagtt ggaagggctc ataaagatag agttataaac aagatacctg ttttaggaaa
    17641 aggaataaat tgtatggtag ctacacgatt ttcaagagca ttgtatctat ttgtatcaac
    17701 aggataccca ttagttcaag ggcttgatta tattatagat agtgtaaata acaccatggc
    17761 agagaagatt ttagcatcag ctaaagatgg aataacaaga ggagaaggct tagcagaaaa
    17821 tttagagaga tatacgtatt ttgactctgt gttggtacag atgatagcta taggggagca
    17881 gacaggggaa ttagagaata taagtagaca gatggcagaa ttttatgagt atgaatccga
    17941 gatttattta aataggatgg cctcaatgat agagcctgtt ttaataattg cagttggtat
    18001 tatagttgcg atactggttg tatcaatatt tatgccaatg cttagtatat atgatgccat
    18061 gtaggaggga aaggacaaat gaataaggtt tatgtatcat attatggcga ttctatttcc
    18121 atagttgagg gcagatataa acacgataag gataagtttt atgtaaaaaa ttttaatgtg
    18181 ttttcaatag aggatattgc acaagatttt tctaaggaaa agtatgctct attgagatac
    18241 gcattagaaa atgttaagtt aaaatcaaga catgtagttt tttgtttaaa tacaagagat
    18301 gtaattttaa aaccccaaaa acttcctaat atggacaaaa aggatttaga tggatttatg
    18361 aagctagaga tggacgaaat gatggctttg gagtcagatg aatatgtatt ttcgtatgaa
    18421 gtatctagtg aaacacatga taattcagat ggcgatgaat caagcttaaa tcttatcatt
    18481 ggagctatac aaaaggaaga ggtaaatgca attgtagaga taatgcatga atttgattta
    18541 gtattagata gaatagatac attatcaaca gcttacttaa gaatgcttaa aaatttagac
    18601 tacaatgata taatggtaac caatataagt gacaatagta caattataaa tatatataag
    18661 aaagatactt tatttattag tgataatata cctataaaga taactgctaa agatatggac
    18721 atgcaattat taaatgtagt agaagaatca aaaggtctta tgaattacta ttcgtcaaga
    18781 aactttggta aggttacgga tactatatta atattaggaa aaagatattc caataaatta
    18841 atttatgaaa cttttagctc tgcattttca agctatgttt ctcaaggcct tactgagata
    18901 atggatatat ctgatatgat aactggagat atagatgaag atgatataaa ttcaattgta
    18961 gagcttttag gaagtatgat agaacctaaa gggaaaagag attttgaaaa tataaacttt
    19021 ttaccaatgg atatacttag aaaacagata aaagctaaac aaatgaaaaa catactgaaa
    19081 attgctccaa tagttatttt gattcttgct ataccttatt tatgtttaat tttttcaaat
    19141 aatttagcaa ataaggaact tgaaaatgtt aatggtgaaa taagaaatat tgaatctgca
    19201 tattatcaga ttggaaatat agaaaaaatg atacagagta aaagcgaaga aataaaggta
    19261 tatgatatgt taattgggaa aaaggtaaag tgggaaaatt tattggatga tatagataaa
    19321 aatactccaa gtaaagttga actaacaagt ttatcaacaa cttatcaaaa tcttggaact
    19381 aataataaag ctgataattc aaaaagtaat aagactacaa cagataaatc aagtaacaat
    19441 gaaacaaaat caagtacgaa aagtgatagt aatggagata aaacagataa tgatttaaat
    19501 gaagataaga ctacaaatgt gtcagaagca ttaactggaa atagtgagac taatagtaat
    19561 aacacggata aagatgataa aactccattg tatgataaga taccaaatgt tataaatata
    19621 gaaggaaatg ctaaagattc ctcttatgta ggtcaatttt tatataaatt aaagggatta
    19681 tactatttca aggatgttaa attacatagc ataaaaagta atgcagaaga ttctaactat
    19741 acatttagta tgacattata cctgaaagaa ggtgtattaa ctaatgaata aagatatatt
    19801 taaaaaagat ataaaagata tatttaaaat ggaagttagt ctaaaaggga ttcttataaa
    19861 ggttataatt ttattagcta ttttatcata tggttatttt ggactttacc ctcaatatca
    19921 gaaatatatt gctacaaaaa attctgtaaa acaggctact gaaaaattaa atatatataa
    19981 agagaaaact gcacttatgc caaaatatga aaaacaactt gaagaagttg aaagtgaatt
    20041 aaaagaaaaa agcaaaaatc ttttatatga tatgcaagat ggtctttttt taataggttt
    20101 agataaaaaa atgaaggctt ataatattga cttaactagt tatgacgttg gaaatgttaa
    20161 agaatacgaa catttttatg gtatatcaac aacaataagt gttagaggtg attatagaaa
    20221 tgtcaaaaaa ttaatggctt atttagaaga acaaaaaaat gttactcaaa tactagatta
    20281 tacaatgact tcatatatag aaccagaaaa agaagaggaa gaaacaacaa acaataagga
    20341 tttaattaaa aatgtgaaca taaataaaga tacaaaaata tactgggtgg aaggcgaaaa
    20401 taagtggcat ttatataagg attgtccatt gataaaagaa ggagagattc taaaaaatga
    20461 caaactttta gataggtcta ctaattatct atgtaatact tgtgtactta gagctgcaac
    20521 agaaaaagaa gcaaaaaaag aagtcgtagc tccaaaggca gaaggtgtag tagatgctac
    20581 atttaaattt ataatgtaca ctaaagagaa tactacaatg atacttgata cagataatcc
    20641 atcttcatgg agaccaggaa aatataatcc atttaagtct agtgctaaat aaagctaagt
    20701 aagctttaaa gataggtgat gagtttgtac aaaaataatg aaaaaggtct tactttatta
    20761 gaagtaataa tagctgtttt tatactgaca atagttttaa gtatttctta taaagtgttc
    20821 aatggtataa catcagcagt aaaaaagcaa cagattatta cagatgctca agtaaatatt
    20881 aatctaatta ataagtatct aaatagagat ttggaaaact gtaaagagct aactaaaact
    20941 ggttcaggta ataattatga atacaatata gagatgccag ataatgttgt aaaatatgaa
    21001 gtttctatag aaactaaaaa aaatacagag gtatattctg taacgagaat ccaaaacaat
    21061 acaattgata cagaaaatga agttagagaa gagataatct ataatcaacc actagttcaa
    21121 aacaataaag aaatgaagga aacaccattt aaaatagaaa agcaaactgg taaatctatt
    21181 tatactgtaa gtatatacta taatgaatca gtgcaagaaa gtcataaaaa tagcaattta
    21241 aataacaaaa cttatacatt tgatgttatg tcaagaatag ggtaataaga atttattaaa
    21301 aaataagtag gttgataaaa tatgagtaaa aagaatagtg aaagaggatt ttccttaatt
    21361 gaagtgttag tagctatggc tattatggga attgtactat ttgcattttt taatattatc
    21421 aatactaata acaaagcaaa cactaaaaat gacacagata taacttcctt aaactatgtc
    21481 caaagtgaaa tagaaaatct aagagaaaag ataaaaagtg gagaatttga ttttgatagt
    21541 ttagataaac tagaagatgg aactgtcgta tatgaaaaat taatagataa atcaaaaaaa
    21601 gttgtatatg ataaagttct tagtgaaggt gacgtgagct tatatgatac tccatatgaa
    21661 aagattacaa caataaaaga tgaagatggt aatttaatcg acaaaggaaa tattacaaat
    21721 aagataaaga ctattgtaga agataaaagt ggtcagatat ataaaatagc tgtaactggg
    21781 aaaagcatga atgactattc aagtaaaaaa gaggttaaaa ttgtaactga aatatttaaa
    21841 gataaataat tttacttggg ggtgagattt tgaggaagtg gaataaattt aaaagtgaaa
    21901 gaggggcagc tttagtttta gttttgattg tagtagcatt gctttctatt gttggattga
    21961 tattttcaaa tcagatagct aatagaataa aatctacaaa gactactaat gaaggtatac
    22021 aagcaaagta tttagcagag acatgtgtgg aaaatagtat tgataaagca tatgaaaaat
    22081 tgtatgatga gcttgagaaa atggataatg agtttaaaag tgaaaatcaa gaaaaatcca
    22141 tatctaggtc aaaattaaga aatatatcag atgaagattt taataatcaa gatgagaaaa
    22201 atattgaagc agaaagactt ggatatatga ataatataaa tttctattta aataaagctt
    22261 cttcagactt ggaaaaagct agtatggaat tgaaaaagct atatgattta gatatgttgg
    22321 actatagaga cattgaatat gtagatgcaa atattatttc acatagagat tctatcttag
    22381 aaatttgcaa gaattatact tctggagata ttagtaaaat aaatgaatat attctgaaag
    22441 aagatataga ctcaactaca ttgattgagg caaagttagt gaataatgat attcttttga
    22501 aaatgttttt agaagaaaat aaaattgaaa atgaacattt aaattcagcc ttttcacata
    22561 cttataaggc attagataac atttctttgg cgatgcaaaa tatgattgag tatagacata
    22621 cgtttcatat agatgagcca aaagtagaag tatctaatgg gattccagat tctcaacaat
    22681 attatgaact aatacaaaat cctataataa atagtatgga gtatatctgg aatagtaaat
    22741 gggatacatt agaaaaccta ttggaaattt taccaaatca aactcaagga tttaattctt
    22801 taagagttca tttgagaaat aatgtaagaa aatttgagaa gttaagcgat aatatatcta
    22861 gtggtaaaaa aaatacagca aaaaattttt taaagtataa agagttattg tatgaaatat
    22921 cagaccaatg taatcaacta aaaagtatgt cttatgagaa aatacctgtt aaatatgata
    22981 atatggcttt gataacaact tttgactata tacaaaatga gcttttagca gagataaaat
    23041 gcagattaaa agaattaaaa ccacaagaaa ttgataaaac agaaggtatt actataaaaa
    23101 taccttttta taaggctgac tacgacatga caaaagaagg atggcctaaa ttaaaagaaa
    23161 atggttcagg ggcggagtta agcttaatgg taactggtga taaagatgga ataaaagaag
    23221 tagaagttac agatggtaaa aaaaatatta ttggtttagg tgttgaagaa aattcaaatt
    23281 caaagtataa ggttgacgcc attgtaaact ttaatctaaa tatagataca aatgtagttg
    23341 gaaattatga tataaaagat aaaattttga ttaatcatga tataagttca tacaaaaaag
    23401 taaactaaat ataagaagca acttagtata aataaataat aaaataattt tattatttac
    23461 tactaaaaaa ataagtaaac taatagaaga tatggagagt aaatattatg tatatttatg
    23521 atttattaga gcaaggtata agactaaacg catcagatat acacataaca gttggaacaa
    23581 atcctgtagc tagagtaaaa ggtggatttg taaaactaag tgaacaaata ttgacatcag
    23641 aagtaacaat gcaaatggca aaagacatag cgggggaatc tatttttaaa gtaatagaag
    23701 aacatggaga agctgatttt tcagcttctc taaaaacagg agaaagattt agggtaaatg
    23761 catataggca aaaaggaaat tacgcaatag caataaggac tataacagca gaaattccaa
    23821 catttgaaaa attaggcctt ccagagagta taaaatcttt tactgaaaag cataaaggat
    23881 tagttttagt tacaggtcca acaggaagtg gtaaaagtac aacacttgca agtatgataa
    23941 atataataaa tgaaaaacaa caaaaacata taattacact tgaagacccg atagaatatg
    24001 tacatcatca taaacaaagt ttagtaaatc aaagagaagt aggaactgat acagaaagtt
    24061 ttcattcagc ccttagagct attttgcgtc aggacccaga tgtaatacta ataggagaga
    24121 tgagagaccc tgaaactgtt tcaatagcat taacagcagc agaaacaggt catttagtat
    24181 tttcaacact acatacagta ggggcagcta agacaattga tagaatagta gacatgtttc
    24241 aaccatccca acagcaacaa ataaaaacac agttatctac agtttgtgaa ggtgtggtat
    24301 cacagcagtt acttcctaca gcagatggga aaggcagaat agcagcaatt gaattgatgt
    24361 ttgctactcc agcaataaaa aacttgataa gggaaggcaa aacatatcaa attccaaaca
    24421 tgatacaaac tggagttaaa gtaggtatga aaactatgga ccaagattta atggaacttt
    24481 ataaaaatgg aaagattaca aaagatatgg cacttagtag gtgtactgac caagagttta
    24541 tgacaagaat gataggtgga gtaaactaca atggatatta taatcgttaa tctatatgta
    24601 tttatagtgg gtatagtgtt tggaagtttc tttaacgtct gtatttttag gattccagaa
    24661 ggtatatcta tttctagacc tccttcccat tgtatgagtt gtaatactag actaaagcca
    24721 atagacttaa taccagtgct aagctatttg atgagtggca gaaagtgtag atattgttat
    24781 gagaaaattt ctagtagata tgctatagtg gaattaatta caggaatttt atttttaaca
    24841 gtatttatgt tatatggaat atctacatca actatttatt atttggttct tgtgtcttta
    24901 ctgattataa ttacatttat agatattgac cactttataa taccagataa aatattgatt
    24961 tttggagcta tattttctat gatttttaac cttttattta aagaaattcc agttaaggat
    25021 tcaattctag gtgtttttat ttgtggagga agtgtctgga ttatagttct tttgattgaa
    25081 tttgtaataa aaaaagagtg tatgggtgga ggagatatca aactatttgc aatgctaggc
    25141 ctttacatgg gtgttaaaaa tggtcttttg acagccttac taagcgttta tgtaggagca
    25201 gtctatggaa tctgtgtaat aattataagt agaataaaag gaaaggaata taattctgtt
    25261 ataccatatg gaccttttat atctattgga gcattaataa caatcttatg tggaaagcaa
    25321 ttacttgaac tttatttttc tatggttata tagatgatag tcaagttaaa taaagtttta
    25381 atttaaaaaa atgaattaaa gtatttaata aggacaatat tattattaac cctattacta
    25441 aagtgctaca aactatggag tatataataa taatttcctt attttttgca catttgataa
    25501 ataaatctct atgtgttttt tgtgatagag aaaagaaaaa tttatataat acgtttttta
    25561 tttacataat aactgtagtt ttatctgtat taatatatct aaaatttggg attagtgtag
    25621 aatgtgtaca atatatggtt ttaattccat ttttaataat tatatccatt gtagattact
    25681 acacaatata tgtatatgat ataacaatag ttagtggtat aatagttcaa ggtataattt
    25741 ttttggcctt tttcaatata gctcaatttg attatataaa tcatatattt ggactattga
    25801 taggctttat tataccttat attattgcta aactaacaaa aggattaggc tatggagatg
    25861 taggtctcta tagcttatgt tgctttgcat tgggaaataa ttatgggttt tatgtgatag
    25921 ttttatcatt tattttagcg tttttgtttt ttattatttt atacataact aaaatattta
    25981 aagatgtaga tataagaaag caaattcctt ttacaccttt tatttccttg tcaacaattt
    26041 taataatatt tacagagtat aatatattta atttttatta taatttgtta tacaaattta
    26101 tataacagga ggtaagaaat gtacgtagta gtagggttag gtaatccagg aaagaagtat
    26161 gagaagacta gacataacgt tggatttgat gttatagata ttcttgctaa agaatacaat
    26221 attagtgtta ctaaaataaa gcataaagct ttgattggtg aagggagagt aggtactgaa
    26281 aaagtattat tagttaaacc acaaacttat atgaatttaa gtggagaaac attaatagat
    26341 atatataaat attataaggt tgatttaagt aatatagtag tagtatatga tgatatagat
    26401 ttagaagttg gaaagatacg tataagaaaa aaaggaagtg gaggaactca taatggcatg
    26461 aaatctataa ctaagtgcct aggttcaaat gactttccaa gagtgagagt aggtgtatct
    26521 aaaccagaag ctggtcaaga cttagcagac tttgtacttt ctagatttag aaaagaagaa
    26581 tctgataaca taaatgaagc attagaaaaa gcggcagatg ctattgactc aataataaga
    26641 gaaaatatag atatgtctat gaacaaatat aatggatagt gagagggaga tttaaatgaa
    26701 tgatgttttt ttatatcctt tgcaaaattc aaaagagtat aaagatataa taaactgtat
    26761 aaaaaataca aaaggctcat tattggttaa tggactttta ccagtacaaa aacctcatat
    26821 atcgtattct atatttaatg atttatcacg acaaatgatt tttataacga gtagtgattt
    26881 agaagcaaag aaagtatacg aagatttaag tttttatatg gaagataaag ttgaatattt
    26941 aggttttcaa gatatttatt tttatcactt ggatgcaaag gacagaaacg aagaagcaaa
    27001 gaaattaaaa gtattgttaa aactagcaaa taaagaaaaa atcattttag taacttctat
    27061 tgaggcagtt ttaagaaaat acataccaaa acaagttttg ctcgatagtg tatctcatta
    27121 taaggtggga gatagcttag acttagaaaa gttaactgag aaattagttt cattgggata
    27181 tgaaagagtt tcaaaaatag aggggtttgg tcaatttagt ataagaggtg gaattattga
    27241 tgtattttca cttgaatata caaatccaat tcgtatggaa ttatttgatg atgagataga
    27301 ttccatacga acttttgacg tatattcaca aaaatcaatt gataaattgc aacaattctc
    27361 tataactcca tctagagaat ttatttatcc cgaaaaaact acagatgcat tagttaaatt
    27421 aaaaaaagaa acaactaaaa atactgatga aggtacattt caaaatatag attatatatc
    27481 aagtaagact tattttgaag gtgcagagaa ctatatagat tatatttatc cagaagaaaa
    27541 caaaagtata tttacttatt tagcagatga tgcaatagta tttattaatg atataacgag
    27601 acttaaagaa agatgcgaaa attatataaa tgagtttaga gaaaattata aattaaatct
    27661 tgaaagaggt ctggctatta aaaaccaagg taaattgtta tatcactata cagatttaga
    27721 gtacttagta aaagacaagt ctgtggtatt aaactcgcta ttgccaaagt ctataaataa
    27781 cttttctata aaatctataa taaattttga aagtagagaa gttcctacat ttaatggaaa
    27841 ggtagatttg cttgtagaag aattaaatcg gctgaaatat aatggataca aaataatttt
    27901 agcaacaaat accttagaaa gagcaaataa attaggaaaa gatttattgg acaaaggctt
    27961 agaaacgacc atttctaaag atagagatat tgaaattaaa tcttcacaag ttataattgt
    28021 acctgcacac ataaacagtg gttttcagta taaatctata aagtttgttg ttataacaga
    28081 taacgaaatg ataggtgttt ataaaagagc gtctaagact agcaataaaa aagttaaaaa
    28141 aggaaagaaa atagaatcct ttttagattt aagtgttgga gactatgtag ttcatgaaaa
    28201 tagtggtgta ggtagataca cagggattga acaaattaca gtcaatgcta ttaaaaaaga
    28261 ttatatgaaa atagtatatc aaggtggaga taatctatat gtgccaatag accagatgga
    28321 caaagtacaa aaatatattg gtgcagaagt agaaaaagtt aagttaaata agcttggaac
    28381 aaatgagtgg acgaaagcaa aagcaaaagt taaaaaagaa attgaagata tgacaaaaga
    28441 cttgattgag ctttatgcaa aacgtgaaaa aatacaaggg tataagtttt caaaagatac
    28501 accttggcag gcagagtttg aatcgttgtt tccatatcaa gaaacagaag accaattaaa
    28561 ggctatagaa gaaactaaaa aagatatgga atcaagtaag gtaatggata ggctagtatg
    28621 tggagatgtt ggttatggaa aaacagaggt agctattaga agtattttta aagcttgtat
    28681 ggaccaaaaa caagttgctg tattggttcc aacaacaata ttggcccaac aacattataa
    28741 tacattcaaa gagaggtttg aaaattatcc tttgagagta gaagttttaa gtaggtttaa
    28801 aacaccaaaa caacaaaaac agattattga agatgctaaa aaaggattag tagatatttt
    28861 aattggaaca catagaataa tatccaaaga tataaattta ccaaacttag gtcttgtagt
    28921 cattgatgaa gaacaaagat ttggagtaaa acataaagaa gctttaaaga aaattaaatc
    28981 tacagtagat gtacttacac tttcagcaac tcctatacca agaacattac atatgtcttt
    29041 aagtggaata agagacatga gtgttataga agaacctcca caagaaagac atcctgtaat
    29101 aacatatgtt acagagagta aggaaagcgt aattcaagat gagatagaga gagaattaag
    29161 tagaggtgga caggtatttt ttgtatacaa tagagttgaa cacattgaag aaatggcaag
    29221 tatgattcaa aaactcgtac cagatgctag agtggctgtt gcacatggaa gaatgacttc
    29281 taaatcatta gaaaatataa tcctaggatt tttaaataaa gattatgatg tactagtctg
    29341 tactacaata attgaaactg gtatggacat atcaaatgca aatacaatga ttatatatga
    29401 tgcagataaa atgggattgg ctcaacttta ccaattgaga ggtagggttg gaagaagttc
    29461 aagacaagga tatgcatatc ttttgtatga aaaagataaa actttaagtg agatagcgga
    29521 gaagagatta aaagctatca gagaatttac agagtttggt tctggtttta aaatagcaat
    29581 gagagacctc gaaataagag gagcaggaaa tattttaggt tctcaacaac atggtcatat
    29641 ggcagttata ggttatgatt tgtacgttaa aatgcttaat gatgcaatta aaaaagtcaa
    29701 aggtgaacct attgtggagg aaattgatgt tgaaatagat ttaagtgtaa atgcatacat
    29761 tccagataat tatatcaaag atgagttgat taaaattgaa atgtataaga aaatagcttc
    29821 tattgaaaac aaagaagaca tgttggatat acaagaagaa cttgaggata gattctctga
    29881 tatacctaaa ccagtccaga cacttcttac aatagcttat ataaaatcgt tatgtaagat
    29941 attaaaaata gaaaagataa gacaattaaa agatgaaata ttattagttc caataacaaa
    30001 gtatagaact aaacagaaaa taggttataa tatagttaca gaattagaag aattattaga
    30061 aaagatgtgc aaggtaaaat aacttgtaaa tttaagaagg gatgttttaa atttgaaaaa
    30121 agtaataaca ttggtaatag ctatgatttt ggtagtatct gtgactgctt gcagttctag
    30181 taaaggagaa actgtggcaa cagttgaagg tacaaagata tcatcagatg aatttaaaaa
    30241 aactatagct ctttataaag attctatgga acaaacttat ggaaaagata tatgggataa
    30301 ggaagttgaa aagggtgtaa aatacaagga caagtttaaa gatttaattc ttgaccaact
    30361 tataactaca gaagtaatat attctcaagc taaaaaagat aacttacttc ctaaaaaaga
    30421 ggatgttgaa aaaagtttta aagaattaaa agacgctatg ggtaaagatg aaaaatataa
    30481 agaacaactt aagaaattag gtatagatga tgagttttta agagaccaac aagagaaaga
    30541 tttagcaatg caaaactatc aaagtaactt tgctaaaaag actaaaatat cagatgaaga
    30601 aatgaaaaaa tactatgata cacataaaga tgaatttaaa aaagatgagg tagaagcttc
    30661 tcatatttta ttgaaaacag tagatgataa taacaagcca ctttcagata aggaaaaagc
    30721 tgaagctaag aaaaaggctg aagaggctct taaagaagtt aaatcaggtg aggattttgc
    30781 aaaagtagca aaaaaatatt cacaagatac ttcagcaagt gatggtggta aattaggatt
    30841 cttttcaagg gggcaaatgg ttgctgaatt tgaagatgct gctttttcta tgaaaaaggg
    30901 tgaagtatct gatttagttg aaactcaata tggataccac ataattaaag tgacagacag
    30961 aataaatgag caaacatcat ttgaagatgc taaagaaact ataaaagacc aactgttgaa
    31021 aaataaatat caagaacaaa tagaaaaatt aactaaagaa gctaaggtag aaaaagatga
    31081 aaaagtaata aataaaatca caatctaaga cttaaaaaaa gagtaatatg cttaaaaaag
    31141 ctatatctgg ataagatata gctttttttg tatatttttc ttaacaatgg taaaaataaa
    31201 cttaaagtaa tttatggagg gattaaaaaa tatgaaagca acaggtatag ttagaagaat
    31261 agatgatctt ggaagggtag ttatcccaaa agagataaga aagacattaa gaattagaga
    31321 aggagaccct ttagagatat tcacagcaaa agatggagaa gtcatactta aaaaatactc
    31381 tccaataggt gaattaaatg aattttcgca agaatatgct gaaacattag ctgaagtact
    31441 aggatatgga gtgttgataa cagacttaga ttctataata gctgtgtcta agcttcctaa
    31501 aaaagattat aaagaaaaaa gtataagtga tgagttagaa gcaataataa gtgaaagaac
    31561 agtaaaatat tcaaaagata aaaacatttt accattattc aaggaagacc aaaaagaata
    31621 tactagtcaa gttatcatgc ctataattag tgcatctggt gactgtatag gttcaattgt
    31681 agttgtttca aaggataaag atacaattgc agaaagtgaa gaaaaatctt taaaaattgc
    31741 tgctaacttt ttaggaaaac aagttcaata attttatcat aaagaccttt tacatgaagg
    31801 tcttttgaag tttttgtgta aaatattgaa tatagcatta aaaatatggt atattaaagt
    31861 ttgttatata aacagtatgg aggttatgtt atgaacaata acaaaaataa agactccttt
    31921 ctaaaaggag cccttatact tgggctagca ggtgtaatcg taaaaataat gggtggattt
    31981 tttaggattc cccttggaaa catgattggt tcagaaggaa tgggatacta tcaggctgct
    32041 tatcctgtgt atactctatt tttaacatta gctactgctg gatttccaac agctgtggca
    32101 aaattggttt cggaaaaagt tgcaattgga aattttaaag gtgcaaataa gatatttaag
    32161 gtatcacata cagttttatt cattaccggt ataatatcat tttgtatatt attttttgga
    32221 gccgactaca tagtaacaaa cgtaatgaaa aatccaggtg cgttatactc tatgaaagcc
    32281 attgctccag cattactatt tgttcctgca atgtctgcat atagaggata ttttcaaggg
    32341 agacaagata tgactaagat tgctgtgtct caagttgcag agcaattttt tagagttgta
    32401 ttaggtctta ctttggccta cttgcttatg aagagtctag gtcagacttt tggtgcagca
    32461 ggtgctatat caggggctac aataggttca atagcatcta tgctttattt ggtgtttgct
    32521 tatatgctag ggagaaaaga gcgtagagca gagatagatg caagccaaag atttaaagat
    32581 gaaagagtgt cttatatttt taaaaagctt ttaactgttg ccattcctat aacaatagga
    32641 gcatcagtaa tgccacttgt aaatatgata gataatgtta ttgtaataag aagacttatg
    32701 gaagcaggat ttacatacaa agtggcaaat tcaatgtttg gtcaattaac aggtatggca
    32761 atggccacaa taaatttacc agcagtcata acaactgcaa tgagtatgag cttagttcca
    32821 gcaatttcta aagcatatgc attaggaaat aagtcaaaag ctagaaaaga tactaagagt
    32881 gcagttaagg taactttact tatagtattg ccttgtgcgt ttggtatggc ttctttagca
    32941 ataccaataa tgggtctttt atttccacat gaaccatctt ctgtaggaac tattttgttt
    33001 actcttactc catgtgtatt atttttagga ttaatacaga cattgactgg tataattcag
    33061 ggaatgggaa aaccaatagt acctgtaatt gctttatgtg ttggtatgct atgtaaaatt
    33121 gtaataagtt atactttaac aggtatacca gatattaatg tactgggttc agcatttggt
    33181 acagttacag catactttgt ggcagccatg attaacttac tatatgttaa aaaacacatg
    33241 aatgtaaact tttctaaaaa agaatttata ataaagccat ttataacagt tatgacaatg
    33301 tttataatgg ttaagttgag ttatggtgct ttagttggat tcttaggaaa cagcatatct
    33361 acaataattg caatatgtgt tggtggaatt gtatatgtag tagtaatact tggaataggt
    33421 ggaattaaaa aagaagagat tctaactatg ccaaaaggtg ataaattata taaattgtta
    33481 aaaaaagtta aacttataag ataatatagg aatatatatg caaataaagg aaatactata
    33541 caaatagtaa atctccttat ttgtataata atataaaaat aaagtgaaat ttgctaatag
    33601 gagtgatgtt atgggtaaga taagtattgt aggtcttgga ccaggagact attctcttat
    33661 aagccaaggt gtgttagaat ctttaataaa tagccctaaa gtttttttta gaacagagaa
    33721 acatccaata attgataaac ttaaagaaac tatacaatac acttctttag actactttta
    33781 tgaaaaagag gaaaattttg ataatgttta tttgaaaata tctgaattta tcatagaaga
    33841 atcagcaaag ggtgacctag tgtatgctgt tcctggtcat ccaagagttg cagagaagac
    33901 tgtaagtata atagagagat tagccaatga aaagcacata gcagtagaaa tattgccttc
    33961 tatgagcttt gtagacgcta tgtttaacta tttggctata gacccatctg agggatttaa
    34021 actggtagat gcttttgaaa tagaaaattc atatatagac ttggatacaa gcatgataat
    34081 aactcaggta tatgataaat ttatagcatc taatgtaaag ttaaaactta tggaacatta
    34141 tgactataat caagaagtat gtatagtaaa tggagcaggt gtcaaagatt tagaaagtaa
    34201 aaagtttata aagctttgtg aattagacag aaatgaaaac ttttttgact atttaactag
    34261 cttatatata cccaaaagtt ctaaaaagat gtataataca gtacatgact tagaaataat
    34321 agttaataca cttagaagtc catctggatg tgaatgggat aaaaaacaaa cacatcaatc
    34381 attaaaaaat tctgttatag aggaagcata tgaactttgt aatgcaattg ataataatga
    34441 tatagatgaa atggtagaag agttaggtga tgttctttta caggttatat tccattgtca
    34501 gataggaaat gaggagggct actttgattt aaaagaagta gtaaatggca tatgtaaaaa
    34561 attaattcat agacatcctc atatatttag tggtgcaaaa ttagatatgt ctaagtttga
    34621 gaaaacttgg gaagaattaa agaaagaaga gaaaggcgaa agtactataa cagatggcct
    34681 tagaaggata ccaaaacatt taccagctct tatgaaggct agtaaagttc aacataaagc
    34741 agcacttgtc ggctttgatt gggacaatgt agaagatgta tttaaaaaaa ttgaagaaga
    34801 atatcaggaa ttacttgacg aacacagggt cgggaatata aaatacataa aggaagagct
    34861 aggcgattta ttattttcga tagtaaatct agctagattt ttagacatag actcaggaga
    34921 agccttgaac tgtactacag aaaaattcat aaatagattt gactttatag aaaaaagtgc
    34981 aatgaacttg aataaaaaat tagaagacat gacacttgaa gagatggatg agttttggaa
    35041 tcaggctaaa aaacaataaa attgtggaag gaaatttgtc aaaaatctag aacttttatc
    35101 acaaagatag aagtctatat tataaacatt tttaggaggt aaatatcgtg aataaagctg
    35161 aattagtatc aaagatggca gaaaaaagtg gattaacaaa gaaggaagca gaagctgcgt
    35221 taaacgcatt tatgagttct gttcaagatg cactagtaaa taatgaaaaa gttcaattag
    35281 ttggatttgg aacatttgag acaagagaaa gagctgctag acaaggaaga aatccaagag
    35341 atccagagca agttatagat ataccagctt ctaaagcacc agttttcaaa gctggaaaag
    35401 gattaaagga tataataaat ggataattag aaatcttgtg aaaaagtgtg gataaatttg
    35461 ccacactttt tctatagaac caaggaaggt gataattatg agattagata aatttttaaa
    35521 agtatctagg attataaaaa gaagaactgt tgctaaagat gcatgtgata aagggattgt
    35581 cactataaat ggaaaagttg ctaaatcttc ttcagaggtg aatattggag atacattaga
    35641 aatacaattt ggagagaaga agatgaagtt taagataaat gaagtaaaag agcatgtatt
    35701 aaaaaatgat gcaaaagaaa tgtacgaaat tttagaataa aattaatagt cctctagtat
    35761 attaatagta gtattattga aatatttact ggggggatat tatggaacag aacataactt
    35821 tgaaagatag atcaaagctg gttatttcgg gagtcgagca catatactct tttaatgaca
    35881 aaagagtaga gctcaaaact tctgtaggcg agatggtaat tgaaggcgaa aacctagata
    35941 tgagcaagct aagtatagac gaaaatataa ttagcataga tggaactata aattctatgg
    36001 tatatgcaaa gcctaaaaag ccacaagaga gtttcttgaa aaaggtattt agatagatga
    36061 taccttttac tcaagatgta agcatttttt atgctactat ttatggtgga atactcatag
    36121 gggtgttgtt tgatttttat agagggctaa gagggaattt taaattcatc aattattttg
    36181 ctataatctt tgatgtattg ttttggtttc tagctacggt tataatattc gtaacaataa
    36241 accttacaga attttttgat ttaagatatt atcattttgt ggctttgttt attggtttta
    36301 ttttatacta taacaccata agtaaaattg ttttatcaat tatcaataaa ataattcgtt
    36361 ttgtgagaaa ttcttttaaa aaagttacac attacatagt cagtttttta aataatttgt
    36421 attacgttat aatctattca ctacacctac tatttgatat tatattttat ataccaaaca
    36481 tatttatagc aacaagaaaa agtataaaaa gaaaatcaaa taaaaaatta aagaacaaga
    36541 aaaaatcaaa acctaagaag aaaaaaaata aaactaaaaa aagggtgtag gaaaaatgaa
    36601 cttaagaaaa aaattttcag gacagattat tgttatcagt ttatttttag gaatatcaat
    36661 ttttagtatg atgacaggat ttgtatttga gtatacaaaa gctaaggaat ataaaaaaga
    36721 aatagcaagt ctaaataaac aattaaaaaa gactgaaata caaataaaca gcttaaagaa
    36781 agatgaaaaa tcatatgaag gagacttaga ggatatagca agaaaaagat tgaatatggt
    36841 aaaaccaaat gaaacagttt atgtagacat aaatcgttga ttaaaacttg ctaataagtt
    36901 tacagatgta ttgattttaa aatttaagat ttaataaaaa agtcattcag ttaaggatgg
    36961 ctttttgttt ttattgagga gggaatttca taatgaaagt aggagctata gatattggga
    37021 caaactcaat gagattactg atagcagatt atagagatgg gaattttata aacagaaaaa
    37081 aatttgttaa tacaactaga atcggttatg gtgtggatag taaaggatat ataaaagaag
    37141 atgctatcaa aagaaatata aaagcattaa aagaattttc tgataagtgt aatgatgaaa
    37201 agtgtgaaaa aatttattgt atggggacat ctgctcttag agacagtaaa aatggagatt
    37261 actttgttaa attagctaaa gaagaaactg gaatagatgt tgatatagtt acgggaatag
    37321 aagaatcaaa tttgggcttt aagggtgttt tagaaggaat acaaggtgat gatactatta
    37381 ttgttataga tattggagga ggttcaacag aatttattgt aggtgattca gaaggaatta
    37441 aatttaataa aagtgagaat gtaggtgctc ttaggatgac agagaggttt ttgaagcacg
    37501 atattattga agatgaagag tttgaagcta tgagttcatt tatatatgat gagattgaca
    37561 aaacgttaaa tcatataaaa gacagaggat taaaaactct tataggtata ggaggtacga
    37621 ttacatcatt atcagcaatg aatcaagaat tagagattta ctctatggaa aaaatacata
    37681 atagtaaaat acacaaaaaa gaaatagaag aaattctaca aaaattaata aagatgacat
    37741 taagtgataa aaagaactta aaagggctac aagaaaaacg tgcggatatt ataactgctg
    37801 gtgtaaaaat attgaatata ataatggaaa aattagaaat agaagaaata accgtaagtg
    37861 aatacgataa tttggaaggc ttaatatgtc aaaagtcaaa aaaaatgtat taatattttt
    37921 tgatttttaa aaccgattat gacaaaaaaa attactcctc tttgttacaa ttcttaatat
    37981 aaacaaaaga ggaggttttt ttatgcaaag aagtgtagca gttgtgaaga ataagactgt
    38041 aaaaccaaat gtcataaaat taataagcgg atatattgat tttactacgg cggcattcgc
    38101 tgtgatggga tttttactta gtaggtctat tatagtagac tctgtagctc ctttaggaat
    38161 tgcctttttt atatgtgcat caaagattga taagtacaag atacctgtat tcatatcaac
    38221 attattagga atattgttat catctaatga ttttggaaat gtagcaaaat atgctgtgtg
    38281 ccttacaata tttatggcta tgaataaaaa attaaaatta tcagattcaa ttgctaaggt
    38341 tgcatttata ggtgcaataa tactacttcc aatctccatg ggacaagctt tcttttcaaa
    38401 taggtatgta tatggtcttc ttatagcagg agtagaagca ataataatgt ttatatctat
    38461 atatgtgttt tcttatggga taggtttaat ggccaatgtc aacaatagga tgtctgtacg
    38521 gacagaagaa gctatatcaa taagtttact gctaaccttt agcataatgg gaataggaga
    38581 tatatctctg tttggaatat caataaggac cattttatcg accgtattga tattgctgac
    38641 ctcaatagta ggtggagcta ccatgggagc atctagtggt gtaattattg gtgtagcatc
    38701 cattttaaat aatatgacat cagctgttta tatggggata tattcttttt caggattaat
    38761 aagtggagca tttaataaaa taaataaata tttctgtata ctaggatata ttctaagttg
    38821 gacaataata tacctatata cttctggtat tacttctaat atgatgcaat taagagatat
    38881 actattaggt tgtttgatag tactagtatt gccagagaga ttatttaata agattgaaaa
    38941 attaataaaa agtaatgtgg cttcaaatga aatcgtatac gattatatta tgagaagcaa
    39001 aaacctaact aatagcagat tgaacagtat ttataaaact tatgatgacc ttgcagatac
    39061 atttgataag ataagagaaa aagacaaagt gctagaccaa agagatatag ctaatgtaat
    39121 agatatgatt cataatgatg aatgtaaaag ttgtagtatg agaagaatgt gctgggagtc
    39181 aagatttaat catacataca ctatgatata taacattctt gaaaaaattg aggagaaagg
    39241 ggagttaagt ctaaacgata tacctaaaaa ttttagaaag gaatgtatga aaccagagtc
    39301 gatagtgaaa atatcaaatc actattacaa aatgtttgta ttagattatg attggagtgt
    39361 gaagttttca gaaagtagaa agttaatagc aaatcagata agaagtattt caaaatccat
    39421 taaatcctta tctcaagatt tagaaggtga cataatgcta gatatagaaa aggaaaaaaa
    39481 tatatatgaa cagttagaaa gatatgatat aacagttgat aaagtaagtt acttaacaaa
    39541 aagtaacaat gagtttgaaa tatcaataga aaagaaaact tgtcatgatg gatgtatgtg
    39601 tgaagataaa attgtaaata taatttcaga cctagtaggt gaaaatatgt ctgttagaaa
    39661 aataggatgt cactgtttag gaggtaaatg caaggcaaca ttcgttaaat ctcaaaaata
    39721 taaggctgtt acagaagttt cagcaatgtc aagggatggg catatccttt gtggagacaa
    39781 ttacacatac atggagataa atgatggtaa atacatgatg gctataagtg atggtatggg
    39841 aaagggtaaa aaagcatatg aagaatcttc tgccactatt gatatactgg aaaaaatgat
    39901 agatgcaaaa attaaagatg agatagtcat tgatactata aataatatgt tgttgttaaa
    39961 atcttcagaa gagatgtttt caactctgga tttgggaatt ttagatttaa aacgaggttg
    40021 tttagaaact ataaagatgg gagcgtgttc tacttatata aaaagagagg atggagaagt
    40081 tgacttaata tcatcatcat cattacctgt gggtatatta tcagatgtaa aaattgatag
    40141 aaaaaatgta aaagttaagg aaggagacta tgttataatg gtttctgatg gaattgtaga
    40201 tgcaggaaga aataacaatt taggagacaa ttggttaata tacttcttaa agaatataga
    40261 gactacaaat ccaaaggaaa tttcaaattt aatattggat agagcattag agttacaagc
    40321 gttacaaatc gaagatgaca tgactgtttt agttacgaaa atatgtacaa aataaggttt
    40381 acaagattat gtgtataaga tgtggataac ttgtgtataa tatgttaata gacttttgaa
    40441 aaaagtatag ttattaacaa aaataacata taaatcaaca aaatttgttg ataagtatgg
    40501 cagaaatgtt aataaaatgt aaaaaaagaa aaaacaaaag cgattatcaa aagagattca
    40561 tgttgaaata actaaatttc taagtaaaga aaacctgtga atatgttgat tattatgtgg
    40621 ataactatta attcgatatt tatttatata gtatgaataa ttgaataata tatctaaaaa
    40681 aagccatcct attattaagt aaatttattg aaaggatggc tttttatgaa tagaaaattg
    40741 tgcaaagcaa taataccttt atttatttct attttttgca tgttaataat aagtataaaa
    40801 gctgaccaag gatatcaaaa gaataatgac agagcaattg ctgtcatgaa taataatgat
    40861 actgaagatg atgctgaaaa aaaacaagaa gtagactatg aaaaaacatg ttggaatttg
    40921 caatctttgt tcaaaagtga tgaccaatgg aaaaaagaac ttaagagttt tgataaagat
    40981 actaaagagt tgaaaaatta cataggtaaa gtaacaaaat cgccaacaca tttgtctttt
    41041 gccttaaaca ttaaggaaaa acttgatata agactaaata aactttctgc ctatgtaaaa
    41101 ttaaaacaag atactaataa aaattcatat aagtacttag atatgagtga tagtatgagt
    41161 aaatcatatg ggaattatat ggggatttgt tcagatttag aattagaaat actaaaatta
    41221 tcaaacaaag attacaagaa aattatttca aataaaaata taaataggaa atatggtgca
    41281 tatttaagag atataagaag gaataaagta cattatttgg atgataaatc agaagatatt
    41341 ctaagcaaag tatcttcaat aagtagtctt ccaagccaag tatatgaatt atttagaaat
    41401 atggatagaa aaactaattt gacaccagcg caatatgcaa gtgaattaga gtcagtagat
    41461 agagaaaata gaaaaaaagc ttatcaagat gaacttataa cttacaatga taatataaat
    41521 acaattgcag gtttaattac tggtcaagtt aaaaagaata ttttttattc tcaggaaaga
    41581 ggttatgagt cttctttaga aatgtacttg gaatctgata atgtagatga aaaagtatac
    41641 aataatttga taaggactgt aaatagtaat atggacagtc tacacaaata tatagattta
    41701 agaaaaaaag tattaaaatt agataaggtt cattcatatg atatgtttgt gcctatggtt
    41761 aaggctgttg ataataatat taattatgaa aaggcacaaa gtatagtata ttcagcgttg
    41821 tcacctttag gaaaagaata caatgatgta gtatataaag ctttcaatga aaaatggata
    41881 gatgtttatt cacatgaaaa taaagtaagt ggtggatact gcttatctgt ttatgaaaat
    41941 catccatata tacttttaaa ttataataat tcattaggtt cagtttcgac tttatcacat
    42001 gagcttggac atgctgtata tgaatactta agttctaaga atcagaatta ttttaattca
    42061 aatccgtcta tatttactca cgaagttgca tctacaacga atgaagcatt actttacgag
    42121 atgcttataa aagaagctaa aggcaatgat gaaaaagctt attatataac acagtattta
    42181 gacttgataa aagatactct ttatacacaa actatgtatg ctgaatttga aaaaactata
    42241 cacgagttag tagaaaaagg aaaaagtgta aacgcattag tattaaatga tgtttgggga
    42301 caacttctta aaaaatatta tggacaaagc tatgaattag accaattatc taaggtcgga
    42361 tgggctagaa tcccacattt ttataatagt ttctatgtct ataaatatgc tacaggttgt
    42421 agtgcaggag taagttttgc ccaagacatc ctaaagaatg gttcagataa ctatatgagc
    42481 tttcttaaaa agggaagttc agattatcca ataaacttgt taaaatcaag tggcataaat
    42541 ttaactaata caaaacccat agaaaataca atcaaaaaat ttgatagttt agtagcagaa
    42601 cttgaaaagc tattataaac attattgtaa tccacatggt aaatatatcg aattaatgat
    42661 aaaatatact aaaagtttaa agtcctatca agataggact ttttatttat taattagtgg
    42721 gagatgattc tatgcaagtt aaagttaaaa aggctgtaat accagcagca ggtttaggaa
    42781 ctagattttt gccagctaca aaggcacaac caaaagagat gttaccaata gttgataagc
    42841 caactctaca atacataata gaagaagctg tggcctcagg aatagaggaa atactaataa
    42901 taacaggtag aaataaaaaa tctattgaag accactttga taaatctgta gaattggaac
    42961 ttgatttaga gaaaaaaggt aagaaagaat tgttggaggt agtacaaaac atatctaaca
    43021 tgattaacat acattatata agacaaaaag agccaaaagg attgggagat gcaatttatt
    43081 gtgcgaggca ttttataggt gatgagccgt ttgcagtaat gttaggggat gatattgtag
    43141 ataatgatgt accttgtcta aaacaactaa cagatgcata tgaggaatat agaactacaa
    43201 tattgggtgt tcaaaaggta aatcaagaag atactaataa atatgggata atagaagcaa
    43261 aaaatataga aggtagagtt tacaaggtaa aagacatggt agaaaagcca gagtcaggaa
    43321 aagctccttc aaatattgct attttaggta gatatataat aactcctgaa atctttgata
    43381 ttcttaagga tttaccacca ggtaaaggtg gagaggtaca acttacagat gctttaaaaa
    43441 tattatctaa gaaagaagct atgtatgcat acaattttga aggcaagaga tatgatgttg
    43501 gggacaagct aggattttta gaagctactg ttgactttgc tctaaagaaa gaagacctta
    43561 aggaagattt tataaaatat ttgaaacatg tatgtagtga atttgataaa aataacaatg
    43621 tgttatacaa taatacagat gaaaaaatag atttaattga agtcaatgaa gctagagaac
    43681 ttgaaattca aaaataatta atgaaaggtg atgtatggtg gcaaaatcaa gtcagaaaag
    43741 aattaataga ataatagctg ttgggattgt gggaatcctc gtaataacga gtataacagc
    43801 tgttttgagt atgattgcaa ctatgtaata agataaatga cagcagttga tatacataaa
    43861 tataaaaacc atatataact tgatgtagtc aaaaacttac aatattttag ttatatatgg
    43921 tttttagaat ttataaatgt aaaaaaaaat ttttataata gttgcatatt ttaaaggaaa
    43981 atattattat aagatataaa tatagtgata aatttataaa aacgtgctaa gattggactt
    44041 gaaatacagt aatatagtag gtaataatgt tttaaatttt atttattgac cataatatat
    44101 aaataatgat attattagac ttaactgttt aaataaaact tttctttttc atgccttcaa
    44161 tttccaaaat aagtatgtat ataaatgaat atatagatga ggaggatttt tttgatttta
    44221 gatgataaag tattagaagg attaggtagt aagaccttag ttgagctacg agagatagct
    44281 aaagaattaa aaataaaatc aattactaca tataagaaaa atgagttaat tgagattata
    44341 aattccaaaa gtaaaaatga ggaaaaagta aacgaagaga ctaaagaaaa aaatgaagat
    44401 attaaagaag acataaagaa tagaaaagaa aatatagagc gtgaaagtat agaaaaagaa
    44461 gttgaatata agagccctac caaatcatac aacaagaaag aaatagatac ccaaaatatt
    44521 tcccaaaacc aagaaaatag aaatcaaaga aatgactata ataagacaac gagtgtaaat
    44581 gatagtaata agtcatcaaa tatatctaat agaatggtca gaaataataa taaaaactat
    44641 tatatgccta aacaagttga tgaatctaag atagtagatg aatttaacac atcaaaagag
    44701 gatgaagtag taggtgtact tgaaattctg ccagatggat ttggtttttt aagaggttca
    44761 aattatttgt ctactgaagg agatgtttat gtttcaccat ctcaaataag aagatttaat
    44821 atgaaaacag gagacaagat taagggaata actagacatc caaaaagtgg agagaaattt
    44881 agagcacttt tatatgttca aaaaataaat gatgaaaacc ctgacacagc tatacaaaga
    44941 aatgcgtttg agacattaac accaatattt ccagaagaac gacttacact ggaaactaat
    45001 agaaatgaaa tagctacaag aattatagat ttaatttcac ctataggaaa gggacaaaga
    45061 ggattgatag tagcacctcc aaaggctggg aaaacagttc ttttaaaaag cgttgcaaat
    45121 agcatagcta aaaatcatcc aaatgttgaa cttatagtac tccttataga tgaaagacca
    45181 gaggaagtta ctgatatgaa agaatctatt gaaggagacg ttatatattc aacttttgac
    45241 caagtatcaa gtcatcatgt caaggttgca gagatggtgt taaatagagc tcaaagatta
    45301 gttgaacatg gtaaagatgt agtaatttta ctagatagta taactagatt ggcaagggca
    45361 tataacctta caatatctcc tacaggtaga actttatctg gaggtattga ccctggtgca
    45421 cttcatggac ctaaaaagtt ctttggtgca gcaaggaata ttagacaggg tggttctttg
    45481 acaatattag gaacagcatt ggttgaaact ggttctagga tggatgatgt aatatttgag
    45541 gaatttaaag gtacaggaaa tatggagcta catttagata gaaaattagc agaaaaaaga
    45601 atattccctg caatagatat ttacaagtct ggtacaagaa gagatgattt attgcttgat
    45661 gatgaagaaa agactgctct ttggagatta agaagagaaa tgagtaataa ttcagtaatg
    45721 gaaattacag ataaagtcat tgaacttata aagagaacta aggacaataa agagtttgta
    45781 aaatcaataa agaatttgta aaatcaataa tacaaatgca aatatggatt atataaacta
    45841 atagaaaaat cacttgaatg gcataataag ttgtgctata ataatgtagt tgataatgta
    45901 aaaagaaagt taatataatt aattttttta aataaagaga agaggtgact ataatgcaaa
    45961 aggaaataca accaaaatac aatccagttg aagtgcgttg tgcttgtggg aacacatttg
    46021 ttgctggttc aactaaggac gaaataaaag ttgaaatatg ttcagagtgc catccattct
    46081 atacaggaaa acaaaagaac atagaaaaag gtggaagaat cgacaaattc aagaaaagat
    46141 tcaaaatgga ctaattttta taaaagtgga gttggaggaa gccaactcct attttattac
    46201 aaaaaaacat tcatattcta ctttaaattg tctttttgta ttataataat aaatatgtaa
    46261 aattgagtat tctaaataca ataaaatgag gtgagtggat ggcaaaacaa gctgtaggtg
    46321 ggcaagctgt tattgaaggt gtaatgatgc agtcaaaaga caaaagagcg gtagctgtta
    46381 gaaaaagtga cggagaaata gcactaaaag aagatagaat aaaaagttgg gtaagagata
    46441 agaatataga taagatacct tttgttagag gttcttttgt tatgatagat acaatgattc
    46501 aagggataaa aagtttaaac ttttcttcag agttttttat ggaagaagct gaagaggata
    46561 aatttgattt atttataaag aaaattttta aagataaggc aaatgatata attataatat
    46621 tttcattggt catagcaatg ttactttctg ctggattatt catttttata cctacattgg
    46681 taggaggagc tttttctaaa gtaatgccta atgattttat gcttaattta atagaaggaa
    46741 tcataaggat agctatatta tttgcatata tagtattgat ttcacgaagt aaggatatag
    46801 aaagagtctt tcaatatcat ggagcagaac ataaatctat atattgctat gaaaatgatt
    46861 tagaacttac agtagagaat gctagaaaat ttaaaagatt acatcctaga tgtggtacaa
    46921 atttcctatt tatagttatg gctgtatcaa taatattgtt tgcatttttt ggttggccaa
    46981 atccaattct tagaatattt atgagaatta tatgtgtgcc tatagtagca ggtttatctt
    47041 atgaggtaat tagagttctt gggaaatatg ataatggatt tactaaaatt attgcatatc
    47101 caggaatgat gttgcaatat tttacaacaa aagagcctga tgatgagcaa ttagaggttg
    47161 ctttggaagc attgaaagca gtagtagatt agagaaaagg atatttgtta tgacgataaa
    47221 agatataatt attaaatatt cagataaact aaaagatata agtgatactc caagattaga
    47281 tacagagtta ttgttacaaa aaacattggg tgttgataga ttatatattc acttaaatct
    47341 gaataaagag ctaactgaag aacaaaaaac aaaatttatg ggttttgcag aagaaagatt
    47401 aaatggaaga ccaatagcat atatagttga aaatagggag tttatgggat tagatttttt
    47461 tgttaaggaa ggcgtattga ttccaagacc agatacagaa actttagtag aagaaataat
    47521 tgaaatatgt agagaaaaaa aagatgtaag tatattagat ataggaactg gttctggagc
    47581 tattacaata agtttagcaa agtacattga aaattctaaa atcatgtctt ttgatatatc
    47641 agagacagca ttagaaatag ctaaaaaaaa tgctattata aatgaagttg gtgaaaaaat
    47701 aaagtatatt aattctgatt tatttacagc aataagtgat agtaacataa aattcgatat
    47761 catagtttca aatccaccat atattaaaaa acaagatata gaaactctgc acaaacaagt
    47821 taaagattat gaaccatata atgctcttga aggtggagaa gatggattag acttttatag
    47881 aagaataaca gaacaaggta agaaatattt aaataagtgt ggaatattag catatgaagt
    47941 tggtcataat caagctgaag atgtaataaa tattatgaaa agtaatggat ataaaaaaat
    48001 atacacaaag aaggatattc aaggcattga tagagttgtt ataggttaca atatatgata
    48061 taatcaacaa ttgatatgtt acatttaatc gaggtgaaag tatgttaaaa aaattagagg
    48121 tattggaaga tacatataaa gacttaagtg aaaaaatagg tgatccggat gtaataaatg
    48181 atcaaaaagt ttggcagaaa tatataaaag aacatgctga cttggaacct attgttatga
    48241 aatacagaga gtataaaagc gttttagata gtataaaaga atcaaaagaa attttacaag
    48301 aagagtcaga tgaagaatta agagaattag ctaaaatgga gttagctgag atggaagaaa
    48361 aagtagcacc tctggaggaa gaaataaaaa tactattatt gcctaaagac cctaatgatg
    48421 ataagaacgt tatagttgaa attagaggtg gagctggagg agatgaagca gctctatttg
    48481 cgggtgattt atttagaatg tactcaaggt atgctgaaag aagaagatgg aaaatagaat
    48541 tattgagtgc aagtgatact ggagttggtg gatataaaga agtatctttt atgataaaag
    48601 gtaaaggtgc ttattcaaga ttaaagtatg aatctggagt tcatagagtt caaagaatac
    48661 catcaactga atctggtgga agaatacaca catcaacttc tactgttgca gtattaccag
    48721 aagttgaaga tgtagaggtt gaaataaatc caaatgattt aagaatagat gttttccgtt
    48781 cttcgggtaa tggaggacaa agtgtcaata ctactgactc tgctgttagg gtaacacata
    48841 tacctacagg tgaagtagta tcttgtcaag atggaaaatc acaattaaaa aataaagagc
    48901 aagctttaaa aatattaaaa gctagacttt acgataaagc attggcagaa caacacaaag
    48961 atatagcagc agaaagaaaa agccaagtcg gaacaggaga caggtcagaa agaataagaa
    49021 catataattt ccctcaaggt agaataagtg accatagaat aaatttaact ttatataagt
    49081 tagatgcatt tttagatgga gatatagatg aaatgataga tgctttaatt actgttgacc
    49141 aaacagaaaa aatgactgca atataagtaa tgttttaaag aagctgtttc ttcctagaaa
    49201 ttattttcta ttttgaaaca gctattttgc attttaggtc atgttatcat attctattgt
    49261 atataattgt tatatacaag tataggaggg ttatatgata ctaaaagtaa caataattgg
    49321 gcttttggca ggtgttatag ggacaggatt aggaggagta atatcagcca tttttaaaag
    49381 agaggtcgat aaatatctaa gtttttttat gggattatct ggtggaataa tgttggctgt
    49441 agtggtgttt gatttaatga aagaatccat ggacaaaatg gggataatca atacagttat
    49501 atttacgttt gttggagctc tcataactat gtacataaaa acaaaattag atgtgtctgg
    49561 aaatatggca tctggatatc ttatattcat aagtatatta ctacataatt taccagaagg
    49621 actagccatt ggttcttctt ttatgtcaac tgaaagttta ggtattacac ttgctatagt
    49681 tataggtcta cataatatac cagaagggtt agctatggca ttaggcttag tttgcaataa
    49741 aatgaaacta agtaaagtta ttttgtttac tgtaatagct ggattaccta tgggtctagg
    49801 aagtttttta ggtgtttatt ttggaggagt atttacttct cttataggag tatttcttgc
    49861 tacagcagga ggtactatga tgtatgttgt attggaagaa atttttccac attcaaaaag
    49921 tgtgtattgt ataattggtt tcttgttagg tacaatgatt gtaaattata tataaaatta
    49981 aaatgaaagc aggcatacaa aagatgaaaa ctattataag taatattgat ataaataata
    50041 ttgattatga ggaaataaaa atacaagcaa aacttcttag agaaggtaaa acagttattt
    50101 ttccaactga gacagtatat gggttgggtg caaatgcatt agatgaaaat gcagtaaaga
    50161 agatttatga agctaaagga agacctagtg acaatccatt gatagtccat atatatgaaa
    50221 aagaagaagt atatgactta gccaaagata taagtgataa agctaaactt gttatagaaa
    50281 agctttggcc aggacctata acaattatat taaataaaaa agatataatt ccatacaaaa
    50341 ctagtggagg gcttgataca gttgcaataa ggatgccttc taatgttatt gccagggcaa
    50401 taataaaaga agctggtata cctattgcag ctccttctgc aaatatctca ggtcgcccat
    50461 ctccaacaaa agcaaaacat gtatatgaag agatgaatgg aagagtggat ggaatagttt
    50521 taggaggaga cagtaatttt ggattggagt caactgtatt ggatttaaca gaagagactc
    50581 ctatgatact gagaccagga agtataacta aagaagtttt agaggaactt cttggagaag
    50641 taaagcttga cccatcatta agtaaaaaag aagataatca aaaagctaaa gctcctggaa
    50701 tgaaatataa acattattct ccaaatgcag atgtatatat aatatctgga aaaagagaaa
    50761 atgtagcaac aaagattaat gatatgataa aatctaatag agacaaaggt ctaaagacag
    50821 gtgtaatgtg tatcagtaga aaccgagagt tttatgatgg agaagtaatc gatttaggaa
    50881 actctcttga agaagttgca agtaatctgt ttgatgctct tatagaaatg gacaaaaagg
    50941 gcgttgatgt catatattca gaagagttcc ctaagactgg agttggacaa gcaattatga
    51001 atagactact gaagtcagca ggatacaaag tgataaaggc ttaagaggga gttgtaaaat
    51061 atgaatatac ttattgtgtg tacaggtaat acttgtagaa gccctatggc tgaggctata
    51121 ctaaggaaag caattaaaga atctggaaga agtattgaag aatattcgat atcatcagca
    51181 ggtatatcta cagcaaatgg aatgggtgct tctgaaaatt ctatagaagt actaaaagaa
    51241 atcggtatag acttatcaaa tcatagaagt aaagtaatta ctaaaaaatt aattgatgaa
    51301 tcagatataa tactaacaat gactaaatct cataaagaaa ttttagtaca agcagttcca
    51361 aaatgcaaag aaaaagtgta tacttttaaa gggtttgcaa ataaaaatga ggaagatata
    51421 tctgacccat ttggtggaaa tttggatatt tatagaagca ctatgagaga aataatgtat
    51481 tctgtcaacg aaatagtaaa aaaaatatga aacggaggat aaacaaatga agataggatt
    51541 aggctgtgac catggaggat ataatttaaa aaaagaaata ataagctatt tagaaagtaa
    51601 aggtatagaa tgtgtagatt atggaactaa taatgctaca gattctgttg attatcctgt
    51661 ttatggagaa atagttgcaa attcagttat aaataaagaa gtagattatg gaatattatg
    51721 ttgtggtaca gggataggaa tatctttagc tgctaataag gttcctggga taagatgtgc
    51781 agttgtatca gatgtatttt ctgcaaaaat gtcaaaagca cataatgatg caaatatgtt
    51841 atcattaggc gaaagagttt taggaaaagg tctagcactt gagatagttg aagcatggat
    51901 taatacggat tttgaaggtg atagacatgc tagaagagta aacatgatta aatcaataga
    51961 agaaaaacat aataaataat aaaaattaca gttttatcag gaggaattat caaaatgagc
    52021 aaagtggtag agacaaacca tccattaata caacataaat taactttaat gagagataaa
    52081 aatactggtt ctaaagactt tagagagctt ttaactgaaa ttgcaatgtt aatggggtat
    52141 gaaataacaa aagatatacc attaaaagat gtagaaatag aaactcctat tcaaaaaaca
    52201 tcatctaaag tagttgcagg taaaaaatta gcaataattc caattttaag agcaggtctt
    52261 ggaatggtag atggattagt aagcttaatg ccagcagcaa aagttggaca tgtaggacta
    52321 tatagagacc cagaaacatt aaaaccagtt gaatattatt gcaagcttcc tcaagacata
    52381 ggagaaagag atataatagt agttgaccct atgcttgcaa cagggggttc tgctgtagca
    52441 gctatagatt tacttaagtc taaaggagct aaaagtataa aattagctaa tttagtagct
    52501 gcaccagaag gtatagcaga agtgcaaaaa tatcatgatg atgttgatat atatgttgca
    52561 tcagttgacg aaagattaaa tgaacatgga tatataattc ctggtcttgg tgatgctgga
    52621 gatagattgt ttggaactaa ataaaaataa ataactataa atatttatta gggactttct
    52681 tatatagtgt atataatttt atgtaagaag gttctttttt atatattttt tctcaaaata
    52741 ggaattatga attgataaat ttctcgaatt gagaataaaa agtttttacc aattaaaaaa
    52801 gattgcttat tttacaaggt tttatagaat agtagtctat tttaatgtgg catgttagtt
    52861 gcttacgatt aagattatag gtattaagtg tattagttaa attgagaatt gaatattaat
    52921 aatattattg ggagtggtaa tacatgaaga attgtatagc agtaagaggg gctggagatt
    52981 tagcaacagc agttattcat aaattacaca acagtggttt taaagtagtt gcgttagaaa
    53041 tagaagagcc attagctata agaaggcaaa tttctttttg tgaggctgtt tatgaaaaaa
    53101 aagtaactgt tgaaggtgta gaatgtatat tttgcaatac tgaaaaagaa atagaagaag
    53161 ttttatctca acataaagta gccttgatta tagacccaaa aggagaaaaa ttatctaagt
    53221 taaatccaat cgttttggtt gatggaatac tcgctaagaa aaacttaggt acaactaaag
    53281 atatggcacc acttacaata gctttaggtc ctggattttg tgcaggagaa gatgtagatg
    53341 ttgttataga gacaatgaga ggtcataatc taggaagaat aataactaat ggtagagcag
    53401 aaaaaaatac aggagtacca ggagcaataa aaggtttttc taaagagaga gttatgcatt
    53461 cacagtatga tggtataatg gaaaatgtat gtaagatagg tgatatagtt gaaaagggag
    53521 acctactttg ttatattaat aattatgaaa aaaaacatga agttagagca acaatatctg
    53581 gagtccttag aggtttatta aaggatggag caaaggtgaa caaaaattta aaaatactag
    53641 acatagaccc tagaaaagaa gaagttaaga atgcttttac aatatcagat aaagctagat
    53701 gtatagctgg aggggtttta gaagccatac taaacaatgg gatttatccc caaaattaat
    53761 ttatattaga taaagctatt aatattttat taagatgata taataaattt aatagattaa
    53821 agagagcata tgacgggtat ttaaaattta aatggtaaaa atgctctttt tttattggac
    53881 attcaattaa catgtgtata taatataaaa ataagtattt atttaggttg aaatctaata
    53941 aatatataat accagtacat aagcaaagag gagaaaagta aattatgaga ccatcatggg
    54001 atgaatactt catggaaatt gcagaagtag tgaaaaagcg ttcaacttgt attagaagac
    54061 aagtaggagc tgtaattgta agagacaaac aaatattaac aactggatat aatggttctc
    54121 cgagaaatct tgaacactgt gaaaatattg gatgtaagag gcaggaatta aatattccct
    54181 caggagaaag gcatgaatta tgcagagcat tacatgctga acaaaatgcg ataatacaag
    54241 ctgctcataa tggtattagt gtagatgggg caactttgta tgttactaca agaccttgtg
    54301 ttttgtgtgc aaaaatgtgc ataaatgcag gtatattgaa aattgtttat gaaggtgatt
    54361 atccagatga tatgtctaca gaattattaa aagaagcagg aatagaactc ataaaatttt
    54421 agtggataaa atttatatga caataaatgt caaattatga cactgaaata caatggtatc
    54481 aaatgattaa aagaaagtat ttgaaaaatc atcagattag tagtaagata tattttataa
    54541 ttaaataaat ttatgagata gattaattac atgttattta ttttataata atgagggata
    54601 ctatttatga gtaaaagaaa tacacataca gaaattgcaa agttgtttag tttaataagt
    54661 caaataggat tgatgattgt aatatcaata ttaggatgta ctttcttagg aaaattttta
    54721 gatagtaaat tgaatactac tccagttctg acaataatat ttttattgtt gggtgttgga
    54781 ggtgctttta gtggtgttta taaaactcta atagtatata cacgaaggaa gtgatatatt
    54841 ggatataaaa ttacttaacc aggtaaggtc agtaacaaaa ggtatagtaa tatttgatgt
    54901 agttttaatt gtgctaatgt tgataacatc aacattaagt aaaccagtat tattaggact
    54961 tatatttggc tctataatag ctatgttaaa ttttagatta ttggcgatat cactggaaaa
    55021 atcagtgaca tttcctgctg ggaaggcaca agcttatgca tcagcacaat atgctattag
    55081 attatttata atggcagttg ttctaattgc atcagctaca gtaccacatt taaatataat
    55141 cggaacagca ttgggattat tgagcacaaa gtttgtcata ttatcaaaaa actttataaa
    55201 taaattaaag aggaaggagg cataagtatg aatcaagcgc attattatct cataggaaac
    55261 tttaagatta gtgaaacaat actagtaagt tggggaataa tggccttttt agcgatagca
    55321 tcttattttt tgactagaaa cttaaaaaaa gttccaacta gtaaagttca aatattctta
    55381 gaatttgcag taggtggtct tgctaaaatg gttacagatg ttatgggtaa aaatacagta
    55441 aaaagaatgc catatatgat accatacata ggaagcttgt ttttattttt cgcatgttca
    55501 aatttaagcc cattacttgg attaaggtca ccaacaaccg acttagacac aactgctgca
    55561 tgggcaatga ttacattttt tatgatttat tttgcaggag ttaaatttaa ggggttatca
    55621 tactttaaag aattggctca accacttgcc ataatgttac caatcaatat tataggtgag
    55681 ttagctagac caatatcatt aagtttccgt ccatttggga atattttagg tggaacaata
    55741 ataatgggtc tgttttatca acttttaggt tttatatcta gtttaatacc aggattatca
    55801 ataccaattg gagaaatatt aataccagtc ccattacact tgtactttga cttatttgca
    55861 ggattgttac aagcatatat atttataatg ttaacaatgg tatttatatc aagtgcagga
    55921 gagttagatt aagaaatcaa cttatattaa gataaaaata aaataattta aaaaattaaa
    55981 gttttaagga ggaaataatt atggaaacag caatagtagc ggcagcatca gcaataggag
    56041 cagggatagc agttgcaaca ggtataggtg caggtatagg tcaaggtata gcagcagcta
    56101 aagctgcaga agcagtagga aaccaaccag aagctaaagg tgatataaca tcaactttat
    56161 tactaggagt tgctatagca gagtcttcag caatatatgg tttggttata tcaataatac
    56221 ttctattcgt aaatccattc tttaaatact taggaatgta gatttagtcc ttaaatattt
    56281 tgagataggt ttataggtaa atcatctcaa acttataatc atgagatgaa ctttatcatc
    56341 tcgtgattat aagtagttat gatttatgga aaggaggata attatggaaa aagcactagt
    56401 aggtataacg tgggaatttg tgttccaaat tgttaatact tttataatat ttttacttct
    56461 gagaaagctt ttattcaagc cagtattaaa tataattgaa tcaagagaaa atgatattaa
    56521 aagtgatttg gcagaaggcg aaaaagcaaa aaatgaaggt ttagctctga aaaaagaata
    56581 tgagtcaaaa atcaattttg ccaaggatga aggtcaagaa atcataaaac aagcaactat
    56641 aagagcagag cagaaatcag atgatatagt aaatactgca aagaaagatg ctcttgatat
    56701 aaaagaaaaa gcaaacaaag atatagaaca agaaagacaa aaggttataa atgaaatcaa
    56761 aaatgatata tctaacatag cactacttgc agcatctaaa gttattgaaa aagatttaga
    56821 taaatctaaa catgaagaat tgatagaaaa ctttataaaa gaggtagggg aagctaaatg
    56881 ataaatgtaa tagcaaatag atatgccgaa gccctatttc aattaggaga agaagaaaat
    56941 tcaactgatg tcttattcaa agaactagaa aaagtagtag atatgatgac taaagttagt
    57001 aaagatttct acaaagtttt aaaatcacca ttagtttcaa aatcagaaaa gaaaaacttg
    57061 gtagaaatta tttttagtaa ggaagtaagt tctaacataa aaaacttttt aaaagtttta
    57121 gtagataaag atagaatatc atatttagaa gatatcgaat tagcttataa agaattatta
    57181 aataagaaaa acaatgtaat tgatggagta gcaataagtg ctattccaat gagtgaaaca
    57241 gatattaaag aacttgaagt caaactttca aataaataca acaaaaatgt tactatagaa
    57301 aatgttgttg acaagactat actaggggga gttctagtta gaattggaaa tgaacaaata
    57361 gatggcactg tgaaaactcg tcttgataag atgaaagaaa agctatctga agtaatatct
    57421 taagaatggc ggtgaaccca tgaacttaaa acctgaagaa ataagttcta taattaaaca
    57481 gcaaataaaa aattatgaga ataaagttga gttgacagat acaggtagtg ttttaacagt
    57541 tggggatggt atagcgagtg tatatggatt agaaaaagct atgtctggtg agttgctaga
    57601 gttcccaggt gaaatatatg gtatggcact taacttggaa gaagaagtag ttggagctgt
    57661 tatccttgga gatgacagtg aaataaaaga aggcgatata gttaagagaa ctggaagaat
    57721 agttgaagtt ccagttggag aagctttaat aggtagagtt gtaaactcac taggtcagcc
    57781 aatagatgga aaaggaccta tagcgtacac aaaaactaga ccagttgaat ctgaggcacc
    57841 tggtataata gatagaaggt ctgtttatga accattacaa acaggaatca aatcaataga
    57901 ttcaatgata ccaattggta gaggtcaaag agagttaata ataggtgaca gacaaactgg
    57961 taaaacatct atagttattg acactatact aaatcaaaaa ggaaaagatg ttatctgtat
    58021 atatgtagca attggacaaa aacgttctac tatagctcaa ttagttagct ctttagaaaa
    58081 aggtggagct ttggattata caatagtagt ttcagcaaca gcatctgaat cagcaccact
    58141 tcaatatata gcaccatatg ctggagcagc tatgggtgaa gaatttatgt acaatgggaa
    58201 acatgtactt atagtgtatg atgatttaag taaacaagct gttgcatata gagaaatgtc
    58261 attgttactt agaagaccac caggacgtga ggcatatcct ggagatgtat tctatttaca
    58321 ttcaagatta cttgaaagag cagctaagtt atctgatgaa ttgggtggag gttcaatgac
    58381 tgccttgcct ataatagaaa ctcaagctgg tgacgtttct gcatatatac caacaaatgt
    58441 tatatctata acagatggtc aaatatactt acaaccagag ttattctatt caggtgtaag
    58501 accagcagtt gaccctggta tatcagtatc aagggttggt ggttctgcgc aaattaaagc
    58561 aatgaaaaaa gttgcaggaa cattaaaact tgcatattca caatatagag aacttgcagc
    58621 attctcacag tttggttctg acttagatga agatactaaa aagagacttg ctcaaggtga
    58681 aagaatcgtt gagatattaa aacaaggtga gcatcaacca ataaaagttg aaaatcaagt
    58741 tatgataata tatgctgtta taaataatca tttagaggat attccgatag ataatatagc
    58801 tagatttgaa tcagaattgt atgcatttgt agataataat tatccggaaa tatcaagaaa
    58861 aatattaggt ggagaagact tcactcatga tttaacagat gcaataaatg aatttaaaga
    58921 aaagtttgtt gttgaagtat aatccttttt aaaaaaggag gtaaattaat tggcaggagc
    58981 tggaatcaaa gcaatcaaaa cacgtatggg aactgttgaa agtacaaagc aaataactaa
    59041 agcaatggaa ttagttgcat cttctaagct tagaaaagca aaagaaaagg ctgaagattc
    59101 aagagaatat tttaatactt tatacaatac agtaaaagat attgcccaaa caacacgtgg
    59161 aattagaaat gaatttttaa aagaaagaga agtaaaaaat aaatgttatg tagtagtagc
    59221 tggagacaga ggacttgcag gtggatacaa ttccaatgta ttaaaggaat ctgtctctca
    59281 tatgaaaggt aaaaaggaaa cagtgattac tgttggaaag aaagcacatg aatttttttc
    59341 taaaagaaac tacaatatag caaaagatat aaatagtgtt gaagcttgtg gatatgaaga
    59401 tgcgacagaa atttcacaaa ctattattga cctgtataaa aaaggtgaag tagatgaagt
    59461 ttatattgct tatacaaaat ttaaatctgc tttatcacaa gaagttgaaa ttttgaaatt
    59521 gcttccatta gtctttgaaa aagatacaga cgaacaagga atggcagtaa aagaagaaaa
    59581 acaaaataga tcaaaagtac aatatatacc atcagcagaa gcagttttaa gttatattgt
    59641 acctcagttt atatcaggta cagtatatgg tggaattttg gaatcttttg catcagagca
    59701 aggtgctaga agaactgcaa tggaatcagc cacagacaat gcgaatgaaa tgctatctaa
    59761 tttagagtta agttataaca gagcaagaca gtcagctgta actcaagaaa taacagagat
    59821 agttggtgga gttgaagctc taaaataggg aggttaggaa atggcaaatg taggtaaagt
    59881 agtacagatt gtaggagctg tactagatgt taagtttgat agtgaacaaa gcctacctaa
    59941 cttattaaat gcgttagtaa taaagttagg agataaagaa atagttgcag aagttgctca
    60001 acatataggt gatgatactg taagatgtat agctatgagt gcaacggacg gacttgtaag
    60061 aggtatggaa gttgtagata caggagggcc aataagtgtc cctgttggag atgagacttt
    60121 aggtagaata ttcaatgttc ttggaaaacc agttgatggg aaaccagctc caaaatctgc
    60181 acctaaatta cctatacata gaccagcacc ggcgtatgat gaactagaaa ctacagcaga
    60241 gatactagaa actggtatta aggtagttga cttactagct ccatatctaa aaggtggaaa
    60301 aataggtcta tttggaggag caggagtagg aaagacagtt ctaatacaag agcttataaa
    60361 caatattgct aagcaacatg gtggtatttc tgtattttca ggagtaggag aaagaacaag
    60421 agaaggtaac gacctttatg gcgaaatgag tgagtctgga gttataaata aaacagctct
    60481 agtatttggt caaatgaatg aaccacctgg agcgagaatg agagttgctt taactggact
    60541 tacaatggca gaacatttta gagatgagca aggacaagac gttttacttt tcgttgataa
    60601 tatattccgt ttcacacaag ctggttcaga agtttcagca cttctaggac gtatgccatc
    60661 agctgttggt tatcagccaa cattagctac tgaaatgggt gcacttcaag agagaataac
    60721 atcaactaag aaaggttcaa taacatctgt tcaagcagta tatgtaccag cagatgactt
    60781 aactgaccca gcaccagcta caacattctc tcacttagat gcaaaaacag ttttatcaag
    60841 acaaatatca tcactgggta tatatccagc agttgaccct cttgaatcta catcaaggat
    60901 tcttgaccct agtatagtag gaaaagaaca ttatgaagta gctagaggtg tacaatcaat
    60961 acttcaaaga tataaagaac ttcaagatat aattgctata cttggtatgg atgaattatc
    61021 tgatgaagat aagttaatag ttgcacgtgc tagaaagata caaagattct tatctcaatc
    61081 attcacagtt gctgagcaat ttacaggaaa tccaggtcaa tatgttcctg ttaaagagac
    61141 tgtaagagga tttaaagaaa ttcttgaagg gaaacatgat gacttaccag agtcagcatt
    61201 cttatttgta ggaacaatag aagacgctgt tagaaaagcg aagggaagta tgtagtcatg
    61261 gccagtgaat tttcattgaa agttgtaact ccaagtaata ctttttatga tggagatgtt
    61321 gaaatgatag tagttagaac tacaaaagga gacaaagggg tgttgaaagg tcatatacca
    61381 tttgtttcag gtatacctgt aggaacttta aagataaaaa aagagggaag ttttaaagag
    61441 gcgaaaatag cagaaggatt cattaatgta actcaagata aaactgtcat attgacagaa
    61501 tctgctgagt ggatttaata aattaattta gagtataaaa agtcattttg accaaaaaaa
    61561 cttttgggta aaatggcttt tttgtgtttt ggtgttaata aaataacgat aaaattgtaa
    61621 gtttttagtg ataataaatt cacaaaatgt ttaaatttac tgccaaatta tgttgtaatt
    61681 aataagataa agaagattta gatagctttt atgtaagtga aaaagtcttc atataagaaa
    61741 tacatataaa atttttataa aatttaaaaa atttgcttag ttgtgtagta taataaataa
    61801 tataatagat ttgtatataa tattcattac aaataatttt aaaagttaca gttttattat
    61861 aatgattatt aaattaagat aagtaacata aactaaataa taatgtgtat tatcgatagt
    61921 aaatttataa attcattatt aattattgaa agaagtatta taaattttct ggaggaacaa
    61981 tgaatatatt tgatattggc gtagatataa ttgaaattga tagaataaga aaggctgtag
    62041 acaaaaataa taggtttttg gagaagatat ttacagatag agaaatagag tattttaatt
    62101 ctaagaattt taaagcagaa agtatagcag gtaatttcgc agccaaggaa gctataagta
    62161 aatctatagg tacaggaatt agattgttta attttaaaga tatagaagtt ctcagaaatg
    62221 aaatgggaaa accaatagta aaaacttata ataatctagc aaaaatgtgc atagattata
    62281 atgttttaga aataaaagtt tcaatatcac attcaaaaga ttatgctata gcaaatgcga
    62341 taaccataat taaggactaa ggagtgatta cattatgatg gataaaacag caaaagaaat
    62401 aatgacaaca gatgtaatag tagcaaaaca agatgatagt atagcagatg tagctaatat
    62461 gctaattgca gaaaaaatag gaggtctgcc agttgtagat tcagagaata gagttgttgg
    62521 aataatttct gaaacagata ttcttaaaaa agaaaaatat atagaagcac ctctctatat
    62581 aaatctatta caaggattga ttttcttaga tgacttaaaa aaagttgaaa aagatataaa
    62641 acaagtagct gcttataagg ttggagaact tatgtctaaa gatataataa aggttcatga
    62701 agatgataag tttgatgatg tagctaatgt aatgattaaa aaatcaataa acagagttcc
    62761 tgtagtagat gatgacaaca aacttaaagg tataatttgt agatatgata taataaaagc
    62821 tttatacaat gaataaactg aagtaggagg tgtgatgtga gaaaaaagtg gaccatagta
    62881 tgtattatgt tcttggcact ggttataatt gtaataggat gccaaaaacg acagtccaca
    62941 aaagaagaag tatacaaaga ttttcaaaag cagatttctg atatgaatta ttacagttgt
    63001 aaagctgaag tagaagtagt tggaaataaa agcccacaca attatgtttt aattcatact
    63061 tataaaaaaa ccgataacta taaattagaa gtaatttccc caaaacattt aaaagggaaa
    63121 agtatagaat atcaaggtga taaaatatta gttaaaaatc caaagattag tgatgtagtg
    63181 gagttaccta atacaggaaa aaataatcaa tatctttttg taggagactt tataaaaaac
    63241 tatcttcaaa atgaggagat gaaagtaaag ttatccaaag gacatttggt tttagagact
    63301 tttattcctg gggataataa atattttaac aagcaagtat tatatgtaaa tgctgacaca
    63361 aaaaatcctg aaaaaatgga agtgttagat aaagagggag tgccaagatt tacagtaaaa
    63421 tacaaagatt ttgaatacag aaactaaggg ggactaaaga catgcaaaaa ataacagtgc
    63481 ctacatgggc agagataaat ctagataact taagatttaa cttaaataat attaaaaatt
    63541 tattagaaga agatattaag atttgtggag taataaaagc tgatgcatat ggacatggtg
    63601 cagtagaagt tgcaaaattg ctagaaaaag aaaaagtaga ttacttagca gtagcaagaa
    63661 ctgctgaagg aattgaactt agacaaaatg gcataacact tcctattttg aacttgggat
    63721 atactccaga cgaagctttt gaagattcta taaaaaataa aataactatg acagtttatt
    63781 ctttagaaac agcacaaaag ataaatgaaa ttgcaaaatc tttaggagaa aaagcctgtg
    63841 ttcatgttaa aatagactca gggatgacta gaataggttt ccaacctaat gaggagtcag
    63901 tacaggaaat aatagaatta aataaattag aatatatcga tttagaaggt atgtttactc
    63961 attttgctac agctgatgaa gtaagtaaag agtacactta taaacaagct aataattata
    64021 aatttatgtc tgataaatta gatgaggctg gtgtaaaaat agctataaaa catgtatcaa
    64081 acagtgcagc tattatggat tgccctgatt taagattaaa tatggtaaga gcaggaataa
    64141 tattatatgg tcattatcca tctgatgatg tatttaaaga tagattagaa ttaagaccag
    64201 ccatgaaatt aaaatcaaaa atcggacata taaaacaagt tgaaccaggt gtaggaataa
    64261 gttatggact aaaatacaca actacaggta aagaaacaat agctacagtt ccaataggat
    64321 acgcagatgg atttactaga atccaaaaaa atccaaaggt tcttattaag ggagaagtgt
    64381 ttgatgtagt tggtagaata tgtatggatc aaataatggt tagaattgac aaagatatag
    64441 acataaaagt tggagatgag gttatactat ttggagaagg cgaagttaca gctgagcgta
    64501 tagctaaaga cttaggaact ataaactatg aagtgttatg tatgatatca agaagagttg
    64561 accgtgttta tatggaaaat aatgagcttg tacaaataaa cagttatttg ctaaaataaa
    64621 accatgatat tataatctgg gaggcgattt tgtgtacgat aagagtaaag aaaaaataga
    64681 agttacttta cctgactctc tgatttctga agttgatagt atagttcaga tggagaacag
    64741 taacagagaa gattttgcaa aagctgcttt tcagttctat ataactcaga aaaagaaaat
    64801 tcatattaaa gaatctatga aaacgggtta tagagaaatg ggtcaaatta atctgtcact
    64861 agctgaatta ggtatgacag ttgaagaagt atcttctgat gataaaaatg aagggaagat
    64921 tgcccaaggt gagtactaat tgtgaaataa agcgtggtga tttatattac gctgatttaa
    64981 gtccagttgt tggttcagag cagggaggcg ttagacctgt tctgataata caaaatgatg
    65041 taggtaataa atatagtcct acagttatag tatcagctat aacatctcag attaataaag
    65101 ccaaattacc tactcatgta gagataagtt ccaatgaata tgggcttaat aaagactcag
    65161 taattctttt agagcaaatt agaactatcg ataaaaagag actgagggaa aaaattggtt
    65221 gtctagatga aaatatgatg ataaaagtag atgatggact tcaaataagt ttaggattgt
    65281 ttacttttta aagagggatt tgtcttttat tagacaatcc cttttttctt ttaaatatct
    65341 cttaaggttt aattttaatg tactatgtta ataaaattaa aaataacgca aaaaaatagc
    65401 ataaaagtga tataataata tggaattgat ataaaattac gaactcaagg gaggtaattg
    65461 catgagagac cataaaggat taaatgagat tgcgcgtatc ataagaagag atatagtttc
    65521 aatgatacat agagccaaat ctggacatcc aggagggtca ttatcagtag ttgaaatact
    65581 tactgcgtta tactttgatg aaatgaatgt ggattcttca aatcctaaaa tggaggatag
    65641 agatagattt gtattatcaa aaggacatgc tgcaccagca ttatatgcta cattggctga
    65701 aaaaggctat tttgataagg aagaactgaa tggattaagg aagataggaa ggatgttaca
    65761 aggtcatcca gacatgaaag gcacgccagg tgtggaaata tctactggtt ctttaggtca
    65821 aggtttttca gtagcatgtg gtatggctat ggcatcaaag ttggataatg ctccgtggaa
    65881 tgtgtatact ttgcttggtg atggtgaggt tcaagaagga attgtttggg aagctgctat
    65941 gagtgctgca cattataaac ttgataattt aattgctttt ttagataata atggacttca
    66001 aatagatgga gatatagaaa gcgtaatgag tttaggttca atagttgata aatttaaagc
    66061 ttttggttgg aatgtaattg agatagatgg acatgatttt gaccaaatat ttgcggctct
    66121 tgatatagct aaatcaacag ttaaaaaacc tacaatgata gttgcaaaaa ctataaaagg
    66181 caaaggaata tcttttatgg aaaatcaagc aggatggcat gggacagctc caagtgatga
    66241 ggagctagaa aaagccttgc ttgaattagg aggtgctgac aatgagtaaa atggcgacga
    66301 gagaagcata tggtaaagca ttagtaaaat taggtcaaat aaatgacaat gttgtagtat
    66361 tagatgcaga tttatcaaag tctacaaaaa cacatgattt ttacaagtca tttccagata
    66421 gattttttaa tatgggtata gctgaacaaa atttaatagg cgctgcttgt ggattatcaa
    66481 ctgctggtaa aatacctttt gcaagtactt ttgctatgtt tgctacggga agggcttttg
    66541 agattataag aaattcagtt tgttatccaa agctaaatgt aaaaatatgt gctactcatg
    66601 caggtcttac tgttggagag gatggagcat ctcatgaaag tgtggaagat attgctataa
    66661 tgagagctat accaaatatg acagttcttg ttccagctga tggagttgaa acagaaaaaa
    66721 ttatttttga aatagctaag tataatggac ctgtttatgt tagattaggg agaagttcag
    66781 taccagtttt atttgatgaa gattataaat ttgaaatagg aaaaggtaca gtcttaagag
    66841 aaggaaagga tgtatcaatt atagcttgtg gaattatggt aaatgaagct ctattagcac
    66901 aagaaaagtt gcaagaagaa ggtataaatg ctagagttat aaacatgtcc tcgataaaac
    66961 ctatagacaa agatttaata ttagaatcag caaaagaaac gaatgctatt gtaactgtag
    67021 aagaacatag cataattggg ggattaggtt ctgctgtaag tgaggtagta ggagagtctt
    67081 gtccaaccat tgtaaagaaa gttggaatta aagatacatt tggagaatct ggtactccaa
    67141 atgaattgtt aaaaaaatat gaattaactt gtgatgacat aataaagact gttaaagaag
    67201 ctattattgc aaaaagaatg taagagatat aaattattca tgtttgttaa taatataata
    67261 tatttaaaag agaggtataa agtgttatat taatttatat ctctcttttt tttatatgat
    67321 atcaatatat caaaaatgta ataaaatatt caaattttta caaaaataga gaaaatattc
    67381 aaatatatat tgactattca gaaaaatatg actaatatat aatctataga gaaactaatt
    67441 ttatatataa tgactactta gataaaagaa tctaaaaaaa ttatgagagg aggagtaggg
    67501 tataaaatca gattataagt tttaagttat tatactaaaa agctataaaa cagttatcat
    67561 gtattttatg ttaaaggaaa ttgtaggtag tttatcgaat tatttaatca gaagaaaaaa
    67621 tttcacatgg ggaggtaact gagatatgtc agaaaaagta aatgttaaaa cagtcatcag
    67681 tttcgcaggt gcgtatgtag caacagtaat cggttctgga tttgcaacag gacaggaaat
    67741 aatgcaattc ttctcttttt atggttttgc aggtattatt ggtgggataa tttcaatggt
    67801 attattctca tggatgggtg cttcagttat gtcaaaaggt aaagagcttc aacttaaaga
    67861 acctatcaaa atttatcgtg tttattgtgg taagtattta gggatattct ttgaatggtt
    67921 tggaccttta ttcttatttg gtgttttcgt agtaatgatt tcaggagcag gagcaacttt
    67981 aactgaatac tatggattaa atccatttgt aggtagagta ggtatggcag tagtagcatt
    68041 attaactgtt tcactaggtc ttgataaatt atcaaagata ctaggtggaa tagggcctat
    68101 aataataatc tttacacttt tagttggtgg tataagttta gctaaaaaca taggtaatat
    68161 aggagaagct gcacaagttc ttagcactgt aaagatagct aaaccagttc caaatgccta
    68221 tctatctggt gtaatatata ctacttataa cacaattgtt gttatggcat tcttaactgg
    68281 attaggagca agtgctgcta acaaaaaaga agctatttgt ggtggtatat taggtggagt
    68341 agcacttatg gctgcagcta taatgatgca cttagctatt ctttctgata taggaaactt
    68401 atattcaaaa gcaataccat cattattctt agcagataag atttcaccag taattggagt
    68461 tttattctca ataattctta tattaggtat atatacaaca gctgtacctt tattatggtc
    68521 agtaactaat agatttgtag atgataaaca tcctaagttt aaattaataa ctttaatagc
    68581 agctatatta ggtcttatag gtggcttcct accatttgat aagctggttg gtattttata
    68641 tccatatact ggatatatgg gcgtcataat actggtttgt gttctttata gacagattac
    68701 aaaaacatct ggatatcaag aaggaatcag tgacaaataa gtatttttta atattgtaaa
    68761 ctcaagagct atatgaaaac aatgaaaatg ttttcatata gctcttttta ttttttagct
    68821 cgtttattat gaaataggaa aaacatgttt tttagttaca ttacatatat ttataattat
    68881 ggataaatat atactaatca ataatttaga gtctaagata agcttataaa tcatatattt
    68941 agaatatatg ggaggagtga taagattgaa tgtaaaattt aatattaaag gtataattta
    69001 tggagttata gctcttgtat tcgttatagg gggatttata ttcataccaa gtatgtttgt
    69061 agagaaatct aaacccatag attatacagt attacaaaga aattcaatac cagaaaagat
    69121 attagatgtg atggataagt atacaaatga agaaagagca ttagcggcta aaatcgataa
    69181 caaaatatac attatagtga ctagaggaaa taataaatat ggtatagaaa tggacaaaat
    69241 agaaagtgtg gttgaggaag gaaaggatgt tttgagagtc aagataaagt ataaagataa
    69301 ggaagattct tttccataca tagttgttga aacaaatatg agtgaacttc cagatagaat
    69361 agaattaaat gcgacgaaat aagaaggtat tttataatta aataactgca tattgtacaa
    69421 taaagtcatg gtttttatta atgtaagtaa attgtatatg gttcttattg ttgtaaaagc
    69481 catgacttaa aatgcttata aagtaagata ttaacagtag tatttattac tatattaaat
    69541 ggggtctgga aatacttaat gttctaatta tttggagtga taattctagt ttcagtaact
    69601 atgtagtcta attgtgcatc atgagtttct tttggaatag aatcgaatat ctggaagtca
    69661 aatgctatgc caatagttat agcatctttt cttaagtttt ctaaaaatct atcataaaat
    69721 cctccaccat atcctaatct atagcaatgt atatcatatg ctacagctgg tacaattaca
    69781 acatctaagg ccttaatatc aattgtaggt gatttttcgt taggttctct tattccatat
    69841 gctccaattc tcaatccctc ttttaaatct gttatttgag tagggattaa tgtatgattt
    69901 tcttttattg tgatgggact tgatactatt ttctttagag acaaaagttt tttgattaaa
    69961 ttatctgttt gaacttcatt gttaaaattt agataaagca ttatattagt tgcatttctt
    70021 atatcttcca tttgaagtaa tttttcagtt attaaatttg aattactaag tacaaagttt
    70081 gcactcttat tttttcttga ttcaataact ttttttctaa attctttttt cataaaataa
    70141 ttcctccctt aatatgttat aatgtgtaca gcaattgtat ttaatataaa ttttagtata
    70201 atttattgta ataaagcaat atatttgggt aaatgtaata aaaataacag ctagtcatat
    70261 aataataata gtttatgtaa aaggagatgt atcttgtgat ttcaaagaag aaagctattt
    70321 ttttaggggt gattttagta attataacag ctatggttac atcagcattt cagttgacat
    70381 tggggaataa agttgtaata tcgaaagaac tctatgaaga ctataaaaaa tatgataagt
    70441 tgttaggact agaatctatt ataaaacaag atttttataa aaaagtatca gatactgatt
    70501 tggtaaatgg agcagcaaaa ggtctatttc taggaacaaa tgataagtat tctggttatt
    70561 atactaaaga tgaaatggag aatctaatta atgatagtga aggttcttat gttggtgtag
    70621 gtatgtatat aggggcatca aaagatggtg gcttagtagt agttcccatg aaagattctc
    70681 cagcagagaa agcaggtgta aaatcagggg ataaattagt aaaagtcaat ggaaaatctg
    70741 tctcatataa aaattcagat gaagctgttc gtatgatgaa gggtaaaaaa gggaaaacag
    70801 ttgaactgac tatattaaga gaagataaac aattgaactt taaagtaaaa acagaccaga
    70861 taattgagaa gagtatagaa agtaaagtaa ttgataatga tttaggatat atagaaatta
    70921 cccaatttat atcaagtaca tatactgact ttgataaagc tttaaaagaa ttaaaagcaa
    70981 aaaatatcaa gggattagta atagacctta gaaataatcc tggtggaatg ctagatatat
    71041 gtaaagaagt tgctgatgag ttaattggtg aaggaactat agtatataca aaagataata
    71101 agggaaatac tgagtacttg aaatctgata aagaaaaatt gggacttcca atagtagtgt
    71161 taactaatgg agaaagtgct tctgctgctg aaatattaac agctgcaatc gttgataata
    71221 aagaaggtat ttctgtaggg actacaactt ttggtaaagg gttggtacaa tcagttgtaa
    71281 gattgaagga tggaacagga tacaagttaa ctactgctca atattttaca cctaatggag
    71341 attatatcaa tgaaaaaggc ataaagccta ctattgaaga aaaagatgaa aataaacagt
    71401 tagatgtagc gactaaatgg cttagagaac agattgataa ataaagttta atttttataa
    71461 acttatattg taaaataaag aaccttaata aaaggagtgt ctcaaaatta actttttagt
    71521 ttatgaggca ctcttttgtg ggtctttaaa ataaagtgtg tattcttcta aaaagtagtg
    71581 ataattaaaa tattaaatat cactatatat aaggaaggtg cacacttttt atgtttaaca
    71641 aaaacgaaat attaaaaaaa cttattgaag aatatgatgt taaaacaact acagatattc
    71701 aagatatgct aaaagattta tttgcaagta caattcatca aatgttagaa gcggagcttg
    71761 acgaccattt aggttatgat agatatgaca ataagaataa aaatactaaa aactctagaa
    71821 atagatatag aaataaaaat gttaaatcag attttggaga aataacttta aatattccaa
    71881 gagatcgaaa tggagatttt gaaccaaagg taattagaaa ttatgaaaat gatatatcag
    71941 gaatagagaa ccaagtgatt ggaatgtact caaaaggtat gtctactcga gatatatcaa
    72001 gccaccttaa gagtatttac ggaattgata tatctcattc tctaatagct aaaattactg
    72061 atagagttct acctttagct catgaatggc aaagcagagc tcttgatatg gtttatccaa
    72121 ttgtatttat gggtgctata cattataaag ttagaagtga aggtagaata ataaacaaag
    72181 ctgcatatat agccataggt gttaacttag aaggtataa
//
LOCUS       DACTGW010000002        54919 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.002, whole genome shotgun sequence.
ACCESSION   DACTGW010000002 DACTGW010000000
VERSION     DACTGW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54919
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.002"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            123..1358
                     /locus_tag="I9573_000075"
     CDS             123..1358
                     /locus_tag="I9573_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAT4794722.1"
                     /translation="MGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITSGVTGMF
                     CHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLRGSLASLLEVESGSNDPIAY
                     MLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFYTIF
                     ITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIMLFF
                     MLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVGLRG
                     AASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTPVLK
                     TFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGSTII
                     KTGDILVLSGDRIEELIQL"
     gene            1434..2246
                     /locus_tag="I9573_000076"
     CDS             1434..2246
                     /locus_tag="I9573_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT4794723.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRIL"
     gene            complement(2586..3767)
                     /locus_tag="I9573_000077"
     CDS             complement(2586..3767)
                     /locus_tag="I9573_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAT4794724.1"
                     /translation="MKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYSWKLVP
                     TDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRNTEL
                     GRQYLVNALENDPLGMRAIDTVKQSDAKEWAIRMNGKGYSYKTIDNYKRSLKASFYMA
                     IQDDCIRKNPFNSKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVILLL
                     ETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISKAKTKNGKRELPLTERAY
                     QALKRILKNRGKAQPLVVGGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHKDKL
                     PNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELERLA
                     S"
     gene            complement(3748..3882)
                     /locus_tag="I9573_000078"
     CDS             complement(3748..3882)
                     /locus_tag="I9573_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAT4794725.1"
                     /translation="MYYFLEMSDTEIADIMNYTREAVFKHRHRALETMKEILNEKTRQ
                     "
     gene            complement(3882..4172)
                     /locus_tag="I9573_000079"
     CDS             complement(3882..4172)
                     /locus_tag="I9573_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794726.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELRRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKNVTMY"
     gene            complement(4438..4668)
                     /locus_tag="I9573_000080"
     CDS             complement(4438..4668)
                     /locus_tag="I9573_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794727.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            complement(4692..4883)
                     /locus_tag="I9573_000081"
     CDS             complement(4692..4883)
                     /locus_tag="I9573_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HAT4794728.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            complement(5191..6102)
                     /locus_tag="I9573_000082"
     CDS             complement(5191..6102)
                     /locus_tag="I9573_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAT4794729.1"
                     /translation="MEKGRLLQNENINKAIQVSKITLIINLGLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDKEHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVSSGIALTAAVLSIVIKEWMYWYTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASIVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELPYSP"
     gene            complement(6114..6812)
                     /locus_tag="I9573_000083"
     CDS             complement(6114..6812)
                     /locus_tag="I9573_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4794730.1"
                     /translation="MKRILSSTIQVFKQIKSDPMMFAACFTPFVMGALIKFGIPFLDG
                     ITDFSLQTYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLMVALVW
                     YYFLIKRLFRKLAG"
     gene            complement(6809..7492)
                     /locus_tag="I9573_000084"
     CDS             complement(6809..7492)
                     /locus_tag="I9573_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4794731.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALTGHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTIALIAISTI
                     IAVILGFVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPDIFRFFPFTACMNLITGKSILLSFDMVLVIATLIILYIVARHTVKHM
                     WRSLGGVKL"
     gene            complement(7489..8334)
                     /locus_tag="I9573_000085"
     CDS             complement(7489..8334)
                     /locus_tag="I9573_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4794732.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KILTGLNTSYKGSVKVAGTEIREHTNRFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     DKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNNRLMKDIILAEKKRGKTIIITTHNMYDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIHYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            complement(8438..9025)
                     /locus_tag="I9573_000086"
     CDS             complement(8438..9025)
                     /locus_tag="I9573_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT4794733.1"
                     /translation="MSLPRFTEQEKEIINSKLLIEGEKLFSLYGLKKVTVDDLAAAVN
                     ISKGSFYAFYPSKEHLYVEINFRLQKELFTSIETTVKKKKYKNHRDLAKDVIMLSLTG
                     VITSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEGLGVVFLVPHTVIIKS
                     LYSVLSCLEQFKEDEELNMVQNLLVNGIIQQVVAE"
     gene            complement(9097..9954)
                     /locus_tag="I9573_000087"
     CDS             complement(9097..9954)
                     /locus_tag="I9573_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT4794734.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERNSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQLGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFRFDYTNKVFDLIHVPKKELLTLELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            complement(10167..11060)
                     /locus_tag="I9573_000088"
     CDS             complement(10167..11060)
                     /locus_tag="I9573_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT4794735.1"
                     /translation="MFKKKEKPVKETKVRTMKVGTHKKSVIALWVVLIASVSFGVYKN
                     FTAIDMHTVHETETIQLRLNDTNGVENFVKNFCKSYYTWDNSKEAIEARTQAISNYLT
                     KELQDLNLDTIRTDIPTSSTVTNVLIWDIKQSGTNDFIATYEVDQQIKEGEQTSNVKA
                     TYTVKVHVDKDGDMVIVQNPTIAPAIEKSDYEPKAQEADASVDADTVNDATAFLETFF
                     KLYPTATEKELAYYVAGNVLEPIGRDYLYSELVNPMFTDDRDNVKVRVAVKFIDNQTK
                     AMQVSQFELVLHKDSNWKIIM"
     gene            complement(11076..12083)
                     /locus_tag="I9573_000089"
     CDS             complement(11076..12083)
                     /locus_tag="I9573_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAT4794736.1"
                     /translation="MKLRHIAIVGSLFSILFSLVLFFGVLISAEDDDGGNGYSSNYVG
                     MNLSAEVLKHQPMVEKYAKENGISEYVNVLLVIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGISDINVVIQSYNYGGGYVGYVAGNGKKHMFNLA
                     ENFARDKSGGQKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLVVNQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYNKAGVSLPRTAQNQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNTQMYHAGDPIGYADLNNAYWQQYLICAGRIK
                     Q"
     gene            complement(12080..14215)
                     /locus_tag="I9573_000090"
     CDS             complement(12080..14215)
                     /locus_tag="I9573_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAT4794737.1"
                     /translation="MKERIKSVFTRKKIFRFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYADNNWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     RLYLSNATGYVVQEAYKLDFINDMADSIGRSIQTLAGVTQNGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKVNDFSSDISTAS
                     LDLGTKIMLPDSDSKGKDSVDLIRDSLFSIQVQQPWLLLQFGNSGIEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTILQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVYFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRLARAVSAGGVAGAVAGGVVASSKSTRQNGTKNNRGNTSSSMGQK
                     AGSAVGAVLDTKNKVKDKATAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGVVQE
                     KENRQSGRLKKQQLHRQNIADKRMELQKAQESRQAGKKTDGSATSGATRPHERPATTS
                     KVNVEKQQEVKRPAIASTVKNDEPIKKNVVKERPLSSGDTSAKTVNQETTRQNYKTDR
                     VTKTTVQKHTQQNTEKNKRTVFKKGQKKK"
     gene            complement(14215..16647)
                     /locus_tag="I9573_000091"
     CDS             complement(14215..16647)
                     /locus_tag="I9573_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT4794738.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSSRSIQEQSKKLVTGRLKDVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTDQSMNLKSVKQSAFLTFKEFIHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLDKNDFGYLIEHIYGRDGVAYEDYEYSLPKKKLKKE
                     TLITHYDLIRPTRCLVEESQRHLRLEHEDSESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQSGSETSSNVVDALDSVD
                     ELETDLDQSRESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYIGYSVDTGRNVYLQPS
                     LASQGVKDTVTNALASAFVESLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDRENAGLLDPFVIMQNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRAVTQSDQRGLLHVIEELRKEDTPISCNIADHIDSFTDYDFAHLLFSDGTV
                     QNAISLDNQLNIIQVADLVLPDKDTTFELLSVSMLIVISTFALDFIHSDRSVFKIVDL
                     DEAWAFLNVAQGETLSNKLVRAGRAMNAGVYFVTQSSGDVSKESLKNNIGLKFAFRST
                     DINEIKQTLEFFSIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFEELLHAF
                     DTRPPIQRNEVE"
     gene            complement(16634..17023)
                     /locus_tag="I9573_000092"
     CDS             complement(16634..17023)
                     /locus_tag="I9573_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT4794739.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDLPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITFALRPKITY
                     AGKAVKLDKQVLHEAITAVRSVSYVPD"
     gene            complement(17136..17639)
                     /locus_tag="I9573_000093"
     CDS             complement(17136..17639)
                     /locus_tag="I9573_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAT4794740.1"
                     /translation="MVEDMRVYIANLGKYNEGYLVGDWFSFPIDEEDVAERIGLNERY
                     EEYAVHDTENFPIEIGEYISIEELNEMYEMICELPDYITDSLDEFVSNYGSLEELYDH
                     RDDMILYAGCEDMTDVAYYFIDELQALGDIPPSLQNYIDYEAYGRDLSFEGTFIETRY
                     GMCEIPW"
     gene            complement(17655..18152)
                     /locus_tag="I9573_000094"
     CDS             complement(17655..18152)
                     /locus_tag="I9573_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAT4794741.1"
                     /translation="MNEMRVCIESRLHTSEEIRSAWFTLPIDEEELEEQIGVPIDSED
                     YHIVEKELPFADEVGEDATIEKLNDLYHTFESLPADLQEDYGELMCYFTSLDELHQHR
                     HDVIHYSWCKNMADVARHLLDNDPAFSAIEERLTRYFDYEAYGQYLDDNGEFIETDHG
                     IYELP"
     gene            complement(18173..18361)
                     /locus_tag="I9573_000095"
     CDS             complement(18173..18361)
                     /locus_tag="I9573_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794742.1"
                     /translation="MKDIFSDMQASIGCTYISDLPHHKRMVWQEMKRLNLADYPQKQL
                     EDFAGYVFGVPYTSIAEA"
     gene            complement(18438..19076)
                     /locus_tag="I9573_000096"
     CDS             complement(18438..19076)
                     /locus_tag="I9573_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HAT4794743.1"
                     /translation="MEYKYTKIDLNKWNRGKLFQSYIDNMRIVMSLTVDIDVTKFIEF
                     SKKHDLKFYPAMIWAVSKVVNEHDEFKYSWDNNGNLIKWDYISPSYTEFHKEDENFTK
                     IVTDFSDSIFEFHKRFILDREKYKTERAFVENQSLNFFDVSCLPWVKYNHFDVHVFDE
                     GKFLAPVITWGKYELEQERYIMPLTMNIHHAVADGFHLSRFFNEVQELINSI"
     gene            complement(19396..19617)
                     /locus_tag="I9573_000097"
     CDS             complement(19396..19617)
                     /locus_tag="I9573_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794744.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALVAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(19618..19752)
                     /locus_tag="I9573_000098"
     CDS             complement(19618..19752)
                     /locus_tag="I9573_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAT4794745.1"
                     /translation="MWELIKDLLLVSLGMGIGVVLMCIMSVGKEADRHMIELKESEEK
                     "
     gene            complement(19759..20880)
                     /locus_tag="I9573_000099"
     CDS             complement(19759..20880)
                     /locus_tag="I9573_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAT4794746.1"
                     /translation="MAGISREHLSRIEAGKVALTEDMKHKLLEAVEKFNPDAPMFLLF
                     DYVRIRFPTLDIQHVIKDILKLNIDYMLHEDFGHYKYTEHYYIGDVFVYTSQDKEKGV
                     LLELKGKGCRQFESYLLAQERSWYDFLMDALVDGGVMKRIDLAINDRTGLLDIPELIE
                     KCKNEECISKFRSFKNYGSGELVKHNEANKDGMGHTLYIGSFTSEVYFCVYEKNYEQS
                     SKLGIPIEEVPIKNRFEIRLKNEHAYYAIRELLTHYDAELTAFSIINQYIRFADKEAN
                     KRKSDWKTNARWSWFIGEGRPPVKLTTKPEPYTLQRTLNWLQRQVAPTLKMLKQIDAG
                     NQMDYLKTIEQQAKLTDKHKQIIRQQTASVDELIEKEKE"
     gene            complement(21160..22554)
                     /locus_tag="I9573_000100"
     CDS             complement(21160..22554)
                     /locus_tag="I9573_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT4794747.1"
                     /translation="MRMILNKGHRIRARDKNLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLDNGLTLSPYNFITILIATGICALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMENGLLHIHCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRISIDEVKAENGCLRLMKNLV
                     WEYDVLPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEDMIDCVNAFYEGMVQRSEEMKQHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAFVRQ
                     DGTATCEAKGDGTD"
     gene            complement(22664..23170)
                     /locus_tag="I9573_000101"
     CDS             complement(22664..23170)
                     /locus_tag="I9573_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HAT4794748.1"
                     /translation="METVELRFQYTQSEYIKAERQYLISSKTIHKYDIALVAVFLLLS
                     VVYMLFSSFSVFSILIFGLIIVVTALGSYLYILMPILKFKQTAKYHEEYTLVFSKENI
                     KFKTQSIDSEVKWDIYSALWESHDFYYLIQAPRLYTLIPKRIFKDLNEKQLFEEIAQS
                     RVKTTKHI"
     gene            complement(23227..23769)
                     /locus_tag="I9573_000102"
     CDS             complement(23227..23769)
                     /locus_tag="I9573_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HAT4794749.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKRRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            complement(23794..24252)
                     /locus_tag="I9573_000103"
     CDS             complement(23794..24252)
                     /locus_tag="I9573_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAT4794750.1"
                     /translation="MKLKNPMLVVTDIDKSVEFYKKVFGLYVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGNNFEVYFEEEDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            complement(24359..24772)
                     /locus_tag="I9573_000104"
     CDS             complement(24359..24772)
                     /locus_tag="I9573_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HAT4794751.1"
                     /translation="MLDKIPNAEEMTALVGQSLYSVWNELCALIDEKYEMECLWNKGG
                     KAWNYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTYRDNYSKEVQRIYDET
                     KTYHDGKWLMFEPTDTTLFDDFVKLLRIKRKPNRK"
     gene            complement(24868..25251)
                     /locus_tag="I9573_000105"
     CDS             complement(24868..25251)
                     /locus_tag="I9573_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAT4794752.1"
                     /translation="MRLTNGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGTTVDWYIKAD
                     DIVLKNKGGQAGNPQNNAPQGQQKK"
     gene            complement(25266..25589)
                     /locus_tag="I9573_000106"
     CDS             complement(25266..25589)
                     /locus_tag="I9573_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAT4794753.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQRINGRYTLVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            complement(25613..25801)
                     /locus_tag="I9573_000107"
                     /pseudo
     CDS             complement(25613..25801)
                     /locus_tag="I9573_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843347.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25814..28474)
                     /locus_tag="I9573_000108"
     CDS             complement(25814..28474)
                     /locus_tag="I9573_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAT4794754.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVSI
                     VEQVMNDGSISSTFNEGHLTVEGEDAYCIDINTAFKNGYKTKADASTRMSADQIADVA
                     LSIEYVKQYTDIHSGISSKHAYLLRQLVVWQRLSVQPDWQWDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNTKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLTTLTTDTSGNTEAVEVRATIVYIKELSAPAGFKIDKT
                     VYSLSVEVGKTATLKVSDTPKVTDTLIELFKIDMETQKSNPQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLADKYKVSWDSFYTQDGKNVLPL
                     GTLTVEETKSPSGYLLEGTYMQADGSEEQIKGMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETKDTKAQGGATLKDTAFEIISLNENAVLVEGKLYKKNEVLKTIH
                     TDIEGIASTSADLLPYGKYRLSEQKPPEGYLTDGAKPIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALLYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGKDVTITDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIINDKRIENDYIFTADSETMEVKIEFTFDASNLGGKQLVTFEELYD
                     LSNPDEPIKVAEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDTPTKTDSPKTGDNT
                     NILAFAIMMFVSAGGLAGTYFFKHRKMKKS"
     gene            29023..29640
                     /locus_tag="I9573_000109"
     CDS             29023..29640
                     /locus_tag="I9573_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAT4794755.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFIYIYMGKEFGAKRNNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDREQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(29797..30249)
                     /locus_tag="I9573_000110"
     CDS             complement(29797..30249)
                     /locus_tag="I9573_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794756.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            30697..32055
                     /locus_tag="I9573_000111"
     CDS             30697..32055
                     /locus_tag="I9573_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT4794757.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTIMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            32328..32708
                     /locus_tag="I9573_000112"
     CDS             32328..32708
                     /locus_tag="I9573_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAT4794758.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            32833..33033
                     /locus_tag="I9573_000113"
     CDS             32833..33033
                     /locus_tag="I9573_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794759.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            33229..33957
                     /locus_tag="I9573_000114"
     CDS             33229..33957
                     /locus_tag="I9573_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT4794760.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            33979..34896
                     /locus_tag="I9573_000115"
     CDS             33979..34896
                     /locus_tag="I9573_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HAT4794761.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            35038..36222
                     /locus_tag="I9573_000116"
     CDS             35038..36222
                     /locus_tag="I9573_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT4794762.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            36329..36517
                     /locus_tag="I9573_000117"
     CDS             36329..36517
                     /locus_tag="I9573_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAT4794763.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            36667..37317
                     /locus_tag="I9573_000118"
     CDS             36667..37317
                     /locus_tag="I9573_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAT4794764.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            37420..38358
                     /locus_tag="I9573_000119"
     CDS             37420..38358
                     /locus_tag="I9573_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAT4794765.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            38384..39235
                     /locus_tag="I9573_000120"
     CDS             38384..39235
                     /locus_tag="I9573_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAT4794766.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(39520..40254)
                     /locus_tag="I9573_000121"
     CDS             complement(39520..40254)
                     /locus_tag="I9573_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HAT4794767.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            40380..41096
                     /locus_tag="I9573_000122"
     CDS             40380..41096
                     /locus_tag="I9573_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAT4794768.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            41230..41922
                     /locus_tag="I9573_000123"
     CDS             41230..41922
                     /locus_tag="I9573_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010296823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4794769.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            41912..43093
                     /locus_tag="I9573_000124"
     CDS             41912..43093
                     /locus_tag="I9573_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAT4794770.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKMEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            43375..43830
                     /locus_tag="I9573_000125"
     CDS             43375..43830
                     /locus_tag="I9573_000125"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012423319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAT4794771.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            43820..44545
                     /locus_tag="I9573_000126"
     CDS             43820..44545
                     /locus_tag="I9573_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HAT4794772.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLNQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            44517..46898
                     /locus_tag="I9573_000127"
     CDS             44517..46898
                     /locus_tag="I9573_000127"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAT4794773.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDNAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSCGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(47103..48332)
                     /locus_tag="I9573_000128"
     CDS             complement(47103..48332)
                     /locus_tag="I9573_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAT4794774.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            48932..49846
                     /locus_tag="I9573_000129"
     CDS             48932..49846
                     /locus_tag="I9573_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794775.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGVKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            49870..50505
                     /locus_tag="I9573_000130"
     CDS             49870..50505
                     /locus_tag="I9573_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HAT4794776.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTAQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            50881..51597
                     /gene="pyrF"
                     /locus_tag="I9573_000131"
     CDS             50881..51597
                     /gene="pyrF"
                     /locus_tag="I9573_000131"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAT4794777.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGADFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            51623..52666
                     /gene="carA"
                     /locus_tag="I9573_000132"
     CDS             51623..52666
                     /gene="carA"
                     /locus_tag="I9573_000132"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAT4794778.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGEILTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIAPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            52719..>54919
                     /gene="carB"
                     /locus_tag="I9573_000133"
     CDS             52719..>54919
                     /gene="carB"
                     /locus_tag="I9573_000133"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HAT4794779.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYL"
BASE COUNT        18775 a         8765 c         9817 g        17562 t
ORIGIN      
        1 ggggatagcc tgtggagaat tagtaagaca tattttagta tgcaaaattc tatgaagcag
       61 gaactctgtg actttagtca tgggaggttc agaactatga attaacaaaa caattatgtt
      121 ctattggact aatatttata atgttttttg gagggtttgg aacaaactgg aatatggcta
      181 aacctgttgc tataccttct atattaatgt cttcactggg tgtaataatt acgtctgggg
      241 ttacaggtat gttttgccac atggtaatag gaactagttt acttgaaggt ttattaattg
      301 gggcaattgc agcttcaaca gatgctgcat ctgtgtttgc tgtgttgcgt tcacaaaaac
      361 taaacttaag gggttcactt gcatcattat tagaagttga aagtggaagt aatgacccaa
      421 tagcgtatat gttgacacta attcttttga cattgatgaa taattctggg gtagaactta
      481 ttgcaccaat ggtaataaaa caaatagctt ttgggttagc tataggattt atactagcaa
      541 aattatctgt ttatatttta aggcattcaa acttcgagat agaaggattt tatactatat
      601 ttataacagc tattgcaata ctttcttatg catttagcga gtatcttgga ggaaatggat
      661 atttaagtgt ttacatttca ggtattatta taggaaatag taggatacct cttaaaaaaa
      721 gtttggttca tttctttgat ggaatttcat ggattatgca aataatgtta ttctttatgt
      781 taggattgtt atctttccct tctaagttac caaatgtatt tggtatagca atggccatat
      841 ctatattcat gatagttatt gcaagaccat tagcaacatt tatagttcta gcaaaattta
      901 aattctcaaa taaagaaaaa ttattcatat cgtgggtagg tcttagaggt gcggcttcag
      961 tagtttttgc aatttatgct gttactcaag gagtagccat agagaatgac atattccata
     1021 taatattttt tatagcgttg ctttcagtag gaatacaagg ttcattgata ccaactgtag
     1081 ccagaaagtt ggatttagtt gatgataata cacctgtatt aaaaacattt aatgattatg
     1141 atggggaaat tagctctaaa cttatcgaag taaatgttga tgagggtaat aaatgggcta
     1201 ataagagtat aatagattct aatataccag acgatatatt aatagttatg ataaaaagaa
     1261 aggaacaggt tttaatacct aaaggttcaa ccattataaa aacaggggat attttggtat
     1321 tgagtggaga tagaattgaa gaattaatac aattataatt tattttgtat tgactttaac
     1381 ctaaggggaa agtttaaact taaattaatc aaatctaatg cgcaggtgat gagatgtttt
     1441 ctataggaat gttttctaaa ataaataaaa taactacaaa gacattaagg tattatgaag
     1501 atatagggtt gttaaaacca gaatatgtag atgaatttac aaaatatagg tattatacaa
     1561 cagagcaatt acctaaatta catcagataa taactttaaa acaaatgggt ttatcattag
     1621 ctgagataaa aaaagctgta gaaaatcctg aagaagttga aaatatttta atgaataaag
     1681 aaaaagaaat gttggatgta attaaaagtg aagaatataa aataattaaa ctacgtaatt
     1741 accttatgaa tttgaaagga gaagtcaata tgaatgatgt aattattaag tctttaccaa
     1801 aagttaaagt tgcttctatg agaaaagtgt taaagggata tgatgaactt tttaatctat
     1861 ttccaaatgt aatggctgaa gaaatgatga aaacagggtg tacatgtgca gttcctgatt
     1921 attgttttaa tatatatcat gatggtgaat atagagagac tgatatggat gttgaaatgt
     1981 gtcaagctat aaatgattta aaggaagata cggatatatt aaaatttaaa gaattagatg
     2041 cagttgaaaa tgcagtttgt atattacaca atggtccata ttcaacatta ggaaaaactt
     2101 atgcagatgc atttaaatgg atagaggaca ataaatataa agtggttgga aatcctagag
     2161 aatcttatat agatggtata tggaataaag agagtgaaga agactggttg acagaagttc
     2221 agataccagt gaaacgcata ttataatatg tctacaaagt ataaataaga ataaaaaagg
     2281 tagtgtctca aagtaaactt tttagtttat gagacactct ttttcatatg aaatcaagtg
     2341 tatttttata attccgatta tgaaaaaagg cttatctcta tttttggaca gcctcttatt
     2401 tttttattat aaaaacttac taaatttctg attatctttt cataagttgg tagatgccga
     2461 tatatcccgc attttcgggc tgtatgggca ttttcttttt ggaagatacc ttgcataagc
     2521 tggcatttac cagcatgaaa atgcaaccaa aagtagtaaa taagtagtaa gatacatttt
     2581 cgatattaag aagccagcct ttctaactct gcctttgcag actggaaagt accgtgagca
     2641 taatacccta atgtcatggt tatgtttgcg tgtcccatga tgtattgcag ggtgtttggg
     2701 ttcattccct tatttgccat gttcgtacag taagtatgtc tgaatgaatg gggcgtgatg
     2761 tttggtaact tgtccttatg ggtcttgttg tacttcttaa tcaacccacg caccatactt
     2821 tcataatttc ctgctacctt tggcaatccc tcacggttca agaataagaa attgctataa
     2881 ccaccaacaa caagcggttg tgcttttcct cggttcttta aaattcgctt gagtgcttga
     2941 tatgctcttt ctgttaatgg aagctctcgc ttaccgtttt ttgtcttagc tttggaaata
     3001 tagtagccca cctctgtatc acgcaatagc tggtggtcta tgttaatcac acggttaagc
     3061 atatcaatgt gtgttgtcag tccgcataat tcagagatac gaagtcccgt ttccagcaga
     3121 agaataactt catcacgata cttgctgtaa acattatctg tttccataaa ggcaaggagt
     3181 ttttcttcct gctctggtgt caggataacc tttgcttccg tatcatcttc cagaacatca
     3241 ctcaacttgg aattaaaagg attttttctg atacagtcgt cttgtatcgc catatagaat
     3301 gaagccttta gggaacgctt atagttatct atcgtcttgt aagaataacc tttgccattc
     3361 attctgatag cccattcttt agcgtcggac tgcttgaccg tatcaatcgc cctcataccc
     3421 aatgggtcat tttccagtgc gttcacaaga tactgtcgtc ctaattccgt gtttctcttg
     3481 atgttctttc tttgattgtt ctttttttcg taaagctggc agactgtcat tttaccgccc
     3541 accgtgtcga taccgtcgtc aaggtctttt tttatctgct tttctttttc cctcaacgat
     3601 atatcatcac gttttccagc aggtgtcttg tctgtgggta caagcttcca agaatataca
     3661 aactgtggct tcccgaatat atcggtatat ttataaacgt atcttccgtc ttttctctgg
     3721 ctctctccaa gacgcaaatt gcgtccttta ctgtcttgtc ttttcattta gtatctcttt
     3781 catagtttct agtgcacggt gtctgtgttt gaacacagcc tctctggtat aattcataat
     3841 atctgcaatc tctgtatcac tcatttccaa gaagtaatac atcaatacat tgttacgttt
     3901 ttttcgggta gctgtttgag agcttctgca agttcatcat caaggacttt aatatctatg
     3961 ccacacacat taaaaattgt gtagtttgtt tcgtagtcgt cccatgttgc cagactttct
     4021 accgtaatgt ctggtaattc gctaaaagat atttccttgt tttttcttcg tcttaattct
     4081 ttgcggtagt tccagataat acctttgaca acttttttca gcttacattc aaactgacac
     4141 tgaattgttt tttggaagtc agacggtttc ataatctcac ctcctttcca gctatgaatt
     4201 gaaaagtgtt tatccctctt tccgcaccat atctgtacag caaggtgtga tttgcttcca
     4261 aaaaaatgaa aataattaaa aaacttttcg ggtattaaaa aagcccgcac aaaatataaa
     4321 ttttgtacga gctactggta tagcgtattt aattgtaaat agtatgctgt taaattgggc
     4381 ttgaatatac tgaattgtat ttaaagcgtt tcatacggca agcccccttt acaacattta
     4441 aaccttaacc tttgtctttt tggtaatcgt ataaatatat tcctctggtg ttggccgctt
     4501 atttccaaag tatcgtttaa aatttgcctg cacgtctggg tctgtacttt ccatagtttt
     4561 gtctatggta tcttgtagca tagccctaaa ttcagataca gaaacacctt gagctttagc
     4621 accaactttc ataatataat tcatatctcg tcttttaagt cctatcatta gaaccaactc
     4681 cctaaaataa gttaatcttc tttttcaaaa attaaatcta tctttcgcat acagcctttt
     4741 gcgtcaattt ctgtgggtac aaaccctgcg tagtgatacc ccgtttttgc ataactgtca
     4801 ataatttctt tgtgtttatc agtacaaagg aatacaactc ctttcgcatt ataattaatt
     4861 tctacatact catatttttt cattcttcaa aactcctttc aaatacaaat ttatctttgg
     4921 atttattgac ttttacaaac tgggatttat cgttcaaatt tcataggtat atatctcata
     4981 ccatcggata attgctctta atacatcttg tcatcctcgc tattttttga acgattgtta
     5041 gaatttctcc tttggttata cttcttcata attacaattt taagtaagcc aacgacaatc
     5101 aaaaccataa caatgataat ccaccagtat tgttccataa cctacttccc ctcctttccc
     5161 tttcaatagt cttaaatcat cttccctgtc ctatggagaa tagggaagtt cattcgtatt
     5221 aacatgtacc atacaatgtt tacattgtgg aaagttgttt tcaatcgttt gatggactcc
     5281 ctcagcaatt ctatgagcgt cataaagagc taaattacca tcagctaata tttcaatatc
     5341 tacatacatt ttcgctccaa acatgcgggt ttttaattca tcaataccat caaccccttg
     5401 ctgctgaagc acaacttccc taatcttcgt ttcagtagca ttgtcacaac tatggtctac
     5461 cattttgtta atgctttctt tgaaaatgtc caaagcagct tttaagatac aaccgcaaat
     5521 cacaatgctt gcaatagggt cgagaatagc atagcctaaa cgtgctccta aaatgccaat
     5581 taaggaacca accgacgaga gtgcatcaga gcggtgatgc catgcgtcag ccataagtgc
     5641 gtcagaattt gtatgctttg cagcacttct tgtataccag tacatccact cttttataac
     5701 aatggataag actgccgccg taagtgcgat tccactagag acagaaagcg tattgtactg
     5761 tcctgaaaag attttcttga tacctgaata tcctattcca gcacccgtta tagccaatgc
     5821 aacagagaga ataatggaag ccacacattc catacgttca tgcccataag gatgttcttt
     5881 atcaggctgt ttttctgata ttttaattcc aaccatcaca acgattgtgc ttaatacatc
     5941 agaagccgag tgtacagcgt ccgacagcat ggcacttgac tttcctatat atccggcagc
     6001 aaatttaagt agcgataaac ccaggttgat gattagtgtg attttggaaa cctgaatagc
     6061 cttatttata ttttcgtttt gcagaagtct acctttttcc ataatatgcc ttcttatcct
     6121 gccagcttcc taaacagacg ttttataagg aagtagtacc agaccaaagc taccatcaat
     6181 cctatggaga aataaagaac cgcttcattc tgcatggctt tcgctaccca aaatgatgga
     6241 aggaagccaa ccgcgaactg gtagtaacta tcaataaaaa agggagctgg tattccgagt
     6301 aatgtaagtg cagctagttt tgtaactgcc ataccctcta atttattgct tgataatgtt
     6361 ataatcataa gcgaaacaat aattgcctgt actgagccga gaagcgccaa acatattgtc
     6421 attccaatgg acaacttttc taatgaaaaa agcaacagta caataaaagc gatgaccgcc
     6481 gaaataattg agggtactcc caaccttgta aagagatacc ccgacttacc aagaggggta
     6541 atcgaaaagt accgtgacac tttatcgtcg atttcctcta atgtaatcat tgcaaatgca
     6601 aagcagagca atacaggagc catgatagaa agtaataaat caaagatcgg atagtatgtt
     6661 tgcaaagaaa aatctgttat tccatcaaga aagggaatac cgaatttaat caaagctccc
     6721 atgacaaaag gcgtaaaaca agctgcaaac atcatagggt cgctcttgat ttgcttaaat
     6781 acctgtatgg tactggataa tatccttttc atagctttac acctcccaaa cttctccaca
     6841 tatgcttaac tgtatgtcgc gcaactatgt agaggataat caacgtagca attacaagta
     6901 ccatatcaaa cgatagtaaa atgctttttc cagttatgag gttcatgcaa gctgtgaacg
     6961 gaaaaaagcg gaatatatct ggtaacttta caaacagacc aacgataggt ggaacaaagc
     7021 aaacaatttc tatcggcata atcacaatga gaaattggtt caagtttgaa atttttgtag
     7081 ccgcgataat gccaagcata gtaaagattg ctgatgtcaa agctgtacca actgcgatac
     7141 caagtaaatg attgctgccg gaaacaaatc ccaatatcac cgcaatgatt gtagaaatag
     7201 caatcaatgc gatggttttt gaaagtatat actccgatac ttttacaggc gatactacca
     7261 tagcatttaa taccttttgg cttttttcta aaaggacaat cgcccccata aaaaacaatc
     7321 ccatagctgc cgggtcagag tatatcatga tagaagcaat atccgttttc caatgtcccg
     7381 tcaaagcggc aatcccacac acatataaaa ctgttaatat aaagtagata aaatagaaac
     7441 catatttcca ttggaaatgt atatcaccac aaattaaacg tcccaatctc attgaagtgt
     7501 cctccctgta atatcaacaa aaatatcatt taatgtaggc tcactactat gaatagaaag
     7561 cagacggttt tcggaaatta aactttttaa caacttatct tctgtggttc tatcaagcag
     7621 acattctccg gttttctcgc ctttatcata gtaggtatag tgtattttgg cagctcccct
     7681 tgacataatc aaattatgtg gactatccaa tgcactgact tttccggcaa caataaaggc
     7741 tacttggtca catagctctg ttgcgtcata catattgtga gtagtaatga taatcgtttt
     7801 tccgcgtttc ttttccgcaa gaataatatc tttcattaaa cgattatttg tagggtcaag
     7861 accgcttgta ggttcatcaa ggaataatat atcagggtca tgtactaacg ctttaatgaa
     7921 attcaagcga gaacgcatac ctttggaaaa gtcagcaact tttttgtccc catcattttc
     7981 taagcctacc atatgcaata attcatcaat agaccgaggt tgcttgtcat atagagaagc
     8041 aaaataggcg agattttgtc gtgctgtgaa tttctcgtag caggttgaaa attcaaagtc
     8101 tactccaata ttttcataaa accggtttgt atgctccctt atttctgtac ctgcaacttt
     8161 tacgctgcct ttataacttg tattaagccc agttaaaatt ttttgaatag ttgattttcc
     8221 agcaccagag ggtcctaaaa aaccgaaaat ttctcctctc ccaacatgga aacttacatt
     8281 ctcaacaaac ggtttgtcag tataactaaa atacagtttt tttacatcaa tcattattct
     8341 ttcctccttt aactttatga cgtttataga tatatagtca tataaactgt aaaaaatttg
     8401 tttggccact tgcctgtcaa ataaccaaac aaaattatta ctctgctact acttgctgaa
     8461 taataccatt cactaaaaga ttttgtacca tattcaattc ttcgtcctct ttgaattgtt
     8521 ccaaacagga taaaacagaa tacagggatt ttatgataac cgtatgcgga accaaaaata
     8581 caacccctaa accttccagt atctccaata tacgaatatc actgtacata tgattttcaa
     8641 aaatatctga cgatattttt ctttgcagat agtccattaa agataaatca atctgtgaaa
     8701 gaataggcga agtaatcacc cccgttaaac ttagcataat aacgtccttt gctaaatctc
     8761 tatggttctt atactttttc tttttaacag ttgtttctat acttgtaaat aactcttttt
     8821 gtaagcgaaa attgatttcc acatatagat gttctttact gggataaaaa gcatagaatg
     8881 aaccttttga tatgtttaca gcagctgcta aatcatcaac tgttactttt tttaagccgt
     8941 ataatgaaaa cagcttttct ccctctatca aaagtttact gtttatgatt tctttttctt
     9001 gttccgtaaa tcgtggcaaa ctcataaccc ccttatgact aaaatgtttt tatcgtcatg
     9061 aaaagtataa catttactga ttatataatc aatatactat ccaagatatt tatgcaaggt
     9121 agcttccaat gcgtttattg tagtgtccgt gtcctcatta aaaatcatgt gacgattaag
     9181 ctctaaagta agtaattcct ttttcggtac atgaattaaa tcaaatactt tgttggtata
     9241 atcaaatcta aaaagcggat cgcctttggc agtaattaaa ataacaggac attgtatagc
     9301 cccattctcc aagcacgata agtcagcatt aaataagctg gcgacaaatc ttaaaggata
     9361 atagggtaaa agcatagggt catttaagca atcttgtttt gcctgttcac tataaaatac
     9421 tctgtcatag tctaaataag cgtctcctgg aaccttatag ttaggcagca attttccacc
     9481 tacacgaaag cccaactgta tcagccccat aaaacggtgt gaccatgcgg ggtattttgt
     9541 taagctcata gtttctttta atgtagtcag catgatatta tgtggaaaaa cggcttttaa
     9601 ccttgtgtcc ctacccgccg ccattgcagc taaaatccca ccttgactgg aacccatgac
     9661 agctatgttt tcaccaaaat tttctattcc gtaagtcgtt gcgtcatata cattatgcag
     9721 caaatcttcc attgtataat tttttttgat tttagggctt tttccgtgtg aaagatagtg
     9781 aatgcctatt atattaaatc ctctttcact caaaccacat agaaaattct catacatgag
     9841 agggtgtgac atagtacccg gcaaaaaaac aatggtagga ctatttcttt ctgtatacca
     9901 aatagaaagt gttatcggaa cattatttga agaaatacca acttctttat acataccaca
     9961 cgcctcctat atgactaatt tagttttata gtcatattat aacttattgc aagcagaaag
    10021 tcaatatcca taaatatatg atttaagata aaattgtcaa gaaagattta attgcttaaa
    10081 atacaatgaa atcgcttgtt agtgcagcac atttgctata gaaaaggcag actatatccg
    10141 aaaatagagc ctgccaacaa aatatcttac ataataattt tccagttgct atccttatgt
    10201 aacacaagct caaactgtga tacctgcatt gcctttgtct gattatcaat gaatttcacc
    10261 gcaaccctga ctttcacatt atccctgtca tccgtaaaca taggattgac taattcggaa
    10321 tagaggtagt ccctgccgat aggttcaagc acattgccag ccacatagta ggcaagttct
    10381 ttttctgttg ctgtcggata cagcttaaag aaagtttcca aaaatgcggt agcgtcattg
    10441 acggtatctg cgtccacgct tgcgtcggct tcttgtgcct ttggctcata gtctgatttt
    10501 tcaatcgctg gtgcaatggt tgggttctgt acgataacca tatcgccgtc tttatccaca
    10561 tggactttga cggtataggt tgctttgaca ttgcttgtct gttctccctc ttttatctgc
    10621 tgatctactt cgtaggtagc gataaaatcg ttcgttccag actgttttat atcccaaatc
    10681 agtacatttg taacagtgga gcttgtcggt atatccgtcc taatggtatc aaggttcaag
    10741 tcctgcaact cctttgtcag atagttgctg attgcctgcg tccttgcttc gatagcttcc
    10801 ttgctgttgt cccatgtgta gtaggactta cagaagttct tcacaaaatt ttcaactccg
    10861 ttggtgtcat ttagtcttaa ctgaatggtt tccgtttcat gtaccgtgtg catatcaatg
    10921 gcggtaaagt tcttatacac gccaaatgat acgctggcga taagcaccac ccacagagca
    10981 atcacagatt tcttatgtgt gcctactttc atagtgcgga ctttcgtttc ttttactggt
    11041 ttttctttct ttttaaacat ggtttccaat cctttctact gttttattct tccagcacaa
    11101 atcaagtact gttgccaata agcattgttt aaatcggcat atccgatagg gtcgcctgca
    11161 tgatacatct gagtgtttcc tacatagata ccgacgtggg ttacatagct tcctgcattg
    11221 taggtactgt ggaaaaagac caaatcgcct gcctttgcct gcgatagtgg gatatgctgg
    11281 gttgcgtcat actgattttg tgcggttctc ggtaaagata cgccagcttt gttgtagcac
    11341 cactgtacca gtcccgaaca atcaaaagaa gtgttcgggt tactgccacc atatacatac
    11401 ttccaacctt gatattttaa ggcttcattc ataacctttt gtgccagctc gcccgatact
    11461 tgattgacaa caaggtactg gttcacaagc tccacataga acatatttcc atagccataa
    11521 cgccagccac cattttttgc cacggcgata gggttcgtat aggttacttt ttgaccgccc
    11581 gacttatccc ttgcgaagtt ttccgcaagg ttgaacatat gcttttttcc gtttcctgcc
    11641 acatagccaa catagccacc gccatagtta taggactgta tcacaacatt tatatcactg
    11701 ataccttgat ttttgcagga agaaagcagg gacgcaaaat atttacaccc ctgcttgatt
    11761 gagctttccg tatctagtga gttgggtggt agtcccagac tttcagagct ttgcataacg
    11821 tcctcggctg taccgccact ttccacttgt atgattacca gcaacacatt gacatactcg
    11881 gaaataccgt tttctttggc atacttttct accataggct gatgtttcaa gacttcggca
    11941 gataggttca tacctacata attagaggaa taaccattgc ccccgtcgtc gtcctcggca
    12001 ctaatcagaa ccccaaagaa taatacgaga gaaaagagga tagaaaacag actgccaaca
    12061 atagcgatat gtcttagttt catttcttct tttgtccttt cttgaatacg gttcgtttgt
    12121 tcttctcggt attctgctgt gtatgcttct gaacggttgt tttcgtaaca cggtcagtct
    12181 tgtaattctg tcttgtggtt tcttgattga cggtctttgc tgatgtatcg ccagaagata
    12241 gtggacgttc cttgacaaca tttttcttga taggttcatc attcttcacg gttgaagcta
    12301 tggcagggcg tttgacttcc tgctgttttt ctacattaac cttagaagtg gtagctggtc
    12361 tttcatgggg acgggtagct ccgcttgtcg ctgatccgtc cgttttctta cctgcctgcc
    12421 ttgattcttg tgccttttga agttccatac gcttgtcagc gatattctgt ctatgtagct
    12481 gttgcttctt taagcgtcca gactggcggt tttccttttc ctgcaccacg ccacgcttga
    12541 aatcagacac actggacttc gccttttcct tagcagagtg aaccgcatag gcagtctgtg
    12601 tcggcatatc tttgatattt tctttgacag cagttgcctt gtccttgacc ttattctttg
    12661 tatcaaggac agctcccacg gctgaacctg ctttctgccc catgctggag cttgtatttc
    12721 cacgattgtt ctttgttcca ttctggcgtg tagattttga actggcaacg acaccaccag
    12781 ccaccgcacc agcgacacca ccagcactaa cagctcttgc aagcctatgt tccatacgtc
    12841 tagccctatg tcgcatgaac agatacggtc tgcggaatat tcgccgtccc atgctttgac
    12901 tgtcgccagc gtttaaagag aacatgctca ttaaatcgcc cagcttcatg tagatacctg
    12961 caaaatacac tatctgcaag aacgccacca taaagaacgg gtagtccgtc gatatgttgt
    13021 agaacatact ggaaatacta aaggctaccg tgacaatcag tgtaatacct gcccttgtca
    13081 taatcgtatt aaacacacgg actatggctt gttttgccat gctctcatag ctcggtatca
    13141 tacttaatag aaagctgata ggcagaaaca tagcgaaaat gataaaaagt atctggctga
    13201 acaacatcat acccgtaagc aagaatacaa atatcgtgat acccaagttg aagaacaaga
    13261 ggaagaacac catacctaaa cggtttacca cctgcagtat cgtcagattg ttgttgtcgt
    13321 tgtcctcgat ttctgttttc acgacttcct ctctggtctt gccgtcctcg tcctctgggc
    13381 ttgccgatac gagagcttcg acacggtctg ccccgatttc ctctataccc gaattaccaa
    13441 attgaagcag tagccacggc tgttgtactt gtatggaaaa taggctatcc cttatcaagt
    13501 ccacgctgtc cttgcccttg ctgtcgctgt cgggaagcat gatttttgtt cccaagtcca
    13561 gtgaagcggt actgatgtct gatgaaaagt cattcacttt cttgatgtag tcaggagcat
    13621 aggcgataaa agaagccgat aatacgaaca ccacaacaaa gttgataacg gcgtgtagtg
    13681 ccttactggt ttcccgtttt atcagtcccg tataggcaac atagatacct acgactaaga
    13741 taatcagcag aagaaatccc acataaaagc ccgaagtaga aaaaccattt tgagttacgc
    13801 ctgcaagggt ctgtatgctt cttccgatac tgtctgccat atcgttgata aagtccagct
    13861 tgtaggcttc ctgcactaca tagcccgttg cattgcttag atatagtctt atcgtccaga
    13921 caaagttggt aatacagtaa agcccatatt gcacactttt tccaatcccg tccagccagt
    13981 tccacggcaa ccacgcccag ttattatctg catagaaatc aagctgataa ttcgacagcg
    14041 gatatttgga ataaaggttc tcggtgttga tagtgtcgtc cacaagtccc gttgcgtgtg
    14101 ccaccgttcc caaaagtgaa agcaggataa gggaaagtgc cacgacaaac agagccattt
    14161 tgagaaagcg gaatatcttt tttcttgtga acacactttt tatcctttct ttcatcactc
    14221 cacctcattt cgctgtatcg gcggtctggt atcaaaggcg tgcagtaatt cttcaaagac
    14281 tgggtgtatc tgcaccacgc ccacacgtcc gtataaatct tgaagcaagc attgcccgtt
    14341 ttccaaatca cgcaatcgct tctggttgtt ctcgtcctct ttgtcaatgc tgaaaaattc
    14401 aagggtctgt tttatttcgt taatatcggt gcttctaaag gcaaatttca aaccgatatt
    14461 gtttttcaga ctttcctttg acacgtcgcc agaggactgt gtaacaaaat aaacgcctgc
    14521 gttcatggca cgtccagcac gaaccagctt gttagagagc gtttcgcctt gtgccacatt
    14581 taagaacgcc catgcttcgt caaggtctac aatcttgaaa acacttctat ccgagtggat
    14641 aaaatctaac gcaaaggtac taatcacaat cagcatggaa acggacagta attcaaaggt
    14701 cgtgtcctta tcgggtagca ctaagtcggc tacttgaatg atattgagct ggttgtctaa
    14761 gctgattgca ttttgcaccg taccgtccga gaacagcaga tgtgcaaagt cgtagtccgt
    14821 gaagctgtcg atatggtcgg caatattgca agagataggt gtatcttctt ttcttaattc
    14881 ctcaatcaca tggagcaatc ctcgctggtc gctctgggta acggcacgca ctgcctttct
    14941 aagtacgggg aacttctcgc cgtcccttga agaaatgccc gtaaggaatg tgagtatgtc
    15001 gattgccaga ctttcagcgt ctttcacatt ctgcataatc acgaacgggt caagaagccc
    15061 tgcattttct ctgtcgctgg taaggttcac gatattgatt tcatgggcga tttctgggag
    15121 cgtttctttc cagttgccac gctcggattt tgggtctaag attaccgctt gaccgccaaa
    15181 cagcacagag tagtagacaa gcaggttgtt acagaatgat tttccaccgc caagactttc
    15241 tacaaaggca gaagcaagag cattagtaac cgtatctttt acgccttgac tggcaaggga
    15301 cggttgtaga taaacattgc gtcccgtatc gacagaatag ccgatataga tacccgtgtt
    15361 ttcccctaac tgctgtgtcg caccaaagcc cagcccagct agaaaatcac ttttgacata
    15421 ctgcacataa tcgttgatgt atcgcttgct ggcaggaaga aattcagagt gaagccccag
    15481 catatcgcca gcagggcgta ccagcttgac gttcaaatcg tcgtagaagt ctttgacttc
    15541 atcacagcgt cttttcaact cgtcaaggtc tggagcagat acacggataa cataagagag
    15601 cttatacata ctttctctgg actggtctaa atccgtttcc agctcgtcca cactgtctaa
    15661 tgcgtctacc acatttgagc tggtttcact gccagactgg tatgcatgat tgtctaaatc
    15721 tttcagctct tttttcttgt tgcgaaccgt cgataacgct tttcggtttt ccacgatttc
    15781 tacattcatg ctggtatcaa cgggaaaagt aaactgctgt tgctggaaat agaagatttc
    15841 actggacgga aaatcaagct cgccgacaat cgcattgaca gtaaagtagg aaacgtagct
    15901 ttcgctgtcc tcatgttcca agcgtaaatg tcgctggctt tcttccacaa ggcaacgggt
    15961 cggacgtatc aagtcataat gcgttatcag cgtttccttt ttcagtttct tcttcggtaa
    16021 gctatactca taatcttcat aggcgacacc gtccctgccg tagatgtgtt caatcagata
    16081 gccgaagtca tttttatcta agcgtcttac tttgaagcgt cggcttattt tattttccaa
    16141 cagcttttcc attttcatgt agcggttgat ttcatcattt ggcatggaaa caaagtcatt
    16201 catcagcgtg tggttcactt catggataaa ctccttgaat gtgagaaacg ccgattgctt
    16261 cacgctcttt aggttcatgc tctggtctgt taccatgagc ttgaagccaa gaaaaaagcg
    16321 gtagtccact tgattgtccc caatcataga tactaacgct tcggtctgtt cgtctatctt
    16381 ctgataggca acgtctttta gtcgtcccgt aaccagcttc tttgactgtt cttgtatgct
    16441 tcggcttgag ctttctgtgg caatctgcaa agcgtgtatc ttgccctcac gggattgtgc
    16501 gattagctgt cggaagctgt catgcacaat gaatttctgt tcagcggata gaaaactgta
    16561 attgtaaggt atcagctcat agtaggcgaa cacctcattg tccttgttcc agacaaggtt
    16621 gttgtcgata tatttaatcg ggaacatagc tcacactcct tactgccgta atagcttcat
    16681 gtagtacctg cttatccagt ttcacggctt ttcctgcata agtaatcttt ggtcgtaggg
    16741 caaaggttat ctgtgatttc aaaaagctgt acggcttctt gccgtcaaag gtcttttgcg
    16801 acatgaacca tgtaagagct acgggaatac cgaagtattt taaaaatgcc ccctcaatca
    16861 tggataaggg cggtaagtcc ccaaacagaa tgatgataaa ctcggttatc acaaaccatg
    16921 taatctgtgt aaaggtaaca ggaaagggca ggttaaagtc attgattgca tacaagactt
    16981 tttccacgtt ccagataccc gtgtagcttt taatcttttt cacgtttttg tttcaactcc
    17041 tttcgtataa tagaaaagga cagccgtttt tcagactgtc cccaatagaa aaagctgtca
    17101 gaagcaacaa tgcttgtcgc tgatcttcca gcttcttacc acggtatctc acacataccg
    17161 tatcttgttt caataaaagt tccctcaaag gataaatccc tgccgtatgc ttcatagtca
    17221 atatagttct gcaaactagg gggaatatcg ccaagtgcct gcaattcatc aatgaagtaa
    17281 taggcaacgt cggtcatatc ctcacagcca gcgtataaaa tcatatcgtc cctatggtcg
    17341 tacagttcct ccaaacttcc ataatttgat acaaattcgt ccaaactgtc cgtgatgtag
    17401 tcgggaagct cacatatcat ttcatacatt tcattgagct cttcaatcga gatatattcg
    17461 ccaatctcaa tgggaaagtt ttccgtatca tgtacggcgt attcttcgta ccgttcattc
    17521 aagccgatac gctcggcaac atcttcttcg tcaatcggaa aagagaacca gtcgccgacc
    17581 agatacccct cattgtattt cccaagatta gcgatatata cacgcatatc ctctaccata
    17641 gaaacacctc acttctacgg cagttcatag ataccgtggt ctgtttctat aaattctccg
    17701 ttatcatcaa gatactgtcc gtaggcttca taatcaaagt aacgggtcag gcgttcctcg
    17761 atggcagaaa aagcagggtc attgtccaac aaatgccttg ccacatcagc catgttttta
    17821 caccatgaat aatgtatcac atcatggcgg tgctggtgca gttcgtcaag gctggtaaag
    17881 taacacatca gctccccgta gtcctcttgt aaatcggcag gcagactttc aaaggtatgg
    17941 taaaggtcat tcagtttttc aatggtcgca tcttcgccga cttcatcagc aaagggcagt
    18001 tccttttcaa cgatatggta atcttcactg tcgataggaa cgcctatctg ttcttccagt
    18061 tcttcctcgt caatgggaag tgtgaaccat gcggaacgga tttcttcaga agtgtgtagt
    18121 cggctttcga tacagacacg catttcattc ataggttttc acgtcctttc tttcatgctt
    18181 cagctatgct agtgtaaggg acaccaaaga catagcctgc aaaatcttct aattgtttct
    18241 gcggataatc ggcaaggttc agtcgcttca tttcctgcca caccatacgt ttatgatgtg
    18301 gtaaatctga aatgtaggta cagccgatac ttgcctgcat atccgaaaat atatctttca
    18361 agcaatcact ccaatcttaa atttaggtat gaaaaaaagc agtcaatcgt atagactaac
    18421 tgctttgtat atagctgtta aattgagttt atcaattctt gtacttcatt aaaaaatctg
    18481 gataaatgga atccatctgc tacggcatga tgtatattca ttgtaagagg cattatatat
    18541 ctttcttgct ctaattcata tttgccccaa gtaataactg gtgcaagaaa ttttccctcg
    18601 tcaaaaacat gaacatcaaa atgattatat tttacccacg gtaaacaaga tacatcaaaa
    18661 aagttcaaag attgattttc cacaaatgca cgttctgttt tatacttctc cctatctaat
    18721 ataaaacgtt tatgaaattc aaatatgcta tcagaaaaat ctgtaactat ctttgtgaaa
    18781 ttttcatctt ctttatgaaa ttcagtatag gacggagaaa tataatccca cttaattaaa
    18841 ttcccattat tatcccaact atatttgaac tcatcatgtt cattaacaac ctttgaaaca
    18901 gcccatatca tagctggata aaattttaaa tcatgcttct ttgaaaattc aataaacttt
    18961 gttacatcaa tatctacggt taagctcata acaatacgca tattatcaat gtagctttga
    19021 aacaatttac ctctattcca tttattcaaa tcaatttttg tatatttata ttccatgccc
    19081 attctccttt acattttgaa atttgataaa atgtaaggct ctctggataa catttcactc
    19141 catacaagtc tattccatta agccttatat ggagataaaa caatacacaa tttcacaata
    19201 tcactcctta tcaaaaaaac tttcgtttat tattttcata tctttattat atcaactatg
    19261 tttgagccaa gcaatgaaat tcattatgct gtatttttat ctgtgtacca ttccgttacc
    19321 ccctttcccc ttagcccact cccaaagtgg gaaaggtatg tttgcgtatc cgcaaaccat
    19381 aatgaaaaag tacggctatg cgccgataat cttattgaat agttctaaga gtacgtcttt
    19441 gacaccgcct gcattgaata ccaatccaac agcaactaag gcaaccacca agaagccgat
    19501 aagttttgaa aactcacgtt taaaccctaa gtagatacca ataaccacaa tcgccataag
    19561 cactaagctc tgggcgttgc ttaaaaacca tgtatataaa ttttgcccga aattcattta
    19621 tttttcctcg ctttctttta attcaatcat gtgtcggtca gcttcctttc cgacactcat
    19681 aatacacatc agaaccacgc cgatacccat gccaagtgat accagcagta agtccttaat
    19741 caattcccac atcatcaatc actccttttc tttttctatc agttcgtcca ccgaagccgt
    19801 ctgctgtctg attatctgct tgtgcttatc tgtcagcttt gcctgctgtt caatggtctt
    19861 gagatagtcc atctgattgc cagcgtctat ctgtttcagc attttcaagg tcggtgcaac
    19921 ctgtctttgt aaccagttca gcgtcctttg cagagtgtaa ggctctggct ttgtcgtcag
    19981 cttcacgggc ggtcgtccct cgccaatgaa ccaagaccag cgggcgttgg ttttccagtc
    20041 gctttttcgc ttgttggctt ccttatcggc aaagcggata tactggttga taatagaaaa
    20101 tgccgtcagc tctgcgtcgt agtgggtcag cagctctcta atggcatagt aggcgtgctc
    20161 atttttcaaa cgtatctcaa atcggttttt aatggggact tcctcaatcg gtatccctaa
    20221 ttttgaggat tgttcataat tcttttcata gacacagaag tacacttcac tggtaaatga
    20281 accaatgtaa agcgtatgtc ccatgccgtc cttgttggct tcgttgtgct ttaccagctc
    20341 cccagaacca tagttcttga agctacggaa tttagaaata cattcctcgt tcttgcattt
    20401 ctctatcagt tctggaatat ccagtaagcc cgttctgtcg ttgatagcta ggtcgatacg
    20461 cttcatcaca ccgccatcaa ctaatgcgtc catgaggaag tcataccagc ttctttcctg
    20521 tgccagcaga tagctttcaa actgacgaca gccttttcct ttcagttcca gcaggacacc
    20581 tttttccttg tcttgtgaag tgtaaacgaa cacgtccccg atatagtaat gctcggtgta
    20641 cttgtaatgc ccgaaatctt cgtgaagcat ataatctata ttcagcttta gaatgtcctt
    20701 gataacgtgc tgtatatcca gcgtgggaaa tcggatacgc acataatcaa agagcaggaa
    20761 catgggagcg tcgggattga atttttccac cgcttccagt agtttgtgct tcatatcctc
    20821 ggtcagtgct accttcccag cttcgatacg gcttagatgt tcacggctga taccagccat
    20881 gattgcaagc cttgtctgcg atacgccata atcactgcgt tttgctttca aggtgttcag
    20941 aaaatcagta tcattcagtt gtcatacccc caatcgtgat attgttgtga ttttgtgata
    21001 ttttcaagtg attatcacaa ctagctaaaa actaagaaaa tccttattcc tagcggactt
    21061 ttcaaggttt ttgaaccagt ttccagcgta attgtgcccc tctgttagat aatgaggggc
    21121 ttatatctgc tggcgtggct aacgccacac cagcaacggc tagtccgtgc cgtcgccttt
    21181 cgcttcgcac gtcgccgtcc cgtcctgcct tacgaatgca agagagccta tggtctgcaa
    21241 aaaatcatgt cctttcggga ctaaaggcgt gtaaaactca cttatgacac ttgttcccac
    21301 atcacaatag ccacgcccct tgatacgctt ctggaaaaac tgttttttca catcagaacc
    21361 aaagagcata ccgtaaccca attcgctgat acgccccaat ccaacacgga aattgaaatt
    21421 atctctgata ccgtccgaga aatattttgc gtctggacgc tggcaggcaa caataaggaa
    21481 atatccagct tgtcgtccca acataacgat tttctttaac tggctaagta aacttacgct
    21541 ttcctttgtc cccagcattt caaaaaacgc cacatattca tcaaagataa gaaagcaggg
    21601 cggtaaacca agataggcgt agttttcgcc cgttttatag tctgggtgtt gcttcatttc
    21661 ttcgcttctt tgtaccatgc cctcataaaa ggcattgaca caatcaatca tatcttcttt
    21721 ggtatgatac acatttccca taactgtacc taagtctgca aggtcagcgt tctttgggtc
    21781 taagatgtaa aggacagcat tcgtatgtag cagagcttca ataagcgtca gcagaaagta
    21841 ggtcttaccg ccaccagtcc caccagctat cagagcgtga gggagtacgt catattccca
    21901 gacaagattt ttcatcagcc taagacagcc gttttctgct ttcacttcgt caatggaaat
    21961 acggttcgct atcatgtcat ataaaagcgt gtactcaata tagccgtcat gcaaggtctt
    22021 gtcggtcagc tcacagtaca aaccgctttc cagcttatcc tctaatcgca aaagctggtc
    22081 ttgatatttt cctaatgtga tttcacagtg aatatgaagc aatccgtttt ccatttgata
    22141 ataaatcttt ggaaaccaga cgattttttc ccttgatctg ctttgcaggt cagtaaaaaa
    22201 accgctgtct tgtacggtat ctgcttcata ccacttgttt tccagtatca ttcgtgccag
    22261 cttttgacgg tgcaggagct ttttgaaact gtcataacag aagcggtaat acagaaaagc
    22321 gaccaatgca caaataccag tcgctatcaa tatggttatg aagttgtagg gggaaagcgt
    22381 caagccattg tccaataagc tgaaatgttc ccagtcggta cgcatgagct gtttgatatt
    22441 cagtagcagg agaaccgcca caaatacaaa cagaagcgtc cctatggaaa agtggtagac
    22501 aaggttcttg tctctggctc tgatacggtg tcctttattc aaaatcattc gcataaatca
    22561 atcactccaa tcttaattta agtatgaaaa aagcagtcaa tcttaaaaga ctaaccgctt
    22621 tgtgtgtatg aatatgaagt tgttacattg tagggtatgt tcattatata tgctttgttg
    22681 ttttaactct tgattgtgca atttcttcaa atagctgttt ttcgtttagg tctttaaaaa
    22741 ttcttttggg aattaacgta tataatcgtg gtgcttgaat taagtaataa aaatcatggc
    22801 tttcccatag tgcagagtaa atgtcccact tcacttctga gtcaatgctt tgtgttttaa
    22861 atttaatgtt ttccttagaa aataccagcg tatattcctc atgatatttt gcagtttgtt
    22921 tgaatttcag tataggcatt aggatatata aatagcttcc caatgcagtt acaacaatta
    22981 tcaatccaaa tattaaaata ctaaatacgc taaaagagga gaacagcata taaacgactg
    23041 acagcaacaa aaatacagcg actaaagcaa tatcatattt atgtatagtt ttactagaaa
    23101 ttaaatattg cctttctgct tttatatatt cactttgagt atattgaaat cttaactcta
    23161 ctgtttccaa aggtattccc ccattcgtca tataaataat ctttgttaca atttcaaatt
    23221 tgaaaatcac tttttcttga aaagtttcag ttttatattt ctcctttcag caatcttctg
    23281 aaacacctcc accacataca agcattgtgc cttaaatggt agtaaggtca attcgtcatt
    23341 ggattgtatt tgtttcattg cttcttgtaa ttctgaaagt tctaataaat tgactgccac
    23401 acaaaagaaa gtctttctac gcccgtcatt atagttagaa agtaagtaag aaagaatttg
    23461 tgttttttcc tgctgttcaa gattatattg ctcaatgcct atgttttttg ctctttctaa
    23521 atctgctttt cgccgtttat gcgtgatgaa agaatcataa tcatcaatgt gttgatattt
    23581 ttcgcatgga tattgtttgc attcatagca atattcaatc tgtccatgtt caagactaca
    23641 ttttgcaatt ctgcatgatt gattaccatt gccacaaccg ccacagtaat ttcccaaaag
    23701 catagggcat agtccgcaat tcaatccgca aagagaaaat aattgatttt tgcgttcaaa
    23761 tcctttcata gtattagtct cctaactttc aatttatcgc atacaagcat ttataaattt
    23821 cataggtaca tccatacgtt ctgcaacttg ttctggtgtc attccactat ttaagaaacg
    23881 cttgcacact atcttcatgt tttctccaac ttcgataata tgtttatctg ggtcataaaa
    23941 gcggactaca cgctgtcccc atgaatgttc tataataggg tggacatatt caatatcata
    24001 ttctttcagc ctatcagcaa atttatcaaa atcttcttct tcaaaataga cttcaaaatt
    24061 gttaccacca aaagaaatat cacttgtacc gataaactct ttgtatgttt cacttgtctg
    24121 taaggctaat ccacctgtta aagttttatt tgccccaaaa tccataatga cataaagtcc
    24181 aaaaaccttt ttataaaact caactgattt atctatatca gttactacca acatagggtt
    24241 ttttaatttc atttttgcta cctccacaac ttccaatttt ctttatgtcc acatgacagc
    24301 attttcatat atccgctatt ataagaaact atccatttgc taatggtgtg gtaataaact
    24361 actttctatt tggttttctt tttatcctca aaagtttaac aaaatcgtca aacaaagttg
    24421 tatctgttgg ctcaaacatc agccattttc cgtcatggta agtttttgtt tcatcataaa
    24481 ttctctgaac ttcttttgaa taattatctc tatatgtatc gaattttaac cgttcatctt
    24541 tacctaagat aatcataaat cccacacaat tttctctagc atataaggca cataatgttt
    24601 tgccacctcg acgatactta tattcataat tccatgcttt gccaccttta ttccatagac
    24661 attccatttc atacttttca tcaatcaagg cacacaattc attccataca ctatataatg
    24721 attgtccgac caaagcagtc atttcctctg cattaggtat tttatctagc atacacattt
    24781 ctcctttata agtctaaatt aactgtttcc acatccatat tataacagtt gtttatatct
    24841 cctacaatga aaaactagtt tgtcagctta ctttttctgc tgtccctgcg gagcgttgtt
    24901 ctgcggattt ccagcttgac cgcctttgtt cttcaataca atatcatcag ctttgatgta
    24961 ccaatcaacc gtagtccctc taaagttggc atttgccacc gtatcagcta cgggattgat
    25021 taactccact tccgcattat aaggaaagtc ctttacggga acatcagcgg ggattgacac
    25081 ttgtatcatg cgttcctgca cactacattt caaatcataa gtacgacgct taatttcatc
    25141 acttgtcgtt ccgtcctcat tttctacacg cacctcatgt cgaagtgcag agaatttcaa
    25201 tactccaaaa gttttttcgt tatctaatac aattccattt gttaatctca taatcaaata
    25261 cccccttatt tgctttctgc tggtaacata tcatcagcca ttaaaatgta gtttgtaaat
    25321 ccacgtcctt tgacgttgta accctctgcg gtaatgcgtg ggttaatcag ttttactttc
    25381 tgctctggct taaaatgctt ctcgccagct tttgcaggca atacaacgat aatgtcgtcg
    25441 gctctctgaa tatccgaata caagttgtaa cttcttgata cgagagtgta acgcccattg
    25501 atacgttgct ggtctacttt gttttcccct gcaaattcca agtttccaaa agtctgtgcc
    25561 acattcggca cgatatattt taattccata ttattttacc tcgtttctta ctttaagttt
    25621 gattgatgtg tgtattctgg catttaggaa agtgccagca agcccccaga tagtaaaggg
    25681 taaataaatg ctaacgcacc cttgactatc cgtgttcttg caggtaatcg gcagacaagc
    25741 cagaataggt atagtctgcc tatctaagct atggcggaga gcgtgatttt tcttttctca
    25801 tacagctacc gccttatgat ttcttcatct tacggtgttt gaaaaagtat gtaccagcca
    25861 gcccaccagc agaaacaaac atcataattg caaaggcaag gatatttgta ttgtcgcccg
    25921 ttttgggact gtctgttttc gtaggtgtat ctggtgtggt tggcttctct ggttcttctg
    25981 gggtaggtgt ttctggtact tccttgatag taactgtctg tccgtcgtcc tcaatgtcct
    26041 tgtgttccgc aactttgata ggttcgtctg gattgcttaa atcgtataat tcctcaaaag
    26101 ttacaagctg tttaccgcca agattggaag cgtcaaaggt aaactcgatt ttaacttcca
    26161 tagtttcgct gtcagccgta aagatgtagt cattctcaat acgcttgtca ttgatgataa
    26221 gctctgcatt ttcttctttt aacatctgcc agcctttcaa ctgatatttt gtccctttgg
    26281 taagaccgtc taatttaact gtatcagtga tagttacgtc tttcccagca agaatagtct
    26341 tttcgccgtc cttgctggta gcagtagtat ggatagtgat ttctttttcg tattcatcag
    26401 tcagcgtacc taaatcaacc acaaccttat ttctggaaat gacaatctca aacggtggaa
    26461 taagtttgaa acctttgttg ctttcacaac gtaattcttc aatgatgtaa gtatcataaa
    26521 gcaacgcacc tttgctgtcg tctggttcag aagtaccaaa ccacacaccg tcctcgcttg
    26581 tttttcctgc attggtattg tgcttgtggg aagcccaatc agaagcagta gagaattgtc
    26641 cgttatcatc agttacaaca acatgacttt cgcccgtcgt cttgcttgtg atcctaaacg
    26701 gaacatctgc aagacgcttg tgcgaacctg caccaatttt tacgccctca atatcgccac
    26761 gtttaatctg gttatagata gagtgggctt cgtcagtcaa atcaacgatt tttccatttt
    26821 ctgtgatttc aaaatcaatc ggctttgcac cgtcagtcaa atatccctct ggcggttttt
    26881 gttcagacaa tctgtatttt ccgtaaggta ataggtcagc agaagtagaa gcgataccct
    26941 caatgtcggt atggattgtc tttaacactt catttttctt gtataacttg ccctcaacta
    27001 agacggcatt ctcatttaag gaaatgattt caaaggcggt atctttcaaa gtcgcaccgc
    27061 cttgagcttt cgtgtctttg gtttctaaat ctcgtttctg gattttcaca ccgccacgaa
    27121 tgactttatc agaaacatgg tattgattgc ttccagataa tacggcaagg tcgccgtcct
    27181 ctgtaatctg tgtcagatac attcctttta tctgttcttc gctaccgtca gcctgcatat
    27241 atgtaccctc taacaagtaa cccgacggac tttttgtttc ctcaacagtt agtgttccta
    27301 atggaagtac gtttttaccg tcctgcgtat agaagctgtc ccaagatact ttatatttgt
    27361 cagctaaacg agtaatgtaa tggatagcac cgtcgctgtc tttttcagcg atagtctttg
    27421 ttacccatgt tcttgtaggc tgtgctggaa gattgttttt gttgtaatat ccagcgtaga
    27481 agttccatgt aaactctgct ccctctaaag aagcgtttcc ctgcggattg gatttctgtg
    27541 tttccatgtc aatcttgaat agctcaatca aggtatctgt tacttttggt gtatcagata
    27601 cttttaaagt cgctgttttt ccaacttcca cagataaaga gtaaactgtt ttatcaattt
    27661 taaatcctgc tggtgcagaa agttccttga tatatactat agtggctctt acttcaacgg
    27721 cttctgtatt tccgctagta tcagtcgtaa gggtggtaag ctgtttcgtg cagtctttat
    27781 cagaatacac accgtatgtt gcaccagcga ttgaataaat cccgttgccg tctgtaatat
    27841 tggcattggt ggaagtcttt tgaagtttgg tatttccgac agctaacttc gcccagaatt
    27901 gccctaactc ttgaccctcg ccagagtaga tatatccgcc acagtcataa cgtcctttgt
    27961 tttctttcac aaaggctttt gctcctgcga atacttcatc ttgtacggct tttgggattt
    28021 catcatagga agctcgcaca ttatcccatt gccagtcagg ctgaacactt aatctctgcc
    28081 agacaacaag ctgtcttaat aggtaggcgt gtttactgct tattccgctg tggatgtctg
    28141 tgtactgttt cacatactca atagataagg cgacgtcagc tatctggtcg gcactcatac
    28201 gggtacttgc gtcagctttg gtcttatagc cgtttttaaa ggctgtgttg atgtcgatac
    28261 aataagcgtc ctcaccctca acggttaaat gtccctcatt aaaggtagaa ctgatagaac
    28321 cgtcattcat tacttgttct acgatactga cacgctcttt actttccgtc caatactgtg
    28381 tattagacgc atgaaccgtt gaactcggta aagcggtaac gacagttgca aaggcaagaa
    28441 agcctgtaca caatcgtttt aatatctttt tcataattct aaatgctcct ttcattttgg
    28501 tagtaaaata gccgtccact aggaacggct ggaaatagaa aaggaacgct gatattgtgc
    28561 gttccgtagt ctatgaagta ttcaattttt cttgtttcaa tactgatgtg ctgtaccgta
    28621 tctttgcata tctgggaaat aggacgtatc aaggctcatt caatcgtagt aaattagtag
    28681 tattttgatg tgtttagctc cttgataatg ccgatagata cagtgtttta gcggataatc
    28741 aaatttttag tgtgtttttc tgttaatagt tttctaaaga tgctctattt ataaattttt
    28801 atcaaagatt ttataaataa ttgtatttaa tatacaaaat atatataaat attgatgttt
    28861 tatatataaa gttgtatatt tttttaagga tataaagtaa aaaaatatac agtatcaaga
    28921 aaaaaagata ttgggtattt aaagtaatac tttatttaat tttaagtaaa tcgggtatct
    28981 acatatatag atactaattt caaagaatag gagggtattt atatggaaat attcgcaata
    29041 ggacattcaa attatccata tgataagctt attaatatga taaaaaaata tgacataaat
    29101 tgtgtagttg acattagaga aactccatat tctaaatata atattcaata caataaagag
    29161 gcatttaacg aatctcttag aaattccgga tttatatata tatacatggg aaaagaattt
    29221 ggagctaaaa gaaataataa agatgtatat acgcaagaag gttatgcaga ttttgagaaa
    29281 gtagcaaaag aagatatttt tttaaacgga atagaaagat taaaaaaggg ttgtcaaatg
    29341 ggttatagga tagttttatt aggagctatg caagaaccta taagatgtca tagaagtatt
    29401 ttggtgggaa aagtattaaa taaagaaggt ttcgatgtaa aatatataat gcatgaagga
    29461 aatttggcat atcaggaaga tatagaggaa tcattattgg ataagtattt tagtgataga
    29521 gagcaactga gtattgacaa tttattaggg agtgcattaa cgagagaaga aatgatacaa
    29581 gaaggttata agctagctaa taaagaaata ggatatagaa ctgagaaact aggtaaataa
    29641 aaatatacaa gtagataaaa tacagtactt atcttaagta aaataatatt atgaatttat
    29701 aatagctagg catgatgtgc caattaaaaa tattctagtt atttaataat atcattaaat
    29761 ttagggtatt ttttaatagc acatcatgtt gcagttttaa aaaataaaat ctctaatatt
    29821 gccataatca actgttctat taagagtagg atgactagat ataaatctaa ctgtaatttt
    29881 tgaatttaac aaacaaggat ttgaaattaa aaatactcta tctccattga caatagaagt
    29941 aatagctact ggcaaaatat ctacaaatgt atcatatctg tttttagcaa tttgataagg
    30001 ataatttcct acaaaaacaa atagatttaa atcatatata ttatcagtaa accaagccca
    30061 ttgaccataa actttgtcga tattcattgt gcctaaacca agtgtatcaa agtatgttgc
    30121 tatagttaaa taaatatttt ccatgttttc taaatatgaa acagtatatc ttctgaaacg
    30181 tatgggtgct gtggaagaaa taggataaac catatttaca tttagattat ttgcatttaa
    30241 attaaccaaa gtatcacatc ctaaaattag tctataaaat acaatattta aatggatgcg
    30301 attttgttac atctaaagtg taatatttta tatattgaaa aagtggtaaa agtttggatg
    30361 attaaataat attataaaaa atatataaaa tgtacttatt gttattgcta cacgatagtg
    30421 atacaatatt aacgaaatat agctactttt aataataacg gaaaatattt atattaaatt
    30481 aataaatatt ttaaatgtgg tatccataaa aaataataaa cataagataa ttataaagat
    30541 atactgtgtt caaatgatat taaacaagat ttatattatt tgaaagcagt atattataat
    30601 gcgaacaata caattctaaa ctttgcattg tcaatttaca gttcgataaa ctaattaaaa
    30661 attaaattta tataaagagg gaaaagagga atagggatgt ttataaggaa aaatgttata
    30721 aagttagcaa ttcctataat gatagagcag acgtttgtca tgcttttagg agtatttaat
    30781 acaataatgg caggtcatat tggggaagaa gctgtatctg cagttggaat ggttgattca
    30841 ataaacaata tatttatatc tgtatttgct gcattatctg ttggagctac tgtcgttgta
    30901 gctcaacaaa taggaaaaaa agatagtgaa aaggtgaatg aaactattaa gcaggctcta
    30961 gtgtctggag taatattatc ctttacaata acaatattga tgtgggtttt tagaactggt
    31021 gtaattaatt tattttatgg ttcagcagaa gaattggtaa aatcaaatgc aaaattatac
    31081 atagagttta cattatttac gtatccattt attgcgacac aacaaatagc aaatggtatt
    31141 ttaagagggt gtggagatgt aaagaaacct atgtatgtga ctatgtttat gaatatagta
    31201 aatgtaacat taggatatat acttatatat ggtattgatt taaatcctct aggaataaat
    31261 ttgcaaacac catcctatgg cataacaggt gcagctatat ctattgctat agctagaata
    31321 attggatgta tagtaattat aagagcttta tttaaaggaa atgaatttat atctatgggt
    31381 aaaatatttc catttaagat tgatgttgaa actcaaaagt ctatatttaa tatagggata
    31441 ccagctggag ttgaacaact actttttaat gcaggtaaat tgattgttca agtatttata
    31501 gttactatgg gaacatcagc aattgcatcc aatgcaatag gaatgtcaat tgcatccata
    31561 ataaatgtaa ttgggaatgc tttagctttg tctgctacaa ctttggtggg acagtatata
    31621 ggacgtggag atattaaagg agctaagagt actttattat atttaactaa atttgcaaca
    31681 atttgtttat ttatagtagg agctatattt ataccaattg caccctggat ttcaagtatg
    31741 tatactcaaa atgctagtgt aattgacatc tcaacacaat taattcgaag tgattgttta
    31801 gctatggtaa tatggcctat atcctttgta ttatcagcag ggctaaaggg agccggtgat
    31861 actagatata caatgatgac tgcagtaata ggtatgtgga tttttagaat ttttacaggt
    31921 tacgtattgg gggttgtatt agaatttgga gtgttaggaa tttggatagg tatgtatact
    31981 gattggcttg ttagagggac tttatattgt ttaagattaa gaggaacaaa gtggctaaaa
    32041 cacaaagttg catagaatat atgaaatcac ttttagatgg ataactctat ttagaagtga
    32101 ttttttatgt agttattatc ataattataa gtatgatata taaggttatt aaattacata
    32161 aataaaggct ataatagtat ggatattata aattatatat tgagcagaat tacataattt
    32221 ggcgataaat attagaaata tatatctata gaaaataata tatatgggta tattattaaa
    32281 tataaaaaat attaggtatt tatttaataa taaaggtggg tattgttatg ataaataaaa
    32341 taggaaaaat aactctctat gtaaataatc aagaggatgc aaagttgttt tggacagaaa
    32401 aattgaactt tgtagtaact tatgaaaagg aaatgggtcc aaatatgaaa tgggtggaag
    32461 tagcaccaag taaaaatgaa tttacaacat ttgtgttgta tgataaaaat ctaatgatga
    32521 agcaaaacaa agagataaat gtatctcatc catcaataat tttaagtaca agagatatta
    32581 atggaactta tgaacaaatg gcaaataacg aggttgaagt tggggaatta atggagatgc
    32641 catatggaag gatgttttct tttaaagacc aagatggaaa tgattattta gtcagagagg
    32701 ataaataatt ttacaaagta ttaaatttac tcaatatctg atataattag agacaataat
    32761 tattataaac tagtctagga ttagaattat ataattaaaa attaagtgta aattttgagt
    32821 agaggtgata aaatgtttaa gaaaatggca gtactaaaag atatagcaac taaaataggt
    32881 cgtaaaaaag cgtatgaact attagaaatg gttgaaggta atgatgcctt tgtagctgag
    32941 gtaaagataa aaaagaatgg aatagaatct aaaaaagaag aaattatgtt aaaagataat
    33001 caaaaaataa tattagagta tatagaaggt taaattttaa atacatataa gttgatatac
    33061 taaatttctg agatgagttt acataaaact catctttttt acttttcaat aactttgttt
    33121 tattatttaa tatacatttt atctgcttag agttgctgat atataaaaat tactgatata
    33181 ataaaagaat ggatattaat gtaatatttt atagaaaaga ggggtattat gcttgaaata
    33241 aaaaatcttt cctttaatgt tgagtctaac aatgaggaac taggaataat aaatgatgtt
    33301 agcctttcat ttgagagggg aaaacttatt gttatcacag gtccaaatgg tggaggaaaa
    33361 tctacaattg ctaagcttat tatgggaatt gaaaaagcta cgagtggtca gataatttta
    33421 gatggagaag atataacaaa tcttagtatt acagaaagag caaaaaaagg aataggatac
    33481 gcatttcaac aaccaccaag aataaaaggt atgacagttg aaaatttact tacacttgct
    33541 catggaaaac cattgtctac tgatgtatgt tgccaatacc ttacagatgt tggattgtgt
    33601 tcaaaagact atttaaatcg tgaagttgat aactctcttt ctggagggga aatgaaaaga
    33661 attgagatag caactctttt tgctagagat ttaaaggttt ctatatttga tgaacctgaa
    33721 gcagggatag acctttggag ctttggaaaa ctcaatgaaa gttttaagaa aatacatgaa
    33781 gagtctaatc agactataat cataatttct caccaagaaa gaatattaga gttagctgat
    33841 gaaataattg ttctacaaga tggttcagta aaaagccatg gaacaaaaga ggctatatta
    33901 cctgaaatca tgtgtcaagt taattcaagt tgtgaattaa tgaaagacat gaattaatta
    33961 gaaaggaagg caaattaaat gaacaaaata gatttaaatc ttttagaaaa agttgctgaa
    34021 cttcgtggaa ctccattagg agcatacaat atacgtaaaa atgggcaagg agtagagaga
    34081 cattcaactg aaaatgtaac tataattcct aaaactgata aacctggaat agatattata
    34141 gtaaagccaa atacaaaagg agaaaatgta catataccag taatagtaac agaggctggt
    34201 gtacaggata tggtatataa tgattttgaa attggagaaa atgcagatgt tgttataatt
    34261 gctgggtgtg gtatacataa ctgtggttgt gatgatagtg aacataatgg tatacataga
    34321 tttttcttac gtaaaggagc tcgcctaaaa tatgtagaaa aacattatgg tgaaggtgat
    34381 ggagaaggaa agagaatttt aaatccagtt actattgtca atatggatga ggattcatat
    34441 tgtgaaatgg aaacaattca aattaagggt gtagattcaa ctaaaagaga aatggatgca
    34501 aacctaggta aaggagctaa aatagttgta tttgagaaat tattaactca tggtaatcaa
    34561 gttgcagaat ctaatatgaa ggttatctta gagggtgaaa actcttctgc tcaagtagtt
    34621 tctcgtacag ttgggcaaga taattctaag caaaagttta atccttgtgt tattggggat
    34681 tccctttgtt ctgcacatgt tcaatgtgat tcaataatta tgggaaattc tcaaattagt
    34741 gctatacctg aaattgctgc aaatcatcca gatgctcttt tagtacatga agcagctatt
    34801 ggaaagattg caggagacca aatactaaag atgatgacat taggtcttac tgaagaagaa
    34861 gccgagcaac aaatacttaa ttgcttctta aagtaaatta aagattttat ttaatttaat
    34921 ataattaata tagtataata tagtgaataa gtctaaaata tttttatttt taggcttatt
    34981 ttctttatgt aagaaaagct attagagaaa ttatattgta attaggatgt ggaataaatg
    35041 aaaaatttac ttataagaaa tgggacaata atatctccaa cagatggtat caaatttgaa
    35101 aaaagagata tcttggtttt acatgggaaa atatatgcta ttggattgaa ccttaaagag
    35161 aaaataagaa aagaaaaatt acttacaaat aaagagttcg agataataaa tgccgataat
    35221 atgtatattt ccccaggatt tatagatgtt cacacccatt gttataagag aaaattacct
    35281 acaggtatgg attctgattc aattggaatt gaaaaaggtt ctacagttat atttgatgca
    35341 ggtacagctg gtccctcaaa ctactatgat tttaaaaaaa agtatatgga tgagtgtaaa
    35401 acagaagtat attctttttt aaatgttaca aatgagggac taaatatatt gaatgaatca
    35461 acttctttag atgttataaa ttttgacaag gttgagtcaa ttgtagaaaa aaatatagaa
    35521 aagataaagg gtataaaagt aaaagcgagt aaatttcaat ctaatgaaag tggtatagat
    35581 ataattaaga aatcaaagca agttgctaaa aaattaaatt tacccttagt ggtatatata
    35641 ggagagtatc catcatatat agatgaagta atgaatattt tgggaaaagg agatgtggtt
    35701 acaccagtat atggaaatag tacaaatgga atccttaaag aatctggcac attaagaaaa
    35761 tcagtaataa atgcaaagaa tagaggagta ttgttcgatg tatgtcatgg agtggaccaa
    35821 tttgatttta aagtttttaa aaaagcaata aagcaaggtt tagagcctga cctaataagt
    35881 actgatatgc atataaaaaa catgaaagac gtaaactatt ctcttttaaa tgttataaat
    35941 aaaattatgg aacttgggat atccctagaa aagtgtatag aaaaagtaac tgaataccca
    36001 acaaagattt tcgatttaca tggaaataaa ggcagaataa agattggagg agaaggtgat
    36061 tttacaatat tcacaatgga ggagtgtaat gatattataa aagactacaa taataatgaa
    36121 cttgttttaa ataaaaaatt aagattgcaa tatactgtaa aatcatggat gaaaagcagt
    36181 gaagtttata gacatggata cgaaaatact ataataaact aaacaatagt gataaaataa
    36241 taaatatatg tttaaattgg tatatattgg ataaatatct aaggaaacaa caattaaaaa
    36301 ataataattt taggggaggt attttagtat gaaagcattt gttgataagg atgtatgtgt
    36361 agcttgtgga gcatgtatag gaacatgtcc agacgttttt agtatgagcg atgaagggca
    36421 ttcagtagca attgaaggag aaataccagc agatttacaa gatttatgta aagaagccga
    36481 agaaggatgt ccagtttcag ctataacagt taaatagagc tgtttaaatt tatttttaaa
    36541 ataaatatat aagatttaat tgggtactta agctatagta ttagaaattt ctattactat
    36601 agctttttaa aatagataaa cttaatatgt aagatattac tttttacaaa tatggaggac
    36661 tgaaatatga aaaaaaatta tgaaatagta ttattcgatt tagatggaac acttacagac
    36721 cctaaagaag gaataacaaa gtctattcag tattctttaa atagctttgg cataaaagaa
    36781 gatttggaaa acttagacca atttatagga ccaccattac atgatacttt caaggagtat
    36841 tataaatttg aagataaaaa agctaaagaa gctgttgaaa agtatagaga atattttgct
    36901 gataaaggga tatttgaaaa taaaatttat gagaatatga aagagatatt agaaatgctc
    36961 tataaaaatg gtaaaatatt attagtagca acttctaagc caacagtttt tgcagaaact
    37021 atacttagat attttgatat agatagatat tttaaatata tagcaggtag caatcttgat
    37081 ggtacaagag tgaataaaaa tgaagttata caatatgtac tagacctatg taatgtaaaa
    37141 gataaagata aagttattat ggttggagat agaaagtatg acataattgg agctaaaaaa
    37201 ataggtatag attctattgg agtattgtat gggtatggtt cttttgaaga gcttagtgaa
    37261 agtgaaccta cttatattgt cgaaaatgta gagtctataa aagatatttt gttataaata
    37321 tcttttatag acaaaaatat tacaaaatgg tgtcgcatat ttaaaaatat gatattatag
    37381 ttaaggaagt catttaaaaa agaggaggat gcacttaaaa tgaaatctat tttaattatg
    37441 ggtggaagcg acttcatagg ttctgcactt gctaagcgct tgattaaatg tgggtatcaa
    37501 attgatatac ttacaaatgg taaaaaggaa atagattata atggttttaa agaacatcta
    37561 atttgtgata gaaaagttcg aaaagatatg gaaaacatta tcacaggaag aaaatatgat
    37621 tacatatatg atatgacagc atatacaaaa gaagatgtaa gtaatctgat tgattttata
    37681 agcatggata acttaaaaaa gtatatagta ttgtcagcag gagcagtata taaagatagt
    37741 ggaagaaata taaaagaaga aaatgagaaa ggtgaaaatg aaaactgggg aaaatatggg
    37801 ttaaataaaa aagaggctga agattttatt ataaatagcc ctataccata tataattatt
    37861 agaccaactt acatatatgg tgaaaataat aatttatata gagagtatta tttctttgaa
    37921 aaaatagaaa aaaatgaaaa gataccagta ccaaaaggaa aacaagttag taatcaattt
    37981 atatatattg gggatttggt aaaggtattg gaaagcataa tgaaaaatcc tcatgtaaga
    38041 gaggcctata atgtgacaaa tccacagtta atttcttggg atgaccttat atatacatgt
    38101 ggagaaatta ttggcaaaga acctataata aaatatgtag atatggaaaa agtagagttt
    38161 agagaaagaa cgtatttccc atttagaaat atagacttta atcttgatat aaataagctt
    38221 attgaacatg ggctttatat accaaatgtg ttactaaaag aaggtcttac agcaacatat
    38281 aaatggtttt cagcaaataa gccaaaaatg catgacagaa aaatgaacaa agttgagtct
    38341 gtaatgcaaa tagtttaaaa aaataaataa gtaggtagat attttgaact caaaaattaa
    38401 gtatttttta aaatttttta tcttaatatt tattttgatt tttatatact cagttgcgat
    38461 agagccgagt ctattaattg tgaaaaaata taatataagt gaaaatggag cattgataaa
    38521 aaatgataca attgagagta aatctgaaag tgtaaaagta gttcaaattt cagatactca
    38581 aataggtagt ttttattcaa ctaagaatct caaaaaagta gctaataaga ttaacacttt
    38641 gaatccagat ataatagtat ttactggtga tttaattgat tactccaata aaaatccaag
    38701 tgtagatgaa attacaacta ttttatcaag tatgaatgct aggttgggaa aattttcagt
    38761 atttggcaat catgattata tgtataaact tcctaggtat tataggcaga ttatgaaaaa
    38821 ttccaacttc aatcttttag taaatgaaaa taaaaaaata aaattaaaag atgataaata
    38881 tattaatata ttaggtgttg atgaaatatt aaatggaaat cctaatataa aatatctgga
    38941 gagtcagata gataataaaa attttaattt attgttagca catgaaccag atttagtaga
    39001 tatgcttagt aaagatacaa tgaatttagt tctatctggg catagtcatg gtggtcaaat
    39061 aagattacct attaagggag cattagttac tcctccatat gggaggaaat atactaaagg
    39121 gttttatgat ataaatggaa atcatttata tgtgagttcg ggtcttggaa gtacaaagtt
    39181 accatttaga ttttttaata taccagagat aatagaattt aatattataa tctaacataa
    39241 aatatttaga aaatataatt ttgcttttta taatatgtag atgtatatac tgtaaaaatg
    39301 gattaaacca gtagattgaa tgaattaata tattaaatta atatataaaa tatttaagtt
    39361 ataattaatg tattagacaa aataaatact attaagaact attaaaatat agattaaaga
    39421 taataacata taataaataa aaaagtaaga ttgtgtagat tatataaaga ttatttataa
    39481 ttaatacaat cttgcttttt tatttaaaaa ttttaattat tatttaaaat tttttatctt
    39541 ctctttatcc catataaaca atttctcaga attatttaaa gcgccaaata catttctttt
    39601 aaaatcaggc atctctttat ttaaattagc aataagattt tttatgtact ctctcctagt
    39661 atgaccattt gcagggcagg gatttgtgat aataggatag ttagaatctt ttgaaatttt
    39721 cttagtcata tactcttcta tgtaaatcat aggtcttata actgttaaat cctgtctatc
    39781 taggtatgtc tttggggaaa aacaatttac tctaccctca taaaacatag acattaaaaa
    39841 tgtctcaatt gcatcatctt tatgatgacc aagagctact ttattacatc caagtttttt
    39901 agcattatcg ttcaaagcac ctcttcttaa attagcacat aatgcacaag gatttttttc
    39961 ttgtcttata tcaaatacaa tttcttttat gtcagtttga aattcataaa aaggaacatt
    40021 tatttcttta cacaatttat ctagtggaga attatcaaca cctccagtat ttaatgttat
    40081 ggctatcaat tcaaagtttt gtggagaaaa tctttgataa cttttaagta tatgaagtag
    40141 agttaaacta tcttttccac cagaaagtcc aaccgcaatt ttatcatttt cttgtatcat
    40201 atcaaaatct tgtatagcct tacgagcttt acttagaagt ttttgtatag acattttatc
    40261 accatccttt aaaaatatta aatttaagat ttatttaaga tttacttata aaatacttaa
    40321 gtcttatact ctaaaattaa cattatagac aaacataaca aaaaggagaa gatttaatta
    40381 tgaaaaaatt taaattaata ggagccatta ttttagtagt atttataagt gtatttatat
    40441 ctaaagatag attacttaag aaagataaag atccaatagt agctgctcaa catgattcag
    40501 aacaagatgt tgaggcaaat tctcaaaata aaaatcactc aaatagtata tcaaatatat
    40561 tattggtaaa caagacaaat ggaattagca aaaactatac tcctgaaaat attactaagg
    40621 taaacatacc ttttgttgaa gaagcaacag aggaagaaaa acaaatggca ggagaacctg
    40681 caaaggcagt tgaagattta gttaagcaag ctaattcaga aggaatacag tttttagggt
    40741 cttcagcata cagatcttat gatactcaac tggatactta tacaagaagg gtaaaatctc
    40801 agggacgaga aaaggcagat gcatatgtag caaagcctgg ttacagtgag caccaaactg
    40861 gactttgtat tgatttaaca aatccagaaa gatggtttgt tggaagtaca aaagaagcta
    40921 aatggttggc agaaaatgct cacaagtttg gatttataat aagatatcct gaaggtaaag
    40981 aagatataac aggtacagca tatgaaccat ggcatattag atatgttggt aaggatgctg
    41041 ccgaggaaat ttattcaaaa ggattaactt tagaagaata tttacaaaat aaataatata
    41101 aatgtgtaaa taatttgtat tatattggaa atttagaaat gaataaattt atagattaca
    41161 ctaactaaaa tatagtataa ttttatatga ataaagctat atacaaagat taagttagga
    41221 tgtgtaaaaa tgaataatac agttcttgtt gtggatgatg aaaaggaaat aagagacctt
    41281 atagagatat atcttttaaa tagtggatat aatgtaatta aagctgaaga tggaaaagag
    41341 gctctagata ttttaaaaaa ggaaaatatt catttaatga tattggatat aatgatgcct
    41401 aaaatggatg ggatagaagt ttgtagaaaa gttagagaaa gtttgaatat accaatactt
    41461 atgttaagtg caaaatcaga agatatggat aaaatacaag ggataatgac tggtgcagat
    41521 gattatttga caaaaccatt taacccactt gaacttactg tgagagtgaa atcactgatt
    41581 agaagagcat attattttag tggtgctaat aatgaagata aagatgtcat aaaggttgga
    41641 gtagtaatta tagataagga aaaacatagt gtaacagtgg ctggaaatga aataattttg
    41701 acatcaagag aatttgatat attatattta ctagcaaata ataaaggcag agtatatagt
    41761 acagaagaaa tatttgaaaa ggtatggaaa gagaaatatt accaatctaa taatactgta
    41821 atggttcata tgagcagaat aagggataaa attgaaaaat atacagatgg agaaaaaata
    41881 attcatactg tatggggagt aggatataaa attgaaaaat aaaagtttat taaatcatat
    41941 gtttcgaccc atattagatc ttataataag ctgtgcttta acttcaatgc ttattttaac
    42001 aagtatgtat ataataaaat tattatattt caatagtgag atatctgtaa ttaatcaact
    42061 tgtatatcag ttaagagatt tcagaagtct tataggagag agtttatttt atccattttt
    42121 atttacaatc acatcattag caatttattc catgatatcg tacaagagaa ataagcaaat
    42181 tgtagatttt ataagaaaga ctaatagaaa aaatcaaaaa ttagaaatca ttaaaagtgc
    42241 tgtaaattta atggatgaag gtaacttaga aaaaagtata gatattgagt atgatttaga
    42301 tatattaaag gataaagaaa aggatgatat tgatgaacta gcacataaaa tcaataatat
    42361 agtccatcaa cttagaaata taactattga agagagacaa gctcagcaga caaaaacaga
    42421 tttaataaca aatgtgtcac atgacttaag aacacctttg acatccataa tgggttattt
    42481 aggagtgata gaagaaggta agtataaaga tgaagttcag atgatgtatt atgttgatat
    42541 tgcatatgaa aaatcgaaaa atttaaatgt tcttataaat gatttgttcg aattgactaa
    42601 aatgcaaaat aatacaataa aactaaacaa aatagaaata aatttagtag agttattaag
    42661 tcaagtagta tctcaatttg aaatttattt taaacaagaa tttatggtta gtagaattaa
    42721 ttttaatgaa gaaaaattaa ttgtgaaagc tgatccaaat aaattggtaa gagcatttga
    42781 aaatcttata actaatgctg tgaaatatgg aaaagatggg cattatgtag atatagtcac
    42841 agagaaaatg gaagatatgg cggttgtaaa agtgataaat tatggagaac caattccagt
    42901 gttagattta ccaaatatat ttgatagatt ttatagagtt gaaaaatcga gaaatagaaa
    42961 tgttggaggt tctggattgg gtcttgcgat tacaaaaaat attgtaaatt tacatgatgg
    43021 agaaattact gtttcaagcg ataaatatca aactgtgttt gaggtacaac tgccaataat
    43081 acaatcagct tagttataaa ataaaagtac gattatgtga tataaatatt ataacaataa
    43141 agtgtttaca aaaataaaaa agtgatatat aatttaagtc agtttaataa gatacttatc
    43201 aagaattagg ggagagagtt agctcagtga tcctatacca accttttatc aggtggtcct
    43261 gctaacaacg atgagatgac ttgaaataat atactcttct tatcaataat agataggaag
    43321 agtttttttt agaattaatg taacaaactt taaagtataa tggaggatta aataatggaa
    43381 aaagtagaaa gttttaagct agaccataca aaagtaaaag ctccttttgt aagaaagtgt
    43441 agtgtacttg atggagtaaa gggagataaa gtaactaaat ttgacttgag atttttacag
    43501 ccaaatgtag aaagttttgg aactgctgct atgcacggtt tagagcatct tttagctact
    43561 tatttaagag atacattaga tggtgtaata gacttatctc caatgggatg tagaacagga
    43621 ttttatttaa tactatgggg agatgtagat gccaaaacag ttaagattgg tttagaagaa
    43681 gcattaaaaa aagttcttga atcagataaa atgccagcag ctacagccat agaatgtgga
    43741 aactacagag acttatcttt atttggggca aaggaatatg caaaagatgt tttagataaa
    43801 ggattttctt taaatatata tggagaataa tttaattgga agagaagata taagcataaa
    43861 tcaaagtgaa gttttaagat atttacaata cagaggtcaa gagatagata aagaccttaa
    43921 atatactatt gatgaatgtg tagctataac taaaaataaa ataaatccta gatatttatc
    43981 taggatttat cctattagaa taaataaaga taatgttgta gaattagagg gaacaaattt
    44041 aacattagag agtaaagatg tatatgaact tttaaaagaa tgtaatgagt gtgtaattat
    44101 ggcagctact atagggatag atatagaaaa agaaataaga cgacactctt attcaaattt
    44161 aacaaaagga ctaataattg attcatgtgg aacaacagca atagaagaag tgtgtgacat
    44221 tgttcaaaat aatttagaaa aggttttttt aaaccaagat aaatttataa ctatgcgata
    44281 tagcccagga tatggagatt taccaataga aaaaaatata gatatattaa acgttttgaa
    44341 tgctcaaaaa caaataggtc taacaattac ttctaatgga attatgatac cgagaaagtc
    44401 agtagtggct attataggta tttctaatac aagaataaat aaatctaaga aagaaaagtc
    44461 gtgtgaaaat tgtaaaaact atagaaactg tacatataga aaaggagcga actcttgtgg
    44521 aaataagaca atatctgaaa aataatatat taatatttga tggtgcaatg ggaacaatgc
    44581 ttcaacaaaa agggttaaag ttaggtgaaa atccagaagt atttggtctt caaaatccag
    44641 ataaacttat agaaatacat acagcatact tagaggcagg ttcgaatgta attcttacaa
    44701 atacttttgg atgtaatgaa ctaaaattag atagtaagta tactgtagaa gaggtgattg
    44761 acaatgctgt tttggtggct agaaaagcta tagaaaatgt agataatact aaaccaagat
    44821 atgtagcttt agatattggt cctataggag aaatgctaga gcctatgggt actttaagtt
    44881 ttgataatgc atatgagata tttaaaagac aagtactaca aggcgttaaa tctggagttg
    44941 atgtaatagt aatagagact atgatggatt tatatgaagc taaagtagct gtattagcag
    45001 caaaagaaaa ctctgattta cctattttct gtactatgac atttgatgaa ggtggaagaa
    45061 gttttactgg atgtatgcca gaatgtatgg tggctactat agaaggtcta ggtgtagatg
    45121 caataggagt taattgttca ttaggaccta aacaactttt accaatagtt gaaaaaatag
    45181 catcaagagc tacagtccct gttatggttc aagcaaatgc aggtcttcca aatatagttg
    45241 atggagaggc tatatatgat gttgatgcaa aagaattttt tgaaggggtt aaaaagttcg
    45301 ttgaagttgg tgctactata ataggtggat gttgtggtac gaatccaagt tttataaaag
    45361 aaatatctga gaatataaat tctgttacaa agggatgtat cgagaaaatt gataaatgtg
    45421 tagtctgttc accatcaaaa tttgtggaag tacaatcgcc tacagttgtt ggagaaagat
    45481 tgaatcccac aggtagaaaa tctctacaag aagcattaaa aaatgaaaat gttgattatg
    45541 ctataaattt aggcttagaa caagtaaatg ctggagcaca aatacttgga gtaaatgttg
    45601 gtcttccaga gattgatgaa aaaaaactaa tgccaaaatt aataagagag attcaagcag
    45661 tagtagatac acctttacaa gttgattctt ccaatgtaga ggccttagag caaggactta
    45721 gatattataa tggtagaaca atagtaaact ctgtaaatgg caaagaggag tctttagaaa
    45781 gtatattacc aatagttaaa aagtatggtt catgtgtggt tggacttaca cttgatgaaa
    45841 aggggatacc taaaaaggct gaagaaagat ttgaaatagc aaaaaggata gtcaatagag
    45901 cagtttcttg tgggataaaa ccaaaagata tatttataga ctgtttatca ttaactgttt
    45961 cagcacaaca agaagaagca atagaaacta taaaagctat aacaatggta aagacactag
    46021 gagtaaaaac tatattaggt gtttcaaata tttcatttgg tcttccaaat aggaaagcac
    46081 ttaatgcttc ttttctaaca ttagctttag gggcagggct tgatttagca attataaatc
    46141 caaatgaata tagtatgatg gaagctatca attcttttaa aatattaaat aacacagata
    46201 aaggatgtat aaactatata aatcaatata gtaatataaa taattcaaaa aaatcggatt
    46261 catctacaaa gattgacaaa gatttgccat tggatatttt agttgaaaga ggattaaaag
    46321 atgaagcaaa aaatgtaaca ctgaatcttt taaaagagaa ggatgaaaat tacatattag
    46381 atgagatttt aattcctgct cttgataaag taggaaaaag atatgatagc ggagatatat
    46441 ttttaccaca attgattcaa tcagcagaaa cggtaaaagt ttcattaaat acaataaagg
    46501 aaactttatt aagtaaaagt agcaataatg tatccaaagg aaaaataata gttgctacag
    46561 taaaaggtga tatccatgat attgggaaaa atatagtaaa aataatgcta gaaaattatg
    46621 gatatgaggt tatagattta ggtaaagatg tccctattga agaagtagtg gaagttgcta
    46681 aaaagagaaa tataaaatta gtaggattaa gtgcacttat gactacgact gttcaaagta
    46741 tgaaagatac aattcaggca ctgagagata atgagataaa tgcaaaggta tttgtaggag
    46801 gtgcagtttt gacagaagaa tacgctgaag aaatgggcgc tgattattat tcaaaggatg
    46861 caaagtctgc tgttgaaata gcaaagttaa atttttagca tataaattaa aataaaggtt
    46921 ttactaaata ggatatttta tatataatat aaatataagt tttagatact tatgtttaaa
    46981 acaataggaa ttaatctata ataatgagat taattaatat aaaaaatatt tatcatcaac
    47041 attaaaaagg gctgggaagt ctggtataac ttccattagc cctttttagt taaaatcata
    47101 tgttaaaaag cccaattacc atttttaaaa atctgtattt gttttccatc ataagtctca
    47161 ccaataatat ccatatcagc agaaccaatc ataaaatcta catgtgttaa actgtcattt
    47221 acaccatgtt catctaattc ttcatctttt aaattgtttc caccttccag acaagtctta
    47281 taagcagaac caattgcaaa gtggcaagaa gcattttcat catatagagt attataaaaa
    47341 attatatccg tattggatat tggtgagttg tatggaacaa gagcaacttc tcctaaataa
    47401 tgagagcctt catccgtttc aataagtttt tctagtgtat ctaatccctt ttcagcagag
    47461 aaatctatta ttttaccatc tttaaatgtt aagaagaaat tgtcaattat gtttccacca
    47521 taaacaagag gttttgtact ataaacagtc ccatttactt taaacttatg tggcattcca
    47581 aaaatctctt cagttggaat attcggatta aaacttattc cattagggtc tttggatgct
    47641 ccacttagcc aaacatgacc ctttggcaag tctagagtca aatcagtttt tgaggattta
    47701 tattttaaag ttttaaagtt atttttattt agaaagtcca ttttagattt aagattttca
    47761 ttatgttctt cccatgcttt tatagggtcc tgaccatcta ctcttgaaca tttaaatata
    47821 gcatcccaca gtttttctat tgctttatct tctgataaat ttggaaacac tttttttgcc
    47881 caatcttttg ttggcacaga gactatactc catttacatt tatcagttag agtgtaagaa
    47941 cgccattctt ttaaagcttt tccagatgcc ttctgaaaat ttgcaactct ttcagggtca
    48001 acatctttta ataaatctgg attttgggca tagactgata aaaatgcccc accttctttt
    48061 gctatatcta catattgttc tgctgtccat ttggggaact catgaaatgc ttcatctgga
    48121 gcatacaagt atttaataag agtactttct tcatcagacc actctatata aacattttta
    48181 gcaccacatt tatatgcttc tttaagtgca tttcttgcaa aatcagcaca ttctattggt
    48241 gactttataa gtagaatttg acctggctgt atgttaactc caacttttat tgctaattca
    48301 gcgtattttt ctaaatttct atttaaattc atatttatcc tcctatattt ttaatattga
    48361 ataacttata aagtattata acattactta tgtttttaat attgaacaac ttataaaata
    48421 ttataacttt acttagattt tgggtcatat tcaaaatatt tatactttat agattgagaa
    48481 ataaaaaaaa tattttaatt aaaacagatt atgaggataa caaacttatt taagcagtat
    48541 gttttttgtt gataataata ttaaagtaga aataaaaata cttttttagt gaaaagctat
    48601 acaagaatac cgaaaaaaac ttataatata ataatgagat agatatttat aaatgaaaaa
    48661 aatttttctg gcaaagctta attaaaaagg taatagctag tgttgttatt taaattagca
    48721 tagaaaatca aataaaaaaa ttatgctaat gtatagacat tgataaggta gaatttaagc
    48781 cttttgaatg ctatattaaa ctagagtaga tattcttatg aatttcaaaa tagggcagga
    48841 ggaaaaagga aataaaacta taaaaaatat aaagtattta tataaattta tagagtttat
    48901 acgtcagaag atttcagtta ggagggcata aatggagctt tatgaagcca ttttttatag
    48961 aaaatctgta agaaattttt ccagtaagaa gataaaaaag ccattgatgg aagaaataaa
    49021 aagaagttgt aaaaatttag aatacttaaa tgaagattta aatattaaag cacatgttgt
    49081 tgatagaggt catataatac attttttgat gggtaaggaa tgtaaagtaa aggcacctca
    49141 ttatatagta gttacatcag acaaaggtga ggactattta cagaatatag gatttgctac
    49201 agaaggtgta gttttgcgcc ttacaacatt gggaatagct acttgctggt tggaaggaaa
    49261 cttaaagaga gaagatatct tggaatttgt agatattggg aataagaatt ttgaagaaga
    49321 tgaggaagat gatttagatt atctagctgg agtaaaaaca gaagaagatg atgaaaacaa
    49381 aatagaaaat ccttatataa taatagcatt tggatatcca gcaaaaaatg aagagttgtt
    49441 taggacgaga tatgctagac ctgatagaaa gccagtaaaa gatatgataa ataagataaa
    49501 taggaataga aagtggaaag atatcttaga attgacaaga agagctccat ctgtaaaaaa
    49561 ttctcaaccg tggtatttcc ataaggatga acgaggtctt catttatttg aaaagagacc
    49621 taagaaacac tgtgaagata tgaataaggt tagcttaggt gtagccttaa gacattttga
    49681 tatagcttgt ataaaaaata aaatagatgt ttcatatgaa aaacttccta taaggaataa
    49741 aataggaaaa tcatatttta taactgtagt cgagcatgtg aaaccagaag aagaaaccca
    49801 agaagaaaat gtaactctag aaaaagaaga atcacaagat gaataaaatt ttggaagggg
    49861 atattggata tgtataagtg tggaaaatgt aatcttagag gctgttctaa tggaaatatg
    49921 gataaattaa tgaagatatg tccaactaga gaaattgaac tacaacaaaa agcaaagcaa
    49981 ctttatgaag aagaagagaa tagaaaaata gcttataatg ctggattagt tgaagcagag
    50041 gggtattgtc aatatactag actaaaagag attatagagt ttgctaaaaa gtgtggttat
    50101 aaaaagattg gcatagcatt ttgtattgga ctaaaaagag aaatgcaaac agcacaaaaa
    50161 atatttgaac atcatgggtt tgaagtattt tctgttatat gtaaaaatgg ggcaatgcct
    50221 aagagtacaa ttggactaga agcatctcaa acaatatcaa agtgttcaga tgaggtaatg
    50281 tgcaatccaa taggacaagc tttattttta aatgaagcta aggttgacct taatgtaatt
    50341 cttggattat gtgttggtca cgatacttta gccatgaaat atatggaagc tcctataaca
    50401 actttagttg tcaaagatag agtaactggg cataatccag ttgctgcaat atataatgca
    50461 gaatcatatt ttgagaaaaa attgtttgat aaagatgaag aataattcaa agacttattg
    50521 acataaaaag caattagtgg tttaattata aaaaagataa aactttaaat tatcctatag
    50581 atacataaag ttatataaat aaagaaaaat attacagcta aattccttaa agtagtccag
    50641 agagactaaa gggttataga taatgttagt atataacctc tagtaaatct tctagaggtt
    50701 tttgatgagt ataaaataaa aatttaaata taataaagtc ttttaaacta gtccagagag
    50761 actgggaaag atatctcata gctaatgaat ggagttatgt atagataaaa tccttatgcc
    50821 tcttttggtg tgaggatttt tttatgatta tttaggcttt attaaaaagg agatgtaaaa
    50881 atgataaatg gtaaagaaaa attgatagtt gcaattgata ctgacgaatt tgataaagct
    50941 aaagaactaa tagacagatt agaagatagt gtagacatat ttaaggtagg tctagagcaa
    51001 tacgttgcca caaaaggtaa aacaatagat tatcttaaag aaaaagggaa aaaaatattt
    51061 ttagacctta aattccatga tataccaaat actatgaaaa gtgcagtgag agctgcagtg
    51121 agagataatg tttggttaat gacaatacat gtatcagata tggaaggaat gagacaatgt
    51181 gcactaattg ctagggaaga agcagaaaga ttaaatatag aaaagccatt agtagttgga
    51241 gtaacagttt taacttcatt aagtaaccaa gatttacagg acataggatg taatatgaca
    51301 acagaagagt tagctataaa gagagctaaa ctggcaaaag aatcaggcat agatggggtt
    51361 gtttgttcag cgcaagaagt agataaaata gtagaagcat gtggagctga ttttataaca
    51421 gtttgcccag gaataagacc taaatcagca gaggttggag accaaaaaag agttgtaact
    51481 ccatcagatg caataaaaaa aggtgctcat tatttagttg ttggaagacc tataacaaaa
    51541 gcagaaaatc caagagaatc agctattaat attgtaagag aaatagaaaa tgcttagata
    51601 aaatatttgg gaggtagctt atatgaaggg gaaattaatt ttagaagatg gaacagtatt
    51661 tgaaggaaaa gcatttggat atctaaagga agctattgga gaagttgtat ttaatacttc
    51721 aatggctggg tatggagaaa tattgacaga cgcaacttat tatggacaaa tagttactat
    51781 gacttatcca cttataggaa actatggaat aaatttagaa gatgttgaat caaataaggt
    51841 gcaagtaaaa ggacttattg taagagaaaa aagtgataac ccaaataatt ttagatgtga
    51901 aatggatata gacacatatc taaagcaaaa taaggtaata ggacttgaag gaatagatac
    51961 aagagcatta actaaaatta ttagaaacaa tggaactaag agaggaatta taactttaga
    52021 aaatgtatca ttacaagatg ttcagtctaa attagaagag cattgtaaca aagattcagt
    52081 aaaaaatgtt acaagaaaag aaattgaaaa tataaaaggt actggagcta aagttgtagt
    52141 tatagacttt ggtgttaaaa atagtgtact agagtctctt aaagcttgta actgtgatat
    52201 aactatagca ccagcaatga ctacagtaga agaaatttta aatataaatc ctgacttaat
    52261 cttattatct aatggaccag gaaatccaga ggatataaat gatatagttg aaaatataaa
    52321 aaaattaata ggcaaaaagc cattgacagg aataggttta ggacatcaaa ttttagcatt
    52381 ggcattagga ggaaagactt caaagcttgt atttggacat agaggaggta atcacccagt
    52441 taaatgttta gaaactggaa aggtatttat aacttctcaa aatcatggat ataatgtttc
    52501 agaaatgcca gaaaatacag aggtaactca cataaattta aatgacaata ctatagaagg
    52561 aatgagacat aaggaattac caatatatag tgttcagtac attccagaat acacaacaaa
    52621 agaagattta gattgtatat ttaataaatt cttagcatta atatagattt agtattattt
    52681 acagatagta aaaaccattt agataaggag aaaaatttat gcctaaatta gatagtataa
    52741 agaaaacttt agtattaggg tcaggaccaa taataatagg tcaagctgca gagtttgact
    52801 attcaggaac acaagcttgc cagtctttga aagaggaagg gattgaagtt gtattaataa
    52861 atagtaatcc agccactata atgactgata aagaagtggc agataaaata tatatagaac
    52921 cattaacaat agaatttata gaaaaaataa tagaaaaaga aagaccagac agtttacttg
    52981 caggtatggg tggacaaaca gggctaaacc tagcagttga actttatgaa aaaggcatac
    53041 tagataaata tggagtaaag ataataggta cttcagtaga atctatcaaa aaaggtgaag
    53101 atagagatat atttagagaa gtaatgaaag aaataaatca gcctgtaata gttagtgata
    53161 tagttacaga tttacaagca ggtcttgatt atgcacttac aattggttat ccagtagttg
    53221 taagaccagc atacacacta ggtggaactg gtggtggtat agctgataat gaggaagaat
    53281 taagagaaat actttcacat ggtctacaat taagtcctgt tggacaagta ttaattgaaa
    53341 agagtataaa aggttggaaa gaaatagaat atgaggtaat aagagattca aagggaaatt
    53401 gtatagttgt atgtaacatg gaaaatatag acccagtcgg agttcataca ggagacagta
    53461 tagttgttgc tccaactcaa actttaagta acaaagaatg tgcaatgctt aaaaaagcat
    53521 ctttagatat attaaatgca gtagaagttc aaggtggttg taacgttcaa tttgcactta
    53581 atccacatag ttttgaatat gcagttatag agataaatcc aagagttagt agatcatcag
    53641 ctttagcatc aaaagctaca ggatatccaa tagctaaagt ttcatcaaaa atagctttag
    53701 gttatacttt agatgagata gaaaatgcag taactaaaaa aacttatgct tgttttgaac
    53761 caactataga ttatgtggtg gcaaaaatac caaaatggcc ttttgataaa tttaaaaaag
    53821 caaatagaaa attaggaact aagatgatgg caactggtga aattatgagt ataggaagta
    53881 actttgaggc tgctattctt aaagggataa gatcacttga aacaggaaaa tattcattag
    53941 ttcacacacc atctgaggag agaagcattg aggaattaaa gaaaagagtt gtagtacctg
    54001 atgatgagag actatttgac ttagctgaaa tgataagaag aggctataaa gtagagatga
    54061 tagaacaaat tacaggtata gataaatggt ttataaataa atttaaatgg atagtagagc
    54121 aagaagaaaa attaaaagtt atgaaaattg aagatttaac taaagactat cttcttgaat
    54181 taaagaaaaa gggattctct gataaaggta tatctgactt aatgaagata agtccagaaa
    54241 aattatatga attgagaagt ctatacaata tcaaaccagc atataaaatg gttgatactt
    54301 gtgcaggtga gatagatgct atatctcctt actattattc aacttatgag caatatgatg
    54361 aggtagttgt tagtgataag aaaaaggtga tagtattagg ttctggtcca ataagaatag
    54421 gtcaaggtat agaatttgac tattgttcag ttcattgtgt aaaatcttta agaaaaatgg
    54481 acatagaaac tataatcata aacaataacc ctgaaactgt aagtacagac tttgatacat
    54541 cagataagct gtattttgaa ccattaacag aagaagaagt tttaagtata atagaaaaag
    54601 aaaagccaga aggtgtaata ctacagtttg gtgggcaaac agctataaaa ttggctaagt
    54661 ttttacatga aaaaaatata cctatactgg gaacagattt tgatgatata gatgcagcag
    54721 aagatagaga aaaatttgat gatttactag agagactaga tataaataga ccaaaaggaa
    54781 agggtgtatg gtcacttaac gaaggtattg aagttgctaa tgaattaggt tatccagtat
    54841 tagttagacc atcttatgta cttggtggtc aaggtatgga aataacttat aacgaagaaa
    54901 aattatctca atatctaca
//
LOCUS       DACTGW010000003        54073 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.003, whole genome shotgun sequence.
ACCESSION   DACTGW010000003 DACTGW010000000
VERSION     DACTGW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.003"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..433)
                     /locus_tag="I9573_000134"
     CDS             complement(<1..433)
                     /locus_tag="I9573_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT4794780.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLVFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLELYSKSSEIKAFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFL"
     gene            complement(430..1083)
                     /locus_tag="I9573_000135"
     CDS             complement(430..1083)
                     /locus_tag="I9573_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4794781.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYNDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            complement(1181..2557)
                     /locus_tag="I9573_000136"
     CDS             complement(1181..2557)
                     /locus_tag="I9573_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4794782.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKIFQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSSKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFIIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2557..3213)
                     /locus_tag="I9573_000137"
     CDS             complement(2557..3213)
                     /locus_tag="I9573_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4794783.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3223..4500)
                     /locus_tag="I9573_000138"
     CDS             complement(3223..4500)
                     /locus_tag="I9573_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4794784.1"
                     /translation="MIKNAIAYITRKRNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIGENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNKSENNKIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKHIIKIMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMLNITTLGQ
                     SYLILISIIVLSVVFASSLILIKKPKEILSKIS"
     gene            complement(4519..5409)
                     /locus_tag="I9573_000139"
     CDS             complement(4519..5409)
                     /locus_tag="I9573_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAT4794785.1"
                     /translation="MDRIKNIINGSTKKDKYKIRDRFRHLFQSVWFLITNSYFEGFKT
                     GKIYGGNLKKICVPGMNCYSCPGAKGSCPIGSLQAVIGNSKYKISYYIVGILFFIGAL
                     VGRFVCGWLCPFGLIQDLLHKIPFFKKVETFKFDKHLRKLKYIILLVFVIILPLFLVD
                     ILGQGSPYFCKLICPIGMLEGGLPLVLLNKSMRNAIGFLYYWKGIILIVTLLLSIIIY
                     RPFCKYICPLGAIYSIFNPISIFRYRLDKDKCINCGKCKKVCQMNIDPVENCNHLECI
                     RCGRCKNACPVDAISYGVRK"
     gene            complement(5576..6733)
                     /locus_tag="I9573_000140"
     CDS             complement(5576..6733)
                     /locus_tag="I9573_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAT4794786.1"
                     /translation="MKKIVSRGACLLLTLCLSLSTVGCKKSNESNKIKEGQSISSEEA
                     GMSTNEKDKNETFKPSDYTLKTKKEYVYEYLGLKFKLSDKFKKYMDDKKIAMLDDQSP
                     IDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIGIFEKNTSEEKISK
                     ITKCDTHTKIGVSSDGKYDCYLSTNSGAESNLLDELKRTEIQIIDKKERPKNGFVLSE
                     KTDLENTEAFNKESVKDLRKLSTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEI
                     PDLVEVQNEMKSKGVNIVGVVTDTVDDNGENKEAIEKSKLIHEKTKASYPFLMPDKTN
                     FNGRLNGIQAMPETFFVDSNGNIVGDTYSGAKSSKEWKQVIEKELEKIKNK"
     gene            7067..7417
                     /gene="mobC"
                     /locus_tag="I9573_000141"
     CDS             7067..7417
                     /gene="mobC"
                     /locus_tag="I9573_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAT4794787.1"
                     /translation="MANRIRNERLEIKLTEEEKALFEEKRKLARCRNMSHFIRKCVLE
                     KEIYQVDLEPFRDLQGLLSNATSNINQIAKRVNSTGIIYKDDINDMKKQIEYFSKELW
                     QIHSLLLNRTSGGD"
     gene            7423..8754
                     /locus_tag="I9573_000142"
     CDS             7423..8754
                     /locus_tag="I9573_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAT4794788.1"
                     /translation="MAITKIHPIKSTLNLAISYIVNGKKTDEQILVSTHKCHQETAHT
                     QFLRTRNDVGTNGTVLARHLIQSFLPGEASPKMAHQIGLELCKKILKDEYEFVLSTHI
                     DKGHIHNHIIFNNVNMVTGKCYQSNKKSYHKIRYQSDKLCKENNLSVIDEFYESYKRK
                     YKTNGKSWYENEQSKRGTSWKSRLQFDIDRLIKQSKYWEEFLKKMAELGYEIKYGKHI
                     AFKPKDKTRFTRAKTIGEDYTEERLKERITENQSITTPPVKRRIGNIINMNANAKVKE
                     SKGYEYWATKHNLNTMAESVIFIREHGINSIKQLDKYIQKSAEERQDLQDKMKEIDKD
                     MQLLSDTMEQVHTVKKYRAYYKEYKANPSDKAFFEEHKAEITHYEIALSKLKKSYSKL
                     PNSKDILDKLNKLQEKKNTLIQEYSSTKSTMDELYQIRKNYGIYMGKEMER"
     gene            complement(8790..9596)
                     /locus_tag="I9573_000143"
     CDS             complement(8790..9596)
                     /locus_tag="I9573_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT4794789.1"
                     /translation="MEISKKELRKISRKFRILASNVMNSYYGEQNANLEEMINYIEQT
                     LLIKEYLHSLEYDVDGLDDLLNTISNSYGRKVLNLGNDSNKRTYLLYRAFSFITGNNL
                     PTYTFGWYYAGSNKYQDMAKAFGDRMVYPFVLEVENYMKDISTDMGYDENNALYNINV
                     NSSGVQVNIAEHGSTINADQENKFNTEEVIKAINNVEDLIKGMEDDNSKSILNQNLDN
                     IKNEIKQKHPKKEVLTTCLKSMHFITTSVALIPDLALGIKMLASLIGISI"
     gene            complement(9586..10047)
                     /locus_tag="I9573_000144"
     CDS             complement(9586..10047)
                     /locus_tag="I9573_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAT4794790.1"
                     /translation="MANDIKFEISVPADEEGYVQLKCPSCSEKFMLTVEDIEAESTID
                     IWCPNCGLTHENYLDDDIYELAERIVENQVAEMLNDFSKNLEKSFRNSKSIKFKRGKE
                     IKKEAELPIGRKTGDYEEKKYLCCGKTARISSIKKLEGSYCPFCGEMIDGD"
     gene            complement(10075..11142)
                     /locus_tag="I9573_000145"
     CDS             complement(10075..11142)
                     /locus_tag="I9573_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HAT4794791.1"
                     /translation="MSGKLYIAAAGSGKTRKIIEDSLNTHKKILILTYTITNEQQIIS
                     RIKNKVGVIPNNITVSTWFSFLLKDGIRPYQGSRFKERIEGIIFVNGQNNTYVKKTSN
                     KYFAKDNRVYTDKLSECVVELNDKCNGKVFERISGMYDCIYVDEVQDMVGYDLEILKC
                     LIASRIEVIMVGDPRQCTYSTHSSKKYKKYSCGKIDEFLKNECRKLDVRIDETTLNCT
                     YRNNDIICKFANSLYPERTSTNFVRRAEDESDGVMIIDKADVEKYLQKFNAMQLRENK
                     RTSVNNEYPYYNFGESKGLEFQRVLIYPTQPMWDWIINNNTSLKEQSKARLYVAVTRA
                     ENSVVIVRKDARPCSLPVVKL"
     gene            complement(11139..12761)
                     /locus_tag="I9573_000146"
     CDS             complement(11139..12761)
                     /locus_tag="I9573_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4794792.1"
                     /translation="MSIRKLKIKNFKCFSDWFIVDFENGINILVGNNGTGKSTILEAI
                     NLVLTGTYHGKNIRNELTQYLFNKNIVENYIREVNEGVNVEPPKIIIEAYFDAEMPSF
                     LGDGNSEKTKDTPGICLSISFDEKYSNEYKMLCKNKITSLPIEYYEVSWMSFARENIT
                     ARSIPIKSAMIDTTSYTPTSGSDIYISRIVKNLLDDNEIINISQAHRLMKEQFSRDEN
                     IANINRKLTETSNNELTISVDFGTKTSWESSLVTQLNEIPYNNIGKGTQCIVKTQLAL
                     SDHKIENKQIVLLEEPECHLSYSNLNILLDKVNESVKEKQIIVTTHSSFVSNKLGLEN
                     LILLNRGKTLRLGDLSNETNLFFKRIAGYDTLRLLLSNKAILVEGDSDELIVQRAYMD
                     SHNGKLPINDGIDIISVGISFLRFLEIAKLLELRVSVITDNDGNIDALNRKYSDYLGD
                     NCCENINIYFDDSTHEYNGSLENYNYNTLEPCIYRSNSMSILNEILNKSYEDPDDLLK
                     YMKGHKTEVALKIFESDKAIEFPQYIKEGILI"
     gene            complement(12964..13194)
                     /locus_tag="I9573_000147"
     CDS             complement(12964..13194)
                     /locus_tag="I9573_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4794793.1"
                     /translation="MRVFSYKRLFKKLIDLDMTNNELMEKAKVSKSTFYKMKNGQNVT
                     TDVLLRICNALDCDIEDIMECVIIEDVRRMGV"
     gene            complement(13334..13993)
                     /locus_tag="I9573_000148"
     CDS             complement(13334..13993)
                     /locus_tag="I9573_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAT4794794.1"
                     /translation="MDYKTMRDRVEDMLSDNHKDFVKAIISMEKGINDESALDKLYDA
                     YMDNDSLNLLHEEFDYMIEDLRGQGQIKDLLYVREEKDNLINIVGNIVGEVDVIEREN
                     KNGETFKVANFSVVSKDDEGNKLYHNCSVYGEKSDIPKDFKQGDFVKLFGQIRTSIDD
                     NGKEHSNIRILSSKLLKAKEQMKGQEEKKESILGAIKKYQAEDKEKPKEKKEASKEAE
                     R"
     gene            complement(14036..22765)
                     /locus_tag="I9573_000149"
     CDS             complement(14036..22765)
                     /locus_tag="I9573_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAT4794795.1"
                     /translation="MRINDFHNILELIKQDVLYNEAEYLKLLKVVGNNQRYDFRSQLS
                     IYDKNPEATACAKFDYWRERFNRTVMRGQKGIPILEDYGTYKKVDYIFDISQTVSKNR
                     DVNEVNLWRFDKEAHQDVLKEMIKSEGYEESESTLENIFSLSRLYGDEKIDTLMNELR
                     VADEDRISFTKFVRDSVSYAVASRFKLDYPMDYELLRENFQRFDSISLMSLGESVSDI
                     SGKIIDETIQKSKELELQKEVLIGKEAGYNKIKEELEEVEENVFRRDDQERNENERVL
                     RNGEYGRDNRKNQGEYAKQLGGTDGLYKEVSESDLRSDEAELPFTERGAEPLRDAFGS
                     IQGEEADRTPDGYSETGSRVYESREAKVDSSLEDRGREQSAVWGDDFSSQRNDHQGSS
                     GDLKDNTDKGIIEAEKASFSLPENSYGQISLTIPLTEKDIDTVLINGGNHDGGRLPVI
                     AEFSKGKSIEELGEYLKDTFKGGNGFYIDEREVSSWYSDKGIHLAYGTSAREDNTQIL
                     SWSDAASRINELLDRGEFATNVELLEAQDYERDRISESLWYLTHDLSEEGIEQGYFNS
                     FERGGGFPQETKRLSEALKNPEYLKETIKEYSRFLEGYKESRNVLRYHYHKVDSLYQK
                     LQELELQRKEYSTNLTELPKVKLFITEDEVLESLSRGSGVDRGKERITKFFKENHTLQ
                     EKANFLKDEYGIGGHSHVVSGAMGSDEWHDAKGFKLQKNDCNDVFLTWTSVAKHIDEL
                     LSKKLYFEEKETESTAEIEEVKEPQYYSKNDPGNLMPDEILERVPEIYAQEDVDLADK
                     QVHAAYIIPFRSNWTWYMTEYDRESGDAFGLVLGIEPEWGYFNLEELKELNAQRLILE
                     DFPKTFRELKDIELIKQMDEQELQFVFNGELSFEDKVELEAPEETEEATKRGTVQATL
                     FDYLKEREEVELNEKEDSLLEEFAVKAGDTVYFNHEEYKVREISKNEITGRYDLWLDP
                     VRSGNHQIPIVDFTDNEDLLNKISLERPNFIVGDEVKYKDKNHTITRFDDMRKNLNTV
                     TVKDNTEYFGGMITSFDVIPYRLESDLERVFENLTYKQPEQTTEETQIRKAEAHNFKI
                     TEETLPEKLSPSERLNNNLEAISMLNRVESGQRELDSTAQEVLARYVGWGGLSEVFDE
                     SKEGQWKEARAFLNENLSHAEYEAARESTLTSFYTPKTVIDGIYKTLSDMGFKQGNIL
                     EPSMGIGNFIGNIPDEMNKSKFYGIELDSVSGRIGKLLYPESEVQIKGLEETSFSNNF
                     FDAVIGNIPFGEYKVNDREYNKNNFLIHDYFFAKSIDKVRNGGIIAFITSSGTMDKKD
                     ESVRRYLAARAEFLGAIRLPNDTFKGIAGTEVTSDIIFLKKRDSIRERDEDWIHLAED
                     ENGLTYNKYFVDHPEQVLGTMREVSGRFGNTLACLPKENADLKELLTKASEEISKGST
                     YEEIELLDDEITSIPATDDVKNFSYTVIDDEVYYRENSLFIKKEITDKNKEKIKDYLE
                     LNTALKDVIYKQKEDYSDDEVKKAQEKLNEVYDNFSKKHGFINNLSNTRALKEDSNFP
                     LVSSIEILDEEENVKAKGDIFSKRTITKAKVIDHVDTSLEALVLSVSEKGYVDFEYME
                     SLTGKDRPTLIEELRGEIYLNIREEQNFYRPLSFNLEDGDLPFACANGSNSYKYGYVT
                     KDEYLSGNIRDKIAIVDSYLAKLRQTERELPHLGFVENGKEKELISYEMNRLEYQKAE
                     LTKVLPKELEASEINVRLGATWIPIKDIEKFIFETLKTPGYARWDIKVKFSNITSEWN
                     VEGKSRDKGNDLAEMTFGTSRVNAYKLIEDALNLKETKVFDQIINPDGSKTSVLNKKE
                     TMLAGQKQELLKEEFKNWIFNDQERRNRLVKLYNERFNSIRNREYDGSNLSFEGMNTE
                     IDLRPHQRNAIARSLYGGNTLLAHVVGSGKTFEMVASAMESKRLGMCSKSLFVVPNHL
                     TGQIGREFMQLYPSANIMVADKKDFEPKNRKRFIGKIATGEYDAVVIGHTQFEKIPMS
                     KEYQEKHIQDQINEIVNYVEEYKHDRNQNFTVKQLEKTKKKLETRLEKLNDDFKKDDV
                     ITFEELGVDMLFVDEAHNYKNLYLYTKMRNVAGIGQSEAFKSSDMFMKCRYMDEMTGG
                     KGIVFATGTPVSNSMTELYTMQRYLQYESLKKNHLEHFDSWASTFGETQSSFELSPEG
                     TGYRVKTRFSKFYNLPELMSMFKEVADIQTADMLNLPTPEANYEVIKTEPSEEQKEIL
                     KSLSERADDVRNRVVEPDVDNMLKITNDGKKLALDQRLINPLLPDNPDSKVNVCVKNV
                     FAIWDKTKENRSTQLLFSDMSTPKGDGEFNIYDDIREKLVAMGIPKEEIAFIHEANSD
                     KQKDELFAKVRKGEVRILMGSTQKMGAGTNVQNKLIALHDLDVPWRPADLEQRAGRIV
                     RQGNENEKVNIYRYVTENTFDAYLWQTIENKQKFISQIMTSKTPVRVAEDVDESSLNY
                     AEIKALATGDPKIKEKMDLDNEVTKLKMLEANYKSNRYRLEDKVAKNYPEEIARTEKL
                     IEAVKKDISNVEPKAEGEEKFTSITIAGEKITDKKLAGERLLEAISKVKINESKVIGK
                     YRNMDLEVSYNFFTNSHNFSLNGVAKHLGELGTSADGNITRLDNALEKMPEKLNMLEE
                     KLISTKEQLENAKEELKKPFEKADELKTKVLRLAELNKLLDMGEVEEKKNDNPLVEDV
                     KRAIIDFCNREYEENHSYDEFHTLYPDLKHIGIAYTNTPDERHGIQYELNLEEKTWTQ
                     YIDDTPIKTESFDYENKGENEALRNMKNEIELSTFSDLVYVDSEDLRVATGLDIDDEG
                     NFYDPFAKDLDNDGIPDRYDSDFKDSDYFESTYDVEDNLHSKEETTQKSDDKPSILGQ
                     IRAYQEESKTEEKQKIKEQEFLR"
     gene            complement(22848..23651)
                     /locus_tag="I9573_000150"
     CDS             complement(22848..23651)
                     /locus_tag="I9573_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAT4794796.1"
                     /translation="MSKINVEGSEISIIAIDNRDYISLTDMVRNIENGSALIEKWLRN
                     KNTIEFLGIWEEMYNTNFNSPEFEGIKNEAGFNRFILSVKQWVEKTNSIGIVARAGRY
                     GGTYAHKDIAFEFASWVSPYFKLYLIKEFERLKEEEQKKLGWDIKRNLAKINYRIHTD
                     AIKNKLVPDKLSKEKINFIYASEADILNVSLFGITAKEWRDENPDLKGNIRDYADISQ
                     LVCLSNLENLNAVFINEGMSQSERLEKLNAIAIEQMKILSESESIKKLK"
     gene            complement(23736..25442)
                     /gene="topB"
                     /locus_tag="I9573_000151"
     CDS             complement(23736..25442)
                     /gene="topB"
                     /locus_tag="I9573_000151"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAT4794797.1"
                     /translation="MKLVIAEKPSVAISIAKVIGANKKKDGYYEGNGYRVSWCVGHLI
                     QMANPDAYDEKYAKWNMADLPIIPSDYKYEVAKATKKQFNILKKLMNDKEVDTVINAC
                     DAGREGESIFRLVYNQVNCKKKMKRLWISSMEDSAIKEGFDNLTDGKDYDNLFESAQA
                     RAIADWLVGMNISRLYSCLYQQNYSVGRVQTPTLAMIVKRDDEIANYQKEKYYTVELS
                     MNGFTLSTDRIDDEVAAEQLISLVGDKIEITDVIQKEKITKPDLPFDLTTLQRECNKY
                     FGYSAKQTLDYTQSLYEKKLITYPRTDSRCLTEDMIVSTVNNILGKNDFDTERIKVVF
                     NSKKVTDHHAIIPTISSVKEDVSELPLSEAKVYFLISDKFHASVGYPLIENTTKIVAS
                     FDGFEFTSSGKVIKDEGFSKYLKEYKSKKSEDAVLPDVSVGDVLSVENKEVKEKFTQP
                     PKHFTEDTLLKSMEIAGNDALEKGVEVERKGLGTPATRAGIIENLIFKRFVERDKKNL
                     IATHKGISLVTIVADTFKSAETTAKWEMELSDIAQGKSSKKEFLDAIENEIKEAVLTY
                     RK"
     gene            complement(25537..27078)
                     /locus_tag="I9573_000152"
     CDS             complement(25537..27078)
                     /locus_tag="I9573_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012858678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAT4794798.1"
                     /translation="MKTSLKKNNKFWTVALAVIVSISCILGLWTVVYAQEQKSEAPTK
                     NEAVQTEVEVNVRYIFEDEKVFKEEKIKAEKGQLIDSGDLPMLPDDMKFIDEFLFYEV
                     KGDGKDEIIRKVAKMEVKDKETQTEEEKPKQDECTQTEDKGTQTELSKDDISKIEKEA
                     KELQDKLDKLNCELKDKDKLSDKQKNKIKDLEDEIDSLKEKMKKDKGNKDLSEDMKKE
                     IDKLTEKVKELEKKATETNKAPVTSQSVTPISPISGIKTSSGISPQTPQTSVSSTGKG
                     SSDSVSSGNTTKDTGKGKTEVKEKEKEVRYPNKLTAKAPANNSQDSSMGSASKSVNTN
                     KGVASSPSKARASVTENKDNANKEYPIHHGDSSDNKETDQYSADARQFITFQTKNGKT
                     FHLIINHDEDSENVMLLTEVSEDDLLNMVEKKEAPKQEVVKEEPVKEEVKPEKKDEKS
                     NLGTYIILLLVVGGALGAGYYFKVVKKKEDKELEALEEEDDDFFSESESEEEINDADE
                     VEDEE"
     gene            complement(27053..27292)
                     /locus_tag="I9573_000153"
     CDS             complement(27053..27292)
                     /locus_tag="I9573_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT4794799.1"
                     /translation="MKKELTTVKNRIKKLKDKKALIDEELEPLFIREEELENEEIIAI
                     CRKNNITISDLIAKVNRQKAEMKKEKTNENQFEKE"
     gene            complement(27304..29436)
                     /locus_tag="I9573_000154"
     CDS             complement(27304..29436)
                     /locus_tag="I9573_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAT4794800.1"
                     /translation="MGKKLKKDFKERHKASLEREMFHSKTDTIPEESKLKHNDDYRGK
                     IVHEKERFQDKVHEKTSKVSADNEKSYGTSKRNEKYRTSDKGDVNSAIETGRSDYITE
                     VKDGKIYDPLGKDLDNDGIIDRYDNDFRDSNYFESTYDVEDNLSHKEKSLENSFKNHK
                     TQKSRYKRKNYSESLYTRKKDDAPKGNKADDKKAGKDAVSGKVYSSLSKDQKKKLKKD
                     MVKVSALSGLAKGSETVRDYLSHGSDENQGVEAGEKTADTSSKLIHGIKKYSDKKRAK
                     RGYDLTNKDYKIRKRKSKLEFRDAKKELKKTDEYKRASAYKRFQKKNQVKAAISRENK
                     SRLRDRIKEGLIGTLKSSKDMIIRKAKGLMLIFIGIIILGTFVINFAGTGMTGFMNST
                     SSVLTTSYLSKPNVLSEINQKFSDMEGELQSEVDYVKENYPGYDEYILNNTEYIGHNV
                     HELLSYITSRCGEVKSVSEVESILKELFESMYDLEYREEIEIRYRTVTETYTDEDGNE
                     YTESHEEPYEYKKLIVTLHKKEMDSIIRKVFANYPDNLKHYEALFLAQGNMREAFGNS
                     DLISANGGIGGGKEYEASTEVQKKIVNAAYITPSPGAGWCAMWVSQVYQNAGLGYIGG
                     NACDMYRNYTFTSDRSKLKVGMLVAVESSSSGSSAGLTYGHVGIYIGDGKVIDNIGRI
                     RVTTLDDWIATFCKHHPVGFGFPPNVKK"
     gene            complement(29440..31869)
                     /locus_tag="I9573_000155"
     CDS             complement(29440..31869)
                     /locus_tag="I9573_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAT4794801.1"
                     /translation="MNKKRKIQQIKLMQNEKALRSQKRDLKKSNCKSKKDKGGILDLI
                     FKKEPKRYTVEDTIPYLRLLKSGICQLDEKHFSKSIAFQDINYQLALDEDRDLIFNQF
                     ANFLNSFDPSISIEFSYINQLGRNEEMKSAIQIPDKKDGFDDIRFEFREMLKSQIVKG
                     NNGLKKSKYVTFTVEADNLEQATSKLERLEIDILSSLKSMGVRAESLNGEERLKILHD
                     VLNPNKTFKFSYKDLKKRESTKTYIVPDEFNFTPSRYFKFGKFIGATSHFQILASELS
                     DRMLSEFLDIDDNINISFHIRAIDQSEAIKMVKRKNTDIDKMKIEENKKAVRSGYDMD
                     ILPSDLITYGEDVKSLLKDLQTRDERMFVVSIVFMNFARTVQKLDNTIAQISSIANKH
                     NCKLKRLDHSQEQGLVSVLPLGVNKIEIDRGLTSSSTAVFMPFTTEELFINSSNSLYY
                     GLNALSHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILVTDDDVIICDPEG
                     EYGNLVRQFKGEVIKVSSKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGS
                     GLTAEEKSVIDRCLPKIYEKYFEHPTPDNMPILQDLYDMLKGQEEKVGKKLATEMEIY
                     VSGSLNVFNHRSNVDLNKKLLCFDIKELGSQLKKIGMLVIQDQVWNKVSQNRGNKATR
                     YYIDEFHLLLKDEQTASYSVEIWKRFRKWGGIPTGITQNVKDLLMSKEIENIFDNTDF
                     VLMLNQASGDREILARKLKISLPQLRYVTNSNEGEGLLFFGNTIVPFIDKFPKDTILY
                     QKMTTKPEEVR"
     gene            complement(31781..32170)
                     /locus_tag="I9573_000156"
     CDS             complement(31781..32170)
                     /locus_tag="I9573_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAT4794802.1"
                     /translation="MAYVPIPKDLNRVKTKVAFNLTKRQLIGFTLAGLVGIPVYLFMR
                     KFVPNDIAVIFLIVSTLPIFFITLFEKDGLTFEKYFKYIYLHKFYQPQKRVRKEVYLE
                     QEKKNSANKTHAKRKGIEKSKAGLKEK"
     gene            complement(32174..32434)
                     /locus_tag="I9573_000157"
     CDS             complement(32174..32434)
                     /locus_tag="I9573_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT4794803.1"
                     /translation="MNKRKTVFIVVIGAGIMAIIDRIRLHNKVEELEEKTEDIGRCHN
                     DFYLIQQRYNKNTDEQLATIQEEIGSVYEHFEELSKGKEDGR"
     gene            complement(32452..33315)
                     /locus_tag="I9573_000158"
     CDS             complement(32452..33315)
                     /locus_tag="I9573_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794804.1"
                     /translation="MFGIFDKIEEFFKELLLGGIQANLESMFLDINDKVGAVATDVGK
                     TPMGWNGDVFAFIKSINDSVIIPIAGLIITAVLCIELINMVMQKNNMHDTDTFEFFKY
                     IIKMWIAVWLVSHAFEFSMAVFDVAQHMVNKAAGVINTSAVISGDQIVKMVDGLKDKG
                     LGELVMILFETSLIKVAIQVISIVIMLVVYGRMFEIYVYSSISAIPFATMGNKEWGQI
                     GTNYIKGLFAIGLQGLFLMVCLGIYAVLVKTIQITDIHTSTFTILGYAVLLGLMMLKS
                     GTLAKSVLNAH"
     gene            complement(33335..33550)
                     /locus_tag="I9573_000159"
     CDS             complement(33335..33550)
                     /locus_tag="I9573_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT4794805.1"
                     /translation="MEFFAQAVNVLKILVMAVGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANVLK"
     gene            complement(33554..33865)
                     /locus_tag="I9573_000160"
     CDS             complement(33554..33865)
                     /locus_tag="I9573_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAT4794806.1"
                     /translation="MKQEMININANLVAEPTFSSFEKEGEEVEVVNFTLVKKYGKGKE
                     YINCAAYGEKAEKAEDFEKGDLIHVFGYFKKREKEGKTYKNFVVKSYNKIEKKEENEE
                     E"
     gene            complement(34029..35813)
                     /locus_tag="I9573_000161"
     CDS             complement(34029..35813)
                     /locus_tag="I9573_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAT4794807.1"
                     /translation="MIDKILKDIKGLFKVQDKAKFLKQNIPYLAFFYVGNIFSHHVRA
                     YTGGDVIDKIFQGILELNTMSFLPSIHPTDILMGVVVAVLIKIIVYTKGKNAKKFRQG
                     KEYGSARWGTKKDIEPYMDEKFQNNILLTQTERLTMNGRPANPKYARNKNVLVIGGSG
                     SGKTRFYVKPNLMQMHSSYCVTDPKGTIVLECGKMLEDNGYEIKILNTINFKKSMKYN
                     PFAYLRSEKDILKLVQTIIANTKGEGEKSGEDFWVKAEKLYYTALIGYIFYEAPREEK
                     NFATLLDMIDASEVREDNETYMNPIDRLFEALEKKEPTHFAVKQYKKYKLAAGKTAKS
                     ILISCGARLAPFDIQELRDLMSDDELELDTLGDRKTALFVIISDTDDTFNFVVSIMYS
                     QLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIATIRSREISASIILQA
                     QSQLKAIYKDNADTIVGNCDSTLFLGGKEKTTLKELSETLGKETIDLYNTSETRSNQK
                     SFGLNYQKTGKELMSQDEITVMDGGKCIFQLRGVRPFLSDKYDITKHKNYKLLEDYDK
                     KNLFDIESYMKRKGKAKLNRETVITRMQ"
     gene            complement(35810..36295)
                     /locus_tag="I9573_000162"
     CDS             complement(35810..36295)
                     /locus_tag="I9573_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAT4794808.1"
                     /translation="MINEEIARKTLNMEINAGKVTGKVLLNLLKKLMKEAEKFGGLEK
                     LVNANGNEVKLKDMVKKGQLEEIPVEEAELKELKKELNRYGVKFSVMKNKETGKNSVF
                     FQAKDMKVMDKAFKHALSKSEKKAERKESIHKNIEKFKEMAKNSISKEKIKNKQKEQS
                     L"
     gene            complement(36297..37319)
                     /locus_tag="I9573_000163"
     CDS             complement(36297..37319)
                     /locus_tag="I9573_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT4794809.1"
                     /translation="MYLFDEQPIVANKTLAREIGLNEALILQQINYWIEINKKSGKNY
                     HDGKYWTYNSIRAWQENDFDYMSFDTVKRTFSKLEKAGYLLVGNYNKDPRDKTKWYTI
                     NNDKLEELYMDMDLKKQKIQTEALKNSMPNALGQNAPMDKGKISQCNNADCTNAQVQN
                     ALMQKDNLHQPLPENTTNNTTNNTTNNSAYISIHPSIKEKNQLFSENMKGGRIDISYK
                     NIYQNCLEELRHQTGYYEHMSIGNKSVAEEFDEILKILADVMVLDDESFITINQTKIS
                     VQVVKERFRKLNSGHLEYLVGVLRENEYRIRNIRAFVLTAAYNAPSGIDAYYSALVSY
                     DMRGGY"
     gene            complement(37405..37686)
                     /locus_tag="I9573_000164"
     CDS             complement(37405..37686)
                     /locus_tag="I9573_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794810.1"
                     /translation="MRWILKIILFPISIALSILIAFLSFLLGIGTAILYLLMMFCIFG
                     AIASFLQKEVTIGIEALILGFLLSPYGIPMVGTAVIAVLTGVKEAIRSI"
     gene            complement(37705..39339)
                     /gene="rlmD"
                     /locus_tag="I9573_000165"
     CDS             complement(37705..39339)
                     /gene="rlmD"
                     /locus_tag="I9573_000165"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAT4794811.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAEGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVALLS
                     KLDVDKHIDVEIKLDELDLTSAESKATYAQIKEYVLEKFGLKVSTLYIAQIKKKCGIE
                     LREHYNKSKKDNQAIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(39514..41274)
                     /gene="pyk"
                     /locus_tag="I9573_000166"
     CDS             complement(39514..41274)
                     /gene="pyk"
                     /locus_tag="I9573_000166"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005366042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAT4794812.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEILTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(41326..42285)
                     /locus_tag="I9573_000167"
     CDS             complement(41326..42285)
                     /locus_tag="I9573_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HAT4794813.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(42494..46063)
                     /locus_tag="I9573_000168"
     CDS             complement(42494..46063)
                     /locus_tag="I9573_000168"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAT4794814.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(46220..47167)
                     /gene="whiA"
                     /locus_tag="I9573_000169"
     CDS             complement(46220..47167)
                     /gene="whiA"
                     /locus_tag="I9573_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAT4794815.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(47184..47609)
                     /locus_tag="I9573_000170"
     CDS             complement(47184..47609)
                     /locus_tag="I9573_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAT4794816.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(47678..48784)
                     /locus_tag="I9573_000171"
     CDS             complement(47678..48784)
                     /locus_tag="I9573_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAT4794817.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(48796..49653)
                     /gene="rapZ"
                     /locus_tag="I9573_000172"
     CDS             complement(48796..49653)
                     /gene="rapZ"
                     /locus_tag="I9573_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAT4794818.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIDEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(49684..50397)
                     /locus_tag="I9573_000173"
     CDS             complement(49684..50397)
                     /locus_tag="I9573_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAT4794819.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFKLA
                     MKTISDSNLDISLVENIII"
     gene            complement(50401..51315)
                     /gene="murB"
                     /locus_tag="I9573_000174"
     CDS             complement(50401..51315)
                     /gene="murB"
                     /locus_tag="I9573_000174"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAT4794820.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(51588..52358)
                     /locus_tag="I9573_000175"
     CDS             complement(51588..52358)
                     /locus_tag="I9573_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAT4794821.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            52676..>54073
                     /gene="cls"
                     /locus_tag="I9573_000176"
     CDS             52676..>54073
                     /gene="cls"
                     /locus_tag="I9573_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAT4794822.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKE"
BASE COUNT        15649 a        10895 c         6805 g        20724 t
ORIGIN      
        1 gtaaaaattt aaggcttaaa tctttcgcat ctttttgcat atcagctgta gaaacaaatt
       61 gtagatttgt ttttttacca tcattaagct ttatttctct ttcttcaatt atcaaagaat
      121 tactatcact ttctaaatta acatttatat tatctttgat ttgtatttca ttcttattat
      181 ttttttcttt tataaatgct tttatttcac tactcttaga ataaagctcc aaagtttgtt
      241 ctatatattt tatttctttt ccattcatat tactagaaat ttcatttgcc atattatgaa
      301 tcttttcttt tctcgtctcc aaatatgttt taggaaagat aaaaaaaact aatgaatgaa
      361 ctaatataat tattattcca agaacagaaa atatttgtat gaacattttt ggaaatatct
      421 ttaatttttt cattttctct ccaatttata tcctacattt cttatagtag ttatacagtc
      481 taattgtaat ttttttctaa gttcttttat atatacatct attactcgat cataaggagt
      541 ttcttcgcta tctttccata cataatcaat aatttgcatt cttgttaaca cttgtccatt
      601 attatccaat aaacatttta atacttctag ttcttttgca tttacatcta tttcttcacc
      661 atttatttta gctgtatagc tattaaaatt aaccgataga tcattatatt caaacttctc
      721 taaatgtcca taattcttct taattaaaga atctattcta gcctttaaga ccggcaatga
      781 aaatggcttt tctacatatc cgtctactaa attagtaaat gcatcaattt tatattcttc
      841 atcactaaat gcagtcaaga ttagaattgg taaattgctt ttctttctaa tttctttcaa
      901 cacttctaaa ccatttataa aaggtatctg aatgtctaag ataaccaaat ttatatcgct
      961 attaaatttt gacaaggctt ctcttccatc tttagcttga ataacagtat atccaaattc
     1021 tgaaagatat tcacttattc cctctcttat catattatca tcttcaacag ttaatatttt
     1081 catatatacc tccacttaag cctctacact tatattatac agcatattat ttgatattta
     1141 aaagccaaaa aatttttggt gctaaaaagc accgaaaatt ttaattattg tcaattaata
     1201 attcttttgg attccttctt aatatattga atgaagaaat aatcaatgat acaagaagta
     1261 ctaaactcat aaatataact acataaatta tgaatttagg taatacattt atatctaaac
     1321 ttgataatgt cttgttaaat ccttcagctt ctgccccacc acctaattgg ctagatgcag
     1381 attgtctagc tatttgttta gctatatcac cagtaacctt attcaatata ttattcccaa
     1441 gcttatcagc tgtatattga gctaagaaat aagaaccaac gaatgcgggg attgatataa
     1501 ataccatctc aattaggaat tgaccaaaaa tttctaattt tgatatacct aatgatagta
     1561 atactgctat ttctttcttt ctagcattca tccataagaa taataataat gaaactacaa
     1621 ctcctgcaaa tattaatgag ccaacaaata atttatttga aattgagtag atacctgata
     1681 tagattgttg taatgcaggg taatttgatg aacttttaat caaattatat tctctccaat
     1741 ttatatctaa tttcccaaga tcttttatta cactatcaag attcttatca ccttttacaa
     1801 agaatgttgc atcttggtat acagctgtat cttctgtatt accataaact ttagcagctg
     1861 tatgaacatc agtgattaat gtattttcgt atagttcttg tgctgcactt actccaccgc
     1921 tattatgccc atcgaataga cctttaattt ctacttctac tgtttcatct gcacctttct
     1981 cattatcagc atcaaataaa ttagatttta tttttatctt atctccaact ttaagattat
     2041 ttttttcagc taaatcttta tgcattaaga ttttattttt atcttcattt tctaaatgct
     2101 ttccttctac taatttatat gcttctgata caaattttgt ttcttttgat gagtcgttaa
     2161 ccccagttaa cataactgtt cttttaaaat tcttcgctct ttcaggtgat tgattcgcaa
     2221 gagtctcttg agtttcaata atatcataat caactaaatc tgcaacactg tttattcttt
     2281 ttacatagga gtctatacta tctgtttgaa atatcttttt aatatcttca ccttttacat
     2341 ttcccccacc tctaggtgtt cctggattta cttgtctatt tatctccata gaaaaactat
     2401 ttgttatatt agcaaatgtt tcttttgaag ctctgtccgt agcatcttta atagataaac
     2461 taatgagact taaagttgac atagataaaa taactaatat aataattaat gattttaagc
     2521 tttttctagt tacataagca aatgcatttt ttatcattat tcaacctcca aattcatttt
     2581 atttactttt ttcaactttt taccacttag ttctaaaatg atatccgcag aatctgctac
     2641 ttccttacta tgtgttacaa ctattacaca tttatttcta tctttagcta atgtctttaa
     2701 tatattaatt atttctccag cagtaacact gtctagatta ccagtaggct catcagctag
     2761 tattattgga gcatctgata ccaatgctct agcaatagct actctttgtt gctgtccacc
     2821 agataatttc ataacatttc tttttatttg ttttttatct aaacctagtt caaacaagat
     2881 actttcatct gctgatttat ttactaatct aatattttca attggtgata aataatctat
     2941 taaattatag ttttgaaata ctaaagatat attatttttt ctatgattac tatatccttt
     3001 cttttgtata tcttcattct taaacaatat ttttcctgtt tgaggtttat caagtccagc
     3061 aagtaaggaa agaagtgtag attttcctgt tcctgacttt cctactatcg cataaaactt
     3121 cccaagttca aatttttgat ttactcctga caaaactttt tctttagaat ttgcataact
     3181 atatgctaca ttctttattt ctaatatatc cattattttc tcctaactta tttttgataa
     3241 tatttcttta ggcttcttga ttaatattaa tgaagaagca aatacaactg ataaaacaat
     3301 aatacttatt aatattaaat aactttgtcc aagtgttgtt atatttaaca taaaactact
     3361 attatttatt aaacttccac cggaaatcat tgagtcctct gagttaatga atccatctac
     3421 aattactttt agtaatacat ttcctaaaaa taaggaagat attatacttg gtatcgatat
     3481 gaatattaac tcgaatataa attgcattat aatttgtatc ttagatgttc caatagataa
     3541 aaatatacct atttcataaa ttctttctct taaccataga atcaagatta atgaaagaac
     3601 aaccatccca cctaacataa tagaataagt cattattttt attatatgtt ttattccact
     3661 cactgactct aaagactctt caaacgcatt agaatctttt tcaacaaaat actttgactc
     3721 atcaatttta agctctttca atttgtttaa ggctaagtct gtagattctg cactaccaga
     3781 atacattaaa attttatttg cgattttatt attttctgat ttatttaata tctcttggct
     3841 tgttgaataa tctacaaaca ccatattttc actaaaatca gaagataatc ctgtatatgt
     3901 ttcctgtttt ttaccagaaa agatccctat aattttaaat ttatgacttt ttatttttcc
     3961 acttttttca atatctagta attcaagatc aacttcatca cctaatttta gattgttttg
     4021 tttagcaaat tcttcatgaa caataattga attcttatca ttttctccta tatttttgcc
     4081 ttctttaatt gtaaatactc cactactaaa taaaatattt cttttagtat tatttgtagc
     4141 ttcgagtgaa acaacattct taaattcgtc agataaatct tctctattta ttctttgttc
     4201 tccgctaact actttagcat cttttagttt tgctaatcca tcatattgaa ttattttttc
     4261 ttcaatttct tttaattttt caatatcttt aaattgatta acattaaaat atttaccatc
     4321 tttttttgtt attgatattg aagaattaga actttcatat aaagcctttt ctatttcatt
     4381 gcttgatttc attattgtta aacaagaata caagcaagaa agaactatag ttaatatgat
     4441 aaaaattatt aaagttctat ttctctttct tgtaatatat gctatagcat tttttatcac
     4501 tttcctaact actcctctct attttcttac tccataacta attgcatcca ctggacaagc
     4561 atttttacat cgtccacatc tgatacactc cagatgattg caattctcaa ctggatctat
     4621 attcatttgg caaacttttt tacattttcc acaattaata cacttatcct tatccaatct
     4681 atatctaaat attgaaatag ggttaaaaat tgagtaaata gctccaagtg gacatatata
     4741 tttacaaaaa ggtctatata taattataga aagtaacaaa gtaactatta gaatgattcc
     4801 tttccaataa tacaagaaac ctatcgcatt tctcatactt ttatttaata aaacaagtgg
     4861 taatcctcct tccagcatac caatcggaca aataagttta caaaaatacg gagaaccctg
     4921 acctaaaata tcaaccaaaa ataaaggtaa tattattaca aatacaagta atattatgta
     4981 tttcaatttt ctcaaatgtt tatcaaactt aaatgtttca acttttttaa aaaagggaat
     5041 cttatgtagc aaatcttgaa tcaatccaaa tggacacagc catccacaaa caaatcttcc
     5101 caccaatgct cctataaaaa acaaaatccc aactatataa taactaatct tatattttga
     5161 atttccaatt actgcctgta gtgatcctat tggacaagaa ccttttgctc ccgggcatga
     5221 ataacaattc atacctggaa cacaaatttt ctttaaattc ccaccgtaga ttttaccagt
     5281 cttaaaccct tcaaagtaag aattagtaat taaaaaccaa acggattgaa ataaatgcct
     5341 aaaacgatct cttattttat atttatcttt ttttgtacta ccatttataa tatttttaat
     5401 tctatccaat tccaatacac tccatacaaa ttcttatcgc cttatccaag acaactttag
     5461 tttctccatt actatgacca tagtaaatca tcgccatact aataaagata attaataaag
     5521 acaaggcacg gctttttaaa aacttattaa ccatatacca atcaccgctt tacttttatt
     5581 tattttttat tttttccaat tctttttcaa ttacctgctt ccattccttt gaactctttg
     5641 ccccagaata agtatcacct acaatatttc cattactatc aacaaagaaa gtctctggca
     5701 tagcctgtat accattcaat ctaccattaa agtttgtctt atctggcatt aagaaaggat
     5761 atgaagcctt tgtcttttca tgtattaact ttgacttttc aattgcttcc ttgttttcac
     5821 cgttatcatc aacagtatct gtaacaactc caacgatatt tacacccttg cttttcattt
     5881 cattctgaac ctcaaccaaa tcaggaattt cctttacaca agctgtacac caagttgcaa
     5941 atacatttac catagtaaga tcatacttct caaaatcttt acttgtaaaa tccttgccat
     6001 ttatgtcctt tgttgatagt ttacgtaaat ctttaaccga ttctttgtta aatgcttctg
     6061 tattttctaa atcagttttt tctgatagca caaaaccatt tttaggacgt tcttttttat
     6121 ctataatttg aatctcagtt ctttttaatt catccagaag attactttca gctccgctat
     6181 ttgtgcttaa atagcaatcg tattttccat cacttgaaac tcctatttta gtatgagtat
     6241 cacattttgt aattttagat attttttctt ctgatgtatt tttttcgaat ataccaatag
     6301 tcccaattct ttcaagctca ttttgccaat ttttatatcc atcgcccatc ttttctataa
     6361 ctgcattctt ttgttcttct gtcatttttt caaatgtcaa aatagcatac tttagttctt
     6421 tatctatagg actttgatca tctaacatcg ctatcttttt atcatccata tactttttaa
     6481 atttgtctga aagtttaaat ttaagaccta aatattcata aacatattct ttttttgttt
     6541 ttaaagtata atctgatggc ttaaatgttt catttttatc cttctcatta gttgacatac
     6601 ctgcctcttc agaacttata gattgtcctt ctttaatttt attgctttca ttactttttt
     6661 tacacccaac tgtacttaag ctcaaacata acgtaagtaa taggcatgct cctcttgata
     6721 ctattttttt cataaatttt tcctccttta ttcctccttt atttttacac aatagcagtt
     6781 tataactact ttcaataata ttctacacac cttttatgaa acgttgatga aatataattt
     6841 tttatttttc caaactatgg tataataata taaatattat gcttaaagtt gttcttacac
     6901 gattagtata tgatcgatta gtatatgagc aagcacagta agtgtacgga cacttacgaa
     6961 aaaattgatg aagcagccct ctgggatatg cttctttttt tatcaatttt taacttgtta
     7021 gggtataccc taagaccccg aaatacattc aaaggaggat ttacctatgg caaatagaat
     7081 acgaaatgaa agactggaaa ttaaactaac tgaagaagaa aaggctcttt ttgaggagaa
     7141 acgaaagctc gcaaggtgca gaaatatgag ccactttatc cgcaaatgcg ttttggaaaa
     7201 ggaaatatat caagtggatt tagaaccgtt tagagattta cagggcttac tgtccaatgc
     7261 tacaagcaac atcaatcaga ttgcaaagcg tgtaaattcc accggcataa tctataagga
     7321 cgatataaac gatatgaaaa agcagattga atatttctca aaagagttgt ggcaaataca
     7381 ttctctgctt cttaacagaa cttctggagg tgattaaatc ttatggctat aacaaaaata
     7441 catcctataa aatcaaccct taatcttgcc atttcatata ttgttaatgg aaaaaaaaca
     7501 gatgagcaaa tcttagtaag cactcataaa tgccatcaag aaactgctca tacccagttt
     7561 ttaagaacac gaaatgatgt tggaacaaac ggaactgttc ttgccagaca ccttattcaa
     7621 tcctttttac ccggagaagc aagtcctaaa atggcacatc aaatcggtct tgaactgtgt
     7681 aaaaagatat taaaggacga gtacgaattt gtcttatcta cccacataga taaagggcat
     7741 atccacaacc atatcatctt caataatgtg aatatggtaa caggcaagtg ctatcaatcc
     7801 aacaagaaaa gctaccacaa aatcaggtat caaagcgata agctatgcaa agaaaacaac
     7861 ctttctgtga ttgatgagtt ttacgaaagc tataagagaa agtacaaaac aaatggcaag
     7921 tcatggtatg aaaatgagca atctaagaga ggtacttctt ggaaaagcag acttcaattt
     7981 gacattgatc gtcttataaa acagtctaag tattgggaag aatttctaaa aaaaatggcg
     8041 gagcttggtt atgaaataaa gtatgggaaa cacatagcct ttaaaccaaa agataagaca
     8101 agatttacaa gagctaaaac gattggtgag gactatacag aagaaagatt aaaagagcgt
     8161 attacagaaa atcaatctat tacaactcca cctgtcaaaa gacgaatcgg aaatatcatc
     8221 aatatgaatg ccaatgccaa agtgaaagag agcaaaggct atgaatattg ggcaaccaaa
     8281 cataacttaa atacaatggc tgagtcagtt atttttatca gagaacatgg cattaactcc
     8341 attaagcagc ttgataaata cattcaaaaa agtgctgaag aaagacaaga cttacaagat
     8401 aaaatgaaag aaattgacaa ggatatgcag ctactttccg atacaatgga gcaggttcat
     8461 actgttaaaa aatatcgagc ctactacaaa gaatataaag caaacccttc cgataaagca
     8521 ttttttgagg agcataaggc tgaaattaca cactatgaaa tagctctttc aaagctaaaa
     8581 aaatcctatt caaagctgcc gaattcaaag gatattttag ataaacttaa taaactgcaa
     8641 gaaaaaaaga atacccttat tcaagagtat tcttctacaa aatctactat ggacgaactt
     8701 tatcaaatac gaaagaacta tggaatttac atgggtaagg agatggaacg ataactctaa
     8761 tattatatag ctttcttatt tttataatat taaattgaaa tgcctatcaa tgatgcaagc
     8821 atttttatac ctaaagctaa atcaggaatt aaagctacgg aagtagtgat aaaatgcata
     8881 ctttttaaac aagtagtcaa tacttctttc ttcggatgct tctgcttaat ttcatttttt
     8941 atattatcta aattctgatt aagtattgat ttggagttat catcctccat acctttaatt
     9001 aagtcttcta cattgttaat tgccttgatt acttcttcag tattaaattt attttcttgg
     9061 tcagcgttaa tcgtgcttcc gtgttccgca atattgactt gaactccaga actattaaca
     9121 ttgatattat atagagcatt attttcatcg taccccatat cggtagaaat atctttcata
     9181 taattttcaa cctcaagtac aaacggatag accattctat cgccaaacgc ctttgccata
     9241 tcttgatatt tattactgcc agcataatac cagccaaaag tatatgtagg tagattattt
     9301 cctgttataa aggaaaatgc tctatacagt agatacgtcc ttttattact atcattgcca
     9361 agattcaaaa ctttacgtcc ataagaatta cttatcgtat ttaataagtc atctaagcca
     9421 tcaacatcat attctaaact atgcaaatac tcttttatca acaatgtttg ctctatataa
     9481 tttatcattt cttccaaatt tgcattctgt tctccataat atgaattcat aacatttgaa
     9541 gctaatatcc taaattttct actgattttt ctaagttctt ttttactaat ctccatctat
     9601 catctctcca caaaacgggc aataactacc ttctaatttt tttatactac ttattctagc
     9661 ggttttgcca caacacaaat atttcttttc ttcataatct ccagtttttc ttcctatcgg
     9721 aagttctgct tcctttttaa tctcttttcc acgtttaaac tttatacttt tactattcct
     9781 aaaacttttt tcaagattct ttgaaaaatc atttagcatt tcagcaactt gattttcaac
     9841 tattcgttct gctaattcat aaatatcatc gtctaaataa ttctcgtgtg ttaaaccaca
     9901 attaggacac caaatatcta ttgtgctttc agcctcaata tcttccaccg ttaacataaa
     9961 tttttcacta caacttggac attttaattg aacatatccc tcttcatccg ctggaacaga
    10021 aatttcaaat tttatatcat tagccattta aatttcatct cctcacaata aaagttataa
    10081 tttcaccact ggtagactac aaggtcgtgc atctttacgt acaataacaa cactattctc
    10141 agcacgtgta acagcaacat ataatctagc cttactttgt tcttttagag atgtgttatt
    10201 gttaataatc caatcccaca tcggttgagt cggatatata agtactcgtt ggaattccaa
    10261 gccttttgat tctccaaaat tataatatgg atattcatta ttgacggatg tccttttatt
    10321 ttctctcaac tgcattgcat taaatttctg caaatacttc tctacatctg ctttatcaat
    10381 tatcataaca ccatctgatt catcttcagc tctacgcaca aaatttgtag atgttctctc
    10441 tggatacaga ctattcgcaa atttacatat aatatcattg tttctataag tacaattaag
    10501 tgtcgtttca tcaattctta catctaattt tctgcactca ttttttaaaa actcatctat
    10561 tttcccacaa ctatattttt tatatttttt tgatgagtga gttgaatatg tgcattgtct
    10621 aggatccccg accattatca cttcaattct tgaagctatt aaacacttta atatttccaa
    10681 atcatacccc accatatctt ggacttcatc aacatatata cagtcataca ttccactgat
    10741 tctttcaaaa acttttccat tacatttatc atttaactct acaacacact ctgataattt
    10801 atcagtatat accctgttat ctttggcaaa atatttgttg gatgttttct ttacgtacgt
    10861 attgttctgt ccatttacaa atataattcc ttcaattcgt tccttaaatc tacttccttg
    10921 ataaggtcta ataccatctt ttaacaaaaa agaaaaccat gtagaaactg taatattatt
    10981 aggaattaca ccaactttat tcttaattcg gctaattatt tgttgctcat ttgtaatagt
    11041 atatgttagg attagtattt ttttatgagt attaagagaa tcctctataa tttttcttgt
    11101 ttttccagat ccagcagcag ctatatataa tttcccactc atattagaat accctccttt
    11161 atatattgtg gaaattctat agctttatca ctttcaaaaa tctttaaagc aacttctgtt
    11221 ttatgcccct tcatatattt aagtaaatca tctggatctt catatgattt attcaatatc
    11281 tcgtttaaaa tagacatgga attcgatcta tatatacacg gttccaaagt attatagttg
    11341 taattttcca aagaaccatt atattcatgg gtactatcat caaaatatat atttatattc
    11401 tcacaacaat tatctcctaa ataatcgcta tattttctat ttagtgcatc aatatttcca
    11461 tcattatcgg ttatcactga cacacgtaat tctagcaatt ttgcgatttc caaaaatctt
    11521 aaaaaagaaa ttccaaccga tattatatct attccatcat taataggcaa tttaccatta
    11581 tgagagtcca tatatgctct ttgaacaatt agttcgtccg aatcaccttc gactaaaatt
    11641 gctttatttg ataataataa tcgtaacgta tcataacctg caattctttt aaaaaataaa
    11701 tttgtttcat ttgataaatc acctagccgt agtgtttttc ctcgatttag taaaattaaa
    11761 ttttcaagtc ctaatttatt tgatacaaaa ctactatgcg tagtaactat tatctgtttt
    11821 tcttttactg actcatttac tttatctagg agaatattta agtttgaata tgacaaatgg
    11881 cattcaggtt cttctaaaag cacaatctgc ttattttcta ttttatgatc acttaatgcc
    11941 aattgtgttt taacaataca ttgtgtacct ttaccaatgt tattatatgg tatttcattt
    12001 aattgggtta ccaagctgct ttcccatgaa gttttcgtac cgaaatctac agaaattgtc
    12061 aattcgttat ttgatgtttc cgttaatttt ctatttatat tagcaatatt ctcatctcta
    12121 gaaaattgtt ctttcataag cctatgcgct tgcgatatat taataatttc attatcatct
    12181 aacagattct ttactattct cgatatgtat atatctgacc cactggtagg agtatagctt
    12241 gtcgtatcaa tcattgcaga cttaattgga atgctccttg cagttatatt ttctctggca
    12301 aaagacatcc aacttacctc ataatattca attggcaacg atgttatctt gttcttacaa
    12361 agcattttat actcattgct atatttttca tcaaaactaa ttgataaaca aatcccaggg
    12421 gtgtctttag ttttttcaga atttccatca cctaaaaaac ttggcatttc tgcatcaaaa
    12481 tacgcttcaa taattatttt aggaggttct acattcactc cttcgttgac ttctctaata
    12541 tagttttcca ctatattctt attgaaaagg tactgagtaa gttcattcct aatatttttc
    12601 ccatgataag ttccagttaa aaccagatta atagcttcca aaattgtaga tttacctgtt
    12661 ccattattgc caactaatat attaatccca ttttcaaaat caactataaa ccaatctgaa
    12721 aagcatttaa aattctttat ttttaatttc ctaattgaca ttttatcccc cttctttatc
    12781 taatataaaa atcatgattt tagaatataa atattgataa ctatataaat acagttatga
    12841 gtttcctgtt taatcacaat cataaatttt ttatgctaca tcccttaata ttttttcaag
    12901 caataattta acaataagtt ttatgatcct ctagctcctg aaaataaatc tatgactgta
    12961 aacttagact cccattctcc ttacatcttc aataattacg cactccataa tatcttcaat
    13021 atcacaatcc aaagcattac aaattctaag caaaacatcc gtagttacat tctgtccatt
    13081 cttcattttg taaaatgtac ttttactcac ttttgccttt tccatcaatt cattatttgt
    13141 catatctaaa tcaatgagtt ttttgaacaa tcttttataa ctaaaaaccc tcattcttat
    13201 cctccgttca agaaaactat atctagtata ttataccaca aaatatactt ttttcataaa
    13261 tatattctct ttaatcgaac ttgcataaat aaaaaagagc cggtgcagtc ctaagaccac
    13321 accgaccgta aatttatctc tcagcttctt tgcttgcttc tttcttttct ttcggttttt
    13381 ccttatcttc agcctgatat tttttgatag ctccaagtat agattctttc ttttcttcct
    13441 gtcctttcat ctgttccttt gcctttaaca gttttgaaga aagtatcctg atattgctat
    13501 gctccttgcc gttatcatca atggaagttc tgatctgccc aaagagtttg acaaaatctc
    13561 cctgcttaaa gtcctttgga atatcactct tttctccata aacggagcag ttgtgatata
    13621 acttatttcc ctcatcatct ttagatacta cggagaagtt tgctacctta aaggtttctc
    13681 cgttcttatt ttccctttca attacatcaa cttctccaac gatatttccg acaatattga
    13741 taagattatc cttttcttcc cgaacataaa gcaggtcttt tatctgcccc tgtcccctta
    13801 aatcctcaat catatagtca aactcctcat gcagcaaatt aaggctatca ttgtccatat
    13861 aagcgtcata tagcttatct aaagcacttt catcattgat acccttttcc atgcttataa
    13921 tcgctttaac aaagtcttta tgattatcac ttaacatatc ttcaactcta tctctcattg
    13981 ttttgtaatc catgttttta tctcctttca tggtaaaagg gaggttgccc tccctttatc
    14041 ttaaaaattc ctgctctttt attttttgtt tttcttctgt tttactttct tcctgatagg
    14101 ctcttatctg tcctaagata gacggcttat catctgattt ttgtgtggtt tcttctttgc
    14161 tatgaagatt gtcctccaca tcataagttg actcaaaata atcactgtcc ttaaaatcac
    14221 tatcatatct atccggaata ccgtcattat caaggtcttt tgcaaacgga tcatagaagt
    14281 taccctcatc gtcaatatca agtcccgttg ctactcttaa atcttcagaa tcaacataaa
    14341 ccaaatcact aaatgtagat aactcaattt catttttcat atttcttaga gcttcatttt
    14401 ctcccttatt ttcataatca aagctctctg ttttaatagg cgtatcatca atgtactgtg
    14461 tccaagtttt ctcctctaaa ttaagctcat attgaatacc atgtctttca tccggtgtat
    14521 tggtataggc gataccgata tgctttaagt caggatatag agtatgaaac tcatcataac
    14581 tgtgattttc ttcatactct ctgttacaga agtcaatgat tgctcttttt acatcttcca
    14641 caagaggatt atcatttttc ttttcttcca cctctcccat atcgagcagc ttatttaact
    14701 ctgcaagtct taatacctta gttttcaact catctgcctt ttcaaaaggc ttttttaatt
    14761 cctctttggc attttcaagc tgttcttttg tacttatgag cttttcttca agcatattta
    14821 gtttctccgg cattttctca agggcattat ccagtcttgt gatattaccg tccgcacttg
    14881 ttccaagctc tcctaaatgc tttgcaacac cgtttaagct gaaattatga gaattagtaa
    14941 agaagttata gcttacctct aaatccatat ttctatactt tcctataacc ttgctttcat
    15001 tgatttttac ctttgaaata gcttcaagta atctttctcc ggctaatttc ttatcggtaa
    15061 ttttttctcc cgcaatggta atagaagtaa acttttcttc accttccgct ttaggctcta
    15121 cattggatat atcttttttg acagcttcta tgagcttttc tgttcttgcg atttcttcag
    15181 gatagttttt tgccacctta tcctctaatc tgtaacggtt agacttatag tttgcttcaa
    15241 gcattttaag ttttgtaacc tcattatcca aatccatctt ttctttaatc tttggatcac
    15301 ccgttgccaa cgctttaatt tcggcgtaat ttaagctgct ttcgtccaca tcttctgcca
    15361 cacgaacagg agtcttactg gtcataatct gtgaaatgaa cttttgctta ttctctatcg
    15421 tctgccaaag gtaggcatca aaggtattct ctgtaacata acgatagata tttaccttct
    15481 cattttcatt tccctgcctt acaattctac ccgcacgctg ttcaagatca gctggtctcc
    15541 aaggcacatc caaatcatga agtgcaatca gtttattttg cacattcgtt ccggctccca
    15601 tcttctgcgt tgaacccatt aagatacgaa cttcgccttt tcttaccttt gcaaagagtt
    15661 catccttttg cttatcagaa ttcgcttcat ggataaaggc gatttcttct ttcggtattc
    15721 ccattgctac aagtttttct ctaatatcat catagatatt aaattctcca tctccttttg
    15781 gagtagacat atcggagaaa agtagctgtg ttgacctatt ttcttttgtc ttatcccaaa
    15841 tagcaaatac attttttacg catacattta ctttactatc agggttatca ggaagtaggg
    15901 gattgattaa acgctgatct aaggcaagtt tcttaccgtc attggtaatc ttaagcatat
    15961 tatcaacatc cggctctaca accctatttc tgacatcatc cgctctttca gaaaggctct
    16021 ttaggatttc cttttgttct tcagaaggct cagttttaat aacttcataa ttagcttcag
    16081 gtgtaggaag gttaagcata tccgcagtct gaatatctgc tacttccttg aacatactca
    16141 ttagttcagg caagttatag aactttgaaa atctcgtttt taccctatat cctgttcctt
    16201 caggagatag ttcaaaggag ctttgtgttt ctccaaaagt ggaagcccaa gaatcaaaat
    16261 gctctaagtg atttttcttt aaactctcat actgaagata cctttgcatt gtataaagct
    16321 ctgtcattga gttacttaca ggtgttcctg tggcaaaaac tatgcctttt cctcctgtca
    16381 tttcatccat atatctgcat ttcataaaca tatcggagga cttaaaggct tctgactgcc
    16441 cgatacctgc aacatttctc attttggtat agagataaag gttcttgtaa ttatgtgcct
    16501 cgtcaacaaa gagcatatca actcccagtt cctcaaaggt aattacatcg tctttcttaa
    16561 aatcatcatt taatttctca agccttgttt ccagtttttt ctttgtcttt tcaagctgtt
    16621 ttactgtaaa gttctgattt ctgtcatgct tgtattcttc tacatagttt acgatttcat
    16681 taatctgatc ttggatgtgc ttttcctgat attccttact catcgggatt ttttcaaact
    16741 gcgtatgccc gataacaacc gcatcatatt cccctgtggc aatctttccg ataaatctct
    16801 ttctgttctt cggttcaaag tcttttttat cagccaccat aatattagcc gacggatata
    16861 gctgcataaa ttcacgaccg atttgtcctg ttaagtgatt tggaacaaca aacaaagact
    16921 tactgcacat tccaagtctt ttactttcca ttgcggaagc taccatctca aaggtctttc
    16981 cactgcctac cacatgagca agcaatgtgt ttcctccata aagacttctt gctatggcat
    17041 ttctttgatg aggtcttaaa tcaatttctg tattcattcc ctcaaaagaa agattgcttc
    17101 catcatattc tctgtttcgg attgagttaa aacgctcatt atacagtttt acaagacggt
    17161 ttcttctttc ctgatcgtta aatatccagt tcttaaattc ttcttttaat agctcctgct
    17221 tttgtcctgc aagcatggtt tcttttttat ttaagacaga agttttagaa ccgtccggat
    17281 ttataatctg gtcaaatacc tttgtttctt taagatttag ggcatcttca atcagcttat
    17341 aggcatttac ccttgatgta ccaaaggtca tttctgcaag gtcatttcct ttatccctgc
    17401 tttttccctc tacattccat tcacttgtaa tatttgaaaa tttaacctta atatcccatc
    17461 ttgcatatcc cggagttttt aaagtttcaa agatgaattt ttcaatatct ttaatcggta
    17521 tccaagtcgc accaagacgc acattgattt cacttgcttc taattccttt ggaagaactt
    17581 tggtaagctc tgctttttga tattccaaac gattcatttc atagcttatc agctcttttt
    17641 cttttccatt ctcaacaaag ccaagatgag gaagctctcg ttccgtttgt cttaactttg
    17701 caaggtagct gtctacaatg gcaatcttat ctctaatatt tccgcttagg tactcatcct
    17761 ttgttacata gccgtactta tatgaattac tgccgtttgc acaagcgaaa ggcaaatctc
    17821 catcttcaag gttaaaggat aatggtctat aaaagttttg ttcttctctg atgtttagat
    17881 aaatttctcc tctaagttct tctatcaaag ttggtctgtc tttacctgta aggctttcca
    17941 tatactcaaa atccacatag cctttttcag ataccgataa gacaagagct tcaagggaag
    18001 tgtccacatg gtctatgact ttggcttttg taatggttct ctttgagaaa atatccccct
    18061 ttgccttgac gttttcttct tcatcaagaa tttcaatgga agatacaaga gggaaattgc
    18121 tatcctcttt taaggctctg gtattgctta aattatttat aaagccatgc ttctttgaaa
    18181 agttgtcata gacttcatta agcttttctt gagctttctt tacctcatca tcggaatagt
    18241 cctctttctg cttgtagata acatctttaa gggcagtatt cagttcaaga tagtccttaa
    18301 tcttttcctt attcttatcc gttatctctt tcttgatgaa aagggagttt tctctgtaat
    18361 agacttcatc atcaataacg gtataggaaa agtttttgac atcatccgtt gcaggaattg
    18421 aagtgatttc atcatctaaa agctctattt cttcataagt tgatcctttg gatatttctt
    18481 cacttgcttt tgtcagaagc tcttttaagt cggcattttc tttcggcagg cacgcaagtg
    18541 tgtttccaaa tcttcctgaa acttcacgca ttgtgccgag tacctgctca ggatgatcta
    18601 caaaatattt gttataggta agaccatttt catcttcagc aaggtgaatc cagtcctcat
    18661 ctctttctct gatactgtcc cttttcttta ggaatataat atctgaggtt acttctgttc
    18721 cggctatgcc cttaaaggta tcatttggaa gtctgattgc ccctaaaaac tctgctcttg
    18781 ctgcaagata gcgtcttaca ctttcatcct ttttatccat tgttccactt gatgtgataa
    18841 aggcgataat accgccgttt ctgactttat caatggattt cgcaaagaaa taatcatgga
    18901 taaggaagtt atttttgtta tactccctgt catttacctt atattctcca aagggtatat
    18961 tgccgataac cgcatcaaag aagttattgg aaaaggaggt ttcttccagt cctttaatct
    19021 gcacttcact ttcagggtat agtagttttc caattcgacc gcttaccgag tcaagttcta
    19081 tgccataaaa cttggactta ttcatttcat cagggatatt gcctataaag tttccgatgc
    19141 ccattgatgg ttctaaaatg tttccctgtt taaatcccat atccgaaagc gtcttatata
    19201 ttccgtcaat tacggttttc ggtgtataaa agctcgttaa agtagattct cttgcagctt
    19261 cgtattctgc atgtgataag ttctcattta gaaaagccct tgcttctttc cactgacctt
    19321 ccttgctttc atcaaagact tcggaaagtc cgccccagcc tacatacctt gctaaaactt
    19381 cctgagctgt actgtcaagc tctctttgtc cgctctccac tctgttaagc atggaaattg
    19441 cttcaaggtt attatttaac ctttcgcttg gacttaactt ttcagggagc gtttcttctg
    19501 taattttaaa gttatgagct tccgctttcc ttatttgagt ttcctcagta gtctgctctg
    19561 gctgtttata tgtcaggttt tcaaataccc tctcaaggtc gctttccaga cgataaggaa
    19621 tgacatcgaa gcttgttatc atcccgccaa aatactcggt gttgtccttc accgttaccg
    19681 tatttaggtt ctttctcata tcatcaaagc gtgttatggt atggtttttg tccttgtatt
    19741 taacttcatc accgacaata aagttcggtc tttcaagact tattttattc agcaagtcct
    19801 cattatctgt aaagtctaca atcggaattt gatggtttcc acttcttacc ggatcaagcc
    19861 acaaatcata tcttcctgtg atttcatttt tagaaatttc tctgacttta tattcttcat
    19921 gattaaaata gacggtatcg cctgctttaa ctgcaaactc ttctaaaagg ctgtcctctt
    19981 tttcattcag ttctacttct tctctttctt tgaggtaatc aaataaggta gcttgaactg
    20041 tgccacgctt tgtagcttct tctgtttctt caggtgcttc aagctctact ttatcttcaa
    20101 agctaagttc tccattaaag acaaactgaa gctcctgctc atccatttgt tttataagct
    20161 ctatatcttt aagttctcta aaggtctttg gaaaatcctc taaaatgagc ctctgggcat
    20221 ttaattcttt cagttcctca aggttaaaat atccccattc aggctctatt ccaagaacaa
    20281 gtccaaaggc atcaccgctt tccctgtcat actccgtcat ataccaagtc caatttgaac
    20341 gaaaaggaat aatatatgct gcatgaacct gcttatccgc taaatctaca tcctcctgtg
    20401 cgtaaatctc tggcactctt tcaagaattt catcaggcat tagatttcca ggatcatttt
    20461 tagaataata ttgcggttct tttacttctt ctatctctgc cgtactttct gtttcctttt
    20521 cctcaaaata aagtttttta gaaagcagct catcaatatg ctttgcaaca cttgtccaag
    20581 taaggaacac atcattacaa tcattcttct gtaatttaaa accctttgca tcgtgccatt
    20641 catcacttcc cattgcacct gacacaacgt gagaatgtcc tccgattcca tattcatctt
    20701 ttaggaaatt ggctttttcc tgcaaagtat gattttcttt aaaaaacttg gtgattcgtt
    20761 cttttcctct atcaacgccg cttcctcttg aaaggctttc aaggacttca tcttctgtaa
    20821 taaagagctt taccttcgga agttctgtca gattggtact atattccttt cgttgcagct
    20881 caagttcttg caacttctga taaagactat ctaccttgtg ataatgatac cttaatacat
    20941 ttctgctttc tttgtatcct tctaaaaacc tgctatattc tttgattgtt tctttcagat
    21001 attcaggatt ttttaatgct tcagataatc ttttcgtttc ttgcggaaag ccaccacctc
    21061 tttcaaaaga gttgaaatat ccttgctcta ttccttcttc gcttaaatcg tgcgttagat
    21121 accataagga ttctgaaatt ctatcccttt catagtcctg tgcctctaaa agttctacat
    21181 ttgtagcaaa ctcacctctg tcaagaagtt catttatcct acttgctgca tcactccaac
    21241 ttaaaatttg tgtattatcc tctcttgccg atgttccata agctaaatga atacctttat
    21301 ccgaatacca ggaagatact tctctttcat caatgtaaaa tccgtttccg cctttaaagg
    21361 tatctttgag gtattctccc aattcttcta tgctcttgcc ttttgaaaac tcagcaataa
    21421 caggaagcct accgccatca tgatttcctc cgttaataag gacggtatct atatccttct
    21481 cagttagcgg aatagtaagg cttatctgtc cataagaatt ttcgggtaaa gaaaaagaag
    21541 ccttttcagc ttctattatc cctttatccg tattgtcttt taaatctcca ctacttcctt
    21601 gatggtcatt tctttgagag ctgaaatcat cgccccatac tgcggattgt tctcgtcctc
    21661 tatcttccaa gctgctatca actttggctt ctctgctctc ataaactcta ctgcctgttt
    21721 ctgaatatcc atcaggtgtc ctgtcagctt cttctccttg tatagatcca aaagcatctc
    21781 gaagtggctc tgctcctcgc tctgtgaaag gtaattcagc ctcatcactg cgtaggtcgg
    21841 attcggatac ttccttataa agtccgtctg ttcctccaag ctgtttagcg tattctccct
    21901 gattttttct attatctcgt ccgtactctc catttcggag aactcgctcg ttttcatttc
    21961 tttcctgatc atctcgtcga aatacatttt cttctacctc ctctaattct tctttaattt
    22021 tattatatcc cgcttctttt cctattaaaa cttctttttg aagctcaagc tctttgcttt
    22081 tttgaatggt ttcatcaata atctttccgc taatatccga tacactttca ccaaggctca
    22141 ttaaggatat gctgtcaaat ctttgaaaat tttctcttaa aagctcataa tccatcggat
    22201 aatctaactt aaatcttgaa gctaccgcat agcttaccga gtcccttaca aacttggtaa
    22261 atgatattct atcctcatcc gctactctaa gttcattcat taaggtatct attttttcat
    22321 caccgtagag tctgcttaaa gaaaagatgt tttcaagtgt gctttcactt tcctcatagc
    22381 cctcgctttt tatcatttcc tttaagacat cttgatgggc ttctttatca aatctccaaa
    22441 gattgacctc gttgacatcc ctgttttttg aaactgtctg gcttatatca aagatatagt
    22501 ccaccttttt atatgtgccg taatcctcta aaattggtat ccctttctgc cctcgcataa
    22561 ccgttcggtt aaagcgttcc ctccagtagt cgaacttggc acaggctgtc gcttcaggat
    22621 ttttatcata tatacttaat tgacttctaa aatcatacct ttgattgttt ccgacaactt
    22681 ttaggagctt tagatattct gcttcattat acaaaacatc ttgttttatt agctccaaaa
    22741 tgttatgaaa atcatttatt cgcattttta cctccttctt ttttcaaaca aaaaaggcgg
    22801 ttagatttta ctctaatcac cttcgctata taccaaatat tattactcta tttcaatttt
    22861 ttaattgatt cactttcaga tagtatcttc atttgttcaa tagctattgc atttaatttt
    22921 tccaatcttt cagattggct catgccttca tttataaata ccgcattaag attctcaaga
    22981 ttagaaaggc agacaagctg tgatatatcc gcataatctc taatattgcc ttttaaatcc
    23041 ggattttcat cacgccactc cttagctgtt attccaaata gagaaacatt aagaatatcc
    23101 gcttcacttg cataaataaa attgattttt tcttttgaaa gtttatccgg tacaagtttg
    23161 tttttaatgg catctgtgtg aattctataa ttgatttttg ccaaatttct cttaatatcc
    23221 catcctaact tcttttgttc ttcttccttt aggcgttcaa attccttgat taaatataat
    23281 ttaaagtatg gcgataccca agaagcaaac tcaaacgcta tatctttatg agcataagtt
    23341 ccaccatatc ttcctgctct cgcaacgata ccgatagaat tagttttttc cacccattgc
    23401 ttgaccgata atataaaacg gttaaatcct gcttcatttt taattccctc gaattcgggg
    23461 gaattaaaat tcgtgttata catttcttcc caaataccta aaaactctat tgtattcttg
    23521 tttctgagcc acttttcaat caaagcagaa ccgttctcaa tatttctaac catatccgtt
    23581 aaagaaatat aatctctatt gtctatggca ataatgctga tttctgaacc ttcaacattt
    23641 attttagaca tataatcacc tctcaatcat taaattgatt actttgcatt atagcatttt
    23701 tagagcattt tttccacgct gaaattagtg ctgtcttact tgcgatatgt caaaaccgct
    23761 tcttttatct cattttcaat cgcatctaaa aattctttct tagaggattt accctgagca
    23821 atatcagata attccatttc ccactttgct gtcgtttctg ccgacttaaa ggtatctgct
    23881 acaatcgtta caaggctgat tcctttatgc gttgcaatta gattcttctt atctctttcg
    23941 acaaatctct taaagattag attttcaata attcctgccc ttgttgccgg agtgccaaga
    24001 ccttttcttt ccacctctac acctttttct aaggcatcat ttcccgcaat ctccatagac
    24061 tttaagagcg tatcttcagt aaagtgtttc ggaggttggg taaatttttc tttaacctct
    24121 ttattttcaa cgctcaaaac atcaccaaca cttacatcgg gcaatacagc atcttcactc
    24181 ttttttgatt tgtattcttt aaggtatttg ctaaagcctt catccttaat taccttacct
    24241 gaacttgtaa attcaaaacc gtcaaatgaa gctacaatct ttgttgtatt ttcaataagc
    24301 ggatagccta cacttgcatg aaatttatcg gatataagga agtaaacttt tgcttcactt
    24361 aaaggtaatt cagaaacatc ttcttttact gaactgattg tcggtatgat tgcatgatgg
    24421 tccgtaacct ttttagagtt aaatactacc ttgatacgct ctgtatcaaa gtcattttta
    24481 cccaaaatgt tgttgactgt actcacaatc atatcctctg ttaaacatct gctgtctgtt
    24541 ctggggtaag taatcagttt cttttcatac aggctttgag tatagtcaag tgtctgcttg
    24601 gcactatatc caaaatattt attgcactcc ctttgaagcg tagttaggtc aaaaggtaaa
    24661 tcaggctttg taattttttc tttttgaata acatcagtaa tttcaatctt atcgcctact
    24721 aaacttataa gctgttcagc agcaacttca tcatcaattc tgtctgtgga gagtgtaaat
    24781 ccattcatag aaagctctac tgtgtaatat ttttctttct gataatttgc tatctcatca
    24841 tctcttttta caatcatggc aagggtaggt gtctgcactc tgccaacact gtaattttgc
    24901 tgatacagac aagagtagag cctactaata ttcattccga ctaaccaatc cgcaatagct
    24961 cttgcctgtg ccgattcaaa gagattatca tagtcttttc cgtctgttag gttatcaaaa
    25021 ccctctttaa tggcactatc ttccattgaa gaaatccaaa ggcgtttcat cttcttttta
    25081 caattcactt gattatatac aagtctaaaa atactttctc cctcacgccc tgcatcgcac
    25141 gcattgataa ccgtatcaac ctccttatca ttcatcagct ttttaaggat gttaaactgc
    25201 ttcttggttg cctttgctac ttcatacttg tagtcgctcg gaataatcgg taaatcagcc
    25261 atattccact tggcgtactt ttcatcataa gcatccggat ttgccatttg aattaggtgt
    25321 ccaacgcacc agctcaccct atatccgttt ccttcataat atccgtcttt ctttttattt
    25381 gctccaataa cctttgcaat ggagattgca acactcggct tttctgcaat aactaacttc
    25441 atctgtgttt tcctcctgtt ataaatcaaa atagggcgaa agatgttact ctcccgccct
    25501 aactttgcac ccaaaataat atgtttttag gtgcgtttat tcttcatctt ctacttcatc
    25561 tgcatcattt atttcttcct cgctttcaga ttcagaaaag aaatcatcat cttcctcctc
    25621 taaggcttca agttctttat cttctttctt tttcacaacc ttaaaatagt agcctgctcc
    25681 taatgcacca cctactacaa gaagcaaaat aatataggtt cctaaattac tcttttcgtc
    25741 cttcttctca ggctttactt cttccttaac aggttcttct tttactactt cctgctttgg
    25801 tgcttctttt ttctccacca tgttaagcaa atcatcttca gatacttccg taagaagcat
    25861 tacattctca ctatcttcat catgattgat gattaaatga aaagtcttac cgtttttcgt
    25921 ttgaaaggta ataaactgcc ttgcatctgc cgaatactga tccgtttctt tgttatcgct
    25981 gctatctcca tgatgaatcg gatactcctt atttgcatta tctttattct ccgtaacgga
    26041 tgctctcgcc tttgacggag atgaagctac tcccttgttg gtgtttacac tcttacttgc
    26101 agaccccatt gatgaatcct gactgttatt cgcaggagct tttgccgtca acttattggg
    26161 atagcgaact tccttttctt tctcctttac ttccgttttg cctttaccgg tatcctttgt
    26221 agtattacct gagcttaccg aatccgatga gcctttaccg gtactactta cagaagtctg
    26281 tggtgtttga ggagaaatac ctgaacttgt cttaattccg gaaataggac taatcggtgt
    26341 aactgattgt gatgttacag gagctttatt tgtttcagtt gcctttttct caagctcttt
    26401 caccttttcc gtcagcttat ctatttcctt tttcatatct tctgataagt ccttatttcc
    26461 cttatctttc ttcatttttt ctttcaggct atcaatttca tcttcaaggt ctttaatttt
    26521 attcttttgc ttatcactta atttgtcttt gtccttaagt tcgcaattta acttatcaag
    26581 tttatcctga agctcttttg cctctttttc tatttttgaa atatcgtctt tagaaagctc
    26641 tgtctgcgtt cccttatctt ctgtctgagt gcattcatcc tgctttggct tttcttcttc
    26701 cgtctgcgtt tccttatcct taacctccat ctttgctacc ttacggataa tctcatcttt
    26761 tccatctccc tttacctcat aaaacagaaa ttcatcaatg aatttcatat catctgggag
    26821 cattggaaga tctccgctat caataagttg tcctttctca gcctttatct tttcttcctt
    26881 aaagaccttt tcatcttcaa agatatacct tacatttact tcaacttccg tttgaacagc
    26941 ttcgtttttt gttggagctt cagatttttg ctcctgtgca taaacaaccg tccaaagacc
    27001 taaaatacaa ctaatactta caattaccgc aagtgctacc gtccaaaact tattattctt
    27061 tttcaaactg gttttcattt gttttctcct ttttcatttc tgctttttgt ctgtttacct
    27121 ttgctatcaa atcgctgatt gtgatgttgt tctttctgca aatggcaatg atttcttcat
    27181 tttcaagctc ttcttcacga atgaataaag gctccaattc ttcatcaatc agagcctttt
    27241 tatcctttaa cttctttatt ctgtttttta ctgttgtaag ttcctttttc aaattgttac
    27301 ctcctatttc tttacatttg gcggaaaacc gaatcctacg ggatggtgct tacaaaatgt
    27361 tgctatccaa tcatctaagg tagttactct tattcgaccg atgttatcaa tcacttttcc
    27421 atctccaatg taaataccta catgaccata ggtaagccct gcactgcttc cgctactgct
    27481 gctttcaact gccacaagca ttcccacttt tagctttgac ctgtctgatg taaaggtata
    27541 gtttcggtac atatcacagg cattcccgcc tatatatcca agtcctgcat tttgatagac
    27601 ctgtgatacc cacatggcac accagcctgc acccggagat ggcgtaatgt atgcagcatt
    27661 aacaatcttc ttttgtactt ccgtagatgc ctcatactct tttccaccgc cgatacctcc
    27721 atttgcactg ataaggtcag aattaccaaa ggcttctctc atattgcctt gtgcaaggaa
    27781 caaggcttca tagtgcttta gattatcggg atagtttgca aagacctttc ggataatact
    27841 gtccatctct tttttatgaa gcgttacgat gagtttttta tactcataag gttcttcgtg
    27901 gctttcggta tattcattgc catcttcatc ggtataggtt tccgtaaccg tcctgtatct
    27961 gatttcaatt tcttccctat actcaaggtc atacatggac tcaaataatt cttttaatat
    28021 ggattctact tccgatacac tctttacctc tccgcacctt gatgtaatgt aggataaaag
    28081 ctcatggaca ttatgaccga tgtattctgt attatttaag atatattcat catatccagg
    28141 ataattttct ttcacatagt caacttcact ttgaagctca ccctccatat ctgaaaattt
    28201 ctgattgatt tcacttagga catttggctt tgataaatag cttgttgtaa gcacagagct
    28261 tgtagagttc ataaagcctg tcattcccgt tcctgcaaag ttaatcacaa aagtccctaa
    28321 gataatgata cctatgaaaa taagcattag tccttttgct tttcggataa tcatatcctt
    28381 tgagcttttc agtgttccga taagtccctc tttaattcga tctctaagcc ttgacttatt
    28441 ctcacgagaa attgctgcct ttacctgatt tttcttttga aatcttttat atgcacttgc
    28501 tcttttatac tcatccgtct ttttcagttc ctttttggca tcacgaaatt ccaactttga
    28561 cttacgcttt ctgattttat agtccttatt tgtaaggtca tagcctcttt tagctctttt
    28621 cttatcagag tacttcttta tgccatgaat gagcttggag cttgtatctg ctgtcttttc
    28681 tcctgcttcc actccttgat tttcatcact tccatgagac aagtaatctc ttacagtttc
    28741 acttccttta gcaagtcctg aaagggcaga tacctttacc atgtcttttt ttagcttttt
    28801 cttttggtct tttgataggc tgctataaac ttttccgctg acagcatctt ttcccgcctt
    28861 tttatcatca gccttatttc cctttggtgc atcgtccttt ttccttgtat aaaggctttc
    28921 agaatagttc ttcctcttgt acctgctttt ttgagtctta tgatttttaa aggaattttc
    28981 taatgatttt tctttatggg aaagattatc ctccacatca taggtcgatt caaaatagtt
    29041 gctgtctcta aagtcattat cgtatctgtc tataatcccg tcattatcaa ggtcttttcc
    29101 taaaggatca tagatttttc catccttaac ctccgtaata taatctgatc ttcccgtttc
    29161 aattgcggaa tttacgtctc ccttatctga agttctatat ttttcgtttc tcttggatgt
    29221 tccataagac ttttcattat cagcacttac cttacttgtt ttttcatgca ctttatcctg
    29281 aaaccgttct ttctcatgaa cgattttacc tctgtaatca tcgttatgtt ttagcttact
    29341 ttcttcaggt atcgtatctg ttttactatg aaacatctct ctttcaaggc ttgccttatg
    29401 cctttcctta aaatcctttt tcagcttctt tcccataggc tacctcactt cttccggctt
    29461 ggtagtcatc ttctgatata aaattgtatc tttcggaaac ttatcaatga aaggcacaat
    29521 ggtatttcca aagaataaga gtccctcacc ttcatttgaa ttagtaacat atctgagctg
    29581 tggaagtgag attttaagtt ttcttgcaag tatctcccta tctcctgatg cttgattaag
    29641 cattaagaca aagtctgtat tgtcaaagat attttctatc tccttactca taagtaggtc
    29701 tttgacattc tgcgtaatac ctgttggaat acctccccat ttacgaaatc ttttccaaat
    29761 ctctacggaa taggatgctg tctgctcatc ttttaaaagc aaatggaact cgtcgatata
    29821 gtaccttgta gccttgtttc ctctgttttg tgacacctta ttccacacct gatcctgaat
    29881 aacaagcata ccgattttct ttaattgact tccaagctcc ttaatatcaa aacaaaggag
    29941 cttcttattt aaatccacat ttgacctgtg attaaagaca ttgagagacc ccgatacata
    30001 gatttccatc tctgttgcca gcttcttacc gaccttttct tcctgccctt ttaacatatc
    30061 gtataggtct tgaagtatcg gcatattgtc aggagtagga tgttcaaaat acttttcata
    30121 gattttagga aggcacctat ctataacgga cttttcttct gctgtaagac cactaccgcc
    30181 tactacaagc tcaagcatac tcatgataaa gtttgccttg tctttcagcg gtgcatcccc
    30241 atcaccgtag ttcatgttta tatcaagagg attcaggtaa tccttcgact tactgctgac
    30301 cttaatgact tctcccttaa actgccttac gaggtttcca tactctcctt ccggatcgca
    30361 gataatcaca tcatcatctg ttactaaaat cgcatttgcc atttctcttt tggcactaaa
    30421 ggacttaccg ctgcccggag ttccgaggat tagaccgttt gggttcttta atttctttct
    30481 atctgccata atcaggttat ggcttagggc atttagtccg tagtaaagac tattacttga
    30541 attgataaaa agctcctctg tggtaaatgg cataaatact gccgttgatg agcttgtcaa
    30601 tcctctatct atttcaatct tatttacacc aagaggcagc acgctcacta aaccttgttc
    30661 ctgagaatgg tcaagtcttt tcagcttaca gttatgctta tttgcaatgg agcttatttg
    30721 agcaattgtg ttatcgagct tttgcactgt ccttgcaaag ttcataaaga cgatacttac
    30781 cacaaacatt cgctcatctc ttgtctgcaa gtcttttaag aggctcttta catcctcacc
    30841 ataggtaatt aagtccgatg gaagaatgtc catatcataa ccgcttctta ccgccttctt
    30901 attttcctca attttcatct tatcaatatc agtgtttttt cgttttacca tcttaatggc
    30961 ttccgattgg tcgattgccc taatatgaaa ggaaatattg atgttatcgt caatatccaa
    31021 aaactcggat aacatcctat ctgaaagctc actggcaagg atttgaaaat gacttgttgc
    31081 tccgatgaat ttgccgaact taaagtatct actcggtgta aagttaaact catcaggcac
    31141 aatatatgtc tttgtgcttt cccttttctt taggtctttg taggaaaatt taaatgtttt
    31201 atttggattt aaaacatcat gaagtatctt tagcctttct tctccgttta ggctttctgc
    31261 tcttactccc atacttttaa gactcgataa tatatctatc tccagtcttt caagttttga
    31321 tgttgcctgt tctaaattat ccgcttccac tgtaaaggtt acatactttg attttttcaa
    31381 tccgttattt ccctttacaa tctggctttt aagcatctct ctaaattcaa agcgtatatc
    31441 gtcgaaaccg tcctttttat ccggtatctg aattgccgac ttcatttctt cgtttcgtcc
    31501 gagctgattg atatatgaaa actcaatgct gatacttgga tcaaaagagt ttaggaagtt
    31561 tgcaaattga ttaaaaatca agtccctatc ttcatctaag gcaagctgat agttaatgtc
    31621 ctgaaaggct atacttttac tgaagtgctt ttcatcaagc tggcatattc cgctttttaa
    31681 aagccttaga tagggaatgg tatcttctac cgtatatctt ttcggttctt tcttaaagat
    31741 aaggtcaagg attcctccct tatctttttt tgacttgcaa ttacttttct ttaagtcccg
    31801 cttttgactt ctcaatgcct tttcgttttg catgagtttt atttgctgaa tttttctttt
    31861 cttgttcaag gtaaacctcc tttctcactc tcttttgcgg ttgataaaac ttatgaagat
    31921 aaatgtactt aaagtatttc tcaaatgtca gtccgtcttt ttcaaatagt gtgatgaaaa
    31981 agataggaag cgtggatacg ataaggaata tgacagctat gtcatttggc acaaacttcc
    32041 tcataaataa atagactggt attccaacca gtcctgcaag tgtgaagcct atgagctgcc
    32101 ttttcgttag gttaaaagca acctttgtct ttaccctatt taagtcttta gggattggta
    32161 catatgccat atcttacctc ccatcttctt taccctttga cagctcctca aagtgttcat
    32221 atacagagcc gatttcttcc tgaatagtcg caagctgttc gtctgtgttc ttgttatatc
    32281 tttgctgtat taggtaaaaa tcgttatggc agcgaccaat gtcctctgtt ttttcttcca
    32341 attcttccac cttattatga agcctgatcc tatcaattat cgccataata cctgctccaa
    32401 ttactactat aaatactgtt tttcttttgt tcatattacc cttcctttct tttagtgtgc
    32461 atttaatacg cttttggcca gtgttccgct ctttaacatc attaacccta gcaaaaccgc
    32521 atatccaagt atcgtaaagg tacttgtgtg tatatctgtt atctgtattg tcttaactaa
    32581 tactgcgtat attccaagac aaaccattaa aaagagtcct tgtaaaccta tggcaaacaa
    32641 tcctttgata tagtttgttc caatctgtcc ccactctttg tttcccattg tggcaaatgg
    32701 aatggctgaa atggatgagt aaacataaat ctcaaacatt cttccgtaaa ccacaagcat
    32761 aatcacaatt gaaatgacct gtattgccac ctttataagc gaggtttcaa agagtatcat
    32821 gacaagctct ccaagacctt tatcctttaa tccatctacc atttttacta tctgatctcc
    32881 ggagataacg gcagaagtat tgattacccc tgccgcttta tttaccatgt gctgtgccac
    32941 atcaaagacc gccattgaaa actcaaatgc atgtgatact aaccatacag caatccacat
    33001 cttgatgatg tacttgaaaa attcaaaagt atctgtatcg tgcatattgt tcttttgcat
    33061 taccatattg ataagctcga tacaaaggac tgctgtgatg attagccctg ctatgggaat
    33121 aatgacggaa tcgttaatgc ttttaataaa ggcaaataca tctccgttcc accccatagg
    33181 agtttttcct acatctgttg caactgcacc aactttatcg ttgatgtcaa gaaacatgga
    33241 ctctaagttt gcttgaatac cgcccagtag aagctcttta aagaattctt ctatcttgtc
    33301 gaatattcca aacatctaag ctctcctttt cttattactt gagtacattt gcaagaagcg
    33361 gaatcagctt aagtccgata aggacaatgc ctcctcccgc cataagctgc ttgatgccct
    33421 gagatttcgc tcccggatta tcattaccat atccttccat aaggttaata actccccatg
    33481 ctccaagtcc agcacctact gccataacta aaatctttaa tacatttact gcctgtgcga
    33541 aaaattccat aatttattcc tcctcatttt cttctttctt ctcaatcttg ttatatgact
    33601 tcactacaaa gtttttgtaa gtctttccct ctttttcacg cttcttaaag tagccgaata
    33661 catgaatcaa atcgcctttt tcaaagtcct ctgctttctc tgctttttct ccgtaggctg
    33721 cacaattgat gtactccttg ccctttccat atttttttac aagcgtaaag tttacaactt
    33781 ctacttcttc accctctttt tcaaaacttg agaaagtagg ttctgccact aagttggcgt
    33841 tgatgttaat catttcttgt ttcatctttt taaatttctc cttttcattt caataaaaaa
    33901 aacgactgga gtttttcttt tccaatcgct gatactctta ctatttagtt ttcgtttaag
    33961 tgggatttct gatccttatc atcttgcctc ctgattttga ataaaaaaac agcaaatctt
    34021 tttaagactt actgcattct tgtaataacc gtctctctat ttaattttgc ttttcctttt
    34081 cgcttcatat agctttctat gtcaaacaaa ttcttcttgt cataatcctc aagcagctta
    34141 taattcttgt gctttgtaat atcgtattta tctgaaagaa aaggtctgac acctctaagc
    34201 tgaaatatac attttccacc gtccattaca gtaatctcat cttgactcat cagttcctta
    34261 ccagtctttt gatagtttaa accaaagctc ttttgatttg accttgtttc cgacgtgtta
    34321 tacaggtcga tagtttcttt tcccagtgtt tcagacaatt cttttagcgt agttttctcc
    34381 ttgccaccaa gaaacaaggt actatcacag ttacctacga ttgtatcagc gttatccttg
    34441 tagattgcct ttagctgaga ttgtgcttga agtatgatac ttgctgaaat ctctcttgaa
    34501 cggattgttg caattaactt ctcaaactta ggaattaagc cgatattagc gaactcatca
    34561 agtagacacc tcacatggac aggtaatcgt ccgccatata catcatcggc tttatcacat
    34621 aaaagattaa ataactgtga gtacataatg gatactacaa agttaaatgt atcatcggta
    34681 tcggagataa taacaaatag tgctgttttt ctatctccca gtgtatcaag ctcaagttca
    34741 tcgtcactca tcaagtccct aagttcctga atatcaaagg gagcaagtct tgcaccacaa
    34801 gaaataagaa tcgatttggc agtttttccc gcagccaatt tgtacttttt atattgcttc
    34861 actgcaaagt gtgtaggttc tttcttctcc agtgcttcaa agagcctatc tatcggattc
    34921 atataggttt cattatcttc tctgacttct gaagcatcaa tcatatcaag cagagttgca
    34981 aagttctttt cttctcttgg agcttcatag aagatatagc caataagtgc tgtatagtag
    35041 agtttctcag ctttcaccca aaaatcttca cctgattttt ctccctctcc cttggtgttt
    35101 gcgataattg tctgtactaa tttgagtatg tccttttcac ttctaagata agcaaaggga
    35161 ttgtatttca tagacttttt gaagttaatg gtatttagga tttttatctc atatccgtta
    35221 tcttcaagca tcttaccgca ctcaaggact atagttcctt taggatcggt cacacaatat
    35281 gacgagtgca tctgcataag gttcggtttt acataaaatc tcgtttttcc acttcctgat
    35341 ccaccgatta caagtacatt cttatttctt gcatactttg gatttgccgg tctgccattc
    35401 atggttaatc gttctgtttg cgtaagtagg atattgtttt gaaacttttc atccatatat
    35461 ggctctatat cttttttcgt tccccatctt gctgagccat actctttccc ctgtctaaac
    35521 tttttagcat ttttcccttt ggtatatacg ataattttga ttaaaacagc tactaccact
    35581 cccattaaaa tatccgtagg atgaatgctt ggaagaaagc tcatggtgtt aagttccagt
    35641 ataccttgaa atattttgtc tattacatcg ccaccagtat atgctcttac atgatgagaa
    35701 aagatattgc caacatagaa aaatgcaaga tagggaatgt tctgctttag aaactttgcc
    35761 ttatcttgca ccttaaataa gcctttgatg tcttttagta tcttatctat cataggcttt
    35821 gctccttttg tttatttttg attttctctt ttgaaataga gtttttagcc atctctttga
    35881 acttctcaat attcttgtga atagattctt tcctttctgc tttcttttct gattttgaaa
    35941 gagcgtgctt aaaagcttta tccatcactt tcatatcttt ggcttgaaag aatacagagt
    36001 ttttacctgt ctctttattt ttcatcactg aaaatttaac accgtatcta ttcaactctt
    36061 ttttaagttc tttaagctca gcttcttcca ctgggatttc ttcaagctgt ccctttttaa
    36121 ccatatcttt tagctttact tcatttccgt tggcattgac cagtttttca agtcctccga
    36181 atttctccgc ttctttcatc aattttttta ataagtttaa tagaactttt ccagtaactt
    36241 taccagcatt tatctccata tttaaggtct ttcttgctat ttcttcattg atcacatcaa
    36301 tatcctcctc tcatatcata gcttacaaga gccgaataat aggcatctat tccacttggt
    36361 gcattataag cggcggtcag gacaaaagct cttatatttc taatcctata ttcattttct
    36421 cttaatactc ctactaagta ctctaaatgt cctgaattaa gtttcctaaa tctttccttt
    36481 actacttgta cagatatctt tgtttggttg atagtaatga aagattcatc atctaatacc
    36541 attacatctg ccagtatctt taatatttca tcaaactctt cagccacact cttattccca
    36601 atactcatat gttcataata tcctgtttgg tgtcgcaatt cttctaaaca attttgatat
    36661 atatttttat agcttatatc tatcctccct cccttcatat tctctgaaaa taattgattc
    36721 ttttctttga tggaaggatg gatggaaata tatgctgaat tatttgtagt attatttgta
    36781 gtattatttg ttgtattctc tggtaatggt tggtgcaaat tatccttttg cattagtgca
    36841 ttttgaacct gtgcattagt gcaatctgca ttattgcatt ggctgatttt gcccttatcc
    36901 attggtgcat tttgccctaa tgcattgggc atagaatttt ttaatgcttc tgtttgtatt
    36961 ttttgttttt tcaaatccat atccatatat aattcttcaa gtttatcatt attaattgta
    37021 taccattttg ttttatctct tggatcctta ttgtaatttc caactaaaag ataacctgct
    37081 ttttctaact ttgaaaaagt tctttttact gtatcaaagc tcatatagtc aaagtcattt
    37141 tcttgccatg ctcgaattga attatatgtc cagtatttac catcatgata attctttcct
    37201 gattttttat ttatttctat ccaataatta atctgctgta gtatgagtgc ttcgtttaat
    37261 cctatttctc tagcaagagt tttattcgca actataggtt gttcatcaaa taaatacata
    37321 actcatcaaa cactcctttc tttttttcaa acaaaaagac gatagttatt tctatcgtct
    37381 aaaatcatat ataaatatca ttttctaaat agagcgtatt gcttctttca ctcccgttaa
    37441 aacggctata acagctgttc caaccattgg tattccgtat gggcttaaca aaaatcctaa
    37501 tatcaatgct tctatcccta tggtaacctc tttttgtaaa aaagatgcta ttgctccaaa
    37561 tatgcaaaac atcatcaaca ggtaaagtat tgctgttcct atgccaagta aaaatgaaag
    37621 aaatgcaatg agaatactta acgctatact aattggaaat aagataattt ttaatatcca
    37681 tctcatatat actctccatt tatactaaat cattttaaaa tgtcttaaag cgtccattat
    37741 agcttcttct ttttcaggcg tacactgtgg gatagcctga ttgtcttttt tagatttatt
    37801 ataatgctct ctcagttcaa ttccacattt cttcttaatc tgtgcaatat aaagtgtaga
    37861 aacctttagc ccaaattttt ctaatacata ctccttaatt tgagcatatg ttgctttgct
    37921 ctctgcactt gtcaaatcaa gctcatccag cttaatttca acatctatat gcttatcgac
    37981 atcaagtttg gacaataacg ctaccgtctc cacatgtgta gaatgaggga acatatctac
    38041 aggttgtgcc tcaacacatt taaatccgtg aacatttaga tagtctaaat cccttgctaa
    38101 agtagaagga ttacaagaaa catagacaat tctttccgga ttcattgaaa caatagtctc
    38161 taataccttt tcgtcacatc cctttctagg tgggtctaca actactacat tggcagtctt
    38221 accctctcta tacatcttag gtacaatttc ctctgccttt ccaacataaa attcagcatt
    38281 atgtatattg tttaatttag catttatctt agcatctttt atggcatcat ctataatttc
    38341 tattccataa acctttttag ccttttgtgc caaaaataaa gatattgtac ctatcccaca
    38401 gtaaatatca aaaacagtat cagtatcatt taaatttgca tattcaagag ccttatcata
    38461 caatacttta gtttggacag ggtttacttg gaaaaatgat aatggtgaaa tctcaaacac
    38521 taagtcacct atataatcgt ttatttttcc atctccatat ataactatat tttctcttcc
    38581 tagaatagca ttcgtatctt tttgatttac atttacaacc aaagtcttaa atccaggtat
    38641 attttcctta agcactgaag ctaattcttt aagatatgga agtttgtttc catttgctac
    38701 taatacaacc attacttctt tagttgtaaa acctattttt gttactaaat gcctaatcaa
    38761 ccccgtgtga gtcttttcat catatatact taccttatag gcccttatat atgtttttat
    38821 tattttaaca attttatcat ttatctcatg ttgtattacg catttttctg tcgatattac
    38881 atcatgagtc ttctttttat agaagccaat aattggaata ccattattct tttgtattgg
    38941 aaattgtgct ttatttctat atctatatgg attatccatt cctatagtgt catgaacttc
    39001 tacatcctct aacttaccta ttctcgaaag tacttgttta acttcatttg ttttcatttc
    39061 aagttgttta ttataatcta aatcttgtat ctgacaccct ccacaatccc taagattgtc
    39121 actacacact cttttcaccc tgaatggaga cttttctatt atctcaacaa tatctccctc
    39181 agcataattt ttctttactt tattaatcct aactttaact ttatcatgta ttaatccgcc
    39241 ctctacaaaa actgtaatac cattgtactt tcctactcct actccgcctt gaccaatgtc
    39301 tactatatct acaatataca tctcgttttt cgaaagcata aatcaccctc caatcaatct
    39361 attatattta aatctttaca aacttttttc tttaaattta tattaaaata ttgctattaa
    39421 ttaaactata ttttttacta agctacaata ttttatttta ccaaaaaaaa aggagcttat
    39481 aaagcccctt agtaaataaa tttattattt ttcttataaa actctagttg aacctctata
    39541 taccacgcct cttgatgcat ccacagttac aacttctcca tcttttacta gatttgttat
    39601 atcagttgca gacacaataa ctggtatatc taaatttata cctacaatag ctgtatgtga
    39661 tgtcattcca ccattctcag ttactacagc tcctgctttt tctatataat tgttcatttc
    39721 agcatcagta gcagtagaaa caagtatgtc gccttcatta aagtctacac aagcatttcc
    39781 attctttatt atacgaactt taccttctac cgtttgagta cctacaccta taccttgcac
    39841 gatttcttcg cttattacat gaacctttat taaattagta gttccactta ctcctactgg
    39901 tactccagca gttataacta ctaattcccc attatctata taatcagctt gtctagctgc
    39961 ctcaattgcc ttttctataa cttcatctgt atttcctgca acagaagact taactggata
    40021 tactccccat gtaagagcta atttattcat agtcttttca ttatttgtag tagctattat
    40081 tggagatttt ggtctaaatt tagataccat tctagcagta tatcctgaag aagtagatgt
    40141 tattatagct gatgcattta aatctactgc tgttgtacaa gttgcatggc ttattgcatc
    40201 tgttacagta acattgttag taccattttc ctttagtaat ctatcataat ctaaagtctc
    40261 ttctgttctt tttgctattg tagccatcat ttttactgcc tctacaggat attttccagc
    40321 agcagtttct ccagataaca ttatcgcatc tgttccatca tatatagcat tggcaacgtc
    40381 tgtaacttct gctcttgttg gtcttggatt tcttatcata gaatcaagca tttgagtggc
    40441 agtaacaact ggctttgcta attcattaca tttttttatc atcatttttt gaactattgg
    40501 catctcttca gttggtatct caacacctaa gtcacctcta gccaccatta taccatcaga
    40561 aacttttaat atttcatcta ggttctctac accttcttgg ttttctattt tagatattat
    40621 ttgtatatca gttgcattat tgttctctaa tacttctctt attgctagta catcagatgc
    40681 ctttcttaca aaagaagcag ctatataatc tattccttga cttattccaa actctatatc
    40741 acttatatct ttaggagtta ttgctggtaa atttatctta acaccaggta catttactcc
    40801 tttatgattt ttaactattc ctgaattttc aacaacacaa actatatctt ctccatttat
    40861 ttcttttact cttaagccaa ctaaaccatc atctattagt atagaatctc ctgatttaac
    40921 gtcttcagca agacctttat agcttactgt acacatttct ttagtcccca taatatcttt
    40981 catagttata gtgaactttt gcccttcttc taaaaatacc tctgggtctt caaagttacc
    41041 tgttctaatt tctggacctt tagtatctaa taatatagca actggcttat ttaatttttc
    41101 tctaactttc tttgccatat caattctttc tttatgttct tcatgcgaac cgtgagaaaa
    41161 gttaaatcta cacacattta gaccattttg cataagttga gttaaaattt cctcactttg
    41221 tgatgctgga cctagtgtac aaacaatttt tgtcttcttt acattattta acatatttgc
    41281 tcctcctgag atattattcc acagatttat ttttaatact aaaacttata tagataatat
    41341 ttttgccatc tcataagctt ctttatcaaa tactttcttt tgagctaaag cttcacttat
    41401 ttctaaatca attattttgt tttcttttat tcctacaact ctagctgatt ttccatctaa
    41461 taataattct actgctctta ctccaagtct acttgctagt attctatcag atacagttgg
    41521 gcttcctcct ctttggacat gccccaatac agtgactctt aaatctgctc ctgactcttt
    41581 tttaagttcc ttttctaaat cactagcact tccgactcct tctgctaaaa cgattatact
    41641 atgtctctta cctctttttt gagtagtctt taatcttaga gcaacttcat ctactgtaat
    41701 ttctacttct ggtactatta tagtttccgc tcctcctgca agacctgcat ataaagctaa
    41761 atctccacag tgtcttccca taacttctac tatgttaact ctttcatgag atgatgatgt
    41821 atctcttatc ttacctatag catctataat agtattcatt gcagtatcaa atcctatagt
    41881 ataatctgta tatgctaaat cgttatctat agttcctggt atgcctatag ctgggaatcc
    41941 taagtcacta agtttttggg ctcctgcaaa tgaaccatct ccaccaatta caactaagca
    42001 atcaatttta tactttttaa gaatttttac tgcttttaat ctaccttctt ctgttttaaa
    42061 ttcttcacat ctggaagatt ttaatatagt tcctcccctg tgtataatat ctccaacaga
    42121 agataagttc atttcagtta aatcttcttc tagtaatccc ttatatcctc tattgattcc
    42181 atatactttg catccataat atattgctga tcttacaacc gctctaatcg cagcattcat
    42241 tcctggagca tctcctccac ttgttagtaa tcctatagtt ttcatttttt acctccttgg
    42301 attttatgtc aattttttat tataatcatt gttattgtat tctaatggac tttttttgtc
    42361 cctatatcac tttttaatcc catcaagact aaaaaaatta aatgtcctta aatgtccatt
    42421 ttgtgatatt atatcacaaa acggacaata tttttcatat tattataata tatttagatt
    42481 tttatataaa aaatcaagct tttactttta tgttatcttt aggaagtaac tcttctaagt
    42541 tatttataaa ttcatcacat atacaagtca atgccccggt cattttatat gctttacgtt
    42601 taccatttat attttcatcc atagggtaca agtatatact gtcattacct ggatattgtt
    42661 tggctatttc tacaatatta ttagttgtat cagtatcaga catactatct attttcaaat
    42721 acaatttttt accactttct ggcttaatat tttctacatt tctagttctt cctgaattgt
    42781 agtttctatc tgcaaagtca gtatcatcat ttatatcttt tatctctcta gctataagtt
    42841 ttgcattttc gccttctttt atacttaatg ttccttttac atatattata ttatcttcat
    42901 tcaaaatgac attatacttt tgtaataatt gaggaaaaac tattatttct atagcaccat
    42961 acaaatcttc taattcaata aatgccataa tttcatttcg ttttgttgtt ttaatagttt
    43021 tagttacaat cattccaccc attataacat ctgtttcatt catttgtagg tatgactctt
    43081 cttcctcttt taatgaattt aattttccat tatccataga tgttttttct tgtagttctt
    43141 tttcaaattg tgataaagga tgtccactta catacattcc caagacttct ttttcaaggt
    43201 taagtctctc tctttcttca tactcttgcc ttttaggtaa actgtatatt tgttgaaaat
    43261 cactatcttc ctcactatct ccaaaagcat caaataaaga aatttgacct tgaacatttt
    43321 tctttctatc catagataca ctttctaata aactttcata accagccatc agactagctc
    43381 tattttcact tatattatca aaagctccac attttattag actttctatt actctcttat
    43441 ttgtatcttt ttggtctaat cttttgacta aatctactaa atctacaaat aatccattag
    43501 attctctttc agcgattata ttgtttatta tatttacacc aacatttttt acagctgcta
    43561 gaccaaatct tatattgtcg ccttcaactg aaaactttga aaaacttttg tttatatctg
    43621 gaggtaatac atctatttct agtgctttac attctcttat atactcaact accttatcag
    43681 tatttcccat tacactggtt atcaatgctg ccataaattc aactggataa tatgttttta
    43741 gataagctgt ctcatatgct aaaaccccat atgcagctgc atgtgattta ttaaatgcat
    43801 atctagcgaa gtctatcata tcatcaaata ttttattagc tatttcctct tctacaccat
    43861 ttctgataca ccctgcaatt tctatattac catcttcatc aaatttacca tgaataaaat
    43921 attgtctttc ttcttccatc acgtccatct ttttcttact catggctctt ctaactaggt
    43981 cacttcgtcc gtaactataa ccacctaaat ctctaacgac ctgcataacc tgctcttgat
    44041 ataccaaaca accatatgta acttccatta taggctttaa tttttcatgt agatatgtta
    44101 catcttttgg gttgctttta ttttttatat atgcaggtat agaatccatt ggacctggtc
    44161 taaataggga tattcccgct acaatatctt caaaattgtc tggttttagc tgtttcataa
    44221 aacttctcat gccagcactc tccaattgaa acacccctag tgtatttcct gatgaaagca
    44281 tttcatatac tttcgggtcg tcataatcca tctttgaaaa gtctattttt tctgtgtagt
    44341 tttttataac tctatttttt tctattaaat caagagtatc tcttataact gtaagagttc
    44401 ttagcccaag aaaatccatc tttaaaagcc caagttcttc aagtgttgtc atagtaaact
    44461 gtgttgtgat tgcatcttga tgtttgtaca aaggtacata ttcatctacg ggattttttg
    44521 atataacaac tcctgctgca tgagtagaag catgtcttag cattccctct atctgttttg
    44581 atatatttat tatttctttt gtttccgggt cttggtcata caattcttta agattaggat
    44641 ttgtatctag ggccttatct attgtcattc ctaaagcaaa tggtatttcc ttggctacct
    44701 tgtcaacttt attgtagcct atatccaata cccttccaac atctcttatt gcagcttttg
    44761 cacccattgt tccaaaagtt attatctggg ctacatggtc atcaccatat tttctcttta
    44821 cataatctat aacttcttct cttctctcat aacaaaaatc tatatctata tctggcatgg
    44881 atatacgttc tggatttaaa aaacgctcaa ataatagaga gtactttatt gggtctatat
    44941 cagttatctt aagtgtatat gctactatcg aaccagccgc actacctcta cctggtccta
    45001 ctataatatt attttcttta gaaaaattta taaaatccca aacaattaaa aaatactcta
    45061 cataacccat tttttcaatt acatctaatt cataatttaa tctctctagt atctcattgc
    45121 taggattatc atatctctct tttaatccat taaaacacaa ctcccttaaa taagtttctg
    45181 gtgtatatcc ttctggaaca tcatacttag gaagatgtat agtattaaaa tcaaactcca
    45241 cattacacat atcagctatt ttcacagtat tgtcaatagc ctctaatgcg tatggaaata
    45301 attcttccat ttcttctctt gattttaagt aaaactcatc actaccaaat ctcattctat
    45361 ttgggtcatt taatgttttt cccatttgaa tacacataag aacatcatgt attttcgaat
    45421 cttctttatt tacatagtga acatcattag tagcaactag tggtatacca gtctcttttg
    45481 ataattttac taaaccagag tttacttctt tttgctcagg taaattgtga tcttgaatct
    45541 ccaaaaagaa attttcttct ccaaatatgt ctctgtagtc aagagcaaat tgttttgctt
    45601 tttcataatt tctattcatt aaagcacgtg ctacatctcc tgcaaggcat gcagaaagtg
    45661 ctataatccc tttgttatgt tttttaagtt cttccatgtc aactctaggc ttatagtaaa
    45721 aaccttctac gtatgaagtt gaaactattt ttattaaatt tttatatcct tccatattct
    45781 tagctaacag aactaaatgt ccttgatatt tgtcatagtt tgggtcttta tctctcaatc
    45841 cacgtgcagc tgtatagact tcgcacccta taattggctt tattccttct tttatagctg
    45901 ttttgtagaa atctataaca ccaaacatac aaccatggtc agttatagct attgagctca
    45961 tgtttagttc cttagccctt tttataagtt ttttcaccct cgaaaaccca tcaagtaaac
    46021 tatactcagt atgtacatga agatgagcaa aatctttgtt cattaaatca cctcctgctt
    46081 tataatttta attttaccac aaatatatgt cctaaaacaa aaacagagac ttttatctca
    46141 aaataggaat taacttcaac atataccatt atatgttata aattaatgct tattctgaga
    46201 ttgtcccctc aatttattgc tatgattttt ttttcaaatc tgttgcaatt tcttctattt
    46261 ttctaagtct atgattgact ccagattttc ctattggtgg ttctaacatc tctccaagtt
    46321 ctttgagagt catatcctca tactcgattc taatcttagc tatctcttgt aaattttctg
    46381 gcaaactgct aattcctata gtctcttgta tgagcttaat attttctact tgtcttactg
    46441 ctgcatttac tgttttagaa aggttagctg tctcacaatt tactatcctg tttacattat
    46501 ttctcatttc ttttactatt ttagtatttt gaagacttaa aagagcgtgg tgtcctccta
    46561 taatactcaa caaatctgat atttgttcac tctctttgat ataaacaacg tgattatttt
    46621 ttctagccac aattttacta ttaaaaccta aagagtttat aagttctttt agagaatctg
    46681 caaactcctc attattagtt acaaattcca tatgatagtt cttttctggg tcgcttatag
    46741 aacctccacc aagaaaagct cctcttatga aagctctctt acatccttca tgcttaatta
    46801 agttctctga aattttgttt atagtaaaaa agccttcttt atcttcaagt atacctaact
    46861 tttttaataa catttctgaa cccatttcgc ttgttaccat taatacataa ctgttatttc
    46921 tctttagcat ttgattctta tttactgaga ttgtagtatt gatattaaaa ttctttttta
    46981 ataatttaaa tactttacgg gctattgaat ttaactctgt tgttatcttt agatttagct
    47041 tcttatatcc aacgatttgt atacttcccc ctgatttaac tagagctgat aattcagcta
    47101 tattacaaca ttcattccca gatataactc ttgctagttc atttttggtt tcagttgaaa
    47161 aagacatatc tatcacctat taactaattt ttctttaacc ttttttttat ttcttgtaag
    47221 gcaaccttta ttatctcctt ttcatcatca tacctagcta ggtctaccac tctatctaga
    47281 tggataaatt tagcatcaat aaagccttcc tctttttgtg gaatagtatc catattcttt
    47341 gcttgcatca aataccaaaa aactgtttta tgaactgctc tattttcatc ccagttttct
    47401 ttgaatgtat agtgtatttc acctaaatat ttcaatatat ctgcttttac tccagtttct
    47461 tcacttactt cacgtaatgc tgcctcttgg ttattttccc cttcttctac ttttcccttg
    47521 ggtaacaccc aatctccatt gaacttcctt agtaaaagaa tcgtattgcc aaacaggact
    47581 acaccaccag cactaacctc ttctctcatc gatgttcact ccttaaaact aacataatga
    47641 taaggatttc tacacaattt tttaaaatcc ttttttacta acttttgttg taattatgat
    47701 ttagggctaa ctcaataact atgttagata tatactttgc atcatgtctt atataattat
    47761 tttttatttc aattaaattc ttctctacgg tttttatacc catctctttt aatttatctt
    47821 tttgcttttt atctaacaag acttgctctg ctccatcttg tttatataag tcaagcattt
    47881 cttctggcaa tatctcatta tttgcgatta cataatctat taaattctca tcagtgtgct
    47941 ttactatagc attaacatgc tctaatacat tgtacccttc tgtctcacct ggttgagtca
    48001 ttatatttgc tatatatact tttggagcta cagacgattt aatagcgtct acaatcccct
    48061 ctacaagtaa attgggtatt atactcgtat ataagcttcc agggcccata attataatgt
    48121 cagcatcgta aatagatgca ataacatcct ttaatggttt gacatctttt ggatttaaaa
    48181 aaattttatc aatagagctt ttttgaattt ttgactcttc aggtatacta gattctccat
    48241 ttattatatt gccattttct aatttggcaa ctaaatttac atcttccaaa gttactggta
    48301 aaactcttcc tgttatagca aaaatttcac ttaatttata tacagctttt tcaaagtttc
    48361 catacaaccc attcatagca gccaaaaaca aatttccaaa actctgacct tttaaaagcc
    48421 cttctgtaaa cctatattgc ataacttcat tcattgttgg ctctatattt gcaagtgcta
    48481 atagacagtt tcttatgtct cctggtggga gcattcctaa gtcttctctc aatacacctg
    48541 aacctccacc atcatcagca acagttacaa ttgcagttat attttgcgta gtgtgtttaa
    48601 gacctcttaa aaatactgat tgaccagtac ctccacctat cacaaccaca tttggttctc
    48661 tattttcaac aatagacctt tgaaatagtt ttactttttc attttttgct tttttatata
    48721 taaataacga tacaattaat gcaatccaac ctaaaattcc tacaataata agtaatttag
    48781 ccatgtttgc ctccattatc gcaacataga gtctctatgt ttctttacaa ctctatatcc
    48841 cttattagat aaatcctccg ctataagatt tgttatagta actgacctat gtcttccacc
    48901 tgtgcatcca acaccaatta ctagatgttg ttttccttca tctatatatt gaggtactaa
    48961 aaaatgaatc atatctaaaa gtttcacata aaattcttca ctgattttag aattcataac
    49021 ataatctcta acttctttat catcaccagt tttagctctt aactcttcaa cataataggg
    49081 atttggaaga aatcttacat caaatactaa atctgcatca gcaagtatac catgtttaaa
    49141 tccaaaagat actacagaaa tagttaggtt tggattatct tcaccagaag aatatatttt
    49201 ctttatttct tccttaaggt ctttaggttt catgttagtt gtatcaatta tacatgtaga
    49261 taactccttt aaaggttcca ttatctttct ttccattttt ataccttctg gaatctgcat
    49321 atcttttgcc aatggatgat ttcttcttgt cattttatat ctctttaaca atacatcatc
    49381 attacagtcc aaatatacca tttcatattc ataattttct ttttctaaat aatttaagct
    49441 ttcatgaagc gcttcaaaaa actttctccc tcttatatct atccctagag caactttatc
    49501 aatacttgaa tttggttgat aacataattc tgcaaattta gttatcaaag caggaggtag
    49561 attatctaca caataaaatc ccatatcctc taaagctctc attgtctcac tttttccaga
    49621 tcctgaaaga cctgtaacta taacaaattt cattgctaac tcctcccttt tatcttatgt
    49681 ttattatatt ataatgtttt ctactaacga tatatccaga ttagagtcag atatagtctt
    49741 catagctagt ttaaaatcac caactctttc tggacaatgt gcatctgagc ctattataaa
    49801 tttattttct agccctttta ttttatttaa ttgatttaca ttcaaaaaag aatggctact
    49861 atttatttca agtctagtat ttgtcttttt tgctgtcttt gctacttctt ctatatacac
    49921 atcgccttta tctcctggat gagtaattat aaaaatatca ttattactta tcgcatttat
    49981 tacagctttt gtattgtatt cttttgcttt ttcagtttta aaaatatagt tattacaatg
    50041 atttaataag cttcttaatg atgttggagt agaaccaaaa tgatatccag ccataatata
    50101 atcaaaatct ctaataaatt tatcatccat atctatattg cctttatcat ctaatatgtt
    50161 acactccata cctaaaagta tatttatatc attgtactta tcatttaaat aatctacttc
    50221 ttctctcatt ttgagaatat cttttttctt tacaccatat gcaatatgtt tatagctatg
    50281 gtcagatatt cctattgttt ttattccttt agaaatggct acctttacat tatcttcaat
    50341 agtccctttg ccatgactat ataatgtatg tgtatgataa tcagccagta ttttcatttg
    50401 ttaatcctct ccaagtattt tgacttcttc ttccaacata accccaaact tggcatatac
    50461 agcacttttt ataacatata ttaaatccaa aatatcttta gcacttgctt ctccttgatt
    50521 gactacaaat ccacaatgct tctcagatac ttgtgctcct cttaaagtaa gacctctcaa
    50581 tccagtttct tctattaatt tacctgcaaa atatcctgtt ggtctcttaa atgtacttcc
    50641 tgcactcgca aaatttaatg gctgtttagt tattcttctt gtcgctaaat catccattaa
    50701 actttttatt tcatcataat ttccttcttg taattctagt tctgctgata aagctatata
    50761 tccgtttttc gatattatac tttttctata cccaaattcc atctgttcat ttgataattc
    50821 aaatatattg ccttccatat ccatcagtct tactgacttc actatgtcct tcatttcacc
    50881 accatatgct ccagcattca tggctagtgc tcctcccaat gtaccaggta ttcctgatgc
    50941 aaactcaaac ccttttaatt cttgcttttg gagagcttta cctaaaactg ataataaagc
    51001 tcctgactga gcagtcatcc tagtatcatt tatctcataa gaattgaaat tatcagctaa
    51061 ttctattacc actcctctta ttccaccatc tttaattaaa atgtttgaac catttccaat
    51121 tattaaatac ggaatagatt cttcttttac caacttaagt acatttttta attgttcttc
    51181 agttctaggt ctaactaata tatctgctgg tcctcctacc ctaaatgaaa tatgtttttt
    51241 cataggttca tcgactttta tatcctcttt acttaatata tttaataaat ttatatatat
    51301 atcttgatta ttcattgtat ttcctcccaa attatactga actttagctg tcattattat
    51361 ttaaattaca ttagtatata gtattaacta tattcaataa tatatgattt tgcttatcat
    51421 atggatttta tattttaagc ttcttaatta caaactatat tcaataaata tataataaag
    51481 taaaaaaaaa aaaataacaa catataaaag aatataaata atttaaaaaa agcatcaata
    51541 tcactgatat caatgcttta aaaggcaatt taattcgtat tatttatcta actatttttg
    51601 acatagcaaa gataataaat ccctccaact actatggaac ctccaacgat atttccaagc
    51661 gttacaaata ccaaattagt tataaattga accatagtta tatttgctcc taatgccatt
    51721 cccattggta taaaaaacat gtttgcaaca ctatgttgga atccgcataa tacaaataac
    51781 attattactg tccaacatga agccaatttt gacattatat cttgagatgc tgtagccatc
    51841 catacagcta aaacgactaa aatattacaa agtattgctt ttaaaaacat tgatgctata
    51901 gttgaattaa ccttagcttc tgccacagct atagcttttg tagctgcatc tcctgtcaat
    51961 gtagaagaat aaaacacaag tgcaactagc agaattgaac ctactaaatt tgcaaaataa
    52021 accagtcccc agtttcttaa catttttcct aatgttattt ttttgtccat aagtgcaata
    52081 gccattaagg tgtttccagt gaataattct gctcctgcca ccactacaag cataagacct
    52141 actggaaaga ccatagcact taaaaagcta gctacccctg ggtctatctt gccaaaagtt
    52201 tgactaacta tcatactccc caatgcccca aaacctataa atgctcctcc aaataaacct
    52261 aatagaattg cattttgtgt cgaaatgttt gcttttttaa tgcctgcact tatagttgct
    52321 tgtgcagttt ccccaggtgt caagaatcct ttactcataa atccttcccc cttaaaaatt
    52381 atactttccc ttgtcttttt atggcaataa ttatataata acaatatgat atataataaa
    52441 ttttattaac ataaaaaata tactatatac ttatatatac ttgtctcata taattatact
    52501 atttattata taacaacttt gttaatttcg aaataattta tattgaattt gaaaaattta
    52561 ccttttgatt atttatttta ctttttaatt attgaggtat tatatataat agatatatgt
    52621 ttgtgttatt atatataatt acttatttta ataaatattt gaaggaggga attttatgtt
    52681 tgatatatca tttttcgaaa tgtcaatata tgaaatagca gttactacaa tatatataat
    52741 taatttcatg gttatactaa accttatctt tagagaaaaa agaaacataa atacaacttt
    52801 aacatggctt cttatattag tattgattcc tgctcttgga ttcatacttt atatagcttt
    52861 tggcagaaat atttcaaaaa acaatatgtt tagattgaaa gaaaaggatg ataagataat
    52921 caaaagtaat atactagata ctcaggttaa actccaatct acatctgaaa ttgattcaga
    52981 tatacatcaa cataaggata tgatttatgc attggctaat tcaaataatg ctcattatac
    53041 caataataac gatgtgtgga tttatgctga atctagtcaa ttttttaaca gtcttcttga
    53101 agaacttaaa aaagctaaaa aatatataaa catacaattt tacattttca aagatgacaa
    53161 aatcggaact gaaataatag acattcttat agacaaagct aaagaaggtg ttgaagtaag
    53221 acttttattt gatgcagttg gtggtagaac actaaaaaat agtacacttt ccagacttaa
    53281 agaaagtggt gttaaagttg gaagcttctt cccatcattc ttgaaaattg taaactttaa
    53341 cttaaactat agaaatcacc gaaaaattgt tgtcattgat ggtaaggtag gatttgtagg
    53401 tggatataat gttggtgatg aatatttagg tagaaatcca aaatttggtc tatggagaga
    53461 tacacatact aagcttactg gtgattgcgt aattgaccta aatatgagat tcatccttga
    53521 ttggagatat acaactaaag aagatttaga tttagaaaag tactttatag aagaagatac
    53581 aaattctaat catccttctg aaaatattgg gattcagata gtatcaagtg gtccagatat
    53641 atcagaactt gatgagatta aatatggtta ccttaaaatg attcagaaag ctagaaagta
    53701 tatatacata cagagtcctt atttaatact tgactcaact tttatagata ctttaaaaat
    53761 agcttgttta tctggtgttg atgtgagagt tatgattcct tctaaacctg accatccctt
    53821 tgtatactgg gcatcatatt catatgctgg agaactttta aagtttggag ctaaaatcta
    53881 cacttatggt caaaatgcat ttttacatgc taagacaata gttatagatg actctatatg
    53941 ctctatagga acagctaata tggatatcag aagttttgag cttaatttcg aagttaatgc
    54001 atttatgtac tcttcaaaaa aagcattgga acaaagaatt atatttgaaa atgatatttt
    54061 aaattctaag gaa
//
LOCUS       DACTGW010000004        45556 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.004, whole genome shotgun sequence.
ACCESSION   DACTGW010000004 DACTGW010000000
VERSION     DACTGW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45556
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.004"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            47..379
                     /locus_tag="I9573_000177"
     CDS             47..379
                     /locus_tag="I9573_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAT4794823.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            541..726
                     /locus_tag="I9573_000178"
     CDS             541..726
                     /locus_tag="I9573_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794824.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            753..1838
                     /locus_tag="I9573_000179"
     CDS             753..1838
                     /locus_tag="I9573_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAT4794825.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLYNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSTIPKMKEVIGNHA"
     gene            1831..2289
                     /locus_tag="I9573_000180"
     CDS             1831..2289
                     /locus_tag="I9573_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794826.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            2324..3415
                     /locus_tag="I9573_000181"
     CDS             2324..3415
                     /locus_tag="I9573_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT4794827.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKAGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            3420..3644
                     /locus_tag="I9573_000182"
     CDS             3420..3644
                     /locus_tag="I9573_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794828.1"
                     /translation="MENIKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            3654..4217
                     /locus_tag="I9573_000183"
     CDS             3654..4217
                     /locus_tag="I9573_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794829.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            4178..4807
                     /locus_tag="I9573_000184"
     CDS             4178..4807
                     /locus_tag="I9573_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4794830.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            4847..4984
                     /locus_tag="I9573_000185"
     CDS             4847..4984
                     /locus_tag="I9573_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT4794831.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            4984..5736
                     /locus_tag="I9573_000186"
     CDS             4984..5736
                     /locus_tag="I9573_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4794832.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            5749..6234
                     /locus_tag="I9573_000187"
     CDS             5749..6234
                     /locus_tag="I9573_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794833.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYENIMVVDGNSINFIYPVSALQTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            complement(6526..7347)
                     /gene="yidA"
                     /locus_tag="I9573_000188"
     CDS             complement(6526..7347)
                     /gene="yidA"
                     /locus_tag="I9573_000188"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAT4794834.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNEAPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            7839..9533
                     /locus_tag="I9573_000189"
     CDS             7839..9533
                     /locus_tag="I9573_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAT4794835.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGNIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYVRMMRSDYREDMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      9865..9975
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10079..10450
                     /locus_tag="I9573_000190"
     CDS             10079..10450
                     /locus_tag="I9573_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HAT4794836.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQDNKKTPKWTENVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            10546..11493
                     /gene="trxB"
                     /locus_tag="I9573_000191"
     CDS             10546..11493
                     /gene="trxB"
                     /locus_tag="I9573_000191"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAT4794837.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            11578..11895
                     /locus_tag="I9573_000192"
     CDS             11578..11895
                     /locus_tag="I9573_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HAT4794838.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            12164..13450
                     /locus_tag="I9573_000193"
     CDS             12164..13450
                     /locus_tag="I9573_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAT4794839.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            13562..14035
                     /locus_tag="I9573_000194"
     CDS             13562..14035
                     /locus_tag="I9573_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:13694..13696,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HAT4794840.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            14067..15377
                     /gene="grdB"
                     /locus_tag="I9573_000195"
     CDS             14067..15377
                     /gene="grdB"
                     /locus_tag="I9573_000195"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:15114..15116,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HAT4794841.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            15634..17166
                     /locus_tag="I9573_000196"
     CDS             15634..17166
                     /locus_tag="I9573_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002846562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HAT4794842.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNSDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            17177..18322
                     /locus_tag="I9573_000197"
     CDS             17177..18322
                     /locus_tag="I9573_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HAT4794843.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            18632..19711
                     /locus_tag="I9573_000198"
     CDS             18632..19711
                     /locus_tag="I9573_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAT4794844.1"
                     /translation="MSRVKNVVGLLGTKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            19824..20423
                     /locus_tag="I9573_000199"
     CDS             19824..20423
                     /locus_tag="I9573_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794845.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLPPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIAALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(20610..21482)
                     /locus_tag="I9573_000200"
     CDS             complement(20610..21482)
                     /locus_tag="I9573_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT4794846.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            21899..23302
                     /locus_tag="I9573_000201"
     CDS             21899..23302
                     /locus_tag="I9573_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794847.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            23329..24873
                     /locus_tag="I9573_000202"
     CDS             23329..24873
                     /locus_tag="I9573_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HAT4794848.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            24904..26295
                     /locus_tag="I9573_000203"
     CDS             24904..26295
                     /locus_tag="I9573_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAT4794849.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            26488..27984
                     /gene="abfD"
                     /locus_tag="I9573_000204"
     CDS             26488..27984
                     /gene="abfD"
                     /locus_tag="I9573_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HAT4794850.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            28119..28394
                     /locus_tag="I9573_000205"
     CDS             28119..28394
                     /locus_tag="I9573_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794851.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            28418..29725
                     /locus_tag="I9573_000206"
     CDS             28418..29725
                     /locus_tag="I9573_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HAT4794852.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            29745..30863
                     /locus_tag="I9573_000207"
     CDS             29745..30863
                     /locus_tag="I9573_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HAT4794853.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            31150..32070
                     /locus_tag="I9573_000208"
     CDS             31150..32070
                     /locus_tag="I9573_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794854.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            32185..33342
                     /locus_tag="I9573_000209"
     CDS             32185..33342
                     /locus_tag="I9573_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAT4794855.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(33427..34926)
                     /locus_tag="I9573_000210"
     CDS             complement(33427..34926)
                     /locus_tag="I9573_000210"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAT4794856.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            35522..36946
                     /locus_tag="I9573_000211"
     CDS             35522..36946
                     /locus_tag="I9573_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAT4794857.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDALFKRLESK"
     gene            36981..39110
                     /locus_tag="I9573_000212"
     CDS             36981..39110
                     /locus_tag="I9573_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT4794858.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDK"
     gene            39140..39562
                     /locus_tag="I9573_000213"
     CDS             39140..39562
                     /locus_tag="I9573_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT4794859.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            39581..40732
                     /locus_tag="I9573_000214"
     CDS             39581..40732
                     /locus_tag="I9573_000214"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAT4794860.1"
                     /translation="MKKAIQFGAGNIGRGFIGSLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEEILNNIEKSYIFMKNL"
     regulatory      40838..40939
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            41122..41694
                     /locus_tag="I9573_000215"
     CDS             41122..41694
                     /locus_tag="I9573_000215"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAT4794861.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            41758..42408
                     /gene="fsa"
                     /locus_tag="I9573_000216"
     CDS             41758..42408
                     /gene="fsa"
                     /locus_tag="I9573_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAT4794862.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGAFLKL"
     gene            42560..45484
                     /locus_tag="I9573_000217"
     CDS             42560..45484
                     /locus_tag="I9573_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAT4794863.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYGTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT        17939 a         4996 c         8475 g        14146 t
ORIGIN      
        1 tatctgtaaa attttgtaaa atataaaaag tataggggga ataaaaatga aagtagctta
       61 tatttttagt agtactaata cacataaaat tctagacaaa atgataatac cacaacttga
      121 acaaggaact catggagtag acgttctagg gatgtttttc tttatggata ataccttatt
      181 tttatctaaa ggtaatccag taggcgagag actaagtaaa ttacatgaaa aaactgatat
      241 gataataatg gcttgtgacc aatgtgctat agaaagaggt atagagaata atttggttga
      301 tggagctaca attggatgtt tcccaaacct atatgcttgt ttaggcagtg caggtgtgga
      361 ccaagttata acgttataaa ttagtatagg atacaatcca tagaaaaatt tattttctgt
      421 ggattatttt aagtaaaaga taaattgttt aaagtagggg atgaacaaat gtctaaaatt
      481 ttagttgaat ttataaacac ttgaccatct tgtgatgaac atggagtgtt tataaaaaaa
      541 atgtcagaaa agtatgctga taaattggaa ataaaattat atcaagcagg gaaagatttt
      601 tcgtatataa aaaaatatgg gattataaca aaaggaactt taataataaa tcagaaaaag
      661 aaatatgata gattaaataa agatacaatt gaaagggcaa tagtagaagc tataaacaat
      721 aattaaaagt ttaaaaatta ggagtatcaa aaatgaatat attagaaatt gttttaaatg
      781 gagttaaaga tactatagta gcttctatag ttacactgaa tggagcatca ggatggctta
      841 tgataagttt tatattggca ggtgtacttc acaatgtttt aactccaaga aggtttcaaa
      901 aacaacttgg aaataagaaa ttttcttctt taattaaaag tactatctca ggaatgtttt
      961 taccaatatg tagttgtgga gtaataccac ttggaataag tatgtattat tcaggagcat
     1021 atttaggacc agtacttgct tttatgacat caactccaat aataaatcca atagctgtag
     1081 ttctttgtct aggtctttta ggtcctaaag ttacaatcat ttatctaata gctggttttt
     1141 tagtaccatt tttagtagga tttttaggaa attgttttgg aaaagaagaa ttatcacttt
     1201 ataatgattc agaggaagaa gtaatagaat tagaagaaga aaaaagaagt tttatggaaa
     1261 atatgattga tggtcttaaa tggtcttttg gtgaattagc ccttactatt agtaaatatg
     1321 tagtaatggg tatggttttt gcaggattta taacaactat ttttccaaat agtataattc
     1381 aaaaatattt aggaaatcca ggtttgctat ctcttggaag tatagcaata cttgcatgtg
     1441 ttatgtatgt atgtgctgta ggacatatac cgtttatagc agcccttgtt gcaagtggag
     1501 catctcctgg tatagcgata acatttctaa tagctggagc agctacaaat ctaccagaac
     1561 ttataagcat gtttaaactt attggaaaaa gaactgttat tatatattct gtaactttga
     1621 cactgtcctc aataataatt ggatatataa caaatttact tttagtgaat aataattcag
     1681 taaatttaga tgcaacaaat tcttcaatac agttggcaaa taaactaatg ttaaatatac
     1741 ctgatagttt tgagtatgta tgtactattt taattatttt atttgcattt aaatcaacaa
     1801 taccaaagat gaaagaggtc ataggaaatc atgcttaaaa agatagttat aatatcctgt
     1861 atagtagttg tgttaatcat tttatcaaaa atagttgatg ataacatcaa agaagatgct
     1921 tctattccaa atgtgaataa agaaacatta gaatatttta gaaaaaatta taaagaagac
     1981 ataataactt gtgctgaaga agatttaaat aatgatggga aaaaagattt agtagttata
     2041 tataaaaaaa gcaataattc aaatgaaatg gtagtcgtag tttctgataa aaatagccac
     2101 tatataacaa agccaatacc agcacctata gagaatcaaa ctataacctt taaaaatatt
     2161 gatgataaag caccaataga agtaatagta tcagggtcta aaaatggtaa tgtaggatat
     2221 gctatttaca gagtagaggg taaaaaattt gttgatttat ttggcgaaga tatggacaaa
     2281 tgttgctaaa aataaaaata atacataaag gtgggaaatt tatatgaaat tatctaagaa
     2341 gattcaagca ctaatcttat ttggtgtaat gggtacatct atattaacag gatgtagtag
     2401 taaaccaaaa gaaaaagaag aagcaaaagc tggaaaagat gattatacaa taacagtagg
     2461 ttattataat tgtgaccata tgactgcggg accagtagca gaatcggcag gaatatataa
     2521 agatttaggt cttaatgtaa aaactgttgg aaatggtaaa gttcctgaag ctatggcagc
     2581 aggaaaaatg gatgcaggtt acataggaac taaggggtta gttggagcaa tacctaaggg
     2641 ttcaccaata acaatagcag ctaacaacca tactggtggt tctgaatatt taatagtttc
     2701 aaaagatata aaagaaccta aagatttaat aggtaaaaaa attgcaacag atatgagtga
     2761 tttcttatgg acaagtgatt atggtccaga aacaggtcta ccaactgacc catcaaaata
     2821 tgaagttgta aatatggatt cagacaaaga taaatattta gctcttaaaa ctgggaaaat
     2881 tcaagctttt acatcttgtg acccttgggg ttctgtagct gaaaatgacg gtgcaggaaa
     2941 aataatagca tctactcaat ataaagaaaa agctaatggc aaagaatata actgttgttc
     3001 attctcatta aacaaaaact ttataaaaga acatccagat ttagcaaaaa aattggttct
     3061 agcacataca aaagcaatag aatatatata tacaaatcca gctgaagcag ctaaaatatt
     3121 tgcaaaatat tataatgtag aagaagaagt tgcacttaga actatctata agaagactgt
     3181 tggagaaggt agaactctta cttggaaagt tacaggtgaa gaatataaaa ataatttaca
     3241 aatgtataaa gatttaaagg ctttagatga tatacctaaa tatgaagata cgattgatac
     3301 aagcttattg gactcttgtg gagctgatga ttttgacaaa tttataaaag aaaaagttaa
     3361 cccagagttt cctgaaggta tgagttatga agactggaaa gcaaaggtat tataaataaa
     3421 tggagaatat aaaaaatata ataatacttt ttatcattat tttgggaagt atatttataa
     3481 tattttttaa ttttgaagat aaagaagaaa taaaaggaaa attaagaata ggggtatctg
     3541 atgatacctc tggttttgta ataaattata tggtaaatag agactacttt gaaaatataa
     3601 aagtaagtga tataatggat atttttacaa ttaatgattg ctgagcaagt acaatgcagt
     3661 gggctctgag ttcagaggaa atagatatag caatcatatg taaagatgct gcaaaacaat
     3721 atgtaaatgt aaatagtgga tttgaaattg ttggtactgt agttcaaaat tctgatatat
     3781 ttctattaag tgataataat cctgaaaaga taggagtcat tcaaaataga gattaccagt
     3841 cagaattggt taaaaaatac tacaagaatg tggaagtagc tccattatta gggactgcac
     3901 tagcatatgg attagaaagt aatttagtag atggtgttgt aatagattgc ataaagtcaa
     3961 ttgggttaga gggtaaaaga aaatctacaa ctaaattagg agattatgat acctatgtat
     4021 tagttgtaag taaagagttt aaaaaaagta aagcatatag tgattttata acactttaca
     4081 ataaaagtgt tgatgcttta aagaataaag ctacatttaa gaaagcatta aaagagtaca
     4141 ggaatataag aaatatagat atggaggaaa taagcaattg gaaattaaaa tttctaaaga
     4201 taaaagcgat agattaacta agaaaaaaac tgatggtagt atggatattt tatataaaat
     4261 aatactatta gttattatat ttttagtctg gcattttgca gcaaaagata tagggagttc
     4321 tttgttactt ccaatgccag tagatgtaat aaaaggtttc tttttctgtg tgacagatgc
     4381 agagacagtc actaatcttt ttataacatt gcagagagta cttaaaggtt ttatgtatgc
     4441 attattgttt gggcttccaa taggatttat tatgggattt tctaaaactt ttgaaagggt
     4501 tttatctcca gtagtagatt ctgtaaggca agttccaata atggcatggg ttcctcttac
     4561 tatagtatgg tttggtatag gagatggtcc aacaatattt ttaatagctt tttcaggggt
     4621 tttccctata attttaaata ctattcaagg agtaagagca atatcaaagg attattataa
     4681 tgcagctaga agtatgggag catcgccaat tgtgatattt acaaatgtaa ttgttcccgc
     4741 atctcttcca gatatactaa ctggttcaag gattgctata agtacaggat ggatgtctgt
     4801 tatctgagcc gagtttatag ctacgagtgc cggtctcggt tactcaatgg tacaagctca
     4861 aacattaatg catacagatg tattaattgg gcttatgata tttgcagctt taataggatt
     4921 tataatagat agggtattaa agtttataaa taaaatcctt tgtagatgga ggtttgcaga
     4981 ttaatgaagt taagtgtaag agatataaat aagacatttg taaataatag agtacataca
     5041 aaggttttag aagatataag tatggatata gatgatggac aatttgtctg tcttttagga
     5101 ccatcaggtt gtggaaaaac aactttactt actataatag gtgggtttca aaaatcagaa
     5161 cgtggagatg tttttataaa tgataaaaga gttaaaaaac cgggcataga tagagctttt
     5221 atatttcaaa attatgcttt atttccttgg aaaactataa gaggtaatgt tctatttcct
     5281 atgaaacagc agaaaatacc aaaagaaaaa agagaagaaa tgctagaaga acttttggtt
     5341 atgtcagact tgaaaggaaa agaaaaactg tttccgcatc aattatctgg aggaatgaaa
     5401 caaagagtgg ccatgataag agctttagca tgtaatccag aggtattact aatggatgaa
     5461 ccattaggag ctgtagattt tcaaatgaga caaaatttac aggaagagct agaacgaata
     5521 tggataaaga agaaaataac tgcattaatg gtaactcatg atgttgatga agctgtatat
     5581 atgagtgata gagttatagt aatgtcgaga gataaaggaa gaattattga tgatataaat
     5641 atagatattc caagacctag aattagagga agccaaaaat atgaagagta caaaaacaaa
     5701 ttaactgata ctttatcaaa gtgttatgaa gtttaaagag gagtagacat gagtcaaata
     5761 gaaaaataca atagtttgga taatacacaa cagaaaataa gaatctctat tatggatatg
     5821 ataatagata aaggttctag tgttacactt caagaagtga ctgaatatgt aagtaaaaag
     5881 ttgaatatag aaaaagaata tatagaaagg acactacaat attttattta tgaaaatata
     5941 atggttgttg atgggaatag tataaatttt atatatcctg tatcagcatt gcaaacaaat
     6001 cataaagtaa ctttgagaga taatagaagt ttcagcgcaa tgtgtgctat agatgctata
     6061 ggaacctctt gcacattcaa ccaagatata aaaataaact ctatttgtag tgctacaggt
     6121 aaggaaatag aagtaattat aaaaaatgaa aagattgagt atgtgaataa tccaaactta
     6181 agagtgcttc atataaatct tgataagcat ctaaattggg ctgctagttg ctgaaatata
     6241 atgaatttct tctggacgaa gagtgatttt gatgcttgga caaacgaagc gggattatct
     6301 gatgatgaag atatatattg tttagatatt aatgaggcaa tagtagaatc atataaaata
     6361 tttaaactca aacaaaaggt actatcatag atatacatat atgaaaaact aaaaatgacc
     6421 tttagataaa aatgataggt aatatccata ctatataaat tagtatagag tagggatgtg
     6481 aaaagtattt aaatctaaag gtatattttt agttttttta gatgattaga tagccttatt
     6541 aagtatatat ttatttataa cctttgcaac tccattttca ttattagtat cagtaacata
     6601 atctgcaatt tcctttatac tattacgtgc attccccata gccacaccaa gacctgcata
     6661 ctttatcata tgttggtcat tttcttcatc gccaacagca atagcatttt ctataggtat
     6721 atttagattc gtacataatg ttttaagagc agttccttta ttagtagttt tacctaaaaa
     6781 ttctagataa aaaggagcag accttactat agtatattta ttgaataaat ttttaggtag
     6841 ttgctgtatg atttcttcca atctttctgg ttcatcaact atcataattt tacagatttt
     6901 aatagattca tcaattttat ctttttgtat atatttgact tctatattat ttaatttcgc
     6961 ttcatgaagt gtatattttc caggtgcttc atttggtgta attaaatttg tcattgtatt
     7021 tatgtgtatt ttagtcttat attttttaca aacgttatta tatataaaat caaaatcatc
     7081 taaagtcatt tctacatcat gtatgatttt ctcactttta acttcttgaa taattgctcc
     7141 attaaagcat ataacataat caccttcatc tgttaaattt agttgattta gaagttcttt
     7201 tacaccagga agaggtctgc ctgttgttat aactactttt gccccagccc tttttgcctc
     7261 ctgaatggac tcaaagactt caggagtaat ccttttttgt gtatttaata ttgttccatc
     7321 aatgtctagt gcgattaatt tgtacatatc aacctccaat aaattatata ctcaaatttc
     7381 ctgtgaaaac gttgacacag ctagtgttga acaaagtttc agtgggaaat atggtttgtt
     7441 ttgtatacta ttatatcaag agataagtaa tttagaaagt ttaataatat taatttttga
     7501 taaaaatatt acttatgtga gatgagaatg taaaaacttt gtaatcaata attccaaaat
     7561 tcttaattac aaataatggt atcgtttgca ttgttaatat aaataaatgt atattatata
     7621 gttagaagtt tgaataaata gactgatggt aatttgttat attagttagt tttgtgttat
     7681 aaaatattgt aattccatta atcatatgtt attattcaac atgattataa tataaagaat
     7741 atttaatata tataataatt tataaaaaag tattacatag tacatgtaaa tctatattta
     7801 taaatacaat aaagtaaagt tgtaaaggag tgctttaaat gaagttttca gagtttaagt
     7861 atgaaagacc caattataat agtatgaaaa aagagttttt gagttgtgta gaagatataa
     7921 ataattctcg taattataaa gagcaacaaa aaaatataca taagattaat ttattaagaa
     7981 ataaaataga aacgttgtca aatatagctt cgataagata tagtacagac acatttaata
     8041 aattctacaa agaagaaaaa aattattggg atgaatatat gcctttgtat gaagaattaa
     8101 attcacattt ttataatgct attgtaaatt caaaatttaa atatgatttg ataaaagagt
     8161 ttggagaaca atttttcaca atagtagagt attctttaaa aagtttttct aaagaaataa
     8221 taagtgaatt gcaggaagaa aataaattgt gttcagagta tactagactt ttagcttcag
     8281 ctgagataat gtttgatggg aatataagaa atttatctgg tatggggaaa ttcatgtatt
     8341 ctaagaatag aaaaacaaga gaattagcaa acaaagcata ttataatttc tttgaagaaa
     8401 atgaaactaa atttgatgac atatttgata aattggttaa acttagagat aagatatcta
     8461 aaaaactagg ttttgaagat tttgtagagc ttgggtacgt tagaatgatg agaagtgact
     8521 acagagaaga tatgataaaa aatgttagaa aacaagtatt aaaatatgtt gttccaatgg
     8581 ccaatgagct ttatgaaaaa caagctaaaa gaattggtct agaatattta agttacatag
     8641 atgaaggcgt ggaattttta acaggaaatg caagtttaaa gggagattca agatacataa
     8701 ttcaaaatgg taaaaggatg tattctgaac tgtcaaaaga aacaaatgaa ttttttgatt
     8761 ttatgttaga aaatgaactt atggatttag aaacgaaaaa aggaaaagga gctggagggt
     8821 attgtactta tataccagat tataagtcac cattcatttt ttctaatttt aatcaaactg
     8881 ctgatgatat agatgtgtta acacatgagg caggacatgc atttcagttg tatatgtcta
     8941 gatggattga tatgccagaa attaattttc caacgcttga tagttgtgaa atccattcaa
     9001 tgagtatgga atttataaca tggccgtgga tggatttatt ttttaaagaa gatacagata
     9061 agtacaaatt cactcactta tcatcttcaa ttaaatttat accttatgga gtaattgttg
     9121 atgaatttca acactatatt tacaaaaatc ctaatgtaga taagtcaaaa cgaaaagaaa
     9181 tctggagatt tttagagaaa aaatacttac ctcatagaaa atatggtgat aattcttttt
     9241 tagagagagg ttgttggtgg tttaaacaag gacatatatt taaaaatccg ttttattata
     9301 tagattatgt attagctcaa atttgtgcgt tacaattttg gaaaaaaatg attcaagata
     9361 gagatgcagg atggaaagat tatataaata tctgtaaagt tggaggaaca aaatcatttt
     9421 tagatattgt gagtatggga aatttgtatt ctccatttga tgatggctgt attgagtcta
     9481 ttataggtga tgttaaatct tggtttgatg aaataaatga cagcaaatta taaaaaatta
     9541 tgagaatttt attgtataaa agtgaaaaat taaaaaatat ataaaaagtg attatttgtt
     9601 gtgaaaatag tcttgttttt aactaaattg ataaaataaa ctaaaaaaga tttgtactta
     9661 aaaaaaaaag aaaatacaag aaatatgcaa tatcaacatt tggaaaaaaa agtaaaaatg
     9721 tatacaaaat acatataaaa aaactattgc aaattttata gaatgtttat atacttaagt
     9781 caacagagat agaaaaaagc tatttataga aaacaaagga taaattgctt aattttaaat
     9841 tttctgaaaa aaataataat ccggatgaag agtatgggag actgctttat aaaaagcgac
     9901 cgaagggaca agtttaatct tacttaccca aaagattaga tgaaattctc aggtaaaggt
     9961 gactgtactt ggacggggct ctgaaaatga ttttaacagg gaagacaaat tgggagattt
    10021 gtttttcctg ttttttattt atcaagtaat aaataaaagt ttgaaagaga gtaaaaaaat
    10081 gataataatt acgaataatc caaaagtaaa agaagaagtt caaggcagag aagttttgtt
    10141 taaagacaca acttatattg gaatcttaga agcaagtaga gatttgatac atgaaggtta
    10201 tgaactttta tctcatccac tctatgggag tgtaaaacca aatgaaacac cttacaggac
    10261 tgttatacta aagaaaggaa atcgtttgga tataaactct ttaaccttaa ttgaagaagc
    10321 aatcatcact gcaagtaaat ttcaggataa taaaaagact ccaaaatgga cagaaaatgt
    10381 ccaagacgat tttagagtaa ttgattatga tatattttat aatacaatac aaagaatgca
    10441 gtatgaataa aaaaatttgt taaaaagtta ttgaaacagc ctaaatatta aatctaattt
    10501 aatattaaat aaaatgagaa atttaattgg ggaggataag tcaaaatgga aaatgtgtat
    10561 gacttagtaa taattggttc aggaccagct gggcttgcag caggtcttta tggagcaaga
    10621 gcaaagttaa aaactctaat cctagaaaaa gacaagactg gtggacaaat tgtaataaca
    10681 catgaaattg caaattatcc agggtcagtt ccaaatgcaa caggaccaag tttaatagct
    10741 agaatggttg aacagtgtaa agagtttgga gcagaaatgc taagagataa cattgttgat
    10801 actgagctag atggagatat aaaagtttta aaaggggaaa aggcagaata tagagcaaaa
    10861 gcagtaataa taggaacagg agcaacacca agaaagattg gttgtcctgg agaaaaagaa
    10921 cttactggta aaggggtttc atattgtgca acttgtgatg cagatttctt tgaagatttt
    10981 gaagtatttg ttgtaggtgg aggagacagt gcattagaag aagcaatgta tttaactaaa
    11041 tttgctagaa aagtaacaat tgtacataga agacaaggat ttagatgtgc taagagtgtt
    11101 gaagaaaaag caaaagctaa tccaaagata gagtttttat tagatacagt aattgaagaa
    11161 atcaaaggtg atggaatatt agaatcagtt gtatttaaaa ataaagttac tggagaaact
    11221 catgaatact ttgcagatga agaagatgga acaatgggag tatttgtgtt cgttggatta
    11281 gatgctcaaa ctgatttatt caaaggtaaa gttgatatgg atgaaaaagg ttacataata
    11341 actgatgaag atatgagaac taacatacca ggcgtatttg cagcaggaga ttgtagatct
    11401 aagactctaa gacaggttgt aactgcaact aatgatggcg caattgcatc tatagtagct
    11461 gagaaataca ttgatgaaaa atttgggaac tagaatctgt aaattaagaa atagtgtctg
    11521 taaatttaat gaataatatt taaaattaaa ataaaaattt caagggggaa taagaaaatg
    11581 ttagatttag ataaggctac atttgaagaa gaagttttaa atgcagaagg ttttgtattt
    11641 gttgattttt ggagtgaagg ttgtgagcct tgtaaagctt taatgccaga tgttcataaa
    11701 ttagctgaga cttatgggga taaaataaaa ttctgtaaga tggatacaac aaaagctaga
    11761 agattagcta taaaacaaaa agtattaggt cttccaacta tggctatata caaagatgga
    11821 gaaaaagtag acgaagttac taaggatgat gctacagttc ctaatataga aaacatgatt
    11881 aaaaaatatc tttaatatgt tgataaagta ttaacattaa aagaaaaaat ctatttttat
    11941 ctgaagataa gaagaaaaag gtttttatgt ccaactttgt taaaaaaata attttaaata
    12001 aaataatttt tttacaataa atatttttta gtaataaaat aaagatttaa ggcataaaaa
    12061 cacaataata tatctttaaa aaaataagat aaattgtaaa atgatataat tttgagaaaa
    12121 actcaaatgc atatacacaa attaaataac aggaggtgca agtatgcgtc ttgaattagg
    12181 gaaaatcttt ataaaagata ttcaatttgg tgatgtaaca gaagtaaaag atggagtatt
    12241 attcatcaat aagcaagaga tgctacaaga aattggtgga gatgagcata ttaaatctat
    12301 agatatagaa ttagctcgtc caggtgaaag cgtaagaata acacctgtaa aagatgttat
    12361 tgagccaaga gtaaaagttg aaggtaatgg gggaatcttc cctggtatca tgtctaaagt
    12421 tgatactgta ggagaaggaa aaactcatgc tttaaaaggt gtagctgttg taactactgg
    12481 aaaaatagta ggtttccaag aaggtataat agacatgact ggagaaggag ctaagtatac
    12541 tccattttct aaattaaata atgtagtagt aatagctgaa cctattgatg gtttaaaaca
    12601 atacgctcat gaaaaagctg ttagaatgat aggattcaaa gcagcaatgt acttaggaga
    12661 agtagcaaga aatcttactc cagatgaagt ttcagtatat gaaactaaac ctcttcttga
    12721 atcaataaaa gaatatccag agcttccaaa agtaggatat gtttacatgc ttcagactca
    12781 aggtttatta catgatactt atgtatatgg agtagatgca aaacaaatag taccaacttt
    12841 attatatcca acagaattaa tggacggagc aattgtaagt ggtaactgtg tttctgcatg
    12901 tgacaaaaac ccaagttatg ttcatataaa caatggggtt gttgaagact tatatgcacg
    12961 acatggtaaa gatataaact ttgtaggtgt aattataact aatgaaaatg tttacctagc
    13021 agataaagaa agatcttcaa actggacttc taaattatgt aaatatttag gattagatgc
    13081 agttatagtt tcacaagaag gttttggaaa tcctgatact gaccttataa tgaactgtaa
    13141 gaagatagaa atgcaaggtg ttaaaacagt tatagttaca gatgaatatg ctggacgtga
    13201 tggaggttct caatcacttg cagatgcaga tgtaagagct gatgctgttg taacaggtgg
    13261 aaatgccaac caagttgtag ttcttcctaa gttagataaa gttattggac atttagaagt
    13321 agtagatgta atagctggtg gttctgatgg aagtttaaga gctgatggaa ctattgaagt
    13381 tgaaatccaa gctataacag gtgcaactaa tgaaacagga tttggtcatt taactgctaa
    13441 aggatattaa gaagtatttt attaaacgat tgagtaatgt taataaaaca tattatagat
    13501 aattataata agtgttatag atatataaaa atatattaga aatataataa ggaggattat
    13561 tatgagttta cttagtaata aaaaggttct tataataggt gaccgtgatg gtataccagg
    13621 acctgcgata gaagaatgtg taaaaacagt agaaggagca gaggttgttt tctcatctac
    13681 agaatgcttt gtctgaacag ctgctggggc tatggactta gaaaatcaaa acagagttaa
    13741 agatgctgct gataaattcg gagctgaaaa tgttgtgatt ttactaggtg ctgctgaagc
    13801 cgaagctgca ggtcttgcag ccgaaacagt aactgctgga gatccaactt tcgctggacc
    13861 acttgctgga gttgccttag gattaagtgt ttaccacgtt gttgaggaac caataaaatc
    13921 attatttgat gaaagtgtat atgaagacca aataagtatg atggaaatgg ttttagaagt
    13981 tgaagaaata gaagaagaaa tgtctggtat aagagaagaa ttttgtaaat tttaatgcct
    14041 aaaagcaaaa ggggtgaaac taagagatgg gaaaactaaa agcagtacat tatattaatc
    14101 agttctttgc tggtataggt ggagaagaaa aagctgatac taagccacat gtggcagaaa
    14161 ctttacctcc aataagttta caacttgata aattattagg tgaagatatt gaaatagttg
    14221 gaacagtagt ttgtggagat agctatttca atgaaaatat tgatagtgca agtgaagaag
    14281 ttttatcaat ggtaaaaggt tttgaaccac aacttttcat agctggtcct gcatttaatg
    14341 ctggtagata tggggttgct gctggaacaa taactaaagt tgtaaaagat gctttgaata
    14401 taccagcttt aacaggtatg tatgttgaaa atccaggagc agatatgttc aaaaaggatg
    14461 tttatgtagt agaaacttct gattctgcag caggtatgag aaaggcactt cctaagatag
    14521 ctaaacttgc agtaaaactt gctaatggag aagaaattgg aactcctaaa gatgaaggat
    14581 acatagctag aggaataaga gttaactact tccatgaaga cagaggttct aagagagctg
    14641 ttgacatgtt agttaagaaa ataaaaggtg aaccatttga aactgaatac ccaatgccaa
    14701 actttgatag agttgaccca agtaaagctg taaaagattt atcaaaatgt aaaatagctt
    14761 tagttacttc tggtggtata gttccaaaag gaaatccaga tagaatagag tcttcttcag
    14821 cttctaaata tggaacttat tctatagcag gagttatgga tttaacagaa gagacttatg
    14881 aaactgcaca tggtggatat gacccagtgt atgcaaattt agatgcagat agagtattac
    14941 ctgttgatgt tcttagagat ttagaaaaag aaggtgtaat tggaaaatta catgagactt
    15001 tctatacaac tgtaggtaat ggtacatctg ttgcaaactc taaaaaatat gcttctgaaa
    15061 ttggagcagc attagtggct gatggtgtag atgcagtaat attaacttct acatgaggta
    15121 cttgtactcg ttgcggtgca acgatggtaa aagaaataga aaaaactgga cttccagtag
    15181 ttcatatgtg tacagtagta cctatttcat taacagtagg ggctaataga atagttccaa
    15241 ctatagcaat acctcatcca cttggaaatc cagcgcttga ccctacagaa gaaaaagccc
    15301 taagaagagg ccttgtagaa aaagcattaa atgctttaac tacagaagta gatggtcaaa
    15361 cagtatttga aaaataaaaa taacacacac atatattata tagataaaaa tataaacaca
    15421 cactaaaaat aaaaaataac tatacacaaa aaaaataata tagatttcat gaatggataa
    15481 attggagtta ttcattcatg aaatcttaaa aaatgccaaa atacataaac aataatagaa
    15541 tcaaatatac aataaaatac aatgattaga gcttttaata agtttaataa gattaaaaaa
    15601 taagtgttca taaaatttat ggaggtgcac aaaatgactt atccagtttt aaaaggggca
    15661 ggctatgtac ttattcatac tccagatatg atagtgcaaa atggaagtac ttgtacagtt
    15721 gaaagagcaa caaattcaga ttcagaattt ttaaaagaag taagtaatca tataagaagt
    15781 tatgaagatg tagttaacta catgccaaat caagtttata taggaaatag aagaccagaa
    15841 gaattaagag atttaccaat gccatggtgt gaacaaaaga ttgagggaac tagaaatggt
    15901 aagtttggag aaataatgcc tcaagatgag ttcatagctt taatgcaaat aagtgatgca
    15961 tttgatttag taaaattatc acaagaattt attgatgaag taaagccaaa aatcgaaaat
    16021 aactaccctg aaattgcacc atttgtaggt aaattaaagg gtgatgatat agaagaaggt
    16081 aaagaattag tagcaacaca catagctgaa ggattatacc atgatggaaa atttgttggt
    16141 tatgtaaaaa gagcacatga tgtagatgtt aacttaaatg cccatacaat gtttgaaaat
    16201 ctagtagtaa aagcatctgg agttttatct gctattcaaa tgctaagaca tagcaaaata
    16261 gaccctgctg aaatagatta tgttattgag tgttcagaag aagcttgtgg agatataaac
    16321 caaagaggtg gaggaaactt tgctaaatca atagctgaga tagcaggact tcagaatgca
    16381 actggttctg acacaagagg attttgtgca gcaccaactc atgcattaat tcaagcagca
    16441 gctttagtaa aagctggcat acataaaaat gtaatggtag tagcaggtgg agcaagtgct
    16501 aaacttggaa tgaatgctaa agaccatgtt aaaaaaggtc ttcctgtact tgaagatgtt
    16561 gttggaggat ttgcagtact tgtatctgaa aatgatggtg taaatccagt tataagaact
    16621 gatttaacag gtaagcatac tgttggaact ggttcttcac cacaagcagt tatgacagca
    16681 cttataactt caggtcttga tagagctaat ttaaaaataa cagatgttga tgtgtattca
    16741 gttgaaatgc aaaacccaga tataactaag ccagcaggag caggagatgt tccagaagca
    16801 aactacaaaa tgataggggc acttgctgtt aaacgtggag acttagaaaa gaaagaattg
    16861 aaagattttg tttctaacaa aggattacca ggatgggcac caactcaagg acatatacca
    16921 tcaggagctc cttatatagg atttttaata gatgatttaa ctacaggaaa tagaaataga
    16981 gctatgatag ttggaaaagg tagtttattc ttaggaagaa tgactaactt atttgatgga
    17041 gtatctttta tagctgaaag aaatacaggt gttactgaag agacttcagg aatatctaag
    17101 gatgaaatta agaagataat agctgaatct atgaaaaaac tagcattaga tatgttagaa
    17161 gaataggggt gaggatatgt caaaaaaagt aatcgcagat gtattcttag aagtagctaa
    17221 tgcaatagaa agcggcgaat ttggcaaaaa agtaaaaatt ggtgttacta cacttggaag
    17281 tgaacatggt gtagaaaaca tggttaatgg tgcacaatta gcaaaatcta atttatttga
    17341 tattgtgctt attggaccaa aagtggaaac tgaccttgaa gtagttgaag ttaatgatga
    17401 aaaagaaatg catgcaaaaa tggaagaatt attagattca ggatatattg atgcttgtgt
    17461 tactatgcat tataattttc caataggtgt atctacagta ggtagagtaa taactccagc
    17521 aaaaggaaaa gaaatgatac ttgcaacaac aacaggaaca agtgctacta atagaataga
    17581 ggcaatggta agaaatgcca tttatggtat tgctacagct aaatcaatgg gtaacaaatg
    17641 cccaaaagtt ggaatactta atgttgatgg agcaagacaa gttgagaagt gcttaaaaga
    17701 attaaaagat aatggatatg acatggagtt tgcggattca attagagcag atggtggatg
    17761 tgtaatgaga ggtaatgatt tacttgtagg tgcacctgat gttatggtta ctgatacatt
    17821 atctggaaat atatttatga aagtattttc ttcatatact acaggtggag attatgaagc
    17881 tcaaggcttt ggatacggac ctggagttgg agaagactat gatagaaagg tacttatagt
    17941 atcaagagca tcaggttctc cggtagtagc aaatgcttta aaatacgctt atgatgttgt
    18001 aaagggagat ataagtaatg ttgcaagaaa tgaatttgca aaggttaaaa aagcaaaatt
    18061 tgatgacatt atatcctctc ttactaaaaa agaagttaaa gctgaaaaag tagaagttaa
    18121 aatgccagat aaagaaattg ttactcgtca aatagctggt gttgatataa tggatttaga
    18181 agatgcagta tctgagttat ggaaaaacgg aatctatgct gaaagtggta tgggttgtac
    18241 aggtccaata gttttagtaa atgaagctaa aggagactta gcagttgaaa ctttagttaa
    18301 agctggatat actgcaaaat aagtacattt tttatatgta aataagaaca tatagttata
    18361 attttattga ttataattat atgttctttt tattttatat cttgtcaata tttatatatt
    18421 aacacaatcc atttatttga ttctctagct aatttattat atttattcaa ttaaaattaa
    18481 gctttctaac taaataaaaa ctaatgaagt attaaaatga aattaataaa atatgaaaaa
    18541 attttaggaa taaagtatct gttagataaa aataagatta attagtgata taatataatt
    18601 aagataaaat ttattatttg gaggtaaaat catgagcaga gtaaaaaatg tagtaggatt
    18661 gttgggaact aaaggtgttg atgcactata tctaacaaag aaaactaatg taaactatat
    18721 aagtggtttt cctgatgaag aagcatatgc agtgatttgc aaagatggaa actttttagt
    18781 tactgatagt agatatatgg agcttgcaga aaaggtttgc aaagattttg aaattataaa
    18841 ttggcataat tttgatagaa gtgtggcaaa ggcagttaaa agtgtatgtg ataaagtggg
    18901 tataaagaaa cttgggtttg aaagaactaa tatagtgttt gataaatatg aagaacttaa
    18961 aaatctgatt gagaaggata atggagaatt aatacctaca gaaaatattg tagagacttt
    19021 gagatatgta aaagacaaag atgaaatcaa aaatacaaga aaagcttgtg aaatagcgga
    19081 taaggcatta gaagaattga ttccacatat aaaagctgga gttagcgaaa ttgaactggc
    19141 tacaaagtta gagtacttta tgaaaatgaa cggagcacaa aatatagggt ttgaaactat
    19201 actgatatca ggagcaaaga cttcattgct tcatggtaag cctagtgata agattataga
    19261 aaaaggtgat tttgtactta tagactatgg tgctatgtat aatggctata tatctgatac
    19321 aactagaact tttatagttg gaggagcttc tgaaaagcaa ttagaaatat ataatttagt
    19381 taaggaagct caaaatgtag gggttgagaa catgaaagct ggtgtacatg cgacaattcc
    19441 agatgctgag ataagaaaag ttgtcaaaaa atatgaagac tattactatc aaggtatagg
    19501 acatggagtc ggtagggatg ttcatgaaga acctttcatt ggtaattatg gtgataaaat
    19561 catagaagag ggatgtataa taacaatgga accaggtatt tatttccctg gatggggtgg
    19621 agttagaata gaagatactg ttttgattac taaaaatggt ccagaaagac ttactaaatt
    19681 ccctaaagat ttgatgattt tagacaaata atttaattta tatatatttt tttacataaa
    19741 atataacata atctcactat taagctctat ttacatatta tagtaaagag ggcttaattt
    19801 tattgtaaga ggtgatagta tatatgtata aaaaacataa atttataaaa aatataaaaa
    19861 aagcctggta taaaatcaaa ttaattgata gatgccttat aattgtaatg gcaatattaa
    19921 tgtttcaatc tatatataat ttatttgtta atgaagtaaa ttcacaagat actacaacta
    19981 ttgatgtagt agtaagaacc acttcagcag ctatatttgg gtatttttta agtgctaatt
    20041 ttataaagag acctttaagg aagtatacaa atactgatgt atcttcaaat ctttttatag
    20101 ataataattt acccccaaaa ggtcaagagt ctagtcaaaa taatataatg aatgtcaaaa
    20161 ataccattgg atttacttca gaagctgata attatgaaaa gaaaatacct attaataata
    20221 atgaaggttt tgaagaagga gaaactagtg aattgcaaat tattatagct actgtaattt
    20281 gtatagcagc attaacagta ctttttgttg taagaaattt tacaactaca acatcagctt
    20341 ctttaggtac tatttcacag atgagagatt ttgtctcagg atgcgtgggt tttttgctag
    20401 gttgtccaag taaacctgat taaaaaagta ttatcaataa aaggtatttt aaaaagtatc
    20461 agtataataa tatttagatt taaatgtata caattataat tctataacat tctatattgt
    20521 atttttatac tgatactcta aactatacta tttattaaaa tattttaaca ttgtatttaa
    20581 acaaaatatt atatctgtat aatataaatt tagtttatat gaatagatgc catatactca
    20641 ataaaaagtt tcatactatg agatatgtat tgatttcttc tccaaagaac tccaatattt
    20701 gcagtaattg cacgttctaa tggtattgaa cagagatttt ctgtattttg aaatatttct
    20761 ttatacaata ttgttgaagc caagtcattt tctagtaaat aacgtatggt agaaagttga
    20821 tttgagtgta aaattatatt tggcttagct ttttcaagtc tttttgtaat aatcttatag
    20881 tgataagaac cttcagataa gattacaaaa ggtacatttt ttaacatata atttgatata
    20941 atgggatgtt ttgccaattc atttttggga tttgtaacta agtacaattc tgttttatag
    21001 attgtctttg aatctatatc atcataaata tcactgtcaa ttataccaat acaaaaatca
    21061 agtgtagatt cttcaatcat tttagctcca acaaatgttg gaacttcaaa aattttaagc
    21121 tctatatctg gataaattac attaaatcta ggaagtattt ttgaaagcaa aaacgtacct
    21181 aaaactgtag gaatacctat tttgagagaa gcttttctct tttttcctaa atcaagtgcg
    21241 tcacattgaa aatcctcaaa ttgttttata aaatgttttg acttgtctaa aaaaattttt
    21301 ccttcaggcg ttaattctat tttattacct attctgtaaa aaagttcaaa gccaagttct
    21361 ttttctaatt ctttaattgc taatgttatg gatggttgag aaatatgaat actttccgca
    21421 gccttagtca tgtttttata tttacagaca gtttgaaaat agtataaata gtttatattc
    21481 atttggtatt agttcctttc atttacatat tagtgacaag tatataatta acaatagcat
    21541 aagttttgta tattgttgta aaaaaataga atagttttaa actatcatag cataagtttt
    21601 tgatattttc aatctattaa gaatagtgct attattaaat taacaaaaga gaatagctgg
    21661 tatggtttag agtaaatcaa tctatattac agttatatca acgaatatca aaaaattatc
    21721 tattagaaaa taaaaattat ttttacaatt aaattgttaa ttaaatcata aagaaaaaag
    21781 tgatattaac aagataaaat ttttttaatc agaaatgtta atttaataac aaaatagagt
    21841 attggggaga aaggagaaat atatttttta ataatatttg ttaatttaat aacgaaaaat
    21901 gaaagggagt gatttagtga agtttgaatt gatggatttt ttaatgaatc cttttgtcct
    21961 tatgtttgct gcagtaatta caggaatttt atttggaaaa attaaatttg ggaagtttaa
    22021 ttttggggta tcaggagcat tatttacagg attatttatt ggatggcttg catatagctt
    22081 ggggaatcta ataatagcaa aaggagaaac agcagcagga tataaggcag caactgtaat
    22141 gatggggaat ggaattattt cttctgactt ctttgatttc tttttaataa tatttgttgc
    22201 agcagttggt ctattggcag caaaagatat gaaagctgta ttaaagaagt atggagcaag
    22261 atttgtaatt ttaggagttc ttataacttt tataggtggc tctatgactt atgcaatgac
    22321 tttactatca agtgataaag gaagtagtgc ttatgaagtt tctggcgttt acacaggagc
    22381 attaacaagt tcgcctggat tagctgctgc tttagaaaca gctggtaaac atgcagaaga
    22441 tgtatcaaaa gaatttgaaa aagcttcaat aaaagataaa aaagaaattt taaaagtagt
    22501 cgacccagaa ggcaaattgg atgtgaatac aactacatca ctgactcaag agcaaataga
    22561 taagtatatt gcatatgctg aagctggtgt tggtataggt catgcagtcg catatccttt
    22621 tggagtttta atagtaatct taggtgtaaa tttccttcca aaattattta ggatggacct
    22681 aaaagaagaa agaagaaaat atgagaagga aatgaaggaa gcaagagata gtgtttctgg
    22741 taaaacgata ccagaagttc catttaatat aataacattt ttcttgacat gtttagctgg
    22801 atacttagtt ggaggaatac atgtatttat gggaccacta gggtacttca cactaggcgc
    22861 aacaggaggt tcactaattg tatctctagt tcttggatat ataggtaaga taggggtagt
    22921 aaatttcaga atggaagaaa aggttttaaa tattttaaaa caaatagggc tagtattttt
    22981 cttagcaata gttggattaa gatatggtgg aaaagtagtt gattcaatta tgacatctgg
    23041 tatgcaccta gccttagtag caatagcagt aggtgttaca gctatgatga taggattctt
    23101 agttggcaaa tacgtattta aattaaattg gattttatta tctggagctg tctgtggtgg
    23161 tatgacatca acaccaggtc ttggagctgc tgttgatgct cttgatagtg atgacccagc
    23221 agcaggttat ggtgctacgt atccatttgc attattaact aaagttatat tggttatagt
    23281 tttacacaaa cttccaatgt aaataagatt ttatatgggg ggatatttat gaataatgaa
    23341 ggagcatttg aagaattaag tggaagatta agaaataaag aattgtgtga tttaataatg
    23401 aatgcagatg aagctgctaa aattatagag gatggcatgg ttattggagt tagtggattt
    23461 acaccttcag gctatccaaa agcagtacca ttagcagttt cagaaagagc taaaagtgga
    23521 gaaaagataa aattaaccgt atattcagga gcatctttag gcccagaagt tgatggagca
    23581 tggtcagaag caggtataat tgaaagaaga cttccttatc aaactaattc aatacttaga
    23641 aataatatta ataaaggtgt tgttgattat atagatatgc acctaagtca ttctactcaa
    23701 tttttaaatt atggaactat acctaaagta gatgtagcta tagtagaagc acttgcaata
    23761 actgaagaag ggaatattat accaactagt gggataggta attctccatc ttttataaaa
    23821 agtgcagata aagtaatagt agagataaat cttgcaaaac caatggaaat ggaaggtatg
    23881 gcagatatat atattacgga aaatccacca aatagaaaac caatagaaat aaatcatcct
    23941 aaggatagaa ttggaacaac atatatacct tgtggtttgg ataaaattgc aggagtagta
    24001 attaccaata tgcaagataa gacccgtcca ttgggggttg tagatgaagc ttcgaaaaaa
    24061 atatcaaata atattattgc ttttttaaga gaagaagtaa aggcaggcag attatcaaag
    24121 aatttacttc ctcttcaatc tggtgtagga agtgtagcaa atgcagtatt atatggatta
    24181 tgtgaatctg aatttgaaaa tttaacttgt tatacagagg ttgtacagga ctctatgttg
    24241 gatttaataa gaatgggtaa agttacaatg gcatctacta catctgtaag tccatctcca
    24301 gaaggattaa taaaatttga aaaagatatt gattttttta aggataagat tatattaaga
    24361 cctcaagaaa ttagtaacaa tccagaaata gccagaagaa ttggtgtaat tgcaatgaac
    24421 actgcaattg aagttgatat ttatggaaat gtaaattcta ctcacataat ggggtcaaag
    24481 atgatgaatg gaattggagg ttctggtgat tttgctagaa atggagcaat aactatattt
    24541 agtactgagt cgattgcaaa aaatggtgat atatcatcaa tagtacctat ggtatctcac
    24601 gtagaccaca ctgaacatga tgtaatggta attgtaacag aacaaggata tgctgattta
    24661 agaggtcttg cacctagaga aagagctata aaaataatag aaaattgtgc ccatccagat
    24721 tataaagaac aacttaggga ttatttaaat agagcttgta aaagtggagc aaaacaaaca
    24781 cctcacatat tggacgaagc tttatcatgg catagtaaat ttatgagtac aggtacaatg
    24841 aaaaaggctg aaacttttaa aagtgcatta taaaatattt aataaattgg aggtagattt
    24901 taaatggaaa aagcagtgga aaactttgaa gatttaagta aggaatacat taatggttat
    24961 attgagagag caagaaaagc tcaaagagag tttgaatgtt atactcaaga acaagtagat
    25021 aaaatagtaa aaatagttgg aaaagtagtg tattataatg ctgaatattt agctaaactt
    25081 gctgtagaag agactggaat gggagtatat gaagataagg tagctaaaaa taaaagcaaa
    25141 gcaaaagtta tatataacaa tttaaaagat aaaaaatcag ttggtataat tgatatagat
    25201 agagaaactg gaataacaaa ggtagctaaa ccagttggag tagtagcggc aataactcct
    25261 tgtacaaatc caatcgttac acctatgagc aatgcaatgt ttgcacttaa gggaagaaat
    25321 gcaattatta tcacacctca tcataaagca attggatgta gtacaaaaac tgtagagatg
    25381 attaatgaag aattagaaaa aataggagct ccagaaaatt taatacaaat acttgaccaa
    25441 caatcaagag aaaatacaag aaatttaatt tcatcagcag atgtagtaat tgccactgga
    25501 ggaatgggaa tggtaaaagc tgcgtactca agtggaaaac cagcacttgg tgtaggggca
    25561 ggaaatgttc aatgtatcat agatagagat gtagacatta aagaagcagt acctaaaatc
    25621 atagcaggaa gaatttttga taatggtatc atttgttcag gtgaacagtc agtaatagta
    25681 gcagaagaaa tgtttgataa aataatggat gaatttaaaa ataacaaagg ttttatagtt
    25741 agagataaag tacaaaaaga agctttcaga aatgctatgt ttgtaaataa atctatgaat
    25801 aaagatgcag taggtcaatc agttcataca attgcaaaaa ttgcaggtgt tgagatacca
    25861 gaagatacaa aaataatagt aatagaagct gatggaccag gagaagagga tattattgca
    25921 aaagaaaaaa tgtgtccagt tatatctgca tacaagtata aaagttttga agaaggtgta
    25981 gctattgcaa aagcaaattt aaatgtagaa ggaaaaggac atagtgtatc tatacattca
    26041 aatacagtta aaaatataga atatgcagga gaaaatatag aagtatcaag atttgttatt
    26101 aatcaatgtt gtgctactag tgctggagga agtttcttta atggtcttgc acctactaat
    26161 actctaggtt gtggttcttg gggaaataac agcatctctg aaaatcttga ttataagcat
    26221 ctaattaata tttctagaat tgcttattat atgccagaaa atgaagtccc tacagatgaa
    26281 gaactttggg gataaaactt agacaaaaaa tattcattct aaattaaagt aggaatatat
    26341 tttaatttag aatgaattta agacataaat taaatttaaa ttagtttatt ggactaaatt
    26401 taaaatatta agagacatat agaagaagga agtattataa tttaaaataa ataaaaaaat
    26461 ctataaaaga gaaggagtgg ttttattatg gcattaatga caggagctca atatattgaa
    26521 agtttgagaa aattaaatac taaggtttat atgtttggtg aagaggttaa gaattgggtt
    26581 gaccatccta tgattagacc atctataaat tgtgtagcag caacttatga cttagctcat
    26641 gacccagaat atgcagattt aatgacagtt acttcaaata tcacaggtga aaaaataaat
    26701 agatttggtc atcttcatca aagtgttgat gatttgataa aaaaagtaaa aatgcaaaga
    26761 ctatgtggtc aaaaaacagc ttcttgtttc caaagatgtg ttggtatgga tgcgtttaat
    26821 gcagtgtatt caacaacatt tgagtgtgat aaagcccatg gaactaatta tcatgataat
    26881 tttgtaaaat acttaactta tatacaagaa aacgatttag tagttgatgg agctatgact
    26941 gaccctaagg gagatagaag tctatcacct agtgctcaac ctgacccaga tatgttcctt
    27001 catatagtag aaagaagaga agatgggata attgtaagag gagcaaaggc acatcaaact
    27061 ggttcaataa attcacatga gcaccttata atgcctacta taagtatgac tgaagcagat
    27121 aaagactacg ctgtatcatt tgcagttcct tcagatgctg aaggtgtgtt tatgatttat
    27181 ggaagacaat cttgtgacac cagaaaatta gaagaaggtg cagatgttga tttaggaaat
    27241 aaggagtttg gaggtcaaga agctttagta gtatttgata atgtatttgt tccaaatgat
    27301 agaattttct tatgtggaga atgggatttc tctggtatgt tagtagagag atttgcagga
    27361 taccatagac aaagttatgg tggatgtaaa gtaggagtag gtgatgtaat aataggagca
    27421 gcagctttag cagctgatta taatggagca aacaaagctt cacatataaa agataaacta
    27481 atagaaatga ctcaccttaa tgaaagctta tattgctgtg gtatagcatg ttcatcagaa
    27541 ggtcacaaaa cagaagctgg aaactatcaa atagatttac ttcttgcaaa tgtgtgtaaa
    27601 caaaatgtaa ctagatttcc ttatgaaata gtaagacttg cagaagatat agctggaggg
    27661 cttatggtta ctatgccttc agaaaaagac tttaaatcag accttaaagt tggaacaagt
    27721 ggaatgacta ttggcgaagt atgtaacaag tattttaaag cttcttcagt ggcttcaaca
    27781 gaagaaagaa tgagaatatt aagattctta gaaaatatat gtcttggttc atctgctgtt
    27841 ggttatagaa ctgagtcaat gcatggggca ggttcaccac aagctcaaag aataatgatt
    27901 tcaagacaag gaaacataaa tcaaaagaaa gaattagcta agaaaattgc gggaataaaa
    27961 aaagaggaag ctttaaattt ataaaaagtt gatatataaa gggaaattat atattaatgt
    28021 gtattaatta atgaaaagat atgtagtata agagatagtt ataaaattat ggtggttttt
    28081 agctaagtaa agaaattttt aataaggaga atttacacat gacctgtgga gttagatttt
    28141 gtgggggctg taaccctaga tacgaaagag ggaaggctct tacaataatc aaagagcact
    28201 tcaatggtag ggtagatttt agcatagcac aagagaacgt cgagtatgat ttgctactta
    28261 taattggagg atgcgcaact ctatgcgcat cctatcaaca atataaagtg aaaaatgaat
    28321 gtgttaagat gtgggatttt agccatatag aaagagttat aaatgtgatt gaatcagcaa
    28381 tttgtgaaac ttaaaaaagc taaatggggg gatttcattg agttggcaag aattgtatca
    28441 aagtaaatta tgtagtgcta cagaagctgt aaaacagatt aaaaacggtg atactgtagt
    28501 atttgcacat tgtgtaggtg aacctcctgc actagtagag gctatgattg aaaatgctga
    28561 acaatataaa gatgttgaga ttaaacatat ggtaagcttg ggtagtggtg gatatactgc
    28621 taagggaatg gaagctcatt ttagagtaaa tccaatgttt gtaagtggaa atgtaagaaa
    28681 ggcaattgaa aatggagatg gtgattttac acctgcattc ttccatgaag taccaaagtt
    28741 gcttagagaa aaaagattaa aatgtgatgt tgttttagca caggtaactc caccagatga
    28801 acatgggtat tgtagcttag gaacatcagt tgattatact tatgaagcca ttaaaactgc
    28861 tagaacagta attgttcaag tgaatgacca gtttcctaga acctatggtg aggtagtcca
    28921 tgtatcagag tttgactata tagtggaaaa atcacaacct ttatttgaac tacaacctgc
    28981 aaagattgga gaagtagaag aagcaattgg taaaaattgt gcttcattaa ttgaagatgg
    29041 ttctacttta cagctaggta ttggtgggat accagatgca gttatgttat ttttaactga
    29101 taaaaaggat ttagggattc attcagaaat gatttcagat gggactttgg ccctttatga
    29161 aaaaggtgtt ataaatggta aatataaaaa ttttgataaa gaaaaaatga cagttacctt
    29221 cttaatgggt actaaaaaat tatatgactt tgcaaataat aaccctgcag tagaggtaaa
    29281 accagtagac tatgtgaatc atccagcaat tataatgaaa caacataaga tggtttctat
    29341 aaattctgct atacaagttg atttaatggg acaggtagtt gcagaggcta tgggacttag
    29401 acaatttagt ggtgttggag gtcaagttga ctttatacgt ggagtgtcta tgggggaaga
    29461 tggaaaggca attatagcta tgccttcaat aactacaaaa aaagatggta cagtaatttc
    29521 aaaaatcgta tctattgtgg atgaaggtgc acctataacg acatcaagaa atgatgttga
    29581 ctatattgta acagaatacg gtatagcaga attaaaagga aaatctctta gagaaagagc
    29641 aagaaattta ataaatatag ctcatccaag tgtaagagaa agtctagcag tagaatttga
    29701 aaagagattt aaggagaaat attaataata gaggggtgtt aataatggaa gcacttagag
    29761 tagttccaaa gatattttat tttgatacat ttaaagaatt taatgaagaa tttaaaatag
    29821 gaaaaaatga tttagtaatt actaatgagt ttatatatga gccatatatg aaacctcttg
    29881 gaatagatac aaatttaatt tttcaagaaa agtttggaac aggtgaacca tctgatgaaa
    29941 tgatagactc catgacaaag gaaatgaaga aatacaattt tgatagaatt attgcgtttg
    30001 gaggaggaac aatagttgat atatgtaaga tattagcact tgatgttcca gaaaagtcta
    30061 ttgatttatt tgaaggagaa gtatcaccta aaaaagtaaa agagttagta gtagttccta
    30121 caacatgtgg tacaggtagt gaagtaacaa atgtagctat tgcagagtta aaatctaaac
    30181 atactaaaaa ggggcttgca gtagaagaaa catatgcaga ctatgcagtg ttaattccag
    30241 agacagtaaa agggttacct tacaaattct ttgttacaag ttcggtagat gctcttatac
    30301 atgctattga gtcttatctt tctccaaaag caagtccatt tacagagatg tattctctac
    30361 aagcaataaa aatgattatg gatgggtata agaagatagt tgataaagga gaagaagaga
    30421 gatttaatca tcttagagac tttgtacttg catcaaatta tgcaggaatt gcttttggaa
    30481 atgctggttg tgcagcagtt catgcactat cttattcaat aggtggagct ttccatgttg
    30541 cacatggtga agcaaattat cagttcttta ctgaagtatt taagatgtat tctagaaaaa
    30601 aacctaatgg aaaaattata aaatgtacaa aaattttagc agatgccctt gaatgtgacc
    30661 ctaactgtga tgtttatgga gaattagaaa agtttttaaa taaattaata gctaaaaaag
    30721 ctttgagaga atatggaatg gtggaaagtc agatagatga atttacagat agtacaatag
    30781 caaatcaaca acgactactt gctaataatt atgtagagct tagtagagag gaaataagag
    30841 agatatttgc aaatctttat taatacaatt ttttatttat atgtatagtc aacataaaca
    30901 atgctaatta tatttatgta caaataaaaa taagggaact taaattttaa ttaaagttaa
    30961 gttctcttat ttttatatgg agttaaatat aagtatattt ttaatttaaa atatgcttat
    31021 atttaagtct attactttat ttgaaacaat ttgtagaatt tcaaattcct tgacagtaat
    31081 aagtaagtaa ttgtataatc aaactgcaat atattaataa tattagtgaa aatgaaagga
    31141 ttataaaata tgtataaatc agaaaatttt tcaaaaaaat ctgtattaat aaattcattt
    31201 ttaagtaaat ttttaaaaat aattggatgg ggtttggtaa gcttatttac attatcagcc
    31261 ttagtacttt caactatttc cattataatt ggatgggctg taatacttcc tattcaagta
    31321 tgtgcagtat ttttacttat aggaggagtt tgtattttta aaggtaagag attaggagac
    31381 caaatttctc gttataaaaa atattgtaca attataaaca atagaaatat tattccagta
    31441 gaacttattg cagaaaacac atctaaatca ttaaatttta taataaaaga tgtacaaaag
    31501 atgatagaca aaaactactt tataaatact tatattgata aaagaaataa tcagattgtg
    31561 ttaacaaatg aagattttat tccaccaaga tatgaagatg tagcatatga agtcaaagaa
    31621 gaagaaaaga ctgatgaatc caatgaagtt gatacaatta taaataaagg aatgaattat
    31681 ctaacacaaa taaaagaagc taataaaaat attaaatcag aatcaatggg cgataaaatt
    31741 gtacaagtag aagatgttac aagtaaaata tttgatgttg taaaacatga cccatcaaaa
    31801 cttactcaaa tacaaaaatt tatggattat tatcttccaa ccactttaaa actattaaat
    31861 tcatatcata cattggaaga acaaggaatt gatagagaaa atataacaac aactatggaa
    31921 agtattgaaa atacaatgaa tacgattgta gtagcttttg aaaatcaatt ggactatctg
    31981 tttgaagatg aagccattga tatttctaca gatattactg ttttagagaa tatgttagtc
    32041 caagaaggat taacgagtgg taaattttaa cttcaataga aaaaatattc tgttattttt
    32101 aaacaattaa ttagtcataa agtataaaat gtgtattata taagttaatc agtactttat
    32161 gaaggaagag gagagaaaga tacaatgaat aatttagatg atataccagt tatgccaact
    32221 ttgacacttg acccatttgg agaagaatca aacactaatg atatagataa ttctgactta
    32281 ttaatgaaaa aggatgaaaa agacccagaa gaagaaaaac tgtctgaatc tgaacgcaag
    32341 atggtaaagg aatttgcgga aaaaatagat attacaaata caaatatgat tttgcaatat
    32401 ggagctggtg ctcaaaaaaa ggtagcaagt ttttctgaaa cagctttaaa gagtgtaaaa
    32461 acaagagatt taggagaagt tggagatatg cttacaaatc tagttactga tttaaaagca
    32521 ttttcagcag atgaaaaaga acaaagtggt tttttaggaa tttttaaaaa ggcaaataat
    32581 aaaatttcaa atttaaaaac taagtatgat agtgcagagg taaatgtgga taaagtaagt
    32641 aaagagcttc aaaagcatca ggttaaaata ttgaaagata tagcaatgct tgataagatg
    32701 tatgaattga acttagctta ctcaaaagag cttactatgt atattatagc aggaaaacaa
    32761 aaattaaagg atatgaaaga atatgaaatg ccaaagttga gagaaaaagc tcgtttatct
    32821 ggttcaacag aagatgctca atctgtaaat gatatggttt cactttgtga cagatttgaa
    32881 aagaaaattc atgacttaga attaactaga atggtatcgc ttcaaatggc tcctcagata
    32941 agattggttc aaaatagtaa taatctgatg gcagaaaaga ttcaatcaac tatagtaaat
    33001 accattccat tatggaaaaa tcaaatagtg cttgctcttg gaatttctca cttaaatcaa
    33061 gctatgaaag cacagcgtga agttagtaat atgacaaatg agcttttatt gaagaatgca
    33121 gaaactttga agatgggaac aatagaaaca gcaaaagaat cagaaagagg aattgtagat
    33181 atagacacaa ttaagaaaac taatcaatct ctaatatcta ctattgatga ggtagttaaa
    33241 atacaacatg atggtagaat aaagagacaa gaagcagaag ttgaattatc aaaaatagaa
    33301 aatgaactta aaagtaagct tcttgaattt acagttaaat aaaaattata ctaatataaa
    33361 aagagactgt ctcaaaatag agataagtct ctttttcatt gtttaaatga ttaaagtata
    33421 tttatattat aaatttctat tatcttttaa cataacatca tgagctccac cttcaactat
    33481 actagtagaa gatacaagtg taagtttagc atttttttga agttcaggaa tgctaagtgc
    33541 tccacagtta cacatagtag aacgaacttt acttaaagaa agagttacat tgtctttcaa
    33601 agaaccagca taaggcacat aagagtcaac accttcttca aaagaaagtt tcttatctcc
    33661 accaaggtca tatctttgcc agtttctagc acgagcagaa ccttctcccc aatactcttt
    33721 catataacta ccatttatat taactttgtt agttggactc tcatcaaaac gagagaagta
    33781 tctaccaagc attataaaat ctgcacccat agctaaagct aaagttatat gatggtcata
    33841 aacgatacca ccatcagagc aaattggaat ataaatacct gttttttcaa agtattcgtc
    33901 tctagcttga gcaacttcaa ttatagaagt agcttgtcca cgtcctatac ctttttgttc
    33961 acgagttata catatagaac caccaccaat accaacttta acgaagtcag caccagcttc
    34021 agctaggtaa aggaatccct cgcggtcaac tacatttcca gctcctacct taactgtatc
    34081 accatatttt tcacgaatga aatcaagtgt tattttttgc cactcagaaa aaccttctga
    34141 agaatctata cataaaacat cagcaccagc ttcaactaaa gcaggaacac gttcagcaaa
    34201 gtctctagta ttaataccag ctccaactac atatctctta gaagaatcta aaagttcaag
    34261 tggattttct ttatgagaag agtaatcttt acggaatacc atatacataa gattaccatt
    34321 atcatcaagt acaggaagag aattaagttt gtggtcccat ataatgttgt ttgcttcctt
    34381 taaagtaaca tctttgttag cataaactat agatgacata ggagtcataa attcactaac
    34441 tttagtatct aaatccatac gacttatacg ataatcacga cttgcaacaa tacccaataa
    34501 tttaccagtt gaagttccat cttcagtaac agctactgta gaatgtccag ttttttcttt
    34561 taagtcaagt atgtctttta atgtgttatc aggtttaata tttgaatcac ttacaacaaa
    34621 accagctttg tgggatttaa cacgtgctac catagcagct tcattttcaa tagtttgaga
    34681 accgtatata aatgaaatac caccttcttt agcaagtgca atagccattt tgtcatcaga
    34741 aacagattgc ataatagcag aagtcaaagg tatattcata taaatatctg cttcctcgcc
    34801 ctttttaaat ttagtaacag gtgtttttaa acttacatta gcaggtatac aatcagtaga
    34861 agagaaacct ggtacaagaa ggtattcact gaaagtatgt gatggtgtat caaaataaaa
    34921 cgccattttt taatcactcc ttatattttt atacttttta taattgatag tattaattat
    34981 aacttattct ttaattttat taaagtattt tgtcaaatat tgagattttt gaaaaattta
    35041 aactatttta attaaatttg tttaatatat attgccttat agggtaaagt tgtatattaa
    35101 ataatgttta gtgtttaata gtagctaatt taaaattaat ctttagtgat tcatataatt
    35161 acatttgtat agtttatatt gataatatta ctagagagtg cataaacctc aaaaatacag
    35221 ataaattaaa tctatattag tatatccaaa agtacattta ataaatttat ttaactgaat
    35281 tattattttc atttctatta aatttaaata ttttataaaa atagtattat ttacaaaatc
    35341 atatactcca aaatactaca taaataaaat taaacaaaaa ctaaaatgac aaatgtcatt
    35401 atctaaagtg acatagaaac ggtttcttaa agttgttact ataaaatata aactctataa
    35461 taaaagtata acaaaaggga atcatatcga tatagaatat aattcaagag gaggtaaaat
    35521 tatgagtagt tctaatcaat cgacaataaa gacatctata cagcgagtag ggaaattttt
    35581 aagtggtatg gtaatgccaa atataggagc ttttatagca tggggtctta taacagcatt
    35641 atttatacca acaggatgga tgcctaatga aaaattgagt actataggcg acccaatgat
    35701 aaaatacctt ttaccactat tgatagctta tactggtggt aaagcaatag caggacaaag
    35761 aggtggcgta ataggtgcag cagcagctat gggagtaatt gttggtgcag atataccaat
    35821 gtttatcgga gcaatgataa tgggaccatt tgcaggatgg gtaattaaga agtttgataa
    35881 atttgtagat ggaaagatac caacaggatt tgaaatgtta gtaaataact tttcaatagg
    35941 tataataggt atgcttcttg caatactagg attttatgca attggaccag caatagtagc
    36001 agggacagct cttatagaat ctggagttca atttatagtt agcaaaagtt tattgcctct
    36061 agtttcagta tttatagaac ctggaaaagt attattctta aacaatgcaa taaatcatgg
    36121 tatattaggg cctataggta tagctgaggc aaaagaagca ggtaaatcta taatgttctt
    36181 actagaaagt aaccctggac caggtttagg agttctatta gcatattgga tgtttagtag
    36241 aggaagtgta aaacaatcag caccaggagc tgtaataata catttctttg gtggtatcca
    36301 tgaaatttac ttcccatata ttttaatgaa tccagtttta atattggcta caatggcagg
    36361 tggagcagca ggaatattga ctttttcaat attaggagct gggcttgtag cagcaccatc
    36421 accaggaagt atatttgcac ttatggcact agctccaaag ggaggattac ttccagtatt
    36481 agcaggagtt gcagtggcaa cagttgtatc attcttagtg gcagcaccat ttgttaagag
    36541 agcttcagca aatcaaagtg aagaagacag tacttcttta gaagaagcta aagcaaaaat
    36601 gagtgatatg aaatctgcaa gtaagaactc tgagaaaaat attgaggaaa aacagcttga
    36661 agtaaatgag attaagaaga ttgtatttgc atgtgatgca ggaatgggtt cgagtgcaat
    36721 gggagcttcc agatttaaaa atagaataaa aaacttagac ttaaatatag aaattacaaa
    36781 ttcatctgtt gataatcttc cagatgatac acaaatagta gtaactcaca atacattagt
    36841 tgaaagagtt gctaagaata attcaagtgt tgagatagtt tcaataaaca attttttaaa
    36901 tgacccaaat ttagatgcat tatttaaaag attagaatct aaataaaaat aaaaagtacc
    36961 actaattagg agtggataat ttgaaaaaaa agaagattac atctaggcaa aaaaagataa
    37021 tactaatgat agtagaaaat tcaaagaaaa acattccaat aactatttct gagattgcag
    37081 ggactttaga gctaagttca agaactgtac ttagagatat gtctggaatt gaaaaatggt
    37141 ttgatgaaaa tgattttaac tttgtaaaaa aaccaggagt gggattgatt ttagaggaaa
    37201 atatagaaaa tcaaaacttt ataattgagt tgttagaaga agaaaaaata gaaaaagaat
    37261 acagcaagga agaaagaaat ttaattatat tatcaaagct actagtttct aacgagccag
    37321 taaaatccta ttattttgca aaaatattaa aggtatctga aggggtactg aataatgatt
    37381 ttgccttagc aagtaaatgg ttggaaagat ttgatattga gcttgttaga aaaccagggc
    37441 ttggtgtcta cttaaaaggt caagaaaaaa actttagaga ggcatatgta aatcttatat
    37501 acgattcatt caatgaaaaa gaaatactag atatggtcag aaatataagt gaaaatatac
    37561 aaacagataa agcaatagaa attttaagtg aaaatagact tttaaattta atggacaggt
    37621 gcataataag aaaagttgaa gctacattaa caaagaagct ttcagattta gatgtaaatt
    37681 tagctgatag tgcatatata gggcttgtag ttcatatttc attagcctta cagaggataa
    37741 aaaatggtga aaatataact atggataaag agtttctaaa agaattatct ataacagaag
    37801 agtttaaatt agctaaagaa atcgtaaaag gtatggaaac agattttaac atggatatac
    37861 cagtagatga ggttggatat ataactatgc atataagagg tgcaaagcaa aggtcatctt
    37921 caaaccataa ggctttaaac ttagatgata tagaaataat ggaaattaca aataagatga
    37981 tagatttggc ggaagatgaa tttaaaataa gtttaaaaaa tgacgagagg ttatttaaag
    38041 acctagcaaa tcacttaggg ccttctataa atagattgaa tatgggatta gaaataagaa
    38101 atcctctttt agatgaaata aaaagcaagt attcatatgc ctatgatgga gttgaaaaga
    38161 tttctagaat tataaaagat aaactaaata taaattcaat acctgaatca gaaataggat
    38221 atattgcaat gcactttgca tcagccatag aaaaaaatct tatgatgaat actaatataa
    38281 atattgttgt ggcatgtccc actggaatag gtacttcaag attcttatca actaagattg
    38341 aaaataaatt tcctaattta aatatacttg aaaccatatc agctataaac atagatgaag
    38401 agtatttaaa agaaaaagat gttgatttaa tagtgtctac agttgagtta aatacaagtt
    38461 taaattatat atgtgtaggt ccatttatga gtttagatga tgaacagata atcaaggaaa
    38521 aaataaaatc tatagctcaa aataaattaa tcaatttaaa tgcgaaaaat gatactaaaa
    38581 gtaagaataa agtatatgaa cagataacag aaagtatgaa tataggaaaa gatattcttc
    38641 aatttttaga ggaaattaga tttgaaaaat ttgaaagtaa agatttaagt gagcttattg
    38701 aagactcatc aaaaatattt gcaaaatcta gtgaagatat aatctctata aaagaatcgt
    38761 taaaagaaag attaaaaatc tcaattccat atatagaaga atcaaaaatt ctattgttac
    38821 attgtatgag tgaaagaata gatattatga agttatcaat aataaaacta gagaatagaa
    38881 tcgtgctaga ttcaaatgaa gaaatcgata atgtagtttt tatgttactt cctaaaaatt
    38941 caccaagtta tcaaagacaa ataatgagtg aaataagtgg ttcattaata gacaatataa
    39001 tttttactaa taagataaat aaattctcta tagaagagat gacattagag gtaaaagata
    39061 tagtttttaa tttttataca aacagattaa aagcatttat agataaataa aattaatatt
    39121 aaaaaggaag gttgatacta tgagtaaagt attgaatgag aataatatat ttttagggtt
    39181 agatagtgtt tcaaaggaag aagctataac tttagcagga agaaaattag ttgaaaatgg
    39241 atatgtaaaa gaagaatata taccagcaat gttagaaaga gaaaaagtaa tgactacata
    39301 tatgggaatg ggagttgcta ttccacatgg tgtaaatgaa gcaaaaaaag aaatattatc
    39361 ttctggaata gttatattac aattccctaa tggtatagat tttgatggag aaaaagcata
    39421 tttattaata ggaatagctg gtgttggaga tgaacattta gaaatactat ctaatatagc
    39481 agtagtttta gatgatgact taacagaaag actaaaaaat tcaaatgaca aacaagcttt
    39541 tatggaagct tttgccaact aatcttagga gggatttaaa atgaaaaagg cgattcagtt
    39601 tggagcagga aatattggaa gaggttttat tggaagcttg ttagttaagt cgggttatca
    39661 tgtagtattt gctgatgtaa atgaggagat attaaactca ataaacaaag ataaaaagta
    39721 tactatacac ataagagatg tagaatgtat agatgaagta attgataata taagtgcggt
    39781 ttcatctata aaagaagaaa taatagatga gattgtacag gctgaaataa taactacagc
    39841 agttggacct ttagttttga ctaaaatagc ctctacaatt gcaaaaggta taaaagctag
    39901 aaaagaaaag ggattaacaa gtaatctaaa tataatagca tgtgaaaatg caatatacgc
    39961 aagttcttct ttaaaagaag aggtacttaa gtatttaaat aaggaagaag tagaatattt
    40021 agagatgtat gttggttttc caaactgttc agttgataga atagttcctc ctggtaaaaa
    40081 cgaaaatcct cttgatgtaa cagttgaaaa tttttatgag tggaatgttg aaaaacaagg
    40141 atttaaaggg gaaataccaa ctatagttgg aatgaattta gctgataacc ttatggctta
    40201 tatagaacgt aaattgttta cactgaatac aggtcatgct ataacagctt atataggata
    40261 tttaaaagga tataagacta tagaagagag tataaaagat aaatttatct gtgatatagt
    40321 caaaagtgca atggtagaaa gtggagaagg attgattaaa aaatacaatt ttgactcaga
    40381 agtgcactat aaatatattg ataaaatttt aaatagattt aaaaatccat atcttaatga
    40441 tgatgtatta agagtcggaa gggaaccatt aagaaaacta agtgataaag atagattgat
    40501 taaaccttta atgacagcaa aatcatatgg attatcagtt gacaatttga ttcttggaat
    40561 aggtgcagcg cttcattata ataatagtga agatactcaa agtgtagaat tacaagagtt
    40621 gataaagtct attggggtaa aaaaagccgt tgctaaaata gctaatataa gcaatgacga
    40681 agaaatttta aataatatcg aaaaaagtta tatatttatg aaaaatttat aaatttactt
    40741 gaaaaaaagt agttctgata aagtaactat aatatattat tctacacagt ttgaattatt
    40801 tgttgaataa tagagaatta taatatataa ttaaaaggaa taaattaata tatttacctg
    40861 tatatgtttg ataatatggt tcaaatgttt ctactaagct accattaata gcttgactac
    40921 aggagtgaag aagatatagt ttatacttat tttaaattat atattaagtt aattactttg
    40981 aattttaagc tcctgtctat gatatagaca ggagcttttt tagtatttta atataaaata
    41041 cttacaagaa tagatagcaa aattagaaca aatataattt ttttattaat ataattttag
    41101 aaagggctag gtggaagttc aatgaaggct ttacaagaaa agatattgag agaaggcagt
    41161 gtttcaggaa atgatatact aaaagttgat agttttttaa accatcaaat tgatgtagct
    41221 tttttaaatg aaataggaag agaatttaaa gagagattta aaggagaaaa ggtggataag
    41281 atatttacaa tagaggcttc aggaatagct atagcatcaa ttgtttcaca gtattttgat
    41341 aatgcacctg tagtatttgc aaaaaaatca gaatctaaaa atttggataa agatgtatat
    41401 gaaactaatg tatattcatt tactaaggca agagagtatt cagttaaagt ttctaaaaag
    41461 tacataaaca aaggcgaaaa tatattgata gtggatgatt ttttggcaaa tggaagagct
    41521 gctcttggac ttaaagattt aattgagcaa gcagaagcta acttagttgg agtaggaatt
    41581 gtcattgaaa aaggatttca agctggagga gcacttttaa aggctaatga tgtaagatta
    41641 gagtctttgg cagtagttga atctatagat aatggaactg taaaatttag gtaatttatg
    41701 atattatata attgtgttta gcacatattt taactatgta attatggagg attttttatg
    41761 aggtttttta tagacacagc caatattgaa gaaataaaag aagcaaatga tttaggtgta
    41821 atatgtggtg taactactaa tccgtctcta atagctaaag aaggtagaga ttttatagag
    41881 gtagttaaag aaataagtga aatagtagat gggccaataa gcgcagaagt aataagttta
    41941 gaacataaag gtatgataga ggaagcagaa aaattatcta agatacataa aaatatagtt
    42001 ataaaaatac ctatgacagc agaaggtcta aaagcggtga aagtactatc aagtaaaggt
    42061 ataaagacta atgtaacgct tatattttct gctggacaag ctcttttagc tgctagagct
    42121 ggagctacat atgtaagtcc ttttgttggt agattggatg atatatcaca aaatggtctt
    42181 gatttaattg aagaaatcgt tgatatattt agtgtaaata gtattgaggc tcaaataata
    42241 gttgctagtg ttagaaatcc aatacatgtt ttacaagctg ctagaatggg agcagatata
    42301 gcaacagttc ctcttaaagt tataaaccaa atgattaagc atcctttgac agacaaagga
    42361 atagatagct ttatgaagga ttgggaaggt gcttttttaa aattataatt ataaactacg
    42421 ctttataagt atattaaaga ggttattatg gaaattctaa gtaataaggc taggctattt
    42481 tgcctagtct tattttggtg tataatatat tgaaacatat tattgaaata gattaatttt
    42541 ttaagtgagg aggcgaaaaa tggataaaat attaagagag atacaaagag aagataaaaa
    42601 aaatccatat actgaccaag aacttgctga aatgttaaat gtagctaggt ctgaagtgat
    42661 atcagttagg aaaaaaaata atattttaga ttcgagagaa agaagaaaaa aaatattaat
    42721 aaaagatata tcaaaaattg taagtgaaaa tcctcaaatg tctgaaagaa aaataactga
    42781 aatattaata gaaaatggat ataatatatc gagaagtgca gtttcaaaat tattaaaaga
    42841 ggaaaattta aatcagtcaa agattgggat taaggataaa gacccagcat catcaggaaa
    42901 gtatgtgaag gtgagtaaag ttgtagaaga agatggattt gaagaattga taggtataaa
    42961 gggaagtttg aaagaaaagg taaatctagc taagtcagca ataatgtatc ctcctaatgg
    43021 ccttcatact ataatatatg gagaaacagg agtaggtaaa agtgaattag ctacatgtat
    43081 gtataaatat gctataaaaa acaacattaa agaagagaac agcccattta tagtgtttaa
    43141 ctgtgcagac tatgcagaaa atcctaatct attgatagct caactctttg gggtagtaaa
    43201 aggtgcatat acaggagcag atgcaaatag agaaggtctt gtagaacaag ctaataatgg
    43261 aatattgttt ttagatgaaa ttcatagatt gcctgctgct gggcaagaaa tcttattttc
    43321 attaatagat agaggagaat ttagaaggtt aggagaaagt agttctaata gaaaggcaaa
    43381 tgttctcata ataagtgcta caacagaaaa tccagattct aatttacttc aaacatttag
    43441 aagacgtata ccaatggtaa taaatttacc taatatgagt gaaagaccaa aatctgaaag
    43501 atatgaaata ataataaagt tttttgaaag agaagcaaag cgagtaaaca agaattttat
    43561 aataactaaa gaagttatgt gtgcacttat gaattataag tgtactggaa atataggtca
    43621 gttaaagagt gatatacaag taacttgtgc tagagctttt agtaaatcga tattttctag
    43681 cgataaggta attatagatt tagatagttt aagggattat ataaaaagtg gatattatga
    43741 ttgtaattgt aaaaatgaaa agtattctga gttttcagtt gaggatatat atatagatat
    43801 tagtgaattg gtgagtggaa aaaatagaat tttatctgat aatgagataa gcgagatata
    43861 taattatgca gaaaaagagc ttaaggtttt ggaaagtaag tgcttttcag aagaagagtt
    43921 aaaaaatgca tttatagaaa agttagataa gaagtttaat gctattaaaa acaataaaaa
    43981 tttatctgat agaagaagtc gaatagactg gggaggacaa ttaaaagagt ctacactgga
    44041 gataatggat aaggtaattg tatttattaa ggagcagttt aagcatgtaa atcaaggtct
    44101 gtacttggct ctagcaattc atattgagca tgctataggt aggataaaag atggaaaaac
    44161 tataataaat ccgagtttag ataaaataaa agcatcaatg ccaatggaat atgagttgtc
    44221 aagatatata atgggtatag tagaagattt gacacaagta acatttccag aagatgagct
    44281 tggatatttg gcttattata taaataaatt ttgttataat gaggaaagta taaaagataa
    44341 agttaaagta gtaatagtta cgcatggtaa agttggaatt gaaatgtcta aagttgtaaa
    44401 tcatatttta ggtatagaat gtacactagg tatagaaata gcacttacag attcaccatc
    44461 agaggggata gagcatgtat tagaagagct tcaaaagata gaagctagaa aaggtattct
    44521 agttctaatt gatatgggtt ctttggtaat tcttggtgat gaggttgaaa aaaggcttgg
    44581 aattagatgt aaaactgtaa atagagtaga tacattattg gcaatggaag caggaaaact
    44641 tgcaaccatt gaaggaaaaa gtttagatgg tataatagct gatttaaaga aaaataagaa
    44701 ttatgctatg ataaatacaa ataaatttag ttacagaaaa aacgagtatg gaaagaaaaa
    44761 tgtaatcatt actttatgtt tgagtggtgt aggtacagct ttaaatttaa aagagcatat
    44821 agaaaaacaa attaaggaat atggcacttt aattgaagta aagccagttg cttttttaaa
    44881 taataattta gaagaagagt taaatgatat tgaagctgaa tataatatta ttgctatctg
    44941 tggaactatt gatatagatt ataaaaacgt gccttttatt tcttataatg aggtacttgg
    45001 taaaaatggt attaataaag tattagagca tctaaatagt aaacaaccag ctttaaatat
    45061 tgaaaaccag ttgaataatt taatacatga agatttgatt ttatacaatt ttgaaggaat
    45121 ttcaaaggag tatataattg atactcttgt ttcaaaactt gaagaaggtg gatatgttga
    45181 ttcaaagtat atattaagtg tatataaaag agaagcaatg ggaagtgttg taatggcctc
    45241 taaggtagcc gtgccacatg gacttccaga aaacgttata aagcctgcaa tagcaatcgc
    45301 aaggttgaat aaacctatag tatgggacaa taaatttatg gtggatttgg tggtactact
    45361 ggcacttaaa gaaaataata agaaagaaat acgaagctta ttttcaaaga ttaatgacca
    45421 gcatacttta gaagttttat taaatactga agataaaaat gaaataaaag aacttttaag
    45481 ctaattatta gtaccgagaa gaaagacaaa aaaatttgct ttagtataaa atttaaaatt
    45541 actcaattgc taagta
//
LOCUS       DACTGW010000005        43987 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.005, whole genome shotgun sequence.
ACCESSION   DACTGW010000005 DACTGW010000000
VERSION     DACTGW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.005"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(540..980)
                     /locus_tag="I9573_000218"
     CDS             complement(540..980)
                     /locus_tag="I9573_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAT4794864.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(1052..1879)
                     /locus_tag="I9573_000219"
     CDS             complement(1052..1879)
                     /locus_tag="I9573_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794865.1"
                     /translation="MEFESGIKLGKLTLIKYLGRNKHSKKVWLCQCECGNKVERVENN
                     LKQSIKNEKPPHCGCSPNWKGQNKRFKDITGKTFGKLTVLKMSGKDKYSHNLWLCQCE
                     CGNRTITSTSALEQGKAQSCGCIRKEILLERSTTHNKSNDSLYRVYHRMIKRCTNKSN
                     KDYNYYGGRGIEVCNEWLEDFINFYNWSIENGYRKGLTIDRINVNGNYEPSNCRWVTW
                     EVQRNNKRNSIRVNYKGEMITLKQCAENLNVKYLFLYNQLVTKKIPLEEVIKNIGME"
     gene            complement(1938..2408)
                     /locus_tag="I9573_000220"
     CDS             complement(1938..2408)
                     /locus_tag="I9573_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HAT4794866.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVESFALLNGML"
     gene            complement(2425..3735)
                     /locus_tag="I9573_000221"
     CDS             complement(2425..3735)
                     /locus_tag="I9573_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAT4794867.1"
                     /translation="MALGGGTFVTQNKILPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEAFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVILLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSIENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAIKVINAMSKLYMTVSVN"
     gene            complement(3905..4342)
                     /locus_tag="I9573_000222"
     CDS             complement(3905..4342)
                     /locus_tag="I9573_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794868.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            complement(4691..5497)
                     /locus_tag="I9573_000223"
     CDS             complement(4691..5497)
                     /locus_tag="I9573_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794869.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNKLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            complement(5607..5897)
                     /locus_tag="I9573_000224"
     CDS             complement(5607..5897)
                     /locus_tag="I9573_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAT4794870.1"
                     /translation="MARWGSVDFREFKRVCKKMEKFTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLREGWSGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            complement(5897..6244)
                     /locus_tag="I9573_000225"
     CDS             complement(5897..6244)
                     /locus_tag="I9573_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794871.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(6238..6639)
                     /locus_tag="I9573_000226"
     CDS             complement(6238..6639)
                     /locus_tag="I9573_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794872.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            complement(6649..6882)
                     /locus_tag="I9573_000227"
     CDS             complement(6649..6882)
                     /locus_tag="I9573_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794873.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(6901..7791)
                     /locus_tag="I9573_000228"
     CDS             complement(6901..7791)
                     /locus_tag="I9573_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HAT4794874.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATENNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            complement(7803..8366)
                     /locus_tag="I9573_000229"
     CDS             complement(7803..8366)
                     /locus_tag="I9573_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HAT4794875.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTVTQEDYVNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDMEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(8434..8682)
                     /locus_tag="I9573_000230"
     CDS             complement(8434..8682)
                     /locus_tag="I9573_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794876.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            complement(8684..10219)
                     /locus_tag="I9573_000231"
     CDS             complement(8684..10219)
                     /locus_tag="I9573_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAT4794877.1"
                     /translation="MKHKDYWRKRFEQLEEVQNNKSVKYYLELEKKYKLAMNSIEKDI
                     LSWYNRFAENEGISLLEAKKLLNTRELEEFKWNVEEYIKYGKENAINQKWMKELENAS
                     ARLHITRLEALKLQIQQHVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRPTLINELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     NGE"
     gene            complement(10209..11651)
                     /locus_tag="I9573_000232"
     CDS             complement(10209..11651)
                     /locus_tag="I9573_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAT4794878.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEMQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKSVQITFNHSMIINESEKIDMASKSIGIISDETIVSNHPWVEDVNDE
                     LERLKKQEETQKEYDDLIPTNNQEGVIDET"
     gene            complement(11657..12910)
                     /locus_tag="I9573_000233"
     CDS             complement(11657..12910)
                     /locus_tag="I9573_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAT4794879.1"
                     /translation="MVIEMIVRINFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANAIL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSRGLEKTLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(12888..13580)
                     /locus_tag="I9573_000234"
     CDS             complement(12888..13580)
                     /locus_tag="I9573_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4794880.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWSSE
                     KKKGAPINKRGAPFSNKNSVGYGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHEITENSEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVANLNKDDIGDDEL
                     KISVDYGDRNDS"
     gene            complement(13650..14159)
                     /locus_tag="I9573_000235"
     CDS             complement(13650..14159)
                     /locus_tag="I9573_000235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794881.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            complement(14180..15154)
                     /locus_tag="I9573_000236"
     CDS             complement(14180..15154)
                     /locus_tag="I9573_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794882.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFEKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            complement(15349..15898)
                     /locus_tag="I9573_000237"
                     /pseudo
     CDS             complement(15349..15898)
                     /locus_tag="I9573_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            complement(16181..16660)
                     /locus_tag="I9573_000238"
     CDS             complement(16181..16660)
                     /locus_tag="I9573_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794883.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMVKELGVYLFGIDGLKLTV"
     gene            complement(16814..18184)
                     /locus_tag="I9573_000239"
     CDS             complement(16814..18184)
                     /locus_tag="I9573_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAT4794884.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCTLTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKNGAENA
                     IHNKIKDICISLKAEDYLNMPRKIDNKIEIHLDSKILKYYKELEKEKILELDRDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEAIENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(18133..18459)
                     /locus_tag="I9573_000240"
     CDS             complement(18133..18459)
                     /locus_tag="I9573_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HAT4794885.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(18741..21158)
                     /locus_tag="I9573_000241"
     CDS             complement(18741..21158)
                     /locus_tag="I9573_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794886.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIENFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDTGVKKVNKSIFNGQLDNERNQIWAEAVELCKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITENWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            complement(21170..23128)
                     /locus_tag="I9573_000242"
     CDS             complement(21170..23128)
                     /locus_tag="I9573_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HAT4794887.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTAISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWFAGEKWRLDVFNSHGKIYEASASHMFKVPIESIKKGDPLRQKGKISELAL
                     GYGGSIGALTSMGAIKMGLDEEELQPLVTTWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VKLQHGIKFIYNPGVLFIELPSGRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(23128..23700)
                     /locus_tag="I9573_000243"
     CDS             complement(23128..23700)
                     /locus_tag="I9573_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HAT4794888.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(23723..24295)
                     /locus_tag="I9573_000244"
     CDS             complement(23723..24295)
                     /locus_tag="I9573_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HAT4794889.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(24348..24683)
                     /locus_tag="I9573_000245"
     CDS             complement(24348..24683)
                     /locus_tag="I9573_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794890.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     RLNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            complement(24698..25864)
                     /locus_tag="I9573_000246"
     CDS             complement(24698..25864)
                     /locus_tag="I9573_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HAT4794891.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPLTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(25876..26313)
                     /locus_tag="I9573_000247"
     CDS             complement(25876..26313)
                     /locus_tag="I9573_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794892.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(26384..26533)
                     /locus_tag="I9573_000248"
     CDS             complement(26384..26533)
                     /locus_tag="I9573_000248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794893.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            complement(26514..26906)
                     /locus_tag="I9573_000249"
     CDS             complement(26514..26906)
                     /locus_tag="I9573_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4794894.1"
                     /translation="MDISESIAKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVEWGDEIAES"
     gene            complement(26937..27134)
                     /locus_tag="I9573_000250"
     CDS             complement(26937..27134)
                     /locus_tag="I9573_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4794895.1"
                     /translation="MLIGDNIKQMLRKRDIKPYKLAKELNIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            27210..27584
                     /locus_tag="I9573_000251"
     CDS             27210..27584
                     /locus_tag="I9573_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794896.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            complement(27594..27803)
                     /locus_tag="I9573_000252"
     CDS             complement(27594..27803)
                     /locus_tag="I9573_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794897.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(27850..28659)
                     /locus_tag="I9573_000253"
     CDS             complement(27850..28659)
                     /locus_tag="I9573_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HAT4794898.1"
                     /translation="MNNLQIFKNKTFGEIRVIELNGEFWFVGKDIAEQLGYKDTSDAL
                     KRHVDDEDKGVGEIPTPGGNQNMKVINESGLYSLILSSKLPSAKLFKRWVTNEILPSI
                     RSTGTYNMIDLQTKLPTTYKEALQHLIEQVEVNEQLQLESKMKDQVIKELKPKADYTD
                     MILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHSET
                     IDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKGYSYQTSILG"
     gene            complement(28707..28910)
                     /locus_tag="I9573_000254"
     CDS             complement(28707..28910)
                     /locus_tag="I9573_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4794899.1"
                     /translation="MKKRSLKAGRIEAGYTQEELAYKIGIAKSTYNLKENGKRNFTEK
                     EMIMISYILNKTMDELFLEKRLT"
     gene            29087..29428
                     /locus_tag="I9573_000255"
     CDS             29087..29428
                     /locus_tag="I9573_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4794900.1"
                     /translation="MATFGERFKQLREEKKLTQDELVSKFNKVYFTSFNKSTISQYEN
                     NKRKPEINILENWADFFDVSIDYLLGRTLVRNHIDTVAAHRVNPHKDLPEEAQEQLND
                     YIEFLMNKYKK"
     gene            29440..29988
                     /locus_tag="I9573_000256"
     CDS             29440..29988
                     /locus_tag="I9573_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4794901.1"
                     /translation="MLILKKLRTEKGISLDKLSTDLNINKSTLSRIENGLREPKESFI
                     KDCSDYFGVSTDYLIGKINIDDSNKLTKCLNSTFPIRLKELRKKKELTQAELSKLLNC
                     SLSKIAMLETSKREPVKEDLLRISEFFNVSVDYLLGKTSIENYITTDEISKIIKSYES
                     LPKEAQEHINSYIEFLVDRYKK"
     gene            30064..30570
                     /locus_tag="I9573_000257"
     CDS             30064..30570
                     /locus_tag="I9573_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAT4794902.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            31190..32059
                     /locus_tag="I9573_000258"
     CDS             31190..32059
                     /locus_tag="I9573_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HAT4794903.1"
                     /translation="MIKNFDEWKEYDGFSYGSGRSEKIWLINEKTKDIGLFKFPKTKE
                     TGEYWAEKLACEIASLLKIPCANVEIGTYNKRIGSMSYLINKNDEKLIEGVWLINKYY
                     PCYNTDKLYDYKNNEYYSLKMIFKSIEEYNIKNDFLKMLIFDYLIGNRDRHQNNWALL
                     ESQNKITLSPLYDNGSSLCCYVNIENIDSFFTDHQRFEALINTRSRSRIRIDGKNKKE
                     PTHLEVLKYLNSNYYEQTIDFVEKIINLMNEVNINTLIDNYSENIISPKLKKLLKIYL
                     KEKVLRIKNIYNL"
     gene            32072..32779
                     /locus_tag="I9573_000259"
     CDS             32072..32779
                     /locus_tag="I9573_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAT4794904.1"
                     /translation="MSIKNGKDFLYVIWKDPKSRRQFIIGKLAKNGLYEFSYEYEIDE
                     AIKAGFNALISFDDISKTYYSEDLFSPFASRLPDKKRKDINKILDKYDMSEYDTYELL
                     KRSGARLPIDTLEFVDPIPLDIDKNIIRYFYIAGVRHYAGCEGHECDNSIELESNEAL
                     VLELEPENKFDPHAIKVFDKKENHIGYIPRYYSGELTSIINRGLKYNCIIKFVNKENN
                     CDECIKVKLEILFDEYN"
     gene            32858..33988
                     /locus_tag="I9573_000260"
     CDS             32858..33988
                     /locus_tag="I9573_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAT4794905.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(34346..36034)
                     /gene="ilvB"
                     /locus_tag="I9573_000261"
     CDS             complement(34346..36034)
                     /gene="ilvB"
                     /locus_tag="I9573_000261"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HAT4794906.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWSAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKAGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSNMEELGKVLDVIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(36087..37082)
                     /gene="ilvC"
                     /locus_tag="I9573_000262"
     CDS             complement(36087..37082)
                     /gene="ilvC"
                     /locus_tag="I9573_000262"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAT4794907.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            complement(37352..37591)
                     /locus_tag="I9573_000263"
     CDS             complement(37352..37591)
                     /locus_tag="I9573_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794908.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            complement(38122..38883)
                     /locus_tag="I9573_000264"
     CDS             complement(38122..38883)
                     /locus_tag="I9573_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAT4794909.1"
                     /translation="MNTGSKDIDTLNVNFRGIEIKAVLVYKNRKNISIKIDPFGKIIV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLKDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERTVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     gene            complement(38888..39709)
                     /locus_tag="I9573_000265"
     CDS             complement(38888..39709)
                     /locus_tag="I9573_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAT4794910.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANYER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKRETGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            complement(39751..40506)
                     /locus_tag="I9573_000266"
     CDS             complement(39751..40506)
                     /locus_tag="I9573_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAT4794911.1"
                     /translation="MTTYKKLKKSIFKISIFSICLLVSLVDINPIFAGSSSNETSNSD
                     TIKVLNAKSLNFKQGNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEDSSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDFPVKDFESINKYQDFYFNKN
                     GNLVISFEEYEVVPGYMGAVEFVIPNKVIKRLR"
     gene            complement(40549..41418)
                     /locus_tag="I9573_000267"
     CDS             complement(40549..41418)
                     /locus_tag="I9573_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-V factor rsiV"
                     /protein_id="HAT4794912.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGVIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKAHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            complement(41430..41912)
                     /locus_tag="I9573_000268"
     CDS             complement(41430..41912)
                     /locus_tag="I9573_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAT4794913.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            complement(41964..42920)
                     /locus_tag="I9573_000269"
     CDS             complement(41964..42920)
                     /locus_tag="I9573_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAT4794914.1"
                     /translation="MNKKLYIGMVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVL
                     SATNNYINGQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVD
                     SKYQELEDKMNLNTSYKEKMDKAGVDKEYLKQEISRDLAIDKNKKAFEDRINISDNDM
                     EAYYTSHKKDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESF
                     ESLAKKYSDDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVK
                     VTDKRERQKSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            complement(42989..43822)
                     /locus_tag="I9573_000270"
     CDS             complement(42989..43822)
                     /locus_tag="I9573_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HAT4794915.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
BASE COUNT        13646 a         7942 c         4553 g        17846 t
ORIGIN      
        1 acagtagagt gaagttcata atttaaacaa ttagtaatta tttaaatttg tggaacttga
       61 ttttaaaatc aaattcccag ccacttttac tcttgccacg agttgagtgg ctttttactt
      121 ttagaaatac tttacaaatt aagcaaaata ttaaactagc aataacgcca gctattacat
      181 taagtaaaaa gttattcata cttcccacct cctttcatta ggaagtaggt tttatcccag
      241 tatgaactcc actctataaa ttgtagatta catcttcttg ctaaaaatat tataacatat
      301 aattattaca tattttacct attctatatt tattttttta ttttgctatc ttcttcgtcc
      361 cctctttctc tctctttcag cttctttcat tgcttcctct tcatcttcta tctttataag
      421 tattgaggcg gctgctaacg ctctctcatt aacttctaaa ttcatatatt cacttggctt
      481 ccactttaat ttttgaatac aataatgagt gatgctagca tcaaaatcgc cacctctgat
      541 tagttttttg cttcttctac tttatcctca aaagatgtat caaatccatt gacttcattc
      601 acttttactg tataattgac atactcacct gctgtaagca ttgtctttaa taactgagct
      661 tctcccatta ctccataact attttggagt tcggcatcct ttaaatctgg aaatactgta
      721 gatgctacac ataattcagc tacataacta ttgtagtcaa tttcacttgt atattgtcca
      781 gtatgcttac cattgttacc aatcactttt actcttttag tacactttct tcttagtgct
      841 tcgtcttctt cagatgataa aacttttaat tcccattcaa ctggtttccc ttctttatct
      901 aaaaatctgt tactcgctac atattttaca ttatcaacct ttattgcatt ttgacttaaa
      961 aaagcactta aattactcat attattctaa tctcctttta ttttaatttt tcatataaaa
     1021 aatacacatt tataacttat aaatgtgtat tttactccat acctatattt tttattactt
     1081 cttccaatgg aattttttta gtaactaatt gattataaag aaatagatac ttaacattca
     1141 aattttctgc acattgtttt aaagtaatca tttcaccttt ataattaact ctaatactat
     1201 ttcttttatt atttcgttga acttcccaag taacccatct acaatttgat ggttcgtaat
     1261 ttccattcac attaattcta tctattgtta aaccttttct atatccattt tctatagacc
     1321 agttataaaa atttataaaa tcctctagcc attcattgca tacttctatg cctctacctc
     1381 cataataatt ataatcttta ttactcttat ttgtgcacct tttaatcata cggtgataaa
     1441 ctctatataa gctatcatta gatttattat gcgttgtaga gcgctctagt aatatttcct
     1501 ttcttataca gccacaactt tgtgcttttc cttgttctaa tgcacttgta ctagttattg
     1561 ttctatttcc acattcacat tgacataacc ataaattgtg actatattta tctttcccag
     1621 acattttcaa tactgtaagc ttcccaaaag ttttcccagt tatatcttta aatctcttat
     1681 tttgtccttt ccagtttgga gaacaaccac aatgtggagg cttctcattt ttaattgatt
     1741 gctttaaatt attttctact ctctcaactt tattcccaca ctcacattga catagccaga
     1801 cttttttact atgtttattt ctccctaaat atttaattaa agtaagtttg cctaatttaa
     1861 ttccactttc aaattccatg aaatacctcc aaataattta tacttacatc ataatatttt
     1921 tcaagtataa ttcaatttta taacattcca ttaagtaatg caaaggattc aactaattcc
     1981 caatcctcaa aagtgaaatc catatcttca tctaaatact caccatcagc atcaaattta
     2041 gtaattattc cactgtccat attacaatct ttaagtacta ctgtctgtct tcctacagca
     2101 gatgtagggt cttcatttgt aacttgtatg tcaaaataaa tatcctcacc agtttcttta
     2161 tatctgtaaa gtaattctct aaaaatagaa gtattataat gaaatgttgc acttccagta
     2221 tttgtactcc cagttgtttt atttcccttt gttgttcttc ctagaattgg aacttcactt
     2281 ttattttttt ccattttagc ctctaaatct atagcttgca taaaattata tcttttacct
     2341 tctatagtta taaaacattc agctttcttt gcactaactg tatctttagc atttattgtt
     2401 tgagccatat tatcactctc ctctctaatt aactgaaaca gtcatataaa gcttactcat
     2461 agcatttatt accttaatag catcagatac tatgacagtt ttcttatcat ttccaagctc
     2521 tacactaaca tcatcagttt taaaatcttc tattgccctt atattctcta attctttatg
     2581 gtgtttaaca acatcattcc agaaacttat tcttcctgcc ttatcattcg gaactttacc
     2641 taaatacttt tcattaaata aagttgcaat atcattagca atttggtcaa gtactctaac
     2701 actttggtta cttgaaaaat cgtcattttt atcatctgta aatgatacaa aagtatttat
     2761 atcctctaac acatgaactt catctcccac cttgtggaat ataaatttac cagtttttaa
     2821 agcttcttca agttgtattt gtgtataatt aacatcaaca tcaaactcac catcatactt
     2881 tttattagta ttagatttat ttatatcgca tcctgctata gctccagtag cccaataaat
     2941 caagctagat tcaactaaat ctttatcttt aatcttattt tctatagaca ctacaccttc
     3001 ataatctgca tcacttttct tatatagtac tgtttgaaac ttagctccta ccttatctct
     3061 cattctcttt gtaaattcta caaataaact tttaatttct gttgttgtag ccaaacaccc
     3121 tagtgcatta aatgaataac tttctatttt atccaagaaa gcttggtact ctgctcctgt
     3181 cacagcttcg ccattggttc caccagtaaa tacaagtcct gcacttgctt ctagtgttgc
     3241 atccttcttc caagtgatat agtcattgtc ttgtaagtct gtaataacct ttgctatttg
     3301 agtatctacc ttcttattat ctaaaagtat tacaacatca aacttagcat tatcatctat
     3361 atttgttgta actgttactt ttaagtcatt tcctcttata ccactatatt ttgctgtggc
     3421 tatagtacaa ctggctttaa cgcctttatt taatttataa aaatatccca accttatatt
     3481 tttgaataaa tctctcaaac ctttcagctt ctcatgagta taatcatatc caaaatactt
     3541 cactgaatac ttctcaaaat catcactggt tacttggaat gcttcttcat ctatgcccca
     3601 atctaactct aaaggcattg caacaatacc tctatctgat aatgaactgg ttgccctctt
     3661 agctgagata aaatttatat atgcaccagg aagtatttta ttttgcgtta cgaatgttcc
     3721 tccacctaaa gccatctaac tcactccttt cataaattta tttattctat cctctacctc
     3781 tgagaaggaa tataactcat tttcttttaa aattgcattt aataaatctt ttctatttac
     3841 atacttctta gagttaacta tctgctcctt agtaaacttg tagttatctt ctttgcttaa
     3901 tgctttactc aaaattatca cctctcttca aaccaccgaa taactctact gtatccatct
     3961 tattggtatc attatttttt atagtaaaat agttataatc aacaaagaag tgaagaacat
     4021 tgtctataat ttcaaaattc atatttgtac ctcttactaa atctccatca atttctatat
     4081 actctaattc ctccagtagc atctcagcta tctcatttat ttcaaaattc ttatcttctg
     4141 aacgagggaa ataatgtaca tcaaaagaat ttttctttaa ttctctcccg cttggatatg
     4201 gtgtcttgct tggatttaaa ggaacaataa aaaaacaagg ttcattaata ccttgctcca
     4261 catcttcgct ataaattgta tatttttctc caaacgattt atctaacttt actgatattc
     4321 catctataat attattaagc atcaaacact cctttaagca atattaatag ttttttctct
     4381 attatcttat caacttgact ttgtagttcc atttctgaaa tagttaagaa atgttgtcct
     4441 ttaacccaac ccttgccatt tttagttcta tgaccaaatt caacatatgc attttgttat
     4501 cctaaaggct ttttatcctc tagctcttat agtttcctat aagttcggcg tacatcatca
     4561 acaaaataaa ctttatttag ttgcccagca ctcttggaga gattatattt attcactctc
     4621 tacgctctac ggaaacctat agcctatccg caatctatag gttttcctcg gtattggcat
     4681 atataattaa ttaaatattt ttccatgttc ttctatggac tatatttgat atagatgaat
     4741 atgttacagg atatatttca cttagttgtt ttattgtata tccatcagaa tacttttttc
     4801 taatttcttt tacatcctca atagataact tagctctgct ttcttcaata atcttaactt
     4861 tttctataag cttatttcct actaatccct tagaatatct acttcttaag caagagtatg
     4921 aaatacctgt tttttcagaa agctctatca atgtaatttc tttttcttca tgttttacta
     4981 atatatttgt tgacctattt ctacattgtg tctttatatc cacccatcta caattgcttg
     5041 attcataatt tccttcatta tttattctat caattgtaag tttctctgaa tatccattac
     5101 tcaatgccca atttgcaaaa ttatcatagc aaaaccattc atcacacaca attatattcc
     5161 tttcaccata tctattgtat cttttattat ttttatctgt acatctagat attataccct
     5221 tccatatact gtaaagtctt ttattttgaa ccttatattt ctttttaaat tggtatttgt
     5281 ctgttaaatt taatttatct tgttcttttt ttaaacatcc acaagattgc actaagccac
     5341 tagttaaaga gtcagttctt atttctttga aatttccaca atcacactta cataaccaat
     5401 atttcctatt tcttttaccg ctttttatct ctttagaaaa tttaatgact cttagccttc
     5461 caaatttttt tcctgtaata tctaaaaatt ttgccataaa atcacctctt ttatacatta
     5521 taaatcaaga gttattctat gtccatattc tacctacttt ataatgtaac actattacac
     5581 ttagcgttta ccgattttgc tgggttttat atgccccatt gtgttaaggc atattcagtt
     5641 ggattaacaa cttctataat ataattatta ccttgtttgt aaacaggaag cgacctagca
     5701 taagctactc cactccaacc ctctctcaaa aatcctgtat caactggtgt ccttctaatt
     5761 acttttccta agagtcttgc tgctaattct cttgctgcat ccttgcaaaa cttatctaaa
     5821 tcaatctttg taaatttctc catcttttta caaactcttt taaactctct aaaatcaaca
     5881 ctgccccatc tagccattat gctttatcct taaataactc aagtattatt tcttgatgat
     5941 ttggatatat agctgattct ccacttctta catactcttt atcatttata ataagttttg
     6001 aacctgcttt aatttctata tctggagata taaagagttt aatagtttgc tctagcttag
     6061 ctaatttccc ttctgtagca gaaactatat ttttatatga aagtttacat ggttgatttt
     6121 ctaatacaat cacttcttta ttgttagttc gttttgttac agggtcttta attggctgat
     6181 actcaactat agtacattta tctctatata acatttctat tgcttttcta gttttactta
     6241 ccatcttaag cacctaaagg ttaatatctt attcttacca taagtagtaa gataagctat
     6301 taagctatca aagcgttgtt ctggtgtttg agaaccactt cctatagcaa aatctacctt
     6361 tgtatcacct tctgatatag acttttctac agcttcaaag ttaatgcttt ctatatctaa
     6421 ttgacccata tttttcttgg taaataagaa ctctccaact atcatatcaa cttcaatttc
     6481 tttcaattca attggcatag tttttatatt acaatctagt ttaataatat tttctatttt
     6541 ttctcttaca aaacctatta accacttatc tccatctttt aatatatatc caaaactttc
     6601 aagtcttttt tctatttcat caattatatt attttccata attttcacct actttttagt
     6661 aagtttattt ttctctttaa gctgcttatt ttcttcttct aaagactcaa cttttgacct
     6721 taaaatatta ttttcagcta ttaaatcttt tacacttaat gacttgccat actttactgc
     6781 cttaccagtt tcatctatca aatcatatcc catctctaag aaatcatcta ttttacattc
     6841 ttctatggtt agtattctat ttagtttttt tacttgtggc actatatatc actccttttt
     6901 ctatgcttca acaacaaatt gtattgcatc agctttttta tttaatataa atacatcctc
     6961 aaaactttct tcaaagtaga agtattttcc ctctgtaact gctgttggtt cgtctaactt
     7021 agagaactga taagaaacag gtgtaattat tgcacttggg tgaactaagg acataaagat
     7081 ttgtttagct cctgctccta ctttccatcc agttgtaaaa tcatatgcag ttttcattag
     7141 attagatggt actttaatta ttttaactgt gtcaatatct gtggtttgtc tatttaaaga
     7201 agttccacca tcttttatgt ttactgttct ttgtatctct tttgcatttt tgataagtgt
     7261 atttactact ggagtaacat acaatattct tccattttca ggtactctag cttctgtcat
     7321 tttttccatt aacttatcaa atacttctaa tacgtttgct gttgtaagaa cagttgtatc
     7381 tgctgtgtta cctaatgcgg tccaatcagc atatatttta gatatacagt aagcatccat
     7441 ctctggaaac ttttgttcct cattatatac ttttgttata ttacctattg aagccacata
     7501 attagtttgg tttatatctg ctggatgaac caatgttgac catttccttt gattagttaa
     7561 taccttaggt tcccaagcat tatcatagtt tctttgagct actgctattg tatctctgtt
     7621 tgaatctacc cttccagttg tagatatagt tggtatttct attgttttag aacccgtcca
     7681 tctatatctt ccattatttt ctgttgcata caaatccccg aagtttaaag tataaggata
     7741 tgcttgtgct aaaacatttg aatattcttt tgcataattt agtgctgcca ttttatttcc
     7801 tcctatttat tattattttc atgaggtctt accccagtaa aattaaaacc aaaatcattt
     7861 atcttaggct cttgccctgg tgttatagta tctattttag gttcttcacc ttctagtgtt
     7921 gcattaaaca aataatcttt atcctgtttc aaagggttta tttgctcttc aaaagctttt
     7981 tgtctatctt tactatttct tagggcttcc atgtctaaat gagcttttaa cgctatttca
     8041 tctctacatt taacagattt aaaagcatca cctaaccagt aattaaaatc tttttcttca
     8101 atttcttttt tgtaggtttc ttccaaagtt ttcttatcag tttcataagt tgtttttaga
     8161 ttctctacat cttcttttgt catacctcct tcaaactttt taatagtttc attagctgta
     8221 ttaagttgtg tttcaagatt tacataatct tcttgagtaa ctgtagtctc ctttattttc
     8281 ttttctatag atttttgaag agaagctaca tcaattttgt tatcctctac ttttattcct
     8341 tctagcaatt cttttaacca atccatttta aatttctcct ttcatttttt acaaaataaa
     8401 agcacctact aatttattat ttagcaagtg cttttacata tttactattt gtatatcttt
     8461 ccataaatcc tttagtaatt taccatcaat gttgtaatta tcaaccatat ccttaccatt
     8521 tttataatac tttgtatctc cattagggca aagagtaata aaccttgtat catcatctcc
     8581 aatagatata ttatatggtt tattatataa gtcaaattct atatctaatc ctaaatcaat
     8641 agaatcaatt aaatgttgta aattcttaaa tttattatcc attttattct ccattcaaaa
     8701 tatctttgtt agctatttta tgagcttttg taagctccat atccttctct ctttttacct
     8761 tatcatggtt attttcatca gctaaccaat catgtttatg aggtacaatt ttatgttgct
     8821 ttgggtttct atggtcagtt aagtctaaat ctaatctagg ttttcctgta ttaccatagt
     8881 atcttctttg aattaattta ccatctttgt aattatcaaa tacactattt ggttttgatt
     8941 caaatggcac tgaatgaaca cttccactag ttaaatttct ctgattctta acttgccaat
     9001 ttacatcctt ataaagcttt ttagcttcct cataccttat agtatcatta tacttcatat
     9061 gttgatattc atcaaattta gaaggtattt catttcctaa taccttttta tattcttcaa
     9121 attgttttct atctttactc tcatttagtt gcatttttct aagggtgtca gctttttctt
     9181 ttccaagtct accctctata tgtttctcat accactcatt atacttcata ctagatggta
     9241 cataatatgt ttttccatct tctcctcttg ctgctctata gccttcttca tcctcgaacc
     9301 aaggagctgt tgttgtccta caacgacaat gaaatggtgg agctgttatt ccaacttgat
     9361 aatctttcat atcaaatatt tttccatcta actctctgca tatatttgaa gttcttaaat
     9421 ctaatgtagc aataatctca tatttctcta catctaaatc actaaaacaa tcttttctac
     9481 ttgctgatgc aaagaaagct gattcagtca ttatcaaatt cttagcttgt gatttagata
     9541 cattaaatct cttagcaaag tcatttacta gattttttgg attttcacct ctaataattg
     9601 attgagttag cttagtatgt agttcattta ttaaagtagg tctatgctta ccccaaattc
     9661 tttcactaaa gtttaatcca tctgttgccc atggtttaga gataatttta tttattctat
     9721 tagtatcaag actcattaaa ctccaaccaa cgtttactcc ttgttgaaca ttaaaagctg
     9781 tatgatagta tccacttgta taaatatctc tcattagttt atcaatacca tcaagttcat
     9841 ttccatataa aacttctacg tgttgctgta tttgtaactt taaagcttca agccttgtta
     9901 tatgaagtct tgcactagca ttttctaact ccttcatcca cttttgattt atagcatttt
     9961 ctttgccata tttaatatac tcttctacat tccacttaaa ttcttctaat tctcttgtat
    10021 ttaacagttt cttagcttct aataaagata ttccttcatt ttcagcaaat ctgttatacc
    10081 atgataatat atctttttct atactattca tagctaattt gtatttcttt tccaattcaa
    10141 gataatattt tacactttta ttgttttgaa cttcttctaa ttgttcaaat cttttcctcc
    10201 aataatcttt atgtttcatc tataacaccc tcttgattat tagtaggaat taaatcatca
    10261 tattcttttt gggtttcttc ctgtttttta agtctttcaa gttcgtcatt aacatcttca
    10321 acccaaggat gattactaac aatagtttca tctgatataa ttccaattga tttagaagcc
    10381 atatctattt tttcagactc atttattatc atagaatgat taaatgttat ttgaactgac
    10441 ttataatcat agctcttact accacttatc tttaaatact cacacacaaa ccataaaagc
    10501 tctctaattg cttttttaaa cttcttttca gtcttggaac attttaaatc aagtagtgaa
    10561 tataaaaatt taagagctac acccgatttg tcacctgtgt tttgagattc gggattaact
    10621 ccttgaccaa aaataattat attcttttct aatctatcaa gaagctcctt tttggcttca
    10681 actggtatat ttatctctag tttatcaact ccacctccac catctacttt aattgattta
    10741 tagtatctta tattatctat aaactcttgt agacttgttc ctggatactc ttttaataca
    10801 taaatgacct cttgcatttc atctaaattg tctgcaagtg tagaaatgtt attatcatat
    10861 atatcaatta aagacttata gaaagttaaa tctgagacac acttttcatt atttttaaaa
    10921 ggtataaatg gaactttacc ccatccctgt tctttgttat ttattctaaa atgaccttct
    10981 tgtatatcag tcatttttcc atattcatca tataaaaatt cttgaataaa actattacct
    11041 ctttcaataa agtaagttac gtcattttct gtgtagtact caactctttt tattttattt
    11101 ccatctatat cttcaatata ataaaaccta ataaatgcaa ctaattccct ctgtctttta
    11161 ctatcccaaa taggaattgc ttcttcagct ggaattatta catatttaaa ctcacctttt
    11221 ctattaatat atggatgtaa ccattcaacc cctttattac tagcattgag atatagttct
    11281 gttattgtat cgtcaaactc ttctcctagt aagtcattta aaagcttagt gagattatca
    11341 tcatctgcat taaatactat gggatttccg acactatagc ctaccttttg gtcaactaaa
    11401 agcttatggt agttgttaat tgctttatta ttaactttag taaaatcatc aaccttagct
    11461 ccatctaaga gataatatct tctcttattg tttacatcag tattaccata atagtattct
    11521 tctccttgtt tatatttttc tggcctatgc tttaagatgt agtgttctat gacttttact
    11581 aggttaaagg tgctctcttt ttttaactga acttttatta aatctgtttc acttatataa
    11641 atatttaaca cctcctttac tttaagaagc ttattccatt atttttaagc ttattatcta
    11701 tagaatatct aagagcagcc attgcatcat ccataaactc aactggttca tcaagatata
    11761 atccagttct ttcgtcttgt ttccatttcc attgttgtat ttcttttatg gtattagtgc
    11821 aactaggatg tacatgtatt cttaattgtt tcaaataatc tatttgagct ttaacacttc
    11881 ctggtccttt tttaactcct ttagctttat atcctgcact cttccacatc ttaattctat
    11941 ctggttcagc actatcacag tacataaata gagtcttttc taaacctcta ctatttgcaa
    12001 tttttatgat ttctgaggta tccatttcat gtacatatat ttcattacat atatataact
    12061 ctccatcctt aaagccaatt ctaagtattg catttgcatg attaaagcca aagtcttgtg
    12121 acagtctcat attgtcaaag tattcaaatt ctgtgggaaa ttcatgtata acataatttt
    12181 taagtattgc tccaccagtt tctccccatt ctccaagacc atatactttg tacccttctg
    12241 ggtcttgctc ttttctcatt tgcattcttc tgtagtaagc ttcatctatg aatctatttt
    12301 gtagataagt actatgatga gtaaatatat catcattttt atagtcaaaa tactttcttt
    12361 ttatccaatg agtagctgag actggattaa atgtaaatgt catttgataa tacaagttag
    12421 gattagttaa aatacctctt aaacggtcat ctagtatatc tatgtcactt tccataagtt
    12481 ctgtagcttc ttcacaccaa acccatgtta attttccttt cgagaagtta attgatttta
    12541 atttttctct ttgttttgca tcattaactc ctctgaaaat tatagagtta ccagtaactt
    12601 tactcttaat ttctaaagga tttaaagtag ttttccaata cttatcagct tgtttaccat
    12661 aaatacgatt tatagctcct gtaagctctg catacgttga atacttatgt gtagcttctg
    12721 actttctaac tactaataga ttagctcctt gatacttctt atctcctaac tttagtatat
    12781 agtcttgtgc tacattaaca gattttccac tccctgctga acctttcatt gctctgtatc
    12841 tttttttagt aaaattagct tccttgaaat ctggattaaa atttattcta actatcattt
    12901 ctatcaccat aatctacact tattttcaac tcatcatctc caatatcatc tttatttagg
    12961 ttagcaacct cacatttcaa cttctcaact cttgttttct gctcctctgt agccaaattc
    13021 caatccttat gaatcatttc atcatactgc ttaattaaac tccttaactc actcatagct
    13081 ctactctgtg cattaagaaa agatgcttgc ctatcccatg caaattgaaa ttcatactct
    13141 atcttctcac tattttctgt gatttcatgt ttctttaatt ccttaatcat ttcctcttta
    13201 tctttaacat acattatctt ctgtgctctt attattgctg cgtattgaat tgttatctgt
    13261 tcccaaagaa tatcaaattt atcttttata gatatttctt gtattaattc cctagtttct
    13321 tcaggtaagt attttgagaa gaaaccaaac ttttcagcat ttttattctc ttttggagca
    13381 ccataaccaa ctgaattttt attagaaaag ggtgcacccc ttttatttat aggtgcaccc
    13441 ttcttttttt cactagacca attgtatctt tttatccatg actttaaagt atttaaacta
    13501 atgtcatact ttgctgatat ttccttttgt ttcattcctt ttaagtaatc ttgttttacc
    13561 ttttctttga catcttgcac atcaccacct ctttatttgt ttgttttggg aataaaaaaa
    13621 caatcctatt taataggatt gtttcaaatc tagtctcctc tatccttcca ggataaatga
    13681 aacatactta ttattaatgt agttagagat aaaattatat gaactgtaaa aaatattaaa
    13741 acatagaaaa ttatattaga ataatatcga gtaagcatta ttgtattgat aaaacatatt
    13801 acaacaacca aaatctccat tattacagta taatatgctt ttaactgtat gaattgaaag
    13861 tatgatatat taaatccatt tacctttctt tctgttttta tatcttttgc ttctgatatt
    13921 gatttgttat atgtagtaaa caataatgta ataatgcaaa ctccaaaagc tactaatagt
    13981 cctgacaaag ttagtacatc tttgtttaaa gaaattacaa cttctttatt aattgacgaa
    14041 ttaaaaaaca tgtatgaaat aatacatatt aatactggtg ttaaaactaa ctctactact
    14101 tctttagaat ttatagcctt gtaataattg agtaagatat ttaatacctt tttatacatt
    14161 ttaacacctc cttagcaatc tatgcttcac tcatagcaac ttcttgatac attaaattaa
    14221 aaaaaactga attattctca attaaatcat ctatcaaatt attcatcttc tcaaaaatgt
    14281 cttcactatc tatatgatta tctatatcaa gagaaacctc tatttctcta cacaacttta
    14341 taccctcagt atccaacttt aattttctct tatttttctc tccctcaatt attatcctat
    14401 ttattttttt atttctaaat tcctctcctt taatatattt ttcgcaacat cttttaactt
    14461 cactaggtaa atatctagtt ctaaatgtgg gcttatatat caaatcgtta tgtcgtcttg
    14521 aattatttaa attagaaaac aaattatctt catcagttcc aaacttctct ctatcaacat
    14581 ttaaagttat ttttgaaaca ccatctaaat ctaatatttg ttctataaaa tctttacttg
    14641 ctatttgtgt aatattaatt gatgtttttg acatattatt atcaaattta ttattttcaa
    14701 tcatatcctc ataatataga tgtaagtttt tacttagttc aatcatggga acccccacaa
    14761 aaactctttc aaataatact actgctatat tattttcctt cataaattta ataaataggt
    14821 gctgcttatt gctatctcct tgatccattt ttttattacc ctctacttct ttagtatgag
    14881 catttataat atttgtattc tgattgtatt tagtatattc aagtttcacc ttgattatta
    14941 aatcatcctc gctacatttt acttctgtaa tccaaaactt tttatcttta gtaatattaa
    15001 ttccattttt gttttcttta caatattctt tatccttaaa gaaggtcttt attacctctt
    15061 ttaaaatttc ttcatctgaa tagacactcc cattaatttt atttataata tctattctat
    15121 aaacatcaat atatcttttt ttaaaattac tcattatacc attattcctt tcacaaatat
    15181 ttataatgat ataattctat ataatcagtc aatatccttc aataatcatt cgacaaccac
    15241 aaaataagtc taaataatac ttttacctta ttcctgtcta taatgtacaa atatatttca
    15301 tttttaattc attacataaa aaaaaagacc ctctattaat acagtcattt aaatcatttc
    15361 gattaattct ttaatctttt tatatacctc tttataattc atacctttat ctatcaacct
    15421 aggtaatttc atagatataa ctctttcaag tacttgtatt aataaaaaaa ctcttgtatt
    15481 tcatctgtaa aatcttttct gaccttttac cttctgttct tatatccagc cattcttgat
    15541 ctttttctgt ggcaatatag taaccatgta ttctaatttg tttaagtgct tttgtaaccc
    15601 acttcgtaaa tagttttgct tctgctttat tacttctaaa tgacatattg tacacagctt
    15661 cttctgtaac aaaagtagta ccaaagttag gcaatttatc tttaaagttt ctagtgtagg
    15721 aatctccgac agtagactca ttaaatttct ttttatattc tctatctata tttcttaatg
    15781 tatcacgaat atttactatg cctagttcct ctcctacgtc atttgcatta aaccaaactt
    15841 cttctccatt ttttgaccat atcatcctta catttttctc ttgtaaaatt ttcaacatac
    15901 tactacctcc taaattagtt attaataaag aatattcaat ttgaacaccc ttattttcga
    15961 cccttcaata tgagagatta aaattgtata aaaaaagacc tagaaatcac tctaagtcat
    16021 gaattactca tttatcattc tccttattta caaaataaaa aagactaagt tggggtaact
    16081 tagccttttt caagggggaa tatattatac acttgtttca tactaccatt ataacagggg
    16141 ttaaatatca ttaaaatatc atctttttat catgtattta tcaaacagtt aatttcaagc
    16201 catctatacc aaataaatac actccaagtt cttttaccat ttcattaatc caacgtcttg
    16261 atgtaactac tccacaattt aatatctctg ctatttcttc atatgttttt tcatcacaat
    16321 agtaaagctc taaagctcta tacttttcat aagaatgtaa acgctcttgt cttaatttca
    16381 aagttttcat tgctgaatct atatgtgcta tcataataat agttcttgct ttacttttct
    16441 ttatacttaa aatatacaac tcttcattat caagcttacc taaatcatcc tctaagtaat
    16501 ctacatcttt agcttcactt accgctgagt ctatatgtct cttaaaatca ttataatgct
    16561 tcatcaataa tctagtatta tataaaacgc cttttttcct atcttctctt tcttcttttc
    16621 ttaactctct tacaatttct tttatacttt ctttatccac ttaaatcact ccttcttgct
    16681 ctcttcgaac aacttctact gcaatactca actttagatt tatccaactt ataaaatctt
    16741 ctaccacacc aagcacatct tactatttta cctgggctta taagttccat cttttctttt
    16801 tactttcata ccttcactcc ctatattctt ttaattttgc ttttactgct tcaagtaatg
    16861 cactttgccc tttatcctta ttctctatag cttccattac ttgttcatct attgtacctt
    16921 tacagattag atgatggata ataacagttt ccctttgacc ctgtctataa agccttgcat
    16981 tagcttgttg atacagttct aagctccaag ttaatccaaa ccaaacaatt atacttccac
    17041 ctgattgtaa attaagtcca tgtcctgtac ttgctggatg acaaagtaat aattgtattc
    17101 ttccattatt ccaatcatat atatcttttg agttttctat tgttcttggt ttcaaactct
    17161 taaactcttt cattaaacga ttataatcat gtttatagtt ataaaaaact ataataggct
    17221 taccatttga aacatctata atttctttta aagcttctaa tttttctcta tggagttcct
    17281 ttacattttt atcattatca taaattgctc cattagctaa ttgtaaaagt ttatttgctg
    17341 ctacagctgc tgatgaagct gttattatat ctctatctaa ttctaatatc ttctcttttt
    17401 ctaattcttt gtaatattta agtattttac tatcaaggtg tatttcaatt ttattatcta
    17461 tttttctagg catattcaaa taatcttctg cttttaagct tatacaaata tcttttattt
    17521 tattatgtat tgcattttct gctccatttt taggttgcca attatatata gctcctgttt
    17581 gataattttt ttgtcctggc tcaaagtatc gttctttata tcctgtaata gttttaccta
    17641 gtctttcacc tctatcaagt aagtacatct gtgcccataa gtctattaaa ctatttggtg
    17701 ttggtgtacc agtaagccca actactcttt tagttaaagg caatactttc tttaaacttt
    17761 taaatcgttg tgctttattt gatttaaaag aactaagttc atctatcaca accatatcaa
    17821 atggccattt tcttttataa aaatccacta tccagggtac catttctcta tttattatat
    17881 aaatatctga gtctgtactt aaagctctca ctctatctaa tttactacca agaactttag
    17941 atacttttag atgctttaga tgttcccatt tttttacttc actactccag gtatctcttg
    18001 caactcttaa aggtgctata actaaaactt ttgatatatc aaagtaatca tacattaatt
    18061 cacttatagc tgttagagta cagactgttt tacctaatcc catatctaat agtaaaccta
    18121 tattattatt atctatagtt tttctaattg tgtattcttg gtatggatgt ggtttaaatt
    18181 ccatccctta acctccttga taaaatcatc tatctctttt aatgtgctta cacacttaac
    18241 tttaaatcct aattctctta attctcgcat tttatattgt tgaagcttcc ttagtttttt
    18301 acctggtgct ttaagttcta caaatataac atgtccttct ggtaataaga caatcctatc
    18361 tggcacacct gcctcacctg gtgaaataaa cttcatagcc tttccaccta acaactcaat
    18421 ctctttttta agtctttttt ctatttttga ttctaacaaa atatcacttc cttaaaattt
    18481 aagtgtcaac aatgtcaaca gattttctat atatatgtat atatacatat taggcataca
    18541 tatgtatata cgtatatgcc taatattact tatactactt tatacttaat ttttgttgac
    18601 attgttgaca ttaatacctc aaacattgta attactgcat cttaggtgtc aacataagcg
    18661 tcaacaaagc catttttttt gttgacattg ttgacacttt taaaattttc aaatttactc
    18721 tgtttttaat tctgttgaca ctaactttta cgtacataag ctctctgcct accatatatt
    18781 ttcccaaacc ttaaatttcc attatatgat atccaatcat ctaatccttt taatatatca
    18841 ttaatttctc ttgataagat aggcgtgagt tgtttaggtt ctccattaaa tagctcaacc
    18901 catatctcca taacacacgt tttttctctt aatattgttc cttcttttaa atcaccaaaa
    18961 tctgaaccat gaatatattc tctcttttct gaaatactta aatcatacca attctcagta
    19021 ataggtttat ttagatactc ttcgataatt ccagatttag cattttcttc tgagtgagtt
    19081 ctttgttgtc tttcagcttc ttttttctcc tcatctgata gatatagctg ttcattggct
    19141 ttacataatt ctactgcttc tgcccaaatt tgatttcttt cattatcaag ttgaccatta
    19201 aatatactct tgttaacctt ctttactcct gtatctattg gccaaaacct tctattcccg
    19261 gtcttatctc ttaaaaattc tttatcatta gttgttccta taactacaca ttgacgtaaa
    19321 aaccttgatg ttctcttccc atatgcgacc ctatatatat cctctgattt gcttagaaaa
    19381 tgcttaactg cctcaatatc tgctttttta gtagccatca tttcacccat ttccagcaac
    19441 catactcctt gtaattgttc atacgcttct ttaccttgta cagtagttaa actatcagaa
    19501 taccagtctc caccaagttt tttaataaaa gtactttttc ccattccttg aggaccagat
    19561 aaaaccatca tattatcaaa ctttattcct ggattaaata ctcttgctac tgctgctact
    19621 aaaacttttc ttattatagt ccttgtatag tggttatctt ctgcacctaa atagtcaatt
    19681 aaaagtgtat ccactctctt aatgccatcc cactttaaag aatttaaata atcttttata
    19741 ggatgaaaag tattattttc aaaagcaatt attagagcat catttacttt tgatggtgat
    19801 gagatattgt aaattgtttc tatatgatgt ctaagcccag aatcatcact atcattccaa
    19861 tcatttaatt tatcatcttt tctccaaggt aacttaccta aaacgacagc tctatttgaa
    19921 aattcattat aagctatttt tcctttcaaa tatgggtcat tttctataaa cattaagata
    19981 ttgtttgttg ttttcttata acttccctta ttgtcataat ctaacctagt taaccattca
    20041 tcatcttcaa aatctatatc accaaaatca tccttagctt tatcaaggtt ttctcttcct
    20101 atagtctttc gtactttagt gtcactgctt gcaaattcgc tcattcgagt aaatgaaggt
    20161 aatctattta caggcgtttc gggtttagca tcttcatcaa gttcaccaaa tttatgtatc
    20221 ctaactaaat caaaagcatt gcataaaatt ccgcttgctg ggtctgtacc atgatgacta
    20281 tatgaaaact tgtcatcata aataactact ccaccacttg tactaccttc tgaatatgta
    20341 tacctggttt catcaatacc aggaatatat acttcattta agaaattttc tatagtttct
    20401 tttatactat aggacctgca aaatgcacct ataattccag ccttttcaat agggtcttgt
    20461 tgttttttta attgtgtatt aaacttttgc ctctccctcg aactttctgg ccaatagctt
    20521 acatctgtcc agtctaaata taaatctagt atttcatttg ggtttaaaaa ctctccatct
    20581 tgaaatttaa atatatagtc tccatcaatt gaagtacttg gaaaatacat aagtctatga
    20641 ggttgatatg tagtatcatc aaacatatct attcctatag tatctgctat catcctggaa
    20701 attgcttgat attcttctgg tagtactggt ctagttaaag gtattacaag tctatatctg
    20761 ggattattct cagtatgtga gtgcgtagaa tacataagac aagcataatc atttaataat
    20821 gttatatcct cccatatatc tttattcgca taatctatat ctaaagttat aatacttcta
    20881 tttgcaatat tctctgcttt tcgtcttcca ttttttaagc ttccacctac aaatccacca
    20941 acatctttaa ctctatcttt ttcagtcttt gacatcttcc tatattctgt atatgtttct
    21001 tgagttctta atgttttact taatctattt acaagttcgg accataaaat acttttattt
    21061 ttccaatgtg tttctaattt attttttcct atggccagca tgagttggcc atcatgtctt
    21121 acatttatgt gttcaatttc actggccttt atatccataa atcaatcacc tagtctttct
    21181 tataataatc acattcatat ccatctgctt taagaggaag acctttagcc caagatattt
    21241 cttttcccat aatactgtta acttcttcta aagaacctcc ttttttatct acatcaatta
    21301 caagctcatc atgtacatgc attacaatgc tataacctgc atctgtcaca ttaaacatag
    21361 cctctcttaa gcaatctcta gctgtagctt gaacaatatt ctcaactaac ttaggtccat
    21421 aagtatctat tcttttccat tgtttacttg tctgttccat accttcatat gttatcttat
    21481 ccccactaaa tgtagtatga ggctctatct taggtcttag atatgataat cttctaccac
    21541 ttggtagttc tataaataaa actcctggat tataaataaa tttaatccca tgttggagtt
    21601 ttactatagt cctatctttt atggcttttt tagctgcctt atctacatcc caccaaaatt
    21661 tagttatatt tggattagca tttctccatg ttgttacaag aggttgaagc tcctcttcat
    21721 caagacccat ttttatagcc cccattgatg ttaaagctcc tatgcttccc ccatatccaa
    21781 gagctagctc tgaaatctta cctttttgtc taagtgggtc acctttttta atactttcta
    21841 ttggaacttt aaacatatga ctagcactag cttcatatat tttcccgtga gaattaaata
    21901 catctaatct ccacttctca ccagcgaacc aagctataac cctagcttct attgcactaa
    21961 aatctgatac tataaatcta tgaccttcac ttggtataaa agctgtcctt atcaattgac
    22021 ttaagacatc tggtacacta tcatataaaa gctctattaa atcaaaatct ccttctttta
    22081 ataggtttct agctaggtct aaatcctcta tatgattttg tggtaaattc tgtacttgta
    22141 ctaatcttcc tgcccatcta ccagtcctat tagccccata aaactgtagt agacctctta
    22201 ctctattgtc attaccttta gctaatttca tagcctcata tttctttata gaagttttgg
    22261 acattaattt tctaagttct aaaattctaa ctacattttc atcatcaact tgttttaata
    22321 tttctggaat actttctttt gttaggcttg taatctcaaa gccaacttta tcacttaacc
    22381 atttttttaa ttgagctgga ctatttggat tatttagacc agttatttta attgcttctt
    22441 ttgtaagttt ttcagtatat cttttatcac attctattgc attctctatt aactctgtat
    22501 caactttaat accagtatca ttaattctct ggtctaaata ccataattta atttctcttt
    22561 cggtagtctt atacttacta agtttgtttc ttatttctct ttcaactaca acatcttgtt
    22621 tacaatattc tttaaattta ttccattttt ccatatcatg tattggtaaa tttctagttc
    22681 ttcccttgtt aacttttgta gccttacaag gtttacagaa atattgtatt aatgctttac
    22741 cttctttcat tttctgttta tcttcattaa actttaaagc cttagacaca ctatctaaac
    22801 ttcctggaag tcctagtgtt aacgccttta tcattgtaca tgaccactca tttggtttta
    22861 aattaatatt taaaaattta cttattgctg ttctttcaaa attagcatta aatgctgatt
    22921 ttataacttt attatcattt aaagcttcta ttacttcttt tggtaactct tcatcattta
    22981 ctaaatcaat aacttttacc tcttcattat caaaagcata ggcaaataac agtatctcaa
    23041 aattagcaga gtctacatat ctgtagactc caactttttt tatatctaaa tcactatatg
    23101 tttctatatc aattgataag gtcctcatta acttaagaag tcctcttctt catcttcata
    23161 ttcaaagtca tctgagaaat ctgcttctgc actagctcta gctcctccaa gtacttctcc
    23221 atctgctaac ttttgaacat tttgtaagcc acaacctatt cctttatttc ctgcactatt
    23281 atatgggaaa aagtttatac taactcttcc ataacaacca ctatatacct ctgtattatc
    23341 taatatttca tttaagtctt tatcaactat tcctggcttt tgtgtactat ttgcatttaa
    23401 aaaatacatt cctacatatt cctctgcttc atcagctctt tctgcatctc catcacgaag
    23461 aggtgttttt aaattacctg gtaattttcc accccattta gaagttttac cttgttcttt
    23521 agctgcatca atagccttct ttattcttcc taaagtcacc ttatctgatt ttggtattaa
    23581 aatacaaact gaatacttgg gctctgcacc ttctaccatt gctctgcttt taaagatatt
    23641 gcaataactt aatcttacct ttcctgttac tacctttgtt gattgtactg aattactcat
    23701 aattttcttc ctcctaatat ttttatattt ggtatattga ttttatattt gaagttattt
    23761 ctccaaattt atctaaatct ctaacttgct tactaaacaa tttaattctt ccacaaataa
    23821 ctgttgaaga aacatttaat aactctccta tatcagatat ctttttatct tgtaaaatcc
    23881 aatctactat tttatcaata tcttttaaat atgactttct tgcaattttt cttatataat
    23941 caacatcata gttaaattga atttcataaa atccatgtat atcttttaat ggtcttttac
    24001 tatcttcaaa tacacttaca tctaaacctt ctatagagtt tctataatgt ttttcgccat
    24061 aacgcaaagc tttaaacatt tctattctta tgtaagaaac agccactgta gaaaacttgc
    24121 ctctgttact atcataattt aatgctgctt tatataaccc aatacatcct tcttgataaa
    24181 actcttcata taagtatgga tgttgctcta cataagactt aaaaaatcta tttatactaa
    24241 aatgtacaag attcaaatta ttttcaacta gtttagttat ttcttgatta ctcataaaat
    24301 tatatcccct tttaaatctt tttatagcta ctaggaatta aatattttta attatccttt
    24361 gctcttactg atacaaagca tactggacta acttttatat gttctggtat atacactcta
    24421 tcaagatgat tgtcatttac ttcgaggaaa gttgacatca ctttatcctt tattaaactt
    24481 tctcttttaa aatccatcac tataactttt gcattttctt cagttgagtt atattctgta
    24541 atatttagtc tctttaacat atctagtaaa ttatcttttt cataatctaa atctttttcg
    24601 atactctttt ttctatcctt taaatctaaa attatactta tgctttcatc taatttatct
    24661 tttatacctg catctaatgg catgtaatat cccccattta tatttcaaaa tcttttttcg
    24721 ctgaatctat actattaatt tctgaccttt tatcactttc tactactaaa gtagcttttc
    24781 caactggttt tataattaaa tcacttagta gtttagcaaa actcttttta cctatagctt
    24841 tctccatatc actaattcct tttaaggtcc ttggcttata aattttttct tcatcataat
    24901 ctgaatttaa taaaacctta gcaacttctt gctcatctac atactttcta ttacttctgc
    24961 cttctacaag cttatatcct ggatatttaa caccatgctt ttcagcttgt tctagtgcat
    25021 aactttgaac atcctttaac caatcttgta tattcttagc aaaacctaaa atatctgcta
    25081 tctcatattt attaagagca aatgtgtcag caaaatcata ttttctagct agtttaaggt
    25141 tatcctcagc tctttttctg caatcatttt tagctctaca aaatccacaa tgactgccac
    25201 ttacaaactc tccttcacca ttaaaagcca tttgagcctt cttcttaaca ttatctgccc
    25261 atgtaagtaa tttagtaact tctatttctt cacttgatat gttatcaagt ctaggttgaa
    25321 ttattgttgt tttaattaaa tcaatatcgt atagcatttc aaactgatta tatgccccta
    25381 aaccatatag tctaagttga ggattctcta tagctgaaac ttctaaacct tttccatact
    25441 ttaggtctat aacttgaagt ataccatcag atattacaac aacgtctcct gttccaaatc
    25501 cttctggaac ccactcacta aaatcgagtc tttcttctaa catcactatt acatcatcac
    25561 atatagcttt actatcattt actaattcaa ctacattctc aacataagat tgtatatagt
    25621 cctccatttc agagttataa tactcacttt tctgtatctt tttaattctt gcattatatg
    25681 cctttttact tattttttca tactctagca ttaatttaac ttctgctaat tcatgtgcaa
    25741 ctgttccttc ttctgcatat atactagtta atggtggata gttttcttct aattttatac
    25801 taggagtgca gtgaagccat ctatgggctc cactcgcact aagtcttgca tgttgtaatg
    25861 gcatttatgt accccctata aactttctaa tttgttcata aaagctgaat aatcttcttc
    25921 ttttacttca cttaacttac tagctccaaa ttcaccaaat aactccttaa gcttatcctt
    25981 cttacctgcc ttacttactt gtgctgcctt agttcttacc tcttcttttg tgtatttaac
    26041 ttcactagtt gtattttcat tattcttttc tatttcttcc tttgcttctg ctatttcttc
    26101 tttaacgtct tcttttactt ctaccttttt aatcttttca gcttttttaa cttcaacttt
    26161 cttctcttct ttcatatctg ttatatttat aggttctatc tgcattgctt ttcctagatt
    26221 aagacctcct aaagcattcg ccactactaa tagtgcattt gtaaattctg gtgcttctac
    26281 tttaactttt acatttacat taacttcaac cattttaaat atctcctttt tgtgttatac
    26341 tttacttgtg ttatatttta ttttttattt ttttgtgtgt tggttatttt accaacactt
    26401 ttttatttaa tattccaacc gatattttct taccagtttt aatatcttta aatactatat
    26461 ctgctataac ttttccatct tttttaagag ttactacatt cttattatta gtatcaagac
    26521 tcagcaattt catcccccca ctctactgct tttaaaagtt catccaaatt tttaccttga
    26581 tttctttcaa taaaatcatc aacttcatat cttgaaattt ttctaccatc acctctaact
    26641 agtgatttta tcaaacctgt gcttactaac ctacgcataa aagctgtatc taactttaaa
    26701 attccccttg cttcttctac tgttattaga taatttggat aacctctttt tattaaaaca
    26761 actatatctt taggctccag tacctttacc ttttgttcaa tagtttgact cttaactcta
    26821 tctgtctctt gtttacttat ctctatttct attaaacttt ttaaactatc actaaattgt
    26881 tttgctatac tctcagaaat atccatgtga aaattctctc ccctcttttt aattatttat
    26941 cttccaacta attcgtctaa tgtaatatct aaataatcag ctaattttat tagagtatct
    27001 atagttggat tagaactttt atttctcagt aatttatata aaccacttac atctatattc
    27061 aattcttttg ccaatttata aggttttata tctctttttc ttagcatttg ctttatattg
    27121 tcgcctatta acattgtatt cacctcagtt ctacatggta taatataaac atggaataaa
    27181 ttccaaaatt ttcaaaagga tggtgtatta tgcagttttc aaacaatata tctaaagaat
    27241 tacaagaaag tataagaaag acagtctctg atacttctaa cacagagtat ttttactatg
    27301 ctgaatttca atataaaatt attcttaaat ctatagagga atttgaaaaa gaacttgatg
    27361 atgaacatga aattgcacta aaattgacta actttggaaa agatgtctta atgattgttg
    27421 aagaagtagg ttatcataac ccttgtctaa ttcactacta cggcatagta aatggagttt
    27481 attctgaaat tcttcaacac acatcacaga taaactttat gataacttca gtaaagaaaa
    27541 ctgacccttc aaaacctgct agaagaatag gttttatatt gtaattatca ttcttatttt
    27601 ctgattcttt ctttagtaat ttcactaatt tttctttttt cacttttatt catctcatgt
    27661 aaatacaaaa ctatacattg gtacatataa tctataaaat cactatttga ttgagctgtt
    27721 atctgactaa ataactgtgc ttgcaattga ttttcaagca tggttattct tttctctatt
    27781 tcttctattt tcttattttc caattcaatc tctcctttct aaaatattct gtatttagtt
    27841 ttcaaagtac taacctaata ttgaagtttg ataactatat cctttttcta tatctggtaa
    27901 tatgttattt gttttcaata aatcatataa aaacaatctt cccttttgag tccacttagt
    27961 tgtcattttt acatcaggca ttccattact tctagttatg tctattgttt ctgaatgagt
    28021 gtatcccttc ccttgatgtt gtttatataa aagccattgt ccactttgtt tatattgaat
    28081 ccctctttca tgaagtattt tattcatttc ttttccactc attccatagt cttttgctat
    28141 ttgagttatt gtgacaagtc ctttattttt taatatcata tctgtataat ctgcctttgg
    28201 ttttagttct tttattactt ggtctttcat tttactttct agttgtaact gctcatttac
    28261 ttctacttgc tctataagat gttgtaacgc ttctttatat gtagttggta gtttagtttg
    28321 caaatctatc atattatatg tcccagtact acgtatactt ggtaatatct cattagttac
    28381 ccaacgttta aataattttg cacttggcaa cttagaactt aatattaaag aataaagacc
    28441 actttcatta attactttca tattttgatt accaccagga gtcggtattt cacctactcc
    28501 tttatcttca tcatcaacat gtcttttcaa agcatcagat gtatctttat atcctaattg
    28561 ttctgcaata tcttttccaa caaaccaaaa ttctccattt agttcgatta ctcttatttc
    28621 tccaaaagtc ttatttttga atatttgtaa attattcata atttctttcc tccttatgat
    28681 tgtttttgtg aacaataaat tttaatttat gttaaccttt tttctaaaaa aagttcatcc
    28741 attgttttat ttaaaatata gctaatcatt atcatttctt tttccgtaaa atttcttttt
    28801 ccattttctt ttagattata tgttgatttt gctattccta tcttatatgc tagttcttct
    28861 tgggtatatc ctgcttctat tcgaccagct tttaaacttc ttttcttcat gttcttcacc
    28921 ttccaatgtt ttatttttgt gaacttcata cttttattat attcacacaa acaaaacttg
    28981 tcaatacata agtttatttt tttgttaact tttttatttt tttctaaaca taagtttacg
    29041 tttctgtaaa cttatagtat catttaatta ttgggaggtg ttgtttgtgg caacttttgg
    29101 agaacgtttc aaacaattac gagaagagaa aaaacttact caagatgaac tagtttcaaa
    29161 attcaataaa gtttacttta caagttttaa taaatcaaca atatcgcaat atgaaaataa
    29221 taaaagaaaa ccagaaataa atattctaga aaattgggca gatttttttg atgtatcaat
    29281 tgattatctt ttgggtagaa ctcttgttag aaatcacatc gatactgtag ccgcacacag
    29341 agttaatcct cacaaagatt taccagagga agctcaagaa caactcaacg attatattga
    29401 atttttaatg aataaatata aaaaataagg attgaatcta tgttaatatt aaaaaaattg
    29461 agaactgaaa aaggaatttc tttagataaa ttaagcacag acttaaatat aaataaatcc
    29521 actttatcca gaattgaaaa tgggttaaga gaaccaaaag aatcatttat taaagattgc
    29581 tcagactatt ttggagtatc tacagattac ttaattggaa aaattaacat agatgattca
    29641 aataaattaa caaaatgctt aaattcaact tttccaataa ggttaaaaga attaagaaag
    29701 aaaaaagaat taactcaagc agaattatca aaattactta attgttcact aagcaaaatt
    29761 gctatgttag aaactagcaa aagagaacct gttaaagaag acttgttaag aatttcagaa
    29821 ttttttaatg tgtcagttga ttatctactt ggtaaaacct caattgaaaa ttacataact
    29881 actgatgaaa tatccaaaat aattaaatct tatgaaagtt tacctaaaga agcgcaagaa
    29941 catattaata gctacataga gtttttagtt gatagatata aaaaatgaat atttagagca
    30001 gttcactcct gctctttata tataaaaaag taacacatac attcttttta tagggggatt
    30061 tcaatgaaca aactagacgc acttttagac ttagcaaata atgaagagat agaaatttac
    30121 tacactgaca aaatagcaga tgacataaaa ggattgtata taaacagaca aggactaaaa
    30181 attatatcat tacttaattc attaaaacaa aacaatgcta aactaataga aatcttagca
    30241 gaagaattag gacatcattt taccagtgtt ggaaactatg tatcttcaaa aaacagttac
    30301 aaaaataaaa tcttgataga caaaactgaa aacaaagcac taaaatgggc atgtgaattt
    30361 cttataacag aagaagaaat aatacatgtt attaattcac acgctacaag tgtatacgaa
    30421 atagctgaag aattacaagt tagcatcaac ttcttactaa aaagattaga atttctatca
    30481 aaaaagaaaa gcatgttgga cttaggaaat aatagatttt tagtattaac taatttgcca
    30541 aatttctaca tatatgagga tattttttaa actcatttat tctactttta tagatttttt
    30601 acttaataaa tatatatttc aatattatta taataaacta cacataaaag ctaaaaaatt
    30661 gtaagaatat taagaaaata attaagtaaa aaccagataa acaaaattaa gataatattg
    30721 tacataacaa aagtatataa agagcagtta atctgctctt ttatataaac accaaacata
    30781 catacattct aaaagggaga gatactatta tgaaaggtgg agtaagaaaa agaagcaaca
    30841 aatggtatta ctactttgac ctaggcatag tatctgaata aagcaatatc agcttacctt
    30901 ttggtacaaa aaatatttct tacttaacaa cactcaaaaa aattaccaaa taaatataga
    30961 aaactaaaat taagtattta taaagtaaaa ccattataca cagtagtaat tttgatgagt
    31021 aaacacatat tccatatgta ataaagcttg aagttaaaag tggaataaat aaaatctaga
    31081 aaaaattttt attttaagtt aagcttatta tggataataa aattcttgca aatttagaat
    31141 caaatgatac aataagaata taaaatactt gtttgagagg attattttcg tgattaaaaa
    31201 ttttgatgaa tggaaagagt atgatggttt ttcttatggt agtggaagaa gcgagaaaat
    31261 ctggctaatt aatgaaaaaa ctaaagatat aggcttgttt aaattcccta aaacaaaaga
    31321 aactggagaa tattgggcag aaaaattagc ctgtgaaata gctagtttgt taaagattcc
    31381 ttgtgctaat gttgagatag gtacatataa taaaagaatt ggttcaatga gttatttaat
    31441 caataaaaat gatgagaaat tgattgaagg cgtgtggtta ataaacaaat attatccttg
    31501 ttataataca gataagttat atgattataa aaataatgaa tactattcat tgaaaatgat
    31561 ttttaaatct atagaagaat ataacataaa aaatgatttt ctaaaaatgt taatttttga
    31621 ttacttgatt ggaaatagag ataggcatca aaataattgg gctctactag aaagtcaaaa
    31681 taaaattact cttagtcctc tatatgataa cggatcatca ttatgttgtt atgttaatat
    31741 tgagaacata gattctttct tcactgatca tcaaagattt gaagctctta taaatacacg
    31801 ttcaagatca agaataagga tagatggaaa aaataaaaaa gaaccaacac acttagaagt
    31861 gctaaaatat ttaaattcaa attattatga acaaactata gattttgtag aaaagataat
    31921 aaatcttatg aatgaagtaa acataaatac attaatagat aactattcag aaaatattat
    31981 aagcccaaag ttaaaaaaat tgctaaaaat atatctaaaa gaaaaagtgt taagaataaa
    32041 aaatatatat aatttgtgaa agagggataa aatgtcaatt aaaaatggaa aagatttttt
    32101 atatgttatt tggaaagatc caaaaagtag aagacaattt ataattggaa agttagctaa
    32161 aaatggattg tatgaatttt cttatgaata tgaaattgat gaagctatta aagcaggttt
    32221 taatgcttta atatcatttg atgatattag taaaacatat tatagtgaag atttatttag
    32281 cccatttgca agtagattac ctgataaaaa aagaaaggac atcaataaaa ttttggataa
    32341 gtatgatatg tctgagtatg atacatatga acttttaaaa agaagtggtg caagattacc
    32401 tatagataca ctagaatttg tagatccaat tcctttggac attgataaaa atataattag
    32461 atatttttat attgcaggag taagacatta tgctgggtgt gaaggacatg aatgtgataa
    32521 ttcaatagaa ttagaatcaa atgaagctct agttctagag ttagaaccag aaaataaatt
    32581 tgatcctcat gcaatcaagg tatttgataa aaaagagaat catataggtt acattcctag
    32641 atattatagt ggagaattaa caagtataat aaatagagga ttaaagtata attgcattat
    32701 taaatttgtt aacaaagaaa ataattgtga tgaatgtata aaagttaaat tagaaatatt
    32761 atttgacgaa tataattaat gtcttcttgt aaatatagga gacatttttt atataaacac
    32821 caaacaaaca tacattctaa aagggaggga tactattatg aaaggtggag taagaaaaag
    32881 aagcaacaaa tggtattact actttgacct aggcatagta gaaggaaaaa gaaaaaaagt
    32941 agaaagagtt ggtggcaata caaaaaaaga agcagaaaaa gcactaagag aagcactaaa
    33001 tgaatatgaa aactctggca tagtctttga agaaagtaac atcagcttat cagactacct
    33061 agacttttgg tacaaagaat atgttttact caactgtaaa tacaacactc aagaaagcta
    33121 ccgaataaac atagaaaaac atataaagcc aaaactagga gcttacaaag taaaagcttt
    33181 aactcctgca atactacaaa acttcataaa taaaaagtac aaagaggatt attctcaaaa
    33241 tacattacaa gtattaaaag ccatattaca taggtcatta aaatcagcag tccatcctta
    33301 caaacacata cgagaaaacc ctatgcaata tgtaagcata ccaaaaacta aatctaaaac
    33361 agaaactaat aaagttaaaa ctattacatt agaagaattt aatcaaatac taaatatatt
    33421 tcctcaagat tcatttcaac gtatagtttt actaattgga tttcatactg gtatgcgaag
    33481 aggtgaaatt attgcactaa aatgggataa tatagacctt gataataaaa ctatcacagt
    33541 aaagcatact ttgattaaaa aaccaaatgg aatgtttgaa ttagggcaac caaaaacaga
    33601 aagctcttgc agaactatat ttacaggtga cactttaata aaggcattaa aagaacataa
    33661 attatatcaa aagaaaatga aattaaaata cggagaattt tactttgata gtgattgggt
    33721 atgtaccaaa gaaaatggtc aacaagtgaa tactcacact ttagacacta tagtaagaca
    33781 aattcgagta gctttaaaca atgacttcca ttttcattct ttaagacatg cacatgctac
    33841 tctattatta gaaaatggtg ctaacattaa agacatacaa aaccgtttgg gtcatagcca
    33901 attatcaact acaatggata cctattcaca tgtaactgat aaaatgaaaa atgaaactgt
    33961 agatatattt gaaaaaatta caaattagag tttgccaccc aaaaatataa tacggtggca
    34021 aatgggtggc aaaatctaat ttatctattt taaaagctaa aattatcaaa tttatatagt
    34081 cagctatacg cttgtaattt caaggcttta gagtatataa caaccataac taatataagg
    34141 tattaataat aaatctaacg aataaaactt aatatttact taagttgtaa tactattcta
    34201 aattgtataa ttaaaattta ataagttctt aattatatat tcattgattt atttgcaaaa
    34261 ataaaaaatg ccaatctatc tctaaacaaa aactgaatac caataaaaat ttaataaaaa
    34321 attttatcaa tattcagtat aagttttagt tacatatcaa attttccaat gcatcattag
    34381 gtgctacaat aggataaaca tcataatcct tatctatatc acattgtaaa aatacaggtt
    34441 tattaaaatc tatcacatct agaacctttc ctaattcttc catattggat accttatatc
    34501 catcaatatt atatgcattt acgagtttaa cataatccac attttcatta atatcagttt
    34561 gagagtatct ttgattggaa aataacttct gccattgtct taccattcct aatgttctat
    34621 tattgagtag taaaataagc ataggtacat tataatttgc aactgtagct agttcattac
    34681 agttcattct aaaacttcca tcaccagtta ctaaaaccac atttttatct acattaccag
    34741 ctttagttcc aatagctgca cctaaaccaa atcccatagt tcctaaccct gctgatgtta
    34801 taaaactttt attaccttta aaattccagt atttagcaga ccacatttga tgttgaccga
    34861 catcagttac taccacagtg ggtttcttta atgtttcata tttttcattt atttttttaa
    34921 gtatattttg tggatgaaat tcatatgttt gtacaccctc actctttcta aatgtcttta
    34981 tctcttcttt ccaattgcta ttattcttac tttcaaccct ttcaattaat aaactaagga
    35041 ctagctttac atctccaacc aaagatacat tagattctat atttttactt atttcagatg
    35101 ggtctatgtc tatgtgtatt atttttgcat ttttagcaaa ttcagaactt ttacttatca
    35161 ctctatcact aaatctcgca cctatcgcta ttactaaatc tgaattagaa agtgctaaat
    35221 tactttctct acttccatgc attccaacca ttccaagaga tagttcattc tttctatcta
    35281 tatttccaag ccccataagt gtatttagta cgggtgtatc tatcttagtt gcaaattttt
    35341 caagaatttc ctcactgtct gaagatttaa cacctccacc tgcatatatt acaggctttt
    35401 tagattcttt tatgatatct attgcttcat ttaaaagctt aatattagtt tcatcatcta
    35461 gatcaaaatc actcttataa tccatataat catctatttg acataaatca tagtcctcac
    35521 cactaaaatc catttctgct aaaaataaat cttttggaac atctacaagt acaggtcctt
    35581 ttctacctga atttgcaact ctaaaagctt cttttattgt aggaactaat tctttagtgt
    35641 ttctaactaa ataattatgt tttgtcatag ataatgttgc tccagttatg tcaatttctt
    35701 gaaaagaatc ttttcctaat aaactagttg gcacctgccc tgaaattaca accataggtg
    35761 aagaatccat aaaagctgtt gctattccag ttatggcatt tgttgctcct ggaccagatg
    35821 ttgtaaaaca aactcctact gtattagtac ttcttgcgta cccatctgca gcatgaacta
    35881 aaccttgttc atgggatgtc cttatatgtt taaaatcatc tgaataatcg tatagagcat
    35941 cataaagagg tatcacggct ccaccaggat atccaaatat tgtgtcaatt ccctctttct
    36001 ttaaacattc taaaattacc tttgctccat tcatacgcac tactatacac cactctttcc
    36061 ctcaaactaa tccatttaat atatatttat tttaagaatg acatcatact tcttagattt
    36121 ctaccaactt cggcaatttc tgtattatat tcttcttctc tagtttttag gaagttttca
    36181 cgtccttctt cattttcttt aatccacgct tttgcaaatt ttccattttg tatatcctct
    36241 aaaacatttt gcataccttg ttttgcagca tcagttatta cccttttacc agatacataa
    36301 tctccatatt ctgctgtgtc acttatacta tatctcattc tttcaaatcc accttcatat
    36361 ataaggtcga ctattagttt catttcatgt aaacattcaa agtatgctac ttctttttga
    36421 taacctgcat ctacaagcgt tttatatccc aattttataa gttcacttat tccaccacaa
    36481 agtactgatt gttctccaaa taaatctgtt tctgtttctt ctttaaaagt agtttctaaa
    36541 acacctgctc ttgttgctcc tatccctttt gcatatgcta aaactgtctc tgtagctttt
    36601 ttagtgtagt cttgatatac tgcaaatagt gcaggaactc cacttccctt tgtaaataca
    36661 tttctaacta agtgaccagg tcctttagga gctaccatta caacatcaac aaattctggt
    36721 ggttgtactt gattataatg tatattgaat ccatgagcaa aagctaatgt ctgaccttct
    36781 tttaagttat cctttacact ctcttcataa actttctttt gtttttcatc aggcattaac
    36841 atcattgtca aatcactttc ttttgtagct tctgctacac tcttaacttc aaatccatct
    36901 tcttttgctt tttgtgctga tttacttccg tcataaagac ctatcactac atgtacaccg
    36961 ttatctctaa gattttgtgc atgtgcatgt ccttgacttc cataacctaa aactgcaacc
    37021 tttttatttt ttaaaacctc taaatccaca tctttttcat aatacattct tgccattttc
    37081 atttcctccc aatacttttt cttattttta tgatatttat ttttattaga aattcatact
    37141 cattatcatt gttttaatat tattcaacag atttaaattc taaatttata taatcttaca
    37201 attataattt tagaattttc agtttttatt agctacaaca aactaataaa tttattaact
    37261 gtgataaact gataaattca atgacactta tattttaatc taagattttt cttatttaat
    37321 atttatcttt agctttataa aatatattta attaatgtct cattctttca acagttatct
    37381 ctcttatatc atgtagtttt ttcatatggt ttaagacaga atctaaagat gtcttttctt
    37441 catttactat taattttata cctgaattat tcatattatc tacagtcata cttattgatt
    37501 gtatatcaac ctctcttctt cttagagtca tagccactct taaaaaagaa tctattcctt
    37561 ggtttaattt agcatataca gtattttcca taaaaatccc tccttatttt tattattttt
    37621 ttattaattt tatatggatt aaatttattt atatcgctta aaataaaaaa actttcatct
    37681 ttataagcaa tataaatatt acttataagg acgaaagtta tattccgcgg taccacctta
    37741 tttgtagata taaaaaatac atctacccct cttttttcag gtcgggtaat taattacctt
    37801 aaccttagtc atgtaacgtt gacatacgtt tgtacttact aaaaatattc agtacaaaag
    37861 ctccagagtg atcattatac tactgttaac accaattctc agcaacattg gttctctgtg
    37921 gataacacta ttagtattgt tgtctctgtc attgctttta attttttaat tttatatttt
    37981 aattctgttt tgttgttaat atagattata ttccctatca tttacaatgt caatgacttt
    38041 ttttaaaatt ttcagattat ttattttttt cacaataata cttcttttat actttacgcc
    38101 tgtattttgg aataatcgtt ttcagattat tattttatat ccatttattt ttagccaatt
    38161 ttctttatct ttataatcgg gcataatgga ttttactaaa tcatagaatt ttttagagtg
    38221 attcatatgt actaaatggc taaactcatg tattattaca taatcaatta catctaatct
    38281 tgccatagat atttttgagt ttatataaat atttttttga gctgtacaac ttccccatct
    38341 cttattttgt tcttttacct taacacttgc tggaactaat tgcttcatta tttcacaatt
    38401 taatttaaga aaatctattc tctctaatac tgttctttca ctttccatct tataccaaga
    38461 ttttaaagat tttttaataa aatctgtact tgtatcattt gtctgaacaa ctatttgata
    38521 ttcgttaata gttattctac agttactttt tttgacagaa tctttcaaca gttcttttat
    38581 aactagatgg tactcttcac ctaaataaag aaatttttcg ccagttataa acattctttg
    38641 tttgtatact tcttctcttc ctttatattt ttctgacttt ttaagtatcc agtctccttt
    38701 ttcaataatt atatctttta ttattttttt ggatatttta ggaggactca ttacaattat
    38761 tttgccaaat ggatctattt ttatagaaat attttttcta tttttatata ctaaaactgc
    38821 ttttatttca atccctctaa aattaacatt taaagtatca atatctttac tccctgtgtt
    38881 cattagttta tttttcctta ttagctctac aatttataag ttcagcagct atacatacag
    38941 ctatttcagc tggtgtttga gcattcaatt ttatgcctat tggtgcatgt acttttgata
    39001 attcttcctt cttaaaacct tttttcatga gtttttcgta cgtcaatcca atttttgttc
    39061 tacttccaat catacctata taaccagttt ctcttttaag agcccattct aaagcacttg
    39121 aatcatatag atgacctctt gttaaaataa ttatatagct attttcgtca gttggaaaat
    39181 ctggtatatt ttcaaaagaa tctaaaacta ctacttctga ttctgggaac ctttcataat
    39241 tagcaaattc ttctctatca tctattacta ctgttctaaa ttctaaaaga gatagtatca
    39301 cagcaacatc tcttgaaata tgacctgcac caaatatata tgctttactt gtattttcct
    39361 ttagtctctt attgaatttt tctatattac tttttaattt actatctata aaatctattt
    39421 gtatttctcc agtacctcca catatcattc ctagttttgc cacatcagat ttgtttagag
    39481 taaatttata caacattgat tctctatttt ttataacatt aattgcatgt tttatacatt
    39541 ctgcttctac actacctcct cctactgaac caaatatagt agaatcattt ttcacaatca
    39601 tcattgctcc agtttcacgt ggtgttgaac ctgaatgaga cgttattgta gcaaatgcga
    39661 aatcttcatt attttctatt aatcttattg cttgttcata tatattcata taattctcct
    39721 taccagtcta tctaaaaatc ttctatatat ctatcttagt ctttttatta ctttatttgg
    39781 tatcacaaat tctactgctc ccatataacc aggtactact tcatattctt caaaactaat
    39841 cacaagattt ccatttttat taaaataaaa atcttgatac ttatttatag attcaaaatc
    39901 tttcactggg aagtctttat cttggtctat aaaataagat tttgtatcat ctttcttaat
    39961 ttgatttctc atctgctctt ttatattatc acttatagct ctaatatatc tactatcttt
    40021 aaaaaatatt ggtaaattta atagtattcc tctattttta tcaacagtgt aatgcttctt
    40081 agttatagac gaggaatctt ctatctcttc ttttgtcatt tctattgata aaaagtcctt
    40141 agaattattt ttgacattat ggattacatt taggtatcta tgacccttat tattttcctt
    40201 tttcatttta ttttgaaatt cactgtaaat ttttttccca tcttgcaaaa actcattgtt
    40261 taatttattc tctaaagttt tatcttttaa accttttatt ttaggaatac tcacattcac
    40321 ttcataattg ccttgtttaa agttgagact cttagcattt aacaccttta tcgtatcact
    40381 atttgaagtc tcattagagg aactacctgc aaatataggg tttatatcta ctaatgagac
    40441 taataagcat attgaaaata tagaaatttt aaatatagat tttttaagct ttttatatgt
    40501 agtcatattg tccttcttaa aatatgtata tataaactac tattcaattt acaaatcttt
    40561 tattacttta tatggtatta caaactcaac tgcacccata taaccaggtg ctacttcata
    40621 ttcatcaaaa caaattacaa ggtcttcatt tttattaaaa taaaaatcct gatacttatt
    40681 aattgtttta aaatcttcaa caggcaagtc ttttttttga tctataaaat aggattttgt
    40741 actatccttt ttcatttgtt ctctcatttg ttcttttata ttatcactta taacttttat
    40801 atacttatca tctttaaaaa gcattggcaa agtcaaaact atttgtcttt ttttatcaat
    40861 agtataatgt gccttactca catatgatga agcctctatc tcattttttg tcatctctat
    40921 tgatagaaat tttttagaat tattttttac actataactc aaacttaaag atttatatcc
    40981 tttattcttt gatgattgta tcttctccat tctctcttgg aattgatgat aaagcctttt
    41041 tccatcttcc ataaaactac tgtttaatct ttgttctaag tttttatctt ttaaacctcg
    41101 tattttagga atatcaatct cagcttcata attttcactt tttaaactat aattttttat
    41161 agttattact tctactatta ctccaattac aggtatctta gaaaaattgt ctgcaaatac
    41221 aggacttata tttactaata ctgtaaatat acatgcaaag gctattgcaa ctttaaatct
    41281 taatctattt acttttttat ttttctctcg atatatgttt tctatttttt catgatttat
    41341 tatagtatct aatttcttgg gtattggaat tttatgatac tcatctttta acttatttaa
    41401 cctttctttg cttgtcattc tttttatccc tactcttcaa tttttaattt tagttttttt
    41461 aaagcagtat ataacctagt tttaacagta ttcacatttt catccaatat tattgctact
    41521 tcttcaatct ttaaatcttc aaaaaatctt aatattataa tagatttgta ttcaataggt
    41581 aattcttcca aagctcttct taaatccaaa tcagtatatt tatcatactc tccagtctca
    41641 tctataaggt caatatctgt catattatta tatttacggt tttttctaat aaaatctata
    41701 ctcgttctaa ctaaaatttt ataaaaccat gtctttatat aatttacttc tttaatattt
    41761 tccactgagt atagtgcctt atacatggct tcttgaacca catctaaggc atcttcttca
    41821 ttcttcgtat aactatatgc tattctataa aaagagtctt tattatcgat aatgtatttc
    41881 tcaactttcg ttctctttaa actcttaaac attcctgtaa aatctccctc cgattacagg
    41941 ctaggattga gctgttagaa attctatctg tctattttag catcttcatt cagttttttt
    42001 atatgctcaa tatacttttc atttaaaata gattctctta ttaaactctg acactcatta
    42061 aaactttttt gtctttctct tttatcagta actttgacta tatgatatcc ataactagtt
    42121 tcaaacacat ttgaaacttc atttttcttt aatttgaaca cttcctttgt aaactcagca
    42181 tttttatcat cctttgtaaa atatcctagc tgtcctccat tttttccagt tgctttatca
    42241 tccgaatatt ttttagctaa actttcaaaa ctttctccat ttttgatttt agtcaatata
    42301 ttatctgcct tcttcttcaa agcttctttt ttatctttgc ttacttcttt tttattttta
    42361 tctaaagtcg atataagtat ctgagaagca cttacttctt ctacattaaa atctttttta
    42421 tgacttgtat agtaggcttc catatcatta tcacttatgt ttattctatc ttcaaaggcc
    42481 tttttatttt tatctattgc taaatctcta gatatttctt gttttaaata ttctttatca
    42541 acacctgctt tgtccatttt ttccttatag cttgtattca aattcatctt atcttctagt
    42601 tcttgatatt ttgagtctac ttcactttta cttggagtta gtcctttatc cttagcctct
    42661 tgatataaaa gttcattatc caccataaac gatattataa cattttctaa tttatttcta
    42721 ccttttttat ccaaagtttt ttctaattca tctaaagact gcccatttat ataattatta
    42781 gtagcagata aaactgcttt tgtttttttg tactgttctt ttgtgatttc cacatcattt
    42841 acttttgcta aacttttatt acaaccaacc atcatcaaag acaatattcc gaccattcct
    42901 atataaagtt ttttattcaa tatacctaca cctcactcat ttttattctc aatttctaaa
    42961 aatatcaatc aatattctta tattttaact actctaaatt acgtaaccaa taattttttg
    43021 gtatttggtt ttgatctact ggcgcaaatt cataaccttc atctgataag aattttaaaa
    43081 ctgaatctaa agcttctgca ctctgttttt tctcatgcat taaaactaca agttggtctt
    43141 tatcttcacc ttttctattc ttaatatgat tttttacatt ttgtacaatt tgagaagaat
    43201 tagatctcca atcttctgta tctaagtccc agtcccaaat tttatatcca gcatcaacta
    43261 aagcctgata agaggcttgt ggtgtatatg gtttgcttcc ataaggtatt cttattactt
    43321 ttgaatgttt accagttatc ttataaaaag tttgatcatt tgtatcaaat tcctcttttg
    43381 ctgatgtact agtaacataa agcttgtgaa tatcatgtga tacactatga aaacctgctg
    43441 tgttcccatt tttaattata tttttaactt gctgtggata ttccttcata ttcccatcta
    43501 tcataaaaaa agttgcttta acattgtatt tatttaaagt ttttatcatc tcgtcagtaa
    43561 atttagatgg gccatcatct atagttatgt atgctatttt tccagaactt ttacttggcc
    43621 ctttactaac ttctattgat gtattagata ctatatctgt aatagtagaa cttgacagtt
    43681 ttgcatttct actaatttca ttttccttca tatttaaaga gctgacgcga acaccaacta
    43741 ctataaaagc cagacacact acaatagcac ttaaaagcaa atacaacttc tttctattta
    43801 tttttttctt tttatttctc atcgttcgac ctccaatatt actatatttt tacatttata
    43861 tttttgtagt attttatccc aaaaatttac actacatata tatagacgtt gaacttcgac
    43921 aaaaagtttt gcttaatttt taaaatttgt tatttcggtg attattatgc tgtataattt
    43981 ctgtata
//
LOCUS       DACTGW010000006        42642 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.006, whole genome shotgun sequence.
ACCESSION   DACTGW010000006 DACTGW010000000
VERSION     DACTGW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.006"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            121..1338
                     /locus_tag="I9573_000271"
     CDS             121..1338
                     /locus_tag="I9573_000271"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HAT4794916.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1433..1813
                     /locus_tag="I9573_000272"
     CDS             1433..1813
                     /locus_tag="I9573_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAT4794917.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2097..2579
                     /locus_tag="I9573_000273"
     CDS             2097..2579
                     /locus_tag="I9573_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAT4794918.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2600..3415
                     /locus_tag="I9573_000274"
     CDS             2600..3415
                     /locus_tag="I9573_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAT4794919.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3466..5016
                     /locus_tag="I9573_000275"
     CDS             3466..5016
                     /locus_tag="I9573_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAT4794920.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5113..6465
                     /locus_tag="I9573_000276"
     CDS             5113..6465
                     /locus_tag="I9573_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HAT4794921.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAESLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKVFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6596..7354
                     /locus_tag="I9573_000277"
     CDS             6596..7354
                     /locus_tag="I9573_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4794922.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIYNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7401..8120
                     /locus_tag="I9573_000278"
     CDS             7401..8120
                     /locus_tag="I9573_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAT4794923.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8244..9644)
                     /locus_tag="I9573_000279"
     CDS             complement(8244..9644)
                     /locus_tag="I9573_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAT4794924.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9648..10538)
                     /locus_tag="I9573_000280"
     CDS             complement(9648..10538)
                     /locus_tag="I9573_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT4794925.1"
                     /translation="MTEKQRLIINIAQKIFDQKGFQNTSISDVVKECKMSKATFYKHF
                     ETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRTTINTYI
                     RENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDALIHQFI
                     YIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYFSLHNKS
                     LFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAYLEKEDT
                     LRPKVLLLNSIKNQLKKETL"
     gene            10817..11464
                     /locus_tag="I9573_000281"
     CDS             10817..11464
                     /locus_tag="I9573_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAT4794926.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11789..14428
                     /locus_tag="I9573_000282"
     CDS             11789..14428
                     /locus_tag="I9573_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAT4794927.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14486..15589)
                     /locus_tag="I9573_000283"
     CDS             complement(14486..15589)
                     /locus_tag="I9573_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HAT4794928.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSSK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            15950..16771
                     /locus_tag="I9573_000284"
     CDS             15950..16771
                     /locus_tag="I9573_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAT4794929.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     gene            17148..18464
                     /gene="argH"
                     /locus_tag="I9573_000285"
     CDS             17148..18464
                     /gene="argH"
                     /locus_tag="I9573_000285"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAT4794930.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18713..19051
                     /locus_tag="I9573_000286"
     CDS             18713..19051
                     /locus_tag="I9573_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HAT4794931.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19178..20467)
                     /locus_tag="I9573_000287"
     CDS             complement(19178..20467)
                     /locus_tag="I9573_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAT4794932.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20547..21251
                     /locus_tag="I9573_000288"
     CDS             20547..21251
                     /locus_tag="I9573_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAT4794933.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21343..22389
                     /gene="selD"
                     /locus_tag="I9573_000289"
     CDS             21343..22389
                     /gene="selD"
                     /locus_tag="I9573_000289"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21391..21393,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAT4794934.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDNPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22407..23819
                     /locus_tag="I9573_000290"
     CDS             22407..23819
                     /locus_tag="I9573_000290"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAT4794935.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVAELRKAFN"
     gene            23933..25816
                     /gene="selB"
                     /locus_tag="I9573_000291"
     CDS             23933..25816
                     /gene="selB"
                     /locus_tag="I9573_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAT4794936.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALEALISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            25921..28659
                     /locus_tag="I9573_000292"
     CDS             25921..28659
                     /locus_tag="I9573_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HAT4794937.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSIILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(28778..29722)
                     /gene="manA"
                     /locus_tag="I9573_000293"
     CDS             complement(28778..29722)
                     /gene="manA"
                     /locus_tag="I9573_000293"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAT4794938.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            29895..31256
                     /locus_tag="I9573_000294"
     CDS             29895..31256
                     /locus_tag="I9573_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAT4794939.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSIVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31344..33233
                     /locus_tag="I9573_000295"
     CDS             31344..33233
                     /locus_tag="I9573_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT4794940.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            33248..33703
                     /locus_tag="I9573_000296"
     CDS             33248..33703
                     /locus_tag="I9573_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT4794941.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            33762..34079
                     /locus_tag="I9573_000297"
     CDS             33762..34079
                     /locus_tag="I9573_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAT4794942.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            34104..35366
                     /locus_tag="I9573_000298"
     CDS             34104..35366
                     /locus_tag="I9573_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAT4794943.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFIDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            35471..36535
                     /locus_tag="I9573_000299"
     CDS             35471..36535
                     /locus_tag="I9573_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAT4794944.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKIGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKIIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            36589..37767
                     /locus_tag="I9573_000300"
     CDS             36589..37767
                     /locus_tag="I9573_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative C-S lyase"
                     /protein_id="HAT4794945.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKNIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(37958..39016)
                     /locus_tag="I9573_000301"
     CDS             complement(37958..39016)
                     /locus_tag="I9573_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAT4794946.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTIFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINEIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            39083..39454
                     /locus_tag="I9573_000302"
     CDS             39083..39454
                     /locus_tag="I9573_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAT4794947.1"
                     /translation="MKKKDILLILGILVVIIACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            39491..40165
                     /locus_tag="I9573_000303"
     CDS             39491..40165
                     /locus_tag="I9573_000303"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005978738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAT4794948.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            40414..41253
                     /locus_tag="I9573_000304"
     CDS             40414..41253
                     /locus_tag="I9573_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF348 domain-containing protein"
                     /protein_id="HAT4794949.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            41496..41592
                     /locus_tag="I9573_000305"
     tRNA            41496..41592
                     /locus_tag="I9573_000305"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41530..41532,aa:Sec,seq:tca)
     gene            41743..42450
                     /locus_tag="I9573_000306"
     CDS             41743..42450
                     /locus_tag="I9573_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HAT4794950.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
BASE COUNT        16712 a         4700 c         7416 g        13814 t
ORIGIN      
        1 gttttttgtg attaattaaa aaattataaa aatcgtttac atgttgattt ttaaaaaaaa
       61 aagatataga attatataga aagtaataaa caaaaattgg atttacggga gtggaatgaa
      121 atgatgaatg ttaccttaac agatgtgaaa gaggctagag agaccattaa gaacatagta
      181 aaaagaactg acttacttga aagtgttaaa cttagcgaaa aaacaggagc aaatgtgttc
      241 tataaatgtg agaatcttca aaaaacaggt tcttttaaat taagaggagc ttgtaacaaa
      301 atagcgagtt taacagatga agaaaaggca aatggagtta tagcttcaag tgcagggaat
      361 catgctcaag gagttgcatt gggagcaaaa atgactggta taaaatctac aatagtaatg
      421 ccagcaactg cgcctttagc aaaagttagt gcaactaaag gttatggagc agaagtagtt
      481 ttaaatggtg cggtttatga tgatgcatat gcaaaagctg ttgaaataca aaaagaaaca
      541 ggagcaactt tcttacatcc attcaatgat aagtatgtta tagcaggaca aggaactata
      601 tctcttgaaa ttttcgaaca attagacaat aaagttgata ctatactatg cccagttggt
      661 ggaggaggaa tcatctctgg tgttgctgtt gcagcaaaag cattaaatcc taatgtaaaa
      721 atagtcggtg ttcaaactgc aaatatacct tcaatgaaag agtcaataaa aaatggaaaa
      781 gtaacaacag catttaatga tactacaata gctgatggta tagctgttaa aactccagga
      841 gatttaactt ttgaaataat aaatgaattg gttgatgaaa tagtagtggt tgaagagaca
      901 gaaattgcag aatcaatatt atttatgatg gaaagtcaaa aaatagtttc agaaggtgct
      961 ggtgctgtat gtacagctgc tatattaagt ggtaagtatg taccagcaaa agatgaaaat
     1021 gtagtttgta taatatctgg tggtaatata gacataaata cgttatatag aataataggt
     1081 gttgctttag ctaaagaagg aagaagatat tcatttagta caattatgga agataagcca
     1141 ggaaactttg ctgagttaac tagaataata agtgagaatg gtggaaacat tttaagtgct
     1201 aatcaaggta aattatcagc aggggaagct ctaggaaaac aaagtgcgga atttatatta
     1261 gagacaatag attatgacca tatagctaga ataaagaaag ctatagaaga aaaaggtttt
     1321 aaaattatag aattataata aaaagtgtat ataatataca agaaaaatag tataatagtg
     1381 tatgaagata cattttatgt gtattagatt aacgaattag gaggatttta aaatgaaaca
     1441 tgaagtaata catactaatg atgcaccagc agctttagga ccatattctc aagctataaa
     1501 agctggaaac ttattatttg tatctggaca agttcctctt gtaccagaga ctatggaagt
     1561 agttgaagga gatgtacaag ctcaaactgc tcaatcttta aaaaatctta aagctatatt
     1621 agctgaaagt ggagctgatt tttcaaatgt agttaagact acagtattta taaaagatat
     1681 gaatgaattt ggggctataa acgaagttta tgctgaatat tttggagaaa ataaaccagc
     1741 aagagcttgt gtagaagttg ctagattacc aaaagacgtt aaagttgaaa tagaagtaat
     1801 agctgtatta taaaaataca atatttttaa tttgaaaagg ctgaaaaact atattctagt
     1861 gattcagtct ttttataaaa aaataaatca caataaagaa catgataaat attgtcgaat
     1921 taaaaaataa aattaaaata aatattgcaa tatttttgag aaggttttat aatgagtata
     1981 taatttgaaa cgtgtaacta ttaaattagg catgagttct tggatatttt tatcctgagg
     2041 attcatgcct tttttgtatt gaaattcaaa aatctattta ataaggagga agtattgtgt
     2101 taaatttttt caagaaaaat aaaagctata agttacatgc agtagtgagt ggtaacagca
     2161 ttaatattga gaaagtaaac gattctgtgt tttcaaaaaa gttaatggga gatggagttg
     2221 caattattcc taatagtaat gttgttgtag ccccttgtaa tggtaaagta actgtgctta
     2281 cagaatcaaa gcatgccttt ggtatggtct cagatgaggg tgttgaaata ttagtccaca
     2341 taggaattga tacagttaat ttacagggtg aggggtttaa aaatgaagtg tctcaaggag
     2401 atactgttaa aaaaggtagt ccaataataa gttttgaaag agaaaaaata aattctcagg
     2461 gaattgattg tacaacaata ataattgtat taaatcatag cgaattttca gaaataaatt
     2521 gcatggtaga aaatgaagtt gttgcagggc aagatactgt tattgaaatt atgaaataat
     2581 aggaagggat taattcaata tgagagtagt aaaaatcttt aataataatg cattatctac
     2641 agtaactaat gataggaaag aagctatttt actaggtctt ggtataggtt ttaataaacg
     2701 tccaggagat aaagttaatg aagataaaat agaaaagatt tattatgttc aagaccacat
     2761 gcaaacaaaa tttttagaat tattaaaaaa tgttagtcca gaagtgatgg atgcttcaca
     2821 acaaattata agtcttatac caggaaatga gggaaaattt aataataaag ggatattatc
     2881 tttagtggaa catattagtt ttgcaataga tagaatgaaa aataatgttt ttttacctaa
     2941 tttaatgctt ttggatataa agatgatgta ttcaaaagag tttgaattag gagtaaaagc
     3001 tttagaaatt atatatagag tatgtcatat ccatttgcct gaagatgaag caggttacat
     3061 agctttacat tttgtcaatt tacaatcaaa tgataatttg gcatatgata ctttaaaatt
     3121 tgtaaaaggt agtattgatt tgattaaaga atgttatgga ttagaattag acgaatcgag
     3181 tttaagtaca ttacgtttta gaactcatct taaatttctt gctcaaagaa tttttaagaa
     3241 tgaaatttgt caggatgata aaatgattga aatgtatgat tatttaatta acaatcatcc
     3301 taaaaataaa gaatatttag aaaagttaaa tgtatatatt gaaaaggagt ttaggtataa
     3361 attagataaa cctgaaaaaa tttatctact actacaccta acaaaaatct tataagaatg
     3421 agatgaatta gtaatagaaa tatataattt taggggggga taagattgaa agaaaaacta
     3481 cttggaggac tggaaacatt tgcaaaggca atggttcagc cattaatgta tttatcgagt
     3541 gcaggaatat taatggtggt aggtgtacta cttacaaatg atatgttatt aggagtactt
     3601 ccatttttta aatgggagcc aattcaaata tttggaagcc taatttataa ctgtataatg
     3661 gctattatta ataatcttgc tgttatattt gctataggta ttcctgctgc actagcaaaa
     3721 aaagagaagc acaaagcagc gttaatagga tttatgagtt atataatata ccttacagct
     3781 tccaacacta tgttaacatc actaggtcaa ctagcaaaag aaagcaagat gcttggactg
     3841 atagggactg gacaagctac tattttaggt attcaagttg tagatacaag tgtatttggt
     3901 ggaattattt tagggtgtat agctggatat gtatttaatc gtacaagcaa caaaacattt
     3961 aaaggagctt tacagatata ctcgggagca aattttagtt ttatatgcat gttctttgta
     4021 tccattttaa ttggagttgt gagtaacttt atatggccat tagctcaaca aggaatagct
     4081 tctcttgcaa atgtcatcaa agatacaggt acatttggtt tattcttata tggtttttta
     4141 gagagaatat tagtaccaac tggacttcat catttaatct atacaccatt tcagttttct
     4201 gatttaggag gagtgcttaa agttggtgca gatacagttt caggagccta tgctatagtt
     4261 atgacagaaa tgaatttacc agttgagcgt ttctcagata gtatatacta catggcaact
     4321 ggatttacaa agatgtttgg ttatataggt ataggagctg cctttattta tactgcatat
     4381 aagcaaaata agaagaaagt taaggcaacg attattccac taataattac ggcagtatta
     4441 gcctctgtaa cagaaccatt agactttatg tttgtattct gtgcaccact tttattcttt
     4501 gtacatgcag ttatagctgg tctttttata gctttattaa aaatatttga tgttactgca
     4561 ttatgtggag ggaacttatt agtatcaatt ataatgaata ctacagtagg tgtagaaaaa
     4621 acacactggc cagtaatgat gatattagga cttgcacaaa ttattgtata ttttgtagta
     4681 tttagttttt taattaagaa atttaattat aaaactccag gtcgtgaaga tgagagtaca
     4741 gctacaggcg aagaacaatc aaaacaactt aatttagatg caggtataga aaatataatt
     4801 gatgggttag gcggaaaaga aaatattaat acagtagaaa attgcatcac tagattacgt
     4861 gttaatgtaa aagatgaaag taagattaat gaagatataa ttaatttaac gccaaatagc
     4921 gggattgtaa ggaaaggaaa agatatacaa ataatttttg gacttcatgt gcatgaggta
     4981 agacgtgcag tagaagattt tttagaagct tattaaagat atatatttat atagcaaggg
     5041 taaataacag ctttaagtgc tgtttactat ttattaaaga tacatataat ttatagagga
     5101 ggaataatta ttatgattat tactattgtt ggtggaggaa gcacatttac tccaggtatt
     5161 gttaagtcaa ttgcgttaag agaaaaagag ttagagcttg aggaaattcg cttatttgac
     5221 attgataaag aacgccaaga taaagtagct gtagttgtca actggatact aaaagaagat
     5281 gttaaaacag atgttaaact aactgtaact aattgtgaaa aagaagcgta tacagatgct
     5341 acatttatat ttgcacaaat gcgtgttggt aaatatgaaa tgcgtgagca ggatgaaaaa
     5401 attccattga aatatggttg tgtaggtcaa gaaacttgtg gttgtggagg gatggcatac
     5461 ggactgcgta caatacaacc aatgataaag gttattgatg atgtagagaa atatgctaaa
     5521 aaagattatt ggatattaaa ttactctaat cctgctgcaa tcgtttctga agcttgtcgt
     5581 aagttaagac caaatgcaag gattataaat atctgtgata tgccaatagc tattatagat
     5641 atgatagcgg agtcattaaa tatcaatgac gttcataata tacgctatga ttattttggt
     5701 ctaaatcatt ttggctggtt tacttcaata gattataaac atagagactt aatggaagag
     5761 attaaggaat acataaaaga aaataaaata ttattaccaa aatcttatat agaaaactta
     5821 aataaaagtc gtagtacagg aaatcgccat gctgtaagta gctggataca tgtttgggaa
     5881 tctgtttata caatgttaga aagcttccca aaatatctgc caaacactta tttaacatac
     5941 tatcttttaa gtaaagaaac agtggaacat tcaaacataa atcgtactcg tgcaaatgag
     6001 gttatggaaa gccgtgaaaa agacttattt gaaggtgtga gacattactt agaaactgga
     6061 gaaatttctg aaaaagtatt ttatgcaggt tcacatggag attggattag tgatttggca
     6121 gtttcaatta aaaatgatac acgtagtcgt tttttagtta taactgaaaa tcgtggagct
     6181 ataccaaaca tgccatatga tgcaatggta gaaattccag catatattgg taaagatggt
     6241 ccagaagtta ttgctcgagg tccaatacct ctattccaac aaggattaat gatgcaacaa
     6301 cttaacagtg aaaaattgat agtagaagct tatatagaaa atagttatga aaaagcttta
     6361 caagcattca cattaaataa aactattcca agtatgaatg ttgctaaaga aattttggat
     6421 gaaatgatag aagttaataa ggaatactgg ccagaattaa gatagtagga taaaagaaag
     6481 ggtttttatt taataattag atatgttttc agagtctttt atcaaaatgt attcacaaac
     6541 atatggcaat atattaaaat aaagagtgat atttttagaa gaatggggtg atgcaatgaa
     6601 cattataaat aaaatttttt ggcaagaaga taggattggc atgattacaa ataatttaga
     6661 ggcagataca catagccact gcatgttaca actgttctta gggatagaag acagcattga
     6721 aataacagtc aatgaaaaat tagttaaatg taattgtatc atagtagata aaaatatctc
     6781 tcattcattt tcagcaagaa aaaaagttta ttactctgct attattgaac ctacatctat
     6841 atatgcagaa cagcttacct caaaaatgaa tgattttggc tactggatat gtgacaatga
     6901 tggattagag aaattacgtc aacaaggaac ttttttgata tataactcta gtaaagaaca
     6961 atatttaaga tttatggaaa tgcttaataa ctatttgaat atacctataa ctctcaaaca
     7021 ttatgatgac agaattactg aattgctcaa tttattacgt acttgtaatt gtgataatca
     7081 tactatttca agttttgcag ataaagtgtc cctatcagca agtagattat cacatctgtt
     7141 taaagaacaa atagggatac ctttaaaaag ctatatctta tttcatcaaa tggagtgtgc
     7201 ttttagagaa ttactttctg ggaaaaatgt taccgaagct tctatgactg ctggattcga
     7261 tactccatca cactttgcag ggacagttaa gcgaatgatg ggaatgcctg tttctttatc
     7321 tttgaaagat agcgaatttt tgaaagtata ttaaatattc tcgttatata atgaagttgt
     7381 actaagaaag gggctttgtt atgaataaag aatatttaga ggaaacaaaa atgttaaatt
     7441 ataatgaacc acaacttaag cttttggtat catcaaagaa ttggttggaa ttagatgatt
     7501 ttcataaaat aaaatctatt tatgagtttg tacagaatga tattttattt ggatataatg
     7561 cttctgatat gttaagcgca acacaggtat taaatgatgg tatgggacaa tgcaatacaa
     7621 aagctactct tttaatggca ttattacgtg cagtgaatat cccttgtagg cttcatgctt
     7681 ttgatgtaac aaaagatttt caacgaggtg ctacatccaa attgatttca ctattagcac
     7741 caaaatatat tcttcatact tgggtagagg tattttatca agataggtgg attgctctgg
     7801 agggtgtcat aactgataaa aaatatttag aagcaattca gaagaagttt tttaatcatg
     7861 gtggaacgtt taaaaagtat gcaatagcta caaatgattt gaaaaataca tctattgatt
     7921 gggatggtaa agatactttt atccaaaaag aagctatcgt gtatgattat ggtatttttc
     7981 catcaccaga tgtatttttt tctactcatt cacaacatat gtcaaaatta aaaaatttca
     8041 tatatgtaca tcttatcaga aaaataatga caaaaaatgt atgtaaggct agaaacaatt
     8101 atattgataa aaacgaatga gtgtttaaga ggtaactata taggaaatat atagaataat
     8161 taaaactgta ctggttaaaa tgaataaaaa ggagttatca tagagcaaac ttaaattgtt
     8221 cataggataa ctccttttct gtattaactt ctaataattt gatgtctttt tataaaacat
     8281 atagatacaa ttaaaccagt aaatgcaaga gtagctgcta ttgaaaatgc aacatctatt
     8341 ccatgaatgt tagacagcat gtttgaactc atatgagctt tcttagtagc acttgtcatt
     8401 atagtaataa gtatagcaga acctatagct ccagatatct gacggagtgt gttattaatt
     8461 gctgtggcat gagatataag ttctctatct aatgttttaa ttccccatgt agttaatggc
     8521 attaaaacca ttgaaatacc aatcatacga atacaataca tcaatgacac atgaattaaa
     8581 gatgtatctc tgcctaaaaa agcgaaggat aaagttccta aaaacaaaca tcctgttcct
     8641 aatattgata gagcatgagg tccatattta tctaaatgac gtcctgcaat tggattgagt
     8701 acaaccatca atatagcacc aggtaacatt aatgaacctg aagataatgc agaaaatcct
     8761 cttacagatt gaacgtacag aggaatcatt aatgtagcag acatcattga ggcatataca
     8821 atcataatta atattgtaga aataagaaat ggtttatttt taaaaaccct caattctaaa
     8881 aaaggttctt tagattttaa ttgacgcaat gtaaataaaa ttaatgacat aattcctatt
     8941 aatattggta aatatgtggc aatattagtc cacccataat taccttgatt agtaaatcca
     9001 attaataaac caccaaaacc tagagatgat agtactattg aaggtatatc taactttagt
     9061 ttttgagttt ccccaacatt tcttaataga ataaatgata gtataacatc taatatagca
     9121 ataggggaaa gaaacagaaa taaactatgc caaccaaatg aatctactaa ccatcctgat
     9181 aaagttggac ctattgctgg agcaaatcct actgtaatac caactaaact catagctgca
     9241 ccacgttttt cctcagggca taaatagagt ataataactt gtaaaagtgg cattagagaa
     9301 ccagaaccca ttgcctgcaa cacacgagca atcaacatag ttgaaaaatt attagaaaaa
     9361 agtgcaataa tacatcctat agaaaagata gacattgatg taataaataa ctttctagta
     9421 ctaaatcgat ttattaaata agctgttgtg ggtacaataa tacctgcaat aagtagataa
     9481 attgttgtta accactgacc aatatcagca gtaattttaa aatctgacat tatattaggt
     9541 aatgctgttg acaatagtgt ttgactgaga gaacttataa aagcaccagt cattacaatt
     9601 gctattatta aatttccttt tttattgtta ctttgggttt ccataattta taacgtctcc
     9661 ttttttaatt gatttttaat gctgttcaat aaaagtactt ttggtcttaa agtgtcttct
     9721 ttttctaaat atgcaatcat tgcatccata attaaagaat catattgttg attttttccc
     9781 tcgacgtata atttttttat tgcttctagt aatttaggat tttcaaggag agaagtatca
     9841 gttttgatta gttcttctat atcttgtatc gttttaaaaa atagcgattt attatgaagg
     9901 gaaaagtaaa cttcatcttc ttgtttatgt aaaaacattg atttttcaat taaaggattt
     9961 agattatcca tattttctac aactaaatct agaatttgta tagtaaattt ggcaataaag
    10021 tatacattta tttctctttt ttgtacatga ataatgtata taaactgatg aattagagca
    10081 tccaataaga aaactaaatc aaatataatt ttttctattt tatcaccata gttatcaaat
    10141 aagcttaatt tgtattcatt caataagtta gctcgtgatt ttgtttgcaa tttacttgta
    10201 gccttacgtt gctcttcaga aaaattttct cttatatagg tatttatggt agttctagaa
    10261 aaaatatttt tccatacagc aattatcttt ctgtttaact tttctgatga tggaatgtca
    10321 ttattttcgt ttatggaatg tataatttct aaaaactttt catcataata attgattatt
    10381 tcgcagataa aactttcctt agtttcaaaa tgcttgtaaa aagtagcttt agacatttta
    10441 cactctttta caacatccga tattgaagta ttttgaaaac ctttttggtc aaaaattttt
    10501 tgtgcaatat ttataatgag cctttgtttt tctgtcactt tattatctcc tttcacgcaa
    10561 aaacgtttag tactatatag tacctacaag ttttaataaa tatattatac atcatttgga
    10621 aaaaaaatca atatatagac tgtaatatga aatttttgaa aaatttgttg ttatttttag
    10681 tcgtataaaa aagaaactta agagtttctt aagaattgtt taaagaagtt gcaataaatt
    10741 agtggtatga ttatagtatg aaagagaatg tcttgaaatt tttctgtatc aaattccaat
    10801 tatggaggtg tagtatatga aaaagaaaat ttgtgtagca ataatttttg ctctatttat
    10861 agtttttgta gaaatgttta ttgatagctc tagcgaagga agtaaaatta tgacagctga
    10921 attaagtaaa tacaggaaag gcgtaaatgt attatcaata aacaatatta tagtagcgaa
    10981 taaaaaatat agtttgccca aagattattc tcctcaggaa agcagtgaag cgagagatgc
    11041 tttttataaa atgaataaag atgctcaaaa gtcaggatta aatttaaaag cttttagtac
    11101 atatagaagt tatgaatatc aagatagatt atttaaatca tatgtaaaag aacatggaga
    11161 aaaagaagca aatagatttt cagctaaacc tggagagagt gagcatcaaa ctggacttgc
    11221 ttttgatatt ggaggagatg accagtcttg ttgggcaaat aaaaagttca acaatacaaa
    11281 agaagctaag tggttgtatg aaaatgcata taaatatggt tttatactta gatatccaga
    11341 aggtaaagag aatataacag gatatatgta tgaatcttgg cactatagat atgttggaac
    11401 tgaacatagc aaaaactttg caatgaataa tttgacttta gaagaatatt tacatataaa
    11461 ttaagagatg gtttgttaaa attaggttaa aataaaaaat aacctagact attagctgtg
    11521 taaagtgata taatgtaaat acaatttaag ataactactc gaaggggagt agcttacacg
    11581 aaagtggtaa ttaatcgtca agacagtaga aatactcggt taattagcaa gggattcctt
    11641 gatagcaaga ccttcaaaat aagcattagg tatgtttgtt ttggggtctt tttttattca
    11701 aggaattttt atttaaaaaa atttaaattt caagtataca taagagcccc caaacataaa
    11761 ataaatttat tgaagaggag ggcaacaaat gtcttacaaa agtacttatc aagaagtaat
    11821 taaaaattta gactcaagtt tatctggttt gagtaaagaa agagcagaag agttgttgga
    11881 aaaaaatgga gctaatgaat taaaagaagc agataaggtt cctacttata agcttttcct
    11941 agaaagtttt aaagacccat tagtaataat tctattgata gctgctttag tacaaatatt
    12001 tttaggtgaa acagtggagt ctataataat ttttgcagtt attattataa actctgttct
    12061 gggtgttgta caaacaaaaa aagcagaaag ttcattagaa agtttgaaaa atttatctgc
    12121 accaaacgca aaggtaataa gagacaataa aaaaatgaca attccagcaa aaaatctagt
    12181 agttggagat attgtattct tagaagctgg cgattacata ccagcagatg gaaggcttat
    12241 agaagcacaa tctctcaagg ttgtagaagg gatgttaact ggagaatcag aaccagtttt
    12301 aaagcataca gaaaaaataa gtgaagatgt aagtttaggt gaccagaaaa atatggtatt
    12361 tagtggtgct gtagttgttt atggtagagg ttcttttgta gtgactggaa caggtatgga
    12421 tactgagatg ggtaaaatag ctgggctttt agaaagtgct gaaaataaac ttacaccatt
    12481 gcaacaaaaa atagaggatt ttagtaaaaa acttggtgta ggtattgtaa tacttgctct
    12541 tttaatattt ataattcaag tagcaagaaa ttactttatg gctggaaatg gagaaatgac
    12601 acaaattatt ttagattcat tcatgttctc tgtagcagta gcagtagcag ctataccaga
    12661 agctctatca tcaatagtca ctatagtatt ggctgttgga acaaatagta tggcaaaaaa
    12721 acaagccata ataagaaaat taccagcagt agaaacttta ggttcaacaa gtgtaatatg
    12781 tactgataaa acaggtactc ttacacaaaa taaaatgaca gtagttgatt attatatgta
    12841 tggaactaat aatgatgagt tagataaaga aaaagtaaac tattcttatc atgctaaatt
    12901 attgactatg gtttctacat tgtgtaatga ctctcatata actagtgatg gtaaagaagt
    12961 tggtgaccca acagaagtgg ctttaataaa ttattcaagt aaaaatgatt tagattatga
    13021 taaattaaga agtaagtata tcagaataaa tgaaatacca tttgattcag acagaaagct
    13081 tatgtctact gtaaatagta tatatggaga ttatataatg tttacaaaag gtgctccaga
    13141 tattgtgttt agtagatgta aatatgcttt gaaaaatggt tcaaaagtag atataacaga
    13201 tgagataata gaagaatata aacataaaaa tgaagagttt tcaaatagag cacttagagt
    13261 attagctttt gcaataaaag atgtcccaga agaagatttt ataccttcaa ttgatgacga
    13321 acatgacatg acattggttg gaataatggc aatgatagac ccaccaagag aagaagtaat
    13381 ggatgcagta aaagaggcaa agagtgctgg tataaaaact gttatgataa ctggtgacca
    13441 taaaacaaca gcagcagcaa ttgcaaaaga gattggtata atggaagaag gagacttggc
    13501 tcttactgga aaagagcttg atgctttgag tgaagaacaa ctatatgaaa aactagaaaa
    13561 tataagtgta tatgccagag tctcgccaga aaataaaatt cgtatagtaa aagcatggca
    13621 acataagaat aatataacag caatgactgg tgatggtgtt aatgatgcac ctgcattaaa
    13681 acaagcagat ataggaattg gtatgggaag tggtactgat gtagcaaaag atgcttctgc
    13741 tatggtattg gttgatgata actttgcaag tatagtaaat gctgttgaag ttggtagaac
    13801 agtatacagt aatataataa aatcaattac ttatctattt gcaggaaact taggagctat
    13861 agtatccata gtatttgcag tgtttgcagg ttggtcaaat ccatttacag ctttacaact
    13921 tttgtttata aacttagtaa atgactcttt accagcaatt gcattagggt ttgaaaagtc
    13981 agaaaaaaat ataatgaata aacctcctag acacccggat gaaagtatac tagcaggagg
    14041 aacattgaag gcagttgcta taagaggttg tataataggt gtagttacaa taattgcaca
    14101 atatatagga ttacaagtat ctccagaact tggagtagca atggcatttt caacattgat
    14161 attagctaga ataatgcaaa cattacctgc acgttctaat aatcaaacag taataaaatt
    14221 aggttttttc agcaataaat acgttatagg agcagtttta gtctgtcttg ggctttactc
    14281 tttaacacta ataccagtgc taagaggaat attctctata ccaacaacgt ttggattgta
    14341 ccagttgggg atttgcctag ggttagcatt aatttcatca atattaatgg aacttaacaa
    14401 attaataaat agaagtgaag taaaataatc aaaaaattaa agggcatatt gattttaaca
    14461 atatgccttt tataaatagt ctaatttatt ctatattatc gattatttgg aatgctttta
    14521 taacttcagg taattctttt tcttcaggca ttctaaatct tatgaaattt ttatcaagtc
    14581 cagtaaaatc acttccagat ataactctta tttttaattt agcaaactct tgttgtaaat
    14641 ctatgtcctt gtttttatgt tcaactgtca taattgatac agtatcacta gtttcagcta
    14701 tattaaggtt tttccatgga attagtaatt gattttttat atcttttgtt ttttctttta
    14761 attcttctat aaattgaaca tcatctaata aatttgctgc aatactacga gatatttcag
    14821 acacttcgta aggagtagaa atctttttaa tagggctaac aagttgttct ggaagaactg
    14881 catatcctgc tcttagtcct gctaaaccaa atccttttga aaatgttttt aaagcaatta
    14941 cattatcata attatttaga agcttgacag ctgaattttc ttttggcatg tattcaccat
    15001 aagcttcatc taccataact gctatatcat atttagctgc ttcctttact atattttcaa
    15061 ttgatgataa aggtataatt tgtcctgtag gattgtttgg attgtctatg taaattactt
    15121 tatattctgg atttattttt tcaataaatt ctttttcatt aaacttgaaa ttatcctctt
    15181 tctttaaaag aacataatca taagttgcac catgcatttt tatatctgtc tcatattctg
    15241 agaactgagg tgaatatcct aaaactttat ctcccttttc aataaatagt ctatttaaaa
    15301 ggtaaataac atgtatagaa ccatctgcca aacaaattct gtcagtatct aatgctataa
    15361 aatctttcca ataatcgact atattatctt ttaaagcaat tgaatgagga tatcctctta
    15421 tcatatcaaa ttttaaagca ttaaaagctt ctacagcttt gcttgagtaa gacacggtat
    15481 ttataccttc accacagtct atctcgaata catcttctga atgtccagat tctattgcat
    15541 aacttttttt caactgcatt tcaatagctt tgttaatttt tagtttcata ctttatcact
    15601 ccttatttag tcaataataa atgttataaa agttgctaga tttaaaatat tatgaaaata
    15661 tataatagta aaaaatgaat ataaatatta taatagctca ctttatttaa tatgattata
    15721 tatcatatat tgtaaaattt ctagcattat aagactataa attgaaaata ttcagaaaaa
    15781 tactacaaga aacgatttat tttgctctta aaataagtta aatgaatgtt attaattttg
    15841 taaattgaaa tagtttaagt ataaagttat gatataatct aataagggta attctttaga
    15901 ttatattaag gaaatatagg tatacaataa aaagttatgg agttggatta tgggatataa
    15961 attaattgta acagatatgg atggaacttt attaggcaat aatcataaag ttacagatga
    16021 aaataaaact gcactacaaa aagttataaa aagcggtatt aatgtaactt tagcaacagg
    16081 aagagccttc gattcagcta aatgcaatgt agattttctt aaagaggaca tgccaataat
    16141 agcttgtaat ggttcattaa taagagagca aaatggaaat ataatatatt caaataagat
    16201 tgatacaaga acatgtttaa acattttaga tgtattagat aaatacgata tttactacca
    16261 atgtaatagt atagattcta tgctttcgaa aaaaatagaa ggacgtgaag atagattaag
    16321 tgtattttta ggctctgaaa cagaagtaat tgtaaaagat gatttgagag aagaaatctt
    16381 taaaaaagat atactaaaat ttgtaatcat agaagaaaaa aatccatcta ttttggatga
    16441 aataagaaaa gaattgagaa aagttcaagg aataaagata acttcttcat ggccaaacaa
    16501 tattgaagtc atgaatgaag gtgttgataa aggtaatgca gttaaaattt tagctgaaaa
    16561 aatgaatata gacagagaag atataattgc atttggagac aattacaatg acatagaaat
    16621 gataaagttt gctggtcttg gggtagctat gggaaatgca gaagaattga ttaaacaaga
    16681 agctgattat gttacagata caaatcaaga cagtggagtt gcaaaggcaa tttataaatt
    16741 cctagagtta aaagaatcac ttagctctta aaacagtata taataggaaa aaataggttg
    16801 actattttgt agatagtaat tataatacaa atagataaaa cataaaaatt tagattcagt
    16861 gaaaaagaga gtagatatag agaaaatctc agcgaattgg gggatggtgt gagcctaatg
    16921 ataacacttt atattgaaaa gaactttgga gaatactact tgaaattcag tatggtagta
    16981 ggtaatctcg ccttaagaga atagagtggg taaacattgt ttaccaacta gagtggtaac
    17041 acggaatatt tcgtctcttg gtctttttga ccaagagatt ttttagtttc attgagttac
    17101 aagacttata attaaaatac aaaaaattaa aatatgaggt gaaagttatg aaattatggg
    17161 gcggacgctt tagaaaagcg gaaaatcaac ttatggaaga atttaataaa tcttttggct
    17221 atgattgtgt actgtacaaa aaagatatcg aaggaagtgt agcacatgtt catatgcaag
    17281 taaaatgtgg acttttaaca gaagaagaag gaaagtctat aacagaaggt ttaaaaggga
    17341 tacttgaaga tgtagaaaat ggaaagttag tattggatgg agagtatgaa gatatacata
    17401 gttttactga gataaattta attcaaagaa ttggagatgt tggcaagaag cttcatacag
    17461 cacgtagtag aaatgaccaa gttgcagttg atatgagatt gtatgctaaa gaaaaagcaa
    17521 atgatttggt gggtttaata tctgaattta aagctactat aaaagatgtt gctgacaaaa
    17581 acccagtgat gatgccaggc tatactcatc ttcaaagagc tcaagtagtg acatttaagc
    17641 atcacttaat ggcatattat agtatgtttg atagagatga gaaaagaata aaaaatgcta
    17701 tagaaatatt agatgaatca cctttaggct gtggagcttt agcaggaact acgcatgata
    17761 tagatagaag tataacttgt gaacaattag gatttaaaaa agtagtagat aattttatgg
    17821 atggagtaag tgatagagat tatttactag agctaatgag tgatttttca attataatga
    17881 tgcacttaag tagattaagt gaagagttga tactttggag ttctcaagaa tttggatttg
    17941 ttgagataga tgatttatat acaactggaa gtagtataat gcctcagaag aaaaatccag
    18001 atggtgcaga gcttataaga ggtaagactg gcagagtata tggaaactta tttggtcttt
    18061 ttacagtaat gaaaggaata ccattagcat ataataaaga catgcaagaa gataaagaag
    18121 gttttttcga tagtgtacat actcttgaaa tgtgtataca aataatggat agaatgatag
    18181 ccacactaaa ggtaaatgag gataaaatga aacaagcagt aaaaaatggt tttttaaatg
    18241 caacagaagt tgcggattat ttggttaaaa ataatgttgc ttttagagat gcacatggta
    18301 tagtaggaag tatcgttata tattgtgaag ataataaaaa agcaatagaa gatttaacat
    18361 tagaagaatt acataagttt tcagatgctt ttaaggaaga tatttatgat tttatcgact
    18421 atgagagtat attaaataag ggaataaaaa agaatttaaa ataatgtatt tatttttaaa
    18481 agctgtaaat ttgaatgtta tcatttaaat ttacagcttt ttattgtgta agattttatt
    18541 atataaaagt aagaaactta aaaggagtag tatttaaaat aaatacaaat aaaaaattat
    18601 tattaaaatg ataattaata tcaataagta ttgacaaaat tatttctaaa tactattata
    18661 tataatataa ataataatga aaatcaatat caaataaatg ggaggaaatc taatgaactt
    18721 attagtgatt ggtggtcatg aaagaatgga aaaagattat atggttatgg ccaagaaaaa
    18781 aggttataag acaaaggtat atactaccat gtcatctaaa ttaaataatt ctataggaag
    18841 accagatgca gttgtcatac ttacatcaac tgtttcacat aagatgtcta gaacggttga
    18901 gtctcaagct aaaaaacaag atatattaat cgtaaggcat aaaaatagta gtaaggtagc
    18961 atttaatgaa tgtctagata tggttgatga atgtctggga aattgttcaa aatgtaagct
    19021 taaagacaaa caaaaacaaa aaaatatata ggtataaaaa atacataaac ataaaattaa
    19081 aagtactgag ataatagtta taattattat ctcagtactt ttagatttat aaggataagt
    19141 atattataaa ttttttatta taatttccaa ctacttgtta ctttttcata ttttttttac
    19201 ttctagcttt ttttcttttt ttcttcttta tatttgaagc tattataaat aaaactaaaa
    19261 gcactagaat acctgcaata atcttaacaa aagtcattaa aatattattt ccagtaaaaa
    19321 atggaagact gctatcaaga ttattttttg caattaagtc tatactgttt tctagtttac
    19381 cattattata tacatttaaa gtaccaacct tagctccttt ttttataggc aaatcaattt
    19441 tatttaactc tgttttggta tcataattgc cttgagattc atctttttct aagaatatcc
    19501 tataactatt ttttggttca taaatcaatt catgttgttt tgtaaacaat acttttttag
    19561 tatctgtgta tttttcttta tcaacaatag tagcacactt aaagttatca aatccataat
    19621 caatcagaat acgtgagtct aaatataaat cagcattagt tgaattaaat acagcaacga
    19681 taactcttct gccatctttt acagcactcg ataaaagaca tttaccagca gcatcagtat
    19741 atccagtttt tataccatca actatatcat attttatatt tataggttga cctttgtatg
    19801 taatcttatc atgtgatgtc aaaaacttat tagtgtttac aaagtatctc tcataaggat
    19861 atgcttttgt agcttcaaat ttaagtgact tagtttttac aatttgtcta aaagtatcat
    19921 tattcatagc ttctctagct atgagagcca tatcatgagc tgttgtgtag tgatttgggt
    19981 ctggtaatcc atgaggatta ttaaaatgag tattagtagc tccaattttt tttgcctcag
    20041 agttcatgag gtcaataaat ttttgtacat tgccaccacc aacatatcta gccaatacaa
    20101 aagcagcatc attagcagaa tgaaccaaaa gagcatctag taactgcttt acagtaaaag
    20161 attccccatt ttttagatac atactagagc catctatttg aggtaaatct ttaatttcta
    20221 ttacttgatt taaatcttta acctccttta gaactaaaca agctgtccat actttagttg
    20281 ttgaagctgg ataaagctta gaatttccat ccttattata tagtattttg ccagactcat
    20341 agtccattaa aactgcatgt ttagcaacta tagctggttc attatctgca aaagagatat
    20401 tagagcttcc taaaaatgaa gtaaatatca ataaacagat taataaaagt gataaatttc
    20461 ttttcattgt gagcctccaa attttaaatt catcttttag tatagcatat atttgtcaat
    20521 aattaaaaga aaggagtgat taatatatga aaaaaaaggt aaaatttatt tcgttttttg
    20581 ttttatttat tttaataata tcatcaataa tcgtttataa atcgcttatt ttcaaagatg
    20641 atatttatgt tgtaagtaaa aaaaatttac ctaatgaaaa aacacatgtt gtagagaatg
    20701 aaattaaagg tgaagataat aatcacaata aagtaactaa agacatagat aaaaattttc
    20761 aaaataataa aataactata tacataagtg gagcagtaaa tagacctgga attgtaacta
    20821 tagagtcaga caagagactt tatgatgctg tagaattact aggaggaacg acaaaagaag
    20881 cagatttaaa tggagtcaat ctatcagtaa gattagaaga tgaacaacat tacataatac
    20941 ctaaaatagg tgaagcaaca tctgttacaa gtaatgatga ttccaataaa ccaaatcaga
    21001 agaatgagtc aagaggtgag cctaaaaata aatccaataa aaatatatct aacgaatcaa
    21061 aagtaaatat aaatgtagcg actatagaag agttagattc tctacctggg gttggagaag
    21121 ctacagccaa taaaatttta caacacagag aagaaaatgg acagtttagt tcaattgagg
    21181 aaataaaaaa tgtaaatggt attggagata aaaagtatga aaatataaaa gatttaattt
    21241 gtgtagacta aaatataata caaaacttac atatagatat gttattataa atatgtatgt
    21301 atatgtgtgt acatgtggaa atttagtaaa ggaggcactt ttatgaaaaa agaacaaaaa
    21361 aaacttacag agatgacaac agcaggtggt tgagccgcta aaatagggcc agaggttctg
    21421 gcatcagttt tatctcaatt gcctaaaaat gataatatag aaaatttatt agtaggacta
    21481 gatacagcag atgatgctgc agtttataaa ttaaatgatg atatggcact tatccaaact
    21541 ttagacttct ttacaccaat ggtagataac ccatacgtat ttggtcaaat tgctgcatca
    21601 aattcactat cagatgtata tgctatggga ggcaaacctt tggttgctat gaatattgtt
    21661 tgttttccat catgccatga tatggatgta cttgcggaaa tattaaaagg tgggtttgat
    21721 aaagtgaaag aaagtggagc acttttggtt ggagggcata ctgtagatga caaagagcct
    21781 aaatatggtc tttcagtttc tgggattgta agtccaaata aagttttatc aaatgctact
    21841 gcaaaacctg gtgataagtt aattataaca aaaccaattg gagtgggtgt attaaataca
    21901 gccatgaaag aaggtatggt agaacaacat atagcagata aagtaataga aataatgata
    21961 catttaaata aatatgcagc aatgagtttt gagaaatttg atgtaaattc ggttacagat
    22021 ataactggtt ttggtctact tggtcatact ttagaaatgg ccaaagcttc tgaagtaagt
    22081 attgaaattg aatcaaaaca tgtaccaatt attgaaggtg caatagaaat ggcacaaatg
    22141 ggaattatac ctgctggtat gtataaaaat atgcattatg tatctaaaga tgtagaagtt
    22201 gtaggaaata tagaagttgc agttcaagat attttatacg acccacaaac atcaggaggg
    22261 ttgttaattt cagtaaaaga agaacttgca gaggaacttg taaaagatat gaaattaaat
    22321 ggagcaatag aagctaaaat tataggttct gtagttccaa aaggggaaaa atatattaca
    22381 gttttataaa atattataga gaaagagtga ttgttttgag caaaagagaa ttgtttgcaa
    22441 tgttgccttc agttgatgaa gtattaagtg ataatagaat tgtagaaata ataaatgaat
    22501 atcctagaag tttagtttta gaatctgtga gagaagtaat agatttaaaa agacaattta
    22561 tattaagatt aaaagaagat gcatcaaact ctgtaacaat agagtttggt gaaataatag
    22621 aatctgcaat agagagagtt aagttaaatt actcattatc tttaaaaaaa gttataaatg
    22681 caactggtac agttattcat acaaatttag ggaggtcttt acttagtgaa gatataaaag
    22741 atgaattatg gtgtgctgct tcaaggtatt caaacttaga atacgatttg gataatggag
    22801 aaagaggttc aagatactct catttaacta gtacaataaa aagacttact ggagcagaag
    22861 atgtactagt tgtaaataat aatgcagcgg cagtacttct tgtacttagc acaatggcta
    22921 aaggaggaga agcaattgta tctcgtggag aattagttga agtaggtggt tcatttagga
    22981 ttccaagtat aatggctctt agtggtgcag aattagttga agttggttca actaataaaa
    23041 ctcatttgaa agattacaag gaggccatta cagaagatac aaatgtattg atgaaggttc
    23101 atacaagtaa ttatagaata atgggattca cagagagtgt ttctatagaa gaattggtta
    23161 atttaggtaa aaaatataaa ctacctgtaa tagaagactt aggaagtgga gtttttatag
    23221 atttatcaaa atacggttta tcttatgaac caactgtatt ggattctatt cgacaaggtg
    23281 cagatgtagt aacttttagt ggagataaga tgcttggagg tccacaagca ggtattatag
    23341 ttgggaaaaa agagtatata gaaaaaatga agaaaaatca acttactaga gcacttagag
    23401 ttgataagct tacaatatgt gctcttgaag caacattgag aatgtattta gatgagacaa
    23461 aagctataga aaatatacct actttaaaaa tgttgacata taaaattgaa gaattagaag
    23521 taaaagcaaa taaactattt gaaaaaataa ctgctttaaa cttaaatgca aacataaata
    23581 tagaagatgg attttcacaa gtgggaggag ggtctatgcc tcttgaaact ataagtacaa
    23641 aggtaatatc aataactcct gaacatatga atgtttcatc tttagaaaaa aaactaagac
    23701 ttagtgaagc acatataata gccagagtgt atgataataa atatgtacta gatgtaagaa
    23761 ctatatttga tgatgaattt gatgtaatag ttgcggaatt gagaaaagca tttaattaaa
    23821 taaatttgag ctaataaata ttaaatttag aagtataagt atgaagtttt gattaaaaaa
    23881 tagctacaca cacttgtgac tttagtcata gcaggtttag agggggcaaa aaatgaaaaa
    23941 tgtagtaatt ggaacagcag gtcatattga ccacggaaaa actactctta taaaagcatt
    24001 gactggaaga gagactgata cattagatga agaaaaaaag agaggtattt caataaactt
    24061 aggatttact tattttgatt taccaaacaa tagaagagct ggaattgttg atgtcccagg
    24121 acacgaaaaa ttcataaaaa acatgttagc tggagcatca ggaattgata tagttttact
    24181 tgttgtagca gcagatgaag gggttatgcc acagacagtt gagcatttag atattctaag
    24241 ctttttaaat atcaaaaatg gaatagtggt tctaactaag tctgatactg tagatgatga
    24301 atttagagag ctagtaaaag aagatatttg tgaaaaaata aaaggaactt ttcttgaaga
    24361 tgctgaaata attgaagttg attctatatc taaaagaggt atagacactc ttattgaaaa
    24421 aatagataaa atgagtgatg atatagaaga taaaaatgaa aactctccag caagacttaa
    24481 tatagacaga gtattttcaa taaaagggtt tggtactgta ataacaggca ctttaataga
    24541 aggaaagata agtatagaag atgatttagt tatatatcca caggtattaa aaacaaaaat
    24601 aagaagtata caagttcatg gagaaaataa agatacagct tatgcaggtc aaagaacagc
    24661 aatcaatata tcaaatgtaa aagtggatga tataaaaaga ggagatgttt tagctgcacc
    24721 aaactcatta gaggaatcaa tgatgttaga tgtaaaatta tcattggtaa atcatttaga
    24781 taaaggatta aagcactggg atagattaag attgtatcat ggaactagag agatactttg
    24841 tagagcagtt ccactagata aagaattaat tgaaagtgga gaaagtggct atgttcagtt
    24901 gagacttgaa gaaagcatag tatctaagaa aggtgatacg tttgtagtga gacgttattc
    24961 tccaatggaa actataggtg gaggtgtaat aattgaccca tctcctaaaa aacataagaa
    25021 atttgatgaa aaagttatag aagcattaaa aataaaagaa aaaggcgaac taaaagatat
    25081 aatagaagag tacttaaaaa ggaatttaaa aaattatcca aatataaaag aaataatgag
    25141 ctatagtggt gctcatgaag aagatgtaaa aagagcgctt gaagcactta tatcagaaga
    25201 taaagtattt attataggaa atatgtatat gcatataaat caatacaata aattaaaaga
    25261 aaatacaata aagcttttaa gtgagtacca taagaagtat aggcttagaa aagggatact
    25321 aaaagaagaa gtaaggtcta agatagagag taattttaag actagggaga tggatatttt
    25381 attagaaaaa ttgtctacag aaaatgctat aaaaatagaa aataatatag tatctctatt
    25441 agattttgag gttattttaa atgataagca aaaagaaata gcaaaaaaaa tagaaaaaag
    25501 attaaaatca tgtggagttt cttctatact gacaatagat gaagtttctg aaggaaatca
    25561 taactatgct gaagtattgg agtcaatgat aggtaataaa gtagagaaat tagatgattt
    25621 gtatataatg gacaaagata tttatgaaaa tgcaaagaac attttgataa attatataaa
    25681 agaaaataaa gaaataacat taggagaata cagagatttg attgattcta gcagaaagaa
    25741 ttgcatgatt atattggaaa acttcgatag aaacaaaata actaaaagag tagaaaacaa
    25801 aagaattttg ttttagcaga aaatgtctgt ttttgcttta aggtataaag aaggaattat
    25861 aatgtcgagt atcgtataat taattagtat aaaatattaa ttaagaggaa gtgtaaagta
    25921 ttgaagaata taatacctga tgtaaaacca gatataaaag aaagagaaga catgagcctt
    25981 ttatttataa taatgggctc agtactttta ttatttatta taaaagatat agagaaatat
    26041 gaaactttat tacatgccat atatacgata tttttagtag ctacaggagt aattatattt
    26101 aatactacaa aatttaaagt agattcattt tctacatttt taggattaat ttttatagct
    26161 actggagtat tggagtgtat atatttattt aattccttgg ggattaaaac taagtctata
    26221 atggagatta acatcacaat atcagcaata acagatttat ttcctatact gggtgtatac
    26281 ttaagtttta aatttgttaa ggataacaaa caaatttatt caagtataat tttatttata
    26341 gcaactgcta cattaacaat aacatcttta ttcataatat caggaatttg tgactatctg
    26401 ggaaatggta aatcacaata tgctttagga gttataatat ctatattcat aataattatt
    26461 tctatcatta gtggaattga attaaattca agttcaaata aaggtaaatg ggaatatgat
    26521 gaaaaaaaga tttttaacag aataatagta ataataattt tatcaagagt tccaaatcta
    26581 ttgcatatag ttattgataa tagaaatgta gagcggatat tatcacagat aataataaat
    26641 gtagccttat attatttata taattacata gtatctaaga atattaagaa aacagcactg
    26701 gaattgcatg atacgaatga agaattaaca aaaaaaacgg aaagtttaaa agaaaagaat
    26761 aaaaaattaa tctatgagac agaaaagata gaggaactta agggaatact tatgaaaaga
    26821 gaatctaggc tacaatctac attagatgta gcagtaaata gcatagtagt gtttagcaaa
    26881 gatggagaaa ttacatatgc aaataagagc tttagaaata cttttggaga ctataaagaa
    26941 caagataaat tgactgataa aataaaaaac ttcaatgatt tgatagaaag cattcattat
    27001 gtgtttgttc atgagaagaa tgcagagaaa cttattagta caagcgataa taaagtttat
    27061 caagctatat ttgcaccttt aattattgct tcacaaacag aaggagtatt gtgtgtacta
    27121 gaagataaga ctaagaaaaa ggagtacgaa cgcaaactta ttgaagcaaa taagagaagt
    27181 aaagattttt tagaaagtat tggagatggc atagtagtgc ttgaaggtaa taacaaaata
    27241 tatgttaata atgcatgtag ggaaattttt aaaaataatt tagagagtat tgatttttca
    27301 ttagtatgta aaagtgaaga aagtatagaa aaaagatatg taatagatgg tgaagtaaag
    27361 tatgtagaga tgagtttttc tcagtatact aatgctggta ctaataaaac tattattgta
    27421 atacgtgata caacctctcg aaaaatatct caaataaaac ttgaggaaag tcaatcatca
    27481 tattcaagat ttatagatat attacctgat ggcatatgct tattaaaaga agatttaagt
    27541 ataaactatg caaataagtc gtttttagat atgctagctt ttacaaacat agatgatata
    27601 aaagattcta atattaaact attaatgaat gcgaatactg aagaaaaaat gaaatttacg
    27661 gataatatgg ctaaagtttt aaatgaaaat agaagtatac ttttactgga atatgaattg
    27721 ataaatagtt atgatgatat agtagaagta gaattaagtg cattaccttt tgctatatac
    27781 aatactagat acataatgct tattataaaa gatttagtac ataagaaata ttccgagcaa
    27841 gcagaaaaag aactgcttga aagatttaaa acagataaaa tcaaaacgga gttttttgca
    27901 aatatgtctc atgaattaaa aactccacta aatgttatat ctagttcaaa tcaactggta
    27961 gattctttct atagaaatga aaagataaaa gattacaata acaatattaa atctcatgtg
    28021 gatttagtta gacaaagttc ttataggctt caaagattaa taaataacat tatagattta
    28081 acaaagatgg agtctgggtt ctatacacta aaacttgcga aatacaatat tgtatctgta
    28141 atagaagact tatttatgaa tatagaagag tatgcactta gaaaagacat aaaaattctt
    28201 tttgatacag accttgaaga aattaatgtt tatatagaca aggttgaaat tgagagaatt
    28261 atgttgaatc tattgtcaaa ctgtattaag tttactgata atggaggctg gatatatgta
    28321 agtattcatt ataaaataga taaagtaatc ataagtgtaa aagacactgg agttggaata
    28381 ccacaagata aattagaatt gatttttgaa gaattttcac aagttgataa gactttatcc
    28441 agaaatactg aaggaagtgg aatagggctt gctatagtta agaatctagt aagtcttcat
    28501 ggtggagata tagaagtagt aagtgaagtg aataaaggca cagagttctt aatttcactt
    28561 ccaatgaagg gatttagtag tgaacattac acagaagata agagaatata taatattcaa
    28621 gaaaaaataa aaatagaatt ttctgatata tactattaaa aatgattatg agcaaaaata
    28681 aaataaaaat actatttgtt taaatattta agttgtaaga aataggctat cataaggtaa
    28741 attattatga tagcctattt ttagctaggt tttatatcta tatgtgagaa catataatct
    28801 ctaaattacc ttcaaaacta aaatctccaa agtcgtttgg cagtataaaa tgcataccct
    28861 tttttaaatt atattctaca gaattgtgta taagttttcc atttccatcc aacacactgt
    28921 ataaagaaaa aggtgtattg tgtgtaaaat tacattttcc aaaaacatct aatttataaa
    28981 cactaaaaaa ttcgtttgac acaaatgttg tgcatttaaa gtctgatgtt gatacgattt
    29041 tatagtcagt atcgaaattt atatgaggaa cattcgtaac atctattgat ttttgaacat
    29101 gaagttcacg cttattacct gaattgtcag ttctatcata atcatagact ctataagtag
    29161 tatctgagtt ttgttgagtt tctaaaatca atgttccttt gcatatagca tgtatagtac
    29221 cacttggaac atagaaaaag tctccctttt ttatattaac ctttctaagt agtttgtccc
    29281 attcattatt atttaccata tttacaaatt ctttatgaga ttttgcatta tgacctatta
    29341 taatttctgc atcatcagaa caatctatta cataccaaca ctctgtctta cctaactctc
    29401 cattttcatt tttcttagca tattcatcat ttggatggac ttgaactgat aaattatcat
    29461 ttgcatccaa tatttttgta agtaaaggaa acttatcacc aggtgtgttt ccaaataatt
    29521 ctctattttt attccacaat tcagatagtt ttttaccttt atattttcca ttttctatta
    29581 agcaatctcc atttttatga gagcttatag cccaacattc accagttgtt ggcgaatcta
    29641 tttcataatt aaatttatct ttaagtgcag taccacccca tattctatcc ataaaaatag
    29701 gttttaaaaa taatggttcc ataatcctgt tcctcctatg atattaaatt tatcctacaa
    29761 ataattatat ataaatagat tataaaatca atagtaaaaa agttttgtcc cttgaaatga
    29821 cataaagtat aaggagttat taaataagat aaactatgat atataatata aagttctaaa
    29881 ttggagtgat aattatggag ttgtttaagg cagaaagcaa aaaatatgaa aaagaatttt
    29941 taaaattatt ggagcaatgg gttagtatac cttcatttta tgatagaaaa actgtatcta
    30001 aagatatgcc ttttggaaaa ggagtatatg atgcattaaa ctggtttgaa aatctaggta
    30061 gagaaaacaa tttcaaagta aaaaatatag ataatcatgc tgttcaaatt gaatatggaa
    30121 atggtaaaga atatgtagat atatttggac attgtgatgt tgtaaatcca ggtgaaggat
    30181 gggatagtga accatttaaa ctaaatatta taggtgataa attagttgct agaggtgtat
    30241 ctgataataa aggacctatg atagtgaatt ttttagcact aaaaatgata aaggatttag
    30301 atattaattt aaaaagaaag gttagattaa tagctggagg aaatgaagag agtggattta
    30361 aatgtataaa gcattattat agcaaagagc catatggtgt atgtggattt actccagatg
    30421 ctaaatttcc tgtattaaat ggagaaaaag gtggagcaat aataaagtta atttcaaata
    30481 tagacgataa atctttatat ataagtggtg gaatagagtt taatacaata ccagataaag
    30541 tttatataaa gaatgtagaa aaattgggta aagataatat atgttttgat attaacaata
    30601 tatcgataaa ttatgataat ggcaattata ttgtgtatgg aaaaggtggt cattcatcta
    30661 aacccgaaaa atcaataaat cctatattag ctacaattaa acttttgtca gaaaatattg
    30721 atgaaaaatg gacaaaagat ttatataagt tgataaatca agataatata aatggaaact
    30781 tatttggttt aaatattgag gggaaatgtg ggattttatc aatagtgcca acaataataa
    30841 atatagtaga tggaaaatta gaagtagttt taagcgttag atatccagaa atattaacca
    30901 ttgaagatat aataaaaaaa tttaatttgt atatggagca aaataatatt aataaatttg
    30961 agcttattgg ggaaaattta aagcaagcaa attatataga tagaaattca aaacttgtaa
    31021 gaagtttgca tgatatatat ataaagtatt ctggagattt gaaaaatgat gttagagtaa
    31081 ctagtgcagg aagttatgct tctgaaatga ataattcagt aatatttggt tgtgaatttc
    31141 ctgatggtag ctttggcaat gtccatagtg cgaatgaatt tgctagctta gatagattta
    31201 taactgcaat aggtatatat gcagaggcaa taattacatt atgtaataaa atataaatac
    31261 aaaatgtccc ttatatagac atattatata tacaattaaa attttacttg atatataatt
    31321 gaaatcaaag agaaggtgat acaatgataa ctaagaaaca tgcaataata gtaaagagtt
    31381 taagcaataa agatggatat atgacatcaa acgaattagc tatcaaattg gatgtgtcta
    31441 ctaaaactat taaaagatat atagcagatt taaatagtgt tttaagcaaa tatgatttgg
    31501 taatagcttc ttctagaggt ataggataca agttaagcgg ttctaaaaac aatatagcca
    31561 gagctgttaa agaagcaaat aaatatattg atggtttttt agatgactct gaagagtcaa
    31621 gaatgtcaaa tataatatgt atgcttatta atagaaatta tatgagtata gaagcaatgg
    31681 cagaagaatt aaatctaagt atagccgcta taaataaatt gtctagtaag cttaaaagaa
    31741 aacttgaaaa gtatgactta gttataaaat ctaaacctta ctatggaagc tatatacatg
    31801 gtgaagaaat taatataagg caattaatta ctgactatgc aataaaatta gatgaaaaaa
    31861 gtaaagtgaa ggtgttttta gatgacatta gtgaaaacga tatatactgt atagaaagta
    31921 ttttagaaaa gcacttaaga gaaaaagata caataatttc agataaagat tttaatcttt
    31981 tgctttcaaa aataattgtt tctgtattta ggagtaagag aggtcatagt aacaatataa
    32041 acttaatgga tacaagttat agatttcata actactactt tatagaaaat ctaatgaaag
    32101 aaatatctga taaaattgga tttaaactta ttgaagatga agtaatttat atttcaaatt
    32161 attctggtgt aatagcttat aaaggtacac aaggaagaaa aaacacaagt gaaattgagg
    32221 aacgaataag tagtgttata agtagtgcac ttcaagatat attccttatt tcaggtagtg
    32281 attatacaaa agatgatgag tttatggttg caatctctga ccatataaag agatttttaa
    32341 atagagctag agctaatgta aaatcaaata atcctttact tcatcaaata aaagagaagt
    32401 ttcctatagc ttttaattta gccgtgtttt tatcaaataa actagaaact gagtttaatt
    32461 taaaacttga tgaagatgag cttggatata tagctatcca ctttgctgct tcaaatgaaa
    32521 gaatgaaaaa aaatactagt aagaaaattt gtatagtttg tcattatggg ataggaacag
    32581 gacaacttat ttctgaaaag ctaaaacaga atataagtga tttaagtgtt gtaggtgttt
    32641 atccagttag atatcttgat atggctataa gtcaagatgt tgatttgata gtatctaccg
    32701 ttgaattaaa aggatatgaa aaacctgtct tatatataga aaatatattt gatgatagct
    32761 taattgaaaa cgtaaacaaa gccttttatg aaaaggaaga aagaagaaag ataataagta
    32821 acatgtttga tgaaaaagct tttttcagta taaaggctag tactaaagaa gaggtcataa
    32881 tttcactttc aaataaacta aaagaaagag attttataga ggaaagttct ataaaaagta
    32941 taatagatag agaaaatatt tcatcaactg aaataggaaa tctagtagca atacctcaca
    33001 caatagtgaa gggagataaa aaatctataa taggagttgg aatacttgaa aatccaataa
    33061 tttgggataa gcaagaggtt cagctagtat ttatggtatt ttttaatact aaagaaaagc
    33121 ataatttctc gatatttaaa tacctttata attttataaa agatgaagga ggagtaagag
    33181 gaattattaa aatatgtgac tttaataaat taatggcact gataggtaat taaggaggaa
    33241 attaataatg ggaacaaaca tatttaacaa agaatatgta tttttaaatg ttgatgctaa
    33301 gtcaaaggtt gaagttttaa aatttatatc aaaaaaagct aaagacttaa atttggcaga
    33361 agatgagagc ttagtatatg aaggtttaat ggctagagaa gaacaattta ctacaaattt
    33421 aggagaatct atagcaatac ctcacactaa aaatgatgca attgaaaatc cagcagtagt
    33481 agtattgaag tttaatgaag atgttgtttg gaatgaaggt gaggataagg ttaaattagc
    33541 aataagctta cttatgccag gaaaaagtaa agagaatatt catcttaaat tattatcatc
    33601 tctttcaaga aaattaataa ataaagaatt taaagattct cttttaaaaa gtgataatgt
    33661 tgaagaaata tctaacttaa taaatgaagc tttaggttta taattaaata ctaaggttat
    33721 aaaataaaaa aataattaac taaaaatagg aggaatttga aatgaataaa aaattagtag
    33781 cattatgtgc ttgcccaatg ggactagctc acactttcat ggcagcagaa gcaatagagc
    33841 aagcagcaaa ggcattaggt tatgaagcaa aagttgaaac tcaaggtgct gatggtgtac
    33901 aaaatgagtt aacaagagat gatatattag gagcaactat gataatacat gcagtagcaa
    33961 taacaccaga aggtatggaa agatttgatg gttgtgaagt ttatgaagtt gaattacaag
    34021 aagctataaa aaatgcagaa ggtgttataa aagaaataga agaagattta ggaatataat
    34081 aaatttattg tggaggaaat agaatggcaa ttaagaaaaa agttataaac agttcaggtg
    34141 cttctacaac aggaggaaat aatgtaggaa aatctactcc atctagtcca actcaatcaa
    34201 agggaaatgg aaaatggaaa gaaataagta aacatataat gacaggtata tcatatatga
    34261 taccagtttt agtaatgggt ggacttatag gggccttatc tcaattaata ccatatgcta
    34321 tattaggact tgacccatct gtaggtatag ttgatgctat gaactcagga gaatttacag
    34381 ggttcaagct tagtctttta aatatagcac aattaatgtc aaatttcgga tttactttgt
    34441 ttggatttgc gataccactg tttgcagcat tttgtgcaaa ctctataggt ggaaaaacag
    34501 ctttaattgc aggatttata ggtggatata tagctaataa acctgtaggt gtaccacaat
    34561 tcattgatgg acaatggact gaagttgttc cagttgcatc aggattttta ggtgcaatac
    34621 taatagcatt cataatagga tattttgtaa aatggttaaa taaatctata aaagtttctc
    34681 ataactggtt agcttttaaa acaacatttt taataccatt aatagcgtca ttagcttgta
    34741 tggtattgat gatatttata ataactccat ttggtggatt aattaatgaa agtatgaaaa
    34801 acttcttaac agcagcagga gcagctggag aatatgtata tgcaacagct ttggcagcag
    34861 ctacagcatt cgacttagga ggaccaataa ataaagcagc aggattcgtt gcacttggtt
    34921 taactacaga gaatgtactt ccaataacag caagaacaat agctatagtt gttcctccaa
    34981 ttggattagg attaacaaca ttattagata aaagattagt aggaagaaga gtatatgata
    35041 gacaattcta tcaagcagga aaaacttcta tattcttatc atttatgggt atatctgaag
    35101 gagcaatacc atttgcactt gagagaccag gttttgttat acctttaaac atagttggaa
    35161 gtgtaatagg agctattaca ggtataattc taggagcaat tcaatggttc ccagagtcag
    35221 ctatatgggc atggcctcta gtagataact tatttggata tataataggt atagcagttg
    35281 gtgctatatt catagcagta ggaaatatat tctatagaaa taaattaata aaagatggta
    35341 aacttgttgt agattatatc gactagtatt taatttttac tatctataaa taactttggc
    35401 tggtaatatg gttttaatca atattaccag ccttacctat atgattaatt aaaggcggtg
    35461 gagataagat atggataaga tagaaaaagt aagagagtat tttagagaat atgatataga
    35521 tggatttctt ataaatagtc ctacaaacaa gttttatgtt ggaaatttat ttagctcttc
    35581 aggttatgtt tttataacga aagaatcaca atatataata gtagatttta gatattttga
    35641 agaaattaaa agaaaatcta gtttgtttaa tgtagtatta atggacaaaa ctagaactca
    35701 ttttgatata attaatgata tttgtagaga gcagaatata aaggaaattg gatttgaggg
    35761 aaatgaagta tcttttgatt tatatagaag tatgagtaat aagttaagtg caaccttaaa
    35821 atcagttgat ttatctacat taagggaaac aaaaaatgaa gacgaaataa aatatataaa
    35881 aaaagcttgt gaaatagttg atgctacatt ttatcatata gtagacttta taaagattgg
    35941 tatgactgaa aagcaagttg aaaatgaaat tgttagaatt ataaaagatt taggtggtca
    36001 aaaagaatct ttcgatacaa tagttgcatc tgggcttaga ggagcattac ctcatggaaa
    36061 agcaagtgaa aaaattatag agtatggaga ttttgttact tttgactttg gtgctaagta
    36121 taataattat tgttcagata ttaccagaac catttgtatg ggtactataa ataaagagtt
    36181 agaagaaata tataatatag taagaaaagc taatgaagag tgtataagag ttttaagacc
    36241 gggaatgact actggagaga tagataaagt tgcaagagac ataattggtt catatggata
    36301 tgccaataat tttggtcata atctgggtca tggtgtagga attatggttc atgaatatcc
    36361 tgccttagct cctgaatcaa atgaagtatt aaaagaagga atgattgtaa ctatagagcc
    36421 tggtatatat gttccaagtc ttggaggcgt aagaatagaa gatgatgtac tcataacaca
    36481 agatggatgt atgagattga ctacatctac taaggattta atagttgtaa gctaaaaata
    36541 aattagataa atattttata acaataagaa aagaagaggt aaatttaaat gtataatttt
    36601 gatgataaac cagatagagt aagtgaaaag tgtagaaaat gggatttaaa tattataaga
    36661 gataaatttg gagatattag agaagatttt gtaccaatgt ggattgctga tatggatttt
    36721 aaaataccaa cagaaataga aaacaaattt attgaagctg taagaagagg tgtatttgga
    36781 tatacatact gttatgatga attttatgat tcagttattg gttggcaaaa aaatatgcat
    36841 gaggtaaatg tagaaaaaga atggattaca ttaacttatg gaactgtatc aacacttcat
    36901 tatgtagtac aagctttttg taagaaaggt gatagtataa tattaaacac accagtttat
    36961 gacccctttg aatcatcagc aaaaaaacaa ggtgtaaatg taatttgtaa tacattagat
    37021 gttgtaaatg atagatatta tataaatttt gataaattag aagttcaaat taaagaaaat
    37081 aagcctaaat taatgatgtt ttgtacacca cataatccat ctggaaggat atggacaatt
    37141 gaagaaatga caagggtagc aactatatgt aaagaaaata atgttatttt ggttgcagat
    37201 gaagtacatg cagagcatat tcattatggt aaatttaatt caatacttaa gattggaaaa
    37261 gagcttttag aaaatgttat tctactgaca tcaccaaata agggctttaa tcttggtgga
    37321 ttaaagactt cttattcaat agttataaat aagaatataa gggataaatt tagaaataaa
    37381 ttaaagcaaa attctataac ttctccaaat gtgtttggaa taataggatt gataaccgct
    37441 tataatgaat gtcatgagtg gttaagtggt gtaaatgaat atataaaatc aaattatgaa
    37501 cttttagaaa cttgggtaaa taaatataat aagataaaat tgatgaaaat ggaatcatct
    37561 tacttagctt ggatggatat aagtggatta ggtataagtg caagtgagtt tacagataaa
    37621 ttggctatag atacaggagt attattagaa gatgggagtc attttgtaaa agatggtgag
    37681 aagtatgtta gaataaattt aggaactcaa aaggaaaatg taattgaagc tttaaataga
    37741 atggatttat ttttaaagtc tttataaaaa tttggttgaa ttagtatttt atataaattt
    37801 aagctattaa tatggtatat acataagtag aatttgtttt agattactta agtatatacc
    37861 atattttata taaaaattaa atttgcatta gtcatgtgat aactatttaa attcatatgt
    37921 attttgtatg tataatagat ataattatta tttaaactta ttgtttattt aatttttcaa
    37981 tagaaaaaga tttatctgta agtttaaaat tatcttttat acccttagtt aaatatactt
    38041 tcttatcctt agttatgaat atagcatcta cattatcaat ttcattaatt aaatccatac
    38101 ctttttcaag accaagtcca aatgtagaag tggataaggc atcacaatta atagatttat
    38161 cagaaattat tgtaacacta cttagattgt tttcaaaagg ataccctgta aatggattta
    38221 acatatgatg atatattttt ccgtcttttt cgataaatct ttcataaatt ccagaagtaa
    38281 caacagattt atttgtggtt tctatgtttc ctatggattc accattttta ctagtggggt
    38341 cttgaatgcc tattttaaaa ggtttatcat ttttaccatc ttctaaagta aatatatttc
    38401 ctcctaagtt tactaaacct ttttttacgt cctctgattt taaataggct acaattttat
    38461 cagcagcata tcctttagct atggcaccta aatcaatttt catattttct ttggttaatt
    38521 ttatagattt atttttttca tccaatatga tgttcttata atcaataaga ggcagaagtt
    38581 tttgtatctc agaatcactt ggaactttag ctttatctgt tccaatagcc cataaatttg
    38641 ataatggacc aattgttatg tcaaaatatc catctgataa tttggaatat tgtatagctt
    38701 cttttacgac gaaaaatgta tcatcagaaa ctctgacaaa ttttttacca gcattatcat
    38761 ttattttaga aacatcactt cctggtatat gagtgctcat tttgttgtca atatatctta
    38821 aaattgaatc acattcattt aagattttat cacttttgga ttcttttaca ttaaatatag
    38881 taacttcgtt tactgtacca aggtagtagc tagttttact atatttagta ttctggcttg
    38941 aactcctaaa aaatgaaaat aaaattaatg ctaaaatcaa tatcacaaaa ataaatgtga
    39001 tttttttctt attcatggta tattcctcct atgaaattgt gttattattt tattataaca
    39061 taatttcata ggaggacaaa aaatgaagaa aaaagacatt cttcttattt taggcatttt
    39121 ggttgttatt atagcatgtt atgggataat aaatgttata aattctaaaa atgctggaaa
    39181 tattgagatt tatgtagata ataaattgta taaaactgtt tcaataaatg ccaaagaaga
    39241 atttaaaata gagaatagag gggggtataa catagtaaag attcatgata aaggggttga
    39301 gatagtagat gcttcatgcc ctgataaggt ttgcgtacat actggattta taaacaagcc
    39361 aagtcaaagc atagtttgta tacctaatag agtgagcata aaaataaata caaatgaaaa
    39421 gaatgataat caagaagata taatttctaa ataaaataat aaaaataatt gaaaggaatt
    39481 ggtgataatt atggttaact taggaaacga ttgggatgaa ttattgaaag aagaatttga
    39541 aaaagattac tacttaaact taagaaaatt tcttatagaa gaatataaaa cacgccaaat
    39601 tttcccaaat atgcacaata tatatgaagc tttaaaacac acttcataca aagacacaaa
    39661 agtcttaata ttaggacaag acccttatca tggagataat caagctcatg gtttagcttt
    39721 ttcagttcaa ccacaagtga aaactccacc atctctatta aatatgtata aagaattaaa
    39781 agatgattta ggttgtttta tacctaataa tggatattta atgccttggg cagaccaagg
    39841 agtgttactt ttaaatactg ctcttactgt tagagcgcat gaagctaatt ctcataagaa
    39901 caaaggatgg gaaattttca ctgatagagt aatatctatt ttaagtgagc gtgaagaccc
    39961 agttattttt gtactatggg gaagtaatgc acgtaaaaaa gtagagttga ttgatacaag
    40021 taaacattat attttggaag cacctcatcc aagcccattg tcagcaagta aaggcttttt
    40081 tggatgtaaa cacttctcta agataaatga aattttgaaa aagctaggaa aagaacctat
    40141 taattggcag atagaaaata tataaaagta aaaggtaaca aaatgataac aaaacaacaa
    40201 taaacaatga cattgttgta actaatagaa atatgcttaa atagttgaaa atactatgta
    40261 tgcatatttc tatttttttg tcaagttgac aactcttgaa aaaatagtta caatttagtt
    40321 acacaaaaaa cacaaataaa aaaagaaact tacaaagcag aattttatga agcaaaatat
    40381 atagtgtaaa aaattttagg gaggaaattt tatatgaatt ttaaaaaatt gagtaatgct
    40441 aaagtctcgg taatggcagt tgttttatca gttggaatat tatcgggata ctctgcgctt
    40501 aataaacaag taacattagt agtaagggga gaagaaagaa ctatatcaac atttagttct
    40561 aatgtaggag accttttaga atctcaaaac attagctatg atgcaaatga tatagtaact
    40621 ttagatgaaa atacaaagtt atctaatgga gataaaatag aagtaattga tgtaaaggaa
    40681 aaaacagttg tagaaactaa agacattcca tttgaagtta cagttgttga agatggctct
    40741 ttattaaagg gtgattctaa ggttaaagaa gaaggaaagt ctggtaaaag tgaattaata
    40801 tataaagaaa cttatcacaa tggaaagaaa gttgaaaagg aatttgtgaa aaaggttgtt
    40861 aaagctgagc cagtaaataa ggttgtaagc aaagggacaa aggtagaaat gcaagtagcc
    40921 tcatcaaggg gagaaagttc aagaagaatg gcttcatcat catctagtag cagtagtagt
    40981 aacaatggta aaaatatgag agtggtagca actgcatata ctggtcatag tataactgca
    41041 acaggaacaa aacctaagtg gggaacaata gctgtagacc ctaaggttat accatatgga
    41101 actaaagttt atatacctca atttgacatg gtgtttactg cagaagactg tggtggagca
    41161 ataaaaggaa ataaaattga tatctacatg aatgattcaa aaacggttta taactgggga
    41221 agaaaaacta tagatatacg tatattaaaa tagttaaata atttatattt ttactggaaa
    41281 ttatattttg attatataga gaagaaactc tttatagatt cttaaaatat aatttccagt
    41341 ttttattttg gatatgtaat aaaacaggta aattttaacc atgtatagat aaaagctatt
    41401 taaattataa tttactatca gataaatcta taaaaattgt tgcaaagagt caaaatatat
    41461 gatattatag atttcgttga gaaggatttc attatgggag tggatgggtg ccggtgtgcc
    41521 ctctagtctt caaaactagt atgaggggtt aagagcctct taggtgggtt cgattcccac
    41581 gcattcccgc caatatgcaa aaggtatctt ttaattaaaa gatacctttt taaatttatt
    41641 ttaagataat ttatttttaa taatatataa tgttttgtat tatataatgt aaattttcta
    41701 atactattat atatttagat aaaaatctag gagtgtggaa agatggaaaa taattttaca
    41761 acgagaacta gttttctagt tggagatgat gggatagaaa aacttaataa ttcaaatata
    41821 atagtatttg gagttggagg agttggtagt tttactgttg aagctttagc aagagctgga
    41881 gtaggcaata ttactattgt agattttgat gatgtagata taaccaatat aaatagacaa
    41941 ataccagccc tacattcaac tgtaggaagg tacaaagttg atgtcatgga agaaagaata
    42001 ttagatataa atcctaatat caatataaaa aaaataagaa gtctttataa taaagatact
    42061 agtgacgaaa tactaactga aagatatgat tatgttgtgg atgcgataga catggtaagt
    42121 tccaaaatac atctaattga aacttgtgaa aaaaaagggt tgaaaataat aagttctatg
    42181 ggtatgggaa ataaattaga cccaactaaa atagtcgtta cagatataca caaaacaagt
    42241 acttgtcctc tagctaaagt tatgaggaaa gaacttaggg atagaggtat caaaaagtta
    42301 aaagtagtat attctactga acaaccaata gaacttaaga agaaagtaat gaatggtaga
    42361 aaagtaacac ctggtagtgt atcatttgtc ccatctgtag gaggtttaat aatcgcatct
    42421 gtaatagtaa atgaactatt aggacaataa gttttaaata ttttaattta taaattttat
    42481 atacaaattt gaaggctaga agcatgaata taaagcaatt ttatattcat acttttagcc
    42541 taattatttt atattacctt attttatgta gttgtagttt tattttttag attatgtatc
    42601 tatttcataa ttatattttg taaatataat ttctccatca ct
//
LOCUS       DACTGW010000007        41182 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.007, whole genome shotgun sequence.
ACCESSION   DACTGW010000007 DACTGW010000000
VERSION     DACTGW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41182
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.007"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(21..683)
                     /locus_tag="I9573_000307"
     CDS             complement(21..683)
                     /locus_tag="I9573_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HAT4794951.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHAYSTTIQQNKDTLSQIVVF
                     PTGNYNKNEANAMVNRLANIDGKYLNVLKQDNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAEAFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(783..868)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1054..3909)
                     /locus_tag="I9573_000308"
                     /pseudo
     CDS             complement(1054..3909)
                     /locus_tag="I9573_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
     gene            3975..4051
                     /locus_tag="I9573_000309"
     tRNA            3975..4051
                     /locus_tag="I9573_000309"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4009..4011,aa:Arg,seq:cct)
     gene            complement(4260..5705)
                     /locus_tag="I9573_000310"
     CDS             complement(4260..5705)
                     /locus_tag="I9573_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAT4794952.1"
                     /translation="MASIKQRKSSFSVIYWYLDSAGERKQKWDTLETRKEAKQRKAFI
                     EYYQEKFGYVIVPLEEQFAHQIENSKKELDVVDKDITLRDFLEIFVNLYGTSKWSPST
                     FSSKVGSIENYINPIIGDWKLNEITTKSLSKYYNDLLSVPEVPRANRKATGRCVQPAN
                     IKKIHDIIRCALNQAIRWEYIDTNKRNPASLATLPKIPKTRRKVWSVQTFREAVKATE
                     DDLLSICMHLAFSCSMRIGEITGLTWEDVIIDEESIATNNAKVIINKELSRVNAEAMQ
                     KLKEKDILKIFPTQKPHCTTRLVLKTPKTETSNRVVWLPKTVAELLVQYKKDQQELKE
                     FLGSAYNDYNLVIALDNGNPVESRIVRDRFQKLCEENDYEVVVFHSLRHLSTGYKLKM
                     TNGDVKSVQGDTGHAEAEMVTDVYSEIIDEDRRFNAQKMDKEFYSTLNDDTDNPKQDT
                     DLTDSDMALLELIKSLSPEMKEQFLKQALQR"
     gene            complement(5724..5981)
                     /locus_tag="I9573_000311"
     CDS             complement(5724..5981)
                     /locus_tag="I9573_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4794953.1"
                     /translation="MTLLDECILLLSQMKGVLIVISDTQNAQSPTKEKKTYTVEEIAE
                     QLDISKKVAYSLVKSGQFSYVRAGKAIRVSKVSFDKWLNEI"
     gene            complement(6036..6125)
                     /locus_tag="I9573_000312"
                     /pseudo
     CDS             complement(6036..6125)
                     /locus_tag="I9573_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843376.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6111..6347
                     /locus_tag="I9573_000313"
                     /pseudo
     CDS             6111..6347
                     /locus_tag="I9573_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6233..6475
                     /locus_tag="I9573_000314"
     CDS             6233..6475
                     /locus_tag="I9573_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794954.1"
                     /translation="MARYLTNVSVCFIQLSKSCGESVLLSYKSRGRAGKRGLNKSCSA
                     VTACYSSSISKAISNPKIILISASLISPFNSISTTI"
     gene            complement(6376..6624)
                     /locus_tag="I9573_000315"
     CDS             complement(6376..6624)
                     /locus_tag="I9573_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4794955.1"
                     /translation="MKHTMKKPSYYLLSQAMDGDEKAIEKILAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            complement(6631..7047)
                     /locus_tag="I9573_000316"
     CDS             complement(6631..7047)
                     /locus_tag="I9573_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAT4794956.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQKKLKRRQKGETLFC
                     ELPEIVVENLAVWDDYDTDYTIFNVCGSDIRICDDELAEALKHLSERNRENLLMYYFL
                     EMSDTEIAKRQNISRSGVFQNRHNSLELMKKILKEK"
     gene            complement(7495..8331)
                     /locus_tag="I9573_000317"
     CDS             complement(7495..8331)
                     /locus_tag="I9573_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAT4794957.1"
                     /translation="MEKEELKILEELRRILNSKNEAIVILNNYFKGGVGKSKLSTMFA
                     YLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKNGNLASSIVHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSTTIKSN
                     LEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMLAYS
                     MQ"
     gene            complement(8476..8760)
                     /locus_tag="I9573_000318"
     CDS             complement(8476..8760)
                     /locus_tag="I9573_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HAT4794958.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPECTFTLAEHFR
                     KKKLTKTNVKELLEGKETLVKGIKTKDRKSYNAVVKIGEKGYIDFISFSK"
     gene            complement(8906..9643)
                     /gene="erm(B)"
                     /locus_tag="I9573_000319"
     CDS             complement(8906..9643)
                     /gene="erm(B)"
                     /locus_tag="I9573_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAT4794959.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(9768..10109)
                     /locus_tag="I9573_000320"
                     /pseudo
     CDS             complement(9768..10109)
                     /locus_tag="I9573_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(10161..10319)
                     /locus_tag="I9573_000321"
     CDS             complement(10161..10319)
                     /locus_tag="I9573_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta-toxin"
                     /protein_id="HAT4794960.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGS
                     GKTSLRRR"
     gene            complement(10321..10593)
                     /locus_tag="I9573_000322"
     CDS             complement(10321..10593)
                     /locus_tag="I9573_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAT4794961.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(10610..10825)
                     /locus_tag="I9573_000323"
     CDS             complement(10610..10825)
                     /locus_tag="I9573_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAT4794962.1"
                     /translation="MVVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            11012..11371
                     /locus_tag="I9573_000324"
     CDS             11012..11371
                     /locus_tag="I9573_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4794963.1"
                     /translation="MRKAKETQTFDFRPLGLAIREARERAGLSRNDLGDKVFYGERHI
                     ADIENVGSHPSFQLFHDLVTMFNISVDEYFYPAENVVKSTARRQIETSLDLLSDNELK
                     IIQGTIDGILNSRESKK"
     gene            complement(11479..11667)
                     /locus_tag="I9573_000325"
     CDS             complement(11479..11667)
                     /locus_tag="I9573_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT4794964.1"
                     /translation="MDKQLWVLCPICNNKTRTKVREDTELINFPLFCPKCKTETLINI
                     KHGKVTIIKEPDAKTQSR"
     gene            complement(11872..12063)
                     /locus_tag="I9573_000326"
     CDS             complement(11872..12063)
                     /locus_tag="I9573_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HAT4794965.1"
                     /translation="MRKCLRCHEEMVENLDVKVDMQGYGIRITTKGVFGTTVEKPKVA
                     VCPKCGEVSLYIENFKSIK"
     gene            complement(12076..12720)
                     /locus_tag="I9573_000327"
     CDS             complement(12076..12720)
                     /locus_tag="I9573_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAT4794966.1"
                     /translation="MKGLLMKDLLVIQKKYGITRIIMDIFIIVALMLFLKGTGAIYIS
                     LLLIPVEIMTMIISLTTCDEQWKWGKYVVALPVSKTTIVKSRYIVALIMTCIGFGVAL
                     LVNTITYFIFPQFAFGYYMFIACASFAVTLVFLSFVLPSNYSLGVNAGFAVMFTLVII
                     LVVLAVWTNVTNNSILWFVVDNFELSIGIAFIIVIALAFSSYLLSKAFFTRKYN"
     gene            complement(12717..13580)
                     /locus_tag="I9573_000328"
     CDS             complement(12717..13580)
                     /locus_tag="I9573_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4794967.1"
                     /translation="MAENILEVKNLTKDYGDFVLNDLSFSIPRGVIMGLIGENGAGKS
                     TTVNCILNEIKKNSGNILIFGKDHIIDECEIKDKIGIVFDENHFPDIFTPKEIEKYMS
                     GIYTNWDSIAFRNYLKQFELPAKKKIKDFSKGMKVKLAFAVALSHKAELLILDEATSG
                     LDPIIRDDILDILIDFVQDENYSILFSSHITSDLEKIADYITFIHKGRLVFCSLKDDL
                     IDNYGVVSCGASVFDTLDKTEIISYQKEDYQYKVLVRDRNKIAKDFPKALVEPATIEN
                     IMLFYVKGELQ"
     gene            complement(13581..13958)
                     /locus_tag="I9573_000329"
     CDS             complement(13581..13958)
                     /locus_tag="I9573_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4794968.1"
                     /translation="MEIFINNHASIPIYEQISSQIKALIINGELKAGEALPSIRGLAK
                     SLHISVLTVQKSYDVLQKDGFIETTAGKGCFVSAQNQDFYIEEQQKKIEDRFVEAIEI
                     ARASGINLDKLIGLLKLLYEEDE"
     gene            complement(14079..14990)
                     /locus_tag="I9573_000330"
     CDS             complement(14079..14990)
                     /locus_tag="I9573_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT4794969.1"
                     /translation="MFKKNKKQTETIKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWNNSKEAIEARTQA
                     ISGYLTKELQDLNIDTIRTDIPTSSTVTDVIVWHIEQSGTDTFSATYEVDQQIKEGEQ
                     TSNVKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYDPKTPETDASVDADTANDATA
                     FLETFFKLYPTATENELAYYVSGNVLEPIGRDYLYSELVNPIFTKDGENVKVKVAVKF
                     LDNQTKATQVSQYELVLHKDSNWKIVG"
     gene            complement(15006..16013)
                     /locus_tag="I9573_000331"
     CDS             complement(15006..16013)
                     /locus_tag="I9573_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAT4794970.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGMDDLNVAIQSYNYGGGYVGYVAGKGNKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYSYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGFADLSSSYWQQHLIGAGRVK
                     Q"
     gene            complement(16010..18205)
                     /locus_tag="I9573_000332"
     CDS             complement(16010..18205)
                     /locus_tag="I9573_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4794971.1"
                     /translation="MKERIKGAFTKKKIFHFLKMVLFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTIAGVTQNGFSSTGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSKGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYLAHRARRMERRLAGAVTAGGVAGAVAGSTISNRKATRNTATKDSRGNTSSSMGQR
                     VGSKVGAVLDTKNKVKDKASAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGMVQE
                     QQSRQSGRMEKREQHRQNIADKRMELQKAQEARQAQRKADGSATTGATRPHERPATAS
                     TIPKPSAEKTQEVKRPATATTSKTNEPVKTTVIKERPLSSGTSDRKTSQPTQPVHRQN
                     VEKVVSQETRQNDTKDRRTKVQQTQSVQKNQQTTEKTRNLVMKKGQKKK"
     gene            complement(18205..20655)
                     /locus_tag="I9573_000333"
     CDS             complement(18205..20655)
                     /locus_tag="I9573_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT4794972.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIHSMQEQSKKLVTGKLKEVACQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRNGIAYEDYEYQLPKKKLNKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVVRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGIKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKGNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSNSRGLLHVIDELRREDTPISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(20633..21031)
                     /locus_tag="I9573_000334"
     CDS             complement(20633..21031)
                     /locus_tag="I9573_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT4794973.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            complement(21149..21652)
                     /locus_tag="I9573_000335"
     CDS             complement(21149..21652)
                     /locus_tag="I9573_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAT4794974.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GICEIPY"
     gene            complement(21670..22173)
                     /locus_tag="I9573_000336"
     CDS             complement(21670..22173)
                     /locus_tag="I9573_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAT4794975.1"
                     /translation="MQETRVLVETRETTGEETTSYWFDLPIDVAEFEEKLGVGAESTA
                     YRIIEKVLPYADEVHEHTSVYQLNELEFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDSTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            complement(22092..22388)
                     /locus_tag="I9573_000337"
     CDS             complement(22092..22388)
                     /locus_tag="I9573_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAT4794976.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWREMKRLSPADYEERQL
                     EDFSKFVFGMPYQTLKDVMKQQKGREEQCRKQGCWWKREKQLAKKQHHTGSTCR"
     gene            complement(22473..23249)
                     /locus_tag="I9573_000338"
     CDS             complement(22473..23249)
                     /locus_tag="I9573_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4794977.1"
                     /translation="MSLGEKIFKLRKEKGISQEALAEQIGTTRQAISKWENNQGFPET
                     EKLLQLSNIFEVSTDYLLKDEKNVKNSEERGYYVSKEMSVGFIANQKKLGLYLALGVM
                     LCIFAGIPYTLFLDNMTWRYLGMAFCFVAGIIFFVVAIFYEKQEYNILRQEPLLLDYE
                     YLKELKSEYTSKKKKYVIVAVPCTVLFIAGLIFFLFTVKGYISWTEYHSFAFFGLAVG
                     MLGFVYTINVMETYELLVKNEQYSTSFFFKLKRKIKKRLG"
     gene            complement(23453..23674)
                     /locus_tag="I9573_000339"
     CDS             complement(23453..23674)
                     /locus_tag="I9573_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794978.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(23675..23809)
                     /locus_tag="I9573_000340"
     CDS             complement(23675..23809)
                     /locus_tag="I9573_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAT4794979.1"
                     /translation="MWVLLKDFLLVSMGMGIGIVLMCILNVGKEADYKMKQLEESEDN
                     "
     gene            complement(23822..25018)
                     /locus_tag="I9573_000341"
     CDS             complement(23822..25018)
                     /locus_tag="I9573_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT4794980.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHSEDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVEI"
     gene            complement(25202..26596)
                     /locus_tag="I9573_000342"
     CDS             complement(25202..26596)
                     /locus_tag="I9573_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT4794981.1"
                     /translation="MRKIWNRGHRIRASDKHLVYHFSIGTLLFLFVAVLLLLNIKQLM
                     RTDWEHFSLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLKLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMENVY
                     HTKEKMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAQARQ
                     DGTATCEAKGDGTD"
     gene            complement(26697..27023)
                     /locus_tag="I9573_000343"
     CDS             complement(26697..27023)
                     /locus_tag="I9573_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4794982.1"
                     /translation="MNLNIVLAVICGAVALVGAFCVVFQIYHMTVIDATARGLKHPKF
                     WGVFTMSGNNSSGLLMYLIGRRKYPIVNMSESNSKELEKRKKSAGIGLLFLAIGVIGI
                     ICATLI"
     gene            complement(27039..27446)
                     /locus_tag="I9573_000344"
     CDS             complement(27039..27446)
                     /locus_tag="I9573_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4794983.1"
                     /translation="MEIGKKLKNARVQSGMTQENVAEKINVSRQTISNWENEKSYPDI
                     INVIELSNLYSISLDELLKGDEKMIEHLEESTNVVKSNQKMIWAIIVNIIVVALLIAL
                     NMFIPDNRYFLVGIFCLMVITSSALLYQIIKKF"
     gene            complement(27614..27997)
                     /locus_tag="I9573_000345"
     CDS             complement(27614..27997)
                     /locus_tag="I9573_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAT4794984.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFMQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTFGGADVDWYVKAED
                     IVLKNKGNQNAGNPQNHVPQGQTKK"
     gene            complement(28013..28339)
                     /locus_tag="I9573_000346"
     CDS             complement(28013..28339)
                     /locus_tag="I9573_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAT4794985.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKKFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            complement(28330..28559)
                     /locus_tag="I9573_000347"
                     /pseudo
     CDS             complement(28330..28559)
                     /locus_tag="I9573_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843347.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28572..31616)
                     /locus_tag="I9573_000348"
     CDS             complement(28572..31616)
                     /locus_tag="I9573_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HAT4794986.1"
                     /translation="MKKTLKRLCTGFLALATIVTALPTTPVHAESKQYWTESAERVGI
                     IEKVMNDGSIGSTFNEGMMKVEGKTAYCIDINTDFRNGYKTRADASSRMNANQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFSGARTFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLKKTSSNASITDGNG
                     NYSIAGATYGVFTDKDCTKQLATLTTDENGNTDVAEVKAGTVYIKELSAPAGYKVDKT
                     VYSLKIEAGKTATLNVSDTPKVTDTLIELFKIDMETKKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSYGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTIEETKAPNGYLLEGAYMQAGDKSEQIKGLYVTQITEDDDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDMKPQGSATLKDTAFDIISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFTITENGKIVDLTDKAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHIVVTDNNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYVIEELRSESNKGFELIPPFEIVVSRN
                     NLLIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEITIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRAENDYTFVADEEEMKVEIAYTFNASALGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATNKDGNKELEAGKNVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKCVENDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKPEEPEKPETPQIPEKPS
                     RPSDSPKTGDSTNVMAFLVMLLASAGGLAGTYLYKRRKMKKS"
     regulatory      complement(32135..32220)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(32346..33524)
                     /locus_tag="I9573_000349"
     CDS             complement(32346..33524)
                     /locus_tag="I9573_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAT4794987.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMFTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIVSIMIKIWLSRFNKFVGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKDMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            33896..34081
                     /locus_tag="I9573_000350"
     CDS             33896..34081
                     /locus_tag="I9573_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAT4794988.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEVYKILQKLDIDFERVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(34146..36920)
                     /locus_tag="I9573_000351"
     CDS             complement(34146..36920)
                     /locus_tag="I9573_000351"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HAT4794989.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAQALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRSMNSNSKN"
     gene            complement(37092..38348)
                     /locus_tag="I9573_000352"
     CDS             complement(37092..38348)
                     /locus_tag="I9573_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAT4794990.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(38420..39970)
                     /locus_tag="I9573_000353"
     CDS             complement(38420..39970)
                     /locus_tag="I9573_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HAT4794991.1"
                     /translation="MRKINSQEKNKDSGLLMRALNVVERAGNKLPDPVTIFLLLCIIV
                     VILSAVISNLGVEEIHPSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTM
                     LGAGVAEKSGFMEVLMKKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVF
                     LGIGRHPLVGMFAAFAGVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNL
                     YFAFISAIVLTFAGAFVTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLV
                     VVVVIVALCIGDNAFMKDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIK
                     SDKDLVSMMGSSMSDMGGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSY
                     GLIIGFILLSGFINLFVGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNP
                     ISPLFPYFPVILAFARRYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGP
                     GGGISYVL"
     gene            complement(40013..>41182)
                     /locus_tag="I9573_000354"
     CDS             complement(40013..>41182)
                     /locus_tag="I9573_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4794992.1"
                     /translation="SEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLVSLDIEIL
                     DLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIEYKSRYDGIMHACGHDIHTS
                     CLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAIFGLHNHP
                     DIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMGIQTIVSR
                     NISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLENIVCEIAR
                     GYQATAKLDYLFDLPAVINSKEMYTIACKSVCDLYSEDAIVDPIPSTGGEDFSIFMEK
                     APGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKLKNKI"
BASE COUNT        11706 a         8539 c         6378 g        14559 t
ORIGIN      
        1 attttaaatt tattaaagct ctatttagct aggttttgca aaaagctata agtttgtgga
       61 caagctgatt ttaacttact atttgtatct tgatttaaat agtaatatgc aaatgcttct
      121 gcaaaaaatt cttctggata tacacctaag tagtttacat tacctaaaga acgaccttct
      181 ttagcaaata tttgtttgaa ttgtgctgat ttagatatat catttaatac tatatggtct
      241 attgcatgtg ctgtttcatg taattctaaa tttattgcat catgcccttt acctttgtta
      301 ctaaatccta ttctaagagc tactgtactt ccacctaagc ctggaacatc atcccaagtt
      361 tttccagtac cttcccatcc cttaggaact acacctttta aataagcata ttctttttca
      421 tctgttaact ttccacttaa taattttatt tttaagttat cttgctttaa tacatttaaa
      481 tactttccat ctatattagc taatctattt accatagcat tagcttcgtt tttattgtaa
      541 tttccagttg gaaaaactac tatttgacta agtgtgtctt tattttgttg tatagtagta
      601 ctatatgcat gtgcacttac tggaactgaa cttattaaga atattgatat tatagctatt
      661 aataattttt tacttggtct catattattc cctcctttga gttaggccgg gagtaataaa
      721 tttcaaaata aaaaagatac ccaagagtat ctttgtatat acaaaaagaa atttttactc
      781 ttttcggcaa ctggctggca tggtttacta aactttagcc cctatagctt tgcgtgacta
      841 ggtttcccta gttttgcctc atttaatttt atatctatat tatagcttat atctttttaa
      901 ttgtctatat ccctttggaa cttttttcta aaaagttatt atttttttac ataaatcttc
      961 aaaaaaaaat atcttctaac aatattttca ttattaaata ttgttttaga agatattttt
     1021 taatataact tatatttttt ataatgttag ggtctaattt gtatttttat ttcttcttaa
     1081 tacgacaagt cctacaatag ctcctataac taaaagtatt tctccaataa ttgtagtact
     1141 atcccctgtt tttggtggta ctggtggatt taccaaagta tcatctttag ctttattatt
     1201 tttttcttct gacactgatg gctttaccac ttctggtatt ggaacaatct gtttaacagg
     1261 agtttctatc acctcttctg atggtggtac tggtggattc aatatctctg gtattggagg
     1321 atttattata tcttctgacg gtggtactgg tggatttata actatagtat cctctgtatc
     1381 agatggattt gatggtttcc ttggtttatc tgtgcttggt ggcactggtg gatttactat
     1441 tggttcatct gtatttggcg gtacaggtgg atttactggt ggattttctt gtcttatgtt
     1501 ttctatattt ccgatatcta ctattctacc aatatcttta ttaccaattt caaccttaat
     1561 tttatccttc agtttttcat atccacttgg agccatcact tctactaaat aatatattcc
     1621 tggttttttt acactagatt gaattacacc atcttcatca ctaactaaat ccttaactac
     1681 tatatttcca aatgcatctt ctaaattgaa cttagcacct gaaagtacat ttttatctaa
     1741 agcatccact tttttagctt caaaagcata accttgctta gtagtctcta ctcccggagg
     1801 acttgaacca ttaatctgag ccacattttg tatagtctcc ccaactttta catttgtgaa
     1861 gtctgcatca aatgtaaccc agctttgcac aggttcccct tcttttatct gtttaaattc
     1921 gtatttattt actaatctta ttgtattttt tccatctcca gcttcttttt taagttcaag
     1981 attatcagaa tattttatat tagatatttt tagagatggg tttacttcat ccaaaagatt
     2041 tacttgtcct gcatcataaa ccccataagt tgacatattg attttgtatt taacaatctg
     2101 gttatcacta ctatttaaaa cctttttatc aacttctttt tttgcgctta ttaatccaga
     2161 atttttctta aagtttatct catcattttt atcaatttca ctattatttg catcaaggac
     2221 agctctattt atataactat cttcaatact aattaccttt aatgtataag ctataaaaaa
     2281 ctttgattct acttcttctt tactgagatt aattgtaaat ccagttataa ctttatctac
     2341 ttgcccactt tcatttcttt tttcttctac tttatatgaa atattattgg caattttttc
     2401 tttactccaa ggagtccagc tcccattcat tggaacttct acaactctaa aatcatgccc
     2461 atcctctcct gaagtagtca atctcatacc ttctggtata ttatctgtta taacaacatt
     2521 acttattggt gatttatcat catttattga aatctgatag tttattactt gaccaacttg
     2581 agtaatattt gttgctcctg aatgtccttt agtaagaaga taattatctc ctctatcaaa
     2641 tatacttaca gtagcatcat cttcttgtat cttcttacca tcagattcaa tcctagctac
     2701 attcttatat ttattaccat gttttgcatt tttgtcactg attctagcct tatacttaac
     2761 agtatacgat ttatctatat ttccaaaatt aactgaaata gaatttttac tctgttcaat
     2821 attaaaatca ctaacttctt ttccatcact tgttactaca cttacagaac catttatcaa
     2881 tgtcaatcct tcaggaagct catcatacaa aataatatct tttaactctt cctgcttttc
     2941 atttacttgt atactgtaat ttatatcttt atttttaact ggcataaata cagtagttga
     3001 ttttcttcct tcaacaagct ttttaagtgt tcctttagca tctggaacat ccgttttatc
     3061 tccaggtttt atttctacat ctatatctcc acttccactt tctatttttc cagtggtttc
     3121 aatatgtatt tttgcattag tatcttcatc aaccttttta acatatgctt ccaaacctat
     3181 tttaccttgc attacacttt ggccattagg taattctcta aatgtaagtg tatactctcc
     3241 attactttct gttttatcaa agcattcact tgtataatct aaattaactt tattgaatac
     3301 atctggcacc ttaaaagtta ttgaatctcc tgcttgtatt ccttttgatg ttggagtata
     3361 agttaaatta aatgatattt ttttcattga ttccgtttct attgtattgt tattaatttt
     3421 tacaacaaat ccatagtctt cattaagttc ttcttctaaa actttcaata aattttcatc
     3481 ttctaataga ttatcttcct ctggagtttt ttctgtattt ttattgtcta gtggtttttc
     3541 atcttctaaa gcttgttctg actttttatt atctgttgac ttatcttctt ctggtatttg
     3601 ctctggcttt ttattatcta ttgacttttc tccttctgga gtttgctctg gctttttatt
     3661 atctattggc ttttctcctt ctggagtttg ctctggcttt ttattatctg ttgacttatc
     3721 ttcttctgaa gtctgttctg gttttttatt atctgttgaa ttatcttcct ctggggtctg
     3781 ttctggtttt ttattatctg ttgccttatc tttctctgga gtctgttctg gttttttatt
     3841 gtcgctttcc ttctcctctc cagttgatga attagttgaa ccactttgaa tctgactatt
     3901 ctctcctaag ggctaggtgg aacaaacgcc ttttgatatg ataactagat aaaattgaat
     3961 agatttttaa gaatgtccca gtacctcagc aggatagagg gttcgcctcc taagcgaaac
     4021 gtcgcaggtt cgactcctgt ctgggacgcc acaaactccg ctgaaagcct tattttacaa
     4081 ggttttcagc gtttctttat ttatggcttt ctatttacgc tgaatacgaa ttgttttcaa
     4141 tctgtcattt tttattcgat atttttttaa gacttttaag gaaataggct tgctaacact
     4201 tttttatttt tgaagaaaaa tcgctggttt ttttggaatt tgctaacact tttgaaattc
     4261 tatctttgaa gtgcctgctt taaaaattgc tctttcattt ctggcgataa agatttaatc
     4321 agttccaata acgccatatc actatcggtt aagtcggtat cttgctttgg attgtctgta
     4381 tcatcattaa gcgtagaata aaattcttta tccatttttt gtgcattgaa acgtctgtcc
     4441 tcgtcaatga tttctgaata tacatctgta accatttctg cttctgcgtg accagtatcg
     4501 ccttgtacgg acttaacatc tccgtttgtc attttgagtt tataacccgt acttaaatgg
     4561 cgtaggctgt gaaataccac aacttcataa tcgttttctt cacagagctt ttgaaaccta
     4621 tctctaacaa tacggctttc cacgggattg ccattatcca acgcaataac aaggttgtaa
     4681 tcattgtagg cacttcctaa aaattccttt aattcctgct ggtctttttt atattgcacc
     4741 aatagttccg caactgtttt aggtaaccag acaacacgat tacttgtttc tgtttttggc
     4801 gttttcaaga caagccttgt ggtacaatgc ggtttttgcg ttgggaaaat ctttaaaata
     4861 tccttttcct ttaatttctg catagcttct gcatttactc ttgaaagttc tttattgatg
     4921 attacctttg cattatttgt agcaatggac tcttcatcaa taataacatc ttcccatgta
     4981 agtcctgtaa tttctccaat tcgcatagag caggaaaaag ctaagtgcat acaaattgaa
     5041 agcaaatcat cttccgtggc ttttaccgct tctcgaaatg tttgaacact ccacactttt
     5101 cgcctagtct ttggaatttt tggtaacgta gccaaagacg cagggtttct tttgtttgta
     5161 tcaatatact cccagcggat agcttgattt aaggcacagc ggataatatc atgtattttt
     5221 ttgatgttcg ctggttgaac acaacgacca gttgcttttc tgtttgcccg tggtacttct
     5281 ggaacgctca ataaatcatt gtagtatttt gatagacttt ttgtagttat ttcattcagt
     5341 ttccaatctc caataatcgg attgatataa ttttcaatac tgcctacctt actactaaat
     5401 gtactcggcg accatttaga tgtgccatat aaattaacaa aaatttctag gaaatcacgc
     5461 aatgtaatat ctttgtctac aacatctaac tcctttttag aattttcaat ctgatgtgca
     5521 aattgttcct ctaatggaac tatcacatat ccaaatttct cttgatagta ttctataaag
     5581 gcttttcgct gttttgcttc ttttcttgtt tccagcgtgt cccatttttg ttttctttct
     5641 cctgcactat ctaagtacca ataaataact gaaaaacttg attttctttg tttgattgaa
     5701 gccataattt tctctcctta tagttaaatt tcatttaacc atttatcgaa agaaaccttt
     5761 gatacccgta tggctttacc agcacgaaca taactgaatt gaccgctttt tactaatgaa
     5821 taagctacct ttttgctaat gtccagttgt tcagcaattt cctctactgt ataagtcttt
     5881 ttttcctttg taggagattg tgcattttgt gtatcactta tcactattaa gacccctttc
     5941 atttgtgaca gcaacaaaat acactcgtct aataaggtca ttataacaca cctaaataaa
     6001 aagagtagat agattttgtt gctgaaacta tcaattataa gcaggcagac ggctttggaa
     6061 agctccgtta gtatctcaac tattcccatt cttgtgggca gaaccgcacc atgcggacgt
     6121 atcattattc tgtgaggata ggtcatggca aaacgacttt tccaacagtc gctctcggat
     6181 cactgcgtgg gtcgctcgct ttcttttgaa aaggtcgtgg cgtacagttc ccgtggctcg
     6241 ctatctcaca aacgtatctg tctgctttat tcagttgtca aagagctgtg gcgaaagcgt
     6301 gttgctttca tataaaagca ggggcagagc aggaaagagg ggattgaaca aatcatgctc
     6361 tgcggttact gcttgttatt cttcttcgat ttctaaggca atatcaaacc ctaaaatcat
     6421 tttaataagt gcttctctga taagtccctt taattccata tctacaacaa tatagacatt
     6481 gccgtattca tcatagagtg gtcgtagaca gcactttgat atgtaagggt cataaaatgc
     6541 cagtatcttt tcaatcgctt tttcgtcccc gtccattgcc tgcgaaagca agtagtagga
     6601 cggcttcttc attgtgtgtt tcatttgcta tcatttctcc tttagtattt ttttcataag
     6661 ctctagtgaa ttgtgtcggt tttgaaaaac accacttctt gaaatatttt gtctttttgc
     6721 aatctcggta tcgctcattt ccaagaaata atacatcaac aaattttctc gattacgctc
     6781 agacaaatgt ttcaaggctt ctgccagttc atcatcacaa atacgaatat cactaccaca
     6841 tacattgaaa atcgtatagt ccgtatcata gtcgtcccag acagccagat tttctacaac
     6901 gatttctgga agttcacaaa aaagcgtttc tcccttttgt cgtcgcttta atttcttctg
     6961 atagtcctta acaacatgtc ggacaacttt cttcaaacaa ctttcaaaac ggcattgaac
     7021 tgttttctgg aagtcagatg gtttcatcaa tctcaccccc tttccgttac gatatgaaaa
     7081 gtggttatcc ctctttccgc accatatagg gaacgacagg tgtgatttgc taagttgaaa
     7141 ccgaaaaaag ttgaaaaaaa tttttaacaa aacaaaaaca gcccgataat gtgtaattac
     7201 acaaacagac tgttttataa attctattga tatgaatttt taattcttgt aaagggtgta
     7261 atgattgtaa tagtggactg aaatatttat tcagttgaga acggcagaac gttcttccgt
     7321 atgcaatcat ggtatagccc cctttaacca gagctatgcc ttatttactc ctaagaaaaa
     7381 aggacagttc tggttatatc caaaaccgtc ccgttatttt tcaagacgca caaaagtgta
     7441 ttatctagga acggtttttt ttatttaccg cacacgataa cagcttttga attattactg
     7501 catactatat gcaagcattt caaaaaatac gttctcatac atagataaaa cttttttgtc
     7561 atagcctttg tgttctgtaa tgccgttttt agaccaggta cttactttat tacttcgctt
     7621 gataatattt tggaaaacca agttatcttc tttatgttgc ttgtacagtt cttccaggtt
     7681 tgattttatc gttgtgctgt ccgtatcaac taaataagga acaaaaccga tcatatctag
     7741 tccagggtta aactgttctt gtaaatcaat caaataggaa atatagtttt gaatgttgtt
     7801 tgtactttct tcttctgctt gtaaagggat catgacgtaa tcacttgcca cgattgcatt
     7861 atttgtataa acgcttggcg ttggtacagt atcaataata atgagatcat agtcactttt
     7921 taaaggtgct aaaagagtag caagcaatct actttcattt tcaaaagtcc atgagcgagt
     7981 taattttggc agtaacatca aatcaaacgt gccagggatc aagtctaaat tatcagtcaa
     8041 atgaacaata gaagaagcca agtttccatt tttcagtcct tcataaaaat taacacgtgg
     8101 caattctacc ttaaatgttt ttgctaagtc ttttgtcaat gttgcttgta agtccttatc
     8161 gatcattaaa acttttaaat taaatttgtc tgtcaagtaa gcaaacatag tcgataattt
     8221 tgactttcca acaccacctt taaaataatt gtttaagata acaatcgctt catttttact
     8281 gtttaaaata cgtcttaatt cttcaagtat tttgagttct tccttctcca taactacgcc
     8341 ccattctttt tttgtatagt aatattgtat cattaggaat cacaaatcac aagtgatttg
     8401 tgattaatca cttgtgattt gtgataagtg atttgtgatt aatatataaa agccctcttt
     8461 aaagggcttt tatgcttatt ttgaaaaaga gataaaatca atatatccct tttctccgat
     8521 ttttacaacg gcattgtagg actttctatc tttcgttttg attcctttta ccagggtttc
     8581 ttttccctct agtaattctt ttacatttgt tttggtgagt tttttctttc taaaatgttc
     8641 agctaaagtg aaggtacatt caggataatt tgaacaacca taaaacgatt tttttaatac
     8701 aatattgttg ccacacttag gacattttcc tacaaggggt cccgagcgct tagtgggaat
     8761 ttgtacccct tatcgataca aattccccgt aggcgctagg gacctcttta gctccttgga
     8821 agctgtcagt agtataccta ataatttatc tacattccct ttagtaacgt gtaactttcc
     8881 aaatttacaa aagcgactca tagaattatt tcctcccgtt aaataataga taactattaa
     8941 aaatagacaa tacttgctca taagtaacgg tacttaaatt gtttactttg gcgtgtttca
     9001 ttgcttgatg aaactgattt ttagtaaaca gttgacgata ttctcgattg acccattttg
     9061 aaacaaagta cgtatatagc ttccaatatt tatctggaac atctgtggta tggcgggtaa
     9121 gttttattaa gacactgttt acttttggtt taggatgaaa gcattccgct ggcagcttaa
     9181 gcaattgctg aatcgagact tgagtgtgca agagcaaccc tagtgttcgg tgaatatcca
     9241 aggtacgctt gtagaatcct tcttcaacaa tcagatagat gtcagacgca tggctttcaa
     9301 aaaccacttt tttaataatt tgtgtgctta aatggtaagg aatactccca acaattttat
     9361 acctctgttt gttagggaat tgaaactgta gaatatcttg gtgaattaaa gtgacacgag
     9421 tattcagttt taatttttct gacgataagt tgaatagatg actgtctaat tcaatagacg
     9481 ttacctgttt acttatttta gccagtttcg tcgttaaatg ccctttacct gttccaattt
     9541 cgtaaacggt atcggtttct tttaaattca attgttttat tatttggttg agtacttttt
     9601 cactcgttaa aaagttttga gaatatttta tatttttgtt catgtaatca ctccttctta
     9661 attacaaatt tttagcatct aatttaactt caattcctat tatacaaaat tttaagatac
     9721 tgcactatca acacactctt aagtttgctt ctaagtctta tttccatact cgctcttaat
     9781 acaatatagt gtacctcgta atcttcttgg gcaagagctt tccatggctc caaaaaccat
     9841 ggcccgacaa tcccatctac aattacatca tatccgccac gagcatatcg ctttgcagcc
     9901 tctaaaaagg cttcaatgac aaccagattt tgcttgttgg attctggcaa atgtggtggt
     9961 attgcgcctt tgcttaagta atgaaaaaag tcatcggtgt gcatatgcac tgatttatcc
    10021 atatctgatt cctttgcgac aattgacgca gttgttgttt ttcctgtccc cggtgagcct
    10081 gtaatcacaa taattcttcc ttgattcatc tatattttcc ctcacaattt gaatttatca
    10141 ctttgttcct gcctgctcaa tcatcttctt cgcaaactcg ttttccctga ccctggttgc
    10201 ccaccaagta aaaaagcggt tggcgattca accgcttttt ttccttgaac caattcttct
    10261 aaattatcat ttaagcgatt ttcaaattgt ttgtcagtaa aattgactat atttgccata
    10321 ttaagccact ttctctttat tcaaaacttg ttttgaatag cgattcattg accgccaata
    10381 ctcatgaacg gcttgtaatt cttctaaaga ataatcaaaa agatttacac gttttgcaag
    10441 ctttaattgg tttaataaat tgacttccaa taattgagag tcatttttat ttaattcatg
    10501 attcaaaaca tagtttaata ctcgattata aacgcttgcc gataattcgt taatgatctc
    10561 tatttcaaat gttttttcat aagtaactgc catttcattt tcactccttt taaagtttgt
    10621 caggtaaata tttccgaata tattcttcta aggctttatc cataacttct tttacgtttc
    10681 cgccattctt tgctgtttcg atttttataa tatggtgcaa gtcagcacga acacgaaccg
    10741 tcttatcccc cataatatct ttttttgcac tgattggtgt atcatttcgt tttgcttttt
    10801 gtgcgcctaa atttcccaca accactcact tctttctatt tcttcttact cttattttat
    10861 catcaacaat cacaaatcac aagtgatttg tgattaatca cttgtgattt gtgattctat
    10921 tctttttcgt tttctaattg aatgattcgc tcaattttaa cacattctaa atatacagca
    10981 tgaagtataa actcatgcta ggtgatttaa aatgagaaaa gcaaaagaaa cacaaacttt
    11041 tgatttcaga cctttaggac tggcaatcag agaagcccgt gagagagcag ggctttctcg
    11101 taatgattta ggcgataaag tgttctatgg agaacgccat atcgcagata ttgaaaatgt
    11161 tggttcacac cctagttttc aattattcca tgatttagtt accatgttca atatatcggt
    11221 cgatgaatat ttctatccag cagagaacgt cgtaaaaagt acagctcgcc gtcagataga
    11281 aacctctctt gatttattga gtgataacga attaaaaatc attcaaggta cgattgacgg
    11341 tatcttgaac agcagggaga gcaagaagta atcagcttct tgttttccct ttttgctatt
    11401 ctgcattatc tatttgctga tctgctccgc aggtaacaaa taagagccaa caatctataa
    11461 gtcataataa cctacaaatt atcggctctg cgtctttgcg tctggctctt tgataatagt
    11521 tacttttcca tgctttatat tgattaaagt ttccgttttg cacttcggac agaacaaagg
    11581 aaaatttatc agttctgtat cttctcgcac tttagttcgt gtcttattat tgcaaatagg
    11641 acataatacc cataattgct tatccatagc ccccccttat tcttttaaga aatttaattt
    11701 ttgctttttc gtagtgatat aaacatattt tttcggcgta aaatgtctat ttccaaaata
    11761 ccttttgaaa tttgcttgca tctctgggta tgtactattc ataaattctt caatatccgc
    11821 tcggacgtta gcaaacaata aaccctcagt ctatatgcag attttaatca tttattttat
    11881 tgatttaaaa ttttcaatat ataaggaaac ttcgccacat tttggacaaa ctgcaacttt
    11941 aggtttttct accgtagttc caaaaacgcc tttagtagtt attctaatac catatccctg
    12001 catatcaact ttaacatcta aattttcgac catttcttca tgacatctaa gacattttct
    12061 cataacaata cctcactaat tgtattttct agtaaaaaat gcttttgata gaagataaga
    12121 actgaaagca agagctataa ctattataaa agctatgccg atactcaatt caaaattatc
    12181 gactacaaac cataaaatag aattgttagt cacatttgtc caaactgcca atacaacaag
    12241 aataatcact agagtaaaca taaccgcaaa acctgcatta actccaagtg aataatttga
    12301 aggcaataca aatgacaaaa atactaaggt tacggcaaaa gaagcacaag caataaacat
    12361 ataataacca aatgcaaatt gagggaatat aaaatatgtt atggtattaa caagcaaagc
    12421 taccccaaag ccaatacaag tcataatcaa agcaactata tatctgcttt ttacaatcgt
    12481 agtttttgaa acgggtagtg caacaacata tttcccccat ttccattgtt cgtcgcaagt
    12541 cgttaaggaa attatcattg tcattatttc aactggaatt aacaataaac taatgtaaat
    12601 agctcctgtc ccttttaaaa ataacatcaa agctactata atgaatatat ccataataat
    12661 tctagtaatt ccatattttt tctgaattac taataaatct ttcatcaata agcctttcat
    12721 tgtaattccc cctttacgta aaacaacata atattttcaa tagtagcagg ttctacaagt
    12781 gcttttggaa aatcttttgc aatcttgttt ctatcacgaa caagaacctt atattgataa
    12841 tcttcttttt gataggaaat aatttctgtc ttatctagcg tatcaaagac acttgctcca
    12901 caacttacaa caccatagtt atcaattaag tcgtctttta atgagcagaa aactaaccgt
    12961 cctttatgaa taaaagtaat ataatctgct attttttcta aatcacttgt aatatgtgat
    13021 gaaaacaaaa tagagtaatt ttcatcttgc acaaaatcaa tcagtatatc aagaatatca
    13081 tctcgaataa tagggtccaa tccacttgtt gcttcatcta atataagtaa ttctgcttta
    13141 tgtgaaagag caacagcgaa tgcaagtttt actttcattc ctttagaaaa atctttaatt
    13201 ttcttctttg ctggtaattc gaactgtttc aaataattac gaaatgctat actgtcccaa
    13261 ttggtatata ttcccgacat atatttttca atttcttttg gtgtaaaaat atctggaaag
    13321 tggttttcgt caaatacaat tccgatttta tcttttattt cacattcatc aattatgtgg
    13381 tcttttccaa aaatcaaaat gtttccactg tttttcttaa tttcgttcaa aatacaattt
    13441 actgtggtcg attttccagc tccattttcg ccaataagcc ccataattac acctctagga
    13501 atggaaaaac ttaagtcatt taatacaaaa tcgccataat cttttgtaag gtttttaact
    13561 tctaaaatat tttcagccat ttactcgtcc tcctcatata ataattttaa taatccaatc
    13621 aatttatcaa ggtttatgcc acttgcacgg gctatttcaa tagcttctac aaatctatct
    13681 tcgatttttt tctgttgttc ttctatataa aaatcttgat tttgagcaga tacaaaacac
    13741 cctttgcctg ctgttgtttc gataaaaccg tctttttgaa gcacatcata agatttttga
    13801 acagtcaata cacttatatg caatgatttt gcaagtcctc ttattgatgg tagagcttcg
    13861 ccagccttta attccccatt tataatcaat gcttttattt gcgaagaaat ttgttcataa
    13921 ataggtatgc ttgcatgatt attgataaag atttccattt aaacaccgcc ttgttctata
    13981 ctgtatagct tatacaagta cagcataata cttaaaaata cagttgtcaa tattaaaagg
    14041 cttgcagagc ataaaaatat gatacaagcc ttttacaatt atcctacaat cttccagtta
    14101 ctatccttat gtagcacaag ctcatactgc gatacttgcg ttgcctttgt ctgattatca
    14161 aggaatttta cagcaacctt aacttttaca ttctccccgt cttttgtaaa gatagggttt
    14221 accagttcag aataaaggta gtccctgccg ataggttcaa gcacattccc cgacacataa
    14281 taagcaagtt cgttttctgt ggctgttggg tacaacttaa agaatgtttc cagaaatgcg
    14341 gtagcgtcat ttgcagtatc agcgtctaca cttgcgtctg tttctggtgt ctttggatca
    14401 tagtctgatt tttcgattgc tggtgcaaga gtagggttct gaacgattac catatccccg
    14461 tcagcgtcta catgaacttt tacagtatag gttgctttca cattgcttgt ctgttcgccc
    14521 tcttttatct gctgatctac ttcgtaggta gcagaaaaag tatccgttcc cgattgctcg
    14581 atatgccata caatcacatc tgtaactgtg gagctggtcg gtatgtcggt tctaatggta
    14641 tctatattca agtcctgcaa ttcctttgtc agataaccgc tgattgcctg cgtccttgct
    14701 tcaatcgctt ctttgctgtt attccatgtg taatatgact tcgcaaagtt cttcacgaaa
    14761 ttttctatcc cgttggtgtc ctgcaagcga agctcaatga tttctttttc atgggtggtg
    14821 tgctggtcga tagcggtaaa attcttatac accccgaagc tcacgcttgc gataagcacc
    14881 acccacaagg caatcacagt tttcttatgt gtgcctacct tgacagtacg cacctttttt
    14941 tcttttggtt ctttgatagt ttctgtctgt ttcttatttt tcttaaacat atcttcaagt
    15001 cctttctatt gttttactcg tcctgcaccg attaagtgct gttgccagta actactgctt
    15061 aggtctgcaa atcctatcgg gtcgcctgca tggtacatct ggttatttcc tacataaatg
    15121 ccgacgtggg ttacatacga accagcgtta taggtagaat ggaaaaatac caagtcccca
    15181 gcctttgcct gcgagagtgg gagatgttgg gtagcatcat actgtgcttg tgccgttctc
    15241 ggtaaggaga taccagcttt tccataacac cattgtgtca gtcccgaaca gtcaaaggaa
    15301 gtgttgggat tactaccacc atagacgtac ttccagcctt gatacttcaa cgcttcattc
    15361 attacctttt gtgccagctc tcccgacacc tgcggaacag ttaaatactg attgactaat
    15421 tccacataga acatatttcc atagctatac cgccagcctc cattcttcgc aacagctatt
    15481 ggattggtgt atgttacttt ctttccaccc gatttttcac gggcaaagct ctctgcaagg
    15541 ttgaaagtgt gtttatttcc ttttcctgcc acatatccca cataaccacc gccatagtta
    15601 taagactgta tcgctacatt caaatcgtcc ataccttgat ttttgcagga cgaaagcagg
    15661 gacgcaaaat atttacaccc ctgcttgatt gaactttctg tatctaatga attgggcggt
    15721 agtcccagac tttcagagct ttgcataacg tcctcggctg taccgccact ttccacttgt
    15781 atgattgcca gcaacacatt gacatactcg gaaatgccgt attctctggc gtacttttcc
    15841 accataggct gatgtttcaa gacttctgcg gataagttca tacccgtaat gccagaagaa
    15901 aagttgctgt tctcgtcgtc gctgtccgca ctaatcaaca ccccaaagaa aagcaccagt
    15961 gaaaagagga taggaaacag actgccgata atagcaatat gtttcagttt cattttttct
    16021 tctgtccttt cttcattaca aggttacggg ttttctctgt ggtctgctgg tttttctgga
    16081 cgctttgcgt ctgctgaact tttgtcctgc ggtctttggt gtcattctgg cgtgtttcct
    16141 gcgataccac tttttctaca ttctgcctat gtactggctg tgtaggctgg ctcgttttcc
    16201 tatcagaagt acccgaagat aagggacgct ctttgataac cgttgtcttg actggctcat
    16261 ttgtttttga agtcgtcgct gtggcagggc gtttgacttc ctgcgttttt tcagcactcg
    16321 gctttggaat ggttgaagct gtggctggtc gctcatgggg acgggtagct cccgttgtcg
    16381 ctgatccgtc agcctttcgc tgtgcctgcc ttgcttcttg tgccttttga agctccatac
    16441 gcttatcggc gatattctgc ctatgctgtt cccgtttttc catacgtccc gactgtctgg
    16501 actgctgttc ctgcaccatg ccacgcttga agtcggacac gctggacttt gccttttcct
    16561 ttgcggagtg gacggcataa gcggtctgtg tcggcatatc cttgatattc tctttgacag
    16621 cacttgcctt atctttcact ttattttttg tatctaagac agcacccacc tttgaaccta
    16681 cacgctgtcc catgctggaa gatgtattac cccgactatc ttttgtagct gtgttccttg
    16741 tcgcttttct gtttgaaatc gtgctaccag ccaccgcacc agcaacacca ccagcagtaa
    16801 ccgcaccagc aagccgtctt tccatacgtc tagccctatg tgccaagtat agatatggtc
    16861 tgcggaaaat tcttcgtccc atgctttgac tatcattagc gttcaaagag aacatactca
    16921 ttaagtcacc cagcttcatg tagataccag cgaaacacac tatctgtaag aacgccacca
    16981 tgaaaaacgg atagtccgtg gatatgttat aaaacatact ggaaatgctg aacgccaccg
    17041 ttacaatgag cgttattcct gctcgtgtca ttatggtatt aaacaccctc acgattgcct
    17101 gctttgccat gctttcatag ctcggtatca tggaaagtaa aaagctgata ggcagaaaca
    17161 tagcaaagat aataaaaagt atctggctga ataacatcat gcccgtaagc aagaatacaa
    17221 atatcgttat ccctaagttg aagaacagta ggaagaacac catacctaaa cggttcacga
    17281 cctgcggtat cgtcagattg ttgttgtcgt tgtcctcgat ttctgttttc acgacttcct
    17341 ctctggtctt tccgtcctcg tcctctgggc ttgccgatac cagagcttct acacggtctg
    17401 ccccgatttc ttctgcgttg ctgttcccga attgtaagag taaccacggc tgttccactt
    17461 gaatagaaaa taggctgtcc cttatcaagt ccacgctgtc cttgcccttg ctgtcagagt
    17521 ttgggagcat gatttttgtt cccaagtcca acgaagcggt actaatatca cttgaaaatt
    17581 catttatctt tttgatgtag tcgggagcgt aggcaataaa cgaagcggac agcacgaaca
    17641 ccaccacaaa gttaataacg gcatgaagtg ccttgctggt ttcccgtttt atcagtcccg
    17701 tataggcaac ataaagtccc accactaaga taatgagaag caggaaacca acatagaagc
    17761 ccgtagaaga aaaaccgttc tgtgttacgc ctgcaatggt ctgtatgctt tttccgatac
    17821 tgtctgccat atcgttaatg aagtccagtt tataggcttc ctgcaccaca tagcccgtgg
    17881 cattgcttaa ataaaggctt atcgtccaga caaagttggt aatgcagtaa agcccatact
    17941 gcaccgattt tccgataccg tccagccagt tccacggaag ccacgaccag ctattatcca
    18001 cataaaaatc aagctggtaa ttcgacagcg gatattttga gtaaagattt tctgcattta
    18061 tggtatcgtc cacaagcccc gtcgcatgag ccaccgtccc cagaagtgaa agcaggataa
    18121 gggagagtgc caccacgaac agaaccattt tgagaaagtg gaaaatcttc ttttttgtga
    18181 acgcaccttt tatcctttct ttcatcactc cacctcattt ctctgtacgg gcggtctggt
    18241 atcaaaggcg tgtagcagtt cttcaaagac ggggtgtatc tgcaccacac cgacacgtcc
    18301 gtataagtcc tgcaataagc attgtccgtt ctctaaatca cgcaagcgtt tctggttgtt
    18361 ctcgtcgtcc ttgtcgatac caaaaaactc taaggtctgt tttatctcgt taatgtcagt
    18421 agaacgaaag gcaaacttca agccgatatt gtttttcaga ctttcctttg aaacatcata
    18481 ggcagactgg gtaacaaaat aaacgcctgc ctgcatagct cgtccagcac gaaccagctt
    18541 gttagagagc gtttctcctt gtgccacatt taagaacgcc cacgcttcgt caaggtctac
    18601 aatcttaaaa atacttctgt ccgaatggat aaaatcaagg gcaaaggtac taatcacaat
    18661 cagcatagaa accgataata attcaatggt cgtgtattcc tcaaaagtgg tatctttatc
    18721 tggcagtaca aggtcagcta cttgaatgat gttgagctgg ttatccagac tgatagcatt
    18781 ttctaccgtc ccgtctgaaa acagcagatg tgcaaagtcg tagtccgtga agctgtcgat
    18841 atggtctgcg atatttcttg atatgggcgt atcttcacgg cgtagctcgt ctatcacatg
    18901 gagcaagccc cgactgttgc tctgggtaac ggagcgtacc gctttacgaa gtacggggaa
    18961 tttttcgccg tccctagagg aaatacccgt aaggaatgtt aagatgtcga ttgccagact
    19021 ttcagcgtct tttacattct tcataatcac gaatgggtca agaagtcctg catttccttt
    19081 atcgctggtt aagtttacga tattgatttc atgtgcgatt tctggaagtg tttccttcca
    19141 gttgccacgc tctgattttg ggtctaagat aaccgcctgt cccccaaaga gaaccgaata
    19201 atacaccaga agattgttac agaacgactt tccaccgcca agcgaaccga caaaagcaga
    19261 cgcaagggcg ttggtaactg taccttttat accttgactg gcaagggacg gttgcaggta
    19321 cacatttctt cccgtatcaa cggaatagcc catatagata cccgtagttt ctcctaactg
    19381 ctgtgtcgca ccaaagccaa gcccagctaa aaaatctgat tttacatact gcacataatc
    19441 attgatatat cgcttgctgg caggtaaaaa ttcagaatga agccccagca tatccccagc
    19501 aggacgcacc agctttacat tgaggtcgtc gtaaaagtcc ttgacttcat cacaacggcg
    19561 ttttaattcg tccaaatcat cagccgatac acgcactaca taagagagct tatacatact
    19621 ttcttttgtc tggtctaagt ccgtttccag ctcatccacg ctgtctaatg cgtccaccac
    19681 atttgaactg gtttcacttc ctgcttgata ggcgtgatta tccaaatctt tcaattcctt
    19741 tttcttattt cttactgtgg taagagcctt tcgattctcc acgatttcta cattcataga
    19801 agtatcaacg gggaatgtga attgctgttg ttggaaatag aagatttcac ttgacggaaa
    19861 atcaagctcc ccgacaatcg cattgacggt aaagtaggac acatagcttt ccttgtcctc
    19921 atgttccaat cgtaaatacc gctggctttc ctcaatcaca caccttgtcg gacggataag
    19981 gtcgtagtat tttatcagcg tttctttgtt caatttcttc tttggtaact gatactcata
    20041 atcttcatag gcgataccgt tcctgccgta aagatgttcc atgagatacc caaaatcatt
    20101 gatttccaag cgacgcacct tgaagcgacg ggagatttta ttttccagta acttttccat
    20161 tttcatgtaa cggttgattt catcattcgg catggaaaca aaatcattca tcagcgtgtg
    20221 gttcacttca tggagaaatt ccgtgaacgt cagccacgcc gattttttta tgttcttcag
    20281 attgagctgt tcttccgtaa ccatgagctt aaagccaaga aaaaagcggt agtccacttg
    20341 attgtcacca atcatagata ctaacgcttc ggtctgttcg tctattttct ggcaggcaac
    20401 ttcctttaat tttccagtta ccagcttctt tgactgctcc tgcatactat ggattgagct
    20461 ttccgtagca atctgcaacg catgaatttt accctcacgg gactgtgcga taagctgtcg
    20521 gaaactgtca tgcacgataa atttctgctc tgcggataga aaagaatagt tgtacggtat
    20581 cagctcatag taagcgaaca cctcattgtc cttgttccag acaaggttat tgtcaatata
    20641 ttttatcggg aacatagttc acactcctta ctgccgtgat tgtttcattc aaggtctgct
    20701 tatgcagttt tacggctttt cccgcataag tgattttagg tcgcagggca taggttatct
    20761 gtgatttcaa aaagctgtac ggcttctttc cgtcaaaggt tttctgcgac ataaaccaag
    20821 tgagagcaac gggaataccg aagtatttga gaaatgctcc ctcaatcatg gaaagtgggg
    20881 gaatatcccc aaacagaatg atgataaatt ccgtaatcac aaaccatgta atctgtgtaa
    20941 aggtaacggg aaaaggcagg ttaaagtcat tgattgcata caagactttt tccacgttcc
    21001 agatacccgt ataagattta atctttttca agttctttca gctcctttcg taagttcttc
    21061 aaagagaaaa ggacagccat tttcagactg tcccttaaaa gagaaatacg ctgtcagtag
    21121 caaccatgcc gttgctgatc ttccagcgtt aatatggtat ctcgcatatc cctcggcttg
    21181 tttcaataaa gcaaccgccc atatccaaat ctcgcccgta ggcttcataa tcaatgtagt
    21241 tctgtaagct gggtggaatg tccccaagtg cctgcaattc gtctatgtag taataggcaa
    21301 catctgtcat ggtttcacag tcgggataat aataaatatc gtccttgtgt tccacgactt
    21361 cttccagcgt cccgtagtga ctgataaatt cgtccagaca ctctacgata tagtcgggaa
    21421 gttcctctat catttcatac atctcattga gttcttcaat ggaaacatac tcgccaatcg
    21481 caatggggaa gttgtcggta tcatggatag cgtattcctc atactgttca ttcaagccga
    21541 ttttttcttt tacatcttcc tcgtcaatgg ggaacgtgaa ccaagcaccg actaaataac
    21601 cctcattgta tttaccgaga ttagcaatgt aaaccgccat atcatctatc ataagcacca
    21661 cctcatttcc tactctggta gttcaaagat accgtgttcc gttactaaga atttcccgtg
    21721 ttcctgcaag tgagaagcgt aggcttcaaa gtcaaagtag tattcttgac agtcctcgga
    21781 taaatgtttg aacgtcgagt cattcatcag cttttgcctt gcaacatcta tcatgctttt
    21841 gcagtcggga taaaccgtaa tcacgttcct gcaaatataa agtgcttcta aattctcaaa
    21901 caccgtaagt agtgatacat attcttcctg catatcgctt gaaagctggc ggtacataaa
    21961 ctctaattcg ttaagctggt acacgcttgt atgttcgtgg acttcatcag cataaggcaa
    22021 caccttttcg ataatgcggt acgccgtgct ttctgcaccg acacctaact tttcttcaaa
    22081 ctcggcaaca tctatcggca ggtcgaacca gtatgatgtt gtttcttcgc cagttgtttc
    22141 tctcgtttcc accagcaccc ttgtttcctg cattgttctt cacgtccttt ctgttgtttc
    22201 atcacatctt ttaaggtctg gtacggcata ccaaacacaa actttgaaaa atcttctaat
    22261 tgtctttctt cataatcggc agggctcagc cgtttcattt cacgccacac cttacgcttg
    22321 taagagggca ggtcagaaat gtattcacag ccgacttttg cctgcatatc cttaaaaata
    22381 tcgttcatgt aatcactcca atctgaaatt ttggtatgaa aaaagcagtc aatcataaga
    22441 ctaactgctt tgtgtgaata ttaaattata gcttatccta atctcttttt aatctttctt
    22501 tttagtttga aaaagaaact cgttgaatat tgctcgtttt tcactaatag ttcataggtt
    22561 tccataacat tgattgtata tacaaaaccc aacattccaa cagcaagacc gaaaaatgca
    22621 aaggaatgat attctgtcca tgaaatatag cctttcacag taaatagaaa gaaaattagt
    22681 cctgctataa agagaacagt acaaggaaca gcaactatta catatttttt cttttttgaa
    22741 gtgtattcgc ttttcaattc ttttagatat tcataatcaa gtagtaaagg ttcttgcctt
    22801 aaaatgttgt attcttgttt ttcataaaaa attgctacga caaaaaatat aattcctgcc
    22861 acaaagcaaa atgccatacc taaataacgc caagtcatat tatctaaaaa caaggtatat
    22921 ggtattccag caaaaatgca aagcataacc cctaaagcaa gataaagtcc taattttttc
    22981 tgattagcga taaaccctac cgacatttcc ttgcttacat aatatcctcg ttcttcacta
    23041 ttctttacat tcttttcgtc tttcaaaaga taatccgtag atacttcaaa aatgtttgat
    23101 aattgtaaaa gcttttctgt ttctggaaac ccttgattgt tttcccattt actaattgcc
    23161 tgcctagtag tgcctatttg ttcagctagt gcttcttgcg atattccttt ttcttttctt
    23221 aacttgaaga ttttttctcc taaactcatt gtgtgatacc tcctatgatt tgatgaataa
    23281 attttatcaa gccataatgt tatattctat attttttgaa atgcacatta gcaacttcaa
    23341 gttgcaacct tgtataatat acaaaattca tagatttttc tggtaagtac agcgttttat
    23401 gtaatacaac attcaaatta taacattcgc atatcagtac cacaataaaa ttttatgcac
    23461 cgataatctt attgaacagc tctaacaata catctttcac accgccagca ttgaatacca
    23521 aaccgacagc aatcaaggca actaccaaga agccgataag tttggaaaac tcacgcttga
    23581 accctaagta gataccgata accacaatcg ccataagcac taagctctgt gcgttactta
    23641 aaaaccattg atacaaattt tgtccgaaat tcatttaatt gtcctcgctt tcttctaatt
    23701 gtttcatttt atagtcagct tctttgccga cattcaaaat acacatcaag actatgccga
    23761 tacccattcc catagatacc agcaggaagt cttttaataa tacccacatt tttcatcact
    23821 cctaaatttc tactaaatct tttgcagggg tcgtctgctg tttgattatc atttcatgct
    23881 tttctgtgag ctttgcctgc tgttcgattg tctccatgta atctgtaccg tttcccttat
    23941 caatcttttt cagcattttc aaggtcggtg ctacctgcct ttgtacccaa cgcaatgtac
    24001 gatctaaggt ataaggttct ggctttgtcg tcagtttcag actttgccga ttatcgccga
    24061 taaaccaagc ccagcggtca ttgagtttcc agtcattttt ccgcttgtct ggttcttcat
    24121 caacaaaccg tacatactga ttgatgatag aaaaggcggt ctgctctgcg tcgtaatacg
    24181 tcagcaaatc ccgtactgca taataggcac gttcatttct aagccgtatc tcaaaacggt
    24241 tgataatatc ggcttcttcc agaggtgtcc ctaacttgac atactgctca tagtcctttt
    24301 catagataca gaaatataca tcagatttca gcgaaccaag ataaagggta cgtcccatat
    24361 attctctgtc gtcctcgctg tgcttgataa gctcgcccga ctggtaaaac ttataacttc
    24421 tggactttcc gatatattcc cgttttctgc atttttccgc aagctctgga atatccaaaa
    24481 tgcccgtatg gtcgttgata gcaaggtcga tacgcttcat cacgccaccg tctatgagtg
    24541 cgtccatgag aaagtcatac cagcttcttt gctgtgccag caggtaactc tcaaactgtc
    24601 ggcaaccacg cccctttaac tctaaaagga cacctttttc ttcgtcagcc gacgtataga
    24661 taaagatgtc ccctaaagaa taatgctccg tataactgta atgtccgtaa tcttcatgga
    24721 gcatatagtt gatattcagt tttaatatat ctttgatgat gtgctgtata tccagcgtgg
    24781 gaaaacgaat tttcacataa tcaaacagca tggtaagcgg tgcttctgga ttgaaccttg
    24841 ccagttcttt atgcagagtt tccagaagtt cctttgacgg cttcattttt ccgctttcta
    24901 tcttgttgag atattccctt gtgataccag aagccaccgc cagcctgcct tgtgagatac
    24961 cgtaagcaat ccgtttctcc cgtaattctt ttatccattg ttcttcattc agaaccatac
    25021 ccccaatctg tcaagtgtga agttttttaa ggcgacttca cagccgattt ttactgggaa
    25081 atcatgccag aagccttatg tttcctatgt ttttcgtcta ttgtctattt gcaaacgtac
    25141 ccctctgtta gataccgagg ggttttacct gctggcgtgg cttacgccac accagcaacg
    25201 gctagtccgt gccgtcgcct ttcgcttcgc acgtcgccgt cccgtcctgc cttgcttgtg
    25261 caagagagcc gatagtctgc aaaaagtcat gtcctttcgg gactaaaggc gtgtaaaatt
    25321 cgctgataac gcttgttccc acatcacaat agccacgccc cttgatacgc ttctggaaaa
    25381 actgcttttt cacatctgaa ccaaacagca taccgtaacc taattcgctg atacgcccaa
    25441 gtcccacgcg gaaattgaag ttatctctga taccgtccga gaaatacttt gcgtctggac
    25501 gctggcaggc aacgataagg aaatatcctg cttgccgtcc taacataacg attttcttta
    25561 actggctaag taagcccacg ctttcttttg tccccagcat ttcaaaaaac gccacatatt
    25621 catcaaagat aagaaagcag ggtggcagtc ccagataagc gtagttttcg cccgtcttat
    25681 agttcgggtg tcgcttcatt tcctcacttc gctgtaccat accctcataa aaggcattga
    25741 cgcaatcaat cattttttct ttggtgtgat acacattttc cataactgtc cctaagtctg
    25801 ctaagtccgc atttttcggg tctaagatgt aaaggacagc gttggtatgc agtaaggctt
    25861 caatgagcgt cagcagaaaa taggttttac cgccaccagt cccaccagca atcagagcgt
    25921 gagggagtgc gtcatattcc cagacaagat ttttcatcaa tttaagacag ccattttctg
    25981 cccgtacttc atcaatagta atgcggttcg ctatcatatc ataaagcagg gtatattcga
    26041 tataaccgtc atgtaaggtc ttgtcggtca gctcacaata taatccactt tccaatttat
    26101 cctctaaccg taaaagctgg tcttgatatt ttcccagcgt aatttcacag cggatatgaa
    26161 gcagtccctt ttccatttga taataaatct ttggaaacca gacgattttt tcccttgatc
    26221 tgctttgcag gtcagtaaaa aaaccgctgt cttgtacggt atcggcttca taccacttat
    26281 tttccagtat cattctcgcc agcttttgac ggtgcaggag cttcttgaaa ctgtcgtaac
    26341 agaagcggta atacagaaaa gcgaccaatg cacaaacacc agtcgctatc agtatggtta
    26401 tgaagttgta aggggaaagc gtcaagccgt tctctaacaa gctgaaatgc tcccagtcgg
    26461 tacgcatgag ctgtttgata ttcagtagca ggagaaccgc cacgaataaa aacagaagcg
    26521 tccctatgga aaaatggtag acaaggtgct tgtcgctggc tctgatacgg tgtcctctat
    26581 tccaaatctt acgcataaat cattcactcc tttctgggta cgaaaaaagc agtcaatcat
    26641 atagactaac tgctttgtaa atgttgcaag atataatcat aaatctgcat ttttagttat
    26701 attaaggtcg cacatataat gccaattaca ccaatagcca aaaataacag tccaatacct
    26761 gctgattttt tacgtttttc taattctttt gagttgcttt cactcatatt tacaataggg
    26821 tattttcttc ttccgattag atacataagc aaaccacttg aattattccc actcattgta
    26881 aatactcccc aaaatttagg gtgctttaat cctctggctg ttgcgtctat aacagtcata
    26941 tgataaatct gaaacacaac acaaaacgct cctacaagtg caactgctcc acaaattacc
    27001 gctaaaacta tgttcaaatt cataacaaaa tatcctcctt aaaatttttt gattatctgg
    27061 taaagtaatg ctgatgaagt gataaccata agacaaaata ttccaactaa gaaataacgg
    27121 ttatctggta taaacatatt taatgctatc agcaatgcta cgacaatgat atttacaata
    27181 attgcccata tcattttttg gttgcttttt acaacatttg tactttcttc taaatgttca
    27241 atcatttttt catcaccctt tagcaattcg tcaagactta tagaatatag gttgctcaac
    27301 tctattacat tgataatgtc tgggtaagat ttttcatttt cccagtttga aattgtctgc
    27361 cttgatacat tgattttttc tgcaacattt tcttgtgtca ttccagactg tactcgtgca
    27421 tttttaagtt tcttacctat ttccatattc cgattgctcc tttcggtgtc attgtcatat
    27481 attaaccctc aaatgtctat ccaatatctt cgacaaatcc tttttttcct tgtcaaaatt
    27541 attttacata gcttaaatac ggctcttttg gctcattata ctgtaatttc agagtataat
    27601 ctatgaccta gatttatttt ttcgtctgtc cctgcggtac atggttctgt ggattgcctg
    27661 cgttctgatt gcctttattc ttcaacacaa tatcctctgc ctttacatac cagtccacat
    27721 ctgcaccacc aaacgtcttt cttgataccg tatcggctac gggattgaca agctctacaa
    27781 ctgcattgta cggaaattcc cttaacggca cttctggcgg tacggacacg gggataattc
    27841 caccatgcag actacatttc aagtcataga tacgcttttt aagctgggta ctcggtgtcc
    27901 cgtcctcatt ctgcataaac acatcacgca ccgctgtaaa ttttaattcg ccaaatgttt
    27961 tctctttgtc aataacaaac ccgtttgata atctcataaa ttcttcactc ctttactttt
    28021 cttcaactgc tacaatatca tcagcaacta atacataatc ggtatgtccc atatccccga
    28081 ttgcgtaacc tctgccgtac aacttcggat tgacaagttt tactttctgt tcatacttga
    28141 acttcttttc gccagcctgc acgggaattt ccactacaac attttcgcct ttctgtacgt
    28201 cagaataaag gttatagctt cgtctggcta agaccctgcg gttattttta tctctttcaa
    28261 agataggctc gctttctcct gcaaattcaa gagttccaaa agactgtgcc atatctggca
    28321 cgacatattt catttccata atgttttacc tcgtttcttt ctattttgat aaattgattg
    28381 taatttctta ttctggcggt ttgggaagta ccagcaatcc cccagatagt aaagggtaaa
    28441 atcaatgctt cgcacccttg actatccgtg ttcttgcagg ttggtggcag acaagccaga
    28501 ataagcaaaa gtctgccgtt tgacagcttc ggcggagagc gtgatttttc tttcatcata
    28561 ccgttaccgc cttatgattt cttcatttta cggcgtttgt aaagatatgt tccagccagt
    28621 ccaccagcag acgcaagcaa catcacaaga aatgccatta catttgtact gtcgcccgtt
    28681 ttgggactgt cgctcggtct gctcggcttt tctggtatct gtggtgtttc gggtttctct
    28741 ggttcttctg gcttttcctt aaaggtaatc gtctgtccct tatcctcaat atccttatgc
    28801 tcggtaactt tcttcggctc gtctggattg cttaaatcgt acaattcttc aaaagttaca
    28861 agctgtttac cgtcaagaga agtagcgtca aacgtaaaag caacttccac tttcatagtt
    28921 tcgctgtcag cagtaaacgt ataatcattt tccacacatt ttccattgat aagaagttct
    28981 gcattttctt ctttcaacat ctgccagccc acaagtttgt actgtgtacc aacttctaag
    29041 ccctctaagg ttacggtatc aatgattgta acgttttttc cagcttcaag ctctttgttg
    29101 ccgtccttat tcgtagcagt cgtatgaatt ttgatgatac gctctgtgat taaaacggtc
    29161 tgcccgtcgt cctcaatgtc tttgtgttcc gcaactttca cgggttcgtc tggattgctc
    29221 aaatcataca attcttcaaa ggtaacaagg ttcttgccac ctaaagcaga cgcattgaat
    29281 gtataggcaa tttccacttt catttcttcc tcatcagcga caaaggtata atcattttct
    29341 gcacgtttcc cgtcaatgat aagctcggcg ttttcctctt ttaacatctg ccagcctttg
    29401 agctgatatt ttgtgccttt tgtaagtccg tctaatttga cagtatcaac aatcgtaatc
    29461 tcttttcctg caaggatagt cttttcgccg tccttgctgg tcgctgtggt atggatagag
    29521 atttcttttt cgtattcatc agtcagcgtt cctaaatcaa tcaaaagatt atttcttgat
    29581 accacgattt caaaaggtgg gataagttca aagcctttgt tgctttcaga gcgtaattcc
    29641 tcaatgacgt aagtatcgta aggtaacgca cctttgctgt cgtctggttc agaagttcca
    29701 aaccacacac cgtcctcgct ggtcttgcct gcgttggtat tgtgcttatg agaagcccag
    29761 tcagcagaag tggagaactg cccgttattg tcagttacca cgatatgact ttctcctgtc
    29821 gtcttgcttg tgatcctaaa gggaacatca gcaagacgct tgtgtgtgcc tgcaccgatt
    29881 tttacaccct caatatctcc acgcttaatc tgattataga tagaatgggc tttgtcggtt
    29941 aagtccacga tttttccatt ttctgtgatt gtaaaatcaa tcggctttgc accgtcagtt
    30001 aagtaaccgt cgggagcttc actctcaaca atacggaatt ttccataagg taagaggtca
    30061 gcagaagtag aagcgacacc ctcaatatcg gtacgaattg tctttaccac ttcatttttc
    30121 ttgtatagct tgccctcaac caaaacagaa ttatcattta aggaaatgat gtcaaaagca
    30181 gtatctttca aggttgcact tccttgtggc ttcatatcgc ccgtttctaa atctcgcttc
    30241 tgaattttca caccgccacg gataaccttg tctgatacgg aaaactggtt acttccagat
    30301 aatacggcaa ggtcgtcgtc ctcggtaatc tgtgttacat ataagccctt tatctgttcg
    30361 gacttatcgc cagcctgcat atatgcaccc tctaacaagt agccgtttgg agcttttgtt
    30421 tcttctatgg ttagtgtccc aagtggaaga acggcttttc cgtcttgcat atagaagctg
    30481 tcgccagaca ccttgtatgc gtccgctaat tttgtgatgt aatgagttgt cccgtagctg
    30541 tctgtttcag cgattgtctt tgtaacccat gttcgagtag cttcggcagg gagattgtct
    30601 ttgttataga agccagcata atacttccat gtaaattccg cacctgctaa agaagcgttc
    30661 ccttgcggat tgtctttctt tgtttccata tctatcttga aaagctcaat caaagtgtcc
    30721 gttacttttg gcgtatcaga tacgttcaaa gtcgccgtct ttccagcttc aatctttaag
    30781 gaatatacag ttttatctac tttatatcct gctggtgcgg ataattcctt gatatagact
    30841 gtgcctgctt ttacctctgc aacatctgta tttccgtttt catcagtcgt aagggtggca
    30901 agctgtttcg tgcagtcctt atcagtaaag acaccatagg tcgcaccagc gattgagtaa
    30961 ttcccgttac cgtctgtaat gctggcatta ctggaagtct ttttaagttt cgcatttcca
    31021 acattcagct tcgcccagaa ttgccccaat tcctgcccct cgccagagta gatataaccg
    31081 ccacattcat agcgtccttt attttctttg acaaaggttc ttgcaccaga gaaaacttcg
    31141 tcctgcgttg cctttggaat ttcatcataa gaagctcgca cgttatcgca ttgccagcca
    31201 agatgtacgc tcaatctctg ccagactaca cattgttcca acagatagac ttgcttgtag
    31261 ttcagttcct tgtgagcttc gccatactgt ttgacatact ctaaggataa cgccacatct
    31321 gaaatctggt tggcattcat gcgtgagctt gcgtcagctc tggtcttgta gccattccta
    31381 aaatctgtgt taatgtcgat acaataggct gttttgccct caactttcat catgccctca
    31441 ttgaatgtcg agccgataga accgtcattc attacttttt caatgatacc gacacgctct
    31501 gcactttctg tccagtattg cttactttct gcatgaacgg gtgtagtcgg taaagcagta
    31561 acgatagttg caagagctaa gaagcccgta cacaatcgtt tcaatgtctt tttcataaat
    31621 tttaatactc ctttcatttt tggtataaaa atagacgctc atttctgaac gtctataaca
    31681 tacgagtatc ttgatttcta gtgataaatg atttatagtt tgtttgtaat gttttctgat
    31741 aaattaaagc tttaaattag cctttgttgc taacaacctg ctaacactct acggtgtatg
    31801 agggtacacc agagagtatc aacaattaaa cataacaagc acttatgctt taatttacgg
    31861 cattaaacgc tcccaagagg ataatcgaac ttctagctac cacggttcgc agagaactcc
    31921 taattctgaa gcataagata cagttggata tattgtagac accaccatac ttaaaatcat
    31981 aattaaacta attaataatg cctttttatt tcctttcttc atataaattt tccccctcta
    32041 ggttatctat agtttataat cttccattaa tatcgtatat aaattaattt ttactaaaaa
    32101 attatataaa aaaagctatg aaaataaata aatttatatt catagccggt tgcaccactg
    32161 actcagtata tccataaggt gcccaataat agaatatact tcaacatctc tataatattt
    32221 cataaaaaca attaaaattg ctttatttat attaaaaata caattatata cttctattat
    32281 attatgcctc tatactattg tctatctttt ttacattttt taccttttat gagattaatt
    32341 aataattaaa taaaatcctt atctacaaca attacacaat tatattcagg atttatctcc
    32401 ttaactaatt ttgttatatc ttcttttaag tcatcctctg acatgtcttt ggaaaattcg
    32461 gaaggattaa ctactatatc aaatattaaa ttcttcttgc ttccttttcc aactattcta
    32521 aaatcatgca tagattttac aagactattt ttcttaagta tttcttcaag ttcttttttg
    32581 gctgacataa cttctttatc ttcaagacat attgggtcca tgtgaattac tagatatata
    32641 tctaattttt cagatatttc tctctctgca gtatctataa tttcatgtat ttccattata
    32701 tctatgttgg atggaatttc tgcatgtata gatgcaatac atctaccaac tccatagtta
    32761 tgaataatta aatcatgtgt acctattata tatttataag ataacaatag ctctataata
    32821 gaatttacca tttcttcatc cggtgcttct cctaaaagag gatttatagt atcttttaca
    32881 agagagaaac cagcataaag tatcactaat gatactacga taccaacata tccatctata
    32941 ggaaaattag taaatctagc tacaataaat gatataacta cacaacttga agtaaataca
    33001 tctcctaaag catcaagtga aactgctttt aaggctgaag aatcaatttt atttcccaca
    33061 aatttattaa aacgactcaa ccagatttta atcattatag atacaattaa aagtataagt
    33121 ggtattactt caaattttac aggaattgga tttactatcc tttctataga tgactttaca
    33181 aattgaagtc caaccaacat caccataaat gctactatta atgctgataa atactctatt
    33241 cttccatgac caaaaggatg ttctttgtca gctggcttac ttgctaattt aaaacctatc
    33301 atagttattg ctgaagatgc catatcagaa agattattaa atgcatcagc cattatagct
    33361 atactagatg tgaacattcc tatgaatacc tttatcacaa ataaaagtaa attggacaat
    33421 atacctacta caccagctac ataaccatac ttatttctaa catctgtatt ttgaacgttt
    33481 tcgctatctc tgataaatgt tttgactagg attttggaaa acatgaaact tacctccctg
    33541 ccctttattt tattaacaaa ttttataatc tatatcttta tttatgataa cataatttat
    33601 atataaaatt cttaaaaatc actctacttc agtaaaatat tattctatat atctatttag
    33661 atacaaattt tatatttcgt aaagttttta tggcttagtg tctgtttaat agattctata
    33721 taagttttta caaagattat ttattatgat aatatcattt ttcccaagta ttacttagaa
    33781 tatgattttt ttgttttgtc tttacatatt ttaaatttat actatattta tgtttttata
    33841 accttatgat acactataac tataagattt taaacggaaa ggaatgaaat aaatcatgtt
    33901 ttttatcagt gatatttaca caaaatcacc tattaaattt gatactccat tgcaaaaaga
    33961 ggtatacaaa atattacaaa agttagatat tgactttgaa cgtgttgaca cagatgaagc
    34021 aattacaatg gaagattgtg ttcagattaa taaaaaatta aatatgaaaa tggtaatata
    34081 aaacaacatt aaacaaacaa aaaccgcaaa gttacaatat atagtacttt gcggtttcat
    34141 aagaattaat ttttactatt agagttcata cttctactat ttttcattgt tctaagtatt
    34201 agctttacaa attcatttga caataatata gtggatgcaa atagtattat ttttatccac
    34261 atatcaaagt ccaaagaaac agaattaaag aaactctgga acacttgtgt aaatattatt
    34321 tgaattatcc ctgtaactac tattatttgc aatgccagtt tatttttaaa gaaatttggt
    34381 attgtactat ttaaaccaaa ttctctacaa tttaatgcat taaaaagggc actgaacgca
    34441 aataaagaaa atataacagt accttgttct tctgatgtag ctcccaatat attaaaagct
    34501 gattgagtaa atacaattgt aattataaga atagcattta taataatatt tacaaacata
    34561 gacctagcta ttattccaga atgtctattt attggttttc ttttcaatac ataatctcta
    34621 acaggttcta atcctaatgc tagggctgga ggaccatcca ttattatatt tacccataat
    34681 aattgtattg tagtaaatgg catatcctta ccagtaagtt gagatattat agcaattata
    34741 aatgctacta tatttacagt aagctgaaac tgtataaatc tttgaaagtt ctcatatata
    34801 cctcttcccc attttattcc ctctactata gtactaaagc tatcatcagt caatattata
    34861 tctgctgcat ttttagatac ttctgttcct gaaataccca ttgcaattcc aacatctgct
    34921 tgactaagag ctggggcatc atttatacca tcacctgtta cagctacaac ttcgccactt
    34981 ttttgaagcg ctgaaactat tctcattttt gtatctggtt tacttcttgc aacaatagat
    35041 attcctttaa tttcttctct cagttcttta tctgtcaata catctatata tgttgcttca
    35101 actgcctttt tcccatcatt taataatcct aattcatttc caatagccgt tgctgtattt
    35161 atattatctc ctgtaagcat ttttacatct actcctgcat taaatgcttt atcaatggaa
    35221 tctttgaccc cttctctcaa tgggtccact attcctacaa atccactaaa aactaaatta
    35281 tcttccttca tataacttct tgaatttcct attaaattca attcaccttc tgaagtcatt
    35341 gcaacttctg ttttagaatt actcattttt ttataagcaa atcctaatgt tctcatagat
    35401 ttaatttgta acttcttaat ctcatctaaa atactttttt ctacttttgg agttattgga
    35461 acaatatttt ttccttgttg tacataagaa cattttttta ataatacttc tggagcacct
    35521 tttgaaagta atatatctga atcctcttga cgaattaatg ttgacatctt cttttcttct
    35581 gaactaaatg gtatttgtga tatcaaatac gtttgttttc tcatttcatt atagttctta
    35641 tcattatgat ataaaagtaa tgcacactct gttgcacttc ctatatattt aaatgaatta
    35701 tcttcttttt ctatatctgc agttgaattt actatacagt tttgttcaaa atagctgtta
    35761 ctttgatact ctcctccact tatatattta ccatcaacat atgctacctc taccatcatt
    35821 ttgttttgtg ttaaagtccc tgttttgtcg gaacatatta cagaaacaga acctatagtt
    35881 tcacaggctt ctttctttgt tactaatgca tttatctttg ccatcttttg cattgttata
    35941 gctaaggtaa tatttatcat agttggcaaa ccttctggaa cagttgcaac tattaatgca
    36001 atacaaacca tatatgcatt tttagcaggt tctaatgatt gtaagaaaga taatatactg
    36061 cttgtatcta aaattaacat accttttctc tgcatctgta taatcataaa catacacaat
    36121 aaagtagcta ttacgcctga tattcctgct attttagcac ccaacttccc taatttaatt
    36181 tgaagtggag tttgctggtc ttcatttgat aaattctgtg ctatcttacc catctctgtc
    36241 ttatcaccag tatgagtgac aaccattatg cccctaccat atgctacaag agtccctcca
    36301 aataccatat tgacttgctt tgcaggtatt gcatcctgtt cgattatttc tgttttagaa
    36361 taaactactt ccatatctaa tacagcatca gcattcttag ctacatcatc agattctcct
    36421 gttagcatat cttctctaag tttcaaattt atagactgta taagtcttcc atctgctggt
    36481 atcatatctc cagtttctat atatacaata tctcctggta ctaaatcatt ttttgatatt
    36541 ttaattatct ttccatttct aagtactttt acttctatat tttctgttaa ttttgacagg
    36601 gcctgagctg cttttttaga ttttccttca gttattatgc ctatagaaat tccaataaaa
    36661 atagccccta aaatacctaa tgcatcatgt aattctccca caaaagaact tattactgct
    36721 gctcctatga gaattaatat cataggttct gtcaaacttt ctcccaactc gtcccaaaat
    36781 gttctacctt cttttatagt aaattcattt aatccatatc taagcttcct ttcttctact
    36841 tcattatcat caagtccaat ttctggattt gtttttaagt attttaatac ttctttggta
    36901 ggtttattat aatacctcat aactttatcc cccttgttaa ttatctataa ggaattatat
    36961 gcttatgata gcttgtatat tacttttcaa aaaaaataag gtgttgatta atcaacacct
    37021 tatagtggtt atatattatt aaattttatg ttacaggctt tataaattgt gagctataca
    37081 aatcaaaata tttaatcttc aaaataccct tctggatata atgggaactt agatataagt
    37141 tcttgtgcct ctgctttaca ttcatctaat acttctttat tatcaatatt ttctgctacc
    37201 ttattcatta ttcctgctat ctgtataact tctttttctt tcagacctct ttgagatatt
    37261 gatgtaagac ctatacgaac accactagtt acaaatggtg attgtgggtc gtcaggtata
    37321 acatttttat taactgttat tcctacatat tcaagagctt tttcaaattg ttttcctgta
    37381 agattcttac ttcttaagtc aaccattact atatgattat cagtacctcc acttacaata
    37441 ctaaaaccat gtgaaatcag ttcagaagct agagtctgag catttactac aacttgctcc
    37501 attatctttt taaatttatc tgtacttaaa tataatagtg aaaaggcttt tgctgcaact
    37561 gtattaagat gtatagaccc taatgttcct ggaaaaacac ctttatcgag tttctttgca
    37621 tgttctgctt tacaaagtac aataccactt cttggtccac agaaagtctt tgtcgtagat
    37681 gaagaaacaa aatctgcata aggtacagga ctaggtatta ctttcgctgc gactaatcct
    37741 gcaacatgtg ccatatctac cataaagtaa gctcctactt cttttgcaac ctttgatatt
    37801 ctttcataat ctattaatct tggatatgaa cttgcccctg caattataag ttttggtcta
    37861 cattcttttg cctttgcttc aagagcatca taatctatac gtccagtatt tgggtctacc
    37921 ccataaaatt catatttata aatgtcatgt aaaaaattaa ctgcgctacc atgtgtcaag
    37981 tgtccacctt ggtcaagacg catacttaaa acagtgtcat ttggttttaa tattgatgaa
    38041 tatacacaat agttagctgt agaacctgaa tacggttgta cgtttacatg ttctgctcca
    38101 aatacttctt ttgcacgttt tattgctagg gtttcaagtt tatctgcttc ttcagaacct
    38161 gcttgaaaac gagcccctgg atagccttct tctgttttat ttgtaaatac acatccactt
    38221 aattcaagta cttctgttgg tgctgtactt tcagaagcta tcatctcaat attgtgttct
    38281 tgtctaacca actcatcagc tacaatcttg tataactctg ggtctgaaat ttttgttgtt
    38341 tttaacataa taatcctcct cttaattaaa gaatatattt tattagttta atcttcattg
    38401 ataaatttat tataatggtc tataaaacgt aagatattcc tccaccaggt cctaatggta
    38461 tgccaatacc catgaatagt aacaatatta ttatttctat taatgtaaat accactgagt
    38521 atggaatcat attcgatatg acagtaccta tacctatatc tttatcatat cttctggcaa
    38581 aggccaatat aactggaaaa tacggaaaaa gtggacttat tggatttgtt gaagcatctc
    38641 caatacgata agctatttga gttaatgctg gattaaagtc tagtagcata aacatgggaa
    38701 caaatatagg tgctaaaatc gcccattttg cagaagcact tcctacaaat aaattgataa
    38761 atccagatag caatataaat cctattatca atccatagct aggtatcccc gactctgcta
    38821 gtaattttgc tcctgttatt gataaaattg ttcctaagtt actaagatta aataaattaa
    38881 taaactggga tgctataaat gctaggacta tatatccacc catatcactc atactactac
    38941 ccatcataga tactaaatct ttgtcacttt ttatcttctt acttactttt ccatacacta
    39001 atcctggagt taaaaatata atagtgatta taggaactat acctttcatt aaaggtgcat
    39061 tactagatag tatagaacca gtctctggat ctttcataaa tgcattgtca cctatacata
    39121 aggcaactat cactactact acaactaaca atgataataa agcgtatttt atacccttat
    39181 tttcaagttc actcaattcc tgtatatcct tatcatttgt tgaatcatta tattttgtaa
    39241 aacgaggagc tataaatttt tcagttacaa atgctcctgc aaaagttaaa actatcgctg
    39301 atataaaagc aaaataaaga ttcattgctg gattgccttg gtaagatggg tccatcattt
    39361 gagctgctgg tattgtaaat ccagctaaca atacatctgt agtactaagc ataacatttg
    39421 ctgcaaatcc ccctgcaaca cctgcaaaag ctgcaaacat acctacaaga ggatgtcttc
    39481 caattcctaa aaacacaagt gctgcaagtg gtggtaatat tataaatcct acatctgctg
    39541 ctgtatgtga taacatacct gcaaatacga ttgctacagt tacaagtttt tgtggaactt
    39601 tacttatacc ctttttcatt aacacttcca taaatccaga tttctcagca acacctgctc
    39661 caagcattgt aaccagtact aatcctaaag gagcaaatga ctggaagttt gtaactatgc
    39721 tacctaaata tgattgaatc tgctctttct ctagtagatt tactaccttt acaacttctt
    39781 ttgtagaagg atgtatttct tctactccta aattagatat aactgctgac aatattacaa
    39841 caattataca tagtaataaa aatatggtta ctgggtctgg aagtttattt cctgctctct
    39901 ctacaacatt tagtgccctc atcaataatc cactatcttt gtttttttcc tgtgaattaa
    39961 ttttcctcaa actaataccc cctcataaac tttatctcca gaatgctcta tcttaaattt
    40021 tattttttaa tttatccata taatttataa cagactgaca tagtacattt gtacctacca
    40081 atatagaatt tttatcagct ttaaatttag gattatgcca ctgatatata caatcttgtt
    40141 ctttattgcc tacccctaac cagtaaaaaa atcctggggc cttttccata aatatagaaa
    40201 aatcttctcc tcctgtagaa ggtattggat caactatagc atcttctgaa tacaaatcac
    40261 acacagactt acaagctatt gtgtacatct ctttactatt tataacagct ggtaaatcaa
    40321 ataaataatc tagctttgca gttgcttgat atccacgagc tatctcacaa actatatttt
    40381 ctaaattttc tgatattttc tttcgtactt cattactaaa gcttctacat gtacctgtca
    40441 tatctacctt ttctgaaatt acattatttg cagtccctcc atttatagta cctattgata
    40501 taacagctga ttctaatgga cttatgtttc tgcttactat ggtttgaata cccattataa
    40561 ttgcacttga tgctactata gggtctattg ttctgtttgg tatacctccg tgacctccaa
    40621 atccatttac ttctatctta atagtgtcta ctgctgccat taacccacct aacttaacac
    40681 ctatttttcc acaaggtata tctggatgat tatgaagtcc aaatatagca tcaacttttg
    40741 gattttccat tactcctcgt tccactagca tcttcgcacc tttattgact tcttcggctg
    40801 gttgaaatat aaactttaca tttccatgaa gactatctct aatatgagac aatacatatg
    40861 cacatcctac aaggcaagat gtgtgtatat catgcccaca tgcatgcatt ataccatcat
    40921 atctggattt atactcaata tcaacctctt catatattgg caatgcatca atatctcctc
    40981 tcagtgctac tgtgggacca tcatacttcc cttttaataa tccaacaact cctgtttcca
    41041 ttcctaaatc taatatttct atgtctaaac ttactaataa atcttttata gcttttgtag
    41101 tcctaaattc tttaaaactt aattcggggt gcatatgaat atctgtaaaa aaatctaaaa
    41161 tatcttcttt atacttttct ga
//
LOCUS       DACTGW010000008        36347 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.008, whole genome shotgun sequence.
ACCESSION   DACTGW010000008 DACTGW010000000
VERSION     DACTGW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.008"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            175..2094
                     /locus_tag="I9573_000355"
     CDS             175..2094
                     /locus_tag="I9573_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT4794993.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQERFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISKEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            2107..2394
                     /locus_tag="I9573_000356"
     CDS             2107..2394
                     /locus_tag="I9573_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4794994.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            2527..3786
                     /locus_tag="I9573_000357"
     CDS             2527..3786
                     /locus_tag="I9573_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAT4794995.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISKFIGSLFGGIL"
     gene            3789..4529
                     /locus_tag="I9573_000358"
     CDS             3789..4529
                     /locus_tag="I9573_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAT4794996.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            4852..6162
                     /locus_tag="I9573_000359"
     CDS             4852..6162
                     /locus_tag="I9573_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAT4794997.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            6260..7177
                     /locus_tag="I9573_000360"
     CDS             6260..7177
                     /locus_tag="I9573_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAT4794998.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            7193..8002
                     /locus_tag="I9573_000361"
     CDS             7193..8002
                     /locus_tag="I9573_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAT4794999.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            8059..9807
                     /locus_tag="I9573_000362"
     CDS             8059..9807
                     /locus_tag="I9573_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HAT4795000.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKEKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSTISWKGLENRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLNIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDKSKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMSKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDENDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIENLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            10070..11092
                     /locus_tag="I9573_000363"
     CDS             10070..11092
                     /locus_tag="I9573_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HAT4795001.1"
                     /translation="MFGGINIKRIGIIIMFFLSIAFIGFYLAIRGNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEEFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNVPKDSNIVV
                     SFHYYEPNDFAFQGNIYHNGFEHLSNITWEGTNEQMDYLKKRFDIVENWANKNKVKIF
                     LGEFGVTKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            11097..13403
                     /locus_tag="I9573_000364"
     CDS             11097..13403
                     /locus_tag="I9573_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAT4795002.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPVISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKNKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKIRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIALEINENIVLECDLCEGKIDERGLLYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTIPVSNPSEKKEKSIDEFEPMEYI"
     gene            13375..14490
                     /locus_tag="I9573_000365"
     CDS             13375..14490
                     /locus_tag="I9573_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795003.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSAKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSRITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQKINLSIQSKNMLEFRLKEGSEIFLEDKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEKSNVVLDSSMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     SFLHNIKITGNEEVQIPVLGQSISQYEVLGLKRDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAEPGQIILQVNVDDKFKIGRKIALTESWSINKKDKDGVPYTLVSPEQSPTVQYMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            14508..15926
                     /locus_tag="I9573_000366"
     CDS             14508..15926
                     /locus_tag="I9573_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HAT4795004.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCVYNIGVLFIFKYLAFAL
                     RNISELINTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTMQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMLGG
                     NGVIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            15941..17149
                     /locus_tag="I9573_000367"
     CDS             15941..17149
                     /locus_tag="I9573_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795005.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNICYSGKDMIQEIKNSVAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSEEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKENYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKYSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCK"
     gene            complement(17230..18618)
                     /locus_tag="I9573_000368"
     CDS             complement(17230..18618)
                     /locus_tag="I9573_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HAT4795006.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFTIHLIFITLNGLNPITYIRNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSLYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFGKKNIS"
     gene            18960..20294
                     /locus_tag="I9573_000369"
     CDS             18960..20294
                     /locus_tag="I9573_000369"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017826042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAT4795007.1"
                     /translation="MRVVIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(20423..21340)
                     /locus_tag="I9573_000370"
     CDS             complement(20423..21340)
                     /locus_tag="I9573_000370"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAT4795008.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLNSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            21548..22432
                     /locus_tag="I9573_000371"
     CDS             21548..22432
                     /locus_tag="I9573_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HAT4795009.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRIKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGKKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            22645..24486
                     /locus_tag="I9573_000372"
     CDS             22645..24486
                     /locus_tag="I9573_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAT4795010.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKNKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDYVVSKKVDEIKEQQEKILDKVIGATTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNQGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            24769..25497
                     /locus_tag="I9573_000373"
     CDS             24769..25497
                     /locus_tag="I9573_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4795011.1"
                     /translation="MMMEDIGMNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEA
                     KNIFAKEEICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGA
                     DDYITKPFSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEI
                     ILSKTETKLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNP
                     SEPKYIKTVRGVGYMWGERSIKKC"
     gene            25491..26801
                     /locus_tag="I9573_000374"
     CDS             25491..26801
                     /locus_tag="I9573_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4795012.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMAQE
                     NYLKDTTESEETGYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEKLEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            26918..27601
                     /locus_tag="I9573_000375"
     CDS             26918..27601
                     /locus_tag="I9573_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4795013.1"
                     /translation="MKILYTENLSKHYGKGENLVRALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            27594..29981
                     /locus_tag="I9573_000376"
     CDS             27594..29981
                     /locus_tag="I9573_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAT4795014.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFELIKGKLPTKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKNDK
                     KIEKEFVISGILKTSEISEAGKYYYAIISELYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGGFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKAVISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            30260..31402
                     /locus_tag="I9573_000377"
     CDS             30260..31402
                     /locus_tag="I9573_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HAT4795015.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIVGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLDRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALKLTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNENLLNLFNKYYF"
     gene            31421..31945
                     /locus_tag="I9573_000378"
     CDS             31421..31945
                     /locus_tag="I9573_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAT4795016.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYITEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            32045..32863
                     /locus_tag="I9573_000379"
     CDS             32045..32863
                     /locus_tag="I9573_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT4795017.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSELFAKKLEESLNPTQSVEKIVLYLEEMLLSILEEDLISNKSV
                     KLLNSFIYDNRYSNIKAFCDNTNINIKTLERICLKYTGYTPKILKRIYRFKMASSQLI
                     YNYKDNELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYNYL"
     gene            32941..33252
                     /locus_tag="I9573_000380"
     CDS             32941..33252
                     /locus_tag="I9573_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795018.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(33555..35558)
                     /locus_tag="I9573_000381"
     CDS             complement(33555..35558)
                     /locus_tag="I9573_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan-branching enzyme"
                     /protein_id="HAT4795019.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(35711..36343)
                     /gene="recX"
                     /locus_tag="I9573_000382"
     CDS             complement(35711..36343)
                     /gene="recX"
                     /locus_tag="I9573_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAT4795020.1"
                     /translation="MITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNEIN
                     EEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKSYN
                     LVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENAMY
                     LAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        14114 a         3922 c         6192 g        12119 t
ORIGIN      
        1 tttatttgta taaataatat aggttatttt caaatttttt taataatttt accactgtct
       61 tcttttagat tagggttaga ctgtgcaaaa ttataaaata atactaaatc atgtgcaatt
      121 atttggtatg gcactacatg tagaaaatat agataataaa attaaggagg tagtatgaag
      181 gaaagggcta aaaaaatatt agattttatt attaaaaata acaatgtaac atcacaagaa
      241 ctacaagaaa ggtttaatgt ttcaaagaga actatttatt acgatattct agctataaat
      301 gaacaactag gaaaaagtgg taatataaaa aatgtaaaac acaaatttat atttgaaggt
      361 aatttatgtg atgcaaggaa aataatttca actgaagaag ataaattttt agatagtgat
      421 tacagaaaaa cttttatatt aaacaagata ttacttggcg aaaaaatatc aatagaaaaa
      481 ctaacaaatg aaatgttact ttcaaaaaac acagtagtac aaacaataac tgatgtaaaa
      541 aaataccttc aaacaatggg gttaagatta gagtataagg gaaagtacaa aattataggt
      601 gatgaatacg ttattagaga gctgtttctg ataattgttc aagaaaatgt actggaaatt
      661 aattctataa gtaaggaagt aagtagtttc gatacaaaag gacatataaa acttactgat
      721 tattctttat taaatctaac taagtttgtg gaatttctaa ataaaaggat aagagatggg
      781 aaaacattat actcatataa atatctaaat gaagctaaaa aaattagtta ctttagtaat
      841 tgtaaagaac ttttatgtga agaagccaat gaaaatgaac aagcctatat atgcacttac
      901 ataagttcac ttcctagctt aaatagtgaa gttaaggaag atgttgttga agaatatgta
      961 gataaactta tagataagtt tgaagtaaat acagcaataa aattagaaag taagcatgag
     1021 tttaagaaaa atattttaag gcacctccac tcttcttaca acagaatacg atttaagttt
     1081 cctattagaa atccaatgtt agatgagact aagtataaac atgagtcact ttataaaatt
     1141 ataaaatcaa ttatagaaaa tgaagaagag tttcctgtat ttgaggggat aagagaagaa
     1201 gagataggat ttatagcagc gtattttgga gggtatttaa gagggtctag ggataatggc
     1261 ctaagaagaa ataaagtctt actagtttgt cctaatggtc ttatggtatc aaagagtttg
     1321 gaaatacaac tatataaata cataccaacc atagaaatag taggtatagt gtccattaag
     1381 caactaaaag aagtaaatgt atactacgat tacataatta caactataga tatacaaaat
     1441 gtaaacaatg taattgttgt aaatcctctt ttaacatcat cagatgttca attacttatg
     1501 aacaaactaa taagtgtaaa agaaaacgag aagtatttca atcttgaatt aataatacaa
     1561 gctataagaa aaaatggagt tatcaataat gaagaagcac taaaagctga tttattaaac
     1621 ataatacaca agatagatga aggagagatg tatcaaccaa tgttaaagga attaattaat
     1681 gcagaaaggg tcaatataat aaaaagtgtt agagattgga aagaagcaat aaaaatagcc
     1741 tcaaagccat tgttagaaga taactctata gaagagttat atatagaaaa tatgataaag
     1801 tcagttgaaa aatatggacc atatattgtt ttagctgata gatttgctct accacatgca
     1861 agttctaaag aaggagtaaa caagcttgct atgtctttac taatagtaga agatgaagtt
     1921 gatttattag gaaaaccagt aaatatattt atggtattag ctgctgtaga taacactact
     1981 cacattagag cattagcatc actatcagag atgatgtacg aggaagaaaa tgtaaaacta
     2041 ataattaatg gtgataaaag ttcaatacta gaacttataa ataagcaaaa ttaggaggaa
     2101 tgaaaaatga agatattagc agtatgtggt tttggtgtag gttcatcaat ggtattaaaa
     2161 atgacaatac aaaaggctct aaagcagtta ggtatagaat gtgaagtaga aaatacagat
     2221 ataaattcag ctagaggtgc tgattgtgac tgtatattca caagtcaaga attgttaaat
     2281 gaattaaaag gaacttgtaa tgtagatgta tatccagtta aaaagtacat ggatttagca
     2341 gaagtcacag aagttgttca aactttttta agtgataaaa atgataagaa atagtataag
     2401 ttttaaaaag taaatttaag ttaatcagaa taaaataaaa acttactaaa ttcaaaaata
     2461 gtaagaaaga gaggagggat ttagtggtag aaaacttaaa cagttgacct atcactaaaa
     2521 aatataatgc ttgattttat ttcaaataat attttacgta atccatctat gttaattgga
     2581 ttaatagcag catttggtct tgtactacag aaaaagaaag catctgatgt tataaagggg
     2641 tcactacttg cagctttcgg aattataatt atggagactg gaacaggtat gttagttagt
     2701 tcaatagcac caattaatgg agctttccaa agtataagtg gtggtgcagt aacaaaaggt
     2761 ttaagtgatg ttacttttac tgcaagttat ggtggaatta taggtttagc aatgtttgcg
     2821 ggtttagcta ttcacctaat gatagcaaga tttactccta ttaagacaat cttcttaaca
     2881 ggtcacatgc tttattggtt cccattcata tttgtagctt caggagttga agctggatta
     2941 aatggtggag tattaatagc ttttgcagca gtattatcag cactctattg gtcaataatg
     3001 ccttggttat taagaaaata tgtgtttgat gttataggag atgattcttt tacattaggt
     3061 catccatctg gttgtttagc acttatatca ggatttgttg ctaaattagt aggaaataaa
     3121 gaaaaatcta cagaagatat aaacatacct caaagtttat cattttttag agaaatatct
     3181 ataagtggtg gaatggcagt gtttttaatg tttatagttg ttgggatagt aataccaggt
     3241 gcgtttaaag atagtaatga gtttattgga acgtatgcaa taaagcaagg tctttctttt
     3301 ggagcaggtc ttataataat gcttcatggg gtaagaatgc taataaatca aataatgcca
     3361 gcatttcaag gtatatctga aaaagttgtg ccaaatgcac tacctgcttt tgactgtcca
     3421 atattattta attataaacc aaatgctgta ttaataggat tcttagttgc tatgctaaca
     3481 tctacagcta tgattttaat atgtaactac tttaatgtat ttaatttgct acttatacca
     3541 cttgttataa cttcattctt tgagtgtgga gcagcagcag taataggaga atcacaaggt
     3601 ggtatacgtg gagctataat aggtacagct atagctgcaa tatttatggt agttttagta
     3661 ggaatatctg ctgtaattta ctcaggaact attcaaaatt ggatactaat atttggaggt
     3721 aacgaccttt cattatttgg tataattagt aagtttatag gttcactatt tggaggaata
     3781 ttatagatat gaaatttaaa tacatagatg agataagaag attaattgat ataatagaaa
     3841 acgaagaaaa agtcaatatg gaaaaagcag tacaaatcct tgttgaagca atagaaagta
     3901 aaaatgctat attttcattt ggagcttcac atgcaggaat attaacagaa gaattattct
     3961 atagagcagg tggctgtgtt cttataaatc cagtatttgc aaaatctcta atgttagata
     4021 cagcgcctat aactcacaca agtaagatgg aaagattaga aggatatggt acagctctag
     4081 ctgataaaac tccaattaaa aaaggagatg tagttatagt tcattcagta tctggtagaa
     4141 atccagtaag tatagagttt gctatgaaag ctaaggaaaa taaggcaata gtaatatgta
     4201 taactaattt aagttactca aaaagtgtat cttcaagaca ttcttcagga aaaaaacttt
     4261 atgaagtatg cgatttagtg atagataatc atggtcaaaa gggagatgcc tgtgtagaga
     4321 tagatggatt aaaccaaaaa gttagtccaa cttctactgt aatagcaact actgtgatga
     4381 actctattat agctcaagct actcaggaat tagttaataa agggctaaag aatcctccga
     4441 ttttctatag tgccaatatt gatggtggag atgagttaaa taagaagatt tttgatgaat
     4501 ataaaagtgt aattcattat gaatattaag gttaaaaaaa tatgttaatg aactttgaag
     4561 tttatcaaca aagaatatta ataatatata ataaatgcta tcatttaaaa gctgtaggta
     4621 aaaatctaca gctttttatt attgtagatt aaaaagatgc gtaattaaat tttaaattgg
     4681 agaaaataaa ttggaaggta tcaattataa atattagaaa cgttgtcata gaaaaaaact
     4741 atattaaaaa aaagtacata gaaaaaactg attagacaat aaatacatgc tttgataata
     4801 ttttaaatgg agtgaaaatg tgtagaatta taatgaaaaa agaaaataaa aatgaaaaaa
     4861 gaaaataaaa tggagggaat aaaagtgggt atgttaaaaa aattatgttc actagctatg
     4921 gtagcaatta tgactacaac acttataaca gggtgttcaa gtggaaatgg aaaagattca
     4981 aaaaaggatg gcgaaaaaga aaaactagtt ctttggatgc caccagtaga aaataatatg
     5041 aaggaagttt gggaccctat tttagataaa tttgaggaaa aaaataactg tgaagtagat
     5101 ttacagataa ttccttggga taattattct gaaaaatttg caacagctat aagtgctggc
     5161 gaaggtccag atgtaggata tatgtatgca gaaatgtatc cacagtttat agaaagtgga
     5221 gcagtagaag atttgactaa ctatgcaact aaagaggata aagagcaatc tatttatata
     5281 aaaacatctg aaatgatggg tggaatgtat ggtatgccat tccaagcagc aaatccaggg
     5341 gtattatact ataacaagga tattttagag aaattaggag agaaacctcc taagacatgg
     5401 gaagatttta aaagaatttg taaaaaagca acacaagata ctgatggtga tggaaagata
     5461 gaccaatggg gattagctca gggatgggga gctaaaactt ttggtaactt aaactggaac
     5521 tggtatcctt atttatggca agctggtgga gatattttta atgatgattt aaaatcggtt
     5581 aaatttaatg ataagagtgg tttagaagca gctaatttct taaaagaatt acaagcatat
     5641 gtgcctgaag attctttatc taaagatagt aatgaaatga tagaaagcgt atttggacca
     5701 gggaaagcag catttacaat aatgttatct tctgctgcaa caagtgtatt tgatgaatca
     5761 ttcccggatt taaattgggg gtttgtaact ggactagaag ataagaaagc agcaactttt
     5821 ggagcagtag accatttatc tttaatgtca tctgctaaaa ataaagaact tgcatacaag
     5881 ttaatacaac atatgttaag tgttgaatca atgacagaat ttcataaagc aataccaaga
     5941 gcaccaataa caaaaggtga accatatcaa ggtgatgaaa gattcaaaga aatggtggaa
     6001 aatgacaaaa atgtatatag accattagta gtaggacctc atggagttga aatatatgaa
     6061 tatttatgga aagaacttca aactatgatt tcaggagata aaactccaaa acaagcatta
     6121 gatgatgctg caaaatattc aaatgattta ttagcacaat aaaagtttaa agctgtaata
     6181 tgttgtaaag gactttacag attaaaatta gcattgtatt tataaataca atgctaatat
     6241 ttatattgga gaggataaaa tggaaaactt agttagagag tttgaagaaa attcaaaaag
     6301 aaaaaaaaca aaattaaata ttaaaccata tatgtatata ttgccattgg gaataatttt
     6361 agtggcgttt tatgtgatac caataataat gtcaatatac tttagtttta caaagtataa
     6421 tattattagt ccagccactt ttataggttt agaaaattat aaaaaattat ttacagatga
     6481 aacattaaaa gtatctatta taaatactat taaatttact gttgttgtag taccttgcca
     6541 aactatatta tcattgattt tggcagtttg gattacagga aaaggaaata gtaaaatagc
     6601 ttcatttgct aaaggtgcta tatttatacc agtattatca tctatggttt tgataggtat
     6661 ggtatggagg gctttattga atggagaagg ttctattata tatcaggtat taggtgcctt
     6721 tggaatagaa tcatctaaac ttttaggaga tagtaagaca gcattaccta cacttatgtt
     6781 tatatctatg tggaaaaata tagggtattt catggtaatt tatatttcag ctataatgaa
     6841 tttaccaaaa cactgctatg aagtagcgaa ggttgatggg gctacaaaac ttcaagaatt
     6901 tataaaaata actgtacctt tattgaaacc aactacaata atggttgtat ttttagggtc
     6961 gatatggtca ttacaagtat ttgacttaat ttatactgtg acaggtggag gtccaggtat
     7021 atctactatg agtatagtta tgcatgcatt taacttaaac tttaaaaact ttaattctgg
     7081 atatgcaatg actgtagcaa acgtattatt tttgttaata gcagtagttt cgattttaca
     7141 aaataagctt gtaaaacgag acaattcaga tttttagaaa ggtaggtata atatgaaaac
     7201 agcaagaaga ttattaagtg atggtttact tttatttata gcgtgtgcaa gtttggtacc
     7261 ttttatatac atgttgataa tatcattgaa gataacatat aactcatata gtttggatat
     7321 atctttttca acagtaactc ttcaaaacta tatagatatc tttactaaaa aaggatttgc
     7381 acaatatttt tttaatacag caatagtatc tttttcaggt gtattattaa atttagtatt
     7441 tagtacatta gcaggatatt cttttgcgaa gatggatttt aaaggtagtg ataagttatt
     7501 tttatttatg ataatgactt taattatacc ttcccaagtt acaatgatac cactatatat
     7561 aataatgaaa cacttaggat ggataaatag ttatcttgca cttattatgc caataccaac
     7621 tgcatttggc gtatttataa tgagacaagc tattttagga gtaccaaaag agttattaga
     7681 atcagcaaag atagatggtt gctcggattt tagaatttta atacaaatag tattgccttt
     7741 aatcaaacca gcattaatta cactagcaat atttactttt atgggagcat ggaacgagtt
     7801 tatgtggcct cttatagcta caactaaaga tgcaatgaga acattaacag ttggattatc
     7861 tactcttaag acattccaga taactaatta tggacagatg atggcagggg ctacaataac
     7921 atttttacct ccatttattt tctatttgat tttacaaagt aaatttgttg aaggtgtgtc
     7981 tttgtctggt ataaaaggtt aaatagtcta atttaataac ataaaagaaa cttatataaa
     8041 tatggaggaa tttaaactat ggaaatttca tatgtactga aagattgcac attcaaacac
     8101 aataaatact cagttataga agctgaaact tggaaagaga aggatttgga tattgtagta
     8161 gctaaaggtg aaaaatttgc atttcaaatt atgctaaaag caacagaaga atttaattgt
     8221 acaattgata aaagcagtac tatatcatgg aaaggactgg aaaatagagt aagattagca
     8281 ttaaatgtgc ctaatagttt agaaaataat tttagtataa atatattagg atatgttcaa
     8341 gatgatacaa aggcatttgt gaatgatgct attttaaggg ataaagatgt gttagtagag
     8401 caatatttgc ctcaaacatt ttggattgaa ggtcaagtac cagaagattt tgaagaaaat
     8461 aaccttaata taggtattga tatatttaaa agctttggat atgaagatga agaaaaagtt
     8521 tgtacaattg atgttcctgt aaaagtaaga aatgtcgtgc taaagccttt agataaaagc
     8581 aagttctttt tagatttatg gcaacatcca agttgtttag ctagaatgta taaagttgag
     8641 ttatggtctg atatacattt tgaaatagtc gataactatt taaaagaatt agcatcttta
     8701 ggagaaaaag ttgcaacagt tatagtttca gattatccat gggcagggca aagttgttat
     8761 aaagtttata aaaatccatc aaacctatat gaatataaca tggtaagtgt atcaaaagga
     8821 ttggatggaa agattaaatg taactttgag agtatggata ggtatataag catagctgat
     8881 aagtataaaa tgtctaagga gatagattta tttggtctat taggtaattg gtgtgctggt
     8941 gagtttggaa atcctgtaga aggttataag gaccctataa gagtaagata ttttgatgaa
     9001 aatgataaag tatttaaatt tataaacaat acaaatgatt taaaagaata tataggatta
     9061 gtattaaatc atttaataga gtgtggatta tgggatagag taaggattat agctgatgaa
     9121 ccaaataatc cagaagtagt caaagagtgt atagaattta taaattctac tgtaggtact
     9181 catcaagtta aatataaatc agcaactcac gaccaaaact ttttagatag agctaaagat
     9241 gaaattgatg atatgtcaat aaatttaaaa cttactatac aaaattataa agatatagaa
     9301 aatcttaaga aaaaaatcaa tgacaagggt gggatattga cttggtttgt atgctgtttt
     9361 ccagagaaac caaatagttt tttaagttct ccttttgtag aaaatagaat aataggatgg
     9421 tatacttatt actttggtct tgatggattt ttaagatggg actataattt atggacagaa
     9481 gacccttgga aagattcaag ttacaaattc cctatttgga aagctggaga tatgttcttt
     9541 gtttatcctg gaaaagattt aaagccagtt agaagtgtca gaatggagaa tttaagattt
     9601 ggaatacagg actttgagtt atttacaatg ttagaaaaag aaaagggcag agagtatata
     9661 gaagttgagc ttatgcaaga attattaaat aaaaaagaaa atgctgaaat taaaggtttt
     9721 ggtgacatag aattaggata ttcacttgat aattggggat atgataaagt taaaaaatat
     9781 gtattagagt tgttagacag agtataaaaa aatataagag tgtctcaaaa tgaacttttt
     9841 agttcatgag gcactctttt tgtatgaaat caatatagtt tcattgtttc aacaatgaaa
     9901 aataggatta cttatattct aagatagccc tttagttttt tagttgtatt caatgtttta
     9961 aaactaacta taaacgctat aaagtttata aatgacaata attatatgta acatgatgta
    10021 ctttaatttg ctataatagg gattgaataa tattatatgg aagataacat tgtttggggg
    10081 gattaatata aaaagaatag gtattattat aatgtttttt ttaagtatag cttttattgg
    10141 attttatctt gcaataagag gtaacagtga tacagttaag atgcaacgag ggataaatat
    10201 aggtaatgca ctagaatcac ctaaggattt tccgtgggat gtaaaaatgt caaataagtt
    10261 ttttgatgat ataaaagatg caggttttga cacagtacgt ataccagtaa ggttttctga
    10321 ttatacgtca gattctgata attttaaaat tgatgaagaa ttttttaaaa agattgataa
    10381 atatgtagat tatgcattgg ataaagactt aatagttgtg ttggatttac atcattttga
    10441 agaaattatg aaagaaccaa gagttcataa agaagagttt ttaaaaatat ggcaacagat
    10501 agctaatagg tatcaaaaat atgataaaaa attggtattt gaattattaa atgaacctaa
    10561 agaaaattta tcttcacaat tactaaacga atacattgaa gaagcaataa agataattag
    10621 aaaaactaac cctaaaagaa ctattatagt gggaccatat aatttctatc aaattgatta
    10681 tttgaatgaa ttaaatgttc caaaagattc taatatagta gtttcttttc actattacga
    10741 accaaatgat tttgcatttc aaggaaacat ttatcataat ggatttgagc acttgagcaa
    10801 tattacttgg gaaggaacaa atgaacaaat ggattattta aaaaaaaggt ttgatattgt
    10861 agaaaattgg gcaaataaaa ataaggtaaa aatattcttg ggtgaatttg gagtaacaaa
    10921 agaagcacca gaagcttcaa gaagagcttg gataaaagct gtaagagaag aagctgaaaa
    10981 acgaaacttt tcatgggctt attgggagct tgcttctgga tttggtatat acaatcaaat
    11041 tgaagggact tgggatagag acatattaag tgcattaata gaaaagaggt gattttatga
    11101 agaaaaattc aaaaaagata cttataactt taactattat tactaatatc gtatatatat
    11161 tgtggagaat tttttacaca gtacctaaag aagaagggat gtttgcctta atctgtgcta
    11221 taatacttct ttttgtagag ataatgggta tgatggaaat gtttgtacat tattatggta
    11281 tgtcaaatat tgagtatcct gaaaaaccag tcataagtga agaactttat ccacatgtag
    11341 atgtattcat tgcaacatat aatgagtctg ttgatttggt gagaaaaact gtaaatggat
    11401 gtattcatat gcagtatcca gataagaaaa aggtacatat atacatatgt gatgatggaa
    11461 atcgtgaaga aatgcgtatt ctagcagaaa aaatgggtgt aaattacata actagaactg
    11521 aaagagaagg agcaaaagct gggaatttaa ataatgcaat gcaacataca aactctcctt
    11581 tgattgctac gtttgatgca gatatgatac caatgcatga ctttttaatt gctacagtac
    11641 catacttttt gaaaaatgag caagctaaaa aagatggaga aaaagaggag tatgaaaagg
    11701 ttggttttgt gcaaactcca cagagttttt ataatccaga cttgttccaa tttaatctgc
    11761 actctgaggg aaggattcca aatgagcaag attattttta cagagatatt caattggcta
    11821 gaaataggac aaattctgta atttatggtg gtagtaatac agttatttca agggaagcac
    11881 ttgaagaagt tgatggattt tatacttatt ctattacaga agattttgca acaggtatct
    11941 taattcaaag taaggggtat agatgttatg ctattccaga agtacatgca tcaggtttat
    12001 ctcctacaga tttaaaaagt cttattaagc aacgtgaaag atgggcacgt ggatgtatac
    12061 aaactggtag acgtctaaat attttgttta ggagaggttt aggtttttgg cagaaaataa
    12121 gttatatttc ttcaattact tattggtatg caagtataaa acgctttgta tatataatgg
    12181 cacctatttt attttctgtg tttaatgtga ttgtagtaaa atgtacatta cttcaagtat
    12241 tggtattttg gttgccaatg tatattttaa gtagtttatc tcttaaaata ttttctcaaa
    12301 atataagaaa tactagatgg accaatatat atgaaactat aatgtttcag tctttaatgc
    12361 cagcagtaat acttgaaacg tttgcaatat ctaaaaataa attttcagta acaaataaga
    12421 ataaattaga agaaaataga atgtataagt ttttacaggg aataccttat tttatttaca
    12481 tggtcttatc tatcatagga attttaaaaa tgtttgtagc aatatttaaa atgagttcaa
    12541 tgacatattc agttgtatta ttttggttga ttggaaacct ttttaacctt gttatggcta
    12601 cattatttat atcagggaga caacagttaa gaaagtctga gcgttatatt gctgaaattg
    12661 attttaagtt aaagcaaaac tcttatgttc tttcttcaaa aacgattgat atatcagaaa
    12721 atgggtttgc atttttgcta gaaaatccag aatatatttc cccagaagaa gagttcgagg
    12781 tagagtttag agaaaaatca ggaaatgaaa tgtatatagc taatatgaaa gcaaagattg
    12841 ttaatgttgt ggaagtaaat tcaaagtgga aatatgcagc ttatattaca catattgaag
    12901 atagtgaaat agataattgg atgtgtattg ttcacgatag aatacctact ttaccgatga
    12961 caatatctaa tcaactaggt ttctttgatg atttgcaaat taatgtaaaa aaacgtattg
    13021 aaaagattag aacattatca cgaagaagtc caagaatcaa tatgaatttt cagatggata
    13081 taaaaaatac aggtaaatta agaatagtaa attttaatta tcaatatgta ttattaaatt
    13141 ttgagaataa aaatatttat cctaaagaaa tagcattgga aattaatgaa aatattgtgt
    13201 tagaatgtga tttatgtgag ggtaaaatcg atgaaagagg tctcttatat aaagtaaata
    13261 acattgacag cataatgcaa aatttctttt taagagatga aatgatggat tggatattac
    13321 aaaataagac aattcctgtt tcaaatccta gtgaaaagaa ggaaaaaagt atagatgagt
    13381 ttgaacccat ggaatatatt taggagagat gaaatgaaga ctttaaaagt gtttttttgt
    13441 tctgcaaaat atcttatttt ggtgtttatt ttttttataa atactacttt tataagttca
    13501 gcgacaagtg ataaaatttt agatttatcc tttaaaaaga tagagactga tttaagttct
    13561 aggattacat atgaagatac aggtgtaaaa atagaaacag atagttctaa aagtgataag
    13621 gaacgttatt tatatattta ccaaaatata aaagaaaatt ggagtatgta taataatttt
    13681 tatattgaaa ttcaaaataa aaataaaagt tcccagaaaa taaatttaag tatacaaagt
    13741 aagaatatgc ttgaatttag gttaaaagaa ggtagtgaaa tatttttgga agataaaaat
    13801 ataatatatt cagataaaat aaaagaagga tgcatagaag taccaggtga atttgaaggc
    13861 aaaatttatg tgaactttaa tagccttata aatgagaaaa gtaatgttgt gctggactca
    13921 agtatgcttt caaacattgt tagttgggga attacattta ttccatctga tgaagaacat
    13981 aatattgtta ttataaaaaa gatatcttta ttgtctgaag agaaattaag ttttttacat
    14041 aatattaaaa taactggcaa tgaagaagta caaataccag tattgggtca aagtataagt
    14101 cagtatgagg tattaggtct taaacgtgat tctaaaatta agtattctct tatgggaaaa
    14161 caagataatg tgagcatatc tcaaaatggt aaattaacat taagtaataa agccgaacct
    14221 ggacaaatta ttttacaagt taatgttgat gataagttta aaattggaag gaaaatagca
    14281 ttaactgaat cttggagtat taataaaaaa gataaggatg gtgttccata tacattggta
    14341 tctcctgagc aatcaccaac agtacaatat atgaaaaaaa taaactttat gaataacatc
    14401 ataacgtttg ttcgaatttt atttgtatct ctagtaataa tatgctttgg aatatattta
    14461 tattggaaaa aatgttcaaa aactaagtaa agagaggagg ttagattgtg ttattttcaa
    14521 gtttaatttt tttattttat tttttaccta taactttggt tttatattat gtatttaggt
    14581 ttaaccgaac gatacagaac atgatattgc ttgctgttag tttatttttt tatgcttggg
    14641 gagagccaaa atttgttgtt atcatgatag tttccataat aatgaactat atatttggtt
    14701 tactagtaga tagatatagg gaaagtaaaa taaaagtaaa agttttttta gttttgatgt
    14761 gtgtatataa tataggtgtt ttatttatat ttaaatatct agcttttgcg ctaagaaata
    14821 ttagtgaact tattaatact gaactaacta tacctagtat tgttttacct ataggaattt
    14881 cattttttac atttcaggga atgtcttatg ttatagatgt atatcgtaga catggagaag
    14941 tccaaaagaa tcctttttat gtgggactgt atatagcatt tttcccacaa ttaatagcag
    15001 gtcctatagt tagatatgag agtgtggcag aacaaattct caatagaaaa gaaacttgga
    15061 ataaattttc tattgggact tgcaggttta taacaggtct aggcaaaaaa gtattaattt
    15121 caaataacat ggctatagta gcagactata tttacactat gaatagtcaa ggagaaatag
    15181 ctgcatcact tgcatggctt ggttcaatag cctatactat gcaaatattc tttgattttt
    15241 ctgcttattc agatatggct ataggtttag gtcttatgtt tggttttaaa tttgaagaga
    15301 actttaatta tccatatata tcaaaatcaa ttagtgagtt ttggagaaga tggcacattt
    15361 ctttgggaat gtggtttaag agctatatat actttccgct tggaggttct agagtagcaa
    15421 ataaagatat tatgattaga aatatgttta tagtgtggtt atttactgga atatggcatg
    15481 gagcagaatg gacatttgta atttggggga ttttaaactt tgttttcttg attatagagc
    15541 gatttataat gtttgaaaag attgaaaatc acaattttat aaaacatatt tatgcgttat
    15601 tagtagttaa ctttgggtgg gtattgttta ggtcacctaa tttaaaagaa gcatataatt
    15661 atttcaaagc tatgcttgga ggcaatggag ttatatggag tgattataca tatatgtttt
    15721 taaaagaata ctgggttttc tttatatttg catttatttt tagtgtacct attgcaaaga
    15781 aaatcaataa gtttgttgtt gaacaagcta aatatagtat gatatttaac gtattttatc
    15841 caatctcagc tattttattg ttctttattt ctgtgacata tttagtaact ggaagctata
    15901 atccatttat ttattttaat ttttagttgg gaggaaaagt atgaatagat tttttaaagt
    15961 aagattaata actgcaatta tgttcttaat tattttattc gctttctcta tctttaatat
    16021 ttgttattct ggaaaagata tgatacagga gataaaaaat tcagttgctg aaaaaagtag
    16081 tttaaaagat acaatatcat ctattgataa cacagtaaat gacaaagtaa tatataaaaa
    16141 tacgttcatt gaaacttatg gctacttaca aaagttaatg ggtaaaaatg agttttcaaa
    16201 atttagtgtt gtaaaagata cttctggaaa gatgcattat acatattttg ctaaagctcc
    16261 aaatccagtt ggtgtgatag cagatagggt aagaaaattt tctgaggaaa tggaaaagca
    16321 aaacactaaa ttagtttact taatgactcc agataagtat ataaaaggag tgacaaaatt
    16381 tccaaaagga ataccttatt cttatagcaa tgagacagca gataattttt taaatcaact
    16441 aaaaaagtat aatgtggact atattgattt tagagaaaat attttaaaga gtggtattcc
    16501 aaaagatgat ttatttttta aaacagacca tcactggaaa gtagaaactt ctttttgggc
    16561 atttggagaa ttagttgagc aacttaataa aaaatacaat atgaatttag atgaaaatca
    16621 ttactataga gataaagaaa attataactc aattgtatat cctaagtcat ttcttggttc
    16681 tatgggaaga aagactggta tcttatatgg tggtatagat gattttacat taatttatcc
    16741 taaatttaag actaattata cttattatac agattctaaa tctcaaaaat ttgaattaaa
    16801 aggaaggttt gaagagagtt taatattatc atatcctttt aatgcagacc ttgatttaat
    16861 ggatggtcaa tcagataagt attttacata tttattgggg aatagaccat tagttaaaat
    16921 aaaaaatata gaaaatccag atggattaaa agtgctgttt gttaaatatt ctctaattgt
    16981 accaacagca gcattttttt cctctgtttg ttcatctata gatatgatag accctagata
    17041 ttatgatgga gatatactag aatatgcaaa atcccatgac tatgactttg tttttatatc
    17101 tgtatatccc caaaatctta caaaagaatt ttttccgttt tgtaagtaaa tttctaaaga
    17161 aattttctta taaaataaaa aataaatggc taactagtta aaagttagcc atttatctta
    17221 agtgttactc taagaaatgt tttttttacc aaatacttct ttatctattt ttccaagtga
    17281 ttttcctaca gtaattgatg ataccatagc accattaaca tttatcatgg tacgtcccat
    17341 atctagtata gggtctattg caagtatacc accaactaat ggaaaataag aaccaagacc
    17401 aactccagag ataacaactg aaacggccat tgtagcagtt cctggaagac cagctattcc
    17461 aaacgaacta attgttataa caattaacaa cataacataa aggctcatat ccatgtttac
    17521 tccagccata ttggcaagtg ttacagccat taaagcagga tatatacctg cacaaccatt
    17581 catacccata tttgcaccta aacttgctgt aaagcttgat acaccctcat caactccgtg
    17641 attttgtact agactttcta tcgttacagg caatgttcct aaactagaac gtgaagtaaa
    17701 tgccagaagt aaagcttcag ttgaatttct tatataagta atgggattta gaccatttaa
    17761 agttataaaa attaaatgta tggtaaataa tattgccact gatatataaa gagctaatat
    17821 aaactgtaaa acagatacta ttgagctaat accttgtgaa gttattgtat ttgcaagcag
    17881 agcaaccaca gcataaggca taaatttaat aacagtcata gacacactta caattatttt
    17941 gtaagatgct tctataaaat ctataaatgg ttttattgtt tcaaagtgtt ttttacttaa
    18001 tctctttatt gaggtagcta taaatgtagc aaatattatt gtagctacaa tatttccatt
    18061 tgccatagcc attacaggat tagatggtag taatcctctt aaagtatcca caagagagtt
    18121 tatttcttta agttcttgat tttcaactaa agtaggcata ttagctccta cacctaactt
    18181 cattatattt gcaacgataa ttccaacaat tgcagctata gcagtagtag caagaagcat
    18241 acctaaagat ttaaatgtca gagttccaag attgttatct ttcatattta taattacttt
    18301 tataatggat aagaaaacta aaggtactac taacattttt aataagtcca taaatccaga
    18361 accaaatagg ctataccact tgcttacttc ttgtagccaa gtgacttgag tggtatcatt
    18421 aggaaaacca gcaaaagctt gtattatgat tcccaatatg agtcctagta ctgtagcata
    18481 tattgttctc aatgaaaatt ttattttttt cttttcaaat tgtctaacaa taaagaatgt
    18541 acctagaagt aatattatga aaaaaatcgt tttaaaatct gtaatcataa gaaattgtga
    18601 taaaaatgaa ctttccaata ttttttctcc ttttatttaa aaataaattt ttctaagttt
    18661 aaatgatacc aatatattaa aaaaatgtca atattatatc acaatttact taaatagagt
    18721 aaaataaatt gattttgaaa aatgtgatta agtatagaaa aatagggtat aataaatgtt
    18781 taaagaattt gaattgtggt aagaaaataa aaaatagctt ttataatatt tgatttcaaa
    18841 ttaaaatatg ttaaataaat aactaaaaat tgttatgaaa aatgtttaca tagaatatat
    18901 atcaaatgaa aagcttaaaa taagtgaaga aattaactag acatcaaagg agagataaaa
    18961 tgagagtagt aataattggt ggtgttgcag ctggtatgag tgctgcagca aaattaaaaa
    19021 gaataaaacc tgagtatgaa gttgttgttt atgaaaaaac tgaaatagta tcttttggtg
    19081 cttgtggatt accatacttt gttggtggtt tttttgatga tgctgatgaa ttattagcaa
    19141 gaacaccaga gaaacttaga gaagctggaa ttgatttaaa tatatttaga gaagtcgttg
    19201 aggttgatag tgaatctaag aaaataaaag taaaaaatat aaaaacagat gaaatatatg
    19261 aagattacta tgataaattg atgatagcaa ctggagcaag aagtattatg cctcctataa
    19321 aaaacataaa attgaagaat gtttctacat taaaaagttt atatgatgga gaatatctaa
    19381 aaaaactttt aagtaatgaa gataataaaa gagtgacaat aataggagct ggttttatag
    19441 ggttagaagc agttgaggca tgtaagaaat taggtaaaga tgtagatgta attcagttag
    19501 aagatagaat attgccacaa gtatttgata aggagatgac ggatgtatta gaagaagaaa
    19561 taaatagaca taatgttaat ttgcacttgg atgaaatggt tgtagaactt tgtggagaag
    19621 ataaagtaga aaaagttata acaaacaaag gggaaataga tacagatgta gtaataattg
    19681 ctactggtgt gagacctaat accgagtttt tgtcaaatac taatataaaa atgctaaaaa
    19741 atggagctat agtagttgat gaatatggaa gaacttctgt agaagatata tactctgctg
    19801 gagattgtgc aactataaag aatatagtaa gtaatgaaaa tgtatatgtt cctttagcta
    19861 ctggagctaa taaattaggt aggatagtag gagaaaacct agcaggaaga gaagtatctt
    19921 atcaaggttc actatcttca agttgtataa aaattatgga tatggaggca gcatcaactg
    19981 gaattacaga aaagcaagct aaagatttag gcataaatgt caaatctaaa tttatttctg
    20041 attataatca aactcattat tatccaggaa gaaataaaat atatgtaaaa cttatttatg
    20101 atgcagatac aaaagttatt ttaggcggac aagtggctgg gtttaaggat gctgtccaaa
    20161 gagcaaatgt attggcagct gctatctttg gaaaaatgac cacaagtcaa cttggaatgt
    20221 tagatttatg ttatgcccca ccatttgcaa gaacatggga tgttttaaat gtagctggaa
    20281 atgtgtctaa ataatattta gattatagct aatatagttg acaagcaatt aagggaatga
    20341 atttacctaa actattttta tcatggtaaa attcatccct aaatttttat tccaaatttt
    20401 tagaaataag gattccatta aattattcat caaaatttaa acgcaagctt aatctatctt
    20461 ctccaagaat agtattttca aaaacattag tagcattttc taaatacata ctaggtatgt
    20521 ctaaagaagg actaaaatgg gtcagtaaaa gttgtttgac atttccaagt tttgcaagat
    20581 tagcagcttc tctaaaagtc atatgtttgt ttctaacagc ttttgatata tctaaatcat
    20641 caccatacat ggcttcacat ataaataaat cactatcttt tatgaaatct ggtattgatt
    20701 ctataggtct tgtatcggtt gtaagactta tcttaactcc ctttctcaat tctcctaaga
    20761 ccatatctgg agtgtattgt tttgaattta aaactatatt ttgaccttct tggagtttgt
    20821 tccataatat tttaggaact tcgtttttag ttgccttatc tatatcaaat ttagggtttc
    20881 ttctaaaatt aaaactgtat cctaaacact ctatagagtg ttctaaactg attgttgaaa
    20941 tttctaaatt tcttaaaact ttattatcta aagaaaaatt gccttgaggg ttttctataa
    21001 tttttaaagt gtatggtaca tattctacta aatttctcat agaacgtata caatcgacta
    21061 ttccaactgg accaactata gttaaatcac ttgtacgacc actattccca attgttgata
    21121 aaagtcctaa aagtccaaat atatggtcac catgtaaatg ggttatacaa atcaagtcta
    21181 tatctttaaa gccacatttt tttagcttca tagatacctg agtaccttca ccacagtcaa
    21241 ttagtatttt tctaccttta tagtttataa atacagagga tagaaatctg tttggcatag
    21301 gtacatttcc accacatcca agtaaagtta aatctatcat tttttcccct ttctaaaatt
    21361 aacacaaatc tgttgtttgt ttttattatg atatcctatt ataattattt taacattaaa
    21421 tataacaata aatgtgtatt tgagtcaaga aatattatca aataaagttt ttatatagca
    21481 atgtataaaa atttggagaa cgaaaagtga aaattatatt taggagtatt taaaggagga
    21541 aaatttaatg aaagtattaa agaaaattac tattttgtca ttgattacat taaccttagt
    21601 tggttgtagt aacggagaag ataaaaaagt ggttgaaggt tctaagaaaa ttcaaaattc
    21661 taaaaaagat gaacagccta taatagtaca taaagatgca aatatattga aagataggta
    21721 tgagccacca aaaggattta aaagagttga tgtagaaaaa ggtagttttg aatcatttct
    21781 taggaatact aagctaaaaa attatggaga gaaagtaaag tattatgatg gaagaattaa
    21841 ggaatcaaaa ggtatttatg atagtgtatt tgatgttgat attggcaata gagatttgca
    21901 tcaatgtgca gatgcgatta tgcttatgag agcagaatac ttgtatcaga ataaactata
    21961 tgatgaaata agttttgatt ttgttgatgg ttttaaggcg aagtattcaa aatgggcaca
    22021 aggatataga atttcagtaa aagatagtgg ttcaagctgg tataaagcaa ctgaatattc
    22081 aacatcttat gagagtttca gaaagtttat ggatatagtg tttgcatatt caggaacgtt
    22141 atctcttgaa aaagaattgg agcctgtaaa agttgaggat atgaaaatag gagatgtgtt
    22201 tatagttggt ggaagtccag gacatgcaag tataattatg gatatggcag aaaatgaaaa
    22261 gactggtaaa aaggtattta tgatagccca aagctatatg cctgcacaac aaacgcaact
    22321 tcttattaat cgtaaaaatc caaatttgag tccttggtat agtttagatt ttgaaggtga
    22381 tttgacaata cctcaatgga cttttaaaag agaccagttg aaaagatttt aaaataataa
    22441 ttttatataa taaaagaaaa atatagatgt gaaaaaatgt aattagatta aaactaattg
    22501 gatatggaca aaattaaatt gatagataaa tattaaaaat tacactaatc aatcttacta
    22561 tagtttaata tttatctaaa aagttttatg taaaatataa attttatatt aaagctaaaa
    22621 ctaatttgag gaggctaatt tatattgaag attagaaggt atttaatttt ggtaattata
    22681 attctaatta caacaagtca gagttttatt tcttatgggc tagagcgatt tgaagaaata
    22741 accatttttc acactaatga tatacatgga agatatgctg aaggtgatga ccatatacaa
    22801 ataggaaatc tagccacatt gaaaaaagaa actccaaact caattttagt agatgcagga
    22861 gattgtttgc atggacttcc aatagtaaat atggatagag ggaaaagtgc aattgaatta
    22921 attaaatgtg ctggatatga ctatattact cctggaaatc atgactttaa ttatggaaaa
    22981 gatagactgt ttgagttatc taaaagtgct aatttaggag agaatacttt aaaattttta
    23041 tcatcaaata tttttgaaaa tggaaaaaaa gtttttgata gtaatgatat aaaagaaata
    23101 gatggaataa aaataggatt ttttggactc tcaacccagg aaacaaagaa caaaactgga
    23161 tttaaaaatg tggaaggatt agagtttaaa aatcctatag aaagtgcaac agaacaagta
    23221 aatgaattaa aaggaaaagg tgccaatgta atagttgcaa tatgtcatat tggtacaaat
    23281 agtttaagta agccaaatag tatagatata gcaaatgagg ttcaaggaat tgatttgatt
    23341 attgatggac acagccacac taagtttgaa aatggtaaac aagtgggtga tacattgatt
    23401 gtaagtgcag gacaatattt agaaaatatt ggcgagataa aactaacttt ggatactaat
    23461 gatgtagaaa atataaaaat agaaaataaa tcagttagat taattggaaa agaagaagca
    23521 ttaaagtata agcttgacta cgttgtaagt aaaaaggttg atgaaataaa agaacaacaa
    23581 gaaaaaatat tagataaagt tataggtgca actacaaata ctctagatgg ttcatatgaa
    23641 aatgtaagga caaaagaaac taatttgggt aatttaataa gtgacatttt actagataaa
    23701 actaaggctg atatttcttt atttaatgga gggaatattc gagatacgat agaaaaaggc
    23761 aatataacaa gaagagatat tgtagatgta tttcctttta gcaatactat agttaccaaa
    23821 gaacttacag gagcacagat taaagatgta ttggagcatg gagtaaaact ttatccagaa
    23881 aaaagttcag cctttttgca agtgggagga atttcatact actttaatcc taaacaaaaa
    23941 gaaggcgaaa gaataacaag tattcaaaaa gaaggaaagc cattagattt aaataaaaag
    24001 tatgtagttg caacgaatga ttatatagcc tcaggtggag atgaatttcc ttgtttttca
    24061 aaagagccaa tactaaagga atttggaaat ttagagagtg cagtcattga atatatagag
    24121 tacaaaaaag aaatatcaaa aaatgtagat ggaagaattg gtattaaatc taaagatgag
    24181 ttaataagca agaatcatga gagtgatttg atattaagtg aaaatgaaaa aaatatcaat
    24241 gataatgaag agaatctatc taataatata aaaagctctg atagtgacaa aaataaaagc
    24301 ttagataaaa caagtgctag agaaagttct aatcaaggaa tatctaaaag agaagctaaa
    24361 gaaaaatctc ctaaaacagg agatttaggt ttttcaaata gtataattat atttatagtt
    24421 tcaagtacat tgatatgttt attgaatttt aatcaaaaag agttaaagga taaaaagtca
    24481 aaataaaaat taatgagcaa aattagtaga tagtaattta attttagata atagtaaaca
    24541 gcaaaggaaa ttagttatat aaatactaat ttcctttgtt ttatgcttta acatgattat
    24601 ttttaaataa aattgtattt tatgaaaaat aattacacta aatggcatag tattatgggg
    24661 atttttagtt tttattcaag taaatgaaat catattaaca acttctgata tatttacaaa
    24721 ttgataaaaa aatgatttta ttagtatgat ataatttagc tagtatatat gatgatggag
    24781 gatataggga tgaataatat attacttttg gaagatgaca aatctttaaa tagaggaatt
    24841 agttttaaat taaaaaaaga aggatacaat gtttttagtg catttggtat agaagaagca
    24901 aaaaatattt tcgcaaaaga agagatatgc ttaattattt ccgatatagg tctacctgat
    24961 ggtagtggtt ttgacttttg tgaggagatt agaaagaaaa gtgatgttta cataataatg
    25021 ttgacagctc ttgatgaaga ggtggatatt gtcacaggat atgatttagg tgcagatgat
    25081 tatatcacaa agccttttag cttgatggta ttaatatcaa aagtgaatgc gcttatgaaa
    25141 agagtaaata cagtaaaaaa ttatactcta ttagtttgtg atgatttatt cttttattat
    25201 atagaaaata aattaattat aagaacagat gataaggaag aagagataat tttaagtaag
    25261 acagagacaa aactattaaa atatttgatg gaaaattcta tgcagacatt gactaaagaa
    25321 caactgttag aatctttatg ggatagtagt ggtaattttg tagatgataa tacgattgca
    25381 gtaaatataa gaagacttag acaaaaggtt gaaaaaaatc catctgaacc taaatatata
    25441 aaaacagtaa gaggggttgg atatatgtgg ggagaaagga gtataaaaaa atgctaaaaa
    25501 atatgaacct caaagatggt ttaagtgtga aagatgatta tataatgaga agagttattg
    25561 tatattctgt tttatctgta ctaatagtaa cccttgtatt tatacttttt gaatatagca
    25621 gattacataa attgtatgaa catcaaataa aaaatactca aaatattgct ggaatattga
    25681 ttgaaaaata tcctaatgat gaaatagata tagtaaattc tatttataat tccgattata
    25741 gtaaggtgtc tactggagaa aaggcattta agaaatttgg ctatggattg gaaaataaaa
    25801 tgtcaaatga taaaaatttt agtttatact taaaatcttt ttttaaggaa tcatttttta
    25861 tttttttaat gatgtttata gtagttatag gtataatata ttattttata agatacataa
    25921 ataaaagatt gagtaaaatt tattttatag tagataagat ggcacaagaa aactacttga
    25981 aggacactac tgagtcagaa gaaactggtt atctagtaga atataatgag tattttaaag
    26041 aaggtacatt tagtaagata aataactgtc tatatgagct taatagaagt ttaaagataa
    26101 agtttattaa attagaaaaa gaaaaagaaa gtgtaaaatc attagtaaca gatatttctc
    26161 atcaattaaa aactccttta gcatccttaa aattatacaa tacattactt atagaagaaa
    26221 aattagaaga agatgaagcc aacgagttct tattaaccaa taaggcttct ataaataaat
    26281 tagaaaattt aataaattct ttagtgaaca tatcaaggtt agaaattagc atgataaaca
    26341 taaaaaaaga agataatgat ataaaaaata ctattctaaa tgcagtaaaa agtgttaccc
    26401 ctaaggctaa gagcaaaagt ataacattaa atttaaatga atttaacagt agaattatac
    26461 cacatgataa aaagtggact gaggagagta tttttaatgt attagataat gctgtaaagt
    26521 acacacaggt aaatggagaa ataaatatat ctgtagaaga aactgctaat ttctttaaaa
    26581 ttataataga ggataatggc atagggattg aaaaaaatga gtttaataat atatttaaac
    26641 gtttttatag aggttctgct gatgaaattg aatctgtaga aggtagtggg gtaggattgt
    26701 atttaagtag aaagatttta gaagaacaag gtggaaatat tatagtttct tcaaaagtag
    26761 ggatagggag taagttttct ttgtttctta caactatgta atattaaatg aaaggaaaat
    26821 gtaagcctct acatttatta tataagtata aacagctcca gaaataaaaa attaataaaa
    26881 tatggggtaa tataacttag aatgagggga taattttatg aaaatactat atactgaaaa
    26941 tttatcaaag cattatggaa aaggagaaaa cttagtaaga gctttggaca atgttaactt
    27001 agaaataaat gaaggtgaat ttgttgcaat aataggaaaa tcaggttctg gaaaaagtac
    27061 acttttacat atgattggag gtcttgatat acctacatct gggaaggtgt atatagataa
    27121 taaaaatata tttacattaa aagaagaaga actagctgtg tttagaagaa gaaaaatagg
    27181 gtttatattt caaagctata atttaattcc atctcttaat gtatgggaaa atgtagtgtt
    27241 gccaatcggt ctagatggta gagaagtaga tgaatctttt ataaaagaac ttttgaaatc
    27301 attaggactt gaaaataaac atgacgtttt accaaataca ttatcaggtg gtcaacaaca
    27361 aagggtggca atagcaagag cattagctac aagaccagct ataatacttg ctgatgaacc
    27421 aactggaaac ctagattcta aaacatctga tgaggttatg agtatattaa aatctatgtc
    27481 taagaagtac agtcaaactc tagttatgat aactcatgat gacagtatag cccaaatggc
    27541 tgatagagtt atattcatag aagatggcag ggtgtcaaag gttggtgata aaaatgatta
    27601 atactactaa aatagcaaaa agtaatttaa aacaaaataa atcaaaaagt attctaataa
    27661 taatcactat aatactttct actttaatgc tttcttctat tggaatttat attgttgatg
    27721 caggagcata tcaaaaagaa aatactataa agtattcagg taattatcaa ggtatattag
    27781 caaatgtaga tgaaaaacag gctgatatat tgagtaatca tgcagatgtt gaattaactg
    27841 gtgaaatgaa tggagtaggt gttgaaaaac tggaagatga ttcaaatatt tctctagcat
    27901 acatgaatga agatgctttg aaattaaata gttttgaact tatcaaagga aaattgccaa
    27961 caaaagagaa tgaaattgtg ttagatagtg gagctttaaa ggcacttggt tataaaaata
    28021 aagacttagg tgaaaagata aaaatatctt ataatgacta taaaaatgat aaaaaaattg
    28081 aaaaagagtt cgttataagt ggaatcttaa aaactagcga aatatctgaa gcaggtaaat
    28141 attattatgc tattatatca gaattatata tgagaaatac tagagatatg tcacaagaag
    28201 attttaatat ttatgtaaaa tttaatgata aaagtaattt atcaatagag caagcaaaag
    28261 aaaaattaga taaaattgct aatgatatag gacttgatac tattaatact gctgtaaatg
    28321 aaaattatat aaatgcttta aaaccagata tggaaacgat tatgggtgga ggttttgtag
    28381 gattagtaat tgtattatct agtattttag taatatacaa tatattttat atttcaatag
    28441 ttacaaaagt tcaagaattt ggaaaactta gagcaattgg tgcaactaag aaacaaataa
    28501 aaaatatagt gtttaaggaa gggtttatat tagccggaat agcaatacca ataggaataa
    28561 ttttaggcta tgtacttgca aatattataa taaaatcatt tatggatata gatgtaaaat
    28621 catcacgatt accagttata ttatcggttg tggtaataag ctttatatct gttgttttat
    28681 cacttttaaa accaatgaaa gtagcatcaa aagtatccat tgttgacgca gtaagatata
    28741 caggaaataa aataagtaat aagagtaaga gaaaaggata taaaaatata aatctaaaga
    28801 gattatcaca tgccaattta gaaagaaata aaaaacgtac ttacatgaca ttggcatcac
    28861 ttatacttag tggaactata tttataacag tatcaactgc tttagaaagt tttgatgctg
    28921 agaaaatggc aagagaacac tttccttatg atatagaagt tagactatct ggttatgaaa
    28981 tgaacagtga taaaaatcct aaaaataatt tgaatatttt acaaatggat aatcctctaa
    29041 gtaaagattt ttttaatcaa ataaagaaca tggaaggaat taaaagaata gaatctgcaa
    29101 gaagtgtaaa aattggtatg gaggactatg atgttgaatt taagtatgac cttttacaaa
    29161 gtataaatga aaatgatgta aaatcattaa acaagaatct aatagatgga aaaataaatc
    29221 ttgaaagact gcaaactgga gatgaaatag taattactca tgttgataca gcaaaagaaa
    29281 tgggagtaaa agcaggagat aaaataagat taactttata tgatggagat aaaaagataa
    29341 aaaaagaatt taaagttcaa gcaatagcta tgggggttcc aagttttggt atagggaagg
    29401 attttattga tagaacttta aaatacgatt caactagtag cttgggaata tatacagaag
    29461 aaggaaagta tcaagaaatc aaagatagta tcaagaagat tgctaaaaat aatggatttt
    29521 tagagactga ttttatagat agtagaattg agtcaaataa ggctgttatt tcatttataa
    29581 agataatggg atacacgcta acaggcataa taggtgtgat tggatttatg aatcttgtca
    29641 acacaatgat aactagcata gttactagaa aaaaagaact tggtatgctt caagctattg
    29701 gtcttactaa taaacaatta gtgaaaatgc ttaattcaga ggcaatgtat tacacttctg
    29761 gaatgatgat aggaagtatt ttatttggag gtatattagg gtatattgct gttatatttc
    29821 taaaaaagac tggattatct tatgcaacat attctttgcc aatagtacca atcttgttga
    29881 tgattgtgtg tattcttata gctcaattta taacaactta tttgatagga cgaagtttta
    29941 ataaagaatc tcttattgat agagtaagat acagtgaata aaatgcaaat atttgtggtt
    30001 agtttaacta gcaaaaagca tataaaaaat atagttaaag agattcataa ttaaataggt
    30061 taaagcatag tacagagcaa acaatttaaa cggatgtttg ctctgtattt tattatgtaa
    30121 tcaataatat atctaatata ctgatattat tcatgttaga aaatgtatcg gtacaattct
    30181 aaaaaagtat aaaattgtac catataaaat gaaaattttg tataataatc gtatagaaaa
    30241 taaataggag ctgatatata tgaataagaa gattaattat gcaccaggac caactgaaac
    30301 tagggaaaat gtaagattag caagagcaga aaaaacaaca aatccagata tagatattga
    30361 ttttgttgaa ttttataaaa agacatgcga gaagtttggc aatatagttg gaacaaaaaa
    30421 cgatgtttac atattgagtg gtgaaggaat attagggctt gaagcagcat gtgcatcact
    30481 taccgaaaaa ggagatagag tacttgtaat tgacaatggt atatatggag aaggttttaa
    30541 agattttgta acaatgtatg gtggggagta tgtgcttttt tcaagtgaat atactaaaag
    30601 tatagatatt gatgaattga aagcattttt agatgaggat agtaatttta aatatgcaac
    30661 agtagtgcat tgtgatactc caactggcgt attaaatgat gtaagcaaaa tatgtccatt
    30721 acttaaagaa tatggaatat taactgtagt tgattctgta gcaggtatgg taggagaaag
    30781 attaagtgtg gatgaatcta aaatagatat aatattaggt ggttcacaaa aagctatttc
    30841 tgcaccagca ggacttacta tagttggaat aagtcaagat gcaaaaaatt gtataaaaaa
    30901 tagaaaaact gatgtaatag gtttttattg taatttaaat atatgggaag gctattatga
    30961 aaaaaaatac ttcccttata caatgccaat aagtgacata atggggcttg ataaagcact
    31021 tgataatata ttagaagaag gaatagaaaa tgtattagat agacatgaaa agatagctta
    31081 tagtgttaga aaatctattg aggaatatgg tctagaacta tttttagaag aaggatattc
    31141 taatacagtc acagctataa aaattcctga aagtatagga gctttaaaac ttactgatta
    31201 tatgcttaaa aattataata cacttgttgc aacatcacta aatcaatata tggatactat
    31261 tttaagaata ggtcatatgg gagagaatgc aaatctaaat aaaatagagc atgtactaaa
    31321 tgtcttggat aaaagtttaa gtgcattagg attcaaagga aatgaaaatt tgttgaattt
    31381 atttaataag tattattttt aatagatagg gggatatagt atggaagtta cagctaagca
    31441 aaaatttaca acgaaagaca tggtggaaac ctcactatta atagcattag tatttattgc
    31501 aactaaattt ataaatataa gattaccaat atctataaat ggtgggcttg tgcatttagg
    31561 aacagcaatg ttgttcatat cagcaatagt atttggaagt aaaaaaggtg ctttatcagg
    31621 agctattggt atgtcacttt ttgatttaat atctgggtgg actttatggg caccgtttac
    31681 ctttatagtt agaggagtta tgggttattt acttggaaag attgcatggg caaatggtaa
    31741 taatgggaat aattttttaa tcaatgtaat aggaatatgt gtctcatcaa tttggatgtt
    31801 atttggatat tatataacag aagttatact ttatggtaat tttatagtac ctcttacttc
    31861 aataccaggt aacttaatgc aagtattgat aggtcttata atagcgcttc ctatttctaa
    31921 ggtattgaaa aaatgtataa aataaatata aagtgaacta aaatttatga tagcataaat
    31981 tatactgtat aatattaaga tggagtacat aattaaaaaa ttttagtata gaggttagtt
    32041 cacaatgtta tcagttacaa gatattcaat caaaaatgtt cgtttaaagc cgtgggtaaa
    32101 gtttatttgg tattttgaaa caaaatcaaa tatactgtta aataataagt tacttcctac
    32161 agacagtata gatattattc ttaatctttc tgatgttatg gaatataaaa ttgaaaatca
    32221 agactatact gctagtaata tgcattttaa tggaataagg gataaacatg gatttattat
    32281 tcaacatgga aatatacgtg ttatagggat ttctttttat ccatttggtc tctatccatt
    32341 tttaaagata ccaatttcag agtttaacag acaaattgtt gacttagaag cagtatcaga
    32401 actatttgcc aaaaaattgg aagagtcatt aaatccaaca caatcagtag aaaaaattgt
    32461 tttatattta gaagaaatgt tattatccat attagaagaa gatttaattt caaataaatc
    32521 tgtaaagctt ctaaattcat ttatatatga taatagatat agcaatataa aagcattttg
    32581 tgataataca aatataaata ttaaaacatt ggaaagaatc tgtttaaaat atacagggta
    32641 tacacctaag attttaaaga gaatttacag atttaaaatg gcaagtagtc aacttattta
    32701 taattataaa gataatgaac tttttgattt tatttatgaa aatgagtact atgaccaagc
    32761 acactttata aaagaattta aaaaattttc agggacgtca ccaattaaat ttattggtga
    32821 gaataagacg attaaagaaa atataaagta caactattta taattatact gtcgattttt
    32881 tacaatacct ccaatttatt ttttgatatg atataggaaa ataaattgga ggttttacat
    32941 atgaaaatta ttacagaaat agttgagttt aatgtggaag aaaatttatc aaaagaagag
    33001 tttatagaaa ttgtaaatga cttagaatta aaatttcatt ctattcaatc tggttttatt
    33061 gatactgagc ttttatatga tgaaaagtct caaaagtgga ttatgataca acattgggaa
    33121 tctatagaag acctcaagag tgcttctagt aaaatgttta aagattcttc aactgagaaa
    33181 tttagaaaag ctttaaagaa tcaaactgtt aagatgaata taataccaca aattaaaagt
    33241 tggaaattat agaactgaat gtaaaagata gttaaatagt atcaaactaa attagccaaa
    33301 taacattttg tatttaaatt gttatttggc ttttagtgtt tactttaata tgagtgttat
    33361 atactagaaa aaccaacaaa ctgttagagt gtggattata ataatgtcat atttgggagc
    33421 taaaaggctt aatactgatt tgtcaatatg tctttttagc atgttgaaca ggtatattgc
    33481 catgctttca ttattttatt tttttgcctt atactggact acatttgcag tttcctaata
    33541 tatttggctc atatttaaat gccattatct aaaagtacta ttgctgtacg agaggcaata
    33601 ggcattttag aagaaggagg agttgagcta aagtatacat cataatctct taagttttct
    33661 ttttttgtgt ttcctccaaa ctctaccata tcagtattta atatcgtttt gtagtttgta
    33721 ttttgaaact ttgaaatatc aactgattgt attagattag ggtggaaatt aaatataaat
    33781 aaatatttgc ccttactgta caccaaaatc tttttatcat tatccatatg tataagttca
    33841 tgactatttt ggataaaaat atcactgtat tttgtaagtt cgagcataga tttatcaaaa
    33901 tttaataatt gtttatactt taagttgtta tcttctgaaa ggctccattg tcttctggca
    33961 tacttatagc tccaattgtt gccttctcgt ggaaaatcaa tccactcagg atgtccaaat
    34021 tcatttccca taaaatttag atatccttca cctgctagag aaaaagttat taatttaatt
    34081 aatttaataa gagatattgc tctattgata acatggttat ttgagtttat ttccatattc
    34141 cagtacattt ccttgtcagc tagccaaaaa ataattgttt tatcgccaac taatgcttgg
    34201 tcatgggatt cacaatatcc tatatttttt tctccaggtc gtctagtagt aagttcatac
    34261 cacatttttc ccaaatccca gtcttcatca gataagttag agatagtttt tatccaaaaa
    34321 tcaggtactc ccattgcgag tctatagtca aatccaattc cgccgtcttt tatagggata
    34381 cacataccag gcattccact catatcttct gcaatactta tcgaattagg tttaatttct
    34441 tttattaatt catttgcaaa ttggagatat gttatagctt caatatctgt attcatacta
    34501 aaatattttt cataactatc aaatgaaact ccaagaccat ggttatgata taacattgaa
    34561 gtcacaccat caaacctaaa gccatcaaaa tggtactcat ttaaccaaaa ttttatattt
    34621 gaaagcaaaa aatgtattac ttcaggttta ccatagttaa aaagttttgt accccaagca
    34681 ggatgatttc cctttgaacc tgagtggaaa aattgatgtt cacttccatc aaactcattt
    34741 attccttcta aagtattctt tacagcatga gaatgaacta aatccaataa aacagcaatc
    34801 cccattgaat gagcagtgtt tatgaggttt tttaagtctt caggagtacc aaacctagat
    34861 gagattgcat aaaagtttga aacttggtat ccaaaagatg cataatatgg atgttccata
    34921 atagccataa gctgaatggt attgtatcca gcttttttta tcttaggaag tattttttca
    34981 gtaaattcat tataagttcc aatagattcg ctttcagtag acatacctat atgacattca
    35041 tagattagtg gagaagttat gttttttaag tcaaaattat tgtcagtcca gataaatgga
    35101 gtttttggtt gccatatttg tccattaaat ccaccaaaat ctttttgaat tactcttttt
    35161 atatataatg gtatacggtc aaatgttttt ccatttgcag taacttgtac ctttacctct
    35221 gatttgtgag gtaagctatt ttgaccagga ataaatattt cccaattacc agacccaatt
    35281 tttcttagag gatgagattt tctattccaa ttattaaagt caccaattag tgacaaacta
    35341 tctgcatttg gagcccattc cctatagaag aatccatcat ttgtctgatg aaatccatag
    35401 taaagatgag catttgcaaa agaactaaaa tctttatagt ttttcaatgt ttttgatttc
    35461 atatcctcat aatgtttcat tcgctttttt atatcagatt caaatggttg taggtataca
    35521 tcaatatcta aaattttaaa tcttttttga cttttcattg tatttctcct ccataataaa
    35581 tattacattt aaaaaataat atttatctca tactatcttc tctgtataat tgatatatat
    35641 tatataatcc taataaaaag cttaagccac caatgattaa tttcataatt tacctcccta
    35701 gtatttatac ttatatatcg tattcatcaa aatttaaaag tttgtttaaa actcttttaa
    35761 taatatcata atcaaagcct ttataggaaa gatgttgaga aattttttga tatattttct
    35821 ttttatcttc tttctttact ctttcatacc ttttttttgc tagatacata gcattttcaa
    35881 attcagtgtc cttgtctaat tcagatactg cttcattgat tgtacttcta ttgatacctt
    35941 tattatataa attctgctta attctgtttt tgccacattt atttaggtta acatttgtgt
    36001 taacaatttt ttgagctaat acactgtcat ttacgagatt ataactttta agaaaatcta
    36061 tgaccctctc aattatattt tcatcaaatt ctgatgatag tttttctttt aatttttttt
    36121 cagattggtc agcatgagat aaaatattta atgctttatt tttagctttt atatacattt
    36181 catcatctaa aataggtttt aattcttctt catttatttc attccctttt tttaaattaa
    36241 atgtataaac aagttcttta aatatagcaa taaaaaattt atcatctaca taaatattaa
    36301 ctctatcatc atttcttttt tgttgttcta tttttgtaat tataccc
//
LOCUS       DACTGW010000009        36080 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.009, whole genome shotgun sequence.
ACCESSION   DACTGW010000009 DACTGW010000000
VERSION     DACTGW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.009"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(188..1096)
                     /locus_tag="I9573_000383"
     CDS             complement(188..1096)
                     /locus_tag="I9573_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HAT4795021.1"
                     /translation="MNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKCMNV
                     PIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSINAF
                     IKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINRLPV
                     LIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDIETL
                     NTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKYYLE
                     KTNKSIIEATLLPYSEIIETVKEFWE"
     gene            1367..1744
                     /locus_tag="I9573_000384"
     CDS             1367..1744
                     /locus_tag="I9573_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAT4795022.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            complement(1900..2349)
                     /locus_tag="I9573_000385"
     CDS             complement(1900..2349)
                     /locus_tag="I9573_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4795023.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2527..2931)
                     /locus_tag="I9573_000386"
     CDS             complement(2527..2931)
                     /locus_tag="I9573_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795024.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            complement(3044..3910)
                     /locus_tag="I9573_000387"
     CDS             complement(3044..3910)
                     /locus_tag="I9573_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAT4795025.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRSTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            complement(4480..4824)
                     /locus_tag="I9573_000388"
     CDS             complement(4480..4824)
                     /locus_tag="I9573_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAT4795026.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            complement(5013..5630)
                     /locus_tag="I9573_000389"
     CDS             complement(5013..5630)
                     /locus_tag="I9573_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4795027.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKNLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            complement(5635..7806)
                     /locus_tag="I9573_000390"
     CDS             complement(5635..7806)
                     /locus_tag="I9573_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAT4795028.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNED
                     TINKILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVS
                     REASILRLNGYSKLDIIARVIKNIFRIMILSSVTALLIYIIYILKLNIGVRACLYFAM
                     FSIICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSI
                     NNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLV
                     EEKDAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGK
                     EIFNLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKT
                     KKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIIDIDNIDDSFYLSYISRCV
                     YFNSKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIA
                     ISNLMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRV
                     DLSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            complement(8221..8853)
                     /locus_tag="I9573_000391"
     CDS             complement(8221..8853)
                     /locus_tag="I9573_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HAT4795029.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINLENLHIWLENGVDVCGFGGLLTKGTIEDITENAKKIREIIKNIREI"
     gene            complement(8959..9864)
                     /gene="dapA"
                     /locus_tag="I9573_000392"
     CDS             complement(8959..9864)
                     /gene="dapA"
                     /locus_tag="I9573_000392"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAT4795030.1"
                     /translation="MKYKGIICAMITPFDEKQNINSQATCKLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVIDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEVTQNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            complement(9869..10468)
                     /locus_tag="I9573_000393"
     CDS             complement(9869..10468)
                     /locus_tag="I9573_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAT4795031.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIVLQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            complement(10526..11797)
                     /locus_tag="I9573_000394"
     CDS             complement(10526..11797)
                     /locus_tag="I9573_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HAT4795032.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            complement(11824..12111)
                     /locus_tag="I9573_000395"
     CDS             complement(11824..12111)
                     /locus_tag="I9573_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4795033.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            complement(12126..14207)
                     /locus_tag="I9573_000396"
     CDS             complement(12126..14207)
                     /locus_tag="I9573_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT4795034.1"
                     /translation="MDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKINDM
                     LKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLFIN
                     MEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIRRV
                     LIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEFIY
                     IFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPYNIMYI
                     SAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFRPA
                     YYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHFAT
                     LFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENISEP
                     VDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIYLRQPKIDEVMD
                     IIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNWEE
                     AIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAIGI
                     ATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKEAK
                     EIIDFIKTHEL"
     gene            complement(14164..14313)
                     /locus_tag="I9573_000397"
     CDS             complement(14164..14313)
                     /locus_tag="I9573_000397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795035.1"
                     /translation="MKTIKFSNKISIVEVMKESNDNMKTNLNKKVGVNGSGQRNCINS
                     PDFIK"
     gene            complement(14780..15628)
                     /locus_tag="I9573_000398"
     CDS             complement(14780..15628)
                     /locus_tag="I9573_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HAT4795036.1"
                     /translation="MGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFGPY
                     CTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRWQG
                     CIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGPNY
                     RWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVSEL
                     WFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQYR
                     GYIPMR"
     gene            complement(15666..16178)
                     /locus_tag="I9573_000399"
     CDS             complement(15666..16178)
                     /locus_tag="I9573_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAT4795037.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDKKKGN"
     gene            complement(16171..16395)
                     /locus_tag="I9573_000400"
     CDS             complement(16171..16395)
                     /locus_tag="I9573_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795038.1"
                     /translation="MNEKPSFKQIEIILAVIFLVLTYYKIGLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            complement(16455..17408)
                     /locus_tag="I9573_000401"
     CDS             complement(16455..17408)
                     /locus_tag="I9573_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT4795039.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLESGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            complement(17436..18824)
                     /locus_tag="I9573_000402"
     CDS             complement(17436..18824)
                     /locus_tag="I9573_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795040.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPNNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKAGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            19097..19978
                     /locus_tag="I9573_000403"
     CDS             19097..19978
                     /locus_tag="I9573_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT4795041.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            complement(20165..21157)
                     /locus_tag="I9573_000404"
     CDS             complement(20165..21157)
                     /locus_tag="I9573_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAT4795042.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKKIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(21162..21902)
                     /locus_tag="I9573_000405"
     CDS             complement(21162..21902)
                     /locus_tag="I9573_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAT4795043.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLVGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGDTKVEDIGVLYKIMKELLG"
     gene            complement(21967..23076)
                     /locus_tag="I9573_000406"
     CDS             complement(21967..23076)
                     /locus_tag="I9573_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAT4795044.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFL
                     IIEELVNKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEINFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDNIYNRIKNIVES"
     gene            complement(23094..24227)
                     /locus_tag="I9573_000407"
     CDS             complement(23094..24227)
                     /locus_tag="I9573_000407"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAT4795045.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNHIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGEFY
                     EDTKKYKTNVYALINIAKQGITSQDELSNMMNIDEYELKNAVRKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEAKERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGNSIEENE"
     gene            complement(24229..25323)
                     /locus_tag="I9573_000408"
     CDS             complement(24229..25323)
                     /locus_tag="I9573_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAT4795046.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMVSKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAKEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNIFKRGDVVIGNDDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            complement(25460..26761)
                     /locus_tag="I9573_000409"
     CDS             complement(25460..26761)
                     /locus_tag="I9573_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT4795047.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKATLDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            complement(26985..27686)
                     /locus_tag="I9573_000410"
     CDS             complement(26985..27686)
                     /locus_tag="I9573_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4795048.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            complement(27899..28216)
                     /locus_tag="I9573_000411"
     CDS             complement(27899..28216)
                     /locus_tag="I9573_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAT4795049.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SKEVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            complement(28264..28575)
                     /locus_tag="I9573_000412"
     CDS             complement(28264..28575)
                     /locus_tag="I9573_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4795050.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            complement(28724..29203)
                     /gene="rlmH"
                     /locus_tag="I9573_000413"
     CDS             complement(28724..29203)
                     /gene="rlmH"
                     /locus_tag="I9573_000413"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAT4795051.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIVLAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            complement(29271..31373)
                     /locus_tag="I9573_000414"
     CDS             complement(29271..31373)
                     /locus_tag="I9573_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HAT4795052.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEKEI"
     gene            complement(31386..32183)
                     /locus_tag="I9573_000415"
     CDS             complement(31386..32183)
                     /locus_tag="I9573_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT4795053.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            complement(32285..33748)
                     /locus_tag="I9573_000416"
     CDS             complement(32285..33748)
                     /locus_tag="I9573_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HAT4795054.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            33873..34931
                     /locus_tag="I9573_000417"
     CDS             33873..34931
                     /locus_tag="I9573_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAT4795055.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            34940..35932
                     /locus_tag="I9573_000418"
     CDS             34940..35932
                     /locus_tag="I9573_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT4795056.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKEKLLSESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
BASE COUNT        11953 a         6278 c         3993 g        13856 t
ORIGIN      
        1 ttagataagt aacaagaaac taacatgtaa tgttaggtac tctagatatg aaatcttaaa
       61 acttttaaat attgaaatat ttctttatat atattattgt atatttctta taaattattg
      121 tcaatttatt ttttcttaaa aataaaataa ttgcataatt tatttcaaat tatgcaattt
      181 ataaaattta ttcccaaaac tcttttactg tttcgattat ctcactataa ggcaataaag
      241 tcgcttcgat tattgattta tttgtctttt ctaaataata cttaatcata taatatccac
      301 aagcatatcc tgcacaataa ggcaacccta ctggaaaata tccttgcatt ttagctatat
      361 catcaccata aagataagat gttatatttt gaaatccagt ttcttttaaa gcacttttta
      421 ttattggctt tatataagta tttaatgttt cgatatctgt tctactgacc caaggtccta
      481 acatttcctc tccaaacatc catgttgcaa aattttctgc aagaccttca tttatcatca
      541 tttcttctaa tgttatatta ttattccact ctataaactg aaatctaata ttgtgattac
      601 attcatgtgc tattaatact ggtaatctat tgatagtata ttcattagga accaatgata
      661 gaaatatata tccaggaatc ccaccatcac cccagtatcc atcacttaat attgtataag
      721 gactatttgg attcgccaat aatattgaat atttatagtc cttaatgttt aaatcatacc
      781 cttcttttat aaaagcattt atagagtttt ctatggtttc tttgcaagtg ccccaaattt
      841 tatcagcaga taacacattt atattctttt tttgcttttc atcaatatcc ttaggcgata
      901 aaacccctaa catttcactt gctataatta catcatatcc acctttctgt ctagcaacta
      961 ttggaacatt catacacttc cacttaaatt caaaaggctt catcaattca tatctgtata
     1021 tatcttcttt tttgtttatt ggtgcatcca ttatcttctt gtatatttta tctgagcgaa
     1081 tttctataat attcatattt accatctcct ttaagacaaa gtatatacaa ttacgtagcg
     1141 tcaatgtcaa tatacatttt ttataatgtc accttaatct ttttattata ttttatcctg
     1201 taaaccttcc gtacatataa cattttttta aattaataaa tttgtataat ataataataa
     1261 aaaattatat acttcaaggt ttccctatga tacaatttac taaatcataa tttagattat
     1321 caggtgcaac tataaattat tctatatcaa ggaggatata ttggtcatgc taactccata
     1381 tttaatattt aatggtactt gtgaaaaagc atttaatttt tatgctgagg ctttcggagg
     1441 aggaaaaact atatttgcgc gattagacag caatccaaac aatcctatta tgcacgcaag
     1501 tgttactttc acaaaatacg aaggttgtat aatgggtgcg gatacagaca agcctgttgt
     1561 aatttctggc atggcgattt gtgttgttct accatctcga gaagcgatag aagaaatatc
     1621 tgtaaaactt gccgagggtg gtacacttgt acaagaattt ttaccacacc caccaccaca
     1681 tcaaaatgat ggcgctgctg aagtacttga taggtatggg tatacttggt atttaagtac
     1741 atagtaaaaa taaaaaaact acatctaata atcataatat agaacagata aatttcaatt
     1801 taatagtgta gtaaaaaagt cgtgtcctaa gactgtcacg acttttattt gtataaaaat
     1861 ttaagtaaat atttttgcga ataagtgaga agtataactt taaattaatt tcatatgtag
     1921 tattggaaat gggttacctt gtccatctat ttcagaccta tcaaacactt taaatcctaa
     1981 gtgaatataa aatcctacag cttttttatt ctgctcattt acatcaacat attttacatt
     2041 gtatttatct atagcatatt ttattaattt tttaccaata ccatttcctc tatatttatc
     2101 ttctataaag agcatctcaa ttttatcttt atctattcct ataaagccct ttattatatc
     2161 attatctttt atacaaacta tattttctac ttctttaagt ccaatttcaa ctaatggtat
     2221 caaagattca atatcttttt ctgttaaaaa atcatgcgtt gctcttactg atgattccca
     2281 gacttgcaac atttcgtcat attctgttga atttatttgt ttcatttcaa gaattattcc
     2341 agatttcata aaatttcctc cctatatcac ttttaattgt ataacacttt tttatacaaa
     2401 acttagaaag gactacgaag gttaagtatc atagtcctaa ttaagtttaa atattatcta
     2461 ttatatattt ttaacattta ttatatacta taatctagca aatttactac aataatccat
     2521 atcagtttat actaaaggtg cttctatttg agtagtaaaa aggaatttat gacaatgacc
     2581 atcattaaca gtagtttctc cacatattaa gtgaatgtgt ttatcagttc caggtattct
     2641 aattgctggc cctgttgtaa cacatatctt atggaaatgg tcaaggaaat ctacattatc
     2701 atttatttta tgaacatgat ttgtacctcc atttattggt atagcttcac cagtaacccc
     2761 tgctgcacgg tggttatgtc tatcctcaca ttcttctgct aacttaacac tttctgaata
     2821 ttcatgaaca tgtgtcattt cctcataatc attatagcac ttgttgcact tattgtattt
     2881 ataatcatca tacacataat ctctttcaca tttataattt tgataactca tttttatatc
     2941 tctccctata ttatattaag aaattcttgt aaagaatctc taatgtataa tatgaattaa
     3001 agtcaaaaaa gttcatattt tgtaaaatta tgtattaatt taattatttc tcccagtatt
     3061 tagggcgtat tagtgaagct ggtaacttcg tactagaatc tccttttgca gtatttactt
     3121 gcaattgtgt taaaaatata ctattttcaa gattagcacc tctaatatca caatctctta
     3181 agtctgctcc tattaaattt gccccactca aatctgtact tcttaaattt gctgctatta
     3241 gaaatctacc tcttaaatct gctcctatta gatttatttt tctaaggtct gctcctatta
     3301 aatctatatt tttacttaat tttttcatat ttttaagagt agaagtcttg ataaatgatt
     3361 taacctttcc catcacagac tcactcgctt gtgataataa taaattaacc tttgttctat
     3421 gtgatatgat atccaaatta attatctgtt ctgggctcat attagttatc ttttcagttt
     3481 cttcaattat caaatcaatt tttgatttga ttgtatctga tatatttaat acagatgctt
     3541 ctttaagata ccataacatt tcatgtaatt gtctcataat taaaaacact tcagacattt
     3601 gatttgttaa ttctggtgat tgcatccagt ctatcccctt atatgttact tgtgctactt
     3661 tttgacctga accaaaacaa tcatatgcag tacacccttt aaaacctcgc ttcataagac
     3721 tattatgtac agaacattta aaatctggtt gtaaattaat acagggtttt cctgattcct
     3781 tatcgatagg aaatccatct gaagcagaaa aataaagagc tacacaacaa agtccaaaac
     3841 acttcttaca atcaatcttt agctcctcaa ataacttatt atcatatata tttttatgta
     3901 tatctatcat attttttctc ctcttaagct ttatttttaa tattatatta tctataacta
     3961 aatgataatc aagtccctta tttaacagtt cttattctta attataattt aaggtattat
     4021 ttattccaat agcatacata agtttcttta tacttgtaac aataccttta tactaagact
     4081 tattttaaat gtagcactgt aataataaat tgtttatata ctcataatgt atttgataaa
     4141 ttgtaaatct ttatagaagt tgaaattttt aatgatttta gaatcaagat ataataataa
     4201 atacaattta taaaatcaga aatatataaa ttaactatac aaacctaaat aacatagcat
     4261 ttaaattcac aatttcagta taaatagtac aaagtatatt aatttaaata agattggctt
     4321 atttgtagag gagagaatat tttggtgttc aataaaattt atatttaata aaaccataag
     4381 aaaatataaa gataaataaa caaactttta ttacttaata gattcttatg gttttatact
     4441 gtactgaaaa acaataaaca ctacagctta tttataatat tacgcttttt taacaacaga
     4501 agactttagc ttcatagctc caaaaccatc tatcttacat tctatatcat gtccatctga
     4561 tgagtcatgg attaaacgga tatttttcac ttttgttcca atctttatag atgatgaact
     4621 tccttttact ttaaggtctc taattactgt aacagaatct ccatcattta atacatttcc
     4681 atttacatct ttgataatat ttgaatcttc ttcagctccc aatcctaatg cccactcatg
     4741 agagcattct ggacaaacta aaagagttcc atcttcataa gtatattctg aattacattt
     4801 cgggcaattt ggtaaatttt ccatctttat atgtcctcca tttgttattt ttaaatttct
     4861 tattatataa aatttctttt gagcctttat gatatcatat tatacttata tttacaaact
     4921 actaatttac ttaatgttta aattctattc gtattattat taaacttgat ttagctgtaa
     4981 tctctttctt atatttatta tagttgatat tattacaatt ttataactct atctgcctgc
     5041 tccgataact ctaaatcatg agttacaaat acgatagttt taccattctt atttagattt
     5101 ttaagtatat caaatactat gtctctattt tttttatcta aattacctgt aggttcatct
     5161 gcaaatatta cttcacattc ttttaatatt actcttgcta atgctaccct ttgttgttct
     5221 cctccactca attcgtatat ctttttttta ttgtattttc taagacctac agactcaagt
     5281 gcgtaattta ttttttcatt atggttgata tttttttgat actttaaagc aattttcaag
     5341 ttattttcaa cattttcatt ctcaattaaa gcataatttt gaaatagata gccataaaaa
     5401 tccctttgta aaattcttat ctgtttacta tttggatttt ttatattatt tattataata
     5461 tcaccagaat taggtttttc taataatccc attatattaa gtaaagttgt cttacctgaa
     5521 ccactatttc ctgttatcgc tacaaactcg cccttagaaa tttctaggtt aaatttgtct
     5581 attatagatt tatctttaaa ttttttagat atatttatta atgaaatcat ctttttaatg
     5641 ttctcctttt ataattttat taaatgaact atttgagata atcttgtcta atacaataga
     5701 tacaattact tctataacta atatcagaag tccaaataag ataatattat tacttaaatc
     5761 tactcttgtt gatataatgc ctattggaat aatgtttgtt aatattaatg agactattaa
     5821 caacatactt ctagctgtag ttgaataacc aaatattttt ttaatatata acttatattt
     5881 atttctttca tagtagctag ctactatatt gtaggtaatc attaagtttg atattgctat
     5941 tattattata gtaaaaattt cactattcaa atatttttct agtttattta tttctaaacc
     6001 atactcatta taaacagcat gtaaactttg gatatgcgct tctacacctt gagcttcaat
     6061 aatgttactt atatctgcaa gggcatctaa tttcttagaa ttaaaatata cacatctaga
     6121 tatataagaa agataaaaag aatcatcaat attatctatg tcaattattg ctattgggtc
     6181 atcaatgaga ttcctattgt catccataac aagggagtta taagtaaaat agctttgatt
     6241 attatctaca tatattatat taacatttaa atctgacttc tttgttttat ttaactctct
     6301 atttaactct ttattgtaca tattttctac ttctattttt tcaaaataaa ataattctct
     6361 aaactctttt ttaatctcat tttcatgtac ttgtaacttt ttaggaacta ataaatttat
     6421 agtcttatcg tcataatcga ttagattaaa tatttctttg ccatttgagt aaatagggtt
     6481 aactttaaga tagtttttat ttatttttat atttttacca tattgactta cttctgggtt
     6541 tttacccttt gaatttattt catacatgta acttccatct tcaagttttt caaaattagt
     6601 tgaatctatt aagaaagcat ctttttcttc taccaaattt ttatagaaac ctttaatctt
     6661 ggcattttta attacttctt cctttcctaa agattgttcg cctgtatctt taagagtaag
     6721 attataaaca ttttttgctt tatcccactc agatagattt cctaactgag tttgtagcat
     6781 attataattt aaaatacagt tatttatgga taatataaga aatgatacaa atacaatttt
     6841 caatgtgaaa tgcattatat ttacaaaacc ataagatttt tttccattta gagatagcac
     6901 atatttagaa cttctactat taaaaccaac tattaatata gatatcaaaa tatttatcaa
     6961 aatacatata atcgaaaaca tagcaaagta taagcatgct ctaacaccta tatttaattt
     7021 taaaatatag attatatata ttaataatgc tgtgacagat gataaaatca taattctaaa
     7081 tatatttttt ataaccctag caattatatc tagcttagag tatccattta atcttaaaat
     7141 agaagcctct ctactcacac taattgaata atgtattatt gtagccaaga aacaaaggat
     7201 aattagtatt aaatctctaa ttattgaagg atttaagtat aaatttgagt taacatatct
     7261 atcatgtaat cttgctacaa aaatacttct gtctacattt agttctctta atattttatt
     7321 tattgtatct tcattttgag ttgaaatata taagattcct gattctccaa cttcacttat
     7381 tttaccaaaa tccctaatta aaattgatat gtctttactc atagttgaga atttacctac
     7441 ttgattacta ttattactat tattagtgtt tatattacta ataaattcat ttgaattagt
     7501 attaggaaat tttcctgatt ttaactttat attgttattc aaacttgtat cagtcgtata
     7561 tatagataat ttttcattat tattaaaaac atactttgat atagccacat tattttcatc
     7621 tgcaatatct ttaatccact tactataatt tttatttaac tttttataat ctttaaacat
     7681 tacagatata cttgttgtat cgttatataa aatattatta atttcattat ctttaactat
     7741 atttagacct aataaagata ctattaatag ttcaattgta aataatacat atattatctt
     7801 tttcaattat agctccctcc taattgtttt tattctaaaa gcaaaatata ttttacttta
     7861 gttaaagtat aatgctttgt accttttcta aacaactact atattgtcac attctaaatg
     7921 gcaagtgtct atttcgtcac aaagataaaa ataaccttaa aacagttaat tttttataat
     7981 caactgtttt aaggttattt tttattttta gcaaacaata ttttaatagt ggtacaaaat
     8041 tagtataatt cattaatata gattctagta tttttagcaa tcaactaaaa taatatctta
     8101 tttaaaacaa aatttaattt aagttcaata taaactttac ctcaactaga aaatatttct
     8161 tctaacttag gaattaatgt gtggatatct cgtcaataga aaactaattc atcctccatg
     8221 ttaaatttct cttatatttt taatgatttc acgaatcttt ttggcatttt cagtaatatc
     8281 ctcaatagtt cctttagtta ataaaccacc aaatccacat acatcaactc cattttctaa
     8341 ccatatatgt aggttttcaa gattaattcc tccacttgct aaaactggca tatcaggtat
     8401 tggtgtctta aataccttta ctagtttagg tccatagaaa tctgatattg ggaaagcttt
     8461 tataaaagtc gctcctaaac ttagtgcatc tactgcctca gtcaatgatg tacatcctgg
     8521 agcataagga atctgatatc tattgcaaat cttcgctaca ccttcattgt aatttggtgc
     8581 aataataaat tgagctccat gtaatatagc atgacgagct gtttcactat ccaaaactgt
     8641 tcctgctccc acacaaagta tttcaccata ctttttatta agcccttgaa ttatttctcc
     8701 agcattattt agggtataac tcatttctat aactgttata ccacctttta tacatccttc
     8761 agcaatttca catgcacggt taagattttc ttctcttaca attgcaagag ccccacattc
     8821 tgacattctt ttggttatat tcatcttttt catattatca cctctatact catcaaacca
     8881 ctttacttat tcattaattt tagtcatgtt ttataatata tatattaatt atataccagt
     8941 gtaatttccc tatttatatt atctattctc actactttta ttaagtattg tcctataatt
     9001 ttcaactact cgtttaattt cttcaagtac ttccccagtt ggttcagtta caggaagtcg
     9061 tgcagttcca acattaattc cattcaagac aatagttttc ttcataacag atggaattgt
     9121 tccaagtttt agtatacgtc gaagttcttc tatactactt tgagcttttt gagcttcttc
     9181 caaatcccct ttcataaagt tttcatagat agaaatacca atctctgtga ggaagtttgt
     9241 tgttgctgca acagctcctt gactaccagc ttttagacta tccagaataa gtgaatctga
     9301 gcctgaaaac acactaaaat tctcattctg cgttacttca atataagctt tcatgttgtc
     9361 aagtttacca ctactatcct taattccaac aatattcttt atctttgata attcacgtac
     9421 tgactctggt tcaatattaa ttccagtctt acctggcata ttatacatta taataggtaa
     9481 attaactgaa ttagcaatag ttttgtaatg taagattaat tcctgttgtg ttggtgtaac
     9541 aaagtatggt gtaataatag ataatgcact tgctccaatc gcttccattc tcttggataa
     9601 atctataact tcacgagttg aattaccacc tgttccaaca aaaacaggta cacgattatt
     9661 tgtatgatta ataacaatct ttgcaaattc aactttttca tcatctgtta acacatgaca
     9721 ttcaccatta gtacccaaaa taaataatcc ataaatcccc ttttcaatca aataatcaat
     9781 taatttacat gttgcttgtg aattaatatt ttgcttttca tcaaatggtg ttatcattgc
     9841 acatataatc cctttatatt tcataaaatt atattccctc tctaatagac tttccaagac
     9901 ttcctgctgg atgccatttc aaatactgct gtgtattaag acctttagca tattgtaata
     9961 caacacttaa tgattgtaat actattgttt cagctaaaat tgaagcacga ggagctttat
    10021 taagacaatc tccttcctgt ttaacgcctg cgaacaggaa tatatcagaa atatttttta
    10081 aaaatgaact ctcattacca cttacaccaa ttatttttgc tccatttact tttagagtct
    10141 taagagttgc ttttaactct tgagtttcac cactatttga tattgcaata acaacatctc
    10201 cttcaactac ttgaccactt gacccatgta ctgcttctgt accatgcaat atatatgctg
    10261 atgttccagt agatgaaagc aatgatgaaa tatatcctga tacatgtcct ggtttaccaa
    10321 taccagtcac atgtacacgt ccgtgatttt tttcagcttc aagaattaat tcgcatgctt
    10381 tatttataga tgtctcatct attgaggcaa taaattctga caattcatta gatatattat
    10441 ctaaaaattc agataatgta ttttccataa gatgtatcct ccttgaaatc atagtaacta
    10501 tgactatact tttatataat ttagtttaaa taggttttat aaattgtaaa atattatgta
    10561 atatcgaacc aactacgttg taatcaatat tagggaaagt tgctccttct ataccaagag
    10621 tcccataaac tggatatagt ataactggta aaaatgcaag taataaccca acaacaaatg
    10681 acccagcaat agcccctctc catcctcctg aagtattgcc aaatattgca gctgtacctc
    10741 ctgagaagaa acatatgtga gcagctggaa taattactac aggaaatata ctaggaaatg
    10801 atgccataat agcaattgct aatagaccag cagcatatgc tgatataaac ccaataataa
    10861 ctgcatttgg tgcatatgga aatactgttg gaacatctag cgcaggacgt gaacctggaa
    10921 tatacttttc tgaaatgatt acaaatgctg cagttatttc agctaagaac atacgaacac
    10981 ctgacatcaa tattgacata cctgccacaa atgtaaatgc ctctataagc ggaaaaacca
    11041 accaatgtgt tgaaccagca atttcttgag taaatgcttg accagctttt aatgttgcaa
    11101 tatagaataa gattaacata actatggata gacccattaa gaagtctctg aatattgata
    11161 accacttagg aaattctata gtctctgttg tatcatcttt atgttttgaa aataaacgcc
    11221 caatataacc tgataaacca taaccaatca tgttaaagtg accaatagct tgttcgtcgc
    11281 cacctgttaa ttcacgcata aatggttgac agaattgagg tagtgcagca gacataaacc
    11341 ctaagatagt tccacctatt aaaatagtga ttgtattatc aaatccatgt gctttcatga
    11401 ttaaaactaa tacacaagaa aagaataaac tatgacctgt tgtaaagaaa atattcttaa
    11461 actttgtaat acgtgcaaat actaaattta atatgaatcc aagaattaat gtacatgaaa
    11521 ctgcaaaacc atatgtacct tgtgcaagtg ctgtagctgc ttctggtgaa gctataacac
    11581 cagtaattcc aaagccatgt tggaacagtt tattgaaatt ttgaagtgct cctgtcatag
    11641 cagaggcacc aataccaaat atcaaaaaac cgataagtgt ttttgaagac cctgtaaata
    11701 cttcaacggc tgatttcttt tgaagaatca atcctataca tgcaactacg ccaagcaaaa
    11761 cagcaggtgt actcagcaaa ctaataataa actccataaa tattcctcct tatcatatta
    11821 aatttatccc tcttttgaag caaggaattt ttctagtgct gtctttattt cattacggtc
    11881 aacaatattg tgaatcccaa ttatatcaaa cttcaaattt ttatttttta attcttcagc
    11941 aacatcaatt aatgtttcta cagcatctcc attgaatcca tttagtgaat ccaatgaacc
    12001 atgttctact tcaattggaa agttattttc cttaataatt tgttgagtct ttatttttaa
    12061 cattaaactt gagccaactc ctgctctaca tgcgattaaa attttataca taattaaatc
    12121 ctcctttata attcgtgtgt ttttataaaa tcaataattt ctttagcctc tttagcacta
    12181 tctaaaacct taaaaaactc atcttcttcc aatagttcaa ctagctcaga catagcacgt
    12241 aaatggctat tattgtctat tgcacttaga caaaatacat attttacagg gtcgttttcg
    12301 ttattcccaa actcaatagg actatctaaa gtagcaatgc ctattgcaat ctcttttgac
    12361 ccagcttctg gtctagcatg tggaagtgca acatgttttg taattactat gtatggtcca
    12421 gatgcttttg ctgagttaat catagcatct acataccctt cagttacttt gttattttca
    12481 actaatacag aagctgactg tctaatagct tcctcccaat tcttagcttt tacctttatt
    12541 tttactagtg tttcattagt aatctcactt agcataggcc cctctccttc cttagatgta
    12601 aaattctcta tctgtgtaaa ataagctaat agctcacttg aaagaatact ttcagaggtt
    12661 acatttgcat atttttttat tatatccata acctcatcta ttttaggttg tcttaaataa
    12721 atatcaccta attgaatata cacttcacgt gtaatcttat atttttcttt tgtggtcata
    12781 acagggctta ctttaattac tggtacatct gtttttaaac cctctgcgtt ataattagta
    12841 gtaaatacga tatctacagg ttcacttata ttttctaatc caactgattc cgacattggt
    12901 ataaagtgta agtcaggaaa taagtttgta agttctgcat gtaaaatagc tgaactacca
    12961 acgccatttg aacaaactat taatgctatt ctttttctaa aaccatcaaa ttctttttta
    13021 tttgaaaaaa gtgttgcaaa gtgcattgta agatatgcta actcttcttc ctgaatttct
    13081 tctccaaata atccgcaaaa ctctttcatt gcattactta caattctata aactaattta
    13141 tattcttctt ttactttttc acaaatcgga ttatagatag gtaatttaaa taaaagtcta
    13201 tagtaggcag gtctaaaatg agaatataat tgtttaaata ttttttcttg gcttatatag
    13261 taaactccgc ttaaatatcc aaactttgtc atcattttat ttacaatttt tgaaattaca
    13321 tccttatctt ctgtatcttc ttcataattt cctaccgata tacctaaaat ccaagcacta
    13381 atatacatta tattataagg ctttatctta tcctcactcc catatacttc taataattcc
    13441 ttagcaaatt tatactcctt cattgaactc ataactgaaa tattaggaat atctacattt
    13501 tcctgagtaa ctattctagt tgaatacatc cttgctttta gaaagataaa tatatatata
    13561 aattctatta atctatcttc aacaaaagta atttcatatt tttttattag ctttaatata
    13621 atttttttag cctcttcaaa actatctaaa ttatattctt taataaaaat atcaaatact
    13681 cttgaacttt tctcattaga caaagtttct ataatgtttt taataagcac tctcctaatt
    13741 tccatctcat tgccaactat gtaatatcca tttatacgat tgttcttaac tttaatgttc
    13801 ttcttttcaa gctctggtat caaatccttg aaatcaagat ttacagtaga cctacttact
    13861 tttatagaat ttataaaatg attaattgaa atatcctcca tgttaataaa aagcattaaa
    13921 tacatatata aaagtctttc tgtcttacta aaataataag tattcttaga atagtttttt
    13981 tctaaaattt ctttaattgc tttttttgtt tcttttctaa cgataatatc tttatcagct
    14041 cttaaacata taagtggtac attttttact ttaagcatgt cattgatttt atttattcta
    14101 taagtaattt gtctctttga tgaattagac tcctcttgta atccattagt tgtaatcaat
    14161 gtgctattta ataaaatctg gagaattgat acaatttctt tgtccactac cattaacccc
    14221 tactttctta ttaagattag tcttcatatt atcattggat tctttcatca cctccacaat
    14281 acttatttta tttgagaatt ttatcgtttt caatttcatt taagtataac ttttgtttta
    14341 gattgagaca aaaattaaac ttatctaaaa tagctactat attacactta tagattttat
    14401 aaataaaaaa gggttcctta tgtaaagaaa ccaaaaaaaa tttatttaaa gaatcaaagc
    14461 gatactaatt atgcataaat taccataaat aaaaggcaca ttaatttaaa ataaaattaa
    14521 tgtgccttat ttataattct aatatattaa atttattttt taacttgtag tttaatatcc
    14581 tttttcttca attaattcat ataaataaag tatttaataa taaatgttcc ttatgacttt
    14641 aatattttaa accaagaaag ttaaagtttc tctcatataa taaattgaca cttatctatt
    14701 tcattctttt aagtgtacaa taagtcttct tctagcagta agagtaagta cttcctctac
    14761 ttatcacaaa ctataatttt tatctcattg gtatatatcc acgatactgt gagtatgcat
    14821 ccatagttgc tatatcacct aagaatactg atgcgatatt gaattgtggt tttaaatatg
    14881 ggaactcctc ttcttgtgcc cattctggtt ttttaataaa cgactttgtc tcttcaacca
    14941 cagctttata ccattcctct tccccttcaa accatagttc tgatacacgg tcaaaagcag
    15001 ttgctccata attaatcatt attttactac tgacaaaacg attaacatag caacagtttg
    15061 taaagtatgg cactacctca tcatagaacc atttttctcc ttcttcttta gagataccat
    15121 ctggatactt aatcataaat tgccaacgat agtttggtcc atcttgtaca gtacgtcctt
    15181 ttcctctaaa gtcttcttcc cagtttattg gaacaaaagc aaatataaat ggtgggcatc
    15241 cattatctcc acctactgca cgtcctgcat ctccactttc tgcattttca tgaacccttt
    15301 tattttctac atctggtata cacccttgcc aacgtagaac atccataggc atatattctg
    15361 tcataattct atttgccatc tcaggcatat gttcatctac aagccaataa tgttctgtta
    15421 gttgcatctt acgtgcacca aaacgctcac cttcatttgg tgtaggatat gcttgataaa
    15481 aggcatattt agtacaatat ggtccaaaag tgctaatact atctggaata tggtgacgat
    15541 ataaccagtc cattaactta ggtctatatt cttccttggc aagtcctaca tatattaaac
    15601 ttctcattat tttaaattca aaacccatag ccattctcct ataatcaaag tatattcaca
    15661 tgctttcaat ttcccttttt cttatcttta ctcaaaatat atattgtaaa caaatctaca
    15721 cctgcaccca cgtaccatac tcctactgcc atagctacag caatttgaat acaaatatct
    15781 tgtaaattaa taatattttc acctgtcata atagttagac ctatagccca accacaaagc
    15841 catgatggta ggaaaattat tttttcaaat aaggatgcaa ttacaactgc aaaaaaacct
    15901 aatatactaa gtgttaaaaa taattctagg ttttgattga aattcatata tcccataatt
    15961 aaaatggcta acactgccca taaatctcca caccaaaaac caaacattat ttttaatcca
    16021 tctttttgtt tattaccatt agttacatat aatcctgcac agattagtgc tacagctcca
    16081 gtttttattc ctatataagg tgctaatact gcccataaag gaggtagtaa agcaatcccc
    16141 atagacagtg ttaatatttc ttttttcttt ctatccattt cctatattgt aattagtagc
    16201 ttccctagct tgccttatct ccaatagctt aattgctatt tgatttacaa tcatagatac
    16261 tgcaaaataa cttaagtata atggtataaa tataacaata aatgctggag ttaatccaat
    16321 cttgtaatat gtaagtacca aaaaaataac tgctaaaata atttcaattt gtttaaaact
    16381 tggcttttca ttcatacaat aagcctccta taacatctaa catcccttaa aaaagagtct
    16441 tagtatttta attattatcc taatgaaact ccagcttgct tttctttagt taatggctct
    16501 ataaaattat aagatgtttt tctttcttta aattcttgtg ttttacgaac ttctgcataa
    16561 tgaccttcca ttttcttaat atcatcacca tgtataaact tacctggtaa agtatcatga
    16621 gcatgaccta attgctcttc tggaaaacta aatatagttt ttccacttat agtatctaac
    16681 tctccaacta taccacatag agcacaaatt gcctttttag tacctgactc aaggtagaat
    16741 aatctattgt ggcaatgtgg acatacacct tcttctcctt gatatgaagc tttttcaaaa
    16801 tcttttgctg cttcaactat atttaaacca atttgatgaa cttgaacaag ccttgtatca
    16861 tccattacta attcctttgc ccatgggaac caagcattat ctataacttt ccacattggt
    16921 atgagagcta gcatagcatg ttcacattga actcttgtac cccagtcaga acctcctatt
    16981 cccataaatg atattacttt atcattaagc attcttgagt ctggaatttt tcctcctata
    17041 gcttctgcaa cttttgttcc tataatgtta tttcctctat ctgctctggg tccaaaacgg
    17101 tccattatag tatggaataa tcctgatgcc ccttcttcaa aaatagggtc tgaaactact
    17161 ataccatctg catctttcat tttgtcaagc aaccattcaa aatcatcttt taatacacac
    17221 atactacctt tcccggataa taaagtttta acgcacgcac aacatccagt acaatgttta
    17281 atgttaatag atgacatttg aataaattct acttcagccc ctgcttcttt ggcaccttta
    17341 agtgcaactt tacacattgt gtcattattt ccattttttg ttccaaaaga aatacctagt
    17401 attttcattt tcaactctcc ctctttagta tttatttaat cttctttcat ttttaatata
    17461 tagaatccat catctaaagc atctatgatg atgtagcctc tatcatcaac gataagatct
    17521 tctgttactg ccaagcgtgg tcctgggaat cctgggaaat aagattcttc tggtgtttta
    17581 tttggatttg gtggtataaa atatgctagc tctttgatat aatatgggtc tgatacatca
    17641 taaaccctta gccctgcatg gaaataacag caatatactc tatctcctct ttgctctaac
    17701 catggcttat tatccattgg ttcatgaaga ttatgtggac caaatggccc tgcttttcct
    17761 aatcctgcaa cattaaagtt aggataaggg aaatcttttg gaacttcagg atatgggaat
    17821 tgagcaatta atgttggttt tgttgggtca ctaacatcaa ccatatgtat attattcata
    17881 gcttgaacat ctgtaatgtt atctggttta aagaactgga atctttctcc ctcattttga
    17941 acaacgacta aatctcttcc tggcaatggt aatgctgtat gagttcttgc acctgcaagt
    18001 ctactagaaa atgcaggcat aaatggcaat tcacctaagc atcttggtct tgttaaatct
    18061 tcaacatcta atacaactaa accagctcct ccataaccac aatatgcttt tccgtctctt
    18121 acaaatggag gtccatgaag ccatccttta tccataaatt ctgggcaatg aggtgctcct
    18181 gcatcaaaag ttctatttgg atatccatct gcatattggt ctggtctcca ccatttacct
    18241 atttccactg gattattagg atttattata tctatgaccc tataaatcag accttcaaag
    18301 ccaacacaat cacttgataa atgtacatat cttccaccat tgtacatgaa tctatgaaca
    18361 cccattacat gctttaagcc acaatcccaa tatcctaaaa acttaggatt taaagggtct
    18421 tcttttaaac tgtatattct aattcctgct tcacacttaa tatttgctaa tttagcttgg
    18481 tcaacaagtg ctcctggtcc acttccacaa ctcattgcta ctatcattaa atcatctgct
    18541 atttgaattt ttggagttgt tgttgtagga tactctttag ggtctaacat ttgaaaatgt
    18601 tttataaatt gtggattata agggtctgta atatcgctaa tcattactcc atttttttta
    18661 tttcctccaa aacagcatcc atataaatag tatttttcat ctcttttgta cagagccatt
    18721 tgaaacattc cacaagttcc atctaagtta ttaaaggaac aaacttcaag atttttaata
    18781 tatccgttat ttccgttgcc ttttgcataa aatttatctt ccattttttc tcccttctta
    18841 tttaaagtat tatcaattga tatactttca ttttatgaga caaacttact taaatccaat
    18901 atcgttttca ttggaatata taagttattc ttattaataa atttataaaa tctaatgtaa
    18961 agatatactt aaaacatata tttttataca aatattttct aatattcttt aaaagtaaat
    19021 tttcttatac tataataagc tttaattata gataaataag gtttacaaac aatcaagttt
    19081 aaataggggt gattatttga atactaaaca attagaatat tttatatctg tagcagaaaa
    19141 tctaagtttt accaaaacag cagaaaaatt ttatatttct caaactgctg ttacacagca
    19201 aataaaagct ttagaagaac aaataaatgt aactctattt acaagaagta aacgtcatgt
    19261 tgaactaaca ccagccggaa aagttttttt atctgaggca cgtactataa taaaaaatat
    19321 caatgatgct attgcaaaaa cacaacaatt tgcacatggt ttttttggaa ctttgagcat
    19381 tggaacctta ataggatatg aaaaaaacaa acttcaacaa tacttaaaag aattttctaa
    19441 tacataccct aatatatcta tggatatttg tactaatgaa ataaccgaac ttttaaactt
    19501 agtaaaaaat aattctatgg acctcgcttt tgttattaat ccagaaaatc aaccattaaa
    19561 agattttgaa tataaaactg tagaaagata ttctttggta gctcttttac ctcctggtca
    19621 tccactttac aatgaggatt ctatcgattt aattgaatta caacatgata aatttatttt
    19681 tgtaaaagaa acaggggatg agtatggaca aaagtcaatg atacaaaata gatatcgtga
    19741 agcggggttt gtacctaatg tggtacaacg ttcaaataat cttaatacta ttgaatcatt
    19801 ggtatcttct aacatgggaa tatccatttt accttcattt tgtgttacag atacaatgat
    19861 agaacgtgat attgcaatag tacctataaa agaagtagaa aatagaattg aagttgtggt
    19921 tgtatggaat aaaaaaaata caaatccagc attagaaaag ttcatttcta taatataaaa
    19981 ttaatcaata ttaactataa aaatagcatt aaccattttt atatggttaa tgctattttc
    20041 tactatttta ttatctaact tttaatttaa tatttataat cgttaaaaat aactctgttt
    20101 aacaaatata agcaagtcaa aagtgtgtct aaaaaagata aaaattttat tatatacaaa
    20161 tgtattatct caatatatct attttttcat tgttcatcag tctaagtaca tgacctgtgc
    20221 ttactaaaga attatcccca taagttgtat gagctaacac cgaactagca accccaaact
    20281 caactatatc ttcactcttc atattttcca aatatgaata tataattcct gccacatatc
    20341 catccccagc accaactctg tcatatattt ctaacttata ctccctactt tcaaaataac
    20401 catcatcatt tgatatatat ccctttataa atctattatt catatcatca aatcttctca
    20461 tagttccagc tacaatatct atatcatact cttttttaat caattctgaa ctagatttta
    20521 tatttagact atctatatta agtatcttat ttatatctct ataactagca aacagcccat
    20581 gacaatatgg tagtattttc ttataatcat ttactaaatc atcataagtc ctttctggat
    20641 ttaatgattg tctaaaatta aaatcaaaga atacttttat ccccttttca taagattttc
    20701 tagctagttc taaagcaatc tttttaactg aatctgtaag ttgcatagca ataccacaaa
    20761 tatggactat atccatttct tctaaacatt tatcaatatc ataatcttgt aatttactct
    20821 cacaaaatga actatttaac ctattcaaat aagtcacttc agaaggtctt tctccatacc
    20881 cattttcaag aaaatacact cctatatgat tccctccata tgtgataaag tcatctttta
    20941 ctccaagctt tcttatattt gcacttgatg ctttacctac attattatta ggcaactttg
    21001 tcaacatata ggtattgtat ccaaagtttt taagtccaga taatatattt aatcccgtgc
    21061 ctgtaattat ataatttagt tcattggttt gtgttaagaa cttgtagtca ggtactgaaa
    21121 gtctcatcat tatttcacca aaagctaata tattcataaa tctatcccaa taattctttc
    21181 attattttat aaagtactcc tatatcttcc actttcgtat cacctgtttc tttatcaatt
    21241 attgatgtat aaacatgagg tataactttt tctacaccag catcaagtgc aatttgaact
    21301 atctctttga agttatctaa gtctataccc cctgttggtt ctaaataaaa attatgcttt
    21361 gcacaagctt ttgcaacttc tatatattca tcctttgttt taagccctcc cattggaaaa
    21421 aacttaattg agcttcctcc catatctttc atcatagcta ttgctgtttc tataggaact
    21481 ataccatctt tttcttttga acttaatgga cctgtggata tttttacaaa tcctggttta
    21541 ccagttggag aaactaatcc atttattata ctatcatttt gaccaagcaa tgctctagat
    21601 gtagcgacac ctgtaaacac ttgatttata tgttgtggct gaagttcttt agaaatctga
    21661 gataccatat ttgactgatt tgggtctcca gctccaagac ccacagatat tgcattatct
    21721 atttcctttg agtatttttt catatcttca atagctgctt ctacagtgct ataattcttt
    21781 gatagtactc ctactagtac atgtccatca caggcatcat atacttcttt agcatttttt
    21841 atgcttcctg caagaacatt taaacaaact ctatttttat aatattgtgg tatacaattc
    21901 atatttatcc cctccattgg taaaattcaa tttattaaat cattataatg tcacttactc
    21961 tacactctaa ctttctacta tattttttat cctattataa atattatcta aatcacttcc
    22021 agttacactt cttatatcaa tctcaatctt gccttcatta gccttatagt ctctagtgta
    22081 tatagctatc tctccacttc taagttcatt tataatttgt tgtgttgact tatttattat
    22141 atcttcatta aagttaattt ctccacgaag tatttctcta cctgctgaat ctctagttat
    22201 actagcactc actcccttta tagtatttat cttgtctata aatggattta gcttgtcctc
    22261 catttctttt aaagttactt tatcattact tatatatctc tcaattgcac atgttagacc
    22321 tacaatattt tcttttccta ttttcatagc tcttcctata ccctttgatt gaagctttac
    22381 attatctatg tattttttct tacctattac taaccccgag ctaggacctt ctatagcctt
    22441 agcaccactg tatatcacta ggtctgctcc caattcataa tatacattta aatcttcttc
    22501 tgctgctgca tctactatca aaggtaaatt atattcttta gcaacttgta ctgcatctaa
    22561 tacacttagc atactctttt gaacacaatg atgagattta atatacataa ttgctgctgt
    22621 attttcattt atctctctta ttatatgttc cttgttacac atattagcat agccggcctc
    22681 aacaacttct cctcctccaa gtcttatcat tacttctact ggaactccat aatcaacatt
    22741 atgccccttt ggaattatta tttccctttt aacttgtttc ttaggattat ttacattgta
    22801 aactagactc atatcatctt ttgctatcat tgctcccaca ctctgtgcaa tcccagcaga
    22861 tgcactagaa actattagtg cactttcaca ccctaataaa tttgctatat attcacctgt
    22921 tttatttaca agttcttcta ttataaggaa atttttacac ccttcaccta ttgcttcaat
    22981 aacttcctta tctactgttg atactcctag tatagtcatt ttgcctgaag catttataac
    23041 acgtcttaca gagtattttt caaatatact actcatacaa tagtttctcc cttctactca
    23101 ttctcttcta tagagttgcc tatatttaaa tattctccat tgactactac tgctcttggc
    23161 tttatagacg tacttgtgat tactgactta ttcaaagaat cttgaagttc tttttcttca
    23221 ttaaccacat caaatatagt taaatcagca tcatatcctt cttttattaa acctttattt
    23281 tttaaactta ttgcatctgc tgcattttta gtaactttat ctattatatc ttcaagtgaa
    23341 tagcccatat atataaactt ttccattgtt gtagatagat tatacactgg accatttatt
    23401 ctatttttaa tatatatatc tgtacttata gtgtctggaa aaattcctgc atccttagat
    23461 ttcattgcaa tatccatact aaaactttcg gttccatgac ctacatcaag aattatacct
    23521 ctttctttag cttctatgat ttccttttta acctcattgc ctctaagtat tccatttggc
    23581 tttccattat atatatgggt taatatatct cctttttcca tataatcaaa tatatcctct
    23641 atcgttggag gagaactccc aaagtgaacc atcataggta gattaagttc attttttatt
    23701 cttttagcta cttttaaagg ttcaacatca tttgaaatta caacactttt actcattcta
    23761 gctttgattc caacaacaaa atctttgtat tttctaactg catttttaag ttcatattca
    23821 tcaatattca tcatatttga aagttcatct tgagatgtta ttccctgctt agcaatattt
    23881 attaaagcat atacattggt cttatacttt ttagtatctt cataaaattc acctatatct
    23941 aaagctcctg ttgttcctgc atctactaca gtagtaactc cactttttat tccaacctca
    24001 tctggataat ccttataaag gtctagtctt tcgtagcagt gtgtatgtat atctatccaa
    24061 cccgcactaa taaatttctc tccatctaag tttataattt gttttgcctg atacttagaa
    24121 tcatctatat gattttttac ttctattatt ttatcgtcta atatagctat atcaattata
    24181 cttttatcta ctgtttttcc acattttatt aatatgtcta tcttcacttt actctccctc
    24241 taaaataatc ttaaatttac tccaaggttc aataaagtct aaaagtatta tttcgtcttc
    24301 ctttattcta cccaccttat tttttctttc atctgtacaa tcttgaagta tcaactgtaa
    24361 ttccccttta tactttccaa agtcatcatt accaatcact acatctcctc ttttaaagat
    24421 gttcttatta tcatgtttag ggaaattttc tttagcatat ttttttctta cttctgtaga
    24481 tcttgctacc tgattagtta tatctcctct tctaaagtgt tgctcattta ccattattat
    24541 cttttctact tcagaagttt tttctttagc tactacatta aatacaactt ggtattcatt
    24601 tacttcagac attcttttta attcatcttc acttgcatat gcatttccta caattacatc
    24661 atcaatcaat cctgttgcga ataagtgctt agttgctgta gcaatatcta agtttctatg
    24721 catttcaagc gtacacagtc catcatttat attccaaggt ccgattgttg cgtattttga
    24781 attgacaaaa gcagcagttc ttattccata ctttttaaat ctatttgagc atcttacaaa
    24841 aaaatcatat ggcaagcctg tacccctttg aggataaaaa ttgtgacaac catatatatt
    24901 atttacattt ggcttgtaag taattatatt atccaagtat gctacatcat tactcatatt
    24961 tagttccact tttaaatcat atggattgaa agaaatcata gcttctttac ttccatcata
    25021 tccatagtca agccttattc cactagcatt taactcacta aagaatgtta aatcatcata
    25081 agatatttgt aatatatcaa atatgtttgg agctatatca aggattactt caaatcctaa
    25141 ttctcttgca taggttataa tctctttaaa atgtttttta acttcttcct taggtcttgt
    25201 aattgaaagc atgcacataa atattctttt aaatccgtat tttgaaacca tttttatata
    25261 tgccttatca tcttccatgt tactatgctc tggatatact gatattccta atcttctttc
    25321 catctttctc ctcctcaaat tacattattt aaaatatata aaattaaagg caaaggtcat
    25381 tcctcctacc ttcaccctta ataacttata agctttttct atattttata tagtacaaaa
    25441 cctctatatc tatgtatttt tataaatttt cactcatttc ttcttgttct ctttgtaatt
    25501 gttgtttttc aaaaactttg aagaatggat aataaacagc aaatgatatc acaaagttga
    25561 ttaatactaa gatacctgct agtatactcc agttagtact cattactgct gctattggtg
    25621 ctggcattgc aaatggtaat cttgctacca tcataggtat aacccctgta atagtaagaa
    25681 catatgatag aattgtcatt attataggtc caattataaa tggtatccct agtattggat
    25741 tcataactat tggtgctcca aatattatag gttcatttat gttgaataac cctggtaaaa
    25801 atcctaatct acctaaactt tttagatatt gtgacttaga aaacataaac attacaacta
    25861 atgcaagagt agcccctgct ccccctatcc atacatacca ctgtaaaaat tgttctgtaa
    25921 atatgtttgg cattgcatga gctgatactc cttctgtgaa tgcagttata ttttctgcta
    25981 tagacatgtc ccatataggt cttattactg gccccattat agctggacca tgtatcccaa
    26041 gtacccagaa taaagttatt aggaatactg tcagtagccc accacctaaa ctatttcctg
    26101 ataagaatcc ttttaaaggt gataacatag cgcttagtgc aacacttata tcaaatccta
    26161 aaatatgtct tattacccag aataacatta aaattactgc tgtcggtaat aatgcagtaa
    26221 atgaatttga aacctctact ggaactcctg ctggcatctt aattgtaata ttcttttcct
    26281 tcataaaacg atatatttca actgtaatta atgatgttac gatagctcca aataatgatg
    26341 gcgaattaag ttttgccata tccatccatc taccagcttc tataactcca cctactggtt
    26401 caactacttg agttggaact attgtagaaa ctaaaaatgc cataactgca agcataccaa
    26461 cacctgttgt atctaaatcg tagcttttag caagtgatga tgctatccca aaagtagcat
    26521 atagagataa aaatcctaca gtatatctaa atggtatatc taatgttgct ttaaatggag
    26581 ctataaaatc catataagca tcaataggta tgtttaataa tattgtgaaa aatgacccca
    26641 ctattgttag tggtaatgtt gctattatac ctgctcttac agctctcata tgtctctgat
    26701 tacttatctt cgctgctaca ggcataatct tgcgctctaa aaaacttgcg aatttttcca
    26761 tagttatccc cctgtattat tgatattatc ctttatttat aatgcaataa cgatttcata
    26821 acataaacta atttttatgt aattgataat atcacttatt tattaaaaat attataacat
    26881 tataatgttt ggttgtaaac attataatgt tataatattt ttcgttttta caactaaaca
    26941 aaaatcctga gcatattatc atttcaataa actcaggatt cttattatat ttcgtattta
    27001 attacgtagt tttgtacttc tgtattatat cttgcaaatg atagctcaac tatattatca
    27061 ttctcatcat aggtctttct ttttcttcct aataatatgt ctttagctag atttagagcc
    27121 tttgatacct ctagttctga ttcttctata aaaaattcat cttcaaatct agttatattt
    27181 atatttctcc tatataagta catatataaa gaactttcat tattaaaact ttcaacagtt
    27241 agattttcat caaaaggaat ataatgagta aaaaatatat atggcttact atctaagtaa
    27301 tacattctag taatcttgct acatttttct ccaaatatac taaatacttg tgaatttgta
    27361 tcatttatta ttttttcaat tgaaatagtc tttttagata acttatgacc tttagataaa
    27421 agtatacttg aaaaagactc tgcttttgat agcttattaa aaagactatt acttattact
    27481 tttgtccctt ttccactttt tttctctaca tacccttctg attctaatat ctcaatagct
    27541 tttctaatag taattttact gactccaaat tcttgctcta attctctttc tgttggaata
    27601 ttttcatcaa ttttatattc gttatttaat attttttgct ttaatatatc tacaacttgt
    27661 atatataatg gttgatttct taacatgatt cacctcttaa attatatgtt ataatattat
    27721 aacatataat cttatattat taataataaa tatttattaa ctttaaacta aatataaaaa
    27781 gagtcctcaa aataatccta atgtattact cactaaatat tgaatcaata atctaacaat
    27841 tagtgtgcta cacataatgt atcttttaag aactctctta atttattctc tttaattttt
    27901 atttatcttg taatttcaaa tgtaaatcaa cgatttcttc agctaactgt tgaaaattca
    27961 taccattcat taggtggtcc tgtgcatgta taagtatgac acttatttca gtcctgtttc
    28021 cactagcttc gttttgtatt aattctgttt gatatctatg tgctttattt ataacttcct
    28081 tagactcttt taataattct tttgcttttt ctatatttcc attcttagcc tcttttattg
    28141 cttcaaaagc taacccttta ccttcacctg cataacctat tatattaaaa gatatttcta
    28201 taatcttttc atccatcctt tgattctccc aatttatata ttaaaaatac attataattt
    28261 atattactta gtgttgttaa gttctatcgc tctatttaaa acagcttctc cattaactgt
    28321 tccatagtgc atcatattta taacctctac tggtatatta tatggcttag caatttcttg
    28381 agccttaggt aatacatatc taacttgtgg tcctagtaat aatacatcac atttttctat
    28441 ttcattttgt atagttgatt ctggtattgc ccaaatttgc gactctatac cattttcttt
    28501 agctgcactt tccattttag taactaataa acttgtagac attcctgctg aacaggccaa
    28561 cattattttt atcatatatt ttcctccaaa tttttattaa caactatata aacattataa
    28621 tgttataata ttttatttaa ttctacctta aatgcaatat actttcaata cttttttgtt
    28681 attttaatat tattaagtgt caaattagca aaaaccgtat catttattta tgatacggtt
    28741 ctccattatt tatcctataa gccctataaa tctgttctaa caatatcaat ctcatcatct
    28801 gatgaggaaa agtcatctta gaaaaggata acctataatc agcccttcct aaaacctcat
    28861 cagaaagccc taaagaacct cctattataa aacagagact actattacct ctaactgcta
    28921 aatcactcat agtcttagca agttcttcag aggataaacc cttaccatca atagccaaga
    28981 ctataacata actattatgc tttatcttcc ctaaaatttt ctttccttct ttgtctttta
    29041 taatttccat ttccttaaca ctcaaattct ctggagcttt ttcatcatcc aactctataa
    29101 tttctaactt acaatactta gaaagccttt ttttgaactc atcaatacct aattttaaat
    29161 atttttcttt aattttacct actactacta tacttatatt catatatttc tcctaaactt
    29221 aattttatta caaatctatg cattactaga atttacaagc tcttataaaa ttatatctct
    29281 ttttcttgat ttgcatattc tacaaactct tcaaaaggta ttgctttgct ataataatat
    29341 ccttgatata tttcacatcc taaaccatat aacttatccc tttggtctat attttcaaca
    29401 tactcaacaa ctatatcaaa atctaaattt tttgaaagat ttacaatagt cattataatt
    29461 tcactacttc ttttatttgt aagtatctct ttaactaatg aaccatccaa tttaactata
    29521 tcaaagttat tattttgaag atatataaga gagctatgcc ccatcccaaa atcatccata
    29581 attaacttta ttcccaagtt atgtatcgca ttgatacgtt caataattat ttgtgaacct
    29641 gataatgcaa tttgctctgt tatttctata cctaacatat taggatttat atcattacta
    29701 ctaatgatat tttttatttg ctccggtaac ttagggtcat caagttggtc tggagatata
    29761 tttacagaga acttaacagg atttctcaat agcttttcac tcttctttaa atcaatacat
    29821 gctttattaa taatatactc tccaagctca tctaagaacc cttcttcttt agccaaaatt
    29881 ataactagtg gtggataaat gaaccctcct attgaatgtt tccatctaag taatccctct
    29941 gctcccacaa catttccatt ataattgact tgtggttgat aaaaaagttc tatttctcct
    30001 cttttcatag caaacttcaa gtctgatgcc aacatttttg aaattgaacc tattttacca
    30061 ttatggttta gaagattagg ctgttcacct gttaattcat acctttttaa taagtttgtt
    30121 aaattttcta tattattctt aaacatatat gtatatctat tttgcgacat ttttataaac
    30181 ggaatataaa tcataacacc aacacataaa ttaaatactt gcaacaaact acctcttatt
    30241 gaacctgtag acatatatcc acttaaaaat ataggtgagg tccattctac agctgaaact
    30301 gtacatggta caatacctgt agacatagcc acataacttg taatggttaa gattatagga
    30361 gtaattacaa aaggtacaaa cataatatag ttaaaaacaa ttggtatccc gaaaactagc
    30421 atctcattga tattaaaaag agctggtatt gaagcaattt tagctagctt tcttacattt
    30481 atacgttttt cactaagaaa aattgcaatt actaaacata gtaaagagcc acatcctccc
    30541 aataaaacca ttgtatcaaa aaaggtctta gtaaaaattt ctgttggtaa ttgattatta
    30601 ttaactaaat ttatgttaat tgccatacta ttttcaaata aattttttgc gacggtatct
    30661 aatacattac taccatgaat cccaaaaaac cacataaagt gcattaaaaa aataaacaac
    30721 agagcactca taaggtttgt tcccatttta ttaaaaacat ttgaaaataa attataaaat
    30781 atatcttgaa agtttgaact gccaattaga cttatcatta tcactctaaa aatagaaaac
    30841 aaaaatacta ctattatgaa aggaactata gccgaaacta ccatattaaa gtctgcatca
    30901 gccccttctg tatgaaactt tacacttgta acaaaagatt ctgtaagttt tacaaacaat
    30961 acagaagatg tcatagaaac aattatagat gtaaataacc actttgtctt aaatatttct
    31021 atataagaat taagctcatt accatgtgaa aatactaaaa aagagcacat tgcaactata
    31081 ggaacaagta ctgtatactt gctcccatat atttttccat aactatagct tattgttaat
    31141 aatattatta atgaaataat tcctaaagta gaatcattta taaagttaag aatatttgta
    31201 acctctccat taaacaagcc tgataaatat tcttgataag cctttattgg aagattcaaa
    31261 attacaagag ccgttgaacc agtcataata gcaggtattg ctaaaatcat accctgcttt
    31321 atagagtata aaattttatt atcctcaaca ttttcaaaat ttctctttag caaagttatt
    31381 cacctctact tcatctcata aacattagaa acatcatttc gtgatagtac atctagtttt
    31441 acatcttgtc caactactat atcatttgat gctaatactc cttttgaagt ctcatatgct
    31501 aactcaggaa aattattttc tttacttaaa tgtgctaata atatagattc tgtaccttca
    31561 ttaataagtt caacgcagaa attagctgca tcttcattgg ataaatgacc agtacttgac
    31621 attactcttt tctttaaagc atatgtatat gaacccatca taagcatatt agggtcataa
    31681 tttgattcca atactactaa tttagattta tatagatgtt tttttatatt ttgagttata
    31741 catcctatat cagtagctat agacattttt tcatcactct ctgtataaaa attatatcca
    31801 actggatcag acgcatcatg ttctattgaa aaaggtctca ctatgatatc acctaatgag
    31861 taggttttat cattttcaaa tactttcata tttttatctt tgatgtcacc taatttgtcc
    31921 ttcatagaac accatgtttt tatattagca aatactggta tatcgaactt tctggataat
    31981 atacctgctc cttttatatg gtcaacatgc tcatgtgtta taaatattcc ttttaattta
    32041 tctgcatcta caccaatgtc tttaagacct gtagttattc tcttgccact taatcctgca
    32101 tctactagta tattagtatt tttataccct atatagtggc aattcccact actaccactc
    32161 cctactgaac aatactttag cattccttca cctctttttc tgccttataa attatattat
    32221 accaaaaaaa cagagtatta aataaaattt aatactctgc acaaaattta cttacattca
    32281 ctatttactt acattcactt ataatcttat ttatatctac acctttacta ttccatagtt
    32341 ccacaaaagc tcttccatta gcatttttat gcttatattt attatagtat tcatttaaag
    32401 tctcaaagaa tactttatct ccaacttctt ttcttatttc attaaatgct attgcgcctt
    32461 ttgtataaac attcaagctg tattcagtag aatttttata ctcatttgct ggcttaaata
    32521 tattttcact caaataactt tttgtctgta tctccatagt ttttataagc ttgtttgcca
    32581 tatctttacc atacttttgt tcaaaataaa ctattgtaga atattcagta agtgcttcat
    32641 ctaaccaggg ttcacttatt tcatcatttc caacagcaga ataccaccat tgatgtgctg
    32701 tctcatgggc aataacatat tctagtaaaa attcattttt ttcattatat aaactttggt
    32761 ctatcataac aagcataggg tattccatcc cccctataaa aaagtcactt gctacaacag
    32821 aaaattcttt gtaaggatac tttccaaata attcactaaa tatatttatt gaatcttttg
    32881 ccacttcagt tgctttttta gaaagctttt tatttaaatt atatgtattg atagctactc
    32941 cctttgaatc tactttgttt acatcaaatt tactacttag tataaatgca aaatctctaa
    33001 cgttttcagc ttctatttca tatagcattt tcttattatc atgtttatca tcaataagtt
    33061 ttcctgtatg agcaatttta tatttatttg gaatcaatag tttcacatga aaatcgctag
    33121 tctcactata aaatgggtcg ccaatagttt cataactctt taaattccat cccctttcat
    33181 catttacaca agctatagga aaccaattag taacatttat agtattttct ccatatccaa
    33241 atcttccaat tgaatttggt atttttacgt tatattttat atctaatgac attctttcat
    33301 ttggttttaa ctctttccct aattctattt ctaatatgtc atttttgtct cctttaattt
    33361 tatactccat tttactatta ttatttaata tattctttat atctatatat ccttcgttaa
    33421 atccattagg ataagccctt gtcatttcac ttttttcaaa aggagcaaaa tctttcttgg
    33481 aaaaagcatt cggatatata tggaaatata ttttatctat gtttgatttt gtattattta
    33541 tgtagtcaac attctgattg cacattaacc tcttagtctc atcatcaaat ataacatcca
    33601 tattatactg atttattttt gaattagttt tttctttgta tgtttctaaa ctgtcatcct
    33661 tttctataaa gcaacctatt attaatacca taaatagaat cgctactgat aatgttcttc
    33721 ttactttttg gttgaatttt ataaccaaaa tatctcctcc ttttcaaaat gaatactttt
    33781 agaaaatatt tacatgctaa tcttgtgacc ttttatttgt ttacagacaa atataatata
    33841 aaaaatgata taatttaatc tgaggtgata aaatgttttt taaagaaagc aatgaaatag
    33901 atttaggtga aatcactatt cccaatatct ttatagatat atttatgcca atggcggacg
    33961 ggttatacgt aaaagtttat cttttaggat atagacaagc atgtgatatt acttctaatc
    34021 ctaagttcga caataattca atagccaaaa acctaaatat accattgtct gatgtgttat
    34081 ctgcttggaa attctgggaa gaaaagaaga ttataaaaat acatgacaat ggagaatatg
    34141 ataattttaa ttattcaata gaatttttag atttaaaaaa tttctatatt gagaatatat
    34201 taagcaataa ttcatctata aaatctaata cagataaggt tgtatctact tctgaaaatc
    34261 caagtattag aaaaatgttt aattctataa ataaaatagt tgggcgatat ctcgacccaa
    34321 gtgaaaagat gagtattatg gatataatga ataaatacaa tatgagcccc gatatgatac
    34381 tttgcgcata tgaatacgtc aaagataaaa ctggtacatc aaagccagtt aaatacattg
    34441 aaggcattat tagaaattgg tatgattcaa atttatatac gccaaaagat gttgaagaaa
    34501 gctttttggt aagaagtgaa cgatatattt tatataaaac gattttcaat gaattgggat
    34561 tttcaagaca accatcaaaa tcagaaaaag aacttatgga cacctggttt gataaattta
    34621 atatggatat agatttgata ataaatgcat gttctaagtc taagaatatt tcaaacccaa
    34681 gtatatctta tataaatggt ataattaaaa attggaatga aaaaaatata aaaaatttaa
    34741 atgatttaaa gcaaaaagag gaagaacgta tagttaagga gaatattaat aagaagcaga
    34801 ttaatactac tcaaaacaac aatacttaca aaaaaactaa gtttcataat ttcaacgaaa
    34861 cattcactca gtatacctct gatgagctcg atgagattat taaaaaaagt caaaaagaaa
    34921 agtttaagta ggtgattaaa tgaatgagga taaaataaga aaaatacttg ctaaatatgc
    34981 taagagaaga gatgataatg aactcttatt agaacataga aaaaatgagg tatataatag
    35041 aatccctgag ataaaatcta tagatgatga aatttctaaa ataggtctca gtttagctaa
    35101 gatagtactt ttaaatccca aatctaaaga tgaaattgta aagaagacta aagagaatat
    35161 agagtcttta aaagtaaaga aagaaaaact tttatctgaa agtaacatac ctcttgatta
    35221 cttggaaata aagtttcaat gtatttcttg taaagataaa ggatttttac ctaatggaga
    35281 aaaatgttct tgcctcaaac aagaaattgt aaatgaagct tataaaatgt ctaatttaga
    35341 taggatttta agtcaggaaa atttttcgaa ttttaacttg aatatatttt ctcctaagaa
    35401 aggctctgat ggggaaatat ctcctagaga aaatatgcta aataatttaa gtatctgcga
    35461 aaattttgtt catgatttta agaaggataa tagtgaaaat ctactgtttt atggttctac
    35521 tggactagga aagacctata tgtgcaattg tattgcaaag gaattgttag ataaaggaaa
    35581 tgttgtaatc tatcaaactt cttttagaat tttagatatt cttgaagatt ataaatttag
    35641 aagagacaca aataaccaaa taagtgaaga taattataaa aacttgtttg attgtgattt
    35701 attaataata gatgaccttg gaactgaact taataattct tttactagtg gggaaatctt
    35761 caatattgta aatacaagac ttgttgctgg taaaaaaatc attatttcaa ccaacttaac
    35821 accatctcaa ataggaaata cttacactca aagaactctc tctagaatct tagataagtt
    35881 taggatttta gaatttactg gagatgattt gagatgggaa agatttaaat aaaatagccc
    35941 ttaaaaaaca tttttttatt tttaaataga gaggtaagag caaacttgct tctctgttta
    36001 aaacttatgc cacataaatt aaaacatatt ttttattttt ctatttacat atctaatgta
    36061 tcttaatcta acaaatcatt
//
LOCUS       DACTGW010000010        35644 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.010, whole genome shotgun sequence.
ACCESSION   DACTGW010000010 DACTGW010000000
VERSION     DACTGW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.010"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(121..894)
                     /locus_tag="I9573_000419"
     CDS             complement(121..894)
                     /locus_tag="I9573_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HAT4795057.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1301)
                     /locus_tag="I9573_000420"
     CDS             complement(894..1301)
                     /locus_tag="I9573_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAT4795058.1"
                     /translation="MACETIKMEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVA
                     VSEACTNAIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLG
                     LFIIKTLMDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="I9573_000421"
     CDS             complement(1305..1622)
                     /locus_tag="I9573_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HAT4795059.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVKKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="I9573_000422"
     CDS             complement(1634..1894)
                     /locus_tag="I9573_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAT4795060.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="I9573_000423"
     CDS             complement(1894..2190)
                     /locus_tag="I9573_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAT4795061.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="I9573_000424"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="I9573_000424"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAT4795062.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDIAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKIGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKNEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="I9573_000425"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="I9573_000425"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAT4795063.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="I9573_000426"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="I9573_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAT4795064.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="I9573_000427"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="I9573_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAT4795065.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVKIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="I9573_000428"
     CDS             complement(8083..9189)
                     /locus_tag="I9573_000428"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAT4795066.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10750)
                     /gene="dnaA"
                     /locus_tag="I9573_000429"
     CDS             complement(9431..10750)
                     /gene="dnaA"
                     /locus_tag="I9573_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAT4795067.1"
                     /translation="MDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILLA
                     PSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYPN
                     LNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHHI
                     VSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERTQ
                     EEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIAI
                     LRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEAL
                     KDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTDL
                     SLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="I9573_000430"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="I9573_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAT4795068.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="I9573_000431"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="I9573_000431"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAT4795069.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="I9573_000432"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="I9573_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAT4795070.1"
                     /translation="MNKIIYILKEISRYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="I9573_000433"
     CDS             12283..12990
                     /locus_tag="I9573_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HAT4795071.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="I9573_000434"
     CDS             12959..13588
                     /locus_tag="I9573_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HAT4795072.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="I9573_000435"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="I9573_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAT4795073.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGERALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNILDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15174..17069
                     /gene="mnmG"
                     /locus_tag="I9573_000436"
     CDS             15174..17069
                     /gene="mnmG"
                     /locus_tag="I9573_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAT4795074.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVDKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQVRR
                     TRGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="I9573_000437"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="I9573_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAT4795075.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGRPAKNPIK"
     gene            17956..18741
                     /gene="noc"
                     /locus_tag="I9573_000438"
     CDS             17956..18741
                     /gene="noc"
                     /locus_tag="I9573_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HAT4795076.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18876..19649
                     /locus_tag="I9573_000439"
     CDS             18876..19649
                     /locus_tag="I9573_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAT4795077.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19651..20514
                     /locus_tag="I9573_000440"
     CDS             19651..20514
                     /locus_tag="I9573_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAT4795078.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20547..21689
                     /locus_tag="I9573_000441"
     CDS             20547..21689
                     /locus_tag="I9573_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAT4795079.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVSDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21823..22398)
                     /locus_tag="I9573_000442"
     CDS             complement(21823..22398)
                     /locus_tag="I9573_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HAT4795080.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDTSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22658..23950
                     /locus_tag="I9573_000443"
     CDS             22658..23950
                     /locus_tag="I9573_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAT4795081.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24055..24657
                     /gene="yedF"
                     /locus_tag="I9573_000444"
     CDS             24055..24657
                     /gene="yedF"
                     /locus_tag="I9573_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAT4795082.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24730..24993
                     /locus_tag="I9573_000445"
     CDS             24730..24993
                     /locus_tag="I9573_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HAT4795083.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            25008..25193
                     /locus_tag="I9573_000446"
     CDS             25008..25193
                     /locus_tag="I9573_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAT4795084.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25296..26492
                     /locus_tag="I9573_000447"
     CDS             25296..26492
                     /locus_tag="I9573_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAT4795085.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLEKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26706..26984
                     /locus_tag="I9573_000448"
     CDS             26706..26984
                     /locus_tag="I9573_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAT4795086.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27021..27455
                     /gene="ssb"
                     /locus_tag="I9573_000449"
     CDS             27021..27455
                     /gene="ssb"
                     /locus_tag="I9573_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAT4795087.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27474..27701
                     /gene="rpsR"
                     /locus_tag="I9573_000450"
     CDS             27474..27701
                     /gene="rpsR"
                     /locus_tag="I9573_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAT4795088.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27844..28164
                     /locus_tag="I9573_000451"
     CDS             27844..28164
                     /locus_tag="I9573_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795089.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28172..29128
                     /locus_tag="I9573_000452"
     CDS             28172..29128
                     /locus_tag="I9573_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HAT4795090.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29139..31133
                     /locus_tag="I9573_000453"
     CDS             29139..31133
                     /locus_tag="I9573_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAT4795091.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31134..31583
                     /locus_tag="I9573_000454"
     CDS             31134..31583
                     /locus_tag="I9573_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAT4795092.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31602..32930
                     /gene="dnaB"
                     /locus_tag="I9573_000455"
     CDS             31602..32930
                     /gene="dnaB"
                     /locus_tag="I9573_000455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAT4795093.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33135..33443
                     /locus_tag="I9573_000456"
     CDS             33135..33443
                     /locus_tag="I9573_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795094.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33525..34814
                     /locus_tag="I9573_000457"
     CDS             33525..34814
                     /locus_tag="I9573_000457"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAT4795095.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNKIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35140..35215
                     /locus_tag="I9573_000458"
     tRNA            35140..35215
                     /locus_tag="I9573_000458"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35173..35175,aa:Lys,seq:ttt)
     gene            35222..35296
                     /locus_tag="I9573_000459"
     tRNA            35222..35296
                     /locus_tag="I9573_000459"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35255..35257,aa:Glu,seq:ttc)
     gene            35301..35376
                     /locus_tag="I9573_000460"
     tRNA            35301..35376
                     /locus_tag="I9573_000460"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35334..35336,aa:Val,seq:tac)
     gene            35410..35484
                     /locus_tag="I9573_000461"
     tRNA            35410..35484
                     /locus_tag="I9573_000461"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35442..35444,aa:Thr,seq:tgt)
BASE COUNT        13456 a         4600 c         5664 g        11924 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgtcgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttttta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 gtccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt cattctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatttctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctat cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagtt cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 atatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaaattt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccatctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcgt gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggtaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcttcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccccttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatgtata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt ttaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtgtt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtgt ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcgt taaacccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aactgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag aatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctaagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctattg ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttgctag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaggta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaaaaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga cttttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac ccaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa acctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa agagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt cgggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atattggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaac tcctatacag gagaagatgt tatagaaatt aattgtcatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 gggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga gattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataaggg atactgatga tatcgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaggaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaatttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggt ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gtcttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggcg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacct cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 tcgagaactt atttagtgca gggcaattta atggaacttc tggatatgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccgtttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagataa agaatttgaa agattaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaagggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ttagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatcggg tgtttctcca gctgatatat cagttttact tatataccta gaacaagtca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgaatgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaag actatagaga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaggtgat ggaaacacca gttaagtacc ctagaaaagc tggtagacca
    17761 gctaaaaatc ctattaagta atgaaaaaag taatgaaaaa attattattg gtaagcttaa
    17821 ttatttctta ggaaataatt aagcttaccg taaaaaaaca aaaataaagg aattattaaa
    17881 gtagatgcag aaataaattt gtatgtacat atagagaaca aaaataattt atatctaaaa
    17941 taagagaggt atgttatgga agataaaaga gttatggaga tacctataga agatgtagtt
    18001 ccaaatccat atcaaccaag aaaaatattt tcacaagttt cattggagga attgagcaat
    18061 tctataaagg tatatggtat aattcaacct ataacagtta gagcaaaaga tggcaaatat
    18121 gaactaattg caggagaaag aagattaaga gcagctaaat tagcagagct taaaaccata
    18181 ccagcaatta taaataatat gaatgatgaa tcttctgctg ttctagcgtt attagaaaat
    18241 ttgcaacgag aagacttaaa tttcattgaa gaagcaattg gttatgaaaa cttaataaaa
    18301 gaacatgctt ttactcaaca gcaattagca gaaaaattgg gcaaaaatca atctactgta
    18361 gcaaataagc ttagaatatt aaagcttcca aacgatataa aaatgaagct aatagagaat
    18421 aatttaacag aaagacatgc tagagcattt cttaaactac caagtgaaga tttaatgcaa
    18481 agtgtgttag acaaagtcat aaaaaatgaa ttgactgtta aaaaaacaga aaaattgata
    18541 caagatgtat tagaagaaac taaagttcaa gaagagcctg ataaaaaaca gaatataaag
    18601 ggtgctatga gtataagaat ttacataaat actataaagc aggcctttga tgctatatca
    18661 aatacaggaa ttgatgctaa gtataatgaa atagataagg gagattatat ggaagttgtt
    18721 gtgaaaatcc ctaaaaaata gagattactg cttaccagta atcttttttt gtaatataag
    18781 ccttgtaata taaattgatt tatagacatt atttatactt aaaaaacacc tattcaacat
    18841 ttaaaattaa tttaattctt aaagggggga acatcatggg caaagttata gctgtattca
    18901 atcaaaaggg tggagtcggg aaaactacaa caaatgtaaa tttaagtgct agcttaggaa
    18961 ctttgggaaa gaaaatatta gtattagatt tagacccaca aggaaataca actagtggat
    19021 atggtattaa taaaaacgaa gtagaaaata caatctatga aattatgtta gatgggttac
    19081 atattaaaga agcaataata agtactgaat ttgagaatgt agatgtagtt ccatcagcta
    19141 ctgaactatc aggtgcagag atagagctta catctaaaac taatagagag tatattttaa
    19201 aaaattccat aaaagcggta atagatgaat atgattatat atttttagat tgtccaccct
    19261 cactggggat gcttacaata aattgtttaa ctgctgtaga tagtgtttta attccaatac
    19321 aatgtgaata ttatgcttta gaaggtgtta gtcaattaat ggagactata aaattagtaa
    19381 aatcaagatt aaatgctgat atagaaattc aaggtgttgt tttaagtatg tttgatggaa
    19441 gagctaattt atcaatacaa gtagttgaag aagttaagaa atactttaaa ggtagtgtat
    19501 atacaacttt aattccaaga aatgtaagac ttgcagaagc tccaagtcat ggaaaacctg
    19561 taatttatta tgataaaaga tgtagagggt ctgtagctta tttggagcta gcagaagaat
    19621 ttatagattt agaagaggag gaatggtagt atggagaata agtctaaaag aagtaataga
    19681 ttaggtagag gtcttagtgc attgatacca gaaattaaag gagaaacttc agaaaaagaa
    19741 atagtcaaca tagatataga caaaatatat ccaaacgaag ttcaacctag aaagcaattt
    19801 gatgaagaaa agataaaagt attgtcggac tctataaaaa attatggtgt tttacagcca
    19861 atagtggtta aaatggatga aaataataaa tatatgataa tagctggaga aagaagattt
    19921 agagcatcaa aacttgcaaa taaaaatcaa atacctgcta taattaaaga tatagatatg
    19981 aaagatataa tggagatagc cttaattgaa aacttacaga gggaagattt aaattcaata
    20041 gaagaggctc tagcatataa gagtttaata gagcattaca atgtaactca ggaagaaata
    20101 tcagaagctg ttgggaagag tagacctcat ataactaata cactaagact tttaaatctt
    20161 ggacaagatg ttatagaaat gatagacagt ggtagaatta cagcaggcca tggtaaggct
    20221 ttattgagaa ttgcagataa agatttgcaa cttcaaatag cgaaaaagat agaagaagaa
    20281 gaactttctg taagagaagt cgaaaatata gctaaaaaaa tatctgaaaa taaacaagaa
    20341 gaacctaaaa aatctaaacc taaggatgta tttattttag atgttgaaga taaacttaga
    20401 aatatatttg gaacaaaggt gaatatttct aaaggaaaga agaaaggtaa aatagagata
    20461 gaatactata atgatgatga tttaaatagt attgtatcaa tgcttcttga atagaacaaa
    20521 aatataaaac agagaggaag attgttatga tttatttaga taatgctgca actacatatc
    20581 caaaaccaga aagagtgtat aatgctgttt tagattgtat gaaaaattat tgtgcaaatc
    20641 caggtagagc aggccataaa cttgctatga gagctgctag ggagatatat gatacaagag
    20701 agaatatagc taagttgttc aatgtaagta atcctatgaa tatagtattt acatctaatg
    20761 caacagattc tttaaattta gcgataaaag gtgtattaca agaaggagac catgtaataa
    20821 caacgtctat ggagcataat tcagtaataa gacctataaa agcattggaa aaaaggggta
    20881 ttgaaaatac tgtagtaaaa tgcgattatg agggattttt agattatgaa gatttagaaa
    20941 aatcaataaa gagtaatact aaactgattg taacaacaca cgcatctaat gtatgtggaa
    21001 ctttaataga tataaaaaaa gttggagaaa tagctaaaaa acataatata ctatttttag
    21061 tagatgcatc acaaacagca ggtgtttatg atatagacgt aaatgaatgt aatatagata
    21121 tgttggcaat gccaggtcat aaatgcttat ttgggcctca aggaacaggt atattatatg
    21181 taagagaagg gttgaactta aatatattaa aagaaggtgg aacaggtagt aaatcagaag
    21241 aaatagttca accagagtta ttcccagata agtatgaatc aggaactcat aatactccag
    21301 gtatagcagg tcttaaccaa ggtatactat ttatatttga aagaggaatc aacaatataa
    21361 gacaacatga agaagaatta tgtcaatata tgatagacaa actagaggaa gtatcagata
    21421 taaagatata tggaccaaag gatagtaaaa aaagagcatc agtaatcgct ctaaatatag
    21481 gagatatgga ttctggagag gttactttct tattagatag tgattataat atagcaacta
    21541 gatcaggaat acattgttca ccactagcac ataccacttt aggtacttta aaacaaggtg
    21601 cagtaaggtt cagtatagga tatttcaaca caaaagatga gatagataaa gcagtggaag
    21661 ctttgaaaaa aatttcaaaa aataaatagc atgagattta ataatataga aataaaaaaa
    21721 ggggatattt caaaataaaa atttgaatca tctccttttt tgtacaaaaa agaaaagggg
    21781 ctcctaagaa cctctaatct atttgataat tgtttcaaga aattacttta agtaatctcc
    21841 ttcttcaaga actatatgac cactttcgta attagaacca tcaacagtaa tttttatttg
    21901 ttcaacattc atatttttta tgtaagtacg agcaactgaa tcaagcatta aagtttctgc
    21961 atcactacct aagtttgact ttgtaaagtc agagctaaca tctaatatac cagtttttac
    22021 accatcaatg tcttttgtag taaatgaatt taattttgca gattctggaa caactttaag
    22081 tttttgaagt tcttcaaata aagtgttctc atcaatttta tttaaatcaa ctttattttc
    22141 aattaaatct gtcttatcta catcaaaaga gtaaatagta gctgattgag ttttagtttc
    22201 ttcttttgga gtaggtttag ctttgttatt aggaacctct ttttcagtat tgttttcttc
    22261 ttttggttgt tcaacatttg tattgctctt agtttctttt ttagatgtat cacttccatt
    22321 ttgagcatta gtacaaccaa ctgcgaatat agatatagat aatatactta acactaacat
    22381 tattttctta tttttcataa aaatttctcc tctcttaaaa actattctta tatataatta
    22441 tagtcgttat gagcaaatat atgaatacaa aatagtaaca aatttaaaaa atgatttatt
    22501 taaattaata aaactcaaaa taaactaaat tttagtcatt tttatgggaa atcatttaat
    22561 gtacagtgaa aatattgtaa aataaaagta gtgtttttaa aataaatata agttataatt
    22621 atattataaa aaaatgtctt caaaaggtgg gaatagtatg gaaattttga gcttaggaga
    22681 aaagattaaa aaattaagaa aagaaaaaaa catgacatta aaagaattag ctggagatag
    22741 gataacagca gcacaaataa gccatataga gagggataaa tctcatacta gttatgaact
    22801 gttggaatat ctaggcgata agctagatgt aagtgtagaa tacttattag agaccaagga
    22861 aatgcaatca aaaaagataa ctgataattt aatactacag agcgaaattt atataaaaga
    22921 aaatgaatta aacaaggctg aggatcaaat aaaagaagtt ttagatatat gtaagagata
    22981 cgatttaatg gagaactatg gcagatgtaa ttttttaatg gctaatatta atttgaaaaa
    23041 atctaattat aatgatgcta tagaaaactt tgaaaaggca ttgtacttct ttataaaaaa
    23101 tagtgatagt gaaaatatac ttaagtgtta tttaaacata gggaaaatat atatgcagga
    23161 agacttttat aaaggtgcta taagccactt taattttgca gaggaaattt tgtcagagaa
    23221 taaatttgag gatgttaaca tacacaagga actatatagt aagatggcat attgttacat
    23281 aaaactagat aatccaaata tgtcattgaa gtacatagaa aagataaatg aaatagataa
    23341 tagaaatgat agaaaagaag atgtcgatat tttggtgtta aaagcaaata atatgcttga
    23401 gattggaaaa tatgaagaat caagagatta ctttaaaaaa gcactaaaaa tacttgagag
    23461 tgaggataat aaaactggat tagcaaatgt gtatttaaca atttgtgatg tttacagaaa
    23521 aataggtgaa acagatagag tacttgaata ttcacaaaag gtatatgaca taaagaaaaa
    23581 tgatgaagat gaatacatga tgtcaggatt atttaagata atagaagcat atatagataa
    23641 agaagattat gatttagcta gaaaatattg taagatagcc ttagcttcat caataaaaaa
    23701 taaaaataaa ttcaatgaat ataaagcatt gagattctat gctaatatgt ataaaaatca
    23761 gaatgaagta actcttgcaa ttgaatatct gattaaatgc ataaaaatag tatcagattt
    23821 aggcaacaat agaatattgg ccaatttata tatagactta ggtcaattat attcaaatat
    23881 atcaaaagaa aaagagttag aatattatca aaaaggtgta tttatgtaca aaaatttaga
    23941 aataatgtag ggtaaataaa cctataaggg tatagaaaca atctataaca agcaagataa
    24001 tatctatgat aaaatataaa ttgtatgaat atttttaagg agatgaatca gacaatgagt
    24061 ataaaaatag atgcgagagg gcttgcttgt cctaaaccag ttataaatac aaaaagagaa
    24121 ttggataacc tagaagaagg tgttgtagtt acaatagttg ataatgaaac agctaaggag
    24181 aatatattaa aacttgctaa atctttaagt tgtgaagcaa atattgttga cgaaaaagag
    24241 aattttatat ctatagaaat tagaaaaggt aaaaatatac tagataaaca aaatgttgta
    24301 gataataaag aaagtgaatt agacaataca tgtatattta tatcttcaga taagatggga
    24361 cttggaaatg acgaattagg aaaagtgtta ataaaaggat ttatatatac attaacagaa
    24421 tctaaacctt atcctaagta tgtactttta gttaatggag gagtaaaatt aagtgctgaa
    24481 aatgaagaaa ccatagaaaa tctaaaaata cttgagaata tgggtgtaga agttttatct
    24541 tgtggtactt gtttagatta ttataattta aaagacaaat tacaagtagg tacagttaca
    24601 aatatgtata ctatagttga gacattaaag aatgcatcaa ataccatttc tatataatgt
    24661 atgtttgttt aataatataa aactaataat aaagattaac tcaaatagat agtcagaaag
    24721 gattaagtaa tgaatcaaat gtatatagtg tcatttaatt cgacacatca tgcgataaga
    24781 tcagaaaagt tatttggtga aaatagtcta aaagtaatgg ctcttccaac accaagagaa
    24841 ataacagcaa gttgtggaat atcaattaaa ttttcttttg aagatatgga aaagataaaa
    24901 actattttgg ttgaaaataa tgtagatata aaaggaattt attgtataag taaattagaa
    24961 aatggttcaa aagaagtaga gaagttagat taggaggcta attatttatg ccaatggatt
    25021 taaaaatagg tgatattgtt gaattaaaaa agcaacatgc atgtgggtgt aaagagtttg
    25081 aaatcataag aacgggaatg gatataaaga taaaatgtac taaatgttca aggctaataa
    25141 tgcttgatag agagacctta gaaaagagag taaaaaagct aataaaaaag tgataaaaat
    25201 ttaacactta aaaaatgtca aataccgtat attaatatcc aaaattttgg tataataaga
    25261 attgaagaaa acattttcat gggggtgctt taattatggc agttaaatat gcaaaaagaa
    25321 tgcaaggatt acaaggatca gaaatacgtg agcttttaaa acttactcaa caaccacaaa
    25381 taatatcttt tgctggtgga atgccggctc cagagttatt tccagttgag gaaatgaaaa
    25441 aagtatcagt tgcagtgctt gaagaaaatg gaagatcagc tatgcaatat actacaacag
    25501 aaggatatga accattaaga gaaaaaatag cagctagaat gaatgataaa aacaaaacta
    25561 atgtaaacaa agatgatata ttagttacaa gtggttctca acaaggtcta gattttgcag
    25621 gaaaagtatt tattgatgag ggtgatgtaa ttttatgtga aagtccatct tacataggag
    25681 ctataaacgc atttaaatct tatcaaccta aattcataga tgttccaaca gattctgatg
    25741 gaatgataat ggaagaatta gaaaagatac tagaaacaac tgatagaata aaaatgattt
    25801 acgtaatacc tgacttccaa aatccaacag gaagaacttg gccacttgaa agacgtaaaa
    25861 aattcatgga aatagtaaat aaatttgaaa taccagtaat agaagataat ccatatggag
    25921 atttaagatt tgaaggagag actttaccat ctttaaaatc aatggataca aaaggattag
    25981 taatattctt aggaactttc tctaaaatat tctgcccagg atacagatta ggatggactt
    26041 gtgcttctcc agaaatttta tctaaattta actttgcaaa acaaggtgca gacttacaag
    26101 catcaactat atctcaaatg gaagtaagca aatttatgga tatgtatgac ctagatgcac
    26161 atgttgacaa aataaaagca gtttatgtta aacgtagaga tgtaatgctt aagactatgg
    26221 aagaagagtt cccagaagga ttagtattta ctcatccaga aggtggatta tttacatggg
    26281 tagagcttcc aagtaattta aatgctaaag aattaatgcc aaaatgttta gagaagaatg
    26341 ttgcttatgt tgctggagga ggattcttcc ctaatggtgg tagagaaaat actttcagac
    26401 ttaactattc aaatatgcca gaagaaaaaa taatagaagg tataaaaaat atagcagctg
    26461 tattaaaaga agctatgggt gtagaagctt aattaaaaat aataacttat atataataca
    26521 agggatggtt gttgccatcc cttgtttgtt tgaaaagtat ataattcacc ttatactaaa
    26581 ctttgtccaa aatttaaatt gacagaaata agaataaatg ataaaatgtg taggtatgag
    26641 attttagtct catttctctg ctctaagagt gtagagccgt taagtccaaa gggaggtgaa
    26701 ttatagtgag aaattatgaa ttagtttatg tggtaaagcc aaattctgat gaagaagtaa
    26761 gagaggctat actaaacaaa gttaaggaag ttgttgcaac tgacggagaa atagttaaag
    26821 ttgatacttg gggaactaaa aaattagctt atccaatagc taagtttaca gaaggtttct
    26881 atgtattagt taacttcaaa tcagcagtag acgttcctaa agagatagac agaaacttaa
    26941 agataaatga aaacgtaata agacacatga tagttgttgc ttaataactg gttttattat
    27001 aaaaaatagg tggtgttttt atgaatcaag ttgtattagt gggaaggttg actagagacc
    27061 ctgaattaag atacatccca ggtacaggaa caccagtggc atcctttaca atagccattg
    27121 atagagacta tgtaaaaaaa gatggatcta aagaaactga ttttatacct gttgaagtaa
    27181 tggggaaatc tgctgaattt tgtgctaact acataactaa aggtagatta gtagcacttc
    27241 aaggatctat aagagtagac aattatcaga cacaatcagg tgaaaagaga acttttacaa
    27301 aagtaagtac tagatcagta caagctttgg atagtaaaaa taaatcggaa aattcatata
    27361 gagagagtgc tccagctttt gagccaagct ttgaacctca aggtttagac ccacaaggtt
    27421 ttcaagctat agatgatgac gatataccat tttaaaaagg agggaaaaaa gtcatgatga
    27481 ataagaaaag acgtaagaaa aaaagagttt gtcaattttg tgctgacaaa aacgctaaaa
    27541 tagattacaa aagtactcaa agattacaaa agtatataac tgaaagagga aagatattac
    27601 caagaagaat atctggaact tgtgctaagc atcaaagaga attaacagtt gctataaaaa
    27661 gagcgagaaa tatagcactt ttaccatata cattagacta aaaaaatgag ccttgtgctc
    27721 attttttatt atatgtattt atatatctaa atgaggcgag ctataaagct cgtttttttg
    27781 tttatatact taaatatatt attatttatg atataatatt atatattaag gagggttgta
    27841 aacgtgaaaa tagataaagg ttctgagata acgagaaaca taaagataat agaatggatg
    27901 aaaacagaaa tattaatgag tgtaagtgat ttgtttaatt tattgtttaa aggggtaaaa
    27961 ccattagatg aagcaattca agatacttta gcaaatataa taatgataac atatctctta
    28021 gcaaagagat taggaattag ttttagagat gtggattata aagtcaagga aaaaataaaa
    28081 attggtatag atgaggacca tagcgttgaa agatggtatg gagatttatc taatttaaaa
    28141 aaacatatgg ataataggga gtgatattaa attgaataat aaaattagac tatctaaggc
    28201 aatgtctata atagttttag ctataataat cgctttagta atagcctata ttccaatgct
    28261 aggaatattt agtatgttag cagctgttcc atatgtaatt ataggtgcta ttactgatag
    28321 aaagtattct cttatatcta ttttggttac attttgtgta ctgattctat ttgcagacat
    28381 ttcttatgca ttaaatatat gtataatgta ttctttacca ggtatagtaa taggaaagat
    28441 gctaaagaga agctttgaac aagaggacag taataaattc gaaccgatat atggtggaac
    28501 cataatattt gtactttcaa tgcttgtata tttttttgta ttaaagacat ttttaaatgt
    28561 gaatctatta gatgaagttt ctaagatgat atcagaaatt gtaaatattc aaaaaaatag
    28621 tttttctgca actgaactta aagtgtttga taaaatgaag ccagatgaga ttgtaagtta
    28681 ttttacaaat atgctaccaa tgatgttatt tttacaaggg ttgttatcag cgtttgtcac
    28741 atattatttg agtgtatttt ttataaaaag gattacaaag atgaatataa gatttcctaa
    28801 atttgctgat ttttatttac caggaaatgc tatactcaca actttcttac tttatttatt
    28861 agtcttattt attcaaatta ttggttcaaa attatataca gaattaataa tgactaattt
    28921 acagctagtt tttaatttaa tgtttgtaat tcaaggtata gcagtttgca tttatttctt
    28981 aaagaaatgg ataagacagg gaccaaataa aattatgttt ttttcaggga taattttatg
    29041 tttatttgga tttatgggaa tatcttttgt tggtatggta gatagtataa ttgactttag
    29101 aaaggtaaga agctataaat ccacttagga ggatagaaat gagtaacaaa caaaccttta
    29161 aattaaatat gccagaaata aatttatata taattgttat tggtatttct agtataattt
    29221 tgctttatta caacttgtat gtagggtgtt tatttttctg tatttttgta tatatggttt
    29281 ttcacaattg gagaactact aatattagac gtcatgaatg gacagagtat attcaaaatt
    29341 tatctttaga tatagatgaa acaactaaaa aagccataat aaatcttcca ataccattat
    29401 gtattttaga atttgatgga aatatatcct ggtataatgg aaagttttat gatatgattg
    29461 gtcaaaaaga tttacttgat aaaaatatag aggatatagt taaaaatctt aatttaagaa
    29521 aagtattaaa tgaaaataaa gagatgtaca cagaaataaa ctataaagaa aaagaatata
    29581 caataattta taatgttata aaaaatgatc aagaaaaaaa tcctaaatat ttgatgatat
    29641 tatactggat agacaagact gaatatttaa aagtaaaaca aaattatgat gatgaaaaaa
    29701 atgcaatgat gctaatacaa gtagatggat atgatgaagt attaaaaagt gcagcagaag
    29761 ataagagagc tttaattaat gttgaagtag agaaaatact ttctgcttta gaattaaatt
    29821 caaatggtgc attgagaaga acttcaaaag acaaattctt cttggtaatg cataaaaaag
    29881 agctaaaaaa attggaagct gagaagtttt ctattttaga tacaataaga catattgatt
    29941 atggaaataa tcttcctgtt actataagta taggaattgg tatagatgga gatacattaa
    30001 atgagaacct aaaacttgct acgggtgctc tcgatttagc acttggtaga ggtggtgatc
    30061 aggctgttgt aaaaacaaaa gataaatttg tgttttatgg aggaaaatct aaagctgtag
    30121 aaaagaaaac caaagtaaaa tccagattaa taggtcatgc tttaagagag gttatacaac
    30181 aaagtgacca agtttatata atggggcata aatatcctga tatggatgct atgggagcag
    30241 cagttggtgt ttatgatata tgtaaatcct gtaacaaaac tgcaaacata gttttacaat
    30301 ctgtaaatga atctatagaa atatttataa ataaaataaa tgaaaataat tactataaaa
    30361 agctgtttat tggaaaagag gaagctattg acaactgtac taaaaataca ttagttgtag
    30421 tagttgatac tcatagacca aattatactg aatgtgaaga actgttaaag ctatcagaga
    30481 aggtagtagt tatagaccat catagaagag gtgtggagtt tataaatgat gcagtgcttt
    30541 tattccatga gatatatgta tcctcaacgt gtgaaatggt tacagagtta gttcagtata
    30601 tggatgaaga tgtaacaata aataaactta cagctgaagg gcttttagca ggtataagtc
    30661 tagatactaa aaactttgca ttcaaaacag gtgttaggac ttttgaagct gcttcttacc
    30721 ttagaaaagt tggagcagat actatagagg taaagaaatt cttcaattct gatgtaaaag
    30781 atttcataat aaaagctgaa ataattcaaa gtactaaaat aattaataat agaatatgtt
    30841 tagcatattc aagtactgag atagatagta taaatgttat aatagctcaa actgctgatg
    30901 agttattaaa tatcaaagaa gtagaagctt cctttgtatt aggtgaaaaa gatgatacta
    30961 tatttataag tgcgaggtct ttagggcaaa taaatgtaca tgtacttatg gaaaaattag
    31021 gaggcggggg acacatagat atagctggag cacagttaaa aaatgtttct ctaaaagaag
    31081 cttataagat ggtaaataaa ataatagagg agtatttgga ggaggaagaa tagatgaaag
    31141 ttatattatt aaaagacgta aaaggaactg gtaaaaaggg agaaatgaaa gaagtaagtg
    31201 atggatatgc aagaaatttc ttatttccta aaaagatggc tgtgcaagca gatagtgtag
    31261 caataaaaga attaaatgaa aagaataaat ctaaagaaat aaaagcacaa aaagagtatg
    31321 aagaagcagt tttattaggt aagcaaatgg aagaaattaa tatagaaata tattcaaaag
    31381 caggagaagg tggaagatta tttggttcaa taacttctaa agaaattgct gagcaattaa
    31441 agaaacaaaa agacatagac gtagataaaa gaaaaatatt attagatgaa ccaataagat
    31501 ctttaggttc aactttcgta gaaataaaaa tacatcaaaa agtaactact aaaataagag
    31561 tggatgtaaa agaaaaacag taataactag aggtgattaa gatggaagat atgacgagaa
    31621 ttcctcctca tagtgtagaa tcagaacaat caatattggg ttctatactt ctagataaag
    31681 atgctataat aacggttaca gaaactataa agcctgatga tttctataaa gaggctcaca
    31741 aaataatata tgaatgtatg ataactttaa gtaataaagg tgagcctata gacttgataa
    31801 cattaacaga agaactaaga aaacaagggc atcttaatga tattggagga atcagttata
    31861 ttacaagtct ttctactata gtaccgacga cttcaaatgt aaaatactat gctgatatag
    31921 taaaagaaaa gtctgtactt agaaagctta taaaagcttc taatgaaata ataaatctag
    31981 gatatagtgg agcaactaag attgaagatg ttttagaaca agctgaaaag agtatatttg
    32041 atatatcaca ggagaaaact agtgatgatt ttaaatctat aaacttagtt ttgatggatg
    32101 cctatgatat gatagaaaaa ttatatacta acaagagtga tgtcactggt ataactactg
    32161 gttttaaaga tttaaacaaa aaaataaatg gtcttcaaag gactgattta atattaattg
    32221 cagctagacc agcaatgggt aaaactgcct tttctttaaa cttggttcaa aatgcagcct
    32281 taaaaggtga tgcctctgta gctgtattta gcttagagat gtctaaggaa caattagtac
    32341 aacgtatgtt atcttcacaa tctagtgtag aattaaaaaa aataaagact ggaacactta
    32401 atgataatga ttggcctaga attatagatg ctatggcagt attatcagat gccaaaatac
    32461 acatagatga tactcctggt ataaaaatat cagaactaag gtcaaagtgt agaaagttaa
    32521 aaatagaaaa aggactagat ttagtgctta ttgattatct tcaacttatg gaaggtgaag
    32581 gtaataatga aagcagacaa caagagatat ctaagatatc aaggtcacta aaaatattag
    32641 caaaagaatt aaactgtcct gtagttgcac tatctcagtt atcacgtgca ccagaacaaa
    32701 gagcagacca tagacctatg ttatctgatt taagagagtc tggagcaata gaacaggatg
    32761 cagatatagt tatgttctta tacagggacg aatattatca tgctgattct gagagtaaaa
    32821 atataggaga agtaataata gcaaaaaata gacacggtga aactggttct gtggaacttg
    32881 tttggcttgg tgaagttcaa agatttgggg ataagttgag agacctataa tctaacgaat
    32941 atccacagaa cgtcctttct ttctacagga aaaatgtgga tacatgtcac taaatatgtc
    33001 caaacattga aatttagcta aaaaactatc cacactatcc acagatagcc tgttgataag
    33061 tgtgaataag tagataaagc aagcttttgg cttgctttat ctgtgtataa atattaatta
    33121 ttaggaggaa taatatggaa aaaataagct taaaatatat atatcccaat ataattaaag
    33181 tacttgatga aataaactta tttagagtaa ttgacaataa tttaagagag tctattgttg
    33241 tttatgctaa taatgtggat aatcagtatc atataaacat gactaataca aactttggaa
    33301 acataattaa catatgtaaa ttagaaaaac ttttagatgt ggataaattt atggaaaaag
    33361 ttataaaata tgaaaaagaa ataatagaga aagaagagtt ttcaaaaata gaagaatata
    33421 tgttaaatat tggagaatac taaaaaagtt gaaaaaaata cgaatgtttg atagaataag
    33481 taagtaaagt gttcgagagt atataaaata tagaggtgaa ttacatgaaa acagttgcaa
    33541 ttgttggttc tcaatgggga gatgaaggta aaggtaaagt tatagattat cttgctactc
    33601 aagcagatgt agtagtaaga gggcaaggtg gaaataatgc aggacataca ttagttgtag
    33661 aaggtaaaaa atacgcatta catctaattc catctggagt tttaaatcca aatacagtga
    33721 atataatcgg aaatggaata gtgtttgacc ctaaaggatt tttagaagaa ttggaaatgt
    33781 ttaaaacgga taatataagt acagaaaata taaaaataag tgatagagca catgtcatat
    33841 tcccatatca taaagaacta gatgcattgt cagaagaagc cagaggcgat ttaaagatag
    33901 gtacaacaaa aaaaggtatt ggaccttgtt atatggataa aacagagaga tctggtatta
    33961 gaatatgtga cttaatggat aaagataaat ttgctataaa attaaaagct caaatagatg
    34021 ctaaaaataa aatagttaaa aatatatatg gaaaagaaga attatttgat tttgaaacta
    34081 tatataacga atatctagga tatgcagaac aaataagaaa atatgtggca gatacgtctg
    34141 ttattgtata tgatgcagta agagcaggta aaaaagtatt gtttgaagga gcacaaggga
    34201 ctttattaga tttagattta ggaacttatc catttgttac atcttctcat ccaacatctg
    34261 gtggatttgc aattggtgca ggtataggac caaatatgat aaaagacgtt gttggtatag
    34321 taaaagcata tactacaaga gttggagaag gaccgtttgt tacagagcaa ataaatgaaa
    34381 ctggagataa gataagagaa caaggacatg aatttggggt aactactggt agaccaagaa
    34441 gatgtgggtg gtttgatgca gttatagtta agtatgcagc tagagttaat ggattgacta
    34501 gtatttcatt tatgttactt gatgtattaa ctggatttga taagataaaa gtttgtacgt
    34561 cttataaaat gggcgataag ataataacgg atttcccagc ttcattagat gatttagcaa
    34621 aatgtgaacc tgtatatgaa gaattagatg gatggaatga agatataact caaatagata
    34681 actttgatga ccttccagaa aatgcaaaga aatatgtagc aaaaatagaa gaactagttg
    34741 gagtaagtgt agatatggtt tctgttggtc caaatagagc tcaaactata ataagaagaa
    34801 atatatttgc ttaatatttt atcaattaaa taatttaaaa tagttgaaga agagtatctc
    34861 aagataatgt ttatagttat tagagatgct cttttatatt tgacttacat tgatttttta
    34921 ttaagttaaa gctacttata catattctag ctgaaattaa agttgaattt atgtagatac
    34981 atataggttt tttaaaaaga attttgttga aaataaatgg ctaaagatga ttttaattat
    35041 attctaaaaa aagtaaaaaa aactattgac taaaaattgt atagacgcta taatataact
    35101 tgtgagtgaa agaaacgacg aaaaaagtcg aaaaaatgtg gtctattagc tcagtcggta
    35161 gagcacctga cttttaatca gggtgtcccg cgttcgagtc gcggatagat caccaaaata
    35221 tggctcgttg gtcaagaggt taagacaccg ccctttcacg gcggtaacag gggttcaatt
    35281 cccctacgag tcaccagtat gggactatag ctcagctggg agagcacctg ccttacaagc
    35341 agggggtcac aggttcgagc cctgttagtc ccaccaaatt ttgaattaga gataaatact
    35401 agattatatg ctggtgtggc tcaacggtag agcagctgac ttgtaatcag caggttgtag
    35461 gttcgattcc tatcaccagc tccacaaagt acataaattc aaattaatat aattttctat
    35521 gttacaggat acttttaagt atcttttttt aatttcattt ttattgtatt aaaaatgaaa
    35581 ttaaacatac aacaaaattt tgaattactt gcaatgtgat agttttaaat ataaaattta
    35641 gaat
//
LOCUS       DACTGW010000011        34870 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.011, whole genome shotgun sequence.
ACCESSION   DACTGW010000011 DACTGW010000000
VERSION     DACTGW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.011"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            120..596
                     /gene="purE"
                     /locus_tag="I9573_000462"
     CDS             120..596
                     /gene="purE"
                     /locus_tag="I9573_000462"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAT4795096.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            602..1300
                     /locus_tag="I9573_000463"
     CDS             602..1300
                     /locus_tag="I9573_000463"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAT4795097.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            1312..2679
                     /gene="purF"
                     /locus_tag="I9573_000464"
     CDS             1312..2679
                     /gene="purF"
                     /locus_tag="I9573_000464"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAT4795098.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            2673..3749
                     /locus_tag="I9573_000465"
     CDS             2673..3749
                     /locus_tag="I9573_000465"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAT4795099.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            3743..4336
                     /locus_tag="I9573_000466"
     CDS             3743..4336
                     /locus_tag="I9573_000466"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAT4795100.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            4329..5861
                     /gene="purH"
                     /locus_tag="I9573_000467"
     CDS             4329..5861
                     /gene="purH"
                     /locus_tag="I9573_000467"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAT4795101.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEVLKQKKNLRILKLAKIEDSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            5872..7122
                     /gene="purD"
                     /locus_tag="I9573_000468"
     CDS             5872..7122
                     /gene="purD"
                     /locus_tag="I9573_000468"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAT4795102.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKVYCAPGNAGITNVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMETILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMLDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            7147..10953
                     /locus_tag="I9573_000469"
     CDS             7147..10953
                     /locus_tag="I9573_000469"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAT4795103.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAVPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKTFTDDKLFE
                     ASYGNIVLELANDDLCMLEGYNYVVLGSTTKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERVGA
                     NANIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVFKNIIGEKDQKIFES
                     GVKYFR"
     gene            11159..12031
                     /gene="rfbD"
                     /locus_tag="I9573_000470"
     CDS             11159..12031
                     /gene="rfbD"
                     /locus_tag="I9573_000470"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAT4795104.1"
                     /translation="MKILITGSNGQLGKELVSQLEAINQSINQPKYVILATTRNDLDI
                     SNQTNIDNFILHNKPDVIVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LVHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFELKNIDIKVNPIKSNEFKCKAQRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            12140..13021
                     /gene="rfbA"
                     /locus_tag="I9573_000471"
     CDS             12140..13021
                     /gene="rfbA"
                     /locus_tag="I9573_000471"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAT4795105.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLKITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDMQYGR"
     gene            13058..13615
                     /gene="rfbC"
                     /locus_tag="I9573_000472"
     CDS             13058..13615
                     /gene="rfbC"
                     /locus_tag="I9573_000472"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAT4795106.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFCDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVRYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFNYKCTNYYYPEFEHGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICIRR"
     gene            13653..14636
                     /gene="rfbB"
                     /locus_tag="I9573_000473"
     CDS             13653..14636
                     /gene="rfbB"
                     /locus_tag="I9573_000473"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAT4795107.1"
                     /translation="MKKILITGGAGFIGSNFVHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDSSNYKFVKGDISNREQMYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSPINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLDKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            14675..15343
                     /locus_tag="I9573_000474"
     CDS             14675..15343
                     /locus_tag="I9573_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAT4795108.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNSSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSENLFDELGSVVNNTASRFIDVDTKDKSVKSRIENAIEQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            15352..15780
                     /locus_tag="I9573_000475"
     CDS             15352..15780
                     /locus_tag="I9573_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HAT4795109.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            15804..16118
                     /locus_tag="I9573_000476"
     CDS             15804..16118
                     /locus_tag="I9573_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HAT4795110.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            16270..16545
                     /gene="flgM"
                     /locus_tag="I9573_000477"
     CDS             16270..16545
                     /gene="flgM"
                     /locus_tag="I9573_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HAT4795111.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGVKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            16550..16966
                     /locus_tag="I9573_000478"
     CDS             16550..16966
                     /locus_tag="I9573_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HAT4795112.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDRYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTATYNSYGNIRK"
     gene            16984..18294
                     /gene="flgK"
                     /locus_tag="I9573_000479"
     CDS             16984..18294
                     /gene="flgK"
                     /locus_tag="I9573_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HAT4795113.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETIINEPSDYGISAGIDDFFKGWESLAKDINSDSKKSLVVENASNLATLISESYK
                     KLNKLKGDIDDNINSEIKQINDMLKSLEDLNKSIDVISGSGSTPNDLLDERDRILDDL
                     SFKLDLENSDVKNMLSDGKLELNELKNADGTWKTGVSGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKVNPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            18313..19245
                     /gene="flgL"
                     /locus_tag="I9573_000480"
     CDS             18313..19245
                     /gene="flgL"
                     /locus_tag="I9573_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HAT4795114.1"
                     /translation="MRVSTGMMSSNYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            19261..19653
                     /locus_tag="I9573_000481"
     CDS             19261..19653
                     /locus_tag="I9573_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HAT4795115.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            19647..19859
                     /gene="csrA"
                     /locus_tag="I9573_000482"
     CDS             19647..19859
                     /gene="csrA"
                     /locus_tag="I9573_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAT4795116.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            19881..20276
                     /gene="fliS"
                     /locus_tag="I9573_000483"
     CDS             19881..20276
                     /gene="fliS"
                     /locus_tag="I9573_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAT4795117.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            20276..20632
                     /gene="fliS"
                     /locus_tag="I9573_000484"
     CDS             20276..20632
                     /gene="fliS"
                     /locus_tag="I9573_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAT4795118.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELIRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            20653..22176
                     /gene="fliD"
                     /locus_tag="I9573_000485"
     CDS             20653..22176
                     /gene="fliD"
                     /locus_tag="I9573_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAT4795119.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLKVIGNDNLAQQIANDNGIT
                     FANDASGNKVASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            22179..22514
                     /locus_tag="I9573_000486"
     CDS             22179..22514
                     /locus_tag="I9573_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HAT4795120.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISSKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            22616..23488
                     /locus_tag="I9573_000487"
     CDS             22616..23488
                     /locus_tag="I9573_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HAT4795121.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTDAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            23581..25722
                     /locus_tag="I9573_000488"
     CDS             23581..25722
                     /locus_tag="I9573_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAT4795122.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRSSIESELIIVDTGSTDD
                     TINISRKYTENVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNIDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFEELKENDNSPYTNYQLGKNFIILGKYHEAL
                     YYLEKSYELYSKLESAPGYLITSLVKTYLYLGKHKKCEKLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYESVISEYNNIEDMEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YKNIEEVINNIKDDEKEHVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKVAFEDKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDI
                     KKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYRNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNDANEKLEYIHSMRKLLNEYPFYNKIIKILISELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKSLVDDYSKTFMDDVKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            25746..27623
                     /locus_tag="I9573_000489"
     CDS             25746..27623
                     /locus_tag="I9573_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAT4795123.1"
                     /translation="MNTPIVVNEKSKDIIKDWQYTYTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFMFAHKINTSNINLWKYRLKLVGFCMLIKKSVLDNVGVLDELFTPGNFEDDDLSFR
                     MLESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIADIEKVKLDYEKDFFDYIILGDVLEHLTDPWKIVNELKQYLKKDGCLISSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSVTATSMPLSEID
                     NRFIDTMCKESCKEMRQQYESYQYIVKATKSIDTKRYSSISMVNLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLVDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSKKVQSESDIQDILYFIEGKRY
                     E"
     gene            27616..29730
                     /locus_tag="I9573_000490"
     CDS             27616..29730
                     /locus_tag="I9573_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAT4795124.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDAFIINKNFIYDILEIFKKNTDVGMIGVAGVKDI
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVANINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLDIVSKIAHFS
                     VFNHTGLYSCEKCERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAIDDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKEKQSEIKHFVIDKHCIEIKNYIDKIYKEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            29739..30734
                     /locus_tag="I9573_000491"
     CDS             29739..30734
                     /locus_tag="I9573_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HAT4795125.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIIYKGEEILAVVPACETYENGERVFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLETYLQEQSFNKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKK
                     DILANFRYSKRRYFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            30826..32058
                     /locus_tag="I9573_000492"
     CDS             30826..32058
                     /locus_tag="I9573_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HAT4795126.1"
                     /translation="MKRLMILGSIKYFENIVMSAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEIFDENNENMIENF"
     gene            32078..33022
                     /locus_tag="I9573_000493"
     CDS             32078..33022
                     /locus_tag="I9573_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HAT4795127.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSDIFLYNMKDAKLKAILDGNYITA
                     FRTAAVAGLAIDTFAKKDFNTISIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFIKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYNVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLNCEEIELKEPKEKYWI"
     gene            33116..33523
                     /locus_tag="I9573_000494"
     CDS             33116..33523
                     /locus_tag="I9573_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HAT4795128.1"
                     /translation="MDKYNLIKFLSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKESIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            33610..34701
                     /locus_tag="I9573_000495"
     CDS             33610..34701
                     /locus_tag="I9573_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HAT4795129.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVRHFEEE
                     FASYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASKMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFVGMEDEEVSYIIKIINEY"
BASE COUNT        14309 a         3468 c         6451 g        10642 t
ORIGIN      
        1 atttcaaaaa agagttgata atatatgtat attaatataa aatttaaggt gtataattcg
       61 tataataata cgaatgattt gtagtgtaaa ataacttata aacggagagg ggaagtctaa
      121 tgaaagtagc agtagttatg ggttcaaaat cagattatcc aaaattagaa gaagggataa
      181 aactgcttga aaaatatgga atagaggttg tagcaagagc attatcagct catagaacac
      241 cagaacaact ttcaatattc ctaaaagaaa tagaagatga tactgatgta ataatagcag
      301 cagcaggaaa agcagcacac ttaccaggtg taatagcttc acaaacatta attcctgtaa
      361 taggattacc tattaaatca tcaactatgg atggactaga ctcactttta tcaatagttc
      421 aaatgccaaa aggaatccca gttgcaacag ttacaataga cttaggttta aacgcagctt
      481 tattggcatt acaaataatg actttaaaat acccaaaatt aaaagaggat ttgaaatcat
      541 atagagaaga gatggcacaa aaggtgttag aagacgacaa gaatttaagg gggtagcaga
      601 aatgttatta tatgaaggaa aagcaaaaca agtatattct acagataatg aaaatgaata
      661 tgttgtttat tataaagatg atgcaacagc atttaatgga gaaaaaaaag cagaaatatc
      721 ttcaaaaggt atactaaata ataagatatc aacaataatc tttgaaatgt taaaagaaaa
      781 taatataaat actcacttta taaaaagttt atcagataga gaaatgttag ttaaaaaagt
      841 agagatttta ccattagaag taatagtaag aaatattgct gctggttcta tctgtaaaag
      901 agttggtctt gaagaaggtg tagttttcga tgagccaatt tttgaaataa gttacaaaaa
      961 tgatgcttat ggtgacccaa tgctaaatga tgattatgca gtggcaatga agttagcaac
     1021 tagagaagag ttaaaattct taagagaaga aactttaaaa ataaacgagt tactaaaagc
     1081 atttttctta aaattaaacc taaagttagt tgactttaaa atagaatttg gtaaggattc
     1141 tgaaggaaat ataatattgg cagatgaagt atcaccagac acttgtagac tttgggatgt
     1201 aaatacaaat gaaaagttag ataaggacag atttagaaaa gaccttggag accttgtaga
     1261 aggttatacg gaagtacttt caagaatgaa taataaatag gaggcaaatg tatgtgtgga
     1321 gttttaggga tttactctaa taaagatgta actaaagaac tatattattc tttatattct
     1381 atgcaacaca gggggcaaga aagctgtggt ttagctcttt tagatgatgg agaaattaag
     1441 tacaaaaaag atatgggatt agttggagat gtttttaagg aaaatgaatt atctaagtta
     1501 aaaggaaata tagggattgg acatgttaga tattcaacag ctggaggaag tcatgtttct
     1561 aactgtcaac ctttagttgg aagttgtaga aaaagacaat tagccatagc tcataatgga
     1621 aatctagtta atgcaaatta tttaaaagat atgctagaag aagatggata tatgttccaa
     1681 actaattcag atacagaagt gattttatat atacttgcta gatattacaa aggggatata
     1741 gtagaaagtt taaaagtgac aatggattat ataaaaggtg catatgctct tgtaataatg
     1801 agtcaagaag aattggttgc agttagagac ccacatggat tcagacctct tgtacttggt
     1861 aaaaagggtg atgaatatat atttgcatca gaaaattgtg caatagatat acttggcgga
     1921 gaagttataa gagatgtaga gccaggagag ataatagtag tcaaagatgg agaactaaaa
     1981 tcatattttt attcagaaaa ttataaacca gttaagaaaa gttgtatatt tgaacacata
     2041 tactttgcaa gaaatgatgc aactatagac aatgtaaatg catatgaatt tagaattaaa
     2101 tgtggtgaga gattagctca gaatgaaact gtaaaggcag atatggtagt tccagttcca
     2161 gattcaggtt ggccaggagc tattggttat gcaaatgcta gtggattaaa aataagtgaa
     2221 ggcttagtta aaaatagata tgtaggaaga acatttataa aacctacaca agaagaaaga
     2281 gaaatagcag taaaaataaa attaaatcct ctttcaacaa taataaaagg aaaatctata
     2341 attttagtag acgactcaat tgtaagagga actacatcta aacaattagt aaaatcttta
     2401 agagaagcag gagcaaaaga aattcatctt agaataactt ctcctccagt agcttactct
     2461 tgttactatg gaatagatac tccaaatcgc tctaaattaa ttgcatcaag taacaacgta
     2521 gaagaaatga gagaatatat tggatgtgat agtttaaaat tcctagatat agaaggaatg
     2581 ttagatgcaa cagaacataa gtctactttc tgtaaagctt gttttgatgg agaataccca
     2641 gttaagaaaa ttgataagga ggaactactt tcatgttaac atataaagaa tcaggtgtag
     2701 atatagatga aggaaataga gcagtagacc ttataaaagg taaaataaaa ggaacttatg
     2761 atggaaatgt agttggagat ttaggtaact tcagtggatt atacagttta aaagattttg
     2821 taggtatgaa agaaccagtt ttgcttgcat ctacagatgg tgttgggact aagttaaaaa
     2881 tagctcagat gatggacaag catgatactg ttggaataga tttagttgca atgtgcgtga
     2941 atgatttaat ttgtcaagga gcaaaaccat tatttttcct tgactatata gctcttggga
     3001 aattagtgcc tgagcatatc gaaaaaatag ttggtggaat tgcagatggt tgtaaaatgt
     3061 ctggttgtgc actaattggt ggagaaactg ctgaaatgcc tggaatgtat ggagaagatg
     3121 actatgattt agcaggattt tctgtaggaa tagcagacaa agaaaagatt gtttctggaa
     3181 acaatgttaa atcaggagat gtattggttg gtatatcttc aagtggagta cacagcaatg
     3241 gattttcttt tataagaaag atatttttag aaacttacaa ttataaaatg gaacaatatg
     3301 ttgaagaact aggaatgaca gttggagaag cacttttaac accaactaaa atatatgtta
     3361 aattagcttt agatgtatta gctaaacatg acataaaagc tatagctcac ataactggtg
     3421 gagggcttat agaaaatata acaagagtta tacctaaagg gttaggacta gatatcaata
     3481 aaaaatcttg ggagaagcct cctatattta aaatgataga aggttttaat gctgttgatg
     3541 agagagaact tcataagagt tttaacatgg gtattgggct agttttaata gtagataaag
     3601 aaaatgctga tgatgttgta aactttataa ataatagaga aaatgacaat gctgcttatg
     3661 tagataaaaa atacagtgaa ttattagaag ataaagcgta tataattggt gaagtagtag
     3721 atagtcatga aggtgtagaa ttatgctaaa tataggagtt ttaatatctg gtggaggaac
     3781 aaatttacaa gcagttatag atggcactga gtctggagaa ataaaaggtc aggttaaagt
     3841 agttatctca agtaagcaag gtgcttatgg gctagagaga gctaaaaatc ataacataaa
     3901 agcaatttgt gaaactgatg aagacaagat aatagagatt cttaaggaaa ataaaataga
     3961 tttagtagtt ttggctggtt atttaaaaat aatcagccca aagctggtta atgagtttag
     4021 aaacaaaatg ataaacatac atccatcatt aattccttca ttttgtggag ctgggttcta
     4081 tggagaaaaa gttcatcaag gtgttataga ttacggagct aaagtaactg gtgctacagt
     4141 tcattttgtt gatgaaggtg ctgatactgg accaatcata atgcaagatg tagttaaggt
     4201 aaatcaagat gatgatgcaa agacacttgc aaaaagagtg ttagaagttg aacataggat
     4261 tttaaaagaa agtataagtc ttttttgtga aaataaatta aaacttcaag gtaggagagt
     4321 gtttataaat gagtaagaga gctttaataa gtgtaactga caagactggt gtagtggaat
     4381 ttgctaagga gttaaataag ttagattatg aaattatatc tacaggaaat acatttaaaa
     4441 cattaaaaga aaatggtgtt aatgtaatgc aagttgaaga tgttacaaat tttccagaaa
     4501 tattagatgg gagagtaaaa acattaaatc catatataca tggtggtata ctttataaaa
     4561 gagataaaga atctcatgta gaaactgtga atgagcataa aatacattca atagatttag
     4621 tagctgtaaa tctatatgat tttgaaggaa ctttaaaagc tggaaaaagt catgatgaaa
     4681 taattgaaaa tatagatatt ggtggaccat ctatgatacg ttctgctgcc aaaaactata
     4741 aagatgtaat agtagtagta gatataaaag attatgattc tataatagaa aaattaaaaa
     4801 cagatacaat gacattagaa gacagaaaaa aattatcata taaagctttc tcaactacag
     4861 gaagatatga tgcgttaata tctagttatt ttgctggaga agttggagat acatatcctg
     4921 acatattaaa tttaactttc caaaaagaac aaactttaag atatggagaa aatccacatc
     4981 aaaatggttt cttatattca caatcaaatg caaaaaatcc aatcttaaat tatgagcaat
     5041 taggcggtaa agaactttct tttaacaatt taaatgattt acatggatgt ttagaagtaa
     5101 tgagagaatt taaagatagt gaagaagtag tttcagtagc tataaaacat gctaattcat
     5161 gtggggtagg tctaggaaaa gatgcttttg aagcatatac aaaatgttat gaagcggata
     5221 aggtttcaat atttggagga atagtaggaa taacttctac tatagataaa gctacagcag
     5281 agaaattaaa tgaaatattc ttagagatag tagtagcata tgattttgag ccagaagctt
     5341 tagaggtact taaacaaaag aaaaatttaa gaatattaaa attagctaag atagaagata
     5401 gtttacaacc atatgaaatg aaatatttag atggaaaatt attaatacaa gatagaaata
     5461 atatattagc agaaaaaagt gaaaatgtaa caaaagcaaa accaacagat gcacaactta
     5521 aggacatgga atttggtatg agagtagtaa aaaatatgaa atcaaatgca attgcgatag
     5581 taaaaaatgg tcaaacttta gctttaggtt gtggacaaac atcaagaatt tgggcattaa
     5641 aaaatgctct tgaaaataac aaagataaag attttacagg agctgtttta gcgtcagatg
     5701 catttttccc atttgatgat tgtgtaacat tagctcatga atatggaata tcagcagtag
     5761 ttcaaccagg tggctctata aaggataaag attcaataga agcttgtgat aagtatgata
     5821 tggttatggt atttacagga ataagacact tcaaacacta aggaggatat tatgaagata
     5881 ttggttgtag gcggtggcgg aagagagcat gctatatgct ggaaactaag caaagaaaaa
     5941 aatgtagaaa aagtttattg cgcgccaggt aatgcaggaa ttacaaatgt agcagaatgt
     6001 gtaaatatag gtgatactaa tatagaagaa cttttaaaat ttgccaaaga aaatgaaata
     6061 ggtctgacaa tagtaggacc agaagtacca ctagttatgg gaatagtaga tgagtttgaa
     6121 aaagaaggat taagagtatt tggacctaat aaaaaatgtg ctcaacttga aggaagtaaa
     6181 gctttttcaa aagaatttat gataaaacat aacataccta ctgcaaaata taaagaatat
     6241 acaaacttag aagaagctat atcagaaata gatagttttg gatatccagt agtaataaaa
     6301 gcggacggat tagctgctgg aaaaggtgtt gtaatacctg aaaatagaga agatgctata
     6361 gcaactttaa aagaaatgat gtcagataag aagtttggag ctgctggaga taaaatagta
     6421 attgaagagt ttttaaaagg aatagaaact tcaattctag catttgtaga caatgataca
     6481 atagttccta tggcaagtgc aaaagaccat aaaaaagtaa acaactatga gcaaggtcca
     6541 aacactgggg gaatgggaac tttttcacct agtgaaatat atacagaaga attagcaaat
     6601 aaagtaaaag agacagtatt agaaaaaact ttagaaggct tcaaaaaaga tggacttaat
     6661 tttaaaggta ttttatttgt tggtcttatg ataactgaag atggagaaaa agtacttgaa
     6721 tataatgtaa gatttggaga ccctgagaca caatctgttt tatttagatt agaaacagat
     6781 ttacatgaga taatggaaac tatactagat aataagttaa aagatataga aataaactat
     6841 agtgatgatg aagctgtatg tgttatgctt acatcaggag ggtatccaga cagttatgaa
     6901 aaagggaaaa ttataactgg tcttgaaaac ttggatgatg atatagtagt attccatagt
     6961 ggcactaaga tgcttgatgg taatttagtt accaatggag gaagagtaat tggtataact
     7021 gcaaagtcaa ctacagttaa ggatgcagca gaaaaagttt atgaaaacat aaagaagata
     7081 aactttgaag gaatgcacta tagaacagac ataggaagat aatttttcta aaatggagga
     7141 taaggtatgt tgaatactga aaataaagac tcaatggtta gaagagtatt agttgaaaaa
     7201 agagaaggtt ttgaccttga ggctaaagct ttaaaaaaag acttagtaga aagtttacac
     7261 atagataata tagaaaattt aagaatatta aatagatatg atgtagaagg aatatcagaa
     7321 gaagtttatg aaaatgcagc aaagacaata ttctctgaac caaatttaga tgtagtatat
     7381 tatgaagaaa taccaaaatt aaatgatgaa agagtatttg caatagaatt tcttcctggt
     7441 caatacgacc aaagaggtga ttgggcagct caatgtgtac aaattgtaaa tcaagggata
     7501 agacctgcta ttaatacagc aaaagtttat atattatcag gcaaaataac tgatgaagag
     7561 ttttctaaaa taaaagatta ctgtataaat ccagtagata gtagagaagc atctttagaa
     7621 aaaccagaaa cattaaaaat ggaaactgaa ataccaacaa cagttgaagt attagatgga
     7681 tttatagatt tagatgaaaa tggattaaga acatttgtaa gtgaaaaagg attagcaatg
     7741 actttaggag acttacaaca cgttcaaaaa tactttaaag atacagaaaa gagaaatcca
     7801 actataacag aaataaaagt attagatact tattggtctg atcattgtag acatactact
     7861 tttatgactg aaatagaaaa tgtaaagata gaagatggta agtttaatga tatagtaaaa
     7921 gaagcgtatc aaatgtatct taactcaaga gacaatgtat atgtgaacag acataaagat
     7981 atatgtttaa tggatatagc cactgttgca gtaaaagaat taaagaagaa tgggaaatta
     8041 aatgacctag atgaaagtga agaaataaat gcatgtagta taaacgtaga tgttgaagtt
     8101 gatggtaaga tggaaaaata tctagtaatg tttaaaaatg aaactcacaa ccatccaact
     8161 gaaatagaac catttggtgg agcagctact tgtttaggtg gagctataag agacccatta
     8221 tcaggaagaa gttatgttta tcaagcaatg cgtgttacag gaagtgctga cccaagaacg
     8281 actttagaag atactttacc tggaaaactt atgcaaagaa aaataactac agaagctgct
     8341 catggatata gttcatatgg taaccaaata ggtcttacaa ctggacaagt tgcagaagta
     8401 tatgatgaaa actttgttgc aaagagaatg gaaataggtg cagttatagc agcagtacca
     8461 aaagagaatg tagtaagaga aagacctgaa gcaggagatg taatagttct tcttggagga
     8521 aaaacaggaa gagatggttg tggaggggca actggttcat ctaaagaaca tagtgaagag
     8581 tcaatcttaa cttgtagtgc agaagttcaa aaaggagatg ctcctaacga aagaaaaata
     8641 caaagattct ttagaaataa agaagttgct caaatgataa aaagatgtaa tgattttgga
     8701 gcaggtggag tttgtgttgc tataggagag attgctgata gtttagatat aaacttagac
     8761 ttagtaccta aaaaatacga tggacttgat ggaactgagc ttgcaatttc agagtcacaa
     8821 gagcgtatgg cagtagctat aaaaaaagaa aataaagata aatttataca attagcagta
     8881 gaagaaaact tagaagctac acatgtagct acagtaacag atacaggata tttaagaatg
     8941 ttctggaatg gaaaagcaat tgtagatata aatagagagt tcttagatac aaacggagta
     9001 aaacaaacta ctgatgtcca tgttactaaa gtagatgaag aaaatacatt ctttagctct
     9061 aatgaaatag taaaagatgt taagtgtgca agtatgaaag acaagtttac aaaagtatta
     9121 tctgacctta atgtttgttc tcaaaaagga ttagtagaga tgtttgataa tacaattggt
     9181 ggtaatacag tacttatgcc atttggagga aagtatcaag cgactccaac acaaggtatg
     9241 gttgctaaga taccagtact tggaggagaa actaatactt ctacaataat gacttatgga
     9301 tataatccaa aggtaggaaa atggagtcca ttccatggtg ccttgtacgc agtagtagaa
     9361 tctgtgtgta aattggttgc tattggagga aattatagta caacaagact tacattccaa
     9421 gagtattttg aaaaacttgg taacaatcca gaaaaatggg gtaaaccatt ctcagcactt
     9481 ttaggagcat tttatgcaca aagtaaattt gagatacctg ctataggtgg aaaagacagt
     9541 atgtctggta catttaaaga tatcgaagtt ccaccaacat tagtttcatt tgctgttgat
     9601 acagtagatg cgaagaaagt agtatctcca gaatttaaaa aagcagactc aaaagtagta
     9661 atgttatgtg taaacaaggc agaaaatgat gttgtagatt ttgaggaatt aaagagaaac
     9721 ttagacaaag taagagaact tatacatgga aataaagttt tatcaacata tgcattagga
     9781 tttgcaggtg taggtgaagc aataagtaaa atggcttttg gtaataaaat aggatttaag
     9841 tttagtgaag aagctgaaaa aacatttaca gatgataaat tatttgaggc tagttatgga
     9901 aatatagtac ttgaattagc aaatgatgat ttatgtatgt tagaaggata caattatgtt
     9961 gtgcttggaa gtacaactaa agaagctagt atatttatca aaggtgaaga acttgcatta
    10021 gatgaattat ataaagcaca ctgtagtact ttagagccta tattcccaac taaaacagaa
    10081 gaagtaaaat caaagataga gactataagt tatatatcac aaggagaagc taaaaaatca
    10141 tcattaagta tagctactcc aagagtattt ataccagcat ttcctggaac aaactgtgaa
    10201 tatgactctg caagagcatt tgaaagagta ggagctaatg caaacataag agtattcaag
    10261 aatttaactt ataaagacat agaagattct atagatacaa tagtaaacga aataaaatct
    10321 tctcaaataa taatgttacc aggtggattt agtgctggtg atgagccaga tggttctggt
    10381 aaatttatag ctactgtatt tagaaatcct agagtgcaag aagctataaa tgaattttta
    10441 actcaaaaag atggactaat gttaggtatc tgtaatggat tccaagtatt aattaaatta
    10501 ggattagttc catatggaga aataagagtt ccaagtgaaa gtgctccaac acttacttac
    10561 aataatattg gtcgtcatca agctaagatt gctagaacta gaatttcatc taataaatct
    10621 ccttggttag ctcaaactaa tgttggagat attcataata tagcaatttc tcatggagaa
    10681 ggtaaatttg ttgctagtga agatgttatg agagaattaa ttgctaatgg acaagtagca
    10741 actcaatatg ttgactttaa taatgaagct acttatgata ttgagtttaa tccaaatggt
    10801 tcattctatg ctgttgaagg tataactagt gctgatggta gagtgtttgg taaaatggga
    10861 cattctgaga gaattggtga ggaagtattt aagaatatta taggtgaaaa agaccaaaag
    10921 atatttgaat ctggagttaa atattttaga taaagctttg atgtttaatt gatagctaga
    10981 atatataatt tagatattta gatttaaaga gatgtaataa tatgatgata atcatttaat
    11041 attacatctc ttttttattt atctaagaaa taataagttc aacctaagtt aaaatacaag
    11101 ctgtgttata attaattact aagtaaaatt aaatatttat tggagggcaa cataattaat
    11161 gaaaatatta ataacagggt ctaatggtca attaggaaaa gaattggtta gccaattaga
    11221 agcaatcaat caaagtataa atcaaccaaa atacgttata ttagctacaa caagaaatga
    11281 tttggatata tccaatcaga ctaatataga taattttatt ttgcataata aacctgatgt
    11341 aattgtaaac tgtgcagcat atacaaaggt tgatgcatgt gaagataata tcgaaattgc
    11401 gtataaaata aatgccttag gagtgagaaa tttagcaata gcatcagaaa aagttaatgc
    11461 taagcttgtt cacatatcaa ctgactatgt attcaatggt ttttctaaat atccatatag
    11521 ggaagacaat aaaactgaac ccaattctgt gtatggaaaa agtaaactta tgggagaaaa
    11581 gtttgttgaa caattctcac ataagtactt tatattgaga acagcatggt tatatggtaa
    11641 tgggaataat tttgtaaaga ctatgataaa actttcttta gaaaataaag aggttaatgt
    11701 ggttaatgac cagtttggaa ccccaacaag tacagtagac ttagcaaaaa ttataataag
    11761 aatcatggaa acagaacatt atggagttta tcatgctaca tgtgaaggtg agtgtagttg
    11821 gtatgatttt gccaaaaaga tatttgaatt aaaaaatata gatataaaag taaatccaat
    11881 taagagtaac gaatttaaat gtaaggctca aagaccacta tattctgtat tggataattt
    11941 tatgttgaag ttaattggtt taaattcttt tagaaagtgg gaagaatcca ttgaagaata
    12001 tttagaaaga tatgatataa atgaaaagta aatgtattat ttttagaaaa gaaaataaga
    12061 tattataaaa aatataaata tttcttttat ttatccgata atcataatat gacaaaaaca
    12121 aatttaagga tggaaaaaga tgaaaggaat tattttagca ggagggtctg gtacaagact
    12181 atatcctata acaaaatgta tatcaaaaca aacacttcca atatacgata aaccaatgat
    12241 atattaccct atgtcggttc ttatgttggc agggatacaa gatatattaa taatatctac
    12301 accaagagat ataaaagcat ttgaagaatt atttggagat ggaagttcac tagggcttaa
    12361 aataacatat gcaattcagg agaaagcaaa tggaattgca gaagcgttta taataggaga
    12421 agaatttata ggaaatgaca gagtgtctct tgtattgggg gataatatat tttatggata
    12481 tggatttagt gaaaggttgg agaatgcagt taaaagaaaa ggagcaacta tatttgggta
    12541 tcatgtaagt aatccaaatt cgtttggagt tgtagaattt gataaggatt ttaatgtatt
    12601 atcaatagaa gaaaaaccta cagttcctaa atctaattat gctataccag gattatattt
    12661 ttatgataat gatgttgtag agatagcaaa gctagttgaa ccatcagata gaggtgaatt
    12721 agaaataacc tctataaaca gtgaatatct aaaacgagga aaactaaaag tggaacttct
    12781 aggaagaggt atggcttggc ttgatactgg tacacataaa ggtctattgg atgcttctaa
    12841 ctatgttgaa gtggttcaaa caagacaggg actgtatata gcatgtttag aagagattgc
    12901 atatacaaaa ggatatataa gtagagaaca attaatgaaa ctagccaagc cactgataaa
    12961 aactgaatat ggtcaatatc tattaaaatt ggctagtgaa gatatgcaat atggtagata
    13021 aaatttatta taaattttat taagggagtt tgttataatg ggaaaaatca atgttataaa
    13081 gactaaaata gaaggattat atatagtcga accaaatgta ttttgtgatg aaagaggata
    13141 ttttatggaa agctataata aaaaggattt tatagagcag ggtcttgata tgtgttttat
    13201 tcaagataat gagtctagct caagaaaagg tgttttaaga ggaatgcatt ttcaggttag
    13261 atatcctcaa ggaaaattag tcagagttac agagggagaa gttttcgatg ttgcagttga
    13321 tttaagaaaa gattcacaga catttgggaa atgggaaggt gtaatattgt ctgataagaa
    13381 taaaaaacaa ttttatattc cagaagggtt tgcacatgga tttttagttt tatcagaaaa
    13441 agcagtattt aattacaaat gtacaaacta ttattatcca gaatttgagc atgggtttat
    13501 atggaatgac aagcaaatat ctattgagtg gcctttaaaa ggaattgaag aaatattttt
    13561 atcagaaaag gacaaaaaac aaaaaagttt taaagatatt tgtataagac gttaataaaa
    13621 atttattaga attaacattt aggagaaaaa atatgaaaaa aatactgata actggaggag
    13681 caggatttat tgggagtaac tttgtacatt atatgcttaa caagtatgta gattatttta
    13741 ttgttaacct agacttgttg acttatgctg gtaatttaga gacattaata gaaatacaag
    13801 acagttccaa ttataaattt gttaaaggag atatttcaaa tagggaacag atgtataagc
    13861 tatttgaaga agaaaggttt gatatagttg ttaactttgc cgctgaatct catgtagata
    13921 ggtcagttga gaatcctgat ttatttatta aaacaaacat tataggaaca caagtacttt
    13981 tagatgcatc tataaaatat ggagttaaaa ggtatcatca aatatctaca gacgaagtgt
    14041 atggtgattt accaattgat agaaaggatt tattctttac agaacaatct cctatcaatc
    14101 caagttctcc atattcagct tctaaagcat cagcagattt gttggtaagt tcttattata
    14161 gaacatatgg attgttaaca actatatcta ggtgttccaa caattatggg ccatatcatt
    14221 ttcctgaaaa attgatacct cttatgattt taaatgcttt ggaaaacaaa caattgccag
    14281 tatatgggaa tggggaaaat gtaagagatt ggttacatgt atatgaccat tgtactgcta
    14341 tagacttaat aatacataaa gggaatatag gggagattta caatatagga ggtcataatg
    14401 aaagaagtaa tttagaagta gtaaaaatga tattaaatct tttagataaa tctgaagagt
    14461 taatatccta tgtaaatgat agacctggtc atgatttaag atatgctatt gatgcaacta
    14521 aaatagagaa tgagttaggt tggaaggcaa aatatgactt tgatttgggt ataaaagaaa
    14581 ctgttaagtg gtatatagaa aatgaatctt ggtggaaagc tgttttatca gtgtaacttt
    14641 gaaatttata aaatttagaa aggggcgttt tatattgaat gagataagtt cactaattaa
    14701 tatattagca ttgactcaat tatctggtag taatactaca aatcaatgta actgtacata
    14761 taatagttca tctggatttg atatgattat gatgaccttg ttaaaagcat tatcacaaaa
    14821 taatcaacag acttctaatg gctacaattt atcgtctaat tctgagaatt tatttgacga
    14881 actcggcagt gtagtaaata atacagctag tagatttata gatgttgata caaaagataa
    14941 aagtgtaaaa tcaagaattg aaaatgctat agagcaagca tcaaaaaaat ataatgtgga
    15001 ttcgaatctt ataaaagcaa taattaaagt tgaatcggat tttaatccaa atactgtttc
    15061 ttctgctggt gctaagggtc ttatgcaact tatgccagaa aattgcagag acttgggggt
    15121 tacagaccct tttaatatag agcagaatat agatgctgga actagacata ttaaggaata
    15181 cattgatatg tttggaggga gcatagaaat gggattgatg gcatataatg gtggtcctgg
    15241 aagaatgaaa agtagaggag tagaatcaat ctctgacctg tataaaatgc caaaagaaac
    15301 acaaaattat attcctaagg ttatgaaata ttatagaggg taggctttat tatggggata
    15361 aatatagata agctaaacat aagtccaagt ataaatatgg aggttaaaaa actaaaagag
    15421 agtaataaat tttctgacaa attaaactct ttaaaacaat cttcttcatt ggaagatata
    15481 aaacgaaatt tggaaagtgt aaaaaaggca ggagaaaggc ttgtacttac tcaaaactat
    15541 ggcgacatta gtatatataa aaatgcagta aaagaatacc taaaatctgt tgtagataat
    15601 atgtattcac ttgataaaaa tagtagcttt tgggagcatc agtattataa gaatgtaaag
    15661 attgttgata gtaaacttga ggatatgact agtaagttaa ttagtgaaga aaaagaaaac
    15721 atagatattg tttctactgt tgatatgata caaggcttgt tgatagatat gtatagataa
    15781 ggttatacaa ggagaaaagt tttatgaata gtaaaaaaga ggatatatat gaattgaatt
    15841 ttgggcaatt aatagaagaa aaatctggtg ttatagaaga aaatcaaaat gtttttaatg
    15901 caaaagtaaa tgtagctgtt tctataggaa atacaacaga aacaataaaa aatatattag
    15961 atttaaaaga aggctctata ataaaactaa gtaagaatgt ggatgaaaaa ttagatatat
    16021 atgcaaatga tagattgttt gcttatggag aaagcattat tacaaatgag aagttatctg
    16081 taagattatc taaaatagaa gattcagatg taaattagta ttttaaatag tatcccttta
    16141 ggctatgatg tatgagttta gctaagggga tatttttata aaaaatttaa aatatgcgtg
    16201 aattatataa atgtttttat tttatgagcg ataattaaat tataagtatg taattttgga
    16261 ggtgaaacta tggatattaa aaatgtaagt tcaaatattg tagtgaataa ttatttaaag
    16321 aatacatctg gtgtaaaaaa agtggaagaa gttaaaaaaa ttaataaaac agacaagata
    16381 gagctatcta aatttgcaaa ggaaatggca agtgtgtcaa atatgagtga tgagaatatt
    16441 aggaataaaa aagtcgatga aataaaaaat gcattagaaa atggaactta caaaataaaa
    16501 ccaaaagaaa ttgcgaaaaa aatgatagag gatatgcgag gataaagtca tgattacaga
    16561 cttaaaagtt ataatatatg aagaaaaaaa tataattagt aagatgatgg aattattaaa
    16621 agaacaattt gattatatag ttgaaaaaga cttagaaaat ttaaatatgt taaatcctaa
    16681 actagaagat attagcaggg agcttgcatc catagaaata aagagaagac aactttttgg
    16741 tgatgatatt tcaatgagtg aagttgttga aaatagtaat gatagatatt taaaagaaat
    16801 atatacagac ttgaaacaca ttcttgcttt agctaaaaat caacaagaat caaatgattc
    16861 tctaataaag aaagaactta tttttacgaa aaaaatgatt aatttcataa aaccagctga
    16921 taagcaaact gcaacttata attcttatgg aaatattaga aaataaatat taggaggaga
    16981 aatatggcag gattattttc cattttaaat acagctaatt caggaatgaa tgcgcaccaa
    17041 aaagcaatac agacaatttc tcataatata agtaatctag atacagacgg ttattctagg
    17101 caaaggactg agtttgctac aaatagtcca atgtatatgc ctagtttgag taattcaatt
    17161 gcaagaggac aattagggac tggagtacac gtaacggatg taacaagagc tagaaatagt
    17221 ttttatgatt accaatttag agcagaagct cataagtatg gtaatacagt ttcaaagtat
    17281 gactattata atactataga aactataata aatgaaccat cagactatgg aatatctgca
    17341 ggtatagacg acttttttaa aggatgggaa tcattagcaa aggatataaa tagtgatagt
    17401 aaaaaaagtc tagtggtaga aaatgcatca aatctagcaa ctttaataag tgaaagttat
    17461 aagaagctta ataagttaaa gggagatata gacgataata taaactctga aataaaacag
    17521 ataaatgaca tgttaaaaag tcttgaagat ttgaataaat ctattgatgt tatatctggt
    17581 tctggctcta ctccaaatga tttacttgat gaaagagata gaatattaga tgacttaagc
    17641 tttaaattag accttgaaaa tagcgatgtt aaaaatatgc tttcagatgg taaattagaa
    17701 cttaatgaat taaaaaatgc agatggaact tggaaaacag gtgtatcagg aaccttacaa
    17761 ggcttgtttg aaatgcatgg taagattgat acatataaaa gcgatttaaa agatgtatct
    17821 gatgggttag ctaagcagat aaatgatgtt tataatacat ctgctggaat aacagtaaca
    17881 gattttttta taacttcaaa tgtagatggt gaagatatca taaaggtaaa tccagctata
    17941 aagagtaacc caaatgaatt aaaacttact gctgatgagg cttcaaagat tgctaagtta
    18001 aaggatgaaa aaatagatat tggggtggct ggtggaaaag taagtacaat aagtgaccac
    18061 tataaagcct ttgcagaatc agtaggttta gattctcaaa aagtgaatca agatgaagtg
    18121 aatcaacgta aaattataaa taatattgat aactcacgaa tgagtgtttc aggcgtatct
    18181 ttggatgaag aaatgacaga gcttataaaa atacaacgct cctatcaagc tagtgcaaaa
    18241 gttatgtcaa cagctgtgca actgttggat gttgtaataa atggaataat ttaatagaag
    18301 ggggaattta taatgagagt tagtacaggt atgatgtcta gcaactatct aaactcacta
    18361 caagataatc tacaaagatt agataaggtt aatagacaag ttaatacaac gaaagaaata
    18421 aacaagctat cagataatcc atataaggca ataaaaatat tgaactcaaa aagtgaaata
    18481 aaaactatgg agacttatgt tgagaattgt aaagatacag ggaattggtt agaaactact
    18541 gatactgcat tagatcagct tggaaaatta ttatcggaca taaagaaaac tttggtggca
    18601 agtggtgatg gtgcatattc agatgatgag ctaaagactc ttagtaatga aactaatgaa
    18661 aagatgaaag aaattgcaaa tgccttaaat gcaacacatg aaggaaagta catattttca
    18721 ggttcaaata caggtgtacc acctgttgaa tgtatagata atgctgacgg ttcagtgagc
    18781 ttaaagttta attctagttt aaatcttaat aaattaaatg actctttgag tatggatata
    18841 gctcaaggaa taagtgtaga ttataatgtt aaattgagta gccttggatt tgacccaaca
    18901 aagactgacc catttgaaga gttaaataat atatcaaaga aattagcaaa tccagatgct
    18961 gatgctataa aagagctgac aacaacttgt ttaggcgaca tggaacaatt aatagaaaat
    19021 acagtaaatg ttaggtctat atatgggaca aaagcaaata cagtagaggc tatgaaggaa
    19081 aaaaatgatg atagtttgat acagttaaag gatattttgt cacaaaatga agaaatagat
    19141 tatggagaaa aactcgttca attaaaggca gcagagttaa cttatcaagc ttcactacaa
    19201 acaggaggaa agttatttaa tgtatctata ttagattaca tatagtaaaa taacgcagat
    19261 atgatgaagg ttacattaaa aaaaggtata ttaggttttg aaaatttaaa agaatatgag
    19321 cttttagata tagaagataa tgattcttta aaagaattta attctactga agaagaaggt
    19381 ataggatttg ttgttgtatc tccatttgag attatcaatg aatatgaaat agttttaaat
    19441 caggaaacaa tagaaaaact agaagtgaaa tctccaaagg atgttgtact atttaatata
    19501 attactcttg gtcagacatt tgaagaatca actgtgaaca tgaaagctcc tatagtgata
    19561 aatataaaaa ctaattttgg aatgcaaata attttacagg atgataaata ttcaataagg
    19621 catccattat tgagaggtga taatggatgc tagtaatttc aagaaaaaaa gatgaagcag
    19681 tacttatagg agaaagcata gaagtaaaaa ttgtaggggt agatggaaac aatgtgaaac
    19741 tagctatatc ggcgcctaac aatataagta tattaagaaa agaaatatac gaaaaagtaa
    19801 agagtgagaa tataaaagct acaaataaaa atataaaaat tttaaagtca ttaaaataat
    19861 aagcttggag gaagtaatat atgtatggag aaaatcctta taattcttat aaacaaaatg
    19921 ctgtttttat ggcatcaaag gaacaattgt tacttatgct tgttgatgga gcagtaaaat
    19981 ataccaaaat agccagagca gctatcttag ataaaaatac acagaaagcg catagagaac
    20041 ttgtaagagt tcaggatata tttacagaac taatggttac attagaccaa aatgcaggac
    20101 aatgggcaaa ggatatgtac aaagtttatg attttgtaag atatgaactt tcaagagcca
    20161 atattagaaa agacatccag gtaatagata atgtgttgcc tgtaatagaa caaattaaag
    20221 acacatggca tgaagctgat aaaaaaagta aagaagaaag aagtaggtac aaataatgga
    20281 attaaattta gctgaattag ataagttatc aaaagaggaa ttactattga tgcttgtaga
    20341 tggcacagta aaatatacca atatatccaa agaagctctt ttaaataacg actatttgaa
    20401 agcacataat gaacttataa gagttcaaaa tatatttaca gaactaatga ctacattaga
    20461 ccaagatgca ggtcaatggg caaaggatat gtataaagtt tatgaattta taaaatctga
    20521 attagcaata gctgatgaaa ataaagatat taaaataatt gatgatatat tgccaatagt
    20581 aaaacaaatt agagatacat ggcatgaagt ttacaatcaa ctaaaaatat aattagatgg
    20641 agggataaag gtatgtcaag tataagtcct ataagagtta caggtctttc aggaaatttt
    20701 gatatggaag gcataatcga agctagtatg attagagaca aggaaaaagt tgataaagca
    20761 aaacaagaac aacaaatcgt taaatggaag caagaaatat atagaaatgt tatacaagaa
    20821 tcaaaagatc tttatgataa atatctaagc gtaaattctc ctaatagtat agtaagtgaa
    20881 aaagcatact cttctacaag aataaccagt tctgatgaaa gtattatagt agcaaaaggc
    20941 tcagctggtg cagaaaaaat aaattatcaa tttgcagttt ctcaaatggc tgaaccagca
    21001 aaatttacta ttaaattaaa ttcaagtgaa cctattgttc gacagttccc tccaaatgcc
    21061 agtggagcta gttctttaac tataggagat gtaaatatac caatatctga acaagatact
    21121 acaagtacta ttgtaagtaa gataaactcc ctttgcgcag ataatgatat aaaggcttct
    21181 tatagtgaga tgacaggtga attgattatt tcgagaaaac aaactggttc gtcatcagac
    21241 attaatttaa aagtaattgg aaatgacaat ttagctcagc aaattgctaa tgataatggt
    21301 atcacatttg caaatgatgc tagtggaaac aaagtggcaa gtgtatatgg aaaaaatcta
    21361 gaagctgatg taactgatga acatggaaga gtaactcata taagtaaaga acaaaattca
    21421 tttaatatag ataatattga ctataatgta aattcaaaag gaactgcaaa gttgacttct
    21481 gtcactgata ctgaagaagc tgttaaaaat atgcaagcat ttgtggatga ttataataaa
    21541 ctgatggaca aggtctatgg tttagttact actaaaaaac caaaagatta tccgcctctt
    21601 acagatgccc aaaaagaaga tatgacaact gaagaaatag aaaaatggga aaagaaagct
    21661 aaagaaggta tacttagaaa tgatgatgag ttaagaggtt ttgttgaaga tattcagtct
    21721 gcattttttg gagatggaaa aaatattatt gcattaagaa aactaggtat caatgaaagc
    21781 gaaaattaca ataaaaaagg tcaaatatca tttaatgcag atactttttc aaaggctctt
    21841 atagatgata gtgataaggt atacaaaaca ctagcaggtt attcttcgaa ttatgatgat
    21901 aagggaatgt ttgaaaagct aaaagatatt gtatatgaat attctggaag ttcaacttct
    21961 aaacttccta aaaaagcagg tatagaaaaa actgcttctg ctagtgaaaa tgtatattca
    22021 aaacaaattg cagagcaaga aagaaatata agcaggttag ttgaaaaaat gaatgataaa
    22081 gagaaaagac tttatgctaa atattcagcc ttagaatctt tgttgaatca gtattcttcc
    22141 caaatgaatt atttctcaca agcacagggt aattaattat ggataaatta aaagaaaaat
    22201 taaatttata taaagatata tctttacaaa ttataaattt gattgaaaag gaagaatata
    22261 taaagataag tagtaagtta ggtgaaagac aagagattat aaatagtgta agtgaaattg
    22321 atagaaatga ttttatacag ctttataata gaatggaatt aattgaaatt gatagtagga
    22381 taagagatat tttacaagga caattattag aagtaaaaaa agaattacat gagtataagc
    22441 ttactaaaca agtgaacaca atgtattata acttaaatag agaaaaagta aatattttta
    22501 ataaaaaagt ataaagttat gtaccatctt gtccgattat ataaatgaag aattaataaa
    22561 aaagattaaa agtagaaatg acaaggatgt caactatact aaggagggta aaataatgag
    22621 agttaataca aatgtaagtg ctttgatagc aaataaccaa atgggaagaa acgtaaatgc
    22681 acaaagtaag tctatggaga agttatcttc tggtgttaga attaagagag ctgctgatga
    22741 tgctgctggg cttgctatat ctgagaaaat gagagctcaa ataaaaggtt tagaccaagc
    22801 aggaagaaac gttcaagatg gtatatctgt tgtacaaaca gcagaaggtg cattagaaga
    22861 aactggtaat atactacaaa gaatgagaac tttatcagta caatcttcaa atgaaactaa
    22921 tactgctgaa gaaagacaaa agatagctga tgagttgtta caattaaagg atgaagttga
    22981 aagaatatca agctcaattg agtttaatgg taagaaatta cttgatggaa gttctacaga
    23041 aataagatta caagttggag ctaattttgg aacaaatgtt gcaggaacaa ctaacaacaa
    23101 caatgaaata aaagttgctc tagtaaatac ttcaagtata atgagtaaag caggtataac
    23161 aagttctaca atagcaagct taaatgcaga tggaacttct ggaacagatg cagctaaaca
    23221 aatggtgtct agcttagatg tagctcttaa agaattaaat acatcaagag caaaattagg
    23281 ggcacaacaa aatagactag aatcaactca aaacaactta aataatacta tagaaaatgt
    23341 tacagcagct gaatcaagaa taagagatac agatgttgct tcagaaatgg ttaacttatc
    23401 taaaatgaat atattagttc aagcatcaca gtcaatgctt gctcaagcta atcagcaacc
    23461 acaaggagtt ttacaattat taggataaaa taattttgta tattggatat agaagaaagg
    23521 gtaaggcttt gctttactcc tttctttttt tacaaatata cttttgaaag gaaaatatat
    23581 atgttactta gtatagttat gattgttaaa aatgaagaaa aaatacttga aaaaacttta
    23641 aaatcattaa ccacattaag aagttctata gagagtgaac ttataatagt tgatacaggt
    23701 tctacagatg atactataaa tatatctagg aaatacacgg aaaatgtgta ctttcataat
    23761 tggaataatg atttttctag tatgagaaat atatcaatat cctatgctaa aggtgaatgg
    23821 ttacttatat tagatgcaga tgaaatacta attgattctt caactattgt gaaatttttt
    23881 aaagatggtc ttgataaaaa atttaattcg gcctcaatac aattaaaaaa tctttactca
    23941 tatgataaaa aattatatgg atattgctca gtgcttagac tttttaaaaa catcgatttt
    24001 agataccatg gtaaagtaca tgaacaacca atatacaaaa accctatatt taataatgtt
    24061 gctgattttg aacattatgg atatttgttt gaagatgaag aaataagatt aaataaagta
    24121 aaaagaaatg aaaaactgtt atttgaagaa ttaaaagaga atgataattc tccatataca
    24181 aattatcaat tagggaaaaa ttttattata ttaggaaaat atcatgaggc tttatactat
    24241 cttgagaaat cttatgaatt atattctaaa ctagaatcgg cacctggtta cttgataaca
    24301 agtcttgtaa aaacatattt atatttagga aagcataaga agtgtgaaaa attgtgtcta
    24361 aagtatatta agaaagacat gaataatatt gatatatatt attatattgc acaagcacaa
    24421 gtagatttag ggaagtatga aaatagtata gattcgtata agagatatat ttatttgtta
    24481 gataattatg aaataagtac acaagcaaat agtttatttt cagacactga tactgtaggg
    24541 cttagggatg aagctattat tacactgata aagatttatt ataaacttga aaaatatgag
    24601 tctgtaattt cagagtacaa taacattgag gatatggaaa agaagaagaa tgtgtacttt
    24661 agcttattta tgtccctata caaattgaac agatttgaag atataaaaaa ctattataaa
    24721 gaagtttcaa attctaaaat tgaaaaaaat agtttttata aaaatataga agaagttata
    24781 aataatatta aagatgatga aaaagagcat gtatataaaa tcctttgtga cattgaaggt
    24841 aattatggca ttttaaatca gataaggata aataaatgtg tatctataga taagtgtaaa
    24901 gaaatactca atgaagaaaa agaaataatt tatgcatata ttataaaagt agcttttgaa
    24961 gataagattg atttactaga tattttttat gaaatggaat atgtatggat agagaaatac
    25021 ctaaaatatc ttttagcact agaaagaagt tttaatttag tactatataa gtacataatt
    25081 agtcgtccga atacatcaga tataaaaaag ataagagtat ataaagctat atctaaagta
    25141 gttttagaaa gtataagttt aagtgatgaa aaatataagg agatttttta tctttatata
    25201 atgtattctt ataagtacat aagatacata tatagaaatt taagcgattt tgaattaata
    25261 aaatttgttt gcaatgattt agataagttt actatagaat ttaagcatct acttgacatt
    25321 aaaaataaag taaatgatgc taatgaaaaa ttagagtata tacatagtat gaggaaatta
    25381 ctaaatgagt atccttttta taataaaata ataaaaatac ttataagtga attagaagaa
    25441 aatataaaag aaagtgaaga atttaaggat ttaaaagata gttttattat tagtatagaa
    25501 aatatgattg aaactggcaa aataaatgat gcgaaatcat tggtagatga ctattcaaaa
    25561 acatttatgg atgatgtaaa aattttaaat ataaagggaa ttttatatat gtttgaaaat
    25621 aagcttgata aagctgattt tatgttaaaa aaggcattat cattagatgt agaaaatgaa
    25681 gatactatat ataatataga atacttaaaa agtttaagat aagtcaaaaa tgggagaatt
    25741 gaagaatgaa tacaccaata gtggttaatg aaaaatctaa agatataata aaagattggc
    25801 aatatacgta tacaaacgaa gatataaaat ttacaagcat aataatattg acatataatc
    25861 aaatagaata tactaaatta tgtattgaaa gtattaggaa gtttacccca aagaataaat
    25921 atgaaataat aataattgac aataactctt ctgatggaac agttgagtgg ttaaagggac
    25981 aatctgacat aaaatctgta tataataaca aaaacctagg atttccaaaa ggatgcaatc
    26041 aaggtattga gttggctact ggtgaaaaca tattgttgtt aaataatgat gtaatagtta
    26101 ctccaaattg gctttataat ttgaataaag ctttatggtc tcaagataat ataggtgctg
    26161 taggtcctat tacaaatgca tgttcgtact atcaacaaat tgatgttgat tataaatcaa
    26221 ttgaggatat gtttatgttt gcacacaaaa taaatacatc taatattaac ctatggaaat
    26281 atagattaaa gttagttgga ttttgcatgt taataaaaaa atctgtgctg gataatgtag
    26341 gggttttaga tgaattattc acacctggaa attttgaaga tgatgattta tcttttagga
    26401 tgctagagtc aggatataag ctattattat gccaggatac ttttatacac cattttggaa
    26461 gtgtatcttt taaagaaaat cgtaataaat atacagatat aatgtatatt aatagaagaa
    26521 aatttgaaac taagtgggga tttaatccag aatatagtgg aatgataaga acagatttga
    26581 tttctaaaat agatgatagt ttggaggcaa atattaatgt tttagaaata ggctgtggta
    26641 taggggctac tcttttagaa ataaaaaata tatataaaaa tgcatcaata tatggaatag
    26701 aaattagtga gtcagcaggt aaacttgcaa agtatagtgc agatgtactt atagcagata
    26761 tagaaaaagt aaagttggat tatgaaaaag atttttttga ttatataata ttgggagatg
    26821 tacttgaaca tttaactgac ccatggaaga tagtcaatga actgaaacaa tatctaaaga
    26881 aggatggttg tttgatatct agtataccaa atattatgca tgtttctgtt ttaagaaacc
    26941 ttgtgttagg taattttaca tatgaagatt caggaatttt agacaagact catttaaggt
    27001 tttttacatt aaatgagata acaaaaatgt ttaatgatag taattacata ataaatagtg
    27061 ttacagctac tagtatgcca ctttcggaaa ttgataatag attcattgat acaatgtgta
    27121 aggaaagctg taaagaaatg agacaacagt acgaatctta tcaatatata gtaaaagcca
    27181 ctaaaagcat agatactaaa agatattcta gtattagtat ggttaattta aaatacgctt
    27241 taatgagaat tgataatgaa ttagatgtag atagtaattt aagttatata tttgataatt
    27301 ataatataac tgaaccatgt tttgttgatg atattgaata tttagttgat acgtttgtga
    27361 taaataaagc aactctatta aatagattag gagttgaggc atttaacaga ggtttaggag
    27421 attttagtat aaacatgttt atgacagtat tagagatgga tagagatgat attgattcaa
    27481 tatataatat aacaagtgta ttatgtgaat tagaagaatt tgatactgct tacaacttaa
    27541 ttaaaaatag tagcaaaaaa gtacagagtg aaagtgacat acaagatata ctttatttta
    27601 ttgaggggaa gagatatgaa taatgataag atatgtttta taacctgtgt aaatgatgaa
    27661 agactttatg aggaatgtct tgtatatata aatagcctag acattcctaa aaactttagc
    27721 atagaaacag tagctttaag aggagccgat tctattgcct ctgcttataa taaagctatg
    27781 agagaaacag attctaagta taaggtatac ttacatcaag atgcttttat aattaataaa
    27841 aatttcatat atgacatttt agagatattt aaaaaaaata cagatgtagg aatgattggt
    27901 gtagctggag taaaagatat acctgtgaat ggaatttggt gggattctga taaaaaagca
    27961 gggaaagtat atgatagcca tacaggtaaa atggatattt atgaatttag taactttgaa
    28021 gaatgtttta atgaagttat ggctatagat ggattaatta tgattactca atatgatgta
    28081 ttttggagag aggataagtt tgatgggtgg catttttatg atttgtcaca gagtatggaa
    28141 tttagacaaa ataaatacaa agttattgta ccaaaacaaa ataagccttg gtgtatgcat
    28201 gattctggag tagcaaatat aaataatgga tatgatgaat ataggataaa atttgtaaaa
    28261 gagtacataa atcttgattt atatagagaa aatttttatg aatttgataa gaagaaagca
    28321 agtaccatac ttaagagtaa taagcatgta atggattgta tgaaagagga gcttacagaa
    28381 caattaagat taaagaatta taaggatgtt ttagatattg tttctaaaat tgctcatttt
    28441 tctgttttta atcatactgg cttgtattct tgtgaaaaat gtgagagagc attgctagaa
    28501 tgtgcaaata atttaccaga aatagaaaat acgaaattat ttacaaaaaa agatttaaaa
    28561 agaagaaaag tattacatgt tttaagtgaa ggatattcaa caggagggca tacaagacta
    28621 gttaaaaatt ggattgagat tgacaaagat tctgttcatt cagttgtaac tacatggcaa
    28681 tcagatacac tgccagactg gttattaaaa gcaatagatg atagtggtgg cgaagttata
    28741 tctttggatt taatttcaga tgattattta aaaagagctg tagctttgag gaaaatagct
    28801 tatgactggg ctgatatagt tattttatat gttcatatgt gggatacaat acctgttgtt
    28861 gcttttggag ttgatgggaa aatacctatt ttacatgtta atcactatga tagtactttt
    28921 tggttaggga caagtattgc agatattgtc attgatttta gcgaagctag tcaattaatt
    28981 acattagata aaagaggcat agataaatct gaaatattac caatgccatt aaaagaaaaa
    29041 gaagtatttt caaaatctga agttagaaaa cgatataatt tggaggatga tgtgacaata
    29101 atattgacta ttgctagtga aagaaaatac aatagtgtaa atgatattaa ttactttaaa
    29161 attgctaaaa cattgattga aaatacagat aatacaatta ttattgttgt aggagttgat
    29221 acagagaatc tctattggaa aactttaagt aacgttacag ataatagagt tatatgtttt
    29281 ggaacaacag caaatataga cgatttttat aagatttcgg atatatacct agagtctttc
    29341 atggtaggga gtcatacttc aaaattagat gcaattttat atgatttaat tcctgttaag
    29401 tttaaaaatg aagtttcacc aatttttaca aatatgtgga attttataga taagtttgat
    29461 tataagaata taaaagaggt tatagacatg ataagaaggt acaatactag tgaatttgac
    29521 agggataaga taaaagaaaa acaatcagaa ataaaacatt ttgttataga taaacactgt
    29581 attgaaataa aaaactatat agacaaaata tataaagagg ctaatcatca taaagttaag
    29641 tatgatttat ataaaaatga tgaaatcgaa gactatcagc tttttgttgc attatatggt
    29701 tatatggtaa aaaataaaaa catgtcatag gtgataaaat gaacaatatt catatcaata
    29761 aatttgaaaa aagctgtgaa aaaaactggg attattttgt agaaaataaa tctataaatg
    29821 gtacatttct gcaaactaga aatttcctta attatcatga agaaggaaaa ttcactgacc
    29881 actcattaat aatatataaa ggtgaagaaa tactagcagt cgtacctgca tgtgaaactt
    29941 atgaaaatgg tgaaagagta tttttttctc atttaggaag cacttttgga ggtattataa
    30001 taagaagtgg ttttaatgat attaggcatg taaatgctat tacaaataag ttagaaacat
    30061 atttacagga acaaagtttc aataaagtat atttaaaaaa tacatcaaga atattttgta
    30121 aagaggatac ttcattacta gaatattttc tatttaagaa taattacatg ttttatgatg
    30181 aattaagtat gtttttagaa ttaagtaagt ataaaaaaga tatattagct aattttagat
    30241 atagtaaaag aagatatttt aaatattctt tgaagaataa tcttaccttt aaaagattgt
    30301 atagtgatga ggatatagtt tgttttttta atttactttc taattgttta gatagtaaag
    30361 gagctaaagt aatacattca gtagaagaac ttttggagtt taaaaataat agattaaaag
    30421 atattgtaga attttatgca gtatatattg atgacattat gatttgtggg agcatggtat
    30481 ttaactttca agaaagagtt tatcatactc aatatctagc atcaaattcg acatataata
    30541 aatattatcc aatgatattt ttatactata atctaataaa agatgcttta gagagaaatt
    30601 ttaagtatct ttcttttgga attagcactc taaatagagg caaatactta gacgagggtt
    30661 tagctacatt taaagaaggt tttggttcaa gtgcaatttg caacagaaca tactataaaa
    30721 atataaataa gtgataatca tgttttatag agtttttaga gtatagtaaa tatctagtac
    30781 tctaatatta cataaaaata attagtttat aaagaaggtt tgtatatgaa aagattaatg
    30841 attttaggct caattaagta ttttgagaat attgttatgt cagctaaaaa agagggaata
    30901 tacacaattg tttgtgataa tagaattgat actcctgcga aaaagatttg tgatgaagca
    30961 atagatgttg atgtatttga ttttgataaa ataaaaagta tagctataga taaaaagata
    31021 gatggaattt taactggatt tacagattct ttaatgaaac catatgttta tattgcaaat
    31081 gaactaaatt taccatgtgt gatttcttgc aatcaacttg aatctgtaac taataaggct
    31141 attatgaaaa aatattttaa aaataatgca atacctacaa ctgattattg tattattgaa
    31201 agtctaaaag atttagaaaa aatacaagga ttaaattttc cacttgtaat aaaacctgtt
    31261 gatggttatg gttcaagagg tgtatatttt gtatcaaata tagaagaatt aatagaaaaa
    31321 tttgaatatt ctaaggaagc ctcacaagat ggacaagtaa taattgagga attttatgaa
    31381 agtgaggaaa tacaaggact agcttgggtt cataatggag agtcacatgt attttatatt
    31441 ggagatagag agcttgtaaa tatacataca ggaagagctg gaaaaccaga tagattactt
    31501 tatccatcaa agtattgttt tcaatatgag gaagaaataa aatctatata tcaaaatata
    31561 acacaggctt ttgatataaa aaatggacct ctttatgtac aaatgttagt gggagcatat
    31621 ggaataaagg tatctgaagt aatgccaaga ttacctggag gttgtgacta tatggctatt
    31681 tctcaaataa cagatttaga cataggaagt ttatttgcaa actttagtgt gaataatgaa
    31741 gtaaattttg atgaaataaa gaaatataat atgcatttat caaaatgtgt atatgcatta
    31801 ccaatataca taagacctgg aacaataaaa aagataaata atatagataa aatagaaaac
    31861 ttagattatg tcactcaatt tctattatta gtaaaagaag gagatacaat aggattatat
    31921 gaggatgaga gacaggattg tggaagagtt tatggaatag caaataatat atttgatgca
    31981 aatataaaga agaaatgtat tcatgaaatg atagaaatat ttgatgaaaa taatgaaaat
    32041 atgatagaaa atttttaaat aagggagtat gatagatatg aagctaataa ctcatgaaca
    32101 aattatgtca ttaaacattt ctcagaaaca aggatatgaa tggataaaag aatctttttt
    32161 aacaaaaaaa gattgtcaat taccaccaaa aataagcatg gtagaagaag gtcatgtatt
    32221 ttacaatgta atgccgtcct taaatcataa ccatatgatt ggtggaataa aaatggttac
    32281 aagattgcca gggagaaatc caagtttaaa ttcagatata tttctataca atatgaaaga
    32341 tgctaaatta aaggctattt tggatggtaa ttatattaca gcttttagaa ctgctgctgt
    32401 tgctggtttg gctattgata cttttgctaa aaaagatttt aatactattt ctattatggg
    32461 tttaggtgta actgcttctg ctataatgaa tatgctttcg tatactctta aagataaaaa
    32521 tataaatata aagttactta aatataaaga ccaagcagag tcatttataa aaaagtatga
    32581 aaaaaatgaa caatttagtt ttgaaatatg tgatacacat gaagaactta ttgtagattc
    32641 agatgtagtt atttctactg tgacatatac agatgaatat ttatcagaat ttaaatggtt
    32701 taaaaaaggt gctttattat tacctgtaca tgttatggga tttggaaatg ttgattatct
    32761 atttgataaa gtatatttag atgattataa tcatttgaaa catctagaaa atatagatag
    32821 atataaatat tcttgtgaat tttataatgt tttaagtaaa caagctaaag gaagagaaaa
    32881 tgatgatgag agaataatat cttacaatat aggtttgtca atacatgata tattatttgc
    32941 aaacaaaatt tacgaattgt gtgaaacact aaattgtgaa gagatagaat taaaagaacc
    33001 aaaagaaaaa tattggatat agtttatttc attcatattt acaataattt attgtaaata
    33061 tgaatatgaa gtaatatatt gttagaaaat tagcatgaat aggatagata aatttatgga
    33121 taaatataat ttaattaagt tcttatctat tggaaacgaa tatataggaa atttagttgc
    33181 attagaatca aataaagcaa taccttttga tataaagaga gtttattaca tatatgatgt
    33241 accaaacaat gtaaagagag gatttcatgc acacaaaaac ttggaacaag tactagtttg
    33301 cctaaatgga agtgtgaaga taaaatgtta tgatggtgta aaagagagta tatttaattt
    33361 acataaaaat gatgttggat tgtatatagg aacgggagtg tggcatgaga tgtttgactt
    33421 tgatgaaaaa actgttttaa tttctatagc atctgaacat tacaatgaag aagattatat
    33481 tagagactat aaagaatttt taaagtattt aaataatatg tagttattat acagttagtg
    33541 atgtattttc ttaattaaat aaatttaaga tagccatata gagaataaat agattaggat
    33601 gtgaatttat tgttggttaa atttaataat ttattaaaac agttccaaac tcataaagaa
    33661 gaatatgaac agaagattaa ggatgtaatg gaaagtggat attatatcct tggaaatgaa
    33721 gtaaggcatt ttgaagaaga atttgctagc tatgtaggta gcaaatattg tgttggttta
    33781 gccagtggtt tagatgcact tataattgct tttagagcat taggaataag tgaaggtgat
    33841 gaagtaatcg tgcaatcaaa tacatatatt gctagtgtta tgggaatcag cataaatgga
    33901 gcaacaccag tatttgtaga accaaatgaa tattataata tagattcatc taagatagaa
    33961 gaaaagataa atagcaaaac aaaagctata ttagtagttc atttatatgg tcaagcaagt
    34021 aaaatgaaag atatacaaga attatgtgat aagtataact tgtatttaat tgaagattgt
    34081 gctcaatctc atggagctaa atatgatgga aaaatgacag gaacatttgg tgatataggt
    34141 tgttttagtt tttatccaag taaaaatttg ggtgcatttg gagatgcagg agctatagta
    34201 actagtaatg aggaaatagc tgataaagtg aaaattctta gaaactatgg ttcagaaaag
    34261 cgatattata ataaatttgt cggatataat tcaagattgg acgaaattca ggcaggttta
    34321 ttaagagtaa aacttaaata tctaaatgaa attgaaattg aaaaatcaaa aatcgtagat
    34381 agatatctta atgaaattaa taatcccaaa gtcatacttc caaagataga aaataattgt
    34441 actcatgtat ggcacttatt tgttttacaa gttgaaaaac gagatacatt tatggaatac
    34501 ttaaagaaaa atggtataga gtctataata cattatccga taccaccaca tctatctgaa
    34561 gcatatgctt atttgggata taaaaagggg agttttaata tagctgagaa ctattcagat
    34621 aaagttgtta gtataccttt atttgtcggt atggaagatg aagaagttag ttatataatt
    34681 aaaattataa atgaatatta aactaatcat aataatatca aaaataaatt ttctatatat
    34741 catttattta aatttcgtta attgacattt ttaaatattg atatattgta caaataaaat
    34801 tgaaatatat ggaagaatat agaataaaga tgagatttgt ctaatatttt tataaattaa
    34861 aaatattttc
//
LOCUS       DACTGW010000012        34518 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.012, whole genome shotgun sequence.
ACCESSION   DACTGW010000012 DACTGW010000000
VERSION     DACTGW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.012"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..2067
                     /gene="cas3"
                     /locus_tag="I9573_000496"
     CDS             <1..2067
                     /gene="cas3"
                     /locus_tag="I9573_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HAT4795130.1"
                     /translation="ILEKMNLLEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQEEIYDY
                     IEKGILEKEEFNLKDIESYINDILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQI
                     DDSVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDDMILNEIYKSQGN
                     LEKNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESF
                     YRKNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKP
                     CVIATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYL
                     DVDFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVK
                     VSFIKEKEIKSIVEGALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHS
                     RFKFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNR
                     NNKYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSV
                     VKNYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDV
                     SYKIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNST
                     VGLILK"
     gene            2508..3455
                     /gene="prmA"
                     /locus_tag="I9573_000497"
     CDS             2508..3455
                     /gene="prmA"
                     /locus_tag="I9573_000497"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAT4795131.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            3474..4232
                     /locus_tag="I9573_000498"
     CDS             3474..4232
                     /locus_tag="I9573_000498"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAT4795132.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            4234..5532
                     /gene="mtaB"
                     /locus_tag="I9573_000499"
     CDS             4234..5532
                     /gene="mtaB"
                     /locus_tag="I9573_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HAT4795133.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            5575..5925
                     /locus_tag="I9573_000500"
     CDS             5575..5925
                     /locus_tag="I9573_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HAT4795134.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            6088..6267
                     /locus_tag="I9573_000501"
     CDS             6088..6267
                     /locus_tag="I9573_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAT4795135.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            6297..6740
                     /locus_tag="I9573_000502"
     CDS             6297..6740
                     /locus_tag="I9573_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAT4795136.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            6851..7348
                     /locus_tag="I9573_000503"
     CDS             6851..7348
                     /locus_tag="I9573_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAT4795137.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            7541..8104
                     /gene="raiA"
                     /locus_tag="I9573_000504"
     CDS             7541..8104
                     /gene="raiA"
                     /locus_tag="I9573_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAT4795138.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            8317..8553
                     /locus_tag="I9573_000505"
     CDS             8317..8553
                     /locus_tag="I9573_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HAT4795139.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            8566..9909
                     /gene="yqfD"
                     /locus_tag="I9573_000506"
     CDS             8566..9909
                     /gene="yqfD"
                     /locus_tag="I9573_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HAT4795140.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            9947..10963
                     /locus_tag="I9573_000507"
     CDS             9947..10963
                     /locus_tag="I9573_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAT4795141.1"
                     /translation="MIIQKKFTIADGNFERELFGNFDENVKLIEKTLNIDVILREGNI
                     ILIGEEKNVDSALKLMNELHQTVSNGKHLDKQSISYSLSLLLEGSEQKIKELEGTIVI
                     TQRGKAVQPKTLGQKEYIKLIENNDITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRII
                     LTRPAVEAGESLGFLPGDLKDKVDPYLRPLYDALFEMLGADKFNKYLERGTIEVAPLA
                     FMRGRTLDNSFIILDEAQNTTSEQMKMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLI
                     QATEILKGVPGIGSIMLTDRDVVRHELVQRIIRAYEKHDKREEFKKEERKKVKMQEKT
                     FKRK"
     gene            11020..11481
                     /gene="ybeY"
                     /locus_tag="I9573_000508"
     CDS             11020..11481
                     /gene="ybeY"
                     /locus_tag="I9573_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAT4795142.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            11487..12206
                     /locus_tag="I9573_000509"
     CDS             11487..12206
                     /locus_tag="I9573_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAT4795143.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            12325..12726
                     /locus_tag="I9573_000510"
     CDS             12325..12726
                     /locus_tag="I9573_000510"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAT4795144.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            12790..13683
                     /locus_tag="I9573_000511"
     CDS             12790..13683
                     /locus_tag="I9573_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAT4795145.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            13695..15074
                     /gene="mgtE"
                     /locus_tag="I9573_000512"
     CDS             13695..15074
                     /gene="mgtE"
                     /locus_tag="I9573_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAT4795146.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            15147..15302
                     /locus_tag="I9573_000513"
     CDS             15147..15302
                     /locus_tag="I9573_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HAT4795147.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            15268..16041
                     /gene="recO"
                     /locus_tag="I9573_000514"
     CDS             15268..16041
                     /gene="recO"
                     /locus_tag="I9573_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAT4795148.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            16034..16633
                     /locus_tag="I9573_000515"
     CDS             16034..16633
                     /locus_tag="I9573_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HAT4795149.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            16793..17671
                     /gene="glyQ"
                     /locus_tag="I9573_000516"
     CDS             16793..17671
                     /gene="glyQ"
                     /locus_tag="I9573_000516"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAT4795150.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            17671..19737
                     /locus_tag="I9573_000517"
     CDS             17671..19737
                     /locus_tag="I9573_000517"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAT4795151.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIRLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(19903..21450)
                     /locus_tag="I9573_000518"
     CDS             complement(19903..21450)
                     /locus_tag="I9573_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HAT4795152.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            21730..22497
                     /locus_tag="I9573_000519"
     CDS             21730..22497
                     /locus_tag="I9573_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAT4795153.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            22780..22977
                     /locus_tag="I9573_000520"
     CDS             22780..22977
                     /locus_tag="I9573_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAT4795154.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            23006..24082
                     /gene="vorB"
                     /locus_tag="I9573_000521"
     CDS             23006..24082
                     /gene="vorB"
                     /locus_tag="I9573_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HAT4795155.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            24085..24804
                     /locus_tag="I9573_000522"
     CDS             24085..24804
                     /locus_tag="I9573_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HAT4795156.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            24817..25356
                     /locus_tag="I9573_000523"
     CDS             24817..25356
                     /locus_tag="I9573_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAT4795157.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            25513..26571
                     /gene="buk"
                     /locus_tag="I9573_000524"
     CDS             25513..26571
                     /gene="buk"
                     /locus_tag="I9573_000524"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HAT4795158.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            26589..27482
                     /locus_tag="I9573_000525"
     CDS             26589..27482
                     /locus_tag="I9573_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HAT4795159.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            27512..28720
                     /locus_tag="I9573_000526"
     CDS             27512..28720
                     /locus_tag="I9573_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAT4795160.1"
                     /translation="MKYAKRMDMVKASAIRASQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDIVVTENPSYLGAINAFRPYECDFLGVDTDDEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            28744..30006
                     /locus_tag="I9573_000527"
     CDS             28744..30006
                     /locus_tag="I9573_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4795161.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFSTPVLGMRWVMISLGILSIVMCPVLWFVIKDVDIREDKEEEASEKMSAGDFM
                     TVLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFG
                     APFGGYCADKVKSPTKVLLPIYVFGIAVIILFLVLPASTPMTIFIALTFVVGILGYMG
                     KGIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIF
                     QIAILIIGILSCLYILKVKKKRESKLEA"
     gene            30582..32045
                     /locus_tag="I9573_000528"
     CDS             30582..32045
                     /locus_tag="I9573_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HAT4795162.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSMKLRFFQIKDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSILFSVVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVTSSIALGIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIIANINLVPGAIMTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIIITGMYNVTDVYTTLPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKHIIN
                     VCRFMLIICAAMCCFIEPLTSWALGDICIGVLTYINLVGIFTLKKPAIKAYYDYQRQK
                     KMGVKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            32101..33237
                     /locus_tag="I9573_000529"
     CDS             32101..33237
                     /locus_tag="I9573_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAT4795163.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            33473..>34518
                     /locus_tag="I9573_000530"
     CDS             33473..>34518
                     /locus_tag="I9573_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAT4795164.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALTPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKAKKIGEKELMNLLSLKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDI"
BASE COUNT        13660 a         3637 c         6486 g        10735 t
ORIGIN      
        1 atattagaaa aaatgaactt gttagaagaa gataagttat atatatataa aagtataatg
       61 ttacatcatg gaaactatga agattatatg aggttaagta ctagtaaagt tcaagaagaa
      121 atttatgatt atattgaaaa aggcatttta gaaaaagaag agtttaatct gaaagatata
      181 gaaagttata taaatgacat tttaaatgta gattttaaat ttggtgaaga tgtattggat
      241 tatgattaca tagataaatt aagtgaaagt atgtttatag acagtgatta taacaatggt
      301 caaatagatg attcagtttt aaattataga aagtttaagt atattttata taaagggatg
      361 ctgaacttaa tagaccatag tgcatcaagt agacagaaag gtataaaatt ttataacgat
      421 tttactgatg aagaaattga tgatatgatt ttaaatgaga tatataagag tcagggtaat
      481 ttagaaaaaa ataatataga gttcaatact atacaaaaaa ggttgagaga actttctgga
      541 agaaatgttc tgactgttgc atttacaggt tcaggaaaaa cagctgcaga ttatagaaag
      601 acttttaaaa gaaaattctt tttagtgcca aataaaatat cagcagaaag tttttataga
      661 aaaaatattt ttcaaaataa aaatgattta gatacaagaa gtagtaatga ttacattgga
      721 ttacttcatg gagatataaa cttatattca gagaatgaag ataatggaga acatgatttt
      781 gtattgacat taagggatat agatttgagt ataaattttt gtaaaccttg tgtaattgca
      841 acagttgacc agttattact tagtatgttt aaatttccgg gatatgaaaa aatattcgca
      901 gccgtaaaaa atgcatctat aactgttgat gaagtacatt tattggaacc aaagatgttt
      961 ttgattttga tatattttat gcagtttgca tgcaagtact tagatgttga ttttcactta
     1021 atgacagcta cacttccaaa atcatataaa gagcaaatga taaacaaggg aattattttt
     1081 caagaagaaa gtaatgaaaa tgttactgat aaaggagaaa tagtttttat tgagagcaac
     1141 aaggaagaag aaatttgtga aggtaaagat gtaaaggttt cttttataaa ggaaaaagaa
     1201 atcaagtcaa tagttgaagg tgctttagaa aataaacaaa aaattcttat aataaagaat
     1261 actattgatt cagtcaatag aacatatgag tttttaaaag aaaatttatc tgataagtat
     1321 ggtggtgtag atatagatgt tttacatagt agatttaagt tcaaagataa aaaagagaaa
     1381 tatagtaaaa tattaaatgg agagggtgat atttggatat caactcaatc agtagaaata
     1441 tccttggatt tggatttcaa tataataata agtgatttag caactatgga tagcttaata
     1501 caaagaatgg gtagatgcaa tagaaataat aaatatgaat atggaaactt ttatattcta
     1561 cctagtgaag ataaaattta tgataacaaa cttaagaaca caactaaaaa catcttaaag
     1621 gacatattaa aaactaactc tatttttaca atggggatta gaaaaactat tttggatgat
     1681 tactatgata atagtgtagt taaaaactat tttgaagaga attttataag ttgtgataaa
     1741 gaaattaaga atatttatgg tataaataaa gaaatttttg atggattgga tttaattttt
     1801 aattttgaac cttacaaaaa tattgttgat agtaagaagg aagcagctaa aatttttaga
     1861 gatgttgatg ttagttacaa aattattctg gaagaagatt tttataagga agatagggag
     1921 ttacaacaag attcgattca agtatcaggg tttatattta atagattata ttattatgga
     1981 ttaataagca aagtagaagg atacatggta ctaaaatcta gtagtaagtt tgagtataat
     2041 agtactgtag gcttaatttt gaagtgagca tatgtttaaa tataatatac ctttagacat
     2101 tgaaaataca aaaagtacta ctgtaagccc actgtaaaaa ctatttttta aaaggaaaaa
     2161 taagttgtga gatagagtgt ctacaagtgt atatagataa taaatttgag gtttaagatt
     2221 aactaagtgg gtttcaggta catcgcgaaa ataattataa gttggagatt ttgtatgttt
     2281 aagattaact aagtggattt caaatattga tatagtagat ataatagaag ttttattatt
     2341 aggtttaaaa ttaactaatc ggatttttaa atattctgac tattatataa ggatattatt
     2401 agcttgaatt taaaaccaat aatataaaac aacaaaatat tatatttatg atataataaa
     2461 agttatggaa aaacaggaat attactatag agaagagagg taaacttatg aataattgga
     2521 tagaagtaac tataaaaaca acaacagaag cagttgaacc tataacgaat atactatatg
     2581 aacaaggagc tggaggagct gtaatagaag acccaaaaga ctttttattc caaaagaaaa
     2641 atgaactaga ctgggattat gttgaagaag aagtatttaa aaagaatgaa gaagatgatg
     2701 ttcttataaa aacttatgtt tctgaagaaa aaaatgtgat ggaatttgtc gaaataataa
     2761 agcaaaaagt tttaggatta aaagattttg gtatagatat aggtgaagga agtgtatcat
     2821 tggaccaagt aaatgaagct gattgggcaa atgcatggaa agcatactac aaaccaacaa
     2881 aagtaggaca aagggtagta gtaaagccta catgggaaga ttatgctatg caagatgggg
     2941 atttaatcat agagcttgac cctggaatgg catttggtac agggacacat gaaactacta
     3001 gtatgtgcat tagagaatta gaaaagtatg tgaataaaga ttctaaagta tttgatattg
     3061 gatgtggtag tggaatcctt gcaatagcag cagctaaatt aggagcaaaa gaagttgtag
     3121 ctgtagattt agatgaagtg gctgtaaaag ttgcaaaaga aaatgttctt gaaaataaag
     3181 ttgaaaaaag tgtatctgta atgcatggta atttaactga tgtaataaaa gataaagcgg
     3241 atgtaatagt tgcaaatata atagctgata taataaagat acttgcaaaa gatgttcaaa
     3301 actttatgaa agaagatgct atatttatat cttcaggtat aattttggat aaagtagaag
     3361 aagttaaaga aagcttaatt gaaaatggat ttgaaatagt tgaagttcaa aaattaggtg
     3421 aatggtcagc aatagtttct aagcttaaaa aataagggat atggtgacgt ataatggata
     3481 gattttttgt tgaaaaaaat aatataaatc tacaagataa aacttgcact atagaaggtg
     3541 aagatgtaaa acatatttca aaagttttaa gatgcaaact aggtgagaaa cttgaaatat
     3601 gtgacaagaa taataatgag tatatttgtg agataatgaa tatagataag agtatagtta
     3661 atttagagat tttagaaaaa gttgatataa atagagaatc agaacttaaa gtaaggttat
     3721 accagggact tccaaaagct ccaaaaatgg aaatgatact tcaaaaatta actgaagtgg
     3781 gtgtagaaga aataatatta gttcaaacta agagaagtgt tgttaaagtt gatgacaaaa
     3841 aagaagataa aaaatttgaa cgttgggaaa gaataattta tgaagcagca aagcaaagca
     3901 aaagaggtaa gataccaaaa ttaagaggtg ttttaagttt taaagaagct ttagaagata
     3961 tgaaaaaaaa taatgtaaat atttgccctt atgaaaatga aagaacagtt tcaataaaac
     4021 atgcactaaa aaaatgtgat tctaatatag atagtgttgg aatttttata ggtccagaag
     4081 gtggattttc tgaagaagaa atagagcaaa tacaaaaaaa taattgtaat gttgtatcat
     4141 tggggcctag aatattaaga actgagacag cttctgttgt agcatcaaca atagctttgt
     4201 atgaactaag tgatttagga ggagaaaaat aaattgaaaa aagtagcttt ttatacactt
     4261 gggtgtaaag ttaatcaata tgaaacagaa gctatgctag aactatttga gaaagatggt
     4321 tatgaacaag tcaatagtga agagtatgct gatgtttatg ttataaatac atgtacagtt
     4381 actcatatga gtgatagaaa atctcgtcaa tatataagac gagttaaaaa gaagaatcca
     4441 gatgcaataa tagccgttgt aggatgttat tctcaggtat ctccagaaga aattttggat
     4501 atagaagaag taaatttagt catgggtaca aatgatagaa gaaagattgt tgaagaaatt
     4561 aaaaagataa attcaagtaa gaaagttagt acagttgatg acataatgaa agttaaagct
     4621 tttgaagaaa tagaaatcag ccaaactaat ggaaagacta gagcatttat gaaaatacaa
     4681 gatggatgtg acagattttg tacttattgt atcattcctt atgcccgagg acgcgttaga
     4741 agtagagata ttgatagtat agtcgatgaa gtgaaaaaat tggctaataa tggctataaa
     4801 gaggttgtat taacaggaat acatgttgcc tcttatggaa aagatttaaa ggatagggat
     4861 attaaacttc ttgatgtaat aaaacaaata aaccagatag aaaaaataga aagaattagg
     4921 cttagttctg ttgaaccaat tttatttaca gatgaatttg taaatgaagt tttaaagatg
     4981 gataaagttt gtcctcatta ccatttatca cttcaaagtg gatgtgatga aactctaaaa
     5041 agaatgaata gacgatacac aactttagag tacaaaacta ttgtagatag attgagaagt
     5101 aaaatgccag atgtggcaat aactacagat gtaatagtag gttttcctgg agagacaaat
     5161 gaagagttta aaaaaacgta cgagttttta aaagaaatag agctttcaca aatgcatatt
     5221 tttaaatatt caccaagaaa aggaactcca gctgcaacta tggaaaatca agttgaccct
     5281 caaatgaaac actttagaag tgaacaactt ttaaatttaa gtaaagtaaa ttttaataaa
     5341 tttgcaacta aatttatcgg ccgtgagctg gatgttttgt ttgaacaaaa tatagaaggc
     5401 aataaatatg agggattaac ttcaaattat ataagagttg ttgttgaaag tgacaaaaat
     5461 atacaaggtc agatattaaa agtgaaaata aatgatgtaa aagatgaata tgtagaagga
     5521 attttgctat aacatgtaga aattataata tttataagat aggaggtgga aagaatggat
     5581 tgtatttttt gtaaaatagc gaacggagaa ataccttcaa ctaaagttta tgaagatgat
     5641 agagtcttag cttttaatga cttgaaccca gttgctccat atcatatatt agttgtacct
     5701 aaaaaacatt atgatagttt aattgatatt cctgataaag agatggacat tgtgtcacat
     5761 attcatgttg ttattaataa aatagctaaa gagaaaggtt ttgaccaaac tggattcaga
     5821 gtcataaata attgtggaag tgatggaggg caagaagtta aacatttgca ctatcatatt
     5881 ttagctggta aaaagcttcc taattacgaa gcgggacaaa actaaactaa attattgaaa
     5941 aattgagttc cttcttgata aaaatacttg ataaaagtaa aaatttagtt tataataaac
     6001 aaagtgttag gttgtaatgc atattatgca aaaagaacat agttacagtc ccagcataat
     6061 tgcgctattg gagggaggga aaaagaaatg tcagaagtaa gagttagaga gaatgaaaca
     6121 ttagacagcg ctttaagaag attcaagcgt caatgtgcaa tgtctggcat catgtcagaa
     6181 gtgagaaaaa gagagcacta tgataagcca agcgttaagc gtaagaaaaa agctgaagca
     6241 gcaagaagaa aaaacgctaa gaaatagtag taaataagaa taaagaggtg aaggggatgt
     6301 cccttaagca aaagttacaa gaggatttga aatcttcgat gaaaaacaaa gatacagtga
     6361 gaaaatctgt agtaacttta ataagagctt ctataaagca gtatgaggtt gataataggg
     6421 tcgagcttga tgaagatgga attatcgatg taatagcaaa acaattgaag caacgtagag
     6481 atgcattagt tgaatttgaa aaagctggaa gagaagactt aataaaagaa acagaaggtg
     6541 aaattgaagt tttaaaagag tacctacctc aacaattaag cgaagaagag ttagaagaaa
     6601 ttgtaaaatc tactatatcc gaagtagggg caacttctat gaaggatatg ggaaaaataa
     6661 tgtcagttat ccaaccaaaa gttaaaggaa gagctgacgg aaaacttata aataagttgg
     6721 taaaacaaaa cttacaatag attgatatag cctagagaga aatctctagg ctttttatag
     6781 tttttgggaa taacgagtca tatttgagca tatctataat caaagaaaat tttgaaagaa
     6841 ggggaaaaat ttgattatat cagtaaaaga gattaggaat tttttgataa acatatttat
     6901 accactaata ataggttact taagtagcat cttaacaatg attatcagtg gaacagatat
     6961 ttcaacctat tatttacaac tcacaaagcc aagctttgca ccaccagctt ttatctttcc
     7021 tatagtttgg actatattgt acatattgat gggtatatct tcatacctaa tattaagaaa
     7081 gggttacaac ttacctaagg taagagatgc aattttttat tatggattac aacttgtatt
     7141 aaactttatt tggagtatac tattttttgg atttggactt agatttacag ctttgataga
     7201 tataataata atgattttgg tttctataat aatgatttcg aagttttcta aaatagataa
     7261 gagatctgga tacataaatt tgatttattt aatgtggctt gtttatgcag gatttttgaa
     7321 ctattttata tggtttatta ataaatagaa tgtaaagatt ttgtaaatta ccctgtactt
     7381 ttaataatat cataacaaac gaaaaataaa tagactatta aaaataatca ttttcatata
     7441 caataatatt aatttaaata caataaattt ataaaaattg tatttaaaga ttaacatatt
     7501 aaagtattag aattggaaag gcttgaaggg agaggttttt atgcaaataa tagtatctgg
     7561 gagacaaatg aaattaactg acggaataaa aggatacgta gatggaaaat taagtaggtt
     7621 agaaaaatac cttgatcctg aatcagaagt gaaagtaaca gtaagtgcga aaaaggatag
     7681 acaaaaagtg gaggttacaa taattcctat taacggtcaa ataataagag cagaagatgt
     7741 agaagatgac ttatatgcag caattgatat tgtttgtgac aaattaagta gacaagttgt
     7801 caaatataaa actaaagtta aagacaaagt tcaaaataat aagagcataa gatttgaaaa
     7861 tcttgacttt atagataata gcagtgaatt tgatgattat gattatgatg atgaagaaga
     7921 tgaaaatata gtaatagaaa gaagaaagaa atttaatgta aaacctatga gttctgagga
     7981 ggcgatactt caaatggaac tagtaggaca caatttctat atgtttagaa accaagataa
     8041 ttttgaaata aacatagtgt ataagcgtaa agctggtgga tatggtttaa tagaacaaga
     8101 ttaataaaaa ttgtatacgt ttagatttta tataagaaaa aataaaaagg taatcatgtt
     8161 gattttttat caatatgatt acctttttat tgtttaaata atatattaga tattaggtat
     8221 ttattatata aaaattttaa aactaatctg ttacaacatc aataaagtgc ataaatacct
     8281 atttcaagca taaaattaat taagggggta atgcttatgt tagaaatatc cactgattta
     8341 caagtaaatc aaccagttat tactgttaca agtaatacgt ttattagtat agaaaattat
     8401 ctatcaatat tagaatatga agttgattta ataagaataa agactaaagt taaaacaatt
     8461 aaaatatctg gggataaact ttcacttaag tatattactg atagtgagat aggtataaaa
     8521 gggattatct acaatgttga gtatgttgat taggaggaga tacacttgat aagttttata
     8581 aggggatact atgttatagt tgtggaagga gtaggactcg aacaattttt aaatcatctt
     8641 ataagaaatg ggataagtgt ttacaatgta actagaataa aaaatactaa aatggagttt
     8701 catatagata gacaagatat aaaagaattt aagaatgttt atagaggaag taagtttgat
     8761 ataaaggtaa agcaaaaaac tggggtccct tttattatca aaagagttta taagcacaaa
     8821 ggtatgtgga tttgtgctct tgtttcttta tttttattga tgtctacatc gcagtttgtg
     8881 acagatgtat atatacaatc tcctgaaggt ataaaaaaag aagctcttag aaatgagctt
     8941 tataaggttg gagtaagacc aggagtctat aagaagagta ttgatagaaa agaagttaga
     9001 gaccatatga tgtcaaaatt taatgatgtg gcatatttat ctatcaatgt aaaaggaaca
     9061 aatatatttg ttactgttac aaaaaaggca gaatccctaa aatctacaga ccaatcaaat
     9121 tattgtaatg taattgcttt aaaaaatggt ataatagaaa aggtaatccc tagaagtggt
     9181 aaatcagtta ttaaatctgg agacatagtc caaaagggag atgtcttatt aaatggagct
     9241 aataccaagt caataccaga agtatgggca agtacttttt atgagtccac aaaaaaggca
     9301 agttatgtag atactgtaaa taaaaaaact ggagaaaaga aaaatatata tacactaagt
     9361 ttttatgata aagagtttac aatgagaaaa aatataaaat ataaagatta tgttgttgaa
     9421 aataaagaaa aaaaactatc aataggaaac tatacttttc caataaagat aaaaacgagt
     9481 actttttatg aaacaaaaaa ggttgaagta aaaaggaata aagaagagtt aaaaaaagaa
     9541 ttatctgaaa aagctttaaa agagttagaa tacataatcc cagcttcggc tagaataata
     9601 gatgtaaaac ataactttaa ggttaataaa aatatgttag aatatttaat aacagttcaa
     9661 actagtgaaa atattgctaa aatttatcct cttagtaagt cagaagcaga aaggtttata
     9721 aaggaagaaa gtaaaccaga tgaaggtgaa gaggaagttc cttccaatcc agaaaaaaga
     9781 ccattaaatg atataagaaa tgagtttgat gaggataata aggataataa agaccaaaac
     9841 aattctgatg aaaataatag taatcaaaat agtaataata gtcaaaataa taatagcaat
     9901 aacaactaat aaaattaaac ttaaaatctt aggaggacta attcaattga taatacaaaa
     9961 gaaatttaca atagcagatg gtaactttga aagagagtta tttggcaact ttgatgaaaa
    10021 tgtaaaatta atagagaaga ctttaaatat agatgtaata cttagagaag gcaatataat
    10081 tttaattgga gaagaaaaaa atgtagattc tgcattaaaa cttatgaatg aattacacca
    10141 aactgtatca aatggtaagc atttagataa gcagagtatt tcttattcat tatctctttt
    10201 gcttgaagga agtgaacaaa aaataaaaga acttgagggg acaatcgtca taactcaaag
    10261 gggaaaagct gttcaaccta aaactttagg tcaaaaagaa tatataaaac ttatagaaaa
    10321 taatgatata acttttggtg ttggacctgc tggaacagga aagacttatc ttgcggttgc
    10381 tatggcagta aaagctttta aaagagatga agttagtaga attatactta caagaccagc
    10441 tgtggaagca ggagagagtc taggtttttt accaggagat ttaaaggaca aagttgaccc
    10501 atatttaaga ccactatatg atgccttatt tgaaatgttg ggtgctgata aatttaataa
    10561 gtatttagaa agaggtacta ttgaggttgc accacttgca tttatgagag gtagaacact
    10621 tgataattca tttataatat tggatgaagc tcaaaatact acttctgaac aaatgaaaat
    10681 gtttttaact agacttggat ttggctctaa agctgtagtt acaggagatg taactcaaac
    10741 agatttacca caaaataaga agagtggtct aatacaagct acagagatcc ttaaaggtgt
    10801 tccaggtatt gggtctataa tgctaacaga tagagatgtt gtaagacatg agttggtaca
    10861 gagaataata agagcttatg agaagcacga caaaagagaa gaatttaaaa aagaagaacg
    10921 taaaaaagtg aaaatgcagg aaaagacttt caaaagaaag taaaagtgta gtaatataga
    10981 tatatagcat tattaatgaa taaaaagtag gtgtagtgaa tggatttgat actagatgat
    11041 agacaagata aattagaagt aagtgaggag ttaattgaaa agataaagga tataatcata
    11101 gaatgtttag attatgaagg atatgatgat aattatgaag tgagtctttc ttttgtagat
    11161 aataaagaga ttcatgaatt aaatagagaa tatagaggag ttgatagagt gactgatgtt
    11221 ttatcatttc ctcttttaag tgatgatttt gaggatgttg agttagaaga agaatcccta
    11281 ggtgacatag tagtatctct tgaaagagcc ttagaacaga gtatagagta taaccatagt
    11341 tttgaaagag aagtatgttt tttaatatgt catagtatgt ttcatctttt aggttatgac
    11401 cacgatacag atgaaaatac aaaagaaatg agagaaaaag aagaacacat attaaataag
    11461 ttaaacataa caagggagta gtttttatga aaccagaaaa aactcgtcaa ggtataatta
    11521 aagcatttaa tgcagctata gaagggatat tgtatacatt taaatttgaa agaaatatga
    11581 aaatacatta tttaggttca gttgctgttc ttataataag tttgtttttt aacttttcaa
    11641 aattagaaat gataatgtta cttatgtcca tatgcttagt tgtagttgcc gaaatgttta
    11701 atacagctat agaaaaagct gttgatttag taactgatga atatcatgtt cttgctaaga
    11761 tagcaaaaga tgttgcagct ggaggcgttc tagtggcagc actaaattca gtagtagttg
    11821 gatatatttt gttttatgat aaattgacag atatatctgg aatattaatt tataaaataa
    11881 gagaatcaga attacatata accttgatat gtatattatt ggttcttata gctgttgtag
    11941 ttgtaaaagc attgacttct acaggtactc ctctaaaagg aggaatgcca agtggtcatg
    12001 ctgccttagc atttgctata gcaactgcaa ttacacttat gactgaaaga gttgttgcct
    12061 ctactcttgc atatataatg gctgtattag ttgcccaaag tagaattgaa ggaaaaatac
    12121 ataccttttg ggagactatt gctggagctt tattaggtgt gttgatagct atattagtat
    12181 ttcagcttgg aatgttctat aattaaaata aatgtacata aaatatttaa aataaaacaa
    12241 atctgtgcta attaaatttt tatgatataa tgtagagtat taaataaaaa taacttttaa
    12301 ttattgtata caggaggagt tgttgtggat aacatagaat tgttaaggtt ggcagaagat
    12361 gccagacaac attcttatgc tccttactca ggttttagag taggtgcagc tcttttaact
    12421 aagagtggta aagtttatac tggatgtaat atagaatgtg catcattagg tggaacaaat
    12481 tgtgcagaaa gaacagctat ttttaaagca atatcagagg gtgataagga tatctataaa
    12541 atagctatag caagtgataa ctcagaaaat aatgagcaaa catatccatg tggaatatgc
    12601 aggcaggtaa ttatagagtt tggttctgat ataaaaataa taacgggata tactaaagga
    12661 gaggtgtttg agcatagtat aaaggatttg ttgccaaact atttctctgg aaatgattta
    12721 aaataaattt aaaagtataa ataaatttaa gtaattaaaa caagaagata aaaagttagg
    12781 agacagttaa tgttcaaatc aggttttgtc agtatagtgg gtagaccaaa tgtaggaaaa
    12841 tctactttaa tgaataatgt agttggagaa aaaatagcta taatgagtga taagcctcaa
    12901 actacaagaa atacaataca agcagtatat acagatgaag aaatgcagat agtattctta
    12961 gatacacccg gtattcacaa accaaaaaat aaattaggtg aatttatggt aaaggcagca
    13021 acagaagctt ttaaaaatgt agatttaata ttatttgtag ttgatgattc taaaaaaata
    13081 ggtcctggag atagaaagat aatagaggat ttaagaagtg ttaagacacc aataatttta
    13141 gttgtaaaca aaatagacca actagaccaa aaagatgagc tatttgatat aatcaaaatg
    13201 tatgatagag aaggtatttt taaagaaata gtaccaatat cagcattaaa aggaaagaat
    13261 acggatacct taataaaagt tatacaaaat tatttagaag aagggccaaa gtattttcca
    13321 gattatatga ttacagacca gccagaaaga gtattaatag cagaacttat aagagaaaaa
    13381 gttcttcact atcttaatga tgaaatacct catggtgtag ctgttgaaat tgaaaaaatg
    13441 aaagcgagaa atgataagga aatagtagat gtatctgctg taatatactg tgaaagagat
    13501 tcacataagg gaataataat aggaaaaaat ggtaggaaac taaaaggaat aggtaagtca
    13561 gcaagacaag atatagaatt gcttttaggt tcacaaataa atcttcaatt atgggttaaa
    13621 gtcaaagaaa attggagaaa tttacaaaat tatataaata attttggata taatgataaa
    13681 taaaggtggg ttatatggat aggaaactgg atgcttccca agatttatta tatgaagtaa
    13741 aatcattaat tgataataat aaagtattgg agttaagaga attaatagag gaatatcata
    13801 taattgatat attcgatata atggaaaatt tagaggaaga catgaaaatt caattatttg
    13861 aagtacttcc tttagatatg gcatcttcta tattagaaga aggtagtgta gagtttttta
    13921 ttagtatttt atctaaactg gatgtagaac actcgaaaaa tatacttgaa cttatgtctc
    13981 ttggagatat ggcagacaag ttgagtgaac ttgaagaaga agaaagagaa cacataataa
    14041 atcttttgaa tcaagagaat gccgattatg taaaagagtt actattctat gatgaagatt
    14101 ctgctggtgg aacaatgacc actggatata tttctataaa taaagatatg acagctcttg
    14161 aggctataga ccatatgaga gaagaagctg aagaagctga aactatttat tatatatatg
    14221 tggttgatga tgaagaaaaa ctagttggag tattgtcatt aagagaatta ataatagcta
    14281 gagacgcaaa tatagtagaa gatttgatga gtgaaaatat aatctctgtt tatgtagatg
    14341 aagatagaga agaagcagta agattagtat ctaaatacaa tttaatagca attccagtag
    14401 tagacagaca agaaaaatta aaaggaataa taacagtaga tgatataatt gatgtaatgg
    14461 aagaagaagc aactgaagat atgtataaat ttgcaggaag ttcagaacat gaaagagaag
    14521 ttgctgaaaa agaaaaccct acattgagag agcaaataat atcagcactt agaggacgat
    14581 taccttggtt aattattacc ttggttggag ggttgttggc ttcgttaata ctttctaact
    14641 tagactatat aatgaatcct gtatatgctc cattagtatt ttttatacct gtagtaattg
    14701 gtatgggtgg aaatattgga actcaatcat catctgttac agtaataaca ttatctaata
    14761 aggatttaaa ttttagcaat gtggttagag aaggtattgt aggtattatt acaggtcttt
    14821 tgtgtagtat aataacaggt attgtaattt actttgttat gagaaagtta gacattgtat
    14881 taattgtttc aatatcatta tttataaata tggttttagg tgcaacaata ggtgcgttta
    14941 tgcctgtttt attaaaaaaa atggatgcag acccatctac agtttcatca ccaattatat
    15001 ctacagctct tgatataact ggtatagcag tttactttat aataacaacg gcattattgt
    15061 caaaaattgt ttaataactt tatgtatgat ttatcctctt cttactaaaa atataagtat
    15121 aaatgtaaga agggggtttt ttaaaaatga acaatatcag ttgggaagtt tttaaaaaaa
    15181 caggtagtat agatgcatat ttatatttct gtgattgcaa aaacatacat gaagaaattg
    15241 aagaagtaag ggagaaaaaa gaagacgatg ataatcctga acacccaggg gatagtactt
    15301 aaagctataa ggtataagga aagtgacata atacttacac tatttacaag aaagcttgga
    15361 aaagtatctg caatagcaaa aggagctaaa aaaaataaga gctctttgct ttcatcatca
    15421 caattatttt cttatagcaa ttttacctta aaaaaacaag gtaatatgta caaggttact
    15481 caaagtgaaa taataaaaag tttttataat atatcctatg atatagaggc attttcttat
    15541 gctacatata ttacaaaatt agttgaaaat tctatacttg aaaatcagac taataatagg
    15601 ctatttatat tacttgcgca gactctgtat ttgtatacac aggataatac agacaatagg
    15661 tttataacag cagcattcga gttaaaattt ttagactata taggatttaa acctatagta
    15721 aataaatgca ttaattgtga atgtaaaatt ttacaaaatt ctgtgtttaa tatctacgaa
    15781 ggtggtatac tatgtagtaa gtgtagtaaa ctttttgata ataatataaa attagactta
    15841 acaactataa gtcttatgga atatgtatta agaaatgata ttttaacatg tagtaaagct
    15901 aaagtatcaa aatatataac acatgaactt gagaatattc tagacaagta tttgaaagtt
    15961 tatgttgata atattaattt taaatcatta cacgtattgc aaagtgtaaa aaataataag
    16021 ggagtggata atgatgagta gtaatataac tattgaaatg gttgattcag tctttgaaag
    16081 agttccagga gcaacttatg cagaagcaaa agaagcatta gtaatgtgca atggagatgt
    16141 aattgaggca gtaatatatt tagaaaataa aaaaaatact tgtaattgta aagcaaaatc
    16201 tgctaaagaa actatggaag aagtatttgg caaagatgga gaaaatataa agtttcaatt
    16261 aaaagagctt cttagaaaaa gtagtgtagt gagaataata gttgaaaaag atggaaaaac
    16321 tatgatgaac attcctttaa cagttggagt tgttggatta gcttttgtac cattagctac
    16381 tttagttgga ttatcagctg ctgttataag taaatatcgt ataaaagttc aaaatgaaga
    16441 agatggagaa attgtagatt taggtgaatt gaatgaagaa aaacttcacg ttttgaagaa
    16501 tatgataact aatgcagcaa aagatgtgaa ggatgtagta gttgacaaaa aagaagatga
    16561 caaagatgta actgctgacc ttatgaaaga agaatctgaa aagaataaag aagacgaaga
    16621 cgaaaataaa tagatataaa gtgactttta aaaaatgaag agaagataat tataagaatt
    16681 atcactaaca ttgacaaatt ttatagaatt tgatattcta aaagttgtag aagaactatt
    16741 aaaatagcct ttcgtgtaga cgagaggctt tattgttata ggagggattt ttatgaattt
    16801 tcaagagatg atactggcgt tgcaaaaata ctggtcaaag caaggttgta tcatgatgca
    16861 accttatgat attgaaaaag gtgcaggaac tatgaatcct aatacttttt taagatcatt
    16921 aggacctgag ccttggcaag tatgctatgt agagccttca agaagaccag cagatggaag
    16981 atatggtgaa aacccaaaca gactatatca acatcatcaa ttccaagtta tactaaaacc
    17041 ttctccagat aatatacaag aattatattt ggaaagttta aaagagattg gaattgaccc
    17101 aagtgagcat gacataagat ttgttgaaga taactgggaa gctgctacag ttggtgcttg
    17161 gggacttgga tgggaagttt ggctagatgg tatggaaata actcaattta cgtattttca
    17221 acaagtaggt aatattgaat gtgagcttga gacaggagaa attacatatg gattagaaag
    17281 attagctatg tacattcaag aagttgatag tgtttatgat ttaaaatgga atgataaaat
    17341 aacttatggt gaagtattta ataaagctga gtatgaaaac tcaatgtacg catttgaatt
    17401 atgtgatgca gatatgttgt ttaatctgtt tgatatatat gaaaaagaag cattaagact
    17461 tatggaaaat ggccttgtaa taccttcata tgactatgtg ttaaaatgct ctcatgcatt
    17521 taatacattg gacgcaagag gtgcaatagg agttagtcaa agagcttcat ttataggtag
    17581 agttagaaat atggcaaaga ctgttgcaga aacttttgta aaacaaagag aagaaatggg
    17641 attccctctt ttaaaggacg gtgacaaata atgaaaaatt atcttttatt tgaaatcggt
    17701 gttgaagaaa tgccatcaag atttgtaggc agtacactag aacaattaaa aaacaatctt
    17761 agtaaattat ttgatgaaaa tagaatagct tttgataaaa taaatgcgta tgggacacca
    17821 agaagactta ctttagtagt cgaaggattg agtgatagac aaagtgattt agaagaggaa
    17881 ataaagggtc ctgctaagaa aattgctgtt gatgcagaaa ataatttaac aaaaccagca
    17941 ttaggattta taaaaagcaa aggattaagc gaatctgaca tatattttaa acaagttgga
    18001 aaagaagaat atatatttgc gactataaag caagatggaa aagaaacttc agaagtacta
    18061 aaagacatat taccagagac tataaaatct gtagtattcc caaaagctat gcgttgggga
    18121 ggaaaaaatc ttaaatttgc aagacctata agatggatag ttgcattgtt aaatgataaa
    18181 gtattagagt ttgatttaga aggaataata tcttctaatg taacaaaagg tcatagattt
    18241 ttaggtgaaa gtacaataga agttgattct atagaagatt attttgaaaa attagaaaaa
    18301 aattatatag ttttagacca aaacaagaga aaagaaatga taagaaaaca atgtatagaa
    18361 gtagcaaatt ctttaggtgg agaagttgaa tttgatgaag atttacttga agaggtaact
    18421 tatttagttg aatatccaac tgctttttat ggagaatttg atgtggatta tataaaacta
    18481 ccaaaagaag ttgttataac tcctatgaaa caacatcaaa gatattttcc agtacttaaa
    18541 gaaggaaaac tgttacctaa ctttatagct gttagaaatg gtgattctta cagaatagac
    18601 aatgttaaag ctggaaatga aaaagtttta gaagcaagac ttgcagatgc tttatttttc
    18661 tatagagaag atactaagag aaacttagaa agctacatag aaaaattaaa aactgttgta
    18721 ttccaagtaa aactaggaac tatttacgac aaaactttaa gacttgaaaa attaagtgct
    18781 gatatacttg atagtttagg tttagtagat gaaaaggcag atactataag ggctgctaaa
    18841 ttatctaagg cagatttagt tactggaatg gtaggagaat ttgatgaatt acaaggtttt
    18901 atgggaaaag aatacgcaaa agttggagga gagaatgatg ttgtaagtga agctattttt
    18961 gaacattatc ttccaagatt tgcaggagat atacttccta agactaatgc aggggttgcc
    19021 ttatcaatag ctgataagtt ggattcaata gcaggtttct ttgctatagg aatacaacca
    19081 acaggttctc aagacccata tgctttaaga agacaagctt taggtatact tagtatactt
    19141 atggacagaa aagttgctgt ggatataaaa gtacttattg aacatgcttt agataattat
    19201 tctgaattag attttaataa agaagaagtt gttgagcaaa taatgaattt cttcaatgag
    19261 agagtaaaaa atctatttaa agatttagga ataagatatg atgttgttga tgctgtttta
    19321 agttctgata taaatgatgt ttctgatatg catatgagag ctttagagct taataattgg
    19381 ctagaaaaag atgaattagt ggagatgctt actgcattta atagagtttc tactctagct
    19441 caaaaagcag aaagtgataa aattgatgaa tctttattaa aagaagatgc tgaaattaga
    19501 ctttacaatg aatttaaaca agtaaaggta aaagttaatg aattattaaa ggataagaaa
    19561 tatggtattg ctttagatga atttgcatct ttaagaccag tagttgataa tttatttgat
    19621 acagttatgg ttatggataa tgatgaagct attaagaaca atagacttgc tttacttaaa
    19681 caagtatatg atactatgct agaaatttgt gatttatcta aaattgttta taaataaaat
    19741 ttaaactatt tataagaaat aaggctgtct caaaatagag atagtcttat ttttcattgt
    19801 tgagatgatg aaaatatatt tgatttcata gaaaaagagt gtctcataaa ctaaaaagtt
    19861 cattttgaga cacccatgct ttttgtaagt aaatataata aattatattt tatacttatc
    19921 agttattttg tttaaaatat tatcatttga atttacccat tcataaaaat tgcctgatga
    19981 tgcgtccgat attttataaa gcatttcagg aatatcacta gctttaaaat ctccaagtga
    20041 ttttcccaat ctagtcttac ctatttcaaa actgcctcca acaaataatt caaatgcatc
    20101 tgtagatata ccatcaacat tcttcttttt accacataaa cctatgctac caacttgatg
    20161 aacaccacat gaatttaaac atcctgatat atataactta ggtgctttat ttaaaatttc
    20221 ttcattattt tgtaatctaa aataatctat gatttcatgt aacattttct gactattttg
    20281 tatgcccatt tgacaaattg gaacacctat gcaagatata ctttgttcta attgagaatt
    20341 acaactaata gttttactta tttctaatat tctttttgct tcatttccat ttaaatttaa
    20401 aatatataat ccctctgtca ttcctagtct aatcatagga ttttcaacat tatctaattc
    20461 tttaagtaaa gtacttaaat cctttgttaa caaaatacct ccaactggat gtatatatac
    20521 agtgtataaa ccatcctgtt tttgaggtat tagtctagaa tcttgtatat ctattttaac
    20581 tccaggttta gagtaatcta tttcttcaat attgagttca agactaccat tttctttttc
    20641 taatttatag tattctttaa atttttctaa gaatagttct tcaccaagtg attcgaccat
    20701 atatctaacc ctagctctgt ttttattttc ataatttcca taatccataa agaatttaat
    20761 cataccttca acacaatata aggcgtcttt cggctttata agttcatcaa gttctaaagc
    20821 aactttgggt tgtttaccta gacctccacc taagaaaact ctgaaatatg gttcatcatt
    20881 tttcaaagta gcaacaaatc ctaagtcttg tatactacaa tgagcagtat ctgtgtaaca
    20941 attagaataa gaaactttta attttctagg taaatggtat gtagtagcat ttttgataaa
    21001 atatttatca gtagcaactg catatggagt aacatcaaag acttcactag ggtcaactcc
    21061 agataatgga gataatccga catttctagg ataattacca ccaccacctc tagtgaatat
    21121 atcatttttt attccttctt ccatgatatc aacaattgaa tttatagata aatcgtgtaa
    21181 ttgtatagct tgtctagttg ttaagtgtat cttatctaag ttgtgtttaa tagctatttc
    21241 atagatttta ttaagttgat actgagataa aactccagca gatactctaa gtcttatcat
    21301 aaaagatttt ttgtctcttt gagcataaac accatatcca ccagaaaatc ccttatattg
    21361 cattttattt atttctccat ttaaaaattt tgaagaatat tctttaaaat taggtagttc
    21421 atttaacaat atctttttaa aattatccat tatttcacct cattgttagt cttgtataaa
    21481 aatatatcta ttgtataata gatatatata ttattatcta gtcaggataa aaatataaca
    21541 gaataattct attaaaagta agaataataa ggacatagtg agtattttgt ttttatttta
    21601 caaattaagt ataatatttt tgttttataa aatataatta agataaatag ataattttgt
    21661 aattttaata tataatattt agtagagtgt gggagtagca cttaatattt gcaggaggag
    21721 tttttgtata tgggagaaat aataaatgca ttagatagag ccctagacat aattttgctg
    21781 ttatatcatg aaaagagaga aatgggaata actgaaataa gtaaggctat gggagtatat
    21841 aaaagtactg tacatagaac tttggttact cttgaaaaca aaggttttgt tatacaaaat
    21901 gcagagaatg gtaagtattg gctaggaata aatttatatg caataggtat ggttgtggga
    21961 gaaaaaatgt cattgactga aatagtaaaa ccatatacga aaaaattaaa tcaagaattt
    22021 aacgaagtag ttaatgtatc tatattagaa gaaagagctc aagattctcc aagaagtatc
    22081 ataatacata aagagtatgg ttcaaatcaa ttactttcag taaatccatc cgttggttca
    22141 agtagtgaat gttattgttc agcagttggt aaatgtctta tggcatttaa tgattctata
    22201 gattttgaaa aatatagaaa aacaccaata cacaagtata cagaacatac aattgataat
    22261 tgggatgata tgatgctttt tttagcaaaa ataaaagaac aaggatatgc tatagatgat
    22321 gaagaactag aacatggact aacttgtatt ggagcaccta tcttagataa aaataataaa
    22381 gctattgcag ccataagttt gtcaggtcca actataagaa tgagagaagg ggactttgag
    22441 tataagatta aaagagttat agaaactgca aaaagtatat ctgaactttt tagatgattt
    22501 ttaatctcga taataaaaat aggctatatt taaattgtta tttgtaaata tcagtttaaa
    22561 tgtagcctat ttttctttaa tctaaaatta aatattctta aaatttaaaa tgttattgaa
    22621 atatataagc gaatgtgtta tcttttaatt aagcaataaa aatagaacaa ggttctatac
    22681 tatagaacaa aattgatgca tatatagtat ttatacaaaa tataaatatt atatatttaa
    22741 aattaagtaa aaatattaca taaaggggag atttgagaaa tggaaaaagt caagttgagt
    22801 gttgaaagat gtaagggatg ctatctttgt attgaagctt gcaaaaaaga tgcaataagt
    22861 ctttctgact acacaaataa aaaaggcttt gtaactatta aagtcgatga agaaaaatgt
    22921 attcaatgtg gcgcttgtta tacaatgtgc cctgatttag tatttgagat attataaatt
    22981 atttttatta gaggggagaa aaggtatgca aagtttatta attaaaggaa atgaagcttt
    23041 agcagaaggt gctatacgtg gtggctgcag attttatgca ggttatccaa taacaccaca
    23101 atcagaaata ttagaaatga tggcatggag acaaaaagaa gttggaggag tatttatcca
    23161 aggtgaatca gaaatagcca gtgtaaatat ggttatggga gcaagtgcac ttggagcaag
    23221 agctatgact tcttcatctg gtccaggatt ttcattaaaa caagaaggta ttgcatattg
    23281 ggtatcagct ggtttgccag cagttgtagc atgtgtgcaa agatatggta taggtgatgg
    23341 atttatagga gctggtcaag ataattattg gcaagcagta aaaggcggtg gaaatggaga
    23401 ttatcattta atcgtgttag cgcctaacag tgttcaagaa aatatggata tggcatatga
    23461 atcttttgaa ttggctgaaa aatatatgca tccagtactt atactttctg atgctacaat
    23521 aggtcaaatg gtagaacctt gtatagcacc accaatgaaa gaatacgata tggataaagc
    23581 tccatgggct gcaaaaggaa cacctgtagg agaaaaaaat aaagtaataa ctgatataac
    23641 atattttgaa gaccaagata catggcaaaa agggtatata gaaaaaatga gaaaagttca
    23701 agaagatgaa caaagatggg aagagataga aatagaagat gctgaagtgg tatttgtggc
    23761 atacggtatt tcatcaagaa tagcagaagg tgcagttaga gatgctagag caaaaggaat
    23821 gaaattaggt ctaataagac caaaaacatt gtggccttac ccaagaaaag catttaagaa
    23881 attaagtgat tcattgaagg gacttgtaac agttgaaatg aacatgatgg cacagatgaa
    23941 ggaagatgta attatagcat gtgataatag atttccagta tattcagtag caacagctca
    24001 atatatggca gaagtaaatc aattaataga ttttgctcaa cagattatag atggaaaagc
    24061 taagcaagag gaggtatttt aattatggct aagaaagcac cagcaataat gatgtgtgaa
    24121 aatatgtttt gtggaggctg tggacactct atagtcgata gaattattgg agaagtatta
    24181 acagaaatgg atatatgtca agatacaatt atatgttcag atataggttg taatcatatg
    24241 tttcaattta gtatgaatgt agatgttgtc gttccacctc atggtaaaat gggtgcagca
    24301 atggcagcta tgaaaagagt tagaccagat aaatatatat ttacaatagc tggagatggt
    24361 ggagcttatg cgatagggct tggggaaact atgtcagctg ctactagaaa tgataatgtt
    24421 atattccttg ttatgaacaa taatatgttt gcaatgacag gagggcaaat ggctccaact
    24481 actatagaag gtcaatatac agcttctact ccagcgggta gaaattacgc agatcatggt
    24541 tcaacatttg atgttgtaaa agttatggga aatttagata tagcatatct agcaagagga
    24601 acaataacta accctaaaga aataaataca acaaaaagat acatcaaaaa agctctagaa
    24661 aaacaaaaag aaaagaaagg tttttgctta atagaagtgc ttgtaccatg tccaacaaac
    24721 tggggattaa agccagtaga tgctatgaag agaattgaaa atgaacttgt tccaaagtat
    24781 ccacttggag aatttattga ttagggagga aataatatgt tgaatgaaat tatatgtgct
    24841 ggttttggag gacagggagt tcttacagca ggaaaaatta taatgtatgt tgcatataag
    24901 gcagaaaaga aaattacatg gttcccatct tatggaaatg agatgagggg aggaagtgca
    24961 aattgcaatg ttgttgtaag tgatgaaaaa attgcaaatc cttgtgtaaa aaatccagat
    25021 atactattgg gaatgaacag cgtatcagta gataagtatg aaagtttaat gaaaaaaaat
    25081 ggttatatgt ttgtcaatga atcactagta agtaaggata gaaagtacag agaagacatt
    25141 aatgttataa aagtacctgt tactgaaata gcacaaaatt taggcaatga aaaagctgct
    25201 aacatttgca tgttaggtgc tatggtaaag agtttggatt tatttacaaa agaagaattt
    25261 gaaagtggta tgtgtgaata ctttgaaaat caaggcaaag gtaaattcaa ttctaaaaat
    25321 gtagaagcat ttgaagcagg atacgactac gaatgaatat tttaaatatt taaaataatt
    25381 attgaaatac ttatttttat atgttagtat ttaattaata aaatatagaa cgtcgttctg
    25441 gacaatagga cgaagtcttt aggccggaat acaagaacga cgttctgtat agtagaacta
    25501 gggggtgtta aattgaaagt tttagtaata aatcctggtg gtacatccac aaaaatttct
    25561 gttttccaag atgaaaatga aattttgaaa aaaaatataa cccatacaag agaagattta
    25621 aaaaattttt ctaaagtatt tgatgaatat gattatagaa aacaactgat agtagatatt
    25681 ttaagtagtg agaatcatag tataaattct tttgacgcag tagttggtcg tggtggactt
    25741 atgaaggcta taaaaggtgg tacctatact gtgagtgaag aaatgattga ggatatgaga
    25801 aatgaaataa atggggaaca tgcatcaaat ttaggtgcat tattagctaa aactatagct
    25861 gatgaaatag gagtacaatc atttgtagta gacccagttt ctgtagatga atttgatgat
    25921 gtatcgagga ttacaggaat atcagacata gaaaaagcaa gttggttaca tgcattaaac
    25981 cataaagctg tttcaagaaa aattgcacaa aaactagggg gaaaatacga ggattataac
    26041 tttattgtag ctcatttagg ttcaggaata tcgattgtag cacataaaca aggcaaaatg
    26101 attgatggaa gtggtggtag gtctgatggt ccattttctc cagaaaggag tggtggatta
    26161 cttgcatatc ctcttataga attgtgttac tccggaaaat atactcatga tgaaatggtc
    26221 gcaaaagtaa gttcgattgg gggaatgtac gattatttag gaacaaagga catgatagaa
    26281 attgaaaaca gaatcaagga gggtgatgaa aaggcaaaac ttataatgga tgcatttatt
    26341 tatcaagtag caaaagaaat ttctatgtat ggagcttcac tttcagggaa tgtagataga
    26401 gttatcttga caggagggat atctcactca gaaatggtag tcaaaggagt agcagaaaaa
    26461 gtttcatatc tagctccaat agagatagta ccaggagaga tggaaatgga ggcacttgct
    26521 ctaggagcac ttagagtact taatggagaa gaaatagcaa aaacttatta atatttagga
    26581 gaaagagtat gttaaaagat tttaataatt tattagaaga ggctaaaaag cttgaacctg
    26641 ttattgtaag tgttgctgtt gcagaagaca aagaagtatt aaaggctgtg aaatcagcaa
    26701 cagatttaaa cattataaag ccaattcttg taggagacaa ggataaaata gaaagtatat
    26761 caagagaaat tggattaaaa ggtcatgaaa taataggttg tcaaaatccc gatgaatcag
    26821 ttaaaaaagc tgtagagata gttaaaaata aggaagctaa agttcttatg aagggattag
    26881 taaatacaag tgtctatatg agagctatat taaataaaga aacgggtctt agaacaggaa
    26941 gattattaag tttgcttgca gtatatgaat tacctcaata tcataaactt ctttattgta
    27001 ctgatagtgg aataaatgta tcgccaaact taaatcaaaa aaaagatata atgacaaatg
    27061 cacttcttac tatgaaaagt ataggcatgg aaaatcctaa aactgcgata cttactgcaa
    27121 atgaaatggt agaccctaag atacaatcta cagtggatgc caacctactt gtagagatgg
    27181 aaaaatcggg agaaatacca aagtgtatag tggaaggtcc aatagcattt gatgtagcat
    27241 ttgatgcaca tgcagcagaa cataaaggta ttaaaagtaa tattgctggt gatgttgatt
    27301 tattagtatt tccaaatata gaagcaggga atatattagg aaagtcatgg cttagattta
    27361 ataaagctaa gtgggcaggt atagttttag gagcaacaaa tccgataatt ttaggttcac
    27421 gttctgacac agctgaaata aaaataaatt caatagcatt agcgtgccta gcatcacaat
    27481 aagaaattat tattaaggag gagaaagtat tatgaaatat gcaaaaagaa tggatatggt
    27541 aaaagcttca gctataagag cttctcaaaa gaaaatagct cagaaggtgg caagtggtgg
    27601 aactgttata tcttttgctg cagggcttcc tgacccaaat ttatttccaa tagaagagat
    27661 aagtgaaata actcaaaaag tacttaatga aaggggaaaa tttgccctag catatggacc
    27721 tactaaaggt gaagatgagt tgttagatct attagtaaaa agaatgaaag aagaagaaaa
    27781 tattgataca aaagcagaaa atattataat aactactggg tcacaacaag ggatagcatt
    27841 aagtgctatg attttactag aaaaaggaga tatagtagtt acagaaaatc caagttactt
    27901 aggtgcaata aatgcattta gaccttatga atgtgatttt ttaggagtag atactgatga
    27961 tgaaggaatg gtaacagatg atttagataa attattatct gaaaattcta acataaaggt
    28021 aatctatgta ataccaacat ttcaaaatcc tacaggaaaa acatggtctt tacaaagaag
    28081 aaaagatttt atggaagtcg tgaataagta tgatgtcata gtaatagaag ataatcctta
    28141 tggagaaata agatttacac cagagcctct acctacactt aaatctcttg atactaaaga
    28201 taaagtatta tacttaggtt cattttcaaa agtattatgt ccagggttta gagttgcttg
    28261 gatgtgtggt aatgtagaca tcatagataa ggcagaactt ttaaaacaag gtatagattt
    28321 acagtcaaat caattttcgc aagtacaagt aatagaattt ttaaagaaat acaacttaaa
    28381 tgaacatata gaaaaaataa gagcagagta taagaaaaga tgtctgctaa tgttagatgc
    28441 tatgaagaaa catttcccag aggaagctaa atatacagaa ccagatggag gaatgtttat
    28501 atgggttgaa cttcctgaaa gtataaatgt agatgattta ttggataaag ccattgatgc
    28561 tggagttgct tatgtttctg gagagtcctt ctttgcaaat aatggtccaa aaaatactat
    28621 gagacttaac tatacaacaa tgtcagaaga acaaatcgtt aaaggtgttg agatacttgc
    28681 agaagttata aaaaaagaac tcaattatgc ttgtgaataa atataaaaag tgagggttta
    28741 aatatgggag aaaaagaaaa cggtataaag tttggaaagt taatgtttca acttgttatt
    28801 atagcaatat cttgggaatt agtttatatt ataccattta tccaatatac attatatgat
    28861 ccaatcctaa aagcattaga atgtacaaac actcaactag gttttctact tacaatttat
    28921 gggttaggta atatatttgg agctcctata ggaggctggt tagcagatag atttgattat
    28981 agaaagataa ttttaggttc tgtatttctt aatggagtag tatcattttt atttgcattt
    29041 aatatgaact atccatttgc agttgctact tgggtaggtt gtgcaatcac atcattagtt
    29101 atgaactatc cttctatggt aaaaatactt agagttatag gaaaagataa tcaaggtaaa
    29161 gtttatggat tcaatgaagc tatggttgga gtttctggcg tagtaatggg agcaatattc
    29221 ttatatattt atacatgctt ttcaactcct gtattgggta tgagatgggt aatgatatct
    29281 ttaggaatat tatcaatagt gatgtgtcca gtattatggt ttgtaattaa agatgtagat
    29341 atcagagaag acaaagaaga agaagcaagt gaaaaaatgt ctgctggcga ttttatgaca
    29401 gtactaaaat caccaaacac ttggctagta ggaataagca tattctgtgt atattcattc
    29461 acagttacaa tgtcttattt tacaccatat ataacatcag tacttggagg ttctgtggca
    29521 ttgtctgggg cattagcaat aataagacaa catggtctaa aattgtttgg agcaccattt
    29581 ggaggatatt gtgcagataa agtaaaatca cctacaaaag tactactacc aatatatgta
    29641 tttggtatag cagtaataat tttattccta gtactacctg catctactcc aatgactatc
    29701 tttatagctt taacatttgt agtaggtata ttaggttaca tgggaaaagg tatatactat
    29761 gcagttcaag atgaagtaaa agttccagtt aaatattcag caactactat aggtatagct
    29821 gcagctttag gattttcacc agatgtattc caatttgcat taataggaca ctggatagat
    29881 acttatggca ataaaggtta cacatataca tttatattcc agatagcaat tctaataatt
    29941 ggtatactat cttgtttata tatattaaaa gttaagaaga aaagagaaag taagttagaa
    30001 gcataaatat tgtaaaaata ccaaattatt aagttttata gcaatactta aaatttaaag
    30061 tgcaacacac caaaatgtta tcaagttttc taggttttag tataaagtta cccctaaagg
    30121 tctggaattg agtagcaatt atcatctatt tatatattta cataaaccta tttaatttaa
    30181 attaaaagaa tttcaaaaat aggtggtaat tgtactcaat tttacacctc ataataaata
    30241 cgaaaattta aaatctaact atatactatt caattatata ttaataattg tatatggtac
    30301 tatttatata gtaacagttt caggcttttc attacatgta ttccaattta tattgtttga
    30361 ttatttgtca aatacatatc gttaaaaagt ttataaattt acgtttatat atcgattaat
    30421 attctttgtt ttagagatag atacttatag cttgtggatt aaaaataata taaatagaag
    30481 atagattttt tagatatatt gagttgaaga ttatatagaa tgttgaaaca tagctattta
    30541 aattattgtc tataaatata aaatgaatgg tggtgagtta agtgaccgat ttagttaaca
    30601 ccattaatgg aattgtttgg tcacctgtat tagttgcaat gtgtttgtgt ataggattat
    30661 atttttcaat gaaattaaga ttttttcaaa taaaagatgt aaaagaaatg tttactcttt
    30721 tgcttgaagg agatagctct catgttggaa tatcttcgtt ccaaggtttt gctacatcta
    30781 tggcaggaag gataggaact ggaaatatag ctggtgtagc tgtagcgata gcaatgggag
    30841 ggccaggagc attagtgtgg atgtgcataa tggctacatt agggtcggca actgcatttg
    30901 tagaatctac tttagctcag atatataaag atgagcatga tggacagtac aggggaggtc
    30961 ctccatatta ctttgaaaaa ggtcttggtt ggaaaaaatg ttcaatttta ttttcagtgg
    31021 ttgcaatcat aagttataca ttttttatgc ctggcacaca atcaaatact ctatatctag
    31081 caatgaatca agcatttgga ttttcaaaaa tgacaatagc agttacatct tctattgcac
    31141 taggtataat aatttttgga ggaattaaaa gaattggttt atttgctgag aaagtagtac
    31201 ctataatggg tggagcatac cttattatga cattggtaat aatcatagca aatataaatc
    31261 tagttccagg agctattatg acaatgttta aatctgcatt tggatttggt tcggtttttg
    31321 gagggatgtt aggttcagca atatcttggg gagtaaaaag aggtatatac tcaaatgaag
    31381 cgggagaagg ttctggaact tatggtgcag cagcagcaga agtgagtcac ccagccaaac
    31441 aaggattagt ccaagctttt tctgtttatg tagacacact attaatatgt atggctacag
    31501 gtattatgat tataataaca ggaatgtata atgttacaga tgtttacaca acattaccag
    31561 gtgtagaacc aggtccacaa tatgtacaga gtgcaataaa tacattacat cctcaattag
    31621 gtgatatttt tatagcaata gttttactac tatttacatt tacaacatta ttagccttct
    31681 catacatgat ggagaccaac gtatcttact tagtgactag aattaaggca gaaaaacatg
    31741 aaaagcacat aattaatgtc tgtagattta tgcttataat atgtgcagca atgtgttgct
    31801 ttatagaacc acttacaagt tgggcacttg gtgatatatg tataggtgta ttaacatata
    31861 taaacttagt tggtatattt acactaaaaa aaccagctat aaaagcatat tacgattatc
    31921 aaaggcagaa aaagatggga gtaaaaagga gcgatagaac ttttaatcct atagaattag
    31981 atataagaaa tgctgagttt tgggaaaata gatacttaaa agaggataag catgagttaa
    32041 attaaagtta taataaaata taaaaattac taaataaatt tagtgggagg taagattggt
    32101 atgaaagaaa gaataataca agtggctgat tcaaagaaag aaaaaattct agggctatgt
    32161 caatctttat atgatgaacc agaaattgct ttgcaagaat ataaatctgc taaaaaaata
    32221 tcagaatttc taagagaaga gggttttgat gttgaggaaa acttggcagg tatggctaca
    32281 gcttttaaag caaccaagaa aaatggagat ggtcctaaaa tagcatttat agcagaatat
    32341 gatgctcttc caggcaatgg acatgcatgt ggacatcatt taattgcatc tatgggagtt
    32401 ggagctggaa tagctctttc aagtatactg gatacttaca agggagaagt aagtataatt
    32461 ggaacacctg ctgaagaaac tggagatgga aagccttact tgatagaaca tggtgtattt
    32521 gatggctatg atgcagcaat gatgatacat ccaaattcaa aaacttgtgt aacacctgaa
    32581 attatagcaa taggaggtct agatttcata tttactggaa aagcatctca tgcaggagct
    32641 aagccatata acggaataaa tgctttagat gcagtagtac tactatataa taatataaat
    32701 gcactaagac aacaattagt agatggaacg agaatacatg gtattatact agaagcagga
    32761 actgctgcaa atgtaatacc tgatatggga aaagtaagat tagagataag ggcaaaagaa
    32821 caaaattact ttgatgaagt agtagaaaaa gtgaaaaatt gtgctagagg tgctgcaata
    32881 gccactggat gtgaacttga attttatcac tttgaaccaa cttgccaagg attaaatgaa
    32941 aataaagttt tggtagacat atttacaaag attatggagg aatttggaat atatgaagat
    33001 gaacaagtaa tgttaggttc aacagatatg ggaaatttaa gtcaaataat gccatgtata
    33061 catcctttga tgaagttttc tgaaaatgga gaagaactac atacaaaaga atttttggag
    33121 gcttctataa attcatatgc aaaagataga gtaattgatg gtataaaaat attagcttta
    33181 acaggattta atttatttga aaatccagag ttattgaaaa aaatgaagga agagtagaac
    33241 ttagactatt tttaattata ctctttaaaa taagaagagt gtctcaaaat gaacttttta
    33301 gtttatgaga cactcttttt tgcatgaaat taaatatatt tttattattt taacaatgaa
    33361 aaagaggctt atctgttttt atataacctt ttttaacttg tagcacaaaa tatcatgaat
    33421 gattacaatt cgaatgataa aatcgattat aagatatata ggaggggtac atatggaaac
    33481 tttagaagta agaaatattg aattgagtgg tacaaattat gagataggat acagactagg
    33541 agaattggtt gctaatatgc cagagataat agaaggtcag attaataaat ctaatctggt
    33601 aagcaaaaaa gaagaaaaag agatgataga gttgtttgat aaatattgtc caggtcttaa
    33661 tgaagaattg caaggttttg ctgatgcaat tcaagtgaat tgtaatcaaa ttttatatta
    33721 tactatgact tatcttaaac caggttgtag tcaagttgca ttaacaccag aattaactga
    33781 aaatgggcat gtactatttg cacgtaattt cgatttctca cacaatatgg aggattttgt
    33841 tttatgtaaa actaaagtaa atggaaaata tgcacatata ggtactaccg ttatgcaatt
    33901 tggaagaggt gaaggtatga atgaatgtgg attaggtgta agtcagtcat catgtggtat
    33961 tcctgtaggc aattcagatg gtttgagaaa accagcaatt gtaggtttac agttttgggc
    34021 tgttattaga tatttgttag aaaattgcaa agatgtagat gaagcattag aatatttaaa
    34081 agatatgcca attgcataca atattaattt attgcttgca gataagtctg gaaacattgc
    34141 acttgtggag actttagatg ggaaaaaaga agtaaatata atcaacagtt tagaaagtaa
    34201 gagagaatat tttttacact ctactaatca tacacatatt gataaacttc ataaattaga
    34261 accacaatca atgaaaaatt ctattcatag atataaatta attaaagaat atataaataa
    34321 ggctaaaaaa attggagaga aagagttaat gaatttattg tctttaaagt atccaaatgg
    34381 cttatcttgt aattactata atgatttttt tggaacttta aaaagtatag ttatggattt
    34441 aaatatagga aagtttaata ttttatgggg tggactagag aataaatggg agagctacta
    34501 tttaaaaaat gacattaa
//
LOCUS       DACTGW010000013        33298 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.013, whole genome shotgun sequence.
ACCESSION   DACTGW010000013 DACTGW010000000
VERSION     DACTGW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.013"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            98..973
                     /locus_tag="I9573_000531"
     CDS             98..973
                     /locus_tag="I9573_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HAT4795165.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     RGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            991..2481
                     /gene="cls"
                     /locus_tag="I9573_000532"
     CDS             991..2481
                     /gene="cls"
                     /locus_tag="I9573_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAT4795166.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            2674..2958
                     /locus_tag="I9573_000533"
     CDS             2674..2958
                     /locus_tag="I9573_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAT4795167.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            2991..4619
                     /gene="groL"
                     /locus_tag="I9573_000534"
     CDS             2991..4619
                     /gene="groL"
                     /locus_tag="I9573_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167116.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAT4795168.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVIVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            4881..5621
                     /gene="larB"
                     /locus_tag="I9573_000535"
     CDS             4881..5621
                     /gene="larB"
                     /locus_tag="I9573_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HAT4795169.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            5705..6439
                     /gene="larC"
                     /locus_tag="I9573_000536"
     CDS             5705..6439
                     /gene="larC"
                     /locus_tag="I9573_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HAT4795170.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            6469..6888
                     /locus_tag="I9573_000537"
     CDS             6469..6888
                     /locus_tag="I9573_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HAT4795171.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKVTP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      6948..7049
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7270..8805
                     /gene="guaA"
                     /locus_tag="I9573_000538"
     CDS             7270..8805
                     /gene="guaA"
                     /locus_tag="I9573_000538"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAT4795172.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTSITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            9344..10774
                     /locus_tag="I9573_000539"
     CDS             9344..10774
                     /locus_tag="I9573_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAT4795173.1"
                     /translation="MILNQIFIEDGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGEYISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKNNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(10922..11797)
                     /locus_tag="I9573_000540"
     CDS             complement(10922..11797)
                     /locus_tag="I9573_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT4795174.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETSLFDRLPRGVCLTRCGISLLDEARSILTACRNLDKKISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLLHKKLRLIELDEIDMENKMLAILHKDKNI
                     TQPLEIILDNISNVL"
     gene            11917..12480
                     /locus_tag="I9573_000541"
     CDS             11917..12480
                     /locus_tag="I9573_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HAT4795175.1"
                     /translation="MNQSRKIYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCTAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSTMIPIAFFANFVM
                     SINVIFILSVCCFVCVMQVFYKKRENK"
     gene            12477..13043
                     /locus_tag="I9573_000542"
     CDS             12477..13043
                     /locus_tag="I9573_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HAT4795176.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     ILLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(13172..13408)
                     /locus_tag="I9573_000543"
     CDS             complement(13172..13408)
                     /locus_tag="I9573_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795177.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(13718..17146)
                     /gene="uvrA"
                     /locus_tag="I9573_000544"
     CDS             complement(13718..17146)
                     /gene="uvrA"
                     /locus_tag="I9573_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAT4795178.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFENELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFAKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKTPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPNKNYTALTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFTETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCNEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            17465..19699
                     /locus_tag="I9573_000545"
     CDS             17465..19699
                     /locus_tag="I9573_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT4795179.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMDEILNIDSFNGEGTIEIINSQGKTLFIGKNNELIKDLSYKYKDSADEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDVVLGELNK
                     LNSFTIIMTFITTCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSNFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            19955..21985
                     /locus_tag="I9573_000546"
     CDS             19955..21985
                     /locus_tag="I9573_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAT4795180.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDLIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENEIILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            21982..22434
                     /locus_tag="I9573_000547"
     CDS             21982..22434
                     /locus_tag="I9573_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT4795181.1"
                     /translation="MNKLVNVKCSFKIHANSKEEAIVSLVDVVSKEGYLIDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDIPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            22436..23491
                     /locus_tag="I9573_000548"
     CDS             22436..23491
                     /locus_tag="I9573_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAT4795182.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            23558..23866
                     /locus_tag="I9573_000549"
     CDS             23558..23866
                     /locus_tag="I9573_000549"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAT4795183.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            23970..25235
                     /locus_tag="I9573_000550"
     CDS             23970..25235
                     /locus_tag="I9573_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphate kinase"
                     /protein_id="HAT4795184.1"
                     /translation="MKKLPIKTVVQRLLDLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLSVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            25323..26039
                     /locus_tag="I9573_000551"
     CDS             25323..26039
                     /locus_tag="I9573_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAT4795185.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(26234..26527)
                     /locus_tag="I9573_000552"
     CDS             complement(26234..26527)
                     /locus_tag="I9573_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012423205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HAT4795186.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(26533..26748)
                     /locus_tag="I9573_000553"
     CDS             complement(26533..26748)
                     /locus_tag="I9573_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795187.1"
                     /translation="MRTLSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            complement(27293..27805)
                     /locus_tag="I9573_000554"
     CDS             complement(27293..27805)
                     /locus_tag="I9573_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAT4795188.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVKQILDLPVKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            28133..28801
                     /locus_tag="I9573_000555"
     CDS             28133..28801
                     /locus_tag="I9573_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4795189.1"
                     /translation="MEFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNI
                     GKYKERDIYINECIEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEI
                     LANQKKITVNNYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIE
                     NSYGNNKISNMDKIFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKALQDEEFFIFKI
                     KIFS"
     gene            29254..29367
                     /locus_tag="I9573_000556"
     CDS             29254..29367
                     /locus_tag="I9573_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HAT4795190.1"
                     /translation="MPRCRSPPFLSEKQADKKEMEETLWQKSIIFISEDKR"
     gene            29325..29735
                     /locus_tag="I9573_000557"
     CDS             29325..29735
                     /locus_tag="I9573_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAT4795191.1"
                     /translation="MAKEYYLYIRGQKVKVSEDIYIVYWREKEHEKYLEQVDRKNHLL
                     FFSALDHDGHFTDNIVDESVDVEKIVETQMMIEAVRSAITRLNAEERDIIERLYFNDE
                     TVRSVAKLKSITHPALIKRRNKILEKLKKFIEEL"
     gene            30477..32153
                     /locus_tag="I9573_000558"
     CDS             30477..32153
                     /locus_tag="I9573_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAT4795192.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIDGYSLEAQKSRMKAFALYNDYEIV
                     GEYEDAGKSGKSIEGRVQFTRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDFGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRVQTMEGRIQKAKEGKWNG
                     GFAPYGYQLIDGKLIINEEEAIAIRTIFDQYVNTSIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYRQVEQDDYLVAEGIHEAI
                     IPDELWQAAQVKLKAQAKKYEHVNKGKNMRTHLLSGIVKCPICGAGMFGNKSIKYKKD
                     GTKYKDFYYYGCKHRLMNRGHKCTYNKQIREELLDDAVAEVIIKLVSNPKFASMIQEK
                     INMKVDTSAIENEIDNYQNELRKSHSTKYKLIEEIDNLDVEDKHYKRRKNDLDDRLYR
                     MYDKIEELESQLIEAKAKKETIEVEKLTGDNIYKVLIYFDKLYKVMNDVERRQLIEAL
                     ISEIQIYEEKQPNGQWLKSITFKLPIIDEDLNISLENDEQVETVAKIIKQ"
     gene            complement(32631..33269)
                     /locus_tag="I9573_000559"
     CDS             complement(32631..33269)
                     /locus_tag="I9573_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795193.1"
                     /translation="MSNQIKDFDITNDMFQESIPITTKTILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLVKAANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
BASE COUNT        12584 a         3753 c         5711 g        11250 t
ORIGIN      
        1 ttatctttgt gttaaaataa gtaaaataca catattaaag ttaagttttt aggcaaataa
       61 acaaaatgaa aatataaata ttaaggagat ttggtatatg aatgtttatg aaaaaggtaa
      121 tggagttata ctagaaggag taacagattt tgacccagta catatatttg agtgtggtca
      181 atgttttaga tggcacaaac aagaagatgg ctcatacact ggagttgcaa gggggagaat
      241 attaaatgtc aaaaaagaga atgataaaat atatttaaat aatacgaatt taaaggagtt
      301 taacagcatt tggtataatt actttgattt aggaactgat tatacagaaa taaagaataa
      361 gctaaaaaat atggatgaat atttgaataa ggctactgaa tttggatggg gaataagaat
      421 attaagacaa gatggatggg aaatgcttat atcatttata atttcctcta ataatagaat
      481 tcctatgata caaagagcga tagaaaattt atcaagaaag tttggtaagt atataggtga
      541 atatgaaggt aatgaatact atgcattccc aacaccagaa gaactaaata aggcctcaca
      601 agaggaaata agagcatgtc aaacaggttt tagagataag tacataaaaa gtactacaca
      661 ggcagttata gagaataatg atgaggtatc tgaatataca aatttaagta cagaggattg
      721 tagaaaagag cttttaaaat ttaatggagt aggaccaaaa gtatgtgatt gtattgcatt
      781 atttggtatg caaaaatatg attcttttcc agttgatgtt tgggttaaga gagttatgca
      841 agaattttat atagatgaag acatgagttt acctaaaatg agaacttatg gaatcgataa
      901 atttaaagaa atgtctggat ttgctcaaca gtacttattt tactatgcaa gagaactcgg
      961 tataggaaaa taaattaagg gggagaaatt atgggagtaa taggaacaat atttctgttc
     1021 tatttaatta tatcatatct agctggagcc ataatttctg tgattatatt gcttgaaaat
     1081 agagaccctg caaaaacaat gtcttggcta ttgatgttta ttatatttcc aggtgtaggt
     1141 ttaatgatat atgctatttc aggtagaaat attagaaaaa gaaaattatt taagactcaa
     1201 aaactagcta ataatataaa agaaaagaaa ctatttgata cattagaaaa gataacagaa
     1261 atagttgaat tagaaaaaga atctataaaa caaaataagc ttttaagaga tgaagaggat
     1321 ggtagttata gaaaaagggt aataaatatg ttgttaaaga ctggtatgtt tccctttact
     1381 aaaaataata aagtggatgt atttgtagat ggaaatgaaa agtttaaaag acttatagaa
     1441 gatataagag aagctaaaga ccatattcat ttagagtact ttatcatcaa agatagtgaa
     1501 ataggtaggg tgttaaaaga agaacttata aaaaaggcta aagaaggcat taagataaga
     1561 attttatatg atgatgtagg atgctggaga ttttggttta atagaaaatt tttccgagaa
     1621 atgagggaag taggtataga aattgcagca tttttaccta caaaatttcc tataatagga
     1681 gggaaattaa actatagaaa tcacagaaaa attgttgtta ttgatggaat aattggatat
     1741 acaggtggaa taaatatagg agatgaatat ttaggcaaaa atgataagtt tggatattgg
     1801 agagatactc atattaggat taagggtatt tctgtataca tgcttcaaat gacatttctg
     1861 attgattggt attatacgac aaaagaagta ttagtgacta aaaattattt cccaagtgta
     1921 gggaatgttg gtgaaagtat gatacaagtt gttgcgagtg gtccagacag tgactgggaa
     1981 gatattcatt atgcttattt ttctgcaata tgtcaggcta gaaaaaatgt ttatatagaa
     2041 actccatatt ttattccaga tgaaagtcta ctaaaagcta taaagagtgc tgctcttagt
     2101 ggagttgatg tgagaataat atttcctaaa attgcagacc acaagatagt aaatatagca
     2161 tcttattcat attttgaaga aatattaaga gcaggtggta aagtttattt atataataaa
     2221 ggatttatac attctaaagt cgtaataata gatgataaaa ttgcttctgc aggtacagct
     2281 aatatggatt taagaagttt tatgcttaac tttgaggtaa atgcatttat ctatgatgaa
     2341 gaagttatta gggtaatgac agatgatttc tttgaggatt taagtcactg tgaagaactt
     2401 aatttggagg tatttaagaa tagaaatatc atacaaaaaa tcaaagaatc tgtagcaaga
     2461 ttattttcac caatattata aaaaattatt aaaaaaggta ttgaaaatta tgtgtgattg
     2521 gtatattata ataattgtta gcactctatt agatagagtg ataataaata aaaagtatat
     2581 atataaatct acatatataa gtagtattaa tttaaaaata actaaaaaca taggcgaact
     2641 agtctatact taatatttta ggaggcgtga ataatgaaaa taagaccatt agctgacaga
     2701 gtagtaatta aaaaagtaga agcagaagaa aaaactgcaa gtggaatagt tttaccagga
     2761 gcagctaaag agcaacctca aatagctgaa gttgtagaag ttggaccagg tggaatagtt
     2821 gaagggaaag aaataaaaat ggaattgaca gtgggagata aggttatatt ccaaaaatat
     2881 tctggaacag aagttaagat agaaggacaa gaatacacaa tactaagaca gagtgatgta
     2941 ttagctgtta ttgaataaat atagaataaa tttattagga gggatttaaa atggctaaag
     3001 aaattaaatt ttcagaggaa acaagaagag ctttagaagc tggtgtaaat aaattagcag
     3061 atacagtaaa agtaacatta ggacctaaag gaagaaatgt tatattagat aaaaaatttg
     3121 gttctccact tataactaat gatggagtaa ctatagcaaa agagatagaa ctagaagata
     3181 gatttgaaaa tatgggtgct caattagtta aagaagtagc aactaaaact aatgatgtag
     3241 ctggagatgg tactacaact gctacagttt tagcacaagc tataataaga gaaggtttaa
     3301 agaatgtaac agcaggggct aacccaatac ttttaagaaa aggaatacaa aaagcagtaa
     3361 tagtagcggt agaagaatta aaaaatcaat caagaatagt agaaacacaa gaggctatat
     3421 ctcaagttgc ttctatatct gctggtgatg aagaagttgg aaaattaata gcagaagcta
     3481 tggaaatagt aggtaaagat ggagttataa ctgttgaaga gtctcaaact atgaatactg
     3541 aattagatgc tgttgaaggt atgcagtttg atagaggatt tgtttctgca tatatggtta
     3601 cagatgtaga taaaatggaa gcagttttaa atgacccata tatattaatt actgataaga
     3661 aaatatctaa catacaagaa ttattaccag ttcttgaaca aatagttcaa caaggtaaaa
     3721 agttattgat aatagctgaa gatgtagaag gtgaagcatt atctacatta gtagtaaata
     3781 aattaagagg aacatttgat gtagtagcag ttaaagctcc aggctttgga gatagaagaa
     3841 aagaaatgct tcaagatata gcaatactta caggtgctca agtaatatca gaagaattag
     3901 gttatgattt aaaagaagct gatttatcta tgctaggtag agcttcatct gttaaggtaa
     3961 ctaaagaaag tactacaata gtagatggtt ctggagataa aaaagctata gaagatagag
     4021 taactcaaat taaacatcaa gtagagcaaa ctacttcaga ttttgataga gagaaattaa
     4081 tggaaagatt agctaaactt gctggaggag tagctgttgt aaaagttgga gctgctacag
     4141 aagtagagtt aaaagaaaga aaattaagaa tagaagatgc tcttaatgca actagagcag
     4201 ctgtagaaga aggaatagta gctggtggag gaactgcttt tgttagtgtt ataccagcaa
     4261 taggaacact aattgagagt ttagaaggag aagttaaact aggtgctcaa atagttaaaa
     4321 aagcgttaga agagccatta agacaaatag ctataaatgc tggtcttgaa ggtgctgtaa
     4381 ttgtgcaaaa tgttgttaat tctgaagcag aaactggatt tgacgcattg aatgaaaaat
     4441 atgtaaatat gatagaagct ggtatagttg acccaactaa agttagtaga agtgctttac
     4501 aaaatgctgc atcaatagca agtactttct taactactga agctgctgtt gctgaccttc
     4561 ctgaaaaaga agatgcagga atgccaggaa tgggtggcgg aatgcctggt atgatgtaat
     4621 ttaaaaaatt taaaatataa attttaatat aaagaactat ttcgacttag attttaaact
     4681 ctaagttgaa atagttcttt tgtatatatc taatttttgt atatctataa attcaataca
     4741 catatttaga taatcagtat ctagtttgta ataatatttt agagtagtat tgttattttt
     4801 taggttatga ttttagatat atattaatta aggtataatt gttgatagag aataaatcaa
     4861 atttaaaagg atgcgataat atggacttaa gaaaattact tgaagaagtt aaaaatgaaa
     4921 atattgatat agattttgcg ttagaaaagt taaaagattt accttatgaa gaccttggat
     4981 atgcaaatat agaccatcat agagaattaa gaaatgggta tccggaagtt atttattgtg
     5041 aaggtaagag tgatgagcat attttaggga ttattgataa aatgaatcaa aaaagttcta
     5101 acatattggg gacaaggtgc agaaaagaaa cttttctgaa aattaaagaa atctataatc
     5161 atgctgagta cgaagaggcg tctaaaattc ttaagattca aaatcatgat atagagaata
     5221 taggaaaagg taaaattgtg attgctacag ggggaacttc tgatatacca gtagcagacg
     5281 aagcttatca tacagctaag tttttaggta atgatgttga tagaatatat gatgttggag
     5341 tggcaggtat acataggctt ttaaataaaa gacataaaat agatagtgct agagtaatag
     5401 tagccgttgc aggtatggaa ggtgctcttg caagtgtaat tggtggactg gttgatgttc
     5461 cagtaatagc tgttccaact tctgttggat atggagcaaa ttttggagga ttagcagcat
     5521 tactagctat gttaaatagc tgtgcatctg gaatatctgt tgtaaatatt gataatggat
     5581 ttggagcagg atatttagca tcaatgataa ataaactgta aatacattga tataaattaa
     5641 acaaaactat ataataaaat ttaatttcta ttatattttt actattaaaa ttgaggagac
     5701 atatatgagt gaaaaaatac tttattttga tataataagc ggtatatcag gggatatgac
     5761 ccttgcaagt ttattaaatt taggtgtgcc taaggaaatt tttttggagg aaattcataa
     5821 actaagaatg agcgatgaat ttaatataaa catcagctcg aaaactgaaa atggaatagt
     5881 aggtacaaaa gtagaagtaa ttacaaaaga aaaacataca catagaaatt tagtggatat
     5941 atttgaggtt atagataaaa gcactctaaa cgaaaatgta aaatctaaag caaaaaaaat
     6001 atttatggta attggagaag cagaagcaaa agttcatgga actacaatag acaagataca
     6061 ttttcatgag gtaggggcta tagattcaat tgtagatata gtaggagctt ctatattagt
     6121 tgacttactt tgtgtagata aagtttgtgc tagtacagtg tctgttggtt caggatttgt
     6181 aaaatgtgaa catggaatta tacctatacc tgcacctgca actatagaaa ttttaaaagg
     6241 tgtgccaatt aaattaaata atgtaaatgg agaatgtaca actccaacag gagctgcaat
     6301 cataaaagtc ttgtgtgatg agtttgtaga tgagcttgaa ttttctccaa agcaaattgg
     6361 atatggtata ggtcataaga agtttgaagt accaaatata ctcagaactg tattaggtga
     6421 aaaaaaaaag aagaattaat ttatgaaatt agtgcaaaca ttgatgatat gtcttcagaa
     6481 atctattctt atttgtatga aaaaatatta gatgagaaag cattggatat ttatacagag
     6541 agtatttata tgaagaagaa tagaccagca acaaaagtat caattttatg taatgaatca
     6601 gatttagaaa agtttattaa aatattactt ttagaaacta gtacttttgg agtgagatat
     6661 aatgcatata atagagaaat tttagagaga aaatttgtaa aattagatac aaaatatgga
     6721 gtggttacag taaaactagg gtattataaa ggaaaactag tcaaggttac tccagaatat
     6781 aatgaatgta aaactatagc aaaaaatgaa gatattccta taaaaaaagt gtttgctgat
     6841 ataaatttta gaataaaaga agaatttagt gaaaacttat tgacataagt aataaatttt
     6901 gataaattaa tattcagcct tattaaataa aagtaaacag aaaaaaatat ataaaatata
     6961 aactcactcg tatatgctca gaaatatggt ctgagagtct ctaccaagat accgtaaata
     7021 tcttgactat gagtgaaatt attataccaa aagacctagt taagtaaatg tgtaggctga
     7081 gttatactcc ctatgtgttg gctataagat aaaggtattt tggaagcctg tatagtaatt
     7141 tcacttttgc tagaaattat ggaagttact atacaggttt tttgtgtaaa aagatttaat
     7201 ataatttaga ctgtattgtg ttaaaataaa tatataaaaa ctatattata caaggagaga
     7261 tgtgaattta tgaaacatga attagtactt gtaatagatt ttggaggaca atataatcag
     7321 ttgatagcta gaagagttag agaaaacaac gtatattgtg aaatacttcc atgtactgca
     7381 agcatagaaa gaataaaaga aaaaaatcct aaagggataa tatttacagg cggtccaaat
     7441 agtgcgtatt tagaagattc tccaacaata tcaaaagaaa tatttgaact aggagtacca
     7501 atacttggaa tatgctatgg tattcaaata atgtctcatg tattgggtgg agttgtaaga
     7561 aaaggtaata aacaagaaaa ggaatatgga aaaacatcta taacttatgg aaaatcatca
     7621 ttatttgaag gaataactac aaatagtgta tggatgagtc atacagattt aatagaaaaa
     7681 gttccagaag gatttactat agttgcaaat actaatgatt gtccagtagc agctatggaa
     7741 aatgtagaac gaaacctata tggagttcaa ttccatccag aggtagaaca ctgtttggaa
     7801 ggggacaaga tacttacaaa cttcttatat aatatttgta aagtaaaagg tgactggact
     7861 acagattcat tcatagaaga taaaataaaa gaattaaaag aaaaaatagg cgataaaaaa
     7921 gctttatgtg ctttaagtgg aggagttgat tcttctgttg ctgcagttct tattcataaa
     7981 gcaattgggg acaacttaac ttgtatattt gttgaccatg gtttacttag aaaaaatgaa
     8041 ggaaatgatg tagaaagaat atttagagag aaatttgata taaatttaat aagagttaat
     8101 gcagaagata gattcttatc taaattaaaa ggagtttccg aaccagaagc taagagaaaa
     8161 ataataggtg aagaatttat aagagtattt gaagaagaat caaataaatt aggaaaaatg
     8221 gatttcttag tacaaggaac aatctatcca gatgttatag aaagtggaca tggaaatgct
     8281 gctactataa agtctcatca taatgttggt ggaatcccag aagatgtaga cttccaagaa
     8341 attgtagaac cattaagaga gttatttaag gatgaagtta gaaaaatagg attagagctt
     8401 ggtatagaag aaggattaat atttagacat cctttcccag gaccaggtct aggaataaga
     8461 gttataggtg atgtaactaa agaaaaatgt gatatattaa gagaagctga tgctgtttat
     8521 atggacgagc ttagaaaagc aggtttatac agagaaatat ggcaagcttt tgcaacatta
     8581 ccagatgtta agactgttgg agtcatgggt gatgaaagaa catatgctta tttagttggt
     8641 ttaagagcag ttacttcttc tgatggaatg acttctgact ggtataagat gccttatgat
     8701 gtattagaaa gaatttctaa tagaataata aatgaagttg atggagtaaa tagagtagtt
     8761 tatgatataa cttctaaacc tcctggaact attgaatggg aataaaatat agaatgccag
     8821 aaatccacat gaatacagga tttgaggtct ctcttacttc tatattatac tggtttgata
     8881 ctatcaatat ttgccagtat cttatggatt aataagcaaa aaacatcctg ctgaatggta
     8941 aattcggcgg ggtgttttta ttttgttttg aaactccttt gctctgaaac tcgaagtatt
     9001 taacactgat gatgttgtta tcttttagtt ccttttgtgt catgctctga aagacaaatt
     9061 gatacaataa aaaagtaatt tcttaatcat tgatggagat atgtatttat gttactcttg
     9121 atggtaattt gagtaatatt gttcatagct ttttatattc acttaattag aaaaccattt
     9181 ggtataatca taaataaact tgatgatata atagaaaaga tttataatta ttagaagtat
     9241 ataaaagtat tttatgtcta tgaaatgtat gtctataaaa catatagatg acttttgtat
     9301 attaatgtat gaatattgat attatagaag aattgagttg tcaatgatac taaatcaaat
     9361 atttatagaa gatggaggga ttatgagaaa taaaataatt ataggaatta ctttttcact
     9421 tgtattacta atgtctggat gtgcttcaaa acaaacagca gaaataaacg tattagcaac
     9481 tactgattta catggagtta taccttatga gttaacttct tatgtaaaag aagaaagaaa
     9541 aaaagataaa aatctaactt tagtagatgc aggagatttt tttgatagtg gagaagtgtt
     9601 tggttctagc atggatagtt attttagtgc tagatataaa gatggtgata ataacactga
     9661 aagttatatt gagacgccta tttcaaaaga tatgaaagag gtaggttatg atgttgcagt
     9721 tcttgggaat catgaattta tatccaacaa taaattttat ctagataata tgatatctga
     9781 ttttgagaaa caaaaaataa atattttatc ggcaaatacg tataaaaaaa atggagatag
     9841 ttatacaaaa ccatacataa taaaaaatat taatacacct gaaggaaacg taaaactagg
     9901 aattttaggg cttactataa aagaagttgg tgagaggaaa cagtggaaag atggaaaact
     9961 tgttgactct aagtctttag aactaaaaga ccaagagggc tacaatggtg aattgtatat
    10021 gaatgattta gtagaagatg ctaaaaagtg ggttggtata atggaaaaag agaagcctga
    10081 cattatagtt gctgtggcac atacaggtga gaaacctaag aaacctagaa acccaggaaa
    10141 tagaatacaa gacttagctc aacaagttga tggaattgat gctatagttg caggtcataa
    10201 tcatgttcaa attgagcaac atgattataa aaataaatca ggagaaaatg ttatagttac
    10261 tgagccagga aagcatgggg aatatatttc taagattaat tttaaattag aaaaaaacaa
    10321 tgataaatgg aatatagttg acaaatctag taaattaatt aaatttgaaa aaaatcaaga
    10381 aagtgataaa tttggagaat ttatttcttc aattaataaa gtaagtaaag ataagaaaga
    10441 aataagatta tcagaagtaa agactcctga atgggataaa atatatgttt ttaaatctgg
    10501 aacatctgca gatgatatat ataaaacaat aggatataaa tggagaaata tatcatctcc
    10561 aaagggtgat gacatggtgc agattgttgt aatgaataat aataagcctg ttagctatat
    10621 atatggtgaa gctgaagtta tgccaattaa tataaaatgt gatgaatcag agtataaaaa
    10681 taatgttgta actatttatc ctaataaaaa tgataaattt aaaattgaaa aaggtaagca
    10741 agaatatagt attaatttaa aacatataca atagaaactt ttatcttatt taagtttaga
    10801 ttgtacttaa aataaagtgt gtattatttt aaaaagtgat gataattaaa atcacaacat
    10861 taaggaatag tacacacttt ttatgctaaa aaatagaatg ttaaaaaatt aaacctttaa
    10921 attaaagtac attgcttata ttatccaata ttatttccaa aggttgtgta atatttttat
    10981 ccttatgtaa tattgctaac attttatttt ccatatctat ctcatctaat tcaatcaatc
    11041 gcaatttttt atgtaagagt gaatctaata ataaattttc tggcaaaatg gttataccta
    11101 accctgcttc tgcagcttta ataagtgcaa aagaattaac actttcccaa acaggataag
    11161 ctttttggtt tgaaagagat aatacactgt ccaaagtatc gcgaatagca cttccttgtt
    11221 cacgtaacaa taacggaaac gtgcataact gtcgtagaga taaggtttgt tcagataaag
    11281 gaaaagttgg agcacatgca acacataact tataggaacc taataaaatt gtacggaatg
    11341 ccccctgtgg ttctatacct tcccaaaaac atatatcagc ttctccactt tttaaaatat
    11401 ccatagctga attagcactt tcgacacgta cattcacttt aagagatgga aatgaatttt
    11461 ttagtcgact taaaattggt ggaagtaaaa aagaggcaat tgtaatgcta gaaacaatgt
    11521 ttataggtgt acattcttct aaacgactga tttttttgtc taaattgcga caagcagtta
    11581 agatactacg tgcttcatct aaaagagaaa tgccacagcg agtaagacat acccctcgtg
    11641 gtaatctgtc aaatagtgat gtttcagctt gttgttcaag ttcagctata gcatgagata
    11701 cagcagattg tgtgatatgt aatttttttg cagcacctgt aaaagtgcct gtttgttcaa
    11761 ttgcttctaa aatctctaaa tatcgtatgt ttatcataat aaatatcctc actatacatg
    11821 aataaaatta attaattcca ttaaattcta tcattttaca gattgcaaag caacaggtat
    11881 actgaataat atgaagtgaa aagaaggagg aatattttga atcaaagcag aaaaatatat
    11941 ttttggcttt taattattaa cttgtttatt agcacattca cttttggcgg agggtatgta
    12001 gttgtaccta tgattagacg ttattttgta aatcaaaagc aattctttac agaagatgat
    12061 ttgatgagta tggcagcagt tgcacaatca actcctggag ctattgcaat taatttatct
    12121 gctttggcag gttatcgagt agctggtact ttaggggttg tcattagttg tacagcagct
    12181 ataatacctc cactcgttat tcttggatta gtatcagcat tttacaccac ttttatatct
    12241 aatgccattg taactgcagt attaaaagga atgcaagctg gtgttgctgc actaattgtg
    12301 gatttgatta ttgacatgtg ctctatgata ttaaaacaac gttctttgtt tttatcaact
    12361 atgataccta ttgctttctt tgcaaacttt gtgatgagta taaatgtaat ttttatatta
    12421 tctgtttgct gttttgtatg tgtaatgcaa gttttctata agaagaggga aaataaatga
    12481 atacaatttg gaacttattt tttatatttt taaaaatagg cttgtttagt attggtggtg
    12541 gttatgcaat cataccttta atccaagaga aagtagtaaa tcaaactgga tggataagtg
    12601 agaaaatgtt tacagatatt ataactattt cacaaatgac accagggcca ctggctgtta
    12661 atacttctac ttttgttgga cttcaaattg gagggttttg gggagcagtt gcagccactt
    12721 taggttgtgt attatgtggt gtagtaatat ctctattttt atataatttt tttcaaaagc
    12781 atcagaactc tacttatata tttgaaatat taaatggtct aaaatcagct tcattaggtt
    12841 taattatctc agctgcggct acaattataa tccttgcatt atttgggacg aataaagtgt
    12901 acttcaattc tatatttgag atgctaaact ggactgcact aataatcttt tttgttatac
    12961 tactgatagt acgtaagtgg aaaattaatc ctattgtgat tataataata agtggtatac
    13021 taggaatatt tttttattta taagtttttg gtaagtaaag gatatctatt tttttatatc
    13081 ttaaatccaa atagccaata aagtaaggct atttggattt ttaataaatt ctgaaaagat
    13141 aatcagaaac ccatataaca tttaaattat atcactggtt ttctacaaac caatcataag
    13201 cttcttggca aaatttctca ctatctatat agtaactatt atgaacatta ttaatttcaa
    13261 caagtttctt tttacatttt agtgaattat aatagctatc aaaatattgg gttttaaaat
    13321 atctatcatg aagtgtttta aagacaagag taggtatgtt gactccatct tttatcttac
    13381 tatcttctgc aataaagcat tctaacattg tattaattat ctttgtacta tttttccaaa
    13441 tatgaggatt tttcttttca aatattttat aaaattcatt atcatagact tcattataac
    13501 ctttatattt aggtggtttt actgttatgt gaaaattagg aagaaactta aagaaaaaat
    13561 ataataattt ttatgttctg ttttatatat tattcatatt ttatcacaga taatatacta
    13621 aagttaattt ttttattgta caactattat aataattctt ctataatata agacattcta
    13681 taattttata tttaagttca tgtgtattta attgttttta tataaaatgt ccagtaatac
    13741 tatttggatt tatttttatt tcatcaggag ttccagaagc aacaatttcc cctccattta
    13801 ttccactgtc tggacctata tcgataatcc aatcagaagc acgtataacc tctgtattgt
    13861 gttcaataat taaaagtgaa tgctgttcat ttacaagttt tctaaaagta atcaacaaat
    13921 tttctatatc tttaaaatga agtccagtag taggctcatc caatatgtac agcattcgtt
    13981 ttttagtttt tgtccctaat tcttttgcta attttatcct ttgagactct cctccgctta
    14041 atcctaatgt attttgtcca agctttagat atccaagtcc cacttcatct aataaggaga
    14101 gttttctata tatatcagag caatcactaa aaaatgtttt tgcttctgct acttccatgt
    14161 caagtatatc tgcaattgtt tttcccttgt attgtataga cagtatttct ttttgatatc
    14221 tcttaccttg acattcatta caagttaccc attggtctgc catatattgt agaataattt
    14281 ttgttttacc taatccttga cagtttgggc attgcccctt tttgctatta aagctaaaat
    14341 agctttcatc caataatttt tgttttgctt gtggagtttc agtaaataga gaacgaattt
    14401 catcaaaaat attgatatat gtagcaggtg tagaacggct agattttcca ataggtgttt
    14461 ggtccattaa gataaaatcg tcaatagcat caaatccagt aagtgcagta taatttttat
    14521 ttgggataga tttttgtttc aatttttctt ctagagcagg aagtaaactg tgaaatacaa
    14581 gagaactctt accagaacca ctaacacctg taatagagca catacagttt aagggaattt
    14641 ctacatcaat attttttaag ttatttgcat ggcatccctt taagcttaac catttacttg
    14701 gtataatttt attttgtgaa cttatcttat tgtatgtact caaatatttg cctgttatag
    14761 aatttggatt ttttataatt tcttgggttg taccttctgc aatgatgaag cccccttttg
    14821 taccagcatt tggtcctaca tcaataatat aatcagcaga cagtattgta tccttatcat
    14881 gttccactac tataacagta ttacccttgt ctcttaattc ttctatcatt ttgatgatta
    14941 gattatggtc tcttggatgt aaaccaatag atggttcgtc taaaatatag gttaatccta
    15001 ctaattcact tcctagctga cttgataatc gtactctctg cagttctcct ccagataaag
    15061 ttggagctgt tctatctgtt gatatatagc taagacctac ctttaataga taactaactc
    15121 gaatttttaa ttctgttaag atatcattta ccaaagatag ctcatttttt tgaagttgat
    15181 ttggtaattc acacaaccaa ttccataact gttctatcgt catttttgtt agttcaggga
    15241 agcgataacc tttgattgta gtgaatctag cctcaatgca cagcaattcg ccaccacatg
    15301 tattgcaagg aattttgttc atatattgat gcaaggtatt attttcagaa ctagattctc
    15361 taaaaagtct ttgaatgtgg ttgattgctc ctgaagcagg ccttctaatc tttgattctc
    15421 gtccctttga ttcaaagcta aattcatata tcttatcgtc tgtaccatat aagattgcat
    15481 ctataaaatc tcttggcaat tcattccaag gtatatctag gtctatattt ttatcttttg
    15541 ctattgcata taattcacct accatccaat taccatttgg ctttttcccc ctcaattttc
    15601 caaaatagta agtagctcca tctaatattg aaatagttgg gtcttttaca attaattgag
    15661 gattaacttg ataggtaaaa cccaatccat tacaatcgtg acaagctcca gtatgggagt
    15721 tagcattgaa ggttgaagta tttatcttag aaaaaatagt ttggcaatgt ggacaataat
    15781 tttttgtatt attctttttt tctaatggag ttttgcaata tggacagtaa ctttttccga
    15841 ttgatacaaa taacattctt aagtaatccc aaatgcttgt aagagtacca acagtagaac
    15901 gaggattttg gcttcctttt ttctgttcga tagctatagt aggggttaat ccctgaagta
    15961 aatcaaattc cggtttttga agctgttctg aaagttttgc tccattacct agattttcaa
    16021 aataacgtct ttttccttct tcataaatcg tatcgaaagc taaagaagat tttccactac
    16081 cacttacacc tgtaataaca gttatttgat tttttggaat tgtaacctca acatccttta
    16141 aattgttttg ccttcctcct tttagataga tagagctttt attatgacaa agtgaatttt
    16201 taattgattt atttattgct tttgcaaatt tgaatttctt ttctagttca agaggattgc
    16261 ctaattcttt tatttccgaa atacctaata tttttaactg agaaaagatt gatttcaaat
    16321 aatcttcagg caatttttct attaacaaat taaggtcttc tatttttata tttccgcttg
    16381 ataatatctc tgccatttca tctaataaat ttttttctgc tcttgttaat tgattgtatt
    16441 ttttggaact taattctgtc cataaatccc atggaagcaa ttctaaacca atcatattat
    16501 taaagtcatg tagaagatta ccctttacag aatgccaacc tttccaattt ttattatatc
    16561 caaagtcatc tggatgagca tgtccttgtc gacatcttat ccaggcttct gaaccagtta
    16621 aaaaaccatc tgtttttccg acatcatgag tgtcaaaatt aatttgaaaa taattttttt
    16681 gtatgtcatc tatttgtgca tctattctta tccatctctt tgttagagta tcatagtatt
    16741 ctacaagcca gtggtcttca taagtaagct cattttcaaa ataattagca aaacctgctc
    16801 tcattctagc ctcaaaacct cgatatctta ggagcgatac aagaagtaaa gaataatctc
    16861 tgcacattcc aacaagcctt tgttttgggt ttcttggaat agttagtggg ttagaattta
    16921 tttcaaatat tctttttaac atttgaggaa ttgtacgaag caattcctca tcagattgtt
    16981 gcttattaaa ggatatacca tataattttc cttggtctcc atgaattact aggctttgaa
    17041 ccacttttaa taactcaatt ggagtctttg gtaaatcttg atatttattt tcaaattttt
    17101 taggatttgt gatgatgctt tgatttttat agttatttat tttcatttat atttcacctc
    17161 tctaaatgga tgatataccc tgacatggtg tcatatgcaa ggacaattta gattttttgg
    17221 ccaaaattta aaaataaata aaaaatgata cagtgataaa ttgtaataca aatatagttt
    17281 caaattgcat ataatataat tatttaataa aaccattatc tatggaatgt taatgaaatg
    17341 ttaagatggc attcttgaat attaagaaat agaatactat aatatagtgt gaaggattct
    17401 tagtaaaaaa atttgtaagt gttaatatac attaaaaaat aggtttataa tttggaggaa
    17461 aaaaatgaaa ttatcaaggt ataagaaaaa tagtttagtt ctgtctataa ttttatgttc
    17521 acttattatt tcaatccttg taattactgt ttttaatacc gtaaaactac acaatatatc
    17581 taataaacaa tctaaaaact atttaaacga tgtatctaca cagattgtaa tgaatgttga
    17641 ttctaaaatc aaatttattt tatccgactt aagaataatg gcagatttta ttaagcaata
    17701 tgaaggtgat agtcgatatg agtatttatc taaaagaaag actagttata agtattatga
    17761 tgttggaata atagatttag aaggtaaagc tgagttttta agtggaaaaa agtttgactt
    17821 aaagaatact gcttcctata aaaaaggaat tgaaggtaaa gaatactcag aagtaataga
    17881 gtcaatgggt tttgttcttt atagtgtacc tatatttaac gataatcatg aagtgtctag
    17941 tattttaata ggtgtatcca ataaagaaag catggatgag attcttaata tagatagctt
    18001 taatggagaa ggaactattg aaattattaa tagtcaggga aaaactcttt tcataggaaa
    18061 aaataatgag ttaataaaag accttagcta taagtataag gattctgcag atgaaccttg
    18121 ggcacaaaag atgcttaatg attttaaaaa tgataaatca ggaaatatta caataacctc
    18181 atcaaaaggt gtacaatgct tattaactta tcatccaatt acaagaggtt taaatgattg
    18241 gcattttctt ctcatagtac ctgaagatgt tgtacttggt gaacttaata agttaaattc
    18301 ttttacaata ataatgacat ttataactac atgtattatt ggaattataa cattaatttt
    18361 atatattata agaagaaaat atgtaaatca aatagaaaat attgcatatt tagatagtat
    18421 aacaaatggt ataaattcaa ctaagtttag tatgctagtc aaacctttaa tttcagaatc
    18481 acctgatagt acttatatga tgatagcaat gaatataaaa gattttaagc taataaatga
    18541 ttgtttcggt agtgaaaaag gaaatatgac actaaaacat ctttacaata ttttgtataa
    18601 aaacattgat gaaaatgaag aatttgtgtg ccgtcatgat gcagaccttt tctatttgct
    18661 tattaagaat agaccagtgt tagaagccct ggagttatta tataaaatag aagctgacac
    18721 aaattatttt aataaaaata gggaaaatcc atacttttta agactatcag taggtattta
    18781 cacaattgaa aatcataatg aagatttaat tacaatacaa gaccatgcaa atacagcaag
    18841 aaaaagcttt aataaaactc atcaaagtga tttttctttt tatagtgata tagaaagaaa
    18901 acgattaata tctgaaaaag aaatttcaaa tttaatggaa aaagcccttg aaaataaaga
    18961 attttttatg tgcttacaac ctaaaattga tataaaaagt ggaaaaatta gtggagcaga
    19021 gtctcttgtg cgttggaaaa attcagaaaa gggcataatt tatccaagta attttattcc
    19081 tcttttcgaa aaaagtggat ttatatgtaa gttagattta tatatactag aagaaacatg
    19141 caaattgatt tctaagtgga taaaagaagg taaagaagtg ttgagaattt ctgttaatgt
    19201 atcacgtcaa catttgaatg ataaattgtt tttagaaaaa tataagcata tatgcaataa
    19261 atacaatgta ccaacatgtc tgatagattt agaacttacc gaatctattt tcttagaaaa
    19321 tccagaagct atagatataa tagaggatat tcattctaat ggatttaaat gttctataga
    19381 cgattttgga tttgggtact catcattagg gatattaaag gattttaaag ttgatataat
    19441 taagttagac cgttcattct ttgttagcaa aaataatata gaccgtggta aggtagttat
    19501 taagtctatt attgaactta gcaaaaggct aggtatgaaa gtaactgctg aaggaattga
    19561 agaattagaa caagttgagt tcctaaaaaa tgtaggatgt gattatattc aaggttatgt
    19621 tttttctaaa ccattattaa tttctgattt tgaaaatttt gcatatgaaa ataataaaat
    19681 taaaaatatc cattattaaa tgtacaagat aaaaaattta ggcaagttat ataattaaaa
    19741 atataacttg tcttttttat ttatatattt aataaaaata tatttgcaga ttttgtaaaa
    19801 ataattttca aatgttgtaa gttgtggcta aagctttatt gtattattct gaatatagaa
    19861 agatagttac tgtcttagat aaaaactgaa acatacataa taatataaaa tttgctatat
    19921 taataactct aattattttg gaaggaggga aaatatgata agtgggagaa tgttactcat
    19981 catgaatttt ctaaaaggaa aaaataagac aagttacaag gatattagca aacaacttag
    20041 tatagaagaa agaaaagtta gatatgatat aaataattta aatattgttt tgcgttcatt
    20101 aaacaagtca gtaatacaaa agatgaacaa aggtgtgtta attattcctg atgacttcca
    20161 agtagtatgt ataaataata acgaatatgt attttcctta actgagcgag tatctattat
    20221 gaaaattata tcaatgttta aaatagataa tctaaactta gaaaaattga gtaaagaatt
    20281 tcaggtttct agaagttcaa ttaaaaatga ccttaatgtt ttgtcaagtg aattagaaag
    20341 aaaccaaata actattactt atgataaatc ttttaaaata agtggagaag aagaccttat
    20401 atataagcaa agattgaaca tattaaaatc gtattcttat ctttttggta aggaaaaatc
    20461 tgatttaagt gtatttgaga agtatttagt gaatgtaatt gaaaaaatat tcaaagggat
    20521 ttatttgatt gatatatata attggtctat gaatttatta ttgcaaatgg gatggacttt
    20581 aaatgatgaa tcttttacat ggtatgtgtc aaacattttc ctttttacat ggtatataca
    20641 ttcagatgaa aaacatcctt tagaatctgc ttcattaagt gagccatgtt ttgaaaataa
    20701 gtttgaccaa gaatttgaaa caattattca aaaaaagttg actaaggaac aaatttttct
    20761 gttaaagagt tttgtatttt ttacaaataa atatgcaagt ttaaacgagg atatggattt
    20821 gattagtaca gaaattatag ttcaagacct aatttacaat atgtcagaaa tattagagat
    20881 tagctttaaa gaagatatga ttctttataa aggattactc aatcatattg ccccattgat
    20941 agagagaatc aagcagaata tacagattta tgaggaatta cttcatgtaa taccagataa
    21001 atatcaatat gtacttgaag ctactaaggt ttctattcaa aaaaacagag ttttaagtca
    21061 aataaagaat gaaaatgaaa taatactttt ggcaatacat ttcttaggaa gtatacatag
    21121 aaatgagagg aataactaca aaaatatatt gttagtttgt ggatttggat atggtgtaat
    21181 tgctatgtta aaggatagtt tagtaaatga ttaccagata aatatagtag agagtattcc
    21241 atcatataag ttacaagatt ataaagaatg ggaaagtatt gacttcatta taacaacatc
    21301 aaagatagta atagacttgc ctaaaacaat tattgagata aatccatttt tacaagaaga
    21361 ggattatttg aagtttgaag aaaaagggat aaaaagaaag aacgtcttaa ctaattatat
    21421 gtcaataaaa aaaaggttgg actttttaga agaagatgct caaaaaagag tattaagtat
    21481 tatatgtcaa gaacttggat acagtgatga gagaatatta tttagacaac ttaatttaag
    21541 tgatttaata gggattgata ccataaggat aattgatgaa gatatgaaat gggaagatgc
    21601 tgtaaaaatt agttcaaaca tacttgctaa ttgtgatttt gtatcagcac aatatgctga
    21661 gaatattata gatattttaa aaaatgtggg tttttatgct gtaaaagata acgaatttgc
    21721 tttactacat ggcaatgata gttcattagt caaagtaagt tcgataagtt tattaatatg
    21781 taaagaagca gttagttttg gagataaaaa ggttaaaatc atattttgtc tagcaagcag
    21841 agataaaaaa gagcaaattc ctgcaataat taatctaact aaaatggttt ataagactaa
    21901 ttttattgct atgttagaaa gtgcaaagac aaaagaagaa gcagaagttt taattcataa
    21961 atatgagaag gaggtcactg tatgaataaa ttagttaatg taaaatgttc atttaaaata
    22021 catgctaatt caaaagaaga agctattgta tcgttagttg atgtagtatc aaaagaagga
    22081 tatctaatag ataaaaatca atttttggaa gatgttttaa agagagaaga aacattgtca
    22141 acttatattg gacatggtat aggtcttcct catagtcaaa gtgtaggtgt taaaaactct
    22201 tgtattacaa taggaaagct tgatattcct attgaatgga cagaagaagg agaaaaagtg
    22261 gatttaatat ttttaatatc agtaacaaaa gacaatgaaa ataatcttca tttaaagatt
    22321 ttatcaaaat tagcacgttt attgatgcat gaaagtttta gaaatcaaat tagatgttca
    22381 gatgaacaaa ctgtttataa tcttataaaa gaaaaaatag aggaggaaga ttaaaatgag
    22441 aattttaaag tttttgcaaa aacatcttat gacagcaaca tcttatatga ttccatttgt
    22501 ggttgcagga ggaatattat ttgctttatc agtaactcta agtggacaag ctgctgttcc
    22561 tgaaacaggc tggttggcta aactgaatca aattggagct gctggcttgg ctttattcat
    22621 tcctatatta ggtggatata tagcatttag tatggcagat aaaccaggat tagctccagg
    22681 tatgataggt gcatatttag caaaagaagt aaatgcaggt tttataggtg gtatcatagc
    22741 aggatttata gcaggatttg ttgttttaca attaaagaaa attaaattag ctcctacaat
    22801 gagaacttta ggttctatat ttatttatcc tttgattggt acattgatta caggtggtat
    22861 aattgtattt ttaattggag aacctatagc ttcttttatg acatggatga caaactggtt
    22921 aaatggaatg tctggtgttt ctaaaattcc tttaggtgga atcttaggag gaatgatagc
    22981 ctcagatatg ggtggtccta taaataaggt tgctgctact tttgcccaaa ctcaagttga
    23041 cacacttcca tatttaatgg gtggagttgg cgttgcaatt tgtatacctc caattggttt
    23101 aggattagct acattattgt ttccaaaaaa atttagcaag gaagaaagag attcaggtaa
    23161 agcttcgcta ttaatgggat gtgttggaat tacagaagga gctattccat ttgcaacatc
    23221 agatccagtt agagtaatac cttgtattat ggctgggtcg attataggaa atattatggc
    23281 ttttttatta ggatgtttaa atcatgcacc ctggggaggt ctgatagtat tacctgtagt
    23341 tgacaatagg ttggggtata ttgcatcagt attaactgga gcagtagttg ttgcagtatt
    23401 aatgaaatta gtaaaaaaag acgtgaaaga agatgaagaa attgaagagg atttagatga
    23461 atcaattgaa ttaatatttg aagaattata aatataaatg gaaggaattt gtataaaaaa
    23521 gtaaatataa aactataaac ttaaaggaga taagaatatg aaaattgtag cagtaacatc
    23581 ttgtcctagt ggagtagcac atacttacat gtcggctgaa tcacttgaat tatcggccaa
    23641 aaaatttggt atagaaataa aggttgaaac tcaaggaagc tctggaatag ataatgaatt
    23701 gtcacttaaa gatattgatg aagcaacttg tgtaatattg actaatgatg ttgagattag
    23761 aaatatggaa cgttttaagg gaaagaaggt agtaagaatg agtgtatctg agataattaa
    23821 gaaatctgat gcattaatta agaaaataaa agatactttt caataaaaat tgatttattt
    23881 tacatataac tcaaagatgt ttttaaagaa atttttatta aaagcatctt tgaaaatatc
    23941 ttaaaaatag aataaaagga gatatagtca tgaaaaaatt accaataaag actgtcgtac
    24001 aaagattact agacttacaa gaagagggga aaagtgcaac tcttttagga attggtccta
    24061 tgtcaccaaa cttgttacaa gcaagttttg aactagcaaa agatgatgat tttcctttaa
    24121 tgtttatcgc aagtagaaat caagtagatg cagatgaact aggtggcggt tatgtaaatg
    24181 gttggaatca ggaaactttt acaaaagata ttaagaagat tgccgacaaa gttggatttg
    24241 atggtttata ttatttatgt agagaccatg gtgggccatg gcaaagagat aaagagagaa
    24301 atgaccattt gtctgttgat gaagcaatgg agttaggaaa aaaatcttat ttagcggaca
    24361 ttgaagcagg ttttgaccta ttgatgattg accctacaaa agacccattt gaaattggaa
    24421 aagtaattcc actagatgta gtactagaaa gaactgtaga attaattgag tattgtgaaa
    24481 acgagagaaa aagacttaat ctaccagaca taggttatga ggttggtaca gaggaaacca
    24541 atggaggtct tacttcaact gagacgtatg aaacatttat aactagacta aaggttgaac
    24601 ttgataatag gggtttgcca atgccaacat ttatagttgg tcaaactggt actttaacta
    24661 gaaagacaga acaagtagga acttttaatt ttagaaatgc ttatgattta gctcaaatgg
    24721 ctaagaaata tggagttggt ttaaaagagc ataatggcga ttacttagat gatgtaactt
    24781 tattagaaca tataccatct caaattattg ctacaaatgt agctcctcaa tatggtactg
    24841 aagaaactag ggcatattta aaattagcag aagttgagtg taaattagaa aaagagggcc
    24901 tagttgataa aacttctaat atcagacatg ttcttcttgt taatgcaatt gaatgtggac
    24961 gttggagaaa atgggtagta ggagaacaaa aaaatctaac tactgaagag atatttaaga
    25021 atgaagtttt atctaatgaa atactagata ttgcaggaca ttatacattt aataatgatg
    25081 atgttaaaaa agaaatagaa gtgctttatg ataatttaag taagaataat attgatggtc
    25141 aaagatttgt tgtagaccat attaaacgtc ctttgcgtga ttatgcagaa tgctataact
    25201 taaaaggtgt tactacgagg attttaaata aataatagga cttatagtgg tgagtattgt
    25261 tatactaggt atttatagtt ataattatac ttaccactat taaatttagg aggactaata
    25321 ttttgattaa tttaattttt gatgtagatg atactttata taaccaatta acgccttttt
    25381 acactgcata taataaagtg ttttcttcaa taaaggatat ttctattgaa gatttatata
    25441 tgagtagtag aaagtatagt gatgaggtat ttcatatgac tgaaaatgga gaaatgccaa
    25501 tcaaagaaat gcatatttat aggataatga aagcttttga ggagttggga aattctatta
    25561 cagaaaaaga tgcacaaagt tttcaagatg agtatatata tcagcaatca caaattacac
    25621 taataccaga agttgagagg attttaaatt tttctaaaga aagaaatatt aatctaggaa
    25681 ttataactaa tggcccatca aaccatcaaa gaatgaagtt aaagcagtta aatattgaaa
    25741 attgggttga taaaagtaat atttttattt ctagtgaggt tggattttct aagccagata
    25801 ctaatatttt tagagtagct gagaatgtta tgaatttaga tagagaaaat acatattatg
    25861 tgggtgattc ttatagaaat gatgtattag gtgctaaaaa agctggttgg aagagtatat
    25921 ggttgaacca tagaggacat gaggtagaag agttgtttta taaacctgat tttgtaattt
    25981 tggaacataa ggatttgata tctttattta taaaaatatg tagttataaa aatatgtagt
    26041 aaaaaaatgt taataataga attgtataaa ataattaata ataactatat aaaattatat
    26101 gtttagattg tgagcaatct attttatgtt aaaaatagat tatttagaag aaggggaaat
    26161 cttaaaaaaa tttgaatatc ccctttttat tatttaatta aaatgaaata gttagaaata
    26221 tattttattt agtttagtca ataacatttt gggattcttt ataactacat gttaactgtt
    26281 gaataggctt atcatatggt ttaacccatc ctttattaat acttaattct gtaagtgctg
    26341 tgtgaccttc taccatttga gttagtgaag ctgaatatat agctcttaat tcaggtgtac
    26401 tacttgttaa tgcagaatta agatacatat tggcagatgc tttagcagat gaaagcatac
    26461 ttaacattat tactttatcg tctatataag tattgttctt aattagattt tctatcatat
    26521 ttctcatgtt atctatacct cctcagaaat tagtatttca ttttcaacta taaattgttg
    26581 tatagcttta attcttcctt ctgttgctaa tatactagat tctgattgtc tttttaggtc
    26641 ttcatctgat attaacatat ttattgctct ttgcataagt aatccgtcag actccatttt
    26701 tataactgct gctaaagata ttttttcagt ttctgaaagt gttctcattt aatcatctcc
    26761 tatattgtaa tttatgcttt aatagtatgt ctttcttttc aaggaaataa ccattacact
    26821 tttttacagt attttatatt ctctttatat caaagtttag tcaacttaat atattaatat
    26881 aattttgtag gatatttatc attaaatatc tataaaataa attatttgaa tattcataaa
    26941 tcaaaattta tttgtatgta cttcttcaag agtcaatatg aattttttca aaaaattaat
    27001 tttgatgata atcattaagg gctatatttt tcctaaagtt gtgattatca ttattaattt
    27061 actaaaatac tttaatgaaa aatagctaac tttaattgta ggatattctt aatatatttg
    27121 aaataaataa aaatattgaa ggatataata ttcaattggt atataaataa taaatgatag
    27181 ttaagattta ccttagaggc taattaggtg ttcagtcaaa attaatgcct ttagaataca
    27241 aatgtattct aaaggcattt ttactatgta ttttcattta tattactaaa cattatctat
    27301 ttttatatac atttctatgt attttatttt catcaaatcg gtctataaat tcattttttt
    27361 cttcactata tccaatagat ataagtgcat aaggaactaa ttttactggt aagtctaaaa
    27421 tttgtttaac tttatttact actttatttt taggaaatac tcctgcccaa caagcagcta
    27481 gaccttgatt agtaacttct aataataaat tttgtgtgca agcacctaaa tcttgtatcc
    27541 atttaccagg ttttaagaat ttatctctat ttccaagcac tactatacat aatggtgcaa
    27601 attctatatg ctttgctcta tgctgtgact ttgagagctc tttcagcagt tccttatcat
    27661 ctactataat aaattcccaa ggttgagcat tttttgatga aggtgcttgc atagctgatt
    27721 ttagtatttc agttatttta tcttcttcta tatttttatc tatatatgtt cttattgaac
    27781 gtctattttt tataacttct aacatacaga ttctccttta aataaaatta tttctattta
    27841 tataatatca ttactttaag ttttagctat tacttatata aagaataaac tttgtgttgg
    27901 ttttatctaa gtaaaagtaa cattcagaac taaaaaacaa gactataaat ccaagatatt
    27961 aattaaaaaa gaagtaataa aaaataatat acaagaaata aaaattgtta ctgataaaat
    28021 gatgaaactt gataaaaaaa cgcgtttaaa agttagctct aatgatgaag ttggagaatt
    28081 aaaacaacaa attaatgatt tgtattctac tttattaaga acaattgacg atttggaatt
    28141 taaaaataaa gaaattttaa aattagaaaa gttaaaatat gactttttta aaggtgcatc
    28201 ccatgaactt aaaactcctc ttgctagtct taaaataata ctggaaaaca tgaaatacaa
    28261 tattggaaaa tataaagaaa gagatattta tattaatgaa tgtattgaaa tcgttgatag
    28321 tttaacaaaa aatatttctc aaatattatc agttcattct atagaaaatt tgaataatga
    28381 tgaagaatat ttgaaaataa atgatacatt agaagacata ttaaaaaagt atgaaatatt
    28441 agcaaatcaa aagaaaataa ctgtcaataa ttatatatta gatgagaatg tttatatagg
    28501 aaaaacagct ttaaagatta ttctatctaa tttgattagt aatgcggtaa aatatactga
    28561 tgtaaatggg gtaattaata ttgggatagt taatgattgg ttatatatag agaattcata
    28621 cggtaacaat aaaatttcga atatggataa aatatttgat gttaaatttg atttaaataa
    28681 ggaaaatagt aatggattag gtttatatat tgtaagtaat attctgaata attacaatat
    28741 agaatataaa gcattgcagg atgaagaatt ttttatcttt aaaattaaaa tattttctta
    28801 aacaagatat aattttgcaa tagttattga aaaatagaga cttattatta gccatggaag
    28861 taaaggcttt tttcttattg aaagtgaaca gaaaataatt gatacaagaa atcaggattt
    28921 gaacacgcta atgaaaatat tgttagtaaa actatatgag ttagtctttt cggatgcaga
    28981 gactgtttaa atatttgata gaatagaaac ttaaaaagtt taataaaact caattatata
    29041 agtagtagat gaatatgatg gttatttaga ttattgataa aatggaggaa ataaatatgg
    29101 aagcatctaa acgtttaatg ttagaaatat ttagaattca atacattcct cttttttata
    29161 atatgtatag tagatgattg tttcaggtgc gaattgaaac aatcaagttc atataaagaa
    29221 atatttttat tctttcattt cagaggactt cttatgccaa gatgcagaag tcctcctttt
    29281 ttatctgaaa aacaagcaga taaaaaagag atggaggaaa ctttatggca aaagagtatt
    29341 atctttatat cagaggacaa aaggtaaaag tcagtgaaga tatatatata gtctactggc
    29401 gagaaaaaga acacgagaag tatttagagc aggtggacag gaaaaaccac ttgctctttt
    29461 tttcagcatt ggatcatgac ggacacttta cagataatat tgttgatgaa agtgttgatg
    29521 tagaaaagat tgttgaaaca cagatgatga ttgaagcagt aagaagtgct ataacaaggc
    29581 tcaatgcaga agaaagagat attattgaac gcttgtattt caatgatgaa actgttcgtt
    29641 cagtagcaaa gctcaaaagt attacacatc cggctttaat caaaagaaga aacaaaattc
    29701 ttgaaaagct gaaaaaattt atcgaagaac tttaaaactt tggtaaccaa agggggcaaa
    29761 ttttccttat gtaaagtgaa ggggagtttg acctccttta ctttcttgag taggagatac
    29821 gcctgcttat ggcaaatagg aaaatgtaaa gaaaacagtc cctttcaaat attgatcttt
    29881 gacaactgaa tagaccaagg taggacttta tctacttact gagagttatg acctacgcca
    29941 agacttttct gctgatgaaa atcggttcaa atgaataccg gcataatgcc gaggttagaa
    30001 gaaaatgagc gatagatagg aatacataaa agataagcgt ggcagcttat ttcgatgatg
    30061 taagtggtga taatgatact tcaatagttt aagctgcctc gtctgaaaca aggggcggag
    30121 gtgagattcc tatgtagcag ccaatgcacc taccaatgtc ataaaactta gtaagaaaaa
    30181 taatatttgg ttataaagaa tttaaagtgg aggaggtgta ttagtggata atgactggaa
    30241 tggattggct gatttgatag caaatctgat tacaaaatat gctggagttt tagatttaga
    30301 gaatcttcca aatcctactc cagtaaaaaa tacagaggat aagaataaat ttgatatagc
    30361 taaaagcacg gattgagaaa gagaaataaa agtgctataa tatacttgag attaaagtcc
    30421 aaacctttat tggtaaaaat atataaatat ataataagga tattgaggtt aatgctatgt
    30481 caaaagaaaa aataaaagta tatctctata caagagtatc tacatccata cagatagatg
    30541 gttattcttt agaggcacaa aaatcaagaa tgaaggcttt tgctctctat aatgattatg
    30601 agattgttgg tgaatacgaa gatgcaggta agtctggaaa atctatagaa ggtagagttc
    30661 agtttactcg tatgatggaa gatataaaat ccggaaagga tggagtgtct ttcgttcttg
    30721 tatttaaact atcgagattt gcaagaaatg ctgctgatgt actttcgact ttacaaataa
    30781 tgcaggattt tggagtaaat cttatttgcg ttgaagatgg aattgattca tctaaagatg
    30841 cgggtaaatt gatgatttct gttttatcgg cagtggctga aatagaaaga gaaaacattc
    30901 gtgtccaaac aatggaaggt cgcattcaaa aggcaaaaga gggtaaatgg aatggtggat
    30961 ttgctccgta tggttatcaa cttattgatg ggaaattgat aataaatgag gaagaagcaa
    31021 ttgcaataag aactattttt gatcaatatg taaatacaag tattggagct aatggtcttt
    31081 ctaaatattt agaaaatcat ggtataagga aaataccaag acaaaatggg aaaaatccat
    31141 tgtttgacgc aggtcttata agaaaaatat taaagaatcc tgtgtataac gggaaaatag
    31201 catttggaag aagaacttta gaaaaagttc atggtacaag gaatgaatac agacaagtag
    31261 agcaggacga ttatttagta gcagaaggca ttcatgaagc aataatccca gatgaattgt
    31321 ggcaggctgc acaagttaaa ctgaaagcac aagctaaaaa gtatgaacat gtaaataagg
    31381 gcaagaacat gaggacacac ttattatcag gaatagtaaa gtgtccgata tgtggagctg
    31441 gaatgtttgg gaataaatct ataaaatata aaaaagatgg gacgaagtat aaagacttct
    31501 attactatgg ttgtaagcat agacttatga atagaggaca taaatgtacc tataataaac
    31561 aaattagaga agaattgtta gatgatgccg ttgctgaggt aataatcaag ttagtaagca
    31621 accctaaatt tgcatctatg atacaagaaa aaattaacat gaaggttgat acatctgcta
    31681 ttgaaaatga gatagataat taccaaaatg aactaagaaa aagtcattct acaaagtata
    31741 agcttattga ggaaatagat aatttagatg tagaggataa acattataag agaagaaaaa
    31801 atgatttaga tgataggctt tatcgtatgt acgataaaat tgaggaatta gaaagtcagt
    31861 taatagaagc taaagcaaaa aaagaaacga tagaggttga gaaacttact ggtgataaca
    31921 tatataaagt attgatttat tttgacaagc tttataaagt catgaacgat gttgaacgca
    31981 ggcagttaat agaagcactc atttcagaaa ttcaaattta tgaagaaaag cagcctaatg
    32041 gacaatggct aaaatccatt acttttaagc tacctatcat tgatgaagat cttaatataa
    32101 gtttggaaaa tgatgagcaa gttgaaactg tagctaagat aatcaagcaa tagtaatgtt
    32161 tatatagatt atatactaat aatatattgt atttgccacc gatttgccac tgtaaagtaa
    32221 tttcggtggc aaatttagtc tacaatgtta tcaaaaatct ctaaagtttt atttttaatt
    32281 ctataaaact tattataatt gtaattttaa aagaaatcgt caatctacag ttgccagatt
    32341 ttatacaact tatttttttc ataggataat ttacataatc tatagttgaa tattatttaa
    32401 tatttttata ttataattca ttagcaaata taggcattac aaatgttgat aaacatgtga
    32461 aagcacatat ccacttataa ttattttttt aaaaaccata aaatcacctc ttcatttgtt
    32521 gtatgttttt ataacaaatg aggaggtgat ttccagacaa tttaaaaggt ttttgtagat
    32581 tgactaaaat ttatttttat tatttataat tcttaataat acatatgtac ctactgattc
    32641 catattaaac tgtataattt ttgttcatct ttttttataa ttaaaactac atagttttca
    32701 taatttctct tatatatata cttatcatct tttttaactt ctaacttata ttttttaaaa
    32761 tcttcttcaa ttttattttt attactttta ttaatgttaa cagaatctct tttaaattta
    32821 tcaatatatt tttctatttc attaatatta tctttgctta caactatacc accttttaaa
    32881 ttaaaaactt ttttgaaatt cttttcagaa aatccttgaa taaaataact ttgtccatct
    32941 ccattaatac tatcttgagt atcaaatact tcaatttctt tgttaggtat tggtatatca
    33001 aaattccaag attcctttag cattgaaata ttagctgctt ttactaaaaa aagattatat
    33061 aggatacttc ctaataacaa aacacctatt aatagtagtg tgattaatac aatttttctc
    33121 tttttcattt ttcgttcaca accttttgaa aaaacatgat ttaattcatc atttgaaggc
    33181 atgtcttcaa gaatagtttt tgttgtgata ggaatgcttt cttgaaacat atcatttgta
    33241 atatcaaaat cttttatttg attactcaat atccatttct tctaatattt cttttgat
//
LOCUS       DACTGW010000014        32948 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.014, whole genome shotgun sequence.
ACCESSION   DACTGW010000014 DACTGW010000000
VERSION     DACTGW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.014"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            98..559
                     /locus_tag="I9573_000560"
     CDS             98..559
                     /locus_tag="I9573_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT4795194.1"
                     /translation="MQNSVKQEPCDFSHGRFRFGGKIIRRNEKTKFDWLSRDKEQVEK
                     YINDPFCGVVCSCGFFYDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGV
                     LRLRDRYIKLGVKDVTCKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            1024..2238
                     /locus_tag="I9573_000561"
     CDS             1024..2238
                     /locus_tag="I9573_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4795195.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEKVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNIFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLWYANLSLEIKKMLSEDKGAFFSMYVDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(2658..3179)
                     /locus_tag="I9573_000562"
     CDS             complement(2658..3179)
                     /locus_tag="I9573_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4795196.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSYLQTEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            3535..4332
                     /locus_tag="I9573_000563"
     CDS             3535..4332
                     /locus_tag="I9573_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF348 domain-containing protein"
                     /protein_id="HAT4795197.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            4442..5032
                     /locus_tag="I9573_000564"
     CDS             4442..5032
                     /locus_tag="I9573_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HAT4795198.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNEETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFADFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            5098..6795
                     /locus_tag="I9573_000565"
     CDS             5098..6795
                     /locus_tag="I9573_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAT4795199.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGISLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     NVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            7009..7848
                     /locus_tag="I9573_000566"
     CDS             7009..7848
                     /locus_tag="I9573_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAT4795200.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            8365..10221
                     /locus_tag="I9573_000567"
     CDS             8365..10221
                     /locus_tag="I9573_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAT4795201.1"
                     /translation="MDIHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYRGGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIIDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALINKIFESDKGVVENKKVVTFELNVSINDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSENNIEKYNHKREISNELSKKLEIEFSDL"
     gene            10930..12147
                     /locus_tag="I9573_000568"
     CDS             10930..12147
                     /locus_tag="I9573_000568"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HAT4795202.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRSIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            12261..12755
                     /gene="cdeM"
                     /locus_tag="I9573_000569"
     CDS             12261..12755
                     /gene="cdeM"
                     /locus_tag="I9573_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HAT4795203.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGGNRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            13020..14285
                     /gene="hisD"
                     /locus_tag="I9573_000570"
     CDS             13020..14285
                     /gene="hisD"
                     /locus_tag="I9573_000570"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAT4795204.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(14488..15240)
                     /locus_tag="I9573_000571"
     CDS             complement(14488..15240)
                     /locus_tag="I9573_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT4795205.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANKKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(15358..15627)
                     /locus_tag="I9573_000572"
     CDS             complement(15358..15627)
                     /locus_tag="I9573_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795206.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            16102..17196
                     /locus_tag="I9573_000573"
     CDS             16102..17196
                     /locus_tag="I9573_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAT4795207.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LRERSINGVISMCLKETDYQYSLSNKKGGY"
     gene            17198..18148
                     /locus_tag="I9573_000574"
     CDS             17198..18148
                     /locus_tag="I9573_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAT4795208.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGLKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKIDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            18141..19325
                     /locus_tag="I9573_000575"
     CDS             18141..19325
                     /locus_tag="I9573_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795209.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKKSLMREMNNVSKGVIDNEDTPSGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKSLLEISSYFKSKGLKIGIDTFSPFISYFTGQDLKKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            19338..20834
                     /locus_tag="I9573_000576"
     CDS             19338..20834
                     /locus_tag="I9573_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAT4795210.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNSHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHTVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSEGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            20857..21807
                     /locus_tag="I9573_000577"
     CDS             20857..21807
                     /locus_tag="I9573_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4795211.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            21828..22790
                     /locus_tag="I9573_000578"
     CDS             21828..22790
                     /locus_tag="I9573_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAT4795212.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            23162..23794
                     /locus_tag="I9573_000579"
     CDS             23162..23794
                     /locus_tag="I9573_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAT4795213.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            24220..24297
                     /gene="kdpF"
                     /locus_tag="I9573_000580"
     CDS             24220..24297
                     /gene="kdpF"
                     /locus_tag="I9573_000580"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020062503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAT4795214.1"
                     /translation="MWFLAVVIIMLIIYLVYALINPEKF"
     gene            24338..26020
                     /gene="kdpA"
                     /locus_tag="I9573_000581"
     CDS             24338..26020
                     /gene="kdpA"
                     /locus_tag="I9573_000581"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAT4795215.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            26045..28102
                     /gene="kdpB"
                     /locus_tag="I9573_000582"
     CDS             26045..28102
                     /gene="kdpB"
                     /locus_tag="I9573_000582"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HAT4795216.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNVGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMIL
                     NLG"
     gene            28121..28747
                     /locus_tag="I9573_000583"
     CDS             28121..28747
                     /locus_tag="I9573_000583"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HAT4795217.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNASYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNIEVAKILGLI"
     gene            28984..29892
                     /gene="cysK"
                     /locus_tag="I9573_000584"
     CDS             28984..29892
                     /gene="cysK"
                     /locus_tag="I9573_000584"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAT4795218.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            29919..30509
                     /gene="cysE"
                     /locus_tag="I9573_000585"
     CDS             29919..30509
                     /gene="cysE"
                     /locus_tag="I9573_000585"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAT4795219.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            30669..30857
                     /locus_tag="I9573_000586"
     CDS             30669..30857
                     /locus_tag="I9573_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAT4795220.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(30911..31780)
                     /locus_tag="I9573_000587"
     CDS             complement(30911..31780)
                     /locus_tag="I9573_000587"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HAT4795221.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDMNKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(31977..>32948)
                     /locus_tag="I9573_000588"
     CDS             complement(31977..>32948)
                     /locus_tag="I9573_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795222.1"
                     /translation="EELFTNNLSEFYDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIE
                     YVFDVDISNDIKAKENPLINFNSLSLVVWSGETKHILQQMNILDLEDIHSYYMENNTN
                     ITIYENNTLQYKFDEESEDIKDSSIDIIEQMLILPDDLNYDKDEVENMKSRLAKINIK
                     YLQTLKEKDIKIKLINSNLTDEPEFSDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAK
                     IGYSNPSYANVHSSKNLELHETAHAIDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVA
                     HAYISKFIEEFFAESFVHYYLDEDSKNTLKENCPLTYDFLEKLELNY"
BASE COUNT        12837 a         3436 c         5845 g        10830 t
ORIGIN      
        1 ggattaagac taataacaat tcaaaataaa taacttatgt aagaaccgta ggaactacgg
       61 ggatagcctg tggagaatta gtaagacata ttttagtatg caaaattctg tgaagcagga
      121 accctgtgac tttagtcatg ggaggttcag atttggtggc aaaataataa gaagaaatga
      181 aaagactaag tttgactggt taagtagaga taaagaacaa gttgaaaaat atataaatga
      241 cccattttgt ggagtagtgt gtagttgtgg ctttttttat gatttagttc aagggttaaa
      301 agaaattgag gataaagaaa acttaaaaaa agtgccactt gatataccta tttacataat
      361 atctggagat aaagacccca taggaaaaaa tggtaaagga gtcttaagac taagggatag
      421 atatataaaa ttaggagtaa aagatgtaac ttgcaaactt tacaaagatg gaaggcatga
      481 attgttgaat gaaataaata gagaagaagt ttttgaggat ataatttgtt ggttaaacaa
      541 taaaatagaa atattgtaat aaaaaaatat aatgatatat ttgctttaaa tttatttata
      601 tataatattt gaaaagtgga ttatgtatat gcaaagtaga aaagagagag cctagaagtg
      661 attttttgct ataatgccat ttattgaata ggtgggtgtt attataaata aattattaac
      721 actcagtatt attatcattg tatatggaat ttattttatt taaaattcct tgaaagttta
      781 aatatagtaa gttgtagtaa agagaaaatc taaatattta aatgctattt aatgtgaatc
      841 tattaattag gttatactta aacttaatta atagatttta tatttcaagc caatgagata
      901 gggttttaga ttaaaatata accaatggtt aaattacaga cattttgtta actttccctc
      961 gatttactag taaatatttt ttttatatac ttaaattaca acagatgtac tagggaggta
     1021 tatatgcaaa ttaaaatagg taaagtcata cagcgtctta gaaaagaacg aaatttaact
     1081 caagaacaat tagcaaagtt tataggtgtg tcaacaccag cagtatctaa gtgggagagt
     1141 ggaaattctt acccagatat agaattattg cctttattag cagacttttt taatgtgtct
     1201 attgataagc ttttaaatta caagattgat ttaagtgaag aaaaagtaat gaaaatttat
     1261 aaagaattag aatcaggctt tgctagaatt gaaattgatt tatcaacaga agaaccaaaa
     1321 gaagaattta gacaagactt agaatctgta aaaaagcttt ctaatatgta tatagagaag
     1381 tatcctaaaa gctatttatt gaagctaagg atatgttcgc tgtatcagat gtattcatat
     1441 aaatttggta agagtgaact taatgacaga gtaaaagaaa caacgaatat tcttgaagat
     1501 atagtgagaa atacagatga tatccaaata aaagaaacag ctcttattat attatcaaac
     1561 gcatattgta tgttggaaga ttatgaaaag gcagagttgt atttaaatat gatacataag
     1621 tcaatagggg atacaagtgt taatttagct atgatatatt taaagcaaaa tagacttgaa
     1681 gaagcagaaa tattgcttca aaataaattg tttagtaata tatttaatat aagcatggat
     1741 tgtaagggaa taattaatgt atacaaaaat caatacaagg aattaaagaa aaaattggag
     1801 aatagaaatt ttaataaaaa tgcaattgag agagaaatgg agtatataaa aaataagttg
     1861 ctgtggtatg caaatctttc tttagaaata aaaaaaatgc tcagtgaaga taaaggtgca
     1921 ttttttagta tgtatgtgga ttatatggaa ttatctctaa tttttttatt ttttaacatg
     1981 aaagaagaag caaaaaaagc cttatatagt ctaaaagaaa tattagaaaa atatcctata
     2041 catgaaagtt tggatgtaag tcaaatgaga ttttttgata aggtagagtc taaaaattta
     2101 tatactttta atatatatac aaacttgtta attgtactta atgatgacag ttataatgag
     2161 cttagagaag aaccaatttt taaggatgta attgaaaaga ttttagatat ggaaaaaatg
     2221 ttgaaaaata aagaataagt aaaaatttaa tataggactt aaagattaat caaattaaaa
     2281 gaaagtgttt caaaataaaa atttgaatca ccttttttgt tggtcttaaa ccgttattta
     2341 gaaaaataga ggtttcaaaa tagaaatctg aattatctgt attattacac aaaaatagaa
     2401 acctaaattt ataaaaatag aataaagttt gttatataaa atgtaatttt gtctataaaa
     2461 tctcacatgt tttcttaact ttaagcgtga aaaagaggat gtatcatgaa ttttttagtt
     2521 catgatgcat ccttatttgt atgaaatcca atatatcttt attatttaaa caatggaaaa
     2581 gagtcttatt tctattttga gacatctctt ttatttaatt atgaagtttt atacagttca
     2641 ataaatagga attatattta gaaactcatg acattatttc ttagatagtg attaaattta
     2701 tcatattcca ttttagataa cttttctggg taagacatta caatcaggtt atgcccattg
     2761 aatttttcat ggtatggtgg atgaatgtgt tcataattat ttgtcttata ttttactctt
     2821 tcatagaacg ccttgcgata aattgaaatc tcatcaattg gaaggtcaat ttctaaaata
     2881 atagtttttc ctttcttatt taaaagctct aacgctcttt gtccatatct attattgcgc
     2941 atttctggaa aaatgcagaa atgttctaca tagataaagt tatctgtttc ccaacataaa
     3001 attatgccga caaactggtt tttatcataa attaaattga attgatattc tttatttttc
     3061 aaaatttctg tttgcaaata agattttctt tgttcatgaa atgggaaact aattttatat
     3121 aattccatag ccttgtaata taatttattt tcactggttc ttaaccgttc aaatttcact
     3181 ttactcctcc taatcacgaa aattccaatt tatttatgag ttcattttac catagtcagt
     3241 atttgcatac aatgtgtttt aagttgagtg tcatttcttt cttttattta tgcttatatc
     3301 tcatagacta agagataatt gcttaagaca taactaaaaa ctattatatt aagctttgat
     3361 aatctaaaaa cagtatattt atggactttt ctaggtctaa aagtaatctt aataatttta
     3421 gaagttacaa aaaagttaca taaatttgga aagctataat atacatatat aatataaggt
     3481 taaaactgta atgaataata ttaatttaaa atttgaacat ggaaggagat tattatggag
     3541 aaaagagaaa agaggataat aatatcatct ttattaagtg tatcaatttt aatgggtttg
     3601 gtaagtatat attctatatt aaataaagaa gacataattt taactgttaa gggtcaagag
     3661 caaaaagtat cttcctttaa gaaaacagtt gaggaacttt tggatgaaca gggtgtaaag
     3721 tataattctg aagataagat taatccaagt ttagatacag aactaaaaga tgatatgaaa
     3781 ataaaagttg ttaaggtaac taaaagtaaa aaagaagaga ttgaaaaaat tccatttgat
     3841 acaaagcatg taaatgatag taatttgtta aaaggaaaat ctaaagttta tcaagaaggt
     3901 caagaaggag aaaaaaaact agtctataat ttaacttacc atgatggaaa attagtcaaa
     3961 aaagtcttat caaaagaagt aatatctaag gagccaacta caaaaattat aaaatatgga
     4021 actaaagaaa aagtactaat agcatcaaga ggagcaaata taagaggagg caaacatatg
     4081 aaagtggttg ccactgcata tgcaggagat acaataacat ctactggtac aactccaaga
     4141 tggggtgtta ttgcagttga cccacgtgta ataccatatg gaacaaaagt atatatacct
     4201 aaactgggta tgacttttgt agcagaagat tgtggaggtg caattaaagg taatagaata
     4261 gacattttta tgaacagtga aggaaaagct tcaaattggg gaaggaaaag tatagatata
     4321 tatctacatt aaattagtga atagaataac tttacttgaa ggacaaaaaa gtataaaata
     4381 tgaattaact aaactgttag attattctta aaacataaaa atatatctaa ggggggataa
     4441 gatgaaaata gtctgcttag gagacagttt aacgtatgga tttggagtat cacgaagtaa
     4501 ttcatggaca aacattgtaa atgaagagac tcgattagaa atagtaaata agggtataaa
     4561 tggtgataca acaagtggta tgttagctag gtttaatgaa gatgttgtaa aaaactctcc
     4621 tgatatagtt tttataatgg gtggcactaa tgattttatt gctggtgcgg gaaatgaaat
     4681 tattaattct aatataatgg ctatggtgca tcagtcatat tcaaaaaata ttatacctat
     4741 aataggtata ccattaaaac cagatattcc aaatgttagg gaagattgga gttgttttac
     4801 tgattttaat atagtatctc aaaacttaga atcatatagt tattggataa aaaaattttg
     4861 tatgacattt aatactaatt tcgcagattt ttattcagaa tataataaaa atatggaact
     4921 agaaggatat aaaaaattat attttgatgg gttacatcca accaaagagg gtcatagaat
     4981 aatggcagac atttttatta attcaattaa taagtatata gaagagttat agattacatt
     5041 aaaatgcttg tttatgcgtc agttgcaata aatacataca gtagagaggg gtttattatg
     5101 gattatacta aaacttatga agagtggata aagggttcat attttgatga agatacaaag
     5161 ctagagttgg agaatataaa aaataatgaa aaagaaatag aggatagatt ctataaggat
     5221 ttagaatttg ggactgcggg tcttagagga ataattgaag caggaacgaa tagaataaat
     5281 aagtacacag ttagaagagc tacttttgga ttggcaaact atatattaga aaacacaaca
     5341 aaagaagaaa caagtagagg agtagtaata gctcatgata atagacacaa gtctagacaa
     5401 ttctgtatag aatctgcaaa tactttggca gcatgtggta taaaagcata tatatttgat
     5461 agtttgagaa ctactccaga attatctttt gctgttagaa gcttaaatgc aatcgctggt
     5521 atagtcataa cagcaagcca taatcctcca gaatataatg gatataaggt atactgggaa
     5581 gatggagcac aagttatgcc agagattgca aatgcaataa cagagaaagt aaatagtata
     5641 catgattata gtacaatacc aaccttaacg gaagctaata aaaatttggt agttttgttg
     5701 gacgaatcac aagatactaa atttatagaa gcagtaaaaa gccaaattat aagaaaagat
     5761 ttagttaaaa atgtaggtaa aagctttaag atagtttata cacctctttg tggaacaggg
     5821 aatgttccaa taagaagagc attgaaagaa gttggttttg aaaatataat tgtagttcca
     5881 gaagaagaaa atccagaccc aaattttgca gggcttgatt atccaaatcc agaagagaaa
     5941 aaagctttaa atagaggtat attactcgca aaagaaaaag gagcagacct tgtaatagca
     6001 actgacccag attgtgatag agtaggtgta gcagtaaaaa ctactacagg tgagtatgct
     6061 ttacttacag gaaatcaaat tggtggtatg ctgacacatt atataataga aggattaaaa
     6121 gaaaataaca aattaaaaga aaatcctaca ataataaaaa ccatagttac atcagaattt
     6181 ggagcagata tagctaaagc aaataatgtt gatgtattaa atgttcttac aggttttaaa
     6241 tttattggtg aaaaaataaa attatttgaa caaaataaaa atagaagtta tgtatttgga
     6301 tatgaagaaa gttatggtta cttagttggg actcatgcta gagataaaga tggtgtagtt
     6361 tcttcattgt taatatctga aatggcagca ttttactatt caaaaggtat aagtttatat
     6421 gatggtttaa tagaacttta taaaaagtat ggatttttca aagaacagac tatatctttg
     6481 actttaaaag gtatagaagg tgtagaaaag ataaaagaaa taatatctta ttttagagaa
     6541 aatcaaattg attatataaa tagtattaaa gtagttgata aaaaagatta taaaaatggt
     6601 atagataatc ttcctaaatc taatgtttta aaatactttt tggaagatga atcttgggta
     6661 gctataagac catctggaac agaaccaaaa cttaaatttt acatagctgt aaaaggagct
     6721 agtgatattg aagcagataa aaagattcaa ggtttgaaaa agtatataga tgacatggtc
     6781 gaaaaattaa aataaaatat tattatttta taagataatt ttgtaataat atttatttag
     6841 ctgaaaatta taatttactt tcattgagta gaatatggaa atgttagtag aattgttatt
     6901 ttcagctttt atttgtgtta tttttactta aataattata ttatagaatg aatctgagta
     6961 taattaatat tgtataatga aattaatttg atagaaaagg ggatatttat gtttggtata
     7021 gataagaaat tgattgaata tataattgca aaatccaatt acagtgaatt aagttcaaag
     7081 tcagcagaag tatctttctt cttaatgttg tccatatttc cttttttaat atttacaata
     7141 agttctatag cttatatacc gattcttcat ttaaataagt atatagcttt gtttagaaat
     7201 atgatgccag aaggtgcttt tgctgtgtta tcttcaatca tagtttctgc aatagacaat
     7261 agaaacctga aatttttagc agtcagtttt gtacttacta tgtggacatt ttcaagagca
     7321 gtaaaagctc ttattaaagg aatgaataga gcctataaag ttaaagaaac taggtctttt
     7381 tttaaaattt tatctatatc atttttattt acaataatgc ttttggtcct tattttttta
     7441 tctatgatat ttttagttta cggggaaaaa ataggttatt ttatttttaa tcttgtagga
     7501 ttggatgaga tatttataaa aatatgggat attctaagat acactgtagg tataataaca
     7561 ataatagtga tatttacact tttatataaa tacactccaa ataaaaagtt aactattaaa
     7621 gaatctgcgc caggtgctat ttttgctact tttgcttggt ttttagtatc atttttatat
     7681 tcttattata caaattacta tgctaactat gaagtaatct atggaagtat agctgaaata
     7741 attgtactta tgacctggat gtattttagt agttggtcta tcgttattgg ctatgaggta
     7801 aattcaagat tgtattttag aaagataaga catgaaatgt taaagtaaag tatttaaaat
     7861 aaaagagagt atttagtatt ataaatgtaa ttgaattaat aaagaaatat tgacatttaa
     7921 gttaaaaagt ctagaatttt tctgggcttt ttttattttt agtataactt gacaaatttt
     7981 atttaatggg atagttttaa tttataatca tctttgaaaa gatgactatt gataagtaag
     8041 gtataaaatt ggtaagtatc aaaaaatata ctaaataaaa attatagtcc taatgagtta
     8101 atttctgtat tttcaaataa tgcagtattt agtcatgtgt ttttatatgc gcctgtattt
     8161 ccaattacca atatattaga ataagctact gtaattagat ttagaaaaaa gcgacataaa
     8221 taattataaa caaataataa attacataaa attaaattta ttcacaagta tatcttataa
     8281 aatatgttat aattaataga aaattgtcat ataattaatt aagtagtatt attataaata
     8341 acattgaaag gtagtggtta gaatatggat atccataata aatatgtaaa ttttataaaa
     8401 aatatacctg tacctttttt atattgtaga attgtaaaga gacaagaaga tatagaatat
     8461 cgagtagaat atataagtaa gggtatgggc aaagtattac aattagaaga aggtatttgt
     8521 gataaaaata tactagatgt attgcctgta tttaagtcta aaaaatattt caaagagttg
     8581 ttctcaaatg aagtggattg tataaaaagg tacattccga cacttaaaaa ctggataaat
     8641 ataaaaaaac agataatagg ggattcatac ataattttgt attttggtaa aattgtattt
     8701 gattatagac agataatcga ttcctttgat aaaaaggaaa aagtagctta tattaaagac
     8761 gaagaaggca tatatataga ttgcagtgaa aatttaatac caatattaaa taacaacatt
     8821 aaaacaacga aagatatctt tggtaaaaat gatatagaag tatggggaga gaatacagga
     8881 aaattattta gagatgatta tagaggagga gtatctagta aaaaaagatt tttacaaaat
     8941 ctttttgaat atgaagaaac attttttatg gttgaaaaat attttttgta tgatgaagac
     9001 gaacttttag gtactatagg gataatagat aatataatat atagtggtta cagtaataga
     9061 aattataact caaaagattt aatgaagatg atagaacact caattccaga gaatatgttt
     9121 tataaagatg tgtatggaaa ttatattgga tttaattcag gttttttgaa tttagcttgt
     9181 atgaataaag aagaattatt agggaagaat agttataaaa tatcagaaga agaagcttta
     9241 ataaataaga tatttgaaag tgataagggt gtagttgaaa ataaaaaagt agttacattt
     9301 gagttaaatg taagtataaa tgatgaaaat aagtgtattg agattacaaa aaggccgttt
     9361 tttgatagct atggaagtgt tattggaata attggaacag caagggatat aagtagaaga
     9421 aaaagattag aagaggaaat ggataaaact aggatggaat tttttgcaaa tttatcccat
     9481 gaacttagaa cgcctataaa tttaatatca tcttcattac aagtgataga aaaaaaagaa
     9541 gcagatttaa tagaatcaaa tgatacttta aaaagaaatt taggtataat aaaacaaaat
     9601 ggaaatagaa tactgaggtt agtaaataat gttatagact ttacaaaaat gcaatcagga
     9661 tatttagatt ttaaaccaga agaatctgat ataattgcat ttatagagga gatatgtatg
     9721 tctgtagctg attttgcaag ccaaaataat atacaactta catttgatac agaaatagaa
     9781 gaattttcaa tgttatttga ttcagaaaaa ttggaaagaa taatattaaa tttactttct
     9841 aatggaatta agtacaataa gaaagatgga aaaataaata tatttttata tgtgaaagat
     9901 aatgtattta atatgaaaat atctgatagt ggaataggta taccaaaaga aaaaatagat
     9961 aaaatattta atagatttga acagattgac aatgaacttt cctacagagt taaaggaagt
    10021 ggtattggac tttcattagt gaaatctttg gttgaattgc atgagggaag tatatcttta
    10081 aaaagtcaac ttggtattgg aagtgaattt atagtttcat tacctgttag aagtgaaaat
    10141 aatattgaaa aatataatca taaaagagaa atcagcaatg aactaagtaa aaaattagaa
    10201 atagagtttt cggatttata agtttaaata taaaaaataa aattttaaaa aagtattgac
    10261 aattgattta gaagggttta aaataaatta caacataata aataaaaagg cgatgaatag
    10321 gaagagtaaa tctaattcga ttaccagaga gaagagtcca ttggctggaa gactttttaa
    10381 attagaggga ttgaaaacta cctaggagct actttctgaa gtcatctgca ttagtagatt
    10441 ttagtaagaa tagtcgttag ctgcgttaaa gtgtaaagtg agttttcatg atatatgaaa
    10501 actaaattag gtggaaccgc gggaataata ctctcgtcct tatgttataa ggatgagagt
    10561 tttttgttgt ttaaaaatat aaacagtaat cataataatt atagaaaata taaaagcgat
    10621 gaataggaag agtaaattta attcgactat cagagaggaa agttcacagg ctgaaagact
    10681 ttctaagttt gagaaattga aaactaccta ggagctgtgg tctgaagtta ctttaaaaag
    10741 ttttagtaag actaaccgtt aactgcgtta aagtgtaaag tggattttat caataaatga
    10801 taaaatcaaa ttgggtggaa ccgcgagtat agactcgtcc ctttatatag ggatgagtct
    10861 atttttattg caataaaaga ttgaaaataa aaaaaaatcg gaaaattata aatttaggag
    10921 gaaaataaaa tggaaaataa agtgaaaatt ggagtactag gatatggagt tgtaggaagt
    10981 ggacttatag atataataga taataataaa gaaaaaagaa gtatagaaat agttggaatt
    11041 ttagttaata atttggaaaa acataaggat aaaaaatatt ctaatataat aactaataat
    11101 atagatgata tatttaataa agatatagat atacttgttg aagtcatggg tggtttagaa
    11161 ccatcattat cttatataaa aaaagcatta aacaataaaa ttcatgttgt tacagcaaat
    11221 aaagatttgt tagctgaatg tggagatgag cttgcaaaat tagcaagtga aaataaagta
    11281 agtattaaat ttgaagcttc agtcgcagga ggtattccag tattaaaacc aataatagaa
    11341 tcactagaag gaaataatat agatagtata aatgcaattt taaatggaac tactaatttt
    11401 atattatcta aaatgtatga tgagaatcta tcttatgata tggcattaag acaagctcaa
    11461 gaattaggtt ttgctgaagc aaatccagag tcagatgtat tagggtatga tgctgctaga
    11521 aaactatcaa tattatcaac tctagcttat gacaatagag tatattggaa ggatgtgtat
    11581 ttagaaggaa ttacagatat agatgaaaaa gatattgaat atgcaaaaaa attaaattgt
    11641 aaaataaaat tgattggtca aagtaagtat gaaaatgata aagtaagtgc ttttgttaga
    11701 cctgttttag tagaaaaaga taatatcctt gctagaatag acaatgagtt caatgctgtc
    11761 atagtgaatg gagattcagt aggagaagtt tcttttgtag gtaaaggagc tggaagttta
    11821 gcaacaggaa gtgcggttta ttcagatgtt atagatataa tagataatag agtttcaagt
    11881 atagattctt ttactaaaga taaaatacaa gttaataaaa tagttagaga aaagtgtgga
    11941 gcacttctta gatttaaaaa atgtaataaa gatgaaattc taaatatagt tgagaattgt
    12001 ttagtaaaat tcgatatttt aaatgatgat gatgaacttg ctattatggt ttatgctgat
    12061 tctgaatatg aaataaacaa cagtttatgt ctaataaaag ataaaggata ttgcgaaaaa
    12121 atgaataaaa tgttgaaaat aagttaaaaa agaaaaaata agtttatatg taacatattt
    12181 tgacgatttt aatactatat agtatgagat gaaacaaaac atctcataaa aaattaaaat
    12241 tcaagggaga gatatgttat atggaaaata aaaaatatgc aaatggtggt tattcagaag
    12301 actggtatga aagaggagaa tctacagcta aatggttcca gaatgataga gaagaatatg
    12361 aaagagcagc atacgatgaa gatagagaaa gaagaggctc aaactgtgga tgttcagatt
    12421 caggaggaaa tagacctaga aactgtgaaa gatttagaag agaagctgag ataagagaaa
    12481 gagaagcaag agaagcattc tgtgaatctt cagagagaaa gaaagaagca ctagcatatg
    12541 aatgtgaagc tagaaaatta tgggaagaag cagaaaaata ctgggatgaa tattcaaaat
    12601 acaactataa aggaatcgaa tatttagcag aagctgctag attatttgat gaaggtatgg
    12661 aatgtgaagc tagaagaaat ggaaataatg gaggaaacaa taataattgt tgccataaat
    12721 gccataaatg taattgtaac tgctgtagaa aataaaaaaa ttcattgaca aatcaatagt
    12781 taaaatatat catattatgt ataataaaga gaaaattcag aaaaaaataa tgattagaat
    12841 aaatttaagt atatttatta gacaaatagt gctcagttag tgaggtgagt acaatatgat
    12901 taaactccca tcctctagct tttgatagag ggtgggagtt tttatttatt gatatatctt
    12961 aaatgaggat aatattgtaa aataaatgag tatgagattg taaaattagg aggtaaaaaa
    13021 tgaaaagtat taaaacatca agtatgaaag aaaaggtaga tgaaaagata ttaagagagc
    13081 aagtaagttc tataatagaa gatataagaa ataataagga tgttgcactt aaaaaatata
    13141 atgagaagtt tgatagaaat acaagagatg aatttagaat tactaaggaa gaaataaagg
    13201 aagcttataa gcatgtagat gacgaattta taaataactt aaagattgct gccaaaaata
    13261 taagagaatt tgctaaagct caaaaactaa gttttgaaaa cccttttgaa aaagaaattt
    13321 atccaggtgt aatcttaggt caaactaata ttccaataga gtcatgtctt gcatatgttc
    13381 caggaggtca gtatccattg ttttcaacag ctcttatgct tataatacct gcaaaagtag
    13441 caggagtaaa aagaatagtg gcatgttcac caactatgaa aaatactgaa aagataaatc
    13501 ctaaaacatt ggtagctatg gatatagcag gtgctgatga aatatatgca actggaggag
    13561 ttcaagctat agcagcattt acatatggaa cagaaaagat taatccagtt gacataattg
    13621 taggaccagg aaataagttt gtaactgaag ctaaaagaca atgttatgga caagtaggaa
    13681 ttgattttgt cgctgggcca agtgaagttc ttataatagc ggatgaaact tcaaatccag
    13741 tttatattgc agctgattta ttagcacaat gtgagcatga tttaaatgca agaggaatat
    13801 tattgacaaa tagcttggaa gtagcacaaa aagttgaaaa aaatatagaa agtatgctaa
    13861 aagatttgcc aacaaaggac atagcatata gttcatggga aaataatgga gagataatat
    13921 tggttgatga tatggaagaa gccattaaaa taagtaattt ttatgcacca gaacatttag
    13981 aagttgcagt aaatgagtat aatgatattt gtgatagatt aactaactat ggttctttat
    14041 ttattggaaa tctttctgct gaggtgtttg gtgactatgt ttcaggtacc aatcatacat
    14101 taccaacact taaagcatca agatatactg gtggtgtttg ggttggaact tttatcaaaa
    14161 cttgtacaaa gcagatattt aatgaaaaag ctatacaatc attagcacct gtagctgaaa
    14221 agctggcaaa agaagaggga ttatatgcac atgcaaaggc tgctgaggtt aggtttaaaa
    14281 agtagtaaaa aattttattt tggcagtcga gatttagtat tagactgaga taaagaaaac
    14341 gctgagaacc tgaataaata ctatattttg tgggcctcgg cgtttctttt atatctgtat
    14401 ataataggat aatactacct gtgtgtttaa atgctaactt aatatttctt ttctaaataa
    14461 tgtttatgaa atagttaata tttttagtta atacttgaaa aaaacttttg ctaactgtgc
    14521 aaattcatct gcatttgtca atatagcagg gtgtcctccc tgactaaaaa tgtgaatact
    14581 tgcttttgaa ataaaagttg ccatttgctt gtactcttct tcaagattat gacgacacat
    14641 ttcgtcttcc ttacttcctg taaataaaat tggcatttct aatttacaca attctttatg
    14701 aaataaaggg cgtttcttgt ttgcacattg caatagagct tttgtatcaa gattaacaac
    14761 attctcccaa tcttttccat gacaccattc atagaatttt cgtgcttgaa tatcttgctt
    14821 tgatttttcc cttccagtga ttagattatc tatgaagtta tcattaagag tacgtccatc
    14881 aaagctatct gctataattg cttcaactaa atcgggacgt tctaatccag tattaacagc
    14941 tgcccatgct cctccacttg ttccaattaa gccaacttta ccacaattca aatgttcaat
    15001 cagtgctatt gtctgtaagg cttcatcatg ccatatgtca ggagaaaatt gagaaactct
    15061 atcagactgt ccattaccta aaaaatcaat taagatacat ctaaaatttt ctacatataa
    15121 tggcacaagt gctttaaaca tattagaaga accagtatta ccatgcagga aaattatcgg
    15181 tttaccttgt ccatattctt tataaaaaca agaattacct tgatactgaa aatatgacat
    15241 atattttcct cctttttatt tgttaagtta attataacaa tataggtatg aatgaaaaac
    15301 ctcatgttct ttcttagaat aaaaatgtgc cacgcataaa acgtgacaca tttctaatta
    15361 taaattttgc tgtttaagta ttctttgtat gcttttctct gtcaaaaagt atttctctga
    15421 taactgcttg atactcattc cattttggaa atttatacag atttgagaat tacgagaagc
    15481 taagacttgt ttggtgtttg tgttttcacc ccaatgcttt ttgtgctctt gctttttggg
    15541 aatataaatc actttaccat ctatatattc ttgcacttgc tcaatcagtt ctttaggcag
    15601 taagtcaatc gaattttgat agctcatttt gcacctccta aataattact ctttaggatt
    15661 agcaaaggct actgctactc acaattaaaa ataaaatttt ttattatgta caatagcctt
    15721 tgctatgcac aagttccaat tcccataaac taaatactcc tttctggttt gaaaaattca
    15781 tctgaacatt gatttcgatt ttaagataga gttcaatgac aaatttacat ttttattata
    15841 tatattcaga tagataattg caactgtctt tgtatgagga tattttatta tagtcaatac
    15901 tataatatat atttatatca tcaaactaat atgaacagtt gaaaggaata tgaacaataa
    15961 aacttagtca ggaagaataa ttatgaagca gacaattgag aaatttgatt ttaaagtttt
    16021 attgaaaatt tattgacaaa tttaacttgt aaatattata ctaaacttaa taaataaagt
    16081 ttataaagta aggtgttgtc tatgaaaaat atagttggaa aaccgcaaac gataaaaaaa
    16141 gtaaatgaag atttaataaa aaacattata aaaaatgaag gtcctataac aaaaccagaa
    16201 atttcaagca ttactgaatt gagtttagct acagttaata agattgtgga gcaattagct
    16261 ttgaaaaatg aagttaaagt aagtggacta agtgaatcta ctggaggaag aagagctcaa
    16321 ttatttgaaa taaatgcaaa cttagaacac ataatagctt tatattatta tagaaattgt
    16381 tgtatagggg tagttgccaa tttattaggt gaaataattt atcaagaaga atttaatata
    16441 agaatggata catatgaaaa cgttaaccaa gatacttttt ttgttataga tactctaata
    16501 aaaaaggaaa aaggaaacaa tataagggct attggaattg gtgtcccagg agttgtaaaa
    16561 aaaggtgtag ttaaaaatat accaactata aaaagttggg aaggtataaa tttagaaaat
    16621 ttaataaaga gtaagtacaa tactaaagtg tttatagaaa atgatacaaa tattactact
    16681 attgggattt atcaaaaaca gtataaagac aaatataaaa atatggctct tatgtattta
    16741 gaagatggta ttggttcagg tatagttata aatggtgaat tgtatttagg ttctacaaat
    16801 tttgctggag aattaagtta tctaaatata gaatttggta agtataactc taacaactta
    16861 aatcttgaag attatattat gagtttaaga aaaagatatt tagaaagtaa agataataaa
    16921 tttaagaaag aaattttatc tataatatca aactttataa ggactcttgt atgtgtccta
    16981 aatccacaag ctatgattat acagtgtagt atattaacca agagtgacat ggagtttata
    17041 gaaaaagatg taagtaataa tgttggagaa gaaaattttc ctaaattaat aaaagtagag
    17101 tcattgagag aaagaagtat aaatggagta attagtatgt gtttaaaaga aactgactat
    17161 caatattcct tatctaataa aaaaggagga tattaatgtg aaatatttgg tttcaataga
    17221 tggaggggga acaaaaacta aattttgtgt aagtgactta gatggaaaca tacttaaaga
    17281 acataccact ggttcaacta attataagtc agttggaatt aaaaaaacat atgaaaatat
    17341 taataatgga cttaaaaaaa tattaaaaga tttatatatc gattatgacg atattgagta
    17401 cactgtattt ggtatatctg gttgtgattc tcctaatgat tataagatta taatggatga
    17461 aatattaaaa attgggatta acaaagaaaa aatatatctg gcaaatgatg cagttttagc
    17521 tttttatgca caagctgatt cccctggttt agtaattgtg gctgggacag gttcaattat
    17581 actgggaatt aaagaagatg gagaaatata tagagttggt ggatgggggt ataattttag
    17641 tgatttgggt tctggatatg atatagggag aaaattatta aagaaagtat tgctttattg
    17701 tgatgaatgt catgagtata gtgatttatt tagttgtgtc ttggatttct ttggagcaaa
    17761 tagttttgaa caacttgcat atatgattac tgatataaac aataatgttg aaatagcaaa
    17821 cctagcttca ttagtgattg attgtgccga acaaggtgat aaattagcta ttgaaatatt
    17881 aagagaatca tcaactgaac tatctaaatt agctcaagta atattgagaa aaatatctaa
    17941 tccaatgaaa atagataaaa gcgaaataaa tatagttttg tctggtggta cattaaataa
    18001 aactgtatat gctcaaatgt taatgaataa tttaaaaaca gaaaatacag ataaaaattt
    18061 aaaatttata tctcaagaaa atcagccagt atatgggggg ataagacttg ctatggcatt
    18121 agctaatagg agagttaagc atggatagaa aaaaggtaat agtacaaatt tttactggag
    18181 gttttaaggc taaagaaata aagtttaatg atataaaatg taaactacta gaattattgg
    18241 actgtattga tatagaaaag gttatcatgg gatggagtgt agatgaagaa ttatatgtag
    18301 aaactaaaaa actattaaat aaatataatg ttgaactata tctttggttg ccatgttttt
    18361 ctgaaatagg attacttgaa aaaagtaatt tactaattga ttatatgggg aaggaagtca
    18421 agggctatgc tttacaagag gatgaaaatt ttgaatttta ttgcccaaat aataaggaaa
    18481 atataaaaaa tataaagaaa atatatagta cttattttag tgatattaaa tttgatggaa
    18541 tttttttaga taaaataagg tatggttctt tttcgaataa actaaatggt gtttttaatt
    18601 gtttttgtaa agattgttta aatatatatg aaaaaaataa tattgataaa aagtcgttaa
    18661 tgagagaaat gaataatgtg tctaagggag ttatagataa tgaagatact ccatctggta
    18721 taacagaata cattaatgga aaatacaaat ttaaaaataa aatatgggaa gatttttttg
    18781 aaacaaaaaa tcaaaatata tacaagtcat tattagagat aagtagttat tttaaaagta
    18841 agggattgaa aataggtata gatacttttt ctccttttat ttcttacttt acaggtcaag
    18901 atttaaaaaa attacaagaa gtagcagatt ttataaaacc aatgatgtat agaactacaa
    18961 aagctccagc tggtttacca tttgaatttg aaaggtttat tgatgaatcc atacataaga
    19021 ataaagagat tggaaaaaga aatttcttag atattttagg aattaaagag tatgaaggag
    19081 atgtatatcc tatagaattt gtactagagg aaatcgattt tatgacaagg aatcttaata
    19141 aagatatata tgtaggagtt gaaataaata aaaaggaaat tatagctcct gttacaccta
    19201 agtacataag tgaaaatatt tcaaatataa ataagacaac agtaaaagga tatgttttat
    19261 cttgggattt gttatcagca ccaaaagaaa atattgatga aattataaaa tggtttcgga
    19321 attagtgagg tgatatagtg agtaagatat tagaaatgca acaaatatat aaaagttttg
    19381 gaaaggtaga tgttttaaaa gggataaatt ttgagttaga aaagggagaa gtaagagctc
    19441 ttttgggagc taatggagca ggaaaatcta cacttattaa aattttagga ggagttcatt
    19501 ctcaaactaa aggtaaagta attttgactg gtaatgaaat aatgtttaaa aattcacatc
    19561 attctaaaga aaatggtatt agtataattt atcaagaatt aagcttgata ccaacgctta
    19621 cagtacttga aaatattttt cttggtagag aaattttaaa aggaagtgtg tttttaaaca
    19681 agaaagacat gcttgaggaa tatatgaaaa tttgtgagga ttttgatttt aatatagacc
    19741 cacatacagt agtttctaat ttaagtgtag caaaccaaca aatggttgag ataatgaaag
    19801 ctgtttcatg taattctgaa attatcataa tggatgaacc tacaacatca cttactaata
    19861 atgaaaaaga tgggctattt aaaataataa gtagactaaa gtctaaaggt aaaacaatag
    19921 tctatatttc tcacatatta gaggaagttt ttaaagtatg tgatagtgca agtataatga
    19981 aaaatggaat tatgattgga acttatgatg tagaggattt aactaaatta aagataactc
    20041 aacttatgac aggaaatgat aatcacaaac ttattgagaa gaaggattat acatttgcaa
    20101 agtatgaaga gccaccagta ctagaagtaa agaatataag ctggtctaac aaaataaaaa
    20161 atgtttcatt taaagtttat agaggcgaag ttgtaggtct tgctggtctt gtaggttcaa
    20221 gaagaactga aattataaat ttgatatttg gtatagataa taaagatagt ggagacattt
    20281 ttatcaatgg taaaaaagta gacataactt ctcctaaggt tgctataaaa aacaaaatag
    20341 gattgattcc tgaagataga aaaaatctag gattaatttt agaacaagct atatacacaa
    20401 actcatcatc agtacaaatt gataaattca agaaattttt gtttatagat agaaataaag
    20461 aaattgagtt ttctaaaaaa ggtgttaagg aattaggaat aaaagttagt gatgtaaagc
    20521 aagaagtaaa agagcttagt ggtggaaatc aacaaaaagt agtagtttct aagtggttag
    20581 agaaagattt agatttaata atatatgatg agccgacaaa gggaatagat attgctgcaa
    20641 aagaagatat tttcaataca atcaaagaat tttcaagtga aggaatagga gtaatattta
    20701 tatcttctga tttagaagaa gtaataagag taagtgatag aattttagta actagagaag
    20761 gttctattgt agatgattta aaaaatgaga atatacaggt tcaagatata atgaacagta
    20821 tatttaatgt ataaaaaatg aaaatggagg actgagatga agaaagcttt tgagaagtta
    20881 aatttaagag attatggagt attattaggt tttttagctt tatgtatagc tataagtatt
    20941 gcaactccat catttttagg taaacaaaat atacttaatc ttttacgaca atcttctata
    21001 attggaataa tttcagcagg tatgactttt gttataatat ctggaaactt tgatatatca
    21061 gttggagcag tagcagctct tgcaggtgct ataacaatga aatttgcgac tggtggaact
    21121 aatttgtttg catccatgct cttaggtgta gcgatttgtg cagtaatagg attaatcaat
    21181 ggtatagtag ttgcaaagat aaatgtacca tcattaatag caactatggc tatggtcact
    21241 attgtaagag gattgctgtt gatgcttaca ggaggatatc caattactga aaatatacca
    21301 atacttgatt acattggaaa tggatatttg tttaatatac caataccagt agtaattttc
    21361 tttatagtgg tgttagtttc atttattgta cttaataaaa ctaagtttgg tagatatgtt
    21421 tattctgtag gagggaatca agaagcaagt aaacttaatg gtataaatgt agattctcac
    21481 aaagtgaaag tgtttattat taatgctgta cttgcaggaa tagctggaat cgtactaaca
    21541 ggaagacttg ggactgcaac tgctattgca ggtgaaggat atgatatgga tgcaattgca
    21601 tctgtagtaa taggaggtac atcagtggca ggaggttctg gttctgtact taaaactgtt
    21661 ataggagtat tacttatgag tgttataaac aacagtttta atcttcttgg aatagatgta
    21721 tatttccagt acatatttaa aggattaata attttagcag cagttggatt tgattcttat
    21781 agcaagaaaa aacttgcttc aagataaaaa ataaggggga tttttttatg aaaaaattaa
    21841 tgaaacattt agcattgttg ttatcacttg ttatgatatt tggtttagtt ggttgttcaa
    21901 atggtggtga caaagaaaaa aatggagaaa agaaaatagc agtattacta ccaggttcaa
    21961 caggttactt tgtagcaact aaacaaggta tagatgcaaa atctaaagaa ttaggtgtaa
    22021 gcgtggaata tgcagatgct caatgggatg ctagtaaaca attatcacaa gctgaagatt
    22081 ttatggctaa aggggtagac atgattgcat tatgtggagt tgattctgct gtaagtgaga
    22141 gaatagttaa agctgctaat gattcagacg tccctatagt agcatttaca aattctatag
    22201 gaagtaaccc aactggagaa tttaaaggtt tagttacata tataggacaa aatgaggagg
    22261 aaacaggagc actaactgga aaaattgcta aaaacttatt aggagaaact ggtgggaaag
    22321 ctgtattaat agaaggagta ccaggaacta ctccacaagt attaagaaaa aaaggtcttg
    22381 aaaaagaatt aaaagatagt aatatagaaa tagtttataa tcaaacttca aattgggaga
    22441 aagagcaagc tttaaaagta acagaagatt taattcaaaa gaaaactgat tttaatgtta
    22501 taatatgtca agatgataat tctgctacag gtgcaggtca agctttaaaa gatgctggtc
    22561 ttaaggataa agttaaagtc ataggtttgg gtggaagtaa agatggatta aaagctataa
    22621 ctgatgggtt aatagatggt acaacttata tgtctgctgt agaagaaggt ggattagtca
    22681 tagaaaaggc atctaaattc cttaaaggtg aaaaaataga acctgttaca caaattaaac
    22741 aagttgaagt taacaaagat aatatttcag agttcaaggg tgaatggtaa attgtcatag
    22801 ataataaata agatttttag agttacttta gaaactatat aaagtaactc tttttttaat
    22861 ttaatttagt gataaattat tgaatagtat ttctaaacaa ataaaaaatt aatataaaca
    22921 aagacttaat tatactaaat tataagtaaa actaatataa acttatggaa aaaaggaaaa
    22981 cgtctaaagt attggaatta ctatagtgta ggtgtggtat taaaaaaaca taaaaaaaca
    23041 taaaaaaatt gttgaaaaaa atatagaaat tttgtataat taaagatttg aaaagttcgg
    23101 tatacaaagc caattctttt gtatataata tatataaatt cagaaaaagg aagggatatg
    23161 tatggccagg cggttatttt tagaaaggat tggacttata tttttaggtt cagcaatatt
    23221 agcatttgga gtttataatt tttattattt aaacaacata acagaaggtg gagtgttagg
    23281 tattttacta ctgttaaaaa atttattcaa tatacaacca gcaatagcaa atgttgtaat
    23341 agatggttta ctacttttag taggatataa atttttcggt aaaaaattcc ttatatattc
    23401 aatagtagct tctataactt tttcagtgtt atatgactta tttgaagcta taggacctct
    23461 agtaccacaa tcacaaaata tgcttttatc aactattttg gcaggtgtaa ctgttggaac
    23521 tggtgtaggt atagtagtaa aagcaggttg tgcatcagga ggagacgatg ctttagcatt
    23581 agtaatttct aagactacat ctttgaacat agggcaagtt tatttagcaa cagatgttat
    23641 tgtattgttg ttatcattat tttatctatc agcgtttgat atcttttatt ctttgattgc
    23701 tgttacaatt agtggcaagg taattgattt tatttattat catggtaaaa gtttggatat
    23761 ggatataagt aatgatatag ttccagaatg ttaatataaa ttaaataact ttagatttta
    23821 ttaggggtgg cagatttgcc accttttttg tgtctaaatc tttatagaaa gtataatccc
    23881 atctaggttt ttgcaaatct caaatataga attatgttgg aatgactggg aatacgattg
    23941 ctagatgtgc attttaaaaa cccaatttta tatttatgaa atactaatca ttaatggatg
    24001 tttcttaatc ctatctttat acctaaaatg ataagataaa tgaagtagca atacaaaact
    24061 aaaatttgta aaagagatta aattaaatgg aggagctaaa atgttggata tagttatgat
    24121 agcattgtta ttcataggat ttgtttccat gaagttactt gctgattggt gtgggaaaca
    24181 aactgagaaa aaataatgta atgtatggag ggattaccta tgtggtttct ggcagttgta
    24241 attataatgt taataatata tttggtatat gcgcttatta atcctgaaaa attttaaaca
    24301 gtataaggct aataagtaag ttgaattggg gggaacaatt tttatggtgc agatagtaat
    24361 agtgttagcg atttttatga ttttagtaat acctatgggc aagtatttat atcatatagc
    24421 tactaatcaa aaaacatttg gagatagact atttgataaa gtggacaatt ttatatataa
    24481 ggtatgtagt atagataaga aaaaagaaat gaactggaaa cagtatgcat tagctttatt
    24541 atttacaaat gcagttatgg tatttatagg atatatcata cttagaactc aaagtatgca
    24601 tatttttaat ccaagtggaa ttaaaagtat ggaacaagga ctatcattta atacaataat
    24661 aagttttatg acaaatacaa atcttcaaca ttattcaggt gaatcaggac tttcatactt
    24721 tagtcagatg acagtgataa tatatatgat gtttacatca gcagcaacag gatatgcggc
    24781 tgctatggca tttgtaagag gtctagtcgg taaaaagaaa acattgggaa atttttatgt
    24841 agatttaatt aggataacaa caagagtact tttaccagga gctttaatta ttgggcttat
    24901 attagttaca caaggagtac cacaaacttt tgctgggaca gaaacagtga ctactataga
    24961 aggaaagctt caagatatag ctagaggtcc tgtggcagct cttgaatcaa taaaacattt
    25021 aggaacaaat ggtggtggat tctttggttc aaattcatca cacccatttg aaaatccaac
    25081 aataatatca aatatagtag aaatattatc tatgatgata ttaccaggag cttgtgttgt
    25141 tgcattcggt cacatgataa agaataaaaa acaaggttgg gttgtgtttg gcgcaatgtc
    25201 aatcatattt ttaataggtc tagtagtgtg ctttaaagca gaaagtgcag gaaaccctat
    25261 tttatcacaa ttagggctta atcaaagtat gggaagtatg gaaggtaagg aagttagatt
    25321 tggtatagca caatcatcat tattcacaac agtgactact tcctttacga caggtacagt
    25381 taataatatg catgatacac tgactccttt aggaggatta gtaccacttt taaatatgat
    25441 gctaaatgta gtatttggtg gtaaaggcgt aggtctgatg aatatgttga tgtacgctat
    25501 aatagcagta ttcttatgtg gattaatggt aggaagaaca ccagagtttt taactaagaa
    25561 aatcgaagga aaagaaatga agcttattgc tttacttatt atattacatc cacttttaat
    25621 actaatgttc tctggtcttt cagtagcaat accagcaggg cttgaaggaa tttcaaatcc
    25681 tggattccat ggattatcac aggtattgta tgagtttgca tcttcagcag ccaataatgg
    25741 ttcaggattt gaaggtctag gagataacac tatgttttgg aatataacaa caggtatagt
    25801 tatgttcttt ggaagatatg tgtctattat agtactacta gcaatatcaa gtttgttagc
    25861 atctaaaaaa gctgtaaatg aaagtatagg aacacttaga acggataatt ttacatttac
    25921 aatagtttta gtacttgttg ttttaattgt tggtgcttta acatttttcc cagccttagc
    25981 gcttggacct atttcggagc atctagtgtt gtggcactaa aaaattggga gggagtaaag
    26041 ttttatggaa actaaaaaaa caaagtttat tacaaaagat attttcaaaa cttccattat
    26101 agaagctttt aaaaaattaa atcctaaata tatgatgaaa aatccagtta tgtttgttgt
    26161 agaaattgga tttatagtaa cattgatact tacaatagta cctagtttgt ttggtgatga
    26221 aggcaataat ttaagagctt ataatgggat tgtaacaata atcttattta taacagtcct
    26281 ttttgcaaat tttgctgaag ctgtagctga gggaagaggt aaagctcaag cagatacact
    26341 taagaaaact agaaaagata cagtagcaag ggttatagat acgttaggaa atgaaaaaat
    26401 gataagtgca agtgagctaa aacttggtga tattgtttta gtaaatgttg gagaagttat
    26461 cccaaatgat ggagaagtta tagagggcat agcttctgtg gatgagtcag caataactgg
    26521 agaatctgca cctgtcatga gagaatcagg tggagatttc gcatctgtta caggtggtac
    26581 tacagttgta agtgactggt taaagataag aataacaaca aaaccaggag aatcgttctt
    26641 agataaaatg atagctcttg tagaaggtgc ttcaagacaa aaaacgccaa atgagattgc
    26701 tttaaatact ttgttagtag ggttaacact tatatttatg gttgttttaa tatctcttta
    26761 tccaatggca aactatgcag gtgtaaagat tccaatttct actatgatag ctcttttggt
    26821 atgtttaata ccaactacta taggtggatt gttgtcagct attggtattg caggtatgga
    26881 tagagtaact agatttaatg taattgcaat gtcaggaaaa gcagtagaag cttgtggaga
    26941 cgttgatact atgattttgg ataagacagg tacgattact tatgggaata gacttgcatc
    27001 agaatttata gcagttggaa atgcagattc aaaagattta ataaattatt ctgttatgtg
    27061 ttcattaaaa gatgatacac cagaaggaaa atctatagtt gatttaggta tgaaattagg
    27121 ttcaacaggt aagacaaaag aggcagagga agctgagttt gtagaatttt ctgcacaaac
    27181 tagaatgagt ggtattaact tagctgatgg tagagctata agaaaaggtg cttatgatag
    27241 cataataaaa agagtaaaag aatctggtgg tacagtgcca gaagatttag aagaaaatgt
    27301 aaatcgagta gctaaactag gaggaacacc tttagtagtg tgtgtagata atgagatata
    27361 tggaattatc tacttaaaag atacagtaaa atcagggctt gtagaaagat ttgctagact
    27421 tcgagagata ggaataaaga caataatgtg tacaggggat aatcccttga cagcagctac
    27481 aattgcaaga gaagctggag tagatggatt tattgcagaa tgtaaaccag aagataaaat
    27541 agatgctata aaaaaagaac aattagaggg taaaatagtt gcaatgacag gtgatggaac
    27601 aaatgatgct cctgcacttg ctcaggcaga tgtaggtctt gcaatgaata gtggtacaac
    27661 atctgcaaaa gaagcagcaa atatggtgga tttagattca gacccaacta agatattgga
    27721 agttgttgaa ataggaaaac agcttttaat aactcgtggg gctttgacaa catttagtat
    27781 agcaaatgat gttgcaaaat attttgcgat tataccggct atatttactc ttgcaatacc
    27841 tcaaatggag atattaaatg taatgaaact ttctacatca tttagtgcga tattatcagc
    27901 tttaatattt aatgctataa ttatcccatg tttaatacct cttgcaatga agggagtaaa
    27961 atatagacct atgaaatcac aagctatgtt aatgagaaat atgcttatat ttggtcttgg
    28021 tggagttata gtaccatttt taggaataaa attaattgat ttattgatta cacctttagt
    28081 aatgattctt aatttaggtt aataaatgga ggatataaaa atgaaaacat taaagccagc
    28141 attacttgta agtattgtat tgttagttgt ttgtggactt gtatatccac ttgttttgac
    28201 aggtgtaagc caagttgcat ttaaagataa agccaatgga agtatgatag aagtaaatgg
    28261 tgtaaaagta ggttcagaac ttattggtca aagctttaca gatgcgagat tctttaaggg
    28321 aagagtatca tcagtaaact ataatactta tacaaaagaa gatttagtac cagataaaga
    28381 tggaaatgca tcttatggag gtgtatcatc agggtctttt aattatggag ctacaaatcc
    28441 agaacttcat gatagagtac aaaaagatat agaaaagttt ttaaaggata atccaactgt
    28501 caaaagagag gatataccaa cagatttact tacagcatca ggttcaggac ttgaccctaa
    28561 tataagtcca gcctctgcaa aaattcagat accagctata gcaaaagcgt ctggaatatc
    28621 agaaagtaaa ttacaaaaaa ttgtagatga taatacttcc aaaaaacttt ttggagtttt
    28681 aggtgaagat agagttaatg tactaaaggt taatatagag gtcgcaaaaa tattaggtct
    28741 tatatagtat tttgtggaat tttagtaaca aaatattact caagatttaa tttaaaggtt
    28801 aaattttaaa gagtgaaaat caataatgat tttcactttt ttatttgaat attactaata
    28861 tttggaataa agtgattgta agtataaaat ccaaaaaaag gttgcattat caaaatgata
    28921 atactataat atatttatcg ttatcaattt gataatagat ttggacatat aggaggaaat
    28981 agtatgttat ataataacgc attagagttg ataggaaaca cacctgtagt aaggctgaat
    29041 aatttaggct gtactaatgg gtgttctaac atatatgtaa aattagaaaa agtaaatcca
    29101 gcaggaagta taaaagatag agctgtttat gaaatgttag agggcttaga gcaaagagga
    29161 gaattaaaaa agggagatgt actagttgaa gcaactagtg gaaatactgg tattgcatta
    29221 tctatgatag gaaaattaaa aggatatgaa gtagtaatcg taatgcccga aacaatgagc
    29281 gttgagagaa gagatttaat gaaggcttat ggagcaaatt tggtgcttac agatggtaaa
    29341 gctggaatgg caggttccat agaaaaagca aatgaacttt taaaagaaaa tccaaactat
    29401 aaaagcttaa aacaatttga aaataaagat aatccaaatg cacattataa aacaacagca
    29461 gttgaaatta tgaatgatgt caaagattta gatatattcg tatgtggagt tggaacaggt
    29521 ggtactttaa taggaactgc aagatattta aaagaacaaa atccaaacat aagagtaatt
    29581 gcacttgaac caaaaaaatc tccagctata tcagaaaaca agactggacc tcataaaata
    29641 caaggaatag gtgctaactt tgttcctgaa aattatgatt ctagtgtagt tgatgaagta
    29701 atattagtag atgatgaaga tgcatttgat acagttaaat tacttgcagc aaaagaggga
    29761 attttagtag ggatatcttc tggagcaaat atatttggtg ctatagagat ggcaaaaaag
    29821 tatccagata aaaagatagt tacagtagct ccagatggag ttgataagta tatgtctatg
    29881 ggaatatttt aataatttta tttgagggag tgaatacttt gtttaagaaa ataaataaag
    29941 atattgaata tataatgaag aatgatccag cagctaggtc taagatagaa gtttttttac
    30001 tttatccatc agttcatgca atgataatgc ataggatggc tcatgctctt tataagaaga
    30061 agaagctttt tacagcaaga ttaatatccc agatatccag atttatgact ggcatagaaa
    30121 ttcatcctgg tgctaaaatg ggggaaggta tattgataga ccatggaatg ggtgttgtaa
    30181 ttggagagac agctgaagta ggtaataggg tgacaatata tcaaggagct actttaggag
    30241 ctacaggaaa agatactgga aagagacatc caactgttgg tgatgatgta ttaattggtg
    30301 ctggaacaaa aatactagga cctttaaata taggttcaaa ttcaaaaatt ggtgcaaatt
    30361 ctgttgtcgt taaggatgta cctaatggtg caacagttgt tggtatacca gcaaaaatag
    30421 taaaaataag aaacctagaa cctgtaaaaa agaataaaaa agaagtgtcc tatgagtatg
    30481 atgaattaga taatgtatat tatatatagg atttatataa ctataaaata aactttataa
    30541 aaattatttt aaaaggtatt tttttcaaat ttgaggacat aataaaaaag aactgaatgt
    30601 tacagttttg gttcatcaat ctttgataaa caagatttta aatttgtagc gtctaggagg
    30661 attttattat gaaagcaaat gttaatcaag atacttgtat aggttgtgga ttatgtccat
    30721 caatatgtcc agaagtattt gatatgaagg atgatgggaa atcacatgtt atagttgatg
    30781 aagtaccaag tgatgctgaa gaatcagcag cagaagctag agaaagctgt ccagttgatg
    30841 caatagatgt gcattaataa aataaatgcc tataaaaatt gaattttcaa tttttatagg
    30901 catttgtctt ttattcagtt agtatttcaa cccctgtttc tgtaactaaa accatacatt
    30961 cccattgagc agataattta ccatcttttg tataaatagt ccaattattt tcttcatcaa
    31021 caaaaatttc gtgagttcct tcatttacca taggttctat tgtgaatatc atcccaggaa
    31081 ccaatagcat ctcagtccct tttttagata cataacttac aaaagggtct tcatgaaagc
    31141 ttaatccaac tccatgacca cctatttctc taacaacaga gtatccattt ttctttgcat
    31201 aagagttgat agcatcacct atatctccta aaaatcccca tggtttggca acttcaagac
    31261 ctttttggac acattccttt gtaacttgaa ctaatttttt tgcattttca ttaacattac
    31321 ctatcataaa cattcttgaa gcatctgaat agtatccatt aaaaatagta gaaacatcta
    31381 catttataat atctccatct tttaagataa tgtccttgct tggaatacca tgacaaactt
    31441 cattatttac tgatgtacat acacttttag gaaatccatt atagtctagt ggagcaggga
    31501 tagctccatt ttgtagtgta aagtcatgaa ccaacttatt catatcttct gtagacattc
    31561 caatttcgat tttatctgaa atataatcca aaatttttgt gtttattaca gcactttttt
    31621 ttatttcctc aatttgactt tcatttttta taatatcatg agttggtaca atatgaccta
    31681 acttagcaaa tgaatctatt ttattatcca tatccatatg acatttttta tattttttag
    31741 tacttccaca ccaacagcta tcatttctat ttaacttcat aacttttcat ccttctttta
    31801 ttatttattt aaattatggg ttacataaac tattttaaaa cataaccatt ataaatatta
    31861 taacataaat actaagaaat cctttataga tagtgattta ttcaaagtat aaattattat
    31921 atggttattg ttgatgaaaa gaggatagtt aaatgtatta ttggatatta gcgcacttaa
    31981 tagtttagtt caagtttttc aagaaaatca tatgttagag gacaattttc ctttaaagta
    32041 ttttttgaat cttcatcaag ataataatgc acaaatgatt ctgcaaaaaa ttcttctata
    32101 aacttgctta tatatgcatg tgcaacttgt ttagggtcat aaagcttata tctttcctga
    32161 gcaaatactt ccataaattc ttcagaattt gattttttat ttaaaacatt tttatctata
    32221 gcatgtgcag tttcatgtaa ttctaaattt tttgatgaat gtacatttgc atagctaggg
    32281 ttactataac ctattttagc tactatagaa ttatttctat atataccagg tacatctttc
    32341 caagtcttgc ctgatcttac ccaacatgga ggtaattgat attttaaatc actaaattct
    32401 ggttcatcag ttagatttga atttattaat tttattttta tatccttttc tttaagtgtc
    32461 tgtaaatatt taatatttat ttttgcaagt ctgcttttca tattttcaac ttcatcttta
    32521 tcataattca agtcatcagg aagtataagc atttgttcaa ttatatctat gctactgtct
    32581 tttatatctt cagattcttc atcaaattta tattgaagag tattattttc atagatagtt
    32641 atatttgtat tattttccat ataataagag tgaatatcct ctaagtctaa aatattcatt
    32701 tgttgtaaaa tatgttttgt ttcgccactc catactacta aagaaagtga attaaaatta
    32761 attagtggat tttcctttgc ttttatatca ttgcttatat ctacatcaaa tacatattca
    32821 attgagtctt ctatgttaag actatctgta ttctttttaa tatataaata tgcttgtttt
    32881 aagtctatat aaatctcatc taaagattca tcataaaatt ctgataaatt attagtaaag
    32941 agttcttc
//
LOCUS       DACTGW010000015        32601 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.015, whole genome shotgun sequence.
ACCESSION   DACTGW010000015 DACTGW010000000
VERSION     DACTGW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.015"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(123..962)
                     /locus_tag="I9573_000589"
     CDS             complement(123..962)
                     /locus_tag="I9573_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAT4795223.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(986..1744)
                     /locus_tag="I9573_000590"
     CDS             complement(986..1744)
                     /locus_tag="I9573_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT4795224.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1791..2264)
                     /locus_tag="I9573_000591"
     CDS             complement(1791..2264)
                     /locus_tag="I9573_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4795225.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDEHLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2293..2709)
                     /locus_tag="I9573_000592"
     CDS             complement(2293..2709)
                     /locus_tag="I9573_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT4795226.1"
                     /translation="MIHMTGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRRLEDRIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2756..3808)
                     /locus_tag="I9573_000593"
     CDS             complement(2756..3808)
                     /locus_tag="I9573_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAT4795227.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGTIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPMTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4204..4884
                     /locus_tag="I9573_000594"
     CDS             4204..4884
                     /locus_tag="I9573_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAT4795228.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5017..5337)
                     /gene="sugE"
                     /locus_tag="I9573_000595"
     CDS             complement(5017..5337)
                     /gene="sugE"
                     /locus_tag="I9573_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HAT4795229.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5719..6546
                     /locus_tag="I9573_000596"
     CDS             5719..6546
                     /locus_tag="I9573_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAT4795230.1"
                     /translation="MNFYKIAVCQMMTTENKIENINHAVDMVTEAAINGAKIIVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEYGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGVEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            7367..8041
                     /locus_tag="I9573_000597"
     CDS             7367..8041
                     /locus_tag="I9573_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4795231.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDSLKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            8029..9189
                     /locus_tag="I9573_000598"
     CDS             8029..9189
                     /locus_tag="I9573_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4795232.1"
                     /translation="MDKISNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNINMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNPHFGMGLHISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            9406..9933
                     /locus_tag="I9573_000599"
     CDS             9406..9933
                     /locus_tag="I9573_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAT4795233.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            10219..10887
                     /locus_tag="I9573_000600"
     CDS             10219..10887
                     /locus_tag="I9573_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4795234.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLKINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            10890..13358
                     /locus_tag="I9573_000601"
     CDS             10890..13358
                     /locus_tag="I9573_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4795235.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASRVSPKIAMAGSTTKIKRKIKREKAIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYNSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNISLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(13598..15022)
                     /locus_tag="I9573_000602"
     CDS             complement(13598..15022)
                     /locus_tag="I9573_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4795236.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMPYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANSRGR
                     FVFAVIIMALFSPFIYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMKGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(15041..15733)
                     /locus_tag="I9573_000603"
     CDS             complement(15041..15733)
                     /locus_tag="I9573_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008754589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4795237.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(15788..16564)
                     /locus_tag="I9573_000604"
     CDS             complement(15788..16564)
                     /locus_tag="I9573_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HAT4795238.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(16561..17331)
                     /locus_tag="I9573_000605"
     CDS             complement(16561..17331)
                     /locus_tag="I9573_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HAT4795239.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(17328..18032)
                     /locus_tag="I9573_000606"
     CDS             complement(17328..18032)
                     /locus_tag="I9573_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HAT4795240.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(18287..18799)
                     /locus_tag="I9573_000607"
     CDS             complement(18287..18799)
                     /locus_tag="I9573_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795241.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEETYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDISNYYLVANYI
                     RCLKKYKLID"
     gene            19125..19382
                     /locus_tag="I9573_000608"
     CDS             19125..19382
                     /locus_tag="I9573_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795242.1"
                     /translation="MILNRTELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FSNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            19525..20151
                     /locus_tag="I9573_000609"
     CDS             19525..20151
                     /locus_tag="I9573_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795243.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDNRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIEVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(20401..21180)
                     /locus_tag="I9573_000610"
     CDS             complement(20401..21180)
                     /locus_tag="I9573_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HAT4795244.1"
                     /translation="MNSKIYYFSGTGNSLVVSKILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIIKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPSDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(21248..21709)
                     /locus_tag="I9573_000611"
     CDS             complement(21248..21709)
                     /locus_tag="I9573_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT4795245.1"
                     /translation="MGGAGDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(21685..22362)
                     /locus_tag="I9573_000612"
     CDS             complement(21685..22362)
                     /locus_tag="I9573_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur flavoprotein"
                     /protein_id="HAT4795246.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            22595..22981
                     /locus_tag="I9573_000613"
     CDS             22595..22981
                     /locus_tag="I9573_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT4795247.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            22984..24783
                     /gene="blaR1"
                     /locus_tag="I9573_000614"
     CDS             22984..24783
                     /gene="blaR1"
                     /locus_tag="I9573_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HAT4795248.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVIILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSIIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLKQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(25138..27012)
                     /locus_tag="I9573_000615"
     CDS             complement(25138..27012)
                     /locus_tag="I9573_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAT4795249.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(27017..28681)
                     /locus_tag="I9573_000616"
     CDS             complement(27017..28681)
                     /locus_tag="I9573_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAT4795250.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDATPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSKYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKLVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            complement(28695..29510)
                     /locus_tag="I9573_000617"
     CDS             complement(28695..29510)
                     /locus_tag="I9573_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAT4795251.1"
                     /translation="MYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICTGR
                     SIGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAFSI
                     ESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDVHKICLW
                     SNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKETIC
                     FGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(29615..30379)
                     /locus_tag="I9573_000618"
     CDS             complement(29615..30379)
                     /locus_tag="I9573_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT4795252.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVVKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDTSTIIDFAKEMRMKGTYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(30376..31125)
                     /locus_tag="I9573_000619"
     CDS             complement(30376..31125)
                     /locus_tag="I9573_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT4795253.1"
                     /translation="MRLESLVNQSYNSLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKSGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(31629..32234)
                     /locus_tag="I9573_000620"
     CDS             complement(31629..32234)
                     /locus_tag="I9573_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT4795254.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMKIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            32413..32511
                     /locus_tag="I9573_000621"
     CDS             32413..32511
                     /locus_tag="I9573_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAT4795255.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFD"
BASE COUNT        11545 a         5401 c         3939 g        11716 t
ORIGIN      
        1 aaaatttaat ctattataaa attaccctaa ttgctaaact ttttaatata aattttccaa
       61 catcttacaa atataatcaa tagatataga atcctaattt acacaccaac atatatattt
      121 atttaaaata gtcccaaaat acctgtaata gctcccacta ctatcagtat aaacattatt
      181 tttattggag aaaggccttt ttttaaagca aagagtacta ataaagtaag taccaaagga
      241 attaatcctg gaaatatctt atcaagtaaa tccgtttgaa gactaaattt aaaatcacca
      301 ctagtataac ttattggtgt agataaatta ataaacctac ctacaagtcc ccccatgact
      361 aaagtaccta atatagaagc cccttctata accttattta ctattccacc ttgaagtata
      421 ctttcaacag cttttttccc atacttatat ccatttgtcc acattccaaa agaaattgaa
      481 gtcattatta tatattgagc tattacaaat aatataggac ctgcaattga tgctcctccc
      541 tcagttaaac caatacaaac agctaacact ataggagtta taactccttg agttaaagta
      601 tcaccaattc ctgctagtgg acccatcaag cctgttttga tagcattcat accatctcca
      661 cttatttcat ctgctccatt agctttttct tcttccatag ctattgttat tccatgtata
      721 acacatccac atataggttc tgtgttaaaa aatgctaaat gagtctccac ttcttgtttt
      781 aactcttcct tatctggata taatttttta agtactggca acattgaatg tgagaaagct
      841 gttgattgca atctttcata attgtaatta gattgcgcaa agaaaaacca tcttaaccat
      901 gattttatta catctttttt gcttaacgtt ttcttagata cttcattttt tatatctgac
      961 ataatatttt ccccctcaca tgtatttaag atgtagcact atcttttccg tacttaattg
     1021 aagtaattgt aagagcaagt attactccaa taacagatat tactattata tctactttaa
     1081 aataaacagc cattagtatt cctaatatga aaaagggcat agtaaacttg tttccaatca
     1141 attttaaatt catagcaatc ccaagtgctg gcaacatagc cccaattaca ttaaatacat
     1201 gtaacacatt ctcattcatt acttccaaca taccatctat cgcatttgga ccaaaataaa
     1261 caactaataa tgctggtata aaactcattg caaataacag tatctgtggt ggtactatat
     1321 ttataaatgc tactctatca acattccctt tttgaacctc tctatcagcc caatgtgcaa
     1381 aaaaagaatt tactgtcatt tttccaatcc aaagtacagt tgcaacagtt cccaatggta
     1441 cagctattgc cattgcagta cttacatcta gcttagatgc tatagctaaa gcagtaccta
     1501 catatcctgc aaaagctggg tcccccatct gagctccccc tgctgatata aaacctagat
     1561 atatcatatt tatacctgct cctataattg ctccttgaac agggtctcct aatataattc
     1621 ccgttacaaa tccagccaat agaggtctat tccatgtaaa aaatccaaca taggtcaacc
     1681 acggactcca agaaagataa taaaaaattg ctattaatac tgcttgaatt agtgaaatgt
     1741 gcatattctt cctccctatt ttcttttaaa gatttacaag atagtctttt ttataataat
     1801 gttcctacat caattttctt tgcatctggt actatttgta taaagcattt aacatctttt
     1861 gatattagtt ctctaaatat ttctttttct tcatctgaca cagatatatt tttataaaaa
     1921 ctttttctac caagccctgc tcccatacct ccaacattca attcctttaa atctacccca
     1981 ctatttacta aacctaagac agttttaggg tatttgacca atactattaa atgttcatct
     2041 ccattatgtg attttaaaac ttcagcacct gattctacat catgtacttc aaccttaaca
     2101 gttgatggag ctgccatttg taaaacttga actataaatg ggtcctttac tactaaatca
     2161 tccacgatta ctatcctttt tgccgatgta atcttacacc aagcagtaat aacttgccca
     2221 tgtattaatc tatcatcaat tcttgttaat gctatattac tcataattat tcctccagat
     2281 ttttaattta ttttacaact ttaaaacagt tttaacatct ttgattcctg atatcccaac
     2341 ttcctcacat aaattcttca agtcttttaa cttcatagta tctctgttta aaaatagctc
     2401 taaaagcata ggtaaattta cacctgtcaa acattctaac tcacatacct tagcaaattt
     2461 atttattata atagcagatg tattaaatgg agtcccgcca taaaaatcaa gcaatactaa
     2521 aaccccttca tccttatatt tctcaattct atcttctaac cttctactaa actcatctat
     2581 gctgtcacca tggtttaatc ctaaagtttc tacattttcc tgtttgccta ctattaattc
     2641 agcactttct aacattgctt tagccatatt tccatgtgtg gctataatta tccctgtcat
     2701 atgaatcata cctttcttaa ccttgtttat atggattatt atttagtact ctaatctaat
     2761 tatctatatt aatcattatc ttaccaaaga aaatatccct tttgtatact ttttcaaaat
     2821 aagctggtac ttcttctaaa tttattctat gtgttactaa tttttcaaca ttcatatcac
     2881 ccttttgcat atgatacaat ccagcactcc attcttttcc tgggaatgga aaagagtttc
     2941 caaaccaagt tcccttgaca gttaattcac ttcttactat cttttcaaat tgttctctag
     3001 tcaaagctac atccccatat ggtacacctg catacaatac tgtccctccc tttttagcta
     3061 acaacaatac ttgcccacat gtcattggag tcccagccga ttcaataaca atatctgcac
     3121 catctccatc agttaacctt tttatttctt ctacaatatt cttttctttt gcatttatac
     3181 aaatatctgc ccctaactct ttcgccaagt ctaacttctc atcaaataca tctacagcta
     3241 ttatttttgt agagccaaat atctttgccc attgtattga aaataatcct attgtacctg
     3301 taccaagtac tacaacagta tctccaactt tagcttctga tctaaaaaga ccatgccctg
     3361 ctatacatac tggttctagc gcagcggcag tctcataact tatctcatct ggtattttta
     3421 ttaaattcct ctctttcacc tttgtatact ctgcaaaaca acctccaagt tctttatttc
     3481 ctattattgc tacattatta catcttgaat acaatccttt tttacattca tcacattcaa
     3541 aacatggcat agctggacaa acagcaactc tatctcctat tttaaaactc cttacttctt
     3601 taccaacctg tgccacttct ccagaaaatt catgccctaa aatttctcca accatatgtg
     3661 gacctgtctt tgaatatttt gatatatcag aaccacatat tccagctact tttactttaa
     3721 ttattacatc atctttttct aatatttttg gtacatccac atcctccact cttgtgtctc
     3781 ttataccata gaatctaact gacttcacta ttaaccaccc ctacttttta tttgagaata
     3841 tatccctatt gtttatatgt taaatatcta aagcaacttt catgccaata tatgtaagca
     3901 ttgcattcat gcattttata aaattatcat taaatgtgtg tcaaagtgtg tcaaaagttt
     3961 cttatattaa aacttatgtg tgtcaatata aacgacacta acattttata ttagttctct
     4021 tattataagt tttataaaat gtactttttt tgttattttt ataaaattgt tcggaaattt
     4081 cccttctatt tgtgatataa tattaaatat aaggtatcaa tataaatttt catgcgaata
     4141 tcagaacttt tttgacacta atgccttatt caatataata atttaacttt ttggaggttg
     4201 tttatgaaaa aagtgtatca aggaaagact aaagatgttt ttgaattaga taatggaaat
     4261 tacttactta aatttaaaga cgatgtaact ggtaaagatg gagtatttga cccaggcgaa
     4321 aatagtgtag gtctaagtat agatggaatt ggtagagcta atttagagac atctgttaag
     4381 ttctttgaaa ttctaaacaa tgctggaatt aaaactcatt atgtaagtgc taatttagaa
     4441 gaagcaacta tggaagttct accagcaaaa gtttttggta atggattaga agttatttgt
     4501 cgttatagag ctgttggtag tttcttacgt cgttatggtt catgtgttga agaaggtgct
     4561 aaactagatt gctatgtaga agcaacttta aaagatgatg accgttgtga ccctcttata
     4621 acatctgaag gtttagaagc tttaaacatt atgactcaag ctcaatttga ttcaatgaaa
     4681 agcatgactc aaaaaatttc taatatagtt cgtgatacta tagctgaaaa aggtcttgaa
     4741 ctttatgata tcaaatttga atttggattc tacaatgatg aagtaatctt aatagatgaa
     4801 atagcatctg gaaatatgcg tgtttataaa gatggtgtaa tagttgaccc tatggattta
     4861 actgctcttt tattagataa atagtatata catataattt tcaaatataa taaacttata
     4921 aataagataa gctatgaagt aaagactttt taaatgttga ttatttttaa acattttctc
     4981 gcctttaatt tcatagctta tttaaattaa tatactctaa ttagtagata atagctttaa
     5041 ccctacaatt cccaccacaa taaagcctat acatgcaatt cttgatatac taataggctc
     5101 atttaagata gccatgccta caatagctgt accgacggta ccaattccag tccatattgc
     5161 atatgaagtt ccaagtggaa gatgttttag tgctaatgac aaaaaataaa aacttacaat
     5221 cattccaaca acagtaagta tacttggagt taatttagaa aatccatgtg aattttttag
     5281 ttgaaaagcc caaaacactt caaacaaacc tgcaacaaac aaaaataacc atttcataaa
     5341 ctcatccctc ttttcaaatg tatctcttat ttttccaata aaataacctg agaaatatta
     5401 taaaatatct cccaggtttt tgtccttccg tgttcacaac aaaaactatg ttgtgggttt
     5461 tatctcggac caggccaatc attaataatt gcaaaaccct ataaaactct attaattatt
     5521 ctattttatc tataataata ctatcaacat atataaaaaa agtcaatata tgggttccat
     5581 ctataataaa aataactttt atataccttt taatattctt attcacttct ctccataccc
     5641 atttaggtca atctaagaat atctaatttt tatttaatat gttataataa cataaaaata
     5701 ttgaaaggag taatttctat gaatttctat aaaattgcag tatgtcagat gatgaccaca
     5761 gaaaataaaa ttgaaaatat caatcatgct gtagatatgg taacagaagc tgccatcaat
     5821 ggagctaaaa ttatagtttt gccagaaatg tttaactgcc catatgaaaa taaatatttt
     5881 cctaaatttg ctgaagaata tccaggtgaa acaacaacga tattgagtaa attagctgag
     5941 aaacatggaa tttatctagt ttctggtagt attcctgagt tagaatatgg aaaaatctat
     6001 aatacttgct atgtatttga taaaaatgga gctctaattg gaaaacatcg taaaatgcac
     6061 ttatttgaca ttgaagttac aggaaaagtt agttttaaag aatcagatac tcttacagct
     6121 ggaaatgatg tcactgtaat agataccgaa tatggaaaag ttggtatagc tatttgctat
     6181 gatattcgtt tccctgagtt atcacgttta atggctctta aaggtgttga aatcgttatc
     6241 ttacctgctg catttaatat gactactggt cccgcccatt gggagttatc tataagaatg
     6301 cgtgctttag ataatcagat attctatgtt ggtgctgcac ctgctagaaa tatgaatgct
     6361 tcttatatag cttttggaaa ttcaagaatt tcagaccctt ggggaagaat aattgctcaa
     6421 gctgatgaaa aagaatgtat tatttatgct gacattgata gagatttaat tcctgacata
     6481 agacaacagt taccacttct taagcacaga agaactgatt tatatgaact taatactttg
     6541 aaataaaaca gaacataacc ccataaatca taaattaagt gttaaaaaaa tttatggggt
     6601 tctcattaat ttgatttaaa attaatttaa taaaaataat gctaaaatta atttaattaa
     6661 ataatactaa taattactaa tgattatgct aattgtcagc atttactaat ttaatcacta
     6721 ttagttattt cttttattaa atatctttac tttttttact tattatgtct tctagcttta
     6781 catgtggatt atggtatttc tttctatttt tagtcacatc tttctattga attgcttgct
     6841 taaaacacca atgcacacat gccagataat gttcacaatt atgtaaaaat attgctctac
     6901 cttcttcaat gttgatatta tctctcctag ttaatattat ctttcctaaa atttctttta
     6961 attaaaaaac aacattagtt gatatatcaa gtatatttta agcaactgaa aaaataataa
     7021 taattgatat atcaactaat taatgtagat taattcattt ttataattta tatacttttt
     7081 aattaatcta cctttttaat taaaatcctt cctttctatt tatccatttt caacttaaat
     7141 tataagattt catcagctgt gtttttgcat tccaataaat attaaatatc aaatatatat
     7201 tctattaaaa tttctaataa tttcatcctt gatttattta atatataaaa atatactaac
     7261 tttaatgatt aagttttgaa atataactaa gaatttgaga atttattgag atttttatac
     7321 taaaatataa atactataca aacttaattt agaagggagc attcttatga acaataaaat
     7381 actcgtcgta gatgatgagg aaggtataat aacactctta aaagattact ttgaaatgaa
     7441 tggttatgag gtttacactg ctcaaagtgg aaaagactca ttaaaaaaaa tatccaaaaa
     7501 tccagatatc attctacttg atataaacat gcctgaaata gatggaattg aagtttgtaa
     7561 tagaattaga gattatgtat catgccctat tctatttctt acagccagaa ttgaaaatac
     7621 tgacaagata aatggttttc gtgctggtgc tgatgattat attgtaaaac cttttgattt
     7681 agacgaactt ggagctagag tatctgcaca tttaagacgt gaacaaagga agtcaaatca
     7741 aactaaaatt aaattttata atgatttaac aatagattac gaaaaaaggt taatttgtat
     7801 atataataat cagatttcac tttcaaaaaa ggaatttgat atagtagagc ttctctctat
     7861 gaatcctgga caggtatttg ataaagaaaa aatatatgat agagtctggg gatacaatag
     7921 caatggaaat agtgatgtta taatggaaca cattagaaaa atacgaatca aactatcaaa
     7981 acatactctt gaaaattata tagaaacagt ctggggagtt ggttataaat ggataaaata
     8041 agtaatatgt cattaaaaaa agctttcttt gttcttactt tatcttcact aattatagca
     8101 tcagtactta ctactttcgc atacatttta cttaataatc tatataattc tatacaagat
     8161 aagtatttaa aaacacctat aaatacaggt attataacca tagttcaaaa caatgtgaac
     8221 caatttagtg attatgataa aacattactt aatataatca acatacttca gcttgtactt
     8281 ccacttatat tttttatagg acttttatta ttagctgata taatttttta caaggtaaaa
     8341 ttaaaaacac ccatagaaat cctcaataaa ggtgcaatgg aaatatccaa caacaattta
     8401 gatttttgct tagaatataa taataatgat gaattaggta atctatgtaa tgcttttgaa
     8461 aaaatgaggt ctgaactaaa taaaaacaac ataaatatgt ggactatgat taatgataga
     8521 aaacaactta atgctgcttt ttctcatgat ttaagaaatc ccctaacagt tctaaaaggt
     8581 tactcaaact atttaacaaa atacatacca acaggcaaac ttagtgatga gaagatttta
     8641 tcaaccacac aacttatgtc tgagcatatt aacagaattg aatactatgt agaaaatatg
     8701 agtaatgctc aacgattaga agatttagtt gtttcaaaat ctatgtcgaa tataaacaag
     8761 tttatagaaa acttagatga aaatatatct atcattgcta aacaagaagg aaaatctttt
     8821 acacttaaaa gtcaaataaa taatataaat ctattttttg atgaaaatat cattcttaga
     8881 gtcgtcgaaa atataatatc gaatgctttt agatatgcta gaaataatgt atccatactt
     8941 atatatcttg aacaagaact ccttaccttt gtaattgaag atgatggtat aggatttagt
     9001 aaggaatctc taaaattagc tttaagacct ttctatagag ataagacttt aaatgacagc
     9061 aatccacact ttggtatggg tcttcatatc tcaaaaatat tatgtgaaaa acatggtggc
     9121 tctatttcta ttgaaaataa ttctactaac agtgctaaaa ttactgctaa attctcaaca
     9181 agaaattaaa atgattttgt aagctttcat tgttcttata taagctttgt tagtttagaa
     9241 tttataaaat gtatttcaat ttacaaccaa tatatatact attttgtaga agttgtaaga
     9301 gttgcttcaa ttataatatt ttggcaaaag gcatttgaaa caataataga aatatatatt
     9361 taaatatgta gaatagtatg aaactagaat tttggaggtg attatattaa aagttttgct
     9421 aataatatta cattctctcg tattgtgttt gcattgctaa tgcttagttc tacattctgt
     9481 tcactgcctt tctggatatg ttatttttgg tgtggaatta gtgatatact tgatggtctt
     9541 attgcccgaa agttgagaca acaaagtgga ataggtgcaa agttagatag tattgctgat
     9601 ttcatttttg cggttacaat tttggttact gtcggaaaaa atattgctct tcccatgtat
     9661 ctatggtggt gtattgttgc aatagcatta ttacggttga ttagttacag tattggtttt
     9721 tttaagtatc gcacattttc atcgcttcat actttgatga ataaaatcac tggaatttct
     9781 atctttgctt ttccattact ctatatttgc tttggtcttg atgttgcagg aattatgata
     9841 tgtatcgtgg cttttctttc ttcttttgaa gaaatagcaa ttatgataaa atcaaaagaa
     9901 ttaaatcgtg atataaaaag tatatttatg tgacaactta gaatttgtga caaaaataat
     9961 ttatataata atgaggaagt aaaacataaa aaattatcca caagtttatg tgtaaaatag
    10021 ttttaaagca atagagttgt attgtaaagc ctttagtgca taaattacat tttaaattaa
    10081 aaatgatact gaaactgctt atcagcactg cgaattatcc gtaaatagtg aagacttttt
    10141 agatttaagc taaaagttgg gaaaaaattg agttttaaaa cttacactct tcttatatag
    10201 aaaaggagag tgttttttat gattataaaa gcaaaacaat tatcaaaaat atatggttct
    10261 aataacaata aagttatagc acttaataat gtaaatttaa aaataaactc aggtgaattt
    10321 gtttctgtaa ttggaccttc tggaagtgga aaaagcacac ttttacatat tttaagtgga
    10381 ttagataatc ctacctctgg acaagtgtta cttgatgata aagatatata taaacacact
    10441 gaaaaagaac tatctgcact tagaagaaaa tcctttggat tcgtctttca acaatttaat
    10501 cttttacctg ttcttacagc atctgaaaat atttcaatgc ctgtacttct agataaaaaa
    10561 caacctgata aagactattt aaatgaaatt tcatcattac ttggaattgc agatagactc
    10621 aatcatcttc cccatgaact ttcaggcggt cagcaacaaa gagttgcaat cgctagagca
    10681 cttattgcaa aaccagatat aatatttgct gatgagccta caggaaactt ggacagtaaa
    10741 agtggcagtg aagttatgaa tcttcttata aaaacttcaa aacagtttgg aaagacttta
    10801 gtggtaatta ctcatgatga tagaattgca aaacttgctg atagaaaaat ttcaattatt
    10861 gatggtgttc ttatggaggt gaaatagaaa tgaagaatta cctttcactt tcaataaaag
    10921 aattaaaaag ccagaaactt atgactacat tcatcattat agcaatagta ctatcaacta
    10981 ttatgacaac tgtggttgga cagtcaattg gtatcctaca aaatttaaga attgaacaag
    11041 cccgttcttt taatggtgat agacatgtat cctttcacca acttactaaa aatcaagttg
    11101 atgacataaa gaaagatgat agagtttatc aagctggtac ttcaataact attggcagta
    11161 gtaaaataaa ggattctggt atctctgtac ttgtaaaaga gtatgataaa acaggacttt
    11221 ctaactaccc aaaattaatg aaattaaaat ctggacattt gcctaaagat aaaaacgaaa
    11281 tagccttaga tgaaaacact ctaaagctta tgggtataaa accaagacta ggagtgacta
    11341 tacctatgaa tttagatatt tcacttttaa atgataccat accaccatat aattacacag
    11401 ctaactttaa attgagtggc attttagagg atgattatac aggatatgta agtggtatag
    11461 taaatggtat tgtaggtaaa ggtacatcag aaaatctatt acctaagaga tatattttat
    11521 cctctttgga ttttaaaata aaacagcaag aaaagtttca agaaatagta aatcaattag
    11581 ctaaaaaaat aaatctttct cacaattcaa ttcaatataa ttggatttac ttaaatgctc
    11641 ttaagataca gtttgaaaaa gatgaaaata gttctaatag tgatggtata tctatgatta
    11701 tattggtatc tttatttgtt gccttattgg ttttactggc ttctggactt gtaatctata
    11761 atattttaaa aatctctgtt acaaagaaaa ttaaagagta tggatgttta agagcaatag
    11821 gagctgaacc aaaccagata tataagatag ttattttaca aatcttaatt ttatgtactg
    11881 ttgccatacc tattggagct gtaattggaa ttatctcttc taaaggcata actggaatgg
    11941 tcactaatat cttaaatcca gatatacttc tagcaaatga taataaagaa attactgagc
    12001 ttatacataa aaatacaaca gcatatatgt tcccattagt attaagcact aatgtatctt
    12061 tgattttttc atttatttca gcattaccat cagcaatata tgcttcacgt gtctctccca
    12121 aaattgctat ggctggtagt actaccaaaa tcaaaagaaa aattaaaaga gaaaaagcaa
    12181 taaaaaactt tgaaagacat ctagcatggc taaacttaaa aagaaataaa ggaagaacta
    12241 taataactat actatcatta tttatgagta taacagtatt cgttgcattg agtgagttct
    12301 ctaatgtatt agatgtatct aggtcagtaa gcaatttaaa agaaggagat ttttccttaa
    12361 caaatgaaat atctggtttt gataaatcaa cactagataa aatagataaa atgaaaaacg
    12421 tcaatcgcac ttcctttata aagtactctg aatacaaaca gggtgaaata gatacagata
    12481 taaattttga aaatagtggt gagatgttaa aaatcattgg aattgacgag cagactttaa
    12541 aagacttaat gccttctatt acagattcta tactagaaga ctttaaaaat ggttctatct
    12601 gttttataaa aaatccgatt gctatttcta caccaggtgt aaaaactaga catacaaatt
    12661 taaaacctaa agatagtatc acaatcaaca aaaaacaact agatatatat agtacagtag
    12721 ataaaatgtt ctttttacaa ggtaatggct ggataaatgg tgttgatgtt attgtatata
    12781 attctgttta taacaccttg acaaataaaa acaagttcaa ccagataaat atttatgcta
    12841 aagataaatc aaaattagaa caaataagat tgtctattga acagatttgt gaaaataatc
    12901 ctggctctca ttggatatca tatattgaat cagataagca acttaaagag agttttaaac
    12961 aaatagaatt tctagcatgg gcagttatat tatttgtggg attgattggt actttaaaca
    13021 tcattaacac aactcataca aatataaata ctaggactaa tgagattgga gttaaaagag
    13081 ctattggtat gagtaatatt agcttatata aaatgttttt atgggaaggt gtttattatg
    13141 gaatttttgc tgctatattt ggttcaattg ctgggtatgc cagtgctatt attataaaca
    13201 tggcaactat agaaaagctt gatttcacta atataccaat tacttcaatt ttacaagcta
    13261 ctattatttc tgtacttgca tgtattatag ctactcttat tccgttaaga aaagttaaaa
    13321 agatgaatat aattgactgt atagatatca atttataact attttacaga taataatata
    13381 aattaaatta tcaatgactg tctctatttt ctttcaaatc taactaataa atctttttaa
    13441 ctataatata agctttaaaa aaggcctgtc tcaaaataga gataagcctt ttttcattgt
    13501 cggaataatg aaaatatatt taacttcaca aaaaaagagt gcctcacgaa ttaaaaattt
    13561 cgctttgaga cactcccctt taatttaaaa tatatagtta aatgctatgc caaaatttca
    13621 cacatgctcc acctgatttt gaattttcta ttttaacaga acctccaagc tgttccacta
    13681 attgtttgac aatgtaaagc ccaagaccag aatgtccacc ttttgtctgc ctagcttcat
    13741 ctcctcgata aaacttatca agagcctttt ttaaatcttt agagctaaac cctctaccag
    13801 aatcacaaat ctcataagaa atacaatttt tttcatcctt tacagtaata ctaatattac
    13861 cacctgatgg agtgtattgc agactgttgg atatgatatt atcaaaaatt cgtgtcaacc
    13921 tatccacgtc gatttgaatt ggggattgta tattcccctt cattttcaat ataagttcaa
    13981 cttccttttg atgagcctgt aattcatagt cttgcacctt ttgcctcaaa aattcaggta
    14041 aatttatagg aacaagattt aattgtatat ttgacttttc taagtcagac gtatattgca
    14101 tcttttggac aagagcagca cttttttctg tattttctct tatcactgta agatacctat
    14161 gaagtttttc attgtcatct gtattattac caataagtgc atctgtatat cccaaaataa
    14221 tagaaagagg agattttaaa tcatgtgcta gagcttccac catttcaaca cgttcctgct
    14281 ccatcttcca ctgcgcagat aaagaacctt ttaattcgtc tttcatttca gaaaaggcac
    14341 tacaaagttt accgagttca ttatctgaat aatagtcgat ttcaaaatca aggtcctttt
    14401 cttttatctt cttggacgca tcaatcaata gatgtaatgg ttgattgata ttctttacaa
    14461 agcgcttaga aagccatcta gtaaaaccca cgatatatat aaatggagaa aacagagcca
    14521 ttataataac tgcaaataca aatctccctc tactatttgc aaaggttatt ttgacttgat
    14581 agaatagaat aacagctccc tcaatctttc cattatcccc tttgattgga acagtatgga
    14641 tataaccacc tttacgaaca gttttattga taaaattatt aaataattct tcttttgttt
    14701 taaatagttt tttggggtta gtaccatata gaatattgcc attattatcc acaacttgat
    14761 atggcatatc atccccacga atcgttcttt ttaaaccttc ttcattcgat tgtgataaca
    14821 aagttatgtt tttttcattg atgtattttt caattctagg aacttgtcgc tcataatagt
    14881 ttggtggata gatatcctta tttaaggaat gaatatacaa atagagagca aatacataag
    14941 taatcactgt ggcaactata ctggctatta taataaaaat aaaagtatgt ctaaattggg
    15001 taataaggga ttgatttttc atttaataac ctctctctct ttacatttta ttccacttat
    15061 acccaatacc ccaaactgtg gaaatatatt ctttttctgg tgtcacagca gaaaacttag
    15121 ctcgtatttt ttttactcgt tcaacaacag ttgaggaatc accttcagaa tcacaccccc
    15181 atactttttc atagatttgt tctcttgaga aaacttgtcc tgcatggagt gctagtaatt
    15241 caacaatatc atactctatt tttgttaaat caatattctt accatcaatt ttaattgcac
    15301 gttccttcat atataagcat aacttcccaa aatacaaagt agatctcttg ttctcttcat
    15361 tgatatactg tgaacgcttt tccctacgca gattagcttc aatccttgcc ataagttcac
    15421 gcagtccaaa tggctttgtt acataatcat cccctccaaa ggtcaaccct ttgattttgt
    15481 cggtttctga ttgctttgca cttaaaaata taattgggca taaaacatca tctctaattg
    15541 cctgacatac ctcaaatcca ttcattttag gcatcataat atccagtata ataaggtcag
    15601 gctcctgttt tgataactct atacctgcct gaccatcata agctatcaac acttcatgtc
    15661 cttttgcctt caattcatcc tcaagtagca ttactaggtc catttcatca tcaataatca
    15721 atattttact caatataaca ccacctttct cttatgggaa ggtcagtatt aaaacaatat
    15781 tcgtctacta ataacttttc cttccctccc acattttaaa ccaaataacg cctcctatca
    15841 agaatataat caaacctatt gctactacta taagctctat actagctgtt tgcatcactt
    15901 tagagtaaaa tgcctcagtt gaaatagttc cagcttcttt aataaaataa attcctggaa
    15961 gcatacctag ttttactggc catgtccatg gaacaaatgc ccatactttt tcaccaacgc
    16021 tagttaatcc aagtattgct gccatcaaaa ttccaccgat actaatacca atagatgcac
    16081 ccatacccca tgcaaaactc acccaaagat gaatagctaa tagtggaagt gtcccaatca
    16141 ccacaagtcc tgctgctgac aggaataacc atattgttgc tccagatggc actgcaatac
    16201 tcattcctat gcaaagtatc aacgtagcaa taaatgtgca tatagttagg caaaacagaa
    16261 gaaaaagaaa ttttcccaga tataatctaa cacgagaaat acctacacat aaaaaaccat
    16321 taaaatttcc agcaagctct tcctcctgca ctataaatcc tgctaaaata cctactccaa
    16381 taggaatgat gaatacagtc catactgtaa aaaatccttc aaaagcaaat tcttgtgtac
    16441 ttccagattt agttgtgaga taaaaaacca cacataatga aaaaataata ggcatacaaa
    16501 gagtaagcca ctggacagct gtcctctttg ctcgaagcca ttctgatgat aatagcttta
    16561 tcatttgtca tccctcctat tgaaccaagc tgcagtaagc gctgttaata ctataaacac
    16621 aaccaaagaa acaacaatcc caataggaat cactgaagta ttcaaaagtg ggtctccagc
    16681 ttctaatata gtaccgttag gatgtatgtt gatgattggg cataccattc taactgccca
    16741 actccaaggt actgcaatcc aaaatggttt tgtcgcagca aggacaccaa atatcatacc
    16801 taaagctcca actcccatac ttaggaacat acctttccat gtagctagcc atagctgaag
    16861 tggaagtata gcaagagaaa caacccagca tacaatactt gcccctataa tctgtccaaa
    16921 aggaactgga ccagctttca atattaaacc agttataatt gttacgacta ttaaaactaa
    16981 cgttgaaata aaactgtaga ctgccatagc tataacttta ttaatccaaa gtgtcatagg
    17041 agacacattg tgtgtacgta aagcacgata atttccagct tttttttcct ggaaagctac
    17101 caaagtagca aacaatgcaa atccaatagg cagaaataaa aatgaccacc agttaaaaat
    17161 taatgctaaa agactttgcc acgcccaaga agttgtttga gataatggat tttgcatgag
    17221 tgttgattgc attataaatg catatgctgc aaaagacact gggaaaaaaa caataagttt
    17281 acccataaat gttcttttat actttaaaag ttctgattgt aaagcattca tctgttttgt
    17341 cctcctttaa gaataacatc tgtaaaaaat tcttctagat tttcattagg gtcaggcatt
    17401 ccttgaaata gtagtcgtcc tccatttata attcctatat tatctacaac ttgagcaacc
    17461 tctgatagaa tatggctaga aagaattaca gttatacctt tttttggaaa agaagcaatc
    17521 aattcacgca attcttgaat gccaaagggg tcaagaccat tggttggttc atctagtatt
    17581 aacaactctg ggtcactcaa aagtgcaata gctattccta acctttgttt catacccata
    17641 gaaaattgtg aagctctctt tttacctgta tttttcaaat ttactatttc caacacttca
    17701 taaattttat ctttaggtat tcccaatatt tttgtatgta ctaacaaatt ttcctctgct
    17761 gtaaggtttc catacaatgc aggggattct attaaagaac ctatttttgc aagagattct
    17821 cgtttccaag gctttctatc aaaaataata cgaccatctg ttgggcgaag aagaccaaca
    17881 agcattttaa gtgttgtgga ctttcctgct ccatttggtc ctaaaagtcc atatatacta
    17941 tttcgttcaa tttgtagaga aacattgtta actgccattt gttctccata tctttttgat
    18001 aagttttttg tttcaagaat taaatctttc atattaattt ctcccttctt gtaatacctt
    18061 aaatctatta ctaagagaat actaacatga aaaaataaca attctataac acctactaat
    18121 tttaataaaa attaaaataa aaaatagagt taccaatttt attttggcaa ctctatttaa
    18181 gtttatgttt tacagttata ttagatttaa gcttagtttc attatcacac taacttcaaa
    18241 tttactctat aatccttaaa tcaagcacta atttattaat caaacactaa tctattaatt
    18301 tatatttctt tagacatcta atatagtttg ctactaaata ataattactt atatcatatt
    18361 tatctaatcc tttaaaagaa gttatttcat cattaagctc atcactacct tctacaaaaa
    18421 cacaacctgt ttcaaattcc atacctacaa taatcttaca gccactataa gtttcctctt
    18481 gagtatcgta caattttttt gcatttcctt catttggaat tgtaaataca ataggtttta
    18541 attctttatc actggaaatc ttttgtgaaa aaggcttgtt taacttaata ttattgataa
    18601 ctacatgttt aaattcctct gaagtttctt ctactattac atattttcgc tttaaaaact
    18661 ctattatttc taaaacagac ttagaattta cttttaaaat acttttatat ttttcatata
    18721 aagatttcca ctgttcaatt ttttcctgtg gtacttcttg gatatgtgta cttatttttt
    18781 cttgagatat ttttttcaat taaactctcc tttcaatact taaatatcct tataaatctc
    18841 acatgactaa agttacaagg ttcctactta atagaatttt gcatacttaa atatgtctta
    18901 ttaattctct acaggatatc tccgtagtcc ctacgattct tacataagtt acctatttta
    18961 aattgttatt agttagatta taaaaattat ttattttatc actaatataa tactcttaaa
    19021 aactttacaa atcaatttaa atttgctacc ttttatttat aacaacttat agtacatttc
    19081 tcctatgctt ttcaatatat tacttttaaa aggaggtttt ccttatgatt ttaaatagaa
    19141 ctgagctttt ccgaggatat tctttaaata gatttaacga gatatgcaat atattatcag
    19201 taaataaaat taaatataag tataaagtga aaaataatgc ttactctaaa aaaccttttt
    19261 ctaatgaaat aacacttggc aatttaggtc aaaaggaaga tttctcttat gagtacttaa
    19321 tttatgttca taaagacgat tatgaacagg cagagtctct aataaattct aagttgcgat
    19381 agattattta taaaacatga cattctgttg tcatgtttta tatgttatta tactttcaaa
    19441 tatgctacta tattttcaaa attgtgtgct ttaaaactag ctttttatag taatttaaag
    19501 cttaaaacta aggagactcg ttatatgaaa ataaatataa aatcaaagtt aagtgaattt
    19561 attaaacaga acaatctttt tgatgacaac agaataatat cctaccttcc taatataaca
    19621 atagaaacag acaaaataaa aactataatg attaatgagg ttgtcccaac taacaccaat
    19681 gacgattttt atagtgtaga taaagatgct gattatttaa aaactactat tcctttattt
    19741 gacagtgcag gaatagaagt atctaacata aatgatatcc tagatatggg tatctatata
    19801 actaatgcag taaaacttcc taaatcagaa tatacaataa ctcgtgatac aattaaatta
    19861 catatgccta ttttagaaga agaaattaaa ttatttaaaa acctagaggt agtaatgtta
    19921 atgggagatg ttgcaaaaaa atcttttaat atgattacta aaaagcatat aaaaaagaat
    19981 gtcatccctt ccatttcaac atataaactt cgtaataatg aactttatta tgataattta
    20041 agagtatttc catcatatat tatgactggt ggaaacatac tgattgaaaa atcaaaattt
    20101 gaaatggcat cagaagatat aaaaaaaatg ttcgagatta taaacggttg atattacaaa
    20161 ataataaaaa ctaatttagc caaataataa gtgcctcaaa aacttctatt tatgaggcac
    20221 ttttatttta tattagtgta actatatact attaacataa tttattattt atattgttct
    20281 ttttaatctc tattccattt agtctaggta acattactct ttacaagatt actttatatt
    20341 tcttttatat ctctaacgtt tttatttaat aaaacaatta ttaatacaag tcaaatatat
    20401 ctatttatct aaaaatatat cttttacagt aatatctgga tggtgatact tcttcctatt
    20461 cgttaaaaaa gattttttta cattaatagc ctcttttgga caccagtgaa aacaagctag
    20521 gcatctttca cactcattac cccactttgg ctttttatca actatactta tattctctac
    20581 aggacaaacc cttttacatg tcccacatcc attacattta ttttctgcaa aaaatttagt
    20641 agttgcaatt tgcatattct tacctactaa acgaagtaca acactatcta tatgtgactt
    20701 taaattgttt tttccttcta tatttccaat ctccaagtta ttgatacatt ttgcaatctc
    20761 agctacccta ttagtctgat ttttcaatct ttcttcttca acatctgaag gtgttatcaa
    20821 agcatttcca ggcatattga ttaaaaaacc agcagataat tttttccccc tttttttaag
    20881 taacttatct atagtaaata aactatgtcc agctacccca ttacaagtgg caatggcata
    20941 tatatgagta ttaatattaa aatttaattt ctctacaaaa gactttatta tatcaggaac
    21001 atcagcaaaa taaacaggaa aaacaattac caatatatct tcatttatgt ctatattatt
    21061 ttcttttcta tgtatagcta aaggtataac ttctttactt ccatttaacg tttcttttaa
    21121 aatctttgaa actactagcg aatttcctgt tccagaaaaa taataaatct tactattcat
    21181 tcttatccct ctttctatat ttctacatca tcaatagtta aaaagtctct taaaatagga
    21241 tttatattta ctttccaatt ctatccaaaa tatcagtaaa caaaggcaat tcttcatcag
    21301 agatagattt aaatgtctct gttgcatata tacttgcaat ttcatttaat ggttcttcta
    21361 attctctacc taaatctgtt aggaatattt gaaacttacg tctatcttta acatctactt
    21421 tacggacaat taatccctta tttttcataa cgtctaaaat tcttgttgta ttagatttat
    21481 ccttacatga tttttcacat aaatctatct gagaaattcc atcttgctcc catagaaacc
    21541 ttaataacgc ataatggtca gttgttatat catatccatt ttgtttaaac atctttttta
    21601 aatcatcttt tacttttaac gaagtactta ctaacttagt tgtagcttca tatagtttct
    21661 caaaatcttt cattttacct ccatttaaat aatcccctgc tcctcccatc tatcaattat
    21721 tcccttatat tgctcaatat ctttaaaagc cattggcttt aaaacagcat ttatagcaat
    21781 aaaattatag atttgagaaa gcacaggtgg atttttcttt tcctctatgc aataaataaa
    21841 ttttttagat accttttgta tctttgattc tactttttca gttataggat tttgattaaa
    21901 tccagttttt atagataaac taccacaaac ataacctccc gaatctaaga ccatggattt
    21961 tataaaagaa attgcttttg aaccacgtgc attttcatat gtagtaactg ctatacatgg
    22021 cttacgatac aataatcttt caagcatcat atgtgctcta tctgtaaaca ccttgaaaag
    22081 tccagttaca ctacttccat aagttgggct accaaatata acaccatcac tcttagtaat
    22141 tatatcatga atatactcta ctttatcatt ttgattgata cacttgccca ttttataaca
    22201 atttaaacaa ccaatacaat atgatggatt tacctcactt aaatcaaaat aattaatttt
    22261 tacatctggt attaatcttt ctatatcttt atacaagtaa gttaaaactt ttgatgtagc
    22321 tccatttttt ctagggcttc catttataac tgtaattatc atttctatat ctccttttta
    22381 gttgttatat caacaataag tatatcaact atatttttag ctgtcaatat aaaccattta
    22441 tttaaagcta tacttacaca gcttaaataa aagttttatt taactacatg tgcttattta
    22501 atcaactata aatgaattat ttttaataat tgacaaacta aattaatgat aatatacttt
    22561 atgtattaca aatgtaagta gaaggaggta tagtatgaaa aagttaccac atatttcaga
    22621 agcagaatat caagttatga aaattatttg gaaatatgct cctatcagca ctactgaagt
    22681 aattgaaaag ttggtagaaa caagtacatg gagtcctaaa acaattcaaa caatgttatt
    22741 gagattagtt aaaaaaggag ctttaacata tgaaaaaaat agcagagtat tcgtttatac
    22801 tccacttgtt aaagaagaag aatacgtagc tacagaaagt agttcttttt taaatcgttt
    22861 ttataatggt actcttaatt ctatggtact aaacttttta gaaaatgata aactatctga
    22921 agatgatatt gaggaactga gagaaatctt aaataaaagg acgactaagg gggataaata
    22981 aatatgtatg ccccattctt tatacgattt ttactcagta cattaacctt atcaatatta
    23041 attgttataa ttttacttgc caaaaaagta tttaaaaaac atatttctgc aaagtaccaa
    23101 tacaatattg gatttatatt gcttataatg ttaataattc catttgtccc tcttaaatat
    23161 attaatcttg gaaatgtatt taactattta tatactttta atcaacttgg aagcagtact
    23221 aataggactt ctattataaa aaatagtcca aattctataa ttaataactc aaatgcatta
    23281 aacgattttt cagtatccct aaaccaagtt aattttgata gtttaaatat tttattacta
    23341 atatcatgga taattggaat tttaatcatg atagctatta ctattcattg caatattaaa
    23401 ataaaaaata tgaaaaaatc aatacaaatt ttaagagatg aggagatagt atttatattt
    23461 gaaaagtgta aaaaagatat tggtataaat aaggatttta tactatgcaa atcacctatt
    23521 ataaatacac ctattacatt tggatacttt aaaccacaca ttatattacc agataagtct
    23581 atatctaaat tatctttaaa agatataaaa tatatcttac ttcatgaatt acaacacttt
    23641 aaaaataaag atatactgat aaattatata atgtgcttcc ttcaaatatt atattggttt
    23701 aatcctcttg tatggtatgc atttaaagaa atgcgtatag accgtgaaat tgcatgtgat
    23761 atttcagttt taaaaaagct tgataaagat agtcatatag aatatggaca aactctaata
    23821 aactttgtgg ataatatttc aagaccatca aatcttacac ttacatcaga tattggtggg
    23881 tcaaaaaaac aaataaaaaa gcgactattg aagataatgg attttcataa tgaatcaaat
    23941 acgttaaaat tcaaaagcat tttaattttt acattgatag ctttaattgt atttggaaat
    24001 attcccaaac tatctattat ggcagatatc aacgaaaaat atagacctac aaatgaacaa
    24061 actgtgtacg aggacttaag tcagtatttt aaaggttttg atggaagttt tgttatgtat
    24121 gatttaaaat ccaagcaata tcatatttac aataaagaaa aaagtaaaac aagaatttct
    24181 ccaaattcta cttataagat ttatagtgcc ttattgggat tggaaaatgg gataataaca
    24241 agaaataatt cacatatccc ttgggatgga aagcatcatt ctaatagttc ttggaatgaa
    24301 gaccaagact tattttctgc tatggaaaac tccgtaaact ggtactttca gactctagac
    24361 aaaaaaacag ggctaaaaaa catacaaact tatcttaagc aaattggata tggcaattat
    24421 gatgtttcag gtggtatctc aaacttttgg ctagaatctt cattaaaaat atcacctatt
    24481 gaacaggtag aattacttga aagtttttat acaaataagt ttaaatttga agataaaaat
    24541 atagaaacag taaaagattc attgttaata tcaaaaaagg atggggtatc actttttggt
    24601 aaaacaggca ctgctaatat aaatggaaaa gatgtaaatg gttggtttat aggttatgtg
    24661 gaaaaaaatg aaaatactta tttctttgct acaaatattc aaagcaaaga ttattctaat
    24721 ggtagttcag catctaagat aacattatcc atattaaatg acaaaaaaat ctataatcct
    24781 taatatattt actgtacatt ctaaaaaatt cacataattt tcatattaat tatcgattaa
    24841 attcatatac aaggcttatt atataaacat agaagatagt aaaactagct taaaaacata
    24901 atataatacc ctatacaaat agtggcaact ctccctcaca tcctcaattg ttgccactat
    24961 ttaatttttt gtatgaagtt aaatatattt tcatcatttc aacaatttgg gtgcagtaca
    25021 atctctattt tgagacaacc tctttaattg ttttagagtt tatgaattat ttctcatact
    25081 ttaaaaagat tgaattactc ttgataatct tcttttgtag tcattacata tcacctacta
    25141 ctcaacacat attctttgac ctatttttac atccttgttt atatccaaaa tatttacctt
    25201 cttaccatct ggaaacaata ccattgtcaa agtagacttt cctaattcat gtattctctt
    25261 taaatcaaca ttaacaagaa catctccctg tttaaccata tctccttgtt taacagcaat
    25321 atcaaagcca tctccattta atgcaactgt atcaattcca acatgaatta atacttctcc
    25381 attagttcct ttaatcccaa atgcatgtcc acttgaaaat actgtttcta atttacctga
    25441 aataggagaa acaatttttc catctataat atcaatagca aaaccatcac ctaacatttt
    25501 tttagaaaat gtttcatctg gtacattttc taatgcaaca attttcccct tagctggaga
    25561 cacaaagttt tcattatttt cattaaacac taattcttcg tcttcatctt catttatagc
    25621 ttctttaggc actccaaata aacatgttac aataaagcag aatgcaacca caaatattga
    25681 tactaataag tatgtgatta aattatatcc atcatagata tacattaaat aagaaggtaa
    25741 aactgctaat ccatatgaat tagattgaat atgtaaaatt gataatattg ctgctcctaa
    25801 acctgaagat gcacacataa taatcaaagg ctttaaatta tatcgaagct gaatcccaaa
    25861 taaaacaggc tcacttatac caaaaagttg agatagcatt gctccaactg cagtttgttt
    25921 aacttgatta tttttagatt ttttcataaa tgctagacat gttccaatat tcgcaaatcc
    25981 atacattgca caaagagtaa taagtgcatt aaatcctgta tttgctaata gtgatgtttc
    26041 aatcattaca taagtatgat gtaagccagt aataacagct aatggatatg taaatccaat
    26101 aataaatcct ccaattccta aaggtaaacc aatcaataaa ctgatgatat caaccatttt
    26161 taattctatt gtatgcatta taggccctac acctaacaaa ataactaaca ttgttattag
    26221 cataacaaag aatggtgtga aaataaggtc taatacatta ggcatatgtt tacggaacca
    26281 tttttcaaga ttagctccaa taaatgctgt aacaatagct gttaaaacac ttccttgaca
    26341 gcctacaata ggaataaatc caaatgccat aattgcttca acttcatttg atgggtcagc
    26401 aacagaatat gcattaggaa gtattggaga aactaacata agtccaataa caagtccaat
    26461 aacaggtgtt cctccaaata ccttaaatgc tgaccataca ataattgctg gtaaaaaagc
    26521 aaatgctgtt tctgtaagaa ccgaaacaag tgtctgaata tacaatggaa tatttgcaga
    26581 agacattcca aataatccaa gtacattatc attaaacaaa caacccttta atcccaagaa
    26641 taatcctgtt gccgcaataa ctggaataat aggaacaaat atatctgcta gagttctcat
    26701 aactttctta aatccagttt cattattttt aactaattca tcttgttctt tttttgataa
    26761 agttttcaat cccatttttt caacttctgc ataaacttta ttgactattc ccgttcctag
    26821 aataatttga tactgtccag aagtaaagaa aacaccttta acctcattaa ccttttccac
    26881 tttcttatca ttaataattt ctctatcttt aaccatcacc cttaatcttg tggcacaatg
    26941 tgtaatagaa ataatattat tttcaccaat aatttcaata agctcatttg ctattttatt
    27001 atatttattc atttttctac ctcactttca ataatactgc ttgaaatggt ttcaaactca
    27061 ctgttttatt tagtagattt atatcatcta aattttggaa tacaatatca tatttttctt
    27121 ctgtttttat ttctacaagt ttatcagaaa aattataata acaatcaata actaccaaat
    27181 ctaattgcct tctatacgca ataacattgt cactttctat atcaataggt tctattgaac
    27241 cataaatcaa acaatcagaa tacttactat cttgtctcaa ctttatcatc tttttataaa
    27301 attctaaaat actttctgaa ttgttaatct gactctgtgc attaatatct ttataatttt
    27361 caataacctt taaccatggt gttgcatctg aaaaacctgc attttcttta tctgcccatg
    27421 gaaatggcgt tcttgcatta tctcggcttc ttaaattcac aaattctaac gcttcttctt
    27481 ctgagtaacc ttcatctatt gaacgatagt attggtcaat gctagataaa tcattaaaat
    27541 catttatggt atctctttta aaatttgtca ttcctaattc ctgtccctga taaataaatg
    27601 gtgttcctct taaaaagaaa tacattgctc ccaacatttt tactgcatca tcattccttt
    27661 cgtattgcaa aagatacttt gttgtagctc ttggttggtc atgattttca ataaaattag
    27721 ctgcccatcc atgttcctga acatacattt ggctatctaa aatgcatttt cttaaatctg
    27781 gaattgtcca tgaaattctt ttaaaccact cactaccaga agcaacatct aagtcagcat
    27841 gtttaaagtc aaagaccatt gaaaagaaac catcttctcc aataaaatca cctagttggt
    27901 catagctaat acctgctgct tcagcaacag tcatacaatc atatttttta aacgtatttt
    27961 cttttaattc ataaagcatt ttttctattc caggctgatt tctaactgtt tttgtacatt
    28021 taacaagacc atcaacacca tcactttcta aactttgaaa acttaaattc tttttgataa
    28081 atgtaatagc atcaactcta aatcctgcca ctcccatttg agaccaggta ttaatcattt
    28141 ggtaaagttc acttcttaat attggatttt cccaattaag gtcaggttgt tttttaccaa
    28201 aagaatgaaa ataatattcg tctctattct ctaccttttc ccaaacactt ccaccaaaaa
    28261 cagacctcca gttatttggt aaatcgtttc cttttttaaa aatataataa tcatgataag
    28321 gactttctgg attttttaat gcttcttgaa accaaggatg ttcatcactt gtatgattaa
    28381 gaactaaatc taatataatt ttgattcctc tatctttact ttctttaatt aactctttta
    28441 aatcttccat acttccaaat tcacttgcaa tatctaaata atcactaata tcatacccat
    28501 tatcgtccat aggtgattta aaaattggac ataaccacaa tgatgtaata cctaaatctt
    28561 ttaaataatc taaacgagaa ataattccct gtaaatctcc aattccatca ttattactat
    28621 cttgaaagct tcttggataa atttgataaa cgatttcttt ttgccaccat ttcttttcca
    28681 taaattactc ctttttatat gatatttctt ctttttaatt ctttataaat tccattatcg
    28741 aatatgtcct cacatataga tgtcgctata tctttaagtt gttgatgact atttttcatt
    28801 gcaattgtaa cgtctgatgc ttgaaacatg acaatatcat tttgaccatc accaaaacat
    28861 atggtttctt tttgagttac ccctaatctt tcttgtaatc tcttaattgc tttgccctta
    28921 tgaaaatctt tttgaataat ttcataatac tgactagaaa tatctctctg tgctaattcc
    28981 attttatctt gtagtatatc tttcacttcg tcaaatacct tttcatttga ccataaacaa
    29041 attttatgta catcttgtga cttatactct tcaatattat tttcatatgt tattttttct
    29101 tggatatgtt gtttatttat acaagaatta gttcctttca actgattcat tgtttcaaat
    29161 atttctttgg ctttttgatt catgaaaact ttttcctgac tctcaatcga aaaagcaact
    29221 tctctctttt ttaacaaaca aacaacttcc tttatcaatc tttgattaaa agattgatta
    29281 taaagtaatt caccatgata ttgaatgtaa ttacctcctc ctgcaatata tccatcaacc
    29341 cctaatgata aaacatcatc ttgtattgta cctatacttc ttccagtaca aataacaaca
    29401 ctgcaatgat tcttttgaca taaacgtata gcgtgctttg cagattctgg gattccatat
    29461 acctcatccc taagcgtacc atctatatca agtattaata atttgtacaa tttcatcttc
    29521 ctttcttact atcaaaatca taatagcatt caaaaaaaaa gatatcaata ttttgatatc
    29581 ttttaaaaaa tatgttcatt tttagcacaa attttcactt tcggggaata tgttcacttt
    29641 ctaaatacat cttgtattta atataaagca gttcaattaa aataaaataa ggtgttgtta
    29701 catcgtagtc cagttgttta tctacagaaa tagatataga ttgaatatac aggttttcat
    29761 cacataatct tgctaaagga ttacttatca tttttgtaat agaaaccata taagttcctt
    29821 tcattctcat ttctttagca aaatcaataa ttgtagatgt atctccattc aatgagataa
    29881 agaaaatcat atcatctgtt tttataaaat gattaagcgc ctgaatcata tcataaccat
    29941 aaacatcata tataattttc cctgttggta agaaaatcct tttcatctct ttaacaactc
    30001 tttcctgaga ataaccttct ccaaagacga ttatacgatt tgcctttttc attctttcaa
    30061 acataatcga acaatccttc ttctctatat catctatcac cttattataa caatccatta
    30121 attggtgttt actttgattt ttatattcta cttctgtttc atttaagcgt ataataactc
    30181 ttaattcact atatccaggt aattgtagtt tttgagcaaa acgagaaagt gcacttgtag
    30241 atatatggta tttgtctgca aattcatcta tagaaagttt aatacaatct tctttatgat
    30301 taagaataca ctcacagata tacttatcat ttgctgtaaa tgaattataa tatttattga
    30361 aaagtccttc aattttcatt cttttctcct tctatactct atgtaattca tatgaagcaa
    30421 atctaataga gcataaaatg ctcctacgac ttcataagat atagcttgta atgtatcaag
    30481 tttttgagtt tcaacataca gattatactc acaaagtgtt gcaattgtat tattatcaag
    30541 cctagttaaa gaaatcgtat gtatatgatt gttgataaat tttgcaattt caattcctga
    30601 ttttgtttct ccagacaaag aaatgataat aaataaatca cttttttgaa atgattctct
    30661 cataaattca atttcaccta tatcaaataa gtcaataaca catttaccta ctgaaagaaa
    30721 tattctcttt atttcttgag ctaaactttt ctgttcatta cctgtcccat aaatataaat
    30781 tgtttctgca tcatatatta ttttacaaat atcttgaaat gatatttttt gtaattcatt
    30841 cattaatagt cgataattct cgcaaacatc atgaaaatta ttatcattat taacaaattc
    30901 tagttgatta tttttaagta aatactttaa atcagagaat gaatctaatc caatttttct
    30961 acaaagcctt aataaagtag ctctagaaac attacattca tttgcaattt tttcacacga
    31021 aaaattacgg tattctcttt gacaaatcaa tatttgttta ataataattt tatcattttc
    31081 atttaaacta ttataacttt ggttaactaa cgattctaat ctcatttttt ccactcccta
    31141 aatattattt taatatacat tagttgcttt ttctagtgaa gagtcttaaa taataaggat
    31201 aaaataatca tttgatttta tttttattaa aataaaaata acctgctaaa agcaggcacc
    31261 aaaaagtact tatcctttcg aacatgtact ttagatattg ctttcttcgg aagcttcatc
    31321 tcttcatact ctaaaataga gttttttcta actcaaaagt attttccatg gtagaaatta
    31381 ctttaattca tctgccattt attttttatc ttatttaata attactatta cttgaatatt
    31441 tacttcttta agtaaatttc atagttaaca aagctaaaac tattattata gatgttaaaa
    31501 tacaaaatta taagtattgt tataaatatg cttacagcta caaatataca tgatatatta
    31561 aaaagaagct gtaaattatt atcatacata caagcaacta tgcctctatt aatccatgtt
    31621 aatttttatt atttaataca aggagttcca tgagacatac atatccattc atattttaaa
    31681 ttatccaatt ctcttataga tttcatcatc tttttataat cccaattcca tggattagga
    31741 taaggaacta tatttccatt cttatttttg actaaatcac ctgcaaataa tacatcttca
    31801 aaaatcatgc agacatgccc tggagtatga ccaggagtat ggataatttt tatattccca
    31861 atcttcatat tctctccata aggttcaaca tccttaatga gccttctgga aattgccttt
    31921 ccaataaact tcttaaaccc aggtctatcc ttctctccta taataaaagg tatatcctct
    31981 acatgagccc atacctttgc tcctgttttc tctttcaata atttaatatt agatatatga
    32041 tcaacatcat gatgagttaa taaaatatgc ttaatatctg taagtttaat gccaagtgtt
    32101 tttaattctt taattatatt tttccccata aaaggaagac ctgtgtctat aagaataatt
    32161 tctttatcga acacaataaa acaatttgaa ccacgagtac tatctaacat taatacattt
    32221 tcttttatct tcataaataa attcccctta tttggtattt tacctatata attaattaat
    32281 aaagttataa atagttataa tacttgatat taattttatt ttttattaaa gtatactata
    32341 ggtatacaaa tattatattt acataagtat accatcagta tactttttta tcaatagaaa
    32401 ggaataattt atatgtcaga attatcaaaa agagataaag aaaaaataca aagagaaaat
    32461 gagattattg ataaggcaga aaaattattt tgtcttaatg gatttgatta aaaaatagct
    32521 acgcacactc gtgactttag tcatgagtta gtagctaaaa tagtcagcat atagggaaac
    32581 ctatatgtag agataggata a
//
LOCUS       DACTGW010000016        32521 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.016, whole genome shotgun sequence.
ACCESSION   DACTGW010000016 DACTGW010000000
VERSION     DACTGW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32521
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.016"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(115..924)
                     /locus_tag="I9573_000622"
     CDS             complement(115..924)
                     /locus_tag="I9573_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT4795256.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTFVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(1008..1541)
                     /locus_tag="I9573_000623"
     CDS             complement(1008..1541)
                     /locus_tag="I9573_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAT4795257.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGLNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(1668..3743)
                     /locus_tag="I9573_000624"
     CDS             complement(1668..3743)
                     /locus_tag="I9573_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4795258.1"
                     /translation="MLKGLSFRNARRQAGKYLIYWCSLIGSVALIYAFNALIFSDVMQ
                     ELSSILNNGGSDEFSYMIIIFSFIITFILGWFVSYMMNFILKKRSREISTYMILGVDK
                     KQIVKMLFIENLLLGASALIVGWILGIFVSKVLESIVINMFHGKYSLSPDFSLKAAGM
                     TFLYFCIIYLIAQLKSNKKLVKMKLINLLNYDRYNENVLIHQTKTKSIMFCVSVLCGG
                     MGIFFFSIPSGRLTDVFLGLLLIVFCLIVLFMGFGSIAQNMFEKGMNWKYKKSHLFLY
                     RLLASKINGISIIMGIIATLFTVSIACIGIANSFYSVMNKSVDLQQFDVSIFHPNESY
                     DFSSYEGYLSETKGVKKDYSYTLYTEEKTSFMKVRNNILSKYLKDIGRDLQYKDYTFA
                     ENQFDTFMKYSDYNILRNMLGLNPIKMDKNQFIIHCMPYLADAYKNYIGEKYKLTISD
                     KDLSIAGIYIENFSQYGGYGNGQEYIIVIPDYLIDNMKPLYSLYVAITSEPLDSAYFT
                     EFQKKFDTLENLNSGVVVAGNQGFVSKLRYDNKDYISGKYAMQSTGQAIILILPLFYL
                     ALIICIIGTVILSIQLLSENNIIIKHYGMLRTLGMIDSEVIKILKKHVCLYFSIPLIP
                     AVIFGGILVSVFSYNMFNISFDAPVFFNIQIPILHAVAITLINFVVIYCIYVFITYVA
                     LRKEILHIK"
     gene            complement(3746..4492)
                     /locus_tag="I9573_000625"
     CDS             complement(3746..4492)
                     /locus_tag="I9573_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4795259.1"
                     /translation="MLLRVENIEKYYGNQKILTKALNDVSFQINTGEFVSIMGTSGSG
                     KTTLLNCIATIDTVSAGKIYLENQDITELSEENLTVFRRDNLGFVFQDSNLLDTLTLE
                     ENIALPLTIKKVSPVEITNSVYNIATQLKIEEILSKFPYEVSGGQRQRCAVARAVVTE
                     PKLILADEPTGALDSNSSKTLMELLETINTILEATILLVTHDAISASYTERILFLRDG
                     KIFNELRKGEKSRLTFYHEILDALSLMGGD"
     gene            complement(4506..4901)
                     /locus_tag="I9573_000626"
     CDS             complement(4506..4901)
                     /locus_tag="I9573_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAT4795260.1"
                     /translation="MKKRILTILCVVLVLFISITGLDSMGFFSRSIIPLRSEYASFIR
                     KNGTITEVKFTGPFNHNNIDLNKNVEDTNKNGDDNELTIKYDIEVKKSLISSNEPTIQ
                     IQDSKESIYVYILNFSDKTIIIKNGKIVD"
     gene            complement(5014..6036)
                     /locus_tag="I9573_000627"
     CDS             complement(5014..6036)
                     /locus_tag="I9573_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4795261.1"
                     /translation="MRFCNFLQDKVLVILLNICCAISLVLYLHLIGIGQGELTLLIAA
                     WVAILFCWICFCYQKQKKEIIKLRRIVDELDKKYLFIEMIEKPTSNIEREYYNIIRLS
                     LKSMTDHVSQIHREMYEYRQFIEQWVHEIKHPVTTSELICENYKNEHTRKIRFQMEEI
                     ERHIERALYYARLGHVEKDYLISEITLKSIVEEVLAKNKLLLIQNNIHINIQDLEYSV
                     YTDKKWIVFILNQIIINSVQYKSDSAEISFFAIKEDKSVKFTIKDNGIGIKESEIYRI
                     FELGFTGSNGRTTNNSTGIGLYLCYELSKKMGIDIDVSSSLGEYTAITLNFPINTAIN
                     MQKQCN"
     gene            complement(6033..6704)
                     /locus_tag="I9573_000628"
     CDS             complement(6033..6704)
                     /locus_tag="I9573_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4795262.1"
                     /translation="MILTNILIIEDDVFICKELMQFFENNGYSTQTLDTNNCDVLQVI
                     QESTPDLILLDINLPNIDGFSLCRQIRNCSTIPIIFITARDSAIDELNGLTMGGDDYI
                     TKPYNLPLLLARVKNLLKRVDSSDKQKIKYREIILNLISATVEYQNAVIELSRTELKI
                     MYYLFQNVGSIVPRIDLVEYLWDNHIHIDDNALSVNITRLREKLRGIGITDLIITKRG
                     MGYRI"
     gene            complement(6790..8139)
                     /locus_tag="I9573_000629"
     CDS             complement(6790..8139)
                     /locus_tag="I9573_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HAT4795263.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNRANSIEEIKPCEISKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIDKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(8151..9533)
                     /locus_tag="I9573_000630"
     CDS             complement(8151..9533)
                     /locus_tag="I9573_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HAT4795264.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            9777..10991
                     /locus_tag="I9573_000631"
     CDS             9777..10991
                     /locus_tag="I9573_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT4795265.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIKLYGNKCDYAKRDTLLYTANKLEIKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            11189..13081
                     /locus_tag="I9573_000632"
     CDS             11189..13081
                     /locus_tag="I9573_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HAT4795266.1"
                     /translation="MKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLKEGIPLAE
                     KSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEPFGIIGFN
                     NVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAHIVKRLNC
                     KGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVDKNFKITL
                     FNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTKIITNRVP
                     IIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDNIIHASEKTKDCIEL
                     AKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSLIESEFFG
                     YEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQVMRIGGDK
                     VIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDIIKLSEFFI
                     HKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSILLEKVSNE
                     YTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRSTLWRKLK
                     EIEDDV"
     gene            13352..14326
                     /locus_tag="I9573_000633"
     CDS             13352..14326
                     /locus_tag="I9573_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HAT4795267.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            14347..14622
                     /locus_tag="I9573_000634"
     CDS             14347..14622
                     /locus_tag="I9573_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HAT4795268.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            14635..15798
                     /locus_tag="I9573_000635"
     CDS             14635..15798
                     /locus_tag="I9573_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HAT4795269.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGTEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            16135..18414
                     /locus_tag="I9573_000636"
     CDS             16135..18414
                     /locus_tag="I9573_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT4795270.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVNFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSVVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPMPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(18509..19849)
                     /locus_tag="I9573_000637"
     CDS             complement(18509..19849)
                     /locus_tag="I9573_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT4795271.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILIGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVTYYFIR
                     GKSTLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     IVQVFPEVFVGIFNKDSDLMDIAVRGISINLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(19861..20643)
                     /locus_tag="I9573_000638"
     CDS             complement(19861..20643)
                     /locus_tag="I9573_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017825045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4795272.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(20658..21680)
                     /locus_tag="I9573_000639"
     CDS             complement(20658..21680)
                     /locus_tag="I9573_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAT4795273.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(21686..22699)
                     /locus_tag="I9573_000640"
     CDS             complement(21686..22699)
                     /locus_tag="I9573_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003370238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAT4795274.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(22827..23765)
                     /locus_tag="I9573_000641"
     CDS             complement(22827..23765)
                     /locus_tag="I9573_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT4795275.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWSQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            24035..24499
                     /locus_tag="I9573_000642"
     CDS             24035..24499
                     /locus_tag="I9573_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795276.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTGKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(24611..24928)
                     /locus_tag="I9573_000643"
     CDS             complement(24611..24928)
                     /locus_tag="I9573_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAT4795277.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(24941..25672)
                     /gene="chbG"
                     /locus_tag="I9573_000644"
     CDS             complement(24941..25672)
                     /gene="chbG"
                     /locus_tag="I9573_000644"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAT4795278.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVVEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(25698..27008)
                     /locus_tag="I9573_000645"
     CDS             complement(25698..27008)
                     /locus_tag="I9573_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAT4795279.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNNGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLVAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(27085..28398)
                     /locus_tag="I9573_000646"
     CDS             complement(27085..28398)
                     /locus_tag="I9573_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT4795280.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(28420..28728)
                     /locus_tag="I9573_000647"
     CDS             complement(28420..28728)
                     /locus_tag="I9573_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017435008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4795281.1"
                     /translation="MKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKNE
                     IENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSSK
                     "
     gene            complement(28931..29635)
                     /locus_tag="I9573_000648"
     CDS             complement(28931..29635)
                     /locus_tag="I9573_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4795282.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(29730..31091)
                     /locus_tag="I9573_000649"
     CDS             complement(29730..31091)
                     /locus_tag="I9573_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT4795283.1"
                     /translation="MKDRINLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     MSE"
     gene            31590..>32521
                     /locus_tag="I9573_000650"
     CDS             31590..>32521
                     /locus_tag="I9573_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT4795284.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQDVINKKYIVYRIGGDEY"
BASE COUNT        11469 a         5367 c         4129 g        11556 t
ORIGIN      
        1 gaacttagtt acaagttcta aaaccctatt tgtttagtgt aaaaaacatc ataaaattta
       61 gcttaaatct tttcacataa tattactctt tttctaagtg ataataaaat atatctattc
      121 ctctaaaaac tgtaaaaatt cctgattaaa tagtttaagc tcatcataga atatagcatg
      181 accactatac tcaaaagtat ataaaattga atcttctatt ttttcattca taaaaactgc
      241 aaattcatac ggacatattt catctaattt cccatgaaat ataccagtag gcactcttac
      301 acattttaaa tcattaaata attcttcatc tcttaaagat acagcagttc caatagtgcc
      361 aatacctgat gcactaaaac caatatcatt aaaccatctt ctaaagcttt ctggtggatt
      421 tgatgcaaat actttttctc caaaatcggt taccatttct ggtctatcag tacatgcttg
      481 tgctatcaat ttatttacat cttcacgtgt cataccatat ggaaattctg gaggtctttg
      541 tacaaatgat ggtgcagcag ctgcagctaa taccaatttt gacactccat atccattaaa
      601 taaactcata tacctaagta cgattgctcc tcccattgaa aaaccaacaa aggtaaagtc
      661 ttttaatcca atagcatgta ccaccttata aatatcgtct gctaattggc tataagtata
      721 tcctcctgat gttgcatcag atttaccaaa acctcttaaa tcaattgaaa ctgttctata
      781 tccgagtttt ggcaatatat ttgtttgata ttcaaatatc ttatgtccta agggccaacc
      841 atgtataaaa agtacagttt ttttagcact cggattcaaa tcatatactg ctatgtttat
      901 atcattcaca tatacataat acatatcaac agcaactccc ttcaagaaaa tgtatgatgg
      961 ttattttata tatatgaata aaatgatgaa taactaaatt tttaaattta tttacaatac
     1021 ttctctattg ccttttcaaa taaacttaca tgaagttttt catctaggat tattctattt
     1081 aatatagctt ttatataagg gtcttcaata agacttatat ctttataata attattaata
     1141 gccacatgct ccaaatgcaa atctagtttt aatctgttac aaatagaatt tccataacat
     1201 acaaaaccac cattccaata tgcaccacaa gaacttgtag aacctctata tacaggattt
     1261 ccacccaata atcttatagt ctttgctaat atctccatat gtaacatttc tgttatagat
     1321 atatttatcc acatttcact taactctcta tgcgtttcat ctaaatcaaa attatggtat
     1381 agatattgat ttacactcgt aaattcactt gatactccgg catagtcatc catcaataat
     1441 tctgcataat acttatttag acctaaaact tttatctctg gatatggctc atcacttgaa
     1501 tatccatttc gcttgtgcat gtcgtaatgt gcatcactca taatatctcc tcctttaaat
     1561 ttaatacttg atattaattg tatgcatcac gactttttca tataacttag ataataaaat
     1621 ctattatatt attttctatt ataatttatg tgtcaattca cccaaattta tttgatatgc
     1681 aatatttctt tccttagtgc tacatatgta ataaaaacat atatacaata tattactaca
     1741 aaattaataa gcgtaatggc tacagcatgt aagataggaa tctgaatatt aaaaaatact
     1801 ggagcatcaa aagaaatatt aaacatgttg tatgaaaaaa ctgataccaa tattccacca
     1861 aatattactg caggaattaa tggaatcgaa aaatataaac aaacatgttt ctttaagatt
     1921 tttattactt cactatcaat catccccaaa gttcgaagca ttccatagtg ttttatgata
     1981 atgttatttt cgctcaataa ttgaatagag agaatgactg taccaataat gcatataatg
     2041 agtgccaaat aaaaaagtgg taatatcaag ataatggctt gaccagtaga ttgcatagca
     2101 tactttccac ttatataatc cttattatca tatcgtaatt tagaaacaaa gccttgatta
     2161 cctgctacta ctactccaga attaagattt tctaaagtat caaacttttt ctgaaactca
     2221 gtaaaataag cactatccaa aggctcactt gtaatagcaa cataaagact atacagaggt
     2281 ttcatattat caattaaata atctggaatg acaataatat attcttgtcc atttccatat
     2341 cctccatact gtgaaaagtt ctctatataa atacctgcaa tacttaagtc cttatctgat
     2401 attgtcagtt tatatttttc tccaatataa ttcttatatg catcagcaag ataaggcata
     2461 caatggatga taaattgatt tttgtccatc tttattgggt ttaaacccaa catatttctt
     2521 agtatattgt aatcactata tttcataaat gtgtcaaact ggttttctgc aaaagtataa
     2581 tccttatatt gtaaatctcg ccctatatct ttcaagtatt tagaaaggat attattacga
     2641 accttcataa aggatgtttt ttcttctgtg taaagagtat aagaataatc ctttttcaca
     2701 cctttcgttt ctgataaata tccttcatat gaagaaaaat catatgattc attcgggtga
     2761 aaaatactca catcaaattg ttgtaaatcc actgatttat tcatcacaga ataaaatgag
     2821 ttagctattc caatacaagc aattgatact gtaaaaagag ttgcgataat acccattata
     2881 atgcttattc catttatctt tgaagccagt agacgataca aaaataagtg agatttctta
     2941 tatttccaat tcatcccttt ttcaaacata ttctgtgcaa tagatccaaa tcccataaat
     3001 aatacgatta gacaaaatac aatcaacaat aaaccaagaa atacatctgt taatctacca
     3061 ctaggtattg aaaagaaaaa aatacccata cctccacaca aaacagacac acaaaacata
     3121 atagacttcg tttttgtttg gtgaattaat acattttcat tatatcgatc ataattaagc
     3181 aaattaatta atttcatttt aacaagtttt ttattacttt ttaattgagc aattaaatat
     3241 attatgcaga agtataaaaa agtcatccca gcagctttta atgaaaaatc cggagataaa
     3301 gaatactttc catgaaacat attaataaca atagattcca aaaccttaga aacaaatatc
     3361 cctagaatcc atccaacaat aagagcagaa gcacctaata aaagattctc tataaaaagc
     3421 attttaacaa tttgtttttt atctactcct aatatcatat aagtacttat ttctctgctt
     3481 ctttttttca atataaaatt catcatataa ctaacaaacc agcctaaaat aaaagtaata
     3541 atgaacgaaa atatgataat catataagaa aactcatcgc ttcctccatt attaagtata
     3601 ctagaaagtt cttgcattac gtcggaaaat atcaaagcgt taaaagcata aatcaaagct
     3661 acagagccta ttaaggaaca ccaataaata aggtactttc ctgcttgtcg tcttgcatta
     3721 cgaaaagata atcctttcaa cataatcaat ctcctcccat caaagaaagt gcatccaaaa
     3781 tttcgtgata gaaagtaagt ctggattttt ctccttttct aagttcgtta aaaattttcc
     3841 catcccttag aaataaaatt ctctcagtat aactagctga aatagcgtcg tgtgtgacaa
     3901 gaagaatcgt agcctctaat atggtgttta tagtttccaa aagttccatt aaagttttag
     3961 atgaattaga atccaatgca ccagttggtt catctgccaa aataagtttg ggctctgtta
     4021 caactgcacg agctactgca caacgttgcc tctgaccacc agatacttca taaggaaact
     4081 tcgaaagtat ctcttcaatt ttcaattgtg tggcaatatt atacacacta ttagtaattt
     4141 caacaggaga aacttttttt attgtaagtg gaagtgctat attttcttct aatgtcagag
     4201 tatctaataa attagaatcc tgaaaaacaa aacctaaatt atcacgacga aaaacagtta
     4261 agttttcttc ggatagttca gtaatatctt gattttcaag ataaattttt ccagcactta
     4321 ctgtatcaat tgtagcaata caatttaaca aagtagtttt accagagcca gaagttccca
     4381 taatactgac aaactctcct gtattaattt gaaatgacac atcattcaag gcctttgtaa
     4441 gtattttctg attgccataa tatttctcaa tgttctctac tcgtaatagc atgttaaagt
     4501 cctccttagt caacaatttt tccattttta attattattg tcttatctga aaaattcaag
     4561 atatatacat atatgctttc tttagagtct tgaatttgaa tagtaggttc attagatgaa
     4621 attagagatt tttttacttc aatatcgtat tttattgtta attcattatc atctccattt
     4681 ttattagtat cttctacatt tttatttaaa tcaatgttat tatgattaaa gggaccagta
     4741 aactttactt ctgtaatagt tccatttttt ctaataaaag aagcatattc agaccttagt
     4801 ggtatgatac ttcgtgagaa aaaacccatc gaatccaatc ctgtaattga aataaacaaa
     4861 acgagtacaa cacataatat agttaatatc ctttttttca tatgtttctc cctcctttct
     4921 attgatattg tagcgtaaat agtaatctga atatatagaa gttacttaac tttatcttac
     4981 agttttgaaa gataaagaaa tagcagcgca tttttagttg cactgcttct gcatattgat
     5041 agctgtattt attgggaaat tcaatgttat tgctgtgtat tcaccaaggc tggaggacac
     5101 atctatatca attcccattt tcttagacag ttcataacaa aggtaaagac caatccctgt
     5161 agaattgttt gtagtccttc cattactgcc agtaaaacct aattcaaaaa ttcgataaat
     5221 ttcactttct ttgatgccaa taccattatc ttttatagta aacttaacac tcttgtcttc
     5281 ttttattgca aaaaatgaaa tttcagctga atcacttttg tactgaacac tattgataat
     5341 aatttgattt aaaataaaaa ctatccactt tttatcagta tatacactat actccaaatc
     5401 ctgtatatta atatgaatat tattttgtat aagaagcaat ttattctttg ctaatacttc
     5461 ttctacaatt gactttaatg ttatttcaga aattaaataa tctttttcaa catggccaag
     5521 gcgagcatag tacaatgcac gctcaatatg acgttctatt tcttccattt gaaaacgaat
     5581 ttttcgtgta tgttcatttt tataattttc acatataagt tctgatgttg taacaggatg
     5641 ttttatctca tgtacccatt gttcaatgaa ttgtctatat tcgtacattt cacgatgtat
     5701 ttgagaaaca tgatctgtca tagatttcaa tgataatcta ataatattgt agtattcacg
     5761 ttctatgtta gaagtaggtt tttcaatcat ttcaataaac agatattttt tatccaattc
     5821 gtctacaatg cgacgtaact taataatttc ttttttctgt ttttgataac aaaaacatat
     5881 ccaacaaaat aaaattgcga cccaagcggc tataagaaga gtcaactctc cttgaccaat
     5941 ccctataaga tgaagatata gcactaatga aattgcacaa caaatattta agagtataac
     6001 taatacttta tcttgaagaa aattacaaaa cctcatatcc tatatcccat cccccgtttt
     6061 gtaatgatta agtcagtaat acctataccc cttaactttt ctctaagcct tgtaatgttt
     6121 acacttaaag cattatcatc aatatgaata tgattgtccc ataaatattc taccaaatct
     6181 atacgaggca caatactccc tacattctga aacaaataat acatgatttt tagttcagtt
     6241 cttgaaagtt ctataacagc attttggtat tctactgtag ctgaaataag gttgagtata
     6301 atctctctat atttaatttt ctgtttatct gatgaatcta ctcttttaag taggttcttt
     6361 acccttgcta aaagcaaagg taagttatat ggttttgtaa tatagtcatc tccccccata
     6421 gtcaaaccat ttagttcatc aattgcacta tctcttgctg taataaaaat aataggtata
     6481 gtagaacaat tcctaatctg tctacataaa gaaaaaccat ctatattagg caaattgata
     6541 tccaagagaa ttaagtcagg tgttgattct tggattactt gaagtacatc acaattattt
     6601 gtatccaatg tttgtgtact atagccatta ttttcaaaga attgcattaa ttccttacag
     6661 atgaacacat catcttcaat tatcaaaata ttggttaaaa taataaatac ctcatttctt
     6721 tagtttatat tgctttaatt ttgtaagtaa tatatttttc catatattac ttacaaaatt
     6781 actttttaat tattcatctt ttccagctac tgatagctct ttaataacaa ctgaaggtga
     6841 acctatacta ctcattggaa attctaaatc ttcccctata acttcaatat ctttcaacag
     6901 gtcaaaatag tttcctgcta ctgtaatttg ctccactgga aatacttttt ttccttcttt
     6961 gatataaaag cctttggcag ctaaagaaaa gtctcctgtt actgaatttg cacctgaatg
     7021 aagccctgca aagctagtaa ccatcaatcc ttcatctata tcctttataa tttcatctaa
     7081 agatttatct cctttatcta tataaaaatt agttggatat atgcttatag gtgatgaata
     7141 tgaagatttt attccatttc ctgtagtctt tacacctgcc ttattagccg tttttaagtt
     7201 atgaagtaaa gttataagct ttccatttaa aacaacttct tttttaaatg tagctactcc
     7261 ttcatcatca aaaggagtag atgccatacc attttctaaa agtgggtcat caactattgt
     7321 aactatagga gatgctatca tgtctccttc tctatttttc aataaagata atcctttttg
     7381 agctgaatct gcattaaata tcccagaaaa tgtgctaagc aaagatacca tagcttcatt
     7441 aagcaatata gttttatatt tacctgatgg aatactctta ccacctactt tagataaagc
     7501 attatcaaca ccaagttttg atatctcaca aggttttatt tcttcaatag aatttgctct
     7561 attatatcct attccatcat atttttgtcc attgtcttct atgataggaa ctacaaaccc
     7621 tataagcata ttagttttat tgcttaaatt aagaccttta gtattagata taccattact
     7681 tgatacagaa taagatatag tacatttaga taaatttacc accttatctg aataagcttt
     7741 agtctctctt tccatttcta aagctaaatc aatcaactta tctgcctcta aattttctag
     7801 ctctttagaa taagtcttta catcattata gtgcttatct ccttcatata taaattgtac
     7861 atcttcattt tcaatagtgt ttactccatc ttttacattt tttatcaaca tatcaacagc
     7921 tttatcatct aaaatttctg tataagaata acctatcttt ccatttacct tacctctaaa
     7981 agataaacca aaagatttat caagattgta tttctcaact tctccttcat atatatttat
     8041 acttaaattt tctcctgtat aataatatat ttcacactct tcaaaacctt cacttagagc
     8101 ctttttaaat aatatatctt taaaattttt aagttccaca attatccctc ctatcttcca
     8161 cctacagtaa tttcttttac ccttatcata ggttggccta cattagttgg tatacttcct
     8221 gatgatgagc cacacatccc ctgagcttgt tttacgttat tcccaaccat atctatattc
     8281 ataagtatat cgctaccttt tccaattaaa ctagcacctc tcactggctc ttgtatttct
     8341 ccatttttta ttaagtaacc ttctgaaact gcaaaattaa actctcctgt tacaggattt
     8401 acagaaccac cacccatttt tttagcataa agtccatctg gtattgactt tataatgtct
     8461 tctggtctat cttctccagc agcaatataa gtgttagtca tccttgaagt tggtgcaaat
     8521 ttgtagttct gtctacgaga acttcctgta ggattcattc ccattcgtct accattaagt
     8581 ttatctatca tataagactt caaaattcca ttttcaatca gtatattctt ttgagttgga
     8641 tttccttcat catctatatt catagaaccc cagtagtttg gtattgtacc atcatctata
     8701 gctgtaatct ttgtggaagc aatctgttga cctaatttat ctgcaaatac agagttcccc
     8761 tttgcaactg atgtagcttc caaagaatgt ccacaagctt catgaaatat tactccacca
     8821 aatccattat ctattgctac catcatatta cctgcaggac aatttttagc atgcaacata
     8881 gtatgcgcta ctctagctga ttcttttgca taatattctg ggtctatttc ttcaaatatt
     8941 tcaaaccctt tacaacctcc aggaccttca aatccagttt gattttcatt acccttagat
     9001 gcaattgaac ttatccctag tctggttctt attcttctat cctcaacata tataccttca
     9061 gtgtttgcaa ttaatatttt ttggtcttta tccaaatagc taacacttac ttgagatata
     9121 tcattactaa attcttttgc actcttatat gcaaccttca taacacttac tttatctcta
     9181 tttcctattg aatttggata aatttttata ttatgtatgt tatttatttt aatactgtca
     9241 tttaatatta cactttttcc atcttccaat ttacctaaag caacagcagc tttgtatgct
     9301 acatccaaca gactatttaa tgaattgtca cttgtataag catatatact cttcaatcct
     9361 ttaaatattc ttattcctat accataattt cttccaccta ttgcattttc tactttatta
     9421 tcaattaaac taatagaatt atttatagtg tcttcttcaa aaatctcagc aaaatctcct
     9481 ccagtaatta gacacttttc taatacctta gaagctatat tttttgaaag cataaattta
     9541 cctcctactt taaattttta attaactttt attcaatctt tttacttgtt aacaaacatt
     9601 atatactata ttacttaaaa taaggtaatt atgaatatat tatacatatg ttttatacta
     9661 aaatgttatc acattttatc tatacatacc catataacta gataattaaa atgtataata
     9721 ttatttgcta tagaaaataa tacactcata cataataaat tttaggagtg aagtatatga
     9781 atcaagaata tgtaaaagga aatcctattt ttactaatat taataaagtt ccaagacaat
     9841 ttccatacct tactgatgat attgatactg atgttataat tgttggtggt ggcgtaactg
     9901 gatgtatttg tgcttactat ctagctaaaa acaatataaa gtctgtcatt cttgaaaaag
     9961 gaaggattgc acatggaagc actagcgtga caacttctct attacagtat gaattagatg
    10021 acaatttgat agatttaaca gaggttatga ctctaaaaga tgctctaaaa gcatataacc
    10081 tttgtacttc agcacttgaa gaattagata catttataaa gctatatgga aataagtgtg
    10141 attatgccaa aagagatact ctactttata ctgccaacaa acttgaaatt aaggctatca
    10201 aagaagaata taacttaaga aaagaaaatg gatttgatgt tgaatatatt gatgaatcca
    10261 caaacccttt tagctttgat ttaaaatcag gattgatagc aaagaatgga ggacgagaat
    10321 tagacccata taaatatagt caccatttga ttgatgtaag tttaaaaaat ggtcttcaag
    10381 tatatgaaaa tacagaagta aaaaaagtag atttatcaaa tgataaggtt actgctgaag
    10441 tatcgtatgg atacaaagtt catggtaaaa aattaattgt agctacaggg tacaatacaa
    10501 gtttatttac aaaaagaaat tttgcaacta agtcaaatac ttttaatata gcaactaaac
    10561 cacttaaaaa catagcttct tggaaaaaca acattttaat tagagataac tgtgacccat
    10621 ataattatct aagaactact aaagacaatc gattaattat aggtggtgaa gatgtaagtt
    10681 ttgatgatat agaaaatgaa actttagcca atgaaaagta tgatatttta gaacaaagat
    10741 tgaaaagtat gtttaaggat ataaaagaca ttgaagttga atataaatat tgtggttgtt
    10801 ttgcttcaac tttagataat ttaggtttta ttggtccaga taataaaaat aacaatctat
    10861 ggtattgctt aggttatgga gcaaatggta tactctttgc aatattagga ggtataatgt
    10921 taagtgaatt atatcttggc aaacaaaata aaaatatgaa attatttaaa gtagatagat
    10981 ttgataaata gatattgtat attaaaagac tataaaaaga tgaaacttta taattctaaa
    11041 agttaacttt gatacagtct tttataatgt tccttttaaa aatactttat ttataattaa
    11101 atataaagta aaatgttaaa atataactga taaaatttag tcataagata atatttaagt
    11161 ttatttgatg aaatatggag gaattattat gaaagaaata ctcgttattg ctcccactaa
    11221 aggaacctat gaaaaatcaa ttcacatagt taaaaaaaac aaatatacaa atattgatgt
    11281 tgtatttggc aatctaaaag aaggtatacc tcttgctgaa aaaagtatta atcatggtac
    11341 tagaattatc ataagcagag gtggaactta caatatgtta aaagctactt acaatatacc
    11401 tatagttgaa attaaagtag atgcttatga tattataaaa agttacaaag aggtaaaaaa
    11461 ttccaacgaa ccatttggaa taattggatt taataatgtc atctacggtt tcgatattat
    11521 agaagaaata ttgaataaga aaataactat gattgaaatt gaaaaagaag aagaaattta
    11581 tgatgctata gaaaagtaca gaaagaaagg tataaacaca tatataggag ataccactgt
    11641 tgcacatatt gttaagagac ttaattgtaa gggaatatta atcgaatcta gagaagaaaa
    11701 catacttaga gccattcaac aagcagaaca aatactcgaa gcaaccaagg atgaacaaaa
    11761 aagaagatta cagatagaag ctatgacaga ttttgtacat gatggaataa taacagttga
    11821 taaaaatttt aaaataacat tatttaataa gagtgctgaa aaaatatttg gtataaaaaa
    11881 agaaaatgca ctttatcata atgttgtaga tgttatccca aacacagttc ttccacaagt
    11941 agttgagact atggaacctc aaattggtga aatacaaaat atagggagta caaaaattat
    12001 tacaaataga gttccaataa ttgttgatgg ccagattcaa ggggctgttt caacatttca
    12061 agatagcaag gagataagtt cttttgaaca tacaataaga aggagcttat taagcaaagg
    12121 tctatctgct aaatacactt ttgataatat aattcatgct agtgaaaaga caaaagattg
    12181 tatagaactt gcaaaaaaat atgcttgtta tgatactcct atgattatta ctggagaatc
    12241 tggagttggt aaagaattat tttgtcaaag tatacataac tatagtaagc gtaaaaatgc
    12301 accttttgtt gctgtaaatt gtgctgccat acctccatct ctaatagaga gtgagttttt
    12361 tggttatgag gaaggttcat ttacaggtgc tagaaaaaaa ggtaaagcag gtgtattcga
    12421 actagcacat gaaggtacaa tcttcctaga cgaaataagt gaaatccctc ttgaacttca
    12481 aggtcgatta ttgagggtat tgcaggaaaa acaagttatg agaattggtg gtgataaagt
    12541 tattcctata gatgtaaaaa ttatctgtgc ttcaaataaa gacttaaaaa atatgatgcg
    12601 caatggttta tttagacaag acctttattt tagaataaat atactaacat tatatcttcc
    12661 ttcattaaaa gaaagaaaag aagatattat aaaattatca gaatttttta tacataaata
    12721 ttcaagcaaa tacaataaaa cgcctttaat tatcactcca aatattaaaa aggcacttct
    12781 tgaacgagaa tatgaaggta atataaggga attggaaggt atgatggaaa gggctgttat
    12841 tgtggaatca tttgactcga ttttattaga aaaggttagc aatgaatata caaataatat
    12901 agttgataaa gataatagtg attttgaatg cagtaattta gatttaaaaa ctttagaaaa
    12961 aaaatatatc tataaaattt ataacgaaaa taatagaaat actacaaaaa cctgtgaaat
    13021 tctaaagata actcgctcca ctctatggcg taagttaaaa gaaatagaag atgatgttta
    13081 aaaataatac atacctatat atatcaaaac gttttatgtt taattttaat acataaggcg
    13141 tttatttgtt ttcaataaaa aatacaatta tatgataaaa agaatctttg taagtgaaat
    13201 tctcatcaat cataccttta attataagta atctacagca tttcaatcga ttattaaaaa
    13261 taatcatgat aactaaaaat acaaactcta tatattggca tagtaattgc ttttataata
    13321 cctatataaa aatgaaaggg agtgatacta gatgcacatc ttaaaatcag tgaaaaagat
    13381 accaggagga cttatggtct gtcccttgtt attgggagca ctatttaata cagtatgtcc
    13441 acaagctctt gaaattggag gattcacaac atccttattt aaaactggtg caatgagtat
    13501 acttgctcta ttttgtctat gcaatggagc tcaaatcaat atcaggcaag ctggtaagcc
    13561 attgactaaa ggtatagtac ttacagcaac aaaatttata attggtgcag tattaggtat
    13621 tattgttagt aaatttattg gacctaaagg tataattggt attacacctc ttgccatagt
    13681 tgctaccatg actaattcaa atggaggtct atttgctgct cttgcaggtg aatatggtga
    13741 ctccacagat gttggagcaa tatctatctt atctctaaat gatggtcctt tttttacaat
    13801 gcttgctttt ggagtaactg gacttgcaaa tgtacctata gtagcactat ttgcagcact
    13861 tataccaata ctacttggat ttatacttgg taaccttgat gaagatatac gagaatttct
    13921 tgcacctggt acaacattat taattccttt cttcgctttt cctttaggag cagcattaaa
    13981 ctttaatcaa attataactg ctggactacc tggagttgta cttggtttag gagttactat
    14041 aataacaggt atgggtggct actttgtaat gaaacttata cgtgcagaac atccagcagt
    14101 aggtgcagct acaggtacaa cagccggaaa tgcagctggt actcctgaag ctttagcagc
    14161 tatagaccct actctagcta caatagcagc tgtttcaaca gtacaggttg ctgctgctat
    14221 tattgttaca gcaatatgtt gtccaatgtt agtttcctat ctagataaaa gagaaaaaaa
    14281 gaaaaaatct atttttaaaa caaataaaaa tattgaagta aattaatact taaggaggta
    14341 aaattaatga atgcaattat tcatgcaaat gaaaaagata atctagttac atgtgtcaga
    14401 cctttagtta aaggtgaaaa agtttcaatt gatggaaagt cttacatagt aaatgataat
    14461 atacctgtct ttcataagat ggctacagaa gatgtaaaaa aaggtgatgt tgtatacaaa
    14521 tacggtgaaa ttataggaat tgcaacagtt gacataaaaa ctggtgacta tgttcatgta
    14581 cataacattg aaagtacacg tggtcgtgga gataaaaaat aggaggaagt taaaatgaaa
    14641 ttaatgggtt atttaagaga aaatggtcag tttggtattc gtaatcatgt tttaattatt
    14701 ccaacatctg tatgttcaag tgagacagct acaagaatag catcacttgt gcctggagca
    14761 atagcaatcc ctcatcaaca tggatgttgt caaattggtt ctgatataga gcttacagca
    14821 aagacattaa taggatttgg aaaaaatcct aatgttgccg cagttttagt agttggtctt
    14881 ggatgtgatg gtattcaagc aaaagaatta gcatctgaaa tagctacaac aggaaaaaag
    14941 gtagattacg ttgttataca agaatgtggt ggaacattaa aaacagtttc taagggaact
    15001 gaaatagtaa gtaaaatggc tcgtgaagta tcaaaagaag taagagttga atttggtatg
    15061 agtgaaatta cattagcact tgaatgtggt ggttctgacc caacaagtgg aatagcttca
    15121 aacccttcaa taggcgttgc atcaaacctt cttgtagatg aaggtggaag tagcatacta
    15181 agtgagacta cagaagtaat tggtgcagaa catttgttgg ctacccgttt tgaagatgaa
    15241 gaaatgaaag ataagtttct taaatttgta agtgatgtag aaaaaagagc aattgctatg
    15301 ggagaagatt tacgtagtgg tcaacctact ccaggtaata aagctggtgg actttcaaca
    15361 attgaagaaa aatcacttgg gtgtatgtat aaggcaggca ataagccatt caaaggtgct
    15421 cttgaatatg ctgatattgt acctcctgat aaaaaaggat tatattttat ggatactcca
    15481 ggtcaagata tagattctat cactggtatg gtagcaggtg gtgcacaaat tgtaatcttt
    15541 tcaactggta gaggtactcc aacaggaagc cctatttcac cagttattaa aatcactggg
    15601 aatagtgata catacaacaa aatgccagac aatatcgata taaatgcagg tagaattata
    15661 acagatggtg ctaaaatagc agatataggt caagaaatat ttaatgaaat aatagaagtc
    15721 tgtaatggta aacataccaa agctgaaagt cttggtcata gagaatttgg catttacaga
    15781 atttcatcta cattttagat tgtaacaaaa tatcttttat aaatataata cttatgtaga
    15841 tacataagct aaaagttctt tctaatccta taaaaatttt ctatactgaa aaggaactgt
    15901 atcaaaatta ttttgaaaac agttcctttt ttaacctctt ttactcaaaa tattataaaa
    15961 taagatttaa acaataaacg tgtcttgatt aatctctaag aagacataaa taaagctaaa
    16021 tgtaaagtga gataaataaa atttaatatc tttgtatgaa aagatatttg gtataatatg
    16081 tctgagagaa ttcttatttg taaacaaata aaggatggag gatgaaacaa aagaatgaac
    16141 tttgaaaaca caaaaaataa aaaaataata gctactttac tattgacact ccttttaatc
    16201 gtgtcattaa catgtgtcaa ttttattatg aatctaaata aagtaacaca taatgaaaca
    16261 gaacgatatc ttgtagaagt ttcacaaagt atttccacta caattaatgt gaaatgtaaa
    16321 agtatattta ggacactata ttctatggga gatacttatt ttgagattaa tgatagtgaa
    16381 aaagaagtca atagatactt atcaaaaaaa ttaaaattat ttgaatttga ttggttaggt
    16441 attatagatt ctaatggaaa ttttgtaagt gcccttaaag atacaggtaa tgttaaagga
    16501 caagactttt atagaaaagc ccttcaaggt aaagaaacaa ttactcctgt taaatcacaa
    16561 tctggtacat atggaatttt atattctgta ccactataca atggcgataa aattgttgga
    16621 gctgttgctg cttggaatac tattgatact atgagaaatt tattaagtgt agaaagcttt
    16681 catggtgaag gattttccca aattatcgac caagatggaa attttattgt aagttcagca
    16741 aacaaaaatg ctccaaaaaa tgtggtaaat ttttttgaca tgattaaaca ccaaggaaaa
    16801 caggttgata aaaataaact tgaagaaatg aaaataaact taaaaaagaa caaaactggt
    16861 tctatgtatt atacattaaa tgatggtatt tctaaggcaa tgcattatct tccattacaa
    16921 gaagaaaact tatacctcct ttcagttgtt ccaacaaaaa cagcaagtgc acagtttgat
    16981 ttacttctaa aaaaagcaat cttcatcaat attttaattg ttatactatt tttattgctt
    17041 attatattga ttatgtttat aaatcaaaag aacagggcac aacttataaa aatcgcctat
    17101 gtagactctg taactaaagg ttacagtaaa gcacgttttg atattgaagc taaaaaaatt
    17161 atacagtcat cttcatcaga aagctattca ttggtatcta taaatattca gaaattcaaa
    17221 cttattaatg attcttttgg tagtgaagca ggagataaaa cattaaagta catacatgat
    17281 gtcattagta attatctaga accaggagaa ttgctttgtc gagtttctaa tgaccagttt
    17341 aatgtactta ttaaaactat tcctcaaaaa gaaattctta agtacataga aaatatcacc
    17401 aatgatatta atagtttcaa taaaaatgaa gaatgtaaat attttatagc tctatctata
    17461 ggtgtatata aaattgatga cccaaatcta cctttagttt caattcaaga ccgtgcaaat
    17521 gttgcacgaa aaaataataa aggaaacatt ggaaatcatc cactttatac atgtgttttt
    17581 tatagtgatt tagagcgttt aaaaatgatt aaagaaaaag aaatggaaaa tagaatggag
    17641 gaagcattaa ataataacga atttatagta tatcttcagc ctaaaataag cctcaaagat
    17701 aatactattg taggtgcaga agcattagta agatggcaag acccaaataa aggccttata
    17761 ccaccagacg agtttattcc tttctttgaa aaaaatggtt ttattacaaa actcgaccac
    17821 tatgtttttg aaaaagtatg tagtatgata agaaaatgga tagataatgg agaaactcca
    17881 atgcctgttt cagttaattt ttcacgtgct aatttaaata atatagattt tttagaaaaa
    17941 tacagaatgc ttcgtaataa atacaacatt cctgctaagc tcttagaaat tgaattaact
    18001 gaaacacttg tatttgaaaa tctccaaaaa ttaattaata taatagacca aatccatgaa
    18061 gaaggttttc aatgttcact agatgacttt ggaagtggat actcttcact caatatgtta
    18121 aaagaaataa aagttgacac attaaaactt gaccgtgctt tttttagctc acctaatgca
    18181 gataatattg ctgaaaatca tgtaatagaa tctatagtta atttggcaaa aaaattaaat
    18241 atgaatagta tatcagaagg tgttgaaaca atcttgcaaa tggattatct taaaaaaatt
    18301 aattgtgata tgatacaagg atatgttttt tcaaaacctc tgcctcaaga aatatttgag
    18361 aaaatgactt ttggaaaaaa actagaagaa accagtatgg atagttccgt ttaaataaaa
    18421 taaaaaactt gtctctataa aataatttta atcattttat aaagacaagt ctttttattt
    18481 tccaatttaa ttttattttc taaatttctt atactcggct tgttttaaat tcccttttta
    18541 agaataataa tacgattatc atagcaataa aatctgcaac tggctgtgcc aaccaaacac
    18601 catctaaatt aaattgaatt ggaagtacaa tgattaatgg tataaataaa ataaattgtc
    18661 ttaaaagagt taaaaacatt gaatgtttaa ctttacttat acactgaaaa tacacaggtc
    18721 ctacaataga aatccccatt ataggcaatg taattagatt tatgcttata cctcttactg
    18781 ctatatccat caaatctgaa tctttattaa atatacctac aaatacttct gggaaaactt
    18841 gtactattaa aaatccaaat gacaatatga caactgacac tatgatagat aatattaaag
    18901 ttttctttgc acgttcatat tgttttgcac cataattgta tgatattata gtttgcatac
    18961 cttgattcaa tccaaacaca ggcatcataa acatcaataa aatagatgta agtgctgtca
    19021 tagccccaat agctaaatct ccaccataaa cctttagaga attgtttgta aacatgtgag
    19081 ttatacttat agctacttcc atagcaaaag gtgtcattcc tattgcaaat accttcttta
    19141 ctatatcttt attcaatttt aagttacatt tttttagttt taaagtggac ttccctctta
    19201 taaaataata tgttacccaa aagaatacta ctatttgaca tagtactgtt gctatagctg
    19261 ctccttttat acctaaatta aacacaaata taaatatagg gtccaatatc aggtttaata
    19321 cacatcctac aatcattgtc cttgctgcta attttggatt accttctgct ctaattgcat
    19381 tattaagaga aaatgcaact aaattaaata tacttccaat caatatgaca ctcatatagt
    19441 cttttgcata aaatattgaa tctttacttg cacccaaaat aaaaagtata tcttctaaga
    19501 aaactaatcc taatatagtt ataattaaag agataattat agataatgtc aaagcattac
    19561 ctaatatctt ctctgcttct tctctatttc tttcccctaa ttttatagac aatcttgtgc
    19621 tagcacctac tgataccaac attccaaaag ctattattag agtaaatact ggcatagtta
    19681 caccaagacc tgcaatagct atagagccaa taccttccat agaaccaata aaagctctat
    19741 ctactacatt ataaagtgaa gttaccatca ttgctattat tgccggtatc gaatacctaa
    19801 ataataaaat ccatattttt tcatgtctta atgcttcttg attctccatg ttgtcaccat
    19861 ttacaccatg tcataagtta tacaaattgg tttatttgtt cttgggtctg ttgctatttc
    19921 tgcatcaata ttaaataatt cttttagatt atccttcgtc ataacatcat atgcatcacc
    19981 ttcacaaact attttacctt tcttaatacc tatcatgtgg tcagcaaatc ttgctgcatt
    20041 attaagttca tgtattacca tgacaatagt tcttccttgt tttttattta gttcatctaa
    20101 taattttagt atctctaatt gatgtgccaa atctaaatat gttgtaggct catccagtaa
    20161 taatatatca gtttcttgtg ccaaagccat agctatccat gccctttgtc tttgtcctcc
    20221 tgataaatct tccatatttc tatccttaaa ttctgcaatt ccagtaactt ctaaactcca
    20281 atttacaatc tctctgtctt ctttactaga attaccaaat ccacttttat gaggaaatct
    20341 tccataagaa attaattctg aaacggtaag accactagga gcttgagggc tttgtggaag
    20401 tacagccata tcctttgcta tttctttaga cttctttttg tagatgtcct ctccatttat
    20461 atatattttc ccactattag ggctaataat tctacctata gttttaagta ttgtcgattt
    20521 tccacaacca ttagccccta tgatggtagt tatttttcct tttggtatat ttaaatttaa
    20581 attttttata atatctgtgt ttccatatga tacatttaga ttcttagtat ttatacagtt
    20641 cataacaaca ccctttccta atcatttacc aacattaaat aaataaagta cggcactcct
    20701 attatagaaa ctactatacc tactggtatt tctataggtg ctagaagatt tcttgatata
    20761 gtatctgcta ttaaaagtaa taatgttcct atcaatgctg atactggtat taatctttta
    20821 tgctttggtc ctattatttt tctacctata tgtggagcta ttaaacctaa aaatgaaata
    20881 ctacctgcta ctgctgtcga tacaccagat aaaattactg catatattat aagtcttctt
    20941 ctttgttttt ctacatctac accaagactt atagatactt catcacccaa atttaaaaca
    21001 tctatatatt tagatttata aatagttata cccataaaaa tcactataaa tggaagcaca
    21061 gctattacat atttccaact ggttccccaa atgcttcctg atgtccatgc cataacccta
    21121 ttaaattctt gtgttgtaaa tttcaattgg aatatcgtca aaattgaagt aaaagctatg
    21181 tttacaccta taccaataag tagtaatctt gacgaattta taccaccttt ccatgcaaga
    21241 gaatatataa gaaatgctcc aaataaagct cctataagtg caacaatagg cattgtaaat
    21301 acagtcaagt tactcatacc ttcatataca ttaccattca taaggtatat ataaactact
    21361 acaaacaatg cagcacctga atttattccc aaaattccag aatcagctaa atcattcttt
    21421 gtaactcctt gaagtattac acctgctgtt gataatgcag aaccaaccaa aagtgctatt
    21481 actattcttg gtaatcttaa tttaaatatt gcaatttcat gattacgctg accctgccct
    21541 aaaaaagtct ttataacatc cattggttct atagaaaatg agcccatatt taaactcaat
    21601 aaaaaagtga taaaaataag caccattaaa attgatataa caagtaatgt tttagaattt
    21661 atttttataa tatttttcat agtacctatt tctccccctt tctaacaagg tatataaata
    21721 ttggcactcc tataagtgct gttatagaac ctactggtgt ctcatatgga ggatttatca
    21781 tcctacttaa tatatctgta tatacaagca atacagctcc taatactaaa gagctcggta
    21841 ttatatactt ataatctgaa cctactattc ctttagctat ttgaggaact ataagaccta
    21901 taaaaataat attaccagac actgacacag atgctccagt cattaatatt acaagtacta
    21961 tacatataaa tctaacaaag ttagtttttt gtcctaatcc tattgccact tcttctccaa
    22021 gacttaatat agttattctt ggagccataa taatagctaa aataaatcca ataccaccag
    22081 ctacaaatag caattttata ccttcccact tcactcctac aagccctcct gatatccaga
    22141 aacttaattg ttgagatagg ttaaaataca tagatattgc tgatgccaaa gaaattagca
    22201 atgtaccaag agcgactcct gcaagagcta attttacatt attaactctt cctcttgatt
    22261 tagaacttat aaaatatata aataatccac ttatacttgc tccaataaat gcaaacatca
    22321 ttacactaaa acttccatgt ataagttgag gaagtttttt ttgaagtacc agtgatatcg
    22381 aaattgcgaa tgtagctcct tgtgtaattc ctataacaga tggttcagct ataggatttc
    22441 tcgttattcc ttgcattatt gccccagaaa ctgctaaaaa ccctccaaca agtgcagctg
    22501 ctattgctct tggaagtcta acatccttta ctattctcat atttattcca tcttccattt
    22561 ttatcaaact atctatgaca gttgatagat tcatatcttt tgccccaata gttatggata
    22621 atacaattcc aaatacaagt acaactatac ttactaatat caacaaataa gttatatttt
    22681 ttttactcag cttagtcaat tatcctacct ccttttccct tttaatcgtc agtataatat
    22741 catctattaa gtattttaat tttaagattt attaaaaata ggtgtgaaaa atgaatatca
    22801 ttatctcaca cctactaagt taagaattac tttgttaatt catttttaac tgattctaat
    22861 aataactctt ttccaattgg attgtatgat tgactgaaaa atggagcagc atcaagtatt
    22921 gttacattac catcttttac tgctcttatt tgagaccaga tagcactatt ttctaaatct
    22981 tttttatctg cttctgtagc aattacaact atatggtctg catctatatc tgttaaacct
    23041 tccatactta ccattggtag tgttattcca tcctgttttg gcatattctt aggtctagct
    23101 aatttcatat catcattgat tagagtacct attccaccat cactaaatac cataaattga
    23161 cctgaacttg ccaacactgg taaataagtt ttttctccat tcttttctat aacttcctta
    23221 cctaactttg tagctttttc atcatacttc tgtagccaac ttttagcttc ttcttctttg
    23281 tcaaataact ttgaaacatc agttaacttg cttctccagt catttgcata atctttcatc
    23341 ataactacag gagctatttc ttttaattgg tcatatattt tttcttgtct ttgactcatt
    23401 attattaaat caggatttac ttctaatata gcttccatat ccatagtatc catcattgag
    23461 tgcccaacta tctttacacc tttaagctct tctcttatgt aacttggtaa tttatcagtt
    23521 tcatatgagt ctacatttgc tgttgcaaca ggcttataac ctgctaataa tagttcttca
    23581 ctacttcctg atatatcaac tattttttta ggatttgatg gtatcttaac ctctccttta
    23641 acagattgta caactcttgt ctctgattta tctttatcct ctgtattctt atctgaacat
    23701 gctgtaacaa gtactaacgt agttataata gatataaata ttgcaagtga ttttattttt
    23761 ttcatgatta cttctcctac ttgtttatta tacttacatg atattgatta tcataagcac
    23821 atatttatta tagcattttt ctatttttag tcaatattat tttgtaaaag gataaaaatt
    23881 cctaattaat cttaattata tatagcttag ataaggtttt cttatattaa acagtatttt
    23941 ttttaagaat tggacacaaa cacaatgact tttaggttaa acaatgataa aatgcaattt
    24001 atagataatg aatttagtaa ggaggattca aaacatggta catttagtat attgtgataa
    24061 tgcaggaaaa aaaggagaaa aggtcttaga taaaatcttg gctggaacaa aaacaatggt
    24121 tattagaggt gctgctggta gaaaaatacc acatagtaga gtatttgaag atgagttact
    24181 ttattttatg gaaaaaggaa ctggaaaaat atctgcaaaa gccactgtta aaaaagtaga
    24241 aaactatgtt aaattaacag aagatgaaat ttctaaaatt ttaaatgaaa atcaaccaaa
    24301 attaaattta tctgaaaagc aaaaagttcg ttggcataaa aaatgtttat gcttagttga
    24361 atttgaaaat gttgaagaaa taacacctct tgattttgac catcaaggaa atatggatga
    24421 ttggcttatt gttgaaaaaa ttgaagatgt agttgttggt actagtatac cttacaatta
    24481 tgagaagtct aaattttaaa gtatcaataa cataatacta tattgctaaa ctctacttaa
    24541 ataaaaaaag agtgcattta ataattataa aaattatttt tatacactct tttttaataa
    24601 taaatttaaa ttacttactt tctaatcttt catatacatc tataaattct tctgctaatt
    24661 gttgaaaatt catagctgtc attaaatggt cttgtgcatg aataagtata acacttattt
    24721 cagttttatt accattagct tcattttgta tcaattctgt ttggaattta tgagctttat
    24781 gaattgattc tttcgacttt tcaatacatt ctctagcttt atctatattc ccgctctttg
    24841 cttcttttat tgcttcaaat gctagccctt tcgcatcacc tgcaaatcca attattccaa
    24901 aagatatctc tatcatcttc tcatccattt attcgacctc ctaaatatct ctatagcttg
    24961 atatgactaa tccattcttt tctaaaaatt ctttgacttt tttagatgta agtatcttgt
    25021 gctctttcgt tctatcaata gcataagatg tagaatttaa aatataatca tctaaaaaag
    25081 ctgggtgaga cataatatcc actacatcat acttcattat ttcgtctata ttttttataa
    25141 aatattcttc acttacattt tccttataaa aactatctat taaaggaact actttagaat
    25201 actctaaatt atattcaaat ccacctctta ttggaagatt atacttggtt acaatcttct
    25261 ctactactgg ctttaaactt actaaagtat ggatatgatg gtgactatct aaatgagata
    25321 tatctattcc aagccctttt accctatcta tttgagcaca aagttctcta taaatctcat
    25381 cacaatccat tttttcaata atttcgtctg taatcctcct aataaaaaaa ccgtctttat
    25441 caacaatagt ttttaagtta gataataatg gtctaccaca acttaaagtc atatgaactc
    25501 cacaatttaa agttttattt tctttaagta agcctactcc atgttctacc ccaggcatat
    25561 ttgccatcat agaagtagac ctaactatac cattattatg agctgaaatt attccatagt
    25621 tcacagcttc acaatatcca aaatcatctg catttattat aattttagtc ataccataac
    25681 ctactttcta tagacaacta tctattaaat ctaggaagat aatctttatg agcttccaac
    25741 atttcgtcta atatagtttt tgctaagtca tcagatggaa tcaatgggtt tatacaaagt
    25801 gccaaaagag cagattcata atcgccatat acagctgctt tagctaccaa tacttcaaat
    25861 gatttgattt ggcttactaa tccatgcaca gaatctggta aatatcctat acttattggt
    25921 tttggaccat ttttggttat tacactactc acttctaccg cttgttcatc tttaaaatct
    25981 cttatagaac cattgtttaa tgtattaact acttggatat cttttttgtc attgtatata
    26041 gaacttatta aattacaagc agcatcacta tagtaagccc ctcctctttt ttcaagttga
    26101 ggtggtttta catctaattt ttcatcctta tataattcaa ataattgttc ttccaattct
    26161 ttaactactt gacctctagc ttttccctct ttaaactctc tcaactcatc ctcaagcatt
    26221 tctttagttt tatagtagta tctatgatat ggacaaggta ttgcacccaa agattttaca
    26281 aattcagcat taaaatcaat tgcctttata ttttgcatac ttatatctgc tttgacaaac
    26341 ttatcaattg cttcttttgt aacatcttct ccatctaaag atacatttag accataaacc
    26401 atatgattaa gacctgcaaa atccatactt attctgtcac tttctacatt aaatagtttg
    26461 gcaacatcat tttttaaatg aattggcaca ttgcatagac ctatatatct tctaaaatta
    26521 gtatatttga atacagcctc tgttattatt cctgttggat ttgtaaaatt caccatccat
    26581 gcatttggac ataattcttc cacatctttt attatatcaa atataactgg tattgtgcga
    26641 agggctttaa ataaacctcc agcaccatta gtttcctgcc ccataactcc atgagataaa
    26701 ggaatagttt catcttttat tctcgcatct aaaagaccta ctctaagttg tgttgtcaca
    26761 aagtctgcat ctcttagagc ttctcttcta tctaaagtaa gatttatttt catatctact
    26821 ccagcttttt ttaccatcct cttagctaga tttccaacta tctctaattt ttcttttcca
    26881 gcttctatat ctacaagcca taattccttt acaggtaact cttcatatct atttataaaa
    26941 ccctctacaa gctcaggagt ataacttgaa cctcctccaa ttgtaactat ttttagtccc
    27001 tttttcatct cattatacca tccttaccta taatttaata tactctacaa aaccctatac
    27061 atttatttac cgaaataaaa ctacctagtt gttaattttc aatttattag cttccatttt
    27121 ttcagacgct atgacaaatg gaatatatat taatattgat aatattaaat taaatagtgc
    27181 taaagctcca ccagtccagt cacctgtagc catcgctcca cctaatatag gaggtgttac
    27241 ccaaggtatt ttaggtaaaa ttactggtgc tactaatcca aaatctattg ctaagtatga
    27301 tacagcacta catatcactg gtgcaagtac aaatggaatc ataaatatag gatttaaaac
    27361 tattggaagt ccgaatatca atggttctcc tatattaaat attgcaggtg gcccacctaa
    27421 tactatcatt tctttacgtc ttctcccagc tattaataaa gctactatta atcccaatac
    27481 cattccagaa ccacctaaat atacaaatgc atcaaagaat gcacctgcaa gtacatggta
    27541 tccttccgtt gctcctgctt gagccaaggc agcattttct ccacctaatt tcataagtat
    27601 agtttctgta aaaggaagtg ctatatttgc accatgaagt ccaacagtcc aaagtatatg
    27661 aattataaat gcaataagca ttgtagaacc tagactatca gctacatttg atagcggtgc
    27721 tcctaaataa gtatttaaaa tatctgttaa gaaacttcca ttagaaagca tttttataaa
    27781 tattcctatt acagtaaata tcattatagt taacattcct ggtattaact ttgcaaatga
    27841 tcttgaaact gctggtggaa ctccatctgg catttttata gttaatttct ttaccttaga
    27901 taatcttaca aatatctcag ttgataataa tcctattaat atagaagaga ataaagctgc
    27961 tgtacctaag taggtttgtt gtatcattcc ccatccttca actactgtag ttcctccctc
    28021 aggaactatt ttaccaattt gtggagccat tataaaaaag atagataaag ctataagccc
    28081 agattgtaac ccatcactct cataagattt tgctagaaaa taagcaacac ctattactaa
    28141 gaatactgcc attgtaccta ttgctcccca ccaaaggtcg cctccaaatg tggtccaatt
    28201 ttctccaaat atacttgcca tcaaattttt gtatgcttcc aatggtaagt tatttatcaa
    28261 tgttcccaag gcacctacca tagttatagg tatcattact ataaatgcat ctcttactgc
    28321 tactaagtgc ctttgagctc ctattttagc agctacaggc actatatatt tatccataaa
    28381 tgacatgaat ttttccattt tacatcctcc agtacaactt tattttgaac tatttaactc
    28441 tacagctcta tctaacacag cttttccatt acatcttcca taatccatca tatttataac
    28501 ttctactgga atattatatg gttttgccat ttcagttgct tttcctaata catatctaac
    28561 ttgaggccct aaaagtaaga catcacaatt ttctatttca ttttttaagt tcacttctga
    28621 tatagcccat atttttgctt ctataccgtt ttcctttgct gcatcctcca tttttgtaac
    28681 taataaacta gttgacattc ctgctgaaca agctaacatt atttttatca ttttttcccc
    28741 tccaaattag attcattaaa tattttttaa gtcataaatt ttttaaacta tacatttaaa
    28801 atatgctttt ctattaattt tttcttaatg tatatatctt atttttaaat gtatatacat
    28861 ttaattcttg atattattat ataccaaatg tatatacatt tcaagaaaac tttttcattt
    28921 tatttaaatt ttattcgaca acagcattca aagttaatgt atatccatag tgatatgtgt
    28981 gtgagtactc aaaaatctca ctattttgta acttacaaat ttggcttata tataaaagtg
    29041 gctctggttc ttctaatttt agattatcca tacattcttc attcgatatt acagcttgta
    29101 cttctctctc tgacatggct atcttgtatc cacacacttc ttcaacaaaa ttataaagag
    29161 aattttcaca atgagtctta tttaaatttg gaaataactt aactggcata taagtaatca
    29221 tatatgaaac tcttgcatca ttggctaatc ttactctttt tatttcccaa acattttctt
    29281 ctaaacttat attcaactta cttgataaat tttcatctgc ctgaactatt tcaagagata
    29341 ccaatatatt agatacttta tagcctttac tttccatctc tttagtaaat ccacttacag
    29401 acgatatatt atcatatata ttatttgcaa taactatact acctacacct ctttgtctag
    29461 ttatatagcc ttctttaact agattattaa gtgcagacct tacagtcatt ctagttacat
    29521 taaattcttc actcagctgt ttttcagaag ggattaaatc cccttctttc aaactgtctg
    29581 aatttattaa ctctctaaca tgattttcta taactttata aattggctct ttcatttaat
    29641 tcactcctta ttaattaaat cttttatata ataagataat tttatataca aataaagaaa
    29701 atgtctatac atttaagata atttaaattt tattcagaca tcttgtacaa ctttccgagt
    29761 tttaataaag atataaatag acttatcatt aatatacctt ttaatatact tgataaggtt
    29821 atactccacc atacaccatt tagacccaac atttctggtc tagatataaa gtaagccaat
    29881 ggtactcttg caccagtcag tatagtaatt ataatagaag gtatataggt tctgcctaat
    29941 cctttgaaag ctcctgttgt tattatttct aaacacataa aaaattgaga atagcctaat
    30001 attttaagat aaattgctcc tttttctatt gcctcacttt catctataaa aatagaaaat
    30061 atactctctc ctgcaaatac taataccaaa gtagttatta tcccaagtac agaagataca
    30121 gttatagcta ttttgcaacc tttatttatt ctagaatact tcttggcacc ataattttgt
    30181 ccaacaaaac tgcctaatgc aactgatagc ccttcagccg tcatccagga aattgcttct
    30241 atttgtgagc ctactttttg tactgcaaca gccacaggcc cccatgatgc tactattact
    30301 cctaatagca ttgagaatac tgtaaacata ccactttgta ttgctatagg taatcccaat
    30361 ttatacagaa ctttataata tttagtctct atttctttaa atagtcttaa tttaaaatat
    30421 tcttctttac ttttaactat tatatatata aaacatgtag taactactat ctgagcaaat
    30481 acagttgcaa tagcagctcc tgcaacacca agttttggag caaatcccca tccaaaaatt
    30541 aatataggat caagtataat attagttatt aatcctactg tattaatacg aaaaggagtt
    30601 ttactatttc caagaccgtt gaaaattgca gtaaatactg gattaataaa ataaaatacc
    30661 atacctagag ctactattat caaatactgt ctagacatgg ttataacttc taagtctcct
    30721 aatctaaaaa aacctatcaa ttgtttatta agtactatta aagaaattgt atatattatt
    30781 gctagtataa tatttatttc tattgctgac ttcatatatg atttagtagt gcttatatta
    30841 tttgcaccaa tactttgtgc tacttttatc tctccaccta cttttgaaat cataataaat
    30901 gccattgcaa gccatggaaa aaaaccagct gttccaactg cagctactgc tttacttccg
    30961 gctttaccaa cccatatcat atctatcatg ttatacccca tctgtataaa tgaagtcccc
    31021 attattggaa gtgatagctt aagtaatttt tctgtaattc ttccttctgt taaattaatt
    31081 ctgtctttca ttttccacct cttttatatt aaatttattt gcttatatag ctagattaat
    31141 cattttattt gattgagttc actatccttc aatttatatt atcatatctc aagattgcat
    31201 ctcaaccata cttatgttta agataaagta ttttacctat ctctgataaa attaatactt
    31261 atgaatataa atatagtcat ttcatcaata catattataa cccttaactt aacttcaatt
    31321 aagtatacat actgtaatca attagaatat tttataatgt atcttccatt aagaaataac
    31381 aaatataagt gatttatcta taattttatt tatacataaa atagtatact gcaataataa
    31441 aaaaactata tttgaatact ttaagcatgt ttactaattg taatttccac acaaatttga
    31501 taatatatga atatattatg gtgaaattta tagatatata ttaattatgg ggagattgtg
    31561 ggaggttctt ttcacaagac atttaagcta tgaaaataaa taaaaaattt aatagagttg
    31621 atatgcaggt ttcaatactt actgcaatta ttgtaataat atcttcctca tgtgtatatt
    31681 tttttaatta tacattaact tataagagca tgatatactc tttaagtgag aggtcaaaac
    31741 atatctatga atatgtagag agaaatattg ataaaaatac ctttactgaa attaattcta
    31801 aagaagacca atcaaaaaag tcttataaaa gttctaaaat cctattagaa tctgtaaaaa
    31861 atactactgg agtcatgtat ctatatactg ctaaaaaaac aaaagatgga tcactagtat
    31921 atgttgttga tggtttaaat tcatctagaa gtgattttag aaatgctggt gatttgatag
    31981 aaaaagagat ttggggagat cttaacaaag cattagataa tcatatattg ttacctaaaa
    32041 agattaaatc aacatcttgg ggatacatat ttatatcgta ttttcctatt cacaatgatg
    32101 ttggtaaggt tgttggagta ataggaattg aatttgaagc aaaacatcaa tacaatacat
    32161 ttaaatttat aagtatagtt acaccaataa tagcattatt aacttgtcta atatcagcat
    32221 tgattgcagt gattctattt aaaagaatat ctaatcctac atatactgat tttgctaata
    32281 cagattattt aacaggccta aaaaatagaa atgcttttga aatagatatg aataacttaa
    32341 acaattcaaa tctaaagaat ctaatatcta tcatatcaat agatttagac ggtttaaaaa
    32401 agattaatga taaatttggc catcagacag gagacttata tattaagcaa gcaagtaaaa
    32461 ttatacaaga tgtaataaat aaaaaatata tagtttatcg tattggaggt gatgaataca
    32521 t
//
LOCUS       DACTGW010000017        32295 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.017, whole genome shotgun sequence.
ACCESSION   DACTGW010000017 DACTGW010000000
VERSION     DACTGW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.017"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            32..1609
                     /locus_tag="I9573_000651"
     CDS             32..1609
                     /locus_tag="I9573_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HAT4795285.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVENLEAKVDSIEKAIDSISQKVN
                     KIQDILDKIGFLKKFLS"
     gene            1743..3080
                     /locus_tag="I9573_000652"
     CDS             1743..3080
                     /locus_tag="I9573_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HAT4795286.1"
                     /translation="MGIGIECNKKINKSIDKTRIKKNNLNSNSNSNSNSNSNSNSNSN
                     SNSNSNSNSNSNSNSNSNSNKISNNVVDSKLTNSRIKSIDIIRGLSIALMIVCNNPGT
                     WMRMYPQLRHAVWHGVTLADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKR
                     SILLILFGFFLNYLGNPDLNSVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIIT
                     FLCIATFIIVGYYILAKPYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAIS
                     SGMLGCTMGCVLLKENIGEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLL
                     MAGSYGMLLSIFYFICDIKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNK
                     VDYPTTLVEWTTYELITPWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            3334..5358
                     /locus_tag="I9573_000653"
     CDS             3334..5358
                     /locus_tag="I9573_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT4795287.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAGT
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            5608..6264
                     /locus_tag="I9573_000654"
     CDS             5608..6264
                     /locus_tag="I9573_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAT4795288.1"
                     /translation="MPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQRIMDLVLSIIG
                     LVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTDAEKFGAQWAE
                     KDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQFNEEIPGFINR
                     LAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTVKVVLTGDGAR
                     "
     gene            6359..7414
                     /locus_tag="I9573_000655"
     CDS             6359..7414
                     /locus_tag="I9573_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HAT4795289.1"
                     /translation="MIKKISTILSLVLLISISSTIEVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGIKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTAKLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            7529..9088
                     /gene="murJ"
                     /locus_tag="I9573_000656"
     CDS             7529..9088
                     /gene="murJ"
                     /locus_tag="I9573_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAT4795290.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDDKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRRR"
     gene            9130..10836
                     /locus_tag="I9573_000657"
     CDS             9130..10836
                     /locus_tag="I9573_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAT4795291.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNDTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            10858..11931
                     /locus_tag="I9573_000658"
     CDS             10858..11931
                     /locus_tag="I9573_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HAT4795292.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEYISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            11943..12665
                     /locus_tag="I9573_000659"
     CDS             11943..12665
                     /locus_tag="I9573_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAT4795293.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            12681..13838
                     /locus_tag="I9573_000660"
     CDS             12681..13838
                     /locus_tag="I9573_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HAT4795294.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNIDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTIFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            13841..14680
                     /locus_tag="I9573_000661"
     CDS             13841..14680
                     /locus_tag="I9573_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT4795295.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNEDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYTFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            14695..15870
                     /locus_tag="I9573_000662"
     CDS             14695..15870
                     /locus_tag="I9573_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphotransferase"
                     /protein_id="HAT4795296.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            15899..16648
                     /locus_tag="I9573_000663"
     CDS             15899..16648
                     /locus_tag="I9573_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT4795297.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYKRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            16670..17578
                     /locus_tag="I9573_000664"
     CDS             16670..17578
                     /locus_tag="I9573_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT4795298.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            17626..18378
                     /locus_tag="I9573_000665"
     CDS             17626..18378
                     /locus_tag="I9573_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT4795299.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            18404..19699
                     /locus_tag="I9573_000666"
     CDS             18404..19699
                     /locus_tag="I9573_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HAT4795300.1"
                     /translation="MKIAIAGTGYVGLVTGVCLAEVGNENVVCVDVDEQKVKSMKLGI
                     APIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVGTPERSDGSANLDYIKA
                     VAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNNVKVELASNPEFLSQGTAV
                     KDTLYASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAEMIKYASNNFLALKISF
                     MNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGSCFPKDTKALHWLSEHN
                     GYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGLTFKPDTDDIREAPSIP
                     NIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIEDAEICFIMTEWKDVLN
                     YDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            19705..20796
                     /locus_tag="I9573_000667"
     CDS             19705..20796
                     /locus_tag="I9573_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAT4795301.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            20880..22793
                     /locus_tag="I9573_000668"
     CDS             20880..22793
                     /locus_tag="I9573_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAT4795302.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNSELKKVAVAIIDDDKDKIGRIIHNVKIVGTTSDIKAVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            22992..23699
                     /locus_tag="I9573_000669"
     CDS             22992..23699
                     /locus_tag="I9573_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAT4795303.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            24001..26112
                     /locus_tag="I9573_000670"
     CDS             24001..26112
                     /locus_tag="I9573_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HAT4795304.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTAL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(26310..27275)
                     /locus_tag="I9573_000671"
     CDS             complement(26310..27275)
                     /locus_tag="I9573_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAT4795305.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(27560..28516)
                     /locus_tag="I9573_000672"
     CDS             complement(27560..28516)
                     /locus_tag="I9573_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAT4795306.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVDIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            28884..29672
                     /locus_tag="I9573_000673"
     CDS             28884..29672
                     /locus_tag="I9573_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAT4795307.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVDDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(29795..30265)
                     /locus_tag="I9573_000674"
     CDS             complement(29795..30265)
                     /locus_tag="I9573_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HAT4795308.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            30404..31048
                     /gene="uppS"
                     /locus_tag="I9573_000675"
     CDS             30404..31048
                     /gene="uppS"
                     /locus_tag="I9573_000675"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HAT4795309.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            31091..31996
                     /locus_tag="I9573_000676"
     CDS             31091..31996
                     /locus_tag="I9573_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT4795310.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQKVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        12847 a         3502 c         5758 g        10188 t
ORIGIN      
        1 aagattttaa acaaaataag ggggaataat aatgaaagca ccaaaaacta ttttaacaat
       61 actgacaata gcgcttactt taagtagcat ttctataata ccatcatatg cacttacaga
      121 ggaaaaatta ataggtaatg gtagatatga aactgctgta aagataagtc aaaaggcata
      181 cagttctagt tctaatgtag tgttagttaa tgacaattca ttagctgatg cactttcagc
      241 tacaccattt gcaaaggcta aaggagcacc aatacttttg acagaaagtg ataagctaga
      301 tgataggact gaaaaagaga taaaacgtct aggagctaaa gatatttatt tgataggagg
      361 tactgctgta cttaataaag atatagagaa caagttaaaa ggtaatggac ttaatgtaga
      421 aagaattaat ggtaaggata gatatgaaac ttcactgatt ttggctaata agcttaagga
      481 tataaaagat attaaggaag ttgctgtagt aaatggtgaa aaaggattaa gtgatgctgt
      541 tagtgttgga gcaccagcag ctcaaaataa gatgcctata attttgtcta gtcctaagga
      601 cggagtagaa gcatttgata aatttataag agatgagaaa gttataaaag catacgtaat
      661 tggaggaaca aattctgttt caagagctgt agaaaagagc cttcctaatg cagaaagagt
      721 gagtggaaaa gatagaaatg agactaatgc caaagttata gaaaagtttt atactgatac
      781 aaatttaagt aatttatatg taactaagga tggtagtaaa aatgagaatc aactaataga
      841 ctcgttagct gttggagtat tggctgcaaa aaatgagtct cctgttgttt tggttggtaa
      901 taaattaaac acgaaacaaa gggatatact tagtactaaa aaattaaata ctataacaca
      961 ggtaggtgga aatggaaatg aagaagcttt tgatgaaatt aagagtttac aagaaaaaac
     1021 tgtgtttgaa gcaaaaactg ttgaggaatt aactgatatg ataaatatag caagtcctaa
     1081 tgatattata aactttaaac caaaagagaa tactgtaaat gaagctttta gaatggtaac
     1141 aaataaacca ataactgtga atataaaggg tgattgttca aagactctta cagtagatat
     1201 gcctaatggt gaagtgaata actatgctac tttggtaaat gttatagtta gaaatattgg
     1261 agaaggtgga tttaataacc atgatacaat cactatattg tcagttcgtg acaaaaatgg
     1321 tagagttata gaaaatacta gaaattcaga tatagatacg ttgatgatat tagcttctgc
     1381 taatgataca aaattgatta atgatggata tataggaaag cttatagata attcatctaa
     1441 tagtgatata actaataatg gtactataga taaaaaagtt aatcaagttg agaatttaga
     1501 ggctaaagta gattcaattg aaaaagctat agattctata agtcaaaaag tcaataaaat
     1561 acaagatata ctagataaga ttggatttct aaagaaattt cttagttaag aatatgtata
     1621 tttaagtgaa gtgtgtgttg taattgttct aaagttatat agttaaacag gtttatatac
     1681 aaacagactt atagataaat atgtagttta gtatatattt gaataagctt tggggaggta
     1741 acatggggat tggaattgaa tgtaacaaaa aaataaataa aagtattgac aaaactagaa
     1801 taaagaaaaa taatctaaat agcaatagca atagcaatag caatagcaat agcaatagca
     1861 atagcaatag caatagcaat agcaatagca atagcaatag caatagcaat agcaatagca
     1921 atagcaatag caataaaatt tcaaataatg ttgtagattc taaacttacg aatagtagga
     1981 ttaaatcaat agatataata agagggctat caatagccct catgatagtc tgcaataatc
     2041 ctgggacttg gatgagaatg tatcctcaat taagacatgc tgtatggcat ggtgtaacac
     2101 ttgcggattt tgcatttcct ttttttgtta tatctttagg tgttacaatt ccaatatcaa
     2161 taaatagtaa actaaaaaat aataaatcca cactgagtat aatacttagt atattcaaga
     2221 gaagtatctt attgatatta tttggatttt tcttaaatta cctaggaaat cctgatttaa
     2281 attctgtgag aatacttgga gtacttcaac gtatgggatt agtctatttt gtgactagtt
     2341 tagtatattt actactaaaa aaattaaatg ttggaagtac tgctacaata attacttttc
     2401 tatgtatagc tacatttata atagttgggt actacatatt agcaaaacct tatggatttg
     2461 agctagaagg ttctcttgca cagttagtgg atttacattt cttcaaggga catttgtaca
     2521 agccagaatt tgaaccagat ggttttttaa ctagtatagt agcaatttct tcaggtatgt
     2581 tgggatgcac tatgggatgt gtacttttaa aagaaaacat aggagaatat aaaaaattct
     2641 ttaaaatact ggttatgagc ataatcctgc taataggtgc ttttatcttt aatcagtatt
     2701 ttccatttaa taaaagatta tggtctagct catttgtact tttaatggct ggcagttatg
     2761 gtatgttact gtcaatattt tattttatat gtgacatcaa aaataaaagt aaaatattta
     2821 ccccaataat agcacttgga agtagcccta tttttacata tatgtgcctt gagatattga
     2881 gtcatgtttt ttggaatgtt ccaaaactta cgaacaaagt cgattatcca actaccttgg
     2941 tagagtggac tacatatgaa cttataacac cttgggcagg tacaacttgg gattctctta
     3001 tattttcttt attgtatgtc ctattctggg ttatagtaat gtctataatg tacaagaaaa
     3061 aaatatttat taaaatttag tgcaatattt ttagatgata aagtctttga tttgttcaaa
     3121 tatacggaaa ctatcaaata taatcaaaat ttctcctcga taaaatttaa aaaccatgcc
     3181 ataaaagatt acaaaaagtt tacaatttgt aattttttgt gctataatga aaaaagattt
     3241 acataatatt gaatttatgt aatataaaac aagcttaaga tgtattaaaa aagatttaaa
     3301 gatttaaaga accaaaaaat taggggggat atgatgaaaa aaacaacaaa attattggca
     3361 acaggaatgt tgtctgtagc aatggtggca ccaaacgtag ctttagcggc tgaaaatact
     3421 actgcaaata cagaatctaa ttcagatatt aatataaatt tacaaagaaa aagtgtagtg
     3481 ttaggaagta aatctaatgc aagtgtaaaa tttaaagaaa aattaaatgc tgattcaata
     3541 acattaaatt ttatgtgtta tgatatgcca cttgaagcta ctttaaatta caatgaaaag
     3601 acagattcat atgaaggtgt aataaactat aacaaagacc cagaatactt aaatgtatgg
     3661 gaacttcaaa gcataaaaat aaatggtaaa gatgaacaga aagttttaaa caaagaagac
     3721 ttagagtcta tgggattaaa tcttaaggat tatgatgtta cacaagagtt tataataagt
     3781 gatgcaaatt caacaaaagc agtaaatgaa tatatgagaa aaacttctgc gccagtaaaa
     3841 aaacttgctg gagctactag atttgaaact gctgttgaga taagtaaaca aggttggaaa
     3901 gatgggtcta gtaaggttgt aatagttaat ggagaattag cagcagatgg aattacagct
     3961 actccacttg catctacata tgatgcacca atattacttg caaataaaga tgatatacca
     4021 gaaagtacaa aagcagaatt aaaacgtcta aatccaagtg atgtaataat tataggtgat
     4081 gatggttcag tttcacaaaa ggcagtatct caaattaaat ctgctgtaaa tgtaaatgta
     4141 actcgtattg gtggagttga tagacatgaa acatctttac ttatagcaaa agaaatagat
     4201 aagtatcatg atgttaataa aatatacata gcaaatggat atgctggtga atatgatgct
     4261 cttaatattt catcaaaagc tggtgaagac caacaaccta taatattagc taataaagac
     4321 tctgtaccac aaggtacata taattggtta tcttcacaag gtttagaaga agcttattac
     4381 ataggtggta gtcaaagttt atcatcaaaa attatagacc aaatatctaa aatagcaaaa
     4441 aatggtactt caaaaaatag agtttctgga gcagatagac atgaaacaaa tgcaaatgta
     4501 attaagactt tctatccaga taaagagttg agtgcaatgt tagttgcaaa atctgacata
     4561 atagttgact caataacagc aggaccttta gcagctaaat taaaagctcc aatacttata
     4621 actccaaaga cttatgtttc tgcataccat agcacaaact tatcagaaaa aacagcagga
     4681 acagtatatc aaattggtga tgggatgaaa gatagtgtta taaattctat agcatctagt
     4741 ttatcaaaac acaatgcacc aacagaacca gataattcgg gttcagcagc aggaaaaact
     4801 gtagtgatag acccaggtca tgggggttct gattcagggg caacaagtgg attaaatggt
     4861 ggagcacaag aaaagaaata tactcttaat acagcattag caactactga atatttacgt
     4921 tcaaaaggta taaatgttgt tatgactaga gatacagaca aaacaatggc tttaggagaa
     4981 agaacagcat tatcaaacac tataaagcca gatttattta caagtataca ttacaatgcg
     5041 tctaatggag ctggtaatgg tgtggaaatt tattacaaag taaaagataa aaatggtgga
     5101 acaactaaga ctgcagcatc aaatatctta aaaagaatac tagaaaaatt taacatgaaa
     5161 aatagaggaa tcaagacaag aacacttgat aatggaaaag attacctata tgtgttaaga
     5221 aataacaatt atccagcaat acttgttgaa tgtgcattta ttgacaataa gagcgatatg
     5281 gataagttaa atactgcaga aaaagtaaaa acaatgggaa ctcaaattgg aataggaata
     5341 gaagatacag taaaataaaa taaaaaatat taaagcaaaa catatcatct cctgttataa
     5401 tgtaaaatat aacaagagat gatatatttt tgtaaagaat caaattgttt acaatgatgt
     5461 ttcttgtatt gataatctgc tcattattga gtataatata gaaataagtc aaaaataaga
     5521 tagaaataag ttgaaattaa gaattaaaaa aatttttatt ccaatatgga ggtaactatg
     5581 tctggatata ctaatgatga gtgtgaaata cctaaaatta aaagctatcc aggggcagat
     5641 aaagaaattg caagtgagat tgactatagc atagtaaaag gaacggtttt attcgacttg
     5701 taccaaagaa ttatggattt agttttatct ataataggac ttgtgatagg tttaccttta
     5761 atagcaatat ttggaattct tattaaaata gaggacaaag gaccaataac ttataagcaa
     5821 gaaagactag gaaaatgcgg gagaagattc tatatatata aattaagatc tatgagaaca
     5881 gacgcagaaa aatttggcgc acaatgggct gaaaaagatg atcctagaat aactaaggtt
     5941 ggaaagttta ttaggaaaac tagaattgat gagattccac agctgtttaa tatattaaag
     6001 ggggatatgg gcttaatagg ccctagacca gagagaccga actttactgt tcaattcaat
     6061 gaggaaatac ctggatttat caacaggctc gctataaaac ctgggcttac aggttgggca
     6121 caagtaaatg gtggatacga aatcactcct gaagaaaaac ttaaagaaga tatttactat
     6181 ataaaaaata ggagtatatt acttgacttt aagatactct ttaaaactgt taaagtagta
     6241 ttgacaggag acggagctag ataattttaa aatattagat tactaatcaa tgaaaaagct
     6301 ctttaaaaag agctttttta ttgtcaaaaa atacaaaaaa gaaaaggaat gagaacttat
     6361 gattaaaaaa atttctacta tattatcgtt agttttgtta atctctattt ctagcacaat
     6421 agaggtattt gcagatgcaa atcccaaaag agaattgata gaagggagta taccagagat
     6481 ttcgacagaa ttaaataaaa gagcatttaa ggattctaaa gaagtaattc ttgtaaatga
     6541 agagtcaatt gtagatagta taagtgcaac acctttagca tatgcaaaaa atgctccaat
     6601 agttgtaact aagagtaaaa atcttggtag agtaacaaga aattatctaa aagaactagg
     6661 tccagagaag gttacgatag taggtggttt aaaggcagta tctaaagatg ctgaacgtaa
     6721 tatagaaaaa atgggaataa aagtagaaag aataagagga aaagacagat atgatacatc
     6781 tttaaagata gctagagaaa tgtatagaac agtaggattt gatgaagcat ttttacttag
     6841 ctcaacaaca ggattagaga atgccatatc tgtatattct tatgccgcta aaagtggaat
     6901 gccaataata tgggctaaag atgaagggtt tgaggaacaa attgatttct taaaaggtaa
     6961 gaatcttaaa aaaatatatg cattaggtga ttcaaaagaa tttattgcag agattgattc
     7021 taacttgaaa aatatagaag gaataaagca aataaataag tcaagtacaa atgttgattt
     7081 aataaagaag ttttatgatg aaaaagatat aaagaaaatc tatactgcta aattagactt
     7141 tggtagcaga tctgatgtaa acgaatatat ttctttggga gtagtatcag caaaagaaaa
     7201 tatgccaata ctgatttgta gtgataactt aagtcgtgca caagataaat tcttgaaaga
     7261 tagcaatata aatgatgtag ttgaagttgg gtatacagta ggtgattata gtctatttaa
     7321 gagcatattt aatctaacat tcttatcttg tatagtatta atactattat tactattgat
     7381 tacatttagg gcacttagat atgagtcgaa atagttttta atttataagg aagcttttca
     7441 taggttaaat ttacttaatt agaataatgt ttataagcag ttttaaaagt tctactttta
     7501 gagtttaaat tagataagga gaaagaagat gtcaaaaaca gctaaagcag cattgtggat
     7561 tatggcagcc actatgtttt caaaagtatt aggtttcctt agagaacttg tacttgcaaa
     7621 tttctatggg acaggtatgt atgcagacgt atttgtatta acattaaata tacctggttt
     7681 aatcatagca gtcataggct ctgcagttgc tacaacttat atacctatgt actttgagac
     7741 aaagaaaaga ctcggagatg aaggtgcctt aaaatttact aataatgttt taaatatatg
     7801 ctatataatg gctatagtaa tagctattat tgggctttta tttacagagc aatttgttac
     7861 agtatttgca gcaggattta gaaacgaccc tgctaagttc caagcagcaa tattatttac
     7921 taaaataatg atttcaggag ttttattcct aagtggaagt aagatattta gttcgttttt
     7981 acaggtgaat gacagttttg taattcctgg gcttatagga atcccatata atattataat
     8041 aatagcagca atagcactaa gtgcaggtaa aaacgtatgg atactaccag caggagcact
     8101 attggctatg gcgagtcaac tattatttca actgccattt gcatttaaga agtcctacaa
     8161 gtataagcca tatataaact taaaggatga gtctataaaa gaattagtaa acctagtatt
     8221 gcctatgttg gtaggagtag ctgttggaca gttaaatata tttgttgaca gattattggc
     8281 aactactttg ggtgatggaa agttgtctgc tcttaactat gcaaataggc taaatgagtt
     8341 tgttatggca ttatttgtta catctataat aacagttata tatcctaagc tagcaaaaat
     8401 gtctggtaaa gatgataaag aaggatttat aagtactatt gtaaaatctt caaattgtat
     8461 tatattggtg gtgcttccta tatctatagg agctattata ttagcagagc ctcttgttag
     8521 gatattattt caaaggggta aatttgatgc gttatcaaca gatcttacat ctatagcgct
     8581 tagattatat tcactaggtt tattggcttg tggagtaaga gatgtcttat atagggcatt
     8641 ttactctctt tcagatacaa aaacgccaat gataaatgga agtatagctt taataataaa
     8701 tatagttctt aatttaatat tgataagacc attaggtcat gcaggtattg ctatatcaac
     8761 tagtacatcc aatataatta cagttatatt gttatttata tctcttaaaa agaaaaatgg
     8821 atattttgga ggggataaaa ttataaagac tggtctaaaa agtttggtag catctggtgt
     8881 gatggcagta gctactttat tgatatataa taatctatat gcatttatgg gttcaggaac
     8941 aattaaagaa attatatcag ttggagctgg agtattaggt ggagcttctg tatatactgt
     9001 attgatagtt ttatttaagg tagaagagat ggacttagct tttgaatttt taagaaaagg
     9061 aaaacaaaaa ctacttagaa gaagataatt accttgaaat tttaaattag agtcagaaag
     9121 gatgaaaata tggattataa gaataattat gagatgtggt taaattctcc ttattttgat
     9181 gagcaaacaa aaaatgagct attaagcata aaagatgatg aaaaagaaat acaagataga
     9241 ttctataaga atttagagtt tggaacaggt ggtttaagag gtataatagg agcgggaact
     9301 aatagaatca atatttatac tgttagacgt gcgacactgg gcgttttaaa ttatatcatg
     9361 aagactcaag gagaagaagg taaacaaaag ggaatagtta tagcacatga tagtagatac
     9421 atgtctaggg agttttgtat agaagtagca aagactttaa gtgcttatgg tgtaaaggct
     9481 tatatttttg aagaattaaa gcctacacca gaactttctt ttgctgttag atatttaaag
     9541 tgtgctatgg gaatagtaat aacagcaagt cataatccta aagaatacaa tggatataaa
     9601 gtatatgact cagatggtgg tcaaatctgt atagatatgg caaatgatat aatagcagaa
     9661 gttaataaga ttgatgatta cagcactatt aaaagtatag attttgaaga agctttatct
     9721 aaaaatttaa taactatatt agataatgaa gtagatgatg agtttataaa agctgttaag
     9781 aagcaagttt taagacaaaa tataatagat gaatacggga aaaaattaaa aataatatat
     9841 acacctattc atggtacagg aaataaacca gtaagaaaag tcttaaatga atgtggtttt
     9901 gaaaatgtaa tggtagttaa ggagcaagaa ttaccagatt ctaatttttc aacagtaaag
     9961 tatcctaatc ctgaagaaaa gtcagtgttt aatatagcta ttgaaatggc aaagaataat
    10021 gacactgatt tgataatagg aacagaccct gattgtgata gagtaggtat cgttgtaaaa
    10081 gattcaagtg gtgaatatgt agtcttaaat ggaaatcaag tcggttcact tttagttaga
    10141 tatatattag agagtttagt tgaagaaaat aaacttccta aaaacaatcc tacaataata
    10201 aaaactatag ttacatcaga actaggtgca aaaatagcaa aggcttataa tgtagattgt
    10261 ttaaatactt taacaggatt taaatttata ggagaaaaaa taaaagcgtt tgaagaaagc
    10321 aatgatagaa gttttataat gggatatgag gaaagctatg gttacttaat tggaactcat
    10381 gctagagata aagatggtgt tgtatcttca cttatgatat gtgaaatggc agcatactat
    10441 tcttcaaaag gtatgaactt gtatgaagct ttgatagata catataataa atttggatac
    10501 tacaaagagg acttaaagtc agtaacttta aaaggtatag atggaataaa aaaaataaaa
    10561 gagatgatgc tttactttag aagtgttaaa atagataatg tggctgatgt aaaagtagat
    10621 aaaattttag actataaaga tggtgttgat gatttaccta agtcagatgt acttaaattt
    10681 ttacttgaag atggctcttg gatagctata agaccttcag gtacagaacc taaaattaaa
    10741 ttttactttg gagcaaattc agacaatcaa gaagatgtag aatttaagtt gaacaattta
    10801 atatcttata tattaaatgt agtggattct atataaaaag ctaaggggag ataaataatg
    10861 aaagtataca atgtcataat ggctggagga ggaggaactc gtttctggcc acttagtaga
    10921 caagaagtac ctaaacagct tataaattta agtggagaag atgctttaat aaacgaaact
    10981 ataaacagaa tagattctct agcaaaaaaa gatgatttat ttatagtaac caatgagaag
    11041 caattggaag ctttaaagga tatagtaaaa gataaatgtt tggatagtaa tatattgcca
    11101 gagccatgtg ccagaaatac agcagcagct ataggctttg cagcatttaa cataatgaaa
    11161 aaatatggtg atggtgttat gtgtgtatat ccagctgacc attatataaa agatgaaaaa
    11221 gaatttaaat ctattttgga aaaagctatc tatatagcag aaaataatga taaactagta
    11281 actattggga taactcctac atttccatca acaggatatg gatacataaa ttttaataga
    11341 gaaaatacga tagaagatgt tgcatatgag gtagtggagt ttgtagaaaa accaaactat
    11401 gaaattgcta aagaatatgt aaactctaaa aagtatgtat ggaatagtgg aatgttcgta
    11461 tggaaggtat ctaaaatatt agaagatttt aagagatact tgccaaaagt atatgaaaaa
    11521 ttagaatata taagtaaata cttaggaaca aaagaagaaa tggaaaaaat aaaagaaata
    11581 tacccaacta tacaatcaat ctctatagat tatggtatta tggaaagaag taatgatgtt
    11641 atagtagttc ctggtgattt tggatggaat gatgttggtt cttgggattc tctaggtgca
    11701 atatatccaa ctgatgatga aggtaacatc aaaagaggtg aaaatattac tatagatact
    11761 aaaaattcaa ttatatattc agatgacaaa ttaatatcaa caataggtat tagtgatttg
    11821 atagtagtat ctacgaatga tgcagttatg gtttgtagaa aagataaagc tcaagatgtc
    11881 aaaaaaatag tagaacagtt aaaagaagaa gatagacaag aatatatgta ggaggtgttc
    11941 atttgattcc taaaaaaata cattatgtat ggtttggtgg tccaaaagga aatatagaaa
    12001 atatatgtat aaattcttgg aaagaaaagc ttccagaata tgagatagta gagtggaatg
    12061 aaaaaaactt tgatatagag aaagaaataa aaggaaataa gtttttggaa gaatgttata
    12121 aaagaaaact ttgggcattc atatctgatt atacaagaat aaaagtactc tatgaacaag
    12181 gtggagttta tatggataca gatatgcaaa tattaaagga tataactcct cttttagaaa
    12241 ataataggct aatctgtggg tatgaagatg atagagagta tataaatggt gctataatag
    12301 gtgtagaaaa aggacatcct tttttaaaag acttattgga gtattatgaa aaagaagtac
    12361 ttacttcttc attgtttaca ataccaaaaa taatgactca tcttatggaa aagaattata
    12421 agaagattga cccaaataat tatgaagaag gaatacgtgt ttacgacaaa gaatattttt
    12481 atccttttgg atttaaagaa gactttacac cagaatgtat aacagaaaac acttttggga
    12541 tacattggtg gggaaaaagt tgggctaaaa agagaaatta tttcttagaa agcaaacact
    12601 taacaggtgt aaataagata tggaagtgtt gtaaaatatt tgcaagtaat actttacgaa
    12661 gctaaaaggg agaaagtaaa atgaagactt taaatgatga tagaaatatt atgattagaa
    12721 aatatcttta tatatttgtt ttgtttatca tattacttag aaagtatatg atatcattta
    12781 ttgacccaaa tatagatata gggatgatta aatcagcgtt attttattct tcaataggaa
    12841 tactaatgct acttttttta tttgataaga gaaaaagtat aacagaaatg gtactagttg
    12901 gagtttgtgt cttactttat ttattaaata gggaaggagc tatattatta atcgttttat
    12961 tagctgtatc agctaagcaa atagatgata aatttatagt taaaaattat ttaataatat
    13021 cggcatgttt tttaatggta gctatattat tatttaattt atttccttca ttaatattta
    13081 atcaggaagt ccactataga tatatagaaa aaatagatat gcttgttaca agaatggatt
    13141 ttggacttgg taatccaaat agtgtatttt atcatatggt tactatatat gcagcatata
    13201 tattcttaag atataaagat tataataaat gggatagaat aattttattt ggttctgcat
    13261 tttttgttta tcaaaccaca tatagtagaa ctggattttt cactatatta gcaggattaa
    13321 tatttgtaga aataataaga tggatagata taaaaaaaat aaaaggactg ccaatgctcc
    13381 taaagacatt accaataata cttactctat ttagtgtaat tattggtaca atatttgata
    13441 aaagcacttt attaaataga ttattagcaa gtagacctaa gttctggcat gtatacttgg
    13501 ctgaaaaggg gaattttcta aaaccttttg gaaattcata tagtcctgct ataaaagcaa
    13561 ctaatccatt agatagttct tatgtatata taattagtgt actaggagta gttgcttgta
    13621 tattatttat gtatttgatg tataaaggaa tagaaagctt tatagaaaaa gataaaaaag
    13681 catatttagt agctttattt atatttttac tatactcttt tgcagaaaat ttattattag
    13741 aagcttcatt tagttttgca gtagtattac taataaaaga agtgatatta aatgataaaa
    13801 gggaaataga tttgtggaag atgaaaagta ggaggtagta atgttaatat cattaataat
    13861 gccaacatta aatagatatg atgatatata cttacttatg gatagcttag aaaatcaaac
    13921 ttacaaaaac tttgaactta tagtagtaga ccaaaatgat aatagcaaag ttaaagaaat
    13981 agttgataaa tacattgata agttagatat aaagtatata aaaagctcta aaaaaggatt
    14041 gagttataac agaaatgtag gcatagataa tgctgtaggg caaattatag gtttccctga
    14101 tgatgattgt gtctacgaaa atgatacatt agaaaaagtt ataaatttct ttaataaaaa
    14161 tgaagattat aaaatataca gttgcaaaac tatggattca aataaagttg atgcatttaa
    14221 gaaaatgtat gatggaactt gtgatatcac aagttccaac gttttggata ctataacatc
    14281 cataactttt tttattgatt ttgaaggtaa agattataca agatttgatg aaaaattggg
    14341 tgttggtgga gagtttggtg ctggtgaaga gatagattat gttttaaact tattgagttt
    14401 aggttttaaa ggaaaatact ttggaaatga tataatttac catcctgcaa aaaaacattc
    14461 taaatctaaa gaaaagtatc aaaaagatta taattatgga agaggctttg gagcgctttg
    14521 caaaaaggaa atagtctata gaaaaaatta tacgtttgca aaagttatgg tatcaaaact
    14581 tgtaagaaat atagggggtc ttatattgag ttccaataga gattatcata gtgccactat
    14641 aaaaggaaga attaatggat ttagacagta caagttataa tgaggatggt gtttatggac
    14701 aaggttaaat ttttaataaa tatgcttttg gcttttttta tatatccatt taataagcat
    14761 aagtttaatg gtagagatat ctggcttata ggtggacatt caggggatat atacaatgat
    14821 aattcaaagt ttttttatga gtatatgcta aaagaacata atgatgtaga aacttattgg
    14881 gtagtaaata aagacagtaa agtatttgat aaaattccag gcaaaaaatt aataagagga
    14941 agtgtagaaa actatctgta ttattacaat tcaaaagcta tagtattttc acatgcacca
    15001 tcagcagata tagcaccata caattttgct gtacctgttc ttaattattt tcataaaaaa
    15061 acgataaaag tgtttttaaa tcatgggact ataagcttta aaaaaagaaa acctatgaac
    15121 aaaaagttca aaaatattat agataaccta tataaaagct ataatatagt tacagcaagt
    15181 tctgaatttg aaagaaatgt tatggtaaat gattggggaa tgttagatga ctcagtttat
    15241 ataattggga atgcaagata tgacaattta ccaacaaatg aagttgcaca aactcgagat
    15301 atattatata ctcctacatg gagagattgg ataaaattta gttcaggaaa atttacggat
    15361 acagattatt ttaaaaatat aatgaatttt ttaaatgatg acaaattaaa taaaatatta
    15421 gatgagaaag atataaatgt aaaaatttat atgcatcatc ttatgcatga gtttatagat
    15481 gatataaaag aaaatataac aggaaaacga attgtgtttt tagataaagg ggtaactcta
    15541 gcaaatgaaa tcagaaaatc agctgcaaat ataactgatt attcaagtgt agccatagac
    15601 tttttgtata tgaatagacc tatcttgttt taccaattcg atttggatga atatatggaa
    15661 aaagttgatt catatataga tttaaaaagt gaaatgtttg ggtctttggc ttataataat
    15721 gatgaagccg tgaataaact tattgatatt attgaaaata attttgaggt tatggataac
    15781 caaaaaaatg aaagaaataa gtttttcaga tataatgata ataagaactg caagaggata
    15841 tatgattgtg tattaagtaa aattaaataa atattttata aatgatgagg aaggtaatat
    15901 gaagaagaat ttagtatcta taattactcc catgtataat tctgaaaaat ttattgaagc
    15961 aaccataaaa tcagtattaa accaaactta tcaagaatgg gaaatgttaa ttattgatga
    16021 ttgctcaaca gataatagtc ctaatatagt caaatcttat atgcaacagg atagtagaat
    16081 aaaatgtata aagactgaga ctaataaggg tgtctctaat gctagaaatt tagcactaag
    16141 taaggcaaca ggacaattta tagctttttt agatagtgat gaccaatgga atagcagtaa
    16201 gttagaaaaa caagtaaatt ttatgttaga aaatgactat gtaatttcat ttacttcata
    16261 tgaactgatg gatgaaaatg ataaaaaatt aaacaaagta ataaaagttc cacctaatgt
    16321 agattataaa agattattaa aaggaaatat cttaggatgt cttactgttg tgattgataa
    16381 gtctaaattg gattttgaaa taagaatgag tggtgtaaga catgaagact atgttttatg
    16441 gttatcgata ttgaaaaaag ggcatattgc tcatggaata aatgaagttc tagcattata
    16501 tagaaaatct agcaattccc taagtggtaa taaaataaaa gctgctatgt ggacttggaa
    16561 tatatataga aatatagaaa aaataccttt atacaaagct atatactact ttattaatta
    16621 tggaataaac ggaatcaaaa aaagttagac agagaggaag atttaattaa tgtataaata
    16681 taaatttact gtatttactc cgacgtataa cagagctcat ttacttgaaa atttgtacaa
    16741 tgatttaaaa gctcaaactt atgatttcaa tgattttgaa tggttgattg ttgatgatgg
    16801 aagtagcgac agtactaaag agttagttga aaaatttata tcagaaaata aattaaatat
    16861 aagatatata tacaaagaaa atggaggaaa acatactgca ataaatctag gtgtaaaaaa
    16921 tgcagatgga gagctatttt tcattgtaga tagtgatgat ggtttaatta aagattctct
    16981 tgaaattgct aatgatgagt gggattcttt agagaataaa gaaggattta gtggagttgt
    17041 tggactttgt gtatatccaa gtggagattt gatagggaca gaaatgcctg aagataagaa
    17101 aatatgtcac tttgcagacc tatattttaa gtatggcgtt aagggagata aaagcatagt
    17161 atttgtaact gatgagctta taaaatttcc ttttccagaa agaaaagaag tgagattttt
    17221 accagaaagt gtagtatgga atgaaatgtc taaatactat aaagttatat gtgtaaataa
    17281 acctatgata ataagagatt atttggatga tggtttgact aagaatatat taagtaaaaa
    17341 tgcattaaga ggtagagcat tagagttttt atatttaata aatcaaaata catatccatt
    17401 aagtagatat ccatatatgt ggataaaaaa ttacataaat ttagccagat actcattgtt
    17461 gtctgatagt cattattttg gtgaattaag aaaagtatct gataaattgt tgtatttagc
    17521 attattccca ttaggatatt acaaatatat tggacaaaga aaattagtat ctaagtaaac
    17581 ttgttggtat agtactgcta gttgctatat aaggagagta aaaatatgaa tgagccttta
    17641 gtatcgataa ttactcctgt ttataattca gaagagtttt tatcagagac gataaagtca
    17701 atccaaaatc aaacttataa aaattggcag cttttattag tggatgattg ttctaaggac
    17761 aattcaagtg aaattataaa aagttttcga aaagaagatg ctagaataaa atatataaag
    17821 cttgaaaaaa attctggagc tgcagtatct agaaatgttg gtattaagaa tgcggaaggt
    17881 cgatttattg catttgtaga tagtgatgat ttatgggata gtagaaaatt agagattcag
    17941 atagaatata tgttaaaaga aaatgtaggt ttttctttta catcttatag atatatgaga
    18001 caagatgggt caaaaacgaa taaggttgca agagctccta agaaaattga ctatgaagga
    18061 ttacttagaa atactataat aggatgttct acagtggtca ttgataagga gattgtagga
    18121 gagttcagca tgcctttggt aagaagagga caagatactg cgacctggtt acagttactt
    18181 aaaaaggaaa aatatgcata tgggattcag gaggatttag tgaattacag attagttgga
    18241 aattctatat caagtaacaa gataaaagca ttaaaacgaa cttggaatac ttatagaaat
    18301 gttgaaaatt taagtttacc aaaaagtcta tatgtatttt gtttctatgt ttttaatgcc
    18361 attaaaaaaa gagtataagt tttatatact tggaggtaaa aaaatgaaaa tagctatagc
    18421 tgggacggga tatgtaggtt tggttacagg tgtttgtcta gcagaggtag gcaatgaaaa
    18481 tgtagtctgt gttgatgtag atgaacaaaa agttaaatca atgaaacttg gaatagcacc
    18541 tatatatgaa gaaggcctag aagaacttat ggttaaaaat tacaacagag gaagaataga
    18601 ttacactaca gactataaaa aagcttatga agatgtagat gtaatattaa tagctgtagg
    18661 tacacctgag agaagtgatg gctcagccaa cctagattat ataaaggcag ttgcaaagca
    18721 aatagctgaa actgtgacaa aagatactct agtagtaata aaatctacag ttccaattgg
    18781 tacaaatgaa gagatagaag attatataaa tagaaaccta aaaaataatg taaaagtaga
    18841 gcttgcatct aatccagagt ttttatcaca aggaacagca gtaaaagata ctctatatgc
    18901 ttcaagaatc gttataggtg tagaaagtga atgggctaga gaaatattag aagaaatcta
    18961 taagcctttt aatcaaccta taatagttac aaatagaaat agtgctgaga tgataaaata
    19021 cgcatcaaat aacttcttag ctcttaaaat atcatttatg aatgatattg caaatctttg
    19081 tgaaatagca ggagctaaca tagaagatgt agctttagga atgagttatg atgatagaat
    19141 aggaagtaag tttttaaagg cgggaatagg gtatggcggt tcttgctttc caaaagacac
    19201 aaaagctctt cactggttat ctgaacataa tggttatgaa ttaaaaacag taaaagctgc
    19261 aatagaagtt aatgaaagtc aaaaacttaa attagttaga aaggcatcta agttagtaga
    19321 gagctttcaa gatatgaaag ttgctgtttt aggtttgact tttaaaccag acactgatga
    19381 tataagagaa gcaccatcta taccaaatat aaaatatttg cttgaaagag gggcaagagt
    19441 tactgtatac gacccaattg gtgtaaataa tactaagaaa atatttggtg actctatact
    19501 ttatgctgat tctgtagatg atgcgataga agatgcagag atatgtttta taatgacaga
    19561 atggaaagat gtattaaatt atgatataca taagtataag gaaaagatga aaaatccttt
    19621 agtccttgat ggaagaaatt gttataaaat taaagatatg gaagagttag gaattgagta
    19681 ttattcgata ggaaggtaaa tattatgaag actgtttgtt atttagctga tgggtcaaat
    19741 cctcatactt taaaatggtg tagttttttt aaaaataaag gatatgacat tcatgtaatc
    19801 tctttaaatg gaggtcacat ggaaggaata aaaatttata attttggttc taatgtagaa
    19861 gaattaaaaa atgaaaatat ttctaaaaaa acgggatact taggttcaat aaagcagata
    19921 aagaagctag taaatgaaat aaagccagat atattgcatg cacagtatgc aagtagttat
    19981 ggatttatag gaagtctact tgggtatcat ccatatatta tttctgtttg gggtactgat
    20041 atttatgatt tccctaataa tggttttata caaaaacaaa ttataaaaca taactttaga
    20101 aaagcagact atatcttttc aaatagtaga gatatggcta aagaggctaa taaatataca
    20161 gctaaacatg tagatgtaac cttttttgga gtagatatgg ataggtttaa gcctatggaa
    20221 gtagaaaaag aagacgcttt tgtaatagga attataaaga gtttagaaaa aaaatatgga
    20281 atagaatatt taatacaagc atttaagatg cttaaagatg aatataaaga taagaaaata
    20341 attcttaaaa taggtggctc tggaagtcag atggacaacc taataaattt agctaaagaa
    20401 ttgggtatag aaaatgatgt tcagttttta ggaagaatat ctccagaaaa tgtaagcaag
    20461 acttttaatt cttttgatgt aactgtattt ccttctttaa gagagggatt tggagtggca
    20521 gctatagaat cagaagcatg tgaagtacct gttatagtta caaatgtagg aggacatcca
    20581 gaatctgtat gggaaaatga aactggctta attgtagaac caaaacaacc agaagaaata
    20641 aaaaatgcta taataaaact aatggaaaat gatgaactta gacttaatat gggtaagaaa
    20701 gggcgtcaat ttgttagaga aaattatgag gttaatttaa actttaatga tatagaaaaa
    20761 atttatgatt ctatatttga taaatataaa aaataaaatg aaaaaataca gcaaaagata
    20821 gatgttgttg gctatgctta ggtgcgtagc ttttgctgtt taaaggaggg cttctagtta
    20881 tgcgagaaag tatagattta gagaaagaaa gcttttttaa taggcttttt aaaaaatata
    20941 aatttagaca tttaacacta ttaattctag atatttgtag cgtactagta gcgtttcgaa
    21001 tgtctttaag cttgacaggt aatttaaatg catttaccac taatgatata ataatttcag
    21061 tgtgtatata tatagtactt catatagtat catttagatt gtttaagtgc tacaacacat
    21121 tatggagata tgcaggagaa gaagaaatta tatctatttt tgtggcaact ttagcttatt
    21181 taattccgat ttatataata aataaattat tagggtttga ttatcctata atgttttatg
    21241 tattaaatac aatatttatt ataatgttta caagtggcgc aagaatagct tatagagcta
    21301 taagaatagt catgaacaag acatattcta gaggaaaggt ttcaaatatc cttataattg
    21361 gtgcaggaga tgcaggagaa atggttatac aggaactaaa aagaaattca gaacttaaaa
    21421 aagtagcagt ggctataatt gatgatgata aagataaaat tggaagaata atccataatg
    21481 tgaaaattgt aggaacaact tcggatataa aagctgtggt agaaaagtac aatgtagatg
    21541 agataatctt ttcaatagca aacatagaga aacgtagaaa aaaagagatt attgatatat
    21601 gtaaaaacac taactgtaaa ataaagacaa taccaggaat ttacgaaatt atagatggaa
    21661 aagttgatat aaaacagatt agagaagtag agatagaaga cttgttgggt agagagccta
    21721 taaaaacaaa tttaagagag ataagtaatt acattgaggg aaaagtaatc ctaataactg
    21781 gaggaggagg ctcaattggt tcagagcttt gcagacagat tgcaggattt aatcctaaag
    21841 agcttattat agttgataat tatgaaaata atgcttactc aatacaacaa gaattactta
    21901 gaaaatataa aaataaatta gatttaaaga cagtaatagc atcaataaga gaagaaaaaa
    21961 gaatggatga aatatttaat aaatataaac cagaagtggt atttcatgca gcagcacata
    22021 aacatgttcc tcttatggaa tcgagccctg gcgaagctat aaagaataat atatttggaa
    22081 ctttaaacat agctggcttg tcttcaaaat atagagctaa gaaatttgtc cttatatcta
    22141 cagataaggc tgtaaaccct actaacataa tgggagctac taaaagagct gctgaaatga
    22201 ttatacagac tatgaatgct gaaagccaga ctgaatttgt tgctgttaga tttggaaatg
    22261 tgcttggtag taatggtagt gtaattccac tttttaagaa gcaaattgaa gatggcggac
    22321 cagtgacagt tactcatcca gatatcatta gatattttat gacaatacca gaagcagtag
    22381 gcttagttat acaagctggg gctatggcta aaggtggaga aatatttgtg cttgatatgg
    22441 gagaacctgt aagaatattg gatttagcaa aaaacttaat taaattcagt ggatttgagc
    22501 cagatgttga tataaaaatt gaattttctg gtcttagacc aggagaaaag ttgtatgaag
    22561 aactacttat gtcagaagaa ggtctgttgg atacagagca taaaaagatt tttattggca
    22621 gaccaataga tgttgataga gaaaaaataa caaaatactt aaaactttta agagaaatta
    22681 ctaacaatga agaggttgaa aaaatagatg gtattatgag ggaattagtg ccaacttata
    22741 ttaaaccaga agatgctaat atcaaagaaa ttgcaacaac tagagaaaag tagaatatat
    22801 ttctaatttg taaccagggg gagcaaaaag aaacaaatat atactaaaaa gaaacaaaag
    22861 attacaaaaa gattacaaaa agattacaaa aaaggttgca atttattttg atgtaagata
    22921 gaatgttaat taggaattgg aaacgaagga acaaaatgtt aaattttcga aaggattagg
    22981 tatttgagat tatgatgaat attaaaaata aaaaacatat attgaaaaaa tttatagcta
    23041 tggtgttaat agcaggagtt gtcacagtag aagcaggagc aataacggct agtgcagcag
    23101 agccaactaa ttcacctatg agtgcaacag tagaccagtg tgatttttta aatgtaagaa
    23161 gtggagctag tgcaaatgat gcagtagtag gaaagataaa cacaggagac aaagttgaag
    23221 ttttagaatt acattcaaat ggatggatta aaataaaaag tgttgacaat gttacaggat
    23281 gggtaaatgg agattattta actattcaag gtggaaatgt agatgcaaag gtacaaaatg
    23341 ttttaaactt agcatttaag caacaaggta agccatatag atggggagca actggaccaa
    23401 atgcttttga ttgttctggg ttcacttctt atgtctataa aaatggagca ggggttaatt
    23461 taccaagagt atcaagatca caagcaacag taggaaagaa agtatcaaga gcagaattaa
    23521 aaccaggaga tttagttttc tttggttcag gtggttcaat aaatcatgtt ggattgtatg
    23581 taggagattc taaattcata cattcaccac aaacaggaga tgtagtaaaa gttactagca
    23641 tggctccagg aactaattat gcaagaagac ttataactgc tacacgtgtt ttacaataat
    23701 aatagatatt gatttacata ggattgaata ttaacattaa atatataatt acatttaatg
    23761 ttttacatag agatttaaga gtttagtaat attaaaatag tgatgaacgt tataaaaaca
    23821 gcaaagaaga taaatttctt tgctgttttt tttataataa attatttcat attataatca
    23881 aatatcaaat ataaatttta actatattat tcaatatttg acagataata tgaattttag
    23941 atatacttaa taagaagtat attgtagaaa aaataaattt aaaaaattaa gggggatgaa
    24001 atgaaaaaaa taagtatatt agtattatca ttgataatga cacttactat gtgtagtgta
    24061 agttcatttg ccgattcatc aaatgataag gaaatgagag ctgcttggat atctacagtt
    24121 tataatttag actggcctaa gactaagaat aatgaagcca agcaaaaaaa ggaatacaca
    24181 gatttattgg acaaactaaa aagtgtagga ataaatacag cagtggttca agtaagacca
    24241 aaatcagatg ctttatataa atcaaatata aacccatggt ctgaatatct taccggtaca
    24301 caaggtaaag accctggata tgacccatta ccatttttaa tagaagaagc acataaaaga
    24361 ggaatggaat ttcatgcatg gtttaatcca tatagaataa ctatggcaga tgagagcata
    24421 gataaattgc cagcaaacca tccagcaaag aaaaatccaa gctgggtagt taaacatgga
    24481 aataagtatt actacgaccc aggtttacca gaagttagaa agtacatagt ggatagtata
    24541 gcagaagtag ttcaaaatta cgatatagat ggagttcatt ttgatgatta cttctatcca
    24601 ggagtaagtt tcaatgatac tgcaacatac caaaaatatg gaaaaggaca aaataaggat
    24661 aattggagaa gagaaaatgt aaatactcta cttagagatg taaaagcgtc tataaagagt
    24721 ataaagccta atgttgtatt tggtgtaagc ccagcaggta tatggagaaa taagagtagt
    24781 gacccaacag gttcagatac atcaggtaat gaaagttatg taggtacata tgcagataca
    24841 agagcatgga taaaacaagg attgattgac tatgtagttc cacaacttta ctggccaatt
    24901 ggtcttaaag cagcagatta ttctaaatta gtggcatggt gggctaatga agtaaaagga
    24961 acaaatgtag acttatacat aggacaaggt atatataagc aaggtcaatc tagttatggt
    25021 ggacaaaata tagcaaaaga aatagtacaa caagtaacat taaatagaaa gtatagtgaa
    25081 ataaaaggta gtatgtattt ctcagctaaa gatatagcaa atagtacttc tatacaaaag
    25141 gatttaaaga gtttatattc tagtagtgaa gaaccagtta ctccaccatc taatgttaaa
    25201 gtggagaaat taagaggcga cgaaagatat gacacagcag tagcaataag taaaaaaggt
    25261 tgggctacaa attcagatac agtagtatta gtaaatgggt actctatagt tgatggcata
    25321 acatcaacac cacttgctac atcaaatgat gctcctatac tattggtaaa taaggataat
    25381 atacctacat ccactaagaa tgagttaaaa agacttaatc caagtaaagt tattttaatt
    25441 ggaggaaata actctattgg agataaagtt gagagtgaga taaaagatac attgtcaaat
    25501 gtatcaataa atagagttgg tggttcagat agatatagta catctttaat gatagcaaaa
    25561 gaattagtaa aaactaatcc agtagaaaaa ctatatataa caagtggtac aggtgaagca
    25621 gattctcttt ctatagcttc aaaagcagga gaagaaaagc aaccaatagt actagtatca
    25681 aaagataatg taagtgatga agtttacaat tggatatcag atttaaaagt aaaagatgct
    25741 tattttatag gtggtaatct aagtatatct gattcagtaa taaataagct ggataaggtc
    25801 attacaaatg atgtatctaa aaatagaata gctggagaaa atagagaaga aacaaatgga
    25861 aaagtaatac aaaaattcta tccaaatgct gaatattcat caatgtttgt aagtaaaagt
    25921 aatcaattgg tagatgcatt aacatcaggt cctttagcag ctaaattaaa atcaccagta
    25981 gttatgcttg gaaatagtgt aacatctgct cagaaaactg ctttagagcc taagaaaact
    26041 gctctagtat acgaagctgg agatggtata aatcaaaaca cattaaacac tttcttaaac
    26101 ttagttaagt aataaaaaag ttaatttaaa atacagagta ttttataaat gtagattaaa
    26161 atgaaaatta tcatcataaa ttagaaaata aaaaagaact atatgcattt tgtaatcaag
    26221 tgtacacagc aacttgaaaa taattatgcg atatagttct tttttagtaa ttaatcttat
    26281 actttttaat aaactttatg ttgaattttt tattgtttaa actctatgtc attaaatata
    26341 aaatcgtgta atatgtctaa agtatctggg tcaaatctaa gaacccatcc agcttttcca
    26401 tatataccac ctgtactatg aatttcatca tcaataggaa attcaaattg cttaatattt
    26461 aagtcaccca tttttaaaac ttgagctcct aaagaaagta ttgcattagg cttcatgtta
    26521 gttttaacat atggaagaac tgcatctaat aaacctggat atttagtaac aggtaaagtt
    26581 tccgttttct tgattatagc ttgtataatc tgtctctgtc tgccatctct agcaaaggct
    26641 gtatcgttgt gtctgatacg tgcaaaagat agtgcctgat atccatttag agtctgcttt
    26701 ccagagtttc taatgtattg tatgctacct ttatcatcac ttttattcca tttataagtt
    26761 tcaggaataa atttatttag ttctcgtatt tctccttttt caacagtaac ctcaacgcca
    26821 ccaatagcat caattatata cataaatgat tcgaaattaa cacaagcata attttgaata
    26881 tcaatattaa aattctcttc aattgtttgt attaataaat cagcttgtcc ataagcataa
    26941 gcatgagtaa gtttttgttt accatgacca gggatgtcta catagctatc acgagctaat
    27001 gatgtaagtt tgatatcatt gtgtttgtta tcaatagtaa gtatcatcat agcatctgac
    27061 cttgaagaat cttcatttgg tctagcatct gttcccataa gtagtatgtt aattatccca
    27121 tcttcatttt tatggtcatt gttatccaat aaatcactac ttatgctcga gtcatgaata
    27181 gcagataatt tataataaaa atatccatag acacttatag ggaatattac taccaagaaa
    27241 gccagtagta taacaaattt ctttaatttt gacaaaagta ccccttcttt cttctttttc
    27301 ctgtatattt tttaatagca tacacttaat tatattaaag tattgagata tattcaatac
    27361 ttgtttcagt tacaaaaaaa agacaatgat ataatttata agaaaattta tcgacctata
    27421 tgtatagtta aaagtatata taatatattt tttaaaagta atttatattt taaaataaaa
    27481 acacacccca taaatacaaa gcaatttgca tttaagaggt atgtatttat catcaaatta
    27541 ttggtttgtt ttggaatttc taatcttcaa ccataatatc tttaaatatg aaatcatgca
    27601 ttgcatctag ttcatattcc tcaaaaggta ttacatagcc tgcattacct atttttctac
    27661 cattttcagt agaaagagga aactccatag atttcaaatt taaatttcct atacttaaaa
    27721 gttcttttcc taaagatatt atttcattag gattcatatt agtttttaca tacggaagta
    27781 ttgtgttaac tagattagga tatttagtga ctggtaaatc cttaacacca tttattaaac
    27841 tctgtataac acttctttgt cttctatctc tttcatctgt actatcattt tttcttatac
    27901 gagaaaatgc aagtgtctga tatccattta atttttgctc accagaattt cttatatatt
    27961 taagagaacc tttattgtca tttttattcc atttataagt ttcaggaata aatttattta
    28021 attctcttat ttcactctta tcaacagtaa cagtaactcc acctaaagca tcaactatat
    28081 ccataaaaga gaaaaagtca actacggcgt agttttgtat atcaagttgg aagttttctt
    28141 ctatagtttc tattaataag ttagcttgtc catatacgta ggcatgtgtt aatttttgtt
    28201 ctccacgtcc tggtatatca acataagtat ctctatttaa tgaagttagc ttaagacttt
    28261 tatttttatt atcaatagta agtatcatca tggcatcaga acgagaaact tcttctcctg
    28321 gtcttgcatc tgtacctaac aataatatgt ttgtgatatt ttcttcgttt ttatagtcat
    28381 tactactaag cgcctctgta tttatactat catcttgtat agtttttaat ttagtaaaaa
    28441 catatccata tatactaaca ggaaatgcta taactaaaag ggctagaaat ataacgaact
    28501 ttttgagtcc tgacaataat atcccctact ttctaaattt tttaattaca catagttaat
    28561 tatattaaaa atttgttaca taagcaataa aaaacacaat aaattagcat aagtttgtca
    28621 taatataatt atttttatag tattttatag aagaaagtgt gtagttatta ttgattttat
    28681 tggaatataa ttgtatgaag acttaaattt ttataaactg atgtgtatat tgaaaaaaat
    28741 attttcctga atgtagttat agcatcttat gaaaggtgta aattgtaatt taaaattttt
    28801 agtgtataat aagaattgaa aattcaaacc gataaaaatt aataaaaaat aattataaat
    28861 taataatttg ggggaggaaa tgtatgataa aattaatagc aacagattta gatggaactt
    28921 tgttagatga aaagagcgaa ataaatccag agttttataa agtatttaaa aaattaagag
    28981 agagagggat aatgttttct gctgctagtg gcaggcagta tcaaaatctt ataaaaaaat
    29041 ttgaagatat aaaagatgat atgatgttta tatcagaaaa tggaacacta gtagtatata
    29101 aaggaaaaga aatattatca aatcctttaa ataaagaatt agtaaatgaa atcatagaga
    29161 caacaagaag tataaaaggg aaaaaaattg taatgagtgg gaaaaaatac gcttatatag
    29221 aaagcaaaga tgaagcgttt atacaagagg ttagtactta ttatgcaaag tttaaaattg
    29281 tagacgattt gacgaaagta gaaggcgata tattgaagat agctgtattt gacttcaaag
    29341 gtgcagaaca taataataat atctactttg aaaagttttc tgatagagct caagtatgta
    29401 tttctggagt ggagtggctt gatttaactg caaaaggtgc aaataaaggg agtgccataa
    29461 aaaaagtaca aaaaatgtta gatataaaat atgaagaaac tatggtattt ggtgaccagt
    29521 taaatgatgt ggaaatgatg aaaagtgcct atcatagtta tgccatggaa aatgcaaatg
    29581 aacatttgaa acaaattgct agatttagag caaaaagaaa tactgaaaat ggcgttgtag
    29641 ataagataaa agaggtcata aaaattggat agtcatatgt atagatgaaa aagattattt
    29701 tgaagaaatc aaaaaaatat caatgtaatt ttgataaata agtttattta tgcgaaaatt
    29761 acattgatat ttaataaaca tcaataatag atatttattt ttttatgata ttcagtattt
    29821 tgttttctat ttgagttgca ttttcagcag aaattaaata aacatatcta cctgctttct
    29881 caattatagt attatttgct atttcagaat tgattttttc tccatgtcct tctccaaaag
    29941 gtgcttgtat catatctttt ttataatttt ctagagtttt atatatttta tctacctcgt
    30001 cagtttttat aacgataata tcttctagag ataatttttc agaagaatta attacaaaac
    30061 cttgaattat gtcttcctct acatcattaa aatagttaaa gtcctttgca tcaaagattt
    30121 ttgattcttt tataagtaaa tcagaagatt ttaatgcttt ttttattttt gatgtagaaa
    30181 cgtttttgta atcaccactt gaagaacaac taaccattcc taatgataag attatcgaaa
    30241 aaaacaatat taaaattttt ttcatgataa acctccaagt tttttaaact ctgtcttaaa
    30301 ttagtttact acataattat ataaaaataa atgtatttaa tgttacagtt ttgattaata
    30361 atgtaatatt ttgataataa tacctattga gaggtgatta gaaatgagaa taccaaaaca
    30421 tgtaggaatt ataccggatg gaaatagaag gtgggcaagt caaaatggta tgacaaaaga
    30481 taaaggatat gaaagtggaa tagaccctgg attagagtct tatagattat gtagagattt
    30541 aggctgtgaa gaggtgactt tttatggatt tacaactgat aatacaagac gtccatcagt
    30601 gcagacaaaa gcttttacaa aagcatgtat tgatgctgca aatatattaa tagatgaagg
    30661 tgctgcttta ttagttgtag gaaatacaaa atctccaatg tttccagaag aattattacc
    30721 atatactaat agaattaaag ccaaagaagg tcaaataaaa gcaaatcttc tggtaaatta
    30781 tgggtggtat tgggacttaa ataatatatc aggagtagaa aatgtaaata aaaataatat
    30841 acaggattac atacagtcat ttgatgtatc cagactagat ttaataatca gatggggtgg
    30901 aagaaggaga ttaagtggtt tccttcctgt acaatcaatt tatgcagatt tttatgttat
    30961 agataattat tggccagatt ttaagagtga cgatataaag gacgcattta gttggtatga
    31021 tacacaagat gtcactttag gtggataatt aaaaatattc tgatttaagg ttgtagagtg
    31081 ttttaaatta ttgaaaaaaa aacttcttga tggtaaaata acactaataa tatgtaaaag
    31141 tgtaaaaatt tataccaaga agggtgaaga aatgtcaaaa ggcaataata acaataattc
    31201 aagaaacaaa tctaaaaaga ccagtcatct gaatagaaaa aagcgaaaat taaataagaa
    31261 aaagttagca gtgttgattt gctttacagt tttattttta tttattgcat ttaaggctac
    31321 tcaaggtgta gtagctttgg taaaaagtat ggataaaagt aataaaacta gtcaacaaca
    31381 aaaagttaac tcagaacaat ttgaccttgg taatgaagag gaaaataaaa agaaaaaata
    31441 tactgtattt atagacccag gtcatggagg caatgataaa ggaactgaaa gcaagaccag
    31501 taatagatat gaaaaagacc taaatctaca aattgcaaaa aaattagcaa ataaattatc
    31561 taaacaaaag gatattcaag tagtagtatc cagaactgat gatacatata tatcattaaa
    31621 ggatagagct atcttagcaa ataatagttc tgctgatgtg cttgtttcta ttcatttaaa
    31681 tgcagagaaa aatggtaata cagcaacagg tatagaaact tggtatagga ataaagctac
    31741 agatggttca aaagaacttg cacagacagt acaaagtact atagtatcct atgttaaagt
    31801 tagagatagg ggaatagttg aaaataactt tgaagtactt agagaatcaa atatgcctgc
    31861 tatattaata gagtgtggtt ttttaacaac accttcagag gaacaaaaaa taataaatga
    31921 aaaatatcaa gaccaacttg cggaaggaat tgttcaaggt gtactttcat acctagattc
    31981 taaagggaat aagtaagtta actttacttt aatgtgagga taaatttaaa agtttaactg
    32041 tgtaaattgt attaacttta tttcgtaatg ttggaaacat tgtttgaatt tagaaactat
    32101 gaaattagaa attattttat ctatatctaa tttcatagtt ttttttgcaa aaaatttata
    32161 gttataaaat ttaaaaaaat aaatttagta taatgtaatg tagttattta tatgtgattt
    32221 taacttgcat tgatttgcta tcaattgtgc tttataatta gtatatataa agtttaactg
    32281 taagataatt taaaa
//
LOCUS       DACTGW010000018        31434 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.018, whole genome shotgun sequence.
ACCESSION   DACTGW010000018 DACTGW010000000
VERSION     DACTGW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.018"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            201..482
                     /locus_tag="I9573_000677"
     CDS             201..482
                     /locus_tag="I9573_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795311.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            746..1000
                     /locus_tag="I9573_000678"
     CDS             746..1000
                     /locus_tag="I9573_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795312.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            1045..1368
                     /locus_tag="I9573_000679"
     CDS             1045..1368
                     /locus_tag="I9573_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795313.1"
                     /translation="MATEIFTVKITPLEAQKMIKENIDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTEKTVIKATSTGSAIGVLKIDWGAGGNLLKRVKNIL
                     AEYII"
     gene            complement(1928..2122)
                     /locus_tag="I9573_000680"
     CDS             complement(1928..2122)
                     /locus_tag="I9573_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT4795314.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYEM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(2496..4115)
                     /locus_tag="I9573_000681"
     CDS             complement(2496..4115)
                     /locus_tag="I9573_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAT4795315.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGNCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            complement(4424..4630)
                     /locus_tag="I9573_000682"
     CDS             complement(4424..4630)
                     /locus_tag="I9573_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4795316.1"
                     /translation="MFKKKSGYTFTNFLNKVRVEKNKKYLLKKDLSILDVTVLIGFNS
                     QNYYSIIFKKFNNLTPIEYKNIYN"
     gene            4935..5126
                     /locus_tag="I9573_000683"
     CDS             4935..5126
                     /locus_tag="I9573_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795317.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(5215..6027)
                     /locus_tag="I9573_000684"
     CDS             complement(5215..6027)
                     /locus_tag="I9573_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HAT4795318.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            6449..7600
                     /locus_tag="I9573_000685"
     CDS             6449..7600
                     /locus_tag="I9573_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4795319.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDDDLDKMLELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TESLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            7718..8356
                     /locus_tag="I9573_000686"
     CDS             7718..8356
                     /locus_tag="I9573_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HAT4795320.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            8357..9379
                     /locus_tag="I9573_000687"
     CDS             8357..9379
                     /locus_tag="I9573_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HAT4795321.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEYIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRINYKELDR
                     IDIDEL"
     gene            9369..9989
                     /locus_tag="I9573_000688"
     CDS             9369..9989
                     /locus_tag="I9573_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795322.1"
                     /translation="MNYNSNKNKYLLKDINLEDIFIDKNIVLKFLGYGNRKAPDTISE
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGIALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(10113..10799)
                     /locus_tag="I9573_000689"
     CDS             complement(10113..10799)
                     /locus_tag="I9573_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAT4795323.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGNSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(10813..11358)
                     /locus_tag="I9573_000690"
     CDS             complement(10813..11358)
                     /locus_tag="I9573_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4795324.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCIDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            11586..12833
                     /locus_tag="I9573_000691"
     CDS             11586..12833
                     /locus_tag="I9573_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HAT4795325.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            12920..14914
                     /locus_tag="I9573_000692"
     CDS             12920..14914
                     /locus_tag="I9573_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAT4795326.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIKNTFSENTN
                     KTSEIEIYNKILKINTFPVEYKKNNTEYIVVLIKDITNEKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            14914..16236
                     /locus_tag="I9573_000693"
     CDS             14914..16236
                     /locus_tag="I9573_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAT4795327.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            16515..17801
                     /locus_tag="I9573_000694"
     CDS             16515..17801
                     /locus_tag="I9573_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAT4795328.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            17887..19194
                     /gene="grdB"
                     /locus_tag="I9573_000695"
     CDS             17887..19194
                     /gene="grdB"
                     /locus_tag="I9573_000695"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:18934..18936,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HAT4795329.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVVTGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            complement(19410..19634)
                     /locus_tag="I9573_000696"
     CDS             complement(19410..19634)
                     /locus_tag="I9573_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAT4795330.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(19731..20984)
                     /locus_tag="I9573_000697"
     CDS             complement(19731..20984)
                     /locus_tag="I9573_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HAT4795331.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIGLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            21244..22233
                     /locus_tag="I9573_000698"
     CDS             21244..22233
                     /locus_tag="I9573_000698"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAT4795332.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            22305..23045
                     /locus_tag="I9573_000699"
     CDS             22305..23045
                     /locus_tag="I9573_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HAT4795333.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            23245..24396
                     /locus_tag="I9573_000700"
     CDS             23245..24396
                     /locus_tag="I9573_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HAT4795334.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLEPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            24421..25176
                     /locus_tag="I9573_000701"
     CDS             24421..25176
                     /locus_tag="I9573_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HAT4795335.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKNTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            25368..27755
                     /locus_tag="I9573_000702"
     CDS             25368..27755
                     /locus_tag="I9573_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HAT4795336.1"
                     /translation="MKKFTSKKVARFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SDTYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSVKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVSYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFNIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(27986..28219)
                     /locus_tag="I9573_000703"
     CDS             complement(27986..28219)
                     /locus_tag="I9573_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAT4795337.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            28508..29098
                     /locus_tag="I9573_000704"
     CDS             28508..29098
                     /locus_tag="I9573_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT4795338.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRVVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            29212..30057
                     /locus_tag="I9573_000705"
     CDS             29212..30057
                     /locus_tag="I9573_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4795339.1"
                     /translation="MIKVDDLSFSYTDRNFLQNINFEVEKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIKKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            30054..30731
                     /locus_tag="I9573_000706"
     CDS             30054..30731
                     /locus_tag="I9573_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4795340.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILISIIIIIYFITYYFICSSWK
                     KVGGVKL"
     gene            30728..>31434
                     /locus_tag="I9573_000707"
     CDS             30728..>31434
                     /locus_tag="I9573_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4795341.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGTLAFIITMILLIFFSLTKISFLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKK"
BASE COUNT        12452 a         3370 c         5067 g        10545 t
ORIGIN      
        1 acaataattg taaagtaaaa tctcaaaata tacaattatt ataaatcata gatatttatt
       61 aaataaatat attaaagaag aaggaatata aaacaagatt agaaaataat agtgttactt
      121 tcataataaa tacatataaa gatattttta agaataaaat ttattattaa ataaaaaata
      181 aaggagagtg atttaatcgt atggcatcaa aacaaacacc aagaaacaat ttagaaaagt
      241 ttttaagaat aagagtctta tcacatacag gcgataaaat acatataaag ttacctgtaa
      301 attttgcaaa aagaatgatt gagaataatg ctttggattt atttaatgga aaagatgatg
      361 tagtagatgg taaaaaggtt actgaaatat tagtcaaagc atttgactat gatttaactg
      421 gagaagtagt tcacttggag agaaaaaatg gagatattat taaagtttca attgtacaat
      481 agaattattt tatatgaatt ttatttttga atagttataa ataattagaa atttgtaaaa
      541 aagtataata tattattagt aatggtgcag aatacacatt tataatttat agatgtgtat
      601 tttttgttta aaaaatttaa atatataaaa ctataaaggt tacaattttg gaaatagatg
      661 taaatatatg ttctattgta taataagagt aatataaaat ctagtaaaaa taatcaaata
      721 aatttaactt atgagaggga taactatgga aaataaggat tttaaattaa caatatgtat
      781 atttgctttt gtaattggag tagtagtatt attctgctgt ataggtttgg gtgaatttag
      841 tgcagagcat attattcaac aaaatggtgg aatcatggat actgaccaat ataatattta
      901 tctagaccaa tctatttctc aatatagaaa ttttggttct atattagctc tgttaggagg
      961 aatagggata ttgtttgata aatatatcag aaaatactag agattatgtt ttgtctaaaa
     1021 taaattctta tgaggagata aaaaatggca acagaaatat ttactgtaaa aattacaccc
     1081 ttagaagcac aaaaaatgat aaaagaaaat attgatgcag atttagtttt tagtgattct
     1141 tataatttag gagatgataa atttatacta attacaactt ttgagaagta ttacgcaaga
     1201 aataatagtg atgcaggatt aatcgtagtt tatgaaaata ccacagaaaa aacagttatt
     1261 aaagctactt caacaggcag tgcaattgga gttttaaaaa ttgattgggg tgcaggtgga
     1321 aatttactta aaagagtaaa aaacatactt gctgaatata ttatttaatt attttatcta
     1381 actgataata tgtcaagaaa atctcgtaaa agaggttaag aatattttta taatatatag
     1441 ataaataagg atttgaaggt aaagttttta atgtataaag aaaaataata taataaaacg
     1501 aattggcttt ataattcttt ataaggcaaa gagtacattt ctttatataa ttgttggata
     1561 ttaaataata tgtaaataca ccatttacat ataaaagttt aattaatatg ttaaaagaaa
     1621 atgaaatgga aattactaaa aaatactttt aaattggata atgattgata ttacgtttat
     1681 acaaaaatca agtagaattt tatatatttt aaatatgata gtttaaaatt atgaaagaat
     1741 gataaattcc agtttgtagg gtagttttga taaataatat taataaatat gagtaaaaag
     1801 taggagcata ttaatataat attcctactt ttttattatt aaaattgttt atgatatcaa
     1861 cactacatta aaacacaacc taaatttgaa aaacaaatag tttagaaata tgtacttttt
     1921 ttatttatta tattctttta aatcgaattt tttcatctga acataaagtc tcaaatctat
     1981 atgcatctgt catctcataa aaaccaaaaa atataaagcc atcacgagaa gtaaattcat
     2041 attgtatatt ttcaattttc aacatgtttt ctacttcgct tattttttcg ctatatataa
     2101 tttctaccat ataatttatc attgtagaat ccctcctatt aatatgaaaa tatccaaata
     2161 tataataaca tatttagatg atataatagt atccaaataa aaaataatct tattacatta
     2221 ttgaatgaag aattaaaatg aagataaata aaataaatat tggttataat tgattaaaaa
     2281 atagttatgt atacttgtga ctttagttat gggcagttca agttttatga aagaagtcat
     2341 atatatatgg aatattctag gcagaagctg tttaataata agagaatact ataaaaattt
     2401 gagtattaca taaaaaataa gaagccataa atgatataag cattatctat agcttcttac
     2461 ttctcattga ttaagattat atataaaaat aatcactata ttttttgttt aagttttaat
     2521 acttgtttta ctatatttgt tgagtggtca gaaattcttt ctagattagt aagtaagtca
     2581 agataaagta caccagcatc aattgtacaa tttccattat ttagtctaat catatgtgaa
     2641 tttctaaaag ttttttctaa ataatttact tcattttcaa cttctaaaac tttatttgct
     2701 aactcaaaat ctttaacatc taaaactatt aaggcatcct taaaattagt aattgtctta
     2761 ttgtatattt cgtatatttc tcttatcgca ctatcagaaa aactaatgtt catttcctta
     2821 acactcatgg ccaattcagc tatattttct gcatggtcag agactctttc aatgtcattt
     2881 acagtattaa aaagcaaatc tgttctaaag cgttcatcgt ctctaagagg ttctttagaa
     2941 agttttaaca aatagttcaa tatatctttc tgtaacttat tgacaacttt ttctgactca
     3001 aaagctttat taatatcttc attgctctta tttaaaaatc ctgcaattga atgttccaaa
     3061 gagtttgtag ctttatgacc cattcttaca acttcttgta cagtattacc aagtgctata
     3121 gatggagtgc taaacatcct ttcatctaag tacttagtat tatttactat ttctgattca
     3181 gtttctttaa taggtataag tctatttgct aatttaacta tgtatttaga aaatggtaat
     3241 aatattataa catttgtaag attaaataaa gtatgtgcat ttgctatttg tctagctgta
     3301 tcagttgggt caaaatgagt aactatggct gatataggtt tatttaaaac aagcataaat
     3361 attattgtac caatgacatt aaaacttaaa tgcattacag ctgcacgtcg agcatttcta
     3421 cttgcaccaa cacttgataa aagtgaagta acacaagtac ctatattatc tccataaaga
     3481 ataggtaaag cagatgatag aggaagtatt ccttgagaag ctagtgctat taacataccc
     3541 atagatgcac ttgaactttg aacaatacca gttatggcaa atcctgccaa tatacctaat
     3601 acaggatttt tactaaaata taaaagtgca tcagtgaatg ctttatattc agcaagtgga
     3661 gctactgcat ctttcataaa ctccattcct gtaaataata taccaaaacc tataagtatc
     3721 tcagataaat gctttgtttt ttgatttgat gtaaataaat agaataaaat acctatacct
     3781 aaagcaattg gtgcaattcc ttcaagatta aatgaaacta attgagcagt aactgtagta
     3841 cctatatttg cccccattat tacaccaata gcttgactta gattcattat acctgcattt
     3901 acaaatccta caaccataac agtagtagca cttgaacttt gtataatacc tgtaacgact
     3961 gtacctacca gtacacccat aacaacatta cttgttaaga gttcaataat tttttttaat
     4021 ttgtctcctg ctgatttttg taaaccttct cccattaaac tcattccgta aagaaaaagt
     4081 ccaagtccac cgataaggct aattactata ttcacgatat ttcctccctt aagaaattaa
     4141 tagttttttt atctgaatta taaactttat taactaaaat ttattgtttc cataatatag
     4201 aataacaaat tttgtcaata gaaatattaa tgttttgtta agtatacgtt aagagaatgt
     4261 taaattttta agttgttgtt aattttagaa aatttatgat atatagcaat ttaaatactt
     4321 gaacttagat ttttagtatt ttatgtacaa tcaatttaac aaagaattaa ctataattgt
     4381 aaaaaatatg taaaaaaata gcatcctttt agaatgatat tttttagtta taaatatttt
     4441 tatattcaat tggtgtaagg ttattaaact ttttaaaaat tatactatag taattttgac
     4501 tattgaatcc aattagtaca gtaacgtcta aaatagacaa gtcttttttt aatagatact
     4561 ttttgttttt ttcaactcta actttattta aaaaattagt aaaagtatat ccactttttt
     4621 tcttaaacaa agaaaaaaat aacttttatt aagttgtaat tcattacata tattacttac
     4681 tgttatatct tctgagtagt ttttgtgaat ataatctata gttttcttta agttttcttt
     4741 acataaaact aagagttggt ctatgtttca ttttatcaag gcatatttat taaaaaatca
     4801 atctctattt gttaaaataa tttatgaatg atgttagaaa ctgtaatata ttggatataa
     4861 tataataaac agataaactc tgtattaaga ggatatatag tttatcttaa ttatagggga
     4921 agtgaggata tattatggaa agattaatgg aactcataga aagaaaattt ttaaaggatg
     4981 tagaactttt agaattagaa gacatagaaa atgtaataga atgcaaaatg acacagccat
     5041 ataatgctca ttataatgaa tacttagtaa aagtaaaaaa tggagatgaa gtagaagaat
     5101 attttgtgta tttaaaacat agataaacat aaaagaggtt tatatttatt tgtatgatta
     5161 taaaataaat acaagcctct ttttttgata aatataaaat tataaaattt ttatttattt
     5221 aattttttca acaacattag tcaatattga atctaaatca tcactaattt ctgtaactcc
     5281 attatgagta atatctataa tttctttagt cataggaagt tctcctaaaa gctctaagtc
     5341 taaatcatct aaaaactttt cagtagattc tccttcaaat aacttgattt ttttagaaca
     5401 atcaggacat tggatataac tcatattttc aataactcca aggacattta tgttcatttt
     5461 tttagccata ttaacagctt ttgatacaat catagatacc aaatcttgag gtactgaaac
     5521 cataactatt ccacttatag gaatagattg cataaccgtt aaggcaacat ctccagtacc
     5581 aggaggcata tctattaata aatagtctaa ttcttcccaa agtacatcag tataaaattg
     5641 ctttacaact cctccaagta aaggtcctct ccaaacaaca ggttcattct catcatctat
     5701 cataaggttt atagacatta cttttatgtt atttgagttt tctacagggt aaatataaga
     5761 gccatcagaa taagctttta cattttttac acccataagt ctaggaatac ttggtccagt
     5821 tatatctgaa tctaatatac caaccttata tcccattttg tttagcttat tagcaagtaa
     5881 agcagttaca gttgattttc caactccacc cttcccactc ataacaccaa ttatttttcc
     5941 aaatttattg ttagggttat tttcaataga acaatttgat tgactattac aatttccctt
     6001 tgatggacat gaattacaat ctgccatatc tattacctcc aaactttaat atttattaga
     6061 ttatttgtaa cctacatata ctattttagt acaattgaac cataaagtca ttatttaaaa
     6121 gcaacttaga agattttcta ttgatttatt catagactat agcaaaaaga atacaataaa
     6181 aaaattataa cattcttgaa aaagtgatta tttcaattta gttatagaac ttagtcatat
     6241 aaatatatag aaaatatttt aatatattat acataacggg ttattttata agtttaacta
     6301 taaattaggt ttatatttta taaaaatttg ttaaaaaaaa caattaaaaa ttgtaaagaa
     6361 aaaagatata attagaactg tagtaataaa tgttttatta tagataatag agttttaagt
     6421 atcttaaata atatttggag gaatatgaat ggaattaaga ccaagaagtg caaaattaga
     6481 agaatttgat aaagtaatag aattaataaa ctatgtattt agaatatcaa gaaatcataa
     6541 gccaactatg atggaagaat ttcctctttt actaagtaaa aataatatag aaaatatgat
     6601 tataatatca gaagatgata aagtggtttc tgatgtaaac tatctaattc aagatgtatc
     6661 tattcaagga aatagattga aggttgcagc tataggtgga gtatgtactc atccagatta
     6721 tgaaaagaga ggctattcat ctaagatatt ggataaagta gaagaaaaaa tgttttatga
     6781 tggtgtagat attgtaatta tatcaggaac aagaagtcta tacagtagaa gaaattgtag
     6841 tttggtaaag agtttttata aatatacaat aaaacctgaa gatgtaaaaa tagcatatga
     6901 aatagttgaa tttgatgaaa ctaattttga aaaagatgat gatttagata aaatgcttga
     6961 actatataat caaaatagta ctagatttat aagaacgaga gatgagtttc aaaaactatt
     7021 gcatgcagca actattgcct ggggaccaat tggttacaag aaggtattta taaaggaaaa
     7081 taataatata ataggatatt tgataataag aacaataaaa aaagaagatt ctactgtagg
     7141 agaagttgct gaaatagggt taaactctgt aaatgttgaa aatatattga aatatgttgc
     7201 aaataaattt ggattagaat atttaaatta taaagtacat gtgaaaaatc ttaaagacca
     7261 gttgaaatgt aatgggacag aatctttgga ctatcaacaa ggaactatga aaataataaa
     7321 ttttactaag ttgtgtgata gtttaagaag ttattttagt caatatgtag attttgaatt
     7381 actaaaatac atggaattta aacaagttga aaataaatat attattaaat acaaagaaga
     7441 agaacttgta atagaaaatt tggataagct taataaactg ttttttgaaa aaaatgagga
     7501 acagtataat gaatttaaac atctaaaaaa tatatatgaa tttgctacaa aggcatttcc
     7561 tgtagatttt ccatggactg caaatttaaa ttatcaatag ttttagatga tattaaataa
     7621 aattgattga tacctagagt tagtccttgt taaataagag aaattattgg atattgaaaa
     7681 agcaattttg taagtaatta gaaaggatga gtattatatg gacatacttg atgatatagc
     7741 agaatgtata ttaaatatgg gtgtagataa tatagagaat ttagtaaaaa ttgctataga
     7801 taaaaatatt gatgtggaag atatttatga atatggttta aacaaaggta tgataggagc
     7861 tttagataag tttgagaata aggaatacta tctttcagaa gttatagtat gcacagatgc
     7921 attaaataaa ggtattaatc ttttgaaggg aactggaaaa gtaaagaaaa aatcaaaggg
     7981 agtaattctg atgtctgtag tagaaggaga tactcatgag ataggaaaaa atattgtaaa
     8041 agtaatggta gaagccactg gatataaggt tattgactta ggtgtaaata gaaaaagtga
     8101 agatataata gaagaagcta taaaaaacaa tgtagatata ataggcttat cttcaatgat
     8161 gacaactaca atggaaaata tgaaatcggt tatagacgag cttaatatga ttgagataga
     8221 taaaagacca aaagtaataa ttggtggagg acctgtaagt atggagtttg cagaagaaat
     8281 aggagcagat ggatacagtt caaatgctcc aaaagcagtt aaattaataa acaaacttat
     8341 aggaggtgaa gtttaaatgt tttcagatat gactttactt cagtatataa atagtgaaga
     8401 tagaggtttt tttctgcctg atatgggtac aaatggattg ttcttaataa ataaaaaggc
     8461 atatgaagta tatgatagtg tagatttgca attagaatta gcaaagacta tggacaagta
     8521 ttttgaatca gattttatat actcattctg tgatgggatt acattttgtg aaactctggg
     8581 tttagagaca ttaagaccag attatgattt tccaagtgta ttgacacata ctataactga
     8641 cagagataaa ttaagaaaat tagatgttcc agacccttat acgagtggac gtatgccact
     8701 caatatagag agcttatcag ttatatcaaa aagtattaaa aaaccactat atgaatcgat
     8761 acaaggtcca tttacacttg caggtcaatt agctggagct actcaacttt taagatgtat
     8821 aataactgat aaaaaatttg tagaagaatt gttggaattt acaactgaat tggtaagaag
     8881 atatgctgta gctgcaaata aagcaggagc aaaatatatt tcaatagcag agccaacgtc
     8941 agtaacccta agtaaagata gatttgatga atatatagta aaaaatttaa ataaaatcta
     9001 caatgaactt gattgttgga aaggtatgca tatatgtgga gacaccagag aattattaga
     9061 taatatgcta agttgtaata tagatgcagt aagtttagac caaattttag actatgaaga
     9121 aattgctcca atgattccta gagatatagt gttgattggt aatttagacc caataaaact
     9181 tcttggacgt tctaagccag ataagattag aagagagaca ctaaaattgt taaagaagat
     9241 gagaggatat gataactttt tatgtgattt tggctgtaac tgcttaaata ccactccagt
     9301 agaaaattta caagcagcta taaaagcagg aagaattaat tataaagaat tagataggat
     9361 tgatattgat gaattataat agtaataaaa ataaatatct tttaaaggat ataaatttag
     9421 aggatatttt tatagataag aatattgtgc taaagttttt ggggtatgga aatagaaaag
     9481 ctccagatac aattagtgaa atcgttgaaa gagaaataag aaatataaat gatattttag
     9541 atatcaagat atatataagt gaagttgata ttaataaaac acctaaagaa tgtaaaaaag
     9601 catttgctgt actatataca ataggggata aaattgatat taagatgaat gactatatgg
     9661 aaaattgcaa tatgatggca gggatagcat tggataaaat aggtatagta tgtttagatt
     9721 atattaatga gaaaataaaa atgtatttaa aaggaaagta tgatagtctt aaaatatcgc
     9781 atgaaatata tccaggagat aaagattttg aagtagagag acaaaaggat atttataact
     9841 atgtaaaaaa taaatataat agtatagaaa tagaaatcaa tgattatcat caactatccc
     9901 ccataaaaag tgttgcaatg cttattttaa tgggagatga agaaaattta gaatcaagat
     9961 gtagcaaatg tcctagaaaa tgcttttaac tagttttcaa gactaagatt tgttttgtgt
    10021 atgaattgca ataatttttt tggtcatttt atgtataaac taaaaaaatt attgcaaagt
    10081 aatattctat ttaatttgaa cagacttaca aactatagta gtttctttaa ttcaataagt
    10141 gtatttattt cataagttgg tatatgatta gtataattta tactgttatt ggaatttaac
    10201 caacaagtat ctattcctga atttattcca cctaagacat cagaggttaa gctattccca
    10261 atcattaagg cagaagattt atcatgacaa ttaaactttt tgagagcgta tttaaatatt
    10321 tcaggatttg gttttgatat tttcaactct tcagatacaa ccattccatc aatatatcct
    10381 tttatttttg aattttcaag tcttttcttt tgaactgcag aaattccatt tgttattata
    10441 acaatagtat actttttttt aagatattgt aatatatcga ttgcacctgg aattaaaaat
    10501 gtacattcac ctaaaaaatt taagtacatt ttgctaagag tttcagaatc aacatccaaa
    10561 gatattttat ttgcaaaaag ttcaaatcta agtaatttaa gttcattcaa agttataata
    10621 tttttttcaa ggtcgagcca aagcttatca cttatatttc tatatgactc tatataattc
    10681 tcaaaattaa attctaaatc aaactcagat agaagttttt taaaagcatg tgattcactt
    10741 ttcttaaaat caaataaagt atcatctgca tcaaaaaata taaatttata tgtcttcatg
    10801 gtttttactc ctttacaaga tgactgcaaa aattacttta ctttcactat ttgagtcatt
    10861 ttgccattta tgctttgttc ctaaaggaac ttttacacta tctcctttgg acaaagtaaa
    10921 ttcatcatca tccataaaaa gtttaacttc tccctccaaa acatatgcaa tttcatctcc
    10981 attatgagta atcctatcaa tacaagaaga agtatttgga gctaaatcca taattgcaaa
    11041 ttctaaatta ccactagagt ctggaattaa aatctcatat ataacttctt tgcttccagg
    11101 taaaataact ttttttctat tatcatgtct tactatcaag ttttttttat ctacttcaga
    11161 tacaaaaaat gtaaataaag gaacgtttag agaagaggct atagatttta atgaatttac
    11221 agatggatta gcaagacctc gttctatttg gctcaataat gatggtgtaa catcagctag
    11281 ttttgctaaa tctcgaatac ttaagttttg tttttttcga acttcagcta ttttttcccc
    11341 taaatttaaa tcattcatat aaatatcctc tctttttaat ttaaaaaata ttaacttaaa
    11401 ataatcaagt aaaatgatta tgtagtatga aattattata acatatagaa ttaggttttg
    11461 aaaaaactat gttcaatata attaaataaa ttaaaatata attgacaaag tggatacaga
    11521 atacttaaaa tatatttaaa taaaattaag caaaagatag ttaatttatg ataggagggg
    11581 acattatgca aataacgact tttggagcta tatttggttt attaatagca attattttaa
    11641 ttataaaaaa atttcaagct gtatatagtt taatgttagg agcatttatt ggaggattag
    11701 ttggaggagc aaatataaca caaactgtag attttatggc aaatggagct atgaatatat
    11761 caccatcaat tcttagagct ttggcatcag gtgtattagc tggaagttta ataaaaacag
    11821 gagcagtaga taaaatatca gaacagattg taaaaatatt tggtgaaaag agggctttat
    11881 tttcgattgc catttcaact atggtacttg ctggagtagg cgtaaatttg gatgtatcta
    11941 taataactgt agcacctata ggattatata taggtagaaa attaaattat tctaaattgt
    12001 caatactatt agctatgttg ggagggggta aagcaggtaa tataatatca ccaaatccaa
    12061 atactatagc agtagcagat aatttttctg tcaatctctc tagtgttatg atggcaaata
    12121 taattccagc aattattgga gttgttatta cagttatttt agcaagtata ttaataaata
    12181 aaggaaataa agtacaatca tatgaaattt tagagcaaag agaagattta ccaagtttat
    12241 ttaaatcatt atgtggtcca attatagcta tatttctatt gtttttagga aatgtatctc
    12301 ctatagttat agacccaatg atagcccttc caataggagg aatcgtaact ctaattgtca
    12361 caggaaactt gaacaattca agagaatatt tagcttttgg attatctaag atgcaagggg
    12421 tatgcatatt attacttgga acagggacta tcgcaggaat tatacagatg tctgaattac
    12481 aacagagtac aataggtgct ttacaatttt taaatatgcc acaattttta ttggcaccag
    12541 tttcgggtat acttatgtca ttagcaacag catcatcaac agctggtgct actatagcat
    12601 catctacttt tcatgatgct ataataaatg gtgggctatc accgatttca ggtgcatcaa
    12661 tagtaaatgc tgggtctagt gtattcgagc aattaccaca tggctcacta tttcatacta
    12721 gttcaggtag tataaacatg gatataggtg aaagatttaa gttaattcca tatgaagcat
    12781 tgataggaat agtaatgact ataatttcaa catctattca attagtttta taataaatat
    12841 aaatatttaa tgaaaaaagt aggattttgt tattaaatat tgaatttatt atagtaaaaa
    12901 agaagattgg agttttaaag tgaataaaaa aaagattgtt attataggga ttatttattc
    12961 atttttagta gtattttcac ttacaaatat gtatgtaaat atggagtata atttaaatgt
    13021 atttgaatat attaagaagt cacttccatt tacagaagaa gaaaaaaagt ggttagaaaa
    13081 acacaagaat ttgatttaca gctctgacca aagttcacct cctttgagat ataagggtaa
    13141 ggaagatgga cagtataagg gaattattgt tgatttaata aattctctgt caattcaaat
    13201 tggtagagat ttttatttta aaccaaataa ttggtggaaa gaatctttcg taaattcaat
    13261 agatgatagt ataaagtttt ttgacttgat tccatcaaaa gaaagggcaa ataaatttat
    13321 atttactgac ccaatataca ctttgagtgc aaatatattg aaagataaaa agtcccaaga
    13381 tataaatagt tacatggatt taaaggggaa aacagtagca attccagaag gtgattattc
    13441 tataaatttt ttaaagcaaa aaattcaaga tataaacatt ttattgactc cagatataaa
    13501 gactggagtc aaccatttaa tgtctggaaa agtagacgca gttgttggtg atgaaccagt
    13561 attgcgatac tatataaata attatggact ttcaaataaa tacagtgttt taagtaatcc
    13621 tatatatact aaaaaagcag ttcttgctgt acctaaacaa tatgaagagc tggttagtat
    13681 tttaaataaa ggcattttta aacttcagaa aaatggagtg tataaagatt taaagaaaaa
    13741 atggtattct acgtataatg aagttgatga tatattgtat gagcgaggaa ttgtgcctag
    13801 tatttatctt tttataggta taattttaat atcaatttat gttttttata gctatacata
    13861 tttgttaaag atagaaatca aaaggaaaac agaacaggta atagaaaata aaaaaacttt
    13921 agaggcaact tttaatagta ttacagatat aataatgctt gtagatgaaa ataacaatat
    13981 agtagaatcg aacaaagtac tatatgactt tatgggtgaa atgagttata agatagcaga
    14041 tttgattagc atgataaaag gtgttattaa aaataccttc tctgaaaata ctaataaaac
    14101 tagtgaaata gaaatataca ataaaatttt aaagataaac acttttcctg tagagtataa
    14161 aaaaaataat actgagtata tagtagttct tattaaagat ataactaatg aaaaaatagt
    14221 agaggcgaaa ttacttagag aaaacaaaat gatatctata ggacaattag cttctggggt
    14281 agcacatgag ataagaaatc ctttgggtat aataagaaat aattgctatc tattaaaaga
    14341 taacgttaca atggaagaag tgaatgattg tgttaaatca atagaaagta atgtagatag
    14401 ggcaagtaat ataataacca acttgttaaa tttcgcaaga atatcagatg ataatttaga
    14461 acatataaat ataagaaatt ttattgaaaa tatagtgaaa cttcaatata aaatgttaca
    14521 gttaaagaat gttgaaataa aaattgactg tgaacataat ttagtatgct atataaatgg
    14581 agaatcgtta aaacatgtat ttataaattt aatttctaat tcaatagatg caatacatca
    14641 agatggaaaa attattatat actgttatga aaaaaatcat tgtttattta tagattttaa
    14701 agataatggt gaaggaataa aagaagatgc cctaaaggac atatttaatc ccttctatac
    14761 aacaaaacca attggtgagg gaactggttt aggtttatat attacttata atgagattaa
    14821 aaagaataat ggagatataa gtgttgaaag taaattgggg gttggaactt gttttcatat
    14881 aaaaattcca ttaaataaag aggtgactat ataatgaaaa tactattagt tgatgatgag
    14941 ctagaatatg gagtggtaat gaaaaaaatt ctacaaaaga agggctatct tgtagatata
    15001 gcactaagtg gtgaagaagc tataaatata attaaaaaag ataaaaatta tgatttggtt
    15061 ttatctgatg ttatgatgaa aaatatggat ggagtacagc tattagatag aattaaatct
    15121 attaatagag atatagaggt tatattagtt acaggatatg gaagtattga aaatgcagtt
    15181 gatgctatga aaagaggagc tttatcttat tttataaaaa gtaatcccat tgaaaattta
    15241 ttagaagaag ttgaaaaggt aaaggcaagt aaaatatctg tttctgtaca aaaaaataat
    15301 tttgaattta ctttggaatc taaaaataga gattttaata atatcataaa aatagcaaaa
    15361 aaagctgctt gtaaagatgt taatatatta atcttggggg aatctggtgt aggaaaagat
    15421 atattagcta gatatataca tagtataagt ccaagaaaga atgagatatt tgtacctgta
    15481 aattgttgtt ctttttcaga aaatctattg gagtctgagt tgtttggtca tgaaaaaggt
    15541 tcttttacag gagcagtaga tagtagaaaa ggtagatttg aactttcgaa taaaggaact
    15601 ctatttttgg atgaaatagg agatatacct ctaaatgttc aggtaaaact tttgaggaca
    15661 cttgaggaca aatctataga gaggatagga agtaataaat ctattaaagt tgactttagg
    15721 ttaatttgtg ctatgaataa agaaccaaaa gtggaaatat caaatggaaa tataagagaa
    15781 gatttttttt atagaataag tacaataaca ataactatac caccactaag aaagagaaga
    15841 gaagacttaa ctactcttat agaatttttc ttaaataagt accaaattga acatgataaa
    15901 aagatacact cgattgataa ggaagtaaag gattttttat taaattacaa ttatccagga
    15961 aatataagag aattaaaaaa tataataaat agacttgtag ttttaagtga agaaggcaat
    16021 ttatcaaaag acaatttaaa tttaatatca aataatgtat acatagatga taaaataagt
    16081 attagacctt taagagaaat aagaaaagag tttgaatgtg agtatattga aaaagtttta
    16141 tctttgtgtg gaaataatat ttcaaataca gctaaaaaat tggagattag tagaagacag
    16201 ttaaccaata aaatatcaga gtataatatt aagtgagaaa aaaacttctc attaagtgag
    16261 aaaaaaattt ctcaaactgt attaaaagat aaagagaaaa aatctgttta tcttttttta
    16321 ttataatttt aaaaattaat atttacaata attattggta aattcaatac tggtgtaaat
    16381 taaaaatgaa aatttctatg acattaatac atagaatata tattttaaat aattggcata
    16441 gaaaatgctt tatatattaa taaacgtaat aaatattttt aattgcgatt aaatatatta
    16501 agggggattt tcatatgaag ctagagctag ggaatatatt tataaaaaat gttgaatttg
    16561 gagaaaagac agaagtaaaa gatggagtac tatatattag tagcgaagaa atagaaaaga
    16621 tagtattaga agatgaaagg attgtaagtg taaatgttga acttgcaaga cctggcgaat
    16681 caataagaat agctccagtt aaagatgtaa tagagcctag agttaaaatt ggagatgaaa
    16741 gcaaaatatt tcctggaatt ataaataaag tgaagactgt aggaagtgga agaacccatg
    16801 tacttttagg agcttgtgtt gtaacttgtg gaaatatagt tggatttcaa gaaggcgtaa
    16861 ttgatatgag tggtcctact gctaaatata cacctttttc aaaaacaaat aatatatgta
    16921 ttgttataaa agcaaaggat ggtatagata ctcatgatta tgaagaagca gctagaattg
    16981 caggattaaa aataggtgca tatgtaggtg aggcaggaag agaagttgag ccagatgaag
    17041 tcattgtata tgagacaaag ccattattaa aacaagtaaa agaatatcca gacctgccaa
    17101 cagtagcata tgttcacatg ttacaatcac aaggactttt acatgacact tattactatg
    17161 gggtggatgc taaacagata gtgcctacat ttatgtatcc aactgaaata atggatggag
    17221 ctataatatc tggaaactgt gtagcaccat gtgataaggt tacaacattc catcatttaa
    17281 ataatccagt tatacatgac ttatataaaa gacatggaaa agatttaaac tttataggag
    17341 ttattttgac taatgagaac gtattcttag tcgataaaga gagaagctct gatatggtag
    17401 ctaaattaat agaattttta ggagttgatg gagttctagt gacagaagaa ggttatggga
    17461 atccagatac tgacctaatg atgaattgta gaaaatgtag tgaagttgga gcaaatgtag
    17521 ttttgataac ggatgaattt cctggaaaag atggcaagag tcaatctata gcagacgcta
    17581 ctaaggaagc agatgcagtt gtttcttgtg gtcaaggaaa tctagtggtt catttcccag
    17641 caatggaaaa aattatagga actttagatt atgttgaaat gatgataggt gggtataaag
    17701 gatgcttaaa tgaagacgga agcatggatg cagagctaca aattataata gcatcaacta
    17761 tagcaaatgg ctataatcat ttgacagcaa gatattatta aataagtatt tgaatataaa
    17821 gttctgttga tgtaatcgtt ggtctaattg atgtaataac tataaagtga tttggaggat
    17881 taagagatga gtaaaatcag agtggttcat tatataaaca atttctttgc tggtataggt
    17941 ggagaggaaa aagcagatat tccgccagaa aaaagggctg gtgtagtagg acctggaata
    18001 gcatttcaga gtcaatttaa tgatgatgca gagataatct caactgtaat ttgtggagat
    18061 acgtattttg gtgaaaatat tgagagtgca actcaagaac ttttaaaaat gataaaagaa
    18121 gaaagccccg atttatttat tgcaggccca gcatttaatg caggtagata tggagtagct
    18181 tgtgggacta tttgtaaggc agttgaagaa gagttaaata ttcctgtagt tacaggaatg
    18241 tataaagaaa atccaggtgt agatatgttt aaattagatt tgcatatagt gtctacagga
    18301 aattcagcag caagtcttag aaaggtagtt ccaataatga cgaaactagg tcttaaattg
    18361 gttagaggtg aggaaatagg acctcctgaa gaggaaggat atataatgag aggaataaga
    18421 aaaaatttct tccatgaact tagagggtct gaaagagcaa ttgatatgtt ggttaaaaag
    18481 atgaaagggg aaagctttga aactgagtat ccaatgcctg aatttgatag agttacacct
    18541 atgaaacctg ttaaagattt atctaagata aaacttgccc tagtaacttc tggagggata
    18601 gttccaattg ataatccaga caaaattgaa tcttcaagtg ctactaaata tggaatttac
    18661 gatttaacag atatggattc tatgtcaaat aaagaattta caactataca tggggggtat
    18721 gacagagcat atgtccttga aaatccaaat ttagttgtac cattagatgt agttagagag
    18781 cttgaaaaag aaggtgtaat tggagaatta gtagattatt ttataactac tacaggaaca
    18841 ggaacgagtg ttggcaattc taagagattt ggagaagagt tttctaagaa attgttagaa
    18901 gatgatgtag atgctgtaat tttaacttct acatgaggta cttgtactcg ttgtggagca
    18961 acgatggtaa aagagattga gagagctggt atagcagttg ttcatatatg tacagtagta
    19021 ccaatatcac ttacaatagg agctaataga attgttccag cagtagggat accatatcca
    19081 cttggaaatc ctatgttagg agaagaggaa agtaaaaaaa ttagaaagaa tatagtttta
    19141 aaagctttaa atgcactaac agaggatata gaagaacaaa cagtatttga ataataaaaa
    19201 tataatatag aaataaaaaa agatatcata aaatagtttt tgaaaattat tttatgatat
    19261 ctttttgcta tggatggaat gaagaaagca attaagtaat aaaaatattt atagtaaaac
    19321 agccaattaa agtataaaga atgaaaaaaa taaatagaag attatacata aacaaaaaaa
    19381 gagattttaa aatctctttt tattatatat taattatttt ttaaatttat attttttgca
    19441 tcagattttc taatagctaa gtcaaaccct ctaaatctaa tcactatagg gtcattaaga
    19501 ggagctatct ttttcacttc aacttcagtg ccatctatta gtcctaaggc tagaagtctc
    19561 ttcataagtt tttcatttcc agctatattg tctataattc ccttttgtcc aagttttaag
    19621 ttgtaaacag tcattattaa cacctcctaa tgaaaatcat tatcaaactt tatatttata
    19681 atatataaca aaaagagtaa attgtcaata tataacaaaa aataaacata ctaagttagt
    19741 atgtttataa aaaatagtat acatgggata ctaatagcat ctagcaaaaa accacttact
    19801 aatggtacaa ccaaaaaggc ttttggagaa tcaccatatt tttgtgtcaa agaactcata
    19861 tttgccaaag cgttcggagt agctcctatt ccatgtccaa tcaaaccact tattatgatg
    19921 gcagaatcaa aatcttttcc aagtactcta aagcaaataa atattgaaaa taggattata
    19981 aagattactt gaaaagttac tattaagaga ataggaggta ataatgtaga taattcaaat
    20041 aaatctatat tcataagtga taaagttaaa aaaattccca atgaaagttc ttttaaaaaa
    20101 tctataagtt taaaatttaa ttctaatata tttatcttat catttaaatt tctaaaaata
    20161 acagctgaaa acatacaagt tacaacagag ggtaatagta tattaaacgt tatatagaag
    20221 catctactta taatttctcc tgtatttata caaataaata tgagaagaac ctgttccaaa
    20281 aatacagatg gtgttattgt ttcggagaag ttattatttt ttatcctatt cttatttcta
    20341 aaaaatttac ttccaaatct ctttagattt ctattatatc taggaatcga caagcctata
    20401 tttgatgatg gttttaattt atatttattt attagaaatt tagctacagg acatcccaat
    20461 ataccaccca taataagtcc tattgttgca gcagataatc caaccccaac tgctccttct
    20521 ataccgagat tttctattgt aagtccaaat gcagcagaat aaccatgacc accttccata
    20581 gatacatttc cacacataag ccctaaaaga ggttcaagtt ttataatctt tgatattgta
    20641 atagctaata tattttgaca aaatgccaat actccacaca aaatccaata tctaaataat
    20701 acactaccac ctttctttat aagatttatg cttatttcaa gtcctatagt tgtaaaaaag
    20761 aaggttataa aatttggcat taaatttcca cttatagata tatctgctat attaaaatat
    20821 ttaaaaaata aatttagaaa acaaaataat agacctccta ttactggtgc aggtatacag
    20881 aaattattta aaaagttaac tttattcttc aataaattac caattaaaaa gaagaatata
    20941 gatagtgtca aggtttgcac tatatcaaaa cttaaggtaa tcaaaataag tcctcctttg
    21001 taattcaaag ttttaattat ataacttaaa ttcttatgta ttgaaatttt attatcaaat
    21061 gtatattaat aaatgtatat taaaaagaat cagtatatat aattagatag tcataaatta
    21121 aattaaaata ttttaatgag atgtgacttt tttagaatga aaaaccgtag tatattgata
    21181 taatagaaat aagacttaaa aatctgttga tagtattatt tataagtaca gtaggaggat
    21241 aaaatgtttg aaaatttacc taagatagat ttacattgcc acttagatgg aagtgttaga
    21301 gtagaaacca tgcttgatat agctattaaa gagaaaatag atttaccttc aaataatatg
    21361 gatgaaataa aaaaattagc aaaggtttct tttaattgta cttcactaga tgaatattta
    21421 gaaaaatttg atttaccctt aaaagtaatg caatctaagg aaaatttaaa aagaattact
    21481 tttgaacttt tggaagatgc aagtagagaa aatgtaaaat atattgaaat tagatttgca
    21541 ccattacttc acacccaaaa aggtatgagt gttaaaaata taattgaggg tataattgaa
    21601 ggaattagag aagctgaaag tatttatgat ataaaaggca atttaatatt aggttgtatg
    21661 agaacaatga cctcaaaaga ggcattatta gtaattgaag aagggaaatc atttgtaaat
    21721 aaaggtgttg tagcagtaga cttgtgtggt ccagagaaag agggattttg taaggaatat
    21781 aaagatgttt ttaaattagc aagagaatat ggatataaag ttacaataca tgcaggagaa
    21841 gctgcaagtg gagaaaatgt attggatgct ataaatattt taaaggcgga taggattggt
    21901 catggggtaa aaattaaaga ccataaaaag gcgtataatt tagttaaaga taaaaaaata
    21961 ttgcttgaat tatgtccaac tagtaatgta caaactaaga cagtagattc ttatgaggta
    22021 catccatttt atacttttta taaagataat ttacatgtaa gtataaatac tgataataga
    22081 actgtatcag atataaattt aagttcagaa ctaaatgtta tatttgatac ttttaaacta
    22141 ggattagaag actataaaat tatttataga aatgcagtag aagctagttt tgcagataaa
    22201 gaaactaaag agtatttgaa tagtttaatt taatagataa aaatatttaa actttaaatt
    22261 aagctattaa attgacaaaa atacattgta ttaggagaaa aaatgtgaaa gattatgaag
    22321 atgttgttta tattaaacta gtcaaaaaag aacttgaaga atggaagaaa aatatattaa
    22381 agaaaccttc tatatttgac aaagcatcaa agggtataca gaataaattt aatggagtat
    22441 tgccagataa atatcatgaa ataattactt cttctataaa agtaatgaca aaaaccgtac
    22501 tgtttggata taaatatgct acaaagccac cacttagaaa tatgtcatta gaagaaagag
    22561 aaaaattagt tttagaaaaa gctaaaactt acaagaccac agcaatgatt gaaggggcag
    22621 gaacaggggc aggaggaatc ctcttaggtt tatctgattt tcctttgctt ttaggtatta
    22681 aagtgaaatt tttatatgat gtagcttcaa tatatggata tgatgttaaa gattatagag
    22741 aaaggattta tatattaaac atattacagt tggctttttc tagtcaacag catataaaca
    22801 aaacttttaa attgatgaat aactgggatg aatatgtaaa tagtctacca gaagacatca
    22861 atgattttga ttggagaaca tttcaacaag agtatagaga ttatttagat ttagctaagt
    22921 ttttacaact aatgcctgga ataggagcat ttgtaggatt ttatgtgaat ggaaaattaa
    22981 ttgataaatt atgtgaaact gctgtgtctg cttatagatt gaggattcga gagcttaagt
    23041 tataagagaa attataacat aaaaaattag ttatacaata tatctatatg tatctaattt
    23101 tgcattgata aaaacaatta tggataaaag gtaaagcatg atagaatcta atgtagaggt
    23161 ttgtatttta atggaaaaat ttagttttta ttgcttaaga tacaaattga attacgaaga
    23221 gaagtaattg ggaggaatat tttcatgaca aatttgccaa aacaatttga tagcttcaat
    23281 gaagctagac aaaagggatt tataaatgca aaggaactta aggaaagtgg gaaaaagatg
    23341 gttggagttt tctgtacatt tactccagtt gaaattccaa tggcagctgg agcaactgta
    23401 gtaggtgtat gcggtgttag tgaagagcca atacctgatg cagaaagggt gcttccaaga
    23461 aatctttgtc ctttaattaa atcaagctat ggacatgcaa taacagacac ctgtccttat
    23521 ttctattttt ctgatttgtt gataggagaa accacttgtg atggtaagaa aaaaatgtat
    23581 gaagaacttg caaaagttaa gccaacatat gtaatgcatt tgccaaatac ttcaaaagga
    23641 gaatttgcct ataaactttg gaaagatgaa atgataagat taaaagaaga ggttgagaaa
    23701 tctttaggtg taactataac tgaagaagat ataagaactg ctataaaaga taaaaatgaa
    23761 gaaagagaac ttttaaaaga gttttatgca cttggtaaat tagaaccatc tgcacttaca
    23821 ggccttgagt tacataatgt tttatatcaa gcaggtttta aatttgaccg tgcagaactt
    23881 aaacattctt taagaaaagt tatagatgat atgaaagaaa gatatgaaaa aggtgaatgt
    23941 cctgttcaaa aagataagcc aagaatattg ataacaggtt ctcctattgg tggaataagc
    24001 gaaaaaatag ttaaaacttt agaagatgca ggggcatcag tcgttgcata tgaactttgt
    24061 ggagctatta gatcaaacga tttattggtt gatgaagaaa tagaagatgt atatgatgct
    24121 ttaacacaaa aatatataaa tataggttgt tcttgtatga tgaataatga caatcgtatt
    24181 gaattattag atagaataat agatgaatat aatgtagatg ctgtaataga tgtagtttta
    24241 caagcatgtc atacatttaa tatagaaagt tatagaataa gagaatttgt tacaaaagaa
    24301 aaaaataagc catttatgtc tcttgaaact gattattcaa aatcagatac tgaacaatta
    24361 agaactagat ttgaagcgtt tgtagagatg ttgtaggaat aaataaaagt aggaggaact
    24421 atgtttagta taggagttga ctcagggtca gtggcaacaa agggtgtgct atttgatggt
    24481 gaaaaaatta ttaagaaaat aattatacca actggatgga gccctaaaaa tacatctaaa
    24541 caggtctatg agttgctaag tagtgaaatt gacaaaaaag atataaaaaa ggtggtaggt
    24601 acaggttatg gaaggggagt aatggatttt gctgataaga aagtaacaga aataacttgt
    24661 catactagag gtatttactt tttaaacaag aacataagga ctattttaga tgtaggtggt
    24721 caagatagta aagttataaa tttagataga gatggaaatg tatttaactt tataatgaat
    24781 gataagtgtg ctgcaggaac tggtcgattt ttagagatta cttctaacct tttaggaagc
    24841 gatattgaaa gtatagatac attagctaaa ggatatgaac cagttaatat atctagtatg
    24901 tgtacagttt ttgctgagtc tgaaattgta agtcttctag ctcaaaacat ttctactgga
    24961 gaggtagcag cagggatact taaatctatt gcaaataaat ccacctcaat gttagcaaga
    25021 ggtgaggtaa tagatgaagt tgcatttact ggagggcttg caaaaagtaa agaactagta
    25081 aaaatgatag aagaaatact gggtaaaaaa atttttattg cagaagatac tcaaataata
    25141 ggagctttag gagctgctgt aataggattt agataaaaac atataaaaag aaaatgaaaa
    25201 gctgaattta tcataaataa gttcagtttt ttattttatt ttgtttattt ataaaggaaa
    25261 actttggtat aattgattta aatgattgta tatttgtaaa tacttgatat ttagtcatat
    25321 ataacattag aattttaaaa attagctcaa aataaggggg agaaagcgtg aaaaaattta
    25381 cttcaaaaaa agtagcaagg tttgttgcac tgtttttaat atctatttta gtaatgtcaa
    25441 caattcctgt gtcagctgac aatagcaata cattgaatca gataaaaaaa gcagaataca
    25501 gtgatactta taaacaatat ctaaatgatg ctaaaaatgg acgtacagaa aaatataatg
    25561 gaattatacc aaatccatat cagttagaag gtactcaaat tcaaagtaaa ggaagaacag
    25621 ctccaacatc ttatgaccct agaaaattag gtcttatgac atcagtaaaa aatcaagagg
    25681 atttaggaat atgctgggat tttgctgcaa tggcaacctt ggaaagtttc ttgaagttaa
    25741 ataattatgg agactacgat ttatcagaag aacacttaag atggtgggct tcagatggtg
    25801 aatatggatg gtctgtaaat gatatggatg gtgctttaaa ttatgaagct atgggatatt
    25861 taacatcttg gtcaggtcct aaattagaaa aagatatccc atacaatggt agagtatcta
    25921 aagcacaagg agcaaaaaaa ccaactaata tgaatacagc acctactgtt tttaatgtga
    25981 cagatgctgt gtgtgtaagt aatgacataa attctacaaa aaatgctata ttacagtatg
    26041 gagcagtaac atctggttat tatgaagata ttaaatatca aagtgatgac caaaattcat
    26101 attattgtcc aacaaaaagt tttaatacaa atcatgctat ttcaattgtg ggttgggatg
    26161 ataactattc taaagataat tttaatgcaa atattagacc atcaaaaaat ggtgcttggt
    26221 taataaaaaa tagctgggga gattataatt ctgagggtgg atatttctgg atatcttatg
    26281 aagacaagac acttatgtct gatatagata atttttcaat caaaggtggt aaaaaaccaa
    26341 atgatgatga gaagatgtat caacatgact atgcaagtat tgtaccttta atttcaaaaa
    26401 agataacagc tgcaaatgta tttgatttca atagaggcga tgaaactcta aaatctgtaa
    26461 tgttcttaac tgaaagtata ggagctaagt atgaagtatc ttatgctcca gtagtaaatg
    26521 gtatacctca agaaaacaat atgaaaaaat taaaagaagg tactgcccaa tattcaggat
    26581 acataacagt tccaattgat tcttttaata taccagaagg aaagggtgca attgtagtca
    26641 gtatagaggg gaaaaatgga gagtcaacta taggttctga gtcaaatgta ccaggatatg
    26701 atacatttaa agcaaaagct aatttaggtg aaagttatat catagataat actggaaaat
    26761 tctttgatat aaatagagat agtaacttct atccgtgtaa ttttactata aaagctgtta
    26821 cagaaaagtc ttctggtgag agtatcccga atgagtcttt aattggtagt gatagatatg
    26881 aaactgctat caaagttagt caaaatggtt tcaattcttc agaaaatgta gttttagtaa
    26941 atggcagttc aatagtagat gcattggcag caactccttt tacatctgct ataaattcac
    27001 caatattatt gacacaaaaa gaagctttaa attcaaaaac taaagctgag atacagagat
    27061 taggtgctaa gaaagtatac ctaataggcg gagaaaattc tataagtaaa gaaattgaac
    27121 aacaattaaa aagcttaaat atatcgatag agagaatatc aggaagtgat agatacaaga
    27181 caagtttatt attagctcaa aagttaaatg gtataaaaaa tgtatctcaa atagcagttg
    27241 taaatggagt gaaaggatta gctgatgcaa ttagtgtagg tgctgctgca gcagaaaata
    27301 acataccaat catacttgca aatgaaaaga gcgaattgca aggagctgat gaatttctaa
    27361 attcattaaa tatagagaaa tcttatatta ttggaggaac agcatcccta tcaaacaatc
    27421 tagaaagtaa gttaaaaaat ccaactagat tatctggtag tagtagagac gaaactaatt
    27481 caaagataat agataatttt tataaaaaag atactttaaa aaatgcattt gtagttaaaa
    27541 atggaatcaa gaatcaaaat gatttaatag atggattggc agttggtcct ttaggagcaa
    27601 aaactgggtc accagtaatt ttagttggtg ataaattagc tgatagccaa aaagaagttc
    27661 ttaaaaataa gactttagaa aaagtaactc aagttggtgg aggagcaaat aaaaatgcat
    27721 ttaaagagtt agtaaaatta aaaagtgcta aatagagtac ttaagtatta acatatatta
    27781 gtgtgtatta gtaacttatc ttaataaaag ctgaaaataa ttactttaaa ttattgataa
    27841 ataagataat caatgattgt aaagttttgt tttcagcttt aaatttatgt agtataagtt
    27901 taaaaacata aaaaagggtt actatatata gaaatataga aaatcttata ttttatatat
    27961 aaataaccca tatttaatta actttttata gtgttctaat agatattatt gaacttaatg
    28021 gtaaatataa tgtgtaagtt tcatttaaag ttggattaga ttcatctata tatcttgagc
    28081 aaagtgtaat tgataagcta tctgcttcta taatttcacc atatagagga caactgctaa
    28141 agtaagaatg tacttcaaca tatttattta agttttcttg aacataatcg taaaaacggt
    28201 tttttgtagt agtagccata aaatcaactc ctttattttt tattaaagtt gaaactttta
    28261 aaaagataaa tatgagtact tctattagaa actaagttaa aaaataagca aatagatatg
    28321 aaaattagaa taatatatta tttatatctt aattataaca ctagataata aaaaatgcta
    28381 tataaaattc agaattttcg aaagttttta agcttatttt taaatatact aataatacta
    28441 ttgacattag atagaaaaag tattatattg actatataaa ttattttagt caaactggag
    28501 gaaaaagatg cctaaatcgt attctgataa agaaagagag tatataataa aaagacttaa
    28561 agaagaagca agattgtgta tggagcaata tggtattaga aaaactactg ttgatgagct
    28621 tgtaaaacgg gtaaaaatac caaaaggaac tttttatcta ttttttcaat caaaagaatt
    28681 gctctttttt gaggtattga gagatataca tgactctata caaaaagaaa tcttatatga
    28741 gataaataga gtagatgaaa gtattacatg tgaacaattg acagatatat ttatgaaatt
    28801 ctataggatg gttgatagta cttcaatttt aaatctcatg ataaatggag aatttgagat
    28861 attggtgaga aagttaccag attctataat tgaagaacac tttagacatg atgactttga
    28921 aattggagag attatatctt gcataccaaa tgcaaaaaat aaagacatag aaagctttag
    28981 tggagcattt agagtagttt ttcttaccat gctatataag cgcgaggttg gaagtaattg
    29041 ttttgaagat gctttaagat taatgataaa cggattagtt atacaactta tggaatagaa
    29101 tttaaatttt gtttgctaat aacttttatt aaaaattatt aacaaacaaa aaaattatat
    29161 tttattgacc atatagtcaa aatagtcaaa aatttgaaag ggtgatattg aatgattaaa
    29221 gttgatgatt tatcatttag ttatacagat agaaattttt tacaaaatat taactttgaa
    29281 gtagaaaaag gggaaatact aggattttta ggaccatcag gtgcaggaaa atctacatta
    29341 caaaagattc taattggaat gataactaat tatggaggaa gtgtcattgt aaatggagta
    29401 gagagtaaaa gacactcaaa taaattttat gaaaatatag gagttgattt tgaatttcct
    29461 agtttatatg aaaagttgac tgcaatagaa aatttaaaat attttgggtc tctatattct
    29521 aaaaaactac tatctattga tgagttatta aaatcagtag gactagaaaa tgaagcaaat
    29581 aaaagggtat ctgagtattc aaaaggtatg aaatctcgat taaattttat aaaagcttta
    29641 ttacataatc cagatatttt atttttagat gaaccaacaa gtggtcttga cccgtcaaat
    29701 agcaaggtta tgaaagatat aattttatca gaaaaatcta aaggtaaaac tattatttta
    29761 acaactcata acatgttaga tgcaacggag ctttgtgaca gagtagcgtt cattgtaaat
    29821 ggtaaaatat ctgctttaga tacaccacat aatcttatta tgtcaaaagg agctattaaa
    29881 gttagatata cttactttga taatggagaa aaaacttctg aatgttttct gaacaatact
    29941 gctaatgata aaaatttgaa tatgcttatt aagaaaaata aacttttatc aatacatagt
    30001 agtgaaccaa cattaaatga tatttttatt gagataacag ggaggaattt acaatgaggc
    30061 taaaaaatct tattttagga gatataaagt ttcaatttaa gtatggtttt tattttttat
    30121 atcttttttt aagtattata tatatttgta ttatcaatgt atttccaact tttatgagag
    30181 aaaaaatagc tataattatg atttattctg acccagcagc aatgggattg ttttttatgg
    30241 gagctattgt acttcttgag aaaagtcaaa gggttttaaa ttccttagct gtatctccag
    30301 taaaagtatc tgagtatatt ttatcaaagg taatttcatt aggagtaata tctagtattg
    30361 tagctatgtt tattgctatt acattaaatt tagataatat tataatatca actataggta
    30421 cctttttttc ttctattata ttttctttgc ttggattgat acttgcaagt aaagcatcta
    30481 gtttgaatca gtttattgtt ttaagtattc ctattgagat tatatgcttt attccaccaa
    30541 tactcaatgt acttcttgac acaaaatcat atgcaaattt gtaccctttt aatatatgta
    30601 tttctcttat tagtggagat aaaaatttta taatgattaa tattttaata tcaattataa
    30661 taataattta ttttataact tattatttta tttgtagcag ttggaaaaag gtaggtggag
    30721 taaaactatg ataaagatta taaatgcgtt taaacaagag tttgaacaaa ttaagagaga
    30781 tgctatgctt tttatagtat gtgtttctcc aatattatgt ggagtattta taaaatttgg
    30841 aataccatta atacagaata tatcacttaa caagttttat tatcaattaa atttagagcc
    30901 gtatttcttg atgtttgatt tacttttagc ttttattaca ccttttatgt tcttttttgc
    30961 ttctacaatg gtcatattgg gagaaattga tgacagcatt tcaagatatt taatagtaac
    31021 tccgcttggt aaaactggtt atcttatttc acgatttgga ataccaggta ccttagcatt
    31081 tattattact atgattctac ttatcttttt ttctctaact aaaatttctt tcttgttaaa
    31141 cttatcaatt tctttattag ttttattaca aggagttatt atatcaatac tagtgatttc
    31201 tttatcatca aataaactag aaggaatggt aattacgaaa ttctcaggtg tttttatgat
    31261 gggtatttta gcaccatttt ttattttaaa taaagcacaa tatattctgt tcttcttacc
    31321 aactttttgg atatcgaagg catttaagga agataattat atctatatgt ttatttcaat
    31381 tgttatatca ttaatatgga ttttattatt atttaaaaag tttagtaaaa aaat
//
LOCUS       DACTGW010000019        30771 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.019, whole genome shotgun sequence.
ACCESSION   DACTGW010000019 DACTGW010000000
VERSION     DACTGW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.019"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            255..719
                     /locus_tag="I9573_000708"
     CDS             255..719
                     /locus_tag="I9573_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAT4795342.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            726..1220
                     /locus_tag="I9573_000709"
     CDS             726..1220
                     /locus_tag="I9573_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAT4795343.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1236..2261
                     /locus_tag="I9573_000710"
     CDS             1236..2261
                     /locus_tag="I9573_000710"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HAT4795344.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2248..4695
                     /locus_tag="I9573_000711"
     CDS             2248..4695
                     /locus_tag="I9573_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAT4795345.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4875..6248
                     /gene="radA"
                     /locus_tag="I9573_000712"
     CDS             4875..6248
                     /gene="radA"
                     /locus_tag="I9573_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAT4795346.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDENIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6251..7321
                     /gene="disA"
                     /locus_tag="I9573_000713"
     CDS             6251..7321
                     /gene="disA"
                     /locus_tag="I9573_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HAT4795347.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7410..8504
                     /locus_tag="I9573_000714"
     CDS             7410..8504
                     /locus_tag="I9573_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAT4795348.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8633..10810
                     /locus_tag="I9573_000715"
     CDS             8633..10810
                     /locus_tag="I9573_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795349.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDMSTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSENGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKTYESVKDNIEKNIDVYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(10996..11841)
                     /locus_tag="I9573_000716"
     CDS             complement(10996..11841)
                     /locus_tag="I9573_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT4795350.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYQDTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSGKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            12058..13224
                     /locus_tag="I9573_000717"
     CDS             12058..13224
                     /locus_tag="I9573_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HAT4795351.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNIVLDTHQYLMLAESDGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            13246..14892
                     /locus_tag="I9573_000718"
     CDS             13246..14892
                     /locus_tag="I9573_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAT4795352.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYIHSSGFDASILHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWRPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNVLGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            14989..16362
                     /locus_tag="I9573_000719"
     CDS             14989..16362
                     /locus_tag="I9573_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4795353.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMFFYKLDKEYSTI
                     LNELNTK"
     gene            16807..17760
                     /locus_tag="I9573_000720"
     CDS             16807..17760
                     /locus_tag="I9573_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAT4795354.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            17798..18760
                     /locus_tag="I9573_000721"
     CDS             17798..18760
                     /locus_tag="I9573_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HAT4795355.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            18785..19771
                     /locus_tag="I9573_000722"
     CDS             18785..19771
                     /locus_tag="I9573_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAT4795356.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            19784..20830
                     /locus_tag="I9573_000723"
     CDS             19784..20830
                     /locus_tag="I9573_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin dehydrogenase"
                     /protein_id="HAT4795357.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYSGKIRKCDVEKLTAKETIVSNNTSKYSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            20997..22727
                     /gene="lpdA"
                     /locus_tag="I9573_000724"
     CDS             20997..22727
                     /gene="lpdA"
                     /locus_tag="I9573_000724"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAT4795358.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEVPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            23145..26093
                     /locus_tag="I9573_000725"
     CDS             23145..26093
                     /locus_tag="I9573_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAT4795359.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            26071..26535
                     /locus_tag="I9573_000726"
     CDS             26071..26535
                     /locus_tag="I9573_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT4795360.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            26648..26950
                     /locus_tag="I9573_000727"
     CDS             26648..26950
                     /locus_tag="I9573_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4795361.1"
                     /translation="MGVTNVTKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            27012..28376
                     /locus_tag="I9573_000728"
     CDS             27012..28376
                     /locus_tag="I9573_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAT4795362.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            28411..28884
                     /locus_tag="I9573_000729"
     CDS             28411..28884
                     /locus_tag="I9573_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795363.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYINFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGDLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLRFRNKKFENKNI"
     gene            29056..29706
                     /locus_tag="I9573_000730"
     CDS             29056..29706
                     /locus_tag="I9573_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795364.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTFKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            29880..>30771
                     /locus_tag="I9573_000731"
     CDS             29880..>30771
                     /locus_tag="I9573_000731"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAT4795365.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEDSIFIG
                     KNQNPYQEVHHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYRKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILE"
BASE COUNT        12510 a         3213 c         5699 g         9349 t
ORIGIN      
        1 taataaagat gagaaggtat atttaaaata taaagtattt aaagttctga aatctaatca
       61 tgaagcaaaa aattagcatt tggttatcaa taaatataat aaataaaaac tcataattaa
      121 tgaaagaata aattcatatc taaatttcta taaaaaattt aaatttatac aatttagggc
      181 tttaaaaatt taaataatag gtgtaaaata atagtcaaag aaagtcaaaa ataaaaaggt
      241 gtgtggtgat attaatggca actatgacag atataattga aaaatttatt aaagatttga
      301 tggaagaaga taatagcata caaattcaaa gaaatgaact agcaaacttg tttagttgtg
      361 caccatcaca aataaattat gtcttaacaa caagatttac aatagataga ggatattaca
      421 ttgaaagtaa aaaaggtggc ggaggatatg ttcaaataga aaaaatacga aaaagtaaag
      481 atggacatat tagagaacta ctaaatgaaa aaataggaag tcagatatcc tataaaaaag
      541 caaaggaatt attagaaggt ttaaaagagt cggatttgat taacgaaaga gaattaaaat
      601 taatattata tgcaatagat gataaatcat tgtgtatgcc aatatatgaa ttaaaagaga
      661 aggtaaggtc taatatttta aagaatataa taataggatt atttagtata gaggagtgat
      721 aatttatgct atgtcaaaaa tgcaataaga ataaagcatc tgtatattat aataagatag
      781 tcaatggaga aaaaacagag atgtatttat gtagtgagtg tgcaaaagag aatacagaaa
      841 tgaattttaa cttagatatg cctttttcaa tgatggatat attttctaat ttaggttttc
      901 aacctaaaaa agaacttgag gaaaagctag tttgtcctaa atgcaataca acatatagtg
      961 agtttaagaa taatgggaga tttggatgta gtgaatgtta taatgcattt agtagtcaag
     1021 taaatccaat gcttcaaaat atacatgggc atatagaaca tacaggaaaa gcacctaaaa
     1081 aatcttttta taagataagt gtagaaaatg agattaaaga actaaaagaa gatttagata
     1141 gagctataaa aaatgaagag tatgaattag cagctcaatt tagagataaa attaaatatt
     1201 taaaaggttc tattgactaa gattggagtg atattatgaa agagaacata gtcatgaaat
     1261 caagagttag gctagctaga aatttaaata actatccatt tccaaataaa ttagataaag
     1321 aatgtgccat ggaaataata gaaaaagtaa aaaatgcgtt tattaattct aacttagaac
     1381 aaaaagaaga atttgatttc tataaaattg aagatttaga ccaatctaaa aaaatgctta
     1441 tggttgaaga acatataata agcccagatt tagcagaaaa tgataaaagt gcagttatag
     1501 taaaaaaaga taaaactata agcataatga taaatgaaga agaccatata aggatacaaa
     1561 ctatatgtga tgatttaaac ttagaatatg catatagtgt agcaaatgaa atagatgatt
     1621 tgttagaatc aagtttagaa tatgccttta acacaaagct aggttatcta acatcttgtc
     1681 caactaatac aggaacagga atgagagctt ctgtaatgat gcatttacca gcattaagtc
     1741 aactaggcta tatggatgaa ctttataaaa tttcatcaca aataggtata gcaattagag
     1801 ggatatatgg agaacgaaca gaagcattgg gaaatatata tcaaatatct aatcaactta
     1861 cacttggaag aacagagtct aatattatag aaaatgtaag tggacttaca aaagatgcaa
     1921 tttcaaaaga aataaaagct agagaaatat tgcaaaaaaa attaggtata aaactagaag
     1981 ataaaatatt tagatcgata gggacacttg aaaattcaag agtaatgagt agcgcagagg
     2041 caatgagcca tctatcaaat ataaaaatgg gaatagaaat gaactatata gataaactag
     2101 atttaagggc tatagaacaa cttatgatag gtatacaacc agcgcatcag tcaataatgt
     2161 ataagagtga tgatgttgag aatagagata taaatagagc aacatatatt agagaaactt
     2221 tggaaaaatt aagaggaggg atgaattgtg aacttcaata gatttacaca aagagctaaa
     2281 aaggcaattg atttagcgtt tgaatctgct aaaagtctag gacacaatat tgttggaagt
     2341 gaacatatac ttttaggtct tttaagagaa gaagaaggta tagctgctaa agttctaagt
     2401 aaagttggat ttacagaagc ttatttagaa ggcaaaatag ttgatatgga aggtaaggga
     2461 gaagaaatct cagaagatat agtattaagt ccaaggagta aacaaatact tgaattatca
     2521 ggaatgtttg caaataaatt aaaaacaaat tatattggga ctgagcatat attattagca
     2581 attattcaag aaggtgaagg aatagctaat aagattttaa attatgctgg agtaaatgat
     2641 aggactttag cccaattaac aattgatatg atgggtatta gtgataaaaa tcaatacaaa
     2701 gcagagaata gttacacggg caaccagaat caagcagaat ctaaagtact tgataagtat
     2761 ggaagaaatc ttacactata tgcaaaacag aataaaatag accctgtaat aggtagagaa
     2821 aaagaaatac aaagagtaat acaaatatta agtagaagaa ctaaaaacaa tccagtgttg
     2881 ataggagacc caggtgtagg gaaaacagcc atagctgaag gtctggctac aaatatagcc
     2941 ttaggaaatg ttccagaaac actaaaaaac aaaactttat actctttaga gatgggttca
     3001 ttattggctg gggctaagta tagaggagaa tttgaagaaa gaattaaaga agttgtagat
     3061 gaagttgtta aaaatggaaa cataatctta tttatagatg agatgcacac aataataggg
     3121 gctgggtcta ctggagaagg ctctatagat gcttcaaata tattaaagcc agccttggca
     3181 agaggagaaa tacaagttat aggtgcaact acaattgatg aatatagaaa gcatgttgaa
     3241 aaagattctg ctctagaaag aagattccaa ccagttatgg tagatgaacc aactaaagag
     3301 gactcaataa aaatattaga aggattaaga gataaatatg aagctcatca taaagttaaa
     3361 ataactgatg atgctataaa ggcagctgta gaattatcaa ctagatatat atcagacaga
     3421 tatctgccag ataaagctat agacttgatt gatgaagctg catcaaaagt aagattaaaa
     3481 gaaaatacac ctccttcaga aataaaaaaa ttagaactag aaatagaaaa tatagataaa
     3541 gaaaaagaag aagcagtaag atgtcaagac tttgagaaag cagccaaaat aagagatgaa
     3601 caaggtctac ttaaaaaaca attagaagat gttagagaaa gatggaacaa gtcatctaaa
     3661 cattcagatt tagttgatgg agaagttatt gctgaagtag tgggcttatg gacaggaata
     3721 ccagttaata aaattcttga agaagaggct gatagacttt tgagacttga ggaaatactg
     3781 cacaatagag ttataggtca agaacaagca gtaaaatcta tttcaaaggc aatcagaagg
     3841 tcaagagcag gtcttaaaga ccctaataga ccaattggtt catttttatt tttaggtcca
     3901 acaggagtag gtaaaacaga attatctaag gcattagcag aagtgcaatt tggagatgaa
     3961 aatcaaataa ttagaataga tatgtctgaa tatatggaaa aacatgctgt gtcaagaatg
     4021 ataggttcac ctccaggtta tgtaggtcat gatgaaggtg gtcaattaac tgaaaaagta
     4081 agaagaaatc catattcagt tatcttattt gatgagatag aaaaagctca tccagatgta
     4141 tttaatattc tgttacaaat tctagatgat ggtagactaa ctgattcaaa aggaagaact
     4201 gtagatttta agaatactat tgtaataatg acatcaaatg ttggagcatc tacaattggt
     4261 agacaaaaaa ctttaggatt tagtatagct aaaggagacg aagaagaaaa atctcaatat
     4321 gaaaaaatga aagaaaatat aatgggtgaa ttaaaacaaa gattcagacc agagttttta
     4381 aacagaatag atgatataat tgttttccac tcactaaatg aaaatcatat atcaaaaata
     4441 gtacttttaa tggcagcgaa actacaagag agattaaaag aaatggatat aaaattagaa
     4501 atgagtgatg aggctgttaa attaatttct aaatctggat ttgacttaga atatggagca
     4561 agacctctta aaagagcttt acaaaaagag ttggaagatg agctatctga agcaatctta
     4621 aaaggtaatg tgaaaaaggg aagtaatgta gtagcaaaag tcaaagatga aaaaatagtt
     4681 tttgaaacta aataatttaa tagagttgca ttttaaaata ttgcaaatat aagttttata
     4741 atctaaacaa aattttaagg gcaatgaatc tataataatt cattgtcctt aattatttta
     4801 tttaatcttt acatattgtc tttcagatat gctaaaatta tagtatagaa taaaaaatta
     4861 aggatgataa aagtatggca aaaataaaaa caaaatatgt atgtcaatct tgtggatatg
     4921 aaacagctaa atggcttggt aaatgtccag aatgtacaaa atggaataca tttgtagaag
     4981 aaatagacca aaaaagcacc aaaaaagagg tctttataat agataaatct tcatctaaac
     5041 cagtcagtat aaactcaata gaaagtaaag aagaagagag gtttacaaca gatataaatg
     5101 aattagatag agtgcttgga ggaggtatag ttaaaggctc tttagtgctt gttggtggtg
     5161 accctggtat aggtaaatca acacttttaa tccaagtatc tagtaatgtt gccaatttag
     5221 gaaaaactgt tttatatata actggagaag aatcagaatc tcaaataaaa atgagagcta
     5281 aaagattagg tattaactca gaaaatttat atatatttgc tgaaaataat ttaagtataa
     5341 ttgaatctta tttagaaagt gtaaatccag aattaataat tctagactca attcaaactg
     5401 tcttcagtcc agaaatatcc tcggcaccag gaactgtcag ccaaattaaa gaaggtactt
     5461 caaaattcat gaaaatttct aaaaaaatgg gaatttcaac gttcatagtt gggcatgtta
     5521 caaaagaagg ttcactagca ggaccaaaat tattagagca tatggttgat actgttctat
     5581 attttgaagg agaaagatat aatacttata gattagtaag agctgttaaa aatcgatttg
     5641 gttcaactaa tgaacttgga gtatttgaaa tgagggattt aggattagta gaacttgata
     5701 atccatctaa aattttaatc tctgaaaaac caaaagatgt agcaggttca gtaataatat
     5761 ctactgtaga aggtacgaga ccaatgttac ttgaattgca agcattagtt tctcctacaa
     5821 gttttggtat accaaaaaga acatctacag gagtcgatta caatagagta ggtatgttat
     5881 tagcagtatt agaaaaacgt gttggtttgc aaatacaaaa tcaagatgtc tatataaaca
     5941 tagttggagg aataaagata aatgaaccct caattgattt agggatagcc atttctgtag
     6001 catctagttt tagaaatata ccgatagatg aaaatatagc tgttacagga gaagtgggac
     6061 ttacaggaga agttagagca gtaagcttta tagaaaagag aatagctgag tgtaaaaaac
     6121 ttggatttaa aaagatagta gttcctagaa gtaattatga tgttgtaaaa gagaccaaag
     6181 gcatagaaat atggccagta gataacttaa gacaagctat aaatatagta cttgggagga
     6241 atcaataaat atggagaatt ttctagataa taaaaatatg ctatatgcat taaaaatgat
     6301 atctcctgga actccactta gattaggtct aaacaatgta ctaagagcta agactggtgg
     6361 attaattgta attgcaacaa acgaagatgt aatgaaaata gtagatggag gatttgctat
     6421 aaatgcagaa tattcaccat catatctata tgaattagct aaaatggatg gagctatagt
     6481 tttaagtggt gatgtaaaga aaatattatt tgctaatgca caacttatac ctgactattt
     6541 tatagaaaca tcagagacag gaacaagaca tagaacagca gaaagagtag caaaacaaac
     6601 tggtgctata gtcataggaa tttcacaaag aagaaatgtt ataacagttt atagaggaaa
     6661 tgagaagtat gtagtcgaag atatatctaa gatatttact aaggcaaatc aggctataca
     6721 aactctggaa aaatataaga cagtattgga ccaagctgta acaaatttaa atgccttaga
     6781 gtttaatgat ttggtaacta tttatgatgt tgcattagtc atgcaaaaga tggaaatggt
     6841 aatgagagtt acaagtataa ttgaaaaata tgtgatagaa ttgggtgatg aaggaacttt
     6901 agtaagtatg caattagaag aattaatggg tacaaccaga atagaccaga aattaatatt
     6961 caaagattat aataaagaaa acacagaaat aaaagaactt atgaaaaagg tcaaaaattt
     7021 aaattcagaa gaactaatag aattggtcaa tatggcaaaa ctattagggt atagtggttt
     7081 ttcagaaagt atggatatgc ctataaaaac aagaggttat aggattctta gcaaaataca
     7141 tagactacca acagcaataa tagaaaactt agtaaattat tttgaaaact ttcaacaaat
     7201 tttagatgca tctattgaag aattagatga ggttgaagga ataggtgaaa taagagcaac
     7261 atatataaaa aatggactca taaaaatgaa acaattagtc ttattagata gacacatatg
     7321 atgaatttta aatgaatttt actacttttt tcttgaatat aatatacact ttggtctata
     7381 atagataagt ataactaggg ggtgaagcat tgataaggaa agtaacaaga atattgttta
     7441 ttttacttgg atttacaata ggtataacaa cctatttaac attaatgaaa gattttgaaa
     7501 tattaacatt tggaaaagaa acatatggtt atatcgcagc tgtagtagca ggtataataa
     7561 tagccatttt aggatatctt atagaaccat gggttgtaaa taaagtaaaa gaaattgcta
     7621 aaatagtgga taaagaattg tcaaaatatc ctcagactga tatattgtta gggtctatgg
     7681 gtcttatagt aggatttgtt attgcgtatt tattaagtgg attagtaaat agaataccaa
     7741 tagtaggtgg aatactatcc ttactattat atttattcct aggatatctt ggaatgaaag
     7801 ttgcgcttaa aagtaaaaat gatttattta atattggaaa actaggaaga ttagcaaatc
     7861 ctattaagga taaagataag gataaagaaa ataaaaaaga agtaaaagcc attcctccaa
     7921 aagtattaga tacaagtgta ataattgatg gaagaatagc tgatatttgt aagactgggt
     7981 ttatagaagg aaaacttatt attcctgctt ttgttttgga ggaactaaga catattgcag
     8041 attcttcaga tgatttaaaa agagtaagag gtagaagagg tctggatata ttaaatataa
     8101 ttcagaaaga attaaatata gaagttgaaa taagtgagag agactttgat gatatagcag
     8161 aagttgatag taaattatta aagcttgctc aggttttaaa tggaaaagtt gttacaaatg
     8221 attataactt aaataaagtt gctcaattcc aaggtgttga agtattaaat ataaatgagt
     8281 tagctaatgc tataaaacca gttgctatac caggtgaaga tatggttgtt caagtggtta
     8341 aagaaggaaa agaggctcaa caaggtgttg catatctaga tgatggaact atgatagttg
     8401 ttgatggagg aagaaaatat atgaatgaga ctataaaagt actcgttact tctgttcttc
     8461 aaactcctgc aggtagaatg atatttgcaa aacctaaaaa ttaatatgtt caaagaaacc
     8521 tataatttgg gctgtttatt ttaaatagat ggtccaaatt tatttattta taacgataat
     8581 gtttaatgta ttcttaaaga tggggattta aatcagaaag cagggagaat ttatgagaaa
     8641 aaagattata tctttcttag caacatttat cataatatta acatcagcaa gtcagtatag
     8701 ttttgcagat gatatgtcaa cacgtggaaa agtaatattt atagatatga atagaactag
     8761 tatgagtaat atgttaagaa taaaatcatt aagagaagaa ctagacaata gagggtatat
     8821 tggcttgatg aatataagag gcgataaagg aagtgatgat agaaggtcat atgctagtat
     8881 gggtgctggt ggtagagcta atgtggctaa tgaagaagat attaattttg aaagctcgag
     8941 taaggataga aatatagttt ttgaatcagc tacaggaaaa agtgcaaaag gtataaataa
     9001 tttaactata aataagtcta taaatgaaaa tttaaacttt ggagagtatg gttctgtatt
     9061 agggtcatta ggacaaagtt tatctgagaa tggattaaaa gcatctgtat tgggtaattc
     9121 agatataatt gaaaatggtc aacttataaa aaatagaaac ttatgtctaa ctgcaatgga
     9181 tgaatatgga agaataccta atggaaatgt agatactata aataaaaaag acttatctat
     9241 gccttatgga atatcaactg attatgataa attgattgta gagaccaaag aagcttacaa
     9301 aaataatgat gtggtttttg tggagcttgg agacacatat agattagatt tatataaacc
     9361 aaatttaaat gaaaaaacat atgagagtgt gaaagataac atagaaaaaa atatagatgt
     9421 atatttaaaa aatatatttt ctatggttga agaaaatgac actgtctaca tagcaagtgc
     9481 atttccaagt gatttagatt ataaaaataa aagaagactt tctccaataa taaaattaaa
     9541 tggagaaggg aaaggtatat tatcatcttc aactactaga agagaaggta ttgtaacaaa
     9601 tttagatgtt ggggttgaaa tattagacaa ctttaatata aaaaatcaaa atatggttgg
     9661 aagaaaatat gaacttatta acagagatga taataaagaa tttttgatgg atgaatatca
     9721 aaagatagta tctatttcaa gcataaggtc tacaatactg aatggttttg taagtatagt
     9781 tttcttgtca tggatagttg ctatgattgc aattttattt agaaagcata tatcaaaaaa
     9841 ttataaagaa acaacatttt ttatattaaa ggaactttta aaaataggaa tagtaatgcc
     9901 actgtcattt atgataacac caataatgaa tttcaaaaca ccattagcaa tatctttagg
     9961 aataattata ataacactta ctatatatct tataagtaaa gttttaataa agaatgattt
    10021 aaaaaacatg ctgtttttta ctggattaac catagtaatt atggttattg atgctgggtt
    10081 tggctcatat ttaatgaaaa gcaatgttat gagttatgac tgtataattg gagctagata
    10141 ttatggagtg ggaaatgagt atcaaggtgt ggcaatcggt agtgcaatat ttacatttgc
    10201 aattttactt acatataaaa atataccaaa gtggtctata atagtttttt ctttggttat
    10261 cttaattaca agtgcatctc caattatggg agcaaatgtt ggaagtgcta tttctgaatg
    10321 tgttgcattt ttactattta tactattgat atataatgtg aagatagact tcaaaaaaat
    10381 tgtattatta ggtatagcag tattatttgt attaggagtt tttgtagcaa tagatatgat
    10441 tttgggaagt aattcacatt taggtatgtt tgtaaaagaa atttacttta atggaccagg
    10501 agaaataata caaacattta gtagaaagat agagatgaat ttaaagctag ctcaaaccag
    10561 tgcatgggta aatatactgt taactggaat aggtgtaatt ttagttctta tgattaatca
    10621 gattagatac tttaagcaac tgatggatga gtatccaatt gtatttaaag gatttatagc
    10681 atcaatagca ggatgtttag taactctttt agtgaatgac tctgggattg tgtcatcagc
    10741 tacagccttt atttatgtaa ttgtacctat gataacatta agtataaatc tcacagcatt
    10801 aaaagaataa gtactgtatg aataatatgt aaatatgaac acattaattt aacttcttta
    10861 tttacaaaat aaagaagaaa gtttataaaa aataaattat aataaaaaag agtttacctc
    10921 aaaattgatt attctaatca aattttaagg taaacttttt ttattataaa gataaataac
    10981 atataacctc atattttagg tagatttttt tcttatatgt ttaggagtgc atttaaattt
    11041 ttccttaaaa attctgtaaa ataattttgt attagtaaat ccatgagctt ctaatatact
    11101 gcataaggta tcatttgtgt taattaagtc tttataaata tgagaaatac gcacatcatt
    11161 taaatactct aagtaagttt gtcccatata ctgtttaaat tttctacaaa aatattctgg
    11221 ctgtaagtgt gctaccttag cgattttgtt aatagaaata ggcattttgt aattaatctt
    11281 tgtatattct aaaactggtt caaattttga caaatcaagt actgtttttt tcatattttt
    11341 attacctaca gatattttaa agtttgtata taattcaaat aaaagttcaa ataataagct
    11401 tgtaaagagc agattagctg caggaggaga gctaagttta atttctctca tgttttctaa
    11461 tataagttta actttacctg aatttttttt atagttattg tcattggcat taaccttaaa
    11521 gtcaaaatag caatttttaa aatcagggat atatttgttt agaaaagaca atggtacttg
    11581 caataatata gaggtatctt gataagtaca tctagttgag tgaataattc cagaattaat
    11641 gaacatacac tcattagctt ttaaattata agtgtaaccc tccatgttaa tttgtaaatc
    11701 accagaagtt atataaatta tttctaatga gttatgccaa tgatttgcta cataactacc
    11761 actatcatca aatagtttga aattaacatc taaattatca ataggttcaa ttatttcatg
    11821 tgaaaaatta tttctgttca taataatatc ctccttataa ttttaaatta taaatagtaa
    11881 atttataatg ttcaatatta aaaagataat atagactaat aaattagata ataaacatac
    11941 ttaagaaaac ggataaaatc aaatataata actgtatatt aaaataactt aaaaaaatta
    12001 ttttattata agtgcaaaat atacctattt aaataagata agaggggagg attttatgtg
    12061 tatataaaag gtgtaaattt aggaggttgg ttagttttag aaaaatggat gacctcaagt
    12121 ttatttgaag gcacagaagc tgaagatgaa tactatttac caagacagct ttcaagagag
    12181 gcatatgaat ctagaattaa gactcatcgt agtgaataca tcacagagag agattttgca
    12241 acgattaaat ctatgggatt taattcagtt cgtataccag taccatactt tatttttgga
    12301 gattgtgaac cattcatagg atgtgttaaa gaacttgata aagcatttgc ttgggcagat
    12361 aagtatgggc ttagtatatt gatagactta catacagttc caggaagcca aaatggattt
    12421 gataatggag gaatatcagg tatatgtagc tggtcacaaa atccagaata tgtagcgttt
    12481 acattaaatg tattagaaag acttgcaaaa cgttatggga tgagacatga attgtatgga
    12541 atccaaattt taaatgagcc tataacagaa agaatgtgga acataatgaa tgttccaaat
    12601 cggtttaagg ctgtagataa ggagatggca agggggagta aacctaattc attagagttt
    12661 ttacgtgatt tttatataaa agcatataag gtaatgcgtc cttatatgag agaggaaaac
    12721 gttatagtgt ttcatgatgc atttgaatta aaagcatgga aagactttat gagagaggaa
    12781 gaatttaaaa atattgttct agatacacac caatatttga tgttagctga atcagatgga
    12841 tgtgaacaaa gtattgatag ctatctcaaa tatattagag aaaattatgc taaagatatt
    12901 ttacaaatgc aaaaatattt tcctgttata tgtggggagt ggagcttatt taattcatat
    12961 gcttgtggta tagatacaaa tggaggtcaa tcacctctaa atgggattga atcaaatata
    13021 gataaattat caaaagacga taaaagagaa ttatatagga agatagcaaa agcacagctt
    13081 gatgcatgga gaaatggaag tggtcactac tattggaatt ataagttatt actagataca
    13141 gttaatgaag aaggttggat aggatgggat agttgggacc taggcaaatg tgtagctcaa
    13201 gaatggtatc ctatagaata ttaaactaaa attggaggaa aagatatgat taaaaaccct
    13261 atacttccag gttttaatcc agatccttgt atttgtagaa agggagatga ttattatctt
    13321 gtagtttcat cttttgaatg gtttccaggt atacctgttt atcactctaa agacttaaaa
    13381 aattgggagt tatatactca tattttgaca gatgaaacaa aaatagattt gaagaagctt
    13441 ccttcatcta aaggaatttg ggcaccttgt ttgacttatt gtgaagaaga agatttattt
    13501 tacatagttt atggaattat gaactctatg aatgcaagat attttgatgt tgataattat
    13561 ttaattacat caaaagatat aaagggagag tggagtgaac cagtatatat acactcatca
    13621 ggttttgatg cgtctattct tcatgatgat gatggtaaaa agtggattgc atcattagac
    13681 tgggagacta gagaaggata tgaaaaacct ggtgttatat gtttagtgga atactgtaca
    13741 aaaaagaaag aaatagtagg atatccaaag agaatatggt cagggggaac tgatagaggt
    13801 tgtattgaag caccacatat aacaaagcgt ggagattact attatataat gtgtgcagaa
    13861 ggtggaactg gatatggtca tagtgtaact atgggaaggg ctaaaaatat atggagacct
    13921 tatgaaaaag attcaatgaa tccaattgta acttcaattc caggcgattt ttatgaaaga
    13981 catgaccctg accatctaaa gcctaaatac tataatccag aatcaaaact tcaaaaatct
    14041 ggtcatggaa gctatataga aacaacatca ggtgaagtat atttagttca tctaacatct
    14101 agaccatttg taccagagtt aaggtgtact ttaggtagag aaacagctat tcaaaaaatg
    14161 aaatggacaa aggataattg gcttcgaatg gaagatgaaa gtaatttggc aaaagaatac
    14221 gtatcagaaa gtaaactaga agaacattta gtttcatcta taccaagttt tgatgacttt
    14281 gattcaaatg tattaggttt acagtactat gctcctagga tttcaccatt atcatttgca
    14341 gatgttaagt ctagaccagg atatgtaagg attagaggtc aagaatcaag aacatcatta
    14401 aataaagtaa gcatattggc aagaaaatta acttcagtat atgcaagaat aaccactaag
    14461 atggagtttt acccagaggt acatcagcat agtgcgggtt tgataatgta ttatgataat
    14521 atgaattata taaatttaag aaagtattat agtgaaacat tgggtcaaag tgcactatca
    14581 ataatacatc ttgaaaatgg agagaagacg gaatttttaa atactaggat accaataaaa
    14641 gatataccaa tatatcttcg tctgtatata caaggtcgta agtcatattt tgaatggagt
    14701 tatgatgaaa aaaactacca aagaatagga aaggtatttg atacaactaa gttttcagat
    14761 gagtactgta aatatggaga atttacaggt acgtttatag gattaacttg tgcagatcgt
    14821 gtaaagcata aacactatgc tgattttgat ttttttgaat atatagcaga tgaatcaaaa
    14881 gatgtcgatt aataaaatca ttactaaaat tttcagagca aaggtaaatc ttatgctctg
    14941 aaataagaaa cattagaagg caaatcttac aaaaaggagt atggtattat gaatacagaa
    15001 gttaatgtta aagcaactac gattagatat gaaaagattt cattttttga aagaatttct
    15061 tatggttgtg gggatttggg gtgcaatatc atatactcag ctatgtcagc atttttgctg
    15121 ttttattata ctaattatgc agatgttagt gcagcagctg taggtagtat tatgcttgta
    15181 tctaggatac tggatgggtt tagtgactta acaatgggaa ttatagtgga tagaactaaa
    15241 tccaaatacg gtaaggctag gccttggatt ttgagaatgg cgattccttt tgctatagca
    15301 gcagtattat tattttctgt tccatcaaat cttggtatca cttctaaatt aatttatatt
    15361 tttattacat ataacttagt ttctactgtt atttatacag caatcaatgt accctatgca
    15421 actctaaatt cacttataac tcaaaaccag tatgaaagag gagtattaag tatatttaga
    15481 atgatattag ccacttgtgg taccttacta ataaatggac ttacactacc tctagttgaa
    15541 tactttggaa ataatttatc agcatggact aaaacattct ttgtatttgg aatagcatct
    15601 ataatggtat tttttataac atttactgga accaaggaaa gagtaaaagc agttaagcaa
    15661 gataggaatg aagtaatacc atttaaaata ggtattaaat ctttatttag aaataagtac
    15721 tggatacaaa taacattatg tttagtttgc atatttattg tatttgcgat aaatggtggt
    15781 tcatccgtat attatgcaaa gtttattttg ggagatgaaa aattatttgg tcctataaat
    15841 atggtttcaa atatatccca aattatagct atgtttatgg ttgctccttt tataaaaaaa
    15901 tttggaaaaa gaaatgtatt aatagtaggt tcaataatat taatatcgtc aaatgttatg
    15961 tttataatag ttggacaaaa ctatatagga gtaatatttg caagtgctat aaagggaata
    16021 ggaagtgctg gaatagcatc aacaatgttt gctattgttt cagatactat tgaatatgga
    16081 gaatggaaga ctggatatag aactgagggg cttataaata gtgcatctag ttttgggttt
    16141 aaagtgggaa atggattagg ttcagcaata ttaggtgcag tattatcaat tggtggttat
    16201 gtaggaacat cagctactca aagtgattta gctatagtat caataaaagt atgttttata
    16261 tacctaccta tatttataac aatattacaa attatcataa tgttttttta taagcttgat
    16321 aaagaatata gtactatact taatgaatta aatactaaat aatgaaaaag tttcttataa
    16381 agtcttaagt ttaattaagg tctattgtaa atgttcccta taaaaactat tatatattta
    16441 tgattattga agaattttta gagaagcgaa taataaaaat ataagaagac tttaaatgat
    16501 gaaatggttg gtcttttaga aatgatttaa taagttattt ttatttaaag cattataagt
    16561 ggacaattaa tttttaattt atggagatga tatttaacaa ttagaagtaa tatataatat
    16621 gcgctaagaa gcaatttttc aactatttat gaaggtggtg gtacaagaaa agtatcacct
    16681 tttttttgtt atacaaaaaa acagttgact ttttatatga accccttcta taatgttact
    16741 gaaattaagt aaaatgattg aacaaatgta aagataattg aaaagcttga ttttggagga
    16801 aatcaaatga aaaaaattgg agacttaaga ttaatgatga agtgttgttc actttattac
    16861 gaagataatt tgaatcaaca ggaaatagca aatcaattgg gaatatcaag accaacaata
    16921 tcaagaatat taaaagaagc ttttgaacaa ggaatagtaa aaatacagat agttgatgtt
    16981 ttgaaaaatg attaccaaaa aatagagaga agtttggaga gaaaatataa attaaaagaa
    17041 gtaatagtag tagatgataa acaagatgca ctaacacaaa aacaagaact agcaagagct
    17101 gtatctgaat atttaactag agtagtaaag gaaaatgata taataggtgt ttcaatagga
    17161 acaacactaa aagaaatacc aagatatgtg gaaaaaagta actgtaaaaa tgttacattt
    17221 atacctctat taggagggat tggagacaat gaaatagata ttcatgcaaa tcaaatagca
    17281 gtaagtctag cgagggcttt tggaggtgat tttaaattat tacatgctcc agctgttatg
    17341 tctgacttgt ctacaaaaga gaaactttgc aaagacgaaa aaattaaaga agtactagat
    17401 ttaatagata aaacaactat agcaattgtg ggtataggaa atcctatgag tttaaactca
    17461 acaataatgg cttctggata catgaatgaa gcagatatag aagatttaaa aaaatacaac
    17521 tcaataggtg caatttgttt acaagccttt gataaagaag gaaaaacttc tatacttgaa
    17581 tttaatcaaa gagtattagg agtgaaactg gaagatttaa aaaagataaa aagaacaata
    17641 ggtgtcgctt caggagatga aaaaatagag gcaataaagg catcattaaa agcgaaattt
    17701 ataaatagtt tagcaattaa tcacagttta gcacttaaat tattagaaga ttgtgactaa
    17761 tagaaagata gataaaagtt aacaggaggg aatgtatatg tcaatatcaa aagaaacatt
    17821 gcttgaaatg tataagagaa tgaatcaggc tagaaaattt gaagagaaag ttagttggtt
    17881 ttttgcaaga ggtatggttc atggaacaac tcacttatct gtaggtcaag aagcgtcaag
    17941 tgtggcggct gtaatggctt tagaaaaagg agatttagta tcattaactc atagaggaca
    18001 tagtcaattt ataggaatgg gaatagacct aaataaaatg atggctgaac ttatgggtaa
    18061 agaaactgga ttttgtaagg gaaaaggagg ctcaatgcat atagcagata ttgagtcagg
    18121 taacttagga gcaaatggag ttgtaggtgg aggtcttaca atagctccag gagctgcact
    18181 tacacaacag tataaaaaaa caggcaaaat agttttatgt agttttggtg atggagcatc
    18241 aaatgaaggt acttttcatg aaggaataaa tttatcttca atatggaaat tgccaataat
    18301 attttactgt gagaataatt tatacggtat gtcaacaagc ataaaacgtc atatgaatat
    18361 agaaagtatt gccactagag cagcttctta tggaatagaa ggaatatcaa tagatggata
    18421 taacccaata gaagtatacg aaacagtaca aaaagctgct gagaagtgta gaagaggaga
    18481 aggacctgta ttaattgaaa gtagaactta tagatggtta ggtcattcaa aatcagatgc
    18541 aaatgtatat agaacaaaag aagaaataga atcatggaaa gcaaaagacc ctattgaatt
    18601 tttaaaaaat tatcttatag aaaataatct atctaatgaa gatgaattag ataagattca
    18661 agaatttgca aagcaatcaa tagaagatgc agtagagttt gctcaaaata gcccaaatcc
    18721 aaaaatagag tctttattag aggatgtata tgcagactaa gaatggtaaa ggaggaaaat
    18781 aaaaatgagt acaagggaat taacatatgc acaagcaata aaggaagcaa tgtcagaaga
    18841 aatgagaaga gatgaaaatg taatatttat gggagaagat ataggaatat atggaggggc
    18901 atttggtgta tcagttggta tgatagatga atttggacca gaaagagtaa gggatactcc
    18961 tatatcagaa gcagctatag caggagcggc agcaggtgca gcagcaactg gtttaagacc
    19021 tattatggaa gtaatgttta tggactttgt aacaatttct atggatgcaa ttgtaaatca
    19081 agcggcaaaa atgagatata tgtttggagg aaaagcacaa gttccaatgg ttgttagatg
    19141 tccaggtggt tctggaactg gttcagcaga gcaacattca caaagtttag aagcttggtt
    19201 ttgtcatgta ccaggagtaa aagtagttgc accttctaca ccagcagatg ctaagggatt
    19261 gttgaaagca gctataagag ataataatcc agtcatattt gttgagaata aattgttata
    19321 cagaaaaaaa ggttttgtgc cagaagatga ttatgtaatt gaaattggaa aggcagatat
    19381 taaaagagag ggtactgatg ttacagtaat tacttatgga agaatgttac aaagtgttga
    19441 agaagcagca gagactctat caaaagaaaa tataaatgtt gaaattatag atttaagaac
    19501 tttatatcca ttagataaag agactatagt aaagagtgta tgtaaaactg ggagagtttt
    19561 aatttgccat gaagcagcta agactggtgg gctaggtgga gaaatatcag ccctgataac
    19621 agaaagtgaa tcatttgatt atttagatgc tccagtaaaa agaatttgtg gaaaagatgt
    19681 gccaatacca tataatccag agttagaaaa agcagttgtc ccaagagttg atgaaataga
    19741 agaagcaata aaatctttaa ttgttagata aggagggata gatatggtgg caaataaaat
    19801 aaaagccaca ccagcagcta gaagtcaggc aagaaaagac aatataaaat tagataggct
    19861 gattggaagt ggagaaaatg gaaggattca tttagtagat gtgttaaatt atttaaaaga
    19921 taataaagca aatactactc ctttagctag aagaatagca gaagatttaa atatagattt
    19981 agaaactatt gtaggtactg gatatagtgg aaagataaga aagtgtgatg tggaaaaatt
    20041 aactgcaaaa gaaactattg tatcaaataa tacatctaaa tatagtgaga aaaaagagct
    20101 gaaaattgaa aatgaaaatt caagcatgtt taacacagta gaaggaatat ttgaaaagcc
    20161 aaatccaatg agagctactg tagcaaaacg aatgtcagaa agttatttta gtgctcctgt
    20221 atttacattc aatatagaag tagatgctac agaattgaag gtacttagag caaagttaat
    20281 cgacacagta aaagaatcaa ctggagtaaa attaactatg acagatttaa tagtaatggc
    20341 agtatctaaa atacttccaa atcatcaagc cttaaattca gcatggacag atgagggtat
    20401 atttagatat aaagatgtaa atatagctat tgcagttggt ttagatgaag gcttatatgt
    20461 tccggtagta aaaaatgcaa acaaaaagtc tttaaaagaa atagcaaaag aaagcaaaga
    20521 acttgcggaa aaagtaaaga cagggaaact tatgccagca gaccaagaag gtaatacatt
    20581 tacaataagt aatgttggaa tgtatggtat aactacattt acacctataa taaatatgcc
    20641 ttcaagtgca atcttagggg taggtgctac acaagataag tttgttccag taaatggaga
    20701 ggctaaaata aaacctataa tgaacttatc attaacatca gaccatagag taatagatgg
    20761 aacagtagct gcaaaattct taaaagattt aaaagaattg ttagaaaatc ctttatcaat
    20821 gcttgtatag gttttgatat ttcgttactg atataaatag tttgaataga ttatattatt
    20881 ataattgttt cagtaaattt aaagctttta tatattaaga attattttta acagtaacta
    20941 tatgtgaata tgatttgctt aattaaaata tatgaaaata aataggagga gagcctatgt
    21001 cagtagaagt aataatgcca aaagcaggag tagccatgga agaaggtact atagtatctt
    21061 ggctaaagca agaaggcgaa gaagtaaaga ttggagaacc tatactagaa ataacaacag
    21121 ataaagttaa tatggagata gagtcagaag gagaaggtac acttgctgta atcatacata
    21181 aagaggaagg tgaagtatta ccagtattta cagtaatagg tgtaatagca gaaaaagggg
    21241 aaaatcaaga ggaagtaaaa gctaaatatt tatctggaaa tgtttcaaaa gaagatatag
    21301 ttgaagagaa tcaaaatata gaagttaaag aagaaaaaat aaacaaaaaa gagtgtaatc
    21361 atgattatga tgtagtggta ataggaggag gacctggggg atatttatca gcattaaaag
    21421 cagctctttt aggtggaaga gttgctttag ttgaagaaaa tatactagga ggtacttgct
    21481 taaatagagg ttgtatccct acaaagactt atataaaaac agcagaaata ttagaagaaa
    21541 ttgaccaatt atcaaagaga ggtgtaaaag taactgtaga taaagaacag gacattaaga
    21601 aggccataaa atataaaaat agagtagtta aaaaacttac agctggtgtt ggcggattat
    21661 taaaaagtag agatgtagat gtgtttaatc ttaaagctag tgttaaagaa gagcataaag
    21721 taattttatc agatggaaaa gttttagata cagaaaatat aataattgct acaggttcca
    21781 aagtaaggat attacctata aaaggaatag aatcaaattt aataataact agtacagaag
    21841 cattagattt ggaaacagtg ccagaagaat tagtaataat tggtggtgga gtgattggtt
    21901 gtgaatttgc agagatattc aattcaagag gttctaaagt aactatagta gaaatggaag
    21961 atagagtaat cccaagaatg gataaagagt taagcgaatc tttaaaatat tctcttagta
    22021 aaaaaggtat aaatgtatta actaaaaaga aagtttctga atttaaagaa gaaggaaata
    22081 acatattagt gtgcattgaa ggtgaagtgc caataaaagc agatttatgt ctatatgcta
    22141 taggaagaga agctaatctt tctggcatag aggacttaga tattaaaata gataaaggtt
    22201 caatagttgt aaatagtaaa atggaaacaa gtataccaag catatatgca gtaggtgatg
    22261 ttactggagg tgtaatgtta gctcatgctg cttttaaaat gggagaggta gcagcttcaa
    22321 atgctctagg tgtgaataaa gaagttgact taggtgcatt acctagttgt gtttatacaa
    22381 ttccagaagt agcatcagtt ggtattacag aagaggatgc tagaaaaaaa tataatgtaa
    22441 aagttggtaa gtttaatttt gctggaaatg gaagagcttt agcttctggt caagaacaag
    22501 gatatgtgaa agtagtagca gatgctaaat atggagaaat tttaggtata catatgtttg
    22561 gatgtggtgt agcagaactt ataaatcatg cagcatcatt taaagcatta gagataccta
    22621 ctgatgaagc aagtgagttg atatttggtc atccatgtac ttcagaagct ttaatggaag
    22681 ctttggcaga tgtaaatgga gaatgtttac acttacctaa aaaataaatc taaacataaa
    22741 ttattaattt aagtctatta ttaatattaa tttatatcat aaaattttaa aacacttact
    22801 tagccatacc ccttattatg caaataattt atttgtataa taaggggtat tttttatgga
    22861 aaaatataga atatagaatc cttgtagttt ttgttgtaca aaaaagatag taatataaaa
    22921 taacattatc attttagaat gatttcaatt gtaattagga aaagtgattt ttttgttttt
    22981 aaaaaaagta ttatttgtaa aatataaaaa aaaatttctt aatatttgaa taaaagaata
    23041 gcttaattta aatgttatta ctttataaaa aaataataag tacaaaaaaa acaatatatg
    23101 gcatataagt tgctatataa ataataagga gggaacaatt ataaatgagg caaagaataa
    23161 aaaacttaat tgaaaatgaa gataagaaaa atcctttaac agatgaagcg atttcaagta
    23221 ggttaaatat tcgaagagaa gatgttacct ttttgagaaa tgagttaaaa atagaagatt
    23281 caagacaaag aagaaaagtt gttttaatta aagctataaa agatatttta aaagaagaaa
    23341 aaactataaa taaaaatgat ataacaagaa gattaaacag cattgggttt acagtatcaa
    23401 gatttacagt catacaatac ttaaaagaga ttcaagattc tggtgaccta aaacttgata
    23461 aagagagtga tgataaaaaa gaagaaataa tcaataatag agaaaataaa gatatagcat
    23521 ttgataaact cataggtagt agtggtagct taagtacaat tataaaacta gccaaagcag
    23581 caattcttta tccacctcat ggtcttcata ctattattat ggggccaact ggtgttggaa
    23641 agagtgagtt agcagagtgt atgtataaat ttgcagtaga atcatccaga ttccctaaga
    23701 atgcaccatt tatagttttt aatgcagcag attattcaga gaatccacag cttttgctat
    23761 cacagttatt tggacacgtt aaaggtgctt ttacaggagc tgatgagaac aaagaaggat
    23821 tagtatcaaa ggcagatgga ggaatattgt ttatagatga aatacataga ttacctcatg
    23881 aaggtcaaga aatactattt caactgattg ataaaggtat ggtcagaaag ttaggagaaa
    23941 caagactaac acataaaata gatgtaatga ttatttcagc aacttctgag ccaatagatt
    24001 ctcatttact taatacattt aaaagacgta tacctgtaac aattgaaata ccagaattag
    24061 ttgcaagacc attaaatgag aggtttgaca ttataaataa tttcttctta atagaagctc
    24121 aaagaatggg agcaaacata catataaagt cagatgtatt aaaagctctt atgctttacg
    24181 attgtatagg taatgtaggt caacttagaa gtgatattca agtagcttgt gctaggggac
    24241 tattaaacca acttacaaac aaactaaaag aagtaaatat aagcatatca gatgtacctg
    24301 gatatgtaaa acaaggtctt ataaaaatca gaaattgtag aggtaaaata gaaaattatg
    24361 tagatgggga tttaattata gattcaaatc ttcaaggtga gatagaaaag aaaaatgaag
    24421 atatatatac ttttccagat gaaatataca aacttactga aagaagacat attgatttat
    24481 tggaacaagg tttagatttt gatgttataa atagaatcat tggaggagaa ttagaaagtt
    24541 gcattcaaaa atacataaaa caagttaaaa aagtttctat tagtaacaat cttccagata
    24601 taagtccaat tgttggaaaa tatatcgtag agctaactta tgaagtaatc aaaatagcat
    24661 cacaatacct aagttcacta gaaccgaatc tacatttatg tttagcagtt cacctaaaag
    24721 cagcttttga gagattaaaa gaaggaaaag taatatcaaa tgctcatttg aaagaaataa
    24781 taggaaatta tccactagaa tattctgtag cagaaaaggt agctcaatat ttaaaggaaa
    24841 gatataatat aaacttacca aaagaagaaa taggtataat agcaatatat tttaaaatga
    24901 gtagtagaag agatatgtct gaacaaaaaa agataggtgt acttcttatg gctcacggaa
    24961 gagtagcatc ttctgtatgt gaggttacaa ataaactatt aggaattaat cacgcaaggt
    25021 atttagatat gccatttgag aagaagccgg aagaaatgat tgaagaagct actacaataa
    25081 taaaagatat tgatgaaggt agaggtgtga ttatattagt agatatgggt tcactttcat
    25141 attttggaga tattatttct ggaaagactg gaattaatat aaggacaata tctagatttg
    25201 atacattgct ttctatagag gttatacgaa gagcagtttt accagattta acaatagacg
    25261 agatagttga tgaagttaaa ggtatagagc cacaagtaaa agaaaaggtt agtaaatgta
    25321 acaatacaaa agtagatttt agaaaaccta taattttaac attatgtatt actggattag
    25381 ggggagcttg taagataaaa aaaatcctag aggaaaatat taaaagtata actgaaaagg
    25441 ttgatataga acctataggt atgattgaat atccaagtct tgatttagag ataaaacaaa
    25501 taagcaatga taaaaatgta atcgccatag ttggaacaat tgaccctcag atagcaaata
    25561 tccctttttt tagtctagta gacataaatg ataaagcaaa gttaaatcaa ataaataacc
    25621 taattgatac aaatcaaatt ggtgaaacaa ataaaaaata tgaattatca gatttaatac
    25681 ataaagaaga tatatttgtg gatttacagg ttaaatcaaa agaagaagtt ctaaaatata
    25741 tgagtaaaaa acttagagag aaaggttatg tgacaaaaaa tttctataaa aaagtagtgg
    25801 aaagagataa cttatttcca actgaattat cagatgaaat agcaatacct cacacagatg
    25861 gaatagatgt aattaggcca gctataagta tagccatctt aaagaagcct attgtgtgga
    25921 gtaaaagtaa agtaaaaata atacttttat tagctgtaga ccaaaagtgt tttaatccat
    25981 taagcactct actctcattt atagaaacag atgaatttaa gaagataaaa aacattaaag
    26041 atagagattt tattagggag atgatattag atggagtcaa tgaaaataat tagtagcaat
    26101 ttaattttta aaaatattga agtgtctaac aatgaagatg cattaaagtt tttgggacaa
    26161 cgtttgtttg acgaacagta tgtaaaagaa agctacatac aagctgttgt agcaagagaa
    26221 aaaaagtatg ctacaggatt accaacagaa atatacggag tggcaatacc acatacagat
    26281 atagtacatg taaatgagcc aggaatagca attggaatac tgaataaacc agtaaaattt
    26341 ataatgatgg gtacagacga tactgaaatt gatgtaaaag ttgtatttat gttagcagtg
    26401 aaagagccac aagaacaatt gcaactatta gaaaaactta tgactatatt tcaagataag
    26461 aatatactaa ataatattgt tgacttaagt gaagaaagtg tgagtgattt attaaactct
    26521 aaattgaaaa attaaacatg taaagtatat taaaaatgta aaattaaaca tgcaaagtat
    26581 gttaaaaaat ataaaaagat tattaaagat ttctaactat agatatcttt aaaataaaat
    26641 aatttcaatg ggggtgacta atgtgactaa aaagatatta gttgcatgtg gaacaggagt
    26701 atgtacttca acaattgcta taaacaaatt aaagaaagca cttcaagaca ttggtaaatt
    26761 ggatatggtt aatattacac aatgtaaagt agtagaagtt gcatcaaaag cacctgatta
    26821 tgatttaata atttgtacta cacaagtatc tagtagtata aaaacaccag tgataaatgg
    26881 attacctttt ttaactggtg tcggaatgga taaattaatc aatgatgtat tagaagagtt
    26941 agaattataa ttaaaaaaat taaggaacat ggtctctaaa atcataagtt ttagagactt
    27001 aaaattaaat aatggagggt ctttatatgg agatattaaa tttcattgtt ggtctaggtg
    27061 cacaagtaat gatgccaata ataatatgta tttttggttt gatattaggt acaaaactag
    27121 gaaaatcttt aagagctggt ttaactgtgg gagttgggtt tataggtctt aacactatta
    27181 tagctttgct aacagataat ttaggtccag ctacacaaca aatggttaag aatttagggc
    27241 taagtttatc aataatagat gtaggatggc cagcagcatc agctatagct tttgcatcaa
    27301 cagtaggggc tttaattatt ccaataggtc ttgtggtaaa tatagttatg ctaattacta
    27361 atactactca aactgttgat gtagacatat gggattactg gcattttgct tttacaggag
    27421 ctttagtggc aggggctact caaagtgtaa tgtggggagt atttgcagca gtagcaaata
    27481 tggtaattgt attggtaatg gcagatttaa ctgctccagg tatagaggaa tatttaggta
    27541 tgccaggtat atctcttcct catggattta cacaggcatt tgttccaatt gctatagtag
    27601 taaataaatt attagaccta ataccaggta taaataagat tgagataaat gctgatacat
    27661 tgcagaaaaa atttggatta tttggagaac ctttaataat ggggtctgta ataggtgtaa
    27721 taattggtat tgctgcgaaa tatgatataa aaggaatatt acaattaggt gtaactatgg
    27781 gagctgtatt aatattaatt ccaaagatgg cagcattatt aatggaagga ttattaccag
    27841 tatcagaagc tgcacaggaa tttattgaga agagatttaa aaacagaggc aagatttata
    27901 taggcttaga ttcagcagta ggaataggtc atcctgttac attatcagtt gcactagtgt
    27961 tggttccttt gactatactt atagcagcta tattgccagg aaataaagtt ttaccatttg
    28021 cagatttagc agtaattcca tttgcacttg tattaatcgt accaattaca aagggaaatg
    28081 tgtttagaac attaataata ggaataatta taattacttc tggattatta atcgcaacta
    28141 acttagctcc attatttacc caaatggctt taaatgctag ctttaaaatg cctgaaggag
    28201 caactatgat ttcaagtatt tgtgatggtg cgaatccatt aagttgggta tttgtaaaag
    28261 ttatgaacta taaagttatt ggaggagtag tttttggagt tatagcttta ggaatggcta
    28321 tttacaatag gaatagaata ataaatgaaa ataaaaagct atctatagaa gaataaggtt
    28381 aaaacaattt tattaaggag tgtataggtt atgaaaaaat ataaatatga ttctaaaagg
    28441 tacttactca gtgttacaat accaggattt tttatgattt taatattgat ttatgcactg
    28501 tttaataact atataaattt taatttgaat atatatagtt tgttaataat tgtctgtact
    28561 tatggtatat tcaatacttt tatatcatca agtaatccta aaaaaattat tattgacagc
    28621 aaatgtatac attttacctc atttatgact acacataagt atgaaattaa agatatagaa
    28681 aagataagaa taagagaatt ttataataaa aaaatttatc taaaaattaa tgatggagac
    28741 ctttttaaag gtaggtattg gataagaaca aatatgttta ataatagtat agcattatat
    28801 agttatttca ttgagttgga agagtgctta tatccagatt cactaagatt tagaaataaa
    28861 aaatttgaga ataagaatat ataaaaataa aaaattgaat tgaagcctaa cttaatcttt
    28921 tttgataaga tataagtttg aactttatac taaattttta gaattttcaa tcaagacaat
    28981 aacataatag ctaagcaatt agaacacaac taagatgtat aaggcatgta tatgatgaat
    29041 gggaggattt aattaatgtt attagagaaa ttaagaaaag aagtattaca agcatcttta
    29101 gaccttttaa actataattt agtgacatta acaggaggga atgtaagtgg gagagatgaa
    29161 caaactggct atatagcaat caccccaagt ggaatggatt atagaaatct tacaccaagt
    29221 gatattgtta tagtagatgt agatggaaat attatagatg gaaaatggaa agcatcagtg
    29281 gatttatcag accacttgta tatatataag catagagaag acataaatag tataattcat
    29341 acacattcta catactcaag ttgttttgct attttaaatg aacctataga atgcgcaagt
    29401 acaactcttg caaatgaagt agggggttca gtaccagttg caaaattttc accaccaaca
    29461 tttaaaaaga tgggaaaatg tgttattgaa gcaattggtg acaaaagagc atgtctttta
    29521 gcaaatcatg gcgtaattgc agttggtcca tctgtaggac atgctcttac agccgctgta
    29581 atgttagaag attcagcaaa agtatattac ttagcaaaat caattggcac accagtgcta
    29641 ctgcctgatg aagaaattca aagagcgaga gatgtatttt ttaatgtcta tggtcaggat
    29701 aaataaatta gataaaattt taaaagtaat atgaaaatat agatattcaa tgatattaag
    29761 gagatttata atgatataat aagtttaata gttaagaagt aaaatatgaa atgtaatagt
    29821 atataaattc atttaagact aaaaataata agataaaata aaaaggagtc tgtatttcta
    29881 tgtattactt attaaacaaa agcacaaatc cttactttaa tttggcatta gaagaatatc
    29941 tatttttaaa tgataaatat aatgatgata ttataatcat atggagaaat gaagactcca
    30001 tttttatagg taaaaatcaa aatccatatc aggaagtaca tcatgatgtt attgaaaagg
    30061 gagaaattcc tattttaaga agaatatctg gagggggcac tgtttatcat gatttaggta
    30121 atatcaatat gtcattcata caaaaagata ggcaacttca tgagatagat tttttggagc
    30181 atactaagtt tatgcaaaag atgctttcaa ccttaggact agatgtatct ataactgaaa
    30241 gaaaagattt atttttaaat gggaaaaaaa tttcaggctc tgcacagtct ataaaaagaa
    30301 aaaattctct ttatcatgga actcttcttt acgattcaga tttaaataaa ttaactaaat
    30361 atttaaattc aaataaagca actgaatcaa atgcaactaa atctgtttca agtaaagtta
    30421 caaatattaa gtttttactc gaaaaagata taaattactt tttagattat tgtgttgaat
    30481 atttaaaatc taatataaag aatataaaag aacttgaatt ggatgaagaa gatattaaaa
    30541 gtgtatacaa tataggagaa gataaatata gaaagataga gtggacatat ggtaaaacgc
    30601 cgaaatttca agtagtgcta ccatttaata atgaaagtaa aataaatata catgttaaca
    30661 gatggaaagt atcaaaattt tttatttctt atgatgataa tgtaattaat atggataagt
    30721 tgttagattt gaatttcttt aaagaagaaa taaaagagag tattttagaa a
//
LOCUS       DACTGW010000020        30710 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.020, whole genome shotgun sequence.
ACCESSION   DACTGW010000020 DACTGW010000000
VERSION     DACTGW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.020"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            165..2066
                     /locus_tag="I9573_000732"
     CDS             165..2066
                     /locus_tag="I9573_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT4795366.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASNLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2069..3112
                     /gene="ypdE"
                     /locus_tag="I9573_000733"
     CDS             2069..3112
                     /gene="ypdE"
                     /locus_tag="I9573_000733"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAT4795367.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3127..3432
                     /locus_tag="I9573_000734"
     CDS             3127..3432
                     /locus_tag="I9573_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAT4795368.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPTMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3529..3864
                     /locus_tag="I9573_000735"
     CDS             3529..3864
                     /locus_tag="I9573_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4795369.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3896..5218
                     /locus_tag="I9573_000736"
     CDS             3896..5218
                     /locus_tag="I9573_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010492767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT4795370.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            5228..6298
                     /locus_tag="I9573_000737"
     CDS             5228..6298
                     /locus_tag="I9573_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAT4795371.1"
                     /translation="MNNTIERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6303..7490
                     /locus_tag="I9573_000738"
     CDS             6303..7490
                     /locus_tag="I9573_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAT4795372.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVANNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7687..8559)
                     /locus_tag="I9573_000739"
     CDS             complement(7687..8559)
                     /locus_tag="I9573_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAT4795373.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVSYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYKQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLHLNPEVFKNDALIYTNIDEVSIYNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8953..9144)
                     /locus_tag="I9573_000740"
     CDS             complement(8953..9144)
                     /locus_tag="I9573_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795374.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9364..10410
                     /gene="cobT"
                     /locus_tag="I9573_000741"
     CDS             9364..10410
                     /gene="cobT"
                     /locus_tag="I9573_000741"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAT4795375.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10442..11002
                     /gene="cobU"
                     /locus_tag="I9573_000742"
     CDS             10442..11002
                     /gene="cobU"
                     /locus_tag="I9573_000742"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAT4795376.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            11012..11785
                     /gene="cobS"
                     /locus_tag="I9573_000743"
     CDS             11012..11785
                     /gene="cobS"
                     /locus_tag="I9573_000743"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAT4795377.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWIIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11805..12419
                     /locus_tag="I9573_000744"
     CDS             11805..12419
                     /locus_tag="I9573_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAT4795378.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12486..12665
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12780..14294
                     /locus_tag="I9573_000745"
     CDS             12780..14294
                     /locus_tag="I9573_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HAT4795379.1"
                     /translation="MGKKIMLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMAL
                     NSFITKEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSS
                     KYHDYKLELVGVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPV
                     IIVGDIDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKV
                     PVIGVVPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVS
                     IRYVDYGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQ
                     MLGKKLKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGK
                     KVKGYEIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEIS
                     FTRAILNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14333..15709
                     /locus_tag="I9573_000746"
     CDS             14333..15709
                     /locus_tag="I9573_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HAT4795380.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15710..16681
                     /locus_tag="I9573_000747"
     CDS             15710..16681
                     /locus_tag="I9573_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAT4795381.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYVSSFVSMLVFTLIHVLCRMILINLII"
     gene            16713..17783
                     /locus_tag="I9573_000748"
     CDS             16713..17783
                     /locus_tag="I9573_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAT4795382.1"
                     /translation="MKDLGHGANVGEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIGINPDFIIPGNGATEVIYLLMKSVKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLGKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRAYKKTTKELKKDLLKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17785..18672
                     /locus_tag="I9573_000749"
     CDS             17785..18672
                     /locus_tag="I9573_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAT4795383.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSEVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVIILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDTLCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18660..19292
                     /locus_tag="I9573_000750"
     CDS             18660..19292
                     /locus_tag="I9573_000750"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HAT4795384.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19301..20527
                     /locus_tag="I9573_000751"
     CDS             19301..20527
                     /locus_tag="I9573_000751"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HAT4795385.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCKERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20524..21132
                     /locus_tag="I9573_000752"
     CDS             20524..21132
                     /locus_tag="I9573_000752"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HAT4795386.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNVVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKIPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            21122..21697
                     /locus_tag="I9573_000753"
     CDS             21122..21697
                     /locus_tag="I9573_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HAT4795387.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21690..22442
                     /locus_tag="I9573_000754"
     CDS             21690..22442
                     /locus_tag="I9573_000754"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HAT4795388.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22426..23547
                     /gene="cbiG"
                     /locus_tag="I9573_000755"
     CDS             22426..23547
                     /gene="cbiG"
                     /locus_tag="I9573_000755"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HAT4795389.1"
                     /translation="MNIEGNIAIICITENGKNLAVKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIIFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPIAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23544..24269
                     /gene="cobJ"
                     /locus_tag="I9573_000756"
     CDS             23544..24269
                     /gene="cobJ"
                     /locus_tag="I9573_000756"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HAT4795390.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24266..25018
                     /gene="cobK"
                     /locus_tag="I9573_000757"
     CDS             24266..25018
                     /gene="cobK"
                     /locus_tag="I9573_000757"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HAT4795391.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            25037..26515
                     /locus_tag="I9573_000758"
     CDS             25037..26515
                     /locus_tag="I9573_000758"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HAT4795392.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEEDGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26545..27450
                     /gene="hemC"
                     /locus_tag="I9573_000759"
     CDS             26545..27450
                     /gene="hemC"
                     /locus_tag="I9573_000759"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAT4795393.1"
                     /translation="MNIVVGTRGSTLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27440..28939
                     /gene="cobA"
                     /locus_tag="I9573_000760"
     CDS             27440..28939
                     /gene="cobA"
                     /locus_tag="I9573_000760"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAT4795394.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEDIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDNDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEDKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28956..29921
                     /gene="hemB"
                     /locus_tag="I9573_000761"
     CDS             28956..29921
                     /gene="hemB"
                     /locus_tag="I9573_000761"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAT4795395.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     NSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30074..30652
                     /locus_tag="I9573_000762"
     CDS             30074..30652
                     /locus_tag="I9573_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HAT4795396.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12432 a         3065 c         5671 g         9542 t
ORIGIN      
        1 attgaactat atattttatt attataaaat atgtatatta aattttggaa tcaaaatata
       61 ctaggatata aaattcttaa ataaaaagaa aaggttgttt ttataaaaaa tattttttat
      121 accactttaa aaaacacaat aataacactg agggaggtgg ggatatgaga gaacgcatta
      181 ttcttgaaac attaaaaaat agtagtggtt atctaaacat agattatttt gcagataagt
      241 tgggggtatc tactcgaact atcagaaatg aaataaaaaa gatagaaact ataaaagaat
      301 gtaatggttt taaattagaa tataagtcta aattaggata tattctaaat attaaagacc
      361 aagataaatt cgagcattat ctaagaaact taccctcaga tttagttgaa aatccagaac
      421 aacggttgga gtctataata gtagagcttt tagtaaatga aggttataaa actatagaac
      481 agttaagtaa aaaatttcta gtaagttcat cccaaataaa aaatgattta aaaaagatag
      541 atgaaaagat aaaggataca gaattaaaac tagagcgaaa ggctcattat ggaattaaaa
      601 tagaaggttc tattaaagaa attcaaagta tattggtgga tagttatttt agagggaata
      661 gaaatataac agagtacagg aataagttta tagataattt aaaattagct aatattagaa
      721 gtacaataaa aaatgtactt aatgaacatg atttagaagc aaatttaaca gaacttgagg
      781 aaatattagc acaaattatt atattgtata taagagttag tatgagagta gtgggaaatg
      841 ctagcaattt aaaattatat acagaagatt taattataga taaattatta gataaaattt
      901 ttagagataa aaaatataac cttaattatg atgaaaagta ttatttaaag gagcttataa
      961 aactaaagac taaagataag aaagccacta taaagaatat tgataagaat aaacttcaag
     1021 atattatgtt cgaatttttt agagagatag ataaaaaata taactctaat tttttagaag
     1081 ataaagagtt ttttaactta ttttacttac atattgcatg tttaatagaa cgtataaaga
     1141 agaaccacaa gataataaat cctttttcag ttaaaataag tcaacagtat ccaactgttt
     1201 ttaatttatc tattcaattt tcaaaaatca tagaaaatga gtatcatata aaaataagtc
     1261 aagatgaaat agggtttata gctactcaca ttgctgttcc ttttgaaaag agagaagaag
     1321 ctaattttaa taaaaaatat aaaatagcaa taatatgctc ttctggtgga ggtagtgcat
     1381 ttttgataaa tttaagactt aaagaaattt ttccaaatgc tgagataaga aatttttcat
     1441 tactagaaga aaaagctgta atagaatttg caccagattt gattttttca ataacaaatc
     1501 ttttatttga aataaatgct ccagttatac ttataaatga aattttagac gaattggatt
     1561 atttagatat taaagaaagt gttagatttg cagaccatat aggaaatata tcaagcccaa
     1621 aacaatatat attaggtcta tttgataaga atcactttag gtgtataaaa gataaagcta
     1681 agtataagga tatccttgat agtatgtcaa aatgtatagt tgacgaggga gcttgttctc
     1741 caacctatcc caaagatgta tgggaaagag aaagctattt gagtactatt tatacaaatg
     1801 gggtggctat acctcatcca atcgagatga caggaaacaa aaatataata tcagtagctt
     1861 tgatacaaac tgatattatt tatgaaaata gggtgcccaa aattattttc atgatttcat
     1921 taataaaggg aaatttagaa cttcataagc aaatatctaa atatttaagt aagattatgg
     1981 caaataagga tatggtagac atgctaaata agtcacaatc ttatgaagag tttatgtata
     2041 agttaaaaat ctatctggga ggttagacat gaatatagag ttttttaaga ggttgtcaga
     2101 agcagatggt atagcatcta atgaagaaga ggttagacat gttttattgg aagaactaaa
     2161 ggcttatagt gataaaatta tctgtgatgg gctagggagt atcatctttt ctaaaataaa
     2221 agatgagagt gcaccaaacg taatgatttg tgctcatatg gatgaagttg gatttatggt
     2281 gagaagtata gacaaacttg gtatgattca cttaataaca ataggaggag taaaacctct
     2341 tgcacagttt gtacaaaaag ttagaattac aacaaaagaa ggaaagaaaa tccctgctgt
     2401 cataaatgct acatataata atggtaaggc tgaaaatata tatgcagata ttggagcata
     2461 tacagaagaa gatgtatata acctagggat taatgttggg gatatggtaa catatacaac
     2521 aagttttgag gaatttactt taccagaccg attagtggga aaggcttttg atgataggat
     2581 aggatgtttt gtgatgggag aagttttaaa agaacttagg aaagaaaatc taaattgtaa
     2641 tattcacttt gcggctacaa gtagtgaaga ggttggaata aggggagcaa agacatcaac
     2701 acagttaata aatccagata tagtatttgt aatagatgta gcatgtgcta aaaatgagtt
     2761 tgtgagagac tatacaaatc agaaacaaat tgataaaggt gtaatgctaa tgcatagaga
     2821 taggactcta gtccctagta gaaaaatgat agattatgta atggaaattg ctgataaaaa
     2881 taatatacca cttcaacatg atatgtttga atcaggtggg actgatggag gagaagcaca
     2941 tctcgtaaat gaagggaaac cttgtgtagt aacttgtgta ccagttagat atggtcattg
     3001 tgcattttca atagtaagta ataaagattt agagaacatt ataagattgt atacacagtt
     3061 aattttaaat tttgatgaag aacaatataa acatataaga aattttatct aaaggagaat
     3121 aagaaaatgt caacagaagt acaaataatg gagcttatat caactgcagg agagagtaaa
     3181 tcgaaagctt ttgaggcttt aaaaaaagta aagtcaaaag attttaaagg agcaaaattg
     3241 ttaatagagg aggctcgtaa aatagatatt gaggctcaca gaatacagac agaattaata
     3301 actcaagatt taaatgaaga tgttgaaagc ccaacaatga gtcttttaat ggtacatgct
     3361 caagaccact atatgacttc tcagctagca aaagacttga tagaggcttt gatatattgt
     3421 ctcgataagt aattgtaatg tagaaataga ttatttatgt aaaaattctt ttaaaagttt
     3481 agatgctttt aagagataat aaaaatacaa actttaggag gaattattat gaaaatatta
     3541 ttatgttgtg caggtggatt ttctactaat atgttaatgc aaaatatgaa aaaggtggta
     3601 aaagagagtg ctaaactcaa tgaaaaagat tttgagttta cagctatacc agcagattca
     3661 atagaaagtg aagttgatga ttgggatata gtacttatag gtcctcaaat agctcataaa
     3721 atagacttct taaaaggaat attagagcca aaaaataagc cttttgcagt aatagataaa
     3781 gatgtatatg gaaaaatgga tggagctaca gttatgaagt tggcattaat aacttataag
     3841 aaacatcaat tagctcaagg ataataaaaa taatgaggga aaagggagat tagatatgtt
     3901 ttcaaaattt gaagcattta tgaatagagt atttatgcct ttagctcata aggttgacaa
     3961 tcaacgtcac cttggttcta taaaggctgg tatggttgct atgacaccat tcacaatatt
     4021 aggaagtata tttgcaatac ttccagcact tccaaatatg cttggagaaa ataatccaat
     4081 aagcaaattc atacttaaca attcagcaat attggattta ccagtaagtc tttccatagg
     4141 tttaatagga ttatatgctt gtatgtgtat tgcttttaat ctggggaact actataaatt
     4201 atatatacca ggttgtgttg cactatcaac tttttctttt ttattcctag tagcctcatt
     4261 tacaccagat ggagctttag aaataggaaa tttaggagct aaagggctat ttacaggtat
     4321 gatagcaggg cttggttctg tggagttata taatttttgt aataagaaga atatcacaat
     4381 aaaaatgcca gaaaatgtac ctgattttgt atctaggtct tttgaattaa tacctactac
     4441 tataatagtt tgtggaactt ttatagttgc tcgttttatt tcattaaaag tatttggaga
     4501 attacctcca cagatactta ctcaattttt atctccatta gtaggaagta tggataaccc
     4561 atggtcagtt cttgcactta actttgctat atgtactata ttcttttttg gaatacattc
     4621 atcagtattt agtcctataa ctagacctat aatgattaca tttatagctg aaaacatagc
     4681 agctatgcaa gctggagaaa caatacctca tttctttaca gcaggagctt caagtgcatt
     4741 ctttggattt acaggatgtg gaattagtat aggttgtgta attgcatgta tgttatcaaa
     4801 aaatgcttcc tataagaaga ttggacgtgt atcattattc cctgcattat ttgggataaa
     4861 tgaaccaata ttatttggag caccgataat attaaatcca ataatgttta taccacatgt
     4921 atttggtgga gctataatag gtacactacc aatgtttttt atgcactggg gatggttagc
     4981 aaagccagta tttgaccctc catatgtagg ggtattctta gaaggatttt taactaatgg
     5041 agattggcat tctatattag caaatgcatt gcaattagta ttagcagttt tattatattg
     5101 gccattcttt aaaataatgg aacgtcaaca aaataataaa aaacaaaaag aagaaactaa
     5161 gaatatattc tctaaagaag aagaagactt attggatgat ttaggattgg atttctaata
     5221 ttttgttatg aataatacaa tagagagatt agtatcctta atctataaaa gaggtgttga
     5281 cgctcttttg attaaagcaa aaaatactaa aagatatata ggagctttat caggtagtgg
     5341 tgtatatgtg cttgttacta aggataaaaa ttatcaaatt ctagatggca gatatattga
     5401 tgaagcagat aaaaaaacga ctggtttcat aaaaaggatt gtatctcaag gttcttatat
     5461 accaactata attgagttat taaaagaatt aaatataaaa aagttagcaa tagaaaacca
     5521 agctatgagt atacaagagt atctttcact aaaaaatgaa ggttttgaaa tcaatttaat
     5581 aacaaatgaa ttatggaagc taagggctat aaaatctaaa gaagaaattg aacttattaa
     5641 aaaggcttgt gagattacag atgaagtatt tgatgaggtt atttctgaaa ttaaaaaaga
     5701 tatgactgag ctagaagtat cagctctaat tcagtatcat gcattaaaaa aaggtgcaag
     5761 tggaatgtct tttgaaacaa tagtggtatc aggagaaaga ggtgcaatgc cacatggaag
     5821 acctacaaac aaaaaattga aagtgaatga agctataaca atagattttg gtgttgttta
     5881 tcaagggtat caatcagaca tgactcgtac aatatctata ggtaaacctc ctaaaattat
     5941 taaagagata tatgatgtag tattggaagc acaattatct gcaatagagt caataaagga
     6001 aggaactagg gcaagtgatg ttgacaaagt ggctagaaaa attatagata aacatggatt
     6061 tggcgaatat tttaatcatg gactgggtca tggtataggt cttggagatg gtgaagttcc
     6121 cactttaaat ccaaatagtg aagatatatt agtggaagga atggtaatgt cttgtgagcc
     6181 aggtatttat attccaaatg tagggggagt tagaatagaa gatgatattg ttataataga
     6241 tggaaaaggt gttccactca ataagacatc caaagagttt ataatattag gagagtaaaa
     6301 ttatgaaata taattttaat aaagtattaa atcgtaaaga aggaaattgt agaaaatgga
     6361 gcaaccatgt gattaaagaa aaattcggct taaatgaaga tgcaattcct atggatttag
     6421 ctgatatcga ttttgagtgt gctcctgcta taaaagaatc tatcttaaaa agagcatcag
     6481 taggagatta tagttatact tttataggag atgacttcta tgatgctatt ataaattgga
     6541 ataaaagaag atttgatgta tatatagaaa aggattggat aaaattaact tttggtactg
     6601 tttcaaccct tcattatata gttcaagcat atactaaaga aggagagggt gtattgataa
     6661 atacacctgc ttatgaccct tttgcagagg ctgtagcaaa taataataga aagttgtgtt
     6721 gtagctcatt aaaattagat aacaatagat attatttaga tttagaggat atggaaaatc
     6781 aaatgaaata cgagaatata agattactta tattctgtag tccacaaaat ccatcaggtc
     6841 gtatatggac aaaagaagaa ctatatcaag tgtcagagtt atgtttaaag tataatgtaa
     6901 ttttggtaag tgatgaaatt catagagata taatatttaa agattataga tttgtatctt
     6961 tgtggaatgc tcatccagaa atttataaaa atagtataat ctgcgtatct cctaataaag
     7021 gcttcaactt aggtggatta aaaacatcat atgtattaat aagagatgta aaaattaggg
     7081 agactttatt agaaaggcta aaaagtaatt caataacttc tccaaatgta tttgcaatac
     7141 cagcaatagt tgcagcatac aatgaaagcg aagaatggct agatgctatg actagttatg
     7201 tagaggagaa ttttgaaata gtttatgatt tctttgaaac taatatacca aaagccaaag
     7261 tgatgaagtc agactcttct tttttagcct ggatagatgt aagagaagta tttagaaatg
     7321 aagaagaatc taaagaattt tttagacatg caaacttaac tatggtagtt ggaagttatt
     7381 ttgtgaaaga tggagatgga tttattcgta ttaatgtggg gtgtcctaga gaaacattaa
     7441 atgaggctct aaatagaatc aaaaaaacgt atataagtat gtattgttaa atcataaaaa
     7501 aagtatctat ggtttatata aaaacacatt atttagttat caaatattac tggaattttg
     7561 tattccagta atattttttg attaaggaat attattgagg aatattaaat aatattattt
     7621 cataccctta tattaactta tataaaaata ttttattatt ttgggaacgg gttagctaac
     7681 ctttatctag tgtatattta tattgtaaga agcagtaaaa acttttccct tactaagttt
     7741 attaataaac ttcttatctt tataaatttt gttagaattt atactatcag caagaccata
     7801 ccaaggttct atacataaaa aaggagcagt agaattagtt tcactataat aaggagtcca
     7861 aataccaact agagggaaat cttccataga tatagttaca tatttactac catcattatt
     7921 atatatagac acttcatcta tgttggtgta tatcaaagca tcattcttga atacttctgg
     7981 atttaaatgt aagtttttta attttccaat aggtattata tcatctgtaa aagaaccatt
     8041 taaattgatt ttttctacat catttttact tttaaactca aggtagtgtt tagaaaaatc
     8101 attttgtttg taaaaaggaa ggttgaatgc aggatgtcca cctatagaaa agaatatatc
     8161 tttagagtca gtatttttta caatccattc tacctcaata ctattatcat ttaatgtata
     8221 gtttatgatt agctcaaaag aatatggata cttctttaaa gtggattcat tatctgttaa
     8281 tttataagat acaaatgtat ctcctttatc tacaatctca aagtccatat ctctagcaaa
     8341 cccatgttga gtcatgctat ataaattttc ttctattatt gtttcattat caaaaagttt
     8401 acctactata gggaacaata taggtgattg tctaccccaa tattttttat taccatccca
     8461 aagaatgttt ttgtcgtatt ttttagaaaa tattttagtt agttcagcac caaaactgct
     8521 tgattcgata attaaatttt cattttgtaa tatattcatt taaaatctcc tttattatat
     8581 tttatgagta tattatacta taaagtataa agattattaa attttatttg atattatatt
     8641 tgaaaggtag attttagtat attttgaaaa ttttgttttg aaaatttggg attatttaag
     8701 aaattttgta ttataaatag aaggaaatag ataaattgta tagaataaaa tatattaaga
     8761 taagttaata atgttaaatg ggaagtgagt ttatggaaaa accaaataca aaagttgtta
     8821 actttcaaga gtataaagac agaaaaaacc aaagagaagt aaatagagac gaacttttaa
     8881 agcttataaa aaaaattgtg acttcaaaat agggagttgt taaactccct attttaatgg
     8941 gtttcataat aattaagaca ttctgagtcc atctatataa tctttgaatt gtgagaaatc
     9001 tgattcacta cttaattcta tggtttctga accttccgta taaaaattct taagatagat
     9061 agtataatta ttatcaacac aatcatgata aatacatcta tatccatctt catacaaact
     9121 ttttaactta gaaaaatcat ccacaatatc atctccttaa agttctttta aatatatttt
     9181 tacaaaaagc atctaattgt atgcaaattg acaatgtaaa ataaaggaaa tgaacataaa
     9241 atgtagaata atttttatat gtatgtaaaa ataaaaagaa aaagttgaaa tttttagaaa
     9301 ataagagtat tttttatcta aaaagagagt ttaaaaaata aaaaaatgtt gggaggataa
     9361 aaaatgagtt tattagaaag tatatcaaaa aacatatatt ccctagataa ctcttctatt
     9421 gaaaaaacaa agcagagatt ggataggctt atacatccaa ctggaagctt aggaaagata
     9481 gaagatattt gcatgcaatt ggcaggaata tttggcaatg aaaattttga cacaagtaaa
     9541 aaggttataa ttgcttttgc gggagaccat ggtgtatatg aagaaggcgt agcacctgac
     9601 cctcaaaata taacaaaatt acagtttcca aatttttcga aaggtctatg tggagttgga
     9661 gtaataagta aatttgtagg ggcagatgtt gtagctgtag atgtaggaat aaactgtgat
     9721 gaaaaactgg atggggtatt agactacaag ataagaaaag ggacatcaaa tatggcaaaa
     9781 ggtccagcta tgagtaaaca agaagccatt agatgtttag agataggtat tgaaatagca
     9841 gagcagtgta tagaaaaaga ttataaggtt attggtattg gggagatggg tatagcaaat
     9901 actactccaa gtacagcaat aatatctgta atttcagggt gtgacccgtt agaagttact
     9961 ggtataggtg caggtcttaa gaaagaaaga ctaaagcata aggctgaagt aattagaaag
    10021 gctattgaaa ttaataatcc aaatccaact gatggagtag atattttatc taaagtagga
    10081 ggatttgaaa taggctcaat ggcaggtgtt atattaggtt gtagtgctaa taggattcca
    10141 gtagtaattg atgggtttat ttcttatgca gcagcattaa ttgcatataa gataaatcca
    10201 aaaactagag aatatatgat agcatctcat ttatcagcag aatcaggaac taaaagagca
    10261 ttagacatat taaaattaga ccctctatta aatatggata tgagattagg tgagggaagt
    10321 ggagctgctt tagcatttga tataatagaa gcttcaaact atacttacaa aaatatggca
    10381 acttttgatg aaattgacat gggaagataa atataaaaat ttaatatgta ggagtgtttt
    10441 gatgagtaaa attattctag taacaggagg agctaggtca gggaagagta actttgcaga
    10501 aaaactatgt tttgacagaa ataataatac agcttatata gcaacctcta ttccatttga
    10561 tgatgaaatg aaagatagag taaaaaaaca tcaagagaat agacctagaa actggaatac
    10621 ttatgaaata tatgaagata tatattccat aattaaggag atatataaag aacatgaaac
    10681 tgttatacta gattgtgtaa cattattagt aaataatcta atgtttgaac acaatctaaa
    10741 tatagaagag tctagtcaag aagaaataaa caaattagaa caatacataa aagaacaagt
    10801 tgtcaaactt attgaagaaa tagaaaaaac aaatctatac tttgtatttg taacgaatga
    10861 gttaggtatg ggaattgttc ctgggaataa acttagtaga atttatgctg atattgtagg
    10921 tcgtataaat caatatatag cttcgaaaag taatgaagtt tattttgtag ttagtggcat
    10981 agctatgaag ataaaggagt agtatgcaat tatgaaaaga tttattttaa tactacagtt
    11041 tttgacaagg atacctataa aattaaatgt aggatttgat gatgaatttt ataagtcaat
    11101 agtatatttt ccacttgtag gttttgtcat aggtatatta tcctatttaa taggatggat
    11161 ttctatgctt ttatttgaac catttatagc ttctataata ataactctag ctggtgtctt
    11221 aattactgga ggacttcata tagatggatt aggagataca tttgatgcaa tatatagtta
    11281 tagggataaa gaaaaaatgc ttgaaataat gaaagattca agattaggta caaattcact
    11341 tctagcaatt atgtttgtac ttttattaaa ggttggattc gtttacgata taataagtaa
    11401 taattcattg tggattataa tttttatgcc aatgattgca aggttaggag taatgttatt
    11461 gacatataaa accgtgacac caagagaaaa aggaatggga aatttattta taggaaaatt
    11521 aactacaagt atgttaataa ctgctataat atacacatta ttgatagttg ctcttattac
    11581 taaatttata tttttattac ctaatatagt gttgattaaa gtattaggtt cgattatagt
    11641 ggtttttgta tttataatat tattcaaaaa acatatttat aaaaaaattg acggagtaac
    11701 aggagatata ttaggctgtg gaatagaact ttcagagctt gtatatttaa tttacatata
    11761 tttgttaatt tttatgtttt tttaaagtaa aaagtggtga taatatgata agattaatat
    11821 taataagaca tgcactgaca aatgataata aaaaaggaag attatcaggc catataaata
    11881 gttgtattag tgaagagggc aaactacaaa taaataaaat taccagatat ctgagtaatg
    11941 aaaacataga taaggtttat acaacaccat ctacacgtac taaagataca gtagaaaaaa
    12001 tatcaaaatt gaaattatta gaaatagaag aaaaagaagc tttaagagaa attagttttg
    12061 gagactttga aggaagaact tttgaagaaa ttaaaatcaa atatccaaat gagtttgaaa
    12121 aaatgataaa agaaggtaac aactatagat atccaaatgg agaaagtttg atagactctt
    12181 ataaaagagt ggcaaaagaa attgataata taattttaga aaatgatagt aatttagata
    12241 caaagacaat acttatatgt tctcatgcag gaacaataag aaatataata acacatttga
    12301 tttctggtag ttataagtat cactggaact ttaaaattga taatgcatct attacagttt
    12361 tagaaattga tggtggattt gctgtaatag ataaaatgaa ttttacagac ttcatataat
    12421 ttaatcaaaa ccaaacatta caatctgtgt ttgtaatata tctgtaaaat tagcaatgaa
    12481 aatagaatat tagattaaaa ttaggttctt aattagatgt taaaagggaa aaaggttaga
    12541 tgcctttgca gcccccgcta ctgtgaaacc aacgaaacta taaataccac tgtcagtttg
    12601 atgggaaggt tatagaagta ggatgaagtt aagccaggat acctgcctaa tttaatttaa
    12661 aacaagaggt cttcggggtg aaagattttt aattaatata tataaaagtc ctatctttag
    12721 aagtattagg acttttttta atttgttttt tagtaaattt taacaaggag ggatggaaag
    12781 tggggaaaaa aataatgcta caaggaacag cttcaaatgt aggtaaaagt gtcttagtag
    12841 caggtttgtg tagaatattt aagcaagatg gttatactgt tgctcctttt aagtcacaaa
    12901 atatggcttt aaattctttt ataactaaag aaggattaga aatgggaaga gcacaagtat
    12961 ttcaagcaga ggctgcgaaa atagagccaa tagctgatat gaatccagtt ttattaaaac
    13021 cttctgggaa taataaaagt caagttattg taagaggtaa agttgtagga gaaatgcctt
    13081 cttctaagta tcatgattat aaattggagt tagtaggtgt gttgaaggaa acatttaata
    13141 gcttaaattc aaggtatgat attgtagtta tggaaggggc aggaagtaat gcagagataa
    13201 acttaaaaga tagagatatc tctaatatgg gtatggcaga aatagctgat gcacctgtaa
    13261 taatagtcgg agatattgat agaggaggcg tatttgcctc attagcagga acaatgcttt
    13321 tattaaatga agatgagaaa agaagagtaa aaggtgtaat aatcaataaa ttcagaggaa
    13381 aaaaagagct tttacaagat ggtgtaaaga tgctagaaga catcataaaa gtacctgtta
    13441 taggagtagt accttataca gatataaaaa tagaagatga agacagtgta actacgagat
    13501 ttaagaaaaa aatgaataaa agtgatatac acatagaaat aattagaacc ccacatatgt
    13561 caaattttac agattttaat atatttgaga cacaagaaga tgtaagtatt agatatgtag
    13621 actatggaga atctttagga aatccagata tattaataat acctggaact aaaagtacta
    13681 tagacgattt aacatatata agaaaaagtg gtcttgaatc gcaaatcaaa aatttacata
    13741 gtcaaggtaa attgatattt gggatatgtg gtggatacca aatgttaggt aaaaaattaa
    13801 aagaccctta tcatgttgaa agtgaaattg aagaagttga tggaataggt cttttagata
    13861 cagaaacaac gtttgagaaa gaaaagacta caacacaagt agaggctact attgttgaat
    13921 gtatcagtga atacatgaat aaccttaagg gaaaaaaagt aaaaggttat gaaattcata
    13981 tgggcattac aaagcgaggt aatgatgtaa gaagtttaga tttaataact aaaaaacttg
    14041 ataaagaagt gaattatact gaaggaagtg taaataaaga ggggaatgta attgggactt
    14101 atcttcatgg aatatttgat gaaataagct ttacaagagc aattttaaac aatataagaa
    14161 agaaaaaagg tttaggagaa ttagaaagca aagtaaactc ttttaatgaa tttaaggata
    14221 aagagtatga taagttggct gattttttaa gagaacattt agatatagag aaaatatatg
    14281 agattatgaa ataatgatga cctttattta taatttaggt aggaggtgta ttatgaaaaa
    14341 aattttaatt gctggtacta atagtggagt aggtaaaaca acaatatcat taggaataat
    14401 gcaagctttg gttaagagaa atatgaaagt acagccatat aaagtagggc cagactatat
    14461 agacccttca tatcacactt ttataactgg aagacattct agaaatttag attcttatat
    14521 gttagatgat gaaaaaataa agtatatagt aaataagtcc tcaaaagatg cagatatttc
    14581 agtaatagaa ggtgttatgg gcttatatga tggttttgga atagatttag ataattgtac
    14641 aagttcacat acttcaaagg tattaaagtc acctgttata ttagttataa atggtaaatc
    14701 tatggcagca tctagtgctg ctatggtact tggatataag gaattagata aagatgtaaa
    14761 tataaagggt gttatagtaa ataatgtaaa gactaatagc cattatcaaa taattaagag
    14821 ttctatagaa aaatattgta atgttgaagt attggggtat tttcctccaa ataacaaatt
    14881 ctctttagaa tctcgacatc taggattagt tcctagtgta gaaatgaaat cactaaggga
    14941 aaaattttat accttagcag atgaaattga acactatata gatattgaca gaataattga
    15001 aatatcagaa agcgatgaat ttgagagtga ttttgatttt caaactttga tagaaaataa
    15061 taaaataaaa gaacatgtta ataataaatc aatagctata gcatatgata aagcattcaa
    15121 tttttattat agagaaaata tagagctttt agaggaactt ggtttagaaa taaactattt
    15181 tagccctctt gaagatgcat cagtgccaaa tgcagattat atttatattg gaggaggttt
    15241 tccagaaata tttggaaaag aattagaagc aaatgagtca atgagaactt ccatattaga
    15301 agcacataac aataatatac ctatttatgc agaatgtgga ggactgatgt acctaggtga
    15361 gaaactattg aatcaagaag aacaagaatt taatatggtt ggaatattta gtggaattag
    15421 taagatgaca tcttctttaa agagatttgg gtactgtatt ggtgtagcca aggaaaatac
    15481 tcttttatct aaaaaagggg aatctattaa agggcatgag tttcatcatt caatatttga
    15541 aagtgatgaa aaatgtgtat attcaatgag aaaagaaaga gatggaaata tagtagaaga
    15601 gtgggatggt ggttatagta aaggaaacac tttggctaca tatcttcata ctcattttta
    15661 taataactta aattgtatag aaaatttttt gaaaaaagga aatgaataaa tgagtatttt
    15721 atctatttat ataggatatg tgacagactt aattgtagga gacccatatt cttttcctca
    15781 tccagtgaga tttattggta aacttataaa ttttactcaa ggtataataa gaaaggtctc
    15841 taaaaatgat aggcagttaa aatttggggg atttatatta tggtttataa ccgttggaat
    15901 aacatattta acaacttatg ctatagtgaa gctatttagt tttaatatat tattgagtat
    15961 tgtagtgaat agttttataa tttatacaac actagccaca aaatgtttaa aagacgaagc
    16021 attaaaaatt tataatgtat taaaaaccga agatatagaa aaatctagga ttcagctatc
    16081 ttatatagta ggtagggata ctactaattt aagtgaacct gaaataataa gagcaactgt
    16141 agaaactgtt gcagaaaata ctgtagatgg tataattgcg cctatgtttt atgcatttat
    16201 tggaggagca ccacttgcta tggcctataa agctataaat acattagact caacagttgg
    16261 atataaaaat aaaaagtata aagatattgg ttttgcatct gctaagattg atgatatagc
    16321 caattatata ccagctagaa tatctgttat tcttatgaca attggaagtt tctttttaaa
    16381 ttataactat aggaactgtt ttaaaatctc tattagagat agaaagaatc ataaaagtcc
    16441 aaattgtgct ttttcagaag gcgcagtatc tggtgctcta ggaatacaac taggaggtac
    16501 caacgtttat tttggggaaa aagtatataa gccaacaata ggggataagc ttagggagat
    16561 agataaagaa gatataatta aaactaataa aattatgtat gtatcttcat ttgtatctat
    16621 gttggtattt acactaatac atgtgttatg taggatgatt ttaattaact taattatata
    16681 aattattgtt tgctaactta agggggacat agatgaagga tttagggcat ggtgccaatg
    16741 taggtgaaat ggcaagatta tatgggaaaa acccaaaaga aataattgat tttagttcaa
    16801 acataaatcc aaatgtatta ccaaatttag agagatacat tttaaaagga ttagaagagt
    16861 gtaggaatta tccagatata aattatacaa acttgagaga aaatatatca aaatatatag
    16921 gtataaatcc agactttata atacctggaa atggtgcaac agaagtgatt tatttgctta
    16981 tgaagagtgt taaaaaaaaa ttggctataa taaatccgac tttttcagag tatagaagaa
    17041 gtgctgagtt aaataattta gatattatag atttagagct agatttagaa aataacttta
    17101 aacttaatat agatataata aaagaaaata taaaaagatt tgatagttta tttatatgta
    17161 atcctaataa tcctagtggt aatgtccaag atttaaagga acttgttcat ttactaggta
    17221 aacataataa ggtactaata attgatgaaa ctttcatgga atttgttgaa gatgaaagta
    17281 aatacagttt ggttaaatat atagaatcaa ataaaaatat atttataatt aaagctgtga
    17341 ctaagttttt tggtatgcct ggtttaaggc taggatatgg tcttacaagc aacactgaaa
    17401 ttatgaacaa aatttatgaa cacaaagagc catggactat taattctttt gcagatatac
    17461 tatccaattt tatttttgaa gataaagagt atattaaaaa tagtaaagaa tattacatag
    17521 aagaaagaaa atatatgtta caagagttga gaaacataag aaatataaag gtctatgata
    17581 cagatgctaa ttttattttg ataagagcat ataaaaaaac aactaaagaa ttgaaaaaag
    17641 atttgcttaa gcaaggaaac atattagtta gagatgcgtc taattttata ggattagatg
    17701 atagttttat aagagtggct ataaaatctc atgaagataa caaaattctt atagaaaata
    17761 tgaaaaattt attgggtgat taaaatgaaa tcttatggaa tatgcccagc atcttgtgga
    17821 gaatttgtac aaggtataat tgatgatgaa gaatatttat gttcatatgc aatagatatg
    17881 tattctgaag tgtatattga agaaaaatta gtagatataa atttagggag atataagtca
    17941 aggttagcaa ttgaaaaggt atttgaaaaa tttaatttac ctaaaaaata tactaaaaat
    18001 atttctttaa atataaatag taaaatacct gttggaaaag gaatggcaag ttcaactgct
    18061 gatataggag ctacaataaa ggcaacatta tcattgatag ataaagattt aagtagtgag
    18121 gagatttcta aattagcagc agaaatagaa ccaacagatt ctatatttat agataaaaat
    18181 agtatattta atcctctaaa tggaactgtt ataaaatacc taggaaatct gaccaatgcg
    18241 aaagttataa tacttgaacc aaacaaagta ctagatacta tgaaaattag attgaggcaa
    18301 gattataata aattaaaagt agaaaataaa gaagtaataa aaaaatcatt tgctctttta
    18361 gaagaaggat tgaaaaaaaa taatttatct ctagttggag aggcttgtac tttgagtagc
    18421 ttagcaaatg agaatataga aaaaaaagag tatttaaatg aaataataaa aatatcaaaa
    18481 aaatatggag catatggagt aaatattgct cacagtggaa ctgtagtagg tattttaatt
    18541 gataagtcta tgaatgataa aaagatgata gataccttat gtgaatctaa cataaattct
    18601 gtctataata aaatttatac acaaaacata attaatggag gaattaaggg ggaaatagaa
    18661 tggaatacat aaagaatcct atgaaaatag aagaaaaaag ttttgaattg atacaagaaa
    18721 taatagacga aattagacca gattataaat ttaagaataa tattgaagaa aaaataataa
    18781 aaagagctat acatactact gctgattttg attacttaga tatattaaaa atatcagaag
    18841 aagcagttga tagtataata gatgcattaa aaaataatgc aagtatttat actgatacaa
    18901 atatggctct tagtggtata aataaaagaa agttagaatc attaggatgt aagtataaat
    18961 gtttagtggc agatgatgaa actgtaaaat tagctaaaga aaaaggaatt actcgttcta
    19021 tggcagcagt agaaattgca gctaaagaag aaggaagaaa aatattcgtt ttaggtaatg
    19081 ctccaacagc tttatataaa gttatggaga tgaaatcaga aggtagatta gatttagatg
    19141 cagttatagg agttcctgta ggttttgtag gagcacaaga atcaaaagat gaagttgaaa
    19201 aaacagatat accatatata atttctaaag gtagaaaagg tggcagtaat ttagcagcag
    19261 caatagtaaa tgctatatta tatactatgt aggtacctat atggaagaat atgtatatat
    19321 agatggaaaa aaatatagaa gaggatatac tacaggttca tgtgcaacag gtgcatctaa
    19381 ggctgctgta tatatgttga ttacaaaaaa tagaatcaat actataaata tagatactcc
    19441 taaggggata ccattactac taaaagttga taatataaat atttctgata catttgtgga
    19501 atgttccata aaaaaagatg gtggagatga tatagatgca actcatacca tggatattta
    19561 tgcaagagct gaaattgtag ctaaaaatga taagaacaaa gggtatctta ctctaaagga
    19621 tatagatagt ttaagcacca atagtgagtg taaaagtgag ctctataagt ttattagggt
    19681 ttatggcgga actggtattg gtgtggttac gaaaaaaggg cttagtgtgg atgttggtaa
    19741 acctgctata aatccgactc cacttaaaat gataaaccat gaaataagaa agcttatagg
    19801 agataatttt gaatctatac ttggaaatga taaagtttta aaaataacaa tctttgctcc
    19861 acaaggagaa actgtagcaa aaaaaacatt taatccaaga ttaggtatag ttggaggaat
    19921 atctataata ggtacaactg ggattgttga acctatgagt gatgagggtt ggaagaagtc
    19981 tctttctata gaacttcaga tgaaaaaaga acaaggctta gataaaataa tattggttcc
    20041 aggaaatcat ggagaacagt ttataagaga aaaattaaac ttagatatta agtatgtagt
    20101 tagggtaagt aattttatag gctatatgat taaagaagct caaagaatag gatataaaaa
    20161 aatcttaatg gcaggtcata taggtaaatt tataaaagta tctgctggta tttttaatac
    20221 tcatagtaag gtagcagatg ctagaagtga gatattagtg gcaaacttag ctttaatggg
    20281 agctagatac gaatttttaa ataaaataaa tcaatgcgta actactgaag aagctgtaga
    20341 gctaataaat aatagtgaat atagagaagt atataatata ttaagtaata aatgtaagga
    20401 gagagtaaag caatacttga atgaagattc agatgatatt gatgtggaag taatcatatt
    20461 ttcaatggat aagtctttat taggtaaatc ggataataca gatgatttag tggaggtctt
    20521 tatatgataa acataatagg tctaggacct ggtaacttag actatataac aaaaaaaggt
    20581 gaaaatttaa tttccacttc tgatgtatta attggtggta aaagaaatct agagtctata
    20641 aaaaattttg aaggagaaaa aatagttctg gattctaatt taagagaaat tatagagtac
    20701 ataaataaca ataaagaaaa gcaaatatca ataatagcat ctggggaccc attaatatat
    20761 gggataggta gatatctatc taaaaatatt gataataaga tgttaaacgt ggtttctggg
    20821 attagttcat tacagtacat attttcaaaa atatatgtag atatgaatga tgtatatatt
    20881 acaagtagtc atggcaaaat accagatttt gattacatat tatcacataa aaaagtatgt
    20941 atggttacag attcaaagat aggaccaaaa caaataagta gagaaataat agatagaaat
    21001 ctaaataaaa ttattgttgt aggagaaaat ctgtcatatg acaatgaaaa aataacaata
    21061 gcgaaaccag aagagataat aagaatggac aattttgata tgaatgttgt ggtgatttta
    21121 gatgaggaat agtgaattta taacaggtaa agttccaatt actaaggaag aggttagagc
    21181 aatatcaata agtaagttaa atttagtaaa tgctaaaaac ctcatagaca taggggctgg
    21241 tacaggaagt gtaagtgttg aagctgcata taattatcct aatttaaagg ttatatctat
    21301 agagaaaaat gatgacgcta tagagcttat agagaaaaat ataaaaaaat tcaacttaaa
    21361 aaatatagaa gtgataaaag gatatgctcc aattaatcta gggttaaatg ttaaagttga
    21421 gtctattttt cttgggggaa caggaaataa tctggaagaa ataatagaat ggtctaaaaa
    21481 aaatctcgtt acaggaggaa gacttgtagc taattttata ataatagata catttaatca
    21541 aactttaaaa ctgcttaaaa agcatggatt taaagaaata gatgtatgtg ttttgaatgt
    21601 ttctaaacta gagaaactag gaaaaggtga atattttaaa ccactaaatc ctatatatat
    21661 aatatcttgt gaaaaagggg aaaatgatga tgaataaagt acattttgta ggagcaggac
    21721 ctggagataa agagctgata acattaaaag gatacaaatt attaagtaat gcagatgtag
    21781 tgatatatgc aggttcactt gttaatcctg aacttttgga atactgtaaa gaagattgcc
    21841 aaatacataa tagtgcacat atggatttac aagagataat agatgttatg agagaaggta
    21901 tagaaaataa taaatctgtt gtaagactgc aaacaggtga tttttctata tatggttcaa
    21961 ttagagaaca agtggaagat ttaaataaat tgaatataga ttatgactgt actcctggag
    22021 taagttcttt tttaggggca gcatcatctt taggggttga atatacagtt ccagaaattt
    22081 ctcaaagtgt aattataaca agaatggaag gcagaactcc ggttccagag aaagaatcaa
    22141 tacaatcata tgctaagcat cagacatcta tggtaatatt tttatctgtt caagaaattg
    22201 agaaagttgt gtctaaatta ctggaaggtg gatatccaaa agatactcct atagcagtta
    22261 tatacaaagc aacttgggca gatgaaaaaa tagttaaagg aactttaagt gatatagctg
    22321 taaaagttaa agaaaacaac ataaataaga ctgctttaat aatggttggg agatttttag
    22381 gagaagagta taataattca aagttatatg ataaggattt caaacatgaa tatagagggt
    22441 aatattgcta taatctgtat tacagaaaat ggaaaaaatt tagctgtaaa gataaattct
    22501 ctagtaaata actgtgttat atatcaagta aaaaataaaa aaagtaattt agttgttgaa
    22561 gaaacatgtg tacatacagt tcaaaagaag ttaagtgatt ttgtaggaga aatatttagc
    22621 aaatttgatt acataatatt tataatggct acaggtatag ttgtacgaag tatagcacca
    22681 tacataataa gtaagtttag tgaccctgca attttggtaa tggatgaaaa aggcaaaaac
    22741 gtaataagtt tattgagtgg tcatattgga ggggcaaaca gcttgacaca gtacataagt
    22801 agtcttattg atgctaatcc agtaataaca acagctactg atataaatca aaaagcttcc
    22861 ttagatatga tagcaaaaaa actaaatgca catattgata attttagaga aagtgtaaaa
    22921 gaagtaaatt ctatgctagt gaataataaa tgtgttggat tatatgttga tgaaggatat
    22981 caagttgata ctaggggatt taaacttata gaaagtaagg atgagttaag agatagcttt
    23041 tcaaacacat atatagaaaa attagttgta gtaagcaata aaataaattg tattgaagat
    23101 attgagaatt taaacttaga taataaattt gaacattgct atagtactaa aaaaataata
    23161 aaggtaattc caagagatat agtgatagga attggctgta gaagaaacac agatagtgag
    23221 ctgatgtata attcacttga agagtttctt aataaacaaa atatagatat tagagcaata
    23281 aacacagttg gaagtataga actgaaaaaa gacgaaaaag caataattga attatcaaaa
    23341 atactaaaag tgccatttaa aacattttca atagatgaaa tatctaaggt tgataatctt
    23401 tttgataaat ctgattttgt taaaaaaaat gtgggcgtgt attcagtagc agaacctata
    23461 gcatatcttt tgagcaaagg taatttgtta gttgaaaaac ataaatataa aggaataact
    23521 ttttcagtgg ggagaataaa aatatgatat acgttgtagg aattggacca ggaagtaaag
    23581 atacaatgac tttagaagca ataaaagcaa tagaagattc tgaggctatt gttggatata
    23641 agacttatat aaaactaata gaagaattta tacaaggtaa agaaataatc caaaatggta
    23701 tgaggcaaga agttgataga tgtaaacaag cagtagagga agcgaaaaaa ggaaaaaaag
    23761 tagcagttgt aagcagtggt gatgctggaa tatatgggat ggcaggtctc attttagaat
    23821 taatatctaa agaagatgaa gatataaaag taaaggtaat tccaggtgtt actgcaagta
    23881 ttggtgcagc agctatactt ggagctccta ttatgcatga tttctgtcat ataagtttga
    23941 gtgatttaat gactccgtgg gaagtaattg aaaaaagatt gagattggct gctgaagccg
    24001 attttgtaat ttgtttgtat aatccaagaa gtaagggaag aagtgaacat ctaagcaaag
    24061 catttaaaat aatgggtgag tttaaaggtg gagatacacc tgttggagtg gtaaaagatg
    24121 taggtagaga aaaagaagaa aagtttgtat gtacttttga aaatatggac tttgaaaaag
    24181 tcgatatgac tacaatggtt ataataggaa ataagtctac ctatattaat ggtgaattga
    24241 tgataactcc aagaggttat acagtatgat tttaatttta ggaggaactt cagatagttt
    24301 atctatatgt gataagataa atgaactgaa aaatcaacca tatatccttt cagttactac
    24361 agattatgga agggaacttg cacaacaaca tgcagagaat gtaattttag gtaagcttgg
    24421 aaaagaagat atgctaaaat ttataaaaga taataatata gtaaaaataa tagatgccac
    24481 tcatccttat gcagttgaag tatccaaaac tgctattact tgtgcaaaat tattgaatat
    24541 agattatata aggtttgaga gaaaatcttt aattgaagaa atagattatg ataataaatt
    24601 tatagtagat acaattgaag aagcctgtga aatagcaaat aaaataggga caaatatatt
    24661 cataggaaca ggaagtaaaa atttgaacag atttatagag gaaataagtg ataaaaattt
    24721 agttgttaga gttcttccaa cgagtgaagt catattaagt tgtgaaaaat taggtttaag
    24781 tgcagataat atagtagcta tgaaaggtcc atttagccag tatataaatg aagaaacata
    24841 taaacattac aatatagatt tagtcataac aaaagaaagt ggaatagctg gtgggttctt
    24901 agagaaggta aatgcatgta aaactttcaa tataccagta ataattataa aaagagaatc
    24961 gattaactac gaaaatgtat ttaatgacat aaataaaatt aaagacatac ttgtgtaatt
    25021 tttaggagga gaatatatga agaaagcctt attagtagtg agttttggta caagctatca
    25081 tgaaacgaga gaaaagacaa tagatatatg tgaagataaa ataaaaaatt cgttaaaaag
    25141 ccatgatttt tttagagctt atacatcaaa tatgataata aataaaataa aaaaaaggga
    25201 tggaatagaa atagataatc caatacaggc tttagataaa atatatgaac aaggatatga
    25261 tgaagtaata atccaaacac ttcatattat atgtggcgaa gaatttaaca aattaaaaga
    25321 acaagtagat ggatattctt ctaaattcaa aaagattgta ttaggaagac ctttgcttac
    25381 acatattgaa gattatcagg aagctgtaga ggcaattaag catcaaattc ctcatatgga
    25441 aagtaatgaa gcagtagtat ttatgggaca tggaacactt catgaatcac attcagcgta
    25501 tccagcattg gaatacatgc ttagggataa tggaataaat gcttatgttg gtacagttga
    25561 agggtatcca gaaatagaac atgtaataag aagattaaaa gaaggaaata taaaaactgt
    25621 aaatttgatg ccatttatgt tagttgcagg agaccatgct ataaatgata tggcaggaga
    25681 tgaagaagat tcttggaaaa caatattaga agaagatgga tttgacgtaa gagttcattt
    25741 aaaaggacta ggagaaaatt cttatataca agataaattt gttcgtcatg ctgtaaaatg
    25801 tgcagagaat gctaagttct atggtatagg agcagggcca ggagatggtt ctttacttac
    25861 gataaaagcg gttaatactt taaagaaact ggatattcta tacactccag aatcaaaaaa
    25921 gggtggagat agtctagctt tatcaatagt tagtgaatat ttacctgaat cagtggaaat
    25981 aaagtctagg catttcccaa tgagttttga tgggaatgaa aaaagtttag cttgggacaa
    26041 tgtagcacaa gaaattatat cagatgttaa tgatggtaaa aatgtaggat ttgttacatt
    26101 aggagaccct atgatttata gtacatatgt atatataatg aaaagaatat taggagaggt
    26161 agaagtcgaa actataccag gaatttcatc attttccaat atagcatcta atcagaattt
    26221 tccattagtc atggataaag aagctttagt ggtaattcct tgtactatgg aagatgataa
    26281 gatagagtat gctttgcaaa attataattc aatagtattg atgaaggtat ataaaaattt
    26341 taaagaaata ataaaaaaat tagaaaagta tgatttaata gaacatgcta ttttagtaag
    26401 taattcttcc caagaaagcg aaaatgtgtt tacagattta aaagaagcgc accttgaaga
    26461 taagatatct tatttttcta ctatactagt aaataaagat aataaaataa aataggtgaa
    26521 agcaatattt aaaggagact taatatgaat atagtggttg gaactagggg tagtactcta
    26581 gcgcttatac aaacagagtg ggtaataaat gaattaaaaa agaagtatcc agaaattagt
    26641 tttgagatta aaatcataaa gacaaaagga gatttaattc aaaatgtttc actggataaa
    26701 ataggtgata aaggattgtt tgtcaaagaa atagagcaac aattattaga tggaaaaata
    26761 gacatagcag tacatagtat gaaagatatg ccttcttatt tagcagatgg actaaaattt
    26821 gctcatacgc ctaagagaga agacccaaga gatgttctaa tacttagaga aggatataaa
    26881 aatttagatg atttgcctca tggagcagta ataggaacag gaagtaaaag aaggaagttt
    26941 caattattga aacaaagacc tgatttaaat atagtacaag ttagaggtaa tgtggaaact
    27001 aggatacgaa aaataaaaga tgaaaacatg catggtattg tgttggcagc atcaggaata
    27061 attagagcta atttgcaaga taaaataagt tcttatttac ctgttgatgt tgttattcca
    27121 gcacctgccc aaggagcttt agctatagaa attagaagca atgatagtac gatagaggga
    27181 atagtaaatt cactgaagga tgagaataca gaaatccaaa tattggctga aagaggtttt
    27241 ttagatggtg taaatggaag ttgtcatatt ccaatggcag cttattgtga aataatccaa
    27301 gataaaatac atcttactgg cttatatgga gattcagaag gaaaaaaagt agtaatcaag
    27361 tctatagatg gagatatttc ttctcctaga gagttaggtt taaagttagc taaacttgta
    27421 ttgaaggagt atgagaatta tgaagggtaa ggtttactta gtaggggcag gaccaggtga
    27481 ttataaactc atgactttaa aaggtttaga gtgtataaga aaatcggatg taatagtata
    27541 tgatagatta gcaaatagta gttatttaag agaagctaag cctgattgtg aacttatata
    27601 tgtaggcaag gcatcaagta atcatatttt gccacaagaa gatataaata gaattatagc
    27661 agaaaaagct aaagaaggaa aaattgtaac tcgtcttaaa ggaggagacc cttatgtatt
    27721 tggcagaggg ggagaagaag cagagacttt agtagatgaa gatatagaat ttgaagtagt
    27781 tccaggtata acttctgcta taggaggttt atgttatgcg ggtattccta taacacatag
    27841 agaccatgct tcgtcatttc atgtaataac aggtcatttg aagggtgatg atagtggaga
    27901 attaaactgg aatgctcttg caaataacaa aggtactctt gtgtttttga tgggaatttc
    27961 aaacctagag aaaataagtg aaaacttaat gaaagaagga aaagataagg atacacctgt
    28021 tgctcttata agttgggcaa ctagatataa tcaaagagtt gttacatcta cacttgagaa
    28081 tgtttatgaa actgctatta aagaagaagt aaaaccgcca actcttatag tagttgggag
    28141 tgttgtagga cttagagaaa agttgaactt ctttgaaagt aagcctttat ttggaaaaag
    28201 tattgctgta actaggtcta gaaatcaaaa tagtgtattg gtagaaaaaa taatggattt
    28261 aggtggaaat cctatagaaa tacctactat taaggtagaa aaaatacaaa ataatattaa
    28321 cttagaaaat gaaataaaaa atataaataa gtataattat ttaatactaa ctagtaaaaa
    28381 tgccgtagaa atcttctttg aaaagatata tgaaatgaat tttgatttaa ggatattatc
    28441 taatttaaag gtatgtgcta taggttctgc tacatcaaat gagttgaaaa aaagaggaat
    28501 tatagcggat atagtaccaa aaaaatttgt agcagagtca ctgtatgaag agttagctcc
    28561 tattttaaat gataacgata atatacttat acctagagca gaaaatgcta gagattatct
    28621 ggttgacaaa cttaaggcta tttgtgaagt aacagaagta catatatata gaacagtaat
    28681 agatgaggat aaaaaaggtg agatattaga tattttaaat tctaatgata tagattatat
    28741 aacatttact agttcctcaa cagtaaataa tcttgttgaa attataggaa aagaaaatat
    28801 taaaatatta gaaaaaataa aaacactatc tataggtcct ataactacag agacaataaa
    28861 tagtttggga ataaaattgt acaaagaatc tgctacatct actatagatt cgttagtaga
    28921 tttaattata aaaaattaat tttggaggct tgaatatgtt aagaagacca agaagactaa
    28981 gagcaagtaa agcaatcaga aatttggtaa gagaaactaa ggtgaatgta gaagatttaa
    29041 tatatccact gtttgtagta gagggggaaa atataaaaga agaaatcaat tctttaccag
    29101 atgtatatca tttatcaata gacatgctag aagacgaaat aaaagaaata agtgagttgg
    29161 gaattgaaca tataatgtta tttggtgttc cagatgaaaa agatgagtgt gcttcacaag
    29221 cttttaatga taatggaata gttcaaaagg caattagaaa ggtaaaagaa ataaattcag
    29281 atataaatgt aataacagat gtctgtatgt gccaatatac aagtcatgga cattgtggga
    29341 ttttaactga tactggttat gttgataatg ataaatcatt ggagtatttg gctaaaatat
    29401 ctgtaagcca tgctaaagca ggagctgata tggttgctcc atctgatatg atggatggaa
    29461 gaatacaagc aataagagaa gctttagatg aaaatggatt agaaaatgtt ggaatcatgg
    29521 cttatagtgt taagtatgct tcaaattatt atggaccttt tagagaggca gctcattcag
    29581 caccatcttt tggagataga aaaacatatc aaatggaccc agcaaatagc aatgaagcta
    29641 taatagagac tgaacttgat atattggaag gtgcagatat acttatggtt aaacctgcat
    29701 tatcatattt agatattata agaagagtta aagataattt tgacttacct ctagcagctt
    29761 ataatgtaag tggagaatac tctatgctta agtttgctgt gaaggaaggt ttattaaatg
    29821 aagatgtaat tcttgaatca attacatcta taaaaagagc aggagcagat ataataataa
    29881 cttattttgc taaagacatt gctaaactgt tgaaaaaata aattctagta gtataaagaa
    29941 tatcaaaaag tataggaatt gtaattaact attatacagt agttttgttt agacatttat
    30001 tgaagaataa tagaaactaa tttacaattt ctatacttta tttatgtaac taaataaaat
    30061 ttggaggtag aagatgctat atcctattaa tttaaaatta gatgagttag atgtagttat
    30121 aattggtggt ggagaagttg catataggaa atgtaaaaat tttttagagt ttaacaaaaa
    30181 tgttactata gtttctaaac aaattttaaa taaattttat gatttaaaag gaaatataaa
    30241 aataataaaa gatgattata aagaaatata tataaaagat gcttctgtaa taattgctgc
    30301 cacaaataat agagagttaa atatggaaat aggattatat tgtagagcaa aaaataagct
    30361 agtgaatgta gttgataata tagaaatatc gaactttact gttccatcat atataaaaag
    30421 aggagattta cttataagtg tgtctactgg gggcaagagt ccttcattat catcaaagat
    30481 aaaaaaagaa attgaagaga gatatactga agattatgag gagtatttaa atgtattagg
    30541 agatataaga aaaaaggtta taaaaaaata cgaagataaa agtaaaagaa aagatgtgct
    30601 taatatgttg gtaacacttg acctagaaga acttaagaag tttgatatat aaaaaataaa
    30661 ataaaaaatg ttaaaaaggt gttgacggat tatttttaag atgatatagt
//
LOCUS       DACTGW010000021        30496 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.021, whole genome shotgun sequence.
ACCESSION   DACTGW010000021 DACTGW010000000
VERSION     DACTGW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.021"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            50..844
                     /locus_tag="I9573_000763"
     CDS             50..844
                     /locus_tag="I9573_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAT4795397.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            938..2278
                     /locus_tag="I9573_000764"
     CDS             938..2278
                     /locus_tag="I9573_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HAT4795398.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            2415..3647
                     /locus_tag="I9573_000765"
     CDS             2415..3647
                     /locus_tag="I9573_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HAT4795399.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            3687..4829
                     /locus_tag="I9573_000766"
     CDS             3687..4829
                     /locus_tag="I9573_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAT4795400.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGTDIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGIGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASSTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            4840..5634
                     /locus_tag="I9573_000767"
     CDS             4840..5634
                     /locus_tag="I9573_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAT4795401.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFAPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            5645..6667
                     /locus_tag="I9573_000768"
     CDS             5645..6667
                     /locus_tag="I9573_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAT4795402.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELEVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEVLPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            6766..8142
                     /locus_tag="I9573_000769"
     CDS             6766..8142
                     /locus_tag="I9573_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAT4795403.1"
                     /translation="MKEQWYKDIFARILSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKDKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(8474..8818)
                     /locus_tag="I9573_000770"
     CDS             complement(8474..8818)
                     /locus_tag="I9573_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795404.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            9306..10316
                     /locus_tag="I9573_000771"
     CDS             9306..10316
                     /locus_tag="I9573_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HAT4795405.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEESTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            10452..10940
                     /locus_tag="I9573_000772"
     CDS             10452..10940
                     /locus_tag="I9573_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT4795406.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            11116..13044
                     /locus_tag="I9573_000773"
     CDS             11116..13044
                     /locus_tag="I9573_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT4795407.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFILSNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            13219..13629
                     /locus_tag="I9573_000774"
     CDS             13219..13629
                     /locus_tag="I9573_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAT4795408.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            13651..13953
                     /locus_tag="I9573_000775"
     CDS             13651..13953
                     /locus_tag="I9573_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HAT4795409.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(14169..14861)
                     /locus_tag="I9573_000776"
     CDS             complement(14169..14861)
                     /locus_tag="I9573_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAT4795410.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(14887..16074)
                     /locus_tag="I9573_000777"
     CDS             complement(14887..16074)
                     /locus_tag="I9573_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4795411.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            16410..18287
                     /locus_tag="I9573_000778"
     CDS             16410..18287
                     /locus_tag="I9573_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAT4795412.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            18599..20005
                     /locus_tag="I9573_000779"
     CDS             18599..20005
                     /locus_tag="I9573_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAT4795413.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            20033..20881
                     /locus_tag="I9573_000780"
     CDS             20033..20881
                     /locus_tag="I9573_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAT4795414.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            21542..22234
                     /locus_tag="I9573_000781"
     CDS             21542..22234
                     /locus_tag="I9573_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4795415.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            22231..23166
                     /locus_tag="I9573_000782"
     CDS             22231..23166
                     /locus_tag="I9573_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4795416.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            23314..24237
                     /locus_tag="I9573_000783"
     CDS             23314..24237
                     /locus_tag="I9573_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4795417.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            24230..24991
                     /locus_tag="I9573_000784"
     CDS             24230..24991
                     /locus_tag="I9573_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4795418.1"
                     /translation="MLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNLPFE
                     QLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLAVLL
                     FISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYFNRS
                     YIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEYTAL
                     RQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            25001..25783
                     /locus_tag="I9573_000785"
     CDS             25001..25783
                     /locus_tag="I9573_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4795419.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            26157..26696
                     /locus_tag="I9573_000786"
     CDS             26157..26696
                     /locus_tag="I9573_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HAT4795420.1"
                     /translation="MMNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAA
                     IFEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGF
                     DKIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPE
                     KCPVCDHPKAYFNIKAENY"
     gene            26728..27117
                     /locus_tag="I9573_000787"
     CDS             26728..27117
                     /locus_tag="I9573_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAT4795421.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            27154..27540
                     /locus_tag="I9573_000788"
     CDS             27154..27540
                     /locus_tag="I9573_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HAT4795422.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            27667..29109
                     /locus_tag="I9573_000789"
     CDS             27667..29109
                     /locus_tag="I9573_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HAT4795423.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            29406..30221
                     /locus_tag="I9573_000790"
     CDS             29406..30221
                     /locus_tag="I9573_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT4795424.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT        11289 a         3594 c         5743 g         9870 t
ORIGIN      
        1 attaacaaga aatgtagaaa aaataacaat gtataatggg gggaatatga tggaatctgt
       61 aatggaaaat ttgaacaatc ttaaggtgga ggttaaggac aaagtctgtg ttattactat
      121 aaatagacct aaagctttaa atgctcttaa tagtgacact cttagggaac tatcacaagt
      181 tattgatgta gtatcagaaa atgaggcaat attaggagtt attattactg gagaaggtaa
      241 agtttttgta gccggtgctg atatacgcca gatgcaaaac tataaaagtg aggaaggcag
      301 aaaatatgca ggttatgctc agggaatttt tgataaaatt gaagctcttg agaaaacagt
      361 aatagcagca gttaatggat atgcattagg tggtggatgt gaacttgcta tgagttgtga
      421 tattagaatt gcatcagaaa aggctatatt tggacaacct gaagttaatt taggagttat
      481 tccatgtttt ggtggaactc aaagattatc aagacttgtt ggaactggaa tagctaaaga
      541 gttaattttt acggggagac aagtaaatgc agaagaagct aaatctattg ggcttattaa
      601 taaagtggtt ccaagtgatt tacttttaga agagtctatg aagatgatga accaaatagt
      661 tgaaaaagcg ccaatagcaa taagatatgc aaaagttgtt attaacaaag ggatagatat
      721 ggatttaaag aatgctcttg aactagagaa agatattgca ggtttaactt ttgctacaag
      781 agataagcaa gaaggaatga atgcatttat agaaaagaga aagccagttt ttgaaaataa
      841 gtagctatta taactaaatt ggaacaatgt agaatatatt caaaatctaa atttcgtgaa
      901 aaaatacata tagtttaatt ttaaaaggag ttaatttatg aatacgggag taattataag
      961 tttagtaggg attatcatag ctctatctct tctagttgta atggttatga agggaataaa
     1021 tatttttatt atagcaattg tatgttcatc attagttgct ataacaggaa gcctaaatgt
     1081 gtatgatgca cttaaagtgg attatatgac aggatttgtt ggatttcttc aggcaaattt
     1141 ctttattttc ctaacaggga cattaatggg aaaaataatg gagattactg gtggagcaaa
     1201 atctattgct aagatgattg taagatggat tggtaaggat aaagcactac tttctattcc
     1261 aatagcttgt ggaatattag cctatggtgg agtaagtgtt ttcgttgtaa gttttgcagt
     1321 gtttccgata gctttagaaa tttttaaaga agctgatttg ccacgaagat ttataccagc
     1381 agctttgact tttggatgct caacttttgc tatggttgca ccaggggcac cacaaattca
     1441 gaatattgtt ccagcatcaa cattagggac agatattatg gccggagcag taaatggttt
     1501 tatatcatgt gcagtaatgt ttatagttgg aagtaccatt ttgtacaaaa tggtttcaaa
     1561 agaaaaagcc aatggtggtc attttatagc acatgaatca gatatatttg atgaagcaga
     1621 agcaactaat tctaagagag aaaatggtcc taatggtgta attgccttaa ttccattgat
     1681 agtgtcaatt ttaataatta atgtaaaagt taatggaaaa gcgatagtac caattgaggt
     1741 gggagtattt attggagctt tattagtata tttattgtta aataaatatc aagataatac
     1801 taaaatagtt ggacatgttg gtgatgcatg taaaactaca gtagtagcaa tatgtaatac
     1861 ttgtgctgta gttgcatttg gttcagtagt aaaatcagca gttggttttg acttcatagt
     1921 taatgccatg actaatattc ctggtcctcc tattgcaggg gctgcagttg gaactacagt
     1981 tattgcaggt atttgtgggt cagcttcagg tggactagga attgcagctc cacttcttgg
     2041 accagtattt ttagccaagg gtgtttccgc tgcagcatta actaggacta tggcaatttc
     2101 atcagcagca ttagattcac ttccacacaa tggatatatt gttactgtaa caaatggtct
     2161 gtgtaaagaa acacataagg atgcctatat gccaatattc tgggtaactg ttttaactcc
     2221 gtttattgga accatagtag cagtcctact gtttacaatg ttcccgggat tgccataaga
     2281 gaaaagataa aaggtataaa ttatattttg atattataaa atcaattttc aaaatatata
     2341 caataaattt catgttttaa tactataaat atgtatttaa aataacaaat tgaccatata
     2401 aggaggaatt gaaagtgtta aataaaccat tagagggagt taaggttatt gacctaacgt
     2461 attttgtagc aggtccaggg acttctaaaa tattagctga ttggggtgct gacgttatta
     2521 aggtagagcc aagctttgga gacccaggaa gaaagacagg ggcaacaatg acaatgccaa
     2581 tagatgatta taataatcct ttttacagca catataattc aaataaaaga ggtttgtcta
     2641 taaatcttaa gagtgaaact ggaattgaaa ttatggataa gcttttaagt gaagcaaacg
     2701 tatttgtttc aagttataga acaggagcat taaaaagact aggtcttgat tatgaatcat
     2761 taagtaaaaa gcatccacac cttatctggg gtcagataaa tggttttggc gattttggac
     2821 ctgctaagga taatgcagga tttgatacag ttgccttttg ggcaagaagt ggggctatgc
     2881 ttgatatagc agaaaaagat acaagtccaa taaatccacc tatagccttt ggagatgcaa
     2941 caacttcttg tagtttatct ggaggaatat gtgcagcttt gtaccagcaa gctaagactg
     3001 gaaaaggaca aaaagtaatg gcatctttat ttgggcaagc aatatggaat gcatcatctt
     3061 tattagcatc tacacagttt agtgatgagt atccaaaaac tagaaaaaat gctatttcac
     3121 cagtaataaa ttcttatcgt tgtaaggatg gaaagtggat attcttaagt attttagagc
     3181 atgaaagata ttttgaggca ctttgtagat tatttggaag agaagactta ataaaagaag
     3241 agaaatttgc atcttcagta gaaagtaaga aaaatgctac agaacttatt aatattttgg
     3301 atggtgaatt tgcaaagttt actcaaaatg agatggttga aaaacttact gctgctgata
     3361 tagctcatga aaaaattcaa catgttaatg atgtagtgac tgatgaacag gctattgcta
     3421 ataactatat atatgagcat acaagtaaaa atggaaataa gacaatgctt gcatccactc
     3481 cagttaaatt tggaaatatt gaagttaaca tgacttgtga tgctccactt ataggagaac
     3541 attcagatga gatattgaga gaacttggat acagtgattc tgatatagaa gctctaattg
     3601 aatctggaat tgtaacaatg aataaagaag catgtgaaat atgttaataa acatgtgaag
     3661 tatgttaata aataggaggg aattttatgg attttagatt aacagaagca caattaatgc
     3721 ttcaaagagt agcaaaagaa tttgcagaga atgaaatagc accaatagct gctgaaacag
     3781 ataaaacagg tatttttcca agggagcttt ttagcaaaat ggctaaaata ggatttaatg
     3841 gcattggaac acctgtagag tatagtggtt ctggagggac agatatagaa aaggtaattg
     3901 ttgttactga gattgccaaa aaatgtgcag catctgcagc aatattatca atacacacaa
     3961 tatatgctca agctattttg aaatttggta ctgaggaaca aaagaaaaaa taccttccaa
     4021 tgatggctga aggtgggtgt gtaggtgctt tcgccttaac tgaacctaat gctggttcag
     4081 atgctgcaag agctgcaaca actgcaatta ttgatgaaga aacggatgaa tatgttttaa
     4141 atggaactaa gtgttttatt tctggtggag gtcaagcaga atcattaata atatttgcac
     4201 tgacagaccc atcaaaggga ataaagggaa tgtctgctat cattgtcgac aaaggtactc
     4261 caggtttttc aataggaaaa attgaagaaa aaatgggtat tcatggttca gaaactgcag
     4321 agttaatatt tgataactgt agagttccaa agtctaactt attaggaaaa gaaggcaaag
     4381 gatttaatat agctatgact tgtttagatg gagcaagaat tggaattgga gctcaagctg
     4441 taggaattgc tgaaggggct ttagaagaaa gtatcaaata ttctaaagaa agagtccagt
     4501 ttggcaagcc aatttcagcg ttgcaaggta ttcaatggta tatagcagat atggcgacaa
     4561 tggtggaatc agcaaaatta ttagtatatt atgctgcaga tttgaaagct agaggagaaa
     4621 aacacactaa agaagctgct atggcaaaat acaatgcgtc aagtactgca agagaggtta
     4681 caaatctagc ccttcaaatc catggtggat atggatatat gaaggattat ccattagaga
     4741 gaatgtacag agatgccaaa ataacagaaa tttacgaagg tacttcagaa atacacaaag
     4801 ttgttatttc aagagcagta ctaggatagg aggaaaagaa tgaatatagt tgtttgctta
     4861 aagcaagttc cagatactaa tgaagttaaa ataaataaag agacaggtac tctaataaga
     4921 gatggtgtac caagtataat caatccagat gatagaaatg ctttagaaga agctctaaaa
     4981 atgaaagatg aactaggagc agtgattaag gttataagca tgggacctcc tcaagctaaa
     5041 agtgctttaa aagaagcttt ggcaatggga gctgatgaag cttatttaat ctcagataga
     5101 gcttttggtg gttcagatac ttgggctaca tctacaatta ttgctgctgc tatagaaaag
     5161 gttggaaagt atgatgtaat attctgcgga agacaagcta tagatggaga tacagctcag
     5221 gttggaccag aggttgcaga atttttaggg ataccacaag ttacttatgc aaaagaagta
     5281 aaggttcaag atgacaaatt actagtaact cgttatacag agacagggga ttatttaata
     5341 gaagcaaaga tgccagtttt attaactgct ataaaagagt taaacaatcc aagatatcca
     5401 agtgtaaaag gaatcttaga agcttataat aatggagatg cgaagattac tgtattaaca
     5461 cttgcagatt tagatgttga tacaactcaa attggattaa agggttcacc tacaaatgtt
     5521 tataagtctt ttgcaccagt taaagacaag cataatgaaa taatagaagg tataaataaa
     5581 aaagaaaaag ctgagaaact aatagaaata ctattcgatt tgaaactagt ataggagtgt
     5641 taagatgatg agagcaaaag ttaatcaagg tataaattta aatgattata atggtgtttg
     5701 ggtaataggg gagcaaaggg aaggcaaaat taatcctgtt acgatagaac ttattggtga
     5761 aggaagaaag cttgctgacc aattaggaaa agagcttgaa gttgtgatag ctggatatga
     5821 agtagagaaa gaagtgaagg aattacttca ctatagtgtt gataaagtat attacatcaa
     5881 tgaccctctt cttaaagatt tcactactga tggatatgca atttcaattg caaatttaat
     5941 cgaaagaaaa aagccagagg ttgtattggt tggtgctact tctataggaa gagatatagc
     6001 gcctagaatt gcaggtaagg ttggaacagg tctaactgct gactgtacaa aacttgaaat
     6061 agattcaaca gataataagt tattacaaac aagaccagca tttggtggaa atttaatggc
     6121 cactattgtt tgtccaaaaa atagaccaca gatgtcaaca gttcgtccag gagttatggc
     6181 aaaagctgta agaaacgaat cagagactgg aattttagaa gttgttactc cagaacttac
     6241 tgaaaaaatg attagaacta gattagtaga ggtacttcca caagaaaaga aatctgtaaa
     6301 tctaactgat gctagaataa tagtatcagg aggaagagga ttaaaaagag cagaaggatt
     6361 tgagcttata aaagaattgg ctgataaatt gggggcagag attggtgctt caagagcagc
     6421 agtagattca gggtggatag aacattctca tcaagtggga caaacaggaa ctactgttag
     6481 accagaacta tacattgcat gtggaatatc tggagcaata caacacttag caggtatgag
     6541 tgattctaaa tatatagttg ctataaataa agatgcaaaa gcccctattt ttagtatatg
     6601 tgactatgga atagtaggag atttatatga aattatacct gaaatgatag aatcattaaa
     6661 taggtaactt ttagaaaaca ttgaatatgt agaatgataa gtcttataat attgtattgt
     6721 aagacttatt atttttgctg aaggttatag tggggagatg aaacaatgaa agagcagtgg
     6781 tataaggata tatttgcaag aatattatct atgactgatg atggttttat tgttgtaaat
     6841 acaagtggag taataataga tattaatgat aaatattgtg actttttagg aaaagaaaga
     6901 aaagatataa taggacaaaa tatacaaagt attataccaa atacaaaaat gctagatgtt
     6961 atgaaagata agtactgtga agaaggagct atacatcatt attcaggtgg aaatacaaaa
     7021 gaaaaaagtg taatagtaag taggtcttat gtagaaaatg acaatggaga agtagttgca
     7081 ggagtagctc aagttaaatt tagattgcaa tcatttgatg tagctaaaaa attaatgtca
     7141 gaatatatgg agctccaata ttataaagag cagtttaagg ataactgcgg ttttgacaag
     7201 ctcataggtg aaaatagaga cttcatagaa cttaagaaaa caggtgtgaa agcttctaaa
     7261 acaaactttc cagtactttt aacgggtgaa acaggcacag gtaaagaagt ctttgctaga
     7321 gcaattcaca ataatagcag taggtcagat aaacctatgg ttagtataaa ctgcgctgca
     7381 ataccagaag aattattaga gtcagagcta tttggttatg acgaaggagc ttttacagga
     7441 gcaaaaaagg gtggtaaaaa aggtaagttt ttagttgcaa ataatggtac aatcttttta
     7501 gacgagatag gtgacatgcc tttgaccatg caagcaaaac ttttaagggt tcttcaggaa
     7561 agtgagatag aaccagtggg aggtcttaaa actataaaga tagatgtaag agtaatctct
     7621 gccacaagaa agaatttaag taaaatggtt gaagagggat tatttagaga agacctatac
     7681 tatagattaa atgtaataaa cattcatatg atggaattga aggacaggca agatgatatt
     7741 ttattgctag caaattatat attaaacaaa ttaaatgttg aatacaagga attgaaggtt
     7801 ttaagtgata aggttaaaaa ttgttttatt aactatactt ggccgggaaa tataagagaa
     7861 ctacaaaatg taataaaaag tgcctatgct gtaagtgatg atatggtcat tatgatgtgt
     7921 gaccttccat ctaagatgga taatatcagc agagtagctc aatgtaatgt ggatagcaat
     7981 tgttcaattc atgaaatggt tgagaattat gaaaaaagct taattattga tgtgttaagg
     8041 aagtataatt ggaaatgttc taaggcagca gaggttatgg gaatacacaa gagtctttta
     8101 tataagaaaa taaaaaagta tgaaattgaa ttaaataatt aagataactt gaattaaggc
     8161 aatgctaaaa aaagcgccct tattcaaagc gaactgtata aactgtttta tgcagttaaa
     8221 acgtgtaaaa gtagatgtga agtactaatg tcaatcatta gtacttttta ttgtatttat
     8281 aattactatc tatatttcag tatcttttga tatctaagat tattatgttt aatttaggat
     8341 ataacaaaac cctcttagtt attgacagtt ttttatattt taactgtcta ctaagagggg
     8401 atagtttagt ttaaatttag agtaaatttt atctagcatt tataaaaaat attttgaata
     8461 gcttttttgt tatttagtca taattttcta cttctttatc aagttctttg ccacaagaat
     8521 cacacttata tatagcattg tatccataat catattcatc aactattttc ttttgtctaa
     8581 atacaccacc acactcacat aggaaaccaa tttttataag tgtaacgata aattgtgatt
     8641 ttataaattt tatatcatta catttatcgt tagtgtaaaa tgtataaata taagagttat
     8701 cgcactcatc tcttaattca aatctttcaa tatatgtgtt attttttaat tcctcaaatt
     8761 cttctttagt taaatatgat ttttgttgca attcatgtaa acgcttaatt tggtccatta
     8821 tttatatcct cccaataatt ttagtatttt aagcttagat aaggaatcta agttttatta
     8881 aatttatccc aaggaattat ttaaatcata tttagtttta ttaattacaa catatataaa
     8941 ttacacgaaa ataaaactaa ttgtaaaaat aatatcctgt gaaaacctaa actactaacc
     9001 ccttactaac ccgaaaacta aaaaaagaaa atatatccaa atataagtga tatataaata
     9061 aagatgtttt atataaattt aactttaaat attctgacaa taatatatat aaatatatta
     9121 tagttaaaat ataccacata ttttgggcat tgtatactgg aattagagta attaatttat
     9181 tttttaacat acaaatgatt ttatgaaaaa aaacaagact caaaaatttt tatattcttt
     9241 gatagaataa aattgagagt aaatttattg aattttataa aatttataag gggtgagtct
     9301 aagatatgca tgaagataaa aatgatatat ttgaatcaat agatactgag gattttgata
     9361 ataaaacaga agaagaatgt ttagagctag taagaaaaaa tggattgaat ttaatataca
     9421 taaaaaatca aacagaagag atatgtctag aggctgttag acaaaattgt aatgcaataa
     9481 aatgtgttca gaatcagaca gaaaaaatat gtatagaagc agttaaacaa gattggagaa
     9541 tgttagaatt tgtggaagag tcaacagaag aaatttgcat gttggctatt aaccaagatg
     9601 gaacagcatt aaaatatata gaaaatcaga cagaggagtt atgcttaaga gccgttgaaa
     9661 aaaatggtgc ggctttggag tatgtaaaag aacaaacaga agaaatatgt atagaagcag
     9721 ttagaaacag cgaatttgga ttggcaagag tgaaaaatca aacagaaaaa atatgtatgg
     9781 aagcagttaa gcattgtagc tataatctaa aatgggtaga aaatcaaaca gaagaaatat
     9841 gtatggaagc agtgagacaa aatgggcttg atttgaaatt tgtaaaaaat caaacagaaa
     9901 caatctgttt aagagcagta agacaaaatg gaatggcatt agaatttgta aaggaacaga
     9961 ctgttggtat atgcttaaag gcagtaagac aatatggaat ggcattaaaa tttgtaaaag
    10021 aacaaacaga agaaatttgt acagaagcta taaaacaaga taaaagagca cttagttttg
    10081 taaaaggaga taaggaaaaa tacaaggctc tatatgataa taatgaacca tttgcaaaga
    10141 gatatgttag aaatgtaata gaaaaagaaa acagagcatt gattaaaaaa ggagaagaaa
    10201 atgcaaaaat caaaattgca ggaaaacttt atgatagagg catgtctttt gaagatatat
    10261 cagatatagt tgagattgaa ataagcaagt taaaagtgag tttaggtgtt ttttaaagta
    10321 ataaatgtag gtgtttttta tgggaaatag ttaataaaaa aataatcgac tagctctttg
    10381 taggacagca aaattagtga tataatttga tgtataataa attattttta ttagataagg
    10441 gggaattaaa gttgaagatt acagtattat accaaacaag aagtggaaac acagaaaggg
    10501 tcgctaagct gatagaagat ggagctaaaa aggtggatgg agtagatgta aagcttatga
    10561 gacttgataa tattgattta gattatttaa atgaaagtaa agcagtaata tttggaacac
    10621 ctacttattt agcaaataca acttgggaag taaaaaaatg gtttgatgaa gattcaaaaa
    10681 aagttaattt agctggaaaa ttaggagctg tttttgctac atgtgattat atatgtggag
    10741 gtccagatgt tgcgatactt actatagtag gacatttgat ggttaaaggg atgcttgtgt
    10801 attctggagg tggttcttta ggaaagccat ttatacattt aggacatgta cattcaattg
    10861 aaggtccaga gttacaagat gagaaggcta taatatttgg tgaaagaatt gctactaagg
    10921 caaaagaact atttgcttaa tttctatatg cataataatg attagagtaa atttagggtt
    10981 ttaatggaga tttgaagtaa aagttataaa ataaaaaatt ttccataaaa ttactcaaga
    11041 tgaattataa tataattata aataaattat attattaaaa tatttaaaat caaacgtcaa
    11101 tagcgaggga agtttatggt atggcatata gaatatgaga ttttttctgc aattatagtt
    11161 atttttttaa tggtatactt ttttagaagt aaatttatac ctactttaca aaacaaaata
    11221 tattgtgcac ttttaatatt ctcattttta tttatacttt caaatatttt aggttcattt
    11281 tgtctaaata atatagacaa aattccaatt tttattactt ttttattgaa tcagatatac
    11341 ttattgttat tgccaatacc agcagctctt gtatcatttt atgttatggc tattatttat
    11401 caagatataa ggtatatgaa aaaaagatta ttatttttat ctataccact tataatttgt
    11461 ataatcttat ctattacaaa tccttttacg catattctat ttggattgtc aaaagaaaca
    11521 ggttatatac ggggttgtgg atatgttgca acttttgcat cttttttctt ttatgcaata
    11581 tattcagcat ttttatcttt taaatataga aaagcaatgc atgaatcaaa aatatgggct
    11641 attagaggtt ttcttatagt ttcaattgtg gcaattatct tacaggcaat ttttttacag
    11701 tatcttctta caggaactgc atgtgcatgt agtttacttt tgacatatct ttctatgcag
    11761 aatcgaggtc ttattattga tgaccttact ggtgtactca atagacaatc ttttattcaa
    11821 gagctagatt tgaatatcaa tactgaacaa tcaggattta ttattacaat tgcgcttgat
    11881 gattttaagt ttatgaatga aacttttgga acaaaaaatg gagatattgc actaaaagaa
    11941 gttggaaaat atttgattca gatacatgat gtaaaccatg tatatagaat agggggagat
    12001 atattttcta ttgttgtcaa tagtcaatta ggtatagaac cagatgatat tatcagaaag
    12061 atagaagaaa gatttaaaaa gccttggcta gtagaagaga taagttttaa tttagcaact
    12121 agtattgctg ttgtttatta cccagaaaat gcagaaacga cagaagatgt aattgttgct
    12181 atagatttct ctatacatga agcaaaacgt tctgagtcaa ggcgagttgt ttatgcagat
    12241 gcgtctataa gtgaaaagat aaagaagaaa catgttatta aaaattgttt aaaagaagca
    12301 ttaaaaaatg atgggtttga agtgtactac caacctatat tttctaatag caggggcagg
    12361 tttacttcag cagaggcatt acttaggcta gaacacaaag agttaggttt tatacctcct
    12421 gatgaattta ttccaattgc agaacaaaca ggattgatta actcaattgg attagtagtc
    12481 tttgagaagg tatgtagatt tatagctagt gatgagtttg aaaatttaaa attggataat
    12541 atagctgtga atctttctgt agtacaatgt atgcaaaaaa acttagctga tgatttactt
    12601 tatcttatga ataaatataa tgtatcacca actaaattta aacttgaaat tactgagact
    12661 gtggctatgg gctcttttaa tataattaaa gaaacaatgg aaagattaat tgatttaggt
    12721 gttaagtttg cacttgatga ttttggaatt gcttattctg gagttactaa tatgctttct
    12781 ttaccatttt cagtaattaa gttagataag agtttgattt ggtctatgaa tgaagatagt
    12841 aggcataagc ttacagttga gacgattata gctcttataa ataaattgaa tatgaaggct
    12901 gtagcagaag gtgttgaaac cattaagtgt gctgaagatt taatagctat ggagtgtgaa
    12961 tatttgcaag ggtattattt ttctaaacca gttccagaag atgtttttaa aaatttgttg
    13021 aaagaaaata cttttacaag ttaacaccct ttattttttt aattacaact ttgttcattg
    13081 acactgccag cgatatatta taaaattaac acaataataa atattaaatt tattatacat
    13141 attattaata ttaattaatg gagtaaagat ttattatatt ttatttaatt atcctaaagt
    13201 gagaaaggaa gatgtgacat gaataaaaag aaaatattag tgccaattga tgggacagaa
    13261 agaagtatgc attctttgga atttataaaa ggaatattta aaaaagatga agttgaagta
    13321 gaaataatga atgtaaaaga actagtattt attgatggca tatccctggc tgaagagata
    13381 aaaaattctg aaaatttagg aagaagaatc ttggataaag cagcagagat aatgggagag
    13441 tatgatgtta aagtacattt tacttttgga tatccaggag atgagataat tagaaaagca
    13501 aaagaagata atgtagattt tatagttatg acaaaatcta ctaaaaaagg tcttacaaga
    13561 atgataggtt cagtaacagc aagtgtagtt aaacaagcaa aatgtatagt gatgattgta
    13621 ccggagtaga attagaaagg tatttgaaat atgaataaaa agatagtagc agtttgtgaa
    13681 agtgaaagaa gtttaaattc tcttaagaca gctgcaaatg atttaggatt tgatttagtt
    13741 tatgaaattc aagaaaatga taatataaaa aatgaacttt ctatttatga tatagaagat
    13801 gcatctatag tgttatttgt tacaagtgat tctatagaaa gcatagagaa gattgataga
    13861 tttatagata gagaatttta tgaagttgat ccaaagtata taatagagga tgcaaaatct
    13921 atagtaagtg aaattttaat agacttaaat taatcgtggg tgtcgcacta agaatagagc
    13981 gacgcctttt tacatatttt acataagaaa taaagggctg tctcaaacta tagataagac
    14041 tctttttcat tgttgaaatg atgaaaatat atttgatttc atacaaaaaa gagtgcctca
    14101 taactaaaaa gttcactttg agacactcca tttttatatg ttagttgcat ttaaggtaaa
    14161 atagaagttt aatcatgttg aaataactga attacatctt ctaaatgatt gtacattgat
    14221 gtataaccta agtaagagtc tttactatca aatgccatca ttatcttgtt atggtggcta
    14281 atcatatcct taagagaatt tctatattta aaaccgaagt attggttaga agaaactgtt
    14341 ttaggtatta actcataata ttttataaaa attggattat gagaagctaa aattatctct
    14401 ttgtgaaatt gtaagtcagc atcataacat tcttctatca aaccatgaga aaccttatcc
    14461 aattcctcta ttgactttag gtaagtatat tttatttttt gaagttcttt agatgtagca
    14521 tttaaaattg ctagtttagt agcttcactt tcgataagtt gtcttgctct taaagattct
    14581 acataaaatt cttgtatgtt atgattttta tagatttcac catcatcaaa taggtcaact
    14641 tttttaaatc cttcaactat aggacttgag taaggcacag ctctaagtat tccttcctta
    14701 cagagtatat ttatggcatt tcttacaggt attctactta tgttaaatag gtcagcaatc
    14761 tttctctcac ttggtaattt actattaatt ttaaaagttc catttagaat gtttaattta
    14821 atataatcaa taattttttc agtggcattt aagcttttca taaaaactcc taaacatata
    14881 atttttttat gattgtatta tatcatagtt aagagttaaa tctacttttt tataatatgt
    14941 ataagacagg atagaggcca aaatagctat aatagccaag ataaagtatc catattgtat
    15001 tgaagtatat tccgatattt taccaactat cattggaaat acagacattc ctatagcata
    15061 aattgcttga aaaaaaccca ttgcagtaga tttctgatgt tttggtacat ttttcataga
    15121 ctcgctagta aggtaagaaa ggagtatacc tgtagatata cctggtagca cttgtagtac
    15181 aaatattata ttaatatttt ttattgttgg aactaaaaag caataggctg aaactaaaat
    15241 aaaaactaaa ggaatccaaa attttggccc atacttagta caaaatttag tagaagcaaa
    15301 gccagaagaa gcaacagcac acaccatgta agatatagaa gatagaccta tgagggtaga
    15361 actagaacct aagtttttaa gaatttgagt agtaaatgac attgttgtag acatttgaat
    15421 accctgttga attaatgcca taaaggaaaa aaatacaagt cttttatctt tgcagataga
    15481 taaatatttt ttaattgata ttttttcagg tatttttttt gaattatgat tttttatatt
    15541 taaggctaga atcgttccaa taactgaaga caatacactc atgatacata aggctgtcat
    15601 tcctaatttt tcataaaata aagtactcat aacaaatcca atcaacattc ctagattgtt
    15661 taccaatatt atcgtactcg tagctttttg ttgatgttct gtagaaaaaa agttggtata
    15721 gtgaaccatg taagaaatcc aagttgatga tgatattcca gataaaatgt tagctataag
    15781 aaaaccattg cctccaggag aaaagactct tataagagat gctaaaccag caaagaaaat
    15841 tccagacagt ataaatattt tatgtttatt tatagaatca gcaaatactc caacaggaat
    15901 ccttaataaa cattgtgaga ttccatatgc acctattatt ataccaatca tatttgtaga
    15961 tacattacat gcacttaaat atggattttg gaaaggtata tatacatatt gtgaaaacca
    16021 gaaaaatgta acaatagaaa gtaaaagtaa tttttgatta gatatttttt tcatattatc
    16081 ctccatatgg tatttttata atgcaaataa tataccattt agatttttta atcaagtact
    16141 ttctctatta aaaaaaattt aaatatataa ttattattta caaaaaaatt atactttata
    16201 gttgaattaa gaaaacgctt atgatactat aaagttaaca aatttgaata tataacagta
    16261 gacgtgttac tggtaatgca ggcaagatcg aatcaatttt tatagattat ctatagagta
    16321 ctatgggtat aatctattta tttggttcga tttttttgtt taattggcca taaaacaaaa
    16381 agcctatagt tgaaaatgga ggatttttta tggattataa aaatatagct caagaaattc
    16441 tactaaatgt aggaggaaaa gagaatgtaa atgaagtgac tcattgtatg acaagactta
    16501 gatttaaagt caaaagcgct tctaaagtga ataaggataa attatctaaa actgaaggag
    16561 taataactgt tgttgagagt atgggtcaaa tacaagttgt aattggaaat aaagtaaaaa
    16621 cagtatatga tgaggttata aaaattgtac ctcaatcaaa tgacgtaggt aaaaagaatg
    16681 aaagtcaaga aaagcaaagt ataattaatt ctatattatc agcagtagct ggtatattta
    16741 cacctacaat acctgctata gcaggggttg gtatgattaa aggtatttta tcagttcttg
    16801 ctatgtatta tatgaataaa aatggagtag atataaaaga aacgcaaagt tatattatct
    16861 taaatgccat ggccgattct atattctatt ttatgcctat tatactagga tatactgcgg
    16921 ctaaagtatt taatgcaaat aaaataatat ctatggtatt aggagctaca ctttgttatc
    16981 caacttttac tgctttgatg gcaggtgaag aatctgttag atttttagga ttggcagtaa
    17041 caaaagctac gtatacatca tctgttattc caataattat tgctatatgg gctctttcat
    17101 acgttgaaaa agtattagaa aagtatatac ctgaggtaat taaaataata atggttccaa
    17161 ctttatcatt ggttgtaatg ttacctgcaa cattattttt atttggacca atcggaattt
    17221 atataggaaa tgtcataaac ttttcttata agtatattta tgaattaagc ccagcgttgt
    17281 gtggagcatt tgtaggtggg ttatggtgtg tgttggttat atttggtgct catagagcat
    17341 tattaccaat aggtataagt gatgttgctc aaacaggaag acagaattta ttggcatttg
    17401 caggagctgc aaacttttca caagcagggg ctgctctagg agtattcttt aaaactaaaa
    17461 atcaaggact aaaaacaatt tctatgtctg caactataac agctttattt ggtataactg
    17521 aacctgcaat atacggtgcc aatcttagat taaaaaaacc tatggtatgt gccgtaatat
    17581 gtggtgcatt tggaggtgcg ataatgggaa tgggtggagc ttatggtaat gcatttgcaa
    17641 accaaggtgt tttaactata cctgtatatg ctgaagcagg tgcattaggt ttcttaagtt
    17701 atctaggtgg ttgtgctata gcattttttg gttcagctat ttcaacttat ctagttggat
    17761 ttgaagattt ggaagagtct tctaataagg aaaattcatt tataaaagta gaaacgaagg
    17821 atggtgttat tgatataact tcacctgtag agggcgaatg tatagaattg tcagaagtaa
    17881 aggatgatgt atttgcttca aaagctatgg gagaaggtat agctgtgtta cctacaaaag
    17941 gagtaataac agcccctact gattgtgaag tagcttcttt atttccaaca ttacatgcaa
    18001 taggattaaa attagataat ggagcagaaa tgttaataca tgttggaatc aatacagtag
    18061 agttaaatgg taaatacttt acaaaacatg ttaatcaagg agatttagtg aaaaaaggtg
    18121 ataaacttat atcgtttgat atagataaaa taaaaaaggc tggttatgat gtaactacgc
    18181 cagttatagt aaacaataca tttgattttg ggcaagtagt ttcttgtaaa tctagttatg
    18241 tttcaaccaa tgataatata atttcattag tattagctgg taattaagtt aatttgacta
    18301 gagataagta aattgattta aaaggaaata agtaaatcaa caattaaata catctaaaag
    18361 taagtttatt gcaatgaatt gttaaagaaa ataattaata tggagtaaag ctattgcaat
    18421 gaagtaacaa agaaaataat taatatagag taagtttatt gtaatgaatt gttaaagaaa
    18481 ataattaata tagggtaaat ctatctcaat gaaataacaa agaaaagagt taatatagag
    18541 tgaacttatt gcaatgaatc aacaaaaaaa ataatcaata taaaaaggag aaatatttat
    18601 gaatacagga tttccaaaag actttttatg gggagcatca tcttctgcat ttcaagtgga
    18661 aggagcttgg gataaagaca ataaaggaaa aaccgtagcc gactataatt cttttaaaaa
    18721 gtctcattta caggcagata ctaaagttgc atcagacttt taccataatt atgaagaaga
    18781 tattgaatta atgaaagaat tagggatgaa aacatatcgt ttttcaattt catgggcaag
    18841 gataatacca gatggtgaag gagagataaa ccagaaaggt ttagactttt ataataagat
    18901 aatagacaaa ctaatagagt gtgatattga gccattcgta actttatacc attttgacct
    18961 tccatttaaa ttagtagaga aatataatgg ttgggaaagt agagaaactg tctatgcttt
    19021 tgaaagattt gctaaaatat gctttaagca ttttggagat agggtaaagt attggcaacc
    19081 tcataatgaa cagaatttaa ttgttagagt ggaggagaga attaatatat atgatgaaac
    19141 agattcatgg aagatagata aaatgcgtgc tcagatggat tataatcttt gcttagcaca
    19201 tgcattagca gtcaatgcat gtcatgagat gataaaagaa agtaaaattg gagcagcagt
    19261 atcatcatct gtaacatatc ctcttacttc taagccagaa gatgtatatg cagctagaat
    19321 gaatgataat tttaaagtgt actatatgct tgatatgcat cattatggag aatatccagg
    19381 gtattatatg aagtatcttg aaaaaagaaa tacagttcct cacatggaag atggggataa
    19441 agagatactt aaaaaagcta aaatggattt tatagctgta aattattata ggacaaattg
    19501 tgcagaagct ttacctgaag attcacaaca tccatttgga ttaagagaag gaactgttga
    19561 ttttagtatg tatggactat ttaaaatgtc aatgaatcct aacttagagg cttctgagta
    19621 tggagcagca atagacccat caggtcttag agttgcctta aatgaatatt ggcaaagata
    19681 tcacttacct gttataataa cagaaaatgg tctaggagct aaagatatat tagaagatgg
    19741 aaaaattcat gatgactatc gtatagacta tttaagaagt catataaatg catgtaaact
    19801 agctattgaa gatggtgtag agatgattgg atattgtcca tggtcattta cagacctttt
    19861 aagttcttct caaggtttta ataagcgtta tggacttgtt tatataaata gaactgacca
    19921 tgaagtttta gatttaaaaa gaatcaagaa agatagtttt tactggtata aagaagttat
    19981 agagaacaat ggaatagtaa aataaacata aatatatatt gagggtttta gtatgtacat
    20041 tacaaaggta ttaaataatt cgttgctact tgcaaaggat gataatgatg aagaaataat
    20101 attgatgggg aaaggcattg gccataatta taaggccggg tctgagttaa ataaaaaaga
    20161 tatagaaaag atttttgttc ttcatgatga aaatataaaa aagagtatta tacaattggc
    20221 aacagaaata gatgcagaat attttggtat agctcaaatg ataattgcat atggaataga
    20281 aaaatataac ttaaaactta tgaaccatat atatttggcc ttaactgacc acattgcctt
    20341 tgcagtgaga cgctttaaag aaggtataat gattgaaaat cactatttgt tcgaaatcaa
    20401 ggaatttaat ccaaaagagt atgatatagg aaaatatgct attactgttt ttaaggaggt
    20461 tcttggttta gacctaccag aagaagagat tggtaatata gcaggccatt ttataaatgc
    20521 acaacaagat aatccttata gtgatagaaa taagagaagt gctaaaattg taaatgctat
    20581 tttgcaaata gttcattatc acttttctat tgtatataat aaagagtcat tttattatag
    20641 gagatttgta atgcatctaa aggccttttc acaaagattt ctggctaatg aaccatcaag
    20701 agaaaaaata gattttatat atgagcaagt tcaaaaaaat tgtaaggaag agtatgaatg
    20761 tgtgaaaaag gtaggagctt atatgtataa ggaatactcc agagaattac caagacaaga
    20821 ggagttatat ttgatgattc atattcataa gattttagga gaattatgcg aaaatagtta
    20881 gaatatctta atatgctagt aatattataa aaaattaaaa attaactaca ctttaaatat
    20941 gaacaattgc attacttaag ggggttgtct taaaattgat ttttttaatt aaatttgaag
    21001 ctcaactcct tttttaaacc tttatatcat tacaacttta atgtttatac gcctgtactt
    21061 tagatgtata tctacttttt acatttctca acatgcaaac ctaccatttt ttgcagtatg
    21121 ttaattgaaa taaaccattg agttaaccct aaatttattc tggacttata ggaatagttc
    21181 caaagatact ttaaaatatt ttctggaata attgcttatt tcacatatgt tttattgtga
    21241 tgttttgatt taataaattc ttcaactctg tatatagtaa atgtattatc atgtttgttt
    21301 tattcaagca cacttataat acgcttgatt ttttcacaat gatgaaatat actaatgggt
    21361 gttataggta taagaatcga attacacttg aagaagtaag tgataaatga gatataaatc
    21421 ctcatatgat aaactactat ttcattgagg aacatattct aagtgatgtc aagatactac
    21481 aatatgacta aattcctagt gataaaaaag tctacgaatg agtgtagaaa ggggaactaa
    21541 gatgaataaa gttttaatta tagatgatga taaagatctt tgtattttgt taaaaaagag
    21601 tatttcaatt gaacagattg aagcaaacta ttgccattct gggagagatg gactggttga
    21661 actttcaaaa caagattatc agttagtagt tctagacgtg atgatgccag gtatggatgg
    21721 ttttgaaact atggaacaaa tacgaaaata tagcagtatt cccatcttaa tgctgacatc
    21781 aaaaagcgat aattacagca aagtgcatgg attgcgagct ggtgcagatg actatctaac
    21841 caaaccattt gaaattacag aatttactgc tcgtgtactg tctttaatcc gacgttacac
    21901 ccgttttaat ccaaaaaatg aggaaccaca gcaactttct ttcaaaggtt tgttgattga
    21961 ccttgaccag catacagtga ctaccaatgg cgaacctatg ttgcttccac ccaaggaatt
    22021 tggtgtgctt ctttactgtg caaggcatca gggtaagatt ttgacaaaac agcagattta
    22081 tgaagctgtg tggggtgagc catatgtata tgatgacagc aatatcatgg ctattatcag
    22141 tcgcctaaga aaaaaaattg aactagaccc atcatctccc atctatttgc aaacaataaa
    22201 aggaattggg tatcaattca acacggaggt atgaaaatgg atgaacaaac gataattctt
    22261 ttcttattct tgctgtcgat tggtctactt tttctttcca tatttgcttt ttccaagctt
    22321 gggcatattc acaaacgcct aaaagatata gaggagatac tggctgatgt acaaattgga
    22381 aacgaaaatc gcaaaatact aattaagcca tgtgatgtta tggctccatt ggtttatcag
    22441 cttaatgaga ttgtctacga ttatgaaaat aagttacttt ctctgaaaaa gtcggataaa
    22501 gctagcaaac aacttatgac aagtctatct catgatgttc gtacaccatt aacaactctg
    22561 attggttatc tggatgctgt acacagtggt atagttatcg gacaagaacg ggaggaatac
    22621 cttgaaatcg ctaggcgtag agcctacgac ctcaaggatt acattgatgt tctttttgac
    22681 tggtttcggt tgaactctga tgaatttacg ctgtcaatag aatctgtgga aattgcagag
    22741 ttgtcacgca atattttgaa agactggatt cctatattcc atgaaaagaa actgaatttt
    22801 gaaattgata ttccagaaaa tcgactaatg gtaaatctcg acccagatgg atattcccgt
    22861 gtggttaata atctggttca aaatgttctt gctcacagta aggctcggca aatcaagatt
    22921 accatgagtg aggattccag aatggtatta ctccgtgttg atgatgatgg tgttgggatt
    22981 gccagagaaa atttaccaca tatttttgag cgactgtata agtgtgataa aggacgttca
    23041 gaaaaaggaa gtggtcttgg tctatctatt gtcagtcaaa tggtggagcg aatgggtggt
    23101 cagatttccg tagaaagtga gattggaaag tataccgtat ttacagtgag cttaccatta
    23161 atataaaatt gtttttcccc catgtgacat gtatacacag ggggattttt tgtaagttat
    23221 aaagttgcaa ggttaataca aggttgttgc aaggttaatg taagattgag gtggtatgat
    23281 agctttaata aacaaaggag gattttatta tttatgaata atttaattat tgaaacaaaa
    23341 aatctcacta agcaatatgg tgctcaaaag agcgtttcta atttgaatat tcacgttcag
    23401 aaaggtcgca tttacggatt gctaggaaga aacggtgcgg gtaagacgac aactatgaaa
    23461 atgttattgg gtctaacaaa accaactact ggagaaatta aaatatttgg tcaggacatt
    23521 cacaataacg agaagaagct tcttcctcga attggatgtt tgattgaatc accagggttt
    23581 tatcctaatc tgacaggaac agaaaatctc aaaatctttg caaggcttag aggaatacca
    23641 aaaaaaaatg cagtagaatc agcactggat gtagtcggtt tgccaaacaa tgacaaaaag
    23701 ttattttctc aatactctct tggtatgaaa cagaggttgg cgattgctct tgccattatg
    23761 catgacccac aactgttgat tttggacgag cctatcaatg gacttgatcc tattggtatt
    23821 gcagaagtaa gaaagtttat tcgtgatctc tgcacagaac gaggtaaaac tattttgatt
    23881 tccagtcaca ttctttctga aattgccttg ttggcagatg acattggtat tattgaccat
    23941 ggtattttgt tggaggaaga aagtcttgcc caattagaag aaaaaaacag taaatacata
    24001 cattttgctg tatctgatgc agcacatgca gcaaggcttc tggaaaccca acttaacctg
    24061 aaaaatttca aagtagcaga taattacaat attatgctat atgatacaga tgtttctgtt
    24121 gcagaaataa atcgtgcttt cattctaaac aacattgagg tatcagaatc tcatttatgc
    24181 gaagatactc ttgaagatta cttcaagaaa gtaacagggg gtgaaggaat tgcttgatat
    24241 aatttcatgt gaattttcaa aactcaagcg gttaaaattt atactgattt ctattttagg
    24301 tgcttgctta tttccaattc ccgcaacaat tttgattgca aaggacaact tgccctttga
    24361 gcaattgttc aaattagttg tcaattttgg atattttttg ctattaccaa ttgtattaag
    24421 tattgttgct agccaactat tttttataga aagagataac gatgtattaa aaaatcttgc
    24481 aacagtgcca gtgccaaaag gaaaactggc tctggcaaaa ttggctgtgt tgctgtttat
    24541 atcattgttc tactccgttg cagggttagg agctactatc attggaggtt tcattgttgg
    24601 aatagtagag ggggttactg taaaattagg aatgagcatt gcttttggaa ttatgttttt
    24661 tgttactgta cttcctgttg tggtactcat tgtatatttc aatcgcagct atattttttc
    24721 tattatactt tcttttgtat atgctatttt caattttagt atatccttaa atattatcaa
    24781 ttttgaacca aataacccac tattaagcgt attacctgct cctgttataa tgcgttggtg
    24841 gatggcattt tggggagatc ccactggcga atacacggca cttagacagc catatttgct
    24901 ctcaactcct gcttgtgtag gtattttatg tttgataacc attatagcag tcctgctgat
    24961 ttgtatgata tacaaaaagc aggagaatta ggaggtatct atgcttgata taattactac
    25021 agaatttcaa aaaataaaaa gatataatat tctatggatt ggtgttgttg ctgttctgtt
    25081 ttcagcacta ttggcagttt ttcaggttgt aagttcacac ggcagtgacc ctctaacgta
    25141 tgagagcttt gcaaacggag ttatctggaa taatttcagt cttagttttc catttgggat
    25201 tacacttatt ggaggatatc ttattaaccg tgaatataca gaccaaacct taaaaaacat
    25261 cctaactgtt cctatttcat tacgcaaatt gctagtggga aaattaattg cggttggagg
    25321 aattgctgta ttgttaagtc ttttcagctt cttatgcaca tttattttgg ggttgttatt
    25381 ttgcaatatc aatatatctg ttacactcat tgtaaaatcc ttagttcaga ttttgtctgt
    25441 taatctgtat tgttatgttg ctgttttgcc gattattgct tattttggtc gtaaacaaaa
    25501 tgcatttctg acgggtgtag ggattgcatt tgtatacggg ttttgtggtg tgtttgttgc
    25561 tggaagaaat ctgacagact tttatccaat cacagcaggt cttggtctag ttggatacaa
    25621 caatggtgct ggtactgttt atcagccact tatcggtgct atgactttga ttgtaattct
    25681 aatcttgaca actattctac ttgtatttac acctaattat gataaagtta tgaccatatc
    25741 taagaaaaaa gggataaagc ataaaaagta ttctcataaa taacataaat attgtttcta
    25801 ttatttaagt tgctagacag tgaaaagaaa aaatctgtcg tacagtggct atatgctcat
    25861 gtattcaaga agctacagtt ttgatttttc ataactgtag cttctttttg tatttattaa
    25921 atcacggcta tcataatact tgcaataggt atagcgacaa gagagagtac tgtcatgtgt
    25981 gttttatata ttacattaag tatattctaa aatatatgtt ttaaataata tatttgaaaa
    26041 taggaatatt taaataaaaa aaataatatt atatatagtt gacaaaaatt ggcaaatgat
    26101 ataatatata aatatagaaa tagtaatcat tactattatt aaaaagggag gaattaatta
    26161 tgaacttaaa aggaactaaa acagaaaaga atttaatggc aggatttgca ggagagtcag
    26221 aagcaagaaa taaatataca tactacgctt caaaagcaag aaaagaaggt tataatcaaa
    26281 tagctgctat atttgaagaa actgctaaca atgaaaagga acatgctaaa ttatggttta
    26341 aacttttaca tgatggtatg ccttcaacag aggaaaattt aaaggatgca gcagcaggtg
    26401 aaaactatga atggactgat atgtatgcaa aatttgctaa agaagctaga gaagaaggat
    26461 ttgacaagat agcttattta tttgaagctg ttggaaaaat agagaaagag catgaagaaa
    26521 gatacttaaa gcttttagaa aacttaaatg aaggaaaaat atttaaaaga gatgaagaag
    26581 ttgtttggca atgtcaaaac tgtggtcatg tgtatgtagg tactgaagca ccagaaaaat
    26641 gcccagtatg tgaccatcca aaagcgtatt tcaatataaa agctgaaaat tattaaaaat
    26701 tatcattatt aaaaaggaat gttaactatg aaattttcta aacaacgaga actgatttta
    26761 aatgaaatat taaataatcc ggttcatcct actgcggatt acctatatga aaacttaaaa
    26821 aaagataatc caaatttaag cttaggaact gtttatagaa atttagctca gctaacggaa
    26881 catggcttta taagaaaggt tagtattcca gggtatccag atagatttga tggtcgaata
    26941 gataatcact atcatatcat atgtgaagta tgtggagagg tatacgattt agaatccgaa
    27001 gttcttaata atttacaaga attaatatct gaggagacag acataaaaat aacatcttat
    27061 aacataagtt ttaaagggat ttgtaataat tgtaaaaggt gtagccaagt aggttagttt
    27121 tcaaattcta aaaataatta atgaggtgag tttatgtgta gtgaacaaaa attttttata
    27181 tgtgagactt gtggaaacct tgtaggaatg atacaaagtg gtggtgtacc tattttctgt
    27241 tgtggaaaac ctatgaagga acttgttcct aatacaacag atgcagcagt tgaaaaacat
    27301 gtacctgtaa ttgaagtgga tggaaataat gttactgtta aagtaagtag cacaactcat
    27361 ccaatgacta aagaacatca tatagcatgg gtgtatctta tgactgaaca aggtagtcaa
    27421 cgcaaatgtc tagcagtaga tggagaacct gtagttaaat ttgctttgaa tgatgatgat
    27481 aaagtgattt ctgcctatgc ttactgtaat ttacatggat tatggaaggc tgaactgtaa
    27541 tatttaaata aattgagtca aagaggaggc tataaatgta ttttcataca aatagcctcc
    27601 tataaattta tataaattca agttttaatt ttgtttaatg tcaatataat ataaggagaa
    27661 aataatatgt caatttataa atgtagtgtt tgtggatata tttatgatga atctaagaat
    27721 gataaaacat gggatgaatt aagtgaagat tgggaatgcc ctgtatgtac aaaaggtcgt
    27781 agttattttg gaaagataag cactgtttac tatgaagaag atgaaaagat agcagaagac
    27841 atagttgaaa atgaatctaa attaaatact gaaaaagagg gagatttaaa ttacttaagt
    27901 acatatttaa gaagagatga tgaagttgag aaacatatgg atataattca cgaaatggct
    27961 gttacaggaa aatctataat agaacctatg agaactaaac taccagtaat ttcttgggat
    28021 gatattttaa ttatgggtgc tcaattaaat ccattacctt taaatgagca tgatgaagta
    28081 aatacaacaa caataatagg aaaaaaagca aagaaaccta tgattataga aaatccagtt
    28141 tatatttccc acatgtcatt tggagctttg tctaaagaac taaaaatagc tttagcaaag
    28201 ggtgcagccc aaaataagac agctatgtgt agtggtgaag gaggtatatt accagaagag
    28261 aaggaagctt catacaagta tatttttgag tatgtaccaa ataaatatag tgtaactgaa
    28321 gaaaatctta aaaattctga tgctatagag ataaaaattg gtcaaggaac aaaacctggt
    28381 atgggaggtc atcttcctgg cgaaaaagta actgaagaaa tagcaaaagt aagaaataag
    28441 cctgtaggtc aagatgtaat aagtccatct tgttttgagg aaatacaatc caaagaggat
    28501 ttaaagaaac ttatagatga acttagagaa gtttcagaag ggcgtccaat aggtgttaaa
    28561 atttcagcag gtcatattga aaaagatatg gaatttatag cttatgctaa acctgatttt
    28621 gtaactattg atggtcgtgg tggagctact ggagcaagtc caaagttgct taaagatgca
    28681 acttcaattc caacaatatt tgctctttat cgtgctagaa aatacataga tacacatgga
    28741 ttagatatag atttggttat aacaggtggg cttcgtatat ctactgattt tgctaaggca
    28801 attgctatgg gggctgatgc agttgctatt gcaagttctg ctcttatggc agcggcgtgt
    28861 caacaatatc gtatatgtgg ttctggtaag tgtcctgtag gagtagctac tcaggatgag
    28921 gaattgagaa aaagattaca tatagaaaat agtgctaata gagtggcaaa ctttttaaat
    28981 gtaagtttag aagaacttaa gacttttgct agaatttctg gtcataaaga tatacatgat
    29041 ttaagtgttg atgatttata tactgttaac tctgaaatat caaattatac aaacatacag
    29101 catgtataga ttgaagtttg aaactattag attttagata gctataaaag taaatgataa
    29161 atatatttta taaaagagag tgtcttaaaa tgaacttttt agttcataag tcactctttt
    29221 ttgtatgaag ccaaatatat ttatcatttt agcaatgaaa aagaatctta tctctttatt
    29281 ttgagataga ctccttctat ttatgaaaat taaagatatt aaattgtaaa atattctgtg
    29341 atgtcgtact attaaactaa agattctgta ctttaaagta aataaaaatt taaggagtgc
    29401 ttattatgga aagattgaag atacaagttt tagttgacaa taatacttat attgatagat
    29461 actttgttgg agaaccagca gtaagctatt atattgagat tgatggaaat aggattttgt
    29521 ttgattcggg atattcagat gtattcatat caaacgctga aaaattaaat atagatttag
    29581 gaaatttaac tcatgttgtt ttttctcatg gacataatga ccatacaaga ggtatccagt
    29641 ttttagaaaa taggtatgat ttatctacag ttgaacttat ttcacatcct aactgtttta
    29701 tacctagaaa acatggagag aaaagcatag gagctccatt ttctgctgag gaaattaaaa
    29761 atatttttat gtataatcct aaggataagc catttaatct aagtaagaat tgtgtgtttt
    29821 taggtgaaat accttctata aataattttg aaaaaagagc taaaattggt aaatgtaaaa
    29881 taggagattt gtgggaagat gattatgttt tagatgattc tgctatagtt tgtaaaactg
    29941 ataaaggtat ttttattgtt acaggatgtt ctcatagtgg aatttgtaat atagtagagt
    30001 atgctaaaag ggtatgtgga gatgatagag ttattggtat actaggtggt tttcatcttt
    30061 ttgaattgaa taataggtta gatagtacaa tacaatattt ggataaagaa gaaataagaa
    30121 tgttgtaccc ttgtcactgt gtttctttta aggtaaaagc taagatgaat gaaatattga
    30181 atataaatga agttggagta gggctgacca taagtatata gtatttgaaa gtatataaga
    30241 tttaagttga aggttaaaaa gatattttct atataaagta tcttttttgt tacgtttatt
    30301 ttaagagaaa tcaatatact tttatatgaa atattgtaaa aaaatttatt tttaatgaga
    30361 atatattttc attaataaac aattttatta tgtattaata atgctagaat aaatataaat
    30421 aaatcaaagt tacttacatt aaagtatatt ttttcacaaa atatattgat ttattctttt
    30481 aaagtttgat aattcc
//
LOCUS       DACTGW010000022        30217 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.022, whole genome shotgun sequence.
ACCESSION   DACTGW010000022 DACTGW010000000
VERSION     DACTGW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.022"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(204..809)
                     /locus_tag="I9573_000791"
     CDS             complement(204..809)
                     /locus_tag="I9573_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAT4795425.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(934..1051)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1111..2277)
                     /locus_tag="I9573_000792"
     CDS             complement(1111..2277)
                     /locus_tag="I9573_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4795426.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(2425..4566)
                     /locus_tag="I9573_000793"
     CDS             complement(2425..4566)
                     /locus_tag="I9573_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAT4795427.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYNEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(4710..5726)
                     /locus_tag="I9573_000794"
     CDS             complement(4710..5726)
                     /locus_tag="I9573_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAT4795428.1"
                     /translation="MKNIQINEEKMNQQDFWNDNEVAQRVLQENKSLKETLEEYESLK
                     SLLEDIEVLIEIGLEEDDDSVERDIEKSIESMEEKLSEMKIKTLLNGEYDKNNAILSI
                     NAGTGGLDAQDWAQMLLRMYIRWSESKGYKVKLLDIISDPEAGIKTATILVEGTNAYG
                     YLKSEKGVHRLVRISPFDPSGKRHTSFASIDVTPELDENIEVEINPSDLKIDTYRASG
                     AGGQHVNTTDSAVRITHIPTGVVVQCQNERSQHLNKDRAMRLLMAKLIELKELEQKEK
                     IEDIQGKYSQITWGSQIRSYVFQPYKLVKDHRTNAEFGNVDSVMNGNIDLFINEYLKM
                     NKIV"
     gene            complement(5832..8507)
                     /gene="secA"
                     /locus_tag="I9573_000795"
     CDS             complement(5832..8507)
                     /gene="secA"
                     /locus_tag="I9573_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAT4795429.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMVREDLPDSVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(8639..9190)
                     /gene="raiA"
                     /locus_tag="I9573_000796"
     CDS             complement(8639..9190)
                     /gene="raiA"
                     /locus_tag="I9573_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAT4795430.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDSEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(9743..10417)
                     /locus_tag="I9573_000797"
     CDS             complement(9743..10417)
                     /locus_tag="I9573_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAT4795431.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(10420..11472)
                     /locus_tag="I9573_000798"
     CDS             complement(10420..11472)
                     /locus_tag="I9573_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAT4795432.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISKKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKTPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSNTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(11520..12479)
                     /locus_tag="I9573_000799"
     CDS             complement(11520..12479)
                     /locus_tag="I9573_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HAT4795433.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIVDFTFSVV
                     TKEESPAVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(12575..13093)
                     /locus_tag="I9573_000800"
     CDS             complement(12575..13093)
                     /locus_tag="I9573_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HAT4795434.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNEKEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            complement(13104..14438)
                     /gene="celB"
                     /locus_tag="I9573_000801"
     CDS             complement(13104..14438)
                     /gene="celB"
                     /locus_tag="I9573_000801"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAT4795435.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIVTPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(14477..14803)
                     /locus_tag="I9573_000802"
     CDS             complement(14477..14803)
                     /locus_tag="I9573_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4795436.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(14828..15136)
                     /locus_tag="I9573_000803"
     CDS             complement(14828..15136)
                     /locus_tag="I9573_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAT4795437.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(15277..17181)
                     /locus_tag="I9573_000804"
     CDS             complement(15277..17181)
                     /locus_tag="I9573_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT4795438.1"
                     /translation="MNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKYIDE
                     WLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLLLCN
                     NKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIEGDEKDIRIM
                     LVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLMLFLL
                     INIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLILGMN
                     KQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTMHQM
                     KSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLERKY
                     SKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTIDFII
                     AFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIRELE
                     HDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIKQSV
                     LCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDILSKC
                     DIEKVKDILI"
     gene            complement(17522..18304)
                     /locus_tag="I9573_000805"
     CDS             complement(17522..18304)
                     /locus_tag="I9573_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAT4795439.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(18307..20526)
                     /locus_tag="I9573_000806"
     CDS             complement(18307..20526)
                     /locus_tag="I9573_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAT4795440.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(20790..21887)
                     /locus_tag="I9573_000807"
     CDS             complement(20790..21887)
                     /locus_tag="I9573_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795441.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSNIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(22203..23396)
                     /locus_tag="I9573_000808"
     CDS             complement(22203..23396)
                     /locus_tag="I9573_000808"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAT4795442.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(23533..23636)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            23825..24334
                     /gene="yyaC"
                     /locus_tag="I9573_000809"
     CDS             23825..24334
                     /gene="yyaC"
                     /locus_tag="I9573_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HAT4795443.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(24445..24873)
                     /gene="fabZ"
                     /locus_tag="I9573_000810"
     CDS             complement(24445..24873)
                     /gene="fabZ"
                     /locus_tag="I9573_000810"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAT4795444.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEIEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(24889..25908)
                     /locus_tag="I9573_000811"
     CDS             complement(24889..25908)
                     /locus_tag="I9573_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAT4795445.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(26105..26365)
                     /gene="spoIIID"
                     /locus_tag="I9573_000812"
     CDS             complement(26105..26365)
                     /gene="spoIIID"
                     /locus_tag="I9573_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HAT4795446.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(26463..27131)
                     /locus_tag="I9573_000813"
     CDS             complement(26463..27131)
                     /locus_tag="I9573_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HAT4795447.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(27197..28261)
                     /gene="spoIID"
                     /locus_tag="I9573_000814"
     CDS             complement(27197..28261)
                     /gene="spoIID"
                     /locus_tag="I9573_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HAT4795448.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKSTVSYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(28348..29601)
                     /gene="murA"
                     /locus_tag="I9573_000815"
     CDS             complement(28348..29601)
                     /gene="murA"
                     /locus_tag="I9573_000815"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAT4795449.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(29622..>30217)
                     /locus_tag="I9573_000816"
     CDS             complement(29622..>30217)
                     /locus_tag="I9573_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HAT4795450.1"
                     /translation="NVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGD
                     KIQLCATIKGSDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSS
                     QVDVSACIVGEYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDY
                     ELEYLGEKINLNIGIKYNEDDGKTLIYIATPIIKLDY"
BASE COUNT         8997 a         5689 c         3182 g        12349 t
ORIGIN      
        1 tttttcatat tttaatagtt aatcgtttta attcttcctt tattttaaaa tttaaatttg
       61 tctaaatatt tcttatttta tatacatttt ttgataaatt aataaagcca taatatattt
      121 aatatatcaa tcacaatata ggactgaagt tataaataaa ttatggctat tcaatacatt
      181 taattttata ttttcttgtc taattatttc tttattatat ttcccatttt tttataaagt
      241 ggcaatgtta ctaaagatat tataacagcc ttcagcacat taaatggagc tatcatccaa
      301 attacaaaag tgaataaatc atgaacttga ggatttatag ctgaacctaa acctattata
      361 gcatctagtc ccataagttg accataaagt ggcaacatta caaaataatt tactaatgaa
      421 ccaattatag tcatagatat tgttcctaat acaaaaccaa tcagtacacc ttttaaatct
      481 ccacttctct tatatgaata acaaagtatc attacataag aaccaccaat taatatattt
      541 gcaaattctc ctattcctcc agttgttgtt gctgttattg cttgtaacac attttttact
      601 acaactattc caaatccaac taatggtcct agagacattc ctccgaatat tgctggaata
      661 tctgataagt caatttttaa gaaatctgga aatatacctg gtattggtgc ttgaataaac
      721 attaataaat aagatattgc agataaaatt gacattacta ctaaagttct tgttgaaatc
      781 atattgcctc tttttgctgt gttttgcata attattttcc ccctaatcaa tatgtttata
      841 ttttttagga aaaacaaaaa actcgaagac cctatcttcg agtttttatg gatatagttt
      901 aaacaaacta aaataaactt atttatcttc cataaatatc ttctctcatc cagactttac
      961 tgtcggcttt ggaatctcac caaatcaacc acctaagtag ttcgcgggct ataccgccgg
     1021 tagggaattt caccctgccc cgaagatctt ctttgatttt ttaattttcc ttacactctt
     1081 atattagtta attttttaat aattgtcaag ttattttttc tcatacacta ctttgccatt
     1141 tattatagtg taaagtacat tactttgaat ctctagtgga gaattatccc aaattactag
     1201 gtctgcatct ttaccaactt ctatagagcc tactctatct tctattccta aagtcttagc
     1261 tggatttatc gtaattgatt ctattgcttt ttcttctttc ataccatgtt ttactgcaat
     1321 acctgcacat attggaagat attgaactgg tattactgga tggtctgtca ttatacatac
     1381 atcaagacct gcatttgaaa gtattcctgc tgtattaaat gtcaaatttc taagctcaaa
     1441 tttagacctt tctgacaaag atggccctac tattacagga aacccttctt ccactaattc
     1501 atctacaata agatgacctt ctgtacaatg gtctaatgta agctttaaat taaattcttt
     1561 tgcaattctt attgctgtaa acatatcatc tgctctatga gcatgtgcct taaaaggaat
     1621 ttctcttctt aaaactggta ttaaactttc catttttata tcatattcag gtttttcaca
     1681 gtcttcatca tcatgactct catacatatc aatatcctct aaatattctt ctgctttttt
     1741 taggttttct ctaagtaaag cagctatagc cattctagtt tgaggagatt tatcatcctg
     1801 accataacag ctctttggat tttctccaaa agcaatttta gatgcaactg gattttttat
     1861 taccatttta tcaattcttt ttccacatgt ctttatagct atacattgac cacccataac
     1921 atttgcactt ccaggggttg tacaaacaga agttattcca ccttcaaaag cttctttaaa
     1981 agttctatcc attggattaa ttccatctat aggatttaat tgtggtgtta ttgggtcagt
     2041 ctcttcattt ccatctgctc cttcaaaccc tataccatct tcccataaac ctaagtgagt
     2101 atgagcatct ataaatccag gaaacacaag tttattacta gcatctatta cctctacatc
     2161 taaaggagct atcaaatctt ctccaatttc tattattttt ccttcatcta taagtatatc
     2221 tccatgaatt atcccatttg ttatggtatt tattttacca ttttttataa aaatcattca
     2281 ttttcctcct gattttcaag actatcgttc ttattttaat atattttctt actatatact
     2341 tttacaaatt tttaacattt atacactcga gtaatctcat ttacaaatat agaatttaag
     2401 tactataacc atacataaac ttatctattt tttcattgat aaggcaactc tttttttgtt
     2461 taagtcaatt ccaatgactt ttacatctac tatatcacct actgtaacaa tactcatagg
     2521 gtctttcaca aaactattac tcatttctga tttatgaact aatccgtcat tttttattcc
     2581 aatatctaca aatgcaccaa agtctacaac atttcttatt gtacctttta aagtcatacc
     2641 ctcttgtata tcttcaattt ttaagacatc agttctaagt ataggtttta taccttcttc
     2701 tcttgggtct cttcctggtt ttttgatttc agctatgata tcttttaacg taacttgtcc
     2761 tacttccagt ttttcactta attccctcaa gccaatttct tttatttttt catctatttc
     2821 acctatattc ttattcttta catcatctaa agaatatcca attatctcaa tcattttttt
     2881 acatatatca taactttcag gatgcacacc tgtattatct agtggatttt taccctctaa
     2941 gattctcata aatccagcac attgagtaaa tgcttgaggt cctagtcttt ttactttttt
     3001 caattgtgct cttgatgtaa aatctccatt ttcttctcta tatgctatta tattttttgc
     3061 tatagcttta cttattcctg caacatgttc tagtagggag tatgatgcgg tatttaaatc
     3121 tactccaaca ctatttacag aatcttcaac aactccatct agcacttctt ctaatctctt
     3181 tttgtttaaa tcatgctgat attgaccaac accaatactt ttagggtcta tttttacaag
     3241 ctctgctaat gggtcttgta atcttctagc tatagatata gctcctctta ttgatacatt
     3301 tatatctgga tgttcctcat ttgcaagttc agatgctgaa taaacagatg ctcctgcttc
     3361 actaactatc acatactgca cttcgctatc tatttctttt atcatttctg atacaaaagt
     3421 ttctgattct ctagatgcag taccatttcc aattgaaatt atatctatat cgtatttttc
     3481 aatcaatcct tttaaaactt tctttgctcc ctctacatca ttttgagggt ctgttggata
     3541 tactgttgta taatctagta gtttaccatt tttatctact acagctattt tacatccagt
     3601 tctaaatgca gggtcaaatc ccataactac tttgtcttta actggtggtt gaagcaataa
     3661 acttttaaca ttctttccaa atacacttat agctctttct tgagctattt ctgttaaatg
     3721 atttcttatt tctctttcta ttgatgggaa aattaatctt ttataagaat cttctattga
     3781 acttacgatt tcttctttat ttttgaaatt tttatcattt acatattcgt ttattatgta
     3841 atttaaaact ttatcattat ttatttctaa cttaaccttt aggaagcttt ctttttctcc
     3901 tctgtttatt gccaaaactc tatgtggagc catacttttt actgcttcgt tataatcata
     3961 atacatatca taaaccgatt tctcatcagt agcacttttg ctaactatca taccttctct
     4021 taaagctaat tctcttatat actttctaat cttagcatca tctgagacta gttctgcaat
     4081 tatatctcta gcacctttta gagcatcttc aatagaaaga acttctttct cttcatcaag
     4141 ataattcttt gcctctattt ctatattatc taaattattc tctagtattg acaatgctaa
     4201 attttctaat cctttttctt ttgctattgt tgctctagtt cttttctttt gtttatatgg
     4261 agcatatata tcttctactt cttgtaatgt ttttgctttt tctatatttt gtgttatttc
     4321 atctgttaat ttcccttgtt catctattag tctaactaca tcatcttttc tactttgaag
     4381 atttctcaaa tacattaatt tttcataaaa ttctcttagt gtaacatcac tcatttcgcc
     4441 agtcatttct tttctatatc tagctataaa tggtattgta tttccttcat caataagttt
     4501 taatgtatta tttatttgtt catctctaag attaaactct ttttttaaga tttgatttat
     4561 atccaattta ttcactccta atgttagttt ttcataagta taacaaaaca gaacgaaggc
     4621 tgagggttat cgtcctcagc ctaagtgctg taagtgcata ttaagcacta accttcttat
     4681 acatatatct taattacagt gcatatttat taaactattt tattcatttt taagtattca
     4741 ttaataaaca agtctatatt tccattcatt acactatcta catttccaaa ctcagcattt
     4801 gttctatggt ccttaaccaa tttatatggt tgaaaaacat atgacctaat ttgactaccc
     4861 caagttattt gtgagtattt gccttgtata tcttctattt tttccttttg ctctaactct
     4921 tttaattcta tcaatttcgc cattaaaagt ctcatagctc tgtccttatt tagatgctga
     4981 gacctttcat tttggcactg aactactaca ccagtaggga tatgtgttat tctaactgct
     5041 gaatcagtag tatttacatg ttgccctcca gctccagaag ctctatatgt gtctatcttt
     5101 aaatctgacg gattaatttc tacttctata ttttcatcta actctggtgt tacatctata
     5161 gatgcaaatg aagtatgtct tttccctgat gggtcaaatg gagatattct cactaatcta
     5221 tgaactcctt tttcactctt taaataccca taagcatttg ttccttctac aagaattgta
     5281 gctgtcttta tccctgcttc agggtctgat attatatcta aaagcttaac tttataaccc
     5341 ttactttctg accatcttat atacattctc aaaagcattt gagcccaatc ttgagcatct
     5401 aagccaccag taccagcatt tatagataat atagcattat ttttatcata ctctccattt
     5461 agaagagtct ttattttcat ttcagataat ttttcttcca ttgattctat ggacttttct
     5521 atatctcttt ctacagaatc atcatcttct tctaaaccta tttcaattaa tacttctata
     5581 tcttctaata agctttttaa gctttcatat tcttctaatg tctcttttaa agatttattt
     5641 tcttgaagta ctctttgagc gacttcatta tcattccaaa agtcttgttg attcatcttt
     5701 tcttcattaa tttgtatatt tttcatcaat gaagctatgt caaagagaaa ccctcaattc
     5761 ttctaaattt gaagccatct gctccatact atgtctatac tcttgtaact ttaacattta
     5821 atcctccgag attatcttcc acaacagttt ttatattttt taccacttcc acaagggcat
     5881 aagtcatttc ttccaacctt ttcatctttt ttaacagttt tacttgttgg aagtgttcca
     5941 tctgatggag aagatagatt ttcaacatct acaactgctt ttctttccac tggagtttct
     6001 attgttatat taaataaata tcttacagtg tcttctttga tatgtttatt catttcatca
     6061 aacatatcaa aaccttccat tttataagca ataactgggt cttgctgacc tacagcacga
     6121 agaccaatac cttggcgtaa ttggtccata gcatctatat ggtctatcca atggttatca
     6181 acagcttgaa gtaagattac tctctcaact tctctcattc tctcatagcc aacttgttct
     6241 tcttttgaag tatatatctt catagctatt tcataaactt tttctgttat ttcaactgga
     6301 ttaagttttt ctatttctgg tatttctatg ctaccttttg gcaagaacag attatacata
     6361 tgttctttga atccttcttc atcaaatcct ttatcttgtg tataaagagt aactgcttct
     6421 tctattatag aatgagtcat tgattgtatt tgttcttgta aatcttcacc ttctaataca
     6481 cgttttctct ctgcatatat tatttctctt tgtttattca taacatcatc atattgaagt
     6541 acatgctttc ttattccaaa gtttttacct tcgactttct tttgtgctcc ttcaatagac
     6601 ttagataata ttctatgttc tatcggcatg tcttcttcaa gacctatttt atctacaata
     6661 ccagatattc tatcactacc aaacaatctc ataaggtcat catcaagacc tatatagaat
     6721 cttgaactcc ctgggtcacc ttgacgacca gcacgacctc tcaactggtt atctattctt
     6781 cgagattcgt gtctttctgt acctattata gcaagtccac ctgcttctac aacttgtttt
     6841 tgttcttctt gtgtttgttg cttaaacttt tcaaatagtt tttcatactc ttctctagct
     6901 tcaaatagaa tttcattacc ttttacaggt ataccttcta ttggagtatc taccctattt
     6961 actatttcct ctttaaatcc atttcttctc atttctctct tagttaagaa atctgggttt
     7021 ccacctaaaa ctatatctgt accacggcca gccatatttg tagctattgt aacagcgcct
     7081 aatctaccag cctgtgctat tatctctgcc tctttatcat gatgcttagc atttaatact
     7141 tcatgtttaa tgcctttttt ctttaatatt tgtgataata attctgattt ttctatcgat
     7201 actgtaccta caagtattgg ctgattaact ttatgtcttt caattatttc ttgtgctaca
     7261 gcattaaatt tacctatttc acttttgtat acagaatctg gtaaatcttc tcttaccata
     7321 agcttattgg taggtacttg gaaaacgtcc attttataaa ttgctttaaa ctcttcttct
     7381 tctgtttttg ctgtaccagt cataccagaa agttttttat acattctaaa gtagttttgg
     7441 aatgttacag tagctagtgt tttagattct ctttgtattt taagaccttc tttagcctct
     7501 attgcttgat gtaaaccttc tgaatatctt ctaccaaaca taagtctacc tgtaaactca
     7561 tctactatta ctatttctcc atctttagca acataatcaa catctttctt cattattaca
     7621 tgtgctctta aagcttgatt tatatgatgg tataattctg tatgagatat atctgttata
     7681 ttatcaacat taaaatacac ttctgctttt tgtatacccg atgctgttag agatacagcc
     7741 ttatcttttt cttctaattc ataatcatct ggttttaagg ttaaaacaaa tgtgtttgca
     7801 tctgaatata agtgagttga tttgtctcca ggtccagata tgataagtgg tgttctagcc
     7861 tcatctatta gtatagagtc aacctcatcc actatagcat aatttaatcc tctttgaact
     7921 ctttgctctt tgtgtattac catattgtcc tttagatagt caaatccata ttcattattt
     7981 gtaccataag ttatatcaca atcatattgt tcttttctaa cttttggatt ttgaccgtgt
     8041 ataataacgc ctacactcat tcctaaaaac tcatagattt ttgccatttg gtctctatca
     8101 cgttttgcaa ggtaatcatt tactgttaca acatgtacac cttttccagt aagcgcattc
     8161 aaataaacag gagcagttgc aaccaaagtt ttaccttcac ctgttttcat ttctgctatt
     8221 cttccttgat gtagtactat acctccaatc atttgaactc tgtagtgtct aagtcctaac
     8281 actctcttag aaacttctct aactactgca aaagcttctg gaagtatatc atctatactt
     8341 tcaccatttg ctaatctttc tttaaatata tttgtcatat ttttaagttc cgagtctgcc
     8401 atagattcaa atttaggttc taatgaatct attatatcaa cagttttatt gaactttttt
     8461 aattcttttt tatctgccat gttgaataaa ttatccataa agctcatatt ttacatctct
     8521 cctcgttctt tattgtacta ttattcattt taccataaaa actatacttt taatattaat
     8581 ttaaaaagct atcgataatt tatttatcga tagctaatat gattaaatct aaattgcttt
     8641 attcatgttc tattatacca tatccaccat tattacgctt ataaactact gctatttcat
     8701 ctgaatctat gttcttgaac atataaaaat catgtcctat taattccatc tgtaatacag
     8761 cttcttcttc actcattggc ttaacactaa atttcttacg tctttgaatt actaattctt
     8821 gattttcatc cccataatcc tcttgttcat cagctagatt tatctctgaa tcttggaatc
     8881 ttatactctt gttgtcttta tgtttatctt gtaatctctt tttatatttt tttaattgtt
     8941 tacttaattt atcatataca acatctatag ctgtgtataa attttcctgc atatcttctg
     9001 ctctaattat aggaccattt acagtagcta ttgttacttc tattttttgt cttgcttttc
     9061 tcgcacttac tgtaactttt acatcagtgt ctggatgaat gtaattatct aacttactta
     9121 gtttagtttc gattttattt tttattgcat cagttaattc gatttgtttt ccagatataa
     9181 ttatgttcat aaatacatct cccttcaatt atgtcagtat aagaaaattt atactttaat
     9241 aatatattct acacgaactc tcataatcct ctttttatta tttactttta tgattttttc
     9301 tattcaattg atagaattat ttatcatttt tagttacatt gatacttatg tggattaata
     9361 ttattacaaa cacctgtgga taatgtggat aaaactgtta ataactcttg tttttgttta
     9421 tttaaagcta tttttactcc tatttctatc cacaaaaatc tccattaatt gtttattaat
     9481 atgtgaattc atgtcataag atggtaattt taggaaatta tgtgtattat atgccaatat
     9541 acctttataa ttttttaata actataaacc ctctaaaacc tccataatct atcttttctt
     9601 tcattattta tatttaaaac tacttaaaag ttataaagca tactcaacaa taatttcata
     9661 tttaatacct attaacattc atatacccaa attataatta acttactctc tttatttaaa
     9721 ctaagtattt tatattcata tactatcttg aaattatatt tacgagttgt ttaagctttt
     9781 gtctatctat atcttcaaaa ggactgttat ttattctaat cgctgtacca aaatgaaaat
     9841 tacttgcttt agttaattcc tttatctttt ctatattatt aaaattaaga cctcctccta
     9901 aaagaatttt tatgtgatta gaactaagca tcatattctt tatcatttgt atgttatcaa
     9961 ctatacttcc tttccctcct gatgttaata tattcgtaat tttatcataa tccttcaaaa
    10021 ttttaacact atctattgta tttgattcat ctatggctct atgaaatgta acatcaagat
    10081 tatcacaaca tttcaataaa acatttagat ttttctcatc tatattattg tttttatcca
    10141 atacaccaaa tacaactcca ttagcaccaa tctctttaac cacacctata tctttttgca
    10201 ttatacttat atcttcttta ctgtatacaa aacttttcgc atgatgtctt atcattacgt
    10261 tcacaggtat ctttacactg ttaacaacac tttctataag accaaaactt ggtgtcaatc
    10321 caccttcagt taaagcacta actaactcta ttctatctgc accacaatct tctataattt
    10381 tagcgtcttc tactgacatt cctattattt ctaacataac tactcctttg ctaattcata
    10441 tattgcattt gcaaatatct ttgcatttaa tattatatct tctatatcca tgtattcatc
    10501 aggattatga gctattcctt tttgtcctgg aaatgaaggt ccaaacggaa ctatatttgg
    10561 cattactttt gcatacgtac ctccattagt tgtcacggga gttccatcta atgatgtaac
    10621 cctttcataa gctagctgaa gctttttaac aagattactc tctttatcaa aatatacagg
    10681 attaaaatta gaaattacct ctatatcgat actatttgga aaactagctt ttatttcatc
    10741 taatacatct ttcaatttat aactaaaatc gtattttacc tttatatcta atatagtatt
    10801 actattttct atattcagct ctttatatgt aacaagcaca ttttcagaca tattttctat
    10861 aaaacctagg tatacatctt cttcataatt gattttactt tttattctaa tctttgccat
    10921 gcctttttca ccatatacaa caggatattt acaatctggt gtaaatccca taataggtgt
    10981 tttttctttt tctaaataat atggtatatc ttcaaatcca ctttcttcat ttgtaccaaa
    11041 tattattcta actctcttat ctaattttaa gtttaattct ttaatagcat ataatccata
    11101 taaggcagac attataggtc ctttattgtc tagtactcct cgtccataaa ttcttccatt
    11161 agtttcttct cccttataag gctgatgctt ccaaccatct ccttcatgta caacatctac
    11221 atgtccaatt acacaaacat aatcatcact atctccatgc tctgcataac caatataatt
    11281 atccaagttt ctaaccttaa atcccaattt ttttgatatt tctaatgctt tattaagtgc
    11341 ctcccctact ttttcgccaa aagggaagcc attttctgac tcccctttga cactaggtat
    11401 ttttacaata tctataatat cacttaataa atcttctttt aactcatcta ttttattact
    11461 tatgatattc ataaaaatct ccttattata aaactctatt ttaattatgt aacactactt
    11521 tactcactta cttctatcgg tgcttttatt ctagcctcat atgcttccat ccactcattt
    11581 gatataacct tatgtatagt ctttccatct actctatttg aaatatatac agcaggagat
    11641 tcttctttag ttactactga aaaagtgaaa tcaactatat ttgtagctac tccccattca
    11701 cctttattat tcatggcaac taatgataaa tcaccagctt ttcctcttct tttgataagc
    11761 tgtttatcaa gttcatttac tgcaatatca catgcttctt gtggatgctt tccttctttc
    11821 ataagcctaa ctatctcata agatatacat cccttcatta aatcttctcc caaaccagta
    11881 gctgtagctc ctcctatact actatcaaca tagaatcctg aaccagatag tgcagaatct
    11941 cctattctac ctcttttctt cataaacaat ccacttgttg atgtagctgc acacattttt
    12001 ccagttttgt ctaaagatat cataccaaca gtatcatgac cagcatatgg gcttaatcct
    12061 ttgtctaaag tctctttttt tctcttttta taatgtaact tcgctctatc agtcaacata
    12121 ttttttcttt caaatccatt tttatgagca taatcttcag cacctattcc aactaaaaaa
    12181 ttatttacct tctcataact taacttccta gctatgctga caggattaga ataatctctt
    12241 ataccagcaa ctgcgccaat atctaatgtg ttaccatcca taaatgcagc gtcaagttct
    12301 acctcacaat tttcatttgg aagtccacca tatccaaccg atttatagaa tggatagtct
    12361 tcaactcttc ttacagcaag ctctacagca tcacctgctt ccatgccttt ttctaaatgt
    12421 tctgatgcct cagttattcc ttctagggcc attctccatg tcgctatcat tccccacatg
    12481 ttaaaattcc cctccttaaa ttatatagat tttactaatg tatgcttatt ataaatagta
    12541 taatatttaa acttgctaat aattaatact cttattaata acaaaattct tctttcatca
    12601 aagttatatc ttctttattc gctattgctt ttgccaattt acatatgtta tttaatgaat
    12661 cattaagacc aattccacct tttttgggag tcttaactat acatatgtca tttttatatt
    12721 cgtttatcac atctatattt tcttctgaca tagctgcaaa agctggtata tctgttttat
    12781 catttatatt tttagacagt attgctgaca tcttagaaaa gttttcgtaa ttaaagcttg
    12841 gtccacagat tactacatca gggtttaatt tttttaccat agctatcatc ttactcgtta
    12901 cttctttttc attatttaca aaaaattcat ccccacaaaa taaggtagct attaccttag
    12961 attcatttaa aaattgctcc atcattacac ctggtcctaa tgggcttgat tttccagcag
    13021 gaggtatatt acttttctct tttcctccag ctccagcttg tatttgatcc aatatcataa
    13081 ctattttctt cataatcttc ctcctataat actaaatcat caaaagatat atcatcatct
    13141 tcctcttcaa cagtttcatt ttcttctctc aagtattgct tatctagcat ttttataaat
    13201 ggtatataca taaatattcc tagtataaga agcactaatt gtaagactgc accttgccat
    13261 cctgttgtta aaaaaccaga gaatataatt ggagttgtcc acggtaactg aactccattt
    13321 gtaagaggca ctaatcctgc tgacatacag aagtaagcta ttattatatt tattgttgga
    13381 actattgcaa atggtatcaa tagtattggg ttaagcatta ttggtagacc aaatatcaat
    13441 ggctcattta taccaaatat tcctggtaaa attgatagtt ttcccattga ctttatacgt
    13501 ttacttttac atattaataa catagctatg actaatgata gtgtactacc tgctccacca
    13561 aatgtcgcaa acatatcttg gaattgacct gttattatgt ttggtatttt tgaaccagcc
    13621 tgaaaagcag ctaagttttc agctgataga atttttaata ttggattata cacagcccca
    13681 acaacactac ttccatttat tccaaagaac cagaataaat gtaagaatat ataagcgatt
    13741 gccatagcac ctaaactatt tcctaatttt aataatggca tttgtaaaaa cttaaatata
    13801 aattcatgta tacttccata tggtgtagct tcaaaagcta tcttaactaa aaagaaaact
    13861 accataacta ttgcacttgg tattaatgct gcaaatgatt ttgatacggt tggaggaact
    13921 ccatctggca ttttcaatgt ccatcctttt tttacaactg cagcaaataa ttttacagag
    13981 gttattgctg ttatcatacc tacaaacatt cccttagagc ccatccaacc tagtggtata
    14041 cctgttacca tgtagactga ttctgttcct tctggtgtat atggtattac aaacggtgtt
    14101 actataaaaa aggcaactat tgcaactgca gctgatgcta ttggatctac atctatttgc
    14161 tttgcataag aatatccaat tcctaaaact gcaagtaaag tcattacttc aaatgttgct
    14221 acagttggtt gttgcaattt tacagcccaa tctggaccaa aaatttgacc tacaaattca
    14281 ttccatcctt ttataggaaa atttgctact aataaaaata atgaacctat tattaataat
    14341 ggtgatgata ttaaaaaacc atctctaatt gcaattaata atttattttt acctattttt
    14401 tcagccaatg gcataagtat tctttccaat ctattcatct atccaccctc atttcctatt
    14461 attttttact ataattctac gcttgtttag attttattag ctttatagca agttttaata
    14521 ctttttcccc attcatagtt ccataatcaa cttcattgat tatatctata agttttcctt
    14581 gagcttcaaa ctttggtttt aactccttta gcatgtattt aacttgtggt cctaataata
    14641 tacaatctgg atttttttct tcaactattt tgtcaatttc tgctatcgga aatgcctcta
    14701 cttctattgg taaattgtgt tcatctgcta ccttctgcat tttatctgca agtaaacttg
    14761 ttgacatacc aaagctacaa aataaatata cttttctttt cattctttta aatccccctt
    14821 aattttttta ttttaatttt tgaatttctt ttcttaacat aatcatttct tttataaggt
    14881 ttctctcact catacaagtc attaaatggt cttgagagtg aatcaataaa atagatatat
    14941 ctgatttatc tcccccagct tccttttgta taagttcagt ttgtacttta tgtgctttta
    15001 aaatttcttc atttgcattt tcaattaatt catctgcttt tttaaaatcg ttttcctttg
    15061 catagtcaag tgcttcataa agcatagatt ttgcatttcc agcatgaatt ataatcttta
    15121 aaactatttc ttccattttt tcctcctaaa ttgcttctct acttttagat tatttatacc
    15181 ttacaaacat ataatatctt ataaaaacaa aaaaacatgc acaacttttt ccatcacatg
    15241 gaaaattttg tgcttatcca tagttcatta tgaattttat ataagaatat cttttacctt
    15301 ttcaatgtca catttactta gaatatctag catattttca tcctctgtta aattagatat
    15361 ttctttaaat aattctctaa atacttttct atcatcatct tttttatatg ctaataaaag
    15421 tataatcttt atatcctttc catttctaat ttttattggt gtttttaacc ttgcaaaaca
    15481 taaaacagat tgttttatat atttcatgtc tccatgaggg aataatattc cttttccaac
    15541 attggttgga tatgactttt ctcttgttat aatagattgt aaatactctt tttctgcata
    15601 atgtttttca attaataaat tagttaaata tgttatagct tcataaatat catcatgttc
    15661 taactctctt attaaataat cagacatcat taatctgttc ataagtttat atgaggtgtc
    15721 tttttctttt attttgtttg taaactcttt aagtttattc tcatcctcat cagtaaataa
    15781 tggtgttgtt ataaaaattg gtttttggat attttcatac tcttgaaatg caattataaa
    15841 atctatagtc ttattaaagt tatctctttt taaatcttcc tctcttaatg tttcaataat
    15901 ttttatgtta tcaaatctct tttctatctt tacttttaat agcatactta ctccaaaact
    15961 aaatggacat acaatacttg cctttttatt tgattccctt ttgctgtatt ttctttctag
    16021 agaggtttga aagtgaagtg taagataacc aatttcatct tccgttattt gttttccttt
    16081 taattcaatt tctattgaac ttgatattac tgtaaataaa aaacaaaatt tagtttttat
    16141 gttatctaaa agtggatttt ctaagtaaac attgcttttc atttgatgca ttgtaacatt
    16201 caaatgacat attaattgtt cataaagtaa tatatcatta ttaaaattta ttcctgattc
    16261 ttctgatact aaatttataa ttttttttgt ataacttgct aattcttcat ttagatataa
    16321 agatttctta atattattac tatttatttg tttgttcata cccaatatta aagattctat
    16381 aaacaatttt tcttcataat ttaatactat ggataaatta gatttcaatt catcttctat
    16441 atccaatata agcattttta gcttaagctc tttcttagta tttgatactt ttaaagaatt
    16501 tcccagcctt attcgtatta tatttataag caaaaatagc atcaattgct tgaatgatat
    16561 atctgtaagt cttatatcca tatcctcctc tatagtaagc attatgtttt ttacaattat
    16621 tatatctact atatggaagt actctatttc atctatgttt aatctgtatt tatctatgta
    16681 tttaaataag aatgatacta acattattct tatgtctttc tcatcccctt caatatatat
    16741 accactgcct tttttagtcg agacatttaa attataatta tttaatgcta ctgataattt
    16801 acttatttct tctctgacaa tatttttatt tatgtacaac ttattagata aatcatttaa
    16861 agtaactttt ttattattac aaagtagtaa atttaatatc tcaacttgct tatgaagctc
    16921 actatttact cttttaactt cacctcgcaa cttctgattt accagtattt tgtctgttcc
    16981 atctccacta aatttaattc caatattagg ttttcttttg atataagcat tacaaaaagt
    17041 agataaccat tcatcaatat atttgcaatc attccttatt gtcctttctg aacactcaaa
    17101 atcattggca atcttattaa catttataaa ctcttctttt aatagcaatg ttgttaataa
    17161 attttcttgt cttttattca aacatatcac ctctttatga ctcaacctaa atttattata
    17221 tataaaaaag agctatacat aaaggtatgc tagctctctt ttaatatgat atttagctga
    17281 cctggttttt gaccccacac tcgcctactg gagggttcgc ttgggttcgc cgcactacta
    17341 ctctctctcg attttattat cggtaggtct tacttctaag ccgcaccatg atcattaacc
    17401 caatttttag tgttaactta cgtggatccc tttgcctgcg actggcttgg actttcaacc
    17461 acagacctaa atatcactta ttcattttaa tattttaaac taaaattgtc aatatataag
    17521 attattccat aatataatta tcatttgctt ttgtaagtag agtcaataca aaaaccttat
    17581 tagcacctga taactttaaa agtttagaga tttcgtttgt tgttagacca gtagtaaata
    17641 tgtcatctat taaaagtata tttttattat ttattaattt aacattttct ttaacaacaa
    17701 atacattttt taattcttct tttcgttctt tcttattcaa tttatatagc attcttgtat
    17761 atttttttct acttatgcaa tctaataatg gaatatttac tattttactt aaattaaatg
    17821 ctattttttg tgcctgatta aaacctcttt tatttagcct ttttttatga agtggaacaa
    17881 ataatatata atcaaatttt atgttttcta aatataattt ttctttcatt atttgagcta
    17941 tatacttagc cataaaggtt ttttggttat acttcagccc taacaccatc ttttttgtca
    18001 catcattata ttcaatacaa gatattgatt tatcaaaata aaagtcttta ttaaaacaat
    18061 aactacactc ttcaataaat tctttttcaa ttgaatgacg tattattggt tttccacact
    18121 tcatgcaacc atcttgtata aaattcattt ctttaaaaca acttttacat attgaataag
    18181 tatttgtttt ctttatggat ttatcacaaa ttatgcaact tatattctca ggatatataa
    18241 aatccaaaca tttattaatc atatttttaa aattcttaat aaaattatca ttgcatattt
    18301 tcatatttat tctatcaaca acccttcttg tttaaattta tttagcttat atcctaaatt
    18361 tgaatacctt tgattaactc tattattttt tatcatatat tcaagatatt tcacatctcc
    18421 aactaataca actaattttt ttgctcttgt tactgctgta tataataaat tacggctaag
    18481 taacatagga ggagcccacg ctattggcaa tactacaact ggaaattcac ttccctggct
    18541 tttatgtatt gttgtacaaa aactatggtc aatttcatct aattcatcat ataggtatga
    18601 tacaatttta gtctggtcaa ataatatata aatagttttc ttatctttat ctatgtgata
    18661 aacatatcct atatctccat tatagatacc ttctccactt tctttttggt cttctgtttc
    18721 ccattttttt gtataattat tttttacttg cattacctta tcgccaactc taaataatct
    18781 tttagaaaaa ctttcttcaa ctttaaattt ttcttttttg ttaagatact tctgtaactc
    18841 tatattgaga ttagtaactc ccaattcacc ttttctcatt gaagataaaa cttgtatatc
    18901 ttttaattta tcaactttat aaaatttagg taatctttca ttaaccaatc ctattatttc
    18961 atttaaaatt tcttcattag ttgattttct tataaaaaag aaatctttac cttttgtatt
    19021 tagataaagt ggttctccat tgtttatctt atgggcattt acaattatca tactttcttg
    19081 cgcttgtcta aatatttcat ttaatttaac tacatttata atattagaat taatcatatc
    19141 cttaagtaca tttccagcac ctacagaagg taattggtca ctatctccaa ctaaaattac
    19201 tcttgttcct aactttatag ctctaagtaa attgtacatt aagataatgt ctaccattga
    19261 tacctcatca acaattatta catctgaatt tattgggtct tcttcatcct taaaaaaagt
    19321 taaatcatca tctgttgaaa atcccatttc tagcaaccta tgtatagttt ttgcttcttt
    19381 attagaagtt tcactcattc tctttgctgc tcttcccgtt ggagctgcca aagtcacact
    19441 tttaccatta ttttcaaata tatctatgat agcattaatg gtagttgttt taccagttcc
    19501 tggtccacca gttataatta atactccact attaacagat tctttgactg ctaaaatttg
    19561 tttttctgct aacttaattt tcttatcttc ttctaaaaca ttaatctcgt tatctatatc
    19621 tatttttaag tcttcaaatt catactgtga taattttact atttgactac atactccatt
    19681 ttcagctaaa tagtatggca taaggtatat aagattttca ttgtttactt tctctatatg
    19741 tattttttga ttataaacta gcatcattac acactctttt attatttctc catttagctc
    19801 caataatttt tccgaatctt gtattagaac atgctctggt aaatatgtat gcccattact
    19861 taaagattta tttaaagtgt ataagattcc ctgacaaatt ctatctttag agtttttatc
    19921 tataccaatt ttacttgcta tactatcagc tactttaaat ccaattcctc ttatatcttc
    19981 agcaagttgg tatgggtttt tatttataac ttctattgca ctgcttttat attttttata
    20041 tatttttagg cagtaatttg gtgttattcc aaaaggagat agctgtatta ttatatttct
    20101 caattctctg tcttcttcat aactttttac tatttgtttt acttttttac tccctatacc
    20161 ttctacttct tgtaattttt ctggtgaatt ttgtatcacc tctaacgtat ctacaccaaa
    20221 cttatcaatt attctttttg ccattttttc accaatacca tgtatcattc cagaagataa
    20281 gtaaacataa attccttcta aggatgatgg cgttactggc ataaaactat tcacttcaaa
    20341 ttgagtccca taaatcttat gatttaccca ctttccttca acttctatgc tttctccaat
    20401 tgccaaagta ggcatacatc ctacaaccac aatttcatca ttttcattta ccaggtgagc
    20461 tattgtataa ccattatctt cgtttttaaa gactatttca cttatcattc cttctaattt
    20521 ttccattttt atctcctaaa aatactaata atcgctccat ttaaatgtta gtataatttt
    20581 atatattaaa actatgataa tcgtagaaaa gttttatatt tttatactta acgtaatttc
    20641 caactattat acattttatc agaaacaaaa aaaatctgca tccataaaat atggtaccat
    20701 gaaatacagg taccatattt aaatagctat tttattttaa ttactctata tttagagtat
    20761 aaattaaatt ccataagtat attacagtac taacttattt cagatttatt ctccttgtac
    20821 ccctcagttt tagtaattct tctataaagc atacatagta aaattaaaat tcccatgtaa
    20881 cctgtatatg gatatagtgt acctactaac ttatcaaatg gcagtaaacc tcctatacaa
    20941 gctaatatac ttaccactat tgttataatt ttaaatttag ggtggtcatc ttctacaaat
    21001 cggttagtta ctgaccataa caatggtaca gctgtagtgt atattcctag taaaagtact
    21061 actgagaata aaatacctat tattggagat atcttatcag ctaagtaaag tgctggtatc
    21121 tcttttgtgt atatatttcc tatgtttgaa agtaatgcca aattcatcat tatagctgct
    21181 gccataagtg ctactccacc tactatacca ccccatacag catctttctt atttactgca
    21241 gaagctccca ttcctgtcaa gaatgttatt actattatta cattgtaaca agtataaagt
    21301 actcccgaga aatatccatt tgatgtagca gttttaatat ttaaactatc aaccatattt
    21361 ccagcatttg ataaagcatc aatattgcta aataaactaa ttgctcctac taaaagtgta
    21421 aatattatta ttataggtcc tatattacct agtattttag aaagccttgt caaacctaat
    21481 gaaacagtta ttagggaaac gattgccata ccaatcctac ctacatatgg atttaatcca
    21541 tagtactctg atagtgtagc tcctgctcct gcaatcatta ttactaatgt tccaaataaa
    21601 aacaatggac caaaccattc aaaaaatgtt ccaagatatt ttccacaata tacttgataa
    21661 atttttattg gctccttaag tttcaattct cttcccttat ctataacttc tgctccaaac
    21721 catgaaaata gtaccataga gattattcca cctataatac ccgcatatcc ataaaacgta
    21781 aaaaattgca gtatttcttg accagttgca aatccagaac ctattacagt agcaacataa
    21841 gctccagcaa aacttataac cgttttaata tttactttgc ctgacataca ctactccccc
    21901 ccaattaaaa ttatttttaa agtgtaagtt tattaagatt atattagact cattattgtg
    21961 atatttcaat atatttttaa ttatttaaca ttagttaaca cattcttaac catcctttaa
    22021 catatatccc ataatatgta taaatttagt agagtattat ataaaatgta ttgaccagat
    22081 taaatactgt ttattcatta tatgatgata aattaacatt tattaaaaat atttaactca
    22141 taataaaaaa actgttagaa agtaatcgta aaattacatt ctaacagctt tttagttaaa
    22201 aattattcac ctaaagcatc ttttcttaac tgctcaacct tgtcagttct ttcccaaggt
    22261 aaatctatat cagttctacc aaagtgacca taagcagcaa cctttttata aagaggcttt
    22321 cttaaatcta agtctcttat tatcgctccc ggtcttaaat caaagtgttt atttactaat
    22381 tcaacaagtt tttcttctga aaccttgcct gtcccaaaag tatctataaa tatagataat
    22441 ggtctagcta tacctatagc atatgcaagt tctatctcac atttgtctgc aagaccagcc
    22501 gcaacaatat tttttgcaac atatctagct gcataagcag cagatctatc aacttttgta
    22561 gggtcttttc ctgagaaagc tcctccacca tgtctagaat atccaccata agtatctatt
    22621 attatttttc ttcctgtaag acctgtatct ccttgtggtc ctcctataac gaatcttcct
    22681 gttggattta tataaatttt agtctcttca tcaagtaatt ctgctggtat aacttcctta
    22741 ataacatgat taattaaatc ttctcttatt ttatcatttg atactgtttc acaatgttga
    22801 gctgatataa gaactgtatg tactcttaca gctttacttc cttcatattc aacagtaact
    22861 tgagtttttc catctggtct taaataatca actaaacctg tttttctaac ttctgtaagc
    22921 cttcttgata atttatgtgc caaagatatc ggaagtggca ttaattctgg agtctcatta
    22981 catgcaaatc caaacattat accttggtct ccagctccaa ctttttctat ttcttcttct
    23041 atctcttctc cagtcttact ttctagacct tcatcaactc ccatagctat atctccagat
    23101 tgttcatcaa tagaagttat aactgcgcaa gtatcacaat caaatccata ttttgctctt
    23161 gtatatccaa tttctcttac tgtttctctt actaatttag gaatgtctac ataagcagat
    23221 gtacttattt ctcctgctac taaaactaaa ccagtagtaa ctgtagtttc acaagctact
    23281 ctagattgtg ggtctttttc taataatgca tctaatattg aatctgatat ttggtcacat
    23341 atcttatcag gatgtccttc tgtaactgat tctgacgtaa ataaatgtct tgccattttt
    23401 attccccctt atttattctt aatttttatt ataggtttta ccttattttt aattttagaa
    23461 atttactcaa aataaaaaaa cctctccttt ataagaagag gttaataatc gtaattacat
    23521 actaattaac tgacctcatc tttcaagact tttgtcttgt aggaattagc accttttccc
    23581 atttgggtgg ttgctgggct tcacagggcc tatccctcag cctctcttga taaggtaatc
    23641 aagatttaag ttttgtttta ctttactagc ttactatttt tttaaaattt tgtcaatatt
    23701 tttagtcata ttttttctat tccttttttt gtaagacaaa taaaattttc tatctatctg
    23761 aaatttaaag ttaggtcttt ttaatgcttt ttattcatat atttttttga agggggtgtt
    23821 cctattgtat ttagctaaag taaactacag cgatgatgat gttatacaga tgctaagttc
    23881 agttctaaaa actataatta atgaaaatac gattgttgtt tgcataggaa cagacagggc
    23941 tataggtgat actttaggcc ctttagttgg aacaatactt aagaacagta actttaaata
    24001 ccccgtttac ggtacactag ataaccctat tcacgcctta aatatatatg agtctttaga
    24061 tactataaaa aatacacata tacaaggtaa cttcttggct atagatgctt gcttggggtc
    24121 gcagagtaac ataggtaata tacagattag agaaggacct atcctgcccg gaaaaggtgt
    24181 tggtaaaaaa ctacctcaaa taggaaatta ttctatagta ggaattgtag ataaaattga
    24241 tgaaaataat aaactttcat ttaataatat acgccttagc tttatattag atttagctga
    24301 aacaatagcc ttggctttat tagtatccac ctaatctaaa tataaactaa atgataaaat
    24361 tctttatata aaaaggtaga tattttagag tatctgatta aatactccaa attatctacc
    24421 tttttacttt tatatttcaa gtttttattt ttctactaaa gcaaacatta attcaccttt
    24481 acagacaagt tcttcaccta catatgcctt agcatctgcc ttagctatat tttttctaag
    24541 agttgtcatt tcaatttcca tagttaatgt atctcctggt cttacttctc tcttgattct
    24601 aactttatct atacctgcaa atacacctag ttttcctttg ttttcctcta cagacatcat
    24661 tgccactcca catacttgtg ctagagcttc tactattagt actcctggca tcaaaggata
    24721 ttctggaaag tgaccttgga aaaatggttc attaactgtt acattcttta taccaacagc
    24781 tcttttccca acttctagct ctgtaatctt gtctactaat aaaaatggat atctatgtgg
    24841 tattaatttt tttatttcat ctatatttaa cattctaaac tctcctaatt attttctttt
    24901 gaaagaccca ccagtttcaa gtaagtctat tgaacctaaa gctattcctg taccttttgc
    24961 aacacaagat aatggttcat ctgctattgt aacagttatt cctgtcctct gttctattat
    25021 tttatctaat ccatataata atgaacctcc tccagtcatt attataccga catctcctat
    25081 atctgctgct aactcaggag gcgttttttc caaaacagca tgagtagcaa ctactatttg
    25141 ctcaacacat tctctaagtg cttccaacat ttctgttgaa tttatagtta tagaactagg
    25201 aagtcctgta attaaatttc ttcctgttat cttcatatat ttttcttctt ctctcttaaa
    25261 tgcagaacct atttcaaact tgattttctc tgctgttctt tctcctataa gaagattgtg
    25321 ttgttttttc atatatttaa ctatagcatc atcaaatcta tctccaccaa cctttataga
    25381 ttcacttaca acagctcctc ctagagatat tacagctata tctacagtac ctccaccaat
    25441 atctataatc atattaccat taggctgtga tatgtctaca ccagctccta tggcagctgc
    25501 tattggttct tctatgatat atacttctct agctccagct tgtctcgtag catcttctac
    25561 tgctcttttt tccacttctg tgacacctgt tggtacacat accataattc ttggcataaa
    25621 aaatcttcca aatccctttt tatcaactat cttatctata aaagatttta acatcttctc
    25681 agttacatca taatctgaaa ttacaccatc tcttaaaggt cttatagcga ctatattacc
    25741 tggtgttctc cctatcatct ttttagcttc ttctcccaca gctaaaacag aatctgttct
    25801 tttgtctatc gccacaacag atggttcttc taaaacgatt cccttaccac taacataaac
    25861 aagcacattt gcagtaccta aatctatgcc tatatcagct ccagccattt tttcaactcc
    25921 tttatttata atattagatt atattataag cctattttaa tactcctata ataaagcata
    25981 tacaaaaatc tcgctttaat caagagaaaa taaatctttt gagagtatta ttattttgct
    26041 atttataatt acttaatata tttcttttta taatttaatt caataaaaaa agtaggtttt
    26101 taacctacat ttttttatgt tccttctcat attttaattt cgttgctaat ccccctctaa
    26161 tatgtctctc tgatttattt ttgtccagca catttttaac ttctttggca agggatggat
    26221 ttatttcctt caatctttct gtaacatcct tatgaattgt acttttactt actccaaatg
    26281 tcttggcggt ttgtcttact gtagtgtttt tttcaagtat atattttgct accactatgg
    26341 ccctttcctc tatatgagat ctcaaagatt acccccctca cataatagct ttaatgatat
    26401 atatgtaaaa acaccaaaaa atataacaag cttaaccaaa tatgattaaa cttgttatac
    26461 ttttacttaa ttagactcat tgggtctata gatttttctc cactatacgc ctctaaatga
    26521 acatgtatac cttcttcact ttctatttga gaagtactac ctacagtacc tagagattgt
    26581 ccttctgtta ccttctgttc tttcttaaca ctaacatttt tatctagatt tgaatatact
    26641 actacaatgt tattgtcact ttttattttg actgattgcc catattcttc atcattaaat
    26701 acatctacta ctattccgtt taatagagat tttacttctt tgcctttagt acaactaata
    26761 tctacacctt tatgagtttc ccaaacatca agagttttag aataactagg ttctttttct
    26821 gaatatcctc ttattacttt atctccaatg taatttaatt ttgttgtact cgatttattt
    26881 tcctcttttg acttagcttt ttctaagttt tgttttgaat ccgttgcagt agggatagca
    26941 tctttcttgt ctttttcaat taaatgtatt tcctcttcac tatctttatt agcattttcc
    27001 ataattcctt tattagaagc caatttatct acattattat ttgtaaacca aactccacct
    27061 actgctaata aacaaacaca tacaaacaaa gataaataaa aaccatcttt ttctaacagc
    27121 tttttcttca ttttgcacct ccaaatatat ttctacattt ggattattga cgtgtttttg
    27181 gcattttata caattattag tatatatctt ttatttttgt atctgtgtag taatgtgata
    27241 atatatcata atatttatag ccctcttctg ccattccttc tgctccccac tggctcatac
    27301 caacaccatg accatatcct tttaccacga aatctatgta tccttctcca aattttatgt
    27361 caaagtttgc agagttcaat ttaaaaacag ttctaatgtc ttttccagtc aattctttat
    27421 ttcctaattt tattttttct acagttccgg catcacttct tttagttatt gaaacttgtt
    27481 tacttaaatt attcacatct atcacaatag tgctatatgc ccttctaaga cttttaacaa
    27541 aatctgtgtt acttatttta agtgttgacg caaatttagg ggaatactta tcataaggac
    27601 tttccacaga ctttaaataa ggatattttg cagaaaatac ttcttcacta ttttctgttt
    27661 ttcctgaaga tgtagaaaaa taaagaggaa gtatcgcctt atcattataa gttattattt
    27721 gacctttagt tcctctaact gcttcttgga ttttggaata tttattctta atccattctt
    27781 caccatttaa ttttttaaga gtatcataac tcttatattc ttgacaatgt ttataatcag
    27841 tacatactac tgcattttta tgtttactag atttaccatg ttcctgttta tatactacat
    27901 atgttctagc tgctacagat tgagctttta aagcttctat atcaaactct gaagacatct
    27961 caccagctag tactccacat aaatagttct ccatgtccat cttttcggtt ttccctgtta
    28021 tatgattata tacattgata attggtgctt ttttatttac agtttcatag ctcactgtac
    28081 ttttttcttc tttattacct ttgtcttcaa tatcactttt tttaatagtt ttgtttatag
    28141 atactgggct ctctggtttt tcagttaatt ctacattttt ataagaaact aaagttataa
    28201 gtgatggaac taacacagag caagtcacaa atcctaacaa aacaaccaat gggttcttca
    28261 tatactctcc tccttagctt ttaaaacctt gtatttaagt atatgaacaa ccctattaat
    28321 ttataacttt cttctataaa ccaattatta atcttctact atttcgattt gaccacctag
    28381 tgctgttatc ttcttatcta tatcaacata acctctttgg atatggtata tttctcctat
    28441 ttgagtttct ccttctgcta taagaccgca taaaataagg gctgcccccg ctcttaaatc
    28501 agtagcgttt actgctgcac cgtgtagctc atctacacca ttaacaaccg cacttcttcc
    28561 atctattttt atattagcac ccattctatt aaattcagca acatgcataa atctattttc
    28621 aaaaacagtt tctataacta caccagttcc attggctaca gtaagcatag ccataaattg
    28681 tgcttgaaca tctgtaggaa atcctggatg tggtaaagtt tttatatcta ttggttttaa
    28741 tacttttgga cctacaactc ttacagaatt gtccatttct gttatttcgc aaccagcttc
    28801 tcttaattta gctacaactg gttttaaatg ctccattaaa acattttcaa cagttatatc
    28861 accttttgtc atagctgctg caaccatata agttgctgct tctattctgt ctggaattac
    28921 attatgttct gctcctttta actctttaac accttttatt tttatagtat tagtacctgc
    28981 acctttaaca tctgctccca tttcgttcaa gaagtttgct aaatctacta tttctggctc
    29041 ttctgctgca ttctctatta ttgtagtccc ttctgctaaa gaagctgcca tcattatgtt
    29101 ttcagttgct ccaacagatg ggaaatctaa atataattta tttcctacta acttttctgt
    29161 tgctgcttca acaaaaccat ggtccatttc tatctttgca cctaaggctt taaatccttt
    29221 taaatgtaaa tctataggtc ttgttcctat agcacatcct cctggcatag aaattttcgt
    29281 actattaaat ctagcaagca atggtcccat aaccaaaaaa gaagctctca tctttcttac
    29341 aagctcatat ggagcttcac aagtttttat attgcttgca tcaacagtaa gtgtattttc
    29401 tttatattca acttctgctc ctacatgtct taataaatct gatataacat gaacatctct
    29461 taaatttggt actccattta atgttgattt tccgtttgct aataaagtcg ctgctattat
    29521 tggtaaaact gcattttttg caccatctat tttaacacta cctttaagtg gattactctt
    29581 ttttactatt atcttagcca tattttttga tctcctcata attaataatc taattttata
    29641 attggtgtgg caatatatat gagtgttttt ccgtcatcct cattatattt aattcctata
    29701 tttaaattaa ttttttcccc taaatattct aattcataat catttaatac tttactatat
    29761 gctgttatag ataaaaagtt tacgtcttta acttcatcta tttgcttagc actcatatta
    29821 tataatattt tttgtaaaat atcatcatat ttgttgtttt gtaacctttt tgtgtattct
    29881 ccaacaatac aagcagacac atctacttga gatgaatatt tatttaacac attttctacg
    29941 ttactataaa taacctcttt atgtttatat actttgttat ccaatatgtc aactactatg
    30001 taactttctt tgttattttt atttattcct tttatagaaa tttctttatc gctacctttt
    30061 atagtagcac ataattgtat tttatcgcca gacccttttt gattccaatt tattcctttt
    30121 ttatccaact ttagattgtt aattatttct ataaaaatat tatgaaattc tttatttgat
    30181 aaattatgat ttataacaga attaatttta acattat
//
LOCUS       DACTGW010000023        30005 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.023, whole genome shotgun sequence.
ACCESSION   DACTGW010000023 DACTGW010000000
VERSION     DACTGW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30005
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.023"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(531..851)
                     /locus_tag="I9573_000817"
     CDS             complement(531..851)
                     /locus_tag="I9573_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAT4795451.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(971..1708)
                     /locus_tag="I9573_000818"
     CDS             complement(971..1708)
                     /locus_tag="I9573_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAT4795452.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1923..3746
                     /gene="acd"
                     /locus_tag="I9573_000819"
     CDS             1923..3746
                     /gene="acd"
                     /locus_tag="I9573_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HAT4795453.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYIS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3961..8259
                     /locus_tag="I9573_000820"
     CDS             3961..8259
                     /locus_tag="I9573_000820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAT4795454.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEILPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8463..8924
                     /locus_tag="I9573_000821"
     CDS             8463..8924
                     /locus_tag="I9573_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAT4795455.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8954..10108
                     /gene="nusA"
                     /locus_tag="I9573_000822"
     CDS             8954..10108
                     /gene="nusA"
                     /locus_tag="I9573_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAT4795456.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVKSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELGTSEDNFSEEE"
     gene            10115..10396
                     /locus_tag="I9573_000823"
     CDS             10115..10396
                     /locus_tag="I9573_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAT4795457.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10386..10697
                     /locus_tag="I9573_000824"
     CDS             10386..10697
                     /locus_tag="I9573_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7ae"
                     /protein_id="HAT4795458.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10719..12659
                     /gene="infB"
                     /locus_tag="I9573_000825"
     CDS             10719..12659
                     /gene="infB"
                     /locus_tag="I9573_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAT4795459.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12702..13082
                     /gene="rbfA"
                     /locus_tag="I9573_000826"
     CDS             12702..13082
                     /gene="rbfA"
                     /locus_tag="I9573_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAT4795460.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDNLIKRVNEKNAQQNEDENYDE"
     gene            13063..14046
                     /locus_tag="I9573_000827"
     CDS             13063..14046
                     /locus_tag="I9573_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAT4795461.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNVGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEGVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQTKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSDYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            14059..14964
                     /gene="truB"
                     /locus_tag="I9573_000828"
     CDS             14059..14964
                     /gene="truB"
                     /locus_tag="I9573_000828"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAT4795462.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEVVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            15095..16024
                     /locus_tag="I9573_000829"
     CDS             15095..16024
                     /locus_tag="I9573_000829"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAT4795463.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEYAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            16128..16385
                     /gene="rpsO"
                     /locus_tag="I9573_000830"
     CDS             16128..16385
                     /gene="rpsO"
                     /locus_tag="I9573_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAT4795464.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            16625..17470
                     /locus_tag="I9573_000831"
     CDS             16625..17470
                     /locus_tag="I9573_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAT4795465.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            17595..19706
                     /gene="pnp"
                     /locus_tag="I9573_000832"
     CDS             17595..19706
                     /gene="pnp"
                     /locus_tag="I9573_000832"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAT4795466.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            19972..20715
                     /locus_tag="I9573_000833"
     CDS             19972..20715
                     /locus_tag="I9573_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAT4795467.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            20873..22120
                     /locus_tag="I9573_000834"
     CDS             20873..22120
                     /locus_tag="I9573_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAT4795468.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAICLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            22155..22400
                     /locus_tag="I9573_000835"
     CDS             22155..22400
                     /locus_tag="I9573_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HAT4795469.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            22410..23633
                     /gene="dapG"
                     /locus_tag="I9573_000836"
     CDS             22410..23633
                     /gene="dapG"
                     /locus_tag="I9573_000836"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAT4795470.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEENIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            23746..24480
                     /locus_tag="I9573_000837"
     CDS             23746..24480
                     /locus_tag="I9573_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease"
                     /protein_id="HAT4795471.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            24603..27014
                     /locus_tag="I9573_000838"
     CDS             24603..27014
                     /locus_tag="I9573_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAT4795472.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKSNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKSSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            27017..28075
                     /gene="mnmH"
                     /locus_tag="I9573_000839"
     CDS             27017..28075
                     /gene="mnmH"
                     /locus_tag="I9573_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAT4795473.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESVFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            28069..29403
                     /gene="rimO"
                     /locus_tag="I9573_000840"
     CDS             28069..29403
                     /gene="rimO"
                     /locus_tag="I9573_000840"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAT4795474.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFSELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            29390..29932
                     /gene="pgsA"
                     /locus_tag="I9573_000841"
     CDS             29390..29932
                     /gene="pgsA"
                     /locus_tag="I9573_000841"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAT4795475.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
BASE COUNT        12108 a         3187 c         5700 g         9010 t
ORIGIN      
        1 tatatctcaa tttaatatat aaaaaagata ataatagtta gactttttat atgaatttta
       61 ataggtaaaa tatcctattg ttttgtagat atatcctaat tatgtcaaaa aataaaaaaa
      121 tttttattta ttagagtttt tggaaataat aataacagat attataatta attaacagtt
      181 tttgtaagta tttgacaata gagagggtgt aacaaatggg aataatagag tcagcatcaa
      241 aattggctga gatggttcat ttattggctg ttgaaaaggg gataactgat atagaagcat
      301 gggatgaagc tgtgaaagaa tacagcaaaa tttatgaaga aagaagaaat gaatagaatg
      361 ataaaaaatg aaaacttgat gataaaaaat aactttattt aaagaaattc aatttttata
      421 gaaatattaa aaataatata aataaaaaga tggtatttca aatgtaagta aaaaatcatt
      481 ttgggatacc atccttatta tttattttaa taatatataa gtaataaatc ttacttatcc
      541 atatttttta tgtaacctct tttttctagc ttttttatat gctccttacg tatttcatca
      601 agattaatac catattgttc ttctagctta aacatcatag taacacatgt ctgagctaca
      661 tccattagtt ccttagcagt tttctttata atatcaactt catctaaatc attattttct
      721 ccacttagat ttctgaattt acctatacat tcagctaatt caccttgttc ttcacccatt
      781 ttgataaatg tagattctag tgttgggtct aaattattaa gctctggtaa agatatagtt
      841 tttagtttca taaaagctcc tttcaagtta ttcgtattta tactaaaaca tcataacaaa
      901 aataatacta aaaaaaaact ttaaattcaa cataaaaact tacatatata ataaaaatcg
      961 tgaaataaat ctatatttgt cttaatacag cctcatataa aacttctcca ataggtttat
     1021 ttattacaag agaagctgac ttatcataga ctgtatttgc tttattaata agagcaatat
     1081 aatcaccttt gtaataatct ataaaacttg ctgctgggta cacagctaat gatgtacctc
     1141 ctataatcaa taaatcagca ttacttatag cagaaattgt tttagtaatg acatcactgt
     1201 caagagcttc ctcataaagt actacatctg gttttactat agaaccacag ttgtcacaat
     1261 aaggaatatt acctcccaga tttaacatag attctaagtc aaaaaactta ccacatttag
     1321 tacaataatt tctatgtaca gaaccatgaa gttctagcac atttttactt ccagccattt
     1381 gatgaagtcc atcaatattt tgagttatga cagctttaag cttacccatt tcctcgagtt
     1441 ttgctaaagc tatatgagca ttgttgggtt ttgcattagg atatattaat ttatccttat
     1501 aaaattcaaa aaattcttct ggatatctaa caaaaaaagt atgtgaaact aattgttcag
     1561 cagtaaattg tttattgagt ttttggctaa ataatccagt agaacttcta aagtctggaa
     1621 tattagattc agttgaaaca cctgctccac caaaaaatac aatattattg tgattagcta
     1681 ttaagtcttt taagctattt gcatccatag ttacacctcc aaattattat atataaattg
     1741 tactacatat taggtctttt atgttttatt aatttaaata aatatgtagt ttaataagtt
     1801 aaataaaggg aatataaata aaatgtatac agagtataaa tagacaatat ttactaaaat
     1861 aacaagttgt cgtataatta tattaaaatt gttttagtgt ataagaaggg cgggggaagt
     1921 acgtgaagaa aaaagcagct ttagcaacat tggctatgtt acccttaggt gtggtaaatg
     1981 cacatgctga tggagatata ggtatagtga ctataaatta tttaaatgta agaaatgagc
     2041 caactgccga aagtagcata gcctttgttg ctaaaaaaga tgataaagtt ttgattaaag
     2101 actcttctaa tggatggtat aaaataaaag ctgaatctgg acaagaaggt tgggcttcat
     2161 caaaatatat agcaaaatca aatagtgact ctttaagaac gtctacaaat aaagaaaaac
     2221 aagttatttc aaatagttta aatatgagaa atggagcagg tacaagttac agagtaataa
     2281 ctgtactaaa aaaaggtcaa aaagtagaag taatatcaga gagtaatgga tggtctaaaa
     2341 tcaaatatga tggaagacta ggatatgtat ctagctctta tttaggagac gtttcaaatt
     2401 caactaataa atcaaaaact aaacaagtga atacaacttc actaaatgta aggagtggac
     2461 caaatacaag ttatggtttg ttaggcaagt tgccaaaagg aagtaaagta gaagtaatat
     2521 cagaaagtaa tggatggtcc aaaataaagt ataatggaaa agatgcctat atatctagta
     2581 tgtacttatc ggatgtaagt caaagtaatt cagacaattc tagtcaaagc aatgataaaa
     2641 agaatactga taaggttgta aatacagctt ctttaaatgt aagaagtgga ccaggttcta
     2701 catatagtaa gttgggaaaa gtttataaag gaagtaaagt aactgtacta tcagaaagta
     2761 gtggatgggc taagattaat tttaacaata aagaagcatt tgtagtaggc aattatttat
     2821 ctacttcagc agatacttca aataataact caaatagtaa ctctgataac agttctaata
     2881 gtaatggcaa taattcttca tcatcaggtc aagtaaatgg tatgtcaggt ataagtgggg
     2941 ctaaaattga ttataaatct ttgagttata ctttagaatc tcatataagt aaacaagttg
     3001 agaaagcagc atcaggagga aatgtgatag ctccaagtaa taggaaaagc actccaagtc
     3061 ctgaatttag tactttttca gctcaaagaa caagctcatt tgtaaatgca agttcaagtg
     3121 atatagaata ttatttaaat cctaaaaatt ttacaaatac tactaaaggt atgatgcagt
     3181 ttttaaagat taacagttat agagatggta tttcagaatc aagtcttaac tcgtatctta
     3241 atggtttatc ttctagtgta tttaaaaacc agggagcagc ctttataaat gctgccaaga
     3301 agtataatat agatgttgta tatctagtat ctcatgctat gtgggaaact gcttatggta
     3361 agtctacact tgcccaaggt caaacattaa catcttataa gggacaacct cttagtaagc
     3421 cagttaaagt atacaatttt tttggtatag gagcaataga taaaagtgct aatgtttcag
     3481 gtgcagaagc agcgtattcc aatggctgga ctagtgtaga agctacaata gatggctctg
     3541 caaagtggat atcacaaaat tacgtaaata gttctaagta caatcagaat actatctaca
     3601 agatgaagtg gaattatgat tatacatggc atcagtatgc aactgatgta aactgggcta
     3661 atggaatttc tggaattatg gaaaatttaa ttggtcttta tggaggagga agtagtttgg
     3721 tttttgaagt tcctcaatat aagtaataca taaataaaaa atatagacta tgggtagttt
     3781 tcattttatt taaattatct atagtctttt tgtatataat ttataatctg taaatttaat
     3841 gttaacaaaa cttttatgtt ataatattaa ttctaagtaa atataagtga gtaataggta
     3901 gaaatagata aaaagtggta ataatatata taaagataat tttgagaaag gaggatatat
     3961 atggaaagta ttaaggaata tttggataag ttagaaatta ataatagtgg tcttggaaag
     4021 caacttaaag aagtttatat aaatagagta gtttatttta aggaagataa gatagtttat
     4081 ttttatctaa cttctaaaga tattgtttct catgaacttt tagataaatt taaagaagaa
     4141 ttgatgtata aactcgatta ttttaaagat atgaggatga agataagatt tactggtctt
     4201 gaaagaaaat ccaataaaga tgttataaaa aaatattgga ataatatatt gtatatatta
     4261 aagtatcttt gtccatctat tgcagggtgg tataaacaag tagaatttct ttgtttagaa
     4321 gaagaattaa aaataaaact tccaaaaggt atattttatg agcgattaat gaaaaaaaat
     4381 gtaacttatg tacttaaaac cgtgcttagc gaagagttgg gattagattt aaatataaca
     4441 atagaaaaag ctgttgatga aaaggtaaat aaagaaagac tcataagaat caatgataga
     4501 gaaatggaag aaaaaattag ggctttagaa attggaaaag ttaataactg tgaagaaaat
     4561 gaagaggaaa gctatgtcat taagtctgat gtagatgaaa atttaatcta tggagataat
     4621 gcaaatgcta tgattgaaaa tatagttgaa ttaaatgcat cttctggaac agttgcagtt
     4681 gttggagata tatttgatgt tgacactaaa gaacttaaaa acggaaaaat acttatgata
     4741 gcttcaatta cagattatac tagctcaata agctgtaaac tttttttaac agatacaaat
     4801 aaggatggag tacttgaaaa tgttaaaaag ggagcatact taaaaatcaa gggtgacata
     4861 gtttatgata catatcaaag agaaattagt atgatgatta gtgggataag aaaagaaact
     4921 agaatagaga gagaagataa atcagaggaa aaaagggtag aactccatgc acatacacaa
     4981 atgtcttcta tggatgctat ttgttctgtt aaaaagcttg tagagcgtgc agccaaatgg
     5041 ggtcattctg ctattgctat aacagaccat ggtgttgtac aaggatttcc agatgctatg
     5101 aatgcaggta agtcaaataa tataaaaata ctttatggag tagaagggta cttggttgaa
     5161 gatgattctc tcattataga ggatgcaaat gataaagaac tttcgcagac atttgttgta
     5221 tttgacatag agacaactgg tttttctaat acaaatgata aaataactga gattggtgct
     5281 gtaaaaatag aaaactttaa gattgtagat agatttagtg agttaataaa tcctgaaaaa
     5341 gatatttcct ataagataca ggaattgaca ggaataacta atgaattaat taaagacaag
     5401 ccaaccattg aagaaattct tcctaagttt atggaatttg ttggagatag tgtattagta
     5461 gcacataatg cagagtttga taccggattt atatcgcaaa aatgtaggga acaaggcttg
     5521 ttatataata acaaaaaagt agatacactt atgttagcaa gagtaatgtt gccaaactta
     5581 aaaagacata gattaaatgt cgttgcaaaa gaacttggta ttcctctttt aaatcaccat
     5641 agagcagtgg atgatgctga ggctacagca cttatattta ataagttttt gcagatgctt
     5701 acagaaaaag gtgcaaaaac tctaagtgat gtaaacaata tacttggaaa gattgattac
     5761 acaaagctta gcacaaatca tataaccttg atagcaaaaa attctatagg tataaaaaat
     5821 ttatataaaa taatttcaga tgcacatgta aatcattttt ttagagcacc aagaatcctt
     5881 agaagtgtat taaatgaata taaagaagga ttaataattg gttcggcttg tgaagctggg
     5941 gtggtttttc aagctgtaaa gaaaaacgtg tctgatgagg aaatgaaaaa aataatagat
     6001 ttatacgatt atattgaagt aatgcctata gataataata gatttatgat tgataaagga
     6061 gaagtaaagg atgaagaaga gttaagggag ttgaatagga aattaattga tacagccaaa
     6121 aaatttgaca aaattccagt agctactggg gatgttcatt ttttagacaa acatgaagct
     6181 gtcttaagaa aagttcttaa atattctcaa ggttttaaag tagatgaaga agaaacttat
     6241 ttacatttta gaacaacaga tgaaatgttg gaagagttta gttatcttgg agaagaattg
     6301 gcatatgaag ttgtagttga aaatagtaat ctgattgccg atatggttga agatataaaa
     6361 cctataccag atgatactta tcctccaatt atagagggct cagatgtgga gttaagagaa
     6421 atgtgttaca aaaaagccaa acgaatctat ggagacccaa taccagaaat agttcaaaaa
     6481 agactggata gagaattaaa ttcaataata agcaatgggt atgcagttat gtatattata
     6541 gctcaaaaac tggttgcaaa atctctttca gatggatatt tagttggttc aagaggctca
     6601 gttggctcat cttttgcagc tactatgagt gatataacag aagttaatcc tcttccagca
     6661 cattatattt gtgaaaacga agactgtaaa tattcattct tttatgaaat aggtgaatgg
     6721 ggctcaggtg ttgatttacc agacaaggat tgtccaaagt gtggaagaaa acttgctaaa
     6781 aacggtcatg atattccatt tgaggttttc ttaggttttg aaggtgataa ggaaccagat
     6841 atagacttaa atttctcagg aaattatcag ccaacaattc ataaatacac agaagaacta
     6901 tttggagagg ggtatgtata tagggctggg actataggta ctgtagcaga aaaaacagct
     6961 tttggatacg caagaaaata tgtggaagaa aataatataa gtgtaccaaa tgcagaagta
     7021 cttaggcttt caaatggatg tactggagta aaaagaacct caggacaaca tcctggtgga
     7081 gttatggtta taccagacta taaagatgta tacgatttta cacctataca gtatccagca
     7141 aatgatactt cttgtggtgt tataactact cactttgatt atcattcaat aagtggtaga
     7201 atacttaaac ttgatatact tggacacgac ggtcctacca ttataaggat gcttgaagat
     7261 atgactggaa taaatataac agaaattcct ttagatgata aagagactat gtctctattt
     7321 acatctacag aagcattagg tgttacacca gaagaaataa actgtcctat aggttgcctt
     7381 gctgtaccag agtttggaac taagtttgta cgacagatgt tacttgacac caaacccact
     7441 acatttgcag aacttgtgcg tatatctggt ctctctcatg ggacagatgt ttgggtaaat
     7501 aatgcacaag acttagttag agaagatata gttggattaa aagatgttat atctacacga
     7561 gatgacataa tgaactattt aatatttaaa ggattacctc ccaaaatgag ttttacaata
     7621 atggagagcg taagaaaagg taaaggtcta aaaccagaac acattgaaga aatgaaaaaa
     7681 aataatgttc cagaatggta tataggctca tgtaaaaaga ttaagtacat gttcccaaaa
     7741 gcacatgctg ttgcttatgt aatgacatca tttagaattg catattgtaa agttcactat
     7801 ccagaagcat tttatgcgac ttattttacg acaaaagttg aggattttga tgcagattta
     7861 attgttaaag gattagatgc tataaaatca aaaataaatg aaattgagtc actgggaaat
     7921 gatgcaacca caaaagaaaa aggtatgttg accgttttag aagttgctct tgaaatgtat
     7981 gctagaggaa taaaattatt accagttgat atatataaat cagatgcaac tgagtttata
     8041 gttgtaggag aaaaaacatt acttccacca atggcagcaa tacaagggct tggaggaaat
     8101 gcggctataa acatccaaaa tgaaagaaaa aatggtgaat ttatatcaaa agaagatttg
     8161 agaaagagaa caaaaatatc aaaaacggta atagaaactc ttactaatca tggttcactt
     8221 gagaatatga gtgagagaaa tcaattgtca ttgttttaag ttagcccaaa aagttgcaaa
     8281 taaaagaatt ttatgatatc atagtataga aaatagtata ctttgcagaa actaagagtg
     8341 agacagctgt tctcactctt ttattttaaa tatattatta aaaagttggt ttatttttat
     8401 taaatatatt ttatttaaga ttatataaac taatcattta caattgaata ggaggaactt
     8461 taatgaaaaa aaatttagaa gctacaattg aggagattgt aactaaaata acggatgaac
     8521 atggatttga aatggttgat gttgaatatg taaaagaagc aggagaatac tacttaagag
     8581 tatacataga taaagaagaa ggaatatctt taaatgaatg tgaattagtt agtagagaat
     8641 taagtccaat actagatgaa aaagacccta taaaagaaaa ttacttttta gaggtatctt
     8701 ctccaggact tgatagagca ttaaaaaaag acagagattt tgttagatat caaggtagag
     8761 atgtagattt aaaattatat aaaccattaa atggatgtaa gcaatttgaa ggtgaacttg
     8821 taggattaac agaagataat aatataaaaa tcattgcaaa tggtaaggaa atggaattta
     8881 atagaaaaga tgttgctatt gtcagattag caataaagtt ttagtggtat attaaatagg
     8941 gaggatataa aaaatgaatc atgaatttat ggaagcatta gatgaattag tagttgacag
     9001 aggtatagat aaagaaatac ttatagatac tatagaacaa gctcttttaa ctgcatacaa
     9061 gaaaaacttt ggttcagcac aaaatgttag agttgaattt gatagagaca agggagatat
     9121 aaaagtattt tctcaaagag ttgttgttga tgaatcagat ttatatgata ctttcttaga
     9181 aatagagtta gcggatgcta gagagataaa ccctaattat gaattaggtg atattataga
     9241 acatgaagtt acacctaaaa actttggaag aattgcagct caaacagcaa agcaagtagt
     9301 tgttcaaaga ataagggaag ctgagagaga aatagtgtat aatgaattta tagaaaaaga
     9361 aaatgaaata gttacaggag aggttgctag agcaaataaa aatgttgttt atgtaaatct
     9421 tggaagaata gaaggtataa tgactcaaag tgaacaaata ccatttgaga attataaagc
     9481 aggtcaaaaa ataaaattct atatacttga agttaaaaag actaacaaag gacctcaaat
     9541 agttgtatca agaagtcatc cgggtctagt aaagagattg tttgaagaag aagttcctga
     9601 aatatatgaa ggaatagttc aaataaaatc tgtagcaaga gaagcagggt caagaactaa
     9661 aatggcagta aaatctatag atgaaaaaat agacccaatt ggagcttgtg ttggaccaaa
     9721 aggacttaga gtaaaaaata tagtggatga acttggcgat gagaaaatag atataataaa
     9781 atatagtgaa gacccagcag agtttatatc agcagcactt agcccatcaa aagttgttag
     9841 agtagatata gatgaagatg aaaagtctgc cttagtaatc gttccagatt atcagttatc
     9901 gcttgctata ggtaaagaag gtcaaaatgc aagactagca gcaaaattga ctaactggaa
     9961 aatagatatt aagagcgaat ctcaagcaga acaagaaatg ttaaatcttg aaaattccaa
    10021 agaaatagaa gctgaggatg ttgaagctga atttatcgac caagaagagt taggtacttc
    10081 agaggataat tttagtgaag aagagtaagg agggatgatt ttgaaaaaag taaaaaaagt
    10141 gcctcaaaga aaatgcatag catgtcaaga tagagactct aaaaaagagc ttataagaat
    10201 agttaaaaat aaagaaggaa aaatattttt agacccaaca ggaagagcca atggaagagg
    10261 agcatatgta tgtaaaagct ctgaatgtct aaaaaaagct ataaagagta aagcattaaa
    10321 taaagctttt aaaatagatg ttccagatga agtctatgat aatctactta tggagttaga
    10381 agaatatgaa aaataataaa gaaaaagtac tttcgttctt agggctcgca actcgtgcag
    10441 ggaaaatagt gtctggtgat gattcaactt tgcttgacct taaaaaagga aaagtaaatt
    10501 tgattttaat cgcagaagat gcatcaaata atactaaaaa actttttaaa gataaatcaa
    10561 cttttagaaa tattccatat ctattttttt caacaaaaga ggaaatagga tttgcaatag
    10621 gaaaatctcc aagagcagtt gtaggaataa aagatgaaaa tttttctaaa aagataattg
    10681 aactaataga aatttaaaat aaaatagggg gtgatcttgt gtcaaaaaca agagtatacc
    10741 aaatagcaga agaattaaat atatcaaatg aagagcttat aaataagtta gctgaattag
    10801 atataaatgt aactgacaaa gattcagtat tagaaggtga agagttagaa ttagcacttg
    10861 aaatgttagg tgaagattta tctcaagaaa atggaaatgt tatagaaata gatggaaagt
    10921 tgacagtaca agtattagca acgaaattag ataaatctcc atcagaaata attatgaagc
    10981 ttatgaaaat gggaactatg gcaacaataa atcaagagat aagttttgag attgctgctt
    11041 tggctgccaa agactatgga tttgaattaa cagtagctga aagtgatgat acagaagctt
    11101 tagaaataga agctttaatg gaaattgaag aagataaaga agaggattta aaacctagac
    11161 caccagttgt tacagttatg ggacatgttg accatggtaa gacatcttta ctagatgcaa
    11221 taagaaaaac tgatgtgatt tcaggtgaag ctggaggaat aactcagcat ataggtgctt
    11281 cagaagttaa aatcaatgga cacaaaatag tatttttaga tactccagga catgaggctt
    11341 tcacatcaat gagagcaaga ggagcacaag ttacagatat agcaatatta gtagttgcag
    11401 cagatgatgg tataatgcct caaacagttg aagctataaa ccatgctaaa gcagcaggtg
    11461 tacctttaat agttgctatt aataaaatag ataaaccagg tgcaaaccca gataaggtta
    11521 aacaggaatt agcagaccaa ggattattag ttgaagactg gggtggagaa gtaatagcag
    11581 ttccagtatc agctaagaaa aaagaaggaa tagatacact tttagaaatg gtattattgg
    11641 ttgctgaaat ggaagagtta agagctaatc caaataaaag agcagtagga actgttatag
    11701 aagctgagct tgataaagga agagggccag ttgcaacagt acttgttcaa ggtggtactt
    11761 taacagtagg tgacccaatc gttgcaggtg ttgcatgtgg taaagttaga gcaatgataa
    11821 atgcaaaagg taagagagta aaaacagcag gaccatctac agcagttgag atattaggtc
    11881 tatcagaagt tccacaagga ggagaccaat ttgtcgaagt tccaactgat aagattgcta
    11941 gaagtgtagc agcaagaaga caacaaatag ttagagatga aatgttaaaa tcaactcaaa
    12001 gattatcact agatgctcta tttagtcaaa tgagtgaagg ttctataaaa gaccttaata
    12061 tagttataaa agcagatgta caaggttcag tacaggcagt taaacaatct ttagaaaaat
    12121 tatcaaatga agaagttcaa gttaaagtaa tacatggtgg tgttggagct gtaacagaat
    12181 cagacatatt acttgcagca gcttctaacg caataataat aggattcaat gtaagaccag
    12241 ttccaggtgc agaatctcta ggagaaaaag aaaatgttga tataagaaca tatactataa
    12301 tatacaaggc aatagaagat atacaagcgg ctatgactgg tatgttagac cctgaatatg
    12361 ttgatgaaga aacaggaaaa gctgaaataa gagaaatata taaaatatct ggtgttggaa
    12421 cagtagctgg atgttatgtg actaatggta agatatttag aaattgtaaa gtaagattag
    12481 ttagagatag cataataata catgaaggtg agcttgcagc acttaagaga tttaaagatg
    12541 atgtaaaaga agttaattct ggatacgaat gtggtatgag ttttgtaaat tataacgaca
    12601 taaaagaagg cgatatagta gaagcttata taactaaaga agtagaaaga aaattataaa
    12661 ttcgtatcaa agaaaatcag ttagtaaaga aggtcgatgg tatggcatca tataatagaa
    12721 caagaaggat agcagaagaa ataagaaaag tcgtaagtac tatgcttata aatggagtaa
    12781 aagaccctag aataacatct atggtgagtg taactgatgt agaagttaca aatgacctaa
    12841 gatatgctta tgtatatgta agtattctag gtggagatga agaatccaca ctaacaggtc
    12901 ttaaaagtgc tggaggatat ataagaagag aagtaggaaa aaatataaaa cttagatata
    12961 ttcctgagat agtatttaaa cttgatgatt caatagaaaa aggaatgtat atggataacc
    13021 ttataaaaag agttaatgaa aaaaatgctc aacaaaatga ggatgaaaat tatgatgaat
    13081 aatatggata gtattataaa ctatatttat gagggtgact attttatagt tacctctcat
    13141 atcagtccag atggagacaa tgttggttct acactttcaa tgtattatac attgaagaat
    13201 ttaggaaaaa atgtatatta tgtacttgat gatgaagctc cattaaattt aaggtttttg
    13261 gttgaaggtg taaaaatcta taagtctaat gaatttaata tgaagaacta ttctttaatt
    13321 gcacttgatt gtggggataa gatgagaata tgtgtgagtg atgaaatcaa aaataatgca
    13381 tcaaaattaa tctgtattga ccatcatgct agtaatgatt catatggaga tttaaattat
    13441 gtagatattg atgcatcttc tacatgtgaa ctagtttata atttattggt tagatttcag
    13501 caaacaaaag atattaatat tataaatgaa gatatagcaa catgtttata tacaggtctt
    13561 gtaactgata ctggaaactt ttcgtattca aatgtacatg caagtagttt tgaaatggca
    13621 aaaaatttac ttgttttagg tgcacaaaaa aatactataa tacaaaatat atatcaaagc
    13681 aattcttctg attattataa attattagga gaagctctaa aaggtttgga gatatttgat
    13741 tcaaaggtat ctagtatagt tttaactcaa gacatgatga atagaaataa tattagcttt
    13801 aatgatgttg atggaataac aacatataca agagatataa aaggtataga agttggaatt
    13861 ttatttaaag agaaaaaaca aaatgaaatt aaagtgagtt ttaggtcaaa aaattatgtt
    13921 gatgtaagtg aaatagccaa gttatttggc ggtggtggtc atgtaagggc tgctggatgt
    13981 actattagag atagtattga taatgcaaag aaaatggtac tagaggcagt gttaaaatca
    14041 atttaaggtg acagtaaaat gaataagatt ataagtatat tgaaacccac aggtatgaca
    14101 tctcatgatg ttgtaagtag agtgagaaag attttaaata taaaaaaagt tggtcataca
    14161 ggtactcttg acccagatgc ttcaggtgtt ttaccaatct gtatagggaa agctacaaaa
    14221 gtatgtgaag ttattttaaa caaagataaa tcgtatatat gtgagcttac tttaggtata
    14281 agtacagata cttatgatgc ttcaggtgaa attttaaaaa aagttgatga ttttaaattt
    14341 agtaatgaag acatagaaag agcttttgat acacaaagag gagagataaa tcaacttcct
    14401 ccaatttatt cagctttaaa ggtaaatgga aagagaatgt gtgatttagt aagaagtggt
    14461 agacaatctg aaattacttt aaaaacaaga agagtaaata taaaagacat aaaaatacta
    14521 agtataaaag gtaataaagt aatgttttat gttgagtgtt ctaaagggac ttatgtgaga
    14581 agtatttgcc atgatatagg agaatatctg ggttgtggag ctcatatgtc gtttttgaat
    14641 agaacatctt ctggtaaatt tgacttagat aattcaataa cattagaaga attagaatta
    14701 ttttatgaaa ataaaacttt agacaaatac ttatatgata tagattatgt ccttgattcg
    14761 tttaattatg ttgtattaaa ccctaatgct ataaaatatt atagtaatgg tggctcaata
    14821 gatgataaaa ggtttttaaa aaataatttc gataaagatg atgaagttgt aagagtatac
    14881 agtacagata atttcttagg attaggaaag ttatcaaaac acaataatac aataagtgtt
    14941 aaaagtgata agatgttcat ataaccaatg tatttaaaaa aacaacaaaa gagatagtac
    15001 tataataact tctatagtct atctctttta cttatgctat aatagtttgt gaataagtta
    15061 agagtaaatt atattttagc ggggaacatt tattatggtg attataaaat ctataaaaga
    15121 aatagcaaac ataaaagaaa gtgtcattac cataggaaat tttgatggtg tacacaaagg
    15181 acatcaagtt cttataggaa aaacagttga atatgcaaaa aaagaaaata tacagagtat
    15241 tgtatttaca tttgcaaatc atcctgtaaa ttactttaga ccaaactcaa caaaaaatat
    15301 tataagcaat gatgataaaa taaaattatt agaagatttg ggaatagata tagttgtaat
    15361 cataccattt gatgagtata tgactaaaat acctgcaaag gatttcgtag atgagatact
    15421 agtaaaaaaa ttaaaagcta aaaagatagt aattggacat gactttacat ttgcaagggc
    15481 aaaagaaggc aatgttaatc tattaaaaag cttagaacat gaatttggtt ttgaggtaga
    15541 agtaataaaa cccatcaaaa tcaatgatgt aagagtgagt agtacttata ttagaagttt
    15601 agtatcccag ggtgatatgg ctaatgtcaa ggaatatttg ggtagaaatt acaagttaga
    15661 aggttgtgtt atacattcta aacaccttgg aagaactata ggattcccaa cggcaaatat
    15721 tgaccttaag aataatatgc tcgttccaaa gagaggtatt tatgcttcta ttgtacatat
    15781 tggagatgag acttattttg gtgctacaaa tgtaggctat aacccaacag ttaatggaaa
    15841 aactttgtct attgaaacaa atattttaga gtttgataga gatatttatg gggaaaatat
    15901 aattgttgaa tttttggaaa gaataagaga tgaaagaaag tttaattcaa tagatgactt
    15961 aaaaaatcaa ttgtataatg atacaaatta tgtgtatgaa aactatgttt gcaaaaacaa
    16021 gtaatgtatg ataaaatgat taaggaaaat aaccattact cggcatttga atactccaat
    16081 caatgcttag taataggcgt ttctaaaaaa atacggaggt aatctaaatg ataaacaaat
    16141 cagaaataat aaaagaatat ggaagagctg aaggggatac tggttctcca gaagtacaaa
    16201 tagctttatt aacagctaga ataaacgagt taaatggaca tttaaaaact cataagaaag
    16261 accaccattc aagaagagga ctattaaaaa tggttggtag aagaagaaac ttattagctt
    16321 acttaaagga aaaagatctt gaaggatata gagcattaat agctaaatta ggattaagaa
    16381 agtaatttca ttctttataa aaaagcaggt atagtgtata cctgcttttt tatagttaat
    16441 tcattataaa gtaaaatagt taataataaa agtagtttat atgttataaa aaattataaa
    16501 atgaataata ttgggtaaga atattaaaat agtatacaaa tacataatca atttaaaagg
    16561 aaagacagga taaatataga aattttataa caggataatt agttggaata aggaggatat
    16621 tattttgaaa ataaagttag tatgcgatag tttgtgcgat attccagatg aaatatcaga
    16681 aaaagattat atagagatag ttccacttac agttattttt aatgataggg aatatataga
    16741 aggagtagat ataaagaaag aagagtttta taaaaaagta aaagaaatca agcaaatacc
    16801 taaaacttca caagctacat atatggagtt taaagaagtg tttgataagt tcataacaga
    16861 aggttatcat ataatctgta tgacaggggc tgcaaacgca tcaggaactt ttcaaagtgc
    16921 tatgatagct aaaaatgatg taaatgaaaa tgaaaaaata catatatttg atactagaaa
    16981 tttatctcta ggtagtggac aatatgttat aaaggcatgt gaacttcttg aagaaggact
    17041 gggatttgaa gaaattatag atgaattaga aaatactcgc agtagtgtaa gattactttt
    17101 tgcaccatat acacttgatt ttttaaagca gagtggaaga gttccagttg caactgcttt
    17161 gataggaaat atgttaaata taaagcctat atttttcttt gataatgggg aagctaagtt
    17221 ggtaaataag gttagaggaa taaagaatat agcaagtaaa ctggtcgata taattttaga
    17281 aatgaatgaa gggtctttag aaggaaaaat cgtcacaata ggttgtggag ataatttaca
    17341 cgattgtgaa atattaaaag atgaagtaaa taaaagaata aaagcaagaa gagtattatt
    17401 tactcgagga ggagtttcta tttgctctca tacagggcca gatatattgg ctataagttg
    17461 ttctaattag tagtaatata aggtttttgc atatatatgt gaagataatt ttatattgtt
    17521 aatttatagt atttttgata atatagtaaa tagagtgata tacgtgtttt ttaataagag
    17581 gaggacaagc attaatgttt gaacataaaa tatttaaaat ggattttgca ggaagagaac
    17641 tttctgtaga aataggaaag atttgtgaaa tggctagtgg tagttgtata gttcgataca
    17701 gtgacagtat ggtaatggta aatacaacta aatcagccaa acctagagac ggaatagact
    17761 tttttccttt aagtgtggat tatgaagaaa aattatactc tgtaggtaag atacctggag
    17821 gttttttaaa gagagaaggg aagccttcag aaaaagctat acttacatca agattgatag
    17881 atagaccaat aagaccttta ttcccaaaag gatttagaaa tgatgttcag gtagttgcaa
    17941 cagttctatc agtagaccaa gattgtacac cagatatagt agcaatgata ggttcttcaa
    18001 tagccctttc tatatcagac ataccattta atggaccaac aggttcagta tgtgttggcc
    18061 ttgtagatgg tgcatttgtt gtaaatccaa acgcagagca aagagaaaaa agttcaatgc
    18121 atctagttgt ttcaggtaca aaggaagcaa taatgatggt tgaagccgga gctgatgaag
    18181 ttccagatga ggtaatgctt gatgctattt tatttgctca ccaagaaata aaaaaaatag
    18241 ttgagtttat agaaggtata gttgcagaag ttggaaaaga aaaaatgcct gttgaattat
    18301 accatgcagg agaagaaata actcaattgg ttcgcgaatt tgcaactgat aagatgaaaa
    18361 aagcagttca gacttttgaa aagttagaga gaatggaaaa tatggataga gttaaagaag
    18421 aaactttagc tcactttgaa gaaactttag aagattttga agattttgtt ggagatatag
    18481 aagaagtatt acaagatata ataaaagaag aagtaagaaa acttatagta catgaaaatg
    18541 taagaccaga taatagaaaa cttgaagaaa taagacctat atggtgtgaa actggcatga
    18601 tacctagagc tcatggttca gccatattta ctagaggaca aactcaagta cttaatgttg
    18661 ctactttagg tgctttagga gatgttcaaa aattagatgg gttagatgaa gaagaaaaca
    18721 agagatatat gcatcattat aatttccccg catatagtgt tggagaagct agaccatcaa
    18781 gaggaccagg aagaagagaa ataggacatg gtgctttagc agaaagagct ttattaccag
    18841 taataccatc tcaagaagaa tttccttatg ctattagatt agtttctgaa gtattaagtt
    18901 caaatggttc tacatctcaa gcaagtgtat gtggctcaac attatcttta cttgatgcag
    18961 gtgtacctat aaaagatatg gttgcaggta tagcaatggg acttataaag catgatggta
    19021 aggttgcagt gctttcagat atacaaggta tggaagacca tttaggcgat atggacttta
    19081 aagtagctgg aacagagtat ggtataacag caatacaaat ggatataaaa attgatggta
    19141 tagataaaga aattctacaa cgtgctttaa aacaagctaa agagggtaga atccatatac
    19201 taggagagat gagaaaaact atatctcagc caaaaccaga actttcacca tatgcaccta
    19261 agattgttaa aatgcaaata aatcctgaca agataaaaga tgtaataggg cctggtggaa
    19321 agataataac taagataata gacgaaactg gagttaagat agatattgaa caaactgggg
    19381 aagtatttat atcaggtatt gagattgata tgattaagaa agctcaagaa cttataaata
    19441 atatagtagt tgaaccagaa gtaggaaaaa cttataaagg taaggtaagt agaataatga
    19501 actttggagc atttgttgaa atacttccag gaaaagaagg attacttcat atatctcata
    19561 tagctcatga aagagttgcc aaagttgaag atgtattgaa tataggggat gaagttgaag
    19621 ttaaagttac agagattgat gaaaaaggaa gagttaattt atcaagaaaa gttcttttac
    19681 caaaaccaga acataaaaat aaataactta ttatataatt aaaagcaaag tcttaggatt
    19741 ttgctttttt aaatacaaaa aagtagataa ttttaaaatg atagttatgg atggcttata
    19801 gttctttttc tgtaaggctt tactcttata aagaagctat ttaaaagctg attaatagaa
    19861 agctttattg atatatatag aatcacttaa gttatattta gtgcataaac agagcttatt
    19921 tttaatagta ttaaataaga aacaatttgt acataattag ggggagtttt aatgataatg
    19981 atggtactta ataagagtaa gctcaaaaaa atcataatac taattttagt agtgttatta
    20041 gtattatcag gaggggtaat ggtattaaag ttatttaaca acaagccagt atttaattta
    20101 tttaatggtg tagatttacc atatgaaaga ggtacaaagg ataaaagtgg atatgttgca
    20161 ttaacctgta atatagacct tggatgggaa acagagtacg tagaatccat tttagaaaca
    20221 cttaaaaaag aaaatgtaaa gattacattt aatgtaactg gaaaatgggc agaaaagaat
    20281 aaggatgaat tattaaagat aaaaaaacaa ggacatgaaa taggtaatca tggatataaa
    20341 catttagatt attctacatt atcatatgaa gacaattatg agcaaataga gacctctaaa
    20401 aagataattg aagaaattat aggtgaaaaa actaaatttt ttcaagcacc agcaggttcc
    20461 tttggtccag aaacagtaaa ggctgctaaa gcattagggt atacttctat caaatgggat
    20521 gccgatacta tagattggaa atacaaagac caaccagagg taatcattga tagaatgaag
    20581 aaaaaagaca ttaaagatag tagtataatc ttaatgcatc caaccaacgc aacgacaaaa
    20641 tgtatagatg atattatagc tattgtaaga gaaaaaggac taaaaccagg gaaacttagt
    20701 gatgtattta agtagacaaa gtataaatat ttggttaaga ttgtataagt tgtagttgaa
    20761 ttaattaaaa gactgactta taatgtaata catataaaaa ataaatataa ttaaaacata
    20821 gtgttttcaa taatccaata tgaaacaaag aataaattgt taggagaatg taatgtataa
    20881 gacaaaaata ctggaaaatg ggcttacaat aattggtgaa gaaataccat atctaaagtc
    20941 aataacatta ggtatttgga taaatgcagg ctctaggata gaagaagcac aggtgagtgg
    21001 aacttctcat tttatagagc atatgatgtt taaaggaacc aaaaacagaa catcaaaaga
    21061 gattgctagt tcaatagata atttgggtgg tcaaataaat gcatttacaa gtaaagaatg
    21121 tacatgttat tatgtaaaac taatagatga gcatatagat acgggaattg atgtattgag
    21181 tgatatgata ctaaattcta aatttgataa aaatgatata gataaggaaa gacttataat
    21241 attggaagaa cttaagatgt atgaagactc tccagatgac ttatcttatg atttattagt
    21301 ggaaaatata tatgctaatg atggattagg aatgaatata attgggacaa aagaatctct
    21361 atacaatata actagagaat ccatgttaga atacttaaat aagtattata ttccaaataa
    21421 cgctgttatt tctatagctg gaaactttaa ctttgatgat atggtggaga aaatcaaatc
    21481 aaaatttgga cactgggaaa agaaaaattt gagtatagac ataagtgagg ctaagtttaa
    21541 tccttgcttt atatcaaaaa ataaagatac tgaacaggtg aacttagcta tctgcctaaa
    21601 aggtattcct tttgaaaatg atgaagaagt ctactcaatg gcagtggtaa ataatatttt
    21661 cggtggaagt ataagctcaa ggctttttca gaaaataagg gaagaaaaag gtttagtata
    21721 ttcaatatac tcatcacaga cattatatag gaaatgtggt gaattaggca tatttgcaag
    21781 tatgagtaca gaaaatttac aagatgtata taatttaata aagaaagaaa ttgaaaatat
    21841 aagagaaaac tatcttacag aaaaagaaat atctgaaagc aaggaacagt taaaaggaaa
    21901 ttatatattg gatttagaaa gtactagcag tagaatgatg tctactggta aatctatgtt
    21961 acttagcaaa aaagtaaaaa ctacagatga aatacttgaa tgtataaata atgttaatat
    22021 taattcaata aagaaggttg ttgataaggt attcaatatc gaaaatattg gtacatgtat
    22081 agttggaaga gatgtagaaa agattctaca cttggattaa tattgaaaaa agtaaatctc
    22141 tggaggtata aaatatgaat ttatctgaaa tagcaggtaa ggaaatagtt aacttggtca
    22201 caggagaaag attgggggtc attggagaat gtgaccttat actagatgaa actacaggcg
    22261 gtatattagc gcttctaatt ccaaaggaaa aaggattttt tgccagaaga aaagataagt
    22321 catttttaga agtaccatgg agaaatgtaa aaaaaatagg taatgacatg ataattatag
    22381 aatatgaggg atatatttag gaggaaaaaa tgagtatttt agtgcaaaag tttggtggga
    22441 catcagtaga atcatatgaa aaaatgaatg aagtatgtaa gatagtaaaa gcatacaaaa
    22501 aaaatgatga agaattacaa ttagtattag tagtttcagc aatgggaaga aaaggagctc
    22561 catatgctac agatacactt ataaatttgt gtagtgcagt aaatgatgag ccaagtaaaa
    22621 gagaacttga tttaataatg tcttgtggag aaataatatc aggaacaatt cttgctaatt
    22681 tattgaatgc acaggggata gattctgttt tctttactgg tcagcaagca ggtataatta
    22741 caagtgatga atattctaat gcaaagataa agtatataaa tcctaaaaag gtaaaaagag
    22801 ctttagatga tggtaaggtt gtagttatag ctggattcca gggtgtaaca gatgatggag
    22861 aaataactac tcttggaaga ggaggaagcg atacatcagc agttgctatt ggtaaagctt
    22921 tagaatgtga aacagttgaa atttacactg atgtggatgg tataatgact gcagacccta
    22981 gagtagaacc aaacgctaaa gtattgagtt ttatagatta tgaggaagta ttccaaatgg
    23041 ctgacaaagg tgcaaaagta atacatccaa gagcagtaca gttggctaaa agtggaaata
    23101 taactttagc cataaagaat acaatgaatc caacttttga aggtacaaaa ataggttctt
    23161 tatgtagaca cttagaagag aatatagaat atgaacaaga aagagatttt aaggttgctg
    23221 ttgcaaataa agatagtgta gcacaagtta agattaaatc agcagaagaa gtgtttacag
    23281 aagtattaaa tgagatagaa aagaaactta taactataga tatgataaat ttctttatat
    23341 cagaaaaagc atttgttgta gaagatgctg atataaaatc attaaaggaa atactagata
    23401 aatttgaatt agattatgaa gtaaatagag actgtgctaa ggtaacattg atatgttcaa
    23461 gaattgctga tgaaatgtct ggtatcatgt caaaggtagt tagaggatta tcaaaagcag
    23521 gggtatctct tttacaaaca tcagactcaa atatgactat atcttgttta gttagtgaag
    23581 aagatatgca tacagcagta catgctatac accaacaatt ttatttaaaa taaactgtat
    23641 aatacaatta ttttttaatc aaatttaaga atttcatata tatcttaata tatttgattt
    23701 atttggtaaa actaaattaa atataatttt gataggagtt tttttatgaa agattataga
    23761 gaagataatg aaagatttga taaagataat tgtagtgaag aatctagttg cgaagagaga
    23821 gaatgtataa agcaatttgg taccaatgaa atgccacctc aacctcctaa agatattcaa
    23881 tgtataacta taattggaga aattgaggga cattttatag gaaatcctca aaaaaaagct
    23941 acaaagtacg agcatataat tccaatgcta tactcaattg aggaaagcaa tgatgttaaa
    24001 ggagtgttag ttgttttaaa cactgttgga ggagatattg aagcaggttt agctattgca
    24061 gaacttttaa acagtacatc taaaaaagta gttactcttg ttctaggagg aagtcatagt
    24121 ataggtgttc ctcttgctac agcaggagat tactcattta tagcaccaac agcaaccatg
    24181 attatacatc cagtgcgaac tactggtctt gtgattggta taaatgagac ttttgaatat
    24241 tttaaaaaga tgcaagatag aataattcaa tttataataa gaacatcaaa tatcaaaaaa
    24301 gatgtacttg aaaaacttat gcatgaaaaa gatgaacttg taagtgatgt tggtagtgta
    24361 ttaataggaa aagaagctgt ggattatggt cttatagatg aagttggagg actaaaagaa
    24421 gcacttaaaa aattaagaga gcttataaaa gaatctgagg aagaaaaaaa taatgattaa
    24481 aatttgttta aataataaaa tttatgatat aatgatgtaa taaagtagga gtgttagtgt
    24541 taaggcatta gcactttttg ctgtgaaggg gtaaaacttg ctaaaaataa ggagtgatat
    24601 taatggccac gaaaaagaag aaaaaaaaga aggtaaatac cttaagtttt aatgcagaat
    24661 atcataattt aataacaata tttataggtg tatttttatt atatagttta aattctaatt
    24721 ctatgggatg gatacctgtg ttaatgcaaa atctttttaa gggactattt ggagggcttt
    24781 caatagcaat accatttata gtaattataa caggactttt aggatttttt gatggaaatg
    24841 aatatatata taggctaaga aagactaaac tatactacat aattatttta tttatatttg
    24901 tattttatgg attattaaat gcaggaactc ttcctgtaga tagcccacta aaaggtaaca
    24961 tgtttgatga tgttatgaaa ttgggtgtaa gtggacaagg tagtggtttg atagcaacaa
    25021 ctattgcgta ttatatgagt aaaatatttg gtatagcagg tggatggtta ataagtatct
    25081 tcgcacttat actttctgta atgtttatat ttaatatttc tataaaagat ttggtgtcaa
    25141 atgcaaaatc aaaatctaaa gcatctaaag acagcaattt aacgtttaaa gataaaattg
    25201 caaatatgaa aaaatctgca atagatatga tgactgatga agtagatgat actactataa
    25261 ataaaaaacc aggtttcttt aaagggttaa tgtcaaaagg tcttaataat gatgatgaag
    25321 aagatgaata ttttgaagaa gaaaatagtg atggtgtaga tgataaaact ataaaaatag
    25381 ttggatttaa taaggcagaa gatgagtatt tagaaatatt agaaggaact caaagtatgc
    25441 cagagttaga tgttttaaaa gaattacaaa aagctaccaa tgaaaatcca gttgtagata
    25501 ctaaaccaga aaaaaaagtt gatatagcta aatctaattt aaatatagaa aaaactcaac
    25561 ctatgagtat tgtagcagag ccagttaatg aggattattc aaactataaa aagcctagca
    25621 ttgagctttt aaataaagta aataagaagt ctgatgaaaa tggtaaaaag aaagtgttga
    25681 aaaatgcttc tcttttagaa aagacactat cagattttgg agtggaagca aaaattaatc
    25741 aagttacagt tggacctact ataacaagat atgaaataca accaagtcca ggagtcaagg
    25801 taagtaaaat tgtaaattta actgatgata tagctttaag tttagcagct aaaagtataa
    25861 gaattgaagc cccaattcct ggtaaaagtg caattggaat agaagttcca aatgaagaag
    25921 ctcaaatggt tggtgtgaga gaagttctag aaagtgaaga atttaataat tttaactctc
    25981 ctcttgcaat gggacttgga aaagacgtag caggaaaaat tataattgga gatataggta
    26041 agatgccaca cttactaata gctggttcta caggttcagg aaagagtgtg tgtgtaaata
    26101 cattaataag tagtatttta tataaggcaa atcctgatga agtaaaatta ctattaatag
    26161 acccaaaagt agttgaactt gcaaattata atggaatacc acatctattg ataccagtag
    26221 ttactgaccc taaaaaagct gcaaatgctt taaattgggc agttacagag atgaatagaa
    26281 ggtataaatt atttgcagat gctcaagtaa aagatgtaac aagttataat gagaaagcgg
    26341 aagaaaaact tcctaaaata gtaataataa tagatgagtt agcagattta atgatggcaa
    26401 gtgcaaatga tgtagaagat tatatttgta gattagctca aatggcaaga gctgctggaa
    26461 tgcacttgat tgtagcaact caaagaccat ctgttgacgt tataacaggg gtaataaagg
    26521 ctaatatacc atctagaata gcatttgcag tatcatctca aactgattca agaacaatcc
    26581 ttgatatggg tggtgcagag aagcttctag gtaaaggtga tatgttattc tatccacttg
    26641 gtgcagcaaa accagttaga cttcaagggg cattcatatc agagagtgag tcggaaaaag
    26701 taattgattt tgtaaaaagt caggttaaag atggaataaa atatgaagaa gatataattg
    26761 aaactatatc taaggttaat acttcaaaga gtagtgatga ggatgagttt ttgagtgaag
    26821 cgatagaatt tgtcgtggaa agtggtcaag catctgcctc tatgctacag agaagattta
    26881 aaataggatt caatagagca gctagactta tagattctat ggaagaaaga ggcataattg
    26941 gagcaagtga gggaagtaaa cctagaaagg tattaataag caaacaggat ttgcagaatt
    27001 tagaaggtga atagaattgt cagttataaa aatagaaaaa gcacttaagc tagataaagt
    27061 aatatttgta gatgtgagaa ctgaaggaga atatgaagaa gaccatatat tgaatgcaat
    27121 caatatgcct ctttttaaaa ataatgaaca taatgaagtt ggaacaattt ataagatgca
    27181 gggaaaacat gaagcaatac aaaaagggtt tgactatgta tcgtataaat taaaggatat
    27241 atatttacaa gcagcagaac ttgcattaaa ttatgataat attgtcatat attgtgcaag
    27301 aggcggaatg agaagtggaa gtatagtaaa tttattatca tcattaggtg taaatgtata
    27361 tcaacttgaa ggtggatata aggcttatag aaattttgta ttagagtatc taaggcatgt
    27421 aatggataca aaaaactttg tatctgttca tggattgact ggtacaggaa agactgattt
    27481 gttacattta ttagatgaaa aaggaataga tgtactggat ttagaaggca tagctaaaaa
    27541 cagtggttct acctttggtt ttataacttt tgataaaaag ccaccatctc aaaagaactt
    27601 tgaaacaaaa ctatttgaaa gtgtattttt ttcgaaagag aattatatat ttgtcgagag
    27661 tgaaagcaaa cgagttggaa gtgttacagt tccaaatgaa attttcgagg caatgacacg
    27721 agaaggacat catatattat tagaatgtga tattgaaaat agagttgata gactttgtag
    27781 agattatatt tatgataaag atgaaggtaa tatattgatt ttaaaagaat gtataaacaa
    27841 gtttagaaaa agattgggta ataaagtagt agatgattat attgatttat tagaatcagg
    27901 tgaatataaa gaacttgtaa aaagatatct tttagaatac tacgacccac tttatatgca
    27961 ttcagtagag aaatataagt atagtaagat tataaatttt aatgatacac aaaaagcttt
    28021 agaagatgtt atagacttat atgagagtat attggaagga gaaagtgaat gttaaaaata
    28081 gcattggaat cattaggatg ctcaaaaaat ttagtagatg cagaaataat gatgggaatt
    28141 ttaaataaca aaggctataa attaattgga gattttgaag aagctgatgt aattatagta
    28201 aatacatgtg ggtttataga gtcagctaaa caagaatcta tagatacgat aataaacttt
    28261 tcagagttaa aaaaaactgg caatttaaag ttgttgatag taacagggtg tctagcccaa
    28321 agatattctg aagaattaaa aactgaaatt cctgaaatag atgctatagt aggaacagga
    28381 agctatcaaa atatagataa aatacttaaa gaattaagtg aaatgcatca aattgtaagc
    28441 cttaatgata tagagtttgt atttaatgaa gatttaccaa gatatatatc tacacctagc
    28501 tatatggcct atttaaaaat tggagaagga tgctccaata attgtactta ttgtataata
    28561 ccaaaactaa gaggtaaata tagaagtaga aaatttgaag atataattaa agaagctaag
    28621 aaattagctg aatctggagt aaaagaactt gtggttatag cacaagatac gacaaagtat
    28681 ggttttgatt tatatggaaa agaaagatta tcagaacttt tagaagaact tgcaaaaata
    28741 gatggattta agtggataag ggttatgtat tcatatccag aatctataac agaggaattg
    28801 atacaagtta taaagaaata tgataatata tgtagttact ttgatatgcc aatacagcat
    28861 gcaagtaaca atattttaaa attaatgaat agaaaaacta ctaaagaaga tatattaaat
    28921 aaaatcaatc tgataagaag taatatacca gatgctatac ttagaactac gataatagta
    28981 gggtttcctg gtgaaacaga agatgacttc aaacaacttg tggactttgt tgaagaggtt
    29041 aaatttgata gacttggtgc atttgcatat tcaagagaag aagatacacc agcagataga
    29101 ttgccaaacc atatagatga agaagtaaaa atacaaagaa gagatacttt aatgatgatt
    29161 caacaaaaaa tatctgaaga acttaatgac aaaaagatag gaaaaactta tgaggtgtta
    29221 atagaagaac agatagaaga taatgtatat acaggaagaa cacaaggtga tgcggaagaa
    29281 attgacagta tagtgtatgt aaaaagtgta gacaatcttg aagtaggaga atttgtaagt
    29341 gttcaaataa atgatgcaat ggaatacgat ttaatgggag atgttttata tgaacttgcc
    29401 taacaagtta actttgttta gaatattttt aataccagta tttgttttaa ttatgttgct
    29461 caatgtacca aataaatttt taatagcttg tattattttt ataatagctt caattactga
    29521 tgctttagat ggaaaaatag ctagaaaata caatctagtc actgactttg gaaaatttat
    29581 ggacccatta gcagataagt tattggttat ttcagcactt acatgtatga tagaagacca
    29641 tcttgtaagt agttggatgg taattataat agtggctaga gagcttacag ttagcatatt
    29701 aagagctata gctgctgctg atggaaaagt aattgctgct ggaaacagtg gaaaacttaa
    29761 aacgataact caaatggtat ctatagtgtt tttattgtta ggcgctcagt ttgaaaatgt
    29821 attgatatta aatattggtg aaattctcat tcttatagct acactactta cattatattc
    29881 tggatgggaa tatctatata aaaataaaga gctttttatg tcttctaagt aaaaaattat
    29941 gctttaataa ataaaaacta atgattaata aataaaaaat aacaacttaa aattttaaaa
    30001 atgta
//
LOCUS       DACTGW010000024        29740 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.024, whole genome shotgun sequence.
ACCESSION   DACTGW010000024 DACTGW010000000
VERSION     DACTGW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.024"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            45..926
                     /locus_tag="I9573_000842"
     CDS             45..926
                     /locus_tag="I9573_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HAT4795476.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKKGVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(1239..2018)
                     /gene="hisJ"
                     /locus_tag="I9573_000843"
     CDS             complement(1239..2018)
                     /gene="hisJ"
                     /locus_tag="I9573_000843"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HAT4795477.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEDLIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(2558..2908)
                     /locus_tag="I9573_000844"
     CDS             complement(2558..2908)
                     /locus_tag="I9573_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HAT4795478.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(2924..3340)
                     /locus_tag="I9573_000845"
     CDS             complement(2924..3340)
                     /locus_tag="I9573_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HAT4795479.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(3359..3550)
                     /locus_tag="I9573_000846"
     CDS             complement(3359..3550)
                     /locus_tag="I9573_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HAT4795480.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(3701..4516)
                     /locus_tag="I9573_000847"
     CDS             complement(3701..4516)
                     /locus_tag="I9573_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAT4795481.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(4563..5501)
                     /locus_tag="I9573_000848"
     CDS             complement(4563..5501)
                     /locus_tag="I9573_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT4795482.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDASNKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYIYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(5498..6370)
                     /locus_tag="I9573_000849"
     CDS             complement(5498..6370)
                     /locus_tag="I9573_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT4795483.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEK
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(6395..8323)
                     /locus_tag="I9573_000850"
     CDS             complement(6395..8323)
                     /locus_tag="I9573_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HAT4795484.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNSL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(8435..8812)
                     /gene="gcvH"
                     /locus_tag="I9573_000851"
     CDS             complement(8435..8812)
                     /gene="gcvH"
                     /locus_tag="I9573_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAT4795485.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(8899..11025)
                     /gene="cdhC"
                     /locus_tag="I9573_000852"
     CDS             complement(8899..11025)
                     /gene="cdhC"
                     /locus_tag="I9573_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HAT4795486.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(11082..11888)
                     /locus_tag="I9573_000853"
     CDS             complement(11082..11888)
                     /locus_tag="I9573_000853"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAT4795487.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(11938..13305)
                     /locus_tag="I9573_000854"
     CDS             complement(11938..13305)
                     /locus_tag="I9573_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HAT4795488.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(13336..14280)
                     /locus_tag="I9573_000855"
     CDS             complement(13336..14280)
                     /locus_tag="I9573_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HAT4795489.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(14300..15070)
                     /locus_tag="I9573_000856"
     CDS             complement(14300..15070)
                     /locus_tag="I9573_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4795490.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(15126..16511)
                     /gene="lpdA"
                     /locus_tag="I9573_000857"
     CDS             complement(15126..16511)
                     /gene="lpdA"
                     /locus_tag="I9573_000857"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAT4795491.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVIEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(16555..17436)
                     /locus_tag="I9573_000858"
     CDS             complement(16555..17436)
                     /locus_tag="I9573_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydrofolate reductase"
                     /protein_id="HAT4795492.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMQKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(17472..18146)
                     /locus_tag="I9573_000859"
     CDS             complement(17472..18146)
                     /locus_tag="I9573_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydrofolate reductase"
                     /protein_id="HAT4795493.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(18183..19055)
                     /locus_tag="I9573_000860"
     CDS             complement(18183..19055)
                     /locus_tag="I9573_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HAT4795494.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(19147..19779)
                     /locus_tag="I9573_000861"
     CDS             complement(19147..19779)
                     /locus_tag="I9573_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HAT4795495.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKIEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(19884..21560)
                     /locus_tag="I9573_000862"
     CDS             complement(19884..21560)
                     /locus_tag="I9573_000862"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAT4795496.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(21809..22588)
                     /locus_tag="I9573_000863"
     CDS             complement(21809..22588)
                     /locus_tag="I9573_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4795497.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(22898..24817)
                     /gene="cooS"
                     /locus_tag="I9573_000864"
     CDS             complement(22898..24817)
                     /gene="cooS"
                     /locus_tag="I9573_000864"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HAT4795498.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(25424..26335)
                     /locus_tag="I9573_000865"
     CDS             complement(25424..26335)
                     /locus_tag="I9573_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HAT4795499.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(26451..26573)
                     /locus_tag="I9573_000866"
                     /pseudo
     CDS             complement(26451..26573)
                     /locus_tag="I9573_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432270.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            complement(26656..27798)
                     /locus_tag="I9573_000867"
     CDS             complement(26656..27798)
                     /locus_tag="I9573_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAT4795500.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYKVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(27816..29570)
                     /locus_tag="I9573_000868"
     CDS             complement(27816..29570)
                     /locus_tag="I9573_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HAT4795501.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMKINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
BASE COUNT         8967 a         5973 c         3319 g        11481 t
ORIGIN      
        1 ctataaatat gtcgacacaa taaatttaaa aaggagaact aaatatgaaa aaaagattaa
       61 taataatgat attgtctgtt gtcttagtac tatcaagtat acttacgata tttgcatatt
      121 caaatattaa atataataat aataaattga tatattctaa tatgatagat aaaaaaactc
      181 aaaatagtgt aaaggaaatc ctaaaagaaa ataaaattaa tgaaaaagac atagatacat
      241 ttattaaagc agtcaacaat tataataaat tgcaagttaa aatacttcaa aataatataa
      301 atatatctaa atctggatat tcttcaatca atgcaaaaca agttccatat aacctagaaa
      361 aattgcaaga taattgggtg aaaaaattcc ctgattatat ggatgtaaat tgtagaataa
      421 ctgcttttag actttttaaa gattttataa attcaaataa gaaatttact ggagactcta
      481 ttgatttgaa tgtcgactta gacactataa tgaataataa agatgctaaa tttagcacca
      541 aagatgtaga aaaatttatc aacttctttt ctgcaatacc agctaaagat acagatgata
      601 caatcaaaat tgcagaacaa ataaaaaatg aatggaaaaa aagaaaaata tcttttaaaa
      661 ataataaaaa tatatctatt ataaatggat ttcttcgtta tccagaaact aaaaacgtat
      721 ttataggtca tacaggcata tgtattaaaa ctaaaaatgg cattctattt ttagaaaaat
      781 atggtgttac atcaccttat caagttacta aatttaaaaa taaaaaaggt gtaaaaaatt
      841 atatgtttaa tagattgaag atgtcagaag gtgaaattga attgcctgac cctataataa
      901 tggaaaacga taaacttatg aaataataac tttaaacgca aaatagtatt tataattact
      961 aagtcaatat aatagagaag gtctatagat ttgtgtaaat acaatcttat ctacaatctt
     1021 atataagaat gtttatataa ataagataga ttaattctaa gtcctattat tctattgaat
     1081 tttattatta ttttacttag attctattgt tattttactt agaacttatt gttattttat
     1141 ttaagttcta ttactatttt caatcatata aatttacaaa agactgtaca taaatttatt
     1201 ttatacaaac ttatctacag tcttttgtgt gaatatatct aaataatatc gtatttattt
     1261 atttcctctg cacagtgaga atcagaacct aaaactaatg gaatatcgta tttttttacc
     1321 aactctaata agtatccatc tatataaggc tctttgcaat actcttttct atttccagaa
     1381 acattataat caagttcata acctttttct ttaattaact ttattaagtc ttcaagaatt
     1441 gtatttcctt tataatcgta tggaaatacc tgattaaact ttctaactaa atttaaatga
     1501 cctattcttt ttggtttatg tatacctaaa tctgaattta cagcttttat caatgtttca
     1561 taatacttat tataaacagc ttcaatactt cctaatttgt cgattaaatt ttgaaactcc
     1621 tctacactgt aatctacaca ataataatca tcatcaattt taattatatg tactgataaa
     1681 agagagtctt ctaattcctt tccatattta tttaaattct cctttatccc ttcttcgtat
     1741 ccttctataa aatccacttc cgacccaaca tttatcttga ttttaccctc atacttttct
     1801 ttcaacttct tgacatcatt aaaatacttt ggtaaatctt caatactcat acaactatca
     1861 ttttcaggag cagggtctct aaatccattt gcatacggaa aatgttctgt aaatgtcatt
     1921 tctgttattc caacatccat tgctctttgt acatattttt caaaatcatc cttacttcca
     1981 tgaggacaat aaggtgaatg tatatgacca tctttcataa ataattcact ccttttttaa
     2041 agaaaaaatc taatataaat gcataaattt tttaacttca aaataatatt tttaatcatg
     2101 ctaattttta ttatatctaa tattagagta attttaaact gccttttaag tatcttatct
     2161 ttagtactat tttcaactta acattaatat tattttttaa cttaacatta atactatctt
     2221 taaatagtaa tactcaatat ataattaatc aaataactca aattgtttga tgaagctttt
     2281 gcagtattta aagaatatat aattatttaa cattaaataa tccaatcttg taataaaatt
     2341 tactcattaa agtatattac agaaaatatc tcaaaataaa ctttttagtt catgatatac
     2401 tcttttttgc atgaaattag atatattttt atcatttcaa caatgaaagg gaggcttaca
     2461 tccatcttga gataacccct tagataaaat ttttaataat taaacatcat aaaatataaa
     2521 aatcgcttat atcaaacgta taagcgatta attatattta catatgtttt ttagcctcta
     2581 acaacatttt attagcttct tccatatact ctatagcctt ttcaatactc tttgctgttt
     2641 cttcttttcc aatagctcta gccttttcaa cccactctct aaatccttct tcatgagttt
     2701 cattgtgatt tatccaatgt actaaaagta tttttaaagt tttttcatcc ttagactctg
     2761 atgattcctc atgactatgt tcatggtcgt ggtcgtgagc atggtcatgt ggatgttcat
     2821 gacatatatc tccatgagta tgttcgtggc agtggtcatg gtcatgatgt ccatgatgat
     2881 atcttacaat ctctttatca aaaatcatta gttattcccc ctgttatttt atattaaact
     2941 ctttttttat aaggtttaca gaatctttta caagtagtgc taatggttta ccttctaatc
     3001 caactaactc cacattttct ggcataatag gtaaaagtat cttaagtgcc tcactgtctg
     3061 caatagcttc acttatatta caagtaactt ctcccatcat agaattaggt attattacag
     3121 aaataggtgc aactattata tttgcttttt tagctgatac tactatagca ttttcccctg
     3181 tagctccttt attagcatga gctttcatca tagaactagt tgctatagaa ttagtcccta
     3241 gtgcataaat ttctacatat gtaggtaact cttctctcaa agaagaaact atctgggctc
     3301 caatacctcc acccattcca tctatcactg ctattatcat atgaaattcc cctattaatt
     3361 aattttcttg tattattatc ttatggtcca tcaatcttat ttctttaatt atcccatcta
     3421 ttatcttttg ctctcctaat aaatccgtta aataaatctt tccatcacaa gggtctatat
     3481 taaccacatt ttccataacc ttttctaact cattattact tttacaaatg aaagctgatg
     3541 attcacacat ataaatctcc tcctaatatg atttttatta aaaatcaaaa aaagattccc
     3601 ttaggtattt caatactcca aagaaacctt catgatttca gtttttgtat attattatac
     3661 cttaagtata tcactttaat ttatgtaaag tcaattaaag ttaacgcttt ttattatact
     3721 cttcattctt gtatttttca tatagttctt taactaattg cttctcataa tcagtcaaat
     3781 cgccctccac aaactcaaca ttaaattgtt cagctaatcc ctttactatg tttttctgta
     3841 gaatatctat gtctattttt ctgccaattt cattttctat tccacttgct tttttagctg
     3901 taaatttcat agctctttct ttcaattctg gtgtcttagt ttttattaat ttaaacaatt
     3961 tttctacatc aaaatccagt attattgaac catgttgtaa taaaacacca tcttttctgc
     4021 tttgagcact tccaattacc ttcttattat taatagtaac ttcatatgat gcatgtgcat
     4081 taaaacatgc agctgataaa ttttccctac ttattctctc gcctctattt aaattatctg
     4141 tttctatacc acttagattc aatcctttaa ctagaccttc acttatatat ctataagata
     4201 ggttgatgct cttatcgatt aaaggattat cttctcctat aattatacta taagttagct
     4261 cattatcatg taatacagct cttccacctg taattcttct agtatagtct atattcatac
     4321 atctacactt atctaaatct atctcatctt ccaatttctg aaaatatcct atactcaaac
     4381 aagctggttc ccatgtataa aatctaagag ttggcttatt atgtcctttc ttatatgcag
     4441 taaatatagc ttcgtcaatt gccatattca ttgctccttc atatgattta ttatgtataa
     4501 ctctccactg attcatgatt attacgcccc catttttagt ataaatatat cctagaaatc
     4561 ctctatagat ttatttctct tattgctaaa tctatctcac tttgatttaa aggtcttgaa
     4621 tagttgtata tagtacttcc gggtctttca ttatagtatg gtctattaca atccttacaa
     4681 cctcttattt caaaaggata gcctttgttt atgtctttta aaatatcatt gtcaagcttt
     4741 atactattta agtatccatt tttaaactta aaatactctt ttggataacc tttatttatc
     4801 atataagaca ttaactgtac ccttctataa ctttctatgc ttggttgatt tatcttttcc
     4861 attttagttc ctctaactgg agtaaatgca aataaactaa tcgttacatc attttccttt
     4921 aaataaatat ataaattgta tatatcttca tgactttcac ccataccaac tattatatgt
     4981 gtacttattt tattgggata tgattttgac atttcagtta taaattttaa cttttcatca
     5041 tagttatttc cttttatttt ttcatatagt tctttatttg atgcatctat tgctattcct
     5101 attttatcaa ctcctactga aaaaaacttc tttatctgtt catcattttc taattttgca
     5161 gatagtgaaa ttggcatgtc tatcttacca cttatatagt ttataaagtc taaaacagag
     5221 ttatgtgctt cttcacttgc cattgattga atacatattc tctttatatt ttttccttta
     5281 taagctttta aagcatctaa tatttcttct tttgaaaact ctggccaaat tactcttgat
     5341 aatttatctt ttctagtact actttctatt gattgagaac aaaaactgca tttatttata
     5401 catttttctc caatcattat atatgctgtg gtaggaggta tatcgctttt tttgtttaat
     5461 atcccaagct ctattgctgt cccaactgat agtctaatca taaaacgcaa cactccttac
     5521 actcatttat tgtcatattc atttctatag ccttattctt tgcagacctt gatggtaata
     5581 ctattctatt gactccacac attatagata attggtctag ctcttttcta tacactcctc
     5641 ttggtctcat acaacctaga ttaatttcag tatctggtaa attaattcta gcttcacata
     5701 aaatatctgc taccccttct aactcaggag gttctacatt ttcatactca gtcccttttg
     5761 ttggaataag gactataaat gtaagcttat taggaggatt tttctgtagg tattctataa
     5821 tttcatattc accctttatt tgtcctcctt ttaaaccaat acatatatgt ggtgctactt
     5881 ctgtatgttc ttgtattgta ttataaactt caatataatc ttctttactc tttttcatct
     5941 tataaacttc tcttattgtc tcatcatcaa acactaaatc aaaagaaact atatctaaat
     6001 acttacacaa ctctacaatg ctatctttat ccataagacc taaatgagca ttcaatctat
     6061 atcctttctc ttttaagttt ttaatggtat taatatgagt atttataggg acatctccct
     6121 cataactaca accgccactc aatagaaagc tagttatatt ccttgattgt actttttcct
     6181 catattcttc tattggcacc atattgttta agtagtgacc gttgcaatgg gcacatttca
     6241 aactacattg attgcttgta gtactaagag ctagtgtctg gttaggataa gcaaactcta
     6301 ttttatttcc aaaattcttt aatttaatat caaatgctat tttcattttt tcagatagat
     6361 tcttattcat agtttattcc ttcttaagta cattttactc ttctagtaat ttctttgctg
     6421 ttgggaactg ctctatattc gtatgtggta agaaacatgc agaaataaat tcatccatat
     6481 aacttggata tacactaagt tcaacataag ttatttgatt acctatttct tcaagtttat
     6541 tttttgcatc tttacttatg agagctaagt aagaaccaac aagtgaacta ttccctatat
     6601 aagtaaattt ttctctatca atgtcaggaa gtaaccctat aattattgag ttttctatgt
     6661 ctaggttgtt tcctatacca cctgctatat aaaccctatc taatatacta aaatccatac
     6721 ctaaactatc tacaagagtg tatgcacctg aatatatagc tcctttagct ctgatgaaat
     6781 tatctatatc aacttcattg actactatat cattttctaa atcaaattct tctttaaatg
     6841 ctaatacata ctctcctatc tcatgctcat taaatctaac tctcttatta tctaaatccc
     6901 tgtatatttt acctcttcta tctattacac ctttagtaat catttgacag attaaatcaa
     6961 ttataccaga accacatata ccaactggtg cacattcatc aataatcttt aatgttggct
     7021 ctaaagtttc tttatcaata attactttct ctattgctcc tgctgatgcc ctcattccac
     7081 agcttatccc tccaccttca aaagcaggtc ctgctgaaca tgcacagctc atcatataat
     7141 ctttatttcc aaatacaatc tctccattag ttcctaaatc tataaataaa acattttctt
     7201 cacttgacca tatccctgct gataatacac ctgctgttat atctcctcct acataacttg
     7261 caacagatgg agctaagtac acatatgcac tgtcatttac aaaaagacca acattttcac
     7321 ccataagttt tggagatttt aaaaatggtg gtatgtaagg ttcttgtctt aagaagtctg
     7381 tatacacacc caagaataaa cttgtcatag ttgtattccc ggcagccact aatgtaacta
     7441 catcttcctt atctattcca tttctttcat atattgtttt taatagtgga tttatagttt
     7501 cctcaactat agctttatga agtgtctcta atccattttt cttagttgaa tatataattc
     7561 tatttataac atctgctcca tattttatct gtgcatttcc agaagatgct ttatctacta
     7621 cctcttttga atataggtct actaaacaaa caactactga agttgtacca atatctattg
     7681 caactccgta taaggaattt tctttattac cttgttctat atttatgatt gtaagtttct
     7741 tttgtttttg aacatatgtt attgtaacct taaaatcact ctttctaaat acagttggca
     7801 tttttctaag tatatccaat ctaaaatcta tttcattata tcctaggcta tttctaacat
     7861 atctttccaa tctatctaca tcacttatat tatcatctaa atttggttct tccatttcta
     7921 tgtatttctt ttttatattt gttttaaatt gaaggttatg ttcttcaatt atttttttag
     7981 ctctttcaaa aatttcccta tcctcttttt tattactacc ttctatcttc ataccatgca
     8041 tagaagatga tactttagaa ggaacttcaa tttctatatc tgatattacc tttgtacaac
     8101 aagctagtat ataaccttgc tcccactcat catctgttat atgtcttgtt ttttctgtat
     8161 caacttttcc attcaataat tttactttac acttcccaca agacacgttt ccattacaag
     8221 gagcatctat gaatatatct gcatttcttg caacctctag taatatatca ccctcattac
     8281 aataaacctc tttattgttt ggtgtaaaac tcacttttat catgttatcc ctcccaattt
     8341 gttctatata tgataaaagg aaagtatagc taaatttgcg tgcactcaaa ttttactata
     8401 ctttccccaa ttataataat atcttaatct ttaattattc taatccagcc tcgtattctt
     8461 tatcacttaa taacgtatca agttcagcca catcagctag ttttactttt aatatccagt
     8521 tttcatatgg gtcttcattt atacattctg gctcatcttc taatttttca ttaacttcca
     8581 atacctcacc attaacagga gatattaaat cagaagctac tttagaagac tcaactactc
     8641 caaaatctac tcccttagtt acagtatctt ctacttctgg tgtttcaaca aacaatattt
     8701 ctcctaattg atcttgagca tagtctgtta tacctatata tacaacatct ccatcaatta
     8761 cttttaccca ctcatgatct ttagagtatt ttaattctgg taataatttc atttcgatag
     8821 acccccaata ttatattatt gattaaatta cttaaatcat attaatatta tatcatatta
     8881 ttttaaattt tccatcaatt acattacact ttccatagct aatgctggat gacctttttc
     8941 ttctaagaaa gctaatactc cttcagaatc gtcagctata gtttcatcac aaaccatatc
     9001 acagaagttt tctattccag tcatttcttt aactgtagca tttaatttat cagctacata
     9061 atcttttaat tcttttggca tccaaactat tctttctggt ccaccttctg cataagcgaa
     9121 ttttttagaa gatatgaaat gtcttccgtg tcccataaat cctggagttt gaactcctcc
     9181 acctgtcata gaagcaagtt ctccgaaagt cataccaact ggagtaacac ttgcaaattc
     9241 tctgttaact acaacaaatc cgtttgcttc tggcattata ccacagatac attcgaagca
     9301 tccacaagaa gtcattgggt cttctagtat agaatataat gtaacagatt caactgcacc
     9361 ttgagatatt tggtttacag tttcatttac tgaattccat acacctgttc tatcatctaa
     9421 gcactcgcct ttttctattg gttgacaagg acctgttggg tctaattctt tagtagcctt
     9481 agcatctaac cagctaactg ctccacaaag accaagtctt tcaggagtaa caacacaaac
     9541 gtgagctggt gcaaatgatt gacataagtt acaagaatag aaagtatcaa cactttcatc
     9601 aactaatgaa gctaatcttt catctctagc attgtactta gctattgctt ctccttttaa
     9661 ttcagacact ttttcagcat ctgtaattat agttatttca cacttatcaa ctactgattc
     9721 aaattcatct aacatcttag catataaaac ttctcctata tgctcaagtc taaatccttt
     9781 atcaaatgca tctttagaga ttcttaccca agccatatct ctttgtccaa cgtgcattac
     9841 accttctata tagttcaaga agtagtggaa acgtctttca agaactggct cgaaatcttc
     9901 ttgcatattt ttaccagcta ttttaactat tacagcaagc ggtaatctta atacaccatc
     9961 tgcatcaact tcatctatat ttggtcctat tatttctatc ttatggtctt caacttctgt
    10021 tgcttctttc ttaacaacaa gctcccaagc ttcagttctg tttccaccaa actcagcaaa
    10081 catatcgctc tttctaattc tctcaccttc aaatgctgct gcaaatgaaa ctgggatagg
    10141 tatttcagta acttttattt ttattcctct agcttctaat gaagtagcta ctatcttatc
    10201 gtaatctttt tgagttaata ggttcattgg aacttcaaga actgtttggt cagttataac
    10261 tgggaatcct aaagctattg ctccagcacc tgcagataca actaattcac ttaatggtcc
    10321 aaatgcatta acaaatgcag gaactctatt agctgtgtac tctaataatc cgtttaaatc
    10381 accaggagtt aatccaccga atattaatgc tgctcttact gcaacagata caacgtgtat
    10441 aacagaagtt acatcatatc ctagaggtat aactcttagc tcaagaccca tttttactcc
    10501 agcttctata gcttggtcta taactttacc aactaagaaa gttagtaaac ctttagattg
    10561 gtaatctttt ataacttttg ctgctgattc tgcatctgga cactcaccaa gaacaactgc
    10621 aacaccaggt atatctcctg taactaatgg tacaccaagt gatctgatta tagggtcagt
    10681 tatatgacct gcacatggct cactatatgg agcatccata gttgaatatt ttaatgcttc
    10741 aattacttct gcagctaaag ccgtagctaa accagcatta aaagcatgtt ctagtaaatg
    10801 agttctattt attaaagatt ttactacttc taaagcacct tctaagtccc ctaaagtgtt
    10861 catcttttgt cctgttgcag catatataca aggtaatgaa tatgctgtat cagggaaagc
    10921 aactttatgc tcttttccat tcttttctat agcttcagct aacatagctt gagccgcacc
    10981 taaagcttgt tctgaccctg taaagattat attatataga ttcattctat ttcccccttt
    11041 aatatttaca cctatttttt attaactata ccctaaaaac cctagaaagc aaatccacct
    11101 tcatttaact ctaagaaaga gtctgcatat aaagatgccc cgttgattat gtcacaagat
    11161 tttacagttt tcattaattc tgggtcacat ggattcatta tagctgaact aaatccatta
    11221 gccattgcca ttgctaagaa tgcactatct aaaacaggtc taacatgctt aggacatcca
    11281 ttagatacgt tagataatcc tcctgtagtt aaaagtccca tctcagtcat catttttata
    11341 gcttctaaaa cttctacttg tttttcttgc atacctttta taactaagca taatgggtca
    11401 aatagtatat cagttggctc cattcctaac attaagcctt tttctaatat ttcttggcag
    11461 taagccatac gctcatcatt atctcttggg ataccttctt ttgcacataa acctataacc
    11521 attgcttcgt attcagctgc tatatctatt aaatcaaatc ttgagccagc atcagcagag
    11581 tttattattg gttttgcatt agttctatca taaactttaa gtccagcttc gatagctttt
    11641 ttattagctg tatctaatgc tataggaaca ttgttgaact cagattgaag taatttgatt
    11701 ccccatgtca ttatttcttc gccatctctt tcggcaggtc ctatattaaa atctatatag
    11761 tgtgctcctg cttctaattg ctcttttgct ctttttaaga ttggagctgg gtctctttct
    11821 gctaatgctt ttcttattga aggtgatata caatgtattc tctcacctat aatcataaat
    11881 ttttccattt ataatgtccc cctttaaatt taatattaat agttagttaa tattttatta
    11941 gtttgtttgg catgcttttt cttggtatcc ttttaagaac ttaggtaatt ccatagattc
    12001 ctctggtcct ataactacat tccatcctgg taagttatct tctatatcac ctttaagtac
    12061 tgcaactttt cctggaatga taaggtcttt acaattagtt acttcttcta ccttactttc
    12121 tttaatgaaa gcagagattg agtttccacc aaatttacca gcagcccaag atgtaagaac
    12181 tgaatatcca cctgcatctg gtattactaa ccatactgga acttttgatc tttctatatc
    12241 accagcaact atgaagtaag ttaatgcaaa gtcaacagta actaatactg gagaattttc
    12301 atctgggttg ttgattggat aaatctttgg ttcaactctc attggtcttt gtgggtcagt
    12361 gtatatgttt tgtcttagtg caaataatgg taatgcctta gcataactaa tatcatctat
    12421 tactattata gaaccgtatt ttatagtgaa tattgatgat aaagcaactt ccatcatagg
    12481 gtttgaatta gataatctat ttgcaaatac tattgaagga tatccaaatg ttctatcttg
    12541 ctcttttaaa gctgttcttc taacttgtat agcattagtg taagtatctt ttatgttttc
    12601 tcctgttaca tctaatacta attctttata tccagcggct tgaattaatt caactgtttc
    12661 atataattct tctaagctac cagcttttac acctaatggt aaacttgctc ctttaactac
    12721 ttctatcata tctttgtagt tctctttagt tgctccatat accattggtc tagcatcttt
    12781 taatacttca acagcttctt taactacttg tgcatcatca caagctaaaa tataagctac
    12841 ttctaatcca ctgcctttta ttttatctat taattttaaa tacgcatctt tatctccaca
    12901 atactctaaa acagccattt caactttcat ttgttcacca attcttacat agtcaacttt
    12961 tttcatgtta gctattttag catctactgc ttcatcactc atacaagtac agaatgaaac
    13021 tgcatatcta tttctactta ctaatgtttt ttcatgtcta aataatactg tttctccacc
    13081 tagttcatac tcagatgcac cagcaccaac ttttaaagct ttcattaaag gtgcagtagc
    13141 ctctgataat tttgctatag cgtcatcaga catatgtggg catttaccta cttctacagc
    13201 tcctgaagca accttcatag agaaagccat acaagttggg aatccacaat ccttacagtt
    13261 tttctttggt gttaatttaa atatatctaa agcttttaat gccattattt tttcccccta
    13321 accttaacaa tttaattaag ctaatgcgtt aaccaattct tttatagttt ctattgattt
    13381 tggatgacga agtataactg cgtttgaacc acctactaaa acacttgctg cagttgaaac
    13441 ttccattgct atagttcttt cttctggaca accccaatct ggctcatctt ctattggagc
    13501 tatagcttct ttaacatgac ctacttcaaa agctactggt gttatgatag gcatttgtaa
    13561 tgtcttatca ttttgaccaa atgctgcaag tctaattcta tccatagtag atgcaacgta
    13621 ctcatatcca taaccaactg ctgaacatcc aacattcata actatatttt cacctttaac
    13681 gcctagttga gttaacaata cgtttaattg tttagctagg tttatatcaa cagaagattc
    13741 agctcctact ttatgtgcat aagccatacc agctgatgct cctactcctt tgtagttatc
    13801 ttctactgct gacataaata agcagttatg tccatccaat gtttgagcta atttttcaaa
    13861 taatttacca tctttttcat gatttcctga accagctaca actaaaggta attttattgc
    13921 ttcaattact gccttagcta catcagcaca ttcatcaact gacttatcta atccatttgg
    13981 gtcagaacca tcaaatttta aacaaataaa atctgcttgt gtattagctt caacatattt
    14041 agcccactct actggacaat tagctacatc tttgtataat tccttatatg aatcagtcca
    14101 gctttcagga taaacgtctg atatttgtat acctattttt ggagaattac ctacttctcc
    14161 atcaaaacta taaaaaggta atacattttc cccacctaat ttaattgctt tttcccctat
    14221 tcctacttca acttctgata tttttccaga atatttttga gtagacattt taaatgccat
    14281 atttttttcc cccttaagcc tattttaatt ctaatttagc aaaaatttct ttcatagcca
    14341 ctttagattt tgaatcttca ggtaaattaa ctaaaggaat acctgtagaa tcatattcat
    14401 atatcaattc atccataggt acaacaccga ttagctctag gttatgcttt tcaatttctt
    14461 ctttaacacc atcattcata accccatttg gaactttatt tacaatcaaa agtattcttc
    14521 caacacttaa ctttagttcc tcagctaaat ctcttattct agcaacagct tgtatgctac
    14581 gtcttgagca atcactaacc aataaaagtg tatcaacatg tctagtgact tttctactta
    14641 gatgttccat accagcttcg ttgtccatga ctaagtattt gtaatgacca gatattttgt
    14701 ttacttgttc tctaagtatc ccatttacaa aacagtaaca tccttcacct tctgacctac
    14761 ccattactaa taagtcatat ccttcgcctt cttcgattat agaatttaat ctaaattgaa
    14821 gatattgtgc ctttgtcata ccaccaggga atgcattgcc taacttttct ctttgattta
    14881 cttcttctct tatttctcct attgttgctt caacttcgat acctaacact tcatttatgt
    14941 tagcattggc atcagcatct acaactaata ctggtccctt tttgtcctta accaaataat
    15001 caattaaaag ccctgtgaga cttgttttac cagttccacc ttttcctgca acagctatat
    15061 tgtatcccat tttaagactt cccccttaaa tctagccaca gagccttatg tactctgtct
    15121 gtctattata atttaggtac agaatgaaca cattctccat gtacatcatg aagtgcttcc
    15181 attagccctt cagataaact tggatgtgga tgtattgcat cccctacttg ttctactgtt
    15241 aatcctagat gaacagcaag tgacaactct gtcaataaat ctgtagcatg aggtcctact
    15301 actgctgcac ctattatctt atcagtttct ttgtcagcta taacttttac aaatccttgg
    15361 aaatgtccta tagcttgtgc ttttccaaga cctctaaaat caaattgacc tacattgtat
    15421 tctacacctt cagcttcaag ttgcttttca gtcttaccaa caccagcaac ttcaggttca
    15481 gtataaacac atcttggaat tgctctataa tctactacct tagtcttacc taaagcattt
    15541 tcaactgcta cgataccttc tttagaagca acatgtgcta ggaaaggagt atctattata
    15601 tcgcctattg cgtatatacc ttctacatta gtttcaagat gttcattaac tactactttt
    15661 cctctttcca tttcaattcc tatatcttct actccagaat tgtcaaggtt aggtcttcta
    15721 ccaacacata ctaatgcata ttgtgcttct ataacctttc cattagaaag agttgcaacc
    15781 gccttaccat caactacttc acaagtttgt actccaatac ctgtaataac cttaatttta
    15841 tctttcttaa attgtctaag tagttgttta gctacatctt tgtcttcatt taatagtatt
    15901 tggtcaacca tctcaactat agttacttct gttcctaagg ctctaaagaa ctgccctatc
    15961 tcacatccta taactccacc acctactatt aacatagact caggtatctc ttcaagtcca
    16021 agaacttcat cactagttat tacaactttt ccatcatatg ggaacattct tggtacaact
    16081 ggaacagaac catttgctag tattatctta tctgctttta tagtttctac tgttccatca
    16141 tctttagtta cttctatagt gtttttatca attaattttc caaaaccatt aactaagttt
    16201 actcctcttt tttcaaataa gaactctata ccacttatta attggtttac aactttattt
    16261 tttctttcca ttatggcagt gaagtttggc ttaactgtac catctatttc tataccaaaa
    16321 tcctttgctt cttttacagt attcaatact cctgaagaag caagaagtgc tttagttggt
    16381 atacatcctg catttaagca agttcctcca actcttcttt tttcaataac cgttacatct
    16441 gcaccaagca tagaagcttt tatagctgct acatatcctc ctggtccacc acctactact
    16501 actatcttca tccttaattc ccccaaacct aatataatct acaatcttgc aatactataa
    16561 acctgcggca tccagtatag caggtatatt ctcttctgcc ccaattgcca ttatatgaac
    16621 accatcacat aaatcttctt cttttaattg tttgataagt tcccctgcca tttttattcc
    16681 ttcacttacc cagttttctt ttccagctgc tcttaatctt tcgatttgtt catctggaac
    16741 gaatattcct ggtacatttg cagtcataaa tttagccatt cctggtgatt ttaaaggtac
    16801 tattcctgct aataccttgc aatccatatc ccttgttaat tctctaaact ttctcattgt
    16861 atttatgtca taaagtgctt gtgtttggaa aaatttagcc cctgctttta tcttcttttg
    16921 catttttaat aattgaactt ctattggaga atattctgga gttactgatg ctcccagata
    16981 aaaatctgga gaacctttta acttattacc agccatgtcc gtacctgcca ttaaagtctc
    17041 agcagtttga agtatcccca cagaatctaa atcaaacact cccttagcct gtggatggtc
    17101 tcctacgcta gtatggtctc ctgttagtgc caacaaattg tttatcccaa atacacctgc
    17161 tgacaacatt tctccttgta tagctattct atttctatct ctccctgtta tttgaataac
    17221 tggctctaat ccagcatctt ttaataattt acatgtagca agagaagttg ctctcataac
    17281 tgccgattga aagtctgtta catttgctgc atgaactctt ccaactaacg gctttgcaca
    17341 ttctattaaa tgtgaaaggt cagtcccttt tggaggagcc atttcagtag taactgcaaa
    17401 ttttccactt tctaatgttt ctcttaataa gctcatctct aatccccctt ttaaccttct
    17461 aaaaaaaact tttatgaatt tgctgctgct tctttctttt tctttgcact taaacttctt
    17521 ggattatttt gcttagaata gtcttttggt ggtcttattt ctgctaagtt atctaattgt
    17581 cctatagctt ctagtctttc atatatcttt atccaagcac aatcattctc tgagtttact
    17641 tcacacttac catttttagc tccaccacaa gccccattca taagaccttt agcacacata
    17701 gttactggac atatcccacc tgtccaacca agttcacagt ctccacaagc cttacaggct
    17761 tcttcatatt ctccaactct ttgtacttcc cctataaaca aagtattgtt ggctggataa
    17821 actggcttat cttttaaatt cttaactatt gtctgtgtac catctccaca tgctaaagat
    17881 aaaactgcat cagcttcctt agtctcttct tttagagcct ttaaatcctt tttagacttt
    17941 aaaaggttac aagcagggtc aagcattaca tatcctaaga tttttttgcc ttcaccttca
    18001 agagtttcct tcatttttaa aacttcttct tccccaccac ttttacaagt tgctgcacat
    18061 tgattacaac caactaaaac cagcttgtca aaatccttta agtaccctag cacttcttct
    18121 aatggtttat tttcagaaat tatcatatct acttcccccc taaaccttcc acatatatat
    18181 ctctatttat tgttttgtaa tttacaagcc ttaactacat gttttgcaag tatagatgtt
    18241 gtaactgaac caacccccgc tggaactgga gttatcattg atactttgtc taaaactgca
    18301 tttgtatcaa catctccaca taaatttcct tcttcatcaa catttatacc aacgtctata
    18361 actaccgctc catctttaac aaaactttca tcaaccatct tagctctacc aatagctgct
    18421 attagcacgt ctgcctctgc tgctacacct gatagatttt tagtttttga atgacatatt
    18481 gtaacagttg catgttcact taataaaagc attgatactg gctttccaac taccattgat
    18541 cttccaagaa ctgttacctt acttcctttt aaatcaacat tatagtgttt taatatctca
    18601 actactgctg taggtgtaca tggtggaaat ccacttttat ccccttccat taccttagca
    18661 gagtttattg gactaaaaca atctacgtct ttttccggtg caataacata ttttataact
    18721 tcttcattta agtgttttgg aagaggtcta aaacataata taccattaac atttttgtca
    18781 tcattaactc tctttaatac atcaatatat tcctcttgtg ttatatcctc agctaactct
    18841 aacacttctg ttgttatacc tatgttttga catctcttta gtgctcctct ttcataagat
    18901 aaatcatcac ttcttgcccc aactctaact attgttagtt taggatttat accttcttta
    18961 actagtaaat cgacttcttt tattaattct tcactaattt tatcagctac aggttttcct
    19021 tttataattt gtcctttagt tgacattcct tccatacatt cacaactccc ctacaagcaa
    19081 acttaaatta atttattcta tataccacat attaataaca tagtatctac tttcgtcatt
    19141 taaaaattat tttccaagag ctttttcaac tttggcataa acttcatcac aaatttttac
    19201 accatcttca actaacttat ctacttctat ttttacctta ttaacatatt ctttatcttg
    19261 tatagaattt atatttataa ctacatttaa ttgtccacca agtatagcag ctcttaaaca
    19321 ctgaactcct acaccaacat cacttatagc aagtcttgaa cctttatcaa ctaaatcttc
    19381 atgtaactta atagcttcat agcaaactct tactatgttt aatggtactt cacaagcaac
    19441 ttttaatgcc ttttccattg tttctgcttt tattcttttt tcttcttctg tttctttagg
    19501 aagcccgtaa gcttttgata atggtaagaa acattctgca tcatcatcta tcatttttaa
    19561 taaatctttt tctaactttc ctgccttact taatatctct tttacgcttt cttcatactc
    19621 agcatacttc ttcttcccta ttgtaaggtt acataccata ctacctaaag ccatacctat
    19681 tgcacctact agtgcagcag ctcccccacc acctggtact gcttctttgg aagatagtac
    19741 ttctacaaaa tcaacacaag ttttatctgc tattttcatg ataatcctcc ttgaaaggat
    19801 gtaggaaaat atctcaaatt attcgagata ttttacgaac tattttaaga tatctcctac
    19861 tctttattta taaattaatt tccttagaat aatccagcta ttactccatt ttcatcaaca
    19921 tctatttttt ctgctgctgg aacttttggt aatcctggca tcttcattat ttctccagta
    19981 agtgcaacta taaatccagc tcctgctgaa attgttgctt gtcttacagt tattctaaat
    20041 cctgttggtc tacctaattt agtttggtca tcagttaaag aatattgagt cttagccata
    20101 catactggaa ctttagtaaa tccaagtttt tctaagttag ctatttcctt atcagcttca
    20161 ggagtataat ctacaccatc tgctccataa atcttagtag ctatagcatt tatcttgtct
    20221 tttatagata aatcatcttc atagcagaat ctaaagttat tttcttcttc atccaataat
    20281 cttaaaactt ctttagctac tgcaattcca ccttctccac cttttgccca tacttcagaa
    20341 agagcaacat taacacctaa ttccttacac ttgtcttcaa caagtttaag ttcagcttca
    20401 gtatcaagtg ggaatctatt gattgcaaca actgctggta atccatatac ttgagttatg
    20461 ttttcaacat gttttaataa gtttggaagt cctttttcaa gagcttctag gttttcgtta
    20521 tttaagtctg ctttagcaac tccaccattg tattttaatg ctcttacagt agcaactatt
    20581 ataacagcat ctggttttaa gtttgccatt ctacatttta tatctaagaa tttttctgct
    20641 ccaaggtcag caccgaaacc agcctcagtt attacatagt ctgcaaagtg catagccatt
    20701 cttgtagcta taacagagtt acatccatga gctatatttg cgaatggtcc accgtgtaca
    20761 aatgatggtg ttccttcaag agtttgaact aggtttggtt ttaaagcatc ttttaataat
    20821 gcagccatag caccattagc ttttaattgt ctagctgtta caggttcacc ttcatagctg
    20881 tatccaacta ttatgtttcc taatctttct tttaaatcag atatatcact tgccaaacag
    20941 aacgctgcca ttatttcaga agcaacagtt atatcgaatc catcttgtct aactgctcca
    21001 tcaccttttt tacccatacc atcaacgata tttctaagtt gtctatcgtt catgtccaca
    21061 catcttctcc aagttatttt cttagggtct attctaagtg catttccttg gtgaatatgg
    21121 ttatcaagca tagcagctaa aagattgtta gcagctccta tagcgtgaaa atctcctgtg
    21181 aagtgtaagt ttatatcttc cataggaaca acttgtgcat atcctccacc agctgctcca
    21241 cctttaacac caaatactgg tcctagagat ggttctctta aagcaactaa tacatttttg
    21301 tctagtttag caaatgcatc agcaacacct atagtagtag tagtttttcc ttcacctgct
    21361 ggagttggat ttatagctgt agttagtatt aacttagctt ttttaccagt ctctcttttt
    21421 agtagattgt agtcaacttt tgctttgtat ttaccataaa gttctacatc atcttcacct
    21481 agtcctaatt tttttgcaac ctctctaata tcttgaggct tagcttcttg agctatttca
    21541 atgtcagatt taaatcccat aactgaatct cctcccacaa cctaaataga tttaaaatat
    21601 aatttttgta aaaattgtat taatatgtaa aaattatatt ttataagata attatacatg
    21661 taaaattact taaaaactag ctactaagct attaaaatat tcaaacttaa tagctagtta
    21721 tttatctaat tacttactta ttacctatct attgcctatg tctaaccttt aaccaactta
    21781 aaatcccaaa tcaaaatttc tatctatttt atttatcctt aagtgacatt aatttatctt
    21841 ttattgcttt aatctgttct ttcgttgttt cactagaatc atatggagat tgattagctc
    21901 tatcagaatc aataacatct tggttatagt aaataaatcc taaagattct ccatcttcta
    21961 agttttggat tataaattct tcatcttcct tatttctaat tttatttcct actacaaata
    22021 ctttatttac acctatatca tgtccaagtt tcttaacttt tctataagtc tgtagacttc
    22081 tctctcctgg ttctacaact actataaaag catctacacc ttgggctgtt cctcttccta
    22141 agtgttcaat tcccgcttcc atatccataa ccactacatc tttattttgt agtattaaat
    22201 gtgagcagag tctcttcaaa agaacatgct ctggacatac acatccagta ccaccagaat
    22261 ctacggttcc caatgtaagt aatcttactc cattgtattc tttacagaaa ttttcaggga
    22321 tatcgtctac tttaggattc atcttaaaca tttttccaaa tgaacctaca gaagcagctg
    22381 ttctatctga aactaatttt ttcatttctg atataggtac tattgattca tatacttctt
    22441 ttggaaaacc tagtgctaaa gctaaattag catcagggtc ggcatcaaca gcaacaactc
    22501 tgtatccgtc ttcagcaaac atcctagaga gcattgaaga aaaagtcgtt ttacctactc
    22561 cacctttacc tgttattgct atcttcataa tccctaccac cttttattac tatttgcttt
    22621 ttagtagtta attttacagt taaatatttt ttatactcta tatttttata cactaaaaat
    22681 aaactacagc tagtccttac aggaaccaat taaatttacc ctactagctt ttcaaagcag
    22741 catgtatagt tttattattc tttaatattg tttatatttt atttattcag tatattcatt
    22801 atatttcgat gttctttact ttaatatttg actttattca actttactca accagaaact
    22861 tcttataaaa acgtttattc tataacttca ctatttttta agcttcagcc ttttctgcca
    22921 ttttttcttc aactaatgct tcaaagtgaa cacgtttttt ctctatatct tctattattg
    22981 tagctgctaa ctcttttgga tttgtattta cagtaaatgt agctccaact ttatctttta
    23041 actcactagt taatatatcc actgctcttg aagaaccagc tattggaggc attattccta
    23101 aataagtgtc tataccagaa gctacaacat agcatcctat agcaactgct ttttctgaca
    23161 tccattctgg agctatacct acaactggaa ggtcacacat atccatatct aagtaattag
    23221 ctgctgctcc aactaaatct agtatacgac ttatatcaac acaagaaccc atatgtagta
    23281 ctggtgggat tcctactaat ttacagacag ttgcaagacc tttacctgca tatttttcag
    23341 ccgcatcaga ttccattaaa ccaaacttag ctgctgctga agctccacaa cctgtagtaa
    23401 ctactattat gtcgtttttg attaattctt tcattattgt tacgtgagcc tcatttgaaa
    23461 ctcctttagc attgttacat ccaactacac cagctgctcc tcttaatact ccagctttta
    23521 gacagtctgt taatggctta actgttcctg ctgggtctat atgagagttt acaactctgt
    23581 ctaattgatt tactactgct tctacactat acccaactgt tgcgcctgat tttaattcag
    23641 gtataagtac ttttgattta tctctatttt taaagtttaa aatagcttct ttaactattg
    23701 ctttagcatc atcaagtgct tgttcttctc taaattcaat ataagttgaa cctgttatct
    23761 tagcttttgg agaagttgtt acaaatttag tatggtaaca atctgcaact ctagctaatg
    23821 cagggaatat acattgaacg tcaactatta cagcttcaac tgctccagtt attattgcaa
    23881 gttcttgttg atggaagtct cctgctattt ttacaccatg tctcattgta atctcattac
    23941 ctgtacaaca catacctgct aagtttattc catcagcacc aacttcttta gctaaagcta
    24001 ctaagtctgg ctcttctgat gctaaaacta tcatttctga taatgctggc tcatgtccat
    24061 gtaatattat gttaacttta ttttcttcta atactgctaa attagcctct gttcttcttg
    24121 gcactggagt tccaaataag atatcgctta gacgagtacc tatcatagaa ccaccccaac
    24181 catcagccat agaagttcta agactcatgt gtattaaact atctatatca ccagtacatc
    24241 ctatatgagt agagtgcatt attgttgcaa tttctctatc tatagctctt ggctctatag
    24301 cgtattcttt ccatattttt tgtcttggtt ctggtgcatt ttttataaat cttaatgtcc
    24361 caaaaggttt tccaaattcc attaatgcaa tttctgccac ttcatgtgct acatcataga
    24421 tatctctacc ttctgtctct acatcccatt ctttagctaa agtaattaac ttagcttcgt
    24481 ctcttatttt atagtttcca tttggatcag ctaatgctaa tgtatgtgct atatctctag
    24541 catggtctga atgggcagct gtcccaccag caaccattct tgcaaagttt ctacctacta
    24601 tagtatgctc atctgctcca cataaccccc ttggagtctt agggcttatt ctacatggtc
    24661 ccattccaca tattctacag caaacacctt gaagaccaaa accacattgt gccttaaagc
    24721 cttcttttct atcatacatt gtttctacgc cttcttctct agctttagct atcatcttct
    24781 ggctgtttaa atcaattgtt aacatttttt catccatatt cctatcccct ttttttgatt
    24841 tttaaaatcc cccaggtgac tgacttcatg tcataaaata ataaaataaa tgctttctaa
    24901 gttgattata tactaatttt cagtgctttg ctttagattt aaatattttt ttaacaaaat
    24961 tagataatac ataaaaattt tattttctac acttaattta agttgatttt atcgcttgtt
    25021 tttggctatt tgttaatatc taaacctact tttttgatac ttcagtttaa ttaaattatg
    25081 ttttgatttt tttaaatcaa atttgaaatt tttctagtca tttttatatt attgcagatt
    25141 gtttgtgtat ttattatctt tcactactgt ttggacttat tacagatttt tactccctaa
    25201 acttaattag taaaaacttc ttagaatagc attttttgga ctaatttttt tgaatttttt
    25261 tatttttcct actttaatat taattatagc ttcaaattga acatttgtat atattcattt
    25321 gaagctataa ttatttttta tataaaactt ttttaataca gcattaattt agacattttg
    25381 actatttttt ctactttaat tcatttattt caagcgattt atcctatttc atatttttag
    25441 ctacaatagc cccatatgca atagaatata atttagattc atgtgaatca gctcttgaca
    25501 ctaaaactat aggagctttt gcacccataa ttatacctgc tgatttagag tctgccatat
    25561 atgtaaaggc tttccctatt ccattgccaa cctctattgt tggaaccaat atgatatcta
    25621 catctccaga tacttcactt ttaaaccctt ttatctcact agcttctttt gaaacagcta
    25681 aatcaaaagc cagaggacct tctacaatta cattttcacc aaattctcct tctttacaag
    25741 cttttgctaa catatctgca tctacagttg cttgcatctt aggatttact ttctctttag
    25801 ctgccaaaca tgcaactttt acagtttcca ttcctaaagc ttttgcagct tttatagaat
    25861 tttttaatat ctttgccttt tgttcataat caggagctat attcatacct ccatctgtag
    25921 ttataagtaa tttatggtat ttttctaact catataccat aacatgactt aataaactat
    25981 cagttctaag accatagtct ttatttaaca cttcttttaa tatgacagat gtatctaata
    26041 atcctttcat tacaaagtcg gcttctttac ttgatactaa tttaactgct atttctgcac
    26101 actctttgat atcatcctta tccactattt tgataccact taaatcaaat cctatttctt
    26161 ttgatatttc tcttattttg ccttcatttc caaccaatat aggagtaatt atatttcttt
    26221 ctacagcatc ctttattgcc attaaaactt ctttatcatg agctgctgct acagagagta
    26281 caattttctc tcctccctta agttgttcta aaatatcatc taattttttt atcaaaagta
    26341 acccctccct tatatatatt taactaaata ataccatatt tgctatacct tttctcaatt
    26401 tattttttat gttataaaga atttatatga ctaaaagaca gtttaatatt ttattctggg
    26461 atttcatcaa tagctcttaa aagctttgca ggaacaccac ctacaatagt attttttgtt
    26521 acaactgctc cagcagcgat aattgcatta tctccaatag taactcctgg aactcttcat
    26581 acaaaaaaga ggcttatctc tattttgaga tagccccact taaccgataa aattaaatac
    26641 aacctaacaa tatccttact tatttattcc aagttctctt agacaagcaa tagtaatttc
    26701 atcttgtgga tttaattcat aagctctttc tatatagtaa gttgcatcat caatatcacc
    26761 attatttaaa taagccattc ctaaattgca aagtatttct gggtcttcct ttatcttagc
    26821 cgcttgttca aaatactcta ttgctttatc taaattattc ttataagcct cacaaagccc
    26881 caattccact atagtatcca cctgattagg ctttattatc aatatcttct caagatacat
    26941 cttagcctgc tctatttcgc ccataccctt atatccaagt gctatcataa acagcaagtt
    27001 ccaccaatcc atatgttctt cttctaatgg aagtaacttc tctaatcctt ccttaaactt
    27061 accttggaat acaagtgtat atccttcttc atattgaact ttaaagtcca tcttgcctag
    27121 attctcttgt acctcagcta ctaaatcagc atctaaccct agccttaatg cttcttccca
    27181 agtcagctta gtctttaaat attgaccttg attataataa tggtagccta agtgataata
    27241 acctaatgca aaattctcat caacatcaat taccttttca agcttagcca tggcttcaag
    27301 taagaatgca ttcattgcct tatcatccat atctttttga tactgtttag ctatctcttg
    27361 gcaaacaatc gcatagttat acattgcatc taaatcatct ggatacattg ttataaatgc
    27421 ttttatatat attaaagaat ctgtatgttc accaatctca aagtattttc tcgacatata
    27481 tcccatatac gatttcaaat ccaaatcaat atcttttgat aaagcattta aaaacttctc
    27541 atactctgca ttgtttttaa acttactatc tattccaatt atatatatca ttgcatctgc
    27601 tatagacatt gaatttaact tatcttgagc agtcttttct tttatccctt ttactaatac
    27661 ctcatttttt ataggtacat ctaatccgcc ttttggcact ttatagcttt ttaatttaaa
    27721 ctctccacca tgttttatag ttataaaagc taattcttct gcttttttta gcaaatattt
    27781 ttctatctta aaatccattt tatcctccta aatacttaaa tctctacttt atgagtttta
    27841 gatttctcaa atattttttt atcatcatac tcaaataaga ctacattttc tacttctctt
    27901 atttctgtat ttatatcatt ttttaaatcc acaacattaa atctaaatgg ctcaaaatga
    27961 acatatttta ttatatgttt agctggttta tcaacaaaac taggtaaata tgttgataag
    28021 ttatcattat cttgtaaaaa tacatggcat ctattcttaa aatcatccac atctagctta
    28081 ttaaaaaact gtggtatatt tgatgtttta cccagtgata tatagtcata ttttaatata
    28141 tcattgaata catcattatc tatgttgatt ttttcattat agaaatccat caatatttta
    28201 tataactgat tctttccttg tgccaaatca aagtagccat tttcatcaaa atacttggca
    28261 aaaacctcaa agaatttaaa aggactttgt ttataaaatc tgcctataat atatctcata
    28321 gataacacaa agtttttaga attgtagtat ctctccagta tatcttctat atcctttagc
    28381 ttcaaagttt cttcatagct tatataatta ttgtataaaa actcatatgg agcataatcc
    28441 ttatatttaa aaccatgctc atctgcaact tttctcatac ctgtcccttt aatcatcttt
    28501 aaaaagccta attgcagatg ctctatacct agattaaata catcattaaa agaattttca
    28561 aagctcttat aatcttcata aggcagtcct gcaattaagt ctaagtgctg atgtatattt
    28621 ctataagatg ctacagtttg tacaacatga gacaactttt taaaatcatc ccttctacct
    28681 actgcatcaa gtgttttttc attcgttgac tgaaccccta tttcaaattg aaaaagacct
    28741 tctttacaat cagccaaaaa ctcaagcatc ttatcatcta tcaaatacgc tgtaacttca
    28801 aaatgataag ttgtatatcc attatcattt ttcataagga aatccatgat ttccatagcc
    28861 acttttctat ttgcattgaa tgtcctatct ataaacttta tctgtgatac tcttgcatct
    28921 attaaagctt ttaaatcact ctttatcctg tctaaactaa aataccttag acctggaata
    28981 gtagatgata aacagtactg gcagttaaaa ggacaacctc ttgatgattc ataatacact
    29041 attctatttt catattcttt tggattcaag ttttcataag gactaggaat ctcatccaaa
    29101 tcttgaagta attccatagg cttattcact attatctcat tgccttgtct atatacaagt
    29161 ccatttacat catttatttt catctttcct tgcaagtgaa gtactaagtc tctaaaaatt
    29221 aattctccct ctccataaag tatataatcc acaaaatcat actttttcat tgcctcataa
    29281 ctatcataac tcacttccgg tccgcccaaa gctattttag tattaggtct aatttttttt
    29341 agattatcac aaagtttaac tatatctccc acattccaga tatatgttga gaataatatt
    29401 atatcatatt catttttata tatatccttt aaaatatagt ccaaatcgtt gtttatcgtg
    29461 tattctttca tattaacttc tatcaaatct cttacaaatt cttttagata tcttatagcc
    29521 aaatttgtat gaataaactt ggaatttaat gttgttaata aaattttcaa atatttctca
    29581 cctcttttta agattatact gtctttttgt accccttata ggttaaaata gtatatgtaa
    29641 agtctaaata atatctatat aattaattta ttaatttcaa gtaatgtgag gaaaggaagt
    29701 tttttatgga attaattgaa aaagtatcta tcaactcttt
//
LOCUS       DACTGW010000025        29427 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.025, whole genome shotgun sequence.
ACCESSION   DACTGW010000025 DACTGW010000000
VERSION     DACTGW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.025"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1567
                     /locus_tag="I9573_000869"
     CDS             <1..1567
                     /locus_tag="I9573_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAT4795502.1"
                     /translation="ADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVD
                     IRKSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYN
                     LGYTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKI
                     ENRYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDILSESEKVSSIMIENMSQFL
                     NINLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMT
                     EYDNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEW
                     TIVDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKP
                     KKSMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSK
                     LNKGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKI
                     LFKNNLLEKTNGN"
     gene            1557..2006
                     /locus_tag="I9573_000870"
     CDS             1557..2006
                     /locus_tag="I9573_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT4795503.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNISERLLNVHTKKEILDIFYEV"
     gene            2106..2993
                     /locus_tag="I9573_000871"
     CDS             2106..2993
                     /locus_tag="I9573_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT4795504.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGGASK"
     gene            3398..4846
                     /locus_tag="I9573_000872"
     CDS             3398..4846
                     /locus_tag="I9573_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAT4795505.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIIHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            4872..7262
                     /locus_tag="I9573_000873"
     CDS             4872..7262
                     /locus_tag="I9573_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAT4795506.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGIVKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIELKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKDWI
                     NAAQNIYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLNYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKKDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGKNISILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            7264..7935
                     /gene="pgmB"
                     /locus_tag="I9573_000874"
     CDS             7264..7935
                     /gene="pgmB"
                     /locus_tag="I9573_000874"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAT4795507.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLERGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            7974..9374
                     /locus_tag="I9573_000875"
     CDS             7974..9374
                     /locus_tag="I9573_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAT4795508.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNENKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFINKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKESTDNVVEEV"
     gene            9691..10533
                     /locus_tag="I9573_000876"
     CDS             9691..10533
                     /locus_tag="I9573_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAT4795509.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            10678..12606
                     /locus_tag="I9573_000877"
     CDS             10678..12606
                     /locus_tag="I9573_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAT4795510.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKFVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMTIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVASPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            12667..14094
                     /gene="ascB"
                     /locus_tag="I9573_000878"
     CDS             12667..14094
                     /gene="ascB"
                     /locus_tag="I9573_000878"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAT4795511.1"
                     /translation="MSFKDDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYRGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLVGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     INYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            14499..15281
                     /locus_tag="I9573_000879"
     CDS             14499..15281
                     /locus_tag="I9573_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAT4795512.1"
                     /translation="MNMKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQD
                     CLMCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVS
                     SEIVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSK
                     EVEIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNS
                     PKLKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            15297..15662
                     /locus_tag="I9573_000880"
     CDS             15297..15662
                     /locus_tag="I9573_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAT4795513.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(15828..16577)
                     /locus_tag="I9573_000881"
     CDS             complement(15828..16577)
                     /locus_tag="I9573_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAT4795514.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     GKPFIFVCPTYAWRLPIVVTDFIKETKFLGDNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNS
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(17022..17579)
                     /locus_tag="I9573_000882"
     CDS             complement(17022..17579)
                     /locus_tag="I9573_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAT4795515.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITEKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERITFLYSQMERYSEVPPLDSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
     gene            17831..19129
                     /locus_tag="I9573_000883"
     CDS             17831..19129
                     /locus_tag="I9573_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HAT4795516.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEHLNSLAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLIFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVANW
                     IINKYGVTSAFKSIGLVFLIIVCVSSFFIKKCPADFTPEGWVPPEKKGKVRVVINKNW
                     KEMLSTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDSAIVVSILALFNTL
                     GRIVAGYISDKIGRINTLSGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYIGPAVMKNVYSVDGNYQRAFIIAIII
                     ALIGFILTFLYKYINKIQNLKIENLIKGDIKNGKKISKSM"
     gene            19101..21050
                     /locus_tag="I9573_000884"
     CDS             19101..21050
                     /locus_tag="I9573_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT4795517.1"
                     /translation="MERKYPNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMIHGGHGWLINQFLSPYFNKRTDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGTEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVVSVLKPKKVL
                     IAGGGPGGLQAAITAVKRGHKVILCEKTNELGGILKGEQALPFKYKMYELGNTFGKIA
                     KDLGVEVRLNTTVTKEYVENENVDALIIAVGSEPLVPPIEGLDGENVVIVNDYYVEKE
                     KVTDEVIVLGGGLAGCEAAIHLAQEEKTVHLVEMRAELAPDANIRHRPILLEEIERQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDTLLDSAPYVA
                     QIGDCVRASTITNAVYQGHHAALDI"
     gene            21487..21603
                     /locus_tag="I9573_000885"
                     /pseudo
     CDS             21487..21603
                     /locus_tag="I9573_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            21852..22226
                     /locus_tag="I9573_000886"
     CDS             21852..22226
                     /locus_tag="I9573_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAT4795518.1"
                     /translation="MFNLIEMLFPIMFILIFIIIVFTFVKGIATWFKNNNSPRLTVSA
                     RIVAKRQNTTHNNQPNAGDISGAHGFHTISSTSYYVTFQVDSGDRIELSVSGSEYGVL
                     AEGDKGQLTFQGTRYLTFDREN"
     gene            22242..22889
                     /locus_tag="I9573_000887"
     CDS             22242..22889
                     /locus_tag="I9573_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795519.1"
                     /translation="MNKFLSQIRRIDNEISITRKIINTIFILCFGIVLGTFAKFLDTT
                     ASNTLPFIFEYLDISNFFGRFAIWLLIALYIAIYSHSSIRASLNVLVFFIGMVSSYYL
                     YSYFVAGFFPKNYAMIWLGFTVISPLLAFICWYAKGKSKISFILSVIIIAILFNFTFI
                     YGWIYFDVYSILEVIVFGCALIALKRNTFRETTYMILSAVVIAVILNMLVPFHFG"
     gene            23269..24348
                     /locus_tag="I9573_000888"
     CDS             23269..24348
                     /locus_tag="I9573_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAT4795520.1"
                     /translation="MNDMKFIETLKQKRNVCDYSQSRLAQELQINRQNLNEIENGKTK
                     ASKEMKHILLHYLDYCNCTQPFTLTIDYLRVRFPTTDALEIIKNVLAMKSEYFIHEDY
                     GMYAYEEQYIYGDISVNASKDSSMGVLLELRGMGCRDLEYVLQVRGIDWYCFLRCCID
                     YQGVFKRIDLAVNDMGGLLDIEILRERYYANKVWKRSRTHEAVDSGKLSGTHGDTAKT
                     FYIGSKNSSIYFCLYEKEKEQKSKGIKTDIKNRFEIRLKNGKAGQTVEQLVFSRNPEQ
                     TIASFILTQIDFPDYILWDIFLDNVTTSLPFIMTPVAVNMDKIKRWLERQVMPSLLMI
                     KEIEKKTGANYLEEIDRHTRLTEKQ"
     gene            complement(24840..25039)
                     /locus_tag="I9573_000889"
                     /pseudo
     CDS             complement(24840..25039)
                     /locus_tag="I9573_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274515.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25170..25383
                     /locus_tag="I9573_000890"
                     /pseudo
     CDS             25170..25383
                     /locus_tag="I9573_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
     gene            25495..27108
                     /locus_tag="I9573_000891"
     CDS             25495..27108
                     /locus_tag="I9573_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAT4795521.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIVEKIFSLAYQGYGSAKITKVLREEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNTANKTPYSYYACSYYGQFGKGTCSMHYIRYDVLYQAVLKRLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASAFKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEAITNEDNERIQEIEIYYRFIGKVD"
     gene            27137..27460
                     /locus_tag="I9573_000892"
                     /pseudo
     CDS             27137..27460
                     /locus_tag="I9573_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            27492..28679
                     /locus_tag="I9573_000893"
     CDS             27492..28679
                     /locus_tag="I9573_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAT4795522.1"
                     /translation="MSKIVFFSILAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIK
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFITHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            28871..29275
                     /locus_tag="I9573_000894"
     CDS             28871..29275
                     /locus_tag="I9573_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HAT4795523.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYHALYVNKVMLQI
                     YDVENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT        11307 a         3383 c         5346 g         9391 t
ORIGIN      
        1 agcagataaa tattatgtaa gtaaaacttc tatagtaaat gattttaaat atatagaggc
       61 atggataaaa gaatacaatt taaaacttaa taaaacttta gaaggaacta agattatagg
      121 taaagaagtg gatataagga aatcgattgc caaaatgatg gatgatttat tagatgaaaa
      181 ttatgaacaa catgatatat ctgagttaac aagattagat tctgctacat ttagtgcatt
      241 gataaattta tttgatattg attctattat atttgtagaa actataatta cagaattaga
      301 atacaattta ggatatacta taaatcagcc ctactatata aatttaatta cccatatact
      361 tatatgttta aagagaatcg aagaaggtaa tcaaatagaa tctcaggaag aaagagaaat
      421 ttctgtagat aatttggacg agttagtata taaaaatgtc actacactta tagataaaat
      481 agaaaataga tacagagtaa aaataacgaa agatgaaatt atgtacatat atattttttt
      541 ggtatcatct ggatttagta gcgaaaataa tagtagttat aatgatgaat cagatatatt
      601 aagtgaaagt gaaaaagttt catctataat gatagagaat atgtcacaat ttttaaatat
      661 aaatctcaaa aatgatgact tactacaaaa aagtctagca tctcatgtaa ggcctatgtt
      721 aaacagatta agatatgata tccaaataaa aaacccgttg ttaggagaga tacaagagcg
      781 attttcagat gtgctgggat tatgtatgat gactataaat attatgacag agtatgataa
      841 tctgaagaat attagtatag atgaagtttc gtatcttgcc acatattttc aagcggcaat
      901 agaaagaaat atgagtagta aaagagtaat agtagtttgt catacaggat atggaacatc
      961 tcagctcctt gcagccagat taaaaagaga atttcctgaa tggactatag ttgatatagt
     1021 tcctatgcat cagttgagta agcgaaattt agatgatgta gactttgtaa tatctactgt
     1081 aaacttagat ttgaaagata aattacatat agttgtatca gtactattaa tggaaagtga
     1141 tattaataat ataaaaaatg ctttactttc taaacctaaa aaaagtatga acttagatac
     1201 aaacttatta ggaatgtatt tagaagataa tatatatttt aattttgatg tagctcagat
     1261 ggaaagttta ataggtgtga atttagggag taaatatgaa tctatttcat taggaaaaga
     1321 tttgaatata tatatacata agcaaagtaa attaaataaa gggataatga atatcaataa
     1381 tgttactaga acgattgata tttatttaga aattagtaat caaagttttt taaagaatat
     1441 tttaatggaa atatcaaatt tatataggca aaagaactat ataaactata taatagattg
     1501 caagagcaaa gaggatatta aaatattatt taagaataat ctattggaga aaacaaatgg
     1561 aaattagaga aatattaaca gaagaaagca ttattttaga tttgaaatca actacaaaag
     1621 aaggcataat acaggaatta gtagataaat tatacgatac aggaagtata actttaaaaa
     1681 gtaagtttat aaaggcatta aaacatagag aaaaaatagg ctctactagt ataggttatc
     1741 aaatggccat gccacatgga aaaacaaaat atgtaaagaa ggcatccata gtaataggta
     1801 aatctaaaaa aggtgtagat tggccaagta tagatggctt acctgtaaag gtaataatta
     1861 tgtttgctat gccacaatct ggaggcaatg aagatatgat attaatatct aaaatagctt
     1921 acattttagg tggtaaaaat ataagtgaaa gacttttaaa tgtacataca aaaaaagaaa
     1981 tattagatat attttatgaa gtgtaggtat tgtataaatt ataaatttga ggtttttaaa
     2041 ataaaatgtg tattctttta aatagtatag attaattttt agacactaga gaggagagga
     2101 tatctatggc aatatattta gaaatgccta aattaataca tgaatttcct tttcgtagtc
     2161 tatcaaatga aggagaagta ttaacaacac cacactggca taaggaaata gaaatattac
     2221 ttataacaga aggtgtagta aatctattta taaatgacaa gcctatgcaa ttaaaaaaag
     2281 gcgagatagt gataataaaa ggaggagata ttcattatat actgccatca ccgggaagtg
     2341 aaagattagt atttcaattt gatatatctt tttttaatga tttaatttta ttaaatgagg
     2401 aaaaagaaag cttaattaat ttattcagta gtataaaaaa atgtagtaga gattggtcag
     2461 caaaaataag aaagtcagtt ttcgatatat tgatagatat ctacaatgaa gacctataca
     2521 aaatagaagg atatagttat gcaataaaag gtaaaatgta taatttaatt cctatattat
     2581 atagacagat tcctagagaa attgaagaaa atacagtaga atatgaaata aactctaaag
     2641 agatattaga gagattgaac aatatattca aatatgtaga aaaaaattat aaacaaccta
     2701 taaaattaga agatgtgtct tatgaagtag gatttagtgt atattatttt actaaatttt
     2761 tcaaaaaaaa tacaggaaaa acatttataa cttttttaaa tgaatataga ttagaaaaag
     2821 caaagtggat attacttaat aacaatatat ccattgtaga tttggttgaa gaagttggtt
     2881 ttggaagtac aaaaactttt tacagagtat ttaaagaaaa aattggagtc tctccattag
     2941 aatttaaaaa aagatataat agcttggaga aaaaaggggg tgcctcaaaa tgaacttagt
     3001 tcatgaggca ctttctttta tatgaaatca aatatatttt cagtatttca acaatgaaaa
     3061 ttccattcat ttataaagtc taaattttag ttctcaatac aatctctatt tcgaaataaa
     3121 tccttttata tatataaata tacagcaata tatgagaata agataacaat aatgaggata
     3181 taaaaatgta tttgctatat atataattat atacatagat atctaatatt gatatttaat
     3241 atgaaagtat tgaaaataga caatattaat tatagtgagt aaaaaatata attggatttt
     3301 taaattgaat tatttaaaag gtatagtatg aaaaaaatct tataactaaa tatgagatag
     3361 aaattataaa actttatact gtgaaagagg tagatgcatg aaattaggga aagatttttt
     3421 atggggaggg gcaatttcag ccaatcaaag tgaaggattt tatttagatg gaggaaaagg
     3481 tttatctcag tttgataaac tcccattaaa tgataagaga ttaaaagatg tatgtttaga
     3541 tgaggataat attttaaatg aaaataatca atactatcca agtcatatag ggattaaatt
     3601 ttgtgaaaat tacaaagaag atatagcaat gttagcagaa ataggtttta aatgttttag
     3661 attttctata tcttggtcaa gaatattccc aacaggagaa gaaagtgaac caaatgaaaa
     3721 gggattacaa ctttatgata atataataaa agaattaaaa aaatataata tagagccatt
     3781 aataacgata agccactttg atatacctta ttctctagtt gaaaaatatt ctgggtgggc
     3841 tgataggaga gtggttaatt tatatgtaaa atatgctgaa attttgatga aaagatataa
     3901 agatgatgtc aaatactgga ttccttttaa cgaaatgaat atgataattc atattccata
     3961 tataggtgct gggttaactt ttatagctga tgaaaatcaa cttcagaaaa aatatcaagc
     4021 agcacaccat caattattag ctaatgcaaa aactatagaa ataggaaggt atataaataa
     4081 agaatttaag tttgggtgta tgttagctgc tggaaaaaca tatccataca catgtaaacc
     4141 agaagatgta tttgcaactt tagagcatga aaggcataat ttgttttttt ctgatgttca
     4201 agttaggggt gtgtatccaa gctttatgaa ttattattta aaatcaaatc aaataaaatt
     4261 aataataaaa gaggaagatt tatcaacact taaggataat actgtagatt ttgtatcttt
     4321 tagctattat tctagtgctt gtagttcagc tagagctgaa ggtttagaaa aatcaaaggc
     4381 aaacggacca gaaaccataa aaaatccata tcttccaaaa tcaaatagtg tatggcaaaa
     4441 cgaccctgta gggttaagaa taactatgaa tcagttatat gaaaggtatc agttaccttt
     4501 atttatagta gaaaatggac tgggtactca agatattgta aatgaagatg gaaaaataat
     4561 tgatgattat cgtatagatt acatgaaaga acatattaaa aatatgattc ttggagtaaa
     4621 tgaagatgga atagagctta taggatatct tatgtgggga tgtatagatg taaccagtgt
     4681 aagtgaagga aagatgacaa aacgatatgg cataattcat gttgattctg atgactatgg
     4741 aagaggctca tttaaaagaa ctaaaaaaca aagtgctttt tggtataaaa aagtaataga
     4801 aacagatgga gaaattttat ttaatgatat ggaaactcat gcataattca ttttaagaaa
     4861 ggagtgttat tatgagttta atatttacta ctaaggacca acttatagag caagggttta
     4921 gtaaggatac aataaaatcc aatgaaacgt tgtttacact agctaatgga catttaggag
     4981 ttagagggaa tattgaagag tccgatttta ataatgagta tgtagataat atgggtacat
     5041 atgtaaatgg attttatgaa gaatctccaa ttacatatgg agaaagcgct tatggctatg
     5101 caaaacaaaa tcagactatc tgtaagttac ctaatgctca tatagtaaat ttctctattg
     5161 aaggagattg ctttgattta tcaaaaggta ttacaagtga acatactagg atactagatt
     5221 taaatgaagg aattgttaaa cgttcattta tatgggaaaa ctctcgtggt agaaaagtta
     5281 aagtatctat agaaagatta gtatcacaag atatagaaga attaatgatg atttcatata
     5341 caataacacc attgaatttt aatggaaaaa taaaatttaa aaataaaatg gaaaatagta
     5401 tggagaagtt taattgtgaa aaagtagatg acccaagagt ggcatctata aataagaagc
     5461 aatttaaaat taatgtaaaa aaatataaca ataagtattt tatgaattta gttacagaaa
     5521 gaagtagatt agggttatgg tgtgggtttg attcatatat tacttgtgga aatattacaa
     5581 atgagaccta taatgtttca gatgaatgta ttgagcgaga aatagaagtc tatgccaaaa
     5641 aagatgaaag gttaaattta gaaattgttg taggctatag tagagtatat tctgatttaa
     5701 atctaaatga agaacgttta aaatcattag aatgtctgga gtcgttaaaa tgtttagaat
     5761 cagaagcaca tttaaaatca ttagaattgg ttttaagaga agcatgtaat attggatttg
     5821 ataaagtcaa aaatattcag agaaagaaaa tgaatgagtt ttggacaacc tctgatgtag
     5881 agattgaagg cgataaaact cttcaactgg gtataaaaac caatttattt catatttatc
     5941 aatcagcagg aagagatgga aagactaata tatcagcaaa aggtcttaca ggtgatggct
     6001 atgaggggca ttatttctgg gatacagaaa tgtatatatt accatttttt atatatactc
     6061 aaccatccat tgctagagct ttattagatt atagatactc aatcttagat gcatcaagag
     6121 aaagagctag ggaacttgga ataaataaag gttgcttata ttcatggaga actataaatg
     6181 gtaatgaatg cagtgcatat tatccagcag gaacagcaca gtttcatata aatgcagata
     6241 tagcttatgg tattagaacg tattttgaag caactaatga tgaagaattt atgatgcaaa
     6301 atggcttaga aattcttata gaaacagcaa gattttgggt tgaatttgga gattatatag
     6361 aattaaaaaa taataaattc tgtataaatg gagtaacagg accagatgaa tatacagcaa
     6421 tagtaaacaa taattactac actaatttaa tggctaaata taatttaata tatgcagtac
     6481 agatggtaga taaatttaag cataatttat tttcaaatga agtatttaga aaagttaaat
     6541 tacaagaata tgaagtaaaa gactggataa atgccgctca aaatatttat ttaccatatg
     6601 atgaagagaa gaaaataagc aaacaagacg attcattttt agaaaaatca gtatgggatt
     6661 ttaaaaatac accaaaagaa aaatatccat tacttctcaa ttatcatcca ttaacaatat
     6721 atagatatca agtaaataaa caagcagata ctgtactttc agcgttgctt ttccctgatg
     6781 agtttagctt agaacaaaag aagagagatt ttgaatacta tgaaaaaata actactcatg
     6841 attcatcttt gtcaaggtca atatttggaa tgatggcaag tgaaattgga aatcatgaaa
     6901 aagcttataa ctattttatg gacacagcat taatggatat taataaccta caaaaaaata
     6961 caagtgatgg aatacatgct gctaatatgg gtggaacatg gatgagttta gtatatggat
     7021 ttgcaggaat gaaaataaaa aaggataaat taagttttga accaaggata cctaaacact
     7081 ggaaaaatat taaatttaat atattattta gagataattt agtttctgta aatatagaag
     7141 aatataaatc ttattataag ttaatcaaag gtaaaaacat aagcatatta catgataata
     7201 aagaaataaa attatcaaag aacataatag aagttaataa ttgttttaaa gggagtaatt
     7261 aatatgaaag cagtcttatt tgatcttgat ggagttataa cagatactgc taagtatcat
     7321 tttgaagctt ggcatgagat agcaaaatcg ctagatatag aaatagatga aacttttaat
     7381 gaaaaattaa aaggcataag cagggaagaa tcattagata aaatattgtt atctgctaat
     7441 aaagtagata gttatactaa agaggaaaag ttaaaggtgt gtgaagaaaa aaataacatt
     7501 tatataaagt taataaactc attaacatat aaagatatat taccagggat aaatgagctt
     7561 ataaaagaac ttaaaaaaaa taatgtgaaa ttgggaatag catctgctag taagaatgca
     7621 cctaggatat tagaatcttt gggaataaaa gatgaatttg attgtatagt aaatccatcc
     7681 ttattagaaa ggggaaaacc atatcctgat atttttattg aagggagcaa gatgctaaat
     7741 ttaaaacaat atgattgtgt aggaatagaa gattcttatt caggtattaa agctataaat
     7801 gctgccaata tgacatcaat tggtgtgggg aaaaaagaag tactaggaaa atgtgaccat
     7861 attgtagaag atactagctt attaaattac aatacactat tacaagcttg gagtaaaaag
     7921 attaatttgg attaaaatta tattaaaaat tattttaaaa gagaggataa ggtatgaaga
     7981 ataaatatga agaagtagct caattgataa ttgatggact tggaggaaag gataatataa
     8041 ttgacttaac tcattgtatg acacgattaa ggttcatctt aaaagatgaa aagaaagtca
     8101 atgcagataa attaaaatct atagataagg tagttggagt aaactcaact tcaactcaat
     8161 atcaggtcat aataggtaat gaggtaggtg ttgtatttaa tgcaataata aataagggga
     8221 taaataccac aggagatatt aaaagcgata taaaaaatga aaataaaaaa aatagtattt
     8281 taaataatat aatagataca ataactggtt gtatgacacc tatgatacca gcccttacag
     8341 catctgggat gataaaagtt atattatctc tttgtaccac ttttaattgg ctatcatcaa
     8401 attcaagtac atatagagta ttagatataa taggagatgt agcattttac tttatgccta
     8461 taatacttgc aataaatgca gctaaaaaat ttaatgttaa tacatcaata gcagttatag
     8521 tagtaggtgt gtttttacat ccaaatttct ctgcatgggt atcaagtggg gaccctatat
     8581 catttatagg agtaccaatt caaggtgtta tatatgcagc atctgttatc cctgcacttt
     8641 taacagtatg gatgatgtca tatatagaaa aattcattaa taaattaaca cctagcatgt
     8701 taaaaacaat tttaaatcca actttagtat tattaataag tgcgccaata gcattaatag
     8761 taattggacc aataggaaat ttcttaggag agggattagc tagtataata aatttactac
     8821 aaggaaggtt aggatttata atggtttgtt tattagcagc tgcaatgcca tttatcgtta
     8881 tgacagggat gcatcatgca ttgactccta tattcatatc agcatttgca actacaggac
     8941 aagaatcgct aatattaata tctcaagttt gtgctaattt agcgcaaggt ggcgcgacat
     9001 tagctgtagc attaagaagc aaagatagta agatgaaaca gttagcatca gctgcatgga
     9061 tatcagctat catgggtata actgaaccag ctctttatgg agttacattg aaacttaaaa
     9121 aacctgctat gttagcatct ataggagcag gaatcgcagg gttatttgca ggaattactc
     9181 atgtgacttt atatgttcca caaaatagta taatggcagt acttggatta tcaggggaaa
     9241 agggaatttc aaatatagta aatggactta tagttatatt tatagcattg gttgctccat
     9301 ttattatgac atatgttttt ggagttgaaa aaaatgttaa ggaaagtaca gataatgtag
     9361 tagaggaagt ataatattaa ctatttacat taatagaatt tttattatta ttgactataa
     9421 cttgttttgt atttatattg atgcaaagaa ataagttaat attaatgata ataattatta
     9481 taactgacaa aaagcaacac aaaagattga tgaagtatat ggaaataaaa agttgacaaa
     9541 atatgtctga tgttttatca tattaattgg aattaggtag taattgttta agtacaagct
     9601 aagcttattc attaataata gcagtatcct aggaaggagt atgcccatta ttatggataa
     9661 gcttttttta tgcttaaaag gaggataatc atgataatta ataagatatt aaacaataat
     9721 gttgtaatca cattagatga taatgatgaa gaagttatcg ttatgggtaa aggaattggt
     9781 tatcaaaaat caaaaggcaa tctaatagat aaaactaaag taaataaagt atttagaata
     9841 tcaaataaag aaatatcaaa caaactacaa gaattactca ataatattcc tatagaacac
     9901 atgaaattat caagtgaaat tatagaatac gcacaaataa agttaaacaa gaagctaaat
     9961 gaaagtattt acatatcctt aagtgaccat acatattctg caattcaaag aatgaaagaa
    10021 ggaataaatg taaaaaatgc aattctttgg gaaattaaaa gattttataa agaagagttt
    10081 gaaattggaa tgaaagcact agatattatt gaaaacaaaa caggtataaa attaccagaa
    10141 gatgaggctg gatttatagc ctttcatata gtgaatgctc agttaagtga aggtcatact
    10201 ttggcatctg atattacgaa gttaatacaa gaagtactta gcatagttag gtatcatttt
    10261 aggatagaat ttcatgaaga atcagtgttt tattatagat ttataatgca tttaaagttt
    10321 tttgcgcaaa gattgctttt agatagtgca catgaaggtg agacagacaa agaattacta
    10381 agcattatta aatctaaata taataaagag tttgaatgtg tagttaaaat taaaaatttc
    10441 attaaaaaac aatataacta catattaaca gatgatgagc ttatatattt aacaatacat
    10501 ttagctaaag tagttaaaga ctcaaatatg taagtgaaat aatttttcag tagtatatta
    10561 agaatgattg aaaggagtga tactaacaaa attataccta tggttggatg gtaacattga
    10621 gttggccaaa ccttcaaatt tcaaaaaatt aatttaaacg aaatatggag gagaaaaatg
    10681 ggaaaataca aacaattagc acaagagata gtaaacaatg ttggtggaaa agaaaatatt
    10741 agtggattag tacactgtat tacaagatta cgttttactt taaaagatga aagtatagca
    10801 aatgacaatg tattaaaaaa catggaaggt attgttactg taatgaaaag tggaggacaa
    10861 tatcaagtag ttataggaaa tcatgttgaa gctgtctata aagatgtagt tgaaataatt
    10921 ggtttagatg attcaagtac aagttcagaa actaaaaaat caggtaatat actagataaa
    10981 ggtatagata taatatcagg aatttttcag ccagtgttag gaataatggc agcttgtggt
    11041 atgctgaaag gatttaatgc attatttgta gcaatgggat tatatagtga tgtatctggt
    11101 ggttatatgg taataaatgc agcaggagat gcattattta cattccttcc tttgttctta
    11161 ggatatactt cagctaagaa gtttggatta aaaccaatgt tgggattagc actaggagct
    11221 gcagtatgtt atcctgctat acaaggtagc agtatatcag caggagcaga tgtgatgtac
    11281 actttattta aaggaacaat gtttgcatca ccagtatata ttgatttctt tggactacca
    11341 attgtatcaa ttgattatac tggaacagtc gtaccaatta tatttatagt gtattttgct
    11401 tctaggtgtg aaaaattatt taataagttt gttccagatt tagttaagtt tttctttgtg
    11461 ccaatgctta cattattaat aacagtgcct gttgcactta tagttatagg tcctatcgca
    11521 acatttggtt ctacacttat ttcacaaatt gtaatttcaa taagagattt tagtccacta
    11581 ttggcaggtg ctatagtagg gtttacttgg caaattcttg ttatttttgg tatgcactgg
    11641 ggatttattc ctgtatatat aaacaatgtt atgactttag gatatgataa tgttatgatg
    11701 ccattctttg cttgtacatt tgcaagttcg gcagttgtgt tggcaatatt tttcaagact
    11761 aaagataaaa aactaaaaga gatgacaata ccaaacttta tatctggtat atttggagtt
    11821 acagagccag caatttatgg aatattattg cctttaaaga aaccatttat aataagttgt
    11881 attgctagtg ggattggagg cgctttctac ggattcttta atcttagaaa atttataact
    11941 ggaggtatgg gaatatttga acttccagca atgattgaac cagatggggg aatgggcaac
    12001 ttaatagtag ctctttctgg tatagctata tcaatggtcg tagcttttgt attaactatg
    12061 attttatata aagatgagaa agtagaagag tctaaaaaag taagaaataa ggggaaagaa
    12121 gaaataaaag aagtcaagtc tactaaatta gaaagagaaa tagtagcaag tcctataaaa
    12181 ggtgaagtat taaaattaag tgatatagag gatgcagcat ttgcatctgg tgtactaggt
    12241 caaggggtgg caataatacc tagtgatgga aaagtagttg ctccagtaga tggtgtagtt
    12301 acaaccttat ttcctagtct acatgctata gggattcttt ctgatacagg tgttgaagtg
    12361 cttattcata ttggattaaa tactattcaa ttagaaggga gaggttttga ggcatgtata
    12421 aaacaaggtg atagaattac aaaaggtcaa actatattaa actttgatgt tgatgcaata
    12481 aaagagttgg ggtatagtac tgtaactcca attgttatta caaattcatc acaattttta
    12541 gatgtagtag aaacagaaag taaaaatatt gaattagaag ataatctaat tacagtctta
    12601 ttttagtaaa aattgaggca ttagcataag ctagtgcttt ggttataatt taaggaggct
    12661 ttgactatga gttttaagga tgatttttta tggggaggag ctactgcagc caatcaatgt
    12721 gaaggtggat acaatgaaga cgaaagagga ttggcaaatg tagatgtatg tccaacagga
    12781 aaagatagaa cagcagttat tacagggaaa ctaaaaatgt ttgattttga tgatgagcat
    12841 tattatccag ctaaaactgg tattgatatg tatcataatt ataaagaaga tataaagtta
    12901 tttgctgaaa tgggatttaa agtatataga atgagcattg catggagtcg aatatttcca
    12961 aaaggtgatg aagaaacacc aaatgaaaag ggacttcaat tttatgaaaa tatatttaag
    13021 gagtgtaaaa aatataaaat agagccttta gtcactatta cacactttga ttgtccaatg
    13081 catttagtta aaaagtatgg tgcatggcgt aatcgaaaaa tgataggttt ctatgagaaa
    13141 ttatgcaatg ttatctttag aagatataga ggattagtga agtactggct aacctttaat
    13201 gaaataaata tgattttaca tgctccattt atgggtgctg gtatttgctt tgaagaaggt
    13261 gaagatgttg aaaagataaa atatcaagct gctcatcatg agcttgtagc tagtgcaatt
    13321 gcaactaaaa tagcacatga agttgattca aataacttag ttggatgtat gtttgcagct
    13381 ggaagtgtat atccttatag ttgtaatcct aatgatgtat gggaagctac aaagttagat
    13441 agagaaaatt acttctttgt ggatgtacaa tctaaaggta aatatcctaa ctatgcgtta
    13501 aagtatatgg agcaaaaagg tattacacca gaaatggaac ctggtgatat tgaattattg
    13561 aataaatata cagtagattt tatttcattt tcatattaca atagccgttg tgttagaact
    13621 gatgaaaatg cagatgatat ggctgaagga aacatattta catctgctaa aaatccatat
    13681 ctttcataca gtcaatgggg atggccaatt gacccattag gtttgagaat tacactaaat
    13741 catgtatatg atagatatga aaagccacta tttattgtag aaaatggatt aggtgctaaa
    13801 gatatagctg atgaaaatgg ttatgtagaa gatgattaca gaattaatta tctaagagaa
    13861 catataaaag ctatgcatga tgcagtttct atagatgggg tcgatttatt aggttatact
    13921 acatgggcac ctattgattt agtaagtgct ggtacaggtg aaatggaaaa gcgttatgga
    13981 tttatatatg tagatagaga taatagtggt aatggaagtc taagaagaat gaaaaagaaa
    14041 agttttgaat ggtataagaa agtaattgct tcaaatggtg aagatttata ttaaacaaga
    14101 aatctatgtt aaataagaaa tttgtattaa ataagaagag tataaaaatt aataataagt
    14161 ataattttta acttttttaa ctatagaaaa tcaatacaga attattttac attgtattta
    14221 tgcaatagta tgattcacaa ataaaatttt agaaaataaa attgttaaga agtatgggaa
    14281 gtaagtgcaa tttactttgt aataaaagat gaaaaaggcg agcttaggtt ttaagcttgt
    14341 cttttttatt atgtataaac tatgatactt taaaattcta tgaaaaattt atttaataga
    14401 gaaattaaaa atttaatggt aatcaaattc tttaccaatg aatataaaaa atgtctacgt
    14461 gttataatag tttaaactaa taaacgaaag gtagatgaat gaatatgaaa taccaacata
    14521 taatagaagt tgacaaagaa ctttgcattg gatgtgggct ttgtaaaaat gattgtccag
    14581 taaacaatat aattatagaa aataaaaagt cagtaattaa aaaacaagat tgtctaatgt
    14641 gtggacattg tgcagcaata tgtcccacaa aagctattac cttgacagga tttgatgaac
    14701 ctcctataga acttacaaat aagccaaagc tagattcaga tgaattacta atggcaatta
    14761 aatctagaag aagtatacgt aaatttaaag acaaagaagt ttcttcagaa attgtaaagc
    14821 aaattattga ggcaggtagg tataccccta gtgctaaaaa ttcacaagat gtatcatata
    14881 ttgttttaga caataaaaag tcaatttatg aaaacgaagc agtgaaattt tttagaaaaa
    14941 taaaacctat tgcaaatata gctattaaat attcaaaaga agttgagatt gacgataact
    15001 ttttctttaa gcatgctcct attgcaataa tgataattac aaaagataag attagtggtt
    15061 cacttgcagc atcaaatatg gcattaatgg cagagtctta tggtctaggt gttttattta
    15121 gtggatattt ttcagatgta gcaaataatt cgccaaaatt aaaaaagctt ttaggtctaa
    15181 aacgcagtaa tcatgttctt acaacattag taattggata cccagatgtt aaatatagac
    15241 gtactgcaca gaaagaagta gcaactgtta gatacttata agagagggtg gattttatgg
    15301 aaaaagaatg tcaacaaaga atgcaagaaa taacatctaa atttaaagaa tgtcgcaaag
    15361 ctatctctgc tatgggtgat gagactagac aattgatttt aattgctctc ttagagagtg
    15421 attttaatgg tatacgagtg ggagaaatca caaaaaaaac acatctttct cgtcctgctg
    15481 tatctcatca tcttaagata ttaaaagaag cagagattgt taatttgcgt agagaaggaa
    15541 cgaaaaatta ttattatctt gattcaaaaa aatcacaatg gaagtcacta acagagctaa
    15601 taaatcttat atttgttgga atacaacata ttagtgaaaa tgatataaga aaaggtgatt
    15661 aaattatact tgatttaata caaaaagatt gatttaggaa ctaaaaagtt cactttgaga
    15721 cactatcttt tgaactatac ccatcttagg agacagttaa aatatgtaaa actgtataaa
    15781 acaatgggta tagttcattt tataatggtt taaatattaa gaatatatta ttctaaatag
    15841 tatctttctc tgttttgagt tttattttta tactcaatag cttcagtggg gcaaccacaa
    15901 atacatgcca tacaatgagt acagctattt ttccaaactg gctttttgtt ttttaaatta
    15961 atattattta aaggacatag ctctacacat tttccacagc caatgcattt atcagtataa
    16021 tgaaatcctt tggcactaat aattgtctta taaaaaactg tatttattat accactttta
    16081 attgtacctc ctaagccact aggagtaata gtttcaaatt cattttcatt gcgtatatca
    16141 gaaataatcc tatatatttg tttatctgct tctttaatca tttgttttga tgtttctttg
    16201 tctgttgtag aaaataaagc tatataattc tctggcattt taatttctgc catacctttt
    16261 aattgccatt ccttgtattt acatagtttt tgaatatagt ttatggattt tgctgtatct
    16321 cccccacaag tcattacaaa gtaaactctg ttgtctccta gaaattttgt ttcttttata
    16381 aaatctgtaa ccactattgg caatctccat gcataagtag gacatacgaa tatgaatggt
    16441 ttacctgctg atattaattc atctgttttt tcctctttta taagttgatt tagtgatatc
    16501 agttcatcat ttaattcttg tgcaatttta cgagccacat atcggctatt accagtaccg
    16561 ctaaaatata aaatcataat aatatctccc taactatttt ttattttaac tcttaatttt
    16621 ttatttatta tagtacttta ttgaaatatt ctattcaaat gtatgtaaaa tttagtagtt
    16681 aagaaaatta taacacatta gtaattttta tattcattgg taaagaattt gattaccatt
    16741 aaatttagta acaaaatatc aactggattt ttatataaga agagatagta attttcatgt
    16801 gaaagattgt aaatataaag atgattttga agggcaataa gaagataaaa ttgatagctt
    16861 aataaaaaat atttaacttt tatattaact gttttaaata ttataattat ctatcttgaa
    16921 taatcaacca tagaagactg tatacaaaga caaagttgtg tacagtcttt tttataaatt
    16981 taatgtctat aattaaatac actattaaaa tcaataagtt attatttcaa agtagtatta
    17041 tccaaatttt tatgatggtt aatacaattt gttagccatt ttatagcacc ttgttccctg
    17101 ataatagctc catcaagaac tatataatca ccaaaatcat tactatcaag aggaggaact
    17161 tcactataac gctccatttg actgtacaag aacgtaattc tttcttggtg ttgaagcaat
    17221 tgatgctcta gtaaaataag cctagtatct atatctaaat tattagaaaa atacattcta
    17281 agacgaaaaa tatccttagg ggttctttcc attggctcat ccttttcaag ccatttcaag
    17341 aactcatctt tacctttttc tgtgattgtg tataactttt tctctaatac atcaccacta
    17401 attttaatat catatacaat aagcttttca tttaccaatt tttttagttc aggatatatt
    17461 tgactatgct ttgcattcca aaactcagct aattgaaaat tgaactcctt tacgatatca
    17521 taaccagtca taggatttct atttaataat cctaaaattg catactttaa agttcgcata
    17581 aatacacctc tttctttaat tacatactta ttataacacg atatatttag tttgaattaa
    17641 ttaatatagc tatgttttaa taagaacttt taaattatga ggaacaaaaa ataaataaaa
    17701 ctatatataa aaatgaaatt atgtaaatat aaacatgtaa gtatttacat gtttatattt
    17761 acatatagaa aaaattgtga taatcttaac taagaaattc tatttaataa atttatcgag
    17821 gaggagagta atgaatttaa cacaaaaaag atggattata ttaattgcaa gttgtttcat
    17881 caatttatgt attggttcaa tatatgcgtg gagtgtattt gcatcaccta tggcagaaca
    17941 tttaaatagt cttgctggaa caacttttac agctgctagt ttagcaattg tatttacagt
    18001 tacaaactca gttggaccta ttactatgat tacaggggga atgataaatg ataaatttgg
    18061 tcctaaaaaa gttgtatttg taggaggatt aatttttggt ataggtatga tattggcagg
    18121 tttctcaaaa ggattgccta tgttgatttt atcatatggg ataataacag gtcttggggt
    18181 tggcatggtt tatggatgta caataagtaa ttcaattaaa ttttttccag ataaaagtgg
    18241 attagttggt ggtataacaa cagcagctta tgggtttagt tcagtaataa tgcctccagt
    18301 tgctaattgg ataattaata aatatggagt aacttctgca tttaaatcaa taggtttagt
    18361 atttcttata attgtatgtg tttcttcttt ctttatcaaa aaatgtccag cagatttcac
    18421 accagaagga tgggtaccac cagagaaaaa aggaaaagta agagttgtta ttaataaaaa
    18481 ttggaaagaa atgctttcaa cacctatatt ttatctaatg attataatat taacttgtgg
    18541 agctttctat ggattaatgt gtgtgtcatt agcatcttct ctagcaacaa atatgattgg
    18601 gatgacaagt tcagattcag caatagtggt ttcaatttta gccttgttta atacattagg
    18661 ccgtattgtt gcaggatata tatcagataa aattggtaga ataaatactt tatcaggtgc
    18721 attagttatt tcaatttttg gtttattatg tttgttattc tcaagagaag gagatgtagc
    18781 aacattctat attggaataa taattatagg tatatgtttt ggttcattta tgggaatttt
    18841 cccaggattt acagtagata aatttggacc taaaaataat agtgtaaatt atggaatcat
    18901 gtttattggt tttgcttttg caggatatat tggacctgca gtgatgaaaa atgtatattc
    18961 tgtagatggc aattatcaaa gggctttcat tatagctata attatagctt taataggttt
    19021 tatattaaca tttttatata aatatataaa taaaatacaa aatttaaaga tagaaaattt
    19081 aataaaggga gatataaaaa atggaaagaa aatatccaaa tctatgtaag ccaattaaaa
    19141 ttggaaatgt aacattcaaa aacagaatgt tttcagcacc tatgggggga acagacataa
    19201 cagcagattg tacaattggc tcaaaatcaa cagcctttta cgaattaaga gcaaaaggtg
    19261 gagcaggtgc agttacaatt agtgaatgta tggttcatcc agaaacagat ggttcacatg
    19321 catatcatct agacttaaaa atagttgatt ccttagcaag ttttacttat acagcagatg
    19381 caattcgccg tcatggagca ataccaagtg tagaactatc tcattcagga caatattcag
    19441 gaacttattt agttgacaaa gataagaagc gaggattatc acaatgggga gtaagtccta
    19501 gtgttcgtcc tgatggatta gagataaaag aacttacaga agaaataatt gctgaaatag
    19561 tagagtcata tggaaatgtt gcagcattgg ctaagagagc agggtttgag atgattatga
    19621 ttcatggtgg acatggctgg ctaatcaatc aattcttatc accttacttt aataaaagaa
    19681 ctgacaaata tggtggttct ttagaaaaca gagttcgttt tgctaaagaa gttttagata
    19741 gtgttcgtaa agcagtaggt ccaggatttc caatagagtt taggatgagt ggttctgaat
    19801 tatttgaggg agggtatgat ttagattatg gaacagaaat tgctaaatta ttggaatcaa
    19861 gagtagattt acttcatgta tcagcaggta cataccaaag agggtttgca gtaacacatc
    19921 cttctatgtt cttagaacat ggatgtaacg tatatcttgc agaagaaatt aaaaaacatg
    19981 taaatattcc agttgctact ataggagcat taaatgaccc agaaatgatg gaagagataa
    20041 ttgctagtgg aaaagcagat gttatttaca tggctcgtgc attactagct gaccacgaat
    20101 taccaagaaa agttatgagt aatcaagatg aaaaaatagt gaaatgccta agatgcttca
    20161 cttgtatggc agaacgtgct accacttcta caagacgctg cactgttaat ccacttattg
    20221 gacgtgaatt agatggaact gaagttgttt cagtattaaa acctaaaaaa gtattaatag
    20281 caggtggtgg accaggagga ttgcaagcag ccattacagc tgtaaagcgt ggacataaag
    20341 taatactttg tgaaaaaact aatgaattag gtggaatttt aaagggagaa caagcacttc
    20401 catttaagta taaaatgtat gaattaggaa atacttttgg aaaaatagca aaagatttag
    20461 gtgttgaagt acgtttaaat acaacagtta caaaagaata tgttgaaaat gaaaatgtcg
    20521 atgcattaat tatagcagta gggtctgaac ctcttgttcc accaatagag ggtcttgatg
    20581 gagaaaatgt agtaatagtt aatgattatt acgtagaaaa agaaaaagtc actgacgaag
    20641 taatagtttt aggtggagga ttagcaggat gtgaggctgc aattcactta gctcaagaag
    20701 agaagactgt tcacttagta gagatgagag cagaacttgc cccagatgca aatataaggc
    20761 atcgtccaat attattagaa gaaatagaaa gacaaggtat atatgtatat acagaacata
    20821 agggtttaag tgtaacaaca gaaggtgtag tatgtatgaa taaatctggg aataaaataa
    20881 atattccagg aacctcagta atatgtgcac ttggacaaaa accaagaaga gaagtggtag
    20941 acactttact tgattctgct ccatatgttg ctcaaattgg agactgtgtt cgtgcatcta
    21001 caataacaaa tgcagtatac caagggcatc atgctgcgct agatatatag ttaaaaagac
    21061 gtagatagta ttgttttatt ataaaaaagg agtgtctcaa aatgaacttt ttagttcatg
    21121 agacactttt ttacatgaaa ttaaatatat ttttatcatt tcaacaatga aaaaagatta
    21181 tctttatttt gagacagcac ttttgttatt atgattttaa ttcttattgc aagttttcat
    21241 ttgattgatg agtaaaactt tttaagatgt agaagtattt aacaatgtaa atgaccctaa
    21301 cttaaatact ttagcgaagg ttgacagatt tgtgtttgct tttatctatt ataacttaag
    21361 gaaacatgtt acctgaacaa taaatttata taataaaagt atataaatat atatatgaat
    21421 gttaagatta gttgatagaa tttaaataat aaatccaata ttataaacat aggaggtatg
    21481 aaaaatatga ttaatgagaa tttaaaaagt ctaagaaaaa tccaccagta tacacaagaa
    21541 gaattagctg aaaaacttaa tgtttctaga caatcaatag caaaatggga aagtggagtt
    21601 tcccaaacaa aaggatatta ctgaaataac ctgtgtttac ctgcttgtag agaaaagaat
    21661 attgctatta agccagagat actacataaa tgtgttggta tctctttttc gttaatgcaa
    21721 cacagtaaat tatttcattt tcctaattat tttagactga taagtaactt tttaaatgaa
    21781 gaaaagaaat gacaatactt aaaggagcat ggtatactta aaggagaatt tcattaagga
    21841 ggaaaaagag attgtttaat ctaatagaaa tgttatttcc tataatgttt atactgattt
    21901 ttataataat tgttttcact tttgtgaaag gaatagctac ttggtttaaa aacaataatt
    21961 ctcctcgctt aactgtgtca gcaagaatag tagcaaaacg tcagaatacc actcataaca
    22021 atcaaccaaa cgctggtgat atatcaggag cacatggatt tcatacaatt tccagcacct
    22081 cgtattatgt gacatttcaa gtagatagtg gtgatagaat agagctgtct gtatctggct
    22141 cagagtatgg agtgcttgca gagggagata aaggacagct gacattccaa ggcacaagat
    22201 atttaacatt tgatagggaa aattgaattt agaggtgcat tatgaataag tttttgagtc
    22261 agataaggcg tatagataat gaaatatcta ttactaggaa aattataaat acaatattta
    22321 tactgtgttt tggaatagtt ttaggaactt ttgcaaaatt tttagatact acagcaagca
    22381 atactttacc atttatattt gaatatttag atattagtaa tttttttggt cgttttgcta
    22441 tatggttatt gattgctttg tacattgcaa tttatagtca ttcttccata agagcatcat
    22501 taaatgtgtt agtatttttc attggaatgg tttcaagtta ttacctatat tcgtattttg
    22561 ttgctggatt ttttccaaaa aattatgcaa tgatttggct tggttttact gtcatttctc
    22621 cattgttagc ttttatttgt tggtatgcta aggggaaaag caaaatatct tttatacttt
    22681 cagtaataat aattgcaatt ttatttaatt ttacatttat ttatggttgg atttattttg
    22741 atgtgtattc aattttagaa gtgatagtct ttggttgtgc attaatagct ttaaaacgta
    22801 acacctttag ggagacaacg tatatgatat tgagtgcagt tgttattgct gttatactta
    22861 atatgttagt tccatttcat tttggttagg cagattttaa gattattata gatagaatat
    22921 aaatttcacg aaccgccgaa gcgatccgcc agcggtggcg gtcgtaagac atatcgccac
    22981 gccagcttgc tggcgaggtg gcggttcact ttggcggttt ggcgagcctg cgagctttct
    23041 aagcccccgt tatctaacag ggggggacta ttacaaaaga caagagcaaa aacaaatata
    23101 aaactgctga tatgcaacga tttataaggt tgtaagaact tatttcactt tacaccctat
    23161 tctttctacc cgacaggtct aaaacagcat atcaatagaa aataaatata agatacaagg
    23221 tgtatgctcc tatgagtata tgcctttttt tgattggagg aatgaagcat gaatgatatg
    23281 aaatttattg aaacattaaa gcaaaagaga aatgtctgtg attattcgca atcacgactg
    23341 gcacaggaac tacaaatcaa caggcagaac ttaaatgaaa ttgaaaacgg aaaaacaaaa
    23401 gccagtaaag aaatgaaaca tatactctta cattatcttg attattgtaa ttgtacacag
    23461 cctttcactt taacgattga ctatctgcgt gttcgttttc ctaccacaga cgcattggaa
    23521 attataaaaa atgtgctggc aatgaaaagc gaatatttta tacatgaaga ttacggaatg
    23581 tatgcttatg aagaacagta tatctatggg gatattagtg taaatgcttc taaagatagt
    23641 tctatgggtg ttttattgga attaagaggt atggggtgtc gggacttaga atatgtttta
    23701 caggtaagag ggatagactg gtattgcttt ttaagatgtt gtatagatta tcaaggtgtt
    23761 tttaaacgca tagatttagc agtcaatgat atgggcggat tgctggatat agaaattcta
    23821 agggaacgtt attatgctaa caaggtatgg aaacgttcaa gaacccatga agcagtagac
    23881 agcggaaaat tatcgggtac acatggagat actgcaaaaa ctttttatat cggttcaaag
    23941 aatagttcta tctacttttg cttgtatgaa aaagaaaagg aacaaaaaag caagggtata
    24001 aaaacagata ttaagaaccg cttcgaaatt cgtttaaaaa atggaaaagc aggacaaacg
    24061 gtagagcaac ttgttttttc aagaaatccc gaacagacca tagcaagctt tattctcaca
    24121 cagatagatt ttcccgatta tattttatgg gatatatttt tagataatgt aacgacctca
    24181 cttccgttta ttatgacacc tgttgctgtt aatatggata aaataaaacg ctggctagaa
    24241 agacaggtca tgccctcttt attgatgata aaagagattg aaaagaaaac aggagcaaat
    24301 tatctggaag aaatcgacag gcatacaagg ctaacagaaa agcagtaatt aaagattaaa
    24361 caaatgacaa cagacatagc agatatgatt gagaaagata ccgctgttcc acaagggaat
    24421 gacggtattt tttaatttct tcaaaaaaac tgcaacaaaa tgactgcttg tgtacagata
    24481 tggtgcgagg aaaggaaatc taactttttt tgaaaacagg tcattaaatc ggtggctgct
    24541 gtccctatat gatgtgaaag aagaaacgat ttttaatttc aacttaacat ttgacagttt
    24601 tcattcccta tatggagcaa agaaaatctt ttattccata gttggcagtt acggtgttgc
    24661 atgggtagtt agggtgtgaa aagaaaaaca gatttttttc atcaccaact tagcaaactg
    24721 atacttgctt tccctatatg gtgcaggaaa agagagttta actattttca tttcaactta
    24781 gcaactggac gtttgctgta cagatatgta gtgaaaagag aaaaagtttt gaatattggt
    24841 tacgcttcgg ctcttttcca acgcggtata taggaaagac gattttcttc tttcatgttc
    24901 tttgacaact gaataaagca attcaatacg tttgacagac agcgatccat tgaagcagat
    24961 acgccatgac cttttccctg caaaagcgag cgaaccctat ttgtgttcct gtaaaagact
    25021 tgtcctcttt tatcgccatg acctgttctg tctgataaat gatactcccg tacagccaca
    25081 gcttgaacgt tcagagcgtc gcacgcaatg ggtacggcta cataagaact atgcagaggt
    25141 ggaattcctg tgggctatga caaatgccgt tgaattgctt agaaaaaaga ttatggaaag
    25201 ggggtatgtg ttattgctga tattgtaaaa acaatgcaag aaaagacaaa taataagcgt
    25261 agcattggca acagaggaaa aacaaagatt gttgtaaaag agcatttttc cgaaaaggga
    25321 aagacaatgg aggaacttct gactgatgtt atgctggaaa aagcaaagca gacaatcgca
    25381 taaaattggt gcagttgtaa gacatagatt gaatggcaaa aagtacccgt gttatacttt
    25441 acttgcaagc aggtattgta aacacgggtt agttataaag gaggataact gacaatgaaa
    25501 caacagattt acaatactgc actttatctt aggttaagcc gagatgatga attacagggc
    25561 gaaagttcca gcattaccac acaaagaagt atgttgcgtc tatatgcaaa agaacatcat
    25621 ttgaatgtga ttgatgaata tattgatgac ggctggtcgg gaacaaattt tgaaagaccg
    25681 agttttcaaa gaatgattga ggatatagag gcaggaaaaa tcaactgtgt tgtaacgaaa
    25741 gacctttccc gacttggcag gaactatatt atgacaggac aatatacaga attgtatttt
    25801 cccagccata atgtccgtta catagcgatt gacgacggtg tagacagcga gaaaggcgaa
    25861 agtgagattg caccatttaa gaacatcatc aatgaatggg tggcaagaga tacaagccgt
    25921 aaagtgaaat cggcatttaa gacgaaattt gcagagggtg ctcattatgg tgcttatgct
    25981 ccattaggat ataagaaaca ccctgacatc aaaggaaaac tgctggttga tgatgaaaca
    26041 aaatggattg ttgaaaaaat cttttcccta gcctatcaag gttatggtag tgccaaaatc
    26101 acaaaggtac tgcgagagga aaaagttccg acagcgtctt ggctgaattt tacaaggtac
    26161 ggtacttttg cacatatctt tgagggaaag cccgaaagca aacgttatga gtggacgatt
    26221 gctcatgtca aggcaatctt aaaaagtgag gtttatatag gcaacagcgt tcacaatatg
    26281 cagtctacgg tttcattcaa gagcaagaag aaagtgcgta agcccgaaag tgaatggttt
    26341 cgagtggaaa atacccatga accgattatt gacaaagaag tgttctatcg tgtgcaggaa
    26401 cagataaaat caagacgtag acagacaaag gaaaaagcaa caccgatatt tgcagggctt
    26461 gtcaagtgtg cggattgtgg ctggtctatg cggtttgcaa cgaatacggc aaataaaacg
    26521 ccttacagtt attatgcttg cagttactac gggcagtttg gcaaaggtac ttgttctatg
    26581 cactatatcc gttatgatgt gttgtatcaa gccgtattga aacgattgca gtattgggct
    26641 aaggcagtac agcaggacga agaaaaggtg ctgaacaaga tacagaaagc aggcaatgca
    26701 gagcgaatac gggaaaagaa gaaaaaggca agtgccttta agaaagccga gaaccgacaa
    26761 aatgaaattg accgtttgtt tgcgaaaatg tatgaagata gagcctgtga gaagataaca
    26821 gagcgaaatt ttgtgatgtt gtctagcaag taccaaaaag aacaaataga actggaacag
    26881 cagataacca acctaagaga agaattaagt aaaatggaac aggatatgat aggtgctgaa
    26941 aagtggattg agttaatcaa ggagtattcc gtaccaaagg aactgacagc accgttatta
    27001 aatgcaatga tagaaaaaat ccttattcat gaagcaataa cgaatgagga taacgaaaga
    27061 atacaggaaa tagagatata ttaccgattt attggaaaag tagactgata aagagggttc
    27121 atatctttaa ctaagggaaa ctggagaatc tattcctgat ataggtagtt gcattaaact
    27181 tgcaaaattg tacaaggtca aactagatga cttagttaat cattcggaag aaaaaacagg
    27241 tattatagta cctcctaaag gaaagttttt ctttggagca gtagtagttg gtgaacgtgg
    27301 tcagattgtc attccaaaag aagcaagaga agtatttaat ataaatgcag gagataaatt
    27361 attggtactt ggagatgagg aaagaggatt aggaattgtt catcaacgtg atttgataaa
    27421 ttttataggt catatgggag tagtacgtaa agaagaatga ttttaaaaaa ttataaaagg
    27481 aggtatgaaa tgtgagtaaa atagtatttt ttagcattct tgcatatggt catactaatc
    27541 ctactattga agtagttcgt gaattagtgg atagaggaaa tgaggtattg tattattctt
    27601 ttaatgaatt taaagataaa attgaaggtg ctggagcaaa atttatttgt tgtgataaat
    27661 accttccaga attactccct ggagatgaga aaaaaattgg taaggatttt cctggattaa
    27721 ttgagatgat agtagatact actatttctt tagatgaaaa agtttgtagg gaacttaaag
    27781 attttaatcc agattgcatt gtatcagatt cattaagttt ttggggaaag ttatttgcac
    27841 aaaaacttaa tattccatat gtatgctcta caacaacttt tgcatttaat aaacatacag
    27901 caaagttgat gaaacaaaac tttatagaaa ttatacgtat gatttttggt gtaagacgca
    27961 ttaataaaaa aataaagctt ttacaaagaa aagggtatga agtaaaaaat tttatttcta
    28021 tagtttctaa tgataatgat acggatacca ttgtatatac atcaaaagaa tttcaaccaa
    28081 tggtagaaac cttctctagt aagtattcat ttgtaggtcc atcagtatca aagttgatta
    28141 ttaaacctaa agagagaaaa agaaagttaa tatatatttc gcttggtaca attaataata
    28201 aaaatgttag cttttataaa aactgtataa atgcatttaa agattctgat gttgatgtga
    28261 ttatgtctgt tggtagaagt actgatatta aaagtttagg aaatatacca aataattttg
    28321 aggttaaaaa tagtgttgaa cagattgcta tacttcaaaa aactgatgtg tttattactc
    28381 attctgggat gaatagtgtt aatgaaagtt tatattatgg agtacccatg gtattatttc
    28441 ctcaacattc ggagcagaga atggtagcta agagagttgt tcatttggga gcaggaatta
    28501 tgttaaaaga ggataagtcc gaaagtatta aaaaagcagt atttcaagtt ataaatgaca
    28561 atgaatataa agaaaatgca actaaactat caaaaagctt ttataatgca ggaggctcaa
    28621 aaaaagcagc agatgtaata ttacagatta tatgtaatag tagacaagtt gacatataat
    28681 agatttgtta aaaatatata tacatacatg caatttaatc aaagtactga cagtggggtc
    28741 ttttaaccta ccagttgggg catatatatt gatgctaaat atgagggatg ttatatttta
    28801 ataaaaaaga taacatcctt tttttagtgc gttatatcaa gttttaaata ataaatgaag
    28861 gaggattaat atgaaaaaat ataaagttat ttttatagta ttaattatag gtattataag
    28921 tgtaacaggg tttgttgtat ataaaaactg cattaaacca tggaaagatg ctgaaacata
    28981 tattgataag tatatggtaa aacaagatat ttcaaaagat aatataaaaa gtattacaaa
    29041 agaaaaagcc aagaaggcaa gttacgaagg aatactatac aaagtgtatt acaaagatga
    29101 ccctcgatat gagtatcaat atttttatag tgataattat catgctctat atgtaaataa
    29161 agtaatgtta caaatttatg atgtggaaaa taataataaa cttttaagaa gtaatgagga
    29221 attaaaaagt gtaaagtatc caccaatata tcttaaatat aagccagata aatgatttat
    29281 gacaaaatag aatgattttt attaaaattt atttaaaaat atcactagag tgtttaaata
    29341 aaactaaatt taagtattta tatattagta tatcttacta gtgtttaaat aaaattaaat
    29401 ttaagtattt atatattagt atatctt
//
LOCUS       DACTGW010000026        29326 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.026, whole genome shotgun sequence.
ACCESSION   DACTGW010000026 DACTGW010000000
VERSION     DACTGW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.026"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..574
                     /locus_tag="I9573_000895"
     CDS             <1..574
                     /locus_tag="I9573_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HAT4795524.1"
                     /translation="DILSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPK
                     SIIQLIKNISENLNMNPKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINIT
                     KDKKIRETYLLSKEGKSYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLR
                     LKDKVKVENNFDADLEDNRINYINSIGMII"
     gene            575..1018
                     /locus_tag="I9573_000896"
     CDS             575..1018
                     /locus_tag="I9573_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795525.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYINQNKLVDKSKHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            1033..1617
                     /locus_tag="I9573_000897"
     CDS             1033..1617
                     /locus_tag="I9573_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795526.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            1870..2907
                     /locus_tag="I9573_000898"
     CDS             1870..2907
                     /locus_tag="I9573_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase"
                     /protein_id="HAT4795527.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            2988..3353
                     /locus_tag="I9573_000899"
     CDS             2988..3353
                     /locus_tag="I9573_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795528.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINVAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            3358..3675
                     /locus_tag="I9573_000900"
     CDS             3358..3675
                     /locus_tag="I9573_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795529.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            3723..5327
                     /locus_tag="I9573_000901"
     CDS             3723..5327
                     /locus_tag="I9573_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795530.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVVLKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYINVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            5451..7079
                     /locus_tag="I9573_000902"
     CDS             5451..7079
                     /locus_tag="I9573_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAT4795531.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIAKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            7344..8693
                     /locus_tag="I9573_000903"
     CDS             7344..8693
                     /locus_tag="I9573_000903"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAT4795532.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            8849..9928
                     /locus_tag="I9573_000904"
     CDS             8849..9928
                     /locus_tag="I9573_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAT4795533.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            10273..11181
                     /locus_tag="I9573_000905"
     CDS             10273..11181
                     /locus_tag="I9573_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAT4795534.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGVNIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            11186..13555
                     /locus_tag="I9573_000906"
     CDS             11186..13555
                     /locus_tag="I9573_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAT4795535.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRLTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPKDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            13880..14683
                     /gene="proC"
                     /locus_tag="I9573_000907"
     CDS             13880..14683
                     /gene="proC"
                     /locus_tag="I9573_000907"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002846102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAT4795536.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGINTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            15281..18037
                     /locus_tag="I9573_000908"
     CDS             15281..18037
                     /locus_tag="I9573_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAT4795537.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSKFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNTILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEIFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALESKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINGPMLFIDQISTISALEVGNLLMQGKNIEQIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLPNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
     gene            18169..18648
                     /locus_tag="I9573_000909"
     CDS             18169..18648
                     /locus_tag="I9573_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4795538.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTRDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKEIGDLGIDIELRMLPRDKKKQLSDLIK"
     gene            18664..19086
                     /locus_tag="I9573_000910"
     CDS             18664..19086
                     /locus_tag="I9573_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAT4795539.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLANIEYFCMTKDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSIFL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENRNKIKNVM"
     gene            19126..19917
                     /locus_tag="I9573_000911"
     CDS             19126..19917
                     /locus_tag="I9573_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT4795540.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMFDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTTLINAIPKVILDGLGVAGTLLPAVGFALLMDMLISKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAMNTLTEGEIDFE"
     gene            19910..20710
                     /locus_tag="I9573_000912"
     CDS             19910..20710
                     /locus_tag="I9573_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAT4795541.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     KVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFLIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            20758..21813
                     /locus_tag="I9573_000913"
     CDS             20758..21813
                     /locus_tag="I9573_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAT4795542.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRANLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGTISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(21973..23919)
                     /gene="feoB"
                     /locus_tag="I9573_000914"
     CDS             complement(21973..23919)
                     /gene="feoB"
                     /locus_tag="I9573_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAT4795543.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKSNTH"
     gene            complement(23937..24155)
                     /locus_tag="I9573_000915"
     CDS             complement(23937..24155)
                     /locus_tag="I9573_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAT4795544.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            24361..24870
                     /locus_tag="I9573_000916"
     CDS             24361..24870
                     /locus_tag="I9573_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAT4795545.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            24948..25109
                     /locus_tag="I9573_000917"
     CDS             24948..25109
                     /locus_tag="I9573_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HAT4795546.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            25260..25925
                     /locus_tag="I9573_000918"
     CDS             25260..25925
                     /locus_tag="I9573_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAT4795547.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            25991..27775
                     /gene="pepF"
                     /locus_tag="I9573_000919"
     CDS             25991..27775
                     /gene="pepF"
                     /locus_tag="I9573_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAT4795548.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEKYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            28027..28869
                     /locus_tag="I9573_000920"
     CDS             28027..28869
                     /locus_tag="I9573_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT4795549.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVMANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     regulatory      29102..29185
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
BASE COUNT        11761 a         3151 c         5200 g         9214 t
ORIGIN      
        1 agacatatta agcatgattg aatttgaaat aacacaatac attccaataa acataaataa
       61 ctattcaata aaatataaag ttataaattc taaaaatgat aaattgaata tacaagtaat
      121 tcttttccca aagagtatta ttcaattaat taagaacatt tcagaaaatc taaatatgaa
      181 tccaaagact ataaatataa attttgatat attacaaaag cttataaact taaatttaat
      241 agagaatttt tcagaagaag gggtatttat agaatgtaaa aaaagtcagt taattataaa
      301 tataacaaaa gataaaaaaa taagagaaac atacttgcta tcaaaagagg gaaaatcata
      361 tgagttcata aaaagattat taagggattt taagtatgtt tattactatg gtatagaaaa
      421 ttgttttatt gaagaatact ttaaatatac agataaatct attgaagtaa atccattaag
      481 attaaaagat aaagtaaaag tagaaaataa ttttgatgca gatttagaag acaataggat
      541 aaattatata aatagtatag gaatgattat ttaagtgaaa gtggataaaa caataaattt
      601 tcttaatgga tttaaaaaaa ctaaaccatg taataaaatc tataaaataa taatagtctt
      661 aattttaacg tgtattttat ttaaatcatt tatgtatgtg aaatctataa atgaacttga
      721 agattatata aatcaaaata agctagtgga taaatcaaag cacactttac caacaacaga
      781 taaaactaga gagaaatttt ctaaaataaa acttaatgaa ataaaaaaaa tatatgaaac
      841 aattggaata aatagtatta ctaaaatatc gatagataaa aataagctag agatggaagg
      901 tgtgtgtagt gatttagatg ttttaaacaa cctaaggaaa ataaagtaca taaaatatta
      961 ttacatagac agtattgtaa gagatggaga atattataga tttagaataa aaagttagat
     1021 aggtgattat ttttgagatt aaataataga gaaaaaaatc tacttaaaat attgttaata
     1081 ttagttatta tattggcaag tgatatagca gtaagttatt tggaagaaaa cctaactagt
     1141 ttgaagaatg aaaagcaaaa tataataaat aaaataaata atcaagataa ttatcaaata
     1201 aaggataata agcatataga tgataaatat atagaaagtg ttgaattaaa taaaaatgaa
     1261 gaattgaaaa ataattttaa taactcattt ttagataaaa aaacaaataa agatatagtg
     1321 ttattagtag aaaatgaact gagagatatt gtgaatgtag aatatattaa tcaagaaagt
     1381 agtttagata atcacaataa aaaacaagtt aatttagaaa taaaagttaa tggagaactt
     1441 gcaaatgtgt tgaaacttga aggtgtaatg aagaagttaa gactaaataa taaacttgaa
     1501 aagatagaaa ttaacaaatt aaaagtcaat aattttgatg ttcaaaatat tccaaatgaa
     1561 tataaacaag aaaaagacaa tattgttgaa tgtaaaatga cagttagagt catataacaa
     1621 aaaagaatag ataaagtaaa aaactattat caattaataa aattttatag aggaattagt
     1681 tttacgataa ttcatttcta caagagggat tgttgatgat tttatcgaaa tcctaattaa
     1741 agttaatata ttgtaccaaa atataagtga atttaataga atatttactt atactataga
     1801 ttgattaata aaaaaatggt ataataacaa attgtacttt aatttaataa taagggagtg
     1861 gaagtcatat tgttagggag aaatgagcta tgcccttgtg gaagtggcaa aaaatacaag
     1921 aaatgttgtt taaataaaga tattgtttca gaaagagcat gcagaaaaac tgaactttca
     1981 caaaaacaat atacagattt atattcaaag ttatatgatt attcaagaca ggataagttt
     2041 aaagaagaat gtgaaaaagc taaagaaatg ttttatataa tgcaaaatga tagtgtaaat
     2101 gaaaaatttg aaagattttt taatacttat ttaatacaag accacataat ggaaaataaa
     2161 aaggttatga cagttggttt tcttgaagaa aatggtgcaa acctaagtca atcagaagta
     2221 tcaatattaa aaagtatgtt tgaatcatac gtaagtattt atgaggtaaa agaagtttct
     2281 gctggaaaaa tagtattaaa ggactgttta agtggaaatg aactttgtac agaagatgta
     2341 aaacttctga gaagttttaa aatcggaagt tgtatgatag ctagaatagt tgatattgaa
     2401 ggaactaaca tattgataga tataactata agtatatcaa atgaggttaa agatataatt
     2461 ttaaaggata ttatgaatct attcaatcaa tatcaagatg tatacaaaga tatgaagtcg
     2521 ttcttaattt atcatacaca tatactatac aagtatatac aacagctttt agacccaagt
     2581 atagctgaat atttaaagag agaaagagat aaaaacgata caaaagttaa ttcagaaaat
     2641 gaattagctg aagaagaatg taaagtttta gatactctta aagaaaatgt agaaaaagaa
     2701 aatatagatg catgtataga gttttggaat gaatataaga aaaatcatga aaatataaaa
     2761 ggagctgaaa atggttgggc agctgctgtt gaatactatg ttaaaaaaga agcaggacaa
     2821 cctataactc aagttcaaat atcaaagaaa tacgatataa gctctagtac actaggaaag
     2881 agatacaaag atttaaaaat atcttaatta attataaaaa gctttaagtc atagataaag
     2941 aatctatagt ataatatatg tataatgttt agaaagaggt attaataatg agtcttagtt
     3001 taggaaatgc aattgctgta tcaacgaact ttataaaatc taacccagct gtacctgtgt
     3061 acaacggaga agaaatatat gcagttattt atcatataga aataggatat aataatggtg
     3121 tatcacctga taccaaacaa tatataataa tagactataa agagtttgat ttatctatgg
     3181 ataaaaaaaa tgaactaata aatgttgcta aagagtactg taaatctaaa ggtattgtag
     3241 aaaacgaaaa ccttgaaata aaccttttag acaatgaaga agcagagaac ttcttagaaa
     3301 aggtgtttaa taacatgaag gttataagag gtcttataac taaaaaagga taaataaatg
     3361 gactatatag gaatagaaaa tataacacct tatgaaaata catatgaatt tagtgtatat
     3421 gaatatgatg atgaaatcac cttaggtagt gaaaagttat atgtgtgtga attaagggtt
     3481 gtattgatta aagttaattc tctgtatgtt gaaagattgc ataaatcagt tgaagcaatg
     3541 gtcttagtaa aaaacttgaa aaaggattta gataaaacac ttgttgtaaa caaaataaag
     3601 aattttgtgc tagatgagat ttgggtagaa aatctagtaa aagagaatat agaagttata
     3661 tttgtagaaa gctaggaata accctagctt ttttatatta caatctaaga gaggaaaaca
     3721 atatgttaag agtatctaat gtaaaacttg gaattgataa agatatatct ttattaaaag
     3781 atgttgtact taagaagcta aaaataaaag aaaaaaactt agtcaaatat agtatatata
     3841 aagaatctat agatgctaga aaaaaaggaa aaatggaatt tgtttattct gtagatgtag
     3901 aagtcaaaga tgaaaataaa atacttaact caaaaataaa agatgtaaca aaaataaagg
     3961 aaatcagata cataaatgta cctgtgggtg ataaaaaatt aaagaataat cctttggtaa
     4021 ttggtagtgg accagctggt ttatttgcag gtctgctttt agctcaaatg ggttatgaac
     4081 caatcatact tgaaagaggt cttgatgtag acagtagaac caaagatata agtgattttt
     4141 ggacaactag aaaattaaaa aataattcaa acgttcaatt tggagaaggt ggagcaggaa
     4201 ctttttcgga tggtaaactt acaactagaa taaaagatat acgatgtaga aaagtattag
     4261 aagagttagt tcagtttgga gcaccagatg aaatattata ctcacataag ccacatgttg
     4321 gaacagatat attaaaaaat gtagttaaaa atataagaaa tgaaataata agtttaggtg
     4381 gtcaagttag atttaattca aaagtaacag atatagttgt atcaaatgat tctatacaat
     4441 ctgtaataat aaatgatact gaaaaaatag atacagatac aattatacta gctgttggac
     4501 atagtgcaag agatacttat gaaatgttgt attcaagagg tataaaaata gttcaaaaac
     4561 cttttgcaat aggagctaga gttgaacatc cacaagattt aataaataaa gcacagtaca
     4621 aggattttta taatcatcct aggttaggag ctgcagatta tagacttata gagcatgttt
     4681 caaatggaag aacagcatat actttttgta tgtgtccagg aggtagtgtc atagcatctg
     4741 catcaaatga ttttgaaata gttacaaatg gtatgagtga acatgcaaga gataaaataa
     4801 atgcaaatag tgcattttta gtaaatgtaa cgccagaaga ttttaagagt gataatccac
     4861 tagcaggtgt tcatttccag cagaagtatg aaaaattagc ttttgaatta ggtggaaaaa
     4921 attacaatgc accagttcag cttgttggag attttataaa caataagact tctttgaatt
     4981 taggaagtgt tgagccatca tatagaccag gatatacact tacaaattta agtgaatgtt
     5041 tgcctagctt cgtaacagag actatgagag aagcgtttat atctcttgat aaaaaattga
     5101 atgggtttgc aatgtatgat gctgttttga cgggagtaga aactaggtca tcatcaccaa
     5161 taagaatagt aagagatgaa aatactttag aatcagtgag tattaagaat ctatatccat
     5221 gtggtgaagg cgctggatat gcaggtggta tagttacagc agcagttgat ggtataaaat
     5281 gtgcagaaaa aataatacaa aaatacacca ataatacaaa aatttaacat aaacttaatg
     5341 attttttaat atttaacatg aatttaacat tatcactcct ataatttgat aaaatacaat
     5401 aggttataaa atttttagat acagtaatct ctttacaagg aggaaataat ttgaacatta
     5461 taataagtct aatgggtgga ttaggtctgt tcttatatgg tatgaacctt atgggagaag
     5521 gtcttcaaaa atctgctggg acaaagctaa aaaagataat aaaactactt actagtaacc
     5581 tatttatggg agttttggta ggtacaggag ttacagcagt aatacaaagt tcaagtgcta
     5641 ctactgtaat ggttgttggt ttcgtaaatg caggaataat gactttaaaa caggcaatag
     5701 gagttataat gggtgctaat atagggacta ctgttacagc ccaattggta tctttcgact
     5761 taactggtat ggccccagta gcattaggtg taggtattat actttattta tttggaaata
     5821 agcctagaat aaaaaatata gccgaaatac ttataggttt tggtatatta tttacaggta
     5881 tggattttat gaagatggca gtggagccac taagagacta tcaaggattt actgacctac
     5941 ttgttacatt tggtagatat ccactacttg gattattatt gggatttggg attacagcaa
     6001 taatacaaag ttcaagtgca tctatgggta tgttggttgc actggcagca gaaggtctag
     6061 tgccattatc agcagcactg ccaattcttt atggccaaaa tattggtaca tgtgtaactt
     6121 cactgttatc aagtattggt gctaacaaaa atgcacgaag agcagctatg atgcatctaa
     6181 tcttcaatgt tttaggaacg gtgatattct taatattctt gaataaacca gtagtaagca
     6241 tggttacaag ttgggatcca agcaatgttg caagacaaat agccaataca catacattgt
     6301 ttaatataat atctgtattg atacttttac catttacaaa tcttattata aaactagcta
     6361 taaagcttgt tcctgataga gcaggtgata tagatgaaga tgaaactaaa actattaaat
     6421 atatagatga tagaatgata gaaactcctt ctatagctct tgcaaatacg ataaaagaag
     6481 cacttcgtat gggtgagaag gcgaaggaaa gcctaaatgc atctatggaa gccttagtag
     6541 aacattcaac tgaaaaaata gctaagacat atagacgaga gagattaatc aatgatttac
     6601 agaaagctat tttaaattac ttacttaaac tttcaaaagc acctttagat gatgattcaa
     6661 gagaagttgt ggatactttg tttaatacag tcaatgatat agaaagaatt ggtgaccacg
     6721 ctaaaaatat agctgaatta tcgcaagttg caatagattc aaatatttct ttctcagaag
     6781 aaggacaatc tgaacttgat gttatgtata atagagttgt ttcagcatat acttatgcat
     6841 tagaatcaat gagaactgat aatgttgatt tagcttgtaa ggttataaaa attgaagaac
     6901 aagttgatat aatggagaaa tcttgtagag ctaatcacat gtatagattg aacaataatc
     6961 tatgtagtat agaaaatgga gttatttatt tagacgtaat aagtaattta gagagaatat
     7021 ctgaccatgc tgtcaatata gcacaacaag ttatagcgaa gagattagga aatgattaaa
     7081 aataattaat ttaaaataat ataaaattta aaatagagga taaataaaag agaaacttta
     7141 aatgatacgt ttagagtttc tttttttgta aatttatttg actaaaattt ttaaatttat
     7201 acatagttat agtatttaga agaatcgatt tcctgataat aaatgatgag atatgtatga
     7261 ttttgtggca aacttttaaa aaaaacacct atattttgtg ttataatctc tttaaaacga
     7321 atatgctgaa aagagggatt taaatgggaa aaataaattt tgattatagt aaagctacag
     7381 agtttttttg tcaaaacgaa atagatgtca tgcaaccata tgtagatgtt gcgcacgaca
     7441 tgttacataa taaaactggt ttaggaaata cttttttagg atggatagac cttcctaaaa
     7501 attatgataa agaagagttt gacagaataa aaaaatctgc tgagaagata aaatcagact
     7561 cagatgttct tctggtaata ggtataggag gctcttattt aggttcgaga gctgctatag
     7621 atatggtaag tcattctttt aggaatggac ttaaaaaaga gcaaagaaag gctccagaag
     7681 tatattttgt agggcataat ataagctcaa catatattat ggatttatta gatataattg
     7741 aaggaaagga tatttctgta aatgtaatat ctaaatcagg aactactaca gaaccagctc
     7801 tggcttttag aatatttaaa gattacttag agaaaaagta tggtaaggaa gaagcaagaa
     7861 agagaattta tgcaacaaca gatgcaagta aaggtgcact tagacagtta gctacagaag
     7921 aaggatatga gacttttgta ataccagatg atgtaggtgg tcgtttttct gtattgacag
     7981 cagttgggtt acttcctatt gctgctgctg gacttgatat tgatgctatg atgaaaggcg
     8041 caaatgatgc tagagaagca tttcaaaatc ctgacttaaa aagtaatgat agttatagat
     8101 atgctgttgc tagaacaatt ttacatagaa aaggaaaaga tgtagagtta ttagtgaact
     8161 atgaacctca actacattat gtaagtgagt ggtggaaaca gctttatgga gaaagtgaag
     8221 gaaaagaaaa taaaggttta ttccctgctt ctgtagattt ctcaacagat ttacattcta
     8281 tgggtcaata catacaagat ggaaaaaggc ttttatttga gacagtactt aatgttgaaa
     8341 actgtaagag aaatataact ataagttcag aggaagtaga cttagatggt ttaaattatt
     8401 tagcaggtaa gactgtagat tttgtaaatc ataaagcttt tgaaggaact ctattggctc
     8461 atacagatgg aaaagtacct aatttagtaa taaatatacc tcaactagat gagtataatt
     8521 ttggatattt agtttatttc tttgagaagg cttgtggaat aagtggatat ttattgggag
     8581 ttaatccatt tgaccaacca ggagtagaag cttacaagaa aaatatgttt gctttactag
     8641 gcaaaccagg ttatgaaaaa gaaaaggaag agctagaaaa aagacttaaa taattttaaa
     8701 tagttaaggt ttaattaaga ttaaaataat ataatgaaat catcgaaaag gagtttactc
     8761 aaaatagatg atttcatttt ttttatgaaa aattgggaaa aatatatttt gataatttat
     8821 attatattaa gatagggagt ggtatgagat gtcaagaaga aagaaaggga taagtttagt
     8881 aatattagtt gctataataa gttcaatact tagtagtttt ctaacaataa ttttagttaa
     8941 ggacaattta gtaagcaaat ctacaggaag ttctacgccg atagttgtaa atgatgatgg
     9001 taagagtcaa aatatttatc aagcagttgc agaaaaagct acgccatctg ttgtaggtat
     9061 tacaacaact agtgttgata ctagtaatat gtttgcaata ccaactgaaa cacaaggtgt
     9121 aggaactgga atcatagttg attcaaatgg atatatactt actaattctc atgttatatc
     9181 agatggtcaa gctacaagtg taaatgtatt attcaatgat ggttcaacaa cttcaggtaa
     9241 agtagtatgg tttgaccaac aacttgattt agctatagtt aaggttgata aaactggact
     9301 aacaccagca gaatttgctg atagtgacaa agtaaaagtt ggagatatat caatagcaat
     9361 aggtaatcct cttggattag attttcaaaa aacagttact caagggataa taagtggatt
     9421 agatagaact atacaaacag aaaaaactaa tatgactggt ttattacaaa ctgatgcaag
     9481 tataaatgca ggaaatagtg gaggaccatt gttaaatcaa aaaggtcaag taattggtat
     9541 aaacacagca aaagcttctc aagcagaagg attaggattt gcaataccaa tcaatacagc
     9601 taagagtata gttgaagaag taatcaaaaa tggaaaatat gaaaaagtaa ctttaggcat
     9661 aaaaggtaca gatgtatcaa attatgaagc agcaacaggt acaaaattaa gtactgataa
     9721 aggtgtttat gttgcagaag taatatctgg ttcatcagca gaaaaagctg gagtaaaggt
     9781 aggggatatt ataactaaag taggagatac agatataaca ggtatgaatg atttaaataa
     9841 aaagttgtat actttctcaa aaggtgctag tacaaaaata acagtaaata gaggcggaaa
     9901 agcagtaaca ataaatgtga atttctaaat atgaccagaa agaaggagac tacatggtaa
     9961 agtgtagtct ctatttttat ttaattttta tagtatatac atctaaatta attaaaatga
    10021 tgtattgttt atagtttatt gctatatttt cttataaaaa taaatttaat aaatatttaa
    10081 atttaattta caaaaacaat ataaatatca taataaaata aaatttttta atattactaa
    10141 aaatatatag acaaaataat aacaaatata gtattatgta attgtagaaa tgcttttcat
    10201 aggtatattc tatgaagatg aattgcataa aaatgaaaaa atggaatgga tattatttag
    10261 gggtgaaaaa tcatgaatcc attagttata aacttacaaa aatgtagcat acatgatgga
    10321 ccagggataa gaagtacagt attttttaaa gggtgtccac ttgaatgtgt ttggtgtcat
    10381 aatccagaga gtcaaacata tacaaaacaa gttttgtata atgaagaaag atgttcaaaa
    10441 tgtgaagcat gtataaatat atgccctcat aaggcaattt acaaaggtga aacaaaaata
    10501 tgcttagacc aagataagtg tgagttttgt gaaacttgct tagactattg tgtaaacaat
    10561 gcaagagaga ttgttggtca agagtattct gtaagagact tggttaaaga gatagagaag
    10621 gatagaattt tctatgaaga atcgggtggt ggagtaacac tatcaggtgg agaagtaatg
    10681 gctcaagata tggacttcat ttgtggagta ataaacatgt gcaaatcaaa gggaatccat
    10741 gtagcaattg atacatgtgg ctatgcaaag tcagaaaatt atgaaagagt tgctaaatgt
    10801 gcagatttgt ttttatatga tattaagctt attgatgagg ataagcacat aaaatttaca
    10861 ggaaaatcaa atgacttaat actgaaaaat gttaagattt taagtgaatt aggggttaac
    10921 ataaatataa gaataccatt aattgtagga gtaaatgtag atgatgaaaa tttagaggtt
    10981 aaaaaaatga tagagttttt aaagcctttg aatatacaag cagtaagtct tcttccatat
    11041 cataatattg gtaaacacaa gtatgataaa atttataaaa agtatgaagg agaagaacta
    11101 caaagaccat cagaagaaaa attagaggaa ataaaaagac tgtttgaagc aagtaatttc
    11161 aatactaaga ttggagggta ataaaatggc aagaggaact tttgagagaa ctaaaaaatt
    11221 aagagaagaa agtattaatg cagaaccaca catatctatt gaaagagcag tacttatgac
    11281 agaagcttat aaaaaatatg agggaagtgt agagattcct gtacttcgtg ctttatcatt
    11341 taagcattat attgagaata gaactcttag cataaatgat ggagaattaa tagtaggtga
    11401 aaaaggtgat tctcctaatg gagcaccaac atatccagaa atttgttgtc acacaatgga
    11461 agatttagaa gtaatgcaca atagagatat aataaatttt agcgtatcag aagaagctag
    11521 aaaaatacat aaagaagaaa taattccttt ttggaaaaaa agacaaacaa gagataagat
    11581 aataaatgca atgacacctg agtggttagc agcatatgaa gcaggtatgt ttacagagtt
    11641 tatggaacaa agagccccag gacatacagt ttgtggagat acaatatata aaaaaggatt
    11701 tttagattta aagaaagaca tagaagcaag attaaaagag ctagattttc ttaatgattt
    11761 agatgcatat aataaaaaag cagatttaga agctatggca atagcttgtg atgcaatggt
    11821 tatattaggt aaaagatatg ctgagaaagc aagacaaatg gcagaagaag aaacagatga
    11881 agctaagaaa aaagatttat tactaattgc tgaaacttgt gatgttgtac cagctcacaa
    11941 accagaaact tatcatcaag ctatacaaat gtattggttt gttcatatag gggttactac
    12001 tgaacttaat atatgggatg cttttactcc aggaagactt gaccaacatc taaatccttt
    12061 ctatgaaaga gatgtagaaa atggaatact agatagagat agagcacaag aattacttga
    12121 atgtttatgg gttaaattta acaatcagcc agcaccacca aaagttggta taacattaaa
    12181 agaaagtagc acatatacag attttgcaaa tataaacact ggtggaataa atccagatgg
    12241 tcaagatggt gttaatgaag ttagttatat aatacttgat gttatggatg aaatgaaatt
    12301 gatacaacct agttcaaatg ttcaaataag taaaaagact cctcaaaaat tcttaaagag
    12361 agcttgtgaa atatcaagaa aaggatgggg tcaaccagca ttttataata ctgaagcaat
    12421 agttcaagag cttatggaag ctggaaaaac tatagaagat gcaagacttg gtggaaccag
    12481 tggttgtgtt gaaactggat gttttggtaa agaagcatat gttctaactg gatatatgaa
    12541 cattccaaaa atacttgaat taacattaaa taatggatat gacccaatta gtaaaaaaca
    12601 aataggtatt gaaactggag accctagaaa tttccaatct tatgaggaac tatttgaagc
    12661 atttaaaaag caattacatt atatgattga cataaaaata gaaggaaatg ctgtgataga
    12721 aaatatatgt gcaaaacaca tgccttgtcc acttatgtct acaatagttg atgattgtat
    12781 agaaaaaggc aaggattacc aaagaggtgg agcaagatac aacactagat atatacaagg
    12841 tgttggtata ggaactataa cagatagttt aacagcaata aaatataatg tatttgataa
    12901 gaaaaaattc gatatggata cattgcttaa agcacttgat gctaattttg aaggatatga
    12961 agctatactt aatttagttt caaataagac tcctaaatat ggaaatgatg atgattatgc
    13021 tgatgaaata atgcaagaga tattcaatgc atattacaat gaggtaactg gtagactaac
    13081 tgtttgtggt ggagaataca gagtagatat gttaccaaca acttgtcata tatactttgg
    13141 agaaattatg ggagcaagtc caaatggtag actttgtgca aaaccagttt cagagggaat
    13201 ttcacctgaa aaaggtggag atactaatgg acctacagca gttataaaat cttgtgctaa
    13261 gatggaccac ataaaaactg gtggaacatt attaaatcaa agatttgcac catcagtagt
    13321 tcaaggagaa aaagggttag ataatatggc taatctagta agagcatact ttaatatgga
    13381 tggtcatcat atacaattta atgtatttga taaaaatgta ctgttagagg ctcaaaagaa
    13441 tcctaaggat tataaagact taatagttag agttgcagga tatagtgacc atttcaataa
    13501 tttaagtaga acactacaag atgagataat aggaagaact gagcaaacat tctaggtttg
    13561 cttgtaaata aaacttctta ctatataaaa tcgtaactga tataataaag tataaatggt
    13621 ttgattccat ttttaaaatt ttagattaat tatgagctaa ctttctaaat aggaagttag
    13681 ctcgttaagt ttaatgaaaa aatcgttaaa tttatattta gtaaatcata cacatattag
    13741 taagtaatac atacaaaaga ataatttatt atagttataa taaggttata ttttataaaa
    13801 ataagataat tttaagcagt taaaataaag tcaatttagc tactttaaaa taaaactaat
    13861 agaaaaaagg agatttgata tgaaaacttt aggatttatt ggttcaggga atatgggaag
    13921 tgcaatgata ggtggcatag tacagtcagg attagtaaag gcagaagata taactgtatc
    13981 agatttaagc caagttgcct tagataaagt aaaagaagaa tttggaataa atactacaac
    14041 tgattcaaag gtagctgtta caaattctga tgtggtaata gtagcactta aacctaatat
    14101 atgcgaaaag gctttaactc cacttaaaga attaatagat gcaaataaaa taatagtatc
    14161 tatagcagca ggtaaaacta tagaaagtct ggaaagtttt ataggttcag ataaaaaaat
    14221 agtaagagta atgcctaata ctccagccct tgttggcgag ggaatgtcag ctctttgtaa
    14281 aaatagtaat gtaacagatg aagagttaaa tatgattaag gctctgtttg aatcttttgg
    14341 tgaggcagaa atagtatcag aatatttaat ggatacagtt acaggtatta gtggttcttc
    14401 tcctgcatat gtatttatgt ttatagaagc tatggcagat gcagcagtat tagctggaat
    14461 gccaagacaa caagcttata aatttgcatc tcaagcagta atggggtcag ctaagatggt
    14521 tcttgaaact ggaaaacatc caggtgaatt aaaagatatg gtttgttctc cagcaggaac
    14581 tactatagaa gctgttaagg tattagaaga agaaggcttt agagcagcag ttataaaatc
    14641 tattgttgct tgtatagata agtctaaaga tatgagcaaa taacagatgt aattaagttt
    14701 actatatggt ttcttgaatt gtttttttag tacttattta tatctataca taaatgtatt
    14761 tggtataagt aaaataagag taatttacta taaaaaggaa tttaatgatt gagtgagaat
    14821 ttattactta aatattaata attaaataga tatattttta actatatttg agatatatca
    14881 ttgtgaaaaa ataaaattta taagtattat tgtgaggtta taagtagatt tatttaaaaa
    14941 taaatttatt tatagccttt ttaaatttga aataatattc ataaggtaat aaaagttaca
    15001 aatattttct tcgctaataa gttttaaaat acctataatg ttttactttt tattataata
    15061 aatacaataa atatattctt atataaattc tatgtcagat atatggaaag ataagaacct
    15121 tgagattatg ttaaaaaatc tagattttat acttataaaa agtttgattt tacaacctta
    15181 caaaagagaa tcaagatagg aatataatta tctttttcat aaatatataa atgatatact
    15241 aatattatag tattattgat atttgtattg gaggttgaac atgagggatg aaattttaag
    15301 ttttgtaaat gaatgtgtat tatttaacaa ctttgatgaa cttaaaaaag aaagtggcat
    15361 aataacagaa gatatagtaa aaaagtttaa tttaagtaga actcaatcaa gtagattatt
    15421 aaatgattta gtaaaatctg ataatcttat aaagataaac agtagacctg ttattttttt
    15481 acctaaaaat aaaataaatg atgtagtagg acctacaaaa aaaagtattt atgaaaattt
    15541 agatgaactt aaaaatgagt gtaaattatt aaagcaagag actgtgttta atagtatcat
    15601 aggatatgat aatagcttaa aagaagtaat cgaacaaata aaaaccgctg tactttatcc
    15661 aaatggcgga cttacaataa tgctgacagg tgaaagtgga gtaggaaaaa cttttttagc
    15721 caagataata tacaaatata gtgtaagcag taatgtatta gaaaaaaatt ctccatacaa
    15781 tgtgttaaac tgtgcgcagt attataataa tttggagtta ttgtctagtc tattatttgg
    15841 gcatgtaaaa ggagcatata ctggagcaaa tgaatctaga gcaggtctaa tagaacaatc
    15901 agatggtggt gttttatttt tagatgaagt acatagatta aatagtgaag gtcaggaaaa
    15961 gttatttaca tttatggata caggtacgtt ttctaggatt ggagagaatg gagtaattag
    16021 aaaagctagg gtaagattaa tatttgcaac aacagaaagt ctatccaaat ttttgcaaac
    16081 ttttttaaga agaataccca taaatattta tgttcccaat atagaagaaa gagggactat
    16141 tgaaaaaaga aagattgtag aacattttat ttatgaagaa agtaaggtac ttggcttgcc
    16201 tatagagata actcaaatta cattaaatac gattttaaag ataaaattca aggggaatat
    16261 aggtgaatgt aaaaatgtaa taaaatatgc atgtggacga gcttatgcaa aaaatcaaag
    16321 aagaaatgga aatatttttg ttactcttca agacctccct agaaatcttt atatagaaaa
    16381 tgtagatata tttcaattcc aaagtggtaa aagcaaaagc attatctttt ctcctaaaca
    16441 atttagtatt gaaatgtcaa atggaagaaa taaaagaaat ataatctatc aaagtttcat
    16501 aaaatgttta gactactttg atagactaga aaaaaatgaa ataacaaaag aaatatttag
    16561 taaaaagagt tctaatctaa tgattagact aatggacaat ttagtctttt caaataatga
    16621 tgaaaataat aacgtactta tggagcttat aatatcgata atgcaggata tatttagaaa
    16681 tttagaagtt aataacaata taaaatacga tgggaataat gtattggcat tttcgtctta
    16741 tctttatttg aaggacgaat cttatcaatt atctgatgag tataaagctt tagaaagtaa
    16801 attactaata tatttaaaaa aagagagtta caaagaatac tctataataa gtaaaatatc
    16861 aacattaata acgaatagat tggatatgta tcttaataat gtagatacaa tcataatgat
    16921 tatatttatt aaatcaacaa ttataagaat gaaatttaat caagttaatg cgactattat
    16981 agcacatgga tatgctactg ccagtagtat ggcgaatgta tgtaatcgga tgttgggtaa
    17041 aaatatattt acagctattg atatgcctat agatgctacc attgctgatg tatcaaaaca
    17101 aatgtctaga tacctagaag gagataggat aaagaatggt ttaattgtgt tatttgatat
    17161 gggctctctt aatcttatct atgaagattt aaaaaataag ataaatgggc caatgttatt
    17221 tatagaccaa atatccacta tttctgcact agaagtagga aatttattaa tgcaaggtaa
    17281 aaacattgaa cagattgcag aaattatgga tgaagaaact aaacctaata tacaattgta
    17341 ttatcctcaa aaaaataaag aatatgctat aatatcatct tgttttacag ggataggtac
    17401 tgctataaaa atccaacatc tactttccga aagtttaatt gggatagttg atgtcaaaat
    17461 aataccttat gattatgata gtcttgtttc tatgggagat aaagaaccag tttttgaaat
    17521 gtatgatgtg atggctatag taggaactgc taatccaaat gtgaatgggg tggattttat
    17581 attgttagag gatataatat caggaaaagg agaagatgat gtttttagaa tatttagtag
    17641 agtagtagaa aatgaaaaaa taaagaacat aaatgattct attgtaaaga atttttcatt
    17701 aattagagta atagacagtc taactatttt agattcaaaa aagataatag agagaataga
    17761 agaaggaatc aatgctatag aaaatattca aaagaaaaaa cttccaaatg ataaaaagat
    17821 ttctttatat gttcatttaa gttgtatgat tgaaagatta gttagacaaa ctgaaattga
    17881 agagtatact gatatggatt tgttaatcaa aaaccatcat agtgaagtaa aactaataaa
    17941 aaatgcattt agtgtgctag aaaagagtta tagtgtgcaa ataccaattt cagagatagg
    18001 atatatttat aatattatct atggtctacc acagtaaaat acaagattac aaaataagta
    18061 aatgaaattt aaaatagtat gccagtattt ggcatacttt ttgcttttat agataaatga
    18121 gagtataaac aataaaaatt ttaatgtatg atggaggatt tggggaaaat ggtatctttg
    18181 atgagaattg atgacagatt gatacatggt caggttgcat atggatgggc aacatcacta
    18241 ggtataaatg taatactagt tgcaaatgat gaagcaaaaa atgaccaaat gaaagctatg
    18301 tcactgaatt tagcgaaacc tagcaatgta actttatata taaggggggt agaggaatct
    18361 ggagaaatag tagaaaaatt tgcttcttca aagaaaagca atgtattagt gttagtaaaa
    18421 aataccagag atgctttagt tgtagctaaa aattcaggag gagtattaaa agaaataaat
    18481 gtaggagggt taagatatga agaaggaaaa aagaaactta cagatttagt tgctgttgat
    18541 gatgatgata taaataactt taaagaaata ggtgatttag gaattgatat agaacttaga
    18601 atgttaccta gagacaaaaa gaaacaacta tcagatttaa taaaataatt taggagaaag
    18661 agaatgaaaa aatttctaat tgcaacacat ggagaattgt ctagagaact tgttgacaca
    18721 agtaaattaa taattggttc attagctaat attgagtatt tttgtatgac aaaagataaa
    18781 tcagcagatg atgctgagaa agaaattaga agtatattat caaataataa agatagtgaa
    18841 gaatttgttg ttttaacaga tgtgtttggt ggaagtgtgg caaatatatg tacaaactta
    18901 cttttgcaag ggttcaaatt tgaattgttg acaggcgtaa atttaccaat gcttttatct
    18961 atattcttat tagttgagga ggatacaaaa ctaatagtta aatcaggctt agaggaagca
    19021 aaaaatggaa ttgtttatgt aaatgagtta ttagaaaatc gaaataaaat caaaaatgta
    19081 atgtaattta atgtaaatta aactaagtta aaatggaggt tcaatatgtt agtacaagct
    19141 actatgttag ctattatagc aggagttggt atactagatg ggcgtatatt tggtcaatct
    19201 atgtttgata ggccaatagt tacaggtatg ttagttggat tagttttagg agacataaaa
    19261 tcaggaatta tgattggtgc acagcttgaa ttaatttgga tgggaattgc agggataggt
    19321 gcagctactc ctccagacgt agtaacagga ggtgtattgg gtacagcttt tgctatatta
    19381 tcaggtaatg gtgcagaagt tgctttagca gtggcagtgc cagtagcagt attagcacaa
    19441 tctcttggag tattagttag aataataaat tcgtactttt ctcaaaaggc agttttctat
    19501 gctaaacagg ctgattttaa gaaagttacc ataatgatgt ggttgccagt aatattattc
    19561 tttttaagta catcagttcc aaccttttta gcaattatga ttggtgctga taaagttaca
    19621 acattgataa atgcaatacc taaagttata ttagacggat tgggtgttgc aggaacattg
    19681 ttgccagcag taggttttgc gttgcttatg gatatgttga tttcaaaaaa gatgtctgta
    19741 tttttcttta tagggttttt gttagcatct tatggtggat tagatataac tgctatagct
    19801 ttatttggag tatgtgtagc atttgtacta aacttttata tcaataacaa agaaaataat
    19861 caaaatcctc aattagcaat gaatacttta acggaaggag agattgactt tgaataaatt
    19921 aacaaaaaaa gaattaagaa gtatgttttg gcgttcattt gctcttcaag gtgcatttaa
    19981 ttacgaaaga atgcaaaact taggttattg ttattcaatg ttacctgcaa taaagaagct
    20041 atataataag aaagaaaatc aagcaaaagc aatagaaagg catttagaga tttttaatac
    20101 aacgacagtg gtagttcctg ctatattagg tataacagca gctatggaag aagaaaatgc
    20161 taataatcca gaatttgatg aaagttcaat tagtgctgtt aaaacagctc ttatgggacc
    20221 tcttgcagga attggtgatt cattattctg gggaacattt aggattatag cagcaggagt
    20281 aggagtatca cttgcaaaag aaggcaatat ctttggacca ctattattct tattattata
    20341 caatatacca gcatttgccc ttagaatatt tggacttaag tatggttata aagtaggggt
    20401 aaattctctt gaaagaatac aaagagaggg acttatggag aagataatgt ctatggctac
    20461 aactgtcggt ttattcgttg taggaggaat ggttgcaact atgttgagta taactactcc
    20521 tttgaaattt aacttaaatg gtgcagaagt aattttacaa gatatactag ataaaataat
    20581 acctaatatg ttacctttaa cctttgcatt tttaatatat tacatgttga agagaaaggt
    20641 aagtgtaaca aaattaacta taggcactat agtaacagga atcgctttac atgctatagg
    20701 tttgttatag aaattaatta taatttattg aatatataga taaggaaggt gtataaaatg
    20761 acaatacaag attatatgtt agaaacacca gttagaatga gagaaattat atctaatgca
    20821 gatagcttat ttaatgaagt taagagagca aatctaaaaa agattataat aacaggttca
    20881 ggtactagct atcactcagg agtgcaagtg caaccatatt tacaaaattt attagatata
    20941 gatgttgtga aaatgtatcc atttatgata actgaagata cattcaagtt tgataatgaa
    21001 aataccctag tagtaggtgt atctcaaggt gggagtagtt attctacata caacgctatg
    21061 aagttagctg aagataaggg ttgtaaaata gcatctatgg caggttgtaa gaatgcttta
    21121 atagatgaga tttctgatta tatattaaca gtaaattgtg gtgaagaaaa atctggagct
    21181 aaaacaaaag gatattattg tacaaagtta aacttaatgt tattaggtct tcaaatagct
    21241 agagaaaaag gtacaatatc tagtgaaaag tacaatgagg aaataaataa gatattagat
    21301 gcaattaata gatttgaagc agtgtataag ctctctaaac aatggattga aagaaacaaa
    21361 gaaaagttag ttaattctaa agaaataaga ataataggtc actctgatat atatggagat
    21421 acattagaag cagcattaaa attattggaa acaatgagaa tacctgtaac tgggtatgag
    21481 tttgaagagt ttatacacgg tatatataat gctataaata gtgattctac tatatttata
    21541 ctagatacag gaaaagaacc tagagtaact aagatgattg atgtactttc tggttggacg
    21601 gaaaatgtat ttgctatagg tagagatgtt acagaaaatg ataaaaattt aaagattgat
    21661 atcacagata atccttacta tcaaacattt aattttatag tacctataca actaatatgt
    21721 ggggaaatac ctacattaag aggcgtagac ccaagtgttc caaaagatac taggttccac
    21781 atgaagttag gtagcaagaa actgaataag taacaaaaaa agaggttgtc tcaaaataga
    21841 gataagcctc tttttgactg ttgaaatgat gaaaatatat ttgattttat ataaaaaaga
    21901 gtgcctcatg aactaaaaag ttaattttga gacacccact tttatcttat tttaaactaa
    21961 aatatttata tattagtgtg tattactttt tttattgctg tcttccttac ttgagcaaga
    22021 attagcactt ggacatccag agcaaataga acatccacta cagacatttc cgttttttgt
    22081 agacttgaat ttattaaaca gaataaagag tgcaaggact ataattattc cacctatcaa
    22141 agcttccatt attgaattac ccataaatag tactccacct atgcttttaa ctataaaagc
    22201 cattatccat gctagtataa attgatatgc aaatgatgta aacatcattt tattgccata
    22261 ttcttttttc atagttgcaa gtgcagctat acatggagta tataaagcag taaatatcaa
    22321 gaatgtatat gcagtaactc cattaaatat tgttggtaag acaacagtta gattaccata
    22381 tagtatattt aaagagttta ctacgatttc ttttgctcca agaccactta aaatagcaac
    22441 tgaagttctc caatcatcaa aaccaagagg tttgaatata ggagatataa tatgacctag
    22501 agtagccaag aagcttacat ttatatcttc tgtatatcca aaaacattga atgaagaaag
    22561 tccccaaatt acgactgaca ttgcaaacat gacagtaaca actcttatca agaaaccttt
    22621 tgatttattc caagtatttt taataagtgc atctatagta gggattctat attctggtaa
    22681 ttctaatata aatggttcag cctctgtttt aaaggctgtt ttatttaaga ataatgcaac
    22741 tacaattgcc ataactatac ccaccaaata cagtgacgta gttactaaag cagcattatg
    22801 tggaaagaat atagctacaa atagtgcata tataggtagc tttgctccac aagtcataag
    22861 tggtgcaata agagctgtaa ctcttctatc tttttcgctt tccaatgttc tagttgccat
    22921 tatagctgga gaagaacatc ccatacccat aactataggt ataaaagctt taccagatag
    22981 accaaccttt ctcataactt tatccattaa aaatgctgtt cttgacatgt atccactatc
    23041 ttcaaacaac gatataccaa agaataatgt aaatattaaa gggaagaatg gtaaagtacc
    23101 accaacacct ccaattattc catcaactat aagtgattta aaccatgggc tagagttaga
    23161 taacatatca ctaacaggtg cagaaatata agcctctata agactagcaa atccctcttg
    23221 tagtggtcct ccgacccagt caaaggtaaa tttaaataag aggaacaata caccaataaa
    23281 tatcggatat gctaatatag gattcaagac tatattgtct atcttatcac tgatagattt
    23341 cttttgtgta tatgtagttt ttgtacatct cgataaaatg gattctaatt ttttatatgt
    23401 ttctgtttca cttccaaagt ttttctgata tataaaagct ttttgcgtag ctcccttaat
    23461 ctcttcttct atcctatcaa caccattttt cttttttgct ataattggaa caacaattac
    23521 tccaagttct tcacttaatt ttttggcatc tatattaact cctttagatt ctgcaatgtc
    23581 taacatatta agcaatataa ctataggctt gttaaattgc ataagttgag ttgttaagta
    23641 aaggtttctt gataaattag aagcatcaac tacatttact attacatcaa catcttcatt
    23701 ttctaaatat gatttagaaa ccttttcttc atttgagaaa gtatccattg catatatacc
    23761 aggtaaatcg acaaccttta tctcttttcc aagaaatcct tctttttttt ctatagtaac
    23821 accaggccag ttacctacat attgatttga acctgtaagt agattaaaaa cagtagtttt
    23881 accgacattt gggtttccta ataatgctac attaatcacg ggtagctcct cctctattat
    23941 tgtaaagaaa tatttttagc atcagatttt ctaatcgcaa ggtcaaagcc tctaaatcta
    24001 attataattg ggtctccaag tggggcaacc tttttaactt ctatttcagt actatttata
    24061 catccaagtg caagcaatct tttagataat ttaatatctc catctatact attaataaat
    24121 cctttttctc ctatttttaa atcgtaaaca gtcattatta acacctccta atgaaattca
    24181 ttatcattaa aattgtaaca agtatgatac aaaaagtcaa gatttataga ggtaattgca
    24241 cattgaaata actaatattt attctaaata gattaaaaat attttagtct aaattaggtg
    24301 tttttatata ttttgtatta taatgtaaga tgatagataa aatttggagg agttaactta
    24361 atgaaaacta gaaaaatgac atttttaggt cttatgatag ggtatagttt agctttatat
    24421 gtactagaga catatatacc aaatcctctt atagttatat ttccaggggc aaaattgggt
    24481 ttgagcaata ttataacttt aatttcatta atgttgttag gtgtcaaaga tactgttata
    24541 attgttacta tcagggtaat tttgtcatca atatttgcag gccctttatc atatctacta
    24601 tttagtatag gaggagctta tctgagtctt ttatttatgt atttagcgag taaaataaaa
    24661 gatttatcac taatagggat aagcattata ggtgcaatag ggcataatat aggtcaatta
    24721 atagttgcaa gtttaatcat agaaaatata cttgctatag ggtacttacc atttatgtta
    24781 atggcatcat tagtaactgg tatgtttgta ggcttagtat ccaggtatac aatacctaaa
    24841 ttaaagcatt tttatagtaa gttttattga cataatattg acaaaatata atattgtttg
    24901 ataaaatgta tataaaaata tacaaatttg taatagtggt gattattatg gcgaacaatg
    24961 aattagaagc gttaaaaaat gagattgaag cagttaggga agagataaat acatacatag
    25021 aatatccaga aatatttcaa gaagagttag tagaatcaag taagaaaata gatatattaa
    25081 taaataaata catgtaccta agcaaatagc ccacagttgt gggttttttt gtatcaaaaa
    25141 ttaacagatg gctatatttt acatatatta ataataatat taagatataa aatagtattt
    25201 ctgatgtgta aattatctgt aggacataag ttgtaattaa gtcaggaggg ttttatatta
    25261 tgattgatat aggaagtaag gagatagcaa taaggttact attagcacta ataataggag
    25321 gaataatagg cttagagaga gaaaaaataa gacagtttgc aggatttagg acacatatac
    25381 taattgcaat aggttcttgt attgtttcaa taacttcgat tcaattattc tttgattata
    25441 atttgtatac aaatgctgac ccagcaagaa tgcctgctca agtattatct ggaattggtt
    25501 ttttaggtgc aggagcaatt ttaaaaaata aaggtggagt aaaagggctt actacagctg
    25561 ctgggatgtg ggcaactgca tgtattggta tagcagttgg atatggttat tatgaattgg
    25621 caattctagg tggattattt gtaatgatag cattatttgt gcttaggata atcaatacgt
    25681 ttttatttag ccaagaaaag aatgtattga tattaaaatt aagcaatttg gactctatac
    25741 cttttttata tgataaattt gaaaaaaatc aacttatcgt aagaaatatg gaaatagaat
    25801 gtcgtaatga agaatactgg aaggttaatt tttttgtatc ttatgctaag agaatacact
    25861 tacacgacgt tatgaaagaa ataaatatga tagaaggagt aataaatgta gattatttgg
    25921 actaaaatat atacaatata ttttgtaaag tgaataaaat ataaaacaaa ttaaaataga
    25981 ggtgtatgat atgggaactg atagaagtac aatagaagaa aagtacaaat ggaagataga
    26041 taagatgtac tccagtaaag aggaaattga aaaagacata agtaaagtaa aaaatcttat
    26101 ccaagaagta aaagaatata aagggaagct ttctgagagt aaggagaact tatataaagt
    26161 attaaatatc tctgaaaatg catctaggat tgttcaaaat ttatatgtat atactcatat
    26221 gaaacaacat gaagatacta gaattaatga taatcaagga agagccacta agacagaaat
    26281 gttatctact gatttaggtg tggctacttc ttatatagtt ccagaaatac tggcgataga
    26341 agagaataag ttaagtgaat acttaaaaga tgagaagcta tccttttata caaaatatat
    26401 aaaggatata ttgagagata aaccacatac tttgagtgaa cgagaggaag aaatacttgc
    26461 agctacatca gaattatcga caattccaga aaatgtatac gatatgttag catatgcaga
    26521 tatggaattt cctgagatag aagatgagga gggtaataag gttaagcttt ctcattcaaa
    26581 ctactcttta tttataaaaa gtaagaatag tagagttaga aaagacgctt ttgaaggtga
    26641 attttcaaca tatgagaaat ataaaaatac atttgcctca acgctttatg gtggaataaa
    26701 atcagagata ttttacgcaa aaactagaaa gtataattca gcaatagaag cgtctttatt
    26761 ttcagatgat gtaagtttag atgtttacaa taatcttata tcagctatag ataaaagtat
    26821 tcctaatcta aataaatatg ttgagcttaa aaagaaattt ttaggattga atgaaataca
    26881 catgtatgat ttgtatgttc ctttgacaga taaatttgac atggatatac catatgataa
    26941 agcacaggat atcatattag aggcacttaa acctttagga gaaaaatact tgaaggtcat
    27001 aaaaagtgct tttgatgaag ggtggattga tgtatatgat aatgaaggta aaaaaggagg
    27061 agcttattct tggggaagtt atgattcaca tccttatata ttaatgagct ataaaaatga
    27121 ccttaattct ctattcactt taattcatga attagggcac tctgttcaca gtcattattc
    27181 aagaacaagt caaccatttt tatattcgga ctataagata ttcgttgcag aagttgcatc
    27241 tacattaaat gaattacttc ttataaatta tttattagaa aattctaagt ctaaggatga
    27301 aactatatac ttattaaatt attatttaga acaatttaga actacagttc atagacaaac
    27361 tatgtttgct gaatttgaaa aaattgtaca tcaaagggta gagagtggag aaccacttac
    27421 tgctgatgaa tttactaata tatactacaa attaaatgaa aagtattacg gaaaatctgc
    27481 aatagtagat aaacaaatag gaatagaatg ggcgagaata cctcattttt attcaaactt
    27541 ctatgtatat aagtatgcca cagggttttc tgctgcaagt gctttaagtc aacagatact
    27601 ttcagaagga agtactgcag tagatagata tataaacttc ttaaaaagtg gagggtcaga
    27661 atacccacta gaacaattaa aatctgctgg tgtagatatg actaaaaaag aatctataga
    27721 ggaagcctta aatgtatttg ctgaattagt taataaacta gaaaaagaat tataggatgt
    27781 gataggttga tagcttcaat gaagacttct gataatatga gttgtattgt gtttatatct
    27841 gcctaaagta actttaaaaa gttaatctaa ttaagaataa atcatataaa attaagtttt
    27901 tacatgtatt taacaatatt tacatttact taaccctgct ctaatattca tttaacaaag
    27961 aatctgtatt attaaaatat agaaaaatga caacaaaaaa ttaaaatata agcgggggta
    28021 agtaaaatgt ttaaaaagaa aataggcgtt cttttagcta gtacaatgtt agttggagct
    28081 ttagcagtag gatgtagttc taactcagga ggttcaggtg attcaggaga ttcaagcaaa
    28141 gtttctgttt caggttcaac ttcaataggt cctcttatgg aaactatcgg agaaaagttt
    28201 caagaaaaaa atcaaggtgt tagtgtagaa gtacaacaga ttggttcttc cggaggtata
    28261 aaaaatgcca tagaaggaac atctgaaata ggaatgagtt caagagattt aaaagatgaa
    28321 gaaaaaagtg ctggattaaa ggaaactaaa atagcaatag atggtatagc attgataact
    28381 aatactgcaa actcagtaaa aaacctgaca atggaccaag ttaaaggtat atatacagga
    28441 aagattacta actggaaaga agtaggagga aatgatgcac caatagtaat agtatcaaga
    28501 gaagaaggtt caggaactag agatggattc caagaaattg tagggtttga ttcagaagaa
    28561 ctaactaaag atgcacaaat aagtgatggt tcaggaaata taaaaactac agttgaagga
    28621 aatgaaaatg ctataggata tatatcattt ggatatgttg atgagaaagt aaatagttta
    28681 aaaataaatg atgttgaagc aactgatgca aatgtaatgg ctaataaata tccaatatca
    28741 agaccattta tattagttaa taaagaagat ggtttgtcag aaaaagggaa agctttaata
    28801 gattatatat taagtgatga aggacaagca cttgtttcag aaaaaggatt tattaaagtt
    28861 aatcaataac atgaattata atataaagta aacatataat accctgtgag ataagctcat
    28921 aaaaatgatg ctttaacttg cagggtattg tctattattg aatgtagaaa tataaatatc
    28981 tttagataaa ataggtattt tagtatctta tatctaaaaa tatggaaata ttgacaaaat
    29041 aatattacaa tgttagaata aaaaagaaat ttatgaaaat atataaaaga tttttaataa
    29101 aaatatttta gaaactgaga agtatatctt attattgggc atctggagat atatggagtt
    29161 agtggtgcaa ccggctatga atataatttt tatattgcat agtctttttt tgctttaaaa
    29221 ttaacaaaaa tgttgcaaaa tttgaaaata agagtattag tcgttaagat ttttattgat
    29281 aggtgaaatt ttggctttta aagtagccat ttgttggaaa agggaa
//
LOCUS       DACTGW010000027        29273 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.027, whole genome shotgun sequence.
ACCESSION   DACTGW010000027 DACTGW010000000
VERSION     DACTGW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.027"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..964
                     /gene="dprA"
                     /locus_tag="I9573_000921"
     CDS             <1..964
                     /gene="dprA"
                     /locus_tag="I9573_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAT4795550.1"
                     /translation="NISLNIRKNIVKYRGYAYLENIKELLYKKAIKYICKYDKEYPEN
                     LKNIYNAPKLLFYKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVS
                     GLAIGIDAYSHIGCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNIN
                     STVAPYHFSNRNRIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQM
                     SRGCHKIIKEGAKLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKRE
                     GNLHIDSICDYTGIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            997..3084
                     /gene="topA"
                     /locus_tag="I9573_000922"
     CDS             997..3084
                     /gene="topA"
                     /locus_tag="I9573_000922"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAT4795551.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            3249..4034
                     /gene="codY"
                     /locus_tag="I9573_000923"
     CDS             3249..4034
                     /gene="codY"
                     /locus_tag="I9573_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAT4795552.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            4141..5406
                     /locus_tag="I9573_000924"
     CDS             4141..5406
                     /locus_tag="I9573_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAT4795553.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            5437..6207
                     /locus_tag="I9573_000925"
     CDS             5437..6207
                     /locus_tag="I9573_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4795554.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDTLAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            6316..6762
                     /locus_tag="I9573_000926"
     CDS             6316..6762
                     /locus_tag="I9573_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAT4795555.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            6780..7973
                     /gene="nifS"
                     /locus_tag="I9573_000927"
     CDS             6780..7973
                     /gene="nifS"
                     /locus_tag="I9573_000927"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HAT4795556.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGRVDLRQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            8027..8467
                     /gene="nifU"
                     /locus_tag="I9573_000928"
     CDS             8027..8467
                     /gene="nifU"
                     /locus_tag="I9573_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HAT4795557.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            8756..9835
                     /gene="mnmA"
                     /locus_tag="I9573_000929"
     CDS             8756..9835
                     /gene="mnmA"
                     /locus_tag="I9573_000929"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAT4795558.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     gene            10284..12923
                     /gene="alaS"
                     /locus_tag="I9573_000930"
     CDS             10284..12923
                     /gene="alaS"
                     /locus_tag="I9573_000930"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAT4795559.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            12948..13205
                     /locus_tag="I9573_000931"
     CDS             12948..13205
                     /locus_tag="I9573_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAT4795560.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            13215..15386
                     /locus_tag="I9573_000932"
     CDS             13215..15386
                     /locus_tag="I9573_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795561.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYGTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNVGYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIININMIIFEKNN"
     gene            15592..16017
                     /gene="ruvX"
                     /locus_tag="I9573_000933"
     CDS             15592..16017
                     /gene="ruvX"
                     /locus_tag="I9573_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAT4795562.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            16095..16382
                     /locus_tag="I9573_000934"
     CDS             16095..16382
                     /locus_tag="I9573_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAT4795563.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            16664..17122
                     /locus_tag="I9573_000935"
     CDS             16664..17122
                     /locus_tag="I9573_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAT4795564.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(17239..17976)
                     /locus_tag="I9573_000936"
     CDS             complement(17239..17976)
                     /locus_tag="I9573_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAT4795565.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            18199..19869
                     /locus_tag="I9573_000937"
     CDS             18199..19869
                     /locus_tag="I9573_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAT4795566.1"
                     /translation="MFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSFPE
                     DEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLSIG
                     LIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGIIYH
                     TGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLDEL
                     FAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLDIS
                     DDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISAHP
                     IPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPAHG
                     EYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGVGD
                     VGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAKDI
                     IKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            20075..20275
                     /locus_tag="I9573_000938"
     CDS             20075..20275
                     /locus_tag="I9573_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HAT4795567.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            20329..21492
                     /locus_tag="I9573_000939"
     CDS             20329..21492
                     /locus_tag="I9573_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAT4795568.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(21703..22125)
                     /locus_tag="I9573_000940"
     CDS             complement(21703..22125)
                     /locus_tag="I9573_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HAT4795569.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(22146..22982)
                     /locus_tag="I9573_000941"
     CDS             complement(22146..22982)
                     /locus_tag="I9573_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT4795570.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            23280..23876
                     /locus_tag="I9573_000942"
     CDS             23280..23876
                     /locus_tag="I9573_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HAT4795571.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLIYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
     gene            23906..24625
                     /locus_tag="I9573_000943"
     CDS             23906..24625
                     /locus_tag="I9573_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAT4795572.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            24627..25166
                     /gene="scpB"
                     /locus_tag="I9573_000944"
     CDS             24627..25166
                     /gene="scpB"
                     /locus_tag="I9573_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAT4795573.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            25328..25957
                     /locus_tag="I9573_000945"
     CDS             25328..25957
                     /locus_tag="I9573_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HAT4795574.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADINNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTKSYGNNS"
     gene            25911..26339
                     /gene="ytfJ"
                     /locus_tag="I9573_000946"
     CDS             25911..26339
                     /gene="ytfJ"
                     /locus_tag="I9573_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YtfJ"
                     /protein_id="HAT4795575.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            26587..27027
                     /locus_tag="I9573_000947"
     CDS             26587..27027
                     /locus_tag="I9573_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT4795576.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            27043..28494
                     /locus_tag="I9573_000948"
     CDS             27043..28494
                     /locus_tag="I9573_000948"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAT4795577.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(28585..29253)
                     /locus_tag="I9573_000949"
     CDS             complement(28585..29253)
                     /locus_tag="I9573_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HAT4795578.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSQYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT        11821 a         3059 c         5260 g         9133 t
ORIGIN      
        1 aaatataagc ttgaatatta gaaaaaatat agtaaaatat agaggttacg cttatttaga
       61 aaatataaaa gaactacttt acaaaaaagc gattaaatat atttgtaaat atgataaaga
      121 atatcctgaa aatttaaaga atatatacaa cgctccaaag ttattattct acaaaggtga
      181 cataggttta gttaataata attttaatat agctatagta ggttcaagaa aacctacagc
      241 ctatggaatt aattgtgcaa aaacaataag ttgtcaacta tcccaatatg gagttaatat
      301 agtaagtgga ttagctatag gaatagatgc atactcgcac ataggttgta tgagtggaaa
      361 atctaaaact atagcagtac ttggttcagg agtagacaat cctcttccaa aacaaaattt
      421 acatttatca aataaaatat tagaaaatgg gggattatta ttatcagagt ataatattaa
      481 ctcgacagta gccccgtacc atttttctaa tagaaataga attatcagtg gtttaagtga
      541 tggagtagtt gttgtagaag cagcaataaa aagtggtgca ctaatcacag tagattttgc
      601 tcttgaacat ggaaaaaatg tttttgcaat acctggtaat ataaattctc aaatgagtag
      661 aggttgtcat aaaattataa aagaaggtgc aaaattaatt gaaaatatag atgatatttt
      721 aaatgaatat aacatattta atattattga taaaaaaata aatcaaaaat atgataatat
      781 aagtctaaat gccaagtcta agcaaataat tgaagctata aaaagagaag gaaacttgca
      841 tatagatagt atttgtgatt atactggtat agaaataaag tatgtaaatt caataatcaa
      901 tgaattagta ctaaatgagt tagtagtaga aatgaataat aaaacgtaca gtttaaatgt
      961 ataaattaag attaggtagt gggggtgtat ggttttatgg ccaaaacatt agtcatcgta
     1021 gaatcgcctg caaaagctaa aactatagaa aaatttttag ggaaaagtca ttacacagtt
     1081 aaagcatcag ttggtcatgt cagagattta cctaaaagta aattaggtgt agatatagaa
     1141 aataattttg aacctcaata tataaatata agaggaaaag gcgatgtaat aaaagaatta
     1201 aaaaaagaag ctaaaaaatc taaacaagta tatctagcaa ctgaccctga tagagaaggt
     1261 gaagctatat catggcattt agctcatata ctaaatttag atgaatctga ggattgtaga
     1321 atagagttta atgagataac taaggatgca attaaaaaag caattaaaca tcctagaaat
     1381 attgatataa atttggttga tgcacaacag gcaagaagag tgctagacag acttttaggt
     1441 tatcaaataa gtcctatatt atggcaaaag gttagaaaag gtcttagtgc aggtagagtg
     1501 caatctgtta caacaaagtt aatttgtgat agagaaaaag aaataaaggc atttattcca
     1561 aaagaatact ggacaataga tgttgaggct aagacagtaa aagacaaaga tataactttg
     1621 aaattttatg gtaaaaatga tgaaaagatt gaattagaaa atgaagaagc tgtaaatgaa
     1681 atactaaaag aaatggaagg taaagattta aaggttgaga agatagaatc taaatcaaga
     1741 cgtaaatcag ctccaaaacc atttacaaca agtatgcttc aacaagaagg agcaaataag
     1801 ttatttttta ctactaaaaa aacaatgatg atagcacaag aattatatga aggtatcgac
     1861 atagaaggtg aaggtactgt tggtcttata tcatatataa gaactgactc taaaagaata
     1921 tctgatgaag caaaagaaaa agctaagggt tatatcgtaa acaatttagg agaaaactac
     1981 tataaaaaca ctggtgataa aaaagaaaaa aaggatacta aaaaggttca agatgcccat
     2041 gaggcaataa gaccaacctc agttgataga actccagaga gtataaaagc ctcattgagc
     2101 aaagaccagt acaagttata caatttaatt tggagaagat ttgtagctag tcaaatggaa
     2161 gattcagtgt ttgatatact aaatgtagaa tgtaaaatag gcgatattgt atttaaggca
     2221 actggttcaa aaatgaaatt tgatggttat accaaggtat ataatttttc agaaagagaa
     2281 gacaaaattt taccaagtat agaaaaagga gatatattaa atatagaaaa tatattgcca
     2341 gaccaacatt ttacacaacc accagcaaga tatacagaag ctagtttagt aaaaacacta
     2401 gaagaactag gtataggtag accaagtacg tatgctccaa caatagctac tatactgaat
     2461 agagagtatg ttgaaaagca aggaacaagt ctatgtccaa cagaacttgg aataatagtt
     2521 actgatattt tggaaaataa tttccataaa tttatagatg tagattttac tgctgatatg
     2581 gaaagtaaac ttgatgttat agaagaaggc aatacagatt ggaaaaagat tgtatcagaa
     2641 tcatatgcac cacttaaaga ggctattgaa gaagctcttg aaaatataga aaaagtaaat
     2701 atggatgaag aaactgatga gatttgcgag aactgtgggt ctaatatggt tatcaaatat
     2761 ggtagatttg gaaagtttat ggcttgtaaa aactatcccg attgtaaaac aactagacca
     2821 attatgaata aaattggtgt taaatgtcca aaatgtgaag aaggagatat tatacttaga
     2881 aaatctaaaa gaggtaaagc tttttatggg tgttctaatt atccaaactg tgactttgta
     2941 gcttggaata agccaacagg agagttttgt gagaagtgtg gttcatatat ggttgaaaaa
     3001 gtgactaaat ctgaaacaaa aacagtgtgt tctaataaag aatgtaataa agagacaaaa
     3061 gagcataatg acggaaaaga gtgaaaattg aaaaaaatca cataattctc ttgaaaaagg
     3121 ttccaatcta tgataatctt tcaagtgtaa acaactaata gaaaaataaa ttttcattaa
     3181 atgtgaaaat aatattagaa aatataaaag gttattaaaa ggcctaaatt tcatgaggag
     3241 atgattaaat ggcaagtgaa gtgttacaaa aaacaaggaa aataaataaa acattacaaa
     3301 caagtggtgg aagcagtgtc tcttttgatt tactggccgg agcattgggc gacgttttaa
     3361 gttctaatgt ttatgtagta agtgcaaaag gtaaagtact aggtcttcat ttaaatgatg
     3421 ttcaagacag ttcagttata gaagatgagt atactaagca aaagaaattt tcagatgaat
     3481 atactcaaaa tgtgttaaaa attgatgaaa cattagaaaa tttaaatggt gagaagatat
     3541 tagaaatctt tcctgaagaa catggaagat tacaaaaata tactacagta gttccaatat
     3601 taggaagcgg tcaaagatta ggaacattgg tactttcaag atattcaaat tcattcaatg
     3661 atgatgattt agtaatagct gaatacagtg caactgttgt tggtcttgaa atattaagag
     3721 caataggtga agaattagaa gaagaaatga gaaagaaagc tgtagttcaa atggcaatag
     3781 gcactctgtc ctactccgag cttgaagcag ttgaacatat ttttgctgaa ttggatggaa
     3841 aagaaggtct acttgtagca agtaagatag ctgatagagt tggtataact aggtctgtaa
     3901 tagtaaatgc acttagaaaa tttgagagtg caggtgtgat agaatcaaga tcattaggta
     3961 tgaaaggtac tcatataaga atacttaatg acaaacttac agatgaatta aaaaaattaa
     4021 aaaacaatca ataatctata ttttataggt ttagattaga aaaggtatct atttcaaata
     4081 gatacctttt ttagacgaat tattaaggta atgataagta tattaaaaag gagtgagaaa
     4141 atgcctttag cagataaaat aagaccaaaa acaatgaatg atgtaatagg tcaaagtcat
     4201 ataataggta atggaaaaat attgtctaag atattacaat ctaatttttt accaaatatg
     4261 atattttttg gtcctcctgg tgttggtaaa actacagtag cggaaataat agcagaaaga
     4321 agcaataaaa gcttctataa aataaatgct actaattcat ctttagaaga tataaaaaag
     4381 gtgatatcag agcttggaag tataaataat gtaaatggag tcctattata tatagatgaa
     4441 atacaaagtt ttaataaaaa acagcaacaa tcaatattag aatttatgga aaatgggagt
     4501 ataactttga ttgctagcac tacagaaaat ccatatcatt atgtatataa agctctttta
     4561 agtaggtcta ctgtatttga atttaaacct ttagaaaaac ttgatataga aaaaggactg
     4621 aaaagagctg ttgaagtatt aaatgaagat agctatatgg atattgagtg caataataat
     4681 gctattgaag atatagcaat tctttctgat ggagatatga gaagagcctt aaatattttg
     4741 gaagtggtag tctattcaac taaaccaaat aaggataata taacttatat agattcagat
     4801 gtaataaggg catctacatt taataaaatt ataaactatg acaaaaatgg tgatagtcat
     4861 tatgatattt taagtgcatt tcaaaaatct ataagaggaa gtgacccaca ggcatctata
     4921 cactatctag caagacttat aaaaggtggg gatttgataa gtatatgtag gagactttta
     4981 gtaatagcta gtgaagatat aggtttagct tatccaaatg ctattgtaat tgtaaaggca
     5041 tgtgtagact cagctatgca acttggattt ccagaagcga aaataccatt agcagaagct
     5101 actattcttt tggctacatc tccaaagtct aattctgcat gtatagcaat aatgaaagct
     5161 atggatgaac tggataaaga gtttgttaag gatataccaa atagtataaa ggatgctcac
     5221 tattctggtt ctaaagaaat aggtaggggt tgtggataca aatatcctca taattttaaa
     5281 aatcattatg taaagcaaca gtatttacca gatagcataa aagatagtat ttattacata
     5341 ccacaagaaa ataaaactga gaaaaatata aaaaaatatt tagaatatct aaatagtgat
     5401 ttttagactg aattttatgt atcgggggta gatattatga ttataaaact taactcaact
     5461 tatcacagta gagttatgaa atacttaaaa aaagaacctg agtacaattt gtttataata
     5521 ggggatattg aaagatatgg atatggaaat aattttttaa atatatgggc agacgtgggt
     5581 gaacatggtg aaataaaggc aatattactt aaatattttg aatttatgat gttttattct
     5641 gatggagagt atgatgttga aggtttttat aatctacttc gtaatactaa ttacgaagaa
     5701 atatctggga aaatatgtgc tgtagatact ttagcaaaga gattaggact taataattta
     5761 aaggtagttg atttttgcaa acttcaaact aaaaagtttt tgatagataa taattgtaat
     5821 gcaaaggtta agagaattag gcttggaaat ctaaaaaaga cagtgaaact ttatgactta
     5881 atagatgaat ttcacagtac aactttagag aatttaaaaa atggactaag gacaggtaga
     5941 ggatattgta tagagattaa caaacaggtg gtgtcaatgg caaagagtac ttcagaaaat
     6001 aggactcatg caatgataat tggagtagga acacatccta aatatagagc taaaggactt
     6061 gctacaaaat gcctaattaa actatgttca gagttactga gagaaaacaa aattccttgt
     6121 ttgttttatg ataatgaaga ggctggaaag atatataaaa aactaggatt tgaaaacatt
     6181 ggaaaatggg gaatttattc aaaataaaaa attaaaatta atcttgacaa aattagtagg
     6241 gtataataaa attatcttga aagtaattcc tactaaaaca gtatgaatta gatttctata
     6301 tagaggtgat aatatatgaa gttgtctact aaaggtagat atggactaaa agcaatgttt
     6361 gaattagcat taaatcaaga caatggacca gtatcattaa aatttattgc taagaagcaa
     6421 aaaatatcag atcagtactt agaacaaata ttttcatctt taaaaaaatc gggtctagta
     6481 aaaagtgtga gaggtgctca aggaggttat ttgctttcaa agaatgctga agacataaca
     6541 gtaggagata tacttgtagt attagaagga ccagttgcat tatctgactg tgtactagat
     6601 gaagatgtat gtgaaaactc aaacatgtgt gtaacaaaaa tagtttggga aaagatgaaa
     6661 aagggtatag aagatgttat agattcaatt actcttaaag atatgataaa tgactataac
     6721 aaaaataaat tagaaaatga tataacaaat attaaaaaat agatttggag atgaatgtaa
     6781 tggaaaaaag aagattgtat atggattatt ctgcaacaac acctattaaa aaagaagtat
     6841 tggatgcaat gatgccatac ctaacagatt attttggtaa tgcatctagt tttcacacat
     6901 tcggaagaga agcaaaagat gctttggata aagcaagaga acaagtagca gcccttataa
     6961 atgctgaacc tagtgaaata tattttacag caggtggttc agaaagtgat aactggacac
     7021 tagagggtgt agcttatgct aataaaaaca aaggtaatca tataataact tcaaaaatag
     7081 agcaccatgc aatacttcat acatgtgaat acttagcaaa acatcatgga tttgaaataa
     7141 cttatttaga tgttgatagc gaaggaagag tagacttaag acaactagaa gattcaataa
     7201 aagatactac tatacttata agcataatgt ttgcaaataa tgaaatagga actatacagc
     7261 ctattaaaga aatatccgaa atagctaaaa aacataagat attattccac actgatgcag
     7321 tacaagctac aggtaatatt ccagtagatg ttaaagaatt aggaatagat ttgatgagta
     7381 tgtcttcaca taaaatatat ggaccaaagg gtgtaggagc tttatacata agaaaaggtg
     7441 taagacttca taactttgtc catggtggag cacaagaaaa aagcaaaaga gctggtacag
     7501 aaaatatacc tgcaatagtt ggatatggaa aagcagcaga attagctaag gaaaatatgc
     7561 aaaatcatgt agaaacttta actcgtctta gaaataagtt aatagatggt gtgttagaaa
     7621 gaatacctta cacaagagta aatggtagct tagaaaatag attgccagga aatgcaaact
     7681 ttgcattcca atttatagaa ggtgaaggaa tacttttatt attagatatg ttaggaattg
     7741 ctggttcaag tggttctgca tgtacttctg gttcattaga cccttcacat gtacttcttg
     7801 caataggttt accacatgaa atagcacatg gttcattaag acttacagtt ggagatttta
     7861 caacagatga tgatatagat tatatactag agaacttacc aaaagtaata gaaagactta
     7921 gaagcatgtc accactttat gatgatgcga agaaacaagg tttagtaaaa tagacagaaa
     7981 gataaagcat aaaaaataga cataaattga gtaggaggaa aagataatgc aatatagtga
     8041 taaagttatg gaacatttta tgaatccaag aaatatggga gaaattgata atgcaagtgg
     8101 tgtaggtgaa gttggaaacc ctacatgtgg agatataatg aagatatttt tagacataga
     8161 tggagatgta ataaaggatg ttaagtttaa aacatttggc tgtggttcag ctatagcaag
     8221 ttcttctatg gcaactgaaa tgataaaagg aaaaactata aaagatgcat tagaacttac
     8281 aaataaagct gttgcagaag ctttggatgg tttaccacca gtaaagatgc attgttcagt
     8341 tttagctgaa caagctgtaa aagcagctct tatagattat gctcaaaaga acaatatcca
     8401 tataccagaa ttggatggat atgttataga tgatgctcat gaccatgatg ttgaagaaga
     8461 agaataaaaa taagtgattg tgttatgaga agtttacaat gattaaaaag aagtactaat
     8521 gatataatta ggacataaaa aatagctgtt tttaaaatgg ctatttttta ttgcctaaat
     8581 taagtatttt aacttgattt agaggttaat tagttaacaa aataatccat aagattcaaa
     8641 taataaagat gattattttt ttagaaaata gaacaagcta tattttaaat aagtacaata
     8701 cttgctctat atggtataat aataagtaat gcaaaagatg aggtgaaatt tgtttatgaa
     8761 taaaaaagta atgataggta tgagtggagg agttgatagc tctgtagctg catatctttt
     8821 aaaacaacaa ggatatgatg tcattggtgt tactatgaag ttgtggcaag atgatgatgt
     8881 tgttgaaatt gaaggtggtt gttgctcttt atctgctgtt gaagatgcta gaagggtagc
     8941 aaataaaata ggtataccat tttatgtttt aaattttaga gaagtgttta aagaaaaagt
     9001 aatagattat tttatagatg aatatttgga aggaaaaact cctaatccct gtatagcttg
     9061 taataaacat ataaaatttg atgattttta taagaaggct agacaaatag gttgtgatta
     9121 tgtagctaca ggtcactatg caaagataga aaaggatgaa agtacaggaa gatatttgct
     9181 aaaaaaatct gttacagata aaaaagacca aacttatgca ctttataatc ttacacaaga
     9241 gcaactagag catactttat tgccaatagg tgactatgaa aaagatagag taagagaaat
     9301 tgctaaggaa atggggatgg cagtacacaa taagcctgat agtcaagaaa tttgttttgt
     9361 taaagataat gactatgcaa attatgtaaa aaaacattct aaaaagcgta ttgaagaagg
     9421 tttttttgta gatactaaag gcaatatact tggaaaacat aagggtatat tatattatac
     9481 tataggccaa agaaaagggc ttggaattac ttttggaaaa ccaatgtttg taatagacat
     9541 aaatccaata aataatacca tagttcttgg agacaatgaa gatttattta aaaaggaact
     9601 tattgctaaa gatgttaact ttatatcaat agatacgtta gaagaaccct taagagttca
     9661 agccaaaata agatattcag caaaaccatc accagctact attcatagag taggagaaga
     9721 tactattaaa atagtttttg atgaagctca aagagctatt actaaaggtc aatctgtagt
     9781 aatgtatgat ggagatattg tagtaggtgg aggtataatc gagaaaagtt tataaaaaca
     9841 tctaaaaaat taaaaaaata agcaataaag tatattaaga gtaaataaag tttataaaat
     9901 aaatttattg tatattaaaa tatagtattg attaattttg taaaatttag taatataatt
     9961 agtatattaa ttaaaaaaca aatacagtga taaggaatag taaaatattt gatgtcttac
    10021 agagagaggg aaatgctgga agcccttaga atatgatatt tgaagcagcc ttttagtagc
    10081 aatcttgaac aatttatctt ttataaagaa gatttaagta gagattgacg gtagttacgt
    10141 tatagctaag gttttccgaa gatatcatac tttgtatgat aattagggtg gtaccgcgat
    10201 tgtcctcgcc cctataaatt tatttatagg gacgaggttt tttatttaaa cgaaaaaata
    10261 aaatttatta ggaggaacat gatatggaaa agatgggatt aaatgaaata agaagtaagt
    10321 ttttagaatt ttttgagtca aaaggtcatt atgtagcaaa tagttattct ttagtaccaa
    10381 acaatgataa gagtttgtta cttataaact caggtatggc acctttgaaa aactactttt
    10441 caggtgtaga agtacctcca agtgtgagaa tgtgtacatc tcaaaaatgt ataagaacag
    10501 gagatataga aaatgtaggt ataactgcaa gacatgctac attctttgaa atgatgggta
    10561 acttctcatt tggagattac tttaaaagag agtcaatcaa atggggatgg gaatttgtta
    10621 cagaatggct aaatatacca gaagataaga tatgggtgac tgtatatgaa gaagatgatg
    10681 attcttatga tatatgggca aaagaaatga atttcccaga agaaagaatg gttagacttg
    10741 gaaaagatga taatttctgg gaaataggta caggtccttg tggtccttgt tcagaaatat
    10801 attttgatag aggagaagaa tatggatgtg acaatccaga ctgtaaacca ggctgtgatt
    10861 gtgataggta tttagaattt tggaaccatg tatttactca atttgataga gatgaagaag
    10921 gaaattatag tctattagaa aacaagaata tagatacagg tatgggtctt gaaagaatgg
    10981 gatgtattat gcaaggcgta gacactatct ttgaagttga tacaattaaa tctatactag
    11041 aagcagttga gaagttaaca ggagtaaaat atggagagaa tccaaaaaat gatatatcaa
    11101 taagaataat aactgaccac ataagagctg taacattctt agttagtgat ggagtgcttc
    11161 catcaaatga aggaagagga tatgttctta gaagactcct tagacgtgct gctcgtcatg
    11221 gtaagttatt aggagtaaaa gaattatttt tacagaaact aatagatgaa gttataaagg
    11281 taaatgataa agcatatcca gttttagttg aaaaagaaag ttatattaaa aaagtagttg
    11341 gaatagaaga agaaaaattc aatgaaacta tagaccaagg aacagaaata ttgaactctt
    11401 atatagaagt attaaaaaat gaaggaaaga ctgtcctaag tggtcaagaa gcttttaaac
    11461 tatatgatac ttatggcttc cctatagact taactaaaga aatattagaa gaagaacatc
    11521 tatctgtaga tgaagaagct tttaatgaag aaatggaaaa gcaaaaagaa agagctagaa
    11581 atgctagagg aaatatggat ggagaaagtt ggaaggaaga ccctctatca aaattagaat
    11641 ctacagttga cagtacattt aatgggtata gtgaaattta tggtgaagga actattgaag
    11701 ctatagttaa agatgatgag ctagttcaaa gtgcagaaga aggcgataag gtatctatag
    11761 tactagataa cactactttc tatcctgaag gtggaggaca agttggagat tgtggattga
    11821 taactaatga aaacttagtt ttagaagtat taaatactaa aaaaggtgct aataatagta
    11881 taaaacatat aggtataata aaatcaggaa gaataagtaa tggggacaaa gtaaagacac
    11941 tcgtagatag agaaactaga atgtcagctg caagaaatca cagtgctaca catttacttc
    12001 ataaggcatt aagagaagta ttaggagaac atgttaatca agctggttct ttagtaactc
    12061 cagaaagact tagatttgat attactcact ttgaagcgat atcaaatgaa gaattaaaag
    12121 taatagaaga aaaagtaaat aatgttattt tatcatcttt agacataaaa tgtgatatta
    12181 tgaatataaa ggaagctaaa gaaaaaggag ctacagcact atttggtgaa aaatatggtg
    12241 atgaggtaag agtagtttct atgggagatt attctacaga actttgtgga ggaactcact
    12301 tgacaaatac ttctcaagta ggaatgttca aaatattatc tgaaggtgga gtagcagcag
    12361 gtgtaagaag aatagaagca ataacaggaa aagctgttta tgaataccta aaagaaagag
    12421 atggaattat ctctgaagtt tgtgtaaact taaaatctaa agaagataat ttaattcaaa
    12481 gaataagttc tttattagaa gaaaataaaa acttatctaa agaattgcat gatatgaaag
    12541 ctaagatgag tttacaatca gttgattcta tttttgattc taaagttgaa gtaaatggag
    12601 ttaatttgat aactaataaa tttgaaggta tggatatgga tacactaaga gaaacagcag
    12661 ataatctaag agataagctt ggttctggtg tagtagtact tgcaaatgta gtagatgata
    12721 aagttaactt tgtagttact gccactaaag atgtattaga taagggaata cattcaggta
    12781 atattgttag agaagttgca aaaatagctg gaggaaaagg tggaggaaga cctaatatgg
    12841 cacaagcagg agctagtgat gtatctaagg ttgaccaagc tttaagctat gcaagtgagg
    12901 ttattaagac acaagttaaa taacattcac tcaaggaggt ttttagtttg gaaaaagatt
    12961 tagactacac tatgaaattt gaaggaatac cagaagatag gatgagtgta ggagatacta
    13021 ttgattttgt atataaagca ttagttgaaa aaggatataa ccctataaat caaattatag
    13081 ggtatctcct ttctggtgat tctagctata taactagtca taaaaatgca agagctataa
    13141 ttaaaaaatt tgaaagagat gagatacttg aagaagtaat cacgcactat ctgaatagaa
    13201 agtaggtcaa ataaatgaga aaaaagatta tgtcgttgtt tgcaatagtc attatgattt
    13261 ttgtatcaac tgctagctat ggttttgctg aagaaaaagg caaggtcata tttattgata
    13321 tgaataggac caatcttgga agtatgatga atattccaat cttgaaaaat gagatagaaa
    13381 agagaggcta tgtagctctc atgaatataa gaggggatca aggtactgat gatagaaggt
    13441 cttatgcaag tatgggtgca ggaacaagag ctagtgtaac tactgaagac tatatcaatt
    13501 ttcaaaaagt agaccaagat actgctaaag cttttgaagt cgcaacaggt caaaaagcta
    13561 aagaaataaa tgatttaggc attaatagat ctataaatga aaatcttgag tatggagaat
    13621 atggagcaaa tttagggtca ttaggaaaaa ctttatcaga aaatggttta aagacatctg
    13681 taattggaaa tgctgatata attgaaaata atcaattagt taaaaataga aacttatgtt
    13741 tagtagctat ggaccaatat ggaaggattg attcaggtaa tattgataat attaataaga
    13801 aagattctaa aatgccattt ggaatatcta cagactacga taaattacta gatgaaacta
    13861 aaaaatccta taaaaatagt gatgctatat ttgtagaact tggagataca tatagattag
    13921 atatgtatag attaaatcta aatgataata cttatggtac aatgaagaaa aatattgtaa
    13981 aaaatataaa taaatatctt gaacaagtat tttctatggt tggagaaaat gacactgttt
    14041 atatagcaag tgcatttcca agtgacctag attataagaa taagagaaga ctttctccaa
    14101 taataaaatt tagtggagaa gggaaaggtc tactggaatc tgccacaaca agaaaggctg
    14161 gaattgttgc caatttagat gttggagtag atatactagg tacatttggt cttgaaaata
    14221 aagagatggt aggtagaagt tatactttag taaataaaga ggacaatgtt ggctatctaa
    14281 aatcacttta tgaaaggata gtgtctatat cttcaataag gtcaactatt ataaatggct
    14341 ttgtaggcgt aatatctgct tcttgggtaa ttgccatgtt tgcgattttc tttagaaatt
    14401 atatgccaca taaagagcgt gtattcaaag tacttaagga gtttataaaa ttaggcataa
    14461 taatgccact atcatttatg ttagctcctt tgatgaattt taaaactcca atttcaatat
    14521 ctttgggaat atttgcgact acgctaatat tatacttgtt aggtagaaaa ttatttaagg
    14581 acaatgatat aaagcacatg ggattttttg caacacttac tattttagtc atagttatag
    14641 attctgtatt tggaacatac ttaatgcaaa ataacataat gagttatgat gctgtcatag
    14701 gagctaggta ctatggtgta ggaaatgaat atgaaggtgt aactatagca agtgcagtat
    14761 ttggcttagc agtattatta cattataaga aggtatctaa attacttgtt gtaatttttt
    14821 cgttaataat acttataact agtgcatatc cttcaatggg tgcaaatgtt ggtggagcta
    14881 tatcagaatg tgtagcatat ttgttgttta taatgcttat atatgacatt aaaatagatt
    14941 ttaagaaagc tgtattatta gctgtttcag ctgtagttgt cgtttttgca tttgcagctt
    15001 tagatatact ttcaggtagt gaatctcatt taggtatgtt tgtacagaga attttcctaa
    15061 atggaccagg agagatatta caaacatttg gaagaaaaat tcaaatgaat atgcaactag
    15121 cacagacaag tgtatgggta aatattttac ttgtaggaat tggagtaatt gcagtactta
    15181 tatttaaacc atctagacac tttagaaaga taatgaataa ttttccaatc ttgtttaaag
    15241 ggtttattgc atctatggtt ggttgtataa ttactctttt agttaatgat tctggaatag
    15301 ttgctgcatc tacagcatct atatatatac ttataccact aataataata aatataaata
    15361 tgataatatt tgaaaaaaac aactaaaaaa ttttttactg ttcatatagg atttaaataa
    15421 aaaatgcaat atgattatta atgtaaactg gatgcttaca taaaaaatca tattgcaatt
    15481 tttatgattg attatgcaaa attctatgaa gcaggaagcc tgtgacctta gttatgagag
    15541 gttcagaata tgtatttaat ctatagaata taaatagata aaggaggaac tatgttagat
    15601 ggaagaataa tgggtcttga tgttggtgat aaaactattg gagttgctgt gagtgatttg
    15661 atgggattaa ctgcacaagg tgtaaaaaca ataaaaagag ttggcaagaa aaaagatatt
    15721 gaagagttaa aagcaataat aaaagaaaaa caagtaaata aaattgtatc aggattacca
    15781 aaaaatatga atggaacttt aggtccacaa ggagaaaaag taatcaagtt ttgtgaattg
    15841 gtaaaagcag aaacaggtat tgatgtagag ttttgggatg agaggctttc tacagtagcg
    15901 gcagagaggt ctttattaga agcagatgtt agtagacaaa aaagaaaaaa ggttatagat
    15961 atgttggcag cagtaataat tttgcaagga tatttagatt ttaaaataaa ttcataaaag
    16021 aactataaaa ttgaatactt atgatatata atataaataa taaaaataaa aataaatttg
    16081 aggtgaatga cattatggaa gaaaatataa taaatttaat agacgaaaat ggcgttgaaa
    16141 gccaatttga aataatatta acattagaag cagaaggaaa agagtatgca atattaatgc
    16201 ctttagatga tgaagaagct gaagaagctt taatctttag aattgatgag gatgaagaag
    16261 gtgaaatatt aatacctttg gaaagtgatg aagagtatga aactgttgta gctgtgtata
    16321 ctgctataat ggaagaagaa ggtttaaatt acgacgaaga tgaatcaaat ggacttaact
    16381 agataagtaa ttaagtttgt atataaaaat cagatattta ttatatttgc tttaattaaa
    16441 ccttattaat tgtaaaagca tttatagtat atatctgttt ttttgttttt tattaaaaat
    16501 accaaaaaat ttatgaattt tggtgaataa tatatctttt atgggtatat atttatatta
    16561 tgagaataaa ttaaatttat ttaaatgtct tgacaaaaaa tgaattatca attacaatat
    16621 tatttaataa agattatcaa ttgaaaatag taagggtgat gttatggcaa atacaatgga
    16681 tttattaaaa gacaagttaa aagaaactgg ttttaagatt acgccacaaa gaagagcaat
    16741 agttgaaata ttgcttaaac atgaccattc acatctaagt agtgaggaaa tctatgattt
    16801 agttagggtt gactgtcctg aaataggctt agctactgta tatagaacaa tgcagctatt
    16861 ggatgaaata ggattaatat caaaactaaa cttagacgat ggatgtataa gatatgagat
    16921 tagtctacat aaagaagact gtcataatca tcatcatctt atttgtaaaa attgtggaaa
    16981 gataatggaa gctaaagaag accttttaga caatatagaa aaagagatac aatcattgta
    17041 caaatttaaa atacttgatc atgatgttaa attttatgga ttatgtgacg agtgtaatgg
    17101 agtaagcgat tcagaagaat aaacttctat tgcttaatgt cgatggaaga atagtataga
    17161 acttttatat tctatgctat tcttccattt tttattataa accatctaca aaatgcaaat
    17221 atacagtttt caagatgctt atagttttaa tattgaaaat ataattaatc ctataagtac
    17281 aaaaatgaat gatttttcta tgaaattact aaatggtcct ccaacagtta aaaaacctaa
    17341 agaaattttc ttttcgctta gcggataaaa aattggtatt ccttgctttg taaataaatc
    17401 tcctaaaaac atatgacttg cataaccaat cgtaccataa aaagataaat tattaatatt
    17461 agtgattaat tctatctgtt ttagtaaaaa atatactatt atagttgcaa atatgctatg
    17521 agaaaaactt ctgtgcttta accagggaaa aattgacaac attagacaaa aaattgtaaa
    17581 atatatttca acttctatga tgtataagca tatagctaat agtataaata ttaatgatag
    17641 aaaaactttt cttagaaatg tgtgggttag ttttgcttca ataattataa ttgcaacaaa
    17701 tgtaattatt gcgcttaaat aaaaattatc atttatattt attgaaataa agaaaataat
    17761 aatattaaca gcatatataa aaattctata tatgtatttg gatacattgt tttttagaat
    17821 gaaatttgag accatagaat tagatttatc caaatcgggt agtacagcaa aagtagctga
    17881 aactaaaata tcaactaaag atataggaat tttaaatatt aaagatgttt ggatagctgt
    17941 taagattcct attgcacaat gagttttacc acgcataaat cctcctaaat tatccgatat
    18001 taaatcatat aatatttaga ggatatcaaa ataaaatagg tttgtcaaaa taatacgtta
    18061 aatgtatttt aaatatagct taaaatatga tacgaattat ggtacaataa gaataactgg
    18121 tagcgagtgc gtgatttgat attatttatg attgtagaac gtgttaaaaa attaaataga
    18181 cagaaggaga tgatgcaatt gttcaaaaag aacaccaaca agataaaggt aatggccttg
    18241 ggtggactta acgaagttgg aaaaaatatg actgttgttg agtataaaga tgaaataatt
    18301 gttatagatg ctggacttag ttttccagag gatgaaatgt taggagtaga tatagttata
    18361 ccagatataa cttatttagt aaaaaatagg gataaaataa agggtatatt tattacacat
    18421 ggacatgaag accatatagg agctcttcca tatatattaa agaaaataaa tgtacctgta
    18481 tatggggcaa gacttagtat aggtcttata caagtaaaac ttaaagaaca taaaatgaat
    18541 aatgttaagt taaatgttat aggtcctaga caagttataa agcttgataa tatggaagta
    18601 gagtttttga aaaataacca tagtatacca gatgcttact ctattgcaat acacacagac
    18661 caaggtataa tataccacac aggagatttt aagattgact tgactcctat tgatggagat
    18721 gttatggata tgcatagaat ctgtgagttg agcaaaaaag gagttctttt aatgctggca
    18781 gatagtacta atgcagaaaa accaggattt actatgtctg aaaagactgt tggggtaggt
    18841 cttgacgaat tatttgcaaa aggaaatggc agaagaatta tagttgctac ctttgcatcc
    18901 aacatacaca gattacaaca aataataaat actgctgaaa agtttaatag aaaagttgct
    18961 atatcaggtc gctcaatggt caatgtagtc ggagttgcaa aagaattagg atacctagat
    19021 atttctgatg acatgcttat agatttaaat gatatatgta agtatgaaga tagtgaatta
    19081 gtgataataa caactggttc acaaggtgaa cctatgtcag ctcttgctag aatggctttt
    19141 tcagagcaca aaaaagtaga aataaagagt ggcgatttag ttataatatc tgctcatcca
    19201 ataccaggaa atgaaaaatt aatatcaaga gtaatcaatt tcttatttga aaaaggagca
    19261 gaagtagtat acagtgatat agctgatata cacgtttctg gacatgcctg tcaagaagaa
    19321 ttaaaactta tacatgcatt agttagacca aagtttttta tgccagctca tggtgaatat
    19381 agaatgttaa aaagacatgc tgaaatagct gaacagcttg gtatggataa agaaaatatt
    19441 tttgtcatgc aaacaggaga tgttttagaa ttagataaaa actctgctaa ggtagcaaac
    19501 cgtatacaaa caggaaacat attagttgat ggtctaggag taggcgatgt aggtaatata
    19561 gttttaaggg atagaaagca tttatcagaa gatggtctta tgatagttgt agttactatt
    19621 tccaaggatg agggtaaggt cttagcaggt ccagatataa tttccagagg attcgtatat
    19681 gtaagagagt cagaagattt aatggatgga gcaaaggata taattaagaa tgttctaaat
    19741 gaatgtgaag agaaaaatat taaagaatgg gcttatttaa agaataatat taaagaaaac
    19801 ctaaaagaat atttatatca aaaaactaaa cgaaatccaa tgatacttcc tatcataatg
    19861 gaagtataat ataaaaaaac tataaatttg aaaattagac ctcttgtaat ttttaaagta
    19921 atatcaaaaa tccaattgat attatcttaa gaattacaag aggtcttttt aatactcatt
    19981 taaaaaaaat atatattaca taaaatacca attataacaa tcttttatta gtaaaaacta
    20041 cttatatata aattttactg ataggaggat tcttatggat gatgtttcaa gacaaaatgc
    20101 tataaaagct ttaaaacaaa caaaaatgga aatagcagga gagtatggaa tgaattatga
    20161 agatgctttt gaaataatag aaaatgcgtc taataaaggt gttttagaag gttactttaa
    20221 aaagcttgaa aagaaaaaaa atttaggtca aggtatatct aggcatttag aataaaaaaa
    20281 gttaaatgtg agaaaataag tttaaataaa ctttaagagg tgacatcgat gaaaaaatta
    20341 gctagtactg ctttggctat attaattgcg cttactcctt taagcttttc ttttgcaaat
    20401 aataaggaaa atgctgatgc caaccaacta aatatatctt caaaatcagc aattttgatg
    20461 gatgttggta gtggacaaat tttatatgaa aaggatgctc ataaaaagct tcctcctgct
    20521 agtgtaacta aggtaatgac tatgctactt atagtagaag ctttggattc aggcaagatt
    20581 aagcttgatg atgaagttca agtaagtgaa actgcatcta gtatgggtgg tagtcaaata
    20641 ttcctagagc caggagaaac tcaaaaggtt gatacattat taaaaggtat agctgttgct
    20701 tctgcaaatg atgcttgtgt tgctatggct gaacacttag ctggaagtgt tgaaggattt
    20761 gttgatagaa tgaatgctaa agcaaaagag ttaaatatga atgatactca ttttgcaaat
    20821 acaaatggac ttccagttgc aaaccactat acatctgctc atgatatagc tttaatgtct
    20881 agagaactat taaaacacga aatgataagt aaatacttaa ctacatggat ggataaggtt
    20941 gtagtaggga agaaacaagt tacagtaggt cttgcaaata ctaataaact tataaagcat
    21001 tatcaaggag caactggtgt taaaactggt tttactcaag aagcaaaata ctgtttatca
    21061 gcatctgcaa agagaggtaa tactcatcta gttgctgcaa ctttaggagc agagacttct
    21121 ccagaaagat ttaatgatgc ctctagcctt ttaaactatg gatttgctaa ttatgaaagt
    21181 gttaagttat gtagtaaagg tgataatata gcaacactta cattggataa ggctgatgaa
    21241 aacaaagtta aattggttgc taaagaagat ttaaatgctc ttattaaaaa aggaagctct
    21301 aaagaatttg agaagaaaat tgaaatagta aaaaatccta agatgccaat taaaaaggga
    21361 actgttttag gaaaaataaa gatatgtaaa gataaaaaag taataggcga agtggaattg
    21421 ataaatacta aggatataaa taaggcaagc tatctacaaa tgcttcaaag aataattgat
    21481 aatatgatat aaataattat cagatgaaat ataattataa tacacaaaga tatgattata
    21541 atacacataa aaaataccct tatgtagtta tttgccatag gggtattttt atttaccata
    21601 tagaagtatt ttataatctt atgattttta tagaaatata gaaaagttta tgtatcttaa
    21661 aatatcattt gatttaatat catatatagg gttgtttaac aattatctaa gtaaaggcat
    21721 actaccagta agtttattca ctttcttttt attcccataa attgctatcc caaaatactg
    21781 taactcatct tctggaacac tagcaatctt ttgtgtaaat tcttcataaa gattgcaact
    21841 ttgtgctaca tctgaaaaat ctacaacagt taaatcgtta aattccaaag tatataattt
    21901 tttacgtaaa tcttttatta tttctttgtt tccttttaaa ataggtacag gaatgtcaat
    21961 aattcctaag tggctattac ctgttttgtc aattacatct ggcccaacag tttctggggc
    22021 atgttttcct agtgtaattc ccattatagc tgctgtattg gatataattc ccattggtaa
    22081 attttcgtct attaccatga cacattttga atttttattt tccattttta tttctccttt
    22141 ttaccttatt taaagttgtt tgaatcatca tttataaata tattttgata ctgctttggt
    22201 gtaagaccaa taaaattctt aaaaaagttt gtaaagtgac tttggtctac aaatcctgtt
    22261 tgtatcgcag catctatagg ttctacgcct ttttctaaaa gctttttagc tttgtttacc
    22321 cgtatatttt ctaggtatct atatggtgta atacctttta atttcgtaaa attacgaagt
    22381 agagagtact tattcatacc tgctatagtg cttaattcgt caagtactat atgttcagca
    22441 taattatttt ctagataatc acatactgct tgaatttcta tatttgtatt ttccaagtta
    22501 gattgcaaat ttggctctgt atgttcttca attaattgct caattaggaa gaaaaaaagc
    22561 tcttcctttt taaaatccaa ttcttcttcc atgattatat agtgtaattc ttgtagtaga
    22621 ggtactagct cactgcgaaa tataacaggt tgattaaatt ttggtaaata atttttacct
    22681 gtaatttcaa agacggtttt tttcattatt tctggcttaa tattgataca tctgtaatca
    22741 agtactttat catctatttg ttcacaagta tgactgtcaa aagggttaaa aagtagcaaa
    22801 tctccagtac ttgtggtata ctctttattt ttacaagata aatatctttg acctttttct
    22861 ataaaaccaa ttacataatg ttcatgaaaa tggtttggga atttttgcat tatgccttta
    22921 aatctatatg cttcaatttt taattcccta tcaaagcata ctgtgcgttc ttcttgtctc
    22981 acttaggtaa cctcctttca tgttatacaa agtgtaacac atattttttt atacttcttg
    23041 tataatattg actaagagac aataatgtca attcatgtca atataatatt acacttacag
    23101 taggtgtaat tattatattt tcacaatgaa tatgatataa taataaaagt tataaaataa
    23161 aaagcaaatt ttaattttat gcctagaagt taaaattatt ataatgtaaa ataattttga
    23221 ttataagcta tttaattgtt ataataatat taaaaattta atttaggaga tgtacattaa
    23281 tgttaagtat gaatatagga gaaatagtag caagtttagt tggtatagct atggcaatat
    23341 caatacatga gtttggccat gcttattcag ctcacttatt gggagatgat acagctaaag
    23401 cttatggtag aatgacatta aatcctgtaa gacatgttga tccaataggt cttttggcta
    23461 tgtttatcct taagataggg tgggcaaagc cagtaccagt aaatccaaat aattttaaaa
    23521 actataagat aggaaacctg atagtttcac ttgcaggcgt tttttgtaat gttttgactg
    23581 ctatactgtg tgtaattatc aataagtatg tgcatatgta tgctataaat acaatagctg
    23641 gatatgtgct tatatatagt atgggatttg cagcttttaa cttacttccg atacctccac
    23701 ttgatggatg gggagttata tctacatttg taccatataa atggaatgaa tatttgtata
    23761 aatttgaaag tatgagttat ataattttat taattgcttt atttactggt atatatcaaa
    23821 taatactatc accaatatat acagtgttgc taaaaatagt atatttattt gtttaatata
    23881 ataaacagat taaagaggga ttgatatgaa gtataatata caattacaag tctatgaggg
    23941 accattagat ttactttatg atttaataac aaagcataaa atagatataa aggatatatc
    24001 tataatagat ataactaagc agtatctaaa ttatttgaaa atgctagata aaatggattt
    24061 agagataact agtgaattta taactatggc atcaaaactt ttagagataa agtctaaata
    24121 tctgctttat aagcaaaaag atgaagaaga agacccaaga atagaactta tggaaaagtt
    24181 agaagaatat agaaagttta aggtagcatc acaagatata aaagaaaata taacttatgt
    24241 taatgagaga ttttatagaa ataaagaaga gataattatt gatgacaatg tggatttaga
    24301 agatatatca attgaagcaa taaaaaatat acttccatac atatttaaag ttaaaacatc
    24361 acagatagaa aatgctaatg atgagaaact agacaaaata gtaaggaaga agataatatc
    24421 tgtggaagaa aagatattgt atataagaga tattataaaa gataagattg aagttacttt
    24481 tacaaatatt ataaaatcat atgaaaatga tgaaataata gctacttttt tatctatatt
    24541 agagcttata aaagaaaaag aaatagtagt agtacaagac atattttttg atgatattct
    24601 aataagaaaa agttcggagt gttaaaatga aaagagaaga tataaagtat attatagaat
    24661 ctgttatgtt tgcatatggg gaaccaataa gtataaaaga attaaactat ataataaata
    24721 aagaactttc atcaaaagaa atagagatta tgttaaattt acttattgag gagtacagag
    24781 aacaaaatag aggtattcaa ataatcaagc tagagaataa gtaccaaatg tgtacaaata
    24841 aagactatgc agaatatata aagaagatta ttgaaccaaa gaaaaagaaa agtttgagcc
    24901 aagctacatt agaaactctt actataattg cctacaaaca accaataaca aaggtagaaa
    24961 tagaggatat aagaggagta aaatgtgata aggtattaca aactttgttt gaaaatgaac
    25021 ttataagaga agctggtaga ttgaataaga tagggaaacc aataatttat aaaacaacag
    25081 atgaattttt aaagttgttg aatatagaaa gtcttgaaga attgccgcct atagaaaatt
    25141 atcaagaagt ggcaacaaat gaataaaaat ataaaatcac aataatttag tatgtaattt
    25201 taaattttaa aagatttaaa atgctctaag ttgttgtgtt tttttgtata aatataaaat
    25261 tataattctc ttaaaataaa tatggataat taagtataaa tagtaatttt ttgatgataa
    25321 tataaacatg gagcttaatg aattatttaa ttttaagtat ttaataatag ctgtaatttt
    25381 tattataagt gtaactatgt tgattttaat atctcatata aacatcttaa taatagctga
    25441 tataaacaac aaagatattt gtttaaagct taatataaaa tacatgttta atttaataaa
    25501 tataaataga caactttatc cagctgaaaa tagtaaaaat aatgataaaa aagaggggat
    25561 gaagaataat atagattcaa gtatattatt agctgatgac cttttgagca tttatagatt
    25621 attaaaaaag ataaaaatac atgaacttta ttcaaatatt aattttggaa ctggaaatat
    25681 tggcttaaca tcttctgtat atgtattgat aaatactttg tatggaaatt tatttaatat
    25741 gattgatgca gaaaaaatgt atttaaatgt aaatccagat tttacaaaag attatgtttt
    25801 aggaaatata aggatacata taaggccaag aataaaagct ttatttaata ttataataat
    25861 gattaataaa attatgaata aaaataaagg aaataaggaa ggtgatagta atgagagcaa
    25921 caggttcgat acaaagtctt atggaaacaa ctcttgaaac aattaaaggt tctatagatg
    25981 caaatactat tataggagac cctataaaaa cagatactac agtggttgta cctatatcaa
    26041 aggttacaat tggctttggt ataggaggag gagaatactc aaaaggatat gatgataaag
    26101 atagggaaat tgaattaaaa aatgaaaaat cagatacgaa ttttgctgga ggtagcgcag
    26161 gggctatatc tgtacaacca gtagcatttg tagtagttga aagtggagag actagaataa
    26221 tgagtttaga cagtaatatc aatctagtgg ataatatatt atctataact cctagagtat
    26281 tggagaaaat tcaaaatata tcacaaagta ataagaatca agataaaaac aatatgtaag
    26341 aataaaatat ttttaaatat gtatatagtt ttaagtaaag tagtggggtt agtataaatc
    26401 ccactatttt gttgtgtact ttaaacattt atatctaata agattttagt ttaaaacaat
    26461 ttaaatagat aataaagttg atatatagta taattataaa aagaaaagaa gtattatatt
    26521 atagatatat aaacgcattt gagatataag agggagttat aaatcattag ttttgaggtg
    26581 aatgttatga gcgataaaga attatctatt ttgttgttag aaagtatgaa taattttcat
    26641 aatttaataa agattattaa taacgaaaga tataaaaaag ataaggttct tacagaaaga
    26701 cagttctttg ccttagtaaa aataagaaaa catgataaaa tagagcttaa aaatctaagt
    26761 agagatttac atgtatctac ttctagtctg tgtatactac ttaataaatt ggttgagcaa
    26821 gagtatgtat atagggaaga agatagtaga gatagacgaa atacttttta tggaataact
    26881 aaaaatggag aaaagatatt ggataatgaa atactaaagt ttgtatctat aataagtgat
    26941 aaaatggatt gcttagatat agataataaa gataaattat ttacttcttt ggaagagtct
    27001 aaaaatataa tagaacaatt attttaaaaa ggagaattta ttatgaatat aaagtattct
    27061 aaagagatat taaaaagttt agaaaatgct ataacagtag tagctctatt tgagggagaa
    27121 gatgtttcta cgacatttga taaaataaat atgataagca gaaattttaa ttctaaagaa
    27181 aatagtatag atactgtaac attggttgaa aataacaact tggtaaatgt ttgtcttgtt
    27241 ggttttggta agagagagtt attgaataga gaaaaaatta gaatcatagg tggtaatttg
    27301 tgtaaaaaat taaaaaatat attgaaaaat gaagcatata agagtagtga tgttaatgta
    27361 cttaatctta atttaaatag tgatgaaata ggttcttttg tagaagggat attacttgga
    27421 aattataagt ttgataagta taagacaaaa agtaaggatt ttgaaagaaa agaagttgat
    27481 actataacta tatttactga aaaagaagtt gagtgtcaaa tagaaaaggc taaaatatta
    27541 gctaattcaa ctataatagc tagaaatcta gttaatgaac catcaaacgt aatatatcca
    27601 aaaacattag caatagaaac agttaaactt ggtgcagagt tcgggtttaa tgttgatgtt
    27661 tatgaagaag aaaaaataaa ggcattggga atggatgctt ttttcgccgt ttcaagaggt
    27721 tcaatcaata aaccaagatt catagtaatg agatactttg gagataaaga aagtacagat
    27781 atattaggtc tagtaggtaa aggattgact tatgatactg gtggatattc tttaaaatca
    27841 aatgcttcta tgctagatat gaagacagat atggctggtg ctgcgagtgt tataggtgct
    27901 atgtgtgcta tatctcaaag taaattaaag aaaaatgtta tagcagtagt ggctgcatgt
    27961 gagaatgctt tatcaggagg ttcatacaaa cctggagata taattagttc tatggcaaag
    28021 aaaactattg aagttttaaa tacggatgcc gaaggtagac ttacattagc tgatgcaata
    28081 tactacatta taaacaatga aaaagtaaca aaggtagttg atgtagctac attaactggt
    28141 gctgctttaa cattgttagg aaatgttgca actcctatag taacaaataa tgatgatttt
    28201 tattgtgaat tagaaaaagc tgccacttta tcaggagaaa gggtatggaa gatgccaatt
    28261 tatgatgaat ttaaagatat gataaaagga gaagaagcag accttaaaaa cactggtggt
    28321 aaaaatgctg gatgtataac agcaggagct tttattggtg aatttgttgg gaatactcct
    28381 tggatacata tggatatagc ggggacaagt acatcatcaa aatcaattgg atacaaagca
    28441 aagggggcta caggagaacc tgttagaaca ttatactatt tggcagaaat ctaaaaaaca
    28501 attataaaat ataatttata aagccacatg gatatttttg taatttaaga ttatctatgt
    28561 ggctttattt tttatttaag taaattattt actctttttc tttttctcaa gtataaaagt
    28621 atctctatta aataaaagag caggtatgta cataatacta gtaacaaaaa taaacgtaaa
    28681 aatagcagtt gtgctatgac ttaaaaaacc ataatagtat tgtgagttgg taaatgtgtt
    28741 tagtaaaaat atgaatgaaa aataaataaa acatatagct aagttatctc taagtgcata
    28801 tctgttaact ctaaattttc taacatcaac catatatata tctgcataaa ttatcaagca
    28861 cttagagaag ataaatataa aatatattaa gctaggaaat tcatagccca agtttttaga
    28921 aaaaatgagt tctccaactg aacaaactaa actccaagaa aaaaatatat taaaaagatg
    28981 atactgctta aaaattagta tggctataca cagatatata cagatataat tgattccaat
    29041 tggtaaagag tttagagtac tataattacc tgaagaaata agtaataatt gctctagagc
    29101 cataattatt acaagtaatg tacaaattat cttctcaact gtatagctat aaggtaaaat
    29161 atttttggtt agttttggac aatagtataa aaataatcca aatataaaga gacttacaaa
    29221 ttgttcacta gaaaaaagaa aaattttaaa catataacat tctctcctat aat
//
LOCUS       DACTGW010000028        29000 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.028, whole genome shotgun sequence.
ACCESSION   DACTGW010000028 DACTGW010000000
VERSION     DACTGW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.028"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..254)
                     /locus_tag="I9573_000950"
     CDS             complement(<1..254)
                     /locus_tag="I9573_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HAT4795579.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQ"
     gene            complement(307..666)
                     /locus_tag="I9573_000951"
     CDS             complement(307..666)
                     /locus_tag="I9573_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAT4795580.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            complement(720..1805)
                     /locus_tag="I9573_000952"
     CDS             complement(720..1805)
                     /locus_tag="I9573_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HAT4795581.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            complement(1976..3325)
                     /locus_tag="I9573_000953"
     CDS             complement(1976..3325)
                     /locus_tag="I9573_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HAT4795582.1"
                     /translation="MPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDIYALQDLRLAT
                     GKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENASDIHIEPFKE
                     HLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGRVDIKLGGNLI
                     DIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINKRAGVLLVTGP
                     TGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGLSFDVGLRAIL
                     RQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLLEMQIPPYLLN
                     ASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKGYFGRTAIYEI
                     LEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLEECLVVNEMI"
     gene            complement(3348..4028)
                     /locus_tag="I9573_000954"
     CDS             complement(3348..4028)
                     /locus_tag="I9573_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HAT4795583.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(4420..5499)
                     /locus_tag="I9573_000955"
     CDS             complement(4420..5499)
                     /locus_tag="I9573_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT4795584.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKATEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      5787..5902
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6077..7447
                     /locus_tag="I9573_000956"
     CDS             6077..7447
                     /locus_tag="I9573_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4795585.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYALGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDISGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(7712..8323)
                     /locus_tag="I9573_000957"
     CDS             complement(7712..8323)
                     /locus_tag="I9573_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAT4795586.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(8313..10676)
                     /gene="lon"
                     /locus_tag="I9573_000958"
     CDS             complement(8313..10676)
                     /gene="lon"
                     /locus_tag="I9573_000958"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAT4795587.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFNSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     ARKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTAVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            10856..11368
                     /locus_tag="I9573_000959"
     CDS             10856..11368
                     /locus_tag="I9573_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HAT4795588.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            11356..11883
                     /locus_tag="I9573_000960"
     CDS             11356..11883
                     /locus_tag="I9573_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HAT4795589.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(11954..13204)
                     /gene="clpX"
                     /locus_tag="I9573_000961"
     CDS             complement(11954..13204)
                     /gene="clpX"
                     /locus_tag="I9573_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAT4795590.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(13223..13807)
                     /gene="clpP"
                     /locus_tag="I9573_000962"
     CDS             complement(13223..13807)
                     /gene="clpP"
                     /locus_tag="I9573_000962"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAT4795591.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(13956..15242)
                     /locus_tag="I9573_000963"
     CDS             complement(13956..15242)
                     /locus_tag="I9573_000963"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAT4795592.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(15436..15906)
                     /locus_tag="I9573_000964"
     CDS             complement(15436..15906)
                     /locus_tag="I9573_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAT4795593.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(15906..17255)
                     /gene="rph"
                     /locus_tag="I9573_000965"
     CDS             complement(15906..17255)
                     /gene="rph"
                     /locus_tag="I9573_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAT4795594.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARAIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(17415..19439)
                     /gene="ligA"
                     /locus_tag="I9573_000966"
     CDS             complement(17415..19439)
                     /gene="ligA"
                     /locus_tag="I9573_000966"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAT4795595.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(19625..20563)
                     /locus_tag="I9573_000967"
     CDS             complement(19625..20563)
                     /locus_tag="I9573_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAT4795596.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(20665..21087)
                     /locus_tag="I9573_000968"
     CDS             complement(20665..21087)
                     /locus_tag="I9573_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HAT4795597.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSEKNGENIIRDILDGNISSECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            21275..22558
                     /locus_tag="I9573_000969"
     CDS             21275..22558
                     /locus_tag="I9573_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4795598.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSHSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(22636..23151)
                     /locus_tag="I9573_000970"
     CDS             complement(22636..23151)
                     /locus_tag="I9573_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAT4795599.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(23151..24527)
                     /locus_tag="I9573_000971"
     CDS             complement(23151..24527)
                     /locus_tag="I9573_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAT4795600.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(24552..25103)
                     /locus_tag="I9573_000972"
     CDS             complement(24552..25103)
                     /locus_tag="I9573_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAT4795601.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(25161..25376)
                     /locus_tag="I9573_000973"
     CDS             complement(25161..25376)
                     /locus_tag="I9573_000973"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795602.1"
                     /translation="MQIHRELSKSENKYMCTKKEEQKCSKCEICGVLIDDTDKVYNNT
                     YNLCKKCKRNISTEKIVKYINIYDESK"
     gene            complement(25345..26178)
                     /gene="fdhD"
                     /locus_tag="I9573_000974"
     CDS             complement(25345..26178)
                     /gene="fdhD"
                     /locus_tag="I9573_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAT4795603.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCASVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(26227..28371)
                     /gene="fdhF"
                     /locus_tag="I9573_000975"
     CDS             complement(26227..28371)
                     /gene="fdhF"
                     /locus_tag="I9573_000975"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(27955..27957),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HAT4795604.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKTWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(28622..>29000)
                     /locus_tag="I9573_000976"
     CDS             complement(28622..>29000)
                     /locus_tag="I9573_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAT4795605.1"
                     /translation="AKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILT
                     LQAFLYMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSG
                     IFTVITHYRMKNRIGRNIVLSEN"
BASE COUNT         8660 a         5268 c         3179 g        11893 t
ORIGIN      
        1 tcttgaattt gaatgttaaa gtataaattt ttatataata tttttgattt accaatctcc
       61 tcttttacaa atctaactac atcttctata tatttaatct tctcaatcga attactatca
      121 ttttttatat caaagttaat ctcttttata attgatttat ctataaaaac ttttttatga
      181 ctttttctta aaaatacaag ccttatataa tcatcattta tttctaagtt tagaattttt
      241 ttaattatag ccatctcctt cttaaataaa aataatttta aaacttacag caatcaatat
      301 aaaatattat tttggataaa attgtttaga atttatattt acgcttatat ctgttccgct
      361 atcatttatg acaagttcga atttacctga tgtactctga ggaactggaa cagtatttag
      421 gtatcccttt tccttcaatg tttctacact tagagagtct attgtctttt catcatcatt
      481 aattgccatg ctagctgcag ttactatatt agcagcattc gtatagtctg catttaattt
      541 ggcactttct tgaaccttac tatacttaac taatgctata ctagatataa cgcctaaaat
      601 agttactact acaatcattt caacaagtgt aaatcctttt cgttttttat ttatcaaatt
      661 tatcaattaa atattctctc cttttcaaaa gtttcatcta ataaaattta tttttctctc
      721 taaaaattag taattgcatc aaacatagga acaaccattg ctaaaataaa agtgccaata
      781 attaatccca taaatacaat tagcataggt tctataaatt tcattatttg ctctattttg
      841 ttatttaatt cattttcata aaatttattt attacattta gacttttatc tagtttccca
      901 ctttcttctc ctgtatttgc cattgaaata aataattttg gaaaaacatt tgctaaattc
      961 aaagatttac ctatgctatt tccttttttt atataattat tagaaatctt taacctttca
     1021 taaataaatt cgttgtctat aacactagct gacatgtcta tagcttccac aatttgtatt
     1081 ccactgctaa ttaaaacgga caatgccctt gaaaatcttg ttgtagctac taactgatta
     1141 atttttttta taaaaggtat tttgaatttt aacgaattaa tcaatttttt tatattatca
     1201 tttgttataa agaaataaat gcttcctatt gaaaacaata taaaactaaa tattatataa
     1261 gctaagttat ttgtaaccac tgtagatatt cccattaaaa ctctagttat taaaggtggt
     1321 tcaattccat tgttggtaaa aaccacctga aaattaggta ttacaaatac gaatataaac
     1381 aacattgata agatagataa tattataagt attattggat atattgaaat agttattatt
     1441 ttcattttta atttgtattc tttatcataa taattagata aatcactcat tattatatct
     1501 aagtttccac tagtttctcc tgcttttatc atacttataa agaatttaga aaatatacct
     1561 gttatttgaa atgattctgc tattgagtta cctttttgaa tatgatttga tactatacta
     1621 agcaaattct ttactttttt acttgactga ctctcaatga tttcaaatat ttttgtaatt
     1681 tcacatccag attccagcaa tatgcctatc tctttgcaaa ttactctaat ttctttatca
     1741 ctgattctat ttaatactaa ataaaacttt tctaaattat aaaatatatt tttagataat
     1801 ctcatgtact ataatacctt ccaaattata ttaattacaa ttaaataaac ttaatatttt
     1861 ctaatttaat gcatttaatt ttatttcact taaatataat ttatactcaa acatcttaat
     1921 taaaatagat taaataaaga caactgacca aacgaagttt aactatacaa ttcagttaaa
     1981 tcatttcatt tactactaaa cactcttcta atgtcgttat tttttcattt attagtcttt
     2041 tacagctatc ttcaaatgtt atcattccat tattttgtgc cacatcttta atagtactag
     2101 aatcctccat atttcttata catgtcttta tatcatcatt aatttctaag atttcataaa
     2161 ttgctgttct tccaaagtat cctttatcat tacatttttc acaccctaca gctaccttag
     2221 tatttacatc tccactaaaa ttatctttta tcataatctc atggctacaa tgattacaaa
     2281 cttttctaac caacttttga gatataacac ctataagaga ggcatttaaa agatatggtg
     2341 gtatttgcat ttctagtaat ctagctatgg aagatactgc atcattagta tgtaaagtac
     2401 ttataactaa atgacctgtt atagctgctc ttactgctat cttagctgtt tctgcatctc
     2461 ttatttctcc gaccattatt atatctgggt cttgtcttaa tatcgctcta agacctacat
     2521 caaagctcaa ccctactttt gaatttactt gaatctgact tatgccatcc atcttatact
     2581 ctacaggatt ttctatagtc ataatatttt tatttattcc ttttaaatca tttaataagg
     2641 aatatactgt tgtagtcttt ccactacctg taggacctgt cactaataat actcctgctc
     2701 ttttatttat tatacgattt atagatttta tagcttcctc tgaaaaacca agttctaatt
     2761 tgtcttttaa aaaagtatct ctattaagta tcctcaaaac tattttttca ccatagacag
     2821 ttggtattga tgaaactcta atgtcaatta aatttccacc taactttata tccaccctac
     2881 catcctgagg aagccttttt tctgttatat ccattgatgc ctttaatttt ataaccgtag
     2941 acaatgatgg atagacatca actaaaaact ttgatacttc ctttagttct ccatctaccc
     3001 tcattctcac tattaaatgt tctttaaatg gttctatatg tatatcactt gcattttctt
     3061 ttatagcttt ttctaaaaga ttttcaaata atttttttgc ataatcctca tcaagttcaa
     3121 agctattata atatttttca atcttttcac ttataagttc ttttttctcc ttcttcaata
     3181 taatttcttt acctgtagct aatctcaagt cctgtagagc ataaatatcc tcatcttcta
     3241 tttctacaac taaattatca tcttgaaact caactggaaa tacattatat ttctttgcaa
     3301 gtttttctgg tattaacatt ggcaaactgc tttcctctaa tataatatta atcacccttt
     3361 atatacttta atttattagg tatattcatt tttttcatag agatattttc ttcagttttt
     3421 atgcttattt tcattggata attggtttgt atgctatcta aatatttact acctttatct
     3481 acataattag ataatttctc cttatctcca atgcacttta tttcatatgg ttgtgctaca
     3541 ggaactccat ttatatttat atggcttcct gcaagtacaa cttctgaata ttgattaatt
     3601 ctttgaccat ttatttctat gtattttcct ccgtttactt ttaattcatt tataactttt
     3661 acaagaatct tattatagtc tatactatta gctatattcc ctgttttttc atctatagca
     3721 tctatactta atattatccc actacccttt acatctgaat atgatagact atctctaagt
     3781 gtttctattt ctgatatttc ttcagaattt ttttgatttt gtttcattga attaatttct
     3841 ttatctaact tgtctttctc tttggttaat tcttttatag attttcttgt catttttatt
     3901 tcttttctta ttgacttgtc tttgcttgta taaactgttt cttcattgct ataatctcta
     3961 aatatggttc caacaagtat acctaaaata gtacttgcta gtattataaa cacatattgt
     4021 tttttcatac tgtattgctc cttacaaatt tatttttata tgataatttt ctttaattaa
     4081 atattaaaca tttacatatg cttattatat caaagttata tattaaaatt catctttagt
     4141 atataatatc atacattgaa ctcctatcac taatttacca atatcaagta tgcattttta
     4201 gatgttctta aaattatata aactttcaat aaacattgtt gtgatattat aaaaaaacat
     4261 aaattaggaa aggaaataat aattatgaaa aacaaataat ggctttggag attattaata
     4321 tcatttttta gcaaaaaaaa gtaaaatggc atagcctcaa agctacacca atttactaaa
     4381 aattatatta ttattaacat taattttaaa catatacgtt tataagtttt cctcatattt
     4441 taaaattctt ttaattaact tatctcttaa tttccctact tcatcaaatc tcatattagg
     4501 actatagtaa ctttctataa tatcatgttt agacttggct ctcttaagat ttagtactgc
     4561 ccaacaaatt aaatcatcta aaactttttc atcaaattta atatcttctt catatttaag
     4621 tagtttttct ctatttaaaa actgatttaa atcaattgta tcttcttctt taaaagtcgt
     4681 acttatagtt atgcctatat ctaaatcagt taatagtaca gtttcaattt tctctggaat
     4741 aagtggataa tgataaactt ctacatttaa acctttttca gtagctttct tatatacttt
     4801 gtttaataat gtagttttcc cagttcctat ttctccatct aaataataga ctttctttac
     4861 atcttttaaa atgttttctg tataatctaa acaccctaca ggagttatag ctgaaccaaa
     4921 taaatgcttt tctcttttta gatttcctgt gcttttaact ccatcaaata acctttttat
     4981 aaattcttct gtgaccaaat ttactttgcc ataatccata caatcagaat atttttcttc
     5041 tatgtctcta aaaattggtt ctgctgactt taaaaactta aatgctcttt taaatgaatt
     5101 actaatatct ttatcataaa ctgatatttc ttgtttattc ttttctaatt cttctgtatt
     5161 ccagtaatca cctaaattaa ttatctcatc caaagctcca ggtagctttg ggtccatagt
     5221 atgtggggca gttgcatcca taagtaccac gcctaatttc tttataacta aaccatccaa
     5281 cgaacttggg tcagaaggac aatgatataa atcaatatca tatcctctat gcaaaaactc
     5341 tttataaatt tttttcataa gtgaggattt tcctactcca ggtccaccct ttaaacaaaa
     5401 aactcttttt acattttcag gaattatata gtcaaaaaaa gaatatgagc cttgtgatgt
     5461 atttccacct gggaatgcct tctttacctt tccttccata ttagcgcctc ctcataattt
     5521 acaatatctc ttaattaaga tattcatatt ataagaattt tattacagct ttctgatttt
     5581 gacaaattta aaacttacca tctaatacta ataacatatt aattttatct aattattata
     5641 gattctcata ctgtacctta ttctatctat aaatacaatt taagtgtcca tattgaaata
     5701 tttgtattgt aatacagctg gatattactt aaatccaatt gtttccatta taattttatg
     5761 ttaaaataat attacaaaat acatctgttt ttcttcataa acgggtgaaa ttccctatcg
     5821 gcggtaaaag cccgcgagcc ttatggcata atttggtcat attccaaagc cgacagtaaa
     5881 atctggatgg tagaagaaaa tagtatatga gtacctttat gtaattttac atgagtaatc
     5941 tatacaaatc cttcaactac cgtatttatt catgaaatta gacacattca aggtacctaa
     6001 tatacaggtg ctttttttgt tgtttatttt acaattatat cgtacttata aaatctatta
     6061 agattggagt gttatcatga aacaaaaatg gatagtattg attatcatct gtattggtgt
     6121 atttatgtct actcttgatg gaagtatact aaatatcgca aatcctacaa ttgctgctga
     6181 ctttaaaatt aacatgtcac aaattcagtg ggttgtaaca gcttatatgc ttgttgtaac
     6241 agctactatg ttatttttcg gaaaacttgg tgataaagta ggttcaaatc gtctatacgc
     6301 attaggattt tttatattta caataggttc ttttctgtgt agtatgtcaa acaatttatc
     6361 aacattaatt tcttcaagaa tctttcaggc agttggagca agtattttaa tggctactgg
     6421 gcttggtata gtttccaatg catttccagc aaatgaaaaa ggaaaagcta taggaatcac
     6481 tggagctgta gttggtatag gaaatatgag tggtcctgta attggaggta taattttaga
     6541 acattttgga tggccttcta tttttattat caatatacct attggtatta tagcagtctt
     6601 tcttggcatt aaatttctgc ctaaacctgt tttggatgaa caaaacaaga gctttgatat
     6661 ttcaggtctt ctacttttcg catcttgcac tacgcttata ttacttgcaa tgaatgagaa
     6721 gggtaataca agattgtatt taggtataac agcacttata atcttcttat tgttggcatt
     6781 aagagaagta aaatttgaac aaagctttat tgacctacct ctatttaaaa atagaaactt
     6841 tactgtaggc aatatcattg gagtagcttg ttattttcct caaatggcag tatcttttct
     6901 acttccattt tatttagaac aattaaaaaa tttatctcct atgatggctg gttatgtaat
     6961 gactgtacat ccacttatta tggtacttat agcacctatt gcaggttcac tttctgataa
     7021 acacggtgca aaaaacattc taacagcatc tttttcattt atgaccatct cactagttgg
     7081 aatggcactt ttaaaagcag attctccatt gtatctttta attgtatgtt tagttatttt
     7141 tggacttggg ctaggagcat ttagttcacc aaacaatagt agtattttag cagatgttcc
     7201 tcctcaaaaa caggggtacg gaggcagttt tcttgcaact attcgtaatt tatcatttgc
     7261 tcttggaaca gcatttttta gtagtttttt tgcacaaagt cttacataca atcaaaagtt
     7321 taaaagtcac acatcagcct atgttatagc tagcaatcag tcttattgga ttgcagcatc
     7381 agtatgtttt atagggctta tactaactgt tttctttatg agaaaaacag ataaatctat
     7441 ttcttaaatg ctatttattg ctgactacat aagtcaataa ttttttagtt gaactaattt
     7501 atactaattt tcccatacct tactaaaatt tattagtatt tagcaaaatt actgatattt
     7561 attgtttata aacaataagg atgccaaaaa aagttcatta aaactatttt aaggcatcct
     7621 atattttgct ttaatattaa tataattaaa actaactatt attacattta ttattcaaat
     7681 aaatcttatg tcaatattta acattttaaa tttaatctac tgttatttca tatccatgtt
     7741 gattgtattg atttacaatc tcttcccata attcttcaac accagtcttt tttagtgaag
     7801 aaacaggaat tactttttca ctactttcca tttgcaattt ttttcttatt atacttatgt
     7861 gtttttgata ttgtcctcta gatattttat ctgccttagt tgcaataact acacagttat
     7921 atccaaagtg ctttatccat tcatacataa gcttatcatc tgctgtaggc tcatgtctaa
     7981 tatctacaag taggaatata gaacataact cttgtctact ttctagatat ctttccatta
     8041 tacctcccca cttttctttt tcagacttag ctatctttgc atatccatag cctggaaggt
     8101 ctacaaaata aaattcatta tttattagat aaaaatttat agttctagtt tttcctggtg
     8161 tttgactggt tctagcgaag tttcttctgt taagcaatgt atttattatt gatgattttc
     8221 caacatttga cctaccagcc aatgctatct ctgggattcc ttctgctgga tactgtgatt
     8281 tattcacagc actcatagtt atctctgaac ttctaatttt cattattttc accactcttt
     8341 aataaagctt gttctaatac ttcatccata tgttctacaa gcacaaactc cattttttct
     8401 tttacatttt ctggtatttc atctaaatca gcttcacatt ctttaggaat taatactttt
     8461 gttataccag ctctatgagc tgctaataac ttttctttaa cccctcctac tgctaagact
     8521 ctacctctta aagttatctc tcctgtcatt gctatatttc caggaacagg tcttttagta
     8581 agtgctgaga ttactgcaag agccatagtt ataccagctg atggtccatc ttttggaaca
     8641 gcgccttctg gaatgtgtat atgaatatca ttcgtcttat aaaattcagg gtctatatca
     8701 aacttgtcca ctattgacct tatataagag atacctgtct tcgcagattc cttcataaca
     8761 tctcctaatt ttccagttaa tacaatttct cctttacctt taagtacgtt tacttctact
     8821 tcaagtgtaa ctccaccaac tgctgtccat gcaagaccat ttactaatcc tatttgagga
     8881 ttaacttctg caagttgata cttaaacatg tcttttccaa gatatgtctc taggtcagac
     8941 ttatttataa caacctcttc aagagttggg tcttcaacgt attttttggc aacctttcta
     9001 caaatcttac caatagttct ttctagtgtt cttacaccag cttctcttgt ataatggtta
     9061 attatacttc ttaaagtttc atcatctatt tttataaagt tttcttttaa agcatgttct
     9121 ttaatctgtt ttggaagtag gtattttctt gctatattta atttttcttc ttctatatac
     9181 ccagatactt ctattacttc cattctatct aataatggtc ttggtatatt acctagacta
     9241 tttgctgttg taacaaatag tatttttgat aaatcaaaag gtatttctaa gtaatggtcc
     9301 acaaagtcct tattttgctc tgggtctaat acttctaaca ttgctgaagc tgggtcacct
     9361 ttataatctg ctgccatctt atctatctca tcaaataaga atacaggatt ttttgtttgg
     9421 gcttctttta ccccattaat tattcttcct ggaattgaac ctacataagt tcttctatga
     9481 cctcttattt ctgcttcatc tctaactcca cccaaagata ttcttacaaa ttttctattt
     9541 aatgctctgg cgattgattt tactatagat gtctttcctg ttcctggagg accaactaga
     9601 catattatag gccctttaag tgacttagca agagttctta tagctagata ctctagtatt
     9661 ctctccttaa ctttttctaa tccataatgg tcttcatcta atatatcttt tgccttagtt
     9721 atatctaatt tatcttttgt ctctttattc catggcaatg agaatatagt atctaaatag
     9781 tttctactta cagatacatc tggagacata gaagatattt ttgaaaattt atctatctct
     9841 ttttctattt tttctttggt tgtcttaggt gcttttattt ttttaagctt ttctctatac
     9901 tcatctgctt cagaatttat atcttcttct tcacctaatt ctttctgtat agcttttaat
     9961 tgttctctaa gataatattc tttttgaacc ttattcatct gtttttttac tcttaaagtt
    10021 attttctttt ctatttttaa tatatctatt tcctctaata atatagaata aataagttca
    10081 agtctttttc taatatcaaa ttcttctaat atttcttgtt tttggcttga ttttagatat
    10141 atgtttgcag ctatcgtatc tataaatcta tctacatctt ctatatcagc tagagatatt
    10201 aatatttctg gtgataccct atttccaata tttatatact cttcaaaggc atcaaataca
    10261 tttcttacaa atgcttcaat ttcaacttct gtttcactat ccaaattatc actattaaat
    10321 actatttctt ctattactgc tctgaaatac ccatcttctt gttctatttt ctttactcta
    10381 ccccttgata caccttcaac caaaacacga actgtatctc ctggtaattt tatcatttgc
    10441 ttaactttac agattgttcc cacatgataa aaatcctctt ctcctggctc atctacctct
    10501 gcttctttct gagaagttaa aaatataagt tcttcatcca tcatagcttg gtctaaagct
    10561 ttaagagata tctctctacc tatatcaaaa tttaatatca tatatggaaa tatagctaat
    10621 cctcttagag gaattaatgg taattcatga tctattttag tataattttg ttccatcaac
    10681 attactcctt ttctagtcta attgctttgc ttttaatatt atatatttga acaagttaat
    10741 tttcaacatt aatgataaat tcttagaata ctatcatact atattatctt tttattatga
    10801 tataatatta atattagtat acttagatgt tatttatctt ttggaggttt ctattatgtt
    10861 gaaattgttt ttaacctttt taaaaattgg tgcgtttaca tttggtggtg gatacgctat
    10921 ggttcctctt atacaagatg aacttgtaaa caaacaaaaa cttttagagc aggaagaatt
    10981 tattgattat ttatccatag ctcaaagtta tcctggagta ctggcagtta atatatctgt
    11041 attattagga tataaacttt atggtatacc aggagttctt atatgtacct taggctctgc
    11101 tttaccatca ttttgtatag tcttagtctt atcgtatcta tactttaaaa attcatcatc
    11161 taaaatttta gatggatttt ttaaaggagt tgcgcctgtt gtaatagctc ttatattgta
    11221 ttcttttacg agcatgttca agaaactacc aaagtctaaa actaacatac tactattaat
    11281 tatagcagtt ttatctgttg gtgtattcaa tataagccct atttatttaa taattggagg
    11341 aggtgtatat agtctatgtc agaaataata ctactcttct taatattcct taaaataggg
    11401 gcatttagtt ttggtggcgg ttatgccatg ctaccattta tccagcaaga atttatatct
    11461 aaatatcact ttataactaa tcaagagttt ttggatttac ttgctatatc acaatctaca
    11521 ccaggtcctg tagcaataaa tagtgctaca tttgtaggat ttaagactgc tggcttcttt
    11581 ggttctctag ctgcaactat tggtgttaca atttttgcat taataggtct aactattata
    11641 tcaaagttat tttctaaatt taaaaataat aaaaaggtag aaaacttatt gacagttctt
    11701 agaccaatta ctctaggatt catattatca gcagcttttt catctgccaa agaagttaat
    11761 tgggatttat atggaataat tgcatttata gcatcatttt atcttttata taaaggaaaa
    11821 attggttcca ttgcaattgt attcgtcttt ggtattctag gtatattact cacattttta
    11881 tgataatgat acctatttta tatttagcat attaacataa aaaaaggagc ttttaaagct
    11941 ccttttcatc atcttatgca ctttcctctt gatccttaag tactatgata ggatttactg
    12001 agtcttcgtt cacagatttc tctgttacta taaccttctt tatattatct ctagatggta
    12061 cttcaaacat agtttccatc attacgcttt caactatact tctaagtcct ctagctcctg
    12121 tatttctttc tatagccttt ttagcgattg cacgaagagc accttcttca aattctagct
    12181 caacatcatc taactccaat aactttttat actgttttac tagagcattt ttaggttcct
    12241 gtaatatctg cattaatgca tcttcatcca acaactctaa agtagctaaa actggtattc
    12301 ttcctataaa ctctggtatt ataccatatt ttaataaatc ctctggaagt actttttcat
    12361 acagcttccc taaatctaat tcttttttac tttctatttt agctccaaaa cctagtgttt
    12421 tatctccacc acgtttttga attatcttct ctaatccatc aaaagctcca cctaatataa
    12481 ataatacatt agtagtatct atctttaaaa attcttgatg gggatgtttt cttcctcctt
    12541 gtggtggaac atttgcaaca gtaccttcta atatttttag aagtgcttgt tgaacacctt
    12601 caccactaac atctctagtt atagatgggt tttcagattt tcttgcaatc ttatctatct
    12661 catctatata tattatacct ctttcagctt tttctatatc aaaatcagca gcttgaataa
    12721 gttttaaaag tatattttca acgtcttctc caacatatcc agcttcagtc aatgatgtag
    12781 catctgccat tgcaaaaggt acatttagag ttcttgctag agtttgagca agtaatgtct
    12841 ttccagaacc tgtaggtcct aataaaagta tattactctt ttgaatttct atatccttac
    12901 tactagattt tttactgtat attctcttat aatgattata aaccgcaact gataaagctt
    12961 ttttagcttt ttcttgacct attacataat catttagaat ctccatcatt tcctttggtt
    13021 ttggtaaaga tgtagtatct tcatctatag tatcttctat ttcttcctga ataatttcgt
    13081 cacaaagttc aacacactcg tcacaaatat atacatttgg acctgcaatt aatcttctta
    13141 cttgatcttg atttttaccg cagaatgaac actttaattg tcgtttttct tcatatttag
    13201 acatattaac acctctctct atctacggtc tctttgtaaa tacttcatct attaaaccat
    13261 attctttggc ttctagtgca ctcatgaagt tatcacgttc agtatccatt ttaatttttt
    13321 ctaatggctg accagttctt tcagataata tctcatttaa agtttctttt atttttaaaa
    13381 ttctctttgc atgaatttct atatctgttg cttgaccttg agctccacct aatggttgat
    13441 gtatcattat ttcactattt ggtagtgcta gtctttttcc ttttgctcct gctgccaaca
    13501 agaatgctcc catagaagca gccataccta tacatatagt acttacatct ggtttgatgt
    13561 attgcatagt atcatatata gccattccag aagttatact tcctcctgga gaatttatat
    13621 ataaatgtat atctttgtct gggtcttcag cttctaaaaa taatagctgt gctactataa
    13681 gccctgctgt ggcatcgtta acttgatctc ctaagaagat tattctgtct tttaatagtc
    13741 tagaaaaaat atcataagat ctttctcctc ttcctgtttg ttcaacaact acaggtacta
    13801 atgccatggt ttcatacccc ctttattcta tataatttac agtagctttt ttaagctact
    13861 gtaaattatt atacgcattt aactaaatta tatttgcaca tcattatata ctaaacattt
    13921 cataacgtca actatattag ttagcgttta ttttattaag ctattgttgc attatcaact
    13981 aataaatcta ttgtttttct tatttttatt tgacctttta tatcttcttt gtcagcatct
    14041 cttaaagaag cttctatttt ttctacttcc atattgtaag cagaagccat tttttctaac
    14101 tcagccttaa attcttcttc tgttgcttca attccttcag ctttagttat tgcttctaat
    14161 actaaagaag acttaactaa ttttttagca tcttcttttc tctcttctct taattcttcc
    14221 atagttctac cagtcatctc tagtaattgt tgtaatccaa atccttgata ttgtaattgg
    14281 tagttaagtt catttaacat attatctatt tgatgttgta tcataacttc tggtatttct
    14341 atttctgtat tttcagcaac tttttctact actgaatttc tagtttctgc atctgctcta
    14401 tttttagctt cttcttctaa cttagctctt acatctgctt ttaattcatc taaagaatca
    14461 aattcactag tatctttagc aaattcatca tcaagtgcag ataattcttt tacttttaca
    14521 tcatttattt ttacattaaa tacaactggt tttccagcta aatcttgtga gtgatattct
    14581 tctgggaaag taacattaac ttctacttct tcaccagctt ttttacctac taattgttct
    14641 tcaaatcctg gtataaatgt gtttgaacct ataactaagt tataattttc acctttacca
    14701 ccatcaaaag ctacaccatt ttcaaaacct tcgaagtcta ttatagctgt atctccatct
    14761 tctaaagctt tgtcttctac acttactaat ctggcatttt tttcaaccat ttcttgtagt
    14821 ttagcatcaa cattttcgtc acttacagta ttctcaacct tagctatttc tatacccttg
    14881 tagtttccta attcaaactc tggtttaact tctacattaa gaaccataac caacccatta
    14941 tctttactta tttcttctat atctaaatct ggattgtcta ttgggtctat gtctaattcc
    15001 ttgatagctt ctggataaac ttctgggaat aatatttcta tagcatcatt gtagaatata
    15061 ccttttccat attgagtctc tataacaatt cttggagctt taccttttct aaatccaggt
    15121 atattgtatt tatttctgct tttattataa gccttattta ctgctgcttc aaatttattg
    15181 ttatctactg ttatttttaa agtaacttta ttaccctctt ttttaattag ttctgctttc
    15241 atccttctaa tcctcctagt gatatttatg taatacccga taacggtatt ataattcatt
    15301 ctatttataa ttcaaccact taattatatc acataagttt ttttatttac aagtgttcca
    15361 ttaatatcgt tgtacttatt tgataaaatt taattttttg agcgtttttt gccatttttt
    15421 tagataaata cacctttata tgcgtatttc ttcaattttt atgtcatctt cttctatatc
    15481 cataatgaca aaacttttat acgatactcc tcttggaagt gaagtgcttc caggatttat
    15541 gtaaagtaca ccatctttaa tttctctaaa tggggtatgg gtatggccaa atataactat
    15601 atcagcatca acttcagttg cctttttttc taacatatta gttccatatt ttacaccata
    15661 cttatctcca tggcataaga atatggtttt atttgccact tcaaagacaa cttcttcctc
    15721 aacagcatca aagtcacaat tacctttaac tgcaataata cctacattag tcatgctatg
    15781 aatatatctt gaatcagtaa agttatctcc tgcatgaatt attaggtcac attctttcaa
    15841 atatggtatt gttttttcca tatttttaat catcatatgt gtgtcgctta ctatgcctat
    15901 tttcattact tcactaccct ttttataaac tcttgtttca taagttttag tgcgtttgct
    15961 ctatgactga ttgaattttt tataacacta ggtatttcac caaaagtctt atcatatcct
    16021 tttactataa ataagctatc atatccaaaa ccatttttac ctttttcttc aaatcctatc
    16081 attccttcac aaattcctct tactacaaat tcttttccat caggaaaaac tactgctatt
    16141 gcagagacga atcttgcatt tcttttgctc attggtacat tttgcatagc ttttaacagt
    16201 ttttttctat tttcttcatc tgttgcatgt tctcctgcat atctagctga ataaacacca
    16261 ggttttttgc ctaaagcatc tacttctaag ccagaatcat ctgatattgc tataagttta
    16321 gtcttttttg ctattgctct agcttttatc aacgcattat gctcaaatgt ttttccatct
    16381 tctactatct ctatacctgc taaatcaaca tcttttaaag agtacacttt atattcaaaa
    16441 tcttttaaga tttcacctat ttcttctagc ttatgagcat tattagtagc aatcactact
    16501 tcattaccat aatccatacc caatatctca tctgctattt cacctaaagc ttctctttga
    16561 agccttatta attctttatt ccctttttcg cctaactcta acaataaatt taaatctgct
    16621 cttgaaaaag gtttctcttc tcctgtcccc tgtacttcta caaactcaca cttatcagtc
    16681 attattatat tcatatctac ctgtgcttta gaatcttctt cataacataa atctagcatg
    16741 tgtattccat ttacaatacc aacactaatt gcagatacaa aatttgttat tggcatctct
    16801 tttattgtct ttgctttata taatttatat attgcttctg caactgctac aaatgcccct
    16861 gttatagatg cagttctagt tccaccatct gcctgaatca catcacaatc tacccaaata
    16921 gttctttctc ctaatttatt caagtctaca acagacctta ttgctcttcc tattaacctc
    16981 tgtatttctt gtgttcttcc atcaattttt cccctagatg catctcttat ctttctttgt
    17041 tgtgttgctc ttggtagcat agaatattca gcatttatcc atccagtacc actatttctt
    17101 aaaaaaggag gaactttttc ttccactgat gcagtgcata taacctttgt ctctcccatt
    17161 tctataagaa cagaaccttc tgcatacttt gtataatctc tagttatctt tacaggtctt
    17221 atctgttcgt tacttctatc ataacttctg gtcatttttt atatcctttc tcatattttt
    17281 tattttttat aaatttattt ttataatttt taattttgtc aaatattaat tattgtcatt
    17341 tttcgtaatc ttattagctt actgaactat tctatcatta attatagtag ttttctacta
    17401 aattaagcga ctttttaact ctctaataca gcttttactt catctttcga tgataacttt
    17461 aataattcta aaaatctctc ttcatctata actttaattc ctaaatcatt tgctttagta
    17521 agtttagaac cagcttcttc acctgccaaa acaaatgttg tcgatttact aacactagat
    17581 gtagcttttc cacctctttt ttctatcata tctttcgcat catttctttt taatgtagga
    17641 agtgtaccag tcaatacgat tttcatccct tcaaatattt tttctacttt ttcacctgta
    17701 ttatccaatg attttgtatt tactcctaca ttttttaact tgtttataac gttcatattt
    17761 ttgtcttcct taaaaaactc gacaacgctg tttgccatta tacttccaaa ttcctctaat
    17821 tcttctagtt gctcactagt tgcagaaatt acttcatcta aatccttaaa gttatatgca
    17881 agtatcttag ctgctttaac tcctataaat ttaataccta atccatttat aagtctccat
    17941 aaatcatttt cttttgattt ttcaattgaa tttattaaat tgtcagcaga tttttcgccc
    18001 attctctcta ggtcgactac atcttccttt tttatataat ataaatcaga tacatcttga
    18061 ataatttttt catttaatag cagtgtaatt atagattctc ccaatccatc tatattcatt
    18121 gcatcccttg acacaaagtg tataattccc cttcttattt gggctgggca agacatattg
    18181 atacatttta cagcagcctc tccttctaat cttattgttg gctctgaaca aactggacac
    18241 ttttctggca ttttaaaatc tatttcatct ccagtacgtt cctcttttat aacttctaaa
    18301 acttgaggta ttatgtcccc tgctttttga actaatactg tatcatttat ttttatatct
    18361 ttttctctta tataatcttc attgtgaagt gtagctctac ttacagtagt acctgcaagt
    18421 ctgactggtt caagaattgc tgaaggtgtt attgtaccag ttctcccaac ttctactatt
    18481 atatctacta ttttagtttt cttcttttct gctgggaatt tatatgcaat tgcccatcta
    18541 ggacttttgg ctgtatatcc catttcgtct ctttgtttta tattgtttac ttttattacc
    18601 ataccatcta tttcgtagtc taaattaccc ctgttatctg tccagtattc aatatgctca
    18661 ataacttcat ctatactttt acaaacttta taactctgac ttactgaaaa tcctagtttt
    18721 tctaagtact ctagtgattc actgtgactt tctaaatcat attcttctat attttccata
    18781 ttaaatacaa atatatctaa tggtctcttt gctgttagtt ttgggtctaa ttgtctcaat
    18841 gaaccagctg caagattcct tggattagca aataatggct gttcttgttc ttcttgttgt
    18901 tcatttactt ttctaaaatt atcttttgat atatatactt ctcctctaac tattagttct
    18961 tttttatcat ttatcttaag aggtatgctt tttactgtca ttaagttttg agaaatatct
    19021 tctcctacaa ctccatctcc tctagttgct cctttttcaa actctccatt gttataagtt
    19081 agacctacag aaagaccatc tattttaaat tcaacaacat attcaacatt ttctcccaca
    19141 tattcttgaa ctctcttatc aaaatctctc aaatcttgct ctgaaaaagc atttgataaa
    19201 ctcaacatag gagttttatg tacaatttga ttgaatttat ctaatggctt acctcctacc
    19261 ctatttgttg gagaatcaat tctctttagt tctggatgtt cttcttctaa agaaattaac
    19321 tccttcatta acttatcgta ttcataatct gatatttctg ggctatcttg attataatac
    19381 ctttcattat ggtagtttat tgtctctata agtttattaa tttcatcttt tacgctcact
    19441 ttttgcacct cttcatattt ttaaaattaa attattttga aatttcccta taattaatta
    19501 tctcctattt atatattttt tagtatatca cttttttgac tttaaattta ttacaaaaaa
    19561 aactcttatt agtatcttta atacgactaa taagagtttt tatttttatc aaaatttttt
    19621 atttttaata gcctcttgat atgtctacaa cattatctac aggtttattc tccattatat
    19681 agtgtttcaa attattataa accatattaa atgttctctc tttgttttta tctgacaccc
    19741 aagaattatg aggagttact atgacatttt cgcactccca caacttattg tccttgttta
    19801 aaggttcact ttcaaatacg tctagtgcta cccctctaaa tttctctaca cattcttcta
    19861 aatctttctg attaactgta tttcctctac ctacatttat aaatacagaa ccatctttca
    19921 taagtttaaa catatctttg tttactattc catctgtact ttttgtagaa ggcattgcta
    19981 caactactat atcacattct tcaaatacaa tattcatatc ttcagtagca aaacacttat
    20041 caaagtattt tttactactt ccatcagtat tgacacccca tatttctaca ccaaaagctt
    20101 ttaatcgttt tgctgcttct gaagatatag tacctgttcc taaaaatcca actctttgtc
    20161 catacaattc tgatacagaa aaatccatct tccattcttt attttgttgt tgtttaaaaa
    20221 attttgcact atttttatat atttctaaaa tatacaatac aatactttca ccaataggta
    20281 cactatatcc gcctctatta ttagctatct taatatttct cttcaatacc tcttcttttg
    20341 gaagttggtc tatacctaca ctagttgtct gtatatattg taaatttttc atcttagaaa
    20401 tatctaatct ctcaaatgga ttgtatgtaa ctagtacatc tgctgtattt acatcctcat
    20461 tattttcaat actatcttca gagtaatgta tgacatcata gcctaattct cttattttat
    20521 caaatttttc ttttccataa tttattgtaa ataaaacttt catctcaaat ctcctctatc
    20581 tacccacatt taaaatttta tattctaaaa tataatatga tactcaatgt acattagtta
    20641 aaattactat aagatatctt aaatttactt atgttcaatt gtttttccta aagacatagc
    20701 attacgttct tgaactaaat tttctattaa attattcaat gtccaatcaa tagatgtctc
    20761 tgttaccata gctgtaaaag gtctatttat tctcactttt tttagacctt ctaaaaatgc
    20821 atttacttgt gtgcttggag tgttattaaa tattacagac ttactcttaa tcccattttc
    20881 acattcactt gatatatttc catctaaaat gtctcttatt atattctctc cgtttttttc
    20941 acttaagatt atatggtctc ttattccagc aaagttactt acgtttttaa ttaatgataa
    21001 ttctttttta ttaaagttta caagcataat acacttccta ttgtgtattt tatctaggtt
    21061 attattattt attttttcaa atgacatttt atccatcctt tcatttattt atatacttta
    21121 atttacccca tacatttaaa ataatcaatt tttatagtta atttttatat taatacccaa
    21181 attacattta aatagttagg ctatccaaaa ctaatatata aaattagaat aattagatta
    21241 atatctacaa atcataacct aaaggagaga taccatgaat acattactaa acaataaatt
    21301 tagcattaaa aacttttcaa atgcttgggt tatagtactt ggttgtatgt taatacaagc
    21361 tgttccattt agtattgtag caagtataca gcctcaattt ataagttatg ttgtagaagg
    21421 agaaggcttt agtttagctt ctttttctct aatattcact attggtacta ttgcctcagc
    21481 aattgcatca ccttttatag gtgtattatt ttctaaagtc aatattaaga tactatattt
    21541 attaggttgt ataatttcta gtattgcttt tgctactttt tctatatgta ctgaactatg
    21601 gcaattttac actgtatcat gtattgttca aattggcgct acaattatat ctgctatagg
    21661 aatacctgtt ttaataagtg cttggtttga tgaagaaagc aaaggaaaag ctttgggctt
    21721 tgcttttgct ggtggttcta ttggtagtat atttctacaa ccacttgcag ttcgcttatt
    21781 agcaactcaa ggatacagtc attcttatct aatatttgga attatagcat taatagttgg
    21841 tatacctgta atattattca tactcaggat gcctaaagat attaatgaaa aggtaaaatc
    21901 taaaggtaaa aaagagaaag catctgatac aatttctcct tcagtatggg gttatacatt
    21961 aaaagaagct aaaaaaatta aatatttttg gattatgact attggattta tatttgttgg
    22021 tatatatgtt tcatcattat ccgtacaata tccaacttat cttagaaatt atctaaatcc
    22081 tattctaata ggaacagtag gctccacatt tgctttattt gctctcattg gtaatttatt
    22141 tggaggatta ttgtttgata aattcggtca aacaaagcca cttattatat cttttgtatt
    22201 ggttcttata gcaactttat cattactatt ggtagataaa tcttctactt ttgcatttgt
    22261 atttgccatt ttgaaaggtc tttctgtatt tgcttatatg attggtcctg cttatttaac
    22321 tggctctttc tttggtcaaa aggagtttgg tagcatatat ggaatcgtac aattaatttt
    22381 tgcagtggga tttgcattag gttcaacatt atttggattg ttagttgata attttggata
    22441 cagattttct tggtctatta taattttatc agttataatt gcctatactt tacttattat
    22501 aacttctata ggaatgaaaa aattgaataa agaaagatta gataaactaa tgcaataatt
    22561 gatttttaga tatataaata ttataataaa aaagatggag cttttaatca taaataaaac
    22621 tccatctttt ttatattaca atatagctaa ttttctctta tttttaactt tactatctat
    22681 atcctcatcc ttaactatag taaatgcctt agttggacaa acatttacac atgcaggccc
    22741 ttctggcgat tctatacaaa aatcacactt atttgctgtc attctttctc taaagcaaag
    22801 ctttccttca tctacatctt ttaatgtaac tatgtctatt gcacctatag gacatgctaa
    22861 catacaagtc ttacatccta tacaattttc ttcatttatt tttattgtgt tcccaaccct
    22921 aactatacca ccatttggac atgcctttgc acaaggagca tcttcacaat gtctacactg
    22981 tattggagca cttacttctt ttgtctttat gacagaaagt tttggattaa aatttatttc
    23041 atctggattt tggtaaaaaa tatcttcatc tgaatgtgca actacacatc ctatcataca
    23101 agttctacat cctatgcatt tatcagggtc tgctataaca aaatagttca tcacttagcc
    23161 acttcctttc ctatacaata tgtagtgtgt aatagatgat gagatttttc tcctaatggt
    23221 tctaccagat attcatcata cagtttttta atatcaggat tctcatgaga ttttctaatt
    23281 gctaaatgtt catcatgcac gtatgttgct tttgttctat tttcataagc caattcctta
    23341 tattcttcta gtaaaatttt tggttgacct ccaccactta cacacccaac aggacaagtc
    23401 ataacttcaa taaaatctac atctaatttt cctttttcca agtcttcaag cactggtatt
    23461 acatttttta atccacttac tactccaact cttaagtcta agtctcctat tttaatagtt
    23521 gactttctaa aaccattttc tcctcttact tgttttactt ctacatcagg aatactttct
    23581 ccagttataa gctcatatcc tgttcttata gcagcttcca taaccccacc agtaacacca
    23641 aagatagttc cagctccact ataactacct aaaggactat caaatttttc ttctcttaaa
    23701 tttttaaaat caattcccat gtcttttatt aaataagcta gttctctagt agttaatact
    23761 acatctacat ctctatatcc actagaatcc atttcttctc tatcacattc atatttttta
    23821 catgtacatg gcattataga aacactaaat atctttgatg catctacatt atttatctct
    23881 gcaccataag ttttaaatat tgcaccaccc atctgctgag gtgatttaca agaagataaa
    23941 tgattagtta atttaggata atttttttcc atgaatttta cccaagctgg acaacaagat
    24001 gtaaacattg gaagagttcc accttctgtc actcttttaa ttaattcact accttcttcc
    24061 attatagtta aatctgctgc aaaattagta tcgtagactc tatcaaaatt taaagctcta
    24121 agtgctgctg ccatttttcc agcacctaag tatcctaaat aatatccgaa atcttctcct
    24181 aaagcaactc taactgctgg tgcacattgg accatagtga atacatttgg gtcacttaaa
    24241 gctttcttta ccttatctat atcacataca ttgtatgctg caaatatagg ttcatttata
    24301 gactctggta tctctctttc ttgttttttc tcttgtaaaa actcaaatcc ttcatctata
    24361 actgaggcat aagatttaca agtttgaaca cattgaccgc acataacaca tctatctaaa
    24421 tttatctctt gtggctttcc ctcttcacct tcaatagcat ttactggaca gacctttgaa
    24481 cattctctac atccagtaca taaatcctta tctatattta ttatcataaa cgtcacctac
    24541 ccctgtatcc tttatttatt tgttaacaat aaactaagtg cagcttgtct attcttagct
    24601 tttttgtcat ctattggagt aacaagtttt aatgcttcct gaggacatgc acttatacaa
    24661 gctatttttt cattgtcttt acataagtca catttataag ctatcttctt attttcatct
    24721 aaaattactt gttctacttc tttaccatct tcatattgtg gaagtaagtc tatagctcca
    24781 aaaggacatg ctagcagaca agttttacat cctatacact tttcttcatt tacagacata
    24841 atattgtttt ctttgactat agctttttgt ggacatgagt ttaaacaagg agcatcttca
    24901 caatgtctac actgtattgg catacagaaa ttgtcacctt ttactaaata aagtctaggt
    24961 ataacaggta tatcaactgt tccaactgta taacccacat tattatgctg attatgcatt
    25021 gtaaaacaag ctacttcaca agctctacaa cctgtacatt tgtttgggtc cgctattaca
    25081 aaagagccta ttttattttt catattatcc cacctttcaa tttgtatatt tccaaccctc
    25141 tagttacttt catcatatgt ctacttactt tcatcatata tatttatata tttcactatt
    25201 ttctcagtac ttatatttcg tttacatttt ttgcacaaat tgtatgtgtt attatacacc
    25261 ttatcagtat cgtcaattaa tactccacag atttcacact ttgaacattt ttgttcttct
    25321 tttttagtac acatatactt attctcactt tttgataact ctctgtggat ttgcataaat
    25381 attcatccgt cttcctctta caaatccaat aagcgttata tttaattttt tagaaagctc
    25441 tattgataaa ttagtcggtg ctgattttga tactacaata ggaatttgag tcatagctgc
    25501 ttttaatatc atttccaatg atattcttcc acttacagcc acaattttat cttttaatgt
    25561 tatgtcattt aaaacacaaa atccaatagc cttatccatt gcattatgcc ttgctacatc
    25621 ttcacaagtg actatttttt tatcaaaatt aaatacagat acgctatgta ccccaccagt
    25681 atttttaaat aattctgatg aatttaaatt tctatccatt atactgtaaa tcgtatcata
    25741 atttattttt atattgctct ttacagaagc acatgttata tagtctagtg aatttaaaaa
    25801 tataatttgc tcatcttgat ttttttgttt ctcttgaata gttacttttg caaaacgttc
    25861 actctcattt atctcaaatt ttaataagct ttttttgttt tctatgtatc cttttgtttt
    25921 caagaatcct actataagtt cctctaaatt atatggggta cataaaaatg tatttacata
    25981 tttatcattt aaaataaaac taagtggata ttctgcaatt atttcttcat tatctattga
    26041 tacttcatgt tcatttattt tgcttacttc tacattaaaa gaatttaaat atttgtagtc
    26101 cactagacta taactatttt caaaaacttt atccttacta ttttcttgta atttagtttt
    26161 tgagtatata ttgttcataa aatattagcc tttctgcctt ttataaatct taaatctaat
    26221 aaatcattag atatttgtag ctgtcatttt gtctttcaaa ctttgatact cttctcttac
    26281 acatttttct gctaattctt ggtcttcaag tttttcaagt ttaacagcac aatatttata
    26341 ctctggtgtt ttagatatag ggtctaaatt tgctattgta agttcattgc aagctccaac
    26401 ccaccactga taagtcatat atgttgcacc ttccttaact ctatcagtaa ctgttgctct
    26461 tgttattaca cttcctcttc ttgaactaat tctaactaat tcatcatctt caattcctaa
    26521 tttttcagca tcattagaat ttatttgaac acgtcctggt tcatcttcta atgaactaag
    26581 tgttctacaa ttaccagtca ttgttcttac tgaatagtgt ccaacttctc ttacagtaca
    26641 taaactaaat gggtattcat catcttcaac ttccataggg ggtctccatt cagcagcgaa
    26701 caaattacct ttaccattag gagtagaaaa cttttgacct tcatacagat acatagttcc
    26761 tttatcttcc atactttcac tcttacaagg ccattgaacg catccttgag tttctatctt
    26821 ttcataagta gcacctaaga aacttggaca taattgtcta agttcgtccc atatttcctt
    26881 agtattttta taattcatag gatatcccat tgctgtagaa atttcgctta taatttgcca
    26941 gtctggtttt acatctcctt gtggctctat agcttttctc atcaattgga atcctctatc
    27001 tgcacatgta tatactccct catgttctcc ccaagaagta gctggaagta taacatcagc
    27061 gtgaagtgct gtcttattca taaatatatc ttgaacaatg acaaattcca attcatctaa
    27121 tgcttctcta acttctgatg catctgggtc actttgcact gggtcttctc caaatatata
    27181 ataagcttta agctttttct cttttagtac taagtctgga acttgagtta aactataacc
    27241 attattagga gataatttta ccccccaagt tttttcaaac ttttctctta tcttatcatc
    27301 agttacattt tgatagccag gatatacatt aggaagagct cccatatcac aagctccttg
    27361 aacattattt tgtccacgta caggtcctat tccaacgctt tctctaccaa aattacctgt
    27421 aagtagagca attgatgcaa gtcccttaac tacatcaaca gcttgaccaa attgacagac
    27481 acccattcca taaagtatca tagctttttt cccttttgca tattctctca tagcttttct
    27541 tataagctct tctgggattc ctgtaatctt ttcagcatat ttagctgtat atggtttaac
    27601 aatttcttta tactcatcaa atccttgtgt atgattttga acaaattctt tattgtaaag
    27661 accttcttct attaatacat ttccaaaagc atttactaaa accatgtttg ttccaccttt
    27721 tataggtaga tgtatatcag caattcttgc agattcagtt actctagggt cagtaacaat
    27781 taatttagca ccattttttt tagcttttac tattctattt gccactattg gatgtgaatc
    27841 tgctccatta tatccaaata taaataaaac atctgcattt tctatctctg gtatagaatt
    27901 agacattgca ccactaccta atgagtaagc cagaccggct acagatggtg catgtcatac
    27961 acgagcacaa tggtcaacat tattagttcc tattgtagct ctcataaatt tttgcattat
    28021 atagtttgct tcattaccag gtcctcttgc agaacctgtt cccataatag aatcaggtcc
    28081 atatttttct ttaatttcat ttagtcttga agctgtatag tttatagcct catcccattc
    28141 aacctcttct agtacacctt gttttcttat cattggcttt tttagcctag gtgttagaat
    28201 tttagggtca tttagaaaat cccatccata acgacctttt aaacacaaac tttcttgatt
    28261 tgttctacca ttagccggtt cagctcttaa tatcttatta tcttttacta ctagatataa
    28321 ttggcatcca gctccacagt atggacaaac tgttaaaatt tttttctcca tacaattccc
    28381 cccttagatt tatcccctga taagtaaaat ttatcttaaa gataatgagt gtttttataa
    28441 tatgcaaaat cataaatatt ttgcaatact atctattaaa ttcaacaatt ttctcacaat
    28501 tcctcttaaa tttcaaaaaa aatatgtcat aataatcttt atatttcaac catttaatta
    28561 aaatattcta gttcttatga catatttttt aatatactca atatatattc tttaaactta
    28621 tttagttttc acttaaaaca atatttctgc caattctatt tttcattcta tagtgagtaa
    28681 ttacagtaaa tattccactt ccaatcagaa ttaaatagta cataaatcca ccatataaaa
    28741 atactgcata tcccatcaaa ggttgaggga atactgattt gaatatagtg aaaaaggcta
    28801 attcattagc tcctacattc ccaggtgttg gtattggaga tattgccata tataaaaatg
    28861 cttgtaatgt cagtatataa atatagctat aaccttgtaa attaaatgct ttatatatcc
    28921 aaaatgaaac actaaagtaa gcacttaatt gaataaaagt tactattata gttgaaatta
    28981 ataccttttt attctttgca
//
LOCUS       DACTGW010000029        26713 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.029, whole genome shotgun sequence.
ACCESSION   DACTGW010000029 DACTGW010000000
VERSION     DACTGW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.029"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     regulatory      complement(558..654)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1450..1851
                     /locus_tag="I9573_000977"
     CDS             1450..1851
                     /locus_tag="I9573_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HAT4795606.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            1848..2915
                     /gene="rsgA"
                     /locus_tag="I9573_000978"
     CDS             1848..2915
                     /gene="rsgA"
                     /locus_tag="I9573_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAT4795607.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKSGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            3451..4149
                     /locus_tag="I9573_000979"
     CDS             3451..4149
                     /locus_tag="I9573_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795608.1"
                     /translation="MKISSQYRSQYSFRHESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            4251..4799
                     /locus_tag="I9573_000980"
     CDS             4251..4799
                     /locus_tag="I9573_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795609.1"
                     /translation="MEGLGFYAAALSGSSYQRIGFGKLDPIEVIADGDWISYKQAQDT
                     LTVIRNFLNSFDWRNASEMERANRAAKLVTEAKYVDSKYCNIVYGNLVDKRGVCGSFA
                     SSFHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRSDGSEISAFGGALDFDYRKLK
                     DATREMTTYYDAKALSILGFNQ"
     gene            complement(5200..6156)
                     /locus_tag="I9573_000981"
     CDS             complement(5200..6156)
                     /locus_tag="I9573_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT4795610.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDNNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSYGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIQFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENKSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            6859..7854
                     /locus_tag="I9573_000982"
     CDS             6859..7854
                     /locus_tag="I9573_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT4795611.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVLKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            7841..8695
                     /locus_tag="I9573_000983"
     CDS             7841..8695
                     /locus_tag="I9573_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4795612.1"
                     /translation="MSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFDVVV
                     NMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSEEIC
                     NRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFTSNP
                     VQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPLVLL
                     PETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYKRMR
                     TLFDGVGI"
     gene            8695..9486
                     /locus_tag="I9573_000984"
     CDS             8695..9486
                     /locus_tag="I9573_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4795613.1"
                     /translation="MKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLMF
                     NSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYITK
                     PLDPLFRLTFENINIGSSPLILASILIVLYGISKLNSEITLVMIIIYIFMVLLMTLLF
                     YDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMST
                     IPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            9771..11150
                     /locus_tag="I9573_000985"
     CDS             9771..11150
                     /locus_tag="I9573_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT4795614.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKELLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            11488..12318
                     /locus_tag="I9573_000986"
     CDS             11488..12318
                     /locus_tag="I9573_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4795615.1"
                     /translation="MSIRDVTISNVKARVVNIKKQDIDTARLEYNKFLWRDFNMDTYF
                     NKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAISLCKIACPDVVILDIMLPD
                     IDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGDDYITKPFSPREIAFRVKAQ
                     LRRQQYQSIVPSDSAVIKIGDITIDIEGNRVYKDRNEIELTGREYHLLSYMAQNVNKI
                     IGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSNPKILITVKGLGYKLVNRID
                     "
     gene            complement(12590..14128)
                     /locus_tag="I9573_000987"
     CDS             complement(12590..14128)
                     /locus_tag="I9573_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAT4795616.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVEEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            15011..15466
                     /locus_tag="I9573_000988"
     CDS             15011..15466
                     /locus_tag="I9573_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAT4795617.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            15515..16099
                     /locus_tag="I9573_000989"
     CDS             15515..16099
                     /locus_tag="I9573_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HAT4795618.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSREQMDEVKLAMGFKL"
     gene            complement(16104..16427)
                     /locus_tag="I9573_000990"
     CDS             complement(16104..16427)
                     /locus_tag="I9573_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4795619.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLESL"
     gene            16579..17097
                     /locus_tag="I9573_000991"
     CDS             16579..17097
                     /locus_tag="I9573_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HAT4795620.1"
                     /translation="MNFVELTKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGRQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(17273..17941)
                     /locus_tag="I9573_000992"
     CDS             complement(17273..17941)
                     /locus_tag="I9573_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795621.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            18056..19471
                     /locus_tag="I9573_000993"
     CDS             18056..19471
                     /locus_tag="I9573_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAT4795622.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNSKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQYVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            19670..20152
                     /locus_tag="I9573_000994"
     CDS             19670..20152
                     /locus_tag="I9573_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4795623.1"
                     /translation="MKTNTDVIMEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFT
                     NVVGCTIHQYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQ
                     VYRNKDEFYPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA
                     "
     gene            20149..20733
                     /gene="clpP"
                     /locus_tag="I9573_000995"
     CDS             20149..20733
                     /gene="clpP"
                     /locus_tag="I9573_000995"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAT4795624.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            21055..22131
                     /locus_tag="I9573_000996"
     CDS             21055..22131
                     /locus_tag="I9573_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT4795625.1"
                     /translation="MIAISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            22198..24237
                     /gene="bclA3"
                     /locus_tag="I9573_000997"
     CDS             22198..24237
                     /gene="bclA3"
                     /locus_tag="I9573_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HAT4795626.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGGTGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGGTGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGATGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGVTGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGPTGPTGA
                     SAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAF
                     SMPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPL
                     TGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            24406..25167
                     /locus_tag="I9573_000998"
     CDS             24406..25167
                     /locus_tag="I9573_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HAT4795627.1"
                     /translation="MKNKIETSMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            25476..25754
                     /locus_tag="I9573_000999"
     CDS             25476..25754
                     /locus_tag="I9573_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795628.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGEYIYIPRKSNNKKEWGSSTS
                     TREDLSIRNMQIYEDYQVGYDLKYLSEKYYLSLKSIQRIVLQEKRKDS"
     gene            26047..26622
                     /locus_tag="I9573_001000"
     CDS             26047..26622
                     /locus_tag="I9573_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HAT4795629.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPETLEQFHTNDYNLAKQYAMNTELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
BASE COUNT         9912 a         3211 c         4473 g         9117 t
ORIGIN      
        1 agttataaaa gaataagtta tataaaaaat tattatattt tttaatatag cttatttgca
       61 aatttataat tttgttaatc taatccttca aactaaaaaa tcatttatgt ttacagaatt
      121 tcattttaag agatgtttat tttcacatag ataagagaat ttaagttctc atatttatta
      181 tttgatacaa atttacatct ttattatatg tattttaaat agaaaataaa attatgaata
      241 ctagaaatct atcttaatta gctttctaac ataaggctat ttaaagtaga taaaactaaa
      301 aagggttctc tagaaaacca aatctattta attatactta cttactaata ccatcacttg
      361 atgcaaacaa gattctacca cattttctcc gtactctagt ataaaatcat ctagttgtgt
      421 atctataaaa tcacaataat catatgaact accaatataa gcagtcaaca tatgtgtaca
      481 tctatccatt ttatctctaa acatatatat tattatcccc ctaaaaaact ttcataaaaa
      541 caaaaagaat atctcttttt ggcaactggc tgacataact catagtttat tttataagcg
      601 ttagtcccta tagctttgcg tcactaaatt tctctagttt tgccgattta gttttattct
      661 acaattacaa taatacaata gaattgctta ttattcaata tacttatttg aaaattaaat
      721 aaatttacaa atatgaacgt tattttatat aaaaaaaata gactattcat gtagaatata
      781 tggaaaaact tgattgtagg ttttgtatga aaagacagtt gttattaatt taagtgagat
      841 aaattgagca ataggcataa aatgagaaaa caaataatag caattaattt ttattattaa
      901 tactaggtga acattaaatt ttaaaatacc actagatttt atagaataag aaaatgagac
      961 aaacataaat atatgaatat attaaattat acttgaacat acttatattt tatgataaaa
     1021 ttttaataat aattattagt gaaggagtgt cttgcctgat gtcagagtta tatataatgg
     1081 taaccaacaa ataaatagtt ggagacgcat atgggttctg tttaatcaga atttttaagt
     1141 gtatttttga tacatttgat tgttgtgtct tactctgcct ttatagataa ctaacattag
     1201 acaacaaata ataatattac atattacttt gttaaattgt ggtgtagtgg gctttaatct
     1261 gctacacttt tattaatttt acacagtatt attttgaata taattatgtt gaaatagaag
     1321 atactaaatt ttctagtcca caggggtttt ctgtttttgt gtaggtaaat atgtatacca
     1381 cataaaaagg cagatataaa tacctgtgga ctttttttgt agaaaataca agatacggag
     1441 gaaataaaaa tgaattataa aaatagaaaa cgagcttatt atcaggaaaa ttcctgtatg
     1501 gataacttta tttgcaaaaa ttgtggttgc cttgtagttc ctgaaggagc tggtacacaa
     1561 catagaaatc attgtccaca ttgtttacat agtcttcatg tagatatcat tcctggtgac
     1621 cgtgctgcag attgtgatgc taatatggaa cctattggag tgtgggtgag aaaaaatggt
     1681 gagtgggcaa ttattcatcg ttgtacacgc tgtggacatc ttagttctaa tcgtattgca
     1741 gctgatgata attcagtgaa attaatgtca attgcattaa aaccactggc acagccccct
     1801 ttcccactag aaagaatcat tgaaaagttg gaaaaggagg aaacagaatg aatggtatga
     1861 aacaaatcaa tttacaagac tatggtctaa ataggaattt tattactcaa agtatggaat
     1921 atcacgattt ataccttgct cgaattatct cgcaggaaaa gaacttttat caagctgtat
     1981 gtgtaaatgg tgtactgttt gcagaggttt caggaaaact gcgttttgag gctactatac
     2041 aatctgattt tcccacagtt ggtgatttcg ttatgttgga tcgttgcaat aataaaaatg
     2101 gtaatgctat tattcatcat gtgcttcata gaaaaagctt gtttgtgcgt aaggctgctg
     2161 gaaaggtaaa tgaggaacag gtggttgcag ctaatattga cattgttttt ttgtgcatgt
     2221 cactgaataa ggattttaac attcatagat tggagaggta tctgtcttta acatgggaaa
     2281 gtggagctat cccagtagta gtacttacta aatcagactt atgtgacaat gtgcatcgta
     2341 aattacaagt agttaattct atagccttgg gtgtagatgt agtagttacc tcatctatag
     2401 agccagatgg ttataaacag atttctaagt atatcaaatc aggctgtact gttgcattac
     2461 ttggctcttc aggagttgga aaatcttcat taattaattg tctgctagga ggagaatttt
     2521 taaatacaaa ggaaattcgc atagatgatg ataaaggaag acatgccacg actagacgag
     2581 agctattttt aatttcaggt ggtggtattg ttattgacac accaggtatg agagagttgg
     2641 gaatgtggga tgtttcagaa ggtttagata aaagttttgc agatatagaa gcttatttcg
     2701 gaaaatgtca ttttcgtaat tgtactcaca cctcagaacc aggctgtgct atctgggaag
     2761 ctattgctca tcaagaactt tcgcctaaaa gatgggaatc ctatcgtagg cttaaaactg
     2821 aagcagtatt tgcagacaat aaaagcgaat acttagcaaa aaaagaaaag aaatttaaaa
     2881 atatctcaaa aataaataag acgattagaa gataagtgta gtttttttgt tcattctatg
     2941 agtctatata ctattaatgt attaaatttg ccattaattt gttactttaa tgtgattttg
     3001 atggcaaatt tggtttgtgg tattatttaa agcaatttat taattttttt aataaagttt
     3061 gattttttat atgatgatat atatatgtga taatgtatac atatttgtta cgatagtctc
     3121 ctttataaag aggtcttatg taagataaga aaaggcatat ttatactaat acataataga
     3181 gtatatgctt atgtatgata gagatgtttc ttaagagcaa aactgcaata ttgctaattt
     3241 ataaagtaaa aatatatgaa ttataaaata caaataagat atttataaaa attattgttt
     3301 taatttataa taaaaatttt ttgttgaaaa caatgctcga acctataaaa ctattaacta
     3361 atatttgtaa atgtatactt ttgatgataa aatataatta agttaagttg gtttttataa
     3421 acataaattt attttagaga ggtgaaataa atgaaaatat ctagtcaata tagaagtcaa
     3481 tattcattta gacatgaaag taatataaat aatacaagaa taaatgaaag tatggttaag
     3541 aaaaatgaaa ctgtaggaaa agacacttat ttatctaata ttatgaaaca aaagcaagaa
     3601 cttaatgata gaattagaga tttaaaatat agacaagagg tttatactaa aaaaataaat
     3661 gaagcaatta agaacttata taaatcagaa ataagagaaa caactaataa cttttctaat
     3721 atagaaatag gtattaaaaa tagcattata gaagataaaa ataaaagtac aatgttagat
     3781 gaaaattcaa cttatctaaa tacaaatgat gaaaaagaat ctttaatcac taaagagtct
     3841 aatgaaaaaa ttgaagaaga aatattaaat gatgaaaaat tagaagagtt agaacaaaaa
     3901 aaggattata aagaggattc taataaaaaa gagaaagtat ctgaggactt atctttagta
     3961 ggtaaaactc gtgaagagct tgaaaatatg cttaaaaatt ttataaattt aacacaagaa
     4021 gaaataatga aacttgagtc gagaatagaa aagttagata aagatgctga agaatacaaa
     4081 caaaattcaa agactaatat atttgataaa acagatgaac aaaaaaaaca tataaatgta
     4141 ctgatttaaa caatatgttt taaaaaaata agtttaattt actctatgga ggaaatccaa
     4201 atggagttga gcttcgagca gatggtaaat attattatat aaataagggt atggaagggc
     4261 taggatttta tgcagcagca ttgtcaggta gctcatatca gagaataggc tttggaaaac
     4321 ttgaccctat agaagtaata gccgatggag attggatttc ttacaaacaa gctcaagata
     4381 cacttacagt aatcaggaat tttttaaata gttttgactg gagaaatgca tctgagatgg
     4441 aaagagctaa tagagctgca aaattggtaa ccgaagcaaa atatgtagac tctaaatatt
     4501 gtaacatagt ctatggtaat ctggttgata aacgtggtgt atgtggaagt tttgcaagta
     4561 gctttcattt attaacaagg ttaatgggaa tggattcact atctatatta aatccatcat
     4621 taaatcatgc ttggaactat atacaaattg atgggaaatg gtataggtct gatgggtctg
     4681 aaatatcagc ttttggtgga gcattagact ttgattaccg taaattaaag gatgcaacaa
     4741 gagagatgac tacttactat gatgctaagg cattgtctat attaggattt aatcaataaa
     4801 aataggggag gctttataaa gagccttttt ttattgattt gaaatttaat aaggaatttt
     4861 atatgaaaat ttaatcaata aaagcaaaaa aggctcttta taaagccttt ctaagtgttt
     4921 ttttaagtaa aattttttat atcaacatca gttttaatca aaaaatataa cattttggac
     4981 ttacttttaa aaataaaatt attaaaatat aatattaatc atgttaataa tgtttttagt
     5041 ttattatatt ctataatagt ggtcatgttg actattgtag ttttattaag aatagttggt
     5101 ttatataaat atatgcaaag atagcttgtt gtttacttta cttattagta tataggacaa
     5161 gatactctaa taaatttgcc tattttaata tcttttgtac taaaaccaac taaagtttcc
     5221 tttattattt cgttttgaat aatacaaagc ataatccgat tgtttgtata aagactggaa
     5281 agtatcagat ttattttcta atttagatat acctatagaa caagaattac tctctagaca
     5341 taattctaaa actttctttg ctttattttc aacattctct tttgattgat tactatattc
     5401 aaaaacagca aattcatctc caccaagtct tccaataata gcatcattta cgaatacttt
     5461 agtcaaaata tttgaaaact gtattagtat ttcatctcct cgaacatgac caaagatatc
     5521 atttacctgt ttaaaattat ctaaatcaaa gatgtaaaag tatccatatg aaacagtttt
     5581 aagcatttct tttatgtgta cttcaaaaaa atgtctattg tatagtccag tcataaggtc
     5641 tttttctgat tttagtttaa attcatttgc cattgctagt gcttcttgtg ctttttcagt
     5701 tattgtttta gggtaatact catctttcat ttggtcaaag tttggaatag aatggtgaac
     5761 atggataagt ttaatcatat catctgtaac tctgtatacc atagtaaatc gagtatccat
     5821 ttctacatag atatcttttt ctaggtaatc tttttctatt acccaaagag taccatatac
     5881 tataaaagct tcgtctgtaa ctttggtaca tgaataccac tcatctataa tctcaaagtc
     5941 tatttgctct gattctgttt ggtctttttc aagacctata ataaattctt ctaatgtttc
     6001 gtaaaattca tgttttcctg ttccaattaa tgcaaggttg ttatctaata aggaaataat
     6061 aaatttaagg ttttcttctt ctggatttaa aatataaata tgccaaattt tttttgataa
     6121 atctcttaat tgtttttgta ttgtattatt ttccatagat taccccctta agatttacag
     6181 tttatatttt actgtattat aagttgaact cttattacaa tattattata atatagaact
     6241 aaagaaatta catcctgtat aggtaaaagt atatctttta caataaaaat attacagtaa
     6301 aataaccagt tttaatatac gtattatatt tttaatttat aaacgaatat tataaatgaa
     6361 atttaaattc aaccctgaag aaataagcta aatgtattag tatataaatc catacttgaa
     6421 cataaataac ctttatgata tgatatcaat aataatatta tgagaggagt gccttgatgt
     6481 cggagttata taatggtaac caacaattaa atagttggag acacatatat tctgtataat
     6541 cagaaatttt aagtgtattt ttaatacatt taatttttgt gtcttattct gcctttatag
     6601 ataactgaca tatgacaaca aatagtattt aaaatatata ttactttgtt aaattatggt
     6661 gtagtaggct ttaatctgct acacttttat taattttgta tagtattatt ttgataacta
     6721 ttttgaaata gagagcatta atttttctag tccacaggag ttttctgttt ttgtgctgtt
     6781 aaatataagc acagaaaaag gcagatatat acctgtggac tttttttata taaattatga
     6841 aatatggagg tagttaaaat gagtataatt gaacttaatg aactgactaa atcttataga
     6901 agatttgaga aaaaggcagg atttatgggg agtgtaaagt ctttatttaa taggaaatat
     6961 acagaaaaaa cagctatatc gtcatttgat ttaaagatag aagaaggaga atttgttgga
     7021 ttaataggtc aaaatggagc tggcaaaact actcttatta agatgttaac tggtattatt
     7081 gcaccgtctt ctggagaaat atctgttatg ggttattacc ctaacaaatt agaaaataat
     7141 tttaaaaaac aatatgctgt tgttatgggc caaaaaagtc aactattctt tgaattgaca
     7201 ccagcagata cgtttctttt atttaaagaa ttatatgata tatctaatga agaatataaa
     7261 aaaaacttgg attactttat cgaattattt gaggtatctg actatttaaa tgttcaagtt
     7321 aggacgcttt ctttaggaga aagaatgaaa atggaattaa tagtagccct tctacataat
     7381 ccaaaaatat tatttttgga tgaaccaact ataggcttag atgctatagc ccaaaaacaa
     7441 attaggtatt tcctaaaaga agtaaatgaa acaaaaggaa ctactattat acttacttct
     7501 cattacatgg aagatattaa gagtctctgt agtagaagta ttgttgtaga aaatagccgt
     7561 aaaatatatg atggttctac agagaaacta tttaatcatt ttcaaaaata taaaaaaata
     7621 acagttagtt tttccaatga aactaacatt gtatttccaa aagattgtaa agttgttgaa
     7681 caaacaaatc ataagacatc agtaattgtg ctaaaagaaa gaggaaatga aacgattaaa
     7741 tggatttttg ataaatatga tgttaaagat gtaatgattg aagaagaaga tataggtagt
     7801 attgtagaga gaatatataa gaaaggaagt gatgagtatt atgagcaact ataatgaagc
     7861 aagttcaaat tattcaatgt tttataaatt taaacaaagt attagaaggt attatgaagt
     7921 agccaaaatt aactttaaag tgcaacttat ctggagattt gatgtggtgg ttaatatggt
     7981 gtttacgatt gtaaaaattt tatttgctta tattttatgg aaagcaattt ttggtgaaaa
     8041 taaaatagtc tctggtttta catttaactc tatgttgtct tactatataa taagctcatt
     8101 tttagcaggt attgatatgt caaagagtac cagtgaagag atatgtaatc gtgtaaaatt
     8161 gggcacattt tcaaagtaca tgattattcc aattaatgtt caaaattatt ttattgctaa
     8221 aaattttgga atgacatgtt tttattttgt atttaatatg atttctgcat ttgtatggat
     8281 ttttatattt aaaatagatt ttgcctttac atcaaatcca gttcaaatat taatttcaat
     8341 tttgatgatt tttctagggt ttatttttat gatgaaactc cacttcttta ttgggatttt
     8401 gtcatttaaa tttcttgaga ttagtttttt tagaatgata attgaaaata ttgttgaatt
     8461 tattacagga acaatgatac cacttgtact cttaccagaa actatagtaa cagttatgaa
     8521 ggtatttcca ttttattata caacatattt gccatctatg ttactaatag gtagaaacag
     8581 caatgaggca atacaaggat taattattct agcattatgg gttttatttt tttctgtagt
     8641 aaataaagtt atgtataaaa gaatgagaac attatttgat ggggtgggta tatgatgaaa
     8701 aaaaatttga aaataatatt tatgttatta aaaatgaaat tatcaaagat gatggtattt
     8761 agatttgatt tttttggagc attttttgta gatagttcat tatttatatt acaacttcta
     8821 atgtttaata gtatatattc tcatgtagac tcaatagggg gatggcaaca aggagagatg
     8881 ttaatattta taggaacttt ctcattaata aatgcgataa atatgacgat tttctttttt
     8941 ggtatttatg atattccacg aaaaatccaa gaaggagaat tagactacta tattacaaaa
     9001 ccattagacc cactatttag gttaacattt gaaaatatta atattggttc atcaccatta
     9061 attctagcaa gtatacttat tgttttatat ggaatatcta agttaaactc agagataact
     9121 ttagttatga taataatata tattttcatg gtgttattga tgacattact tttttatgat
     9181 gtatgtatca tacttagaac agtttcattt tttgttatat catcttctgc gataatgaga
     9241 ttagaagata atttattgcc aatgaatatg aaaattcctg gtgtaatata tgaaggtgtt
     9301 tttaaagtat tattctattt aatcttacca tatggaatta tgtcgacaat tccgactcaa
     9361 attttggctg ggacaataac aattaatgga ttaatatact caattttaat tgtatttttg
     9421 tttacattgt ttatgtggag attttggaag ttaggtatga gacattataa aagtgcaagt
     9481 agttaataaa ttagtaaaaa tagacaatgt ataactatat ataaatacgt ttcatatgac
     9541 actaaactat gtgaacaagt atttgcgcta aaggcactga aagctataaa atgattaaag
     9601 taaggattta aaagtttctt tagtagaaat ataattgtgt aaaaaagcaa gattaattaa
     9661 aactatatcc aaataattag atataattct tatgcagaaa ggaggtctgt aaatgtctaa
     9721 ttgtttggaa tatattttaa aaataattaa tgtacagaaa aataaacaaa atgagtgatg
     9781 tatattattc agaaaatcat gaacaagata atctttatga gcaagataat ttactggcac
     9841 aaagatttaa tttatttact ctattgaagt ttgcatttcc tactattttt atgatgattt
     9901 ttatggggtt gtatacaatc gtagatacta tatttgtatc aagatttatt aatacaaatg
     9961 ctctatcagc aattaatatt gtatgtccaa ttattaatct tatagttgga attggaacta
    10021 tgcttgccac tggaggaagt gctattgtag cacgtaagat gggaaatggt gatattgagg
    10081 gagcaaataa agctttttct ttaatcgtat tatcaggagt tgtaattggg gtaataatta
    10141 ctggtatagg tattgtattt atggattata tcattatatg gttaggagca agtgaagttt
    10201 taattaaata ttgtcgttca tatcttttta ttatcttggt ttttgcttct gcaaatatac
    10261 tacaagtatt atttcaatgt ttatttgtaa cagctggaaa gcctcaattt ggattatggc
    10321 tagtgttagg tgctgggtgt gctaatataa ttcttgacta tgtgttcatt gttctgttac
    10381 aaatgggtat tagtggagca gcacttgcaa ctggagtagg atacctaatt cctaccgttt
    10441 caggtatggt tttcttttca aaaaataaag agttacttaa gttttgtata cccagactag
    10501 ataaaagaat attatttgaa gcttgtttta atggttcatc tgaaatggta ggtcaactat
    10561 caacagcagt tactactttt ttatttaatg ctatgatgat gaaattaatt ggtgaggatg
    10621 gagttgcagc tattacaata attatttatt ctcaatttct tttaacttcg ctttatatag
    10681 gtttctcaat gggtgttgca ccaataataa gttataacta tggaagtgaa aataatacac
    10741 agttaaatca tgtttttaag atttgtatat tatttatttc agtggcatct ttattggtat
    10801 ttatcttttc tatatcatgt tcaccaatca ttgtagagat tttttctcaa agaggaagcc
    10861 gagtttatga aattgcaagg tctggatttt atatattttc ttttagtttt attttttgtg
    10921 ggtataatat ttttacttcg tctatgttta cagcattgtc aaatggcaag gtctctgcaa
    10981 tattatcttt tctacgtaca tttggattta taactatagg tttattgata ctgccaaata
    11041 tattaaatat aaatgggata tggttagcag taccaagtgc tgagatttta actttatttt
    11101 taaccatttt ttgtatatgt aagtataaga atagatataa atatatgtag atattaaata
    11161 aatagtttag gtagatattt tatgttttaa taagtaattc aattagtggt agatatatac
    11221 tattgaaaat taaagttata attgatataa agaattaata aaaataacat ataaaagttt
    11281 aaaagggagt tgaacttaaa gtttgattag aaaaagtcaa ttttaagaca actcctttta
    11341 ttttactatt gtattaatct gtatctgtat ctgtaagtgt attagtctaa tattataaac
    11401 tttggatgaa attaattttc tataggtttt aatgtatcat acactttatt ttaaagaaac
    11461 ttatcttaat tttcatagtg attgaatttg tctattagag atgttactat tagtaatgta
    11521 aaagcaagag tagtgaatat aaaaaaacaa gatattgata ctgcaagatt agaatataat
    11581 aaatttttat ggagagattt caatatggat acatatttta ataaaaaaat attacttgtt
    11641 gatgatgaaa aagatatagt agatttaatt gaagaagtac tcataaatga tggttttaaa
    11701 aacattatta aagcttataa tggtttagat gctatttcgc tgtgtaagat agcatgtcca
    11761 gatgttgtca ttcttgatat aatgttacca gatatagatg gtatagaagt gtgtaaaaaa
    11821 attcgagagt tttcatactg ttctattctt tttttatcat caaaaaatga tgatattgac
    11881 aaaatactag gtcttagtag tgggggagat gactatataa caaagccatt cagtccaaga
    11941 gaaattgctt ttcgagttaa agcgcaatta cgtagacaac agtatcaaag cattgtacca
    12001 tcagatagtg cagttattaa aataggagat attactattg atatagaagg aaaccgtgta
    12061 tataaagata gaaatgagat tgagttaact ggaagggagt atcatctttt aagctacatg
    12121 gctcaaaatg taaataaaat tataggaaaa gaaagactct atgaacaagt atggggagta
    12181 tatagtagta tttgtgacaa tacaattatg gtgcatatac gacatataag agaaaaaatt
    12241 gaagataacc cctcaaatcc taaaatactt ataacggtta aaggactagg atataaactt
    12301 gtgaatagaa tcgattagaa tagattttaa tattaatgaa gatgtctcaa gttgaatttt
    12361 tagttaatga ggcattcttt tatgtgtgaa gtcaaatgta ttttcgttat ctcaacaatg
    12421 aagaagaggt ctatctctat tttgagatag cctcattaag tttatatcct ggtaatgtac
    12481 tttatgttct aataaaattt ttttagttta tttgcagtga aacattaact agaacattaa
    12541 aatttgcatt gaaatatcaa ttaaataata ttgtatattg tgtataaact tatactaatt
    12601 gtttttctat attaaacact ttatcaatcc aacctttata aaatttttct tcatgggaaa
    12661 ctaggattat acttcctcta aactgtttca aagcattttg taaagcatcc tttgtctctg
    12721 catcaaagtg attggttggt tcatccagaa taagaaaatt acaaggagat aacatcatac
    12781 aacataattt aaccttagat tgttcaccac cacttaaagt agatacactc cttgaaacat
    12841 gctcttcctt cacaccacat ctggctagat gacgtctaat ctcttttgta tttaattttg
    12901 gatatttatc tgctactatt tgaagtgggg ttttatcagg attttcccat tttaaatctt
    12961 gctcataata accaatttta acttgttctg aaaactggaa atctccagat atacgaggaa
    13021 tatctttgac taatgtcttt aacaaggtag atttaccgat tccattaaaa ccagttataa
    13081 ctattttttg acctccatca acagaaaaat ttaattttgg aagtaaagaa tagtagtatc
    13141 caacttcaag atttgttata gtaagagcat tttgagccga tatttcaatt tctgaaaatt
    13201 gaatatttgg ttttcctgta aaactaggag gagctattct ttctatttta tccaattgtt
    13261 tttgtcttcc tcttgcaatt tttgaattta ctccagcttt atttttacgt ataaatgtct
    13321 cttctttttc tattttcttt tgttgtgctt gataccttct aatataatct tctcttaaat
    13381 gtgatttttg tttaaggaac tctgaatatt ttccatgata ttttttaatc ataccaaatt
    13441 ctatatcaag aatacctgta gaaatttttt ctaaaaaatc aaaatcgtgg gaaactacaa
    13501 taaatgcccc attaaatgta tttaaataat ttgagagcca ttcaacatgt tctttatcta
    13561 aaaagtttgt tggttcatct aataatagaa catcatggtt agaaagtagc aattttgcta
    13621 aaattacctt tgccctttgc ccaccactaa gctcttcaac aacacgattc attcctattg
    13681 aatctaatcc aagaccattt gctattttat ttatttcatt atctatagaa tagaaattac
    13741 ttgctattag ttgttcctga taatctgatg ctttgattag atattgctca ttttcagata
    13801 tagcactttt ttgatagagt aaattcattt ccttttctat tttataaagt tcttcaaatg
    13861 cagtgtgtaa atagagtgat attgttgtat ctctattgat ttctgcatat tggtctaaat
    13921 ggcctatatt gatattaggt tgccacttga tttctcctga atctggaaca acttctccca
    13981 ataaaatttt aattaatgta cttttacctg taccattttg gccaactaca cctatatgtt
    14041 cacctttgaa taaatcaaag ttagctttct catacagtga tttatccata aaacattgtg
    14101 ataaattagt tacttttaat aaactcataa ttcaaactcc ttttctttta ttaaaaaatt
    14161 tatggaaata aaaaaagcag aggaatggca ttgtaaaatg ccgacctctg cttttggaaa
    14221 aacaaaaggt catagacaag cattgttgtc tatgaccaga aaatctatac tatctatcgg
    14281 caaataggta attttaaata agcatagcca aaaaactata ctaaacctat atttgccgat
    14341 gaagatagaa agctttaagc tttcagatta acctctacac aacgcttcat cgtatacatt
    14401 tgtgtagagt taagttttct aacaagttta actaattcta tataaatcat aattattcac
    14461 atcctaattt tagtattaat tctagtttat ctatatttaa attgtacttt tgtactttgt
    14521 ggattatctt atttatttaa aatatgtttt attaattata gcttaagtta tgttatcata
    14581 atacttggtt atagtcaata agaaagttat atttgctata tattatatta atttagtaaa
    14641 tctatcatta caaagttatt tgaagactcg ctaccaataa aattttatag tctatgggaa
    14701 aattaagatt atgagtttta tacttttgat atagggatac tattttcata aatattacaa
    14761 catgtagaag tatatgtcta gtattgttga attttcaatg atatattaag gttctaatat
    14821 attatacaaa ataaaaacat taattcaact taaataattt taagtattat atgtgttttg
    14881 attaaagaaa actaaaattt taaggaaaaa taacatattt gtattaaatt ttattgaaat
    14941 gattaaattt atttacttaa tgtaaattat tagctaaaat tgtattaaat aattaaggag
    15001 gtgagtagct ctggatgaaa tagatttaaa aataattaat tctctaaaaa agaatagtag
    15061 ggcttcaacc tctgaaataa gcagacaaat taatttatca gtaccagcag tctcagagcg
    15121 tataaggaaa attgagcagt caaatttaat tcaaaagtac actattcaaa ttaatcggga
    15181 aaaaacaaaa tataagcttt tggcttttat atttatcaat actgatttat ctaaaagtat
    15241 gaaagaattt aagaaaacta tattggaata tgatactgta ttagaatgtc atcatatttc
    15301 aggagagtat gattacttaa taaaagtatt agcagaagat acaagtaatc ttgaagagtt
    15361 tatatcagtg actttgaagg aaattggggg gattcaaaaa gctaatacaa ccattgtttt
    15421 atctacaatt aaagaagaat ttaatacaat aaaagatatt ttttaaataa tttactagta
    15481 aagtgttaat tatattttag gaggaaaata gaatatgaaa gttttagttt ttttagcaaa
    15541 aggttttgaa actatggaat ttagtgtatt tgtagatgtt atgggatggg ctcgtaacga
    15601 ttatgggcat gatattgatg tagtgacttg tggatttaaa aaacaagtta tgagtacatt
    15661 taatatacaa gtattagttg ataagacgat agaagaagta tgtgttgatg attatgatgc
    15721 cctagctatt cctggaggat ttgaagaatt tggattttat gatgaagcat atgattcatc
    15781 ttttttaaat ctaataaggg agtttaattc aaaagaaaaa ataatcgctt ctatttgtgt
    15841 ggcagcattg ccagttggaa aaagtggtgt tttaaaaaat cgtaaagcta ctacatatca
    15901 tttgaaaaat ggtaaaagac aaagacagtt aagtgagttt gatgtaaatg tagttaatga
    15961 accaattgtt gtagataaaa atattataac ctcatattgt ccagaaacag ctccacatgt
    16021 agcttttaag cttttagaaa tgttgacatc tagagaacaa atggatgaag ttaaattagc
    16081 aatgggattt aaactgtaat gctctataga ctttctaagt aatcagaacc ccagtcacac
    16141 atggaatcta aaattggaat aatactcttt ccaaaatcag atagcgagta ttctactttt
    16201 ggaggaacaa caggataaac cactcttata attaaaccat ctgattctaa ttcacgtaat
    16261 tgttgagtta acatcttagg cgttgcttta ggaattaatt tttttaattc attaaacctc
    16321 aatatagtgt ctttaagatg ccaaagtata agtgtcttat atttaccacc aatcaatgca
    16381 agtgttgctt ctattgggca actgtaatta tctaaacatt ttttcatttt tgagctcctt
    16441 tatttatttt aaagtatctt tttagatact atattacaaa aaagtgcatt attgtaatat
    16501 agtaccatag tgataaaatt atagcatagg ttaaataaat catcaactta gcctaataaa
    16561 acaatagggg gtaaataaat gaatttcgtt gaacttacaa aaaaaagata ttcttgccgt
    16621 aattatcaag atagaaaggt tgaaaaagaa aaacttgaga aggttttaga tgttgcaaga
    16681 atagctccaa ctggtggtaa tcgtcaacct caaagattga ttgttattca ggaaaaagaa
    16741 ggtataaata aactttctaa ggctgccaat atatatgatg ctccattagc aattcttgta
    16801 tgtggagata aagacaaagt ttggacaaga ccttttgatg ggagacaact tacagatatt
    16861 gacacaagta tagttacaga ccatatgatg ttacaggcaa ctgaacttgg attggcaagt
    16921 gtttgggtgt gctattttaa tccagatata attagagaag agtttagttt gccagataat
    16981 ctagaaccaa taaatatatt attgatggga tatgaatcta agataccaga gagcccagag
    17041 agacatgaaa aaactcgtgt tcctttaagt gaaattgttt cttatgaaac tctttaatta
    17101 taaaattaag ttattcatag gataaatgta ataaagtgtt tattataaac aaaattcaaa
    17161 ataaataata tctacatata attaatttta gtgaagtata tctagtttaa ctagaatgta
    17221 tacttcacta atttatttat tcaaaatatt tatgaggatt ttaatatatt aactaaaata
    17281 tatcaattga attaactttt aaatctggaa aagcacttga aatatagtgt tttatgtcat
    17341 ttaaatggtc ttgaatttgc ttttctgata ttttatcagg tccatataca aaatacataa
    17401 tatttttagt atcttttgtt atagctgttc gattatcagg gccattaaga atactagata
    17461 atataccaat attatctgat aaaaataaat catcttttgt taaaatatgc tctttaccac
    17521 ctataccata aaaatgttca cttccatttg ctaattctat tttcaaaggt aattccattt
    17581 tatcaaggtc atgacctgct gttagaagaa gatttttcaa ttctgctaaa aacattgact
    17641 caattgttac acctacatct gggatacctt ttgattttaa tagaatagat tctaattgaa
    17701 gcaatacagg gtaagttttc ttgaattttt tataatactt tatataactt gaaacaggct
    17761 ctgttttaat tttttcttta cgattgtatt ctgggtgttg ttctataata tttttaattg
    17821 tagagttttt aagaactaaa aaattttctt tattaggttt tgaatgtgta atatttataa
    17881 ccataactcc aaatttaata ttgggataaa tttctttaac tttatgtgat atttctaaca
    17941 taatatagac tcctttttat tttatatttt atgatataat cttagcaagt taaaagtgga
    18001 ttttgtagtt ccatttttat agtaatttta tagaaccact tttggaggta ttagaatgtg
    18061 gattacgatt aatagaaata gtaatatatc attatcaaga caaatttatt gtgagataaa
    18121 agatatgatt ctggatggaa gcttaaagtt tgagcaaaaa ctgccatcat ctaggacttt
    18181 atcaaaagaa ctatctgtat ctcgtaatac agttttagat gcatataatc aattaattgc
    18241 agaaggatat ttggaaacaa ggaatggata tggaacaatt gtagctaaag gaatatcagg
    18301 gcataaagtt gattttaagt taaatgatac ttttagagat gaaaaagatt tacatactaa
    18361 taaacaaaaa aattatatag attttcgttc aggagttcca gaattaaaac tatttccacg
    18421 caaagaactt tcaaagctat ataaacaaat actgtatgat ttatcagatt cggatttcag
    18481 atatggaagt acagctggtg tatgggtttt aagagtggaa atatctaagt atttatttcg
    18541 tactagagga attagatgta attccaaaaa cattatgatt gtttctggtt caacacaagg
    18601 tttatcttta atttcacagc ttttatacaa aagtaataaa aaggttgctg tagaagaccc
    18661 tattcataaa ggcttattaa aggttatttc atctattgga tattcagttg ttggaattaa
    18721 agtagatgat aagggaatga ataccaactt aataaaatca ataaagggga tttcttttgt
    18781 ttatactaca ccttctcatc aatatccatt aggagggatt ttacctattc aaagaagact
    18841 agagcttatt aaatgtgcaa cagagaatga ttactatata attgaagatg attatgatag
    18901 tgaatttaga tatgagggtc aaccaattag ttctctttat gaacttagcc caaataaagt
    18961 aatttacata ggttctttta gtaaaatatt atctccagct attcgtgttg gatttatgtt
    19021 attaccagac aatattttag aaagctataa aaccttaaaa atgtactctg atgttcatac
    19081 agaaacaata ttgcaatatg tattagctga atttattgga aatggaggtt tagagaagta
    19141 catatttaga atgaagaagt tgtacaatag aaaacgtaaa tatctcataa aagttttaaa
    19201 cacggaattt aaaaatgaat ttaaaataaa aggtgaagca gcagggcttc atatagttgc
    19261 tcatttctat aatgttaaat ttacggaaag tttaattcat aaaatttttg aagcaggtat
    19321 aaaagtttat cctatacaac aatatgcaat tgagaatata ttagaatatg accatgaaat
    19381 tatactaggt tatgctcatc ttagttttga tgaaattgaa aaaggtgtaa aaatactcag
    19441 caaaataata catatgaatc aaggtacata gataaaacat gtagcaatct acatataata
    19501 gatgtaatta agtttaaact ttaaaagaaa tatatattaa tatttttgta taaaaatact
    19561 tgatattgtt ttatcgatac tttattgcta tacactaaat taagatagca atttatatta
    19621 agaaaaaact ttttgcatag gttataatac ttaaaaggag aggatagagt tgaaaacaaa
    19681 tacagatgtt attatggaga taattgatta tatagaagaa aatcttacag aaaatctaaa
    19741 tttagataaa atagcttata ttgctaatta ttcaaaatac catttacaca gaatgtttac
    19801 aaatgttgta ggatgtacta tccatcaata catacagagg aggagactta ctgaagcagc
    19861 tagacaactt atatttacag acaaatatat tatagatata gctattacag taggatattc
    19921 tacacatcaa tcttttacac tagcatttag aaatttatat aaagaatctc cacaagtata
    19981 tagaaataaa gatgaatttt atcctattca acagagattt gttttaaatc taaatcatca
    20041 aattcaaagc gaaaaatgta tttcttatgc aaatatgaat tataaacaaa gaagagatga
    20101 aatcaattct tatcttaact ttaatattaa ttgtgaggtg aaagtagcat gagtttagtg
    20161 ccacatgtaa ttgaacaaac aggtcaaggg gaaaggtctt atgatatata ttcaaaatta
    20221 ttaaaagata gaattatatt tataggagaa gaaattaatg atgctattgc aagtttagta
    20281 gtagctcaat tactgttttt agaatcagaa gacccagatt cagatataat tatatatata
    20341 aatagccctg gtggctctgc tacatcagga tttgctatat atgatactat gaattatgtc
    20401 agatgtgatg tatcgactat ctgtattggt atggctgcaa gtatgagtgc ttttttactt
    20461 gcaggaggaa cacatggaaa aagatgtgct ttacccaatt cagagattat gattcatcag
    20521 ccaatgggag gagctaaagg tcaagcaaca gatgtaaaaa tagctgtaga taatatattg
    20581 aaaataaaag aacgattaga taaaattctt tctgaaaata caggtaaatc aatagaagat
    20641 attagaagag atacagatag agataatttt atgactgcac tagaggctaa agagtacggg
    20701 ctgattgatt atataatgaa taggaatgaa taggaatgaa taaagtatca aagtatcata
    20761 ttaaaatgtc tgttttacaa atatatatat aatattaaat aagagtgtct caaaatgaac
    20821 tttttagttt atgggacact cttttttgta tgaaattaaa tatggtttta tcatttcaac
    20881 aattattacc aaatttcaag tttaataaat tctactataa aaaatacata aaagataagg
    20941 ttctttagta actttctgtc gtcaaaagtt tttataaatt taataacaaa acaagagaaa
    21001 ataccatata ttacttatgt ctgcaatata tagaagacaa aggagttgta cattatgatt
    21061 gcaataagtt tgtgcatgat tgttaagaat gaggaagaag ttataggcag atgcttagaa
    21121 tgtgtaaagg atatagtaga cgaaattata attgtagata ccggttctac agattccaca
    21181 aaagaaatag tctcaaaata tacagacatg gtgtacgatt tcgaatggat agatgatttc
    21241 tctgctgcaa gaaatttttc attttctaaa gcttctaaag actatactat gtggcttgat
    21301 gcagatgaca taattttaga agctgataga gaaaaattat taaagattaa agaaagcttg
    21361 gatacttcca ttgatattgt tatggcaaaa tacaatgtat cctttgatga aaatggtaac
    21421 ccaactctct catattatag agaacgattg tttaaaagaa gttctaatca taaatgggtt
    21481 gaccctattc atgaagtagt acctcttttt ggagaggtat tttattctga cattgcaata
    21541 tctcataaaa agcttcatag acaggaccct ctaagaaatc ttagaatttt tgaaaaaatg
    21601 atttcagaag gaaaaacatt ggagccaaga caccaattct attactctag agagttgtac
    21661 tataatgcta ggtataaaga agcaatagaa ggttttacta agtttttaga ctcatcaaga
    21721 ggatggattg aggattgtat cagtgcctgc agagatttag ccacatgtta ttacttaatt
    21781 aatgatgaaa aaagtgcctt atattcattg tttagaagtt tcgaatttga tgagccaaga
    21841 gcagaaattt gttgtgacat aggaaagcat atgtttgata gacaaaaata taaagaagcc
    21901 attttttggt ataaggtagc attaacaaga gataagaatg atactaatgg aggttttaag
    21961 tcaaatgatt gttatggata tataccatct atacaattaa gtgtctgtta tgacaggtta
    22021 ggagaatcag ataaagctat ttattatcat gaaaaaacaa aagaaatcaa acctaacgat
    22081 agtgctgttt tgcacaatga aaattatttt agtaaattta aaaatagttg ataggtcaca
    22141 atcatactaa atttgacata ttatatatag agcttaaaag ctcaaatata tcaggaggtg
    22201 cttttaataa tgagtagaaa taaatatttt ggaccatttg atgataatga ttacaacaat
    22261 ggctatgata aatatgatga ttgtaataat ggtcgtgatg attataatag ctgtgattgc
    22321 catcattgct gtccaccatc atgtgtaggt ccaacaggcc caatgggtcc aagaggtaga
    22381 accggcccaa caggaccaac gggtccaaca ggtccaggag tagggggaac aggcccaaca
    22441 ggaccaaccg gtccgactgg cccaacagga aatacaggga atacaggagc aacaggatta
    22501 agaggtccaa caggagcaac agggggaaca ggcccaacag gagcgacagg agctataggg
    22561 tttggagtaa caggcccaac aggcccaaca ggagcgacag gagcaacagg agcagatgga
    22621 gtaacaggtc caacaggtcc aacgggagca acaggagcag atggaataac aggtccaaca
    22681 ggagcaacag gggcaacagg atttggagta acaggtccaa caggcccaac aggagcaaca
    22741 ggagtaggag taacaggagc aacaggatta ataggtccaa caggagcgac aggaacacct
    22801 ggagcaacag gtccaacagg ggcaatagga gcaacaggaa taggaataac aggtccaaca
    22861 ggagcaacag gagcaacagg ggcagatgga gcaacaggag taacaggccc aacaggccca
    22921 acaggggcaa caggagcaga tggagtaaca ggcccaacag gagcaacagg agcaacagga
    22981 ataggaataa caggcccaac aggggcaaca ggagcaacag gaataggaat aacaggagca
    23041 acagggttaa taggtccaac cggagcaacc ggagcaaccg gagcaacagg cccaacagga
    23101 gtaacagggg caacaggagc agcaggacta ataggaccaa ccggggcaac aggagtaacc
    23161 ggagcagatg gagcaacagg agcgacaggg gcaaccggag caacaggtcc aacaggagca
    23221 gatggattag taggtccaac aggagcaaca ggggcaacag gagcagatgg attagtaggc
    23281 ccaacaggtc caacaggggc aaccggagta ggaataactg gagcaaccgg agcaacagga
    23341 gcgacaggtc caacaggagc agatggatta gtaggtccaa ccggagcgac gggagcaaca
    23401 ggagcagatg gagtagcagg tccaaccgga gcaacagggg caacaggaaa tacaggagca
    23461 gatggagcaa caggtccaac aggggcaaca ggtccaacag gagcagacgg attagtaggt
    23521 ccaacaggag caaccggagc aacaggatta gcaggagcaa ccggagcaac aggcccaata
    23581 ggagcaacag gtccaacagg agcagatgga gcaacagggg caaccggagc aacaggtcca
    23641 acaggggcag atggattagt aggtccaacc ggagcaacgg gagcaacagg ggcaacaggt
    23701 ccaacaggcc caacaggtgc tagtgcaata ataccttttg catcaggtat accactatca
    23761 cttacaacta tagctggagg attagtaggt acaccaggtt ttgttggctt tggtagttca
    23821 gctccaggat taagtatagt tggtggagta atagacctta caaacgcagc agggacattg
    23881 actaactttg cattttcaat gccaagagat ggaacaataa catctatttc agcatacttc
    23941 agtacaacag cagcactttc acttgttggt tcaacaatta caattacagc aacactttac
    24001 caatctactg caccaaataa ctcatttaca gctgtaccag gagcgacagt tacactagct
    24061 ccaccactta caggtatatt atcagttggt tcaatttcta gtggaattgt aacaggatta
    24121 aatatagcag caacagcaga aactcgattc ttactagtat ttactgcaac agcttcaggt
    24181 ctttcattag ttaatactgt agcaggatat gcaagtgcag gaattgcaat aaattagatt
    24241 agataagaaa aggcttctaa tatcgtattg atattaggag cttttttaag aatgattgat
    24301 gaacatagtt ttaatattaa ttagtgcgca tattataaaa tactattata taatataaaa
    24361 taataacatt aaattggaaa tatagaatta tctaggggtg atactatgaa aaataagatt
    24421 gaaacatcaa tgaacaactt tgtaaaaaat tatcataaat caaataatat aactacaaag
    24481 tggcaagaac ttttgtttgc ttatgcatct gctgaagatg atatgtttca gaagctaaaa
    24541 agtgttgtag gaacatctca tgctatgcca aaagattttc tggaaaatgg aaaaactata
    24601 attacatatt ttataccttt tgatgaaagt gtaaataata gtaatattgg agggtttgaa
    24661 tgttctgaaa tatgggcaag agcgtacata gaaactaata tgttgatttc aaatttaaat
    24721 gatttcttat gtgaagaact agagaagatg ggggctaatt catatgctat tcctgggtcg
    24781 tataattttg acagtgaaaa attggtaagt gactggtcac aaagacatgt agcattcata
    24841 gcaggtcttg gaacatttgg tttaaataat atgcttatta cagaaaaagg gtgttgtgga
    24901 agaataggaa gcataatcac aagtttagaa ttagagccaa ctaaaagacc agaaaatgaa
    24961 tactgcttgt ataaacataa taatagttgc aaaaaatgtg tcaacaaatg cgtgaaagaa
    25021 gctttaaaag aagatggatt taataggaat aagtgttatg aaatgtgtat gataaatgaa
    25081 aaaaaataca cttatatagg tggttgtgat atttgtggaa agtgtatagt tggggttcct
    25141 tgttcggtaa taaatccctc aagataaagg acttatgttg attaataact ttaaatatat
    25201 ctaataagaa gaaaaaagtt agtctctaaa atagatgaat tatgaaaata aataaagata
    25261 atataaaggt tagatgaaga agttatgtta attgacaact atttatactg ctgatagact
    25321 tatatgtatc gttgcaatat ataaggaggg gtgagattta tgtttgattt aatctttatt
    25381 tttgcatagc aagggctatt gcaaatataa aatttaatat ataaatagcc ctttgctatt
    25441 cctaataaaa aattattatt taataggagt gcagaatgag ctataaaaaa gcaatacata
    25501 tattaccaga agaattactt gaattgatac aggaatatgt agatggagaa tatatctata
    25561 ttcctagaaa gtctaataat aaaaaagaat ggggaagtag tacgtctact cgtgaagatt
    25621 tatcaattag aaatatgcaa atttatgaag attatcaagt tggatatgat ttgaaatatt
    25681 tatcagaaaa atattacttg tctttgaaaa gtattcaaag aattgtatta caagaaaaaa
    25741 gaaaagatag ttaagaatga gccagtatag ttttataatt atactggctc attttctttt
    25801 gataaaagtg tttgaggtat agtttcttat aagtggatgc tataatttaa ataatttcaa
    25861 taacttgtac agcagataac gaatagagtt gtaaagtatc taataagaag tagtagcttt
    25921 ttatatgttt gaaattaaaa tttattaaaa ttaggaggtc ttgttttgaa aaaaatactt
    25981 gattatattc caccttttca taactttcta ttaatttagt aatcttatag aaaggaagtt
    26041 gcatttatga aaaaaattac ttgggaggtg gaatatatct cccagttacc tgtaattagg
    26101 tattgtaaga aatgtggaaa aaagactgaa tatatttgtt caggtttatt tagggttaat
    26161 gctcaacata aatatttgga tatatggtta atatacaagt gttcaaattg tgattcaaca
    26221 tggaactcaa ctatatattc tcgtgtcaat ccaaaaagct taaatccaga aacactagaa
    26281 caattccata caaacgatta taatcttgct aagcaatatg ctatgaatac ggaactttta
    26341 catagaaatg gagcagaagt tggtattcca gagtataaaa tagtaggaca agaaattaat
    26401 attaatgacc ttacacaagt tcatattatt agtaaatatc catgtcagtt aaaggtatca
    26461 acattattac gtgagaaact aggtttatct aggaaggtat ttgacgaaat gttggattgt
    26521 gggattatta gaagtaactc aggagttgat ttaaagaaag gtaaattaaa ttatgaaatt
    26581 atgttagata taggcaagga gaagcaaaag tcttcaaatt gattagaata actaacttaa
    26641 tatattttca ggaacatcat ttaaaaaaat agtggcttta aaaaatttaa agccactatt
    26701 ttttaatatg ttt
//
LOCUS       DACTGW010000030        26372 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.030, whole genome shotgun sequence.
ACCESSION   DACTGW010000030 DACTGW010000000
VERSION     DACTGW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.030"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            535..750
                     /locus_tag="I9573_001001"
     CDS             535..750
                     /locus_tag="I9573_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HAT4795630.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKSYIVEDLIQVLEEKGVM"
     gene            750..1229
                     /locus_tag="I9573_001002"
     CDS             750..1229
                     /locus_tag="I9573_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HAT4795631.1"
                     /translation="MIVYHDSYCVAKTPNIIFLRDSLDFGKGFYVTSIKTQAINWIER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFILECRNMSDVYMKYDVIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRCEEEV"
     gene            1235..1465
                     /locus_tag="I9573_001003"
     CDS             1235..1465
                     /locus_tag="I9573_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795632.1"
                     /translation="MEANKTLLQILYKKIILEFSNQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(1581..2747)
                     /locus_tag="I9573_001004"
     CDS             complement(1581..2747)
                     /locus_tag="I9573_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAT4795633.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHMQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENRLEILTLEQQKNFIKAIEGHKLEVLFLVALGTGLRLGELLGLKW
                     FDIDFKNSNLTVKRTLQRTYFIDKNGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNVDILKAGELYRNDNYVFCNELGVPIDDKRPLRNLKSILSSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(2737..3489)
                     /locus_tag="I9573_001005"
     CDS             complement(2737..3489)
                     /locus_tag="I9573_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795634.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYIIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNNLFISDISIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(3581..4072)
                     /locus_tag="I9573_001006"
     CDS             complement(3581..4072)
                     /locus_tag="I9573_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4795635.1"
                     /translation="MINNTIGEKIKQYRKSINMSRNELANKIGCSVHAIAKYEQNQRI
                     PSLDMIRKISTALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVVHILENYGYDI
                     KTDYKKKFEITDNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            complement(4069..4266)
                     /locus_tag="I9573_001007"
     CDS             complement(4069..4266)
                     /locus_tag="I9573_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795636.1"
                     /translation="MNKAYMLKYTYKYPTNLFYGDKKYTLTYIKRIMSKLVYDIDNLE
                     NNSFICVKIQSLFDDIFKVEL"
     gene            complement(4323..5087)
                     /locus_tag="I9573_001008"
     CDS             complement(4323..5087)
                     /locus_tag="I9573_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4795637.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCVSNTELDFSLHPDDI
                     KEIIKNDVLIKQSIDELLDIGLSEENIEAIYDYFSEATDNGSNIRLKKLISQKENMTI
                     KNNDYFKLLQENNELKEKNKKLENTLKEIKKLLTKI"
     gene            5234..5434
                     /locus_tag="I9573_001009"
     CDS             5234..5434
                     /locus_tag="I9573_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4795638.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKEE"
     gene            5438..5752
                     /locus_tag="I9573_001010"
     CDS             5438..5752
                     /locus_tag="I9573_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795639.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            5774..6517
                     /locus_tag="I9573_001011"
     CDS             5774..6517
                     /locus_tag="I9573_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795640.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSIIREYDKEAVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYHSKEIVINKETGIYNPDDSQLKW"
     gene            6530..7864
                     /locus_tag="I9573_001012"
     CDS             6530..7864
                     /locus_tag="I9573_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4795641.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVEELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFTSY
                     VSIVIEKYKNRNIIDKFNKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEMNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDDEIVKYIQALSIINQENNLY
                     INDSVNTVSGIKREIKSTKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            8114..8386
                     /locus_tag="I9573_001013"
     CDS             8114..8386
                     /locus_tag="I9573_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795642.1"
                     /translation="MYDEIIEIYFDYWINEHMYRSILEKDEYYIECWKEVKKINELLE
                     INLEDDKKYLLVELNDLFNSLIEFERELSYKLGFKTGAKFIIELLK"
     gene            8659..9786
                     /locus_tag="I9573_001014"
     CDS             8659..9786
                     /locus_tag="I9573_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAT4795643.1"
                     /translation="MIIPKLEPNDRKREFNKYSSEQINKVVYNYLFKNNMSNRKIDDE
                     ILDLSSKQSKGYQSMGIVHHLGINKNHKGIFTGYTIDKAIEVLKEDEIHFKQIINILL
                     EIRNINEYPIIVDSWELLDEKIALKTIDKSTLKHNGTGVPIEIRYFFEINNLGKNEKK
                     NVNFKYNDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRSIFSEYYEKFLENKIEGKTN
                     LPKIRFIKLDSNNFNIEFIFTAEIEDDVLEEISQDNPLNINTINIELETEIRKEGKVK
                     YVYGKQYERNPRNRIEAIKYHGTKCIVCGFDFEKTYGDRGKGYIEIHHIKPLSSVGEE
                     SNINPKTDLVPICSNCHRMIHRKKDNVLSIDDLKQIIKDSK"
     gene            10266..10913
                     /gene="cas5"
                     /locus_tag="I9573_001015"
     CDS             10266..10913
                     /gene="cas5"
                     /locus_tag="I9573_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HAT4795644.1"
                     /translation="MILNKIEIIGDMAHFKIPMHSKIQRTYEIPPISTVVGILKNIYG
                     EEIDDFILGYVIYHDGTFKDLQTIYKEINPNVKTLTDSDRFQTDVCPIEYLINPKLII
                     YTNIDKNIEFNEPLCLGKTNCLAKVLSLSEIEVNLIDKKAIGYNQYTDINIGDGMIKR
                     INTLTEYNEKKGYYDIYSSMVRENDEFEYGKYYDEDEQQNIFLWKWKKGGVIKCI"
     gene            10904..11668
                     /gene="cas6"
                     /locus_tag="I9573_001016"
     CDS             10904..11668
                     /gene="cas6"
                     /locus_tag="I9573_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HAT4795645.1"
                     /translation="MHLVRRHLILTVDNEVVLDYNYQYELMKRIYEAIEINDKRKALS
                     LHNEGYKVDKKVFKLFNYTIMFENAKYLKEGIHLNPQTKIKLILSGYDDILNNIIKGF
                     IKCKVFKLNNIEFKVSDIEEDSKKNFNNITLYKVRSPIVASLYDLKSRKQVYLNPMQE
                     EFYKALHDNLGNKYKLIHNKEYTGELYFDIEDVLAVKKKYITNIKGKGFIIGYTDFEI
                     FVQANKDMQEVIYYCGLGEKNSIGMGLLTYITSRRA"
     gene            11670..12836
                     /locus_tag="I9573_001017"
     CDS             11670..12836
                     /locus_tag="I9573_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795646.1"
                     /translation="MDRSYNVFVDNGLYVLAYYLNKDINDITYQDIENSIDLMSDKIE
                     EFVSCEKYSNLKSMCFSNSTLTQPKGKVTLNEKLKGFVKNQGDEYCSLCGQYKAKVKI
                     EDKEYNIGRSYMPNLVANTFYNFSNNLQGLNVCPYCLVLTMYSILNCRVSRYAFLYNS
                     TSSEFMEDYTFHIQKENLTDVELGAKKEKEKYSIVKLLESLVCKYNLFNGDIEQYMFN
                     NSGQSQDIQVNRIKNKYVNLLIKLQEKALLSNFEKLHLDRYILNGTLESNYLREVYRV
                     KKEEKMDEKEQKNFDELVIFLNREVNVLKKVKIDIIKKLTDKLKTSDLDIDKIYKELK
                     MLGKFYAYEDWLVNLNEMYYEKTGEKLFEAEDYIELDDIRSFRQIKNLILVSLI"
     gene            12857..13927
                     /locus_tag="I9573_001018"
     CDS             12857..13927
                     /locus_tag="I9573_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HAT4795647.1"
                     /translation="MENKNSNRMLNIGYAIEVLCGRLNGSSSEVEKKNNSYKNSTYTK
                     KIGSRSRISSQCQKYNQKLFMEQVCGYEQNVKTKDGKQVKLVPNPFKYATDDIFGFMM
                     AEKLSIEEDVYKELSDEEKEIYKKNKKKYEVNITKKRKSRWQMNSLINVSNRKVEWEW
                     NVCGTTGDSMPYQVEVYSGVHTNIANLNIGKIGEYDISDIASEHRDYTTLEAKSIGVE
                     KLDKEEKFKRIEALLKSLEYLSVEGNKTNHLTDTKPKFVILGEYSWGNNVFQGIIKEN
                     GLDVEMLKESIEQNEEFRKSDIWIGINKFYDDTFNEIEVNNEKEQIKKYLEEEFEEYD
                     FMKISNVHQAFESYLKYLKETL"
     gene            13938..15923
                     /locus_tag="I9573_001019"
     CDS             13938..15923
                     /locus_tag="I9573_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HAT4795648.1"
                     /translation="MNNLYEYKAKSDGTSIDKHNEDMNYIMNQLVSIYNISNDMKNDL
                     YEAIVCHDVGKVVKSFQDNIESSTRRVRHELLSASIKYLNRGQKLAILTHHKDIKFLE
                     GQITNSIYEIELKELKELLDIEVEDIRPFIKEISRARNDDTRKLENILLKGYLQYCDH
                     IASAGIKSIDKGFNSIKDFSIPKGVSYNSIQEKVLNMDKNEDIMIMAMTGLGKTATSL
                     YWSNLVQNEYKSKRVYYILPYTASINSLYENLYNRNISVSMLHSKAPYFLNKISDEED
                     IKQLYQIYKKSVKQINVCTIFQLVKSVFSCKRFEMLLSQLKDSIFIVDEIHCFDTRVL
                     CYTLEFLKYLKENLNVKICIMSASIPTCLKKLIQERLQISTIIEPDSKDLIFRHRLNR
                     LSEGILDSIHKIENDLMQGKRVLICVNSVDLSQELYGRLQKYNPKLIHGRFNTRDREQ
                     AEKGIEESFLLIGTQSIEVSLDISYDVLYTELAPLDSLLQRFGRVNRRGDNAELSNIY
                     IYDYDRKLNIYDEDIINRTDIVISDIIHNDNSIVDESKVPSYLDNVYLDIDMDKYNKH
                     KVALEIIMKNMRLGVWNKSANNDMLNNDTISVVPECLLEEYKLYIDKKMYLEASSLFV
                     NVSVKRYNYNKKMFYECGDIVVTSYSYNDKLGLIFDC"
     repeat_region   16176..16798
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=16176..16204
                     /rpt_unit_seq="gtttaaattacactaagttagttataaat"
     gene            17243..18790
                     /locus_tag="I9573_001020"
                     /pseudo
     CDS             17243..18790
                     /locus_tag="I9573_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706893.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            19977..21191
                     /locus_tag="I9573_001021"
     CDS             19977..21191
                     /locus_tag="I9573_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HAT4795649.1"
                     /translation="MGEKMQSVICKIFDNKLLISNDLSEQDDVLYEVMDKFIDDIEYI
                     SKLGDEQIKSMLMEVNKFEEDSTEFELYMKNLCYLKQSYFIDTNDKSEINSIDIDILK
                     NVKIEDLVDITNIKLERNLNINIEHISKAVNKIYEENEKWKEYNIQNTMSKYIGIPES
                     EVIFISNLSQYVSFLSELDNAGDYVSRGQKDCMYELLPSLHRKYKKDYGIHSDEYEEA
                     FKQKIIYYDKEIKNRSSEELRAEGQHFGLPTEYLDFTEAHLISLLFAIEDYKYTKQHS
                     IVYFIDSISYNTDTIKRSEKLVNYSDTSAIDNCSKFSSRSFFIKVGNSNERIHFQKGC
                     FLKVSSEDKGEFKEKLKKYCKVVVINKDAKKEILKELFNLGITFENIYPDKDNVVKSI
                     KFYYDEIVGGNL"
     gene            21191..22483
                     /locus_tag="I9573_001022"
     CDS             21191..22483
                     /locus_tag="I9573_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAT4795650.1"
                     /translation="MREQFKNSIKNIKSNRPLVSVQDIVNFIDKGEKYLKLSIDEIDE
                     MEILQGIVMSPDYQRTYKSSNKEESSIIESLLIGIPIPEIFLVKIGVNDMQIRHVMDG
                     QHRLNAIYRYVKNKYPLKNLDILGNDPLYDNKRFSELEKKDKIKILGSHLSILEFDGF
                     DNPEIEIELFKRYNRNTKPLEMQEIEMATYFSETSKYISKFINTLIKDKYKDEIDKSE
                     YKYANYKSDLLRIYNITKSRNDKQKNHQEICIIFSILENGLQENIRDGVTASKKFLEY
                     KSIQYKNNESEKLELLKDKFDEFNRMILKISEKIEYPFSTSLISDDKQRQSKYLIGVS
                     IVLASICYYYDIDLENNYLIQDIKKIISCSPIADIEYKASSTNMKNMILYLFIKNKIQ
                     DNKFESLNLKRDKIKEINTLVMNNDGYLNEQTRLLEIY"
     gene            complement(22710..22895)
                     /locus_tag="I9573_001023"
     CDS             complement(22710..22895)
                     /locus_tag="I9573_001023"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAT4795651.1"
                     /translation="MEGFWRILKVEMYYLRKLDTYEQLVKTIEDYIYYYNNFRYQKRL
                     NSMSPLEFRKYLNTEIA"
     gene            complement(22925..23314)
                     /locus_tag="I9573_001024"
     CDS             complement(22925..23314)
                     /locus_tag="I9573_001024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAT4795652.1"
                     /translation="MRAVGLSSVCRKKKFSYVKCTPEVIAENVLSRKFSADKTSQKWL
                     TGVTEFKLTNGMKAYLSAILDLDDRSIVSYVIGKSNNNNLVLETFDKAIELYPNAKPI
                     FHNDKSFQYTSKVFKSKLLTQGMIQSM"
     gene            complement(23416..23643)
                     /locus_tag="I9573_001025"
     CDS             complement(23416..23643)
                     /locus_tag="I9573_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT4795653.1"
                     /translation="MDKRTFREWMRNYEAFGNDGLIKQSCKLCYCEEFKLNAIKSYLN
                     GEGSYDTISKKYKMRSVYSLKAWGKEYNPHK"
     gene            24215..25225
                     /locus_tag="I9573_001026"
     CDS             24215..25225
                     /locus_tag="I9573_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795654.1"
                     /translation="MITKIKKTYIYLKEIQKIYKINEHKELVHIIKKLINDNKIKPIK
                     TSDLTPQHEQLYTKYRIIKQETEEDKKILEEINYKICDKLSIDYYRKNLVHYKNNRVA
                     IMDLNSYLIKKSDNLSKKISINERSYEIFKDEKFISSKVGKEVLKNLKIDLIKDLNVY
                     KTPEPFIYLSINRISPQKILIIENKDTYITITKMLLEGKEILKNKIDTVIYGEGKKII
                     NSIKGLKEDITIEYLTNKENEFLYWGDIDKTGLSIYTSLKDTNEINNIRLFEVAYEHM
                     IDKTKENTLRNIPKNQKDNFECGLENINNLNLKESIKNILVQGKYIPQEALNRFDLEE
                     YN"
     gene            25225..>26372
                     /locus_tag="I9573_001027"
     CDS             25225..>26372
                     /locus_tag="I9573_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795655.1"
                     /translation="MEMINILEGYNKRVNCIGSYYLIATLANNRNKFKEFDNIQFYNL
                     LIQVLCYIFDRSLRRKNCLRDDIKDFIEEINRMDYKIMLSEDDLKDLANYIINGLTNS
                     GKVYLFTYYSLEQEKHIDESIKIIEDKNVKINNQERLSYSLTTEGYRLLLSTKEYDEL
                     FQIQISQMIAKLRIEKGDYQGAKEDIYDIINTLEIHQQKIDNYIKIIKVNILYIKEEK
                     YSTVIDTTVETLLEEMDKYEELKKEVKLLIKDKEDYLEKDEDIDKEKIKNIRTQLLQM
                     KELIDGIVLAKSTATNLLSRVQDFREEFKEILKKLLTTPVIKRFNFKEEILDRLEKDS
                     DFLLIIRELYISLFKVELPNRFNIGVAYQEQKLLNTREIENEIVNKDE"
BASE COUNT        10877 a         2654 c         3960 g         8881 t
ORIGIN      
        1 taagattata taaaaaaatc ttataagaga catctgttgg aaagttttta taaatatcaa
       61 agtcttttgt cataatttaa ttatatcatg ttgtagaaat taatttactg tatttaagtg
      121 ggtcaaattt aatatatatg tttttgatag tgtaaaaatt aaaatataat tttaggcatg
      181 tttgtattga ataatgagca ctttcaaatt ttattagtgt aagaggtttc aaattataaa
      241 gtaatgaatg tatattacta tatgatttat aattagaata aaatttttat aatagaaatt
      301 taatatatca aaatatcagt tgaggatatt gttcaagtgg tatatcttcc ctgagttaaa
      361 aaataaatga tgaaatattt ggaaatgcta gtctttattt gtttatgata ttatgtaaca
      421 tagaatttta atatctactt tatgatataa tatttttata aacataagat ggtttaagtt
      481 attaattaat aaaaatatat atattatata agtggatagg agttgaggtt tgtaatggat
      541 gaaagaaaac tagaattttc tattttttgc attgaagctt tagcagaaaa gttgaatata
      601 gattcagaag gagtttataa acttcttaaa catgatactg atattttaga taactatata
      661 ataccttgct atgaagcact acattctcaa agtaaaagtt atatagtgga agatttaata
      721 caagtgcttg aagaaaaggg cgtgatgtaa taattgtata tcatgattca tactgtgtgg
      781 caaaaacgcc taatattatc ttcttaagag attctttaga ttttggaaaa ggattctatg
      841 taaccagtat aaaaacacag gcaattaact ggatagaacg ttttaagaaa aggggtaaaa
      901 aagcatattt aaatagctat tctctagata ttgataaatt aaaagaaaac tgcaaggtaa
      961 aaatcttcaa tagttataat ttagaatggc ttgactttat attagaatgt agaaatatga
     1021 gtgatgtata tatgaaatat gatgttataa taggtggaat tgctgatgat aagatttaca
     1081 atacaataga actgtatcaa gataatctta ttgaaaaaga tgaagcttta aaaagattaa
     1141 aatactataa gccaaatgaa caaatatgta taactaatca aaatgtaata gataaatatt
     1201 taaagtttag atgtgaagag gaggtatagt gcttatggaa gcaaataaaa cattattaca
     1261 gatactatat aaaaaaatta tattagaatt ttctaatcag acaggcaaga gtttggaaga
     1321 aagtatggat tatttatata cttctgaaac atataaactt ataagtgagg gtgtatcaga
     1381 tatgcactgt aagggtcata tatacttggc tcaagaatta atgctagaaa atggattaat
     1441 gtatcataaa ggctatccaa gataaatagt taaaactaga tacgaattaa ggatacaaga
     1501 taatataaaa ttaatttgta tccttatttg ggttttgtaa ttaactatat gaaatcatag
     1561 tatttttatg tatttgattt ttacaatgaa aatattttat ccaacttttc tacagcttca
     1621 accttcttat cttccataac ttgagtatat atatttaaag ttattgatat atcataatga
     1681 cccattaaaa cttgtacagt tttaggagga acatcattct caaaaagtct agttgcataa
     1741 gtttttctaa gaccatgaaa ctttataggt tctatatcta agctgcttaa tatagatttt
     1801 aaattcctta aaggtctttt atcatctata gggacaccta attcattgca aaatacataa
     1861 ttatcatttc tatataattc tccagccttt aatatatcca cattttgatt tactttatgc
     1921 tctttcaatt tatttaaaac atcttttgga ataggtacag tcctgtaaga atttgatgtc
     1981 tttggttctt gctctaatac tttaagctct ctatttccat ttttatctat aaaatatgtt
     2041 ctttgaaggg ttcttttaac tgttaaatta gaatttttaa aatcaatatc aaaccattta
     2101 agtcctaata attctcctaa tctaagtcca gtaccaagag caaccaaaaa taatacttct
     2161 aatttatgcc cctctatagc ttttataaaa ttcttttgtt gttcaagagt caatatttca
     2221 agcctatttt cctttttctc aactggtata tttaccatag tacaataatt acgttgtata
     2281 agaccttgtt tttcagcttc tgataagcag gttttcagtt tagtgtttat ttgccttata
     2341 gtaggtatac ttttattgga atcaagcaat ctattgtaat atttttgcat gtgagatact
     2401 cttaagtcct ttaacttaac attgcctaaa tcagtatctt taatataatt acgatagatg
     2461 ctttcatatc tttcaaatga ctttggtttt aaatctttta ttctatattc aaacaaccaa
     2521 ttgtaaaacc aatgctctaa agttatttta tcatctgaaa tatcagaact taaataatat
     2581 tgttttttat aagcatcaag tttatcttta acttcctttt gagttttacc ataaaattct
     2641 tttcttattg gttttccatc ttcattataa ccaatcatta tacgagaacg ccaaccatct
     2701 ttatattttc cgatagagcc ttctccatta gctcttttag ttgccatttt taaacaactc
     2761 cttatatgat ttatagttat atccatcgaa gaatgtaata catttctcta ataagaattt
     2821 tataacttca tcagcttcta gtgtttcata gaagtttagc attaatgtgt tgtattcttg
     2881 tttatgtttg gctggtattg atagaactcc tatatcatta cttataagta ttaggtttga
     2941 ggtaagtcga gcagttcttt tgttaccatc atagaagaat tgacaatagg acaaccataa
     3001 atttattatt atagcctttt ctaaggggtt agttatttta tttatgatac ttatgtcact
     3061 tataaataaa ttatttaata gagtggcttc tatgcactca tattgagtag taccagctat
     3121 acctatgtta ccgtttctaa aatctccaac aattagagct tcatcttttg caactatttt
     3181 atgtatagag caaattgtat ctttatttaa agataagttt tctttgttac ttaattttat
     3241 tatataatcc catgatgatt ttatatttag tatttgattt tggtcactta atttatgtcc
     3301 acctactgtt ataccatcaa gtaatgtttg tacttctggg aatgtgaagg ggttattttc
     3361 taggttaccc atatcatata caaattcact tatcattctt ctaatgtatg ttaaagtata
     3421 ctttttatct ccatgaggta gattcgtagg gaatttatgt gtgtattttg gtatatatga
     3481 tttattcatg attttagctc ctttcatatc taatttttat ttattaacta tgaatgtata
     3541 aaagttaata ggtaaaccca tattttgaaa atgtgcaata ctaatattct tcatcctcat
     3601 cttcaataag accattagag ttttcttgta taaaattttc aacaagacca tttatcaccc
     3661 aatttagatt tttggagaag tctttgaagt cagagaagct taagctaatt ggttttattg
     3721 tatctgcatt atctgtaatt tcaaactttt ttttatagtc tgtttttata tcataaccat
     3781 aattttctaa tatgtgaact actaaatcca actctttatc aaatactttt aaattaatat
     3841 tattatttgt taaagtatct aaatcatttt cttcaacaat gagtttattt atggcaattg
     3901 ataaagcagt agatattttt cttatcatat ctagactagg tattctttga ttttgctcat
     3961 atttagctat agcatgaact gaacaaccaa tcttgttggc tagctcattc cttgacatat
     4021 ttatcgattt cctatattgt ttaatctttt ctccgattgt attatttatc ataattccac
     4081 cttaaatata tcatcaaaca atgattgtat ttttacacat ataaaggagt tattttctag
     4141 attatctata tcatatacta acttactcat aattctttta atgtatgtta gagtatattt
     4201 tttatctcca taaaataaat tagtagggta tttatatgtg tattttagca tatatgcttt
     4261 attaataatt gtaactccat ttgtagtttt ttatcattgc tttagatagt attgatatta
     4321 ttttatattt tagttagtag tttttttatt tctttaagtg tattttcaag ctttttattt
     4381 ttttccttta attcattatt ttcttgtaat aatttaaagt aatcattatt tttaatagtc
     4441 atattttctt tttgagatat taactttttt agtcgaatgt tactcccatt atctgttgcc
     4501 tcactaaagt aatcatagat tgcttctata ttttcttctg atagaccaat atctaataat
     4561 tcatcaattg attgttttat aagaacatca tttttaataa tttcttttat atcatcagga
     4621 tgtaatgaaa aatctaattc agtattagat acacaatctg aaagtatcca attataaact
     4681 tgattttcat ttagttctaa tttttcacca agtttataat attggtcttt atcagaccaa
     4741 gcacctagca ataaacttga ggtaatccta atatcatcat cttccataag atttgaaata
     4801 gcatttaaaa attcatcaaa agaatagtat tcttctggga ataaagactg taaaaaatat
     4861 aaaacatctt cattagtcat tacttcattt tcaatatcaa aataaaagat agtcttttca
     4921 atttttaaag ctttagcgag ttttgataag gtatctatac taggtattct tttatcattt
     4981 tcataattta ttagagcatt tcttgagatg ccactaattt cagcaagttg aatctgtgtt
     5041 agttcttttt gttttcttat cttttttaag ttttttccaa tttgcaatat tatcacctcg
     5101 tataaagtat atcccaaaag tacacaaatg agaacagaaa aataatatat tcttattgac
     5161 agcacacata tgtcgacatt ataattaaaa tgtaaagttt acgaatgtaa actagaaaaa
     5221 aggaggaaaa agtatgggaa tcaatttaaa aatatttagg attaaaaaag gtttaaagca
     5281 acaagaactg gcagatagag ttggtatatc aagatattat ttgtcaaact tggaaacagg
     5341 taaggctaat aatccaagca atgatttgat gattaaacta tctagagctt tagatacaac
     5401 agtagaagaa ttatttttta gtaaggagga gtaaaatatg aaagagaata ataatttaga
     5461 agttttaaag acattacaag ctactttaaa cattattgta aagaatgaag aagaagcaca
     5521 agaaaaaaag caaggatata aaattattga aaatgcgaaa agaaataaag ctgaacttat
     5581 aacatgtaaa gaagcatcta agctttatcc tataggagaa tcaaaattta ggcagttatg
     5641 tcactctaaa aataaaggtt tcccttgtat atggataaat aatcgagtat acataataaa
     5701 aaacaaattg gatgattggt ttattgaaaa tgcaaatggt atcaagttat agtcttaaag
     5761 taggtgtttg caattgagca ataaagggtg gataaagctt tacagggagt tgttggataa
     5821 atcaatttgg aaagatatta aaccagaaag aaaagtaata ttaataactc tattacttat
     5881 ggctagtcat aaagaaaatc atgtttttat agataataaa aggataaaaa taaagtctgg
     5941 acaatttatt tcttcttata gcaaaatagc tgaaagatgt ggtaaaggag ttagttatca
     6001 aatggttaga agttgtttgt tggtttttaa aaatgctggt tttctaacat acgaaacaac
     6061 aaacaaatat atagtcataa ctattgaaaa ctgggagctt taccaagaga acatagaaaa
     6121 tcaacaacct aatcaacaga cgattaacaa tcaatcaaca acaatcaaga atataaagaa
     6181 tgaaaataat attatatata gtgaaattat aagcaaattt aactcaactt gtattgaact
     6241 ttcaaaggtc aagaaactaa cagacacaag aaagaggaga ataaattcaa taataagaga
     6301 atatgataaa gaagctgtat tagaggttat aaatatagtt agtaaaacaa gttttctgaa
     6361 tggaaataat aatacaggat ggaaagcaaa ttttgattgg ataatgaatt caagtaattt
     6421 taccaagata ctagagggca attatcatag caaggaaatt gtcataaata aagagacagg
     6481 aatatataat ccagatgatt cacaattaaa gtggtaagga ggttatatta taaataattc
     6541 aaacaactta attaaaaata cagaagcaga gcaatcaata ttaggaagca ttttattaga
     6601 ctcaacaata gtagaagagt tagagcaaat gttaaatgaa gacatgtttt atcatgagga
     6661 acataaatac atatttaatg ctataaaaga aataaataag agaaatgaag taatagatat
     6721 aataactctt acagaagaat taaagagaaa aggtcatttg aataatattg gaggtataag
     6781 ctatattacc agtttgtcaa caatagtccc aacagtatca aatttcacga gttatgtcag
     6841 tatagttatt gaaaaatata aaaatagaaa tataattgat aaattcaata agttcaatct
     6901 tggagaaata gatgataata ctcttatata tcagttagaa aatgatataa ataaagccaa
     6961 tcagaaatta ataaaaaagg attcaagtat aacagccata tcagagagag cctttgaata
     7021 tataaatgat gaaatgaatg aaggtattaa gactggaata aagttcttag atgatactat
     7081 aggaggttta tatggaggtg aactaactac aatagcagcc aaaagtggaa gggggaaaac
     7141 agcattagct ttacaaatac ttagaaatgt aatatttcaa ggaaagaaag tactttttat
     7201 aagtggagaa atgagtgata tacagattct gtttagaaat atagcctcac taactggaat
     7261 atcagttgta aaaatgaaaa atagaaatct aaatgatgat gaaatagtca aatatataca
     7321 agctttaagc ataataaatc aagaaaataa cctttatata aatgactctg taaatacagt
     7381 atcagggata aagagagaaa taaagagtac taagcctagc ttagtcataa ttgattattt
     7441 gcaaattttg gattatgaag gtaaagaagt aggagaagct aaatttgctg acttatcaag
     7501 gcaaataaaa aagattactt tagattttaa tataccagtt attcagcttg ctcaattaaa
     7561 tgatgaattg aaggatgaaa gacctaaagg ggatagagta atgagaagct caaagcagat
     7621 atatatggat tctaattcag ttatatatat tcatagacct acagaaaaag aggctcttaa
     7681 ttattgcaag acaatgggaa ttgaggatat aagagagttg agacgaagag aaaaaggttt
     7741 tggtgctaat ttaagtgaga ttattgttgc caaaaataga gatggtgagt caggatttag
     7801 accatattgg tatataggaa atttgttttc ttttgaagaa tgtaagaaga tacaagagat
     7861 ataaaaaaga agcatgattt attaagtaat agataaatta tgtttcaaga agtattgaat
     7921 ggtaaatata taaaacttaa ttagatgtaa tagagattta aaatatattg agtttagatt
     7981 taaatgctta ttagaaaaag gtagttgcca tataaatata acaactacct ttataaatta
     8041 ggtacttaat gtacactaac ctagcaactt aagtatatat taagtacctg atgaaagtca
     8101 aggaggtact aaaatgtatg atgaaattat agaaatatat tttgattatt ggataaatga
     8161 acatatgtat agaagtattt tagaaaaaga tgagtattat attgaatgtt ggaaagaggt
     8221 taaaaaaatt aatgagttat tggaaataaa tttggaagat gataagaaat atctgttggt
     8281 tgaattaaat gatttgttta attcattaat agaatttgaa agagaactta gttataaatt
     8341 aggatttaaa acaggagcaa aatttataat agagttgttg aagtaattaa tttgtgtatc
     8401 tcaatagaat aattaaatag taaatcgaga ttattaataa gttgtactag aatattttaa
     8461 gtgaagtata tattaattat attgtttaaa ataaatatat ttaataaaga ggggataagt
     8521 atgccctctt ttatttttat gcaatcaatg acattgtagg atttacgtat tttgataaat
     8581 agcacaatat attttaataa taaatatatg atacaataat tttattaaaa tctaaaataa
     8641 tcaattgagg aggagaatat gataatacct aagttagagc ctaatgatag aaaaagagag
     8701 tttaataaat actcatcaga gcaaataaac aaagtagttt ataattattt atttaaaaat
     8761 aatatgagta atagaaagat agatgatgag atactagatt tatcatcaaa acaaagtaaa
     8821 ggttatcaat caatgggtat agtgcatcat ttaggaataa ataaaaatca taaaggtatt
     8881 tttacaggat acacaataga taaagcaatt gaagttctta aggaagatga gatacatttc
     8941 aagcagatta ttaatatact tttagaaatt agaaatataa atgagtatcc tataatagtt
     9001 gactcttggg agttattaga tgagaaaata gctttaaaaa caattgataa atctacatta
     9061 aaacataatg ggacaggagt tccaatagaa ataagatatt tttttgagat aaataattta
     9121 ggaaagaatg aaaagaaaaa tgtgaatttt aaatataatg ataattatta tagtgcagtt
     9181 atagagatgg ataaattaga gaatccaaga agtagaataa tttggaaatc taagtttaga
     9241 gatttactaa gaagtatatt ttctgagtat tatgagaaat tcttagaaaa taaaatagaa
     9301 ggaaaaacaa atttacctaa aataagattc ataaaacttg atagtaataa ttttaacata
     9361 gaattcattt ttactgctga aatagaagac gatgtattag aggagataag tcaagataat
     9421 cctttgaaca ttaatactat taatatagaa ttagaaactg aaattagaaa agaagggaaa
     9481 gttaaatatg tttatgggaa acagtatgag cgtaacccca gaaatagaat agaagcaata
     9541 aaatatcatg gcacaaagtg tattgtatgt gggtttgact tcgaaaaaac gtatggagat
     9601 agaggtaagg gatacataga aattcatcat attaaaccat taagttctgt tggagaagaa
     9661 agcaatataa atcccaaaac agatttggtg ccaatatgtt caaattgtca tagaatgatt
     9721 cacaggaaga aagataatgt tttgagtatt gatgatttga agcaaattat aaaagatagt
     9781 aaataatgct tatgtgtggc agagatgtat aaagtatata ttctaaacta gttaacaaca
     9841 aatactaata taaaaaatca aaaaatttat taggaaaagg tagttgctaa agtataacag
     9901 ctacttttat aaattaagta tttgaggtat attagtctaa ccgattaggt atatcttaag
     9961 ttattgatga aaattaatta agactaacat gtatgatgaa tccatagaaa tgtattttta
    10021 ttgttaaata aatgaacata catagaaata ttttagaaaa ttacaagtac tatattgaaa
    10081 gtttgaagga gaaccttgat ttaaggtaag tgtaaagttt ttagaaactt cactaaaata
    10141 atctaattaa gttatatatt tgttactttt aggaaaataa tggtaaaaat taaaggtttt
    10201 tataaatgaa tgtctaattt tataaataaa atggagtatt ttaactaaat atttgaggag
    10261 ataaaatgat attaaataaa atcgaaataa ttggagatat ggctcacttt aaaattccta
    10321 tgcatagcaa gatacaaaga acatatgaaa ttccaccaat atcaacagtt gtcggaattt
    10381 taaaaaatat atatggtgaa gaaatagatg attttatatt aggatatgtt atatatcatg
    10441 atggtacatt taaagattta cagactatat ataaagaaat caatccaaat gtaaaaacat
    10501 tgacagatag cgatagattt caaactgatg tatgtcccat tgaataccta attaatccta
    10561 aacttatcat atatactaac atagataaaa atatagaatt taatgagccg ctatgtttag
    10621 gtaagactaa ttgtttagcg aaggtattat ctttaagtga aattgaagta aatctaatag
    10681 ataaaaaagc tattggatac aatcaatata cagatattaa tattggagat ggaatgataa
    10741 aaagaataaa tacattgaca gagtataatg aaaaaaaagg ttattatgat atatattctt
    10801 caatggtaag agaaaatgat gagtttgaat atggtaaata ttatgatgaa gatgagcaac
    10861 agaatatttt tttatggaaa tggaaaaaag ggggagtaat taaatgcatt tagtaagaag
    10921 acatttgata ctcacagtgg ataatgaagt tgtattagat tacaattacc agtatgagct
    10981 aatgaaaagg atatatgaag ctatagaaat taatgataaa agaaaagcac taagtcttca
    11041 caatgaaggc tataaggtgg ataagaaagt atttaaatta tttaattaca caattatgtt
    11101 tgaaaatgct aagtatttaa aagagggaat acatttaaat ccccaaacaa aaatcaaatt
    11161 aattttaagt ggatatgatg atatattgaa taatattata aaaggtttta ttaaatgtaa
    11221 agtatttaaa ttgaataata tagagtttaa agtttcagat atagaagaag attcaaagaa
    11281 aaattttaat aatataacac tttataaagt aaggtcacct attgtagcaa gtttatatga
    11341 tttaaaaagt aggaaacaag tatacttaaa tccaatgcaa gaagagtttt ataaagcatt
    11401 acatgataat ttaggaaata agtataagct tatacataat aaagaatata ctggtgaact
    11461 atactttgat attgaggatg tattagcagt aaaaaagaaa tatataacta atataaaagg
    11521 aaaaggattt ataattggat atacagattt tgaaatattt gtacaagcta ataaagatat
    11581 gcaagaagta atttattatt gtggattagg tgaaaaaaat agtataggaa tggggttact
    11641 aacttatata acttctagga gggcttaata tggatagaag ttacaatgta tttgttgata
    11701 atggtctata tgtgttggct tattatctga acaaagatat taatgatata acttatcaag
    11761 atatagagaa tagtatagat ttaatgagtg ataaaattga agaatttgta agttgcgaaa
    11821 aatactctaa tttaaaaagt atgtgcttta gcaattctac gctaactcaa ccaaaaggaa
    11881 aagttacttt aaatgagaag ttaaaaggtt ttgttaaaaa tcaaggagat gaatattgta
    11941 gtttatgtgg tcaatataag gccaaagtca aaattgaaga taaagaatac aatataggga
    12001 gaagttatat gcctaatttg gttgcaaata cattttataa tttctccaat aatttgcaag
    12061 gtttgaatgt atgtccatat tgtttagtat tgactatgta ttcaattctg aattgtagag
    12121 taagtaggta tgctttttta tacaattcta catcaagcga atttatggaa gattatactt
    12181 tccatattca aaaagaaaat ttaactgatg tagagttagg agcaaagaaa gaaaaagaaa
    12241 agtattcaat tgtaaaatta ttagaaagtc tagtgtgtaa atataactta tttaatggtg
    12301 atatagagca atatatgttt aataactctg gtcaatctca ggatattcag gtaaatagaa
    12361 taaaaaataa atatgtgaat ttattaataa aattacagga aaaagcacta ctatcaaact
    12421 ttgaaaagtt acatttggat aggtatatct tgaatggtac tttagaaagc aattatctca
    12481 gagaagtata tagggtaaaa aaggaagaaa aaatggatga gaaagaacaa aagaattttg
    12541 atgaacttgt tattttttta aacagggagg taaatgtatt gaaaaaagtc aaaatagata
    12601 taataaaaaa attaacggat aaattaaaaa ctagtgactt agatatagac aaaatatata
    12661 aggagcttaa aatgttagga aaattttatg catatgagga ctggttagtt aacttaaatg
    12721 aaatgtatta tgaaaaaact ggagaaaaac tatttgaagc agaggattat attgagttgg
    12781 atgatataag aagctttaga cagattaaaa atttaatatt agtaagttta atttaataat
    12841 attatggagg gttaaaatgg aaaataaaaa ttcaaataga atgttaaata taggctatgc
    12901 aatagaagtt ttatgtgggc gattaaatgg aagtagtagt gaagtagaaa agaaaaataa
    12961 ttcatataag aattctactt atactaaaaa aatagggagc agaagtagaa tctcaagtca
    13021 atgtcaaaaa tacaatcaaa agctatttat ggaacaagtt tgtggttatg aacaaaatgt
    13081 taaaactaaa gatgggaaac aagtaaaatt ggttccaaac ccatttaaat atgcgacaga
    13141 tgatatattt gggtttatga tggcggaaaa attatctata gaagaagatg tatacaaaga
    13201 attatcagat gaagaaaaag aaatatataa aaaaaataaa aagaaatatg aagtaaatat
    13261 aactaaaaaa agaaagtcaa gatggcaaat gaactcttta ataaatgtat caaatagaaa
    13321 agttgaatgg gagtggaatg tttgtggaac tacaggagat agtatgccat atcaagttga
    13381 ggtatattca ggggtacata caaatatagc aaatcttaat ataggaaaga taggagaata
    13441 tgatatatct gatatagcaa gtgagcatag agattataca actttagaag ctaaatcaat
    13501 tggagttgaa aagttagata aagaagaaaa gtttaaaaga atagaagctc tcctaaaatc
    13561 actagaatac ttaagtgtag aaggtaataa gacaaatcat ctaactgata caaaacccaa
    13621 atttgttata ttgggagagt attcatgggg aaataatgta tttcagggta ttatcaaaga
    13681 aaatggctta gatgtagaga tgttaaaaga atctatagaa caaaatgaag aatttagaaa
    13741 aagcgatata tggattggaa taaataaatt ttatgatgat acatttaatg agatagaagt
    13801 taataatgaa aaggaacaaa taaagaagta tttagaagaa gagtttgaag agtatgattt
    13861 tatgaaaata tcaaatgtac atcaagcttt tgaaagttac ttaaaatatt taaaggagac
    13921 tttataaagg atatatcatg aataatttat atgaatataa agctaaatca gatgggacaa
    13981 gtatagataa gcataatgaa gatatgaatt acataatgaa tcaattagtt agcatatata
    14041 atatatctaa tgatatgaaa aatgatttat atgaggcaat tgtatgtcat gatgtaggga
    14101 aagtagtaaa aagctttcaa gataatattg aaagtagcac aagaagagtt agacatgagt
    14161 tattatcagc aagtattaag tatttaaata gaggtcagaa gttagctata ttaacccacc
    14221 ataaagatat aaagtttcta gagggtcaaa taacaaattc tatttatgaa attgaattaa
    14281 aggaattaaa ggaactatta gatattgaag tagaagatat aagaccattt ataaaggaaa
    14341 tatcaagagc aagaaatgat gatactagaa aacttgaaaa tatattatta aaaggttact
    14401 tgcaatattg tgaccatata gcttctgcgg gaattaaaag tatagataaa ggatttaatt
    14461 ctattaaaga ttttagtata ccaaagggtg taagttataa ttcaatacaa gaaaaagttt
    14521 taaatatgga taaaaatgaa gatataatga taatggctat gacaggtctt ggaaaaacag
    14581 caacatcatt gtattggagt aacctagttc aaaatgaata taaaagtaag agagtgtatt
    14641 atatacttcc ttatacagca agcataaatt ctttatatga aaatttatat aatagaaata
    14701 taagcgtaag tatgttacac agtaaagctc catatttttt gaataaaatt agtgatgaag
    14761 aagatataaa acaactttac cagatataca aaaagagtgt aaaacaaatt aatgtatgta
    14821 ctatatttca attagtaaag tcagtatttt cttgtaagag atttgaaatg ctactatcac
    14881 aattaaaaga tagtatattt atagttgatg aaatacattg ttttgataca agagtattgt
    14941 gttatacatt ggaattttta aaatatttaa aagaaaactt aaatgtaaaa atatgtataa
    15001 tgagtgcaag tataccaact tgcttaaaaa agttaataca ggaaagattg caaataagta
    15061 caattattga accagatagt aaagatttaa tatttagaca tagattgaat agattaagtg
    15121 aaggaatact tgatagcata cataaaatag aaaatgattt aatgcaagga aaaagagtat
    15181 taatatgtgt taattcagta gatttatctc aagagttata tggaaggctt caaaaatata
    15241 atcctaaatt aatacatggt cgatttaata ctagagatag agagcaggca gaaaaaggga
    15301 tagaagaaag cttcttattg ataggtactc aaagtataga ggtaagttta gatataagtt
    15361 atgatgttct ttatacagag ttagctcctt tagattcatt gttacaaagg tttggtagag
    15421 taaatagaag gggagataat gctgagttga gcaatattta tatatatgat tatgatagaa
    15481 agcttaatat atatgatgaa gatataataa ataggactga tatagtaata tcagatataa
    15541 tacataatga taattctata gttgatgaga gtaaagttcc atcttattta gataatgtct
    15601 atttagatat agatatggat aaatataata aacataaagt tgctttagag attataatga
    15661 aaaatatgag gttaggggta tggaataaat ctgctaataa tgatatgtta aataatgata
    15721 ctattagtgt tgtgcctgaa tgtcttttag aagagtacaa gttgtatatt gataaaaaaa
    15781 tgtatttaga agcgagttca ttatttgtaa atgtatctgt aaaaagatac aattataata
    15841 aaaagatgtt ttatgaatgt ggagatatag ttgttacaag ttatagttat aatgataaac
    15901 taggtctaat atttgattgt taagagaatt atagtataac taattaaaat attttattga
    15961 gaatttgtta taatttatat cgactacaaa ttttatatgt tacaatagaa taattttgca
    16021 gtgagcgaaa aatatgtgta aatggctgtg agttagtgat ggcaaggttt ataactgaat
    16081 ttttgttttt agctaaaaac actactggtg cctcactgca aattaactat tttaataacc
    16141 ctacaagtgt tgtaattact atagttttat ttggggttta aattacacta agttagttat
    16201 aaatattccg gaaagtactt ttgctatttg attcattcta cccgtttaaa ttacactaag
    16261 ttagttataa atttcactaa ttttagttac aactattaca agtccagccg tttaaattac
    16321 actaagttag ttataaatta tgtgtaatgt attttaatgt tttctagcaa caatgtttaa
    16381 attacactaa attagttata aattttataa attctatgaa taattttaaa gagaggtttc
    16441 gtttaaatta cactaagtta gttataaatt gtacatttga catagaagct tcaaaatcca
    16501 tcccagttta aattacacta agttagttat aaatgtaaca gcacgaatga tggcacagac
    16561 ctatctgcaa tgtttaaatt acactaagtt agttataaat agaagtacac ttacaagtaa
    16621 gataaaagac ccaggtacag tttaaattac actaagttag ttataaattc tgtagctata
    16681 tgtggaagta attttagtag agaggtttaa attacactaa gttagttata aattattgaa
    16741 ggggtgagag ttgtgactag gcactacaag tttagattac actaaattag ttgtaaattt
    16801 acttattcat taagaaactt aactgagagt ttgatttata ttattgaatt agcaagagtt
    16861 ttagaaagta gaaaattata ccccaataat accccaacca caaaaataaa taataaaaaa
    16921 tatccaaaaa aattagtata acatcataac taaatagcta tatacctaca cttacgaaaa
    16981 ccaacaaaac aatcaaataa ccacttgtag actcccctcg tctccaccaa ttttgaaaat
    17041 gaaaataata gaataaaagg aattacttat gtttggacta agtaattcct tttattttgc
    17101 ttaaaagcac acaattagca cacaactaga aggttatata aataaaaagg ctgtcgcact
    17161 agaaaatagt gctacgctct tttttgtata aaaaaataaa tcccaagttc aaaagaacct
    17221 gggattcttg tataataact gtatgctaaa acaaaatact acacaaattg attatactac
    17281 ttatggtgat aattatcaat ttaagctacc actaaatatt gattacatga ttccaaaaga
    17341 tgattctgta aggttattaa gttaaattat tgaggagatg aatttagaaa aattatataa
    17401 gacttattct cgaattagat gaaatagagt aatccataga caatttttga aagttataat
    17461 atatacagat atgaactgta tttattcttc gagagaaata gaaagaacat gcaaaagaga
    17521 tattaatttt atgtaccttc taggcgaaac ctccactcca gaccattcaa caattgcaaa
    17581 atttcgcagt atacattttt aactaatagg caaaagcttg attgcacagt ttacaaactt
    17641 tcttggagat aataatgaaa tttcaaaaga cacaatattt attgatggta caaaaataga
    17701 gtcatctgct aatagatata cttttgtttg gaaaaaagct gtaaataaaa atatggctaa
    17761 aatggtgaat aatatatctg acttcatatt gaaatgtgag attgatgttg gcattaaaat
    17821 agtttatgaa gataaaatta aaatctatca tttaaagaaa ctaaaaaaaa tatcaaaaga
    17881 agcaaatgta gcatttattc atggaaaagg aaatagaaaa tctactaaaa accttcatat
    17941 aatgggtgaa cgaaatagtt gttctaaaac agataacgat gaaactttta tgagaatgaa
    18001 agaagatcat atgaaaaatg ggcaactgaa accagcatat aatatacaat ttggtgtaga
    18061 ttaagaatat atagtttggc taaattcagg aaatcaaact gctgatacta caacattaat
    18121 accattttta aaatctatag aggaatatac taagtttaaa tatcctaaag taatagctga
    18181 tggtggatat gaaagtgaat aaaactatgc atttttagag cataataatc agttatcttt
    18241 cataaaacct gcaaattatg aaattattaa aactagaaaa tataaaactt atattggatg
    18301 tattgacaat atggattatg aagaagaaaa taattattat atttgtacaa atgagaaaaa
    18361 actatttgct aataaaataa taaataggaa atcaaaaaca ggatataaaa gtgaaataac
    18421 atgttacatt tgtgaagagt gtagtaattg tcaatataaa agcaattgta ttaagagaag
    18481 taatagtaag gttcctctta aaaataggac taaaaactta caagtatcta agtcgtttca
    18541 tgaaaaaaga aaagaaaact taaagagaat tatgagcctc gagggggatg aacttagagt
    18601 gaatagaagt atacaagcag aaggagcttt cgctcaagta aaataaaata tgaaacttac
    18661 aagattttta agccaaggta aagaaaatgt tttataagga tatattattg tggcgatagc
    18721 ttataacgtt tctagactac acaataaaat agtaaaaaat aaacgtggaa tatatcttta
    18781 tcatttaaaa aaatattcta aaattattat ttataatcaa atattatctg aaaaaatggt
    18841 atttttatat ctttatttgt catgcataat tttacttaga acagaaactt tatataaaac
    18901 aattgaaaat atacctatta agataattag aaaagagctc tcacagaaca aatttaattt
    18961 gctcatgcaa cagccccata ttgatataca tttaggttgt ataattatac atttcaatat
    19021 tacatgttca ttaggataat agttagtaaa tgatatgatt gagagaatag atataataaa
    19081 ctaatctagt cctgtattgc aatgtttatt aaagaatata tttttaaaga tccaagtact
    19141 gcatctcttc cagaattgag ttggttaata gttttttgat gctatgcaat ttataataaa
    19201 aagtataatg atttagttga tgaatcatca gaatttatac cctcaaagat taatcagatt
    19261 gcaataaaaa taatgatata ttctatagtt ataatatcta ttttctttgc aacccctcat
    19321 acaatgaata taaatatatc aaacttggat atgggtataa ttctcatttc agttttattt
    19381 atacagtttt tatctaaatt aattttgtat gtatattttg ataaaaggga gatatgtaac
    19441 taagtggtaa tattggtgaa aacaaaaatt aaagaatata gaaaaaaact ccttatgaca
    19501 caaaatgaac ttgctaaatc agttagggta aggtgtgaga ctattgtaca cttggaaaat
    19561 ataatccttc tttaaagtta gtgatagata ttgctaaagt attcaatact acagtagaaa
    19621 atttgtctga atttattgaa gaaggatagt ggatagatta taaatagtta taagatggtt
    19681 aatgaaatat agttacttaa ttattaagtg tgttagaaat atttatatgt aacacaatgt
    19741 gtagttaaga ttaccttatc tcatctaaga ctttaatttt tcttcccata aagagggtat
    19801 atcaattttc tagtgtggta gcccattttt tattgattta aatttataat atatattaat
    19861 agatgatact taaaaatttt ataaaatatc taagttgtca taaataaaac gatatatcta
    19921 accgaatatg gtaaaatttg ttataatata gataagttag agtaaaaata attttaatgg
    19981 gggagaaaat gcagtcagta atatgtaaaa tttttgataa taaattacta attagtaatg
    20041 atttaagtga gcaggatgat gtattatatg aggtaatgga taaatttata gatgatattg
    20101 aatatatttc aaaattagga gatgagcaaa ttaaaagtat gcttatggag gtaaataaat
    20161 ttgaagaaga ttcaactgaa tttgaactat atatgaaaaa cttatgctat ttaaagcaat
    20221 catattttat agatactaat gataaaagtg aaataaattc tatagatata gatatattaa
    20281 aaaatgttaa aatagaggac ttagttgata taactaatat taaattagaa cgaaatttaa
    20341 atataaatat agaacatata tctaaagctg taaataaaat atatgaagaa aatgaaaagt
    20401 ggaaagagta taatatacaa aatactatgt ctaaatatat aggtatacct gaatcagaag
    20461 ttatatttat atcaaatttg agtcagtatg tttcattttt atctgaacta gataacgcag
    20521 gcgactatgt atctagaggg caaaaagatt gtatgtatga attattacca tcattacata
    20581 gaaaatataa aaaagactat ggtatacatt cagatgaata cgaggaagca ttcaaacaaa
    20641 aaataattta ttatgataaa gaaataaaaa atagatcgag tgaagaatta agagctgaag
    20701 ggcaacattt tggactacca acagaatatt tagatttcac agaagctcat ttaatatcat
    20761 tattatttgc aatagaagac tataaatata caaaacagca ttcaattgtg tactttatag
    20821 actctatttc ttataatact gacactataa aaagatctga gaagttagta aattattcag
    20881 atacatctgc aatagataat tgtagtaaat tttcatcaag atcatttttt ataaaggtag
    20941 ggaattcaaa tgaaagaata cattttcaaa aaggatgttt cctaaaagta tcatcagaag
    21001 ataaaggcga atttaaggaa aaattaaaaa agtattgtaa agtagtagtt attaataaag
    21061 atgctaaaaa agaaatatta aaagaattat ttaatttagg tattacattt gaaaatattt
    21121 atccagataa agataatgta gttaaatcta taaaattcta ttatgatgag atagtagggg
    21181 gaaatttata atgagagaac aatttaaaaa ttctataaaa aatattaaat caaacagacc
    21241 tcttgtatca gtccaagata ttgtgaattt tattgataaa ggagaaaagt acttgaaatt
    21301 atcaatagat gaaatagatg aaatggaaat tttacaagga atagttatga gtcctgatta
    21361 tcaacgtaca tataaatcat caaacaagga agaaagttct ataattgaat ctcttttaat
    21421 tggaatacca atacctgaaa tatttcttgt aaaaataggt gtaaatgata tgcaaataag
    21481 acatgtaatg gatgggcagc atcgtttgaa tgctatatat aggtatgtta agaataaata
    21541 tccacttaaa aatttagata tattaggcaa tgacccacta tatgataata aaaggttttc
    21601 agaattagag aaaaaagata aaattaaaat attaggaagt catttatcaa ttctagaatt
    21661 tgatggtttt gataatccag aaatagaaat agaattattt aaaagatata acagaaatac
    21721 aaaaccatta gagatgcaag aaatcgagat ggcaacatat ttttcagaaa ctagtaagta
    21781 tataagtaaa tttataaata ctctaataaa agataaatat aaggatgaaa tagataaatc
    21841 agaatataaa tatgctaatt acaaatcaga tttacttaga atatataaca taactaaatc
    21901 tcgtaatgat aaacaaaaaa atcatcaaga aatatgtata atatttagta ttttagaaaa
    21961 tgggctacaa gaaaatatca gagatggagt tacagcctct aagaaatttt tagagtataa
    22021 gtcaattcag tataaaaata atgaaagtga aaaattagag ttattaaaag ataagtttga
    22081 tgagtttaat aggatgatac tgaaaatatc tgagaagata gaatatccat tctcaacaag
    22141 tttaatatct gatgataagc aaagacaaag taaatacttg ataggagtat ctattgtgtt
    22201 ggcttctatt tgttattatt atgatataga cttggaaaat aattatttaa tacaagatat
    22261 taaaaaaata atatcttgtt cacccatagc agatatagaa tataaggcgt cttcaactaa
    22321 tatgaaaaat atgattctat acttatttat aaagaataaa atacaagata ataaatttga
    22381 atcattaaat ttaaaaagag ataaaattaa agaaataaat actttagtaa tgaataatga
    22441 tggttattta aatgaacaga caaggctatt agaaatatat taacaagtta taaaataaat
    22501 catggtaatg taaaataatt tgtgctttta aggttaataa aaatcttctt tatggcaatt
    22561 atttaaatat aatcaaatgt cgtaaaggag attttttatg aacgctttaa gaaaacaatt
    22621 agccaaagaa attatatcag atatctaaaa aagagcatgt caaataaaac cctaggtatt
    22681 taaatttttt tagagctact taaaataaat tatgctatct ctgtatttaa atatttccta
    22741 aattcaagtg gagacataga atttaatctc ttttgatatc taaaattatt atagtaatat
    22801 atataatcct ctattgtttt gactaattgt tcgtatgtat caagttttct taaatagtac
    22861 atttccactt ttaaaattct ccaaaatcct tccataggag cattatcaat acaacgagca
    22921 actcttacat actttgtatc atgccttgtg ttaaaagttt tgatttaaat accttactag
    22981 tatactggaa acttttatca ttatgaaaaa taggttttgc atttggatat agctctattg
    23041 ctttatcaaa agtttctaac accaagttat tgttatttga ctttccaata acatatgaaa
    23101 ctatacttct atcgtctaaa tctagaattg cgcttaaata ggccttcatc ccattggtta
    23161 atttaaattc agttacacct gttaaccatt tttgagaggt tttatctgca ctaaattttc
    23221 tacttaatac attttccgcg atcacttctg gcgtacattt aacataacta aatttctttt
    23281 ttctgcaaac agaacttaag cctacggctc tcattagtct gtacactctc tacaatcttt
    23341 aacaatttca actctttcat ctaatgctgt ttttctagtc tccataaaga tttctccctt
    23401 cgaggtttct tttatctact tatgaggatt atactcttta ccccaagctt ttagtgaata
    23461 aactgatctc attttatatt tttttgagat tgtgtcataa gatccttcac catttagata
    23521 agattttata gcattcaatt taaattcttc acaatagcat aatttacaac tttgttttat
    23581 taaaccatca tttccaaaag cttcataatt tcgcatccac tctctgaatg tacgtttatc
    23641 caatatttaa ttcttccatt atttttatta ctgtattctc accttttaaa tatgattcaa
    23701 ttgcttcgat ttttttgctc tgaagatatc ttagttttag atagtcttac atattttaac
    23761 tgtctcctaa gatgagtata attcattcat catcagttct atagaataca cctagttcct
    23821 acttactgta ggaactagga ctatatgaaa cccattcata gattgtagtt tcttctgttg
    23881 cattatagcg cagaatagta aattcatgtg aaattttatt ttgaatttac tatttatata
    23941 taaatgaaga gtggtcttat tttcaattaa aatttggaac aattttgggt tgacatctac
    24001 aattaaggat ttaagttttt gttatactaa taaataaaaa cagagattca aaatatataa
    24061 ataatactta aactagtgct gatagatttt ctttaagttc tagttttttt attgtcatta
    24121 tctaatatgt actattttat gtacagtcaa tataatctga aattgtaaaa tggtgtataa
    24181 tatagttata agtaaagaag ggatttaaac atgtatgatt acaaaaataa aaaagactta
    24241 tatatacctt aaggaaatac aaaaaatata caaaatcaat gagcataaag aactagtaca
    24301 cataataaaa aaactaataa atgataataa aataaaacca ataaaaacct cagatctaac
    24361 acctcagcat gagcaattat atacaaagta tagaataata aaacaagaaa cagaagaaga
    24421 taaaaaaata ttagaagaaa taaattacaa aatatgtgat aaattaagta tagattatta
    24481 tagaaaaaac ttagtacact ataaaaataa tagagttgca ataatggatt taaatagtta
    24541 tcttattaaa aaatctgata atctatcaaa gaaaatatcg attaacgaaa gatcttatga
    24601 aatatttaag gatgagaaat tcataagtag taaagtagga aaagaagtac taaaaaactt
    24661 aaaaatagat ctaataaagg atttaaacgt atataagaca ccagagccat tcatatattt
    24721 atcaataaat cgtatatctc cacaaaaaat attaattata gaaaataaag acacttacat
    24781 aacaataaca aaaatgctat tagaaggaaa agaaatactt aaaaataaaa tagatactgt
    24841 tatatacgga gaaggaaaaa aaataatcaa cagtattaaa ggattaaagg aagatataac
    24901 aattgaatat cttacaaata aagaaaatga atttttatat tggggagata tagataaaac
    24961 tggattatca atatacactt ccctaaaaga taccaatgaa attaataata taagattatt
    25021 tgaagttgct tatgagcata tgatagataa aacaaaggaa aataccttga ggaatatccc
    25081 caaaaatcaa aaagataatt ttgaatgtgg attagaaaat attaataacc taaacttaaa
    25141 agaaagtatt aaaaatattt tagtacaagg aaaatacata cctcaagaag ctctaaatag
    25201 attcgacttg gaggaatata actaatggaa atgattaata ttttagaagg ctacaataag
    25261 agagtaaact gtataggatc ttattatctt attgcaactc ttgctaacaa tagaaataaa
    25321 tttaaagaat ttgacaacat tcaattttac aatttattaa ttcaagtttt gtgttatata
    25381 tttgatagat ctcttagaag aaagaactgt ttaagagatg atattaagga ttttattgaa
    25441 gaaataaatc gtatggatta taaaataatg ctttcagagg atgacttaaa ggatttagca
    25501 aactatataa ttaatggatt aaccaatagt ggtaaagtat atctatttac ctactattct
    25561 ttagaacaag aaaaacatat agatgaaagt attaaaatta tagaagataa aaatgtaaaa
    25621 ataaataatc aggaaagact atcctactcc ttaacaacag agggttatag attgttgtta
    25681 tcaacaaaag aatatgatga actattccaa attcaaatat cacaaatgat tgcaaagtta
    25741 aggatagaaa agggtgatta tcaaggcgca aaggaagata tttacgatat aataaatacc
    25801 ttagagattc atcaacagaa aattgataat tatataaaaa taataaaagt aaatatacta
    25861 tatataaaag aagaaaaata ttcaacagta atagatacaa ctgtagaaac acttttagaa
    25921 gaaatggaca aatatgaaga attaaaaaaa gaagtaaaac tattaatcaa agataaagag
    25981 gactacttgg aaaaggatga ggatatagac aaagaaaaga taaaaaatat aagaactcaa
    26041 ctattacaaa tgaaagaatt aatagatgga atagttttag caaagtctac agccacaaat
    26101 ttgttatcta gagttcaaga ttttagagaa gaatttaaag aaatacttaa aaaactatta
    26161 acaactccag taattaaaag attcaatttc aaggaagaaa tcttagatag acttgaaaaa
    26221 gacagtgatt ttttattgat aataagagaa ttatatatat ctttatttaa agtagagttg
    26281 ccaaatcgat ttaatattgg agtggcatat caagaacaga aactattaaa tactagagaa
    26341 attgaaaatg aaatagttaa taaagacgag tt
//
LOCUS       DACTGW010000031        26351 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.031, whole genome shotgun sequence.
ACCESSION   DACTGW010000031 DACTGW010000000
VERSION     DACTGW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26351
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.031"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            30..485
                     /locus_tag="I9573_001028"
     CDS             30..485
                     /locus_tag="I9573_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795656.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(710..970)
                     /locus_tag="I9573_001029"
     CDS             complement(710..970)
                     /locus_tag="I9573_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795657.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(1233..1586)
                     /locus_tag="I9573_001030"
     CDS             complement(1233..1586)
                     /locus_tag="I9573_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795658.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQAKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGRKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(1603..6780)
                     /locus_tag="I9573_001031"
     CDS             complement(1603..6780)
                     /locus_tag="I9573_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HAT4795659.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIDDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVVTYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFAKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSNVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDSNMFIVMNDGTLYACGLNNYGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGNSTFVLKNNGTLYSCGLNSNGQLGLRDEVNRNIFTKI
                     EIENVKDFCVGSNYVIALNHSKEVYGWGNNPYNNIEKTSNYPYKQGISNIEKIAAYDY
                     SVYMINSEGKLYVSGYNYNYQLGKGNNSNQSKALVSQCRTNSTSSTSNGLRTLPKITN
                     VFPFYDGCAIIDEGGYVYLTGYHGYLRTLNSSPSISDYSRYGTFIEATNSNHNTYFIQ
                     ETDFSGIEKVIGMSNNILFFKKGSSYITGYPKTFGSTITGHRSYTSINSESSNLGSNF
                     IIYHSNSKLYGKGIANSGQFGNSTNIDGTSNYDTGLKDIKDIIVKGNTVVVVDKNNNI
                     YVTGMNQNNKLGIGEYNNEPVKKFTNITEQSNSFIFMDDIKEITTSRNTMFIVKNDGT
                     AYATGNNSSGQLGLGDTINRNKFTQINLDNIKKISTSIDGNTTFAIRNDGTLYSTGLN
                     TKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDDNTLYSCGENTHGQLGLGS
                     ESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCCGYNNNSQLGMGNTTDQYS
                     FIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGLNSNSNQSSFSEIPISNVV
                     KVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIKKIASKAKDIGMNYRCGHY
                     VSDNGDLYGTGFNNNGQLGVGDVTKRDTFIKTNTRVKKILPLEYANIAIKDTNDIYIC
                     GLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIKNRHNFILLNNKIVIPTTK
                     DIDYGLVLGNLYKGDLYTELPYEDIKEVSISKTHIIILLNDGTMYGCGTNYHGELLQD
                     LSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIGKNSEYQLGIGHNNPVTEL
                     QRITTISSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALGLGSDSENTIIKFFTKALT
                     DIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEFLKEFTIVPISEDVNTVKD
                     VLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNKDISRIEMLINGESVKSVS
                     DLTTEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFIFKESTGNSIIKDSYVMIG
                     NRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINKTKVQVKINKSDLFKDMKL
                     VEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(6802..7788)
                     /locus_tag="I9573_001032"
     CDS             complement(6802..7788)
                     /locus_tag="I9573_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAT4795660.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGDTDILTTDSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLANGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLSKYSYEIR"
     gene            complement(7789..8487)
                     /locus_tag="I9573_001033"
     CDS             complement(7789..8487)
                     /locus_tag="I9573_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HAT4795661.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSEMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(8484..9536)
                     /locus_tag="I9573_001034"
     CDS             complement(8484..9536)
                     /locus_tag="I9573_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAT4795662.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGENNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(9529..9957)
                     /locus_tag="I9573_001035"
     CDS             complement(9529..9957)
                     /locus_tag="I9573_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HAT4795663.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(9957..10283)
                     /locus_tag="I9573_001036"
     CDS             complement(9957..10283)
                     /locus_tag="I9573_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HAT4795664.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(10299..11828)
                     /locus_tag="I9573_001037"
     CDS             complement(10299..11828)
                     /locus_tag="I9573_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HAT4795665.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(12110..12532)
                     /locus_tag="I9573_001038"
     CDS             complement(12110..12532)
                     /locus_tag="I9573_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795666.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEVATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(12532..14985)
                     /locus_tag="I9573_001039"
     CDS             complement(12532..14985)
                     /locus_tag="I9573_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795667.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMAKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKRVEKRPYQGSINLKDMVSSAMARILPKLMLFKNT
                     FWSGVIAIKDMASSIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKTSQKDVDKYYDSLVNMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(15179..15625)
                     /locus_tag="I9573_001040"
     CDS             complement(15179..15625)
                     /locus_tag="I9573_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAT4795668.1"
                     /translation="MNENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(15739..16167)
                     /locus_tag="I9573_001041"
     CDS             complement(15739..16167)
                     /locus_tag="I9573_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HAT4795669.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(16181..17245)
                     /locus_tag="I9573_001042"
     CDS             complement(16181..17245)
                     /locus_tag="I9573_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HAT4795670.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTEEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(17250..17693)
                     /locus_tag="I9573_001043"
     CDS             complement(17250..17693)
                     /locus_tag="I9573_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795671.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTITLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(17942..18382)
                     /locus_tag="I9573_001044"
     CDS             complement(17942..18382)
                     /locus_tag="I9573_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795672.1"
                     /translation="MENKKDILFKETDERLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(18487..18684)
                     /locus_tag="I9573_001045"
     CDS             complement(18487..18684)
                     /locus_tag="I9573_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4795673.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            18853..19173
                     /locus_tag="I9573_001046"
     CDS             18853..19173
                     /locus_tag="I9573_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4795674.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            19255..19761
                     /locus_tag="I9573_001047"
     CDS             19255..19761
                     /locus_tag="I9573_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795675.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            20462..21217
                     /locus_tag="I9573_001048"
     CDS             20462..21217
                     /locus_tag="I9573_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAT4795676.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(21360..21788)
                     /locus_tag="I9573_001049"
     CDS             complement(21360..21788)
                     /locus_tag="I9573_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAT4795677.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(22209..22409)
                     /locus_tag="I9573_001050"
     CDS             complement(22209..22409)
                     /locus_tag="I9573_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAT4795678.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(22592..23746)
                     /locus_tag="I9573_001051"
     CDS             complement(22592..23746)
                     /locus_tag="I9573_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HAT4795679.1"
                     /translation="MKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNMEEKVM
                     ESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYENEVKK
                     MKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQKDKENA
                     FRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSEDIRNYIGQQDGVELQLT
                     DEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGRFKKNS
                     IWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFVGYEKK
                     GRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            23997..24818
                     /locus_tag="I9573_001052"
     CDS             23997..24818
                     /locus_tag="I9573_001052"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HAT4795680.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(24894..26282)
                     /locus_tag="I9573_001053"
     CDS             complement(24894..26282)
                     /locus_tag="I9573_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HAT4795681.1"
                     /translation="MLRSKKSNNKKVEKLRTIFIKYLSLFFVMTISFALLLMLSFSVL
                     LSSGVILPANYSEKQFDKYKEKIISSEKAIEDIIPNLYEYGVYTLDGNLISGTFNKKE
                     SKQVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKLVSEYSSPVLRAYLPKPEMLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNILF
                     SMDKMKLALKNSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAINMSEFIKDVLLQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVSNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
BASE COUNT         8269 a         4562 c         2841 g        10679 t
ORIGIN      
        1 taaatattta ttaaaattag gaggataaaa tgggcttaag agacaaattc gcgcaatcat
       61 ttgctagatc aaaaactatg agtggccctg agaaaaaggc taatgaaata atgggtaaat
      121 tgcttcttaa gaaagctata ttacctatcg ttttaatgtt cgtgataata atcgctggag
      181 ctatgcttaa aatcaattct tgggttacac ttggcataaa tttagttatt gctgttggtg
      241 ctttcttcta cataagaaat tctagtaaaa agtatcagaa ctttaaacct tatgttggca
      301 atttgattag tttagagaaa aaaggtaaaa aagagtacgt tgctattata aaacaaggta
      361 agttaccagt taagttacaa atagcttatg gtggtgaaga ccttgaacat gttaagaaaa
      421 atcaaatggt tcagataagt tataatccag atgctaaaat agctattttg gttaatagac
      481 aataatttaa taaaaaaaag gagttgtctt aaaattgatt ttttctaatc aaatttaagc
      541 ttaactcctt tttgaatttt tatatgttat ttttatatat tatttttatt acttttttta
      601 attaatattt taaaatataa aatccttctc aataaaatag ggcactatct acaatactac
      661 tttatctttt cctaataata tataatctgg tgctactgct actttcatgt tatacaccgt
      721 tttgtaacat accttttata tcagtaacat cttccttaat ttcctctaca tctgttttca
      781 ttattcccat ttgaacaagt atatttttgt ttatttcttg ttgttgtgta gataattcta
      841 taaaattttc tacagttttt ttatacatgt ctctatcttc ttttttctcc tgcatagtat
      901 ttttaaataa tagagcacat agtataccta ttgcccccag actactcaat tcggttatta
      961 atttatccat tatagcctcc tattgtctaa attttttata cttatagctt taatttttgt
     1021 tatattctta catcatgcaa tattatattt tcaggattat caatttaatc ttctgtactt
     1081 taaattgtag gtttaattta tagattgaat aaataataaa tatatataca atttctaata
     1141 taacaaaaaa taagtacaag gcttacttat taaagttaag accttgtact ttacataaaa
     1201 aaaataacta atacatagtt attctaaatt tactattcaa ctggtatttc taattcttta
     1261 catctaattc taacttgctc tctaattact gctggtactt cttctattgt ttttctgcct
     1321 cttaaaatta aagctacata tacttctacc attccactct cacctccaga ttttcttaca
     1381 ctaaaagtgc ttatattact tgcttcattg acagaacttt gacttaacaa catctcatat
     1441 atttctgcat ttgctataaa attatttgtt atatcatcat cttgctttgc ttgacctata
     1501 cttatgtctg aaatgtcttt atactgcatt tctacttttc taccttgttt gatttgttct
     1561 attttatttt tatcgaattt aaacaatttt tacacctcca ttttaagctt gtggctcgtt
     1621 gttttcagca tcatatctaa aaatcataga tggacgtttt atagctgtcc atttatctcc
     1681 tttttctact atgattttag gctgagcact ttttatgttg gcttcttcta attcatagat
     1741 ttcttgataa cttgagtctg attttttgat ttcaactagt ttcatgtctt tgaataagtc
     1801 agatttattt atttttactt gaactttagt tttatttatt aattgatata tagggtctcc
     1861 aagatttaaa tcttcttcaa gtcctctatc tagtgtaatt gtaatatctt gttcattaga
     1921 tgttgtatta actaccttgt acattctatt acctatcata acataagaat ctttaattat
     1981 agaatttcca gtactctctt taaaaataaa taaagatgca tataaatcat cacctttaca
     2041 ataagctcta aatagtatct tattctctcc tattttaatt ttatctggtg gtacttcaaa
     2101 ggatattttt tcagtagtta aatcacttac agattttaca ctttcaccat ttataagcat
     2161 ttctattcta cttatatctt tattagggtc ttgtattttt atcttaattg atgaaattga
     2221 tttttcagta atttctatag cagcatttgt cgttcctact tctgatataa tatataatgt
     2281 attgtctgtt gcaagtacat cctttactgt atttacatct tcagaaatag gtactatagt
     2341 aaattctttt aaaaattcta ctggttgttc aattttatat acatccctat tttttccagt
     2401 aacccatact gaattatcat ttttaagtac taatatatgg tcacttccat aagattttat
     2461 ttctcttatg tctgttagtg cttttgtaaa gaacttaatt atagtatttt cactatcgct
     2521 tcctaatcct aaagctccct tatcattata tccttgtaca aataattcat tacctgtagt
     2581 tactactaat gtatagtttt taccacaatg tacttcttta cagctagata tagttgtaat
     2641 tctttgtaat tcagtaactg gattattgtg acctatacct aattgatatt cggaattttt
     2701 accaatagac caaagactat catcagattt tataaaataa gtaaagttat ctccacatga
     2761 aacatgcttt acatctgata cattaatctg cacaaattca tccacttgat ttatagacaa
     2821 gtcttgcaat aattctccat ggtagtttgt accacatcca tacattgttc catcattaag
     2881 taatataata atatgagtct tagaaataga tacttctttt atatcttcat atggaagctc
     2941 agtataaagg tctcctttgt ataaatttcc taatactaaa ccataatcta tgtcttttgt
     3001 ggtaggtatc actatcttat tgtttagaag tataaagtta tgtctgtttt taatagatgt
     3061 caaatcaccc attacaaaat tcatatgctt ataattaaat attctattat cattatttct
     3121 actatcgtat ctatttccaa cacctaattg tccatagtta tttaatccac aaatatatat
     3181 atcattagta tcttttattg ctatatttgc atattctaaa ggaagtattt tctttactct
     3241 tgtattggtt tttataaatg tatctctttt tgttacatca ccaacaccta attgtccatt
     3301 attattaaaa cctgtaccat ataggtctcc attatcactt acataatgtc cacatctata
     3361 attcattcca atatcctttg cctttgatgc tattttttta atcttctctg gagtctcact
     3421 tcctgtacct gaaccattac tacatttgcc tgcagtatat acatcccctt cacttgtaag
     3481 taaaagtact gcattatttc tgtttggagc tacttttact acatttgaaa ttggaatttc
     3541 agaaaatgaa ctttgattgc tattactatt tagacctaag caataatcag tatttaaacc
     3601 tgtactatat gcagtattat agattgttat tatataggta tttatttcat ttggtataac
     3661 ttcttttaca ttttccatac actttataaa actatactgg tcagtagtat ttcccatacc
     3721 tagttgtgaa ttattattgt atccacaaca atatgcaatg tttgtgtcct ttacaataaa
     3781 tgttgttgta tctgagcagt acacatctct tacattagtt atattgttta cagtaaatgt
     3841 caatacgtct ggatggttgc tttcgcttcc taagcccagt tgcccatgag tgttttctcc
     3901 acatgaatat aatgtgttat catctttgat tgcatgcgag tgagtagtcc ctaaaacaac
     3961 atctcttaca ttttggatgt ttactttggt aaatgtattt ctatttacta tatcacctaa
     4021 tcccagttgt cctttggtat ttaatcctgt ggagtatagt gttccatcat ttctaattgc
     4081 aaatgttgtg ttaccatcta tacttgttga tattttcttt atattatcaa ggtttatctg
     4141 agtgaactta tttctattta ttgtgtcacc taatcctaat tgtccagaac tattatttcc
     4201 tgtggcatag gctgttccat cattttttac tataaacatt gtatttcttg atgttgtaat
     4261 ttcttttata tcatccataa atataaatga gtttgattgt tcagttatat ttgtgaattt
     4321 ttttactggt tcgttgttat attcccctat cccaagtttg ttattctgat tcattcctgt
     4381 tacatatata ttgttatttt tatctactac tactacagta tttcctttta caattatatc
     4441 ttttatgtct tttaatcctg tatcatagtt acttgtgcca tctatatttg ttgaattccc
     4501 aaattgccca ctattagcaa tcccttttcc atataacttg gaattactat gatatattat
     4561 aaaattactt cctaaattag aactctcaga attaatacta gtataacttc tatgtccagt
     4621 aatggttgag ccaaatgttt ttggatatcc agtaatatat gaacttcctt tcttaaaaaa
     4681 taatatatta tttgacatcc ctattacttt ttcaattcca ctaaaatcag tctcttgtat
     4741 aaaataagta ttatgatttg aatttgtagc ctcaataaaa gttccatatc ttgaataatc
     4801 agatatactt ggactgctat ttaatgttct taaatatcca tgatatcctg ttaaataaac
     4861 ataacctcct tcgtcaatta ttgcacaacc atcataaaaa ggaaaaacat tagttatttt
     4921 aggtaacgtt ctaagtccat ttgatgtaga agatgttgaa tttgttctac attgagatac
     4981 taatgctttg ctttggttac tattatttcc tttacctaat tgataattat aattgtatcc
     5041 agaaacatat agtttccctt cactgtttat catatataca gaataatcat atgctgctat
     5101 cttttcaata ttacttattc cctgcttata tggataatta gaagtttttt ctatattatt
     5161 ataaggatta tttccccatc catatacttc ttttgagtga tttaaagcta tgacataatt
     5221 gcttcctaca caaaaatcct ttacattctc tatttctatt tttgtaaata tatttctatt
     5281 aacttcatct cttaaaccca attgtccatt actatttaaa ccacatgaat ataatgttcc
     5341 attattctta agcacaaaag ttgagtttcc gtttcctttt atatccaaaa cattatctat
     5401 atccacctta gtcataactg acctgttaac agtatctccc aatcctaact gtccataatt
     5461 atttaaacca caagcataca atgtaccatc attcataaca ataaacatat tgctatctgt
     5521 taactctatc ttcttataat cttgaaaatt atcacttaac tttacattta aaaattcact
     5581 attgtccttt gtaacatata aataatcatc attaaccaat aaacctgaat aattatctaa
     5641 atttgaagat tgcacatttg aaaactcata cagaaaagtt gtatgaaaaa atgaaccaag
     5701 ttgacctgct aaattacagc cagatacaaa tatctctttt gaaccattta ttaaaaaatt
     5761 aaattgagaa ccacatttaa tatcttttat attttctaca ttaaccttag caaaagtatt
     5821 tcttgatgct acatctttat tggcattggc tagttgacca taattgtttt gtcctgtact
     5881 aaacagtgta ttatcgtatt ttaatatcat agtatgatta tttccacaag aagcatactt
     5941 aacattattt atactagaaa gagtaaatac aattgaatta ttattatttc ctgtaccaag
     6001 ctgaccaact ccattatagc ctgtagtata catagttcca tcattcttaa taagaattgt
     6061 atgactacta ccacacgcta tctgttttac attatccata ccttctattt tattaaatac
     6121 acttttattt aaagtactat taattcccaa ctgcccattt gaatttaatc cacatgcatg
     6181 tgctgtccca tccattttta tgataaatac agattgatta taagtcacta catctttcac
     6241 actatctata tttactttag taaaagtagc tctattgtta gtatcaccta gtccaagttg
     6301 accacttgag tttaaacctg ctgaatataa tgttccatca tttttaagca caaatgtaaa
     6361 gtcataacca catgcaacag tttttacatc atctattggt agcttagtaa attcataata
     6421 tactgtagac tcaattacac ctaattgacc tacattactt gttcctgctc catatgcaaa
     6481 tccatcttta gttactgcta cactgtgttt tgaaccacaa gatacatatt ttacattatc
     6541 aatttttact ttactaaaca tccttctctc tgacgaatct tttccaagtc ctagttgaaa
     6601 acctgtatta tcgccacaag catataaatt accatctgat tttaaaccaa aggtactatt
     6661 acttatacca gtaaaaaatt ctacaaaatc ataatcatta tatcttttat caaaatcatc
     6721 aatcaatatg ttcttatcat taaaataagc accacgctgt aaaagtttat tttgcttcat
     6781 tattagtact acctccttta tttatcttat ttcatatgaa tatttactta gagttatatc
     6841 tttttgttct tttaaataag tgttactaga ttttgtctca acaaagttat tatccatatc
     6901 atgtatatat ctaactctat ttgcactaaa tggtaatact acatctctaa aaatcacatt
     6961 accaatttca cctgttatac attttctatt taaatctaac ttaagccctt ctatagctac
     7021 tatgttattt ccatttgcta atgtatcata ccaataacca gcctcatcta cagttacttc
     7081 tgactctaat tctaggtata ttaatatatc tagcactttt tcagataatt tgttatagct
     7141 attttttaat actgatatat cagtttgtat ttgaccactt ataacatcat caaaagatat
     7201 attttctatt tttttaacta cctcatttat agctcctgat aaatctttag aatctgtagt
     7261 taatatatct gtatctccaa tttttacttt tatttctttt atatttttat caaacttatc
     7321 atctaaatct tgaatatcct tttgagttac aaaaaccaac gtcgggtcta cttctaatgt
     7381 aacatttgaa gtattggaca attgtaaaat catttttaca accaaatctt taatactgcc
     7441 atcttcagca cgtggcttat acgtctctgg atacttacct atagctatta tattattctc
     7501 attatccaga acagcagctt ctcttatcat aaatccacca actgttcctg gtaaaatagt
     7561 ctctataact atccaattag gatttttttc atcaattctt agagaattta tatttccttc
     7621 ccaaactaca ttctttaaag cagtttgttc ctctgttggc tcataatatt ctccattact
     7681 atctccaact tgaaacttgg ttaatccaac aagctctcct agtgcagttg catttgctat
     7741 tttttctcta ccaattttgg ttaatattat ataaaatttt tcttccaatt ataccacctc
     7801 cgatttagga taaactacta cattttctac gcctgtatca tttgcaatag ctatattaat
     7861 cttacaatta ttttcaatgc atttaatgct taaaggataa acctttattt cttctctact
     7921 taacattcca gctccacagt ttacaacagc actacatttt ataattggtt caaagtatgg
     7981 atgaactttt acctcttcac tacataccat acctccacca caatagacca tacttttatc
     8041 tagtattatt ggttctaaat agtgtaataa gtgagcaggt tttactcttt caatcatctc
     8101 gctacaatct aaaagagttt tatagtcaca gttatttgct ataaaactca atacgaatgt
     8161 aaattcatca ctataaactt ttatatctgt ttctgatttt gtatatgcct cacatatact
     8221 ttttataact tcaatagtac tagtacctct gcttctcatt ttagctttta tattgctacg
     8281 tcttgtgtca aaatcaagtt cttttttttc aatgcagagt atgtcttccc acatatctaa
     8341 tccccatgtc gctgtatcaa caaataattg gttaaaggta tcatcaatat cttctttaag
     8401 aatatttaat tctgtatcgt atgcaccttg tatctcctct acaatgtaat ttctatcaaa
     8461 tgatggtagt ttatcaatta gcttcattga ttttccacct cactaaaatt aacagttgtt
     8521 acactaggta ttttatcttc attaatagtt atattatctg tacttccatt tataagtaga
     8581 ttacttaaat cgtgtacacc agtagtattt ataagtattc ccatgacttt tgtatagatt
     8641 atctctcctc taatatctct aaagtatgta tttatacttt ctaggaaaga ttcttttaca
     8701 ttgtctaatg tatatccatc ttctagtttc attactgcac ttatacttat ttctattggt
     8761 aatggtgtca caactgttat agttggtcca ataggcttct cttcatctat atgttgctga
     8821 cacctttcaa tcgtttctgt atcaacagct tgattatttt ccccaaatat caagacctta
     8881 actgttcctg gaccatccca tcttggataa accttaacat tatagactcc atctacttcc
     8941 aaagcccatt cttcatagtg agctttattt ccacttgtag cttgatttct ttggatttta
     9001 taaaatcttt cttttagttc ttcatctgtc tctatatctg taccaccttc aaaacttctt
     9061 gtgttagtaa tctttgttac tccagatata ttatcaacta gtttaaattc acaatttgta
     9121 ggtaaattat atttcttacc aacttccaga gcttgaactg ggctattgtc accttcttca
     9181 ctaccaatag ttacatcttt tattactaca aatagtaaat ctctatatga tattattgtg
     9241 ccattattta ttacagttcc tttctctcca ataaattcaa cttctccatt tgactctgta
     9301 cctaactttc tatatacacc aaattcattg actcttttat caagaaactg gttatatgtg
     9361 tcttgtataa aagccatttt atgtatcttt gaaagttcta gatatatctt cgaaagttct
     9421 agattatttc cagataccat gttgtttaga aaagaacctt ctcctttata aatatcaaga
     9481 ttaatatttt caagagttct attttttatt acttcatatg tctggtcact atacatttat
     9541 ttccacctcc ccatagatag tggatacttt tacatttgca ctcaatacgt ctctgttaaa
     9601 gttagcactt tttacgttta cttctaatat atatggattt attagaaggg cctcttttat
     9661 gaatctacta gcttcacttt ctgtaagacc ttttgtatat ttttgtccta ttagttctga
     9721 aagctctgtc ccatattcta aagagtatat ctcatgttca tatctatttg tctttataca
     9781 cttgtatatc caaactttta aggcttcttt tttttcaatt attttaaagt cacctttttc
     9841 taaaataggt tcatcttttt caaaatccca tgccacttca cgaaagattg gcaattcttc
     9901 tgttttaggt aagatataat cctctgggac acctataaaa ggaaatattg tactcattat
     9961 aagctcacca acttacttac aacagcaaat ttttcgccta ttctaaacat tattaccata
    10021 tccccagatt caaagttatc tataaaagga ttttttactt catgctggtg ttcttgattt
    10081 gtttctgtgt caaatgtttc tagctgtcta tcaatcatcc aactatctat caaaatatct
    10141 tctttttcta atatgatgtt atttgtctct atttttaaat ctggtaattt acttttaatt
    10201 tttccaataa aaaaagaagg ttgattatga tactttcctt cttctcttat tattcctata
    10261 aattcattta ttggattagc cattatatca ccatcctttt ataatactct cctagctgta
    10321 ttaaaatctc ttctactact taacttactt attttaacta catcaccttt ttgtggagca
    10381 tgtaaaaatt ctccattacc tatatataat ccaacatggc tgactggatt atgaaaaaat
    10441 actaaatctc ctgcttgaag atttttttgt tctactttct tgcctttttt agattgttga
    10501 tttgacgttc ttggcaaact aacattaact tttttaaaac agtagaccat taaaccagaa
    10561 cagtcaaaac tacttggtcc atttccaccc catttataag gtttacctag atgttttttt
    10621 gcctctgata ttactatttt agctttatca gatacattac cagaataacc accaataata
    10681 atttttcctt ttcttcttcc aaatttacta gcttcttcaa catttccaaa taaaatatct
    10741 atatggtatg ttccattttt ttctatagtt attgcaggtc ctacgtcatt tactttatag
    10801 actccatcac gaccagaaac tcctgtaatt acctgtattt catctccata gttcaataaa
    10861 ggatgtttat ttaaaaactc ttttgtataa taagtttgct catatttacc aaccatagga
    10921 gcagcgcaag tttttttaga tgggtcaagt tttttctttc tacaatctgt atctccacct
    10981 tcttcttttc taggacagta agctgtaaat tctgctgtaa actcttttcc tcctgcataa
    11041 tcttctcccc ctaaattact ttcttcctta ggttcatcct gtcctgctga cttttcatcc
    11101 ataagatttt gaaaattaag ttcaagctca atttgatatt ctccattttg ccaagtatgt
    11161 ttgtctgtat ctatataaaa tagtcctaca agctttgtat aagaatcttt aacttttact
    11221 cctctaccag ttatacaact tacatctcca taacctttaa gagaacagct tttttctatc
    11281 ccattaaact cgctatcaat atctacatct tgattttctt gttgctgaat tactttttgc
    11341 attattacat ttacttcctt aaaaatttca ttatctattt tttcgctaat cttgcttcca
    11401 tactggtcta ccactattac tttatttttt acattttcca tgctctccga aaaggtggta
    11461 tttataatat taaatccctc ttcaaacata acacttaatg taacagttcc tttttcaata
    11521 acattaaact tatctaaatt agcctctatc atatactttt tctttgtctt tttacttgcc
    11581 tctgtatatg cactcattat tgtgtcataa ccatttactc ctataaacat ctttgtatac
    11641 ttgacattgg tctttgctat tgttccaact gaaagcctat tttcagcaaa tacttgcttt
    11701 gctatgtctt caactaactt atctttaaaa ttgtatgaca cttcactttg tgtaagtaaa
    11761 aatcccatat ctttagatac aaaactaatt tcattactgc ttgaatcttt agacctatta
    11821 attatcattc ctctaaagag ttctttctca tctacataga aacagactgt actagctatt
    11881 ggtatatcga tttgttggaa atttacatca aatgatgatt gtattattga aaactctagt
    11941 gtccttgatg gagatttata atcacctgac catgatacct tgtctactat gtcagttatg
    12001 tcatatatac ttccgttttt tatgtgtacc caaatcttaa taataaaaaa tcacctgcct
    12061 tttcatttaa ttctatcagc agttacttac aacttattat ctataatagt tattgttttg
    12121 gagttacttt tggtatcttt attcttttat actcttttaa atctaatgta aaatatacat
    12181 ctcgagtccc atctctttct tcataactaa acccttcaat tatgacttcc atatttatat
    12241 ttgtttccgt aattgtaaat cttaatataa aaccttcctt catccatttt tctatcttat
    12301 ttacacaatc atatggtgat ggaaaacctg tatagtcaca gaaagtgtag tctttatttg
    12361 gaaaaaaact tgatattgaa atgcttttaa gagctacacc accaaaagtt gctacttcac
    12421 ctattttgag tatactagaa gagtttacag cagcttttcc attaatacta aaggaagatg
    12481 gaaatactgg aaatctaaat ctatcttctg cttgtctaag ccacatttcc attataagac
    12541 accccctaac tgtgccaatt ggatacttgc aactaatttt gatgttatct tttctatatc
    12601 agcttcttct ctaatcacta ttgtatcagc taacttagct atgtttatat ttccaccatt
    12661 ttttctgctt ctatattgat ttgcttcttg tttagttaaa accatttcac cttcatggag
    12721 tctggtttga tagtcattat aaggaacata gttcaatcca aatgcattat tacctttctt
    12781 acttgaataa gctttaccag aatcaccagc ctttgttcca gcatatggat tatctttgta
    12841 atttttttta ctcttttcca ctagatttaa aactgttcct gtgatgcttc cattttttat
    12901 cttattaatc atagtttgcc accatttgca tatatcttta actattttac atactttatc
    12961 taatatgtta aaaaaagctc ccaatattgg ctctataatt ttccatccag tttccaacag
    13021 aggtcctaag gtctcccata cagttttcca aataacaccc agtgtctcta taaaattgtt
    13081 tatttgaggt gcatattcac ctaaaaaagt aaaaattctt gtgataatag gactaacagc
    13141 ttcaactaca ggtccgatta gttggaatgc tgtagttact attgtaccaa atatacttat
    13201 taatccttgt gcaattggtg atttattgtt tatactatct agtaaattat ttactaagtt
    13261 tactactgga agtagtgaat ctattaatgc tgtaaaatca atattttgta atagtgtact
    13321 tcctgcgtta acaattgctt ttggtgcatt ccctatcatt gttgctgcat gattttcaaa
    13381 acgttcttgc ttattttcaa ccatcatttt acgcttttca gcctcattag aaattcgata
    13441 ttgagtagaa tcagaaaact cttttccatt ttttacactt ctagattgca tctggtcata
    13501 tttgttgcca ataattatct taccattcat tacattgcct attttaccag ccattttttc
    13561 agataagcca gctaaattcc ctaatccact ttctataaaa gtttttattt tctttagtcc
    13621 ttgacctaaa atatcatcaa aaggtttaac aatactcttc aaaccattgt taatgcttgt
    13681 ttgagctcct gatattatac ctggtattgt attactcatt tctttagcta accccatctg
    13741 ctttacactt atgccttcca atgcttcatc aaaagtttta taattttctc ctaccagagt
    13801 attaagagat tccatatttc cttttgctgc acttaagaaa gctgctgata catcttgttc
    13861 actacttgta ttcatattta aagctctaac atctaccata gcttgagtta tatctttttc
    13921 ttttttgcca ccagtaatat tactaatcat tttagcttta gttcccattg caacaacatc
    13981 ttcagggtca aaaggcgtat catttgccat atttactaaa ctgtcataat acttatcaac
    14041 atctttttgg ctagtttttc cagtatttct tttcattaca ctttctagaa caactttctg
    14101 attttgttcc tgaccaagta agtcaacacc ctttttcaca gcgacaccta tagtagcacc
    14161 atttgtcaaa tcagatatct tcccttttat cgaaccaagt attccacttg ccatatcctt
    14221 tacagctatt gcaccactcc ataccttacc tgcaaacaat ctcaatttgg gaaatacttt
    14281 acttataatg ctacttgcca tatcttttat agctattaca ccactccaaa aagtattttt
    14341 aaacaacatt aacttaggca aaattctagc catagcacta cttaccatat ctttaagatt
    14401 aatacttcct tgataaggac gtttctctac tcgttttaat tttcccaaga tactacttat
    14461 cactctctta gtcatatctt ttgcttttat tacgatattc caaatcttgc caccaatctt
    14521 ttttaaattt ccaagtaatt tatttttagt tttttctact tcatcacttt tagctttaaa
    14581 tattaatata atcataatat ttgaattaaa ctcatttttc ttttgaatca acttattaaa
    14641 ataatcaata atcatagcta tagttattgc tagtctcata atacttccat ttaaacttcc
    14701 aataactcct gaaacagcat ttatgctaga acttaatttt gccatagttg aacttacact
    14761 actagcagta gtttgaactt ttcttaacga ggaatttaaa ctgtttactc tactacttgc
    14821 acttccaata gaatttttta tgctactagc cattacatta gtggaaagtg aaacagccac
    14881 agttgctctc ctaaaattat cagttactga tataatttgg ttcatttttg gtgtgtaatt
    14941 atctgtaaga tacatattta taccttcttc tctcacattt cccattacat accacctcct
    15001 gtcaaaggac acactccata tccatgtttc attttttcaa cttcttcctc aatttctaag
    15061 gcgaaaaaag cttcaataag ctttaattcg cctttatcca ttgcataaaa aagggacggt
    15121 cttatacctt ttttcttcca ataatagtac attattgtag tcttggtatc cgtccctatt
    15181 agttttttac ctcttctatc actgcatttt taccataacc cataacatct tgaagtattc
    15241 tatataggtt ggtaatttca cctggtaata atagttttct tgccaattct tttggtgttg
    15301 gagccttaaa tttatccatt agttccttat ttttaaataa aagctttcca tccaaatcac
    15361 aaacaccttc tataattgta agcatctgca tctccggtaa gtctatatcc gttttcatat
    15421 tttcatcaat tttaagacaa gtattttgta tctcttcatc tttacttatt gttaaagctc
    15481 tacacattac tttaaattcc tgcccaaata tagttgataa tctcttaagt tcaactatag
    15541 tacttggtct ttctaagttt tctgtatctg catttaaaag taaatctact atgtttatat
    15601 tttttgataa tccattttca ttcattatta tttgctcctt tatcaatcaa attcaatcaa
    15661 ctaacttttt tacttattaa ctacctaaat ttattaagta tatatctact aaatatcagt
    15721 ataaaaccaa atataaaact aaattatatc aagaaaatca tactcagtaa atgtgaaagg
    15781 tgcttctatc tctcctttta ctccaacctc ccaatcagct aaagttaaat catcaaatgc
    15841 tatatttttt actgctattc tttctgctcc ataagaatct gggtcattta atttagatat
    15901 agcaacaaat ctaggttcag aacctctctt tatcttttct ccaattaact tgtgcattct
    15961 tgaactaaca tggtatagag ttattgaacc ttttccttta tatcccatat actttgtatc
    16021 agtacccatt tgacctgcta ttataatatc ctcttttgta aattccatct ttgcttgaaa
    16081 cttctttact tctgctactt tgtttccatc aagccaaagt tctccccaag taccactcat
    16141 tacatttcta gcttccatat ttgccatatt aataatcctc ctatatttct attgataaat
    16201 ctatatcttc catagcatca agtactttta tttttgcttt taaaaatact ttagaacctg
    16261 tgttagcttc ttttatttct tgtaatgtca tataagataa atctactcct tttgatttta
    16321 aatacgattt ttgtgcttca aaatctattt caacagtaga atcagattct ataagtgctg
    16381 acttttctaa ttcttctaaa taacttttta tagctactat caataaacat ttgttgtcat
    16441 aactgttagt aacctttcct atatagtcat ctattatcac ctttcttatg tcactatgta
    16501 taatatctaa agtgtcaact atttttattt tctggaacat ttctcctttt tcttctgtta
    16561 actcagttaa agaatttact cctcttgcaa ttcttatagc ccctgcttcc ttaataagta
    16621 taagctctcc tttattaacc cttgattctg catcaacttt cgtcatctta ggtatatcaa
    16681 ctacatcact aagcttagta taagttactg attgacttaa aggtgttcct gctataagtc
    16741 cagccaccct acttgtaaac tcatcaacac tgtatttctt ttctccaact aacacatctt
    16801 ctgtagtaaa attaattatc ccttcatgat ttcctacaac ttttcctaat acagctttaa
    16861 ccttaacctt atctatatct ctaagtttaa ttatccaatt ttttatagca gtcttatcag
    16921 cttctactgc ttttggcata catagataat taaattcctt agtctctaaa aaatctaatg
    16981 cagtttgaat atctgcttct ccttctatta cataaactaa taatttattt ggagtgttaa
    17041 catttcccat caaagctaaa tttatatact ctttattttc agcagataaa tcaactggta
    17101 tatcctcttt ttcatgtatt tcattaagac ctagtgcctt agcatcttta agcaccattg
    17161 caattattcc tctagctgaa cgttctttaa cagttgtagc tagctcctta aatgatatgt
    17221 tgatacttgg taatcctata gccatactat cactctccta aatttaaatt gatttcttcc
    17281 atattttcat atactacatc ttcttcatat acactatcca gataatttag tgtaattgta
    17341 aagttcaata cataaattga ctcttctttt ttaatacttt gttctagctt tgttatattt
    17401 aattttctgt ctgttacctt tatatttctg ttgaataact tatttaattc atctgaaatt
    17461 tcatacattc taatactctt tgactgtggt tttggtaagt attttataga gataacaata
    17521 tctttattat tcgtatttaa cattataggt gtacaaataa ctgatagtat acttacaaaa
    17581 aaacaagacc cttcagagcc ttgcacactt tcatcatcaa taaaaatact ttcagtaaag
    17641 ttttttttga gaatctcaat aattgtttct aatatttcct tgtatttaag caaataatca
    17701 ccacactatc actaaatatt cttgcttaat ctgataatct tactataaat gatataagtc
    17761 atttttaaat atacatctca tttattcctg tcaaatatct tctatatttt taggtaatat
    17821 tattcttttc aatttatcac ctccttcaat atggatttct ttcacaataa cattatctca
    17881 catattttat tgcctagtgt tgcaatagtg tatataaagt gttgtaaagt tgtcatattt
    17941 tttataatat ctcactcaat ttaaatatca tttttttctt taaattgtag caagatgttc
    18001 tatctaagtg tagtttcata gaaatatatg tcatattgtt ttttgtctta ctattataaa
    18061 aaagattaaa aaattccatt tctatatcat ttagacatgt tagagcattt tctatcttct
    18121 ctttttctat ttctatatct tctttttcca tttttaattt tcttaatctt tcctctcttt
    18181 ttaacacctc attttccaca gaagaactta tgttatatgt gttactagtc ttttctgtat
    18241 ataccattgc tccacatcca gagtattcat tctcacatct ttttatgtcc aaattaatat
    18301 tctttatctt tatatccaaa tacttataat tatgtaatct ttcatctgtt tctttaaata
    18361 atatatcttt tttgttttcc atagtacttc tcctttttta tatacttatt attagcttca
    18421 aatttacaat aatctcttta gtgcacagtt ttttactaat ctcacaactt tcaagaacat
    18481 attgttctat aactgtataa aaaaaacttc ttctatattt tgttctaaaa tactagaaat
    18541 cttcttagcc ataagaagac caggattttt tatacctctt tctatttgag atatatagtc
    18601 atttgtaatt ccagcctttc tggcaagttg aatttgtgtc atacctttgc attttctata
    18661 atatttcaag ttatttttaa acattgtttt atacctccat taccattaag aatattttgt
    18721 tcttccacac ttagattata tagaatcatt tgttctttat caatagattt tagaaaaaaa
    18781 tattctttta tattttatta gttttatttt ttagaacaat ttgttataat ctatacataa
    18841 ggtggtgtta taatgtttgc caaaagatta agagaactta gaaaggaatt tggattgact
    18901 caaagagaac ttggagaaaa agtaggcgtt tcccaaagag tcttagggta ctatgaaaca
    18961 gaaaacagat ttcctgatga acacatttta aacaaattgg ctgatgtatt taatgtatca
    19021 gttgattacc ttcttggaag aaccttagtt aaggaaaata ttgatacagt agctgcacat
    19081 agaaaaaatc cacatgaaga gttacctgaa gaagcacaag aacaacttaa tgattatata
    19141 gaatttttac taaataaata taaaaaaaaa taaaccatat gatgagcagt ctatttttct
    19201 gctcttttaa tataatttaa aggcaaacat acattctatt ttagggggat ttttatgaat
    19261 aatttggata agctttttga attagcttct caagaagaga taataattca ttataccact
    19321 tatattgcag gtgatttaga agggctatat ataaacaaac atggtattaa aattatatca
    19381 ttactcagta atttaaaaca aaactcaaaa aaattgacat ccatcttagc tgaagaactt
    19441 ggacatcatt tcactagcct tggatactat gtatcctctt ataacgatta ttatacaaaa
    19501 attattatag ataagtgcga aaataaagcc ttaaaatggg cttgtgagtt cttaattact
    19561 gaagaagata taataaacat aattaattct ggcatcactt gcgtttatga aatggcagac
    19621 atacttaatg tggatattac tttctttcaa aaaagattag aatttttatc attaaaaaaa
    19681 cagtctttac aacttggaaa taataaatat ttaatattaa ctaatttgcc gtatttctac
    19741 atatttgatc caatttcata ataaaaatag tagtaatttt aactggctta acagtaaaat
    19801 ctatatattt attttaacaa attttggttt ctataaattc aatattaata tataataatg
    19861 atatcatatt atttaaaatt ctaaaaacaa atatatgctt ttgttaagaa aggttgtttg
    19921 atgaatgtat aaagaaaagt tgtttgataa ttattttaag ttcttagcac tattattttg
    19981 gccaattatg tggtataagt ggatagtaat ttcaaatggt actcttgaaa atatgttatt
    20041 taccatatat gcaatcgtcg caattatttt catcatttta tattcggtgt ttatgataaa
    20101 atataaagac attacacaaa tagatttctt ttacaggata agtactctac ttgcttttat
    20161 atttacactt tttagctttt taatataccc aaagagttta tttttcttgt atttaaagat
    20221 tatttttact ggaatatacc tttattattc aatagtaaaa actcttaaat ttaaagatga
    20281 tgaaggtgta gtgggtatta tgagctcact tttattaatt gtaataactt tgttctacta
    20341 aatttaattc tatttgactt aatatggctt taaatattag acatctacat tattttctga
    20401 tattatttaa gatggtagtc ttatattaat aatgaaatac actatgaagg agttaactat
    20461 tatggaaaaa aaagttttag caacagttgg agaaaaagag ataactaata ttgatattga
    20521 aaatgcatta aaatcattag acccttatca agctatgcaa tttaaaactg aagaaggaaa
    20581 aaaacactta ttaaatgatt tagtgaatca agaattattc ttcttagatg ctaaagaaga
    20641 aaaattagat gaagaagaaa tatttaaatt agaaatgaaa aagatagaag aaaatgtatt
    20701 aaagcaattt gctataaata aagttttatc tagtgttaat gtaacagaag atgaaaaagt
    20761 aaaattcttt gaagctaata agagctcttt ctctaaacca gaaagtgcaa ctgctaaaca
    20821 tatattagta gatagtgatg aaaaagctaa agaaatactt gctcaaataa aatcagaaga
    20881 aatatctttt gaagatgcag ctcttaaaca ttcttcttgc ccatctaaag atatgggtgg
    20941 tgatttagga acattcggta gaggacaaat ggttcctgaa tttgaagaag ctgtgttctc
    21001 tatggctaaa ggtgaagtaa gtgaaccagt taaaactcaa tttggatacc atataataaa
    21061 attagaagac cttcaagaaa gtacagagtc tacatttgat gaagttaagg ctgaagttga
    21121 aaaaagttta ttatatcaaa aacaaaatga agtttatgga aataagataa atgctcttaa
    21181 tgctaagtac ggaaaccttg taaaatacaa cgattaatca taaggtcata tataaaaaag
    21241 ctgtcttttc acaatattta ttagaaaaga cagcttttta tattaaagtt taaatattca
    21301 gtttcatatt atcattcttt tattgtaaag aactcagctt aaaagtttaa ataagatttt
    21361 taattatcat tagctaaatt tttatatcca cgtattatct cataacagta acctattaca
    21421 tctcttcttt ttattatccc aataaatgtg tcttggtcat ctataacagg aatgaaattt
    21481 tgatttaaag attttataac taaatcttca atatctgcgt tgatggaaac aggagaatta
    21541 tccatttttc tatttatatc tgtcactgga acatcttcca atccctttaa gtccagattt
    21601 aaatcgtttt ttaatgtcca taaaaaatca ccttctgtta tagttccaac atatttacca
    21661 tccttactta ttattggaat tgcagagtat ttgtgatatt ccattttttc tagtgcttgc
    21721 cttatagtgt agtcttcata tatatatgct acttcacttt ttggtgttag aaagaataat
    21781 atgttcatta cactacccac cgttactgaa aacttataaa tattactcaa tatttataat
    21841 gttacttttt tgcagtaact ttctcctttc ttattttcta agtattctta atactatacg
    21901 ctgtaacaag cgtttttcct ttgctcattt taatcttatt atgctttatt aatattttta
    21961 tatggtctat cagtatcact tattctcttt ttagaaaatt tactaaaaat taattgactt
    22021 aaacttagcc ataataattt tactctacac tattaatttt aacatattta tctcattatt
    22081 aatgatttta ttttaataat tttaatttat tttgctatta attttattta caaaaactga
    22141 aaaaaccgct aaatgattag cggcttatct ctcatattac tagaacacta atatatatga
    22201 atttttaatt ataatctaac tacgttagca gcttgaggac ctttagctcc atctactact
    22261 tcgaactcaa cgctttgtcc ttcttctaat gttttgtatc catctccagt tatagctgag
    22321 aaatgtacga aaacatctcc ttcaccttct acagatatga atccaaatcc tttttcgttg
    22381 ttaaaccatt taactacacc agttttcatt aataaatcct cctaaaaaaa cacaaatatt
    22441 tagctactct tttctgtaac cttattacta ctctatcata tatagctatg tttgtcaatt
    22501 attattattt ttatacatta ataatcaatt tttttacttt ataattttaa atgattatta
    22561 tcttttacac tttatttttc tttataattt tttaataatt tatttttatt atatggaatc
    22621 ttatggactt atcactccag cctaaatccc atggcaccaa cattctatca gtattatggc
    22681 aagttaccaa aggatatcct cttgagtcta atcctgtcac tgtcgaaaca tgtgttattc
    22741 tgcctttctt ctcataacca acaaaatccc ccggacgtaa attataagct tctttgtata
    22801 cttcttcata ggaaccttta gcaattaatg aacctcttcc actatacagt gcataatttt
    22861 taaaagcttg tgcatttacc catgcttttg tacctgctct ctcgctataa ttccaaattg
    22921 agttcttttt gaaccttcca ctttcaaaca ttatctgtga tgcaaagttt gcacaatctc
    22981 caccttctgg attataatcc ctatagtttg gattaaattt cataccatgt tcttcatcag
    23041 ctgcggctcc acagtattta tgtgcataat ctatagcctt tttttgttca tctgtaagct
    23101 gtaactctac accatcttgt tgcccaatat agtttcttat atcctcagat tttatatttt
    23161 ctaaatttaa agagtctgca aaagggtctg tgtaccactc tttagttatg acatattgat
    23221 tatctataat ttttacattt agataatgat atgtacctat tctaaaggca ttttctttgt
    23281 ccttttgatt ttcatatgaa tacttatatt cagtcgatac tgaacagatt ataccataga
    23341 ggtctttttc tttttctttt atttttttaa tcttaaccct tgtctttatg tctttaaatt
    23401 ttacaccttg cttactagac cagttttcta aatacttcat cttttttact tcattttcat
    23461 aagcccacag accaaatttt ttatccgtct cataaagacc ttttaaaatg ttttcatttt
    23521 tctctaatat tgccttattt ctgtagtcaa ataagttttc aagcaatagt ttatattggt
    23581 cttttacatc atcaccttca acatttgcag attccatgac tttttcttcc atatttttca
    23641 tacttaaatc attgattaaa aatacacctg ctgtagtagc taaagacaaa aatacaaatg
    23701 ccattatacc tttgatgtac ttattcttat ttttcagtat tttcaaagct acacctccca
    23761 aatagtacac cttataaatg aaattacaaa gaagattctg tcatatacaa taattttcat
    23821 atttaataat tctctatttt attatgtcca atattaaaaa ttaattgtat caatacactg
    23881 ttttttttac aattttttat tttataatct acttttttct tttttttata gtaatatata
    23941 tgtatataaa tattttggta tcattataat taatacatac taatggaggg ttttttatgc
    24001 aaaaaaaagt cgcagcaatt aatgacttat ctggaatagg aaaatgctca ttatcggttg
    24061 ctatacctat actttcagca ttaaaggttc aatgttgtcc atttccaacg gccatacttt
    24121 caagtcaaac tggttatcct gaatttactt tcttagactt tactgatgaa atggtaaaat
    24181 attcaaatgt ttggaaaaat ttaaaagtca attttgatag tatatatagt ggttttttgg
    24241 gttcaaagca tcaaattgag atagtggcta attttataaa tgattatccc aatgctttta
    24301 tcgtagttga ccctgtgatg ggtgataatg gagttatgta tccaatattc acagaagaaa
    24361 tgagacagga aattaaagaa ttagttaaac attcagattt aactactcct aatttaacag
    24421 aagcttgttt cttaactgga aatgattata ctaaaagcga ttataataga gatgagctta
    24481 tatatattgc aaaatctgta tctgatttgg gacctagcaa agtcgttatt actggtatat
    24541 tagaagatga taatatatta aatttggctt atgatagaga taatgaccat gtattcttta
    24601 catcagtcaa atacaataat tgttcttaca gtggaactgg agatatattc acatctatac
    24661 tttgtggtat gttagtaaat aaacatgatt tgggagtagc ggttaatact gcaacagatt
    24721 ttatatataa aactataaat tacacatctc aatttgatac tgatagaaat gatggagtta
    24781 tgtttgaaaa ctttttatca gatttaacta atatataaaa tcaaaaaaac tatccatttt
    24841 ataaatacct ataaatcata aaatttaggt atttacaaaa tggatattat ttactaaact
    24901 attggtattt caattgttac catagcacca ccagttttca cagaattttc aatatataaa
    24961 attccattat gtttttgtac aatattattt acaatataaa gtcccattcc gtaatgagat
    25021 ttagaatttc tactcaaatc tcctgtataa aactgttctg tagcttttga caaatctgct
    25081 ttagaaaaac cacttccaca atctgtaact acaaatttta agtatttatt acttactgat
    25141 acacttatgt atagtttact ctggcttggt gaataatcta tagcatttga aattatattc
    25201 ataatagctc tgaataacag ttctctatct atagttatat ttttaggcaa attatcttgt
    25261 ttataaaact ccacatctaa gtttttagta ctagctaatg cttctatttg cagtaagaca
    25321 tcttttataa actcactcat attaattgct tctttattta atatatagcc attctctgtt
    25381 tttgatatat ctattaaagt attaatatat ttttctattt gagtgacacc ctttgagata
    25441 tattccatgt attctaataa ctcaggattt tcatttattt caattaataa gtcagtattt
    25501 ccatgtataa ttgttaatgg tgtttttata tcatgtgcca gtgcagatat ctgttccttt
    25561 ctattttctt ctagcatcca ttgtttttct aaagaattct ttaacgccaa cttcatttta
    25621 tccatagaaa aaaggatatt gttaatttca taaattttac tagattctac aacaaaatct
    25681 aaatcctgtt gttctatttt ttctgtagta ttttttaaaa gctccatttc aatattaaat
    25741 ttcttgccaa atacttttga taatataaca atttcaataa aaaatattat actaaaaata
    25801 atcataccta acatctctgg ttttggtaaa tatgctctta aaactggaga actatactct
    25861 gatactagtt tgtattttat tataaaaact tcatctttct taaaaaactt tatataactt
    25921 tctgaatatg catgcctttc ttcaatatct ctcattaaat tccatacttg ttttgattct
    25981 tttttattaa aagtgcctga gatcaaattc ccatctaaag tataaacacc atactcgtat
    26041 aaatttggaa ttatatcttc aattgctttt tcacttgaaa taatcttttc tttatattta
    26101 tcaaattgtt tttctgaata attagctggt aatattacac ctgatgataa taatacagaa
    26161 aatgataaca ttaaaagaag tgcaaatgaa attgtcataa caaagaataa agataaatat
    26221 tttataaaaa tagttcttaa cttttcaact tttttgttgt tagatttttt acttctaagc
    26281 atttgtgcca tatcctccaa atattttaat taattcataa ttaataccta accagttttt
    26341 atttgattat t
//
LOCUS       DACTGW010000032        26272 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.032, whole genome shotgun sequence.
ACCESSION   DACTGW010000032 DACTGW010000000
VERSION     DACTGW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.032"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            40..246
                     /locus_tag="I9573_001054"
     CDS             40..246
                     /locus_tag="I9573_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795682.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFNKID"
     gene            complement(557..1300)
                     /locus_tag="I9573_001055"
     CDS             complement(557..1300)
                     /locus_tag="I9573_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAT4795683.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            1466..4033
                     /locus_tag="I9573_001056"
     CDS             1466..4033
                     /locus_tag="I9573_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAT4795684.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNNSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAVDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            4263..4667
                     /locus_tag="I9573_001057"
     CDS             4263..4667
                     /locus_tag="I9573_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT4795685.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4691..5188
                     /locus_tag="I9573_001058"
     CDS             4691..5188
                     /locus_tag="I9573_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4795686.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            5191..5592
                     /locus_tag="I9573_001059"
     CDS             5191..5592
                     /locus_tag="I9573_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HAT4795687.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5610..6098
                     /locus_tag="I9573_001060"
     CDS             5610..6098
                     /locus_tag="I9573_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4795688.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            6125..6880
                     /locus_tag="I9573_001061"
     CDS             6125..6880
                     /locus_tag="I9573_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT4795689.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6880..7743
                     /locus_tag="I9573_001062"
     CDS             6880..7743
                     /locus_tag="I9573_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAT4795690.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKKKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7790..8269
                     /locus_tag="I9573_001063"
     CDS             7790..8269
                     /locus_tag="I9573_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HAT4795691.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            8334..9641
                     /locus_tag="I9573_001064"
     CDS             8334..9641
                     /locus_tag="I9573_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAT4795692.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETDMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9952..10092
                     /locus_tag="I9573_001065"
     CDS             9952..10092
                     /locus_tag="I9573_001065"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HAT4795693.1"
                     /translation="MNGEGCILSNKITKECYKTGYMIREKSIDTYPDSGRGFMTWDEN
                     GE"
     gene            10604..11698
                     /locus_tag="I9573_001066"
     CDS             10604..11698
                     /locus_tag="I9573_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4795694.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQTQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSLLSENKEYKFLIEKLSLLEE"
     gene            11703..11903
                     /locus_tag="I9573_001067"
     CDS             11703..11903
                     /locus_tag="I9573_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HAT4795695.1"
                     /translation="MENLMEYLPLLIPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11903..12820
                     /locus_tag="I9573_001068"
     CDS             11903..12820
                     /locus_tag="I9573_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4795696.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIKIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNNEIQKFKSSGGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12817..13575
                     /locus_tag="I9573_001069"
     CDS             12817..13575
                     /locus_tag="I9573_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4795697.1"
                     /translation="MREYIAFTKKEFKENIRNYKLLSLTILFLVFGIMSPLSAKFMPD
                     LIAHFAPTLKVTTAPTALDSWTQFSGNISGLGTSLTLIVFCNILSNEYSKGTLVIMLT
                     KGLSRSSVVLSKFSIAVIIMTIGFWLSFLCTYGYTMYFWPTANLNHIIFSAFNLWLIS
                     IMYISILILGCVLFRPAFASVLLVLAATAILSLISIPKQIAPYTPNFILSKNIDLVSG
                     KVTPPEFIIPIIVTIVISIVCLLLAVVLFNKKAV"
     gene            13958..15031
                     /locus_tag="I9573_001070"
     CDS             13958..15031
                     /locus_tag="I9573_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HAT4795698.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            15101..15364
                     /locus_tag="I9573_001071"
     CDS             15101..15364
                     /locus_tag="I9573_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795699.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            15570..16547
                     /gene="bioB"
                     /locus_tag="I9573_001072"
     CDS             15570..16547
                     /gene="bioB"
                     /locus_tag="I9573_001072"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAT4795700.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMVFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            16775..17788
                     /locus_tag="I9573_001073"
     CDS             16775..17788
                     /locus_tag="I9573_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAT4795701.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNSAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            17830..18720
                     /gene="rbsK"
                     /locus_tag="I9573_001074"
     CDS             17830..18720
                     /gene="rbsK"
                     /locus_tag="I9573_001074"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAT4795702.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKKLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            18768..19730
                     /locus_tag="I9573_001075"
     CDS             18768..19730
                     /locus_tag="I9573_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAT4795703.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            19784..21295
                     /locus_tag="I9573_001076"
     CDS             19784..21295
                     /locus_tag="I9573_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAT4795704.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            21279..22277
                     /locus_tag="I9573_001077"
     CDS             21279..22277
                     /locus_tag="I9573_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAT4795705.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            22461..23723
                     /locus_tag="I9573_001078"
     CDS             22461..23723
                     /locus_tag="I9573_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAT4795706.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFQMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            23743..24486
                     /locus_tag="I9573_001079"
     CDS             23743..24486
                     /locus_tag="I9573_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAT4795707.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            24659..26044
                     /locus_tag="I9573_001080"
     CDS             24659..26044
                     /locus_tag="I9573_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4795708.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFEYTPII"
BASE COUNT        10331 a         2705 c         4965 g         8271 t
ORIGIN      
        1 ttaatagata agatataatt ttaaaaggag tgatataata tgagtaaata tagttcttta
       61 tgggaatatg ttaagaaaaa taatagtcaa tcgtttaaat taacttttga agaaataaaa
      121 gatattgctg gaatagagat agaccattcg tttttgaaat ataaaaaaga attaaatgaa
      181 tatggatatc aagtaggaaa aatctcttta aaagagaaga ctgttatttt caataaaatt
      241 gattaagata taaataatta catataataa aataatttgt attgacgatt attacaaata
      301 aaaataaagt aaatcttata taagatttac tttattttta gatgatattt ttttagcttt
      361 atactataag gtatataaaa ctaagcctat taaaaatgtt ataatttcta ttaccatcat
      421 agaccatatc aatccatcaa gttcataaaa gtaattaagc tttattaaat agcttcttta
      481 ttataatttc tttatttcat ataatataaa tagtattaaa atttttatgg gagttaaagt
      541 aagcatgtat tattacttag atatttgtat aatcaacatc tagtaattta cctttttggt
      601 gaaaatgatt aataagtttt ttaggataat tcattattaa ttcatctgga aaattaattt
      661 cctttaagat attctcagaa tactcatagt tacctatgtc aaaacaaatg tgagcatctg
      721 aagttagtat gattttagca ccatattttt tgcaaagtgt agcaatatac ttacagttat
      781 ccgagctacc atttcttgac acgcctttaa tagaacaatt atttatttct attaagatat
      841 tcttttctat agctaattta atgattaatt catagtcgat tgggtagttt gggtttccaa
      901 ggtgtcctaa tatttcaata ttatcatgct tatttattgc atttattaaa gctttagtat
      961 tattctctaa tgtatttgga gaaaaaacag cttcgtgaaa tgacaagata agataatcta
     1021 accgaggaat tacaaaaggc tctaggtcta tgtttccttt aatatctagt atatttgctt
     1081 cacagcctct taaaattata atgttattta tgattcttgg gatattttga atgttgtgaa
     1141 agtaccatct gtggggagaa ccaggcatct taggaccatg ttcagatgta cctagtatct
     1201 taattccatt ttctttacaa taattaatat tttctagtaa agtgctatag ccatgaccac
     1261 tgactataga gtgggtgtgt aaatctgata aaaaattcat ataaattcct cctctataaa
     1321 agatgagggt aacgtaattt atattataaa acgaatagga aagaaaatca atagttacat
     1381 aattagacaa aaatattaaa atattgtaga attaagctta ataataaatg tttaacagta
     1441 ttactgctta taggggaggt taactatgac acaaaagaag ctatcaagga aagaaaaagt
     1501 tttaatttgc ttgaaaaggt taacagaaga acacttgaac tttaataatg gcattagatt
     1561 tggatttgat gcagaatata tagctactga agtaggtata aatagaagta atgctagtaa
     1621 ggaattaaat caattaataa aagaaagtat tattataaag ataaagggta agcctgttca
     1681 gtacttatat aaagaaagta ttgagagtat tttgggaaag aaactaaata aagggatatt
     1741 tgataatatt aactctataa acgaatatag tgaaaatgat tctatcaaag atgagtatat
     1801 tttagagagt ataaaaaaag agaacaatat ttttaattta gtaggatgtg atggaagttt
     1861 aaggtcccaa atagaacagg ctaaagcagc agtaatatat ccacctaagg ggttgagtac
     1921 attaataata ggacctacag gagttggtaa atctatattt gcagaatata tgtataagta
     1981 tagtttaact ataaaagaag atagtgataa tgcaccattt atcatattta actgtgctga
     2041 ttattctgat aatcagcaat tattattagc gcagcttttt ggatatgtaa aaggagcatt
     2101 tactggagct gataaagata agtatggtat tgtacatgaa gctaataatg gagtattgtt
     2161 tttagatgaa gttcatagat tatctgcaga gggacaagaa atgctatttc tgcttatgga
     2221 taaagggata tatagaaggt taggagaggc taataaggta cacgagtgta gagttatgat
     2281 aattgcagcc actacagaag acccagaaac agcaatgttg gaaacgtttt taagaagaat
     2341 acctgtaact ataaagattc cgtctttaga ggaaagaggc tttcaagaga gaatgaagct
     2401 tatatgttat ttttttaaag aagagagtgt taggattggt gttaagttaa aggtttcaaa
     2461 agaagttatt aaggcattta ttttatataa gtgtaaggga aacataggcc aattaaaaag
     2521 cgatatacaa ttaatttgtg caagagcttt tttagattat atgacctata aaagggaatg
     2581 tgtatatgta aggctctctc ttctaccaaa taatattaga gaatcattat atggaaataa
     2641 tagaaaagag gagtttgtac aaaatttcag ttttattgga aaagatgata ttattttttt
     2701 accagaagaa aaggattttg aaagtgaaaa tttattaata gaaaataaaa aatatgattt
     2761 agatttttat ggaatgataa aagaaacttg gaataaactt caacaagaag gtattaaaga
     2821 gtcacaaatt agaagtgtta tagatggaga catacagaag tattcctata atttaatgaa
     2881 tacttttata tataataata gtaatcaaac agcttataac aacattgtta atgattcaat
     2941 tgcaactact gtaaaatata ttttacaaaa gcatgataaa tggagcaaaa gagaaggatt
     3001 agataaatta ataaaagcta tagccttaca tattcagaat ttaattgaaa gaataaagat
     3061 tggaaatata gtaaagcatc ctaataaaga tgaaattatg aaggaaagag catatgaata
     3121 taacatagca aaagaaatat tatctaacat gtcattaatg tatggagtag agtttccaga
     3181 agatgaagca atttttttag caacatttct ttatttatcg tatgtgggat taaatgagga
     3241 gagtatagca atattagtca taatgcatgg tgaatctaca gcatctagca tggtgaatgt
     3301 agccaatact ttactggatt gtaaccatgc agtggcaatt aatatgggat tagaagatag
     3361 agttcaggat attttagtac aagctgtaga aatagccaat aaaattgata aaggaaaggg
     3421 aattttaatt ttaactgata tggggtctat tttaacattt gctaaagtta ttcgtgatgc
     3481 tacaggaaaa gaagtaaaag cagtagatat ggtaagtaca cctattgtaa tagaagcaac
     3541 tagaaaggct ctaactccag aaatgacatt agagaaatta tattttaata ttatagaatc
     3601 tataaagaaa cattatggag atatggaagt tacatatgct gaaaaagata atggtggtag
     3661 atattttgat agactactaa ttgataatat tagtaaaact ttgacttttt taaatgggga
     3721 aaaggcttat tttatattaa aagaagtaat taatagaatt agtgaacact attctttagt
     3781 aattcaagat gaacttttag taaagtttat atttcattgt tcatgtatga ttgaaagagt
     3841 aataataaaa gagtctttag tttataaatc atatgaagaa agaattagta gaaataaaga
     3901 attatattta gttataaaag aaagttttaa gctagtagaa gagacattcg atataattat
     3961 ttcagatatg gaatatgcaa atatattaga tatatttgaa tcacaatatg atacagataa
     4021 attcaaaagt tgatacaaaa tttaaaaata gccaaaaggc tatctttttt ttactttttt
     4081 tgatacagat ttgatacagg ttgaaaaaat cttttgatac atatcgaaat aataagtttt
     4141 taaaaaaaat tgagtaaaaa gttttgatta aatcctgtaa aatagccatt tattattgat
     4201 acacataagt tggcacggca attgcttata taaaaagtga caagaaaagt gaggtgaaat
     4261 tggtgatagg aataatagtt atgactcatg ggagttttag tgaagaaatt ataaaaagct
     4321 gtgaacttat tgcaggacca gcagaaagaa cagcagcaat taaactaaat aggaatgata
     4381 atattgaaga cttaaataaa aattttgtag aaaagttaaa tgaattagat gaaggtgacg
     4441 gagttttagt attagctgac ctattaggag gttcgccaag taatgttgca agtttaaacc
     4501 ttaaaaaagg tggcaagttt catgcactta ctggagtaaa tcttccaatg ctacttgaag
     4561 ctttaattaa tagagaggga aaaagtttag aagagttagc agaagcatgt attgaagcag
     4621 gacaaactgg tataaacaat attaataaag ttcttagctc aatgtaatag aaaaaggagt
     4681 ttgattaaaa atgtctttag taaaatattg cagatgtgat gatagattaa tacatggtca
     4741 agtgatatat aaatgggtta agcatctagg tgtaaaaaag atagtagtag tagatgatga
     4801 aacaaccaat gatgttattg ctaaaggttt gataaaaatg gcagcaccaa agaatattga
     4861 tttatcaata ttaactgtaa gtgaatcaag gagatatttt tataataatc aagctgatga
     4921 taacgttttt gtattaatta aaaatttaga tactgcaaat agaatgatag aagaagggat
     4981 taatatcaaa aaacttattg ttggaagaat acctacagga ataggaaaaa agaaaatatc
     5041 tcaaaatgta tacattaata aaaaagagtt tcttttaata gatgaattta ttaaaaagaa
     5101 tattaatgta tcaattcaaa tggtacctga tgaagaagaa gtagaattaa accaaaatat
     5161 taatcgctat aaaggagagt ttatttagca atgaataaat ataaggtaat tatactgtgc
     5221 catggaactt ggggaaaagt cttagtagaa gaaacaaata aaaactttgg attgtcaaat
     5281 caatatgaag ttttatcact aagttctgaa aaagatgttt ctgtatttat gaaagaggtt
     5341 gaagaagtag tagataaaga agatgtgaaa attataatat cagatttata tggaggatct
     5401 acatcaagtg ttgcaatagc tgttgctata agaagaggaa tagatgcaat aaatggactt
     5461 agcttacaga caattttaat cgtagatgaa gaattaagca aagaagataa attacataca
     5521 ttaccagaaa gaatcgtcaa aaaaaataat aacctttgtg tggatttaat taaagaattt
     5581 aaaaaaatat aaattatgag gtgaatataa tggcaaaaat aacattagta agagtagacc
     5641 acagattaat tcatgggcaa gttataacaa aatgggtaaa aatcgctcaa gctcaaaaaa
     5701 taataatagt tgatgacttc ttaggtcaag atgaatttat ggctgacata tataaaatgg
     5761 cagcaccatc aggagtagaa gtagttattt taacagcaga agatgcggga caagcattcc
     5821 aaaacaacac tttaggagat aaaaatatat ttattctatt taaaaatgtt gatatggcaa
     5881 ataaggctta taaagctgga cttaaatatg aaaagattca attaggaggt attccaaatg
     5941 aagcaggaaa gaaaatggtg tttacagctg tttcattagg aaatgaggat gtagaacaac
     6001 ttaatgaact taatgaaaat ggggttgaaa tagtcctaca agttatacca gaggaatcat
     6061 ctatgacata tgaaaatgcg ttaaagaaat ttaaataaaa aatataaatt aggaggaatt
     6121 ataaatggaa tctgttttga tgttggcaat tgtaacagga ttatggtatt ggtttgcagc
     6181 aggtcttgca gggtataccc ttttctcaac actaaagtct cctttattta taggttttag
     6241 ccttggatta ctttggggtg atgtaacaac tggtatgatt gttggtgcaa gtatagaaat
     6301 ggtttatcta ggaatggtcg cagcaggggg aaatattcca tcagataaat gtctagctgc
     6361 tcttattgca ataccagtag cacttcaaac tggtgttaat gcagaagttg cagtatctat
     6421 tgcggtacct ttaggtgtta taggagtatt agttaataac ttaagaagaa ctggtaatgc
     6481 tgtattggtt cataaggcag ataagtatgc tgaagaaggt aatacaaaag gcatctggag
     6541 atgtgcaaca ctttattctt taatatttgg gtttgtttta agatttccaa tagtttttgt
     6601 atgtaacttt tttggagcag atttagttca atcattatta gatgttatac cacaatggtt
     6661 aatgaatgga ttaacagtaa tgggcggtat attacctgca ttaggttttg caacaacaat
     6721 atttactata ggtaaaaata aattcttacc aatgtttatt ataggatttt ttatggttca
     6781 atattttgaa atatcaatta ctgcagctgc aatatttggt gtttgtatag ctcttttaat
     6841 tacctttatg aaagaagaca aaagggtagg tgaagtataa tgcaagaaat aaaaaataaa
     6901 gaagttaaaa atggacaagg tccaactgaa gttaataaga caagagtact cacaaagaaa
     6961 gatgtaacaa aaacatacct aagatggtgg tggacagcag agttatctaa ttcttttgaa
     7021 agaatgcaag cattagcagt ttgtgcaagt ttcactccgg ctttagaaaa gttatataag
     7081 aaaaaagaag atttagtaga tgcattaaag agacacttac aattttttaa tactcaagct
     7141 atatggggag gattaattca tggtacagtt ttagctatgg aagaagagaa agctactgaa
     7201 ggaaaaatcc caggagaagt tatttcaggt gttaagaatg gtcttatggg acctttggct
     7261 ggaattggag atactttaga ttttggtaca tttcaaacaa tattcttagc tttaggtgct
     7321 tcatttggag cagaaggaag tgttataggt gcatttttcc caataatgtt tagtatatta
     7381 ctattttgcg aaggttatta cttatttcat ttaggatatt ctttaggtag agattcaatt
     7441 aagaaaattc tatcaggtgg tattgtcaat aagataattg atggtgcttc aattctagga
     7501 atgtttatga tgggagcatt atctgcaact acagttaagc tttcaactcc gctatcattt
     7561 gatattggtg gtaaagcaat tgttgttcaa gatacattaa atatgattgc tccaggttta
     7621 ttaccacttg gtgttgtatt cttcgtatat tggggaatga aatacaagaa gtggacaata
     7681 actaagttat tagtaatttt agttgtatta gcattggtag gatcattcat tggaatattc
     7741 taattcacag tgattaaaat atagaattta attttaggag gagaaagaaa tgtcagaaaa
     7801 aatgtataca gaaaaggaat atttagaagg aattagagga gcaacaggag aaagagcagt
     7861 atggttctat cttcttatga aagaagctga aaaattagga gctaatccag atgatatatg
     7921 taaagaagca atatatggat ttggtaaaat gagaggtcaa aaatataacg tagcagatac
     7981 tccaggaaaa atggcagaaa tgttatataa cagtaaagga caaaaagtgt ttgaaatgga
     8041 attagtagaa aatacagatg agaatggcgt tttaaaattc catcactgtc cattagatgc
     8101 tgcttggaaa gaatatggat taactaaaga agaaagaaaa gaaatttgta gattagcttg
     8161 ttatggggat tatggaagag ttgactgtgc acaaggtgtt aaattagatt ttgctcaaaa
     8221 atgtgctcat gatgatgaag tttgtgaatt agtatttaca aaaaaataat ttttaatgct
     8281 aaaagcctgt tctttaagga caggctttaa aaagatagaa agtaggttga tttatggata
     8341 taagcagaag tgtagataat agtttaaata gaaccataga atttctaaaa gaattgataa
     8401 aaattgattc acaacaaggt gaacctattt cacaatgtcc ttttggtata ggacctaaaa
     8461 aatctttaga taaaacttta gattactgtg ctagtttagg ttttagtgtt aaaaatattg
     8521 ataattatat tggatatgca gaaattggag agggagagga attaataggg attccaatgc
     8581 atttagatat agttccacca ggagaggggt ggagtgtaga cccattttcg ggagcagtaa
     8641 ttgacaatat tatttatgga agaggtgtca tagataataa gggtgctgta tctatgctta
     8701 tacatgtatt gaaaaacata gaggatatgt atcctacaat taataaaaga ataagattaa
     8761 tttttggtac aaatgaggag acagatatga aatgtatcaa atattactta gataaaggtg
     8821 aagaaattcc aagtatggga tttactccag atgctatgta tccagtggtt aatggagaaa
     8881 aaggaagggt tcatataagg atagaaaaag aaattaaaat tgacaaaagt aaaccttaca
     8941 taatagtaag gggaggaact aaagaaaatg ttgttccatc tcattgtact gcaaaaatta
     9001 ttaatggaat tatttcagag ttaactacta aaggagtagc tgtacatgca agtaatcctg
     9061 aaaagggaga aaatgctata tctaaaatgg taataaagat agttgaggat aatatggatt
     9121 ttcagcatag agaagatata gaattagttt caaaatacct ttgtagtgat tattatggag
     9181 atgcattggg gattaatcaa tatgatgagg tattcaaaaa cactacctta aatttaggaa
     9241 tattaaaagt aaatgaggaa aaaatcgtat gtgagttaga tataaggtat ggtaaaaata
     9301 tagttttaaa taacataata gatagattca aaaaggtttt ttgtaatggt tggaaaatag
     9361 aggttattgc tcataaagac ttacattatg tggatgagag taatttagta cttaaaaaat
     9421 tattagaagc ctatgaagaa gtaacagatg aaaatggata tacaattgct atgggtggtg
     9481 gcacatatgc aagttggttt aaagatatgg tggcgtttgg acctaagttc ttagcgtata
     9541 aaactggtgg tcatggagta gatgaaagag taccaataaa tcatatacga aagaatatgg
     9601 agatatatac tttggcttta ataaaactat tagaattgta aaagaagaag agactttatg
     9661 tgcaaattta cagatatctt aaaaagagag ataaaacgag ttcaacttta tttaaatgtt
     9721 caacaccatc aaatggggct gtctcaaaat agagataagc ctcttgctta ttattgaaat
     9781 gatgaaaata tattttactt catacaaaaa agagtgcctt atgaagtaaa aagttaattt
     9841 tgagacaccc tcatttgatg atgttgaaga gaaaataagt atgaaattat taaaaaaaga
     9901 tgattttata aaagttaagg ttgaaaataa aagttaattg aatggaatta gataaatgga
     9961 gaaggctgta tactatctaa taaaataaca aaagagtgtt ataaaactgg ttatatgatt
    10021 agagaaaagt ctattgatac ttatccagat agtggacggg gatttatgac ttgggatgaa
    10081 aatggagaat aataaaaatt atagtttata caatttaata agatatatgt acaagcagtt
    10141 aatctaaatg attgactgct tttcttttat agatttttat ataatagaca attactaata
    10201 catgatagtt gcctgttata attagaggaa tagatacgaa taataacttt ataaaagaat
    10261 taaatggaaa attgaagagt tatattcagg attttaaaaa gaatatgaaa tgagaaatga
    10321 aaataaggtg tttatattaa aagaggtttt acacaaaata cttaaaaacg tgataaataa
    10381 aaactgtatt tagataaatt aactactagt tttatatata atatggattt tgtttacaaa
    10441 gtttacatat ttcacaaaat atttttaaaa tatatttaaa aatatatttt atatacttgt
    10501 tcgtttgttt ctctgtggtt gattttttcg tcaaaagtca acttcaaatc gcttgttagc
    10561 tttacatgtt taaggatata attatacata aggagggatt gacatgaatg aattaaacat
    10621 cgcaaaaacg ctgattttaa agcgtaaaga aaaaggaatt acacaagatg aacttgcaaa
    10681 ttatataggc gtttctaaag cttctgtatc taaatgggaa actggacaaa gctatcctga
    10741 cataaccttt ttaccgcaac tcgcgacata tttcaacatt accgttgatg agttaatatg
    10801 ttatgaacct caaatgatga aggaagatat taataaactg tataataaac tgtgcaaaga
    10861 ctttacggtt aaaccgtttg atgaagtcat ggatgaaata agagaaatca taaaaaaata
    10921 ttattcatgt tttcctttga tttttcggat gggtctttta atagttaatc actatgatat
    10981 tgtagatgaa aaaaagcgtg aactattaat tagtgaagca ttagaaatat ttattcgtat
    11041 acaggaaact tgtaatgaca tagatatttg ccgtcaagca aaaagtatgg aggcaacttg
    11101 ttatatattg ttaaatcaac ccatacaagt gattgattta ttgcaaaaca gcaattttcc
    11161 catgataaat gaatctatac ttcttgcaca ggggcaaatg atgaatggtc aaatggatga
    11221 agcaagagaa acatttcaac ttggtgcata tcagaacctt atatcattag tccaaaatct
    11281 agtgggtatt ttgcaaaatg cagataaatt acaaatgaag gaaatagaaa gacgtattct
    11341 agcaatttca gatatatttg agcttgatac attatctcct gctattatgc tttcttcata
    11401 tttaacacag acacaaatta atttaataca tggggataat gaaggagcta ttaaatcatt
    11461 acgaaaatat gtagatttag caacacgtga tatataccca attataatac atggggatga
    11521 cttttttaat aaacttgaca gatggatttc tgaaattgga acaggtatta cacgtgatga
    11581 tacaatcgta aaagcaggta ttgttgctgc tattaaaaat aatccaatgt tttctttatt
    11641 aagtgaaaac aaagaataca aatttcttat agaaaaatta tctttattgg aggaataaga
    11701 ttatggaaaa tttgatggaa tatttaccac ttttgattcc tgttattatt ttagatttaa
    11761 tacttataat tactgcactt gttcatgtat tgagacatcc aaattataaa attggaaata
    11821 aggcaatatg gattattgta gtactgttta ttagtctaat aggaccaatc ttgtatttta
    11881 caataggaag aggtgaagaa tgatgaatat tgtctcaata aatggattat caaaaggttt
    11941 tggtaatcga aagataatag ataatctaaa ttttactgta ccagaagggt cagtctttgg
    12001 gtttgtaggt aagaatggtg caggtaagac tacaacaatg aaaatggtac ttgggttact
    12061 tgaaccagat agtggaacta tagatgtttg tggtgagaaa gtgacatatg gaagaacaag
    12121 ttctaatcgt catgtaggtt atctgcctga cgttccagag ttttacaact atatgagacc
    12181 acttgaatat ctttctcttt gtggtgaaat tacggggctt tcaaaaaaag aaataaagat
    12241 aaggagtgaa gagttgttat cacttgtggg gcttggcaat gaaaagagac gaataggtgg
    12301 gttttctcgt ggaatgaagc aacgacttgg tatagctcaa gctttgcttt cacgtcccaa
    12361 attacttatt tgtgatgaac caacaagtgc acttgaccct gttggtcgaa aagaaatatt
    12421 agatattatg cttaaaatca aagactcaac aactgtaata ttttcaacac atatactatc
    12481 agatgttgaa agaatctgtg accatgttgc tatactaaac aaaggaagca ttgctcttag
    12541 cggtacactt tcagaaataa aaagtatgca cggaaaggat agacttttac tggagtttgc
    12601 aagtaataat gaaattcaaa agtttaagtc tagtggtggc attaaatcct tactaaaaga
    12661 ttcagaagaa actaatatgg aaattgtttt gcatggtaaa gatataaaag caatacaaaa
    12721 aacagttata tctacactcg cagaaatgaa tctttgtcca gtaaagatgg aactaataga
    12781 attgtcactt gaaaatttgt ttttggaggt agtaaagtga gagaatacat agcatttaca
    12841 aaaaaagagt ttaaggagaa cataagaaat tataaattac ttagtttgac tattttattt
    12901 ttagtatttg gaataatgag tcctctttct gcaaaattta tgccagactt gattgctcat
    12961 tttgctccaa ctttaaaagt tactactgca ccaactgctt tagattcttg gacacagttt
    13021 tctggaaata tttctggtct tggaacgagt ttaactctaa ttgttttttg caatatttta
    13081 tcaaacgaat attctaaagg aacactcgtt attatgttga ctaaagggtt atcacgttca
    13141 tctgtagtat tgtctaaatt ttctattgca gtaattatta tgacaattgg tttttggttg
    13201 agctttttat gtacttatgg atatacaatg tatttttggc caactgcaaa tcttaatcat
    13261 attatatttt cagcatttaa cttatggtta attagtatta tgtatatttc catattaatt
    13321 cttgggtgtg tactatttag accagcattt gcaagtgtgt tacttgtatt agcagcaact
    13381 gctattttga gtttgatttc tattcctaaa cagattgcac cttatacgcc taatttcata
    13441 ttatcaaaaa atatagattt agtttcaggt aaagtgacac ctccagaatt tattattcca
    13501 atcattgtaa caattgttat ttcgatagta tgtctgttat tagctgttgt tttgtttaat
    13561 aaaaaagcag tttaaggata tttttattat aaatacaaat agtgctgtat tgtgggaaaa
    13621 tgtgtttagt attgtagaag aatatattta agtaaaaaag aggctgtctc aagatagaga
    13681 aataagcctc ttttttattt tagtcctcct cactatactt tataataatt aagttattac
    13741 cttaaggaac tatctaaaaa aagagatata gattataatc taatgcttat cagttatttg
    13801 aggtgtttag tatatagagt ctaaagtagt gaaggactaa aataatattg ttttcattaa
    13861 ataaagataa gaatctttaa ttgtggtaaa atatactaaa aaatcatttt aaaagtgata
    13921 aaattatttt aagtaaatat tttaaagggg tgtagatatg gaaaagtcaa aagtatattt
    13981 ttgtgattta tactcaaatt cgcaaaataa aaacgtacca aacaatgtaa gaagattatt
    14041 tgatgaagca ggattcaaag atttaataga aaagaatgac caagtcgcaa ttaaacttca
    14101 ctttggagaa aagggaaata ctacatatat gagtcctgtg gcagttagac aagtggttga
    14161 taaggttaaa gactgcgaag gaaagccatt tttaacagat acaaatacac tgtatacagg
    14221 gagtagaaca aattctgtag accatcttac aactgctata gaaaatggat ttgcttatgc
    14281 agttgtaaat gcaccagtaa taattgcaga tggattatat agtagaaatt atgagaatgt
    14341 aaaaatagac aaaaaacatt ttgaaagtgt aaaaataggt ggagaaattt ataattcttc
    14401 tgcaatgata gtaatgagtc attttaaagg tcatgaagca gcaggttttg gaggagcatt
    14461 aaaaaactta gctatgggat gtgcaagtgc agcaggaaaa caaatgcaac attctgatgt
    14521 tactccagta gtaaaggaaa agaagtgtgt aggttgtgga aagtgtgtta attcttgtcc
    14581 tacaaaagct ataagtatag tggataaaaa agcagttata gattctgatg tatgttatgg
    14641 atgtggagaa tgtccaactg tctgtccaac tagagcagta actatacaat gggaaagtga
    14701 ctcagatgtg tttgtcgaaa agatggcaga atacgcttat ggagcagtat caaataaaaa
    14761 agacaaggta gggtatataa cttttgttat gaatgtaact ccactttgtg attgtgtacc
    14821 ttggtcaggt agaccaattg cccatgatat aggaatatta gcatcaacag accctgtagc
    14881 tattgaacaa gcatgctatg atttgatttg taaggaaatg ggtcatgatg ttttcaaaca
    14941 tgaacatcct catgtaaatg gaactagaat aatagactat gcatgtgaaa tgggtatggg
    15001 aagtaaagaa tatgaattaa taaaattata agaatagtag cacttaaatg catataatta
    15061 ttgtaagaga aatctaaaag tatcaaagga gtatgcattt atgaaaattt actatgagaa
    15121 ggaaaacagc aagaaggcta atgtaaacaa tattccaagt gtatttgcgt ttaattttct
    15181 aagaaacttt ttgactagca aagagtatga ggaaactaga aaagaatatt ttataaataa
    15241 tctaacaaaa agtcaggtta atcaagccat gaaggattta aaatggctat ttagagaata
    15301 tgaaggctta gaggttatta caatggaaag cttggatgga gttaagacaa ggatagtttt
    15361 ataaaccagt aagaattata ttctaataaa aaatactgct ttaataataa tatgtataat
    15421 ctatatacta atattatttt gaagcagtat ttttttgtgt atagtaaact tgtgtctaaa
    15481 ataaagttga ccaataaatg ttatatatgc aatactatat ataataagta aactacataa
    15541 aaatattaag ttgacaaata ggaggatata tggaaaaata cataataaaa ttaaaaaata
    15601 aagtattgag agaaaaagaa ataagttatg aagaagctct taatttaata agccttgata
    15661 caaataataa aaacgatttt gatattctat taaaatctgc aaatgaaata agagaatatt
    15721 ttatgggaag aaaagcagat ttatgtacga taatgaatgc taaatctggc aaatgtagtg
    15781 aagactgtaa attttgtgct caatcatctc attataaaac tggtgtagaa gaatattcat
    15841 tattagatta caatgaaatc ttaaatagag ctaaagagat ggagagtaaa ggagtgcata
    15901 gattctcatt ggttacaagt ggtaaaggta tgtcaggaaa ggagtttaat aatattttaa
    15961 atatctatga aggattgagg gaaaatacta atttaaaatt atgtgcatct ttaggtatta
    16021 tagattatga gcaagctaaa atgttgaagt cagcaggtgt gactacatat caccacaatg
    16081 ttgagacgtg tagagataat tttcataata tatgtacaac acacacatat aaagatagga
    16141 taaaaactat aaaagatgct aaaaaagctg ggctagatgt atgtgtaggc ggtataattg
    16201 gaatgaatga gagtgaagaa cagagactaa aaatggtttt tgaaatacga gaattaaatg
    16261 taaaatcatt tccaataaac attttaaatc ctattaagaa tacgccaatg gaaaattatg
    16321 atgtattaga accaatggaa atactaaaaa caacagcagt atttaggttt ataattccta
    16381 atgtgtacat aaggtatgct ggtggaaggc tatcattaaa gggatatgac aaagttggat
    16441 ttaatggggg agtaaattct gcaattgttg gtgattattt gacaactgtt ggaagtggga
    16501 ttgaaaatga taaaaaaatg attatagagc aaggatttga actttaataa atactactta
    16561 aattaattaa tatactttta gataattata aaagtatatt aatttttttt gcagattaat
    16621 ttaaatatta aaattgaatt ataagttgca atattttgaa aaaagggaaa aatatcaaaa
    16681 tttatataaa atggttgcaa atgggaaaag gatgtgatag tatctaggta accggttaag
    16741 taaaacggtt tcctaaattt tgggggtgaa cttcttgagt ataactataa aagaaattgg
    16801 cgaattggct ggtgtttcta aaacaactgt gtcaaaagtc attaataata aagatgaaaa
    16861 tataagtcag gcaacaagag aaaaaatttt gaaaataatg aaggaaaaaa attatgtacc
    16921 aaataaatta gcacaaagtt tagtaactaa aaaaactaat actatagggc ttcttatacc
    16981 agatatacga aatccattct ttacagatgt atcaagaggt gtagaagaca aagctaatga
    17041 agaaggctac aatataattt tatgtaatac agatgaagat gctaaaaaag aatatgaagg
    17101 aataagaaca ctatcagaga ggatgataga tggaattata tttgcagcat cttcaaatac
    17161 aaactggaaa gaagcaaatt ataaagatat aaaaatacca actgtcttga ttgataagaa
    17221 gataagtatg aacaaagaaa ccttaaaagg aattgttaaa ataaataact ttgaaggggc
    17281 ctatatagct acaaaacatt tattagatat tggaaataag aaaataatat acctaagtgg
    17341 tccacttcaa aatgaaatag cagtagatag attagaagga tataaaaagg ctctaatcga
    17401 gtgtaattta agctacaatt ctgcttatgt atttgaagga aaatataaga ttgaatgggg
    17461 acaagaattt ataaaaaatt tagaaaagat agattttgat gcaatatttt gtgcaaatga
    17521 tttgattgca ataggtgtca taagaggtct taaagaaaga gggttaagta taccaaatga
    17581 tgtaagtgtc gtgggatttg atgatattca aacatcaagt ctaataagcc cttctcttac
    17641 cacagtaaaa cagccgtctt atgatatagg atataaagcc agtgaaatat tgataaattg
    17701 tttaagagga gataaaaaag aatcatttga tgaattgatt ttcaaaccag aattagtcat
    17761 aagagattca acaaaagaaa gtgaataaca gcacgagaga cataaataga tatgtaaagg
    17821 agtaatgtaa tgggaaacat agttgtcata ggaagtgtaa acatggacat ggtatgttct
    17881 gtagataaaa gaccagaaaa aggagaaaca gtattaggta atagtttttt tacatcacct
    17941 ggagggaaag gggctaatca agctatctca gcttctaaac taggagcaaa tgtaaaaatg
    18001 atatcatgca taggggaaga tggcttagga gaggagttaa taagaaattt tagaacgaat
    18061 aaagttgatt atagtttagt atccagaaat aatcacaaaa gttctggtgt tgctgttata
    18121 acattatgtg aaaatgataa tagtattgtt gttgtaccag ggacgaatga gctagtagat
    18181 atagaattaa ttaaaaagaa tgaagaagag ataaagaatg cagatatagt attgctacaa
    18241 ttagagattc cattaaaaac aataaattat gtagtgaatt tctgttttga aaataggatt
    18301 aaggttttat taaatccagc accagcagta aaactaaatg aagatataat agaaaaagta
    18361 acttacttaa caccaaatga acatgaatat aagatagttt ttgacacaaa tgaagggata
    18421 gaagaagtat taaaaaaata tccaaataaa cttgtaataa cagaaggaaa aaatggagct
    18481 agattttatg atggtgagga aatcaagcat gtatcttgta taagtgttga tgttcaagat
    18541 accacaggag caggagatac atttaatgga gcattggcag tggctataac agaagggaaa
    18601 aaattatata cagcagtaga atatgcagta gtagtatcag gtctttctgt aactaaacta
    18661 ggtgcacaat ctggtatgcc ttataaagaa gatgttgaaa aatatttaaa taataaataa
    18721 aaatctaatt tgttcaaatt taaaactatt aaaaatggag gttaattatg agagtgagaa
    18781 aaattttatc tttaggaatt gctggaatac tagcaatagg aatgttaaca gggtgttcaa
    18841 tggaaggacc aagtaaatca gataataaag gtggttcaga caagaaagat ttaactattg
    18901 gagtatctac aataactctt caacatcaat tttttataga tattgatgag ggaataaaag
    18961 aaaaagctaa ggaattaggt gtgaaagtta tagtaaatga cccagaccaa gatgtagcta
    19021 agcaaacatc agcaatagaa gattttatac aacaaaatgt agatggaatg atagtacttg
    19081 gaactgataa ttcggctata gtaccagcag tagaaggagc atttgaaaaa atgccagtag
    19141 ttacagtaga tgctgtatta aatactgaaa atattacaag ttatgtagga acagttagtt
    19201 atgatgctgg taaaaaatta ggggaataca ctaaaaaata catagatgaa aagttaggtg
    19261 gaaagtcaga aatagcaata gtgacagatt taaaatctca aatacaaatg cagagaatag
    19321 atggatttaa agatgcgtta aaaggttcag caaatgttaa gatattaaat tcacaaccag
    19381 gatatgacag agaagaatct cttaatacag tagaaaactt aattcaatct aatccagatg
    19441 tagatataat atatgcaaca gctgaaaata gtgtactagg agctaaagct gcacttgaat
    19501 ctgcaaaaaa taaggatgtt aagattgtag gatttgactt aactgaagaa gcatcaagtg
    19561 gaataacaga tggaactatt cttgctatga ttcaacaaca acctaaagag atgggacgtt
    19621 tagctgtaga agcagtagta aaagctataa aaggggaaaa ggtagaaaag aatataccag
    19681 taccagcttt actttatgat aaggaaaata taaaagactt taagaattag taaaataaaa
    19741 tgagattatt tgaaagaaat cctatttaaa aaggagtgtc attatgagta atataattct
    19801 gaagttaagt aatattgcta aagaatttcc tggtgtcaga gcattagata atgttaattt
    19861 tgagttattt catggcgaag ttcatgcact actgggtgaa aatggtgctg gtaaatcaac
    19921 aatgataaag attttaactg gtgcacattc gaaaacttct ggaaaattta tttttgaagg
    19981 caaagaaata gagaatatat ccccagatat ctctaaaaag ataggtataa atgccatata
    20041 tcaagaacta acagtttttg acgaattaac agtagcacaa aatattttta tgggaaaaga
    20101 aataaatggc aaagtcttaa ccaatgacaa aaaaatgaat gaagaggcta aaaagatttt
    20161 cgataatatg ggaatagata taaatccaaa tagtttagtc aaagagctta gtatagctca
    20221 aaaacaaatg gtagaaatag caagagtttt atcatcagaa actaaagtat taataatgga
    20281 tgaacctact tcttcaataa gtaaaaaaga gactgaaata ctttttagac ttataaatga
    20341 tttaaaagag agtggagtta gtataatata tatatcccat aggatggaag aactctttga
    20401 gatttgtgac agaataacta taatgcgaga tgggaaaacg atttcgactt tgaataccaa
    20461 agatgtatcg tctgaggaag aattagttaa tttaatgata gatagaaagc tagaccaatt
    20521 tttcccaaaa agaaaagtag aaataaaaga agaaattatg cgagtggaaa atctaactaa
    20581 gaacaatgtt tttaatgata ttagctttaa tataagaaaa ggtgaaatat tgggaatagg
    20641 tggactagtt ggaagtaaga gaagtgaaat agtagaagct atatttggac ttagaacata
    20701 tgattctggg aaaatatatt taaacaatga agaagtaaag tttaaaactc catcagatgc
    20761 aatagaaaat ggactgggat taataacaga agatagaaaa ggtactggat tgtttttaca
    20821 aatgagtgtc aaagaaaaca caactatggc tgggttaaaa aagatatcta agtttaaatc
    20881 aattatagat agaaaaaagg aaaaagagat tttagaaaaa tacatagaag ctttaaagat
    20941 aaaaacacca catatgaatc aagttataca aagtttaagt ggtggaaacc aacaaaaagc
    21001 tataatagca agatggcttt tattacaacc agatatattg attatggatg aaccaaccag
    21061 aggtatagat gtaaatgcta aggcagagat atacaatcta atgggagatt tagtggagag
    21121 tggtgtaagt ataataatga tatcttctga aataccagaa ttaatatcaa tgagtgacag
    21181 aataatggtt atgagagaag gtcatataag tggattttta gaaggtgaag aaatggttga
    21241 aaataatgta ttaaaattgg ctttcggagg taaaattaat gagttcaata actaagcata
    21301 aagaagaaaa atcttctcca ttattgacaa aattaacaag tgtatttaga gaccaaggga
    21361 gtgcagctgt aggacttgta ataattttta taataatgag tgtagcttca tctaactttt
    21421 taactttaga taatttaatt aatgttggaa gacaaatatc aataaatgca atacttgcag
    21481 taggtatgac atttgtaata attacaggag gaatagattt aagtgtagga gcagtaattg
    21541 ctttagtagg tacattttgg gctacaacag tagtgaatta taatgcacct atatgggtag
    21601 gtatgatact tgcattagca atagggacaa tattaggagt cataaaaggt gcaattattt
    21661 caacacaaaa actacctcca ttcattgtaa ctttagccat gttaacgata ataagtgggg
    21721 caagctttgt atttacagga ggaagaccta tatcagtaaa tacagatgca tttaagatgc
    21781 tcggtagagg atatattgga ccaataccaa taccagttat aattatgatt atagttgtaa
    21841 tagcaggaca ttttctatta aaaagaacta attttggacg tcatgtacat gcagtaggtg
    21901 gaaatgaaga ggccgctaga ttatgtggag taaaagtaaa taaagttata gtaaaagtat
    21961 acgcactagc tggactacta acagcattag ctgggataat actatcatca cgtttggcat
    22021 caggttcacc aactgttgga gatggagcag aattggatgc tatagcagcc gttgtgcttg
    22081 gtggtactaa tatgatgggt ggttctggtt ctatagtagg tacatgtata ggtgtcggaa
    22141 taataggaat attaggaaat ggattaaact tattaagtgt atcttcatat aatcaaatga
    22201 ttataaaagg tttggtaatg ttatttgcta tatggataaa taatataaaa ttaaaaaaat
    22261 cagctaaatc taaatagtaa ttaaatttta gcttataaac tataaaaatt attaagtttt
    22321 tgtatatatt tttatacttt aatttgcata catctttata cattgaatgg gaatatgcta
    22381 agtgaaaaag tttactatgg cttaaataga tgagattaga cttgaaattt taaatgtaaa
    22441 aataaatctt aggaggaaat atgaaaaaaa ttctcttaga tacattaaat agtaaaaaac
    22501 aagaatacat agattattta aaagaacttg taagtataaa gactgaagat gttggtcatg
    22561 gaatactagg tggatttgaa aaagaaggac aggaatatat tgaaaaactt gcaaattaca
    22621 taggttttag tgtagataga caagaaatga gcgaagaatt aattaaaaaa gcaaaaaata
    22681 tatataaaga aggaaatcta ggtcacaatt atcaagatag atataattta atttgtaagt
    22741 atagtgatga tttacctggt aagacaattg tatttaatgg tcatgtagac actatgccac
    22801 caggtgatat aagtaagtgg aaatataatc catatcgtgc tacagaagat aatggaaaac
    22861 tttatggtct tggaactgct gatatgaaat ctggacttat agcatcaata ttagctgtaa
    22921 agcttataaa agatagtggt ctaaatgtac ctggtaatgt aaaaataatg tcagtagtag
    22981 atgaagaagg tggcggaaat ggtaccataa atgcagttat gaatggtatt ggtggagatt
    23041 gttgcataat ttgtgaacca tcagaacaaa acttaatagt tgcacatatg ggatttgtat
    23101 tctttgaagt tgaggttaaa ggtgtgtctc ttcactgtgg tagtaagtgg gagggtgtaa
    23161 atgcaattga gaaggctatg ttgttgcttc aagatataaa agagttggaa cataactggc
    23221 ttatgatata taagcatcca ctattacctt cacctacaat aaatttaggt gtaataaatg
    23281 gtggaacagc aggttctact gtgccagata agtgtgtatt taatttatgt gtacacttct
    23341 tgccaaatat aatgagttat gaacaagttg taaatgatgt aacaaatgtc ataatgacaa
    23401 gggctaatgg agatttatgg cttaaagata ataaaccaaa tataaacata tatcaatcag
    23461 ggttaggttt tcagatggac aaggattctg attttgtagt gaatgcacat aaaatactag
    23521 aagaaacttt aggaaaaaaa ttagagataa aaggttcaac agctggcaat gatgcgagat
    23581 taatgaaaaa tttagctgaa ataccaacac ttatattagg gccaggttca atagaacaat
    23641 gtcattcaat tgatgaatat gttgaaatta aggagtattt agatagtata cttatgtatg
    23701 caagtctaat attaaactta taaaagtagg aggaatatat aaatgaaaaa agtattaata
    23761 gtaggggaat cttgggtaaa aaatataact catataaaag gatttgatac gtttgttaca
    23821 actcattatg aagaagctgt aaagtggttg aaagaagcaa tagaatctgg aggatatgag
    23881 acagttcata tgcctgcaca tgtagcagca gatagttttc cgtataaatt agaagagtta
    23941 aatgagtatg actgtataat attatctgat ataggttcta atacgttctt attatctaat
    24001 agtactttta tagattgcaa tagtaatcca gatagacttg aactaatcaa agagtatgta
    24061 aataatggtg gggcgttaat catggtaggt ggatatatgt catttacagg aatagatgca
    24121 aaagctagat ttggagaaac tgctatcaaa gatgtattac caataacaat gatagataaa
    24181 gatgatagag ttgaaaaacc agcaggaata attccagaag ttattgactc tgaacatcca
    24241 gtattaaaag gtataccaac agaatggcca aagtttttag gatataacaa aacagtagct
    24301 agagataatt gtcctgtttt agcaactatt ggaggagacc catttgtagc tgttggcgag
    24361 tttggaaaag gaaaatctgc tatctttagt tctgactgtg cacctcactg gggtccaaaa
    24421 gaatttactg attggaaata ctataataaa ttatgggtta atatgcttga ttggttaaca
    24481 tgctaaaaat tttataaaac ttgaaaacct cttacttgat aaaatgaaac ttacaagtca
    24541 agaggtttta ttgtataaaa tggttgtatg gtagaaaata tttttaaatc agttaaaatt
    24601 agtcattttg gctgataatc gataagttat tagaaacagg gaggtttata taattaatat
    24661 ggataattta aataaaaagt ttttgatgga atatatagat gagatacagg gagaattaag
    24721 agatatatca gataagatat ggaaaaatcc agaacttcag tataaagaat actatgcttc
    24781 aaatttacaa aaagaatgtt taaaaaaaca tggttttagt atagagggat tagaaggtat
    24841 ggatacagca tttgtggcta gttatggaga aggtagacca gttatagcag ttttgggaga
    24901 atatgatgct ttagatggtt tgtctcaaaa agtatccact actaaagaac ctattgtaga
    24961 tggtggtgca ggtcatggat gtggacataa cttacttggt acaggttcaa taggagctgt
    25021 aattgcagta aaggaattaa tcaaactaaa aaagctgaaa gggactataa aatactatgg
    25081 atgtccagca gaagaagatt tatctggaaa agtattgatg ataaaaaaag gtttttttga
    25141 tggaattgat tgtgctttta gttggcatcc atttgatata aatactccga taagaatccc
    25201 aacacttgct aattactctg tgaaatttag atataatggg attagtgctc atgcagcaca
    25261 agctccttat aacggaagaa gtgcacttga tgcagtagaa cttatgaata taggttgtaa
    25321 ttatttgaga gaacatatat ttgataattg tagagtacac tatgtaacta ctaatggtgg
    25381 aaaaatgcca aatatagttc ctgattttgc agaagtgtgg tattacataa gaggagttaa
    25441 aatggagcat gtaagagatg tatttggtag gattgttgat atagcaaaag gagcagcatt
    25501 aatgactggt actattatgg aatatgacat tataagtgga gtatatgact atattccaaa
    25561 tacaatatta acagatattc tatcacaaaa tatgaaatta gtaggtgtgc aaaactataa
    25621 tgaagatgac tactattttg cagataaatt agctgagaca gtgtctatag ataaaagaag
    25681 tagtgtttct agtgtattaa gtggaaataa ggatattact aagatgaatt tacatgatga
    25741 ggttacagat gatacattta ctcataataa ctgtatatca ttatcattgg atattggaga
    25801 tgttagctac ataattccta cagcacaatg ttcttgttca gtttggccta ttggtatttc
    25861 tgcccataca tggcaatctt gtgccagtgc aggttctgat atgggattta aggcaatgtt
    25921 attggcatct aaatctatat catgttcaat aaatgatgta atgcttgatg aattggttat
    25981 aaataaagca aaaaaagaat tgaaggatac tgtaggtagt tttgaatata ctccaattat
    26041 ttgaagagta atttaataca agtaagattg aattgtatga attaggatga atatataaat
    26101 taatattatt tttaacttaa ctttctaatt attttcaatc taagatacaa taattagaat
    26161 atatttttaa agtttcctat ttaaggaaaa taatttgatt ttatgtttga taaaaggact
    26221 tataatttgt aagccctttt attatattta ttttttgttt ataattaatt tt
//
LOCUS       DACTGW010000033        25990 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.033, whole genome shotgun sequence.
ACCESSION   DACTGW010000033 DACTGW010000000
VERSION     DACTGW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.033"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(62..490)
                     /locus_tag="I9573_001081"
     CDS             complement(62..490)
                     /locus_tag="I9573_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAT4795709.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(507..1313)
                     /locus_tag="I9573_001082"
     CDS             complement(507..1313)
                     /locus_tag="I9573_001082"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAT4795710.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(1515..2000)
                     /locus_tag="I9573_001083"
     CDS             complement(1515..2000)
                     /locus_tag="I9573_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HAT4795711.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(2051..2710)
                     /gene="spoIIR"
                     /locus_tag="I9573_001084"
     CDS             complement(2051..2710)
                     /gene="spoIIR"
                     /locus_tag="I9573_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HAT4795712.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(2855..3571)
                     /locus_tag="I9573_001085"
     CDS             complement(2855..3571)
                     /locus_tag="I9573_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4795713.1"
                     /translation="MLLIAVKKGLKFVENLTKLNLDNYKPLRDVVFENLREAILEGKL
                     KPGQRLMEVQLAEQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLE
                     VRASLEGLAAYLAAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKA
                     TNNKKLIQLINSLWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYAT
                     EHIEKAEQFMIDKAMISKDL"
     gene            complement(3519..4409)
                     /locus_tag="I9573_001086"
     CDS             complement(3519..4409)
                     /locus_tag="I9573_001086"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAT4795714.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(4527..6077)
                     /locus_tag="I9573_001087"
     CDS             complement(4527..6077)
                     /locus_tag="I9573_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HAT4795715.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(6171..6437)
                     /locus_tag="I9573_001088"
     CDS             complement(6171..6437)
                     /locus_tag="I9573_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795716.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(6585..7445)
                     /gene="yabG"
                     /locus_tag="I9573_001089"
     CDS             complement(6585..7445)
                     /gene="yabG"
                     /locus_tag="I9573_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HAT4795717.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(7529..8467)
                     /locus_tag="I9573_001090"
     CDS             complement(7529..8467)
                     /locus_tag="I9573_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HAT4795718.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(8748..9245)
                     /locus_tag="I9573_001091"
     CDS             complement(8748..9245)
                     /locus_tag="I9573_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HAT4795719.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(9360..10070)
                     /locus_tag="I9573_001092"
     CDS             complement(9360..10070)
                     /locus_tag="I9573_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795720.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(10246..10776)
                     /locus_tag="I9573_001093"
     CDS             complement(10246..10776)
                     /locus_tag="I9573_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795721.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSNKDERRVKAKANKMAMRLIHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(10793..11023)
                     /locus_tag="I9573_001094"
     CDS             complement(10793..11023)
                     /locus_tag="I9573_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795722.1"
                     /translation="MSKLVSKVKKDLEIKWLGVDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(11038..12432)
                     /locus_tag="I9573_001095"
     CDS             complement(11038..12432)
                     /locus_tag="I9573_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HAT4795723.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTI
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(12558..13811)
                     /locus_tag="I9573_001096"
     CDS             complement(12558..13811)
                     /locus_tag="I9573_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4795724.1"
                     /translation="MENKSNFKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALSLS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGVLGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(13862..15016)
                     /locus_tag="I9573_001097"
     CDS             complement(13862..15016)
                     /locus_tag="I9573_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAT4795725.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRKLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
     gene            complement(15575..16144)
                     /locus_tag="I9573_001098"
     CDS             complement(15575..16144)
                     /locus_tag="I9573_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAT4795726.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            16347..16574
                     /locus_tag="I9573_001099"
     CDS             16347..16574
                     /locus_tag="I9573_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAT4795727.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(16793..17536)
                     /locus_tag="I9573_001100"
     CDS             complement(16793..17536)
                     /locus_tag="I9573_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAT4795728.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYITDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            17704..18576
                     /locus_tag="I9573_001101"
     CDS             17704..18576
                     /locus_tag="I9573_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT4795729.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            18576..19241
                     /locus_tag="I9573_001102"
     CDS             18576..19241
                     /locus_tag="I9573_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAT4795730.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            19262..20119
                     /locus_tag="I9573_001103"
     CDS             19262..20119
                     /locus_tag="I9573_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT4795731.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGSYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(20275..22821)
                     /locus_tag="I9573_001104"
     CDS             complement(20275..22821)
                     /locus_tag="I9573_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAT4795732.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(22821..23507)
                     /locus_tag="I9573_001105"
     CDS             complement(22821..23507)
                     /locus_tag="I9573_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4795733.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(23590..24579)
                     /locus_tag="I9573_001106"
     CDS             complement(23590..24579)
                     /locus_tag="I9573_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4795734.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIVCEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(24566..25252)
                     /locus_tag="I9573_001107"
     CDS             complement(24566..25252)
                     /locus_tag="I9573_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4795735.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(25752..>25990)
                     /locus_tag="I9573_001108"
     CDS             complement(25752..>25990)
                     /locus_tag="I9573_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795736.1"
                     /translation="IEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         8568 a         4634 c         3043 g         9745 t
ORIGIN      
        1 attaaataaa aggtagtttt ttcactacct ttataaaatt tctggaaaat agccatagtt
       61 attatttagt acctatgtat atatctattt tattcctacc taaaaataaa tcttgtacct
      121 ttatattata gtctattttc aattttatgt gttccccttc ttgtatattt atatctaatt
      181 ctcttgtatc aatatcaatt aataaagtac cttgtggggt cacatacttt gtagtactac
      241 tttctccaca tttaaacagc attgttgagt taatagcacc aaactttttg atactaactt
      301 gatttttaag tatctttata gtattagtta tttctatatt ttcttctttt tccttgtaaa
      361 taacatatat tccatcattt ttataaaaaa ttttaccaaa ggcagtcatc tctatggtgt
      421 ccatatttcc tttttcatca tattgtctag tatttatact tatttttaca ctcaacgatt
      481 tttcctccat agattcttca tataaactag tatttttgta tttctacttt ttgaatctta
      541 tcaatttttt tctttaagaa ctctcttgct atatctgaaa atttttcagg tatatctgat
      601 acataatatt gataagtccc atgaatttct gtattgttta taatattttg cacctctaaa
      661 atctttttta aatcctttgc agtctcttta gctggattaa caagttttat atgctctcca
      721 acttcttcac ctattgttct ctttaaaata ggataatgag tacatcctaa cactaaagaa
      781 tctatatttt tttctttcaa ttcttgtaaa tatatctttg ctgtcaattt agccacttct
      841 gtatttgccc atccttcttc aacaataggt acaaataaag gacatgcttt attaacaatt
      901 tctatactct catctatttt agatatttcc aagttgtatg cttttgaact tatagttcct
      961 tcagttccta ttattcctac aattttgttt tttgtagaac ttacagctgt tctagctcct
     1021 gcttctataa ctccaattat cggtatatca tatttttctt gagcttcttt taagcttctt
     1081 gcagtagcag tgttacatgc tataactata gcttttacac cttttgtttt taaaaagttt
     1141 atagcttgaa aagtatattt aattatagtt tctttggatc ttgaaccata tggtattctg
     1201 gcagtatctc caaagtatat tatatcctca tttggtaata tcttcattat ctcttttaga
     1261 actgttaatc cacctaatcc cgaatcaaaa actcctattg gtttattgtt catctttata
     1321 caacacctac tttacatttt tgtgtttaac aacaacttat ttatcgctaa aatttatatt
     1381 tatattcata attaatatat tattacctaa atgattatta tagtttattt tacacaaaaa
     1441 ataaaggagc tatcttaaat tagaaaataa tttctacaaa aagacaactc ctttcactac
     1501 ttaaaaattt aaatttattt tgcaaacaca tgatttccta ttctcatata aggattcaat
     1561 ctatttatcc atttgcaagt ggctatttct ggattccaaa aatatactgc tttattagtt
     1621 gggtcagttc cataaagagc ttcctgagct gctttacgtg ctgagtctgt tggtgccatc
     1681 tttattgaac catcaagcac aactgagaaa gcattttttt gatatatgac ttcttctatt
     1741 gtgtctggaa atctagggtc caatactcta tttattacta cagctgctac agcaacctgt
     1801 ccctcatagc tttctcctct agcttctcct gcaaccaact tagataataa catcaagtca
     1861 ttatcagata aatttattac ttctttcttt gctatatttt tttcttgttt gtttactttc
     1921 ttaatatttt ctgttggttc atatgcaaat acctctttaa taggcataaa tataaaagtc
     1981 aaagctaata gtgtagccat cagtttactc cacctgtcca tgattaaaac cactccttac
     2041 attcttattt ttaaaatttt ttctctaaat ccttgactac ttcaagtact ttaaacttaa
     2101 tttttactct acttgttttt tttggtgtat cttgttttat taacttatat tctttatcag
     2161 ttaaaacttc cttcaactta tcatctgtag atttatctat cactccatta gattcatcaa
     2221 caaaacatag tggagggaac ataacacacc accagttttt tccttcacct ttcccaatga
     2281 taatctttaa tgctttatat tcaccagctg gaagaactat attagaatat tgtttagtag
     2341 ggaaattgct atattgtata cctaccttta cactgtaatt atatccattt ttaagaataa
     2401 tatttttact tatttcttcc agactactat attccttttt tataatagct tcactttctt
     2461 ttatactttt tgaattttga agctttggct gtaaatattt tattacttca tctctaactt
     2521 ttagtttaag ttcttgatct tcatctgtat tgctgtttgc tataacatga aatcttatta
     2581 atttatcttt ataatctttt gatatattgt ctactttttt tacctcacca tttattacta
     2641 ttgtcattat tgatatgact gatataagac ttaatattaa tagtcccaat ctaatcttaa
     2701 tttttctcat tgaaaattcc ccctcaagat aaatttatat catcaattat gtccaattaa
     2761 atttaattta ttcataatat tttttaatac taataaaaaa agctatctta aaatgattac
     2821 aatcatttta aaatagcttt ttaaacctta tattttataa gtctttacta atcatagcct
     2881 tatctatcat aaactgctct gctttttcta tatgctctgt agcatacttc ttggatagct
     2941 cactatcacc tttaattata gcatcaagta agtatttatg ctcttcaaca atattatgtg
     3001 atgtatcata agcagacata taggtaactc taaatctata tacttgttcc cataatgagt
     3061 ttattagttg gattaatttt ttattattag ttgctttaaa tatacattca tgaaattcta
     3121 catcaagttt taataacgca tctacatcgg attccaaagt gcttttttta aattcttctg
     3181 atatatcttt aagcttttta atatcttcat cactaattct ttcagcggca agatatgctg
     3241 ctaagccttc taggcttgct cttacttcta atacatccat aatatccttt aatgacatat
     3301 ttgccacata cgcacctttt ctaggcaaca taacgactaa accttctaat tcaagctttc
     3361 ttattgcctc tctcactgga gtcctactta ctcctaattg ttctgctagt tgcacttcca
     3421 ttagcctttg accaggcttt aattttcctt ctaagattgc ttctcttaaa ttctcaaaga
     3481 ctacatctct taatggcttg taattatcca agttcagttt agttaaattc tccacaaatt
     3541 tcaacccctt tttgactgct attaacaaca tacacctgtt tatatttttt taataattct
     3601 ttttttccaa ttagagcatc ttctttattt ttaaatagtc caaaaactgt aggaccactt
     3661 ccactcatca tagaacctaa agcattattt ttcatcatta cttgttttat atcacttatt
     3721 tctttgtgtt tacctatagt aacattttca agaatattta ccatgctttc tgatacagct
     3781 tttatatctt cagattttaa acactctatc aaatatttat tatttggtct ttttttaata
     3841 ttattcaaat ctaatccttg ataaacttct ttagtagaaa cgaataagtc tggtttgcat
     3901 atcaatatat ttgtatcaca tggtaaacct ttgatgtttg ttagcttttc tccaatacct
     3961 tgagctaagg caggtcttcc tgatatacaa aaaggaacat ctgcgcccaa attaagtcct
     4021 atttctttta attcatcttc agataatcta agttcccata ggtgatttaa acctactaat
     4081 actgcggctg cattagaact tcctccagcc atccctgctg ctacaggaat atttttttct
     4141 atgaatattt ctacaccttt ttttatataa aatttatttt taagtatttt ggcagcttta
     4201 taaacaatat tatcttcatc taaaggaata tctaaactag tgctttttac ctttatttca
     4261 tcttcgtcta attcttttat ctttactata tcgtataaat ctatcgtttg cattatcatt
     4321 tcaacaaaat gataaccgtc ttgccttttt cccaagacat caatggataa atttattttt
     4381 gctctactct ttagtcttat aaaatccatt ctatcctcct tgtttaaaga ataatgcatc
     4441 actaacaata atattatact acaaaaaata aaataatagg gtattataat accctattat
     4501 acttaattac tttaaatttt ttaatattaa tctactaata cgactttttt ttctaaaata
     4561 agacacttac caggcttaat tggttcatca agttttatat cattaagttc tgcaatttca
     4621 ttttctgttg tattgtattt ttttgctata ttccagaaag tatctccttc tttgcatata
     4681 tatacgatta tgctaggagc tttagataaa tcatatacac cttggtcttc tcctttgata
     4741 ataaaatttt ctgctttctt atctaatgct tcagtaaatc tcttaatttt gataatcaag
     4801 tctatctggt ccctattcaa atcaacttcg actttatcaa tacatgctgt attaaacaca
     4861 gatgctgtat cagttagatt atccatttct atatcatgtt caaatggaat ttcctcacta
     4921 attttataaa ccaattttaa accttcaact gggacaaata aaatttcaac ttttatgatt
     4981 ccttgtataa tgcttttatt tccttcaata taactatttt ctatagacat ggttggacaa
     5041 acgcttacaa tatcttttat ttgtatatca tcattatcat ttcttatgcc ttctctaacc
     5101 ataaatgttt cttcactacg tcttaaagtc ttatttaatt gaataggttt gtggtcaaat
     5161 tttattatct tttgaggcga atatgcatct tgtaatactt ctcttgtaac ttcatctgta
     5221 acttttacct tacaacaagc catacaatct atttctaaaa gacctgtgtt gctttcacta
     5281 ttttgcttga aaatatgatt aaaatctgac atagacaaca atacttcttc agtcatacca
     5341 tcactaactc cagggacttc tacaaattga gtaaactcaa tacctactct atccaactct
     5401 acaagttcac cctcatatgt acaagctaac ggatttattt ctaatactcc accaataata
     5461 actttattgt cagtcactct actttctttt actttaacac atggatttaa acttattata
     5521 gattgaattt cctctgtgtt tattgttata gtatctctta tagaactttc tgctttttct
     5581 attccaacta tatcttgaaa acaaatttcc tttctgtgct tttgtatgcc ttccacttgc
     5641 gccacatctt ttacaatgtc taacctttgc ttttcaaaca aacttcccct tatattcata
     5701 agagctccta ctttaatctt tctctcattc attatagtac aatccatatg ctctacttct
     5761 gaaaataaca tgtattccat atcttgaaca acattgtctt tttctattac ttcgtttata
     5821 tctacttttc cgtctacatt agaaatagta tttttatcat ctgcaatata gattacatta
     5881 taattaaaac tacctctgca aagtatcttc ccatctgcca tttcaatttt tttaagtgat
     5941 atgtatcctt ctgtttttac aatttcatat acatctaatt ttttgtctgg tacaactgcc
     6001 tccgcttcaa tgaatgtttg aaattttcca aagtctattc tattgtcaac tttaattaca
     6061 tctttaatta attccatagt ctacctcctt aagtattata aaaatatcac actatattta
     6121 tatttaagga gcaaaaaaaa aattacacct atttttaaat aggtataatt ttaacttatt
     6181 tgtaatttat cttgatctct aaaaacttgc aactttacat tagatgttaa taaatctgag
     6241 taactataag atactctagg atatccatta ctttcatcat ctaactttat aacaaacacg
     6301 cttggataaa ctttttctag tataccctct tttgtaataa tctgctttct tcctttatta
     6361 gctttcaaca atattttctt tcctatatgt ctctctaagc ttactcttat cttatctaga
     6421 gtttgaacag tagccacaat atcaccttct ttaacgttta tatgtttaca taatatcaca
     6481 tataaacatt tttgtcaaat gtttaaacta attattttat atattttttg caaaaatgtc
     6541 aatactattt ttgaagatat acagtataat tatgttaatt tttgctaatg taatattgtt
     6601 tttggcattg cccatctaaa ttttcctctt gtttcagaac caccaattcc cttaacacct
     6661 gtaatagtct gctctataac ctcatcaata ggaactacta catcaattct tgaacaagca
     6721 attttctcca ctataaaaac agggtctaat gcatgtatca ttattcttcc aggcgaactt
     6781 gcataatttg ctcctgcact tattattttt tcataatttg attggcatgc tcctgcgaat
     6841 atgacaagat tatccaaatt tggttgccat ttacgtgctt cttttactgt tttaacaaaa
     6901 tttgaagagt ttctatagtt actcatatct tctatatctc ctctttttat tgtcattgca
     6961 tcatgacctg ttattactag tatatccgga ttatattttt ctagtagtgc tcttacttct
     7021 ttatactgat tttgctctga aacagctaca cctactgcag gtattccaag ttgagcatat
     7081 acatccaagc aaatttttaa atattcttta tctccatcta tctgtagtac ttttccaggc
     7141 attccatatg tgttaggatt taattgaagt tttggaactg ctctagtcat tcttttttgt
     7201 ctttctttag cttttcttac tgacttatat aataaatttt ctacattttt atctattaat
     7261 atatctttta tatcaggtgc ttttactaat tctaggtcat caatataagc atctgctatt
     7321 actctaaatg caattccctt tagtatagct attttctcat tattttcatc tacaccaaaa
     7381 gagactattt taaatactat atctttgtta tgtgactttc tagctacaat gtctcctacc
     7441 ttcatagtta aacctccaat aaatttttat cttagtttat actatgttcg atataaaact
     7501 ttgtttccta atgtcaaaaa aacatttctt ataatacttc ttttatcact cttaaagcat
     7561 ttttatagta tatcttttct atatcatctt cactaaaccc ttcttttttt agaggttcat
     7621 acagtttacc catctgagat atatcttcaa tctcaacttt tgaatctatt ccatcaaaat
     7681 ctgaccctag ggatacaaca tctattcctc caacatttac aatatgtttt atgtgtctaa
     7741 ccatatcttc aagtttactt tcacttttat cacttaaaaa taagtggaag aagtttatac
     7801 ctgtaactcc acctttattt gctaatactt taatcatatc atcagttaaa tttctagaat
     7861 gattcatcat tgctcttgaa ttagaatgtg tagcaattat tggtttagaa gaaatctttg
     7921 caatttcata aaatccacca tctgaaatat gagaaacatc aaccaacatt cccaattcat
     7981 tcatcttatg taccacttct cttccaaaat cagtaagccc tttttctcta tattctactt
     8041 tattatgagg aaagctcaat tcattcacat gattccaact tattgtcatc attctaaccc
     8101 ctaagtcgta aaatttcttt aaattttcta atttaccttc aagtactgca ccttcttcaa
     8161 tagataaaag agctgtaagt ttaccttctg attggttttt cataatttct tcatagttag
     8221 tagctaaagc tatttcatca ctattttttt tcatttcctc atgaaattta tttgccatac
     8281 tcatgcaata atcaaaagga tgttttactt cttcagtatc aacaaaaaga gcaaatgttt
     8341 gagctaatga atcccccttt ttcaatctgt ctatatcaac agaatattta ttagacttaa
     8401 gttcacttgt ttctacattt tccattaatt ttgctatagt atcacaatgt aaatcaataa
     8461 atttcatatt tataccccct gtatatttta tatttatatc taaaaataga aatagttgtc
     8521 cttatagaca actattgaca actatttttt aatattcttt ttaatattac ttaatcttat
     8581 tcttctttta agttaggtat tactttttta acttcttcaa tttgatgcca tgttttatct
     8641 atattatttt cactaatata tttttttaaa tcagttaaag atgtaaaatc tttaactgat
     8701 aaattgtcat catcatctgc aaatcttaaa ataaacatga gactccctta ctttccacaa
     8761 catttcttat atttttttcc gcttccacat gggcatgaat catttcttcc tatttttgct
     8821 tctttaacta ttgttttaga cttcttataa gcctttatta tttcttttct ttggtctgct
     8881 ggtagtatat cttcccaacc ttgtaaatta tatagccaat gagcctcaac agttaccata
     8941 ttgttatata atttttccca atctattttt atagaaactt ctttatcagc ttctacttct
     9001 tctaaaacta tatcttcttt taagctttca cttattccat ctatgaatcc catgaaaaat
     9061 tcattagtag ttccaaattt ttcagccaat tcacttactt taccttcaac aacatccatt
     9121 ttattattta atatttcatt gtatattcca gcttcaactt tcaaatattc ttcccagaat
     9181 tttacttctg cttcttgact ttcatgatta tcacttaaat ttctccattc agtatataaa
     9241 ctcatacttt ttcctcctaa gtatacctat atttttatag tttttatagt ataaaatctt
     9301 ttagttagat tggaagtttc ttctttttga ttataccata ttctatttaa agttaactct
     9361 tatattatat cataattaaa caattctttt aatactttta tagcatactc ttggtttata
     9421 aaataagggc ttgtcgcaat tgttattact ttggcttttt taattaagtt ttttatcttg
     9481 taaaatttga gttcaaatgg tatatagtcc atatcacttg aaaatatatc caaatctata
     9541 tcaagaacaa actctgattc tacttctaaa tcaaatccat acagactatc tattattatt
     9601 aattctgaaa atatgttata ttttaaagca ggttttataa aacttcccac atttaaaact
     9661 tcatttgtat acctaaatac atcttttaga ttatttacat ctacatcata gttctcaggc
     9721 tctctagtat ccttatgttg gtctacatgc actaacttgc atccttttgt aaattcaccc
     9781 tttaacaaag attttgccca aaaataaaat gcatggttat gattgtcaaa cacatatatc
     9841 tttttatttc ccaggacata ttcgaccata ttctccaatc cttttgcttt tatttcctta
     9901 ttttcatcta cttcattaaa taccacctca ttgcctaacc ttacatcatt taatgtacca
     9961 tttataagtt ttggaacgta tatgctctta ttttctcttt tatcatatga aaatacatta
    10021 tttcctactg gtttttctat ataaaatcca ctatattcat aattttccat aaaactttat
    10081 ttcctccatt gttatataaa tatataagtt ataattttaa aaattaaaat gcacttattg
    10141 taaatcatat tatctattat agaaaacatc ttatataact aaatataata tcttctctca
    10201 atatgtatta tacaataagt gcaaaatata tttatataaa atatactaca ttcctataac
    10261 aaaaccatca gcttcaattg gaaggtaact aactcttcta ttcaactcca cctttccaaa
    10321 aaatactacc caatatggtc taaaaatact ttcaatttta actatttcaa tctctgggat
    10381 tcctcccata aacctatgaa ttaatctcat agccatttta tttgcctttg cttttactct
    10441 tcgttcgtcc ttattactat actgcacaat attacaatcc acatttttta caatttcata
    10501 tgtaggaaaa tcttgtacta tactaccaga acctgtagaa ccattcacta aaatttttaa
    10561 ttcatttata tcattagaat atctcctatt cttgaaaatg gatttcgttt tgtgaatttt
    10621 aagtgtgata attttaaact ctatataatg aagtctaact tcatgagcat atttgttctt
    10681 taatattagc ttactaaata aagaattttt cccactttta gctatctcta tagcttcttc
    10741 ttttgtaatt cttataggta acgctcttat atccataaaa tcattctctt tcttattttt
    10801 ttcttgtcat ttttaaaact ctatatatat aatcaaaaaa tattacaatt acaactgcca
    10861 taactccata tggaattatt tttccaacta taatatcatt tctagtcaat ataccataaa
    10921 atataaatcc tgttattaaa aagtacaata atactagaaa accgtctact ccaagccatt
    10981 ttatttctaa atcttttttt accttagaca ctaatttaga catagcagtt tctcctttta
    11041 tagattattt tgtgtattta aattttcacc tattctatta ccaatcatcc atgcaataaa
    11101 tgaaaaaggt atagctatca caagaggctc taactcaaag ctctgaactg gaaatatttt
    11161 aaaataacca aaagtcactc cacctacaat cattcctaaa ataccaccca tagatgttct
    11221 tcttccattt ccaagaagcc cacctactat aggtacaaat gccgaaccca tccaaatact
    11281 tcctacaaat atacatgcat catatacatt ttcaaatcta aatgctacgc taagccccat
    11341 aactgctgct aaacatacac ctattctagt aaactttact aattgtttct gagataactc
    11401 tttttttgta attcttccat atatatcatt tgctaatgta gctccagctg ctagataata
    11461 gctatccaat gttgatatta tagaacctaa aattcctatt ataaatatta ctcttattcc
    11521 ttgtggtaat gtatctaaaa ctaaagttat atatccagcg gctggagcca tctctggata
    11581 agtagctctt actataagtc ctgttatagt taacaccatg tcaaaacata tccatagtga
    11641 aaaacaagtt aataaggctc ttcttccaac cttaggagaa tctgctgctg aaaatctttg
    11701 ataaaaagtt gggtctgaat aagctccaaa agaatataca attaacatca atgctttcat
    11761 agctgacata ccttttgtag ggtgcataag ctgtggtgca tctttgagtg cttctgtgag
    11821 accttgaaat cctcctattt gttcaaattg acctggcata accatagtta aaccaaatac
    11881 catacaaaaa aacaatacca tatctgtaat ggcaactccc ataagcccac caagtactgt
    11941 aagtacaacg actactccta ccattattaa tcctattaat tctttatcta ttttccatgc
    12001 tacgttacct atagttccca tagcagttat ttctccaagt tgtgcacaat atataaacat
    12061 caaaattgcc ccaattatag caacatgctt accataaaaa ctttccagta aatcaggaac
    12121 tgttatattg gttcttatat gaatccttgg accaacccac aatgctaaag gcattctccc
    12181 aatatgtgca gctattgacc aaactgccca tgcagatatt ccatatatag ccgcatactc
    12241 tacactacct actgtaccaa ctcctccata ccatgatgca accaaagttc ctactattaa
    12301 ggaccatggt aaacttcgat ttcctaggta ataactttcc atatctttta catttctaga
    12361 aaaataataa cctattccta ataatataaa tgcaaatata actataatcc cattttccca
    12421 aaatgttaac atatctaatc tccacattct tatattttaa tttttaactt taatatctaa
    12481 atatgaacaa ctctacccat ttccataaaa ataggtagag ttatataata attataagta
    12541 tttaagtaat cttgttatta agcaatttta ttgtttaaac ctttattttc tcttttattt
    12601 attttattta aagcataagt agcaaatatc cctataactg cacatactac agataaaata
    12661 aatatatatg tatatcctgt ttgtccatat ttatctaacc aattacctgc aatagtgtaa
    12721 taaaacgcat ctggagaaaa tcctataaat gaagcaaatc caactactgc tcctgtagtt
    12781 tctaaagata tattagactc tgaaacagat gcaaagtata tacctctaaa tccaaataaa
    12841 attacactta gtattatcat gtttattaca gctatccata taaatcctgt actcttagga
    12901 ataaccaagt aagcagctgt acttactgtc attaatataa atcctacaaa cattactctt
    12961 atagatgaac caactttatc tgttataaca ccagcaactg gagatgctcc catttttata
    13021 acataagttc ttattattga taaagcagaa actagagtca ttggcattac ataaacttct
    13081 gataagtatg gacttaaata agttaaactt gagaatacta aataagttga aaatattata
    13141 agaccaatta accaagcctt tggcattgta actactttta acaatgactt aatacttaat
    13201 ccttcatctg atgctccact atcttttttc tcttccacta agaaaaatag aagtatacca
    13261 catattatag atgctgcaga atataaccat actacatatt tgaatcctac tgttatttca
    13321 gcaaatttac caaagaagta aagacctata aaagaaatta acgtacctga tacacctgat
    13381 aacccttcat aaaaaccaaa tattcttcct tgttctgact cacttccttg cattctaaca
    13441 accttaacag atgctgagaa gaaagttaat attgttgtaa atccccataa tacaaatatt
    13501 aagagtatca tactatatga tgggaatgta gagaaataaa aaccaagtac tcctgaagca
    13561 attaaagaaa aagttatcag ctttctaacc gagaatctat ctgcaaacca tcctcctata
    13621 aaataagata ccattcctac taatccatat aaactcaata agttccctat ttgtgtatgg
    13681 ctcaatgata aagcttgttg cattgggtca taaaatgatg atttcatata tggtaaacta
    13741 tacatagccg ttgttccaaa tgccaatatg aatattatca agaatctttt aaaattactt
    13801 ttgttctcca ttaattttct ctccttaaaa ttttattgtt tataatctca ttcatgtaat
    13861 tttattttaa tttttttatt gcttgtttaa agtcatttat tatatcattt gaagattcta
    13921 atcctacagc tattcttatt agattttctc cattcatttg aactaatgta tgctcttccc
    13981 ctaagcttgt tgctattgtt gcaagctcta aagagttcat taattttcta ctagcttggt
    14041 ctcttgtcaa tccattgata ccatccttaa gtttaaatga aactacacct ccacctaaac
    14101 cattcatttg agttttacaa agctcatatt gaggatgaga ttttaatgct ggatacttta
    14161 ccaattccac attctcttct tcctctaaaa actctgctac tgctaatcca ttttcacaat
    14221 gctttttcat cctcatatct aacgttttca tacctcttaa aatcatccat gcactcattg
    14281 gtggtaaaga tgctcctgta aaacaacata aacttggcca tctaactaaa tctattaatt
    14341 cttttgaacc tgctactgct ccccctaaag tatccccatg tccatttata aacttagtaa
    14401 gactatgtat tactaaatct gcacctaaat ctattggtct ttgtacaatt ggtgtcccaa
    14461 aagtactatc aactatagct aagcttttat tttcatgagc aatttctgct attgttttta
    14521 tatctactaa gtctaatgat ggatttaaag gactttctat ataaactatt ttagtatttg
    14581 gtctcattgc ttcttttaca ttttgtggat ttactgcatc tacaaatgta acttcaactc
    14641 caaatttaga taacaactca gacatgaata atttagtgtg tgaaaatatt ccatctgaac
    14701 taactatatg gtcaccactt tttaccaatg ctaatatagt agatgttatt gcccccatcc
    14761 ctgatgtact agatatggca ctttctgcat tttcaagcat agccaactta gcttctaact
    14821 ctgtaagagt aggattacac tctcttgtat acacatatcc ccatgtcata cacaactctt
    14881 caaaatgctc tactgtcttt gcagcaaatg ttgctgtttg acatattggt gtagccaatg
    14941 cacctgttgt tgggtctgca ctatgtcccc aatgtattat ttttgtctct aaatcttgat
    15001 taatattgtt actcattttg atttcctccc attttgactt atttatacaa cgttttccct
    15061 gccaaattta atcaatttag cttagtctta atacattttt tcaagtatac ttttttattt
    15121 gttataattt ctaaaaatat gacacttatt ttgttttttt acagttctaa ttataaactc
    15181 tatagtaact atagagtcaa tatgtttttg atattttttt tacaatgttt aaaatagtca
    15241 gtacaaaaat aggtatatct tagaataact aaagtcatcc taagatatac ctatttttat
    15301 atcacaatat aaataattac atataagcat tagttttttt atcataaata gatttttcaa
    15361 gatttatcat cttttcataa atatattata gttaaatatt ttaatattac ctaacatcta
    15421 caataaaata tactcttaaa ataaaattat ataattaaaa atactctata aaagctaaaa
    15481 agttttctat tcgctacatc taaaaataat tagatagagg tataaatgta tttagtttat
    15541 ttatacctct agtcttttat aaattatatc tctactaaat tgatttttta gcacgaataa
    15601 gtgtgctctt ggagatacca gtcatcttca caacctctct atatggctta gaatttaata
    15661 accccaatgc atattgtatc tgttgattgc tgtatttctt tggcctacca tctttaaagt
    15721 cctttttctt tttagcaaat tcttttccta attgagtacg ttctattatc atatttctct
    15781 caagctctgc aactgcaagt aatgtagtca aaaagaattt acccattgaa gtattttcta
    15841 aaagacctat atttaaaata tgaacactta cttctttagc aaataaacct tctattagtt
    15901 gtatcccttc tgatgtactt ctagctagtc tatccagctt agttgcaaca agtaagtctc
    15961 ccttttttag agatgatata attttttcaa atttaggcct gtttgtagta gtgcctgtat
    16021 aagtttcctt tattatttta gcatcattat atttattcaa aatttcttgc tgttgctgat
    16081 acaaactgtt gttgtctgtt tgattttttg tacttacacg gcaatatcca tatataataa
    16141 tcatcacttc ctaacataaa gattttgtac agaagttacc cagaagcttg tttttactta
    16201 tctttatcca ttttactaaa acgatttttg ggtgtcaata ctgttaactt ttgacaccat
    16261 aataattcct ttataatttg tttgactatg tcaaaaatta ccaatataat gaaattaatg
    16321 agtttataca aattggagga aataatatga atagtctgtt aaaagaagca atagatgaat
    16381 taaaaaacat ccattgcggg gaagtttttt tattaaaaga attgttcaaa ggctatgaat
    16441 ggaacaggtt ggagatgaat attagattga aattaggaaa actttttttg tatgaagtgg
    16501 taactaatcc caatttaaat gtagaggtca tcaataaaaa ttctgcttat cagcaaatgt
    16561 ataaaaaaaa atagtgactt aaatgtatat aatagtataa tcttcttatt gattcaaaag
    16621 ttaaaacttt taatacaaaa caatcattct attttaacat ttatcaatat gtctaaattt
    16681 atataattaa cacatacgaa tagtattgtt taaatcacat tatttaagat agataaatct
    16741 taaccctact ccagcataaa tactaaaata gggcttagaa ttatctatat ttttatccca
    16801 taaaatgctt agctcttcct cttgattgaa catatatatc tggggaaata caatagaagc
    16861 gtaaatttat aaaatttcca aagtcagaaa acttcagttc aaacttaggt ataccaaaac
    16921 tttcaggtgc tatttcatca acgccaaaat agatgataat tccattatct gtaatataaa
    16981 atgcttggtc ttcaggtata actaactcaa cattagaata atacatttct ctattttcat
    17041 ttatttgaga tttaatgtaa tcagtaatta tattaatata atcaactcca tctctaaaaa
    17101 tgtcattaag taaaagttct tttccagttg tcaaatctat attgtaatta ttcaatgtat
    17161 tgtatttagg cccatagtta ttagcaaacc ccataaggtt taatatcata ctcagtacat
    17221 aatttttatt aaaagtaact gcgtaactgg tagataccca atagctttgt tttggaagtg
    17281 aattttgaga agctgcgtta ttatattctc gttcttcttc ctgtaagcca tttttaaaat
    17341 tatttacagt cattcttaca gcattgttta tgttatttaa aataactggg tttacatacg
    17401 tggattgatt tctaatattg tatataacag gatactttac atcatatgaa aaatacttac
    17461 ttctttcaaa atcttcatta aattgtacat ataatatatt atatgtacag gtatttgtag
    17521 attgataatt ttccattaaa atcctccccc ttttataaaa aaatcagata caatataata
    17581 tgcttaaaac agtaataatg ttaatagtaa atgtaaagaa tagaaagtat ctaaaacagc
    17641 aaaaaatgac taattttgtg gtataataca taggattgag agttatttta taggaggaaa
    17701 aatatgttat tattagcaga taaatggaaa gattatgaac ttatagatat gggaaatgga
    17761 gaaaaattag aacgttgggg aagttatgta cttagaagac cagacccaca ggttgtatgg
    17821 ccaatggaat ctgaatgggc tttatggaaa aaccctcatg gacattatca cagaagcaat
    17881 aaaggtggag gtcaatggga acacaaaaaa agatatccag aacaatggac tataaattat
    17941 aaaaacttaa agtttcacat taaaccaact ggctttaaac atactggatt attcccagag
    18001 caagcagcta actgggattg gatgatagag aaaataaaga aagctaacag acctataaaa
    18061 gtgttgaatc tttttgcata tacaggtgga gctactgtag catgtgcatc tgctggtgca
    18121 gaagtatgtc atgttgatgc tgcaaaaggt atggttaatt gggcaaaaga aaatatagaa
    18181 ttatcaggtc ttaaaaatca aacagtaaga tttatagtgg atgatgttgt aaagtttgtt
    18241 gaaagagaaa taagaagagg taaaaaatat gatgcaataa taatggaccc tccttcttat
    18301 ggaagaggtc ctaaaggaga agtatggcaa atagaagaaa agttatacag ttttgtagat
    18361 ttatgtatgg gagttttatc agataaccct ttatttttct taattaattc ttatacaaca
    18421 ggattttctc caatagttct tgaaaatgta ttgtctacaa caataggtaa aaagacaaat
    18481 acaggagata tatacggtgg ggaattagga ataccagctt ccagagatgg taagatactt
    18541 ccttgtggta tatttggaag atgggagaaa aaataatgat aaaagtcata tatgaagata
    18601 accatttatt agttgtagaa aaaccagtta acatattgtc tcaaggagat aatactaatg
    18661 ataaagatat ggtaaatctt ttaaaagact atattaaaaa aaagtataac aaacctggaa
    18721 atgtatatat tggtttaata catagattgg atagaccagt aggtggagtt atggtgtttg
    18781 caaaaacatc aaaagcagca tctagattgt ctgagcaggt aagaagtaag actttcaaaa
    18841 aaacttatct agctgttgtt catggcaaga taaaatctaa ctcagatata ctaaaagatt
    18901 ttttatataa aaataaaaaa actaatatgg taactgtagt gaaaaaaagc cataaagatg
    18961 ccaaagaagc tgaacttaac tataatgtat tagattttaa agaaaatcta agtttagttc
    19021 aagttaattt aaagactggt cgttctcatc aaataagagt tcaatttgca agcagaaaac
    19081 atccattata cggagaccaa agatatggca aagaaataaa taagattgga caacaaatat
    19141 ctctttggtc caataagata gaaatttgcc atcctactac aaaagaaaaa atggaattta
    19201 catgtcatcc acctgacaaa tatccatgga atatatttta aatctcaaat gaggtgatta
    19261 tatgagtaaa aaattttcaa taggagaaat gtctaaactt cataaaataa gcatacagac
    19321 actaaggtac tatgaccaaa tagatttatt taaacctgtt catgttgacc aagaaagcaa
    19381 ttacagatat tactcaattg accaattttc acaattagat attataaaat atttaaaata
    19441 tttaggtatg cctttaaaag aaattaagtc aaagcttgat gaaaataatg aagaaattct
    19501 aaaattacta acaaataaaa ttaatatagt ggatgaaaaa attaaagaat tagagcttat
    19561 aaagaaggta ctaagcacaa agaaagaaac tatggaaaaa ggtatgaaag tagatgaagt
    19621 tggtaaaata gtgagaaaac atattccacg aagaagtatc ttatctatag agtatgaaga
    19681 aggattaagt agtgaagaaa ctatggaact ttccagaaga caaatagcaa atatattaga
    19741 agataatata tctgtgttct atggtggagt aagtggactt gtatctatag ataaattttt
    19801 aaaagaagat aaagttattt atgacaatag ttttgttact gttgaaagag acttatttga
    19861 tataaaatca caaaaacata taacagaaat ttcatctggt gattttattt gtatgacata
    19921 taaagggtct tacaaagaaa attataccgc ttttaagaaa ctggttgatt atgttgaata
    19981 taaaagaata cctattgaaa attatatata tgaaatacct ataatagacc ctctgtctac
    20041 aaacaacgaa gaggaacttc taacagaaat tcaaatacaa gttaaaaaga gagaaattga
    20101 cttaagatgt ctagtgtagt aatctagttt acttacacat cttaatatag ttgtctatta
    20161 tcaacatatg atttttctag atagtgattc ctaacagtga gattttcaaa aaataggagg
    20221 aaatatggct aatgctaatt ccctcctatt tttaattaat ccatatcatt ttaattaagc
    20281 catgtcatca cgaagtgcat caataatatt ttcttttttt atcttattaa tagtatacaa
    20341 cattgtaata aatactataa ataaaacact aaatatacta attccaatac taaaccatgg
    20401 aatcacaaaa tcaatatcat ctgtaaccat tactttatga attagccatg aagaaattac
    20461 tgctatagga agtccaaaga acaatgctct catgccataa aaagcacact caaaattcat
    20521 catcttttgg aaatcatgct cagacattcc tacagagcga agcatggcaa gttctctacg
    20581 acgtaactta atattagttg aaattgtatt aaacacattg gcaatcgaaa tcagagaaat
    20641 cataattatg aaagtatagg caaaaacatt ggcaataaaa atgtaattac ggttctcatc
    20701 catcattcca tacatattgt acagattgta ctcattagta actccctcac tctcaataat
    20761 ttgctccatt tctgcaactg actgagtagg atttttcgat tgaaagctta aactttcaat
    20821 tcctgtagaa acaccaggaa tctcaaactt atctttggct gaataaggag ccaatactct
    20881 gaagcttgct ttcttcacat ctgaacttct gattcctgga ggagaatcag gtggcactac
    20941 tttgacaaat gaaatattca cattttgtct ctcaggtatc tccaacttgc cattagtttc
    21001 aggagtgaga ctaacattca tagaagaact gttgaaaata tcaataatat tattatcctt
    21061 tgttaccata gaaacagcaa tcagttttgc attttgtcca gtatattctt ctgctggtaa
    21121 tccaagactt ttaataatat tcagataagt tctatcatca agaaattgca tatctactgg
    21181 taattcaagc atttcactca tagaatgtga accctcatag ttcaaataat cgtctgtaag
    21241 ctcacttact ttggcaacac atgaataggt cataagtttc tgatacaaac cttcgtaaac
    21301 accatttgca gtttttagct tatcgtaaag cttcaacatt tcgttgtctt tcataccttg
    21361 tgtagaaaaa ataatatcct gatcagtaac ctctgacctc tgatttgaca tctgttttag
    21421 gtctgctact aaagcacttg tagatataaa taaaactatg cttaaaacaa gcgacaatac
    21481 aatacttcga taacgtttct tgtttctttt aaagtttttc atagcaagaa ctccctcaag
    21541 accataaact cgctgtgtta gttttgatgt cttcacagat ttagattcaa tttttacctc
    21601 atttgtctga cgaatacact ccatcactgg tgtgttggca gcctttcttg ctggtatgta
    21661 agcagaaatt aagattgtaa ccatacttac cactgctgca acgatgattg cagggataga
    21721 caatgttaat gtcaacggaa cgtttacata gagaatattt gcaaagtttt tagctatgac
    21781 agaaatcaca agtccaatac tgactatacc gattgcaaca ccaattggta taccaatcgc
    21841 accaatgcaa attccttcaa atagcaccga atttcgtaac tgttttgctg tagctccaac
    21901 agatgaaagt attccaaatt gatgtgttcg ctcgttcaat gacatgttaa atgaattata
    21961 tattagaaaa attgagccta gcatgattat ggcaagcaca atgccaccaa ccgagtacaa
    22021 aaacatattg aacaaattgt tatcagaaac acctatgaag cgcaacacat tgtcattgaa
    22081 aatggaagca tggtctttag tgctacttgt gtaagagcga atctgatgtg gatttttcaa
    22141 tgtgacaaaa aggctaagac tgtcagtctt gacttgttta tcttctttgg ttatcaaggt
    22201 atatcctggt gcagaagatt cttcaaatac gggctttcta cagataccca caactgtata
    22261 agttttctta tcctgtggca caaaaacttc actcccagca gtgtaggggt cactctgacc
    22321 aagcttttca ttttcactca ttcgtttccc tacatccaaa gaaatagtat cacctactgc
    22381 aaatttgaca ccaccatctg tcgcaacttt acttgaaaca agaatctctc tactattttc
    22441 aggtaatctt ccagaaatta aagttatagg caaagtatca aaagcttctt tgctaaatcc
    22501 agcaataaaa agatatggtt tatctggatt tctgttacct tcaagtgttg catagccaat
    22561 attttcaaat gttgcagtat ttgcaacttc atcattgact ctttgttcct gtacaaaaga
    22621 agaatccaca tccaaaaact ggacatgcca atcaccatat tttataatag ctccatttgt
    22681 catatagttc atcaaagaaa ctccaaaggt agtaactgct gtaatcatag cagctgacag
    22741 aacaactcct ataattgtta caagagttcg agtgcgattc tttttcaatc cttgcagagc
    22801 aactttgtta aaaatgttca ttaacgattc accttcctct catcacgcac cacttttcca
    22861 tctgaaatac agataattct atctgcctgt aaagcaatgt tttcatcatg tgtgacaata
    22921 atgagagttt ggtgatattt actatggctc tctttcagta actggataat ctcttgtcca
    22981 tttttactgt ctaagctacc tgtaggctca tcagcaagca taactgctgg tgcattcatc
    23041 aatgcacgac caatggcaac acgttgctgt tggcccccag aaagttgatt tggtaaatgt
    23101 gtttttcgtt cttttaaacc aagtaattct aacaagtcat ttaaccgttc ttgattgacc
    23161 ttacgcttat ccataagtat aggcaatgtg atattttcca ctacatttaa tgtgggtatc
    23221 agattgtgaa actgataaat caatccaact tgtcgcctac gaaaaatagc aagtttttca
    23281 ttgctttggc catatacatc ttgcccctcc agatatatct ttccacttgt tggtacgtct
    23341 actcctgcaa tggaatgtag tagtgtagat ttaccagaac cagaagaacc aatgattgca
    23401 gtaaactccc ctttttcaat agtaagtgag acatggtcaa gtgcagtaac cttattttca
    23461 ttcttaccat aaaccttaca taaatcttca atctttaaaa acgccattat ataagtctcc
    23521 tttcatattt tataaatata tcatagaact ttcaacttac atttaagtga catttaaatg
    23581 agagattcgt cactttggaa aacgaataac aaatattgca ccaccttgtg ggtgattttg
    23641 tgcagtaatt gttcctcctt gtctcataat aatcgtctta caaagtgcca atcctattcc
    23701 ataaccagct gtgcttgaat ttttcccacg ataaaatctg ttaaatagac atggtaagtc
    23761 ttctttttta aagcctgctc cgttgtcatg aatagtaagt tgagtaaaca aaaagttgtc
    23821 ctcacaaacg atatcaattt ttccattatc acccacactc tccatgcagt ttttaaatat
    23881 attttgaatt gcttctgaaa gccaatttaa atcacccaaa atccttactc cttttggtat
    23941 atcaatatgt aaagtgatat tgtgcagttc catgggaatc aaaagttgat gaagtgcaga
    24001 actaatcaaa ttattcacat caatttgttc tttttgaaag actacaatac ctgcatctaa
    24061 acgtgataat tttaaaaggg aagtaatcag ccagtccatt cgtataagta attcctctgt
    24121 ttctcgtatc aactcttttc tctcatcttc atcagaagta ttctctaaca gtgacaaaat
    24181 aagagttact gatgtaagag gagtgcgaag ctggtgggct atgtcagcta gtgagtcagc
    24241 aaggtgttct ttttcttttt tcagtgcgta attttgctct ctaatacgta acgtcatttt
    24301 agttatttca ctttgtagaa tagaaagctc accctcatct gattcagcaa tatacagatg
    24361 gttagcatta tgaagcacaa gattaatttg ttctgaaatc tgtgcaatac ttttgtatcg
    24421 atttttagta aacacaaaaa atgctattcc aaaggtagtg gcagaaacaa tagaaagtat
    24481 tccagtcata atactgattg caaatcccag ggctacagtg agaatagtaa ttaataagaa
    24541 taaaatagca aattttcgaa actctctatt tcgaaacata acttcccccc aatctatacc
    24601 ctgtcccacg aacagtcaaa attatttgtg gacttgaagg gtcatcttct atcttctctc
    24661 gcaagcgttt gatgtacaca gtcaatgtgt tgtcattaac aaactcacct gccgcatccc
    24721 acaattcatc aagtaatctt accctcgtaa taatactttt aggattgcta ataaacacca
    24781 ataacaaacg gtattctaaa gctgaaagaa aaacctcatt gccattcttt ttcacaacac
    24841 cggttgccgt atctacataa agcccacaaa tttcaaaagt tgacccaaaa cgcccacttt
    24901 ttcgtagtgc tgttctaatt cgcgcaatca gttcacgtgg acgaaaaggt ttagtaatat
    24961 agtcatctgc acccatattc agtccagtaa caacacttga ttcatcacca gaagccgtca
    25021 aaaagataac aggaatgtct tgcttttctt tgatttctgt gcaaatcgta aacccatttc
    25081 catcaggtaa agaaatatca ataagtgcca aatcaaattt attcttagca atctctacaa
    25141 gtgcttcact tcttgtggat acatgggtta ctataaatcc ttctgaatta agcaaaagaa
    25201 taagattttt ggcaattgct ttatcatcct caactaaaaa tatccgtttc atttactttc
    25261 atcctccttt attttattag ttttatatat tccagcagag caatagtaca ctactatttt
    25321 acttatcatt acacaatttt cagtagtttc ttttgttctt atataatcca tgcataatac
    25381 ctccattgat ttattatatt tcttataaca taaatacaca agataagaca aaaaacataa
    25441 tgtaagtgca agttttttaa ctcactaact tataaacata cactcctact aacactactt
    25501 tcttgatgac acatcttaca agcttatatt catacatcaa tgaaaaatta aatttatcca
    25561 tttattaaaa taaaaggcaa tagcttctat agctaatgcc ctttatctta aaatgattta
    25621 aatactatac aatttttata aatggctata aataaactcg tatagaatat aaaatttatc
    25681 tacagtcttt tgttatacaa aatttataac ctcttataga acaaatttac cactttgtaa
    25741 acttatcact tttactcagc tatattataa acttttaatt tatctttgtt taaatgtttc
    25801 ttcttaactt ctactaaagc ttttagcgta tccaaagatg tcattatttc agtagaaaat
    25861 tcaatggctg ttcttctcat cttaaatcca tctcttgtag agtcatttcc tttagttggt
    25921 gtatttatta ctatatcaac ttgtttattt cttatagcat ctaatatgtt tggtctaggc
    25981 tcttcaattc
//
LOCUS       DACTGW010000034        25801 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.034, whole genome shotgun sequence.
ACCESSION   DACTGW010000034 DACTGW010000000
VERSION     DACTGW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.034"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            154..900
                     /locus_tag="I9573_001109"
     CDS             154..900
                     /locus_tag="I9573_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAT4795737.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            962..1636
                     /locus_tag="I9573_001110"
     CDS             962..1636
                     /locus_tag="I9573_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HAT4795738.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCLNCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCALRCMMSCPWSL
                     RYANQK"
     gene            2004..3350
                     /locus_tag="I9573_001111"
     CDS             2004..3350
                     /locus_tag="I9573_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795739.1"
                     /translation="MEIETKTPDWINVLELNSKIDITWKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIVGTTNFITRGVSNI
                     SIITLEGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLFHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     REIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITKCGQYYKSVDYNTYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNKVMLTC
                     YAEADELIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSIEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3377..4777
                     /locus_tag="I9573_001112"
     CDS             3377..4777
                     /locus_tag="I9573_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795740.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIIDAAPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GEPGHYENLFFSGFSGIALDMPNNSTANDITVFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKVIKMHKPEVGDIMSTIIKDENILSAHYGGTWGKIGE
                     KIEFGITVHYYKLIEK"
     gene            4987..6423
                     /locus_tag="I9573_001113"
     CDS             4987..6423
                     /locus_tag="I9573_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795741.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPDLQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSPTETTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKDYEGYGSYKINNITIDQCGYCGFLFDSVKGSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIK"
     gene            6767..7711
                     /locus_tag="I9573_001114"
     CDS             6767..7711
                     /locus_tag="I9573_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HAT4795742.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7880..8812
                     /locus_tag="I9573_001115"
     CDS             7880..8812
                     /locus_tag="I9573_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAT4795743.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8917..9384
                     /locus_tag="I9573_001116"
     CDS             8917..9384
                     /locus_tag="I9573_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HAT4795744.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGETMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9628..10482)
                     /locus_tag="I9573_001117"
     CDS             complement(9628..10482)
                     /locus_tag="I9573_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAT4795745.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10648..12612
                     /locus_tag="I9573_001118"
     CDS             10648..12612
                     /locus_tag="I9573_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT4795746.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLSEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSCCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFVKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLLEESI"
     gene            12612..13073
                     /locus_tag="I9573_001119"
     CDS             12612..13073
                     /locus_tag="I9573_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT4795747.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSDAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            13076..13396
                     /locus_tag="I9573_001120"
     CDS             13076..13396
                     /locus_tag="I9573_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAT4795748.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13412..14512
                     /locus_tag="I9573_001121"
     CDS             13412..14512
                     /locus_tag="I9573_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAT4795749.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14632..17232
                     /locus_tag="I9573_001122"
     CDS             14632..17232
                     /locus_tag="I9573_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAT4795750.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKHVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDYILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNVFIEESGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLILKDSGNAGDTYDFSPLKNDEDIKLSFDRVYTEKTLGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPEKY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
     gene            17701..18036
                     /locus_tag="I9573_001123"
     CDS             17701..18036
                     /locus_tag="I9573_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4795751.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            18173..19450
                     /locus_tag="I9573_001124"
     CDS             18173..19450
                     /locus_tag="I9573_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HAT4795752.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNNSQKDSNNDNNDNG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            19571..20959
                     /locus_tag="I9573_001125"
     CDS             19571..20959
                     /locus_tag="I9573_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAT4795753.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSESAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            21339..22043
                     /locus_tag="I9573_001126"
     CDS             21339..22043
                     /locus_tag="I9573_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HAT4795754.1"
                     /translation="MSKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDPVVT
                     FVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLFLDKIKEDTVNIDGFNLIISIEVA
                     LSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDAPVS
                     NSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNVARG
                     IIEEYIKECNIINLNK"
     gene            22179..23030
                     /locus_tag="I9573_001127"
     CDS             22179..23030
                     /locus_tag="I9573_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAT4795755.1"
                     /translation="MKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITVIPQGAK
                     MEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSITSNIITVIPEKSR
                     VEVLQVDGDWSKIVYDDKIGYVFNYFLSTDGNKPNELDYKYFYTDMIKFVNENNIKST
                     SDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRKPSFGTD
                     EYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWIYDNIPD
                     TTTVIIH"
     gene            complement(23185..24330)
                     /locus_tag="I9573_001128"
     CDS             complement(23185..24330)
                     /locus_tag="I9573_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAT4795756.1"
                     /translation="MSTYYVPPINLLGKGCLNEAKDSIKSLGTKKAFVVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVDKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIVAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAVEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKEKIIELFMKIS"
     gene            complement(24663..25439)
                     /locus_tag="I9573_001129"
     CDS             complement(24663..25439)
                     /locus_tag="I9573_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAT4795757.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
BASE COUNT        10186 a         2725 c         4345 g         8545 t
ORIGIN      
        1 aatagttttg tttaaatata tttttatatt ttgtaatttc aaattaataa ataattagaa
       61 ttgataaaca aaaaataaaa atgttgatta tattaataaa ctttaatata atatatttat
      121 gaaaaatgta aattgtgaaa tggaggtagt aacatgaaaa ttgaaaatct ttctattgat
      181 gatattaaac gaggctatat atttaatgat gatttaaatt gttatagttg cattaaatgt
      241 acaaaaacat atgaggtagg agaaatatat aaaataggta atagattctt tgaagcttca
      301 aaagctattg aacttcatat ggaattagaa catagtgatt atttagagca attgcttaat
      361 agtgattcaa agtacaacac attgactgaa aatcaaaaac aattatttca attatttatt
      421 agtgatttac cagataagga gatagcaaaa caggttggag tatccacatc tactatacgc
      481 catcaaaaat ttattttcag agaaaaagca aaacaggcta agctctattt agcactatat
      541 gaatctgtat ttgaagataa atctaataaa agtagtacta taataccaat ccatgaacat
      601 gctcgaatgg tagatgaaag atatgtagta actgaagaag agagaaaaca cattttggat
      661 acatcatttg aaagtatgaa tcctttaaaa ttaaaagtat tttcttcaaa agaaaagaaa
      721 aaggtcgtaa ttttatctaa gattttggaa caatttgaac gtgggaagaa atattcagaa
      781 aaggaagtta atcagatttt agaatctata tatgatgatt ttgcaacaat tagaagatat
      841 ttaattgagt atggctttat gagtagaaat aaagaatgta caaagtattg gttaacataa
      901 ttaaaataat aaattttact aatgtgtaat gttattttta tctagggggg aggagttaaa
      961 tatgaatgat gaattaaaat ctattttata caataaaggt gtagacattg ttcgctttgt
     1021 tgatatatct gaatttccaa taaatcaaac acaaggtttt tctaaagcaa ttttattttg
     1081 tatagggtta tcaaaaaaat ttattacaga tatatacaat aacttaccta cagatagtga
     1141 tgagtttttg gaaaaagagg agaaagtaga agaattagct gactggattt caaaatatat
     1201 tcaaaataaa ggatatagag cgtattctca atcggagaaa aacaatctag aacatggata
     1261 ttttgaaaaa gcctatatta atccagaaat gcaatcaggg attagtcctt taccacataa
     1321 gacaatagca aatattagtg gcattggatt tatgggaaaa aataatttgt ttgtaacaga
     1381 ggaatacgga tgtgctttta gcatgtgtac agtacttaca gatgctccaa tttctgttga
     1441 aagatatcct ttgattgatt caaaatgttt aaattgtaat gtttgtgtag agaactgtcc
     1501 tgcaaaagct atacatggaa atgaatggac tttacctgga aaaagagaat ctattattga
     1561 tgtttcaaag tgtttttgtg cattgaggtg tatgatgagt tgcccatggt cattaagata
     1621 tgcaaatcaa aaataacttt actagagaag gtattaaatt gaatattata aaatttttaa
     1681 aatgtaagat tatataagat ttctcaaaat aaaaatttgt atagaacact cagaaatgag
     1741 tgtttttcta ttgggattaa attatattga tacaaagctt aactatttta atcattatat
     1801 aaaatttgta ttaaaaaatt aagaataact tgctaatagt aaaaatataa tgagattata
     1861 attgatttat aatataactg taatgatgtt gtaaaatgaa aattgtcatc tcaaaattcc
     1921 caaaaagagt aaaagtcact tcggttatag tcaaaaaatt ttttctgtga aaagaattta
     1981 gatttaaaag ggaggaatta atcatggaaa ttgaaactaa aactccagat tggattaatg
     2041 tactagaatt aaacagtaaa atagatatta cttggaaata tgatgtaagt aatatgatac
     2101 aagatattgt ctctgataag tcactagaag gttccattat atattttcca aaaggtaatt
     2161 acttatttga gaaaggtatt aaaattagtc aacaaataac acttcaagga gattcttatt
     2221 atggaggaga taatcaagta tcaaatttag ataaaaaaca atcaatcgta ggaacaacca
     2281 attttataac ccgaggagtt tctaatataa gtattattac attggaagga acaagtcaat
     2341 gtatcaaaaa tattaatttt tattatgata gtcatgatat agagaaaata ccacctaaaa
     2401 atgtatctgc tattacagaa tatggagaaa cacaaggact atttcacttt gaacacctgt
     2461 ttatttctgg attttcagga atagggattg aaattccata ctattcaact ggcaatgata
     2521 tcattgttag ttcatgtggt cttggtatga ggcttggaga gaagtcaatg ctttctagta
     2581 gcaagattta tgaatgtaaa aatggaatgg agattactac tggtgttagc ttaaataatg
     2641 tgagaataga agaaataaga gaaataggta ttaataataa aggatttggt tttaacctga
     2701 ttatgaatct tatggtcgac caatgtggat attgtggatt tatgtttgaa aaaatgtgtt
     2761 atagccagat aactgccaga attactaaat gcggtcagta ttataaaagt gttgattata
     2821 atacttatga gaatatggaa gatagaaaag aagaagctta ttccattttt tatggagata
     2881 ttttagaaaa ttgcaatatt gtattgatga atagtaatat tgatacctta gaaaatgatt
     2941 cacttgatga acataagata tatgtaatca aggcaaatga aacaaacaaa gtaatgttaa
     3001 catgttatgc tgaagcagat gagcttatac agtgcgcaaa gggcaacttg ttgctagaaa
     3061 atggtgaaaa tacttataaa ttttataatg gtgagatttg ttctgtagga ggtgttcaaa
     3121 tttctgatat agatgaaaaa gatttaatca aaataatcga gaacactctg tatatagatg
     3181 ataaaggtaa gtacttaatg gttcctagat taaaaaagaa tactgtaata tattcagtac
     3241 tagattctat agaagaaata aataaattac atggtggaat atgggaacag attgatgtaa
     3301 aagtattagc tggggaatct atgtactatt ataaaaagat aaaagattaa attatcaaac
     3361 taggaggtag tgattaatgg aagagtctat aataattaaa gatataagtg gctggattaa
     3421 tgtacttgat tatgggattt gtaatgatga aacaaaagat gtaagttcca agttgcaaga
     3481 gattatagat gctgcaccag aagattctat tatatatttt ccaaaaggaa aatacttatt
     3541 tgaaagtggt gttaagatta agaaacgatt aaccttatgt ggagattctt atattttggc
     3601 gaatgttcct aaaaacactg ctggagtaac acaatttact tttaaaggaa agaaaaatcc
     3661 taaaagtgaa aatatagcaa ttataacagc aaaaagtgta agacactgta ttaagagcat
     3721 ttgcttttat tcagatagtt gtgatagaga tatgacatct aatcatgaac ctccaacaga
     3781 tggaaaacct agatttcatc ataatgtaat tataaattat agaaatatat cggctattgt
     3841 atgtgaagat aaacagggag aaccaggtca ttatgaaaac ttgtttttta gtggtttttc
     3901 tggaattgca cttgatatgc ctaataattc tacagcaaat gatattacag tttttacatc
     3961 cggacttgga attagtacag gggaaaattc aacgattgct aacagtaagg tttggggctg
     4021 tgttgatgga atgataattt ctactggaac ttttataaat ggtatgaggg tagaagaaat
     4081 aaaaaaagtt ggaattaaaa atattggtag aggtttaaat tttattacaa atatcacaat
     4141 tgaccaatgt ggatattgtg gagtttggtt tgattctata tccaatgttc agatatcagg
     4201 aaatatcaca aggtgcggac agtactatta tgatgtaagc tatgataatt atttaatgtt
     4261 ggataataga gttaaagaag catattcttt attctatggt aatttattgg agtcaagtaa
     4321 tatagttttg tcaaatacca ataaagataa ttgggaagat ttcaaagagg ataagcataa
     4381 ggtgtatctt gtagaagcac ttgaaaccaa gaatgtacta ttaacatgca atatagatgc
     4441 tactgactgg attgttaagt cagaaaaagg aaatttaacg tacaataata tgcgacaaac
     4501 tgttatattt tacgatggaa aactaagtag tatcaatgga attggtatat ctgatgaaga
     4561 ttatggggat aaagtcaaga taaaaaaagg aaatatatat ataaatagta aggataaggt
     4621 tattaagatg cataaaccag aggtaggtga cattatgtca acaattatta aagatgaaaa
     4681 tatactttcc gctcattatg ggggaacatg ggggaaaatt ggtgaaaaaa tagagtttgg
     4741 aataactgta cactattata aattgataga gaaataagta aatacgtagt atttgaatag
     4801 atgtatttta ttggagagct aggactatag ttttaatgta aatggtttgg taatagcatg
     4861 gattgaataa tataaaaata atataaaaaa ttaatttgat tatgtaaaat tctatgaagt
     4921 agtaaacctg tgactttagt catggtaggt tcaaattaag taaatgggaa aggaatatat
     4981 aatattatgg aaggaagaac tgggattaaa gatggaagat ggattaatgt agtagaaaga
     5041 ggtattaaaa atgatggttc gtacaacata ggtcctgatt tacaggatat tattgatgaa
     5101 atagaaacag atttctgtgt cttatatttt ccaaaaggaa aatacttatt tacaagtggt
     5161 gttaaaatta ataaagaaat tgtattacaa ggtgattcta attcacctac agagactaca
     5221 caatttataa ctcgtggagt agaaaatatg agtattatta cattgacagg gaagaaacaa
     5281 tgtattaaaa atataaattt ctattctgat agttgtgaga tacaggtaaa tgaagaacca
     5341 ccgacaaaag gtaatcctaa atatcattat gagatgatta ttaataagaa tgaaaaaaat
     5401 gttgagatga ataatgtatc agcaattctg tatgataata gtaagaatat gattaaaggt
     5461 ctaggtcatt atgaaaatat ttatataagt ggtttttcgg gtacaggaat taggatacca
     5521 tattactcaa ttgtaaatga tattacagtt tcatcttgtg ggttgggtat tgatacagga
     5581 atagacacaa ttatttctaa tagtagaata tctaagtgtc aaaatggaat gaggattaga
     5641 actggaacta gcataaataa tgtaagaatt gaaaaaatac agaaagttgg tcttataagt
     5701 agtcaagtta aggattatga gggatatgga agttataaaa ttaataatat tacgattgac
     5761 caatgtgggt attgtggttt tttatttgac tcagtgaaag gtagttattt ttctggtatt
     5821 ataagaagat gtgggcagta ttattataat acagattatg acacatattt aagtattaaa
     5881 gatagagtag aagaagcata ttctttattt tatggaaatt attttacaga atgtaatatt
     5941 gatttagtta gtgataataa ggataactgg gatgatggct tagaatataa acataaagtt
     6001 tatgtattta aaacccttca aataccaaaa ttaacattga gatgcaatat agatgcctat
     6061 gattatatcg ttaaatcggt acatggagga ggtaatttaa tgttaaaaaa taactttaac
     6121 aattatatat ttattgattc atctagtcca gaagttatta atggaattaa ttttaattat
     6181 caaaagaatg gaaacctgtt aaggttaaat aatggtaaat taagttataa aaatggaaag
     6241 gaagaaacag ctatattcag acctaattat ggtcttattg tagcatcaac agattcagaa
     6301 tgtgaacaaa tagagactag ctatggagga aaatgggaga agcttggttc tgaagtaatt
     6361 ggaaaagaaa cggtatatta ttataaacat catgaaccag ataaaacaaa ccaaataaaa
     6421 taaaatttgt attatataat gatataaagt tttaatatat agtgtttacc tttaatgtaa
     6481 tattggtata tttgtatagt attaaatttg ttaaaaagta aaattgtagg gtgcttgaaa
     6541 atagcacctt ttttagtttc attataaaat tagttttgaa agtttaggaa taatgtatat
     6601 ttatttttaa agaattataa ccttattaag gatattttaa atgctagaaa tgaatcagta
     6661 aatcatactg gaattatctt aaatgttttg attaaattaa gtatttagag ctataattat
     6721 attaaaaata tattaggaca taattatatt actatggagg ttaaatatga tagtaagtgt
     6781 gagtagaaga actgatatac ctgctttcta tagtaagtgg ttttttaaca gacttaaaga
     6841 aggttttgtt tatgtaatta atccaatgaa tccaaaacag gttagtaaga tagagttaaa
     6901 tccacatact gttgattgtt ttgtattttg gacaaaggat gtaactccaa tgttatatga
     6961 tttaaataaa ttaaaagact ataaatatta ttttcactat actataacat catatggaaa
     7021 agaagtagag acatgtattt tagataaaag aaaggtaata gattcattta aggaattgtc
     7081 tagaaaaata ggcaaagaaa gagtaatttt aagatacgac ccgattttct taagtgaaaa
     7141 gtacacagta gattatcata tcaaagcatt tgcgagttta tgtaatcagc ttgatggatt
     7201 tacagaaaaa tgtataataa gttttatcga tttatataag aaaacaaaat ttaatactaa
     7261 agcattacat ataaagccaa tagaaattac agaaatagaa acattatcaa aagaaatttc
     7321 taagattgct cataagcatg ggataatact tgaaatgtgt tctgaagaat ataatttaag
     7381 taaatttggg attggaaaaa gtaagtgtat tgatgataga ttaatatcaa aaattatagg
     7441 atgtgaagta gaggtaaaaa aagatagtac acaaagagat atttgtggat gtgttaaaag
     7501 tgtcgatata ggacaataca atacttgcag acattattgt ctgtattgtt atgctaactt
     7561 taattatgca aaagtagaag aaaactgtag gctttataaa gaagataata aattactcat
     7621 agatacttta agagatgatg caaaaataag cattagagat atgaagacta ttaaagtaga
     7681 taagaataaa caaattagta tatttgatta aaataaatat actaattacc ttaatatatc
     7741 aagaatatag atacttttga taacaaaatt aaacttaata gatattttct atataagaaa
     7801 gatggtcata atatgtcaat ttgaccatct ttttgatata attaaaacac aaataattta
     7861 gtaacaaagg aggcagaact tgaatttatt aaaaaaatat cctataccta ttgcaagttt
     7921 gattttgtca atttttactt taggtaactt attacaatct tatagtgaat cattacgtaa
     7981 tgtaattgga tttataggat ttattttgta tgcaatttat atagtcaaaa taatattact
     8041 tagaaagggt gttaaagagc aattagagaa ccctttaata tcaagtacat ttccaactat
     8101 aacaatggca acaatgatat ttgctacata tataaaacca ttatctttag aattaagtat
     8161 tatgatttgg agcataggaa ttgtgggtca tcttgtgtta atagttttat ttagtaagaa
     8221 gttcttagtt aaattctcaa taaaaacggt atttccatct tggtatatag tatatgttgg
     8281 gatagttgca gctagtgtta cggcaccagc atttaatcaa cttttgattg gcaaaattgc
     8341 tttttggata ggatttgtat cttatatagt tcttatacca gtaattttaa agagagtatg
     8401 gataatcaag gaaatgcctg aaccatcttt accaagtata gctatattag ctgcaccagg
     8461 gtcactatta ttgtctggat atattaattc tttttcagat aaaaataatg tagtattata
     8521 cttgttattc atattatcaa ttgtatttta tattatagtt ttgggatact taccgaaatt
     8581 attaagattg cctttttatc cgagttttgc tgcatttaca ttccctatgg caataagtgc
     8641 aatgggtatg aaactaatga atggttattt aactaaatca ggtcatttta taagttggat
     8701 ttcatatgct tctaagttag aagagataat tgctacagtg attattttat atgttttagt
     8761 tttatttatt atatatctta gtaaatctga aactacatct aaagctaaat aagaattatt
     8821 aaatatgtgt atttaatagg aatattgata taaatatatg aagataagat aaaccataga
     8881 aacttaattt agcttaattt tattggagga aaacaaatga aaacaaaaga agaatattta
     8941 aaagaagtta gaaacatatc agaagaatac tttagaaaag gagagttttt ttgctctgaa
     9001 gcagttcttc agacaataaa tgatgcttta ggccaacctt taagtccaga gataactaaa
     9061 ttagcatcag gatttccgat aggacttgga aaagctcaat gcctttgtgg agcgatatct
     9121 ggaggagaga tggctttagg tatagtatat ggaagagtac atggagaaac tatgaatcca
     9181 aagatgtttg agcatgctaa aggtttgcat gattatatta aaaaagagta tggtgcaaca
     9241 tgttgtagag ctattacaaa gaaatgggat ggtgataact ttatgtctcc cgaaagaaaa
     9301 gagcactgta ttaaaataac aggtcaagtt acagagtggg ttgcagataa gcttatagaa
     9361 gatgaaaaac ttaatatcaa atagtgtaat attagttggt gaatttataa agttaaattt
     9421 acttatagta taagatactt gatattctcg tattgataga aaaatttata gcatatgtat
     9481 ttaatgaaat atacttatat ttagagtgtg gtttaatttc ataaatacat atgctattat
     9541 tttaagtata taagcaaatt tatttaaata aatagaaaca gttaatttat atgtacaaaa
     9601 ctatatatga ttagtgtaaa taaataccta ttttataata ctcaacataa agacaccaga
     9661 catgattagt attataccaa taaaaatagc aagccattct tttttgttct tcttttcttt
     9721 aaaaaatata atccctccta aagtggctac tatcacacaa ctctgagaga tagaaaaact
     9781 tattgtaact cccaatgtat tagtcgttat gaacatacct aaatttgcta ttgaccacaa
     9841 tataccagta attaaattga ataaaacatt ttccctatat acaatctctt tagaaaaata
     9901 cttgacaata atagatgaag ttatcaacat accaacagat tgaggtaata taattctata
     9961 tccttcaatt aaaaatattt gattagttac aacatataat gataaaaata gagatgataa
    10021 taatacaact ccaacacatt ttaacatatt tttatctgta gattttttag tttgataatt
    10081 agtgagaagt ataccaagta ttatacataa tattgataaa gaaccaatca gtaacatttt
    10141 agaactggtc cattcattaa acaagattac agctaataat gtcgtaaaag ttaattgtga
    10201 accatttgaa attggcatta ctttagatac actagcaatt tgaattgctt taaactgcat
    10261 taattgtccc aaactccaga atatgccaga aatcatactt atacaaaatg aaaaaaatgt
    10321 aatttcagga tgtataacca gagttattat agtggaaaat ataaacgcac ttatggatgt
    10381 acctaaaagc tgttcatgag gagaactttt ctttagattt gcaactatgg gcatcaatcc
    10441 ccatcccatt acaggaagta aagcaataat tattgtcagc attaaactat ttcctttcat
    10501 attaaattta gacagctttt aatttatcat ctaaaattaa agtgttcaat acataaaaat
    10561 taccgtattt aatcaggaaa tattagtagt tttttagaaa aacattttcc attaaaataa
    10621 agataaaggg aaggagtaaa attgaaaatg ttaactgata tgattcaaaa tcgtgaaatt
    10681 aaaataataa agtttttatt aaaagaaggt catacaactg taaaagcaat agcagatgat
    10741 ttagatgttt cagaaaaaac agtttcaaaa tctcttaaag aagtagaagc agtgcttaaa
    10801 aagataggtc tttctttaat aagaaaacct aagataggtg tttatattga tggagattta
    10861 aaaaaaatac ttccttacct agataataaa ggaagtcaaa ttcctacaac cagagaggag
    10921 agagttatct atatatattc agtactttta aagagtaatg attatattac tatacaacaa
    10981 ctttctgatg aactatatat aagtcgtgga actattgaaa gggatttatc agaagttgaa
    11041 aaactgctaa aaaacgaagg gatttccata tataagaaac ctagtaaggg aataaagtta
    11101 aatataagtg aaagagataa aagacaactt actgctaagt ttatacataa attttggagt
    11161 agaaactggt atattaagca agagcaagaa agcttttatc aagcatttga aaagatacag
    11221 gcagatgtaa atggtatttt ttcggaagat gatttatcaa taattattga tattcttagg
    11281 aatttcagca gagaaagaaa atttgagttc tctgattatg aatttcagtc attagttatt
    11341 catttggcaa tagctgtaga gagaattaga aaaggcgaat atatagaaga aagtttgtat
    11401 attggtaata tagaaaataa tcaaatgtca aataccaatt atttagcaaa acaattagaa
    11461 gaaaaatttt caatattatt accaaaatct gaaattggat atataaattt acatttagta
    11521 gcggctgacc aacatagaga tactaatgaa ctaaaaaata cttcaactaa tccttgtgaa
    11581 acaagtacaa ataatatatc aaatcttcgg gaaattataa aaaatacatt gtatgagaca
    11641 gcttatgaca aggatttaat tgatggattg acttcacatg tacattcatc aataaataga
    11701 ctgaaatatg gacttagtat taaaaaccca tatgtaaata caatcaaaca aaacttttct
    11761 ttatcatttg agcaagcttt agatttaaag aaagtaattg aatttatata tgatattaga
    11821 ataaatgatg atgaatgtgc ttatatagca ttacattttg aagcttttag agaaagaatg
    11881 aaagaacttc cacaaaatat tagagtaatt ctagtatgca gtacaggatt aggttcatct
    11941 aggttattgt ctgcaagaat aaaaaaatac tttccaatga taaagattga aaaagtactt
    12001 tccgtacaag aattagtatc cagtaaaatt gatgcagatt tagtaataag tactatttat
    12061 ttagagttag aaacaattcc aaccatagta gttagcccta ttttaaatga aagtgatata
    12121 agaaccttgg aaagaaatat acaaaatgtt tcatgttgta cttctaagaa gtataaatct
    12181 ttcagaaacc taatctttga agaaaatata ttgttaaata ttgaagcttc aaactatgaa
    12241 gatgcaatta agtatataac agataaccta ataaaaaaag gttttgtaaa agaaggtata
    12301 gctaaaagtg caattgagag agaaaattta tcttacacgt cttttcagtc aatagctaca
    12361 cctcatgcta atcctagtta tattaaaaaa tctaatatat ctgttgcaac gttaaaaaat
    12421 cctattacat ggggaaatga aaaggtggaa gttattttct ttatttcatt agaaaatgac
    12481 aaaaacctag agcttgatga aatatatgaa gttttttatg actttattga caataaaaaa
    12541 aatataaatg acatattaag tgcaacttcc acaaaggaag tgtacgattt gctattggag
    12601 gagagtatat agtgaaaata tcagatgtat tgaaaaaaga gcaaattgtt tttaatcttg
    12661 atacaaatac aaaggaagaa acaataaaaa aattaagtag tatatttgtt gaaaatggga
    12721 ttgtggacaa tgaagaagaa tatgttaagt ctgttttgga aagagaagaa cattcaacta
    12781 caggtattgg taatggtatt gctattcctc atggaaaaag tgatgctgtc aaaaaatctt
    12841 ctataatatt tgcaaagact aagcataaga ttgagtggga tgctttagat gaaaaaccag
    12901 tagatagtat tttcttattg gctataagca atattgataa ggatagcaat catcttgtac
    12961 tattatctaa attagcaact aaacttatgg atgatgacaa tgtagatgca ctaaaattag
    13021 ccaattcaga acaagaaata atcaatatat ttagtgaaga aggagagatg tagaaatgaa
    13081 aagaaaaatt atagcagtta cagcatgtgc aacaggagtt gcacatacat atatggcagc
    13141 tcaagcgtta aagaaagctt caaaaaagat gggtcacctt ataaaagttg aaacacaagg
    13201 agctacagga atcgataatg agttaactac aaaggatgca caaattgcag aagtagtaat
    13261 atttgcagta gatactaaag tcagaaatgc tgaacgtttt gagggaaaag aaatactaga
    13321 agtaccagtt aatgcaccta taaaggatgc agagaaagta ataaatgatg cattaaagtt
    13381 aattgacact aaataaatgg agggaaatgt aatgttgaaa aagatagtct ctgagttaaa
    13441 aaagcatgtc ttaactggaa tctcatatat gattccatta gtaattgcag gtgctatgat
    13501 aatggcaata tctcgtgtag gtggttcttt ctataatata ccagatatat gggatgctaa
    13561 atacgcagaa agtgctagta gtattgttag attattacat gatctagatg gatttggagg
    13621 tactgcatta ggattaatgt ttcctgtaat tgctgcattt ataggatttt ctatagctga
    13681 taaattagct attgtaccag gtcttgttgg aggtatgatt gctaaagata taggtgcagg
    13741 atttttagga gctttggcag ttggtcttat agcaggatat acatgtttgt ttattaaaaa
    13801 tcatgttaaa ttaccaaaat cagcagcttc aatagtacca atatttatag taccagtatt
    13861 tggaacatta atcactgttg tattaatcaa ttatgtgata ggtatacctt ttgccacatt
    13921 aaatactgga ttagaaaatt ggttaaatgg attatctggt acaaatcaga tattaatggc
    13981 agctattata ggggctatga tggcagttga cttaggtgga ccagttaata aagcagcttt
    14041 aacaacatca cttgcattat taacaagtgg aatttatgca cctaatactg ctgcaatggt
    14101 aggaatagtt ataccaccac taggattggg acttgctact atactagcaa aacaaaaata
    14161 taataaacag ttacgtgaag ctgggaaatc atctcttata atgggtttaa ttggagtaag
    14221 tgaaggagca attccttttg cggtagaatc tccattaaaa gtaatacctt catgtgtaat
    14281 tggaacagca attgcttcag ctatggcagt tggtttaggt tcagtaaatg caactccaat
    14341 tagtggattt tatggatggt ttacagtaga gaattggcca atgtatgtgt tatcaatagc
    14401 agttggaaca gtaattgtag ctggattagt tatagtattg cgtggaaatc cagtgattga
    14461 ggatgatgaa tttgaagatg atgattttga tgaagcagag tgggaatctt aaaatttaat
    14521 aactatttaa gacgtgttat aagctatatt caatatgaat cttatactaa ataaagtgtt
    14581 aaatattcag tttagactta aatataaaag ataaagaaca gaggagaata aatgataaaa
    14641 gcacatattg ttaatcatac acactgggat agagaatggt attttacttc tggagatgca
    14701 ttagtactta gtgaacaact atttacagat gtaattgatg aattagaacg taatccagat
    14761 gtaagttttg tacttgatgg acaactttct attttggatg attatgtaca attacacaaa
    14821 gaaaaaatag aaatcattaa aaagttaata aaagaagata gactacatat tggaccatgg
    14881 tttactcaaa ctgatgcatt ctttgctaga ggagagtcta ttttaagaaa cttaatgatt
    14941 ggaatttttg aaagtaaaaa gtatggtaag tatatgaaaa ttgggtattt gccagataca
    15001 tttggattta atgcacagat gcctatacta ttaaagcatg taggtcttga taatatattc
    15061 ttttggagag gtatacatct tggtaaacat gttcaatcac catattttaa gtggaaaagc
    15121 ttaaatggag aaagttatat atatgcagta aatatgccac atggatatgg aactggtatg
    15181 cttttagagc cttcattaaa gtatgttaat ggacgcttag acccagctat tgattttatt
    15241 aaatcatata ctgatgtaga tgaggtgtta ataccttctg ggaatgacca gttaaatata
    15301 ataggtgatt tcaaaaataa aattaaagcg attaatgata tcggaaagta ttcatatata
    15361 acgagttcat atcaagagtt tttaaaatat ataaagtcca tagaatcact agaaagctat
    15421 agaggtgaat ttagagaacc tgtattagca agaatacata aatcaattgg ttctagtaga
    15481 atggatataa aattagcatg tgataaacta gaaaacaagc tgattaaaag aatagagcca
    15541 ttattagtaa ttgctaaaaa gagtaaaatt gaaataagca atcagttact tataaataca
    15601 tggaaaaaac ttttggaagg acaagcacac gatagtctag ctggatgtgt aactgatact
    15661 gtaacagatt atattcttca ccgtataaga gaagcaaatg aaatatgtga cagtattgaa
    15721 aatactattg taaaaaagat ttcagaaggg cttaaattat ctaaaaatga tattttagta
    15781 tttaatacag aagctaagcg ttttaatgga tataaagaga tacaagttgt aagtgatagt
    15841 aaaaatattt attttaaaga taatttagat gctactataa ttgaagaaac ttatgtaaaa
    15901 ccgagagaaa atgtattaga agaaacacct gctggcaacg tatttattga agaatcagga
    15961 tattatatat taaaagtaag aataaatgta cagcttcctg cacttggtta taaagttgtt
    16021 agttttgaat caagtgataa agaaatggta tcacttgata aatctaatga cacttttata
    16081 tctaatgata attataagat aatctatgaa gatggaagct taaatttaca acttaaagat
    16141 ggaacttaca tatcaaactt tttaatatta aaagatagtg gtaatgcagg cgatacctat
    16201 gacttttctc cattaaaaaa tgacgaggat ataaaacttt catttgatag agtgtataca
    16261 gaaaaaactt taggatatga aaagttggtg gttaaaggta ctactttact accacttaca
    16321 ttagaagata gaaaaaacaa atatcttaat gggaaattaa atgtaaaagt aagtattgtc
    16381 ttatccaaag aaaatccatt gatggatgta aaactttgtg ttgataacac gatatataat
    16441 catagactac gtgttcatat aaaaactgat ataaaagata ataaaaacat agcatcattg
    16501 ccatttggat atataacaag agaaaatgga gttttagaca actgggaaga tatttacagt
    16561 gaaatgccta tagatttaga acctttggaa agtaatatta ctctaacaaa ccaaaataga
    16621 agctgtactg tatttactag aggaataaaa gagtatcaac atattgaaga tgaaattgca
    16681 ttgaccttac ttgctactac agatgaacta ggaaagcctg atttattata tagaccagga
    16741 cgtgcttctg gtgatacaac taaaaaaggt catataagaa ttaaaacaga taaagctcaa
    16801 atgcttaaga aattagaatt ttcatttgca atatatatgg attctaaatc atttgatgaa
    16861 ttggaaattg cgaatttaac ccacaattat ctaagggaat gtgtaaatta tcaattacag
    16921 gattacaatt tctttttata tcgtatagat aataagattc aaaaatcaat tgtcaataaa
    16981 actgtaagta gagaattaga aataatatct ttaccagaaa aatatcttat atcagcatgt
    17041 tatccttctt attacagtaa agataaattt ataataagag tggaaaatcc aagtagtaat
    17101 agaatgatat tggatgagca aatatttaaa aatagaaatg ggaaaattgt aaatgcaata
    17161 gaagatgtag aagaagaaca ggtatatgaa atagcacctt ttgacgttat ttcaatatta
    17221 ttggatttat aagtgtaaaa gtaagtttat tgtttaaagt ctgacagata ttaatctgcc
    17281 agacttttta atttgtataa atttgggtaa atattaatga atagttttag tatttaaata
    17341 tgaatttagt atttaatata gaatttattg aagtagaata attagtttaa caggataagc
    17401 agtttaaata aagaattatt atattagagt gattttatat ataatttaaa ataataaaat
    17461 gttaataaaa tgagttaaag attgtatatt aatagggttg atgtagttac aaaaaagtta
    17521 ccaattgtta tgtatttaca aaactgatat attaattaca taaaatctaa aatagtttgt
    17581 aataggagta tatataataa aaattttaat taaataaagc taggtatatt tataatatta
    17641 ataaatatat tgctataaaa gtttgtttaa taaattaaat aaagaaaaga ggagaataaa
    17701 atgtacaaca tagatgatat gatatataat tgtccagtag aagctttatc aaatatttta
    17761 ggtaaaaaat gggtggctat aattatatgg aaaatacaag aagataagaa acgatttgga
    17821 gagctacaaa gagctatacc agactgtagt aagaaaatgt tagttcaaca actagatttt
    17881 ttaattgaac aggggattat agtcaaccaa aaaaagttgg ttaataatgt tgtagagtca
    17941 acatattttt taagtgaatc aggattacga ttgcttcctg tcatggaaaa aatgattatg
    18001 tggagcaata aaaatttaaa ttgtgataaa tactaaaatt tactctgtaa gtaatgaaaa
    18061 tttaattaag ctatgagtag aaaaatgtat taaaattaca taatttttta gttagatttt
    18121 aatttagaaa tgggattgta atattggaaa taaaataagg aggaatttta atttgcaaaa
    18181 tgtaaattta tttttagtat ttatagaagg tatagtatca ttcttttcac catgtatact
    18241 gccaattttg cctatttatt tgagtatatt gtcaaatagt agtgtagaaa atctaaaaga
    18301 aggaaagacg agttttatag gaagttcttt atttaaaaat actatatttt ttgctcttgg
    18361 aatctcaaca acattcttta tattgggttc atcagtaaaa gtattgagta tgttttttaa
    18421 tgaaaataaa gatttaataa tgtttattgg tgggattata attattatca tgggattatt
    18481 ttacatggga ataattaaat cttcaatatt gaatagagaa aaaagattta atgttaagtt
    18541 taaagaaatg aaagcaataa cagcttttat attaggtttt acgtttagtt ttggatggac
    18601 accttgtatt ggtccaatat tggcatctgt attagtcatg gtttcaagtt caagtaatca
    18661 tcttagtgcc aatcttttaa tagcagtata tactattggt tttattttgc catttataat
    18721 tacagctatg ttttacagta aactatttaa aactatagat aagataaaat ctaacatgga
    18781 aattattaaa aaaataggtg ggattatact tatagtatca ggaatattaa tgatggtaaa
    18841 tggatttgga agtataagca aacactttaa tacgtcacaa aatagcaaaa ttgaaagcaa
    18901 gcaagaagaa aataagcgag aaaattctac tgataaagaa gaaaattctg atggtaataa
    18961 tagtcaaaaa gactctaata atgacaataa tgataacggg tctaatgatg aagatagaat
    19021 aaagtctatt gattttactt taacagacca gtatggtaaa acacataaat taagtgatta
    19081 tgaaggcaaa gttgtattct taaatttctg ggctacatgg tgtcctccat gtaaagaaga
    19141 aatgccttat atagaacaac tgtataaaga ctataataaa aacaatgatg atgtagtaat
    19201 tttaggagta gcttcaccta atttaggtag agaaggttca agggaacatg tagttaactt
    19261 cttaaaagac caaggttata cattccctgt tgtacttgat gaagatggag cattagcata
    19321 tcaatatgga attaatgctt ttccaactac atttataatt gacaaggaag gatatgtaac
    19381 tcagtatatt ccaggagcta tggataaggc aactatggca tcatttattg aaaatcagag
    19441 aaataaataa aagtatttaa gtatatgttt atgaatcaat ttaaggttac aaaaaagtgc
    19501 ctaattgtac cacctaaata atcttgttat agtaaaagta taaacaatac aagaaagggt
    19561 tgaattttat atgaaaaaaa catttgatgc tataataata ggatttggaa aaggtggaaa
    19621 aacattagca ggagatttgg caaacagggg attaaaagta gctttaatag aaaagtcaaa
    19681 caagatgtat ggtgggacat gtgtaaatgt ggcatgtata ccaacaaaaa gcttagaaaa
    19741 ttcagcaaac agcgtaaaaa caaagaatat aaattcttgg gatgaagtac aagctgaata
    19801 tgagaaagct atagataaaa aagaaacttt aattacaaaa ctcagagaag ctaactataa
    19861 taaattaaat tcaaatgaga atgtaactat attcacagga atgggaactt tcattgatga
    19921 aaaaacagtt caagttaaaa ctgaaaatga aatttatgag ttagtagcag ataatatatt
    19981 tattaatact ggttcaagac catttatacc taatattaag ggaattgaaa ataagaatat
    20041 agtatatgat agtgagtctc ttatgaatct aagagtttta cctaaaaaaa tgacaattat
    20101 aggagctgga tttataggtt tagagtttgc tggtatttat agttcatttg gagcagaagt
    20161 tacaatatta aactctaata atggcatttt accaaatgaa gatgttgagg attcagaaga
    20221 aataataaaa ttacttgcaa agagaaatgt aaaaattgtt aataatgcaa atataaaaga
    20281 gattaaagaa gtttctgaat cagcaatagt agaatatgaa gtagatggta aatctaaaga
    20341 attaacaagt aatatgatac ttgtagctac aggaagaaaa gcaaacactg aagggctagg
    20401 gctagaaaat gcaggtatag aattaaatga aagaggattt attaaagtct cagaaactct
    20461 taagactaat aaagagcata tatgggctat aggagatata aatggaggtc cacaatttac
    20521 ttatatatca ttggatgatt atcgcatagt tattaatcag ttatttggag ataagactag
    20581 aacaactaat gatagaaaga atatcccaaa ttctatattt ataagtccag cattttcaag
    20641 agttggtctt aatgttaaac aagctaaaga aaagggatat gaagtattag tggcaaagat
    20701 gccagtagaa gcaattccaa gagctaaaca gattggcaag gcagatgggt ttataaaaat
    20761 agtgatagat aaaaaatcca ataagatact tggagctagt atgatatgtg aaaattcaag
    20821 tgagattatc catcttattc aattagctgt agatttagaa gtagagtata catatttaag
    20881 agaccgtgtt tatgcacatc caactatgac agaagctctt aatgatattc tatctcctaa
    20941 tatgataaaa gaagtataac ataagtttaa tatataaacc taaaattatc attataagta
    21001 gattttagaa agaggctatc tcaaaaaata gagataagtc tcttttttac tttttaaatg
    21061 ataaaaatat atttgatttt ataaaagaag attatcttat gaactaaaaa gtttatttta
    21121 aaacactacc tatttatgtt ataaacttat aaatataata tagaatctat tattctgtat
    21181 tattaagtga ggttaagcgt tataattaat tataaatatt gaataatcta aatggataag
    21241 ataatggtat taaaaattat cttaaaaggt gaaataatct gtatttaatg tagtacagtt
    21301 atatctttaa agcaaggtgg tgaaatgata taaataagat atctaaaaga gggtttgatg
    21361 atagtgataa aagatggttt tcaagtaaag aattaacaag acttataaag gctaaagaag
    21421 aaatagaatg gttattaaat agggattaca aaatagaccc agtcgttact tttgttggtg
    21481 atagatatca gttttctata agacaaagag atgcattaaa aagagcagtc tgtactgatg
    21541 aaaaaaatat tataagacaa tcaaaaaggc tttttctaga taaaatcaaa gaagataccg
    21601 taaatataga tggctttaat ttgataatat ccatagaggt agcattatct ggtggtactc
    21661 tagtaatagg aagtgatgga aatataagag atttagcagg tcttagggga acctataaga
    21721 ttatagataa aacagaagaa gctttgaatc tgataggtaa attttttaat aaatacaatg
    21781 cacagaatct taaattttat ttagatgctc cagtttctaa ttcaggaaat ttaaagtata
    21841 ggatattaga gcatgccaaa acttggggaa ttgaaactga agtagaatta gtaaaaaatg
    21901 cagatgttgt attggaaaaa ttagatagag tagttagtag tgatgcagtc atagttgaca
    21961 agtgtataag ttattttaat gtggctagag gtataattga agagtatatt aaagaatgta
    22021 atatcataaa tttaaataaa tagataaata gaggtgagaa tatataagta tatgtagcaa
    22081 tattattagt aatacaagta aagtttgaaa ataggagaac gaatacatat cgaatttcat
    22141 aaaatgttgt aaaaagacat atgagaggat ggatatgtgt gaaattaact aaactaaata
    22201 ttaaaaaata tagagcacct atatataaat atgctttagc aaatgtaaat ttgaggtctg
    22261 ctaaatctac taattcaagt atcataacag taatacctca gggagctaaa atggaagtat
    22321 tggatgaaga agatgactgg attaaggtga tgtacaattc tcaagaagga tatgtgtata
    22381 aggatttggt atcagtaagc gaatatgctt ggagtaactt aaacttaaga gaagataaat
    22441 ctataacttc caatataatt acagtaattc ctgaaaaatc tagggttgaa gtccttcaag
    22501 tagatggaga ttggagtaag attgtatatg atgataaaat aggatatgtt tttaattatt
    22561 ttttatcaac tgatgggaat aaaccaaatg aattagatta taagtatttt tatactgaca
    22621 tgattaaatt tgttaacgaa aataatatta aaagtactag cgattatcta attgtaacag
    22681 accttagaaa taaatatact tatattttta agaaagataa tggaggctgg gggcaacttt
    22741 acaagtggca atgtactata ggcaagcctg agactccaac tataacaggt atattttata
    22801 taagtggaag aaaaccttct tttggaacgg atgagtattc agttaagtat gctactagaa
    22861 ttaaaggtgg atattactat cattctgtgc tatatgattc aacaggaagt tatataatag
    22921 atggcagact tggtgaagct cttagtcatg gatgtataag attaagtacg gaaaatgcaa
    22981 agtggatata tgataatata ccagatacaa caacagttat aattcattaa gaaaaatttt
    23041 gagtttttat tgggataatg attctcaaat taataggatt atatattaac ttcttcataa
    23101 agagtactat ttaaaaagtg taataagaga gtgctattaa caaaacaaag atattttgag
    23161 gcactctctt ataaatatac ataattaaga tattttcata aacaattcta taatcttctc
    23221 cttatcaaag aaaacaggat tagcaccagc acaagcatct ttcatagagt tttctgcaag
    23281 caattcaaaa ttaggattat caacacctac atcttttaaa gattttggga ttccaacctc
    23341 ttcagaaaga gcttttatct tatctattac aaagtcaaca cactctttat ctgatttatt
    23401 atttacatcc attccaataa cttcagctat tggtcggaat ttggcagggg ctgatttagc
    23461 attttcctcc tcaactattg gaagtagcat ggcattacat actccatgag gtaaatcata
    23521 taaacctcct aattgatgag ccatagcatg tacatttcca agacctgcat tactaaaggc
    23581 aattccattt aaaaaacaag cataacacat ttgttctcta gcttctatgt cattaccatt
    23641 tttaacagct cttggaaggt atttaaatat ctgctttata gcatataagg cagttgaatc
    23701 tgtaacatca taagcaccat tagctactac agcttctaca gcatgagtta gtgcatccat
    23761 accagttgct gctgttaaat tagatggttt gctaatcatt aattctgggt catttactgt
    23821 cattgtagct agtgagttgg tatcaaccat tatcatcttt acatgtctgt cttcatcagt
    23881 tataacatag tttattgtaa cttcagcaga tgtaccagca gtagtagtta tagctacgat
    23941 aggaagacat tttttagaag ttttgttgat accttcatag tctctaatat ctcctccatt
    24001 agtagctaat atagagatag ctttaccaca gtcttgtgga gaacctcctc ctatagttat
    24061 aacgaagtca caattttctg attttaatat tttaagtcct ttatccacat tagttacggt
    24121 tggattttgt ttaacttcat cataaacaac aaagtctaca tctatctgag ataaaatatc
    24181 tgttactttt tttacagtac catttgaaac tagaaattta tcactaacaa caaatgcttt
    24241 tttagttcct aatgatttaa ttgaatcttt agcttcatta agacatcctt tcccaagtaa
    24301 atttattggt ggtacataat aagtgctcat aactaaaatc tccttttatt ttcattgatt
    24361 tttatgtaaa aatatatatc taacaatttt cactaattta ctcataatat atcgtatctt
    24421 agattgagat agggttactg gttcttatat aagaacttaa ttcgtaatta cgattatgat
    24481 tagaatttaa catagtattt ttatcatgtc aataaaaata taaaaaatat tataaaaaaa
    24541 acaaagttac tgtagttaaa tcagcaactt tgtttttata gtataaatca aataaataca
    24601 ttagtttaaa acaaaatttt taattattat gttttgatat cttccatata cagaatatta
    24661 atctaattta ttacaatgtc ttcattacgt tcttccatca ttgattgaat atttttctga
    24721 gcatttaaga gtaatttttt tatcagttct attttttcgt cattagctct aaataaaggc
    24781 agtgttacac taacagcagc tcttatacat ccattttttc ttattggtac ggcaagacat
    24841 tttaggtgtt ccattgattc ttcacactct gaagctattt gtgttctttg aacttcaaga
    24901 agctggtcgt ataaaacatc aaaatctgta attgattttt ctgtaaaacc ttttagacca
    24961 tctggatata agcttattag tttttccttg tcaaaatcac ataaaagagc ttttccaaga
    25021 cctgtacaat tagcaggtat tttttttcca acataagaaa taagtctaat tggttctgga
    25081 gaatctactt ttccaatata aagaacttca ttattgtcaa gtataccaag ctgacaagtt
    25141 tcagaacagg aattaactat attagtcatt tcgtcaatta tatagttaaa aatagttctt
    25201 ttttcaaaaa atgaagaccc taatgtgtaa gaacgtatcc ctatagaata tttggatgtg
    25261 tttttatcaa tagtaatgta ttttctagca ttaagtgtat gaactatagg aaagatgcta
    25321 ctttttggag cattgataac atctgctatt tctgtaagag tatatccatt ttcggattct
    25381 gctaatgctt ctaaaatatc taatatccta gctgttgggc gatgattatc tgaagtcata
    25441 aatttccccc tctttttatt aatttaaagt attagaatat gttgatttta gcacactata
    25501 cgatatttta caatgtagaa aataaatatg taaatttata tataaataag ctcttgattt
    25561 agatacagat agatattttt ttagactgta cattaaggaa taactaattt ccaaaatata
    25621 cctaaagaaa gtaagtgata tttagttata taatttaagt aaatatatgc aatagttatt
    25681 tttatatgta aatatcaaaa tattatgaat ttatatttta tctatttttt acactgtata
    25741 aaaactttat agttttgaat actatataat tttaaatacg ctgtattttt gcatataagg
    25801 a
//
LOCUS       DACTGW010000035        25505 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.035, whole genome shotgun sequence.
ACCESSION   DACTGW010000035 DACTGW010000000
VERSION     DACTGW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.035"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            129..1265
                     /locus_tag="I9573_001130"
     CDS             129..1265
                     /locus_tag="I9573_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAT4795758.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLKPLASGDKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            1324..2106
                     /locus_tag="I9573_001131"
     CDS             1324..2106
                     /locus_tag="I9573_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAT4795759.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            2125..3135
                     /locus_tag="I9573_001132"
     CDS             2125..3135
                     /locus_tag="I9573_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAT4795760.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            3154..3951
                     /locus_tag="I9573_001133"
     CDS             3154..3951
                     /locus_tag="I9573_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAT4795761.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            4049..4894
                     /locus_tag="I9573_001134"
     CDS             4049..4894
                     /locus_tag="I9573_001134"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HAT4795762.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            4981..6156
                     /locus_tag="I9573_001135"
     CDS             4981..6156
                     /locus_tag="I9573_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAT4795763.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            6351..7253
                     /locus_tag="I9573_001136"
     CDS             6351..7253
                     /locus_tag="I9573_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAT4795764.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKKEK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            7267..8271
                     /locus_tag="I9573_001137"
     CDS             7267..8271
                     /locus_tag="I9573_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAT4795765.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(8415..8642)
                     /gene="acpP"
                     /locus_tag="I9573_001138"
     CDS             complement(8415..8642)
                     /gene="acpP"
                     /locus_tag="I9573_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAT4795766.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            8814..9239
                     /locus_tag="I9573_001139"
     CDS             8814..9239
                     /locus_tag="I9573_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795767.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            9287..9493
                     /locus_tag="I9573_001140"
     CDS             9287..9493
                     /locus_tag="I9573_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795768.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            9675..9854
                     /locus_tag="I9573_001141"
     CDS             9675..9854
                     /locus_tag="I9573_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795769.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            9888..10088
                     /locus_tag="I9573_001142"
     CDS             9888..10088
                     /locus_tag="I9573_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795770.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            10341..11357
                     /gene="ccpA"
                     /locus_tag="I9573_001143"
     CDS             10341..11357
                     /gene="ccpA"
                     /locus_tag="I9573_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HAT4795771.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(11480..11878)
                     /locus_tag="I9573_001144"
     CDS             complement(11480..11878)
                     /locus_tag="I9573_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795772.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGGQQKKSKGLFKKTKKSNPHE"
     gene            12090..12995
                     /locus_tag="I9573_001145"
     CDS             12090..12995
                     /locus_tag="I9573_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795773.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            13110..13290
                     /gene="ssrS"
                     /locus_tag="I9573_001146"
     ncRNA           13110..13290
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="I9573_001146"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            13473..14690
                     /gene="cdeC"
                     /locus_tag="I9573_001147"
     CDS             13473..14690
                     /gene="cdeC"
                     /locus_tag="I9573_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HAT4795774.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDECNPCNPCNPCKPNPCNPCKPNPCDDKCECHDKCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSIPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(14791..16113)
                     /locus_tag="I9573_001148"
     CDS             complement(14791..16113)
                     /locus_tag="I9573_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAT4795775.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(16164..16958)
                     /locus_tag="I9573_001149"
     CDS             complement(16164..16958)
                     /locus_tag="I9573_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HAT4795776.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQYEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            17068..18513
                     /locus_tag="I9573_001150"
     CDS             17068..18513
                     /locus_tag="I9573_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAT4795777.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSSNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTNKYKLSKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISVISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            18542..18751
                     /locus_tag="I9573_001151"
     CDS             18542..18751
                     /locus_tag="I9573_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HAT4795778.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            18771..20165
                     /locus_tag="I9573_001152"
     CDS             18771..20165
                     /locus_tag="I9573_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAT4795779.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTALPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGFDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            20405..21157
                     /locus_tag="I9573_001153"
     CDS             20405..21157
                     /locus_tag="I9573_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4795780.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDYLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            21183..21596
                     /locus_tag="I9573_001154"
     CDS             21183..21596
                     /locus_tag="I9573_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HAT4795781.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDIECEYIILSDLYGASVCTAMLRLTNLHNVKLFSGMNLNMVLELLTR
                     FSDELTVEDIEQLVEESRRGIQSVTLQIKEEEEVF"
     gene            21600..22343
                     /locus_tag="I9573_001155"
     CDS             21600..22343
                     /locus_tag="I9573_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HAT4795782.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLELGDMAIIGSYF
                     KDNYKDFGNVSVEHVKTFMDEIKKIREEL"
     gene            22343..22813
                     /locus_tag="I9573_001156"
     CDS             22343..22813
                     /locus_tag="I9573_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4795783.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDELLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            22830..23588
                     /locus_tag="I9573_001157"
     CDS             22830..23588
                     /locus_tag="I9573_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT4795784.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            23572..24414
                     /locus_tag="I9573_001158"
     CDS             23572..24414
                     /locus_tag="I9573_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAT4795785.1"
                     /translation="MTTSSKKLDTISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSSSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGIV"
     gene            complement(24574..25479)
                     /locus_tag="I9573_001159"
     CDS             complement(24574..25479)
                     /locus_tag="I9573_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT4795786.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKGVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT         9987 a         2858 c         4646 g         8014 t
ORIGIN      
        1 atatagagcg tagtttgatt aaggttataa atttctcgac gtatattcaa ataccttatt
       61 atagatgttg ccgagatttt aattaataaa tgaaaataaa atatttataa aatactgagg
      121 aggctattat ggatttaaat tctaaaaaat atcagatgct taaagagcta tatgtaagct
      181 tcgctgaaaa tgaagttaaa cctttagcaa cagaacttga tgaagaagaa agatttcctt
      241 atgaaacagt ggaaaaaatg gcaaaagcag gaatgatggg tataccatat ccaaaagaat
      301 atggtggaga aggtggagac actgtaggat atataatggc agttgaagaa ttgtctagag
      361 tttgtggtac tacaggagtt atattatcag ctcatacatc tcttggctca tggcctatat
      421 atcaatatgg taatgaagaa caaaaacaaa aattcttaaa accactagca agtggagata
      481 aattaggagc atttggtctt actgagccta atgctggtac agatgcgtct ggccaacaaa
      541 caactgctgt tttagacggg gatgaataca tacttaatgg ctcaaaaata tttataacaa
      601 atgcaatagc tggtgacata tatgtagtaa tggcaatgac tgataaatct aaggggaaca
      661 aaggaatatc agcatttata gttgaaaaag gaactcctgg gtttagcttt ggagttaaag
      721 aaaagaaaat gggtataaga ggttcagcta ctagtgaatt aatatttgag gattgcagaa
      781 tacctaaaga aaatttactt ggaaaagaag gtcaaggatt taagatagca atgtctactc
      841 ttgatggtgg tagaattggt atagcagcac aagctttagg tttagcacaa ggtgctcttg
      901 atgaaactgt taaatatgta aaagaaagag tacaatttgg tagaccatta tcaaaattcc
      961 aaaataccca attccaatta gctgatatgg aagttaaagt acaagcggct agacaccttg
     1021 tatatcaagc agctataaat aaagacttag gaaaacctta tggagtagaa gcagcaatgg
     1081 caaaattatt tgcagctgaa acagctatgg aagttactac gaaagctgta caacttcatg
     1141 gaggatatgg atacactcgt gactatccag tagaaagaat gatgagagat gctaagataa
     1201 ctgaaatata tgaaggaact agtgaagttc aaagaatggt tatttcagga aaattattaa
     1261 aatagataaa tattaaaata tacacaatag gtcgatatta aaatagatat ggaggaagga
     1321 tttatgaata tagtcgtttg tataaaacaa gttccagata caacagaagt taaactagat
     1381 cctaatacag gtactttaat tagagatgga gtaccaagta taataaaccc tgatgataaa
     1441 gcaggtttag aagaagctat aaaattaaaa gaagaaatgg gtgctcatgt aactgttata
     1501 acaatgggac ctcctcaagc agatatggct ttaaaagaag ctttagcaat gggtgcagat
     1561 agaggtatat tattaacaga tagagcattt gcaggtgctg atacttgggc aacttcatca
     1621 gcattagcag gagcattaaa aaatatagat tttgatatta taatagctgg aagacaggcg
     1681 atagatggag atactgcaca agttggacct caaatagctg aacatttaaa tcttccatca
     1741 ataacatatg ctgaagaaat aaaaactgaa ggtgaatatg tattagtaaa aagacaattt
     1801 gaagattgtt gccatgactt aaaagttaaa atgccatgcc ttataacaac tcttaaagat
     1861 atgaacacac caagatacat gaaagttgga agaatatatg atgctttcga aaatgatgta
     1921 gtagaaacat ggactgtaaa agatatagaa gttgaccctt ctaatttagg tcttaaaggt
     1981 tctccaacta gtgtatttaa atcatttaca aaatcagtta aaccagctgg tacaatatac
     2041 aatgaagatg cgaaaacatc agctggaatt atcatagata aattaaaaga gaagtatatc
     2101 atataattta aggggggata aagaatgggt aacgttttag tagtaataga acaaagagaa
     2161 aatgtaattc aaactgtttc tttagaatta ctaggaaagg ctacagaaat agcaaaagat
     2221 tatgatacaa aagtttctgc attactttta ggtagtaagg tagaaggttt aatagataca
     2281 ttagcacact atggtgcaga tgaggtaata gtagtagatg atgaagcttt agcagtgtat
     2341 acaactgaac catatacaaa agcagcttat gaagcaataa aagcagctga ccctatagtt
     2401 gtattatttg gtgcaacttc aataggtaga gatttagcgc ctagagtttc tgctagaata
     2461 catacaggtc ttactgctga ctgtacaggt cttgcagtag ctgaagatac aaaattatta
     2521 ttaatgacaa gacctgcctt tggtggaaat ataatggcaa caatagtttg taaagatttc
     2581 agacctcaaa tgtctacagt tagaccaggg gttatgaaga aaaatgaacc tgatgaaact
     2641 aaagaagctg taattaaccg tttcaaggta gaatttaatg atgctgataa attagttcaa
     2701 gttgtacaag taataaaaga agctaaaaaa caagttaaaa tagaagatgc taagatatta
     2761 gtttctgctg gacgtggaat gggtggaaaa gaaaacttag acatacttta tgaattagct
     2821 gaaattatag gtggagaagt ttctggttct cgtgccacta tagatgcagg ttggttagat
     2881 aaagcaagac aagttggtca aactggtaaa actgtaagac cagaccttta tatagcatgt
     2941 ggtatatctg gagcaataca acatatagct ggtatggaag atgctgagtt tatagttgct
     3001 ataaataaaa atccagaagc tccaatattt aaatatgctg atgttggtat agttggagat
     3061 gttcataaag tgcttccaga acttatcagt cagttaagtg ttgcaaaaga aaaaggtgaa
     3121 gttttagcta actaatttct tggggagggt attatgagta caagtgatgt taaagtttat
     3181 gagaatgtag ctgttgaagt agatggaaat atatgtacag tgaaaatgaa tagacctaaa
     3241 gcccttaatg caataaattc aaagacttta gaagaacttt atgaagtatt tgtagatatt
     3301 aataatgatg aaactattga tgttgtaata ttgacagggg aaggaaaggc atttgtagct
     3361 ggagcagata ttgcatacat gaaagattta gatgctgtag ctgctaaaga ttttagtatc
     3421 ttaggagcaa aagcttttgg agaaatagaa aatagtaaaa aagtagtgat agctgctgta
     3481 aacggatttg ctttaggtgg aggatgtgaa cttgcaatgg catgtgatat aagaattgca
     3541 tctgctaaag ctaaatttgg tcagccagaa gtaactcttg gaataactcc aggatatgga
     3601 ggaactcaaa gacttacaag attggttgga atggcaaaag caaaagaatt aatctttaca
     3661 ggtcaagtta taaaagctga tgaagctgaa aaaatagggc tagtaaatag agtcgttgag
     3721 ccagacattt taatagaaga agttgagaaa ttagctaaga taatagctaa aaatgctcag
     3781 cttgcagtta gatactctaa agaagcaata caacttggtg ctcaaactga tataaatact
     3841 ggaatagata tagaatctaa tttatttggt ctttgttttt caactaaaga ccaaaaagaa
     3901 ggaatgtcag ctttcgttga aaagagagaa gctaacttta taaaagggta aaaatctaat
     3961 taaaaatata tttgctgtta actagattaa atagtatttt aatctagtta acagaatata
     4021 aatactaaaa taacaggagg ttataactat gaaattagct gtaataggta gtggaactat
     4081 gggaagtggt attgtacaaa cttttgcaag ttgtggacat gatgtatgtt taaagagtag
     4141 aactcaaggt gctatagata aatgtttagc tttattagat aaaaatttaa ctaagttagt
     4201 tactaaggga aaaatggatg aagctacaaa agcagaaata ttaagtcatg ttagttcaac
     4261 tactaattat gaagatttaa aagatatgga tttaataata gaagcatctg tagaagacat
     4321 gaatataaag aaagatgttt tcaagttact agatgaatta tgtaaagaag atactatctt
     4381 ggcaacaaat acttcatcat tatctataac agaaatagct tcttctacta agagaccaga
     4441 taaagttata ggaatgcatt tctttaatcc agttcctatg atgaaattag ttgaagttat
     4501 aagtggtcag ttaacatcaa aagttacttt tgatacagta tttgaattat ctaagagtat
     4561 caataaagta ccagtagatg tatctgaatc tcctggattt gtagtaaata gaatacttat
     4621 acctatgata aatgaagctg ttggtatata tgcagatggt gttgcaagta aagaagaaat
     4681 agatgaagct atgaaattag gagcaaacca tccaatggga ccactagcat taggtgattt
     4741 aatcggatta gatgttgttt tagctataat gaacgtttta tatactgaat ttggagatac
     4801 taaatataga cctcatccac ttttagctaa aatggttaga gctaatcaat taggaagaaa
     4861 aactaagata ggattctatg attataataa ataagaattt gggaattaaa agtttaaata
     4921 aaattgttta aaaaacaatt tcggatatat gaaaaatcta atttaatggg ggtaatgaat
     4981 atgagagaag tagtaattgc cagtgcagct agaacagcag taggaagttt tggaggagca
     5041 tttaaatcag tttcagcggt agagttaggg gtaacagcag ctaaagaagc tataaaaaga
     5101 gctaacataa ctccagatat gatagatgaa tctcttttag ggggagtact tacagcaggt
     5161 cttggacaaa atatagcaag acaaatagca ttaggagcag gaataccagt agaaaaacca
     5221 gctatgacta taaatatagt ttgtggttct ggattaagat ctgtttcaat ggcatctcaa
     5281 cttatagcat taggtgatgc tgatataatg ttagttggtg gagctgaaaa tatgagtatg
     5341 tctccttatt tagtaccaag tgcgagatat ggtgcaagaa tgggtgatgc tgcttttgtt
     5401 gattcaatga taaaagatgg attatcagac atatttaata actatcacat gggtattact
     5461 gctgaaaaca tagcagagca atggaatata actagagaag aacaagatga attagctctt
     5521 gcaagtcaaa ataaagctga aaaagctcaa gctgaaggaa aatttgatga agaaatagtt
     5581 cctgttgtta taaaaggaag aaaaggtgac actgtagtag ataaagatga atatattaag
     5641 cctggcacta caatggaaaa acttgctaag ttaagacctg catttaaaaa agatggaaca
     5701 gttactgctg gtaatgcatc aggaataaat gatggtgctg ctatgctagt agtaatggct
     5761 aaagaaaaag ctgaagaact aggaatagag cctcttgcaa ctatagtttc ttatggaaca
     5821 gctggtgttg accctaaaat aatgggatat ggaccagttc cagcaactaa aaaagcttta
     5881 gaagctgcta atatgactat tgaagatata gatttagttg aagctaatga ggcatttgct
     5941 gcccaatctg tagctgtaat aagagactta aatatagata tgaataaagt taatgttaat
     6001 ggtggagcaa tagctatagg acatccaata ggatgctcag gagcaagaat acttactaca
     6061 cttttatatg aaatgaagag aagagatgct aaaactggtc ttgctacact ttgtataggt
     6121 ggtggaatgg gaactacttt aatagttaag agatagtttt agattataat atttacaaat
     6181 taaatattta attatgaatt taacctgtat gaataactat ttgttattct tacaggtttt
     6241 tatagttaaa aataattatt atctattaaa caaaaatagt gtaaaatata taatatcatg
     6301 attttgaaga tataaaaagc aggaagatat aaaagaaagt aggtaaaagt ttgtttaaaa
     6361 aagagaatca aagatataat cttatatcgt atacaaatga agaagaaatg actttaaaag
     6421 aagttttatt ggacaagcta aatttctcag ttaggtcatt atcaaaaatg aagaaggaaa
     6481 agagtgtttt agtaaatgga gtatataaaa aaccaagttt aaaggtgtat agtggtgatt
     6541 taatagaagt aaaaatagac gaggaaaaag caaattttga acctcaagat ttaaatttac
     6601 agataattta tgatgatttt gatataatta tggtgaataa acctccattt atggtagttc
     6661 acccaacaaa aagccactat gataaaacaa tagcaaatgg tattagttac tatatagata
     6721 atcaaaaaga aaacgtaaaa ataagatttg taaataggtt agatatgaat acttcagggt
     6781 tagttatagt agcaaaaaat gcttatgcac atcatacttt atctacagca atgagtgaaa
     6841 ataaagttga aaaaaagtat ataacagtag ttgatggaat tataaaagaa aatgagggaa
     6901 ctatagatga gccaatttat agacctacag aagattcaat aaagaggata atagatgaaa
     6961 gaggacagtc atctgttact cactataaag taatagaaag actagaaaat gcaactgtat
     7021 tagaagtaag cttagaaact ggtagaacac atcaaataag agtacatatg gctcatatag
     7081 gtcatggaat aataggtgat gagttgtatg gttatgtaga tgaagagcta ataaataggc
     7141 aagcacttca tgcatacaag ctagagtttg aacaaccaag aacaaaagaa aaattgaaat
     7201 ttaaagcaga tataccagag gatatgaaag agttaatatc gaaattaaga taatggggtg
     7261 atatatatga taatacataa atttataata catgttttgg acaaaaatag tgatgttcca
     7321 atattaaatg actttgaagg caaagttaat caagaagtag atggtttttt ccagaaagct
     7381 ataaaaagaa ttgcaaaaga tgaggattta agaaaaggtg tttttaaaga ttataatgac
     7441 aatttaatta aaaactgttg tgaacaaata atatatgatg aaagtacttt tttgaaaaat
     7501 tctaaagaaa ttgcatctta cttgtttgac gttatgaaaa ttaatgctgc actagaatct
     7561 tgtgatttgg caatttgctt atacactatt aaagatgaaa agagtgtagc aatactgaaa
     7621 ttagattata aaaagttgta cactcattca attgaatttg tggatgataa atttaatatt
     7681 caaatggttt caaatgaaat tggtatccca gagactttaa gacaaaagca aggagctttg
     7741 attagtttaa gtggtataaa tgatgagttt catctaaggc ttttagataa ggatgctgaa
     7801 aaagaagggt cagaatctaa gtttgtaaca gaatttttaa atgctaaaaa aatagatgat
     7861 gataagtaca agaccaaggt atttaaaaat acagctgaaa attggataac taatgctctt
     7921 agtaatgata taaagcaagc tgaagatgta agaagtatat taaattatac actgaaggaa
     7981 aaacatgaag ttgatataaa tgactttgtt gataattcaa ttaaagatga tgagttaaag
     8041 gatagcttta aagagcatat ggaagaaaaa ggtcttgatg aaagttttag tatagataaa
     8101 aaatgggttg agaaaaagct taaaaaaaga agtataaaaa cggataatgg ttttgacata
     8161 aaagggaatc taactgattt tgaagaccca atgaagtata ctgtaaagca aaatcaagat
     8221 ggaactatag atataataat aaagaatgtt acattttatg aagaaaagta atttatatac
     8281 ttagtatata aattaaatat aagagcgtgt attttatata ttgtaaataa atgtaatcta
     8341 gtctgtgcga actctaatat aatttattat gtagaagttt acacagacta tttatatttg
     8401 tagacaaaac gatactattt gttttcttcg atatatttac atatatcacc aatacattta
     8461 aagttctcag cttcctcgtc tggtatttct attccaaatt catcttcaag ggccattata
     8521 atttcaacag catctaatga atcagcttct aagtcgtcca ttaaagatgc ttccatagtt
     8581 atctcatcta aattgtctac ccctaattgt tctgctatta tttcttttat tttctcaaac
     8641 attttaagcc tcctaaagca gttttaatta attttaatgt tagtttaata taatatcaag
     8701 aatttttcaa tatggaatct ataaaaaata ataaaatttt ataaatgtag taataaagta
     8761 ttatttgatg aataaacttt attgataaca aatataactt aggaggaaat gctatgaaat
     8821 atgtaggact attactaact tcagtaggaa tgtttttact tatagcagta aatttttatt
     8881 ataattcaat aactttagat atgcaacgta tagaggatta tgtaatggag accaatttaa
     8941 tccttgaaga tgtagctgaa aaggagagtt atgtatctaa tgaaaaagaa gactacatat
     9001 caaggttaat gcatgtaaag aaaggaatag aaaattcaaa aacatccttc ttgatagaaa
     9061 gatataagga gtacaaaata aaatcaatag aaagtttaat ctacactata tcagaggaga
     9121 aaaaagatta tctggatgaa gttgatagat acaataaact tggtgaaaaa gaaataaata
     9181 aactaatcaa taaaaacttt ttagaagtaa cttatctgtc tattactaca tatatttaat
     9241 taaagagaca aaacttagtc tcttaatgat tcaaggagga ttagttatga caaaattaga
     9301 agacaatatg gaacgtttct ttggaggatg cgaaggattt tttggtaata aaatgttgat
     9361 aataatagtt atagtattct tactattatg cacagacatt ttagaagact tattatgcga
     9421 tgacaatata tggatatggg ttatattaat agtattgtta ttatttaact tcgatgatgg
     9481 ttgttgttgc taaaaaatat tgattattaa ataggtaaaa ccagtaactt atacaagtta
     9541 tggttttacc ttttttagtt caaattaaaa aatttaaaaa aatagtaaaa atacattagc
     9601 aaaatggaaa ccaaatgcat agtatatgtt aggagcgaaa agctcaacaa aatatcaaca
     9661 ggaggtaacg tacaatgtta gatagattct ttggagaaga aggatgtttt ggtggaggag
     9721 gatggtggat aatagtacta ttcttcctat tcttagcttt tggagatagc tgggcagaat
     9781 ttgacataat ggcatggata ccattcctaa tagttttatt aatactttgc tgctgtggtg
     9841 gatgcttaga ataagaacac agttattaaa aaaaggaggg ctttgttatg ttagatagat
     9901 tttttggaga agaaggatgc tttggtggag gaggatggtg gataatagta ctattcttcc
     9961 tattcttagc ttttgaagat tgctggagag acattgacat aatgtcatgg atacctttct
    10021 taatattatt gctaataact tgttcatgtg gtggattctt tgatggtgaa gtaggttgcg
    10081 gctgctaata cttaaatcat tagaataaaa aaatgctggt attttatacc agtatttttt
    10141 tattttattt tttaatatga tttaaattaa gaatgaatat tattaaattt gttgaatata
    10201 gggtataata taaaatataa tgtatttaaa tgtacttgct tttatggtaa aataaaaact
    10261 tatataaatg tagttaaaat aggaaccttc aaaaatttgg ttcagtttta gataaattta
    10321 gtaaaataag gaggatgaag atgaaaggca atataacgat aaaagatgtt gctaaacaag
    10381 caggagtgtc aatatctact gtatctagag ttataaatga ttcaaaacct gtaactgatg
    10441 aagtcaaaca aaaagtttta gaggttataa aagagactgg atatatacca aatccacttg
    10501 ctagaagctt agtaacaaag aagagtcaat taataggggt aatagttcca gaagtttcag
    10561 attcttttgt taatgaggtg ttaaatggga tagaagaagt tgctaaaatg tatgactatg
    10621 atattctttt agcgaataca tactctgata aggaacaaga acttaagagt ataaatctat
    10681 tgagagcaaa acaagtggaa ggtatagtta tgatttcatg gatagttgaa caagaacata
    10741 tcaactatat acaaaattgt ggaataccag cgacatatat aagtaaaact gctagaaatt
    10801 atgatatata tacagtaagt actagcaacg aagaagctac ttttgatatg acagagcatc
    10861 ttataaagaa aggtcatgaa aagatagctt ttataatgac gagtaaagat gatactgttt
    10921 tagaaatgga aagacttgct ggttatgaga aagcactttc aaataacaat atagaattag
    10981 acaagagttt gattaagtat ggtggaactg attatgagag tggatacaat agtatgaaag
    11041 aactattaga tgatggaata atacctcatg cggcttttgt aacaggtgat gaggctgcca
    11101 taggtgctat aaatgctata tgtgatgctg gatataaggt tccagaagac atatctgttg
    11161 caggatttaa tgatgttaag atagctagaa tgtatagacc taaacttact acagtatatc
    11221 aacctctata cgatatggga gcagtagcaa taagaatggt tataaaatta ataaataagg
    11281 aattaattga aaataagaaa atagaattac cttatagaat tgttgataga gaaagtgtta
    11341 cagaaagaaa aaaataaaac tattaaaatc aatcaggtag tgcctaaggc actacctgat
    11401 tttttatgtc aataatatca aatttaataa tagattaact aaaaataatt atctaattac
    11461 ttaaatagtt tatttttatc tactcatgag ggttagactt tttagttttc ttaaataaac
    11521 ctttagattt tttttgttga ccacctggtg tattttttgg tgcaggtgaa tttgatttac
    11581 ctttagtagg tttagccttc ttagttttag ctggtttagt atctctttga ttttctgcat
    11641 ctgagatttt ttccattata ttatctagtt ttttgcgttg attttcatct aaaaatgctt
    11701 tgaattttga tactacttca tctcttccat ctaagttctt acctattttt ataagttcct
    11761 ccataagttc attttctgat tttcctttgt aactatcagc catcttttct attttatcct
    11821 tgtcaatatt tttttgttta gccatttttt ctaatgactc tgtatctaat tttttcataa
    11881 aaaaacccct ttctataaat atatattgaa ttatattcat actatttata tatagaaata
    11941 atattgaagt ggtgaattgt gagtgatata taatataaat aaaaattctt atagataata
    12001 tatatgcact gagctttaaa aatggaacat taaaatatga aaaacatact ttaatatagg
    12061 gcaattatat aatataaggg ggaattgtct tgaataactt actatttttg attggagtga
    12121 ttgcattgag taatggtaat atctctatat ttgatgataa aaataaatca aaaggaaata
    12181 atagagttag aaaaaacaaa aaaaatgcaa atgataaact ttcacagtct acacatggca
    12241 atttaaggtt tgatgcaaat gatataaaaa gaggtttaag gctcatggat ttaagtgaag
    12301 aagatttaga aatgggaatg gaaataataa caagaactaa aaaatacatg tcaagagatg
    12361 aaagaaaaat actaataaaa atagaaagtg ttttagattt agttagagga ataaaaaaat
    12421 taaataatat agatatggta gatacagaag atgaaacgga ttttttcaga aaaatggata
    12481 gtgatgataa aaagaatatg atgataaagg aaattataga tgtattccca gaaaaaagaa
    12541 aaaattcagt tgaaaaagct ataggaatga aaaagaagat agatttattt gcagaattat
    12601 ttttaccaga tgactttggt gaaggtggat ttagtttgag ctctttggca aatataaaca
    12661 atcttggtag tatgaataat ctcaaattgt taggaagtct tttaagaggt gatgatagta
    12721 ataatgatga tgaagacaat aatgatgagt taaattatga agacgaagaa ttagattgtg
    12781 aagacaaaga attggatgat gatgagttaa cagatgaagg tgaaatggag gataatgaag
    12841 atgaattagt agaagataag aacacaggct atgaaaatat agaaaatgat gaagatatag
    12901 tatacgatga ggaactatac aacattaatg agcaagaatc cgaatacaat tataatgaaa
    12961 aaaatactaa aactagagct aagaaaaaac gataattaac taaatacata aagaaaaaag
    13021 gaagaaaaag taatgataga ataagattat ctcaattact atataaaacc actttaaaaa
    13081 attcaaaatt tattatataa taataatatg aaatcctgaa acatacgata aggctttttt
    13141 aaattcaacc tacaaatgaa atacgggaga ccgtgtctgg taaattataa catgcctagt
    13201 tagtttggac gttaaaggtt actggcaagt cacccacctg ggagtgccta gggtgtgaat
    13261 ttacttagcc accggtgttt cgggacaaaa aaagtgctac tatttcaaat agtagtactt
    13321 tttttattaa tatgcatttt tctactaaat acgcctaatt attgataaat tcaaaaagca
    13381 agtaacaatt aagattataa aagcatagta ttaattaggc ttcaaaaagc caaagactta
    13441 ataagaatcg cttacaggga gggcaatgaa aaatgcaaga ttataaaaaa aataaaagaa
    13501 gaatgatgaa tcagccaatg tctacaatga atgaagaaga agtgtataca gatgaaataa
    13561 attcagaaga catgagaggg tttaaaaaat cacaccatca taatggatgt aatactgata
    13621 ataagtgtga gtgccatgat gagtgtaatc catgtaaccc atgtaatcca tgtaaaccta
    13681 acccatgcaa tccatgcaaa cctaatccat gtgatgacaa atgtgaatgc catgacaagt
    13741 gtaaatgtga ttgtgaacca tgtgaaatgg attcagatga atgttttgaa aacaaatgtg
    13801 gaccagaatg ttgtaatccg atatctccaa gaaacttctc tgtatcaaat gcagtgccat
    13861 ttgcaataga ggctaataga atatttgata ctatgcaatt ccaaacattt acagatgcaa
    13921 caggtccaaa tggagaacca ttaacttttg aaacagaagt agtagaagta tttggttcaa
    13981 ttccaagtgc aggtcaagca agtgtaacta tagaaaaaat atgcttaagt aatgatggaa
    14041 tagttataga cacaggaatg acaactttag aagatttcga tttagaccca ttaggagata
    14101 tagtaggaag aaactgtgaa acaacttttg aatttgcagt ttgtggagaa agaaacgctg
    14161 agtgctgtag acaaggaaaa ggcaaatcag tagcttataa acaaagagga ttaactgtag
    14221 cagttcgtaa tttagtacta gagctaagag gtagatgtgg atgtacagag ttcgttgcat
    14281 tagctttccc agcagttaga gcaggaggtg gatgtaagag aagagttgat tatgtagaat
    14341 ttacttttaa cacactttca gcaccaatat gcttgccagc tgacggaaga gctgttactt
    14401 taagacaaga atatcaaact aacttaactg tagattgtat aggaaaatct atattaaaat
    14461 tagaatgcaa cgaatgttgt gaacctttct atgaattaat tataccaaat gatatagatt
    14521 tagtactttg cttacaagaa acagttagca cattaataag tgaacaaata gtagttttag
    14581 catcaccaaa tccaatccaa ccaagacttg ttgatacttt ctctaaagta tgtgattttt
    14641 cgcaatgtgg acctaatcat ggaagtggaa agccaagttg ccacagatag ataggtaaga
    14701 tatatagaca gtttaaatta taaataaaaa tcatctaagg aaggctacca aagtgtataa
    14761 tttacattgg tagccttcac gtttttataa ctagcgtata gattttagtg atttaaaaag
    14821 cactatagta tgtaatagac aagtaaagat tgatactatc gtcattgtgt atatgtatgc
    14881 ataaatatta agtcctggaa ttggaagaaa tacatataaa gctattagtt gaattaacat
    14941 gccagtaata gtatttatac ttgaagctaa ttcttttctt attgaatgta aaatgccaga
    15001 tagagtttga tttagggaca taaatagtgg aactaaaaac atagctttta gatattctcc
    15061 tgcaagtgta tttttaaata ctaatataca taaatctttt ccaaagaaat aaaaaaatat
    15121 tgatgatagt atgcctacaa ataaagtaag aagtatggag tatttgattt ttttaattac
    15181 actcttacgc tttcttagag ccatttcctg tgaaatagtt ggtattaaat ttacaataag
    15241 cgctgaacca acagtaaaag gtaaaaatat aaagggcatt accatcccac tgacaatacc
    15301 atacatactt aaagattgtt gataagtcat tcctgataat gctaatcttg atggtatcat
    15361 catagaactt atagattgca aaagtatgtt tgacattcta ttacaagtta tgggaataga
    15421 catttttaat gtttccatag aagaattata aaaatctttt atgcttataa tatacctatt
    15481 gtcaaatgat gaatctcgcc ataagcaaat aagcataaat ataatattgc tcaactcacc
    15541 aatagatata cctaatagag ctatataaca attcatagat ttattattta tgtacataac
    15601 aagtaaaaaa acaaataata ttttaccaat ttgttcaatg atttgtccaa tagcagggat
    15661 ttttacattc tttatgccat agtaatatcc tcttagtacg tttgaaagag ttattataac
    15721 tatagcagga caaacagcta gtataaatag atttaattta gcatctttta aaaatttaag
    15781 tgataaaaaa gaactattga aagatgcaac tatagatata attagtgcta cgaaaaatga
    15841 tatatataaa gttgatataa atagagcatt agtattgtgt ctatcgttta aagcttttct
    15901 ctttgcaact agacaactaa gtgcagtagg tatacctgtt gttgtaacag ctaagcaaat
    15961 catcaaaaag ttaaaaatca tatgatagat acctaaacct gtttcaccta gtgctcttga
    16021 taagaatatt ttatataaaa aaccaaatat tcttacaata aaattggata taaataaaat
    16081 aactgttgat agtactaaat aaggtacttt caaaaaaatc acctccttat cttttaaata
    16141 tattcacaag gaggtgtaaa aatttataat tttataaatt ctggtttcaa tttattaaat
    16201 ttagatacat atttaaatcc catatttttt agtaatgaat atatgtagtc aaactcacat
    16261 gcaacttgac ttatatgatg tgaatctgaa cctgtggtta tgatttcccc tcccaagtct
    16321 ttgtacattt gaagtatata cttagatggg gttaggtctg gaagattata tctataacaa
    16381 gaggtattta tctcgatacc tttgccatca taaatagctt gtttaagagt ttcttcaatt
    16441 atatcagaaa atagtctatc atctaaaata gtgtcataag gtgcatacct tttaatcaaa
    16501 tctaaatgac ctaagacaga gtaatcttta taattcttaa caatattata taaataaaga
    16561 tagtaatttt cataaacttc atattgagtt ttatctttaa aataattacc aaggtataag
    16621 tccattgtat ctatagcatg gatagaacat attataaagt ccaatggata ttgatgtgct
    16681 tcctttttgt atgtatccat taattgagtt tgaactccaa attcaattcc tttttttatt
    16741 gatattttat ctttgtattt gctttgaaga gtctctaaac tcttaaaata atcttcgtag
    16801 acaattgaaa aagaatcatc agcatataca tcatagtcaa catggtctgt gaaacaaatt
    16861 tcctccaaac cgagttcaat agattttttt accatttctt ccattgtaga ttttccgtct
    16921 gtagagaaac ttgagtgcat gtgatagtcg taaaacataa atatttcccc tttttataaa
    16981 aattttggta ttatatatac atactaaata atatcaaata tatataaaag catcaataaa
    17041 atataaatac aatgagcggt gataaatttg gaaaaataca gaatagattt aaatgagaaa
    17101 aaatcaacta aatatataca gatatttaat catataaaag aattgataat aaataaagag
    17161 ttagtagaac atgaaaaatt gcctccaata agaaaactat caaatttttt acaagtaaat
    17221 aacactacta tagttaaggt atacgagctt ttagaaaatg aaggatatgt atataaaatt
    17281 atagggagtg gtacttttgt atcaagttca aattcttata aaagcggtat agataacagg
    17341 aaaaataaga ttattgtgca aagagaagat ttaattcatt ttgacaatgg gaatccatct
    17401 aatgatatgt ttcctatcga ttcatttaaa aatgctgtaa atatggcatt atctaaagat
    17461 ggtagttcaa tatttgaata tgatgaaggt cttggctctg aagaattaag agaaaaaatg
    17521 gtagagtatc taagtgatgg aaatataaaa actactaagg aaaatattca aataatatca
    17581 ggtgctcaac aaggtataga tatagtttgt aaaggactta taagttattc agatgttgtt
    17641 tttatggaag aaccttctta caatggagca attgaagtgt ttaaaagtag aggtgcaaaa
    17701 ataatatcta tacctatgtt agatgatgga attgatatag gtattttaaa attaaagttg
    17761 gaaaaaataa aaccaagact tatatacatt atgcctaatt ttcaaaatcc aactggaata
    17821 tcttactcaa catataaaaa aaagaaattg atagaattgg cagaagaaca tgatttttat
    17881 ataatagaag atgattttat aagtgatttt gtttttgaat caaaggacaa tagaacaatt
    17941 agaagttatg atgataaaaa tagagtagta tatataaaaa gcttttcaaa aatacttatg
    18001 ccaggtctta ggataggaat tgtagaaatg ccaaatgagt tgttaaaaaa agttttatgg
    18061 gctaaatatt catcagatat atcaacacca ggtttaattc aaaaatcaat gtattactat
    18121 atgaataatt ttgattggaa gaattatttg agtcatatag agaagatata tactaataag
    18181 tataaattgt caaaaaactt gattaatgac aaattgagtg gtaaattaaa agttagaaaa
    18241 tcttgtggag gaataaactt ttttttagaa ttaccaagag gatatacatc tcaagcattt
    18301 acaaatttta tgttaaacaa aggagtgtca atgcttccag gaacttattt ttttgacaat
    18361 ctagtggatg atagattctt taggataaat atagctcgcc caagcatgga agaactagaa
    18421 aagggaatat ctgtaataag tgataatata gatgagtttt tttgtgaata taaaaacaag
    18481 ttagatataa aaagcaataa attattttat taaataaata gaggtttttt aggaggaaaa
    18541 aatgtttgac aagaagaaat tagataggat taatgaatta gcaaagaaaa ataaagaggg
    18601 aatcctttct gctgatgaaa tcaaagaaag agaaatttta aggaaagaat atttagaaaa
    18661 ctttagagct cactttagat caagactaga tagtgtcaaa gtagtttcac cagaagaata
    18721 tgagcaatac atgaaaaaca ataaaaatta gaacttagta ggaggaaaat atgaacttaa
    18781 agtacgaatt taagaatgct tgggattttg aaagaaaaga aaatactatt gaacaaatta
    18841 tgcaatattc tcgcaattat atggagtttt taagtaagtc aaagactgaa agactttcag
    18901 taaaagaaat tataaaattg gctaaagaaa ataactatat ttcaatagat gaagctatgg
    18961 aaaaaggaag cataagttgt ggtgataaga tttatgttat aaataaagag aaggcagtag
    19021 cactttttgt aataggtaaa aattatatag aaaaaggtat gaagattata ggaagccata
    19081 tagattcacc aagattagat ttaaaaccaa atccactgta tcaagagagt aatctaggtt
    19141 tttttaagac tcattattat ggtggaataa aaaaatacca gtggacagct cttccacttg
    19201 ctttacatgg tatagttata ttaaatgatg gaacaaaagt ggacatatct attggggaag
    19261 aagatagtga cccagtattt tgtgtaactg atttattgat tcatttggct ggagaccaaa
    19321 tgcaaaagaa actatcagaa ggaattagtg gagaagcttt aaatatatta attgggaata
    19381 ttcctttaga agatgaagaa aaagaaccta taacagcaaa tatattaaag attttaaatg
    19441 agaaatacaa tatagttgaa gaagacttat taagtgctga aattgaagta gttccagctg
    19501 gtaaggctag agatttaggg tttgataggt ctatggtgtt gggatatggg catgatgata
    19561 gggtatgttc atatgctgct gtaaaagcta tactagaaac tgaacaacca gaatttactt
    19621 cagttgcatt atgtgtagat aaagaagaaa ttggttctaa agggaataca ggtatgcact
    19681 caaagttttt tgaaaatact gtggctgaac tgattgcttt agaaggagat tattgtgata
    19741 ttaaagtaag aagagcttta gcaaatagta aggtattatc agctgatgtt tctgctggat
    19801 atgacccaaa ttttggagaa gcatatgaaa agagaaattc agcatatatg ggaaatggag
    19861 tagttttaac taagtataca ggttcaagag gaaagagtgg atgtaatgat gctaatgctg
    19921 aatttatgtc agaagtaaag agaattttta ataaaggaaa tgtagtatgg caaactgctg
    19981 aacttggaaa agtagaccaa ggaggaggcg gaactatagc ccatatattg gcaaatcaag
    20041 gtgcagaggt tatagactgt ggtgttggag ttttaaatat gcatgcacca catgagattg
    20101 tctctaaagt agacatatat gaaatgtata agggatataa ggcatttttt aatataaatt
    20161 tataagtaaa aatataaatg taataagtga ataaaggctg ttgtataaac aatttaatct
    20221 gttgtacaac agtcttattt ttatgtaaaa atagcaattt gtcacactag agaaaataaa
    20281 agaaaatact gtcaaattaa aaattattta aaaaaaaatt gacaacgatt tcatcttgtt
    20341 ataatatgta ataaagaact tgttatataa tgttataaaa atgttacata gggaggtgag
    20401 caaaatggat gaaaagagac aaaaaaaaga aatggattta gaagtaaatt cctttgttcc
    20461 tttatatcaa caactatatg acaacataaa aaaacaaata gcatctggca tatataaacc
    20521 aggagataaa cttccatctg aaggagactt atgtaaagaa tttaatataa gtcgtataac
    20581 tgtgagaaat gcacttaatg aacttgtaaa agaagatata ttatgtaaaa aacgtggtaa
    20641 agggacttat gtaacaatac cagagagaat agaagcaaca tgtgcaggaa atagttttac
    20701 taattcttgt catcgtatta atgcaaaacc aagtaccaaa ataatctctg ttttaataaa
    20761 aaaagcagat aagcaagtag ctgaagcttt aagtattgaa ctagaagaga aggtaatatg
    20821 tattaaaaga cttagattaa tagatgaagt acctgttatt tttgaagttg actattttag
    20881 aatagattac atgtttttat taaaagaaga attagaagat aaatctttaa tggaggttat
    20941 atctaataat ataagcacat tacctaaacg agtagaaaat atatttgaag taaaacattc
    21001 aaacaaagaa tattctgact atcttaaatg tgcaagtaat atgccactat tgaaagtaag
    21061 acaatcggtg tatacagaaa ataatgatgt cttatattac aatgagcaat ttataagaag
    21121 tgataagtac aaatatgcag tatcagcaga aatataatct atatttagta ggaggatgaa
    21181 gtatgaaaaa gataatatta gcatctcatg gagatttttc aaaagggctt ttaaatgcag
    21241 taaaaatgat agtaggagat ttagcagatt gtgttagttc gtatggtttg taccctggac
    21301 aaagtgcttc agattttgct ttagaattag aaaaaacaat atcagaagat atagaatgtg
    21361 aatatataat tttatctgac ttatatggag caagtgtatg tacagcaatg ttaagattaa
    21421 ctaatctaca taatgttaag ttattttctg gaatgaattt gaatatggta ttagaacttc
    21481 ttacaaggtt ttctgatgaa ttaacagttg aagatataga acaattagtg gaagaatcta
    21541 gaagaggaat acaaagcgtg acattacaaa ttaaagaaga agaagaggta ttttagttta
    21601 tgaaaaaaaa gggaatatta tcagtattta aaagtaaaaa accaattata gcaatgatac
    21661 acttaaaagg agatactcca gaagatatct ttgaaagagc taaaaaagag attacaatct
    21721 ttgaagaaaa tggtgtagat ggaataatgt tagaaaatta ctatggtaat tattatgatt
    21781 tagaaagaat acttgaatat gtatcaaaag ctaatttgtc aataccatat ggagtaaatt
    21841 gtttaaatgt tgatactatg ggatttgaac tggcaacaaa atataatgct agttatatac
    21901 aggttgattc tgtagtaggt catgttaaac ctagagacga agctacttta gaagaatttt
    21961 ttaaattaca acgttcaaaa tgcccagcgt atttaattgg aggcgtacgt tttaaatatc
    22021 agccagtatt atcagaaaat gacgttgaag aagatttaaa aataggaatg acaagatgtg
    22081 atgctatagc agttacagaa aatgctacag gtcaagaaac atcaatggaa aaaatagaac
    22141 tgtttcgtaa aaatttagga gactttccat tggttatagc tgctggagtt acattagaaa
    22201 atgcaaaaaa acagcttgaa ttaggagata tggcaataat aggaagttac tttaaagata
    22261 attacaaaga ttttgggaat gttagtgtag aacacgtaaa aacattcatg gatgaaatca
    22321 aaaaaattag ggaggaatta taatgattaa attagtaaga gtagaccaca gacttataca
    22381 tggacaagta gcatttacat ggacaaagtt tttaagcaca gactgtatat tgatagcaag
    22441 tgatgaatta ttaaaagatg aattaagaat ggcagggctt aaaatggcta aaccatctaa
    22501 tgttaagtta gtaatgaaaa gcatagcaga ttctataaaa gcacttaact caggtgttac
    22561 tgataaatat aacttattga tactttgtga atctgtagaa gatgtttata gacttgctaa
    22621 agaggtaaaa gctattaaat ctataaacct tggtggaaca aaatcagatg ataatcgtga
    22681 aaatatatct aaagcagtgc atgtatcaaa agatgatata aagatgatta aagagttaga
    22741 ttcagaaggc gtaaatgtat ttgtacaatt agttcctgat gatgatgcta caaatgtaat
    22801 gaaattaata taattttagg gggatgaaaa tggaatttac acaagttatt ttgataactc
    22861 taattgcatt ctttgcatat atgcatagtt ttgtaggttc tacaatgcat aatagaccta
    22921 tagttgttgc accacttgtt ggtttagcac taggtaatct tcatacaggt atagtaatag
    22981 gttcaacatt agaattagta tttatgggtg catttcctgt tggggcaagt aatcctcctg
    23041 attttgtgtc tggttctatt attgccacag cgtttgtaat attaacgggt caagatgtat
    23101 cagcagcagt tgtacttgca gttcctatag caactctagt attgcttata gataacttct
    23161 taatgacagt agtacttaca tggggagctc atattgctga ccgttatgca gaagagggga
    23221 atattgaagg cgtagaacgt gtgcaattat tatttggtat aggaaataaa ttaatattag
    23281 caataatagt aggtatagga ttttcattag gtgttccagt aatagaaaaa atactttcat
    23341 ttataccatc atatgtaact catggaatgg atgttgcagc aggcgtgatt ccagcaatag
    23401 gttttgccat gttagctagg atgatgctta ataagaaaac agtagcattt ttattattag
    23461 gatttatatt ggttgcttac ctaaacataa cagtaacagg agttgcattg tttggattag
    23521 caatagcttt aatatatgtt aactttgtag gagagaagga ggtaatagta gatgacaacg
    23581 agttctaaga agttagatac aatttcaccc gatagtaaaa taacgaggaa agacttttgg
    23641 aaatgcttta gaaggtcatt gacattagat tcatcttgga actacgaacg tatgcaaaat
    23701 atagcatatg catacatgat ggcaccaata atccgtagat tgtataaaga tgataaggaa
    23761 aagaaatcta aggctttaaa aagacattta gaatttatgt cagttactcc tcatataagt
    23821 actcttttgg taggtatatc tggagctatg gaagaagaaa atgcaaagaa taaagaattt
    23881 gatgctaata gtataaatgc tgttaaatca agtttaatgg gtccagtttc tgggatagga
    23941 gattcatttt tctggggaac attaaagctt atagcagctg gagttggtat agctttagct
    24001 tctcagggta acataatggg accgatttta ttccttttaa taataaatgt tcctcatttt
    24061 attatacgct atatatgtct agataaagga tttaagtatg gaactcaatt ctttaaagat
    24121 gtgagtagtt caagtatagt ttcaaaagtt atggaagcag cttcaatgtt agggcttatg
    24181 gtaattggag gtatgactgc atctaatgta atgttaaaac ttagtgttaa tgtaggtagt
    24241 ggagaatggg cagagcctat tcaaacttac ttagaccaaa taatgccatg tatgcttcca
    24301 gctatgatat tcggtattat gtattggtta ttaggaaaaa aggtaaagac aactacgata
    24361 ttaatttcag ttatgattat ttgtatagta ttagctgcta ttggaatagt ataaataaaa
    24421 aaatcccaag ttcatttgaa cgagggattt tttgactaaa ttaaataaaa aagaaagatg
    24481 gttcataata atttataagc atttatgtga tagttataat tagcttatta attagttata
    24541 aacccaaaat taagcactac tttattaaag ttattatatt ataatttcga aatactcaca
    24601 taatctgttt agaaatttat cttctaatgg ggaaaaagtt ttctttttag agtaaataaa
    24661 gaaaaatttt ctagtaaaat caacaccctt tattttataa cattttattt tacctgcatt
    24721 gagataatca atagctgaaa tgtacgatat aaaggagact ccaagaccta atctaaccat
    24781 ttccttaata gcctcatttg attctacatg agctattaca tttaatttac ttactggaaa
    24841 gttattttta ctaagtgtgt ttagtattaa gtttcttgta cctgaccctt cttttctcat
    24901 aataaaattt agataagcca attctcctat atcgatacaa ttatttttat tatcaatttt
    24961 aaaatctgaa ggagtgataa gaacaagttc atcatctaat aagtctaggt actctatttg
    25021 aggattattg attttagaac caactaatcc aaagcttatt cgttcattta aaatttctga
    25081 tatagcatat tgagaatcat aatggcttat ggaaaatttt acatctggat aactcataga
    25141 aaaactcttc ataaaatcag gtaaaatata agtttcaggt atggaactac atgctatatc
    25201 tataatacct tctatttttc ctgaatattc ttttatatca taaattgcac gtttgcaatt
    25261 attcaatata tatatagcat gttcataaag aatttcacca gattttgtga gtgtaataac
    25321 tttgttgctt ctatttataa gaacagtttc caactctctc tctaaattct gtatgtgtgc
    25381 acttacagta ggttgagtta aaaaaagttc tcttgcggcc tttgaaaaac tctgatgttt
    25441 tgcaacagca acaaatactt caagttgttt aaaatccatt atttaatcct ccttaaatta
    25501 tctaa
//
LOCUS       DACTGW010000036        25494 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.036, whole genome shotgun sequence.
ACCESSION   DACTGW010000036 DACTGW010000000
VERSION     DACTGW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.036"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(37..237)
                     /locus_tag="I9573_001160"
     CDS             complement(37..237)
                     /locus_tag="I9573_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795787.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(239..1171)
                     /locus_tag="I9573_001161"
     CDS             complement(239..1171)
                     /locus_tag="I9573_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore envelope protein"
                     /protein_id="HAT4795788.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNM
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(1216..2148)
                     /locus_tag="I9573_001162"
     CDS             complement(1216..2148)
                     /locus_tag="I9573_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795789.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYDLSFFHCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNDVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(2203..3492)
                     /locus_tag="I9573_001163"
     CDS             complement(2203..3492)
                     /locus_tag="I9573_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAT4795790.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(3521..4213)
                     /locus_tag="I9573_001164"
     CDS             complement(3521..4213)
                     /locus_tag="I9573_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4795791.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(4228..4986)
                     /gene="pgeF"
                     /locus_tag="I9573_001165"
     CDS             complement(4228..4986)
                     /gene="pgeF"
                     /locus_tag="I9573_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HAT4795792.1"
                     /translation="MKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNIDA
                     KNREDMLKVCKECDLTFENITSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPLL
                     LFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIGP
                     SIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKENI
                     INLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(4986..5447)
                     /gene="nrdR"
                     /locus_tag="I9573_001166"
     CDS             complement(4986..5447)
                     /gene="nrdR"
                     /locus_tag="I9573_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAT4795793.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIEEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(5469..5732)
                     /locus_tag="I9573_001167"
     CDS             complement(5469..5732)
                     /locus_tag="I9573_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HAT4795794.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(5836..6609)
                     /gene="sigG"
                     /locus_tag="I9573_001168"
     CDS             complement(5836..6609)
                     /gene="sigG"
                     /locus_tag="I9573_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HAT4795795.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(6701..7423)
                     /gene="sigE"
                     /locus_tag="I9573_001169"
     CDS             complement(6701..7423)
                     /gene="sigE"
                     /locus_tag="I9573_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HAT4795796.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(7448..8320)
                     /locus_tag="I9573_001170"
     CDS             complement(7448..8320)
                     /locus_tag="I9573_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HAT4795797.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGSAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(8611..9885)
                     /locus_tag="I9573_001171"
     CDS             complement(8611..9885)
                     /locus_tag="I9573_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAT4795798.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(10169..11326)
                     /gene="ftsZ"
                     /locus_tag="I9573_001172"
     CDS             complement(10169..11326)
                     /gene="ftsZ"
                     /locus_tag="I9573_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAT4795799.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(11516..11869)
                     /locus_tag="I9573_001173"
     CDS             complement(11516..11869)
                     /locus_tag="I9573_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HAT4795800.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(11968..12642)
                     /locus_tag="I9573_001174"
     CDS             complement(11968..12642)
                     /locus_tag="I9573_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HAT4795801.1"
                     /translation="MKKKIVILGAFILGIFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(12647..13345)
                     /locus_tag="I9573_001175"
     CDS             complement(12647..13345)
                     /locus_tag="I9573_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HAT4795802.1"
                     /translation="MKKRFINKSIIIGSVVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKTEKENLQSEVNSVKKDIETYRGKNNKGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVFNTYMY
                     LKEDKLCMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(13368..14108)
                     /locus_tag="I9573_001176"
     CDS             complement(13368..14108)
                     /locus_tag="I9573_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAT4795803.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNINLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSVDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(14378..15604)
                     /gene="murG"
                     /locus_tag="I9573_001177"
     CDS             complement(14378..15604)
                     /gene="murG"
                     /locus_tag="I9573_001177"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAT4795804.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(15622..16752)
                     /gene="spoVE"
                     /locus_tag="I9573_001178"
     CDS             complement(15622..16752)
                     /gene="spoVE"
                     /locus_tag="I9573_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HAT4795805.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(16804..18159)
                     /locus_tag="I9573_001179"
     CDS             complement(16804..18159)
                     /locus_tag="I9573_001179"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAT4795806.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEVVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(18178..19146)
                     /locus_tag="I9573_001180"
     CDS             complement(18178..19146)
                     /locus_tag="I9573_001180"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAT4795807.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(19187..20560)
                     /locus_tag="I9573_001181"
     CDS             complement(19187..20560)
                     /locus_tag="I9573_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAT4795808.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREASAQNAFVA
                     IVGENLDGHTFMKPAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDIYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(20727..22706)
                     /locus_tag="I9573_001182"
     CDS             complement(20727..22706)
                     /locus_tag="I9573_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HAT4795809.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(22718..23065)
                     /locus_tag="I9573_001183"
     CDS             complement(22718..23065)
                     /locus_tag="I9573_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795810.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIAIFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(23551..24486)
                     /gene="rsmH"
                     /locus_tag="I9573_001184"
     CDS             complement(23551..24486)
                     /gene="rsmH"
                     /locus_tag="I9573_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAT4795811.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGKLVDIIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNKGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(24516..25262)
                     /locus_tag="I9573_001185"
     CDS             complement(24516..25262)
                     /locus_tag="I9573_001185"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAT4795812.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKDDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKHNISEE"
BASE COUNT         7753 a         4787 c         2596 g        10358 t
ORIGIN      
        1 ttgaatatta tttgttctta aaaaaatttt ataaatttaa tgttttacta tgattaacgt
       61 cccatttgtc atatcatgaa ccattccacc tacacacttt gacatcaaca atgccttatt
      121 ttgtaagtct ttctcatcat ttgcataaaa tattggacaa cctccaccat ttttcatatt
      181 cttatcaagt gtaactatag ctagggtata ttcatttaaa cctatatcca ttcccatctt
      241 atgcacttcc tttctttcct attttataat tttttagtga taagtttttt cctttagagc
      301 ttgaaagtat tgggcatgac tttacagctt ccattacttt ttccatattc ttatctactg
      361 gtaaataagc aatcattata ctttcatcct ctttatttct tctaggtaat ggtacgaatg
      421 aaggttctcc ataatctcca tataaaccta accttgaata tatattgtat actattgctt
      481 gtctttgacc ggagtcaaat aatattcctg cattagcata atccttattt ttaggaatta
      541 tttgtattcc tacacctcta ttaatatata cttctctatc actttttaga cctatatttg
      601 ttataccttt taaatctccc acctttagta tagattcgtc cacaaaacta atttcagcag
      661 gcattacatc agctatgtct ccaatacttt ttctagtaag aagttttttt aatataaagg
      721 ctaacactaa tccacaaatt gaacttatta ccaaactcat cccttcactt attttcgttt
      781 ctgcaagtat tacataatac aatcctattg ttaaaaaaga tgtaactata cacatataat
      841 ttcttacttc ataggttcta gctatctctt ctatgaatgc agcacctctc tttacaagtt
      901 gaacttcctc taaattttgc aatgtatttc ttctatttgc tctcacttgt ctaaattgtt
      961 ctgctgctag agataaaaat gttatggatg tataagaacg atttataaga gctggaacca
     1021 ataatgctcc taatgaagaa gcgacgaatg ctaatataag ttgagatagt aaaacatttg
     1081 gttgcgttgg atactgtttt tggtctaaat tcaacattat aattctagat gttataccaa
     1141 taataacagc aacaaaaaat actctatcca ttaaatcact cctaaaatat ttaatttgaa
     1201 attttacatg taaatctaca aagtctttct agaaaaatca ctctgctttc cttttgatgt
     1261 ttctatgata ggtgtacttt tcactacatc tatcatgaca tctatatctt tgagtattgg
     1321 catatatggt ataaccacag tacttttttc taaattggtc ttagatatag caacaatatc
     1381 aacctcatct acatctctat ctatccccat atgaataaat agattatgta ttatagcttg
     1441 tctttgccct atatcattta ctattccaaa tgcacctaaa tctcttggta taacttctat
     1501 ggccagacat tcatttctat acttttctct tgtactttct agacctatat tagttattat
     1561 tacatcattt accattaaaa taggtccttc aaaacttatt tttgcaggaa ctatatctgc
     1621 tatatctgct atactgtttc ttcttaaaaa tcttctaaaa atcaagccaa caatagctcc
     1681 tgacacaata gctaaaattg tgcaatgaaa aaagctaagg tcatactttc ttgcaaaaac
     1741 gatataaact atcgaagata ctagtgcaga aaaaagactt atataacttc tagattcata
     1801 cgtagatgct atttcttcaa cataagcatc tccttttggt actaattctt cattatcaat
     1861 attctttagt gttattcttt cttgttccgc aagaccttga aactgttgta ttgctactgc
     1921 aaaaaatgtc aaggctgcaa actccttatc tataagtgca ggtaatgcta tagcacctaa
     1981 cgaagctgaa agcccagata taattatctg ttctaaataa tcttgtggtc ttgatgggta
     2041 ttgtttgtct gtaactctta aaactaatcc tctacataat attcctacaa ctaatgcaac
     2101 tataaaagag tgtctaaata aatttgcatt caataaactt tcttccattt tctcagctcc
     2161 ttctcttata tagtttgtgc aaaaaacaaa aaaaattccc ttctatttaa ggaaattttt
     2221 ttgtaatatt gtaactataa attcgctacc aatgccaagt tcacttttta cttttataca
     2281 tccatttagt gatttgacta tatgctttgt tatagccaat cctaaacccg ttcctcctac
     2341 atccctactt cttgctttat ctaccctata aaatcgttca aatattcgtt ctacatcttc
     2401 ttttggtatc cctacaccat tgtctataac tttaattacg attttttctt catcttttaa
     2461 cactacaact tgtacaatcc cattatcttg ggtatacttt atagcattat caactaggtt
     2521 taagaacact tgctttatat agtctttatt ggatagtata cttattgatt tatcatcaca
     2581 tttatataaa acttctatat ttttttcact agctgaatgt tgagttattt catatacctc
     2641 tttaaataca tcatatacta caacattttc cataagaaga gtatctttat tttctacaaa
     2701 tgataacagc aatatatcat caatcaacct ttttaatcta tctgattcgc tttctattat
     2761 ttctaagaat ctatttctag tattcacatc tatattttca tttgacttta gtgtttcaac
     2821 aaacccactt atagatgtaa gtggtgtttt aagttcatga cttacatttg caacaaaatc
     2881 acttctcata ttttcaagtt taactttttt agtaatatct tctatattta ctatcgaacc
     2941 tattattacg ttgttagtat tctgaagata aactgggtct acctttatct tatatacgat
     3001 atcatcttta gtagtaatat tagcagtttc attatcttta gaaccaataa attgaagtat
     3061 ttgttttaat atttcttctt cttttattac aacattaata ttatgacctt caactaattg
     3121 atttggttca ctctttatca tttcttttgc ttcatcattt atcagaagta tatttccatt
     3181 tacatctata gccaaaattc catgagatat actctttaat atagatctta actgtaaatg
     3241 tttatactgt acttcttcta aggttttatc catattcttt atcatatgat taaaatttct
     3301 agctaattca ccaagttctc ctttggctga tatgttaagc ctagcatgat attcattatt
     3361 acttactttt tttgagacgt atataaactc ttttaaatat ttccttaatg ttattgtata
     3421 tcttacagct aatataacaa tggctatgga caacataaca ataaaaaaat atatactatt
     3481 taaatcttcc acaagataac actcctaaaa tataaccttt ttaatctatt ttatacccta
     3541 cacctcttat agtctctata tatttttccg atttatcttc atcttctatt ttttttctta
     3601 aatatcttat atgaacatct actgttctag tttctccata gtattcatac ccccatattt
     3661 tatccaaaag ataatttctt gaaagtactt ttcctttatt ttgaattaat aattttaaca
     3721 attcaaattc cttaagagtt aactctatct tttcagagcc tttggtaact tcatgttttg
     3781 ataaatctag ttttaaattg cctgtagtaa gtatattgtt ttcttcacct aagctagtca
     3841 aattatatct ccttaaaaca gaactaatcc tagctaacaa ctcttttata ctaaatggct
     3901 tagttatata gtcatcagca cctatttcaa gcccttcaac cttgtcattt tccatatttt
     3961 tagcagtaag cattataaca ggaatatttt ttaagtcctt atcacttctt atttttctca
     4021 aaacttctat tccacttata tttggtaaca tccaatctaa aagtattaaa tctggtttaa
     4081 tttccttagc ctttataaat ccgtcaaatc catcatatga ataactaact tcgtaatttg
     4141 acacctccaa attgaatttt aatagctcaa ctatatgcat ttcatcgtct ataacaagca
     4201 ctttagtatt cattaaaaag ctcctctcta ttttatctgt agtaccgttc ctattcgttc
     4261 tgatgcctta ttatgttttc tataagagtg gaatttatct gctctacaac tagtgcataa
     4321 atttaaattt attatatttt cttttttaac tccactacat ctaagcatat actcattaat
     4381 tttccataaa tctaaatagt aacttccctc ttttattata tagaactttt catcattatt
     4441 tgtaagaatt gtgttaaatt tttctactaa atcttcagac acttcatagc aacatggtcc
     4501 aattgatggc ccaattatac atactaaatc ttcaggaact gttttatagc attcagacat
     4561 taattttatt gttttatgac ctatattact aaaagtccct ctccaacctg catgagccag
     4621 tcctatggct ttatttttag catctataat tgatataggt acacagtctg ctgtaaacag
     4681 taaaagtggt actttttcca agtttgttat aagtgcatct ccatctcttc ttttccctat
     4741 agtatcttta ttgactatat taactatatc tgaatgtatt tgagaattac tagttatatt
     4801 ttcaaatgtc aaatcacatt ctttacatac ttttaacata tcttctctat tttttgcatc
     4861 tatattctta catgttataa ctagatttac agaatttaat ttttcatcaa gttcttcaca
     4921 attaccactt aaattaacat actctttaat atcttgtaca ttttttatat ttatataatc
     4981 tttcatcatt tgtctccttt ttctatcagt atacttttaa gttcatttac aaaagtattt
     5041 atatctttaa attgtctata cacagaagca aatcttacat aggcaacctc atctaactct
     5101 ttcaatttat ccattaccat ctcaccgatt tttttggttt caatttcttc tataaaacat
     5161 ttatttattt cattttcaat atgtacaaca atactttcaa tctcttcaat agaaacaggt
     5221 cttttttcac atgcttttag caatccatat tttatctttt ctctatcaaa atattctcta
     5281 ctattatcct ttttaatgac cattaaagga gttgtttcta ttttctcata cgtagtaaac
     5341 ctctttttac atgattcaca ctcacgtctt cttctgatag attttaaatc tgtatgtcta
     5401 gaatctatta cctttgattc tttataatta caataaggac attgcatcta tattcctcct
     5461 ttgtttaact atatctcaaa ctcatctaag cttaattctg aacctatatt tactattatt
     5521 gtatcacaac ctattttaag tatatcattc caaaatataa cttcttcatc ccctcctctt
     5581 gaaaaaaaac ttctacttcc atctccaaaa atggtaaatg agactacctt accttcgtga
     5641 atatccatgt ctatgtctat tataaacccc atcctttttc cattttttac atttataatt
     5701 tctttttcca ttatatcaga aactctaatc atattgtact caccctttct ttaaaaataa
     5761 atatgtatcg aagtaaaaaa aaatgaccta tataggtcat ttaaattaaa ataatgttta
     5821 cagtccatgg attacttata catattttct catatttttt aaagcatttt tttcaattct
     5881 tgatacttgt gcttgagata taccgatttc atcagcaact tctatctgag ttcttccttt
     5941 gtaaaacctt aaatctaata ccaacttttc tctactattt aatttcttaa tagcttcttt
     6001 aagtgatatt tcttgcaacc aattttcatc agtatctttt ttatcttgaa cctgatccat
     6061 aacaaatatt gcatctccat tgtcttgata tactgggtca aacagagata ttgggtcttg
     6121 tatggcatca agtgccatta caactgattc tacttctaat tctaattcct ttgctatttc
     6181 tgatactgta ggttctttag aatttgttct aattaatctt tctctaactt gtagtgcctt
     6241 atatgctata tcttttaatg acctacttac tctaattggg ttattgtctc ttagatatct
     6301 tctaatttct cctataatca taggaacagc ataagttgaa aaccttacat tttggcttaa
     6361 atcaaagtta tctatagctt ttataagacc aatacaacct atttgaaata aatcgtctat
     6421 attttctcct ctgttgttaa acttttttat tacactcaag actaatctta aatttcctct
     6481 tacaaactgt tgtctggctt cttcatcacc attttttatt tgtaataaaa gttccttcat
     6541 ttgcttattt ttaagaactg gaagttctga tgtattaaca ccacatattt caaccttatt
     6601 aacttgcata tattttcagt cctctctcaa aagtattcat ctattgaaat tatttacatt
     6661 tccaattttt ttatactttt taagaagaat taatttaaca ttatacaaat tttttcattt
     6721 ctttttgcaa ccttgagatt attttctttt caagtctaga tatgtaggac tgtgatattc
     6781 caagcattcc agcaacttct ttttgtgttt tttcaatacc tttatcaata agaccaaatc
     6841 ttaattccat aatttgcttt tctctgtcac ttaatctgtc aagtgccata accaacaaat
     6901 ctctatctat ttcttcttca attattttgt atatttcatc attttctgta cccaatacat
     6961 cagataataa aagttcattt ccatctaaat ctatatttaa aggttcatca aatgatactt
     7021 ctgttttctt tttattattt tttcttaagt acattaatat ttcattttct atacatctcg
     7081 aagcataagt tgccaatttt atatttttat tcagtttaaa agtattaact gcttttatca
     7141 aacctatagt tcctattgaa atcaaatctt ctacatctat acctgtattt tcaaattttc
     7201 tcgatatgta gactactaat ctaagatttc tttctattaa aattgacttt acactttcat
     7261 ctgtttctag cttttgtaaa agctccattt cttcttctgg tttcaatgga ggaggaagta
     7321 tatttgcccc tcccatatag tatattccct ttggctttat tatttttaat ttaataccta
     7381 acatagtaat aaagcttatt attctttctt tcaatcgtaa cattccttgt cccccttaaa
     7441 ttttcaccta aaatccaggc aatatatttg gattcattat tgctccatat tctctatcat
     7501 taaaatttga aataccaagt attatatttc ctatcttttg ctcatctact tctatatagt
     7561 cagcttttaa gcctattatt atgcttgtct tattacttcc agcatgttta taaggtatta
     7621 ttctaactct acctgaagtt ttatcatcta aacttcctat aatttcttgt gctttcttca
     7681 tatctatatc ttcataatta atatttaata gtttttctgg aataaatcct tctaacacac
     7741 tagacttaac tattattaca tcactttgac ttattgggtc ttttaacaaa ttaccactat
     7801 ctactaaagc tttacagcaa aaactttttt ctagtaagtt tatatttata gtctttgtaa
     7861 gctccttaat gtacttaagc attttcaaat ctttataaat gtattttaat aattcacaac
     7921 tcacataagt acatgcaatt aagaaagata tttttaagtg acttattcct gtaaaataaa
     7981 ttataaaata agtgcttcca gaaataaaaa tgtttactag ataaaaaact aacgctacat
     8041 gaacataatc ttttttgctc ttgcttctaa atgaaattaa agttattatg ctcatgatta
     8101 ttattttaaa tggtacagta aatagtatat tcattgatgg atacaaataa gcaacagaat
     8161 aggcacttcc taaactagcc cccaataact tatttttttt aggattatat cttttagtaa
     8221 gaatagaagt acaacttata ataatataat ttattactag gttttctata acatagtact
     8281 caatatacac catgtatccc cccttaaaac ctttaaacat attataagat agtatgtaca
     8341 aaattttatg tcacttttga tggtcgaatc caaaacaaat tctatttaaa aagaacaatg
     8401 atatatgtct tatactttat gtcgtcaaaa gatatttttt taatgccaaa cagatagact
     8461 tttttgtaga atccaatata tctaggtaga agttattcgc tttacttttg gtatatttat
     8521 aaaaaaaaga ctacataatt taataatata tcaagtaaat cttttactta tataaaatca
     8581 aattatatag tctgtaaatt caaatataaa ttattgtgct agtaatccat ttgcatattt
     8641 tgcagattca tttagtgctt gttctggagt tatgtctcca gctatcatca tttgaagttg
     8701 tttccataaa tattcatata tctctactcc atgaggtcca actgtcaatg gtctatacat
     8761 atgtttatct cttgtcacca tttcttcaaa tcttgggtct ccttggtatt tttcaccttt
     8821 agcaactgga gctctaggta tttctttgtg gaaagcttcc attgattctt tgcttaacat
     8881 atactcaaga agtttaaatc caagttcttt atttttacat gctgacatta atgttaaatc
     8941 atctactgaa gcaaaacttc caactgtctt atcttctaat gatgttacat atccccagtt
     9001 taaatccggg aatgatttat caaatgtact tgtagcagca gaggatagca ttatagtaaa
     9061 tgcagctttt cctggtccaa atacactttc aatcatttca ttactatctt ttgataaagc
     9121 atcttctgga atatactttt tcatatcatt tataaagttt gcagctttca agccttctgc
     9181 attattaaat ttaactgatt ttaaatcatc attatatata tctccaccag cttgccataa
     9241 atacggatac cagttccagt ttaagcttcc aaatacttta gaaccccaac cttgtgcaag
     9301 accccactgg tcgatttttc catctccatc agtatctttt gttgcttttt cacagattct
     9361 tttaaaatca tcccatgttt ttggaggttt ttctcctaat ttttctagta tatctttatt
     9421 gtagtacaat acgcctggat tagcagcttc tataggtaaa ccataaatac cacccatcat
     9481 ttttgcatca tctaagtata gataattatc tgaagttcct gatttttcta aataaggtgt
     9541 taaatcttct actgcaccca tttctataaa ttgagggaac atctctgcat acatgtatcc
     9601 tatatctgga ccttcacctg cacttattgc tgttgcatat ttttctgcgt agttatccca
     9661 tggtactatc tgaaattcaa tttcacagtt attttctttt tcaaattttt caaatatcgg
     9721 cttccatgca tctgcatcat ggtctgttaa tggtgcagac catacaacta atttttcttt
     9781 accctctttg cctttagaat cttctttttt tgaacatcct gcaagaattg atactgatag
     9841 aatccctacc ataactaaag aaagaacctt ttttagttta atcatgctta atcccctttc
     9901 agactttaat ttgtcttttt cctcaaatgt ttacatttaa ttcagattta atattaacaa
     9961 ataagactac tcttgtctaa tcataaaaaa ctatactaac tcctataact atattttttt
    10021 tctataccta ttagatttaa ttcatactaa aaacttactt tttaaatttt atcaatattt
    10081 aaaaatattt ttaagtctag ccacaaacta actatctaaa ttgaacaaaa taaaaagaag
    10141 tctattgact tctttataag cttacatatt atcttcttct tcttaggaat gtaggtatct
    10201 ccatgctctc atcatcatct tcttcaatta tacttgcctt tttaggaggc tctatttttt
    10261 cctcagcaat tacttcttct tcaatttctt ttacagcttg ttttactgtt gtatttaatg
    10321 aagaacgtat gcttgattta ggtttagtat ctaaatctaa ggcaccattt tgtaatcctt
    10381 caaatcctgt tgcgattact gtaatcatta tttcatcacc taaatcttct tttatagaag
    10441 ctccaaatat aacatttgct tctgggtcac aggattccat aactaaagtt gatgcctcat
    10501 taacttcaaa taatcctaag ttaggaccac ctgtaacatt aagaagtact cctttagctc
    10561 cttgtattga tgtttctaga agtggagatt gaatagcttg tcttgcagca tcaattgctc
    10621 tagtttcacc actagcacta cctataccca tatgagcaag tcccttgtct ttcattatag
    10681 tagtaacatc tgcaaagtcc aagtttatta acccttcaac tgctataagg tctgaaattg
    10741 attgtatacc ttgttttaat acatcatctg caactgcaaa agcatctaac attgatgtat
    10801 ttttttgtac tatctgtaaa agtctatcat ttggtatcgt tataagagta tctacttttg
    10861 atttaagttc tgctattcca ccttcagcgt tcttcattct tatcttacct tcaaatgcaa
    10921 aaggtttggt aactacacct actgtaagta tccccatttc ttttgccaag cctgcaacca
    10981 ctggagcagc tcctgtacca gtaccaccgc ccattccggc tgttacaaag accatatcag
    11041 ctccttgaag taactttact atttcatctt tactctcctc agcagctctt ttccctactt
    11101 ctggatttgc tcctgctcca agtcctctag ttaatttttc tcctatttga actttatatt
    11161 cagcttttga tgtatataaa gcttgtttat ctgtatttac tgatataaat tcaacgcctt
    11221 ttagttgagc ttctaccatt ctattgacag cattgtttcc accgccacct acacctatta
    11281 ctttaatttg tgcacattct tctaattcta cgtcaaagtt tagcatcata aacccccttc
    11341 tctcatgatt caaagttatc gtatgtatta attatatcat tttcttcttt tattttgtta
    11401 acctttaagt aacatattcc acaaaaacgt aaattttcct ttttaaattt cttacttata
    11461 ttgtcaaata ttactcttta agtataaaat aatttacgta tatattaaat gctctttaat
    11521 ttttacgttt tttgttcata atattactaa taatatatcg tctaattata gataagttat
    11581 taaacaatct cataccaaaa actagtacag caacatagta aagagaaact cctaactttt
    11641 ctcctatata agtcaataat atagccaata tagcattacc tatgaaacca gtaataaaaa
    11701 ttacattatc atagttatct tcaaaattgg attttatcgc tccaaaaata gaatccatta
    11761 ttgcaagaat agctactgac atatatatgg aataatccat agaatatgta aatggaatat
    11821 agtaacctac tactacacca aatgctaatc ctcctaaaac ccacaacata aatcttcacc
    11881 tctttatttt actttgaaac acaactacat tacaatcctt tttattaatt ttttttactt
    11941 tttctttatt tttttgtatt tttcgtttta ttttgcaaat aaattcactt tattcccatt
    12001 attttcctga acttttatat taattccata tatatcctta agagatttag catatgattc
    12061 tggagagttt atagctgcac tcaataaatc tatatctcct atagctttta ttataaatgg
    12121 ttgtccatat gtagtatcat ttactgtaat agtagctcct gaacatttta tcttacttgt
    12181 tgctagtact ctttccccat ttatagaaat tgctcttgct ccagcctttt ttaaatcatt
    12241 taaaactctc aaaatatcta tatcatgtat aatcaaatca tttggattct gtccgtcttt
    12301 taactctctc tcactatccc tcatagtaat agttatgcct ggccctgtaa cagttgaata
    12361 accacttaat tctttaactt cttcaagctc tttttgcata agtgtttgga tagattcatt
    12421 tccattcttt tttgcttctt tatattcatt caaagttttt ttgttgtttt taagagtttc
    12481 ttttaaattt tttatttctt tttttgtatt ttctacctca ctttcaatac tttcagtttg
    12541 acctaatgtt atgtatactc tatttgggtc tagtgatttt atataagttg atatgaaaat
    12601 gcctaatatg aatgctccta atataactat tttctttttc atactattat ttcccctctg
    12661 ctttgtttat atcactatga ccatttattt ctatattgtc ttctttggtc acttttactt
    12721 tggcattata atatgtttct atttcccaga caataccata ctttatatta agtgccgaag
    12781 ccaatgtatc agaatcacca attgctttta ttataattgg ttcttttatg acagtgtcat
    12841 tcatacaaag cttgtcttct tttaaataca tatatgtatt aaaaactatt ctctggtcgt
    12901 ttatagatat ggcacttgct tgtgctgaat ttaatttatt tatcattgac aataataaat
    12961 cataattata tagtatacta tcatttttat cagagtttcc taagtctttt gtagaattta
    13021 tctcaatttt tatgcctgtt ccttcaacat ctgtatatcc agctaatgtt tcatattttt
    13081 ctatttctga cttaagtatg tcttgttctc ctttattgtt tttgcctcta taagtctcta
    13141 tatctttctt cacactatta acttctgatt gtagattctc tttttctgtt tttaaagatt
    13201 taagttcttc tgtaagttgc tctcctcttt taaaagttgt catccctcta ttttctatat
    13261 taataatttt aagttgtaaa ctaacaagaa cacccaatac aacacttcct atgattatac
    13321 ttttatttat aaatcttttt ttcaatatat cacttcctta caattgtcta tccttctgga
    13381 ctatacactg ccaactttcc atagttaaga tttatagtcc ctccattagt atttttattt
    13441 tgcaggtcaa tcagaatttt acttacccta gatatattat aatctaagtt atcatcatta
    13501 gaaagtaaga tttttatatt acttcttgta agcatttcta tattgctttc tttttttaag
    13561 tttacataat ttatttttct ataaattcct tcttctttta aataatttaa agttttaaat
    13621 acattctcct tagttttata atttttaaat tttatagatt tattatcatc aacgctataa
    13681 tctacatcta ctatcaagtc ttttttactt tcattagaac ctcttagttc ttccaataaa
    13741 tttccttttt tatctatata acaataatta tcttcatctt ttaatactgc aaatatttct
    13801 ttttctttta aacttataat tatcttattg ggaagttttc tttttatttc cacattttct
    13861 atatatggat tttttataag tttatttttc atatctttaa aattgtatgc aaaaatattt
    13921 tcatttagat ttatatttag ttctttcatt atatttgatt tggtcaccct cttatttcca
    13981 attacatcta ttttctttac atcaaataaa tcacttttta caattataca tatacaaaaa
    14041 gataacataa gtataaaaac taaaactatc atgacattat tcgtatttaa ctttcttctt
    14101 tttttcaaat ataatcaacc cctttggtta ataaatacac ctttagagct tgtttttcct
    14161 acaaaaaaat tataatattt aaagtcaaat ttttgcattt tattacataa catccaaata
    14221 aggtattaaa ttttataaat aaatcaatat ttcatcaaaa taaacatagt atactacata
    14281 ctatttatat atttaaatct gtatacatac aaataaaccc ctaatttata ataatttaga
    14341 aaatctaata tattataaat taggggtaaa tatttactta tcttttcttt attcctatta
    14401 ccttagcttg accttctata cttggagtcg tttctttttt tatttctttt acttccttaa
    14461 taactttttc cttttttgtt tttttagaag ttgacttttg tgttgaattg tatactttca
    14521 ttatctcatc atatataagg tctatggctt caggcttacc tatagtttta cttgcatttg
    14581 ccatatcaac taaaagttct ctatctccta aaagtttaaa tactgctgta tttaggctct
    14641 ctggagttag atttttttct aaaatagcaa ttcctgcccc ttgtttttct atactcttgg
    14701 cattatactc ttgatgattt tcagcagtat aagctttagg tattattata gatggctttc
    14761 caagagccgt tatctcagct aatgaaattg ctcctgcgct acctataacc aaatcacttg
    14821 ctgcaagtgc atttgccata tcttctaagt aaggcacaac tttttggtat ggtttaagat
    14881 ttatatcgct aatacttccc ataaattcat cataatagct tttgcctgta gcaaatataa
    14941 aagctatatc ttcatttacc atattcttta ttacaagtct cattgcatca tttatttttc
    15001 ttgaacctcc actaccacca tagcaaagta ccattctttt ttcgtcactt attgatagat
    15061 tctttcttgc aatatttttt cttgataata aaatttcttt tctaactgga tttcctgtaa
    15121 aaactaattt atcttctgct gcctctggaa atcttttatg cgaatcttca aaacttgtaa
    15181 gtacctttgt aactgtttta gataaaatct tattagttac accaggaaaa gaattttgct
    15241 catgtattat ggctggtatt ttccccatag atgcattaaa taaaacaggt ccactaacat
    15301 atccaccagt tcctataact atatctggtt taaatttctt aacaatcttt cttgattgct
    15361 ccaaaccttt aaaaagctta aaaactcttt ttacattatc gaaatctatt tttcgcttaa
    15421 aaccttgaac tgttacagtc tttaattcaa atccatactt aggaactatt tctgactcta
    15481 ttcccttttc cgtcccaaca aaaattatct cagcgtctgg atgctcatct cttattttgt
    15541 ttgcaatagc tatagctgga tacacgtgcc ctccagttcc accgcctgat aataaaactt
    15601 tcatcaaatc atcatctcct gttaattaat tttgacatgc tttgagatgt ttaacactat
    15661 tcctacagct cccataaaga tggtgagtga agttcctcca taacttataa atggtagtgc
    15721 tacacctgta gctggcattg atgatgtcgc aactgctata tttaaagctg cttgtattcc
    15781 aatttgtgca cctataccta taacaaccat acaagcaaat acattagaag tcttcaaagc
    15841 tattcttaca catctgtaga ctaaaactac aaatagcata ataactatta tacaaccaat
    15901 caatcctaac tcttctccta ttatagcaaa tataaagtca ttttgtggct ctggtatata
    15961 aaaatacttt tgtttactct ttcctaagcc tagaccaaac aatccacctg aacctagtgc
    16021 atatagacct tgtattacct gatacccctt accaagaggg tcttgaaaag gatctaaaaa
    16081 tgatgtaaca cgactaagtc tatagggttc agcaattact aatgcagcaa ataaagcagc
    16141 cccactccct atcatagcaa atacaatttt catatccatt ccagcaacaa atatcattac
    16201 aaaagtaact agaattacag tacctgctgt tgacaaattt ggctgaagta ttatcaacgc
    16261 aaaaaatatc cctggaacta cgagaagtgg tacaacacct tttgtcaaag attttatttt
    16321 atcataattt ttttcaatta attttgcagt taatattatt gtagcaaact ttgcgatttc
    16381 tgcaggttga aatgttaaag ctcctattcc caaccatctt tttgctccgt ttgcttctat
    16441 ccctaaaggg gtcaaaacta acaataataa tactactgct atagctccaa ttgctgtagc
    16501 atttttcttc caaaaactat aatctattct tgatgtaata atcataacta taaatccaag
    16561 tacagcatat attacattct tttttagaaa atagtacgca tcattgtgtt taaatgaaga
    16621 ttgtataaaa cttgcactaa acaccataac tattcctaca aatactaaaa gcatagtagt
    16681 gtaaaacact actccatcaa attcagtctt catccttatg tcaatttgtt ttttcaaatt
    16741 ttctttaggc atccaactaa tctacctcca ttcttccatt taagtaagtt tagaggtaag
    16801 agattatttt aaattgttta cattatcttt gaagtctttt cctcttactt caaaactttc
    16861 atacatatcc caacttgcac aagctggtga aagaagcact atatctccac tttttgaaat
    16921 ttcataagaa gtcttaactg cttcttccat atcttttact atatgtatgt cattaaatcc
    16981 tttattttta gcacaatttt ctatatttga tgcagtttct cctaataaga ccaatgattt
    17041 aacatatgac tttgcagttt caagaagttc atcaaaatta cttcccttat ccatcccccc
    17101 tgctataagt attataggtc tatcataaga ctgtatagct tttatagtgg aatctggatt
    17161 agttcccttt gaatcattta catatattac attgtctaaa tttcttacaa actcttgtct
    17221 gtgttccact cctttaaatg tcataagtac atcctttata acttctaaat caattttgca
    17281 gacatatgct attgctatag ctgccataca attttctaag ttatgacctc caggtaaact
    17341 tacatcatct ttatttagaa atttgatttt atcatctata tctattatta tgtcattatt
    17401 ttctaaatat actcctccat ttactttttt agttcttgaa aagtaaatta ctttggcatt
    17461 gcatttatca gctaaagatt ttactacctc atcatcatag tttaacacac agaaatcctc
    17521 aacagtttgg ttcataaata tatttgcctt tgcttctatg tatttttcca tagtatggtg
    17581 tctatttaag tggtcctctg taatatttaa tattgcgctt actttaggtc taaattcatc
    17641 tatactctca agctgaaaac tgcttagttc agttactaac acagactctt cacttgaagt
    17701 ttctatagta tctattacag gatttcctat attaccaact acatatgtat ctcgttttgc
    17761 tcttgaaaaa atttctccta ccaaactagt tgtagttgtc tttccatttg tacctgtaat
    17821 ccctataaaa attggattat tcgatagttt aaaagctaat tctacctcac caattatata
    17881 cttgcctgaa ttttttaatt tcaatatgaa tggtaggtct agtggtacac ctggagacac
    17941 tacaaccata tctatatcat ctacatcttc tgggtgatgt cctaatatgt attttatgtc
    18001 atttatagat ttcaattcat caagtatatt tctcaattta ttttcatctt ttatatcatt
    18061 tacgattata cttgcaccca atttatctaa atgtttaatt gtagatatac cagtttttgc
    18121 aagtcctact aataaaactt tttttccttt taagtccatt tttcattccc cctagcccta
    18181 aaatactgct attatactta tccaagctaa aacaactgtt ataatccaaa aaataaatac
    18241 aactcttgtt tctggccaac cacactgttc aaagtggtgg tgtattggag ccatcttaaa
    18301 gaatctcttt cttgttgcct taaaatatcc aacttgaatc aaaactgata gagcctccgc
    18361 aaaataaatt cctccaatta taggtattat aagcactgag ttagttagaa ctgaaaatgc
    18421 tacaactgca cctcctaatg ccattgaacc tgtgtcgccc ataaacactc tagccggata
    18481 tgaattaaat cctaagaaac ctaaacatgc tccaacagtt gctgctgcta aaactgcgac
    18541 ttctgtattt ccagcaatag aacttgcaaa tagcataaag aatacagaaa ctattaatgt
    18601 tactcctgat gctaatccat ctaatccatc agtcaagttt acagcattta ctattgctac
    18661 gattataaac atcattattg gtatatataa tattccaaca tttatcacaa aatctgtaaa
    18721 tggtatcata agttgtgaag cacttgatga tgatgtatac tgataaaaag caacataaaa
    18781 agataatgca acttgaagga ttattttttg atatgctttt agtccaagtg atctttttag
    18841 ctttattttt ataaaatcat ctaaaaagcc aataaaacca aaacctgcaa tacatataag
    18901 acctactgcc atatcatgac taacttttac tcttgtaagt ccagttatta gaattgcaac
    18961 aatcattata attccaccca tagtaggtgt tccgttttta gctagatgtg tttggggtcc
    19021 atcatctcta actgtctgac caaatttaaa ttttctaagc attggtataa atattggtcc
    19081 tattataatt actattaaaa acgcaatcaa tgcggtgtac gtaagctctg ttattcctaa
    19141 catcataaaa ctcctctccc ttgtcaatta actcattagt ttaagcttat ttatttagat
    19201 attcgactat tttttctaat ctcatgcctc ttgagccttt tactaaaata gtgtctcctg
    19261 tttttactaa ttcatttaac ttgttaaaca catcttctct attctcaaag tgatatatat
    19321 tggctgaatt gaatcctaat tgttttgctt cttctccaat aaacactgaa ttttcaccta
    19381 tagttattat aatatcagta ttattcatag atgattttcc aactaatcta tgaccatact
    19441 ctgaaatttc acccatttct agtatatctc caagtatcgc tacccttcta cctttatatc
    19501 ttccaagtat ctttaaagca gcatccatag aatctggact agcattatat gaatcattaa
    19561 ttattgttaa tttttcattt tttattatgt ctagtctcat ttttgtccct ttaaaatttt
    19621 ttaaaccttt ttttatataa tctaaagaaa tatttagaca caaaccaact aatatagcag
    19681 acattgcatt atatatatta tgttctccta ttgttggtat aaatatttcc tcttttttcc
    19741 catttattac acatataaat gttaaactat cctcttccat agtataactt tcacaataaa
    19801 tatcattgtt tttattaaag ccaaatgttt ttaacttata tacatgttct ttttctttta
    19861 atgtagataa aaacttatca tctccattta caattaaagt atttgtttca tcaaagtttg
    19921 aagtaatctc catctttgct ttcaaaatac cctcttgtga acccagtttt tctacatgtg
    19981 ataatcctat attcgatatg actgcaattt taggatttac tatatccact aaatatttta
    20041 tttcattgaa tccagacata cccatctcta ttacagcaca ctcatgttct ttatttagat
    20101 taaataaagt caatggaact ccaaaatgat tatttaagtt tttttcattt tttagtatat
    20161 ttaactttgc agatatggca gagtaaatca tatctctagt agtagttttt cccacactac
    20221 ctgtaacacc tataaatgga atatcaaatt tttctttata gtattttgaa atatctccta
    20281 gagccaaaga tgtatctttt acctctatta aatttatatc cgaactttct aattttattc
    20341 cattactttc attttttata aaagttttac atccactatc atatgcaggc ttcataaatg
    20401 tatgcccatc taaattttct ccaactatgg ctacaaatgc attttgggca cttgcctccc
    20461 tgctatctat aactatatca cttacacagt cattataatc tccatatatt aacttcccat
    20521 tagttgcaag tactaactct tttattgtta agcactccat tattaaactc ctcctttaaa
    20581 accttagcta tattctctta gcaacaaaag gcaatgtata ccgttatata tacattgcct
    20641 acgataactc ttaactttat aattatatac tatatttctg tttttttgaa gatgtttatg
    20701 ggtatatgtt aattatctgt tactttttag ttttcaaaat atagggttat acttgagtct
    20761 tcattaactt ttactccagg ttttgggaat atatccttta cctttgtatc ttctggaagt
    20821 tctatatctg catctaagtc tggttttaac ttcgcatcct ccaatgcctt tacagcatct
    20881 cctattttta gatttcttac atctggcact ttaacttgct tcttttcata ctcagctttt
    20941 tcttcttctt tatatactgg tttaactccc aaatatttta aagagtcatt cattatttct
    21001 ttcactattg gtcctgctgt agtactacca aatgcactta ctcctgttgg ttcgtcaaca
    21061 atagcaagaa ctactatttg cgggtcatca caaggtgcta ttccaacaaa tgaacatata
    21121 tactttcctg gagcatactt accatctata actttttgag ctgtacctgt tttaccacca
    21181 agtctatacc ctggtatata tgctatcttt cctccacctt cagttacaac tgattctgca
    21241 atttctaaca tttttttaga tgtttcttta gatataacac ttcttacttt ttttggtttt
    21301 actgtttcag ttatatttcc tttattgtca gtataagatt ttaccactct tggttgcatt
    21361 aaatctccac cattagctat tgatgatata gctgttataa gttgtattgg agttactgat
    21421 atagattgac caaatgatat tgtagccaat tcaactggtc ctacattttt ttcgttatac
    21481 agtattccct tagcttctcc tggtaaatct atacctgtct tatccataag gccaaatgat
    21541 tctatataat catacatttt tccaacacct aatctgcttc ctaattcaac aaatactggg
    21601 ttgcaagagt tttgtactgc ttgtttaaat tcttgtgttc catgaggtct gtaatgtctc
    21661 caacatttaa tctttcttcc tccaacagtt acactacctg tacatgtgaa tttttctcca
    21721 tctttaatta ctccctcttc aagagcactg gaagatgtaa taagcttaaa tgtagaacct
    21781 ggttcatatg tatcacttac tgctggattt ctccacattt gataatagcc ttttatttta
    21841 tctttatcat tatatttttc aagttcttct tgataatatg gatatattgg cgttcttgag
    21901 tcattagggt cataatctgg ttttgatgct aaagcaagta tatcacctgt tttagggttc
    21961 atagctattg cagtaacctt ttttgcatta tttaactcat aagctttctg tactgctttt
    22021 tcagtatagt gttgaattac ttcatctata gacaacacaa gaccattacc ttgtacaggc
    22081 tcataatatt tttccattcc ttgtggtatt tctcttcctg aggcatcagt acttactata
    22141 agttttccag gctttccttt taacttttta tcatattgca tttctacacc agatatacct
    22201 gttgcatctg aagaagtatg tcctaacaca tatggcgcaa aattcccata tgggtaatat
    22261 ctttgattat cttctgctac ccatattcca cttaatttag catctcttat ttgacttgcc
    22321 ttatcatcat ctatccatct ttttactttt acaagagcca tatttttttt gcttattaaa
    22381 gcgtaaatat ctttataatc ttcatccaaa atctcagcta ccttttcagc tgcttctttt
    22441 ttatcttcta cttccacagg tttacaccaa acagtatact tagttacact tactgctaat
    22501 tctttcatat ttctatcata tatagttcct cttttgggtt ctattggtat atctcttgtt
    22561 tgttgttcta gtgcttttgt actcaaccaa tttcccttca taagttgtag gtacccagtc
    22621 ctaataacaa ggcaaaaaaa caatgcacat gctagaataa gaacgagtac cagccttttc
    22681 ttacttatcc tctttacttt tctcaaatct attcccccta gttatccaca tctatatact
    22741 ttatttgtcc atcttttgga tagtccatat tgagcaattc cttagccttt agctcaattt
    22801 cttttattga tgttttttca gtttcttttg cctttagttc ctctaatact atctcttttt
    22861 ttcttaaatc tttttgtaaa taatagatat cgtacttcat ttccgagatt tttgaataac
    22921 cacatagatt caatattatt actgaaaaaa ttgcaatcag taaaatgcat ttagctccta
    22981 tttttttctt atttctcttt ttataattat tctttttttt atttttatat tcatttagat
    23041 tatgtatttt ataatttttt ttcaaattct caccccgata gtatatagtt taaattttcc
    23101 tctatactat atttgttatt aatcatattt cttcctctaa aatttcatcg ctaaattaat
    23161 acactattac acaatctaat tgtatttttt tctatacaca acaaaaacct ataaattcac
    23221 ttatactttt atatgaatct ataggttaga tattaatttc tatttatttt tatcacttct
    23281 agtattttta gataatttct tccataaata taatatgtaa ctaactatct actctctgat
    23341 tcctaagttc tccattatat tacttaatac catctatatc tatattttta tatctaaatt
    23401 ttcattatat atctcaataa caaagaacat ttcttatagt gttaattgtt ttttaattgc
    23461 tttaccagtt caacaaaata tataatactt tttaattaat tatagtaatc ttattttata
    23521 tataagatta ttctttagcc ttataactac ttatattttt tctgctactc ttagcttagc
    23581 acttcttgaa cgtggattta cttctatttc ttcctcacta ggtaatatag gttttctagt
    23641 tattattttc acctcagatt ctttatcaca ttggcatata ggtaaatgtt gtggacatat
    23701 acaatctgat gctaaatgct taaatgcatt tttcactatt ctatcttcta atgaatggaa
    23761 tgttattata catattcttc cacctttatt cattatagaa gaagcatcat ttatcatctt
    23821 tgtaattact ccaagttcat tatttacttc tattcgtatt gcctgaaaag ttcttttagc
    23881 tgggtgaggt ccatctattc tagctttctt agggatagct tttttaatta tatctactaa
    23941 ttttcctgta gtttctattg gtttgtttgc tctttcttca actataaatt tagctatacg
    24001 cttagcccag ttatcttctc cataatcttt tattatctta gttaattcat cttctgtata
    24061 agtatttaca acatcatatg cagaaaactc acacctaaca tccattctca tatcaagtgg
    24121 tgcatcttgc atataagaaa atcctctatc agcttcatca agttgatgag atgaaactcc
    24181 taaatcagct aatacaccat caattttgta aactcctatt ttttctaatt cctcttttat
    24241 attttcaaaa ttgctgtgaa caaacttcac tctactttca tattcactca atctctcttt
    24301 tgctgtgctt atagcatttt tatcttggtc aaaccctata aataatccct tatctgacag
    24361 tttttttact atttctttag aatgtcctgc tcctcccata gtacagtcaa catacacgcc
    24421 atctggcttt atatttaaat tctctataca ctcatttaaa agaactgata catgatgaaa
    24481 ttccatagtt acctccctta aattcacttt ttaaactatt cttcactaat attatgttta
    24541 tttttctttg ataaataaac atgtgcaatt atgccaccaa taactccaat tagacttata
    24601 acttgtgcca ttctaagtgg tcctaacatt aaactatctg ttcttaatcc ctctataaaa
    24661 aatcttccta ttgaatataa agttatataa gtaactataa cttgaccttc atatttttta
    24721 ttttttctaa atagtaataa aactataaat attccaaaat cccatatgga ctcatataaa
    24781 aaagttggat gtacctttac tccatcaacc attattcccc aaggtaaatt ggtagcacct
    24841 ccatgtgctt ctccattaat aaaatttccc catcttccaa tagcttgtgc taaaggcatt
    24901 ccaagtataa ctgtatcagc catttttaag aagttaatat ttttaatctt agtgtatata
    24961 taaccagcta atattccacc aattaaccct ccatgtatag caagtccacc acctctaaaa
    25021 ttaaaaattt gagacatatt ttgagcatag taactccaat taaatacaac ataatagatt
    25081 cttgcacaga ttagtccaac tggaattgct attatagcta tatttaatac atcatcatct
    25141 ttaattccca ctctttttgc ttcttttatt gctattaaag ttcctaaaat cattccacat
    25201 gccataagta ttccatacca catgatgtct atgccaaaca gtgtaaatgc tactctatcc
    25261 ataacgtcac ctctttgtat taattgaaaa tatttccaaa gcaatatgct ataattacaa
    25321 ttaattgtaa ttatactaaa ttatgataac acattaatgt taattctaca aaaaatatta
    25381 aaatttattc tacaaatccc tacactatat tatactaata taattactat aaaatttttt
    25441 aaactgaatc tctcatgact aaagttacaa gtgtacatag atatttttta atca
//
LOCUS       DACTGW010000037        25473 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.037, whole genome shotgun sequence.
ACCESSION   DACTGW010000037 DACTGW010000000
VERSION     DACTGW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.037"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(47..544)
                     /locus_tag="I9573_001186"
     CDS             complement(47..544)
                     /locus_tag="I9573_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT4795813.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNAIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVILAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            complement(719..1630)
                     /locus_tag="I9573_001187"
     CDS             complement(719..1630)
                     /locus_tag="I9573_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HAT4795814.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            2555..3406
                     /locus_tag="I9573_001188"
     CDS             2555..3406
                     /locus_tag="I9573_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HAT4795815.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            3410..3880
                     /locus_tag="I9573_001189"
     CDS             3410..3880
                     /locus_tag="I9573_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795816.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            3893..5203
                     /locus_tag="I9573_001190"
     CDS             3893..5203
                     /locus_tag="I9573_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005982059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4795817.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            5415..6161
                     /locus_tag="I9573_001191"
     CDS             5415..6161
                     /locus_tag="I9573_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT4795818.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCNSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            6523..8064
                     /locus_tag="I9573_001192"
     CDS             6523..8064
                     /locus_tag="I9573_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HAT4795819.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            8099..9205
                     /locus_tag="I9573_001193"
     CDS             8099..9205
                     /locus_tag="I9573_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAT4795820.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVTTEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DPLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(9650..10936)
                     /locus_tag="I9573_001194"
     CDS             complement(9650..10936)
                     /locus_tag="I9573_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT4795821.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(11059..14157)
                     /locus_tag="I9573_001195"
     CDS             complement(11059..14157)
                     /locus_tag="I9573_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HAT4795822.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGINDISHVK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKFKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFIVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMEVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFDDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDILTGLYN
                     YESFCEQTSLMLKRYPDKSYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDVHKLIYKIKNSLNRKKFEYMLVPCFGVYKILDNN
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLRHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            complement(15325..16029)
                     /locus_tag="I9573_001196"
     CDS             complement(15325..16029)
                     /locus_tag="I9573_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4795823.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(16020..17627)
                     /locus_tag="I9573_001197"
     CDS             complement(16020..17627)
                     /locus_tag="I9573_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HAT4795824.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(17881..19059)
                     /locus_tag="I9573_001198"
     CDS             complement(17881..19059)
                     /locus_tag="I9573_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795825.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(19285..19779)
                     /locus_tag="I9573_001199"
     CDS             complement(19285..19779)
                     /locus_tag="I9573_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAT4795826.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(19780..20541)
                     /locus_tag="I9573_001200"
     CDS             complement(19780..20541)
                     /locus_tag="I9573_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAT4795827.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            21264..24098
                     /locus_tag="I9573_001201"
     CDS             21264..24098
                     /locus_tag="I9573_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAT4795828.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHVSIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNISKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNIRKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            24141..25385
                     /locus_tag="I9573_001202"
     CDS             24141..25385
                     /locus_tag="I9573_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAT4795829.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKV"
BASE COUNT         8868 a         4037 c         3186 g         9382 t
ORIGIN      
        1 taaatgtact atactcaata cttcattcaa tatagtaata acaaatctac tttattccta
       61 tgtaaatatg aatttcttgt ttctttgtat caaaagtatt acattgatac tcttcaaaat
      121 cacttatata agtcctatca aaatccattt gccaaaactt aaaccaaaaa tctcctactt
      181 ctttttgacc tcctactata acaaactttg catacttacc tgctagtata actttactaa
      241 taatactttc tccaacagta ttattagtgt ttaattcttc taaattcttc tctttatcag
      301 aattactatt tacttcacaa catgcaacaa agctatacgg acttgtaaaa tcaccctggt
      361 aatctgtata tagacctatt gtcttgtcat tctttttacc ctttatagca ttatatatcc
      421 ctttgcccat aaattttttc cacaattcac ctatagcatt taccgcttgt ccatctttat
      481 ttgtagtctc cttcgatatt cctataacta ttttctcatt tatctctact atttcataat
      541 tcatacttta tacctcactt tgtttttatt aatatatttt attttgaact aattcaatta
      601 taatttataa tatacgacac ctttatgtca tatttttaat ataattaaac tcagtgagat
      661 attatatact aaatgcaaga tattatatat ttaataaaat ctttaatcta gcatcatatt
      721 ataatgaata aattttagac aactccctta gtctagttat tacattatcc ctaacatctt
      781 taggttcaag tacttcaaca ttgtcaccaa acgataaaat atatccataa atccattcac
      841 caataggaaa ttctgtaatt acatcatatg tatcatcttc atttttagtt attaaatcct
      901 tattaaaatc atcaaatact ctaaaaagca tcttttcttt aaacctcaat ttcaacgtta
      961 tagtattttc cttaattact ttagaatcat tcaaacatac ttcttctatt atctttcttg
     1021 aaaatacttc atcctttata gataaattcc ttatcctact aattctaaat atcctaaaat
     1081 catcttttag tttacaaaat ccatacaagt accaagtctt actcttatac ataagcttca
     1141 gtggttcgac agttctgttt gtctggcttg acaaactatt tacataatta aaatttatag
     1201 tattacgtct taaaatagct tcttttattt ttgtaaattt atcatcttca ttaaaattac
     1261 ttccccattc agtaaagtct atttcaatcc agtttgaaaa attctgctct ccaaatattg
     1321 agccaatttt atttactact gaatttattt ctggatactt agtcgcctgt aatgttttca
     1381 gtgcaactat taaactctct ttatcttttt ttgacagtat ggccttgtct atagaatatt
     1441 cttctattaa agatatccct cctcctttgc ctttcgtcat atatactggt actcccgaca
     1501 tagacagagt ttctatatct ctatatatgg ttcttgtaga aacttcaaac ctatctgcca
     1561 actctttagc tgttactaat tttttattta atagaattac tattatttct gttaatctat
     1621 ttattttcat tcattaccac ctactaaaat tattaaattt atttaatcta aattaatcat
     1681 actaattttt aaataaataa aatcttagaa tcttatctaa attattctta catgatttta
     1741 tttactttct attatctttc tcactttgga tattatcatc ttaatttatt tattactata
     1801 tcaatttact tattatcatc tcatttgtta tcatcttact taatttactt atttctatat
     1861 caattcactt attatcatct catttgttat cgtcttactt aatttactta ttgctatatc
     1921 aattcactta ttatcatttc atttgttatc gtcttactta atttacttat tgctatatca
     1981 attcacttat tatcatttca tttgttatca tcttacttaa tttacttatt tctatatcaa
     2041 ttcacttatt atcatcttaa ttcatttgct tattatcatt tactttattc aatttctata
     2101 tcaaaaatta gtatttgtac ttaatactta aaattaattt aaagtcttta catattattt
     2161 attttctatt gtaatattca tttccatact ttactaagct acttccactc atttatttta
     2221 aaatttcaat ttactatttc tcaactctat attattattt ttcaatttta tattactaat
     2281 tttaaagctt tttagctatt attcctacaa tatttaaaca attttttcat aataaagcta
     2341 tattacaata tattcaaaat atgtaatatt ttagatattt ttcgttgaca atgttaaatt
     2401 tttgttttat ccttaattaa ggacatgtta ttgacatttc tgaaacttat ttgaaatagt
     2461 tttttcattt tatttaggaa aacgtattat acttaagaaa acgtattttc tcatttcaga
     2521 taagtaaaat atttattctt ttggggagga aatcatggaa attaataaaa aacattttat
     2581 tcaagtattt ttaacttgct taatcttggt agtaatatgt gaatttatag ggcaaagaac
     2641 tatagctatt ggaaaagcct ctatagtact gttccctatg ctttatgcag ttataattgg
     2701 acttataata acaccagatt tattaggtaa aaaaattaaa gcacttaaaa aagtggttgg
     2761 agaaaaagaa ataaatattg ctggtgaagt tgttggaatt gcactagtaa tgcttggtat
     2821 aaaatatggt actacagttg gaccaaacat tgacaaaata attcaagctg gaccagcatt
     2881 tatcgctcaa gaatttggtc atatacttgc accaatcgtt gcattacctc ttgcattaat
     2941 gtttggaatg aaaagagaag ctgttggagc tactgcaagt ataagtcgtg aaccttcttt
     3001 aggagttata tcagaaaaat atgggataaa ctcaccagaa ggtagtggtg tacttggtac
     3061 atatttaatg ggaacagttc ttggtactat atactttagt gtactaggtt caatatctat
     3121 ttatagtggt cttcacccat atgctttggg aatggcctgt ggtgttggta gtggaagtat
     3181 gatgacagca gctgctggtt cattatctac catggtacca cctgaaatgg cagatacaat
     3241 acttgcctat gctgcaacaa gcaatatgat gtctagtata acaggaatta tattcttagt
     3301 ttttgtatcc ttaccattta ctaattttat gtataagatt ttagaaccta aattttcgag
     3361 aaataaaaaa actaaagaaa ttaaatctac agaaggagag tgctaataaa tgtttgctaa
     3421 tttaaattta aagaaaagtt catatcaatt attggttatg tctgttattg gagttataat
     3481 tcttatagga caaagaatta gcgttggtac atctatagtt acagctcttc ctggtatggt
     3541 gatgctaata ttagctgcta tggcagctat gattataaaa gatttatttc ctaaatcaat
     3601 ctttccagct tttggatttg caactataat cggcctttta cttagtatgc catatagtcc
     3661 aacatctgaa gtattcttaa ctaacactaa taatattaac tttatggcta taactactcc
     3721 actactagct tttgcaggaa tttctgttgg aaacaaaata gaagctttaa aagaaatgtc
     3781 atggaaaata gttataattt cacttattgt atttacaact atattctttg catgtgcgag
     3841 tatagctcat atagttttaa gtattcaagg taaaatatag gaggaattta ttatgaataa
     3901 agaacaatta aaatctctag taataaaaac tatagatgaa aatagagaaa aaatattaaa
     3961 aataggtcaa ggaatataca aaaatccaga atatggatat aaagaattta aaactacaga
     4021 agctgtttca aacttttttg agcaagaatt aaatttatct gtagaaaaaa atatagcagt
     4081 tacaggatgc aaagcagatg caaattctag caagaaaggt cctcatatat ctatacttgg
     4141 agaattggat ggtatatcat gtaaagaaca taaagatgct aatgaaatag gtgcttctca
     4201 tacttgtgga cataatattc aaatagctgg aatgttaggt gctgctgttg gtttagtaaa
     4261 aagtggcgtt ttagaacatt tagatggtaa agtatctttt atggctactc ctgctgaaga
     4321 gtttattgag ttggaataca gagagcaatt aaaaaaagaa ggaaaaataa cttattttgg
     4381 tggaaaacaa gaattaataa aaagaggtta ttttgatgat atagacatat ctatgatgtt
     4441 tcatgcttct gatttaggag aagataaggc tctagttggt cctgagagta atggatttgt
     4501 tggaaaaaag gttaaattta taggtaaaga atctcatgct ggttctgctc cacatgatgg
     4561 aataaatgct ttaaatgcag caatgcttgc tataaacaac gtgaatgcac ttagagaaac
     4621 ttttaaagag tctgaaagag ttagattcca ccctatcatc acaaaaggtg gagatatagt
     4681 aaatgttgtt cctgctgatg ttcatatgga atcatatgtt agagctagaa ctataaatgg
     4741 tatgattgat gctaatgaaa gaataaataa ggctttgatg gctggtggaa tggcagttgg
     4801 agctgatgtt gagataactg aaattcctgg atatcttcca atattacgat acaaaagtct
     4861 tgataattta tttaaaaaca atcttgaaga tttaggtcta aaaggaaaag ttattgatgg
     4921 tggagatttt acaggctctt ttgattttgg agatgtgtct catataatgc ctactcttca
     4981 ccctatgatt ggtggtgtaa aaggagctct tcatacaaga gattttgaaa tagttgatga
     5041 agatttagct tacataattc ctgctaaagc aatggcactt acagttgtag accttctttt
     5101 tgataatgct aaggatgcta aagatatatt agataccttt actccagtta tgactaaaga
     5161 agaatatcta acattcctag aaaaacacga taaaacttta taagtctatt tttataatag
     5221 ggtggtatct actgtaaaag ttataccact ctttgttttt gtaacagtat acttcttttc
     5281 ttttagcttt tagattaaac taaaatatat tatcctcctt aaaactttta gagatacgtt
     5341 aaaatatcag tagaactatg ctatattctt tctaattaat ataaatttat attctaattc
     5401 aaggtggtga aaaaatgttt aatgcaggaa atcgaattat aaatacatgg ttgtacccta
     5461 ttcaagatgg ctttgtcttg attgatactg gatatgaaaa tggttttaaa catttcaaaa
     5521 aacgaatttc taaattcaac ataaaaatca aagatattcg atacatattt ctaactcatg
     5581 ctcatgatga ccatgttggt tttctaaatc agattctaga cgaatctcct aatacaaaga
     5641 ttgttatgca tgataaagct ttagaaattc tatataaagg tcaaaactct tttattggtg
     5701 gttgtaacag ccgtactgcc tttctctttt gccaaattat gaaactattt ggaaaaggtg
     5761 agcatagttt ttctccactt aaacaagaat ttgaaaaaag gtgtattctt gtgtctgaca
     5821 agaatcgtaa agaaattgaa aaagaactaa atggaaaaat tatagataca ccaggacata
     5881 ctgctgattc aatttcacta ctaattgatg atggtgtttt attttgtgga gattctgcaa
     5941 tgaatggatt tccaagtact cacaaaatta ccatatttgc agaaagcaaa ctagaattta
     6001 ttcaatcttg gaaaacaatc atcaatacaa aacctaaaat gatttatcct ggacatggaa
     6061 aaccatttga atacagtaaa ttagaacaaa atctaatcta tgtaaataaa ataaatttat
     6121 atcctctaca taattcaaaa gatattcaca gatttttgta gaaaatacaa agtaaatttc
     6181 tttgattaat taaaaaatct aacactttac tacgctactt attttaataa cttaatcttc
     6241 cactagcttt tcataaaatt ttgttttaaa attaacttta aagtgcatat gctatttaaa
     6301 tacattagat ataattcgtc attaatctac ttaaagcata tttaactaac accactcaac
     6361 cagaaaattc agaaaactgc attgacacag gaaaaagttt attttatact ttttttagga
     6421 catattgtgg acacgtctta aatcgacaag cagaatttat attgtaagat caaccacaat
     6481 acttattttt taattatatt tttaaacatt agggaggata ttatgaatga taacgtagtt
     6541 aagaagaaaa acttttttga caggacatta aacagaatag aaactgttgg taataaacta
     6601 ccagacccag ttacaatatt tttagggtta tgtgtacttt tacttatact atctagtcta
     6661 gttggttcta tggggatatc agtagttcat cctggtactg gagaaactat aactgcagta
     6721 aatttattga ctgtagaaca gcttcaaatt cttttaggaa acatagttag caatttccaa
     6781 gggtttgctc ctttgggtct agttctagta actatgatag gtgctggtgt atgtgacaaa
     6841 acaggactta tgacagcaac aatcaaagca tcagtaagca agattcctga aacaagagta
     6901 actcttgttg taatgactat aggtatgctt gccaatatag cttcagatgc aggtacaata
     6961 ttatttccac cattagctgc acttgtatat cttggtgttg gtagacatcc acttataggt
     7021 ttattttctg gatatgcagc agtctgtcta ggatttgccg caaacattat gataagtgtc
     7081 aatgatatac ttgctgcaag ttttacagtt ccagcagctc aaatgattga ttctggattt
     7141 caagccaatg caacaatgaa cttatttttt atgatagcat caacttttgt tctaatactt
     7201 ttggcaacat gggtaacaga aaaaattgta gctcctagat ttggtaaata tgaaggaaat
     7261 gcagaactag atgttgatgg aagtcttagt gatgtagaaa agaaagggct taaaaaagct
     7321 ggtataagta tattaatata tgtagctatt atagtagctc ttagtgtaat cggtaaaaaa
     7381 ccattcttag ctgacccaga aacaggagat ttactatcaa acaatgctcc tcttatgaaa
     7441 ggtatggtac ctataatagc actagcattt ttcatacctg gtgttgtata cggaaaaact
     7501 ataggtaaaa taaagaagga taaggatgta gtctctataa tggctagtgc aatgagtgat
     7561 atgggtggat atataatact agcatttgct gcttcacaat ttttgcaatt attcacaaat
     7621 agtaatttag gtctgatact agctgtaaag ggcggagaat ttttaaaatc agcaggaata
     7681 aatggacctt tattgttgat aggatttatt atattatcat gttttataaa tttatttata
     7741 ggtagtgcat ctgcaaagtg ggctattctt gcacctatat ttgtaccaat gtttatgatg
     7801 gttggataca atccagcact tactcagatg gcttatcgta taggtgattg tataacaaat
     7861 ccacttagcc cattatttcc gtacttccct attatactag cattcacaag aaaatacgat
     7921 aaagatgctg gtatgggtac tgtaattgct aatatgatac catactctat atcattctta
     7981 atagtgtgga ctatactatt aatattgttt atggtattta atattccact aggacctgga
     8041 atacttcctt catatagcat gtaatttatt taaatctttc taaaaggaga aataatttat
     8101 gttagataaa aatagactta taaataattt tatggatatg gttagaattg atagcccatc
     8161 taatcaagaa ttagaaatgt ctaagtggtt ggtaaactac ttaaaagaaa gaaatataga
     8221 tgccattata gatgatgcag gtgaaaaata tggtggtaat acaggtaatg tcattgccta
     8281 tataaaaggt gaagaaggaa gtagaccact ttgtttatgt gcacacatgg accaagttca
     8341 accttgttta ggtgtaaaac caatactaga tggtaatgta gttagaagtg atggaactac
     8401 tacacttggt gcagatgata aagctggaat agctgctata cttgaagctt tagaacatgt
     8461 tactacagaa aaaataccac atagagatat ttatctttgt tttacaatct gtgaggaagc
     8521 tggtatgcat ggtgtcaaaa actttaatcc tgacaacttg ccttgtaaag atatggtgat
     8581 tttagattct ggtggagcta ttggttcgat agcatacaaa gctcctgctc aacaatctat
     8641 aaaaatctct tttcatggaa agaaagcaca tgctggtata gaacctgaaa aagggttaaa
     8701 tgctatatta gtagcctctc atgcaataag caatatgcac ataggcagaa tagatccatt
     8761 gactacttca aatataggta aaatagaagg tggtggagct acaaacatag taactgataa
     8821 ggttacattg actgctgaaa ttaggtctca tatacctgaa actttagagt atgagcttaa
     8881 tcatatggaa aaatgttgta aggatgctgc atctaaattt aatactactt atacctttga
     8941 acatagtatg tcttacccta gttttgaact tagtagagat agtcacgttt tcaagttaag
     9001 tgaagaagca ataagacaag ttggtgttgt tccaaatcca atggtaattg gtggtggaag
     9061 tgacgcaaat atattagcta atcttggata taattgtgct atattaagtc taggtatgta
     9121 tgatgtacac acagttaatg aatatgtaaa tatagacgaa ttatatgata ctgccaaaat
     9181 agtttatcat atgattaaat tataaatatc ataatatacc tctttaaatt ataaaaataa
     9241 aaacctatgt atcttcaact ttataagtta tatattaaag ttgttttaca taggttttag
     9301 tatcttatat aataatttat tttaacattt ttcataataa gctttggtat tttatatagt
     9361 aatttgttct aacatttttc ataataagct tcaatatttt tcataataat ttgttctagc
     9421 atttttaata acaagttcta gtatttttat aataatttgt tctagtattt ttaataacaa
     9481 gctttagtat tttacataat aatttgttct agtattttta ataacaagct ctagtatttt
     9541 atataataat ttgttctaat ttttttcatg ataagcttta atactttata tagtaatttg
     9601 ttgtagaatt ttataaaata taatgattta tacagtttta taaaatatct taaaacagta
     9661 tttcagtaat attttttggt cttcctccac caatactagt ctcttttgca catattcgac
     9721 ctaaacctgc atttactatt ttatttaata tacgtcttgc acttctttca cttacattta
     9781 gatactctgc taactctttt gaatcataaa tcttactttt tctattctca cttatagcaa
     9841 taatttttgc aactgactca caactcattc cagtcttttt agatatttct attacctttt
     9901 catcagatag catcaaagaa taatttaatt cattttcgga agctaaaggt cctcttatat
     9961 tttcatcttc atctaccaaa tatataccaa tttcttttga atctatggag tgtcttaatg
    10021 ctttatgtgc attattttca gcctgatacg cagttactcc agtaccaatt cctatactaa
    10081 gagaaaagcc catgtctttt atctctctct ttagattgaa tagcttatca taattatact
    10141 cattatcagc taccccttta tgaacaaata caacataatc atttcttcca agtaaaaata
    10201 aggaaccttg aatgcttctt acatagttta ctacaagctt atccatatct gattttttta
    10261 tcatgtcaga ataatagtta tctatcttct ctttataatc tattagactc aatatttcga
    10321 cagcaatttg agaaaatctt gccttgctta gagcatattc actctttacc ttatcataac
    10381 tttctttgat taattgtata gtagcttgta aacgaaatac aggatagcct ctttctttta
    10441 attcattata tactgcacca aaaccagtta acattatatc cgtcttattt tcttcaaata
    10501 attttatatg ccattctaca agttcagact ctcctacttc caaagaaaat ggattacaaa
    10561 atacactcgt tgctttaata tcaaattctt taagtgcatc ttccacaaca tatctatcaa
    10621 ctacatctat actgaattta tctaatttta tatttgcact ttttattgcc caaaatgcct
    10681 tcattatact cgtcccactt ctcggtacaa atgaatgtgg cttacttatc tcatacttag
    10741 attttataaa ttcataaact gcacatcctg taaatataat agcatcacat tcatcttcac
    10801 atttagaaat aacttctatt gtatctacta ctttttctct tacatatatt ttagtcttta
    10861 aatcactgtc tatctgtttt aaatcatcct ttatcttaaa cccagagtct gaaggaccta
    10921 taatacccac tatcataaac ttcgctccta atccataaat attatacttt ttaaaaatta
    10981 tatcctcact attttctact tctatttaat tatgttgaac tactccatcc tacctacatt
    11041 tagagtggga taattagctt atttttgata aagtaacttc tcaaaatcag cacttggaat
    11101 aggcttacaa taataataac cttgaattac cacacaatct aaactcacta aaaattcagc
    11161 ttgttctttt gtctctacac cttctgccac tacatccata tctaaatctt ttgccatggc
    11221 tatagtgtgc cttaaaacta ttttttctct gtctgtgatt ccttcctcat taaagaagcc
    11281 acaatctatc ttcaatgtat caatattaat attttttaac atatttaatg aagaatatgc
    11341 tgaaccaaag tcatccatat ttaaaacaat accatccatt ctaaggtcgg ctattatctt
    11401 agtaaataat tttatatttt taataaataa actttctgta agctccaatt ctaaaaaaga
    11461 ttttggtata ctatatttat ttattaaact taaaacaatt tctttaaaat gtggattatg
    11521 caaatgtaat cttgatacat ttacagagat aggaactggc tcttcaccta aatctatcca
    11581 ttttctaatc aaagaacata cttgttccca cacatacatg tctaatctga taataaaccc
    11641 attcttctca aatagtgaaa taaatttaat tggaggtatt ataccttttt ctggatgaac
    11701 ccatctaacc aatgcttccg caccaacaat tttatttgca ttaagattat acttaggttg
    11761 tagatataca tggaattgcc cattttcaag tgcgtattcc atttcccttt ccatctgttt
    11821 ttcttcaagt gcatcatttc ttatagtatc atcataaaat atatagttat tgatactttg
    11881 tttctttact tttttgtgag catatcctgc catttcacac gcatttgtaa ttggaatatt
    11941 attatccaaa attttataaa ccccaaaaca tggtactaac atatattcaa atttttttct
    12001 atttaagcta tttttaatct tataaattaa tttatgaaca tctctatcag agtaactctc
    12061 ataaaaaata caaaatacat ctgcatacat acgacaacat ataccattag aaccaatgtt
    12121 tttttgaatc aattctgcaa catatattaa agccttatct cctcctctaa ctccataaat
    12181 gtcattaatt accttgaact tatctatatc catcattata attgcatagc tcttgtcagg
    12241 atatctttta agcattaaac ttgtttgttc acaaaaactt tcataattgt atagtcctgt
    12301 taatatatcc ttttggtttt tatgtagttg cataagataa tctatatttt ttatagcaac
    12361 taaaatatgt gtttgtttct cacctaactt atttttacta ctaatcttta acatataaat
    12421 taatgcccaa tagtactttt tatctattcc taaacgtcta tactcaaaat ttagttctaa
    12481 atcgtcttgc ttgctgttat taagactatc aagagatttt agaaggtggt tatcatcaaa
    12541 taatattata tcatcaggat gaattaaatg tttttttatg tatgaaaaag ttttttcaag
    12601 attttccttt ataggaaaac taaaaatatt gctctctgat tgataaattg gagtaattaa
    12661 attagtatcg atgcaaatag ataaaattat atcatatgta ttataagcta atttttcata
    12721 agccttagaa taccctttta tcattaaatc atcaaaataa accttatcaa ctaactcttt
    12781 atgtttaaat aaaatattat tcgtcgaatc agctaagtca ctaattactt ccatttcatt
    12841 atttctaagt ttgttcttat tataatacct cttcttatct tggtacattc tctcatcagc
    12901 aactttaatt agcttatgta tatcaatatt atcgtcactc caaatactgc ccactgctac
    12961 actatatatc atctcatcct taaattcttc acttaaccaa ctcactaatt cttcaaatag
    13021 ttgttttgat atatcttcac ataatacaat aaactcatct cctccaatac ggtaaacatt
    13081 ataagaatga aagtattttt ttattattac acatgctgac acaatagcag agtcgccatt
    13141 agcatgtcca aagttatcat taatatcttt taacccattt atatctacaa atacaactcc
    13201 tatggattta aattttcctt tttgaaaatt atctatatac ttaatatatt tgtttctatt
    13261 atatgcacca gtcaagaagt catagtagct catataattt aatttatttt ctacttttct
    13321 ttttttcatc tcagttaata aaaaataaga tagggatgtc aaaagagagg tatctccaac
    13381 agccttatgt ggattatcaa ctccaataaa ccctactact ttatcatctt gttttagtga
    13441 agatgtcatt aaactttcaa ttttttgagg ttttaaaatt tcatattcta ttggctgttc
    13501 ttcttttata ttttctatat catcaattat tacaaaaccc ttttgttcga ataataaaaa
    13561 ccaattcttt aattcattaa tatcaagatt tgttagtgcc tgtttttgtt catcaacacc
    13621 ttcattgcac cattcatatg tattagttac tttttttcta tcttctgata cttcaaaaac
    13681 atatgctcta tcagctttat gaaatttacc aatattggtt aatacaagat ttacagcatc
    13741 agaaaaatta gattctgtta taagatgttt aatacatcca actaataact cctttatctc
    13801 taatttttct gctattgact tacttatatc ttccttttct gtaatatcaa gagctatttc
    13861 taatcgtgca tgtttgccct cccatttaat taacttgtcc tttaatataa aatgtctatt
    13921 tgttcgagta ttcgtatttt cccatatgta atattcatct tcttttaaaa tagagtttgt
    13981 acaaaattcg cagggagaat ctttcccctg taatgcttta taacatttta catgggatat
    14041 atcattaatt cccattgcct ttttcccaat ttcattaata tataaaagtt catatgtatc
    14101 taaagaagaa acatatacaa tttcatccaa ctcatctatt aaatttatta catccattta
    14161 tatatatctc cttttattca ttttccagat tgaattttta aagcttttaa aattttgcta
    14221 tatatttggc aatgatatta tacttcttta tgttttaaaa tagtacctta ataaagttct
    14281 attttttagt aaatagaatt attattgtgt caatcgtaca atagagtttt gtaataccta
    14341 atttaatata tttataatta actatcttat tatgtgttta caattaattc tcatataaat
    14401 gacaaatctc cacctcaatt aagtattcca atcatatatg aagataatac ttcattcatt
    14461 taaaaatata aaactattat atattaaaat ttcttcaata ataaattatc tagattgtta
    14521 ctttgatata ttatataata tcacaaaggc tgatataaaa agacaaaaag ggtaattttt
    14581 ttatatcaaa aaaaatgtta aattgatatt atccatatat aaattgatat tatccatata
    14641 gatgctatat ttaacaattc tacttacttc ccttacactt attagcatat agttattaag
    14701 agtttgttgc aaaacaacac ttagcacact ttaaaaagtg tggtctaata agtaatcaac
    14761 gaagaagaca taataaagca taaaatatga tatacttttc ctgtcatcaa aactgatgat
    14821 tttactatta atatctgcta aaatatgctt aataaattaa attttagtgg gagaatagtt
    14881 atgaaaatat ttagtttctt gttgtttgta gtgggaatat gtttttgtat tgctggattt
    14941 atgggacatt acccagcatt tgctggtgca acattgagtt tttttgcaac tacaatgata
    15001 gctgtaagac aagtcaaaac taagaaaaaa tgaaataggc aaatcctaac tttacaagtt
    15061 aatattacat aagtaattag tctaaaattg gatacttttt ctattacaga aatacatcat
    15121 ccactttgta ttctcttaaa aaaataaaat agctatgtaa attcaagatt agatagatta
    15181 actctctatc catttcattg taaaatatac atagctattt gtattcgatt taaaatactt
    15241 agattttctt acataacaaa taatttctat ctgattatta ctcatattca tttagtaatc
    15301 actcagcaac taatcattta gtatctattc atccgccata cggtaaccta ccccaacctc
    15361 agtcaaaata tattctggct gagcaggatt cttttcaatt ttccttctta tacttgccat
    15421 aaaaactcta agcgactgat tttcactttc atagtaattt ccccaaactt tatctattat
    15481 aaacttatga gttaagactc ttcctgaata cttagacatc aactcaataa tcttatattc
    15541 gattggagtt aaatgtattt cttcaccatt aattgacaca atatgatttt cataatctat
    15601 ctttaggttt ttaacttcaa aagactttgg agcatcatta tttatattat cctgttgagc
    15661 tttatttcta agagccacac gtactctagc caaaagctct gttacactaa aaggtttagt
    15721 taaataatcg tctgctcctc catctaaagc ttccacctta tccctctcat gctctctcgc
    15781 tgaaacaaca attattttat tagtctttgc acatgcccta actcgtccta ttacctcaat
    15841 cccatcaata tctggtagtc ctaaatccaa aattatcaaa tctgggtcaa gagaatgtat
    15901 aagtgatata gctgttcctc cattatctgt ctctacacac ctataatttt gagtttcaag
    15961 agatactttt ataaatttac aaattggctt atcatcttct acaattagaa ctaatggctt
    16021 tatttccact tgtctttccc ctcctcatca tacataggta tgctaaatct aaatgtagct
    16081 cctccagaag gattattaaa tgcgacaatt tctccaccat gagcttctac tatagattta
    16141 catatctcca atccaagacc aatacctctt ctttcaagat ttctagactt attcttagta
    16201 taaaacctat taaaaatatt accgatatct tcatttggta ttccttttcc attatctgca
    16261 acttcaaata gcacttgatt tgactctttt tttacagtta gttctatctt agaattttta
    16321 ggtgtatgcc ttatagcatt atcaattaaa tttactaaca cttgttctat taataataca
    16381 tccacatgaa cgattattat ctcatctgga atattggttt ttatgtctct actattggca
    16441 aactttttga ctcttaatat agattcagct attatttcat caacaacttc tggtcgtttt
    16501 tcaatatcca atttcccttc atctattcta gtcatactaa gtatattctc aacagaatga
    16561 gttagccaac tcgtatcttc atatatattt ttcaataatt ctactctagt ttctttatct
    16621 atcacatcat cattttcaag tattgttgaa gacgaaccca atatacttgt aagtggagtt
    16681 cttaaatcat gagaaattga cctaagcaaa tttcctctta atctctctct ctctgcctca
    16741 agatttgctt ttttattttt ctcaaataaa ttttctctat caattgccaa cgcaacctgt
    16801 gttgaaactg cttctaatag tatcttttca ttttctgtaa taatatctat cttaaaacac
    16861 gcaacaccta taacaccaag aacatttgat tcttgacctt ttatagggaa ataatatgca
    16921 ctgctgtttt tgcaatattt tgtcccaatt ccaactgggt tacctgtcct aaaagcttct
    16981 tttatagctt ctttttctaa tactgaatgg aatatattct cacttccatc attgttacaa
    17041 atataaatac ttggcttacc tagattatta ttctcatctg caatagtcac tattacagtt
    17101 ctatttaata tatttactaa attagaccca caaaattcaa taatttgatt tttatttttt
    17161 gctttaagca acgttttgtt attctcatag agcaatctta tcattttttc tctcttatga
    17221 gaaagtctca cttctctttt tatcctagaa gttaaagtac ttgttataat tgctgccatt
    17281 agcataatac aaaaagtcat aggataatct tttctataag ctaaaaaagt atataatgga
    17341 tgcgtaaaga aaaaattaaa tgatagcact cctattactg aagaaagtat tccaaaaaaa
    17401 tatccatagg ttatacttgt agcaaatagt acacctaata tgtatactag tataatatta
    17461 gattcattaa aacctacctt ttcaaaaact actgatattc cagtagcaat tgtcataacc
    17521 aatatagttt ttatgaagtc cttaataatt atctttttac tgagatagcc atcttcatca
    17581 taatcatact cttttaagtc ttcatgttgt agtcgttcct ttttcattta ctacaattcc
    17641 tccttattgt tactgtttaa attggctcta ttttatataa tacaatttta tcataataaa
    17701 ttcaatcaat acatttaatt aatgtactta aattactaaa aggtgctgtc ttaaaatgaa
    17761 aataaatctc tatttcaaaa cagcacctat atatttaaat tactaattta gtttttacat
    17821 acttaaatca atagttttat tcaattttat aaattataat acttcaatta tggaacattt
    17881 ctatccaaca agcataaata taaagtgggc acatatacct gcacacaaac cacctatggt
    17941 atgcgaaagt atcgctttac cagccaatgg tcttgcatct aaagcatcca tcatacctat
    18001 gtgtgtactt aaatatccac tccaacacat acccattgct gtgaatactg ctatatcatt
    18061 aggagttata gcgcctgatt ttataaactc tggtactaaa gatattgctg ctccaacagc
    18121 tcctaatgca gttacaggga atgctattgc ctctggtgat gtgaatccaa ataatggttc
    18181 tattatgaag ctaatcttat caccaatagc aggtaataat tttatacctt catatgcagc
    18241 tcctgtgtaa acagcttgac ctgtagcagg gtctgtagat ggtccaaaag ttaataacat
    18301 aactaatgta caaactacta aaactcctgg tattatagcc atacccattt caacacctag
    18361 cttaccacct tcaagtatag catccaatgt tctttggaat acattaccat ctcttatttc
    18421 tctaaactct tcaagttttg cacctttatc atttatttgc ttttcagtat catttttagg
    18481 gtcataatta taatactttt tagtaaaagt caacataatt ctaacactaa taatactacc
    18541 tattattgcc ccaacattac ctattatagc tggtagtaca tattctttac cttgagctat
    18601 cataaaagtt gttacaataa gtcccatacc aaaggctgtt cctaaattac ataaagcagg
    18661 tacttgatac ttcttaaaat attgtgtaaa tgttttgtct ttagcaaatg gtataatagc
    18721 tggattatca gataaatatg ttgcaactac tcctgctata ctagcaccag gtaagcccca
    18781 tataggtctc ataagaccct taaatatctt attaactaaa gatattactc caaactctga
    18841 aagcaacgca ctaagtgcac ctgcaagaac tgccatagcc attattaaaa atactgtgtc
    18901 taataaaagc gcatgtgctg tactcattat tactttaaac atcattcctg cacccatgat
    18961 gcttcctaca tatccaaatc ctaccgccaa tagtacaata aatacgaagg tttccatact
    19021 tactgccttg actcttctct tatccgacag cttttccatc ttgaaatctt ccccctttta
    19081 attttaaatt aaggttccta acattggtac aaattttttg gtacaaactg agttctaaaa
    19141 cttacactcg taatgcaagt tgtcagtttt ttgaaataaa aaaaaattac gtttatagaa
    19201 taagaatatc ctctttaatt tggatactct tattctattt tatgttatta tattatcatc
    19261 cttttgttta aaagtcaatt ttttctattt ttgttgttta attttattta tactcttttt
    19321 atatttttca ttttctttta tctttgtaag cactccatat aagttgactt ttctaaatac
    19381 agtagttata aataccaatc ctaatgcaat tgaacttgca catgataatg tactcacccc
    19441 atattctaca gcttttatat aattattggt caaaacagaa tacatagtat tatatattcc
    19501 ataaccaggt actaatatta ttaaacaaca aacacttaat gtagttgctg gagtcatata
    19561 aattcttgca cataactcac aatatatcgc aaaacaaact gtagctataa aaaaacataa
    19621 gttttctgtt actcccatat ttaaaagaaa tttatatgaa aaccaagcta tacttccacc
    19681 tattcctgct agtattaagt tttttccttt catattaaat agcactccaa agcttaaggc
    19741 agtaaaaaaa gctgctatta cttctataaa caaatgcact tatatccccc ctaacttgaa
    19801 gtataatcca agtaccaaga acattcctac tgctatagat acacctacaa aaaatacttc
    19861 tgctaatttt gcaagaccag aaatcaactg tccatctaac aaatctctta atgcattagt
    19921 caaagcaagc ccgggaacta agagcattat tgctcctgct attaaatggt ctaacgtctt
    19981 tataaatccg agctttaaaa ttaaaattga acaagaagct ataataactg ctccaattgc
    20041 atttacaaaa aagtcattaa gtgaagattt acttaaatac ataataacaa acttagtcag
    20101 aatcccaaca gtaaatgcag ctataaattc ttccacattt cctccaaata atatcgaaaa
    20161 agttgctgct gcaatacctg caaaaaatat tgttattaaa tccgagtaat agttatcttc
    20221 acttatttca tctaaaattt tctcacataa ttcaatatct aaattattta tacttattgc
    20281 tcttgataat gagttaactt catgaaccaa gtttaggtct actcttcttg agcttattct
    20341 tttaattgct gaatgtaatt tcccatctac aaatacagat gccattacag ctgttggagt
    20401 tgcaaataca tctacgtggt ctattccaaa agattgacat attctagcta tcgtttcttc
    20461 aactctatag gtttctcctc cactttgcaa catagcttta cctgcatttg aggaaaagtt
    20521 taaaattcta tctatatcca caaaacatat ctcccattct tttatagtat tattcatatt
    20581 ttaatacaag atattattta tttaaactat tttaaataat tcatatccaa tatttacatt
    20641 ttatgaaaaa acttgttttt tatgcttgtc aaaatcaaat tttaagcttg tttacaccat
    20701 aaaatggctg aattaatatt tgacaaacac tttcaaccat ttatataacc ttctaaattg
    20761 tataactttt tactacaaat aaaattatat ctcttttaat tctttctctt gagtatttaa
    20821 gcttatagca acttcatatt tatatatgta caatataaaa cattattttt atgatacaaa
    20881 atataatttg taaacgtata tatgtattaa ctgtgtatat ataaattatt cttaacctta
    20941 tcagtttttt catatatctt catctatatc gaccatatat taattacatt gaccatatac
    21001 taataaatga tattacatag actgaagtat attaaaaaac ttaaatttag ttatttaaca
    21061 acataatact ctaatttaat ctataaaaat ccatacctaa tataatgcaa ctcttatttt
    21121 aacaccatct attacacaaa acaacatatt aaaaagtaaa atacaaaata tccactataa
    21181 accttatttt aatactgaat atatattata atatatttaa tgtagcttaa atatagttta
    21241 aacataaaga aaggaaatgg tatatgctaa gaaaagataa agttctatta tctctacata
    21301 aattatgtga aaatatgact ttaaaagacc ttcaatcaga taaaacagga ttcactacaa
    21361 tagaaattgc taaccattca tctcttgata gaagtaatac cagtaaagaa ttaaataacc
    21421 tatataatga aaaaaaagtt ctgaaaatat ctggtaagcc aattttattt ctacatgtta
    21481 gtattataga aaatttaatt ggccgtactt tgaacagtga tgaattttct ataaataatt
    21541 gtaatgagct acttaaatca agtaaaaatg actcagaaga tattttttca acattacttg
    21601 gacatgattc tagtcttaaa ctacctatag aaaaagctaa atctgctata ctctatcctc
    21661 caaaaggtct taatacgtta ctcataggac ctactggtgt tggaaaaagt acatttgccg
    21721 aaacaatgta caaatatgct gtagaaagta aaatcttttc ttctgaaagc aaattcgtag
    21781 tatttaactg tgctgaatat gcagaaaatg ctaacttatt attaagtaat ttatttggtt
    21841 atgtcaaagg ttcttttact ggtgctaata aggataaatt cggcatagtt gcacaggctg
    21901 atggaggcat attattctta gatgaaatac acagattacc accagaaggt caagagatgc
    21961 tttttctttt gatggacaaa ggcatataca gacgattagg agaatctgac attgtgcaca
    22021 aagctaatgt ctttattgtg tgtgccacaa cagaagatat acattcttct ctgcttggga
    22081 cttttttaag aagaatccct gttacaatca acctacctgc tttacaggat aggtctttaa
    22141 aagaaagatt atctcttatt aaatacttct ttattataga atcaaaatac accaatgcaa
    22201 ctataaaagt tcacaaagat gtaattaaat cattactcct ttatgattgt tttggtaata
    22261 taggtcagct taaatctgac atacaactta tgagtgcaag agccttttta aattacaaaa
    22321 ctggacttaa ggataatata gaaatagatt tatcccttgt tcctgattac atctatattg
    22381 gtcttttaaa attgaatgaa aatcgtagta atctaaataa attatcagaa cttgattata
    22441 tgctttttta tgaatttaat ggtaataact tagaagttga atatacaaaa gaactatcta
    22501 aatacaatat agatacatca aacaatggat ttaaatactc tacagagatt ccttatctgg
    22561 aaaatgatat aaggagtatg tttataaatt acatggaaaa gtactctaca aaattacttt
    22621 cgatatcttc agtaactcat tctgattata caagtaatga aatttttaaa gtaatcaatc
    22681 caaagattta ctatgctgtt gaacattctt taaaagaggc cgaaaaaata tcgagaaaca
    22741 aatacacaaa acaaacttat attgctcttt caatgcatat aagtgcactt atagaaaata
    22801 taaatcaaga cacctataat cctaagtaca atttatataa tgataaaatt ttcttagata
    22861 aagatttaaa tatatctaaa atagttttaa gtataattga aaatgactta caaacatctt
    22921 tacctaaaga tgaagtaaaa tttatatcca tgtttctaaa tagcatgtat cttgaaccaa
    22981 acaatcaatc tgaaagtata ggagtcataa tacttgctca tggaaattca acagcaagca
    23041 gtatgtgtga tgtagcaaat tcattgcttg gtacaaacca ttgtaatgca attgacatgc
    23101 ctcttgatgt aaaagtaagt accatacttg ataaaactat agagttggta aaagaatgtg
    23161 acgaaggtaa gggtgtactt cttttaacag atatgggctc tcttatagct tttggagaac
    23221 ttatttcaga aaaaacaaaa atcaaaactc gctcaattga aatggtatct actccaatag
    23281 ttttagaagc agtaagaaaa agtacattac cagaaatgac tttggatgaa ttagctaatt
    23341 ccttaagctc tttaaatcca tatattggac gagcagcaat cgattcaaca gaaataaact
    23401 atcctgaaaa gtcaagagag tatgtaataa taactacttg tataacagga tatggttcag
    23461 ctatgaaagt agcagacttt ataaattctt cacttaaaaa tattcgtaaa tacaatatta
    23521 atttagttcc tcataatata gaaagtttta aaatgtataa gaataaattt agtaatacta
    23581 aaattctggc agttgttgga agtgtagatt ttaatgtagc caacacccct tttatacaca
    23641 tagaggaatt actaaatgga tatggcctag atatgctaaa caaaataata gaaggtacat
    23701 tcaatgccaa tagtattaca aatataaaag aaacaaatct atcatacaac agtttaataa
    23761 taaactttat gacccaaaac ttagaacttt tggactctgt taaattattc aactatgtca
    23821 attcttcctt atttacaata atagaattaa tagatgaaaa cttaaagagc aaatttaagc
    23881 taggatatat actacattgc tcttttatga tagaaagatg ttttaaaaat acaacttttc
    23941 catataataa cattactgaa ttaattagta aaaatcatga actatatagt ttaatacgtg
    24001 aagcctttaa gccaacagaa gcttatttta atattttgat accagacgaa gaaattgctt
    24061 atataataga catgattaat gagtatctaa ataaataaaa tttatagcac ttattttgtg
    24121 catattaatt aatatatgaa atggaggacg agattttgaa aggaaaaata aaacagttaa
    24181 caatacttgc attaattttt atatttataa ctcctgtatt tgcatttgca gatactccac
    24241 cagtaccaaa ttcttcaaga gcagcactac ttatagacca ggaaactaaa agaatattat
    24301 ttgaaaaaaa cattgatgaa aaaatgccat tagcgagttt aagtaaaatg atgacctttt
    24361 tattggcaat agaagcagtt gataagaatc aagtaaaaga gactgatatg gtgaaaatag
    24421 acaagtctac agcttcagta ggaggttcta cttgtaaact taaagatggt gatgaaatat
    24481 ctctaggaga gcttatgcag ggtctaatgc tagtatctgg aaatgatgct gctatagcca
    24541 tagctaaaca tattggtaag actgaaaaga attttgtaaa tatgatgaac aagaaagcag
    24601 aagaaatagg aatgattgat acgtattatt ttaatccaaa tgggcttcct atatatactg
    24661 acccagaaca taaagaacct cctattgaaa atatgtcaac tgctcacgat atagttacac
    24721 ttggaaagta tatgtatgac cattatgaaa atcaagttac aagaataacc actatgcaag
    24781 tatataatga tactaaaaag gattttacac attataacac aaatccccta cttgtttcag
    24841 tacctggtgt tgatggaata aagacaggct atacagataa tgctggatat tgccttgcat
    24901 tttctatgat ggtacctaaa gatgctaaaa acgaaagaaa tcatagattg ataggtgttg
    24961 tacttggtga tggtaacaag aaaaatagaa tttcttcttc cgctacttta ttgaaatatg
    25021 gtaaagataa tttccattct aaaaagatag cacataaggg tgatattata gagactcctt
    25081 gtgttgatgg tattgatgat tttaagataa cagttaaggt tgataaagat ttgtatggtg
    25141 ttgtttcaga taacgaaaat atcaatccta aggtagtgtt taaaaatatg aattatccta
    25201 tccataaggg agatattgtt ggtgttgcaa agtattataa tgactctggt aaatttgttg
    25261 gaagtgttga tgtaaaaagt gaaagtaata taggatgtat tcctttgaag gataagatta
    25321 agattaaggt tgctaagata aataaaaaac ttgagattaa aaattctgtt tgttttaaag
    25381 tatagtaaag catactatta aaagagtata ttaaatttat aaatactcca ctttgtggag
    25441 tttttttgtt ttttacaaaa aattaaaata gtg
//
LOCUS       DACTGW010000038        25221 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.038, whole genome shotgun sequence.
ACCESSION   DACTGW010000038 DACTGW010000000
VERSION     DACTGW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.038"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..488
                     /locus_tag="I9573_001203"
     CDS             <1..488
                     /locus_tag="I9573_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT4795830.1"
                     /translation="YNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNFALVMI
                     DIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNKSGEEV
                     ADIIDNIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGRNKVVY
                     K"
     gene            519..2519
                     /locus_tag="I9573_001204"
     CDS             519..2519
                     /locus_tag="I9573_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAT4795831.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKEKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNVRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTKKEFEEILRILYERRESSSGVGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            2867..3112
                     /locus_tag="I9573_001205"
     CDS             2867..3112
                     /locus_tag="I9573_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HAT4795832.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            3147..3872
                     /locus_tag="I9573_001206"
     CDS             3147..3872
                     /locus_tag="I9573_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAT4795833.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEEENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            3894..5207
                     /locus_tag="I9573_001207"
     CDS             3894..5207
                     /locus_tag="I9573_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT4795834.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVCGENLKKIV"
     gene            5506..5952
                     /locus_tag="I9573_001208"
     CDS             5506..5952
                     /locus_tag="I9573_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4795835.1"
                     /translation="MGGETHFLSFGKDEYKISLEEQENAIESAKASDNSVKLIAFIDD
                     EIVGIATIDSNQKAKGKHVGVLGIVVKEKYWGIGLGKRLMLDLIEWCKSNGITKKITL
                     VTNEENYNAIGLYKKVGFEVESILKKECYYNGVYTDLIGMSLLLGI"
     gene            6041..8026
                     /locus_tag="I9573_001209"
     CDS             6041..8026
                     /locus_tag="I9573_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAT4795836.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTITLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIEEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            8250..9218
                     /gene="spoIIIAA"
                     /locus_tag="I9573_001210"
     CDS             8250..9218
                     /gene="spoIIIAA"
                     /locus_tag="I9573_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HAT4795837.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            9208..9729
                     /locus_tag="I9573_001211"
     CDS             9208..9729
                     /locus_tag="I9573_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HAT4795838.1"
                     /translation="MQIKIIIIALLVGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            9746..9943
                     /gene="spoIIIAC"
                     /locus_tag="I9573_001212"
     CDS             9746..9943
                     /gene="spoIIIAC"
                     /locus_tag="I9573_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HAT4795839.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            9959..10345
                     /gene="spoIIIAD"
                     /locus_tag="I9573_001213"
     CDS             9959..10345
                     /gene="spoIIIAD"
                     /locus_tag="I9573_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HAT4795840.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            10430..11635
                     /gene="spoIIIAE"
                     /locus_tag="I9573_001214"
     CDS             10430..11635
                     /gene="spoIIIAE"
                     /locus_tag="I9573_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HAT4795841.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            11695..12312
                     /locus_tag="I9573_001215"
     CDS             11695..12312
                     /locus_tag="I9573_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HAT4795842.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            12324..12878
                     /locus_tag="I9573_001216"
     CDS             12324..12878
                     /locus_tag="I9573_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HAT4795843.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKKQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            12905..13594
                     /locus_tag="I9573_001217"
     CDS             12905..13594
                     /locus_tag="I9573_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HAT4795844.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSDSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            13704..14066
                     /locus_tag="I9573_001218"
     CDS             13704..14066
                     /locus_tag="I9573_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAT4795845.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            14151..14657
                     /gene="nusB"
                     /locus_tag="I9573_001219"
     CDS             14151..14657
                     /gene="nusB"
                     /locus_tag="I9573_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAT4795846.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            14641..15711
                     /locus_tag="I9573_001220"
     CDS             14641..15711
                     /locus_tag="I9573_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HAT4795847.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            15708..16913
                     /locus_tag="I9573_001221"
     CDS             15708..16913
                     /locus_tag="I9573_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAT4795848.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVMVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            16915..17130
                     /gene="xseB"
                     /locus_tag="I9573_001222"
     CDS             16915..17130
                     /gene="xseB"
                     /locus_tag="I9573_001222"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAT4795849.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            17133..18020
                     /locus_tag="I9573_001223"
     CDS             17133..18020
                     /locus_tag="I9573_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAT4795850.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            18050..18487
                     /locus_tag="I9573_001224"
     CDS             18050..18487
                     /locus_tag="I9573_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HAT4795851.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            18521..20386
                     /gene="dxs"
                     /locus_tag="I9573_001225"
     CDS             18521..20386
                     /gene="dxs"
                     /locus_tag="I9573_001225"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAT4795852.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANNNED
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            20519..21331
                     /locus_tag="I9573_001226"
     CDS             20519..21331
                     /locus_tag="I9573_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAT4795853.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEYTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            21351..23030
                     /gene="recN"
                     /locus_tag="I9573_001227"
     CDS             21351..23030
                     /gene="recN"
                     /locus_tag="I9573_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAT4795854.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLKSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGSFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            23245..24111
                     /locus_tag="I9573_001228"
     CDS             23245..24111
                     /locus_tag="I9573_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795855.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECTIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGDISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAQLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(24202..24693)
                     /locus_tag="I9573_001229"
     CDS             complement(24202..24693)
                     /locus_tag="I9573_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4795856.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIVEAKKIGYTHMRLDTLPSMKSAQGLYEKFGFYDIELYTYNPIEGARYME
                     LKL"
     gene            complement(24754..25194)
                     /locus_tag="I9573_001230"
     CDS             complement(24754..25194)
                     /locus_tag="I9573_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4795857.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGCGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFKFNGELDYGGEKVMVLKSN"
BASE COUNT        10245 a         2531 c         4570 g         7875 t
ORIGIN      
        1 tatacaaccg attgaagtat tttaaagcac aaaatttaca tgatggtctt acaaatgttt
       61 ataatagaaa aagttttgat attatgtatg aaaagtacag agagaaagat gataattttg
      121 cacttgtgat gatagatatt gataatttca agctcataaa tgataattat ggacataaat
      181 ttggtgacac tgttataaag ggaataacaa ataccatttg tgttatgtta gaacaagatg
      241 ataaagtctt taggtacgga ggagaagaat tttctgtatt aataagaaat aaatctggtg
      301 aggaagtagc agatataatt gacaatataa gggttgcaat tgcaaataaa aagtggaatg
      361 atgatgtgac tgtgactata agtgcaggtg tagctcatat ttcagatagc aaagatgtac
      421 tagaagaagc tgataaaaat ttatataaag caaaacaact tggtagaaat aaagtagtat
      481 ataagtaatt atatactata taaataagag gtataaatat gaaacaatca atagctatag
      541 taactgataa aataactaaa ctatcatctt tcttagaaga aaatatacgt ttggttttag
      601 gtaattatat tgatataaac cactattatc tggaagtttt aaaagagaat gataaaatac
      661 aaggtgattt tgtactagta atgaatgatg atagattaaa cagtatgaga aagcatttag
      721 tacaagatag taagattata gtgataagga gaacacttaa agaaaatgaa atatatggat
      781 tattttcaat accaaatgga actgaagtat tggttgtaaa tgatacaaaa gaaactacct
      841 tagaaacgat aagcttattt tacaaaatag gagtaaaaaa tttaaagttt actccatata
      901 ttgaaggaaa aatttataaa aatatagaca tagctataac gcctggagta aaggaaaaag
      961 taccaagtta tataaataaa gtaatcgacc ttggtaatag atatattgat atatctactt
     1021 ttatagaaat aataaatggt cttagaattg atgttaaaga aatacgtaaa aatttaatgg
     1081 catattctga gagtttaata agtttagata atggagttaa agataattat aggcaactgt
     1141 ttttaaaaat agaagaacaa gatgttatat tgaatctatc aaaagatgga ataatattta
     1201 cttcagcaga tggtataata aacacattta ataaagaagc tttaaaaatt ttaggtataa
     1261 atgaaaatcc acaaggcaaa aacatagaag atatcatttc tgaagattta aatattttac
     1321 ttgaagacaa agaaataatt gatgaagtgg tatttacaaa taaaaaatat ataaatgtaa
     1381 ataaaaaaag tattttttct atggggaata aagctgggat atattataac ttacaagaaa
     1441 taacgtatat aaaaaaatta gaacaaaatc ttactaataa attaagagaa caaggtcaga
     1501 tagcaagata tacatttaaa gatataaaaa ctaaaaataa aaatatggta aagtgtgtag
     1561 aattggctaa aaaaatatct aaatcagacc tttctgtatt aataatagga gaaagtggta
     1621 ctggtaaaga attattatca caatcaattc ataatgcttc caatagaagt aatcaaccat
     1681 ttatagcggt taattgtgca gcagttccag atagtctatt agaaagtcaa ctgtttggat
     1741 atgaaaaagg ctcttttaca ggagctttaa gagaaggaaa aaagggtcta tttgaacttg
     1801 ctaataatgg aactatattt ctggatgaaa ttggagatat gcctccattg ctacagacaa
     1861 agttactaag ggtattacaa gaaaagcagg ttatgcctat aggttcccat aatgttataa
     1921 acatagatgt tagggtcata gcagcaacaa acaaaaactt actaaagatg attagagaag
     1981 gaaaatttag agaggacctg tattatagac taaatgtatt gccaatagat gtaccatcat
     2041 tgaggaatag aaaagaagat ataataacac ttatgaattt ctttttgaaa aacaatgtaa
     2101 gactttcgcc agaagttata actattttag aaaaatataa ttggccagga aatataagag
     2161 agttacaaaa tgttgcttct tatgtaagtc taatgtgtga tagtatggtt tttagagatg
     2221 acttaccacc atatataagg gtagaatgtg ctaacaatta tgaagatgga tttggttata
     2281 caaaaaaaga atttgaggaa atactgagga ttctatatga aagaagagag tcatcctctg
     2341 gagttggaag agggtttata atcgaaaagc ttatacagga agggattgag atttctgaaa
     2401 gcaaagttag aaaggtgcta tcttatttaa gtgaatgtgg atatatatca tctaaatctg
     2461 gtagaggagg aagtcaaata acctcagagg ggataaaact ttataataat ataaattaag
     2521 ctaagtattt gatattgaaa tcacatattt tgaatttaga tatttaaata ataaactttt
     2581 atatttatag agattaattt atactatgtc agaaagaata ttgtaattta taattaaata
     2641 ttaataggtt tttaaaatag gattagatag gtaataggtt aagttagaat aacccattac
     2701 ctatttttat ttgtaagaaa agtgtacata gagttaaaag taaaaaatac aactaataaa
     2761 actattgaaa acactagata aataataaat aaaaataatt ataaattttg gcatatatat
     2821 tgctaatcat agagataaga cgacaaaata attgggaggt ttaatgatga aatacaaaac
     2881 aacagggact tgtgctacag aaattgaatt tgaagtaaaa gaaaataaag ttacaaatgt
     2941 aaactttata ggtggatgtg atggaaattt aaaagggcta aaagtattag ttgaaggcat
     3001 gaatatagaa gatgtaatac aaaagttaaa aggaatagag tgtaaaacaa agccaacatc
     3061 ttgcccagac caactgtctc tagcattgga aaattatatg aatataaagt aatacataaa
     3121 acaatcgatt agttaggagt ttaattatga aaccaataat agggatttta ggaaatttaa
     3181 taataatgga aaatggtatg tttccaggtc ttgaaaggtc atatgtaaat aatgattata
     3241 taaatgcagt gctaaaaggt ggcggtagtc cagtaataat tcctgtaaat acagataaag
     3301 aagttataaa aaaacagatt gaaatggttg atggagtctt aatatctggg ggatgggata
     3361 tcaatcctca actgtatgga gaagaaacaa gagaagaaac gacatttata tatccagaag
     3421 tggatgaatt tgatttaatt gcaataagta tagcattaga acttaaaaag cctatattag
     3481 gtgtatgtag gggactacaa attttaaatg taagtttagg aggaacttta tatcaagata
     3541 ataatcttat agaaggaagt tatataaaac atacacaatc ttctaaaaga catgtagcta
     3601 ctcataaagt cgatgttaaa gaaggaagta ttttagaggg aattttagga aaacaattac
     3661 ttacaaatag ttatcatcat caaagtgtca atcagcttgg aaaaggatta aaagccatag
     3721 catactcaaa agatggtata atagaagcta ttgagaaaga ggaggaaaat tttgtagtag
     3781 gagtacagtg gcatccagaa atgatggtag attactgtga taaaatggaa aaactattta
     3841 aatattttat aaatatttgt tcaaatttat aatatacctt ggggggattg aacatgaacg
     3901 aaaagaacaa aatgggatta ataagtatta tacttttagg tattaatgca gtagtaggag
     3961 ctggagtttt tctattacca ggagatgcaa tgaaatcatt tggtgtagca agtatatttg
     4021 tatatatatt tgatatgttg ttagttttat ctatggcatt ctgttttgct gaggttgcag
     4081 gtaaattcaa taaaaatggc gctgcctatg tatataccaa agaagctttt ggagattttt
     4141 gtggttttga agtaggtctt atgaaatggg taataggatg tatttcttgg ggagctttaa
     4201 tagtaggttt tcctacatca ttatcagcag tatgggctcc agcaggtgaa ccacatatac
     4261 aaaaaattat aattgtagcc atgatagttg ggcttacaat tataaactta ttaggtgtat
     4321 ctctatcaaa aattgtacaa aatgttataa ctgtaggtaa actaattcca ttaatactat
     4381 ttataggaat aggtatattt tttataaaag gagttaattt tacttcttca acaatggtac
     4441 ctccaggagc tggagcaaca gagtttggag cagcagcact tttaatgttt tattcattta
     4501 caggatttga atctatagca gttgcagcag aagatatgga aaatccacaa aaaaatatac
     4561 caatagcaat tataagtgta atagtaattg catcaataat atatatatta aatcaagttg
     4621 tatgtgttgg tatactagga gattcgttat ctagtacatc tacaccagtg gcagatgctg
     4681 caagaatatg ctttggaaat atgggagctg gattggttac ctttggaaca ttggtttctg
     4741 ttggtggaat ttgtatgtgt ggtgcttttg taaatccaag aagttgtgta gctttagctg
     4801 atgataaaat gttaccaaga atatttgcaa gaaaagataa aaaaggtaca ccatatgtag
     4861 cgataatagc tactatgtta atcactatac caatagctct atctggaagt tttgctgaat
     4921 tagcagcaat aagtgcagtt gctagattca tacaatacat accaacatct ttatccgtat
     4981 tagtgtttag aaagaaaaga ccagaattag taggtacttt taaaactcct tttggagcag
     5041 tgattcctct aattgctgta tgtgttggaa catggttgtt attccaagca tctatgcatc
     5101 aattaattat gggattagga gctttggcaa ttggagtacc attatacttt ataatgaagt
     5161 cttataatag aaaagtctgt ggtgaaaatt taaagaaaat agtttaatta tgttataaga
     5221 atttattgag ttaaaattag acattggcag aaaattaaat acaaataagt atctataagg
     5281 ctatattaaa atattattaa ttttttattt taatatagcc ttatattgtt atcaataaat
     5341 gaaaaatatt gtaacaacag aagaacaata gtattatgtt tatagataaa aaacttatta
     5401 ggaggtaaat ttatgccaac aataacatta aaaaatggtg tagacgtact tattagagaa
     5461 ggtgtaagag aagatgctta aagcattata gatttttata atgaagtggg tggagaaact
     5521 cattttctgt catttggaaa ggatgaatat aaaatatctt tggaggaaca agaaaatgct
     5581 atagaatcag caaaagcatc tgataattct gttaaactga ttgcatttat agatgatgaa
     5641 atagttggga ttgcaacgat tgattctaat caaaaagcaa aaggaaagca tgttggagta
     5701 ttaggaattg ttgtaaaaga aaaatattgg ggaattggat tgggtaaaag gcttatgtta
     5761 gaccttatag aatggtgtaa atcaaatgga ataaccaaga agataactct tgtcactaat
     5821 gaagaaaatt ataatgctat aggattatac aaaaaagttg gatttgaagt agaaagcata
     5881 ctaaaaaaag aatgttatta taatggtgtt tatactgatt taataggaat gtcactttta
     5941 ttgggaatat aattagtgta cattttatct cgaaaaaagt atattaagta tggtataatt
     6001 aaaaaagaat ataacttaat ataattttgg aggacgaatt atgaaaaatc tgtgtaaaat
     6061 atcagatgat tatttaaaca gcaagcttaa gtatttaaag ttattatcaa aacaatatcc
     6121 aagtatatca aaagcaagta cagaaataat aaatttagag gctatattaa atcttccaaa
     6181 gggaacagag cattttataa cagatgtaca tggtgaatat gaaccatttg tacatgtatt
     6241 aaaaaatggt tctggtgtaa taaagagaaa aatagaagaa ttgttttcaa atacaataag
     6301 agacagcgaa aaaaaaatgt tagcaactct tgtatattat cctgaacaaa agttagacct
     6361 aataattaag caagaggaaa atatagatga tttttataga ataaatattt atagacttat
     6421 agaactttgt aaatatgctt ctagcaaata tactaggtct aaagttagaa aactgttacc
     6481 agaaaatttt aaatacataa tagaagaact tttgcatgag catgttaaaa gtgaacataa
     6541 agaagagtat tataaaagta tagttgaaac aatagttgac ataggaatag ctaaggagtt
     6601 tataattgct atttctacag ttatacaaaa attggttgta gatagacttc atgtaatagg
     6661 agatatttat gatagaggtc caagaccaga tataattgtc gataagctta ttgaacatca
     6721 ttgtgtggat attcaatggg gaaatcatga tatattgtgg atgggtgcag cctcaggtga
     6781 aaaaacttgt atagcaaatg cactgaggat atcagctaga tatgcgaatt tagacattgt
     6841 agaagacata tatggaataa atctgttgcc tttagccact tttgctatag aaatgtataa
     6901 agatgaccct tgtaaagaat ttattccaaa ggttaatgac caaagtgtaa caacaacaga
     6961 aaagtcttta atggcaaaaa tgcacaaagc aataagtata attcaattta agcttgaagg
     7021 tgaggttata agacgaagac ctgaatttga aatggaacat agacttttgc ttaatatgat
     7081 aaactatgat gaaggtacga ttactttaaa aggtaaaaca tataaattaa aagatactta
     7141 tttacctaca atagacaaaa aagacccata taaattaact atggaagaaa gaaatgttat
     7201 tgataaatta gtatcttcat ttagaggtag tgaaaaatta caaaaacatg tttcattttt
     7261 gttttctaaa ggaagtatat atttaaaggc taactcaaat ttactgattc atggatgtgt
     7321 accattaaat gaagatggaa gctttatgtc tatgaatata atgggaaaag agtataaagg
     7381 taaggctctt atggataaga tggagtctct agcaagagaa ggatttttct ttaaagataa
     7441 ggcagaagaa aaactatatg gtatggatat tatgtggtat ttatggactg ggaaatgttc
     7501 atcattattt ggtaaagatg atatgactac atttgaaaga tactttatag aagaaaaaga
     7561 gactcataaa gaaaataaga atccatattt taaacttaga gaaaatgaga tggcttgtaa
     7621 aagattattt gaagaatttg atttagagct tgatgaatcc catataataa atggtcatgt
     7681 tcctgtggag agtaaaaatg gagagagccc aataaaagct aatggtaaaa ttctcgttat
     7741 agatggtggt ttttctaggg cttaccaaaa aactactggt atagctggat acactttgat
     7801 atacaattct agaactttgc aattagtatc gcatgaacca tttaattctg ctgaagaagc
     7861 cattgcaaat gaaagtgata tcttatcgac aactgttgta gtagagcata aggcaaagag
     7921 aaaaatggtg agagatactg atgaaggagt aaaaatacaa gaggaaatag aagatttaaa
     7981 actactttta atggcttata agaaagggct aataaaagaa atgtagatat tatagaggca
     8041 cttcataatt gaagtgtctt cttgttaata cttattaaat atattataaa gattaataat
     8101 agtagtttat attatactta ctaagtgaat ccaagataaa tgggttcact ttttttattt
     8161 ataaacatta ttaaaaaaat atagatgttt tggttatatt ccttgtcctt tataaataga
     8221 attatataaa caaaggagga ggacaagaga tgaataaact ttctgatgaa ataataaact
     8281 ctctttctac aactataaga gaaaaggtag aaaaagtctc aaacaataat ctgaacatag
     8341 aagaaattag gttacgctca caaaaaccat tgatactaaa tgcaaactct aaagattatt
     8401 tttacaatca aaaaactatg acacttgatt taaatcaaca aaactcatat gtagtaacaa
     8461 gagaagatgt agagcaaact tttcaaataa tatgtaaata ttcaatacat tcatttatgg
     8521 atgacataaa aaaaggattc ataactttga gaggaggtca tagagtaggg ttagttggaa
     8581 aagcaatagt agaagatgga caagtcaaaa atataaagca tatatcatct ttaaatataa
     8641 gggtatcaag ggaaattatt ggatgctcgg ataagatttt aagtcatatt ataaagggaa
     8701 aaaatcagat taataatact ttgataatat caccacctca atgtggaaaa acaactttaa
     8761 taagagatat agtaagaaat ttaagtaatg gtaatgaaga ttatggattt aaaggtttaa
     8821 aagtagcttt ggtagatgag cgtaatgaga tagcaggagc ttatctaggt gttccacaaa
     8881 tggatgtggg aataagaact gatataatag aaacttgccc aaaagatttg ggtattacga
     8941 tgcttttaag gtctatgtca ccaaatgtta ttgtgacaga tgaaattggg agtgaaaaag
     9001 aaataaaggc attatacaca gcattaaatg gaggtatagg attaataact actgtgcatg
     9061 gagattcaat agatgatata caaaatagaa aagaattaaa tagacttctg gataaagagt
     9121 tatttaaaaa ggttataatt ctttctgcaa aaagaggagc aggaacgatt gaaaaaattt
     9181 atgatttaga agaaaagaga tggtattttg caaattaaaa taattattat agctctcttg
     9241 gtaggaagta gttatttgat aggtgaacaa atatacaaaa cttatactcg tagacataaa
     9301 caattaaacg atttaataag agttttagaa attttgagga tggatttatc tttcggactt
     9361 tatacattag aagaaatctt taataggata ggtggaaata aagagttttg tttttggaaa
     9421 tttttctatc aaatatcaga aggacttcac aatgaacaaa gtaaaacttt ggaaataata
     9481 atatcagaaa atattgatgt cttaagtaaa gaaacttatc taggcaataa agaaattgag
     9541 gaactgaaaa atctaatact tactttaggg aaaagcgata tagagtcaca acaaagaatg
     9601 atagatttat ctatagaaaa tttgaaaaaa caaacctatg agacaaagga agatataaac
     9661 aaaaaaggcg tattgtataa aaaattagtg acattcatag gtataggtat atgtattata
     9721 ttgatttaat atggggaggt ttattatgga aatatcattg atattaaagg ttgcaggtgt
     9781 cggtattcta atatcagtat tgaatatgat attggaaaaa acagatagaa aagattgggc
     9841 aggtcttact acattagcag gtgtaataat cgtattgggt atggtaataa cagaaataag
     9901 tgacttgttt aatactgtaa gaacaatgtt tcaactttac taaaaaggag ggagtaattt
     9961 ggaaataatg caattaatag gaattgcaat catttcaact acactctgtt tagtaattaa
    10021 aaaagataga ccagagatag caaatttcat agcaataata actggagtaa taatactatt
    10081 gtcagttatg tttaagctaa attttatagt agatagtata caagatttgg ctaacaaggc
    10141 taacataccg actatgtata tatcattaat aataaaatta attgggatag catacttaat
    10201 ggaatttgcg atacaacttt gtaaagattg tggggaagga aacattgctt caaaactaga
    10261 atttggaggt aaaataatag ttatgtcaat gtctttccct atacttttgt ccattgtaga
    10321 aatggtggtg aatataattc catagatgtg tatcttggaa tgaatataag tatattagag
    10381 aactttcgtt gtattgaggt ttatagagtg aagagaggca tggttgctta tgaaaaaaag
    10441 atttttacca atgattatag gttttttatt tactttcttt tttgtaaata tctttgctat
    10501 tatgatattt gctaatgaag taccaagtag tgaagataaa gaatatggtg aaacaaaaaa
    10561 tagcatagat aaatatatag atggtcaatt agacaagcta gatataaatg agattcaaga
    10621 ctatataaat aaagaaatag ttattaatga tgttaattta aaatcatttg taaaagattt
    10681 aattagtgga gaaaaaaata tcttagattt attcaataaa gatggattga agatattgat
    10741 gtttgatgaa tttaaagcaa gtttaaaggt agtagcagta attttggtac tggctctgtt
    10801 atcatctatt ttaaagagtc tggaaaactc tttttcatca ggtgcagtaa gtcaaatagc
    10861 aacatatata atatttatca caatggtatc acttacactt attggtttta aagatgtatt
    10921 acagatttgt tatgatgcaa tagaccatac tgtaggtctt atgcaagtta taatgccaat
    10981 attaataaca tttttacttt taattgggtt cccaataacg tcgactactt tgaatcctat
    11041 cttcatagga ggagtaacat ttataaatgt attttttaaa aattttctat ttgtatctat
    11101 tactgttgca tttggaattt taataataaa caatttatca aaaaatataa ggctaaagag
    11161 atttttctcc tttgtaaaac aaataaatta tgtttctatt ggagctatgt ttactgttta
    11221 cttaggtctt gtatccatac aaggattgta tgtaaccagt tttgataaat tcagtgtaaa
    11281 gactgctaaa tttgctatag gtaattttat tcctgttgta ggtggatttg tatctgactc
    11341 tgtggatatt ttattatctt catctcagct tataaaaaat atatttggag gaatagggct
    11401 tatcttactt gttggaattt gtttacttcc agtaataaaa attttatcag taatagtagt
    11461 gtacaaatta gcagcaataa ttgtagagcc tgttggagag gatggaatat ctaatttttt
    11521 aaatgaagtg gcaaatttaa tgataataat gctagcatca gtaattgcta taacagtaat
    11581 gttctttgta actgtagcta tcttgacctc gataagtgtc gtaagtcaag ggtaatttat
    11641 atatcagtga aaaataatta catttaatct aatgttttta gagaggagga tgctatgtta
    11701 gagggcataa aggcatggat agttagtgtt ttgataggag catttatagt aaatatagta
    11761 gatatgattt taccaagttc taagataaaa ccttatgtca acctagtttt aaactttatg
    11821 tttgtattta ttgtaataac tccagtagta ggattttttt caaaagatat gagtttagaa
    11881 gatagaatat taaaatctat gggaaactac aacaagcagt atgtagatag tacaaatgct
    11941 ttggcaaaag agactggaaa taatagtcta tccaagggat atgaggatgg gttaaaagaa
    12001 gtacttaaat taaaacttga tgaatatggt tatgatttag aagatataga gcttaatggg
    12061 gcaaatataa ataatataaa aataaaagaa aagaataata gtgcaaaaag taataacagt
    12121 tccaatgaag aaaataaaaa taatagtact aaaagagagg aagaaaacat aaactctaat
    12181 gacaaagaaa attctaaaca agtcttcaaa aaaggtacag agtatgggtt gaacctaaat
    12241 gaagaaaaat taaaaaacga cttaataaag gtgctggatg tttctataga agatatacaa
    12301 attgataaat aggagttggg agaatgttga aaaacttaaa tgacaaagat aagagaaagg
    12361 tatattcact aataactata gcaggaatat gtgtggtttc attagtatta ttatcttgtt
    12421 ttcctagtag caaaagtgat aaacaagttg gaaaaacaga gactaataaa aatgctgaaa
    12481 agcaagtcac taaaaaacaa gaaaaagatg atttagagtc aaaattaaca gcaatactat
    12541 ctaaaattga tggtgcagga gacgttgatg taatggttac atttgaatca agtgaagaaa
    12601 tacaacctgc ttttaattca aataatacaa cagaaacaac agaggaaaaa gatgcacaag
    12661 gtggagaaag aacagttacg acttctagtg aaaataaaac aatgataact tcaaattcaa
    12721 gtgaccctgt agttataaaa actacagagc caaagataaa gggagtgata gtggtagcaa
    12781 gtggagcaag tgacccaagt gttaaagaga cactttatag tgcagtacaa acatcattac
    12841 aagtagctgg tcatcaagta gaaatatatt ctaaataaat acatataacc ggggggataa
    12901 atgaatgaag tttaattata agggaagagg atttgtaata ataactttaa ctgcaatgtt
    12961 agtggtagta ggaacagtga attatcaact aagtaagaaa tctttattag aaacatcaaa
    13021 agagtttaag gcatatgaag aagcacagtt gcaaaaaaat actgatgata gtgatagtag
    13081 taagacagag gattctagta atgtagataa acaaggtggt aaggaaagtg ctgatattga
    13141 tatagtcgat agcaaagcta gtaaagtaaa agaaaaggca actgagacaa gtaaggaaat
    13201 aaaagctcaa ttatcatcag agaaaaatat gaaaaaggca tcttatattt tagatatgaa
    13261 aatgaataga gaaaaacaaa gaaatgagtt agttcaagat ttaaatgaaa tgataaataa
    13321 tccatctaca acagaagaat ctagaaaaga agcttctaat atgaaattaa atatagttaa
    13381 aattcaagaa aaagaacttc aaatagaaaa ccttttgagt acaaaaggat atgaagaagc
    13441 gttagtttat ataagcgata acaaagttaa tgtagttgta aatgaagcta aattagaaaa
    13501 gaaagatgca gcaaaaatat ttgatttagt tgcagaacaa gccaatgtga agtatgaaaa
    13561 tattaaactt acaaataata atagtaataa gtaaagtata aacctcagaa ttaactgagg
    13621 ttattttctt gcaaagtaga cttgtatttg gtattattat acttataaaa atagttcctt
    13681 aaaatcagga ggtaagtttt aatatggaag ataataaatt tggacaagta aaaatatcta
    13741 atgatgtaat agctacaata gctggtttag cagcattaga agttgaaggc atagaaacta
    13801 cagcaacatt aacagataag ttgttaaaaa ataatggagt aaaaatacaa atcgaagaag
    13861 aggatgttaa tttagatgta atggttacga taaagtatgg aatgtcaata ccagatacag
    13921 cttttaaagt tcaagaaaat gtaaaaaata ctgttgagac aatgacagga ctgaaggttt
    13981 ctcaagtaaa tatacacatt caaggaatca gctttaagaa ggataaagtt gataaagaag
    14041 aagcaaaagc aactaagaaa aattaatatt agccctctga aaagtcagag ggttttttgc
    14101 tggtaattga ttttatatgg ataattaaac ttaaaggaga attttgattg atgaaaaaag
    14161 atagggcaca aaagagtaca acaagagaat atataatgaa acttatatac caaataaata
    14221 ttaataaaga agattttgaa actttagaag ataaagtaga taatttttta aaagataatt
    14281 cagaacatat tataaataga tataaagaac ttgcacttca atactcaaaa aatacaaatt
    14341 taaagttaga agatactgaa attgaagatg ttatagacaa gaaatatata aatacagtat
    14401 gcaaagcatt aaaagaaaat catgacaaaa tagatgagtt aataaataag catgctaaaa
    14461 actggacagt agatagaatg ccaaaggtag atgtttctat acttagattg tctgtgtgtg
    14521 aaatacttta tttagacact cctaataaag tatcaataaa tgaagcggta gaacttgcaa
    14581 aaatatattg tgatgataag tctcctaaat ttataaatgg tatattggga agtgttgttg
    14641 atgaaattgg gaaataatat aataattgga attgatacta gctgctatac tacttctata
    14701 gcagctatct ctttagataa gaaagttata ttcaatgaaa aaataatgtt agaagtaagg
    14761 gataattcaa aaggtttaag gcaaagtgaa gctgtatttc aacatatcaa taatttaggg
    14821 attcttagtg ataggataaa atcttttaaa gataagttta atgtagaagg agtttgctca
    14881 tcaaaaaaac cgagacctgt tgaaaactca tatatgcctg tattcaatgt aggtcataat
    14941 tttggaaaat tattgtctag tatatatggt tgtaggtttt atgagactac tcatcaagag
    15001 aatcatattg aagcaagttt attaaatagt aaattaaaaa ataataataa atttatttct
    15061 gttcatatgt ctggtggtac tacagagata cttttaacaa gcaaacaaga tagtcatcac
    15121 aatgtatgtg atactaattt aggtaaaatt gccaaaataa gtattaaaaa agatgataaa
    15181 agtaagttat acaataattt tggatataat atagatataa ttggaggaag taaagatatt
    15241 agctttggtc aactcataga tagagtagga attaagcttg gatataaatt tccttcagga
    15301 aagtatttgg atgaaaatgc tttaaattgt aatcttaaaa tagaaagtgg tctgaaaact
    15361 tctgtaagag atgggtatat gaatttatct ggattagaaa atcaggtaaa taagattata
    15421 aatgataatg gagataatac taatcaaaaa gaatacatat ctaaattagt attagattca
    15481 gtagttagaa atatgtttaa atctttagta tatttatgtg aaacttataa tgtaaatgaa
    15541 gtagtatttg ctggtggagt ttctgcaagt aagtatattc ttagagaatt aagcatgaag
    15601 ttaagaaaaa agcatataga agcttatttt acagaaccac aatattcaac tgataatgct
    15661 gtcgggtgtg ctataatagg attgaataac tttttaggag aaagagtatg aaactaagag
    15721 ctttagatat aagtgaagca aactcatata ttaaaagaat tttaataaac gacccaatac
    15781 tttctaatct taaagttaaa ggagaaattt ctaattttaa ggttcatagt agtgggaatg
    15841 tatatttatc acttaaagat gaaacatcaa agttaaactg tgtaattttt aaaagtaatt
    15901 ttaatcgcaa tttaaaatta gataatggtg taaaaattat agctaatgga tatatatcag
    15961 tctatgaaag agatggtgcg tatcaattat acattaatga aattgaaata gagggaatag
    16021 gaaatttaca catagagttt aatagattaa aggaaaaact taataaagaa ggattatttg
    16081 accctaaata caagatacct atacctaaaa tgccaaactc aattggcgtt ataacatctc
    16141 caacaggtgc tgttataagg gatataataa atgtaataaa aagaagatat ccgaaagtta
    16201 atataaagct ttatccagta atggttcaag gagataaatc agctgaagaa atctgtgaag
    16261 caattagatt tttcaatcat atgaagaatg ttgatacact tatagttggt agaggtgggg
    16321 gttcaataga agaactttgg tcttttaatg atgaaatggt agcaagagag gtttttaatt
    16381 ctcaaattcc gataatatca gcagttgggc atgaaacgga ttttactatt tgtgactttg
    16441 tgtctgatat gagagcccca acgccatcag cagcagctga gattgctact ccatcattgg
    16501 atgatataaa ctataaactt ggaaacataa aaagtaggat gagtaagtct cttacaaatc
    16561 aaatagagct agaccaatat aggttagaaa cagtatttaa taagataaat aactatttag
    16621 attcgtacac aataaaagat aaagttatac aattagacaa aatatatgat aaaataattt
    16681 ttggaataga aaataattta aaattagaag atgaaaaatt agtaaaaatt ggagcattac
    16741 tccataattt aagtccatta gctactatgg atagaggtta tagtataact caaaaaaatg
    16801 gaaaagtaat aaatagtata aaaggattaa aaataaagga tagtatagat atagtattaa
    16861 aagatggaaa tttagaatgt atgatagata aaattgaaaa taaagaggga tagtatgaat
    16921 ttaacctacg aagaagctta taaaagatta gaaagtattt taagtgaatt agaatctaaa
    16981 aatgctagct tggatgaatc tttaagccta tatgaagaag gtataagcct ttataaacat
    17041 tgtaataagc ttttagatga tgctaaattg aaaataagta aatttaatca attaggaata
    17101 gaagaagact ttaaaataga ggaggaataa aattggaatt taaacaatgt ctaaaagaaa
    17161 aagcaagttt tgtagaaaaa gtacttaaag aatatatgcc taaagaagaa gggtatcaaa
    17221 aaactgttat tgaagctatg aactatagtt taagtgctgg aggtaaaaga ttaagaccaa
    17281 tacttacatt ggaggcttgt aaaatagttg gaggaaatga agatgaagct attccttttg
    17341 ctattgcaat tgaaatgata catacatatt cactaataca tgatgatttg ccagctcttg
    17401 acaatgatga tttaagaaga ggaagaccaa caaatcataa agtatatgga gaagctatgg
    17461 ggattttagc aggagatgct ttgttaaact atgcttttga agtaatgctt gcaggctcaa
    17521 tcaataaaga aaatccagaa aagtatctaa aagctataaa tgaaattgcc aaaggtgctg
    17581 gtatatatgg aatgataggt ggtcaagtag tagatgtaga aagcgaaaat aaacaaattg
    17641 aaaaagaaaa attagattat atacatatga ataaaactgc tgcaatgatg gtaggctgta
    17701 tgagagcagg agctactata ggtggagcaa attctgaaca aatggaagaa ataactaaat
    17761 atgcaaaaaa tatcggatta tcattccaga tagtagatga tatactagac atagtaggag
    17821 atgaagctaa gttaggtaaa aaggttggaa gtgatataga aaatcataag tcaacatacc
    17881 catctctttt aggtttagat aaatctaaag aaattgcaca taatttgatt gatgaagcaa
    17941 agaaaagtat agaaaaatta tctgatgatg tagatttttt aaagggattg gctgaatata
    18001 taatagatag agaatactaa aatatacagc taaaatcgag gagggaaaaa tggatttttt
    18061 ttcggagatt tttaacaatg gggctttggg aataagtctg attgcttgtt tcttagcaca
    18121 atttataaaa atatttactg gtaaggaaaa aagaattgag ttatcaagaa tacttatttc
    18181 aggaggtatg cctagttccc atagttcttt tgttacaagt ttagctacag tggtaggtat
    18241 tgaaaaaggg tttaattcta ctgattttgc tataattacc gtacttgcct taattataat
    18301 gtatgatgca gcaggtgtta gacgagctgt tggtaaacaa gctactatat taaatcaaat
    18361 ggtggcagat atacagcatg gaaagcatat agagcagaag aaattaaaag aattaatagg
    18421 tcatacacca ctagaagttt ggtttggagc tttacttgga atagtaacag ctttaatatt
    18481 gatgtaaatt gaaaatttac agaataaagg tgaggataat atgtataaat atttagataa
    18541 agtaaattct ccaaaggata taaaaaatat gagtatagaa gaaatggatt tacttgcaaa
    18601 ggatataaga aagttcttag taaaatctgt ttcaaagaca ggaggacact tagcttctaa
    18661 tctaggagtg gttgagttaa cattagcact tcataaagta tttgatagcc ctaaagataa
    18721 gattgtatgg gatgtagggc atcaatctta tgttcataaa atagttactg gaagaaaaga
    18781 ttgttttgta tctttgagac agtttaatgg actaagtggt tttccaaagg agaatgaaag
    18841 tcctcatgat atttttgata caggacatag cagtacatct atttctatag ctacaggtat
    18901 tgcctgcgct agggatataa aaaaagaaaa ctatagtgtt atatctgtga taggagatgg
    18961 ttctataaca ggaggtatgg cacttgaagc tttaaatcaa ttaggatata tagataccaa
    19021 tatgatagta atccttaatg ataatgagat gtccatagat aaaaatgttg gaggaatgtc
    19081 taaatatctg tcaagtatta taagaaactc aacagttgag aaaatgacag atgaagttga
    19141 taaaatttta aatgttacac aaacaggtga aattttatct aagactgcac atagatttaa
    19201 agataaacta atgtatagct tttctcctca ggactgttca ttttttgatt cattaggtat
    19261 aagatattat ggacctatag atggccataa tactaaagaa ctaatagata tattgagaaa
    19321 agctaaacac aaaaaagggc ctgttctttt acatgtaatt acgaaaaaag gaaaagggta
    19381 cagatttgca gaagaacaac ctgataaata tcatggagta tcaaagtttg atataaagac
    19441 aggtgttaca tctgcaaaag ttaagtctat gtcaattagt gttggagaaa aacttgttga
    19501 tatggctaat aataatgaag atatagtagc aataacagct gcaatgccat ctggtacagg
    19561 gttaaacctg tttgaaagtg catatccaaa aagatattat gatgttggta tagctgaaca
    19621 acatgccaca ggatttgcag caggtcttgc aaaaaatggt atgaaacctt actttgctgt
    19681 gtactcatca tttttgcaaa gggcatatga ccaagttatt catgatgtat gcataacaaa
    19741 aaagccagtt acttttctta tagatagagc tggattagtt ggaaatgatg gagaaactca
    19801 tcatggtatg tttgatttaa gttacttaaa ttctattcca aatattgtag taatggctcc
    19861 aaaagacaca agagagatgg agcttatgat ggatttgtca ttaaaattag attgtccact
    19921 agcaattaga tacccaagag gaagtagtta ttacctagat aagggagaat atggagagat
    19981 tgtactgggt aaatatgagg tattagatga cggtcaagat acagtcatac tttgtattgg
    20041 aagtatggta aaacatgctt tagaggctaa agaaatatta tcaagagaag gaataaatcc
    20101 gacaatagtt aatgctagat tcttaaagcc aattgatgaa ggtatgttaa aagcattatt
    20161 aaaaaatcat aaaaatgtag ttactataga ggataatatt gtaactggag gatttggaag
    20221 tagaataaac aagtttatta tagataatga atataacgta aatatactta atatagcaat
    20281 accagaagaa tttgtaaaac atggcaatat agatgaatta tatgattttg taggattatc
    20341 gccaaaaagt attgctgata aaataaggaa attagttatt gagtaaaaat ctaatgtaga
    20401 tattgtaaat taaaatttat ataaaatata gaatttatca attagaataa atatcaagtg
    20461 tgttacttca agctacttta gttatcatta aagtagcttt taacagaaag gtaattgtat
    20521 gaaaaaaaga atagatttat tattagtaga acaagggtac tttgaaagta gagaaagagc
    20581 caaaaaagca ataatggctg gattggtttt tgttgataat caaaggtgtg ataaggctgg
    20641 tacagaagta aaagaagatt gtagtataga agtaaaagga aatccaatac catatgtcag
    20701 tagaggtggt ttaaagcttg aaaaagcaat gaaaaacttt gatttgacta ttgatggaaa
    20761 ggtatgtatg gatatagggg catccacagg tggttttaca gattgtatgc tcaaaaatgg
    20821 agcaataaaa gtattttcta tagatgttgg ttatggtcaa cttgcatgga aattgagaca
    20881 agatgatagg gttgtgtgta tggaaagaac taatataaga aatgttacaa tagaatatac
    20941 aaagcagttt gctgattttg cgtcaattga tgtgtctttt atatcgttaa agttagtact
    21001 tccaaaggca aaggagttag tacgtcatga tggggaagtt gtcgctttaa ttaaacctca
    21061 atttgaagca ggaagagaaa aagtaggtaa aaaaggagta gttagagaaa aatctacaca
    21121 tatagaagtt ataaaaatga tttctgattt ctcagtcgaa aatggatttg agattttagg
    21181 gttagatttt tctcctatta aaggtcctga aggtaatata gaatatctaa ttcatttgag
    21241 aaatggaaat gaaggatatg aatttgatgg tgagacatac aataacaaaa tagtagaagt
    21301 tgttgaagct tctcataatt tagacaaata ggttaaaagg gggcttagat ttgatcctcg
    21361 aattatatat gaaaaattgt gccttagttg aagaactaag gttgaatatt gataaaaact
    21421 taaatatact tacaggtgaa actggttcag gaaaatctat aataatagat gcacttggac
    21481 tttgtttagg tgataaatat gataggtctt ttttaagaaa aggaacagat aagggacttg
    21541 ttgaggctgt atttttttca gacaatagat atcttaaaaa aatattagaa gaaaatgata
    21601 taagtatgga agatgataat ttattggtaa taactaggtt gatatactca gatggtaaga
    21661 gtactgctag agtaaatgga aggactgtta aagtgtctct tcttaaagaa attgcatcta
    21721 cacttataga tatacatggt caacatcaaa atcaagcttt gttcaataaa gatactcatt
    21781 taaaatttct agaccttttt ggagaaaatg aattggaaga gtttaaaatt gcttataaaa
    21841 atgtgtacca taagtatagt gaagtaaaaa aagctttaaa ttgcttgaca gaaaataaag
    21901 atgagatgca aattcaacga gaaatagatt tattaagatt tcaaataaat gaaattgaag
    21961 ctgcaaatct aaataagaat gaatatgaag atttattaaa acaaagagaa gtttatagaa
    22021 atagtgaaaa gatatataat aatctaaatt cgagttatag caagttacat aatggtgagt
    22081 ataatgttat tgacctaata ggtcttgcat ctaaggaact taatgacata tctaaatatg
    22141 acagtgtttt aagtgaatat agtgatactg tagaaagaat tatgtatgag ttacaagaca
    22201 tatctggtga gataagaaat tacaaagata atatagactt tgaaccttat gagctagagc
    22261 agattgagct tagaatcgac gaaattaata atttaagaag aaaatatgga gacagtatag
    22321 atgatatatt tgagtattat ggaaaaatta aagatagatt agatgaaata ttaaatagag
    22381 atgaaagagt tgaacagtta aaaagcaaac tgatgaatat agaagaagat ttaaaaataa
    22441 aagccagtaa attgactaaa gcaagaatag aagtagcaac tactttagaa aaaatacttc
    22501 ttgatgaatt aaaaagctta aatatgaaaa atgtaatgtt caaggttaat tttgaaaaag
    22561 gttcatttac tttagatgga gtagatgata ttgaatttat gatatctttt aacttaggag
    22621 aagatattaa gcctatatat aaagttgcat caggtggtga gatgtccaga tttatgttag
    22681 cgtttaaaac catattggct gatatagatg atattgatac attagtgttt gatgagatag
    22741 atactggaat aagtgggata gcagcacaga tagttggtga gaaattgagt gatatagcaa
    22801 agaaaaaaca gataatatgt ataactcatt tacctcaaat tgctgcaaat gcagacactc
    22861 attattgtat agagaaagat acttcaaata ataggacgtt taccaatgtt agtaagttaa
    22921 atgaatctca acgaaaaaat gaaattgcca gacttatagc aggtaacaat ataacagaaa
    22981 aaactattga acatgcaagt gaaatcattg aattggctaa aaaatgttag tgaaaaacaa
    23041 gcattttaat aggtgtaaag aatttttata gaaatgcttt acacctattt tttatgatga
    23101 aattatttta aaatatctag tgcctatatt gaagaaaagt aggtttgaaa atggaatgaa
    23161 ctttgtataa atttgtaact ttgaaatttc taaatgctta agtataatat tatttattaa
    23221 ggtagtagtg aggggaggca taaaatgaat aaaaaagata ctatgcactt aatttttata
    23281 attgtaattt ttattttatt atggtatatt gtggtagcag taagtgagat taataaaaat
    23341 attgaaagtt gtagtaacaa attagattat ttgtcacaag agcttatgag tactaagtcg
    23401 gatattagta atactataaa tgaagaaatg tcaaaaagtt atataacaaa aagtattgac
    23461 ttaaaagtaa aaaaaatcga gaaaaaagaa tgtactattg atgtagatgt tcaattaagt
    23521 aaacttggaa aaaatggaaa agtatttttt atgtataaag aagatagtga taaatggaat
    23581 gaaactgaga tgacaaaaca tggggaatta tcatatgcat gtgaaataaa gataaataca
    23641 ggaagtgaat atgattataa agtagttaca agtggagata tatctgaaag ttctgatgta
    23701 caaaaactga ctaaatctga ttatatgcct gaacaaccta tatttaattc tgggataaga
    23761 gataatagag aatactttat tgaaatagta gaaaatagta atctttttaa tcatgagagt
    23821 gaaaagtcaa aaaataaagc ttacgataat atcaggttag aaaaagtaga tattattgta
    23881 aatgaaggaa aaaaagacac tatatacaaa gcccaattag ctgaaggctt agatgatggc
    23941 aaaactaata attcaaatag aaatcaagtg aattatgaag taagttttcc taaaagggat
    24001 attgatgaca taatttatat taaagctaaa cttacataca ataatggagt agaatataca
    24061 aaggatataa ctgaagatat aactatggga ttacaagatt tagaaaaata aatacatatt
    24121 taaataaata ttcaaataga tttgttcaca aatagagtat aaataaaact gtagtagttt
    24181 tacttatact ctattaaaaa attataattt taattccata tatctagccc cttcaattgg
    24241 attataagta taaagttcta tatcataaaa accaaacttt tcatataatc cttgtgcact
    24301 tttcatgctt ggaagagtat ctaatctcat gtgtgtatat ccaatttttt tagcttctac
    24361 aatgatttct tccaatagta tttttcctat ttttaatcct ctaaattgat ttcttacata
    24421 taatcttttt aattcacata ttttaccttc aagttttttt aaagcaacac aacctgccaa
    24481 attttcatca acaaatgcca atatcaatga gcctgaaggt tttttatatt ttcctggaag
    24541 tgtttttaat tcattattaa agtcttgaaa acataagtct atatttagag aatttgaata
    24601 ttcagtaaat agaagtttta cgttttcaag attttctaaa tctttttctt caacaaattg
    24661 taaagttata ttttgagcca ttttatccgc cattatataa acatgtttat ataatgactt
    24721 cacctccttg atatatgtat atttttctta atatcaatta gattttaata ccattacttt
    24781 ttcaccacca tagtcaagct ctccattaaa cttaaatcca aaatctttat aaagcttaat
    24841 agccatttta ttgtcttcga atatactaag atatagttca tcacagttat attcattttt
    24901 aagatgattt acaagaaaat ctaatgatgc ttttccacat cctttacctt gatattttga
    24961 atcaatcata aatctatcaa gccaaactct atcatcttct aaacgaccat acatagcaaa
    25021 tcctattaaa gaattttcat catatattcc aacaggtacc caagctgtta taaatttaga
    25081 ttcagcaata gaaagtgcat tgctttctat gtaatctaac tgacttttat ctacagacaa
    25141 actaactaaa tcgtgccaat tatttatgtc tgcaattctt acatttaatt tcaagatatt
    25201 accgtccttc aaactttttg t
//
LOCUS       DACTGW010000039        24490 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.039, whole genome shotgun sequence.
ACCESSION   DACTGW010000039 DACTGW010000000
VERSION     DACTGW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.039"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            420..2513
                     /locus_tag="I9573_001231"
     CDS             420..2513
                     /locus_tag="I9573_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT4795858.1"
                     /translation="MNKRNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEQNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTILNKPIKINEFNEISNTKEGKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTQSHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
     repeat_region   3174..3467
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3174..3202
                     /rpt_unit_seq="gttttatattaactatatggaatgtaaat"
     gene            complement(3635..3919)
                     /locus_tag="I9573_001232"
     CDS             complement(3635..3919)
                     /locus_tag="I9573_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795859.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     gene            4126..4944
                     /locus_tag="I9573_001233"
     CDS             4126..4944
                     /locus_tag="I9573_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT4795860.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            complement(5077..7197)
                     /gene="feoB"
                     /locus_tag="I9573_001234"
     CDS             complement(5077..7197)
                     /gene="feoB"
                     /locus_tag="I9573_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAT4795861.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(7223..7450)
                     /locus_tag="I9573_001235"
     CDS             complement(7223..7450)
                     /locus_tag="I9573_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAT4795862.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(7777..8217)
                     /locus_tag="I9573_001236"
     CDS             complement(7777..8217)
                     /locus_tag="I9573_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4795863.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     INLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            8369..9358
                     /locus_tag="I9573_001237"
     CDS             8369..9358
                     /locus_tag="I9573_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HAT4795864.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            9834..11042
                     /locus_tag="I9573_001238"
     CDS             9834..11042
                     /locus_tag="I9573_001238"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAT4795865.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     gene            complement(11103..11984)
                     /locus_tag="I9573_001239"
     CDS             complement(11103..11984)
                     /locus_tag="I9573_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HAT4795866.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            12370..13314
                     /locus_tag="I9573_001240"
     CDS             12370..13314
                     /locus_tag="I9573_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAT4795867.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLI
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            13537..14082
                     /locus_tag="I9573_001241"
     CDS             13537..14082
                     /locus_tag="I9573_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HAT4795868.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            14173..15321
                     /locus_tag="I9573_001242"
     CDS             14173..15321
                     /locus_tag="I9573_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HAT4795869.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQNSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSKKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            15374..16639
                     /locus_tag="I9573_001243"
     CDS             15374..16639
                     /locus_tag="I9573_001243"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAT4795870.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(16925..18157)
                     /locus_tag="I9573_001244"
     CDS             complement(16925..18157)
                     /locus_tag="I9573_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4795871.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(18160..18834)
                     /locus_tag="I9573_001245"
     CDS             complement(18160..18834)
                     /locus_tag="I9573_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4795872.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLVN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            complement(18842..20071)
                     /locus_tag="I9573_001246"
     CDS             complement(18842..20071)
                     /locus_tag="I9573_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAT4795873.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            complement(20183..21187)
                     /locus_tag="I9573_001247"
     CDS             complement(20183..21187)
                     /locus_tag="I9573_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4795874.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLINEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            complement(21211..21885)
                     /locus_tag="I9573_001248"
     CDS             complement(21211..21885)
                     /locus_tag="I9573_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4795875.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            22173..22940
                     /locus_tag="I9573_001249"
     CDS             22173..22940
                     /locus_tag="I9573_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4795876.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALKGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTMDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            22927..>24490
                     /locus_tag="I9573_001250"
     CDS             22927..>24490
                     /locus_tag="I9573_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4795877.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVKTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKMFFKDFEIEKINLYIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKFKNIVSENMKFTYP
                     FNFTSSIESYNNL"
BASE COUNT         9153 a         2969 c         3664 g         8704 t
ORIGIN      
        1 ataagttttt tgttttacta tatatattaa aagtgattaa gtctattaga aaaattatga
       61 tgtttttaga aattaatgct ttttaacttc aatagtatca ataatactga ttttttataa
      121 aaatttaaaa caataataaa atatataatt tatttttgtg gacaatttgt caagattagt
      181 accaagttaa aatttaatat aagagagaga tgtattatgt aatattaatt ttttttaaat
      241 tggtaatact atattgctgt attttttttt atcaattata atttaaaata tgaaataata
      301 aactttctaa ttaaattaag gtatttagtg tgtatgtata agttataagt aaatcttcta
      361 aaaagaataa ttttaacagt gttgttataa agagcagtaa ctatggagga gataagtata
      421 tgaacaaacg taattttgaa gttatattaa atcaactaca gattaatata tatgttacaa
      481 atattcatac taatgaaatt atttttatga acaagaaaat gaaagaagaa tataatattt
      541 tagatccaga aggaaaagtt tgctggcaag tattatatcc tgagcaaaat tctacttgta
      601 gtttttgtaa agttttagaa ctattaaaga atgataaaaa aggagtatta ataaagtggt
      661 atgaaaaatg taataagtta aatcgtgttt ttgaaaatta tgatagttta attacatggc
      721 aagatggcac agttgttcat atgcatcaat caatagatat tgcaaactca acaatcttaa
      781 ataaaccaat taagattaat gaatttaatg aaatatcaaa tactaaagaa ggaaaaggag
      841 tttttaattt ttcaagagac aattttgatt ataattctac attgctttat gatgctttga
      901 ttagaggtac agatgaatat atatacattt gtaatatgaa aacaggcgta tttcgatatt
      961 caccatcaca agttgaatta tttgacttac caggagaaat tgttgaaaat ccgcttattt
     1021 actggaaaaa aattgtacat ccagaagact ggaatcgttt ttataagtca aacactgaaa
     1081 taggtaaaaa ccagatggat tatcatacag tggaatttag ggctaaaaat cgtagtggag
     1141 aatatatatg gttaagatgt agaggacaac taatgcgtga tgaatttgga gaaccaagta
     1201 tttttgcagg aataatgacg caattaggta aacaaaataa gattgattca ttgacacagt
     1261 tactgaatta tcatgaattt atgagtgtat ttgaagataa aatttcaaat cctatgattg
     1321 aaaaattatg catagtatta ttaaatattg acgattttaa aaatgttaat gagatgtatg
     1381 atagggattt tggagataat ataataaaaa cactagcaca gtctatacaa tctattttac
     1441 cagataatgc tgaactttat aaacttgatg gggacgaaat gggtattctt gttgataatg
     1501 ttgaagaaaa tgaaattcta actctatata atcaaataca gaatatgatt atacaccttc
     1561 aactatggag aaaatatgga ttaaatataa caatttctgc tggttgtgta atatatccaa
     1621 aacatggaga tacagtaaaa gaactttata aatgcgctag ttactcttta caatatgcga
     1681 aagagcatgg taaaaacagg cttgtttttt tctcccaaga aatattaaaa aataaaatgt
     1741 attcattaga aatgatgcgg gatttaaagg catcaattaa tgatgatttt aggggattta
     1801 gtcttcgatt ccagccacaa gtagatacac aaagtcataa aataataggt gtagaagttc
     1861 ttttaaggtg gactaatgac aaatgcaaag caatttctcc tttggaattt attccaatat
     1921 tagaagaaaa tgatatgata aatattgtag gggcttgggt gcttcgaatg gcattacgta
     1981 cttttaggaa atggattgat tactatcctt tttttaaagt gagtgtaaac gtttcagctg
     2041 ttcaaatatt agaagacaca tttattgaag atatagtaaa aattatagat gatgaaaatt
     2101 ttccttatca aaatcttgtt ttagaattga cagaaagtca tacagtacaa aatatgagta
     2161 ttttacagtt taaatttaag gctttacaag atttaggaat ttatattgct atggatgatt
     2221 ttggtactgg atactcgtct ctagaagttt taaaattttc tccaatagat atagtgaaaa
     2281 ttgatagagt atttgttaaa gatatattaa agagtaagtt tgatgctaca tttatacatt
     2341 ttattgttgc aatttgtcat gatgtaggaa taaaagtatg tttagaaggt gtagaaacac
     2401 aagaagagta tgatttagtt aaacaaataa agccagatta catacaagga tatttatttg
     2461 gaaaaccgca gacagcaaca gaaatctttg accttttaaa gctagataat taattctaat
     2521 ttatcaataa tatcactgaa aagctttaaa atcaaaaata cattaaatta ttatattgaa
     2581 gatatatcaa ttataaattg caatataaga aaatagcatt tcaagggatg ttaaattagg
     2641 agtagtatat gattaaagta ttttaaaaag ggataactat aataaaaata gatatctatt
     2701 tttagattaa aaataatata tcataaataa aataataaga ggtagataca gttttaaggg
     2761 aatacaaaag tttttaatta aactatgctt gttcagatag atatttattt aagaaaaaag
     2821 actattaaaa gcaatataca agaatgatat attagattga ttaaacaagc ataaatatta
     2881 tgtaaaaaac tttaagttat agaatttaaa tccaatgtag atagattacg ttttttcttg
     2941 cttttattat ggtataaatt ggtatcaata ttcaaaagta atatatttat gatataataa
     3001 aattatagaa attttgcagt gagctatatt tgtgataaaa tttggcgtaa cagttgaaat
     3061 ataaggtgtt gagagtgcat gataagcgtt atcaattgca ctattgctcg ttcactgcaa
     3121 atttaggaga gttgtatacg tgtaagtgtt gaaaatacta agtttatttt ggggttttat
     3181 attaactata tggaatgtaa atctcggaga atttcctaat gtggatttaa taggaagtgt
     3241 tttatattaa ctatatggaa tgtaaatttt agcaaatttt tcgtaaaagt cactattttc
     3301 aatggtttta tattaactat atggaatgta aatacatata aaactttcat cttctgttaa
     3361 ttcattaaaa cgttttatat taactatatg gaatgtaaat ccatcttttg ttgttttaca
     3421 taaatttata ttacttacgt tttatactaa ctatacggaa tataaatttt tataattttc
     3481 ttttaatgtg ttatcttatg attatctagt tatattagca tacaaaaata taataaaatt
     3541 acactatatt ataaaaacca aaaggtagta tagaaatcct attacctttt tattattaat
     3601 tttatcagtg ttttattata aatagcttac ataattacac attttttctg attaaataat
     3661 atgatgctat tgttataata gatactaatg ctaatgatgt tattgcagta tctaatcttc
     3721 ttattaataa actcatttct atatattcaa gttctattaa atattgataa aatatttcta
     3781 gtttaacagc tttttctcta tctatatttc catactttaa ctctaattta tctaaagatt
     3841 ttttattaca ataattaact ttatgtttaa ttaaatatct tattgtagca cctattacaa
     3901 ttttcactct aacaagcata aatataatat tccatccaaa agttaagagg ggatatcttt
     3961 tttatgagag gaatggataa aatatttata actgacatct aactgatatt taagctttaa
     4021 aatcaaaacc atattgattt tctaaaataa taactacttg atttagtaaa taataaagaa
     4081 tatagtaact atatagacaa caaaaagaaa aggagtgtat aacccttgga aaaacagtat
     4141 tttacaacag gtgaatttgc taaattgtgt ggtatatcta agcagacatt aattttctat
     4201 gacaaaatag gtattttttc tcctgaatat aaagataaaa ataattatag atattactca
     4261 gtttatcaat atgatacact tgatatatta caatcattaa gagaaatagg aatgagttta
     4321 gaggaaataa aagagtatat acaaaataga actccccagc tttgtgtgaa aatgcttaaa
     4381 gaagaagaaa aaaagataag agaaaaaata aaaaaactaa gaaagataag ctctaagatg
     4441 caaaatagaa taaatataac agttgaagga atttctaaaa tgaatagtga agaaatttat
     4501 atatccaaaa agtttgaaga atatttagtt ctatcaagcg atttgagtaa tatgagtaat
     4561 agtgatttta tgatggaatt aatagatttc accaattatt gtaaatcgaa aaatttgtat
     4621 caaggatatg agttgggagt aatagtaagt aatgaaaata taaaaaatgg ggactattta
     4681 agtatatcaa gtttttattt gaagatagat aaaaaaataa aggataaaaa gctatatgta
     4741 aagccagaag gaatgtatgc ttgcataaaa catattggaa aatatgagga ttcatataaa
     4801 tcttacgaaa aattgaaaaa atatatatat gaaaatgaat ataaaattat tggaaattcg
     4861 tatgaggaga gtatacttga ctttttttgt gaaagtaatg aagataatta tatgactgaa
     4921 atttcaattc aagtatccag ataagaatta aaatagagat atattcaaat gttaagtaat
     4981 tttacttatc attgaatata tctctatgct ttataataaa gtatataaat ttttaaatag
     5041 tttctctaat atatgtctaa tgtcagttaa tactaattat ataaataaat tgtaaactgt
     5101 agttgttaaa aaacaaacaa caaaagcaag tatagtaggc attaagaatc ctaaggcagt
     5161 ccattttaaa ctaccagttt ctttttttat tgttaaaagt gttgttgcac aaggccaatg
     5221 cagtaaactg aacagcataa catttaaagc tgtcaaataa gtccatccat gttctctaag
     5281 aacttgacct aaagcactaa agctatctaa ttctatcata gaaccagttg ctaggtaagc
     5341 cattaaaagt ataggtacaa ctatctcatt tgctgggaat ccaagaataa aagcaagtaa
     5401 tataaaacca tcaagaccaa ttaatttagc taaagggtct aaaaagtttg caacatgtga
     5461 taatatgctt aaatctccta tatatatatt tgcaaatatc caagttatga caccagcagg
     5521 tatggctacc attacagccc ttcctaaaac aaatattgtt ctatctatta tagaggtata
     5581 taaagttctt cctatttgag gaactctata aggaggaagt tctaatgtaa atgtagacgg
     5641 aactcctttt aatagcgtct tagaaagagt ataagaaacc aacaatgtta ttataacacc
     5701 tagaattatc agtaaagtta tgcaaagtgc agttgctacg cttgatacga aagagttggt
     5761 tataactgag ctaaaaaata ttgtagatat agctatcaaa gtaggaaaac gaccattaca
     5821 aggaacaaag ttgtttgtaa gtatagctat aagtctttcc cttggagagt caattatcct
     5881 acaaccaata actccagctg cattacatcc aaatcccata cacatagtca aacactgttt
     5941 tccatgtgca caagcttttt taaataggtg gtctaagtta aatgcaactc ttggcaaata
     6001 tccaaaatcc tcaagtaggg taaataatgg aaaaaatatt gccataggag gtagcattac
     6061 agagattacc catgctaatg ttctgtataa tcctaaaacc aacatatcat ttaaccatga
     6121 tggacaatta atactattta atatacccga aatacttggc tcaaatccga gtaataaatt
     6181 agataataaa gtggatggat agttagcccc ttctatagta atccacaata tagtacctaa
     6241 taataagagc attattggaa gcccaaatat tttagatgtt aatattttat ctactttttc
     6301 ttctctatca gttgattttt tagcaacatt agaacaacac tcatcactca gttttttagc
     6361 ataatcatag ttgattttag taaactcatc tcttattttc tgtttattta tattgtctgg
     6421 aatctttttc tttacttcat taatagcatc tatagaatct ttgtctatat aattactcat
     6481 agattcaaat atactttcat cgccatcaat aagtctaagt cccaaccatc tagaatttat
     6541 acctggtata atattatcca attctggttg aattgatttt accacattct ctatattttc
     6601 attgtatcta acaggtttat tatttaattt gtatttgtca aaagatacat catttaaagt
     6661 atccaatagt tcatccatac cagagccatt tcttgcagca gttaaaataa ctggaatacc
     6721 aagagaatcc tctaaaagtt ttttatcaat agtaatgcct ttttttctag cttcatctat
     6781 taaattaata cacaaaataa ctttatctgt aagttccatc acttgaaata ctaaatttaa
     6841 atttctttca agacatgtag catcacatac tactattact gcatctggat taccaaaaca
     6901 tataaaatca cgagctacaa tttcttcttg tgataaggca aataaagaat atgttcctgg
     6961 taaatctatt aatgcatatt cggtattttt atatttaaaa ttaccacgag ctgtagctac
     7021 agtttttcct ggccagtttc cagtatgctg tctaagccct gtaagatgat taaatacagt
     7081 acttttacct gtatttggat tgccagccaa tgcaattaca aactggtctt ccttattgtc
     7141 tatatcaaac atatctttta aagaactcat tttagttgag ttatgtgtca accccatatt
     7201 aatatcctcc tgctagaaat ttttaaatgt cagaaactaa tattaagcta ctttcttctt
     7261 gtcgtagagc tatcttacta cctctgattt tatagacagt taaattattt tttggacctt
     7321 ttctaactac atctataaca gctcctctag taagaccaag cgctaacatt ctttctctta
     7381 aattaccact agataatata tcttcaactt tgacagtttt tttcacttga atatcgttaa
     7441 ggtttttcat agtagcacct ccatgcttga aatttagcct gggctaatct tcttattcta
     7501 aactatgcat tatattatta ccttgttact tttacaaaaa aaagataggc aagatgccta
     7561 tcatacaaag ttgggtataa ttcttacttt tggggaaagt agaatataga ataaaaatta
     7621 acactttgaa tccatataat tattgaattt tgataataaa aaagtcagaa agcaaaagcc
     7681 tcctgactct atgtaaggta ttttcaaata cctaaatatc aattcaatat ttaatttata
     7741 cttaaattat atattaacaa tattagcttg tcaacactat aatctcattt taatatgttc
     7801 aatacctgct tcgtcgtata cttctgagat ttctttaaaa ccacaagata agtaaaggtt
     7861 ctttatataa gtttgtgctg ataaggttat attattttcg tgtaagttaa tttttataaa
     7921 atctatagca caatccatca tttgttttgc taatcctttt cttctatgac tagaaagaac
     7981 taaaactcta ccaatggaaa catcattata agagctgtat tctttaggaa ttattctagc
     8041 ataagcacaa atcaatccat tttcttttaa aaatatgtga taagagcttt tatctatatc
     8101 atcataatcg tttaatgaaa atatcttctg ttcacaagca aatacctcat atcttgattt
     8161 tgctatttca taaaattcat ctaaactcaa atcattaaag tgtttaattt taaacatagt
     8221 aattacctcc tagcatctta aaaaactgaa tattaagata cattatacca tttttaacat
     8281 aaataccttt attaaaattt atatttatta aaaatatttt taaaaagtga tatactatat
     8341 taaaaacaaa actttagtta gaaaatttat gatttatgga ggcagtgttt tgaatactac
     8401 taaaaaatta actgaagcag ctttattgtc atctttattc atagtagtaa caattatagc
     8461 tgtgagtact ggatttggat atgctattta cttagatttt atagtgccag tatttttttg
     8521 tataatctgc ttgaaatgtg atttaaagta taccatttta tctggaatta cttcactttt
     8581 aataatttct ctggtacttg gaaatctagg aactgctatt tgggcaagtc agagtgtatt
     8641 acttggtata atgtgtggct atctaataaa taagcctaca atggttatgg atgatttagt
     8701 atatggttca gtatttgggg ttattgtaat ggttttcatt gatatatatg cttcaacatt
     8761 aataggttat agttttatga aagaatttca aggatatagc aaatggattt attttaatgg
     8821 gtatacaaat tttatttatt atttaatgat agcattgttt ccttttggta tggtgttttg
     8881 catatattta ttaagtttaa ttttagggaa taaattgcat attttagatt ctaattcttt
     8941 aaaaaaattt ttaatattta agaattttag aagtttaaac caatttttat gttgctcaaa
     9001 aaaagctttt tacatatgtg tttcttatct agccattttt gaagttctaa aactgttgaa
     9061 tgttaaagtt gattctgttt atttaaaaac aatctttata tcaataacct atataagtgt
     9121 ctattttatc attagagact catgtatgtc actacaaaac tttataatag ctaagtttag
     9181 aaaattatta tatgctagaa ttttattttt gataataatt ttattgctgt tttttatatt
     9241 tgatgtaaca gttattagtt taataattat aaacgcattt ttaaataaga aaataaatat
     9301 aagattgagt cagagtaata tagtcaataa acaaataaat cttttgattg aaaaataaac
     9361 tttgggtatt ggtaaaaaat ataaaaaagt agtataatgt aaaaaaacat gtatataata
     9421 ataaaaaagg ttgacaaaat ataaaaataa taatatatta tgagtaagtt aagaaaaatt
     9481 tcttgtaatt gatgaaataa tctgaatata taaatgctat gaagagaaga agtagggcta
     9541 gattttattt aacagagagt tccagtttgg tgaaatggaa taaattgatt tagtttgaat
     9601 acatctcaga gcactatctc tgaaagataa caaccattaa gagatatcgg attagtacac
     9661 gttatagtac acgagtatat gtaattgtac tctaagagat taatatcgtg agatattgat
     9721 gaactaaggt ggtaacacgt aagcaatgct ttcgtccttt taagaggatg aaagcttttt
     9781 ttattataaa aaaaaaatta tagattatat aaatatttta ggaggaatga aaaatgaaat
     9841 cgatagacga gcaaatgcga ataattatga aaggtgtaga tgatttaata gatgagaaag
     9901 agttaaggga aaagcttatt aaatcagaaa aagaaggaaa gcctatgata gtaaaattgg
     9961 gtctagaccc aagtgctcca gatatacatt tagggcatac agtagttctt agaaagatga
    10021 aacaattaca agatttaggt catcaaatag taataataat tggagatttt acaggaaaaa
    10081 ttggagaccc aactggaaaa tctaaagcta gaaaagcttt aacaacagag caagttcttg
    10141 caaatgcaaa aacttatgaa gaacaaattt tcaaagtttt agataaagaa aaaactattg
    10201 taagatttaa tagcgagtgg ttagccaagt taaattttga agatgtaata aaattagcag
    10261 caactattac tgttgcgaga atgttagaaa gagaagattt taaaaagaga tatgaaggac
    10321 aaatgccaat atcagtacat gagttcttct acccattaat gcaagcatat gactctatag
    10381 cattagaagc tgatatagaa cttggtggaa ctgaccaaag attcaattta ctaatgggaa
    10441 gatcattaca aagagaattt ggtatggagt ctcaaatagt aataatgatg cctttaatag
    10501 aaggattaga tggtaaagaa aaaatgagta agagtttagg taactatatt ggaatagatg
    10561 aagaagcggg aataatgtac caaaaatcta tggaaattcc tgatgaactt ataattaaat
    10621 actacaattt agtgacagat gttcatcctg atgaagttaa taaaatagaa tctcagttga
    10681 aagagggttc agtaaatcca agagatataa agatgaactt agcaagagaa atagtaacat
    10741 tataccatgg agaagaatct gctaaagaag ctgaagaaag atttaaatct gtattccaaa
    10801 aaggtcaaat acctgaagat atacaaacaa ttcaagttaa ggaagatgga tttgacttaa
    10861 tcgaagtact agtttctaat gagattgtta agagtaagag tgaagttaga agattagctt
    10921 ctcaaggtgg agtaaaggta aatggtgaaa aagtagagga tttaagcact attgtaaaag
    10981 agagtgaatt agttgttcaa ataggaaaga aaaaatttgt aaaaatcgag ctagtaaaat
    11041 agagtaaagt aaaagcaagt ctattgagta taaaatgtac attcaataaa cttgcttttt
    11101 tattatatta aaattttaaa ttgataacca ttgtctttaa aatatgttat aatttggtcc
    11161 aatgcattta ctgtttcttg ttttccataa gtatcatgca ttaaaagaac aaccatttct
    11221 ttgccttcag aagatttctt tgcatgttca aataattctt gtgcattttt ctttttacct
    11281 tctgcatcag catttaaagc gttccaatca attgatgcca tgtttttctc ttttaagtag
    11341 tttcctagag gttccatatt tttccatgac atatacccgc ctgggcatct aactacattt
    11401 gaggaaaaat tctttcctaa gacttttttc atagcttcat cagttttgtt aagttcattt
    11461 acgaatgtat cgagatttag gcttctgcca ggatatagtt ttttatagtc atgggtataa
    11521 gagtgatggg ctattgcatt tccttcgtca aatgttcttt ttaaaatttc attagctcct
    11581 tttctttcaa gagaatcacc cttaataaag aaagttcctc taacaccatg tcttttcaag
    11641 atatctagaa cttgtggagt attagtagta gatggtccat catcaaatgt aagaaagact
    11701 atcttttctc cattgtttga atagtcgtat ttgttaagtt tgtcccaaac tatttttgcg
    11761 tcatatccat attcttttac agcttctttt tttgcaccaa taataggttc atttttttct
    11821 ttttcaattc tttctttttc taatctttca gctttaagtc tttcttgagt ttctttcatt
    11881 tctttttttc tagacatgtc cgcccatgcc atagaaaaaa taccaataac tattacaaca
    11941 agtatagtaa gtgtaacagt tttctttttt ctttttttta ccataattac agcattcccc
    12001 cttttgtcga attgtatcaa aaatatatta taattatatc gaagaaagta gaaaaagtaa
    12061 accgacaaag ttgtaactaa gataactaaa gtgtaactta tatgacatta tttgcataat
    12121 attaaagtat catatatatt aaaaatatct ataaaaaaat ttagtatagg taaaaaaata
    12181 taaaaaatat aaaaatttat taaaaaggtg tttacaaaaa atgattaata tattactgtt
    12241 aaatagtgga tataaatttg agctcaaaat gtatgtaaat ttagatttaa aatactgaaa
    12301 aaatttacta tattaattta tagtaatgct tatagaaaaa ctctcttgct gtttaggggg
    12361 tttataattt tgcaaaaaaa taaaaaggag ggggctttat tgaaaaaata ctttggagaa
    12421 ataggactaa tatttatagc aattatatgg ggaagtggct ttgtagcaac ccaatttgca
    12481 ttagacggtg ggcttacacc tctacaaatc ataactttaa gatttttctt ggcagctata
    12541 attatgaatt tactgttttt taaacagatt cgtgccaata tgggtaaaaa attattaaaa
    12601 gcaggaggga ttttaggaat atttttattt ctagcattca cagtacaaac tattggactt
    12661 atgtacacta caccatctaa aaatgcattt ataacagcag ctaatgttgt aatagttccg
    12721 ttcataggat ttatacttta tagaagaaaa cttgataaaa taggaataat aagtagtttg
    12781 gtagctctta taggaattgg gatactttct ttagaagctg atttctctat taattttgga
    12841 gattttctaa cacttatatg ttcattcgga tttgcatttc atatattctt tacaagtgag
    12901 tttgcaaaag ataacaaccc aatggcattg acagctattc aatttacagt agcgttttta
    12961 atgtctgttg tagttcaaac atttgcaggt caattaaaga tggaagctga attatctggt
    13021 tatatgggaa ctatgtatct agctgtattt agtactacaa ttggattttt atttcagact
    13081 atatgtcaaa aaagagttga tggaactaga acagctataa tattatccac agaggctgta
    13141 tttggtacaa tattttctat aataattcta aaggaactta ttacagcaaa attgattatt
    13201 ggtagtatct tgatatttgt tgcaatcata actgcagaaa ctaaattatc ttttttaaag
    13261 agcaaaaaag tgaaacttaa agattcagag gagtcttcat tagaaagtat ttaatatgac
    13321 aactatttcc taattaatca aataagttat gtattatgta gaaatttgac atcaaaactt
    13381 ggttgatact ggtgtttcaa agtgataaaa ttaattagga aataaatttt atttttagtg
    13441 tttaagctat aaagtctggg gtatatatat tcttgtagca agttattaaa cttaaaaata
    13501 aaaatttaat tatataaaat tgggaggaaa ttaattatga aaaaatttgt ttgtacagta
    13561 tgtggatata tacatgaagg agatgctgca ccagcacaat gtccagtatg taaagttgga
    13621 gctgataaat ttgaagaaat gaaaggcgaa atggtttggg ctgatgaaca tagaatagga
    13681 gtagctcaag gtgtagatgc agaaataatc gaaggattaa gagctaactt tactggtgag
    13741 tgtacagaag taggaatgta tttagcaatg agtagacaag ctgatagaga aggttatcca
    13801 gaagtagctg aagcatataa gagaatagct tttgaagagg ctgaacatgc tgctaaattt
    13861 gcagaacttc ttggagaagt tgtagttgca gatacaaaag aaaacttaag agttagagtt
    13921 gatgctgagt atggtgcaac tgatggaaaa ttaaaattag ctaagagagc taaagaatta
    13981 ggattagatg ctatacatga tacagtacat gaaatgtgta aagacgaagc tagacatggt
    14041 aaagcattct taggattatt aaacagacat tttggaaaat aataattaaa acatagatta
    14101 aaaggagaaa ctttggtttc tcctttttta aattatacaa ataaactgtt gtgatagaag
    14161 gatggagata atatgaagtc ggtatctgtt aggagttgta aaacttacaa ttatgaagaa
    14221 gtaaagttat ctattgaaaa aaatttagag gatattggtg gtatagataa gtttgttaaa
    14281 aaaggaagca aagtacttat aaaaccaaat ttattaatga aaaaaaagcc tgaagaagct
    14341 acaactacac atcctatggt tgtaaaagtt gtatgtgaaa aacttttaga gttaaattgt
    14401 gatattacaa tagcagatag ccctggtggt ccatataatc aaaattctct taagagcatt
    14461 tataaggcat ctggaattga agaagtagca aatgaactgg gcattaagtt aaattatgat
    14521 gtttcagatg ttaagataca tagtaaaaaa gcacatgttc taaaatatat ggatattatt
    14581 aaaccaatag tagattcaga ttatataata aatctttgta aattaaagac tcatataatg
    14641 gcaaccttta ctggtggtac aaaaaactta tatggatgca tagcaggatt gaaaaaagca
    14701 gagatacatt atagatttcc tacagaagag ttgttttgcg aaaatgtact tttagatatt
    14761 tgtgattata taaaaccaac attgacaatt atggatggta tagtaggtat ggaaggtgat
    14821 ggaccttcag ctggtactac tcgagaaata ggagttttat tagcatcaca aaatccatat
    14881 gctatagatg ttgttgcatg taagattata agtttacttc caaatagagt tgctacaatt
    14941 agaggtgcta ttaagagagg ctatatacaa gaagattttt cagatattga aattttaggt
    15001 gaagatataa aagatttaat tataaaaaat tacaagattc caaatgtaag taaggattta
    15061 agattgtttt ccgtaaagct accaaaattt ttaaatgaac ctatttcaaa gcttataaca
    15121 ccaaagcctg tggtaagatt tcaagattgc ataaaatgtg gaaaatgcaa ggaagcttgt
    15181 cctgcatcca ctatagagat agggactaaa ggtgctatta tagacttaag taaatgtata
    15241 agatgttact gttgccatga actttgtcct aaaaaggcta ttgatgtcaa acgtaatttt
    15301 gtatttaagt atgtaaaata atgtataata ttatatgagt ctatataaat taaaaatata
    15361 aaggagaaat aatatgattt taattaaaaa tggtagagta atagacccta aaagtcaaag
    15421 agatgagaat attgacttaa ttataaaaga aaataaaatt tataaaatag gcaagtttga
    15481 tgaaagtgat gaatatgaaa aaataataga tgctagtggt aatgtagttg ctccaggttt
    15541 agtggatgta catgtacact ttagagaccc tgggtttaca tataaagaag atatagaaag
    15601 tggagctaaa tctgctgcta gaggtggatt tacaactgtt atatgtatgg caaatactaa
    15661 tcctattgta gataatgaag atacttttaa ttatgttaaa gaaaagtcaa aaaatgcatg
    15721 tataaatgtt ttacaagcag cagctataac taaaggtttt gaaggtaaag agttagttga
    15781 tatggaagct cttaaaaaag ctggtgtacc agggtttact gatgatggat tacctttaat
    15841 ggatagcaat ctaattatgg aggcaatgat taaggcaaaa gagttggatg taccacttag
    15901 ttttcatgaa gaagaccctt ctttagttgg aaatccaggt gtaaatgcag gaaaggtagc
    15961 ttcagaacta ggattaaaag gtgcatcaag tgtagcagaa gatgttatgg tagccagaga
    16021 ttgtatgttg gcactaaaga ctggtgctaa agttgatata caacatataa gttcaggtgt
    16081 atctgttgat atggttaggt ttgcaaaata cttaggtgca aatgtagttg cagaagcttc
    16141 accacatcac tttacattga ctgaggaaga tgtattggag tttggaacaa atgcaaaaat
    16201 gaatccacct ctaagaagta aatgggacag agataagatt atagagggat taaatgatgg
    16261 tactattgaa ataatagcaa cggaccatgc acctcatagt aaagaagaaa aagatagaga
    16321 gtttataaaa gctccaagtg gaattattgg gcttgaaact tctttagctc ttggaataac
    16381 aaatttggtg cataaaaatc atcttagtat gatgcagtta atagaaaaaa tgtctataaa
    16441 tcctgcaaag ttgtacaatt taaatattgg atttatagaa gaaggggctg tagcagacat
    16501 tgttatattc aatccagaag aagaatggac agttgaaagc tttgtttcta aagctgataa
    16561 ttcaccgttt aaaggtaaat ctttatatgg aaaggttaat tatacaatat gtaatgggga
    16621 gattgtatat aacgcctaat ttaagattat ttttttaagg cggagtgtac aatgattgaa
    16681 agtaaaagat tgtatatcag cagaatgaga ttgattaatc aaaattggaa gtattagttt
    16741 gaacaattag aacataacat cttggtaatt tagatatagt aaggaagaga atccttttta
    16801 tgaaagaacg cactattata tttggaaaga ttaagttcct aatctatcat atataatagt
    16861 gcgttatata tttaagtatg ttttatataa actaatctaa aaatcgaaaa tctttataca
    16921 atttctactt ataaattgcc tttatagggt ctagtttaga agctttaaag gctggaatca
    16981 gaccaaatac aactccagtc aatatagtag ctacaattgc ataaaataag ctatttaagc
    17041 taggtatagc ttcaaaacct atatatttag aaacatagtt agtagcagca aatccaacta
    17101 tagttcctaa aattccacca catactgtta taaaaacagc ttcaactaaa aattgaaata
    17161 atatagacct aggttttgct ccaatagcac gtcttatacc aatttctctt tgtctttcca
    17221 taacagatac atacattata ttcattacac caataccacc aacaaacatt gcaactacag
    17281 ttattataga tacatatttg tttacattgg aattaatgct ttcaagataa gctgtctgtt
    17341 cagttgggtc tggtgtagta taagaaccat tgataccagg gtgcatctca tataatttag
    17401 ctataacatt gtttgcaact tcatttacat tatagccttt agaagaaact aagtctaact
    17461 ggtaaatttc atttgaataa gaattttgac tcattaaagt gtcaaaagcc tttttaggta
    17521 ctagggaagt agtaaattgc atatcttggt aacctccaaa gaatcctcca gcttggtttt
    17581 gctgagaatc atctaatacc cctattattt caaatatagt tccatttatg ttgataccat
    17641 gacctaaggc atcttctgga ttttcaaaaa tttcacttgt actttgtaat gtaagtaaaa
    17701 ttacttttct tttttcatca tctaaagaaa aatctctacc acaaattaaa tttattttag
    17761 aatctttttt tactggtcca acatcaacat acgtagtttt tttatcaaaa gatgcttgtg
    17821 aagagtaaac tgaatcaagg ttaaacccat ctcttgaagg agctattctt tctactcctt
    17881 caacaaatga taattcttct aaatcttttg cattaaaggg ttttaaaaaa atactcatat
    17941 catcagtaag acctgtgtta tcggctgatt caaacgaaat agtagtttta ttagggttta
    18001 cattgtttac agatttctta atttcttttt gaaatccatt acctatagaa ctaacaagta
    18061 ttactgaagt tattcctatt ataatccaga gtaaagctac aaatacacga agcttgtgac
    18121 cttttaaatt ggccaaagaa ttttttaata aactcatgat tacacctccg aagtaaacac
    18181 accatcttta agtcgtataa ccttagttgc atacttagtt aaatcttgat catgtgtaac
    18241 cattattata gtcttacctt gtttgttgag ccttgttaaa agctccataa tttctctgct
    18301 agtttcacta tctaatgccc cagtcggttc atcagcaaag attatctgag ggtcatttac
    18361 taggcaacgt gcaatggcta cacgctgttg ttgaccacca gataattgaa gtggtttctg
    18421 cttaagcttg tccaaaagcc caacagaact taatttatct ttaacaattt cttctctatt
    18481 agattttttt agtgctttat tgtaaattag aggcaactct acattttgat atacatttag
    18541 agtttctatt aaattaaatt gttggaaaac aaatccaata ttaatatttc taaaaacaga
    18601 acgttcattt tcactcaaat tagttacatc tgttccatca aatatatatc ttccttcatc
    18661 aaatacatct agaaaaccta agatattaag taaagtagtt ttaccactac cagacttacc
    18721 cattatcatg acaaattctc cagattcaat ttctaaattt aaggatttta aaacatgtaa
    18781 ttcatctttc cctactttgt aatatttttg aatattttct aatttaatta acatatgtaa
    18841 attaattcac atccatagta acttcttcta cttttttgtt tggagtagta tctccattat
    18901 tagaaccagc agttacgcca tcgccttgaa ctgggtctcc ttctttcatt tctttagtag
    18961 ggtgttttat tattatatcg ccttgttcaa gaccgcctct aactacagca aaatcatcat
    19021 tttgacttgc tacatctaca atttgctttt tcaatatacc atctaaatcc ttaaatacat
    19081 atgagtgttt gttgtcttta agtacacaag atgctggtat tttaaagctt gaattagcta
    19141 cttcaagtga tgcttgaaga tggaatccat ttactaatcc ttcttgatta tcaaatgcta
    19201 tgtttatatc ataataagat agtgtattct gctgagctcc catttcagta tttggagtag
    19261 atggtttatc agatataaaa cttattctac cagtaagctt ttggtcagtt gaaaaaacta
    19321 gtatattgac catttggtca atcttcattt taggtaaatc ttgttcactt gcttgtcctt
    19381 tcatataaaa ctctagacct tgaatagtca taaatgaaac tggttggtct ggatttccag
    19441 tttggtcatt caagtatact gtgccatcaa aaggagcagt aactctgtca taagcttttt
    19501 tatccagaga agctatctga gagtttaaag cagttatctg tgcatttata gaagcaatag
    19561 catctttatt ttcagcaata tcagaaagtg atattttttg ttttttgtat tgactaagtt
    19621 gtgattttaa actatctatt tctgctatta tagttggatt tttagcagta aaaagaaggt
    19681 ctccttgatt tactttttga ccatttgtaa cattaacatc acttaactca taatctccag
    19741 atatattgaa gtccttagtt tgcttaggaa caaccatccc atttataaat attttttcat
    19801 tttcaggaat agtatatgtt tcgataaaat tttcattagc ttgtaattca gtgtttttcg
    19861 aatcaacata agcaagaact cctagaatta aaagtatgat tactgctatt cctgcatata
    19921 ttttgaatct cttattttta aagggattgt ttttaaaacc tttattttta gacttgaagc
    19981 cactacttcc aaaactgcta tttctaaagt tgttactttt aaaactgtta tttctaaatc
    20041 ttttattttt aaattttttt agaaaagaca tgtcttcctc ctttttataa aaactactta
    20101 attatatact gggatgtaca tatagaatta ctataagaag ttgaatataa gttaatgaaa
    20161 ataaactatc aataataaga tactataaat cacatttgaa tgtaatagta aattttgtat
    20221 attggttgta ctctgaatct acatcaattt tatgaccaag cttatcacaa agttctttta
    20281 ctaaataaag cccaagacct gtagatttag aattgtttct tccattatta cctgtaaatc
    20341 ccttattgaa tattcgattt aaatcttcct tttttatacc acaaccagta tcttccactc
    20401 ttaattgtaa ataatttttg gttcttttgc aatagataga tatattatct cctaatgagg
    20461 agtattttag ggaattactc aataattgct cgaatatgaa gtatagccat ttttcatcag
    20521 ataaaacctt aaaattcaaa ttatttaagt ctaaaataat tttttttgaa ataaaatatt
    20581 ttgagtattt ttttataata tttctaatga ttttttcaat atttacctct tgaattaaaa
    20641 aatccttatg aaaatcttcg agtcttgata aatataaaat tttttcaacg gagttttcaa
    20701 ttctaaatat ttcactatct atgtcatctg aaattttttc attaattaaa ttttcatttt
    20761 gttcaagaat gagttttatt atagctatag gtatctttac atcgtgtacc cacatattta
    20821 aaatctcttt gttttcttca aatttctcga tattattatt tattatgtta ttacattgat
    20881 attgatgatt tttaataatg ttcaagtata ttttttcttc atttttacat ctagctaatt
    20941 ctatatcatt catattaatt ttgaatgtat cattttttat cttatctaaa aatttgttct
    21001 tcttgatgta gctaataaat agatataaaa ataaaaacac aacatttaca actaatatat
    21061 ataaaatagt atcagtcaga aaaacatcta caggagaaaa tataataact gatactgtaa
    21121 aaattaagaa agtaaaattg tatactaaaa aacctagttt atccttgaga aaatctttaa
    21181 agctcataaa ttcacctact tagaattgtt ttaactgatt atataaccca atcctctttt
    21241 tgtttctata aaatcttcta aaccaatttc atttaatttt gagcgtattc tatttacatt
    21301 gacagttaat gtattatcat ctacaaacca atcattatcc cataattttt tcatgagttt
    21361 attcctactt actatctgcc ccttatattt cataagctca tgaagaattt tgatttcatt
    21421 ttttgttagc tcaatagtat tatcttcata gtttattgtt gcagttgata aatctaaagt
    21481 aacaccattg tgaataatct gatttgagtt attatcaaca aaattataag ttcttcttaa
    21541 aagggcatta actttagcta ctaagacatc tacagaaaaa ggtttttgta tataatcatc
    21601 tgcacctaaa tttacaccca ttattacatc catattagaa tttcttgaag aaataaaaat
    21661 tataggaact tttgaaattt ttcttatttc attacaccaa tgaaaaccat cataaaatgg
    21721 aaggtttatg tccaataaaa ctaattctgg gttatagttt ttgaactctt ctattatatt
    21781 ttcaagattt tctatttgat gaacatcatg tccccattta gtaagacact ttgctatgat
    21841 atttttcaat gagacgtcat cttcaactac cattatttta aacattctta cctcctaatt
    21901 caaaatagtt atattaacat aactatattc tacttgataa attaaaatat ttctacctta
    21961 ttacctaaaa taattatatt aatataacta tattttactt gataaattat attttaaaca
    22021 taattgcaat aaatttgaag aaatataatt aatacttacg aaaatgtaac cttaaattaa
    22081 tataattaaa tgtttaaaag ccattttaat atatataata tatttgtgag aattgataaa
    22141 taactaattg tgatgaaagg tgattattaa ctatggaaga aatattatct gttgaaaata
    22201 taaaaaagga gtatggaaga aaaggttcaa aacatgaagc tctaaaagga ataactttca
    22261 aggtttataa aggagaattt gttggtatta tggggtcttc cggtgctggt aaatcaacac
    22321 ttttaaatat tatttctaca atggatttac cttcatcagg agatatttat ataaatggaa
    22381 agaatactat taaaatgaag caaaatgaat tggcagattt tagacgtgat aatctaggct
    22441 ttgtatttca agattctaat cttttggata cacttacaat aaaagaaaat ataatgttgc
    22501 ctctttcttt aaaaaatgaa agagtatcag taatagaaaa tagaataaaa gagatttcaa
    22561 aagaattaaa tatagaatct atattagata aatatcctgg cgaagtttct ggtggacaaa
    22621 agcaaagagg agctgtatgt agagctatag caacaaaacc atcattagta cttgcagatg
    22681 agcctacagg agcattagat tctaaatctg ctagggattt gttgaattgt ctattaaaat
    22741 taaacaaaga cagcaataaa acaatattga tggtaactca tgatgctata agtgcgagtt
    22801 tttgcaatag aatactcttt ataaaggatg gaataatatt cacagaaatt gtaaagggag
    22861 aaagtaacag agagttctat aataaaatag taaatacagt ttccttaatt ggaggagtta
    22921 ataaaaatga ctttatttag aattgcaatg caaaatgtaa agaatagttt ttttaattat
    22981 ttaatgtact ttatttcaat tgtatttagt gtatttgtat tttttagttt taaatctata
    23041 gagtataatg aagcgttgag ttcacttgga gaaaaaacta ggatgagtat taatacaagt
    23101 tcaattgtaa tagtggtatt tgtattttta tttatatatt attcaaactc attttttttc
    23161 aatagacgta ggcgagaagt aggaacttat agtttacttg gaatgaggaa aaatcagata
    23221 ggaaaaatat ttttatatga aacttttttg atgggtatag ttgcaatact aataggtata
    23281 tttttaggac tgttattttc aaagcttatg actatgatgc ttgtaaaatt aatgaatgaa
    23341 atgatagtag taaatatgaa cttaagtata aaggccttag ttaagacact agcggtattt
    23401 ttaataatat ttatagttat tggaataaga aatactatag ttatcagaaa taagagaatt
    23461 atagagttat ttaataaata tccagaaaag tataagttta aaaaatttat aaagttaaaa
    23521 tgtgtacttg gtgttttatt aatagctatt tcttatttaa tgtcaattag ttattttatt
    23581 gctgaaaata ttatgctttc agtttttata ttagttacaa taatacctgg aactttttta
    23641 tttttttcat caactatgta tatgattatt gatgctgtta agaagagaaa aagtttttat
    23701 tataagggac agaatttaat tgcattttct gaacttggat ttaaattaaa gagcaatagt
    23761 agtgtattgg ctgtaatagc aatattaata gctacgagtg taactatgct agggtttacc
    23821 atatcacttt attacgatat agataggaac ataagtgaaa attataaata cagttatacc
    23881 ataaatttag gaaattctta tgtaaataac gaaattgaca aattattata taaatataaa
    23941 aaagataata agataatctt tgataaaaca atagaactta tagacaaaga tataaatatt
    24001 gaaatgtatt ataaaaatgg aaatagttat atagaagata gaacttctgt agatattatt
    24061 agagaaagtg attttaaaaa attaagacaa tatcagaata acaattatga agaattaact
    24121 tcaaaggaat ctgtgtacta tgtttctgat tcatacaaaa aaatgttttt taaagacttt
    24181 gagattgaaa aaataaactt gtatattaaa gatacaaatg agtatgatat aaatctgttt
    24241 aaggtacaaa aaagcatctt ggagccagaa ataaattcac aagctacgtt tgatttaatt
    24301 gtagtaaaag atgacgtatt taatcagtta aaattacatg gaaattctag attaataaga
    24361 ttaattgata taaaaaatga aaaaaaagaa ctggatttga ctttaaaatt taaaaatata
    24421 gtaagtgaaa atatgaaatt tacttatcct tttaatttta catcaagtat tgaaagttat
    24481 aataacttga
//
LOCUS       DACTGW010000040        24343 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.040, whole genome shotgun sequence.
ACCESSION   DACTGW010000040 DACTGW010000000
VERSION     DACTGW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.040"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            116..307
                     /locus_tag="I9573_001251"
     CDS             116..307
                     /locus_tag="I9573_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAT4795878.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            462..1871
                     /locus_tag="I9573_001252"
     CDS             462..1871
                     /locus_tag="I9573_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAT4795879.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIRIFKDKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            2002..2691
                     /locus_tag="I9573_001253"
     CDS             2002..2691
                     /locus_tag="I9573_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAT4795880.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2694..3629
                     /locus_tag="I9573_001254"
     CDS             2694..3629
                     /locus_tag="I9573_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAT4795881.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3626..4519
                     /locus_tag="I9573_001255"
     CDS             3626..4519
                     /locus_tag="I9573_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAT4795882.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4597..5343
                     /locus_tag="I9573_001256"
     CDS             4597..5343
                     /locus_tag="I9573_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAT4795883.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRASSRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5344..6177
                     /gene="rsmI"
                     /locus_tag="I9573_001257"
     CDS             5344..6177
                     /gene="rsmI"
                     /locus_tag="I9573_001257"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAT4795884.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6200..6904
                     /locus_tag="I9573_001258"
     CDS             6200..6904
                     /locus_tag="I9573_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAT4795885.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7092..7520)
                     /locus_tag="I9573_001259"
     CDS             complement(7092..7520)
                     /locus_tag="I9573_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAT4795886.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7557..8987)
                     /locus_tag="I9573_001260"
     CDS             complement(7557..8987)
                     /locus_tag="I9573_001260"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAT4795887.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9119..9703
                     /locus_tag="I9573_001261"
     CDS             9119..9703
                     /locus_tag="I9573_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HAT4795888.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9719..10240
                     /locus_tag="I9573_001262"
     CDS             9719..10240
                     /locus_tag="I9573_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HAT4795889.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     gene            10636..12573
                     /gene="metG"
                     /locus_tag="I9573_001263"
     CDS             10636..12573
                     /gene="metG"
                     /locus_tag="I9573_001263"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAT4795890.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12603..13373
                     /locus_tag="I9573_001264"
     CDS             12603..13373
                     /locus_tag="I9573_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAT4795891.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGICYEEVCRVTKENAKKFFNIK"
     gene            13710..14141
                     /gene="phnG"
                     /locus_tag="I9573_001265"
     CDS             13710..14141
                     /gene="phnG"
                     /locus_tag="I9573_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAT4795892.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14156..14731
                     /gene="phnH"
                     /locus_tag="I9573_001266"
     CDS             14156..14731
                     /gene="phnH"
                     /locus_tag="I9573_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAT4795893.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTVSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14734..15819
                     /locus_tag="I9573_001267"
     CDS             14734..15819
                     /locus_tag="I9573_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HAT4795894.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15839..16708
                     /locus_tag="I9573_001268"
     CDS             15839..16708
                     /locus_tag="I9573_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAT4795895.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16701..17567
                     /locus_tag="I9573_001269"
     CDS             16701..17567
                     /locus_tag="I9573_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4795896.1"
                     /translation="MSKINVSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17608..18321
                     /locus_tag="I9573_001270"
     CDS             17608..18321
                     /locus_tag="I9573_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT4795897.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18322..19491
                     /locus_tag="I9573_001271"
     CDS             18322..19491
                     /locus_tag="I9573_001271"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAT4795898.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDRIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVLNTIVNGKTVYKREV"
     gene            19495..20331
                     /locus_tag="I9573_001272"
     CDS             19495..20331
                     /locus_tag="I9573_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAT4795899.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20332..21327
                     /locus_tag="I9573_001273"
     CDS             20332..21327
                     /locus_tag="I9573_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT4795900.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLDVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21345..23033
                     /locus_tag="I9573_001274"
     CDS             21345..23033
                     /locus_tag="I9573_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HAT4795901.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYIIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23045..24112
                     /locus_tag="I9573_001275"
     CDS             23045..24112
                     /locus_tag="I9573_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAT4795902.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9714 a         2710 c         4346 g         7573 t
ORIGIN      
        1 ttttttattt tttgtcaaaa aaaacaaaaa atacgtattt attttttcat ttcgggaaaa
       61 ctaaacacag gaattatgga ctttataaaa atttaattct tgaggagtga tataagtgaa
      121 aaaagttgct ttaattttag ctataatagg tgcgcttaac tggggtgcaa ttggtattct
      181 aggtacagac ttaataggta gtatctttgg tggaacatat gaaatggtaa gtagaataat
      241 atacttcatt gtaggtttag ctggattata cttaatacca agtttaatgt ctgatgatag
      301 agaataattt atgcttttat gttaaatacc tcattctaaa tgtttagaat gaggtattta
      361 attttataaa aatattaatt tgtttctttt ttaatggtaa aatagataag aattgaagtt
      421 ttaaaaagaa gtattatgta aaaaaggagt actattaata tatgtttatt attaatgaat
      481 taaataagat tgtaaatgaa gaagtaatat cttttcatat gcctggtcat aaaaagggac
      541 aggtttataa aaagttagga tataaagata tactaaaaaa tttgtataaa atggatacaa
      601 ctgagatatt aggaacagat aatttacact ttccggaagg aattataaaa aattcacaaa
      661 aaaaagcatc aagagtattt aaaagtgatt atacttgcta tttagtaaat ggtagtagct
      721 gtggaataca atctgcaata atgtcagtat gtagtcctaa aagtaaaata ataacaaata
      781 gagactgtca tcagtctgtt ataaatggat gtattttagg agatatagat atagagtatg
      841 tgccttgtga gataagtgaa gagacaaatg tcttaaaagg gatatctata agtaatgtga
      901 ttgatataat agataaaaac ttggatgcaa aagcagtact gcttacttat cctacatatt
      961 atggaatgac atatgattta gaatacatat gtaattatgc acatagcaaa aagatggtag
     1021 tgatagtaga tgaagcacat ggtgcgcatt tagggcttag tgagaggctt cctaaaacag
     1081 ctcttgagca aggtgcagat atagttgtac agagtacaca taaaactctt ccagcattta
     1141 cacaatcgtc aatgatacat atacagggag accgagttga tatagataaa atattgaaca
     1201 tgttaagaat tacagaatct tcaagccctt catatttatt attatcttct ctggaattag
     1261 cagtagatat atatgaaaat aaaggaaaag aattaatgga agagctttta aataatatta
     1321 gaattttcaa agataaagtt gattcaaata ttaatatata tgacacaaat gataaaacaa
     1381 aaatatttat atcatctaaa aacatgggtt taacaggtta tgaactagaa aatattttga
     1441 gaaacaaata taacatacaa gtggagttat ctaattatta tggagttctt ttaatatgta
     1501 caataggaaa tactgctgag gatttctcta gtttagaaac agcattaaac aatgttgtaa
     1561 aggaagactt taaatttgaa aagttagaaa ataaaaaata ccctgttgtt attccaaaga
     1621 aaatattaac acctagagaa gcattttata aaactaaaaa aagtgttaaa atatatgaca
     1681 gcataggtaa aatttgtggt gaatgtatag ttccataccc acctggaata agtgtaatat
     1741 ctcctggtga agtaataagt aaagaaataa tagactatct aatgttttgt cattctaaag
     1801 gcatgattgt gagtggattg aaagatgtag atttaaattt tatacaagta atagatttag
     1861 aagacattta aaaattatga tataaaagta gatagaagta gatagtttaa aattgtgaag
     1921 agaaataaat agactgctta tatagagcta tctaataaaa gtactaaaaa agtaaaaagt
     1981 gtttgtatgg aggatatgaa aatgaaaggt aagttaataa ttatagagag tggttcagat
     2041 gcaagtggaa aggctactca gacagaaata ttatatgaaa gattaaaaaa agaaggctat
     2101 atgattaaaa aggtagaata tccaaattat gaatctccat catctgcctt agtgaaaatg
     2161 tacctaaaag gagactttgg taaaaatgca tcagatgtag acccatatat agcatcaact
     2221 ttttttacag ctgataggta tgcatcattt aaaaccgatt gggaggagtt ttacaatgaa
     2281 ggtggaataa ttatcgcaga tagatataca acctctaata tggtacatca ggcctctaaa
     2341 atggatattt gtgatagaga agagtattta aattggttag ctgattatga atttaattta
     2401 tttaagatac cacaacctga ttgtgttata tttttagatg tgcctattga atttagtaaa
     2461 aagcttatgg agaatagaaa aaataaaatt acaggggaag aaaaaaagga tattcatgaa
     2521 agtgacatag agtatttaga gaaatcttac aacaatgctt tatatatagc agataaatat
     2581 gattggaaaa aaataaattg tgttttagat aacaaattaa gaagtattga aagtatacat
     2641 aatgaaattt ataaaatagt tcttgattca ataaaaccta tggagagata aatatgtatt
     2701 ttgaaaatat tatagggcaa gattttgcaa agaaatattt aacaaattct ataaagaaaa
     2761 ataaacttaa taatgcgtat atgtttgaag gaatggatgg aataggaaaa aagaaatttg
     2821 cagatgaatt atcaaaacta ttacttgatt atgaaaatct cgaaaatagt ccagactatg
     2881 tactcataaa accagatgga aatagtataa aaatagctca aatcagaaat ttacaatcag
     2941 atatagtaat aagacctcat aaagactata aaatatatat aatcaataat gctgaaaaaa
     3001 tgactgtgga agctcaaaat gcacttttaa agacgctaga ggaacctcca aactatgcta
     3061 taataatctt agtcacaaac aacaaggaat ctttattaga aactataaaa tctagatgtg
     3121 atataataaa gttctcacct ataccaattg aagacttaaa acgttatttg ataaatacag
     3181 gtatagaaga agaaagagct caattattgg ctattttttc gagaggaagt attgagaatg
     3241 ctctaaatct atctcagtct tctgagtttt caatgatgag agaagatata caacagtata
     3301 ttcagataat gttagataaa aacatagtgg agattttaaa tataccaaac aatatggaaa
     3361 aatatagagg taagattata gctttattag atatgatgat aaactatttt cgagatatca
     3421 tcctattaaa agaaaatgta aacaagaaca tgttaataaa tgtagataag ctagttttca
     3481 ttcaaaatat gagtggaaaa attagttatt ctcaattgtc caagattatt gatataatag
     3541 aagatgcaaa gagtaaaata aaaagcaatt gcaatttcaa tataagcata caagttatgt
     3601 ctttaaatat atacgaggtg attaaatgat aaagatagta ggtataaggt ttaaaagtgc
     3661 tgggaaaata tattattttg accctgttga ttttaatata gagcaagata tggatgtagt
     3721 tgtagaaact gcaagaggct tggagtatgg gaaagtagtc gttggtccaa aagatatgga
     3781 tgaaagcgaa ttagtttctc ctttgaagcc aattataaga atagctacag aagaagataa
     3841 aaaagtatat ctagaaaata aggaaaaggc aaaagaaact tttgagttgt gccaacaaaa
     3901 aataaaagaa catgagttaa caatgttttt aatagattgt gagtatacgt ttgataggaa
     3961 taagttgatt ttttacttta cagcagaagg aagaatagat tttagagaat tggtaaaaga
     4021 cttagctgct atatttaaaa ctagaataga acttagacaa ataggtgtca gagatgaagc
     4081 taaatctata ggaggtctag ggccttgtgg tagaaaactt tgttgttcat cttggttagg
     4141 tgattttcaa ccagtttcaa taaaaatggc aaaagaccaa agtctatctc taaatccaac
     4201 aaaaatatct ggaatatgtg ggagattatt ctgttgctta aaatatgagc atgatgtata
     4261 tagtgaagca atagaaaaaa tgcctgttgt aggtgcattg gtgcaatcag atgatggaaa
     4321 aggaaaagtt gtagaagtga atcctttatt agaacaaatt aaagtggaat ttcaagacaa
     4381 gacagtaaag atatacacaa gagaagatat aaaaatactt agagagccta aaaaatgtga
     4441 tggctgtggt ggtaaatgta aggataatga tggattagat gaagccacac taagagagct
     4501 aaagaaatta gaagattaaa tttaaggtag ggcttttcct acctttttta aagcaattaa
     4561 tgcttaaagt tattaatatt tgaggagaat ttatatatgc aattaaaaga ttcagaaaga
     4621 atagatgatt tacaattaaa aggccttaaa attatacaag atactaatgg tttttgtttt
     4681 ggtatagatg cagttttatt agccaatttt actaaaataa aaaaagatgc caaagtagta
     4741 gatttgggta ctggaactgg aattatacct attttgatag ctggaaaaag tgaagctaaa
     4801 aaaattattg gcgtggaaat acaagaagat gtatatgaaa tggcaactcg ttctataaaa
     4861 ttaaatggat tagaagaaag agttgagatt ataaatgaag atataaaata catagacaaa
     4921 gtcttagaca taaatggtta ccatgtagtc acatcaaatc ctccatatat gcatattgat
     4981 ggaataaaaa atccaaatga taaaaaagca atatcaagac atgaagtaag gtgcaatttg
     5041 gaagatgtaa ttagagcttc atccagattg gttatgccta gaggaaaatt cttcatgata
     5101 catagaccaa caagattggt tgatataata actttaggga gaaaatataa attagaacca
     5161 aaggttattc aatttgtgca tccaagacct caaaaagcac cgaacttagt tttggttcaa
     5221 tttgtaaaag atggaagacc agagcttaaa atattggacc cattgtatgt ttatggagaa
     5281 gatgggaatt atacaaaaga attaaaagca atatataata ataaagatat aggagagttg
     5341 taaatgagtg ggaaattgta catatgtcca acaccaatag gaaacctaga agatataaca
     5401 tatagaactt taagaacatt aaaagaagta gacttgattg cagcagaaga tacaagacat
     5461 agtataaaac ttttaaatca ttttgaaatc tcaaaaccac taactagtta tcatgaacat
     5521 aataaggatt caaagggaga ttatttgata aataaactaa ttgatggtga aaatatagct
     5581 ctgataagtg atgcaggtat gcctggtata tctgacccag gagaagagat aataaaacaa
     5641 gctatacaaa ataacataga gatagaagta ttgccaggtg caacagcctt tgtaacggct
     5701 ttagttggct ctggtatgga cacacataga tttgtttttg agggcttttt agatagagat
     5761 aaaaaagtta ggaaagcaca attggaagaa ttaaaggaag aaagtagaac tataattttc
     5821 tatgaatcac cacatagatt gaaggataca ctaaaagata tgcttaaaat tcttggtaat
     5881 aggaatattt ctataaatag agaacttaca aaaaaatatc aagaaataat aagagaaaat
     5941 atagaaacta ccataaaaat atttgaggaa aaagaagtaa gaggagagtt tgtattaata
     6001 gtagagggtt ttcatggtga gaagtcaaag aaagaaaatt atgatgggct tacagatagg
     6061 gaatatgttt taaaactaat tgaaagtggt atgaacaaaa aagatgcaat aaaaatagtt
     6121 tgtaaagata gaaagttaaa gaaagatgtg gtttataaac aagttttaga tttataaaac
     6181 ctgaatctga ggtgatttta tggaaatagg gaaaattata atagagagtt taaagaggga
     6241 agatacacat aaaatattta tagaattaga taataagcat gaattagaaa aaataattcc
     6301 taaaataagc tgtatgaagt ctgtaggtga atgtaaatat catgtagtaa attgttttga
     6361 acattctata aatgcattaa aagaattaga aatagtatta aatgataagg atttttttcc
     6421 aatacatcta agaaaacatg tgtcaaacta tttaaacaca tatattgaag aaggaataaa
     6481 taagttacat gtattaaagt tgggaacatt tttacatgat ataggtaagc ctgatagtat
     6541 gaccttagat gaaaccggaa gagttcattt cacaaatcat gaaaaaatag gtgctcaaat
     6601 aattgacaat atgggaataa agttagagtt atcaacagag acatatagac ttataagtaa
     6661 atatgtaaga tatcatatga tacttttgtc tctatataag aaaaatgatt tgagtaggaa
     6721 agaattaatt aatgtattta acttagtaga tgaggacact ataggtgtaa taatgcttgg
     6781 gtatgcagat atagtgtcta cagtaaaatt attgggcaag agtggagaag tcagtgtgtt
     6841 aaagacatat atggaatata ttttaacaaa ttacttgtat aagagtaatt atacacatgt
     6901 ataaatttag ttaatataat tgagttttag atgattaatt gaagtttgaa atatacggat
     6961 ttcaaaaaca taaaagaagt tgtctttgtt gtaaaattat gaaagacaac tttttttata
     7021 tcaaaaattg aaattttatt aaggatactt aatggtctat taattaataa accattagta
     7081 tttaatttat attacaatat tgattttgct tgtattggcg ttgatagtat aacagaggtc
     7141 ttagtagaac caattttctt tatagtatca atcacatttt ctagctcgta catatctttt
     7201 acaattactt ttaaaagtaa gcaatcatca cctgttatat ggtgacattc tacaatccta
     7261 gggtcctttg cagctgactc aataaattct gtatatccat tgcttggaag agaaatatga
     7321 ataaatgcct ttataactct gcctaatgaa tctgggttga caatagcttt atatccttct
     7381 ataacaccag actcttctaa tcttttgact ctttctgaaa ctgcaggaga agttaaacca
     7441 actatttttc ctaagtcctt catagaaatt ctcccatcat cctgtaaaat ttctatgatt
     7501 ctgtaatctg taacatccat aaataaacag tcccccttaa ataaaaataa ttaatactat
     7561 aaaacttttt tagaatctat catttgtgtt tttaaatacc aaagatttct agataaatca
     7621 accttataaa tgtgtggttt tgaaagtctt ttgtattgtt cccagatagt acttaattca
     7681 tattgtacat gattttttat ttcaagaaca gcttttttat tatatttgca tctgcctttt
     7741 atatacaatg gtttaagaag ctctttaact ttgtaatttg taaatacctt tgttttccat
     7801 gtataagtag gatggaagat ttctaaaggt ttagactcat ctataacttc atcatgtaac
     7861 ataatcaaat ctgcctctgc cttattgtct tcgttatata ttcttataac tttcttgtaa
     7921 ccaggattat ttattttttc tggattctca gaaatcttta tcttaggttc aagaactcct
     7981 ttttcacaag atgcagctaa tttataaact cctccaagag aaggtgaatc gaaagatgta
     8041 atgagctttg tacctactcc ccatgaattt atagtagctc cttctgcttt aagtgatgtt
     8101 atggtatatt cgtctaggtc attagatgca gtgatgctga tatctgaaaa gccagcgtta
     8161 tctaattgtt ttttagcttc tttagataaa taagctaaat ctccagagtc aagtcttata
     8221 cccataggtt tgtatccttt ttcagaaata tttttgaata cttttattgc attaggaact
     8281 ccactgttta atacatcata agtatctaca agtagtaggc atttatctgg ataaacatca
     8341 gcatatgctt ggaatgcttc aagttcatta tcaaattttt gtacccaact gtgagcttgt
     8401 gtaccaatta taggtatatc aaacattttc cctgctagta cgtttgcagt tccagcacaa
     8461 ccaccaacta cagcagccct agctccatat atccctgcat ctggaccttg tgcacgacgt
     8521 aatccaaatt caaatacagg gtctccttga gctgcaaagc aaactctaga ggctttagta
     8581 gcaataagag actgaaaatt aactattgtt aacaatgctg tttcaataag ttgagcttga
     8641 tataaaggtg ccttaacagt aattaatggc tcattaggga acatgatagt tccttcctca
     8701 acagcatata tatctcctgt gaatttaaat tcttttaaaa attttaaaaa cttgtcagaa
     8761 aataaattta agttttttaa gtactctaaa tcttcatctg aaaaatgaag attatctatg
     8821 tattcaacaa cctgatcaat accacaaact atagtatacc ctccatcaca agcgtttttt
     8881 ctaaaaaaca tatcaaatac aacaatgtct tcatgaatat tcttttcaaa atagccattt
     8941 aacatagtaa gctgatataa atcagtaaga agtgttagat ttctcattat ttctctcctt
     9001 aattattatt aaatattaaa aattgtaaaa actcccacac ttatactaaa acataatata
     9061 gtacaacttt caagtataaa gtattaccta actatcataa aattatttaa agaggaggat
     9121 gattataata ggaaaaatat ggttttatat ggtatcgata ggaataatag gaagcttgat
     9181 ttctaaaaat atgggtgaat taaataaagt tatattaagt gaaacatcaa aaggtataga
     9241 gtttgcaata agtctagctg gtatcatggc actctggatg gggataatga atatcgcaaa
     9301 agaatcaggt ttaattgaaa agataggtca aaaactaaat cctataatga gaagattatt
     9361 tccttctatt ccaaagggtc ataaagcaat gtcttatata gttatgaata ttgctttaaa
     9421 tatgttgggt gctggaaatg gtgcaacagc atttggttta aaagcaatga gtgagcttca
     9481 aactttgaat aacaagaaag atacagccac taatgatatg attatgttta tggtaatcaa
     9541 tatatcatct atacaaataa ttccatttac tataatcaaa ttaaggatgg atatgggggc
     9601 tcaaaatcca agtgaaataa tttttacgac tttatttgca accataatat ctacagcagt
     9661 agcaataata acatgtaaat tttttcaaag aagatataga taaaggggat gtagatacat
     9721 ggagatattt tcaaacttac ttataccaat aataattctg tacatagtag tttatggtaa
     9781 atataaaaaa atagatgtgt atgatagttt tgtaaaagga gcaattgatg gattaaaagc
     9841 agcatgggat atcctaccat atataatagg tatatttttg gcaataggaa tattcaaaac
     9901 tggtaaaggt cttgatatgt tagaatggct atttacacca atagcaaata tgatgagtat
     9961 accaaaagaa ttaataggtc taataagtgt aaaaccttta tctggaagtg gtgctttagg
    10021 gatgtacagt gaacttgcaa atagagttgg aataggaagt ttagtagaaa aaatgggtgc
    10081 tacgattgta ggttcttctg agactatttt ttacacaatg gcaatatact atggaagtct
    10141 gaagataaaa aatactagac atacattatc atgtgctatg attagccatg ttgcaggtgt
    10201 catagcagct gtttttattt gctatgtaat atttgtataa tttgttgaca atatgggaaa
    10261 aattttataa tatagtgatt atatagaaac aaatagtaat acaaattaca agctatgaaa
    10321 agaagagtaa ataaaggttt tttctatcag agagctaggt taggtgaaag ctagtgtcaa
    10381 aagctttatt gaagatggtc ttggagcttt ttatctgagc aagtgtgcta gggtaaaacg
    10441 gttggacacg ttatagtcta ctaaggtatc tataaaatgt ttctacattt catgtgaaac
    10501 ataactatta tagatgaatt agggtggtac cgtgaataat ctcgccccta tgttaactca
    10561 taggaggcga ttttttatta tttaaatgaa agaatcatat gattacgaac gataaaaaat
    10621 taggaggtaa ataacatgag caaaccgagt ttttatgtaa caacaccaat atactatcca
    10681 agtggaggat tacacatagg acatacttat tcaacagtag ctgcagatac aatagctaga
    10741 ttcaagcgtt tctgtggata tgatgtaaag ttcttaacag gaacagatga gcatggtgaa
    10801 aaaattcaaa agaaagccat agaacaagga atgtcagaga tagagtatct tgatggaatg
    10861 ataaaagata taaaggcctt atggaatact atggatatat cttatgatga ttttataaga
    10921 actacagaaa aaagacatac agacataata caaaagatat ttactaaact atatgagcaa
    10981 ggtgatatat ataaaggtga atatgaaggt agatactgta ctccttgtga gagtttctgg
    11041 acagaatctc aattactaga aggaaataaa tgccctgact gtggaagaga aacatattta
    11101 gtaaaagaag aatcttattt ctttagatta tctaaatatg aagacagatt aaaagagtta
    11161 tttaaagatg atagtttctg ttttcctgct gctagaaaaa atgaaatggt ggcaaacttt
    11221 ttagacaaag gattagaaga tttaagcgta actagaacga cttttgactg gggtataaaa
    11281 gttccttttg atgaaaaaca tgtaatttat gtgtgggtag atgctttatg taactatata
    11341 acagcattag gttatatgac tgataatgat gaagaattta aaaaatattg gcctgccaat
    11401 gttcaaatag taggtaaaga aatagtgaga ttccatacaa taatatggcc agcgctttta
    11461 atggctttag gtctagaagt tccaaaacaa gtatttggac atggatggat actatttgct
    11521 gatgataaaa tgagtaaatc aaaaggtaat gtggtttatc cagaacctat aatagaaaga
    11581 tatggaatag acacacttaa gtacttctta ttaagagagt ttgcatttgg gcaagatgga
    11641 agttatactc atagaaattt tgtaacgaga ataaattatg atttagcaaa tgatttagga
    11701 aacttaataa gtagaactgt agctatggtt gaaaaataca ataatggtat aataccaaca
    11761 gcaaaagttt caactgattt tgatgctgac ttaaaagaac aagctgtttc aactagagaa
    11821 aactttgaag ctgaaatgga taaaatgcaa ttccatgaag cattagagag tgtatggaaa
    11881 ttagttagaa gaactaacaa atatatagat gaaactatgc cttgggcact agctaaagat
    11941 gaaacgaaga aagatgaatt agacacagtt ttatataatt tatgtgaatc tataagaata
    12001 atcgcaacac taataaatcc tataatgaat gagactgcta ataaaatata tgaacatata
    12061 ggaataaaag gtcaagatga tataactaca tgggaaagta caaaaacatt tggtcttatt
    12121 ggagaaaatg taaaagtatt taaaggtgaa cctttattcc caagattaga tgtagaaaaa
    12181 gagatagaag aattaactaa aatgttctct gggaaacctc cagtagaaga aaaaccttta
    12241 gagcataaag aagaaattac tattgatgat ttagacaaaa tagagcttag agtagggaaa
    12301 ataataagct gtgaaaaaca tccaaaagca aataaattat tagtatcaca agttaagatt
    12361 ggaccagaaa caagacaaat agtatcagga atagctgaat actataagcc agaggattta
    12421 gtaggaaaag aggttacagt ggtatgtaac ttaaaaccag ttaaattaag aggtgtagaa
    12481 tctcaaggta tgatattagc agcaggtgat gatggagacc catatgtttt accatttaca
    12541 caaggggcta aagatggatg cgaggttcgt tagtaggtaa ttaaatagta aggagattaa
    12601 agatgttatt tgactcacat gcacatttaa atgatgaaag ttttgatgaa gatagagatg
    12661 aacttattgg ttcattaaaa gataaaggtg tagatttggt tgtaaatcca ggagcagata
    12721 tagagacgtc gataacagca attgaacttg cgaaaaaata cgactttata tattcagcag
    12781 ttggggtaca tccacatgat gtatcaaaac tagatgatac agcaatagaa actctaagaa
    12841 aacttgcaac tgagaatgaa aaagttgtag ctattggaga gattggatta gattattatt
    12901 atgattattc tccaagagaa gaacaaaaag agtggtttaa gaagcaaatt gaattagcta
    12961 atgagctaaa acttccaata ataatacatg atagagatgc acatggagat acttttgaga
    13021 taataaagaa tactaaaaat cctgaaatag gatgtgtact tcattgttat agtggaaatg
    13081 tagagttagc tagagaatat gttaagatgg gttgttatat atcaattcca ggcacggtta
    13141 cttttaagaa taataaaaaa actagagaag tagtaagaga aatacctcta gaaagattat
    13201 tcatagagac tgattcacca tatatgtcac cagagccaca tagaggaaag agaaacaatc
    13261 cttctcaagt atcttttgtt gctgataaga tagcccagga aaaagggata tgttatgaag
    13321 aagtatgtag agttacaaaa gaaaatgcaa agaaattttt taatataaaa tagacaaatt
    13381 aaatcttata atgatatata taataagtca ttataagtat aggatgagag tgcctcaaaa
    13441 tgagtttttt agttcatgag gcactctttt tttatgaaat caaatatatt ttcatcattt
    13501 caacaatgaa aaagaagttt atcatatttt gagacagttt gtttttattt gtttaattca
    13561 tattttaaaa tatattttct taacattgtt aaaaaagaat ttacaagata ttcattagaa
    13621 ttaactcaac tctaatattc acttaactta agtggtttat tatattatta aagagaagta
    13681 aacgaaaggt agattaagga ggctaaattt tgtatacagt tgatagtact aagatattga
    13741 taaatatgaa cttagagaaa ctagaaaaga tatactcaat gataaaagat acatcaagta
    13801 tttctataat taaagaacct aacttggcta cagtaatggt aagagctaat gaaagtgtaa
    13861 aaaatacaac atttaattta ggagagatat tagttactga atgtagtgta aaggtagatg
    13921 aaagcttggg ttacggaata gtttcagaaa acaataataa gaaagccatt tacttagcag
    13981 ttatagatgc agttttacat agcaataaca gtaaatttga tgagttaaag aattacataa
    14041 ataaaagtgt atatgaagag aacttaaggt atgaacaaga gattattgat gaatttagct
    14101 tgattaataa aacgaaagtt caattcaatg ctatggatta aataggagga gaatcatggt
    14161 aaattataca caagaaatat atagaaagat gctagatagt gcttcgtttc ctgggaaatt
    14221 aaataatata gataatataa atatagaaaa taatacaaaa ctattgaatg gagcaattgg
    14281 tatagcaatc actttattag accaagaagt taccttctat atagaaaatt atactcaaaa
    14341 agatataaaa aatataagag cgcttacagt gtcaaatcaa atagattata gaaaatcaga
    14401 ttatatattt ttagatatta attcagaagt ggatatatta cagattaaaa taggttcttt
    14461 tgaatatcct gatgaaagtg caactatagt acatcaagta aatgatttat caacgtactc
    14521 tcaaactaag tatataaagc ttgatttgag tggtcctggt ataaaatcta aaaatagtat
    14581 atatataaat ggaattagag aagaatttat agaaagatta tctaccataa ataaagatta
    14641 tccaatagga atagatttga ttttagtaga taaaaaagga gaaattgcat ttataccaag
    14701 gtcatcaaaa ttaagttggg aggtattgta gaaatggctt atgtagcagt taaaggtgga
    14761 gaagaagcca tattacaggc taaaaatata cttgagtttt atagggtaaa aggagatagc
    14821 ttgcctatag atgtaaagca gattgaggaa caaatgtctt atgctgtaga caaagtaatg
    14881 tctgaaggaa gtttatacaa caaggaacta tcagcattgg ctataaaaca agctcaaggt
    14941 gatatattag aagcctcttt ctatgtaaga gctttaaggt caacaatgcc tagaattaca
    15001 tattcagaac ccataaatac agaagaaatg agaataaaaa gaagaatatc agctactttt
    15061 aaagatatac caggaggtca gtatcttggg gcaactagag actatgaaaa tagaatatta
    15121 aatgtagaat tattagaaga agatgaaaaa aagaatagag attttagaga tagttttata
    15181 aatgaattag aaacagtaga tgaaatccct gaatttacaa aaatatctaa tatatttaga
    15241 aaagaaaatt tattacaaga agaagtttgt gaaaacaaat tggaagaagt tattgatgat
    15301 attacaagaa atagtttaag atttccagtt tctaggtcag caaagttaca ggcattgact
    15361 agaggtgaaa gtggagtagt gtcaggtttt gcttatgcta ttgtaagagg ttttggtgat
    15421 gaacatccat atataagtga acttagaact ggtaatgtat ttataaaagt aaaacatccc
    15481 atctatgaag atgaagaaat aataataggt gatatgctgg ttactgaatg tgaaatagtt
    15541 tcaaaagcat cacaaaataa gactgaaaat tctaaaacta attcggaaga taaaaataag
    15601 atgtctttag gatatggtct ttgtatgggg aataatgaga ataaggctat atctatggcc
    15661 atactagaca aatctttgga gccaaatagt gaaaaactag cacaagatga agaatttgta
    15721 ttgcttcata tagatggaat agattcatta ggatttatat ctcactttaa gcttcctcac
    15781 tatgtagatt ttaaagcgga tgtagaaaga ataaaatagt ttgtggaggt aagtttaggt
    15841 gatatataac tatggttttt tagacgaaaa cacaaaaaaa gaaataagaa gaaagatatt
    15901 aaaagcaata tcaatacctg gttatcaagt tccctttggt tcaagagagt tgcctatagc
    15961 taagggatgg ggtactggag gtttacaatt aacactatca ttaattggta aaaatgatgt
    16021 agtaaaagta attgaccaag gaagtgatga ttctacaaat gcttgtaata taaggagctt
    16081 tattgcaagt gtaagtaatg tagaaaccac aaaagataca ttagaagcta cattgattca
    16141 aacgagacat agaataccag aagaaaaact taaatctaat caaatactaa tatttcaagt
    16201 accaatacca gaaaccctta gaatagtaga acctagtgaa gtagaaacaa gaagaatgca
    16261 ttcagaagaa gattatagta ggatgtgggt atatctttat gaagatatag ttagatttaa
    16321 tgatatatct atagcagttg aatatccatg taaagtaaat gatagatatt taatgaatcc
    16381 atcacctata cctagatttg atataaaaaa attaaatatg tcagacaatt tattcttgtt
    16441 tggtgcaggt agagaaaaaa gaatttatgc tattcctcca tatacaaagg tagaaccttt
    16501 agagtttgag gattataagt ttgaagagga aaagtttgaa ggaaaatact gtagtttatg
    16561 taacagtaca aatacatttt tagatgaagt atatgacagt gacactaatg aaaagtacta
    16621 tagttgctca gatacaagtt attgtgaaaa agttagatta aaaaataaca gtatagatgt
    16681 gacaatagga ggtacttgga atgagtaaaa taaatgtctc taaaagatta gaaaatatag
    16741 acaagcaagt attaatagga agaaatatca atcttattta tggagatggg tgtgaaaagt
    16801 gttttgaaag cacaggatat gaaagcaata atatatgtaa gtattgtggt tctatagttg
    16861 catgtaataa tgtaaattta gaactttatg aaggagaagt ccttggaatt gtaggtgaat
    16921 ctggttcagg aaaaagcact ttattaaaaa tactgttttt ccaagaacat gcaaatagtg
    16981 gagaagtata tatatcccat tacaataaag acacaaatat actagagctt tcagaccaaa
    17041 aaaagagata cataaagaat cattttatgg gtattgtgta tcaaaatccc caccttggtt
    17101 taaatttaga ctttagtagt ggtggtaata tagcagaaaa attattgatg gcaaatttat
    17161 ataatgttga aaaaataaga gatagaggca aagaactttt aataaaaact aatatacctg
    17221 tagaaagaat agatcataaa ccaaaatact ttagtggggg aatgcaacaa cgagttcaaa
    17281 tagcaaaagc tctttcaaat aaccctccaa tattgctatt agatgaggtt acaacaggtt
    17341 tagatgtttc tgtgcaagcg gaagtattgg atttaataag agaaatccaa agagaactta
    17401 agatttctat gatagttgta tcccatgact ttgatgttat aaagatgctt gctgatagga
    17461 caattgtaat gaaaaatgga agtgtgattg agtctggtct tacagaccaa ataatggagg
    17521 acccacaaca cccatatact cagcaactta taaattcatt gctataaata tcgtttgtaa
    17581 ataagcaaaa tgggggtttt taataaaatg aaagaaacta tacttgaagt aaataatttt
    17641 agtaaagact ttgagttaca tgcattgaac aagactataa cggcatgtag taatataagt
    17701 tttgaagttt caaaaggtga gtttttagga ataataggta aatctggagc aggtaagtct
    17761 accatattaa aaagtatcta taaaacatat ataccaacaa ctggagaaat aatttttaat
    17821 tcagaaatat atggtaatgt agacctggct aaaattggag accgagaaat gataaatctt
    17881 agaaagaaag aaataggata tgtatcacag tttttaaaga cacttccacg aataactgca
    17941 attgaactgg tagtacattc tttaattgag agtgggtttg aaaaagattg ttcgtatgat
    18001 atggcaaaag atatacttaa ccaatttgaa ataaaggaaa atttatggga tgcgtatcca
    18061 aataattttt ctggtggaga aaaattaaga ttaaatttag ctcaagctat ggttaaaaag
    18121 cctagacttt tgttacttga tgaaccaact gcatcacttg ataatcaatc taaaatatat
    18181 gtaaaagaaa aaatgttgga acttaaaagt caaggaacta caatgatagg aatttttcat
    18241 gatatagagt ttatggaaac tgttatagat aagaccttta ctatggcaaa agggactata
    18301 agtgaaagtg gggtagccta gatgataaaa ctaattcata atgcaaatat tgtattagaa
    18361 gatatgattt tagaaaatgg tcatttaatc atagagggag atacaataaa aaaagtatca
    18421 aatgatagga ttgattacgc atttgacttt aatgagatag atgaaataat agatgcaaaa
    18481 aacttatatg taatacctgg tattatagat attcacagtg atgccattga aaaggaaata
    18541 gagccaagac caagtacatt attaccattt aatatggcat tttatgaact agataaaaaa
    18601 ctacctgcaa atggaataac tacagtttat cattctatat ctttagggga tggagtagga
    18661 gttagaagta ttgataactc tttaaagatg attaaaaata ttgattcata taaaaatata
    18721 gatagtaaga gtataaatca taaagttcat ttaagatatg aagtgttgta ttatgaaggt
    18781 ttagaaaagg ttttagaact attagatgaa aataaaatag actacttatc aataatggac
    18841 cattctccag gacaaggtca gtatacaaat ccaacatttt ataaagagta tgctactaaa
    18901 gtgtggggag ttacagaaaa ttatgtagat acatggttag atgatttagt aaatctgcat
    18961 gataatttag attggaataa aatagctaat attataggta tagccaaaac taaaaatata
    19021 aatgttgcat cacatgatga tgatacatta gaaaaaatgg atttcataaa aaatataggc
    19081 ataaatgtat ctgaatttcc tataactctt gaagttgcaa aacattcaaa aaaattgggt
    19141 gttctaactt gtttaggtgc accgaatatt gtaagaggta aatctcataa taacaattta
    19201 aaagcaatgg atgctatatt ggaagattgt tgtgacattg tgtgttcgga ttatcttcca
    19261 tcagctatga taaagtctat gtgtatagtt gcagaaagaa taaataattt aaataaagca
    19321 gtcagtttat ttacctcaaa tcctgctaaa gcagttggta tatatgatga aagaggtagt
    19381 ataaaagaaa ataaaaaagc agatttagtt ttagtagata ttgatagtga atatccaaag
    19441 gttttaaata ctattgtaaa tggaaaaaca gtttataaaa gggaggtata aaaattgcta
    19501 acagagttac attgtcatac aagtatatca gataataatt ttactactga agaaataata
    19561 aaaaaagcag cagaaaagaa tataaatatc ttagcaataa caaatcatga cacattatct
    19621 ggattggatg aagctcttgt gcttggagag cattatggaa taacaataat accaggtata
    19681 gaaatttcag cctatgatta taaaaataga aaaagagttc atattcttgg atataacata
    19741 gatttaaaca gtcaagaaat aaaaaatttg tgcaatccaa tggtatgtga cagacataaa
    19801 gcatctgtta agatggtaaa taaaattata gatttaggat acaaaatatc tttggaagat
    19861 gtaaagaaat attcatcaaa gactggaata tttaaacaac atataatgaa agctcttata
    19921 gataaaggat atacagataa tatatattca agtctatata aagaattgtt tcatagagga
    19981 aacggcaagg tatatgtatc tttaaaatat gtggattaca gagatgcaat aaaagctata
    20041 aaaaattctg gtggaatttg tgtgttagct cacccgggtc aaatggataa ttttagcgct
    20101 attgaagaga tggtaaaagt aggattagat ggaatagaag tatatcatcc atctcacaac
    20161 aaagaactag aaaaagagtc gctttcttat gctaagaagt ataatctagt ggtcacagga
    20221 ggctcagatt accacggatt ttattctcaa cataattatg aacttggaag caagagctta
    20281 aataaagaag atttatttaa gtttaggtta gctttaggag gggtattata aatgtctttt
    20341 ttacaagtaa aaaatgtagg taaaagttat ggacaagtta aggtgttaaa agacatatct
    20401 attgatattg aaaaaggaga gtttatttgt ttactaggtc caagtggttg tggaaaaagt
    20461 acactgctta gaataattgc agggcttgag gataaacatg gtggaaaaat aataataaat
    20521 gataaggata tgactaattc accacctgaa agtagaaatt ttggtatagt ttttcaatca
    20581 tatgcattat ttccaaatat gaatgtttac aaaaacatag cctttggact agaaaataaa
    20641 aacataagta aatctaatat tgataaaaaa gttaaagaag ttctagacgt tgtagaatta
    20701 agtggatatg agaaaaaata tccatcacaa ttatcaggtg gacaacaaca aagggttgca
    20761 ttagctcgtg cgattgcttt agagccagac tttttacttc ttgatgaacc tttatctgca
    20821 ttagatgcaa aagtaagact taaacttagg gagcagatta gaagtttaca tagaaaacta
    20881 ggcattacaa ctataatggt aacgcatgac caagaagaag ctctttgttt agctgataag
    20941 atggttgtta tgaatagagg agaaataata caagttggaa cgccaaagga ggtatataaa
    21001 aatccagaaa caccttttgt agctgatttt ataggaacaa taaattttat agatgatggt
    21061 attaataaga tagctattag accagaagat ataaaagtag agtctaatag agattctaaa
    21121 gataaggata taaaagtggg agaaatttta gatatcgaat ttagaggatt taactataga
    21181 attacagttg agtatcgttc taagcaaatg aaattagatg tagtttccaa agttgcagaa
    21241 caaatgaaac tgtgcatagg ttctaaaata aactttaaga tacctaaaga aggaattgta
    21301 caatataaat cagagggatg tgcttaatag ctaggggtga ataaatgata acatcaaaaa
    21361 ataaaagaag aataagtgat agcacaatac aaaaatattt cttgattttt tcggtaattg
    21421 tactaatagt atttatatta tttccattag tatcattatt aaaaaatgca tttatggata
    21481 caagtggaaa ttttatagga atttcaaact ttagtaaata tgttgaaaat ccaagtctta
    21541 tagcatcatt taaaaattca atttttgtat ctacagtctc atctttaata tcactatgtt
    21601 tagcatttat atattcatac gcaataacaa gaacaaatat aaagtttaaa gattttttta
    21661 aaactatggg aatgtttcca ctttttgcac caactatgtt atatggtatt tcacttatat
    21721 acctttttgg aaataaggga atctttacaa ccatgggtct ggaagtacca ctatatggtc
    21781 cattaggaat aattatttct cagattctat ttacatttcc acaagctttt ttaatattat
    21841 ctgtagcact ttcgatggct gattatagac tttatgaagc agcagatagc ttgggtgcta
    21901 gtgaatttaa gaaattttgc aaaataacaa ttcctggtat aaagtactca ttacttagtt
    21961 cattctttgt atcctttata cttgcattta cagattttgg agcagctaag gtagtcggag
    22021 gaaactacaa tgtactggct actgacatat ataaacaagt agttggacaa tttaacatac
    22081 caatgggagc tactgtttct atggtaatgt taattcctgt actaatagca tttacattag
    22141 ataagatggc aagtaagaaa caaggtatga ccataactgc aaagtctatg ccatataaga
    22201 ttaaggaaaa taagtttaga gatagagtat ttttaatagt atgtagtttt ataacattgc
    22261 taatttttat tttaatattc gtttcagtta taggttcagt tataaagtta tggccatata
    22321 atttaagctt tacaatggaa cactatacat ttaaaggtat tttgggaagt ggaataaaaa
    22381 catacttaag cagtttgaaa atatcaactt taacagcaat atttggaact atatttgtat
    22441 tttttagtgc ttatataata gaaaaggtgg ataagtttcc taaattaaga caaacagctt
    22501 accttttatc aatgatacca atggccttac ctggcttagt tttaggtata tcatatataa
    22561 ctttttttaa ctcagcaagt aatccattaa attttttata tggaagtaca gcaatattag
    22621 ttatagtaaa tgtagttcat ttttattcag tttgttttat aacctcaaat tcatctttaa
    22681 aaatgttgga taaagagtat gaattagttg caaaatctat aaatattcca ttctataaag
    22741 tattttttaa tataacagtt ccgatgtcta taacatcaat acttgaaata gtagtatatt
    22801 attttgtaaa ttcaatggtt actgtttcag cattaatatt tttatatata cctcaaactc
    22861 agactgcatc aatatcaata ctaaagttag atgagatagg atatatagga ccatcagctg
    22921 ctatggcagt attagtgcta ttaacaaata taatagtaag acttttgtac gaatttgtaa
    22981 caaagaaatt aaaaaataac acacaaaaat ggcagcaaaa agatgttgca taggggagga
    23041 aaaaatgaaa tttaaaaagc tagtttcttt aggagtatta tcaacacttt tattaagtgt
    23101 aataacagga tgtagtactg attcatcagg ttcagattca aagtctgata gtaagacaaa
    23161 aggggattta acagtttaca cagcaattga agaagatagt atagagccat atttagctac
    23221 atttaaagag aaatatccag atattaaatt aaatattgtt agggcatcaa caggagatat
    23281 aacagcaaga ttattagcag aaaaggataa tcctcaagca gatgttgtat ggggagttgc
    23341 agccacaagc ttattggttg cagatgacca ggggatgtta gaaccatatg ctccaaaagg
    23401 ttctgaagaa atagagtctg catttaaaga tgataaagaa gttccttctt gggttgggat
    23461 agatgcttgg atgacaggta taacagtaaa cactaaagaa ttaagtgata agaacatccc
    23521 agttcctagt tcttacgaag atttaataaa gccagaatat aaaggtctta tatctatgcc
    23581 taatccatct tcttctggta cagggtactt aacagtatca gcccttatac aaattatggg
    23641 ggaagaaaaa gcatggcagt atatggataa gttgcatgag aatattggtg tatacactca
    23701 atctggttca gctccagcta catcagcagc ttcaggagaa tatcctattg gtatatcttt
    23761 tggatataga ggtataaaat taaaagagga aggctatcca gtagaagtag tatttccaaa
    23821 agaaggttct ggatgggata tagaagcaaa tgctcttgtg aaaaaagata atataaaaga
    23881 agcatcaaaa gtatttttag attgggctat aagtaaagat gcaatgaatg aatactctaa
    23941 gaactatgca gttactacta tttctacagg aaatccaata ccagaaggat tccctaaaaa
    24001 gccattagag caaatgatag ataatgattt aaaatcagca gcaaaaaata gagaagatat
    24061 actaaataaa tggatatcaa aatatgatgg taagacagaa aaagagagtt aagtaaaact
    24121 atacaaatgg agtaaaaaga aaaaattttt ttaagtagat tttattataa taatataagt
    24181 tgagaataat ataggtcaag aaaagtgaat attctataaa aatgtataga gtaagtatac
    24241 cattcagttt gaatggtata ttttttattt ttagagatta atttagaaat agatatacta
    24301 cagtttaaaa tagtatattt taatttcatt attaaagtat aga
//
LOCUS       DACTGW010000041        24152 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.041, whole genome shotgun sequence.
ACCESSION   DACTGW010000041 DACTGW010000000
VERSION     DACTGW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.041"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(33..755)
                     /locus_tag="I9573_001276"
     CDS             complement(33..755)
                     /locus_tag="I9573_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4795903.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECIGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            1125..2573
                     /locus_tag="I9573_001277"
     CDS             1125..2573
                     /locus_tag="I9573_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAT4795904.1"
                     /translation="MKRFLNKMMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVP
                     AISNISNIVFSNVDYLFLLGAVSAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNA
                     GVFGGIIVGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIY
                     PQAAMNQFGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVI
                     RGEIPRFMAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALT
                     SFMATVTEPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSF
                     SYGDKAWLVIPVGVVFFVLYYVTFKYIIIKDNVQTPGHEVEIEIGDKVSEKEKNLKLS
                     HGNFSYMAKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLS
                     NTSVQIIIGTDVVKVKDEMEMQLDELRKQADA"
     gene            2566..3345
                     /locus_tag="I9573_001278"
     CDS             2566..3345
                     /locus_tag="I9573_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HAT4795905.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            3608..4333
                     /locus_tag="I9573_001279"
     CDS             3608..4333
                     /locus_tag="I9573_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT4795906.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            4463..6388
                     /gene="mngA"
                     /locus_tag="I9573_001280"
     CDS             4463..6388
                     /gene="mngA"
                     /locus_tag="I9573_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAT4795907.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDSNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIVLAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKKADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVIGKPVAA
                     LNNGLINWLNGMTGINAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIHVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            6532..9147
                     /gene="mngB"
                     /locus_tag="I9573_001281"
     CDS             6532..9147
                     /gene="mngB"
                     /locus_tag="I9573_001281"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAT4795908.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNKDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YNRNEIVNAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKISDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIINVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGKKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKIPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANVVFNKNIKTAKWTNLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            9429..10583
                     /locus_tag="I9573_001282"
     CDS             9429..10583
                     /locus_tag="I9573_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAT4795909.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(10706..12619)
                     /locus_tag="I9573_001283"
     CDS             complement(10706..12619)
                     /locus_tag="I9573_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAT4795910.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     STGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNLTLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYKNKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            12867..13184
                     /locus_tag="I9573_001284"
     CDS             12867..13184
                     /locus_tag="I9573_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4795911.1"
                     /translation="MKNIKVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLPEIDCLLLGPHLDYMKDEIQKKADEYNVPVACISKTAYGRLDGVKALEEALELI
                     LNK"
     gene            13192..14439
                     /locus_tag="I9573_001285"
     CDS             13192..14439
                     /locus_tag="I9573_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT4795912.1"
                     /translation="MGRLFMNKFIDLLSEKFTPVVNKITKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVALVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDSMLPIAVIMFIGWGLIYGLNFDMFEIIVRIFKPVSDIAQTLPGFV
                     LLNFLVVFLYSLGISGWVLSPITYAIQLSAIAANAAAVASGGLATNIYTYEVVQTGWI
                     TLGGWGATLPLVLMMCLSRAGRIKAIGRACIVPSIMNINEPVMFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYLPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEELKLESQEA"
     gene            14468..14821
                     /locus_tag="I9573_001286"
     CDS             14468..14821
                     /locus_tag="I9573_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAT4795913.1"
                     /translation="MTTEEHVIDEELVEVAMQIILRAGEARTEIKHALNDLERFDYKN
                     ADLKLAKAKEFMTEAHRAQTNIIQGEASGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            14852..16300
                     /locus_tag="I9573_001287"
     CDS             14852..16300
                     /locus_tag="I9573_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAT4795914.1"
                     /translation="MSKFPKDFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKDRDI
                     SKHYESDMTVDKIKFAMKDNNGYYPKRNGIDFYHTYKEDLKLLKELGLKCFRFSINWA
                     RIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSNRKV
                     VDFFVRYAELILNRYKDKVKYWIVINQINLMYHESFNSVAFCVDQVDDVEEAKYQAIH
                     HQFMATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRIQYFFTDVS
                     FRGEYPAYIKRFFKENNINIVMEEGDDKVLKENTMDFLALSYYYSTTVSASKNTMNPI
                     DNVKNQYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKVHDS
                     YRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDNNGN
                     GSKERYKKDSFYWYKNVIRTNGEELKEKVDSF"
     gene            16388..17005
                     /locus_tag="I9573_001288"
     CDS             16388..17005
                     /locus_tag="I9573_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795915.1"
                     /translation="MNVIKKFIFVILIINILGCGISLILQSSLGSDAITLLNEGIHIK
                     LGISYTIAGLIYNGTLLLIALLLNKKSLGLGSCVYVLVAGIFIDLYIYLLLDFNISSL
                     GIIIRIMSFIIGHILMCSGFAMLIKFDIGMSPLDAILIYIEGKFKYPYKVLKTIADLI
                     FLILGVYLGGNLGFGTIFSILCTGTTISIVSKMISVNKKRYYSSI"
     gene            17302..18054
                     /locus_tag="I9573_001289"
     CDS             17302..18054
                     /locus_tag="I9573_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT4795916.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            18156..19076
                     /locus_tag="I9573_001290"
     CDS             18156..19076
                     /locus_tag="I9573_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAT4795917.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            19079..20995
                     /locus_tag="I9573_001291"
     CDS             19079..20995
                     /locus_tag="I9573_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAT4795918.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDGHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMISATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            21015..21884
                     /locus_tag="I9573_001292"
     CDS             21015..21884
                     /locus_tag="I9573_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAT4795919.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIGTVKC"
     gene            22394..23857
                     /locus_tag="I9573_001293"
     CDS             22394..23857
                     /locus_tag="I9573_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HAT4795920.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVKVLEKEIVDGLEELERMLEV"
     gene            23861..>24152
                     /locus_tag="I9573_001294"
     CDS             23861..>24152
                     /locus_tag="I9573_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAT4795921.1"
                     /translation="MVDEAKILDVVSISRENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKE"
BASE COUNT         9311 a         2622 c         4426 g         7793 t
ORIGIN      
        1 aaaaaaatta tgtttaatta aacataattt ttttaatatc tttgagtaac atccatgaat
       61 acaaatgttt caggtgtatg ctttgattct gtatattcaa agagctttcc atcatcagta
      121 tatacactat ttttaataac accaacacaa ttataatcat ttagaggtag cgtttttaac
      181 tcaagctctg tagctttttc aattttaaat atctttctgg atgaaacaat ttttgtacct
      241 atacactctt ctatatattt ataaatagag ccttgtgcta tagaaacatc aaggtcttta
      301 actatactac atagaaaata gttaatgtcg agaattttag gtatgttatc aatatatctt
      361 agtctgtgaa ggtaatatac ttcactacca actttaaaac ctgtcttttt agataactta
      421 ttatcaatta agattttgct gaagtggaga acactagtag cagtattaac ctttttatct
      481 tctacaatcg attctacacc tttgaaattg tgcatattta gaaaaaaatc attagaacaa
      541 ctattttcta acactacaac acccttgcct tttatacttt gaacatatcc ttctgtgttt
      601 aattgagaaa ttgctctacg tacagtgttt ctgctacatg aaaacctttc aataagttga
      661 agttcagtag gcagcatcat attacttgta tatttaccat caagtatttc ctctttcaat
      721 acttcatata tctctgaaaa tttattttta gccatatacc cctatccctc cttaaaggtc
      781 tttagtaata ttattgaact gatatgaaca aattatatca aatgtgttta gacatgtata
      841 gtgaaaatag aattatataa accataaaaa ggataaattt gaactatttt gtgggttgtt
      901 tcaaatacat ggttaaacat attttaaaaa taaaaaaaat aaatttaaaa aataggctag
      961 atatagcaat atacttaaaa ggatttagat atgaaactaa atgataaaaa taaaatgaat
     1021 aaaaaatatt gacaatttgt ttaaacaaat ataatattta aaatataaaa tgtgaataaa
     1081 tcgttttcac aaaaaattaa accgaaatag aaaggggatt ggatatgaag aggttcttaa
     1141 acaaaatgat gcaacaactg tcacttttag gaaagactat gctggtgcca atagctgtaa
     1201 tgccagcagc aggtatatta ggattattat ttggtccaaa tatgcttaat gtgccagcta
     1261 tatctaatat atctaatata gtattttcaa atgtggacta cttattttta ttaggagcag
     1321 ttagtgctta ttcaaagtct aaagacaaga caagtgtaat ggttgcagcc gtagttgcat
     1381 atatgatatt taagtcaaat ttagagttat tgaatgatac attaaatgca ggtgtatttg
     1441 gtgggataat tgttggagct tgtatagcat ggacttacaa taaaacatat aaagttaaag
     1501 ttccagcatt tttaagcttc tttgcaggag aaaaatgtgt aattacacta tcaccagtgg
     1561 ttgctttaat tttatcatat atatttagtt tattatggat atatccacaa gctgcaatga
     1621 atcaatttgg tttttggttg ggaggagctg gtgcaatagg aatattcttg tttttattct
     1681 gtaaccgtgc actaatacca actgggttac atcaagtttt aaatgcttac atattatttg
     1741 aaatgggtga atatacaaca tcaacaggag aagttataag aggagaaatt cctagattta
     1801 tggcaggaga cccaaatgca ggattcttct ggacaggatt ttacataatt atgatgtttt
     1861 caatacctgc tatttcttat gctatatata aaacagcaca agataaaaag aagcaagaag
     1921 taaaaggtgc aatgaccgct ggagcattga cttcttttat ggcaacagta actgaaccaa
     1981 tagaattttc gtttttattt gcatctccaa agttatatat aattcattct ttttattctg
     2041 gtctaggtgg agttgtacta tatttactgg gtgctcgctt gggttcattt aatggagcaa
     2101 gtataattga ctatgtctta agttttagtt atggagataa agcgtggtta gttatacctg
     2161 taggagttgt attctttgta ttatattatg taacatttaa atatataata ataaaagata
     2221 atgtacaaac accaggacat gaagttgaga tagagatagg tgataaagtt agtgaaaaag
     2281 aaaagaattt aaaactttct catggtaatt tttcatatat ggctaagcag ataattaaaa
     2341 attgtggtgg ttatgaaaat atagtgactc ttaataattg tatgacgagg ttacgtttag
     2401 aagtgaagga tgccactatt ttgaatgatg ataatattaa aaaaacagga gccaaagggg
     2461 taattaaatt gagtaataca tcagttcaaa tcatcatagg aacggatgtt gttaaagtaa
     2521 aggatgagat ggaaatgcaa cttgatgagt tgagaaagca ggcagatgca taaaatgaag
     2581 aaattagtag ttagggcaga tgatatagga tattcagaag gtgttacact agggataata
     2641 tcttcttata agaacggtat tgttacaagt gctggaatca tggtgaatat gcctttttca
     2701 agacaagcta tagaaatggt aaaaaatgat gatgtatgtt ttggtttaca tgttaattta
     2761 gtaataggag aaccttgttg taaagaacat attaagataa agaatttgtt agatgaaggt
     2821 ggaaaatttg tttctagcaa aataagaaga acgcaactac tgacaggtaa agaggttttt
     2881 aattatgaag aagtatatga agaagtttgt gctcaaattg aaacttacaa gttgtatatg
     2941 agtaaattgc ctgagtatat tgatatacat ggtatagaag aagatgtatc aatatcagca
     3001 atgtgtgatg ctgcaaaaga atacggtata ccaagttgtc cctattattt agattcatgt
     3061 attagcatgc caagtaaata tcctcagaat gatttttaca agttaaataa atcttttgtt
     3121 aaattatttg aagaaaaata tataaattta gataaagata taacattgat ggttactcat
     3181 ccaggattta tagattatag tcttataaaa tcatcttctc ttattcaaga gagagtatat
     3241 gaacatgcaa tgctatgtga taaggaagtt aaaatgtggt taaaaagaaa taatattgaa
     3301 ttggttgatt ttaaagatat tttcaagtct aataattata tataaagaat agattaccta
     3361 aagttttaat taaaaataag tatttgaaaa aaatgtgtat tttctaaaaa aatattgata
     3421 gagatgtatc aaaataaaaa cctgaatcat ctctatcaat ataggaagta cacatttttt
     3481 atgtataata aaaagagtag tatgcattaa taaattaaaa atattaaatt ataattatat
     3541 taacgaaaat tttagctaat atttttgaat tttacttatg aataataaaa tagatggagg
     3601 agaaactatg gaattttatg aaagagcaaa aaaatatgag tataaattta cagatacaga
     3661 agacatgata attgagtata tgagaaaaaa caaagaagaa atagtaaaat tatcaataca
     3721 atctttagca gataaatttt ttactgtccc aaataccata gttaggctat gtaaaaaaat
     3781 ggagtatgat ggtttctctc aactaaaaaa caatttaaaa gaagagttaa aaaatgagga
     3841 taaagagcca aaaaatggtt ttgaatcaga tataaaaaag acattagagc taatagatta
     3901 tgaaaaaata aataaagtag cagatataat tcaaaaatct aggagtatta tagtttatgg
     3961 aataggtcaa aacattcctt tatgtgagat attagtaaaa gaaattagag gtgggcatga
     4021 taatataact tactatagcc aaagacatga agtacttcat gatataggaa aattaggtga
     4081 acaagatatg tttttcgcgc ttagtttaag tggagaaacg aaagaagtta tagaagcagc
     4141 aaagacagca gttaataagg gttgtaaggt agtagctcta acacatatat gtgaaaatca
     4201 attgcaacaa attgcagata taagtttata ttgctattca acaattaaac aagtaaatgg
     4261 gtacaatata tcagataagg ttccaattat gatgattatg agagcaataa tagaacaata
     4321 ctggttaaga tagagatttg tgtataaata cacaaatcct tattttttaa gaagaaattg
     4381 tgtgtataat ttcaaaaaaa cattttcatg atttatttta tgctatattc ataacattaa
     4441 atgatttaag gaggtcgtaa ttttggaatt taagaaaatc acaaatgaaa accttgtttt
     4501 tttaaaacaa aaaataaaaa caaaggaaga cgcaataaag ttcttagcaa gaaagctata
     4561 tgatgaaggc aagttaacat cttatgagga atatttagaa gctgtaatgt acagagaaac
     4621 tttatctcca acaggttttg aaagagggct tgcaattcct catggaaaat ctaagtcagt
     4681 taaagaagct gcatttgcag ctatgacctt agagacacca atagaaaatt gggaaagcgt
     4741 agactcaaat aataaagtag agctaatatt tttaatagca gtaccagaaa gtgaaggtgg
     4801 aaatacacac atacaaatac tttctgagtt agttactaga ctgtctaatg aagattatat
     4861 gaatatgttg ttaaattcaa aaactaagaa agagttattt aataacttag atataaaaga
     4921 aaagaaagaa caaataaaag aagagattag gattcaaaat gaatcaaaaa aaattgtatt
     4981 agcaataaca gcatgccctg caggtatagc acatacatat atgtcagcag aagcattaat
     5041 taaagctggc aaagaaatag gtgtggatgt gtatgtagaa aagcaaggag ctaatggtat
     5101 ggtggaccct catacaccag atataataaa aaaggcagat gcaataatat atgcaacaga
     5161 tgtagcacct aaaaatacag aaagatttga acatttgcca aacataaaaa cgtcagtagc
     5221 agctccacta aaaagggcaa aagaaataat atacgaagct ttagaagtag cacaaaaaca
     5281 aggtaaaggt gattatatag aaaaatcatc tgatatatca tattgtgaaa aatcatcatg
     5341 gaaaaaagat gtaaaatcag caatacttac aggagtatct catataatac cacttattgt
     5401 ggcaggaggt atgatacttg catttgcagt attagcatct caagcatttg gtttacaaga
     5461 actatacaat caagaaggtt cttggttatg gcaattaaga aagctttctg gagggatgtt
     5521 aggtacattg atggttccag tattatcagc atatatgtct tactcactag ctgaaaaacc
     5581 aggtcttgga ccaggatttg cagcaggtct agcagctgat ttaataggta gtggatttct
     5641 tggaggtatg ttaggaggtt tacttgctgg ttatatagta aaatttttaa agaagaatat
     5701 accagccaaa ggcacattag ctggattcgt aagtttttgg gtatatccag tagttggttc
     5761 tttggtagta ggtttattga tgttatttgt aataggaaag cctgtagcag cacttaacaa
     5821 tggtcttata aattggttaa atgggatgac tggaataaat gcaatactac taggtgcaat
     5881 aataggagct atggtttcat ttgacttagg tggaccaata aataaagcag cctatacatt
     5941 tgcaatagga gcaatagcaa gtggaaattt tataccttat gcaatatttg catcagttaa
     6001 gatggtctca gcttttggag ttacaatagc tactgtttta ggcagagatt tattttcaga
     6061 atcagaaaag gaaataggaa agcaaacttg gatattagga ttggctggaa taactgaagg
     6121 tgcaatacca tttatgatag aggacccaat ccatgttata ccatcattag tagctggttc
     6181 agccataact gggtctatag tagcattctt caatataggt cttcaagtgc ctggagcggg
     6241 gatattctca ttggcgcttt tagagggaag tatgtcagga tttattatag catcaatatg
     6301 gttctttgcg gcagtacttg gagctataat atcagcaata ttattaataa taactaggaa
     6361 acaaaaaaat caaagaaaga tacattaaaa gatacgttat tagatattta aatcaaaggt
     6421 ttaattatag tgataaaata aatattaaaa aattgtgtaa actcagtagt atttttattg
     6481 gatttttaat tagacagaaa atattatcag ataagttttg gaggataagc aatgaaaaaa
     6541 aatgtacaca tagttcctca tatgcattgg gatagagaat ggtacttctc aactgaggaa
     6601 tctagaatat tacttgtaaa taatatggaa gaaataatgg atatgttaga aaataatcat
     6661 aactatccat attatgtgat ggatggtcaa actgctatat tagaagatta tttagcagtt
     6721 aagcctgaat gtaaggagag aataaaaaaa ttagttcaag aaggaaggct aataataggt
     6781 ccttggtata ctcaaactga tgaaatggta gttggtggag aatccatact tagaaattta
     6841 ctgtatggaa taaaagattg tgatgaattt ggagaatata tgaaaatagg atatttacca
     6901 gattcatttg gtcaaagtgc tcaaatgccg caaatactaa atgggtttga tataaaatac
     6961 tcaatgtttt ggagaggatg ttctgaaaga aaaggaacta ataaaacaga gtttaattgg
     7021 aagagtgatg atgggtcttc tgttttggtt caaatacttc cattaggtta tgcaatagga
     7081 aaatatctac caatgaatga agatgagcta agaacaagaa tggataaata tttaccggtt
     7141 cttgataagg gtgcaactac tgaccatata atacttccaa atggacatga ccaaatgcca
     7201 attcaaaaga atatatttga agttatatat aaattaaaag aatgttatcc agagagaaaa
     7261 tttttcttaa gtagatatga aaacatattt aaagagctag aaaaaaataa ggatatagat
     7321 acaataaaag gggaattttt agatgggaaa tatatgaggg ttcatagaag tatattctca
     7381 acaagaatgg atataaaatc agcaaatgct agaatagaat caaagataac caacatattg
     7441 gagccactag cttctattgc atattctcta ggatttgaat atcatcatgg attaatagag
     7501 ttaatttgga aagaaataat gaaaaatcat gctcatgaca gcattggatg ttgttgttca
     7561 gataaagttc accaggaaat aatgaataga tttttcttag ctgaggaaaa agtagaccaa
     7621 ttaataacat tttacaaaag aagaatagtt gatgcaatga gttgtgaaat ggctttagat
     7681 aagttagtag catttaactt aatgccttac aataggaatg aaatagtgaa cgcccaagtt
     7741 ataactaaaa tgaaagcatt tgaaatattt gataaagata ataataagtt ggattttgaa
     7801 gtggtacata aagaagtaat agatgcagga ttaatagacc gtcaaattgt tcattatgga
     7861 aactatgatt catttatggt atatacaata aactttaaag ataaaatacc tgcaatgggg
     7921 tataaggcgt atatgataaa agaagttgaa catatgttag aaaaagctta tgaagtatgt
     7981 gacatggtag ataatgactt ttatactatt caagttaatg aaaacgggac attaaagata
     8041 tctgataaaa aattaaataa aacttttgaa aatgtattat tgatggaaaa tggaggagat
     8101 gaaggagatg agtatgattt ttcaccactt ccaaatgaga aattaatatt taacgataat
     8161 atagtagcaa attccactat aaagaaaaat agatttaata atgagataaa gataaattat
     8221 agattaaaag taccaaaaga tatacaagct agaaaaaata atagagcaga ttcatttatt
     8281 gactttgata tagttataaa tgttccaaat gataaagcta taataaatgt agtattcaat
     8341 ataaataacc aagcaagaga ttgtagggtt agaacttata taccaacaaa tatagcttca
     8401 aagttttctg tgtctgataa tcagtttgga catataaaga gaagtgtgta tgatgatgct
     8461 atggaagttt ggcaacagga agaatggagt gaaagaccag attctatata tccaatgcta
     8521 acattcgttg gattgtctga caaagagcat ggagtagctg ttcttacaaa cagtacaaga
     8581 gagtttgaaa tagtagggaa aaaatttgac acaatagcta taacattatt tagaagtgta
     8641 ggattcctag gtaaagaaga aatggcaaga cgcccaggtc gtccatcagg aataaagttg
     8701 cctactccag attctcaaat gataggagat ataaaaatag acttagcaat agtcactcat
     8761 gaaaaatcta cactagaagc aaatgttgca aatatggcta aagagtattt aacaaaaata
     8821 caaacttata ataaaatacc atataatgct atgaaattga atcaatcaga aatagaagta
     8881 gattattcat atagtttact taaggaaact aataaggacc ttgtgttaag tgttgtaaaa
     8941 aaggctgaaa aagaagaggc attagtagtt agattttaca attctaaaga agaaacaaaa
     9001 tcagctaacg tagtatttaa caaaaatatt aaaacagcta aatggactaa cctaaatgaa
     9061 aaagaattag aatctatatc aatagataat aactctgtaa atgtagagtg cctaatgaac
     9121 caagttaaaa ctatattggt taaatagtgc taaaaatgat tagatagtgc taaaaaggaa
     9181 ttatcttaaa atagagataa tcctccttat cattgttgaa ataataaaca tatatttaat
     9241 tttatacaaa aagagtgtct catgaactaa aaagttcatt ttgagacact ccctttttta
     9301 taagataaaa gaatattaag ggtataagtt gtatacaaag tgaaatatag agtataaaat
     9361 aaaagataaa ttaagcttat aaaattatat atttaaaata aagagtaaat aaaaaggagt
     9421 agataaatat gaaaatatta atttctatag attctttgaa aggaagttta tcttcaatag
     9481 aggcaggaaa tgcaataaaa aaggggatat tgaaggttaa agaagatgcg caagtaaaga
     9541 tattgccact tgctgatggt ggtgaaggga cagtagatgc tttagttcaa gggatgaatg
     9601 gtaaaaaaga gactatagaa gttactggac caatagctaa aaaagtggat gcaacatatg
     9661 gattattaaa aaatacaagt acagcaataa tagaaatagc tcaagcatca ggattaacat
     9721 tggttccgac tgaacttaga aatcctttat acactacaac ttatggtgtt ggggaaataa
     9781 taaaggaagc aataaataaa ggctatagaa attttatagt tggaataggt ggaagtgcta
     9841 ctaatgatgc tgggataggt atgcttcaag ccttagggtt tgagttttat gatgaaaata
     9901 acaagttagt aggattaggt ggaaaagttt taaatgaaat aagacatata aaaatagaaa
     9961 atagattaaa ggaattagat gaatgtaagt ttaaaatagc ctgtgatgta aataatccgc
    10021 tgtttgggaa aaatggagct gcatacatat atgggtctca aaaaggtgca acatcagaaa
    10081 taattgagga gttagataat gggcttagaa atttttccaa agtagttaaa aattacttaa
    10141 gtaaagatgt agctaatgta gaaggagcag gagcagcagg aggattaggt tttgcttttt
    10201 tagcattttt aaattcaaaa ttagaatctg gaataaaaat aatattagaa gaaattaaat
    10261 tagaggaaga gttaaaagat gcagattttg tcataactgg tgaaggtaga ttggataatc
    10321 aaactgctat gggcaaggct ccaataggag ttgcaaaact ggcaaaaaaa tatggggtaa
    10381 aagtaatagg tttggctgga gccacaacag aagatgcagt taaatgtaat gaagagggta
    10441 tagatgctta tttttctata gtaaatagag ctatgacaat agaagaagca atggataaag
    10501 caactgctag tgagaatatg acagctacaa caacacaaat attcaatctt ataactagta
    10561 tacaacaatc aaaaaatatg tagtattaaa gcaaagtata ttaggtaaaa agctgtcaca
    10621 tattaattta gactttttag tgtgacagct tatctatttt gtgttaataa cttaagaaac
    10681 atatattgga aataaattaa tggatttaga tattttgaat aagataagct attctattca
    10741 taaaactact gtagttatct gccttagtaa gttctttaac attattccac tcagataata
    10801 tttcaataaa gctttcaaat atgtaattga atttttcttt ttctttttca tttataggta
    10861 tcatagctat aatttgaact ttttcctcac cccatttaac tggtttatcg ttaacaatta
    10921 aacatactcc agtttttaaa gcatccattt tcatgctatg aggaattgct acattgttat
    10981 taaatgcagt tgaagacatt ttttctctta taagaacatc atctatataa ttgggtttga
    11041 catatttatt tttgtacaga atatttccca taaattttat gtaatcttta gcactatcta
    11101 aatatatgtt tttcataaac aaagattcac taaagtacat ttctaaatga ttttgtacag
    11161 taagtatatt ttttctatct ttaatctgaa taaatttact ttggatatta tcataatctt
    11221 ttctgtttag gtaaggattt acataaacaa aaggtatagg gcatctatca ctaattggta
    11281 aagtagtaat aaccatgtct accttattgt caaaatcaag attatgagta tctgaaaaaa
    11341 tttgaataat ttctatggat tcattaaatc tcttctcgag gtctcttact attttgaatt
    11401 gtaaatcata gtaatttgga gtaattaatg cacagagaat tttatcttct agtaattttt
    11461 gtctatcaaa aaatgaacct acatgaagag atatatacga tatttcatct tcatctattt
    11521 ctatgttttc taaaatttga atttgattac aaatataaac agatatatca tatatcagag
    11581 ggtaggaatc ttttaacttc tgaggtattt gatttctcaa aacttgatta tttttagccc
    11641 tactgattaa attttttaca tgaagagtaa attttacaac aaattcatca tcagataaat
    11701 ctataaagta taagtcatat acatttttaa ttattttttt agttaattta acaaaatgtt
    11761 catctatata atttggcaat gacttagtat ccatttcatg ataatttaag actgtggttt
    11821 tgtttgttaa ctgaaatact aagtagtata attctgcact agttaaagtt aaattgtatt
    11881 cagactctaa aaaatttgat atatctcttg aaatgcttat atcattatta ttatctatgt
    11941 agtttactac ttcgacagat tctatgtagt tattcttttt tattctgtct atagttatca
    12001 ttaaatgaag aagtatgtta tttatagcgt actcatttat aaacaaattg tattttttta
    12061 atatatatat tatattttct ttcagtttgg tcaaattata ctcttgatat attttttgta
    12121 catcaaataa gcttaaaaaa tcacttcccg tttctttaaa tattatagag ctcattaatt
    12181 tgcgctttgc agactcattt ccaattagtt tcaataaaaa tttcgattgc tttatcttta
    12241 aattataact tcctataaat ttattagctt tgactatatc cccttcaata gtagctggac
    12301 taacaaacaa ttcttcactt aaatcaaata tattaattcc attactatta actataagtt
    12361 cttttaaaat taaatttaac ctgttatcag gtgatatatt ttctgaacta tcaaccttgg
    12421 tttgacagtt attattgaga tttactttat atcctttttt agaggataat ataaattcac
    12481 cagtagaatt aatttcattt acatattttc taatcgaacg tgttgataca ttaagataat
    12541 tagctaatgt ggatgaatct atccaatcat gttgcgtttt tatgaagttt aaaaataact
    12601 cttttttaga tgttttcatt agattccctc tcatagtttt aaaataaaat ctgtatgata
    12661 tgatatttat ataattgaat gtacgtatta taatgtacat tatactatat atttatgtgt
    12721 ctggttaaga gtaaatgatt tcctattatt aggaaatata tttatgtgaa aggaaattat
    12781 ttaggaagta caatataaat acaatcaaat caaataaaaa ttcattaaga aataaattaa
    12841 tgaataaaaa tactggaggt attgttatga agaatataaa agtgttgttg gcatgtggct
    12901 caggagcaag tacaggtttt atggcaacta gtatgagaaa gagtgcaaag aaaaaaggca
    12961 tagaagcaga tataaaagct agaagtgagt cagagataga taaatattta ccagaaatag
    13021 attgtttatt attaggacca catttagatt atatgaaaga tgaaatacaa aaaaaagctg
    13081 atgaatataa tgttccagta gcatgtatat ctaaaacagc atatggaaga ttagatggtg
    13141 taaaggcttt agaagaagca ttagaattaa tattaaacaa ataaaatatc tatggggaga
    13201 ttgtttatga ataaatttat agatttgtta agtgaaaagt ttactcctgt tgtaaataag
    13261 ataactaaga atatatgggt acaatcagtt caatcaacta taatgaaagt tttgcctatg
    13321 gtatttgtag gttctttagt aactatagtt tctgtcttga agaattacat aagtttctta
    13381 cctgatttaa gtccaataaa tcaatataca tttggacttt taggactatt tatagcattt
    13441 ttattaccta tggaaataat gaagaataag aaatttgaaa gtatgagtgt agtagctggt
    13501 cttgcaggtg ctggattaat gttaatgatg attcgtccag agattacgaa tgaaggagct
    13561 atatttaact tcaatagatt tggaggagaa ggaatgcttg tagcactagt tgcaggatta
    13621 ttctccggat taataatgtc tttatttggg tctttctcat tttttggtga agattcagca
    13681 cttcctgatt ttgtaagtaa gtggtttgat agtatgttac ctatagcagt aataatgttt
    13741 ataggttggg gactaattta tggacttaac tttgatatgt ttgaaataat agttaggata
    13801 tttaaaccag tatcagatat agcacaaaca ttgcctggat ttgtactatt aaacttctta
    13861 gtagtatttt tatattcttt aggtataagt ggatgggtat tatccccaat aacttatgca
    13921 attcagctta gtgcaattgc agctaatgca gcagctgtag catcaggagg gcttgcaact
    13981 aatatatata cttatgaagt tgttcaaaca ggttggataa ctttaggtgg gtggggagca
    14041 acattaccac tagtgctaat gatgtgttta tctagagctg gaagaataaa agcaatcgga
    14101 agagcctgta tcgttccttc tataatgaac ataaatgaac cagtaatgtt tggagctcca
    14161 gtagcatgga acccattttt aatgttacct atgtggatag gaacagtagt aatatcaata
    14221 actacttact tatcaatgag tatgggattt gtgacaattc cgagtaaggt gtttaattta
    14281 tggtatcttc cttttggtat atctactttt atggtaaatc aagatgttag agggcttatt
    14341 gttttagcaa taaatatagt attgctattc atgatatggt atccattttt taaagcttat
    14401 gaaaaagaag aactaaaatt agagagtcaa gaagcttaaa tttagatgtt gaaaggaaat
    14461 tataaatatg acaactgaag aacatgtaat agatgaagaa ttagtagaag tagcaatgca
    14521 aataatactt cgtgcaggtg aggctagaac agaaattaaa catgctctta atgatttaga
    14581 aaggtttgat tataaaaatg ctgatttaaa actagctaaa gcaaaagagt ttatgacaga
    14641 agcacataga gctcaaacta acattataca aggggaagct agtggagaaa aaagagcaca
    14701 ttcactttta tttgcacatg ctcaagatac tcttatgaca atattcagcg agttaaatat
    14761 aactatgtct ttagtaggaa tagtaaagtc tatagaatat aaggtaaata atagtaaatg
    14821 atttaaataa ataaaataag gagtgattaa attgagtaag tttcctaaag attttttgtg
    14881 gggtggagct gtagcagcta atcaattaga aggtgcttgg aatgaaggtg gcaaagggtg
    14941 gtgcttagct gatgtacata tgtatgataa agatagagat atatctaaac attacgagtc
    15001 tgatatgact gtagataaaa taaagtttgc tatgaaagat aataatgggt attatcctaa
    15061 gcgtaatgga atagactttt atcatacata caaagaagat ttaaagcttt tgaaagaact
    15121 agggttaaag tgttttagat tttcaataaa ttgggctaga atatttccaa atggagatga
    15181 tttagagcct aatgaagagg gattaaagtt ttatgataat cttataaatg aagtaataga
    15241 taatggtatg gaacctattg taacactact ccattatgag actcctttga atttagtatt
    15301 aaaatataaa ggatggtcta atagaaaagt agtggatttc tttgttagat atgcagaatt
    15361 aatattaaat agatacaaag ataaagttaa atattggata gttataaacc aaataaattt
    15421 aatgtatcat gaatcattta attcagttgc attttgtgta gaccaggtag atgatgtaga
    15481 agaggccaaa tatcaagcta ttcatcatca attcatggct acagctttaa ctaagaagct
    15541 tggaaaagaa ataaattcag atatgatgat agggatgatg atggcagatt gtacatctta
    15601 tccagaagat tgcgaccctg aaaatgtagt atttgcttta aaaagaaata gaatacaata
    15661 tttttttact gatgtaagtt ttagagggga gtatccagct tatattaaga gattcttcaa
    15721 agaaaataac ataaatatag ttatggaaga aggtgatgat aaagtgttaa aagagaatac
    15781 tatggatttt ctagctctat catactatta ctctacaaca gtatcagcaa gtaaaaatac
    15841 catgaatcca attgataatg taaaaaatca atacttaaaa gcaaatcctt gggggtgggc
    15901 aattgaccct aaaggattat acaatacttt atctcaatat tatgatagat atcaaaagcc
    15961 aataatgata gctgaaaatg gatttggaat gcatgataaa tttgaagatg gaaaagttca
    16021 tgatagttat agaatagatt atttaggaaa acatatagaa caaataggtg aagctatagc
    16081 tgatggaacc gaaatattag cttattgtat gtggtcgcca atagatatag taagttgttc
    16141 atctcaagaa atggaaaaaa gatatggatt tatatatgta gatatagata acaatggaaa
    16201 tggaagcaaa gaacgttaca aaaaagatag tttttattgg tataaaaatg taataagaac
    16261 taatggagaa gaattaaaag aaaaagtaga tagtttttaa atataaataa tccctcttat
    16321 aaatactatg ttattaatac agttttataa gagggattat aattataaat aaggtggttt
    16381 attaattatg aatgtcataa aaaaatttat atttgtaatt ttaattataa atatactggg
    16441 atgtggtata tctcttatac ttcagtcaag ccttggtagt gatgcaatta cacttttaaa
    16501 tgagggtata catataaagt taggtataag ttatacaata gcagggctaa tatataatgg
    16561 aactctatta cttatagcat tgttattgaa taaaaaaagt ttgggattag gaagctgtgt
    16621 ttatgtatta gtagcaggaa tatttataga tttgtatata tatttattat tagattttaa
    16681 tataagtagt ttagggatta tcattagaat aatgagcttt ataattggac atatacttat
    16741 gtgtagtgga tttgcaatgc ttataaaatt tgacattgga atgagccctt tagatgctat
    16801 attaatctat attgagggca aatttaagta tccatataaa gttttaaaaa ctatagcaga
    16861 tttaatattt ttaattttag gtgtatattt aggcggaaat ttagggtttg gaactatatt
    16921 ttcaatatta tgtactggaa ctacaatttc aattgttagt aagatgattt ctgttaataa
    16981 aaaaaggtat tactcaagta tataaattga taagtgagag tataggataa tgtttatgaa
    17041 tcattttcaa taattcgagt taatttgagg ctgtaagaat tggagttact cagtttcagt
    17101 aattgagtaa ctccaatttt tattacaaaa tactctaata attacataat taattgaagc
    17161 ataaaattat tatattataa atttacatat atagacattg actaaatctt aattgtatat
    17221 aaaaataagt agttaagagg taatatatta tattactcga taaatttaga caaataaaac
    17281 ttatataagg ggagataaca tatgataatt gagctaaaca aaagtaattc tgaaaatcta
    17341 acgaagacgg aacttgaaat aattaaattt ataaatgaaa atgaatcaat attaccagaa
    17401 ttatctatag tagagattgc tcatgaaaca tattcatctc cagcaaccgt ttcaagagca
    17461 attagaaaat gtggtttaaa tggattcaat gaacttaggt atagattaac agtaaaagat
    17521 gaaaatgatg atatacaaaa tatgggggat ataataaata aatcctttat agaagcacag
    17581 agagttatag aacaaatatc tttaactaca ctaatcaata taataaaaac tataaataaa
    17641 tctagtaaga tatatatttt ggcaagaggg cttactgaat atgtagcaaa agagtttagt
    17701 cttaaattgc aattacttga ttttaatgtt gtttttataa atgaccccaa cataatgaaa
    17761 ataaaatcta aatcaataaa aaaagacgaa cttataatca tattttcatt gaatggaaat
    17821 accatagagc ttgtagaatc agcacagaat gccaatttag ttggagctaa ggttataact
    17881 tgctgttgta gtgaaagtgc aaaacttata aagtattcgg atttatatat tgtaggatac
    17941 aaacatgaac atatatcaat ttctgaatat gaagtatctt ccagattgcc cttgtatatt
    18001 atatctagga taattataga ttatatggtc agatataata attctgaaaa ttaaattgaa
    18061 aagtctatga aaatcgattt tctatttgtg aaaagatttt catagacttt tttattataa
    18121 tgaaaacata cacagcacaa gcaaggggga agacaatgat atatacatta actacgaatc
    18181 ctgcaataga tatgaatata tctactaatg ggataaagag aaaattagta aatcgtacat
    18241 caaatgctgt atattctcct aatggaaagg gattaaatgt aacttttgta ctaggtcatt
    18301 atggtataga atctaagata ttaggatttt ttggaggatt ttcaggaaaa tatatagttg
    18361 aagaaagcga aaagagagga tttgacgtat tacctacttg ggtagaagat acaactcgta
    18421 taaatatatt tttaaatgat ggaagtgatg aatttaaatt tgtaaactct gggtcatatg
    18481 taaacgaaaa acaaaaacta gatatgctag ataagataca gtcattagca gatataagtt
    18541 acttatcaat aagtggaagt ctacctcctg gtattgatga taattactat gaggatatat
    18601 ttaaaatttg taaaaataaa agtataaaga caatactcga tataagttca ccaaaattaa
    18661 aggagttact acagtataat ccatatttga taaaaccaaa tgatgaagaa atcaaagata
    18721 tatttggaat tattgtaaga gatgaagaag atattaagga tgtacttaaa ttactgcatt
    18781 taaaaggtgc tcaaaatata ttgctaactt taggagaaaa aggttcttat ttttataatg
    18841 gaaaatcaat ttattatgct agtgctcaac cagttacagt agtcagctct gcatgtgctg
    18901 gtgattctgc attagctgct tttttaagta tatggttgga aaatccagaa catattgaag
    18961 aagcattaaa aaggtcagca gcaacaggag caagtgtggc tgaaagtaat ggtataggta
    19021 atttggagaa tgtggaagat tatataaaaa atataagagt cagaaaggtg gtataaagat
    19081 gggaaagaaa atgattctag gggtaacagg ttgtccaact ggaatagctc atacttttat
    19141 ggcagaagaa gcattaaaaa attcagcaag ggaattaggg tgtgacataa aagttgagac
    19201 gaatggagct ataggtgtag aaaataagtt aacagcaaaa gatatcgaaa tggcagatgc
    19261 aataatagtt gcttgtgata aaaatgttga tatggacaga ttcaatggaa agccggtaat
    19321 agaagtacct gtcaaagaag gtatacataa ggctaatgaa cttatacaaa ggtgtattga
    19381 tggaaaagta gctattagaa aaggaaactt atcaagcagt aaaattgcag aagaaggaaa
    19441 tttatcgttt ggtcaaaagt tgtataaaga cttaatgaat ggtgtatctc acatgttacc
    19501 tttagtagta gcaggtggag tacttactgc tatatcattt ttatggggga tttattcatt
    19561 tgacccaaat tcagaacaat ataatcaaat agcagctaca ttaaaatctg ttggtggata
    19621 ttctatgaac ttgatggttc ctgtattggc agcatttata gctcaatcta tatcgggaag
    19681 agctggtatg ttggcaggac ttgttggagg aatgatatca tttgatacag gttcggggtt
    19741 tttaggtgga atcatatcag gatttttagc aggttactta gttaaactat ttgtacattt
    19801 gttaagaaaa ttaccacgac aattagaagg tcttaaatcg atttttataa ttcctattgt
    19861 cagcgtaggt cttgtgggtg tagcaatgtt gttacttggt ggaccatgtt cagcacttaa
    19921 caatgctatg atgaactttc tatcggggtt acaaaattca agtcctataa ttttgggaat
    19981 agtaatagga tgtatgtcag catttgatat gggaggtcca gtaaataaag cagcatatgt
    20041 aactggaact atgcttctag gacagggaaa ttatttattt atggcaggtg tatctgcagc
    20101 ttgtataaca ccaccattgg taatagcaat agcatctact ttatttaaaa atagatttac
    20161 agaagaagat agagcagcag gattaatcaa ttatatacta ggtagtactc atataacaga
    20221 gggggcaata ccttttgcag caaaaaatcc attaaaagtg ttgccagtac ttatgattgc
    20281 atcatcaata tcatcaattt taacttatat aatgaaaata gaagtaccag ctccacatgg
    20341 agggtttttg gttctaggat tagtcaataa gccactttta tgggtagggt gtatatttgt
    20401 aggttcatta gtaggtgctg tactttatat gatggttaca cctaaagtag taaatgagaa
    20461 aaatactata gaaaatacaa atgcaacaaa agatggacat aaaaatgaaa gtgcagaatc
    20521 ggaaaacgta tctaataata tctctttata tagcgaagaa acagtaattt tagatgttaa
    20581 gggaaaaaat aaaacagatg taattgatga aatgattgga atacttgata aatctggtgt
    20641 tcttttagat aaaaagaagt ttaaagaaga aatccttaaa agagaagaaa tatcatctac
    20701 aggttttggt atggggatag cgataccaca tgcaaaaaca gatgcagtaa aggtaccacg
    20761 tgtagcagta ggtgtatcaa aagaagggtt tgactttgaa tcagaagatg gaagcttggc
    20821 acatattata tttatgatat cagctacaga taatggtgat aatttacact taaaaacatt
    20881 atctcaacta tcttcaaaat taatgaatga agaattttta aatgagttat taaatagcaa
    20941 aacaagtaga gaaatagtat caaaattaaa taacgaagaa atcaaatcag tataaacata
    21001 taggaggaga aattatggca ttagtgacaa ctaaacaaat attattagat gcacaagaag
    21061 ggcattatgc agtaggtgca tttaatgttg aaaatatgga gatggttatg gcagtaatag
    21121 aggctgctga agaacttaaa tcaccagtaa tattgcaaac aacaccttca acaataaagt
    21181 atgcagggct agattattat ttagcgaatg taaaggtagc atcagagaag gcaagtgttc
    21241 cagtggcaat gcacttagac catggttcaa gctttggact agctatgcag gcacttagag
    21301 ttggatatac ttctattatg attgatggtt ctcatgaaag ttacgaagat aatatttcaa
    21361 tatctaaatc tgttgtagat gcatgtatgc cttcagatat acctgttgag gctgaattag
    21421 gcaaagttgg aggtaaggaa gacgatttag atggtggtga tggtggagca tatacagacc
    21481 cattagaagc aaaagagttt gtagagagaa caggggtatc atctttggca gtagcaatag
    21541 gtacggcaca tggactatat aagggagagc caaagcttga tttagataga ttatctgaaa
    21601 ttagagaggt tgtatcagtt ccattagtat tacatggtgg gtctggaata ccagatgagg
    21661 ttattaagga atctataaaa agaggtattt gcaaagttaa ttatgcaact gaattaagga
    21721 ttgcttatag caatggggtt aaggatgtgc ttaattctga cccagaggta attgacccta
    21781 agaaatatgg taaaaaaggt ttagagtcag ttaaagattt tgttaagagt aggatggaag
    21841 tttgtggatg tgtaggaaaa gcaataggga cagtgaagtg ttaataataa agggaaagaa
    21901 taagtaagat taaattaagg ggtgtctcaa aatgaacttt ttagttcatg aggcactctt
    21961 ttttgtgtga aatcaaatat attttcatca ttctgacaat aaaaaagaag attatatcta
    22021 ttttgagaca gcatcttatt ttattttttg attaggattg tagttgagaa atttgaaata
    22081 aggaagatga tataattaaa taaaaaattt ctttctaagt ttaaattaat attaaaaagt
    22141 tataatatgg taagagttat ccaggtagat gacttctatg atggatatat gataaaataa
    22201 ttttgtatgg tatatataat aatcataaaa taagagtatt gcattagttt tttagctaaa
    22261 atatgtaaat gggaaaaata atttaataat tgtgtagtaa aaacttggat ttaacataag
    22321 acactaacta ttaaggcgta cgactaaaat atatatttaa attattttat gaacactgga
    22381 gggaaagtca tatatgatta caggagaatt aaggagtaaa gtagacaaga tttgggagac
    22441 attttggact ggtggattaa ctaatccatt agaagttata gaacaattta catatctttt
    22501 atttattaaa ggattggatg aagtagaaac aaaaaatgaa gcagaagcaa ctcttttggg
    22561 tgtttctttt gaaaagatat ttgatgatga acatcaacat ttaagatgga gtaagttttc
    22621 taatgaagga aactcagaaa aaatgtatga aatagtacaa aatgaagtgt ttcctttcat
    22681 aaaaaaatta catggaaata aagaatcagc ttatgcaaag tacatgggag atgcaatatt
    22741 taaaattcct acgcctctta tgttatctaa aatagttgat ggtattagta atttagaatt
    22801 aagtaaggat agagatacaa agggagattt gtatgagtat ttattatcca aagtggctac
    22861 agcaggtact aatggtcaat ttaggactcc aagacacata atagatatga tagtaagact
    22921 tattaaacca acaccagaag atattatagt agacccggca gcaggttcgg caggtttttt
    22981 agtatcatca caacagtact taagggataa tcattcaagt cttttcttag tgcaagggct
    23041 gaaggagcat tttaataatg gtatgttcta tggatttgat atggatagaa ctatgcttag
    23101 aataggtgct atgaatatga tgttgcatgg cgttgacaat cctaatatag agtataagga
    23161 ttctttatcg gaggttaata cagataaaga taagtttaca ttggtgctag caaatcctcc
    23221 ttttaaggga agtttagatt atgaagcagt gtcagcagat ttattaaaag tgactaagac
    23281 aaagaaaaca gagttattat tcttggcact ttttttaaga gttttaaaaa ttggtggaag
    23341 atgcgcatgt atagtgccag atggagtatt gtttggttca acaggtggac ataaatcgat
    23401 aagaaaagaa atagttgata atcataaatt ggaagctata atatcaatgc caagtggtgt
    23461 atttaagcca tatgcagggg tatctacagc tataatgata tttactaaaa cagggacagg
    23521 tggaactgat aaggtatggt tctatgatat gaaagctgat ggttattctt tggatgataa
    23581 gagaaatgag ataaaggata gtgatataga ggatattatt aagagatttg ataatcttga
    23641 tggggaagta gatagaaaaa gaacagagca aagctttttt gttgatgtgg aagagataag
    23701 agagaatggg tatgatttat ctattaataa gtataaagag gtagtgtatg aggaagtagt
    23761 ttatgatgct cctagtgtta ttataggtag ggttaaggtg ttagagaaag agatagtgga
    23821 tggattagag gaactagaaa gaatgttaga ggtgtagagt atggttgatg aagcaaaaat
    23881 tttagatgtt gttagtataa gcagggaaaa tgtaaaaaag tttgatggag aacgctcata
    23941 tcttagtact ggaaacttag attttaacaa gataagcaat ctagaaatag ttacttatga
    24001 aaataaacct tctagagcaa atcaaactgt aaatatagga gaagttatat ttgcaaaaat
    24061 gaaggacaca aagaaaacac tagttattaa taaaactaat aaaaatataa tagtatcgac
    24121 tggcttttat gttttaaaac catcaaaaga aa
//
LOCUS       DACTGW010000042        23517 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.042, whole genome shotgun sequence.
ACCESSION   DACTGW010000042 DACTGW010000000
VERSION     DACTGW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.042"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            41..412
                     /locus_tag="I9573_001295"
     CDS             41..412
                     /locus_tag="I9573_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAT4795922.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            544..1818
                     /locus_tag="I9573_001296"
     CDS             544..1818
                     /locus_tag="I9573_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAT4795923.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKSKNNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            1929..2873
                     /locus_tag="I9573_001297"
     CDS             1929..2873
                     /locus_tag="I9573_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795924.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            3089..4426
                     /gene="rsxC"
                     /locus_tag="I9573_001298"
     CDS             3089..4426
                     /gene="rsxC"
                     /locus_tag="I9573_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAT4795925.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            4449..5426
                     /locus_tag="I9573_001299"
     CDS             4449..5426
                     /locus_tag="I9573_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HAT4795926.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            5426..5995
                     /locus_tag="I9573_001300"
     CDS             5426..5995
                     /locus_tag="I9573_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HAT4795927.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQENNEL
                     RKAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            6005..6601
                     /locus_tag="I9573_001301"
     CDS             6005..6601
                     /locus_tag="I9573_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAT4795928.1"
                     /translation="MMNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLS
                     TAAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYI
                     PLIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGM
                     TLFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            6616..7191
                     /gene="rsxA"
                     /locus_tag="I9573_001302"
     CDS             6616..7191
                     /gene="rsxA"
                     /locus_tag="I9573_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAT4795929.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            7207..8178
                     /locus_tag="I9573_001303"
     CDS             7207..8178
                     /locus_tag="I9573_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HAT4795930.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            8398..8982
                     /locus_tag="I9573_001304"
     CDS             8398..8982
                     /locus_tag="I9573_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAT4795931.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            9027..9686
                     /locus_tag="I9573_001305"
     CDS             9027..9686
                     /locus_tag="I9573_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAT4795932.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            9756..10817
                     /locus_tag="I9573_001306"
     CDS             9756..10817
                     /locus_tag="I9573_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAT4795933.1"
                     /translation="MAKEKKKEKKGFFSFNKMTKDMGIDLGTANTLVYIKGQGIVVRE
                     PSVVAIRDDSKEVLAVGEEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQK
                     AADKKGVVSPRIAICVPFGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVE
                     EPEGNMVVDIGGGTSEIAVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERT
                     AENVKINIGSTFKDDEEINMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIK
                     STLERTPPELASDIMENGIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTG
                     KSVEDQEIFEKVLMMNTKN"
     gene            10827..11720
                     /gene="mreC"
                     /locus_tag="I9573_001307"
     CDS             10827..11720
                     /gene="mreC"
                     /locus_tag="I9573_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAT4795934.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKDMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            11735..12223
                     /gene="mreD"
                     /locus_tag="I9573_001308"
     CDS             11735..12223
                     /gene="mreD"
                     /locus_tag="I9573_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAT4795935.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            12235..15213
                     /locus_tag="I9573_001309"
     CDS             12235..15213
                     /locus_tag="I9573_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAT4795936.1"
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIVNGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            15311..15994
                     /locus_tag="I9573_001310"
     CDS             15311..15994
                     /locus_tag="I9573_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAT4795937.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYENESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            16018..16815
                     /gene="minD"
                     /locus_tag="I9573_001311"
     CDS             16018..16815
                     /gene="minD"
                     /locus_tag="I9573_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAT4795938.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            16833..17117
                     /gene="minE"
                     /locus_tag="I9573_001312"
     CDS             16833..17117
                     /gene="minE"
                     /locus_tag="I9573_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAT4795939.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            17202..18332
                     /gene="rodA"
                     /locus_tag="I9573_001313"
     CDS             17202..18332
                     /gene="rodA"
                     /locus_tag="I9573_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HAT4795940.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            18373..18786
                     /locus_tag="I9573_001314"
     CDS             18373..18786
                     /locus_tag="I9573_001314"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAT4795941.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            19271..19600
                     /locus_tag="I9573_001315"
     CDS             19271..19600
                     /locus_tag="I9573_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAT4795942.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            19604..20395
                     /locus_tag="I9573_001316"
     CDS             19604..20395
                     /locus_tag="I9573_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795943.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTNIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            20959..22041
                     /locus_tag="I9573_001317"
     CDS             20959..22041
                     /locus_tag="I9573_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAT4795944.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGESVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQEEAILDEVYGNNLRPKIERYEGVPCSLINSSINTIKFTSKNSNLDPS
                     RHTVSVIGFMNTPYGMVALAPDGVPVEYPFEYDKPFSLDFTGKNLSAAELSKKCKNNN
                     VIIKIEIGEIEPNSGRGQVRYARYVN"
     gene            complement(22253..23446)
                     /locus_tag="I9573_001318"
     CDS             complement(22253..23446)
                     /locus_tag="I9573_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HAT4795945.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
BASE COUNT         8995 a         2761 c         4535 g         7226 t
ORIGIN      
        1 tataatataa aaaaaataaa aattggagag tgatttcaaa atgaagttta atttttgcca
       61 taacaatttc aatgttacta acttagaaaa aagtttagat ttttataaaa aagctttagg
      121 gcttaaagag gtaaagcgta aggaagcaga agatggaagt tttatattag tttatttagg
      181 agatggtatt actagtcata cattagaatt aacttggctt agagattggg acagaccata
      241 taatttagga gacaatgaat ttcatctggc tttagaagtt gaggagtttg atgaggcaaa
      301 aaaacttcat aaagatttag attgcatatg ttttgaaaat gaaagtatgg gaatttattt
      361 tatagcggac ccagataatt attggattga aatacttcct aaaaatcatt aattttaagg
      421 atatgtttat aatttcttta caattaataa gagaataagt tatcattaaa ttgtagggac
      481 taaaaatgga taataatgtt atgaatttat gactataaaa atttgttggg gggagtgata
      541 gtagtgaaga aagctatagc agctttagga attggggcag tagcagtatc tgttagctcc
      601 ataaatgcaa gtgctcttga aaagggaaca gtaacagcaa gtgccttgaa tataagaagt
      661 ggaccaagtt ctgattgtga taaggtagca aaattataca agggaaaaac tgtagagatt
      721 ttagaaaagt ctaatggatg gtataaagta agggtatcga gttctgttgt tggatgggga
      781 agcgcaaaat acatatcaac aagtggttca tctgaaggta catcaaatca aaataatcca
      841 acttcaagtg ggacaactat tagtggaaat ggtaaagtaa atgtgagttc tagactgaat
      901 gtaagaagtg gtgcaggtac taactattca ttagttggta aagcaaataa tggagatgta
      961 gtaaagttac tagaacaaag caatggatgg tataaaataa aactatcaaa tggagttact
     1021 ggatgggcta gtagccaata tatctctaaa acatcagagg atgttggaac gaataattct
     1081 agtaattcaa atagtactaa taattctgat aaaaaaccat ctagcgaaga atcaattgaa
     1141 ggcaagaatg gtaaggtaac atctgctgtg agtttaaatg taagaagtgg tcctgggaca
     1201 agctattcta taataggaaa attaaatggt ggagatgttg ttgaattaaa atccaaaaat
     1261 aatggttggt ataaggtaaa gttatcgagt ggaacaattg gatgggtgag tgccagttat
     1321 atttctgaga ctaatgaagg tacaaaagaa aaaccaaatt catcatctaa ccaaaattct
     1381 caatctaata gtaactcaaa gccatcattt actggaaatt cagataaatc tacagcaaaa
     1441 ggttcaacaa tcgtagattt tgcatatact cttattggaa taccatatca atggggagct
     1501 tcaggacctg ataaatttga ctgttctgga tttacacagt atgtttttaa acattctgta
     1561 ggcgtttcta ttcctagagt ttctagagaa caggctaatt ttggaagtgc aatttctatg
     1621 gggaattatg caccaggaga tttagtttat tttgacactg atggtgatgg aacaactaat
     1681 catgtaggta tatatgtagg aaatagtaaa tttatccatt gtagtggtac tcagactaat
     1741 cctaataagg ttaaagtgga taatttaact agttcatatt ggtctaaagt attacttggt
     1801 gcaagaagat ttgtttagta agtacaattt cttattaaaa aattagaaga aataattatt
     1861 tatattcata aattgtgact cttaaaaata tactttatta ataatataat tttgggaggg
     1921 acaaactaat gaatagaaga aaaacgatat acagaggatt taataataat agacataaga
     1981 ttgatttacg taaatatgtt ataacgattg cttgcttgtg tctaattggc tattattctt
     2041 atacaaaaat aaaagatagc aagatattgg agtatgtatc ggctaaaata ccatttctaa
     2101 ataattcatc agatataact tataaagata tatctgatga attaaattca ataaaaaaag
     2161 ggaaaaaatc taaatcacaa accaattcag atgataaaca agaaactaat ccagaaaaag
     2221 cggtaaataa cactaaagag ccagaagaag tcaaattagc tactatagaa ggctgggata
     2281 tgtacactat tcaagttgct gcaatagata ataatgatga tttgaaaaaa atacagacat
     2341 cattagttaa taatgacata cctttttcag ttatggagaa ggatggagta aaaaaaatac
     2401 agacctattc ctcttttgat gaaaatgatg ttagaaagca gataagcagt gtaagaaagg
     2461 tgtttccaga tgcattttta tcacacttag atgcacctat gttgtcttta gagtatacaa
     2521 gtaactacgc ttatatagag agtatatcta aagaattaaa taaattaata acaaacttta
     2581 aagaagaatc aagtttttgg tcgaatgctg aaaataatgt agatatggaa aaatataata
     2641 ctattttgac aaatagaaag gcaatatcac aaaatatatc aaaagaagct gaaaagattg
     2701 actattctga aatgcgttta tttaaagata atttaataga gtatgtaaag aatgtgaatg
     2761 aaaaaattga tacagcttca aaagcagcaa atgaagaaaa atatagtgta agtaaaagtc
     2821 tgcttttatc atccatgcag gagtactcta tgtttataaa ttctataaaa taaaatacag
     2881 aaaatttgga ctttagaaag ctatatgaaa aggattgttt aattaagcaa tccttttcct
     2941 cagtttgact taatatagag gtattgattt ataaaataaa ttataaaaat acggatattt
     3001 ttttaacaat tgttaagatt ttttaatata tatagtatac ttatagaagg cagaaaaaat
     3061 gtttttttat agataagggg gtaaaacaat gaaactctta acttttaagg gagggataca
     3121 tcctccatat agaaaagagt actctaacac aaaggcactt gaaaaagctc aagctccaaa
     3181 gatagtttac attcctcttc agcaacatat aggggcacca gctaaaccta ttgttgaagt
     3241 tggtgatgaa gtaaagtttg ggcaaaaaat aggtgaacaa caaggttttg tttcatgtaa
     3301 cgttcattct tcagtatcag gtaaagttat tgctattgaa caacatgaag taccaggagg
     3361 ttcagctcaa tgtgtagtta tcgaaaatga ctttaaggaa gaattacatg agagtgttca
     3421 accaaaaggt cagttagagg atttgagcaa agaagatata gtaggtatca taaaagaggc
     3481 aggaatagta ggtatgggtg gagctacatt ccctaaccat gtaaaagtat ctcctccacc
     3541 agatagcaag gcagaagtag ttatattaaa tggtgcagaa tgtgaaccat acttaactgc
     3601 agaccacaga ttaatggttg aaaatccaga agatgttgtg tttggtttaa gagcattaat
     3661 gaaggtatta gatgtcaaaa aaggatttat aggaattgaa acgaataaac cagatgctat
     3721 agaagcaatt caaaatgtag ctaaagacta tagtgaaatc gaagttgttg gccttcaagt
     3781 gaaatatcca caaggagcag agaaacaact tatttatgcg tgtacaggca aagaagttcc
     3841 atctggtgga ttaccaatag cagcaggtgc agttgttgac aatgttgcca cagcagcaca
     3901 aatagctaag tctattaaaa caggtatgcc tttggttgaa agaatcacta caataacagg
     3961 tagttgcatc aaagagccaa agaacctaat aacaaaagtt ggaacattag tttcagaaat
     4021 aatagaacaa tgtggtggat ttaaagaaga taagaagatt ggtaaggtta taatgggtgg
     4081 acctatgatg ggaatagctc aatatactac tgaaatagcg actaacaaag gttcttcagg
     4141 aattttatgc ttggatgagg aagaatcacg tactccagat attcaaaact gtttaagatg
     4201 tggaagatgt acagatgtat gtccatcatt cttacaacca cttttcataa gtgcatattc
     4261 tttaaaagat gactatgaca cagctgaata tcatagagca atggattgta tagaatgtgg
     4321 gtcttgttca tttatttgtc cagcaagaag accattactt caatctataa gatcagcaaa
     4381 gagagaaata ggagcaaaga gaagaaagca agctgctcag aaataaagaa agttaagggg
     4441 gaaaaacgat ggaaaataag ttgatagtat catcttctcc tcatgtgaga agtaatgaag
     4501 atacttcata tataatgaaa caagttatta tagcactcct tccagcagca gtagcaggag
     4561 tatacttctt tagacttaat gctttgagtg ctatgttttt ttgtatactt ggtacagtag
     4621 gtactgaatt tttatatcaa aaatttacga agcaaaaaag tactatagga gatttttcag
     4681 ctgttgtaac aggattatta ttagcattta acgtaccagc atcacttcct tggtggatgt
     4741 gtctagtagg aggaatattt gcaatattag tagttaaaat ggtatttggt ggaattggat
     4801 gtaactttgt caatccagca cttgctgcaa gagcattttt attagcatca ttcccagtag
     4861 caatgactgc ttggactcaa ccaggtgtta actggatagg taaaaattta gatgcagtta
     4921 ctacagcaac tccattaagc tttttgaaaa atggagcagc aggactggca gacctttcta
     4981 gcaatggaat tagtcttgct gacatgatga tagggaatat tggtggatgt ataggtgaga
     5041 catcagcaat attattatta ttaggtggag tatacctaat gtataaaggc ataataaatt
     5101 atgttatacc agtattttac atagcaactg tatttatatt aacattcctt ttaggtggat
     5161 ttaatataac tttcgcaata tatcaactgt ttgctggagg acttatgtta ggtggattct
     5221 tcatgcttac ggattataca acttcgccta tgactaaaaa aggtcaaata atatacgctg
     5281 tattagctgg tcttataaca actgttataa gaatgtatgg tggatatcca gaaggtgtat
     5341 cttactcaat actattagtg aactgtcttg ctccacttat agataagttt gttagaaata
     5401 gagtatttgg ggaggtggct aaataatgaa cagtatggtg agattaggtg gtactttact
     5461 tgctataagt gcaatagctg cattagctct tggagctact aatcaggtta cagctccagt
     5521 aatagaacaa agaaatatac aagaaaataa tgaacttaga aaagcagtcc ttccagaagc
     5581 aaaagaattt aaagaaatgg ataaaagtac atataaagac cttggagatg gtcttatagc
     5641 tgaagtgtat gaaggtttag atggttctga agtggttgga tatactttaa aagctaaacc
     5701 aagtggatat ggtggagaaa ttgaagttat ggtaggtata tcatctgaag gtcaagttac
     5761 aggtgttgac attggtaata tgtcagaaac agcaggtctt ggagcaaaag ctaaggatga
     5821 tgcatttaag ggtcaataca aaggaaaaac tgctgagcct cttgaagtag caaaaggaag
     5881 tacaacagca gataatcaaa tattagcaat atcaggtgca actattacat ctacagcagt
     5941 tactacagga gtaaatgctg ctatagatgt tttcaatagt gctcttaata aataaggagg
     6001 gttaattatg aatttagcta aagtatttaa aaatgggcta atagatgaga acccaacatt
     6061 tgttcaagtt ataggtatgt gtccaacact agccgttaca acttcagcaa taaatggaat
     6121 tggtatgggt ctttcaacag cagcagtttt aatatgtgca aatttagtta tatctttaat
     6181 aagaaaaatt acaccagata agataagaat acctatattt atagtagtta tagctacatt
     6241 tgttacaata gtaggtatgc ttttaaaagc atatgttcct gctcttgata aggctcttgg
     6301 tatatatata ccattaatag ttgttaactg tttaatatta gctcgtgcag aaagttttgc
     6361 tttcaaaaca ggagctatgc cttcaatagt tgatggtgta ggtcaaggtc ttggatttac
     6421 tgttgcactt acaataatag gtgcagtaag agaattactt ggaaatggta gtttatttgg
     6481 aatgacatta tttggagcat ctttccaacc agtattaata tttatattac caccaggtgc
     6541 attcttaaca ttaggattct tatttgcagg atttaataaa ttaagaagta aaaaagcata
     6601 gtggaggtgt aataaatgaa tttaatactt ttatttttaa gtatcgttct tgttaataac
     6661 gttataacat ctcaattctt gggtatatgt cctttcttgg gagtttctaa gaaggttgat
     6721 acagcagtag ggatgggagt tgcggttacg ttcgttttaa ctctagcttc aattataact
     6781 tattttatac agattttatt agtaaaaact ggaactggat tcttacaaac aatagctttt
     6841 atattggtta tagcttctat agttcagttc gttgaaatgg taattcaaaa gatgagtcca
     6901 tctttatatc aagcattagg agtttattta cctcttataa ctacaaactg tgcagttctt
     6961 ggtatagctc tagttaatgt tcaaaaaagt tataatttag tagaaacaat aataaatgga
     7021 tttggagccg gagctggatt tactttagca atagtaatat tcgcaggtat aagagaaaga
     7081 ctagagttag ctgatatacc agaagcattt aaaggtttcc ctataacgct tatatcagca
     7141 ggtttaatgt caatagcttt cttaggattt acaggactta taaagctata aaaaagaggg
     7201 gtgaaaatgg tgatacttac agctgtatta gttttaggaa ttatgggctt aatcttcggg
     7261 atagtactag atttcgcatc taaaaagttt gctgtagaag tagacgagag agtggaagct
     7321 atactaggag tacttccagg agccaactgt ggaggttgtg gatttccagg ttgtggaggt
     7381 cttgcaaatg cgatagttga aggcaatgca ccagttaatg gttgccctgt aggtggagca
     7441 gatgttggtg caaaagttgg agaaataatg ggaataagtg ctgaagcagg tgagaaacaa
     7501 gtagcaaaag ttatttgtaa aggaacttgt tcaagtgcaa aagataaata tgagtatgaa
     7561 ggaatatcag attgtagagc agcaaatgtt ttaaactcag gtgctaagat gtgtaaattt
     7621 ggatgtttag gtcttggaac atgtaaggat gcttgtaaat ttgatgctat ttctatagtt
     7681 gatggaatag cagtaataga tgaagaaaag tgtgtaaatt gtggaaaatg taaagaagtt
     7741 tgtcctaagg gtataataat cacaaaacca gaaagtcaag aagtagttgt agagtgtaat
     7801 agcaaagagt ttggtaaagc tgttaaggaa aaatgtactg ctggatgtat tggctgtgga
     7861 atgtgtgtaa aagcttgtaa atttgatgca ataatatttg aagacaaaat agctaagata
     7921 gaccctaata agtgtgtagg gtgtatgcaa tgtgtagcaa aatgccctac aaaagtaata
     7981 tcaggtgaca taactaagaa aaagaaagtt actattgacc aagaactttg tgttggatgt
     8041 acagtgtgca aaaaacaatg taagtttgat gcaatagagg gagaattaaa agaaaaacat
     8101 aaggtagatg cagataaatg tgtaggatgc catttatgta tggaaaaatg tccaaagaaa
     8161 gctattaaaa ttttgtaata tggacaaaat aaggatatgg aataatttcc atatctctaa
     8221 tgagtacata aaacagaggt atggaataat ttccatacct ctattttatg tatatttatt
     8281 ctaatggtaa taaatataac aatagtgtaa caaaaaaggt tcgacatttc tgttgaattt
     8341 tttacaatat agtgatagac taaataggta caaattacgt acaaatgagg tgaataaatg
     8401 aatataatct tggcatcagc ctctccaaga aggaaagaaa ttctagagaa tacgaatgta
     8461 agatttgata taattaaaaa tgaaattgat gagattatat tggaaggtga aacaccaaaa
     8521 catttagtta tgagattagc ttttgaaaaa agtatgtctg tagcatctga acataatgaa
     8581 gatgttgtta ttggagctga tacagttgtt gttttagaca atgctatttt aggaaaacca
     8641 aaagatgagt cttgtgcaag agatatgtta aaaagactat ctggaagaga gcatcaagtc
     8701 ataacaggta ttagcttaat aaacttgtgc gaagataaaa aggtaattga ctatgtaatc
     8761 agcaatgtga aatttaaaac gttatcagag caggatattg aggattatct gaaaactaat
     8821 gagtcatttg acaaagcagg agcctatggt atacagggtt atggagcttt gttagttgaa
     8881 gaaattagag gagattattt taatatagtt ggtcttccaa tttcaaggtt aggcgaccta
     8941 ttaaaaaaat attttagtat aaatcttttt tatggagtgt gatttatctg aaaaagtcat
     9001 ttaattcaac cattaaggta aaagaaatgg caccagagga aagacccaga gaaaagatgc
     9061 ttgccaaagg cgtaaaaagc ttatcaaatg ctgagttgtt ggctatactt ttaagaactg
     9121 gcaacaagaa taaaaatgct attgaattag ccaactatat aataaataga gatattcagg
     9181 gtattagaca tttagaagat atgacaatag aggagctgtg taatatagat ggaattgggt
     9241 tatcaaaatc aacccaaatt aaagcagctt tagaactagg ctctagagta gctagtttta
     9301 aacctataaa gtataaaata aggaatcctt gggatataca gaggtattat atggatagcc
     9361 taaggtattt aaaaaaagag gtttttaaag cagttctttt aaacacaaaa aatgagataa
     9421 tatctgatgt agatgtatct atagggactt taagctcgtc attagtccat ccaagagagg
     9481 tttttaaaga agctataaga agaagtgcaa gtaagataat agtgatgcat aatcacccct
     9541 caggaagtgt agaaccatca agagaagata aaaatatcac atcaagactt attaaatgtg
     9601 gagaaataat tggaatagaa ataatagacc atataattat tggagatgga ttatacttta
     9661 gctttaaaga aaatatgata atttgacttt atatgtgata aaataatagg agaatgtttt
     9721 aaacatgatt cttaatagta ggaaggagta aaatcatggc taaggaaaaa aagaaagaaa
     9781 aaaaaggttt ttttagtttt aataagatga caaaggatat gggaatagat ttagggactg
     9841 caaatacatt agtttacata aaaggacaag gtattgttgt tagagaacca tcagttgtcg
     9901 caataagaga tgatagtaaa gaggtattag cagtaggtga agaagccaaa aaaatgatag
     9961 gtagaacacc aggaaatata gtagctataa gacctatgaa agatggagta atagctgatt
    10021 ttgacataac tcaatcaatg attagctatt ttatacaaaa agcggctgat aaaaaaggtg
    10081 ttgtaagtcc aagaatagct atatgtgttc cttttggagt tacagaagta gaaaagagag
    10141 ctatagaaga ggctgcaaga caagctggag ctaaagatgc attccttata gaagagccaa
    10201 tggcagcggc tataggggct ggattaaaag ttgaagaacc agaaggtaat atggtagttg
    10261 atattggtgg aggtacttca gaaatagctg taatatcctt aggaggaatc gttactgcta
    10321 aatctataag aataggtgga gacgaatttg acgagtctat agtggcttat gtaaaaaaag
    10381 aatataatct tatgataggt gaaagaactg ctgaaaatgt aaaaattaac ataggttcta
    10441 catttaaaga tgatgaagaa ataaatatgc aaataagagg tagagattta atctcaggat
    10501 tacctaaaac aatagaaata tgctctacag aagttagaga agcattaaaa gaacctgtaa
    10561 gttcaatagt agatgcaata aaatctacac tagaaagaac tccaccagaa ttagcctcag
    10621 atataatgga aaacggaata atgctaacag gaggaggcgc tttgcttaga ggtcttgata
    10681 agcttattac acaggaaact ggtatgtctg tacaaatagc agaaacacct cttgattgtg
    10741 ttgcacttgg aacaggtaaa tctgttgaag accaagaaat ctttgaaaaa gtattaatga
    10801 tgaatactaa gaattagaag ttggtgatgg ccttgagatt tgacaagaac aaaaatgaga
    10861 aaaaaaggat taatgttaaa gtgatagcaa cgggcgtagt tgctatcact ttaattggaa
    10921 ttgtgggtat atcaattggt aaattttcta gtggtagtcc agtaaattta ggagtagctt
    10981 cagatgctat tacaagtgta ggtaagggaa taaatgatgg attttctttt atcaaaaatg
    11041 gttttaaaga tgtagcaaat tttaaagata attctaaaaa ggtaaaaaaa ttggaagaag
    11101 aaaatgaaaa acttaaaaaa gatatgatag ctttaaatgc caaactagat aaaacagaat
    11161 cgctagaaga gttaaaaaag acactgaact ttgtacaaga agagtataag gcgacaagta
    11221 tttcagcaag tgttgttggt aaaaatgatg gaaactggta tgagagtttt gtcataggtg
    11281 caggaaaaaa ttcaggtgta aaaaaagaaa gtatagttat gaatggtagt gggttagttg
    11341 gcatagttta tgaagtttcc aacaattata gtaaggcaat ttcactattg gattctaagg
    11401 catctgtaag ctttaaacta gcaaaggatg ccaatgctaa aggtacaatt actcaaaata
    11461 ctactttaga taataaagat agttataata gtaaaggata tttgcaagga tatatgtttg
    11521 actcatcata taatgtaatt caaggagata taataacaac atcaggtctt ggattcttcc
    11581 cagatggtat accaattggc gaagttgaaa aggtggttga tgataaagat aagtcattaa
    11641 aatatgtagt tgtaaaacct tatgttgatt ttaagaatat aaatgacgtt gtagtcatag
    11701 aaccaagaaa tataggataa aggggataaa aacaatgaaa aaagttttac tttgtctgtt
    11761 ggggattgtg cttatgacta tagaaaatag tataacaaat tatatagata tttttggcat
    11821 aagctttaat ttagttctta tatatgtaac cataatctct ctttatttag atgaattaga
    11881 aggtgggatt atagcagcaa taatagggct tattaaagac ataaccgttg gtggaatttt
    11941 tggtgtaaat gcattaattt tgtttattat tgcatatgca attggataca tgagagatca
    12001 gctatataaa ggtagttata ttattacttt cgttttaatt tttataggca gtttaattga
    12061 ttcaattgtc aatataggta catctagtat aatttatcaa agctacaaca tatcaacact
    12121 ttttgtaaaa ggtctactag tagcaccaat agtgaatagt ttaattggtt tagtaatata
    12181 tcgcttatct aaaagagcag ttttaaaact taaagaagat tagttggtga tttcatggaa
    12241 gaaaataaac aagttgatag actaaaaata attaagatag tcatgagcgt gatattttta
    12301 gctattttag taaaaataat ctatatgact acatttaaat atgaatatta caatgagtta
    12361 gctgagaata aaacatataa aaagttggca atagaagctc caagaggaga aataaaagat
    12421 agatatggaa gactgttggc aggaaataaa aacttattta ctgttcaagt ttcaggtaac
    12481 gatattaata agaaagatgc aaataagcat tcaagagcaa atgaaatatc attaaaactt
    12541 ataaatttgc tggagagaaa tggtgaagaa tatgtagatg agtttcctat atatgtagaa
    12601 aatggaaagt actattatac atatgacaga gatattaggg aatataaatc agaaaatggt
    12661 attccaaatg attataatgc taaggaaagt ttttattact tagtagacaa acttatatca
    12721 gctggaatat tatctcaaga agataaaaga ttggatgcga caagattaca ggcaaaacta
    12781 aatgaaaatg gatattatcc acctatttta gttagtaaat ggatgttcac agcagaaaga
    12841 gataagagag attggttggc tagttataag ataaaagaaa ctaaactgag tgccaaagaa
    12901 gcgtttgaaa aagtaagaaa tagtgatgca ttagaaattg ataaaagttt gagtgatgaa
    12961 gatgcaagaa aaataatggt cgtaagggat ttgataaaat caaaaggata ttctcaatat
    13021 aatccagtaa ctattgctaa ggatgtagga gaaactacga ttgctcaaat tgaagaaagt
    13081 gctatggacc ttgttggtgt ttcaatagca gtagaaccag taagatatta tcctaatggg
    13141 tcattagctt ctcatatgtt aggatatgta ggaaaaatgc catccactca aatagaatca
    13201 taccttcaaa aaggttatga aactggagat atggtaggtc ttgcaggagt agaaaagagt
    13261 aatgaaagta gattaagagg aactgatggt tataaaatgg ttaaagttga tgcattgggt
    13321 agaatcagta aagaaattga atctaaaaaa cctaaatcag gagatactgt gtatcttaca
    13381 ctagataaag atttacaaga agtttcagat aatgcattaa aacagataat cgaagttgct
    13441 tctaaaggag gtacttttaa aagtaaattt ggggacaaac caattagtgc atatgcagga
    13501 aaagcacagt cagcagctct tatagctatt gatgttaaaa atggagaagt attagcttca
    13561 tcaagttatc ctaattatga cccaaataaa tttgcaaaag gtatttctac agaggattat
    13621 aaagcacttc aaccaaaaaa tccaaatgac ttactagcag gaagtccact tttaaacttg
    13681 gttacacaag gggaatttca gccaggttct tcatttaaga tggtaacttc aatggcagca
    13741 ttagaaaatg gtttggatcc aaactttact ataaatgacc ctggggttat aatgttaggt
    13801 aaaaaatctt ttggtgacta tgtatggaac catggtagag gaaaccatgg tatgacaaac
    13861 ttatataaag cgatacaaga gtcttgtaat atatatatgg caacaattgg tacaggtaaa
    13921 acatggccag atggaaaaag cattggtata gatatgaatg caaataaaat acttgagtat
    13981 gcaaaattat ttgggcttga ccaaaataca gggcttcaag atgaagtaga agaaagagca
    14041 ggtaaagttc ctagcacaga agataaattg aaatcaacac aagctcttct aaagtcaaat
    14101 ttagaaagag aaatggcaaa tgattttgtt gatataacta gagaaaaaaa tcctaaagaa
    14161 tatgagaaaa gaatcaatga gattgtaagc tgggcagcag aaaagaaaac tcctggtaga
    14221 gtcgaaacaa tgaataggct aaagaaaatg aatgttaaag aagatagaat agaagaagta
    14281 gcagatttag cagtattcag ttattttaac tttgcaaaat ggagtactgc agatacattt
    14341 aacttagcta taggtcaagg tgaaaatgct tatacaccag ctcaaatatc aagatatgta
    14401 gctgctatag taaatggagg taatttagta gaattatctg tagttgatag agctgtttca
    14461 agtgattatt catcagttaa aattaatgac cagaagaaag tcgaaaaaat accttttaaa
    14521 aatccagata acttaaaaga attaacaaaa ggtatgaaat tggttgcaag acaaggtaca
    14581 gcgaagagtg cttttgctga ttttccaata gatgtagcag caaaaactgg tacagctgag
    14641 aaaagtggga aaatacctac agataacgag tatgaatatt taaaaagtca tatgtcatct
    14701 tataatgtca acctaaatga tgcgattaaa ctagctgata agatgaaagc agaaaaagaa
    14761 aaagagcttt cattagctaa ggaaaaggaa ataaaaaaga aattagaaaa taaagatttg
    14821 aaagacgaag aaagaaagaa attagaggaa gaattagaag atggagtaaa agttagatta
    14881 gaggacactg ataaggtgaa ttcttcatat ctaagaaaag ctataaaaga attaaatcca
    14941 aaaataacag atgaccaaat agatagattt aaacaagatt atggttcttt tacatggact
    15001 gttgcttttg ctccagcaga tgacccagaa atagctgttg tatgtgtaat tcctcaaggt
    15061 gattcaagtg tattctcact tttaccaact agagaagtta ttggaactta tatgggatta
    15121 gagccaacta atagtaaaaa tgacaacaag acgaatgatg ttaacaattc ttctgacgaa
    15181 aatataaatt tcgagtcaca aattaataga tagcttaatc tatgtataaa ctttagcttt
    15241 atatagaaaa aataaaaggg tttaaagcgt atttgaagaa tataaatgat atatcactct
    15301 ggaggttgtt atgtctttaa gagaaatttg tagtcaagaa ctagtagagt tcaagggtaa
    15361 taaaagaggg ataatagtaa atataaaaag agaagctcca tttgaggaaa tacaggaaaa
    15421 aataataaac aaattggaag catatgttgg cttttttaat ggggctaaaa ttagtaaaat
    15481 taatagtgat tgtttaactg acatggagat tctagaactt aaagaaggaa ttacttctag
    15541 atttgatgtg gaatttgttg aagatcaaaa aattgaagaa aatagtaatt ttccaacaaa
    15601 atatgtcaat accctaagat ctggtgaaaa tattgaattt gaaggtgatg tagttatatt
    15661 gaatgatatg aaacctggtt caaaagtcct ttcaaaatca aatacagttg ttatgggaga
    15721 tataaatgct ggtgctaagg ttgtagcagg aggtaatgtt tttgtaatgg gtaaaataga
    15781 aggttttgta catgcaggtg cagaaggcaa tgaatttgca tatgttgttg ctggaaatct
    15841 taatcctaaa atattacaaa tagcagataa tattgcagaa gctccagatg atgaagaaaa
    15901 ttatgaaaat gaatcagaaa ttagcccaga aatagccttt gtaagtaatg gaagaattgt
    15961 aatcgaaagc tatttgtcaa agttagacaa atagaaagaa aaataggggg tagtattatg
    16021 agcgaagtta tagttataac atctggtaaa ggtggggttg gaaaaactac taccgcagca
    16081 aacttaggaa ctgcgctaag cctagaaaat aaaaaaacag tagttgtgga tgcagatatt
    16141 ggacttagaa acttagatgt agtaatgggt cttgaaaatc gaatagttta tgacatagta
    16201 gatgtagttg aaggaacttg taggcttaag caagctctaa taaaagacaa aagatttgat
    16261 aatctatatt tattaccagc agcacaaact agagataaaa atgctgtctc agttgagcag
    16321 atgattgatc tgtgtgagaa actaaaagaa tctttcgagt atataataat agactgtcca
    16381 gcaggtattg agcaaggctt taaaaatgca gtagctgggg cagatagagc tattgtagtt
    16441 actaatccag aaatatcagc agtaagagat gctgatagaa taataggtct attagaagca
    16501 aatgaaataa aagagataag attagttata aatagaatta gaaatgatat ggttaagcgt
    16561 ggagacatga tggataaaca agatataata gaaatattag caatagattt gttaggtctt
    16621 gttcctgatg atgaaagcat aattatatca acaaataaag gagagccagc tatacttgat
    16681 tctaagtcac ttgctggtca agcatacaaa aatatcgcaa aaaggatact aaatgaagaa
    16741 gttcctctac ttgatcttga agttgaagat ggattctttg gtagacttaa aaaaatgttt
    16801 agcatggcta agtagcaaag gaggatacat ctgtgttaga tttatttaga gttttttcta
    16861 acgaggctaa aactagtaaa tctgttgcta aagagaggtt aaagctagtt ttagttcatg
    16921 atagagtaga ttgttcacca caacttttag agatgataaa aacagacatt ttaaaagtaa
    16981 tagctaatta tgcagaaata gaagatgatg gtcttgaaat aaaaatgtct aaatgcagag
    17041 gtgagcatga tgacaaacct gtatctgcat tagttgctaa tataccacta aagaatataa
    17101 aagatagatg tatgtaatta gtgcttaaaa gggggatatc cttattggta tatgcccctt
    17161 ttatttaatt atatgtttaa agatagaaag acggtgattc tttgaaaaaa atcaatatta
    17221 attataagtc gacaattaag ttgataaaac agttagactg gaaacttata gttacagtat
    17281 tagctatctt tatttttggg cttgttatac ttagtagtgc aacacatgct aattcaacag
    17341 gttcatataa tcaattgatt aaacaaggat tagcttttgt acttggtata ggaatgatta
    17401 tagtcatatt attttttgac tataatcttc taggcaggta ttataaagcg ctttatataa
    17461 taagtcttat tttgttagcc atagttttat tacctggaat tgggacagta aaaggtggag
    17521 ctaggtcgtg gattaactta ggaccacttg acttgcagac atctgaaata gtcaaattaa
    17581 catttgtact aagttatgca aagatacttg aatccaaaaa ggataaatta aatacgttaa
    17641 aggaagttat gcctgttgta gtttattctc ttccatttat cggattattg attgcacaac
    17701 cagacttggg aacaggaatt gtattttgtt gtatgatatt tgcaatgctg ttcacagcag
    17761 gtcttagctc aaagttgata aaaagaggaa taatcatact attagtgtca atgccactga
    17821 tgtatttaat gatggcagac catcaaaagg taagaataga agcttttcta aatccagaag
    17881 atgtaactct aaaaggaaac tatcaagtta tgcaatctct gattgcaatt ggttctgggg
    17941 gcgtaactgg taaaggactt tacaatggaa gtcaaaatca agaagatttt ttacctgtac
    18001 aagatagtga ttttatattt gctgttgtag gtgaagaact tggtgtaata ggaatggcag
    18061 tgttgataat cttatttatg atattcctat taaggctttt agcaattgca agagatgcaa
    18121 aagattttta tggaaccctc atagtggttg gtgttatggg aatgtttggt taccagataa
    18181 tccaaaatat aggtatgacg gttgcgttaa taccagtaac tggagtaaca ttaccttttg
    18241 taagttatgg aggaagttcg ttattgacat ctttagcaaa cttaggatta gtactaaatg
    18301 tgtgtatgag aagaaagaaa ataaatttct aaatttaaat acatctaaac ataaattgag
    18361 gagattagat atatgaatat agcattagta gcacatgacc aaatgaaaaa cactatggtc
    18421 ggattttgta taggttatga atcaatttta aaaaagtatg gactatatgc aactggaact
    18481 acaggaaaaa gaataatgga tgaaactgaa ttaaatataa atagattagc atctggtcct
    18541 ttaggtggag accaacaaat aggttcttta atagtaacac aagaaataga tttagttatt
    18601 tttttaagag acccattgac atctcaggct catgaaacag atatacaagc gttaataaga
    18661 ctttgtgatg tttatcatgt tccaatagct acaaatctag cttcagcaga aatatttata
    18721 aaagctcttg atagaggaga actatcatgg agagaagtta gaaaaagcaa aagtcaacgt
    18781 atttaaaaaa tattaatatt acagtctaaa gaaacagata tataagtgtt taatgcccta
    18841 gtttttctgt tgcaataaac ctagtacctg tagaaatctt atgaaagatt taaatgatga
    18901 ggagctaaat ttgagattaa ataattagga aatagattat agatagagta gtagaattta
    18961 aactattact ctattttaat aatatgaaat agaattaagt ctattattat atatatttaa
    19021 acttataggt aatactattt caaccaagta aatgttgtta cgacaacatt aataaaacaa
    19081 aaatttattt ttaaaatttg ataaatagct gtatattagt atttaatcta taaatactaa
    19141 tatacagcta tttattattt ttaagtctat tgtagaatat aaaccttgta ttatagagta
    19201 ctatgtatta ttatagtaaa tataagtact atacattata gagtagtagg tgaaaaggcg
    19261 gtggtacata atgcagttaa ataaagaagt gttaaaaggt tatatcgata tattaattgt
    19321 ttctatatta gaaaagaaag attgttatgg atatgaaatt gcaaagcaag taagagaaag
    19381 aagtgagttt gaattaaaag aaggaacaat gtatcttgca cttaaaagaa tggaatctaa
    19441 aaatttaatt aaatcttatt atagcaatga acaaagttca ggtggaagaa gaaaatatta
    19501 taatttaact aatgaaggta aagatttttt agaaataaag aaacaagaat ggagatttat
    19561 taaaaaggtt atgaatcaat ttttgggaga ggtggattaa tttatgaaac taatagatga
    19621 atatttagat aaactatata aaaaatgtga caataaaagt actatagaat taaaacagga
    19681 aatgagatgc catcttatag aatctgcaaa tgaatttaaa ttagaaggat tagatgaaga
    19741 agaagcatgt aaaaaagcaa ttgaaagatt tgatgatggt gatgaaatgc agtatgaatt
    19801 atgtaatata ataaaagaac tatctttatc attggatagg cataaatcaa ttgtaatggg
    19861 gtttaaaaaa gtattaggtt acataagtat aattgcattt ttaatttctg gatttatgtg
    19921 gtattacaat aatagtttac aacataatat gtataattta ggaaaagaac ttgatggaga
    19981 aataaagcaa ttggcagaaa gacatgatat gacaaatatt ggtgaatata agttagaact
    20041 tgaaaaaata cttgataaag ataagtatag taaagtcaaa gctcttagac tatatgtaat
    20101 agatatgaaa gatggaaaca ctaatttaag ttcttctgga ttgaatgcta atatggtata
    20161 tgagagggaa gcagattata ataacataag taattttata cagcatttag gatataatgg
    20221 aaaagatttt ttagacaaga atgggaatat agtaaatcca gatattttct tagaatattt
    20281 cttttatttt gaatcggaga tgttaattcc agtagcattt gcatttggat tattatgtat
    20341 aatagcgtat tttatattaa gatttaaaat ttctttaata aaaaataata attaaataat
    20401 tcaccaaata attttaattt gtagagtaaa taagatgttt caaaataaaa tttgaatcat
    20461 cttatttttt attgtttgtt atactctttt cttttatatt tctccatagt ggattgtatc
    20521 tatacataat cacatatttt gatataatta taccttccaa aaaatgtatt gaatatacaa
    20581 attataatct gtataatgtt gttaggggag aatgtatgaa gtgtatacaa ttttaatttt
    20641 aaatgtatta ttgacaattc tcctcactct cgtcattacc aataattaga aaattgacaa
    20701 acctgttaca gaccaacatt tttgaatata ttaattatta tcattttatt gtgatgatat
    20761 caaaaatgca ggtttgtgag ttggattgtg tacgaactat ataggaaata tagaacagat
    20821 tttccaagaa atctaaatat tctatgacca cgtgaattgc tatgcacaca tgttgataat
    20881 ccatatttta aatctaaaat gaacttaatt aatggtaggg aattgaaagt aaaatatatt
    20941 ataaaggaga atgctaatat gaaaaaaatc aggaaattag ctactaaact attgattgca
    21001 acgatggttt taacctcatg tatgagtgta acagtttatg gtatgacagc taaagatgtt
    21061 actgcaaaga caccgaaaag ctatgtagct ggaaccaaca gtatatatgg tccaaagtta
    21121 tcacaagctc aacttaattc ggtagcacaa gcaacagcag actttatgaa caagaaaatt
    21181 acaaagaaca tgaccacaga tgctaagatt agggttgctt ataatcacat taaaaacaac
    21241 acaacatatg ttaactggaa cgcagcagag ggagccaata ctgcttatac attggttact
    21301 aaaaaaggtg cttgttctgg aatgtgtagg agcttgaaag ctttatgtga tgctatggga
    21361 attgaatgtt attatgtaca tagtacaaca aatgaccatc agtggaatct tattcgtttt
    21421 gaggatggac gtttgtatca tgtagatatt gatgccaaca aatcagcagg agcagacatc
    21481 atatacaaga gtcttactca cccattacca tttgacaaga aagcctatcc agctgttggt
    21541 gaatcagtaa agacacagaa tccaacgcca actgttaaga aacctacgcc agctaagcct
    21601 tctgcgccag ttggacctgg gaatcacact atgatagcag atcaagaaga agcaatactt
    21661 gatgaagttt atggaaataa tctacgtccc aaaattgaaa ggtatgaggg agttccgtgt
    21721 agtttaatca actcatcaat aaatacaatt aagtttacaa gcaaaaattc aaatttagac
    21781 ccatcaaggc atacagtttc tgtaataggg tttatgaata caccatatgg tatggttgca
    21841 ttagctccag atggtgtacc tgttgagtat ccttttgaat atgataagcc attttcttta
    21901 gattttactg gtaaaaacct ttctgcagca gaattatcaa aaaagtgtaa aaacaataat
    21961 gtaattatta aaatagaaat tggtgaaata gaacctaatt ctggaagagg gcaagtaaga
    22021 tatgctagat atgtgaatta atataaatat acaagtatac aaatagaaaa gattataaat
    22081 aatgtaccct cttggtgagg gtgcatttat cattatatta gtcattttgg gaaataaaaa
    22141 aggagttgaa cttaaaatta gattagaaaa attaattttg agacaactcc attttaattt
    22201 atttttaaat ataaaatcat ttattttaaa taatattatg tgtgattaat ggctatttaa
    22261 ctaattttgc aaattctgca ccaaatgcca tacactcttt taatgtctct tcactaggga
    22321 agaatttttt cttaagagat tctacaggca ttttgaatga catagattta agtaaagtct
    22381 cagccatagt tataccttca ccactccaac caaatgaacc aaatactcct gctatcttgc
    22441 cttggttagc cattgggtct ataacagaga ataaatccca cataggttta accatagttt
    22501 tgtttatagt tggagaacct agtaatatta cttttgacac tactaatgta tcgtgcattt
    22561 ctgttaatgt catatcttct aaatcatata attcagcctt tgcaccttct ttatcaagtc
    22621 cttcttttat ctttttagcc atctctaaag tattactata agctgataag tagaatatag
    22681 aaacttgatt ttgattagtt gtattaacag cttctgtaga ccactcaaca tatcttttaa
    22741 cagcagccat tgggtcttta gtaagcattg gaccatgaga agtcaatata gtatcaaatt
    22801 ctatgtttag tcctaccacc ttatctacag cacttaaaac atgtttagca aatggcttaa
    22861 ctatacaatc atagtagtgt ttagctgatt ttagataatc ttcactattc actacctctg
    22921 catcaacact tgcaaagtga caaccaaatg catcacaagt taaaagagtc ttgtcctcct
    22981 caatgtaagt aaacattgta tcaacccaat gtaagaatgg agcagttata aatctaagat
    23041 ttcttttacc gatatttaaa atttcaccat cttttattac atgacagtta aaaggtctat
    23101 ttatttgacc atctaagtat agtttagcag ctttagtaca ataaacttct atattaggat
    23161 ttatatctaa taagtatttt aaactacctg catggtctgg ttctgtgtga tgaattacaa
    23221 catagtcaat tttagctata tcagtaacct ctgataaatt acttaaaaac tcatctttaa
    23281 aatttgcctt tacagtgtca aataaaaccg ttttttcgtc cttgattaaa taagagttgt
    23341 aagttgtccc aaactcagtt tccattatta tgtcgaatac ttttaaacct tcatctacta
    23401 cgccagtaaa gtagatatct ttttttactt caaaaacttt actcattttt aaccctccta
    23461 aaatttatca ttatataagt aacataccca attttgaggt gaatgaaaca aaagatg
//
LOCUS       DACTGW010000043        23321 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.043, whole genome shotgun sequence.
ACCESSION   DACTGW010000043 DACTGW010000000
VERSION     DACTGW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.043"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..663
                     /locus_tag="I9573_001319"
     CDS             <1..663
                     /locus_tag="I9573_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT4795946.1"
                     /translation="KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGG
                     SAPGNSSGGMIEKDYNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKP
                     DLFTSIHYNGSTNKQGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTR
                     VLPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDS
                     LK"
     gene            891..2804
                     /locus_tag="I9573_001320"
     CDS             891..2804
                     /locus_tag="I9573_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795947.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTDNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEINTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEENVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLRMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            2797..5016
                     /locus_tag="I9573_001321"
     CDS             2797..5016
                     /locus_tag="I9573_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAT4795948.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEIALEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLISENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            5125..5955
                     /locus_tag="I9573_001322"
     CDS             5125..5955
                     /locus_tag="I9573_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HAT4795949.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            5945..9412
                     /gene="addB"
                     /locus_tag="I9573_001323"
     CDS             5945..9412
                     /gene="addB"
                     /locus_tag="I9573_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HAT4795950.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIEN
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDDYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSTICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            9412..13239
                     /gene="addA"
                     /locus_tag="I9573_001324"
     CDS             9412..13239
                     /gene="addA"
                     /locus_tag="I9573_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAT4795951.1"
                     /translation="MSSPKWTKEQLEVIGSRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIANISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDIDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNETESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDSPDSDY
                     YSEIEKKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            13285..14496
                     /locus_tag="I9573_001325"
     CDS             13285..14496
                     /locus_tag="I9573_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAT4795952.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVIATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            14483..18010
                     /locus_tag="I9573_001326"
     CDS             14483..18010
                     /locus_tag="I9573_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4795953.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNIQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFVVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKDLKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRS
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVINITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            18141..19394
                     /locus_tag="I9573_001327"
     CDS             18141..19394
                     /locus_tag="I9573_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAT4795954.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            19543..20595
                     /gene="ytvI"
                     /locus_tag="I9573_001328"
     CDS             19543..20595
                     /gene="ytvI"
                     /locus_tag="I9573_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HAT4795955.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            20686..21912
                     /gene="pepT"
                     /locus_tag="I9573_001329"
     CDS             20686..21912
                     /gene="pepT"
                     /locus_tag="I9573_001329"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAT4795956.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            22119..23171
                     /locus_tag="I9573_001330"
     CDS             22119..23171
                     /locus_tag="I9573_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HAT4795957.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT         9963 a         2181 c         4158 g         7019 t
ORIGIN      
        1 aaaataggtg gaggattgac ttctaaggta atgagctcta tagcatcatc attatctaaa
       61 cacaatacga ctccaacaga accaggaaat agtgggggca agacagttat gattgaccca
      121 gggcatggtg gttcagcacc tggaaattca tctggaggaa tgattgaaaa agattacaat
      181 ttaaatactt cacttgcaac aactgaatat ttacgttcaa agggattcaa tgtaataatg
      241 acaagagaca cagataagac tttatctctt ggaaatagaa ctgctctatc taattcattg
      301 aaaccagatt tatttacaag tatacattat aatggctcaa ctaataaaca aggtcatggt
      361 gtagaagtat tttataagct taaagataaa aatgggggga ctactaaaac tgtagctacc
      421 aatatattaa atagaatttt agagaaattt aaacttacaa atagaggtat aaaaacaaga
      481 gtacttccta gtgattctac aaaagattat ttatacgttt taagaagtaa tgatatgcca
      541 gctgtacttg tagaatgtgc atttttggat aatgaaaatg atatgagttt aataaactca
      601 tctgcaaaag taaaagaaat gggtacacaa ataggtaaag gaatagaaga ttcattaaaa
      661 taaatggaaa taaaaatatt taaaaactta atgtaaattt atactaaagt caaaaaatga
      721 aatataagtg tatcaatagt aagactgtat gtacatttgc acatgtgaat gcaagtatat
      781 atacagtctt tttttgtagt taaatgcaat aatttgattg tgttttgtaa aatacacaat
      841 atgatataat tagataatat acacttaatt taattgagga aggtgattat atggaatatt
      901 tatttgaaaa aagtagttta aaagaatatg gaaatgaagc tttagataaa tatagagaat
      961 tattagataa aactgataat aattcaaggt gtattctttt attagtacca aacaataaca
     1021 caaagatacg atatgaaagg gctctaagat taaattatag tgaagaactg aaaataacaa
     1081 catatataag ttttgtgaag aaagaattag ttaaatactg gccactaata atagaaaaat
     1141 gtgatgaaat aaatacaaag gttgtatctc caacatttat atcaaatagt ttaagtgaat
     1201 atttaatagt gcaaaaagtt aaggaaaaaa ggatacaaga aggatatttt gaagatatta
     1261 caggaaccaa taaatccata gcaaatagta taatgactaa cataaataaa tctgcattta
     1321 atttaattga ttttgatttt attggagaaa aaatatattc atcaaagaaa aataaagata
     1381 gtatatatag attttcatac actcagatgg atgaaattat atcatattat ataaattcgt
     1441 tactatcaaa ttcaatttta gataatgcat taagcatata tttatacaat caatacctac
     1501 ttaatgatga tttttattta aaaaacttat ttacagaagt gagatatata attgtggata
     1561 gtcttgaaag ctcaagtaat gcagaagttg attttataac agaagcattg gataatacat
     1621 tacaaagtta tatatatttt gattatagta gggattattc tgtatttaat aatgtggata
     1681 tagactatat aaatgagaaa ataatatcaa aaattaagcc taaagaagaa aatattaaaa
     1741 agaaggagat tagaatagaa gatttatact tattacctgc aaatatagaa ttaaatcaat
     1801 caagtcaatt atacaatgaa atgttagatt taataagtga aaaagttata tctcttatag
     1861 aagaaaatgt atctccaaga gatatagcaa ttatatctcc tataaacaat agtatattgg
     1921 agcatcaaat tagagattct ttgatagaaa aaaatataga tgtttttagt actaagaagg
     1981 ataaaaaagc tgtagattat ccatatggga atgcattggt agtggctaca tgtatatttt
     2041 atggatattt agattttata aaagatgatg aatacttaag ctttttagaa actcttttag
     2101 aaataaatag gataaaagca tttaagattt ttaatgaaac aagacattta agtattgata
     2161 atatagatga acatagtcag tatcgtgata ttttacagta tattgaagaa aaaaagaaga
     2221 gtgatataaa aatacatgaa tttttaactc aattttacat agataaaatg ttaaatttaa
     2281 aagaaggtaa aaagaatgtt gggttatgta aaaagataat agctgaaagt gagtctttta
     2341 gtgaaagttt aagtctactt aggatgaaaa aagaaaagat atttatagaa gctttaaaaa
     2401 gtacaataaa cgattattac tctgttgtcg atatagaaga gttgaaatcc aaagacaaaa
     2461 tagtaataac tactccttat tcatatatat catcaaatat agatagaagt atacaaatat
     2521 gggtagatat aggaagtaat gcttggaata tgaaaataga aaaagatata agtaatttaa
     2581 ttgtacttag aaaatctttt gaagaaaaaa agatttatac aaatgaaatg gaagaatatt
     2641 ataaaaaata ttatctatac aatacagtat ataatttgtt attaaatgca aagaaaatct
     2701 atgcatttaa aagtgaatat gcgattaatg gttatatcca agagagtata ttatatagta
     2761 tacttttaaa aatctcacat aaaggagata aaaattatga ttaatataaa ttatagagaa
     2821 gaccagatac caataataaa ttacgataat ggtactatgg cagtaccagc agtaccaggt
     2881 gctggaaaaa catttataat aacaaattta gtagctaaac tattacttga acagaaacat
     2941 aagggtggta aaatattaat tcttacatat atgaatagtg ctgtaaataa ttttaaaggt
     3001 agaataagaa agattcttga agagaataaa atagatgata ctaattcgta tgaagtaatg
     3061 actatacaca gtttggctgt aaaaataata aaggaaaagc cagaagttgt aatgttaagt
     3121 gaggatttta acatagctga cgatttgcaa aaaagtatta tattaaatga atgtataagt
     3181 agatttagat ttgaaggtgg agaaagagct tttagatggt ttttaaagga gcaaaaagat
     3241 gaacgctgga aagaaatagc tcttgaagcc tgggaaagag gtttttttga atttgcagga
     3301 aatgcaataa gtgaacttaa gtataaggga atttctccag ataaacttga agaaatattg
     3361 tcaagaggac acaaaggaat gcttaaaatt attttaccaa tatataaatt gtatgataga
     3421 aaattaaagc aaaatggatt gctagattat gatgatatat taattttagc agaaaaaact
     3481 ttaagcttag acgaagggct tagaaagaag tttcaaacta gatacaaata tatatttgaa
     3541 gatgagtgtc aggactcaaa tgaaattcaa ggtaatataa taaaaattat atctagtgaa
     3601 aataaaaacc tagttagagt tggagatata aatcagagta taacaggaac attttcatca
     3661 tcagacccta aattttttaa agaatttata aatagtgcag atttctgtta taggatggat
     3721 atgtcaaatc gaagttcaaa agatatactt gatttagcaa acactcttgt caagtatgtt
     3781 acaaaggaat ttagacaaga agaatgtaga catgctcttg aagatatgca aataaaaact
     3841 gtgccaaatg gaatgggata taaagaaaat cctaatccag aacattataa tataaatgta
     3901 aagtggtatg agagttggaa aattgaaata gagcaaactg ctagatacgt taagggtata
     3961 aaaaataaat atccagataa aagtataggt atattggttc catttaatga acaagtgaca
     4021 caagttgcta aagaacttag agagaataat ctaacctttg aagaattagg accaaattct
     4081 ttgaataaaa ggaaggttat aaacaacata gcatatatta ttgattttat gttacattgt
     4141 gatgatgtag aaaagttaat agttgtactt aataaagttt ttatagatac tgataatgaa
     4201 gaagaaataa agtatttttt agaagaatta aaaaactatt ctaccgaaga aattatttat
     4261 aattttgaat ttagtaagtt agataatgat ttaatagaat ttaaagaaga aagtgacata
     4321 tatttaagtt ttaaaagagg aatagatatt ataaaacaga tattagaata tccaataata
     4381 aggttagacc tcttagtttt gtttattggg aaaaaactta atttagaaaa agaagataaa
     4441 gctgtactgg attatatatc tttttatata aagtatctaa tatcagaaaa tataaatatg
     4501 gatttagaag atgtatataa tttattgttt gatgtaaaga atagggtgtt taatcatata
     4561 atagatgtgg tatatgagat aaatggatat gaaccagagc ccggtagtat aacactatgt
     4621 aactatcata agtcaaaggg gatggaatgg gattgtgtgt ttttactagg tcttgttgaa
     4681 tataatttcc ctgacaatat taatcaaaag tttcagagtg ataaatggta tctaaaagaa
     4741 aaatataaaa accctatggc aattattaaa tcagaagttg aagctatatt aaaaggaagt
     4801 atttcaacag attatgctca taaaacgaag attgattcta taaatgaaaa gattagactt
     4861 ttatatgtag ggattactag agcaaaggaa atgttagtac tttcaggaag tgcttataga
     4921 gatgaaagtg atatagggaa taagagaaaa gagcaaaaac catgtatata tttaagcaga
     4981 cttaatcaac atattattga aaaaaggtca aattaatata taataagtta ttaaaagatt
     5041 gcttgaaata tatctaaaag gggataaaac aatatgtaca gtaagtaact actaaaaaat
     5101 aaagttaaga gagaagtgat atgagtgaac gataaattaa aatatttttc ttatagtcaa
     5161 aattcaatca acacatataa atcatgtcca ttgaaattta aatataaata tattgataga
     5221 ataaattgga aaaatgatga tgttggtagt agagagtatt atgaaacttt aaaaacagga
     5281 agagattttc atttgatttg tgaaagatat tttagcaata tacctcttgg aatctatttt
     5341 aatgaggatg ataaaaatag taaaaagttt ttaaagtgga tggaaaatat aaaaaaagtt
     5401 gttcctattg aaaaaggaaa aacgtatttg ccagaatatg aagtaaggat gaccttaaat
     5461 ggggatataa tacaggcaaa gtatgattta gttgtaattg gaaataacaa tatagaaata
     5521 tgggattgga aaaccgagtc taaaaagatt gactataaac acgtagaaaa tagaatacaa
     5581 actattgttt atatgttttt agcaaaagaa gtaataccta aagttttaaa aatggacata
     5641 aatgttaaag atataaatat gagatattat caacctgaat ttgatgactt accaattacc
     5701 attttatatg atgaagaaaa acatgaagca aatagaaata aaattcaaaa ttatataagt
     5761 atgataaaaa acactaatta tgaagaacat acatacgaag acaatctata caatgatata
     5821 gaaaaggtat ataataaaga agaacgtatg tgctatagaa ataaaaagca ttgcaagtat
     5881 tgtgaattta ataagttatg taatggcaaa gaaatagatt atagtatttt ggaggcggaa
     5941 atttatggga cttaggtttg tattaggtag aagtggaagt ggaaaatcca cctatatact
     6001 agatgagatt aaaaaagaag ctcaaaaaaa tgaaacgaca tcaattatac ttttagttcc
     6061 agaacagtac acatttgagg ctgaaaatag agtaagtaag ctattcttag gaaaagaaaa
     6121 agataagtat ctaagagtta gggtacttag ctttaagaca ctaagtaata tagttttttc
     6181 tcaagtagga ggattgacag atgtaaatat aaattctagt ggaaaagcaa tgatggtata
     6241 tagagctatt gaagatgtaa gtgaagaatt aaatgtgttt tcaaaatcaa aatcgcagtc
     6301 tggttttgta agctcaatta cagatatgat atcagaaatg aaacaataca atataagccc
     6361 tgaaatgtta gaaaatatat ctggagaatt ggataatgaa actttaagtt taaaattaaa
     6421 agatataagt aaaatatata attcttttga agggaaattg catgaaaatt atgtagatgc
     6481 tcaagatatg ttaacttcat tggcaagtaa aattgaatta agcagttatt tagatggagc
     6541 ttgtgtttat atagatgaat ttacaggttt tacaccaaat cagtacaatg tcataaagag
     6601 tatattaaat aagtctaaaa gtgttaatat ctctttgact gtagatgata tcaactatat
     6661 tgggtacagt aaatctgata tgttttctag aacgaaattt acttattcaa aattgactca
     6721 attgtgcaac gaagaaggca taaaaatatt gccacaagta aatttaaaca caggtgttat
     6781 aaaaagattt gaaaaagtta aggaattaca gcacctagaa agattttata atgcttatcc
     6841 atataaaata tattcaaatc caactgaaaa tataaagata aaagagttta acaatttata
     6901 ttcagaggtg gaagagatag ccagagaaat tgtacattta gttagagata agaatgtaag
     6961 atatagagat ataaccattg ctacaagaga tttaaataga tatgattttt tagtacactc
     7021 tatatttaat gaatataata taccaaactt tatagataag aagagagagg caaaaagtaa
     7081 tccaatagtt attttaatta tttcagcttt agaaatgaag aatagacgat atgggtatga
     7141 aactatgttt aggtatttaa aaagtggact tataggaatt gataatgatg atataaattt
     7201 actagaaaat tatgtgttgg ctaatgggat taaaggtaaa aaatggtttg atgaaaaatg
     7261 ggattataga ataacacaaa gtctttcagg tcaagaaagt gaatttgaac tggaattaaa
     7321 agaaaaaata aatgaaataa aaaatagagt attagagcct atagtaattt tacaagaaaa
     7381 attaagagga aaaaatagag taaaagaaat ctgtagatat atatatgaat ttttattaga
     7441 tataaatatg ccagaaacta tagaaaattt aatagtaaat tttaaggata aaggggaact
     7501 tgatgttgca aatcaatact cacaagtttg ggacatagta gtggacatat tagaccaaat
     7561 ggtggaactt atgggtgatg aaataatttc tttagaaaaa tttataaaat taataacctt
     7621 aggctttgat gaatatgaat taggactggt tcctcctagt atagaccagg tacttgtaag
     7681 tagtgtggat agaatgaaaa atccagacac gaaatattta tacctagttg gaactactga
     7741 tggtgtgttt ccactgatta caaaggatag tggaatattg agtgataatg atagggaaag
     7801 tcttggaaac aaaggaatcg aagttgatat tgatagtaag actaggagtt ttgaagaaca
     7861 gtttcttgta tataaagctc ttacttcgac atctaagaat ttaaccataa catatccaat
     7921 atcagaccat gaaggtaaaa cgcttagacc atcaataatt atatcaagac ttaagaagat
     7981 atttccaaat atagaaaata aaagttattt agtggaagaa aataaaagca ctgataaaga
     8041 tatacttaaa aaaataacgg ttaaatcccc tacatttaat gaacttataa acgtaataaa
     8101 aaattatgac agtgatgatt acaatacaga agagattaat agtatttggt tagatatata
     8161 taggtactat ttaaaagatg aaatctatag ttctataaca aaaaaagtta taaaaggtct
     8221 tagttatact aatcaagttc ataagattga agagaaaaaa ataaggtcat tgtataaaag
     8281 taattctctt agcatatcta ggcttgaaaa atatgctgaa tgtccatttg cttattttat
     8341 acaatatggt ctaaaagcaa aaaagcgtaa agagtattca tttacaccac cagatttagg
     8401 aacttttatt cataatattt tagataggtt ttcaaaagaa cttttacaag ataatttaac
     8461 ttggagagat attgatgaaa aatatataga attaaagata ggtataatag ttgatgaaat
     8521 aattttaaag ataccaggat atatattaaa tagttctgag aggtataaat accttgcata
     8581 tagattaaag aatatgttga caactgctat aactataatt agtcagcaaa taaaacaagg
     8641 ttcatttgaa ccaatagatt atgaagttaa atttggcgac aatggaaaat atccacctat
     8701 aaaaatggtg ttagaaaatg gacaagaagt aagtttaatt ggacaaatag atagagttga
     8761 tgaatttgaa gaaggagaaa ataaatatat aagaataata gattataagt caggcaataa
     8821 gtccataagt ctaacagaaa tatattatgg tctacaatta caattacttg tgtatttaga
     8881 tgcaatcctt gaaagtgcaa aggatgaaga tataaatata aatccagctg caatactata
     8941 ttgtagaata aataatccaa tagcaaaatt taatgaagat aaagatgatg aagaaataca
     9001 agaagcaata ttaaaagaac ttagaatgaa ggggcttgta gtaaaagact ctcacatagt
     9061 taaagagatg gataaatcat taatagatgg agaaaggaaa aactctttag taataccagt
     9121 aggtcttact aaggatggaa atgtaggtaa aagtacttca gcaataagct atgaggattt
     9181 caaactgctt agaaaatatg taaggcatgc tataaaggat ttatgtgaag aaatgttaag
     9241 tggagaaata cgtatagctc catataaaca taaagatgga acatcatgtg acttttgtga
     9301 ttactctact atatgtcagt ttgattcaac tataaaagat aacaagtata aaaatttaaa
     9361 taataagagt aatgaagaga taataaaaat gatgaaagga gatgttaatt agtgagttct
     9421 cctaaatgga caaaagagca attagaagtt ataggaagta gagaatgcaa tttattggtt
     9481 gctgctgctg ctggttcagg aaaaacagcc gttctagttg agcgtataat tcaaatgata
     9541 acaagtagag aaaatccaat tgatatagat aagttattag ttgttacatt tactaatgct
     9601 gctgcgtcag aaatgagaga gaggataggg gatgctatag gaaaagcctt agatgaaaac
     9661 ccagaaaata aacatttaca aaaccaactt gttttattaa ataaatctag tattacaaca
     9721 atacactcat tctgtttgga tgttataaaa tcaaactttc atagaataaa cttagaccct
     9781 aatttcagaa taggagacca aactgaatgt gctattttga aacaagaagc aatagaggaa
     9841 gtatttgaag atttatatga agaaagagat gaggggtttt taaatttagt tgaaagttat
     9901 gcagaaagag gaggagataa agaagtacaa gatattatct tgggaatata ttcatttgca
     9961 atggcttctc ctgaacctaa aaaatggttg attgactcag cagaacgatt taatattgat
    10021 gaaaattttg atttttcaca gtctatttgg gctagagcta tattagacac tgtaaaaata
    10081 gaaattaatg gtctttgtct aaatatggaa agggcattaa aagaagtgga atcaatagaa
    10141 gaattagaaa cttttgcaga aaagttatcc gttgaatata aaaaaattgc taacatttca
    10201 caagcatgta ataaatcttg ggatgaggca tataaaaaga tggcaagtat gtcctttgaa
    10261 aattatgtaa aaggagtaaa aagaatttca aaagatgctc cttcgtatat aaaagaatca
    10321 aaagaaaagg caaaaactat aagagataag actaagaagt ctttagaaag tatagtaagt
    10381 gctacattca ataaagataa tgattctatt agagaagaaa taaaatatct ttataacata
    10441 gttaaaccaa tatctagtgt tgtactgaga tttgaagagg aatatagcaa taagaaaaga
    10501 gaaaaaggaa taatagattt taacgatata gagcattttg cattaaatat acttacagat
    10561 atagatgaaa aaggtaatat tgtaccttct gatatagccg ttggatatag aaacaagttt
    10621 tatgaaatat ttatagatga atatcaggat agtaatctag tgcaggaagt actgcttaaa
    10681 gctgttgcaa atacagaaac tccaaataga tttatggttg gtgatgtaaa acagagtata
    10741 tatagattta gacaagcaaa gccagaatta tttttgcaaa aatataataa ctataatgat
    10801 aaaaagggaa gttcccatag aaaaataatg ctttacaaaa attttagaag tagagaagaa
    10861 gttgtagatg ctgtaaatta tatatttgaa aatataatga atgagaatat aggagaaatt
    10921 gaatatacag aaaaagaaag actaaattta ggtgcgaatt ttaatgtgga tacagacgag
    10981 aaatctatta ttggtggagc aacggagata cacctaatac aaaaagataa taaacttgat
    11041 gatgatatta taaatgataa agacgataga attaataaca aagaaaatga aatagaggaa
    11101 gaagaaaaat tagataatat acaacttgag gctagaatgg ttggaaacat tatcaaagat
    11161 ttaatgaaag tcaatgaaga tggaaaaatt caaaaggtct atgataaggg aatagatgga
    11221 tatagacctg tggaatttag agatatagtt attcttttaa gagctacatc tgcatgggct
    11281 ccagtgtttg cagatgaatt gatgaatatg gatataccaa catatgctga tgttggagtg
    11341 gggtattttg atacgattga aataaaaaca atattgtcat tacttcaaat aatagataat
    11401 ccaatgcaag atatacctct tatatctgta ttaaaatcac ctatatttgg atttacacca
    11461 gaggatttga ttgatattag agttcagagt aaagataaaa ttttttatga ggttttaaaa
    11521 agtacagcag agtatgatgg atttacagat tctcaaaatg aaactgagtc tgagtttata
    11581 ccaagtgaag agtgcataaa caaaagtaaa gattttttaa ttaagctaaa ggaatttaaa
    11641 gaaaagtcaa tgtacatgag tacagatgaa tttatatggt acttgtacac aaggactgga
    11701 tactatgctt atgttggagc attgccaggt ggaagtcaaa gacaagctaa tttaaaagta
    11761 ttgtttgaga gagcaaaaca atttgaagaa accagtctta aaggaatatt caactttgta
    11821 aattttatag agaaattaaa aaaatctagt tctgatatgg gaagtgcaaa aacacttggt
    11881 gagaatgcaa atgttgttag aataatgagt attcataaaa gtaaaggtct agaatttcca
    11941 gtagtcatat gctctgcaat gggtaaaaat ttcaatactc aagattttaa aaagagtatt
    12001 ttatatcatc ataatttagg gtatggacct caatttgtag actatgagag aaggatttct
    12061 tttccaagta tagccaaaga agcattaaaa agcaaaataa atatagaaaa tttatcagaa
    12121 gagatgagag ttttatatgt tgcatttaca agagctaaag aaaaattgat tataactggt
    12181 tctactagaa atatacaaga tagtataaag agatggtcaa atggaattga aagtttagat
    12241 acaatatcac aatacgagat attaaaaggt aaaaattttt tagattggat aatgccttgt
    12301 gtattaagac atagggattt atctaatcta ctagaagaag taggattaga cgcagttttt
    12361 aatgttgaac ataattcaaa atggtatgga aagttgtgga ataagaatga tattttagtg
    12421 gaaaagaaga gtgatgaaga aaaagaaagt attgaagaaa ttcttgaaaa aatagatgta
    12481 gatagtccgg acagtgatta ttacagtgaa atagaaaaaa aattaaacta tatatatcca
    12541 tatgaattta gtacaagaaa acctgctact atatctgtta cagaaataaa aaagatacaa
    12601 aataattatg aagaagagtt aataaatact atatttgaac aaaaagtaat attaaaaaag
    12661 cctctattta ttcaaaatga agaagaaaga gaaaaaataa gtggaacaga aagaggtaca
    12721 atagtccatt tggttatgga ggttttagac ttaaaaaatg taagtagtgt aaatgatata
    12781 aaatcacaaa taagaggttt tgtttcaaaa ggtataataa cagaaaaaca agctagtata
    12841 gtaaatccat ataaaatata taaatttttt gcctctaaca taggaaaaag aatgttgaat
    12901 gctgaaatta taaatagaga aaaatcaatt tatgcacaag taaatatgaa agacatatat
    12961 atttatgaaa agcttataaa caatgatgat aaaaaacttt atgataatga aagtgtcatg
    13021 ttaagaggta tagttgatgc ttattttgaa gaagataatc aaatagtttt ggtggattat
    13081 aaaacagatt ttgtaaatga ggaaaatata aatcaaataa tagagaaata taaaaaacag
    13141 ttggatttat atgcagatat tattgaaact ttaactggta agtcagtaaa agagaaatgt
    13201 atatatttat ttggagttga tgaagctgtt tgttattagt ttttatatat aaattaaacc
    13261 taaaagatag aggaagggga aaaaatgaga tttattcata catcagattg gcatctaggg
    13321 aagtccctag aaggccattc tagaattgag gaacaagcaa agttttgtga ggaatttata
    13381 aaaatagtag aatcaaatga aatagatatg gttataattg ctggagatgt atatgatact
    13441 tctaatcccc cagcacaagc agaaaaacta ttttatcaga ctgtatcaag acttgccaat
    13501 aatggtcaga ggtgtgtatt aataatttca ggaaatcatg ataatccaga gagactttct
    13561 gcaataacac ctctagcaca tgaacagggg atacttatat atggttatcc attaagtgct
    13621 acgatagaag ctaagtataa agggtttgaa ataacatatg caactcaggg atgtacaaaa
    13681 ctgaatataa atggagaaaa tattgtgatt gctacattac cttatccaag tgaaaaaaga
    13741 ttaaatgaag ttttttcaag tgaagatgag tttgagaaac aaaaaaatta ttctgaaaag
    13801 gtaggagata tatttagaag tttagaagaa aattttagaa gtgatactat aaatatagcc
    13861 gtatcacata tatttgtaat aggtggagag agtactgaat cagaaagacc tatacaatta
    13921 ggtggaagtt ttttagttga aagaaaagat ttgccagaaa aggctcaata tactgcacta
    13981 ggtcatttac ataaacaaca aaaagcttcc gaacgtttaa atgcatacta ttctggttct
    14041 ccacttcaat atagcaagga tgaaagagcg tataccaaag gggcatacat agtggatata
    14101 aaagctggag aaaaaccaat aattgaagat gtatatttta ataattacaa acctatagaa
    14161 gtatttaaat gtaatggaat agaagaagca ctagatatat gtgagcaaaa tcaagataga
    14221 gagatttggt catattttga aataaatact gatgagataa tatcacagaa tgaaattaaa
    14281 aaaatgaagg aactattaaa agacataata gaaataaaac caataataac atcttgttat
    14341 gaacaagaaa gtgtagatat aaaagaaaag tctatggcag agttgtttag agagttctat
    14401 tcttttagta aaggtgtaga gccaaaagga gaacttatgg atttatttct agatataata
    14461 agtgaagaag gtgaatcagc agatgagacc aattagatta gaacttacag gacttaatag
    14521 ctatattgat aaacaagtta tagactttga aaaattaatt gaaagaggtt tgtttgggat
    14581 atttggaact actggaagtg gaaaatctac aatcttagat gcaataacta ttgctatgta
    14641 tggaaatata tccagaaata caaaagaata tataaacagt gtgtgcgata aggcaattat
    14701 atcatacgag tttgagatag gaagcaaaaa cactagaaga agatacattg tagatagaac
    14761 cattgctaga agcaaaacag gaacaaagac ctctaatgct agattaatag aagtattgaa
    14821 tgacaatatc caaaatgttt tagctgataa agttgtggaa gtaaatgaaa aagtagcaca
    14881 ggttgtagga ttaacggcaa atgattttac taggtcagta gttttaccac aagacaaatt
    14941 taatgagttt ctaagattat cacgtgcaga tagaagagac atgttagaga gaatttttaa
    15001 tcttgagaaa tatggaagaa gtcttggaga aaaagtgaaa aagagaaaaa atatacagtt
    15061 acaaaattta aaagatttga aatcaaaatt aagtcaatat gatggaatta cagaggaagt
    15121 atacaacaat ataaaccaag aattaataga gttaaaaaat ttggaaaaag ataagaataa
    15181 tgctttagac ctagctcaaa aatcttatga agaaagtaaa acagtatatg aagaacaatt
    15241 aaagttagaa aaaaatgaac ttagaaaaaa tgagcttgat ttaaaaaata gtgagataaa
    15301 agaaaagatt gctcttgtag aaaatgatag taacgctaaa aaggtagacc catacataag
    15361 ttcagttcaa aatctagaaa aaaagataga tgaagatagc tttgtagtaa gtaatttaga
    15421 aaagaaatta gcaatattaa atcaggagct tgaagttaca aaaaatagat atgaaaaaat
    15481 aaacaaaatt aagaatgaag aagtccctaa attgagtgaa gaaaaaataa gattgcaaca
    15541 agcaataaaa ttagaagaag agttagtttt attagataga gagttaaaag atctaaaaga
    15601 aagtggaatt aatttaaata agactaaagt tgaattagaa aaagtaaagc aggtgtcaga
    15661 gtccagaaaa gatgcagtta ctaaatctat aaaagaagtt gaaggaaaga ttgataaagt
    15721 aaatataagt gcagaattaa aacaaaaaat atttctagca tatgaatacg agaaagatta
    15781 caacaacgta ttggaagaaa aaaatcaaaa gctaaataaa ctagaagaaa tactcaaaga
    15841 gacagaaaat ataaatttaa aagttagata tattgataaa gataagaatg atgtaaatcg
    15901 taatttagaa aatttatcac ttcacttgga tgttcttctt aaaaaatgtc ctggtaagag
    15961 tgctgattta cttttaaaat ctgaatatgt tactgaatta agaagtaaag caaataatac
    16021 taaagaaaat gaaattaaga aaagcagtat acaagatgaa ttaaagataa tacttgaaag
    16081 taagtttaat actgaaaggg aagtaaattt actaaatgaa aaacttgaaa ataatagaaa
    16141 gaatagagat gacctagaaa aagaattaga agaactaaag tatttaaatt tagcctctga
    16201 acttagacgt gaattaaaag aaaatatgcc ttgtccagtt tgtgggtcaa aacatcatga
    16261 aaatcataat ataactaagt atgatgaaaa tatatctttt gtcaaagaaa aactggagaa
    16321 attagaaaaa gaaaaaataa gtataagaaa caatatagag gaattaaatg ctaaagtaag
    16381 tgggtattta tctatagaaa aaatgaagac taaagaactt gaagatgtaa aaggaaaact
    16441 aggggggatt ccatctagtc aattattaaa aaaacttgat gaagaacaaa gaaaacttgc
    16501 attgttaaaa tctaatattc aggagtggga gaaagaaaaa gagagtactg aaaataaagt
    16561 aacattagct aaagaagaaa aaaataaaat tgaaaaagaa gaattaaaaa taagagagag
    16621 tctgaataat tataagaaat taacaagaga tttaaacata gaaatagaag gtttagaaaa
    16681 taaatgtaaa aaacttaaac aggaatacct aggattaaag acgattacta aagtctctaa
    16741 tctattgtca aaagtaaaag aaataaggga gaatgaaaaa gaattagaat tactaagtac
    16801 tgggcattca aatttattaa aaaatagaga ctcactagat attaaaataa gagaaaatga
    16861 gagtaaacta catgaaatag agatagaatt aataaaggct agagaattat atgtagaaaa
    16921 gaaattgagt agagataata agtataaaga agttattaac ataacgaaag gtgatttagc
    16981 taaaaatctt ttacataatg ttgaagaaaa tatatgtaag atattagaac aagaagaatc
    17041 aagtaagaaa aaattggaag agcaaagatt agaatatgaa aaaaacattg ctgaaaagca
    17101 caatatagat ggtagactta aaactgctaa ggaacaatat aaagaccaaa aatcaacact
    17161 aaataaacta ttagcagaaa ataaatttga aagtatatat gcagtaaaaa gagctttatt
    17221 agatactgat gcagtaaaaa aattgattga agaaatacta gagcatgaag aagaacaaaa
    17281 gttattatca tttaagataa aatcatcaaa agaaaaatta aatggaagaa gcataaagaa
    17341 ggagtacttt gaacagttaa aagatgaaat ttataatttg aaagtagaaa taggaaatat
    17401 atccaaagat attggagcta atcaaaatca gttaataaca ctaaaagact ctttagataa
    17461 gataaatgat tttaataaac aacttaaggt agttgaacac aatgtggact tattggaaga
    17521 attagataaa tgtgtgcaag gaaataaatt tgtagaatat gtttctacaa accaattaaa
    17581 atatatagca ttggaagcat caaaacgatt ggatggaata acaaaaggaa gatatgcact
    17641 tgaaattgac tcaacactaa actttgtaat gagagataac tttaatggag gagaaagaag
    17701 aagcattgat actctatctg gtggagagac attcttgaca tctctatcat tagcacttgc
    17761 tctttcatct caaatacaat taaagggaag tgcaccatta gaatttttct ttttagatga
    17821 aggttttggt tcattagata gtgaactttt agaaattgtt atagaatcct tggaaagatt
    17881 gcatagtaat aatctaagtg tgggtataat aagtcatgtc gaggaactta aaaatagagt
    17941 tcctgttaag ctattagttt catctagtga ggctggaata ggttcaaaag taaaaattga
    18001 atatagttag aaaatatatg gtaattgaat atattatcta atatttccaa ataatatgta
    18061 attgtgatat aataaaaact aatattaaag ttatttataa ttaatattaa tataaaaaca
    18121 tgtaagtagg agtgtggtaa gtgttggaat cgcccaataa tttgattcag attatttttt
    18181 taatagtatt acttataggg tctgcatttt tctcagcatc tgaaacggcc ttaatgtcgt
    18241 tgagtaaaat cagaatcaga tatatgcaag atgaaggagt taaaggagct aagttagtaa
    18301 gttcattaat agaaaatcca aataagcttt taagttctat attggttgga aataacgtcg
    18361 taaatatagc agcaacatcc atatcaacat ctttgtttat aggattaatg ggagaaaaag
    18421 gagtggcact tgctacagct gttatgacag tattagtatt aatatttggt gagattactc
    18481 caaaaacaat agcagcaaat aattcagaaa aggtttctct attagtatct aaacctataa
    18541 aagcaattat atttatatta agaccaatag tatggatatt taatataatt acaaatatta
    18601 tatttaaatt atttggtata acaaataaag gagctaaatc attcataact gaagaagagc
    18661 taaaaactat ggtaaatgta agtcatgaag aaggcgtcct tgaaatggaa gagagagaaa
    18721 tcataaataa tgtttttgaa tttggagata tgcaagctaa aaatgcaatg gtacaaagaa
    18781 tagatatggt agctatagat atggaagata gttatgatga gataatacag gtatttaaaa
    18841 ctgaaaaatt aagtagaatg cctgtgtatg aagaaactat agatgatatt gtaggaatac
    18901 ttaatataaa agatattata tttttatctg atgaagaaat agagtcattt gatattaaaa
    18961 attatatgag agaacctttt ttcacgtatg agttcaaaaa gataacacaa cttcttgaag
    19021 agatgaaact tgaaaaaagt cagatggcta tagttgtaga tgagtatggt ggaacatctg
    19081 gacttttaac tatagaagat ttagtagaag tcattgttgg agatatagag gatgaatatg
    19141 atgaagaaga ggatgaaata caagttataa aagaagatga gtatattgta gatggaagta
    19201 ctaaaattgg tgatgttaat gaacttattg gtgtaaactt ggaatctgag gagtttgatt
    19261 ccataggagg ttttataatt ggacacctaa gcagattgcc agaagaaaat gaagtaattg
    19321 aagttgataa tataagattt tgtatagaaa gtatagaaaa aaatagaatt aaaaaaataa
    19381 gaatatatac ataatgtaat aatgttctta aatttattag aagtgtataa atataaaaga
    19441 tatcttttat atttatacac ttctttttat tttgtattca aataaaacta gtatgaaatg
    19501 gacaaaataa aaatataaat tagaagaaag gagattttta agatgagcat actagaaaat
    19561 aattttttat acaaattaaa aaacaacact atcttttttt gtatatatac attgttattt
    19621 atttttattt acaaagcgtt tccatacata gctccatttt ttctaggtgc tataatagca
    19681 cttatgatta atcctatatc acaaaagtta gaaaataagt ttcgtataaa taaaggtatc
    19741 tctactcttg ttctaagttt tcttgcagtg gctatagtca gtacagttac tacaataata
    19801 gtaataaatt caatgaaaga attaatggga tttttaaata atatatctgc taatcctgag
    19861 gatataagta atactattat gtatttttta aataaaataa atgactttat gaaatcattt
    19921 caagaaatag ccaattttga tgtagaacaa ttagtaaaca aattcagtgg tgaagtaatg
    19981 caaattacaa aaaatcttct tactagtata cttggattgg ctacttctat tccatatatt
    20041 ataatattta taattacctt gtttattgct acctacttta tagcaaaaga ccttgataag
    20101 attgaaaata gtttttataa tatgtttact gttgatgtta gaaagaaggt aaaaaacgta
    20161 aagaaagagg cagggctttc acttgttgga tatataaggg cctatacaat acttatggct
    20221 ataacatttt ttgcaatctg gggaagtttt gctttatttg gattgaaata cggtctaata
    20281 gtagggtttg taggagcttt aatggattta attccattcc taggtataat aacaatatat
    20341 cttccagtaa ttgtttatta ttttcttata aaaaattatt ttatagctat aagtatgact
    20401 gtgatatttt ttgttttgtc tttgattaga gaaatacttg aacctaaact agtatcagta
    20461 aatgttggac taaatccatt agctacccta gcagcgattt ttattggaat ccaagtaaaa
    20521 ggaataattg gagtaatttt ttgtttgggt ttggtatgta tgcatgatat acttaagaag
    20581 gtggatattt tttaattata ataattgata gattttttta aaaataaaat tttattcaca
    20641 ggtaaataaa aaaatattaa tcttattaag aaagagggta tatacgtgaa acaaaaagta
    20701 gtggaaagat ttttaaaata tgtatcattt gatacaacat ctaattctca gtgtgagaac
    20761 tgtccatctt cagaaggtca aagagtttta gctaagtata tagttgaaga gctaaaaaca
    20821 atgggtgttg atgatgtgag tttagatgaa aatagttata taatggctac tttaaaagga
    20881 aatactgatg gtgtggatac tataggattc atatcacatt tagatacaat tgaagatgtt
    20941 agtggaaaag atattaaacc aagaataata gaaaattatg acggaaaaga catagtttta
    21001 aatgaagcgt taaatgttat tacgtatgta aaggattctc cagagttgga agaatttaaa
    21061 ggtgacgatt taatagtcac tgatggaaca actctacttg gctctgatga taaagctggt
    21121 atagctgaga tagtgactgc aatagagtat ctaataaatc atccagaaat taagcatgga
    21181 gatataaaaa taggatttac accagatgaa gaaattggta gaggtgctga tttatttgat
    21241 gttgaaaaat ttggagcaaa atatgcatat actttagatg gaggaatagt tggagaactt
    21301 gaatgtgaaa attttaatgc tgcaaatgca actattacta tacatggtag aaatgttcat
    21361 ccaggttcag ctaaaaataa aatggtaaat gctatacata tagctgctga aatatcagaa
    21421 atgttcccag cagatgaaag accagaaaca acagaaggat atgaaggatt ttggcattta
    21481 aattctatag gtggaaatgt cgagaatgta agtatggctt atattattag agaccattgt
    21541 agagaaaaat ttgaaaatag aaaatctatt atgatagaga atatagaaaa aataaataag
    21601 aaatacgata atagagtgga attggattta aaagattctt attacaatat gaaagaaaag
    21661 attgagccag ttatgtttat agttgatata gctaaagaag ctatggagga attaggtata
    21721 aaaccacgtt tagttcctgt aagaggtggt actgatggag caagattatc ttttaatgga
    21781 ttaccttgtc caaatatatt tactggtgga ttaaacttcc atgggaaaaa tgagtgtata
    21841 ccagttagtt ctatggagaa agctactaaa ctaattgtaa gaatagctga aaaatatgct
    21901 gaaagagttt agttaaaaag tatttaaaaa ttaagcattt aaagttataa tagctatatc
    21961 attctttata agtcaagctg attataagct tgtgttgtat ttaaaatggt atagctattt
    22021 ttgtaattat ttttataaaa gataattatt tttagtttag ggtatcaata aaaagtatgg
    22081 atatcaatag gtattaacat ttaaaagggg gaattgtatt gaaaattaaa tctaaaaaaa
    22141 taatagcatt tgcactttca cttgggatta tgttgccaag ttgtgtatat gcagacacta
    22201 ttagtaaaaa tcaaaatgca caggtagata tgaacaatta tcttcaaggt agaaaatctc
    22261 caaaggagga aaaacttatt ggtgaagata gatttgatac agcgataaaa atatctcaaa
    22321 gtggttggaa taatggttca gaaagggtgt ttctagttaa ctcaaattca ttgccagatg
    22381 cattggcttc aactcctttg gcaagtaaat tggatgcacc aatactgctt acaaataaaa
    22441 atagtatacc atataatgta acagaggaaa taaaaaggct taatccagca gaagttattt
    22501 taattggaag tgaaggagct atttcatcaa gtgtaaaaga atctcttgaa gatatggaag
    22561 tattagtcag tagaatcggt ggagcagata gagaagaaac atctttactt cttactagac
    22621 agctagatga tactggtgat ttaggtgtct caaaagtggc agtggttaat gggtataatg
    22681 gactggcaga tgcaacttca atatcaagcc cagctgcaag tgataataca gctataatat
    22741 atacaggtaa ggatagcata agaagtgagg caaagaattt tattacacaa aactcaacta
    22801 agacatacat agtaggtggg gagtacagta taagtaaaaa acttgaagga cagttagtaa
    22861 actcagaaag actagcagga acagatagaa aagatactaa tgcgaaagtt ttagaaaagt
    22921 tttatggaaa atcaagtaag gttaataata tgtattttgc aaaagatgga agtggtagag
    22981 aagctgattt agttgatggg cttgctgctg gagtattagc atcaaaaaca aagtcacctg
    23041 tagtacttgc aagtggaagt ttaagtagtg cacagaaaag ttttataaaa aaagtaaagg
    23101 ctgataaatt tgtacaagtt ggtggaggta aaaatagtaa accatatgca gaagcattag
    23161 ccttacagta atattgtgta aatcaaaatt aatatataat aatagcaagt aaaccaagtt
    23221 gaacttcaat atatataata aaataagtaa actaagttat atttcagtat aatatataat
    23281 gaaataagta aactaagcta tgtttaaata taatatataa t
//
LOCUS       DACTGW010000044        22978 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.044, whole genome shotgun sequence.
ACCESSION   DACTGW010000044 DACTGW010000000
VERSION     DACTGW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.044"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            163..1830
                     /locus_tag="I9573_001331"
     CDS             163..1830
                     /locus_tag="I9573_001331"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAT4795958.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNLENEKVREECYKVLEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(1926..3245)
                     /locus_tag="I9573_001332"
     CDS             complement(1926..3245)
                     /locus_tag="I9573_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT4795959.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYADKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(3286..4032)
                     /locus_tag="I9573_001333"
     CDS             complement(3286..4032)
                     /locus_tag="I9573_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAT4795960.1"
                     /translation="MKKLVKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(4276..6285)
                     /locus_tag="I9573_001334"
     CDS             complement(4276..6285)
                     /locus_tag="I9573_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4795961.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEEIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQSFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQGVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYSITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(6723..8156)
                     /locus_tag="I9573_001335"
     CDS             complement(6723..8156)
                     /locus_tag="I9573_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAT4795962.1"
                     /translation="MVLRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSREVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMTKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(8243..10159)
                     /locus_tag="I9573_001336"
     CDS             complement(8243..10159)
                     /locus_tag="I9573_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAT4795963.1"
                     /translation="MRRYEQLSKEIIENVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASIGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVIIIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGVLTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTSVVAAMYFKLKDKKIKELCIPAVISGICGVIEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDSIVEISKDNNGQGVVEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTIMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKHFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIIENTQ
                     KHVNNSQELITVLA"
     gene            complement(10233..11093)
                     /locus_tag="I9573_001337"
     CDS             complement(10233..11093)
                     /locus_tag="I9573_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAT4795964.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKCIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(11444..12631)
                     /locus_tag="I9573_001338"
     CDS             complement(11444..12631)
                     /locus_tag="I9573_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAT4795965.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGDNKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIESGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(12645..14036)
                     /locus_tag="I9573_001339"
     CDS             complement(12645..14036)
                     /locus_tag="I9573_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAT4795966.1"
                     /translation="MSSLKKKKFTMPQTLTIIFSLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMVFLTIGLYLGF"
     gene            complement(14631..16331)
                     /locus_tag="I9573_001340"
     CDS             complement(14631..16331)
                     /locus_tag="I9573_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAT4795967.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLASNGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(16303..17706)
                     /locus_tag="I9573_001341"
     CDS             complement(16303..17706)
                     /locus_tag="I9573_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAT4795968.1"
                     /translation="MIVNKELIIDYLKSNQENIIKDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGENGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTDDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(17970..18461)
                     /locus_tag="I9573_001342"
     CDS             complement(17970..18461)
                     /locus_tag="I9573_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAT4795969.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPNEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(18487..19299)
                     /locus_tag="I9573_001343"
     CDS             complement(18487..19299)
                     /locus_tag="I9573_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAT4795970.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLMGYEYKKVLSKEAIKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(19312..20463)
                     /locus_tag="I9573_001344"
     CDS             complement(19312..20463)
                     /locus_tag="I9573_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAT4795971.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            20733..21374
                     /locus_tag="I9573_001345"
     CDS             20733..21374
                     /locus_tag="I9573_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAT4795972.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CTNLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
     gene            21400..22944
                     /locus_tag="I9573_001346"
     CDS             21400..22944
                     /locus_tag="I9573_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT4795973.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AVFELATVNKSYTELKEILFSNSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
BASE COUNT         7535 a         4125 c         2550 g         8768 t
ORIGIN      
        1 gtttagacac tttatcccat actaacctca ctattctata tacaattata tgtttataca
       61 aataattatt tattgtatat tttagcttta atattagttt aatacttgac aatttgttta
      121 aacatattat aattaactta taaattacag gaggtatcaa ttatgaaaaa ttggtggaaa
      181 aaagcaactg tctatcaaat atatccaaaa agtttcaaag atagcaacaa tgatggtata
      241 ggtgatataa atggtattat agaaaaatta gattatctgt attctttagg tgttgattta
      301 ttatggttga ctcctatgta cgtttctcct caaagagata atggatatga tatagaagat
      361 tattataata tagacccaaa atatgggaca atgagtgact ttgaaaaact cttaaaagaa
      421 gctcataaaa gagacataaa aataatgatg gatatggtcc taaaccatac atctactgaa
      481 cataaatggt ttaaagaatc taaaaaaagc aaagacaatc catatcgtga ttattatttc
      541 tggaaagatg ctaaacctga tggttctgtt cctaataact ggatatccag atttagtgga
      601 actgcttgga aatatgatga aactactaac caatattatt tacatttatt tgaagaaact
      661 caagctgatt taaatttgga aaatgaaaag gttcgtgaag aatgttacaa agtactagaa
      721 ttttgggctg ataaaggtat tgatggtttt cgtttagatg tagtcaacct actctccaaa
      781 acacctggat tacctgatga ccctataaca gggccaaaag gagatggaag aactcattac
      841 gctgatggtc ctcgtataca cgaatattta cataatatga atcaaaaagt tttcaaacct
      901 aaaaatatag ttactgtagg tgaaatgtca tctactacac ctgaagagtg tatcaactat
      961 actagagaaa atcgagaaga actatctatg gtttttagct tccatcatat gaaaacagat
     1021 tataaaggag gcgcaaaatg gactaatgaa atgttctctc tggataaatt aaaaaaagca
     1081 caatctgact ttcaatataa aatgtatgaa ggaaagggat ggaatgctct attttactct
     1141 aatcacgacc aacctagagc tttatctcga tttggagatg atagattttt tcgtcaagaa
     1201 agtgctaaga tgttagccat aacactattt ggtctacaag gtactacata catatatcaa
     1261 ggtgaagaaa taggtatgac aaatgcctac tttactaaaa tagatgaata tgatgatgct
     1321 gaaagtaaga atgcatacta tcatatgatt aaatctggta ttgatgaaaa ggaggcattg
     1381 cttatacttc aacaaaaatc cagagataat gcccgtactc ctatgcaatg ggataatact
     1441 atttataagg ggttttctaa tcataaaccg tggttaaaag ttaataatga caatattagt
     1501 gtagaaaatg agcttaatga ttctcgttca gtattttata cttatcaaag attaatcaaa
     1561 tatcgtaaac aatatgatat ttttacagaa ggtacttata gattgttaga tgaaaatcat
     1621 aaaaatctat ttgtttatga aagagagtat aaaaaccaaa atcttcttgt tataagtaat
     1681 tttactcatg ataatgtagt atataaatta cctgaaactt atttgaataa attaaactac
     1741 acaatacttg aaaccaatta cactagagat ataattgaaa ataaaatgga aataaaacct
     1801 tacgaaagta taatgatata ttataaataa agttatttat ataaaaattg gtataacacc
     1861 agggtgttat accaatttta ctaaattcaa aataacattt atagaatatc tatagattac
     1921 aataattatt tgacttctac atcattatat ttctttgaat atactttagt catatagaaa
     1981 tatataggaa ctacaattat catcccacct aatccccaaa ctatcttcat aggttcatca
     2041 atacctgctt ttatcagtaa taatatactt ataataactg caaatattgg tataattgct
     2101 cctccaggta ttctaaaatt tacatcttta tcagcatact tctttctcaa tattagtaca
     2161 gctaaacatg taggaatata ttgaataaat ctagatacaa cacttatttg agataatgtt
     2221 gtgaatgaac ccgaccatgc tattagtaaa gttcctatga cagatactat aattgctata
     2281 taaggagctc catttttatt agtttttctt atagacttag gcattaatcc ttgttcaacc
     2341 aaagcagcag cagaccttgg tgtaaatatg gatgatgcaa tatttatacc tccaacagat
     2401 actatagttc ctattgtaat tatataccca cctataggac ctagcatctt aatggcagca
     2461 tctgcaacag gagtagctga ccctgccaag ccattaccca acacccctat acttattccc
     2521 agtataagca tatatattac tgatactaca aacattacca ttaccaatgc tttaggtaca
     2581 tttttcttag ggttctccat atcttttgct gcaacagcta aagattcaaa tcctgtaaaa
     2641 gcataaaata aagttataga tactgctaca aaagctgatg acgcactttg acccttttgt
     2701 attattacaa atggggtaaa attagaaccc tttataaaga ataatcctac agctataaat
     2761 aaaactattg gtaataattt tcctatagtc atgatattat ttaagtgctt agattgtttc
     2821 attcccataa aatttataat cccaagtcct acaactaata taccaattat tatattttta
     2881 ataagtggtt ctgctgcttg tggccataca gatgatagtg ttacagcaaa aaaatttgcc
     2941 atagtagccc aagcaatcat actaactatc cacttcataa atccaacttc aaatccaaca
     3001 aaatctccaa atgcctcttt tgcataaaga taaggccctc catcagtatc aaaccaacta
     3061 cctgcttcag caaaacataa agcaatagaa acagctaaaa gcatacaaaa aactaatact
     3121 aatatacttg ctggacctat atctgtataa gcatcaccag gtaacccgaa tatacctgac
     3181 cctattattg cattgattcc taatagtaca atactccaaa aacctaactt aatgcctttt
     3241 tccattgtta ttccccccaa agccaactta atatatttat attttttatt tagatgcttc
     3301 tttaactatt gccataaata tttttttcat attatcataa tctctagtca tcatttctgg
     3361 atgccattgt atccctatta caaactcact tccttccttt tctatagctt caattaaacc
     3421 gtctttagat gtagctgaaa caatatatcc tggtgctact ttgttgacaa ctaagtgatg
     3481 aaaactatta actaaaactt ctttttctcc taatatttca tataattttg tcccctcctt
     3541 tatctgtact gtatgtgtag gtacattaga tagatgttgc tgattgtgtt taatataagc
     3601 accttcaatt aaagataaat cttgatacaa actacctcct tcagttacat ttataatttg
     3661 ttctcctcta catataccta aaactggttt tttcatatct aaagcatgtt ttaataattt
     3721 catatcaaaa ctatctctct ttggtgatat tgcccccaac ttattatgag gttcttcttt
     3781 ccatatgagt ggatttacat cttgtccccc agatagtata agcgcatcta tattagatac
     3841 ctgctccttt ataatctcat catcatatac aatcggtaca atatatggta tagctttaca
     3901 cataactact gattgtatat aatcattatt tacataagcc ctttcatagc caggaaacat
     3961 tcctccttca tcaattagta aattacctga tattcctatt attggatatt tctttttaac
     4021 caatttcttc accccttaac ttatttgtga acaataaaaa atgctacttc tcttaaagag
     4081 caagtagcat accaagattt tatttatttg aacattataa ttcattttag aaatactttt
     4141 tttgaataca agtaatataa ataactttat ttacttatat tttattttaa tcctgtttag
     4201 tttgactttt ttaaattgtt ttaagtacaa ctaataggta aaataggcat ttagattttc
     4261 ctttttatca acctattaaa tactgtttaa tatatttttt ccccttctat ttaattcact
     4321 tccctttcta ccagattcac atattatcaa atccagctct tttaacatat taagtattcc
     4381 ccttacctca ccttctgtta tactgtagcc taattcattt attgaattaa caataccatt
     4441 tctacctata tttttattaa gattattggc attttttaaa cactccatta ctaactttac
     4501 tttctctata gatgttctat cctttaatat gcttatttct ttactaaaat cttcagttat
     4561 atttaataag ttatgaggta aatcatttat taaaataaca tcctcagaac tcataatgtt
     4621 aatatacata gcagtatttt ttaattctct tatattacct ggccattcat atgcattaat
     4681 aatgtcttga acaccttgac taattgctct cttccttttc ataaaatgct ttaacataac
     4741 ttctatatct tcttttcgct ctcttaaagg tggtatattt atgggcaata catttatcct
     4801 atagtataaa tctgctctaa atctatctcc ttttatcatt tctaataaat ttttatttgt
     4861 agccgcaatt actctaacat ctatatctat tacattttct cctccaaccc tcattacttg
     4921 attctcttgt aatactctaa gtaatttgct ttgtaagtat ataggcatgt ctcctatttc
     4981 atccaaaaat atagttccat tgttagcctg ttcaaataat ccttttttac ctccttttaa
     5041 agctcctgta aaagcacctt cttcatatcc aaaaagttca ctttcaagta aattttctgg
     5101 catagctgca cagtttatcg ctataaatga ctgactatgt cttggtgatg cattatgaat
     5161 agactgagcc ataagttctt ttcctgttcc actttctccc gttataagaa ttgtataatc
     5221 agattcagct attttcatac ctaatttctt agttctttcc atcttcgttg aaactgtttt
     5281 tatatcatca aaagtatatc tagcaacttg tcccttttct ctaagcttct ttgataggtt
     5341 ttgctccaat tttcttatat aagttatttc ttgaatacaa aaataatacc cagtaaccct
     5401 tccaaaactc tctatattat acttatttac gtttaagtac ttattattaa attttataac
     5461 ttcatcatgt gccttactca aactaaaaat ctttttaata ttctcattct ctaattcatc
     5521 tatatttttc cctataatat tactttgaat atctagtata tcccttaaac tattattgca
     5581 aataattatg tttcccttat ctgataccat tgccatacct aagtttgata aatttattat
     5641 agaatttaat tcatctgctt ttatagtaag ttctttgtat ttagtattta ttcctgaata
     5701 taaactaatt atttcatctg tatacttaat tagcctctca gtaatttctt tatcatccag
     5761 tttgagtttt gctattattt gaataaacgt tgaactatca atatacctat ggcctaaatc
     5821 aataacttct tctatatatt ttggaactaa acttgactct cctggcgtta tagctatctt
     5881 aatatttcta tattcttcat tgggattgta tggtataaaa ttcaaatggt ttattcctat
     5941 attgtataaa ttagatattg tttccaatac agtatgctta ttatcgttta ctactaatac
     6001 atctatttct tctggcaatg aaaataactt atatatatcc ttttgcttta tgctcctatt
     6061 tatagttatt atctttgaga catcttctac atattttttt atttttaata ctctatcatc
     6121 tatcattacc aatattatgt catcttttat taattccttc tctgttattt tatttaaata
     6181 ataatttttt atcattactt tatctttaaa tatcatttct agatttttat ttaagaaaca
     6241 ttctaattta gacaacccat cagttactac tgctattgtt ttcatttgat tactcctttt
     6301 aaaatatact ctactctttt ataatataca tctgattttc actttaatag cacaaaaaat
     6361 tgtacattta gtatactaac atattccttt attagtttta tgtccctaaa tatcatagtt
     6421 gcaatatcta tcatatttct taaacttcta taaagcaaaa attgtgccaa attaataatt
     6481 ttcttaattt tattctaaag ttcctcaata gtatttacag acttagcact tatagcttat
     6541 aagtctttat attattaatc tatttttata ggttaattgt acaaatcata tatcactttt
     6601 acctattaaa tgcattataa aaaagatgga ataacgattc gtcactccac cttttttgta
     6661 ataaaaaatt gtatatataa acactattta ctactcttct aagtacaaac agtatctaca
     6721 tactataacc tttcaccatt agattctatt acttgtttat accagtcaaa actctttttc
     6781 ttaatcctag acaagtctcc acttccatca tcatacttat ccacgtatat aaacccatat
     6841 ctcttggtca tctcaccagt agatgcactt actaaatcta tacatcccca tggagtatat
     6901 ccaaataaat caacaccatc ttctacagct tccttcattt gctctatatg tgcttttaaa
     6961 tactctatac gatagtcatc atttatcact ccattagatt cttttttatc ataagctcct
     7021 aaaccatttt caactaccat cataggtatt ctatatctat cataaatttc atttagtgca
     7081 tatctaagcc cttcagggtc tatttgccat ccccattcac tagacttaag atatggattt
     7141 ttcactccac ctaatatgtt gccacttgta ttatcttctt tagaatcact acttacacat
     7201 gtagacatat aataactaaa tgtataaaaa tctacacaac cttcttttaa aatttctaaa
     7261 tctccttttt ccatttttat ttcaatatta ttttctccaa aatatctatt catataagtt
     7321 ggatattcgc cacgcacttg tacatctcca cagaaataat tcattactct actatctctt
     7381 tgagttttaa ctgcatcatc tggattacaa gtataaggat acattgttgc aaatatttgc
     7441 atacatccaa cttgataatt tgaatctatt tcatgagcta atttaacagc cttagcactt
     7501 gctacaaatt gatgatgtaa cccttgaaaa cgaagttgag gtatatctac ttggtttata
     7561 aaatctgtag tcccttcatt taatattcct tgtgataagt agcctcccat aggcattgta
     7621 gctgaattta tttcattaaa tgtcagccaa tattttactt ttcctttata tcttttaaat
     7681 attgtttcac agtaattcat atacaagtct attacctctc ttgaaaccca tccattgtat
     7741 ttctctgtta ttgcaaatgg tatttcatag tgagatattg ttattaatgg ttctatatta
     7801 tattttaaac attcatctat cacattttca taaaatttaa gtcctgcttc atttggttct
     7861 ttctccattc cagttggaaa tattctagtc catgctattg aaaatctaaa taccttaaat
     7921 cccatttctg caaataaagc tatatcttct ttgtatctgt gataaaaatc tattgcttca
     7981 tgacttggat agaatgttcc tttttctata actctcgtta ttcttttact tactttttga
     8041 gaacccccag tacacatatc tgaacaagaa ggacctttcc catctaaatt ccatgctcct
     8101 tcacattgat ttgctgctgt tgctccaccc cataaaaaat cttttctaag taccattact
     8161 ctacctccaa taatttattt atagtaaatt tattttaaaa taaaaataca tatatctaat
     8221 ttttaattaa taaaactctc ttttaagcta aaacagttat caattcctga ctattattta
     8281 catgcttttg tgtattttct ataatatcca cataatcaga ataattagta acaactactg
     8341 gtgtttctaa gcaatatcct tcttttgata tgctttctat atcaaaagtt attaatgtct
     8401 gacctttttt aatcttatcc ccttgattaa caaaagattt gaaatgttta ccttccaact
     8461 gaatagtatt taaccctata tgtatcaata gttcacaacc attatcagat ataattccta
     8521 ttgcatgttt tgttggaaat aaagtcatta tagtcccatc aaatggtgcc actacttttc
     8581 cttctttagg tgttattgct acaccttttc ccaatatacc tgttgaaaac gctgcatctt
     8641 gtatctgctc caaagccttt acttcacctt ttattgggct atatactact tctcttttgt
     8701 ttttttctac aacgccttgc ccattattat ctttagaaat ttctactata gaatcatctt
     8761 tccaaaaaaa taatgttaaa acaaatccta ctacaatagc tactattatt gctattatag
     8821 ttgcataaac acctttcata tctccagttg ttggatttat ataattaggt ataccaaata
     8881 ttccaagtcc acccattaca taacttttta cattcattat ccccattaca gctcctgcca
     8941 cagcaccacc tacacaacta tatatgaatg gtgtttttct tggtaaagtt attccatata
     9001 ttgcaggctc tattacacca catatccctg aaataactgc aggtatacac agctctttta
     9061 tcttcttatc ttttaattta aagtacattg ctgctactac tgatgtttgt gcaaaagatg
     9121 gtacaaatgc tcccactaat attgtgtcaa accccatagt cataaggttt gacatagcta
     9181 ttggtactaa tccccaatgt agaccaaata ttacaagcac ttgccacaat aaaccagtta
     9241 atacaccaca taaaattggt gaaaagttaa ataaagctaa aaatccattt ccaactatat
     9301 tagaagcata ggtagctata ggccctataa ccataaatcc caatggtaaa gaaactaata
     9361 aaactgccat tggtacaaag aaattcttta ctacatctgg tattatttta gcaaacactt
     9421 tctccaactt agctgctaat aatactatta taattactgg tactactgtt gaagtgtagt
     9481 tcattgctat tactggtaat cctaagaaag ttatatatac tggtgattca aatattgttc
     9541 cagtaaatag attgtataaa ggttgaccta tagatgctag agtatctaat tgaatacctg
     9601 aatagcataa aactgcacct ataagcattc caataaatgg ttttaatttg aatttcttag
     9661 cagctgtata tccaagaatt attggcatat actggaacat tgcatctccg attgcattta
     9721 acataatata tgcaccatca gtttctccat acaaacccaa tgctgtaaat actgcattaa
     9781 atccttttat cataccagca gcacacagaa ctcctaatat tggttgaaaa actcctgata
     9841 ttatgtctat aaacttatta agtaatcctt ttggcgcatc tgaatctgaa ttatctattg
     9901 tatctcctcc taatccagcc aaatcgcaca caacttcata aacatctgga acatgattac
     9961 caataactac ttggtattga cctgcacttt tcattacagt aactatacca tccatttttt
    10021 ttagcacatc atcatttgct attgattcat cttttaattt aaaacgtagc ctcgttatac
    10081 agtgagataa actgctaata ttatccttcc ctcctacatt ctcaatgatt tcttttgata
    10141 gttgttcata tcttctcatt ttttctcctc cattttcttt ttgaaatatg aaggtttggc
    10201 caacttaatg tcaccaccca atatatataa acctaattat taggatttat atctgactta
    10261 atacttttac ttaccactct ttcaacgtga atagttaagt ataatttttc ctcatctgtt
    10321 aaatcgtagt tgtatttttt atgtataaat tcatccaact tttctatgca tttataagca
    10381 tttttatatt taacttttat tacatttagt aaatcatctt cattattttc atgtaaatta
    10441 ttactaacaa ttcgttctgc aaaatacttt aagtgagtaa taaatctatg ataataaact
    10501 gaatcttcat caaatactat tctgtaatgg tatttaacca tatttgaaat ttcttgtatt
    10561 atttgtgtaa tctcatatat agtttttata ctttgatcca tttgtgcatt aactatatgc
    10621 agtgctataa atccagcttc atcttctgat agtctaattt caaatttttt ttctatataa
    10681 tccaatgctt ctaaccctat tttaaattca ctcttataaa atctttttat atcccaaagt
    10741 aatacatttt ttacacttac tccttcacga gctctttcta ttgcagtata catatggtct
    10801 gttaaagaaa tataaatact atcatttaat cttttcccta gtttttcttt agcatataaa
    10861 attatttcat ctgataattc catatatctt ataggaatat ctgctattag ttctttaaat
    10921 ttatcagata tatttgggtc tgaaagtttg aatatcttat caattttatc ctttgatata
    10981 tgctctcctg tacgtttttt ataagctaaa cctctcccca taactactat ttccttatga
    11041 ttttcatcag ttgtaataat tacattatta ttgagtattt tttcaataat cattgtgatg
    11101 ccccctttat tagcattaag ttaaaataaa tcttttttaa ttatttagaa tatttaaaat
    11161 gtaatttatt acataaaaaa aaacctattt attacacatt aaatagactc ccatgagtcc
    11221 tgagtaataa ataggtttag cttgtattta aacaatcact atccgtttgt attttatttt
    11281 aattattatt tattatatgt tagcaaattt attttttagt gtcaactttt tctcaatatt
    11341 ttaattctaa ataaattata taaattgcat ctgcaaagta ctatttttat aaataaacaa
    11401 aataaaaaag gtattacttg ctttaagtaa tacccattta tttttaattt aagtggtttt
    11461 tcttaatctc atcaagtagt ccatcttcta aataaatttc taatgcacat cctaccattg
    11521 cctttactgc tttgtgaagt ttatcataag cttcctcaga atttgcataa tttagaaatt
    11581 ctttttcatg aacatatacc attggactta tatctaaggc tatttctgta tacatagtag
    11641 ggcaaacttg acttacatta cctatatctg tagaacctac tggaccatct ttaccttcta
    11701 atatatctgt tatacctgac tcaatcaaat tattctttgt tatcttttga agtactttgt
    11761 tatttattat gttgtcaaaa gaattttcaa agtatctata agaaagctta gctcctgtca
    11821 ttaactctgc accttttgca cagtttataa cttttttagt cacttcatct aaataacttc
    11881 tttcagcagc tcttacaaaa aacttacaag ctgctttttc tggaactata tttggagcct
    11941 ctcctccatt tgaaactatt ccatgtattc taacatctga ttttacatgt tgtcttagtg
    12001 catttatccc agcaaaagta agttgtactg catctagtgc attaactccc atatgagggt
    12061 gactagctgc atgtgctgat accccttcaa aactaaactc taatgaatct attgccaaag
    12121 ccttacagtt tatattatta aaagcttcta tatgcatttg ataacttaca tcaacatcat
    12181 caaaagctcc tgccttaact aaatcacatt ttcctcctgt tgtttcttca gctggtgttc
    12241 ctattagtac aatttttcct ttaaaatttt cttttaactt acttaatact atacaagctc
    12301 ctagtgtaga tgctgcaatc cagttatgcc cacaagcatg agctggttct ttattgtctc
    12361 catatccagg aagtgcatca tactcagcta aaaatgctat ggtaggtcca tcattatctc
    12421 ctaattcagc tctaaaagca gtctccatat tacaatatgg ataaactgta tcaaatccat
    12481 attccttcat tttctctact aagtattttg atgatatgta ttcttcttcc cctaattctg
    12541 gattgttaaa tataaaatta caaacttctt caatttcttt tccaagttca ttctctatct
    12601 cataagctct cttctttata ctctctatca taacttacct cccactagaa tcctagatat
    12661 aatcctattg tcaaaaatac cattacaaga attgttaata ctaccattaa aggaattacg
    12721 aatctaaccc actttgacca aggaatattg gctactgcta gtgctcccat taaaacacct
    12781 gatgtaggtg ttattaagtt agttacacct ataccaaatt gataagctgt aactatcatt
    12841 tgtcttgata catctactaa gtcgccaagt ggtgcaagta caggtattgt aagtgatgca
    12901 agccctgatg ctgatggtat taaaaatgct attaatcctt ctattaagaa agttaaattt
    12961 ataaatatag tttttggtaa tccatttaat aaatttgctg cagaatttaa aattgtatct
    13021 attataaatc cattttctgc tattattacg attccacgtg caaatgctat acataaccct
    13081 gctgtaatta aatctcctgc tccacttata aatgaattaa ctatctcatc ttgtttaagc
    13141 cctgatgcta ttcctgatat tatacctata gctgtaaata tcattgcaat ttcagttatg
    13201 taccactcta gtcttaaaac tccatatatc ataatcgcca tacctattgt gaaaatggct
    13261 attaccatgg cttctctttt tgtaaattcc tttatatttg tatcactatt aaccattgat
    13321 tcttgattta gtgatatatc gtgtactaaa gattttttag ggtctttttt aactttattg
    13381 gcatacatca taacaaatcc tatagatata gccatatata ttataaacat aacaactcta
    13441 aactctattc cagaacctgg tactatttgt gataatgctt gtgctattcc tacagagaaa
    13501 ggattcgtag tagctgctcc aaatccagct gttgctccta taaatactgt ggccactgct
    13561 gtcaatgaat catatcccat actggtcata agtggaataa atatcatata aaaaggtagt
    13621 gtctcttcac tcattccaaa cgtcgtccca cctagtccaa atagaatcat tgaaatcggt
    13681 attattaaaa attgaaactt cttcatttta tttacagtat gagctataac agcttctata
    13741 gcccctgttt tattaactat cccaaaagct cctccaacta ttaaaacaaa tccaactact
    13801 tcagctgcat ctataaatcc gtttataggt gctgtaaata catcagtaat tccttgtgga
    13861 tttgatggtg ctttttcata agttccagct acaacaactg accttccgtc tatatctact
    13921 ctttcaaaat ttccacttgg cactatccaa gtaagaattg ccattaacac tattagcgaa
    13981 aatattattg taagtgtctg tggcatagta aactttttct tttttaaact gctcattttt
    14041 aatctcccca ctttttaaat taaatatttt attattgcat ccctttatcc tattttgtat
    14101 agatgtaaaa aagctcaaca tggatacatg ttgagctaaa tgtgctaaat acaacatacc
    14161 tgcatcttcc ctttatcttg tttaagatag ctctccatca aaattcggga taattttgat
    14221 gacagtactg tacctattaa atacagtccc agcactagct ttggacacta gcacttcggc
    14281 acttactcct tttatattgc ctcattggtt tccaacctct tcaatattaa tcttagtaaa
    14341 tgcaacctct acctcaatct gttcgttaga tgaggtaatt cttattaatt tttattgatt
    14401 caaagtaatt gtagcataat atgtcgagat tgtaaatatt ttcagtataa taattcttac
    14461 tttatgatgt cattccattt ttcacaaata attttttctc catataattt aaatactgta
    14521 gtatatttta aatacacaca aactttccac aaatttttaa ttattataat acctttataa
    14581 taaaaaataa aagccatgta aacttatcaa tttacacagc tttatataca ttaatctact
    14641 ccaagtacag ctttaagctt tgatgctatt gtaataactc gtggaccaaa tataacttga
    14701 actgagtttt tctttttaac aactccactt gcacccaatt catttaccca gaaaccatct
    14761 tcatagaatc catcaattga atttagctct accctaagtc tagttgcaca gtttgtaaca
    14821 ttttttatat tattacttcc tcctaaacct tctattatag aatgtacaag ctttgtatct
    14881 ttatcatcta cttcctcttc tactgcattg ttgtatgctg ttttaccagc atctacagat
    14941 tgtctttctc tatattcttt cttactataa agcttaactt cttctgaact tcttcctggt
    15001 gtttttatat caaacttctt aattgcccat gtaaatatta tataagtaac tataaaaaat
    15061 attggtatca aaaataataa tggtaacgca tccacttttt gtggttgtaa taaattagga
    15121 aacatatttc taaatgattc tcccattata gaaacattaa acatttctgt aaatacatac
    15181 gtcaatcctg ccagtggtac atatgctaca aaatacaata atggtgcaac aaataggaat
    15241 gtatactcta ttggttccgt tacccctacc aacatggctg ttactattgc tggtatcaat
    15301 atacttattg cttttttctt attagcatta tcagccaact tatacatagc taaagctgct
    15361 gctggtaata ttgcaaaatg tattaataaa cgacctgttg tgaagttacg agttatataa
    15421 gatagtgtat ctgggtctcc catttgagct aatcttatat tattaacacc ctcgtaaatt
    15481 gctccaccta cttccattgt tccacctact cttgtatatt ctatagggaa tgcaattaag
    15541 tgatgtatac caaatggtaa taatgcttta tccattgctc caaaagtaaa tgttcctaat
    15601 aatccactag aacctatcca attaccaagt ttagataatg ccaaccctac tggtggccat
    15661 ataaagtaca ttgctgtacc caatactatt gatgctataa acatcataat cattgaaaat
    15721 cttggacctg agaaaaaaga taatgcacta ggaagttcta tgtcaacaaa tttgttatgt
    15781 actgcactag ctacaaatcc agctattata gctccaaata tactacaatc atatgaaaag
    15841 attcctaaga aagaaccata taaagaactt cgactatatg cttctgcctc tcctaatcca
    15901 gatgccataa acgcttctgc tgtaactgta tctgaattta taccatttga tgctaacaat
    15961 gtacttataa ctgtgtgcat tcctataaat aaaacaattc ctcctaatgc accccatcct
    16021 ttttcctttt tagataatcc aaaagctata cctgtaacaa agaatattgg taagtatctc
    16081 attaccataa atcctaaatc gtttattact ttaaagaata catttataaa tgacccttct
    16141 tttattagtc cgctaagccc taatgcagaa acgttttcca tatttgcaaa agctgaccct
    16201 aatcctataa atactcctgc tacaacaagt aatataacag gtatcatcat tgctccagaa
    16261 agtacttgta atttatcctt catagtatta tttcctccat ttttattgaa atgccaggct
    16321 atataatgca tatatatata cttctattgc tttgtataat gtatctatac ttatattttc
    16381 atttgcctga tgtgaatttg gattatctcc ctctattgtg ggtccaaatg caactccatt
    16441 ttttaacact cttgcataag atgcagctcc ttttgtaaaa agttcggctt cttttccaaa
    16501 accttgttta tatgcacttg ataatttatt tattaattca ctatctggtt ttacatacag
    16561 taattttaaa tccttatata tatcaactat taaattttct tttttcccaa tttcattaat
    16621 tctattagtt ataaactccc aacttatacc cttaggatat ctaaagtcta ttttcatatt
    16681 caattcattt ttctctaaga agatactcat tatacatatt gtagaaactc ccatatctac
    16741 atcttcttta tatatcccta atttttttcc atgattatca tctgtaaagt atgaatttaa
    16801 aatatccctt attataacgc ctttatcatt tagcttttta attttctcta aatcttttgc
    16861 taaactaaat gcacagttat atgacctgtg tggatttctc gataaagccc tttctcctgt
    16921 atatcttact aatactttac cttcttctag ttcttctatc tctgtataat aagctaatga
    16981 ttctactaaa tctttcttat catcagtttt aaaaacagcc tcaattttgt cacatacaaa
    17041 tccatcttct ttgtttgact ttatatcaac taaattgtga tttctaaatt tatctttatc
    17101 tattttcttt cttagattga agtataaaat accattttct ccatttacta ttgggaagtt
    17161 tccatctgga gaaaatgcaa attttggctg ttcattgaca ctaaagtaat gctccataca
    17221 ttcccaagtc gtctcttcag ctccacccac tatcaatcta atctttctct tcggcttttc
    17281 attaagttct cttaagaaaa gtaatgcata taaaatccct atcaaaggtc ctttgttatc
    17341 attaactcct ctaccataaa gatagttatc tttttgacat actttaaaag gtttactctt
    17401 ccaaagttct ttattatata ttggcaccac atctatatga ttaagcaccc ctactacttc
    17461 ttctccttct ccatattcta tatgaattgc atatccatca aagtctttta caacaaaatc
    17521 tttgtctttt gctatttgaa taagcttatc gaaggcattt cttatttcta tcccaaatgg
    17581 ttgatttata tctgttgtac tttcatctct aattgaaggt atttcaacta aacttctaat
    17641 atcctttatg atattttctt gattggattt taaataatca ataattagtt ctttgtttac
    17701 tatcatctta attatttatc acccctttat attaatttta caaagcattt cagttcatat
    17761 ctacattata aattttgaat attatttttt caatatttca aatacttctc taaattacca
    17821 taatgatacg gtaaacaatc aacagattat gacaaaatca tactatacct attactaata
    17881 ttttaactta tctattacgt cttttttaat gtaattttat tccaaaaata gatataaaaa
    17941 gatgtagcct tagctacatc tttttatatc tattcaactt ccattattat gctattaata
    18001 tctacatttc caggttctat cctatctttt actgttctct taacagaatc agcatcatcc
    18061 attataacta caggagttat tatatttaaa tcatttgacg ttataaaatc taaatctact
    18121 tccattatta aatctttagc ttttacctta tctcctgcct ttaccttaac atcaaacggc
    18181 tttccttcta gtgacattgt ctctaaacct acatgtatta atatatttgt attatcatta
    18241 ctcttaatca ttattgcatg ctttgtatca aatacattga ctatttcacc atcaacagga
    18301 gaatataact taccttcatt tggtattatt gctactccat ctcccatcat cttttgggca
    18361 aaaactgcat ctggaacatc ttctaagttc actaaatttc ccttaaaagg gctatatatt
    18421 ttctcaatct tattttcttt tttcttaaat atattaaaca taacacactc tccatattat
    18481 aaaattttat gataatattt ttcttaaagc ttttgctaca ccatcattta cattagtatc
    18541 agttacaaaa tctgccatag attttacttc atctgtagca tttcccatag caataccaag
    18601 acctgcaaat ttaatcatgg atatatcatt ttcattatca cctatacaaa taacttcttt
    18661 gctatctata cctaatatat cacaaaattt tttaactgca ataccttttg aagtaccttt
    18721 actcataact tcaaaattta tctttccaga acttactact tctaaatctt cttgtttttt
    18781 aaattcttcc tttgcttctc tcaaagaatc tacgttttct gagaaaagta tacattttaa
    18841 aatttcatta tcagcttttt ctaataaagt ttcacaactt gcattttcta ttatttcaat
    18901 tctgtcttct tctttatttt tactatttat taatctatat ggatgttctt cacctatttc
    18961 attgttggat ataactattt tacagccttt aaaatgtttg tttaaattat acttttcacc
    19021 tattgtatag attttcttta tagcttcttt gctcaaaacc ttcttatact catatcccat
    19081 taatttaaaa tatgtcccat tagttgatat aatatatata tcatccccta aaacacttgt
    19141 aaaatatttc atagcattat aaggtcttcc cgtaactaag gctaccttga tacccttttc
    19201 catagcttct tttagtgctt taatattttc ttctgatact tcaaatcctt tccccatcaa
    19261 agtaccatcc atatcagtac aaattaattt atatcgcatt tgtatcacat cctagtaaga
    19321 tttttctaat atttcaacaa ggtcttcttt ctttccaaaa ggtaaaaatc ctaaaactgg
    19381 acagcttgtt atttgttcta attgttcctt tgttacatta aactcagaca tttttgtctt
    19441 aagtcccact ctctttaaaa aactttctat ttcatttcct aaagttttag ctgcttcatc
    19501 atcttccact ttttctaatt cttggtttag cattctcgct actttggcaa atttctctat
    19561 attatcctta tagaactttt ctgcaaattt tgggaacact atagccaaag cctcaccatg
    19621 aggaatatgt gtaactcctc ctattatttc acttagtgga tgtggtgctg ctgctccagc
    19681 atttgccagt gaacttcctc ctaatgtatc tgctactgct aactttgttc tgtattctat
    19741 attattttta tcttccatta ctttaggtag atagttaatg actaattcca tagatttttc
    19801 agacattatc tctgtaaagt cattagcatt tggatttata taactttcaa atgcatgagt
    19861 aaaagcatca aatgctgttg atgcagtaat tctctctggc agagttaaca taagctctgg
    19921 gtcaactata cattcttttg agaaacaatt ttgatgaaat attgtatttt tctcgcttcc
    19981 tctcgatatt actgctgctt gtgtaacttg gcttcctgtt ccagaagtag ttgatacagc
    20041 tattaatggt aattcttttt cacttacctt atcgtatatt ttaaatggat ttgtatatgt
    20101 actaaacata taatcccaat ctattttttc taaaccaaaa gtcaatgcta atacttttgc
    20161 agtatctata cttgaacctc ctccaacagc taaaacaaca tctacagatt cttttttagc
    20221 taattcgaac ccttcattta ttaattctac agttgggttt ggctctactc tatcaaagtg
    20281 tacaacttct ataccttttt ctattaaatt caatgtaact cttttatata acttgtctaa
    20341 tggctctaca tttggagtag taactaacaa acatttcttt ccatattttc ctattattct
    20401 aggtagttca cttaaagttc cttgtccaaa atttattctt gatggttgat aaaagttaat
    20461 catatttttc ctcccttaaa ttattgactt catattcaaa ttatacatat cataggattt
    20521 ttaaggcaat accttcactg ttaactctta tttactaaag cttatggcaa ttattagaca
    20581 ataaatgttt tattattacc atatcattat gtaaatttta ttaagtaccc atgttataat
    20641 tttataatca atcttattgt ataattgact taataataaa aatagagtat atacactttt
    20701 ttatgacaaa tagcttaaag gagaattaaa ttatgaaata tttgatagac agtgctaatc
    20761 ttgatgaaat tcaaaagtgt ttaaagtatg gatttaaggg aataacagct aatccatcta
    20821 tgtatcttaa agagaaggta aacttccatg aatttattga gacatgtact aatttaaatc
    20881 cagaaatatt aactgctgaa gtcattggaa ataatttata cgaattaatt aattcatgtg
    20941 ataagatatt aaatataaat aaaaacacta taataaaaat aaatttctct gaagttggct
    21001 tagaacttat aaacatatta aatagtaaag gtgtaaaaac tgcatgtacc ttgattttca
    21061 atgtaaatca agcatcatta gctataaatg caggtgctga ttacctattc ccttttatag
    21121 gtagaaatga tgaaaatggg aatgatggta taaaaaacct tacagaaata tgtaacctta
    21181 tatcaataaa tggatataat actaaagttg ttgcagcttc tattaaaaat gtattccact
    21241 taactcaagc atctctttgt ggatgtgatt atgcagctgt aacatatgat ttatttaaaa
    21301 aagcttcttt taaccagctt acagtagatg gtgccaatac ttttgaaaga gattggtcca
    21361 aggttaataa atagtaacta agatttagga gggtttgcat tgaacaaaag attattagat
    21421 attgctgaat tattactcaa cagctcagac tatattactg tagatactat tgccgaagaa
    21481 ttaaaagtta gtaataaaac tattcgtaat gaccttgtta ttttagatga atggctttta
    21541 gagtttaatt tatccctaga taaaaaaact ggttcagggg ttattattct aggtaatgaa
    21601 gatataaaac ttaaagttat tagagatatc aatgataaat caaattgtat atatgcatac
    21661 tctcctgaag atagaaaaag gtatattcta agtaaattat ttattaaaaa ttctaagttt
    21721 aggattcgtg atatttgcaa tgaactacat gttagtagag ctactgttca taaagacttg
    21781 gtagttattc aaaatttttt gcaaaacttt aaagtgactc tagttagaaa aaccaataat
    21841 ggagtatatt tagagggaaa agagaaagat attcgaaaag cagtttttga acttgctact
    21901 gttaataaaa gttatactga acttaaagaa atattattct cgaactctaa tgaatgcaaa
    21961 gataccccat caacaaaaat atttaaggag ctatttaact atgattttga gaaattatct
    22021 caaatcactc tacacacctt acaagtagaa aaaagtaacc ttagtgatga gtattatata
    22081 aacttcttaa tacatatagc tatatgtatt aagagaattt caattaaaag atatattaac
    22141 ttatctgaag gtttttttag agaattatta gaacatgaat actttgaaaa atcaaaaatt
    22201 ttatgtgcta acctaagtga agcttttgac gtagaattta ttgatgaaga ggtctgttat
    22261 atacttcttc atatagaagg attacttaaa tcaataaaga aaactactac tcttgaacct
    22321 cttaaaaatg aagaaattac agagtataat ctatctgaag caatagctgt tcattgggga
    22381 agtatactaa atttaaactt aaaggatgat ttaatattaa taaagtctct tgctaatcac
    22441 cttaaatcag ttctacacag aataaactat ggatttacta ttacaaatcc catattaaat
    22501 gatataaaga gaacatttcc ttatacttat aaggctgcta aagagtctaa tactgttatc
    22561 aaacagttga tgaattatga agtcaatgat gacgaaatag gatatcttgc tttatattta
    22621 atatcagcaa tagatagaag caaatctccc ctaaatacta ttttaatatg tcactgtagt
    22681 gatggtgtat ctaatttatt agtccaaaag ttaagttttg aatttaatca gattaatata
    22741 gttaaatcta tacctctatc ttctatttca tttacgaact ttgatgatgt ggacttaata
    22801 ctaaccactg ctcctgtaga ctttgaacat aatgctgaac tgataaatat aaatgctctt
    22861 ttaagtaaga atgatatttc cagacttagt acaattataa aaaaactata ttctgaaaaa
    22921 aataaaatat tatcttataa ataaacaaaa ataggcttgt ctcaacatag agataaac
//
LOCUS       DACTGW010000045        22601 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.045, whole genome shotgun sequence.
ACCESSION   DACTGW010000045 DACTGW010000000
VERSION     DACTGW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.045"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(197..340)
                     /gene="scfA"
                     /locus_tag="I9573_001347"
     CDS             complement(197..340)
                     /gene="scfA"
                     /locus_tag="I9573_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine peptide SCIFF"
                     /protein_id="HAT4795974.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(406..768)
                     /locus_tag="I9573_001348"
     CDS             complement(406..768)
                     /locus_tag="I9573_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HAT4795975.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(826..1119)
                     /gene="yajC"
                     /locus_tag="I9573_001349"
     CDS             complement(826..1119)
                     /gene="yajC"
                     /locus_tag="I9573_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAT4795976.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1253..2374)
                     /gene="tgt"
                     /locus_tag="I9573_001350"
     CDS             complement(1253..2374)
                     /gene="tgt"
                     /locus_tag="I9573_001350"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAT4795977.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPQDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2453..2938)
                     /locus_tag="I9573_001351"
     CDS             complement(2453..2938)
                     /locus_tag="I9573_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795978.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(2950..3975)
                     /gene="queA"
                     /locus_tag="I9573_001352"
     CDS             complement(2950..3975)
                     /gene="queA"
                     /locus_tag="I9573_001352"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAT4795979.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFLYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGNNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4293..5312)
                     /gene="ruvB"
                     /locus_tag="I9573_001353"
     CDS             complement(4293..5312)
                     /gene="ruvB"
                     /locus_tag="I9573_001353"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAT4795980.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5419..6021)
                     /gene="ruvA"
                     /locus_tag="I9573_001354"
     CDS             complement(5419..6021)
                     /gene="ruvA"
                     /locus_tag="I9573_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAT4795981.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASSAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(6014..6523)
                     /gene="ruvC"
                     /locus_tag="I9573_001355"
     CDS             complement(6014..6523)
                     /gene="ruvC"
                     /locus_tag="I9573_001355"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAT4795982.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6835..7464)
                     /locus_tag="I9573_001356"
     CDS             complement(6835..7464)
                     /locus_tag="I9573_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795983.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKNNGVQTSK
                     VVSASANMDEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMNATIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7667..7990
                     /locus_tag="I9573_001357"
     CDS             7667..7990
                     /locus_tag="I9573_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HAT4795984.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8258..9112
                     /locus_tag="I9573_001358"
     CDS             8258..9112
                     /locus_tag="I9573_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795985.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9298..10734)
                     /locus_tag="I9573_001359"
     CDS             complement(9298..10734)
                     /locus_tag="I9573_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAT4795986.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTHGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKEMLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10805..12187)
                     /locus_tag="I9573_001360"
     CDS             complement(10805..12187)
                     /locus_tag="I9573_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HAT4795987.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAVLIGGWIYGKRFEKYPAYAIDENDREKN
                     LEKGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKRYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(12323..13228)
                     /gene="garR"
                     /locus_tag="I9573_001361"
     CDS             complement(12323..13228)
                     /gene="garR"
                     /locus_tag="I9573_001361"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAT4795988.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            13624..14289
                     /locus_tag="I9573_001362"
     CDS             13624..14289
                     /locus_tag="I9573_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4795989.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDVYKTTLIHMETPKYINLEDFC
                     NNK"
     gene            14406..14945
                     /locus_tag="I9573_001363"
     CDS             14406..14945
                     /locus_tag="I9573_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795990.1"
                     /translation="MNENVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEEFGE
                     DKTIEILRKVLFERSEERAIAMRERALENGDELIADNIISTTDVPFLGWVPEFKELHC
                     PYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCERIY
                     FHDDKVSEGKYTYGNKNCK"
     gene            15207..15515
                     /locus_tag="I9573_001364"
     CDS             15207..15515
                     /locus_tag="I9573_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795991.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(15677..17467)
                     /locus_tag="I9573_001365"
     CDS             complement(15677..17467)
                     /locus_tag="I9573_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4795992.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(17467..19194)
                     /locus_tag="I9573_001366"
     CDS             complement(17467..19194)
                     /locus_tag="I9573_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4795993.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSARVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(19439..20003)
                     /locus_tag="I9573_001367"
                     /pseudo
     CDS             complement(19439..20003)
                     /locus_tag="I9573_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
     gene            20292..21404
                     /locus_tag="I9573_001368"
     CDS             20292..21404
                     /locus_tag="I9573_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid racemase"
                     /protein_id="HAT4795994.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     AEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(21587..22414)
                     /locus_tag="I9573_001369"
     CDS             complement(21587..22414)
                     /locus_tag="I9573_001369"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAT4795995.1"
                     /translation="MSRKYSISSMCKLIGVSRSGYYKWLSYSKKSSDRGIKDRIIKDY
                     IIEIHKKYRGTYGRKRICTYLNKILDSPINHKKVYRLMKELGIKSIIRKKVYRRKFKS
                     YEVYDNILNREFRANQPLEKICMDITYIPIGKKFLYMNVAKDLFNGEIVAYEISTKMD
                     TKLVNKTVNQLINMNLAKDCILHTDQGSQYTSRSYSKRLKDNGIIQSMSRRGNCWDNA
                     PIESFFSHFKSELIYLIDTTDPKEMISLINDYIYFYNNERIQLKNGMSPIEYRTHSA"
     gene            complement(22441..>22601)
                     /locus_tag="I9573_001370"
     CDS             complement(22441..>22601)
                     /locus_tag="I9573_001370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795996.1"
                     /translation="KTAFNEETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKL
                     STLLDKSK"
BASE COUNT         7518 a         3837 c         2670 g         8576 t
ORIGIN      
        1 agagtagcta ctttaaaata aatgctctta tatgattaga tatttttgca taattaaacg
       61 ataagaaatc aaatttaatt ttacataagt agaatatata tttttatata ataattgaaa
      121 ataaaagagg atacctcata atgataaaag aatcactttg agataccctc ttacaaacaa
      181 ctaaatcaat aattttctat ctttcacact cttgatttgc aactgtacaa gaagttttac
      241 aagctgattg acaagaagtt tgacactctc cacatccacc ttttgcagca ctttgcttta
      301 aagtagcttg agataaagtt tttacatgtt tattttccat tttcaacgcc tccatttcta
      361 attgtttcct attgattata tcataaaact actttaatat aaactctatt taaagttgac
      421 acccaatacg cctccaatgc ctcctgctgc tgatattagc aataacttat aaatcatccc
      481 tacttcaaat acaaactttt cttgtgctaa aaacacagtc aaaaaaatac atactatgta
      541 caaaagtcct actaaaagtc catacataag tcctttttct ttcatgtgtt ttgaagtgta
      601 aaaccctcct atagctgcag aaatagtagt tataaatgat gagaccatag ttatattatc
      661 tccaccaata tctgtaaatg tcaaaaataa gttatacact aataaaacag ccaaagttat
      721 tatatatgca tatcctagac ctttaaaaat ataattagat tttcccataa aaaaacctcc
      781 tctcaaatta atatatattc gagaaaaggc ttttaaatta ctaatttagt cttcttctat
      841 ttcttctttt ttagatttaa cagttcctat agcccatttt tcaaatggaa ctttagttct
      901 atttggtcct aattctaata ttacaacatc atcttcaact ttagctacat tagctactat
      961 tccacctatt gtaatgacct tgtctcctac acttaggcta cttctcattt cttttaattg
     1021 tttgtctttt ttcttttgag gtctaatcat taggaaataa aagattgcga ttactactac
     1081 ccatagacct atactcataa ttatttgttg ctgtggcatt aatttttcac tcctttgtaa
     1141 attatactct tttgtaaatc ataataaatg tttatataac aatttacttt ctctatcttt
     1201 gagtactatt gaatattata tattaaaatg attataatta atagtgcaaa acctatattt
     1261 catatccata ttttgcaaaa aattcatttc taaaatcaag taatctatct tccattatcg
     1321 cttgtcttat ttgtttcatt aaatttaata aaaagtgaag attatgagta gttattaatc
     1381 ttgcacccag tatttcattt gcttttataa ggtgtcttat ataagctctt gaatagtttt
     1441 tacaagcata acaatcacat tcagggtcaa gtggagtaaa gtcttcagca taagttgcat
     1501 ttctaactac tactttacct tggctagtca ttgcagttcc atttctagcg atacgagtag
     1561 gtaggacaca atcaaacatg tctactcctc ttataacacc ttccactaag tcatctggac
     1621 taccaactcc cattaggtat cttggtttat cctgtggcat aagtggagtt gtatgctcta
     1681 gcacgtcata cataagtggt tttggttctc caacactaag cccaccaata gcatatcctg
     1741 gtaaatcaat actagttatt tcttttgctg attgttctct taagtcttta tacatcccac
     1801 cttgtattat accaaataaa gcttgtttgt cggtattatt atgggcatct ttacatcttt
     1861 ttaaccatct tgttgttctt tctaaagaat tttttacata ttctctatct gatgggtatg
     1921 gtgcacactc atcaaacgcc atcattatgt ctgaacccaa tgcattttgt atttccatag
     1981 ctttttctgg agttagaaaa tgttttgaac catctaagtg tgacctaaat tctacacctt
     2041 cttcttttat ctttcttaaa ggccctaagc taaatacttg gaaccctcca ctatctgtaa
     2101 gtattggttt atcccagttc ataaacttat gaagaccacc agctcttttt ataagttcat
     2161 gacctggtct catatataaa tggtatgtgt tactaagtat tatttgtgaa ccaatctctt
     2221 ttaattcttc tggtgtcata gattttacag ttgcttgagt tccaactggc atgaatattg
     2281 gtgtttcaat aatcccatgt ggagtgtgta atcttcctaa ccttgcacca ctctgcttac
     2341 aagtctttat caattcatat cttactgcgt acataaagtt ctccttaacc atatttattt
     2401 ctcattattt cttaagatat atttgtaata agctattcat ataatttatt gtctaaatag
     2461 atttatcttc gtcttctttg ttctgttgct ccttaattgc ttcttcatca tgaatactca
     2521 ttatattaag agcactcttg tcttttgtta ctaaaaactt ttttatagca aatagtcctg
     2581 ctatagctat aactcctatt aaaacatcta tcataccttc atttttaggc agtagcaaca
     2641 tttttctagc tattgcgtaa agtaatgcct caataaatgc tcctggtata tgaagagata
     2701 gcattactac aagttcaaca cctattacta aaagtaatat ttgtgctaaa aaatcattaa
     2761 gctgactgta ttgtactgga ttttgaaaat caactatata tgctccccac ataagtctta
     2821 gaacatctat agttcctaaa aatactgcta ttagaactac tactgctaaa acacgttcaa
     2881 atataaaagc aatctttaat atagggttat tctttttttg tttttgtaaa ctatgcattc
     2941 attacctccc tactttataa tcatagcatc tccaaaactg aagaatctat atctttcttt
     3001 tactgcctca ttatatgcat ttaaaacatt atcctttcca caaatagcac ttactaacat
     3061 tattagtgta gattctggta aatgaaaatt agtaataagt ttatctaaaa ctttaaattt
     3121 ataacctgga tatataaata tttctgtcca tccactagtt tctttcatct ttccatcctc
     3181 attacaagct gattctatag ttctacaact agtagtacca acacatatta cattgtttcc
     3241 attttccttc gctctattta ttttgtcagc tgcttcttgg ctaaccatat aatattctga
     3301 atgcatcttg tgatttaaaa catcttctac ttttactggt ctaaaagtgc ctaaaccaac
     3361 gtgtagagtt acaaaagcta tatctacacc tttttctttt attttattta acaattcttc
     3421 tgtaaaatgt aatcctgctg taggtgctgc tgctgaacca ttatgctttg aataaacagt
     3481 ctggtatctt tctttttcat ctagtctagc agtgatatat ggaggtagtg gcatatttcc
     3541 aagttcatcc aatatttctt caaaaattcc atcataaagg aattttataa ttcttgaacc
     3601 ttcatctgat aaatctacaa cttctccaat tagctttcca tttccaaaag aaaatttagt
     3661 ccctactttt gctctttttc ctggttttac taaagcctgc caagtatctt cctctgttct
     3721 ttttaataat aaaaactcta ttttacctcc agtttcaagc ttctctccta ttaatctagc
     3781 tggtataact ctagtattat ttaatactaa acaatctcct ggatttaaat attctattat
     3841 atctctaaat actttatgtt caatattacc tgtctcttta tctaatacca tcaatcttga
     3901 gctagctcta tcttctatag gaacttgagc tataagttcc tgtggtaagt caaatttaaa
     3961 atcacttgtt ttcaatgtta aacttcctct cttaaaattt ctccagctac aataatatca
     4021 aaaaaacaac ttattttcca caatattacc ataatctatc aagaaattgc tatttttaac
     4081 agacaccata tccacaatat atagatattt ttccacgata tacatgtctt atccacaata
     4141 tatagataca atacacacaa tttttatcgt gtttatccac agtatctaca gatataatta
     4201 ccattttgtg gatatatttg aatttttagc cattttagaa atattttaat ttaatgactc
     4261 ttttatttta taaatactcg tataatctat ttttattttt cattaggata tggaattttt
     4321 aagtgttcat atgctaatgg catggctact cttcctctag gacctctatt tataaatcct
     4381 aattgaagca aatatggctc atatacatct tctattgtat ttctatcttc tccaatagaa
     4441 gctgccaatg tatctaaacc cactggtcct cctctaaatt tttctattat agtcataaga
     4501 agcttctcat ctacaaaatc gagacccaaa ctatcgactc ctaaaagttc cagagcatct
     4561 tttgccacct tatcagttat ttttccatca gctctgactt gtgcaaaatc tctaactctt
     4621 tttagtaatc tatttgctat ccttggagtc cctcttgaac gttttgcaag ttctaatgct
     4681 ccattagact gtatctctgc atctaagatg cttgaagacc taagtactat ttttgaaagt
     4741 tcatcaactg tataataatc caatttacaa ataacaccaa atctatctct aagtggattt
     4801 gtaagcatac cagctcttgt agtagctcca ataagtgtaa atttaggaag gtcaagtctt
     4861 atgcttctag cagatggccc tttccctatt attatatcta ggcaaaaatc ctccattgct
     4921 ggatataaaa cttcttcaac acttctattg attctatgaa tctcatctat aaataaaaca
     4981 tcattttcat ttaaattcgt aagtatggct gccaaatctc cagctctttc tattgcgggt
     5041 cctgacgtaa ttcttaaatt tacccccatc tcatttgcta ttatacttgc tagtgtagtt
     5101 tttccaagac ctggtggtcc atataaaagc acatggtcta actgctcatt tctcgattta
     5161 gctgcttcta taaatatact aagttgttct ttggattttt cttgacccaa ataatcttct
     5221 aaagtttttg gtcttaaact gttttctata tctatatctt ccatcttcat tgtagaagtt
     5281 attatcctat tttcatcttc aaaaccttgc atttataccc ctcctctcgt acttttgaaa
     5341 atctacttta tatattttca aatttacata aactccttaa atattgtttt aaaatccaaa
     5401 tattataaaa tccaaatact atttcaagga tttactcatt atatatgtaa gagctttctt
     5461 tataatagct tctgtattca taccatcttt tttacatttt tgaactgcct cttttgattc
     5521 cgacaaagta tagcctaaag ctacaagtgc atctacagac tcatcttgtg atatcaaggt
     5581 aggtttttgt gataataacg ttggttcaaa ctctatgtta tttttgtcta ccttatcctt
     5641 aagctctaag acgattcttt ctgctgtctt tttaccaaca ccaggagctt tggataattt
     5701 tccaatgtct tcacttaaaa tatatgctcc taactgtgct gatgatgcaa atgatagtat
     5761 tgaaagcgcc accttaggtc ctattttaga tacagatgtt agtaattcaa acatcttaag
     5821 ttcttcccta cttacaaaac cacaaagact catatcatct tctcttacaa ttaatttagt
     5881 atatattttt gtggcttctc ccacttgtaa attcattatt gtattggatg atacatttat
     5941 tttatatcct atcccattat tttcaacaac tatactgtct atataaattt cttctactgt
     6001 accttttata taactataca tacttaccac ctatattctt tagagttttt tcaagtttgt
     6061 ttgaatgagc atggcaaata gccacagcta aagcatctgc aacatcatct ggttttggaa
     6121 ctttttttag acttaaaaat gaagttacca tctgttgaac ttgtgcctta tctgctctcc
     6181 catatccaac tacaccttgt tttacttgaa gtggagtgta ttcatataca ggcttcccat
     6241 tatgagcaca tgcaagcata gtaacacctc tagcttgtgc aactgttata gctgttttta
     6301 cattcttgtt aaagaataat tcttccattc caacttcatc tatattgtaa ttctttacta
     6361 ttgtatcaat tcctttatac acaagttcca atcttctcga tatattcata tgggcaggtg
     6421 ttgtaactgc tccataatcg attactttaa acttgctatt tttgtattca attataccat
     6481 atccaactat ggctatacct gggtcaatcc ccaatattat cattttttaa aattccccct
     6541 aaaaataaaa gaaataccaa aatgtatttc tcataaaaac ttatgttcta aatcattata
     6601 tcataaataa tattaacaaa tcataaaatg ctctatgtaa aatataaaat caaataaaaa
     6661 agttatatca aagcaagatt ttacctcaat ataactttta tatttattac aaataaatta
     6721 ttgtattttt ataggaatta aatgtgacta caattttaat actaatctat ttaatattaa
     6781 attatttatt attaacttac ttagtattta acttgaaata atattaattt actattaact
     6841 tgcaaagtct tctaatcttg aataggcctc ttcttcagta tccataacta cacctttttt
     6901 gatttcatct tgaactgttt taggaagtga atctactctt atttttgttt tttctactaa
     6961 tgttttactt ccactatcat catatttata tagtgctata tttccattat cattttcaat
     7021 agtatattta ccttttatag tagcgttcat tgcttgtaca tctttattat cactatattt
     7081 agattctacc aatatgattt catttttatc catactcttt atttttttag ttggatattc
     7141 ttttttaaaa tatgatacta tttctgattt tgtcttatct aataatgact ctggaactat
     7201 cccagccatt gtaagtgttc tcttatagtt agtttcattt tcgccttcac tttgaagcca
     7261 aacttcacaa tttttattta atccaaaagc ttcttttgac ttttcgtcca tattcgcact
     7321 agcacttaca actttactag tttgaactcc attatttttt aatcttgtcc ctatataaac
     7381 tccgctactt aatacaaata ctccaaccag tattaaggca gatattttcc aaggaaataa
     7441 ttcttttttc ttcttttcaa acattgatta cgcctccttt ttctattatt ttctccaagc
     7501 tgtaagtttt tatacaattt aggtaaattt tagaagaaaa tatatttata tttcaaatta
     7561 ctctaagaat agatattttc taaaaggtag ttttctctta atggttatat tcttgtaatt
     7621 atatggacat aatttataat aaataatcgt ttaggaggta ataactttgg aaagaataaa
     7681 ctgtaatgta attaactgtt cacataacaa cagccatata tgttatgcag ataaaatttt
     7741 tataaatggt gaaaaagctc acaatgacag acatacttgt tgtagttcat ttttagatga
     7801 atctatttat agtaatttat ctagcaacgt taataataga aattgtgagg aattagtttg
     7861 tactgtaaaa aattgtaaat acaacaaaga tggtagctta tgtacacttg ataatatatt
     7921 ggttgctcct gaaactgata gagtaaacct ttatattgaa acatgttgtt ctagtttcga
     7981 atgtaaatag tcatagtata aatataaggg tgtcaaatgt aattaaacta acatagtgta
     8041 gagtatctac aactattagt ttgattatac ttgacaccct tatttatttt aaataaaatt
     8101 tcattattat aaccatgttt ataatgttct aactgtaatt aaatttgata aatttctata
     8161 aattggtata atcaatatgt aaattgaatt tagcaaatta atcataaaaa taaaatatcg
     8221 agtatatagt gttatactaa ctagaaatga ggtgcttatg agtacatttt atattcaatt
     8281 tctttatttt gtaatttata gctttatagg atggtgttgt gaaactacat attgttcagt
     8341 tttacaaaaa caatttgtaa atagaggttt tttaaatggt ccattttgcc ctatatatgg
     8401 atttggagca ttagcaatag ttgctacctt gactcctttt gtacacaata ttcctttgtt
     8461 atttttgttt agtataatta taactagcat aatggagtat tgtacgagct ttattttaga
     8521 aaaaatattt aatatgactt ggtgggatta ttctaaacat aaatttaata ttcatggaag
     8581 agtttgtctg gaaaactcac ttatgtttgg tgtattatca ttgattgtaa tgcttatagt
     8641 acatcctatt gtggttgact ttattaactc tatctctaaa aatgtgctat ttatatttgc
     8701 tatatctata gaaatttatt ttgttttaga tttagttatc acagtccata caattttaca
     8761 attaaatggt aagttaaaac aaatcaatct tataattaag gaattaaaag ataagaaaga
     8821 atactacaaa cttattacac aagaaactat agaaaacaaa cttgaatctt tagttgaaaa
     8881 tagactggat actttggaag atagatttga aatacttaag gaaaaagaaa actatcgcta
     8941 tgctaaaaat cgtattgatg agctaaaaaa taaacttaat actttgctgt ctaaccacaa
     9001 attacttcat agacgtataa tcaaagcatt ccccaacata agctctaata agcatacaga
     9061 tattttaaat aaaatcaaag aaaatattaa atcaattaaa aaagataaat aataaaataa
     9121 acacataagt taaataataa aaatactaat acataagatt aaggtttaga atattttttg
     9181 taaaatactc taaaccctaa ttaatatact attattttta atcttaattt ataattttag
     9241 tttattttac ttttaacaca gtaaatttaa gcgtttaaac ttcaatacac taatatacta
     9301 ccccatcaat atctcaactg attctctaag tgtgctaatc tctcccacat ttccagggaa
     9361 aattatatat ggcatattag gaaatttgct ttctttgcct gtcatccata ctggtatacc
     9421 aggttttatc tgtcccatta ctgtagcttt ttttactctt agtgctttag taccaacatc
     9481 actagaagta attccaccct tagctataat aaaatttggt cttacattaa gcataccaat
     9541 tatactggta actgcatctg atatttctac agaaatcata agctgtttat ctttatcatt
     9601 cgtatccaag tcaaaacgct ctcgtctagt ataaacagca acagttttac cactgcttat
     9661 tttttcctct acaactttta taactctttt tacctcattt tttagaccat tttcctgaag
     9721 aactaaatgt tgattaaatt ctataaattc tattggatat ttacaatttt taagttcttc
     9781 taactgttgg gtagtcttat tcacatgtga accaacaagt ataattcctc cattggtatt
     9841 accttttgat actaactcat ccttagtaag catttcctta tcactcactc cgccaagtac
     9901 ttttgttatg gcagcagcgc ttctaaacat aaattcctta ccactattta ctgctcgtat
     9961 aaacgcaatt gtaaatacct ttacatctac ataatcggtt gcatttacaa taattttatt
    10021 gaatccttca gcttttttaa gtttttctgt tatagcttca atatttagct ctctaagttc
    10081 tttagaagaa atatatatca tatcactaga tttgtacttt ccatgtgtct tttcttcaca
    10141 ccactcacct atatcagatg atttatatcc aaaagattta tcctttgcaa attcagtcat
    10201 tcctgctggt acaagtattt ctccttcttt aacataatgt acattatcta tagtaaacct
    10261 accaccttct ttaaaaaatg gcattattat ttcaccctga aataacttcc ccgataatct
    10321 ctctacttcg ctttttaata aattagtttc aacaggataa tgtcctctca tagtagaatc
    10381 actacgactt atgattataa aatccttatt aactttcttt gatacatcca caatattctt
    10441 tgaaatttct ttatgtgctt ttgtagtttg agcaacagta aatccccttg aattagtaag
    10501 tataaaaaac atactatttt tctcattaaa accttgttcc atactatcct tatcccaatc
    10561 agtataaaca gaaatatcat gaactgtttg tacacctgtt gggtcatcat ctaatacaac
    10621 aatttttcta tctaaatgct taagctcatc tgcaagaagt tcatttaatc tgtcctcatc
    10681 tacttgttta aaagtattaa gtatatctat gctaattggt tttacttctc tcatttaaaa
    10741 cgccccttta tgtaatttgt atagtctaaa tgtaatttat ataatataaa caatagtttg
    10801 ttatttatag acctggaagt ggtataattc catttataac actaagcaat aaaaccatta
    10861 taaatgctac aactccagca atggttcctc caattgtcca agagtttaaa gtatctttaa
    10921 ctgataatcc actaaatctt gaaagtaccc agaatccaga atcatttgga agggagaaac
    10981 caactcctcc agcacatata gcaagcccta ctaaaactgg tgacattcca gttgccaaaa
    11041 ttgtaggtcc taaaattgat gatgttgtta ctaaggctac agtagcagaa ccttgagaaa
    11101 ttctaagcaa agcacttaat aagaatccaa gcactacaac tgatatacta agaccagaca
    11161 tagtatcaac taagaagtta cctataccac ttgcatttat aacactgcca aacgcaccac
    11221 ctgaacctgt tatcaataat attagccctg ctgaatcagc agcttcaata actacatctc
    11281 ccataggttt atttatgtat ctctttaaaa atcctattgc caccaatatt ccaagaagta
    11341 ttgctatgtt tttatctcct ataaatgcaa gtactccact cattgtagaa cctttttcta
    11401 agaacatact aaatacattt gatataagta taagaagtat tgggaacaat aaagaaaaca
    11461 tagaaattcc aaaacctggt actttagaag aatcatcatc ttcaattttt atgctatctt
    11521 ttcccttttc taaatttttt tctctatcat tctcatcaat agcataagct ggatattttt
    11581 caaaacgttt tccataaatc catccaccta taagaactgc tggaaaagca acaattatag
    11641 aataaagtat aaaactacct acatttgcat ttacatttgc tgcaactgct aaaggtcctg
    11701 gtgtaggtat tacaagtgca tgacctacta tagtcccaac tcctaaggca catacaaaaa
    11761 ccattagaga tttcttagtt actctcgaaa catacttaat tataggcatc attataacaa
    11821 aagctgcatc cataaaaact ggaattgaaa ttaaaaaacc tgctgtagtt actgctaaag
    11881 cactgttttt ttctccagtc ttagctaaca acccctttgc aatagattct gttgctcttg
    11941 attctgaaag aatatttcca aatattattc caagtccaat tacaatacct atactaccta
    12001 atgtatttcc aaatccagtc gatattgtgg ttgatatttc atttataggc attctaacca
    12061 taaagcctgt aattatagat gttatcagta atgccaaaaa cggatttact ttaaatttaa
    12121 taatcgatac aagtaaaatt cctatagata taacaaatgc tatcaaaagt ccatatcctg
    12181 taatcataat ctttacccct ttatcattct actttattat ttatcaattt tatttttctt
    12241 ttactatgat aacttaacct cgctgtattt atataatcta atcctaataa tatgtattta
    12301 attatattga tatatttaaa tattaattat tgtgtttttt tatttcaaca cctgccaact
    12361 tttcaaaata ctgtgctatt ccactatggt catcttccaa atgtccatgt acttttaatg
    12421 tctgcataat ttctaataat tgacttgtca taggtagcgg tacatctaaa ttgtgtgctg
    12481 tttgcattac attcttaata tctttcaaat taatagatat ttttccacct ggtttaaaat
    12541 cacgattcat aatcattggt atcttcgcat ccaatattgt actaccagct aggcctcctc
    12601 ttattgcctt atatactttt tctgggtctg cacctgcttt tgctgcaagt acaaatgcct
    12661 cagaaactgc tgctatcgtc aaattaacta ttacttggtt tgtaagtttt gtaacacttc
    12721 cactaccatt atctccaatt aaaatagatg agcttcccat tatattaaaa aatggctcta
    12781 ctttcttata aatttcttcc ttaccaccag ccataaaagc aagtgtacca tcaatcgctt
    12841 ttggctcccc tccacttact ggtgagtcta taaattctaa gcccatatct tttaatttat
    12901 ttgcacacaa tattgattct gtaggagtaa ctgaactcat atcgactact atacttcctt
    12961 cttttaaagt ttttgccaat ccatccatcc caaataatat atcctgaaca atagttccat
    13021 ttggaaggat tgtaaataca atatcacatt cttgacccat ttctaaaaca cttgcgtatt
    13081 ttgctccaca tgatataagt tcatccacag cagatttatt aatgtcatac accaaaagat
    13141 tacatccatc ttttaataaa ttttttgcca ttggctttcc cattattcct aatccaataa
    13201 atcccaattt cattttataa ccctccatat cttcacattt ttgaaaacgt tttttatgtt
    13261 ttagagtata ctacatacca gtataccggt caatataaaa tataaaagta tttattttaa
    13321 caataaatag taatttttaa agtcatttat tatatttagt catcctatat aagttttaat
    13381 acatactatt gtttttaaat ataatatgct acttttacag ttttgtattt ttactaattt
    13441 caattagttt taattaatct gcctttaatg ttaatcatac ttcacacttc aataattttt
    13501 tatatttttt aatcttatct gcaatttaaa ttgcaaaaga ttgaccttat tttcactatg
    13561 ttgtatgctt aaaattatat aagtttttgt atttttatat tgtttagggg ggtattatta
    13621 agtatggcta catttaaaag attacaagaa caagcatatg actacttaaa agaattgata
    13681 ctatctggag aaatgataga aaatgaaata tattctgaaa caaaacttgc tagtgaaata
    13741 ggtatctcta gaacacctat aagagatgct cttcaaagac ttagtcaaga tggatttata
    13801 gatataatcc caagcaaagg atttagaatc catcaaatta cagcaaatga aattgtagag
    13861 atattccaaa tacgctctgc aatagaaggg ttttgtactt ttttaattac aagtcaatac
    13921 aaagaagcta gggcagtaga aactataagt aaattaaaac atcttttaga taaacaaaaa
    13981 gatattttac taggtgataa aaatttaaac tcatttgcag aatacgatac attatttcac
    14041 acaactatag tttcatatgc tcaaaatact gaatttgaca aaatgtttaa caactatatg
    14101 tacagaataa aaaaattagc tctcgattca ttatcacatg aaggaaggct tgaaacaaca
    14161 ttaaaagaac acacagatat ttttaataat attgcaaatg gatgtattga agatgtttac
    14221 aaaactactc tcattcatat ggaaactcct aaatacatta atttagaaga tttttgtaac
    14281 aataagtgat tttagtatta tactttcatt tattaaaaat atatctacct caaattaatt
    14341 tcctaattta taaaaaatat tctataatta tattgtaact aatactattc taaggagtga
    14401 tttttttgaa cgaaaatgtt gtaactaatg aaactattga caaagattat gctattgaag
    14461 aagttcgtat ggcatgcaaa cattttggtg acttatattt ttatttttcc aaagtgttat
    14521 ttgaagagtt tggtgaagat aaaactatcg aaatactaag aaaagtttta tttgaaaggt
    14581 ctgaagaaag agctattgct atgagagaaa gagctcttga aaatggagat gaattaattg
    14641 cagacaatat tattagcaca actgatgttc catttctagg atgggttcct gaatttaaag
    14701 agttacattg cccttatgga gcttcctggc ttttaagatt tgaggaaaac ccttggttta
    14761 aaaaattcgc ttctctttac tgtgatgtta cagatactac tgtagctgaa gtatttacag
    14821 gagatacgtc ccataaaata acaaaaaaca ttctatgggg agataaaagt tgtgaaagaa
    14881 tatatttcca tgatgacaag gtatctgaag gtaaatatac ttatggaaat aaaaactgta
    14941 aataaataat ttaaattttc tatattcata tactatattt tgtacttttt catataaata
    15001 ttataactag tcttttattt tacttttgtt taacctaaag ataatttact tttaatctat
    15061 atgaattatc atataaataa tcagaaagtt atctttataa attatagttt tagatttata
    15121 atttataaaa tatttatgtt ctttgtaaaa ttttttagaa gcttctaatg ttatgctata
    15181 aaactatctt gagaagggct gattgaatgt ttaataaaat atatgaaaag aaagaattta
    15241 tagtttttca agttaaaaaa ggttatgtag tttataatac tagaaagagt tttgaagaag
    15301 gtcatacaca tttaaagcat tttgaagcag caaagacagc tattgattta gcaataaata
    15361 aaaagattcc tagaagcaaa gatggatatt atctgactag tttaattcgc ataagtgatg
    15421 atggttatta cattgataag ttaagcgaat tgttatatgt tagagaacaa aaaggtaaaa
    15481 aagaaaagta ctgtaattct ggttatcaga tgtaacaaga ctagttgtga attataagta
    15541 ccaatcataa atacctttag tcttaaaatc aaagagtatc agtaaatcta taaaaaaaca
    15601 acttactgat actcttttta attatatgat taatatttga atttgataca aaattttata
    15661 caaaagttta tgttatctat tctgattctt tgtctgcaaa ttggctatta taaagcttct
    15721 cataaaatcc acccttttcc attaattcat catgattccc ctgctctata atacttccat
    15781 tattcataac taaaatcaag tcagcattgc gaattgtaga aagtctatgt gctataacaa
    15841 aactagttct accattcata agatttctca ttgctttttg aagcataagt tccagcctag
    15901 tatcaacaga actagtagct tcatcaagta tcaatattga agggtctgat ataaaagctc
    15961 tagctatggt taaaagctgt ttttccccta gagatatatt agatgcttct tcatttaaga
    16021 acatattgta tccatctgga agagtggtta taaagtgatg aacatttgct acttttgctg
    16081 cttgtataat ttcttcttta gtagctccaa atctaccata ttctatattt tcaaaaatgg
    16141 ttccattaaa taaccatgta tcttgaagca ccataccaaa cattgaacgc aaatctttac
    16201 gtttcatatc tcttatatct actccatcaa ttttaatagc acctttcttt acatcataaa
    16261 acctcatcaa taagtttatt aatgttgttt tacctgctcc agtaggtcca acaatagcta
    16321 ccatctgacc actttttact tctgcattta aatcttctat taaagttgaa ttttctccat
    16381 atccaaaatt aacatgctca aatgtaacat ttccttttac attttctatc ttaactggat
    16441 tttcaacatc aggtatttcc tcttcttcgt ctaaaatttc aaatacacgt tctatagctg
    16501 caaaagaaga ttgtattact gtagataact gagtcatttg tgaaagtggc tgatttatct
    16561 gccatatata acgcacaaat gcttgtaaat ttcctactgt tatccctcca cttactgcat
    16621 atatagaacc gacaactgcc acagctgcaa ttccaaagta agttactaat gaaaccatag
    16681 gattcatcat acttgatata aattgtgcct taaatccatt ttcacaaagt tcttgattta
    16741 cttttttaaa ttcttttata gaatcttctt ctttaccata tagttttatt tcattaaatc
    16801 cagtatacat ttcttgtact tttccattta attttccaag agcattttgt tgcaatgaaa
    16861 atctactttg agattttttt actacaaatt tagatacaaa ataactaatt ggaattataa
    16921 aaagagctat aagagccata tatatattaa ttgtaaacat cattgaaagt gccaaaatta
    16981 tgactaagat tgagtttaca atttgcataa aactctgttg caatgcattt gatattgtat
    17041 ctacatcatt tgatatacga cttaatacat ctccaaaact attactatca aaataactaa
    17101 taggcaatct tcttatttta ttttcgactt catttcttaa atctctcatt gtgttttgaa
    17161 tagcattcgt aagtagaaaa cttgaagtat acgtaaaaat tgcattagca aaatatatac
    17221 atgctaaaat caccaatatt tttgttacat aatcaaaatt tacggaagcc cctggtactc
    17281 cttttgcgat actaattaca tctttagtca attgtgttat tatcaatcct tcagttttag
    17341 gtgcaagtgc attcatagta gctgctgaaa ttatacatat aatagctata ataaaggaaa
    17401 atttatattt ctttacaaat ggaagaagtc taccaattgt tttcttaaaa cctttagact
    17461 ttttcattat tctaattcct ccttttttaa ctgagatgtt gcaatttcat agtatatttc
    17521 acagtttttt aataattctt tatgagttcc cattccgaca acttgtcctt cattgagtac
    17581 tataatctta tcagcatcaa taatagaact aattctctgg gcaacaatca atacaattgc
    17641 atcttttgtt tccttcttca atttagcacg aagtattgca tccgttttaa agtcaagtgc
    17701 agaaaaacta tcatcaaaaa tgtatatttc aggtcttctt ataatagccc ttgcaataga
    17761 aagtctttgt ttttgacctc ctgatacatt tgctccacct tcactaatca attcatcaaa
    17821 ctttctaggt ttttttgata taaactcata tgcttgtgca actcttgcgg aatattctaa
    17881 ttctgactct cctgccttat gtttcccaaa tctaatatta tttgctatgc taccagtaaa
    17941 aagcagtgat ttttgaggta tgaatcctat cttttttctt aaagctttta aatcatattc
    18001 tcttatatct actccattta tcttaatgct accctctgtt acatcgtaaa atctaggtat
    18061 caagttaatt agagtacttt tcccactacc agtacttcct ataaaagcga ctgtttcacc
    18121 tgtttttgca gtaaatgaaa tatccttaag tacagaagct tctccatctg gataggtaaa
    18181 agttacatta tcaaattcta tgataccctt attttcagta tccttaattc cattttcagg
    18241 attttttatc aatggttctt catttaaaag ttcttttatt ctatttgcag atacctctgc
    18301 tcttggatac ataataaata ccattgaaaa aagcataatt gaaaacatgg catggaaaag
    18361 atattccaaa aatgcaacca actgtccaac ttgaagctct ccaatattta tcttctcact
    18421 tgaaatccaa aatacagcca aaacagctat atttaatagt aaaaagaatg ctggttgagt
    18481 aattgacata agcttaaata gtttcttaga aacattagca taatatgtat ttgttttttc
    18541 aaatcgctct gtttcataat catcatttcc aaaagctcta attactctta ttccagttaa
    18601 attttctctc gatataaggt ttaatttatc tagtcctttt tgttgttttg aagatattgc
    18661 ctgagatact tttgctatta ttgctactcc aataattata aaaggtactg atatagccaa
    18721 tactatagaa agttccacac ttgttctaat tgtcattatt atacttatga tgaacataac
    18781 tggagttaat aaggcagtcc ttagtagtat attaacaaat tgttgaattt gaaatgcatc
    18841 attcgttgtt cttgtaatca ttgaagaaat tccaaattta ttgtattcag tatgtgaaaa
    18901 ttcttgtgat ttctcaaata tgtcatttct tatatctctg gttacacttg ttgatacctt
    18961 agcagaacaa taacccaaca atatactacc tacaacccct ataatagaaa taataacaat
    19021 cactattccc atttgtttta tgtaagctat attctgattt gcaataccat tgtcaattat
    19081 tttagctatt atagttggaa tccccaactc gactaatgca aaactaaata tagccagtat
    19141 gtttaatact attaataact tataattctt taaataactt aaaattaatt tcatattttt
    19201 ctcctttctt aatttttgag atagtgtaat catgtaataa tactaaattt ataaatgctt
    19261 aattttataa aaaaaggaga aaagagttaa cgcactctct tctccagcat attcttttta
    19321 atacaccctc cgacaagtcg cttatatgtt aacatatttg acaagtaatg tcaattcata
    19381 tagctaaaag tcttttaaat gcttcatctc ataacattgt aacaaattgg catgtaattt
    19441 atttttgttt tttactgtat tccaatttta caagttctcc aatattacta actttcttaa
    19501 aattcaaatt agtcttatct ataccagact taaatagtga aatccctctc cccaatatta
    19561 ttggcaatat atatatataa taatcatcaa ttaagttatc tttcataaat aattttacta
    19621 tatcgcctcc accaactatc caaatatctt ttttatcctt cttttttcca atttgctcaa
    19681 ttaatttttt aggttctaaa ttagtatact ttacattctc cagttgtccc attaattgta
    19741 tttgagtata tatagcattc cttaccctta tagtaccatt catttgggct taattcattt
    19801 gcaatctgta catatgtgga atttcccata atgatagtat caattgtatc gtaaaaagta
    19861 ttataatcat cttcctgctc attcttaaat tctgaagata tctcgtctaa aaaacttaca
    19921 ctgccattct cttcagctat ataaccatct aaacttgtgg caatataaag tactactttt
    19981 ctcatgttta ctacctccaa catttatagt gtgattattc tatgtaaaca tcactttttt
    20041 actcattcaa taatacccta ttaataaatc ctcaaactaa tttaaataaa ctttgcataa
    20101 aactaaaaat atattatata aataatttaa aatcacacta acttattgaa aattctaatt
    20161 attacaaata aatattgctc tctgttaacg ctagttttat aatataatta taagctatta
    20221 atattataaa gccgatatta ctaatataat aattatagtt aatttacttt tgttgaaagg
    20281 tggtaaataa catgaaaatt tcagaagtcg ctactccaaa ttttttgcta gatttagacc
    20341 agttagaaaa gaatatagat aaaattcaac aaatttgtac aaataataat aaacaacttt
    20401 ggcctatgct taagacacat aaaagtactt acatagcgag acttcaaaaa aatgcaggtg
    20461 ctagtggttt tctagttgga acacttgatg aaggtgaagc tctgataaaa gcaggttttg
    20521 aagatattat gttggcttat ccttacatgg gagaagtaaa tataaataga attgtgacta
    20581 tggcatcaaa aataaattta atctgtgcta cagataatat agaatgtgct aaggcttata
    20641 gtgaagcttt ttctaaatca aaaatagagt gcaagctact tattattgta gattgtggct
    20701 tacaccgttt tggggttgaa ccagaaaaag tggttgaact tgctactgaa atttctaagc
    20761 tagaaaacat caagattgtt ggtataagtt cgcatccagg tcaagtgtac ggttgtagtt
    20821 ctccagaagg tgttccagat gtttgtgttc aagaagattc ttctatggag aaagcataca
    20881 aagctctaac tgataatgga tttaatatag aaattgtcgc ttctggtagt actccaacag
    20941 ttgaagctga agctgcttct aaaactataa ctgctgtaag acctggaaac tatgtatact
    21001 atgatgctat acagataggt ttaggttgtg ctactgaaga tatgtgtgct cttactgttg
    21061 tgtgtactgt aatctcaaaa aatagcagtg gatattattt aattgattgt ggaagtaagt
    21121 gccttggact tgataaaggt gcacatggaa atagtagtat tgttggttat ggtagggtat
    21181 gtggacatcc agaacttact attgattcac tttcagaaga agttggaaaa attaaagtaa
    21241 atggtgaaac tgatataaaa attggtgata gaataagaat tatacctaat cattcttgtt
    21301 ccactgcaaa cttgacgagt aatttattag gttttagagg agatacaata gaaaaagtca
    21361 tagaagtaga tattcgtgaa aattcaaaac aaatcatcct ttaaaaatat atttattttg
    21421 atattaacat catgaaagta tatttttctt aaaattattg ttaataacta atgttaatgt
    21481 tggataaata aaaaacataa attctagaaa tataaattta aaaaaagagg ctgtctcaaa
    21541 attggatatg tccctgtcaa gtagacagac ttaaaaaagg ctatctttat gcggaatgag
    21601 ttcgatattc aatcggactc attccatttt ttaattgtat tctttcatta ttataaaaat
    21661 aaatataatc atttattaga ctaatcattt cctttggatc tgtagtatca attaaatata
    21721 ttaattcaga tttaaaatga ctgaaaaaac tttctatagg agcattatcc cagcagttgc
    21781 cccttcggga catagattgt ataataccat tatctttaag ccttttagag taagatctac
    21841 ttgtatattg tgaaccttga tctgtatgta aaatacaatc tttagctaaa ttcatattta
    21901 ttagttgatt tactgtttta ttaactaatt tagtatccat ttttgtactt atctcatatg
    21961 ccactatttc accattaaat aaatctttgg ctacattcat gtataagaat tttttaccaa
    22021 taggtatgta tgttatatcc atacaaattt tttctaatgg ttgattagct ctaaattcac
    22081 gatttaatat attatcatat acctcatatg atttaaactt acgtctatat actttctttc
    22141 taataataga tttaattcct agttctttca tcaacctata tactttctta tgattaatag
    22201 ggctatcaag tattttattt aaataagtac aaattctttt tctaccgtaa gttcctctat
    22261 attttttatg tatttcaatt atataatcct taataattct atctttgata cccctatcgg
    22321 aagatttttt agaataactt agccatttat agtaaccgct tcttgacaca cctatcaact
    22381 tacacatact agaaatacta tacttcctag acataagttt aattgtttta aatttatcgc
    22441 ttactttgac ttgtccaata gggtacttaa cttttttaaa aattcattct ccatgcgtaa
    22501 gtacttcaat tcttcttcaa cagatttaaa ttttctatta tcattttcta gactaatatt
    22561 tttaaatctg cctctagttt cctcattaaa tgcagtttta c
//
LOCUS       DACTGW010000046        22047 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.046, whole genome shotgun sequence.
ACCESSION   DACTGW010000046 DACTGW010000000
VERSION     DACTGW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.046"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(126..1049)
                     /locus_tag="I9573_001371"
     CDS             complement(126..1049)
                     /locus_tag="I9573_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4795997.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(1303..3003)
                     /locus_tag="I9573_001372"
     CDS             complement(1303..3003)
                     /locus_tag="I9573_001372"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAT4795998.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(3414..3827)
                     /locus_tag="I9573_001373"
     CDS             complement(3414..3827)
                     /locus_tag="I9573_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAT4795999.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKIKSEKDL"
     gene            complement(3895..6273)
                     /locus_tag="I9573_001374"
     CDS             complement(3895..6273)
                     /locus_tag="I9573_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAT4796000.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSEISAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRNLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            6590..8041
                     /locus_tag="I9573_001375"
     CDS             6590..8041
                     /locus_tag="I9573_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HAT4796001.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(8245..10758)
                     /locus_tag="I9573_001376"
     CDS             complement(8245..10758)
                     /locus_tag="I9573_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAT4796002.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTSDEQLEVLKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNADKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLEDMAKKHEIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLNGK
                     RIALAKGIDYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYEAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKSDKESENGNIENERDKIRREYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRITSKIIYNDRKIPMYVIGKIANIDSEKEEKNRLLDKVQR
                     DSLTNIYNAAYIRKLTTQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLVGDKVLIDV
                     AKVLDESFAKDDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVITKRGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(10981..11802)
                     /locus_tag="I9573_001377"
     CDS             complement(10981..11802)
                     /locus_tag="I9573_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAT4796003.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMIVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEMR"
     gene            complement(12321..12797)
                     /locus_tag="I9573_001378"
     CDS             complement(12321..12797)
                     /locus_tag="I9573_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HAT4796004.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVGNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(12831..13409)
                     /locus_tag="I9573_001379"
     CDS             complement(12831..13409)
                     /locus_tag="I9573_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAT4796005.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(13513..16131)
                     /locus_tag="I9573_001380"
     CDS             complement(13513..16131)
                     /locus_tag="I9573_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAT4796006.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIETGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MNRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMASSISIDSSISIDSSISIDSSISMDLDTSSSLGIPKELMGSNTNNIDTLSNTSK
                     IDTLNKNHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFK
                     KKDLNIDCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGY
                     APMMVTEYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSK
                     VTCMLDNLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKK
                     LDEIGTVAGHYYKGVE"
     gene            complement(16623..17306)
                     /locus_tag="I9573_001381"
     CDS             complement(16623..17306)
                     /locus_tag="I9573_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAT4796007.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(17648..18238)
                     /gene="zapA"
                     /locus_tag="I9573_001382"
     CDS             complement(17648..18238)
                     /gene="zapA"
                     /locus_tag="I9573_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAT4796008.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDVYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(18263..20656)
                     /locus_tag="I9573_001383"
     CDS             complement(18263..20656)
                     /locus_tag="I9573_001383"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAT4796009.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(20673..21692)
                     /gene="pheS"
                     /locus_tag="I9573_001384"
     CDS             complement(20673..21692)
                     /gene="pheS"
                     /locus_tag="I9573_001384"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAT4796010.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
BASE COUNT         6800 a         4030 c         2393 g         8824 t
ORIGIN      
        1 aaatactcaa attagtgatt tcattgacct taggaataat atagtaaagg aattatgttt
       61 tttaactgac actaaggaaa aagatttttt agattatcta gaaaaaaata gcacactcta
      121 ataacctaaa cctttttaaa ctttaatata taaatacctg atttagaatt tatttttatg
      181 cttaatatat tatcattaaa ggttttcgac ataactgtta tttgattaaa tatagggtct
      241 attatagtat taccttcttc aagtcttata agacttcttg ggtctatgct tttattatat
      301 gccttaactc caattttata ttttctcaat ttaacaacat attcggacat agaaaaaata
      361 aaatctatat tatcttcatt taatttacta tcttcaccca aaatggataa tatagatttt
      421 tttatgctat taattccttc attttctaca gttctatctt ccatatatac atcatataga
      481 acatttaaaa catttaataa aatactttct tcatcataat cttcagattt caaatcaatg
      541 tacttatcaa ttaattgtat tgctttaatc cttgccatct gaaactttac aacatctgat
      601 ttgtctaatt ctatagaaaa tcctattatc ttgtctttaa tactctgtag taattcgaca
      661 tactctattt tactattatt tataagattg tgcataatgg aattatacac tacagaacct
      721 aataaataat caaataaaag ttcttctttt ttaaaataaa taacatactt ttttattagt
      781 ttaataacct catcttcgct tttttgaata ttagtatttg ataaaattag tggtagtaaa
      841 atatttaaaa aattgatatt tcctattttt tctccataaa atctatagta aataagttca
      901 tctctcagtc tattttcgtc tatatacaca catctttgcg ttttatcatc tattaaggat
      961 tttgtattta ttatagttaa atctttaaga gccctacttg cactactaaa agaagatgtg
     1021 tagacttttt ttagtaattc gtcattcata aatatatcct ttctgaaaaa ttgaatgtaa
     1081 ataaattgtt attaaaaaaa aagagacagc ccgtttaaga agctgtcata tataggggga
     1141 tagtagtgta caaaaatcat tgtaatctac tatctatata ttattatacg atactacttt
     1201 tttatatgta aagcatcagt tttcgacatc cctatatttt acagttatac aaattgcgta
     1261 ctaagttcaa ttttattctt tttatttcaa cattttatca tgctacattc tttccggagc
     1321 gtgcattccc aacaatttca gagaattttc taaagtttgt tttgttgcta aaactaatgc
     1381 aagtcttgct tttcttattt ccaaattttc tactaaaatt ggattgtcat gataaaattt
     1441 attaaacgct tgagctaaat ctaacataaa tctagtcact atatgtggct catttttctt
     1501 tagagcaagt aaaatattct tattgaatga ttctataact ctaagaactt ctgcactatc
     1561 ttcatcagct aatagactat aatctatatc agaagtcact tctacttctg cttttcttaa
     1621 aactgcacag catctagcat gtgtatactg aacatatggt ccagtctcac catcaaaact
     1681 aagcgttctt tcccaagaga aggtatagtc tttaattcta ctgttagata actcttggaa
     1741 tactactgcc ccaacaccta cttgcttaga tatttcatcc acatttttag catttggatt
     1801 tttagctagt ataatttcct tagttttatc tatagcttga tttaaagcat cttctaaaaa
     1861 tactactctt ccttttctag tcgacatagt accttcttca agtgcaacca taccaaatcc
     1921 aacatgaatt aaatcttttg cccaatcata tcccataagc tcaattacct taaaccattg
     1981 ttggaaatgt aagttttgtt gagaaccaac tacatatata cacttgtcaa aatcataagt
     2041 ctcttttcta tatattgcag cagctaaatc tcttgtcata tataacgttg aaccatcatt
     2101 cttagtaata agtgctggtg gcattttata ctcttctaaa tcaacaattc ttgctccttt
     2161 agactctttt aaaagatttt tctcttctaa tagttcgata actctgttca ttttatcaga
     2221 ataaaaactc tctcctgcta aagaatcaaa ttctatatct aaaagtttat acactctgtt
     2281 aaactctttt aaactctcat ttctaaacca ttgccataat tcttgtgctt cttcgtcacc
     2341 attttctaat ttattaaacc atgctcttgc ttcatcttcc atttctgggt gttgttcagc
     2401 ttcatcatgg aatcttacgt ataattttag taattctgga attggattag actctactac
     2461 ttctttttct ccccactttt taaaggccac tattaactta ccaaactgag ttccatagtc
     2521 acctaaatga tttattctta tagtcttgta tccttgcgaa tcatatatct tatataaagc
     2581 attacctata actgtagttc ttatgtgccc tatatggaaa ggttttgcta tatttggaga
     2641 agaatattca actattacag ttttatcttt tccaaaatca ctatgtccgt aattttcttt
     2701 ttcatctaaa acttttttaa ttacagtttc tgctaattga gatttattta caaagaaatt
     2761 tacatatccg cctaattgta ttacttttgt tatctctcca cttggttcta tactttccgc
     2821 caattcactt gctatcatgt ttggagcttt tctaaacact ttggctaatt taaagcatgg
     2881 aaaagcatag tcccccatat ccttatttgg tggaacttct ataagtgcct ctatctcttc
     2941 tttcgataaa tcttctattt tttctttaag gcaatttgat attgccacct taaaatcttg
     3001 cattctgttg ctcctctcta cattgttcct ggtttatatt gtgcttatct ctttatgtta
     3061 aatcaaattt cgtgtttttt caagtatttc attacagtgt aaaacacttt aataattatg
     3121 ttttctgtat atattaaaaa tactataatt gcagttttta atcacactat tattgatttt
     3181 ttataagact tttttctatt ttattgtttc ttgtactatc cagttgactt tatatacttt
     3241 ttttaatctt ttatcaattt tcatacatca atattaattt tatatacatt tattttgctc
     3301 ttttatctat ttttatacat caatattagc tttatatact ttttttactc ttttatctat
     3361 ttttatacat caatattagc tttatatact ttttttactc ttttatctat tttctataaa
     3421 tctttttcac tcttaatttt tattccttct tcttctaaca gccttgctgt tatacctttc
     3481 cctggtatct ttttacctga aaaactacca tcatatatta gattacatcc acaagagggg
     3541 ctaccctctt ttagtattgc ttcgctacat acatataaat ttgctatttt tagagtttca
     3601 ttagcccctc ttacaaagtt ttctgttaca tccttattat ctttagtaat tacttttcca
     3661 tcaactatct cagcaggtgg tcttggtgta gatagaccac ctaactgctc tgggcataca
     3721 agtgtaaatt ctttgccttt taaatattcc ttgacttttt cattttcatt attatcgcca
     3781 ctatatttac aatttattcc tattaaacat gcagatacca atatcataaa aatgtgctcc
     3841 tatacaaatt cttaatatat tcttatacaa attactaatg tattcttata caaattattt
     3901 taatttaaca attgttactc ctgctccacc ttctccatat tgaccacctc gttgtgattt
     3961 tacatgtcta tttcttctta aaatgtcttg tagccctgct tttaatactc ctgtgcctat
     4021 tccatgtatt actgttacag actctaaacc tgccacatat gcatcatcaa gatacttttc
     4081 tacttccatt atagcttctt ctaggtttaa ccctctaagg tcaacttcat tttttacact
     4141 accagatttt gaccttataa tgtttctcgt tgatttagtt acatttgttg aaacatcttt
     4201 gcgtgttttt tgtaaagatt taaaaggtag agtcatcttc attataccaa tttgcactac
     4261 tgcttctttt ctctttttat ctactgaaac tacactacca ttttgattta aagttattac
     4321 cttaacttct tcacctggct ttaagtcttt tatctcctta tttgatacct ttggaactat
     4381 catacttttt actgttggct gaagacttcc catagagctg gtcaattctt ttctaagttc
     4441 ttctatctta cgattctttt cttttgaagc tctttcttgt tcaagatttc taagttcttt
     4501 tattataata tctacttctt ctttagcttg tcttataatt gaaaatgctt ctgatttagc
     4561 tttttcaatc atcttatctc tttgactgac caatttttca agtttttcgt catactctac
     4621 tttcagtttt tcaatctctt cttttaatct ttcagcttct tctctatctt ctactgcttt
     4681 gattctattc ttttcaacat tctgtaacac atcttctaaa gctatgtttt ctgtatttat
     4741 atattcttta gcacgtgaaa tcacataatc acttagccca agttttctag aaatctcaaa
     4801 cgcatttgat tttccgggaa cacctataag taacttatat gttgggctta aagtttctat
     4861 atcaaattca actgctgcat tttctacacc tggtttagta agagcataat ttttaagctc
     4921 actatagtga gtggtagcta tacatttagc tcctacagaa tttatatctt ctaatactgc
     4981 tattgcaagg gcagctcctt caacagggtc tgttcctgct cctaactcgt caaatataac
     5041 taaagagtct gctgttacgt tttgtaatat agatactata ttcgtcatat gagatgaaaa
     5101 tgttgataga ctctgttcaa tactctgttc atctcctata tctgcaaata cattatcata
     5161 tacacacata ctacttccat aatcagcagg tatatgaagt ccactttgtg tcataagtgc
     5221 aaatagacct acagttttta ttgtaacagt cttaccacct gtgtttggac ctgttatcac
     5281 caatgtatgg aagtctcttc ctaaataaat tgtatttgct actaccttct ttttatccag
     5341 cagtggatgt cttccatttt ttatgtttaa atatttatcc tcatttaaag ttggttctat
     5401 tcctctcatc tgtatagaaa gttttccctt tgaaaatgca aagtccaatc ttccaagtat
     5461 ctcttgatta gagattaaat cttcgctcac ttctccaacc atagctgaaa tctctgataa
     5521 aattctttct atttcctctt gctctcctag tttcaactgc ctcaattcat tgttcatctc
     5581 tactatagtc attggctcta taaaaagtgt cgcaccagaa gatgactggt catgcactat
     5641 accagctact tgcgacctat attcagattt tacaggtact acaaatctat cacctcttag
     5701 agaaattatt gcatcttgta agtacttttg ataagtagtt gaacttatta ttgaatttaa
     5761 ttttgaccta atggattgat ttttttgggc tattcttctt ctgatatctc taagtgtact
     5821 tgaagcattg tcagatattt ctacttcact aattatagca ttgtatatct ggtcttctat
     5881 gtccttgtac acatatagag aatttgatag agattgtatt attggataat taaaatcttc
     5941 ttcatcacta ctagataagc tatttcttaa tcttctagcc actcttaagg tatctgctat
     6001 cataatcaga cttcctgggt caagagaagc acctatataa gctctcttta ccttatcttc
     6061 tatatcatga ataccttcca accctactga acctctcttt ataataattg attgagcttc
     6121 actagtttct tctagcatag atttcacttc tacaaaatca gtatttggta ctaaattttc
     6181 tatatatttt agacctaaag aagatgaggc tttcttcttt aacaaatcta ttattttatt
     6241 gtattctaga actcttaacg atttctcatt cattttctat attcaccatc ctcgatattt
     6301 taactttttt atttaatttt aaaaatgtat gttttcaagc atttccaata aaaaatgcaa
     6361 gttttatttc tttttcattt ctaaaattat tatatttttt ctcttttaga tatattttac
     6421 acatattttg tattatacta tgtatgtagc tatctatata ttatacataa acatgcttta
     6481 tttgtaatat aatctgttaa aaaatcttct ttaatatttt taaaggagtt tattttagct
     6541 tataaaaaaa tagctactta tatagataaa ttaaggagga acatgagtca tgggaaacgt
     6601 attagaagga ttaaaaccag aaagtgtttt taaaaacttt gagaaaatat ctcaaattcc
     6661 tagaggttct ggaaatgaaa aaggcataag tgatttttta cttagctttg gtaaaaattt
     6721 aggtcttgaa actattcagg atgaaagctt aaacataata ataagaaagc ctgctacaaa
     6781 aggatatgaa aattgtccag gtgtagttct tcaaggacat atggatatgg tttgcgaaaa
     6841 agaaaagaat gttgaacatg attttcttaa agaccctata aaattaagaa ttgatggtga
     6901 catgatatat gctactggaa ctactctagg agctgacaat ggtatagctg ttgctatggg
     6961 tatggctata ttagaagata atactttaga gcatcctgca ctagaagttt tagttactgt
     7021 taatgaagaa gatggtatga atggtgcaga tgcactagac cctagcttaa ttaaaggtca
     7081 atatatccta aatatggact ccgaggagga aggatactta ttagttagct gtgctggtgg
     7141 taaaacttgt gttgttagtt tacctgttga atacaaggaa gttaaaggtg acaaacaagg
     7201 attattagtt gaggttactg gactattagg tggacactct ggtatggaaa ttgttttaca
     7261 aagagcaaat gctaacaaag caatagctag agtattaagt gtattgaatg ttgattatga
     7321 attagcttct gttgatggtg gtactaagca caatgctata cctcgtgaag ctaaatgtgt
     7381 aatagctgtt aataaagctg atgttgaatc tgctaaaaaa caaataaatg acattttaac
     7441 tgcatttaaa catgagttta ctacttctga ccctggcatg acttactcag tagctgaaac
     7501 ttctgtagac aaagttctta ctaaagattg taaagaaaaa gttgttcaaa tgtcttgctt
     7561 aactcctcat ggagttcaat cagtaagtct tgatatagaa ggattagttg aaagttctac
     7621 taacttcgct ataattgaaa ctaaagaatc tactattgaa tttttaactt ctgttagaag
     7681 ttctgttatg tctattagag atgaaatcgc tgatagaata agacttttgg ctcaagcttt
     7741 aggtgctaat tacgacctta tagctcaata cccagcttgg gaatttaaaa aaggctcaaa
     7801 attagaaaaa atatgcagtg aaacttatga gaaattaact ggtaaagttc ctactgttat
     7861 ggctctacat gctggtcttg agtgtggatt attacttgat aaacttcctc atgctgaagc
     7921 tatctctata ggaccagata tgtttgatgt tcatactcca aatgaacatg ttagtatccc
     7981 ttctgtagct aatgtttggg attatgttat tgaaatatta aaatctatga accaatatta
     8041 aatttaagtt ttgattctaa atagatttta attttaagta atctatataa aattaactct
     8101 gtaaattaat actactgtta gtataatatt tactttcaat attaacttat agaaataaag
     8161 cctaatgata gataataaca tgtatcatta ggctttattg taatttaaat attattaaac
     8221 tttattctgt tttaaaatta attcttatac tattttaaat ctatttcttc cttgtctctt
     8281 agaatcataa agagcattat ctgctatttg atacagttca tcatactctt gaccacgctt
     8341 tgttataact acccccatac taatagataa actttctcca tttggcaact taatgagatg
     8401 aatatgattg tatacatcat tacatttatt gataagttct tccttattta ttatatcatt
     8461 tatataaaca ctaaattcat ctccacctaa acgacctact atatcatctt ttgcaaaact
     8521 ttcatcaagt acctttgcaa catcaatcag tactttatct cctaccaggt gaccataagt
     8581 atcattaatt gacttaaaat aatcaatatc tataatcaat aaagctccat ttttttcagt
     8641 tgataaattt aataaatttt gtgtagttaa ttttctaata tatgctgcat tataaatatt
     8701 tgttaaacta tccctttgaa ctttatccaa caatctattc ttttcctctt tttcagaatc
     8761 aatattagct attttaccaa ttacatacat tggtattttt ctatcattat aaattatttt
     8821 tgaagtaatt ctaatccaat gaaaagtatc atcaagcatg atagaataca tttcagtagt
     8881 cccattttct tgtgatttta ttacatcaaa aaacttttta ttatattctc ttcttatttt
     8941 atctctttca ttttctatat tgccattttc actttcctta tcacttttat ccaaaccata
     9001 ataagtctca ataccctttt ctggcataga aattatcaat ttatcttgct tataatcata
     9061 ctcaaaaaag tattcacttg tcatttcata caactgagta tgttttttga gttccaaaga
     9121 tatcttttcg ttcatacttg tacgagtatg taaaccaaat gccataaatc caataataca
     9181 aattaataaa cttgctacaa taaaaatagt ctcaattgga ttctgttgca taatataaag
     9241 caacgagaaa tcttgtctat atgtagtatt ttggtagaca attgattgca ttttttctga
     9301 tgaaatactt ataatagctt tattaaatat attcaataat tcttgtttat cttttttaat
     9361 tatgccaaaa caaatactct ttgcttcata agtttgagga actaggcgca catttttata
     9421 cttaggttga ttcatatagt actgcataga ataaccttct ccataagtgt aatctgcata
     9481 gcctttatta acagcttcta tacactcttc taaattatca taccaaacta tattttcttc
     9541 aaactgacca taataatcaa tacctttggc tagagctata cgctttccat ttagactatt
     9601 ctcatttaat ttattattaa tcatcatagt atattgagta gaaagaaaag gccgagacat
     9661 agctatatta tgttcacgag agtactcata atcataaatc attcctgcta caatatcaat
     9721 ttcatgtttt ttagccatat cttccaaatc ttcttgagat tttacaggca caagttcaaa
     9781 ttccaaacct gttttatcac atatatgatt taacaaatca attgatatac ctcttaattc
     9841 tcctgtatct ttatctgcat tttgaaacgg tggtttgttt aaaagtactc ctacttttaa
     9901 tttttctgta tctttaatgt aattagtttc ttcatcagat aaaaacattt gattattctc
     9961 tggcgtaaag tatttctcat ataaagtagt tgaaaaatat gggtccttct gttcaatatt
    10021 attaattgct gaattaagct tttgagttaa gtctgtactg ccttttggag taatgaaata
    10081 aaatggcttt gatgagaatt ttgctacagt acgcacacct tctattgggt tcatactaac
    10141 attaagaagt acatctgctt taccactctt tagtacttct aattgctcgt cacttgtttt
    10201 acaatatacc aattcaggtg acataagatt cattttacaa tactcatcta attctttaag
    10261 cctagtatca gaattttcta ttacagcaat acgaattttt ttcacatctt gcgaactcat
    10321 tatgttatca atatctgtat tttcatataa tgcttgtagt actgtatttg ctactccata
    10381 actgtttccc gcataattat atattttttg cattggctca ttgtataaca tacctcccat
    10441 tatatcaatt tcaccatttt ctaacatctt cattaatttt gtaatagatt catttatctc
    10501 tccaggaact tgaacaaatt cataattcca tccagtatac tgtgcaattt ctagcaaata
    10561 ttcatatgta tacccaaaat agttaccttg ctcatctatt tcagttaaac ctttttgaat
    10621 aggataagca acacgaatgg tacgattatc attacctata gcattgatac caaaagaaaa
    10681 tatgcaagaa gtgaatatag atgtaattac aataattaat aaaagtaatt tttttaaatt
    10741 cactacacaa tcctccatat agttaagatt tacagtataa attactgcat aaacttgtag
    10801 acatgaaaaa atagattagt atataaaagg atatataaat ctacttagaa atttaaatat
    10861 ccttaatata tacaatatat ttaaattata actcatttat atatctatta caacaaattt
    10921 accctgctaa atacctactc tatatcccat aattttacaa gttgtaatga agtttaagtt
    10981 ctatctcatt tcaaatttag cagtgtttct atttacagtt cttttatatt tcacatctgg
    11041 ataacccaat ataaatgaag tgataactgc ttcgttctcg ctcattccta acagttcttt
    11101 tatctttggc tctacatttg atgccataac aaaaaatcca ttataacaaa ttcctaatcc
    11161 ttgagaatag gccataagtt ccatattaga tgcagccaat cctccatcta cataagcact
    11221 tccacctaga gacatatcac ctacaactat cataagtagt ggggcatcat aaaataaaga
    11281 atcatttccg ttctctttat atcttttgta cattttctta aatgtatttt tatagataga
    11341 acgtacaggg tcattgtcat cagtatctaa tgccaaatta taaagacctt gtattgccag
    11401 ttctttcact tcttttagct tttcttttac taatatgtat cttataggct gtctattacc
    11461 acctgttggt gtgtatctac cagcttccac tatgtttttt attttttctt cttctacctc
    11521 tatatcttta tattgtctta tacttcttct aaatttaata aaattcaata acttttcggg
    11581 ttctaattca aaactttcac gattgtattc tattacttca tttttatcaa atccttgcat
    11641 ttctattgca tctgttgggc aaattgccat acagtgacca cagttaatac agagaatatt
    11701 tttaaccttt gctttatcat ccttaacttt tattgcattt acaagacagt ctgattcaca
    11761 catcccacag cctacacatt tttctaaatt tatgtctatc attccaaatt attcctcctt
    11821 ttaaaaaatt tactttagta taaaacaaat agttgtctaa gtcaattttt atttttttaa
    11881 caatatacta gccttaacta cactttaatg gagaaaaata tacataaaat tagaacttaa
    11941 attagaattt attcaaaata aaagataaaa gaagtaaatg tctaaaagtg aactttttag
    12001 ttaatgagac attctttatt gtatgaaatc aagtggatat ttatcatttc aacaataaaa
    12061 aaggcttacc tctattttgg atatgtcctt gttaagtaga cagactaaaa aaggctatct
    12121 ttatgcactg agatagcccc ttttcccaac tactattgtt tattttaata ttatatcaat
    12181 aaatttaatt tgctataacc tgtaagttac attttaattt gatgtaactt taaattacat
    12241 ttttaagtaa tatctttaaa ttatattctt aaaatatatc attaaactac attttaatta
    12301 atctaaaaac tatatcttaa ttaatctaag aaacttacat tataatcttt tagtttccca
    12361 ttatcatata caatctttgc actaaagata tttccagtct tagcccaatt taagaacaac
    12421 ctgtcacctt cccacatatt taaatcgaat attttatcat taccaaccca aattaaattt
    12481 ccttctttac aatcatttaa atctttttca gacaattcac cttcaaactc atctgagaaa
    12541 tacaaaaaca tataccaatc ttcttcatct ttaaattttg gaaaagttat caacccttta
    12601 tatgacaatg atttaagagt aagaccagtc tcttccttta cttccctaat tacacattct
    12661 tctggagtct ctccttgctc aaactttcct ccaactccta catacttacc ttcatgtata
    12721 tcatctttct ttttatttct gtacaacatc aaagtcttgt catctttttc tatataacaa
    12781 atagttgtaa gtttcatgta tctcgcctta ctttctcttt atattctcaa ttaattattt
    12841 tcagcacttt caagcacatc acttatattc ttcattgcat taagagaaag ttctgcaaat
    12901 tcatcaaatg gaatccctat atcttctata cttctcattg catctctatt tacatttgaa
    12961 gcaaatctcg tttctttcat tcttttaata acagacttag tctttacact agtaagtttt
    13021 ttatctggat aaactaaagc tatagcagtt ataaacccag ttatagggtc tgctgcatat
    13081 attgcttttt ccattaagct agttcttgta actcccattc catcatgatg accaagtatt
    13141 gctctataca tttcttcgtc accgaagttt tcttttttaa gcagttctac tgttgttgta
    13201 gcatgtcctt cacatatttc tcctggcttt cttccaacta tatctgaatc caaatcatgt
    13261 aaaagacctg ccactgccca ctcttcttct ttttctggct ctagtctttt ggctaattct
    13321 ctcataacag cttcaacagc ataacaatgt tttctcattc tatcagtttc taaatactta
    13381 aataaaattt ctaaagcttc ttctctcatt ttcaccaatc cccctgtttt ttcacatata
    13441 tgatattttt atatattaat acacataaat tcaagtcttt atatatattc atgtatattt
    13501 ttcatgttat atctattcta cacctttata gtagtgtcct gcaactgttc caatttcatc
    13561 taatttctta tacaaatctg tagccttttt tccaagttta taattattac tcaatacctc
    13621 aatgtgagct tcaataacct ttttaaccat ctcactatct tcaactgtaa aatctatcct
    13681 aaatacatct attccaattt cctctaactc atatagattg tccaacatac aagtaacctt
    13741 cgaattgtaa attgtacttc tacagaatat atcttgtgtt atcctataac ttgcatccgt
    13801 aaagtctctt aaagcataac tactttcctt acatttagca actctcttgt cctttttaca
    13861 atctctaaca actactccca taggacagta ttctgtaacc atcataggag catatccata
    13921 tattactgct tcaatttcta catctgtata ttttaaagtt tgtttaattt catttaagtt
    13981 tagttcttgt gacaaacaaa ctgtacttgc tccctcttct ttgaagtggt ttatactttc
    14041 actattgaat gcatttaagt agcaatctat atttaagtct tttttcttaa agtattccat
    14101 agagccccag ttaccaattt gaaccttatt aattcctttt atattatcca atatctcaaa
    14161 ttgcttgtac tctttatttc taacaatcct tggtgctgaa tagattaatt ttttgtcagt
    14221 gactgctgta ttatagtggt tcttattcaa agtatctatt ttacttgtat ttgacaaagt
    14281 atctatgtta tttgtatttg aacccattag ttctttagga atacctaaag acgaagaagt
    14341 atctaaatcc atagaaatac ttgaatctat agaaatactt gaatctatag aaatacttga
    14401 atctatagaa atacttgagg ccatttgtaa agcttttgat aatgtattta tatcttcata
    14461 atagattaaa tctacattat aatttaatac tgcttctaat tgctccaagt tcttaacttt
    14521 aactctaatc ttaggaactg tatcaagttt tttctgttta tttactctat ttggttggta
    14581 ttttaatcta ttcttcttaa attttctatc ttttatcttt attctttctt catctaaaag
    14641 ctcaatacaa tcccttctca tttgatttaa cacacttata ggcatactta caccttcatc
    14701 caggtcaatt tctatctctt ttaacttata tggtgtattt ccaagctttt gaatttgtgt
    14761 ttctattttt tctttactta aagccacctt tatagctttt tcaactattt tgtctccttc
    14821 taccaccgat ttatttccac aaaaatcact tataaaaagc tctggctttt tacctagttt
    14881 aatactcact tttgcacgta tattagtttt tatgaactct ttatcttgct gaaatgtagc
    14941 ttgtactcta ttcattaatt cgctgtcaga agtcttatat ataagctgat ttctctttgc
    15001 ttctccaaca aaatctaatt ctatcaaatc ccctttatgt gctattgtag ttatctctcc
    15061 atcctttatt attcttccaa tagtacctcc acctaagtta attccatcac ctttcttaag
    15121 cgtattttct agcattactc taagtctttt gttctttcta ttataatcta aaacttttcc
    15181 aatatatagc ccttgattat taggtctatt agagttcatt atatccttac caacttcacc
    15241 taatatatat ccttttgtaa actttctatt aaatatggta tacaaattat ttattatttc
    15301 ctcagaaata cttatttttt tcttatctac aaattcatta atcgcttctc tatatcctga
    15361 aaccacagta gctacatact ctggtttttt cattctccct tcaattttta aagataatac
    15421 acctgtttca attattttat ccaaatcttc tatagtactt aaatctctag gactcaacaa
    15481 ataatctcca ttacttttta ctacttctcc tgtatttata tctatcaact catacttttg
    15541 tctacatggc tgtgcacacc tacctctatt tccagaccta ttgcctatca tactactcat
    15601 aaggcattga cccgagtaac atacacataa agctccatgt acaaaaactt ctatatctac
    15661 agttgtattt tcacaaatcc attttatctc atcaacattt aattctcttg ctaaaacaac
    15721 tctatcaaac cctacactct gaaggtattt tacatcttct aaggaatgtg ctaccatttg
    15781 agtgcttgca tgtagttcaa agtcaggtaa tagttttttt ataagtcttg ccattcctat
    15841 atcctgtact attaaagcat caatatctat atcgtataaa aattttacat actctacaaa
    15901 atcttctatt tcatactgtt ttatcaatgt atttacagta acaaagactt gcactcccct
    15961 tatatgagag tatttaacag cctcaatcag ctcatttcta tcaaagttat ttgcagatgc
    16021 tctagcacta aagtctttac cacctaaata cacagcattt gctccgtttt gaactgctgc
    16081 ttttaatgaa tcaaaagagc ctactggtgc aagtaattct atttttttca tatttttctc
    16141 ctctatttat acttgttagg ttaatataat tatatctata acttaaatat ttgtattaaa
    16201 tttatatatt tcttactata ttttaaattc ttcaattaat ttatatatta gttatttata
    16261 tttggtccat ttgagttaac atatacatta tttactatat ttagtatatt tctattaact
    16321 tatatattga ttactgtatt ttaaattctt caattaattt atatactgct tactaaattt
    16381 aaaacatttt aattaatttg tacactgttt actatacttg aaatacttct attaatctat
    16441 atattgccta ctatacttaa gacattttaa ttaatttata cactgcttac tatacttaaa
    16501 atacttctat taacttatat attgcttact atattttaaa ttctttaatt aatctataca
    16561 atatttgcta tatataaagt atatcaaata acagcttcat ttttttgtaa atttacagaa
    16621 agttaatttt cattaaatga atcatcttta ttgataaaca taattccaaa gaccactgcc
    16681 aaaataaaaa aacatattag tattgatttt tccacgaaaa tttttgtaaa aaaagttcct
    16741 aaaatagctc cagatataaa gaatataata atagcataat actgtaaact cttttttatc
    16801 ataaaaatat tcttagtatg tatataatga aataaagttt ctgtagcact tcttaaattt
    16861 ccagtacaca ttgtcgttgc aaaactattc ccatttactt ttctaaaact ctctacttgc
    16921 attgaacaaa taaaagaaat agcaacattt acaaacatat taaatcttcc tctaggaata
    16981 aatgaaacaa taagtattgt aatcatttca attataatga taacctgacg ccaatgaatc
    17041 tttgtcttat tatttgtaaa atactttctt atcttctcgg ctataaatac acctaacata
    17101 aatgccaaaa taggaatcaa ataataaaat gattccttta cattaccttc tgctatatta
    17161 agcccaaata aaactatatt tccagtttgt gcattagcaa agactttacc tcttaccaca
    17221 taagtataaa tatccaaaaa tcctcctgtt attgccaaca aagaacataa aaagaatgat
    17281 tcagacatct gactcttgct tgtcatatat aaacctctcg ctttctaatg atagatacaa
    17341 aatcaaaatc attttagatt ctatttttca ttgatataaa aaaattattt atatttaaat
    17401 attatctata tttatacatc atttatattt atacattatt tgtatttata tatcattcat
    17461 atttatttat atttaaacat tatctatatc tatatagcat ttatatttat ttacatttaa
    17521 acattatcta tatttatata gcatttatat ttatttacat ttaaacatta gctatattta
    17581 tatagcattt atatttatac attaaaagaa gctgtctcaa aaaacaagag acagcaaatc
    17641 ttttagttta ttccacattt tttgcatcat tatttaaccc tgtacatttt aactgtaaat
    17701 cataagcttt gttttgaaac tcagaagcta agttttcagc tatagtagct ctttcatttg
    17761 cttcttcaag ttgagtactt aactcctcaa tcttgttttt ataaacatct aactctgctc
    17821 tagaaccttc tgtcgtatta ctaagttcga atatttcttg ctttttattt tctataatct
    17881 caattaactc tttcatctta gtctcagctt cacgattttc tgcttctttt tctgctatcg
    17941 taagctttaa agacttgatt tctaattgaa gctcttcatt agatgctcca accttcttac
    18001 tcaattcttc attagcttta atcaacttct cattctcatc tgaacattca aataaaaggt
    18061 ctgatatatt taatgcagtt agtatcgcag caactgataa gctcaatttc gggttttgac
    18121 gtgttatttt atccatttct ttgtctacaa aatcagcaat actaatcata aacttctctg
    18181 atttgtctcc aaccattgga tattcagcgc catggatttt aaccattact ttgttcatcc
    18241 taacctcccc cgacattagt tgttaatttg atcttaagtt agcatttaat ttttcgctta
    18301 attcacttaa tatcttatca tgcaccttag ctacatcttc atctgttaga gttttatctt
    18361 tacttctata agttattgaa tacgcaattg acttgtatcc agcttctatt tgagaacctt
    18421 tgtaaacatc aaataacttg tagctttcta ctaatccttg accattttcc tttattatat
    18481 cttctatttg ttttacaaat acatcatctt taacaataag agctatatct cttgatgtag
    18541 atggatattt tggtaatggc acataattct ttgttctatc agaattatca aatacaaagt
    18601 caatatctat ttcagcgaca taaactcttt gacctagatt gtagttttct ataacatctg
    18661 gatgcaattc acctaatgta cctacatatt tattattata cactatttta gcacatctac
    18721 cagggtggaa ggtagtatta ttatcttgag gttcaatttc ataaccttta aatccgacat
    18781 ttacaagtat agtttctata gttcctttta gtgcaaagaa gtcaacatct ttaccataca
    18841 tacctacaca cattctattt tcttctttag gtaattctga atcttgtggg atgaatatat
    18901 gtccacactc aaatgcagat acttcttcta ttttatgaga tacattagtt gataaaacat
    18961 ctaacatatt tggtattaaa gtagttctca ttacagaagt ttcttctcct aatgggttaa
    19021 gtaatttaac aaagtttctc ttagcattac cttctggtac tcttatctta tcgactccct
    19081 tagggcttac aaatgaatat gttaatattt catttaatcc tacagcagtt gaattatctt
    19141 ttaaagcatc catgaatttt tgttttgaag ttttaattcc tgctgtcgta tttccttcta
    19201 attgtacaga aggtatattc tcaaatccat aaattctagc tatttcttcc catacatcag
    19261 cttcttgttc catatctgtt ctaaagcttg gtacatctat ttctaattta tcattagcta
    19321 ccaaattaca tttaaactct aatgattcta aaattcctac aaactgctcc attggtacat
    19381 ctacacctaa taggtggtta attctttgag gatttactac caatttttta ggttctggtt
    19441 tatttggata tacatctaca acacctttta aaacttttcc tgctcctaac atttcaacaa
    19501 gttgtgcagc tctatttgct gctatctctg caagattagg gtctaagtct ttttcatttc
    19561 ttgaagatgc ttctgaccta ataccaactt ttttagctgt cattcttatg ttttctggtt
    19621 tgaaattggc actttcaaaa agtacagtct tcgtattaga agttatttct gagttagcac
    19681 cacccattac accagctaaa ccaagtgatt tttctccatt tgttataact agcatatctt
    19741 tatctaatgt tctctcaaca tcatctaatg ttacaagttt ctctccatct ttagcatttc
    19801 ttactactat tcttccagtc tctacttgat ttatatcaaa agcatgaagt ggttgaccaa
    19861 gctctaacat tacgaagttt gttatatcga ctatgttact tataggtctt actcctgctt
    19921 ctgtaagtct tctttgcatc caatatggag aaggttctat ttttacatct gtaaccattc
    19981 tagcaacata tcttctacat aaatctggat tatctatctc aactttgaaa tctatctctt
    20041 cgtcactttc ttttacttcg atttcaggat attttacttt tgttcctata gttgcagctg
    20101 cttctctagc tatacccatc atgcatttac aatcaggtct gtttgaagtt aattcaaaat
    20161 ctattaaagc atcttttaat cctaaaacat cttttatatc ttttcctaat tcataagaat
    20221 cttcgtggtc taaaatatat ataccacctc ttttatactc ctcaatgtaa tgttcatcta
    20281 tacctagttc agctgctgaa cacatcatac catctgataa ttcacctctg aaatcagtct
    20341 gtttgatttc aactcctcca ggtaacttag aaccatttac agctactgga atataatctc
    20401 cttctgatat atttgtagct cctgtaacta tttgaacaac tttatctcca atatctactt
    20461 tagttacaac caacttatca gcatttggat gttgttttat ttctaaaatc tttccaacca
    20521 atatattttc tatttcttca ccataataat ctattgtttc aactttagtt cctgtcattg
    20581 tcattttatc agcgaactct tttacatcca tgtctatatc aacatagtct ctaagccatt
    20641 ttaaagatac taacatctac ataccctcct aattaaaatt gatttaagaa tctcatatca
    20701 ttttcgaata ataatctaat atcatctatt tcgtatttaa gcattgcaag tctttcaacc
    20761 ccaactccaa atgcaaatcc actgtaaact tctgggtcta ttccacaatt tctaagcaca
    20821 tttggatgaa ccatacctga acctaatatt tctatccaac cttcatattt acacattggg
    20881 caacctttac caccacattt gaaacaagta acatcaacct ctgcacttgg ttctgtaaat
    20941 gggaagttgt gaggtctaaa cttagttttg atatcagaac caaacaattt ttcaacgaag
    21001 atatccatag ttcctttaaa ttctgccata gtaacatctt ttccaacaac aagcccttct
    21061 atctgatgga acattggtga gtgtgtagca tctggcgagt ctgacctaaa acatctacct
    21121 ggtgcaatta cttttattgg taactcttga cttctcatag ttcttacttg aactggagat
    21181 gtttgagttc taagtaatac cccatcattg atatagaatg tatcgctcat atctcttgat
    21241 ggatggtctt taggagcgtt taatgcgtca aagttgtttt caacagtctc aacttctggc
    21301 ccttctgcta tagagaatcc cattccgata aatatatctg ttacttcatc tataatttga
    21361 gttattggat gcttcttccc aacctttaaa acttttcctg gttgagtaac atctatcact
    21421 tcctctttta attttctttc tttttcaata gcatttattt cttctttttt agaatttatg
    21481 ctaagttcaa tgttttctct tacctcattg gcaaccttac caactactgg tctttcttca
    21541 gcagataatt tacccatttc tttaagtatg gcagttatct cacctttttt tcctaagtac
    21601 ttaactctta aactttctac actttctatg ctttgtgctt cttttatttc agccaaagct
    21661 gcttcacgta aagcaagtaa tttttcttgc acacatatca ccctttcgtt tttttgtata
    21721 aaaaaagccc ctcaatccct gtctagggac gaaaggctat agtttcgcgg taccacccta
    21781 gtagtttaac aatttaaaaa tcatcaaacc acctctaaca aacttaacgc gtttttgacg
    21841 tcaaagccta ctgtaaaatt tcagctctgt tactccagag ggaacttctc atgacctctt
    21901 agaaaacact cccagcctag gtgtttatct ctttactaag tttaatcaaa atactttcct
    21961 ctttcattgc atttacaata ttatattgtt tattttagta ttatatcatt tccattcaaa
    22021 ttatgtgact atttttaata tatatcc
//
LOCUS       DACTGW010000047        21747 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.047, whole genome shotgun sequence.
ACCESSION   DACTGW010000047 DACTGW010000000
VERSION     DACTGW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.047"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..768
                     /locus_tag="I9573_001385"
     CDS             <1..768
                     /locus_tag="I9573_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAT4796011.1"
                     /translation="NFERDCLNSFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGI
                     ITIDIDTFKSVYCEKYLDFKRLFNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYL
                     YNDCFKIHYAVVDALIENRITAVHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQ
                     IITLNPYREDVYEFLVKEDGDFSKEIERLTEFLGYDIKEYKASLMDIYINELMESDEN
                     DVENAKEKIEKYARYIGCSDENLYVARVNAIYTFANA"
     gene            1129..2427
                     /locus_tag="I9573_001386"
     CDS             1129..2427
                     /locus_tag="I9573_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAT4796012.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSTERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKLLSSDLNN"
     gene            2617..4008
                     /locus_tag="I9573_001387"
     CDS             2617..4008
                     /locus_tag="I9573_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796013.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            4185..4772
                     /locus_tag="I9573_001388"
     CDS             4185..4772
                     /locus_tag="I9573_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT4796014.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            4805..5596
                     /locus_tag="I9573_001389"
     CDS             4805..5596
                     /locus_tag="I9573_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT4796015.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5777..8194)
                     /locus_tag="I9573_001390"
     CDS             complement(5777..8194)
                     /locus_tag="I9573_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4796016.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNLSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKTISLQGNILSIIFIPLGAI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKQAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     SYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLIRNIEKKSL
                     TERLRSQ"
     gene            complement(8207..8872)
                     /locus_tag="I9573_001391"
     CDS             complement(8207..8872)
                     /locus_tag="I9573_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796017.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGSLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(8954..9985)
                     /locus_tag="I9573_001392"
     CDS             complement(8954..9985)
                     /locus_tag="I9573_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4796018.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFNISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDLSHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            complement(9975..10661)
                     /locus_tag="I9573_001393"
     CDS             complement(9975..10661)
                     /locus_tag="I9573_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4796019.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINLFKQKVKAFLNHLTTKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(10819..11883)
                     /locus_tag="I9573_001394"
     CDS             complement(10819..11883)
                     /locus_tag="I9573_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HAT4796020.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            12145..12816
                     /locus_tag="I9573_001395"
     CDS             12145..12816
                     /locus_tag="I9573_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4796021.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKACLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            12818..13726
                     /locus_tag="I9573_001396"
     CDS             12818..13726
                     /locus_tag="I9573_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4796022.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKSEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            13803..14489
                     /locus_tag="I9573_001397"
     CDS             13803..14489
                     /locus_tag="I9573_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796023.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            14486..17041
                     /locus_tag="I9573_001398"
     CDS             14486..17041
                     /locus_tag="I9573_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAT4796024.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYIVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYLQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKRGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTGEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKS"
     gene            complement(17235..18434)
                     /locus_tag="I9573_001399"
     CDS             complement(17235..18434)
                     /locus_tag="I9573_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-0--gamma-glutamyl ligase"
                     /protein_id="HAT4796025.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            18553..19440
                     /locus_tag="I9573_001400"
     CDS             18553..19440
                     /locus_tag="I9573_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT4796026.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            19630..20187
                     /locus_tag="I9573_001401"
     CDS             19630..20187
                     /locus_tag="I9573_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4796027.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(20427..21287)
                     /locus_tag="I9573_001402"
     CDS             complement(20427..21287)
                     /locus_tag="I9573_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAT4796028.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
BASE COUNT         8130 a         2707 c         3152 g         7758 t
ORIGIN      
        1 aactttgaga gagattgttt aaatagtttt caaaatctaa agcaaatatc tgatagaggt
       61 ttaagtttag gagaaaaata tatagaagag tcttttaaga aaggaattga gactatagta
      121 agttttggaa ttataactat agatatcgat acctttaaaa gcgtttattg tgaaaaatat
      181 ttagatttta agagactttt taataacttg aataaaactt ttattatacc aaataaaaat
      241 aaaaaaaatg ggcacgctag cttcttagaa atagaatcga caataaagaa aatgagcaat
      301 tatttataca atgattgttt taaaattcat tatgctgttg tagatgccct tatagaaaat
      361 cgtataactg cggtacattc ttatattgat gaagaatcta taaaaaaatc taatgcctta
      421 tttaataatt ataaagatgg ttttattggg aaggctgacg aatgcaaagt agtaaaacaa
      481 ataataactt taaatccata tagggaagat gtatacgaat ttttggtaaa agaagatgga
      541 gattttagta aggaaattga gagattgact gaatttttag gctatgatat aaaagaatat
      601 aaagcgtcat taatggatat ttatataaat gagttaatgg agagcgatga aaatgatgtt
      661 gaaaatgcaa aagaaaagat tgaaaaatat gctagatata ttggatgtag tgatgagaat
      721 ttatatgtag caagagttaa tgccatatat acttttgcaa atgcataatt ttattaaaca
      781 ttaaatatta gatgttcaat tatataaatt taaataaaaa aatatcctta ttaataacaa
      841 atagtttata ttaaaaacca ttcttaaaat gagtggtttt ttattttgaa tagatatatt
      901 ttaaacctaa tagcattcct atctttaatt agccaaaaat caactggatt tcatatagac
      961 tacataattt taaattacta aaaaagacaa tatattgaca aaaatgaaat atgttgataa
     1021 aatgaaatag tcaatatggt gtcttaattt aaaaatttat ttatgttgta ttagataaag
     1081 ttgttttata tataaagcat aaataattat tataaggaga aatagattat gaaagtaaat
     1141 gtatacaatg tagaatcatt tgtggatgaa agacttaaga ttatggtttc agacttaatt
     1201 ccaaatacta aacttaaaat aagtgtgaaa atggaatttc catggtgcaa gggagaagaa
     1261 ttttcatctt atggagtgtt ttgttcaaat gaaaaaggag aagtagattt agatttagtt
     1321 gaacctatag aaggtactta taaggctgat aattgtatgg ggttaattta ttcgctggaa
     1381 aaatctaaaa ctgaaggtaa aaattttgca gaaaatatat ctattgataa accaataatt
     1441 atgaatatga tatttgaaac ttctactgaa agaaaagaag ttgaattaaa gagaatattt
     1501 aaatcagaag atattaccac aaaatacata tctgatgagt ttataggaaa attattttat
     1561 aaacaaaatt ctaataataa aacaatttta atgctaggtg gttctgatgg aaatttagac
     1621 gctctagacc ttttagcagc tccacttgca tctagaggat tcaatgtact aacagttgca
     1681 tactttgctt tagaagggtt accagataaa ttagaagagg ttccattaga atattttgaa
     1741 aaagtatttg agtggattag tgaaaatgaa attaccagta ctaaagaaat atttgtacat
     1801 gggacatcaa agggtggaga attggccttt ttacttgctt caagatatga acaaattaaa
     1861 aaggttgtag tatcacaacc tcacatttat tgtttccaag ctcttaatgg attgatgagt
     1921 ggaaatgata cttcatcatg gtcatataaa ggagagccat ttccatatat caaagtagac
     1981 aatgatatat tctttgaaga acagaaaaaa aatatatcaa aaggtattcc atttggattt
     2041 aatagtactt ataagaaaag tcttaaaaga gcagagaata gaaaagaggc tagaataaaa
     2101 atagaaaatt ccaaagcaga tatacttatg attgctggaa aagaagataa catatggaat
     2161 agttatgaag cttgtttaga ggcattagaa atctttgaag aaaataatta tccatataac
     2221 gtagaattat taacatacaa taatatgggt catccgttac caattccata tattatgcct
     2281 ctaaaggaga ctttatgtat gagtatgagt ggagggattt tttcttcagg tggaactgta
     2341 gaaggaaatt caaaaggaca gtttgaatca tggaatagaa ctattgaatt ttataaaaaa
     2401 cttttatcaa gtgatttaaa taactaatat taagtgattt aagtaactaa aaatgaagcg
     2461 gtttttctat atatgcacaa aatctatcaa ttttatagat ttaatttatt gtttatataa
     2521 tttgcatgtt atatactaat tttaatgaca ttgacaattt ataaacaatt ataaagttgt
     2581 aaacttataa gagttattga aaggtggagt gaagttatgg aaagactcgc tagaagggaa
     2641 caacctcata taaaatttgg aatgtttaag ataagaattc catttataca ttacagattt
     2701 gagaaaccag aagctattca aggtataata tcaagtatga caagtttagg cacaattggc
     2761 cttagtacac aaattttggg attaccctat gaagtggcat ggagtatggc tattattaac
     2821 tcaatattat actgtttgca tgtttttatg ggagacccag ttgtacctgg ctggattaca
     2881 gcttctataa ctcttacaac tgcatatttg ttaaagttca gcatgggcat tgaaagaata
     2941 caagcattaa cagctttaca aatagattta ggaataatat ttattttaat gggtataact
     3001 ggtgtagctg gaaagttagt tagtaagata ccaaattcta ttaaaggtgg aatactaatg
     3061 ggtgtcggta tatcgactat aatatcagag tttaatccta aagggagatt tgattcttat
     3121 cctatatcta taacagtagg aattttggtt gcttgttttg taatgttttc tgaaagattt
     3181 gatactctaa aagttaagaa taaattttta tttcatttag gagaatatgg tgtagtatca
     3241 gctattttag tgagtttaat agtaggtata tttagtaaag aaatagaaat tcctagattt
     3301 agttttgaca atttagttta catagtagat tttaaaaatc tgataaatac agtatctcct
     3361 tttggaatag gttttccaag tgtaatgtta tttatacaag ggataccaat ggcttttatg
     3421 atatacataa ttgcctttgg tgattttata acaggtgaaa acttagtact ttctgaaagt
     3481 gaaaatagaa aagatgaata tatagatttc aattcaaata ggtctaatgt tataagtggg
     3541 ataagaaata tttttatggc aatcttttct ccatatatac caatgtgcgg tccattagct
     3601 gcgacactta caggaagtgt tgctcaaagg tacaaggttg gaaaggaagc catgcagtct
     3661 gtttttagtg gcatgggtac attagtatgg gtttcagcaa tttttatctg tttttatcca
     3721 attgcgcaga ttgctacacc tcttatacca ttagcattgt cagtaacact tttagttcaa
     3781 ggatatttat gtacaaagct atcaatggag ctatgtaata caggtgttga gaagggattg
     3841 attggactta tgggaggtgt aattgctgct aaaggcggaa catggggact tgctgtaggt
     3901 tttatactgt atttcatttt aattgattct aaaaaaagaa aagaaaaaga tattaattca
     3961 atagaagatt gtgtatatga aatattagac aaaaagaaga gtatttaaag gaaaatatga
     4021 gttgctgtta tctattaata gattaattgc aactcatgtt ttatttataa gttttaggaa
     4081 ttgcaaaaaa atgcaaagct ttttcttgac ataatttctt gatatgttac tatagttata
     4141 taacatatgt gttttataaa aatttaatgg agaggtgaac atatatgcca cctaaagtaa
     4201 agataacaaa agaaatgatt ttgaatacag tcttggatat tacaagagaa gtgggatttg
     4261 aagctgtaaa tgcgaggagt attgcaagta aattacagtg ttccactcga ccaattttta
     4321 cttgttacga aaacatgaat gaattaaaaa atgaatttct tgcttttgca tatgaatatt
     4381 ataatcagta tgttgttaat tatcgtaatt ctaaaagtgt aagcccttat ttactgcttc
     4441 cgctttcata cattgaattt gctcaagaag aaacgtattt atttaagttg ttatttataa
     4501 atgatttaga tttgatgatg aaagaggcga atgattttta taaagaaatt gacaatgaaa
     4561 aaaaggcaag acttttttcg gaaagcattg gaatagagtt ggaatgtgca aaagtaatat
     4621 ttttagattt atttctttat actcatggca tagctgtctt aacagcaaca aaaaaattaa
     4681 cattagatag atatagtgct gaaaaaatgt tgaaaaatat attaacagct tttataagac
     4741 aagaaaaacc agattggaat ttgtctattt gatgtttatt aactaatata gaagaggtga
     4801 gattatggaa acaaaacaat acttaattga cttttataat acttatgatg aagatagtcg
     4861 acttgcatta aaacatggaa tggtggaatt tctcactact atgcattaca ttgataagta
     4921 cataaaatca ggtgattgtg tgcttgaaat tggcgctgca actggtcgtt attctcacac
     4981 actggcacgt cagggatatg atgttgacgc agtggaatta gtcgaacata atatagaggt
     5041 tttccataaa aatactcaat caaatgaaaa tataagcatt actcagggta atgctatgga
     5101 tttatctgtt ttcccagata ataaatatga tattacgtta ctgttaggac cactatatca
     5161 tctttataac aaagaagata aacaacaagc tttacatgag gcaattcgtg taacaaaacc
     5221 tggtggtgtg gtttttgctg catacgttat atctgatggc tgtctcattg acgaggggtt
     5281 ccatagagga aatattaatg tatctgaata tattgaaaaa ggtttgattg atccacagac
     5341 atttgctgct aagtctgaac caaaggattt gtttgagctt gtacgtaaag aaaacattga
     5401 tgatttgatg tctgcattta atgtaactag actgcattat gtcgcatcag atggtttggc
     5461 tttgtatatg cgtgaagcag tagactctat ggatgatgat gcatttgcgt tatatttaaa
     5521 atatcacctt gctacttgtg aacgtgaaga tttagtagga gttacaagtc atgcaattga
     5581 catatttaga aaataaggaa tatgcttgtt tatgtctctg gtgatgttgc ttggagatat
     5641 aaggaagtgt atctgtataa aataaaaaca agtagaggga cagaaaatta tgtaaagtca
     5701 aaattttcta tccttttagc ttttaaaaaa ttatgttaaa acttgtataa aaaatggtta
     5761 ttttttatat acattcttac tgagaacgta agcgttctgt aagtgatttt ttttctatat
     5821 ttcttataag taattcagtc actaaaactt gtataccaat taatacgaat gaaaaaatta
     5881 aaatagtaaa tatagaaaat ttaaaattaa taaagtctaa tctaattaca tcttttataa
     5941 aagtgcaaag tagatttgaa ccaaaataac ctagtatact tgaagtacat atagctttga
     6001 tacttatgtt tagaccttct ctataaagca tatttcgcag ttgctttctt gtcattccaa
     6061 cggcttgaag taaggcaaac tctttttttc taattaaaac gctgtttatt agggtattgg
     6121 tcatatttag tataccaaag caagaaatta aaccaacaaa aatgtatgaa gctataatta
     6181 tatattgaaa tgctctagta atcattctat aatcatcttc atatgaataa agcattaaat
     6241 ttgaattttt ctgaattata cttaacaact tttcttcaac ttctttagaa tgctttttat
     6301 ctattaaaat agaaaaatct aaatttgtat tatatggtgt tagagattct aaagttttgt
     6361 ctgaagcaaa aaacatacct cctgtattgg aattgtcaat gattcctata attttcattt
     6421 tctttttaat aatatcattt ccactaaaga tattaaatgt aactgtatct ccaactttta
     6481 aatttaatcc ataagatttt gttctatatt tatttataat taaactatct gaatctaaat
     6541 gtgtatagtc tatttttcca ctgacaatat attcttcaag tgattttgaa agttcaggcg
     6601 taataccttt aattagagta ataaattgtt tttcgtctct atctttgatg taaggttcta
     6661 taatattact tttaattgat ttggattcaa ttaatttttt tactccatga atagatttaa
     6721 tttcattctt aagagaatct gttagtggat tattttgttg gatttctgta atatgctcat
     6781 ataagttatc actcttaatg ctaattatat aatcttcgtt atagacaaat tgttgagata
     6841 gttgtttttt gaaatctatt gaattagcaa gattagcgat tgaaataaat aaaactccac
     6901 taatgcttag cgatattaat gcaatcaaat ttttctttct atttgactcg atattcttta
     6961 atgctagatt ttttatattt atataactaa aattttttgc ttgttttcta aatttagttt
     7021 ctgtatattg catggcttca atagcagata ttttagaaat aattctagct ggtttaaata
     7081 aagaaattct aacagttaaa aacattacca gtgataaaat taaaattata aataaatcag
     7141 ctatcatttg ccatgctaat ggttgaatta tgtaagcaat tatacaagaa gctatagccc
     7201 ctaatggaat aaaaataatt gacaaaatat ttccttgtag agaaatagtc ttttttaact
     7261 gaaaagaagt catacctaac gatttcaact tagcatacat ctgaactgaa ttaacaactg
     7321 aaatataaaa aatactatat ataacaaaaa cgctagaaaa aagaacaact attccaacta
     7381 ctaaaactgt aataatctgc tcaccatcaa ttaaatttga ttcaatatag ttatcattta
     7441 agtatatatt atatttctta atacctaaag ataaaccaat tttctttaac tgtgtcttaa
     7501 ttgaagctga atctttctct gtattttctt ctgatagttt aactaataca tctacagtat
     7561 caggtcttga ctcgtcaaaa gtattacttt tattttgtaa aggtgtagaa gctatattct
     7621 taaaataatc atttgaaacg actgtatagt attgcttggc attatcttga taataatttt
     7681 ctagaaatcc aacaataata aaatcacatt gaacttcttt attcgtaata tcatcataat
     7741 aatttaattt aatgctatct ccaatatctt tatctaaacc aaattcctgt aaatattttt
     7801 ctgaaatagc aatttcattt ttatttgtag gtagtttgcc tttcaatggt tgaaaattca
     7861 taagtttaat tgctgtatca tctgcataaa caatcgacat aatacagtct gtctttttat
     7921 tacttccaat taatttgtag gttcctacgc cactaaggtt tttatcttta tatagtatat
     7981 tttttgcttt ttcatctaca gatttatata tagcatgata tggagatgca ttaactaaat
     8041 ctacagaagc attatatgaa actaaggtca ctgttaataa taaacatgta gaaatcatta
     8101 taatgatacc tgaaagatat gttttaagtt tattaatttt taaatcattt aaagctattt
     8161 ttcttataaa tgtgcgatta ttatttctaa acatatacta cacctcctag ttaacaattt
     8221 ttccatcttc aatacgaata attctatcag ttagttgtgc aatttcatta ttgtgtgtaa
     8281 tcattacaat tgtttggttg aattttttac ttgtcacttt aagtaaactc agtacatctg
     8341 aactagttct actatcaagg ctacctgtgg gttcatcagc tagtataata gctggttttg
     8401 ttacaagtgc tctagctata gcaacacgtt gctgttgacc accagaaaga ttacttggca
     8461 tatcatataa tttttcttca agagaaagca tatttataat ttcatttata aaatccttat
     8521 ctatggtctc tccatccaat cctattggta agataatatt ttcatataca ttaagtaaag
     8581 gaacaagatt ataattttga aatataaaac ctatatttct gcgtctaaag attgtaagct
     8641 gttcatcagt catagtagat aattccttac cctttataat aacctttcca gatgtaggat
     8701 tatctaatcc tcccatcata tttaataatg tggatttgcc actaccagat gtacctacaa
     8761 tagaaataaa ttcgcctttt tcaatagata gagttacacc atcaagagct tttgtaatgt
     8821 ttgaactttg tccataatat ttctttaaat taattacttg taaaatactc ataataatac
     8881 ctccttaaca tcttgtaaat acagtataaa atcaaaatgt tgcagaatca ttacaaataa
     8941 aatatttaaa aatttattta ttaggtaaga agatagaaaa agtcgaccct tttccaatat
     9001 ctgatgtaac tttaatatag ccaccttgaa gagatataat ttttcttgaa agatatagac
     9061 ctattccaat accttctatg tcatgtacat cttcttcacg ataaaatctt ttaaagattt
     9121 ctgcttgatg actttctgat attccttttc cattatcaat aatatctatt tttgtataca
     9181 taacccaaca ttctgtagct acacgtatct taccattaga atttgtgtat tttacagcat
     9241 tatctaatat attaaaaata gcttcagaag tccatttctt gtcatgaatt atatataaat
     9301 ttgggtcaca atcaactgta acttcaatat tctttttttc agcatcaaac aaaattccac
     9361 taagtgctgt agcaattgtt tcataaattg aatttttctt tttagatagt gtgattatac
     9421 ctgtctctag tctggaggtt tttaccatag attgcattaa aaaatctaat ttatctaatt
     9481 ggttttccat atccattaaa aaatcctttt gaatttctgt tttaaatgtt tgatttagta
     9541 gagtagtatt aatcattttt aaatttgcaa taggagtttt aatttgatga gatagatcag
     9601 aaactagttc ttgtaaatca gctttctcta tagcaatact attgcgattt tcttgaataa
     9661 cttcatacat tctaattaat ctgtgattga tacgtgataa aatagtttct tcttctaatt
     9721 caatttcaat attgtcttca ctattcatca tactatctaa agtgtgacat attttagaac
     9781 aaaatataac tattttttta tgaagtaaaa atattattaa tgctataaat ccacaaagta
     9841 ctattgtata tattaataat agagcaatta gttttaaatc atcagttatt agataaattg
     9901 aaatattaaa taaaatagct gtaaaaatta taccaacagc aattattaaa taaatagaac
     9961 taaaagaaaa ttttttaaaa ttcatttctt tcacctaacc acatatatcc cataccatat
    10021 accgttttga tatacttaag attgtctttt tcaattttgc ttctaatcct acttataaca
    10081 gatgttaaag tatgttcatc tacaaagtta gcatctatat cccagagttt ttctaaaagt
    10141 actttccttg ttaaaatact tttaggattt tcagtaagca gttttaaagt acgatattct
    10201 aatggtgtaa atataatttg atttccattt atattggcag ataactcaga aaagttaatt
    10261 tcaagatatc catccctata gtaatcttgt tttgtttttg ttgtaagatg atttaaaaaa
    10321 gctttaactt tctgtttaaa taaattgata ttaaatggtt ttgttatata atccaaagct
    10381 ccaagttcat agcctttaat catatcacat tccatatcat tagcagttaa aaaaattatt
    10441 ggtatattgt aattttcctt tatttcatta cataattcaa acccactttt atctggtaaa
    10501 ttaatatcca aaattattaa atcaaattca cattcaaaaa tagactttag tgcagattta
    10561 gctgtaaatt ttgaagtaat tatatatcca tcttcaatta ggttatatga tagagtttta
    10621 tttaatatag aatcatcttc tactactaat attttttcca tacagttact ccttttagtt
    10681 taatatttta taatgtcaat tatagactaa aaacattgct gaattgttat aaacgagaaa
    10741 aagtatttaa aaatttatat atttaataaa gaacctgtcc tatgataaat tctagtacag
    10801 attctttatt aaataaaatt atttcaacca agatggattt ccaggtataa atattctttt
    10861 accaaatttt gaagtatact catctaaagc tttttgaata agctcattat tacccttaaa
    10921 tgaagtagta ttctttatag tttcgagatt ttggctaaat gggcaagcag atatacacat
    10981 accacaatct gtacctaaat atctccattt aatatagcat gtctcatgtt caattaccca
    11041 attatatttt ccatcatcac caattttaat atcattagaa agcgaatgag aagggcagtt
    11101 aaaagagcat tttttacaga ccttacaaaa atcttctagt ccaaaatcaa catattcatc
    11161 ttctaaaaga ggaatatctg tagtaacaac acctaatcta agtctagagc cataatcttt
    11221 atttgtaagt atagcatttc ttcctatatc tcctaaacct gcatcacgag ctataaaaac
    11281 aggcattact aaataattag catccatatg atttctagca tcatatccta aatttcttat
    11341 atagtaactt aaaatcatgc cgacgataga tgcatccaca tagcactttg atgtttctat
    11401 aacttcacat atcattggag ctctatttat catttcttta tccatctcac atccaaatac
    11461 aattgcatac ttatgagtta gattaatttc ttcaccgtaa ttttcttcat gtctacccct
    11521 atttgtataa atatgatagt cttttaattt agttatacca actacagaag ctccatactg
    11581 ctttgcaaga cccttaattt tttttgtcat tgactttgca tctacatcta ctttagtatc
    11641 agaaactttt ccttcgcata aagatttgat atcagataaa aaatcgaatg cactacttgc
    11701 cattggagag tttaactcat tgtaagtcat tgttccttca ctacataaat ttggtctatt
    11761 tcttatacta tcatcaattt cttttttgtc aggatttttc ttataataat cattataagc
    11821 agtactatct tttttgtaat ttgctcttgc aaacatagta tttctctcat caaccctttt
    11881 caattctatt ccccctagta aaaatatatt taaaaaatat aactttattg ttgtataatt
    11941 taattatata agaataacgt tatattttca atttaagcgt aattaaattg ggaaatatat
    12001 aactatatgc ttgattatga tgtttttatt ttgaaaacaa atttaaatac aatcttttta
    12061 gtgaaataaa taaaattaaa atatttttag tatgataatg caagaaaata attatttatc
    12121 taaaaaatct aaaggagagt aaaattgaac ttattaatta ttgaagatga tataaactta
    12181 aatgaaggtc ttttttatgc atttgagaat gatggattta atgtatttaa ggcttataca
    12241 aaacaagaag gattaaatat atttaatagt aaaaatatag attttataat cttagactgc
    12301 aatcttccag atggagatgg atttgatgta tgtcaaataa taagggaaaa gtcagatata
    12361 ccaattataa tgcttactgc aagagactca gaaattgatg aagtaaaagg attagaaata
    12421 gggcttgatg attatattac aaaaccattt tctttatctg tattaaaggc aagagtcaaa
    12481 gtagcaataa gaaaaaaatc gaataataaa gtaatatatt ccaatggaat aaaattggac
    12541 caaaagttac taaaagtata taaaaataaa gcatgtttgg aacttagctc tgtagaatat
    12601 aagcttctaa gttatctaat tgaaaataaa ggtcagatat tattaaagga acaaatttta
    12661 caccatattt gggacagcga agaaaattat gttgatgata atattgtatc tgtaaatata
    12721 agaagactta gagtaaaagt agaggatgac ccatctaatc caaaatatat aaaaacagct
    12781 tatggaatgg gttatttatg gaatgaggta gaataatatg tttatattag cctttataat
    12841 atgtacagtt gttatattta tagtatctac taaaataaat caaagaagat atgatgaatt
    12901 aaatttgatg ttaaatcaaa tattggaagg taaagaagtt acttatccag atacgaagga
    12961 tacaagagcg tcaaaaattt ctcaccaagt aaaaaaaatt aaggatatga tagaaataga
    13021 ggtagaacag tcaaagtcag aaaaagaagc gattaaaggt ttaatatcaa atatgtcaca
    13081 tcaattaaaa actccacttt caaatataac catttattgt gaacttctgg agaatataaa
    13141 tatttccaca ttacagaaga aggagttttt acaaaagatg aaaaatgaaa cttttaagat
    13201 tgactggttg ttacaatcct tatttaaaat gactaaatta gaggatggag ttatagaatt
    13261 tgaagtagaa gaactactaa ttaaagatac tcttattcaa agcatcagta ctatctttaa
    13321 taaatcagaa gctaaaaata taaggataaa tctcgaacca ttttcagata taaagttagt
    13381 tcataataaa aagtggacca tagaagcaat tgtaaatgtt ttagaaaatg caataaaata
    13441 ctcaccatca gatagcacga ttactatttc tgtaataaaa atggagctat atacgaagat
    13501 tactattaaa gatgaaggaa taggtattga tagtagagaa ctaaatgata tttttaaaag
    13561 attttacaga agtaaaaatg tggaaaacca aaatggcaca gggattggtc tatatcttac
    13621 aagattaatt ttagaaaaag aaaatggaaa cattatagta gaatctaaac taggaagtgg
    13681 atgttgtttt agtatattct tacagaattg taagagttta aactaacttt gtaggatgag
    13741 tgtaattatc gtcacttaca atattttata gaacatatat ctgtattgaa aggagaaatt
    13801 aaatgagtat attagtgacg aataatttag tgaaacacta tggaaaaggt gagactaaag
    13861 taaaagcctt aaatggagta agtatagaaa ttgaacgaga tacatttact gctatagttg
    13921 gaacttctgg ttctggaaaa agcacattgt taaatattat aggtggatta gataatccaa
    13981 cctcaggtga tgtaataatt aaaggaaaga acatatctaa aataggaaaa aaagatttga
    14041 cagtatttag aagaagaaat ataggattta tatttcaaaa ctacagttta atgcccgtat
    14101 taaacactta tgataatata gctcttcctg ttacattaga taaaggaaac catatagacc
    14161 atgaatatat tgaaatgctg atgaagacat tgggaatttg ggataagcgt ttaaaatttc
    14221 caagcgaact atctggtggt caacaacaga gggtagcaat tgctagagct ttggcaaata
    14281 aacctgcact tatattagca gatgaaccaa caggaaatct cgatagtaaa actacaatgg
    14341 aagtggtatg tttattaaaa gaaagtagtg caaaattcca tcaaacgata cttatggtaa
    14401 cacacaatga aaatatagca caagtatgcg actcaattat ccatattgaa gatggagttg
    14461 tggtgaatac tggtggtgaa gtactatgat gtctctagta aaattttcaa ttcaatatat
    14521 aaagcattat aaaaaacaat ctatatctat agttttaagc atagtactat cagtagcatt
    14581 attgacggga attggttctt tagttcatag tgcagacaaa agtagaattg aaaagataag
    14641 ggaagatagt ggagattatc attatttcta taaagtagat aaggaacaac ttaataaaat
    14701 taaaaaaaat aaaaaatcaa aggaatacat tgtagataga cttggcgtta caaaacctaa
    14761 aaatcagata gatgaacctt ttattataga atttttaaat gctgattctt catatttaga
    14821 catgacaggt agaaaattat taaaaggaac tcttcctagt aaagatggag agatagtact
    14881 tgacacatat tctctaaaca atttaaatgt aaacaaagaa attggaacag aaatcaattt
    14941 agatggtaaa atctataagt tatgtggtat tttatcaagt ctttcaaacc cagaaactgc
    15001 tatggaggga tttgtaaacg attatttgca gcctaaagaa aaggattctt atatagttta
    15061 tgtaaaatat gatgaaaata aaaatattaa aaagcaaaat tataaattta tgaaaaaatt
    15121 caacatatca aaagaaaaaa cttatataaa ctggcctctt tcagttgaat ttggagtaaa
    15181 accaccaaag aatatagaaa aatataatat atttgagttc ttaaaaaatt tagaactaaa
    15241 tacaaatgca attatattgc ttgttggagt gtttggtgca gcagcagtat atagtatttt
    15301 ccatgtttca atacttcaga gaatatcaca atatggggtt ttagaagtat taggcgctaa
    15361 taataaacaa cttttatttc tactattttt agagttattt ttacttttta ttgttggatt
    15421 tcctataggt tgcttcttag gtattggagt agcgtctaca atatatgaac aatttccaca
    15481 tatatttttg agtagtgata ttgttccagg agcatttttt ataagtgaaa aatctattta
    15541 tttaggcttt ttatttatct tattgttgtt aattttaatt gctgtacgtg ttgtacatca
    15601 gttgaataaa tactctagta tagaatctat gaaaaacttt aagattacta ataagaaaaa
    15661 gagaatatta gaaagtgtaa aacactctaa catgacaaaa gtattatctc ataaatacat
    15721 gactcaaaaa aggggaatgt ttattggaat tttgttttca ttatcacttg gtggaattat
    15781 atttctatgt tctagttact ctatacagct tactaaaaac aataatgaac ttacaatgaa
    15841 agctgatgat ggtttaaatt ctgactacca aataactatg caaacaacag attttgatgt
    15901 aggtatacca aaagataaaa tttcaagatt aaacaatgtt gagggaatat caaaagtcta
    15961 tccagtaaga tatttatttg gagctatgca aattaaagaa gaacaactat tttggaaaaa
    16021 tttctttaaa cctcttgaaa aagattacag gataaagact ttttttgatg gtatttgtac
    16081 aaagcaaaat gaaggtggct atcttttaaa aacaaatatt tatggttata acaatggtat
    16141 gttagaagga ttaaattctt atattataga tggggagatt aatacccatg atatggttaa
    16201 aaataataag gttatagtta gactacctat ggatggaact gggatgtatg atgcagttga
    16261 tataaatcca ggagatacaa taaaagttaa agtgccaaag acaatgaaac caacaggtga
    16321 aattgttaaa tttaaagaag aaaataagaa tgattataca acaaaagaat ttgtagttgc
    16381 tgctactgta aaacgtgtga tggcaaataa catatatttc attggtgatt atggtatgga
    16441 tatagttatg acgaatgggc aaatgaaaaa taactttgat atcgaaaatt ataattcagt
    16501 aagtataaaa aagacagaag atagtaacag tgaggaattg tctgagaaca taaaaagtgt
    16561 tgtaagtgat gtaaaaagat gtatcgtaac agattatacc attgctattg aaaaaaataa
    16621 tacctactta aatcaaaaac tcttatttat atatggtatt gtatttgtac ttcttgcaat
    16681 aagtttgttc catataatca atacagtaag ctacttaatt ttttcaagaa gacatgagtt
    16741 tggaatttta agagctatgg gtattacgga taataagttt ttacttatga tgataagaga
    16801 aggtttttta tatggattct atgcaagtat tataatggta attggaagtg ttattggaca
    16861 atttatgatt tattttatgg taaaacgtgt atatttatat ataaatccaa ttcttaaaat
    16921 aaatacacct ctatacatag gtatgattat tttaaatatc actattagta tagttgctgt
    16981 aattattcca gtaagacaaa ttttgaagag tgatattatt tcagaaataa ataagagtta
    17041 gagttatata aatttttaag attatataac tttatgacaa tcttgtttaa attatttaaa
    17101 tatatcttat ctgtgaacat taaataaacc tcttaataga tataactctt gaaaagtcat
    17161 atctattaag aggttttaat aaattttcat gttaatatat ataatccaaa aaaattaaat
    17221 cttattaaaa catactattt tgtatagtta tcgaaatatc cttgtatgta aataatagga
    17281 gtacccttat ctccacttcc agatgttaaa tctgctaaag aacctataag gtctgttaat
    17341 cttcttggag tagtaccttg agaaaccatg tttcctgtta aatcatcaga tttattatct
    17401 ttttcaacta tgtattttga aattgcttct tttaactcat caccagataa gtcagcaaaa
    17461 tcattatctg ctaagtattt taattttact tcgtttggag ttccctctag cccttttgta
    17521 taagcaggag atactactgg gtcagctaat tcccatattt tacctacagg gtctttaaaa
    17581 gctccatctc cataaaccat tacttctaca tctttccctg ttatttcttt tatattacct
    17641 tggattttgt ttacaacttc atcgcaattt atagggaata attttactgt ttcttctgta
    17701 gctttgtttg aacctaaaag tccatactgg tcattgtaac cactaccatc tacacttgct
    17761 gtcattatat catcaagtga atatactttt ttagctccat tttgtcttaa aattctcttt
    17821 gttctttgtc ttgtatgtat atcacaagtt aaaatacttg ttgtatagtt aagaatagtt
    17881 tttggattgt ttgcaaatac tatttctacc tctgcaccac aatccttgat taaattctta
    17941 taataatcaa cataatctac accagtaaat gtatgcttgt tatatccaaa tagttctctg
    18001 tatttttctt ctgttaaaac atctgaccaa ggatttattc ccttatcatc aagttcatca
    18061 atacttatta agtgatttcc aacttcatca gaagggtagc ttaacattaa tacgactttt
    18121 ctacatcctt ttgcaattcc ttttaaacat atagcaaaac gatttctact taaaattggg
    18181 aatataaccc caacagtatc atctccaaat ttatctttta catcttttgc aatattgtca
    18241 acgtgagcat aatttccctg tgctctagct acaacagctt ctgtaacggc aattacatct
    18301 ttgtccctaa cttcaaagtt ttcacttttt gacgcattta aaacagaatc aacaactatt
    18361 ttaactaagt catctccttg tcttataata ggtgctctaa cccctcttga aacagttcca
    18421 actaatctat ccattatcta aaatcctcct aaagaattaa tattgtcata ttagtaccat
    18481 gtatattata attcaaatat aattataagt aaaataaata atactaatat ttgacataag
    18541 gagtaattat atatgaatat aaaacttgaa ctttataagg tttttaatgc agttgtaaat
    18601 aataagagtt tttctcttgc tgctaaggaa ttgtttatgt cacagccagc tgtaagccag
    18661 tcaataaagc aattagaaga acaattagat actttattat tttatagaaa caataaggga
    18721 gtaaaattaa ctccagaggg gaaaattcta agtgaacatg tcactactgc attaaagttg
    18781 atatcatcag gagaagatag aataaataaa tttaaaaaat tagaatacgg ttctctgaaa
    18841 ataggagtag gagatacagc tgcacgtttt ttcttgttaa aatatttaga aatttttcat
    18901 aagaagtatc cacatataca tgtatctaca ataaatagaa cgagtagaga acttatatct
    18961 ttactcaaag atgggaatat agatatagca attataaaca tgccaataga agatgataca
    19021 ctaaatattg ttgagtgtat tgaaatacat gatatatttg tatgtgcaaa tgattacata
    19081 gaatacaaag gtaggaaaat atcattggaa gaattaaaca cattgccttt aataatgcta
    19141 gaaaataaag caaattcaag actttatgta aatgaatatt ttttatcaaa aggtataaaa
    19201 ctaaacccgg atatagaatt gggttctcat gaattacttt tagaatttgc gtatataaat
    19261 cttggagtgt cttgtgttat agaagaattt agcatagatt atttggaaaa tgagaaattg
    19321 tttaaattag atataaaaga gccaatacct aagagaaata taggttactg ccatttaaaa
    19381 gatatatctt tatccttagc aaccaaagaa tttttaagca tgatatctaa taacatataa
    19441 tttagaatac atatttcaca agtaatttaa tataaaaata gagataaatt cgtaaatttg
    19501 aaagattcat atagtaatta gtttttatta aaaataaaga taaggttaat ttgagaacat
    19561 ttatataatt aatatcgtat tttaaatatg aacagtatat ttagattaat gtataaaggg
    19621 ggattagaaa tgtctgattc tgaatatcct caaatgaaaa ccttagaaac tagaagttgt
    19681 atactaagac ctgcatcatt agatgatgtt gaagattttt ttgactgtta taaaaataaa
    19741 atagtagtaa aacatttacc atttagtgag cataaatctt tagaagatac aagaaagttt
    19801 ataaaatcat tttttctaaa tccctataaa aaagggaaaa ttggccactt tgctatagta
    19861 tataaaagag acaataaagt tattgggaat atggggttta ataatataaa tcctaaagca
    19921 ttagaagcag aaataggtat atgtataaat ccaagctatt ggggacatga ttttgctact
    19981 gagataacaa aaagagtaat acagtatgga tttagagaac taaacttaaa taaaataatt
    20041 gcaataacct atgaagaaaa ccaaaattct acaaaatcat tagatttatt aggatttaaa
    20101 tgtacaggaa agtatatcaa aaaaattcat actggaaata ctgtaaaaaa tgttccttgt
    20161 tatcaatatg aattgaaaaa acaatagata taatcattta atatctatcc tggttattta
    20221 gtaatgattg ttttctaaat aaaatttcaa taaagtagtt gactcaaaat atagataatc
    20281 ctgtttttta ttgttaaaat actgaaaatg catttgattt tatacaaaaa agagtgtctc
    20341 atgaactaaa aagtttatga gacactcttt ttgaataaat cgtattttaa ttaaaatagt
    20401 atatatactt aaaatattaa tttttattag ttctctaaat ttttaaatcc ttctccaaga
    20461 acctcatgca catctgtaac aataacaaat gcagtagggt cagttctttt aactaatctc
    20521 tttagataaa gttcttgttt tttagaaact acgactaata gaacttcttt attttctcta
    20581 gtgtatccac ctttaccatc aagaatagta agacctctat caagttcttc agttatagct
    20641 tgtcttaaac tttcaggctc atcagagata ataaaaaatg cttttgaatg gtcaaatcct
    20701 tccattatca tatctgctat ttttattaat acacaaagtg ctataccaga gtaaagagca
    20761 gtttctaaac tacgatttac tataccagaa gatataacaa ccattccatc taggcatgac
    20821 atgaattttg tagcttttat tcctgggaat ttcttactaa gtatgagtgc aattaagtct
    20881 gttcctccag tagaagcatc tgatttaaac attattccta aacctatacc aactaggatt
    20941 cctcctgcaa tagaagacaa taataagtca ttagtaaatc ttagttttga taatgggtct
    21001 gttaattgaa ctatagctga aaatacaact gttccaaata aagtttttaa tgaatttttt
    21061 gtacccataa ttctaaaagc aagtattact aatgggacac ctataattaa catgatagct
    21121 gatactggaa gtcctgtaac cttgtttagt actaatgata gaccactaag tcctccagga
    21181 gcaattgtat gtggttttaa aaacatattt ataccaatac tcattgatat acaacccaaa
    21241 aatagtccaa gcatttcaag taaaattggc gacttttgtt ttttcatata aacacctcca
    21301 tgaacttgtc acaatttaat attataacat atttgaccag tttatattat ataaaatttc
    21361 ttgatataag caatttcaag tatttcatta ttcttaatat ttttgaattt ttatatagtg
    21421 ttttcatatc tttactgatg ttgttacact cctatgaagt tttttatata ttataatttt
    21481 tttattcttt tataaagtat cccctataaa gatggttcta tgaaaatttt ctctatactg
    21541 tttaacgtat atagagattt tgtgtataat tataaggtat ttaatggaaa aaattatctt
    21601 gcttttatat gttatcaaaa ggatttatta aatacaacag acagcttgat aacatataat
    21661 tatataaata taatttatgt agatagttgt atttttaaat aaatttttac atatcaataa
    21721 ttattatcta ttttatataa gcaacaa
//
LOCUS       DACTGW010000048        21690 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.048, whole genome shotgun sequence.
ACCESSION   DACTGW010000048 DACTGW010000000
VERSION     DACTGW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.048"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..397)
                     /locus_tag="I9573_001403"
     CDS             complement(<1..397)
                     /locus_tag="I9573_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAT4796029.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYN"
     gene            complement(409..1218)
                     /gene="aroE"
                     /locus_tag="I9573_001404"
     CDS             complement(409..1218)
                     /gene="aroE"
                     /locus_tag="I9573_001404"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAT4796030.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRIFEILNIESAYKNFEIPKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            complement(1265..2461)
                     /gene="pheA"
                     /locus_tag="I9573_001405"
     CDS             complement(1265..2461)
                     /gene="pheA"
                     /locus_tag="I9573_001405"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAT4796031.1"
                     /translation="MEDLTRYRNEIDNIDKEIIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAIDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKTISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYELEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAENNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            complement(2464..3543)
                     /gene="aroC"
                     /locus_tag="I9573_001406"
     CDS             complement(2464..3543)
                     /gene="aroC"
                     /locus_tag="I9573_001406"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAT4796032.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDKIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQRDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            complement(3521..4834)
                     /gene="aroA"
                     /locus_tag="I9573_001407"
     CDS             complement(3521..4834)
                     /gene="aroA"
                     /locus_tag="I9573_001407"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008421733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAT4796033.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNSTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            complement(4834..5898)
                     /locus_tag="I9573_001408"
     CDS             complement(4834..5898)
                     /locus_tag="I9573_001408"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAT4796034.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            complement(5920..6933)
                     /gene="aroF"
                     /locus_tag="I9573_001409"
     CDS             complement(5920..6933)
                     /gene="aroF"
                     /locus_tag="I9573_001409"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAT4796035.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            complement(7395..8351)
                     /locus_tag="I9573_001410"
     CDS             complement(7395..8351)
                     /locus_tag="I9573_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAT4796036.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFIGIIIFKIKKLL"
     gene            complement(8690..9388)
                     /locus_tag="I9573_001411"
     CDS             complement(8690..9388)
                     /locus_tag="I9573_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4796037.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            complement(9422..12124)
                     /locus_tag="I9573_001412"
     CDS             complement(9422..12124)
                     /locus_tag="I9573_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HAT4796038.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEVITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSSKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            complement(12302..14119)
                     /gene="hflB"
                     /locus_tag="I9573_001413"
     CDS             complement(12302..14119)
                     /gene="hflB"
                     /locus_tag="I9573_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAT4796039.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(14150..14593)
                     /locus_tag="I9573_001414"
     CDS             complement(14150..14593)
                     /locus_tag="I9573_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT4796040.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(15084..15974)
                     /gene="metA"
                     /locus_tag="I9573_001415"
     CDS             complement(15084..15974)
                     /gene="metA"
                     /locus_tag="I9573_001415"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAT4796041.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(16004..17269)
                     /locus_tag="I9573_001416"
     CDS             complement(16004..17269)
                     /locus_tag="I9573_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HAT4796042.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(17379..17481)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(17535..20192)
                     /locus_tag="I9573_001417"
     CDS             complement(17535..20192)
                     /locus_tag="I9573_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAT4796043.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNFSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKRKK"
     gene            complement(20320..21063)
                     /gene="yaaA"
                     /locus_tag="I9573_001418"
     CDS             complement(20320..21063)
                     /gene="yaaA"
                     /locus_tag="I9573_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAT4796044.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIIN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            complement(21073..21537)
                     /gene="bcp"
                     /locus_tag="I9573_001419"
     CDS             complement(21073..21537)
                     /gene="bcp"
                     /locus_tag="I9573_001419"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAT4796045.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
BASE COUNT         6761 a         3897 c         2364 g         8668 t
ORIGIN      
        1 gattatacaa tctatctttt ccgtttttta ataatggtct tgaattaata tttatatctt
       61 ccaaaatctt ttgtataggt ctatcaataa aaattattat acctgtttct tttagtattt
      121 ccatattaac atcctttttt attacacctc cacctgtaga tatcactgtt ttatcactta
      181 tagaaagttc tctacatgct tgagtctcat agttcctaaa gatatcttct ccttctgaaa
      241 acaactctgc tatacttttt tgagatattt gaattatata atcatccata tcacagaaac
      301 tacaattata ttctttggcc aaaagtttac caattgtagt ttttccacta cctggcatac
      361 ctattaatat caatttttct tttgttttat ttatcatgac aatctccctt acaaaaattc
      421 taattttagt tcattatata taacatctaa aatactatta tctattttag tatcttgcca
      481 aatttcctga gattttattg cttgccctac cagcatatat aatccatcac aagttttttt
      541 acccatacta ttaccaattc ttaaaaactc tgtttctcca ggattgtaaa ttaaatctat
      601 taatatatca aaattttgta taatagattt tgacactggt gaaattccta cattagggta
      661 catacctact ggtgtagcat ttaatattat atctcctttt atatgtttaa gttcttcata
      721 atctattaat atagctttac tatttaataa ttttttatca tccttttttc tagttgaaac
      781 ataaagcttt tctataccag aatcaataaa atatgtaaga gcagctttag aagcacctcc
      841 agttcctaat ataactgcta cttttccttg aacatcaata ttagccattt taaacatact
      901 atcaagacca aagtaatctg tattgtaacc atataacata ttttctctga gtaaaattgt
      961 attaactgct cctatacgtt ttgcctctgg tgatataaaa tcaagatatt tcataatacg
     1021 ctccttatat ggtacagtaa catttactcc ttgtattcct aataatttta tagcaccgtc
     1081 tagcttagat atatcttctt ttggaatttc aaagttttta taagcacttt caatattcaa
     1141 tatctcgaat attctcttgt ggatttgagg tgaaacactg tgagagagtt tttcacctac
     1201 tagaccgaaa aaattcatac ttacacctcc taataattat aaattaaata aaaatttata
     1261 ttatctattc agttttattt ttatatactc ccataaattt aaaatatgct gtattatgtt
     1321 ctatcaattc taataagtcg tacactttag aattgtatat agaacactca aaatctatat
     1381 aaaagaaata tctccatgag gcatttttca taggtcttga ctctattttt gtcatattta
     1441 tattattttc agcaaagtat ccaagtactt tataaagttt tcctgcttca tgttcaacag
     1501 aaaatactac actcattttg tttgattctt cttcaatgtg tatttgatta ctgattacta
     1561 caaatcttgt atagttgttt gcaatatcat ttatacaagg acttattatt tctaattcat
     1621 aaatacttgc agcttttttg cttgcaattg ctgcttttgt tttatcttga agttttctta
     1681 caagttctgc acttgttgct gtgttattaa atggtatcaa tttccatgta ctattttgtt
     1741 ttaaaaattc actacattgt cctatacctt gtggatgaga atatatttct tttatatctt
     1801 caagttttgt gtcttttatc cctattaaat tttggtctat ttttatacat tcttctccaa
     1861 ctatataaaa accgtacttt ctcaaaaggt cataagtttc agagatagct cctgtagatg
     1921 aattttctat ggggattatt ccatagttta tttctttatt tttaagtgct atgaaaacat
     1981 cttcaaattc tttaaattgt ttttttgata ttgttttgtt gaagaattta tttagtgctt
     2041 cttctgtaaa tgaacctgat tttcctggaa atcctactag gatatttttt ttatttaatt
     2101 catcttttaa agacttatat ttatttacat ttgacaaatc aaatttctta tttgatgaat
     2161 ctaaactaaa ctcgattttc tgctcaccat cattatctat caatcttttt tgtaatcctc
     2221 tactaatttc cattatagaa ttaaaaaact gtaccgtttc ttgtgagtat gctttattat
     2281 ctaaattatc tatggctcta cttagtactt tttcttctct atctttttga agtatagtag
     2341 tgttattttt cattttatgt ctggcaattt caagaactgt attcattctc ttttcaaata
     2401 attgaataat ctctttatct atattgtcaa tctcatttct atatctcgtt aaatcctcca
     2461 ttattatcgt cctttcatca aatcaaaaat tccaatggct gttactgctt ctattacagg
     2521 tatagccctt tgaactatac aagggtcatg tctgccttta atatataaaa tatcgtcctt
     2581 tttatctttt atattaactg tgttttgttg tctgctaatt gaaggagttg gcttaattgc
     2641 cactttgaaa attattggca ttccagtagt aatccctcct ataatccctc cattgttatt
     2701 tgttttgctc ttaacttgat ttccttcata atacatttca tcattacttt gagagccata
     2761 catatctgca agttcaaaac ctaaaccaaa ctctacacct tttacggcag gaacagaaaa
     2821 caataaatgt gataaagtag attcaacaga gtcaaaaaat ggatttccaa gccctacatt
     2881 aatacctact actgcacact caattatacc accaactgaa tctctttgat tttttgcatc
     2941 tataatcgta tttttcatgg cttcttcctt tgataaatca agtaaaggta gttccaaagt
     3001 ttgcaaattt gataactgtt gttttgaaat atttacataa tcaaaactca tatcttctat
     3061 atttttaatt ttttttatgt gtgctcctat ctctattcct ttttgtgata aaatttgttt
     3121 acatatagcc ccacaaaata ctaatggagc tgttatccta cctgaaaaat gacctccccc
     3181 tctatagtca ttaaatccag aatatctcac attacctgtg aaatctgcat gtcctggtct
     3241 cattaaattt ttaagctctc cataatcttt tgaacgagta tcactatttc ttatgattgc
     3301 acataatggc gttcctgtag ttcttccatt aaaatatcca cttagaattt ctggtatatc
     3361 actttcattt cttgaagtag atatagaatt ttttccaggt gctcttctct tcatttcttt
     3421 atcaatctta tcaaggtcaa gttcaatccc cgaaggaaga ccatctatat ttatacctat
     3481 agcatttcca tgagattctc caaaaattga tacttttaaa ttatttcccc atattccact
     3541 catcaattcg acctcctaac atcttaaaat ctttaaaaaa atgtggataa gattttgata
     3601 cacactcaaa atcttttaat attattggtt tatcacatct acaagaagca attgcaagtg
     3661 tcatagcaat tctatggtct ttgtgactcc acacctctac tccaccattt aacttgctta
     3721 ctccttcgat tatcaaacta tcctctttct cctctatatt tgctccaagt ttacttaact
     3781 caacatttat tgcatgaagt ctatcacatt ctttaattct aagcctacca gcattaatta
     3841 ttgtagtcct ccctatactt aaggatgcta ctacagataa tacaggtatt atatctgggc
     3901 attgagatgc atctatcaaa gtactattta aaccatttgt tattcctttt attttgttgc
     3961 cttctcttaa tatttccatt cccatcctag ataaaatttc aactacttca ctatcccctt
     4021 gaagagaatc ttctttaagg tctaatatgc taatatcttc tcctatagca tctgctgata
     4081 aataaaatgc tccttgagaa taatctcctt ctactttata atctctcgct ttatacgttt
     4141 gatttccttt tataataaat tctttatagt tattgtttat aatttctacc ccaaaatctt
     4201 ttatcgttga aagagttaaa tctaaatatc ctttagattc tagctcagta gtaattatta
     4261 tctttgaatc actctctagt gtaggcaata taaacaatag acctgtgata aactgagaac
     4321 ttatatttcc ttttactcta aattcatctg gtttaagctt acctgatata atcaaatcta
     4381 actgattttc tttgtaacta tattttatat tctgtctgtc aaatatctca taataagtat
     4441 caagtggtct ttttccaaga ttacctctac ctataaacct ttttacacca tcaaatgcta
     4501 aagatatagg tactaaaaat cttaatgtag agcctgattc attacaatct attgtcaatt
     4561 taggttgatt caactgattc tctctattta aaataccttc tttagaaaaa attcccgaaa
     4621 tttctaaaat atcttctttc ttttctataa tagctcctaa tgatgtcatt gctcttatag
     4681 tagctattat atcatcagaa aaatctatat tagaaattct actttttcca ttgcttagag
     4741 atgaacaaat aactgcccta tgtgccatac tttttgatgg tggtattttt acatctccag
     4801 ataatttact tggatatatt tttagacttc ccattatctt acctcctgaa aaaatttaga
     4861 agtagtatta tatataatac tctctccaat atcttttaaa agtattattt ttaatgtatc
     4921 acctagattt tttttatcaa gagatatagc atctactata gatttattat ctcttaattc
     4981 aatctcataa ggcaaattat aattcattaa aattgatttt ataagttctg atgtaccttt
     5041 ttttgtaatt cctatctttt cactttctaa agttatctta tacattccta tggcaacagc
     5101 ttctccatgg gtataacccg taaaattgta gtatttctca atagcatgac ctaatgtatg
     5161 gccaaagttt agaagcattc tatctccaaa gtctttttca tcagcttcaa ctacagtttt
     5221 tttaataaag caacatttat gtataatgta ttctatatgt tgcattactt cttctctatt
     5281 ttttaaactt aaaagtttat tgaaaaaagt tttgtctttt atacatccat attttataac
     5341 ttctgccatt ccatctctat agaatttttc tggaagtgat aataatacat ttgggtcgat
     5401 aaacacagct tttggttggt aaaagcttcc cactaaattt ttgcctctct caaggtcaac
     5461 tccaactttt ccacctacag aactatcaac ttgtgctaat aaagatgttg gaatttgaat
     5521 aaaacttata cctcttaaaa aagttgctgc tgcaaatcca cctaaatcac ctattacccc
     5581 tcctcctaaa gtaataatca aatcttttct agttaactta aagtcaagta aactatcgta
     5641 tattttagga agagtattaa atgattttgt ttcttcacca gattttaata ttaaagattt
     5701 aatttcaaat ccagattcac ttaaattatt ttttacttta tccaagtata attttgctat
     5761 attttcatca gttacaataa aaatcttatt tccaaaatat atttttttta tttcttctcc
     5821 tatattcttt agtatatttt ttttaataaa tatattgtag ctattttctt ttaaatttac
     5881 taatagtttt tcttccacgt ttaaccatcc ttttatagat tatatttctc taccaactgc
     5941 tgatgcgata gtctttaatt cacttatcaa ttcatcatat tcatttggct taatcgattg
     6001 ttttccatca caaagtgcat gtgctgggtc attatgaact tctattatta gaccatctgc
     6061 tccaactgca attgctgcct tagataaaga gtcaaccatc catgatttac ctgctgcatg
     6121 acttgggtca actataactg gtaaatgact aagtttcttc acagctaaaa ttgcacttaa
     6181 atccaaagta tttctagtat atgtttcaaa agttcttata cctctttcac aaagaactac
     6241 attttcattt cctccagcca ttatatactc tgctgacatt agccactctt caattgtagc
     6301 agataatcct ctttttaaaa gtattgtttt atttgtttta cctaattctt ttaataaatc
     6361 aaagttctgc atgtttctag ctccaacttg aattacatct acattttctt caaatatatc
     6421 tatatcttgt gttgacatta tttctgtaac tataggaagc cctgtttttt cttttgcttt
     6481 ttttaataaa tccaatccat catatttcaa tccttggaaa gcgtatggtg atgttcttgg
     6541 tttgaatgct ccacctctca aaaatcctgc acctgatttt ttcacatctt tagctataga
     6601 tactatttgg tcttctgttt caacagaaca aggcccagct atcatagcaa ttttctttga
     6661 accaatttcc attccattaa catttatgat tgaaggttct ggatgaaatt ttctattggc
     6721 ctttttaaaa ggctctacta catgcataac tttttcaact atcttatttg cttctattct
     6781 tttggcatct attttactag tatctccaac taaacctaaa acagttaatt ctgttccatc
     6841 tatgggatta acttctactc cttctctccc aatagcctca attaattttt taacttcatt
     6901 tttatctgct cccattttta atactactat catttttact acctcccaat attataattt
     6961 tatatttaaa ataaaaaaga gaactcacta atgagttctc taaaataatc tatcaagcac
     7021 taaatcttat ataaatatat aaaataatca tttttataaa atttacttat gctcaaaatc
     7081 actaaaataa ggcctcacca gaaaggaagt acatatattt ttgtaaaaga aatagccagc
     7141 gctaaaataa aagccccaga acataaagta aaaaaagcta taaagttttg ctgttagttc
     7201 ttttaatatt cctttcatag tattatcctc catttttata atttattatt cttcttgttt
     7261 aataattata acaaagaaaa tataattgtc aatattttct ataaatattt tcaatttaaa
     7321 atattttttt atttacttaa atcagcttct caattgatgt atttttataa aatcaattaa
     7381 acatcatctg taatttacaa taattttttt attttaaata ttataattcc aataaaaatt
     7441 atacatagca taaaaaataa agttgctgga tatccataaa tccagtttaa ttctggcata
     7501 tatctaaagt tcattccata tataccagtt ataattgtaa gtggtgctga tatggctgtt
     7561 aacaaggtta attttgtaat taagttattt gttttctcac ctactctgga agaataaata
     7621 tctaataatt tatctgctaa ctctcgtgtt gacaatgcga aattgtataa tttgtcaatt
     7681 ccaaaatcta taccttgaaa attttctaaa ttatattttt ttacatttga atattttaaa
     7741 tatcttatat tttccttaac tattctatcc ccaatataca aaagaggtct agtattctta
     7801 actataattc ttgttaatcc tcttacatca cttattctag atacatgatt cttattaatg
     7861 ttgtccatca tttcatcttc tatttgaaga attaactctt caactttttc caaagactca
     7921 aaaccatttt ttataacttc tctaagaatc aagtagttta tcttaaatag attaataact
     7981 ggattttttt caagttgaga gttcttcaaa ataatatttt ttacaaattc aaataaaaag
     8041 tgttcttcat tcactacaac caatataaaa ttatctgata aatacatatt tacttcttct
     8101 ataacagctt ctccatctct tagttcaaat gtatttaggc ttaaaaaatc gtaattatca
     8161 aataaatcaa gtttggttat ttcatcaaat ttaagacaat cattaaaagt tatttcatct
     8221 atatccaaag tttccttaag caattttaaa ttttttggag cacttaatat aagataagaa
     8281 ttttctgcta cataaaattt atctctaaaa tcttcaatag tctctcttgt atttaaattc
     8341 agtatataca tacatctact cctaaatttc aatttacatt ctaaaaattt tttataatcc
     8401 ttactgtaat tattttacac tattttaaca attattattt aatatttaca ttaaatttat
     8461 tataaggtaa gtgttaaata catttattat ttaaccaaag tttttattac agattacact
     8521 atatacttat tttaaaacat atttcatatt attgatacta aaaagctaaa aaataaaatg
     8581 catgtgctaa actgacaaca agcacataca cttcattctt attttaacta aactatataa
     8641 ttttaatata tattaataag cacataatct aataattaat atattacaac tattcatcat
     8701 ttaatctgta tcctacacca acttctgtaa ttatatatct tgggtttgca ggttgttttt
     8761 ctatttttcg tcttaatgtt gccataaaga cccttaaaga cttagtctca ttccctataa
     8821 cactacccca tatttctttt attataaaat tatgagtaag cacttttcca tgatgctttg
     8881 caagtaatga aagaatatta tattcaatag gtgtcaaatg gacttcaaca tcatcaacaa
     8941 taacttttct tttatcaaaa tcaatcaata atttatctac ttcaaacgta gatttaacat
     9001 catctttttg ttgagcttct acttgtgaat gtctaaatgc gactcttact cttgctaata
     9061 actctactat ggagaatggt tttgttaagt aatcatctgc ccctgcatca aatacttcta
     9121 ccttatctct atcttgctct ctagctgaaa ctacaattat tggtatattt gaaaattgtc
     9181 tgatagcttt tattacctct attccatcca tatcttctaa gcctaaatct aaaagaacaa
     9241 catctggaga atatgagaca gcaatagata taccttcttt tccagttata gcctccttga
     9301 catcataatc ctgtgttaaa agtgcagtag atataaattt tcttattgtc ttatcatcct
     9361 ctactaacaa tataagtttt ttactcattt cttcctccaa aactaatttt aaaatttaaa
     9421 actattcatt ttccttaggc aaactaaact taaaagttga acccttatct ttattgtttt
     9481 ctaccataat ttcaccttta tgagcttgaa ttatagattt acaaattgat aatccaagtc
     9541 caacaccctt tctggaatct ttagctcctt cttctccagt aaaaaatctg tcaaagatat
     9601 gctttttaag ttcctttgat ataccaggac cattatcaga tacttcaaac caaacataat
     9661 catctttctc gtatacattt atctctatct tacaatcttc ctttgaatat ttcaaagaat
     9721 tatctattaa attaattaaa acttgttcta ttaattttgc atccatagga acaaataata
     9781 tctgttctgg tatattaata tctataagtg cattttcaat tctttttttt attttttgta
     9841 acgcttctga tataatttcc tcaaccagtt cagaatgttt tttaacttcc aatttccctt
     9901 catctatctt ggtcatacta agtaaatttt caacaagtct tattaaccac tgagtatctt
     9961 catatacacc actaagtaat tcatctatta tatcttgacc tatagtaccc ttgtttttta
    10021 taattgtact tactgcacca gatattcctg ctagtggagt tctaaggtca tgcgaaactg
    10081 ctcttaaaag attacttctt aatctttccc tttcaatctc aagattggta ttttctttag
    10141 cttctgacaa gatttctcta tcaagcgcta ttgccatttg agcaattatt gtttcaatta
    10201 agcttatatc ttctgtatcc aattttgaat caatacatga tattcctact actcctaaag
    10261 ttaaattcat tcctacaatt ggtatatagt atccctttga accaggaagt gtatccgtgt
    10321 catttcctgc cactgttgaa ttggataaaa cccaacttgc aacagctttt tcatcttcac
    10381 tatccaaatc atttttatcc ttattattag tatttttttt gtaaagtttt gatttattat
    10441 gcttatctac atttaaataa caaattacat ctctacttaa gctaagagac aaaagttcta
    10501 tacctttgtt tactatatca tctttacttg ataatcttaa aaatccctta attatttgat
    10561 atatcatttt tgtattttct tctcttttag cagcagtttc tgcttcatgc tgtatcttag
    10621 acgtaagagt gctagttatt atacctacta ttgagaatac aaccagtgtt actatatagt
    10681 ttgaatctgc tatagataat gtatataatg gtgctgtaaa aaaataattt aaaagaacta
    10741 tattaaatac agaacttatt atacctgtac tatacccttt agtccacata gaaactaaaa
    10801 ctacacccaa catatatatt aacaatatat tttctcttat aaatcccatg ctttgtactg
    10861 catatgctaa aatacttatc atcaaagtaa taaatataag ttttataaaa tcaactttgc
    10921 taattttaaa tttagatttt attgatgatt tatccttttt aggtttgtat ttaacatctg
    10981 aggcaaattt atatggaatt atatgtatat ctatataatc aacttcatca attaatttgt
    11041 caactatatc ttttttaaat cttttactaa atcttttatt acttgaatga tttctaccta
    11101 ttacaatttt tgttacattt ctcagttttg caattctaag tatttgctct attatatttt
    11161 caccatgtaa aacaattatt tctccaccta atttcttagc taattttagg ttattttgta
    11221 gttgtttttg ttcatcaaat ggaagtcttg ctgtgtttgg agtttcaaca tataaagcta
    11281 tccattttgc aaatgtactg tctgatattc tggatgcagt tctaataact tttgctgaag
    11341 atggagatgg acttatacat gcaagaagtg tatctgatgt aggtattaca gttacatcac
    11401 cctttgatag tctggtcatt tgaacttctt tattaactct ctctgctgtt tttctaagtg
    11461 cgatttctct aagtgcaacc aggttatctt ttataaaaaa attatttttt gctctagtag
    11521 cttgttcttt cttatatatc ttaccttcat tgaacctttc aagaagtaca tcaggttcaa
    11581 catctataag ttctaactgt gtatttgtat ccaaaaactt atctggtata gtctctttaa
    11641 ctgatacgtt tgtaattacc tcaacaatgt catttagact ttctaaatgt tgtacattta
    11701 cagtagtgta gacatttatg cctgctaaca gcaattcttc tatatcttgc catctttttg
    11761 tatgccttaa cccagcaaca ttagaatggg caaattcatc tactaatata acttctggtt
    11821 ttcttgctaa agcagcatct aaatcaaatt cttttagttt tatagattta tattctatct
    11881 cctttacagg aagaatttcc aagccatcaa gtagagccaa tgtttctggt ctagtgtgtg
    11941 gttcaatata tccaactact acatctacac ccactttttt taaattttgt gcagattcca
    12001 acatagagta ggtttttcct actccagctg cataaccaaa gaatattttt aattgacctc
    12061 tacttagttt ttcttcttca tttttaattt tttctaatag aatatcagga ttaggtctat
    12121 ccattaaaaa acctccttac accattcatt actattataa tatcaattcc gatataaaat
    12181 tgtagttaag atgtattttt aatgattaag attatgtaaa gatttaaata tgaatttaaa
    12241 aaatttagtt gtaaaataaa aatgcctaac aataggcatt tttatcttgt gtataatcat
    12301 tttagtttaa gttaagtatt tccataaact catcacctgt aatagtttct ttttctaata
    12361 aataattagc aagttcatgc aatttatcaa tattattttc taagatttca attgcatttt
    12421 tgtgacaagc ctttactatt ttaaatacct cttcatctat tttagcagca gtgtcggaag
    12481 aacaagcaag agaagtatct cctcctaaat attgattgct tacagtttca agagcgacca
    12541 tatcaaactc ttcactcata ccaaggcgag taaccatagc tcttgcaatt ttagtagctt
    12601 gttctatatc atttgatgct cctgatgtac aagtgttaaa gataacctct tcagcagcac
    12661 gaccacctgt aaaagtagtt attttatcaa ttgcttcttc tttagacata agtacactct
    12721 catcttcagc aacttgcata gtatatccca atgctcctga agttctaggt attatagtaa
    12781 tcttatggac aggtgcagaa tgtttttgca tagcagcaac aagtgcatga cctatttcat
    12841 gatatgctat aattttcttt tccttatctg aaattacagc tcctttacgt tgatacccag
    12901 caattacagt ttcaacagat tcatcaaaat cctgttgttc tactatatct ctgccagact
    12961 taactgctaa aagtgctgct tcgtttacaa tatttgcaag ttctgcacct gatgctcctg
    13021 atgtagacct tgcaattgca ttataatcta tatctgagct tacatttaca ttttgagcat
    13081 gtacctttaa tatagcttca cgtcctccaa ggtctggaag ttcaactggt atacgtctat
    13141 caaatcttcc tggacgaagt aatgctttgt caagactgtc aggtctattt gtagcagcaa
    13201 gtattactac tccaattcca ccatcaaaac catccatctc acttaacaac tgatttaaag
    13261 tttgttcacg ttcatcattt ccacctaatc cacttccatt atcacgcttt ttaccaatag
    13321 tatcaatctc atctataaac actatacatg gagctttttc ttttgcttgt ttaaataaat
    13381 cacgaatcct agaagctccc attcctacaa acatctcaac aaactcagaa ccagatattg
    13441 agaaaaatgg tacatttgct tcacctgcta cagcttttgc aaggagtgtt tttcctgtac
    13501 ctggaggtcc tactaaaagt gctcctttag gcatttgtgc tcctatcttt ttatactttt
    13561 ctggattgtg taagaaatct actatttcag ttaaagcttc ttttgcctca tcttgaccag
    13621 ctacatcatt aaatcttttt ccactttgag cagatacata cattttggcg ttgctttttc
    13681 caaattgcat agcattacca cccattcgct tttgcataga cttcatcatt aagttaccaa
    13741 atagcataag aatacctata ggtattatcc atgtaagtaa aatacttaaa agaggtgaat
    13801 tttccttagg aataacctta gtaaattcta cttttgcatt ttttagtcta tctaccaatt
    13861 ctgggtcatc cattctacca gttatgtata tattcttatc cttgctacta gatggattta
    13921 ttgctattct attattttct atctcaacag atttaacctt tcctgtatcc aacattgtga
    13981 gaaactttcc atagtcaatc tcaaccattt ttttatccat taaacttgga aatattaaag
    14041 catttaaaaa cattactact attattataa ctgtataata atagagtgct gacctttttt
    14101 ggggtttatg ttcatccata attttattct cctttaaatt atttttatgt tatttgcaat
    14161 caatgtaagt taaaagttta tttaatttct ctaaaccttc tattatatct tccatatctt
    14221 gcaatttttc actggtcata ttttcaaaat aactatcaaa aatactactt gaaatatgtt
    14281 cttgtatatc caatgattta tttgtaactt ttacttttac aattcgttgg tcttctatat
    14341 ctcttatgcg atttaaaaag ccattttttt caagcctttg acagattact gatacattac
    14401 tatttgtcat gtttaagttc ttccctaaat cgcttatttt tttctctcca tgttcacata
    14461 actcagataa aatcaaagct tgcactgctg taataccaag tggctgataa aaatcactta
    14521 ataaagttgt aaatttcata tttatatctc tagtcaactc tagtagctct ttcttaaaac
    14581 tcatctcatc catacaaacg ctccctacga aaatacttta tttcaaattt aggctgaagt
    14641 gcatactgta aataaatact taatactata atgtttaatg tattaaatat tatagtatta
    14701 agtatttatt gtcaattaca atattgtaac ttcaattgga attttgttat cttattgata
    14761 tttaatttta tacaggatat ttttaataaa atttaataaa aaatcgatat atatatttta
    14821 cactgatttc tcccttttta atctctttta aaagttaaaa tacaaattta ttgatactat
    14881 aaaggtttag tctcccatta tcgcaccata tataaaatta aaaccacctc tatctacata
    14941 cacctaaagt gattttctta aaatataaaa ctactattat aaggttaaat ttaaaaaaaa
    15001 taaggtataa agtatcatta agattaactt tatacctata aatttctata tttttttagt
    15061 taacatttat atactcttaa aatttattgt atcaaatagt tttttatcca atttctaaac
    15121 aacaattctg aatgtttttt ccattttaca gttggctctt ctgaagggtc atcattaata
    15181 tagtaatttt gaggtataga tattttatct cctttattta tatccctctt atattcttta
    15241 tcaagagtgt atttgtcata ctcgctatga cccattacga acacatctct tgaatttgta
    15301 attatatagg gaccagcttc tttagaatga ctcacaatgc tcaactcttt attctctttc
    15361 aaagcttcaa tatttacagt agtatgtctt gaatgtggag caaaaaattc attttcaaat
    15421 ccatctacta ttttactatc tttatctacc ttatgcttaa atactccaaa acatttttga
    15481 cttagtggaa gtttttttac attgtagtat ttgtaaagac ttgcttgagc tgcccaacat
    15541 atatataatg tagatttagt ttttctctta gaataatcca taatttcttc tagttcattc
    15601 caataatcaa cttcttcaaa ctccatttgt tcaactggag ctcctgttat tataaagcca
    15661 tcaaaatact ctcctttggc ttcatcaaat gttttataaa attctttaac atattcatta
    15721 caacttcttt tactttctct tgttgaaatt ttaataaatt caacagacac gttgaatcct
    15781 gttttatcta tccttcttag taaatctaat tctgtatcta tctttatagg cattagattt
    15841 agtatagcta ttttagtgtc tatgttttct tcatacttta attccttttt aaaatcttct
    15901 ttataaatta catctattcc ttcatctaat aatttattaa taacaggtaa tccctttggt
    15961 agtattaaag ccatatcttt tcacttctct ttctcttaga atattagaaa tttaaagctt
    16021 gatttaaatc agctattaaa tcctctacat tttctattcc aacagataat cttattagtg
    16081 aaggcaatac tccagatgca attaattgtt cttctgttaa ttgtctatga gtagttgaag
    16141 ctggatgtat aacacaagtt cttacatctg aaacatgtgt aaccaaagat gctatctgta
    16201 atttttccac aaattttttg gcattttcta acccacctct tactccaaat gataaaacac
    16261 cactacatcc tcttgataaa tactttttgg cattctcata atacttgtta tcttcaagtc
    16321 ctgggtaatt aatccaatct acattttcat gtttttctaa aaatctagct attttaagtg
    16381 cattttcact atgtctctcc attctaagat gtagtgtttc aacatttaaa ttagtaagat
    16441 acgcattgaa tgggcttaaa caatttccat agtctctaag caactgaact cttgctttag
    16501 ttgcatatgc ggcatttcca aatttttgag tatagcttat tccatgatat gttgggtctg
    16561 gctcaactaa ttctggaaat tttccattat cccagttaaa gtttccacca tccactataa
    16621 ttcctccaac acttgaagca tgcccatcta aatattttgt ggtagaatga gtaactatat
    16681 ttgctccata ctttaaaggg ttacaaagca ctggagatgc taatgaatta tcaacaataa
    16741 atggaacatt aattctctta gctacatttg ctattttttc aaaatctatg acttcaagag
    16801 tcggatttgc aagtgtttct gcaaatacaa ccttagtatt ttcctttgca agttctacta
    16861 tctcatcttc acttgaatct aagtcaaacg atattaaatc tataccaaat tttttaagac
    16921 taggtcctag taaattgaat gttcctccat atacttttga agaacaaagt atactatctc
    16981 ctgatttaca tatatttaaa actgccaaca tatttgcaga ttgccctgat gatacagcta
    17041 cagaagatac tccaccctct agtaaactta ttttttcttc aaaagcttga atagtaggat
    17101 tgcttattct tgaatatata tgtccatcaa cttttaaatc aaataattca gcaagttggt
    17161 ctatactgct atatttaaaa gttgtacttt gatataaagg taatactctt ggttctccat
    17221 tacctggttt ataatttcct tgcacacata ttgtttcttt attatacatt cttgtatccc
    17281 ccttagattt tagtaaaaaa aattaaacta tttatcagat gagagttaaa ataaataaag
    17341 cctgagtata taactcaggc tctgtataag cttaattata ctcatctctc agctttcgcc
    17401 gcaggattta gcaccaatgt atataaatat actggttgcc gggtttcata gggccagtcc
    17461 ctccacctct ctggataaga tttatttaat ttttctatgt tttatatctt atatattttt
    17521 cctaattttg tcaactattt ttttcttttt tttcttttaa taattttccc aacctctact
    17581 tgaattacag acattatggc taaaacaatc gttataagcc actctaagtt atttaaaact
    17641 gttaagttaa aagcatttct aaaaacaggt acaaacagta ttactgctat taaaaatgca
    17701 gaagttaaaa atgagattaa aagaaatgga tttttaccat tatttctatt aaacattgaa
    17761 tctgtatttg aacgctgact aaatgctcgt aacatctgtg aaaatgctaa tacacaaaat
    17821 gccattgttt gacctacaga atgattttct atattaagac caattttata tgctaaaata
    17881 gtcattattg ccacaaatat accttgggta acaactcttt gtataagatt tttttcaaac
    17941 aaagtggttg atttcactgg ctggtgcttc attatatttc tgcttggtgg gtcaactcca
    18001 agagcaaggg ctggaagcgt agcagttgca aggtttaccc atagaatatg aacagcaaga
    18061 agcggagtat cccaatttaa taatattgct acaaacaacg taagtatctc tgctatatta
    18121 ccaacaagca aaaattgtat aactttttgt atgtttctat atacacgtct accttctttt
    18181 attgcataag caattgttgt aaaactatcg tccattaaaa tcatgtcaga agaatctttt
    18241 gcaacatctg taccacttat acccatggct ataccaatat cagcagactt taaggcagga
    18301 gagtcattta caccatcccc agtcattgct ggtacttctc caattctttt tagagattgt
    18361 ataattctaa gtttatcaaa cggggacaca cgagcaaata ctgtagtatt tttaacagct
    18421 tcatctagtt catcatcact aaaattatca agttcatcac cagatatcac agtattttct
    18481 tcactccata ttcctaactc atgagcgatt gttgtggcag taactttatg gtcacctgtt
    18541 atcattatgg ttcttatacc tgcttcttta caagtactta ctgcatcaat tacctcagtt
    18601 cttggtgggt caatcatacc aaccatacct ataaatgtca aatcatattc tatattttca
    18661 ctatcttctt ttggtataga tgaaatctca cgcttagcaa aaccaagtac acgcaatgca
    18721 tctttagaca tgttaagaca taattcgtgt atattctttt tatccacttc ggtaatgttt
    18781 ctttcacctt ttgaagttaa tattttagta cataggtcga gaatttcgtc tactgctcct
    18841 ttagtataag caataatctt ttcatctata ttatgaacag ttgtcataca ttttctatct
    18901 gaatcaaaag gctgttcaaa tacacgtgga tattcttctt caagagcttc ttgatctatt
    18961 ccaaattttt tggccataaa aattaatgcg ccctctgttg ggtcacctaa tatttcatct
    19021 gttttatctg gattaataca tgcatcatta caaagtgcac cagcataaac aatctcttta
    19081 tacactaatg gatgtttttc agatgcagta tctattgatg tcacttttcc agattcaaaa
    19141 tctccattga cagcaatatg tgtaacagtc attttattta aggttaatgt accagtttta
    19201 tcacaacata ttactgtagc tcctcctaat gtttcaacag caggtaattt tctaattaaa
    19261 gcatttcttt tagccatacg ctgtacgcct aaagccatta caatagtagc tgttgcaggt
    19321 aatccctctg gaataattga tatggcaagg gaaattgcaa tcataaacat tggcaagaga
    19381 ggtctctgat aaaatgcacc tattgcaaat ataacaacgc atacaataat acctatgaca
    19441 cttaatgtct ttccaacagt attaagttta cgttttatag gagtatcaaa atcatcttgt
    19501 gaatctaaaa gatgtgctat gttacccact tctgtatcca taccagttgc tactacaaca
    19561 cctacacctc taccatatgt gactattgaa gatgtatacg ccatattgct acggtctcct
    19621 aatacacatt ctttaggtaa gattgtatct gcatcttttt ctgatggaac agattctcct
    19681 gttaaagaag cctcttgaat ttttaaactt gatgtttcta ttaaacgaag gtctgctgga
    19741 accatagctc catcttctaa aaaaacaata tctcctacta caagattact tgctggaata
    19801 acattttctt caccatcacg cataacacga gctgtaggtg agttcatatt ttttaatgct
    19861 tctaatgatg attctgcttt tttctcttga acaattccaa taactgcatt tactgttaca
    19921 attattagta ttacaaaagc ttctgtaaat tcgccaaata tcaaagataa tattgctgct
    19981 cctattagta tcataaccat agcatctgta atttgactcc aaagcatttg taaagttgtt
    20041 ctaggtggtt tttttctaag ttcatttaag ccattctctt taagccgttt ttccgcctcg
    20101 gtatcggtga gcccctcctc tgatgaatct agactttcta aaatttcatg cgttgctctt
    20161 gaatgccaag gcatctgttt gtgattttcc attttggaag accactcctt tcttttttta
    20221 atttaagaat aattatattt taaaatcaag ttcaattcaa tacacactaa gtatatttta
    20281 cctaaattta acaatgccat ttaatattta acaaaacttc tactctttta caaatatgta
    20341 ttcactattg ttagataagt tttctttata cttataacct tctaaattaa actttttcag
    20401 ttcttcaata tctcttatcc tattttttac aatgtatttt gtcatcatac ctctagcttt
    20461 ctttgcttgt gtagattcca ctttcaatat gccattttta tttactttaa aagtacatgt
    20521 aatatatgta tcactatctt taataaattt ttctatggac ttgctgtatt catttgatga
    20581 aagattaatg atagctgttt tttctttgat tagttcttta tatatacgat ttccccaata
    20641 ttcgtataaa ttcttaaact cacctactcg taattttgtc tgcatttcta atctatattc
    20701 atatatacta tcatatggtt ttacaacacc atacaatcca cttatgattc gaatacagct
    20761 atttgcaaac tcttcatctt tccttgtaaa attttctgct tctatatttt tatattgtat
    20821 tccatcataa gttaagattg cagggtttcc taatctatca aatttaatgc tttcaaatct
    20881 atttttattt aatacagcta atttttcatt aattttcatt ttatttttaa aatcttctat
    20941 accaaatgtc tttatatttt ccactatttc ttttgtgtta tctataaaac aaggcaaact
    21001 caaatctcta tcaaatactt ctatattctt catattttta gctggtgata acattataat
    21061 cataaattct tattactcct gtttttccaa atattcaaga atctcttgtg catttgtgtc
    21121 tggttttgct ttttcaaaga ctttttcaat aattccattt tcatctataa tatatgtcgc
    21181 acgtacaact cccaatgcag tttttccata catttttttc tctttccata catcaaaagc
    21241 ttttatagca actaagtcag ggtcagaaag taaaataaaa ggaagttcat gcttttctgc
    21301 aaatttttga tgtgatttta tactatcttt acttatccca ataacttgta tatcttcctt
    21361 tttaaatcca tcatatgcat ttctaaaagc acaagcttga cgtgtacatc ctggtgtatt
    21421 atctttagga taaaaatata ctactacttt tttgccttta aaatctgaca tacttacctt
    21481 atttccatct ttatcttcta gtgtaaattc tggtgctttt gttcctatac tcaacataaa
    21541 tatctctcct tttgttttta ttaataattt tatactatgc ttatattatt accctataaa
    21601 tctatatata aataaaatat tttatttttt atgtataaag tttcataaat atataatttt
    21661 catgtattta tattctaaaa tagactgaaa
//
LOCUS       DACTGW010000049        20752 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.049, whole genome shotgun sequence.
ACCESSION   DACTGW010000049 DACTGW010000000
VERSION     DACTGW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.049"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            358..669
                     /gene="rpsJ"
                     /locus_tag="I9573_001420"
     CDS             358..669
                     /gene="rpsJ"
                     /locus_tag="I9573_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAT4796046.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            761..1390
                     /gene="rplC"
                     /locus_tag="I9573_001421"
     CDS             761..1390
                     /gene="rplC"
                     /locus_tag="I9573_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAT4796047.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1420..2043
                     /gene="rplD"
                     /locus_tag="I9573_001422"
     CDS             1420..2043
                     /gene="rplD"
                     /locus_tag="I9573_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAT4796048.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2043..2333
                     /gene="rplW"
                     /locus_tag="I9573_001423"
     CDS             2043..2333
                     /gene="rplW"
                     /locus_tag="I9573_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007791436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAT4796049.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            2363..3193
                     /gene="rplB"
                     /locus_tag="I9573_001424"
     CDS             2363..3193
                     /gene="rplB"
                     /locus_tag="I9573_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAT4796050.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3228..3509
                     /gene="rpsS"
                     /locus_tag="I9573_001425"
     CDS             3228..3509
                     /gene="rpsS"
                     /locus_tag="I9573_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAT4796051.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3541..3876
                     /gene="rplV"
                     /locus_tag="I9573_001426"
     CDS             3541..3876
                     /gene="rplV"
                     /locus_tag="I9573_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAT4796052.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3899..4714
                     /gene="rpsC"
                     /locus_tag="I9573_001427"
     CDS             3899..4714
                     /gene="rpsC"
                     /locus_tag="I9573_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAT4796053.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4750..5181
                     /gene="rplP"
                     /locus_tag="I9573_001428"
     CDS             4750..5181
                     /gene="rplP"
                     /locus_tag="I9573_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007791551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAT4796054.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5183..5386
                     /gene="rpmC"
                     /locus_tag="I9573_001429"
     CDS             5183..5386
                     /gene="rpmC"
                     /locus_tag="I9573_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAT4796055.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5410..5664
                     /gene="rpsQ"
                     /locus_tag="I9573_001430"
     CDS             5410..5664
                     /gene="rpsQ"
                     /locus_tag="I9573_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAT4796056.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5690..6058
                     /gene="rplN"
                     /locus_tag="I9573_001431"
     CDS             5690..6058
                     /gene="rplN"
                     /locus_tag="I9573_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAT4796057.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6082..6387
                     /gene="rplX"
                     /locus_tag="I9573_001432"
     CDS             6082..6387
                     /gene="rplX"
                     /locus_tag="I9573_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAT4796058.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6417..6959
                     /gene="rplE"
                     /locus_tag="I9573_001433"
     CDS             6417..6959
                     /gene="rplE"
                     /locus_tag="I9573_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAT4796059.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6977..7162
                     /locus_tag="I9573_001434"
     CDS             6977..7162
                     /locus_tag="I9573_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAT4796060.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7195..7593
                     /gene="rpsH"
                     /locus_tag="I9573_001435"
     CDS             7195..7593
                     /gene="rpsH"
                     /locus_tag="I9573_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAT4796061.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7624..8166
                     /gene="rplF"
                     /locus_tag="I9573_001436"
     CDS             7624..8166
                     /gene="rplF"
                     /locus_tag="I9573_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAT4796062.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8202..8570
                     /gene="rplR"
                     /locus_tag="I9573_001437"
     CDS             8202..8570
                     /gene="rplR"
                     /locus_tag="I9573_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAT4796063.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8591..9100
                     /gene="rpsE"
                     /locus_tag="I9573_001438"
     CDS             8591..9100
                     /gene="rpsE"
                     /locus_tag="I9573_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAT4796064.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9115..9300
                     /gene="rpmD"
                     /locus_tag="I9573_001439"
     CDS             9115..9300
                     /gene="rpmD"
                     /locus_tag="I9573_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAT4796065.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9333..9776
                     /gene="rplO"
                     /locus_tag="I9573_001440"
     CDS             9333..9776
                     /gene="rplO"
                     /locus_tag="I9573_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAT4796066.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9820..11088
                     /gene="secY"
                     /locus_tag="I9573_001441"
     CDS             9820..11088
                     /gene="secY"
                     /locus_tag="I9573_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAT4796067.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11105..11755
                     /locus_tag="I9573_001442"
     CDS             11105..11755
                     /locus_tag="I9573_001442"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAT4796068.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11756..12502
                     /gene="map"
                     /locus_tag="I9573_001443"
     CDS             11756..12502
                     /gene="map"
                     /locus_tag="I9573_001443"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005367749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAT4796069.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12524..12793
                     /locus_tag="I9573_001444"
     CDS             12524..12793
                     /locus_tag="I9573_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HAT4796070.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12831..13049
                     /gene="infA"
                     /locus_tag="I9573_001445"
     CDS             12831..13049
                     /gene="infA"
                     /locus_tag="I9573_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAT4796071.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13085..13198
                     /gene="rpmJ"
                     /locus_tag="I9573_001446"
     CDS             13085..13198
                     /gene="rpmJ"
                     /locus_tag="I9573_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAT4796072.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13351..13722
                     /gene="rpsM"
                     /locus_tag="I9573_001447"
     CDS             13351..13722
                     /gene="rpsM"
                     /locus_tag="I9573_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAT4796073.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13758..14156
                     /gene="rpsK"
                     /locus_tag="I9573_001448"
     CDS             13758..14156
                     /gene="rpsK"
                     /locus_tag="I9573_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007791581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAT4796074.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14188..14811
                     /gene="rpsD"
                     /locus_tag="I9573_001449"
     CDS             14188..14811
                     /gene="rpsD"
                     /locus_tag="I9573_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAT4796075.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14889..15836
                     /locus_tag="I9573_001450"
     CDS             14889..15836
                     /locus_tag="I9573_001450"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAT4796076.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15857..16198
                     /gene="rplQ"
                     /locus_tag="I9573_001451"
     CDS             15857..16198
                     /gene="rplQ"
                     /locus_tag="I9573_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007791394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAT4796077.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16318..17151
                     /locus_tag="I9573_001452"
     CDS             16318..17151
                     /locus_tag="I9573_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HAT4796078.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17139..18005
                     /locus_tag="I9573_001453"
     CDS             17139..18005
                     /locus_tag="I9573_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HAT4796079.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDISFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            17999..18802
                     /locus_tag="I9573_001454"
     CDS             17999..18802
                     /locus_tag="I9573_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAT4796080.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18834..19565
                     /gene="truA"
                     /locus_tag="I9573_001455"
     CDS             18834..19565
                     /gene="truA"
                     /locus_tag="I9573_001455"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAT4796081.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19685..20116
                     /gene="rplM"
                     /locus_tag="I9573_001456"
     CDS             19685..20116
                     /gene="rplM"
                     /locus_tag="I9573_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAT4796082.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20145..20537
                     /gene="rpsI"
                     /locus_tag="I9573_001457"
     CDS             20145..20537
                     /gene="rpsI"
                     /locus_tag="I9573_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAT4796083.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8287 a         2445 c         4325 g         5695 t
ORIGIN      
        1 atggatttaa tataaaaaaa agaggggggt aactcctctc ttttttgttt tttgttgaca
       61 agtagcgcta aagcatatat actaaactag tgccttgaga cagattaaca atcaagttta
      121 aggttatgat gttgtaatag aaaataaaat tgaaaaaaac agaaaaaaaa cttgaaaaaa
      181 tgtaacagat gatataaaat ataatagtgt gcttgagaga ttaagaaaat aataattaaa
      241 tcctagtgaa atacgaactg atatcgattt aaaagttaca acacacccgg agcagtgtat
      301 cggtattggt tgtacgcaac ttgaaaatta aacacgtgat aaaggaggga aaacaaaatg
      361 gctaagaatg aaaaaataag aataagatta aaatcatatg atcacaaatt attagatttt
      421 tcagcaggta aaatagttga aactgctaaa aaggctggat cacaagtttc aggacctgtg
      481 cctctaccaa cagaaaaaca agttgtaact atattaagag ctgtacataa gtacaaagac
      541 tctagagaac aatttgaaat aagaactcat aaaagactaa tcgacatagc taatccaaca
      601 cctaagacag ttgactcatt aatgagatta gacttaccag caggtgtaga tatagaaata
      661 aaattataag aacccctaag atattatcct atagatgatt atcttagaat gattactcta
      721 aaagatgtaa tccgctgtaa gaattatagg aggtgctaat atgaaaggaa tattaggcaa
      781 aaaagttggt atgactcaaa tattcactga taaaggtgta gttatacctg taacagctgt
      841 tgaagcaggg cctatggtcg ttactcaaat aaaaacagtt gataaagatg gatacaatgc
      901 tatacaaata ggttttgaag atgctaaaga aaaagcttta aacaaaccta aaaaaggaca
      961 tttagctgct gctaatgttt taaagaaaca tttaaaagaa tttagagtag attctgtaga
     1021 aggatacaca gtaggacaag aaataaaagc tgatgtattt gaagcaggtg caaaaataga
     1081 tgttactgga ataagtaaag gtaagggatt ccaaggtcca ataaaaagac atggacaaag
     1141 tagaggtcct gaaactcatg gttctagata ccacagaaga ccaggttcaa tgggagcttg
     1201 ttcttaccca ggtagagtat ttaaaaacaa aaaattagct ggacatatgg gaagcgtaaa
     1261 ggtaacagtt caaaacttag aggttgttaa agtagatgct gacaagaacc ttatattagt
     1321 taaaggagct ataccaggag ctaaaggttc agtagtaact ataaaagaag ctataaaggt
     1381 ttctaaataa tgactaacct agagaaagga ggaataacaa tgccaaaatt aaatgtacta
     1441 aatgttagtg gacaaaacgt tggagaaata gaattatcag attctatatt tggcgtagaa
     1501 gttaatggac atgttttata cgaagttgta aaaaatcaat tagcaaataa gagacaagga
     1561 actcaatctg ctaaaactag agcagaagtt agaggtggcg gaagaaaacc ttggaaacaa
     1621 aaaggaacag gtagagcaag acaaggttct acaagatctg tacaatgggt aggtggagga
     1681 gttgcttttg cacctaaacc aagaagctac aaatatacat tacctaagaa ggtaagaaga
     1741 ttagctatga agagtgcttt atcttcaaaa gttcaaaata gtgaagttat agtattagat
     1801 gcattaaaca tggatgctcc taagactaaa gaatttgctc aaatattaaa caatataaat
     1861 gctgctaaaa aagctttagt agttatagct gacaaaaacg ataacgtaat aaaatcagct
     1921 agaaatatag aaggtgttca aactgcatta gttaatacta tgaatgtata tgatatatta
     1981 aaatacgatt catttataat aactacagat gctgttaaaa aagtggagga ggtgtacgca
     2041 taatgactaa tccacatgat gtaataataa gaccagttgt aactgagcac agtatggctg
     2101 aaatgggtga aaaaaaatac acttttgttg tggctaaaga tgcgaacaaa actgaaataa
     2161 aaaaagcagt agaaaaagta ttcggagtta gtgttgataa agtaaatact ttaaactacg
     2221 atggaaaagt taaaagaatg ggtagaactc aaggaagaac agcaagcttt aagaaagcag
     2281 tagttaaatt aactgctgat agtaaagaaa tagaattctt ccaaggaatg tagtattata
     2341 acaaacgaag gagggaaaac agatggctat aaaaaagttt agaccaactt ctcctgcctt
     2401 aagacaaatg acagttttag tttctgatga aataacatgt aatcaaccag aaaaatctct
     2461 tttagttaac ttaaagaaaa atgctggtag aaacgtacat ggtagaataa ctgttcgtca
     2521 tagaggtgga ggacaaaaaa gaaaatatag aataatagat tttaaaagag ataaagatgg
     2581 tatacctgct aaggttgcta ctatagaata tgatccaaac agaactgcaa atatcgcatt
     2641 attaaactat gctgatggag aaaaaagata tatattagct ccagttggaa ttaacgttgg
     2701 agatactata ttatcaggat taggtgctga tataaaacca ggaaactgtt tagcattaaa
     2761 agatatgcca gttggtacta tcattcataa tatagaatta aagccaggaa aaggagctca
     2821 attagttaga tctgctggag tttctgcaca attaatggct aaagaaggaa aaaatgcttt
     2881 attaagatta ccatcaggtg agatgagatt agtaagcata aactgtaaag ctactatagg
     2941 tcaagttgga aatatagagc atggtaacgt agttatcggt aaagcaggta gaaaaagaca
     3001 tatgggaata agacctactg taagaggttc tgtaatgaac cctaatgacc atccacacgg
     3061 tggaggggaa ggtagatctc caataggtag accatctcct gttactccat ggggtaaacc
     3121 agctcttgga tacaaaacta gaaagaaaaa taaagcttca aataaactaa tagtatcaag
     3181 aagaactaag tagtaaattt atttttgctc ggaaaggagg aatataaatg tcaagatcaa
     3241 ctaaaaaagg gccttttgtc catgcaagac ttttaaagaa gatagaagct atgaatgcta
     3301 gtggaaataa agaagttata aaaacttggt caagatcttc aactgtattt ccacaaatgg
     3361 tagaaaacac tatagctgtt catgatggaa gaaaacatgt tccagtatat ataacagaag
     3421 atatggttgg acataaatta ggtgagttcg ttcctactag aactttcaaa ggacataagg
     3481 atgacgaaaa atctaataag agaaaataat taaattatga ctaagaaagg aggaataaca
     3541 atggaagcaa aagctactgc taaatacgta cgtgtatcac caagaaaagc aggccaaata
     3601 tgtgaccttg ttagaggaaa gaatgttgat gaagcattag caatattaaa gtttactcca
     3661 agaggagcag cttcaataat agctaaggtt gtaaaatcag ctaaagctaa cgcagaaaac
     3721 aatcacgaaa tggatactga aaaattatat atagcatcaa tagttgctaa tcaaggacca
     3781 acaatgaaga gattcatgcc tagagctatg ggtcgtgcaa ctacaataag aaaaagaact
     3841 tctcatatag aggttgttgt taaagaaaaa aaataattaa tagataggag ggaaagtaat
     3901 gggtcaaaag gttaatccac acggcttaag ggtcggtgtt ataaaagatt gggactcaag
     3961 atggttcgct actgataaaa aagagttcgg aaacttatta ttagaagacc ataatatacg
     4021 taaattctta aagaaaagat tatactcagc tggagttgct aagatagaaa tagaaagatc
     4081 agctaacaaa ataaaaatgg acttacacgt tgctaagcct ggtgtagtta taggaagagc
     4141 tggtgcagga atcgaagcat taaaagctga attagaaaaa atgacaaaga aaactataat
     4201 agttaatata gtagaagtaa gaagtacaga taaaaatgct caattagtag ctgaaaatat
     4261 agctttagct atagagagaa gggttgcttt cagaagagca atgaaacaag ctatacaaag
     4321 agcaatgaaa tctggtgcta aagggataaa agtttctgct tcaggaagat taggtggagc
     4381 tgaaatggct agaactgaag gttatagtga aggaaatgtg ccactacaaa ctttaagagc
     4441 agatatagat tatggtttcg ctgaagctga tacaacttat ggaaaaatcg gtataaaagt
     4501 ttggatatgt aatggtgaag ttttaccaac tagagacggt gtaaatccaa gagaagaaag
     4561 cagaaagagc gatagaagag acaacaagag agataataga agaaacgata gaagaggaaa
     4621 tgacagaaga ggaaatgata acagaggaaa ctacagagga caaagacctc aaggtggatc
     4681 aagacctcaa agaactgaaa acaaaggaaa ttaataatag ttaggttcaa ggaaggagga
     4741 aaaatactca tgttaatgcc aaaaagagta aagcgtcgta gagttcatag aggaagcatg
     4801 gctgggcaag ctcataaggg taataaagtt acttatggtg agtttggatt agtagcatta
     4861 gaagcttctt ggataacttc taatcaaata gaagctgcca gaatcgcgat gactagatat
     4921 ataaaaagag ggggaaaggt ttggataaaa atattccctc ataaaccagt aacaagaaaa
     4981 ccagcagaga ctcgtatggg tgcagggaaa ggttctccag aatattgggt agctgtagtt
     5041 aagccaggaa gagttatgtt tgaattagca ggtgtttctg aagataaagc aagagaagct
     5101 atgagacttg ctgcacataa attaccaatc aaatgtaaat ttgttaaaaa agaagattta
     5161 gaagtaaagg gtggtgaata ggatgaaagc taaagaatta agagatttaa caagcgaaga
     5221 gctaatgaac aaattaaacg acttcaaaag tgaattattt agcttaagat tccaattagc
     5281 tactggtcaa ttagaaaata cagctagaat aaagtttgtt aagaaggata tagcaaaagt
     5341 taaaactgtt cttgctgaaa gaaagttata cgaaactaga gcttaatttg gaaaggaggc
     5401 tttttaaaca tggaaagagg aagaagaaaa gttagaatag gccgtgttgt tagtgataag
     5461 atggataaaa caatagtagt agctgttgaa gaattcgtac gtcatccact atataataaa
     5521 cgtgttaaga gaactaagaa attcaaggct catgatgaaa agaatatatg taacatcgga
     5581 gatagagtta aaataatgga aactagacca ttatctaaag ataagagatt cagactagtt
     5641 gaagttgttg agaaagttaa gtagtttttg aagaaggagg gattacgata tgatacaaca
     5701 agaatcacgt ctaagagttg ctgataactc aggagctaaa gaacttttat gtatacgtgt
     5761 tctaggcgga agtaaaagaa gatatggtaa cataggcgac gttatagttg ctactgttaa
     5821 aagtgcaaca cctggtggag ttgtaaaaaa aggtaaggtt gttaaagctg ttatagtaag
     5881 aagcaaacaa ggcgtaagac gtaatgacgg aagctatata tcttttgatg aaaatgctgc
     5941 tgttataata aaggacgata aaactccagt aggaactcgt atattcgggc ctgttgctag
     6001 agagttaaga gacaatgaat tcatgaaaat agtttctctt gctccagaag tactataatt
     6061 aaggaggtgc aataggacat gatgcgtgtt aaaaaaggtg acactgttgt agttatagca
     6121 ggtaaagata aaggtaaaaa aggttcagtt ttaaaggtat atcctaaaac tagcaaagtg
     6181 ttagttgaag gtgtaaatgt aataactaaa catcaaaaac caagtgctat gaaccaacaa
     6241 ggtggaataa taaataaaga agccccaata cacatatcta atgtaatgcc atttgatcct
     6301 gaaacaggaa aaggcgttag agtaagatat gaagtaaaag atgggaacaa agtaagagta
     6361 tcagcaaaga gcggaaaaga attataataa atattgcttg aaaggaggga ccttaaatgg
     6421 cttctagatt acaagaaaaa tacatgaaag aagttgctcc tgctttaatg gagaaatttg
     6481 gatacaaaaa cgtaatggag atacctaagt taaataaaat agttattaac atgggtatag
     6541 gtgacgctag agaaaatcca aaaggattag aaaaaggcgt tgaagaatta gaaatgatat
     6601 caggacaaaa gcctgttata actaaagcta gaaaatctgt tgctaacttc aaattaagag
     6661 aaggaatgcc aataggaaca aaagttacat taagagctga caaaatgttc tactttatgg
     6721 acaaattagt ttcagtttct ttaccaagag ttagagattt tagaggagtt aaccctaatg
     6781 cttttgatgg tagaggaaat tatgctttag gagttaaaga acaattaata ttccctgaaa
     6841 tagaatatga taaaatagat aaagtaagag gaatggatat aatatttgtt actacagcaa
     6901 aaactgacga agaagctcgt gaattattaa aattattagg aatgccattt tctaagtaag
     6961 caaaggaggg attcccgtgg ctagaaaagc gatggttgtt aagcaacaaa gaaagcaaaa
     7021 gtacgctact agagaataca ctagatgtac tatatgcgga agaccgcatt cagttttgaa
     7081 aaaatttggt atatgccgta tatgctttag agaattagct tataaaggtc aaatacctgg
     7141 tgttagaaaa gcaagctggt agaagcttga ttagaatgga aggagggcaa caaaatgaca
     7201 atgacagatc caattgcaga tatgcttact cgtataagaa atgctaatgt tgttaagcat
     7261 gaaactgtag atgttccagc ttctaatatg aagaaagaat tagctagaat cttattagaa
     7321 gaaggtttca taagaggtta tgatgttata gaagatggaa aacaaggtat cataagaata
     7381 caattaaagt acggacaaga aggcgaaaga gttataacag gtcttaagaa aatatctaag
     7441 cctggaatga gagtttacgc tgctaaccat gagataccaa aagtattaaa cggattagga
     7501 atatcagtta tatctacttc aaaaggaata ttaactgaca agcaagctag aaaagaaaat
     7561 gttggtggag aagtaatctg ctacgtttgg taattaatat gaacaatgta aggaggtgcg
     7621 accatgtcaa gaataggtgt taagccaata ataataccag caggtgttga agttactata
     7681 gctgaaggaa atttagttac agtaaaaggt ccaaaaggaa ctttaactaa acaattaagt
     7741 gctgagttaa acataaaaaa agaagaaaat acaataatgg ttgaaagacc aactgataat
     7801 aaaaaacata gatctttaca cggactaact agaactttat tagataatat ggttgtaggt
     7861 gtaaatactg gtttcgaaaa gaaattagaa ttaaaaggtg ttggatatag agctcaaaaa
     7921 caaggaaaga aattagttat gaacttaggt ttctctcatc cagttgagat ggaagatcca
     7981 gaaggaataa ctgttgaagc tccaaaccaa acagagttaa tagtaaaagg tatagacaag
     8041 caattagtag ggaattatgc tgctaagata agagcttgga gaaagccaga gccatacaaa
     8101 ggtaaaggta taaaatacgt tgatgaagta atcagacgta aagaaggaaa aactggtaaa
     8161 aaataatacc aaaacttaaa cgctagaaag gagtgagcta ggtgttaaaa aaggctgata
     8221 aaaatgctaa tagacttcaa agacataaga gagttcgtag aaaaataagc ggtacttctc
     8281 aaagaccaag attatgtgta tttagaagtg ctaacaatat atatgctcaa ataatagatg
     8341 acacaaaaag agtaactcta gttgctgcat cttctttaga agcagaagtt aagagtgctg
     8401 tcaatcatac aggaaacaag gaagcagcta agaaagttgg agaattagtt gctaaaaaag
     8461 ctgttgaaaa aggaatcact gaagttgtat ttgacagagg tggatactta tatcatggaa
     8521 gaattcaaga attagctgaa ggtgctagag aagctggtct taagttctaa tacgaaggag
     8581 ggaaaaatac atgctacgtc gtaagccgat agatgcagga caacttgact tacaagaaaa
     8641 agttgttgaa gtaagacgtg ttactaaggt tgtaaaaggt ggtagaaatt tcagatttgc
     8701 agcattagta gttgttggag atgaaaacgg acacgttggt ataggtgctg ggaaagcaat
     8761 ggaagtacca gacgctataa aaaaagcagt tgaagatgct aagaaaaatc taatagtagt
     8821 gccaatagtt gggactacaa ttcctcacga agtacgcgga cactttggtg ctggaaatat
     8881 attaataatg cctgctgtag aaggtacagg agtaatagct gggggacctg ctagagccgt
     8941 acttgagcta gctggactaa aagacgttag agctaaatct ttagggtcta acaatccaag
     9001 aaatatggta aatgctacaa tagaaggact taattctcta aaaacagttg aagatatagc
     9061 taaacttaga ggtaaaaaag ttgaagaact tctagggtaa ggaggtagtg taaaatggct
     9121 aaattacaaa taaagttagt tagaagtgtt ataggaacta ctcctaacca aaaaaagaat
     9181 gttgaagcgt taggattaag aaaaagagaa caagttgttg taaaagaaga caatgctcaa
     9241 acaagaggta tgataaataa agtaagtcac ttattagaag taactgaaat agctgaataa
     9301 tttttataaa gtagtgcaag gaggtgcaat ccatgaagtt acatgagtta aaacctgctg
     9361 aaggtgcagt aagagctaag agaagattag gtagaggtac tgcaactgga caaggtaaaa
     9421 ctgcaggacg tggacaaaaa ggtcaatggt ctcgttctgg tggtggagta agagtaggat
     9481 ttgaaggtgg acaaatgcca ctagctagaa gacttcctaa gagaggtttc aataacatat
     9541 ttaagaaagt ttacacagaa gttaatgttg aagttttaaa cagatttgaa aatggaacag
     9601 aaataactgc agaattatta aaatctacta aaactataag caaaataggt aaagacggaa
     9661 ttaaaatctt aggtgaaggt aatttagaga aagctttaac tgttaaagct gctaaattta
     9721 cagcttcagc tcaagaaaaa atagaaaaag ctggtggaaa agcagagtta gtataataac
     9781 ctgctaatct cctagccagt acaggcgggg tgaattagcg tgctgtcaaa attaaaacaa
     9841 gcttggaaga ttaaagcagt aagaaaaaaa gtaatgtata cacttatgat gatcgtgata
     9901 ttcagaatag gtactactat accagttcca ggcatagaca ctagtattat acaaaagatg
     9961 gtaggcggca atagtctact ttctctgtat aatatgttta ctggtggagc atttagtaac
    10021 ttttcactat ttgctttagg tataagtcca tatatcactg catcaatcat aatacaactt
    10081 ctaacagtag gttttgaaag tttagcagaa cttcaaaaat ctggtgaaga aggaaagaaa
    10141 aaaatcaata agtacactaa atatacagca ctagctctag cagttgtaca agctttggga
    10201 attacattag gaattgtaag aagtgcttta atatcaaata gtgtgttttt tatcacaact
    10261 gttgtaataa cattagtttc agcaagtatg ttagtaatgt ggattgggga caaaattact
    10321 gaaaaaggaa tcggtaatgg tagttcagta ataatatttg caggtataat atcaagaata
    10381 cctacagatg ttattaaaat atcgcaacaa gttaaatctg gcgaagttgc accttgggtt
    10441 atagtgattc tagctgtagt tatattactt acagtaacag gagttacatt tatacaagaa
    10501 gcaactagaa agatacctgt acaatatgct aaaagagttg ttggaagaaa aatgtatgga
    10561 ggacaaagtt ctcatatacc aatgaaagtt aaccagtcag gagttatgcc tataattttt
    10621 gctagttcac ttttagcttt ccctcaaaca atagctatgt ttatgggtcc aaatgctcag
    10681 gcttttgttc aaaaatactt aagtatggca acagaacaag gattttggac atatagatca
    10741 attgagattc tacttataat tttcttctca tatttttata caactgtatc atttaataca
    10801 gaagacatat ctaaaaatat gaaaaataat ggtggattta taccaggtat aagaccaggt
    10861 gagcctacta tggattattt aaatagaatt ttaactagat taactttagc tggagctact
    10921 ttcttagcaa taattgctat ggtgccagct cttacaacac attatatgaa agtaaatatg
    10981 agtctagctg ggacttcatt gctaatcgtt gttggagttg ctttggagct taagagacag
    11041 ttagagtcaa acttagttat gagaagctat caaggtttct taaaataaaa tggagatgga
    11101 taatatgaga ataatattac ttggacctcc aggtgctggt aaaggtactc aggcggcagg
    11161 aatagtagaa aaatacaata tacctcatat atcaacagga gatatattca gaaagaatat
    11221 aaaagaggga acagaacttg gaaaaaaagc taaagaatac atggaccaag gtttattagt
    11281 accagatgag ttaactgtag gtttagttac tgatagaata tctcaagaag attgtaaaaa
    11341 tggatttatg ttagatggat ttccaagaaa tgtagcacaa ggagaacatt tagatatctt
    11401 cttaaaaaat gctggtatat cactagataa agttgtcaat attgaagttg ataagagtat
    11461 attagtgtct agagcagttg gtagaagaat atgtaagtct tgtggagcta cttaccatgt
    11521 tgagtttaat cctcctaaag tagaaggtgt atgtgatgta tgccaaggag aattatatca
    11581 aagagctgat gataatgaag aaactgtatc taagagaata caagtttatc tagatgaaac
    11641 taagccttta gtagattatt atagcaaaca aggtataata gcagatataa aaggtgatca
    11701 agcaatagat aaagtatttg aagatattgt cgcagcctta ggaagtggaa aataaatgat
    11761 tatcttgaaa tcaaaaaaag aaatagaact tttaagagaa gcaggtaaaa tagttgctga
    11821 cactcatgaa gttttaagaa aagctatttc tccgggaata tctactttag aattagataa
    11881 aatagctgaa gagaatataa gaaaatacaa tgcagagcct tcttttaaag gatatggagg
    11941 ttttccagga agtatatgtg cttctatcaa tagagaggta gtacatggta ttccaggaga
    12001 aactatatta caagaaggtg acattgtaag tttggatata ggagcctatt ataaaggata
    12061 tcatggtgat tctgcgaaaa ctcacggagt aggcatgata tctgaagaag ataggaaatt
    12121 gatagaagta actagagaaa gcttctatga aggaataaaa tttgctaaat taggatatag
    12181 actttcagat atctcacacg cagtacaaac acacgttgag aaacatggtt tctcggtagt
    12241 tagagattta gtgggtcatg gagttggagc aaatcttcat gaagacccac aagtacctaa
    12301 ttatggactc ccaggaaaag gaccaagact tagagaagga atggttattg ctatagagcc
    12361 aatggttaat gcaggtcgtt atcatgtaaa gactctatca gatgggtgga cgactgttac
    12421 aatagatgga aaaaaatcag ctcattatga gcatacgata gcaattactg aacatgaacc
    12481 tttgatattg acaaagttgt aatttaaaaa gtaggtgaaa aaagtgctat cagataattt
    12541 aagtataggt caagttgtta aagtttcatt aggacgagat aaaggaaatt tattctttgt
    12601 agtcaaaata attaataatg aatatgtatt aatagctgat ggaaaaaaga gaaaacttga
    12661 caaacctaag ttaaagaaag tgaagcactt aaaaaaatat aattttatta atgatgaagt
    12721 tagaaaaaga gtagtatctg gacaagagat tacagattct tttttaagag ctgaacttac
    12781 taagttaaat tagataattt aacttattga aatggagggc tggttagtta atggccaaaa
    12841 aagatgttat agaattagaa ggtacagttt cagaagcttt acctaatgct atgttcaaag
    12901 ttaaactaga aaatggacat gagatattat gccacatttc tggaaagcta agaatgaact
    12961 tcataagaat tcttgaaggt gacaaggtga atgttgaact ttctccatat gaccttacaa
    13021 gaggaagaat tacttggcgt aagaagtaga ctatattaat atttagtcta aggagggatt
    13081 aataatgaag gttagaccat cagtaaaacc aatatgtgaa aaatgtaaag taataaaaag
    13141 aaaaggaaaa gtaatggtta tctgtgaaaa tccaaagcat aagcaaaagc aaggataaga
    13201 aattattact agcattttgt taaattttat agtataatac tatattgtga tgttaacaaa
    13261 agaacgttaa aatcgttgaa caatctggcg taggattggc atatgatgtg aagttttaaa
    13321 cactaaaaac tatgtgtagg aggtgcgatc atggcaagaa tagctggggt agatttacct
    13381 agagaaaaaa gagcggagat aggcttaact tatatatatg gtataggtaa agcaactgct
    13441 aacgaaatat tagctaaggc tgagataaat ccagatacta gaataaaaga tttatcagaa
    13501 gatcaagtta atgagttaag aaaagttata gatgacgatt tcttagttga aggtgactta
    13561 agaagagaaa ttgctttaaa cataaagaga ttaagagata taaaatgtta tagaggaata
    13621 agacacgcta aaggtcttcc attaagagga caaagaacta agactaatgc tagaactaga
    13681 aaaggtccta gaaaaacagt atctcgtaaa aagaaaaaat aaataaataa taactagatg
    13741 aaggagggaa aaggaaaatg gctaaaccaa aaaagaaagt tacacgtatt agaagaagag
    13801 aacgtaaaaa tatagaacgt ggtcatgctc atatacaatc aactttcaat aatacaataa
    13861 taactttaac tgacgttcac ggaaatgcta tatcttgggc aagttctgga caattaggat
    13921 tcaaaggatc aagaaaatca actccatttg catctcaaat ggctgctgaa acagctgcaa
    13981 aagctgcgat ggaacacgga ctaaaaagtg ttgaggtatt cgtaaagggg ccaggttcag
    14041 gaagagaagc tgcaataaga gctttacaag caactggact agaagtaact atgataaaag
    14101 acgttactcc aatcccacat aacggatgca gaccaccaaa gagaagaaga gtgtaattaa
    14161 taagagtaca ataggaggtg tagataaatg gcaagatata caggtgcatc atgtagacaa
    14221 tgccgtagag agggaatgaa attattcctt aaaggtgata gatgttatac agacaaatgt
    14281 gctatagtaa aaagaaacta tgctccaggt caacatggcc aaggaagaaa gaaagtttct
    14341 aactatggat tacaattaag agaaaaacaa aaagttaaaa gaatatatgg agttttagag
    14401 actcaattta gaaatttata tgaacgtgct gaaaatatgc ctggtaaagc aggtgagaac
    14461 ttattaagtt tattagagag aagattagat aatgtagttt acagaatggg actagcatct
    14521 tctagaaaag aagctagaca attagtgact catggtcatt tcactttaaa tggaaataaa
    14581 gtagacatac cttcattaat agttaaggtt ggagatgtta tagaagttaa agagaaatca
    14641 agatcttctg caaaattcaa aaatttagta gaagttaact caagaatagc tcctaagtgg
    14701 ttagaagcta atgtagaagg aatgacagca aaagtagttg gtgttccaac aagagaagat
    14761 atagatcttg agatagcaga gcacttaatc atagaacttt actctaaata ataagcacac
    14821 ttttaaaaat tattttaaaa ttttgtttac cctcagtgga ttactgaatt ttaaggaggg
    14881 ttttgtccat gatagaaata gaaaagccaa aagtagatat agttgagctt agcgaagact
    14941 atagatatgg taagtttgtt atagagcctc tagaaagagg atatggaata actataggta
    15001 acgcattaag aagaatatta ttatcatcat taccaggtgt agcagtgaat gctataaaaa
    15061 tagatggagt tcttcatgaa ttttcaacaa tacctggtgt taaagaagat gttactgaga
    15121 taatattaac tttaaaagag ctttcagcaa ctatagatgg tgaaggaagt agaacactta
    15181 aaatagaagc tcaaggacca tgctctatca caggtgcaga tataatctgt cctccagatg
    15241 ttgaaatatt aagtaaagat ttagcaatag ctacattaga tgataatgct aaacttaata
    15301 tggagatatt tgtagataaa ggtagaggtt atgtttctgc tgaagaaaat aaaacagaga
    15361 atgttccaat aggtgtttta cctgtggatt caatatatac tcctgttgag aaggttagtt
    15421 atcatgtgga aaacacaaga gttggtcaaa aaacagatta tgataaatta gtactagaag
    15481 tttggactaa tggtagtata aatcctcaag agggaatatc attagctgca aaggtattag
    15541 ttgagcattt aaacttattt atagacttaa ctgaacatgt aagtagtgtt gaaatcatgg
    15601 tagaaaaaga agaagatcaa aaagaaaaag ttcttgaaat gactatagaa gaattagatt
    15661 tatcagttag atcatataac tgtttaaaga gagcgggaat taatacagtt gaagaattag
    15721 ctaataaatc tgaggatgat atgatgaagg ttagaaacct aggtaagaag tcattagaag
    15781 aggtaataca aaaactagaa gaacttggat tgggattaaa accaagtgaa gaatagcgag
    15841 caaaggaggg ataagaatgg ctaagtaccg taaattagga cgtgaaacag ctcacagaaa
    15901 ccttatgtta agaaacttag taacttgctt actaagaagt ggaagaatag aaactacagt
    15961 gactagagcg aaagaaactc gtagaatggc tgaaaagatg ataactcttg ctaagagagg
    16021 agatcttcat gctagaagac aagttttagc ttatgttatg gatgaaacag tagttaataa
    16081 cttattcaca gatttagctc caaaatatgc tgagagaaat ggtggataca ctagaataat
    16141 aaaaataggg ccaagaaaag gcgatgctgc tgaaatggct tttatagaat tagtatagta
    16201 aggaaaggat taggcttgat ggcttaatct tttttttatt tttttgacta aggtatgata
    16261 taataatgtg taaaatgatt tttataagtt attgcttact aaaaggagta tgagttaatg
    16321 gataatatag taaaggtaaa taatatttcg tttgagtata ttacagatga agcaaaactt
    16381 aaagcaatag ataatttaag cctagatgtt aaaaaaggag aatttgttgc gataatagga
    16441 cataatggtt ctggtaagtc tactttatct aaaaacttaa atgctattct tatgcctact
    16501 gaaggaaata ttcttattga tgatatggat actaaagaag aagaaagatt atgggatata
    16561 agacaaactg caggcatggt atttcaaaat ccagataatc aaatagttgc tactatagta
    16621 gaagaagacg ttgcatttgg acctgagaac ttaggtatag aaccaaaaga aataagacga
    16681 attgtagagg agtctttaaa aagtgtaggt atgtatgatt taagagatag acagcctcat
    16741 ttgttatcag gaggtcaaaa gcaaagagtt gcaatagcag gaattattgc tatgaggcct
    16801 aaatgtataa tatttgatga agcaacagca atgttagatc catctggaag aaaagaagtt
    16861 atgaaaacaa taaaaagact taataaggaa gaaaatataa ctgttataca tataacgcac
    16921 tttatggaag aagcagtaga agctgataga gttgtagtta tggaaaaagg aaagaaaatc
    16981 ttagagggaa ctcctagaga agtattcagt aaaataaaga tgttaaagga aatcggttta
    17041 gatgtacctt gtatgacaga gttatctagt ttattaatag aagaaggtat aaatattagt
    17101 agtgatatat taactgtgga tgagatggtg atggaattat gtcaattata gtaaagaatt
    17161 taacacatat atataatgaa gggatgcctt ttgcaagtaa ggcacttgat gatatttctt
    17221 ttgagattaa agatagagat tttgttggtc ttataggtca tacaggctca ggaaaatcta
    17281 cattaataca acatttaaat ggattactaa agccttcctc aggagaaata tttataaatg
    17341 attttaatat aacagataaa aacttgaatt taactgagat tagaaaaaga gtaggggttg
    17401 tatttcaata tccagaatac caattgtttg aagaaacgat tgataaagat atagcttttg
    17461 gaccctctaa tttaggctta gaggagtctg agatacataa cagggtaaaa gcatcaatgg
    17521 aagctgtagg acttgattat gagggattta aagacaaatc tccttttgaa ttatctgggg
    17581 gacaaaagcg tagagtagca attgctggag ttatagccat gaacccagaa gttctcatat
    17641 tggatgaacc aactgctggt cttgaccctg gtggaagaga tgaaattttt aacttaataa
    17701 aagacttaca tgaaaaaaag aatatgacta taatactatc atcacatagt atggatgata
    17761 tggcaaagtt agcaaaaact ttaattgtta tgaatcatgg tagcgtagaa tttatgggaa
    17821 ctcctagaga agtgtttaaa tcaaatgcta gtaaattaaa agatatagga ttagatattc
    17881 cacaagtttt agagcttgca ttgaaattga gagaaaaagg ctttgacatc agtgaggata
    17941 tattgacttt agaagaagca aaacaggaaa tattaaaagt tgtgagagga cgaggattat
    18001 gttaaaagat ataactatag ggcaatacta cccaacaagt tctgctattc ataaattaga
    18061 tccaaggata aagcttgtag cgacaattgt atttatggta tctatatttg tagtaaataa
    18121 attttggcct tatatagtag ttttattatg tttattagct atgataaaat tggctaatat
    18181 accagtcaaa tatattgtta agggtgttaa acctcttaaa tggattattt tgtttacatt
    18241 tcttataaat atttttttct taccaggtga tgaaatatgg tcatttggat ttttagctat
    18301 tacaaaacag ggtctaagac aagcaatttt tatggcaata agattaatat ttttagtagt
    18361 tggtacatca ttgcttactt taacaacttc accaatagaa ttaactgatg gaatagagag
    18421 attgttgaat ccatttaaga aaattggttt gccagttcat gaacttgcaa tgatgatgac
    18481 aattgcttta cgttttattc ctacattgtt agatgagaca gacaaaataa tgaaggctca
    18541 gatgtctaga ggtgcagatt ttgaaagtaa gaatcttata aatagagcaa agaatttagt
    18601 accacttttg gtacctttat ttgtaagtgc atttagaaga gctgatgaat tggctatggc
    18661 catggaggcc agatgttaca gaggtggaca taataggact aagatgagag agtctgttat
    18721 atcaagaagg gattatatgg catgtgtatt tcaggttgtt tatctgggag caattatagc
    18781 tactagattt atagcaattt aattaataag ttatagatat aatgaggtaa agtatgagaa
    18841 atataaaaat taaaatccaa tacaatggta agaattattg tggatggcaa aagcaacctg
    18901 attctttagg gatacaaggt acaattgaaa gggctatata tgatataaca aaagaagaaa
    18961 ccagtcttat agggtctggt agaactgatt caggagttca tgctattggt cagattgcaa
    19021 attttaaaat taattcaggt ataagtatag aaagtatacc aatggcacta aatgccaagt
    19081 tgccaaaaga catatcagtt atagaagcat gtgaagtaaa tgatgatttt cactccagat
    19141 acagtgcaaa aggtaaaaca tataaatatt tagtatataa tagcaagttt agaaatccaa
    19201 tacttagtga gatatcatat caagtaaaat atgaacttga ttttgataaa atgtgttcag
    19261 aagcaaaaag tttattagga acacatgatt ttaaaggttt tatgagttct ggttcatctg
    19321 taaaagatac agttagaacg atatatgata ttgatataag taaaaaagat gacttgatta
    19381 cttttgagat tagtggtaat ggttttttat ataacatggt gaggattatt gttggaacac
    19441 ttgttgatat gggtcgtggc agaattaacg aaccattttt agatataata caatcaaaaa
    19501 ctaggtcaag atgtggacat acagctccag cacaaggatt atttctcaaa aaagttcatt
    19561 attaacttga cacaccaggc tacatgtatt aaaatatatg agaatgtgtt aataagtcca
    19621 cttgccccgg acttgacata aacggtaaaa aaattttatg aaaagtagtt aaggagggac
    19681 agttatgaaa agttatatag ctaagccagc tgatgtacaa agaaaatggt acttagttga
    19741 tgctgaagga aaaacattag gtcgtttagc aacagaaatt gcgacagtat tgagaggtaa
    19801 acataaacca acatttactc ctcacgttga tggtggagat tttgttgttg tagtaaatgc
    19861 agaaaaaata gttttaagtg gtaagaaatt agatcaaaaa tattacagat accatactgg
    19921 ttatgtaggt ggattaaaag aaatatctta cagagatatg atggataaga aaccagaaga
    19981 agttatatca catgctgtaa gcggtatgtt acctaaaaat aaattaagaa gcagaatgat
    20041 gactagatta agagtatttg caggtgctga gcacactcat gcagctcaaa atccagaagt
    20101 tttaaacttt aaataagatt aataggtgaa aggaggagtt tataatggct aacgttcaat
    20161 attatggaac tggaagaaga aaaagttctg ttgctagagt aagactagtt gcaggtgaag
    20221 gaaatatatt agtaaatgga agagcattag aaaattattt taattatgag acattaataa
    20281 gagatgttaa acaaccatta gttttaactg gtaatgaaaa taaatatgat gttatagtaa
    20341 aagttgaagg tggaggattc actggacaag ctggagctat aagacatgga atatctagag
    20401 ctttattaaa agctgattta gatttaagac ctgctttaaa gaaagaaggt ttcttaacta
    20461 gagatgcaag aatgaaggaa agaaagaaat acggattaaa agcagcaaga agagctccac
    20521 aattctcaaa aagataatta cttatttttt tggaaaagca aaatccttta tttactgtta
    20581 ttcagaaagt attggtattt taaaaatgtt gggttaagtt aattattgat atataataaa
    20641 ataataagat atcttattag gatgtttttc attaagaaga acatcctttt tattttgact
    20701 atttaataaa aaacatatgc taataacatt taaattttat ttttataata tt
//
LOCUS       DACTGW010000050        20100 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.050, whole genome shotgun sequence.
ACCESSION   DACTGW010000050 DACTGW010000000
VERSION     DACTGW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20100
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.050"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..224
                     /locus_tag="I9573_001458"
     CDS             <1..224
                     /locus_tag="I9573_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HAT4796084.1"
                     /translation="SIIILKIGEEGCIMASSSSSMNKIKDLTKEEILRIENDIKERNF
                     KIQDFKMERIKKKYDFSRYIGKFTKKEDK"
     gene            225..890
                     /gene="fliP"
                     /locus_tag="I9573_001459"
     CDS             225..890
                     /gene="fliP"
                     /locus_tag="I9573_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAT4796085.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            903..1172
                     /gene="fliQ"
                     /locus_tag="I9573_001460"
     CDS             903..1172
                     /gene="fliQ"
                     /locus_tag="I9573_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAT4796086.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            1184..2998
                     /locus_tag="I9573_001461"
     CDS             1184..2998
                     /locus_tag="I9573_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HAT4796087.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTARMIFFSMDGHHVLIRGIRYSFELIPIGKSFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVLKR"
     gene            3007..5082
                     /gene="flhA"
                     /locus_tag="I9573_001462"
     CDS             3007..5082
                     /gene="flhA"
                     /locus_tag="I9573_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAT4796088.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVSKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAEIKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            5057..6073
                     /gene="flhF"
                     /locus_tag="I9573_001463"
     CDS             5057..6073
                     /gene="flhF"
                     /locus_tag="I9573_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HAT4796089.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVVNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            6070..6948
                     /locus_tag="I9573_001464"
     CDS             6070..6948
                     /locus_tag="I9573_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HAT4796090.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            6962..7663
                     /locus_tag="I9573_001465"
     CDS             6962..7663
                     /locus_tag="I9573_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HAT4796091.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELNVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            7679..8101
                     /locus_tag="I9573_001466"
     CDS             7679..8101
                     /locus_tag="I9573_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HAT4796092.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            8086..8271
                     /locus_tag="I9573_001467"
     CDS             8086..8271
                     /locus_tag="I9573_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HAT4796093.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            8312..9085
                     /locus_tag="I9573_001468"
     CDS             8312..9085
                     /locus_tag="I9573_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HAT4796094.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNDYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            9106..9867
                     /gene="flgG"
                     /locus_tag="I9573_001469"
     CDS             9106..9867
                     /gene="flgG"
                     /locus_tag="I9573_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HAT4796095.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRIYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNIDGLGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            9890..10771
                     /locus_tag="I9573_001470"
     CDS             9890..10771
                     /locus_tag="I9573_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAT4796096.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMIVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVIKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            10774..11148
                     /locus_tag="I9573_001471"
     CDS             10774..11148
                     /locus_tag="I9573_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAT4796097.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELNTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            11180..12583
                     /locus_tag="I9573_001472"
     CDS             11180..12583
                     /locus_tag="I9573_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAT4796098.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNVKVDDYKNEFLHKSSSNHKIND
                     YNNVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEADVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            12699..14636
                     /gene="htpG"
                     /locus_tag="I9573_001473"
     CDS             12699..14636
                     /gene="htpG"
                     /locus_tag="I9573_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAT4796099.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITIQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            15024..16154
                     /locus_tag="I9573_001474"
     CDS             15024..16154
                     /locus_tag="I9573_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAT4796100.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDIDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            16381..17403
                     /gene="splB"
                     /locus_tag="I9573_001475"
     CDS             16381..17403
                     /gene="splB"
                     /locus_tag="I9573_001475"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002580203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HAT4796101.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            17585..18496
                     /locus_tag="I9573_001476"
     CDS             17585..18496
                     /locus_tag="I9573_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT4796102.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYNTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            18511..18927
                     /locus_tag="I9573_001477"
     CDS             18511..18927
                     /locus_tag="I9573_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HAT4796103.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(19021..19413)
                     /locus_tag="I9573_001478"
     CDS             complement(19021..19413)
                     /locus_tag="I9573_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4796104.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            19554..19949
                     /locus_tag="I9573_001479"
     CDS             19554..19949
                     /locus_tag="I9573_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5-phosphate oxidase"
                     /protein_id="HAT4796105.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT         8099 a         2143 c         3578 g         6280 t
ORIGIN      
        1 atagcataat aattttaaaa ataggtgaag aaggatgcat tatggcatct tcttcctcta
       61 gcatgaataa aataaaagat ttgaccaaag aagaaatatt aagaatagaa aacgacatta
      121 aagaaagaaa ttttaaaatt caagatttta aaatggaaag aattaaaaag aaatatgatt
      181 tcagtagata tattggaaaa tttacaaaaa aggaagataa gtaaatggaa ctattatcaa
      241 aattaccttt ggctgatgta ccatatacaa gttcaatgca attgttttta tttctaactg
      301 cattaatgtt tttaccattt gtaatgttta tgatgacaag ctttgtcaga atagttataa
      361 gtttatcatt tttgaaatca gcattaggag cacagcaagc aattccaagc caaattttgg
      421 tagggcttgc aatagtattg acaatattta taatgagacc agtattaaat gagataaacg
      481 aaaaggcatt acaaccatat atgaaagaag aagttactat ggaagaagct atgaaggaag
      541 cagagggacc tattaaagaa tttttattaa agcaaaccag acagacagat ttagatttat
      601 ttgtagaaca ggctggtttg aaagaaaaga aattaactcg tgaaaatata ccactatctg
      661 ttgtagttcc agcatttgct ataagtgaac taaaaactgc ttttcaaata ggttttttaa
      721 tatatatacc atttttgata atagaccttg ttgtggcaag tgttttgatg tctatgggta
      781 tgtttatgtt gcctccagtc atgatatcat taccgtttaa gttattatta tttgtaatgg
      841 tggatggatg gaacttgata gtaaaaacat tgatattggg atttggatag gagaaaaata
      901 gtatgagtga atttatgata atgagtgttc ttaaaaatgg catgtttaca ggagctatga
      961 ttgcagctcc agtactcatt gtttctcttg tagttgggct tttaataagt gtgtttcaag
     1021 ctacaactca aatccaagag caaactttga cttttgtacc aaaactaata gcaattcttt
     1081 tagtagtagc tattggtgga agctggatgt tacatactct tattagcttt acttcggaga
     1141 tattcaatat gatgtctctt ataactaaat aagtaggata tatatgattt ttgtatttat
     1201 tagaatagtg gcattctttg gaacactaag gatactattt ccagctggta cacctgcttt
     1261 atttaagtcg ctctttgcaa tcattatatc aatattgatt tcaagtacta tgcaaattga
     1321 atatgcaaca aatatagaca acattgtatt gtttacctta tatggtataa atgagaccat
     1381 aactggcatt atgcttggat atataaccaa tctatgtttt tatagtatca gaatggcagg
     1441 ttctatgatg gatcaacaga tgggattatc aatgattaat atgtttgacc caaattcaat
     1501 gactcagaca actttaattg ataatcttat gaactggaca gcacgtatga tattttttag
     1561 catggatggt catcatgttt taattagagg cataaggtat agttttgagt taattccaat
     1621 agggaaatca ttcgtagata ataatataga ttatataata aacatatttg tgcagtgctt
     1681 tttaactgga ttcaaaatag caatacctat tgtactttgt ctgcttatgg cagattttat
     1741 attagggctt atatcaagaa gtgtacctca actaaatgta atgataattg gtatgccatt
     1801 aaaaatactg gttggtattg cgttatttat catatcaata cctttaattg tcaatcaaat
     1861 aagtcattta ttaagtcaaa taccaaagat gtatgaggga actttcgcac tagctcctat
     1921 gttttttatg ggttcaacag ataaaacaga agaggctact ccaaagaaaa agggagaaca
     1981 aagaaaaaaa ggtaatatag caaagagtaa agaattaccc gttgcaatga ctctacttgc
     2041 attcacttta ttagtaccta cattattttc atatgtagtg gatacactta aatcttctct
     2101 taattatttt ttgagtctag atttttatat gaatataaat tattcaagta tagagaaatt
     2161 agttatagca ggattaatgg atttttttaa gatattttta ccaatagcta ttccattctt
     2221 agttttaggt atagtcgcaa acctatttca agtgggaata ctgtttactg gtgaaacttt
     2281 aaaacccaat ttatcaaaat taaatccagt tagtggattt aaaaacatgt tttctatgag
     2341 aagtttgtca accttaataa aggatacagc gattatatct attttggctt acataggata
     2401 tacattcttc caagataatt atttagatat attaaagcta ggtaacatat acctgccaac
     2461 tttaatgtac acagttaaag atttagtata ttcaatatta agtaagattt gtgtagctat
     2521 gatagttata gcagttgcag attatgttta tcaaagatac agtcataaaa agcaacttag
     2581 aatgacaaaa caagaagtta aagatgaata taaaaactca gaaggagacc cagaaataaa
     2641 agctaagata aaacaaaaac aaagacaaat ttcgtctcaa agaaccatgc aagcagtacc
     2701 aagtgctact gtaatagtaa ccaatcccac ccacttatct atagcagtga gatatgaaaa
     2761 agggaaagac caagctccag tagtggtggc aaaaggtgca gattatttag cttttaaaat
     2821 aagggaaatt gcaaagggaa atgacatacc tataatagaa aataaaccta ttgctaggtt
     2881 gttatataag caagtagaaa tagaccaaga aattccagag gacatgtatc aagcttttgc
     2941 agagatatta gtagcagtat ataaaatcaa aaatagatac aaagtcctca aaaggtagga
     3001 taagtaatga ataagtttaa tttaaaggaa aatatggacg taatagtggc atttggtgtt
     3061 gtagggattg ttcttatgat gatagtgccg ttacctacat ttatattaga tattttactg
     3121 gcaattaaca taagtttatc tgtgctaatt ttactaatga cattatttac aacaaatgta
     3181 cttgatttat ctatatttcc aacagtgtta cttgtgacaa cactatttag attgggatta
     3241 aatctttcat caactagatt gattttaaca cagggatatg caggtgaagt tatagaggca
     3301 tttggaagtt ttgttgtagg tggaaattac gtagtaggaa taattatatt cttaattata
     3361 ttaattgttc aatttgttgt tataaccaat ggttctggaa gagtatctga agtaacagct
     3421 agatttacgt tagatgccat gccaggtaag cagatgagta tagatgcaga tttaaatgca
     3481 ggagttatag atgacaaaac tgctagaaaa agaagaaaag agcttcaaca agaagcagat
     3541 ttttatggct ctatggatgg agcaaataag tttgtaaagg gagatgccat agcaggtctt
     3601 atagtaacag caataaacat aataggtgga attgtaatag gagctgtaat gctgaatatg
     3661 actcttatgg gtgcagcaca gacatatgta agacttacta taggggatgg tcttgtaagc
     3721 cagatacctg ctctattaat atctacatca gcgggtataa tcgtaacaag agcttctact
     3781 gatgaaaact ttggtggatt agtaagtaaa cagttaacgg caataccaaa agcagtaatg
     3841 atgactggtt gtgttctaat cgtacttggt atgatgcctg gattacctaa accagcattt
     3901 ttttcactag gtataggtgc tatagcagca ggttactttt taggaagaga aaagaaagat
     3961 actttagaag acttggagtt tgaaaaacct gatatggtaa atccagataa aaatgaagta
     4021 gaagatgtaa caaacttaat aaatgtagaa tcaatagaag tagaaattgg atatggttta
     4081 atatctttag ctgatgaatc agcaggagga gatttacttc agagaatagc atcaataaga
     4141 agacaatgtg caatagacat gggtatagtc gtgcaaccca taagaataag agataattta
     4201 cagttaaatc caaaccaata caatataaaa ataaagggaa atgtagttgg tgggtatgag
     4261 ataatgccta atatggtttt atgtatggac cctatgaatc aaggatttag tatatttgga
     4321 ataaagacaa ttgagcctac ttttggaata gaggcacttt ggatagaaag tgacaaggta
     4381 gaagatgcag agataaaggg ttatacagtt gtagattcat ccacaatact tataactcac
     4441 ttactagata taataaaatc aaatgcacac gaattactgg gaagacaaga agttaagact
     4501 attatagatg cagcaagaga aaattatagt gctgtagtag atgaattgat accagattta
     4561 atgacacttg gtgaaattca aaaagtactt aaaaatctgt taagagaaaa agtaaatata
     4621 aaggataggg ttacaatact tgaaacgctt gcagaccagt ctagaaatac aaaagatata
     4681 gagcttctta ctgaatatgt aagaatagcc ttggctagaa gtatttgtaa taatttagtt
     4741 gatgatgata agacaattgt agtggctaca ttgtctccag aaactgaaga gttggtatct
     4801 aataatttac aaaggtctgt aaatggaact tatccagcag ttgacccaga aaatacaaat
     4861 aaaatatttg agagtataca agaagtaatg aacaatgtat atttcaatca taatatacca
     4921 gtaatagtag tatcaccaaa gataagagct ccatttagaa aattagttga aattgtatat
     4981 cctaatttga ctgtattatc attaaatgaa atacctaacg atattaaaat caaagcagaa
     5041 ggtgtggtga gcatatatga tgacgaaaaa gtatacagct aacacaattc aggatgctat
     5101 gaatctagcc aaaatggaat tgggtgacaa tattacatta atagataaaa aagaagtaag
     5161 aaagtctgga atacaaggta tcttttcaaa gaaagatatt gaactaacta ttggatggga
     5221 aaagagagaa aacgtagaac aaaaagattt aaaacgagag atagaacaat taaagttaat
     5281 tattaacaat atggggtttg ataataagaa tgataatgat atagataaaa tatgtaaaaa
     5341 tctactaagt ttagaattaa atgaagaaat tgtagaatct ataagaattg atttgcaaga
     5401 aatgaagttc aatggaattg atacaagcaa aaatttagta gaaattttaa agaaaaagat
     5461 taaaatagaa aatcaagcaa taaatggtaa aattgctcta gtcggaccac caggagtagg
     5521 taaaacgact acaatagcaa agttagccgc aaaattagtt tttgaagaaa ataaaaaagt
     5581 aggagtaata actatagata catacaggat tggtgctgta gaacagttga agatatatac
     5641 tgatataatg aatattcctt ttaaaggggt aataagtccc gatgaaatgg aattggcact
     5701 tgatgagatg aaagattgtg atgttgtatt aatagataca acaggtagag gctataaaaa
     5761 ctctatgcaa atactagaaa tcaaaaatct tatagataaa gctgaaactg ataatataca
     5821 tttagttgtg aattgtacaa caagagaaag tgatacaaaa gcaattatag attcatatag
     5881 aaatgtgaat tttaaaagct taataattac aaagctagat gaaacaataa catatggctc
     5941 aatatttaac ataatgaatt atgcacaaaa accaatatca tatattacaa caggacaaaa
     6001 tgttcctgat gatattataa aacctaatga agataagatt ataaggttac tattaggagt
     6061 tgaaagtata tgatagacca agctcaaatt ttgagaaaaa tggcaatgga aaaaagagga
     6121 cttgatgagt ttatagtaga gaatgagaat gtacctaaga taattacaat agcttctggt
     6181 aaaggcggtg ttggaaagag taatctcgct acaaatttgt ctatatgctt gaccaaactt
     6241 aataaaaaag ttcttatatt agatgcagac ataggaatgt ctaatataga tataataatg
     6301 ggggtaaatg taaaagggac tataattgat gtaattaatg gcgaaaaaaa tatagaagat
     6361 ataatttcac agactaagta tggtgtaaat ataatatctg gaggttctgc ccttaatcat
     6421 atagaagact ttacagaagc tcaaagaaat aagtttatac atagcataga acagattcac
     6481 gatgtggatt ttataataat tgatacagga gctggtatga gcaaaagcct attgtcattt
     6541 atatactgta gtactgaatt tttcttaata acaactccag aaccgacatc attgactgac
     6601 gcatatagtt tattaaaagc tataagtaat tttggcataa agagaagtgc aaatataata
     6661 atcaataggg taattgattt agaagaagca aaatctacat acaaaagaat aaaaatggtt
     6721 gtggacaaat ttctaaacct taatctagaa ttgtatggat atataataga tgacaaaaaa
     6781 gtaagtttat gtgtaaaaaa acagatacca ttcattttag aatatcctac aagtattgca
     6841 tcaaaatgta ttatagccat agcaaagaga atggtaagtc agtcaagaga agatattgtt
     6901 gataataaaa gttttctaag aaagatgttt agtatattta aaagctaagg aggcgtagtt
     6961 aatgaataga gaagaattaa taaaagagaa tatgcctctt gtaaagagta tagcaagaaa
     7021 attttttata ccagggaaag gttttgaata tcaagactta gtaaatagtg gagtcattgg
     7081 attgattgat gcaataaata aatttgatgc tgaaaagggg gcaaagtttt ctagttactc
     7141 ttatataaaa ataaaatctg ctatattaga tgaaattaga aatcaaagtc caatttctaa
     7201 acataatcta actaaagtaa ataagtataa tagagtggta gaaaagttgc aatctaaatt
     7261 attaagagag ccaacttcat atgaaattgc aaaagaatta aatgtttcag aaaaagaact
     7321 tcatgatatc gaaagtaata tagacatgtt aaatattgta tcattaaact atgtaatctt
     7381 tgaagataca aatgaaactg tacaagatgt aataagtgat agagaagagg aagctccaga
     7441 aaatataata gaagaagaag aaaagttaga gattctatct aaagcaatta gtaatttaaa
     7501 tgaaagagaa aaattaatac tttcactgta ctattatgaa gatttaaatc taaaggagat
     7561 agggaaagtg ctaggtgttt cagaatcaag agtttcacaa ctccacagaa aatcaataag
     7621 aaacttaaga aacaagataa aagaacttaa atattctata tagatggtga ttgataagat
     7681 gatacaaatt atattaatag tagtgtctat tttaataata gtaactatag ttataaattt
     7741 acataaggaa tcaaaaaata aagataacag atactttgat aaaatatttg agcaagaata
     7801 tgaagcaaat gatagattaa taattgcaga aaaaagaaga ttctttttag acaaagtact
     7861 tgctgaaata agaagtaata atcatataaa agtttatgaa aattataacc taataaaggc
     7921 taatgaagaa agaaaggaaa acatatttat agaatcaaaa aaggaagtaa gacaagaaaa
     7981 tgagttatct cttaaagtga aacatcttat aagttcaggt tatgatgatg tggaaatatg
     8041 taagatgcta gatattggaa agggggaact gtcattaata aggagttgtt acaaaatata
     8101 aggatattat tatgtgataa aaaagtattc ttaggaatag gtattggtat attaatatca
     8161 actttatgca taatgctttt tagtaataat gatatgagta aggcagagat agaaaaaaag
     8221 gccagagcgt taggcatgaa atatacaagt gagatgaaag ttttagaata gatgatactg
     8281 tttagacatt tacaaaaaag gaggtcataa tgtgataaga ggattatata cagcagtttc
     8341 tgctatgata actaatcaac aaaagcaaaa tgttgttgtc aataacctag ctaatatgga
     8401 tacaaatgga tataaaagta aacaactttt gactaaaagt tttgatgaat taaagttgga
     8461 gggttacaat aactacgcaa atggaactaa acaaaagcag attataggcg atataagtcc
     8521 aggtatttct atggatgaaa ctatcacaaa ttttaatcaa ggacctataa aaactactga
     8581 taataaaatg gatgtagcta ttcaaggtaa gggatttttt ctagttagtg atgcagctgg
     8641 aaatcaattt tacactagaa atgggaactt cagaacagac aatcaaggtg accttataac
     8701 aagtgaaggg tattatgtac ttgggacgaa tactgctact ggtgctgtag gtcatataaa
     8761 tgtaggaaat caaaaatttg aagtggcaaa agataataca atatcactag atggaaatgc
     8821 tatgtataaa ttcaatattg tagattttaa tgactacaat aacttaaaaa aagaaggaga
     8881 taacttgtat tctggaggag gagctatgaa agtaaataat gcacttacaa tacaaagtgc
     8941 gatagagtct tctaatgtag atatggtatc tgaaattaat aatatgatga ccatatcaag
     9001 agaatatgag gcaaatcaaa agataattca ggctatggat tctaaattag caaaaatagc
     9061 aagtgagatt ggttcagtaa gataggtgat aggaggtgaa tttttatgta cacaatgttt
     9121 tatactagta aaacagcttt atcagcaaat caaagtaaac tagatataat atctaacaat
     9181 atagcaaatt ctgattcaac aggatataaa aaaataaata tggaatattc tgatttagtg
     9241 gatgaagtac taaatagacc atcatatcct acaaatggca atgatatatc gactggaaca
     9301 ggagctaagg caacagctcc catgagaatt tatgaacaag gtgcattggc ccctacagat
     9361 agtaagagta atttggcgat agatggagaa ggattcttta gagtgataag aaaagatggt
     9421 acatatgcat atacaagaaa tggtggattt aatatagatg gtttaggtaa gattgtagat
     9481 gataatggaa atatattaga tgttcaattt gatgcaggat ataactataa taatacgaat
     9541 atagatagca caaacttaac agtttcaaga aatggcgagt tattcgtaga taataaaaaa
     9601 ataggaaaaa tcaatttata tcaacctata ggaactcaaa attttatatc tgaaggagat
     9661 agtttattcg tcgcaactga tgcagcacag attaaacaag ttgagaaagt agatatagtt
     9721 caaggctata gagagaggtc aaatgtttct ttacaagaag aatttgttga cttaatagcc
     9781 actcaaagag catttcaaat gaactcaaag ggaataaaga cagcagatga aatgtggcaa
     9841 atagctaata atttaagagc taaataatta tgtaaggtga ggaatattca tgaatgatat
     9901 gaaagagtac gattttggca aacctcagag ttattcaaga gaaaatttaa cttgtttaaa
     9961 ttttttatta gctgaatttt gtaaaaaata taaaaactat ataatttatg aacttaagtg
    10021 taattctagt atgattgttg atagtataga ccaagtaaat tatcagactt ttttagatag
    10081 agtaacttct tcatgtgtag tagtacaaaa tgcaatggaa cctgttatga aaaacttttc
    10141 ttttagaata gatagaagtg tagctgatat gtggattgat ataacttctg gaggaactgg
    10201 agttataaag gataatgata gagagcttac agaacttgat aaaaaagtgc ttttacatct
    10261 tatggatgat ttagtaagaa atatgcactt atttgaaggt tttgaaaata tacaagtatt
    10321 ggatacacat acacatatta atttaccaca attatgttct ccaactgcac cagtatgcgt
    10381 tgtagattta aaagtgctaa atgataatga gtatattgga agtgtttctc tttgttttcc
    10441 gtataatggt ttagaggctc tattagaaag tatatctgtt atagaatttt ttgataatga
    10501 tgtaggagat gattcagagg aatttaccaa aaaaatctat aatagtgtat ctaatataga
    10561 attatcagtt attgcagaga taggaaaagt atcaattaat gttgaagact tacttaaact
    10621 agaagttgga gatgtaatag taacaaataa aaaaataaat gattatatag atatatttgt
    10681 agaaaattca aaatcatata cagcaacacc aggatttata tcaagcaaaa aaggtgtaaa
    10741 aataaatgat gctgttggaa aagaggtgta gttatggaca atagtaatct tatttcagat
    10801 gaagaagcaa agatgttatt aaatgataaa gttgaaagtg aagttgatgg ggatgatgag
    10861 ttagccttag atgtctctga agttaatcga aataatatac aaaggatact tgaccttaag
    10921 ttagaattat ctgtagttat agggagaact aagatgactc taagagatat tttaaattta
    10981 cataaaggtt ctcttattga actgaatact ttagcagacc aagatgtgga aatactaata
    11041 gataataagg ttttggctta tggaaaagta gtggtggtag atttaaattt tggtgtaaag
    11101 ataacaagta tagtaagtga agaagacatg gtaaaatctc ttgtttagag ataacatcaa
    11161 ttaatactta aggcatgagg tggtacttat tttaaactta aatgatataa aaaaagatat
    11221 actaaaactc aataaaaaag actcatgttc tctatataat gaatctatta ataatataaa
    11281 taataattgt atatcaactg gattaaaatt tttaaaacaa gcaagagatt taaacccaga
    11341 tgatgttgat atattaaatc tcacaggtct tgtaaactta ctaaaatgta attttgatga
    11401 tgctgtagaa agtttctata agagttcact ttgtgaaaaa actgctctgt gtgaaaagta
    11461 tataaacatg cttacatctg aggagttttt aattttcttt gaaaagtaca atcaacttat
    11521 aaggttaata aataaaggaa tgtataaaga agctatacaa gggtttaaac ttataactga
    11581 ccaatactgt gatttaatag aaccttatga gttgcttgcc ttactgtatg acaaggaaga
    11641 agaatatata aaatttgatg aatgtttaga agtattgaag attattgata aggaaaatta
    11701 tttattaaat caagaaaata acacattaga tatgtataat agtgctaata tacaaactaa
    11761 aagtaatgta aaagtagatg attataagaa tgagttttta cataaaagta gtagtaacca
    11821 taaaattaat gattataata atgtaactaa agaaaaaagt tcaagcaaaa accagaaaag
    11881 ttcagattct aataagaaaa aaagtattaa aaaaaataag tacctatata tagccataat
    11941 attaggaata ttattagtag gacaaagtat atatagttct tataagatga ataatttaac
    12001 tgataaaata tctaaaaata atgaaactaa aaatgaagag tcaatagatt ctaacttgac
    12061 taagaaagaa aataatatag aagaaaatga agatatgagt aaggcttcta atataaataa
    12121 gagtaataaa aacattaata aagaggctga tgtaaaatct aaaaaaaata ctgtagaaaa
    12181 tgaagatgat aaagattctg acttatttac agaggatgaa ttgatgtcta aggcgaatac
    12241 tttaaaatca gaaaaaaaaa ctaaatcttc tatacaacta tataagaaag ttgcagatat
    12301 aggtgaaaat aaagggaata catctgaagc aacttatcaa gttgctattc tcagtgaaaa
    12361 gttaaaagac tataaaactg ctgaagaata ttataaaatg tatgtagaaa attactcaga
    12421 aaaagatgct tattttgatg aatcttatta taatttaggg atgatgtact ataacaatgg
    12481 tgatttaaaa aattctaaat tgacattaaa gaagttagtt aataaggtac caaatagtat
    12541 gtataataat tcaaaagtaa aagaaatttt aaaagaagaa tagttaaatt agataagaaa
    12601 aataaattat tttattttaa aataatatga ttataatata ttgacatacc attttaaaaa
    12661 atttaaaata ttaattataa aatgaggagg gataaaaaat ggaatttgaa aaagggagta
    12721 tttcaattca tacagaaaat atttttccta taattaaaaa atggttatac tctgataaag
    12781 acatatttat aagagagctt ataagtaatg gatgtgatgc agtaagcaaa cacaaaagac
    12841 ttgtatcact aggagaaata tcagaaaata agtcatcaga ttataaaatt acagtatctg
    12901 taaataaagg agaaggtaca cttaaattta ttgacaatgg aattggtatg actgaagaag
    12961 agataaaaaa atacataaat caagttgctt tttcaggtgc tgaagatttc tttaataaat
    13021 ataaggataa gatggaagag tcaaatgaca taataggtca ttttggatta ggattttatt
    13081 ctgcatttat ggtgtctaaa aaggtacaaa tagatacact ttcatataca gaaggagcga
    13141 ctcctgtaag atggatatct gaaggtggta cagaatacga aatatctgaa tcagatgcta
    13201 gaaatgatag aggaactact ataacactat ttattgatga tgatagtaaa gaatttttag
    13261 atgaatttac tgtaagagga ataataaata aatactgttc tttcttacca gtagaaatat
    13321 atttagaaga tgttgaaaga ttagaaagag aagctaaaga agcagaagaa aaagctaaaa
    13381 aacaaaagga agatggaaaa gaagagattg tagatgcaaa agtaattgag cctttaaacg
    13441 acactaatcc tctatggtta aaatctccaa aagactgtac tgatgaggag tataaagagt
    13501 tttatagaaa ggtattcaat gtatttgatg aacctttatt ttggatacat ttaaatgtag
    13561 attatccttt caatttaaag ggtatacttt acttccctaa attaaaaaat gagtttgaac
    13621 tgactgaagg aaaagtaaaa ttatacaaca atcaagtgtt tgtggctgac aatataaaag
    13681 aagttatacc agaattttta cttttattaa aaggtgttat agactgccca gacttaccat
    13741 taaatgtatc aagaagtttc ttacaaaatg acagggatgt aagcaaaatt tctaaacata
    13801 taattaaaaa agtagctgat aagttaaaat ctctttgtaa aaatgaaaga gaagaataca
    13861 ataagttttg ggatgatata caaatattta ttaaatatgg ttgcttaaaa gatgaaagtt
    13921 tctatgagaa ggtaaaagaa tgtatattat tcaaaactat tgatgatgag tatataacaa
    13981 tacaagatta tttagaaaaa tgtaaggaca aacatgaaaa taaggtattt tatgtaagtg
    14041 ataaggaaca gcaatctcaa tacattaaac tatttaaaga atacgactta agtgctgttg
    14101 ttttaaactc atcaatagat actcatttta tttcatttat ggaatataaa gagaatggag
    14161 ttaagtttaa tagaatagat gcagaccttt ctgatgtatt aaaagataaa aatgaaaata
    14221 aagatagtga agaaaacaaa gaagagatag ctaagataga aggtttgttt aaagaagctg
    14281 ttggagaaag agtgaagaat tattctgtag aaggattgaa aaatgaagac actccagcta
    14341 tggtattagt atcagaacaa tctattagaa tggcagaaat gcagtcaaga tttgctggaa
    14401 tggatttagg aatgaatttt gaggaagaaa aaactcttgt aataaatgag aacagtccaa
    14461 taatcaaaaa attggtttct cttaaagatg atgaggaaaa gaaagataag attacgctta
    14521 tttgtaacca gatagcagat ttagctttac tttctaataa agaattaaag ccagatgagt
    14581 tagattcatt tgtacaaaga agtaataagc ttatgagtct tttgatagaa ttataagatt
    14641 gaatttgaat acattgaagc aaatttataa aaagtattca atatcagtga tgagaaagaa
    14701 gtgtctcaaa atgaactttt cagttcatga ggcgcttttt ttatgtgaaa ttaaatttat
    14761 tttaatcatt tcaacaatga aaaatatatt tatctctatt ttgagacaga ccgttcttta
    14821 tttttttatc tttggttaat ttttaaatat ttaagtaggt tctaataaaa aactctattt
    14881 agttaaatat agttaacttg atatataata agataataca tataattaag taaaagattg
    14941 ttttttagtt tatgtttaaa tacataatta cactttgtta aaaacataaa attattacta
    15001 aagtagaaat aggaggtttt agcatgaatt ttaattataa tttgccagta aatatcttat
    15061 ttgggagagg cagaattaat gatgtgggaa aagaagtatc taaatatggc aaaaaagtat
    15121 tgatagtaac aggtaaaaat agtacaaaga aaactggatt acttgataaa acaatagatt
    15181 tactaaaaga ctctaaaata gaatatgaag tttttgatag agtagaacaa aatcctctta
    15241 taactactat atattcaggc gtagaaatta taaaatcaac tggatgtgat tgtgttttag
    15301 ggcttggtgg tggaagcatt atggatgctg ccaagagtat agctttttct tataaaaacc
    15361 caggagattt aaatgattat atatttggaa ttagacaagg tgaagaagct ttacctataa
    15421 tcttagttcc aacaacatca ggtacaggta gtgaaggaaa ttgttttgca gtacttacaa
    15481 attcagaaac taaagataaa aaatctataa agaagaatag tatgtatgca aaagcatcaa
    15541 taattgaccc agaacttatg gttacaatgc caaagcatat aattgcatca gttggatttg
    15601 atgcacttgc ccataatatg gaagcatatc tttctaatgg aagaaaccct cttgcagatg
    15661 tacaagctat ttatgggatt gaacttatat cagaaaacct aataaaggta tataatgatg
    15721 ttaatgattt agaagcatgg gaaaaaataa cacttgcaag tactattgga ggaatgacga
    15781 ttggtacagc gggtacagcc ttgccacatg gaatggagca tcctgtaagt ggtcttagag
    15841 atgtaatgca tggaaaagga cttgcagcgt tgactcctac tattatagaa agttcatggg
    15901 atattgatat tgaaagattt ggtaatattt caaaggtatt aggaggaact tgtgcagaag
    15961 attgttcaga tgcaattaga aatttcctta aaaaaataga tttagatata aaactaagtg
    16021 atttaggtat tgaaaaatca gatatagatt ggttaactga taattgtatg aaaatatcag
    16081 ttgcaaatat taagagacat ccaaagtatt ttaataagga acaaataaag gaaatgtacc
    16141 ataagtcttt ataatttgac tttacatatt tagttttata taaaaattat aaattaataa
    16201 taaaaaatta taagtaatta atagtaaaaa aattgtgtat agaagttata tatataataa
    16261 acttgtctta agcttttatt ttactttcta ataaataaag gagtttaaaa aagggaagat
    16321 taattaagta atattttata aaattcttcg atttaaaaac tttaagtaga ggtgaaataa
    16381 atgtttaatc ccaaaagaat aatattcgaa aaaggaactt tagacactga tgtgggcaaa
    16441 aaaatataca ataaatttag agataataaa gatgtagaga taattaatat atcatcaaat
    16501 aggattaaag aacacattcc aggtgaaaat ctatatgaac aatatagaag tgggaagcag
    16561 actatggtag ttgggattag aaaaagtctg aagtttcaaa catgtaaacc ttctgctaat
    16621 tatcaactac cacttgtcag tggatgtatg ggaagatgtg agtattgtta tttgaacaca
    16681 caattaggtg ataaaccatt tataagggtg tttgtaaatg tagatgaaat acttgaacag
    16741 acaaaaaaat atattgaaga tagaaagcca caggtaacca tatttgaagg ggcagctaca
    16801 tcagacccag ttccagtaga gccatatact aattctttga agaaaacaat agagtttttt
    16861 ggaagagagg aaaaaggaag atttagattt gttactaaat acagtgatgt ggatagctta
    16921 ttggatgctg aacataataa tcatacagaa atacgattta gtatcaatac tcctagaatt
    16981 ataaatgaac atgagcatta tacagcctct gcaaataaaa gaatagagtc agctgtaaaa
    17041 cttgctaggg ctggttatca gattggtttc ataatagcac ctgtgttttt atatgaaggt
    17101 tggaaagaag agtataaaaa tctgattata agtataaaag aagctttacc aaaggatttt
    17161 gataaaaaaa ttatatttga agttatatct catagatata caactaaagc aaaaaataga
    17221 atactagaga tttatccaga tacactattg ccaatggaaa atgaggatag aaagtttaaa
    17281 tacggtcaat ttggatatgg taagtacgtg tatgataaag aacaattaga tgatatgaaa
    17341 gagttctttc ttaaggaaat aaatggagta tttaaaaata gtttaataaa atatataata
    17401 tagcaaatct tacctaaaga tttgctatat tttattttta tatattttat gtttataata
    17461 aagcaatgag atttagcact atcatataag aaaattaaaa agggcactat ctatcaagaa
    17521 cattttttct agttttgata ctataaaggg taagagtaac aaaaatatca ggaggagtta
    17581 tagtatgcat gcatgggagt ctattcaaat tacattggat ttaatagaag caaatttatc
    17641 agaagaaata tcaattaatg aattagcaaa taaagcaaat ttatcaccat tttactatca
    17701 aagactattt aaaagattgg taaataagac tgtaatggag tatataaagc ttcgtcgtct
    17761 tgcaagagct tctgaatact tagcagaaca taagaatcgt atacttgatg tagctttaaa
    17821 ttttggattt gctagtcatg agacatttac acgttccttt aaaaaagcat atggattaac
    17881 tccagaaaaa tatagagcaa atccagtaaa actcaatcac tttattaagc cagaattaat
    17941 tttaaattat gctatggttg atgaaggtgt tccactgatt gcagatgata tagtgattga
    18001 agtcaatcgt aagacattga gtaatccaag gacatttgtt ggaatagaaa tagaagtacc
    18061 tatttgccag ttggtgggtg gtgaaacaac tggaattgca atagtagagg aattatggat
    18121 gaaacttgga agtcaaagac ataatatacc aaatcaaatt ccaggtggaa atgaatttgc
    18181 tgctctttat atgggaaatg caaaagaagg aaattgtatg tatatggcag gtgtagaggt
    18241 agagtcaggt acatcagtag aaggatataa tacttttgag ttgccagcta aagaatattt
    18301 agtatgtgga tttgaggcag aaaactttaa tgagttagtt aattcagctg tttttaaagc
    18361 agataaattt atggaaagat ggatgaagaa acataattta acaactactg attttgcaat
    18421 tgagatgtat taccccacta cacctgaagc agcatatctg gaacattgga ttgttccggt
    18481 tcctattgaa caatagaaag gatgatgaat atgaattggc aagatgcgtt ttttcaagat
    18541 actaaaccta catttgagca aattaatgaa tttattaata atccattatg ggacaagtta
    18601 aataaaactt tggtttctac ttataatgta gaacctaagc ttgaatacag taaatgttca
    18661 atgcaaagag gttggaatgt aaaatataaa aaaagaggta aatcactatg tactttatat
    18721 ccacaagaag gttattttaa agctttggtt attgtaagtg aaagcaatag agtagaggtt
    18781 gatttattta ttaatacttg ttgtgattat atcaaaaaaa tatataatga ggtaaacttt
    18841 tttaatggaa gtaaatggct aatgattcaa gtagatagtt tattggtatt aaacgatatg
    18901 cttgaactaa ttaaatttag agcttaacat atagaacagg atgaatatat aagtcatcct
    18961 gtttttatat tcaatcagat aaattaatat atagtctata atatgaataa tttagtaaat
    19021 ttactttatc tttctacatt ctttgcaact aagtccacat gtgttttttt cttgcatatt
    19081 agtagcaccc caattagcaa attgttgtaa aataggcaca atacttttac ctttacttgt
    19141 taatgtatac tcaacttttg gaggaatttc attatattgt ttacgatgaa caataccagt
    19201 agactctaaa tcttttaatg attgagctag catagtattt gtaatgccat ccaattttct
    19261 ttttatgcta ctatatctaa ttggattttc aactgctaac atacatataa taggtaattt
    19321 ccacttacca ccgacaatgt ctaatgcata acggattgga caggttaatt cttcaacgtt
    19381 tttagttata tttttttcag tatttatact cattttaatt actccttagt tatagtagta
    19441 tcttttttat actttatcag aaaaaactgc atacttgttt tttctgtatt ttatattaaa
    19501 attatatcaa aggataatcc ttaatacaag tatttaaaag gggtgtaaga attatgaaag
    19561 cattagaatt tttgaacaag gcaggagttt attatttagc aacagttgat gagaacaatc
    19621 aagcacatgt gcgtccttta ggatttgtta tggaatacaa tggtaaactt acattttgca
    19681 catctaatca aaaggatatg tttaagcaac ttatttttaa tccaaaggtt gaactttgtt
    19741 gtatagacca aaaccttaat actctacgta ttttaggtga agcagtattt gttacatcaa
    19801 aagaaacaca agctaaggca cttgaaataa tgccaagttt aggtcagatg tactctgtgg
    19861 gagatggtaa gtttgaaata ttctctattg ataaggcaaa agttagttgt tgttcaatga
    19921 gtggtaaaaa gattgatgta gagctttaat tataattgaa tcgacatcca tagtgaaaat
    19981 taagaaaacg ttattactga aatattgata gatttaattt aagtgcaata aaaagatttt
    20041 taaataatga atatgtaaaa atttttagtt attagatgtg tatttcaata atgatatact
//
LOCUS       DACTGW010000051        20091 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.051, whole genome shotgun sequence.
ACCESSION   DACTGW010000051 DACTGW010000000
VERSION     DACTGW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.051"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            144..1214
                     /locus_tag="I9573_001480"
     CDS             144..1214
                     /locus_tag="I9573_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796106.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGKLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1198..2067
                     /locus_tag="I9573_001481"
     CDS             1198..2067
                     /locus_tag="I9573_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAT4796107.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2111..2971
                     /locus_tag="I9573_001482"
     CDS             2111..2971
                     /locus_tag="I9573_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAT4796108.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSMEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLILQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYIPLIIPFMTS
                     SFGIFLLRQNFMQISDETIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3002..4306
                     /locus_tag="I9573_001483"
     CDS             3002..4306
                     /locus_tag="I9573_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT4796109.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INRDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4369..5631
                     /locus_tag="I9573_001484"
     CDS             4369..5631
                     /locus_tag="I9573_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HAT4796110.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKVKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFDLKLDGVEKEAFDFIM"
     gene            5818..7788
                     /gene="uvrB"
                     /locus_tag="I9573_001485"
     CDS             5818..7788
                     /gene="uvrB"
                     /locus_tag="I9573_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAT4796111.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            7807..10632
                     /gene="uvrA"
                     /locus_tag="I9573_001486"
     CDS             7807..10632
                     /gene="uvrA"
                     /locus_tag="I9573_001486"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAT4796112.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            10891..12708
                     /gene="uvrC"
                     /locus_tag="I9573_001487"
     CDS             10891..12708
                     /gene="uvrC"
                     /locus_tag="I9573_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAT4796113.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVEAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            12708..13637
                     /locus_tag="I9573_001488"
     CDS             12708..13637
                     /locus_tag="I9573_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAT4796114.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            13851..15647
                     /locus_tag="I9573_001489"
     CDS             13851..15647
                     /locus_tag="I9573_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HAT4796115.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQILSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLSPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(15787..17568)
                     /locus_tag="I9573_001490"
     CDS             complement(15787..17568)
                     /locus_tag="I9573_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HAT4796116.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRICMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17591..19477)
                     /locus_tag="I9573_001491"
     CDS             complement(17591..19477)
                     /locus_tag="I9573_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAT4796117.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19494..19991)
                     /locus_tag="I9573_001492"
     CDS             complement(19494..19991)
                     /locus_tag="I9573_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HAT4796118.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLTTKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
BASE COUNT         7744 a         2668 c         3473 g         6206 t
ORIGIN      
        1 acaaaaaatg tttataattt aacctttatt ttaataagtt tactatatat tcactttgtc
       61 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      121 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      181 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      241 cagtattggt aggtccaagt ggatgtggta aatctactac attaagaatg attgcaggtc
      301 ttgaagatat aacttcaggc aagttaaaaa ttgaagaaaa tgacgttaca aaaacggatg
      361 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      421 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      481 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      541 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      601 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      661 tgagaacaga attaatacaa ttacatcaaa aactaaaatc tacatttgtt tatgtaactc
      721 atgaccaggt tgaggcaatg tcaatggcag ataagatagt tataatgaac aaaggaaaga
      781 taatgcaaat aggttctcca aagaagatat atcaaaatcc taagaattta tttgtagctc
      841 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
      901 tcagaccaga aaaagcaaca ctagagagta taaacataga tggaaaaaat gatgatgtat
      961 ttagcctatt tggaaaagtt gtgactaaag aagtgttggg aaatgaaact atatattgtt
     1021 tatctgttct taatagagaa ataagagtca aaacggaaaa tgatatattt gatattggaa
     1081 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1141 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aattaaagga gatatttatg
     1201 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1261 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1321 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1381 tttatacaaa ctataacgaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1441 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1501 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1561 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1621 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1681 tggaagactc ttggatacaa tatcttaata gtattatcag gtcttaaatc cataccaaac
     1741 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     1801 acattgccgc tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     1861 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     1921 atgttatctt attatatata tgagtctgga tttatatact atgacatagg gcaagcgagt
     1981 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2041 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataagat ttatccaaaa
     2101 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2161 agcatcaaaa tcaatggaaa tattaattct attactttta tctgcaatat ttatattccc
     2221 ttttatatgg atgctatcga catcctttaa aaatccacaa gaaatgatgc aattaccacc
     2281 tactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2341 attttttaga tatttactaa acagtgtatt tgtaacagga agtgttttaa ttttacaatt
     2401 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2461 gttatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2521 ctatatgatg atgagtaagt ttggatttat aaatacttat attccactta taattccatt
     2581 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2641 aactattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     2701 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     2761 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     2821 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     2881 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     2941 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3001 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat taggttcttt
     3061 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3121 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3181 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3241 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3301 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3361 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3421 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3481 cataaataga gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3541 agagcttaag gattacgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3601 aacaccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3661 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta aattctggca
     3721 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     3781 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     3841 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcctacc
     3901 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     3961 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4021 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4081 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4141 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4201 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4261 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4321 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4381 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4441 aactttaaat ctatactaga aaattgctcc atagtaaaaa atataaattg tgtttatccc
     4501 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4561 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtattca
     4621 aaggacataa aagtaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4681 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     4741 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagctc cagtaaggtt
     4801 ataatggacg gtatttacaa tagacattgt cattatattg attggaaatt agaacccaac
     4861 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     4921 atgctaatac atttagctac attggaccat acacgacata attatggctt atttaatgac
     4981 gaagtagata aagcattaaa aatgaatgat aaatggctag gagatattat tcaggctaca
     5041 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5101 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5161 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5221 ataatagtaa ataacttaga taggttaaca gaattaagag aactgcttga agaatttaaa
     5281 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5341 ggtgattttg aaatggtgat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5401 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5461 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5521 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5581 gatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5641 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     5701 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     5761 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     5821 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     5881 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     5941 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6001 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6061 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6121 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6181 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6241 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6301 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6361 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6421 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6481 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6541 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6601 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6661 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     6721 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     6781 actttaatgg atttcttccc agatgattat ttgataattg tagatgaggc acatgtcact
     6841 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     6901 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     6961 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag gaccttatga aattcaacac
     7021 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7081 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7141 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7201 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7261 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7321 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7381 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7441 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7501 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatata
     7561 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7621 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7681 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     7741 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     7801 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     7861 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     7921 tcttctttgg catttgatac aatatatgca gaagggcaga gaagatatgt agaaagtcta
     7981 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8041 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8101 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8161 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8221 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8281 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aagaaggatt tgtaagagtt
     8341 agagttaatg gtgagaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8401 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8461 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8521 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8581 gaagaattat ctccaaggat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8641 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     8701 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     8761 tacagtaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     8821 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     8881 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     8941 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9001 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9061 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9121 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9181 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9241 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9301 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9361 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9421 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9481 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9541 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9601 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9661 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgc
     9721 ataacaggtg tttcaggttc tggaaaaagt acattaataa acgatatact ttacaaaggt
     9781 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
     9841 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
     9901 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
     9961 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gttttaatgt aaaaggtggt
    10021 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10081 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10141 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10201 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10261 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc gcaacgtata
    10321 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10381 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10441 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10501 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10561 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10621 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10681 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    10741 ttactcttca tggttaaaat tgttttcaaa tcaattatag aagggaatat ggactttaac
    10801 ttgtctaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    10861 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    10921 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    10981 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11041 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11101 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11161 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac gaatgaagat
    11221 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttggaccc
    11281 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11341 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11401 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11461 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11521 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11581 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11641 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    11701 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggtgta
    11761 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    11821 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    11881 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    11941 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12001 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaaacaaata
    12061 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12121 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12181 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12241 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12301 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12361 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12421 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12481 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12541 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12601 gtagaggcta taaaaaaagc tactatggaa gaacttttag aagtagatgg aatgaataaa
    12661 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    12721 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    12781 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    12841 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    12901 tttttggatt aatggatagt catattcgag aagaagtact agatagattt ttctcatatg
    12961 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13021 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13081 gactgtcaaa ctatttggat aataagttag caccacacac aacaattcac ggggtgctag
    13141 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13201 cagcattaga actgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13261 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13321 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13381 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13441 atagactagg actggataaa gaatacgaag aaatacttgg tgttaatgta gagaaattag
    13501 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13561 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13621 taatcgatac taagtagtac tgaataatag tgaactacac atcatttaat taaatattga
    13681 ttaggattat gtcctacacc ttataaatta aaatcaagtt gtatatatat aatgtttatt
    13741 aataagatat attatataag gataatttaa agtacagtgt tagattataa taatactaga
    13801 ttaattttat atttgagttt cattatataa caagaaagtt ggaatttttt atgggaatag
    13861 aatattttag taaactttta gaaagtagta ctgaaaaaga attgctctta agtaaaaagt
    13921 caaataacat aagtacagca agattaatat cttttttgat agtgatagca gggtttgcta
    13981 ttggatttta caataaaaat atagtaggta tttttgttgg agttttatca ataataatat
    14041 ttatagcact cttagttatt catagtaaag taaaagaaga agaaacatat tttaaaagta
    14101 agagcgaagt attaaataag tatattaaga gatttggaga tggttggaag gaatttaaaa
    14161 tagatggtaa agaatactta aaagatgaaa attcgcaagc aaaagattta gacttatttg
    14221 gaagagcatc attgtatcaa tatatctgtg tagctaatac atcttatggg aaaaattttt
    14281 tagccaaata tttgtggaat gaaaatccag atgagaatgt aatcttagaa agacagaggg
    14341 ctataaaaga acttttatca aaagatgatt tttctattca tattcaaata ctcagtaata
    14401 taattggtaa agagcaaaaa aataattcag atagtagtat agaatcgttt atagagtatg
    14461 gagaaaataa gaatgtgtat ataccaaaat ggatgcatat ttttacttgg ggtctaccaa
    14521 ctgcaacaat cttatctttt atattttgta tgttaggatt tttacctgtt ttacctgttt
    14581 ttttactttt tataatccag ttagggttta gtggttttgg atatccaaga cttatgcaaa
    14641 ctctttctcc attattttca tttagtagaa gtatacaagt atatgaaaaa atgtttgagg
    14701 ttttagaaaa agagacgttt gagagtgcat atttgaaaga attacaagat aaactttcta
    14761 agggaagtgg agtttcaaga ggtgttaaac aattaaattc aataggaaat gctgtaaacc
    14821 taaggtataa tcagatacta tatataattg cctgtggtgt tttgatgtgg aactatcatt
    14881 gtgcagaagc tttggagcga tggaaaggta tatatggaaa tcaaattaga gattggtttg
    14941 aatctattgg agaatttgaa gctctaataa gtttaacagt tatttctcat gtaaaggaaa
    15001 atacatgttt tccagtaata aaatatgaag atactccaag attgaaagtt gaagaggtat
    15061 accacccatt aatagcagaa aaaagtgtca tagcaaattc gatacaatta aattctcaga
    15121 cttgtattat cacaggttca aatatgtctg gaaagacaac atttttaaga agtatcggtg
    15181 taaacttagt gctggcatat gcaggtgctc cagtatgcgc taagagtttt gatgcgacct
    15241 gtatggctat atttacttct atgagaatac aagatgatgt aagtcaagga atatcaactt
    15301 tctatgctga gattcttaga ataaaatcaa tgattcaata tagtttaaaa gaactgccaa
    15361 tgttggttct agtagatgaa atttttaagg gaactaactc tgctgataga attattggag
    15421 caagtgaggc agtaaagaaa ttatcaaaac cctgggttat aagtatggtg acaacacatg
    15481 actttgagct atgtgacctt tcaggtagtg gagatgttga agttgtaaac tatcatttct
    15541 cagagtacta tgtagacgat aaaattcatt ttgactataa gataaaagat ggaagatgta
    15601 aaactacaaa tgcaaaacaa cttatgagaa tggcaggtat tctttaatta atagataggt
    15661 taattaaaga ataagtctat ttagaaatat aatttaattt gtgaaattaa taaggattgt
    15721 cttaaattac gtaaaagtat tttaagacaa tcctttttta agaataaatt ttatactttg
    15781 ttatgactac ttagcttctt cacaagcaca ttcttgtgga cactctccat agacacaact
    15841 cttatcaaag taatgagtat gaaggtattt atgggctaca tcactatttg gttctccaaa
    15901 gaagtcttta taaatagctt gtaaatcagg attctcatga gatttacgaa tagccttatt
    15961 tctatctatt tcatataacg catttgcacg tctatcaact atttcagtat taccatggtg
    16021 ataaggttga ccagcacctc caacacatcc accaggacat gccataactt ctatgatatg
    16081 gtatttacat tctccacttc tgaccttatc cataatcttt ctagcatttt taagtgtatt
    16141 tacaatacac acattaactg tagttccatc aacatctata cttgcttccc taattccttt
    16201 aagacctcta acattagtga agtttacatt atctaaagtc ttattagtta tcttctcata
    16261 tgaagtacgt agggcagctt ctaaaactcc accacttgct ccaaatatag atgcagcacc
    16321 tgtagatttt cctagaggat tatcaaaatc ttggtcttga agattaggta aatcgatagc
    16381 agcttctttt atcattttag ctaactctct agtagttata gaaaggtcaa catctagtat
    16441 tccagattca cttaattctt ctcttgaagc ttcatatttt ttagccacac aaggcattac
    16501 agatacaaca taaagttctt caggattgat tcctaatatt ttaggtgcat agtagttttt
    16561 agctatagaa ccaaacatac cttgaggaga cttacagcta gatgctaagt ttaacttgtc
    16621 tggataattg tgttctaaga agtttaccca tgcaggacaa caacttgtta atataggaag
    16681 attttcacct ttttggattc tttctataaa ttcagtagct tcttccatta tagtaaaatc
    16741 tgcaccaaag tttgtatcaa atacatgttc aaatccaagt gcttttaaag cagctaccat
    16801 ttttcctgta gagattgacc ctggttctaa tccaaactct tcaccaatag caactcttac
    16861 agcaggagca acttgcacta ctatagtctt ttctttttta tttaaaacat cccaaagttt
    16921 aggtacatta tctacttctg ttaatgctcc agttggacag acagatatac attgaccaca
    16981 gaatgtacac tctgtttcaa ccatatcaga gttaaagaat gtactaacta aagtattaaa
    17041 tccacgatta actccagata atgctcctac tgtttgtatg tcattacata cagtctcaca
    17101 tcttctacat aatatacact tagaatggtc tttaactaaa gatttagtag aagtatcttt
    17161 acctgcaaaa gatttggaac cttgatatct tatttttctt actcctaaat cagcagctat
    17221 agtctgtaat tcacaatctc catttttttc acagacaaaa caatcttgtg gatggtctga
    17281 taacaataat tcaacaatag ttctacgagc atttaatgct ttagcactat tagtctgaac
    17341 tttcatacct tcctttataa ctgttccaca agctggaact aagcctctct ctgtctcaac
    17401 catacaaata cgacaagaag cacaagtgtc aagcttatct atttcgttca tatgtaaatg
    17461 acaaaggttg tgtattttta tatttattaa tttcgcagca tctaagatag aagttccact
    17521 aggtgctgaa acgtgtttgc cattaatagt taaattaact aaactcattg ttattcctcc
    17581 tccaaataag ttatttcttg attatagcgt taaatgtaca attatctata caagcaccac
    17641 attttagaca cttagaagta tctattgtat gttgttcctt aacagaacct gttatacaac
    17701 cagcaggaca cactctagaa cattttgtac aacccttaca gtcatctgtt ataaagtaag
    17761 aaagtaagtt ctgacattta ccagcaggac atttcttatc tacaacatga gctatatact
    17821 catctttgaa atattgaaga gtacttaaaa cagggtttgg agcagcttta ccaagaccac
    17881 ataatgaagc agtttgtata gtttcagcaa ggttttctag gtcatctaaa tcttgtagag
    17941 taccattacc ttctgtaatt ttagttaata tttctaataa tcttgtagta cctatacgac
    18001 aaggtgtaca tttaccacaa gattcttcaa cagtaaactc aaggaagaac ttggctatat
    18061 ctaccataca gtcagattca tcaagaacaa gcataccacc agagcccatc attgaaccga
    18121 tagaacttaa tgaaccaaag tctataggag tgtctaaatg ttcagttggt atacatccac
    18181 cagatggtcc acctgtttga acagccttaa atttcttacc ttcagggatt cccccaccaa
    18241 tttcaaatac aatatctctt aaagtagtac ccattggaac ttcaacaagt cctacatttt
    18301 ctacttttcc accaagagca aagactttag taccactaga atcttcagta cctaagttgg
    18361 caaaccaatc tccaccctta agtataattg caggtatgtt agcaaaagtc tcaacgttat
    18421 ttaagcaagt aggagatttc catagaccac tcttagaaga actataagtt ttcattctag
    18481 gttcaccacg tctaccttca attgaatgca ttagcgctgt accttcacca caaacgaaag
    18541 cacctgcacc atattttaat tctaatttaa agttaaatcc agtgcctaag atattttcgc
    18601 caagtaaacc gtatttttca gcttgagcta tggctacttt taatctttct atagattttg
    18661 gatattctgc tcttatgtat atgtatcctg tatcagaacc tatagcgtat ccacatattg
    18721 ccatagcttc tagtacacta tgagggtcac cttcaagtac agatcgatcc ataaatgcac
    18781 ctgggtcacc ttcatcagca ttacatacaa caaacttttt atctgttgga ccagctggat
    18841 tttttgaagc agcttcccat ttagagccag tagggaaacc tgcaccacct ctaccacgaa
    18901 ggccagaagt ttttacctct gcaataactt cttgaggagt aagagaagtt aaacatttgc
    18961 ctagagctaa ataaccatca ttggcaatat attcttctat actatctggg tctataagac
    19021 cacaattttt taaagctata cgtaactgtt ttttgtagaa agacatttct tcttgttctt
    19081 caactttttt acttaaagat tcttcctcat ataataattc ctcgacaact gtatttctaa
    19141 ttaagtggct ttgaactatc ttttcagcat cagatggctc aacttttacg tagaaaacgt
    19201 tatcaggata aacttttaca ataggacctt gagcacagaa accaaaacaa cctgtcaaac
    19261 gaacactaac tttatcttgg attcctgatt tctttatttc attttctaat tcactaacta
    19321 tttctagact gtttgaagag gtacatccag tatcacaaca aacaagtaat tctcttctta
    19381 atacattttc cttagaataa ttttttctta agctaagatt aggtttcagc tcatcagcta
    19441 aaacttttaa ttcatcaaat gaatttactt tacgcattat ttattcaccc ctcttaacaa
    19501 ttcaattcta attctctata agtatctaat atttcgctta cttggtcagg tttcacttta
    19561 ccataaactt taccatttac agtaacaact ggagctaaac cacatgcccc caaacatctt
    19621 aaaccctcta aagaaaattt aaaatcacta gtagtttctc caacttttat gccaagctgt
    19681 ttttcaaaag cacttaagat tttgtcagca ccttttacaa aacatacagt tcctagacag
    19741 acactgattt tatacttacc tacaggttct gtagtaaaat aagaatagaa tgtaacaacc
    19801 ccatatacct ttgcaggagc tactccaagt tttctagcaa catgaagttg aacttctttt
    19861 ggaagatatc caaatatacc ttgggcttca tgtagaactt gtattaacgc accttccttt
    19921 gttgtaagag agtcaataaa tatatctagt tcgtcaaata attgcttgtt gtgtgaaata
    19981 aaatcgcaca taacaaatcc cccttttctt tttaaagtta ttatttttca taaaataatt
    20041 aaccatgagt tgaccccaac attatgtggg tgaacaaaac cagaacaata a
//
LOCUS       DACTGW010000052        19690 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.052, whole genome shotgun sequence.
ACCESSION   DACTGW010000052 DACTGW010000000
VERSION     DACTGW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.052"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            81..1148
                     /locus_tag="I9573_001493"
     CDS             81..1148
                     /locus_tag="I9573_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HAT4796119.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGDVLIEMEHPQQIIPEVE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            1245..1553
                     /locus_tag="I9573_001494"
     CDS             1245..1553
                     /locus_tag="I9573_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HAT4796120.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            1546..2976
                     /locus_tag="I9573_001495"
     CDS             1546..2976
                     /locus_tag="I9573_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HAT4796121.1"
                     /translation="MNNSTSIKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGAKIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            3054..3428
                     /locus_tag="I9573_001496"
     CDS             3054..3428
                     /locus_tag="I9573_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase"
                     /protein_id="HAT4796122.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWNLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            3422..5626
                     /locus_tag="I9573_001497"
     CDS             3422..5626
                     /locus_tag="I9573_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HAT4796123.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            5626..7035
                     /locus_tag="I9573_001498"
     CDS             5626..7035
                     /locus_tag="I9573_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HAT4796124.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELYIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            7020..8084
                     /locus_tag="I9573_001499"
     CDS             7020..8084
                     /locus_tag="I9573_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HAT4796125.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERSIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKKLQDINLPKILIRIPMLSELEEVINYCDISFNSELETI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            8114..9535
                     /gene="nhaC"
                     /locus_tag="I9573_001500"
     CDS             8114..9535
                     /gene="nhaC"
                     /locus_tag="I9573_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAT4796126.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            9964..10428
                     /locus_tag="I9573_001501"
     CDS             9964..10428
                     /locus_tag="I9573_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT4796127.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            10726..11088
                     /locus_tag="I9573_001502"
     CDS             10726..11088
                     /locus_tag="I9573_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAT4796128.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            11683..12543
                     /locus_tag="I9573_001503"
     CDS             11683..12543
                     /locus_tag="I9573_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4796129.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIIANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            12801..14915
                     /locus_tag="I9573_001504"
     CDS             12801..14915
                     /locus_tag="I9573_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4796130.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEHDVELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYVGTLTQLISM"
     gene            complement(15177..17420)
                     /locus_tag="I9573_001505"
     CDS             complement(15177..17420)
                     /locus_tag="I9573_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAT4796131.1"
                     /translation="MSNIIIKGLTQNNLKNISFKIPKNKIVVFTGVSGSGKSSIVFDT
                     IAAESQRQMNETYSSFIRNRLPKYRKPQVELIDNLTASVIVDQTPLGGNARSTVGTIS
                     DLYSSLRLLFSRIGEPYIGTTSYFSFNDPNGMCKNCSGIGRVKVINIESIINPENSWN
                     TGCINDSLFKPNSWYWKQYAESGLFDLDKPIKDYSFEEYNLLLYGSKNKDGKQENPKI
                     EGIFNKYNQTYLNRDISNMSKHTKEKSERLITDKECPVCHGKRLNKETLNCNILGYSI
                     ADMCNMELTDLYDLLLQIKDKRTETVIKTLTDGLKRMIDIGLPYLHLNRESSSLSGGE
                     AQRLKLVRYMGSSLTGMTYIFDEPSAGMHPRDVHRMNQLLKKLRDRGNTVLVVEHDKD
                     VISIADEVIDIGPSAGTQGGHVVFQGSYDKLLLANTLTSNAMNLSLPIKTDSRHPKGT
                     LPIRDACINNLKHIDVDLPVGIMTVVTGVAGSGKSTLISRVFAQKYEKDVIKIDQSPV
                     TATNRSMPATFLGFFDEIRKVFAKENNVEEGLFSFNSKGACPVCDGKGVLVTELVFMD
                     PIINTCEACKGTRYSEEVLSKLYKGKCIVDVLSMTVDESLEFFTEKKLCKILQTMKDV
                     GLPYMTLGQPLSTLSGGERQRIKLAKNLNKKGTIYILDEPTTGLHGSDIEKLMLIFEH
                     IVDKGNTVIIIEHNLDVLKQADWVIDIGPDGGKNGGQVVFEGTPLEMFERGCTITSDF
                     LRKSINI"
     gene            17752..18468
                     /locus_tag="I9573_001506"
     CDS             17752..18468
                     /locus_tag="I9573_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796132.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            18496..19434
                     /gene="blaCDD"
                     /locus_tag="I9573_001507"
     CDS             18496..19434
                     /gene="blaCDD"
                     /locus_tag="I9573_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HAT4796133.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT         7596 a         2271 c         3529 g         6294 t
ORIGIN      
        1 tttactttaa ttttaaagtt ggcacgattt atgctttaac taattattga gaatatataa
       61 atttttagga gggtgtatat atgagaaaag taagagtagg aatatgggga tttggagcta
      121 tgggaatagg tatggctaat atgattctaa agaaagaagg tatagaaata gtatctgttt
      181 gtagtagaag tacttcaggg aaaagtatgt atgatgtttt aggaattgaa agaggagaaa
      241 gaccagaagt tattatcaat aaaaattatg aagaagtttt tagagaaaaa agtgtagatg
      301 ttgttttgtt agcaactgat tcatttacta aaaaggcttt tgataaaata attttcttat
      361 taaatagaaa aataaatgtt atatcaacag cagaacaaat ggcatatcca caagctgatg
      421 atgcagattt agctaaaaaa atggatgaag ttgcaaagga aaatggagta agtatacttg
      481 gaacaggaat aaatccagga tttgtattag atttactagt attggcactt tcaggtactt
      541 gtgaagaagt tactagtata aaagctaaga gagtaaatga tttatctcca tttggaaaat
      601 ctgttatgga ggaacaaggt gttggagtaa caagggaaga atttataaaa ggtgtagaag
      661 ataagactat agcaggtcat gtaggttttg tagaatcaat aaatatgata gctgatggat
      721 taggttggaa attagataag atagaacaaa caaaagagcc aataatgact acagtagata
      781 gaaaatctaa atacggagag gcattggctg gaaatgttgc tggttgtaga caatgtggat
      841 atggatatgt aaatggagac gttttaattg aaatggagca tccacaacag ataataccag
      901 aagtagaagg tattaaaaca ggagactatg tatcaataaa aggtatacca aacattgatt
      961 tacaaataaa tcctgaaata cctggaggag taggtactta tgcaatgata gtaaactcta
     1021 ttccattaat aataaatgct agaccaggtc ttaaaactat gttggatata ccagtgccta
     1081 gagcaataat gggagacata agaaatcaga tagaagttga attggaagaa gaatcaaagg
     1141 ctaattagta agataacagt tagctaattg gtaaaataat gtttaattta aaataacagt
     1201 aaattaatta gtaaggaata gtcaattaat aaggggatga aaaaatggat gctaaaatta
     1261 atgattgggt tattatacac aatatagtat tgactccaga agaaagagct ccacaagttc
     1321 cagaggatac taagaaagta tccttagaaa tgtgggttaa aggttttata caaagtgatg
     1381 cttctatagg cgacttagta gaagttaaaa caattacagg tagacttgta aaaggtaatt
     1441 tacttaaagt aaatccatat tatactcatg attatggtaa atgtatacca gagttactcc
     1501 aaataggaat ccaagctaaa gaaatattat ttggaggtgt atataatgaa taattcaaca
     1561 tcaataaaag atatgagtta tcaagctgtt atgggaagaa ataacgaaat aatgaaaaat
     1621 gcaatcggat tggattattc ttcttttgaa caagagggaa tagggtttga ttatgagaaa
     1681 atgatgagtg aaacaggata tacattacaa gatattgaag caattcaatc acagtatgct
     1741 gttggaaata ctcctctaat agagcttaaa aatttgacta aattagctag aaaatgtgct
     1801 aaagaaggta aaggagctaa aatatttgta aaagatgaag caatgaatgc atctggtagt
     1861 tttaaagcaa gaagagcagc tactgcagta tatcatgcca aacaaatggg atataaaggt
     1921 gtaatagcag ctaccagtgg taactatggt gcagctgtag catcacaagc ggcaatgcag
     1981 ggattaaaat gtataatagt tcaagaatgt tatgattcta atggtgtagg tcaacctgaa
     2041 attatagaaa aagcacgaaa atgtgaggca tatggagcag aagttgttca gttaactgtt
     2101 ggaccagagt tattttatac gttcttagtt ttattagaag aaacaggcta ttttaacgca
     2161 tcattatatt caccatttgg aattgcaggt gtagaaactt taggttatga gttagctatc
     2221 caatttagag aaaagtataa aaaagaccct gatatagttg tttgtacaaa tgcaggtggt
     2281 ggtaatctta ctggaactgc aagaggattg ataaaagctg gttccattaa tactaaagta
     2341 gtaggagcaa gtgtagattt aaaaggatta cacatggcta gtgataatca atttaataaa
     2401 aaatcattta caacaggaca tacaggtttt gggataccat actgtacatg gcctgatagg
     2461 tcagatgtac ctaggtctgc agctagacca cttagatata tggatagata tgtattagta
     2521 aaacaaggag aagtttttta tacaactgaa ttattggcac agctagaagg tatagaaaga
     2581 ggtccagcag gaaatacatc tttagcagca gcattctctt tagctcaaga gttagatgag
     2641 gataaagtta tagtggtaca agaaacagaa tatacaggcg caggtaaaca tatatgtcca
     2701 cagcttactt ttgcaagaga aaatggaata gatataaaat ttggaaatcc aagagaagaa
     2761 attgcaggag taaacttaat attaccagaa agaccagaat tattgaaatg tgttgatatt
     2821 gatatgaata aaataagaaa gtcatttata aaaaattgca tatctaataa tcacattgat
     2881 aatattgata agctaagtaa tcgagatata gaatttttaa tggaagaagt aaaaagttca
     2941 agagattttg ttgttgatgt attaaataat ttatagtaat tattgttttt ttatttaata
     3001 taaaataaaa attcggaaaa ttctattaaa tcaatgcgaa tggaggaatg ttcatgaaaa
     3061 aaagagagga tgattttgaa gttagaagaa aacatcttca agaactgagt gatgatgagt
     3121 taaaagaaag gttttggaat ttagctaccc agatagtaga accaatgata gaacttggta
     3181 aaaagaatac aacaccatca atagaaaggt ctgtcttgtt gagaatggga ttttcttctc
     3241 ttgaagtaaa acctatttta gaaggggtta tggaaagagg tcttataggt aaaggagcag
     3301 ggcatgtagt ttataaattg gctaaatcta aaaatataac ggttagagaa gcaggtttgt
     3361 tattagttaa gggtgaatat tgggatgaag ttacatgttt atttagagaa ggggtagaat
     3421 catgttaaag gaaaataaaa aattggatat agactatata ttaaaagatt tggataaata
     3481 taaaccaaaa cgaagaggtt gggtttggag agaacacttg gaaaatcttg aaatgggacc
     3541 ttttaaatat aaagactgta caaaaccttt aaagaagagt gtagggctac catcatctaa
     3601 gtatttcaat aatatagacc ctcaaccaag tccagttatt actactgaaa tagcttctgg
     3661 tagatttgag gatgatataa gaagaatgag aatggcagct catcatggag ctgaccatct
     3721 aatggttatc agaactgctg gacaatcaca ttttgatggg cttatagaag gaactccaca
     3781 aggtataggt ggagttccaa taacaagaaa acaagtaaga gctcaaagga aggctcttga
     3841 ttttatagaa gaagaagttg gtagacctat taattaccat tcatatgtaa gtggtgttgc
     3901 tggtcctgaa gtagcagtaa tgtttgctga ggaaggagtc aatggagcac atcaagaccc
     3961 acaatataat gttctgtata ggaatataaa catggtaagg tcttttgtag atgcttgtga
     4021 ggcaaagaaa ataatggctt ttgccaatat agctcaaatt gatggagcac ataatgctaa
     4081 tgctactgct agagaagcat ggaaggtaat gcctgaactt atggttcaac acgctttaaa
     4141 ctctatattc tcagaaaaaa taggtataga taaaagtaat atatgcttat caacagttcc
     4201 accaacagca cctccagcac catgtttaag aatagactta ccttacgctg ttgcacttag
     4261 agaattattt agtgattata agatgagagc tcaaatgaat actaaatata tggaatcatc
     4321 aactagagaa gcaacagtta ctcatgtatt aaacttatta acatcagtat taactagagc
     4381 tgacatacaa tcaactataa cacctgatga aggtagaaat gtaccttggc atatatacaa
     4441 tatagaagca tgtgatacag caaagcaagc tttagttggt atggatgggc ttatggatat
     4501 gatagaactt aaagatgtcg gtgaattaag agacaaagca agagaactta aagaaagagc
     4561 tgtattatat atggaagaaa tatctgaagt tggtggatat tttgaatctg tggaacaagg
     4621 attttttgtt gattctggaa attatccaga aagaaatggt gatggaatat ctagaaaaat
     4681 aaaaggtgga gttggtgaag gtactgttta tgaaagagaa gaagattatt tagcacctgt
     4741 aactgctcac tttggataca ataatgtagc tcaatatgat gaaaatgcta tagataatcc
     4801 atcaatgtta atagatggat gtacatttga aaatccagac aaaataatat atattgatga
     4861 gcttgacgat tttgataatg tagaaaatag actaaaagaa tcatatgagt acagaaatgg
     4921 aactaaaatt aagcctgaaa tggaatggtg tgcagatgga attgtaatga taacaatgat
     4981 gcttcctact gataaaagaa cagcagagtt tgcagctttg gaatttgtca aaaaaatgaa
     5041 cttacaagaa attgaggtca taagtagaga agttatgcat gaatctgaag ggactagaat
     5101 agaagtgaaa ggaagagttc cgtttgatat agatttaaac aatttagtaa taccagaaga
     5161 acctaaggta ctaactgatg aagaaataag agcagatata gaagaaaaac ctatgaaaat
     5221 agtttcagct acggttggag aagatgaaca ttcagttggt ttaagagaaa taatagatat
     5281 aaaacatgga ggtattgaaa aatatggcat agaatgccac tatctaggga cttctgtacc
     5341 tgtagaaaaa ttggtagatg cagcgattga actgaatgca gatgcaatac ttgcatctac
     5401 aataataagt catgatgata ttcactataa aaacatgaaa aaattacacg attattgtgt
     5461 agaaaaaggt ataagagata aagtaatgat tgcgtgtgga ggaacacaag taacacctga
     5521 aatagctgtt gaacaaggca tcgatgctgg atttggaaga aacagcaaag gcatacatgt
     5581 agccactttc ttagttgaaa aaagaagaga aatgagtaat aaataatgga aaatagcaaa
     5641 gtttctttaa aaatagatgt gttggtagct gaaattggaa gtactactac agtggtaaac
     5701 gctttccacg atattaatac taacaatcct atatttttgg gtcaaggtca agctccaaca
     5761 acagtgtttg aaggtggcga tgtaagaaat ggcttatcag gtgctattaa agatttagct
     5821 aataaattat ctgtagatga tatagagtat aatgatatgt ttgcaacttc tagtgcagct
     5881 ggtggattaa aaatgactgt tcatggatta gtctatgata tgacagtaaa agcagctaaa
     5941 gaagcagctt taggggctgg agctgttatt cgtcaaataa catcaggtag aattaaaaga
     6001 actgatttaa ataaaataaa agaaataaac ccaaacataa ttttaatagc tggtggagtc
     6061 gattatggag aaagagatac agctatttat aatgcagaaa tgatagcttc tatgaatcta
     6121 ggcataccag ttatatatgc tggtaatgtt gaaaatcaag aggaaataag gttaatattt
     6181 gaagatacta attataagct ttatataact gaaaatgtat accctaaaat agatttattg
     6241 aatatagaac ctacaagaag aatcattcaa agtgtatttg aagaacacat aactacagca
     6301 ccaggaatga aatacattaa agaaatggtt aatgaaaata ttactccaac cccaggagct
     6361 gtaatggaag cttctaaact tttatataaa aatattggag atttattaac tttggatgta
     6421 ggtggagcaa ctactgatgt acattctgtt acagatggaa gtgactatat taataaaatt
     6481 ttggttaatc ctgaacctac tgcaaagaga agtgttgagg gggatttagg tgtatatgta
     6541 aatatgaaga atatagtaga agttataggt aaagaaaact tacaaagtga actatatata
     6601 gatattgatg cagtaataga gaattatcct ccaataccaa aatcaaaaga agaaatatta
     6661 tttgttgaga ggttgacaaa agaagctgtt gttaaagcta tgctaagaca ttcaggaaaa
     6721 ataagaaata tctatggaac aactggaaaa gtcaaaattg cagaagggaa agatttaaca
     6781 gaagtaagat atatagttgg tacaggggga gcacttacta ggctccctag tagaattgaa
     6841 atattagata atatgctcaa atacaataaa aataatgaat tactatttcc aaaggaaaaa
     6901 acaaagatat taatagataa tgactatatt atggcatcta tgggtgtttt atctaaaaaa
     6961 tatgaagaag catctcttaa gctcctgtta aaaagtttga attttgagga ggaaagatta
     7021 tgtatcctag gttagaaata gatatagaaa agttaaagca taatgcaaaa ttaatttcgc
     7081 agatgtgtca tgaaagaagt attaagatat cttttgttac taaatcattt tgtgcacaaa
     7141 aagaaatagt agaagaaata tgtagtgaag gaatagacca tatagcagat tcaagaatac
     7201 aaaacttaaa aaaattacaa gatataaatc tacctaagat attgattaga atcccaatgt
     7261 taagtgaatt agaagaagtt atcaattatt gcgatattag ttttaactca gaattagaaa
     7321 caataaagaa attaaatgaa ttatgtgaaa gtaaaaatat tattcataag atagttctta
     7381 tgtttgactt aggcgattta cgagaaggat acttttatga agaagacttt tttaataatg
     7441 taagagagat agtaagatta aaaaatattg aaataattgg tatagctaca aacttaacct
     7501 gttatggagc tattatacca tctagggaaa atataggcag actggtaagt atagcaaaaa
     7561 gaatggaaga aaaatttgga ataaatctag aaatagtatc tggaggtaat tcaagttcta
     7621 ttcatctgtt gataaataat aatatgccag aaggtattac taatttgaga ataggcgagt
     7681 ccattctact tggtagagaa acggcatacg gtgaaaatat aaatggaaca tatcaagatg
     7741 catttaaatt aatatgccaa gtagttgaat gtaaagaaaa accatcagtt cctattggag
     7801 aaataggagt tgatgccttt agaaataaac ctgtttatga agataaggga atcttaaaaa
     7861 gggcaatcat agctataggt aaacaagata taaatattga ctctttaatt ccaatagaca
     7921 ctgatataaa aatattaggg gcaagttcag accacatgat actagatgta agtaacacaa
     7981 aatatgatta taaattaggt gacaatttag aatttttatt aacttatggg ggtatcatgt
     8041 catctagtac aagcaaatat gtttcaaaaa aaattatagt gtagtgttta aataattagg
     8101 aggaaactat tttatgaaag agcgagaatg tagaaaagca agtctgtttg atgcaatgat
     8161 accaattact tgtttgatat tatttttaag tctaggagta ttagtgtatg gttcatcacc
     8221 tcatgtacca ctaataggag cagcagcagt agcaggactt gtggctgtat atagacttgg
     8281 atttaaatgg aaagagttag aattaagtat gtttaatagt ataaagatgg caatgcaagc
     8341 aatcttaatc ataattataa taggcgtact tataggaact tggatagtaa gtggagtagt
     8401 tccatgtatg atttactggg gactaaaaat tttatcacca aatatttttt tagtagcatc
     8461 aacattagtc tgtgcaatag tatctcttgc gacaggttca tcttggagca caatgggtac
     8521 agtaggagta gctcttttag gaattggtca aagtttaggt atgccaattg gattaatcgt
     8581 aggttctata atatctggag cttactttgg agataaacta tcacctttat ctgaaacgac
     8641 taacttagct ccagcaatgg caggaacaga tttgtttaca catataaaat atatgttata
     8701 ttcgactata ccatcattat taatttgttt agtaatttat ggtgtaattg gaatgaagta
     8761 ttcaggtcaa gcactagata taaaacaaat agaattaatt aggtctacat tagattctac
     8821 atttaatact ttatcaccag tattattatt agctccagca atagtaattg gattagttgt
     8881 atttaaagtt cctgctatac cagggcttat agtaggtgct gtattgggag tattgtttgc
     8941 agtagttttt caaggagaat ctatgaatac gatattagat gcagcacaaa atggatatgt
     9001 ttcgtcaaca caaatacagg aggtagatgc tttattatct aaaggtggga ttatgaatat
     9061 gatgtccact gtatccttaa caatatgcgc tttatctctt ggaggtattt tagaaaaaac
     9121 aggtatgctt gaagtagtag catcttcttt actaagatta gcaaagggtg tatttggaac
     9181 agtattatgt actatggtaa cttgtacaat taccaatata atagcaggtg aacaatatct
     9241 atctatagtt ataccaggaa gaatgtataa taaggaatat aaaaagagag gtatacatcc
     9301 taagatgttg tcgagagctt tagaagatag cggaacttta acttctccat tagtaccatg
     9361 gaatacatgc ggggcgtata taacagcaac attaggtgta tctgcactaa catatggtcc
     9421 atacgcttta ttaaatatta taaatccaat agtatcttta gtattaattg catgtaagtt
     9481 taaaatagca agaatagaag atgaacctga aacttgttta aattttgatg tttgataata
     9541 gataacttat gtaagaacgt aggaactagg gggggagcct gtggggaatt agtaagacat
     9601 attttagtgt gcgaaattct ataaatcagt aatcttgtga ctttagccat gggaggttca
     9661 gattagtaaa tttatagttt tatattagtc tatactttga tcttttaatt gtttgtataa
     9721 attttttata gaaaaagatt tcttttagac ttgcctaaaa attaggcaag tcttttaagt
     9781 atgttctgat tataagagca ctgtgtgaca agaatgatga gattattttt gctatacttt
     9841 cgatataaga aatggaagga ggttatgttt tatatttata tgaaataatt agttacacag
     9901 tgaattttcg tataatttgg tataaaaaga ttagaaatga aaataaagta atggaggaat
     9961 aaaatgacta gaatatccaa tataacaata gctgaacaat ctgagtactg ttttttggca
    10021 atacgaaaaa caattgactt tatggtagag ttttcagagt tttcaaaaca aagttttgaa
    10081 aaaatttcta aatatctgga ggagagagga attttatctt caggagcacc gattgtatgt
    10141 tttcataaca tggatttagc aaaattggat gttgaagtag ggtttcctgt agccacttat
    10201 atagatggga aaaatgagat tctacaaagg attgttcctg cacagaaaat aataactgca
    10261 attgatttag gaccttatga attacaagac cctactttag aagacttatt ttcatgggca
    10321 aatagcaatg gatataaact tcatggcgat atatattacc agtatttaaa tgatatcaat
    10381 cgtccagcta gtgaatatct tacgaaaatg atgttaccca ttatatagaa agttaaagat
    10441 tatcgtttgt tagttgataa ttagaagaac atttaatgct actgcataaa ttttatccta
    10501 aataatatca aggtgattta atttatgtat tctaatgtaa ctgtagataa tcaatttata
    10561 actaacttag tgtaatttag atttaattta ttattctaat gtaaccccaa tatattgtta
    10621 aggtctactt aagatgctca aaaggctatg cttaatttat tgttatataa tttgtagagt
    10681 ggtataattt agaagtgata ctatatagat atataggggg aaaatatgaa aatatatgat
    10741 atattaacac gtttttatat tcaagatata gaaaaagcca taccttttta tgagaatctt
    10801 ttaaaagaaa aatgttcttt gcgtttttct tataaagaag ttgggttgga attagcgcaa
    10861 ataggaaatg ttttactatt atctggttct gatgatgctc ttaaaccatt tatagaaaca
    10921 aaatctactt ttatggttga ttctgttgat gaatggagaa gttatctatt ggataatgga
    10981 gcagttgtag taagagataa gaaaaaagtt cctacaggat ataatatgac attgagacat
    11041 ccagatggta caattataga atatgttcaa catacaaagc aggattagtt aaaaatagat
    11101 tttactactg agcaactaat tagaatctat tcttagacgt acaatttatt atttttggta
    11161 ttaatagatt ataagtcaaa ttataatatt tcaaaataga atagaaattt atttacataa
    11221 aagagagtgt ttaaaaatga actttttagt tcacgaagca ctcctttttg tatgaaatca
    11281 aatacatcaa tatataaaac aaactgatga agagcttaaa ttaaaataaa aagattcata
    11341 acaggacccc attaaacttt aagatagggt aagtttgtaa tcatattaaa taattgtaaa
    11401 aagctgtttt tagtcgtata atataaatat ttatcattta attaaggaat ctaaaaggaa
    11461 tctaataaga agaaaataat aaataacttt ttagatttac taaatgaaaa attattgaaa
    11521 attaaaggat tagagtcttt aatcagaaag taaaaagtaa tatattaaat ttatgaattg
    11581 aaaacttaaa caacaaattt gtttataaaa tagcaagatt cattaaatat aactaagtaa
    11641 taattgatat aatgttgcat ggataaaagg agggaagctg caatgtctta caaagtaatt
    11701 aatgtcataa ctgctattga ctatatagag gagcatttat cagaaaagct ggatttagat
    11761 ataattgcta atgctgttca ttactcaaaa tatcacctgc atagaacttt cacaacttct
    11821 gttggtctaa caatgcatga ttacataaag cggagaaagt tgacagaagc agcaaaatta
    11881 ttggtatttt caaagaaacc aattattgaa attgcattga tagctggata tgaaagtcaa
    11941 caggcattca cttctatatt taaagctatg tataaaaaat ctccaaataa atataggaaa
    12001 gaacaagaat tttatccttt gcagctaaga tttgttttga aaaaagataa cctattttca
    12061 aagaatgtac tagagttgga aaaagaaatt aaacttgcaa gtatgtctga cataccatta
    12121 tggatgaatt tagtgagatt agtcatagat ggatttccaa atttgcaaga ggaagaatat
    12181 atatatcaat tagaacagta tattttagaa gaaagagctt taatattaaa acttaataat
    12241 gtcgctattg caaatatggt gtttaataga gaaactagaa gtattgattt ttttggaata
    12301 catccacagt atcgtaattc agacattgca caagtgtttt tgaagaaagt tattgaagat
    12361 ttcttgatag atacagatat ctcaattact acctttcgag agggtgacaa agctgataca
    12421 ggacatagag atatgataaa gaagttgggg tttgcagaag cagagttgct tgtggaattt
    12481 ggatatccta ctcaaaaatt tattcttcct tgtgtagaag aaagagataa tagacaaaat
    12541 taattgtaga ttaaatgcaa agtttaccac atattttttg tttgaattat tttatatata
    12601 agagtatttc tgcttttaat gtattttgct tgtaagtgta cttatggtaa taaatataga
    12661 aagatttagt tttttaaaaa aagaacttta aagctaaaaa gctaacattt aagttaaata
    12721 gcaagaatga ttaaaaactc caaaacacaa ttaagtataa tgattcagtg taagtactta
    12781 attaacaaag gaggatttaa atgtctgaaa ataaacaagt agtaaattgc gatattattg
    12841 catccaattc taaacataat atgtgtgaac atgatgtaga gttattaact ttttttccag
    12901 atgagattgc acatctaata gagataaata acaagctaga caatgctttt aagaaagctg
    12961 aaaatcttgt tgacaagctt gataaggatt atatggacgc taaaatgtat atggtaaaaa
    13021 atcgtggtga aatagaccca catgaaatgt ttcaaaatga acaaggacta aaacaaatag
    13081 ataactatgg ggcttttatg gtaaaagttc gagataagat taataaaatc aaagattctc
    13141 catactttgc tagaatagat tttaggctga aagatatgga tgatgaatct aagtattata
    13201 ttggtagatt tgcatttgat tatgaagatg agcttataat acttgactgg cgttctccaa
    13261 ttgccagtat gttttatgat tatgaaatcg gaaaagctgg atatgatgct ccaataggat
    13321 gggtggatgg agaaataaca agaaaacgac aatttaaaat taaaaatgga aaattggaat
    13381 atgcactaga aagttccatt aatatacaag atgatattct tcaaaaagaa cttagtcaca
    13441 cttcagatga aaaaatgaag tcaatcattt caacaataca aaaggaacaa aatcaaatta
    13501 ttagaaatga taaagctgat acactgatta ttcaaggggt agctggttct gggaaaacat
    13561 ctatagcatt acacagaatt gcttttcttc tttatcgatt taaagataaa atatcagcaa
    13621 ataatgtaat tatcctatca cctaataaag tctttggaga ctatatttcc aatgtccttc
    13681 ctgagcttgg agaagaacca ttatgtgaat taagttttga aaatatagca gaggtacaat
    13741 tagatagagt tattaatttt gaaagtgaga aagacccatt agaaataaat gatgctaaat
    13801 ggtctgaaag agtgcgtttt aaatctacac ttgattttgt aaaacttatt gatgattata
    13861 taaaacaaat gccaaataag atttttattc caaaagacta cacttttgga agttttacag
    13921 ctaaaagtga ctggatacag agtagatttg aggcatataa cagatatcca gttaagaaga
    13981 gattagaaaa ggtagctgaa gacattcatt ataaatttga atcagataat attatggaag
    14041 aagatttacc aaaggtaaaa agtatactta aaagtttgaa tggaatgttg acaataaaaa
    14101 atactcttac actatataag gatttcttta aacaaatgaa tatatcaaac atgtttgtga
    14161 tggcagaaaa gaaaacactt gaatggtcag atgtataccc atttatatat attcatgctg
    14221 catatgaagg tatacaagaa gataagatta taaggcatat tgttattgat gaaatgcagg
    14281 actatacacc tattcaatat gctgtaataa acttactttt taagtgtaaa aagactattc
    14341 ttggagattt tggacaattg gttaatccta atcatactca tacattagat gatatgaaac
    14401 agctatataa tgatggggaa ctagttatgt tgaacaagag ttaccgttct acttttgaga
    14461 ttattaattt cgccaaaaag attcaagata tatcttcatt agagcctatt gaacgacatg
    14521 gtgaagaacc ggctttatta aaatgtaata acgagcaaga tgaaataaat aagataaaaa
    14581 tagaaattga agaatttaaa aagagtgata atgcgactct tggtattata ttaaagacag
    14641 ataatgacgc taaagctgta tatgatgcgt taagagaaga atatagtgtt cacttaatat
    14701 cttctgaaag ttctagcttt acaaaaggag tatccataac ttctattaaa atgtcaaaag
    14761 gtttagagtt tgatgaagtt attgtacctt cagtaaataa taaaacatat tatagtgatt
    14821 atgaccgcag tttgttatat attgcttgta cacgagctat gcacaaactt aagttaacat
    14881 atgttggtac attaactcaa cttataagca tgtagattat atcttatatg catttattat
    14941 cgtaaatata aattgggaca aagattcatc aataaatcaa caaaaaataa tacttataaa
    15001 tttataaaaa atgaaagcta taaattacat ttgaaatttt ataaaatttt gtgatttata
    15061 gcttttataa tattaatttc ttttaaaata aagttaaact ttaaatttat gaaataaaac
    15121 aaagtagtaa tatatatgta atagttctta attttttaat attagtatta tattatctat
    15181 atatttattg attttctaag gaaatctgat gttatagtgc aacctctttc aaacatttct
    15241 agtggtgtac cttcaaatac gacctgacca ccgtttttgc caccatcagg accaatatca
    15301 attacccagt ctgcttgttt caatacatcc aaattatgtt caattataat tacagtatta
    15361 cctttatcaa ctatatgctc aaaaatcaac ataagctttt caatatcaga accatgaagc
    15421 cctgtagttg gctcatctaa aatatatata gtaccttttt tgttgaggtt tttagctaac
    15481 ttaattcgtt gacgttctcc tccagatagt gtgctaagtg gttgacctaa ggtcatataa
    15541 ggtaaaccaa catctttcat tgtttgtaag attttacata gcttcttttc tgtaaaaaat
    15601 tcaagagatt catctactgt catagatagt acatcaacta tacatttacc tttatataat
    15661 tttgacaaaa cttcttcact atatctagta cctttgcaag cttcacatgt attaataata
    15721 gggtccatga atacaagctc tgtaacaagt actcctttac catcacaaac aggacaagct
    15781 ccctttgaat taaagctgaa taaaccttct tcaacattat tttctttagc aaatactttt
    15841 cttatctcat caaagaaacc aagaaatgta gcaggcatag aacgatttgt agctgtaact
    15901 gggctttggt cgattttaat aacatctttt tcgtattttt gtgcaaaaac acgagagatt
    15961 aaagtactct tgccagaacc tgctactcca gtaacgacag tcataattcc aactggtaaa
    16021 tctacatcaa tatgttttag attatttatg caagcatctc taattggaag ggtacctttt
    16081 ggatgacgag agtcagtttt tataggtaat gatagattca ttgcatttga agtaagagtg
    16141 tttgctaaaa gtaatttatc ataagagcct tggaatacaa catgtccacc ctgagtacca
    16201 gcacttgggc caatatctat aacttcatct gctatggaga taacatcttt atcatgctct
    16261 actacaagga cagtatttcc tctatctcta agttttttta atagctgatt catacgatgt
    16321 acatctcgtg gatgcatacc agcactaggt tcatcgaaaa tataagtcat acctgtgaga
    16381 ctgctcccca tatagcgtac aagttttaat ctttgagctt ctcctccaga tagagatgat
    16441 gattctctat ttagatgtag gtaagggaga ccaatatcaa tcattctttt taaaccatca
    16501 gtaagagtct ttattacagt ttcagttctt ttatctttta tttgtaacaa taaatcataa
    16561 aggtcagtaa gttccatatt gcacatatct gcaatcgaat atcctaaaat attacagtta
    16621 agagtttctt tatttaatct ttttccatga catacaggac attccttatc tgttataagt
    16681 ctttctgatt tttcttttgt gtgtttgctc atattgctaa tatctcgatt tagatatgtt
    16741 tgattgtatt tgttaaaaat accctctatt tttggatttt cttgttttcc atctttattt
    16801 tttgaaccat aaagcaataa attatattct tcaaaagaat aatcttttat aggtttatct
    16861 aaatcaaaaa gtccagactc tgcatactgc ttccagtacc atgaattagg tttaaataaa
    16921 gagtcattta tacatcctgt attccatgaa ttttcaggat ttataataga ttcaatattg
    16981 attactttaa ctctaccaat cccagaacaa tttttacaca taccatttgg gtcattaaaa
    17041 gaaaaatatg aagttgttcc tatgtagggt tctccaattc tagaaaataa taatcttaat
    17101 gatgaataca aatcacttat agtgccaact gtagaacgtg cattgccacc aagaggagtt
    17161 tggtctacga ttacagaagc agttaaatta tcaatcagct ccacctgagg ttttctatat
    17221 ttaggtagcc tatttctgat aaatgaagaa tatgtttcat tcatctgcct ttgtgattct
    17281 gctgcaattg tatcaaatac gatactggat tttccagaac cagaaactcc agtaaaaaca
    17341 acaattttat ttttaggaat tttaaatgat atatttttta ggttattttg tgtaagtcct
    17401 tttattataa tatttgacat gaatgttaaa tcctttctga gtacatttta gtaaagcaat
    17461 tatattatgt aaccatatta taataaaatt aaaatctact ttcttgatat attctgctat
    17521 gttatagaag gtatttctaa atttaaccaa tcccatacaa tctgaataaa tgatagttga
    17581 taatagataa aaaagtatat aatagatact cgtaatttga taagtacaaa gataattata
    17641 caattaaaac gacttgacat ttaaaactta ctattgtaat atttaaatat tacaatagta
    17701 agttttaaat agagaataaa taattacctc acaaactgaa aggaggacat aatgatacta
    17761 atagttatat tgagtatatt catcatagcc tcaatcaaca taattatatt aggtaaagaa
    17821 aatataagag atatagttat ccctttaact ctatttattg taagtgattt tttatttttg
    17881 tgtggaaata taatatgtag gggaggagta atgctactat ttataggttt atttgcaata
    17941 ccattgatgc taattgcatt tatatggatt tgtgtacata tgattaagct ttactataaa
    18001 aaagatttaa ataaaagaaa aatgattgga tgtatattta gtatctttat aagtataggt
    18061 actatattta ttcctagtcc aagtgaaagt tttagattta aactttatga aaaagattat
    18121 agtgtagtag ctaaagctat tttgaaagtt tatgatgaaa aaaagttaag ctctgaatct
    18181 gtcttttata ttcaatctga taaaaatgaa ttggagaaga tattttctaa agaagtaata
    18241 aaaaaaatga aaaaactaaa cagaaattta gatgtagtta aaatttctgg gaatagaaat
    18301 ttagtctatt ttttctttgg agctgaatta caatctattg atggtattgt gatttttaaa
    18361 gatttaaaac atgttaaacc aaagttagat tttataaata gaagtattca agctcctaga
    18421 ttggagtata ttacaaataa tgtatattat tttaaaggtg aactataaac ttatgtagag
    18481 gagatggtat aaagtatgaa aagaaaaaat aattttattt ggatagttat tcttctagtt
    18541 gttgtagcta tgggagtata ttatgtagga agaaagcata acaacacaaa acaaaaacat
    18601 gataagaatc atataaactc tgaattaaat aaatccaaag ttgaaaacaa tgataaaaac
    18661 aaaaaagaaa aaattaatat ggttgactat agtgactgtt ttgaaggtat tagtggagga
    18721 gctatttttt ataatactaa aaataaagaa tataatatat ataacaaaga attaattgaa
    18781 acaagaaggt caccatgttc tacttttaaa atagtttcaa ctttaattgg tttagaaaaa
    18841 ggggtaataa actcaaaaga atcagttatg ggatatgatg gtacagagta tccaaataaa
    18901 aattggaata aaaatcttag tttagaagag gcatttaagg agtcttgtgt ttggtactac
    18961 aaaaaattga tagataaagt tgatgctaaa tctgtccaaa acattttaga tgatttaaag
    19021 tatgggaatt gcgatattag cgaatgggaa ggagatttaa aaaatggaaa gggacattta
    19081 aatggatttt ggttagaatc ttcattacaa atatctccaa aagaacaagt gcaaacaatg
    19141 gcaaaaatat ttgagggaga tacaaacttt aaaaaagagc atattaatat tttaagagat
    19201 attatgaaaa tagatgtaaa tgataaaaac ataaatgtat atggaaaaac tggaacagga
    19261 tttgatgaaa aaaataaacg tgtggatgca tggtttgttg gtatgctaga gcgtgaaggt
    19321 gacacctatt attttgctat taaatcagat gattccaata aagaaattac agggccaaag
    19381 gtaaaggaaa ttgcaattaa tataattaag aaatattata gtgtaaggga ataaacttaa
    19441 ttttatttgt aaatattatt tacctatagt ctgtaatatt tattaaataa ttttactaaa
    19501 ttgagtctat atagagcaaa actcctttag tacataaaat acttgctatt gattattaaa
    19561 ttgaatacct atagatttaa agttaatacc ttttaaagac tatgtaatat attaaaagta
    19621 taaataaaat atattgcata gtttttgttt tatattataa ttttaaataa tgtgcaaaaa
    19681 ctaaacaata
//
LOCUS       DACTGW010000053        19392 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.053, whole genome shotgun sequence.
ACCESSION   DACTGW010000053 DACTGW010000000
VERSION     DACTGW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19392
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.053"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            167..688
                     /locus_tag="I9573_001508"
     CDS             167..688
                     /locus_tag="I9573_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4796134.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSARTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVVDNDKTK"
     gene            722..1498
                     /locus_tag="I9573_001509"
     CDS             722..1498
                     /locus_tag="I9573_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT4796135.1"
                     /translation="MKFHEFGDKNKPHIMLIHGGGNAWWNYLRQAEVLSNNYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKGYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1841..2794
                     /locus_tag="I9573_001510"
     CDS             1841..2794
                     /locus_tag="I9573_001510"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HAT4796136.1"
                     /translation="MINVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2812..3831
                     /locus_tag="I9573_001511"
     CDS             2812..3831
                     /locus_tag="I9573_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HAT4796137.1"
                     /translation="MFKKCIKVVTLTFILACILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3862..4113
                     /locus_tag="I9573_001512"
     CDS             3862..4113
                     /locus_tag="I9573_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796138.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4395..6200
                     /gene="lepA"
                     /locus_tag="I9573_001513"
     CDS             4395..6200
                     /gene="lepA"
                     /locus_tag="I9573_001513"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAT4796139.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6351..7688
                     /locus_tag="I9573_001514"
     CDS             6351..7688
                     /locus_tag="I9573_001514"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAT4796140.1"
                     /translation="MINIEIPKKVDYIIKELETNGYEAYVVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLISTEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILRRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            7921..9234
                     /locus_tag="I9573_001515"
     CDS             7921..9234
                     /locus_tag="I9573_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT4796141.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTIISLGGIFITLLGLPVYYYMKKR"
     gene            9406..10581
                     /locus_tag="I9573_001516"
     CDS             9406..10581
                     /locus_tag="I9573_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAT4796142.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMKLY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10684..11703
                     /gene="hrcA"
                     /locus_tag="I9573_001517"
     CDS             10684..11703
                     /gene="hrcA"
                     /locus_tag="I9573_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HAT4796143.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11733..12353
                     /gene="grpE"
                     /locus_tag="I9573_001518"
     CDS             11733..12353
                     /gene="grpE"
                     /locus_tag="I9573_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAT4796144.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12445..14292
                     /gene="dnaK"
                     /locus_tag="I9573_001519"
     CDS             12445..14292
                     /gene="dnaK"
                     /locus_tag="I9573_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAT4796145.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLADEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14503..15657
                     /gene="dnaJ"
                     /locus_tag="I9573_001520"
     CDS             14503..15657
                     /gene="dnaJ"
                     /locus_tag="I9573_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAT4796146.1"
                     /translation="MSTKRDYYEVLGISKDAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16623..17558
                     /locus_tag="I9573_001521"
     CDS             16623..17558
                     /locus_tag="I9573_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAT4796147.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     AEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKDYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17594..18967
                     /locus_tag="I9573_001522"
     CDS             17594..18967
                     /locus_tag="I9573_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4796148.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEGNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19155..>19392)
                     /locus_tag="I9573_001523"
     CDS             complement(19155..>19392)
                     /locus_tag="I9573_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HAT4796149.1"
                     /translation="IDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTLVYR
                     LEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
BASE COUNT         7681 a         2143 c         3527 g         6041 t
ORIGIN      
        1 aatataacta atatgttaga aggttatatg taattaatct cagtgtttat atagaatagc
       61 atttgtagca tttattatag ttgtaattac tacttatatc atagacttga atagtctaaa
      121 aaaatagact ataatatttt tatagactaa agaaagggct gataagatgg acatagcaaa
      181 actaatttta aatcctgcaa ggcttcgtat cttacaatat attcgacttc atggcagtgc
      241 tcgcacatct gatattgtaa agtatttaaa tgatattcct cgtgcaacag tttatcacca
      301 tgtaaaaatt ttagaagaga acaatatgat tgaagtaatt aaagaaaatc gtgtgcgtgg
      361 tactattgaa aaggtttatg cactaaagga atatacaaca tctatggaag gagagacctt
      421 cgttgcactt tctacagcat tttatgtagg tctgatgcag gaaatgaatg aatactttag
      481 cagaaaagag caagaccata aaaaggataa tgtatttttc tcttcagcat tactatatat
      541 gagtgataat gaatatgaaa atcttcttaa aagtatagct gatttattga aaccatatat
      601 agaacaaaag cctaaatcaa atctaaaact tagaaaactt tcaattatat cttctccacc
      661 tgtggttgat aatgataaaa caaaataaca tgaatcttat ttttataaaa ggaggttgta
      721 tatgaaattt catgaatttg gagataaaaa caaaccacat attatgttga ttcatggggg
      781 agggaatgcg tggtggaact atttgcgaca agcagaagtt ttgtctaaca actatcatgt
      841 gatacttcct acattggatg gtcatggaga agaatatgaa acaacataca tttctacaga
      901 ggatacagca gataagttga tatcatatat agatgaaaat tgcagtggac atcttttcgc
      961 tttgtgtggg gtatcacttg gtggtcaaat tgtcatggaa cttttatctc gaaaacctaa
     1021 tattactaaa aaagcaatta ttgatggtag tatttgctac ccaagacctc ttatggcaag
     1081 gttttgtatt gctggtattc gattttttgg aggacttttg tttagtgaga aggcttgtcg
     1141 ctttcaaatt gctatgatgc caaaattgtt acctgaaaat atgcaatatc cagatgaaat
     1201 aaaagcgtat tatatgacag atatgccaca tgtaagaaaa gaaacattct ataatatgta
     1261 tcgtacttat atgatgaact atacacttaa agagaatgtt aaaaaaacca cagcacaagt
     1321 aatgtactgg tatggagaaa aagaaatgaa gtgcgttaaa aaatctgcaa aaatgtttca
     1381 gtcttatgta ccctcttgtg agatttatga agctaagggc tataatcatg ggtatttatc
     1441 gctttatctt ccaaatgaat ggttggaaat tgcagaacca tttttccaaa aggaataaag
     1501 gtgttaggtg attattgtat aattattgct gtttttttat atagtaaata aattaggtat
     1561 aaatataaaa aagttatttt agttaatata ttagtgaaag cttaatagga aaattataaa
     1621 tagattaaag gtagcgccta tataaatagt tgctaccttt ttttgcttgt aaataaatga
     1681 tagataagaa agtttactga caaatcactg actaaaccca attttcttac attgaaagcc
     1741 ttaattttac ttgttttaaa taaatttaca atttcttgtt ataaaatact aaattttgtt
     1801 aacaattata attagcattt aatttatact ggaggtttat atgattaatg taagaacaga
     1861 tttagcttta gaagctagtg aaatgtgtga aaagtctcaa gaaggaagta gtattcctgg
     1921 tgtaaaaata gaaactaaag aattagaaaa ttgcattgta actaaagttg aagtaataga
     1981 caggcaaggc gcagagataa tgaataaaga tataggaaag tatataactc tggaaagtaa
     2041 tcttatgaag tttgatgatg atgagtcgag agaagaaatg ataagttatt taaaagagga
     2101 gttagtagat atatttggcc aagacaaaaa taaaaaaacc ctggtaattg gtcttggaaa
     2161 tagaaatatt acttctgatg cgttaggacc aaagagtgta tcaaaaacac ttgtaactag
     2221 acatcttttt aaaaattata ataaagacta tgatgatgat ttcactgagg tatcagcttt
     2281 aagcccagga gttatgggtg ttactggaat agaaactagt gaaatagtaa agtcattggt
     2341 tgaaaaagta aaaccagata gagtagtagc aatagacgca ttggcttcaa gaaaaatgga
     2401 aagagtaaac tctactatac agatatcaac agcaggaata tcacctggtg gaggtgttgg
     2461 caatactaga aaatctctaa ctaaagaaac tttaggcgtg gatgttattg caataggcgt
     2521 tccaacggtg gttgatgcag caactcttac aatagatgtc ttagatatgg caatagacaa
     2581 tttaatagct caatcagaag aaacagaaag tttttatgaa atgttgaaaa aacttaaaga
     2641 agaagaaaaa tatcatctaa taaaagattc gttagaccca tatgacaaga acttaatagt
     2701 aacaccaaag gatatagatg atacaatcga aaatctatct ataataataa gtgaaggctt
     2761 aaataggtct ttacatccag gtagattggt ataaaataag gaggagcaat tatgtttaaa
     2821 aaatgcatta aagtagtaac cttgactttt atattagcat gcattttacc aggaaaatca
     2881 ttagctttga atcaggatga ttttttaaag tttttagtaa attcatctta tccagaagct
     2941 aaagtcgaag gaaatgatac agaaaataaa aagaataata aaaataaaga aacaagcaaa
     3001 gaaaataagg aagaaagtaa agaagaaaac actaaatcta aagatgcaag taaagttgat
     3061 aataaaaaag agagtgaaaa agaatatata aaactttatg ttggtaaaga aaatgttcca
     3121 gatattgagt caaaaaattc agacacaact gaaactaaca caacatctag ttcagattat
     3181 aaggatgatt taagagtaac caaagaaaat ccaagaattt tgatatatca tactcatgga
     3241 tgtgagactt attcaaattc acctgatgga aattatcatt caagagataa gaaaaattca
     3301 gtaatggaag taggaagtgc attaactagt gctttagata gcaaaggatg gggtgtagtc
     3361 catactacta aataccatga ctatccatct tataataact cttatgcaag tagcttaaaa
     3421 acaatacaaa gtatattacc taaatataac tcagtggata tagcgataga cttacataga
     3481 gatgctaggg atttaactaa tccagccact aaggaaaaag accatttaaa atatacaact
     3541 atgataaatg gagagagagt atcaaagttc ttctttgttg taggaggtaa aaatacaaat
     3601 agaaaacaac ttagagcttt agcagaagat ataactgctt ttgctgagaa aaagtatcct
     3661 ggtctagtat ctccaatagt agaaaaagac tatgctagat tcaatcagtt tgcagttaag
     3721 aatcatatgt tagtagaaat agggaataac gcaacaagtg tagaagaatc taaggctact
     3781 acaaagtatt tagccgagat tttagatgaa tattttaaac aaaaaaatta gagctttaag
     3841 ttataattag gagcatgcta aatgagccga ttagaaaaaa atatagaaag taaaagtaaa
     3901 ctaagaaaat acaagtatat tatgagaatc ttatttttga ttactatgat tataggtaca
     3961 tcagtttgtg tatttatagt agacagtaat gcaaaaagta tgctaggaat gaaacaatat
     4021 gattttccaa gtattaaatt tgatataaca aaatttgatt ttgacatacc aaattttgat
     4081 atacatgaaa tcataaaaaa atttaataag tagtaataag tgaataaggt ttattgatta
     4141 aaataagcct tattttagtt atatattttt aatgtataaa ataaaaattc attaactgta
     4201 ttttatagga tttactttaa gtttacgata ttatataata tttagtgtta gacttgataa
     4261 tccacatata atgtggataa ttttgtttag tgccaattag tttatattca aaaattttaa
     4321 atttggtata taaagagttt ttagataatg taaaataata actataagaa taatcaagag
     4381 gagggaattt taaggtggac aataaacaaa gtagaactag aaattttagt ataattgcac
     4441 atatagacca tggaaagtct accttagctg atagattaat acaacaaact gggcttgtta
     4501 gtgaaagaga catgaaaagt caattacttg acaatatgga ccttgaaaga gaaagaggaa
     4561 tcactataaa gcttcaaaac ataagattaa tgtataaagc gaaggatgga aatgaatatt
     4621 atttaaatct tatagataca cctggacatg tagactttaa ctatgaggta tcaagaagtt
     4681 tagctgcatg tgaaggagca ctattagtag ttgatgcagc tcaaggtgtt gaggcacaaa
     4741 ctttagccaa tgtttatctt gctatagacc aagatttaga aatattacca ataataaata
     4801 aaatagattt accaagtgca agaccagagg aagtaaaaaa tgaaatagaa gatttaatag
     4861 ggcttgattc aagtgaagct cctcttattt cagctaaaac tggtttaaac atagaagatg
     4921 ttttagaaga tattgtaaaa aatgttcctc cacctaaggg agataatgaa gctccattaa
     4981 aggcattaat attcgattct tactatgatg cttacaaagg tgttgtagct tatgttagag
     5041 tatttgaagg aactgttaaa aagggtatga caataaaaat gatgaatacc aataaaaagt
     5101 ttgaggtaac tgaagttggt gttatggctc caggtcaaac tgaacttagt gaattatcag
     5161 caggtgatgt tggttatatt gctgctagta taaaagatat aagaagttgt cgtgttggtg
     5221 atacaataac agattcaaat aatcctacag aagaaccttt accaggatac aaaaaagcta
     5281 ctccaatggt atattgtggt atatatccag gagaaggaga aaagtatgag aatgttagag
     5341 atgctcttga aaagcttcaa gtaaatgatg ctgcacttga atatgaagct gagacttcag
     5401 cagcactagg atttggtttt agatgtggtt tcttaggtct tttacatatg gagataatgc
     5461 aagaaagact tgagagagaa tttaaccttg atataattac tacagctcca tctgttatat
     5521 atagagttac aaaaatggat ggagaggttg taatgataca aaatcctgca aacttaccag
     5581 aaccatctga gataaagatg atagaagagc ctatagtaaa aggagatata atagttccta
     5641 aagactatgt gggtgttgtt atggagcttt gccaggaaag acgtggaaat atgttaaata
     5701 tggaatatat agatgagaga agagtaatgc ttcactatga ccttcctctc aatgaagttg
     5761 tttacgattt ctttgatgct ttaaaatcga gaactagagg ttatggttct cttgattatg
     5821 aggttaaagg ttatgtagca tctacacttg taaaacttga tatattaatc aataaagaac
     5881 aagtggatgc acttagtttt attgtccatg agacacgtgc tttccctaga ggaaaggcaa
     5941 tgtgtgagaa attaaaagga gaaattccaa gacatcagtt tgctatacct atacaagctg
     6001 ctgttggtaa taaggttatt gcaagagaaa caataagcgc tctaagaaaa gatgttcttg
     6061 ctaaatgtta tggtggggat atatctcgta agaagaaact tcttgaaaag caaaaagaag
     6121 gtaagaagcg tatgagacaa attggttctg ttgaagttcc tcaaaaagca tttatgtctg
     6181 tacttaaact agatgaataa attcttataa atttagataa atatatttaa aagctagagt
     6241 gattttagaa ttatttctaa attaatctag cttttttgtg gtatatttta agtaaactca
     6301 tagagaatat aagtaagaat atattaaaga agaataaaaa ggtgatattt atgattaata
     6361 tagaaattcc taaaaaagta gactatataa ttaaagaatt agaaacgaat ggttatgaag
     6421 cttatgtagt tggaggatgc gttagagatt gtttattaga aagagttcca aacgattggg
     6481 atataacaac tagtgctaga ccagaagtgg tagtagaatt atttgaaaaa acaattccaa
     6541 ctggtataca acatgggact gtaacagtta tgattgaaca tgaaccattt gaagtgacaa
     6601 cttatagaat tgatggaaat tatagtgatg gtagacatcc agattcaata gaatttacaa
     6661 ataacatagt taaggacttg tcaagaagag attttactat caattcaatt gcatacaatt
     6721 caaagacagg attagtcgac ccatttaatg gctatgaaga tatacaaaat aagtatatac
     6781 gttgtgttgg aaatccagta gatagatttg aagaagatgc acttagaatg cttagggcag
     6841 ttagattttc agcacaactt aattttaaaa tagctgaagg tactaagcaa agtattcata
     6901 aaaaagcaga tttgataaaa aatgtttcta tcgaaagaat ccagacagag tttaataaaa
     6961 tattagtatc tgattcatct aaattaaact tattaaaatc aacaggtttg ttgaaattta
     7021 taattcctga aatatgtgaa ttagaagatg ttactcaaca caatccatat cacatctatg
     7081 atgtacaaaa acatacttta atatctacag aagtaattga agatgaactg tatttaaaac
     7141 ttacaatgtt gtttcatgat ttaggtaaaa aagtaactaa aacaactgat aaaaatggtg
     7201 ttgaccattt ttatactcat agtagagaat ctgtaaagat tgcaaagaag attttgagaa
     7261 gacttaagta tgataattat actattaata aagttttaat attaatacaa tatcatgatt
     7321 atagaataga acctaaaagg aagataatta aaaagttatt aaataaactt gaagatgttg
     7381 aattatttga agatttaata aaagttaatt gggctgatac acttgccaaa aacccaaagt
     7441 atgcaaaaca aaagatatta aatcttattg agtgtgaaaa agaatttaaa catataataa
     7501 atcaaaagga gtgctttaat cttaaagatt tagctattaa tggaaaagat ttgatatcta
     7561 taggtataaa accaggtaaa gatataggac atattttaaa taagatgctt gaaatagtca
     7621 taaataatcc agaattaaat gaaaaagaaa tactaaaaga aaaagcatta aatatatata
     7681 cattttagat tcaaatataa aaaatttaat tactttaaaa aattatagaa gaaaatactt
     7741 atttagataa aataaataat ttttagagaa tattttagaa gttacgattg tatgattttt
     7801 aaacgcatag catttactct aggactatag actaatataa aaaaaaactt tgggaatact
     7861 aaaaccataa atttataaaa ttaatatttt atatactgta aaacagtgag gagggacagt
     7921 atgcatactc aactacaaaa gactattggt ctttcagcgg ctctttctac tgttgtaggt
     7981 atggttatag gttcgggagt tttttttaag ccacaagcaa tatacactac aactaatgga
     8041 gctccaggtc taggtattat agcgtggctt ttaggtggtt ttataacaat aactgctggg
     8101 cttacagcta cagaaatttc agctgctata ccaaaaactg gtggtatgat gatttatatc
     8161 gaagaaatat atggagaaaa gttaggcttt ttaactggat ggatgcaaac tgtattattt
     8221 ttcccaggta catcagcagc attaggagta atatttgctc aacaagcatc tgaactttta
     8281 ggaatgagcc caaataatat ggcaaatgta cttcctatag caattggagt tatactattt
     8341 ttagcgttat taaatataat aggttcatcc ttaggaggaa aagttcagac agtagccact
     8401 attggaaaga tgataccttt aattttaata atagtctttg gatttattaa aggtcaaagt
     8461 agtgaagtat taaatccatt tgtaggagat ggtgtaaatg catcaaatgc acttggacaa
     8521 gtattaatag ctactttgtt tgcatatgat ggttggataa atgttggagc tatatcagga
     8581 gaaatgaagt ctccagagaa agatttacca agagcaatag ttggtggttt atcattagtt
     8641 atggcagttt atattataat aaatatagct tatctttggg tagtaccagc aagtgaatta
     8701 gcaacagtta cttccccagc tacattagta gcaacaagat tgtttggaaa tataggtgga
     8761 aaagtaataa ctgtgggtat attaatatca gtatttggga cattgaatgg atatttatta
     8821 acaggttcaa ggatacctta tacattagct gaaatgggaa ctttaccagc atctaaaact
     8881 cttttaaaag taaattctgg aggttctcct gtaaattcaa tactacttat aactgtgtta
     8941 gcctgtgtat atgcattatc tggtcaattt aatcttttga cagacttaac tatattttca
     9001 atatgggtat tttatgtatt gacttttata ggtgttatga ggcttagaag acaaaagcca
     9061 gatttacaca gaccatataa agtcccttta tatccaataa taccaattat agctatacta
     9121 ggtggactgt ttgtaataat aaatcaaata ttgacttcaa caattatatc tttaggtgga
     9181 atatttataa cactattagg tttaccagtt tattattata tgaaaaaaag ataactactt
     9241 aatttaagtt agataaattt gggtaaaggt gaaaattaac ttttatccaa attttttata
     9301 ctgttataca atataagcat tatatattac ttgatgtgtt attttgtttg atttataatt
     9361 tagtaattga tgctttaaga taatttaaga aatgaggaat aaaaaatgct aggactttat
     9421 gtacatattc cattttgtgt gaaaaaatgt aagtattgtg attttaattc ttataaaatg
     9481 gacatagatt caaaaaaaag atatatagaa gacttaaaaa tagaaatgaa attatatagt
     9541 aataaattat ataaagataa taaatataaa aataaagaat gctgtagtct aaataaaaat
     9601 gacaaaataa cgagtatatt tgtaggggga ggtacaccta gtatactaac atctgatgaa
     9661 ataagagaag tatttataag tataaaagaa atgtttgata tagatgagaa tgcagaaata
     9721 actatagagt gtaatcctgg gacattaaca ttagaaaaat taaaaactat gaaagagatt
     9781 ggcataaata gacttagtat agggctacag gccattcaag aaaagcattt gaattttata
     9841 ggaagaatac atacttatga agaatttgag aaaaactata aagatgcatt aagtgtaggt
     9901 tttaaaaata ttaatgtaga tttaatgtac agtcttccaa atcaaactct atgtgactgg
     9961 aaggaaactc ttgaaaaagt tgtacactta aatccaacac atatatcagc gtattccctt
    10021 atactagaag aaggaactga actatataat atgtatgaaa gtaataaatt tgagcttata
    10081 gatgaaaatg tagatataga aatgtatgag tatacaataa actacttaaa gtcaaaagga
    10141 tataatcaat atgaaatatc aaattactca aaagaaggct ataattgtga gcataatatt
    10201 ttatattggg aatgtgaaca ttatataggg attggagcag gagcttctgg ctatattaat
    10261 gagaacagat acaataatgt agaatcttta gaagattatc atttaagttt ggtaaaaaga
    10321 gaaaaaccaa tacaagaaaa tgaaattctt tctgagaaag atatgataga agaaaagata
    10381 tttatggggc ttagaatgaa taaaggcata aaatttgaag attttaaaaa aaaatttgga
    10441 atagatttta gagagaagta taataagcaa atagaaatgc tattagctag aaagcttata
    10501 aatcagagct ttgaaggtat acaattaact caaaaaggaa gagaaatatc taatagtgta
    10561 tttatagaat ttatggaata atttttaagg tgttgacaaa ataaatttat aatggtatat
    10621 aatatatata atctttttag cactcgaact tagtgagtgc taacagctag gaggtataat
    10681 aatatggaat taaatgaaag aaaattaaat atcctcaaag ctatagttaa agattatata
    10741 gaaacagctg aagctatagg ttctagaaca atatctaaaa gacatgattt aggtgttagt
    10801 gctgctacca taagaaatga aatggctgac cttgaagaat tagggtattt aattcagcct
    10861 catacttctg cgggtagagt tccatctgaa aaaggctata agttatatgt aaactctctt
    10921 atgagtaaaa gcgaattgga tgataatgat aagattctta ttgaacaatg tatgaatcat
    10981 aatataaatc atataaaaga attgattcat gaaacctcta agctattatc gcaactaaca
    11041 aactatacaa ctgtagcagt tactaagagt ttaataaatc aaagtgttat aaagcacata
    11101 cagttggttg ctatgaatga caataatatt ttacttatag ttgtaacaga taaaggggac
    11161 ttaaaaaaag ctaaccttac aactaatgta tatttagatc aatctaagtt aaatttgatt
    11221 tctgataatc ttacaaggaa gcttttaggt aaaagtataa cagatttgga tgataatcta
    11281 attgctttta ttaaatacga aataagtgag tattctggac ttatagatga gcttttaaat
    11341 gctttgaact caaatatgaa agaagaagat ttttctctat cattaaatgg agctactaac
    11401 atatttagtt atccagaatt taatgatgta ttaaaagcaa agtcattttt aaatatgttg
    11461 gaaaaaaagg aaacaatagc tgacataatt aaatcaaagg gaatacaaaa agacaatcta
    11521 aatataataa ttggtagtga taatgattgt gaactggcac aggattgtag tatagttaca
    11581 gcaacttata atgtagatag agacttggtt gggcgaataa gctttatagg ccctaccaga
    11641 atggattatg ctagaattta ctctataata aattatatga gtttattaat taatagaaag
    11701 taaagaaatt ttaaattgcg aggtgtaaag tcttggataa aaaaaatgag caacaggaag
    11761 taagagaaga aaatgatact tctattaacc aagaatcgga aactcaagta gagttagaag
    11821 aggaagttgt taatgaagaa tgtgaaacat caagtgaaaa aactgatgaa aaagaagttg
    11881 atgatgaaaa tgtaacagat ataaattcta agttagcaga aaaaaaattg caggatgagt
    11941 tggatgaatt aaatgataaa tatcaaagac ttcaagccga atatgcaaac tacagaagaa
    12001 gaactcaaca agagaaagag acaataggcg tatttgcaaa tgaaaaaata ataactgagt
    12061 tgataccagt tatagatagt atggaaagag ccttagatgc atgtgaagat aaggaagata
    12121 ctatgtataa gggtataagc cttgtacata aacaattaat agacactttg gtaaaatttg
    12181 gagtagaaga aatagaagca gaatctaagg aatttgaccc taatctacat ctagcagtta
    12241 tgcaagaaag tgtagatgga gttgaagcaa atcaaatagt aatggtactt caaaaaggat
    12301 ataagctagg aactaaagtt gtaagacctt caatggttaa agtttcttgc taagtataaa
    12361 ttaaaggtta aattatttaa cctaatataa aatattaaaa taagtaaaac ttaattatat
    12421 aattactcga ggaggatttt agtcatggga aaaataatag gaatagattt aggaacaaca
    12481 aattcatgtg ttgcagtgtt agaaggtgga gaagcacaaa taatagcaaa tagtgaaggt
    12541 atgagaacta ctccatcagt agtagcattt acaaaagatg gtgagagaat agttggagaa
    12601 cctgcaaaaa gacaagcagt tacaaatgca gataaaacaa taacttctat aaaaactcat
    12661 atgggaactg attataaagt aaatatagat ggaaaatcat atactccaca agaaatatca
    12721 gcaataatat tacaaaaatt aaaatcagat gcagaaagtt acttaggaca aacagtaaca
    12781 gaagcagtta taacagtacc agcttacttt acagatgctc aaagacaagc gactaaagat
    12841 gctggtagaa tagcagggct tgatgttaag agaataataa atgagccaac agcagcagct
    12901 cttgcttatg gtatggataa attagaccaa gaaaagaaaa tattagtatt tgacttaggt
    12961 ggaggaactt ttgacgtatc tatacttgaa ataggagatg gaacttttga agttttagct
    13021 acagctggta acaatagatt aggtggagat gactttgacc aaatagtaat agattattta
    13081 gcagatgaat ttaaaaaagc tgaaggtgta gatttaagaa atgataaaat ggctcttcaa
    13141 agattaaaag aagcagcaga aaaggctaag aaagaattat catcaacaat gagttcaaac
    13201 ataaacttac ctttcataac tgctacagca gaaggaccaa aacacttaaa tatagattta
    13261 tcaagagcta aatttgaaga attaactaga ggtttagttg aaaaaactat ggaaccaact
    13321 aagacagctt tacaagatgc aggactttct acaggtgata tagatgatgt attattagta
    13381 ggtggttcta caagaatacc agcagtacaa gaagctgtta agaagtttat aggaaaagaa
    13441 cctcataaag gtataaaccc agatgagtgt gttgcagcag gtgcttctat acaagctgga
    13501 gttttagcag gggatgttaa agatttatta ttactagatg ttactccatt atctcttggt
    13561 atagaaacta tgggtaatgt aatgactaag ataatagaaa gaaatactac aataccaact
    13621 aagaagtctc aaatattctc aactgctgct gataaccaaa cagctgttga tatacatgtt
    13681 cttcaaggtg aaagaagtat ggcttatgac aatacaactt taggaagatt ccaattaaca
    13741 gatataccac cagcacaaag aggaatacct caaatagaag ttacttttga tatagatgca
    13801 aatggtatag ttaatgtttc agctaaagat ttaggtacag gaaaagagca aaaaataact
    13861 ataacatcaa acactaattt atcagaagca gaaatagagc aaaagataaa agaagcagaa
    13921 atgaatgcag aagctgacaa acaaaagaaa gaaaaaatag aagcattcaa tcaagctgaa
    13981 tctactatat atcaaactga aaaaacttta aatgaacttg gagataaaat aagttcaggt
    14041 gaaaaagagg atatagaaaa agctatagca gacttaaaag ctgtaaaaga taatcaagat
    14101 gctacagcag aagaacttaa gaaagctaca gatgaagtaa tgactaagtt ccaaaaagta
    14161 tctcaagaaa tgtaccaaaa agcagctcaa gaacaacaag cggcacaagg tgcagaacaa
    14221 gcacaagaca atggacctaa agatgacaat gtagtagatg ctgactttaa agaagtagat
    14281 gaagataaat aagctatata atatgtaaca ctaaatgata attggaacat atatgatttg
    14341 tgaaaatcat atagatgtta aagatatatg gacaatacac atcataaaga atataatata
    14401 tcatagatta tataacttaa agtagaatat aagaattaat gatagcttgt agtttattat
    14461 tctacaagct atcattttgt aaaatgaagg tggtgtcaat ttttgagtac aaaaagggat
    14521 tattatgaag tcttaggtat cagtaaagat gcagaagctc aagagataaa aaaagcttat
    14581 agaaaattag caatgaagta ccatccagat agaaatcctg gagataaaga ggcggaagaa
    14641 aaatttaaag agataaatga agcttatgaa gttctatctg atgatacaaa gagaaaaact
    14701 tatgaccagt ttggacatga tggattaaat ggtcaaggtg gttttggtgg tcaaggagga
    14761 tttggcggtc aaggctttgg tggttttgaa gatatgttcg gagatatatt tggtgacatg
    14821 tttggaggaa gcttcggagg tggaagagca agaagaagag ggcctcaacg tggtgcagat
    14881 ataagacaga gtgttactat atcttttgaa gaagctgcat ttggtaaaaa gatgtctata
    14941 aaagtaaata gaagtgaaga gtgtgaagaa tgtaatggaa ctggagcaaa accaggaaca
    15001 tcaaagaaaa cttgttcaac ttgtaatgga actggccaag ttagaactgt tcaaagaact
    15061 ccatttggta atatagcaag ttcaagacca tgtagtgcct gtaatggaac tggagaagtg
    15121 attgaatctc catgtagtaa atgtcatgga actggaaata caagaaaagt taagactata
    15181 gaagtagata tacctgctgg tatagatgat ggtcaaatga taaaactttc tggtcaaggt
    15241 gaagtaggag aaaaaggtgc tccaagagga gacttatata ttgtagttaa tgttaagtca
    15301 catccactat ttactagaga tggaaatgat atttattttg aaatgccaat tacatttgtt
    15361 caagcaactt taggtgatga gatagaagta cctactttgg atggaaaagt taaatatagt
    15421 gtgccagagg ggacacaaac tggaactgta tttagactta aagagaaagg aatccctaga
    15481 attagaggta attcaagagg agaccaatat gttaaggttg tagttgaaat tcctaaaaaa
    15541 ttaaacgata agcaaaaaga actattgaga gaatttgcga aggagtgtgg gtcgaacgtc
    15601 cacgaaaaga agaaaacctt cggacaaaag atagaagata tgtttaagaa aaagtagtta
    15661 aagtagccaa tatataatca tatttttgaa atcttagtta tttataagat tgtcaaagga
    15721 gatattatga tttttatatt ggctattttt gtataaaaaa taaatgtgca taaaataaaa
    15781 ctgaatataa ataattttct gaaaaatact tgtcctaaaa tatataatgt gctataatat
    15841 aatcaaataa aagaaagggg tgattccagt gggaacggat gtggcactgc caaatgatga
    15901 agaaaacttt attgtaatat acaaaataaa attagatact tttagtgcta gattaagtac
    15961 tggaaaaact catgagctgg gataattatt gtaattacct agactcaaac ttttagtagt
    16021 aaaatagtaa tattatatag acaaggtatc taatttttaa atcttttgta tgagttaaaa
    16081 tttttataaa gggtatatct gattagaatg taattaggga aacctagttg tgaattactt
    16141 ttagtatata catgtgtaaa gaaatatgta tatggtattg gttattgtgc tagatgtgta
    16201 tgtaaaggaa tatgtgtcta gttgggtata tgtaaagaaa tatacttata atctatgaaa
    16261 aatttttagt ataaattgct aaaacattga gattgttttt tatagaaagt tagtttatac
    16321 caactaaaaa tgtgcttaag atttgtttga tgtttaatta taaataaggt tgtagataaa
    16381 aaaataagta aagacaaagg tttaaaatac ttgctttaaa tatagtttgt tttgaacact
    16441 tgccaaatta aaataattaa taaaatagac atcttctcat agtaaaatat actgtgagag
    16501 gatgtttttg ctttgttcat aaacttttat gaaaaatttg catagtgtca cattttaata
    16561 tactatatat ataataaaat atagataaaa ttaaatgatt caaaagagaa aggtgaaaag
    16621 agatgtatta tataggtgta gacataggag gaacaggtat acaagcagga gtagttgata
    16681 actatgggaa gataattttt aggtcagagt gtaaaacagt aattgaaaaa ggctttgaag
    16741 gaatacttaa tgacataaaa ataatgattt ataagttgtt ggaagataat aaacttacta
    16801 tgagtgacat aaaatctatt ggatttggtg ttccaggttt tataaataaa gaaggattag
    16861 taacttgtgt aaatttaaaa tggaataaaa aagcttttaa taaagagtta aaaagaagat
    16921 ttccagatgc tgaaattcat ggagaaaatg atgcaacagt agcagcactt ggagaagcaa
    16981 agtttggaag tatgaaaggt gctaatgttg gagtattata tactttagga actggtgtag
    17041 gtggaggaat agtaattaat caaaaagtct tttctggagc acatggattg ggttcagaaa
    17101 taggtcatca aattatagga gaaaactatt ttaattgtaa ttgtggaaat aatggttgtg
    17161 tagaaacttt ttgttcagca actgcaatta taaagtattc tcaaaaactg atagaagaag
    17221 gagaaaagag taggatttta gatttggcag agggaaattt agaaaatgta aatgcaaaga
    17281 tggtgtttga tgcatataga gaaaatgatt tagtggcaat taaagtaata aatagattta
    17341 aagattattt agcaaaaact tttgcaaaca caataaattc tttagaccca gaaattatat
    17401 ctataggagg aggcatttct aaatctagcg atataatact tgatggaata gaagatttgg
    17461 taagaaaatt tgtattatac aaaacagaag atatagctac aattacatgt gcaactttag
    17521 gttcagacgc aggaattata ggagcagctt ttttataatg taaatgaact gcttattgga
    17581 ggcgtgatga tatatgggta aaacaaatgc tagaagaacg ctttttttga tagcaatagg
    17641 ttcaggaact atgctgaatc ctcttaattc ttctatgatt tcacttgctt tacatagtat
    17701 acaaaatgat tttcatatta gttttacaac tgtatcttgg attgtttcag cattttacct
    17761 agctagtgct attgcacaac cagtttcagg taagatagga gatttaatag gtagaaaggt
    17821 cttatttttg agtggtttga tgcttgtaat tttatcagca tccatagtac cacttgttca
    17881 atcattcttt atattgattt ttataagagt aattcaagca attggaacaa gtaccctata
    17941 tccatctgga gttgctctta tacaaaataa tataaaagaa agacaatctt ctgcattagc
    18001 agttctaacc atttttgctt caactacagc aggattaggt ccgacacttg gaggattatt
    18061 gttggactta ggaggatggc atgctatatt tcttgtaaat atacctgttg ttttggtttc
    18121 tatatgctta gggtattttt tattcccaaa agaagtaaaa gaaaagaaat ctataaaaga
    18181 aactttaaaa aatgttttgt caagattaga tattattggc atacttctat tttcaattgc
    18241 aatgattttt atattactat ttttgctatc tatgaaggaa tcttttaact tagaacaact
    18301 tgtatttggt ataatactga tgtgtctttt tatttggcat gaattgagaa caaaattgcc
    18361 atttatagat attaagcttt ttgtaagtaa tcctaaattg tcaaaagttt acttgcaatt
    18421 tattatatta aatttcttta attatatttt gttttttggt cttcctagtt attttcaaga
    18481 tgccttacat tatagtgcaa agtcgactgg attatttatg ctatttatgt ctggtatagg
    18541 tatatttata tctccattaa ctggaaaatg gatagataaa tctggaacta gatttcctct
    18601 tgttacatca tcaatcttta tgtttattag tgcagtactt ctttcagtag tttttatcca
    18661 tatacctgta ataggaaagg gtattatatt atcattagca ggtattagtt atggagttgg
    18721 taatgtagcc ctgcaatcct ctatgtttga agaaagtcct cgtgacagta tagggacagc
    18781 ttctggtctg tttcaaacta gtcgttattt aggttctatt ttagcaactg ttttacttgg
    18841 aatggtattt gaaggaaata taacatcaga acaatttcag atattaggtt atgttatggt
    18901 ggtcttaggt acaataagtt ttttattaaa ttttatatta aagaaagaat taagaaaagt
    18961 agaatagtgg atttatggat ttaataagtc taataactga taagtttaaa aatattgttt
    19021 gtaaaacata actggtttga tatttattaa ttctaagttt tgaagcatag agtactcgat
    19081 aagtatacaa tattttgaat taaaattaag cagaaatttg aactagtctt tcaaatctct
    19141 gcttaattta gtagttaaat ttttttatag agaaaataag atttgacact tagataaaaa
    19201 atcattgcat tttcaaaatt ctttaaatca aacccaagta tctcattaat cttttccaat
    19261 ctatatacta gcgtgtttct atgtaaaaac atactagtag atgtcttaga aatatttaaa
    19321 tcattattta taaattttat tcctgtatat agaagttctt cgtcaataga tttaatctta
    19381 ttaaaatcta tt
//
LOCUS       DACTGW010000054        19345 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.054, whole genome shotgun sequence.
ACCESSION   DACTGW010000054 DACTGW010000000
VERSION     DACTGW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.054"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(299..1498)
                     /gene="coaBC"
                     /locus_tag="I9573_001524"
     CDS             complement(299..1498)
                     /gene="coaBC"
                     /locus_tag="I9573_001524"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAT4796150.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLAFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(1503..1769)
                     /locus_tag="I9573_001525"
     CDS             complement(1503..1769)
                     /locus_tag="I9573_001525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAT4796151.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(1772..2389)
                     /gene="gmk"
                     /locus_tag="I9573_001526"
     CDS             complement(1772..2389)
                     /gene="gmk"
                     /locus_tag="I9573_001526"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAT4796152.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(2419..3300)
                     /locus_tag="I9573_001527"
     CDS             complement(2419..3300)
                     /locus_tag="I9573_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAT4796153.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(3304..4134)
                     /locus_tag="I9573_001528"
     CDS             complement(3304..4134)
                     /locus_tag="I9573_001528"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAT4796154.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            complement(4421..5455)
                     /gene="ltaE"
                     /locus_tag="I9573_001529"
     CDS             complement(4421..5455)
                     /gene="ltaE"
                     /locus_tag="I9573_001529"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAT4796155.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            5751..7526
                     /locus_tag="I9573_001530"
     CDS             5751..7526
                     /locus_tag="I9573_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAT4796156.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEIANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIVLSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(7741..9393)
                     /locus_tag="I9573_001531"
     CDS             complement(7741..9393)
                     /locus_tag="I9573_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAT4796157.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEYLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGLLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLLEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGCFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRDKQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQITKLKNSF"
     gene            complement(9692..10903)
                     /locus_tag="I9573_001532"
     CDS             complement(9692..10903)
                     /locus_tag="I9573_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAT4796158.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLLLRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(11187..11720)
                     /gene="pyrR"
                     /locus_tag="I9573_001533"
     CDS             complement(11187..11720)
                     /gene="pyrR"
                     /locus_tag="I9573_001533"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAT4796159.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(12079..13026)
                     /locus_tag="I9573_001534"
     CDS             complement(12079..13026)
                     /locus_tag="I9573_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAT4796160.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKAKYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDFVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEAIKKIK"
     gene            complement(12995..13441)
                     /gene="lspA"
                     /locus_tag="I9573_001535"
     CDS             complement(12995..13441)
                     /gene="lspA"
                     /locus_tag="I9573_001535"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAT4796161.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(13751..14530)
                     /locus_tag="I9573_001536"
     CDS             complement(13751..14530)
                     /locus_tag="I9573_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAT4796162.1"
                     /translation="MKKAIWGICICLLSIVGVYTYNHNTHNQNSSKETYTLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQILDILKKNNVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYGSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDTIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(14580..15062)
                     /locus_tag="I9573_001537"
     CDS             complement(14580..15062)
                     /locus_tag="I9573_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT4796163.1"
                     /translation="MDTKKYKDKLLKEREKLTNLVVDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDSALSKIENGTYGFCDLCHNQI
                     DLERLDILPDTNLCNNCAKKENALLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGTLRD
                     "
     gene            complement(15246..16667)
                     /locus_tag="I9573_001538"
     CDS             complement(15246..16667)
                     /locus_tag="I9573_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HAT4796164.1"
                     /translation="MVSFLGSIVVLIVGYFIYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGIVVAI
                     ILGLLFFKKAKDIKEQNAHKLAS"
     gene            complement(16813..17583)
                     /locus_tag="I9573_001539"
     CDS             complement(16813..17583)
                     /locus_tag="I9573_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4796165.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSSIEVVNEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYENYAVDAFSLDVFDYVLKP
                     YSEERIISMLNKLEKSEMSDIELSNSNSNLYKYKKETVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(17588..18790)
                     /locus_tag="I9573_001540"
     CDS             complement(17588..18790)
                     /locus_tag="I9573_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT4796166.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIVGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRTDNGT
                     LIVFYVKE"
BASE COUNT         6376 a         3419 c         1981 g         7569 t
ORIGIN      
        1 tattaccttt gcatattttt tcattataaa acctctactt aaatttaata tattgttcat
       61 attttaatat actttacaag atattaaaat ctcagaattt ataagcaatt tcaagttatt
      121 aatattaact ttattttaat aattaactat taattatatt ttatacttta tgacaaaata
      181 aatgctgaaa actacaattt aactgacatt tgcgagtttt tactcgctaa aattaaattg
      241 cagctttcag ccactttagc tgtgataaaa tacacaaata tcatatttaa aaaaggtctt
      301 atttctcaag caacgcttta actttatcta aaataacatt ggctacttct tcttttttca
      361 tcttaggata ctcagtaata tttccctcta catctattat ttttacaata ttagtatcag
      421 ctctaaaacc agcaccttct ttagttaaat catttgccac aataaaatct aaatttttct
      481 tttttatttt tcctttggca tgttctataa ggtcattagt ttcagctgca aatccaatta
      541 gtatcttatc ttcttttatt tttcctattt cataagcaat atcataattt ctacttagtt
      601 ctattgataa atcatcattc ttctttttaa tttttttgtc agaataagtt tttggtttat
      661 aatctgcaac tgctgcactt tgaattacta ttttattttc acttaaattt gatactactg
      721 cttcatacaa atcttgtgct gactctattt gaacaaattt tttaagattt tgtggaatag
      781 aaagatttgt aggacctgat attaatgtta catcagcacc tctagctata gcttctcttg
      841 ctatagaata tcccatttta ccagaagacc tattagttaa atatcttact gggtctatac
      901 tctctacagt tggaccagct gtaactatca tactttgacc ttttaaatct tgctctttag
      961 taagattagt caaaacttcc tctactatta tgtctggatt agctaatttg cctgttccaa
     1021 tatctccaca tgcaagcctc ccactttctg gttcaataaa tttatatcca aagctcttta
     1081 aagtttgtat atttttttga acaattggat tttcatacat attagtattc atagctggtg
     1141 ctataagaac ctgtgctttg gttgccataa cagtagttgt caacatatcg tcagctatac
     1201 catttgcaat cttgcctata acatttgcac ttgctggaac tattaagaat aaatctgctt
     1261 tttttgctaa tgatatatgt tcaacttccc aagtttttgg ttcttcaaac atatcttcta
     1321 ctacataatt ttgactcagc gattgaaatg caagtggttt aacaaattct gttgctgatt
     1381 ttgtcattat aacatggata tttgcattta gtttttttaa cttacttaca acatcacaag
     1441 ccttatatgc agctataccg ccacttatgc caattacaac agttttatct tttagcatga
     1501 ccttactcct cttctgaaat ttgttggtgt tgctcagcat gatgtctagc ttcttctatc
     1561 tctatttcct cttctgtcaa taatctatat gttatttttc cacttgcaac ttctttagtt
     1621 gctacacata caggcttttc cttagtttta tttgaaacat atggttcttc tccatcaatt
     1681 aattttcttg ctctctttga aacagtccct actaaatagt atctgttgtc tattttttct
     1741 agtacctcat tgatagatgg ttttaacata attataattc ctccttaaat ttctctatta
     1801 taatattttt atatctgcta actttattct tttcagatga tattatacct tctatttctt
     1861 tagcagatgt ttttacatct tcattaaata taaaataatc ataatcctta atcattttaa
     1921 tttcttcata tgcacagcta aatctcttct ctatttcagc ttctgtttct gtacctcttc
     1981 caacaattct attttttaat tcttcaagtg atggtggtag tacaaatata aatactccct
     2041 ctgggcaaac ttcttttatt tgttttgctc cttgcatttc aatctccagt aatacatctt
     2101 gtcctttttc taaacattcc attattgccg cttttggagt tccataaaaa ttatcataaa
     2161 tttgagcata ctccaaaaac tcaccttttt ctatcatctc ttcaaacttt tccttactta
     2221 tgaagaaata atttactcca tcaacttctc cagttctggg ttttcttgta gtagctgata
     2281 cagaaagttt tattgtatca ttttctttaa gtagttcttt gcaaatagta ccttttcctg
     2341 cacctgatgg tcctgataca actagcaata atcctttttt cacaaacatt taagcattcc
     2401 ccttctaata ttgtattctt actctatatt ttgtatttgt tctcttattt tttccaattc
     2461 gctttttact tctactacta aattagttat atttatatca gatgacttcg aacctatagt
     2521 gttagtttct ctattcatct cttgtattaa gaaatctatt tttctaccta tagagtcgtc
     2581 tttaaatata gtatttttaa gttgccctat atgactttta aatctaacta tttcttctgt
     2641 tatactgctt ttatctgcat atattgcaac ttcttgtgct aatctattct catctattat
     2701 acttgggtca tccagtaatt cacttattct caaattcaat ttttctctat aatcttctac
     2761 aacactactt gaatattttt caatctcttc tatatgattt tttaataaat cacatctcat
     2821 ggcaatatct tcagctaatt ttttaccttc ttcacttctc atttctctta atttgattaa
     2881 tgcatcttca actgcttgat tcaacattga ccacaataaa tcttcatctt cttccttttc
     2941 ttctattttc acaatatcag gaaactttgc aacattcata acgctaatat catctataat
     3001 atcaaattta ttttttattt cttttagtat gtctatatac tgagatgcta atccctcatc
     3061 aaaatttaag tttacatctt ctttgcctaa taagtcaaac tttatgtata aatctactct
     3121 tcctcttttt acataatttt ttactaaatt tctaacttta tcctctaaaa atgaaatttt
     3181 tcttggaaga cgtatgttta tatctgaata cttatgattt atcgttttac actctactaa
     3241 aaagtaataa ttatcatcct tgtattctcc tcttccaaaa ccagtcatac ttatagccat
     3301 taattaaacc tccaaaacac cttcacatat ttttactgct ggacctgtca tataagagaa
     3361 atctcctaca tctattttaa ctgttccacc ttgagacgtt acatttacac tactttctac
     3421 catattaagt aaattgcaca ctattgctga tgcagtcata ccagtaccac aacctaaagt
     3481 atatccacaa cctctttccc aagtataaac atgtatatta tacttatctt ctacctttac
     3541 aaaatttaca ttcgtttttt caggaaatat tttattattt tcaatctctt ttccatattt
     3601 acaaacatca tctacatcta attcatctac tataactact gtatgaggaa cccccatcaa
     3661 catagaagta actttaagct ccctatctaa aacctttata ctttcttgca caaaacgttc
     3721 tttatttgta agtacaggaa catccttagc tataaaactt ccttttccca tattgactct
     3781 tatagagttt atttctctat tggtaagatt tatttttata tcttttatac catctaatgt
     3841 atctactgta aaactttctt ttttaactat acaattatca taaacaaatt tacaaaaaca
     3901 tctaaggcca tttccacaca tagctgctct tgaaccatca gaattataat ataccatttc
     3961 tacatctgca acttttgaat ctttcacaac taacaatcca tccgctccta cagaaaatct
     4021 tctatggcat actttttttg ctaaagcagt ataatcagat ggatttatct ggtcgaatct
     4081 tccatctatt gctataaaat catttcctat tccatgtagt ttccaaaatt tcataagtat
     4141 cgctccttcg taaattaacg cttaaactat tataacataa aaaaattccc ataaacttat
     4201 aatttatggg aaaagttttg taatgacaac tataatttga ggatgtgagg gagagttgtc
     4261 attacaatta gggtaattat attatgtttt taagctagtt ctttctatgt ttatatatta
     4321 atatttctat atgaatttta tgtatgtttt atttaaactg tctatgaatt ttttaattaa
     4381 acatctggta accagctata atccttttag tagatatcta ttaacataca cctattaatt
     4441 ctttaagtgt ttttaaaaca tagtctaaat cttccttagt aatatctaca tgagttacaa
     4501 atctatattc accattttcc attttattaa tttttatatt tctttcaaaa aattctttta
     4561 ttaaaacatc ctcttttata atatcttcac tcatcttaaa aaataccata cttatatctg
     4621 gattaacatt gtttacttca atgccatcaa ttttatttaa ttcacaagct aaatattttg
     4681 cattttcatg gtcttcattc aatcttaaag tcattttttc aatggcaatt ataccagctg
     4741 ctgcaactat acctacttgt ctcattcccc cacccataac tttttgcttt cttctagctt
     4801 ttgctataaa ttctttactt ccagcaagta tagaacccat tggtgcagct aaaccttttg
     4861 acacacagaa cataacacta tcacaacact ttgctatttc tttagcttct acacccaaag
     4921 ttaatgctgc gttaaataat cttgctccat ctaaatgtac tggtaagttg tgtttctcag
     4981 ctattttttt aatttctctt aaattatcaa gactcacaac agttccacct gaatatgcat
     5041 tttctacaca tataagccct gtctgtggtt catgtatgtc atctcttcta attgcctttt
     5101 caacaagttc aggatttaga gctccacttt cgtcttcatc tatagttcta agctgaactc
     5161 cagctattac agcaggtgcc ccaacctcat gagttacaat gtgataacct ttaccaataa
     5221 ttatttcttg ccctcttagg caatgagtaa atagagctaa ttgattccca aaagttccac
     5281 ttggaaggaa aatagcatct tcttttccaa ctttagctgc tgctaattct tcaagtttat
     5341 ttacagtagg gtcatctcta aagattgaat ctccaacagg agcctttgcc atagcttctc
     5401 tcatttcgtc agtaggcata gtaacagtat cacttcttaa atcgataaat ctcatttttt
     5461 caaccttctt tcatatttat atactataga accttaaaat tgattttttc tgtaaagttc
     5521 atatactttc atagcttata ctctacacaa taattatact atatttactt tatatttaat
     5581 ctactttaaa ttattaattt taaaaataaa gtttcctctc taataagtat aaataattat
     5641 acaatttaaa tattaatctc tatcgtttaa attcccccta gtcacaaagc gtatattatg
     5701 tgtataatta atttactgac tcattttggt caaagaaagg ggtgctataa atggcattag
     5761 atggattagt tatacattca atagtcgatg aattatcatc aaaacttaca ggtggaaaaa
     5821 tagataagat acatcaacct gaagatgatg aggtaatttt taatataaga aataataaag
     5881 aaaattttag attggtttta agtgcaagtg cctcaaatcc tagagtttat cttacaagta
     5941 attatcaaaa agaaaaccct ctaaaagcgc ctatgttttg tatgttactt agaaaataca
     6001 tacaaggtgg aaatatagta gaaatttctc aaataggttt tgaaagaata ataaaaataa
     6061 gtgttgaatc tttagatgaa ttaaaagaaa agacagttaa aaatattatg attgaaatta
     6121 tgggaagaca tagcaacata ataataactc atggtgaaga aaataaaatt atagattcta
     6181 taaaaagagt cccttttagc ataagtagag ttcgtcaagt tttacctggt catgattatt
     6241 cattaccacc agaacaaaac aaactcaacc ctcttgatga tatatctaag gatttgttta
     6301 ttaaaaacct agaagaattg gaaggaccta tctttaagtc tatatattct aaatttctag
     6361 gtataagccc tattatagct aaagaaattt gctacagagc aggtgtaaat caaaatgcta
     6421 tcataaagga tatatctgat gaacaatttg attctttaca caaagtattt tgtaatttat
     6481 tcaatgatat aaattctaac aaatactccc cttgcattat tatagataaa aaggtggata
     6541 aggtagttga tttcagttgt ataaacctta cattatttag cgatttatca tatataaata
     6601 aggatagtat gtctcgtatt ctagaagatt tctatagaac taaagatatt aaagacagaa
     6661 ttaatcaacg ttcatctgat ttaaagaaga gtatatctgt taagcttgat agactttata
     6721 ataaacttaa aaaacaagaa gaagaattat cagaatccga aaatgcagac atttataaga
     6781 taaaaggaga attgattact tcatatattt atatggtaga aaaaggtatg gaaagtattg
     6841 aaattgctaa cttctatgat gaaaattgta atgatgtaac aatagaatta aataaaaact
     6901 tgacaccatc tgaaaatgct caaaaatact ttaaaaagta taacaaaatg aaacatgcta
     6961 aagtcgagat atctcatcaa atttccttaa ataaggaaga aatagattac ttagaaaaca
     7021 tagttttaag tattgaaaac tgcgaaaatt tagctgaatt acaagatata aaagaagaac
     7081 ttgctaaggt tggatatatc aagactcaaa agaaaaacag taaaaaggat actatcccat
     7141 ctacaaaacc tcatgaattt gtgtcttctg atggatttaa aatattggtt ggtaaaaaca
     7201 ataagcaaaa tgactatctt acactaagac ttgctgataa tgatgactta tggatgcata
     7261 caaagaatat tcctggttct catgtaataa taaagtgtgc tggtaaggaa gtccctgata
     7321 atactgtatt tgaaggagct atgctagcag cattctttag taagtcaaaa ctttcttctc
     7381 aggtgcctgt agactatact aagagaaaaa atgtgaaaaa acctagtggt tctaaaccag
     7441 gtatggttat ttatgaaact aacagtacta tttatgttac tcctgaagaa gaaacagtag
     7501 ccaagcttaa ggttaaatct gaataaattc tgcactaaat acaaaataaa tgttagacaa
     7561 gcacacatta tcattctaaa taaaaatagt ggccttctta taaagtacaa taataaggcc
     7621 actttctaat tagcttactt ttaattttct aacaaaatac ttttctgttt taataactat
     7681 acttatattc ccaacaaata ttttcagtct tcttatatag atgtaattaa aaagttaaca
     7741 ctaaaagcta ttttttaatt tagtgatttg ttttagtaat tcattatatt ctgattcata
     7801 caattctttt tttagattat ctttttctat agataacata gatataacag ttgtaagtct
     7861 attttctaat actagaagat tctctttttt gatttgcttg tctctactag gagttttttt
     7921 atttctgcta attatatatt cgtcataatt acaatcaaac tcccttattt tagcatcttt
     7981 tatctctata atatagttac aaatatgtga tacaaatact ctatcatgag acactataag
     8041 catagtttta tcagtattta tcagtgcctt ttccagtgct tccatagatt ttatatctaa
     8101 gtaatttgtt ggttcatcaa atactaaaaa attattatct tccaatatta ttttacatag
     8161 tgctattttt accttttctc caccactaag tacttttact tttttgtaga catcatcacc
     8221 tttaaaccca aataggctta agtttatcct tataaaagat tcatcaaatg aactattaac
     8281 tttagtattg tatagaacac tcttttcctc atctaataaa ctttgatttt ggtcaaaaca
     8341 acctactttt accttgttat ttatttttat attgtcattt ttatcagcca atatttcttt
     8401 tatcagagta ctttttccac acccattatc acctaataat gctatctttt tacctctttt
     8461 tatcttaaag gaaacattat ctaacaagag cttattttct aactttagag tcatatcttt
     8521 tacctctaat agatttttgc taattatctc cataccatct tgaatcttaa tgttcatttc
     8581 tttagaaact ttaggtttat tctttacctc aagtttatca attctgcttt ttatagcctt
     8641 cacatttgag tccaactttt tcttattagt ctgaccaccc attttgtgca acctagcttc
     8701 tgaattaccc attctttttg gtgtctttct tatgccatca cttaaagctt ttttatataa
     8761 aagagctttt tcaagccttt ttttctcttt aacatattct tgatactcaa aatcagctct
     8821 ttttacttct tcatcttttt gcattaaata atcagtgtaa ttgcctttgt aagcttttag
     8881 ttttccatct tctagttcta atatagttgt acataactca tccagaaaac gtctatcatg
     8941 agatattaat aataatgctc cctcatgcct tttaaacata tcttccaaaa gcccaatgct
     9001 ttttttatct aagtttgaag ttggctcatc agctattatg atatttttct tattactcag
     9061 cgcatctgct atttttaact taaccttttc ccctcctgat aagtattctt caaatttatc
     9121 tggagcattt aaaagactct tctccttact atgactacat gtttcaatat tattttcact
     9181 ctgacttata taagaatagc tttctgtaag gtaagtatat ccttcatcaa tttctatttc
     9241 acccaatatt gcttttaata atgttgtttt tccttgacca tttgaaccaa ctagacctat
     9301 tttgtcattt tctaatattt caagtttatc tatatccaaa attaatttat ctgcataata
     9361 tttctttaaa ttctctactt ttactaacaa cataaaaaat ccctccttaa cacacttaaa
     9421 tataaggaga gattctataa ataggcataa caaataaacg tatagactat aatctactct
     9481 ccttaattta agcattgcaa ataaagccat caaaataggc ttttcaaaaa ttacaatact
     9541 ttaaattata aaaaatagat tatattctca ttgtcctagt taccttgccc tttctttcat
     9601 ttatttttaa tcattatatc aacattaatt acaaatgtct atagaaaatt aactaatttt
     9661 agttattcaa tatacaattt attctttaaa tttattcttc ttcaacttta aaatcctgat
     9721 tatttaaaat tctgtttaat acaattccaa caattgcagc taaactaaga ccagttatct
     9781 ttactgtact tgttataggg attccaatag aaatgccttt attagataaa tatgttgttc
     9841 caagacctat taataatata actgctatta ttaatatatt tatcttactt tctacacaag
     9901 aactgtccct tatagttttt aatcctacat aagttatcat tgaaaatagc attatactaa
     9961 ttccacccat aactgaacct ggtatacttt gaagaaatcc accaaacttt cctacacatg
    10021 caagcaatat agcaaatatt gctgtacgtc taagtattga agggtcatag tttttagtta
    10081 tagcaagtac tgctgtattt tctccataag ttgtattggc tggtcctcct aaacaccctg
    10141 caactatagt cgcaaatcca tccccaagta atgttctatt taatcctgga ttttctataa
    10201 agtttttacc tactacagca ccattagtag tcatatctcc tatatgttcc ataaatacag
    10261 ctaatacaac tggagatata atcacaattg ctcctaaact gaacttagga actgtaaagt
    10321 tcggtattgc aaataaactt gcttctgtaa tagtagttat atctactaac cttaacagta
    10381 aacagattat atacccagaa aaaacggcta ttaatattcc taattgtttt ataaatcctc
    10441 taccaaactt attggttaaa attgctattg ctaaagttat aaatgctatt acaaaattag
    10501 ctgaagccat actaaaagct gttggcaata aatttaatcc tataactact atcatagctc
    10561 ctgtaacttg tgcaggaaaa tataatttaa ttttatttac accaactatt tttactataa
    10621 tcgacattat aatataaatt aatcctgcta ctattatacc accttgagca tatgctaaat
    10681 ctccaccata cgcctcttta gctgctagta taactggtat aaacgcaaaa cttgaaccta
    10741 aaaaggcagg tacttttcct tccgtacaaa aatgaaatat caatgtacca acaccagcac
    10801 agaaaattgc aactgacgga ttaaaacctg tcaaaatggg aactaatact gtcgctccaa
    10861 acatggctaa tagatgttgt aaagacatta ttgttttttt cataaaaacc tccaaaatta
    10921 ttatatataa ataaatcctc tgtttaatta atatctttta attaattatc attccctaga
    10981 ttctattttt taacctctat atacttatta tataatttat cattatttta cattttacga
    11041 catcaaaaac tcaaataaag gagaataaaa actacattaa gactcacatc cataaaatta
    11101 taatatttat tttgtgcaat ctatgtaagc tttatcctcc tccctgacta ttcaatgcta
    11161 agcttttata taatacttaa aactaattat tctttgattg taactgaatc ttctccatca
    11221 atttctaata aactgacaga tataatttca tgtctagaag taggtacatt ttttcctaca
    11281 taatctgctc ttataggcaa ctctctatgc cctctgtcca ctagaactgc taattgtatt
    11341 gatttaggtc ttcctatatc tataatcgca tctagtgaag ctctcacagt tcttccagtg
    11401 tataaaacat catcaaccaa aactactgtc ttatcattta cattaaaatc taaataagtt
    11461 cctttaacta caggttcaac atgaattttt tccaaatcat ctctatataa agttatatca
    11521 atatctcctg tatctacttt tgttccttct atttgttcta ttttcttaga aattctattt
    11581 gcaattggca cacctctagt ttttattcca actaaaacta gattttctac acctttattt
    11641 ctttcaatta tttcatgact tattctagta attgctcttg ctattgcctt ttcatccatt
    11701 aattgagctt tttctaccat atcataatta ttgtcaaaga tataatatat ctatttgaca
    11761 aatctactcc tttcttttaa ttttttataa atttattttt atttaaaatt tcaatttaaa
    11821 caacaaaaaa cttctcatca caagacaaga agctcttata taaaaaatat acagtgtaat
    11881 ttagcaattg tcttacgata tctctgtatc gtttaaagat aattttttaa tctatcaact
    11941 caaattatat atttatattt caaatttttc aagctttttt aaacaaaata ctttaatata
    12001 agatatttta tttaaatttc actaaattta tttacttata taaaaacaaa aaatgattta
    12061 taagtattat acaaatggtt attttatctt cttaatagct tccttaaaat actcaggtaa
    12121 ttctgaatta aattcgatat attccttagt ggtaggatgt ataaatccca acttcttagc
    12181 gtgcaaagtt tgtcctttta atttaaattt agtttctttt ggaccatata caaaatcacc
    12241 taaaagtggg tgatttatag acaaagcatg aactcttatt tgatgagttc taccagtttc
    12301 aagcctagct ctcatatgtg taaacttatc aaatctttct acaacttcaa aatgagtaat
    12361 tgcatttttt ccatccttta ctacagccat ttttaatcta tccttaggat ttctccctat
    12421 aggtttatca acagttattt tatcttcttt aaccacacca tagcatataa attcatactc
    12481 tctagttata gtatgttcct ttaactgttc tgctagaaaa ttatgtgcat aattgttctt
    12541 agctatcata agaagaccag aagtatcttt gtctattcta tgaactatac caggtctaat
    12601 aactccattt attgacgaca acttatcttt acaatgatat aaaatcccat ttactagtgt
    12661 attttcatag tttccaggag cgggatgaac aaccatatcc tggggtttat ttattattaa
    12721 gacatcatca tcttcatata caatatctat tggaatatct tgaggtaaaa cctctaatac
    12781 tttaggttct ggtatctcta taacaatatt atcttcctct ttaactagat atttagcttt
    12841 ttctacttta ttattgacct ttacttttcc atcttttata attttttgta tataacttct
    12901 tgacatatca cctaattgtt ctgctagata aatatcaagt ctaacttctt cttcctcttc
    12961 aagtactaaa aactctttta cttcatccat tatatcacct acttttactt tcaaaaaata
    13021 aaacatatat acataaaaac acagttccta caactacaaa tacatcagct acattgaata
    13081 catattccca tataactcta aagtcgaagt aatctaccac aaaacctaat cgtactctat
    13141 caattagatt tcctaatgca ccagatataa ttaatataat tcccaacctt ccaaatatat
    13201 gtactttcct tgtattaaga tagtataatc caaatactgt tgcaagtaat gcaacaatta
    13261 taaatatcca ctgattattt tgtaataatc caaatgctgc acctctattt tcgacataag
    13321 ttaaatgaaa tacattattt attattggta ttgttgaaac atctaccaaa ttattcaata
    13381 cccatatttt agacagttgg tctaagccta tgagtagaat tattattaat atatatagca
    13441 atgatttttc cccctataaa ttatttaaaa accaatttta tttgaaacac actattatta
    13501 ttatacaaaa aaaatgctct aaagagcaac tttattaaaa ttgtattttc ttaataaagc
    13561 tagagcaaaa tttaaaatag taccatcttt tttatattgc agattgctac atatttaaaa
    13621 tcatattatt atgtataaaa aatttaaaaa taacatacta attaatactt tttgtgtgtt
    13681 ttatagatta aagattaatg atgtctgtac tttaaactta caagcaccac taatcttcaa
    13741 acaaatattt ttacttttgc acttgcttat ttttatctaa atgttgtata agttcagata
    13801 ctgttacgaa tttatatcct ctctctttta atttaggaat tactacttct aaagcttgta
    13861 tagtttgtga tttatcattt cttttagtgt tataatcatg taaaagtatt atattaccat
    13921 tttggacctt tgtaagaata gtatttataa ttgtatctac accagggtta gaccaatctc
    13981 tagggtctaa gtttgaccaa agaattatat tcatgccttt agaaactata atatcacaga
    14041 cacttttact catggctcta tatggaggtc taaatatctt tggttctact cctattacac
    14101 tttttataat attttgtgta tctgttattt ctttttcaac actaccataa ctattttttg
    14161 caacatttat atgtgtaaat gtatgattcc ctatttcatg cccctcatca tattgccttt
    14221 ttaataattc tttgttatat tctacttttt ctccaactac aaaaaaagtt gcttttacat
    14281 tatttttctt taatatatca agtatttgtg gtgtaaaatc ctcatctgga ccatcatcaa
    14341 aagtcaatgc tagaatcttt tcatcctcat ttcctttttt tattattaca tcttcataat
    14401 cgtcttttat attgtcattt aaagtatagg tttcttttga actattttga ttatgtgtat
    14461 tatggttata tgtatataca cctactatgg ataataaaca tatacatata ccccatattg
    14521 ctttcttcat gtcattacct cttttctaat tcattctaac aatatatgat gtcaggcttc
    14581 taatctctca atgttccatt tttatttaaa tcagttaaat ttgttaggtc ttctgaataa
    14641 aagttagaat cttctacatc aggattttgc attgcatcac ctaataaagc attttctttt
    14701 tttgcacaat tgttacataa atttgtatca ggtaatatat ctaatctttc taaatctatt
    14761 tgattgtgac ataaatcaca aaacccatat gttccatttt ctattttaga taatgctgaa
    14821 tctatttgat acagtcttcc ttcttcatga tttattaaac tattttgcat attttgcata
    14881 tataaatccg ttcccatatc tccaatatga ttgtcataac tagataattc tcctgatgaa
    14941 tatttttcac tagtatgttt agtagtgtct ccaaacaagg tattatcttt catatctacg
    15001 actaaattag ttaacttttc tctttctttt aataatttat ctttgtattt tttagtatcc
    15061 attttcaaca cctctaattt taatatttat atcataaaaa tagtatgtgg aaaactaaat
    15121 taaaaataca ataaaaaaca agatttgatt ttttcaaaaa aaattgtagg taaacttaaa
    15181 attcacctac aaattcttta catatatttt taatataatg tttttagttt ttgataataa
    15241 ttacattaac ttgctaattt atgagcattt tgctctttta tatccttagc ttttttaaaa
    15301 aataaaagtc caaggattat tgcaactaca atacctattc cattagatat attaactgga
    15361 agtctaaatc cttcttttgc ctgcattata taactaaatg ttactacagt cataaaaagt
    15421 gctggcaatg tagttatgta atgattttta tttgctttga ctaaatatgc agaaccagtc
    15481 cacaacatta tcatagcaag tgtttgattt gcccaagaga aatatctcca aattatatta
    15541 aaatctataa atgtaagggc aacccctaca gcaaataatg gcaaagctat taaaaatcta
    15601 tttttaactg catcttgatt ataattcatt gcatcagcta ttgttaatct agcacttcta
    15661 aatgctgtat cccctgaagt tataggacat gctacaacac caagtatggc caaagcgcct
    15721 ccaataggtc ccattaatcc aacagatact gtatttacaa cagttgctgc tccaccatca
    15781 ttaaatatta cattaagctg aggtatacca tggaaatatg acatagctgc tgctgcccaa
    15841 actaaagcta ttataccttc tgcaaccatt gacccataaa atactggtct tgcctcactc
    15901 tctttctcaa cacatctcgc cataagtggt gattgagttg catggaaacc tgatattgct
    15961 ccacatgcta ttgttataaa taagtatggg aataaaggtg ttccatttgg attcatattt
    16021 tgtaaattta tatttggtat gttatacccc tttattatta atcctccagc tacacctact
    16081 gccataatta ataatgctgc tccaaataaa ggatatattt taccaatcaa tttatctatt
    16141 ggtaaaacag tagccataat ataataaagt attatcactc caacccatat ttgtgcgttt
    16201 acacctgtca atgtctttaa taatcctgct ggacctgtta caaatacaac tccaaccaat
    16261 actaaaagaa taacactaaa tattctcatt gctatttttg caccatttcc tagataaaga
    16321 cctaccaact ctgatgcaga tgtacctttg ttcttcattg ataaatatcc tgaagcaaaa
    16381 tcgtgtactc cacctgcaaa gattgttcca aacacaatcc acaaaaatgc tgatggtcca
    16441 aataacgccc cttgtatagc tccaaatatc ggacctaacc ctgctatatt tagaaactga
    16501 attaaaaaga ttctcttcca tttcattggc acataatcta ctccatccct acaagctata
    16561 gctggagttt ggtttttgtc attgattcca aaaacctttt ctacaaaagt accatagata
    16621 aaataaccta ctataagtac tactattgaa cctaaaaagc ttaccataaa ttcatcccct
    16681 tagtttttat ataattgctt tataaatttt tatgagttta agaaaactat ttccagttca
    16741 aatagtaata cttttttatg ctaatataaa tattaattac attaagtgca tatttaagta
    16801 catgaaatga ctttatatat gcattaacag cctaaattct tttactttgc ttctgcttac
    16861 agttacttca tagtcactat tttttaattt caatatgtat gtattgttaa accatggtat
    16921 aatttctttt actttggtta aatttacgat ataagaccta tgtgttctaa aaaatgtttt
    16981 atcatttata agattctcta cttctgatat gccttctttt aatataaact tttctttctc
    17041 agtatatata aatgtctgcc tttcattagc ttcacaataa tatatatcat caatatctat
    17101 tacaaccaat ttatcacctt tccaaaggct aattttatgt gttatttctt ctatctcctg
    17161 atttactgtc tcttttttat atttatataa attactattg ctattagaca gttcaatatc
    17221 actcatctca cttttttcta atttattcaa catagaaata attctctctt cagaatatgg
    17281 cttcagtaca taatcaaata catctaaaga aaaagcatct actgcataat tttcatacgc
    17341 tgttataaaa acaattttag gccttgattt aaactgattt aatgtttttg caagaagcat
    17401 tccatcgaga tgaggtatat ttatatccaa aaaaattaca tctattttat tttcctgaat
    17461 aaagtctaac acttctatac cattagtaaa ttcatttact acctctatgc tacttttatt
    17521 ctcaataaaa tatcttaatt cctttcttgc cggaaattca tcttccacta ttatagctct
    17581 catacttcta ctcctttaca taaaatacta tcaaagtccc attgtctgtt cttcttatat
    17641 taagccctct tccatacatc agtttaagcc ttaaatgtac attataaagt ccaatcttat
    17701 tttctggcat attatcttga tatacattat ttataatttc ctcacttata cctataccat
    17761 tatcctctat tgatattctc acagtatttt ctaatgaata aggtaacttt tttatggata
    17821 ttttcactac accagctcta ccactttcta aaataccatg tattatcgca ttttccacta
    17881 atggttgtat aatcaaactt ggaatcttta tatttacatc ttctatatca taagatacag
    17941 ttagtaattc tccaaaccta gctttttcta tctctacaaa agatttaact tgttcaatct
    18001 ctttatttat atcaattaag ttatcactat attcaagatt atatcttaga taagttgata
    18061 aatttattat taactctcta gccttatctg gactaaatct tataaatgaa gctattgtgt
    18121 ttagggaatt aaacaaaaaa tgcggattaa tttgtctttg aagtgctttt atttctgctt
    18181 tatttctcgc ttctatatct tttttatctt tctcaatccc ctctactata gatataacaa
    18241 atcctattcc aatctgtcca acaattaaag gtaaatatat cccaacaatt atttgaattg
    18301 ctaaagaatg tggatatgat attaagtata taagtagtat agtaaaactt tcagaaatca
    18361 tacctaccaa tataccatac attactctat gttgctttgg tatcctctta tatagaaatc
    18421 ctgataatac acctgctagt atacttgata aaagacatgg aacagatgtt atccccccaa
    18481 tatccataaa atatctatga atccctgaaa aaactcctgc tgtaatacct accattggac
    18541 caaataaaat gccacctgat actatagata ttattctagt gtttactatt gaccccatat
    18601 aatttatacc actataagtt ccaaatatgg ctaaaaaagt aaaaactaat gctatacaga
    18661 ccaagtcatt aaaactatat tcttcctttt gaaatatctg cttaaatatt tttaactttg
    18721 ttattagaag aaacagtact acaatgaggc ttgtcttttc tattaatgcg attaaaattg
    18781 atgttttcat attccacctt taaaaaataa atttttacac actctaaatt tattatgttt
    18841 gttgtaactt atatatttga agtaatccta tctcgacatt ttccacattt aaccttttaa
    18901 ttaaataaaa aatggatata cttctattaa caactaatac tagctgacta acacaagctt
    18961 gtaataactt gtagttatca ttaactctac aattctagta tttaatttta tactttagaa
    19021 atctcttttc ttagaagtaa tactttcgaa tattatataa tacaatgtta tgtagattct
    19081 tactcataca acatatttct ttaaaaattt tatactattc tttgttcaaa tatctcattt
    19141 gatttaattg tgctaaacac acattatcat ctctctgtta tatatttatt atatagccgt
    19201 atatcgataa agtaaataaa tcctatattt aaaatcatat gtttgatagt aaatcattat
    19261 aatgtatatt taccataata atttgccaac cttgaaagtc acttctataa ttagaagtta
    19321 aataattctt ctaaaaaaaa tacct
//
LOCUS       DACTGW010000055        19323 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.055, whole genome shotgun sequence.
ACCESSION   DACTGW010000055 DACTGW010000000
VERSION     DACTGW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.055"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..854
                     /locus_tag="I9573_001541"
     CDS             <1..854
                     /locus_tag="I9573_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAT4796167.1"
                     /translation="FLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDK
                     AQELIDKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSY
                     ETLRDNVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINK
                     IGSAGKVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGA
                     VTKTITKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKA
                     FKGELFN"
     gene            887..1195
                     /locus_tag="I9573_001542"
     CDS             887..1195
                     /locus_tag="I9573_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796168.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            1228..1608
                     /locus_tag="I9573_001543"
     CDS             1228..1608
                     /locus_tag="I9573_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAT4796169.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            1598..2026
                     /locus_tag="I9573_001544"
     CDS             1598..2026
                     /locus_tag="I9573_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796170.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            2076..5411
                     /locus_tag="I9573_001545"
     CDS             2076..5411
                     /locus_tag="I9573_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAT4796171.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEKLRKSLTEKRKVNTVNYDFKIDEVNEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGKYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMVTAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            5792..7855
                     /locus_tag="I9573_001546"
     CDS             5792..7855
                     /locus_tag="I9573_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAT4796172.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFND
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDVYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(8072..8638)
                     /locus_tag="I9573_001547"
     CDS             complement(8072..8638)
                     /locus_tag="I9573_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAT4796173.1"
                     /translation="MDTKKVTFSSICIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            8804..9325
                     /locus_tag="I9573_001548"
     CDS             8804..9325
                     /locus_tag="I9573_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAT4796174.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFNEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            10116..12194
                     /locus_tag="I9573_001549"
     CDS             10116..12194
                     /locus_tag="I9573_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAT4796175.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            12194..12973
                     /locus_tag="I9573_001550"
     CDS             12194..12973
                     /locus_tag="I9573_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT4796176.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            12966..13775
                     /locus_tag="I9573_001551"
     CDS             12966..13775
                     /locus_tag="I9573_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAT4796177.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            13795..14277
                     /locus_tag="I9573_001552"
     CDS             13795..14277
                     /locus_tag="I9573_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4796178.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            14298..14702
                     /locus_tag="I9573_001553"
     CDS             14298..14702
                     /locus_tag="I9573_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT4796179.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANDDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(14858..16018)
                     /locus_tag="I9573_001554"
     CDS             complement(14858..16018)
                     /locus_tag="I9573_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HAT4796180.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            16232..17776
                     /gene="xylB"
                     /locus_tag="I9573_001555"
     CDS             16232..17776
                     /gene="xylB"
                     /locus_tag="I9573_001555"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAT4796181.1"
                     /translation="MEYVLGVDIGTSGTKTVLFDKLGNIIKSCTYEYPLIQEKSGWAE
                     QDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIWCD
                     QRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYINTNKILLPKD
                     YIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITGYV
                     TRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPKID
                     KEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIINNQ
                     VSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYSLK
                     DCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAILAL
                     VGAGIYENIQKACDAIIKTSTKLEPISENVDTYKIYHRLYKRIYKSLKDDFKLLDEVV
                     NTKINN"
     gene            17872..19209
                     /gene="xylA"
                     /locus_tag="I9573_001556"
     CDS             17872..19209
                     /gene="xylA"
                     /locus_tag="I9573_001556"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAT4796182.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNVTDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
BASE COUNT         7715 a         2028 c         3461 g         6119 t
ORIGIN      
        1 attttctaaa tcaaaaaaat aggttaagta aaggtgctac acaatcagct ttaaataatg
       61 aaggattagc taatataaaa atacgcatgt ataatttaaa agtgcaagaa aaggtagtgc
      121 gtgttttaga caaggctcaa gagttaatag ataaaagaaa agagcaaata gaagtattgg
      181 atgaattagt taaatcaaga tttattgaga tgtttggtac cccatcaaag aacgaaaaaa
      241 actgggaaat ttcagaaata gggaaatatt tagatgtttt aactgattat cattcaaatg
      301 gtagttatga aaccttaaga gataatgtaa ctcttttaga tacaaaaggt tatgccttaa
      361 tggtaagaac tacagattta gaaaataata actttgaaaa aggtgttaag tatatcgatg
      421 aacatgcata taattatctt gaaaaatcaa aggtgtttgg tggtgaggtt ataataaata
      481 agataggtag tgcaggtaaa gtttatttaa tgccattttt aaataaacca gtatctttag
      541 ctatgaatca atttatgctt agatttaatg aagataaagt gaatcatata ttcttatata
      601 accttttact aacatcctat atggaaagta aaataaaaga gaaagtaaga ggagctgtaa
      661 caaaaacgat aactaaagat gctgttagaa aaataaatat tattgtacct cctattagac
      721 ttcaaaatca atttgccaac ttcgttaaac aagtaaactc tttgaagttt gaaatggaaa
      781 ctagcttaaa agaactagaa gataacttta actcattaat gcaaaaagct tttaaaggag
      841 aattgttcaa ctgaaataca agtattaaaa tgggggaata aaatctatga atcaaataga
      901 gctagattta tatcatgaca tggtaaatat ctataaagaa gctgatttac aatgtaacta
      961 taagccaaca agatttttac aactgataaa taacaaagga gccttattag ctgctaaaga
     1021 attaatcaat aaagaaggag ctactgaagg atttacaagg ctatgggagt gtaagagatt
     1081 agatttatca ttagaagtat tagtattaaa agaaaagtat aaagagttgt ttactgatga
     1141 agaaagagaa atatgtataa atagattaaa agattatgga tataaattca attaagcaat
     1201 attacctaat aaacaagagg tgattaaatg agaaatatac caaatgagat taaaaactca
     1261 ataataaatg tatttccaaa agaaaatact ctagcaatca taatatttgg tagttatgga
     1321 acagataaac atacatcaga aagcgatata gacatagctt ggattcctat caaaaaagta
     1381 ccaattacag aacttgcaac taagacagaa gctctaagaa atatattaga cattgatgta
     1441 gacctaaaaa ttgttacaga taattataca atagcactta aaaaagctat tttagaaggt
     1501 gaagttattt atgaatctaa agattttaag gattatataa ataaattcta ttttgaaaac
     1561 agtgatgtaa tagatatctt agattggagg gacatgtatg gtaattagaa acaaattatc
     1621 ttggaactca atagaagaag taaataggga ttttggttct tgcttatatg acctgcaaaa
     1681 cttcaaaatc ttatatgata aggaagaaat gcctgagtta tgggctagat acataaaaga
     1741 aggttttaaa agctatatgg tctcattttt agaattaaca aaagcaatgt tgtattataa
     1801 atcaattgat ttaaacataa aatctaaaaa cttttatgac taccttttag cttgtgagta
     1861 tcacaattta ttacctaaaa attcttctat agttatagaa acacttagaa aattgagaaa
     1921 tgatgattct catggatatg atataccaca gtttgaagat atgtacgaat tgtttacaga
     1981 aaatgaagaa gtatttgtta gtataagaaa cagctgtaag aaatagtttt tagagagatt
     2041 tattttaact aagggacaaa agggggagaa aagccttggg taattttgac tttttacaaa
     2101 agaagaaaaa tttcaatagt tttaatactg cttgcttaga agcagaaaaa agtatactag
     2161 ttagcccatc tacttgtgca actataacaa gaagagcttt agagcttgca gtaaaatggc
     2221 tatatgctaa tgatagtgat ttagtattgc cttatcaaga taatcttagt acgttgattc
     2281 ataataatag ttttatagaa cttatagact atgatatgtt acctttatta aaatatatag
     2341 ttaaattagg taatttatct gtacatacta gtgcgaacat agagagagga gaagcaatac
     2401 tttcacttaa caatttgcat caatttgtta gttggataga ctattgttat tcagatgaat
     2461 atacagcaga agactttaat gaagatttat tacttcatgg ggaagagaaa aggacgagac
     2521 cagacgagct taaaaatctt tatgagagat taagttcaaa agataaaaag cttgaagaag
     2581 taataaaaga aaatgagaaa ttaagaaagt cactaactga aaagagaaaa gttaatactg
     2641 taaactacga tttcaagata gacgaagtaa acgaatttga gacaagaaaa agatatataa
     2701 atatagaatt aaagcttgca ggttgggaat ttggaaaaga tataggggag gaaatagaag
     2761 tacaaggtat gcctaatgaa agtggtgtag gatatgtaga ttatgttctt tatggtgaaa
     2821 atggtaagcc attagcagta gttgaggcga aaagaacgag caaagacccg aaaattggtc
     2881 aacaacaagc taaattatat gcagattgtt tggaaaagag atatagtcaa agaccagtaa
     2941 tatttttgac gaatggtttt gatatgtata tttgggatga ctattcagat agaaaagttt
     3001 atggatttta taaaaaatca gaattacaac ttatgataga tagaagaaag agtaagaagt
     3061 ctttaagttc tgtaacaata aatgatgaaa tatccaatag atattatcaa aaagaagcta
     3121 ttagggctgt atgtgaggct cttgaaaata agcaacgcaa aactcttttg gtatgtgcta
     3181 ctggtacagg aaaaacaaga attgctatat ctatagttga tgtactttca agacataatt
     3241 ggattaaaaa tatattattt ctagcagata gaaaagcatt ggtaaagcaa gctaaaaaaa
     3301 gttttactaa gctattacct aacttggcgt tatgtaattt attggatagc aaagacagcc
     3361 cagaagatgc taggatgata ttttctacat atccaacaat gatgaatgca attgatgata
     3421 cgaagtctaa ggatggaaag agattgttta cacctggtca ttttgattta attataatag
     3481 atgaatcgca tagaagtatt tataaaaagt ataagtcaat ctttgattat tttgattctt
     3541 acttaatggg tcttactgct acaccaaagg atgagataga taaaaatact tattcagttt
     3601 ttgacatgga aaatggagta cctacatatg cttatgagta taataaagca gtcgaggatg
     3661 gatatttagt agattatact agtattgagt ttaagactaa gattatggaa gatggaatta
     3721 agtatgatga gctttctgat gaagagaagg aagaatatga aaatacattt aatgatgatg
     3781 agagtattgg agacgaaata gggaataatg cagtaaatga gtggctattt aattctgaca
     3841 caatagattt agttttaaat aagctaatga cagaggggtt gaaaatagaa ggagaagaaa
     3901 agattggtaa gactataatt tttgcaaaga atacaaagca tgctagggca atagttgaaa
     3961 gatttaataa actgtatcca aagtatggtg ggaactttgt aaaagcagtt gactatagta
     4021 taaattatgt agattctata atagatgatt tttctgataa gaataaatta cctcaaatag
     4081 cagtttcagt agacatgctt gatacaggaa ttgacatacc tgaaatccta aatcttgttt
     4141 ttttcaagaa agtgagaagt aagactaagt tttggcaaat gataggtaga ggaactagat
     4201 tatgtccaga tttattaggt gttgatatgg ataaggaaag attcttaata tttgattttt
     4261 gtaataattt tgaatttttt aaatttaatc ctaaaggatt tgagggaaat aaagcagaaa
     4321 cacttactga aaaattattt aatataaagg taagtatggt taaagaactt caagatatta
     4381 aatatataga aggtaagtat gcagaactta gaaaagaatt gttagaagaa cttataactt
     4441 ctgtaaaagc tttaaatgag gatagttata tagttaggat gaatttaagt tatgtacata
     4501 agtataaaaa tgaaaatgtt tggagcaata ttggagcagt agcacaaaat gagataagag
     4561 aatatatatc accactcata acttcatata gtgatgatga attggctaaa agatttgatg
     4621 tagttatgta taatatacaa cttgcatact tacaaaataa taatgcaagt aaagggataa
     4681 ggcatgtaat ggttacagca gaaaaacttt cgaaacttgg gactatacca cagatccagc
     4741 aacaaaaata tactattgaa aaagcaatga cagaggattt ttgggaagat tcagatatat
     4801 ttgaggtaga agaagtacgt atatcattaa gagagcttat taagtatctt gaaaagtcta
     4861 gtcaaaagat atattataca agttttgaag atatgattgt agcagaagat agaaatgatt
     4921 ctgtatataa tgcaaacaat cttaagaatt ataaaaagaa ggtagagtat tatttaaata
     4981 gccataaaga tgaattagca atatttaaat taagaaataa taaaaagata acaaagcaag
     5041 atgttgaaac gttggaggaa atactcttaa agcagttagg taattgtgat gattataaaa
     5101 aggagtttgg agatactcca gtaagtcaat tagtgagaaa actagtggga ttggatagag
     5161 aagctgcaaa tgaagctttt tcagagtttt taaataataa gagttttaat actaagcaaa
     5221 tacattttgt taaacttata gtcgattatg tagtcaagaa tggatttata gaggataaca
     5281 aagtgttgat ggaagaccca tttagaactg ttggaagtat aattgattta tttgaaaatc
     5341 atattgaaga gagaaataaa ttgattaaaa ctataaataa aattaaagaa aatgcatcag
     5401 agataggcta atgatgttat ttagtgcaaa attattgtta attatgtaga gaataaattt
     5461 attagtaggt agtctaatat tgggttgaaa tagataacta gatatggata atgtaaatat
     5521 acaaagagag gtattgaata attatgcaat atctcttttt aattgataaa taactatgaa
     5581 ttactcatga agaagattaa ataataattt ttaaaattaa gtatttgtat attttataga
     5641 ataatcatgt atttacttta ataaaataaa aataggtata aaaaggtata taataaggct
     5701 ttcatataca tttatgagtc aaaattttga tataatatat agatacatgg agttatgaat
     5761 ggaatttatt taacttatag ggggagttaa tatgaaagca ggagaaattg aatttcttag
     5821 ttatttagag gggtcaaaca aaagttttgt agtgcctgta tatcaaagaa attataactg
     5881 gaagaaagaa caatgtaaaa gattgtttga tgatttagag gatataatag attctggatt
     5941 tagaactcat ttcttagggt cagttgtatc tatatatagt atgggaaaag aatatttaat
     6001 aatagatgga caacaaagag tgactactgt gtcaatatta ttgattgcca tgtgtcaaat
     6061 aataaaagag tttggagaat caaaaggaat tattacaaca gaagaacaga ttaaggagtg
     6121 ttatcttata aacaagcact caagagataa agatggaaaa ataaagttaa aaccaatagc
     6181 agaagataga aaagcttact atgaactatt tgaagaagaa atgaatacag atagaaattc
     6241 taatatagtt tcaaattatt tatattttta taatagaata aaagaagata atatatcaat
     6301 agatgatttg tttgatgcta tacagaaatt aactatagta gaaatagagt taaaaaatgg
     6361 tgaagatgac cctcaattaa tctttgaaag tctaaattca actggcttag atttaagtga
     6421 agctgataga gtaagaaact ttgtgttaat gggtagaagc tctaagttac aagaggaatt
     6481 ttataataat tattggtata aaattgaaaa aaatacaagt agcaacgtaa gtagctttat
     6541 aagagactat ttgactataa aggagagaag tataccgaat aaaaataaag tatattttag
     6601 ttttaaaagg tatgtatcag ataatggtat agatatagaa attttattaa aagatttact
     6661 taaattttct aactattata gagagatatc ttattcatgt actgatgata atgatgtaaa
     6721 caaagttttg aaaaggataa acaaacttga aatatttgta tcatatccgt ttatattaga
     6781 attatttaat gattataatg aaggtataat aaataaaaaa gtacttgtag aatcccttaa
     6841 tttagtagaa tgttttatat taagaagaat agtatgtgat gtacctccga atgcactgaa
     6901 taaagtattt atgaacttag gaagggaaat taaaaaattt agtgattata aagaaaacta
     6961 ttctaatata ttaagatata tttttataaa taagaaatca tctcaaagat ttcctactga
     7021 cagtgaattt tcaaatgctt ttgaaactaa agatatatat aatactaagt caaagaataa
     7081 gttatatata cttgagaggt tagagaatta taataataaa gaaattgttg atttagaaaa
     7141 tttaatcaat aataatatgc taaatataga gcatataatg ccacagacat taacaaataa
     7201 atggaaggaa tcactaggag ataactataa ggagatacat gataaatatt tacatacaat
     7261 tggaaattta actttgacag gatataattc aaaactaagt aataaaacat ttgaagaaaa
     7321 gaaagaaatg gaagatggat ttaaagatag taggctttat ctaaataaat atatttcatc
     7381 tatagataaa tggaatgaag aagaaataaa aaatagaact aagatattac tagatagagc
     7441 tgtagaaatt tggaaatatc caagtgttac atatgaacct ccaagtaaca gtgaaaatat
     7501 atttacgtta gaggatgatg atgttaattt tacaaatgaa agtatagtat catttagtat
     7561 attaggagag tcatataagg ctaaaaattg gacagaattt tatgaacaag ttgctcttac
     7621 tttatatgat ttggattctg taaggtttaa cagtattgta gacaagactt ataaaagaga
     7681 ttttatagat aatattaagt ctagagatgt atataaatta agaaaaggta ttaaaatatc
     7741 agaaaatata tatttagaaa caaatttaaa tacagaggct aaattaaata taattagatt
     7801 tttacttaat gagtttgata tagagttaaa tgaagtaata ttttccataa aataatgaaa
     7861 tatatgcatt ttttgcgtaa tagaatatta attatattaa aaataaaata gcacctcttg
     7921 atatagattt atagctcaaa tttatatcaa gaggttacgt ttaattaaaa taatacaaat
     7981 actatctaat taaattatta ataaaaatgt tatataaaaa tgagtatata gcattttaaa
     8041 gagtaataaa tcttacataa taaatgattt attaatatga actagaagta tttttactat
     8101 attgttttat aaaaccttta ggcattttac ttataaaata taccaccata atacaagaca
     8161 aaggtttgtc tactatatta ctaagaatcc ttggaaagaa cgcagcagta aatatcttct
     8221 gaccagactg aactaaaaaa ccaaccaata aatcagcacc acttccactt aaaccaccaa
     8281 acaataaaac tgaaatagga gtaccaatta aaggacaaac aatagataaa attactcctg
     8341 taataatagc ttctttataa ccaaaacctt ttttcttaga aatatatcca acaataagac
     8401 caagaacaat atttacaaga gcaaaaggta tatttgtagg acctgatata acacctaaaa
     8461 ctaagtttgt acacccacca ataagagcac cccaaaaagg acctaatacg acagccccaa
     8521 gtacagtacc aactgtatct aaaaataata atggtatatt tatcatagat ataaccatac
     8581 ctaagacaat attaataact atgcatattg aagaaaatgt gactttttta gtatccaatt
     8641 ataatacctc ctaaaaaaat atatacattt atcatagtat agattataaa agattactaa
     8701 ttgtatattt ctatatagaa tattggaaat atagaacaaa taaataatat aattatcaaa
     8761 atatgtagta tcattaaaat taagaataac taaggagatt attatgaaga gaaatagaga
     8821 gattgtagta gtatgtcatt gtatattaaa ttgtaattct aaagtagagg gattgtcaga
     8881 atttaaggga gcacaaaata tagcgataaa tttaataaat aaagggtatg gaataattca
     8941 actaccatgc ccagaaatga ttatgtatgg aataaatagg tggggacatg taaaagaaca
     9001 gtttgacaat ttgtttttta gagaacaatg taggcaaatg ttaatacctc atgtaaggca
     9061 gtttaatgaa tatttaaaaa atgattatga tataaaaagt atcattgcaa tagatggtag
     9121 tccaagttgt ggttataata agacatgtag ttctaaacgt tggtttggtg agctaagtgg
     9181 ttgcaatgaa ttgatggata aaataaatga tatacaaatg gtggatggga aaggtatttt
     9241 tatagaagag ctagaaaagc tacttaaaga atataattta gagataccta tagttggatt
     9301 tgacgagctt agagaagata agtaaagata taaatattta ttgtctcatt atgaaacacc
     9361 attatatttg gcagaaaaat atgactgatg gacagtacct gaatcatttt taagtggagt
     9421 aattagtact tataaagatg tatctactag ctgaaagaaa atgtagagag gtaataaatg
     9481 aattgataat atatataaag gagtgtctaa aagtgaactt tttagtttat gagacactct
     9541 ttttttatat taaatcaagt atattaaatc aaatatattt taatcatttt agcaatgaaa
     9601 aataaattta tctatatttt gagacaacat cttttttagt tttatttatt tgttgtcgtt
     9661 tttgacttgg tatacaatta tatattttct taaataattt cataaattat ttataattat
     9721 aaaatccatt gttttgaaca atatctaata tattattatc tgtatttata aggtcactat
     9781 acatattttc aagtccaata ttatctacat aataggcaaa ttttattcct gtatgtgttt
     9841 aaaaaaaact agatgtatat aggtatcatc acagatatta aaagtataaa taaaagatat
     9901 taagaacgac tttcaagcaa catattagaa aattttacta aaatccggaa agcgattgac
     9961 aaacaaagtc aaataaagta tattattggt aagttagttt aatgtacaaa tgaattatat
    10021 ataaaaccta atttataaat aacgatacta aacgacatta tttaacttaa gctacacaaa
    10081 gatagaatat tataccattg atttaggagg tagccgtgat aaaaatatct gaagactata
    10141 ttgaaaaaag atttgacaat gaactattgc gaatagaagc atggggaaaa aactctttac
    10201 gtattaggtc gtttgtagac caaaattttg ttgatgaaaa ctatgctctt aacgaaaaac
    10261 ctaagttaaa taaagatgat attacaatta ataaaaatga agatggaagt gcaatcatta
    10321 aaaatggaaa gataaaagct gtcttagacc atagagatag aattactttt tataatgata
    10381 aaaatgaaat attattgaaa gaatatattc gattaagagc tgtaaaacat gatgatggtg
    10441 gagaagatgt agggacaatt gagattacca aagactttaa ctctactcta aagttaaaat
    10501 caagagaata tcgaccaagc tatagtggag aatttgaagt aactactaga tttgaatctg
    10561 agccttctga aaagattttt ggaatgggac aatatcaaca taaattttta gatttaaaga
    10621 atactgtttt ggagttagcc caaagaaatt ctcaaatatc agttcctttt tatatttcta
    10681 gtctaggtta tggactttta tggaataatc cgggaattgg aaaagtatct tttgctaaaa
    10741 atatgacaga atggaagatg ttttctacaa attttataga ttattggatt acttgtggag
    10801 aaagcccaaa agagttaaat aaaaattatt ctcaagttac tggaactgtt cctatgatgc
    10861 ctgaaaacct tttaggacta tggcaaagta aactaagata tagaacttca gaagaagttt
    10921 tagatgttgt gaaggaatat tctaaaagag gaattaagct ttcatcaatt gcaattgact
    10981 attttcattg gcctaagcaa ggtgaatata aatttgactt agattattgg aaaaatccta
    11041 aagaattagt taaaaaattg aaagaagagt attcagtaga accaatagta tctgtatggc
    11101 ctacagttca aagtgatgca gaaaactaca atgattattt agaaaatggc tatttagtca
    11161 atgttaatcg tggtgttaga atgacaatgc aaattcaagg aaatacagtc tttgtagata
    11221 tgacaaatga aaatgcaaga gaatatgttt gggatagaat agataaaaat tataaacaac
    11281 ttgggattga ctattattgg ttagatgtag ctgaacctgg ctattctgtg tatgattttg
    11341 ataactatag atataaaaaa ggaaatgttt taagttgtgg taatatttat cctattgatt
    11401 atttaaaaat gatttatgat ggtttacata atgacataga gtctgttgta actcttgtaa
    11461 gaggagcttg ggcaggagct caaaagtatg gagctttagt atggtctggt gacattgatt
    11521 ctagttttga ggcttttaat aatcaagtaa atacagggtt aaatatggga ctggcaggta
    11581 taccatggtg gactactgat attggaggtt tccatggagg aaatcctaaa gacccagaat
    11641 ttagagaact tatggtaaga tggttccaat atgctacttt ttcaccaatt ttgagaatgc
    11701 atggagatag attgcctcat agtaaaccat tatcaaataa aggtggaggc tcaatggtaa
    11761 ctggagctcc taatgaaata tggtcttatg gtgaagaagt tgaagtaatt cttacaaaat
    11821 tcataaaaat tagagagagc ttaaaaactt atttaaaaaa actgatgaaa gaagcgcatg
    11881 aagatggaac accagttatg agaactttat tttatgaatt tcctgaagat gataaaactt
    11941 gggaggtgga taatacatat atgcttggag atgaaatttt ggtagctcca ataatgaatt
    12001 ataaagatag aagtagaaaa gtatatttac caaaaggaca tacatgggaa aatatattta
    12061 gtggagttag ttatgaaggt gggaaaactt atgaagttga atgcccgctg gaagaaattc
    12121 ctattttcct aaagcaagat tccagttata attttaaaga actaaaaaat attttgggag
    12181 aggtgaaatc ataatggagc cacaattatg gcaattatta ttaatcgtac tatatggatt
    12241 ttttataaac tatgaaaaaa attcaacaat gtttggaaca tatcaaccag taacagcagg
    12301 ttttattact ggtcttatct taggagatat aaatacaggg ttatatattg gaggtacatt
    12361 acagctatta tcattaggta ttagtaactt tggtggagca tcaatacctg attatcaaac
    12421 agcaagtata gtagccacat ttataactat tacgactaag caagaagcat ctgtaggaat
    12481 ctctataggt attccagttg ctttattgat ggtacaatta gatgttctta gaaatactat
    12541 tggtatatgg ctagttcata aagcagaaga tggagctaaa aaaggaaatt ataaaaatat
    12601 aacctatatg caaatgttag gagtactatt aacagcagct acaacaggta ttccagttgc
    12661 tctatctgta atatttggac cttctttaat taataccatt ttgaaatata cacctgaatg
    12721 gttaacaggt ggtttaacag tagctggagg actacttcca gcagtaggga taggattatt
    12781 acttagatat cttcctgcta aggagtattt tagttactta gtaattggtt ttgttttagc
    12841 agtatatatg aaagtaccat tattaggtgt agctttaatt ggtggagcaa ttgcacttat
    12901 aatttacaaa aagaatttgg aaaatcaaga acaacaatat actgtagtag gaggtatgga
    12961 cgaagatgag tgataaggta tcattaaata aatcaaatga aaaggtgaaa aaaagtgtct
    13021 tatggcgctg gttttttact agttctgttt cttcaaatta tgagaagatg caggccttgg
    13081 catactgtta tgcagtacta ccttttttaa aagtagtata caaagataat ccagaaaagt
    13141 tacaaagtgc tgtattaaat catttacaat tttttaacac taatccttgg gttgcccctt
    13201 atattttggg tattaatgta gcaatggagg agttatcaag tgaagagact gaagaggctg
    13261 ttgcttcaat taaaacaggc ttgatgggac cagtagcagg tttaggagat agtttatttg
    13321 tcgttattcc gtggacaatt tttggagcta tagcagctaa tatggcgata gatggaagtc
    13381 catttggaat cttactgtgg attgtagtga gtgtagcatt gaaattatta agtataccac
    13441 tatttaatgc tggctataca tcaggtacta agctaatatc ttcaattgaa aaaagtttaa
    13501 agattttaac tgagagtaca tctattttag gtttaatggt tgtaggtgca ttaatacctt
    13561 cagttgtaaa agctaatgtt gctttgaact ttcaacaagg tgattttaca atgaaaggtc
    13621 aagaaatatt agaccaaatt atgcctgggt tagttcctgc aattttagtt gtgattgttt
    13681 attgggctct taaaaagaat ataaaaccta tatacttaat tttaggagta atggttgttt
    13741 caataatttt atatgcatta ggtattttaa aataaggaaa ggatgatatt agatatggaa
    13801 atgaaaggta ttaaaaatat tcgtatagat gaccgtttaa tccatggtca agttgcaact
    13861 atgtggagta ataaattggg tgttacaaga ttaatggtag taaatgatgc agtagctaac
    13921 aattctgttc aaaaacaagt tttaagaatg gctacacctg caggtatagc gtcatctata
    13981 attacagaag agactgctat aaaaaatatt actgcaggaa agtatgaagg acaaaatgta
    14041 ttattaattg ttaagtctcc tgtggacctt attccatttg tagaggcagg atttaaaata
    14101 cataatataa atgtaggaaa tatgtcaagt agaaaagata caacagttct aagacctaat
    14161 atcagtgtta ctgaagaaga gaaagaagca tttaaaagac ttttagatga aggaattgaa
    14221 attacaacta taatgacgcc tgatgataag aaaacttatc ttgtagacat cttatagatt
    14281 taaagaggtg tattaaagtg aatagtatta ttttgatgag tcatggcaat atggctgaag
    14341 aagtcttaaa ttctgcaaaa atgataatag gggattctat agattatcct actgtaaata
    14401 tgaaacaaga tgatggaata gaaggaacaa tagaaaaatt aaaaaaagct ttagaagtgt
    14461 acaaggaaag ttcagaaata attataatgg cagatttact aggtggaact ccatgcaatg
    14521 ctgctttatt agtagccaat gatgacagtc gaattaaaat tatttcaggg ctaaatttag
    14581 gtatggttat tgaatcagct tttttagata caaaggaatt ggcagcaaaa ttagagaaaa
    14641 taggtaaaga taatatttct atggttgaac aaaatgcaag tttattatct gatgatgagt
    14701 aggaagatta tattaatatt taaaacaaaa tacttgtgtt aatacttaat tgatatacaa
    14761 tttttaaaaa atatagctaa tcttgcataa actatataat aaatctaatt ttagaatgta
    14821 taatttaaag ggttatggct gtatatagtc ataaccctta tatttcatta attaaagtta
    14881 aattttgtat attcaaaaag ctcttgatat ttacggcaga gccaccatat agagatgcct
    14941 cagaccctaa tgatgaaatt tctatattta ttcctttgtt aaaagagcta actaacatat
    15001 cttttgtaat ctgtaatatc tcagggatat cactaattat ttggctattt aaatatatta
    15061 tttcaggagc ataagttgta atagcattat ttatagctat tgttaagtaa ctacaaaact
    15121 catgaataac ttttttggca ttctggttat cttcataata tagttgcttt actatatcag
    15181 aatctatttt aggaatattt tctaaagaag aaagctgttc aaataccttt ttttctgaac
    15241 aatactgttc taaacagcca cgatttccac aaggacatag tttaccatta ggcatgatga
    15301 tagtgtgacc aatttctcca ctcataccat ttctgccgct atataattta ttatttatta
    15361 taatgcctga accaaatcca ctgtgaatac tgagactaag tagtgagtta tgtacagttg
    15421 aaaaagtatt ctcagctaaa gctgttaagt ttgcttcatt ttctatatga ataggaaagt
    15481 catacttttt gctaaggatg ctatataagt caatctcatt aagattataa tatggagtaa
    15541 acaatacttt attttcacaa gtaatcccat gtatagccaa agtaagacca attaccttat
    15601 aaggagtgtc tattttggat atgttataac tatttataat ttcatctatc aattgtataa
    15661 cattgtcttt acttacttgt atatctgtta atttcttaga atttataata gttccatcta
    15721 agtaagatag agaagaaaat atatagtcgt atccaatgtc catacttaaa gaaatccctg
    15781 cacatttatt aaatactaat aatatgggtt ttcttccgcc actatgagta ctatttccaa
    15841 ttcctatttc atgaactaga gattcatcaa taagtttttt ggtaatagcg gatatagttg
    15901 ctttattcaa tcctatagta gaagctatac ttgccctaga aataggacca ttttttataa
    15961 tttgttcaag taccaatctt tcattcattt ctcgaatagt gtatttatca gtaaccaatt
    16021 tgatattcct ccttaaaata atattgtaat actttttaca caaaaataaa aggttatttt
    16081 gcattgacaa agataattaa atattttatt attagttcat aagttagttt aatatactaa
    16141 caaaaataaa gcaagtaaaa tatacctaaa atataaaaaa attaggatag gaaaacgata
    16201 gttatgaagt ggcattcaag gagggatgaa gttggagtat gtattaggtg tggatattgg
    16261 tacatcaggt actaaaacag ttttatttga taaactagga aatattataa aaagttgtac
    16321 atatgaatat cctcttatac aagagaaatc aggttgggca gagcaggatg caaatgattg
    16381 gtggaaagct gtagtcgaat ctataagaga agtagttcaa agttcaaata taagcagtga
    16441 atgtataaaa ggaattggat tatcagggca gatgcatggt ctagttatgc tggacaatga
    16501 aggtaaaaca ttgagaaact ctataatatg gtgtgaccaa cgaacagtaa aagagtgtga
    16561 ggagataact gatttagtag gtgaagaaag actgattgaa ataactgcta atccagcgct
    16621 tacaggattt acagcatcaa agatattatg ggttagaaac aatgaaccag atatatatat
    16681 aaatactaat aaaatacttc ttccaaaaga ttatataaga tacaaattga caggtgaata
    16741 tgctacagag gtatcagatg caagtggtat gcagttgctc gatataagaa aaagagattg
    16801 gagcgatgaa gtactagaaa agttaaatat agataaaaat cttttaggta aagtttatga
    16861 atcacaagag attacaggtt atgtgactag agatgtagca agtttgacag ggcttaaaga
    16921 aggaacaata gtggtaggag gagctggaga ccaagcagca ggagctatag gaaatggaat
    16981 agttaaagat ggagtagttt catctactat aggtacatca ggtgtagtat ttgcttatac
    17041 gaaagagcca aaaattgata aagaaggtag agttcatact ttctgtcatg caataccaaa
    17101 tacatggcat gttatgggag ttactcaagg agctggattg tcattaaaat ggtttaaaga
    17161 taatttctgt caaagtgaag tggaagtttc aaattcttta ggtgaagatg tatatgagat
    17221 tataaataat caagtaagtc aagtaccaac aggatgtaat ggattgcttt acttaccata
    17281 tatgatggga gaaagaactc ctcatcttga cccttatgca agaggtgtgt tctttggatt
    17341 atctccaatc cattctaaaa aagaaatcgc aaggtcaata atggaaggtg tttcatatag
    17401 tcttaaagac tgtatggata taatagagaa tcttaatata gacgttaatg aagtaagagc
    17461 ctcaggtgga ggtggaaaaa gtaaagtatg gagacagatg caagcagata tgtttaatca
    17521 agatgtatat actataaatt caagtgaagg accagcactt ggagttgcaa ttcttgcatt
    17581 agtaggagct ggaatatatg aaaatataca aaaagcttgt gatgctataa taaaaacttc
    17641 aactaaatta gaaccaataa gtgaaaatgt agatacatac aagatatatc atagattgta
    17701 taaaagaata tacaaatctt tgaaagatga ctttaagtta ttagatgaag tagttaatac
    17761 aaaaataaat aattaaatta gcttaaaaag aaaaataaag atagaaaata gttttaaatt
    17821 aagaaaacat acgacatata ttatatgtgt aaaatattag gaggacatga aatgagtgaa
    17881 atatttaaag gaataggaca gataaaattt gaaggagtaa aatctgacaa tgaattagca
    17941 tttagatact acaatcctga acaagtggta ggtaataaga ctatgaaaga acacttaaga
    18001 tttgcaatga gctattggca tactttatgt ggagagggta atgacccatt tggagtaggc
    18061 acagtagaga gaccttggaa taatgtaaca gacccaattg agatagcaaa gattaaagtt
    18121 gatgcaggat ttgaatttat gtctaagatg ggaattgagt atttctgttt tcatgacaga
    18181 gatatagctc cagaaggaag agatttagaa gagacaaata agatattaga tgaaatagtt
    18241 gagtatataa aagttaatat ggaaaagaca ggtataaagt tattatgggg aactgccaat
    18301 atgtttggaa atccaagatt tgttcatggt gcatctacaa catgtaatgc agatgtatat
    18361 gcatatgcag cagctcaggt taaaaaagct atggaaataa ctaagtactt aggaggagaa
    18421 aattttgtat tctggggtgg aagagaaggt tatgaaactt tacttaacac taatacagaa
    18481 cttgaaatgg ataactttgc aagattctta caaatggctg tagattatgc taaggaaata
    18541 ggattcactg gccaattctt aatagagcct aagccaaaag agcctactaa acatcaatac
    18601 gattttgata ctgctactgt tttaggattt ttaagaaagt ataatctgga taaatacttc
    18661 aaaatgaata tagaagcaaa ccatgcaaca cttgcaggac atactttcca acatgaatta
    18721 aatatagcaa gaataaacaa tgttttaggt agtatagatg caaatcaagg agacctatta
    18781 ttaggttggg atacagacca atttccaaca aatatatatg atgcaacact tgctatgtat
    18841 gaagtgttaa agcaaggtgg aatagcacca ggaggattta actttgactc taaagtaaga
    18901 agagcatctt ttgaggtaga agacttattc ctagcttata ttgcaggaat ggatacattt
    18961 gcaaagggat tattaatagc tcataaatta ttagaagatg aggtatttga aaactttact
    19021 aaagaaagat atgcaagttt tagtgaaggt ataggtaagg atatagtaga aggtaaggtt
    19081 ggatttaaag agttagaatc ttatgcactg caaatgccag ttataaagaa taagtctgga
    19141 agacaagaaa tgctagaagc tatacttaac agatatatat atgaagtaga tacaatttct
    19201 aataagtaat tgtgtagtaa ttaaacttgg atgtttgaat aaaataacag gatatgttct
    19261 tgtttttatt ctaaaacgga caaagataag tatttgtaaa aattctcata atcatgataa
    19321 att
//
LOCUS       DACTGW010000056        19322 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.056, whole genome shotgun sequence.
ACCESSION   DACTGW010000056 DACTGW010000000
VERSION     DACTGW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.056"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     repeat_region   1..621
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1..23
                     /rpt_unit_seq="attccatatagttaatataaaac"
     gene            complement(1452..2324)
                     /locus_tag="I9573_001557"
     CDS             complement(1452..2324)
                     /locus_tag="I9573_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT4796183.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(2718..3296)
                     /locus_tag="I9573_001558"
     CDS             complement(2718..3296)
                     /locus_tag="I9573_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAT4796184.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(3354..4070)
                     /locus_tag="I9573_001559"
     CDS             complement(3354..4070)
                     /locus_tag="I9573_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAT4796185.1"
                     /translation="MRINKYIASCGIASRRKSEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            4261..5346
                     /locus_tag="I9573_001560"
     CDS             4261..5346
                     /locus_tag="I9573_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAT4796186.1"
                     /translation="MIIILKENIKYYLIVVFIGILFFKFINTPSNFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(5501..6160)
                     /locus_tag="I9573_001561"
     CDS             complement(5501..6160)
                     /locus_tag="I9573_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796187.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KYSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6377..7669)
                     /gene="lysA"
                     /locus_tag="I9573_001562"
     CDS             complement(6377..7669)
                     /gene="lysA"
                     /locus_tag="I9573_001562"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAT4796188.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(7687..8880)
                     /locus_tag="I9573_001563"
     CDS             complement(7687..8880)
                     /locus_tag="I9573_001563"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAT4796189.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(9241..9858)
                     /locus_tag="I9573_001564"
     CDS             complement(9241..9858)
                     /locus_tag="I9573_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796190.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(10028..11728)
                     /locus_tag="I9573_001565"
     CDS             complement(10028..11728)
                     /locus_tag="I9573_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796191.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(12125..12688)
                     /locus_tag="I9573_001566"
     CDS             complement(12125..12688)
                     /locus_tag="I9573_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796192.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(12847..13560)
                     /locus_tag="I9573_001567"
     CDS             complement(12847..13560)
                     /locus_tag="I9573_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HAT4796193.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            13852..15516
                     /locus_tag="I9573_001568"
     CDS             13852..15516
                     /locus_tag="I9573_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HAT4796194.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDSKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(15623..16522)
                     /locus_tag="I9573_001569"
     CDS             complement(15623..16522)
                     /locus_tag="I9573_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAT4796195.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(16549..17187)
                     /locus_tag="I9573_001570"
     CDS             complement(16549..17187)
                     /locus_tag="I9573_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HAT4796196.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(17523..18521)
                     /locus_tag="I9573_001571"
     CDS             complement(17523..18521)
                     /locus_tag="I9573_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HAT4796197.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(18619..19194)
                     /locus_tag="I9573_001572"
     CDS             complement(18619..19194)
                     /locus_tag="I9573_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796198.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         6294 a         3231 c         2013 g         7784 t
ORIGIN      
        1 attccatata gttaatataa aacagttgtt tttatttttt ctaaagagaa aaaactagac
       61 tttacattcc atatagttaa tataaaactt tctgtatatt ttagttgtgt tttaatatta
      121 taacacttta cattccatat agttaatata aaacatggca gtaactaaga agaaaactat
      181 acctttttta ttctttacat tccatatagt taatataaaa cgaggaaatt ttatactaat
      241 gactgatgct agatttaact ttacattcca tatagttaat ataaaactat tataaggagc
      301 attatacgac caaaagccaa agcagcttta cattccatat agttaatata aaacaagcaa
      361 cagcaagagt cgcgacaaca ttaaaaactt cctttacatt ccatatagtt aatataaaac
      421 ctttattttt ttctctatat cgagttcgcc aaattcactc tttacattcc atatagttaa
      481 tataaaacag tccagcgttg tgtggtcgta acacaccaat cctactttac attccatata
      541 gttaatataa aacttataca aacgaaaatg aacttgtttt ggataattgt ttctttacat
      601 tccatatagt taatataaaa ccccaaaata aattaagtat ttccaatact tatacatata
      661 caattctcct aaacttgcag tgagcgagca atagtgcaat tgataacagt tatcatacac
      721 tctcaacgct ttatatttca actattaaac cctactttat cacaaaaatc gctcactgca
      781 aaacttttat atttttatta tatcataaaa ctcatactat aaaataaaaa tgggataatt
      841 ttacaaacta aaagcatcta cattcttata agtatttatt atattctcta ttccaatgat
      901 tcctttttct ttttattgca tccttcctat gtaatcgcac tttagctcaa caagtctttt
      961 attttcatta acaatttttt aaactattca aaatcatcaa atttttcatc ttatacagtt
     1021 aaatcaaaat acgtgttata tctctttatc tatatacttt tcttccttcc atttcttttt
     1081 ctctttctac attttcactt tgttacttta caatctcaag gttattatct gctattaaag
     1141 ttttatcttt ccaggtttca caaaaataaa ttgaataagt gcaaaaataa cagatacaaa
     1201 accataacta ttttcttttc tataaaaaaa tatatcatca acaagaaaca taataaatga
     1261 aaataactat gaataaatac tctcatttag aagaaataga caaagatatt ccttaaaatt
     1321 taagcaaaat attgtttaaa taaaaaaact gactgtcagt taaatgccag tcagctattt
     1381 tatatatttt atccaaacat cactatattt gaacatttta ataattaata atacttatta
     1441 atatacaaac tttactcata cacagaatat cttttccaaa tttgctctaa atcatcaaaa
     1501 tgctgtttaa tatcttcttt ttccttcatt ccaacagata tagcaagggc atttgaaata
     1561 ctaagagctg gaactagaga atctacaaat gaagccatat tactcttaac tagaagagtg
     1621 ttatctgcaa gtgatgctac tggcgcaaat aagctatctg ttaatgatat tacatgggct
     1681 cctttttctt tagcataatt gacaatttga taagatttct tagaataacg agggaaacta
     1741 atagcaacta ttacatcttc ttcatttatt cttacaatct gctcaaaagc atctcccata
     1801 tccattctaa ttatatgaac attgtcaagt attatatcta agtaaaaacc taaatattga
     1861 gctactacaa aagaactcct cattccaagt atatatattt ttctcgctct taaaagtttg
     1921 ttagaagctt ctttaaacgc tctctcgtca atttcttcta gtgtagacct tatattatct
     1981 atatcacttt ttaaaacttt gtttagtatt gcaaaatcat cggaataatc atgtgccatt
     2041 tcaactcttt gtactgtagt tagtttattt tttatgagtt cttgaagtgc agcttgtaat
     2101 ttaggatatc cactataacc taatgcattt gcaaatctta caactgtaga ctcacttact
     2161 cctacggttt cacccaattt acatgcagtc ataaatgcaa ctttatcata attatttaaa
     2221 atgtattgtg ctataagttt ttgaccctta ctcaatctcg tatattgaga ttgtatattt
     2281 gatatcaaat gtttactgtc cttcatttca tttgtttctt ccattttttt ctccttttag
     2341 ttatataaac tattttgttt tattaacact gtctttttct ctaaaaattt tattttttat
     2401 ctcaatttca tcttagtttt tatcatagct tcttaaactc ataaaattct aacttttctg
     2461 taccttatac ttcacatagt attttcgatt aaatttatta taccatcttt ccttgcattt
     2521 tttaatattt atccaatatt tcttttaaat ttttcaattt ttactcattt aaaagttatc
     2581 tatcttattt tgcatattta gatgtatatt ttgttttgag aataagctgt tgtatactta
     2641 ttatttaaag tatttttaat aggtcacact atatatatta actcttaata gaatcttgtc
     2701 aagtttttta gtatttatta tgcttttttc ttttcaatta aaatcaattg tggtgggtta
     2761 ttgacttgat ttaaaaactt agattctaaa acattaaatt catcttgatt taaattattg
     2821 acaaggttat atatgcaatc tttttcttct tgacctccat catgccctgt atatatggca
     2881 atgcttatta ctccattttc ttttaacatt tctaaactta actttatagc ctttaaagtt
     2941 gtatctggtt tggttataat tgaatgtttc gctcttggaa gataacccaa attaaataaa
     3001 acacatgaaa cttcttcttt tatatattga ttcatttttt catgcccatc aaatatcaac
     3061 tttactctat ctgtatatcc ttctttctct agtttctttc ttgtagactt tattgcttct
     3121 tcttgtatat caaaagcata tacaaagccc ttttctccaa ccttctcagc tagatacctt
     3181 gtatcatacc catttcccat agtagcatca attactatgt ccccttcatt tataatattt
     3241 tctaaataaa atttatttat atctgtaatt ttagttaaat actttgcata tgccataata
     3301 ctatttctcc ctactattaa ctttatttca ttttttctca atttattttt atcttatttt
     3361 attgatttta aatattttat ttcatcttct gttaggtatc tatattctcc aactttggtg
     3421 tcttttaata ctattttccc cacagaaatc cttcttaaat ttaaaactgg atgatttata
     3481 gctttgcaca tttttcttac ttgtctattt ctaccctcat gtatagttat ttcacacact
     3541 gaataatttt tttctttatt ttctttagtt acccttattt tggcaggtgc cgttgtgtaa
     3601 tcttctattt ttagtcctgt ttcgaattgt cttatctcat catcacttat aattcccttc
     3661 acacttgcca catacgtttt atcaatttca tgctttggat gggtaagctt atatgttaaa
     3721 tcgccatcat tagtaagtat taataaacca ctagtttcat aatctaatct ccctattgga
     3781 tatactcttt cccttacatc tttaagtatg tctattacac tgggtctatt gaattggtct
     3841 tttactgtag ttatatatcc ctctggttta tttaatacta tatatatata attttcactt
     3901 ggttttactt ttttattatc aaattctact atgtcttttt cttcatcaat attaaaagat
     3961 aactctttta ctacacttcc atttaccttt attcttcctc caagaattat ttcttctgat
     4021 tttcttcttg atgctattcc acaagaagct atatacttat taattctcat acttaatcct
     4081 tccttatctt aaattatgtg tgtttaatat ttttatcaat gttaattttg ctacattatt
     4141 atactatact agaccttaat taaaaacaag caaaaaaatt atttaatttt cctaaataat
     4201 tgtaatttct tttcacatac tattaatatg taatagctga ctaaacacta ttttaaggaa
     4261 gtgattatta ttttaaagga aaatataaaa tattatttaa tagtcgtgtt tattggaatt
     4321 ttatttttta aatttattaa tactccatca aactttatat ctagtattga aggattctta
     4381 aagtttttta gcccattttt attagctatt ttacttgctt tacttttaaa tccacttgtg
     4441 atgctttttg aggtaaaatt caaagcacat agacttttag ccattttttt atcttatata
     4501 tttataggtt ttattttagc ctttgccatt aggctattaa ttccatcaat agctaataca
     4561 ttaaatagat tgataaatga aatgcctatg tatactgatt atatagatag ttttatagaa
     4621 aaaaatatgt caaacataga ttttctaaaa actttaattc ctcatataca acatagttta
     4681 gataatttat taaaagaagc aagtaatttt gttggtaaaa ttcctaaaaa cttcttaatc
     4741 tatacactta gtatatcttc tatgctattt aatatgacaa tgggatttat actatccata
     4801 tacattatat atgacaaaga aaaaattgca ttgggattta agagatttct atattcttct
     4861 actgctagaa acaaggcaga taacattata gaatttttta ggactactca tgatattttc
     4921 tatgattatt tacttggtag aatattagac tctcttatta ttggtataat tgcattttta
     4981 ggatttcaat ttgttatcag aatagaaaat gctttatttt tagcttcaat aatattctta
     5041 ggtaatataa ttccctattt tggtcctttt attggtgcaa tccctccaat agccatgact
     5101 atattatata gtccacaaaa aactatatgg gtcattgcat ttctatttat attgcaacaa
     5161 ttagatggaa attttataga accaaaagtt atgggtaatc aagttggtat aggagctata
     5221 tgggtaatat ctgctatttt ggtgggacaa tctctatttg gtttcatagg agtttttctt
     5281 tcagtaccaa tagctgctgt tgtaaaaact tatgttgata aatatataga taatcgttta
     5341 caatagctta tctctactgt gactttaatt tttagtttaa aatttaactg ttatttatta
     5401 aattatttct caaaaaatat ctcttagtaa gaaaaaattg ctaagagata ttttttataa
     5461 tatataattt gttcattaaa tttatgcata tattttatac ttattgatta tttgagtttt
     5521 gagaatttcc accactatct gaaccaccag tagtatcttg tggagttggt gctggtgttg
     5581 gattactatt atttggcttt tcttgatttt gagaagaact gttattattg tcagacttat
     5641 tactatctga ttttttatca ttatttttag aactatattt attattttta ctacttccac
     5701 tataagaaga tttccttgca tctgattcaa ctctatacct atcttctcca gccgtatctt
     5761 ttgccttttt ctttctttgt tcctctgctt cgttttgttc tttttctaat ttagttaact
     5821 cttctttctt tttagttgcc tcttctttaa taacagagga accatttttg attttctcta
     5881 taagtttaag ttccttgatt gcaccctcat aattttttaa ttcttcaaac tccgtcatat
     5941 ttgacattct catagcttgc atatacatag ctcttgcatt ctcattagta ctatcttctt
     6001 ccagtgcaga ggataatgat tccatagctt ttttatagtt atgtttttct aaatatgttt
     6061 ttccttgctc tactaaattt atttcatctt ttttattaaa tgaacatcct acaagaaata
     6121 tagataatat cgatattaat aaaattactc ttcctctcat taaatccctc cttgtcatat
     6181 actttaaatt ttatcataat tataaagttt ttaaaatatt ttatatttat tacaagaaat
     6241 ttgttagtaa aacaaaaaat aagcagatat gctacgaacg atacaataaa ccaaaaatta
     6301 atttagagat acatatccta gcaaatctgc tcataataaa ctttaatatt taatttttat
     6361 tatatacttt tccattttat atctctaaag ctaataagtc ctcatagctt tgtctcttac
     6421 aaattatctt atcatttcca tcaaatactg atactactgc tgctcttggt attttattat
     6481 aatttgaaga cattgaatat ccatatgcac cagttgatgt tgttataagt atatctccac
     6541 ttttaatatc cattatgttt atatctccaa ttaatatatc tccactctca caacattttc
     6601 cagctattgt tacatgattc atagcctcat gattaatttt attcacaata ctacactcat
     6661 aactagcttg gtacaacgaa ggtcttacat tatctgtcat acctccatct acacttacat
     6721 aaaccctaac atcttttata tcttttatag aacctactgt atacaatgta cttcctgcat
     6781 ttgcaactat tgatcttcct ggttctatta ccaatgtagg tacttctatt ccaagctctt
     6841 tacatgaagt ttctgcttta gtgattatgg tttcacaaaa ctcttttata gttttaggtt
     6901 tatctccttc gctataataa acgccaactc ctccacctaa gtcaatttct gttagttgta
     6961 tgttgaattt ttcttttatt tcttttacca agttcatcat tatatccact gtatctatgt
     7021 atggttctac atcaaatatt tgtgaaccta tatgagcgtg aagacctaca agtttaatat
     7081 ttttatattc acttaatttt tctattgctc taaataaatc tccatttact aaagcaaatc
     7141 caaatttaga atctatctgt ccagttttta tataatcatg tgtatgagct tctataccag
     7201 gagttattct aaagtatatc tcttgagttt tacctttatc ttcacataat gcttcaagca
     7261 aatctaattc ataaaaatta tccactacaa atttacctac acctaaatct aatcccatgt
     7321 ttatttcttc tttagtctta ttgtttccat gaaagaaaac tttttccata ggaaaatttg
     7381 atttataagc tgtatataac tcgcctcctg aaactacatc taaacacatt tgttcttcat
     7441 ttataagatt acacatgtaa agaggtaaaa aagctttccc tgcataagca accttatttt
     7501 ttccctctct agccttaaaa tattctatat attctctaca attttgtcta actaaagctt
     7561 catcaaatac ataaagtgga gtttgatatt ttttactaag ttctaaacaa ctcactcctc
     7621 caatatataa ttcatcatta tttacagtca tagttccatg aagtttcata atagcctccc
     7681 caaattttat atattaaata cttctgctat tttttctata gctacttgct tatcatcaga
     7741 atttatagca caagttatac gaatctctga tgtagttata agttttatca ttataccatt
     7801 ttcattgaat atcttaaata ctttagcagc aactccagaa gtatttttca taccaagacc
     7861 tactaaagaa aatttagtga tattattatc aatcactaca tgttcttcat ctgtatattt
     7921 agatactatc tttttacatt ctcccaactc ttcttttgga actgtaaatg atacacttat
     7981 cttatctaaa ataggtgcag tttggcttat catatctaca cttataccta tagttgctat
     8041 atcttcaaat aagctagata tattttcagc tttaaaatct tttattgtta tagaactgtc
     8101 atcatcacta gtagctaatc ctgttattac tttatcctct aatttcatgt cttttccccc
     8161 tcttatatat gttccttttt ctgtaccaca tgtacgtccg acatagattt caacaccata
     8221 tttttgtgca agctctatac ttcttgaatg cattacttgt gctcctaaac tcgctaactc
     8281 aagcatttct tcatattcaa tctcatcaag tttactagcc tttgaatatt ttcttgggtc
     8341 tgtaaaataa attccatcaa catctgtata tatctcacat tttccattta atttaaccgc
     8401 aagagctaca gcagacgtat ctgaaccacc tcttccaaga gtggttacat caccatcttc
     8461 gttgattccc tgaaatccag ttactatcac gacatttcct tcatctaaac tatttttaat
     8521 tctgtttacg tttatatcat caatttgaga ctttccatga agaccactcg tttttatatt
     8581 taactgataa gcattataac ttatagcttt acatccaagt gcatttaatg ctattgataa
     8641 taaagatgct gaaatcattt caccagtaga cattaaagca tctaattccc tcttactagg
     8701 ttcattactc aattcctttg ctaaagttat atattcatca gtggattttc ccatggcaga
     8761 cacaactatt accatttgtg gattttcttt tcttcgttct attatatttt ctgctattga
     8821 ttttattttt tcagtatctg ctactgaact tccaccatat ttttgaacaa ttatactcat
     8881 gctttctccc cctcaagcta aattttgata aataaaaacg cccaaaggta tactttggac
     8941 gtgtaaattt catagaccaa gtaaccttgt agctcaccac tttaaaagtg acagccttat
     9001 gcatattctg cataagtcca gaaaatttgt ccgtcaacaa aatttcttcg gctatatacc
     9061 ctttcatcaa ttatcaatgc ttaccagctc ctattgaata ctattgaata cagctacctc
     9121 taccctaggc tcattatttt taattaattt attaatatag ttttatcata ttctatataa
     9181 tattgcaaca attattttac aaaatgtggc aaatcgatag ttttcttttc taaagttagc
     9241 ttatatacaa ttaaatagtt tatttaatcc taaatctata aaatttatgc tatactcatt
     9301 atcatatctc tctaaatcat ttaattcgga tagattatct ggtgtaacta ccaaaacgtt
     9361 tggtacttta aaaaatggaa ttgtagattt atctattttt ttagaaagat tttcgtattt
     9421 agaaatgtct atttcattat ttaagtcaac atcaactaaa acttgcttta tattaccaaa
     9481 ttggtctgta tattcaataa ataaatcgta tttcacatca tctacactaa catttcggta
     9541 aaatcttttt acatcacaac ctgcagtatt gattctaaca actaattctg atcctactag
     9601 agcctttatt aaatcatctc ccttaaaaag tttactaccg tttaaataat atacataaga
     9661 gaggtcttta taattataat aatccacatc acaaggatat ttttttagtg tatattcact
     9721 acacattttt ctcaaagttt ttttaaaatt ttttttattg tatctttttt ggtctccata
     9781 aatttttcta aattgagact ctgtaacaca tctaaccctg ctaattaaaa gtaatatctc
     9841 tttttccata ttcgtcatag tacattctcc ttgaagtatt atttgcaaag ttgtactaat
     9901 attatattaa ttttatgaca ttaaatttac aaagaattaa aataaaatag ctaattttta
     9961 tagaattaaa ttctataaaa attagctatc actatgttaa atactattat caatcttttt
    10021 atttaaatta tacattacct tgattttcag aatctttgga atctgtattt tcatcagttc
    10081 ctgttttttc ttttgctttt ttatcttcaa agtttgcaat atttaggtta tcaaattctg
    10141 acatttctat tacaccagat tcttcttttg aatacgatat tttgacttta tcaccaacct
    10201 gagtcaatgg caattcgcta gatacttttg aagtagccct aaatattata tctttatttg
    10261 aatctaaagt tacataataa ttagtgttac cacctcgcac atctgggctt attcttgtca
    10321 ctgtgccttc taatacctca tcttttaaat cattatcaag ttttaaatca ttacccttag
    10381 attctaatgc atccttatag tttcttaatg cttcattctt atcttcacct attccaagta
    10441 cattataatc ttcaacagat acaaatgcaa ctctcttaac aagaccagcc ttatctttta
    10501 atgatgatac atatgttggc tgacccaata tattatacat tacagggaac gtcgcttcat
    10561 aatttttttc ttgaacatta ccttcagctg atttcattgc agccgtttca gtagctcctg
    10621 gttgtttata taatctagct tcttttgttc tagaatcaac taacataaat ccaattgtag
    10681 attcatctcc acctgaagat gttatacctg tataccaata agacctgtta tcattgccat
    10741 aaactaatga tgtaccttct gttggtacta aaactccctt ttctgatata acagagttta
    10801 aaaatccatt aacataaaca ccccagtctt taatctggtc tgttacaaag ctttgtggtt
    10861 gtattctatc cacccattta ggtgtgtttt taacatcata aacatttatt tttccagttt
    10921 ctgcatcaac agtggctatt cctgttgcat ttgctcctcc atagccaatt ttatgttcat
    10981 ataaacttac aacccaataa ggtcttcctt catcatttat ttcaagagtg aaatctgtca
    11041 taccaacatt tacaatacca tgtatgtata aatgtctttg taaatcctga tgtaagtagg
    11101 cttctggttg gtaaactatc tttattggct ttccatctat ttcttgaact aattggacgt
    11161 cttgaggatt actagctgat acttttacat aaccacttgt accacttaga gatgttatcc
    11221 atttaattat gtctctgtga acaagaggcg ctacccagta caactctcca tctacatttt
    11281 gtatatgaaa ttttcctaat ttggatacac tccctattgc aggaacttct ccaagctttt
    11341 tgtcacctaa tttcattgcc atatcttcat ctacaagacg aatgtcattt acactgacag
    11401 ggctaatgtc atcagtaaat ttactttcag ttacttttcc caatagttct ctatatgatt
    11461 ttgcatgaag tacaggtgta gaagttataa agggaactac tacaatatac acaagcaaag
    11521 ctattgctat tgaaaaatta tatttagcca ttttagatgc tctttcattt ctatctaaca
    11581 tcatatctag tattcctgct acaacaaaga aaattattcc tactgaaaat aaagatgtaa
    11641 aatctaatct tagtacaggt aatttaataa atgcataaat tattacaatt attaatgcaa
    11701 ctccatatgt tttaagatat cgtttcattt aatactcctc tctaaaaaat gttattatta
    11761 ttattatatc catatattta caaaaaataa atatttatca tatttattat atattaagat
    11821 atctttatat atttatagtt ctgcttattc aactatattt aacattatac gtgtatgtat
    11881 aaatactttt tatacactgt ttgtgctatc catcccgcac ctaaaggagt gggatttttg
    11941 cactattctg tattatatat atccaaaaat attataaaaa tacaaatatt agcagaaatt
    12001 taaaaactga agaaagtatt ctaactaaga tttacaaatt cttaatccta tcgaagttaa
    12061 actactatct tcagcctaat tatctagcaa tattttttta tatgaaaaac tctaaatcaa
    12121 gtttttaaat gtttgcgatt tttgatacat aagaatgttg tctagaatta tattctgata
    12181 ataaaccttc atactctaag gttaagccag ataaattata aaaatcattt ggcgttactt
    12241 caaatgcttg tttatttaaa atgtctatct cacttttttc taacatgtca gcaacaaagt
    12301 ttgaacatac atatttatat tcagattgtc tctgaatatt cattagtaaa tatattaatg
    12361 ccattgtatc gtaatagtaa tcttctctat aatcactaat taattttata tttttatata
    12421 gattttcata ttgtaaatta tctacttcta acgaatatac cctacacaca gtgttttttc
    12481 ttatagcata caatccttga tttatatttt cttcaacaaa tcctccaata aatggattcc
    12541 ttggatttag ccttccaaaa gaatacattg gttttagatt ttcatttaat gatattgaca
    12601 catgtgtata cagcttttta cttatattcc ttattaagta agataatata gttcctgtat
    12661 atgttgttac aatatagact ttattcatca tttcccccca atctagtaac taaatgatat
    12721 tatacactta caatttaata tcataagata ttctaacata ataaatgtgg ttttacaaat
    12781 tttgatttta aggtacttta atattatata atataataaa aatataaata taagttttct
    12841 atatttttaa ccaacagatg ttttcttcaa attaatcaga tgatttagta tatcttgaac
    12901 ctttccagag tcctttaaat atcctaatgc cccatttatt tgtgatttta aatctatttc
    12961 tacttccata tccaaatcaa catttccttt ttttatagat atatttttaa gtgttattgg
    13021 gtaaagataa cttttttcca aagtaatctt atttccatta acctcaaatg gaactttttt
    13081 attgtagtta cttaatattt tttctagcat tttatcactt agtttaaatt ttccaatttt
    13141 cacattctca agacttacaa ctaagtttcc atcctttaaa gtaggtctca attcaagttc
    13201 atattgacta tttaccaaac caaatacctt atatggtcct gctaccttta ttttaccatt
    13261 tttcatttca acaaaattat tttcaagttc attaattcca tgcttctgca tcaatgtata
    13321 tattaaattt ttaaactctt cctctgaaac agatattttt ccaaccattc taagtggatt
    13381 ttttactacc tccatagaat ttatatatga gccttttgat aaatattctt catctatgat
    13441 tttattattt tgtgaactaa catcatatct ttcttttggt gtcaagatat atacaacaat
    13501 tcctgcaagt actaaaacaa taacaattaa acttaacaaa atctttgcaa cctttcccat
    13561 aatcgtctcc tttacaaatc tgtcatcaga tttaaaataa atttagataa ctaacttcac
    13621 ataatattct taacaatata catactataa tacagtcttt taaaaattaa ttcaaccaat
    13681 ctaatgtttt gtaaataaac tgtaaaaaca tgtttttagt ataaaagtta atattttgca
    13741 taatactctt aattacacct ttattttttt ctaatcttgg attaaaatta atattatgca
    13801 atacagtgta gtgttctgta ttataatcaa attaaatagg aggtatagaa tatgtcaaat
    13861 gaaacgaact catctaactt tataaagaat attattataa acgacttaga aacaggaaaa
    13921 catgacagta tcataactcg ttttccacct gagccaaacg gatatttaca tattggtcat
    13981 gcaaaaagta tatgtcttaa ttttggatta gctaaggagt ttaatggaaa agctaattta
    14041 agatttgatg atacaaaccc attaaaagag gatgttgaat atgtagagtc cataaaagaa
    14101 gatgtaaagt ggttaggatt tgattggaac gagttaaatt ttgcatctaa ctactttgat
    14161 gaaatgtata agagagcttt aatactaata aaaaagggta aagcttatgt atgtgatttg
    14221 acacaagagg aaatgagaga atatcgtggt actctaactg aaccaggaaa agaaagtcct
    14281 catagaaaca gaactattga agaaaacctt gatttatttg aaagaatgaa aaatggtgaa
    14341 tttaaagatg gtgaaaaaac attaagagcc aaaatagata tgtcttctcc aaatataaac
    14401 cttagagacc caattatata tagaatatct cattcaacac accataatac aggagataaa
    14461 tggtgcatat atcctatgta tgcttttgct catccaattg aagatgcaat tgaaggaata
    14521 actcactcta tatgtacttt agagtttgaa gaccaaagac ctctttatga ttggtttgtt
    14581 aaagaatgtg agatggaaaa tatacctaga caaatagaat ttgctagact taatataaat
    14641 aatactgtca tgagtaaaag gaaattaaaa caacttgtag atgagggtat tgttgatggt
    14701 tgggatgacc ctcgtgttcc tacaatatcc ggaatcagac gtaagggata tactgcagaa
    14761 gcattgcgca atttctgtag tgaaataggg gtatcaaaag ttaactctac ggtagatagt
    14821 caaatgcttg attatttcct aagagaaaac ttacaaccaa aggctcctct tactatggga
    14881 gttttaagac ctttaaaact tattataact aattacccag aagataagat tgagatgctt
    14941 gaaatagaga ataatgctaa agatgaatct caaggaaaga gattagttcc tttctctaga
    15001 gaattgtata tagaacaaga tgattttatg gaagaacctg ttaagaaata ttttagattt
    15061 ttcccaggaa atgaagtccg tttaaaaggt gcttattttg taaaatgtac tgatgtaatt
    15121 aaggatgaaa atggaaatgt agttgaaata catggtactt atgaccctga aactaagagt
    15181 ggctctggct ttacaggacg taaggtaaaa tctacaatac attgggttga tgctaaatca
    15241 gctataccat gtgaatttag attatttgaa ccattaattc ttgatgatat acctgaaaat
    15301 gaaggtaaac atttcttaga acaaataaat cctaattcat tggaaatatt acaaggtttt
    15361 gttgaaccta cacagataaa agattctaaa ccttttgata aattccaatt tgttagaaat
    15421 ggattcttta gtatagataa taaatataca actgacgaaa aatttgtatt taatagaata
    15481 gttcctctaa aaagttcttt taaaccaggt aaataatctt acaatcaaaa aatgaagact
    15541 taaaatataa attacttcta ctataaataa acttagttag aagtaatttt ttatttgagt
    15601 cttcatcatt tactttaaat gattaacttt tttcactaca gattcccata acttctattc
    15661 catcttttat gcattctatg ttaagaggaa aatctggtcc tttttctccg tcaatatctg
    15721 ttataagtga atcttcacat tctatatgca cctttttagt tttaaagtat agtatttcat
    15781 ttccattata ttgatctaaa taatcacctc taagcacact tatcaaaact ggtattgatt
    15841 ttggtattgg cattccttta aaaataatta catccaaata tccatcatct atttcagcct
    15901 tatatgctag atttatattt cctgctgttt tgccattaaa aatcaacata agatacatat
    15961 ctccatcata atacatttcc tcagaagata cttttatctt aaatttcctc atatacaatg
    16021 cttcttctat tccctttata taataagaaa ttcttccaaa tgaatttttt aagtctgtgt
    16081 ttatcctttg tgatacatct gtaaacatac cagcacttgc aacatttata aagtacttat
    16141 tattgacttt ccctatgtct attttctttg gttttgaatt tatgatttca tttatagctt
    16201 cttttatatc aaatgaaatt tgaagagcat ttgaaaaatc atttgcagtt cctgtaggca
    16261 aaattccaat tggaattttt atgtctaatt ctttcattgc atttactact aaatctacag
    16321 taccatctcc accagctatc aatatgtgtt cataagaact atcaacttcc ttaaatgcat
    16381 tctttacagg ttctttaata gttaatctat agggtatcaa taaataattt ttttcttgat
    16441 ataattctat gatagtgtct aaatttgata atatcttttt ttcacctgaa ttaggattgt
    16501 atataagttt tacttttttc attatttctc cttactcatt ttattctttt agtatccaaa
    16561 atcttcattt aaaaaaatca catgtattct ttctggtgtt aattgtcttt ttataagtgt
    16621 ataactacag catattcttc ctggacttga acagtctata catataccag tagtcttaca
    16681 tccagtattt gtacttaatc ttttacaatt ggctggagca gctattgttt ttgttctttt
    16741 tatagcttca tctacatctt taactatttt atttactcct gctataacta ttactttatc
    16801 aggtccatat atcatagcag caactctatt cccattccca tctaaattgt aaagttctcc
    16861 atcctctgtt atcgcattag tacttgtaaa ataagcatct gcagaaaatg actttctata
    16921 tatttctttc atttcttctg gtttgatatc ttttgcatat ctatcaagaa aattatattt
    16981 ttctcttata aaatcaatca ctcctgtttc aaataaggtc atagaacctc cacatcctac
    17041 tagagaacct actttaacta tttcttcaat ttttttaaga agctcttctt tgttacttac
    17101 taaataccct ttcatattat tgttttccag agattttatt gttttttcta ttcttttttc
    17161 attaagccaa tttaaatttt gatccatttt aacttccccc ttatttttaa tatgtactta
    17221 attatttata aactttattg tctttataag tttattttag tacatctaaa aaaaatacaa
    17281 tataaaactt aaatagtttt atataatttt ttccaaaata acttactcaa tcaaaaaagg
    17341 aaagtagatg tactattcta ctttccctat attgtatgta gtatttattg cctaaaatct
    17401 atatattaat tatatgtttc atattatata atatcacagt taattaaaat gtaatactat
    17461 cttcttttta aacatataaa ttgtattatt aaatttaata tatccaactt caataaatct
    17521 tattaatttg atgaagaaga tgctatagct gcacttgttg ctgccattac acatgcaatt
    17581 tgaatagcaa caacaatatt taaaaatata ttattagtta tagagcctat attattctca
    17641 tttcgtatca aatctgcata aattgaagct acaatagcag aactaatcat tactctatta
    17701 ctttttccta aagaccaatt tccaaagcct ttttcttctt taagtttatt tgaaacattt
    17761 tttatctctt ctattatttc atcttctcta taatctaata atgaaattgc tcctattatt
    17821 ggatatccat aataattcat tttacaatca ttttctttaa atgctttttt aagttcaata
    17881 gtctttttac atttaagcat agttctatca tcactaaata aaagaatttg ggataatgct
    17941 tgtaaatcat ttcctttata aaaaccattt tcatttagaa attcataaca tttttctatg
    18001 tattctaaat cttcatctaa attttttgaa tgaattgcaa taagtgctat attacaatag
    18061 tcatcactag aagttagaaa tggatgtttt ttcctcatgt aatcatatat tattttcatt
    18121 tttgatatac atgtaagata atccattttc tctctatttt cataaactat tgatgctgta
    18181 agtggtaaat atgtatcgcc ccaaaacttt tcacttttaa gttctttgta tatatctaaa
    18241 atcatcttaa aactactttc tggatttgat tcaaacgaaa gtagtgttgc catataaaac
    18301 atattatatc ctctaaaatt agagaataat ccagtatttt ttttaataag ctctattcgt
    18361 tcttccacta atctaggact tattctcttg ttcttaagag taaatgcaag tgcacaacta
    18421 tgttgtatca atcccatttg ccaagaacct ttaacttcat ttaatgcttt gtaattttct
    18481 attaataagt ttacttttcc atttataaac gattgttcca tattgccctc ctagtaatat
    18541 atctcattgt actaatagtt ttatatttat tatatatata ttaaatacta caatcaatac
    18601 atattacttc aaagaacatc attcttcaac aataatgtag ccttttttgt aatgtcttcc
    18661 catattattg actctatcat agtactctga ttcactttca ttactccatt tgggcacaaa
    18721 tacatctatt gcattagaat tactaaaata cttactagac cccctatcca taacagtttt
    18781 taattttcca tctacaataa tcttagttcc aaaactcaag tgattgtttg ctacaaaacc
    18841 atcttctaaa tcatctcctg tagcagttat cgctccatat ccattttctt gattaagact
    18901 tgtgtaataa ctaacttcaa aagttatagg tactaacttc tttattttaa tacctaactg
    18961 attttgtagt ttctcctttt gtactttcaa cttatactca tcactatttt tataatcttc
    19021 cataccttga ttatacttgg aattaaagct tttacctgac tttactatag tatctatatt
    19081 tactatagat tctttacaat gatctgcatc tatttttgtt tctccattta ccaataatgg
    19141 agttaaacat acactacata caacacatgt aatcaatgct tctttaagct tcataaattc
    19201 cccccttatt attttgatat attagcatat ttaatattat atgtctatat ttagatttaa
    19261 ataccacacc catttgcttt aagaagttgt cttatattta attcacctta aacacttata
    19321 aa
//
LOCUS       DACTGW010000057        19136 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.057, whole genome shotgun sequence.
ACCESSION   DACTGW010000057 DACTGW010000000
VERSION     DACTGW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19136
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.057"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            421..3969
                     /locus_tag="I9573_001573"
     CDS             421..3969
                     /locus_tag="I9573_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT4796199.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINNEMVIALMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTISCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLALTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKIIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDNFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESASNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMRNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEVTSIEAEENT"
     gene            4154..6088
                     /locus_tag="I9573_001574"
     CDS             4154..6088
                     /locus_tag="I9573_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAT4796200.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6194..7189
                     /locus_tag="I9573_001575"
     CDS             6194..7189
                     /locus_tag="I9573_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT4796201.1"
                     /translation="MKKYISIILLVVVTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSNIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7161..7946
                     /locus_tag="I9573_001576"
     CDS             7161..7946
                     /locus_tag="I9573_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAT4796202.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7934..8632
                     /locus_tag="I9573_001577"
     CDS             7934..8632
                     /locus_tag="I9573_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796203.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8755..9228
                     /gene="ybaK"
                     /locus_tag="I9573_001578"
     CDS             8755..9228
                     /gene="ybaK"
                     /locus_tag="I9573_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAT4796204.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9425..10264
                     /locus_tag="I9573_001579"
     CDS             9425..10264
                     /locus_tag="I9573_001579"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAT4796205.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10277..10825
                     /locus_tag="I9573_001580"
     CDS             10277..10825
                     /locus_tag="I9573_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HAT4796206.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10891..12072
                     /locus_tag="I9573_001581"
     CDS             10891..12072
                     /locus_tag="I9573_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAT4796207.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYIYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12245..12508
                     /locus_tag="I9573_001582"
     CDS             12245..12508
                     /locus_tag="I9573_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT4796208.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            13034..13717
                     /locus_tag="I9573_001583"
     CDS             13034..13717
                     /locus_tag="I9573_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4796209.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            13728..14969
                     /locus_tag="I9573_001584"
     CDS             13728..14969
                     /locus_tag="I9573_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4796210.1"
                     /translation="MKIFSNKDIKIFFLILISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIVLNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISINGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSTFTISFLKLTNM"
     gene            15041..15724
                     /locus_tag="I9573_001585"
     CDS             15041..15724
                     /locus_tag="I9573_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796211.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            15721..18051
                     /locus_tag="I9573_001586"
     CDS             15721..18051
                     /locus_tag="I9573_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAT4796212.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDSGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANTGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNRNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAMSGCLLGSILGLMLHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPVKKICELDIIDSINAQ"
     gene            complement(18232..19074)
                     /locus_tag="I9573_001587"
     CDS             complement(18232..19074)
                     /locus_tag="I9573_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAT4796213.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKAGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
BASE COUNT         7603 a         1988 c         3369 g         6176 t
ORIGIN      
        1 gtattaatct aaaatatatg tttttatagg gtttaaattt tatgtataca aaattaattt
       61 gctaactgaa tcatataaaa aaaacaaaat attttttaga gggaatgatt agatactatt
      121 ttctttgtta tgaaattaat tgaagagtaa ttaattagaa aaggaataaa taataatgaa
      181 tatattacat tattactttt tgataaatca aaaataaagt tatagttttt aatattaatt
      241 aagaatgatg aaatatgaat aaagatttta caaataaatc ttaagctatc ttatttaatt
      301 ctaatgcagg aagaaggtaa atgtatattg aaaaaatcat agaaatagaa gtaaacaact
      361 taaacaaata caaaggtttg caaaagaagt aatataatta tatacaatga ggatgtgagt
      421 atgatgaaca gactaaagcc aagaatttta ggtattagta tcttgtttat tattataatt
      481 attttaacat gtggagctgt tttcaagttt aaatttaaat tagataataa attaaaagaa
      541 accacacata catatttgtc tgagtttaca gaacacaata taattaatct caagacaaga
      601 cttagtggtc aatttgacat gcttgaatct attgctaact tcataggaaa cttgaataat
      661 ataaataatg aaatggtgat tgctttaatg gattctgatg taaagcgaag ttctttaatt
      721 agaatgacta tctctacatt agatggggaa tgttattcta atgatggaat cacccataat
      781 gttcaagata gagattattt taaaaaagct aaaaatggag aaaagaatat ttcagagcct
      841 ttaaaatcta ttgttaataa taaagaaata atagttttat ctaccccagt ttataaaagt
      901 gggaaaatag ttggtgtttt aagtggaatt tatgattcaa aaaagttaaa tgagttatta
      961 ggattatctg catttgatga aaaagcagat gtttttgttg caaagaaaaa tggagatata
     1021 gttgctcaaa aaacaaataa aaatagaaag acgatatcat gtatatttca gctttttgac
     1081 aaagataatt gtgatgaaga tgattgtcta aaaactataa aagaaaatat gaaaaatgat
     1141 aagtctggat attttaatta taacttagat aatgaaaaat ttatgttaaa ctatcagcca
     1201 cttggagtta atgattggta tgttttttct gttgtgccaa ataaaatagt gtctattcag
     1261 tcagagaata tatcttatga tgtatatatt tttgcattag aaatactact agttatattg
     1321 atttttataa tatatatagc atgtattata aataataaca ccaaaattat tataaatgaa
     1381 aaagaaaaat ttaaagtact cactgataat atattaggag gggttaaaac ctgtttaaaa
     1441 gatgaatata tgactatgac atatgtaagt caggggtttt tagcgcttac aggttactct
     1501 tgtgaagatt taaataaaat atttaaaaat agatttaaag acatgattta taaagatgat
     1561 ttagacaagt ttaattgtat tattctaaat caattagaaa aacaagataa aattgaagtt
     1621 gaatatagaa ttgtaagaaa agatggaagt ataatttgga ttttggagag aagtaagtct
     1681 ataaatgata ataaaggaag tgagtgtatt cagggagttt taactgatat aacagaactt
     1741 aagaaaatac aagaagagtt agattcaaaa agatttgaaa tacaggctat caatgatagt
     1801 attgttggtg gaatagtgat aactgagata aatgatgatt ttgatagtat atatttaaat
     1861 gaaggttatt taaatatgat tggatataca cgcaaacaac tggaagatga gctaaataat
     1921 aaattaatca aacttatttt taaagatgat agaacagaat taattaatag tataaacaaa
     1981 caactcaaat taagtgatac atttgcatct gaacaaagag ttgaaaaaag ggatggtact
     2041 attatatgga ttttattaaa aggaagaaag ataatttatg ataatggaaa atctgtactt
     2101 tgttgtgtta ttgtagatat cacttctgct aaattattag agcaagaact taaaaagagt
     2161 gaagaacgtc atgaaatcat tctaaagcag acgcaaaata tcataataga atgggatata
     2221 gaaaaagata aaatgaatat atcacctaat tggtataata agtttaaata tgaccctata
     2281 actgaaaacg caacaaaaaa tataattaaa tcagtagata tatatcatga tgatatggat
     2341 aagtttattt cattaattaa acttacttca caaggtagtg agtatgaaga gtgtgaaatt
     2401 agacttagaa ctagagatac tatttacgct tggtttagta taaaggcaac aactttattt
     2461 gataataatg gtaaacctta taaagtagta ggaagtttga ctgatattga tgaatcaaaa
     2521 cgagaagctg aaaagttgaa agaaagggca gaaaaagacc ctcttacaga tttgtataat
     2581 aaaaaagcat cacaatcact tatagaagag tacataacat tggataaaaa aagaaaaggt
     2641 gctttaatga ttattgatat tgataacttt aaaggaataa atgataattt aggacattta
     2701 tatggggatg ctgtattaag tgaaattgca aatgacctaa taaatctatt tagagcaacc
     2761 gatatagtag gcagaattgg tggagatgag tttattgtat ttataaaaga tatttctgaa
     2821 acaagagata ttataaataa agcagaagaa ttggtaaaaa catttgaaag gtctttttta
     2881 ggtaaaggac gaaattataa aatatcatta agtgtaggaa ttgctaagtt tccacaagat
     2941 ggtatggact atatgacact atttagaaat gcggatatgg ctctttactc agccaaaggg
     3001 aagggtaaga attgttatac attgtatgat aaaaaaattg ataaagtcca atatataact
     3061 aatacaagaa atgcaggaga tagtgataat gtagagatgt cttttaaaga acatatcaca
     3121 aattatatat ttgatatact atatgaatca agtgatatag aagtggcagt aaatttaatt
     3181 ctaagtattg taggtaaaca ctttaatgtg agtcgcatgt atatatttga aaattcagat
     3241 gataatcgag tatgtaaaaa tacatttgaa tggtgtaacg aaggtgtaac ctcggaaatt
     3301 aataatctta aaaatatttg ttacaaggat ttaggcaact atcttttaaa ttttaatgca
     3361 gatgggttat tttattgtaa tgatattgaa aatttaggta gtgatgtgaa aaaagtactt
     3421 aagccacaag gcatacgttc aatattacaa tgtaaaattt tagagggcaa tgaatttaga
     3481 gggttcatag gatttgatga atgttcatat aatcgtatgt ggactaatga tgaagtgaac
     3541 actttgacat ttatatctaa aacgctcagt atgtttttat taaaaatgcg tattcagagt
     3601 aagctagaag agtctttaac aattcaaaaa tctgtgatgg ataatatgga tatatgggca
     3661 tatgttgttg acaggaatac ctatgaatta ttatttatca ataaaaaaat gcttgagtca
     3721 gcatcaaaca ctcatgtagg tgatttatgt tataaagcga tatggaataa tcaagataaa
     3781 ccttgtaaaa aatgccctat gatatattta aaagatggtg atgatactta taaatcattt
     3841 atgcgcaata aaaagtttga ggtacaggca aatataacta caactacttt gaaatggttt
     3901 gatgggaaag atgcatgttt aatatttgga agtgaagtaa ctagtattga agctgaagaa
     3961 aatacttaaa atactgtgtt atatatatac ttaaaatcgc aatttgaatt tattagactt
     4021 aaaatgtcta tataaatcat ttaagttgtg gttttaagta ttttttatga tataattaag
     4081 cgttaagaac aaaattaaaa gatacttttg tataaaaaaa tatttgttaa atatatattg
     4141 agcaggtgat aatatggaaa ataagttttt accaataagc aaacaagata tgatagatag
     4201 aggatgggaa gaattagatt ttgttctagt tacaggagat gcttatgtgg accatcatag
     4261 ttttggaaca gcaataatat ctagagtttt agaaaatgct gggtataaag ttggaataat
     4321 agctcaacca gactggagaa ctacagatga ttttatgaag ctaggaaaac cgagacttgc
     4381 atttctagtt aatgcaggaa atatggattc aatggtcaat cattattctg taagtaaaaa
     4441 gcatagagaa aaggatatgt attctccagg tgggaaaatg ggttatagac cagatagagc
     4501 tactatagta tattgcaata aaataagaga agcatacagt gatgttgcaa tagtaatagg
     4561 tggaattgaa gcgagtttaa gaagatttgc acactatgat tattggagtg ataaggttag
     4621 aaagtctatg ttaatagata gtggagcaga tttattagtt tatggtatga gtgaaaagca
     4681 gattgtagaa gtagccaatg cacttaatga tggatatgac ccaaaatata ttagacatat
     4741 tgatgggaca tgctatattt cagatacatt ggaagaaata tatgataaat atatattaat
     4801 tccatcatat aaagaaattt gtgaagataa aatgaagtat gtagaagctt ttaaaattca
     4861 atatgatgag caagacccat ttagaggtaa tataattgtt caacctcatg gaagtaagta
     4921 tctggttcaa aataagccag aaaaaccact tagtagagaa gaactagatg aagtatatgg
     4981 attaccatat cagaagacat accatcctgt gtatgaaaaa tttggaggaa taccagctat
     5041 agaagaagta aaatttagta tagtaagttc aagaggctgt tttggtagtt gttcattttg
     5101 tgcaataaca tttcatcaag gtagagcagt gcaaagtaga agtgaaaaat caattattga
     5161 tgaagcaata ggaataacaa atttagatga ttttaaaggg tatatacatg atgttggagg
     5221 tccaacagct aattttagaa gaccagcatg taaaaaacaa attactaaag gtgcttgtaa
     5281 aaatagacaa tgcttatctc catcaccatg taaaaattta gatgcagacc acagtgaata
     5341 cttacattta cttagagctg taagaaaatt acctaaaata aaaaaggtgt ttgtgcgttc
     5401 tggaattaga tacgattatg taatggcaga taagaacaat aaatttctta gagagttgat
     5461 agagcatcat gtaagtggtc agttaaaggt agccccagaa catatatcag aagaagtttt
     5521 aaagtatatg caaaaaccag caggaaaaac ttatgataaa tttagacaga agttttttgc
     5581 tataaatgaa gaattaggta aaaagcaata tttgataccc tatttaatgt ctagtcaccc
     5641 tggttctacc ttaaatagcg ctatagagtt agcagagtat ttaagagata ctcactacca
     5701 accagagcag gttcaggatt tttatccaac tccagggact ctatctacaa ccatgtttta
     5761 tacaggtatt gacccgctta caatgaagcc agtttatgtt cctaaatcaa aacgtgacaa
     5821 ggcaatgcaa agggctttat tacaatatag agcacctaga aattatgatt tggtatactc
     5881 tgcgttagtt gaagcaggaa gagaagactt aattggtttt ggacatagat gtcttataaa
     5941 gccaaaaaat gaaaagcctt attttaatag aaataattct aaaaaaaatg tatctaaagg
     6001 aacaaataaa aacaaaaaaa ctaatacaaa taacagaagt aataaaaatc aacaaaaaag
     6061 ttcaactaaa aaaaagaaaa aacattaaag tataaataaa aatacttaaa aaagaaggat
     6121 tattcgataa attaacagaa attagtataa ataaatataa ttattttaat gacgttacct
     6181 ttgggaggaa gagatgaaaa agtatatttc tataatatta ttagtggtgg tgactatggt
     6241 tgttttggta ggatgttcac caggaaaaga taaccctaaa aataaggagc tatcagttgt
     6301 caaacaagta aaagtagctg ttccagatgg actaccagca gtggcaatag ctaaacttgc
     6361 aaatgaaaat ccagagatta aagaaggata tgaaacagtg tatagcattg aaaagacacc
     6421 tgaggcgatt tccactagag tgatgaaaaa agatgcagat atagctatag ttccttcaaa
     6481 catggctgca atagcgtata ataaaacttc atcatataat attgttggaa ctgtaggaat
     6541 gggttcactt tatttggtgt caacagaaaa tataaaagat tattctgatt tagttggtaa
     6601 agaagttgga tgtactggaa aaggacttac tccagacata actataaaaa gtcttttgtt
     6661 acaaaaggat ataaattatt caaatataaa atttaattat gtaaattcag caagtgaact
     6721 ggtaccatta ttggctactg gtaaagttaa gacaggtatt gttccagaac ctgcacttag
     6781 tgctttgatg tctaaaaatc cagacataaa aattataaaa agtttaaatg actcatggaa
     6841 ggaagtaagt ggttcaaaag acggttatcc tcaatctact ttaattgtaa agtctgaatt
     6901 tttaagagat aataaagatt ttgttgattc ttttgtaggt caattatcaa atagtataga
     6961 ttgggcaaac aaaaatcctg aagaattagg tgcatattct gaaaaaataa aaatatctac
     7021 tgaatcaaag ataataggaa aatctcttga aagagctaat ttaaaatata ttccagttaa
     7081 ggacatgatt aaagattata aaaattacta tgagaaatta gctaattttg atgataagac
     7141 attaggaggc aaagtaccag atgaagcaat atactttgtt gagaaataaa aaaaataaag
     7201 aacaatttat agataaaatg gaggtaacac tttcttgtgt tatcctctta tttttatggc
     7261 aaattattgc tttaaaaatt gataatgata tttttttacc aacagtaggt caagtattta
     7321 atagtataaa agagattatg ctaaattcta gtttctatat agatatactt tactctatgg
     7381 ggagatgttt ttttagtttt ttactggcta tgatttttgc cattatactt tcaatagtat
     7441 cttatttaaa tagatttttt agaaacttat taaagccaat caatgctttg acacgttcaa
     7501 taccaactat gatattggta gtacttgcac ttatttggtt tgaaaaggat agtacccctt
     7561 ttatagttgg atttactata gtttttccta tattgtatga taacgttttg ggcgctatat
     7621 tgaatataga caagaactta ttagaaatgg ctaatgtgta taaaataagg tttatagata
     7681 aggtgttaaa aatttattta ccagccataa aatttcaaat tatatctata ctagtatcaa
     7741 cattctcttt aagtttgaaa gttgctatag caggagaggt atatagtcaa cctacatatg
     7801 gtattggaac tatgatacag actgaaaaaa ttaactttaa tacatctgga atatttgcat
     7861 ggataattat tgttctttta atatctgcta ttttacaaat agcccaaaag tttttagcaa
     7921 ggagagcttt tttatggaaa agataaagtt gagtattgaa aatataaata aaagatatga
     7981 tagtcgtatt atatttagag attttaatat tgatttttat gtaaatgagg taaattgtat
     8041 ccttggaaaa tctggttgtg gaaaaactac actcttgaac ataataagtg ggattattaa
     8101 aaatgacacc aacaatctaa atatcaaaga aaatttaaat agagtaggaa ataagcttga
     8161 agctagttac atatttcaag atgatagact tatagactgg ttaacagtag aagaaaatat
     8221 aaagatagtt gtaaataaat actataataa aactcaatta aataaaattt gtgatgaata
     8281 cttggaattg gttggaatta gtgattataa aaaattctat cctcagatgc ttagtggagg
     8341 tattagacaa agagtaaata ttgctagagc ttttatatat ccatcaaaaa acattataat
     8401 ggatgaacct tttaaatcta ttgatgcaaa gaatacacag cttataatgg ataatttcag
     8461 aaatatatta agaaaagaaa agagaactgt attatttgta actcataata ttgaggaagc
     8521 attgttttta gcagatagga tatttatttt aggagactct ccaattagaa taaaaaaaat
     8581 attaaaaaat tctaaggaat tggaaaaaaa tgaagttttg aagttgatat agagttataa
     8641 taatactgtc gttttaaaat atatttggtt aaaatctata agactatatt aaaatagtac
     8701 agaatattca aaactgtgta taatgttcgt attttataaa aaggaagtga atgtatggtt
     8761 aagactaatg ctatgagaat attagattca aataagattg attataaagt aatgtcttat
     8821 gaagtgaaaa gtgaacatgt ggatggtgtt gaggtggcac atgatatagg aagagatgta
     8881 aatgaagtgt ataagacatt agtaactcaa ggagttagta agaatatata tgtatatgta
     8941 ataccagtac atgaaaactt agacttaaaa aaggctgcaa aagtagctaa agagaaaagt
     9001 gtagagatga ttcatgtaaa agatataaat aagcttacag gttatattag aggcggatgt
     9061 tctcctatag gtatgaaaaa gctttataaa acttttgtaa atgaaagtgc aaagaattta
     9121 gatacaatta ttgtgagtgc aggaaaaata ggatatcaaa tagaactatc tccatttgat
     9181 ttacaaagac ttataaaagt agaatttgta gatgtaataa aaaaataaga attacatata
     9241 aaatatagag cttatattct taataatagg tgttgtaata ttaataaaat gaataataat
     9301 tttaaaataa atattcagtt acttatgtgt aattggtata aaaatattga gaaattttaa
     9361 aaacaatggt aaagtgtaag tataatcacg aatatttttt ataaagtaat ggagggatgt
     9421 tcgcttgaga aaaataaaat ctgaacaaat cgtcgagcag gtaaaaaaac tttgtataga
     9481 agcgagccta tatttaggag aagatgtttt aagttgtata aaagaaaaag cgaaaagcga
     9541 aaagagtgaa gttggaaaaa atatattgaa tatattagtt gaaaatgctg aaatagctaa
     9601 agaaaaaaat ataccaatat gccaagatac tggtatggct gtattttttg ttgaaatagg
     9661 acaagaagtt ttaattgaag gagacacact tactgatgct ataaatgagg gagtaagaca
     9721 agggtatgaa gaaggctact taagaaaatc tgttgtaagt ccaattaaca gagtaaatac
     9781 aaaagataat acacctgctg ttattcatta tgatatggta aaaggtgata aaattaaaat
     9841 agaatttgca gctaagggat ttggaagcga aaatatgagt aagatgaaaa tgttaaagcc
     9901 atcagatgga ttggaaggaa ttaaaaaatt cataatagat acagtttcag aagctggacc
     9961 taacccatgc cctcctatgg taattggagt tggtataggt gggactgtgg acaaatgtgc
    10021 tcaaatagct aaaaaggcac tttttagaga actaggtgaa tttaataagg acgaaaatat
    10081 agcaaaactt gaaagtgaac tattaacagc tattaataaa cttggtatag gacctcaagg
    10141 cttaggtggt actacaacag ctttgggatt aaacatagag acttttccta cacacatagc
    10201 aggtctacct gttgttgtaa atataaactg ccatgcatca agacataaga aggtagtaat
    10261 ttaggaggaa aataaaatga taaaaataac tacacctgta aatgaaattg atatagcaaa
    10321 attaaattgt ggagatacaa ttagtttgag tggtatactt tatacagcaa gagatgctgc
    10381 tcataaaaga ttgatagatt gtataaataa aggtgaagaa ttaccatttg acgtatatgg
    10441 tcaaggaata tattatgtag gacctactcc tacaaagcca ggagaagtta taggtgctgc
    10501 aggtccaacg acgagttata gaatggatga tttgactata cctcttttag aaagaggtct
    10561 tagattgatg ataggcaagg gaaaaagaag tgatgaagtt atagaaggta tgcaaaaata
    10621 cggagcagtt tatcttgctg caataggagg agctggagcg tatatttcaa attcaataaa
    10681 atcatgtgaa attattgcat atgaagattt aggagctgaa gctataagaa agattgtagt
    10741 agaagacctt aaacttacag tagcaataga tagctatgga aataatatat atgagcaagg
    10801 tcgagcaatt tatgaatgta aataatgttt agaaataaat atgaatatca aaattaaaaa
    10861 tagttgtgta tattttaagg aggaaaattg atgtctaagg attatgcaaa attggcatta
    10921 gaaatgcatg aaatcaacaa agggaaagta agtgtagaaa gcaaggtaga agttaaaact
    10981 aaggatgatt tatcaacagc atatacacca ggggttgctg aaccttgttt aaaaatacat
    11041 gaaaatcaag atgatgttta tatatacact tctaaaggga atctagtagc agtagttagt
    11101 gatggaagtg ctgtacttgg attaggtaac attggtgctg aagcatctat acctgttatg
    11161 gaaggtaaag ctatattatt taaacaattt gcagatgtag acgcttttcc aatatgttta
    11221 aagactaatg atgtagatga gatagttaaa tctgtagagt tgatggagcc tgtatttgga
    11281 ggtataaatt tggaagacat atcttctcca agatgttttg agatagaaga gagattaaaa
    11341 aaatctttaa gtatacctgt ttttcatgat gaccaacatg ggacagcaat aatagttgca
    11401 gcagccataa taaactcaat aaagttaatt gaaaataaaa aaatagaaga tttagaaata
    11461 gttataaacg gagcaggagc tgctggtata gccatagcaa agatactact taatatgaat
    11521 gttaaaaata taatcttatg tgatagaagt ggagcattgg aagctagtat agaaaatcta
    11581 aactatgttc aaaaagaaat gttaaaagta acaaatataa gaaatgaaaa aggaccttta
    11641 aaagatatta taaaaggaaa agatgtattt attggtgttt caggtccagg agctgttaca
    11701 aaggaaatgg ttgaaagtat gtctgagaag ccaataatat tggcaatggc aaatcctact
    11761 ccagagataa tgccagaaga agcaaaatta ggtggagcat ttataatggg aacaggtcgt
    11821 tcagacttcc ctaatcaagt caataatgta ctggcttttc ctggaatatt tagaggagca
    11881 ttagatgtta aggctaaaga gataaatgaa gaaatgaaga tagctgctgc atatgcaatt
    11941 gctaatacaa taagtgatga agaaatatgc ccagagtata tattaccaga tgtatttaat
    12001 aagaatgtag taaaaaatgt tgctaaggca gttaaagaag cagcgataaa aactaaagta
    12061 aatagaatat aaatttttat aacttattaa aaactagaaa agtataaaag actagtgaaa
    12121 ttatgtacag tgggatgatt ttattagtct ttttatttta aatatgacac aataatgtca
    12181 tattaagctt gatataattt taatatggtt aggagccaaa gaaaaatata aggagtgatt
    12241 taaaatggaa gaaactaaat tttgccaatg ctgtgcaatg ccaatgggac aaactgatga
    12301 attatatggg acaaataaag atggaagtaa aagtactgat tattgtagtt attgttttaa
    12361 agatggtgag tttacagctg atataagtat ggaagagatg atagaagttt gtatccctca
    12421 tatgcttcaa agtaataagg atatgactga agatgaagca agaacaatga tgaacaattt
    12481 ctttccaaca ttaaaacgct ggaaataatt ttatgcaaaa ttagttatat gcaaaacatg
    12541 aaatatggtg ttgagaataa ggggtggtgt gctttatagc ataccacctg ttttgttact
    12601 tgctagttca gttattcttg tatgcttaaa tatgttctca tctctctttc tattttaaat
    12661 tgtaaaaatc taaaatatgt tatttatatt ctacttcttt atccatgtct ttttctacct
    12721 caagtcaaat acaactacaa aatcatcatt ttcaattcac tgtttaatag aattaacaga
    12781 taaagagtta taaaattttc tattttttat ataaaataat acaggtcaag ctgacttcta
    12841 gctacttggg atagtgtata tatttatgat agttatagga gtaataccaa tcattagact
    12901 gttgatttgt gtagtctttt gtaaaaacat ttgttttaat atattgagtt tttgaaaaag
    12961 taaaattata attataataa attaagtgta ataatgtatt agaagtaaaa ataattaaaa
    13021 tagaggtgat taaatgagca gtatattgtt agttgaagat gatttaagct tgatatatgg
    13081 attagagttc tctatacaaa aaagtggatt tagtatagac atagcaagga cagtaaaaga
    13141 agctatgcaa atgtatgagg aaaaaaatta tgacttattg cttttagatg tatccctacc
    13201 tgatggtaat ggatttcaga tttgcgaaaa agtacgagag gtttctaata tacccattat
    13261 atttttgact gcttcagatg aagaggtaaa tgtagtgatg ggtcttgata tgggtggcga
    13321 cgattatata acaaaacctt ttaaattaaa tgaattaatt tcaagaataa aagcattact
    13381 tagacgttat aatgttgcaa gtaatgtgaa tgaattaagt tcaaataata ttactattaa
    13441 attacttgaa aatcgtgttt ttaaaggtga atttgaggtg gaacttacta cagcagaata
    13501 taaattattg tgcttattta tgaaaaataa aaatattgta cttactagaa aaaacatatt
    13561 ggacaagtta tgggatggaa atggtagctt tatagatgac aatacattat cagtatatgt
    13621 aagacgattg agaaataaaa ttgaagataa tcctgaaaat cctaagtttc tattgaccgt
    13681 tagaagaatg ggttataagt ggaatgtaat taagtgaggg tattaatatg aagatatttt
    13741 ctaataaaga tataaaaata ttttttttaa ttcttatatc tattttagta atgtttatta
    13801 tagtagggca aatagtggtt ataagtataa ctaacaatta taaaaaaacg ttgatagaac
    13861 atgattataa tatagctgga tacttatata gtataggtgt tgataaatct aaaataccat
    13921 cagcatttgt tgcacatgaa gataaatata ttttaaaagg aaaagaaata ctagatacag
    13981 ctggatatgg atttgatata gatgttagct ttacaccaag tgtaaaattg tttcaaagta
    14041 aatatataaa aactatgtct atctatttat tagtattttt tactatgatt attttcatac
    14101 tatatatgta tttttttagg caacaaagta agatagaaga ggctagttat aaaattgatt
    14161 tatttatgaa tggaaatata aatgaaagac taaatacata tgaagaaggt agcttatcaa
    14221 aattatttag ttcaatagat gttatgtcga catctttaaa tacacatata ttaaaagaaa
    14281 aacagagcag agaatttctt aaaaatacaa tttcggatat atcgcatcaa ttaaaaactc
    14341 cacttacagc tttaataatg tacaatcaga tactacaaga agaaagtcat aatagtgatg
    14401 tcgttgaagc ttttgtacaa aaaagtcaaa gtgaactaga acgcattgaa acattgatta
    14461 agaatttact aaagattaca aagatagatt caaaaacaat agttctcaat aaaaataatg
    14521 ttaatcttaa aaatttaatt gtcaaaattt tgtatagctt tgaaactagg gcagaaaaag
    14581 aaggaaaggt tatttctata aatggttcag aaaatacatt gttgttctgc gattatgaat
    14641 ggatgtcaga ggctttaagt aatcttataa agaatgcatt agataataca aaagagaatg
    14701 ataaaataac gatagagtgg tttgagacga ctataaccac cattgtttca gttaaagaca
    14761 ctggaaatgg aatccatgtt gaggatattc atcatatatt taaaaggttt tatagaagta
    14821 agttttcaaa agataatcaa ggtttaggac tgggattacc actagttaaa tctattgtgg
    14881 aacaacataa tggtactatt tctgtagaaa gtaaactatc acaaggaagt acattcacca
    14941 taagtttttt aaagcttact aatatgtaag ataaaataca tcttgcagta aggtatatag
    15001 attattatta tattaatcaa ttagaaaggt gagagagaaa atggatattc ttgtggttaa
    15061 taatttatgt aaaacatatg gaaatggata tacaaaagta gaggcattga aagatgtatc
    15121 ctttacagtt tcaaaaggag agtttgtgac tgtagttgga gaatctggtt caggaaagag
    15181 tacactatta aatctcttag gaggtcttga tactccaaca tcagggagaa tatttattga
    15241 tggtaaagaa atatgttcaa tgaaagagga aaaacagact atttttagac gtcgtaacat
    15301 agggtttata tttcaggcat ataaccttat accagaacta actgtggagc aaaatataat
    15361 attcccattg ttattagatt ataaaaaacc agatactatt tacttagatg aagtattgac
    15421 tattttaggt cttaaagata ggaaaaatca tcttccaaat caattatctg gtggtcaaca
    15481 acagagagtt gcaattggaa gggctttgat tacaaagcca agtattatac tggcagatga
    15541 gccaacggga aatcttgaca gtaaaaatag taatgatgta atttcacttt taaaagtgac
    15601 tgcagaaaag tataatcaaa ccattattat gataactcat aatacgaata tagcttctac
    15661 agcagataga atactacagg tttctgatgg tgtgcttaca gacctggggg tgaatagaga
    15721 atgagaagct acttagaatt agtaccacaa tatgcaaaag tacataagaa aaaaaataga
    15781 actacaattt tatgtattat tattgcagta tgcttagtta ctgctatctt tggacttgca
    15841 gatatggcta tacaatgtca aaagatacaa tttattaaag atgatggaaa ttggcacata
    15901 gtaattagaa atattgatga aaagtttata tcagaaataa atagtagagt agatatagct
    15961 caccatggta gggggcaagt cgttgacagt ggagagttta aatcaaagga gattttgata
    16021 caaggtttag atgaagaagt agcaaaacaa tttaacttag aaataataag gggtcgttat
    16081 cctattgggg agaatgagac tttaatagat gaggatgcag ttaaaagctt taatctaaaa
    16141 ataggagatt ttatcaaaat aaaattagca gatggtagaa ataaaaatta taagatagta
    16201 ggtgaatatt catcaatatc aagcttaaga gctacagata aacatggatt aatactttca
    16261 atattgggta cacaagctat taaaaaatca gaaacgtacg attattatta tattacattt
    16321 aaagaaaagg ttaatattag aaaagccact tctgatatta aaaatacctt caatttaagt
    16381 gataaacaaa tcggtaaaaa tgaaagatta ctttctatta taggtcaagg cgactctgac
    16441 ttttctaaac cactgtattt aacagcagga ttactttttg tacttgtttt agtagcaagt
    16501 attatcatga tatataatag ttttaacatg agtgtaattg agagagttaa attttttggt
    16561 ctgttacgtt gtctagggtc atcaaaacat caagttaaaa aatttgttgt tttagaaagt
    16621 ttaattttaa gtataaaagc tattccaata ggcttagtat taggaagtct tgtaactatt
    16681 atatcttcta tatttcttaa gtatgttaat gagttattca gctcaatgcc aattttaaaa
    16741 gttagtttta ttggtattgc atttggaata attgtagggt ttttaactgt aatattatca
    16801 gctattattc cagcaaaaaa agcttcccgt gtatctccac ttagcgctgt acgtgggaat
    16861 ctaatgtcca aagaaatttt aaataacggg aagacaagta aagttgcaaa tacaggaagt
    16921 tttaaaattg agacttctat gggattaaat catgcttttt caagaaagaa atcttttgta
    16981 cttatgactt cttcatttgc cattagtatt atactatttt taggttttag tgtgcttata
    17041 gaccttatgt atcaaggact taagcctatg aagccagaag ctcctcatat atctattgtt
    17101 gaccctaatt ttaagtctgt tttaactaag gaagatatta atgaaataag caatatcaaa
    17161 ggagtaaaga aagtatacag tagaatgatg acggatatag aagctacata taatgggaag
    17221 aagacaaaat cacaattaat ttcttatgaa aagtatcaat ttaattgggc aaaaaaatat
    17281 ctattaaaag gagaagttaa tgaaaataaa ctacagtcag aaaaccttgc attagtagaa
    17341 gaggggcatg gatttgatat aggagataag atatatatta aaaatagaaa tgctgaaaaa
    17401 gagttagagg taggaggtat actttcctca attccatttg aggctaaaga taaatatatt
    17461 ggaaatatta taacatctga aaatactttt acgaatataa caggtataaa gggttatata
    17521 ataatggata ttcaggttga taaagatact cctggagatt taattgaaaa tataagacgt
    17581 gtagtagatg ataacttaga ggtcagagat aaaagacaaa gtaacttaga ggttaaaaat
    17641 tcgttttata ctatggcaac atttgtatat ggatttgtgt ttattattgc gattataagc
    17701 atgtttaata tcataaatag catgaatata agtgttacaa gtagaaagaa ttactatggt
    17761 gttatgcgtg cagtaggcat atccaacaga caattaagaa gaatggtaat agtagaggca
    17821 ggttcatatg caatgagtgg ttgtttatta ggtagtatac taggattaat gttacatagg
    17881 tatatgttta tatcgttagt aagttcaatg tttcatttag agtggcaagt accttttaat
    17941 ttactactta tcactgtact aactatgata ataataactt tattatcagt aagaaaacca
    18001 gtcaaaaaga tttgtgaatt agatataata gactcaataa atgcacaata aatatagctg
    18061 ttgatttaaa ttaaaaaaac gaagtaaaaa tgaaatagaa aatatttttc atttttactt
    18121 cgttttttat attataaaat ttttgttatg ttacaaaatt tctataaaga tgatgtgaat
    18181 ctaaacatta tatgacaacg tgagcattta agttaaaata ttttaaatat actattttag
    18241 ataagttcct tcttcatctt gataaattaa tccttttttc ataagcactc caagagctcg
    18301 tttaaaattt ttcttacttg aattaaataa tatagaaata tctttagggt cagatttatc
    18361 attaaatctc atatatccat cagaaccctc taaataactt aaaatcttat tttctaaagt
    18421 atcaagttca tcctttctat ttcctctagg agaaagacca agttttccat cttcatatat
    18481 ttttatgaca tgtaaatttt caagaacatc accagctttt agttcagtaa agtactcatt
    18541 atgaagtatt acaccagcat atttattgtc tacacatatc attgcagaat tattagtttg
    18601 aaatccatat acaactccag ttacaatatc tcctacattg taagtatggt ctgttaataa
    18661 atatgaatct atatctgtag tagcagcaat tcttttgctt ttgtctaaat atatataaaa
    18721 taaatatttt tcgtctctaa atagtggata agtcttagct ttaaaaggaa ctagtatgtc
    18781 tttaggaagt cccatatcta taaaagtacc aatgtccgta ttatcaacta cttttaaatg
    18841 tgctacatcc ccaaccttag ctaaaggcgg tattaaagtt gcagcagttc tgtcatcaga
    18901 atctttaaat atgaaagcat ttacttcatc acctatgttt atttcatttt ttccgattaa
    18961 tctattatga agaagtatat cgtcttttgt gttattagtt tgtccatcca aaaaatatcc
    19021 aaattcagtt tttcttttta ctgtaagttt attaaaatca cctatgttta tcaaattaaa
    19081 atcatcctct cattttatat ttataattta gccataatat acttatgtag tagttt
//
LOCUS       DACTGW010000058        19076 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.058, whole genome shotgun sequence.
ACCESSION   DACTGW010000058 DACTGW010000000
VERSION     DACTGW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.058"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            181..759
                     /locus_tag="I9573_001588"
     CDS             181..759
                     /locus_tag="I9573_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HAT4796214.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            798..1376
                     /locus_tag="I9573_001589"
     CDS             798..1376
                     /locus_tag="I9573_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HAT4796215.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLRHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1462..2091
                     /locus_tag="I9573_001590"
     CDS             1462..2091
                     /locus_tag="I9573_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HAT4796216.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2081..4840
                     /locus_tag="I9573_001591"
     CDS             2081..4840
                     /locus_tag="I9573_001591"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HAT4796217.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            4927..6489
                     /locus_tag="I9573_001592"
     CDS             4927..6489
                     /locus_tag="I9573_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796218.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDIHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6490..7626
                     /locus_tag="I9573_001593"
     CDS             6490..7626
                     /locus_tag="I9573_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAT4796219.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7690..8766
                     /locus_tag="I9573_001594"
     CDS             7690..8766
                     /locus_tag="I9573_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HAT4796220.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALYSMNLQDMYVI
                     PIIETREFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8744..9982
                     /locus_tag="I9573_001595"
     CDS             8744..9982
                     /locus_tag="I9573_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796221.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGVRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELNCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKKHYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10036..11124
                     /locus_tag="I9573_001596"
     CDS             10036..11124
                     /locus_tag="I9573_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HAT4796222.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11152..11943
                     /locus_tag="I9573_001597"
     CDS             11152..11943
                     /locus_tag="I9573_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAT4796223.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKPDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDFSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFKGMSGTEEILSNILDENCLLNI"
     gene            complement(12058..12612)
                     /locus_tag="I9573_001598"
     CDS             complement(12058..12612)
                     /locus_tag="I9573_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAT4796224.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12807..14009
                     /locus_tag="I9573_001599"
     CDS             12807..14009
                     /locus_tag="I9573_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HAT4796225.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14066..15046
                     /locus_tag="I9573_001600"
     CDS             14066..15046
                     /locus_tag="I9573_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAT4796226.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15293..16246
                     /locus_tag="I9573_001601"
     CDS             15293..16246
                     /locus_tag="I9573_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAT4796227.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAVFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16236..17192
                     /locus_tag="I9573_001602"
     CDS             16236..17192
                     /locus_tag="I9573_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAT4796228.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17189..17944
                     /locus_tag="I9573_001603"
     CDS             17189..17944
                     /locus_tag="I9573_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT4796229.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18036..19001
                     /locus_tag="I9573_001604"
     CDS             18036..19001
                     /locus_tag="I9573_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT4796230.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKTSLPASLEKYKDKKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         7543 a         1934 c         3417 g         6182 t
ORIGIN      
        1 aataaagtat gtaaaatcta tgtaaaaagt aagaaatata aacatttttt taattatatt
       61 ttaaaaatat caagcaagtt ttcttcacaa atgaataaaa aatgttataa tatgattaaa
      121 gaaaaagata aataattagc aagatttgtt tagctaaaat taacaaggga gcgataaatt
      181 atgggtataa cattatcaaa aggccaaaag gttgatttaa ctaaaggaaa tccaggtctt
      241 aaaaatattt tagtaggttt aggatgggat actaacaagt atgatggtgg atttgacttt
      301 gatttagata cagcagcatt tttaacagga gcaagtggaa gtgttactaa tgatggagat
      361 tttgtatttt acaacaattt aaaacataca tctggagcag ttgagcatct aggagataac
      421 agaactggcg aaggtgatgg agatgatgaa caaatagttg ttgatttatc taagattcct
      481 ggagaaataa gcaagatttc ttttactgtt acaattcatg atgcgacaga aagaagacaa
      541 aattttggac aagtaagtaa ttcttatata agaatagtag ataaagacac aaatgaagag
      601 ttaataaagt atgaattagg agaagatttt agtatagaaa cagctatagt agtagctgaa
      661 atatataagc acaatggaga atggaagttt aatgcactag gttctggttt tgaaggtggt
      721 cttgctgcat tatgtggaaa ctttggaata aacttataat ttcaactata acaataaact
      781 aaagggggat attttccatg gctatagtat taaaaaaagg acaaaaaatc gatttaacta
      841 agggaaatcc aggtcttaaa aatataaaac ttggactagg atgggataca aattcatttg
      901 atagtggcta tgattatgat ttagatgtaa gtgttttcat ggttggagaa tctcaaagag
      961 ttgaaaaaga tgaagatttt atattctaca ataacttaag acatccatct ggagcagttg
     1021 aacacttagg tgataataga actggtgaag gtgatggaga tgatgaggaa atattagtga
     1081 atttagagac aatgccaaag catatacaaa agatagctgt aacagttaca atatatgagg
     1141 ctgctgaaag aagacaaaat tttggacaag taagcaactc atatataaga gtattaaatt
     1201 ctgaaaatga tgaagaaata ttaagatatg acttaggtga agaatttagt atagaaactg
     1261 caattacagt atgtgagata tataagtata atggagaatg gaagtttagt gcagttggag
     1321 caggatttga aggtggctta gaagcacttt gtagaaacta tggcttaaat gtttaattta
     1381 agcaatataa tatattaaat ataaataacc tatacaaata atgtataggt tatattatat
     1441 aaaattttgt ggaggtttag tatgtcgatt aatttatcaa aaggtgataa aatagattta
     1501 aaaaaatcta acccaggact gagtaacata cttgtaggtc taggatggga ccctgtgcaa
     1561 caatctggag gaggtttttt taaatcgtta tttggtggag gacaagctga tatagattgt
     1621 gatgcatctg tatttatgct taatcaagaa ggtaaattaa gtggaataaa agatttaatt
     1681 tattttggaa atctaaaaag tgcctgtaaa tcagttttgc atacaggaga caatctaact
     1741 ggtgagggta caggtgatga tgaacaaatc ctagttaatt tggataaagt tccatcaaat
     1801 attcataaat tattgtttgt agtaaatata tacaattgtg tagatagaaa acaacacttt
     1861 ggaatgatag aaaatgccta tataagagtt gaagaccaag gtaataagaa agagatagca
     1921 aaatacaatc tatctgataa ttattctgaa aaaactactc ttatagtagg agctatatat
     1981 agaaaagatg gaagttggca atttaaagca attggtgaag gaacaaaaga tgcgggtctt
     2041 aaagaagtaa tgcaaaattt agacaggatt gagtgtgcat atggaattta ataatgatat
     2101 caaatattat aatgtagata aagaagaagt tacaaaagac ttatctgtta atcctgaaaa
     2161 aggtttatca gaatcagaag tcaagactag aagagaaaaa tatggtttga atgaatttac
     2221 tccaaaagaa gagggtagtt tttgggatga tttaaaagaa agtttaagtg aaccaatgat
     2281 agttatcttg attattgcag cagtagtaag tgcagtaatt ggggaaactc atgatgcaat
     2341 agggattgta ggtgctatag caataggtat agcaataggt atgattacag aaggtcgttc
     2401 taagaaagca gcagaggctc tttctaagtt gactgaaaac atagaagtaa aagtattgag
     2461 agatggggaa gtacatcaaa tttctaagag tgaattggta ccaggtgata tagtatatat
     2521 tgaaactggt gacatggtac ctgctgatgg tagacttata gaaagtataa accttaaaat
     2581 tagagaagat atgttaacag gagaatctga tgacgtatct aaaaagtgtg atgtagtagt
     2641 ttctatggaa gagatagaat ctaaaggagc agtagtagta caggagccaa tacctgcaaa
     2701 acagataaat atggtgtttg gtggtacact tgtagcttat ggtagaggag ctctagttgt
     2761 aacttcaatt ggagataata gtgaaatggg taaaattgct caaaacttat cagaaacaga
     2821 tgagcaaact ccacttcaga taaaacttgg aaatttaggt ggtttgatag ccaaagtttc
     2881 tagtgcagta gcaggattac tatttatatt tatggttttc caaatgataa gtaagaatgt
     2941 attaaatgta gatatgtctg gaatattacc gtttttagaa tcaatagacc cagttaaaac
     3001 agcatttaca gtatgtgttg cgttgatagt tgcagcagtg ccagaaggtc ttccaactat
     3061 gataaatatg acacttgcaa taacaatgca aaaaatggct aaaataaatg cattggttac
     3121 caaaaaagaa gcttgtgaaa cgataggttc tgtttctgta atatgttcag ataaaactgg
     3181 tacattaact gaaaatagaa tgactgtaga agttgcatat gttgatggca gatatattga
     3241 aagttcagaa gaggaaataa atagttattt tgaagaaaac tgtatgataa actctacagc
     3301 tgatgtagag cacaatgatg gtgatattaa gtatttagga agtgctactg aatgtgcatt
     3361 gcttttatat tacaaaaacg tagattatag acaagttaga aaaaattcta atatagtagc
     3421 tcaaaatcct tttacttcag attcaaagag aatgacttct gttataggtc aagataatca
     3481 tcatgtacta ttatcaaaag gagcaccaga agtcttactt gaattatgtt cacatattca
     3541 aagaggaaaa gacatagttc cattaactga aaatataaaa catgagattt tagaagaaat
     3601 aaagaaattg caaataaaat caatgagaac tttagggttt gcatataaag aaatctcaca
     3661 agctgaagaa gaagcagcag taactgctga aagtgatgct atgaatgtaa gtgcaatgga
     3721 aaataattta gtatttagtg gttttgttgg tataagagac ccacttagaa aagatgttat
     3781 agagtctgta aatacagcaa ataaagctgg tgtttctgta aaaatgctta caggagataa
     3841 tataaatacg gcaagagcta taggtgaaga gttaggccta ttaaaaaata atatgagagt
     3901 ggttgaagct tcatatatag atactttaga tgatgaagaa ttaaaacaag agatacaaag
     3961 tatttcaatc gtggcaagaa gtaaaccaga tagtaaaatg agaatagttt cagctctgca
     4021 aaagagcggg gatgttgttg cagtaacagg tgatggtata aatgatgctc cagcactttc
     4081 aaaagcagat gttggtatag caatgggtat atctggtaca gaggtatcta aaaacgcagc
     4141 agacataatt ttaactgatg atagttttag taccattgta aaaggtatta aatggggtag
     4201 aggaatttat gataacttcc aaagattcgt tcaattccaa ttaactgtaa atgtaattgc
     4261 atttttagtg gctataatat ctcaagttat gggacaagag atgccattta ctacaataca
     4321 attattatgg gtaaacataa ttatggatgg accaccagca ctagcattag gtttagagcc
     4381 agttagagac catgtgttaa atagaaaacc tgttgataga aaagccaata taatttctaa
     4441 gtcaatggtg tatacaatag tattaaatgc attttatata acagcaatac taatgttaca
     4501 atctacattt aatttcttag gagttgatga agctcataag ggaacagtaa tgtttgcgtt
     4561 atttgctttt agcgcactgt ttaatgcatt taactgtaga gagtttaatg cagatagtat
     4621 cttccctaat tttacacata acaaattagc actacaaata atagtgttga caggaattgc
     4681 acaagtaata tttactcagc tattccaaga tttctttaac tctgtagcaa tggattttat
     4741 gacatgggta aaagttttat gtgtagcagc aagtgtaatt gtggttaatg aagttgtaaa
     4801 atgtataata agactggtaa aaccaaaaaa gactgcttaa aacaaatttt ttagaagaaa
     4861 acagactgac tcaaaatatg aattttgggt cagttctgaa taaacattta aattgggagg
     4921 atagttatgg gtgactcaaa ggtatttgaa aaaatattct ccagccaatc agatagagga
     4981 aattacactc cttcaaaggg atatctaagt tattttattt cgtatattgg tttagaagat
     5041 gaagttttgt acaatctcga gatatttaag acaaaacaaa atattgatag caaaaaagat
     5101 attgctttat ttactgatgt tatagcaaat cctagtgatt ttgatattat aaattatttt
     5161 aaaagtggac ttcaaaaata tagaactagc atggaagatg tagatataaa tattttggga
     5221 tttgaagaga ttgattataa aataaagcaa gcaatggata gagtgttaaa agaagaggaa
     5281 aaagaattta ctaatgatag agttaaacaa aattttatag tgaaaataat ggcatggata
     5341 aaaatttata tcggtgcttt agatataaat aaaaatgaag ctccaaaagt aatattttat
     5401 ggagatataa aaaaacatga agtttattta ctcttaatat tatacttggc aggttttgat
     5461 gttttatatc ttaatccaaa ttcaaagagt aatatagata ttttaaaatc agaaagatac
     5521 aatatagagt ttgaagaagc caacataata gaagaaaaaa tttcttttga agaaagggtt
     5581 attttagggg aaaaaataga taaaagttct gtaaaaaaag cttttactgt tggggcagaa
     5641 gcatcaaagc gtataagtga ggaattgtta aatgatgcag gatttataaa accttggcaa
     5701 ctccaagata gaaaaataaa aaatctactt ctaagttcaa ctgttgatga aatatcaata
     5761 tactggaatc aaccacttaa attaagacca ggatttaagt ttaatgatgc tattgtagaa
     5821 gctccaaatt ttctttctaa gattaatgga atatataatg ataagaatga atatattaag
     5881 tttttagatt tattaagaga ttcggaaagt tcaacattta ttgaatttaa tggagatgta
     5941 gatagatttt caaaagcttt tacgagagaa gcttttagtt tatccttttt attggattca
     6001 aaaggtgtaa tagataaaaa ctctgtttta aataataaag attattccat atcaacacta
     6061 gcacttaatc aacaaattat gattttagag aaagtggaag agttgttaga aggaagtatg
     6121 tttttgaatg gtttaagtgg agaagacaag ataaaaggtc tatttacagt acttcacatg
     6181 gataagaaat ttgttcatat gatgaacaat ttcgattact cacttataaa cccaaaattg
     6241 attatataca tgtacaagag tattgtattt gataaagaaa tagtttttct tatgctttta
     6301 cttagtaaga ttggatttga tattatcata ttatgtccag gaggagaaaa caatatagag
     6361 aatgttataa ataaccaatt gattgatata cacagattgg ataaaatggt atatgacctt
     6421 aaattaaatt cgcttgaaaa tgatatacct ttacttaaaa aaatttttgg aaagaggaga
     6481 cgattttaga tggggataaa tataaatagt ttttcaaaag ataagaactc tgtagaggtt
     6541 actgagaatt tgcctacagc agttcaagaa gaaaacttcg atataatgga atatacaaat
     6601 aataaaaaaa atgaactgag aaagtctaag gaagttgagg cattgacatc ccttatagaa
     6661 gtagaaaatc cagatacgat tttacagttt ggtaggaaag catctgaagg agtagcaaga
     6721 gtttctgata gccttttaaa tacaataaaa ttaaatagaa atgaagaaaa ttctaaaatg
     6781 ctggttcatt taactaaaat aatggataag tttgatttag acgattttca agaaactaag
     6841 gaaccaaatt tcgttcaaaa acttttcaaa aaagctaata atgccattga aatgatgttt
     6901 caaaagtatg aaacattggg aggagaagtt gagaaaattc aaattgagct tgagcaatac
     6961 gaaagagata tagcactttc taataagcaa ataggtgcta tgttaaatga aaactttgag
     7021 ttttataatg aacttcaaaa atatattgtt gctggagaaa tggcaataga agaaatggat
     7081 aatgaaatac tacctttctt taaacaaaaa tcagagacta gtggagacca aatggatgtt
     7141 gtcaattatc aagaactatt aaaagtttat gatatgttaa atcaaagagt ctatgattta
     7201 agaatagcag aaaatatagc tatccaaaca atacctatgc ttagaggtat gcaacataat
     7261 aattatggtt taattagaaa aataaatagt gcatttgtcg taactttacc agtatttaaa
     7321 caatgtttat ctcaagctat attacttaaa aagcaagaat tacaagcaaa atctctaaaa
     7381 gcacttgatg acaaaactaa tgaattattg cttagaaatg cacaaaatgt aagtactcaa
     7441 agtgcacaaa tagctagaat ggctggaaca agttctgttc aaatagaaac attggaaaaa
     7501 atgtacaata ctatcaagtc tggtatagat gaaactatga gaatagaaga aaacaataga
     7561 gctttaatta aggataatac aaagagattg gaagaattaa atacgactat aatttataat
     7621 aagtagtatt atattaaggg ctaatttgta taaagttggc cctttctatg tatatgagga
     7681 ggctagacat tgaaatattt cgattatatc tgcaaagatg atttggagag aatttttttg
     7741 aaggagccag aagactttag tgctaagact gaaaaagatg tgttgaaata tgcactgggc
     7801 gcttttttat acgttccagc tactcaatat aatatgattt ataagagtat tataggggat
     7861 gttaagggag ttagaccatt agctatttgt ttagaagatg ctgttggagt aaatggagag
     7921 ttagaggcta tagaaaatct tagattaatt ttaaaaaata ttagcaatga aagtataact
     7981 aataaagatg gaataccact aatttttgta agaataaaag atgtggagca actgttgaga
     8041 attaaagaga ttataattaa aaatagtcat tctataacag gaatattaat tcctaaagca
     8101 aattcggagt tgattgaaaa ttgtatagaa gctttatatt ctatgaatct tcaagatatg
     8161 tatgttattc caataataga gacaagagaa tttatatata atgaaaaaaa agaacttagt
     8221 tttactaatt tgtacaatgc tattttaaga cataagtcaa gaatattaag cattagagta
     8281 ggcataactg atatattagg gatgtatggt ataaggagag ataaaaactt ctctatatat
     8341 aataatttga tttgttcttc atttatatta gatataatca catatcttca aagaccagag
     8401 ttagatatac ctataagtgg tggagtatca gaattttttg atatgacaaa taaggatata
     8461 agaaataagt atatagaaga aatattattg gataaatatc atggattaat tggaaagacc
     8521 gtaattcatc caatgcaaat tcaaattgta caagcgctat ctacagtatc atatgaagat
     8581 tttactgatg ctttggatat actggatagt acagacagta agtatggtgt gagcaagggt
     8641 gtattaggag agagaatgaa tgaaacaaat ccacattttt tatgggctaa aaaaacattg
     8701 attctatcta aaatatatgg tgtattaaat aaaggagtag actatgaaga attgcttaaa
     8761 ttttaattgt gagattgaca ttattgataa tccattgaat ttagaactaa atgatttttt
     8821 agacgtagga gtaagaaata atagcaaaag acgttttttg tttatctcaa agaaactcgg
     8881 taaacattta gcatgtaagc caagtgagat ggataatttg ggaaaattaa tggctacaat
     8941 ttataatgaa aaaaataagg aagaatcaaa tggtactgtt atagcttttg ctgaaactgg
     9001 tacagctgtt gggcatagtt tttttgatta cttatgtgga gattatgaat ttattcatac
     9061 tactagagag cagtttgacg agttagaaaa tctggaattt ttagaggaac attcccatgc
     9121 aacagaccag aatttatact ttggtatgct taacaacttt aaatttggag atgagataat
     9181 attggttgat gatgagatta ccacagccaa tacatgcatg aatataatta ggaaaattca
     9241 aagcatttat cctaaaaaga aatacactat atgctcaata ctcaattggg taagtgatga
     9301 aaatttaagt aaagttactc gtttagaaag agagctaaat tgtaagatag attttgtata
     9361 cttatttaaa ggaaacttta aattttatat agatgaagat attatttcaa attacaacga
     9421 taattatagt attactaaat tagagactat ttcaagtgat aaagtaaatt tagtagtaaa
     9481 tcatatttat gttgatttag aagattatat tggaaataag aaatatgtaa aatatacagg
     9541 aagatttgga attaatagaa gagaacaatt tagattaaaa gaaatcgtta agagagaaag
     9601 taaaaaaata actatggcca gtaaagaatc tttaaaaaat gaaaaagaaa agattctatt
     9661 tttaggtact gaagaattta tgtatatacc tatgctgttt gcaaaagaaa aagaaaatca
     9721 atatgatata tactatcatt caacaacaag aagtccaatt attaatattg ataagaagca
     9781 ttatcctatt aaaagtaaat ttatgctaaa gagtttttat aatatagata ttcaaaatta
     9841 tatttacaat atagataaac ataatttttc taagtgttat ttatttgtag agctgaataa
     9901 aagtgaaaat tcttatctag aatttattga tataattaaa aaaacaagta taaaagaatt
     9961 aactatagta tgttgtagct aaagtgtttt aacaggataa aatatatcac gattttaaga
    10021 taggagttga gtcatatgag agattttagc acgtattcta aagatgatgt tattttttta
    10081 ttaaaagata tatcaaattt aattgaagaa gaaggaaata aagaaagaga attaaaaata
    10141 caaaatggta gacattattc agaaatgata ccaatagagt atgaagttag tgaagattat
    10201 ctgaatttat atcatgagaa actaagagaa aataaagata agcttgcatt ttctatagga
    10261 gttatgtgtg aaaaaataat taaaaaaaat ggaaatgatg tagtactagt ttcacttgct
    10321 agagcaggaa cacctattgg tattttggca aaaagatata taagaagcaa gtataatgta
    10381 agtttacctc actatacaat ctctataata agagataagg gaatagatat gaatgcaatt
    10441 gaatatatag taaagaatca tccgagctca aaaattcaat ttttagatgg atggactggt
    10501 aagggaacta tatcaaaaga acttgaaaaa gcatgtgatg aacttaatgt taaatttaaa
    10561 aaacattttg actcaacact tgctgtatta gctgaccctg ctggatattc aggactttat
    10621 ggaattagag aagatttctt aataccaagt gcatgtttaa attcaaccgt ttctggttta
    10681 gtaagtagaa ctgtactaag agaggattta attggtaaaa atgactttca tggagctaag
    10741 ttttatagac atttaaaaga taaagatgaa tctatgaact atatcgaaac tatagaagaa
    10801 tgttttaaaa atcaatttaa gaatatatct gatgaagttg aaaactggga aagtgacatt
    10861 ataacaagag atggatactt tgatgtttta aatataaaag aaaagtataa cataacagat
    10921 attaacttta tcaaaccagg tgtaggggaa acgacaaggg ttttacttag aagagttccg
    10981 tataagattc ttgttaaaga cttaaatgat aaatctttag accatatatt catattagct
    11041 aaggagaaaa atgttgaagt tgaacaaatg gatttaaaag cctataaatg ttgtggaata
    11101 ataaaaaata tgaaagatat ttaggaaata tattagacag gagaatattg aatgattgtt
    11161 ttttcagatt tagacaggag tatcatttac tccaataaat ttttaaatgc tgatagtaaa
    11221 tatgctaata tagaaattta tagggaaaaa gaaatatctt atatatcttt agatactata
    11281 aatttaataa agcaaataca atattatggt atgtttatac ctacaactac tagaacagtg
    11341 gagcaattta agcgtataga atttaataaa tatgggattt attttccttg gagtataact
    11401 tcaaatggag gagttatact taaagataat gaaatattga aatcatggag tgaaaaaata
    11461 gataaactaa aacctgatta tgagcccatt gaaagtatga ttcataaatt taaagactat
    11521 ttgaatgtag atggaataac taattttaaa gttgcagaag atacattttt ttatatagtt
    11581 gttgatttct caagatttaa tttggattca atcaaagaat atacaaatat attggaatca
    11641 aaaaattgga agttttatgt gagtggcaga aaaatatatt ttataccaaa agaaatttca
    11701 aaagaaaatg ctataaaata cttgactaaa gaattaggaa tagaatattt ttatgcagtt
    11761 ggagattcta ttatggacta tggtatgctt aatatctcaa ataaatccta tgtattgaag
    11821 catggtgata taaataaaaa tgaaatagaa aattcattta tatcaagttc tttcaaagga
    11881 atgagtggaa ctgaagagat attatctaac attttagacg agaactgcct acttaatata
    11941 taatgactga tatataaata ataaactaga aaaatctgaa ataaattata gtaaagttta
    12001 tcatactaat ttatttcaga tttttatcaa tttatattta agtaagaact caatatgtta
    12061 aaatgaaggt ggagtcattg cccagactga gatacatacc tcatcatcat aattttcata
    12121 cacatgtggt atagtactgt taaaataaaa actatctcct tcctccaata gatattcctt
    12181 atttccaagt gttatcttca ttttaccttt tattatatag ccacattctt ctccttcgtg
    12241 tgtcacaaat tcactgtttt tttgtgtatg accatctagc gtaacaagta taaattctat
    12301 ttttttccca gataaattag gaacaagaag ttcataaaat tttgtagaat cattagttac
    12361 tatttttttt cgattatttt tctttactac tatatcagag tcctctcttc caatctcttc
    12421 aaaaaaataa cctatattta tatcaagagc ttttgatatt ttccacattg ctacaataga
    12481 tggaacaacc atattacgct caatttgact tattaagcct gtacttaaat ctgaattttt
    12541 tgctaaagta gagatactta tatttttaga ttttctaaga gatctaattt tgcttccgac
    12601 atctatattc attagataca aaccccctca gacatgatta taacatataa tttataaaga
    12661 ttaataaaat aaaaaaaagt tttataatgc atgtattttt tatgttatat tgaattttct
    12721 gaaaagatgc attataatag tattaatatt caatatattg aatattgata atgatatttt
    12781 aatttaatga aatggaggta attaagatga agagttttgt aagtcctgat aatatttggg
    12841 tattatgggc agttgtaact ggatgggcag catttagtat ttacttggaa caaaagtaca
    12901 attgggcatc aaaagtgtcg ggagcaataa tagctcttgt aggagcaatg ttattatcaa
    12961 atctaaatat tataccagta gagtctgttg tatatgacca agtatgggga tatgttgttc
    13021 ctttagccat agctttattg ttatatcaat gtaatattaa aaagatatgg aaagaaagtg
    13081 gaagattgct cattatattt cttgtaggtt ctgtaggaac tgtacttgga gctatgattg
    13141 gatttttagc attaaaaaat gtagtaccag atttaaatat tgttgcagct atgatgacag
    13201 gttcttatat aggtggaaat gtaaactttg ctgctatgtc aggggcgttt gatgcgccag
    13261 gagaattagt atcagcaaca gttgtagcag ataatctttt aatggcatta tatttctttg
    13321 tcttaatagc tataccatca attggatttt ttagaaaaca ttttaaacat ccacatgtag
    13381 atgaaatgga aagtataggt ataactgaag gagaaacagt agcagcaaat tattggggaa
    13441 gaaaagaaat atctttaaaa gatatagcaa tagccattgg tagcgccttt ataattgtcg
    13501 cagtatctgt agaactatca acttggttta aagctataat accattatca aatccttttt
    13561 tagcaatgtt aaatacttta tttggaaatc aatatctaat aataactact ttgactatgt
    13621 tgtttgccac atttatgcct aatttctttg gtgatataaa aggagcacag gaattgggta
    13681 catttttaat atatatattc tttgttgtag taggtgttcc tgcttcaata acattaataa
    13741 ttcaaaaatc accattacta cttttatact gcggaataat ggtactaata aacatgctag
    13801 ttacatttat agttgcaaaa atatttaagt ttagtttaga agaagctatt cttgcatcta
    13861 atgcaaatat tggtggtcct acaacagcag cagctatggc aatatcaaaa ggatggtcta
    13921 agctagtagg gccaatactt atagttggaa catttggata catagttggt aattattttg
    13981 gtcttttagt aggaaatatt ttgatataat tttacgtgaa aagaagttgt tttttagaaa
    14041 gatttttatt taattggagg ttgttatgat tttcgatgga cattcagata cttgggcaga
    14101 tgtaacaata agaaggctta acggggaaaa agatgtattt agaagaatcc atcttgataa
    14161 ttttaaaaga gcaaatatag aaggagctat ttttgtaata tggatagacc caccatatga
    14221 taaagagcct gaaaaaagag ttaaacaaat tataagctct ataaaagatg aaattgaaga
    14281 tatgaaagat gttgtaaaca tagtaaaaaa atattcagat atagataaag gtataagtga
    14341 aaacaaaata aatattttaa ttggtcttga aggattaagc catattggaa gaaatataga
    14401 tttactagat tatttttata aagaagttgg tgcaagacat gcatcattaa cttggaatga
    14461 acagaatgaa cttgctacag gatgtttagg ggatgaaaat cgtggtttaa ctgaaatagg
    14521 taaaaaagca gtcaataaaa tggaaaaatt gggcatgata gtagatgtgt cacatttaaa
    14581 tgaaaaatca ttttgggatt taatgtctgt tgcaagtaag ccagttatag cttctcattc
    14641 taatgctagg agtttatgtg atgctaaaag aaatcttaca gataatcaat taaaagaaat
    14701 ttcaaataca ggtggattag ttggaataaa tagttttaga ggatttacta atagtaatcc
    14761 taagaagcaa gatttaatac atttggttga ccatatagac tatatggtaa atcttatagg
    14821 tatagaacat gtatcttttg gttttgattt tagtgattac ttagggaaag agactatagg
    14881 ttcttttact ggaaatgcta gtgtgccaag tgtagatggt cttgaaagca cacatacagc
    14941 acagaacata gtagtagagc ttgaaaatag aggttataaa aagaaagata tagagttaat
    15001 ctctagtggt aatttttata ggctctttaa agatatacta aaataatata ataaagaact
    15061 taaatttata aaatagtata tttatacaat aatatctaca tatttaaatt ggcgttcata
    15121 atttattaaa ttaaatttta atgttactag aagtttcaat tctagtaaca ttttttatgt
    15181 taaaaataga tatagatata tattgacaat gataattatt attgataaac tataaaagtg
    15241 aaaatgataa taattttcat tttaattaat aaaagatttt taaggaggga gaatgaagaa
    15301 aaggtattta gtcataggaa taataatttt gtcttatttt tctttattta taggagctga
    15361 agatataaat ataatgcata tatttgcaaa aaatcagcat aagcttatga tatttatcat
    15421 gagtagggta ccaagattaa taagtatatt gattgctggt gtaggaatga gcatagcagg
    15481 tcttataatg caacagataa gtaagaataa atttgtatca cctacaacag gtgctacaat
    15541 agatgcagct caatttggga tagtaatatg tatgctttta gtaccaactg cctctatttt
    15601 tacaaagaca ataatagcat ttgtattttc tcttgtaggg acttttacgt ttatgaaaat
    15661 aatagggaag ttacaattta aaaatataat ctttgtacca ttagtaggta taatgtttgg
    15721 aaatataata ggttctatga cggattttat cgcgtataaa tacgatttga gtcaaaatgt
    15781 tagttcttgg atgcaaggtg acttttctat gatacttaaa ggaaattatg agatattata
    15841 tatcacaata ccccttataa tattagcata tatttatgca aataaattta ctgttgttgg
    15901 tatgggtatg gattttgcga caaacttagg tcttagttac aaaagaattg tcaatatagg
    15961 tctgataata gtagcacttg ttacagtatg tgtagtcgtt acagctggta atatcccttt
    16021 tataggtcta atagttccca atatcgtatc cttgtatatg ggtgataata taagagcaag
    16081 catatggtat actggtttac ttggagcggt atttgtactt atatgtgata tttttggaag
    16141 aattattatt tatccatatg aaatatctat aggacttaca gttggagtta taggaagtat
    16201 actattcctt tatttaatat taaggaggaa tgtgaatgaa gcttagtctc aatcaaaaaa
    16261 tttactttgt aagtatatta ctattcatat caatagcact ttttataatt tatggaatag
    16321 acatgaatca tttagaatat gcattatcac aaagaatacc aaaaatattt gctatggtat
    16381 taggtggagg atgtatagca tttactactg tagtgtttca aacaattaca aacaatcaaa
    16441 tattaacacc aagtgtacta ggtttagatt cattgtatgt aatgattcaa actataatag
    16501 tattcatatt tggttcaagc agtagtctga ttataaatga aaattataat tttattatta
    16561 atgtagctat gatgattgga gcttcattgt tattatataa gatgttattt gaaaaaaata
    16621 aaaataatat tttcttttta atcctagtag gtatgatatt tggaacctta tttaaaagtg
    16681 caacaacatt tattcaagta atgatagacc cgaatgaatt tttagcttta caaactagta
    16741 taatggcaag tttaaacaat ataaacacta atgtattgtt aatagcattt ataataatta
    16801 ttgccataat accttttata tatgatgaaa taaagtattt agatgtattg tcgttaggta
    16861 aagaacaagc aattaattta ggtgtagatt ttgataaggc agttaaaaaa atgataatat
    16921 taatagcaat tttagtctca atatcaacag cacttattgg acctatgaca tttttaggac
    16981 ttttacttgc taatataaca agagaaattt ttaaaacata taagcataca tacttgattt
    17041 cgggttctat gttgataggg atgattacat tgataatagg tcaatttttt atacaacatg
    17101 tatttaagtt tgatacgact ttaagtgtag taataaattt tataggagga atttacttta
    17161 ttcaattact attaaagggg gcaaatagat gatagagata aaaaatatat ttaaaagata
    17221 taagaataaa aatgttgtag atgatgtctc ttttagtata gaaaagggaa agataacatc
    17281 ttttatagga ccaaatggag ctggaaaaag tacagtacta tcaatagtta caaggcttat
    17341 aggaggtgat ggcggagaag taataataga aggaaaaagc ttgactaatt atagcaataa
    17401 agaattagca aaaaaaatag ctattcttaa acaatctaac aatataactt taaagcttac
    17461 aataagagaa ttagttggat ttggaagatt tccatatagt gaaggtaatc taactaaaga
    17521 ggatgaaaat tatattgatg aagcaataga atatatgaag ttgactgata tacaacataa
    17581 gtatttagat gaattgagtg gaggtcaaag acaaagagct tatatagcaa tggtaatagc
    17641 tcaagataca gagtacatat tacttgatga accactgaac aatttggata tgaaccattc
    17701 cgtacagatg atgaaggtac ttagaagtct ttgtgatgag ttagataaga ctatagtatt
    17761 agtaatgcat gatataaatt ttgcatcatg ttattcagat aatatagttg cattaaaaaa
    17821 tggaaaagtt gaaaaagtag gtcgtacaga tgagatagta aatgaaaaag ttttagaaga
    17881 tatttatgag atgaatttta acattaagaa tataaatgga aatcgaatct gtatttattt
    17941 ttaaaatatt gtatatgaaa ataagcttgc tatatgattt tttataaagt ttttaaatta
    18001 aaataaagat taataggaga gaggaaaata ttgatatgaa taaaaaagca gcaatagtag
    18061 cagcagtagc tattatagga ttagtgacag tgtttgcatt aggtggaagt aaaaagaatg
    18121 aatcaaaaac atcagaaaat tcaaataata ccataaagat aactcataac ttgggagaaa
    18181 cagatgtaaa attaaatcct aaaaaagtag tagtatttga ttattcggct ttagatacaa
    18241 tggatgcatt aggtgtagct gagaatttag taggacttcc aaaaacgagt ttaccagcat
    18301 ctttagaaaa atataaggat aagaagtatg cagatttagg aggtttaaaa gaaccagatt
    18361 tagaaggtat aaagtcggca aatccagatt taattataat aaatggaaga caagaagatt
    18421 tttatgaaca attgtcaaaa atagcaccaa caataagtac aagtaaagat gataagaagt
    18481 acttagaatc tgttaaaaat aatatagaca aaatagcaaa aatatttgga gtagaagaaa
    18541 aagcaaatca agaatttagt aaaattgaaa agaaaataga aactttgaat aaaaaagtaa
    18601 cagataaaaa tttaaatgct ttaactataa tggtaaatga aggtaattta agtgtatttg
    18661 gtgaagaatc aagatttagt atattataca atagttttgg atttgaaaat aaagataaaa
    18721 atataaaaga atcaagtcat ggacaaaata ttacttttga atatatagct aaacaaaatc
    18781 cagaagttat gttcgttata gatagaggaa tagctactgg tagtgatgta aaagaaagct
    18841 caactgcaaa atctgttttg aacaatgata taataaaatc tatggatgca tataagaatg
    18901 ataatataat atatttagat tcaccaacat ggtatgtaaa tgatggtggt ctaacatctt
    18961 taaataaaat gatagatgat gcttcaaaag cggtaaatta ataatccgat ataatcaaaa
    19021 tgtatttttt gtaaaatgat tatcaaataa actaggagtt tttaagaact cctagt
//
LOCUS       DACTGW010000059        19045 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.059, whole genome shotgun sequence.
ACCESSION   DACTGW010000059 DACTGW010000000
VERSION     DACTGW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.059"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     repeat_region   674..1030
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=674..702
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            1269..1457
                     /locus_tag="I9573_001605"
     CDS             1269..1457
                     /locus_tag="I9573_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796231.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     gene            1576..2442
                     /locus_tag="I9573_001606"
     CDS             1576..2442
                     /locus_tag="I9573_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HAT4796232.1"
                     /translation="MKNLIVKEFNGSQIYTFMWKEKSCWIANQIVGLFDYADVSKTIQ
                     DCIKAEDFEIEQEYDVLKGNEFNDFVTTLNVVANNIISNKARSITIFYEDGLYGFLQY
                     TDKPIGVQFRKWLRREVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLK
                     ELLDGAGFDNKSKLLTAKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPA
                     QLAVCEIIKKIDLEENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPTKIAG
                     EKKNYHVVYKIE"
     gene            2870..3064
                     /locus_tag="I9573_001607"
     CDS             2870..3064
                     /locus_tag="I9573_001607"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796233.1"
                     /translation="MEIHNLFWGFILTIWNVNSPISINLVDISLSFILTIWNVNLCNG
                     SVFKIETSSFILTIWNVNQH"
     gene            3373..3900
                     /locus_tag="I9573_001608"
     CDS             3373..3900
                     /locus_tag="I9573_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAT4796234.1"
                     /translation="MREGKRRRGCLFWFILIILFSGVVGAIAGNSTNNESTEKQKEDL
                     TKYIGEEGSIGDLKLTVNSISKASEISVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDDSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     gene            4038..4805
                     /locus_tag="I9573_001609"
     CDS             4038..4805
                     /locus_tag="I9573_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAT4796235.1"
                     /translation="MGLFGKKKADICCICNTEIGVLNIEDGWICNSCFKEYCDALSMT
                     KAPKILRKLDIEKTISSTKKNNELQKIFNTTNNIENYIEFDEDNKKWLVSKKSINDKK
                     TPIIHSYEDIVKFELLENGETVTKGGIGRALAGGILFGEAGAIVGGITGKKTTRKVVD
                     TFKIKITINNIDNPIEYIELINKKTKTNSSAYEKAYKDAHKILSTLSAITQSIKETDN
                     INTKSVADEILKYKNLLDMEAITQDEFNTKKKELLNL"
     gene            4871..7243
                     /locus_tag="I9573_001610"
     CDS             4871..7243
                     /locus_tag="I9573_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAT4796236.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLERLQQRLHNPLN
                     TGGIQASQQSLNNIENILTRIEQKIGRNTNEQENFNNKIKQGSEAGSLLVSKLKSLAG
                     IYIGIRGIESITKAADTIASTKARLNLMNDGLQTTEQLNKMIYLSAQSARASYADTAA
                     QVAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPVTFSQALTMMKNDAYMIFGQTLGKISGALQSVRFSEIVVSIRNVMIAISSNIYDT
                     LNIIKNILNSEFFSNLVQGFTFCAVVITKSLGSIVNTALNIVNIFAQNWSIIKPIVFG
                     VASVFILFRGVLLATKIATIGNVIANVAHAASSSLSALMTNIQAAALMRSNGATLSAT
                     IATWGLNAALLACPITWVVLGILAFVVVVFVAVAAVNKFAGTSLTALGVIVGAVFAAV
                     AAIQNVMIWLLNGCIAVNEGITNGWNQCVYLMKQAIAKGVIFIIEKMASLNNSVNNAG
                     NALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYIKQQIDSL
                     NRWVGDAPEKVKLERMGYKDIGAAFQKGNALGTKWQNAITDKFKDTFDINKMLEDAKK
                     KLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQ
                     ETINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            7260..7949
                     /locus_tag="I9573_001611"
     CDS             7260..7949
                     /locus_tag="I9573_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAT4796237.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKNLSKY
                     TIYKGQVLKLG"
     gene            7942..9834
                     /locus_tag="I9573_001612"
     CDS             7942..9834
                     /locus_tag="I9573_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HAT4796238.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKILAKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSIMKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLNKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSNTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            9848..10108
                     /locus_tag="I9573_001613"
     CDS             9848..10108
                     /locus_tag="I9573_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HAT4796239.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKMGDKLVLIRMQGGQQYLILDRM"
     gene            10113..10532
                     /locus_tag="I9573_001614"
     CDS             10113..10532
                     /locus_tag="I9573_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HAT4796240.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            10533..11582
                     /locus_tag="I9573_001615"
     CDS             10533..11582
                     /locus_tag="I9573_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAT4796241.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATHTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYRDEVNKIQDVGGVKVYPTWDGGGTVKLVIINSNFKV
                     PSEELVNLVQEEIDPIGHQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            11575..12192
                     /locus_tag="I9573_001616"
     CDS             11575..12192
                     /locus_tag="I9573_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HAT4796242.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            12204..13229
                     /locus_tag="I9573_001617"
     CDS             12204..13229
                     /locus_tag="I9573_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAT4796243.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTELKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNINNIELKIDPTIIFATLKDIQDLDAK
                     ISNVNTKIDTTKTELTSNIETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWIDNQEGGFKATVTHGLLTQRITVSIIDATTKDNVVPDFTIIDDNSIEVRSGVKV
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIHEEIGKVAEQLTGINSNIISTV
                     NNDILPI"
     gene            13246..14772
                     /locus_tag="I9573_001618"
     CDS             13246..14772
                     /locus_tag="I9573_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796244.1"
                     /translation="MALSMSYFNLPDKRKYTKNLAFNPFAGGRQNFEWTGGDHGLNGE
                     FKETCLSCTYNGSTLNWGSGNVWVLGEYGQYTFTYNCESMHVDTQQKFPYTSNRIITI
                     KGRPVISGSDTSLGNKRKGFSVDFTVSDDTPNVNLIVRAYLDDKLIQNITPVVQNSTL
                     TATVTDSQLNSLSVDGNHKLKIQLNDGYDNFDRIFTFKKIEKGIDISTSLVTDSQAKF
                     TVTKIYSELTKIECYLDETLKETFTTDLYSEKTINYELIDNAIHTLKIVVTDAENVVE
                     EKVISISKNIMPLQPDATLQDISTKLTEIGQGVRNGKTSIINTLALKNIDASLNNTLV
                     ELSEKIKGGFDSGDASLQDLMNQLTQANNTISQLNTKYKVASGTVTSFADSTKIAYPY
                     LTDNVTKPGSWIKVSNLGFKPNIFFADFDYYDAEYKNNYKLFLFACNGVATQRGVDFS
                     SVTSFIRKSGDEYFHANGWLYSNSEGDVYFNNTGVQIPAYNFDSTQKHTYKWYAIKFI
                     "
     gene            14787..15080
                     /locus_tag="I9573_001619"
     CDS             14787..15080
                     /locus_tag="I9573_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796245.1"
                     /translation="MNVPNRIIYDQTGRTIFETGESCGDVLPHYTITELHYIDIEYGS
                     IDYTRNRVIGINIETKEPILEEIPVYITDEEKRIQELENQLLIAENEKVGGLL"
     gene            15080..15262
                     /locus_tag="I9573_001620"
     CDS             15080..15262
                     /locus_tag="I9573_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796246.1"
                     /translation="MNINNVVVRILAERILSKGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGAEPTI"
     gene            15296..15862
                     /locus_tag="I9573_001621"
     CDS             15296..15862
                     /locus_tag="I9573_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796247.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPTTSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITINS
                     NKTGFSVHSYDTSYESYPFTGYYIACFA"
     gene            15893..16183
                     /locus_tag="I9573_001622"
     CDS             15893..16183
                     /locus_tag="I9573_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796248.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNNSNRKIQDETETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKIEASTKQAHLRIDEIVNRIGGK"
     gene            16187..16444
                     /locus_tag="I9573_001623"
     CDS             16187..16444
                     /locus_tag="I9573_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAT4796249.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            16705..17568
                     /locus_tag="I9573_001624"
     CDS             16705..17568
                     /locus_tag="I9573_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HAT4796250.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            17647..18459
                     /locus_tag="I9573_001625"
     CDS             17647..18459
                     /locus_tag="I9573_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT4796251.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            18553..>19045
                     /locus_tag="I9573_001626"
     CDS             18553..>19045
                     /locus_tag="I9573_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HAT4796252.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLHKNPDVSNPNKIDNLDGIENFRNLEKLDCSSNNLESVDLSKNIKLKYLDISGNKIL
                     NIDL"
BASE COUNT         7704 a         2066 c         3471 g         5804 t
ORIGIN      
        1 ataccacaaa ttagaaaaaa tattgtttat ataaaataaa aaataaaaat ttttattaaa
       61 aaaatgaaaa cttgattata aagcaattaa tttataaaat atatataaat aagtaggtat
      121 ttatttactt gaatttcatt gtttatataa aaaaaatgga aaaatatgta ataattatag
      181 gttataataa ttgtagcaag gataataatc gaaagtgcga agggtgatta ttttcatatt
      241 aaacgccaaa ttccaaataa ggaaggaggt gaaattatat gataggtttt ttattaagca
      301 tactagctgg tgttatatca gcttatattt atgacaaaat aaaaaatcac ccagacgcca
      361 ataagggtga tttaaaaaaa taatattttc acttaacaac tgaaaataat cactctttgt
      421 aggaataaat tatttccttg cttttattat accacaaatt ggtacagata ttcaaaaata
      481 atatttttat gatataataa aaatgtagag attttgcagt gagcgatatt tgtgataaat
      541 tgaagtttaa cagttgcaat ataaggcgtt gagggtgtgt gataaatgtt atcaattgca
      601 ctactcatgg ttcactgcaa atttaagaga gttgtatatg tgtaggtatt gaaaatactc
      661 aacttatttt ggggttttag attaactata tggaatgtaa atgttgtaag aagtatcatt
      721 ctatttttta atctttctgt tttagattaa ctatatggaa tgtaaatttc agtgagaata
      781 agctttattg tcgatgtaac actcgtttta gattaactat atggaatgta aatagtacat
      841 ataatgagtc tttaacatca gttatgaaag gttttagatt aactatatgg aatgtaaatg
      901 attgtacttt agcgtctgca ctagctttgt ctatcgtttt agattaacta tatggaatgt
      961 aaattatttt acagatgaac aattacagtt acttcttgaa tgttttatat taactatgtg
     1021 gattcaaaat taaatagaaa aaagaaagca cttacttaaa tagtaggtgc ttttgttttg
     1081 ctcaaattac ttaattataa agttgctaag tacaactttt tagaattata caatataaaa
     1141 atgcactaaa gtgattgatt ttgctcaagt tggtcggttg agtaaaataa ttagaaaaaa
     1201 atgttaaaaa actcttgaaa agtgtcgcga tacaatgtat aattatatta tcgcgataca
     1261 gaaaagaggt gaaaattatt actgatagca gtagagcaga ttacttcaag cagagacgac
     1321 agaataaaaa aacttttagt gttctactag atagagagaa agtagagaaa attgaagaac
     1381 atttaaaaaa gcagaacaag actaaaacta tttggcttga agaaaagatt aatgaagagc
     1441 tagaaaaaga ggaataaaaa ataagagacg ttctccccga ccaaagattg aacatccctt
     1501 attgacgtat attatataca ctaactatag tatacgtcat tccttgaaaa aattcaatta
     1561 aggagtgtaa tagttatgaa aaatttaata gtaaaagagt tcaatggaag tcaaatttat
     1621 acttttatgt ggaaagaaaa atcttgttgg atagctaatc aaatagttgg attattcgat
     1681 tatgctgatg tatctaaaac aatacaggat tgcataaaag cagaagactt tgagattgaa
     1741 caagagtatg atgtattgaa aggaaatgaa tttaatgatt ttgtaactac tttaaatgta
     1801 gtcgcaaata atataattag taataaagct agaagtataa ctatttttta tgaagatggt
     1861 ttatatggat ttttacaata cacagacaaa cctattggtg tacagtttag gaaatggctt
     1921 agacgagaag ttttaccaag cataagacaa actggtgcat acataactaa caatgctaat
     1981 cccgaaaaac taagagaaaa agcaagtgag attgagaaat tacagttagc ttacaatagt
     2041 acatctatgt taaaagaact attagatggt gcaggctttg acaataaatc caaactatta
     2101 acagctaaaa cattatataa gaaagcagga attgatttac caatagagat aaacgaagaa
     2161 gaacattatt ttgatacaaa gcaaatagca tctaaactga aaatatattc taagagtaat
     2221 aaaccagcac agttggctgt ttgtgaaatt attaaaaaga ttgatttaga agaaaacgaa
     2281 gtcaaaggcg tttgggaaac taatggttct tggactggta ctgtaaataa atatacaaag
     2341 agtgtaatag ataaggttag aaattggata gaggaaaata atagacctac taagattgca
     2401 ggtgaaaaga agaattatca tgtggtttat aagattgagt aaatttataa gattgtatta
     2461 aataatttag tttagttttg gggggattaa tacaatgcat gagaatttac ttgatagtat
     2521 agatattgaa aagagaaaag aagaatttag aattaaactt ttgaaaataa gagaaacaga
     2581 tatagatata tataataaga tagaaagtat agtatataaa ctttcttaga aaaaattaga
     2641 gaaaaataat taaataaata gataaagcac ttggatattt cactgtttca agtgctttat
     2701 atggtaaaaa atggtataat aaaatatagg aattttgcag tgttcgattt ttctactcaa
     2761 gtatagttta acaattggaa tacaaggcat tgagaaggtt tgataagtgt tatcaattgc
     2821 actattaccc gctcactgca aatttgagaa agttatatgt gtgtaagtct tggaaataca
     2881 caatttattt tggggtttta tattaactat atggaatgta aattctccaa tctctatcaa
     2941 cttagttgat atatcactta gttttatatt aactatatgg aatgtaaatc tttgtaatgg
     3001 tagtgtattt aagattgaaa catcaagttt tatattaact atatggaatg taaatcaaca
     3061 ttagtagttg tctttataca catagcatca cgttttatat taactatatg gaatgtaaat
     3121 acagctccca agacatacaa cgaatctgta acatcagtgt tttatattaa ctatgtggta
     3181 tgtaaaagac ttatttacag ctttatttgc taaatcagaa ggttttatat taactatgtg
     3241 gacttaaaat taaaaataat taaaaaacac ttactaatgt aggtgttttt ttattgaaag
     3301 gaagtgatta taatgtaaaa attttgaaga tatagtataa tattcttata aaatataatt
     3361 tagggggata ttatgagaga aggaaagaga agaagaggat gtctattttg gtttattctt
     3421 attattttat tttctggagt tgttggagca atagcaggga acagtactaa taatgaaagc
     3481 actgaaaaac agaaagagga tttaactaaa tatattggtg aagaaggtag tataggagat
     3541 ttaaaattaa ctgttaatag catttcaaaa gctagtgaaa tatcagtagc atctggttat
     3601 ttagcataca ctccagatag tggcaaatat ggtattataa atttaacaat aaaaaatcaa
     3661 actaaggaaa gccaatcttt tatgctaaat tcattcacat taataggacc agatgattca
     3721 aaatatgttc catctttatt gattgatgta ggaaataaat atataactat ggatactgta
     3781 aatcctaatt tagatgtaac aggtaatatt gcatttgaaa ttcctaagga tttactagtt
     3841 tctgattgta aactaaaata tagtggttca gagcaagaat ttatattaaa agaaaaataa
     3901 tatatagatt taaaaatatg aagcatttac tttaaataag taagtgcttt tttaattaaa
     3961 aggaggtgat aataatgtaa aaattttact tatatagtat aattacatta taaaattatt
     4021 gtactggggg gaatcttatg gggttatttg gaaagaaaaa agcagatata tgttgtattt
     4081 gtaatactga gataggagta ctgaatattg aagatggttg gatatgcaat tcttgtttta
     4141 aagaatattg tgatgcactt tctatgacta aagcacctaa aattttaaga aaattagata
     4201 ttgaaaagac tatatcatca actaaaaaaa ataatgaact tcaaaaaata tttaatacaa
     4261 ctaataatat agaaaactat atagaatttg atgaagataa taaaaaatgg cttgtgtcta
     4321 aaaaaagtat aaacgataaa aaaactccta tcattcattc ttatgaagat atcgtgaaat
     4381 ttgaacttct tgaaaatggt gaaactgtaa ctaaaggagg aataggaaga gctttagcgg
     4441 gaggaatttt atttggagaa gcaggagcta tagttggagg gataacaggt aaaaaaacaa
     4501 caagaaaagt tgttgataca tttaaaatta aaataacaat taataatatt gataatccta
     4561 ttgagtatat cgaattaatt aataaaaaaa caaaaactaa ttctagtgct tatgagaaag
     4621 cttacaaaga tgcccataaa attttatcaa cattatctgc tattacacaa agcattaaag
     4681 agacagacaa tataaatact aaatctgtag cagatgagat attaaaatat aagaatcttt
     4741 tggatatgga agctattaca caagatgaat ttaatactaa gaaaaaagaa ttattgaatt
     4801 tgtaatataa taaacactta ctaatgtagg tgttttttta tatggaaatt tatgaaagga
     4861 gagtgagaaa gtggcaacga tacaaacttc aataaagatt ttcgacggaa tgacacctgc
     4921 ttttaggaat atgactacat ctattaatac gacaattaat agccttgaaa ggttacaaca
     4981 aagattgcat aatccgctta acacaggtgg gatacaagca tctcaacaaa gtttaaacaa
     5041 tattgaaaac atattaacca ggatagaaca gaaaattgga agaaatacaa atgaacaaga
     5101 aaactttaat aataagataa agcaaggaag tgaagcaggt tctctattag tatctaaact
     5161 caaaagtttg gctggcatat atattggaat aagaggtata gaaagtatta caaaagcagc
     5221 agatacaatt gcaagtacaa aagcacgttt aaatttaatg aatgatggat tacagacaac
     5281 agaacaactt aataagatga tttatttatc tgctcaaagt gcaagagcta gttatgcaga
     5341 tacggcagca caagtcgcta aacttggaat attagcagga gatgcttttg gaagttcggc
     5401 agaggtgata aagtttacag aacttatgaa taaagctttt gtaattggag gaacatcagc
     5461 aaatgaagct agtgcggcta tgtatcagct tactcaagct atgggtgcag ggaaacttca
     5521 aggtgatgaa tttcgctcta taatggaaaa tgcaccttta ttagccacta aaatagctga
     5581 tgcaatggga aaaactaaag accaattaaa ggaattgtca agtagtggag caataacagc
     5641 agatgttata agaaatgcac tatttaaagc ttctgatgag atagaaaaga aattcgcaag
     5701 tatgccagtc acttttagtc aagcacttac tatgatgaaa aatgacgcct acatgatttt
     5761 tgggcaaacc ctcggaaaga taagtggagc attgcaaagc gttagattta gtgaaattgt
     5821 tgtatctata agaaatgtta tgattgcaat atcttcaaac atttacgata cattaaatat
     5881 tataaaaaat attttaaata gtgaattctt ttcaaactta gtgcaaggat ttacattttg
     5941 tgcggtagta ataactaaaa gcttaggttc tattgtaaat actgcattaa atatagttaa
     6001 tatatttgca caaaattgga gtataataaa acctattgta tttggtgtag cttctgtttt
     6061 tatattgttt agaggggttt tgctagcgac taaaatagct acaataggta acgtaattgc
     6121 gaatgttgct cacgctgctt cctcttcact atcagcactt atgactaata tacaagcagc
     6181 agcactgatg cgttcgaatg gagctacttt atcagcaaca atagccactt ggggtttaaa
     6241 tgcagcttta ttagcttgtc caatcacatg ggtagtatta ggtattctag catttgtagt
     6301 agtagttttt gttgcagtag cagcagtaaa caaattcgca ggtacaagtt taactgcctt
     6361 aggagtaatt gtgggtgctg tatttgcagc agtggcagca atacaaaatg ttatgatttg
     6421 gttattaaat ggctgtatag ctgtaaatga aggcattaca aatggttgga atcaatgtgt
     6481 atatctaatg aaacaagcta ttgcaaaagg tgtaatcttt ataatcgaga aaatggcatc
     6541 tctaaataat tctgtaaata atgctggaaa tgcacttggg aaagctttca tagatggggc
     6601 aaatatcgca atacgaggtg taaacaaatt aattgaccta ataaataaaa taccagggat
     6661 aaatattggt aaagtaggag aggcaacgtt tacgccagtt aaggcagata atagttacat
     6721 caaacaacag attgatagct taaacagatg ggtaggagac gcaccagaga aagtaaaatt
     6781 ggagcgaatg ggatacaaag atattggagc agcatttcaa aaaggaaatg cacttggaac
     6841 taaatggcaa aatgctataa ctgataaatt taaagatact tttgacatta ataagatgct
     6901 agaagatgca aagaaaaaat taggattaga cgatttgtgg aataaacaaa atcctttaaa
     6961 caaccttggt ggatttggtg gagatttagg aaaaaatgta aaggacacgg caggcaatac
     7021 tgcaaagatg gcaaagacta tggataaaag tcaagaggac ttaaaatact taagagatat
     7081 agcagagcaa gaaacaatca atcgattcac aggagtaaac attaaaatag atatgaacaa
     7141 tacaaacaac ataagtaaag atacagatgt tgatggaata gttaatgtct taactgaaaa
     7201 actgaacgat gctatggttg tatcagcgga aggaatagtt taggaaggag agtgagaaaa
     7261 tggcttatga tttttaccta gatggagtac aactaccaat cgcaccaccc aagcttgaag
     7321 tcaaagtgac aaacaagaac aagacagttg atttgataaa tactggagaa gtaaacatat
     7381 taaaaaaaga aggattatct gaaataagtt ttgaagcaga atttacacat aataaactac
     7441 ctttttgtag aggtcaattt agagatgttc aattcttttt aagtaagcta gaattactaa
     7501 aaactgattg taagccattt caatttattg tatctcgtga gttaggtaac aaggtcttat
     7561 ttaacactaa tataaaagta tctcttgaag agtatgctat ttcagaagat gcagataatg
     7621 gctcagatac aaaagttgca ataaaattaa agcaatatag agattactca actaaaaagt
     7681 tagttcttgc ccctcctaaa aatgagactg gtagacctaa tgtaaagata gagccaaaac
     7741 gagttgattc agtcaatgcc acaaacacta aaacatatac agtaaaagca ggggatagcc
     7801 tttggtcaat ttgtcagaaa caacttggta atggttcatt atataagaaa gtatacgaac
     7861 taaataaatc tatgatggat aaggcaaata agggcaaaaa cttaagtaaa tacactattt
     7921 ataaagggca ggtgttaaaa cttggttgat gaattagtgt tagcaaatga tagagatgta
     7981 agattagtaa tagctcattg ggaagatttc tacgaacctg ctgtcattga tggtatcaca
     8041 tgggagatag aaagacgagg tacaccttct aagttagagt ttacaatagt catggatgat
     8101 atactagagt tttgcgaagg aaattctgta aggctgtatt ataaaggaat aggcatattt
     8161 tatggatata tatttcaaaa gaaaagagat aaagaaaatc acattaaaat tgttgcttac
     8221 gaccagttga gatattttaa gaataaagat acttatgtat atagtaataa aactgcaagt
     8281 gaacttgtaa aaatcttggc taaagatttt aatttaaaat acaatgtcat agaagatact
     8341 aagtataaaa tatctagagt cgaagaaaat aaaacactct ttgacatgat actaacagca
     8401 ctagatgata ctttaagaga gaaaaaagaa atgtatacct tgtatgatga ttttggaaga
     8461 ataacattaa agaatgttgc atcaatgaaa ctagatactg tcatgaacaa tgatgtaatt
     8521 gaggattttg actataattc atcaatagat agtgatactt acacaaagat taaacttgta
     8581 agagacaacg aggagtcagg aaaaagggat gtatatattg ctcaagactc agctcacatg
     8641 agaagttggg gaatacttca aatgtttgat acagtagaca aaaacatgag tgaagcagag
     8701 ataaaacaaa agtgtgatat acttctaaaa ctatataata agaaaactaa gtcattaagt
     8761 ttaaaaaatg cacttggaga tattagagtg agagcaggtt gtttagtacc tgttttttta
     8821 aatctaggag atattgaatt gcaaaattat atgttagttg agaaagtaaa acatacattt
     8881 gaaaataatt cacatttcat ggatttgacc ttggttgatg gagacgaatt tgcttcatat
     8941 tcttcaagct catatagtag tggaaatact aataataaag atgaaaagaa aaatggtcct
     9001 gcacaaagta ttatgaaaaa aaatacaggt aaaaaagttc ctgctatatt tactgcatat
     9061 tatccaggaa acaatgcaat ggaaggtgga aaaacagatt gcaatggaaa gccacttgat
     9121 gtaaaatcaa gaactgttgc tggtccaatg aatcgagaag gagttaagaa aacttggtat
     9181 actgatgatt ttctaaataa acatccagtt tttgaatatg gagataaagt aaaaattata
     9241 cttcctggta ctgcctatga caacaaagta tatacagtta aagataatgg aggaagaata
     9301 tatgttgaaa caaacggaac atatcatata gatatactat tagctaatgc tagtgaatgt
     9361 aaaaaatttg gtagaaagaa tggctatata attataggtg gagatgaaga acaaacatat
     9421 caagttgaag gtaataacca aagtaataca aataacaatt ctaaagaaga taaattaatt
     9481 agtatagcaa aaagtaaact gggttgtaat tatgtgtatg gagcagaagg tcctaataat
     9541 tttgattgca gtgggtttac tcaatggtgt tataaacaaa taggtataaa aattcctcgt
     9601 actgcttctg cacaaagtaa agcaggaaaa gcagtagatt taaaagatag aagcaagtgg
     9661 aaagcaggag acttattatg tagaattggt ggaggaagta gtaatcatgt tgtaatgtac
     9721 attggaaaca atcaaataat tcattcacca caaacaggag atgtggtaaa aatagagtct
     9781 gttaattctt atagaaaagg aaaagcatac acacatgtga gaagatttat ataagtgagg
     9841 tggcaatatg agccaagatt tattacagat aataaaaaaa gctgcaatgg atgcagtaga
     9901 aacaagcaac ccaatgaggg ttgtatttgg aacaatagaa agtattagtc ctctaagagt
     9961 taagatagaa caaaaactat ctattggtga aatttttcta atacaaacag atacatttaa
    10021 aagatataca gataaaaaaa tgggagataa attagtctta attcgtatgc aaggagggca
    10081 acaatacttg attttagata ggatgtgata aagtgttacc aagtgataat ttagattatg
    10141 atattgaaga tgtatcaata attaattttg atgtaagaca agagccaagt aagacattta
    10201 aacttcatat agaaaagtct aagattgatg gtatttgtga tgatgtagaa gcattaaaac
    10261 aaaccatttt tttaatttta aatactgaaa ggtatgagca tcttatttat tctagaaatt
    10321 atggtgttga attaaatgat ttaattggag aacctatttc atatgtaata cccgaacttg
    10381 aaagaaggat aacagaagca ctaattcaag atgataggat tgaaaatata gataattttg
    10441 agtttcaaaa tataaagggt aaagtacaat gtagattttc agttcataca aaatatggaa
    10501 atataaaagc agagaaggtg gtgagtgtat aattgtttga gttaatgaca tttgaaaata
    10561 taattaaaag aatgttagat agtgtaccag atacttttga taaaagggaa ggttctataa
    10621 tatataatgc tcttgctcct gttgctatag aacttacaga aacatacatt gctatggatg
    10681 aattactaga ccaaacattc gtagatactg ctagttatta ttatttagag aagagatgta
    10741 aagaaagggg aataacacca cttgaagcca ctcatacgat tgctaaagga gtttttaaca
    10801 tagatattcc acttgattct aggtttaatc taggagaata taactatgta gcaattgaga
    10861 gaatatctga aaaaacatat aaaatgaaat gtgaaactac tggacctgta tttgagttag
    10921 gaaaactaat acctattgaa tatatagatg gtcttgaaac tgctgaacta actgaaatct
    10981 tgataaatgg agaggatgaa gagtcagaag atagtttaag acaaagatat tatgatagcc
    11041 taaattcaca gagttttggc gggaatatac aaaactatag ggatgaagtt aacaaaatac
    11101 aagatgttgg aggagttaaa gtttatccaa cttgggatgg aggtgggact gttaagttag
    11161 taataattaa ctctaatttt aaagtgccaa gtgaagaatt agtaaactta gttcaagaag
    11221 aaattgaccc aattggacac caaggagaag gcttaggatt agcaccgatt ggacaccgag
    11281 tcacagttga aggagttaca agtacaacta taaacatatc agcagaaata acatacaaaa
    11341 atggctacac ttgggagaat ataaaatcaa ttgcagaaga agcaatagac gactatttaa
    11401 atgaacttaa catgagttgg gaagatgaag aaaacttaat agtccgtata tctcaaattg
    11461 aaactagatt acttagtatt gatggagtat tagacattgc aaatacaatg ataaatgatg
    11521 ttaaatctaa tctaacaata gatagtaaca gcatagtagt gagaggtgag gtagttggat
    11581 aaagagatta atctaataaa ttacttgcca caaattctac aagataaaga agaatatata
    11641 aaagtattta atgtagaaaa taaagaaata aaaacactac atgataaatt aaaggaccta
    11701 tcaaatgacc agtttttaga ggacctaact ataagtggta taaaaagatg ggaaaagata
    11761 atgtctataa ctcctaaaag taatgagagt ttagaagata gaaggtttag gatttttagt
    11821 aaatatataa gtaaattacc ttactcagag agatttttaa ggaactggct agataatgta
    11881 gttggagaag gcaattatga attaactatt aataatgcta cttataacat acaccttgag
    11941 agtgatgcta gaaatcaaga ttggtttgag gaagttcatt cttttgtaag taatattaag
    12001 ccatgtaata tgactttaga ttacactaga gtgcttataa gcaaagacaa ttatatgaat
    12061 tttggtataa caaccctaat gggtcaagaa ataactatat acccttggag tccaccagat
    12121 atagaaactt atggagaaat tgatgtatta actggcaatg gagttggata ccaagagata
    12181 acaatatttt aggaggtgat atattggcta tagataaaag ttattacact ataattacag
    12241 atgtaggaaa agcaaagata gcaaatgcaa gtgtcacagg taataaagta gggtttgtaa
    12301 aaattcaact tggtgatggt ggaggaagtg agtatactcc tactgaaagt cagacagaac
    12361 ttaaaaatgt ggtatgggaa ggcaacatcg gaaatacaac cacagatgaa actgcgccaa
    12421 actgcataat attagagagt ttaataccat caagtgtagg tggatttatg ataagagaaa
    12481 taggatattt agatgatgaa aataatctaa ttgcaatctc taaatataaa gaatgttata
    12541 aaccttctat agaacaagga gcagttgttg atatgaaggt taaaactgtg cttattgtat
    12601 ctaatataaa taatatagaa cttaaaattg accctacaat aatttttgct acgctcaaag
    12661 atatacagga tttagatgct aaaataagca atgtaaacac taaaattgat acaactaaaa
    12721 cagagttaac aagcaacata gaaactgcta agacagagtt aaacactaga attgacacag
    12781 aaaatgagaa acaaaatatt aaaattgacc agctaatagc aggtggttca aatgttgcat
    12841 ctactcaaac aataacaatt gatgattgga tagataatca agaaggtgga ttcaaagcaa
    12901 ctgtaacaca tggtttatta acacagagaa taactgtaag tattatagat gctactacaa
    12961 aagataatgt agtacccgat ttcacaatta tagacgataa ttctattgag gttagaagtg
    13021 gagtaaaagt tgagttaaat gtttatgtga ttaatgggaa tgcagagact cattttatta
    13081 atgcgactgt agatgataat agagtgtctg aaatgactac ttattcatca aaaaagatac
    13141 atgaagaaat tggtaaggta gcagagcaac tgacaggaat taatagtaat attatatcga
    13201 ctgtaaataa cgacatacta cctatataga aaggagcgtg aaaaaatggc gttatcaatg
    13261 agttatttta atttacctga caaaagaaaa tatactaaaa atcttgcatt taatccattt
    13321 gcaggtggaa ggcaaaattt tgagtggact ggtggtgacc atggactaaa tggtgagttt
    13381 aaagaaactt gcttaagttg cacttacaat ggttcaacat taaattgggg ttcaggaaat
    13441 gtttgggtct tgggggaata tggtcaatat acatttactt ataattgtga atcaatgcat
    13501 gtggatacac aacaaaaatt tccgtatact tctaatagaa taataactat aaaaggtaga
    13561 cctgtaatat ctggttcaga tacttcactt gggaataaaa gaaaaggatt tagtgtagat
    13621 tttactgtaa gtgatgatac tccaaatgtt aatttaatag tacgtgctta tcttgatgat
    13681 aaattaatac aaaacattac tcctgttgta caaaattcaa ctttaacagc aacagtaaca
    13741 gatagtcaac taaactcttt atccgtagat ggaaatcaca aactaaaaat acaattaaat
    13801 gatggatatg ataattttga tagaattttt acttttaaaa aaatagaaaa gggaattgat
    13861 atatcaacat cattagtaac tgatagccaa gctaagttta ctgtaacaaa aatatacagt
    13921 gaattgacaa aaatagagtg ttatttagat gaaacattaa aagagacttt tactacagac
    13981 ttgtattcag aaaagactat aaactatgaa cttatagaca atgcaataca cacattaaaa
    14041 atagttgtaa ctgatgcaga aaatgttgta gaagaaaaag ttataagtat aagtaagaat
    14101 ataatgccat tgcaacctga tgcaacatta caagatatat caactaagtt aacagagatt
    14161 gggcaaggcg ttagaaatgg taaaacaagc attataaata ctttagcatt aaagaatata
    14221 gatgcaagtt taaataatac actcgttgag ttatcagaga aaattaaggg aggttttgat
    14281 agtggagacg ctagtttaca agatttgatg aatcaattaa cacaagctaa taatactata
    14341 tcgcagttaa atactaagta taaagttgct agtggtacag ttacttcttt tgcggatagt
    14401 actaaaattg cttatccata tctaaccgac aatgttacta aacctggttc ttggattaaa
    14461 gttagtaatt taggctttaa acctaatatt ttctttgctg attttgatta ctatgatgct
    14521 gaatataaaa ataattataa acttttcctt ttcgcttgta atggtgttgc cacacaaaga
    14581 ggtgttgatt tttcaagtgt tacatctttt atcagaaaaa gtggtgatga gtattttcat
    14641 gctaatggat ggctatatag taactcagag ggggatgtct attttaataa tactggcgtt
    14701 cagattccag cttataactt tgattcaact caaaaacata cttataaatg gtatgctatt
    14761 aaatttattt aaaaggagga aaaaacatga atgttccaaa ccgaataata tatgaccaaa
    14821 caggtagaac aatctttgaa acaggcgaat cctgtggaga tgtattgcca cattatacaa
    14881 taacggaatt gcattatata gatattgaat atggaagtat agattataca aggaatagag
    14941 ttataggtat aaatatagaa acaaaagaac cgattttaga agaaatacca gtatatataa
    15001 cagatgagga aaagagaata caagagttag aaaatcaatt attaattgca gaaaatgaga
    15061 aagtaggagg attattataa tgaatataaa taatgttgta gtaagaatat tagcagaaag
    15121 aatattaagt aagggattaa atcctctaaa aaatagagaa tttcagttag atgacgtgac
    15181 taacacagag tacagaaaag ctgtagagga ttatattata aaaaatagtg gagtagtaga
    15241 aggagcagaa ccaactatat agagggttct ttttttattg aaagaaggtg actaaatgac
    15301 ttttaaagag ttggttaata aagttagaaa tcttgtatta gaagcaaaga atgtaactat
    15361 agaagataca gaaagtaaat ttacaagtga aaatgtagaa ggagcattga aagaatgtat
    15421 agatagagca gatgaggctt ttcaagaagc cgatagtgga aaaacacttt tatcaactgc
    15481 tatcggctct cctactacat cagaacaaac atttcaagat tatgcgaact atattacagg
    15541 atttaagagc aatataagca atttagaaac tcaattgaaa agcaaatatt ctattagata
    15601 tggtcctatt gatggatatg atggtaatcc tttttctgct aattttggca agagtgcaag
    15661 ttaccttatt gtctatgttt actttagaag aagtgtatat tattataatc ccagtggtag
    15721 ttctttagga agtaatacag gaggttctga acgtgcatgg attactataa acagcaataa
    15781 aactggtttt tcagttcatt catatgatac tagttatgag tcatatcctt ttacaggtta
    15841 ttatattgct tgtttcgcat aataaattat attattaaaa ctaggaggat gtatggaaga
    15901 aattagcata aatctactat gtgcagtagc aggagttgta atatcctact tagcatttag
    15961 aaataactca aacagaaaga tacaagatga aacagaaaca actacaaaat tagaacaaca
    16021 aataactttt ttatgtgaga atgtaagaga tataaagcat gatgtagcaa agtttaatac
    16081 tagtttctta gatattagtg aacgagttgc aaaaatagaa gcaagtacaa aacaagcaca
    16141 tcttagaatt gatgaaattg taaatagaat tggaggaaaa taagagatgg ataatttaat
    16201 aagttttata ccagagcagt tgctaatttt agtagctgct ctctctatta taggtaaagg
    16261 ttgtaaaaaa tataaacaat tagataataa atacattcca gtagtgttac tgatacttgg
    16321 aataggtttc tcaatatgga tgctaggatt aaatcctgtt gcagtcttac aaggtgtaat
    16381 ttgttgggga gttgcaatag gtataaatca aacttacaaa cagttgaagg atggtgaaaa
    16441 gtaatgaaat taacaaaaat attattactc acaaaaattt aaaaaatttc attaaataga
    16501 gtatactttt atagctaata agtatataat aatagtacag aactcgtaaa gcatttatat
    16561 gctcaaatat acgggacatt gatttttgaa ggaacccgcc aagcttcttt tatagctcaa
    16621 ataggcggga cagataaatt tacccactaa tattaattgg tgggttttat tatataaaag
    16681 tatatctatg aaaaggtgaa aacaatggtt gaagtaaaag aagaaaaaac atttgatgaa
    16741 caaatagata ttttaaaaag tagaggatta ataataaatg ataaagaaga tgctaaattt
    16801 gtattaagta atgtcaatta ttataggttt acagcatatc ttctaagttt taagaatgat
    16861 gatggctcat ataaagaagg aactacgttt gaagaagttt atgatatata taggtttaat
    16921 aaggaattta ggatattatt aacagatttg ttagggagca tagaaatagc atttaggaca
    16981 tacattgcat atacattagc aattaaacat ggtgcttgtg gatatctaga aagggagagt
    17041 ttcaaagatg aaaaattcta tattaatttt ttgacagcat tagagagaga aaaaagtaat
    17101 aattcagata agctttttat tatacaccat aaagaaaaat atgaaggaaa acttcccatt
    17161 tgggttgcaa cggaaataat gacttttggt atgttgtcaa aattatattc aaacatgttg
    17221 ccagaagata ctagatacat aaaaaataat ttgtgtagag tgaatacttt attagttaaa
    17281 tcttggttac aatcattaac acaggtcaga aatcaatgcg ctcattatgg tagaatatat
    17341 aataataatt tccgtattat aacaataaaa aacgaatata aaaagtataa cttggataat
    17401 aaaaagatat tttcttatat acttgctatg aagcatttga ctatggataa attaatttgg
    17461 aatagttttt ttataaaact tcaaaagtta attaatgatt ataataattc tatagactta
    17521 aagcttattg gttttcctaa taattggata gagatattgg ctaaataaaa tagttacttt
    17581 aagaagttta taaacactta ctatatgtaa gtgttttttt attgaaaaga aggaggaaaa
    17641 taaataatga aaatatgtat tacagtagga cacagtattt taaaaagtgg tgcatgcact
    17701 tctgctgatg gagtagttaa cgaataccaa tacaataaat ctcttgcacc agtattagca
    17761 gatacattta gaaaagaggg tcataaggca gatgtaataa tatgtcctga aaagcagttt
    17821 aaaactaaag cagaagaaaa gacttataaa atacctagag ttaatagtgg aggatatgat
    17881 ttacttatag aactacatct aaatgcaagt gatggtcaag gaaaaggttc agaagttcta
    17941 tattatagta ataaaggttt agagtatgca actagaatat gtaataagct aggtacagta
    18001 tttagaaata gaagagctaa attagataaa ggattatata tcttaaatag ttcaaatcct
    18061 acagcagtat taattgaaag tttcttctgt gataataaag aagattatga gaaagctaag
    18121 aaactaggtc atgaaggtat tgctaagtta attgtagaag gtgtattaaa taaaaatata
    18181 aataatgagg gagttaaaca gatgtacaaa catacaattg tttatgatgg agaagttgac
    18241 aaaatctctg caactgtagt tggttggggt tataatgatg ggaaaatact gatatgtgat
    18301 ataaaagatt acgtgccagg tcagacgcaa aatctttatg tcattggtgg tgcagcatgt
    18361 gagaagattg gttctatgac taaagaaaaa tttactatga taaagggtaa tgatagattt
    18421 gatacacttt ataaggcatt agattttatt aatagataga ttatataaaa tattttagta
    18481 tttcttgaaa atcataagta tggtaatatt tagataaata ttatttatta ttaataggta
    18541 tggggggaga ttatgatatt taagaagtat ataaaatcat tactgatagt tgttagtgtt
    18601 acattgataa tgaacaaatc aatattattt gtaaatgcat tagatttaga gagtaacaat
    18661 aacataaaat cttataaaag aaagaatgta aaggaaattg atataagcaa taaatttgtt
    18721 gatgaatcct ttagaagaga tattgttgaa aaggtattag ataataggga tgatccatta
    18781 acaaatgaag ttgcatcaaa agataactat aaaatatatg aaagtgattt aaataaaatt
    18841 gagagttgct tagagttaga tttacataaa aatccagatg tttctaatcc aaacaaaata
    18901 gataatttag atgggataga aaattttaga aatctagaaa agcttgattg tagttcaaat
    18961 aatctagaaa gtgtagattt aagtaaaaat ataaaattaa aatatctaga tattagtgga
    19021 aataaaatat taaacattga tttaa
//
LOCUS       DACTGW010000060        18967 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.060, whole genome shotgun sequence.
ACCESSION   DACTGW010000060 DACTGW010000000
VERSION     DACTGW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.060"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            459..1325
                     /locus_tag="I9573_001627"
     CDS             459..1325
                     /locus_tag="I9573_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAT4796253.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            1567..2376
                     /locus_tag="I9573_001628"
     CDS             1567..2376
                     /locus_tag="I9573_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796254.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKKGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGQGSYTFFVDEGT
                     AMEYIGEFKEDKKNGKGNFVFSNGNEYIGHFEEDMMNGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            2519..3583
                     /locus_tag="I9573_001629"
     CDS             2519..3583
                     /locus_tag="I9573_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAT4796255.1"
                     /translation="MKRLIALVLVILIVLFYSPELIEEGYDLKEKFFQSEIWNEFLDK
                     ININNNSKKDKKEQNQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     KLYPDDREELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HINKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            3611..4330
                     /locus_tag="I9573_001630"
     CDS             3611..4330
                     /locus_tag="I9573_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAT4796256.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVKNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            4354..4938
                     /locus_tag="I9573_001631"
     CDS             4354..4938
                     /locus_tag="I9573_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HAT4796257.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGYVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIIDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLENFITEARERVHM"
     gene            4940..6289
                     /gene="pabB"
                     /locus_tag="I9573_001632"
     CDS             4940..6289
                     /gene="pabB"
                     /locus_tag="I9573_001632"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HAT4796258.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            6279..7022
                     /locus_tag="I9573_001633"
     CDS             6279..7022
                     /locus_tag="I9573_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAT4796259.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            7056..7757
                     /locus_tag="I9573_001634"
     CDS             7056..7757
                     /locus_tag="I9573_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796260.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISNIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELASKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            7783..8355
                     /gene="folE"
                     /locus_tag="I9573_001635"
     CDS             7783..8355
                     /gene="folE"
                     /locus_tag="I9573_001635"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAT4796261.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            8421..9212
                     /gene="folP"
                     /locus_tag="I9573_001636"
     CDS             8421..9212
                     /gene="folP"
                     /locus_tag="I9573_001636"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAT4796262.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELEIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            9223..9588
                     /gene="folB"
                     /locus_tag="I9573_001637"
     CDS             9223..9588
                     /gene="folB"
                     /locus_tag="I9573_001637"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HAT4796263.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            9581..10087
                     /gene="folK"
                     /locus_tag="I9573_001638"
     CDS             9581..10087
                     /gene="folK"
                     /locus_tag="I9573_001638"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAT4796264.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVASTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTVKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            10068..10853
                     /gene="aroF"
                     /locus_tag="I9573_001639"
     CDS             10068..10853
                     /gene="aroF"
                     /locus_tag="I9573_001639"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAT4796265.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLKILKAVKDEVGMAVITELMDVRD
                     IDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            11108..12898
                     /locus_tag="I9573_001640"
     CDS             11108..12898
                     /locus_tag="I9573_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAT4796266.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLDCGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            12916..14082
                     /gene="rpoD"
                     /locus_tag="I9573_001641"
     CDS             12916..14082
                     /gene="rpoD"
                     /locus_tag="I9573_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAT4796267.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            14247..14936
                     /locus_tag="I9573_001642"
     CDS             14247..14936
                     /locus_tag="I9573_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT4796268.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            14950..16047
                     /locus_tag="I9573_001643"
     CDS             14950..16047
                     /locus_tag="I9573_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAT4796269.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            16109..16681
                     /locus_tag="I9573_001644"
     CDS             16109..16681
                     /locus_tag="I9573_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT4796270.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEVLEEMNIEVDIFRVSDEVAKT
                     LPQYYLINYKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(16741..17208)
                     /locus_tag="I9573_001645"
     CDS             complement(16741..17208)
                     /locus_tag="I9573_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HAT4796271.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(17594..18070)
                     /locus_tag="I9573_001646"
     CDS             complement(17594..18070)
                     /locus_tag="I9573_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAT4796272.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLRVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(18190..>18967)
                     /locus_tag="I9573_001647"
     CDS             complement(18190..>18967)
                     /locus_tag="I9573_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HAT4796273.1"
                     /translation="AKKLKSEIYSKLGHIDVDSKLIVDMDFINKKNKIIKKFKIIYLI
                     PILFTLGMSIFIAFNYNKLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLL
                     YITSIGSIKSRIKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTK
                     LSTAMNITTLLVIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVN
                     KRFGIGWTINFGNPLGKILYIAIALLLIFSLFSLIKSLLL"
BASE COUNT         7737 a         1912 c         3459 g         5859 t
ORIGIN      
        1 aataaaaact gaacgaaaaa aatgcaaata atgaaataaa aaaatcatgt actgaaatta
       61 taaaaagaaa aatttcattt aggaattaat tataaagaga taattactaa acttgaattt
      121 gctaaaataa aataaattac tgcgaaaata gatatttaga aaaaaatact ataaatttac
      181 tatattgcaa gttaaataat aagcagtatc atattatgct agaaaaagtt tcatttaagg
      241 cataaatgag atgatttgaa cgtttttaaa gattagaata taaaagattg cactagacat
      301 atatgaaaaa gataatataa tagatgatgt aatagatgac agttcaagaa attataaaaa
      361 aaatttattt gaaatattat ctattacatc aggaataata tatgtaagag taagctttta
      421 aaattctaaa gatttagaaa gattgaaggg agaaagaaat gagtgaagtt aagaataccc
      481 tacaaaaaca tccagtttta agatattcaa caatgctatt tgggatgttg atgacatcta
      541 taggaataaa tgggttctta agaccagcac atctgcttag tggaggtgct acaggtatag
      601 caacatctat aaactatttg acaaatataa atgtaggttt attaacattt ttaatcaata
      661 ttccaatatt tatcttaggg ttcatatacc ttgaaaaaga gttttgtatt tcaagtttgg
      721 taaatatgat tgtattttca ttattattag gagcaactca ggaaataagt aatattatac
      781 ctatacatga tatattgctt caaagtgtat atggtggtat attatctggt ttaggagtcg
      841 gagttgtatt tagaactagg tcttctcaag gtggtacaga tataatagct gcaatactaa
      901 aaattaagaa gaatatagaa atgaaagaca ctgctttagc aataaatggc ttgatagtac
      961 ttacaggaag ttttttattt gggttggatt tagcattata cactcttata ggtctatttc
     1021 taaatgcgta ttcaatgagt tttattaaag atgcaatgaa ttaccaaaaa tctgttatgg
     1081 taatgtcaaa tgaagtcgat ttaatagcag aagatataat gaagagttta gttagaggtg
     1141 taactttttt agatgcagaa ggtgcataca ctcatcaaaa aaagaagatt atatatacaa
     1201 tagtttcatc aaatgagata ccaaagataa aagatatagc tttgaagtat gataaaaagg
     1261 cctttatatc tgtaaatgat gttacagaag taaaaggaag agggtttaaa gcaaaagatt
     1321 tatagaaaat tttacaagct gggagtttat aatgtaaatt tgtatattat atctctcagc
     1381 ttatttaaat ttagtatatg aaaataaagg ttttatcaat gatttgtgta tcactttaaa
     1441 agatatcatt ttaaaattaa aattgaaaaa attactcaat atgtagaaaa cataaccact
     1501 attaggatat aataaattta atagatttag taaaatatct aggaataacg ttggaggaat
     1561 agcattatga atttatctaa agaatatgtt aatttgtatg ataattattt gaaatatata
     1621 aatgaagtaa acaaagagat tagaagtata aagaacatga agaaacgtgt tttaaaaaag
     1681 ggcgattttg ctccagaaaa tgaagtatta gaattggaag gaaaaacaat acgcgatata
     1741 gatgatgtcg atacaaaaaa accaaaaaat aaaatatata aattttcaaa tggggatgtg
     1801 tatgtaggaa aatttttaga aggaaagatg gaagggcaag gatcatatac tttttttgtt
     1861 gatgaaggaa cggctatgga gtacatagga gagtttaaag aagacaaaaa aaacggaaaa
     1921 ggaaactttg tattttcaaa tggaaatgaa tatataggcc attttgagga agatatgatg
     1981 aacggtattg gaaatatgtt atataatagc aaagatgaat atattggaac ttggaaaaat
     2041 ggaaaaaaag atggaaaagg tatttataag tggagtgatg gatgtatata tgcaggtgaa
     2101 tttaaaggtg gaaagatgga agggtatggt atttgctata atagtaaagg ggaagtactt
     2161 tatgaaggag aatggaagaa taatttgatt catggtaaag gaacttatat atgggaaaaa
     2221 ggtaaaaagt atataggtga atttatgcat ggtaaaaaac atggacaagg aactttttat
     2281 ctaaataatg aattggttta tgaagggact tggaaatttg ataagccatc catcttcgac
     2341 agaacattag atgaaatatt ttcattaaga ctatagcttt atctattaat agataaagct
     2401 tttttctaat acttactgta aataagtatg aatgatattg attagtatac actatcaata
     2461 ttaactcaat aaatgtgata ttaaaaattt ttgattgatt ttaagaagga gggatatatt
     2521 gaagagatta attgctttag tgttagtaat acttattgtt ttattttata gccctgaatt
     2581 gatagaagaa ggatatgatt taaaagaaaa attttttcaa agtgaaatat ggaatgaatt
     2641 tttagacaaa ataaatataa ataataattc taagaaagat aaaaaagaac aaaatcaaaa
     2701 tttacaatca aataatactg aaggggagaa cttaggagag tcagatataa agaattttga
     2761 aaaaataagc ttaggagata atttaagcta tgttttatca agtattggta aacctggtag
     2821 aattgatatt agtgaatatg gttttgattg gtatgtatat aatcaatatg gaaaagaatt
     2881 tgcaatggtc ggacttgaaa atgatgaagt agtagcactt tatagtaatt ctattaattc
     2941 atgtgaaaat caagatataa aactaaatca agatagacaa acagtgagaa ctaagattac
     3001 accattaaaa tataagagaa aaggaaatac cagatatata ataaactcag aaaatcaata
     3061 tgatataata agtaaagaag gtaagtatat tacaattttc tacgatattc atgaagaaaa
     3121 tagagtttgc tcatatttaa taattgataa aagtactgaa gatgagttta aaaaattata
     3181 tccagatgat agagaagaac ttaaaaaatg ttttgaactt gaagttatag accttgtaaa
     3241 tagtgttaga aatcaaagag gattaaactc attgagatac agtgaacagg caactttgag
     3301 ttcaagaaaa catagtgaag atatgagaga taataatttt tttgaccata taaataagaa
     3361 gaatgaaact ccatttgaca gaatgaaaag agaaggtata gtatatactt cagctggaga
     3421 aaacatagca gcaggacaga ttaatgcaat atatgcgcat gaagcttgga tgaactctga
     3481 aggacataga aaaaatattt tggggaacta taataatatt ggagttggag taatatttgg
     3541 aggaagctat aaaacatatt acactcaaaa tttttataaa taactaaaat atttgaaagg
     3601 aagttaaaaa atgacatcat ggaaaaaaaa gacagtatat aagtgcttaa tagcagtagt
     3661 cttgttttgt ggaatagtgt taatatctaa ttttagtaaa gtttctgctt tgatgatgga
     3721 tactaatgga aatgttttga ttaaacatgg ttcaagggaa aaaaagctaa tagcaattac
     3781 ctttgatgat ggtcctcatc ctaaagaaac tagtcaagtt ttagatgtat taaagaaata
     3841 caatgtaaaa gctacttttt ttatagctgg aaaacatgca aaatggtata aagaaccatt
     3901 agtaagagca agtaaagaag gtcacgagat aggaaatcat actttcaatc atcctgatat
     3961 aagtaattta agttctagtc agatagaaga ggagattgtt aagtgtgaag acattttgaa
     4021 agaagtaact ggaaaaaaac ctacattatt tagaccacca tttggaagtt atagagaaaa
     4081 agacttaata gaaattgcaa aaaagcatga ctataaagtt gttttatgga caggtgtaga
     4141 tgtgaaagat tggaaaaatc caggagctaa tagcattgca gataaaataa ttaataaagt
     4201 aaaaaatgga gatataatac ttttgcatga ttatgcaact aatgatacag ttgaagcttt
     4261 agatatgttt attccaaaaa tgattgaaaa aggttttaaa tttgtaacag tttctgagtt
     4321 gataaaataa tttttagggg ggagatttac agtatgattc ttatgataga caattatgat
     4381 tcttttgtat acaacctagt ccaatatata gaagaattag gggaagcagt tgtagtaaaa
     4441 agaaataatg aaatcaaaat aagtgacatt gaagaactaa atccagaggt aattgtgtta
     4501 tctccaggac cgtgttctcc aaaagaagct ggtatttgca tagatattgt tgagcatttt
     4561 aaggggaaaa aacctatact aggaatttgt ttaggtcacc aaactattgg atatgttttt
     4621 ggaggagata ttataaaagc acaacagcct gtgcatggaa aagtatatag tatcaaccat
     4681 actaataaag gagtttttag gggacttaaa aatcctttaa atgtcactag gtatcattca
     4741 ctaataattg attccaatac agttccaaaa gaattagaaa ttacagcaat tactgataaa
     4801 ggggaaataa tggggattag gcataagaaa tatttaatag aaggagtaca gtttcatcca
     4861 gaagctattt tgtcagagta tggtcatgaa atgcttgaaa attttataac agaagctaga
     4921 gaaagggtac atatgtaata tgataagaga gataaatact aaattaaatt cttttgagat
     4981 atttactatt tttagaaatg aacatgacag ttttatatta gacagtgcta tggataaaga
     5041 aaaattggga agatattctt ttataagtag ccaaccattt aaggtattaa agtataagga
     5101 tactgatgaa aatcctttag aagttttaaa agaagaacta cataagtata gggttgtaaa
     5161 tgatactaat cttccatttg taggaggtgc tgtgggatat ttatcttatg atttaggtaa
     5221 ttacatagaa aatttaccta gaactgctgt agatgatatt gagatgcctg atatgtattt
     5281 tggattttat aatcatgtaa tagttataga ccatcttgtg caaaaaactt atatagctac
     5341 tccaaacata gatatcgaat tagaagaaaa gataatagat gatatagaac aaagaatatt
     5401 aaaagaagag aaaaaaggta tagatagcat atgttatgaa gaaaaagaag taacacctat
     5461 aagacttaaa tctaatttta caaaggaaga atttaaaaat gcagttcaaa gtgttagaga
     5521 gtacataaga caaggagata tatatcaagc taatttaaca caaaggttca gtggagaaac
     5581 agaacttaca agctttgaat tgtataggga cttaagaaga tttagcccag caccatttgg
     5641 ggcattctta aactttgaag atacacatat attatcaaat tctccagaaa gatttataag
     5701 atgtgttaat aagagaatag aaactagacc aataaaaggt actcgtccaa gagggaaaga
     5761 taaagaagaa gatttaagac ttcaacaaga acttagaaat agtgaaaaag atagagctga
     5821 actacttatg atagtagatt tagaaagaaa tgatattggc agaatatcta aaactggaag
     5881 tgttaaagtt ccagagctat ttgtaattga accatatgca aatgtaaatc acttagtttc
     5941 cacagttgta ggtgagttaa aagatgataa agatgctact gatgtaataa aagcaacttt
     6001 tccagggggg tctataacag gagcacctaa gattagagca atggaaatta tagacgaatt
     6061 agagccaacg caaagaaatg tatatactgg ttcaataggt tatataggtt ttaatggtga
     6121 tatggatttt aatatagcaa ttagaacaat aatcaaaaat gacaaaaaag tctattttca
     6181 agttggagga ggaatgactt gggattctga cccagatgaa gaatatcaag aaacacttga
     6241 taaagctaaa tccataatga aggctttgag gggatattat gaagaataat gtaggttttg
     6301 atagtagttt aagtaaattt ggaattggtt tgtttgaaac tattagagtt aaaaatggaa
     6361 tagcagtaga tttagatatc catatagaaa gaatgctaag ttcaataaat tgtttggatt
     6421 tagatataaa ctataaaaaa gatttcttga taaatgaaat tgttacatat ataaaaaaag
     6481 aaaatgtcat taataaagca cttagaatta ctgtttttga tgaaggttat aatatatcta
     6541 ttagggatat tccatataat caagagacgt atgagaaggg gtttaaatta gctatatcgc
     6601 caatagttag ggggaatagt ttaattcata gacataagac tactaattat tttgaaaaca
     6661 tatataccaa aaatatcgca aataagactg gttataatga tggtatattt ttaaattcag
     6721 aaggtgtaat attagaatgt tctatgagca atattttctt tataaaagga gaaagagtgt
     6781 acactcctag tgatagatta ccgatattaa atggaataat aaaaaaaagg attatagaga
     6841 tttgtgacga attacatata gagttgatag aaaatgaaat taatatatca gaaattagta
     6901 gttttgattt tgtttttgtc acaaatagtc ttatgggcgc tataaaggtt acagaaattg
     6961 acaaaataaa atttaataaa gaaaatgttg tttttgataa aatagtgaag ttattagaat
     7021 aaattaaaat attaacaatt tgttggagac aaaagatgat aggttgtatt tatgaagtat
     7081 acaatgaaga gatgactaaa ataatagaaa accaaaacac tttggctgta tatttagtag
     7141 gttctagtaa agacgtagat tttactcttt atgatgtaga gataaacgat attgatgtgt
     7201 tcgtttttgt aaaagaaggg gaaaaacaag agagaatact gtttaaaaaa aagggaatag
     7261 aatttgatgt aaattacttt tcaaaagatg gtgtaaaaaa attattatca aatagggaat
     7321 atttctttgt gaaagaaatg aaagatgcta aagtactgtt tgatagggag aatatatcaa
     7381 atattataaa agatataagt agagatattt acttagaagg acccagtaaa atgtcattag
     7441 aggaaaagtc ttttataaag caagatattg gagcaaaaat atcgaattta aaaaataaag
     7501 aaaaatttga tgtttttgaa tatcattttt tgacgaattt atatttaaaa gatataataa
     7561 ttgggtactt taatataaat gataaatggg taccaaaaga caaaaaattg ctaaaagttt
     7621 taaaaaaaga aaataatgag ctttttgaac tagcgtcaaa agtaagtgaa aattatgact
     7681 atcaaagact tttagatgtt tataattata tttttaaaga aattgagaca aaagaggtta
     7741 taaaactaat cttctagtca caaatcaatg gggggagttt aaatgaataa agttgataaa
     7801 gaaaagatac aacatgcagt aagagaaata ctagaggcaa ttggagaaga cccagataga
     7861 gaaggtctaa tcgagactcc aaatagagtt gctaggatgt atgaagagat attctctggt
     7921 cttagtgaag aaccaagaga ccatttaaaa gttctttttg cagatgaaaa acatgaagaa
     7981 ttagtattgg taaaagatat tccattttat tcatgttgtg agcatcatct agttcctttc
     8041 tttggaaaag ctcatattgc ttatttacca aaaggaggaa gacttacagg gctttctaaa
     8101 cttgctagag taatagatac attggcaaaa agaccacaat tacaagaaag aatcactaaa
     8161 aatgctgcag atataataat ggaagaatta caaccttatg gtgtcttagt agtagtagaa
     8221 gctgaacata tgtgtatgac aatgagaggt gtaaaaaaac caggttcaaa aactgttaca
     8281 tcagcagtga gaggaatatt tgaaaaggac attgcgtcta gagccgaggc tatgagtttg
     8341 attactatga agtagcaatt tattatagat ataatcattt attgagatat aaaatttaat
     8401 ttaagggagt gtcattaatt gtgtttgatt atggtaaaag aacttatata atgggaatat
     8461 taaatgtaac tccagatagt ttttctgatg gaggagattt taataacttg gacatagcaa
     8521 tacaacatgc aaaagatatg gttgaccaag gtgcagatat aatagactta ggaggagagt
     8581 ctacacgccc aggtcatagc tatgtagact cagacgaaga gctaagaaga gtaatccctg
     8641 taataaaaaa acttaaacag gaattggaga taccaatatc aatagacact tataaggcag
     8701 atgttgcaga agaagcatta aaattaggtg tcactatggt aaatgatgtt tggggtctta
     8761 gaaaagataa aaatatggct tctgtaatag gaaaatatga tgcagaagtg tgtataatgc
     8821 ataatcaaga tgggacaaac tatgacaaag atataatgga gtcaataaaa gactttttta
     8881 aggtaagtat agaaatggca atgagttgtg gtgtaaaaaa agaaaaaata gtacttgacc
     8941 caggcgtagg atttggcaag gattttgaac aaaatattga agtattgaga agattaaatg
     9001 aattaaaaga tttagggtat cctattttgc ttgggacatc cagaaaatca gtaataggaa
     9061 aagtacttcc agttgagcct aaaaaaagat tagaaggtac aatagctact actgttcttg
     9121 gtataagaga tggcgtagat atagttagag tacatgatgt ttatgaaaat ttaatggcag
     9181 ctagaatgac tgatgcaata tatagaaagt aggtataata taatggataa aatattactt
     9241 agtaatctag gattctatgg atatcatggt gttcttaaag aagaaaattt tctaggtcag
     9301 aaattctttg tcgacatgga actttatatt gattcaagag aagcaggtct tagtgacgat
     9361 ataaataaat ctgtaagcta tgccgaggtt tacaatgttg ttaaagatat tactgaaaat
     9421 aaacaattta atcttttgga ggctttagcg gaaaatattg ctgaagaagt cttgaataaa
     9481 tttatactta taaatggagt tatggtcaga gttagaaagc ctgaggctcc tgtaaatggt
     9541 atttatgact attttggtgt tgagataagg agaacaagag atgaataagg cttatttggg
     9601 aataggtact aacatgggtg atagatttga taatttatca agagcatgtg aacttttgaa
     9661 aaatagtgat tctatatata aagtaaagga atcaagttta tatgaaacaa aaccatgggg
     9721 atatacagaa caagcagatt ttctaaatat gtgtgtagaa atagaaacag aatttgaacc
     9781 ttatgagttg ttagaatatt gtcaagaaat agagagagaa ctacacagag agagaatagt
     9841 tcattgggga ccaagaacta tagatgtaga catactattt tttaatgatg tagcttccac
     9901 tgatgaaaga ctaacaatac ctcatccgag gattcaagat agagctttcg ttttgatacc
     9961 acttatggat ttaaatgaag aacttataat aaatgaaaaa acagtaaaag aacatttaaa
    10021 cctcctatct gctgaagaaa gagaagaagt aaaggagctt gtaggttatg aaagaaaacc
    10081 tatttaataa taattcaaag atagagataa atacaaataa caaaaagata actgtaaaaa
    10141 aagataaact tataatagct ggaccatgtg caatagagag ttatgagcaa cttttggaga
    10201 ctgctaaatt tgtaaaaagt cagggagcaa atattttaag aggaggagcg tacaagccaa
    10261 gaacatcgcc aaattcattt caaggattaa aaaaagaagg tcttaaaata ttaaaagcag
    10321 ttaaagacga agttggtatg gctgttatta cagaacttat ggatgtaaga gatatcgacg
    10381 aactgtatag tataagtgat ataattcaaa ttggttcaag aaatatgcaa aactttactc
    10441 ttttaagtga agtgggaaaa caaaataaac ctgttatgtt aaaaagagga atagcatcca
    10501 caattactga atggataggt gcttctgaat atatagcaat agaaggaaat agtaacatta
    10561 taatgtgtga gagaggtata agaacgtaca atgattatac tagaaataca ttagatttag
    10621 ctgcggtacc cattattcaa aaggaaacag gtcttcctgt tgtggtagac ccaagtcatg
    10681 ccactggagt tagatatttg gtaaaaccta tgtcactggc atcttttgca tgtggagcag
    10741 atggaataat ggtagaagta catcctgacc cagaaaatgc tttgtcagat ggtgctcaat
    10801 ctctttgttt taatgaattt gaagatttaa tgaaatcaat taataattat taaaagaggc
    10861 aagagcctct tttttattcg taatcattgc taaggtaaat ttataagtta taatttgata
    10921 gttagattat ttaatataaa taaagattta atattgatag aattaatata aaaaataaaa
    10981 attttttgat taataagaag gatatcaata aaaaaagtcg aattgtgtat attgagtgat
    11041 tgacaaattt agaatatagt gtagaataga aatgttaaca caaaaaatta caccagaagg
    11101 tgataatatg aatgatataa aagacattat tgaagaaata aaagctagat gcgatatagc
    11161 aagtataata tctgaatata tgaatataaa acaatctgga gctaattaca aagggttatg
    11221 tccatttcat ggtgaaaaga caccttcttt ttatataaat acatcaaaac agatttataa
    11281 gtgttttggt tgtggtgaag gtggagacgt aataaatttt gtcatgaaaa tggaaaattt
    11341 ggattttatg gatgctgtaa aaattttagc aaataaatgc ggtatagaaa ttaacactaa
    11401 catgaatgaa gaaactagga ttaaaataga aaaatctaag aaatttcaag atatccatac
    11461 ggaagcagct agattttatc tttcaaactt attgggaagc aaaaatcttg ggtatgaata
    11521 tttaagaata agaggactag atgataaaat tattaagaaa tttggtttag gattttcttt
    11581 ggattcatgg aattcactta tgaatactct aataagtaaa ggatacaaaa aacaagactt
    11641 attggattgc ggtctaattg caaaaaatag agatggaact aattgctatg ataaatttag
    11701 aaatagagtt atgtttccta tatttgacta tagaggaaac ataataggat ttgggggaag
    11761 agtattagat gattctttgc caaaatactt aaattctcca gacacattaa tttttaataa
    11821 aaaacagaac ttatacggat taaattttgc tagaaaaaat ttagaaagta aaactatagt
    11881 cttagttgaa ggatatatgg atttaatttc gctttatcag tatggtataa aaaatgtagt
    11941 agcaaccctt ggaacagcct tgactgaaca acaaggcatt ttaataaaga gatatgcgga
    12001 cactgctatt atatcttatg attctgatga agcaggaata aaagcgactt taagagctat
    12061 agatatactt actaaattag gtatcaatgt aaaagtgtta gacttaaaag atgctaaaga
    12121 cccagatgaa tttgtaagaa aatacggact tagtgattat aaaaaagcta tggatgtttc
    12181 tactcactac ataaaatata aaatagatca tcttaaaaaa gaatttaaca ttcaaaaaga
    12241 tgaagaacga gtgaagttcg caaaagaagc ttctaaaatt ataaaacaat tgacaagtcc
    12301 tgttgaaatt gatttttata caaagtattt aagcaatcaa atagatatta atgtcgagtc
    12361 tataaagaga gaagtttatg gcaaaaatta caataagcca tataataaca aaaatcaaaa
    12421 gaaaatagaa gaaaaagtaa ttgaaaaagt cgaagtcaga caagacggaa aacaattagt
    12481 tgaagaaact ctaataaaaa taatgttaga agacaaaaaa attagagaaa tagcgttact
    12541 taaagtagaa gaaagtgatt ttttattgaa agaaagtaaa gaaattttaa attacatgat
    12601 taaaaatcaa gaattggaca aaataactat tgacaaattg aaaagtttaa acatatccga
    12661 agaatactta aaagaattaa attcaatttc tttgaatagt ataaacttag agaacacaaa
    12721 agaaatagaa ggaataataa caaatatcag aaaaaactca ttagaagagc aaattaatag
    12781 cttgttgaga gaacaacaag aattagaaaa taataatgat atgaaagagg tagatggtag
    12841 agttatggaa attgctttaa aaatagttga gataaataaa attttaaaga gcttgtagag
    12901 aaaggaggtt tgagtgtgga aaataaatcg aataaaaaag agttaaaaaa ggttactgct
    12961 aagacattaa tagaaaaagg aaaaaagcaa ggttctctga cacttgcaga gataatggaa
    13021 gctttttcgg agactgaact tgataaggat caagtagaaa atctttatga gactttagga
    13081 aatttgggaa tagaaataac agaaacaaaa aattataaag ctgatataga tttttcggtt
    13141 gctgatgacg atttaagtat aggccactta gatgaagatg cagaggcaat ttcacatgat
    13201 gactcttctg caatagaaat agaaactgtg gatttatctt taccaaaagg gataagtata
    13261 gatgaccctg taagaatgta cttaaaagaa ataggaaaaa ttcctctact taaaccacat
    13321 gaagaagtag aatttgctag aaggatgcac gaaggtgatg agatagcaaa acaaaggtta
    13381 gttgaagcaa acttaagact agttgtaagt atagcaaaaa gatatgtagg aagaggtatg
    13441 cttttcttgg atttaataca agagggaaat ttaggtctta taaaagcagt cgaaaaattt
    13501 gactatacaa aaggatataa gtttagtact tatgcaacat ggtggataag acaagctata
    13561 acacgtgcta ttgcagacca agctagaact ataagaatac cagttcatat ggtagagact
    13621 ataaataagc taataagagt atcaagacaa ctacttcaag aacttggaag agatccaaaa
    13681 ccagaagaaa ttgcaaaaga aatggaaatg acagaagata aagtaagaga aataatgaaa
    13741 atagctcaag accctgtgtc tttagaaaca ccaataggag aagaagaaga tagccattta
    13801 ggtgatttta ttccagatga tgatgctcca gccccagcag aggcagcagc atattcttta
    13861 ttaaaagaac aaatagaaga tgtacttggt tcgttaaatg atagagaaca aaaagtatta
    13921 aagcttagat ttggtcttga ggatggtaga gccagaactc ttgaggaagt tggaaaagag
    13981 tttgatgtaa ctagagagag aataagacag atagaagcaa aagcactaag aaaactaaga
    14041 catccaagta gatcaaaaaa acttagagat tatttagact aaaaagatac atcattgatg
    14101 tttttgtgat aaatgccatg agataagtga tatctcatgg ctattttgta gtaaagattt
    14161 aattttattt ttgtagttat ttcagtctaa tatctataac ttggattaaa tcctataaat
    14221 aaagggaatt gaaaggagaa ttatatttga aattaacaga tagattattg aaaatagcat
    14281 cattagtgtc agatggtaaa aaaatagctg atatagggac ggaccacgga tacataccag
    14341 tatatttgtt aaaagaggga aaagtaccat ttgcagtgct agcagatgtt aataaaggac
    14401 ctcttgacaa tgcacataaa gaagtcatac agaacaatct tttagataaa gttgatttac
    14461 gattgggttc tggtatagag atacttgaaa taggggaagt agaagaagtg ataattgctg
    14521 gtatgggtgg aatcttgata agtgagcttc tcgaggctaa aaaagaagta gcacataatg
    14581 tagaaaaatt aatattacag ccaatgcaag cacaagaaga acttagatat taccttttaa
    14641 acaacggata tgaaatttta gaagaagttt tagtgagaga agattttaga atatatgaga
    14701 taattgttgc caaatataca ggtaaaaata ctataataga agatgagata tattatgagg
    14761 ttggaataaa gcttttagaa aacaaagatt ctctttttaa tgattttata gaaaaaaaga
    14821 taaagacata tagttctata gttaataaat tagaagggaa aaatggggaa gctatagata
    14881 agaagagaaa ggaaagcgaa gttgcaataa aaaaacttga aaatttaata aaataaatag
    14941 gaggtatata tgctattaaa gagtctaact agaaaaattg aaaaaaagta tcctctaaac
    15001 ttagcagagg attgggataa tgtaggtctt atagttggag attttgatat ggatgtaaaa
    15061 aaggtattgg tatctttaga agctaatgaa gatgttattg atgaagctat atccaaaaat
    15121 atagatttaa ttgtgacaca tcatcctttt atatttggga aaattaataa aattaatagt
    15181 ggagatttaa aaggcagact tatccaaaaa cttattaaaa atgatatatc actttattct
    15241 atgcatacga attttgatat agcatttgat ggtctaaatg actattttat ggaaattatg
    15301 gaatttggta attcaaaggt tttagatata actaaaagcg aaaatttgta taaattagca
    15361 gtatacgttc cacataacta ttcagatgaa cttagaaagg ttttaagtaa ttcaggagca
    15421 ggtcatatag ggaattatag tgattgtacg ttttcaatag aaggtgaagg acaatttaag
    15481 ccattagaag gctctaatcc gtttcttgga agtgtaaatg atatagaaac tgtaaatgaa
    15541 gttaaaattg aaactgtagt accacagaag cttttaggtg gggtaataag ttctatgtta
    15601 gatgcacatc cttatgaaga agttgcatat gatttgtaca agttagaaaa taaaggtgag
    15661 atatttggac taggtagaat aagtaaactt gataaaagta tgacattaga aagtctttca
    15721 aggaaaataa aagagaaatt aaatatgaaa cacataagag tagttggaaa ccttagtaca
    15781 gacataacga aagtagccgt tgtaacagga gctggttcag aatttgtaaa aaaagctaag
    15841 agacaaggtg cagaagtact aattacgggt gatgtaaagt atcatgaggc acaagatgca
    15901 cttgatattg gaatgtgtat agtggattgt ggtcattttg atacagaaga tatattcaaa
    15961 aatgttatga agagattttt agatgaattt agtgaaattg aagtaataaa gagtaatgta
    16021 tatttgaatc catttagtac tatttagaag tgtttgatta aaaaatacta tataaaaaat
    16081 aaatttgagt ttaattaggg ggataatgat gaaagtagca gtaatttttc atagtgtttg
    16141 tgggagtaca tatttattag ctagagaata taaagaagtg cttgaagaaa tgaatatcga
    16201 ggtagatata tttagagtaa gtgacgaagt tgcaaaaaca cttcctcaat attatttgat
    16261 aaattacaaa gaatacaaag acgaatttga aagtataaat gtaattaaat ctggaaaaga
    16321 aatattagat tatgatgcta tatttatggg ttcaccaaca tattatggaa atgtatctgg
    16381 accaatgaag atgtttatgg atagtttttc tgatatttgg gtaggtgctc ctttgagtgg
    16441 taaaatattt ggttgttttg ctacagcagg ttcacaacat ggaggaggag aattagcttt
    16501 gcaagctatg aatatatttg ctcaacatat gggaatgact cttttgtcag ttccatgttc
    16561 agtaagagga ggttatccag cgtatggaat acttcatata gcaggagata attctgatat
    16621 aagaccaaat gatgatgcca agataggtat aagagattac ctaaaaagac ttaacatata
    16681 agaatatcat ataaaaatac ctaaaagtag ctgtatatac ttttaggtat ttttacttaa
    16741 ttataaacgt gaagctttac cagaaatatg gtcaatttct attttaacaa cagttgttaa
    16801 gttcatatcc ttctctatgt acttcatgcc ttcttcaaag aatcctttag aatatttttt
    16861 tattatttct acaagagcat ttttcttttc ttcattttct acagtgcaag cttttccaaa
    16921 aactatagca ctagaataag tagtactaaa tttatctgga ataacacttt cattagcaac
    16981 tactaaaaaa gaaactttgt tgtttttcga tatattatct aacttatgtc catttcttgc
    17041 acaatgaaag taaatagaat cattaaagta tacataattt acagcaactc cataaggata
    17101 accattttca gatatagtgg aaaaagtacc aaattcacca ttttttaata cttcaacagt
    17161 atcttctttt gtcatttctc tttttttcaa tctcatttct ttaaacataa tatcatcctt
    17221 tctaattttg atgcttaaat taaattttag cacatttata aagattatgc tatgtttttt
    17281 acaaatttta agaaaaaaat atagtttaag ataaaaggta gtgaaagaga agctatttgg
    17341 aggtttattt atgatattat tgttagagta gtacaaacaa tactaataaa tttaatagct
    17401 ggtttcttgt tatttaaggt agtaagcatg tctataagaa ggggaagtta tcgagggtag
    17461 aagttgttat ttataacgat attataaaga aaatattatt atataaaata ataaatagta
    17521 aagaggtatc aagttgaata taatacttaa tacctcttca aaattaaatt ataaaagtgt
    17581 tgtgggaatt gtcctataca tggtaaggag ataataaaga atgtatcttt aaagtctcaa
    17641 ctaaacagca aaatttatcc aacaaacata caataaatgc ttctttgtat ctaggtggct
    17701 taacagtaag tggccacatg tgttttaaaa ttatgtccgt ttctttttca tttatttgaa
    17761 aaaataaagt tgcatttttt aatgcttctc tagggtgagt aaatccatgt aatccttttc
    17821 tatcaccttt gtaatgccaa tcataaagaa aaaagtcgtg tagtaaagct cctctaataa
    17881 cactttgaat atcaacacgt aagtgcaatt ttttacataa tagataacta taatatgcta
    17941 ctgacaaact atgttcaaga catgaaacat tgccatgatg aggaaatgtt ctcataagtt
    18001 gaactttttc agaagaaagt atctcagaag cacattcatt aaaatatttg atttcagctt
    18061 ttgacaacat tatgaaaatc tccattctta gtaatatata aatactatac ttcaacgata
    18121 atatgaaata tttcttatcc tacaaaatat aatacccaat aatacaagta agataccttt
    18181 tatggtattt taaagtaaga gagattttat taggctaaac aatgaaaaaa ttaaaagtaa
    18241 agctatagca atatatagta tttttccaag aggattgcca aaatttatag tccatccaat
    18301 accaaacctt ttattaacca ttaatgatgg gtcatttgga ttattataca tgatgcctgc
    18361 tatccaatac ttctcatctt cttctggaga ataagatgag ttgaatttta aatttgggga
    18421 attaatatat gttgctatta aataaattat aactaaaagc gttgtaatat tcatagcagt
    18481 tgatagtttt gtttttattg ataaaagagt agtaaaaaac tgtgttgtca ttataatcat
    18541 caatattaaa aacaaataac caattttatt taaatattta atgttttcaa ctctactttc
    18601 ttcaattcta tttgtatcta tcttaatcct tgactttatg gatccaatgg aagttatata
    18661 taataaaacc attaaacaaa agtcagtacc gattaattta aatacattaa gaaatgattt
    18721 atctataaaa acatcaggag acccatttat gttccaatga gtggcaattg agtctggcaa
    18781 tttattataa ttaaatgcta taaaaatact catccccaaa gtaaatagta tcggtattaa
    18841 atagattatt ttaaattttt ttattatttt atttttctta tttataaaat ccatatctac
    18901 tattaatttt gaatctacat ctatatgacc taatttagaa taaatttctg atttaagctt
    18961 cttagct
//
LOCUS       DACTGW010000061        18920 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.061, whole genome shotgun sequence.
ACCESSION   DACTGW010000061 DACTGW010000000
VERSION     DACTGW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18920
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.061"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(114..2390)
                     /gene="slpA"
                     /locus_tag="I9573_001648"
     CDS             complement(114..2390)
                     /gene="slpA"
                     /locus_tag="I9573_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HAT4796274.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFAAEDMSKVETGDQGYTVVQSK
                     YKKAVEQLQKGLLDGSITEIKIFFEGTLASTIKVGAELSAEDASKLLFTQVDNKLDNL
                     GDGDYVDFLISSPAEGDKVTTSKLVALKNLTGGTSAIKVATSSIIGEVENAGTLGAKN
                     TAPSSAAVMSMSDVFDTAFTDSTETAVKLTIKDAMKTKKFGLVDGTTYSTGLQFADGK
                     TEKIVKLGDSDTINLAKELIITPASANDQAATIEFAKPTTQSGSPVITKLRILNAKEE
                     TIDIDASSSKTAQDLAKKYVFNKTDLNTLYRVLNGDEADTNRLVEEVSGKYQVVLYPE
                     GKRVTTKSAAKASIADENSPVKLTLKSDKKKDLKDYVDDLRTYNNGYSNAIEVAGEDR
                     IETAIALSQKYYNSDDENAIFRDSVDNVVLVGGNAIVDGLVASPLASEKKAPLLLTSK
                     DKLDSSVKAEIKRVMNIKSTTGINTSKKVYLAGGVNSISKEVENELKDMGLKVTRLAG
                     DDRYETSLKIADEVGLDNDKAFVVGGTGLADAMSIAPVASQLRNANGKMDLADGDATP
                     IVVVDGKAKTINDDVKDFLDDSQVDIIGGENSVSKDVENAIDDATGKSPDRYSGDDRQ
                     ATNAKVIKESSYYQDNLNNDKKVVNFFVAKDGSTKEDQLVDALAAAPVAANFGVTLNS
                     DGKPVDKDGKVLTGSDNDKNKLVSPAPIVLATDSLSSDQSVSISKVLDKDNGENLVQV
                     GKGIATSVINKLKDLLSM"
     gene            complement(2616..4205)
                     /locus_tag="I9573_001649"
     CDS             complement(2616..4205)
                     /locus_tag="I9573_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HAT4796275.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGSPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNIDTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYSLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVINGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            complement(4424..6025)
                     /locus_tag="I9573_001650"
     CDS             complement(4424..6025)
                     /locus_tag="I9573_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HAT4796276.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADRQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSYDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKNLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            complement(6392..8272)
                     /locus_tag="I9573_001651"
     CDS             complement(6392..8272)
                     /locus_tag="I9573_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HAT4796277.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSVTCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKITKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSNSTSNVTVKKLKGADRFETA
                     TKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAVQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVVDKIKSLI
                     EK"
     gene            complement(8482..14445)
                     /locus_tag="I9573_001652"
     CDS             complement(8482..14445)
                     /locus_tag="I9573_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HAT4796278.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGN
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNIGTDNIEYTIK
                     KLSASRSEIKNKDENVSYYNIVFNNLPVGKYNIEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDEDGNVQQ
                     IPYENKEYMKNLKDKEQQEEVNNEQEEVNQEEVNNKDENKNQTDNNSNNKDENNDKQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEGDNISKDDNSTTSTYSRAALE
                     TYTDVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSLKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESIDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEIRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQ
                     EINKSGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLKTGKNVIQLSKLHSLDIECGGSLYIRYPEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNIGNSY
                     EYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKAKFTDSISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDVSTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAETRKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDDKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDPSTSEDETGKIPAGS
                     IIITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAIDVSNKKVITVSNKVKGNIVRDK"
     regulatory      complement(14569..14654)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(14826..16229)
                     /locus_tag="I9573_001653"
     CDS             complement(14826..16229)
                     /locus_tag="I9573_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HAT4796279.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILIDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALVASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     DNGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKISDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(16691..18586)
                     /locus_tag="I9573_001654"
     CDS             complement(16691..18586)
                     /locus_tag="I9573_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HAT4796280.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKSETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         5322 a         3533 c         2073 g         7992 t
ORIGIN      
        1 taagtatttc tcacataaaa aagagactaa atttagtctc tttttttgta ttcgaaaaca
       61 taatataaaa agacttctca tataagaagc ctttttatat tattatattt atattacata
      121 cttaataaat cttttaattt atttataact gaagtagcta tacctttacc aacttgaact
      181 aagttttctc cattatcttt atcaagaact ttacttatag atacactttg atctgaagat
      241 aaagaatcag tagctaatac tataggtgct ggagatacta atttattttt atcattatca
      301 gaaccagtta atactttacc atctttatct actggcttac catcagaatt aagagttaca
      361 ccaaagtttg ctgcaactgg agctgctgct aaagcatcaa ctaattgatc ttctttagta
      421 gaaccatctt tagctacaaa gaaattaact acttttttat cattatttaa gttatcttga
      481 taataagaag attcttttat aacttttgca ttagttgctt gtctatcatc tccactatat
      541 ctatctggag atttacctgt agcatcatct attgcatttt caacatcttt agatacactg
      601 ttttctccac ctattatatc aacttgtgaa tcatctaaga aatcttttac atcatcattt
      661 atagttttag cttttccatc tacaactact attggtgtag catcaccatc agctaaatcc
      721 attttaccat tagcatttct taattgagat gcaactggag ctatactcat ggcatctgct
      781 aatcctgttc ctccaactac aaatgcttta tcattatcaa gacctacttc atcagctatt
      841 tttagagaag tttcatatct atcatctcct gctaatcttg taactttaag tcccatatct
      901 tttaattcat tttctacttc tttagatata gaattaactc caccagctaa ataaactttc
      961 tttgaagtat ttatacctgt tgtactcttt atattcataa ctctctttat ttcagctttt
     1021 acgcttgaat ctaatttatc ttttgaagtt aataataaag gagctttctt ttcagaagct
     1081 aaaggagaag ctacaagtcc atcaactatt gcatttcctc caaccaatac tacattatca
     1141 actgaatctc taaatatagc attttcatca tcagagttat aatatttttg acttaatgct
     1201 attgcagttt ctattctatc ttctcctgct acttctatag catttgaata tccattatta
     1261 tatgttctta aatcatccac ataatctttt aagtctttct tcttatctga cttaagagtt
     1321 aatttaactg gtgaattttc atcagcaatt gaagcctttg cagcactctt agttgtaact
     1381 ctttttcctt ctggataaag aaccacttga tattttccac taacttcttc tactaatcta
     1441 ttagtatctg cttcatcccc atttaatact ctgtaaagag tatttaaatc tgttttatta
     1501 aatacatatt ttttagctaa atcttgtgct gttttactag aactagcatc aatatctatt
     1561 gtctcttctt ttgcattcaa tattctaagt ttagttatta ctgggcttcc agattgtgtt
     1621 gttggtttag caaactcaat agtcgcagct tgatcatttg cacttgcagg tgttattatt
     1681 aattctttgg ctaaatttat agtatcacta tctcctaatt taacaatttt ttctgttttt
     1741 ccatctgcaa attgaagacc tgttgaataa gtagttccat caactaaacc aaacttttta
     1801 gttttcatag catcttttat agtaagtttc acagcagttt cagttgaatc tgtaaaagct
     1861 gtatcaaata catctgacat agacataact gcagcactac ttggagctgt attttttgct
     1921 cccagagtac cagcattttc gacttcacca ataatactac ttgtagctac ttttattgca
     1981 ctagttccac ctgttaaatt ttttaatgca acaagtttac ttgtagttac tttatctccc
     2041 tctgctggag agcttattaa gaaatctaca taatccccat cacctaaatt gtctaattta
     2101 ttatctactt gtgtaaacaa taatttactt gcatcttctg cactaagctc agctcctact
     2161 tttatagtag atgctaaagt tccctcaaag aaaattttaa tctctgttat acttccatct
     2221 aataaccctt tttgtaattg ttcaactgct ttcttatact tgctctgtac tactgtatat
     2281 ccttgatcac cagtctcaac tttcgacata tcttctgctg caaacacagg tgctgcagaa
     2341 gctaatactg ttaatcctga catagctatt gctatattct tcttattcat ttcttaaatt
     2401 cctcccaaca ttatattaaa attacatctc ttcattatgt acatagaata atcatccata
     2461 tattcctaat atttctcaca tctctataat aatccatttt tattcttttt gcaagtcatt
     2521 tttttgaaat tattattttt cccaagcata tgagttttta tacccttaaa ctccaatatt
     2581 taataactta aaaaatttga ttttacttat ttaaattact tatttaaaaa ttcttgtgta
     2641 gcatacactt ctccattagg caatatatat aatccaaacc cagtatatac aaaatcacta
     2701 tttaacatat tttcattata tttatctgac tttttccaaa ctgtaaatat tacatttgct
     2761 acttctctag catcctttgt tgtataagta ctttttgtaa caagtggtac aaaagctata
     2821 ttttcttcac ttctcaagcc atatccagta aatacttcaa cagatttctt accatttatt
     2881 acatgtgaaa actgaccttt atctgccatg tacttagacc aggaattaga aagtccttct
     2941 aagtttttag aaacttgtaa tggctcttta cctttttctt ttctatatga atttaccaaa
     3001 ctatacattt cactagaaac cattctttta aactcactac tattcatatc attagtctta
     3061 tattcaagta catttacttc tacacttatt gatgtagtct ttccaccaat atcagtagct
     3121 tttatagtaa ctttgtattt acccacctta tcagtatcaa tatttcctat aagctctggt
     3181 gtcaaatcgt taccattgct atcctttgct acgatattca gtttacttat atcaaactta
     3241 tcaccttgat aaatatttaa atttccaaca gttattgttg gtgcacttgc atccagtgat
     3301 gatgcactta aacattgttc tagtgtcttt tcatctattc ctcctagtgc tgtaatctta
     3361 tcagcacttc taagtacaga cttatcacta tttccgtcaa ctaatacaat aggtgcattt
     3421 tttgctatag aagaaccagc aactgcatct actaattgat aacctttaga aagataaaat
     3481 tcttttgccg aactataaaa atgttttata acttttttat tagtctcaaa tctatcttct
     3541 ccagctaatc taactgcatt agttttttta actaaatcat tacttattat ctcctctgaa
     3601 ccaagagcat aacattgcac accttctttt ttctcaaatg gaacattttt accattagtt
     3661 aaaattatag gtgatccatc tcttgatgct actgatgacg cactcatagc atctgcttca
     3721 cctgtataac cattagttat aaaaacctta tctataggtt ttattgaagc tatttctttt
     3781 gctataagat aacttgtttc aattctatca ttaccaccta ttcttttaac ttctattcct
     3841 ttttgcttta attcattttc aactgatttt cctattgaat cttcagttcc tattatataa
     3901 accttcttta catcttttaa cattttttct gtgtctgatg gtatactgtt tctttgtgat
     3961 aataatatag gtgctttaac tgttcctgct aatccacttg cacttaatcc atcagctagt
     4021 gtattatctg tatttactaa tactacattt tcatatgttt gtttctgtgc aattttggca
     4081 gctgtctcat atctatcttt cccttgtatc ttttctatgc tcgaaagtgc attgatattt
     4141 ggtgctcctg ccattattaa tgatattgtc aatcctattg ataatactct cttatttacc
     4201 ttcatcgaac tcctcctact aattttttta tataattcag ctaatcataa attacatcct
     4261 cagtagtgat tccctcaaaa tctataaact actaaaacaa aaaacaccta aaataccata
     4321 gcctgtaaag caataacaaa acagattaca aaaagtactt tagatgtgat tttcatttta
     4381 taatatcagt aaattaacaa aaaaacaaga tttatggaat tttttacaaa ctaccttcat
     4441 tgccatttaa aaactcctga gtagcatgca cttgaccatc agataatata taaagcccaa
     4501 atccagtact atagaactca tcactaagca tgtttgcatt gtactctgga gacttcttcc
     4561 acacttcaaa tatagccttt gccaagtttt ttgcatcttg tgtcgtttga acgttcttag
     4621 aatctatata aatatatgct atattttcct cacttctcat tccaaactca gaaaatactt
     4681 gtggagcatt ttttccatct atataatgtg caaatacttt tttatccatc atatacttag
     4741 accatgcatt tgccatacct tctagcctac tagatactac caaaggctct ttgccttttt
     4801 cttttctata ggaattgatt aagttataca tttcagtaga taccattttt ttaaattctg
     4861 ggctgttgta atcataggat ttatcatcca acacttttat ttctactctt tttcctgtac
     4921 tttttcccca ttcgtctgta gcttttaatg tcaatatgta tgtacctact ctatttgtat
     4981 ctataaaccc atcaacttct attggtaata ccttcccagt gttatcttta gccactatgt
     5041 ttaacttgcc agtgtcaaac ttttcaccct tatatatctc tgtacttcca actgtaattg
     5101 ttggtggttg tccatttgac tttgaagcat ttatacactg ttgtattacc ttctcattta
     5161 tttcacctac agaagttata ttcttagctc cttctaatac agttttatcg cttccatcat
     5221 ttactagtac cattggagag tttttagtaa gtggtgcagc tgctattgca tctactaatt
     5281 ggtatccatc tgatacatag aatccatctg cactattaaa aaagtagtct attacatttt
     5341 tgttagtttc aaatcggtca gttccttcta ttcttactga gtttgtattt ttaactagag
     5401 ggttactcat tatctcctct gaacctatac aatatgattg aatatttttt acatcaaaag
     5461 gcacactctt tccatctgta agtatgattg gagctccatc tctagtagct actgatgata
     5521 cactcattgc gtctgcttct ccactatatg cgttagttaa tagtacttta tccacttttt
     5581 ttatagttgc tatttcttta gctattaaat agcttgtttt tagtctatct tctccaccaa
     5641 ttcttttgac ttctatattt ttagaatcta gttctttttc tactgattta cttatagtat
     5701 cttctgtacc tattatatat gcttttttga tattttttag acatctattt gtgtctgctg
     5761 gtatcttatt ttgttgtgta aatagtattg gagcttttgt agctcccgat aatccacttg
     5821 cacttaatcc atctgcaagt gatttttctg tatttactaa aattactgtt tcataagtct
     5881 gtctatcagc tatctttgct gctgtttcat atctatcttt accgtatatt ttttctacta
     5941 ctgatgatgc atttacactt ttgaagttta ctaagattaa tgatactgct agacctaatg
     6001 ataatatttt tttgttcatt ttcatatccc cctgatgatt tttatatttt atatttatat
     6061 tgtgattatt ttctatttat tttctcttta ttttccatat attatagagt atatatgata
     6121 tgaatatatc tcgtctatat attagtgttt tattttatta aatttaaaaa attattttta
     6181 acttcttttt aaacatattc aaaattgaat ttatatttat atatataaaa gatatctgct
     6241 ttatttaaaa ttaaacttaa tatagctcaa attattatac attttaacct caatattatt
     6301 aaattgttaa gataatgaaa ataaaataag aatgcatcta aatatataac tttatatcta
     6361 aatacattct tattttcgat ttacttaata tttatttttc aattaaactc ttaatcttat
     6421 ctactacaga tgaggctatt cctccaccta cttcaaacaa ctttgatgaa tttttacctt
     6481 ttaatgatga ttcttgtgat ttatctaacg tatttgtagc aagtataact ggtgaattgt
     6541 ttttagctgc taatggtcca actgctaaag catctataag tttatcttga tttgcaacta
     6601 caactccatc tagtttagat tgagagtaga atttatctat tactttggca ttcgtctctt
     6661 gtctagtttg acctgccacc ctattgtttg atatatcttt gtttgctatt tttccaactt
     6721 gttcaatagc cttgttagat attttttcta ttccaccaat aaagtatata tttgacacct
     6781 tatttgattt aataaattgt acagcttcat tatctacacc atctttttgt gtaagtacta
     6841 ttggagtttt ttctttaccg gccagtgatg ctattgataa tgaatctgct tctccatttc
     6901 ctgcccctat atacaatttg cttacattat tcgtcttatc tatttctttt gctatattaa
     6961 tagaagtttg atatcttgta tctccaccta ttctctgtac tgatattttt gaatttacag
     7021 ctttgatagc ttcaacaact gaatttggca ttgagttgtt tccacctatc acaattactt
     7081 ttgatggatt tagccttttc aattcttcta cagttttttg aggaagcttt tcatttgatg
     7141 ataaaagaat cggtgagttt tttacactag caagtggtgt agctgtaagc ccatccacca
     7201 tgcttttatc ttctccattt actattacaa ctgtatctga ttttgtccaa ccactttgag
     7261 atattttggt agctgtctca aatctatcag caccttttaa ttttttgact gtgacattag
     7321 atgtagaatt acttgaactt cctcctccag aacttccacc actagacgag ccgcctgaat
     7381 tacttccacc accattattt ccaccattgc cattatctgg tggtaattca tccggttttg
     7441 ataattcatt ttcaactatg tattctaaac tttttggaag tgtaacttca acatatttat
     7501 ttattgtagc ttttcttccc ttagttattt tttgtgctaa tccaagtttt aatattacat
     7561 ttgaaccact ttcttgtgtt tctttactaa caatagaata gtttatccta atattgttta
     7621 ataaatattc aagtgcttca cttttctcta ccttatactt ctcagggtca tttttactag
     7681 aaccagttcc accaattggt tttacttcac tctcattaat aagggtaaat attaatcctt
     7741 catcagagtt tatattttca taatccactg ttttgttgtt atatgttaca atttttaaag
     7801 ctggtttatc gcttgttcct tttcctaaaa ctttatagtc agcatttgag ttactgccag
     7861 atttttttac agatattctt cctatattca atctatttcc gtctagtaaa tcttcatttg
     7921 atgtaacata tatatctaat gtagttgtat tacttgattt gttgtattct actctacaag
     7981 tagcatttgc atttccactt tttatctgag attttatatt atcagaccaa gtaatctttt
     8041 ctttctgctc attatctact acttcaacct caccttgtac ttttatactg gcttgaaatg
     8101 aatatactat gttttctact gagtccacag atatatctat agtatttact ttctcatacg
     8161 tttttgttcc ttgtaaaact ggagctgcat atgcacatgt aacactacca gttacagcta
     8221 ctgccatagt tgatactgct atagttctta taaaagattt tcttttactc attaacaccc
     8281 gtccttatct atcattaact aagtggataa atttacttat ccacctagct tttatattta
     8341 atttataaat taccctaaac ttatagttac atttatatac tttgctttta atatctacta
     8401 ttttaaaact attctagtac ctaatcatat tagatataca atgttagtat tagtattttt
     8461 acgtttttag agttccacct actatttatc tctaacaata ttacccttta ctttattgct
     8521 tacagttata accttcttat tactgacatc aatagcattt cttgtagctt ttctagcttt
     8581 actattacta tcagtaagac ttattgtctt taactcatca taattcttag ctcctacagg
     8641 tacatacatt gtatcactta caaatctttg acctactggc gtatctcctt gaaggtcatt
     8701 gtatctatat agttcagcct taaccttatc acttaaagaa tcaaagtttt cttttggcat
     8761 atagtaaacc catgtacctt tactgatttc acctaattca cctttttctg gaacttctgc
     8821 aaataaaagt gcttctccag atatagtctt attgttttta tctataagta gtggtaaatt
     8881 aaatgctgga tttccactgt attcacctgt aataattatt gaacctgctg gaatcttacc
     8941 tgtttcatct tcacttgtgc tagggtcatc actttgatat acataatctt ctgctaactt
     9001 tccaattcca ttagtacgag tttggtctgc attattcatt gtgccaattt ctatattatc
     9061 acctggaggt cctattatat ctaattctgc aacttctata ttagacttat tgtttgagat
     9121 tagttttaca tatctagctt ggtatgtcca aagtttatca tctttattaa atgtaactct
     9181 agcttcatta tcttggtctt ttcctccacc aactaaatct tgtccatatt caaaagttcc
     9241 ttgacttaca tcttcccagt tctttttatc attacttact tgtatcttgt aattagttaa
     9301 aggattgtat gttgtgctag tattctttct aaagttaaat aatcctttta atgagaactt
     9361 cctagtttct gcctctggtt ttgtatattt tagtcctact ataggtaatg tttctttcat
     9421 gtctactatt atatatggat tttcttctac atttatatca gggtcttttt gagggtcttt
     9481 gttccactct gctttactta ttatctttcc tttatatgta gtacttacat cattatcttt
     9541 tatattatca atttcaggat tttgtaatgg tccatgtgag tcattttcag ttctaacatc
     9601 accgtcagaa acagtatttg ttatgaatga ccatgctgat ttatttattt tcccttcatc
     9661 tgataatttt acagtgccaa cttcaaattc ttctgttgga tttaggtgat aatcatatgc
     9721 aactacctta tatgtaaatg ctctgttatt aagaggagat atactatcag taaatttggc
     9781 cttactattt ccttttgcat ccacaaatcc aactgttctg tattttacct gtgttccatt
     9841 ttcaccatct aaataatttc catcacttct tattatttcg tatcctagta tcttatcttt
     9901 atccttatct acatctaggt ctatagatat tttattagat ttaactagag aaccactttg
     9961 tattccatca gaaaaactag cacttacttt agtatctttt gccatactag ctatattttc
    10021 atttaaaatc tttcttctag cttcgtcatt tacatattgt atctttcttg attctttttc
    10081 atagttctta ctatccatta tactgtttaa ttctggtgta atttctactc cccatgcctt
    10141 aaagtaatct cttaaatctt tttcaacaac acttgatgcc attgctacta gcatttggtc
    10201 ttttggtaaa ctattaattg ggtcactact tgaaagtgct ctgtattttc ttgaaatctt
    10261 tgcatagaat gtatcattac ttaaatcagc atctgagtta ttctttaaca ttgaacccgt
    10321 aaaatcatca tcataagcta gatgtaattg ccataacatt ccaagccttt caaatacatt
    10381 gcttggtata cctaagctat ttgaagttac atgctcatat atcttatcca ttgttgaacc
    10441 ttcaagtctt gaatgtgtct tatcatcaaa ggtctgagca agtaaagcaa gtacattgtt
    10501 actagtttcg ctatatgtca tctttccaat atctgtaaca tgtcctattt catgacctat
    10561 tccccatcca aataagtttc ctcctgtagc ttttccacta tcatctattt ggaaaggtac
    10621 tccattcata agccctggaa ctgaaccata ttctactcct acatgtaatc cagaagcata
    10681 cataaatgct ccaataaaca ttctttggta ttttatgttc atacgagatt ttggagccat
    10741 atcattttta gttaagttaa attcattatc atctacagtt ccattaccat ctaaatcttg
    10801 tgttttaaat actcctcttt ctgagtaaac taaatccatt atttgttccc atgcaagcat
    10861 actattatat aatctatcta cttgtgtatt taaatcagca tttccttttg atattccttg
    10921 atatacagct gttgcaggaa catttaatgt tactttatca ctttcaatat ctgtagtgtt
    10981 taacacacca gttttttctg aatattcata tgaattacca atatttgttc ctggataata
    11041 tggttctcct tttacagcat ctttatattt agctaattct tctatatatg cttttatctt
    11101 tttcttagat tcagaaactc ccgcaggact cttatcattt attgttccat ttagattaag
    11161 atgtggtatc ttagtagctc ctgctacacg tacttttata tctcctccac ttggagtatc
    11221 ttcagggtat cttatgtata aacttcctcc acattctata tctaaactat gaagtttgct
    11281 taattgaatt acattttttc cagttttaag atttatttct ccactcttat atgcaccaga
    11341 ttctccatag tactgtgtat atatcaactt aggagttttt ccacttttac ttccaacata
    11401 tactgttatt tcatctcctg ctctagcagc tactcctaat gcctgccagt cattttgcat
    11461 gcctaagtta ttggaattta cagtatactg tccgttttta tctttatatt ttcctgattt
    11521 atttatttct tgatttacag tagttacatc agtaagtgtg gctctatcat tatataagtc
    11581 ttgagctaat tttaattctc tatcaataac tgtcttatat ggatgatatt ctccactagc
    11641 tttatctgga gtatttaatc tatcttttaa ttcagttatt ttttcttgag ttacatcttc
    11701 ttttaattca acttgtaggt catccataaa tagatttctt acatcatctt ctatactgtc
    11761 atactcataa aacttaagtt ctgatatatt agacttatat gaataagatg aacaaacact
    11821 tacttgtatc ttagtagcag ttattggttc ttttaatcta accattacat acttattatt
    11881 atttacaggg tctgtctttg tttcaaaagg aacatccccc agattgtgta attgaccatt
    11941 ttcatcccaa tatcgtatct caaagtcatt tattgtctta tcagaaacat caaaacgagg
    12001 tgtagccata aaagtatcca tagtataaga tttatcaaaa gttattattg gtcctctatt
    12061 cgttccctca gctgcattat atgttgggaa tgtccaatgt gttgtgaagt ctccatctac
    12121 tacattgaat ttatcaaatg gatttttatc actatattca cttttgtcat atcctgctgg
    12181 ataaacaaca tctacaatgt gatttgttgg tattccaaat ccaccattct ccttaggagt
    12241 attaattaat ctatagttag gtgttgttgg tgcatctatg ctttcagttg tacctatata
    12301 tgttttagac attccactag ttcccaagtg gttagtagct gtcattctaa cttcatatgt
    12361 tgtgttatcc ttaagacctt ctatttttaa acttgtccca cgaataagct tatcattgta
    12421 ttttatccaa gacccattag agcctttttc tttatagtac aagtcaaagt ctttagcttg
    12481 tttatgattt ttccagctta catcaagaga tttaaattta cctttaacat ttataccttc
    12541 tggtggttct ggttttgtgt ctggaacaac ttgaaattct aatatgctat ctgggctaaa
    12601 ttgtataggt ttatagtttg agtctacatt ttctggtact ccatctaagt tttctggcgt
    12661 tgcatcttta taaccactag accaatctcc atttaaagat tgtatgctaa ccctatatag
    12721 tccatatgct tctatatctc taacttctat tgtattttta ctagtttgta attttttagt
    12781 agataatact tttcccttac tatcaatttt atctagtata agttcgtatg ctgatacatt
    12841 tacttcatta ttccaataaa aagatacttt ttcatttcca acagcagtgc tagtcgatat
    12901 tttatttatt ttaggtatgt tcatctctgg ctttggtatt tctttatata cattgtttaa
    12961 aaattcaact tttgatattt ctgcaagatt tctatcgttt gctgttgtag ctgttatttt
    13021 tatagttatc ttttttactg ctacttgttt accaagattt attactatat cgctattttg
    13081 aacatctgta tatgtttcta atgctgccct actatatgta gatgttgtag agttatcatc
    13141 tttactgata ttatcccctt cttttggagt atcttcatct gaattaggtt tatccattga
    13201 tgaattttct ttatcattat ttttatcttg attggtatta gagtttactt cttgtttatc
    13261 gctattttcc tgttgtttat cattattttc atctttatta tttgaattat tatcagtctg
    13321 atttttattt tcatctttat tatttacttc ttcttgattt acttcttctt gttcgttatt
    13381 tacttcctct tgttgctcct tatcttttaa gtttttcata tattctttat tttcataagg
    13441 aatctgttga acattaccat cttcgtcagt aacctcaact attccttttg ctggagcagt
    13501 accttctgga gcttttatat tgatgacttc catattaagt gcttctgata aagctatatc
    13561 cattactgct ggtttttcct cagatatttc atcatttttt gaagatattg taactccatt
    13621 attttctcca ctaaataaat tttctacatt accattaata tctacatcac tatttccttt
    13681 taattcaacc ttacttgtat ctactatagg attggtagat actatacttt ctaggctttt
    13741 tgcatcttca ctttgtccta aatttttctg tacataatct aagtcaacaa tatctacctt
    13801 tccatctcta tttaaatcaa attctctttt atttgaatct atattctcta aaaccttatt
    13861 ataatcactc ttatctactt taccgtctcc atttacatca cccattaaga ataattcata
    13921 atttaaaggt ttatttttat tatctacact tgctctattg cttaataaga ctctttgtga
    13981 ataacttgat atatcaatgt ctttaatttc tttgttctta aatccagctc cagagacttc
    14041 tatattgtat tttccaacag gtaaattatt aaatacaata ttataatatg atacattctc
    14101 atctttattt tttatttcac ttctacttgc agataatttt tttatagtat attctatatt
    14161 atctgtccca atatttactg ttcctgaatc tttttctcca agctttatta ttccactatt
    14221 gttactatca tcttttaatc gtaactgcat attagtcata ctttctgact cgctattttt
    14281 tattggcagt gaaaaattta aatctacttc aagattcccc cttaactttt ctgtaactga
    14341 tttttcaact gcccttagct tcccctctaa tataggttct gcaaaaacat ttagtggtgc
    14401 actacacata gatactgcta gtacacaaga tattgccttt ttcatgaaac aacaatcctc
    14461 ccttaataaa tctctaattt ttcatgaatt taaatactat aagtttcaga caataaaaaa
    14521 agacactcaa agagaatctt aattataaaa taaaaagact ttactctttt tagcaactgg
    14581 ctgatatggc tttaaaagct ttaatcccta tagttttgcg tcattagatt tccctaattt
    14641 tgccatttaa ttttcactac tttcttaccc attttatact atatttaaac tttttaatac
    14701 aattttttta cattaaatta taaaaaaata acatttatat taataaatta atataaatgt
    14761 tattttccat tataatttaa ttatttatat agcttcctta caagcataat tttgtgtata
    14821 ttttcttatt ctttatatct tccataaaat tcatgtgtag catatagatt cttatctcct
    14881 gttatagcta taccaaatcc cattatttca taccctttat gcaacatatt agttccttct
    14941 tctggatttg tcttccactc attaaaaata aagtttccta tatcatctgc atctctattt
    15001 gtatatacag gtttatctga aattttatat ttaattatat tttcaccttg aactgctata
    15061 tatccaggtt taatctcact ccaatctaag tatttcaaaa aagtagaata tgaatttttt
    15121 ccatttatcg tatgagataa agtcttctta tttgccatta aattagacca atcatttgct
    15181 agaccctcta atctgcttgc tacagataac gctcttactc cattatcttt tctatactgc
    15241 tttgtaagag catatacttt accctttatt ctctctttaa atgctctatc atttggattg
    15301 taatataacc cttgttgatt tggtgaagct tgatttatac attgcttgat aacattctga
    15361 tttatgcctc ctagtacagt caatttgtct gcaccactta aaatactctt gttactgcca
    15421 ttttcaacta aaactacagg agcatttttt gctattgtag aggcgaccaa agcgtctgtt
    15481 aattgcaagc ctttacttag ataaaattct ttactatttt tataaaactc ttgtataact
    15541 atcttattag tttcaaatct atctgtgcca cccaatctaa tagaatttgt gctcttaact
    15601 aattcgtcgc tgattatttc actagaacca agcgcataac tctttaagcc tgtcgtgtca
    15661 aatcccacac tatttccatc tataagtatt ataggtactc catctctagc agctactggt
    15721 gatgcactta tcgcatctgc ttctcctgta aatccattgg ctataattac tttatctatt
    15781 ggcttaattt ctttgatttt ttcagctatt tttatacttg tatcaaatct attttcacca
    15841 aatattcttt gtacaacctt ctttttggat aataactgat tctcaacatt tttacttatt
    15901 gttgattctg agcctattat atacacagtc tttgctttgt ttaatctttc catagttgtg
    15961 tttggaatct gattttgctt tgtcattaaa ataggcgcat ttaaggctcc agctaaacca
    16021 cttgcactta gaccatctgc taagctatta tctgagttga ttagtattgc tgtattgtag
    16081 ttttgcttat ctgctatttt tgctgctgtc tcatatctgt cattcccttt tatttgctca
    16141 actgatgtca acgcatttac cataggagta tttaccatta ctaaaaatag tgtaagtcct
    16201 atagctaata tctttttact attcttcata ttattttcta cccccaatgt acttttcata
    16261 ttttttatta gtactcatac ttatttttaa tcaaaagtta caattttgtt accatatttt
    16321 atatatgata ccatagttat ccatacaact caaacaatat acctaaagtt tttattctct
    16381 ataaattcaa aacattataa tttattttat ttaattaagt tataagttca aaataattta
    16441 tgttagtaat atgtttaatc attataagaa gtatcttttt ataataattt ttgcaatatg
    16501 aatttctcca cattataatc aaaaaataaa ataaggtatc tcaatctaat tctaaatgag
    16561 acaccttatt tttattattt tatcttgtta tttgtatatt ttataatatc tacactatgt
    16621 cttacatatt aacgcattgt ataatcatat attatctaaa taaactttta atattaattc
    16681 ctatatctta ttagttctta gtaaataagt ttattttttc tgctattttt tcaatagcgt
    16741 ttttagctat tccttcacct atttgtacta acttagtagc attcttaagt cttaactcaa
    16801 ctacttcttc ttgtgattta gaaagttctt ctgtagttaa tactattgga gcctttgaac
    16861 tagctgcaag tggtgctgct gtaagtgcat caactaattg tccatttcca cctacataac
    16921 catcttttgc tacatatgct ttagttgcac ctgcatagta tttattgatt actttagcat
    16981 tagtatcttt tctattttct ccagctaatc tttcaacttt attatttaaa tctatatcaa
    17041 ctaatttgtc ttcaacttct tttgatacag aattttctcc acccactatt tctaattctt
    17101 ttccttttaa taatttttca gcatttgcat ctaattcgtt acctgtaact attattggag
    17161 caccaaattg agcagctcta gctgatatac tcatagcatc agcttctcca tttccaccta
    17221 ctacaaaagc agtcttaaat gtatctttta attgttttgc tatttctaaa gaagtttcaa
    17281 atctatcttc tccagaaact ctctcaactt tacttacacc taatttagaa agtttttctt
    17341 cagtttcttt tgatacttta gattctccac ctactatata tattttctta gaagatagat
    17401 tacttccaag tactcttaat atttcagctt ctatttctga tggtagtgaa tcttttttag
    17461 ataataatat tggtgcattt ttttgagagg caagtggtgc tgatgctaat ccatcaacta
    17521 tagcatcttc tccaactaaa attatagctt ctgcttctcc atctttgaat ctctctttac
    17581 taacttctac tgctgttgta aatcttgaat caccagataa aacctctact tttaattctt
    17641 ttccatcttt tacatcagtt aatactttgt gtaagtctct aactaactta tggtttttac
    17701 ttgaaacact tattgtttct gacttttcat ttgccttgct taaaactata tcaaagcttg
    17761 atttttctgg tttatcttta tcatcactat cttcttgttc ttgagtaagg attgcttcat
    17821 tttcaaatgt taaagcatat ccatttttaa ctttattaac tatttctctt cctaatgtag
    17881 ttaatctaat tccatcaaat aattcttctg ctgatatgtc ttgtaaatct gcgtttttaa
    17941 ctgttatcgt atgtaattct tttgaatcta tgtcactata actcttttca taaccttcaa
    18001 aagtaccttc ttctgatgtt atcacttttg agaaatctaa atgttggtct cctacattta
    18061 atgtaataac atttgctgaa tcttttgctc tttttacttc aactgtattt gtagatttta
    18121 tagttgctgt taacttgtta tcagaatctt ctgctaaagt tgcattgtaa gcattaactg
    18181 catctactat ttcttgagca gttttgtagt tttcaatttt atagtcaact acttttccac
    18241 ctaacacttg atgtccttta tcttgaatag taacttttaa actttgccct tctgttaaag
    18301 agtttatttt attttgtaat tgagttgcat tagttaagtt tacattgtct acttttatat
    18361 cgtaaactct atcatttaca tttgctcctg cttttgtatc ttcaaactta acttctaatg
    18421 cttttctaac ctcttcgatt agtttagcag aatctttcgc acttactgtg taatttttat
    18481 caacattttc tgtcacagta ttgtctgcga atattggtgc tgctgcactc ataactgtag
    18541 cacctgccat tactattgcc atacttctct ttttgttgct taacatattt atgtcctccg
    18601 aatattattt tttatttttt atattcgtat ttcccacaca taaataatat tatatataat
    18661 attatttttc aatatatttt atgtgtttac ataatttact aacacaaaca ctaataatat
    18721 cttcttgaac cccttccccc tacttagctc tctataatta tgctcaaaga tagaaatccc
    18781 tattttatag tgattttcta ctaaacaatc atatactaac aaaagtattc aaactccatt
    18841 tttatatact actatgcact aatttttgct ttgctcttat tacttaaata tatatcacta
    18901 aattaatatc tacctccata
//
LOCUS       DACTGW010000062        18894 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.062, whole genome shotgun sequence.
ACCESSION   DACTGW010000062 DACTGW010000000
VERSION     DACTGW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.062"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..282
                     /locus_tag="I9573_001655"
     CDS             <1..282
                     /locus_tag="I9573_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796281.1"
                     /translation="RDIIIYFANQRESINIKKIIRMLENSAFSPNTDFQIERLIERLL
                     VEDFELLNSVSHIKSNKIFEDEMKINTEINLETMTAVKLEIANIKFEII"
     gene            300..1028
                     /locus_tag="I9573_001656"
     CDS             300..1028
                     /locus_tag="I9573_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796282.1"
                     /translation="MNISLNDYKIANEIFYRLLKKSELKEKEAKELFLAYINNSNVRY
                     ALEAICEVSKIKIILISDTLYLIPNNDNDIFGFDYRRESLLGKDLKDSYLGYLIMTII
                     FSEFTNGVAPASYIQVLNLLDLVQESLERSIARDNLEEIEKSCEFNITILKNYWESKR
                     KWDESSKKGIETTSADFQIGFIRKIINFLKNEGLIVHIEDEDKIKPTRRFLDLMNNYF
                     LDEERKVLIEKLVLRKGESINASN"
     gene            1015..5370
                     /locus_tag="I9573_001657"
     CDS             1015..5370
                     /locus_tag="I9573_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796283.1"
                     /translation="MPVINKYKIINFKFGMGQERILSNRTMELYGENLQVEAENTAGK
                     TMSIQTIIQSICPLSDVAKPFTAIYNKKEPLYSMTEWLLDDGKTLLLTGIGFEKKAGS
                     SKEEGKEKKDDLYKYFMFIIEESKEMEVDIDNIPLLKNNDKGIRIVQPLTASQDYIKM
                     LRDKYGKNKVHFFNNYLRKQHREKLEEYGINQSEWKEIIIKLNSNNKEGGLSDFVKEH
                     NTSEKLIRDKILPLIETSLTTEEFAPILQIKEQVRKCINNIIDMNEKLSDYKNYRDLN
                     IRVESLQNKINRILFIEEDKNNVLGELANLYVHIENKRNELNEEIKQYQKMKASKEEE
                     FKIVEYEEDSYEYFRKEKEIQDTQVEFDKAKNELNDKIEELELDEQYLNISKFKRKKK
                     GLEEGEKDKIEKESEKQRKEVNQEEITAIINRLGSSIHYCLKEESIINNEKLKENTNN
                     NIAIQEKKNQLNDDKSKLTKVLISKNSEIKYIEGKERDFNNQLHEFKLNNTDIKGFIS
                     NNFLGEVIDYTRYINYLNIKVKSIEENLESKVMQIEESNKLKDDINEAINNLKLDNHE
                     SYMKLQVLKTRKKDIDNFKEKYKIIKTRFEIDNELDNSTKLYKEVKQELNKMKLQVQD
                     LENNKKQLEKELEDLQNYKDIIFEEEILTDLERNGIKPIEGMKYLLTLDGDLEYKNNL
                     ISKNPLLPYSIIITNKEYQKLKQINLKNKTKISMPIFVRDNIEDTFITTSSNLVISNG
                     LNILSSFDNKALDPEKREKDMNLIKEQINSIRDKVEQYKAEIEILEEFKLLTNKIDFT
                     AEDLSIDVKIENYKKIIFEINNKIKSNENNEKNLSNKIKELYENKEKLLNNKNILFNK
                     LKDIRNIQDTYSIILENKNEITKLKTSIEKIEEEKKVIKKSIEDIDVNIFNLNSEKDR
                     INRHIDDTESKINKFKIYEPIKLENAKLEELEIKYKKYLEDPTKKEIETLNYEISVCL
                     KRIESITIELEEFRQLISSDNFENIPILFTIEELNKRIVLNKSKVDELRKLKYSIKSD
                     IDKNQGSLNTKREEILKKFNGKEPLSTSLIVNFNFEERKIDIKKDIKDIGEIIEGNRT
                     TCQNLEQELRDLKKYKVERKAITYQPEDIFIESKKLQSKLQNVEYNLIKSINSYKNTI
                     LEIKEFNYKHMEKYKNFIETLNVNKDNYSRQKEQVEIVLLVIREEISKLEKHSEQLRK
                     EKTIISRQIKDYVMDCIDEFKIINKLGKHKGQALFNISLPKSDKIENSLSLVDMLMDE
                     IVEEANLGDVEQKINTFYILNKTLNIGRIPVSVIKYEINRNETIKWNSINNETTGGQR
                     FCISFIVTILLMEYKRYDRNAIVDESKYKGKVLLMDNPFGETSQQDFLKEIFELAKKF
                     NVQIISYTHVTNASIRAMFNKIYLMTVEKTTSNKEFVDINEIKQNKTDELVYMSKFYI
                     GNKEELIQEDIFSLI"
     gene            5472..5681
                     /locus_tag="I9573_001658"
     CDS             5472..5681
                     /locus_tag="I9573_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796284.1"
                     /translation="MKNFVIVSTKAALLRVIIVRLLLKIGLKTYKYLSYFMKKEKLKR
                     IIRPEGCELRVNRSIQVERAIAEQI"
     gene            6077..6217
                     /locus_tag="I9573_001659"
     CDS             6077..6217
                     /locus_tag="I9573_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4796285.1"
                     /translation="MKIKIKKYREKLLMIQNELAKSVGVRRETIVHLENGLMYHKGIQ
                     DK"
     gene            6355..7317
                     /locus_tag="I9573_001660"
     CDS             6355..7317
                     /locus_tag="I9573_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HAT4796286.1"
                     /translation="MAKIDNMMSILWMLNSDKKVTAKQISEKLEINIRTVYRHIDALS
                     ASGVPIISDTGHNGGYTLLNNFIKAPLFFDTEEQTALLHAAIFAKEEGSFLDDTLDKA
                     TSKLKMYSNQKQEETLTKHLIGFEVIKPTGKISIESVLKKLKNSIANELSVEINYRTA
                     HEEKSKCRIINPYGIVRWNNNWYIVAFCQMRNEIRSFRVDRISSVIETSNTFNRPKEF
                     SASEFFIRGIMDETKDEDNLISLVIEGKMNALDNLCQHWLLGYYLKERTSNKAIFKLQ
                     EEIIFTYVPNILIPYRKSIQIIEPLSLRDKMVEDLLELINHYQI"
     gene            7395..8024
                     /locus_tag="I9573_001661"
     CDS             7395..8024
                     /locus_tag="I9573_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAT4796287.1"
                     /translation="MKKRKVYMYVFDTMSDWEVGYLTAELNTGRYFKKGLKPLKVITV
                     GVDKNPIITMGGLKVLPEITIDELNIESNDLLILPGGNTWTSIGHEKILEKSKEALEQ
                     RSIVAAICGATLGLAKTGLLDFRNHTSNDLEFLKMVILSYSGEKYYKMESAVNDENLI
                     TASGIAPLEFTVQVLKVLDVFKEEALSSWLNLYKTHDSKYFFELMNCNK"
     gene            8855..9271
                     /locus_tag="I9573_001662"
     CDS             8855..9271
                     /locus_tag="I9573_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796288.1"
                     /translation="MSKSETGVKSKLILELLVITYLFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSKFILSKVNQIAITIMMYSIVIIAIFFATPYISNI
                     NISNLDTGIILIAVLFIQSLSKLILYIYFDKRGICN"
     gene            9278..9484
                     /locus_tag="I9573_001663"
     CDS             9278..9484
                     /locus_tag="I9573_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4796289.1"
                     /translation="MSMKTKIKEYREKLLMTQNELAKSVGVRRETIVHLENGKYNPSL
                     KLAMDIAKVFGTTVENLFEFIEED"
     gene            10119..11162
                     /locus_tag="I9573_001664"
     CDS             10119..11162
                     /locus_tag="I9573_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAT4796290.1"
                     /translation="MKRKIAVVLLTVAMLLSLVACSNDNTSKSDKLEDTLVVYTTHGE
                     DMLGAISEEFTKETGVNVEFINLKGELADRVRSEKDNPQADIMFGGDTSTYMQLKEEG
                     LYEKTEPSWNNELDKSYKDSDGYWYGTIKTPVMMFYNTKLLSADKAPKDWSDLTKEEY
                     KDLIVTRDSLSSSMRSTISNLINYYSNTKSEEEAWKYLEDLNKNIKNYYNSGSVMYSA
                     IGKGEATISVAVLSDIIENKEKNNMPLEVVDAESGSVVITDCVAALKNAPHPNAAAKF
                     VDFVGSAKVQAMVANDFNRIPTLDSALKDSPKWMSKEYKAMDVDWSVIGKNQSNWIEK
                     WETDIIDNNKTIK"
     gene            11342..11968
                     /locus_tag="I9573_001665"
     CDS             11342..11968
                     /locus_tag="I9573_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796291.1"
                     /translation="MICRHSLSNKVNFLLCKTFNYSKSTIYMGKEIKMELKISDYKLL
                     CNKGSGAFNEDIVGLSPYGAWVLDGATGLNNKNLVSTESDAKWYVSWWNSYLHENMER
                     EDSLKVIIKEGIEEITKEYYKLTDDKEVEAIDIPSCSCSILKFYKDKIEYLLLGDCTL
                     LVNNNESVKILKDETLCELDKKVFDFMKEAKDATNLTLKERKAEVMRL"
     gene            12013..12297
                     /locus_tag="I9573_001666"
     CDS             12013..12297
                     /locus_tag="I9573_001666"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796292.1"
                     /translation="MEFSKEAVDNCVSGFIDIEYKMKIMMASDGFSCGYDRYKIFDKN
                     EMMKIAEIKGIDYIYRKVRELEKDDEMAIEFPRFKIYDDSSCVYLEIYKY"
     gene            12316..13308
                     /locus_tag="I9573_001667"
     CDS             12316..13308
                     /locus_tag="I9573_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796293.1"
                     /translation="MKNIELKEINHYYGKEKVLHDINISIKEGEFFTLLGPSGCGKTT
                     ILRIIGGFIKPSSGSIYVGDKNITNLEPENRNMGTVFQNYALFPNMTVEENVSYGLKI
                     KKLSKKIIKEKCDNYLELSGMKDFRKKKIDELSGGQQQRVAVARSLATEPTMLLLDEP
                     MSNLDIALRIKMREEIREIQQKTGITTLFITHDQQEALAISDRIAVMDKGKVLQIGVP
                     IEVYKSPLNDFVANFVGTSNCIGKEDYVNFNIEKETKPYIYKRPEEMILLQNTNQSGF
                     VKVKVESKNFLGAILEYTVSNDGKKYQVTELNRLNNSNKLNVGDMAYLGVLQGD"
     gene            13310..15004
                     /locus_tag="I9573_001668"
     CDS             13310..15004
                     /locus_tag="I9573_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAT4796294.1"
                     /translation="MIKKINRSKFTSKNVIFIVLYSILFWILIGYIIIPIGNTFVQAF
                     QGKGGYSFEVFKEYFSNTNNLRVVSNTIILGLGSVFVCGIMGISLALYMTFVCDKHKK
                     IIHILLLSPMMIPGVIMVIACIQIYGESGIVTKSIEILLPFIKKPYDFSGLKGILFVV
                     AYTQYVYFYLNVYVALKYVDYSTVEAAKGMGASKLRVFFDVIWPVITPAVITSTIITF
                     ASGISSFSAPNLIGGGFKVLSTQIVRSKANNHMDIASVQVITLLLMGILFMILLQYYG
                     KKYGVVPSLKSQSYKVHNRKKDLFTFVCKVIIEIQIIMIMLPIVTIVYLSFVSTHSIM
                     TTIFPRDFTLENYQMIFQKSRVLQPILNSFKMSLMAVAVGLIITVPVSYLVIKKDTAY
                     NRLAKFIIMLPWSMPASAIAVNLINTFNQKSIFAFNESLIGGFYIVPIAYTIYALPLL
                     FSSNEVAMKSINLGLEESSRSLGAGRIKTFYNVIVPNMMPGIISGAILVFIRTIGEYT
                     MSALLYGVYNRPISISIVTNMQEFKIGVSMAYGVLVIGICYIALGIIFKLDRKRFT"
     gene            15555..16433
                     /locus_tag="I9573_001669"
     CDS             15555..16433
                     /locus_tag="I9573_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAT4796295.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQGANEKNAPV
                     ILAIHPNELSFLGDSFVVSCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            16469..18364
                     /locus_tag="I9573_001670"
     CDS             16469..18364
                     /locus_tag="I9573_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAT4796296.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSETKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFPPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVAKPTIRSENS
                     EVELLGA"
     gene            18632..>18894
                     /locus_tag="I9573_001671"
     CDS             18632..>18894
                     /locus_tag="I9573_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAT4796297.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKS"
BASE COUNT         7922 a         1939 c         3145 g         5888 t
ORIGIN      
        1 agagatataa taatttactt cgctaatcaa agggaaagta tcaatatcaa aaagataatc
       61 agaatgttag aaaatagtgc cttctcacca aatacagatt ttcaaattga aagattaata
      121 gaacgtttat tagtagaaga ttttgaacta ttaaatagcg ttagccatat taaaagtaat
      181 aaaatatttg aagatgaaat gaaaataaat actgaaataa atttagaaac tatgactgca
      241 gttaagctag aaatagcaaa tataaaattt gaaataatct aaatataagg ggaaatacaa
      301 tgaatatatc tttaaatgac tataaaatag ccaatgaaat attttataga ttacttaaaa
      361 aaagtgagtt aaaagaaaaa gaagctaaag aattattttt agcttatata aataacagta
      421 atgtaaggta tgctttagag gcaatttgtg aagtatctaa aataaaaata atattaattt
      481 cagatacctt atacttaata ccaaataatg acaatgatat ttttgggttt gactatagaa
      541 gagaatcttt attagggaaa gatttaaaag atagttattt aggatatttg ataatgacaa
      601 taatattttc tgaatttaca aatggggtag caccagcatc atatattcaa gtattaaact
      661 tactggattt agtacaagaa tccttagaac gttcaatagc tcgtgataat ctagaagaaa
      721 tagaaaaatc atgtgagttt aatataacta tcttaaagaa ctattgggaa agtaaaagaa
      781 agtgggacga atctagtaag aaaggaatag aaacaacatc agcagatttc caaatagggt
      841 ttattagaaa aataattaat ttcttaaaaa atgaaggctt aattgtacac attgaggatg
      901 aagataagat aaaaccaact agaagatttc ttgatcttat gaataattat tttttagatg
      961 aagaaaggaa agtattaata gaaaaactag ttttaagaaa aggggaaagt attaatgcca
     1021 gtaattaata aatacaaaat tataaatttt aaatttggta tgggacaaga aagaatactt
     1081 agcaacagaa ccatggaact gtatggagaa aacttacaag tagaagcaga aaatacagca
     1141 ggcaaaacta tgagtattca aactatcata caaagtattt gtccactatc ggatgtagcc
     1201 aaaccgttta ctgcaatata taataaaaaa gaaccattat actcaatgac tgaatggttg
     1261 ctagatgatg gtaaaacgtt attattaacg ggaataggat ttgagaaaaa ggctggatca
     1321 tctaaagaag aagggaaaga aaaaaaggac gatttataca aatactttat gtttattata
     1381 gaagaatcta aagaaatgga ggttgatata gataatatac ctttattaaa aaacaatgac
     1441 aaaggtataa ggatagtaca acctttaacg gcctcacaag actatataaa aatgctaaga
     1501 gataaatatg gaaaaaacaa agtacacttc tttaataatt acttaagaaa acaacataga
     1561 gaaaaattag aagaatatgg aattaaccaa agtgaatgga aagaaattat tataaaactt
     1621 aatagtaata acaaagaagg tggactatca gactttgtta aagaacataa tacttcagaa
     1681 aaacttataa gagataaaat attaccttta atagagacaa gcttaacaac agaagaattc
     1741 gctccaatat tgcaaattaa ggaacaagta agaaagtgta taaataatat tatagacatg
     1801 aatgaaaaat tatcagatta taaaaactat agagatttaa acattagagt tgaaagttta
     1861 caaaataaaa taaataggat cctattcata gaagaagata aaaataatgt attaggagaa
     1921 ttagctaatc tatatgtaca tatagaaaat aaacgcaatg aattaaatga agaaataaaa
     1981 cagtatcaaa aaatgaaagc atcaaaagaa gaggaattta agatagtaga atatgaagaa
     2041 gatagctatg agtattttag aaaagaaaaa gagatacaag atactcaggt agaatttgat
     2101 aaagccaaaa atgaattaaa tgataaaata gaagaactag aattagatga acaatattta
     2161 aatatatcaa agttcaaaag aaaaaagaag gggctagaag aaggggaaaa agataagata
     2221 gaaaaagaaa gtgaaaaaca aagaaaagag gtaaatcaag aagaaataac ggctattata
     2281 aatcgtctag gtagcagtat tcattattgt ttaaaggaag aaagtattat aaataatgaa
     2341 aaacttaaag aaaatacaaa caacaatata gctatacaag aaaaaaagaa tcaacttaat
     2401 gatgacaaaa gtaagctcac aaaagtattg ataagtaaaa attcagaaat taaatatata
     2461 gaaggcaaag aacgtgattt taataatcaa ctacatgaat ttaaacttaa taacactgat
     2521 attaaaggct ttatttctaa taatttttta ggcgaagtaa tagattatac taggtatata
     2581 aattatttaa atataaaagt aaaatcaata gaagaaaatc ttgaatcgaa agtcatgcaa
     2641 atagaagaat cgaataaact taaggatgat ataaatgaag ctataaataa tcttaaatta
     2701 gataatcatg aatcttatat gaaactgcaa gtattaaaaa ctagaaagaa agatatagat
     2761 aattttaaag aaaaatataa aatcattaaa acaagatttg aaatagacaa tgaattagat
     2821 aactctacta aactatataa agaggtaaaa caagagctta acaaaatgaa acttcaagta
     2881 caggacttag aaaataataa aaagcaatta gaaaaagagc tagaagattt acaaaattat
     2941 aaagatataa ttttcgaaga agaaatatta acagatctag aaagaaatgg aataaaacct
     3001 attgaaggca tgaaatatct attaacctta gatggagatt tagaatacaa aaataatctc
     3061 atatctaaaa atccattgct accatattct ataataatta caaataaaga gtatcaaaaa
     3121 ctgaagcaaa taaacttaaa aaataaaact aaaatctcaa tgcctatatt tgtaagagat
     3181 aacatagaag atacttttat aactacatct agtaaccttg taattagtaa tggattaaat
     3241 atactatctt ctttcgataa caaagcttta gatcctgaaa aaagagaaaa ggatatgaat
     3301 ctaatcaaag aacaaataaa tagtattcga gataaagtgg agcaatataa agcagaaatt
     3361 gaaatattag aagaatttaa acttctaact aataaaattg attttacagc tgaagactta
     3421 tccatagatg tgaaaattga aaattataaa aaaattatat ttgaaataaa caataaaatt
     3481 aagagtaatg aaaataatga gaagaattta tctaataaaa ttaaagaact ttacgaaaat
     3541 aaagaaaagc tattaaataa caagaatata ttatttaata aattgaagga cattagaaat
     3601 atacaagata catattctat aattttagaa aataaaaatg aaataactaa gttaaaaaca
     3661 agtattgaaa aaatcgaaga agaaaaaaaa gttattaaaa aatcaataga agatattgat
     3721 gttaatatat ttaatttaaa ttcagaaaaa gataggataa ataggcatat agatgataca
     3781 gaaagcaaga taaataaatt taagatttat gaacctatta aacttgaaaa tgctaaacta
     3841 gaagaattag aaataaaata caagaaatac ttagaagacc ctacaaaaaa agagattgaa
     3901 acattaaatt atgaaatctc agtttgctta aaaagaatag agagtattac tatagagtta
     3961 gaagaattta gacaacttat tagtagtgat aattttgaaa atatacctat attattcaca
     4021 atagaagaac ttaataaaag aatagtttta aataaaagta aggttgatga actacgaaaa
     4081 cttaaatata gtattaaaag cgacatagat aaaaatcaag gttcattaaa cactaagagg
     4141 gaggagatac ttaaaaaatt taatggaaaa gaacctttat ctactagctt aatagtgaac
     4201 tttaattttg aagaaagaaa aatagatata aaaaaagata taaaggatat tggtgaaatt
     4261 atagaaggta atagaactac ttgtcaaaat ctagaacagg aattaagaga cttaaagaaa
     4321 tataaagtag aaagaaaagc tataacctat caacctgaag atatttttat tgaaagtaaa
     4381 aaactacaat caaaactgca aaatgttgaa tataatttaa ttaaatctat aaatagctat
     4441 aaaaatacaa tattagaaat aaaagaattt aattataagc acatggaaaa atacaaaaac
     4501 tttatagaaa ctttaaatgt aaataaagat aattactctc gacaaaaaga acaagtagaa
     4561 atagtattac tagttataag agaagaaata agcaaattag aaaaacatag cgaacaacta
     4621 agaaaagaaa agactataat atcaaggcaa ataaaagatt atgttatgga ctgtattgat
     4681 gaatttaaaa tcataaataa acttggtaaa cataaaggac aagccttatt caacatatca
     4741 ctacctaaat ctgataaaat agaaaatagt ttgagcttag tagatatgct aatggatgaa
     4801 attgtagaag aagcaaacct aggtgatgtt gaacaaaaaa taaatacctt ttatatttta
     4861 aataagactc ttaatatagg gagaattcct gtatcagtta taaaatatga aataaataga
     4921 aatgaaacta taaagtggaa ctctattaat aacgaaacaa caggaggaca gagattttgt
     4981 atctcattta ttgtgacaat attgttgatg gaatataaaa gatatgatag aaatgcaata
     5041 gttgatgaaa gtaaatataa gggtaaggta ctacttatgg ataatccttt tggagaaacg
     5101 tcgcagcaag atttccttaa agaaatattt gaacttgcta aaaaattcaa cgttcaaatt
     5161 atatcctata ctcatgtaac aaatgcttct ataagagcaa tgtttaataa gatttattta
     5221 atgacagttg aaaaaactac ttctaataaa gaatttgtgg atattaatga gattaagcaa
     5281 aataaaactg atgaattggt gtatatgagt aaattttata taggtaataa agaagaatta
     5341 attcaagagg atatatttag tttaatataa ataacaagta aacaaataaa gtgattacta
     5401 tcagtgaggc aggtagaaac tgaattactt tgtgactcat tacgactctt ggggtaaaat
     5461 tgtgtataaa tgtgaagaat ttcgtgattg tcagtacaaa agcagcttta ttaagggtaa
     5521 taatagtaag actcctcttg aaaataggac taaaaactta caagtatcta agttatttca
     5581 tgaaaaaaga aaaattaaag agaattataa gaccagaggg atgtgaactt agagtgaata
     5641 gaagtataca agtagaaaga gctatcgcag aacaaattta attttatcat acgatagcct
     5701 cttgttattt tagtgatgaa atccatcaag cataccataa ctacagaaaa agaggctttt
     5761 ctgatggtat attgggttct tttagagtgt gtgatttgct acatgttcat taggataata
     5821 atagtcagta aatgatatta ctgatagaat agatataata aactaatcta gttccatatt
     5881 gtaatgttta ttaaagagta tacttttatg attataatag ctattttctt tgaagcttat
     5941 tgtacaagag atttgaatat atcaaatttg gatataggta taattctctt agcagtttta
     6001 tttatacagt ctttatctaa attagtgttg tatatacttt gataaaaggg ggatatgtaa
     6061 ctaagttgta atattgatga aaataaaaat taaaaaatat agagaaaaac tccttatgat
     6121 acaaaatgaa cttgctaaat cagttggggt aagacgagag actattgtac acttagaaaa
     6181 tggattaatg taccataaag gtatccaaga taaatagtta aaaaactaga taagaataag
     6241 gagcctttac agaacaaatt tagtgtgctc gtgcgatagc ccttttttta taaaaattaa
     6301 tctaataacc tatataatta aatttataaa acatatatga aagggtaaaa ctatatggct
     6361 aaaattgata atatgatgtc catattatgg atgttaaact cagacaaaaa agttaccgca
     6421 aaacaaatat ctgaaaaact agaaataaat ataagaactg tctacagaca cattgatgca
     6481 ttatctgcta gtggagtacc tataatttca gatacaggtc ataatggtgg atatacactt
     6541 ttgaataatt tcatcaaagc acctcttttt ttcgatacag aagaacaaac tgcgttactt
     6601 catgcagcta tatttgctaa agaagaggga agtttcttag atgacacact agacaaagca
     6661 acctcaaagt taaaaatgta ttcaaatcaa aaacaagaag aaactttaac aaaacattta
     6721 ataggttttg aagtaataaa accaactgga aaaatatcca tagaatctgt acttaaaaag
     6781 ttaaaaaata gtatagcaaa tgaattatct gtagaaatca actatcgtac agctcatgaa
     6841 gaaaaatcaa aatgtaggat tataaatcca tatggaattg tacgttggaa taataattgg
     6901 tacatagtag cattttgtca aatgagaaat gagattcgta gttttagggt agatagaatt
     6961 agtagtgtca tagaaacatc aaatactttt aataggccaa aagaattttc agcaagtgaa
     7021 ttttttataa gagggattat ggatgaaaca aaagatgagg ataatcttat atcattagtt
     7081 atagaaggaa agatgaatgc attggataac ttatgtcagc attggcttct aggatattat
     7141 ttaaaagaaa ggacttcaaa taaagcaata tttaaacttc aagaagagat aatatttaca
     7201 tatgtaccga atatacttat accttataga aaatctatac aaataattga accattaagc
     7261 cttagggata aaatggttga agatttactc gaattaatta atcattatca aatttaataa
     7321 cttaactgac agagtatgtc agttaagtta ttttacaatt aggtatatta attaattata
     7381 gaaggagcaa aaaaatgaaa aagagaaaag tatatatgta tgtattcgat actatgtcag
     7441 attgggaagt tggatatcta actgctgaat taaatacagg aagatatttt aaaaaaggat
     7501 taaaaccact aaaagtaatt acagtaggag ttgataaaaa tcctattata acaatgggtg
     7561 gattaaaagt tttacctgag attacaattg acgagcttaa tattgaaagt aatgacttat
     7621 taattttgcc agggggaaat acttggacat ctataggtca tgagaaaata ttagaaaaat
     7681 ctaaagaggc tttggaacaa aggtctatag ttgcagctat ttgtggtgct actttagggt
     7741 tagctaaaac aggtttacta gattttagaa atcatacaag taatgattta gaatttttaa
     7801 aaatggtgat actaagttat tctggagaaa aatattataa aatggaatct gcggttaatg
     7861 atgaaaattt aattactgct tctggaattg caccattaga atttacagta caagttttaa
     7921 aagttttaga tgtttttaaa gaagaagcat taagttcttg gttgaacctt tataagacgc
     7981 atgactctaa atattttttc gaactaatga attgtaataa ataaatttaa gatttaattt
     8041 taaattcaaa ttttaaatta ctttttctat ggcaatagat aagtagttta gaattttatc
     8101 atatatataa tttgtattta taccaacaca aatagaaatg atagttccaa taataaaatt
     8161 tataattagt tccatatgga gtacatttct ttcctactaa tataactaag aatatatgaa
     8221 accaccatag attgtagttt tttctgttgt attatatcat gggatagtaa agtgacgtat
     8281 atttttgatg aaaacctagg attgtatatg aaatgaatta atcctgtgta ttaatcttga
     8341 gattcgtgtc taatgatttg taaaaataaa taatgaaata cttagaaata cttgtaaatg
     8401 ctagtcaata ttggctagta ttttttattt tagtaatgaa atccccaact gtacttcatc
     8461 cacaaaaaaa gagatatttt tgagggtgta ttggagtgaa cttaggctgt ataattatac
     8521 atttcaatat cttatattaa ttaggataat agttggtaaa tgatatgatt gaaacaatag
     8581 atataataaa ctaatctagt actgtactgc ttactaaaga atatattttt atggtataag
     8641 aattttttag agcgttgatt ctataggagt gaataaaaaa gtaattttat aatattattt
     8701 gtaaccatta gatgtctatt atgaaatctt ttatggacta tatttgtaaa aatattatta
     8761 agtatttaca atattttctt gcaatttatt tggaaaggtt atatatttat aacatgaagt
     8821 gttataaata tataaccttt aaggtggatt ttatatgtct aaatcagaaa ctggtgtaaa
     8881 atcaaaactc atattggaat tacttgtaat aacttatctt tttatagctg tattagctac
     8941 taagtatatt tttaaaggtc caagtactgc atctcttcca ggactgagct ggttaatagt
     9001 ctttggatgc tattcaattt ataataaaaa atataatgat ttagttgatg aatcatctaa
     9061 atttatacta tcaaaggtta atcagattgc aataacaata atgatgtatt ctatagttat
     9121 aatagctatt ttctttgcaa caccttatat aagtaatata aatatatcaa acttggatac
     9181 tggtataatt cttattgcag ttttatttat acaatcgtta tctaaattaa ttttgtatat
     9241 atactttgat aaaaggggga tatgtaacta agtggcaata tcgatgaaaa caaaaattaa
     9301 agaatataga gaaaaactcc ttatgacaca aaacgaactt gctaaatcag ttggggtaag
     9361 acgtgagact attgtacact tagaaaatgg aaaatataat ccttctttaa agttagcgat
     9421 ggatattgct aaagtattcg ggaccacagt agaaaatttg tttgaattta ttgaagaaga
     9481 ttagtagata ggatataaat atttataaga ttgttaataa aatatagtta tttaattatt
     9541 aattttgtta aaaatattta taaataatat aatgtatgag taaagttagg ttgtaattat
     9601 tttaagataa gaagactact gtaaatgata aaaattagtg aatatacagc caattaataa
     9661 ataatagata aaactttaaa gcttagatgt gaggatgaga tatggtctta cagtagttca
     9721 cacttttaat taagacttaa aatggaaacc atacacatac atacactatt tactgaaagt
     9781 ggactagata aaaaaataat aagtgattta agaaagtatc tcatatacta tataagtatt
     9841 attttaggtg acctatttat aggtcatttt ttatgtatat aaatgataat aatactaata
     9901 tttaaaatag gttttaaaga aataaaatat ccagattgaa aaatggatta atgttaataa
     9961 aaaaattata tgtataaaaa taggtataat tatagaaaaa aacaataata aaataagctg
    10021 ttatagaaaa atacaattat atagattgtt gttgaatcat tgactgcaag aataataaca
    10081 tttaaaatga acatggagaa tatgggggag gaatcaatat gaagagaaaa atagcagttg
    10141 ttttattaac tgtagcaatg ttattatcac ttgtagcttg tagcaatgat aacacatcaa
    10201 aaagtgataa attagaggat acattagtag tatatactac acatggagag gatatgctag
    10261 gagcaatttc tgaagaattt acaaaggaaa caggagttaa tgttgaattt ataaacttaa
    10321 aaggtgaact agcagaccgt gttagaagtg aaaaagataa tccgcaagca gatataatgt
    10381 ttggtggaga tacatctaca tacatgcaat taaaagaaga aggattatat gaaaagactg
    10441 aaccaagctg gaataatgaa ttagacaagt cttacaaaga ttcagatgga tattggtatg
    10501 gaactataaa aacaccagta atgatgtttt ataacacaaa actattatca gctgataaag
    10561 cgccaaaaga ttggagtgat ttaacaaaag aagaatacaa agacttaata gttactagag
    10621 attcattatc atcatctatg cgttctacta ttagtaactt aatcaattac tactcaaata
    10681 ctaagagtga agaagaagct tggaagtatc ttgaggattt aaataagaat ataaagaatt
    10741 actacaatag tggaagtgtc atgtattcag caataggtaa aggtgaagct acaatatctg
    10801 ttgcagtatt aagtgatata atagagaata aagaaaaaaa taatatgcca ttagaagttg
    10861 tagatgcaga gagcggttct gtagtaatca cagactgtgt agcagctcta aaaaatgcac
    10921 cacatccaaa tgcagcagct aaatttgtag attttgttgg aagtgctaag gttcaagcta
    10981 tggttgcaaa tgattttaat cgtattccaa cattagattc tgcacttaaa gattcaccaa
    11041 agtggatgtc aaaagaatat aaagctatgg atgtagattg gtctgtaatt ggaaaaaatc
    11101 agtctaactg gattgaaaag tgggaaacag atataataga taacaataaa actataaaat
    11161 agcctaatta aataatttaa tattatatac aaaaatagtg aggattttcc tcgctatttt
    11221 acgtataagg aaactatagt atctcataat tttgtataat tcatacaaca aaatgatact
    11281 aatacatact tattaaataa aatagttttt gagtaactga tgaagtaatt gactatataa
    11341 agtgatttgt agacatagtc tctcaaataa agtgaatttt ttattatgca aaacttttaa
    11401 ttatagtaaa agtacaattt atatgggcaa ggagataaaa atggaactaa aaattagtga
    11461 ttataaatta ttatgtaata aaggtagtgg cgcttttaac gaagatatcg taggtttatc
    11521 accttatggg gcatgggtac ttgatggagc tacaggttta aataataaaa atttagtctc
    11581 tacagaaagt gatgccaaat ggtatgtaag ttggtggaat agttatcttc atgagaatat
    11641 ggaaagagaa gatagcttaa aagtaattat taaagaaggc attgaggaga taacaaaaga
    11701 gtactacaaa ttaacagatg ataaagaagt agaagcaata gatattcctt cttgttcatg
    11761 ttctatatta aagttttata aagataagat agagtattta cttttaggag attgtacttt
    11821 attagttaac aataatgaat ctgtgaaaat tttaaaagat gagactttat gtgaacttga
    11881 taaaaaagta tttgatttta tgaaagaagc caaagatgct acaaacctta ctttaaagga
    11941 gagaaaagcc gaggtaatgc gattataata gagaatagac ttaagaaaaa tactgtgggg
    12001 ggatattgga ctttggaatt ttctaaagag gccgttgaca attgtgtaag tggatttatt
    12061 gatatagaat ataaaatgaa aattatgatg gctagtgatg gtttttcatg tggatatgat
    12121 agatataaga tttttgataa aaatgaaatg atgaaaatag cagagattaa aggtatagat
    12181 tatatatata gaaaggttag ggaactagaa aaggatgatg aaatggcaat agagtttcct
    12241 agatttaaaa tctatgatga ttcatcatgt gtatatttag agatttataa atattagata
    12301 atttatggag acgatatgaa gaatatagaa ttaaaagaaa ttaatcatta ttatggaaaa
    12361 gaaaaagtat tacatgatat taatatatct attaaggaag gagagttttt tactttacta
    12421 ggtccttctg gatgtggaaa aacgactatt ttaagaataa ttggtggatt tataaaacct
    12481 tcttctggaa gtatttatgt tggtgataaa aacattacca atttagaacc agaaaataga
    12541 aatatgggaa ctgtttttca aaactatgca ctgtttccaa atatgacagt tgaagaaaat
    12601 gtatcttatg gattgaaaat aaaaaagctg tctaaaaaaa ttataaagga aaaatgtgat
    12661 aattatttag agctgtcagg aatgaaagat tttaggaaaa aaaagattga tgaattatct
    12721 ggtggacagc aacaaagggt ggcagtagca aggtcattag cgactgagcc tactatgctt
    12781 ttattagatg agccaatgtc taatctagat atagctttaa gaatcaagat gagagaagaa
    12841 ataagagaga tacagcaaaa gacaggtatt actactttat ttattaccca tgatcaacaa
    12901 gaagccttag ctatttctga tagaatcgct gtaatggata agggtaaagt tcttcaaatt
    12961 ggtgtgccaa tagaagttta caaaagccca ttgaatgatt ttgtagcaaa ctttgtaggt
    13021 acttcaaact gtattggaaa agaagattat gttaatttta atattgaaaa agagacaaag
    13081 ccttatattt ataaaagacc tgaagaaatg atattattgc aaaatacaaa tcaaagtggt
    13141 tttgtaaaag taaaagtaga atctaagaat tttctaggag caattttaga atatacagta
    13201 tcaaatgatg ggaaaaaata tcaagtaact gagcttaata gactgaataa tagcaataaa
    13261 ttaaacgtag gggatatggc atatttaggt gtgctacaag gtgactaata tgattaaaaa
    13321 aataaaccgt agtaaattta catctaaaaa tgttattttt attgttttat acagtatatt
    13381 attttggatt ttgattggat atattatcat accaataggc aatacttttg tacaagcatt
    13441 ccaaggtaaa ggtggatatt cttttgaagt ttttaaagaa tatttttcaa atactaataa
    13501 tttaagggta gtaagcaata ctataatttt gggattgggt tctgtgttcg tatgtggaat
    13561 tatgggaata tcacttgcct tatatatgac atttgtttgt gataaacata agaaaatcat
    13621 acatattctt ttactaagtc caatgatgat tcctggagta attatggtaa ttgcatgtat
    13681 tcagatttat ggagaaagtg gaatagttac aaaatctata gaaattttac tcccctttat
    13741 caaaaaacct tatgatttta gtggattaaa gggaatttta tttgtggttg cttatactca
    13801 gtatgtatat ttttatttaa atgtatatgt tgccttaaaa tatgtggatt actcaacagt
    13861 tgaagctgca aaaggaatgg gagcatcaaa gctaagagta ttttttgatg taatatggcc
    13921 tgttattaca cctgctgtta taacttctac tattataact tttgcatcag ggattagttc
    13981 tttctcagct ccaaatctta taggtggtgg ttttaaagtt ttaagcactc aaattgttag
    14041 gtctaaggct aataaccata tggatatagc ttcagttcaa gtaattactt tattactaat
    14101 ggggatttta tttatgattt tattacagta ttatggcaaa aaatatggag tagtgccatc
    14161 cttaaaatct caaagttata aagttcataa tagaaaaaaa gacttattta catttgtatg
    14221 taaggttatt attgaaatac aaattattat gattatgctt cctatagtta ctattgtgta
    14281 tttatccttt gtaagcacac attcaattat gacaaccata tttcctagag actttacact
    14341 ggaaaactat cagatgattt tccaaaaatc acgtgtatta cagcctatat taaatagttt
    14401 caaaatgtct ttaatggcag tagcagtagg attgataatt actgtaccag tatcatattt
    14461 agtaattaaa aaggatactg catataatcg actagcaaaa tttattataa tgttaccttg
    14521 gagtatgcct gcaagcgcca ttgcagttaa cctaattaat acatttaatc aaaaaagtat
    14581 atttgctttt aatgaatcct taataggagg tttttatatt gtacctatag catatacaat
    14641 ttatgctcta ccattattat tttcatccaa tgaggttgct atgaagtcaa taaatttagg
    14701 actagaagaa tcatcaagga gtttaggtgc tggaaggata aaaacctttt ataatgtaat
    14761 agttccaaat atgatgccag gaataatttc aggagctata ttagtattca ttcgtacaat
    14821 tggggaatat acaatgtcag ccttgcttta tggtgtttat aatagaccta tttcaatttc
    14881 tattgtaact aatatgcaag aatttaagat aggagtatct atggcttatg gtgtattagt
    14941 aattggtata tgctatatag cattaggaat aatttttaaa ttagatagaa aaagatttac
    15001 ataagataac aaaggctaat ttataaattg gtgcttagaa aaccaaatca ctttattagc
    15061 taaagatgaa tttgaagggt gacactttgg tgttttatgt aaagtttatg gaagtaaaac
    15121 attaaataat cgtgagtatg attcctataa atcgtatata ctattgaaat ccaagaaaag
    15181 gtattggcta aatagctaat atctttttaa ttttttgact cccatatagc attaactatt
    15241 acatgagtat ctttaaattt atagttaaag tatcttaatt gtttattata aaaatatagg
    15301 atggctaaag cacattaaat taaaacactt aaaaagagaa gatgtacatg agaaatataa
    15361 ttaactatga tacagacttc taaaaattat ttagtgaaaa ggaacttaga gtgtataata
    15421 aaagttcctt ttcaataagg aaaacgattg ttattttctg agcttcatta ccctgatatt
    15481 atttaaatat aaacaacaac aaaattaaca agaagtaaat tcaacctaaa atttaaaata
    15541 tcgggaggta caatatgtta atcaatatga aggaaatgct aaaagtggca caagaaaatc
    15601 aatttgctgt accagcattt aatataggaa gtgggcaaat acttaaagca gtagtacagg
    15661 gtgctaacga aaaaaatgca ccagtaatac ttgcaataca cccaaatgag ctaagttttc
    15721 taggcgatag ctttgtagtt agttgcatag aagaagcaaa taagagtaaa gtacctatgg
    15781 taatacactt agatcatggg gaaaataaag aacaaatatt aagggcaata agatgtggat
    15841 ttacatcagt aatgattgat ggctcacatt taccatatga agagaatgta gctatatcta
    15901 gggaaatagt agaaatagca aaaggattaa atgtgtccgt agaaggagaa cttggaacaa
    15961 taggaacaac aggaacatca agtgaaggtg gtactgatga aataatatat acagattcaa
    16021 aattagctaa ggattttgtt gaaaaaacag gagtagacac actagctatt gcaattggta
    16081 cagcacatgg tatatatcca aaaggtttta aaccagagct taaactagat ttattaaaag
    16141 aaataagaga agtagtagat atacctttag ttcttcatgg tggttcatca aatccagatg
    16201 aagaaatagc acaagcagtt aaactaggag tttgtaaaat aaatatttcg agtgatgtta
    16261 agtcggcata ttataagaag tgcagagaac tattagaaca aaacccaagc ttatatgagc
    16321 cagatactat atatccacca tgtataaaag ctgcaagaga agttatagag tttaaaatga
    16381 atctattcaa tgctatagat aagttaaagt gcttttataa aaataaaaat taattatgag
    16441 gtaacaaatt aaaaggaggg gagaatttat gataggtaaa atactacgaa gcgattgtat
    16501 tgtactagat aaacaattac aaacaaaaaa agatgtaatt aattatatgg caaatcattt
    16561 gtaccatgga aattatataa ataataaaga aaagtttata gctgatatta ataaacgtga
    16621 aaaagaatca tcaactaata tgggtaattt aatagctata cctcatgcta agtcaaatgt
    16681 agtaaatcaa cctgtaatga tgtatttaag aacagataga gagattacag ataatgatgg
    16741 tcaaaaactt aggtcatttt ttatgatagc agttccagaa tcaggttcag atgaacatct
    16801 taagattata tctaaactag ctacatatct aatggaagaa aattttctta agcatctact
    16861 aagtgcagaa actccatacg aaatattaag tatatttaat aataaagctg cagaatcagg
    16921 agattttagt aataatcaaa ataaaggtct tatagttggg ataacaggat gtccaacagg
    16981 tatagctcat acttttatgg caaaaaaagc attagaggaa gcagggcaag aacttggata
    17041 tgaagttaaa atagaaactc atggagctat aggtgtagaa aataagttga ctaaagaaga
    17101 tataaaaaga gctgaatacg taataatagc ttgtgataaa gaagttcaac tgagtagatt
    17161 tataggaaag aaagtgctac aaaagaaagt agtagatgct aagaatgttt cattagctaa
    17221 aaatttaatt ataaacgctg aaaatggaat ggctaaagaa attaaaccag gtggaaagac
    17281 tttagaagat tcaaaagaaa tgcttcaagg gtcagagaca aagggtgttt ataagcactt
    17341 aatgagtggt gtatctgaaa tgattccatt tgtagtgata ggtggaattg gtatagccat
    17401 agcatttatg tttggtatat ttgcatcaga cccaactcat gaaacataca accctatagc
    17461 tggattcttt ggacaacttg gaggcgattc agcatttaag ttatttatac cgatacttgc
    17521 tggttttata gctaaatcaa tagcaggaag acaaggattt cctcctgcta tgatagctgg
    17581 ttttatggca gttattggag gttcagggtt tataggtggt atggtagctg gttttgcaac
    17641 aggatatata gctttaggag taatgaagat aacagaaaat ttaccagatg tattaaaagg
    17701 agtaaatgct gtattgatat gtccactttt gattgcattt acgagtggag ctataatgtt
    17761 ctttgtagtt aatacgccag catcatggtt aaatgaagct ttacaatctt ggttacaaag
    17821 tatgtctgga actaataaag tattattagg tgcaatatta gcagggatga tggcatcaga
    17881 tatgggagga cctattaata aaacagcttc agcttttgga ttggctatgt tttcaagtaa
    17941 tatatttgag ccatcagcag cattaatggt aggaggaatg gtacctccat tagcgatatc
    18001 attagctatg ttaatattca aaaataagtt tactaaagaa gaaagaaagt catctctacc
    18061 aactgctata atgggggcat ctttcataac agaaggcgca ataccgtttg cagctgcaga
    18121 cccattaaga atgattccag caaatataat tggttcttgt ataggtgggg caatctgtat
    18181 gagtttagat ataactttaa tggcaccaca tgggggaata tttgtaatac catttgcatg
    18241 tagtgcacca gttgcatata tagcttgtat attgattggt acagtaataa cagcattgat
    18301 tataggggta gcaaagccta ctataagaag tgaaaacagt gaagttgaat tacttggtgc
    18361 ttaaattata aataatagta aaaataagta atggaataac aacaagtata tagaataata
    18421 ataaggatat ttagaatcag ttaactactt ctaaatatcc ttaattttta ttttgaaatt
    18481 atattatcta ttatatcaat ttagtaaggt gtatcataaa ttcttttatg atacagaagt
    18541 atcttagtaa ttttacatag ttagtaaata tgttaaaata gtacttaata taaacataga
    18601 aatatataaa cataggtagg gagggctaag tatgcttgtg tcgaggcaaa ttgaatttct
    18661 taaattgata ctaaacgaaa ataaatatag acctataaaa tactttaaag ataaattaaa
    18721 tgtatctgat aaaactttac aaaaagattt gaagatgatt gagaaatatt tagaaacttt
    18781 taatataaaa attgatataa aaagaggcta tggaatatta atagaagatt tagcaaagaa
    18841 gaacatagac ttaattaata gtttaaatat acaatcgaag gaaaataaat cttt
//
LOCUS       DACTGW010000063        18622 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.063, whole genome shotgun sequence.
ACCESSION   DACTGW010000063 DACTGW010000000
VERSION     DACTGW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18622
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.063"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(184..2850)
                     /locus_tag="I9573_001672"
     CDS             complement(184..2850)
                     /locus_tag="I9573_001672"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAT4796298.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKAHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKVKKEKFEEMIKSVEERLTNLESKIK"
     gene            complement(3489..4316)
                     /locus_tag="I9573_001673"
     CDS             complement(3489..4316)
                     /locus_tag="I9573_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAT4796299.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(4364..5800)
                     /locus_tag="I9573_001674"
     CDS             complement(4364..5800)
                     /locus_tag="I9573_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HAT4796300.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(5887..6756)
                     /locus_tag="I9573_001675"
     CDS             complement(5887..6756)
                     /locus_tag="I9573_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796301.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGNYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(6914..7567)
                     /gene="phoU"
                     /locus_tag="I9573_001676"
     CDS             complement(6914..7567)
                     /gene="phoU"
                     /locus_tag="I9573_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAT4796302.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(7595..8359)
                     /gene="pstB"
                     /locus_tag="I9573_001677"
     CDS             complement(7595..8359)
                     /gene="pstB"
                     /locus_tag="I9573_001677"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAT4796303.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(8378..9202)
                     /gene="pstA"
                     /locus_tag="I9573_001678"
     CDS             complement(8378..9202)
                     /gene="pstA"
                     /locus_tag="I9573_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAT4796304.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVIIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(9204..10121)
                     /gene="pstC"
                     /locus_tag="I9573_001679"
     CDS             complement(9204..10121)
                     /gene="pstC"
                     /locus_tag="I9573_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAT4796305.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(10503..11738)
                     /locus_tag="I9573_001680"
     CDS             complement(10503..11738)
                     /locus_tag="I9573_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4796306.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DNNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDIYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAITAPTF
                     SGEYQTQSDSILRCTKHGHTRLAFTKILALFSIFIVMFVVCISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGLKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            complement(11739..12626)
                     /locus_tag="I9573_001681"
     CDS             complement(11739..12626)
                     /locus_tag="I9573_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796307.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYIMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            complement(12771..13886)
                     /locus_tag="I9573_001682"
     CDS             complement(12771..13886)
                     /locus_tag="I9573_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4796308.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            complement(13922..14620)
                     /gene="vanR"
                     /locus_tag="I9573_001683"
     CDS             complement(13922..14620)
                     /gene="vanR"
                     /locus_tag="I9573_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family DNA-binding response
                     regulator"
                     /protein_id="HAT4796309.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            complement(15002..15682)
                     /locus_tag="I9573_001684"
     CDS             complement(15002..15682)
                     /locus_tag="I9573_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4796310.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKEISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYMTYINEEQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(15732..17747)
                     /locus_tag="I9573_001685"
     CDS             complement(15732..17747)
                     /locus_tag="I9573_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAT4796311.1"
                     /translation="MKKYNILKGTLIVLIVSLLAIAGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGEDKLLSLELPEIFSASEVWINGEIVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDSSY
                     FMVVLCIICINGSISKIEEDKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFIIVILFSLMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQSFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKVSDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(17819..18496)
                     /locus_tag="I9573_001686"
     CDS             complement(17819..18496)
                     /locus_tag="I9573_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4796312.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVERI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
BASE COUNT         5953 a         3335 c         2012 g         7322 t
ORIGIN      
        1 cataatatat caaattttta ttgcttataa attttaagac ataaaattca tatattcaca
       61 acataaaata aaaatataac acatctgtaa agaattataa catcatattt gtatatatat
      121 aaagaaaaac atacaaaaag tatagaatat atactttttg tatgttttta ctaattctta
      181 tatttatttt attttagatt ctaaatttgt taatctttcc tcaacagatt taatcatctc
      241 ttcaaatttt tcttttttta ctttttcttc ttcaattaaa ctttcaggag cttttgctaa
      301 gaaaccttga tttgcaagct ttccattaac tctctttatt tcgccttcta actttttctt
      361 ttctttagac aatctctcaa tttctttttc aaaatcaact aattcattaa gaggtataaa
      421 tatttcaaca ccatcaataa ctactccaac tgcatcttct ggaacattag atttatcttt
      481 tgctatctct acatttgaag cagatgctag agttataaaa tagtctttac caagctcaat
      541 agcttctatt ttttcttcac ttgggattat tatcaattta gctttcttag aaggtggtac
      601 attcatttct gctctcacat ttcttatact tcttatacct tccatcaata agttcatcat
      661 atcttcttct tcagccatgt tatcttcttc attatatttt ggccattcag atactattat
      721 acatccttca acagtaggaa gatgagtata tatttcctct gttataaatg gcatgtatgg
      781 atgtaataat tttaatattt tttcaagaac ataagttaat gtatataatg ctgcagattt
      841 agcatttgca tcttccccat ataatctagg tttaaccatt tcaatatacc agtcacagta
      901 ttctgaccaa gtaaaatcgt atatcttttg aagtgctatt cctaaatcaa acttatccat
      961 gttattagtt gcttctttta caatattatt tgctcttgat attatccatt tatcagctag
     1021 agttaagtcc tctttaacac tttctctagt catgttttta attatatctt catcaatatt
     1081 cataaataca aatcttgatg cattccacaa cttatttgca aagtttctag caaattctac
     1141 tctttccata tagaatctca tatcatttcc tggggaattt ccagttgtta atgtaaatct
     1201 tagggcatca gctccatatt gctcgattat ctccaatggg tctataccat ttcctaaaga
     1261 tttactcatt tttctacctt gagaatctct tactaatcca tgaaccaaaa catggtcaaa
     1321 tggcttttca ttcatacaga acatacctgc aaatgccatt ctaactaccc agaagaatat
     1381 tatatcgtat cctgtaacaa gtacacttgt tggataataa taatctaaag cttcagtctt
     1441 atttggccat cctagagtag agaaaggcca taatgcagaa ctgaaccaag tatctaaggc
     1501 atcttcatct tgtttaaaat gagtgcttcc acatttagga cacttatctg gcatttttct
     1561 agcaactact atttctccac actcttgaca ataataagct ggtatttgat gaccccacca
     1621 taattgtctt gatatacacc aatctcttat attttctagc cattgtaaat aagttttatc
     1681 aaatttatct ggaacaaatt ctaattctcc ttttttaaga atatctatag ctggttttgc
     1741 aagttcttcc atcttaacaa accattgctc tgataatcta ggctcaacta ctgtatggca
     1801 tctataacaa gttcctacat tatgattatg cgcttttatc ttaattaggt aaccttgttc
     1861 ttttaagtct gctacgatag cttttctaca ctcaaatcta tccatacctt catattttcc
     1921 acaaacttca ttcatagttc catcttcatt catagtattt aactgctcaa gattatgtct
     1981 taatcctact tcaaaatcat tagggtcatg tgctggagtc atcttaactg caccagttcc
     2041 aaactctaaa tcaacataac tatctgcaac tattggaagt tctcttccta ctaatggaag
     2101 tattgctgtt tttcctatta aatgtttata tctatcatct tctggattta ctgctatacc
     2161 agtatctcct atcattgtct caggtctagt tgtagctatt tctaaaaagt cttcactatc
     2221 ttttagtggg tactttatat gataaaagtt tccgtcgtgt tcttcatgtt caacttctgc
     2281 atctgataaa gtagttttgc aatctgggca ccagtttatt attctatttc ctctgtatat
     2341 gtgacctttt tcatataggc ttacaaaaaa ttctataact gcttcattac acccttcatc
     2401 catagtaaat ctttctttat cccaatcaca agaatcacct agttgtttaa gctgattaga
     2461 tatttttcct ccaaattcat ctttccattc ccaggctctc tttaaaaatt cttctctacc
     2521 tatttcatac tttgtcttgc cttcttgttt ttttattctt tcaacaactt taacttctgt
     2581 tgctatagaa gcatggtcag ttcctggaag ccaaaaagct tcatatccat ccatacgttt
     2641 ccatctgatt aatatatctt gaagtgtatg gtcaagagcg tgtcccatat gcaattgccc
     2701 tgttatattt ggtggtggca tcattattgt aaatggtttt ttatctggat ttggtttaga
     2761 tttaaaatac ccttcatcca tccatttttt atatagccta gcctcaaaat cttttgggtt
     2821 ataagttttt gataaattcg tattttccat tgtcctgcct cctgtatttt ataaataaat
     2881 tttttaaata tctttagaaa taaaaaagag ctcttcatcc tctttaaaaa ggacgaaaag
     2941 ctcgcgttac caccttaatt ccaaagctta aaatacatta acatataatt aaattattag
     3001 ttaatgttat tttatcaact ttgactctca aagaatttaa cggttctaac cgtctaaact
     3061 tacttatagt tcagtttaga aactccaaag ctacattcag taaatcttat acttagaaaa
     3121 tcttacagcc agtgaatttt cctctctttt agtagtattt acttactcct cttcttctca
     3181 gtctttaata ttacatatta ttatatacaa atattattct ctttgtcaat tatttttatt
     3241 ttattttttc tgaattttct taattatgtc tattctcttt tatatagttg ttatatttta
     3301 aaatatgttt ttacagttat tctgtcttat aatgtcaata attgcaagat tattttattt
     3361 taattttaat gctttctcat taattttata ttatattatc cttacagtat acctcttatt
     3421 cgtatttttt atattatatg ttttatttta taaataattt actttatctt ttactatatt
     3481 taaatttatt ataagttttc ttgccagaaa tttttaggtg ttatattttc tgtaattggc
     3541 aatattgtat atcctctctc ttttaagatt cttataatat tatttaaagc atttaaactt
     3601 tgctctttct catgaattaa tactactaat tcatctcttt ttcttccata atataatata
     3661 ttacttacta tctgacttgt agaagacttc caatcctgtg tatccaaatt ccagtcccaa
     3721 attaaataat catttgccat aagtttatta tatgaacctt ctggcatata tggtttactt
     3781 ccgtatggta atcttattaa ttttgaactc tgaccagtta tttcttgtag agtattattg
     3841 catgtattaa attccccaac tgttacttct gggcttttat ataacttatg tatatcatga
     3901 cttacactat gaaaacctat tgagttttcc tcttccaaga ctctccttac ttcttccttg
     3961 tgatttttca tattttgatt aagcataaaa aatgtcgctt tgacatcatt ttcttttaaa
     4021 atatcaagta gtgcatttgt atattttgat ggaccatcat ctattgttat gtatgctact
     4081 ttttcagaag tagcccctgg accttttgtt tcttcttgag atgctttgta tatgatttct
     4141 cctaaagtat tactttgaga aagcagttcc agtgtatttt caccttttga acttatattt
     4201 tcaactggaa cattattaat tttttcctcc aataatgttc cagttaaaat taaaacagct
     4261 ataagaaata tccttataaa tacatgaaaa acagccttat tatctttaat tttcatcccc
     4321 catatctccc ttttataaaa gcataagttt aagacttaaa aagttatttt ttatatttat
     4381 tctctttctt attacttttt atatctctac taaatatttt tttaaccttt tcttcttctt
     4441 tttctcctaa agattctact cttttgattc ccaaagaagt taaaaattgt aatacattta
     4501 aattatcaaa tggagtttcc atattcgtac tttcactata ttcatcaaca ttcttagtac
     4561 cttcttccac acttaaaata cgcttcggac ctcctacaca gcctcctaca caccccattc
     4621 cttctataaa tgtagcttct atttctttat ttagaacttt ctttagaccg tccatgcact
     4681 cttttgttcc ttcaaatgcc ttttcttcaa attttatatt tttgtcaatt cttctgactg
     4741 ataacttaac tgctttactt acacctcctg ttctagcata aactctacct gaaagggacg
     4801 cctctactct attactctca tccattattg ctggatttaa ctctaatgca tcaaatattt
     4861 cttgcaactc tttaaatgta agcacaaaat caactgcatc ttttatatct tctaacattg
     4921 cttctttctt ttttgccata caaggaccta tgaatacaac tttagcatct ggatttaaaa
     4981 tttttacagc tctcccacaa gcaatcatag gagaaacgga tggtgataca ttctccaata
     5041 tctgaggaaa actattttgt atcaaactaa cccaaatagg acagcaacaa ctagttataa
     5101 agcatccttc ttcgtccttt ttcatatgct cataataatc atatgcttct tttgcagtaa
     5161 gcatatctgc tgccaaagca acttctatca tatcttcaaa tcctatcttt tttaatgcag
     5221 ttcttaattt accagggctt acatctttcc caaactgtcc tacaaaagct ggtgcaacta
     5281 ttgcatatac agggctcttt atatctttta acaattttat cattggtata aattctattt
     5341 tatctgaaat tgcacccaac ttacatcttg atacacaatc accacaagca ctacatacat
     5401 cttcctcatt acaattaata tgcttacttc ttatcttaca tgactcatct tccttattat
     5461 tttcatcaca atatttgcaa gcatctgata ttacagatat tattggtctt ttgacttcat
     5521 ctaaatttac agcttcatgt agtgactctt ttaaattttt atctgtatct tctgtcacat
     5581 ccaatcccat agttacccta attaaattta ccatagtatc tttatcaaat tttccactaa
     5641 aataattatt taaattatcc tttaaatctt catttaaaat tttattaggt aagttttcaa
     5701 tatcctctat atcatcattc cacactgctt ttacaagttc gccaaaaagc ttcattttat
     5761 atatttctag tattttagaa ttgtcatttc tagtattcat aaaaaatagt acctccataa
     5821 attaaactta tatatgttta atatttacaa agatactatt ttttatttat tatttaacta
     5881 ctattattaa tatgatttat ttagctttct caacaactct atttcaatca ttattttcat
     5941 tagatgatag ttgaatacgg ggcttttttc ttcatctata ttccagtcta gcttatccac
     6001 attatatgtt ttcttaaggt atttatatgt atgctctttc aaataaagtt tattttcaag
     6061 taactctata gactgcatat gaaaatgtat ttctctacat atccttaagg cattctcaat
     6121 tttttctata ttagtttcac tcaaactata atccaactca tcaataagtt ttgaatatat
     6181 ttcttctaat ttttctactt cttcttcaaa tttctctaca tttaaatttt cttttctaag
     6241 aattttatct tcaataagta acattgaaag ttcttctatc tttttaaatt catctataac
     6301 actatttaga taatttggtc tagccacata ataattaaca agtactccaa acaaaacacc
     6361 tacagatgta tctaacactc tatgtaaaga ataatacaca gggtcactat ttcctacacc
     6421 tagatgtata gccagaaagg ttacacaggc aactactact gactttattt taaacagatt
     6481 gcataaatat attgagcaca ttactcctag accacataaa aaagggtctc cagctttaat
     6541 gatggcaaat atgaatccta ttataccacc taaaatagta ccttccactc tattgattcc
     6601 tgtctttaaa gaacctttta ccgtatcttg aacagaaatt atacatgcta ctagagaata
     6661 aaacatattc tctactagat agttaccaag aatcatacat aaagtgacac taattcctgt
     6721 ttttatagtc ctcataccca tcttttgaag tttcaaagtt ccacctccta aaaagcacct
     6781 tatataaaat acgcatcctc atatttaata tgttcctagt atatcacaat tttttaaatt
     6841 tacccattaa tcatattttt ttatcaattc tttcttaatt tagacaataa aaaaactgct
     6901 ttaaagcagt tttttatcct atctcaacca ttttaccctc aattgcaaaa attatcttct
     6961 cacatacatt tgttatatgg tctccaattc tttcaaggta tcttcctata agtagaagtt
     7021 ttactccttg gtctatagta tttgaatttt catgcattat ttttaaacaa tcaatttgta
     7081 cttgttcata caatccatct acaatatcat ctttagttcc aacatcataa gctatacttg
     7141 catcaaataa gattaaagct tctctagtct gagacatcat agacttacat tcttttgcca
     7201 tcttaggaat atctatcagc tcttttatat aagcctctcc acctatcttc aacatttctc
     7261 tagctatatt tgctgcataa tctcctattc tttccaactc tatcgatata ttacctaatg
     7321 aataaataaa tcttaagtcc cttgccattg gttgctttaa tactattagc tctatactct
     7381 tatctcttat ttcttctctt aatttattta tttcttcatc atcttctact acttttcttg
     7441 caccctctaa atccttctct atcatacaag tcacagactt ttctattgct tcttcacatt
     7501 tttccatcat aagtactgtt tgttctttta aagtatcaat acttatatct aaactattat
     7561 tgaccattgt ctaactcctt tatagttata tattttaacc aaatctacct gttatatagt
     7621 cttctgttct tttatctaca ggagtggtaa acatagtttt tgtatcgcta aattctataa
     7681 cctctccatt taagaaaaaa gctgtttcat ctgatatacg agcagcttgt tgcatattat
     7741 gtgtaactat gactattgtg tagtcttttt ttaaatcctc tataagttcc tctactttta
     7801 atgttgaaat tgggtctaaa gcagatgtag gttcatccat aagaataact tctggtctca
     7861 tagcaattgc tcttgctata catatacgct gttgttgacc accagaaagt cctagtgcag
     7921 atgatttaag cctgtcttta acctcatccc aaatagctgc accttttaag ctttcttcta
     7981 caattttatc taattgcttc ttatctctta gtccatgtgt acgaggacca tatgccacat
     8041 tatcatatat actcatagga aatggatttg gtttttgaaa taccattcct actttagtac
     8101 gaagatttat aacatcatct gaggtatata tgtcctctcc atcaacagaa atgtttcctt
     8161 taattgttac atcttcgatt aagtcattca ttctatttaa cgttcttata aaagttgatt
     8221 ttccgcatcc agatggacct attaaagcag taaccttatt ctcttttata tccatattta
     8281 ttttctttaa tgcttgctta tcaccataaa ataaatccaa atcttttaca ctcattttta
     8341 tcttatcgat taattccatt attcacacct cttaaattta ataattagct ttatttaatt
     8401 tcttagcaac aaatcttgct agcatgttta gtgctaatac aattactatt aaaacaattc
     8461 ctatactggc tgctgttgct atatctccac tctctttagt cagtaagtat gagtgtacag
     8521 ttaatgttct tgcactatca aatattccac ctggcatctt ggctacagta cctgctgtaa
     8581 gtaatattgc agctgattct ccaattatac gaccaactga taagattact ccagataaga
     8641 ttcctggtat tgcacttgga agtactacct tatataaagt ttgaaatttt gtagctccta
     8701 atgccaatga tgattctcta taagcttgag gaactgtttt taaagcttct tctgttgtac
     8761 gaattattac tggtaatatt attattgcaa ctgtaagtga acctgcaact atggaatatc
     8821 ccatttttaa tgtaactaca aagaaaattc ctccaaataa tccatatact atagatggta
     8881 tccctgataa actttcagtt gcaaatctta tagctgtaac tgcttttcct tttttagcat
     8941 attcttgtaa atatatagca gccaatattc ctataggagt tgctacaact atagaaacta
     9001 ctactgtata taatgttgta attatcattg gaagtatacc accatcacct gatgctgaat
     9061 aattgctaaa taagaaatta aaattcatgt tgccaattcc ctttataaat ataaatccca
     9121 ctataatgac taatgatact attgtaaata gtgctgatag atatactaat gacttcaata
     9181 gattctcttt gaactttctc atcttaattt accgccttat ttgaaagttt atttaaaact
     9241 aagtttaaaa ttaatataaa tgtaaacaat actactccag ttgcaaataa catttcttga
     9301 tgtgtaccaa atgcgtatcc catttcaagt gctatattag ttgtaagtgg tcttacacta
     9361 tctgttattg cagttggcat aactggtgag tttcctgcaa caagtattac tgccattgtt
     9421 tcacctaaag ctcttcctac tcctaatact actgcagcta atactccaga tttcgcagca
     9481 ggtagtacaa ctttgaatat tgtttctatt tttgatgcac caagtgctaa tgaaccttct
     9541 ttgtatgctt ttggtactgc tcttattgct gtttctgata cagatattat tgttggtaac
     9601 atcattactg ctaatactat tactattgct aacaaacttt gaccttttgg aagattaaat
     9661 atactttgta tagttggaac tatcactgca agaccaaata caccatataa tactgaaggt
     9721 atacctgcta aaagttcaac tgctggtgac attatttttg ctaatctttt aggtgctact
     9781 tctgctataa atacagaggt taatatacca acaggtactc ctatcacaag tgctccaagt
     9841 gttgcaaata ttgatgctac tatcatagat gatattccaa atttattggc agaaggaacc
     9901 caatctgcac cagttaaaaa gtctataaat gaatatcctt caaatataaa tggtcttaag
     9961 cctttataaa atacgaatcc tattatcaaa agtaaacttg caactgctac taaagcacta
    10021 agtagaaaaa tattttttgc tactttttca attacgtatt ttgtcttatt gccattattg
    10081 tcattttttt ctaaacctac taatctagat tttactatca taataccctc ccattaaaat
    10141 atacttatcg attttcgttc tgtattcaaa atttaatttt ttataattcg tcttcatcaa
    10201 atttcctgta cttttattct acttttttta agttaaatct atattaggat taagttaagc
    10261 aagtgtaaaa ttatatttac ttttttaaca ttaattttac ttttaaaact atatttgaca
    10321 tttactttac aatatactgt tgtttctcta tattaaaata gcagatatag ataataatac
    10381 agtttatgta taatttatta cctatatctg ctattaagat ttgttaacta aatttaactt
    10441 ttatactaca tatataatta tcgtatcctc tagtttatat cactattatt tctatcaaat
    10501 tgttatgata cttgatgttt gcaataggta cgaatagcca aaaatataaa aataggtatt
    10561 tctatcactg agaaaaacag tataatgtgt ggtgtccaaa aactcatacc tcctaagtgt
    10621 aagaaattaa atgatgtcat ttgatataat aaactaatct taagaccaat tccccctggt
    10681 ggtaataggt atccaatcca actaacacct attcccatag gaattaagca aatagaaaaa
    10741 gcaatcagca tagaagtaag agaatcctta cattttgcag ataaaaataa tgttaaacta
    10801 accattgcaa gtattgaaat tagaccacca atagctataa gaatctgtgc ttgtagtaaa
    10861 tttatattta ctagactaat gacagaaaac aacatttgaa atgaggtttt caaacattca
    10921 gtaccaaaag ctaaatcaga aattgccaaa tggatagaaa tacatacaac aaacattact
    10981 ataaatatag aaaatagagc taatatttta gtaaacgcta gacgagtatg tccatgtttc
    11041 gtacaacgaa ggatgctatc tgattgtgtc tgatactcac cagaaaaagt aggtgcagta
    11101 attgcaacac atataattaa taatataaaa atgtaaaata ttatataatc gaaagcatct
    11161 ttagaataac ctgggtatat ctcaaatgga gtatctactt ttctataaag ttcttgagct
    11221 ttcatttgtg cactggaagt ttgtcgttca atcttcataa tatcatttaa atgagttttg
    11281 cattgttcat aaaaagaatc taaatcattt gtatttattt ctgataattc tgttgcaaat
    11341 cctgtttctg aattagcaaa tgcttcagga agtctattca acaatgggcg tattggtaaa
    11401 atacttttag aataaatatc tagtgggaaa tcttcactac ctactttacc atacttttct
    11461 tctgtttttt tccatatatt taaagcatgc tttaattttt ttggtgttac aactccacta
    11521 ctaatttcac tattattagc ataaattgtt tttttatatt taatagcatc taaaccactc
    11581 aattcaacta ccttgccatt attatctaaa taattgatac cctcgtaagt aattggaaga
    11641 aaagccatca atgcagataa aaccaatgcc aacaataata aaattaatgt acgacgtgag
    11701 tgcaacactc gtttaatttc caaataaaaa atacgcattt atttcccctc cttactataa
    11761 actgcttctg gaaataacca taaatataaa tcttctagtc gtggttcaac agcaatagag
    11821 ttatcaattt gtgacttttc tgccaagtaa cgaatagata cttgattgtt tttttcacta
    11881 cgctgattga ttatacgtaa attcatttca tattttgaaa tatctctatt tgatataaga
    11941 cattcccata ctttgccctc tactttctta accagtttat cggttgttcc cacatcaaga
    12001 atttttccac ttttcataat agcattttga gttgatatgt actcaatatc agatacaatg
    12061 tgagtagata ttaggacaac acgattatga gaaaattctg aaataaaatt acgaaatctc
    12121 actcgctcac cagggtcaag ccctgctgtt ggttcatcca ttataaggat tttagggtca
    12181 tttaacatgg cttgtgcaat tcccactcgt cgtttcattc caccagataa tttaataatc
    12241 tttttatttt ttacatcaga taatgtaaga atttccaata gattattaat ttttcctttt
    12301 acttctcttg gtggtacatc tttcaatgct gctatatatt ctaaataatc ttttacagaa
    12361 aaatcctgtg ggaaaccaaa ttcttgtggt aaaaatccca gtaaatttct gtactcagca
    12421 cccattatat aaatattttt tccattatac agaattttgc cattggtagg tttcataatg
    12481 tctgtaataa ttctcatcaa ggtagtttta cctgctccgt ttgctcctag aaatccccac
    12541 acacctggtg ttaaggtaag gcaaacatct tctacagcaa ttttatcctt aaattttttt
    12601 gtcacatgtt caatttttaa ttccatattt gaaactcctc tctttatcta cttacctgca
    12661 aaaacaaaag tgtcttgctt agtatttatt ttactaaaca aagacactca taattttaat
    12721 attaaataga taatttctta agaaaatcct aatatttgat ttatatttta ctaaatagat
    12781 gtaggaagcg atatagtaaa tgttgtgact tcatctttac tttgaacagt aatatcacca
    12841 ccatgcaaat ttataatttc cttagctata gaaagcccta aacctgcacc acccatatca
    12901 gattttctag cttcatctaa acgattgaac ttttcaaata tagcagaaag ttgttcttct
    12961 ggaattgtct gtccataatt tctaaaagca atatctacct tgctgtcttt atgaaccgct
    13021 gagataataa tttctgtatt aggatagctg taagcaatag catttttcaa gatattatta
    13081 aacactcttg caagtttttc agaatctgct tttattgtta aactttcatt tgcttttaga
    13141 atggctctat ttcctttttc agacagcaat gggtaaaatt catcctttaa ttgaagtaac
    13201 atataatata ggtctacttt ttcgaaatta attttgattt gattagaatt atatcgtgtt
    13261 atttcaaaaa gttcattgat aagtttttct aaatattgtg ctttgtcaag tgcaacttca
    13321 gtatacttag atttttgaga attatccata ttaggtattt cttttaataa gctcaaatac
    13381 ccaataactg aagtaagtgg tgtccttata tcatgtgcta gatacatcac aaggttattc
    13441 tttttatttt cagcctcttt cactgcttgt tgacttaaca aaacagacat cttaatttgg
    13501 ttcatttgtt gctctatttc ctttaatggc ttagatagtt caaccaattc atcattttgt
    13561 tgataaatag tatgtgttgc attgatgatt tctcttaaat aattccatgg ttttttccaa
    13621 taaaaattga aaatagtgat atatccaatt ataaaaacca aaaaaaataa tacatctctt
    13681 ctcaacttaa gccatttaac aagaggattt gaaaaatttg aaaacgtata atcagccata
    13741 aacattaaaa ccaatactat acctgtatag atagctaaca ttatatataa tttatacttt
    13801 aaatacgcct ctgtattttt ttctcttttt ataaatttat atttatctct tagtttccta
    13861 taagtaaaat tattaatctt tttcataata ctacatccct tcttgacaag aaaaataggt
    13921 gtcaaatctt gtaaccaaca ccccaaattg tttttatata ttttgcttca tctacagtat
    13981 cacctaattt ttcacgaaga tggcgaatat gaactgttat agtattgtta cttttattgt
    14041 aatattcatc tttccagata gcatgaaaaa gattttctga atttaccaca gagcctttat
    14101 ttttcaataa tacatgtaga atagaaaact ctgttggagt aagtgaaaga gctttaccat
    14161 ttaaatggca ttcatatgtt tgggtattca attccagacc taaaaaagaa aaaatatgtg
    14221 aaagttcttc accagattgt aaacttccac tgtatttctt atatcttcta atctgtgcct
    14281 tcactctagc aacaagttcc aaaggtcgga aaggtttagt aacataatca tctgcaccta
    14341 aagttaagcc tgtaatcttg tctgtttctt catccttagc tgttaacata atgattggat
    14401 aagtatattt ttctcggatt ttttggcata aagaaaatcc atttactcct ggcaacataa
    14461 catctaaaat tgcaaggtca aattcattta aattaatgca gtttagtgca tcaagaccag
    14521 aataaaattt ataaacaata tagttttcat ttgtaaggta gacttctatt aaatcagcta
    14581 tatcacgttc gtcatctaca actaaaattt ttccactcat agactttaaa catcctttct
    14641 atatatattt atatatatta tactaaattt aattttttta tatgattttt tacattagaa
    14701 tttattaaga aattcttaat cttattatgt aactatttta ttatttaaca tatattaaag
    14761 ataaaaatga atatatatag atttagatat acaatttatc tatcttttca aatataatct
    14821 ataaaataat agttctgtta cgcatttttt catataataa aactgactgt aatttaactg
    14881 gcaaagtcta gaagaatagg ctttatcaat taaattacag tcagctattt tataataatt
    14941 tagtttagat atcgttagca tttcacattt ataacaatta ttaatgagat taatatattt
    15001 attattcttt attaaaataa tatcctgctc ctcttattgt tataattttc tgaggatttt
    15061 tataatcttt ctctattttc tttcttaatc cattcactgc aactaccaca ctatagttat
    15121 cctctaagta ctcattcatc catacatgtt catatatctc tttagtagtc agagtctttt
    15181 caggattctc tataaaaaag tttaacagct tattttctgt tggagtcaat tttattattt
    15241 tgtcttctaa ctttattact ccactagttc tattatattc ctctaatttt gtattatttt
    15301 cattattatt tacatatgag ttcattcttc ttaaaatagc ttttactttt gcatataata
    15361 tttctaaatt tagaggcttt attaagtaat catctccgcc agaatttaaa gcatttaaaa
    15421 tgctttgttc ttcatttata taagtcatat aaacaattgg acaagtagtt acatttctaa
    15481 tttttttaca aagagtccat ccatcaccat cagttagaat tatttctaaa aatattaaat
    15541 catatttatt gctaaatatt tttccaatag cttcttcaca attatatgct aaatctatac
    15601 ttatttcttt ttcctccaga aatcctttta gcttctggca attatccttg tctccatcta
    15661 ttatcaatat cttagtttgc attataatca ctcccaaata tcattattct tttatattaa
    15721 ataagctatt attataaaaa acatagactt aattttattg taaaaacagt tccttttcct
    15781 agttctgaat ctacaaagat ttctccgcca tgctctctta taatggattt gacaatatat
    15841 aaccctagcc cttgcccttt tggctcatta ttatctcttt ctgtaaagaa tctgtcaaat
    15901 acctttggta tattttctgg aggtatacct tttccattat cagaaacttt aataattgca
    15961 aatccatctt ctttatccat tgataaaatt atctttccat caaatggagt aaatgtcaaa
    16021 gcattataaa ttaaattctc aaatgctctt gtaagcatct ctttatttat ctttgcatat
    16081 atttttttct ccaatatgta agtttcaaag tttacaccac atgcttgtgt atctggcaag
    16141 ttattttcat atatctcttt aataaaatca tttagacata ttttttcact aataccaatg
    16201 cttttatctt gtgatgtaaa ttcttgcaaa ctacgtactc tattttgtac ttcatttgac
    16261 ttttgattta tagcataaag ataggttctt gtctcttcat ctacatgaat attaccaacc
    16321 tttatcaagt ctataaatga ttgtatagct gaaacaggtg cctttaaatc atgtgccaca
    16381 tctgccaaga attttttacg ttcatttaac aatgttgtaa gttcattagt tctcacttca
    16441 acttcatgtt caagattcct tgtaagtttt tcattctctt ttattattat tctattcctg
    16501 tttatcatca taagagaaaa taaaataaca attataaatc cacaatattc tgtttgccac
    16561 cctgtaaata taggctcaaa tctattacta gtaactgcat caaataatat gctaattcca
    16621 aaaacagtgt ttactgaaac taaaatataa gaacttatat gctcagagca tgtcttttta
    16681 tatattgaaa ccagcgaaga cattaatata tatataaata caatatattt atatatgtct
    16741 attaatttcc catatatgtt gacaaagtac atatcatatg gaagaagaat aattggtatc
    16801 actatagata tgatactcat tgtaattcct aatggtaaaa ttaaaaatct atacacttta
    16861 tcttcttcta ttttggatat acttccattt atacaaataa tacataaaac taccataaaa
    16921 taggacgaat cttcaaccgc atatgtaaag cttaccaaag gcagtcctaa caaatgtaca
    16981 aagggatagg atacatggat ggcaaagaaa acacacatac atccaaaata taagtaaagt
    17041 ttacttttgt ttgaaagaaa ccatacagac aacgaaaata tagctattgc aaaagttgtg
    17101 aagcaaatta tactataaaa aagtaatcga taaaacatca tattacttat gtcctttgtt
    17161 tctcctaatg caggcggata atacaaacca ctataataat gtgaaaaatt cgatacattg
    17221 attataattt ctgtatcttt ctttgcaata aaacttacta ctgaattttt aattttgggt
    17281 ctatattttt ctgttcctac atctcctaat ttagaaacta tttctccatt tatccatact
    17341 tcacttgcgg aaaaaatctc aggaagctct aaacttagta gcttatcctc accttcattt
    17401 tttattctca tacgataagt agcattccca tatggtgact gattcccttt tctcatagaa
    17461 aaatctggat attgacctat aaaaacatat tctggtttta tatcttgctc tgtaaaatca
    17521 ttcggtttaa atattttatg attataaaac tcccagtcat ctatcagaaa gacaagttta
    17581 cctttagtga aagtatcatt atctatagtt aaaattccat tacttgctct aatagactta
    17641 tatgtatatt tattatcata tttatacaga agatttaatc ctgctattgc caacagagag
    17701 actatcaata caatgagagt tccttttagt atattatact ttttcattgc atcaaccaca
    17761 tctctttcat agtagtatta aataaaacaa gaactattta ttaattacag tatctcactt
    17821 atggtacaaa cttatatcct tttccccata cggtttgaat gtattgatgc tccctgctta
    17881 aagaattaat tttttttctc atctgtgcaa tatgaacctg cactgtatgt tgatctccaa
    17941 tatcaggcat tttccaaact tggttatata tctctatctt cgaaaatact tgttctttgt
    18001 tttcagctag aaacaataat atttcaaatt ccatagtagt taaatctata ctttctccat
    18061 caaaagttac ttttcttgaa tttacattta ttactaacgc tgaaaacttt aacacattag
    18121 gaactgatgt aatatttttg tactgcctca tacgagcata tattctaagt tcaagttctt
    18181 ttaaactata aggttttgtt atataatcat cccccccagc taaaaatccc cgaacttttt
    18241 cttcttcatg ctcataatta gataaaaata ttataggtat gttaaccttt tcttttatat
    18301 cattgcacat tttatatcca tctatatctg gcaatgaaat atccaaaatt atacagtcta
    18361 aaactatacg ctctactata tccatagcat ctttagcagt ctctgatgta tatacattaa
    18421 aacctatatt agaaaaataa atttgattta tatttaaaat atcaacatca tcatctacaa
    18481 ccagtaaatt atacattaca ccactccatt caaaggtatt ttaatcataa tctcaagttt
    18541 tataattatt acaaattata atgttttatt ttcatatata actagtatta ttattttata
    18601 atatctttac aaactagact at
//
LOCUS       DACTGW010000064        18060 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.064, whole genome shotgun sequence.
ACCESSION   DACTGW010000064 DACTGW010000000
VERSION     DACTGW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18060
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.064"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(77..1174)
                     /locus_tag="I9573_001687"
     CDS             complement(77..1174)
                     /locus_tag="I9573_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HAT4796313.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILFTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            1467..2993
                     /gene="glpK"
                     /locus_tag="I9573_001688"
     CDS             1467..2993
                     /gene="glpK"
                     /locus_tag="I9573_001688"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAT4796314.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFESGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKEIKMLRVDGGITKNNFTMQFQSDILDIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTKQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            3034..4149
                     /locus_tag="I9573_001689"
     CDS             3034..4149
                     /locus_tag="I9573_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HAT4796315.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(4430..5194)
                     /locus_tag="I9573_001690"
     CDS             complement(4430..5194)
                     /locus_tag="I9573_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAT4796316.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            5453..5543
                     /locus_tag="I9573_001691"
     tRNA            5453..5543
                     /locus_tag="I9573_001691"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5487..5489,aa:Ser,seq:gct)
     gene            5631..6419
                     /locus_tag="I9573_001692"
     CDS             5631..6419
                     /locus_tag="I9573_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HAT4796317.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHIGVLFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSVDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            6440..7180
                     /locus_tag="I9573_001693"
     CDS             6440..7180
                     /locus_tag="I9573_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAT4796318.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDSLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAVVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            7358..7732
                     /locus_tag="I9573_001694"
     CDS             7358..7732
                     /locus_tag="I9573_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796319.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKDNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            7824..9284
                     /locus_tag="I9573_001695"
     CDS             7824..9284
                     /locus_tag="I9573_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAT4796320.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTIGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIEEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLNVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFETWFESFKAELENAIKK"
     gene            9337..12213
                     /locus_tag="I9573_001696"
     CDS             9337..12213
                     /locus_tag="I9573_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT4796321.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKILNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILNDKNINDKPIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRIEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKDKKLKWIRMNIFEDELKVLG
                     VVVDVTKEFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLD
                     NLKYINDTYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRD
                     IINSVKKQMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTI
                     KGEVSEFDINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPK
                     IKTFSSPIDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLS
                     KVDFEEIEDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNG
                     DAVLLYIMPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVISEGVETKGEME
                     TLINLGVDYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            12290..14356
                     /locus_tag="I9573_001697"
     CDS             12290..14356
                     /locus_tag="I9573_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAT4796322.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVYTANRLIDKNKNRYEKVIKCSQGDGGSVTYISPHDSEEEAL
                     YIAKEIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            15384..16199
                     /locus_tag="I9573_001698"
     CDS             15384..16199
                     /locus_tag="I9573_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAT4796323.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            16227..16904
                     /locus_tag="I9573_001699"
     CDS             16227..16904
                     /locus_tag="I9573_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAT4796324.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            16963..17685
                     /locus_tag="I9573_001700"
     CDS             16963..17685
                     /locus_tag="I9573_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAT4796325.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         7083 a         1916 c         3222 g         5839 t
ORIGIN      
        1 tttattatag gaaaatttaa aaaaatcagt ctatttaatt tatactgatg tattgtagga
       61 ttttacaaac aaactgttat ataacttttt ttatctccaa ttttgatttt aagatatcta
      121 agaatttata acaatcttct ggatatattt ctcccatatt tgcaacttgg aatatgttag
      181 catctaggaa tttgcctttt cctggataga caacatagcc actttccctt tctaggccta
      241 atataaactc atttacattg atttctttag gtagaaatac agatgttact gtattagaca
      301 tatacttatc tggaagtaaa aatgttagcc ccaattcttc catgccattc cttaaaatag
      361 cagaacattc tttgtatctt tctattttac tgtcatcttc caaaaactca gttaaagctt
      421 cattaagggc aaatattaaa gtaacattag gtgtatttgg tgtttgagaa gatgatttag
      481 caactttata gtgtttataa agattaagat atacattctt acattgattt tctttaatat
      541 tttgtaaaat actctcttta gcacaaaggt atgcagaacc tggaaatgct ccaagagctt
      601 taccaccaac acctgttatt atatctatat taaactcttc tacatcaata tattcacctg
      661 ctgaggcact tattgcatct acaaaaaata ttttgttata cttcttagca agttttccta
      721 gttcagggac tggatttatc attcctgaac tagtttcatg gaacaccata gcaattactt
      781 ttatatcaga attttcaatt atatattttt ctaaaatgtc caggtctggg tattctgccc
      841 attcaaaatt aggcttatat gtttttacat tataagtaaa taatatttct tctagccttt
      901 ctccaaaaac accattacta actagcagaa ctgcatcttc tttgtcaaag atagaagata
      961 gtacacattc atttacagat gttccagagc cagatacaat taatgagtag taattagaag
     1021 ttgcattata tagtttattg attttttgtt gtgtatcttt gaataaatcc ataaactcgt
     1081 ttcctctatg gcatatatca tagtgaagaa gtgaatttct gactttatct gtaaccatta
     1141 ctggtccagg actgaataga tatgtttttt tcattgtaaa gcctccttaa aaagttaaat
     1201 ttaaattttc gaatgactga ctatgtgtaa atattaaaat attgtgtgtt attttaacta
     1261 gattatagaa taaaataatg aatattgcaa taatatttca ataaaaatac ttttttaatc
     1321 accataatta aaaataacaa aaaatattgt aatgaatatt gcataaatcc acatgatgat
     1381 ataaaatata aataacttga atagattata tattcaaaaa ataaaaaaga atttattaat
     1441 tctgaaaata aagggggaca ttttaagtga aatatgtatt agcaattgac caaggaacaa
     1501 caggaacaag agcagtattg ataaacgaaa aatcagagtt tgtttttagc gattatatgg
     1561 aacatactca aatatatcca caatctggat atgttgaaca tgaccctata gaaatttgga
     1621 ataatacaaa aactgtgagt aataatgtat tacaacaggc ttttgaaagt ggaatcaatg
     1681 aaagtgatat aaaaggaatt ggtatagata atcaaggtga aacagtaatg ttatgggata
     1741 aaaatacagg taaaccactt tacaatgcta tcgtatggca gtgtaggaga acttatgatt
     1801 atgtggaaaa tttaaagaat ataccaggtt tagaaaataa gataatagat aagactgggt
     1861 taattataga cccatatttc tcaggtacaa aaattaaatg ggtaatagat aatgtaaaag
     1921 gtgtaaaaga aaaaattaaa aatggagaag tacttgcagg aacattggat agttggttaa
     1981 tatggaaaat gacaggagga aaatcttttt taactgatgt atctacagca gcaagaacaa
     2041 tgctttttaa tataagagat atggattggg atagagagtt gcttgacatt ataggagtgc
     2101 ctagaagtat tttagcagat ataatgccta ctagtggtga ttttggaact acagataagg
     2161 aggcattttg tggggtttct ataaaaatta ctgcttcaat ggtagaccaa ccagcagctt
     2221 tatttggcaa tggatgtttt gaaccaggaa tggttaaaaa tacttatggt acaggatgtt
     2281 tcttatatat gaatataggt gaggagttaa atatagcagg taatggtatt ttgacaacag
     2341 ttgcttggaa gataggagat aaagttacat atgcttatga tggtggagtg tatatagcag
     2401 gtgctgcaat acaatggctt agagatggaa ttggagttat aaaaaattat tcagaaactg
     2461 atgacatggc aaattccata tcaagtactg gaggggtata ttttgtacca gcatttgcag
     2521 gtatagcagc accttattgg gaccaatatg ctagaggcac aatggttggg ataacaggaa
     2581 gtactaaaaa agaacatata gtaagggcta ctcttgagtc tgttgcactt caagtcaagg
     2641 atgttgttga ttccatgaat ttggcttcag ggaaagaaat aaaaatgttg agagtagatg
     2701 gtggaattac taaaaataac tttacaatgc aatttcaatc tgacatatta gatataccaa
     2761 ttgaaattgc agaaaatcct gaaacgacag tacttggtgt agggtatatg gcagggcttt
     2821 cctgtggtgt ttggaacagt attgatgaaa ttagaagtga atttacaata agcaaaaaat
     2881 atattccaaa tatgactaag caaaaaagag aagaaatatt acttggatgg aatcaagcag
     2941 taaaaagagc tatgaattgg gaaaacgata ccaaaaaggt gtgtgctaat taagttacaa
     3001 tagagtattt tacatgtata ggaggaatta aaaatgccct ttgataaaat tatattatac
     3061 ataatggctg tgggagtgct tttaggagcg ttagatagaa taattggaaa taaatttgga
     3121 cttggagagc agtttgaaga agggtttaat tcaatgggtc cattggcttt aggaatggtt
     3181 ggtatagttt gtttagctcc agtaatttca gatgtactag gtcctataat aataccaata
     3241 ttcaatctct gtggagctga cccatctatg tttgcaacta ttttagcgaa tgatatggga
     3301 gggtatccat tggctatgga acttgcacac aataaagagg caggaatgct tgcaggatta
     3361 gtcgtgtcat ccatgcttgg atgtacatta gtattttcaa ttccagtagg actaggtctt
     3421 atagaatatg aagacagacc attctttgca aaagggttac tgataggctt gataacaata
     3481 ccttttggag gtattatagg aggattaata gcaggattta acttatcaat ggtaattata
     3541 aatatgatac cagtgataat attggcagta atattggcag taggtttaaa atttaattct
     3601 acacttatga taaatgggtc attagttttt ggaaaattta tatctatatt gataacgata
     3661 ggtttgggag cagcagcatt tcaagaaata acaggtatag tacttataaa aggcatgaca
     3721 cctataagag aaggtattca agtaattgga agtattgcaa tagtgttatt aggtactttt
     3781 ccaatattac atttattagt taaagttctt aataaaccac ttacaatagt gggtggaaaa
     3841 ttaggtatgg atgccactag tgcagcagga ttagttttta ctttagccaa tagtattcca
     3901 gtatataaaa tgatgaaaga tatgagtcca aaggggaaag tagtaaatac tgcttggctg
     3961 gtttgtgcta cagcagcttt gggggaccat ttaggattta cagcaggagt agttccagag
     4021 atgattacac ctgttgtaat tggaaaattg gttggaggag tattggcaat tgcacttgct
     4081 atacttatga caaaagattt aactttggaa gaagaacaaa gtattaaaat gaagaaagat
     4141 gcagtgtaga ataaacaata cttaagtcaa atttaagtta ttttaaagta ggagtttaaa
     4201 taactaaaaa taaattaaaa agacagacaa ttaaaaaata atcgaataac caaaactgat
     4261 aaattaaata aaatggcaat caaataacta aaaatgataa gctaactgaa agtagttaca
     4321 taattaaaat ataaagtata gtttagacat aagtggagct aaataaataa ttatttggct
     4381 ccttaatttt aatttattac atcaaggaac taaaaatcaa tcatacaagc taattatcag
     4441 ctatgactaa aggtattcct ttttgagaaa actctattat tatattatgg tctatactag
     4501 cagaggtaat aatagtatct atgtgttcta taggagctac tgatgcaaat gaatttatac
     4561 caaacttagg aggttcgaca actactatag attttctaga cctttcaatc attttttttc
     4621 taaccagtgc ttcttctaag tgatagtcag agattccatt ttcaagagtg atacctccag
     4681 cacctaaaat agctttatca acattaaatt gttcaatagc tgagagtgta agtggaccag
     4741 aaaaagaacc ttcagtttct ctaagatagc caccaagcac atatactttt atattagtat
     4801 tcataagttc atttgcaact aaaattgagt tggttattac agttagattc tttttatctt
     4861 tcaaattttt tgaaacttca agtacagtag ttccagtatc cataaaaata gtatcatttg
     4921 tatttataaa ctcactacat ttaatagcaa tctgtttttt atcgtttgag tttatttttt
     4981 ttcttttaac atagttaagt tctacgctac ttgctataac tggtatagca ccaccataaa
     5041 ctctttttaa aagaccgata ctttccatat gggctaaatc tcttcgtgca gtttctattg
     5101 atatatcaaa catttgaact atgtcattta ttttaattat attatctgat ttgagaatac
     5161 ttaaaatctt ctcgtatctt tcctgtggta acataaaata tcctccttgg actagtatta
     5221 ttttagtttt ggcataactt acaattaagt cagagtttag gtatatttgt taatgtatgt
     5281 atatttatca aataatatta taagtgttat gcaagataat tttaccatat ttaaaactta
     5341 catttaagca aaaaagtaaa aatagatgtt taataattca aaataactca aattttataa
     5401 aacaattgac taaaatatga ttttattata taatttacta gtatagagat atggagaagt
     5461 actcaagtgg ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg
     5521 ttcgaatccc tccttctccg ccaattgtta aaggctattt ttattaaaga tagtcttttt
     5581 ataatgtcat aaatgattag atatgtataa ttaaagaaag gagggaatag atgacaactt
     5641 taattttttt catagtgggg atagcatctt tggtagttgc ttttgttatt gatgggggac
     5701 atattggagt attatttgta ttttcatcag cacttgttgt gtttggtgga actataggcg
     5761 ctgttggagc ttcgactcca ttccctgtgt ttaaaaagtc tataaagata atgttattag
     5821 cttttaaaaa taaaaagcct aataacatag aaaatataat ctatttcaaa gaaattgcaa
     5881 caaaggctag gaaagagggg cttcttagtg ttgacaaaga aataagtgag aatgatgaat
     5941 tagacccttt tattagaaaa gggcttgaac ttatggtaga tggggtggat gcagttacta
     6001 taagaaatat attggaatca cagatacaga taacatcaaa acgacataaa gaaggaatag
     6061 caatttttga ttcagcaggt ggatatgcac ctacactggg gataataggt acagttatgg
     6121 gattagtaca tattttaggt aatctttcaa gtaatccaga tgcattagga cctcaaattt
     6181 ctgttgcatt tttggctact ttgtatggtg ttggttctgc caatttaata tggttaccaa
     6241 tagcatcaaa acttaaggct ataaatcaac aggaaataag tgaaagactt ttggtgatag
     6301 aagcaatatt atccatactt gagggtgata attctaattt aatgacagaa aaattaaaaa
     6361 tatttttaaa ttcaaaagaa ctaattgaac ttgaagaaat tggtggtaga gctgaataat
     6421 ataaaaggat ggaatgttta tgttgtatga tgaagatgaa aaagaagaaa acaatgagcg
     6481 atggctttta acttattcag acctcataac attacttatg attttctttg taataatgta
     6541 ttctatgagt aatgttgatg cggagaaata taaacaatta tctcaatctc taaattctgc
     6601 ttttggtggt tcttctggag ttattgaagg tggtaatagc aaaatagaac cagtagttga
     6661 accaggaagt aatgatttag atagtttaca aaatgctaag tttaaaaaag ttggtgaaga
     6721 aatccagaag tatttaaatg aaaatggtat ggctaattca gtatcattaa gagttcaaga
     6781 tagaggatta gtaataagcc ttaaagatac aatactattt gatacaggaa aagcagttgt
     6841 aaaggataat tctagagata agattattca aataggtaag atgttaaatg aaatgaatag
     6901 ttacatgaga gtagaaggtc atacagacaa tatgtctata aaaaattcag agtttaagtc
     6961 aaattgggat ttatcagtta tgagagcaac caatgttgtt caacttctta ttgacaatgc
     7021 aggtatagca ccagataaat tatctgctgt aggatatggt gagtttagac caatagcaga
     7081 aaattcatct gaagaaggta ggtcaaaaaa tagaagagtt gatatagttt tagttgatag
     7141 taaatatgat aatgtagaaa atgtttcaaa ggataaatag ttaaattagc tgtctcaata
     7201 cagaattaat tctaaaggag atggcttttt tatgcgaaaa ttaaaaatag atacatagta
     7261 agttaccttt ttgtaggaat atatggtatc ttaaatatat aatatttaaa taatatataa
     7321 gctcaattaa aaaaatttta tagaggatgg gaaataaatg gggaataaaa ataaaaatac
     7381 gaaatataaa gataaaacaa gagaaagata taataaaaaa gttgagaatt taaataaaga
     7441 aaaattaaat aaagataata aacagtctaa gaaggtagat aagattgatg atattattaa
     7501 taaatctagt attgtattat gtgtacttct agtggtgttt tcattacttt acttatttaa
     7561 attggttcaa cttaatataa taatgatact tttagtaatt acagtagcta ttcaacaagg
     7621 attggtgtca tttggtgcat ttagaaataa aaattatgta aaagctttat tttctatatt
     7681 tgtaatagta tgtttcttaa ttgcttttaa atatgttgta aatcctgttt aaatatatca
     7741 ataaacgttc tataattaat ataaatagtg aagttacagt tttaatgcta ttataaattt
     7801 tataatttga aggagtagat tttatgaata ggtgtaagtt tgcaaaatta attatcacat
     7861 caatgattgt tttattactt acaggatgta gtttaaaaaa ggaagataat tataagttag
     7921 acccagaaaa tccgacatca atacaaattt ggcattatta taatggacag caaaaaatgg
     7981 cttttgataa gcttgttaag gaatttaatg acactatagg tgctgaaaaa ggaattattg
     8041 ttgaagcgtt tagtcaaggt aatgtagttg atttatcaga gaaagtttta gattctgcaa
     8101 ataaaaaagt tggctctgaa gatgtgccaa atatatttgc agcatactca gatactgcat
     8161 atgaaatgga taaattaggg ttactagtag atatggataa gtacataagt aaagaagaat
     8221 taaatgaata catagacgaa tatgtagaag aaggcagaat aaattctaag gatgagttta
     8281 aacttttccc aattgcaaaa tcaactgaaa ttttgatgtt aaataaaact gattgggata
     8341 aatttgcaaa agctacaaat tcaaatataa atgatataaa aacaattgaa ggattagtag
     8401 agacatcaaa aaaatattat gaatggacag atagtttaac gaaagagcct aatgatggta
     8461 aagcattttt tgggagagat gctttagcaa attatattat tataggaagt aaacaactag
     8521 gagtagaaat tttttctgtt aaagatggta aagttacact aaatattgaa gaaaaagtta
     8581 tgaaaaagct atgggataat ttctatgtac catatataaa tggatatttt gcttcttatg
     8641 gtaagtttcg ttctgatgat gcaaaaacag gtgatatatt agctcttgta ggctctagtt
     8701 caggagcagc atactttcca gatagagtaa tgctaaatga taatgagagt tataatattg
     8761 aaacatctgt ttttcaagca ccacgtttta aaggagaagg aaaatatgct gtacaacaag
     8821 gtgcaggaat ggctgtaaca aaatctacta aagaagaaga atatgcatca gtagaatttt
     8881 taaaatggtt tacagataaa gagcctaata taaaattctc tattgcttct ggatatttac
     8941 cagtaaagaa aaaaacgaat gatttgaatg ttataaaaga agttatgaag aaagataatt
     9001 tgagtttatc tgataaagtt gaaggttcaa tagcctcatc tattgaacaa attaacagtt
     9061 gtaatttata cactaataaa gcatttgatg gtggaactaa agctagacaa atacttgaat
     9121 attcaatgct tgataaagct aaagaagatt caaataaaat aaatgaattg gtaaaaagtg
     9181 gaacttctaa gaaggatgcg gtagcacaat ttaataatga taagaatttt gaaacttggt
     9241 ttgaaagttt taaggctgag cttgaaaatg ctataaaaaa atagatgaat tggcagttag
     9301 tgtgtagaca agcacaaagg tataggtgga ataagtatgg aaaacaatct aagaaaaaac
     9361 aattcaataa tgaaaaagat gatatttccc ctaattattg ttatgctaat tcaaacaagt
     9421 ttattttgtg ccacaatttt atggggagga actattaaga aattaaatga taattcgttt
     9481 gacatcttaa atgaaagagt aattaatcgt aaaaattata tacaaaatga aatgttacaa
     9541 agatggtcaa atacaagtga aacggaagct acaattaatt cctctgtaaa aaaaatatta
     9601 aatgaaaaaa atgccttaat tgaggatgta ggcaacaatg aagaaattaa caaagatatt
     9661 ataaaaagca tatcaaatga tttaatatat cttttgagaa aaaactctgt tacaggagct
     9721 tttataattc taaatgacaa aaatattaac gataagccaa tagatagtaa aggtgaaata
     9781 agtaggactg gattgtatat aagggattta gaccctaagt ttaatccaaa tgataattct
     9841 gatttgttaa ttgagagagg ttcatcagat atagcaagaa gttttggaat atctatggac
     9901 agttattggg aacctaaatt ttcatttaaa agtgaaaatg agcaagaggg agatgatttt
     9961 ttttataaac catttagagc tgcaattgat aatccaaacg tatcaagttc taatttggga
    10021 tactggggaa gtaagttttt attgacaaat gcaggtgata ggcttatgac atatacagtt
    10081 ccccttattt atgaagatgg aactgtatat ggagtacttg gaatagattt aagtgtaaat
    10141 tatttaagag aattacttcc atactcagag ataaatggaa ataaaaaagg taattatatt
    10201 ttaggtgtag atagaaataa tgatatggcc tttgaaaatg taatttcaaa tggatactca
    10261 ttaaaagaaa aattttggga tggtttacat tctaatttag agcagaagga atcgtataaa
    10321 aatatatata agattaaaaa taaagataat gaatgtggta atggcaacat ttatgctagt
    10381 gtacaatact tagatttata taactccaat actccatttg aaaatgaacg ttgggctctt
    10441 ttgggtatta ttgaagaaaa aagtcttttg tcattttcaa aaaggataga gaaattagtt
    10501 ataatatcta tagcaatttc tttattaata ggtctaatag gtatttacat tgcatcaaga
    10561 ttatttacaa aaccaattgt tcttttagcc aacaaagtta gaaacagtaa tccatcaaaa
    10621 ccagtaaatc ttgataagat aaatatatct gaaatagatg aattatcttc agctatagag
    10681 ttactcagtg caaatgttgc agattcttca tcaaaattat cacagattat tgaaatggta
    10741 gatatgccaa tcggggcttt tgagttatac aaaaacgcag agaatgtttt ttgtacaaaa
    10801 gataaaaaac ttaagtggat aagaatgaat atctttgaag atgagctaaa ggttcttggt
    10861 gttgttgttg atgtcacaaa agaatttatt gagaaaagga agatagaata tgaaagagat
    10921 tttgatattt taacgaatct tttaaatagg agagcttttc attcacagat acaagaaaaa
    10981 tttaagaata aagatgaact taagatagca gcatttatta tgtgggattt agataatcta
    11041 aaatatatta atgatacata tggacatgat gctggtgatg aatatattag gtgtgctgca
    11101 gacatactta agaagtttat atcatggaat ggtatagtat ctagactttc aggtgatgag
    11161 ttttatgtat ttatatatgg atatgaaagt aaagatagta taagagatat tattaattct
    11221 gttaagaaac agatgaataa taccttatta aaacttcatg atggaacaga atttaaaata
    11281 agggcatcag caggaatttc ctggtaccct gatgattcaa ccaactatga tgaattaatg
    11341 agatacgcag attttgctat gtatcaaatt aaaaatacaa tcaaggggga agttagtgaa
    11401 tttgatatta atatatataa taaggattca ttcttattga gtaatagaga agaattaaac
    11461 aagttaattg atggtcaatt agttgagtat gcatttcagc ctattgtaga ttgtaaaggt
    11521 aaagtatttg catatgaggc acttatgaga cctaaaataa aaacttttag ttcaccaata
    11581 gatgtaatta gattagctcg ttcacagtct aaattatatc aaattgagcg tattacatgg
    11641 tttaaagcac ttaaatcatt taaaaaatac aaagaaaaat ttggcgattg caaactgttc
    11701 ttaaattcta ttcctaataa taatttatca aaagtagatt ttgaggaaat tgaagattta
    11761 tatggtgaat atttaaatag agtagtatta gaaataacag agaatgataa gattaatgaa
    11821 gattttattg agaaaaagaa agcatatata gaccattgga atatagaaat tgctttagat
    11881 gactttggga caggttacaa cggagatgct gtactattat atataatgcc agactatgta
    11941 aagattgata tgtctattgt aagaggtatt gataaagatg aaaacagaca gaaaatatta
    12001 aaaaatctta tctcatattc taacgaaaga aatattaaag tgatatcaga aggtgtagaa
    12061 acaaaaggtg aaatggaaac actgattaat cttggagtag attatatgca gggttactat
    12121 attagtaaac catcatttat acctgaagat ataagtgaca aaattaaaaa agagctcaag
    12181 atatgtcatg aaaataggaa tgatgtggtt taatagagga aaaattaaat ttatttttat
    12241 gaacttttag tttataatta aaaagaaaat aatataatta taaattaagg tggtggagct
    12301 aataatcaca acaaacatag atataagaaa gttaaatgaa aatcaattag aagcagttga
    12361 acatatagat gggccttgca tgatacttgc agggcctggt tcaggtaaga ccagagtaat
    12421 tacatataga atagccaata tggttgtaaa caaaaatata gcaccaacaa gaatcttggc
    12481 aataagtttt accaaggcat cttcaataga aatgaaaaat agagctttga atttaagtga
    12541 tgatataaga cttaataagg taacctatgg tacttttcac tctgtatttt ttaaaattct
    12601 aagatacttt gaaagataca atttagacag tatatttgat gaaaagtcaa agcgaatgac
    12661 aatcaaggca atcctaaaaa gtttaaatat agagaatgct gatgatgatg agaatatagg
    12721 acaagttata aatgaaatct cctatgtaaa aaatgaactt atggataaaa atgaatttaa
    12781 ttcagaagta ttaacaaaag atgagttttt aaaagtatac aacttatacg aagaacaaaa
    12841 atctaaagtt aacaaaatag attttgatga tatgcttata agaacttatt atttactttt
    12901 gaacaataag tctgcactag aaatggttag aaatgtgtat aaatatatat tgatagatga
    12961 atttcaagat ataaataaag tacagtttga agttttaaag ttaatttgta gcccattaaa
    13021 caatatattt gcagttggag atgaagacca aagtatatat ggatttagag gtgctagacc
    13081 agattttttg cttgaatttg aacaatactt taataatact aaaaaaataa tattagatat
    13141 aaattatagg tctaaaagtg agatagtgta tactgcaaat agacttatcg acaaaaataa
    13201 aaatagatat gaaaaagtca ttaagtgtag tcagggggat ggaggaagtg ttacctacat
    13261 ttctccacat gattcagagg aagaagcctt atatattgcc aaggaaataa ttgacgaaat
    13321 acaaaaagat tatgtagagt attcagattt tgcagtaatt tatagaacaa atatacagtc
    13381 aagagcatta gtagatgtat ttatggatat gaggattcca tttgtagtaa aagactcagt
    13441 aattacaata tatgaccatt gggcaagtca agatatacta gcttatttaa gaataggtat
    13501 taatccaaaa tcaaataaag attggttgag aattataaac aagcctttta gatatatatc
    13561 caaagacagt ataaatatgg ttaaggatga aaaagacttt ataacagcat taataaataa
    13621 gtgtaatctt catcctaaac aagttaaaac aataaatgat ttggaaatag atttaagtta
    13681 tttaaatact ttaaatccta aaaatgcaat atcttatatt agaactagtc ttgattatga
    13741 tagatacgtt ttagattatt gtagcaacag aaagataaaa actaatggac ttgtagagat
    13801 attaaatgaa cttgagagtt cagcaactaa ttttaatact gttacagagt ttttagagca
    13861 tattgataga gtaaagactg agttaacgga aaataacaag aataagcaga ctgagggtgt
    13921 catatttaca actatgcata gtgcaaaagg attagaattt agaaatgttt atattatagg
    13981 tgtaaatgaa ggaacaatac ctcatgaaaa atcatatgat atttgtaagg aagagaaaaa
    14041 agaagaacaa ttagaagaag aaagaagact tatgtatgtt gctattacaa gagctgaaga
    14101 aaagttatgt ataagctcta ctttaaataa gtatggtaag aaagttgata aatcattatt
    14161 tattaatgat ataaaatctc caacgaaaaa agaaattgac agtattggtg taggggataa
    14221 ggtataccat aaaaaatttc atgaaggaga aataattaaa aaagatggaa ttatgtttac
    14281 aataaagttt aaagatagag agcgaatctt ggatttgaaa acttgtttat tgaagaatat
    14341 aatttatact atttaattta gaatctaaat ttagatagaa atataatgta ttatttatta
    14401 tgtgcaagac aaatagatat gtgtaaaatg tattatttaa cttattagaa aaagaaataa
    14461 ggatttaata tattatttat agttgtttag aaaaagcaac aatgtggaaa atattattta
    14521 gtattattaa agaaaagcaa caatgtgaaa gatattattt agtgtaattt aagaaaaagc
    14581 aacaatgtga aagatattat ttagtattat taaagaaaga gcaacagtgt gaaagatact
    14641 atttagtatt attaaagaaa aagcaacagt gtgaaagata ttatttagtg taatttaaga
    14701 aaagcaacaa tgtgaaagat attatttagt attgtttaag aaaaagcaac aatgtgaaag
    14761 atattattta gtattattta agaaatagct aaaaatatga aatatactat ttataattac
    14821 aaaaataata tcaataaata taaacagtag tatttagata atttacaagt aaaaattatt
    14881 aaaaaaataa aaaacttatt gacaaaaata ttataaggaa gtacaattgt attaacaatt
    14941 taaggaaaca aatccaatga tagagaaagt agcatatatg gatttacagc gagttagggg
    15001 ttggtgtaag cctagcaatg aagtctatgt gaagagagct ctggaggaga ttatcagaaa
    15061 ctttagtatg ataattggtt tacccgcctt aagggattga gaggataagt acttacttat
    15121 caaaaagagt ggtaacgcgg atataattcg tctcttagct gtaaagctaa gggacttttt
    15181 tgatttaaaa ataaaaatat ccatacagct taaatgctaa cagtcacaaa ttttgaaagt
    15241 aatataagta tttatcaata agagtggtaa cgcggtaaag tcgtctctta gtcattaaag
    15301 actgagagac tttttttgta actcgaaaca tttaagtatt aaaatctgag ggaaattata
    15361 attaaaagag aggggtagtt aaaatgaaag tatttaaaaa attattaagt ttaggattag
    15421 tattaggatt gacattatcg ctagtaggat gtagtggtgg aggagaaaaa acaaagttag
    15481 aacaaataaa agacaatgga aaattagttg ttggtactag tgcagaattt cctccatttg
    15541 aatttcataa ggtagtagat ggaaaagatt caataaaagg attcgatata atgttggctg
    15601 aagaatttgc aaaagagctt ggtgtaaagg ttgaaattaa ggatatgtca tttgatggat
    15661 taataggtgc tttaaatgca gaccaagttg atattgttct tgcaggtatg tctccaacac
    15721 cagagagaga aaagagtgtt gatttttcag aattatatta tttaagtaga aatgcagtta
    15781 tagttaaaga tgcagatata gacaaagtaa aaactgaaga tgatttaaag aaacttagag
    15841 ttggagttca agcaggaagt atccaagaag aatatgtagt taacacttta aaaatgacaa
    15901 ctacaaaatc tttaaaagca atacctgatt taattacaga attaaaaaat ggaaacatag
    15961 atgcagttgt tactaatgaa gcagtttctt taataaatgt taaaaaatat gatggaataa
    16021 aaatggcaaa tactgaagtt ggtaaagatg taacagaagg tatggcagca gcaatcaaaa
    16081 aatctgacaa taacaaagat tttatagaat tattaaacaa aaagataaaa gaattacaag
    16141 atggtaagaa gatagaagaa tttttaaatg aagcatctac tgaagctgct tcaaattagt
    16201 aagaaagtta tgtgggagat gaaattatga gtttagattt tagtttttta agtagatttg
    16261 ggacttcttt tttggaagga acaggtgtga cagtatcaat ttctcttgtg gcattatgct
    16321 ttggatttat aataggtata attatctgta tggcaaaaat atcaaagagc aaagtattaa
    16381 gagcaatatc atcaatctat attgaggttt taagagggac accattatta gtacagatat
    16441 atatagtatg gtttggttta cctcaattag gaataagatt ccctatgttg tttggtatac
    16501 caagtgaatt tatagctagt gcatttgcat tatcagtaaa ctctggtgca tacgtggcag
    16561 agatacttag atcaggtata caatcagttg ataatggaca aatggaagca agtagatcat
    16621 taggattaaa ttattggtca actatgagat atataataat acctcaagct ataaaaaata
    16681 tattaccttc attagcaaat gagtttatta cacttgtaaa agaatcttca ataatatctg
    16741 taataggagt tgttgagata atgcgtactg ctgatatagt aaaaaatgca gcatttagag
    16801 cattagagcc attaatagta gcagcagcaa tttactttgt tataacattt actctatcaa
    16861 gattagttgg attattagaa aagaaactat ctgtgtctaa ctagatgaga gcatgtttag
    16921 catttaggga aaacatagaa tagtaaaaag gaaagtgata atatgattac aataaaaaat
    16981 ttaagtaaat cttttggaga tttaaatgta ttaaaaaata tagacttaga aatcgctaaa
    17041 ggcgaaataa tggttatagt aggtccaagt ggttctggta aaagtacact tcttagatgt
    17101 atgaatttac tagaaatacc tacaggtgga gaaataatat ttgaaggaaa aaatttggtc
    17161 gataaaaaaa ctaatattga tgaggttaga caaaatatag gtatggtgtt tcaaaacttt
    17221 aatttattcc cacataagac tattttagat aatataacat tagcaccaat taaattaaaa
    17281 aagatgacta aggaagaagc agaaaaaaaa gcagaaattt tgttgagtag agttggtctt
    17341 ttagataaaa aagattcata tccatcacaa ttatctggag gacaaaagca aagaatagca
    17401 atagctagag cacttgctat ggaaccagat atgatgttgt ttgatgagcc aacatctgct
    17461 cttgacccag aaatggtaaa cgaagttttg gatgttataa aagaacttgc taaagaaggt
    17521 atgacaatgg caattgtaac tcatgagatg ggatttgcta aggaagttgc agatagagtt
    17581 atttttatag atggaggaag tatacttgaa gataatactc cagaagaagt tttcggaaat
    17641 ccaaaacatg aaagaacaaa agcattttta gcaaaagtgc tataatatat aaaaaggatg
    17701 ttattgatat tagaaattca atacatcctt ttttagtgat ggttaatatt aattttaaaa
    17761 ttaaagttat attaatatta actttatttt tacgattttt tgcagaaaaa aaaagagctc
    17821 ggggtaagct ctttttttcc accgaattaa tttccgttac taatccgatt tttaataaat
    17881 ataaaatata tccttatgaa agggtattgg gaatagatat attaactaac tagggttacc
    17941 agggggataa ccttaattta ttataacaaa atgcgacaca aaaatcaata ttttttttga
    18001 aataaattat aaaagttatt tttataacaa gtaagcttat aaatttaact tttatagtaa
//
LOCUS       DACTGW010000065        17664 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.065, whole genome shotgun sequence.
ACCESSION   DACTGW010000065 DACTGW010000000
VERSION     DACTGW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.065"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            423..1553
                     /locus_tag="I9573_001701"
     CDS             423..1553
                     /locus_tag="I9573_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAT4796326.1"
                     /translation="MNNFEIKTRIKFGEGSLKVLQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKSIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILNNIK"
     gene            1794..2144
                     /locus_tag="I9573_001702"
     CDS             1794..2144
                     /locus_tag="I9573_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HAT4796327.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKRD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            2152..2601
                     /gene="eutP"
                     /locus_tag="I9573_001703"
     CDS             2152..2601
                     /gene="eutP"
                     /locus_tag="I9573_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HAT4796328.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDDIGLEKLFKYLEKSKNIDEV"
     gene            2703..3278
                     /locus_tag="I9573_001704"
     CDS             2703..3278
                     /locus_tag="I9573_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAT4796329.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            3271..4680
                     /locus_tag="I9573_001705"
     CDS             3271..4680
                     /locus_tag="I9573_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAT4796330.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSNENGTEVLITIPTQINI"
     gene            4864..6297
                     /gene="eutA"
                     /locus_tag="I9573_001706"
     CDS             4864..6297
                     /gene="eutA"
                     /locus_tag="I9573_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAT4796331.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQTIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSVDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            6315..7679
                     /locus_tag="I9573_001707"
     CDS             6315..7679
                     /locus_tag="I9573_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAT4796332.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFYKDGKLTELAGDAS
                     VLLG"
     gene            7692..8573
                     /locus_tag="I9573_001708"
     CDS             7692..8573
                     /locus_tag="I9573_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAT4796333.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKTTEVVVDNNAFI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSQETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            8593..9246
                     /gene="eutL"
                     /locus_tag="I9573_001709"
     CDS             8593..9246
                     /gene="eutL"
                     /locus_tag="I9573_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAT4796334.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEIAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            9257..9958
                     /locus_tag="I9573_001710"
     CDS             9257..9958
                     /locus_tag="I9573_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HAT4796335.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRIDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            9961..11430
                     /locus_tag="I9573_001711"
     CDS             9961..11430
                     /locus_tag="I9573_001711"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HAT4796336.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNIPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            11526..11813
                     /gene="eutM"
                     /locus_tag="I9573_001712"
     CDS             11526..11813
                     /gene="eutM"
                     /locus_tag="I9573_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HAT4796337.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            11940..12701
                     /locus_tag="I9573_001713"
     CDS             11940..12701
                     /locus_tag="I9573_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HAT4796338.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLEDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            12714..13343
                     /locus_tag="I9573_001714"
     CDS             12714..13343
                     /locus_tag="I9573_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HAT4796339.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            13385..14110
                     /locus_tag="I9573_001715"
     CDS             13385..14110
                     /locus_tag="I9573_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HAT4796340.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEILEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            14123..14395
                     /locus_tag="I9573_001716"
     CDS             14123..14395
                     /locus_tag="I9573_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HAT4796341.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            14388..14939
                     /locus_tag="I9573_001717"
     CDS             14388..14939
                     /locus_tag="I9573_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HAT4796342.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYVVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            14951..16042
                     /gene="eutH"
                     /locus_tag="I9573_001718"
     CDS             14951..16042
                     /gene="eutH"
                     /locus_tag="I9573_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HAT4796343.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NSGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLRKENV"
     gene            16050..16517
                     /locus_tag="I9573_001719"
     CDS             16050..16517
                     /locus_tag="I9573_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HAT4796344.1"
                     /translation="MSNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSIKL
                     PTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEIDYV
                     IDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(16637..16738)
                     /locus_tag="I9573_001720"
                     /pseudo
     CDS             complement(16637..16738)
                     /locus_tag="I9573_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            17033..17554
                     /locus_tag="I9573_001721"
     CDS             17033..17554
                     /locus_tag="I9573_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT4796345.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
BASE COUNT         7298 a         1965 c         3258 g         5143 t
ORIGIN      
        1 gaaatttaaa tattttaaaa gataattttt tggaactaat aggtataaca aaaatttata
       61 gggggattgc atatggaaat caatgttata gaaattttcc ctaaagataa agctaaactt
      121 aataaaatag aaatggataa agctagttgg tttgtaaata taataagtaa aaaatatcct
      181 aaagaagctt taaatgaagc atttagtatt ttagaaaaag aattaaatat aagtaaagct
      241 aatacataag ctttgattat tgattttata tactattggt tttatacgct atgacttgac
      301 attatattaa tcaaatagta tatttttaaa tatagcacaa tgaagtgtta aaagagcaat
      361 ggagctccat agataaagtc tatggagctt ttttgcgtat ttaacttaag gaggatagag
      421 ctttgaataa ctttgagatt aaaactagaa taaaatttgg agaaggttca ctaaaagttt
      481 tgcaagaatt aaaaaacaaa aatgtattaa taataacaga cccttttatg gtgaaatcaa
      541 aaacaataga taaaataaca tctaatttaa taaactcaac atatcaagtt tttgaagaaa
      601 tagtaccaga cccaccaata gaagtagtag tagcaggaat agaagcattt aaatctataa
      661 atccagacgt aataatagca ttaggtggtg gttctgcaat agatgcagca aaatctataa
      721 tggacttttc taaaaaaata ttaaaaatta acgaagtaga gtttatagca ataccaacaa
      781 caagtggaac aggttcagaa gtaacatcat ttgctgtaat aacagatagt caaaaaaaat
      841 gtaagtatcc attagtgagt gatgatttac ttccagatat ggcaatacta gacccagagc
      901 ttgtaaaaac tgtaccaaat tttataacag cagatacagg gatggatgta ttgacacatg
      961 ctatagaagc ttacgtatct acaaattcta atgacttttc agatgcacta gctgaaaaat
     1021 ctataaagtt agtatttaaa tacttaataa aagcatataa agatggaaat gacatagaag
     1081 ctcgagaaaa aatgcacaat gcatcttgtt tagcaggtat ggcatttaac caagcatcac
     1141 ttggcataaa tcattctatt gcacatgtat taggtggaaa attccatata ccacatggaa
     1201 gaacaaattc tatattactt cctcatgtaa tagaatataa ttcatcaata gtaggatata
     1261 ctaacacaac ttactctgat acagctaaaa aatatgcaca aatagcagaa ttagtaggac
     1321 ttgggagtgc taatataaga atatctgtta gaaatttagt aaatgaaata aagaaactac
     1381 aaaaagaaat gaatatgcca actaaattga cacaatgcaa attaagttta gaagatataa
     1441 ctaatgaaga aagagaaata gcaaaacttg ctataaaaga tacttgtaca ttaactaatc
     1501 ctaaggtacc aactgaagat gatataataa acatattaaa taacattaaa taactacatt
     1561 ttattacatg taaatataat attatacaaa tagggaaaat gtttttaaaa atataaatta
     1621 tatattgaca aatataaaac ataaaactat aatgaaagta tcacaaaggc gtgatacttt
     1681 agtagaaagc aatggagctt cttaggaata ttaatattat tcttatggag ctccttttat
     1741 tttaagcaaa tagcttttta ataaactata ttcaaagaaa gcagggaata aatttgactg
     1801 aagaatctaa gcaaagagtt attcaagaat atgttcctgg aaaacaggtt acattagcac
     1861 atatcatagc taaccctaat gaagacatat ataagaagtt aggattagta cttgataaaa
     1921 gagatgcaat aggaatactt acaataacac caagtgaagc gtcaataata gcagcagatg
     1981 ttgctacaaa ggcttcaaat gtatctttag gttttataga tagatttagt ggttcagtag
     2041 taatatcagg tgatgtaagt tctgtagaat cagcgttaaa tgatgtttta gaagtcttag
     2101 gaaatatgtt aaacttctca tctacaaaaa tcacaaggac ataataatac aatgaagaat
     2161 ataatattta tgggaaagac aggtagtggt aaaactacct tatgtcaaaa actagaccag
     2221 ttagaattaa agtataaaaa aactcaatca gtggaacttt ataataatgc aatagatact
     2281 cctggtgagt atatggaaaa tagaaaccta tataatgcct taataacaac atcagcagat
     2341 gctaaaatta tagcaatagt gtatgatgcc acacaagaag aaaattatat agcaccaggt
     2401 tttgccagta tgttttgcaa agacgtaata ggaattataa ctaaaataaa taaagctact
     2461 gaaaacgaag tcaatatagc ttatgaaaga ctatcactag caggagcaag tcaaatcttt
     2521 aaaatagaca cagttgatga tattggactt gaaaaattat ttaaatatct agaaaaatct
     2581 aaaaatatag atgaagttta aggggaaata tttttttatt caaaaatatt attaaagtat
     2641 gataaaataa aaaaatgtat aaactatttt aagaagattt aatctttaag cgaggtagga
     2701 acatggggaa aaaaatacta ttggttgatg atgaaccact gttaaggatg gatacaaaag
     2761 atatattaga aagtcatggc tatgaaataa taggcgaagc aagtgatgga tttgaagctg
     2821 ttgaaatgtg taaaaaacac aaacctgatt tagttataat ggatatagag atgccaatac
     2881 ttgatggtat aaaggcaggc aaaataatat gtaaagaaaa tctagctggt ggagtacttt
     2941 tattaacttc atttgaagat aaagaatacg tagaaaaggc taaaagtata ggtgcttatg
     3001 gatatttagt aaaaacagca agtgaaaaat cacttatccc aaccatagaa atgtgtttaa
     3061 ataaggtaaa tgaatttgaa aaaatgaaaa aagatttaga aaaagtaaat tctaaattga
     3121 atgaaagaaa attagtagaa agagcaaaag gtatacttat aagagagttt aagatttctg
     3181 aggatgaagc atacactaga ataagaaaat taagtatgga taaaagaact acaatgatgg
     3241 aaattgccaa aatgattata ataggatacg atgactaata cgataaaaaa aatatcaact
     3301 gcacaaacat acttaaataa taacgaaatt gaaaaaatta tagaagtatc aaaatcctta
     3361 gatttaatgg cttcttttta tgaggctgat gtttttatag atgtactaag taaatttgat
     3421 gatgaagcaa ttgttgtggc acatggtaga cctaaagaaa agtcaatgta ctgtgaaaat
     3481 gttataggta aaaaggcttt aattgaaaat gaaccaggag ttataaagtg cttaaaaaca
     3541 ggagaaagag taagagatat taaagcttta acacaggaat ataaactggt caaacaaaat
     3601 attcagccaa taacactaga tgacaaagtt ttagcagtac taatagttga aaaagatata
     3661 agtagtgaag ttaagaatga gtttaatgct agtgaagacg aagaaaataa aaatttacat
     3721 cagtttataa aacttatcgg aaataatgac tttttagttg ataatttaaa ttcagccata
     3781 ttaatatttg ataaaaatgg aaaattaaaa attaaaaatc gtattgcaac tgagatatac
     3841 aaaaatcttg ggtttggata tgatatacaa gatatgcatt acaatgattt agatatagaa
     3901 accaacacct ttgaaaatat attaatggat gaaaattatg atgagacaaa tgaagtttca
     3961 ataggtaata tgttttttaa aataaagacc atatacatca aagatgatga atttaaggtt
     4021 tgcaaaatag tacaagatat aagtgattta aagagaaaag aagaagaatt agttttaaaa
     4081 acagtggcta taagagaagc tcatcataga gtaaaaaata atcttcatac agtaatatct
     4141 ataataaaaa aacaaagtag attatctcaa aatgatgaag taaagcagtg tttagataac
     4201 ataataaaca gagtatttgc aatactttca actcattatt tactatctaa agaagttgat
     4261 aataatatat ctatacgaga aggtatcaat ttattagtgt caaatattca aggtggctat
     4321 ttatttagta aggatataga tatacgtata actggtgaag attttaaaat tagtggagaa
     4381 aaagcaacag caatactttt ggttataaat gaaatactac aaaattgtta tgaccatgca
     4441 ttttcggata gagaacatgg tactatacaa atattaataa ataaagataa cgactataga
     4501 aatattgcta ttatagatga tggaattgga tttgatagta ataatataaa taaaaatagc
     4561 ttagggacat atataataga tagttatata acacagatgt taaaaggaaa tatagagaga
     4621 aaaagtaatg agaatggtac agaagtatta attactatac ctacacaaat taatatttaa
     4681 ataataactc atctacaaag tagtagtttt aattgagcaa agaggcttaa ggttattaat
     4741 agattaagtt aattgaatgt atttataaaa attataagta tatattcaat ttaacaggta
     4801 tttattataa ctttaagcct ctttgcttgt ttaagacata cttaaatttt aggaggtgag
     4861 aaaatgagag aagagttatt aagtgttggt attgacattg gaacaagtac tactcagtta
     4921 attttttcta aattaatagt agaaaatact gaatcaaatt tttcggtacc tagaataagt
     4981 atagtagaca aagaaattat ttataaaagc aaagtttatt tcacccctct tatttcaaat
     5041 aatgaaataa atggtcaaca aataaaagat atagttgaaa atgaatataa aaaagcaaat
     5101 atatctaaag aggatataca tacaggagca gtaataataa ctggtgaaac agctagaaaa
     5161 gaaaatgcaa atgatgtact tcatactttg agtggatttg ctggggactt cgttgttgca
     5221 acagcaggac ctgatttaga aagtataata tcaggaaaag gagctggagc tcatatttat
     5281 tcaaaagaac attcaactac aattgtaaat atagacatag gtgggggaac tagtaacata
     5341 gcctcattta aaaggggaga agtaaccgat actgggtgtt tggatatagg tggaagactt
     5401 gtgaagatag ataaaataag taaaaaaata acttatatat cacctaaaat agataaaatc
     5461 ataaaagata ataatttaaa tcttcaagta ggcacaatgg ctactgtaga aaatacaaag
     5521 ccaatagtgg atataatggt tgacttatta atggaaagca taggattaaa gaaacagaca
     5581 cctttgtttg attttataat aaccaataaa tcaataagta tagatgaaaa tattaaaaat
     5641 gtttcattct ctggtggagt agctgattat atttactatg atggagaaat aaaagattat
     5701 tttgaatatg gtgacttggg aattttactt gggcaaacaa taaaaaattg tgaatacctt
     5761 aaaaaagtaa atgtagttaa atctatggaa acaataagag ccacagtggt tggggcaggt
     5821 tctcatacta ctgaagtaag tggaagtaca ataacttaca ctagcgaaaa gtttccactt
     5881 aagaacttgc cagttttaaa attatcagtt gatgaagaaa caggtacttc aaaacaaatc
     5941 agtgaagcta taaaaaataa acttaattgg tttaaactag aaaatgattt gcagaaagtt
     6001 gctatagcca tgataggaaa gaaaaatcca acctttttag atatacaaga gtatgctaaa
     6061 gggtttgtag atggaatgca acaaatcata gaaaatgatt taccttttat agtaattgtt
     6121 gaaaatgata tggcaaaagt gttaggtcaa gcaatatatg cattattaaa ttatgaaaaa
     6181 gaaattgttt gtatagacag tgtcaaggtt gaaaatggag actatatcga cattggaaaa
     6241 cctatagttg aaggtaaagt tttacctgtg gtaataaaaa cattagtttt taattaatag
     6301 aaggaggtaa gtaaatgatt ttaaatacaa aattatttgg acgtacttat cagtttaaag
     6361 gcttatatga tgtaatggct aaagctaatg aagaaaaatc aggagataaa ttagcaggtc
     6421 ttgcagcaga atcagctgaa gaaagaatag cagcaaaagt tgttttatca cacataaaat
     6481 tggaggatat aagaaataat cctgcagtgg catatgaaga agatgaagta acaagaataa
     6541 tacaagatgc tgtaaatgaa aaagtttatg aagaaataaa aaattggaca gtatctgaac
     6601 ttagagaatg gctattggat tcaaatacta caagtcttga tataagaaga atatcaagag
     6661 gattaacttc tgaaatgata gcagcagttg caaaattaat gtcaaatatg gacttagttt
     6721 atgcagctaa aaagataaag gttactgctc actgtaatac aactatagga gaacaaggaa
     6781 cattatcagc aagacttcaa cctaatcatc caacagacga cccagatgga atacttgcat
     6841 cacttcttga aggtttaact tttggtatag gagatgcagt acttggatta aatccagttg
     6901 atgactctgt tgagagcgtt actaaagtat taaaaagatt tgaagaagta aaacaaaaat
     6961 atcagatacc aactcaaaca tgtgtattgg cacacgttac tacacaaatg gaagcaataa
     7021 gaaatggtgc tcctgcagat ttaatattcc aatctatagc aggttctgaa aaaggaaatg
     7081 aagcatttgg atttaatggc gacactatag aagaagctag acagttagct ttaaaacacg
     7141 gtacagctac aggacctaac gttatgtatt ttgaaactgg acaaggttca gaattatctt
     7201 cagaagcaca tcatggaata gaccaagtta ctatggaagc tagatgctat ggatttgcta
     7261 aaaagtatga accattctta gtaaatacag ttgttgggtt tataggacct gagtacttat
     7321 atgatgctag agaagttgta agagcaggac ttgaagacca ctttatgggt aaactacatg
     7381 gcttaccaat gggtgttgat gtttgttaca ctaaccatat gaaggctgac caaaatgata
     7441 tggaaaactt agccatacta ttaacaacag caggatgtac gtattttatg ggaatacctc
     7501 atggagatga tgttatgctt aactatcaaa caacaggata ccatgaaaca gcatcactta
     7561 gagaaatgtt tggactaaca gcaataaaag aatttgatgc ttggatggaa aagatgggat
     7621 tctataaaga tggtaagtta acagagttgg cgggagacgc atctgtatta ttaggatagg
     7681 aggtatttaa tatgaatgag aaggatttaa aagcattggt agaacaatta gttggacaaa
     7741 tggttggaga attagatact aatgttgtat ctgagactgt taaaaagaca actgaagttg
     7801 tagtagacaa caatgctttt atagatgata taacagaagt tgatataaga aagcaattac
     7861 tagtaaaaaa tcctaaggat gcagaagctt atttagatat gaaagcaaaa acacctgcaa
     7921 gattaggtat aggaagagct ggtactagat ataaaacaga aacagtttta agatttagag
     7981 cagaccatgc tgctgcacaa gatgctgtat tctcttatgt agatgaagaa tttataaaag
     8041 aaaataatat gtttgcagtg gaaactttat gtaaagataa agatgaatac ttaacaagac
     8101 cagatttggg aagaaaattt tctcaagaaa ctataaataa tataaaatct aaatttggta
     8161 caaatcaaaa ggttttaata ttagttggag atgggcttag ctcagctgca atagaggcaa
     8221 atttaaaaga ctgtgtgcca gctataaaac agggtctaaa aatgtatggt atagattcaa
     8281 gtgaaatttt atttgttaaa cattgtagag ttggtgctat ggaccactta ggtgaagaat
     8341 taggttgtga agttatatgt atgttggttg gagaaagacc aggattagtt actgctgaat
     8401 caatgtcagc atatatagct tataagccat atataggaat ggcagaagca aaaagaacag
     8461 ttatatcaaa tattcacaaa ggtggaacaa ctgcagttga agcaggtgct catatagctg
     8521 aacttataaa aactatgtta gataaaaaag cttcaggaat agaccttaaa taaagtcgag
     8581 ggagtgtaga atatgaaaaa tgatgtaatt cgtcctaata tactaggaat taaaataatt
     8641 tcgaatataa gtccagaaat ggcacaaaaa ctagaattaa aatcacatca caaaagttta
     8701 ggtttaataa ctgcagactg tgatgatgtt acgtatacag ctcttgacga agcaactaaa
     8761 gcttcagagg ttgatgttgt atatgcaaaa tcaatgtatg caggtgctgc aaatgcttca
     8821 acaaaacttg ctggtgaagt attaggaata atagcaggcc caagcccagc agaagtaaga
     8881 agtggtctaa atgcagtagt tgattttcta gagtatggag caacttttat aagtgcaaat
     8941 gatgatgatt caatagcata ctatgctcac tgcgtatcaa gaacaggtac atacctttca
     9001 gagatagcag gaataagaga aggtgaagca ttagcttatt tagtagcacc accacttgaa
     9061 gcaatgtatg cactagatgc agcaatgaaa gctgcagatg ttaagatgtg tgaattattt
     9121 gcacctccaa ctgaaacaaa ctttggtgga gcattactta caggttctca atcggcttgt
     9181 aaagcagcat gtgatgcatt tgcagaagca gtaaaatcag ttgcagacaa tccaacagga
     9241 ttttaaggat agttagatgg atgctatagg cttaatagaa gtaataggat atgttgctgc
     9301 aatagaagct tctgatgctt gtttaaaatc tgcaaatgtt aatattgtaa gaattgataa
     9361 ggttggggca ggtattgtta ctttaacaat atgtggagat gtcggtgctg taaaagctgc
     9421 tttagaagca ggtgaaatag cagcttccag agttggtaca ttaagaacat cacatattat
     9481 accgagaatc cataatgaag taacaaatgc tttattcaaa actaaagaac ctaaagtctg
     9541 tgaagtttca gaagataaag aaaaaaactt tgagattaca ttggaaataa ctgataatgt
     9601 atcgaaaaat ttatcagaaa aagaaaatgt aattttagat gtcaatgaaa acagtggaga
     9661 tactgaaaat ttatatagtg atatagataa aacaaacaaa aaaattgaaa cagttgatga
     9721 tagtttaaca gaagctaatg aggaaaaaat agaaaatact aatgataaag aagtattgga
     9781 acaagttaaa atagaaaatt ccgattttga tagagatgac ctatcaaaga acagtgtaaa
     9841 aaaacttaaa gctatggcta agaaattaga ttcttcaata acttataaag atttaaatgt
     9901 tcttaaaaaa gaagaattaa taaacttagt taaaaagctt agcagaaggg atagatagtt
     9961 atgacacttt tagataaaga tttagtatct atacaagaag tcagaactct tgtaagaaga
    10021 tctaaagtgg ctcaagagaa actttctaaa atgagtcaag agcaaataga taaaatagta
    10081 aaagctgttg tagatgcagc atttaataat gctactaaat tagctaaaat ggcaaatgaa
    10141 gaaactggat ttggaaaatg ggaagataaa gtaattaaaa atacttttgc atctatggga
    10201 gtttatgaaa ctttaaagaa cacaaaaact attggaataa ttggtgaaga taaagaagcc
    10261 aatacgatag atatagctac accagttgga gttgtggcag gtcttatacc ttcaacaaac
    10321 ccaacatcaa cagtaatata caaaactata atatctctaa aagcaggaaa tactatagtt
    10381 ttctctccac atccaaatgc aaaaaactgc atacttgaaa cagttaatat aataagtgag
    10441 gctgctataa aggctggagc tccagaggat tcaatattat gtataactaa tccaagtata
    10501 gttggaacca atgaattgat gaaacataaa gatacaaact taatactagc aactggagga
    10561 gaagcaatgg ttagagctgc ttactcttca ggaacaccag ctattggagt tggaccagga
    10621 aatggaccag catttataga taaatcagca aatgttaaat tagcaatcaa aagaataata
    10681 gattctaaaa cttttgataa tggtgttata tgtgcatcag agcaatctat agtaatagaa
    10741 aaatctatgg aagaaactgt tttaaaagag cttaaaaatc aaggtgcata tgttttaaat
    10801 gaagaggaaa gtgaaagatt agcaaaattc atattaagag ctaatggtac tatgaatcca
    10861 caaatagttg gtaaaagtgt tgaggtaata gcaaatttag cagacttaac aaatatacct
    10921 aaaaatacaa aagttctagt tgcaagagaa aataaagttg gtcacaatgt agcctattca
    10981 agagaaaaac ttacaccaat actagcgttt tatgttgaag aaaatgtaga agctattatg
    11041 aacagatgta gagaaatatt gttaaatgaa ggtgcaggtc atacattctc aatgcatgta
    11101 aatgatgaag aattggtaaa aagatttgcc cttaatatgc cagtatcaag aatacttgtg
    11161 aatactcctt cagcacttgg aggaataggt gcatcaacaa accttgtacc agcactaaca
    11221 cttggttgtg gtgcaatagg tggaagttca acttcaaaca atataggacc acttgattta
    11281 ataaatgtta aaagagtagc ttatggaatc aaagaaatag aagaacttgg aagagataaa
    11341 tctcaagttg aaaaatcaat gtttgactca atggataaag aagaattaat aaatcttctt
    11401 gttcaaaaac tagtcaaaga actagcataa ctaaaataaa caatgaaact tatggctatc
    11461 gctataaatt tataaaaata taaactataa aaaactataa aattaaattt ttggaggata
    11521 agattatggc aagtgcaaac gcattaggaa tgatagaaac taaaggatta gtaggagcaa
    11581 tagaagcagc agatgcaatg gttaaagctg caaatgtaca attagtagga aaagagcaag
    11641 taggtggagg attagttact gtaatggtaa gaggagatgt tggagctgtt aaagctgcta
    11701 cagatgcagg agctgcagct gccgagagag ttggagaact aatatcagtt catgttatac
    11761 caagaccaca ctttgaagta gatgctatat taccaaaggt atcagctgag taatataaga
    11821 ataattttaa aatttaaatt ttgattgaaa aagaggctat ctcaaaaatg agtcttacta
    11881 ataaactcaa tgtattttga ggtagtcttt tttatacatc taataggaga tgtgataaaa
    11941 tgggtgtttt aacagaaaac gaagtaaaaa gaaaaattgg aaaaaacaaa aataatataa
    12001 ctgaatttta tatagaaaaa ggtcaaataa taactccttc tgcaaaatct tatttagctg
    12061 agagaagtat tgagcttaaa tatgttgata aactttcaga tgtaaacaat gaacctaaag
    12121 aagttgtaaa agaagtagtt aaagagaaaa aatataaata tactactgta tttggcgcta
    12181 aattggatga aaaaccagaa catatgactc aattaaatgg aaatctttta gtttttaaag
    12241 accataaaag aataatattg agaggaaaaa tagattctct tgaatcagaa atactaaagg
    12301 tacagatact ttgtcagaaa ctagatatgc caaagcttgt taaggaattg caagaagtat
    12361 tagactttgt aagaaatata atgagatgtg aagttttaga tgaaaagcta gaagacttta
    12421 aacttttagg attaactcct caagaactta gagaacaatc tcactatcct aaaaaatact
    12481 ttggtatagg acatgaattc ccaagttatg aaatgggaga agtagtagta gctataaata
    12541 gtataagaag taatacacga gaggttgaac ttgcagccta tgaagcattt aagggtgagt
    12601 atggaaatgt tcacagagaa gatttaataa aggcacttaa tagattatcg tcagtatttt
    12661 ggataatgat atataaggtt agaactaata agtataagta gttggaggac ataatggaga
    12721 atctattaaa tgaaatagtg gaagaagtaa taactagagt taaaaaagaa gcatttatag
    12781 aagttgaagc atcaggaaga cacgttcact taagtagaga agatgtcgat aaattatttg
    12841 gagaaggata tactcttaca aaactaaaag acctatctca accaggtcaa tatgcatgta
    12901 aggaaagagt tacaataact ggtcctaaag gaagtataaa aaatgtagtt gtacttggac
    12961 catgtagaaa tgaaactcag gtagaaatat ctttaacaga tggttcaact cttggactaa
    13021 aagctcctat aaaacaaagt ggagacttag aaggtacttt aagtattaaa atatcaacac
    13081 aatatggtga agttgaactt gataaggggt taatggttgc taaaagacat atacacatga
    13141 ctcctaaaga tgctgaaaaa ttcaatgtat gtgataaaga gatagttgaa gccaaggtga
    13201 tgggtcaaag acctttaaca tttgatgatg tagtaataag agtgagtgac agttttaaaa
    13261 cttatatgca tatagactat gatgaagcca atgcatgtgg ttattcaaaa ggaactgttg
    13321 ctaaaataat aaagaaagca taaatttaac ctactatata aattacctct agggaggatt
    13381 taatatgaat tatgataacc ttgtagattt aataacggaa gaaatctata aaaaaataaa
    13441 tagtaatgaa ataaaaatat ctaataaacc taaagctgtg attgtatttg aacaagataa
    13501 caataaattt aacttattaa aagatgaatt tgaaatacta gaatttgata aaagtataag
    13561 ggaatgtgaa atagtgatag tttcaagact ttgtatgagg gggctatcga atatagcact
    13621 tggaaattca acaagtgatg aagaaagatt tatactaaaa atgattatga aaggcaaaaa
    13681 agtatatgtc ctagatgaag gaattgaata caaaaaatat aaggatactg caccaaaagc
    13741 attatataag aaatttatgt catttgaaga tgaaatatgt aaatttggtg tagaaattat
    13801 aaaagactta aatgttataa ctaaaaataa gttaaatata aagaaagaac tagaaagtac
    13861 tagaggttct aaaaaagaag atacaaaagt tttagataaa gatgttttaa atttaaccag
    13921 tgtagaaagt gtaaaaatag ataatgaatt tagtttggat ttaaggaata aaaaacttat
    13981 atcggaagct gacttaagaa aaccaactat aaatggagtt aaaaacatat tagttaataa
    14041 aaaaagtata ataacaccat tagctgtaga ttttatgaga atacaccatc tcaaactaaa
    14101 gaaactgtaa ggaggtaatt ttatggatat aggaaaagtt gtaggtaatg tttgggctac
    14161 aagaaaagat gaaaaattat gtggccaaaa acttttagtt gtaaaaattt taaaaactaa
    14221 agacacatac aaggaaggct tcatggttgc agccgataat gcaggagcag gaaatggaga
    14281 cttagttctt atatccaaag gttcctcagc tagagagtcg attgaaaaat ctcatgttcc
    14341 agtagatgca actatagttg gagtagtcga ttcgtttgag gtgtttgatg aataaaagta
    14401 taggagctat agagtttaaa agcattgcta aaggtataga agtatccaat gaaatgataa
    14461 agaaatcttc agttgatgta ttgtacttaa aaagtatttg cccaggtaaa tttctaataa
    14521 tagtaggtgg agaaacttct tatataaatg aatgtgtaga ttatggaata aaacttggag
    14581 aaggatatat tgtagataac ttcgtaataa atgcaatttc acaagaaata ttagatggat
    14641 ttaaaaataa gtatcaaaag ctagatagta tagtctctat aggcgttgtt gaaagtagta
    14701 aagtatgcgc tggaataaaa atgcttgata agactttaaa atctggcgac ttagtattag
    14761 taaaacttca attatctttt gctataggtg gcaaattagt atatgtagta gcaggagatt
    14821 taagtagttt agaatatgca ttaaaagaaa gtgaaaatgt agtaagagaa aaagaagtaa
    14881 tatataagac tgtaatacca tcagtagaca gtcaaataat aaaaagtcta ataaaataat
    14941 ggggggaatt atgagtataa acgaaattat aatctatgta atggtagtct tcatggtact
    15001 tggagctata gataaatgta taggtaacaa atttggtctt ggagaacaat ttgaagaagg
    15061 tataatggca atggggtcac ttgcagttgc aatggttggt gttatatgtt tagcaccagt
    15121 tcttgctgat gtattaagac caattgtagt accagtattt aatatacttg gagctgaccc
    15181 agctatgttt gctggaagtt tattagctaa tgatatgggt ggtgcgccac ttgcacttga
    15241 acttgcgaaa gaccctaatt ctgggttatt tggtggatta atagtagggg ctatgatggg
    15301 acctacaata gtatttataa taccagttgc gcttggaata attgaaaaag aagaccaaaa
    15361 attcttagca acaggaatac ttgcaggtat aataacaata cctattggag cattcgttgg
    15421 tggattagtt gcaggattcg aggtaatgat ggttcttaga aacttagtgc caataataat
    15481 atttgcagta gttatagcac ttggattatt aaaattcgaa aatgctatga taaaaggatt
    15541 cacatacttt ggaaaaggtg tagtaatagt tataacttta ggacttgctg ctgccatagt
    15601 tgaagcttta acaggaatag ttgtaatacc tggaatgaaa ccaatcagtg aaggtataga
    15661 aatagttgga gatatagcaa tagttttagc aggagcattc ccattagtgt ttgttataac
    15721 aaaggtattt aataaaccat taatggcttt aggaaaagtt cttggaatga atgatgtatc
    15781 agcagcaggc cttgttgcaa gtttagctaa ctgtatacca atgtttggta tgatgaaaga
    15841 tatggacaat agaggaaaaa taataaatgt agcattctct gtatcagcag cattcgtatt
    15901 tggagaccac ttaggattta cggcaggatt taattctaat atgataacac caatgatagt
    15961 tgctaaatta gttggtggta taacagcagt attgttagct atggttatcg caaataagac
    16021 actaaggaag gagaatgtat aagatggata tatcaaatat agataaaaat ttaattgaaa
    16081 cattagtaag acaaataata gaagaaaaaa taagtggaac taaagacaca gtagattttg
    16141 ttagaaataa agacattagt ggaataactt ctataaagtt accaactgta aaagttagtg
    16201 aatctgatag attagataca gggaatccaa gtgatgtagt ttacacaaaa gatttattta
    16261 cattagagga aagcccaaga ttaggttgtg gtatgatgga aatgaaagaa acaacttttg
    16321 attggactct aaactatgat gaaatagatt atgtaataga tggaacttta gatataataa
    16381 tagatggaag aaaggtttct gcttcttcag gtgaattaat attcatacca aaaggaagta
    16441 agatacaatt ttctgtacct gattatgcta gatttatata tgtaacatat ccggcagatt
    16501 gggcatctca aaattaataa aaatcaagtt aagttaataa taatcaaata aataatttaa
    16561 aatacaaact cttaaataaa cacacatatt taaaaaagct atgaattaaa attcatagct
    16621 ttttttataa agaatgttat attttagacg tattttttct atatttactg ggagacatat
    16681 tatatttttt attaaataat ctaataaaat gctctacatt tggataacca atactatttt
    16741 actgataatc ttttccttat cactaatctt acattttaaa atattatttt tactcgtaat
    16801 attatcataa gaatttgttt cttatttaaa actaattaga attataaagt ttattgccat
    16861 gttattaata aaataaatcc agttaaatcg agtgctagtt aaagtaataa gaaactttta
    16921 caatggtttc ctttatcgat tgaaataaaa gttaatatgg tataatatct aaagagagaa
    16981 tcttatcaat tagaatatgc atagaaacaa gtggcttaaa aggagacgtt atatggaaga
    17041 gatagtaaag aaggaacaag caagtatgaa tgatattttt ttgtatctta aagctaaatc
    17101 aaactcatta tataaatttg taatattata taatgagttt atcaataaac ctaaagatta
    17161 tggaactggg gaacttataa acatggttga agtacatatg ctaacagcga ttgaagaaaa
    17221 tccaggaata agtccaactc agttagctaa aatatggaac agaactaaag gtgcaatatc
    17281 tcaaactata tctaaattgg agaaaaaggg ttatataact aaagaaaaga aagaaggaaa
    17341 tgcaaaaact atattactat atccaaccac atcaggagta gagttaagta aggcacataa
    17401 actatatgac accatagatg ttacaagaac atttgaatac ttaatcaagg agtgtacatt
    17461 agaggagata gatagttttt ataaagtaat tgatttgttt attaatttat tagaagaatc
    17521 tccgcaggaa aataatgatt ctgaaaaagt gtagctaaga ttgaagttga ataaagagaa
    17581 atagatatta tagaagtagt acttaagtac acattatata aaaatataat aggtatagga
    17641 ttaaaaagtt tagtggttta aagt
//
LOCUS       DACTGW010000066        17126 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.066, whole genome shotgun sequence.
ACCESSION   DACTGW010000066 DACTGW010000000
VERSION     DACTGW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17126
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.066"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(287..679)
                     /locus_tag="I9573_001722"
     CDS             complement(287..679)
                     /locus_tag="I9573_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAT4796346.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            1247..1609
                     /locus_tag="I9573_001723"
     CDS             1247..1609
                     /locus_tag="I9573_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796347.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1593..2309
                     /locus_tag="I9573_001724"
     CDS             1593..2309
                     /locus_tag="I9573_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796348.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVVEFTEEDEEYEEELKQNIIE
                     NIKKNFDEKKLVQVYKYRGV"
     gene            2329..2748
                     /locus_tag="I9573_001725"
     CDS             2329..2748
                     /locus_tag="I9573_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796349.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2738..3262
                     /locus_tag="I9573_001726"
     CDS             2738..3262
                     /locus_tag="I9573_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796350.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYVDENNNEESISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVLITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            3574..4131
                     /gene="efp"
                     /locus_tag="I9573_001727"
     CDS             3574..4131
                     /gene="efp"
                     /locus_tag="I9573_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAT4796351.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            4223..4678
                     /locus_tag="I9573_001728"
     CDS             4223..4678
                     /locus_tag="I9573_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAT4796352.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCPSCDDRE"
     gene            4841..5551
                     /locus_tag="I9573_001729"
     CDS             4841..5551
                     /locus_tag="I9573_001729"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAT4796353.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5605..6675
                     /locus_tag="I9573_001730"
     CDS             5605..6675
                     /locus_tag="I9573_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT4796354.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6703..10257
                     /gene="smc"
                     /locus_tag="I9573_001731"
     CDS             6703..10257
                     /gene="smc"
                     /locus_tag="I9573_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAT4796355.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKVSLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEAYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSIATEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIGSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            10284..11564
                     /gene="ftsY"
                     /locus_tag="I9573_001732"
     CDS             10284..11564
                     /gene="ftsY"
                     /locus_tag="I9573_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAT4796356.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDSLENLEETIFSG
                     LEEEVIDKVEDIEEKTEESVNNEGTEEIENFEKIEVEMDSNAKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11667..12035
                     /locus_tag="I9573_001733"
     CDS             11667..12035
                     /locus_tag="I9573_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAT4796357.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            12032..13384
                     /gene="ffh"
                     /locus_tag="I9573_001734"
     CDS             12032..13384
                     /gene="ffh"
                     /locus_tag="I9573_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAT4796358.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMAELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13422..13694
                     /gene="rpsP"
                     /locus_tag="I9573_001735"
     CDS             13422..13694
                     /gene="rpsP"
                     /locus_tag="I9573_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAT4796359.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13759..13986
                     /locus_tag="I9573_001736"
     CDS             13759..13986
                     /locus_tag="I9573_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAT4796360.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            14094..14609
                     /gene="rimM"
                     /locus_tag="I9573_001737"
     CDS             14094..14609
                     /gene="rimM"
                     /locus_tag="I9573_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HAT4796361.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14618..15313
                     /gene="trmD"
                     /locus_tag="I9573_001738"
     CDS             14618..15313
                     /gene="trmD"
                     /locus_tag="I9573_001738"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAT4796362.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVYIYNIRDFSNN
                     KHKKVDDYPFGGGVGMVMTPQPIYDTYKHIITTHNISNPSVIYLTPKGKVYNQSIAKQ
                     MSLKEDIILLCGHYEGVDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15498..15848
                     /gene="rplS"
                     /locus_tag="I9573_001739"
     CDS             15498..15848
                     /gene="rplS"
                     /locus_tag="I9573_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAT4796363.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            16196..17065
                     /gene="ylqF"
                     /locus_tag="I9573_001740"
     CDS             16196..17065
                     /gene="ylqF"
                     /locus_tag="I9573_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAT4796364.1"
                     /translation="MDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELLDARIPFSSK
                     NPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIKGVGINNIIE
                     ECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQTGDKPGVTK
                     GKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETLALRLIEKLM
                     KIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIAATVLNEFRDGKI
                     GNITLEVPENVKR"
BASE COUNT         7376 a         1539 c         3180 g         5031 t
ORIGIN      
        1 aaaatgacaa aataaaaaat actatttaaa tagagattaa aaaattgatg cgaagtattt
       61 gtttgctata tgaataacaa tttatttaga agttttgatt tgacactgta taataataaa
      121 gtaattgcta aaaaagtttg atgatttgga tgtattttta gaaattagaa aaatatatac
      181 ttactgacta atgataatct tatatatatt ttagtttgag tgatttaaga aaagtgtttt
      241 gaaaggggat tattttatag aataaatata tatatatagt agaaaattac atataaagag
      301 tagtatttat ttcattacct tctttatctg ttccgatggg gtcttgtaga gatacttgag
      361 ttttattttt tttgttagat ctaatattca ttaaaatctc attttcaata catctagaag
      421 catatgttgc taatcgagtt cccttatcta tgttataagt ttctatagct ttaattaatc
      481 ctattgtacc tatggatatt aaatcatcct gatcttccgt tgaattatta tatttctttg
      541 ctatataagc tactaatctc atgtttcttt caattaatgt atcttttgca cttttatcgt
      601 tttctatttt aaatttagtt aaatactcaa tctcttcttc aggggttaag ggtttttcaa
      661 aagatttaag agctgccaat aggtcacccc cttttcaacg gtcactttaa attatatgaa
      721 ggagacttat ccaagttgac tgttatctaa taaaataata tgcatatttt tagaatttaa
      781 aacatatatt tgcaaacttt tgaatggaat atgacataaa gagtgggaat ttaaatttaa
      841 gtttttgttg tatatatatg aaacaaggcg aagaaattat tgatttataa agtataaatt
      901 ggaattgagg tgtcaataaa tataaaagga aatagtattt atatatgttt tggtgttgag
      961 gtaaaagatt aatgcaaagc attagagtct ataaaaatat gaatatagga ggtgtaattt
     1021 tacaatactt aaaaaagtaa atgtaattaa ttgtttgtat aaagttgatt acaagcaaaa
     1081 agtatcagtc atagttaatt ctgttaagga tgaaggtggt gtgttaattt atatatgtat
     1141 gggaagaaat tttgattaca atgaaaaaac ttacaaaaga tatttatgaa aaaacttata
     1201 gttaaatgtt tatatataaa atattattta ggggttgttt ttattattga aaataagaaa
     1261 gagtggtttt atatcaatcg aatgtataat aagtattgct atattatatg tggctgttta
     1321 tttagtttct acatcattgt ataattgtta tagttttatc agtagaaata tatctgacag
     1381 agaaatgtta agtacagcaa aaaaatatat agaagatgag aagtatagaa tacaaaatag
     1441 taagtatgag ttaattgaag ataagataga aaaaaattac ataaatggat atgaaattaa
     1501 cagtagaata gagcaaattt tagattatta tcaatgctat gaaataaata tagagataaa
     1561 aaatgaattt aaaaaactga ggtttaatag ctatgttact agaaaataaa ggctctatat
     1621 taataataac attgataata ttttctatta taagtactgt gtgcattatg tgtataggtt
     1681 taatttatac acataataat atgtttgagt taggatacaa ggacattcaa ctaaaagaaa
     1741 tttctttaag tggaatagaa atatcagtaa gtaatgtact ttcttgtgtt gaggaagcta
     1801 ttgatgtatc taataatgaa gacgaattta gagaatattt cttaggaaat aatatgattg
     1861 aatgtataaa aaaaataaaa gatacatctt atagtagttt agaagaactt tctttaaaag
     1921 tagaaaataa tactatttat gacagaggag atttttttga atttgatata gaaagtaatg
     1981 ttagagaaaa agaattttta aaagcaataa aagtaagagt gaaaatcaaa aatccatgga
     2041 taggaattga cattgaagat tatatggata aatctaataa caataaaatt gatgacaaaa
     2101 cattcaataa ttataaagca tctgaaaata atgaaattct aaatcaattt aaaaatgaaa
     2161 gtgaaaatat aaggaatcaa gaggttgttg aatttacaga agaagatgaa gaatatgaag
     2221 aagaacttaa acaaaacata attgaaaata taaagaagaa ttttgatgaa aaaaaactag
     2281 tacaggtata taagtatagg ggtgtataac tttgaaaaaa gaaggaatgt gactgtaata
     2341 gaattaatag tatctatcag tataatactc ttgatagtta cattatcttt tccaaaagat
     2401 aatcttgaaa atcataaaat aaatttattt gcacgtcaac tttgttcaga tataaggtat
     2461 gttaggcgaa ttaatatgtt tagcaactac aatacatata tatattatat aaatcaaggt
     2521 aaacacgaag gttatgtatt gagaaaagat ggtaaaaaca ttaaatcaat aatgctacct
     2581 aaaaatgcaa aaatagctag aagtattgaa ataataaagt ttaaatctga tgggtcgcct
     2641 caaaaggcgt ctacaataga gatatataat aatagattaa gaaaaactat aacaataaca
     2701 cctgtaagtg gaagagtact tctgaaagag ggaaaatatg aaacataaat atggttactt
     2761 gcttttggaa agtgtagtat cattgtcttc aatggtgata attatactag ttttatattc
     2821 tatatttttg tctacaataa acttaaagtt aaaagtagaa gataagatag aactacaaca
     2881 acaaagtctt gaaatcataa aatctatgga aggaattatt agcaattcta tgggaataat
     2941 gaatgtatct aattatgaag aaacatttaa aaagacaacg tctataaaat gtagatatgt
     3001 agatgaaaat aataatgaag aaagcataag taataaagaa attatattga atgaaagacg
     3061 aaataagtta tttgtaaatt ctttaaatgg cgaaagtagt caagcaggtg gttatgaaat
     3121 tggagattat gtagatgaga tgtatgtatt aattactaat aatggtcaat atgttaatat
     3181 taaattgaaa ttatcaaaac gtagtcaaaa atatgagact gatttcaaaa taaaagtatg
     3241 gaattttagt gaaagtatat aaaaatacat aaatttaaga taaagaaatg acatcaataa
     3301 gtcatataaa atataattat tgagaactat ataactaaat aatatcaata tgtaaagtgt
     3361 atagattaag aatatagagg ataataataa aaatatagaa taagtaattt gccaaatact
     3421 tgtttatatt gtataactta tgatattatg atattagtaa aaatttgtaa tcctgacttg
     3481 acagaattca agtaagagat ataattttaa aggcatagta atgtaaaatt tactaaaaaa
     3541 taagaaattt attttaatat ggaggaacaa aaaatggtat cagcaggtga ttttagaaaa
     3601 ggtgttacat ttgaaaaaga tggacaacca tgtttagtag ttgattttca acacgttaag
     3661 ccaggtaaag gagctgcttt cgttagaact aaatacaaaa acttaaaaac aggagctata
     3721 agagaagaaa gtttcaatcc aagtgaaaaa ttccctaagg ctgttataga tacaagacaa
     3781 atgcaatatc tatacaatga tggtgagtta tattatttta tggatcaaga aaattttgaa
     3841 caaataccat taaactatga gcaagttgaa gatgcaatta agttcttaaa agaaaatgaa
     3901 gttgctacaa taagatttta ccaaggacaa ccattccaag tggaagcgcc aaactttgca
     3961 gagctagaag ttacagatac agagccaggt ataaaaggtg atacagcaag taatgtaact
     4021 aaagcagcta cagttgaaac tggagcagtt gttcaagttc cattatttat aaatactgga
     4081 gataaagtaa agatagatac tagaacaggt gaatatttat caagagtata aaaatttatg
     4141 tatataaaag cttatatgtc ttaataatat aagatatatg agcttttagt ttatatataa
     4201 aaaagaaagc gaggggtgtt acatgaaaca tttatatgaa gaagttgcat acttaaaagg
     4261 cctagcagaa ggactagaaa taagtgctga aagtaaagaa gggaaaatga tacataaaat
     4321 agttgatgca ttagaagtat ttgcagatgc tattgtaaca ttggatgaag aacaagaaga
     4381 acttcaagat tttgttgaat ctatagatga agatttagca gatttagaag aagatattgc
     4441 tgacatggaa gaagatcttt atgaagaaga tgacgatgag gatgatgagg acttcagcta
     4501 tatagaaatg gaatgcccaa attgtggaga gttagtagaa atagacgaag atttgttata
     4561 tgatgatgaa gtagatgttg tttgtcctga ttgtaaggct gtgatattat catcagaaga
     4621 tgattgtgat gatgattgta catgtggtgg ttgtccaagt tgtgatgata gagagtaaaa
     4681 attcataaga attaaaactg cctatgatgc ctatgagata ttttgcttca taggtagttt
     4741 tatttttatg aggaagggta ttatgtattt tggttttgca atacttggac aaaaatagta
     4801 cactagaatt tatagaatag gattttggag ggatgtaaat atgaaaataa gtaaaaaatt
     4861 actagataat atacaaagat ttgaaaatgt tataaattat aaatttaaaa ataaagaata
     4921 tatactagaa gctcttactc atagttcata ttctaatgaa aataaaaaat ataattttaa
     4981 cgaaaggttg gaatttttag gggattcggt tttaggcata gtgataagtg attatttatt
     5041 taatgaagaa gctaatctgc ccgaagggga actaactaaa cttagagcaa atatagtttg
     5101 tgaagattcg ttaagtgaag tagctaatga tataaattta ggtattcata tgttattagg
     5161 tagaggagaa gaagcaacag gaggaagaca taggacttca atattagcag acgcatttga
     5221 agctgtaata gctgctatat atcttgatgg aggttttgaa agtgcaagac agtttatact
     5281 tcatcatatg gaaaatataa tttatgattc aagaaaagga aatatattta gagactacaa
     5341 aacgcatctt caagaagtgt tacaagggaa tggagaaaat aatatctggt acaggcttat
     5401 agaagaaaaa ggaccagacc ataataaaag atttgtaatg gaagttggaa ttaatgacga
     5461 tgtattgggt ataggagaag gaaagagtaa aaaagaagct gaacagttag ctgctaaaat
     5521 agctttaaag aaaaaattat gggaaaaata aatataatat gtaaaaatat aaaaagaaat
     5581 atttatatta gaaggatgtt gtagatgaaa aagagaatta tacctatatt tgttcctcac
     5641 aaaggatgtc ctcatgattg catattttgt aatcaaaaaa aaataacagg tgtgagtact
     5701 gatgttacta gtgaagatgc aagaaatata attgaggaat gtttagagac tatagataaa
     5761 gatgcagatg tagaaatagc attttttggt ggtagcttta ctgctataga tattgatata
     5821 caaaaaagtt tattatcagt tgctaaagaa tatgtagaaa aaggtcttgt aaaagatatt
     5881 agaatgtcta caagaccaga ttgtattgat gaagatatat taagtatgct aaaagaatat
     5941 aagacaagta taattgaact aggagtacaa tccttagatg aaaaagtatt attagatagt
     6001 ataagaggtc atcaatctga aattgtatac aagagttcaa agatgataaa aaatagtgga
     6061 atcaaacttg gattacagat gatgattggt cttccagctg acacagaaga aaagtgtata
     6121 tttacagcaa aaaaatttgt agagctaaaa ccagattgtg ttagagtcta tccaactttg
     6181 gtagtaaaag atacaggtct tgaaaagtta atggaacaaa ataaatataa tccatttact
     6241 cttgaagaga gtgtaaatat agtaaaaaaa gttcttgtac tgttttatgt taataatatc
     6301 aatgtaataa gagttgggct tcaagctact gatgatatac aaattggaaa agctgtttta
     6361 gcaggaccat atcatcctgc atttagagag ttggtagaag cagatatgat aaaagattat
     6421 ttagaatttg ttattttgca aaataaaaat ataaagcaaa tgttggtcaa atctaataaa
     6481 aagaatattt ctaaaatcat aggaaataaa aaaacaaatg ttaaatatat ggaagaaaaa
     6541 tttggtgtgc ttttaaaaac acaagaatcc gatttagata taaatcaatt agaaattgtg
     6601 ttagatggga aaagtttaat tagtgctaat atgagagaca ttcatagaaa attgtatgac
     6661 atttatgacc tttagtttta ataatgaagg gagagaaaag atttgtattt aaaaagacta
     6721 gagttaaagg gatttaaatc ctttccagta aaaacagata ttatttttaa agaaggaata
     6781 acagccatag taggaccaaa tgggagtgga aaaagtaata tatctgatgc tgtaagatgg
     6841 gtattaggag aacagagtat taagagtctt agaggtgata aactcgaaga tgtaatattt
     6901 gcaggtacag atacaaaaaa acctatgaat tattgtgaag tggctcttac tattgataat
     6961 tctgaaaatc aattggaatt agattttaca gaagtaacta taagaagaag ggcttataga
     7021 aatggtgaaa gtgaattttt tctaaataat aaaagttgta gattaaaaga tataaaagaa
     7081 gtctttttag atactggtat tggaaaagat ggttattcaa tcatagaaca aggaaaagta
     7141 gatgaaatat taagtaataa tcctttaagc agaagaaaag tttttgatga agcttgtggc
     7201 atatcaaaat atagatataa aaaacaggaa gcagaaagga atttaagtaa tacaaaggaa
     7261 aatttagaaa gaatagatga tgtatatata gaaattgaaa atcaattaaa gcctcttttt
     7321 aatcaacaga caaaggcaaa aaagtacttg gaaataagtg aaaaattaaa gatacttgaa
     7381 gtaaatagct ttataagaga aattgaagga atagaaaaag aattaagtga ggttaatgaa
     7441 catcgtaagg ttattgaaaa ggagctcaat gaaaaagaag aacaaaaaaa tgttgtagag
     7501 aaaaagcaag aagatattaa taaagaggta gaggtattac aagatgtaat tgaaaaatct
     7561 gtagattata taaattcaat taaaggagtt atatcaaaaa aagagtccca aataaattta
     7621 attaaggaaa gaataagaaa ttttacgaat gaaattagta gaaaaaattt agaaataaat
     7681 gatataaaag agaagctaaa tgaaaataag caatacataa aagaattaga aagtaataaa
     7741 ttatcaggta gtgaagaact atctaccttg caggaaaata taaaagtatt ggaggcaagt
     7801 aaagataaac aaaaaattaa gttagaatcc ctaaataatg agatagaatt attgaaagaa
     7861 agtataattg atatattaaa taagaaacaa gagtttagta ataagctgtc cactttaaac
     7921 gcaaataaag aaaatatgaa tatcagagat gaaaatataa attcagagat aacagagtta
     7981 aacaaaaata ttgaaataaa atcatctgaa ttagatacta taaataaaga atttaatatg
     8041 caaaatgaaa atttaaagaa tgtaaataat agacataaag aattatcaat taatttgcag
     8101 gattcaatta gtgaacacaa taaactagaa gatgaaatac agaaaagtaa atataattta
     8161 aatggatata attcaaagtt aaatgtctat atagatatgg aaaatcatta tgaaggattt
     8221 aatagaggag taaaagaagt actaaaaaat aaaaatttaa aaggagttca tggagcatta
     8281 ggacaaatta taaatgtacc tgaaaaatat gagaaatcta tagaagcagc tcttggtgct
     8341 tatatgcaaa atataataac tgataatgag tttagtgcta agtctgcaat aaactatcta
     8401 aaacagaata atttaggtag agtaacattt cttccattaa atataattaa atcaaataaa
     8461 gtaagtctag gaaatttaaa agcaaatact aagttcatag gaatagcaag tgatttaatt
     8521 acatttgatg aaaagtatag aaatataatt gaaaatatac ttggaagaac aatacttata
     8581 aataatatag atgagggcat aaaatttgca aaagaaactg gtcataggtt taaaatagta
     8641 actttagatg gtgaaatctt aaatccagga ggttctttga ctgggggaag tctaaaaact
     8701 aatgggaata tactatcgag aaaaagatac attaatgaat atacagaaaa gataagtaat
     8761 atcaagaatg aaatttcaca tctagaatta aaaagagaat ctttagacaa agatgtgaaa
     8821 aatatcaaaa atgaaattga ttcacatgaa agtaaaataa aagatttaga aaaaagtata
     8881 ataataaaaa gtacaagtat aaagaatgtt gaatctgaaa ttgaatcgtt aaaaggtagt
     8941 attactaaat tagaaaatga gaagaatgat ttaaattcta atctaaatta tacacttgaa
     9001 aaaagcgatg atgttagaaa agatatggaa gagttagatg atttatacaa taaaaataaa
     9061 gaaaaaattg atgcattgaa tgaggaaata aaaagataca atgatttata tgataaagaa
     9121 aaatctgaat ttgatgaatt gaacttaagt ttagtgaaga aaacagaagc ttataatagt
     9181 atagttagag atataaaaag aatatctggt gaaaattgtg agctagaaga aaagaataaa
     9241 caattagaag agtcattaaa ttatgaagaa catgaaataa ttaaattaca agattctata
     9301 gccactgaag aaaaagaaaa agaaaattta acaaaacaac taggggatag taatagaaat
     9361 cttgaaacta gaaaaatagc aaaagatgat ttaaaaaata gttttgatga aattaataaa
     9421 gaattaaaaa ctatagatag acagcatata gagcttaaag aaagtttatt taaagttggt
     9481 ggaagattag aaagattaaa aactagtcaa gacacatata taaataagtt gtttgagcaa
     9541 tatgatatga ctttagtaca agcattggaa attaaagatg aagatttgga tatagataga
     9601 aaattcttag aaagtctaaa gagagaaata agaagtcttg gaaatataaa tataggctct
     9661 ataaaggaat atgaggaaat aaaagaaaga tacgattttt atagcgaaca aaagcaagat
     9721 ttagaagagt ctatggaaga gatagaaaaa cttatacaca ctttagaaga aaatatgaaa
     9781 tctgaatttg aaattaagtt tgaagaaata agtaaaaatt ttaaatatgt atataagaga
     9841 ttatttggtg gaggttgtgg agaattaact attttagata aagaaaattt attagaaagt
     9901 gatatactaa taacagctca acctccaggg aaaaaaatga aaaatttaaa tctattatct
     9961 ggtggagaga aagctttaac agcaataagt atattatttg ctatattgat tacaaagcca
    10021 acaccatttt gtatattaga tgagattgaa gcgccacttg atgatgcaaa tatatttaga
    10081 tttggagaat ttttgaagga tttatctaaa gagactcaat ttatatcagt aactcatagg
    10141 agaggaacta tggaagctgc tgattatatc tatggagtta caatgcaaga aaaagccatt
    10201 tcaaaagtta ttagcttaaa attaaaagaa gctcaggaaa taacagatat tatataactt
    10261 agattgacat aggaggaata acagtgttaa aaaaattatt tggttttgga aaagacaagg
    10321 aaaaagaaat agaaaaaaaa gatgctgaag aagaaataga agttgaagac agtgttgata
    10381 gtttagaaaa cttggaagaa accatttttt caggacttga agaagaagtg atagataaag
    10441 tggaagatat tgaagaaaaa actgaggaaa gtgtcaataa tgaaggcact gaagaaatcg
    10501 aaaattttga aaaaatagaa gttgagatgg atagtaatgc aaaagaagta gaaaatattt
    10561 caaataatga ttctcaagaa gagataatcg aagaattaga aaattatgat gaaattgagt
    10621 ctaaagagat agaagataaa gaagataaga aagttaactt gtttgaaaga ctaaaacaag
    10681 gattgacaaa agcaaagcaa ggaataactg atagaataga tgaagtttta aaatcatata
    10741 caaaaataga tgaagaatta cttgaagatt tagaggaaat tttaataaca gcagatgttg
    10801 gtgtaaatac aactatggat ataattgaaa gactaagaga taaaataaag caaaaaggta
    10861 ttactgaacc aataaaggta agagaagaac ttaaatctat agttgaagat attcttacaa
    10921 atgaaaactc tacactagat atagaaccag caccatgtat aatattaatg gtgggtgtaa
    10981 atggggtcgg taagactact acaataggta aactagcaaa tagatacaaa aaagatggta
    11041 aaaaagtttt attagcagca gcagatacat ttagagcagc agcaacagag caattagaaa
    11101 tttgggcaaa tagaaccaat gtagacataa taaaacatca agagggagca gaccctggag
    11161 ctgtagtatt tgatgctata aaagctgcaa aagctagaaa aacagatgta ttaatatgtg
    11221 atacagcagg aagattacac aataaggcta atcttatgaa tgaacttgga aaagtattta
    11281 aaatagtaga tagagaattt ccagaagcaa aacgtgaagt acttcttgtg gtggatgcta
    11341 caacaggtca aaatgcagtt gttcaagcaa agacatttaa agaagttgca gatataacag
    11401 gtatagtact tacaaaactt gatggaactg caaaaggtgg agtagtcctt gcagtaaaat
    11461 cagaagtaga tgtacctgta aaacttattg gagttggaga aagtgtagaa gacctacaag
    11521 atttcaatgc aaaatcattt tcagatgcat tatttggtaa ctaatgttct tttaaaaata
    11581 atggttgaca agagtgttgt tatgatataa aatacaatag tgtaaagtaa ataaccttta
    11641 cagtatactg ctaggaagtg taagatatga atatagaaaa aatggtcgaa attggattgt
    11701 tatttgaaca gtataaagaa ttattaactg acaagcagaa agaaatagtt gctctatact
    11761 atgaagaaga ttattcttta ggtgaaatta gtgaaaatct gaatgtatca agacaaggtg
    11821 tttatgatac cttaaagcgt tcagaaaaaa tactaagaga ttatgaagaa aagttacact
    11881 tagtatcaaa gattcaagag caggaaaaaa atattaagat tataaaagat aaaattattg
    11941 acattaaaga agatttattg cacaatagag attgtgctaa tttaatccct aagctagaaa
    12001 atatagaaga tgtatgtagg gagatgataa aatgatattt gaaggattgt ctgataaact
    12061 acaaggggca tttagtaagt taaaatctaa aggaaaactt acagaagtag atgtaaaaaa
    12121 tgctatgaga gaagttaaac ttgctcttct tgaggcagat gttaacttta aagtagttaa
    12181 agatttcata aaaaaagttc aagagagatg tgttggacaa gaagttatgc aaagcttaac
    12241 tccagctcag catgtaatca aaattgtaaa tgaagagctt acaagtttaa tgggagatgt
    12301 acaaagtaaa gtaatgattt cctcaaagcc tcctacaatt ttaatgatgg ttggattgca
    12361 aggtgcaggt aagactacta cagcaggaaa acttggagga tattttaaaa agcaaggtaa
    12421 aaaacctcta ttagtagctt gtgatattta tagaccggca gctataaagc aacttcaagt
    12481 tgttggagaa aaattggata tacctgtatt caatatggga gataaagaaa atccagtaaa
    12541 tattgcgaaa gcaggtttaa gccatgcgat taaaaatgct aatgatttag ttattataga
    12601 tacagctggt agacttcata tagatgaagt acttatggct gagttaaagt ctattaaatc
    12661 agaagttaag ccacatgaga tacttttggt tgtagattct atgacaggtc aagatgctgt
    12721 aaatgttgca gaaagcttca atgaggcact tggtgttgat ggagtagtat taactaaatt
    12781 ggatggagat actagaggtg gtgctgcact ttcaataaga gcagttacac aaaagcctat
    12841 aaaatttatg gggatgggag aaaagctaga tgatatagaa cctttccacc cagatagaat
    12901 ggcatcaaga atattaggta tgggagatgt cttaagttta atagaaaaag cacaagaaaa
    12961 tatagactta gagaaagcta aagaattaga gcaaaaaatt aaaaaacaag agcttgattt
    13021 tgaagatttc ttacaacaaa tggaacaaat acaaaatatg ggacctcttg acaagatact
    13081 tggtatgata cctggtatgg gaaatgtaaa agaccaactt ggagatgtag atttgaatgg
    13141 caaagaaatg aagagaacaa aagccataat tcagtcaatg actacatatg agagaagaaa
    13201 cccaggagta ttaaatgctt caagaaagaa aagaatagct aagggcagtg gaactagtgt
    13261 acaagatgta aatagactta taaaacaact taatgaaatg aaaaaaatga tgaagatgtt
    13321 tgctggttca caaaagagta tgaagaaaag gggcggctta ggcggtttac ctttttttaa
    13381 ataaaatata aaaaagtaaa aattattcgg aggtgactgt aatggcagtt aaaataagat
    13441 taaaaagaat gggagctaac aaaaaacctt tctacagaat agtagtagct gattcaagag
    13501 ctccaagaga tggaaaattt atagaagaaa taggatacta taatccaatt tctgaaccta
    13561 aacaagtaag aataaatgat gaaaaagcta taaaatggtt agctactggt gctcagccaa
    13621 cagaagtagt taaaaaatta cttgtaaaaa acggagtaat agaaaaattc gaagcttcta
    13681 aacaagcaaa ataatttttt cttggcaatt agtttaagta agggctcatt gctaaggtta
    13741 ctttaggaga tgaaatatat gaaagagcta gttgtagata tagctaaggc tctagttgat
    13801 aatcctgact cagttgttgt tgaggaattt gaagataatg atggtatcgt cttaaagctt
    13861 acagttgctc aagaggatat gggtaaggtt attgggaagc agggaagaat agctaaagct
    13921 ataagaactg tcgtaagatc tgttgcaaat agagaaaata taaaagtttc tcttgagata
    13981 gtataaaata ggaggattag gtataatgcc tagtcctttt tatataaatg cgtagtttaa
    14041 gattttatga tgatatgacc acataagatt aaatttttta agaaggtgat aacatgaaag
    14101 aaaaattaac tcattttaag gtaggtcaaa tagtaaatac acaaggttta aaaggtgaag
    14161 taagagttta tccacttact gatgatatag agaggtttga tgaattaaaa gatttctatc
    14221 tgggaaaaga tttaaataat aaattggctg ttgaaaaagt aagatacaaa ggttctatgg
    14281 ttataatgaa gataaaaggc atagacagca tagagaaagc agaaaaacta aaaaataaat
    14341 ttatgtatgt atctagagaa gatagcagag agcttgaaga aggcgaattt tttatagcag
    14401 atatgattgg aatggaagtt ttaactgtag atggaaaata tgtaggaata ctagaagatg
    14461 ttcttcaata ctcagcaaat gatgtttatg tagtaaaagg tgaagaagat aaagaattta
    14521 tgataccagc tataaaaaaa tttgtaccta caatagatat tgatgaaaga aaaatgatta
    14581 tagaccctat taagggaatg atagattagg tggtaatatg agatttcata taatgacact
    14641 ttttccagaa atatttaatt cgtacatgga tgaaagtata atgaagagag cagttgaaaa
    14701 aggtataatt gaggtttata tatataatat aagggatttt tctaataata aacataaaaa
    14761 agtagatgat tatccatttg gaggaggagt ggggatggta atgactcctc aacctatata
    14821 tgacacatat aagcatataa taacaactca caatataagt aatccaagtg taatttatct
    14881 tactccaaaa ggtaaggtat acaatcaatc tatagcaaag cagatgtctt taaaagaaga
    14941 tataatacta ttatgtggcc attatgaagg agtagatgag agaatcattg atttaatagt
    15001 tactgatgaa atttcaattg gagattatgt attaactggg ggagagctcc cagcacttat
    15061 aatgatagat tctatatcaa gattaatacc aggtgtatta aaccaagaag aatcatttga
    15121 agaagaatca tttaaagata acttattgga atatcctcat tacactagac ctagagattt
    15181 tgaaggatta aaagtaccag aagtgctttt atcagggaat cataaaaaga tagatgagtg
    15241 gagaagagaa gaatctataa ggataaccaa agaaagacga tttgatttat ataaaaagtc
    15301 taatgaaaaa taaaaatata atgaaaaatt aatatatatg tatacaataa agtacctata
    15361 aatggaatta aaaacataaa agtgcttgta ataactattg aagtatagta taatataaaa
    15421 tgttgaaaat acgggcgttc ctctgtttac actggtcggg tagttaagaa cgtctatgat
    15481 atctaggagg aagaaagatg aatgaaatgc taagagcaat tgagcaagaa caattaaaaa
    15541 atgaagttcc taattttgga cctggggaca cagttaaagt acacgttaga attatagaag
    15601 gaaaaagaga aagaatacaa gtattcgaag gtgtagtatt aaagagacaa ggtggaggag
    15661 caagagaaac tttcacagtt agaaaaatgt ctttcaatgt tggtgtagaa agaactttcc
    15721 cagttcactc tccaaaaata gaaaaaatag aagttactag aaaaggtaaa gtaagaagag
    15781 ctaaactaaa ctacttaaga ggtagagtag gtaaagctgc taagataaaa gaagctagaa
    15841 ataaataatt aaaaacttta cttataaatg gggggctgat ttatcagtcc tctttctatt
    15901 ttaatgaaaa ttacagcaaa taattatact agaaataaat agaaatattt gttgaaaata
    15961 tacattattt atctatgaat ttatagcttt taaaatgaaa aataaaattg gtaaatattg
    16021 gattatattt tgtaacgata ttaaaaataa gagaacagta ttatgtaaag tatgacgtta
    16081 aaaagatgac aatattatag tttgtgaaga tagatttttt aagtggaatt agaatataat
    16141 aatataatat taataaggag tgatacatgt gtcaaatgaa tacgaagagt atcttatgga
    16201 tgacaatcta catataaact ggtatccagg tcatatgaaa aaaactaagg agttagtaaa
    16261 aaataactta aaattgattg atgtagtaat tgaacttttg gatgctagaa tcccgtttag
    16321 tagtaaaaat ccagacatag acaggcttgt aggagataag ccaagagttg tcgtacttaa
    16381 taagagtgat atggcagata gggataaatt gaatcaatgg attgaatact ataagaaaat
    16441 taatatcaaa gctataccag tagatacaat aaaaggtgtt ggtataaata atataatcga
    16501 agaatgtaaa aatgtaacta gagaaaaaat gagttcttta aaagacaaag ggagaaaaga
    16561 aagagctata cgaataatga tagttggagt tccaaacgtt ggtaaatcgt ctcttataaa
    16621 caaattaaca ggtagaaaaa gcactcaaac tggagataag ccaggagtaa cgaaagggaa
    16681 acaatgggtt agattaaagg gaaatcttga acttttagat acacctggaa tactttggcc
    16741 taagtttgaa gaccaagaag ttgcattaaa tttagcattt agtagagcta taaaggatga
    16801 gattttagat actgagactt tggctttaag acttatagaa aaacttatga aaatagaacc
    16861 tgagaagttg aaagccagat ataagttaga ttgtctagga gaaactccaa ttgaaactat
    16921 ggatatgatt ggccataaaa gagggtttat aacaggtaaa aaagaactag attatacacg
    16981 catagccgca actgtattaa atgaatttag agatggaaaa ataggaaata taacattgga
    17041 agttccagaa aacgttaaaa gatagatttt aaaacaaaat caaagattta atgttaaaaa
    17101 aataaaaaag ccttttagca aaggct
//
LOCUS       DACTGW010000067        17121 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.067, whole genome shotgun sequence.
ACCESSION   DACTGW010000067 DACTGW010000000
VERSION     DACTGW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.067"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(204..728)
                     /locus_tag="I9573_001741"
     CDS             complement(204..728)
                     /locus_tag="I9573_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAT4796365.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(793..1545)
                     /locus_tag="I9573_001742"
     CDS             complement(793..1545)
                     /locus_tag="I9573_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HAT4796366.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDISKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1576..2874)
                     /locus_tag="I9573_001743"
     CDS             complement(1576..2874)
                     /locus_tag="I9573_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HAT4796367.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQQGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREVNVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2891..5155)
                     /locus_tag="I9573_001744"
     CDS             complement(2891..5155)
                     /locus_tag="I9573_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HAT4796368.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5410..6228)
                     /locus_tag="I9573_001745"
     CDS             complement(5410..6228)
                     /locus_tag="I9573_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAT4796369.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKIIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIIL
                     LDEPTAGLDPVSTRSIVDIIKGLNKDNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFVNENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6221..6919)
                     /gene="cbiQ"
                     /locus_tag="I9573_001746"
     CDS             complement(6221..6919)
                     /gene="cbiQ"
                     /locus_tag="I9573_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HAT4796370.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIKNIFVLSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKMHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6922..7206)
                     /locus_tag="I9573_001747"
     CDS             complement(6922..7206)
                     /locus_tag="I9573_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HAT4796371.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPDYEPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGVQKGKRS"
     gene            complement(7196..7948)
                     /locus_tag="I9573_001748"
     CDS             complement(7196..7948)
                     /locus_tag="I9573_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HAT4796372.1"
                     /translation="MKQNIKLGVIAALMLIVLTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIISFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTVTSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8137..8329)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8461..10233)
                     /locus_tag="I9573_001749"
     CDS             complement(8461..10233)
                     /locus_tag="I9573_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HAT4796373.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYNMDKHPALDSMTRRIVNDIRNIDTN
                     QDMMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10247..11176)
                     /locus_tag="I9573_001750"
     CDS             complement(10247..11176)
                     /locus_tag="I9573_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4796374.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11405..11593
                     /locus_tag="I9573_001751"
     CDS             11405..11593
                     /locus_tag="I9573_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4796375.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRAKRRKEVQYE"
     gene            11586..12287
                     /locus_tag="I9573_001752"
     CDS             11586..12287
                     /locus_tag="I9573_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004069670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4796376.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLLDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSINNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12289..13212
                     /locus_tag="I9573_001753"
     CDS             12289..13212
                     /locus_tag="I9573_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4796377.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13369..14292
                     /locus_tag="I9573_001754"
     CDS             13369..14292
                     /locus_tag="I9573_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796378.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14285..15022
                     /locus_tag="I9573_001755"
     CDS             14285..15022
                     /locus_tag="I9573_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4796379.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            15033..15806
                     /locus_tag="I9573_001756"
     CDS             15033..15806
                     /locus_tag="I9573_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4796380.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16347..16955)
                     /locus_tag="I9573_001757"
     CDS             complement(16347..16955)
                     /locus_tag="I9573_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAT4796381.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5510 a         2979 c         2150 g         6482 t
ORIGIN      
        1 ataaacaatc ataataaaat ttttttaata aaactttatt ttttagttaa aatcatacaa
       61 tatatagctt tccacaatac aaataaatta aaaagtgaaa tgtatttgtc taaaaacata
      121 ataacatttc actttatcct catttttaag taatatatat ttttaatttt tatttataac
      181 tcaatctagt ttttatataa gttttagttt ttttctacat ctgagtaatc tattccaaaa
      241 gtttctacag taacagactt cataatttgt ggagtttgtg gcttatcagc tgaatctgtt
      301 ctaactgatg ctatttcatc aactgtatcc ataccttcta tcactctacc aaaacctgca
      361 tactgaccat ctaagtgtgg tgagttttta tgcattatga aaaattgaga accagctgaa
      421 ttaggtgcca tagttctagc cattgatatg actcctcttt catgttttaa attgttagta
      481 aatccattac cactaaattc accttttatt gagtgacctg ggccacccat tcctgtacca
      541 tttgggcatc ctccttgtat cataaatcct ggtattactc tatgaaatat tattccattg
      601 taatatcctt catttactaa tgatacaaag tttttaactg tatttggagc tatatttgga
      661 tataactcaa ttttgatttc ttttccattt tccatttcta tagttactat aggattttta
      721 ttttccataa tttattacct tccttttcat tagttaaatt acataattac atttattata
      781 ttatactcta aattaaagtc tatcaagtat attttttcct atttcgtatc cttcttgata
      841 aatagtttta caaaactcat catcaaagtt aagcgtatct ttaggagcaa attgagtatt
      901 aggtctaact actattattt tatcttcatc atatactttt ttaatatcct ctggcaactc
      961 ataattatat tttgtggata ttaaaataat tttatccaca ttatccccaa ttaatggttc
     1021 aattaatgca ctcctaatta aacctccatc taatttgaaa ccatcataaa ttccttcttg
     1081 cacatctttc acaaattgtt ctttaaatcc aagagttgcg tttggatttg atggtaacaa
     1141 cccactgtat gaaatgctga taatggcttc attacgaggc atttttgata tgtcaacaat
     1201 cttttctgat actactttat tatcaacttc aacataatta acataaaagt tcatttcttc
     1261 tatattacta tcatctagtt cccttaggtt gtctatagca ggagaattat ccacagtatt
     1321 atttactatt tttacatttc cattctggat atcttttata tttgtccaca ttttctccaa
     1381 gttatcaaca ttccctgtgt acaaataata tccatttatt gctcctattg atgtccctga
     1441 aatcgaataa aatttattta ttcctccatc atacaaggct ttaactactc ctgcttgata
     1501 agcccctttt gctcctccac cttctaaaca taatcctaat ttcatagtct catctccaaa
     1561 ttgtttatct tatatttata tttcaaaaaa cttactaaat gttttatata tactatagtg
     1621 gtcctcaaca tttcctaatt ctcttataga gtgcattgct aaaattggac ttcccacatc
     1681 cacagatggt atatctatat gagtagaaga tattggacct atagtacttc ctcctctttc
     1741 atctgaccta ttaacaaatt cttgatactc aacattaact tctctacata tatttttata
     1801 aatagctata gaatatccat cactagtata tgcctgacta gcatttatct ttataactgg
     1861 accttttccc attattggtt tattagttgg gtcatgcttt tctggaatgt ttggatgaat
     1921 agcatgagct aaatctgatg atatcataaa agatgaataa atagactcaa agaatccctc
     1981 tctatcctta cccattgata tgcaaattct ctccaatata tttaacaaca tgtttgaatc
     2041 tgcaccttgt ttagtagagc taccaacttc ttcattgtca aatacagcca ttacatttac
     2101 accttgttga cctttagtat taacaagagc attcaaacta gcatgtacca ttgcaagatt
     2161 atcttgtctc ccaatagata taaattcatc attagcccct attaaacttc ctttttcaaa
     2221 ttcatataaa aataaatcaa aatctataat atcttccata tttacatcta agttagatgc
     2281 tatttgcttt ataagaaaat tatctttttc taatgtatca tttaacatcc caactaaagg
     2341 taacatatct ttttgcttat tatatgaatg accatcattt atacttctat ttaaatgtat
     2401 tgctagattt ggtattatac aaattggctt tttaaagtct ataagccttt catttggttt
     2461 taatatacta tcacctttta aaactactct accagctatc ccaagaggtc tatccaacca
     2521 tgtacttaat attgctcctc catagcattc tgtatttaac ttcaaataag atttttcagc
     2581 ttctatttct gcatttggtt taatactaaa tgtaggagaa tctgaatgag agcctattat
     2641 tctaaatcca tctctttcta tatcttctga atttactaca aaagctacta tagctgatag
     2701 attcttagta acataatact ttccaccaac tcttagattc catctttgat tcaaaactaa
     2761 ttcttcaaat ccatttttat ctaagatttc cttagtactc tcgactgcat gaaaagacga
     2821 cggactatca tatatgaagt ctattaagtt tttagcaaag tttatactat ccattcctca
     2881 tttacctcca ttataaaata tttagtgttg tatattcttt atttattttt ttaattccct
     2941 gttggtcaaa agctatagta acatctgtac caattataga aaccactgtc ccaaccccaa
     3001 atttaggatg atgtacctta gaaccaagtt ttatatcatc tggattagtt tctttattag
     3061 aatcttttat tgtagcatta actttatctg caactttaga tttgcttata gtactcatat
     3121 actttttagt atatttatct aacatattat aacttgcttt agaatagctt agttcttttt
     3181 ttactttgta caatctttct atacattctt ctggcagttc ttccataaat cttgaagcta
     3241 ttgcaacatt tgttttacca tataatgttc tcttctctgt caatgacata tatagctctt
     3301 ctttagctct agttatacca acataacaaa gtcttctctc ttcttctatt tgtgaatcac
     3361 tcatagattt tatagctctt gatataggaa ataacccttc ttccatacct gttaaaaaca
     3421 caacagggaa ctctaaacct ttggatgtat gaattgtcat aagcgaaacc ctatcattgt
     3481 cttcttcatc atttgattca gatgtaagtg ctatacttgt taggaatgtc tctaagtctt
     3541 tattttcata agtatcatca ttactacttt caaactccaa agctatagaa ataaattctc
     3601 tcaaattatc aattctgtct tgagcttctt cacctttgcc tgttaaatct ttttcatttt
     3661 tatttcttat ctctacaagc tcgtccatat atcctgtagt atccaagact ttttctataa
     3721 gtttgctaac aggataaact tcttttatag ttctaagagt ccctataata tcaacaaatc
     3781 cacttatact tgcccttgcc ttagtagata tatctgaatt tgtttctata tcaattaata
     3841 ctgagtatat actttcctgt tttaagttag ctctatcttc aattttttct atagttctaa
     3901 gccctatacc tcttctagga acatttataa tcctttttat agatatatca tcttgtggat
     3961 tttgaataac tcttaaatac gccaacaaat cctttatttc ttttctctcg tagaattttg
     4021 taccaccata tatattgtat ggaatttgac ttctatttaa agcgtcctct acaggtcttg
     4081 cctgtgcatt tgctctataa agtactgcaa aatccttata aggtctattt tcttttctag
     4141 ctatttgtgc aattgaatca gcaatgaaat cagcctcttc tatttcgcta ccagttaatt
     4201 gtatctttat aagttctcct tctttttttt cactccaaag cttttttctt tttctttcta
     4261 tattattaga tataacttta ttagctgcat ctaatatcac ttgtgtagac ctataatttt
     4321 gttctaactt tacaacatgt acattgtcat aatctttctc aaattcaagt atgtttctta
     4381 tatctgcgcc tctccatcca taaatacttt ggtcatcatc tccaacaaca caaatatttt
     4441 gatgttctct tgctaataat tttatcaatt catattgagc tttacttgta tcttggtact
     4501 catctaccat tatgtatcta aatctacttc tgtaatatgc taatacttca tcattagcct
     4561 tgaaaagttc tacagtttta agtattaaat catcaaagtc aagtgcacta tttcttttta
     4621 aacggtcttg atataacgca taaatatctg caatcttaac cattctatta tctgccatgt
     4681 tgatatcttt aaactgcttt ggagtataaa gtttgtcttt tgctcctgaa attgcactta
     4741 taactgcttt tggttcaaac actttatcac ttaaatttaa ttcttttaga cagtctttaa
     4801 ccaatgtaac ttggtctgct gaatcataaa ttacaaaact tctattgtaa ccaatcttat
     4861 ttatatcttt tcttagtata cgaacacagc aagaatggaa tgtacttatc cacatatcct
     4921 tagtttcagg acctatattt tgttcaactc tctctctcat ttcatttgca gctttgtttg
     4981 taaatgttat agctaatata tttggtgctt gtacaccttt atcttctata agatatgcta
     5041 ttcttgttgt taatacctta gttttacctg aaccagcacc agccagtata agtactggac
     5101 cttcagtctt ttctactgct tctctttgag caggatttaa tgtatctaaa ttcatatttc
     5161 tcctcctgtt aacagccata aatatatttt aatctaaaat atgaattact tcaaattata
     5221 attatgtatt atattaatag attatttcca atatattaca attaaataac atttttccat
     5281 ataatttatt taattgttta ataaaaaaaa taaccttatg atttggggaa tcataaggca
     5341 cctttaaggg gtctaggtta agggttatta tatataaatc tattttttta aaataggttt
     5401 agggcttaat tatttatttg tattaagttc tctttccttc caatacttat acaaatcttc
     5461 ttctttctta aataagggta aattcatatt tctatgtact tttactaacc atggggattc
     5521 taggccagct tgtataatgt tattttcatt tacaaataca ttttctgcct ttccttcatc
     5581 aattaaaatc cccttatcca atacatatat ataatcgcat atttcataca ttaaattcat
     5641 atcatgactg gatattacta tttttatatt atctttattc aatcccttta ttatatctac
     5701 aatagaccta gtacttactg ggtctaaacc agctgtaggc tcatcaagca atataatttc
     5761 attttccatt gctataactg atgctatcgc tacccttttt ttctgaccat aacttaaaaa
     5821 atgtactggt ctatctataa aatctattcc attaacagct actagagctt tgtttattct
     5881 ctctttaatt attttttcat ctataccaat atttctcatt gcaaaggcta tatcatcata
     5941 tacttttgaa taaaaaattt gtttttctgg gtcttggaac acaataccta cattttttct
     6001 aaggttgtat aaacttctct tatcatattt taacttttct tctttaaaaa gaatttctcc
     6061 agatgtaggc tttaatatac ccattaaatt cataaataat gttgattttc ctgaaccatt
     6121 tgaacctatt atacctataa catttccctt tgaaaaatcc atattaatat ttaatagtgc
     6181 attggtattc ttttcatatt gataagttaa attatttatt ttaaacatca ttatccccta
     6241 ctatgtgaaa tttaccatca tacaatttta tgtctaatga tatacacata tcatcatacc
     6301 ttgtcataag tcttttgtac agcatactgc caagaattcc taatgattta tatgaagttt
     6361 ttaaatttat gtacccaaat cttagttctt gtgacttata tatttctgaa aattcttcta
     6421 aaaatatgaa tataaaccta tatattaaca ttgatagctc aacaaaagta tcaggcaaat
     6481 gcattttttt aaagaagaat attaattgat taaaaggtgt tgtaagtata caaaagtata
     6541 cacaagtcaa acaagacata gctctaaata acacatgaat agacatgtca atagatgctt
     6601 ttgatattcc tacattgaaa ctaaaaattt caaaactata taataagtct gccttattaa
     6661 aagatatatt tacaagagtt attcctatac taagaaataa aaagtacatt ggtattctga
     6721 gtaattttaa ataattcttt aaatctattc cagctacaca aacaactaga atactcatta
     6781 aaatcataat tccacttaaa acaaatatat tttttattac catagaagca atcagaaaga
     6841 ttactcctat ggctactttt ttatttggat ttactttact taatctattt gtatatgcat
     6901 atttatcaat tataagcata attaacttct ctttcctttt tgaacaccta atatgtaccc
     6961 tattacacca gcacctaacg ctgcttgtgc agagaataat aaactttcta tttctccact
     7021 tggtggctca tatgggctac taaaccatgg ttcataatct ggatttatct tagtaatttc
     7081 actctcagct tgaccatctg ctcctccata ttcagctccc gaattaacaa gtaatgggaa
     7141 tatgataaga gctactacta ataaaagtag taatatatta gttttcgtct ttgttttagc
     7201 actcattagc tttaactcct ttatcattat tttctagtat gcttttttct ccattttctg
     7261 taattaaatt atatacaatt acagtaagaa taccctctgc tattgctatt ggtatttgag
     7321 tcataaagaa aattcctaaa aacttaattg ctgaagccat cactccacct gatgggtctg
     7381 ggaaagccag tgctaactgt atcgaagtta ctgtatatgt agccaaatct cctattgctg
     7441 ctgcaagaaa tactggcatt gcattatttc cgtctttttt ctttaatgct ttaaatatta
     7501 agaatgatat aattggtcct ataatagcca ttgaaaaagc attagctcct aaagtagtta
     7561 ttcctccatg tgccaatagt aatgcttgga atattagaac tatagttcca agaacaaaca
     7621 ttacacttgg tccaaacatt attgctccaa gacctactcc agtaggatgt gaacaacttc
     7681 ctgttactga aggtatcttt agtgctgaca atacaaatac aaatgctcca cacagcgcta
     7741 ataatacttt ctttttaggg tcttgcttta ctatttttcc tattgatttt agtcctacta
     7801 aaaaaaatgg tataaatata actccccatg ctatagacca ttttactggt aagtatcctt
     7861 ccattatgtg cattgcattc gatgtaactg gagttaaaac gattaacata agtgcagcta
     7921 taacccctaa tttgatattt tgtttcattt tactatcccc tctctccctt tctaataatt
     7981 aaaatataaa taatatatgt ctgatttatt ttaaaaataa aaataaaaaa gcctaactcg
     8041 atggctaggc ttctaatttt cgataatcca aatacaaaca cataaaatga acatatctgt
     8101 ataattatta taagaaactt tctccatccc cgaagaaatt actttaatat tttaggcagg
     8161 tctcctgact tagcttcttc ctactcctct tccttcccat gagatataaa aaatatcatc
     8221 atagtggcta acaaaagttt cgtctgcatc acagtagcgg gggctgtaaa ggaatttaac
     8281 ctttttccct attaatcttg cttaaagaac ctataatatt atatatttaa tttttaaatt
     8341 attcttaaat cattttctat ataaatcatt gtataccttt tctgtgaaaa tttctacatt
     8401 ttttataaat tttattcatt tttatatttt tatgtatata aattcttttt tgtaagttta
     8461 ttaatttcta attatcttct ttaaatacat tgaaactaca tcagaaaatc tagttatatc
     8521 tcttgaatat ataaaatcat ttccatatat taatctttca gcttcaagtt ctgattcttt
     8581 tcctgtaaat actcctagta ctaatatatt ttgctttcta gcttttctca cttcattggc
     8641 tgtatctctc aaaccaacta tacccctata agccttttct cctcttaatg ttctctcact
     8701 actttttcct atctttacat catttggtct tccatcgctg agaactatca ggattttatt
     8761 ttcttcatct ctatttatta gaccactaca tatagatttt attgccaaac catctctatt
     8821 attaccagca caaaaatatt caaatatatt ttcactattt tttatactat catcataatc
     8881 tttaaatctt tttaatattg tatagtctaa aaaactagaa aatcccatca ctctatttgg
     8941 tatgccaact tctgttagag catttgcaat tatgtaagct tgaattgcca catttccctg
     9001 atttctactt tgagagccac ttgcatccaa taatatatca ataacgtatt tccctttttc
     9061 attttctata tctttataaa atattttatt attattactt ctaccaactc tccaagctct
     9121 atttgctaat atagtaccac aatcagaata tactctactt ttagcatttt cttctattaa
     9181 aattcttgac agactttctt ttaatctctt tatttgctgt ctatacattc tagcatgttc
     9241 tctaaatttt cctaaattat tttctttttg tcttgttaca tattttagtt gaaatacatt
     9301 attacactca cttctaagaa ctccatctgt aaagtgtatt ctacaacctt catgaacatt
     9361 tctacaattt tgtgtctcaa tttttttaat ttcaccttta ctcaaataag tttttccata
     9421 ataatgttct atttttgaat atatcttatt tgcagtgtct tcatcaacat atataactcg
     9481 gtctgctgaa ttgctacatt tagaatcatc aaaatcccct acaccttcta caagcatgga
     9541 tgaagatatt gaatctatat cactttcaat aatattttct tcttcatcac tataaagttc
     9601 ttcatacatg aaatcagcaa atgtatcaaa atttacttct acactttcta cattattttc
     9661 atcaataaga ctattttctt ctccatttaa tatgtattcg aaataagtta aatatataac
     9721 atctaatgct tttaaaatct ccatcatatc ttgattggta tctatatttc ttatatcatt
     9781 aactattctt ctagtcatac tatctaatgc tgggtgcttg tccatattat aaagtacaaa
     9841 tgtgtattta attttatcca taatattatc attgtgtatt ttggtaaaac gtgaaacaat
     9901 atctttataa gcatttcttc ttatgtcttc tatccctggc ctatctttaa tgaccttttc
     9961 ttcaactatt gtatttaata taacttgaat tagacccagt ataatatctt tgttgtatcc
    10021 ttttcttatt ctatttgtaa gatatttttt aactatatcc caatctacat actttcttct
    10081 agcacctgca attattgcaa aatatagacc tatttctttt gaagagtaat ctttttctga
    10141 tatatctata ctttcattgt aatcaccagc tattgtccat gataagttat tagctctatt
    10201 ttcaaattca taatcatcat atatccattt cataatctca cctatattaa atatgactta
    10261 ttggaaatac atctatactc tcccatttat taggtattct agttttaatt acatctccaa
    10321 ccatttcttt ctcatataca tcaaaagtct ttcctgtaag tcccatatta attgcaagag
    10381 ttggttttaa tcctcttctt atagtcttaa gagatgccat aagaccacgt aaatcaatag
    10441 ctttactaga tatctctcca ttttgagact tcaattgtaa atctaaaaat attcctgcaa
    10501 agtgtattaa tttttcttca tcagcatctg aaaattcatt ttttaaaatc atcattaact
    10561 tatcttcttc tataggagga atatctatta ccataaatct agataccagt gcttcattaa
    10621 gttcttttgt tcctgcatat tcataattca tagtcccaat aaatcttgtt gctggatgta
    10681 gattgactct ctcataccca ggtacatcaa ttattcttct atagtctaaa gctgaatgta
    10741 ccacaactat agcatcattt tttgccatat taatttcatc aaatactcca aaacctccat
    10801 taatagcaca ctcataaact gagcctcttc taagtttaac ttcattgtct ataaatgtat
    10861 ctgtacctat aagtgttgaa ctgtctgtat ttatatgaaa tgatgtattc cactgaggtc
    10921 tattaaatat ttcccctaga ttatcagcaa gtaaattttt tcccgttgcc tttggtcccg
    10981 ataataatat attttcttct tctaaaatag ctgctataca catagacaag atatctttac
    11041 cataaaaata agttttagac tttgttactc tgtattctaa atctttttct aaaccataat
    11101 aatttctaaa atacagtaca tcttcaataa gtttttcatc taccccttgc acccttagat
    11161 tatctattat atccatattt ttaccccttt tatataaata gtatttgttt catttcttat
    11221 caccatttta gcataaaagg atataagcta aaattgattt tttaatctcc cccattttaa
    11281 gtaaaaccat gcaccatttt atcactacaa aattaagagt actacttact gatatttctt
    11341 gattttgttc gtctatacgt atataatata tgctgataaa ttatgaggaa aggatgattg
    11401 aaatatggaa tatatgtctt gcccagaagt agcaaaaaaa tggggcattt ctgaaagaag
    11461 agtacaaaaa ctatgtgaag aaagtcgcat accaggagtt tcaaaactcg ggtatatgtg
    11521 gctgatacca aagaacgcag aaaagcctgt tgatggtaga gcgaaaagaa gaaaggaagt
    11581 acaatatgaa tagcgttttg attatagatg atgacaaaga actttgtgct cttatgaaaa
    11641 aatgtgttga gcaagaaaat ttatctgcta ttactgcaca ttgtggtgca gaaggtctac
    11701 atttgctcga taaaaataga cacacactct ctcttgtaat tttagacata atgatgccag
    11761 atatagacgg ttttcaagtt ctgaaaaaaa ttagagaaac aagtaatatt ccagtaatta
    11821 tgcttactgc taaaaatgac gaggatgata aagtttccgg actacgttta ggtgctgacg
    11881 attacctaac aaaaccgttc agcataaacg aacttatggc tcgtgtcaat tctcttattc
    11941 gtcggtatac tatgttaaac cctgctttta taacagacac agattgcata ataataaatg
    12001 gcatgattat tgacaagaca aaccgaattg tttctatcaa taatattcct gttcaactta
    12061 caagcaaaga atttgattta cttgcttttc tggcttctaa taagggacgg gtatttacca
    12121 aaaaacagat ttatacacaa gtatgggaag aagaatatgc ttttgacgat agtaacatca
    12181 tgtctttcat cagtaaattg agaaaaaaga ttgaaccaga ccccaaccat ccattttata
    12241 ttttgacagt tcgtggtgtc ggttatcgtt ttaataagga ggcttaacat gaatacaagt
    12301 gtctatctac ttttgatatt ggctcttagc ctgattatta tagcgtacct tgtttctgtc
    12361 ctgctacgtg tccgaataca acttacactt ataaaagatg ctttagagga tataaaaaat
    12421 ggaaacctta atcgccgtat tttaacagat gaaaacgaca ttacaaaaca aatttgctat
    12481 gatattaacg aaatagcaat caatagccaa tttcaactta ttcagcaaaa acaatcagaa
    12541 caagcctaca aacagctaat gacaagtctc tcccatgatg taaaaactcc acttgcttct
    12601 ttggtcggtt atttggaagc agtagaaagt aaaattgttg taggagagga aaaagacgag
    12661 tatatccatg ttgcgtctaa taaggctcat tacttaaaga attttgtaga aaatctattt
    12721 gaatgggtaa aattggattc taaggaacaa gtttttcatt ttgatatttt tgacttgaat
    12781 gaattatccc gtaatattat atctgattgg atacctgttt tagaaagcag tcattttgaa
    12841 tatgaatttg atataccaga aatagagtac ttcttacgta tagacgcaaa tgcatatacc
    12901 cgtatcatta ataacctatt acaaaatatt attacccata gtagtggcaa caaaatgaca
    12961 ttgagaattt ttgaaaacaa agagcaagct caaattataa taacagataa tgggaaaggt
    13021 atatcttcag acaatctccc acatattttt gaaaggttgt accaatgcga ccattcacga
    13081 gcttcaaagg gaaatggttt aggcttggcg attgcaaagg aacttattaa cgctcataag
    13141 ggaaatatta ccgccaacag cactcccggt atgggaacag aatttacaat attgcttcct
    13201 aaagccctgt aaaagtacga ggctttctta ctctaaaaca agataaaagc aagattatgg
    13261 caaggttttg gcaaggttca tgttttataa ttctaattgt agttgatttt gacgttgtat
    13321 caactatctt ttgtagaaac aaatttatta aaagtgagga aattatatat gaatgatttt
    13381 gtgattgaaa caaagcaact tacaaaaatt tatgggaatc aaacagtagt tgacaaagtc
    13441 aatctccatg taaaaaaagg ccgaatatat ggtctgttag gacgcaacgg agctgggaaa
    13501 accacaatta tgaaaatgat tttaggattg acttcaatca cgtatggcga ggtagatgta
    13561 tttggagaga atattaaggg atgtgaaaaa cgagtatatc cccgtatcgg tgcaattatc
    13621 gaaacgccag gattttatcc taatctgaca ggaacggaga acttggagat ttttgcaaaa
    13681 cttcgtggta cagccgcccc taattctgta aaaagagcat tggagattgt aggcttacct
    13741 tataaaaata aaaagctgtt tagtaaatat tcacttggca tgaaacagcg acttggtatt
    13801 gccaacgcta tcctgcatga cccggaactg ttagttttag atgagccaac caatggactt
    13861 gaccctattg gtattgcgga gatgagaaag tttattaggg acttgagcgt tgagcatgga
    13921 aagacaattc ttatctccag tcatattctt tcagaaatct cattgttggc tgatgatatt
    13981 ggaattatcg accacggtgt tcttttggaa gaaagtagta tgagtgaact agaaaagaaa
    14041 aacagtaaat atatcctgct acaagtttca gatattccaa agacatcact tattttagag
    14101 cgtcaattta acacgaagga ttattccgtt caagatgacc acactttacg cctttataac
    14161 actactctgg atatgggtga aatcaataaa gcacttgtaa tgcagaatgt aactgttatt
    14221 agttctcaac tttgtaatga tactttagag gactacttta aaaaaatcac aggaggagag
    14281 ggcattgctt aaacttattc aaacagaatt tcttaaactg cgtcgtagga aacttatttg
    14341 gctgatgttg ttgtctgctc tcattatgcc gtttttcgca ctactatact tcaattattt
    14401 tggtaaaaca ggtgttgagc caacaaagtt ttataaatgg tctgcatttg gttatacact
    14461 ttggattatt ttgcccgttg ttttgggtat attttgcacc atgcttatac acgaagaaaa
    14521 ccagtatgat atgctcaaac agctttggat tgtacctatt agtaaaatgg gatacttttt
    14581 tagtaaattt ttcgttgttt tgatttactc tatttgtttt atgctgatta cagccgttgc
    14641 ttctgtgtta tttagtgtac tttctggata tgtcgtattt agctgggcaa gcattttata
    14701 tttgttgaaa aaatgtttag aaattggtgt aatcactgct tttgtaatgt tgccgatttt
    14761 ggcaatcgct acatctcaaa aaggatacat tctcccaatt tgtatcacat tggtatatgc
    14821 cttttccggt tttgttataa tgacaataaa tatgtattta cacccattat ccagtatggg
    14881 agttattatc atgagaaata aagacattcc aggtcttata ttttcacagg aaattaatgt
    14941 cccattagca tttctttgta tttgtatttg gagtattgta tcagtattgt ttgcaagcat
    15001 tgcactagga agaagaaagt gaggtgtaga taatgtggaa attactttgg gctgaatgtc
    15061 aaaaaattcg tcgttcaaaa attgtttgga ttacattttt tgcatccatt atgatagcag
    15121 tcatagtttt tgcaggaggg caggatgtat ataatgaccc agatttacat tatggcttaa
    15181 agagcgttta tgacggttca cggtatatag acaatgcagg ttggtatatg gacgcggtac
    15241 aaccttggtc aacttttttt gttcttcctg ctgtcattgc cctattagga agctatatta
    15301 tttgtcgtga agaagaggag gacactatta aatccctgcg tcttattccc ataaatgaaa
    15361 caaaattaac catagcaaaa atgattatta cttttatatt cagtatactt ctttatttgt
    15421 tactctttac tataacattt ttgactgaat ccgttttaca tttttccgat ttatcgacaa
    15481 aactggtttt aagttgcatg aaagagtatt ttcttgatgg cataggtgta ttctttgcaa
    15541 tttctcccat tattgccttg gtatcgcgca tgaaaaaagg atattggctt gcattagtat
    15601 tcacagaagt ttattctatt gctggactat ttgcgggtat gtcaaatgtc ttacaaacct
    15661 tttttcctat tgcagcaata tttaatattt ctggatacta tataactact ggagaaaaaa
    15721 ttatggggag tatcataagt ttattacttt gtgcctgcct ttctgcattt atactgaaag
    15781 ggctaaaaca caatgaaaaa aattgaaata ggtatatcaa aatgaaaaag ataaaaaaac
    15841 tgattattct aattgtattc ttgtttcttt ttggcatgtt acactgttat actgaatgga
    15901 aattcaacaa tgacccacct aaaggggtaa tagaaagaat aaatgagtgg actgataaga
    15961 ttggactgga ttagtaactt atgttaatta tagtgaatgt aatattagca agttattgtt
    16021 tcgtatttta atactataac tttaaatatt tggttttaat cttccgaacg aatataaaaa
    16081 aactgtcaca gaggcaacat atttttgaat atttataaag tggtagtttt gattttcaaa
    16141 gctgccgcct tttcttctat ataagcaaac ttatcctcaa caaagtgtaa aacatggtac
    16201 attttaagaa aaaagtacca cagacacaag caataaaaga aacgccgaga acctacaaaa
    16261 acgtagtgct tatgcagatt tacgacgcct tctattttct caaccgtctt aatgaccttg
    16321 tacaaaagaa atttttctaa tattttttat aatcttatct tgtgatgttt tttatgtata
    16381 aatgacttaa taacaaatcc aataaaaatt atggatattg gtataattag aacgaggaat
    16441 ttataatcat tataatgtga cattattctt acccaactct cagaaaaaat atatcctata
    16501 ctgattaatg aagtattcca tatggttatt cccacgatag atgatattac aaaagttata
    16561 atattttgac ctgctatacc tgctataaaa gatatgtatg ttctacataa agggattaat
    16621 ctacatacac aaacagaaat agaagagtat ttattaaaat aatcttgaga tgcaaatatt
    16681 cctttctttg cctttggaca tatctctatt attttattta tgatagattt acctccccaa
    16741 tttcccactg tataacaaaa tatcgaacca agtatacctg caattatact taaaattaaa
    16801 attgtcagaa atccaaaatg atttcttgca gcaattgctc cacataaagg taaaacaact
    16861 tcgctaggta atggaaaaca agcatactca atcataatta atacaaatac agagattaat
    16921 ccatattgta aaataaaact attgattaca gacacttact tatctatctc ctttcttatt
    16981 taaaggtttt aagggtacat attctaagga ttctcaccct atatttaaag aatcaatctc
    17041 tttaaactat tatatgtatt tacacaaagc atttataaca tactttaaaa atagtacctt
    17101 aaaataagaa acttaataaa t
//
LOCUS       DACTGW010000068        16664 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.068, whole genome shotgun sequence.
ACCESSION   DACTGW010000068 DACTGW010000000
VERSION     DACTGW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.068"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(247..1638)
                     /locus_tag="I9573_001758"
     CDS             complement(247..1638)
                     /locus_tag="I9573_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAT4796382.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(1649..2869)
                     /locus_tag="I9573_001759"
     CDS             complement(1649..2869)
                     /locus_tag="I9573_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAT4796383.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2883..3671)
                     /locus_tag="I9573_001760"
     CDS             complement(2883..3671)
                     /locus_tag="I9573_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAT4796384.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3688..4962)
                     /gene="larA"
                     /locus_tag="I9573_001761"
     CDS             complement(3688..4962)
                     /gene="larA"
                     /locus_tag="I9573_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HAT4796385.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(5080..5793)
                     /locus_tag="I9573_001762"
     CDS             complement(5080..5793)
                     /locus_tag="I9573_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAT4796386.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5918..7177
                     /locus_tag="I9573_001763"
     CDS             5918..7177
                     /locus_tag="I9573_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HAT4796387.1"
                     /translation="MKKNKIIAFFAPAIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKHEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFIKKFSRKK"
     gene            7445..8011
                     /locus_tag="I9573_001764"
     CDS             7445..8011
                     /locus_tag="I9573_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAT4796388.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(8212..9417)
                     /locus_tag="I9573_001765"
     CDS             complement(8212..9417)
                     /locus_tag="I9573_001765"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HAT4796389.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9520..9626)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9718..10086)
                     /locus_tag="I9573_001766"
     CDS             complement(9718..10086)
                     /locus_tag="I9573_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HAT4796390.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(10138..11415)
                     /gene="obgE"
                     /locus_tag="I9573_001767"
     CDS             complement(10138..11415)
                     /gene="obgE"
                     /locus_tag="I9573_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAT4796391.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11526..11816)
                     /gene="rpmA"
                     /locus_tag="I9573_001768"
     CDS             complement(11526..11816)
                     /gene="rpmA"
                     /locus_tag="I9573_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAT4796392.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11830..12147)
                     /locus_tag="I9573_001769"
     CDS             complement(11830..12147)
                     /locus_tag="I9573_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAT4796393.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(12162..12473)
                     /gene="rplU"
                     /locus_tag="I9573_001770"
     CDS             complement(12162..12473)
                     /gene="rplU"
                     /locus_tag="I9573_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAT4796394.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12574..13995)
                     /locus_tag="I9573_001771"
     CDS             complement(12574..13995)
                     /locus_tag="I9573_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HAT4796395.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLIIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(14013..14714)
                     /locus_tag="I9573_001772"
     CDS             complement(14013..14714)
                     /locus_tag="I9573_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HAT4796396.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14716..16560)
                     /locus_tag="I9573_001773"
     CDS             complement(14716..16560)
                     /locus_tag="I9573_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HAT4796397.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFI
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
BASE COUNT         5150 a         3112 c         1770 g         6632 t
ORIGIN      
        1 tatttataaa aatcatttta ttaactcttt attatataag atacaatagt cttactttat
       61 ttatgtacac atgcttttag gatgacaaca tcaacttaaa tattatgtta ctttctaaat
      121 ttaagtaaat attgccatcc taaaaatttt acaatttagc ttcagattta aaagtaaagt
      181 ttaaaatatt atatttaaat ctaatttaat attttttgtt ttgtaaatat tattaaatta
      241 atatttctaa tatattttcc ctggatttaa aatattttta gggtcaaaag ctaactttat
      301 atttttaatt aacatcatat aagcatctga atttgattca tgtaagtact cttttttagc
      361 atatcctatg ccatgttcac cagaaacttg accactaagt tctctagatt ttttatacat
      421 atagtcaaaa gtttctttta atcttatttt ccatgtatta tcatccatac catcttttaa
      481 aatatatatg tgtagatttc catctcctgc atgaccaaaa ctttttattc ttatttttaa
      541 tttgtcttga agctcgtgag tgtatcttat aaactctgct atcttatctc ttggaacaac
      601 gacatcacat tcatccattt gtgtcgtaga tgcctttata gcttctaaaa atgcacctct
      661 ggctgaccat atagaatcgt ttctttcttg tgtatctgat ataaatacat ctaatgcacc
      721 attttctaaa cataaattag ctactttttc atattccttt tctatatctt cagtgctatt
      781 tccatcaaat gtaagtaaaa gataagcatc tgatgtatta tctggaaact ttttacctaa
      841 aaattcttct gctgccaata ttacatctct ttccataaat tctatagccg ttggtattga
      901 ttttgatttt attattttag gaacagtttc tattgccatt gataaatctg gaaatggtat
      961 aagcaaactt atacttttct ttggtaaagg aagcaatttt aatatagctt tagtaactat
     1021 tcctaaagta ccttctgaac ctactaataa atcctttata ctatatccag aactattttt
     1081 aaccaccttt ccacctacat ttataatttc tccatttggt agtacaactt caagtcctct
     1141 tacataatcc cttgtaacgc catattttac tgctctcata ccacctgcat tagtgtttat
     1201 attacctgca attgtagcac ttttttcacc tgggtcaggt gggtaaaata aatctctatc
     1261 ttgaacatat tgacctattg tcatcaataa aacacctggt tctactgtca atgttaaatt
     1321 ttcataatca acttcaagta ttttattcat tttacataga ttaatcatta ttccaccatt
     1381 tatggcaaca gcagcaccta caagaccagt accttgacct cttggtgtga ctggtatatt
     1441 atttttatat gcgtatttca ttattttaga tacttgttca gtttcaagta catttactaa
     1501 aacttcaggg tacttctcaa tccctccaag ttcatcatgt gaaaaatctt catttatatc
     1561 acttccaact aatacatttt cttctccaca agtatctatt aaaaaatcta tatctttttt
     1621 atcgattaat ttatacattt ctatccccct atttagctgc tgatgccata tatttttgat
     1681 tattatcttt gttattttct attttttcaa tcaatttagg aataatatca taaatatcac
     1741 ctattaaagc taaatgtgct acatcaagta taggcgcttt atcatcttta tttattgcta
     1801 ctatatagtc agcaccctgc atccctgcaa caaattgaac tgctccagat acaccacaag
     1861 ttattatcaa tttaggtttt acagttctac cactaagacc tatttgtttt ttagcatcta
     1921 tccaacctgc ttctattaca ggtcttgttc ctgccacttg agcgtttagc ttatcagcta
     1981 atttatatat catctccata tcttcttgtt tttttattgc tctgcttgcc acaactatca
     2041 cttcagcttc ttccagacca acttctttat ttttttcttt tacactcagt accttaattt
     2101 tagattttag ttcttttttt tctactttac ataaagttat ctttcctgta gtattctcta
     2161 ttttttctgg tgctgaaaat attttatatc ttacagtggc aaattgtggt ctattattag
     2221 gtgtgtttat atgagccatt atatttccac caaatgctgg tcttatttgg tctaaatcag
     2281 tatttgattg aatatcaagt attgtacaat ctgctgtaag acctgttcta aatctagcgg
     2341 caagtcttgg agctaatgac ctacctaatg tagttccacc aactaatact atcgttggtt
     2401 ttattttatc tataaagttt tctattgctt ttgaatatgg ttctatccta aaatctttaa
     2461 attcatcttc atcatataca aatacttcat ccaccccata agaaatcaaa ttactgcact
     2521 tatcttttat gtcttttcct acaaatacac aatacacagg ttgttttatt tttgatgcca
     2581 tctcacgagc tttacctata agctcgtacg ttacaggatg tatatttcca ttatgatgtt
     2641 caacatatac tgcaattccc ctccattcat ctttattaat ttgtacttta ctactctcta
     2701 taaattcaaa tgcgcctttt ggtccacttt tgacacacaa cttgcacatc ttacagccag
     2761 cacttatttc tactatacct gacttttctt ctatagcccc aaaaggacat atttctaaaa
     2821 ttctctttaa gtcagttatt ttatcttgat ttacaactat ctttgacatt tattactccc
     2881 ccttacacca actttaattc ctctaattta tttacaagtg caaaacttag ttcatctgca
     2941 tttcctctta caatattttt atctgtattt ggctttggag gaaatattct tacaacttga
     3001 gtaggagaac cgttaagtcc atagttattt tcatctttat cttcaaagtc atctaagcta
     3061 tatacaggta tttctctatc tttagtatta agttttaatt taaatgaagg tagtcttggt
     3121 tgaaaaatgt ctttttctac agttattaaa catggatatt caatctcaca tacttgtaaa
     3181 tcatttggca tatctatttc aacaactata ctttcatctt taacttcaat taattttgta
     3241 acgttagtaa catgaggtat atctaaaaat tcagctactt caggtcccac ctgtgctgta
     3301 tcaccatcag tagtttgttt tccacaaatt attaggtcta cctttcccat cttcttaata
     3361 ccttgtgata tggtatagga agtagccaat acatctgctc ctccaaatct cctatcactt
     3421 attaatgctc cttcatcagc tcccatcata aaactttctt caattacctt ctttgcctga
     3481 ggaggaccca tgctaagtac ttttagattc gctttcttat cttcctttat tctaagagct
     3541 gtttctaaag catataggtc atagggattc atttttgaat ctactccatc tctttttagt
     3601 actcctgtag tttcatcaac ttctactgaa gtcgttccag gaacttgttt aatacatgtc
     3661 aaaatttcca taaagtacac tcctttttta tttaactata actgatactc catcaggtat
     3721 aactactact ttgctctcct taccaactat actattcgcc atttctaaag cttctttaaa
     3781 gttaaatgca tgtttcatat gcattttttt tatcatttca ggattacact tatctgttac
     3841 cattatcaca gtatatttag ataatattct cgctagtatc tgaaattccc attggtctgg
     3901 aatcgtatct actctatcga cacttctaat tttatctaaa agttgttttg gagaatctgc
     3961 atttgctaca ttttcataaa aagattgacc tccatgacca tcattacatg ctgaaatcat
     4021 tattattact ccaccgtctt tacaagtagc ttcagctgct gtcattcctt taacagattg
     4081 ataaatattt tggtctagtg gatatccacc atttgtagtt acaacaatat cagattttat
     4141 agaatttact tttgataact ctgttacaaa tctacatcct ttagtatgtg ctttttcgac
     4201 atcaccagca aaggaagcaa ttattttttt atctttatct attacaacat tcaatatgaa
     4261 tgctaacttt gcaactttag ctgcatatac catatcctca tgaataggat tattattcaa
     4321 tttcccagtt cttgaattat cactatctat aaagtcacta cagtgattgt acattatagt
     4381 ctttgcagat gcgattcctg gaagtacgct ttttctgcca cctgaaaaac ctgcaaagaa
     4441 atgaggttct ataaaacctt ctgctatcaa aagttctgcc tcatatgcta gtttgtttat
     4501 atataaatct ccacctgaag gcaatatccc tactttacac atagatttat catcagtaga
     4561 tatatgcata actatatctt cattatctac tatttcttct cccattttat aaattagctc
     4621 ttcccttgta cttggcctat gaaatccagt tgccactata atcttcacat caatttctgg
     4681 attaacattc ctaatccttc ttaatataat tggcatagtt attctacttg gtacaggtct
     4741 tgtatggtca cttgtaatta tgactacatt ctttttcccc ttaaccagtt cttccagaga
     4801 attagagcct ataggattat tcatagattc ttcaactatc tcttcttgag tcttatgttc
     4861 tatataatta tccgattttg actccaaaac accgagaaaa cgttcatcag gtatttctaa
     4921 aatcattcct tgttttgaat acggtacttt taactttacc atttttaatc tcccattata
     4981 ataaactata aaattggtat gtggtcatac cagtttcaca tttatatgat agtaccatta
     5041 tctaccattt gcaagaataa tttattttat aacaaacttt taattttcat tttttctata
     5101 atttttactt ataaaatcca tatgttcctt tgctttctca catgcaagtt ctacatcatt
     5161 ttctacaact gcattgtata tagcttcatg ctgtttaagc aatatatttt cattaaaagg
     5221 ttctgcgatt attttttctc ttgcatcttc tataaatcca tcaaataatt ttgatgctgt
     5281 cataaaaaga ctttgaatta aataattttt acttaatgtt gctagtttac tgtgaaattt
     5341 ttggtctaat aacaccagta ctcgattatt atctttttta ttagtttcta ctctcatttc
     5401 atctattata tgtttcaatt ctataacttc agaagaagtt attctctttg aagcaagttt
     5461 tgcaatctca atttctagga ttattctaag ctctaatata tcttcaagtg tgccattatt
     5521 aagtttaaaa atcatagata aaggttctat taaagttttt tctatatttg tacatataaa
     5581 atttccttca ccctgtctgc tttctatcac tcccatagtc tctaataccc taatagcttc
     5641 ccttatggaa gttctactca cattcatttg ttcagacaat tctctttctg atagtaattt
     5701 atcccccttt tttaactctc catttagtat tttatattgt atttgctcaa ttacttgttc
     5761 ataaactttt ttactagcta tactattaaa cattttttcc tccaatatga taatcgttgt
     5821 tgtaatatat atctattata tatcttaaac cattacattc atatattttt attactttta
     5881 atataatatc ttataactca aatttacagg tgatattatg aaaaaaaata aaattattgc
     5941 tttttttgct ccagctatta tagttggagt tctagtttta gggattatcc tatacccaaa
     6001 tgaagctata aaatctgcaa gagaaggctt ctctatatgg tctaatgttt tagttccatc
     6061 actactacca tttataattg gagcaaactt aattgttgat ttaaaaatag tggatattat
     6121 agggtttatc atcaatccca taactagatt tgtatttaat gtttcaggaa agagcgcttt
     6181 agcttttgct atatctacag tatctggtta tccagtagga gtaagtttag cttctgaatt
     6241 taggagtaat gggcaaattt ctaagcatga agcacaaaga cttgtgtctt tttgttctac
     6301 atcaggacct ttattcataa taggttcggt tggtacaggt atgttccaaa attcaaactt
     6361 aggttattta atgattctct gccattatct aggaactata ttggttggtc ttttctttag
     6421 aaattatggt aatgaaaatc ttccaaagac aaaattggat ttaaaaacaa atataaaaaa
     6481 tgtaatcaac attagaaatt caagtgggaa tggttttttt gttttatttg gaaatgctgt
     6541 atttaatgga gtaaatacat tacttacagt tggtggattt gtaattgttt tttcagtagt
     6601 atttaaaata ctatctcttt ttaatgtaat ctcattaata gcatcagtta tatatttgcc
     6661 tctttcattt ttaggtgtta gtaaagaact ctgtcaagca tttgtaagtg gtctatttga
     6721 aattactata ggatgcaata aagtatctag tataacaagt tctccagaaa ttttaagagc
     6781 atctttagct agctttttga taggatttag tggattatcc atcctagctc aatgttgtac
     6841 atttctatca aaaacggaca tagatttaaa attatacata ctaagtaaat ttgcgcatgg
     6901 agttttagct gctatcttta tatttatact ttatccaatt gcaaactctg ctgtgttggt
     6961 ttctagcttt gctgatacat acaacgttat atataataat ttaatctggt tttattatct
     7021 tagctattat gatacaattc ttcaaattgt agtaataatt tatctaatct cagcagtatt
     7081 tattgcaaaa aaacatatga aaactttatc tacaaatggg aaactaatgc gatataaaaa
     7141 tacttttttt attaagaaat tttcacgaaa aaaataaaac taataaaact taactaaata
     7201 cttccatttt tttattatat ttgtcataat tttaataacc tttttaatta gtttaacagc
     7261 atagttattt catcacaatt taataatttt attagaaata tttttcaaaa aattctcctt
     7321 aatcacatat atcacacttg tataagttta ctgggaaatt tttccattat ataatgtttc
     7381 atttatatta tataataaat accgacctaa acttaccaaa tcaaactaag aggagtggtt
     7441 gaaaatggca acaagaccta tagaaataga cgaatataaa aagataatgg aactattaca
     7501 tactggcttt acttatagtg aaaatggcgt tgaaaaaaga tttagaaaaa atcctaaagt
     7561 tgcattggca ctaatgttag aagctaattt aggtctaaga atatcagata ttttaagatt
     7621 aaagataggt aattttaaag gtaatatgtt ggaaataaat gaaaaaaaga cgggcaaatt
     7681 acagtataga ccagttaata aaaatataat tgagtccata aatgaacatg caagaaagta
     7741 taatcttaaa tcaaatgact acttagtaaa tataaagaca aaggctattc aaaaacagtt
     7801 gaggataatt tgtaaacact taaatctgta taatataagt acacatagct ttagaaagct
     7861 ctatgctact actcaatttg aaaagagtaa caataattta gagttggtta aagaactttt
     7921 aaatcacagt tctgtagcca ctacacagcg atatataaga gtaactcagc aagctattaa
     7981 tgaagctagt gaaaatttct ttattggtta attataacgt atacattttt ctattatctt
     8041 attaattcac cagatattaa aaaagagtgt ctcaaaatga actttttagt tcatgagaca
     8101 ctcttttttt ataaaatcaa atatattttt attatttcaa tactggaaaa aaggcttgtc
     8161 tctctatttt gagacaccct attaaatttt cataattctt gcataaacat attaatctat
     8221 cttcaataat tttttagctg attcactgta attatttctt tttacaattt ccaaaactct
     8281 atcaatctca tgttctgact ttgcacttac aaaaatcgcc aattcttcat catctataat
     8341 atcataacta tctacatatt tatctaaaca ttctaaaatt ccatctttat tattataccc
     8401 accaaatcta actactgcac tacactcatc ttctataagt ccacttaaat ccaatttccc
     8461 ttttgaaaaa gactttatat ttgaacttct attatctatt atatctataa tatctgcata
     8521 aacagcactt cctgttgcac ctcttccagc tcccttacct acaaaagaaa gttcacctac
     8581 tgaatcacct actaaaataa ctacgttgaa ctcattatct atctttgaaa gcatattatt
     8641 attgtccact aatgctggtc taacaaatcc acttacttct cctgattcat acttacttct
     8701 agcaaccaat tttatcttac aatttaattt ttttgcatat tctatatctt tttcatctat
     8761 tgaagttatt ccttctaata ataaatcttt ccaatatact atttttccat aagataaagt
     8821 agacaatata gataactttc tagcactatc atatcctaaa acatctgcat ctggattaac
     8881 ttctgcaaat cccaactctt gtgcctgttt taaagcaaca tcatatggta aattttcatc
     8941 atacatcttt gataatataa aattacaagt cccatttaaa atagcgttta tgctctttat
     9001 attgttgcct tccagtgatt ctatcaatgg ttttaaaact ggaataccac ctgccacaga
     9061 tgcttcaaac ttgatgctaa cattatttaa gttagctaat tctataaggt cactaccttt
     9121 ttcagccagt aaatctttat tagctgtcac tacatgtatt tttttattta acgctcttgt
     9181 tacataatca aaagctggat ttaatcctcc aattacttca actagtatat caatatcctt
     9241 gtcaaatata tcttctatat cagtagttat cttatcaaaa tacttcttat acttgtgttt
     9301 ttctttattt ttaactaaaa ttccaacaac ttctatatct ctttttgaag tattattttc
     9361 tattaaatca attaatccac ttcctactgt tccataccct aatattgcta tcttcactgt
     9421 aacatacccc catcattttg attatattaa attatttata tatataaata taccaataaa
     9481 aaaagcttga gtataaactc aagccatatg acgggtttat actcatcttt cagcataaat
     9541 gctgcaggat ttagcaccaa tgtataatta aatatactgg ttgccgggct tcacagggcc
     9601 agtccctctg ccactcttga taagattgct aattaaaatt tatgtttaat agtataatac
     9661 aatttcttcc aattgtcact atttttttaa cttatttccc taaaaggata taaactatta
     9721 tctaacagct cttttagttg gtttaccctt ttttgtaaca ttattatttt tagtctttgc
     9781 tttcgcctgt tcatgtcctg gaaaaagtat tttaggctct tcaatattcc tcttgtaaat
     9841 agtaaattta aaaccaatag cctgcacaaa ttcagctctt aaagcttcac atactgcatt
     9901 agcagtttct ttagtatcaa gacctgcatt ttctaatata gtaactttta ctaattctct
     9961 agttcttaac ataccatcta attgctctaa gaaactctct gtaacacctt ctttaccaat
    10021 ttgtgtagta ggctttaaag tatttgctaa agatcttaaa tacgcccttt gttttccttt
    10081 taacatattc tcacctctat aattctatac ttaaattatc tataatctat atttaaatta
    10141 attgtaaaac tcaaattcta attcataaat tttaaccaca tctccatctt ctattcccat
    10201 ttctctaagc ctatcaaaaa caccttgact ctccatagct ttttggaaat attgaaggga
    10261 ttccatatca tcaaaattta cagaatacat tattcttcta agtgctttac ctgtaactac
    10321 atacacacca tcttcaatat ctatacttaa ctcttcttca gtaccaatat caagttctgg
    10381 tctatacatt tcttcttctg aaactagctc cacatcttct acttctttta gcattttaga
    10441 aacataggct attacttcat ctatgccttg acgagttgct gctgacattt taaatactgt
    10501 atatcctctg ccttctaatt cactcttaaa cttctcaaac ttactctcat cttctaatat
    10561 atcaaattta tttgcaacta caacttgtgg cctctttgaa agcttttcat tgtacaattt
    10621 aagctcatca tttatcttgt caaaatcttc aatagggtct cttccctcta atccagatat
    10681 atctacaata tgtatcaata cctttgttct ctcaacatgt cttaagaact catgacctaa
    10741 tcctatgcct tcagaagcac cctctataat tccaggtata tctgcaagta caaaactatc
    10801 tccaaactta gtttgaacaa cacctagatt tggtgtaagt gttgtaaaat gatagtttgc
    10861 aattttgggt ttagctttag ttacaactga tagaaatgtt gatttaccta catttggaaa
    10921 tcctaaaaga ccaacatctg ctatcatttt aagttcaaga gttatccatc tttcttctcc
    10981 atctgtacca gattttgcaa aagctggagc ttgtcttact gcatttgcaa agtgttggtt
    11041 acctttacca cctataccac cttttgcaac tatagctgta tctccttctt tttttaaatc
    11101 tgctaataca agacctgtag cctcatctct tattactgta cctgctggta cttttaatac
    11161 taaattttct ccatttttac cagctctttt tcctttgctt ccgtcgccac cattttgagc
    11221 ttgatatttt ttctggtact taaagtccat tagagttcta agccccaaat caacttcaaa
    11281 tatgatgctt gctccacgac caccatctcc accgtctgga ccaccagctg gaacgtattt
    11341 ttctcttcta aatgctaccg aaccatttcc accatttcca gccttaacaa atattcttgc
    11401 cttatctata aacaaattct tcacctactt aattaaaaac ttttaattag cattaccaaa
    11461 aataccatta taaattcatc agtattataa gaaaaaggag tgcctcaaca ctccttttat
    11521 gtatattact cagctattgc tactggatat atactaactt tttttctctt cttgtctttt
    11581 ctttcgaatt taacagtacc atcaactaat gcaaataaag tatcatcact accttttcca
    11641 acattagttc ctggatgtat tttagttcct ctttgtctaa ctattatact tccagcagtt
    11701 actaattgac catcgaatct cttaacacct aatcttttag atatagaatc tctaccattt
    11761 ttagaagaac ctaccccttt tttagatgct aataattgta aattcatgtt taacatatgg
    11821 agtcaccccc tactttttta aaatttttat attttttggg tattcccgtt taatttcttt
    11881 aaccgctaat tgaaagtgat ttagtaataa ttgtgcgtct tttacatgcg tatctttgac
    11941 tatacactct atatgacctt cttctcctac tactttttca aattcaatct tttgtaattt
    12001 gtctaaagaa tttataactg caatagcatt agatgtaact gcagcacata ctatatcata
    12061 gcctatttca gcaaaatcag catgtccctt taaacaaaaa ccttgcaaca agaaatcatc
    12121 attataataa tatttaactt ttatcataac ttaaccaata actaagcgtt tattttttct
    12181 acaactatct tagtgtatga ttgacgatgt ccttgttttc ttctgtaatc tttttttgct
    12241 ttatacttga aaactataac tttcttagct ttaccttgtt caacaacttt agcttgaaca
    12301 gatgctcctt ctactactgg agaacctaat tttaattctc catctttaga gcaagctaat
    12361 acttcgttta aagtaacaac atcacctgcg ttagcttcta atttttcaac gaataataca
    12421 tcaccttcag aaactttata ttgctttcca ccagtcttta ctatagcgta cattaaatac
    12481 acctcctcgg cctcaaactc gccagtacaa ggtacttcat ttgaagattt aaacctttac
    12541 tgtgcggcat tacaattatt aattataaaa tatttacatt ttattgtcaa ctattttatc
    12601 gaaaataaca ttggttttat tgtgctttaa tgtactattt gcgtttaaag aaatctttat
    12661 attatatttt tcaccaattt tatcaattat atccatataa tattctttta tttgttcaaa
    12721 aataaaatca ttaaattcta ccattatgtt tttgtaaaca gtatgttcac ttattctcat
    12781 aatttctttt tcaatactat ctagtatata atgtacagat tttattgttt gttcaccatc
    12841 acaacatgga caagacatta aataatactt atcaatggat tctttttctc tacgcctagc
    12901 aacttctact aaaccaagtt ttgtcatacc taatacttca gcttttcttt tatctttatt
    12961 aaattcttct tctaggatgt gtaaaatctc cttttgatgc ttctttttat gcatatcaat
    13021 aaaatcgatt attattatcc cagctatgtc tctaattata agctgtctta caatttcttt
    13081 tgcagcttca agattagttt tataaacggt ttcatcaagt ttaccagttc caataaactt
    13141 acccgtattt acatctataa ccgtaagagc ttctgttttt tcaattatta aataacctcc
    13201 actctttaac catacttttt tatttaataa tttttctatt ttagattcta ttctgtataa
    13261 atcaaataca tctctatttt cctcaagaca cagtttatca acataagtct tatctatacc
    13321 tcttaaaata gacttaagtt cagagtactt atcataacta ttggtaatta tagattcaat
    13381 atcatcattc acattatctt ttacatattt agttgcaaaa tctaaagact tatataataa
    13441 tttcggacct ataccaagtt tatattcttt taaaatattt tcatacttta atttcaactc
    13501 ttctatatct ttctttattt catcatgagt gcaaccttga gcctcagtcc ttattattaa
    13561 tcctagattt tccttattta ctgccttagt aatttttttt aatctaaatc gttctttttc
    13621 atcagtaatt ttatttgata ttgtagttct atcattggaa ggtatataga caatatacct
    13681 tccagaaata ctaatctcag tgtttaattt tgcgccttta gttcctattt cttctttatt
    13741 tatctgaacc aaaatatcct gaccagattt tatagtactt ccctttttta attgtaaata
    13801 agcaagtttc ttaaatccaa tatctacaaa acaagcttct attcctggta taactttttt
    13861 tacgattcca cgatatatat tggatacagt cttcttattt aaattgtctt caacaaaaag
    13921 ttctgttaat ctttcatctt caagtactgc tgttttctgg gaccctatta gggactcaat
    13981 aactatcttt ttcacaactt cacctctcga gcttacatag gagtcactaa ttccccatct
    14041 tctaatacat ataaatctct tctcaatata tcaacatcta atgggtcaat atcaagttca
    14101 aacatctcta atatctttgg tataagtata ttagtattta aattagtttt tgagcctgta
    14161 gctatagtag cagtaagagt aatatgttca tcttctaaat ttaaaacatc aaaggttctt
    14221 atcataggtc ttatatctac ttcaaccatc ttaccttttt tattcttttt tgttatgatt
    14281 atttctttat tattcataaa gtcaattact tttcttttta caaactcttt cgtcataggt
    14341 cttttcaaat ctatattaaa caaatattct ccatagtcta tcacagaaga aagagaaggt
    14401 gtttgtctat caatttcttt tgctttgata aaatctatcc catctggaag ttgaattgat
    14461 actttattta aaaattcctc tactgataaa tcatcttctt ctatttcaat atctacatac
    14521 tcaccttgac tttctgtccc taaagccaaa gcattgccat aactcatttt agggtgagga
    14581 ttaaatcctt gagaatgtga taagtttatt tcagctcttc taaacgctct ttgtaacaat
    14641 ctctgaaggt caagatgtga tatatatatc atgtcgcctt cctttttaaa ttttactctt
    14701 attatcttac tcatattagc aaagccccct tcctatatca tgtatattta ttccacaacc
    14761 attgcagtta tgtctacaat cagaagtgat tttttctttt ttagcatttt cgtcttctct
    14821 aattaagaat tttttactaa tacctacatc tatatggtcc caaggaaata cctcttcata
    14881 gtttctattt ctatgtgcat aaaaatctat agaaagatta ttcttttcca tagcttcttt
    14941 ccatatatct aaattaaaat gttctgccca tccatcaaat ttagcaccta gtctaaatgc
    15001 atcatatata acttttccta ttcttctatc gcctctagct actactgctt ccatcaaact
    15061 agtctttgaa tcatgataat tgtattttat attgttattt cttaatttat ttactaaatg
    15121 tctctgctta ttaataactt cttctgtagt atcttgacca tgccattgga aaggtgtaaa
    15181 tggctttggt acaaatgtag aagtactcac tgtaacacta aaacttctct ttagctttcc
    15241 tccatttact tttctataaa tatcaataac tttataagct aatttagcta taccatctaa
    15301 atcttcataa gtctcagttg gtaaaccaat cataaaatac aacttaacac tattccaccc
    15361 catctcaaaa gctctttctg ttgcattttc caaatcttct tctgtaacac ctttatttat
    15421 aacatctcta agtctttgag ttcctgcttc tggagcaaaa gtaagtccag attttcttac
    15481 ttgttgtatt ttatcagcaa tttccattga aaaattatct aatctaaggg atggaagcga
    15541 aatcccaata ttattagatg catattcatc taccaaataa tcagtcaact ctgataactt
    15601 actataatca ctcgtactaa gtgatgataa tgatatttca tcatatccag tattcttaac
    15661 taatttatct aatatttctt taagtctttt aacactcttt tctcttattg gcctgtatat
    15721 catacctgct tgacagaatc tacatcctct agtacatcct ctaaataatt ctagtactat
    15781 tctatcatga acagtatcta tataaggtac tattagtttt tctggataat ctacagtctc
    15841 aacatctttt attatccttt tagtaggatt tttagtaatc ccttctctat taggagttac
    15901 actttgaact gtaccatctt cattgtactt gacatcataa aaacttggta tgtatactcc
    15961 ttctatgcta gatattttat ataaaaaatc ttctctagtc gttttatttt tcttccattc
    16021 tttatactca tttactactt ctaaattaac ctcttcacct tcacctaata ttactacatc
    16081 tataaaatct gctaatggtt cggaattata agcacaagga ccaccaacca ttataaatgg
    16141 gtcttccaaa gttctttctt cttttaatat tggtatattg gctaaatcta atatatttaa
    16201 gatattagtg tatgaaagtt catattgaag tgtaaaagtg acaaaatcaa agtttgttat
    16261 aggctctctt gactctaatg caaataatgg tatacttttt tctctcatta tattttccat
    16321 atcaactgca ggagaataca ctctctcaca aaatacatct tcatccttat ttattacatc
    16381 atataatata tgactcccta agtgactcat accaacttca tataaatctg gaaagcaatg
    16441 tgcatatctt atcaattcac tattactagt atctttatga attgaattta tttcatttcc
    16501 aagatatcta gcaggctttt ctactttttt taagattttt ttcaagtcta ctttattcat
    16561 ttattaaaat tcctccaaac aagctcttta tttttattct tatttataag tagtattata
    16621 cacgataaaa tattttctat caaaaatata actaaaatat tgaa
//
LOCUS       DACTGW010000069        16602 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.069, whole genome shotgun sequence.
ACCESSION   DACTGW010000069 DACTGW010000000
VERSION     DACTGW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.069"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            272..985
                     /gene="ispD"
                     /locus_tag="I9573_001774"
     CDS             272..985
                     /gene="ispD"
                     /locus_tag="I9573_001774"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAT4796398.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            982..1467
                     /locus_tag="I9573_001775"
     CDS             982..1467
                     /locus_tag="I9573_001775"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAT4796399.1"
                     /translation="MRIGLGYDVHKLTENRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     gene            1834..3549
                     /locus_tag="I9573_001776"
     CDS             1834..3549
                     /locus_tag="I9573_001776"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAT4796400.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDQCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            3671..5116
                     /locus_tag="I9573_001777"
     CDS             3671..5116
                     /locus_tag="I9573_001777"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAT4796401.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKTKGVAVEVDDRDNYT
                     TGWKFNEWEMKGAPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            5556..7037
                     /locus_tag="I9573_001778"
     CDS             5556..7037
                     /locus_tag="I9573_001778"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAT4796402.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLVEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEITEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            7558..8955
                     /locus_tag="I9573_001779"
     CDS             7558..8955
                     /locus_tag="I9573_001779"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAT4796403.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKIFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            8940..9353
                     /locus_tag="I9573_001780"
     CDS             8940..9353
                     /locus_tag="I9573_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HAT4796404.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            9460..9564
                     /locus_tag="I9573_001781"
                     /pseudo
     CDS             9460..9564
                     /locus_tag="I9573_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590469.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase (FAD)"
     gene            9861..10691
                     /gene="thyA"
                     /locus_tag="I9573_001782"
     CDS             9861..10691
                     /gene="thyA"
                     /locus_tag="I9573_001782"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAT4796405.1"
                     /translation="MSLADKIFIDMCNNILENGVSSDGEVVRAKWDDGQPAHTIKKFC
                     VVNRYDLSKEFPILTLRPTNLKAAIDELLWIWQKKSNNINDLNSRIWDSWADETGSIG
                     KAYGYQIGQKHKYKEGEFDQIDRVIYDLKYNPYSRRIITNIYNHNDLNEMNLYPCAYS
                     MTFNVTGNRLNAILNQRSNDILVANNWNVTQYAILVHMLAQISDLEVGELVHVIADAH
                     IYDRHIPLVKELINRESYDAPKLIINPNIKNFYDFRVEDFTLENYKSGPQIKKIPVAI
                     "
     gene            10704..11198
                     /locus_tag="I9573_001783"
     CDS             10704..11198
                     /locus_tag="I9573_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAT4796406.1"
                     /translation="MKVIVAVDLNWGIGYGGKLLQSIPEDMKFFKEKTVGNVVVMGRE
                     TFKSLPGKKALKDRVNIVLTRSKSFNDDRLIVCNSIDETLKELQKYENDKIFIIGGET
                     VYKQFLPYCNELYVTKIQKTYEADRFFPNIDIMSNWDLIKESEVKEYNNIRYVFSTYI
                     NNSL"
     gene            11225..12601
                     /locus_tag="I9573_001784"
     CDS             11225..12601
                     /locus_tag="I9573_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HAT4796407.1"
                     /translation="MKKDIFVNRVSNALENDRGAVFVGSGISFSSTEVDWFKLLEPLV
                     NELDITLDNKSDDLPMIAQYIVNNYSGNRGPLVNEISKTFNRKFKLNKYHKYLATTKL
                     STIWTTNYDMLLEDAFYDFDTSVKVSDDSITRNRLEYDVEIIKMHGCISRSDWNEIVI
                     TQEDYDDFSINKPAISQRLSEDLINKSFLFIGYSYRDPNIRNIMINARRLSKKHTQQH
                     YIILKKLKDSDSNITQDDVVRQELWCNDLKRLGISTLFIDEYSELEELLREISQKSRG
                     KTVYVTGSHKESNMNARKLGTKLAKHKDITLLSGQSSGIGSEVVAAFTEECIKSKEDI
                     INRMKIFPNPYSADEKYSDDITLIPDLKKCRTKMLNSTQVVVVFSGGIGTEAEIDVAI
                     KQNCNIIPVITCKEDRENPAIKKILNYSDNMKNIEIKDATYYNKIVDEDGVVTMEEIY
                     ICISKIMN"
     gene            12577..13677
                     /locus_tag="I9573_001785"
     CDS             12577..13677
                     /locus_tag="I9573_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiC"
                     /protein_id="HAT4796408.1"
                     /translation="MHIKNNELKHDIIKLKKRNGFIEVGKDKKTLVNCLIGANELEDY
                     KYEIKKIDCILNISEKPDIVSDLSTKRRKISESIWYKIVKDTPFVSATLPIYLVSKNN
                     NKIDSNELLDIIIEQMENGVGMITIHPTANKQIFEDSKNRMVPVTSRGGGMVIQDLIS
                     KNFSEDNVYLKILPEIIKYAQKHNVCISLGATFRSGNIFDSNDRAQITEIKSQIELAK
                     RIYKSNVGVIIESPGHSRPSDIIKISSILKKEGFPIMPLGPIPTDIAIGMDHIAAAIG
                     ATIMGIEGCANIISTVTREEHTGGRPSIESTVESIRTAKIAAHIIDIHQMNDINLDMS
                     VSKSRAKSNTCIFGKDTRYCDRCRELCPLCIR"
     gene            13720..14145
                     /locus_tag="I9573_001786"
     CDS             13720..14145
                     /locus_tag="I9573_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HAT4796409.1"
                     /translation="MHRIASFSQQSQRYVKLEQFEYIIPPEIEKIEKAKELFINSMKK
                     YQEDYDKLVEILFENHYNKLIKGGKNEKTSKRQVEKKAIEDARYVFPNACETKMVFTI
                     NARSLFNFFEHRCYENGSCLEGTMTCGDIVQVRKKFKNL"
     gene            14158..14895
                     /gene="rlmB"
                     /locus_tag="I9573_001787"
     CDS             14158..14895
                     /gene="rlmB"
                     /locus_tag="I9573_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAT4796410.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            14898..15425
                     /locus_tag="I9573_001788"
     CDS             14898..15425
                     /locus_tag="I9573_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAT4796411.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            15490..16137
                     /gene="sigH"
                     /locus_tag="I9573_001789"
     CDS             15490..16137
                     /gene="sigH"
                     /locus_tag="I9573_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HAT4796412.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            16211..>16602
                     /gene="tuf"
                     /locus_tag="I9573_001790"
     CDS             16211..>16602
                     /gene="tuf"
                     /locus_tag="I9573_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838150.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAT4796413.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVP"
BASE COUNT         6916 a         1699 c         3167 g         4820 t
ORIGIN      
        1 taaaacctct aatttgataa aattgtctta tatcaaacta gaggttttta atattcttgc
       61 atattaacaa ccattacaaa attgtaaaat taaaaacaac actattgaaa aatcaatata
      121 tattgtaata aatatatgtg tttttcatta gcaaatagac attaactatt tatacaactg
      181 aaaatttaat atataattaa tcagatggtt aaaataaaaa tattaaaagt tttaataaat
      241 gatggttaaa aataaaatac gaggtgatac catgtacagt gttataattg ttgcagcggg
      301 aagcggaagg agaatgaact tagatataaa caaacagttc ataaaattaa gagaaaaaga
      361 aattattgct catacaattc aagtttttta tgaaaatata aatatagatg aaatagtggt
      421 atgtataaaa aaagaagaag aggatttttt taaagaaaat ataataaata agtataactt
      481 taaaaatata aaaatagcat atggcggaaa agaaagacag gactcaatat ataatggttt
      541 aaaaaaatta gatgaaaatt gtgatatagt acttatccat gatggagcaa gaccatttgt
      601 cgaccataga ataatcaacg aatctataaa ggtagctaaa gaaaaaaagg ctgtggttgt
      661 aggtgttcca gtaagtgata ctataaagat agtgtcagat ggaacagtac aagaaactcc
      721 agaaagaaat ttgttgtggg cagcacaaac ccctcaaacc tttgaatata atctcataat
      781 agatgcttat gagcaagcat ataagaacaa ttattatggt actgatgatt caatgttggt
      841 agaaaatata ggtcaaagtg tcactatggt aatgggttct tatgaaaaca taaaaataac
      901 tagtccagaa gatttaaata tagcagaaca aatattaaat atggaaaaaa gagatgaagt
      961 taacagtaga aggagaatta tatgagaata ggattaggat atgatgtaca caaattaaca
     1021 gagaatagaa aacttataat cggtggtgta gagataccac acgataaagg tttgttgggt
     1081 cattctgatg ctgatgtatt gatacatgca ataatggatt ctattttagg tgctctagca
     1141 ttaggagata ttgggaagca tttcccagat acagatgaag aatataaggg agcagatagt
     1201 atgaaattat tagaacatgt gtataatcta ataacaagta aaggatataa aattggtaat
     1261 atagatagta ctataattgc tcaaagccct aagatggctc catatattga gagtatgaga
     1321 agtaatatat caaaagtttt aaacacagac attgataata taaacataaa ggcaacaaca
     1381 gaagaaggtc ttggttttac aggagcaaaa cagggaatag cttcacaaag tatttgttta
     1441 ttattgttga caagtcagaa taattaatat attattagta ataagtttaa tggatatcaa
     1501 gtaataggcc tatatagaat atctataatg tatgaattta agtgaaatta atataaatat
     1561 aaaattagct atgataaaga aatagtaaaa tgatgaaatc ttcacagaga gaaaacggtg
     1621 gtgagagttt tcaagaggaa gtctttgaac ctgtctttta gcttaaaggt ataaaaatct
     1681 ttacggtagg aaccgttata ttcccaaaag agagctattc atatagctaa ctagagtggt
     1741 accgcggaaa ttaaaccttt cgtctctata cttgtataga gatgagaggt tttttatatt
     1801 ttcaggaagt taatattcag gaggaatcta ttaatgaaaa tgtcaaaaat gtttatgccg
     1861 acattaaaag aaatcccagc agatgctgag attacaagcc atcaattaat ggttaggtca
     1921 ggcatgataa aaaaaatgac ttcaggtgtt tataaccaat taccaatggg gttaagagtg
     1981 ttcaaaaaaa ttgaacaaat aattagagaa gaattaaata aaaaagattg tcaagaaata
     2041 ctttgtgcag ccttacttcc ttctgaatta tggaaagagt ctggaaggtg gactgctatg
     2101 ggtgaagaaa tgtttagact aaaagacaga acagaaagag agtactgtct tgggccaaca
     2161 catgaagaag cattcactga tataataaga caagaaatta cttcatataa acaattgcca
     2221 ctgaacttgt accaaataca agtaaaatat agagacgaga gaagaccaag atttggtgtt
     2281 atgagaacta agacttttac aatgaaggat gcatatagtt ttgatgtaga tgataaaggt
     2341 ttagataagt catatcaaga tatgtttgat gcatatgtaa gtatatttga tagatgtgga
     2401 ttggagaata gccctgtaca ggcagattca ggagctatag gaggttctac atcagccgaa
     2461 tttatggtta agtcagaagt tggtgaagat gaagttgtat tttgtagtgg atgtgattat
     2521 gcagccaatg tagaaagagc agaatcttgt aatctagcat cacaaaaaga agaaatgaaa
     2581 gaattagaag aggttcatac accaggagca gctacaataa aagagttaga agaattttta
     2641 aaaacttctc cagataagtt tgctaaaact ttagtatatg aggcagatgg aaaaacagtt
     2701 gtagtcgttg taagaggaga tagagaagtt aatgaaatca aagtatctaa tgctataggt
     2761 tctgttatag aatttgctct tgctacagat gatgtagtga gaaaagtaac taatgcagaa
     2821 gtaggatttg caggtccaat aggtataaat gctgactatg tatttataga taaagaaatt
     2881 gttgaacaaa gaaacatagt tgttggggct aataaaactg agtaccatat aaaaaatgct
     2941 aactatggaa gagattttga aggaatagtt ggagatttta gaaatgttca agaaggcgac
     3001 cagtgtatag tatgtggtaa gccacttgaa atagctagag gtgttgaagt aggacatata
     3061 tttaaactag gaactaagta ctcagaatct atgaatgcaa acttcataga caaagatgga
     3121 aaatcaaagc ctatagtaat gggatgttat ggaataggtg ttgaaagaac agcagcagct
     3181 ataattgaac aacataatga tgagaaaggt ataatatggc ctttgtcagt agctccatac
     3241 catgttgtga tagttccagc aaatatgaaa aatgaagaac aaatatcaat agctgaaaat
     3301 atatataatg atttgcaagc tatgggtgta gaagttttat tagatgacag agatgaaaga
     3361 ataggtgtca aattcaatga ttcagaatta ataggaatac ctatgaggat tacagtaggt
     3421 aaaaatatta atgaaggtaa agtagaattt aaacttagac ataaagaaga taaagagata
     3481 attgacatag aagaaataaa tgaaaaagta aaggcagagt ttatcaggaa taatgttagg
     3541 ttaggataat aacttaaata agatattgta tagataatca aagttgtact ttatagacac
     3601 cattttatct atatataagg atataaaatg gtgttttttt ataaaacaaa taaatgagga
     3661 ggtaattgaa atggcaaaaa atgaaaaaca atttgttgaa gaaataacaa agatggaaga
     3721 tgattttcca caatggtaca cagatgtaat aactaagact gacttagttg attatgcacc
     3781 agtaaaaggt tttatggttg taaagccata tggatatgca ttatgggaaa agatgcaaga
     3841 atttatggat aaaaaattta aagaaactgg acataaaaac tgttattttc cattattaat
     3901 accagagagt ttattaaaca aagaagcaga acacgttgaa ggttttgctc cagaagttgc
     3961 atgggtaaca catggaggaa ataaaaaatt agaagaaaga ttatgtgtaa gacctacttc
     4021 agagactata atatgtacaa tgtatgcgaa atggttaaaa tcatatagag aattaccata
     4081 tctttataat caatggtgct cagttgttag atgggaaaaa tcaactagac ctttcttaag
     4141 aacatctgag tttttatggc aagaaggtca tacattacat gaaacagcag aagaagcaca
     4201 agaagaaaca atacaacaat tagaagtata taaagcgtta tgtgaagaat tacttgcaat
     4261 gccagttgta gctggtcaaa aaagtgaaag tgagaaattt gcaggtggtg aaagaacata
     4321 tactatagaa gctatgatgc atgatggtaa agcattacaa tcagggacaa gtcacttcct
     4381 aggtcaacac tttactaagg cttttgatat tacttttgca gatagagaag gaaatttagc
     4441 taatccatac catacatctt ggggagcatc tacaagactt ataggtggac ttataatgac
     4501 tcatagtgat aacagaggtt tagtattacc accaagagta gctccaattc aagttgttat
     4561 agttcctata gctgctaaaa aaggaaatgt aatggagact gttgataaaa tatatgctga
     4621 tttaaaaact aagggagtag ccgtagaagt tgatgataga gataactata caactggatg
     4681 gaaatttaat gagtgggaaa tgaaaggtgc tccagtaaga gttgaaattg gaccaaaaga
     4741 tatagaaaat aatcaagcta tggtatttag aagagatact ttagaaaagg attctatgcc
     4801 tttagaagga ttagctgatg ctatatgtga tttatttgat gttatacaca atgatatgtt
     4861 tgaaaaagca agaaaacata gagaagacaa tacatctata gttgaaaata tggatgagtt
     4921 tagaaaagct ttagaagaaa agccaggatt cataaagact atgtggtgtg gagatgcaga
     4981 atgtgaagct aagattaagg aagaaacagg agcaactata agatgcttac cttttgaaca
     5041 agaaaactta ggtcacaagt gtgtgtactg tggaaaagaa gcagatagta tggtagtaat
     5101 ggcaaaagct tactaaaata catttaaata aaaactgggg tagaaagtat ttaatacttt
     5161 atcccagttt ttttgttttt tatgagtaat aaaatgtatt tgtaactatg tcataagata
     5221 agtcatagat acttatatta aaaagaatat aatagagaac tatttttaat aattacatat
     5281 taatgaaagc aaaatatttt tgttatatag attttcaata aagcaaacaa taaatataaa
     5341 tgaatattag taaggagtgc ttttaatttt tagaaaataa atgacttaaa agaaagattt
     5401 atgaaattaa aactaggatt ttctgatata ataaacatgt gtatttttaa ataaaaagga
     5461 tattattatt agatttgatt aaaaataaac ttgcaaaaca atgtactagt ttaatattat
     5521 agttatattg aataaaatat caggaggaag taaaaatgaa tgtaagagta aggtttgcac
     5581 caagtccaac aggatttgta catataggta gtttgagaac tgctttatat aactatttat
     5641 ttgcaaaaaa aatgggtgga gaatatatat taagagtgga agatacagac caaagtagat
     5701 tagtagaagg tgctatagag aatatgctta atgctatgaa atgggctggt gtcaaccata
     5761 gtgaaggtgt tatattagat gatagtggaa aagtagtaca aaaaggagaa tatggtccat
     5821 atattcaatc tcaaagatta gatatatacc aagaatatat aaagcaactt ttagatagtg
     5881 gaaaagctta ttattgtttc tgtacaaaag aaagactgga tgaagtaaga gatgctcaaa
     5941 gagcagcagg agaaacagct aaatatgatg gtcattgtaa gaatctttct aaagaagaag
     6001 tagaagcgaa tataaaagca ggaataccat atgttataag attaagattg ccagagaatc
     6061 atacaattaa gtttacagac ttagttagag gagatatgga atttaatact aatgacctag
     6121 atgaccaagt attaatgaaa acagatggat ttccaactta tcattttgca gtagttgttg
     6181 atgactattt gatgaaaatt acacatgtta taagaggaga agaatgggta tcatctactc
     6241 caaaacatgt ttatttatat gaagcttttg gatgggaagc acctgtattt gtgcatttac
     6301 caaatatact taataaagaa aagaaaaaat taagtaaaag acaaggtgat gtagctgttg
     6361 aagactttaa gaaaaaagga tatttaccag agggattagt aaactatgta gcattagttg
     6421 gttggtctcc tgaagataat caagaattgt ttacaatgga ggaattagaa aaagccttct
     6481 ctgtagaaag agtatctaaa agtggtggag tatttgatac tgaaaaatta aattgggtta
     6541 atcagcacta tataaaagat ggtgatgatg cttatctaac agacttagca ataccatttt
     6601 tagtagaaga tggatttata acagaagaag aagctactaa taaatatgat ttcttaaaga
     6661 gtatgatatc agtacttaaa gagaaattac aatatgtaaa agaaattact gaacatgcaa
     6721 gtatattttt tggagataaa atagaggtag aaactgaaga gggaagagag ttcttaagat
     6781 tagaacatat acctacttta atagatgctt tgagagaaaa aatagaaaaa acagaggtat
     6841 taaatgctga gtttgttcaa gctatgctta aagaaataca aaaagagtac aaaataaaag
     6901 ggaaaaattt atttatgggc tcaagaataa ttttgacagg gcaaatgcat ggtccagatt
     6961 taccaaaagt aatggaagtt ttaggaaaag aaacttgctt aaatagaata gcttatgtaa
     7021 aaaataatat attatagtca taaaagtccg ctaatttaga agcggacttt ttttgtgtta
     7081 gtatttggtg aaaatatctg cttaagaaaa tgaaggtatt taaggtaata aatttaatat
     7141 ataaaaagca aagcaaaaat ataaataaaa tatgaataat aaactaataa aatattaagc
     7201 atagaagaag ggaatctatt attttagttt gatttaatta ttaagggggt aattactatg
     7261 tatagtaata ttgatgatgt taaaaaggaa ttaaaggagc tatgcttaga atatgttaca
     7321 attttagaaa aattaaaaga tgaaaaaatg attacagaag aaacttttga aaagtgctct
     7381 tctcaaaaaa aaatattttt agaagaacaa tagaggatgg taatagtatt attattatgt
     7441 agtttgaaag atagtacgaa aaatgatagt aatactatta tttattgcaa ttttcaacta
     7501 taatataata tataatgtta agaagataat ttataaaaat acagtgaggt gatgagtgtg
     7561 aaagtttata atacattgac tagaacaaaa gaagaatttg ttccattaga agaaggaaag
     7621 gtaaaaatgt atgtgtgtgg tccaacagtt tataactata tacacatagg taatgcaaga
     7681 ccatttataa tatttgatac tctaagaagg tatttagagt atagaggata cgatgtaact
     7741 tatgtacaaa actttacaga tgttgatgat aagattataa atagaagtca tgaggaggga
     7801 atatctcctg aagaagttgc ggctaagtat ataaaagaat attttgtaga ctgtgatggg
     7861 ctagggataa aaagagctac tgttcatcct caggttacag ataatataca acagattata
     7921 gagtttataa aagagttgga agataaggga tatgcatatg cagtaaatgg tgatgtatat
     7981 tttgatacaa ataaatttga aggttatgga aaactttcag gtcaaaaaca agaagattta
     8041 gaggcagggg ctagaattga ggttaatgac caaaaaagac atcctatgga ctttgttctt
     8101 tggaaggcaa aaaaagaagg tgagccagga tgggatagcc cttggggaga aggtagacca
     8161 ggatggcata tagaatgttc tgttatgtct aagagatatt taggtgaaac aattgatata
     8221 catgcaggtg ggcaagattt aacattccca catcatgaaa atgaaatagc tcaaagtgaa
     8281 gctagaagtg gaaaaatatt ctcaaaatac tggatgcata atggatatat aaatataaat
     8341 gatgaaaaga tgagtaaatc aaaaggtaat ttctttacag taagagatat atcaaaatta
     8401 tatgatttag agatagtaag atttttcatg ttatcagcac attatagaaa tccagtaaac
     8461 tttagtgatg agatgttaaa tcaagctaaa gcaggtcttg aaagactata taatactaaa
     8521 gaaaaattag aatttacgct aagtaattta gtagaatctc ctttaacaga aaaagaagta
     8581 gagcttgtta aagaattaga tgactttaga caaaaattca tagatgctat ggatgatgat
     8641 gttaatacag cagacgcagt aagtgtaata tttgaattag ctaagttaat aaattctaat
     8701 gtagatgaaa attcttcttt ggagtttgct aaaaaatgct tagatgaatt taatgaactt
     8761 acaggagtat taaatattgt aaataagaaa aaggatactg tattagataa agacattgaa
     8821 gaattgatac aaaaaagaac tgatgcaaag aaaaataaag agtttcaatt agctgatgat
     8881 attagacaac aattactaga tatgggaatt gtattagaag acacaagaca aggtgttaag
     8941 tggaaaagga tataaaaatg gaaaaaactg aattagttac tatgtcacca ttagttttag
     9001 catacttagg tgatacagtg tatgagacat atataagaga atatttaatt agacagaata
     9061 cacaaagaaa agttaatgat ttgcataaac tagctataaa gtatgtaaaa gctaaagctc
     9121 aggcaactat aattcatgaa attgaaattg agttaactga agaagagagt aaaatttata
     9181 aaaggggaag aaatcaaaag tcaaatactt ctcctaagaa tgcagatatt atagactaca
     9241 aacatgcaac tgggtttgag gcattagtgg gatatttgta tttaaataat gaaatagaga
     9301 gacttcaata tattatcaat aaaggaatca aaattattga aagagatatg taaatattaa
     9361 agagtgttgt cttaataata agtaataagg caacactttt tttataacta atgtataata
     9421 aatatataga aattagtaat gagaaaggat aagaaagtta tgaaagtaaa attaatatca
     9481 catacacctg aaccagaaaa agtaatagct atggcagcta agctatgtta ttcaccagtt
     9541 gggacagacg aaatagaaaa agatttctaa aatatattaa agagcattcc tataacaaat
     9601 actatggcgg ttggaatcta tgagaagtta tagatataga attatgtaaa ttatttggaa
     9661 aaaacaggtt tgaaaattag ccatgttggt atttctaggt tacttaagga agatcaaaca
     9721 taaataagct gagttgtctt aggtgcacta tgtacaacat tagaatgtga agttgaagat
     9781 ttattacttc aaacgaacga tggtactaag aaaaacaatg atgaaatgat taattaataa
     9841 taaaggtaag gaggtagtat atgagtttag cagataaaat ttttattgat atgtgtaata
     9901 atattcttga aaatggagta tcatcagatg gagaggtagt tagagcaaaa tgggatgatg
     9961 gccagcctgc tcatacaata aagaagtttt gtgtagtaaa taggtatgat ctttcaaaag
    10021 aattcccaat acttacacta agaccaacaa acttaaaagc agcaatagat gaattacttt
    10081 ggatatggca aaaaaaatct aataatataa atgatttgaa tagccgtatt tgggatagct
    10141 gggctgatga aacaggttca atagggaaag cttatggata ccaaattgga cagaaacata
    10201 aatacaaaga aggagaattt gaccaaatag atagagtgat ttatgacttg aaatataatc
    10261 cttatagtag gagaattata actaatatat ataatcataa tgatttaaat gaaatgaatt
    10321 tatatccttg tgcatatagt atgacattta atgtaactgg aaatagattg aatgcaatat
    10381 taaatcaaag atcaaatgat attttggtag ctaataattg gaatgtaact cagtatgcaa
    10441 ttttagttca catgttagca caaataagtg atttggaagt aggcgaattg gttcatgtta
    10501 tagcggatgc acatatatat gatagacata ttcctctagt taaagagctt attaatagag
    10561 aatcttatga tgcaccaaaa ttaataataa atccaaacat aaagaatttc tatgacttta
    10621 gagttgagga ctttacatta gaaaattata aaagtgggcc acaaatcaaa aagattcctg
    10681 tggcaattta gaaggagtta tttatgaaag ttatagtagc tgtagattta aattggggaa
    10741 ttggttatgg tggaaagtta ctacagtcga ttcctgaaga tatgaaattt tttaaagaaa
    10801 aaactgtagg taatgtagtt gtaatgggga gagaaacctt taaatcatta ccaggaaaaa
    10861 aagcattaaa agatagagtg aatatagtct tgactagaag taaatctttt aatgatgata
    10921 gattgattgt ttgtaattct atagatgaaa ctcttaagga actacaaaaa tatgaaaatg
    10981 ataaaatttt tataataggt ggagaaacag tatataaaca atttttacct tattgtaatg
    11041 aactatatgt aacaaaaata cagaagacat atgaggcgga tagatttttc cctaatatag
    11101 atattatgtc aaactgggat ttgattaaag aaagtgaagt aaaagaatat aacaatataa
    11161 gatatgtttt ttcaacatat ataaataata gtttataggt ttgtatagga gagaaattta
    11221 atttatgaaa aaggatatat ttgtaaatag agtttctaat gctttagaaa atgatagagg
    11281 cgcagttttt gtaggtagtg gtatatcgtt tagttcgact gaagttgatt ggtttaaatt
    11341 actggagcca ttagtaaatg aacttgatat aacattagac aataaaagtg atgatttacc
    11401 tatgattgct cagtacatag taaataatta ttcaggaaat agagggcctt tagtaaacga
    11461 aatatcaaaa acatttaata gaaagtttaa actaaacaag tatcataagt atttagcaac
    11521 aactaaactt tcaacaatat ggacaactaa ttatgatatg ttgttagaag atgcatttta
    11581 tgattttgat acatcggtta aagtaagtga tgattctatt acaaggaata gactagaata
    11641 tgatgtggaa attataaaaa tgcatggatg tatttctaga tccgattgga atgaaatagt
    11701 tataactcaa gaagattatg atgatttttc aataaataaa ccagctatat ctcaaagatt
    11761 aagtgaagat ttaataaata agtcttttct atttattgga tatagctata gagatccaaa
    11821 tataagaaat ataatgataa atgctagaag gctaagtaaa aaacatactc aacaacatta
    11881 tataatttta aaaaagttaa aagatagtga tagtaatatt acgcaagatg atgtagtaag
    11941 acaagaactt tggtgcaatg atttaaaacg tttgggaatt tcaactttat ttatagatga
    12001 atactcagag ttagaggaac ttttaagaga gataagtcaa aaatcaagag gtaaaacagt
    12061 atatgtgaca ggttctcata aagaaagcaa tatgaatgca agaaaattag gaaccaagct
    12121 agcaaaacat aaagatatta ctttactaag tggtcaatca tctggtattg gctcagaggt
    12181 agttgctgcg tttactgaag agtgtattaa gagtaaagaa gatattataa atagaatgaa
    12241 aatatttcct aatccatatt cagctgatga aaaatactct gatgatatta ctttaatacc
    12301 agatttaaag aaatgtagaa ctaaaatgtt gaattcaaca caagttgtag ttgtatttag
    12361 tggaggtata ggtactgaag cagagataga tgttgctata aaacaaaact gtaatataat
    12421 tcctgttata acatgtaaag aggatagaga gaatccagct ataaaaaaaa tcttaaacta
    12481 tagtgataat atgaaaaata tagaaataaa agatgctacc tattataata aaatagtaga
    12541 tgaagatggt gttgtaacaa tggaggaaat ttatatatgc atatcaaaaa taatgaatta
    12601 aaacatgaca ttattaaatt gaaaaaaaga aatggattta ttgaagttgg aaaagataaa
    12661 aaaacgttgg taaactgttt aattggagcg aatgaattag aggattacaa atatgaaata
    12721 aaaaaaatag attgtatact taatatatca gaaaaaccag atatagtttc agatttaagt
    12781 actaaaagaa gaaaaataag tgaatctata tggtataaga tagttaaaga tacacctttc
    12841 gtatctgcta cactgcccat atacttagtt agtaaaaata ataataaaat agatagtaat
    12901 gaattattag atataattat tgagcagatg gaaaatggag ttgggatgat aacgattcat
    12961 ccaacagcaa ataaacaaat ttttgaggat tctaagaata gaatggtacc tgtcacatct
    13021 agaggtggag gtatggtaat acaagattta atctcaaaaa atttttcaga agataatgtg
    13081 tatttgaaaa tattgccaga aattataaag tatgctcaaa aacataatgt ttgtatcagt
    13141 ctaggtgcaa catttagatc aggaaacata tttgattcta atgatagagc acagataaca
    13201 gaaattaaga gtcaaataga attagctaag aggatatata aaagtaatgt cggggttatt
    13261 attgaatcac caggtcattc aagaccaagt gacataatta aaatatcgtc aatattaaaa
    13321 aaagaaggtt tcccaattat gccactagga ccaataccaa cagatattgc aataggaatg
    13381 gatcatatcg ctgcagctat tggtgctaca attatgggta tagaaggatg tgcaaatata
    13441 atatcaactg tcacacgaga agaacatact ggagggagac cttctattga atccacagtt
    13501 gaatctataa gaacagcaaa aatagctgct catattattg atatacatca aatgaatgat
    13561 attaacttag atatgagtgt atctaagtct agagctaaat ctaacacctg tatatttggg
    13621 aaagatacaa gatattgtga cagatgtaga gaactgtgtc ctttgtgcat tagataaaaa
    13681 ttatattcaa gttaattcaa taaaaaagat tggataaaaa tacatcgtat agctagtttt
    13741 tcacaacaga gtcaaagata tgtcaaatta gagcaatttg aatacataat tcctccagaa
    13801 atagaaaaaa tagaaaaagc aaaagagtta tttataaatt caatgaaaaa ataccaagag
    13861 gattatgata aattagtaga gatactattt gaaaatcatt ataacaaact aataaaaggt
    13921 ggtaaaaacg agaaaacatc aaaaagacaa gttgagaaga aagcaataga agatgctaga
    13981 tacgtatttc caaatgcgtg tgaaacaaag atggtattta ctataaatgc tagaagtttg
    14041 tttaatttct ttgaacatag atgttatgaa aatgggagtt gtctagaagg aactatgact
    14101 tgtggagata tagttcaagt tagaaaaaaa tttaagaact tgtaggaggt gaataatttg
    14161 gcgagtatag aaggaagaaa tccggtaata gaagcaataa aaagtgacag agaaatagat
    14221 aaaatattaa ttgcaaattc agctaaagaa ggttcaatta agaaaataat aggaatggct
    14281 aaagataaga atataattat tcagtatgtt gataaacata agctagatga agtaagtaca
    14341 agtcattcac atcaaggtgt aatagcttat gcaagtgagt ataagtatta tgaattagat
    14401 gagctaatag atttagcaaa gaataaagat gaagacccat ttttcattat attagatgag
    14461 ataacagacc cacataattt gggttcaata ataaggacag cagatgcagt aggagcacat
    14521 ggagttatca ttccaaaaag aaggtctgta cacataactc ctgtagttgc caaagcatct
    14581 gctggagcag tagaatatat gccagtttgc aaggtaacca atatagttaa tacaataaaa
    14641 agactcaaag aagaagggct gtggattgct gcagctgata tggatggaga aactttttat
    14701 aaacaaaatc ttactggtcc tcttggtgta gtaattggaa gtgaaggttt tggaatatct
    14761 agattggtta aacaaaactg tgactttata gtaaaaatgc caatgatagg taatgttaca
    14821 tctttaaatg catctgtagc aggtggaata cttttatatg agatattcag acaaagactg
    14881 gataaaagta aataggcatg aaaaaaaata taaatcatta cttaataata gatggctaca
    14941 atattataaa tgcgtgggac aatttgaaag aactggctaa agaggactta gaagattctc
    15001 gagaaaagct tattgatgac attatagaat tttctgagtt tatgggttat aagactataa
    15061 tagtttttga tgcttataat gttaaaaatt ctcgagaaaa agttgaaaaa agaaaacaca
    15121 taacaatagt ttatacaaga gaacaccaaa cagcagatag ttatatagag aaatttataa
    15181 cttctttatc aaagtatgat gatgtaaaag tagccactaa tgactatgca gaacagcaaa
    15241 tgattttagg aaaaggtgct actagaatgt ctgcaagaga attaaagtta gagttagatc
    15301 gttccaagaa taaaatgaag gaaaaaaata tcggtttgag gaaaaaaatt cagcgaaatt
    15361 ggttggagga aaggttagat aaagaaacat tgtcgaaact tgagaacatt cgtagaaagc
    15421 gttgaaatta ctaggttctt tagactataa tatacctata aatttgtttt atatatgggg
    15481 gagatttaca tgttggtagc aaaagaaaaa agttatgagt tagtggacaa ttgtcagcag
    15541 gatgagtata acattgtatt aagagcaagt gaaggggata aaatagcact agagtacatt
    15601 attactaaat acagaaattt tgtaaaggca aaagcaaagt catatttttt aattggagca
    15661 gataaagaag atataataca agaaggaatg ataggccttt ataaggcagt tagagatttt
    15721 gatggaagta agaccaattc atttaaatgt tttgcggaga tatgtataac aagacaaata
    15781 ataactgcta taaaaactgc aacaagacaa aaacatatac ctttaaattc atatgtttct
    15841 ttgaataaac caatatatga tgaagagtca gacagaacac ttttagatat tatagctaca
    15901 agtatagtaa cagatccaga agagcttata attagtaaag aagagttaaa aaatatagaa
    15961 tcaaaaatga acgaattgtt aagtgacttg gaattagaag tactagaatt atatttaaat
    16021 ggaaagtcat accagtttat agcggacaag cttaaaagag atgtaaagtc aatagataac
    16081 gctttacaaa gggttaagag aaaattagaa aaacatcttg aaaacagaaa tgattaatag
    16141 taaaatactt accataagct tattgtgagc aaaattaatt tatatcgaaa gataagggag
    16201 gaaattcaaa atggctaaag ctaaatacga aagaacaaaa cctcatgtta atatagggac
    16261 aataggacac gtagaccacg gtaaaactac attaacagca gcaataacaa aaacattata
    16321 tgacagatat caattaggag aagcagtaga tttcgcaaac atagataaag ctccagaaga
    16381 aagagaaaga ggaatcacaa tatcaacagc acacgttgag tatgaaacac caaatagaca
    16441 ctatgcacac gttgactgcc caggacatgc tgactacgtt aagaacatga taacaggagc
    16501 agcacaaatg gacggagcaa tattagtttg ttcagcaaca gatggaccaa tgccacaaac
    16561 aagagagcat atactattat caagacaagt tggagtacca ta
//
LOCUS       DACTGW010000070        16525 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.070, whole genome shotgun sequence.
ACCESSION   DACTGW010000070 DACTGW010000000
VERSION     DACTGW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16525
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.070"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            167..2479
                     /locus_tag="I9573_001791"
     CDS             167..2479
                     /locus_tag="I9573_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAT4796414.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKENNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDTTSNKVNYISDELD
                     TTSNKVNYVSDELDTTSNKVNYVSDELGMALHKVNCVSDELDMTSNKVNYVLDELDIT
                     SNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIKFKVCF
                     DMFMTDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFME
                     LAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNLVAWEDKKFET
                     PSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKK
                     SIAQAYINQRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHG
                     NPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            2567..2902
                     /gene="spoIIAA"
                     /locus_tag="I9573_001792"
     CDS             2567..2902
                     /gene="spoIIAA"
                     /locus_tag="I9573_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HAT4796415.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            2916..3344
                     /locus_tag="I9573_001793"
     CDS             2916..3344
                     /locus_tag="I9573_001793"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HAT4796416.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3367..4131
                     /gene="sigF"
                     /locus_tag="I9573_001794"
     CDS             3367..4131
                     /gene="sigF"
                     /locus_tag="I9573_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HAT4796417.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            4411..4848
                     /gene="spoVAC"
                     /locus_tag="I9573_001795"
     CDS             4411..4848
                     /gene="spoVAC"
                     /locus_tag="I9573_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HAT4796418.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            5002..6015
                     /gene="spoVAD"
                     /locus_tag="I9573_001796"
     CDS             5002..6015
                     /gene="spoVAD"
                     /locus_tag="I9573_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HAT4796419.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            6029..6391
                     /gene="spoVAE"
                     /locus_tag="I9573_001797"
     CDS             6029..6391
                     /gene="spoVAE"
                     /locus_tag="I9573_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HAT4796420.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            6574..7590
                     /locus_tag="I9573_001798"
     CDS             6574..7590
                     /locus_tag="I9573_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAT4796421.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFVGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDRSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            7965..8807
                     /locus_tag="I9573_001799"
     CDS             7965..8807
                     /locus_tag="I9573_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HAT4796422.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            8822..9286
                     /locus_tag="I9573_001800"
     CDS             8822..9286
                     /locus_tag="I9573_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796423.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIITCFGQMIALKTQAQDLLIGSISG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            9300..10445
                     /locus_tag="I9573_001801"
     CDS             9300..10445
                     /locus_tag="I9573_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAT4796424.1"
                     /translation="MNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPELAFEEYKSQ
                     KALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPGMGHACGHNI
                     IGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFNNVDAALILH
                     PSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSVNSMRLHLKD
                     YARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKGAAISTGTNV
                     EIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGNLTHEIPAIQ
                     AYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            10489..11424
                     /locus_tag="I9573_001802"
     CDS             10489..11424
                     /locus_tag="I9573_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HAT4796425.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(12223..12621)
                     /locus_tag="I9573_001803"
     CDS             complement(12223..12621)
                     /locus_tag="I9573_001803"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAT4796426.1"
                     /translation="MKWKELYNNARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            complement(12920..15613)
                     /locus_tag="I9573_001804"
     CDS             complement(12920..15613)
                     /locus_tag="I9573_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAT4796427.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQST
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTTEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANQG
                     VYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            15827..16525
                     /gene="yunB"
                     /locus_tag="I9573_001805"
     CDS             15827..16525
                     /gene="yunB"
                     /locus_tag="I9573_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HAT4796428.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
BASE COUNT         6086 a         2147 c         2960 g         5332 t
ORIGIN      
        1 ttagtatgca aaattctgtg aagcaggaac actgtgactt tagtcatggg aggttcagaa
       61 aatatagcta tgttttatat actgtagttt tgaacataga aagtttaata aattatagta
      121 tatattaatt tttaataagg taagaaagta gaaagaagga tattttatgg atataataaa
      181 aaaattaagt catggatgta cattagattg tcatgattgt tgtaaattca atgtttatac
      241 taaagaaaat aatgtagtaa aaattgaagg agataaaaat catccttata caaaaggttt
      301 tatatgcaaa aaagggatgg ctcatttaga tagattaaat cataaagata gaataaagac
      361 tcctatgctt aaagttgatg gagtatggaa agagatttct tttgataagg ctatagaaat
      421 aatggcagaa aagcttacat actataaaga aaagtataca tcaaagtctg tcatgcatta
      481 tgaccaatat ggaagtgggt ctgtgctaaa atacatagga gatatatttt ttaattttta
      541 tggaggtgtt agcagacata agggtggtcc ctgttggagt gcagggatgc atgctcaaaa
      601 atatgatttt ggagtagcta agagtcatgc tatagaagat atgttaaata gtaaaagtat
      661 atttgtgtgg ggaaaaaatc cagcatatac tacaattcat actatgcaaa taattaaaaa
      721 agctaaagaa aaagggataa agatagtagt gattgacccc atatatacaa aaacagctca
      781 aatagcagat aagtatgtac aggtaaatcc tggaacagat ggagctttag ctatagctat
      841 ggcaaaaatt atagtagaag ataaattata tgatgaagaa tatataaact cgtatgttat
      901 tggttttgaa gaatataaaa aatatttatc ttcattggaa ttaagttttt taattgatga
      961 atgtggtgtt aaagagaatg atataagaga gttagtggat ttatatacta acaaatattc
     1021 aagtataaat gttggatatg ggcttcaaaa atataaaaat ggtggaaaca caataagagc
     1081 gatagatgct ttaggtgcta taactggtca aataggattt agtggtggag gggtaaacta
     1141 tgctaataaa gtttatccaa gtgttataaa ttctgaccct tacaatagcc aatcatatgg
     1201 tgaagatagg gagttttatg taagtaatat cagtaaattt atagaagaat ctttaaaaaa
     1261 tacatcaaat aaagtaaatt atgcatcaga tgaacttgat acaacatcaa ataaagtaaa
     1321 ttacatatca gatgaacttg atacaacatc aaataaagta aattatgtat cagatgaact
     1381 tgatacaaca tcaaataaag taaactatgt atcagatgaa cttggtatgg cattacataa
     1441 agtaaattgt gtatcagatg aacttgatat gacatcaaat aaagtaaatt atgtattaga
     1501 tgaacttgat ataacatcaa ataaaacaga ttatatatca aatgaattgt ataatttatc
     1561 taataagtca atcaaagata atattcctat aaagatggct gtgataacta aaagtaatat
     1621 gctgaatcaa ctgccagatt tagtagaatt ggagagggtg ttttcaaaaa ttaaatttaa
     1681 agtatgtttt gatatgttta tgactgatac agctacttta tgtgacatgt ttataccgtg
     1741 tacaaataca ctagagagtg aagatataat ttatagctca atgaccaatc catatataac
     1801 ctataatgaa agagctgtaa agccagcaca taaattgatg gatgaatatt atttctttat
     1861 ggagctagct aaaaaaatgg gattaaatga ttatcctttt gtagaaaaga gaacttattt
     1921 agaaaaagtg attgaaccac ttaagcgttt tgacaaaaat ctggatatag aaaaacttaa
     1981 aaacaattac tttactatac ataaccttgt tgcttgggaa gataagaagt ttgagactcc
     2041 atctggaaaa tacgagcttt attcagaaag tataaaaaac ttgggaataa gtccaacacc
     2101 tgtatatata agcaataaat ataaagaaat agaagataaa aatatttctt ttagattatt
     2161 aactaatcat catgcagata ctttatttag tcagcatttt atggacaaaa aatctatagc
     2221 tcaagcatat ataaatcaga gaatggctaa aaaagtaggt attgaggaca aagatattgt
     2281 gatattgagg tcaaaaaagg caaaaataaa tgtacaaata aatatagatg atggagtagg
     2341 taattatata gtaaaaatgt atgttggttg gtggaagaaa catggaaatc ctaatagttt
     2401 aacagacact ggtatatcag attttggagg tcaggtaaca tataatgaaa gtatggtaga
     2461 aataatacga caaaactagt taaatttcat tgaaggaata aaaatataat tatagaattg
     2521 attaaaagtt actattttta caaaactatt gaaggaggga tttttgatgg taaattattc
     2581 cttagaacat aagaatctat acatagaatt tatgtgctca gaactagacc accatgtagc
     2641 caatgaaata agggaagaaa tagataactt attaagtgta aatcaagtaa aaaatgttgt
     2701 atttaatttt gaaaatatta attttatgga ttcttcaggt ataggcgtca tcataggtag
     2761 atataaaaag atttctaatg aagggggacg agtttcagta attaatataa gttcacgagt
     2821 taagaagatt tttgatttat ctgggttaaa taaaattatt ggcatttatg atacgtatga
     2881 agaagctcta agttctttgt agggaggagt atgtgatgaa taatattatg gaagttaaat
     2941 tttcggcaat atctgaaaat gaaagtttag caagagttat agtagcatcc tttgcagcta
     3001 agttagaccc aacagtagat gagttagtag atataaaaac tgctgtctct gaggctgtta
     3061 cgaatgctat aatacatgga tatgaagaag acagttctaa gtttgttttc ttaagatgtg
     3121 agatagaagg aaatactata aagattatag ttgaagatga agggtatgga atagaaaatg
     3181 tggagaaggc aatggaacca ctttatacat caaaaccaga acttgataga tcaggaatgg
     3241 gattcacagt aatgaaaagc tttatggacg atgtagaggt aagttctgta aaagataatg
     3301 ggactagaat tgaaatgact aaaaaaataa atgtgcctaa ataaagtcca aaagtgaggt
     3361 tttattatgg aagtaactgt tgccagagaa gaaaaaaaac ctcttcttag tcatgaagag
     3421 actctagaac ttattgaaaa agttcaaaat ggagatgaag aggcaaaaga aatattaata
     3481 tcaagcaatt taggtctagt taggagcgtt gtatctagat ttttaaatat aggatatgat
     3541 agagaagatt tatttcagtt aggttctata ggcttaataa aatcaatata taaatttgac
     3601 ccaaaattta atgtaaaatt ctctacttat gcagtaccaa tgattttagg tgaaataaag
     3661 agatacttaa gagatgatgg tatgataaaa gtatcaaggt cgttaaagca aatagctgta
     3721 aaggcaaaga tggaaagtga agctttaact aaaaaattag gtagagagcc aagtatagaa
     3781 gaattagcta aagcaataga tgtagaaaaa gaagatttag taatggctat ggaagctaat
     3841 ttcaatgttg agtatttgca aggagttatt catgaagagg aaggttctcc aatatgcctt
     3901 attgataaaa taagcatgaa aggagaaagt gaagaagaaa aagtagtgga taacatattg
     3961 ttaaaagaca tacttggaag actggataag agagagcgtc aaataatagt tctcagatac
     4021 tttgaagaca tgactcaaag tgaaatagga gagatgctca atatatcaca agttcaagta
     4081 tctaggatag agaagaaagt attgtctaag ttaaaagaat atatatcgtg ataatactta
     4141 tgatatgtag aataacaaaa tataataaat atattactaa aataaaatgt aataactttt
     4201 tatgttaagt ttttaaatta ttaaaagata ctaaaataat aaaaagcatt aaaaaagtaa
     4261 aaatataaaa acaaggtgtc tttatggaaa aatagatttt ttctttatta ggcatctttt
     4321 ttattttttg accaaacttt agatagtcaa aataaataat aggtttattt ttttaaaatt
     4381 aacaaataat actttagaga ggtgtttagt atggataaaa attataaaaa atatgtagac
     4441 caaataagcc caaaacctac atatttaaaa aactatacac ttgcatttat tgtaggtgga
     4501 attatttgta tgatagggca agcaataaat gatttgtata tgaaagttgg aggtcttgat
     4561 aaactaggtg ctagttctgc aacatcaata acattaatat ttataggggc atttttaact
     4621 ggattaggag tttacgacct aataggaaaa agagctggag ctggttctat aattccaata
     4681 acaggttttg ctaactcaat agtatctcca gcaatggagt ataaaagaga aggctatgta
     4741 ttaggtgtag gtgcaaactt atttaaaatt gcaggaccag ttttagttta tggtataggt
     4801 tcttctattt tatgtggaat tatttattat atttttaaga tgttttagat gatttttatt
     4861 taatttaaat tttattaatt atgaagaagg tataaaatct gaaaatagct actaaagtaa
     4921 ctaattgaaa aataattgtg cagatttaat taatattaaa aatttgaata gtaaaatgca
     4981 aaattaaaaa ggtgtgaaaa tatgaaaaat aaaagaattg gaaaaagaac agtcaagtta
     5041 gaaaataaac caaccataat atcaactgga actatagtgg gtcctaagga aggtgaagga
     5101 ccactaaagg actattttga tatgattatg actgacgatt tatatggtga aaagacttgg
     5161 gaattagctg aaagtaaaat ggtagagact gcatcccaac aagctatcca aaaggcaggt
     5221 aaaaaattgt cagatgtaaa ttatatgcta ggtggcgatt taataaatca aatagtgcca
     5281 gcatcatttg cagcaagaga actggctatt ccatttttag gtatatatgg agcttgttct
     5341 acaatggcag aaggtctttg tataggttct atgcttatag atggtggttt tgctgattta
     5401 gtactgtcag gtacatcaag tcactattgt acagcagaaa gacaatttag attcccactt
     5461 gaacttggaa atcaaaaacc aatgacagct cagtggacag ttacaggagc aggaagtgta
     5521 ttattagctc ctaatggaga cggtccaaaa gtaaagtatg ttactgttgg taaagtaata
     5581 gatgagggta ttgatgatgg taataatatg ggaccagcta tggctccagc agcaatagat
     5641 actatctact cttactttga agatactaat gatgacccaa atagctttga tataattgct
     5701 actggagatt taggtactct tggaaaacag atagcagaag actttctaaa agaaaaaggt
     5761 gtagatatat caaaagttta tactgactgt ggtatagaga tgtttaacct taaagaacaa
     5821 gatgtccact gtggtggaag tggatgtggg tgttcagcta cagtgtttgc aggatatata
     5881 tatgataagt tgagaaaaaa agaatttaac aaagtaatgc ttgtatcaac aggagcacta
     5941 ctttcaccga caagtacact acaaaagcag acaataccat gtgttgcaca tgcagtagta
     6001 atagtaaatg agtaggaagt gatataatat gttaatggac tatgtaagag tatttattgt
     6061 aggtggaatt atatgtttaa tagcccaagt aatgatggat tatttcaaaa tgcaaactcc
     6121 atatgttatg gtaacgtatg taacaactgg tgtagtttta acttttctag gactttatga
     6181 gccattagtt aagtttgcag cagcaggtgc aacagttcct ataataggat ttggatattc
     6241 acttgctaca ggagtaataa aatcaattca aagtgatggt tttttaggta tttttttagg
     6301 aggtacaact gctacagctg gtggaatagc agcagctata ttctttggat atattatgtc
     6361 agtagtattt actccaaaag caaaaccata aatctttatt aaaagaataa taagtttttg
     6421 aaataataac tttttataaa gcaggtggat atcttttaga ttccacctat tttttatata
     6481 aagaagcgtt tacataattg aagttgacaa ttatttacaa catagtgtag tattatatta
     6541 tcaaaataaa atgaagtgaa gtaggggata aagatggtaa ctattaaaga tattgcaagg
     6601 aatctaggta tcagttattc aactgtttct aggtgcttaa acaataatcc taatgtatct
     6661 gaaaaaacaa aaaagaaagt agtagaagag gcaaatagac tagggttcca ttttaatgta
     6721 aatgcaagaa atttagcaaa aaaagaaact aacaggatag gggtaatctt ttcaaataat
     6781 tttaatcatc aagatactag aaagtttttt agtgatataa tggatagttc tataaattct
     6841 attgagacta ataaatacga ttttataata cagcctaata ataacattag tggtgatagt
     6901 aatgtatata aaatggtaaa tggacaaatg gttgatggat tagtaattgt gtctcaatca
     6961 ataaaaaaag aagagtatga ttttttaaaa gacaataatt ttccacatgt atttatatat
     7021 tttaaaccat cttttgtagg agaagtagac aatttctttt gggacgataa cgtgtatgga
     7081 ggttatatag ctaccaagca tttaattgac catggtcata aagacataat aacagtaact
     7141 tcagatgata ggagttcaaa aatgcatgaa gatagaacta agggatattt aaatgctatg
     7201 gaagaggcaa atttaaagac cgaagtcatt aaatctaaaa tggattttga atctcaagtt
     7261 gaatttttag aaaaatatat tgataaaata aaaaaagctt cagctatatt tgtacaacaa
     7321 gatgtacctg cgatttctat aatacaagag ttaaaaacta cttatggtat aaatgtacca
     7381 gaagatattt ccataatagg gtataataat attgaattaa tatcatattt taggtcgcat
     7441 ttatcaacaa tagatgaccc aagagagcaa gttataaaaa atggagtaga tagtttagta
     7501 aatataataa ataaaaagag tacagaacac attccaagaa aattatatcc aaggttaata
     7561 atacgaaata gcgttaaaag aataaagtag taagctttta agtatcaaaa gtttattaaa
     7621 aatattatag attatgtgaa gttttttaac aaacaatata aataaaaatt aaaaggagta
     7681 ggtgtatata agtacattgt actcctttat ttattgagat agtttttata atatgaatga
     7741 ataattttat tcaattattt gaaaatccgt tattttaaca atataaatta ataaattatt
     7801 tgtattttat atcatgaatc tataatattt ttttgattca taactaaaag tatataaaaa
     7861 tgatgaataa atatatatta tacattatca atgataggtt atataataaa atcactaaca
     7921 attatattat aagaaaatta taaaaattac aaggaggtat agaaatgaaa gaaaaagcag
     7981 ggattagaaa tgccataata gcagccttta taatcacaat attggcagaa ttaattggac
     8041 ctatttcatt taaagttatg ggaataaatg taactttact tagtatacta tgggctatat
     8101 tcataggaat ggctgtttct cctcatttat tagggagagt tataccagcc ttaaaaaagt
     8161 ttatagggga taatgaaata aatgtatctc catatttatt atctttgaca ctatatccat
     8221 taggaataat gtttggaatt aatgcagggc ctaaagttgg tatattactt caagcaggac
     8281 cagcactttt attacaagaa tttggtcata tgggtacaat gttgatagca ttacctgttg
     8341 gagtagcatt aggtcttgga agaagtgcat taggtggtac tttttcatta tgtcgtgaca
     8401 cagcactagg cattattgga gataagtatg gattaaactc accagaaggt ataggaacac
     8461 taggaactta cataagtggt agtatatttg gaacattatt attttcattt ttagcaccag
     8521 tgggtgtact tttaggattt catccatatg ctttagcaat ggcatcagga atgggtagtg
     8581 gttcaatgat gggagctgca acagcatcat taatgaatac ggttccacaa atgtcagagc
     8641 agattttagc atattcagct actagtggat tgattacagc tgttactgga atatatatag
     8701 aattattctt atctttacca gtagccaact actattatag caaagtagct ccagttattg
     8761 aaagatttag aggaagaaaa acaagaagaa caagtaataa attgtaaagg aggatatata
     8821 tatggaaaaa acttttaaga ctattatgga gcagataaaa atagtattgt ttatatatat
     8881 tattacttgc tttggacaaa tgatagcact taagactcag gctcaagatt tgttgattgg
     8941 aagtatatct ggatttttgt tggtgataat tgctattata ataaagaatt ttgttaaaaa
     9001 acctaattta ccaggttttg catgggctac attagttgca ttcgttttat cgcttccaat
     9061 atctccagtg ggggatatca tagcaacaaa tgtagggaaa atcaatttta tgtctacagt
     9121 aacaccatta ttagcttttg caggaatctc tgtaggtaac caattagata tattaaagac
     9181 tttgagttgg aagttagtag ttatatcatt tatagttatg acatctactt actttggttc
     9241 tgcttttata tctcaaacag ttttaagatt aaatggaatg ctttaaggag gtagtaaata
     9301 tgaatgaatt aaaagcaaaa attaaagagg aaataaagtt attatctgaa gagaaaaaga
     9361 catcatttga aaaagtttct gactatttat tttcaaatcc agaattagca tttgaggagt
     9421 ataagtcaca aaaagcatta tgtgatttgt tagaagaaaa tgaatttaat gtaactaaag
     9481 gtgtaggagg attagaaaca tcttttgaag ctgtgtattc aaatggaact aatgggaaaa
     9541 cagttgcttt tctagctgaa tatgatgcac ttccaggaat gggacatgca tgtgggcaca
     9601 atatcatagg gacaagttct gttggtgcag gtatcatatt aaaagaaatc atgaaaaaac
     9661 ataatataga agggactgta aaggtttttg gaacacctgc agaagaaaga gtaggtggaa
     9721 aaataactat gattaaagaa ggtgtattta ataatgtaga tgctgcatta atcttacatc
     9781 caagtgatgc ttcaatgcct gatgatatat catttgctca agttaattta aaatttgatt
     9841 ttactggtaa ggcatcacat gctgctgctt ttccttggga agggaaaagt gccttaagtg
     9901 gagtaatagc actatttaac agtgtaaata gcatgagatt acatcttaaa gattatgcaa
     9961 gagtacatgg aattataact gatggaggta gcatacataa tataattcct gaaaaatcta
    10021 cagctatatt taatgtaaga gccttaagca tagaatattt aaatgagatt tgtgaaatgc
    10081 ttaaaaattg tgctaaagga gctgcaattt ctactggaac taatgtagaa atagttcaat
    10141 tagatgaaat atacaaagag attaagaatg attctgaatt agtgaatata gttagacaaa
    10201 attttgaagt tttgggtgaa gactatgttg aaagagattt aagtcaaggt ataggttcaa
    10261 ctgatacagg aaatttaact catgagatac ctgcaataca agcatatata aaattaaaag
    10321 aaaatactgc aactcataca gatgaatttg ctgttgcagc tggtggtgaa gaaggcaaag
    10381 tagcacttat aaaagcaatt aaggtgctag caatgtgtgg agtagatatt ttgtatagta
    10441 aataatatat aattttaaaa tatattaaga aaggaaatcg gattagacat gatattaaaa
    10501 caagtaatag aggtttatga cattttagat aaagcaaatg ctaatgggga agaagtaaaa
    10561 acttacttac aaggttatgg tgaagtagat gtaactgtaa aagaattgtc ttcaagtaaa
    10621 ggttcaacag atttagtaaa gcttacaatt cctgggaaaa atggaaaatt aaaaggtgga
    10681 gatgctccta ctcttggtat tttaggtaga ttaggaggga ttggagcaag accagaagtt
    10741 ataggttttg tctcagatgg tgatggagca ttggtagcaa ttgcagtggc tgctaaactt
    10801 ttggatatgc aacgaaaagg ggacatatta gatggtgatg ttgtaatttc aactcatatt
    10861 tgcccagatg ctccaactaa agaacactat cctactcctt ttatggattc tccaattgat
    10921 atgatgacta tgaatgaaaa tgaagttgat agtagttgtg atgcaatact ttctatagat
    10981 actactaaag gaaatagagt aatcaatact agaggatttg caatatcgcc aactgttaaa
    11041 gaaggctaca tattaaaaac tagtgataac cttttagata ttatgcagac tgtaacaggg
    11101 aaatctccat ttgtattccc tttgtctatt caagatataa caccatatgg aaacaatctg
    11161 tatcacttaa atagtatttt acaaccagca gtagctacag atgcaccaat agtaggagta
    11221 gcaattacaa cagagatgcc tgttgctgga tgtgctacag gtgcaactaa ttttagtgat
    11281 ttagattcag ctgggagatt tgcaattgaa gttgccaagc tatttggtag aaataagtgt
    11341 gatttctatg ataaagaaga gtgggaaatg cttattaaga gatatggcaa attaaataga
    11401 tttcaaacat ttggtataca gtaattagaa aaatattaag tttatataat ttgtgataca
    11461 actatttaag aagatataaa caattaggaa taaaagtaag aataaagcac ttagttaaaa
    11521 actttaaaaa aaaatattta tttgtttata taaaaaggag gattctggtt attgggattc
    11581 tcttttttat gaatacaaat aatatattgt aattataaaa aaaggagcgt ttcaaagtga
    11641 tttttttaat caccttgaga caactcctta tattttttat ttaaattcta ttttttattg
    11701 actgtatagc tattttcacc atcataaggt ggcatgacct tgaacagcgt ggttttgtaa
    11761 agttgggcac cacaaaacaa aaaatcaaca agcccttgtg tggtctgctg acgtgtttac
    11821 tatactatac tttcccaaaa accgcaagca ctatgggagg ctataaggcg gcgaccattc
    11881 aggggcgatc ccctcgaaac cgggataggg ttcccatgtg ggacataata aagagcgccc
    11941 cggaggtatc aactaccatc cggggcgctc tttattatgt cccgtgggaa ccatgcccac
    12001 gaagcagggc gtacaacgga cagataggtc ctaccctcct gacagactgg aatctcaatc
    12061 ccatcgaaaa caatcataac aatcatggca agattatata cactcctgta aaatattttt
    12121 gtgtgtacca tgaagtactg cttgccacta ttgagaacgg ataaaaggga agtacgtatt
    12181 ataccaataa ggtgaacgaa acaaaatact catttgataa agttaaataa acctgtcgga
    12241 accccaccaa tccggcaaga gctcactcaa tttaacagtt ttttctgttt cgacattaac
    12301 taaaatctca atacaagcac tatccttatc aagttgcatt aaatattcac gacaagcacc
    12361 acatggtgag ccaacactcc cattgctcat cacacaaact aatttagtaa ttttactttc
    12421 gccatttgtt atcatattgg cgatagcatt tctttctgca cacattccca atgtacaggc
    12481 tgtatcaata cacacgccag tataaacatt tccgctgtcg gtaaggattg ctgcggcaac
    12541 cccaccagca tcgataaaag gtgaaattgt gcgtggattt aattttcccc tagcattatt
    12601 ataaagttcc ttccatttca tcaggttgat cctcctgttg tatccatata aataatcttt
    12661 tatacgcctt ttccgcctaa ggcacacaac acattactcg cttttttcat tctaatattc
    12721 gtgcgtgaaa atgtcaaacg gtttacttcg tttcattact tcatactgct gttcataacc
    12781 ctgtttgttc acttgggcac cattcgagca ccaatttacc cgtttcaggg ttgtagcgaa
    12841 cacagcagaa atgctcattt catgggcttt tctggctaga aaaaaaattt tatcgctgcc
    12901 agatatttac tattttttat taagttgctg gagttccacc accatcagga ggagtagtag
    12961 agccaccacc attatttcca ccattatcag gaggagtagt agagccacca ccattgtttc
    13021 cgccattatc aggaggagta gtagagccac caccattgtt tccgccatta tcaggaggag
    13081 tagtagagcc accaccattg tttccgccat tatcaggagg agtagaaccg ccaccattat
    13141 cacctgtgtt attattgtta ttattatttt cttctggttg gttattgtct ggtttttttg
    13201 tatcttcaga tgatggttga gaacttactt tagtaggagc agtaccttca ataaatgctg
    13261 ctttagagcc agaagattgc tttcctccat cctctaaatt aatttttgta aaatatacat
    13321 ttttaggaac ttcaaattca gtaaccgtta aattagcatg tattttttcc ataatttttt
    13381 cccatagttt tgcagtacta gtacttccat gtggtacttc tctacgtttt atagtgttac
    13441 cagaatcatc ttttctacca gcatcatctc caatataagt tgcaccaaca taataaggag
    13501 tgtaaccgac aaaccaagca tcatatgcac tatttgtagt acctgtttta cctgctacag
    13561 gcattccctt tggaagtgta gcaacaccac cagtaccttc agtaattacg ctttctaaca
    13621 tatctgtaac aacataagct acttcagggt ctacaacttt atgttcttca ggagcatttt
    13681 taactaataa ttgaccatca aaggtagtta tagtagtaaa aatagaaggt tctacataaa
    13741 caccttgatt tgccaatgta ccataagcag cagccatttg aagtggagta ataccattcg
    13801 ccataccacc tagtgtcaat gatgggaatt ttctatcact tgcttcagat gaacctgagt
    13861 gattattctt taaagttgtt ataccaaagt tctttaagta atccatcatt atatctacac
    13921 attcatcata agagtctcca agcatttcag tagttttgac agctatcgtg tttgaagatt
    13981 tagcaagtgc tttacgtaaa ctcatagaac catgaccagc agtagtagtt cttggattcc
    14041 atttattatt ttcttcaaac ttatatcctc ctcgagaatc acttagagca gttgattgtg
    14101 ttatttttag ggtatcaata gcaggtgtat atattgctaa aggttttata gaagaacctg
    14161 gctgatgagg gttagtagca cgattcaaag tttttctacc acttatattt cttcctccag
    14221 ctagtgctct tatttcacca tttttataat ctaagattac cattgcagct tgtggttggc
    14281 taactccatc agggccgacc atatgtcctg ggaaattgct atttctatca aattcttctt
    14341 ctaaagcatc ttgcatttta gggtctatag ttgtgtttac aattagacca ccattgtaga
    14401 ataagttttg agcttcatca tttgtatgtc cttgttccat taaagcattt ataacttcac
    14461 tttcgataag gtcttctaca gatgaagaaa ctttatctga tgattgaggc aattttattt
    14521 ctattttagc tttgattgct tcatcatatt cactttgttt tatatatttc aattctaaca
    14581 ttttctttaa aacaatttct tgtctttttt tagcttcagg gttacttaca gcctttctaa
    14641 caactaatgt acctgctttt aattgtctat aagtatcatt agatataagc tcccaagatt
    14701 ttattttttc aatcatatca aagtccactt gagtagggtc atctaaatca tcatcagttg
    14761 tgttaacaaa aaataataat ttattttcta aatcctcctt agtttcattt ccctctaact
    14821 tagaagtttt ataggctgaa aatcttgatg gatttttagt tgaaccagca agaagtgctg
    14881 actctgctag agtgaggtca gctgcagact tatcaaaata accacgtgca ccagcctcag
    14941 cacctgctag acctctacca acgaagaagt tatttaaata agtttctagt attttatctt
    15001 tacttactgt tttactcatc tcatgtgcat agtaaatatc ctttatttta cgaggtatag
    15061 tttgctgttc agttgttaac agcattttgg atacctgcat aggaatagta ctaccacctt
    15121 gcttagtacc tgtaagggtc tttaatactg acctaaataa acctttaata tcgacaccct
    15181 tatgctcata aaaacgctca tcttctattg caattacagc atgttgaaga taaggggaaa
    15241 tcttatctaa tggaacgggt gtctttttat ttacacttgg agctgtagac aatgtttttc
    15301 cattagcgta tttaatagta gttgtttggt tcgtctgttt atctaaaaca gcttctgtaa
    15361 ctggagttac atctcttagt gacgcaaata ctagaccagc agtacctgct gtaccaacaa
    15421 ctagtaaaac taaaaatact atcccaaata ctcttagttt tttaaattta tcgctttttt
    15481 tagatttttt cttgtttttt gttgaacgag tcttgttagc tgaagttcta ttaattggtt
    15541 tatttgtatt accagatgag ctaacttttt ttctcctgat tttatttcca tttttatcat
    15601 tatcgttatt cataagattt taacctcctt acttaatgca atattataac acaaatagga
    15661 gtagttttgt aattaataga aaaaatgaat ttgctataat attctgtaca ttgtatatgt
    15721 ataaacgtaa tatattaaaa agtctatatt tttattttat ttatttttga caaatattat
    15781 aaaaattatg ccaaagctac atataaataa ataggagggg ataattttga atagaatagt
    15841 agatagaaaa acaaaaaatg aatttagaaa aatcatagct atttttttag taattctttt
    15901 cttgtctgta tttataggca gttttatata tatagataaa actttaagac cgacaataac
    15961 agttttagca gagactaaag ctcttgaact agcaaatagg tcgataaata aagctgttgc
    16021 tgagatggta gaaggaaaaa taaattatga agatttaatg gatatacaac ttgataataa
    16081 tggtaaaata actatgatac aagcaaacac tataatgatg aatgaaattg catctgcaat
    16141 agctttagaa atacaagatg aattaaaaaa ggataagaca gcaagttctt acatacctat
    16201 aggaacagca ttaggaagtc ctatattagc taaatacggt cctaaacttg aagtgtctat
    16261 tgaacctata ggaacagtat cagtaaactt taaaacggaa tttgagtctt caggtataaa
    16321 tcaaactaga catagaatat atttagaagc tcaaacacaa gttaaagtag taataccact
    16381 tataacttca acaaaacaaa ttaaagctca aatacctatt tgtgaaacta taatagtagg
    16441 tgatgttcca gaaagttatg taaatattcc agaaaaaaat ctaggaaatg ttttacctaa
    16501 cactggaaag aataccaaca agtaa
//
LOCUS       DACTGW010000071        16258 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.071, whole genome shotgun sequence.
ACCESSION   DACTGW010000071 DACTGW010000000
VERSION     DACTGW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.071"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(480..2084)
                     /locus_tag="I9573_001806"
     CDS             complement(480..2084)
                     /locus_tag="I9573_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4796429.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGAANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVLDNNREFIKKYINR
                     FKVGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            2470..3396
                     /locus_tag="I9573_001807"
     CDS             2470..3396
                     /locus_tag="I9573_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAT4796430.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTVLYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3516..4514)
                     /locus_tag="I9573_001808"
     CDS             complement(3516..4514)
                     /locus_tag="I9573_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HAT4796431.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMTGKHVGRTVVDLEKI"
     gene            4618..5055
                     /locus_tag="I9573_001809"
     CDS             4618..5055
                     /locus_tag="I9573_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT4796432.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            5141..5728
                     /locus_tag="I9573_001810"
     CDS             5141..5728
                     /locus_tag="I9573_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HAT4796433.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(6028..6648)
                     /locus_tag="I9573_001811"
     CDS             complement(6028..6648)
                     /locus_tag="I9573_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HAT4796434.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(6656..7423)
                     /locus_tag="I9573_001812"
     CDS             complement(6656..7423)
                     /locus_tag="I9573_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HAT4796435.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(7464..8285)
                     /locus_tag="I9573_001813"
     CDS             complement(7464..8285)
                     /locus_tag="I9573_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796436.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(8642..10552)
                     /gene="phnW"
                     /locus_tag="I9573_001814"
     CDS             complement(8642..10552)
                     /gene="phnW"
                     /locus_tag="I9573_001814"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAT4796437.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(10831..11616)
                     /locus_tag="I9573_001815"
     CDS             complement(10831..11616)
                     /locus_tag="I9573_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAT4796438.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(11833..12063)
                     /gene="dltC"
                     /locus_tag="I9573_001816"
     CDS             complement(11833..12063)
                     /gene="dltC"
                     /locus_tag="I9573_001816"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAT4796439.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(12082..13230)
                     /gene="dltB"
                     /locus_tag="I9573_001817"
     CDS             complement(12082..13230)
                     /gene="dltB"
                     /locus_tag="I9573_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAT4796440.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKHISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(13230..14744)
                     /locus_tag="I9573_001818"
     CDS             complement(13230..14744)
                     /locus_tag="I9573_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HAT4796441.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVIFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(14865..16055)
                     /gene="dltD"
                     /locus_tag="I9573_001819"
     CDS             complement(14865..16055)
                     /gene="dltD"
                     /locus_tag="I9573_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAT4796442.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGINPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
BASE COUNT         5345 a         2769 c         1713 g         6431 t
ORIGIN      
        1 atttggatag aattattttt taaattagaa ttattttaat gaatgcttca agtaataggt
       61 atgaaattga atcttttaga actgcttata aaaaatactc aaagaaaaat agtaattttc
      121 tcagattcac aatatatttt atatgaaatg ttataaggta atctatttaa taaatttaaa
      181 tttgctatta tagatttagt tccaaagtct tactgcaaag taatatacat agttttttaa
      241 taaactaata agtgcaatta ataactactt aattgtttaa tatagtacat agataacttt
      301 aataaatcga cagttattta aaaaggtaga atcaataaaa ttatacttat aaatcttcaa
      361 tacaataatt aaaatttaat aaaaaatata attgttgatt taactgtaat cttatttaat
      421 tctacctaac ttattatata aaactatttt ttatagatta taaaatatct aactttcatc
      481 tacttcaaag aatacaatac ttctccttct cttatagtct gcaatacttt aatatctttt
      541 attttcatag ggtcgattgt aagtgggttt tcatctaata ttatcaaatt agctaattta
      601 ccttctttaa ttgaaccttt tttatcttct tcaaaatatt gatatgctgc atttatagta
      661 acacccttta aagcatctaa tgggcttatc ttttcattct ctcctatttg tatacctttt
      721 tttgtaattc tatttacagc acaccatact gtttccagca tattaggtgc tattactggt
      781 gtatcctgat gaagagtgta aattaatccc ttttctatag ttgttttaag tgggctaatc
      841 ttaaacgctc tatcttctcc aaggtttttg atatgaatat caccccaata atatgtgtga
      901 gctacaaagt atgaaggtat catatttatt actttcatat catcaatctg gtctgctctt
      961 actgtttgag catgtatcat aactggtctt atattatttt cactactttg ctcttttaag
     1021 tttaaaactt tctcaaacgc atctatcaac tggtctgctg ctgcatctcc attacaatga
     1081 gttaagagtt gcatcttttc ttttagtgaa atatctataa acttttctac ttcttcatct
     1141 ttgtatatag gatatccaca ataaccatca tctgaatttt catatggtct actcaaccat
     1201 gctgtcttac cttgtggaga accatctaag aaaatcttat aacctcctac tttaaatcta
     1261 tttatatatt tttttataaa ctccctatta ttatctaata cagacttact ttttttcaaa
     1321 tttacatatc ctacaacatc catttttaat ttattttgat ttgccattgc ttttaaaatg
     1381 ttaaattgat tttcttccat taaaccttct tgagctgttg taataccata gcttagatat
     1441 atgttctgtg ctttttcaat tgaattaaaa atctcatttg atgatggttg ttttatttta
     1501 gatgctacat taaaaaatgc attttcttct aaatatccat ttggttcttc actgccttct
     1561 actctaccta tatgtccacc ttctgggtca cgtgtttcat tggtaacacc aagctgtttt
     1621 aaccccaatg tatttgcagc tcccatatga ccagaagcat gtgaaattag tattggattt
     1681 tctgaagatg cactatcgag tacactctta tctggatgct tattttcttc taaaaaatta
     1741 ttatcatatg aaaatccaat tatccagtct ccttttttta tattattcga ctctttgaaa
     1801 tcctgtattt tttttactat gtctttaaaa ctggttgcat cttctaaagg aactagcttt
     1861 agagtagtag caaaagctat caagtgacta tgtgaatcta taaaagatgg catcaatgtc
     1921 ttaccttgta aatctataat ttctgtatct ttatttttca aagcaaatac ttcttctttt
     1981 gttcctatct tttttattat tttatctttt ataagaatag catctccaca tattgaatct
     2041 tccattgtga tgatatttcc attgtaataa attttttctt tcataaaatt ctccttacgt
     2101 tgtctacttt aataaatttt tttcattatt ttctgcttaa ttatattatc ttctttataa
     2161 actttttctt tagagtttaa tcatataaaa gttttatttt taattattgt ttgccaagaa
     2221 gtaaatacta ttcattgtaa ataagacaaa ttaataggct ttatactaat tgaaaaaata
     2281 ttataagttg ttaaatttaa atatctaaac tcaattatat ctgtacaatt gtaatttttt
     2341 aaatcatgat gtattatttt aaatatacag ttacattatt attcacaata taagtatctt
     2401 agttctcaaa taacatctca tgtaatctat tcaatgcaaa aaatttaaat attttttagg
     2461 aggtttttta tgagcaagga aaataaaaat gtaagaattg ctgttgtcca ggcgtcacca
     2521 gtcataatgg atttagaaaa aactgttgaa aaagcattag gattaattaa agaagctggt
     2581 agaaaagggg caaatatcgt agttttccct gaagcattca tacctgcata tccaagaggt
     2641 ctttcatttg gatttgttgt tggaagtaga acaatggaag gtagagaaga ttggaaaaga
     2701 tactatgata attctgtacc agttcctagt gctacaacag atttattagg aaaagcagca
     2761 caagaagcag gagtatatct atcaatgggt ataacagaaa gagatggtaa tgatataaat
     2821 tgtacccttt attgcacaaa cttatttttc tctccagaag gtaagctaat tggtaaacac
     2881 agaaaactta aacctacagg aacagaaaga tgtatttggg gagaaggaga tggaagtact
     2941 cttacagtag ttgatactcc atatggaaaa atgggttcgt tgatttgttg ggaaaattat
     3001 atgccattag ctagaacagt tctttatgct aagggtgtaa aactttacat agcccctact
     3061 gcagattcaa gagaagagtg gcaagcaact atgaagcata ttgctttaga aggaagatgt
     3121 tttgtaattg gatgcaatca atatgttgaa aaaaatatgt atccaactga ccttaactac
     3181 tataaggagc tagatgcaga accagaaatt atgtgtccag gaggaagttg tatagttgac
     3241 ccttttggaa aatatgtagc aggtcctatt ttcaataaag aagaaatgct aatagctgat
     3301 ttagacctag aaaaaatagt acttagtaga ctagattttg attcagaagg tcactattca
     3361 agaccagatg tatttgaatt aattgttcat gaataatctt tttacacaaa catctacata
     3421 aattttttta cataaaaaaa tgagatactc ataagtaaat ttcaagtatc tcatttttgc
     3481 ttaaaaccta taatcctaat tataaattta ttcgcctata ttttttctaa atcaactact
     3541 gttcttccaa catgttttcc tgtcattatc gaatctatac tatcatttaa ctcttccaat
     3601 gatactacat ttaccatatc atctaaatca tctaatttcc attcatttga taataaatcc
     3661 catattttta atcttaagtt cattgggcaa tccacagaat ctataccata taaagtaatt
     3721 ccctttaaga taaatggata tactgaactt gtaaaatcaa ctcctcctgc attaccacaa
     3781 gttgttactg ctccacaata attagttgtc tttattgctg ttgctagagt atttcctcct
     3841 actgtatcta ttactccagc ccatcttggt ctaagcagag gtcttcctga gttatcatcc
     3901 acttctgacc tatgtataac atctttaaca cctaagttta gtaatacttc tttttgttct
     3961 tctaattttc ctgttgaaac tacaactgaa taacctaatt taatcaatat tttagttgca
     4021 attacagcaa ctcctccagt accaccagtg actaatacat ctcccatatt cttgtctaca
     4081 gaatctataa atttatacac agataaagct gctgtaaacc ctgctgtacc ataatccatt
     4141 gcttctttta gtgaaagatt tttaggaagt ttaactaccc aactagaagg aactcttatg
     4201 tattctgaaa aaccaccaga agtattcatt cccaagtcat aacctgttac taaaacttct
     4261 tctccaattt taaaatcatc acaactacta gattctacta taccagatgc atcaattcct
     4321 ggtgtatgag gatactttct cgtaacaccc ttattaccat ttgcagaaag tgcatccttg
     4381 taatttagag aagaatattt tacttttatt agaacttcgc cttctggcaa tgtatctact
     4441 tctctttcaa ctatatttct tctaaaattt ccttcttctt ctgtaatata tagtgattta
     4501 aattttttat tcataaattt cctcccaata catttagttt tatgttaaac taattatagt
     4561 ttataaatag ttttatgtca aactaattta aatagattaa agattggaga ataatttatg
     4621 gaagataaat atattgtaca tttcataagt aaaacaaagg ctagtatgat taaattcatt
     4681 gaaaataaac tcagtaaaaa tggtttaggt gagttaatac ctactcatgg aaatatttta
     4741 actgcacttt atgaaaataa tggaatactt acaatgaaag aaattgcagc taaaatagga
     4801 aaagataaat ccactgttac agtgttggtt aataaactta taaacctagg atatttagaa
     4861 agacaaaaat gtacaaatga caaacgtatc acgtatataa agcttacaaa aaaggcactg
     4921 cttatagaag atacttttaa tagcatctca acacaagtta aagagacagc ataccataat
     4981 attacagaaa atgaaaaaca agaatttctt agaattttaa aaaaaattaa tgataatttt
     5041 aaaaatgctg actaaattga gcataattat atctcctatt ggaataataa ataaaaagaa
     5101 tcaaatattg taaaaattta ttatgataga gaggtagaat atgaatttaa aagatagtaa
     5161 aactaaagaa aatttattaa gagcttttgc aggtgagagt caagctagaa atagatacaa
     5221 tatttctgca tctgtagcaa aaaaagaagg attatatata atagagtctt tatttaatta
     5281 cactgctgac caagaaaaat cacatgcaaa acaattcttt tctaaattaa aagaatttgc
     5341 tggtgaaaat ataagtatat caggagcata ccctgtagat ttatatgaca agacagcaca
     5401 acaccttaaa gctgcacaac ataatgagct tcaagagtgg gatgatgtat ataaaaactt
     5461 tggaaaaaca gcacgtgaag aaggatttga atctatagct aagttatttg aaaatatagc
     5521 tgaaatagaa aaagttcatg gtgatagatt tggtagatat gctactgatt tagaagaagg
     5581 tactttattc agaagaaatg aagatataca atggatatgt acacattgtg gccatataca
     5641 tataggtaaa gaagctccaa aagcttgtcc tgtatgtctt catccacaag gatatttctt
     5701 agattttaaa aactcattat ttgaataaac taaggcaata attctattta aacaatttac
     5761 tatatttata tcaagtattt ttataaatac tgtattatct taataacaat aaaatgaacc
     5821 attgatatta ctataagtag aaaatgtagt ttaaatacaa tttatagtaa ttcatcatgg
     5881 ttcattttat tatttactaa tccctataac ctacttaaat ctatctttaa cttttacata
     5941 ctcacataaa atcttatctt taaactaaat atttaaataa agtgtatttt aagtagatta
     6001 ttataataca ttatataaga taatacacta tgataacttt atacttatat catttatttc
     6061 ttttgtcaag tcaatctcaa taatcttaac tacatctttt tcaaacttta cttttttata
     6121 atccagacat cctactacga atggaactcc aaactttcta aacaaataaa tctttccact
     6181 ttcattgtat attgatgtaa aattttcttc tattatattc actatcaatt ttaactcttc
     6241 aaaagttatt atagatttaa ctaaaaactt ctctattatg ctcaatataa gcagttcttt
     6301 tttacctatg ctttgctctt ttttatataa attttgaaat atttctttgg tataattaca
     6361 tataacttct ttactgagaa taaaatcaat gtcaaattcc ttattaatat cctttttcat
     6421 aaacatattt gcaatatcat caagagaagt ctcttctttc atagacaata ttaactctat
     6481 tctttctagt attttatctc ttggaaagaa agtctcttga cctgtaaaag ctgatttttt
     6541 tataaaccat tcctctggta ttatgttttt tcttttccat ctatataatt gaccataaga
     6601 tatattagtt ttgctcaata aatctttctt agatattaat tcttccataa cttttctagc
     6661 ccacctttat actgtttttt acagtagaat tatctgaaac tgataaatta tcactgtatt
     6721 caatatttcc aacgtcacaa ttatctacta gagatatgct tttgcctcgt atatctttta
     6781 ttgatgcatc ttctaattca atagtatctc cctctatcaa attacattta aactttcctg
     6841 aaaaaatatt aatgatttta attaatgaaa aagcatcttt gcttcttcct atttttatat
     6901 tagttgcccc tatctcttta caataagaac ctcctcctga ataaacatat atattttctc
     6961 catttaaaag accatcacat tttattactc catatacctt tatatcttca cattctatat
     7021 ttgatttatt tctcatttcg ccatagattt ttatatcttg tgcatacata ttttcattta
     7081 cacttaattg tcctcttatt gtaacattat tacatttaag tttgccatcc actttctgtt
     7141 gagcagttat aactaagtca tctatttctg catctccctc tatattaagt tgaccattca
     7201 ctcttacacg ctctgcttta agattgcctc ttataatagt ttttccgtta acaacaaaat
     7261 tttttatatt gatgtcacct tcaaattttg catctccaag gacttttact tcgttacttt
     7321 taacatttcc ttttgattta gaacttccca cgatttttat attttcatac tcaccactac
     7381 ttataaagcc ttctccaagt atttttatag accctttttc catattatta cctcatttta
     7441 aattttattt ttaaacctat ttattactta tccttcttta attttatttt ttcacttata
     7501 ttctttattt caccaactat tgaatcttca tcaatctcta tactttcact gtactccaaa
     7561 ctttctacat aacatccctt gcctatttta atattttttc ctcttacaac tttgacttca
     7621 ctattttcta agcttatttc atctgcttct ataatatttg caaacaattt actttctgta
     7681 tactttctaa ttgataatag acctaaactt attaaatttg aagacacttt ttcaaagttg
     7741 atatgctttt tttctagcat acgaaatttt ttgcaataat ctccatgccc cttttttata
     7801 tttatactac ttgctccaac ttcattaaaa ttagaagttc catttaaact aatattaact
     7861 tcttcacaat tcaaaaatcc attacactcc aagattccac ttaaattgat attttcacaa
     7921 tctatattct catttgcttt taaatatcct tctccatgaa taatatccga ttttatactt
     7981 ccttctacat taataattcc actagtatca atttcaacag aattaatatt tcctgtacat
     8041 ctaagaagtc ccgatatctc aacttttgat gccagtatgt ttccttctac tttacttaaa
     8101 ccagatgtaa aaaattcatt acattctgtt gtcccaaaaa cttttgctga accagaagaa
     8161 tcaaatttac tacattttac acttccttct attttgcctg ttcctgatat attaacatta
     8221 tcaaaaattc cacctttaca ttttccaaca ccatcaaatc ttagtgaact taattcttca
     8281 ttcataatga tatcctttct ttaccgtatt ttatctttaa acctaatata acaaaacatt
     8341 gttacgttgt caagtctgat aataatactt aataaagcaa ctatcattta ttttctataa
     8401 ataggcataa aaataatagg gaaatgcttt atttaaacta gaaatctaat ttcttagatt
     8461 gtggtttaaa taaaatatat ccctattact caatctttat atattcattg tataaacttc
     8521 taaaatataa gtctttaaat tctactgtca atacttttta tacactgttt gtgctatcat
     8581 ttcacaccta aaggagtagg ctctccgcac tattctgtaa cctaaaagac actatcttct
     8641 tctatctatt gataaatttt tctattacat cagctagtct ctccatatca gttggataaa
     8701 cttcacctat gctacctatt ctaaatgtat ctatatcagt caatttacct ggatagataa
     8761 caaatccatt atcttttaaa taagtataaa attccataaa ttcaaacttt gcatttttag
     8821 gatataagaa agttgttatt acaggtgatt gagcattatc attcaccaaa gtatcaaatc
     8881 ctaaagattt taatcttgaa gctattgtaa actgattttc tttatatcta gcatatcttt
     8941 tttcaacaga accttcttct tccaattcta ataaagcttg ataaaaagct cttacaacat
     9001 gtgtaggtga agtaaatctc catttaccat tatttttctc catagtctcc cattgcgcat
     9061 acacatcaag agataatgat ttagctatac ctttacattt agataatttc tctttattag
     9121 ctattataaa accaaatcca ggtactcctt gtatacattt gttagaacta ctaactaaaa
     9181 aatctatatt aaaatcttca acatcaatct caattccccc aaaactactc atagcatcaa
     9241 ctatataaat tttgttatat tcttttgcaa gtttccctac ctcttgtata ggatttaatc
     9301 tacctgtagt agtttcacag tgaaccattg atatatgtgt tatatccttg ttttctttta
     9361 ataaattttc tactacattt aaatccactg cttctatatc tttaaatgtg cagtcaacaa
     9421 attctatgtt aagataatta catatatctt tcattctctt tccatatgct ccatttgcaa
     9481 ttacaagaag tttaccatct tttgaaatag tacttcctat tatagcttct actgaaaaag
     9541 tcccacttcc ttgcattaat acacttgtgt atttatctgt attttgggtt gctagagata
     9601 ctaatcttct tctaacatct tgtactaggt tattatattc aacatcccaa gtacaccaat
     9661 cttttaacat agatgctctt actgatttag tagtagatag tggtcctggt gtaagtaata
     9721 tgtaatcatt ttctggtaca aaatctgatt taatagtttc attctctatt tttattagta
     9781 tatcttctaa ctcagccata gtttctatta caaaatgtgc acctgcctct ttaaatttct
     9841 ttgaaactat actcatctta gcttttaatt cttccttatc catgttctct acttcttctt
     9901 gtgtaagccc taactcactt gaacctttta ttacagcaac actccacatt cctgcattta
     9961 cgccttcttt tacatcactt attgtatctc ctacttttac catagaactc ataggagaca
    10021 cccctaaagc ttcagcattt ttatagcaca tccaaggata cggtcttcct tgagatactt
    10081 ctgatggtgt tacaagaaaa tcgggtgagt atcctttctt tgctgcattt ggttcaacta
    10141 tgttcatcat ctctcttgta taacccgttg tagaacctat ttttaatcca ttttttctca
    10201 acttttcaat agtttcaaca acatgaggaa ttggagtcgt atactcttct aatgtctcaa
    10261 ataacattgg ctcaaattca gcataaagtt catttacatc atcttcagtt ggcactttac
    10321 caaacttatc tgaccaaaga ttttttattc tatccatttc acacatttct cttatatggt
    10381 ctatttttaa ttttcccata ggttttcttg cttcttccat tgttacatct atacctcttc
    10441 ttttaaatat ctctataaat acattaagag gtgcaaaaca tccataatct actgttgttc
    10501 cagcccaatc gaatactact gcttttattt tttctccata tatctttttc atatttatag
    10561 cctccataga ttcattttag aatttttgtg tttttaattg tttttacttt taaagtataa
    10621 ctttcatatg ttaattatac atcatattta ggttaaatat atattaaatt tctgttttta
    10681 gttgtattta attgttttta ttttatatca tatttccata aaatcctaag ttacaatcta
    10741 aaatttactt tctaatctta atctatgaac tgactatatt ttatgaattt agttgtatta
    10801 agcatagttg tattatataa aactaagggt ttattttgct tctatccatg ttatatttat
    10861 ctcatttaac ttatccttcc attcattcgg aggttctaca tcacaaataa caaaatctaa
    10921 ttcctctaaa tctgctatct tatacatact agcaattcct attttagaac tatcacataa
    10981 tacaaaactt tcctttgaat tttttatata ttcttttgac aacaatgcct tattttggtc
    11041 tatactacta attccaaaac tatcaagtat accttcacta gatataaaag ctttatctac
    11101 gtataagttt ttcatagtgt ctattgacat tggacatgct gtcctttggt gttttaaatt
    11161 tacttttcca ccaataaata taacttctcc atcaaacaaa ccttgattct cataagaaat
    11221 caattttgat agaactggat aagagtttgt aataattttt aaattgttta cagatattat
    11281 ataatctaca atctgcaaat tggtagtacc ttcatctatg ataattgact cattgtcatt
    11341 tacaaactta gctgcgtatt tagcaatact ttgtttttta tctaggttag tattgtttct
    11401 ctttatatgt tctggttcaa tcactccata atttattttt atagcaccac catatacctt
    11461 ttttaattta ttttcatttt ctaattcttc caaatatctt cttattgttt ctgatgatac
    11521 ttctaatctt tttactaatt ctttagtatt taccttccct tttgaatcta ataatttaag
    11581 tatttctttt tttctatcta tcgaatttaa agacattcat tttcctccta aatataatat
    11641 atttttctcc tataatatta caatgatacc taagatttat gaaatcatat ttatgtatta
    11701 aattttttac ttaattaaag ttattttcaa aagactttgt attatcatta aaaaagtcca
    11761 tcaaatatta gtatcagatg gactttgtaa aattttattt tataaattaa tatttaaagt
    11821 ctaactacta atttattttc ttgtctccaa gaattttact aaattattta cagtagacat
    11881 atcttttctc tctaaatcag ttggttgaag ctctatccct aatctatttt caatttcaag
    11941 taaaacttca attatagcta atgagtctaa aagttccgtc tcaaataaat taaggtctaa
    12001 atcttctctt atctcatcag tccctaatac atcttcaaat atttctataa ctgtttcttg
    12061 cataaatttt cctccttaaa attaagctaa ataacctgag aatatcagca atccaaaaca
    12121 cacaatatgg aatgtaatga aaatctgcac tctctcgaac catttatctt ttttatgttt
    12181 tttataaaat ttagattttc gctgatatat atcagttaac acaagtgcca atccttggta
    12241 tacaccataa gcaacataat accaagttag accatgccaa aatcccattg ttatcatggt
    12301 tatcatctga gccacatgag cagcagtagt ccttttctta aatcttttct ttctcattga
    12361 tgacataaca aatctagaaa aaatataatc tccaaaccat cttgaaagac ttatatgcca
    12421 tcttgtccag aactctttca tatctttact gataaatggc ttgtcaaagt ttataggtac
    12481 ttgtattcca aatatgtacc ctgtacctat agcaaataaa ctatatcctg caaaatcaaa
    12541 gaacaggtat aaactatatg cgtacatata acttaaaata tttataaaac tcatatcttt
    12601 aggtattctt gatacccaat atgtatttat caaaaatgct ataacaaact tataacctac
    12661 tcccatgaca atatttttta ttcctggcag taagtattct tctatatact cttttctaga
    12721 gatgtgtttt tccaagtcct gttcaaatct tcttgaccta tcaataggtc cagaacttaa
    12781 agttggaaaa aataacataa aataaagcat ctttgaaatt ttaacttctt ttatagctcc
    12841 atcataaatt tcaataacca tttgtatagt tctgaaattt aagtatgata ttccaataaa
    12901 tccaattatt ccaaaagaag taactggaga tattttattt attattattg ggagcattga
    12961 tgcaaacaag aatccccaat atatgtattt atttttagtt tttcttctta catattcata
    13021 tcctttaact attattactt caagaataat aaatattacc aaatacttta gctggacatc
    13081 aattcctact attaagaata tcataaacaa acttgccaac ataccataat actttatatt
    13141 cttgcccatt agacctaaaa ttactgctgg tattgatgtt aataataata tataaagata
    13201 aaaataatct ccatactgtg aaaaaatcat taaatttcct ccataagttt ctttctgtct
    13261 attttaccat tgatatttgt tggaaactca gagattattg aaatatttct tggaatcata
    13321 tatgaaggta tatactttcc aagttccttt tttattatca tcccattttt aatattactt
    13381 aaatcattct tttcatttaa ctcaacaata ccttttaagt aagcaatttt ttcgtcctta
    13441 tatactggta aaactactgc attttttaca ttatgtactt ttctcaaatt attttctata
    13501 tcttctattt caattctaaa tccatttaat ttaatctgaa aatcttttct tccacaataa
    13561 tatatatttc catctaaaag atatcccata tctccagttt tataagctct ccactttaca
    13621 ccatctattt catcatagaa aaatacttca tcagtctttt ctttattatt gaagtaacct
    13681 ttagaaactg atggtccaat tattataatt tcaccctttt cattttcttt aagttcattt
    13741 ccatcttcat caagtatttt tattttacaa tttgacatag ggtatccaac tggaagactt
    13801 ttttcatcat ctatagcttt ttgagtcata tcatttacac ttacaccaac agtagcctcg
    13861 gttggtccat atccattgat aattcttgtg tttggaaatc tactcataag ttctttagta
    13921 agattttttg aaagtgcttc tcctatgaat atcattgact caaggttagg aagcatttta
    13981 ctattaaact ctttttcagt tatacatact cctgcaaaag aaggagttga cacccataca
    14041 gctatatctg agatactaaa ttgtctaaat agttctttat agtctgctaa tacatctttt
    14101 gatattgaaa ataatgtagc tccatggata agaccagggt atattgttgt aactgaaagg
    14161 tcaaaagaat atgctggttg gttcattatt actttttcac ttccatctat gtttagatat
    14221 gggcttatcc aatctgaaaa gctatctaaa ttgtttgaac ttatttgaac cccttttggc
    14281 tttcctgtac ttcctgaagt aaatagtata tatgcatttt catcatcttt tacccagttt
    14341 tctttatcta atgattttcc ctgatattca tttattatat catttaattt atccatatca
    14401 attacattga tatctccaaa atttctttca tcactaaaat taaatattac ttttggtttt
    14461 atctctttag tgacttcaaa aactctatca attggaaaac taatatcaag aggcacatac
    14521 gctctacctg attttaaagc tcctatcata catgccatta tcatgttttc tttatttcca
    14581 taaattacta taggagtatc ctcttctttg tatacatcct ttagaaatac tgatatagca
    14641 tctgaatatt catttaaatc tttataagaa agtttatctc cattacacat tagtgctgtt
    14701 ctatctgtat ttgaatattt ttttattcct tcaataattt tcatataaga atgctccttt
    14761 aaaatccgtt ataaataaat ggcaattctt taaatggagt aaatgttaga actcctatcg
    14821 ccaatattat aaccatagtt aaacaaatac tagttaacaa atctttattg ttctttaaat
    14881 attttaaata atctctcaca gacatctacc catcctttcg taccaagatg cattacatcc
    14941 cttaaataat acttatctga ccctttatct ttaaggttta gaacttcaaa gcctttgctt
    15001 tctgctattt tttgtgcttt atcgtagtac tgatttcttt ctttttcaga aataccagtc
    15061 aaattataga atttatccat acttgggatt aatacaacaa ctggattaat acctaagtca
    15121 gtacatacat ttagcatcag tttataattt tcaaactcct tagatgttaa taaatttaca
    15181 tctttgtctc ttcctttgta ttgctctata ccatctttaa aatgctcttt ataatagtac
    15241 ttatcaatat ttagtggatt tttaccaact ctttttttag catcttcaat ggctttcttt
    15301 ctctcatatg accaatttat tggtcttttt ctttttgttg ctctcttttt atctagctta
    15361 attaatcgtt tataaagaat acccttttct ttaagagcta tacagtactt tcttccttga
    15421 aaataaggct ctaaaagcac cttttcagct ttttcaggaa atgtcttagg ttcatatagc
    15481 ttagcatata atgcttctgc tttatactca tcacttcccc ataatagctt acttgatttt
    15541 tttgcatatt caatcttatt ttgtttagat atttttggat tatctaaaaa tcgataaaat
    15601 tgtattggtg aaaatctaga ctgataatgg tgacttgtga ctccgtcttt ttccataaac
    15661 cattgcatag aaattaacaa gacaactttc ttattatcaa tattaggatt catacttcct
    15721 aatattgcag catcttgtaa tgtctgagta taggctctcc caattgcaaa caccttattt
    15781 ttactcctat ttgtattaaa atagtacttt ggatgttgtt ttgttgaatt actaagttct
    15841 gatgacccca aaatcataat atccttttct cttaaaaaat gattatttgc tgtaacccct
    15901 ttatctttta tatcacttaa agtatcatcc ataattggaa gtagattttt ctccctcaaa
    15961 agttcatcag ttttagaatc aagaaatttg tctaacccaa ataaaaatac tacgcctatt
    16021 ataaaaggtg taataaagta tatcaatttt ctcatatttt ctctcctcta aaaatattca
    16081 aatttatgtt attattaatc atattagttt tgatatgtat attcaactac aaaattgtaa
    16141 taaaatcgtc aaaacttttt ttgatactgt tacaaaattg taaaaaaaaa gaaaaaaaca
    16201 ccaaaaataa atttcttttt agcacttttt catacttata atttgacatt ttaacact
//
LOCUS       DACTGW010000072        15789 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.072, whole genome shotgun sequence.
ACCESSION   DACTGW010000072 DACTGW010000000
VERSION     DACTGW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15789
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.072"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            46..588
                     /locus_tag="I9573_001820"
     CDS             46..588
                     /locus_tag="I9573_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT4796443.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            913..2406
                     /locus_tag="I9573_001821"
     CDS             913..2406
                     /locus_tag="I9573_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAT4796444.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDCNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINKGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKVNELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            2643..3407
                     /locus_tag="I9573_001822"
     CDS             2643..3407
                     /locus_tag="I9573_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT4796445.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEE
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(3692..6871)
                     /locus_tag="I9573_001823"
     CDS             complement(3692..6871)
                     /locus_tag="I9573_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAT4796446.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKRDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKETTIENKLTSSQFTKENNHYVFKGTDYDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIYKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIIDLFN"
     gene            7152..9329
                     /locus_tag="I9573_001824"
     CDS             7152..9329
                     /locus_tag="I9573_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAT4796447.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALHSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRHMFKKTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDNEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYRESDINSIIDDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            9343..9765
                     /locus_tag="I9573_001825"
     CDS             9343..9765
                     /locus_tag="I9573_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAT4796448.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGACPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            10172..10975
                     /locus_tag="I9573_001826"
     CDS             10172..10975
                     /locus_tag="I9573_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAT4796449.1"
                     /translation="MKKLKLIIMLALVLALLITGCSSGNNKNKEEQKTIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMVEPPIQFGAC
                     SFAIQKNEKGEKWTKEINEVIEEMHKDGTLTKISEAWLGKDITKEPINE"
     gene            10968..11636
                     /locus_tag="I9573_001827"
     CDS             10968..11636
                     /locus_tag="I9573_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAT4796450.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMTEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     GKAY"
     gene            11872..12534
                     /locus_tag="I9573_001828"
     CDS             11872..12534
                     /locus_tag="I9573_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAT4796451.1"
                     /translation="MIFNMKYFLELFPIIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMNDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDKGQIEAAYVMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            12594..13391
                     /locus_tag="I9573_001829"
     CDS             12594..13391
                     /locus_tag="I9573_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT4796452.1"
                     /translation="MVKRNIIKCLVAVLSLTFVVAGCSKKEEVKDEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVAASVNKD
                     NLFEEKVYGDGTNGLQDLDLGRVSAWIDAELTVVGDAKKANMKVRVLDEKLSVTNIAY
                     FFKKNDEKSKTIKEDVNKAIEEMLSDGTVKKISEKWFGMDVSADIQK"
     gene            13524..14699
                     /locus_tag="I9573_001830"
     CDS             13524..14699
                     /locus_tag="I9573_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4796453.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEFKTSKLIKEE
                     LNKLKIEYIDVAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKILENIV
                     RNVCRAYGADCKFEKSTGGDAVINDKNMIELGRESACKILGVENVEIIDFPAMTGEDF
                     AIYMKEKPGLFMYIGVGNKEKNINYRLHSNKFNIDEKCLSIASSLFSQLAVDYLCQL"
     gene            14808..15557
                     /locus_tag="I9573_001831"
     CDS             14808..15557
                     /locus_tag="I9573_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAT4796454.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEINQSEVVAILGPSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDIANLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSNKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIVRKEEVFS"
BASE COUNT         6148 a         1665 c         2597 g         5379 t
ORIGIN      
        1 ttactattat ataaaaattg gcttaaaaga aggtgaaaaa agtaaatgaa aatatgcata
       61 acagtaggtc atagtatact aaaaggtggt aaaagtacag gtgtaaatgg tatagttgat
      121 gaatacaatt ataataaaaa attagctcct atgttagctg agatgcttat aagtcaaggt
      181 aatacagtag atgtaataat ttgtccagaa aggcacttta tgtcagaaag agaagaattt
      241 ttctatagag ttccaaaagt aaacagtggt aaatacgatt tattagtaga gctacattta
      301 aataaagctg atggaacaca atgtggtaca gaagtgcttt actatggcac tgaaggatta
      361 gaatatgcta aaagagtatc taatagactt ggagaattat ttgaaaatag aggagctaaa
      421 aaaagagaaa atttatatat attgagaaat acaaatccag ttgcaataca gattgaaagt
      481 tttttctgtg ataatgtgaa tgattgtaac aaagcaaatg aatcaggtta tgattatata
      541 gcacgattga ttacagaagg tatattgaat aaagatataa atatgtgaga ttatggtgat
      601 ttaggtattg attaatacct tttaatatta gatataaata aaaatacaat gtattagtat
      661 aatttaaact ttcaaaatat agattagttt gattattgac aagagatacc tcctaaatat
      721 tgatttatca gtatatttaa ggaggtattt aaatataaaa atttaattga tttgctacta
      781 aactataggt ttatagagta tatattttta tcttcagtct aataaataat ataaaaatag
      841 tagaaccttg tattataata atataatgta gaaaaatata tagataagat aaatataata
      901 aggtggtaat atatgaaata tacatttgaa aaatttaagt taaatgaaaa gatacttaag
      961 tcattgaaga gtttaggata taatatacct tctagagtac aaagagaagt tattccaaaa
     1021 ttattaaaag gtcaaaactt agtagttagg tcaaaaacag gaagtggaaa gacagcaagt
     1081 tttgcaatac ctttatgcga gaatataaat gtagattgca ataatattca agctcttata
     1141 gtggttccaa caagagaatt agccttgcaa gtaaaagatg agatatcaga tatagggaga
     1201 ctaaagaagg tgagatgtag tgctatattt ggcaaacaat ctataaaaga ccaaatagct
     1261 gaattgaaac aaagggttca tattgttgta gctactccag gcagaatact tgaccatata
     1321 aataaaggtt ctattaagtt ggaaaatgta aagtatttag taatagatga ggcagataaa
     1381 atgtttaata aaggttttgt tgaacaaatg gaaaaaatat tattaaattt acctaaggag
     1441 aaaatagtaa gtcttttttc agctacaata gatgaagaga taaaatatat atgtgaaaaa
     1501 tatatgctag attacagtgt tataaatata gaagaaaatg agagcgatac taatcaaaaa
     1561 acaagacaaa tagatgacaa aataatcaag gcaaatggaa gagagaagta catcctttta
     1621 aaagaattaa tatacagcga aaatccaaag agtgtgataa tattttgtaa taccaaagag
     1681 aaagtatcta aattatataa taagatgagt aaagagggtt ttttaataag agagttacat
     1741 gcagatttat cacaagaaag aagaattttt gtaattaagg attttaaaaa ccaaaaattt
     1801 aatatattag ttagtagtga tgtagcgtcc agaggaatac atattgatga tatttcttta
     1861 gtaataaatt atgatgtgcc acaagataaa gaaaactata tacatagaat aggtagaact
     1921 gggagaaaag gaaatagtgg aaaggcaatt actatagtaa ctgaaaagga tgaaaaatat
     1981 atagagaata tagaaacgta tataggatat aaagtaaatg aattaaaaga tatagaaaaa
     2041 agtagaatta cacatggcaa aattttgttt gaagaatatt caaaaggaat cctaaaaaaa
     2101 gtatcaaaga gaaaaaaagt tgataaaaat tcttacaaaa gtaaagatat aaaactaaat
     2161 atagaaagtg aagttgttaa attatattta aatgctggta agaagaagaa aataagagtt
     2221 ttagatatag ttggtgcatt tagtaatata aaaggtataa ctaatgatga tataggtgta
     2281 attgaagttc aagatttatg ttcatatgta gatatattaa attataaggg agatttgata
     2341 ttaaaaaaat ataaggagat acctataaag aaaaaaatgg ttaaagtgaa aagagatata
     2401 cgataattta gttattactg ctctctctga tgatgaaatc atatatttaa gtaagagtat
     2461 caatatttat gaattattct gttatcgaaa tgaagtttca aaaaacaata gaagttgtta
     2521 aggaaaggaa tgttgacttt atctacattc taagctatac tatttttaaa tgtttatgac
     2581 aatagtcatt ttaaatataa gtaataaata tatacctata gggatattaa aggaggtaat
     2641 atatggcata ttttaactat aacgacaaac agtgctacta taatgaaatt ggggatggta
     2701 caccattact ttttttacat ggaaatacag cctcttccaa aatgtttaat gatataatcg
     2761 atttttataa agatgaatat aaagtaatat taattgattt tgtaggtcat ggaaaatctc
     2821 aaatggtcga taaattttct gctgacttat ggtttgatga ggctatgcaa gtgatatgtt
     2881 ttttagaagc aatgaactat aaaaaagtaa acataattgg tagtagtgga ggagctcttg
     2941 ttgcattaaa tgttgcactt gagagacctg atttagtgaa taaggtaatt gcagatagtt
     3001 ttgaaggaga agttccttta gagtcatttg tacaaaatgt taaaatagag cgtgaggcat
     3061 caaagcaaga tgatggtgca aaagcttttt atatttataa tcaaggagaa aactgggaag
     3121 aagttgtaga taatgataca gaagctattt ttgaacatta taaaactata ggaaaatttt
     3181 ttcataagcc tctagaaact atgcaaccag aggtgctttt aacaggaagt agagaagatg
     3241 agtttgtatc tctaatatct agtgattttt ttgaaaatac tttttcatta cttcttgaaa
     3301 agattaaaaa tggtaaaatg tatttgtttg ataaaggtgg tcatcctgca attttatcga
     3361 atggattaga tttttcaaat gtagcaaaga aatttttaga ggaataattt aaccaaaatt
     3421 tacaataatg aaaactgtaa aatggattgc aaaacttaca aaagtgagtg tacatatact
     3481 tcattattat gatgaaatag gattgttaaa acaaaatatt ctaatagtca agttattaca
     3541 tttgtatatg caatatgtat agaaagagat atcattagat tgtaaagttg ggattaaata
     3601 acaatatgaa attttattta ttcagatatt gtagcttaac tatataaatg ttagaataaa
     3661 tttatatagt taagctgtag gattaaataa tttagttaaa taagtctatt atctcttcat
     3721 cagatagact ttttaataca ccttcattag aaagttctcc atttataaat tgatttataa
     3781 gctctttttt actttcttgt aattttatta ttttttcttc tatagtccct ttagcaatca
     3841 attttataac ttgtactgaa tttttttgac caaatctatg agctctatca ctagcttgat
     3901 tttctactga tggattccac catgggtcaa agtgaattac catgtcagca gatgtaagat
     3961 ttaaaccagt tccgcctgct tttaaagata ttaaaaaaac ttttttatcc atactattat
     4021 tgaactcatc tacaagttca agtctttctt tggcattagt tttaccatct atataatggt
     4081 atttaatttt atacttatca agttccttac ttatgttctt aagcacagaa gtaaattgtg
     4141 aaaacaataa tattttgtgg ttttcgttta tactatccct aagtatttct aaacatgttt
     4201 caatttttga acttttttta ttgtaatctt ttactactat acttgggtct aaacaaagtt
     4261 gtcttagttt agttaaataa gaaaatataa ctattttatc tttctttaga tttttatctt
     4321 tcattttatc ttgtatatct tttgaatata cactatatat ctttttctgc tctttattca
     4381 attcaacaaa aaagtttttt tcaatcttat ctggaagttc tttcataact tgttttttac
     4441 ttcttctaag aataaaaggt tttattaatt ttttcaggtt tttaacatta tcctctttat
     4501 ttataaacaa ttcctgaaac tttgctttac tatatagata gccaggcatt ataaaatcaa
     4561 atatagacca taattctagt agattatttt ccataggtgt accagtgagt gcaaatttat
     4621 tttctgcatt tatacttttt acagactcag ttactaaagc tactggattt tttatatttt
     4681 gagcttcatc taatatacaa taatcaaact ttatttcact atatttttct aaatcatttc
     4741 taagagtacc atatgttgta agtattacat caaaattttc taattccata aaacattttt
     4801 ctctatctct tttattccca tgtatcaata aaacttttat atctggagca aacttttcaa
     4861 attcattttt ccaattataa atgagtgatg tcggtgtaac aataagactt tttttattag
     4921 ataatgataa taaaaatgca atagtctgta ttgttttacc aagacccatt tcatcagcta
     4981 atataccacc aaatttataa taatctaaaa ctttaaacca attgaggcca tttatttgat
     5041 aatcacgaag gtttgcattt aaatttgcgg gaatttctaa atttatgcta tcaaaatttc
     5101 taaatttttc acatatactg tctagttctt ttttaccatc tataaacttt aaatttttac
     5161 tacgtattac atcatttata tatagagctt tatttttata aattttatta ttttttgatt
     5221 tatccattat atctagattt tcaactaatt caaaaaaatc ttttgtttct ctttctgata
     5281 aatctataaa gtttccatct ttaagtttat aaaaagtacg tttttcttga aaagctttca
     5341 aaatatcctt gtactctttt ggatttatat cagatatatt aaaattaaat tctaggtaat
     5401 tctggttagt ctgattgatt gaagcttgaa ttgatgaagc gttatatatt tttttcaatt
     5461 taaacttctc agaataatat atatctccaa attcctttag agattctaat tgtaaatcta
     5521 aaaatgtaaa taggtcatag tcagttcctt taaacacata atgattattt tcctttgtaa
     5581 attgtgaaga agtaagttta ttttcaatag tagtttcctt ttgtatgttg gggagcacaa
     5641 attttttatc ctcatcatca tatatatact ttacatcaca acatatgttg ccatcaatca
     5701 tatcaaagta gaactcagat ttaaaatctt ttgttatatt attttttatg ttatcatcta
     5761 aatgaacttc ttgagatata gattttaatc taggaactag caatcctaaa acatccttag
     5821 aatcctcttt atcaaatttt aactctttag tctttgataa tatttgatat atttttgaat
     5881 aatcatgagc ttttttatgt gataataaat agatattgcc atcatataaa agtacatcat
     5941 tttttttagt aagtggaaca ggtagtgaat ctttagaata taagactatt ttatcttcta
     6001 tatttttaaa atcaaacctt acaggtagag tatcatttaa tatctttgga tgatattcct
     6061 ctttagaaaa aataagggta aaatccttgt acttaatagc atttaaaaat tgttttaaag
     6121 agcttgcatt tatctccata aatttttttg aatgtgatga agaaaagttg tatgaaagat
     6181 ttgtcccata ttcttctata atatctacta acttctcatc ttcatcagca aaataatgaa
     6241 tgttggggtt gtaagtaaat ttcttcccaa aatctaattc atatccatta agttttgaat
     6301 atgcaaattc agtaatgttt tttaaaatgt acattttatc aattcctatt ttaaattcag
     6361 caattgcttt atttgaatcc acatttatca atacatctaa attaaccctt tctcttttag
     6421 tattgtctaa caaagaaagt aattgcttgc ttaaatctaa gcttgtttta gaatttgtat
     6481 tagaaagctt atccttatct gcacctaata tatctattag aataggagga acatctcttt
     6541 tattttttat ttgttttaag ctttttatca caatagcaac tatatgctta catataaaaa
     6601 tacttttgcc ctttgattga cagtcagcgc aactacaatc attttcaatt atcttaaagt
     6661 gctttatatc tatagttatt gaattaaaat aaaagctata attatcttca gaattgactc
     6721 caccacgaaa tgtcaacagg ttattttgta ggtcatcaat atctatgtct atataatcaa
     6781 ctaattgatt agtataatat tttacacctc ttgaccatct tgatggttct gtatttcttc
     6841 ttattagttt atatatcata tctattttca tattattcac cttacctcac tgtatatatt
     6901 ataatctaaa tgcattcaat ttacactcta aatttaagac taaaattaag gcagatatga
     6961 atagctttta tctattacaa tatatgtgta aataacaatt tactattaga tttttattga
     7021 attatcttat aaactatgaa tgaaaaataa ttatataaat agattattaa taaatataaa
     7081 atcttattat taaaaatatt cttagaaatt gcactatata aaatataaag tatgatattt
     7141 gaataaaaag aatgtataat aagtataata atttgtattt attattggga aaggaaaatg
     7201 ttaatatgga tagttataga caacttaaag ctaaaatacc ctgtaaggaa acaggtatag
     7261 aaataaagag gactttatgt gatatttgtt ctccaggagt acattgtgga attgatgcat
     7321 atgttaaaga tggtaaagta ataaaaatag aaggtacaaa agaacatcca atgaataaag
     7381 ggttgttatg taccaaaggt ctttgcaata gagaatatat atatagagaa gatagaataa
     7441 aaacacctct aaaaagaatt ggaaaaagag gtgaaggaaa atttaaacca atatcatggg
     7501 atgaagctta tagagaaata gcagaaaaat taaatagagt aaaatctgag tatggagcaa
     7561 agcatgtagc tttttttacg ggttatggaa agtggtatag accttttttg caaaggtttg
     7621 catcttcatt tggttcacct aattatggta cagaatctag ttcatgttca acatcagcac
     7681 agatggctgg aaatgttgct actggaagaa actttacaat gcaagatatg gagaatgcaa
     7741 aaacttattt agggtgggga tttaatccat actactctaa atttttaaat agtgtgaatc
     7801 taataaaatt aaaagaaaaa ggtctaaaat ttataattgt agaccctaga ataacaccga
     7861 caactcaaaa actagcagat ttacatttaa gaattaaacc tggaactgat ggagctttag
     7921 ctcttggaat ggcgaatcta tttatacaaa aggatttaat agatagagaa tacatagaaa
     7981 agcatgtaca tggatttaat gaatataaag aatatgtaaa attatttgat ttagatactg
     8041 taagtagaat tacaggagtt tctaaagata atatagtaaa agctgttgag atgttgtcac
     8101 aaggtccatt ttgtataaat gaaagtgcag ctccaatagt tcatcatcaa aatggatttc
     8161 aaaactatag agcaatgatg tctttatctg ctataacagg taattatgat agaataggtg
     8221 gaaacatacc aaaacaagat tactttgctg gtaagatagg tggattccaa acactagaaa
     8281 aagagtttgt aaaagaagtt ctacctaaaa atataaatga aagaattggt ggagaaagat
     8341 ttcctctatg gaatgaaatg gtaactcaat ttcaagctat ggatttacct aggcaaatag
     8401 aagaaaagac accatatgaa ataagagctt tgttttgtca tgggataaat gctagaatgt
     8461 ttcctaattc agggagactt tttaaagcgt tgcatagtgt agattttttt gtgaatatag
     8521 atttatttat gacagataca tcaaaatatg cagatatact tttacctgcg tgtacatcat
     8581 ttgagagaga agaatgtaag agttacccag gagggtatat tacatatacc aaaaaagtta
     8641 ttgataaatt atacgattca aaatcagatg tagaaatact ttcagattta gcaaatgtta
     8701 tgaatataga tgatgattta ttaaaagcag gttatgaagc aagtataaga catatgttta
     8761 aaaagacgtg tgtagatgta gataaactta aagaaagtga tttaccatta aaatgtaaag
     8821 attttaagcc atatatagta ggctcatata caagagaggg gtatgatact agcacttcta
     8881 ggtttgaatt aaaatctaca attattgaaa aatataaaga ttttggactg gatgcactac
     8941 caacatatag aggatttaat gataatatag ataatgaata tactcattta ttgacatcag
     9001 gagcaagaat ttcaaatgca ctacattcaa gacttcatga catcaaactt tcaagaatat
     9061 taaaaaatga agctagttgt gatatttcca gcttggatgc aaaagaattg ggtgttatca
     9121 gtggagatta tataaaaata actactaaga taggaagttt ggatgtaaaa gtcaatgtta
     9181 ctgatagagt cttatataag aatatacata tgtatcatgg atatagagaa tctgatataa
     9241 atagtattat tgatgataat tttgaccctt atagtggatt ccctgcgtat agaagttgca
     9301 agtgtaagat tgaaaaaatg aatagataaa tggagagata taatgaaaag tttatttaaa
     9361 tttaataaag aattatgtac agcatgtgga gcatgtttgc ttgcatgtat agaccaaaat
     9421 gataataata tagacaatgg tgaacttttt agacatgtat ttacaataga ggaaggtgaa
     9481 aatatagaat atttttcatt agcatgtatc cattgtaatg agccaaaatg tttaggtgca
     9541 tgtcctaaaa attgttttaa aaaagaagaa ggattcgttg tattagacaa tcaaaactgt
     9601 atagggtgta aactttgtga aaaagcttgt gaatatggag ccttaattta tggaaatgat
     9661 ggtaaggcaa ataagtgtaa tggttgtatt gagagatata agtgtggtat ggattcacct
     9721 tgtgtaatgg tatgtcctac aggagcatta aaatttaata aataaattaa atatagagag
     9781 tgacttatac ttggtataag atatttttac aaaaagctat attgagtttg ttataataaa
     9841 aactcaatat agctttttta tggatatagt tggatactat aatgtgattt ttagtaatag
     9901 ttttttgcta ttggacaaag actgtaaaac atattaaact tatgttgttc aaagtcgaag
     9961 tataatataa taactagaaa attgatttat agtgttataa aagcaaattt agtaaggtat
    10021 aaaataagag gcaatctttt actacagtat caatatttgg gagttatgtt atggcagagc
    10081 cattaaacaa ttataatggt acaagtgatt caacctttga aaatgtatta tataaaaaat
    10141 taaaataaga ttaaaaagga gagaataaat tatgaaaaaa cttaaattaa ttattatgct
    10201 tgccttggta ttagcacttt taattacagg atgttcatca ggaaataata aaaataaaga
    10261 agaacaaaaa actataagag ttgcaacatc aggaacatat tacccttttg cttttaaaga
    10321 taaagataaa cttaaaggat ttgaagtaga tttttgggat gaatttgcta aacgaactaa
    10381 ttgtaaagtt gaatgggtat taactgattt tagtggatta tttggattat tagaagctga
    10441 taaagcagat gttgtatctg ctcaacttac tgcaacacct gaaagagaga aaaaatatgc
    10501 tttttcagat gcttataatt atagtggaac aaacattatt gttagagaag atgatactag
    10561 tataaaatca atagatgatt tagttggcaa gaaagtaggt gtgggtactg gagcagtagc
    10621 aaatgaaata ctaaaagaaa agtacccaaa tgatgaaata aaaatagtaa attattcaag
    10681 tgcaactctt aaagggaatt atcaagatct agaaatggga agattagatg cagtagttgc
    10741 gcaggatgtt gaggctttaa tagctataaa agaacaaaaa ttaaatctta aaatggtaga
    10801 acctccaatt caattcggag cctgttcttt tgcgattcaa aaaaatgaaa aaggtgaaaa
    10861 atggacaaaa gaaattaacg aagtaattga agagatgcat aaagatggga ctttgactaa
    10921 aatatctgaa gcctggctag gaaaagacat tactaaggag cctattaatg agtaatttta
    10981 attttgaatt ttgtctaagt atatttccat ttttgattaa agcattggga ataacaatac
    11041 taatatctat tgttgcattt tttttatcaa tattattagg tgtaatactt gcctttttca
    11101 cacagacaaa atcaaaagtt ttgcaataca taacaagagt gtatgtttca ttctttagag
    11161 gaacaccact tttaattcaa atatttttct tttactttgg atttccaatg atattttctt
    11221 ttatgaaaga ttgttcacct tatttagcaa tagtaatttg tttaggtgta aattcatctg
    11281 catatatgac tgaaattata cgagcatcta ttgagagtgt tgataaagga caacaggaag
    11341 catgttatgc ttttgggtta agtaaatatc agtcaatggt taaaataatt ctacctcaag
    11401 cagctacatc tgcagtacct cctattgtaa gctgtacact ggacataatt aaaggttctt
    11461 ctttaggtct aactatagga atacaagatt taatgggagt agctcaagta gaggctgctt
    11521 caagttttaa atattttgag atatatatga taacgatatt aatttactgg gcagtatcta
    11581 taatattgag taatatacaa agcaaagttg aaaaaagaat aggaaaggca tattagattt
    11641 caatatttaa aaatgggatg atgtagatat attattactt gtaaataaat ttttaatttt
    11701 agaaaaataa acttagtaat ctaaattatt gagaaaatat agtatcaaaa tggactgtat
    11761 aatcaagtta aaaaatagtt tttctattga aaaaataaat aaaattctat tgtaatttaa
    11821 tattcgacaa agttttataa aaaacaacat taagctaggg ggacttattt tatgattttt
    11881 aatatgaaat attttttaga attatttcca ataataatga aatcgttcaa tgtaactgta
    11941 attttggcaa taagttcatt agtattttca ttaattatag gtacaatagt agcattatca
    12001 tcttattaca aagtgaaagt attgaatcaa atatgtaaag tatatgtatc tatctttaga
    12061 ggtacgcctc ttatgccaca gttattcttt ttatattttg gactagcgta tatgaacgat
    12121 tttgttaaaa atatggaccc agtatttgca acatctatag tacttagttt aaatatggga
    12181 gcttatatgt ccgaaacgat aagggcatct atactttcaa ttgataaagg tcagatagaa
    12241 gcagcatatg taatgggatt aacaaattta caaacaatga aaaggatagt gataccacag
    12301 gccgtaagag ttgcactgcc atcattattc aataatttta tagatttaat taaagggtct
    12361 tctgtagctt ttgttgttgg agtaaatgat ataatgggtg ctgccaaatc acaaggagcg
    12421 ttgagtttta agttctttga agtgtatgga gcagttatgg taatttactg gtgcataata
    12481 actcctttag ggtgggggca aaaaaaatta gaaaagaaaa taagcaatta ttaagttatt
    12541 aagaaaatac ataaaagtat ttttatataa aaaatggtgt gaggagaaga gatatggtaa
    12601 aaagaaatat tataaaatgt ttagtggcag ttttatcgtt aacatttgta gttgctggtt
    12661 gttcaaaaaa agaggaagta aaggatgaaa aagttataac tgtagcttca tctaattcag
    12721 ctgacccata ttcttatgtt gataatggtg agcataaggg ttttgaagtt gatatgtgga
    12781 aagagattgg taaaagaagt ggatataaga taaaaatgga accaacaggt tttagttcta
    12841 tatttgggat gttagattct ggtaaagttg atgtggcagc caatttcttt ggaatgtcta
    12901 aagaaagatt agaaaaatac gatgcttcta taccttatgg ttctgattct gtatgtatag
    12961 ctgtaaaaga tggaaacaac gaaattaata aacttgaaga tttaaaagga aaagttgtag
    13021 cagtttcaga aggttctcaa gggcaacagg ttgcagctag tgttaataaa gataatttgt
    13081 ttgaagaaaa ggtttatggt gatggaacta atggtcttca agatttagat ttaggaagag
    13141 ttagcgcatg gattgatgca gaacttactg ttgtaggaga cgctaaaaaa gctaatatga
    13201 aggtaagagt attagatgaa aaattatcag ttactaatat agcatacttc tttaagaaaa
    13261 atgatgaaaa atctaaaact ataaaagaag atgtaaataa agcaatagaa gaaatgcttt
    13321 cagatggaac agtgaaaaaa atatctgaaa aatggtttgg aatggatgta agtgctgata
    13381 tacaaaaata aatactttaa aattaaaata taaaactgtg ataactatag ataagtatac
    13441 taaatttatc tatagttatc tgtgtattac aatgtattta gattagtctt tataaattaa
    13501 aatgagttat ggaggtcttg caaatgattg aaactaaaat aaaatccatt gcactaaaaa
    13561 atagagaaaa aatcataaaa ataagacgac agatacacag taatccagag ttagcattta
    13621 aagaatttaa aacatccaaa cttataaaag aagagttaaa taaacttaaa atagaataca
    13681 tagatgttgc tggtacagga gtattagcta ctataaaagg aaaaaataat ggtgggaaaa
    13741 ctatattgct tagagcggat atggatgccc ttccaatcaa agaggaaaat gatttagaat
    13801 ttaaatcaat aaatgataat atgcatgctt gtgggcatga tgcccatgtt tcatggttgt
    13861 taggaacagc aatgatttta aatgacataa aagaagaatt gaatggaaat gtaaaattac
    13921 tatttcaacc tggcgaagaa aaaggtggtt ctgatattgt aattaaagaa aatgtacttg
    13981 aaggtgtgga tgcattagca actggtcatt gttggccaac tattgaatca ggaaaaattg
    14041 gaatcgctag aaattgtgct atggcagcga ctaatacttt tgaaataaca ataattggca
    14101 agggcgggca tggagcagag cctcataatt gtatagaccc aattgcagtt ggaaatgcag
    14161 tatattcatc cattcaacag atagtttcaa gaaaaattga cccagttgta cctgtagtag
    14221 tgtctgtttg ttcttttaat tctggtgttt ctaaaaatat aataccagat gtatgcacac
    14281 ttcaagggac tattagagcg ataagcaagg aacaagtaat tgaagtaagt aaaattctag
    14341 aaaatatagt aagaaatgta tgtagagcat acggagcaga ttgcaaattt gaaaagagta
    14401 caggaggaga tgctgttata aatgacaaaa acatgataga gctaggaaga gaaagtgctt
    14461 gcaaaatact tggagttgaa aatgttgaaa taattgattt tcctgcaatg acaggtgagg
    14521 attttgctat ttatatgaaa gaaaaacctg gtttatttat gtatataggt gtaggaaata
    14581 aagaaaaaaa tataaactac agattgcata gtaacaagtt taatatagat gaaaaatgtc
    14641 ttagtatagc atcttcatta ttctcacaac ttgcagtaga ttatttatgt caactataga
    14701 gtacattcat aaaaatatat ttagcctatt tttaaattta atgctagtat attaattttg
    14761 atgtagaaca tattatttat tatatttatt taaaaaggtg gataacaatg ataaaagtag
    14821 aaaatttaca taaaagtttt aaagggacag aagttcttaa aggtataaat ttagagataa
    14881 atcaaagtga agtagtagct atacttggac catctggaac aggtaaatct acgttactta
    14941 gatgtttaaa ttatttagtt gagcctacaa aaggaaagat aatgattggt gatgtaaaag
    15001 tagatgtaga aaaagcaaca aaaaaagata tagccaattt aagaaaacat acatctatgg
    15061 tatttcaaaa ttataactta tttaaaaata aaacagcatt agaaaatgta atggaggcat
    15121 tgatagtagt acataaaaag agtaataaag aagctagaga aatatcttta aaattaatag
    15181 ataaagtagg tatgttggat aggaaagatt tttatccatc aaaattgtct ggggggcagc
    15241 aacaaaggat aggtatagca agagcgatgg cagtaaatcc aaatgtaata ttatttgatg
    15301 agccaacatc tgcacttgac ccagaacttg ttggagaagt tttaagcgtt ataaaatcgc
    15361 ttgcgaaaga aggaataaca atgcttattg taactcacga aataaatttt gcaaaagaag
    15421 ttgctgataa aattatattt atggataatg gtgtaatagc tgaagaagga actcctgatg
    15481 aaatatttaa taatcctaaa aatgaaagaa ctgcaaaatt tttaaatatc gttaggaaag
    15541 aagaagtatt tagttaaaag tgtataaatt aagtattaga agatatgtaa gatactccca
    15601 ttaaatttat aagttagata ataaatttaa tggaagtatc tttttattat tagatttatt
    15661 atttaattta aaattagaaa tgtaactatt tgaaaattct ttttaaaagt atatcaaaaa
    15721 tatcaagtac atgaaaaaat aatatttgac aaaatatcat atatgtttac aaaattaact
    15781 tgatggaat
//
LOCUS       DACTGW010000073        15623 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.073, whole genome shotgun sequence.
ACCESSION   DACTGW010000073 DACTGW010000000
VERSION     DACTGW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.073"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            164..1816
                     /locus_tag="I9573_001832"
     CDS             164..1816
                     /locus_tag="I9573_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HAT4796455.1"
                     /translation="MNNKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYKMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSDILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKIEYEITINAITGKILEFEIDD"
     gene            2113..2862
                     /locus_tag="I9573_001833"
     CDS             2113..2862
                     /locus_tag="I9573_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796456.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFDIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYAASFANRILIFKDG
                     HIIKELNKQPNNRHTFYELIIQEIAKLDTKR"
     gene            2875..4902
                     /locus_tag="I9573_001834"
     CDS             2875..4902
                     /locus_tag="I9573_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAT4796457.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFITGLVLGALSHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLVKLIRHEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLINGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            5046..6629
                     /locus_tag="I9573_001835"
     CDS             5046..6629
                     /locus_tag="I9573_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT4796458.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFL
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRNQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDIIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            6601..7221
                     /locus_tag="I9573_001836"
     CDS             6601..7221
                     /locus_tag="I9573_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4796459.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYEEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTNMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(7409..8335)
                     /locus_tag="I9573_001837"
     CDS             complement(7409..8335)
                     /locus_tag="I9573_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796460.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSENS
                     NAEIVTKNSDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNTYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENLDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            8945..9865
                     /locus_tag="I9573_001838"
     CDS             8945..9865
                     /locus_tag="I9573_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT4796461.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHFVDIYLPIEPF"
     gene            9951..12371
                     /locus_tag="I9573_001839"
     CDS             9951..12371
                     /locus_tag="I9573_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAT4796462.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLALLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKILINQGNLNTFGTNLVNDILSKTRHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSDSEIGDEDDYAVIETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            12371..13393
                     /locus_tag="I9573_001840"
     CDS             12371..13393
                     /locus_tag="I9573_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAT4796463.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWICKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            13508..14254
                     /locus_tag="I9573_001841"
     CDS             13508..14254
                     /locus_tag="I9573_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT4796464.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            14340..15590
                     /gene="cme"
                     /locus_tag="I9573_001842"
     CDS             14340..15590
                     /gene="cme"
                     /locus_tag="I9573_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HAT4796465.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHNIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLVMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
BASE COUNT         5791 a         2111 c         2309 g         5412 t
ORIGIN      
        1 tttatatttg tgatacaatg ttaaatataa aaaggaaaat tttgtattaa agaaatctta
       61 tttgttgatt ttgtaacttc aaactcagta tcaatttgat tttaagatta tattttatta
      121 agtatgctaa ataacaaatt tttaaagatt gtgaggtaaa aatatgaata ataaaaattt
      181 agctatatta atatcaggta tgatgttttt ttctagttct tcagcaattt ttgcagacaa
      241 tactacaaag caagaaagat taataggtaa aacaagatat gaaacagcag tagaagtaag
      301 taaattggga tgggttcaat caaaaacagc cattatagtt aatggtaatt ctattcaaag
      361 tgcgctttgt gcaaatccat ttgctaagct aaagaatgca cctatacttt tagtaaacaa
      421 caacagtatt gaaaattcaa ctaaggctga gcttaaaagg cttggtgtag ataatgtgta
      481 cattgtagat agtggaaata gcatttcttc taaagtagaa aatgaaataa aatcacttaa
      541 cataaaaata aataaaatag taggaaacaa tatatataaa atgtctacaa atgtacttaa
      601 agaaatagat aagataaaaa aggtagaaaa tgttgcagtt gtaaatggac taaagggatt
      661 atctgatgca gttagtatag cttctccagc agcaatacat aatatgccta taatattagt
      721 atcagatgaa aacagcaatg atagtagtat aaatttctta aacaataggt ctataaaaaa
      781 atcttacata gttggaggaa ataagatttt atcagacaat acagtaaaga aatatccaaa
      841 cagagaaaga atcagtggtt cagatagaaa caatactaat gcaaaaatta ttgataaatt
      901 ttataaagat aaaaaaataa ataatgtatt tgtagctaaa gatggtatta accaagaaaa
      961 tgcattagta gattcattat caataggagt attagctgct aaagaaaatt ctcctataat
     1021 gatatctaat ggtagccttg taaatagtca aaaggaattt attaaaaata aagatataga
     1081 tgaaataaca caagtaggag gaggatataa tcaaaaagca tttgaagata ttttaaaact
     1141 aaaaaatgtg aacataagaa ataaaactaa aaaaatatct ttaatacagg ctaaagaaat
     1201 attaaaacaa gctcttccag gaagcgatat tttagtttgt tattatgatg aagatgataa
     1261 tgaatatgac tgcaaagtta taaaggataa tatcctatat gagatagaaa ttgatgccta
     1321 ctctggtaaa ataatagaaa ttgaaaaaga tgatgactta aatgataata atttaggtac
     1381 acaaaatata tcattggata aagctaagtc tatagcatta gatgctgttc ctaatggtaa
     1441 tttaatctat tgtaaatatg atgctgaaga taacgaatat gaggctaaag taataaaaaa
     1501 taagaaaaca tatgaattag agataagtgc atttgatgga agaattgtag atattgacat
     1561 ggacaatgat tctgatgatg actttgatga taatgataag aataactcta atactaatga
     1621 caacagttct attattagtg ctgaaaaagc taaatcaata atgctagata aagtgcctgg
     1681 tgggaaaata gtaaaattta agtatgatga agatgacaaa gaatatgaag gtgaaataat
     1741 aaaaggaaaa attgaatatg agataacaat taatgcaatt actggtaaaa tattagaatt
     1801 tgaaatagat gattaataaa aaatatatat tctattaatt attacaagca ataaagccat
     1861 aaatattgaa tttttcaata tttatggctt tattttattt tttttaattt aaatctacat
     1921 tatgccatta tatatctatg cactctttta aattaaaatc ttgtccatca tcccaaacct
     1981 aataaagtta gattttaaac aatatattgt gatataagta atttgtgtag aattattgcg
     2041 tgatttactc atgaagaaac aataaggtta atatataatt tgactatatt aagacgaaag
     2101 gagacatata caatgaatac aatattaaaa gtaaataaca taactaaaac ttataataaa
     2161 gggcaacagc cgagtttaga taaggtatcc tttaatgtat cagaaggtga atttataggt
     2221 ataatgggag cttctggctc tgggaaaaca actctgctta atgtattatc tacaattgat
     2281 aaaccaacaa gtggagatat tttcataaat aatataaatt tgaaatcgct taatgacact
     2341 tgtgctgctg attttcgtaa ggataattta ggctttatct ttcaagagta ttttttacta
     2401 gatagtctta caattttcga gaatatttca gtacctctta cacttcagaa actcaatcca
     2461 gataaaatag aaaacatggt aaagagtcta gctgaacgat ttgacatagc ttctcaatta
     2521 aataaatacc caaatgaatt atctggtggg caaaggcaga gagcttcagc agttagagca
     2581 atagtaaaac atcctagtat tctctttgca gatgaaccaa caggagcttt ggattctaat
     2641 tcagctactg aactactcca ctcactacag gaagcaaatg aggagttgaa aacaacaatt
     2701 ttaatggtaa cacatgatgc ctatgcagca agttttgcaa atagaatttt gatttttaaa
     2761 gatggtcata tcattaaaga attaaataaa caaccgaata atagacatac attttatgag
     2821 ttgattattc aagaaattgc aaaattggat actaaaagat aggaggataa tttaatgaac
     2881 attttttata tggcaataaa gaatcttaaa aaaaactttt cattctattc tctatatcta
     2941 ctttcagtct cttttgttat tactgtattt ttcgcattta cttcattctc tgttaataag
     3001 ataatgctag aaaaaatttc aactgatggt agagttgaat ctatgtgtaa tgttatttcg
     3061 atatttttga tggcatttgt catcttctat atggcatact caaatcgatt cttcttgaga
     3121 cgtcgtacaa aagaattagg catatatgct ttacttggct atcgtaagtt tacaatatta
     3181 tcgctactta cctttgaaaa tgtattaatt tgtagtggag cttttattac tggattggta
     3241 ttaggagcat tgtcacataa gggtattgtc tttggaatta cagcactact taatttatca
     3301 attgataatt cacagatacc attttttaat ataaatgcaa taaacaaaac aattatcttt
     3361 atagcattag ttgttttagt cttagctata tcaaatggta aatttcttta taagacttca
     3421 cttgtaaaat tgataagaca tgaaaaaagt gcagaaaaaa aattaaagtt cagaaaaatt
     3481 ccagccttta tgggatttat tatgatttta agtgggtatt gtttatcact agatattctt
     3541 cgtggagcta aatcagtatg ggtaagtatt ggattttatc aaatgggctt gataactatg
     3601 attatcattg taattggtac agttctcttt atcaattcct ttcttccata tattatgcaa
     3661 aagagtaaaa aaaataaacg tactttctac acagatatga agataatagc tattccaaac
     3721 tttatctatc gtatacgttc aaactctaaa actctaatta tgcttacatt actctctgct
     3781 actactttga cagtttcaag tgttatggca ctgtccttat actaccctat tgcatctgtt
     3841 tcgcgtattg caccttcaga gatagaattt agagtagaaa aaaataaaca aatagaagat
     3901 gtaaaacaaa ttgtcagtca atatacgtct aatacagatg atattatttt tacacagaca
     3961 aatatttata aggtaaaatc ttcatcaaaa gaccttccat ttgaatatga aattggtaca
     4021 tctaagaata atttatctaa tagtagttct tctaaagaag ttatttcaag agaatcagga
     4081 tttgaatgta tatctttttc tgattattat actttattaa aggcacaaaa tcgaacaaag
     4141 ctactaaaac aacttacccc tttaagggat aatgaatgta ttttaatgaa atatcaacca
     4201 aataatgatg gatatagtga aaatggaacg atttatccat tacttattaa tggtaaagaa
     4261 acacctgtag ctgtaaggga aacatcatta aacaatgcta tttcttttgc caatagcgtt
     4321 ggaacactta tcgttaatga tagtatatat gagcaaatag cacaaaataa attaccaatg
     4381 acaagcattt taagtattaa tggtaaatca attaaaaata atgaaaattt atatagtaat
     4441 atttcagatt atttagatgg aagcccttat ttacaaggat attctcacag gataaatgat
     4501 ttattatcaa ttaatagttc tacatttctg ttaatcggat ttcttgtggt attattcttt
     4561 attgctgttg gaagtatttt atactttaac aatatatcag caatttcaga ttcaacatct
     4621 gattataaga ttttacagaa aatgggttat gataatatac aaattaaaaa aattattaaa
     4681 aaacaagtat tgccattttt taatattccg tttttacttg gtatattgga ctgtatattt
     4741 gctaccttgg tttataagac tgctttgatg cagaatttac ttggaaattc attttcacaa
     4801 tacatacctg tgattattgc aattttctta acttctgtag tgtatttgat atattatata
     4861 cttactatta aatcctgttg taaaattgta ttaaaaaatt aatatttacc aaatcgtcaa
     4921 gcttagaggt gtgacgattt ggtatacttc atttataagt atttgatata atttaaaatt
     4981 atagattctt aggtttgatt tagtcataaa attaatataa ttaactcata aggaggtgta
     5041 taagattgac aaattttaat ttaaaaatac tatttttagg aaactacatt ctatcacttc
     5101 tcttcatggg aatatctaca agcactgaaa cttatatgaa tatccaaagc attattccct
     5161 taataatttc aacattatta ctcatagaca tcacatatat aacctcttgt aatgtccgag
     5221 aaaataacat tatttcatta ttttgtggat tactagcact agacagttgg tatttactct
     5281 tatcatttgt aaccacacca ataggcacta ttatttttaa aatattaagc acagttatag
     5341 tgtatgtctc tattaaattt atatttttat ttttgtttca aggtagtgga tacaatttcc
     5401 gtaagataac agatatttta ttgcttatca cctgcattgg tgcaattgta ggagcattta
     5461 tttctaacag aacgtatgct tgtatgtacg gaatacagtt tgttgttagt ataataagct
     5521 ttttatatat tattttttat catttgaaaa gggttgtttt tgtcataaaa agcgaaaaaa
     5581 agcctatatc gatttcagta tttataacaa ttataggatt ccttacttat tattttttta
     5641 cactaagaat atcaaatcat attggtaatt ttggaatcta tctaactgta ttggtatttt
     5701 ttatgagtat tcatggtatt gcacttaaag aaaatgatag ttttcctatt tcaacagttt
     5761 ttaacataaa acaaagggct atatttcttt ttataagtat tttagtaatt agcttggtcg
     5821 tactatttgc caatgggaat ttttctataa tattaatagc tataaatgtt ttatttatgc
     5881 ttattttcct ctgtaacatt attttggagt ttaaccttag agatgaaaaa aataaagtag
     5941 ttaaaaatag caagtatact tttgcattag aaaaattaca acaagaagaa caactaaaaa
     6001 cagagttttc taatttttta catgatgaca tattacagaa cctgctttca ataaaaaaca
     6061 tgatgagtaa gtcatatcgc ccagaagttc aagatattat atatgaaaca cttaacaatc
     6121 taaatacatg tataagaaat caaatgcaag actatcatcc tgttatttta aaaaatttaa
     6181 cagtaaaaga aaattatcag aatttaattg aaagtgtttc ttcatccttt ccacaaaaaa
     6241 acatttgtgt ttcatttaag tgttctgata ctttattctt agttgaacca tatgatatat
     6301 tgatatatcg actgattaaa gaattattga caaacgtgta taaacattca aatggtagcc
     6361 atgcatggat agctttagca ctagaaaaaa atataataaa attaaatatt aatgataatg
     6421 gtgtgaaaaa tacatctttt aaaacacctt tagatataac taaaattact tttgatataa
     6481 taaaacacaa agggattata tcaattaaag aacaagtaga aaatctagga ggaacgataa
     6541 gtatttctga taatatacct catggagttt gtgtacagat tacaatccct atgaaaggag
     6601 atggttctta taaatatttt gttagttgat gaccatatgc tttttgctaa aagcttgtca
     6661 attgcactag aagaatacga agaaatcgaa cagttttatt ccacacagga catccataat
     6721 ctttcagaaa tgattcaaga taaaaaaata gacattgtct taatggatat aaaccttaga
     6781 ggcttaagtg aatgtgatgg cttaaaagta gcttataatt tattagagac taattcaact
     6841 attaaaataa taatcttgac tggatacgac ctcccagttt ataaacatga ggctaaaaaa
     6901 ataggtatct atggattcct aaataagaat attagccctg atagtttaat tgattcatta
     6961 ttaaaagtct atcaaggggt aacctgtttt ccatctgaaa atactgaagt aattattgaa
     7021 gatttaacta atatggagaa aagaatttta cagcttatct gtagtggtaa aaaaagaaaa
     7081 ataattgctg atgaattgta tataagtgaa agaacattat caaatcattt acaacacata
     7141 tatgacaagc tagatgtttc atcatcagtt gaggctatta caaaagccat taaaatgggt
     7201 tatatttctc cagtatatta aaaaagtgag tgtctcaaaa tgaactttct agctcataag
     7261 gcactctcct ttgtattaaa tcaaatatat tttcatcatt taaacaatta agaagaggct
     7321 tctctctatt ttgagacaac tccattttaa tacactattt atatttatat ataaacatat
     7381 tttaaacaac taaaaagcaa ctaatatctc aatacatatc ttcataacac atatcaccat
     7441 aattacaagc agataaagaa ctctcctcat ttaaatacga ctcaaattca tacccaaaca
     7501 gaattgttgg ttctatatat atttccatat ctgtatatct ccatcttctt acctttgcct
     7561 ctataacctt gtaaaaatct tctaaactat acccttcttt taatctttta tctataagtg
     7621 ccttagttat actcaaatca gctttaaaat tttttcctga ttcactattt aattttctta
     7681 taataatttc atgtatgctt tctggatttt tataagtaga atcttcagtc aaatcttcat
     7741 ccaaattttc aaaggtattt ttatttaaat tctttttatt taaattattt tttaataatt
     7801 cttttttctt tgtctcattt tctgtttcat acccagtatg aattatcaaa tcataatctt
     7861 tgtctatact ctcttgattt atacggctgt atgtgttggt attactgttc ttgaacaact
     7921 catctctatg tttatatttc attgtttttt ggttattgtt aatagttgca tcaatgtatt
     7981 tctctatatt tttgaaaata tcactattct ttgttacaat ttctgcatta ctattttcag
     8041 aacaaacata actatataca gaacaacaat ttccctttac tcccctactt attaatctca
     8101 atatacctaa atctataaat tcatttacaa gtctacttat tgtagatata gatacatcaa
     8161 ggtcagatgc agctttcctt actgtcatca taaattgtcc tcttggcaga gttcctttta
     8221 atctttgtat attttctttt ctcattacat agtgattaaa ccttattcta tttacatctc
     8281 ttttttcatt aaaatataat actgctgaat ttaattgata aaaactatta ctcatattcc
     8341 cactcctcca atttaaatag ataatttgtt gtaaagtttt tagattttta aataacttat
     8401 taaatgatta tttttattaa aaagtattct ttgatatttt cattttaaag ttttaagtat
     8461 aataagttaa tagtgtagat ttaacttttt tatttaaaca ataaacttaa tattattttt
     8521 aatataataa ttttattaaa tttagtatgt aattttttaa tcttatttta ttccctctat
     8581 actcttatgc taaataataa ctttgataat atttccctaa cttatgtttc aactggttta
     8641 tattgcaatt tatttaatta cagcttattt gcataataat tcgtccatat cataagttgt
     8701 gtatttatat aaatacacaa ttaaaaataa atcactatgg tatactaatt attacgaaga
     8761 ctatacttat atgcaaaaat aaatataagg catcttatat cttttattaa ttataaatat
     8821 cttttatcaa ttataaaaaa ttaaacgcta tcttatttat cataaattgc aaaaacataa
     8881 aattttatta ctatattgct ataaattaat taatacacat tttacacaat aaggagggct
     8941 atatatgaca aatgctaaaa gtaaaaaaga atatctaaat cgtatttgca aagtgcaaga
     9001 ctatatagaa gaacatttac atgaacctct ttctcttgat gaactttcta atatagcagg
     9061 tttttcaaaa tttcattttc atagaatctt caaagcaata gaaaaagaaa ctttggcaca
     9121 atatgtaaat aggctaaaac tagaaaatgc aactgctttt cttattcatc gaactgacat
     9181 gactgttact gatattgcac actacttcgg ctttactgat tcagcagttt tctcgagagc
     9241 atttaaaaat tactataaaa taagccctat aaaatataga aacaattata gcaagaattg
     9301 caaagaacca tataaaattt ctcagtacaa tagaagcata tcaaaaccta aatgtaagaa
     9361 tactagagag gttaaaggtg aaattgaaat tctagaactt gataatatga atactattta
     9421 cactagatat actggttcat atggtaatct aaccagtact ttcccaaaac ttttagaacg
     9481 gttattcaca tatgcaagtg agcagaatct acttggactt gaaaacacac aaatattagc
     9541 catttatcat gataatccac aatttacaca ggagcatcaa ttaaggacta gtatttgcat
     9601 gaccattcca gataacattg ttgtcaaaga gaatgacaat cttggcaata tgataatccc
     9661 ttctggaaaa tatcttgtgg gacattttga tatttcaaaa tctgaatata gtgatgcgtg
     9721 ggattttatg tataatgaat ggctatcaaa tggtaattat aagctacgtg cctccttccc
     9781 ttttgaagta tatcttaaca acccacacaa tcaccctcaa aatagacact ttgtcgatat
     9841 atatttacct atagaacctt tttaataacc tcacctaatc tattcttgta ttaaggatta
     9901 atatatccct attttacggt ttgataaaat tgataaaatg aggtgaacca atgaaagaaa
     9961 taaaaattaa tggagcaaag atacacaatc ttaaaaatat agatgtttct atccccaaag
    10021 ataagcttgt tgttgcaaca ggtgtcagtg gctcaggtaa gtctagcctt atgtttgaca
    10081 ttgtatttga ggaaggtcgt aaacaatatc tgcaatcttt aggtatactt gcaggtattg
    10141 atagtgagga taagtttgat aatattcaag gtttagctcc cactatagct gttcaacaaa
    10201 atattattcg ccagagcaat cctcgttcaa cagtaggcac aagaaccaat attctcaaca
    10261 tgcttgcact attgtattca gtagaaggtc aaataatgtg tactatgtgt gatactcctg
    10321 taagcgataa tctaatttgc aataattgtg ggtatgagga agaacgatta agaccaagtt
    10381 atttctcata caactcttca gatggaatgt gtatgaagtg ctctggacat ggtgcatatt
    10441 ttgagattaa tattgaaaaa ttagtccttg ataagcatga taccctaaaa caagtcttag
    10501 atagagcaaa gattacacca ggatatttga gagtattttc taaaaaattc aacgattatt
    10561 tagacataca attcttacaa ctacctgaag aaataagaaa tgaagtactc tatggtcatt
    10621 atgaaaatgg taaaaaaagt tcctctttat caaaggtatt tcacaatcgt tacaaaaggg
    10681 gtgaggattt aaatggagtt tatactatga ctgcctgttc agattgcaat ggttttaaaa
    10741 ttggtgaaga agcacgtaga gtacttttaa acggaaaaca tattggagaa cttggaaaaa
    10801 tgacgatttt agaaattgat gatttcctta aaatcttaat aaaccaaggt aatttaaaca
    10861 catttggtac aaatcttgta aatgatattt tatccaaaac tcgacattta atagaatcac
    10921 gattaggtca tctatcctta taccgagaaa tgtcaacttt gagtggtggg gaaatgcaaa
    10981 gactattttt aaattcacat ctcgattcag agatggactc attaatttat gtgcttgatg
    11041 aaccaacagc tggattgcat gaatcagaaa aaatagacat cttaaaatca ataaagaaac
    11101 ttaaagattt aggcaatacg gtcatagtag ttgagcacga taaaaacaca attgaaatgg
    11161 cagaacatat tattgatatt ggtccaaaag ctggtgttga aggtggtcaa ttagtatatc
    11221 aaggagattt agaaggtcta cttcaatccg acaaatcact tactgggcag tatctatctg
    11281 gaaaatatcc aatgcctaat agaacaagca ataaaaatat tacacatact gataaaatac
    11341 cttgtataac tattcaaaat gcaaatacaa ataatcttaa aaatgtaact gtatcattac
    11401 ctttgggtgc tatggttgga atatctggaa aatcaggtag tggcaagagt tcacttatat
    11461 cagacaccct acttcctctt cttagaagcc aatttaataa ccatactatc aataatcaaa
    11521 taaattcttc agatagcgaa ataggtgatg aagacgatta tgcagttatt gaaactattg
    11581 cagatagact gattggtact aaacatattt ctggatattc agagatatct caatctccta
    11641 ttggcaaaaa tatgaactca actcctgctt cttatattgg tatatgggat aagatacgta
    11701 cactatttgc aggtcagcca gaatcgttag aacgaggatt tacagctggt catttctcat
    11761 ttaactccaa aggtgcttgt cctaaatgta gtggtagtgg atatgaaaag gtctggttag
    11821 gaaataatct aagtatagac catatatgca gtgaatgtca gggaaaaaga tttaatgatg
    11881 aatctttatc tattaaatat aaaaataaaa atatacatga tattttaaat atgagtgttt
    11941 ctgaatctgt acatttcttc aaggatattc caaatattgt ttcacactta aatgtattag
    12001 aacaaatagg tatggggtat ataaaattag gccaacctac accgacttta agtggtggtg
    12061 aatcccaaag aatcaaactt gcaaaagaaa ttggtaaaaa acgaaatggt aatatccttt
    12121 atgtattgga tgagccaact actggtctaa gtctatatga tacttctaag ttgattcaat
    12181 tattagatga attggtggaa aatggaaatt ctgtaattgt tgttgaacat gatatagatg
    12241 tattaaatgt ttgtgactgg attgttgagc ttggtccaga aggtggtgac aaaggaggtt
    12301 atataattgc agaaggttct ccaaaaactc taaaagaaaa tccaaaatct ataacaggga
    12361 ggtatcttta atgaataaaa acaattgctt agatttaata tttgaaaaag tagacccttc
    12421 aagtgttggc atgagttcag aaaagctctc acaactggaa cctttaataa tctcacaata
    12481 tagtaatata aatggaataa ttattgcaag aaaaggaaaa gttgtctttg aaaagtacta
    12541 taatggatat aatgctgatg atacttttca tgttgcatca gttacaaaaa gtattatatc
    12601 agccttaata ggtattgcaa tagataaagg atatataaaa gatgttgacc aaaaggttgt
    12661 tgattttttc cctgaatata cttgtagtcc ttctgaaata tgtaaaaaag cagtaactat
    12721 acgtcaccta cttacaatga ctgcaccata cccttttaaa aatatgactg agtctttaga
    12781 aagactttgc aaacaaaaag attggataaa atataccttg aatcaacttg gtcaaaaagg
    12841 cgaaattggt actttcaaat attcttcagc tggaacgcac ctactttcag ctattataac
    12901 aaaaactact ggaaaatctg ctcgtgaatt tgctaatgag tacttattta aacctattgg
    12961 tatgaaagtt ataccagatt atgatatgaa agattttggt tttgaagatt tctttggaaa
    13021 aaatcttaag ggttgggcaa aagatagaca gggttattct ataggtggct ggggacttac
    13081 attgagtgct agagatatgt tgcgatttgg ttttctgtat ttaaatgatg gtatttggaa
    13141 taataaacgt attatatcta aatcgtggat tgaagaatcg gttaaaatga atagtaatga
    13201 atacggttat ctgtggtgga tatgtaaaaa agatggtgtt ttgtggtatt cagcaatggg
    13261 aactggagga aatgctattt gttgtattcc acaatatgac ttagtaatag ccataccatc
    13321 taaaatagtg tttaaacctc gtgatagatg ggagtttatt gaaaaatata tacttacttc
    13381 tataatagat taatcactct tagataaact aattactctt agataaccaa accctccact
    13441 ataacaccag acttttgttt ggtgttatta tgactaatac tatatatcta aaaggagttg
    13501 aatactaatg gacacttaca cgacatcaga ggtagcagat ttaattggtg tacatcctaa
    13561 tacagtacgt ttatatgaag aactaaactt aatacctaaa cctaagcgta ataccaatgg
    13621 ttatcgtatc tttaatgatt ttcatataga acaatttaga tttgctcgta cagccttgaa
    13681 aattgaagtg gtacaaaggg gattaagaaa agatgccctt gagattgtaa gaacatcagc
    13741 gaatagggac tttgataagg ctattagtct tgtaaatcaa tacttgcaac aattagaaat
    13801 agaaaaatct aatgccgagg aagcacttac tattgttgca cagattttaa atggaaatac
    13861 tccagaaaca aataaactct ttttaactag aaaagaaact gcagaacact taaatgttac
    13921 aatagatact ttaagaaatt gggaaatgaa tggtttgtta aaagtaaaaa gaaaacaaaa
    13981 tggttatcgt gtatatactg atgaagatat aaaacgactt aaaattataa gcgcacttcg
    14041 ttgtgcaaac tattctcttt cagctatttt acgaatgtta aatgctttat cttctaatcc
    14101 taatactaat attaaaactg tccttgacac tccaaaagat gatgacgaaa ttatctcagt
    14161 gtgtgacaaa ctgataactt cattgaaaga agctgaacat aatgcaaaaa ccatgaaatc
    14221 tcatcttcaa attatgaaaa aacaattttc ctagccctcc actctaactc cagactttga
    14281 tttgtgtgtt atccttaaga gcaagaaaaa ttcaacacac aaatcaaagg aggaatagga
    14341 tggaaaacaa aaatactaat tctacatgga agaaaaaagt cattcttttt ttagcaagtc
    14401 aatatatttc cttatttggc tcatcacttg tacaaatggc aatgatatgg tatgtcacac
    14461 ttcagacatc atcaggtgta tgggtaacag ttttaactgt atgctccttt atacctcaaa
    14521 tgataatttc tttttttgct ggtgtatggg cagactacta caatcgaaag gtactcataa
    14581 ttgtatcaga ttcaacaatt gctatatcta cattagttct aattctattt atgatgtcat
    14641 caaatggcac aacaagtgaa ttaatagcca tatcagtaat atctgttata cgttcactag
    14701 gaagtggtgt acaaacacct gctgtaaacg caatgattcc tcaacttgta ccagaagaac
    14761 atttaatgcg ttttaatggt ataaatagta gtctacagtc tatagtacaa tttatagtgc
    14821 ctattgtagc tgctgcaata cttggatttg gtaataaaat aaatcaagtt cttttaattg
    14881 atgtatttac tgcaattatt gctattgtat tattgatatt catcaaaatt cctaaacata
    14941 atataccaaa acaaactgaa aaagtctcat tttttgcaga tatggtatca gggataaagt
    15001 actgtttttc agataagttc attagaaaag tgcttttcac ttatggagca ttcatgcttc
    15061 tttgtgttcc atcaggattt cttataggac ttcttattga acgaacattt ggcaagggtt
    15121 atatatacct gtctattatt gaaatgattg gatttttagt tatggtttta ggtggattga
    15181 ttataggagt ttttggtggt tttaaaaata gaaataagac cttttttcta gcaatattat
    15241 cctatgctat tttttcaatt attctaggtc ttgtaacaca agcttggcaa ttttttatat
    15301 ttatgttttt tacgaatttt tcaattccaa ttattcaagc atcagctatg actatgttgc
    15361 aagagcatgt agcaccacaa atgctaggtc gagttcttag cttacctatt attatatata
    15421 ctggttttat tcctcttggt atgatgattt tcggaccaat ggcagatgta atttctatta
    15481 ataatttaat aatcgtagca gggattatac ttttattttt tgctttggct atcccctatt
    15541 caaaacaatt ttacaagcaa ggaatatcta agtcaaatga atccgagtaa tatatttaaa
    15601 tagtttaact cgatttatct ggt
//
LOCUS       DACTGW010000074        15536 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.074, whole genome shotgun sequence.
ACCESSION   DACTGW010000074 DACTGW010000000
VERSION     DACTGW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.074"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(123..629)
                     /locus_tag="I9573_001843"
     CDS             complement(123..629)
                     /locus_tag="I9573_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAT4796466.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            complement(1000..2028)
                     /locus_tag="I9573_001844"
     CDS             complement(1000..2028)
                     /locus_tag="I9573_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HAT4796467.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDINKKMDTIDKLKDI
                     EEGYFYLLEDVYLDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            complement(2076..2936)
                     /locus_tag="I9573_001845"
     CDS             complement(2076..2936)
                     /locus_tag="I9573_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAT4796468.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKSI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            3215..3424
                     /locus_tag="I9573_001846"
     CDS             3215..3424
                     /locus_tag="I9573_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HAT4796469.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            3424..3690
                     /locus_tag="I9573_001847"
     CDS             3424..3690
                     /locus_tag="I9573_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HAT4796470.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            3719..4291
                     /locus_tag="I9573_001848"
     CDS             3719..4291
                     /locus_tag="I9573_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAT4796471.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(4525..5715)
                     /locus_tag="I9573_001849"
     CDS             complement(4525..5715)
                     /locus_tag="I9573_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAT4796472.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENVENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(5975..6406)
                     /gene="dut"
                     /locus_tag="I9573_001850"
     CDS             complement(5975..6406)
                     /gene="dut"
                     /locus_tag="I9573_001850"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAT4796473.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            6519..8747
                     /locus_tag="I9573_001851"
     CDS             6519..8747
                     /locus_tag="I9573_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAT4796474.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEYEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(8858..9343)
                     /locus_tag="I9573_001852"
     CDS             complement(8858..9343)
                     /locus_tag="I9573_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT4796475.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            9595..10818
                     /locus_tag="I9573_001853"
     CDS             9595..10818
                     /locus_tag="I9573_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAT4796476.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTKESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNANNKFKAGMSAQVNLSIEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            10822..13956
                     /locus_tag="I9573_001854"
     CDS             10822..13956
                     /locus_tag="I9573_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAT4796477.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            13990..15288
                     /locus_tag="I9573_001855"
     CDS             13990..15288
                     /locus_tag="I9573_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HAT4796478.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINNVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNQDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
BASE COUNT         5705 a         2273 c         2227 g         5331 t
ORIGIN      
        1 ttttataata gtatttattt aaaattaata tttatttagt tagtagcatc tacttagata
       61 tcagataaga aagcacagtc atttacttat aataagttct tttcatatat tttttattat
      121 aattattctt ttacaagaac ttttataatt tctgaaatgt acatagtatg atagtctttc
      181 tctggatacc attttttatc attttcttta gcatcaaagt tttctggtgg catattacca
      241 tgatatagct ttttacatac tataatcata ttagcttctt caaaagctac cgtatcatcc
      301 acaaaatatg gtgtaagtcc tgctttttct attttattta tatctctacc agaaatagta
      361 ccacaaatat ttaaagcctc tctatacgtt tcatcaaaga aggcaactgt aaatgtatca
      421 ttactatcta caaattcttt tgtatatctc tgtggtctta tgtaagttgt aacaacattt
      481 ttaccccaat agactccaac accaccccag ctagcagtca ttgtgttaaa cttttctgaa
      541 tcaccagctg ttataagtaa ccattctttg ccaatcttag taaatggatt aaactgtagt
      601 tcctcaattt ttacttctct aaaacccatg acttcatcct acccttcatt ttttaattat
      661 ataaattaat tgttaaaaat ttgaatatat gtactcttat aatactgact tttgattgtc
      721 ctacataatt catgtaaatt ataatacaac aaatcaatta ctttcgcaat aagtagtttt
      781 catattttat aagtgtaaaa tatagataaa atatattgat tataaagtat ataatatatt
      841 tttaaaccta aagcaatata caataaaaaa atgtagaaag taagtatctc tctacatttt
      901 ttattatata ttatttttat attaatttaa ttttatcttg attcagtttt ataaaaaatt
      961 gttataattg tcaagttcta ctctattatt tgtaatttct tatatcttgt taacaacatt
     1021 taagttatta catttattta tatatatttt ttcattatct aagtcttcat caaacatttt
     1081 tattatattt tttatctcat acttgtactc atcttcattc attctataaa gcattaaatc
     1141 ttctaataca tctttcatgt cgaggtacac atcttctaat aaataaaaat agccttcctc
     1201 tatatctttc aatttatcta tagtatccat ttttttattt atatctttca taatttctaa
     1261 attttgtata tttacatcta tgattgttaa taagtacatt ctcacagttt ttattatttc
     1321 aacagaaaaa tctacaatat tataatttcc ttctttttta tataatgact ccttaaattt
     1381 gttaaaacaa ttatctaaat tagaatttat atcgttaaat ggtggtctta ttatgtcacg
     1441 tattattaaa agattttgta tatctacatt atttgtgatt gcctcatcca aaataaataa
     1501 attatatgca tcatttaatg ggtcatgggc atctccacta aactctaagt taacaaattc
     1561 aagtaaattt ttcaatgata tatagttcat acatctcata ccacaattta tatatttttc
     1621 ttttatatct acaaataaat ctctaatatt atttacaaac cttggatggt tatttttttt
     1681 agcacttctc atatctgtgt tattgaaaca tgtcaaatcc aaattaccaa aagtatatat
     1741 tcctttaata tcagaaaata tgccaagcca ttttttaaat tttctcatga cctcttcata
     1801 acttggtgca ctcttcaatt cttcagaaga tatatgtgtt aattcttgta tatgaggata
     1861 taactcctca ttacttactg gtcttattaa agatttaaat ttatcaatat tttttgtttt
     1921 ttcatcatac ataactgctc cgattgcaat tatatcagaa ttaaatactg ctctttttga
     1981 tttagaattt ggcatattca tttcaaaatc tatatatact cttttcaata taaacaccct
     2041 ttccaattgt ttatagtgat tctttttaac tatttttatt caaaattttc taagaaagaa
     2101 tgtttctccc catctttttg ccaaccaagt actgaatcaa gatttacatt ttctgctgcc
     2161 ttaaatatag atttttctac atttttaaaa tttgattcta agctctttat taagtctttt
     2221 atctcatatc ttttattacc tgttttttta gcagcaacca tacatgcaca atttaatggc
     2281 catataccac tattttgaat aaatcttata atatgttctt ctcttatata ataaagtggt
     2341 cttataattt ttataccttc aaaattagta gaattaagtt taggaagcat tgttttaaaa
     2401 tttccagcac acaataagtt aagcattgtt gtctctatta catcatcata atgatgacct
     2461 aatgcaagtt tgttacaccc taattcttct gcctttgaat ataatgctcc tctcctcatt
     2521 cttgcacaca tatagcatgg ataatctttt gctatttcat ctgctatctc aaatattctt
     2581 gaatcaaata aatgtattgg tatatttaga tactcacaat tatctataag taattgtctt
     2641 atatttgcat ggtatccagg gtccatagca ataaattcaa catcaaaatt aacttgtcca
     2701 tgttttttta actcttgaaa cattttagcc ataagaatac tgtcttttcc accagaaatc
     2761 gctacagcta tcttatcccc ttcttcaacc aatttataat ctcttattga cttcataaac
     2821 ttagaccata aattttttct atatttcttt attatacttt tttctatatc tcttactgga
     2881 agtctttcct caaaaggaac tataatttca cagccttttc ctgatagttc actcatctaa
     2941 tcacctcgta tatttatgta tatttatgtt aataatattt tacaaaaaag tctaccttca
     3001 tccacaaaat actataattt ttactttaat tttactaaat aatttatagt ttacaataac
     3061 acataaactt ataataacat aaataacctc tttcttttat tatctttttt atttttctca
     3121 tttaatattt tattattagt aacgattttt aaatgattga atattataca ctagatagag
     3181 ttacctatct atttacaaca aggaggtttt catcttggaa aataagacta gagatatcaa
     3241 aaaaaattgt ggtccatctt tggctagacc atacattaat aatcaaattt tcacagaaat
     3301 gtactgcctt tctgatgcat taaaatttgg aactatattt ccagaactaa acttactaga
     3361 gtctgaaatg tacaacaaag aattatatgc taagccaaaa aaatatagag gaggtagaag
     3421 ataatgaaag actcatctag agctgaactt atgagaaaag ttcaggaaac atcttttgca
     3481 tgtgttgata tgaacttata tttagacaat caccctgatg ataaaaatgc tatcaacact
     3541 tataattcat tatgtaatca atttgcgcaa gctagatatg cttacgaaaa taaatacggt
     3601 cctttaacta actttgggta tgctccaagt agatgccctt ggcaatgggc tgaccaacct
     3661 tggccttggg acagagaatt taattattag acatcaagta ttggaggatt gaaaattcat
     3721 gtggatatat cagaaaacta tacaacaccc agttaatata aaaacttgtg accctagaat
     3781 ggctaaattt cttataactc aatttggtgg gccaaatgga gaacttgctg catctttaag
     3841 atatttaagc caaagatata caatgcctac tggtaatatg cgtgcacttt taacagatat
     3901 tggtacagaa gagctagctc acgttgagct tatatgtact atggtttatc aattaacttc
     3961 tgatgcaagc ccagaagagt taaaagctgc aggtcttggt tcaaactatg ctcaaaatgg
     4021 atatggaatt tatccaacag attcaaatgg tgttccattt gatgtaagac ctatagcagt
     4081 tatgtcaaat cccgtaaccg acttacatga ggatatggca gctgaacaaa aagcacttgc
     4141 aacttattat caacttataa acctaacaga tgacgttgat gttatagatg tattaaaatt
     4201 cttaggtcaa agagaaataa ttcactatca aagatttggt gaagctttaa tggatgctta
     4261 cgagttagaa gaatctcaaa aaatgttcta aaatttttat ttgtataact attattattt
     4321 gataatttat aatttactgt tgtgttaaaa tttataaaaa atataaatat aatttatttt
     4381 ctataaacag agaaaataaa taaaaagcag gtatcaatca aatagtaaca tgtattatac
     4441 tatacattac tatcaatatt gtacctgctt tcatattata agcaataaag aaaaatctat
     4501 ttattaggtt ttataataaa acaattataa aactcttctt gcatttacat atctacttga
     4561 atagtagcta ctatttatat tagatattgt tacttttttt gatgaacctg aagatgcatg
     4621 tatcatatct ccatttccta tgtatattcc aacatgactt acagaaccat tatttgaacc
     4681 ttgtgtatca aagaatacta aatctccact tctaaggtct cctctattta catatgttcc
     4741 atacttactt tggtctctag atactcttgg tatactaact cctgcagatt ttttcataac
     4801 gtactgtgtg aatccagaac agtcaaaact atttggacct tctgctcccc atacatatgg
     4861 ttttccaagt agtgtctttg caaaacttat tacactatca gctccttgac ttggagaatt
     4921 agtcgaataa gatggaattg aagtatcaac agaacctttt tcagcaaggt attgacttga
     4981 cacatatcca gtttgattgc cataacttac ttttgtccat cctccacttt cagaaatact
     5041 tccaacctca gtaccataac ttaatgtagc tattttagaa tgacttgtac ctggtcctgt
     5101 tctaacgttc aatgactttg ctgttacaac ctttgtaccc tttacaaggt catttgaact
     5161 gttttcaaca ttttcatttg tagagtcatt tacactagtt cctaaatatt tacttgaaac
     5221 ataacctact ctaccattag aacttatttt tgaccatcca tttgactcag atatgtatcc
     5281 gacttcagtc ccatatccta gtgttgatat tttagaacta cttgtacttg gtcctgttct
     5341 aaaatttaat ccttttgctg ttacaacttt tgtcgactta actgaagtat cactactttc
     5401 attgctactt ccaaggctat ttattgctac ataatttcca tgcacataac ctgtgtttcc
     5461 attatattta acttttaccc aagaaccttc ttttcctacg tactcaactt tatcaccttt
     5521 gtaaagtttc cccattgatt catggtttgt acctggtcct tttctaaaat ttaccgcatt
     5581 ccctgttatt gttctgcttt ctatactcac actctctact tcagactcat tagcatatgc
     5641 taaattcccc atagtaggca ttattatcgc agttgccatt atactggctg ctattctctt
     5701 ttgattaaaa ttcataactt tccactcatc tcctcttttc tactatcttt atttcacttc
     5761 aaaatataaa agaaataatt gacactatta aaatatcaat tatttctttt atattaatat
     5821 tattacaaat ttgtaactat ttgtctatag taatatttgc ttaccataaa ttaccatgta
     5881 taaattttaa tcttcaatag cctgtaattt agtaatatag ccatctttta ccttttttaa
     5941 aaatggctat attttttaac ttttttgtaa ctttttaaaa tccagttgag ccaaacccac
     6001 cttttcctct ttcggagtca cttaattctt ttacttcttc tatgttaata tcatagatag
     6061 gttttactat catttgagca attttcatat gcttttcaac cttaaaatca tttttcccat
     6121 gatttattaa tataatttta agctctcctc tgtatccttc gtcaatagtt cctggtgtat
     6181 ttaatacagt aacagaatgc tttaaggcaa gcccacttct aggtcttacc tgcgcttctg
     6241 tcatagtagg aagctctata catatcccag ttttgataag tttagtctct ccaggaggta
     6301 ttacaacctc ttctatagaa tacaaatcca taccagcatc acctttgtga gcaaaattag
     6361 gtattatagc atcatcattt aattttttta cttttaaatt gtacatctta tatctccttt
     6421 caactttcta taaatattat aacttaaatt tcgacctctt gaagtttttt atttttttgt
     6481 ggtaatataa ttattagtgt tttaaaggag gattttttat gaaacttgta cttgctgaga
     6541 aaccatctgt ggcaaaaact atagcatcat ttttaggtgc aaagaccaga caagatggtt
     6601 actttgaagg aaatgattat ataattactt atgcagttgg acacttagta tcattatatg
     6661 atatgaagga ttatgataaa gataaatact ctggctcttg gaaaatgaac aatttcccat
     6721 ttgttcctga ggataagttt aaatttaaga ttgatagttc taaaactaaa caattcaaca
     6781 ctgtaaaaaa gcttttgaat agaaaagatg tagaatatgt aataaatgcc actgacaatg
     6841 accgagaagg agaacttata gcatttttaa tatttttact ggcaaaaaat aaaaaaccag
     6901 taaaaagaat cttggtaaat gaatggacac cagaagatat tacaagaggt ataaaaaacc
     6961 taaaagatga atatgaaatg agaaatcttc aagctgctgg atatactagg cttattactg
     7021 actggttgat tggaataaat ttcacttctg ttgctacatt gaaatatgga aatgggaaac
     7081 tattaaacat aggtagggta attcttccaa cagttaaatt agtctatgat agagatatgg
     7141 aaatactaaa ctttgttcct aaaacttatt atgaaataga aggacatttt aaagctgaag
     7201 ctggtgaata taaaggtaaa tatgttaagg gtaaagaaag taaatttgat acacttgaag
     7261 atgcaaataa aattatagct tcaattacat ctgaaactgg taaaatacta gataaaaaag
     7321 ttactatgtc aaaggaatat gcaccaaaac tttttagttt gacatcactt caaggatata
     7381 taacttctaa gtattccaat tttacatctg ataaggtttt aaatgtctgt caatctctct
     7441 atgagggtaa tggtaagggt ggatacataa cttaccctag aactgattcc attttcttag
     7501 aagaaagttt ggcttcaaaa gcttctcaaa ctttagataa attaaaggtt ggtcttgaat
     7561 atgaaaataa aattaaattt tctaaaacta aaagagtttt tgattcttct aaagtagact
     7621 cccatagtgc tataattcca acttatataa ttccaaaaag tttatctaag gatgaacaat
     7681 tagtttatga tgccataaaa gatagatttg tagcaaactt tatgcctcct gctgaatatg
     7741 agaatacaga aattaagact gaagtagata attgtacttt cttaacaaaa ggtaaagtgc
     7801 ttaaatcaaa aggatacctt gaggtatata ataaggaaga aaaaaacgat ttacttccac
     7861 ttgtaaataa agatgatgtt gtagatgtgt tagaaataaa accattaact aagcaaacta
     7921 ctccacctaa accatatact gaggacaccc ttttaaaggc tatgaaaaat tgtggtaaaa
     7981 atgtgcctga agaagataca acagtgctat ctggatattc gattggaaca tcagccacac
     8041 gtgctgatgt gttaaagaag ataagtcaag tgggctatgt aaagaaaaaa gggaaatctt
     8101 attctataac agaccttggg aaaaatttag ttgaaatttt tcctgttaaa gacttatttg
     8161 atgtagatta tactggaaaa cttgaaaaaa gtttaagcga tattcaaaaa ggaaagtata
     8221 caagaaaaga atatcttact aatatcatta gttttatata tcaaaatgtc aatattataa
     8281 aacaagatgc ttcaaaaaat ataaacacag aagcatatac tttcgattca aaaactaaaa
     8341 aatttactaa aacaaaagaa aaaaaacaga aaaaagatgt aactaatact gaaaaagaag
     8401 ttgctatatc aaaagattct aataacaaaa aagaagttaa aaatggtgat gaaaatcaat
     8461 ctcttggcaa atgccccatt tgtcaaggtg atgtactaga atttgacaaa ggctttgctt
     8521 gtaaaaatca taaagaatgt aaatttgtta tttggaaaaa tgataagtat ttaagtcttt
     8581 ataaaaagaa agttaacaaa acaatggtca aaaatatttt aaaaaaaggt gaaactaaag
     8641 taaaatcttt aactgctaaa aacggtaata aatttgatgc tacattaaaa tatgataaaa
     8701 atccagatac cggctatttc aattggaaaa tagagtttga caactaaata ttcataagaa
     8761 aagaaatagc agtagtataa taatctatat tatattactg ctatttcttt tcttatgaag
     8821 ttttaacttt tttaatagtg acttttacaa tcacactcta acctaatttc tttactattt
     8881 ctgataactt taatgtatgt tcatctaata aagttagttc ctcttcagaa agattagata
     8941 atttttttac tagagattct ttgagaatag cttttactgc atcaaataat ttaaatgctt
     9001 tatctgttaa ttcaacccta agaatccttc tatcttttgg gtcaggaaat ttattgacta
     9061 aaccatgttc cactaatctg tcaataatcg gagtcatatt tgattttaaa attcctaaat
     9121 tagaagctat ttgagaaatt gatgatgaat ttgatgtcat aagataaaga attaccttag
     9181 catgtgatgg agccattgga cattcttcaa aataagcttt cattatatca gagttatttt
     9241 gaaacttttt taacaaatcg ctttctttaa atatattatt ttcaagcaca aataaaaaat
     9301 ctaaaaaaga gtccgctatt ttaagtatat tgttgttatc catatttgta acatcccttc
     9361 aatagataat ataataaaac attgttgttt aatatagtat aaattataaa ctattaataa
     9421 atttaaaaca agaaaaagtt attgactcat aataattata aatttataat caaattagag
     9481 aattataaat tgccaataaa gggaagtttt ttaattagtc taaaactaaa cttaaattat
     9541 aagacacttt aaattctaca acaagctagt gtgcaatata taataggagg agatatgaaa
     9601 agatatttaa tagttttctt aatgtgtagt tcactgtttt tagttggatg tggaaaaaca
     9661 aaagaatctg aacctgagaa accaatagca gtttctgttc aaaaagcagt aggtggagaa
     9721 attgaaaaca caaattcttt ctcaggaact acaaaagtta aagatgaaac agctgttact
     9781 gcacagactg ttggcacagt tcaagaagtg tatgtaaaac ttgggcaaaa tgtaagaaaa
     9841 ggagatgaat tattgagtat aagcagtcca gagctagaaa atagtgtaaa gcaaagtaaa
     9901 gcctcactag atttagcaaa agcaagttat tcaagtgcca ctggtggttc attagaggca
     9961 caagtaaatc aggcaaaaac agctcttgac aatgcaaaga ttcaatatga ggagtctcaa
    10021 agaaattatg acaataataa gattctttat gaacaggaag tcattagttt agaccaattt
    10081 aaaaaaatag agttttcttt agagcaaaca aaacaacagt tagattctgc tcaaagagca
    10141 tacgatacag caacttcaaa aagtatacct caagctaaag cacttgctaa aaaacagctt
    10201 gaccaagctc aagtatcata taatttagca atgagtaatt tagacaaatt aacattaaca
    10261 tctccagtag atggtacaat aacagctaaa aactttgatt caaaggaaat gataacacaa
    10321 tcacaaccag cttttattat ttctaatcct aatatactag aagttgattt aaatgtagca
    10381 gaatcagata taggtaaatt taagaaagat ggaaatgtag atgttataat tgaagaccaa
    10441 aggattttag gaaaaataga ttatgtacct tcagtagttg accctcagac atctttatat
    10501 cctgttaaag tacttgtaaa taatgcaaac aacaaattta aggcaggtat gtctgcacaa
    10561 gtaaacttaa gtattgaaaa agaaaatggt gctgttacag tgcctaaaaa agcaatattt
    10621 gaagaaaatg gtaaaaaata tgtatacata gcaacaaaag acaatatagc aaagaaacac
    10681 ttggttcaaa ctggaattgt aacagaggat aaaatagaaa ttaaaagtgg tgtaagtgat
    10741 aaagatacag tgattattgg aggaataagt cttatttctg acggaactaa gatatttcct
    10801 gtagaaaagg agaagtagac tatgaattta acacagactt ccgttaaacg tcctctcaca
    10861 atcataatgg tctttttagt attaatagtt tttggtggta ttggatataa aaaaatgtca
    10921 ataaatctaa tgcctgatat agaaatacca gttgtaatgg tcatgactac ttggacaggt
    10981 gctggtcctc aagacgttga tgaacaagtt agtcaaaaag ttgatgaaag tttatctgcc
    11041 gtttcaaatg taaaatctac tatatcaagt tcacaagaaa gtgtttctat ggtagttgct
    11101 cagtttgaat ttggaactaa tttggatgaa ataatgaatg atgttaggtc taaagttgat
    11161 gcattacaga ccagcctccc agatgatgca gcaaaaccaa cagttttaaa gctggatatg
    11221 aatgcacagg ctataggtca acttgtcata tctggaggaa atgaaaatag ttcacaagcc
    11281 ttaagaaaat acgctgaaga tgtaattcag cctaaaatag aatccataga tggtgttact
    11341 tcagcagacc ttaaaggtgg agaaaaagca caggttaatg taatagctga cccagctgtt
    11401 ctttcaaatt atggagttag tttatccact ataaaaggtg tcttatcctc tagtaataaa
    11461 acattccctt atggttcaat tactcaaggt gaggataaaa tagttttaag agcaattgat
    11521 aaattagaat ccctagatga tataaaacag attcaaattc ctgtaaaagg aggaaataca
    11581 gttagattag ataatctttg tagtgtagat tatggatatg cagacaaaga tagtatttat
    11641 agatacaatg gaaaagacag tttggtaata gatatttcta aacaacaaga tgccaacact
    11701 gtaaaagtta tgcagagtgc atacaaatac gtagatgaat tgaataagga aaatcctcaa
    11761 tttaaaatta aaattgctaa tgatacaagt tcatacataa gtgaatcaat aaacagtgta
    11821 atgagcaact tgcttataag tgctgttatc gcatttattg taatatttgc tttcctaaaa
    11881 agtgtaagag cttcactagt tgttgcagtt gcaataccaa tttctataat aggtgctata
    11941 gcaattctct actttactgg tgaaagttta aatcttatta ctgcaagttc attggtaatt
    12001 tcagttggta tggttgttga taatgctaca gttgtaattg aaaatatatt taaatatagg
    12061 aaaagtggca gattgagtct tgatgattgt gctattgagg gaacaagaac tgtaacaaat
    12121 gcaatacttg catcaacatt gaccacgata gcaatattct tacctatcat gtttacagaa
    12181 ggtatagcta aaataacttt tggggcatta ggtaaaacac taatagcttc actagcattc
    12241 tcctttatag gtgcaattac actcgttcct agtgtatttg caaaattaag cagaggaaaa
    12301 aatagtcaaa agatgcaaga aaaagatagt cctatatttg ataaggtaag tgaagcatat
    12361 aaaaaattat taagtgtatg tttaagacat caacgcttag ttgtaatatc aagtttactt
    12421 atttttgtag cttccttatt tggaatggga tttataggta tggactttat gcctgcagca
    12481 gacaagggtg aattatctat ttctatagaa cttccaaaag gtcttagcct agaatccaat
    12541 gattactatg tatctatgac tgagaaaaaa gtctcagata ttccagaaat taaaacttta
    12601 ataacaactt tgacatcaga ttcaaataat agtaaagcaa gtatagcaat tgaattagaa
    12661 tctcaaaaaa atagaaaaag aactactgat gaaatagaaa aagagataat tgatagagtg
    12721 gctactgtac cagattgtaa aataaatgta agtcaaaaca gtagtgttat gggtggagct
    12781 ggaggagcag acttcacatt agaaatgaaa ggtcctgatt tagatgtatt aaaaatactg
    12841 ggaaaagaag ctgaaaaaga atttagctct ataaaaggat ttagaaatgt tgaaacttct
    12901 cttacagata caacacaaga agctcaattt actatagata aacataaggc tcaagaatat
    12961 ggtgtaaata ctgctgaaat tgcaggcatg ttacgtacag caataaatgg tgactccgta
    13021 accactgcta ctattgatga ttacaaagtg gatgtaaacc taaaatttaa agcagatact
    13081 attgatagct tagaagatat aaagcaacta aaagtaaact ctgcaactgg aaaagaagtt
    13141 cctcttggtg ctttttcaga tataaaaatg gcagatggtc tacaatctat atctaggact
    13201 gatggagatt tttctgtaaa tataactgct acacttgaca atttagatgc tggtactgct
    13261 acaaaactag cactagaaaa aacgaataaa atggtaattc ctaaagatta ctctataggt
    13321 gttggtggtg aaacagaaat gatgaatgaa tctatggcaa gtcttggatt ttcaatggtc
    13381 atagctatag cattagttta tatggtaatg gtagctcagt ttgaatcatt tagtaaacca
    13441 tttataatca tgttctgtgt tccatttgca ttcgttggag ttgtgctttc gcttctagtt
    13501 agtggtcaaa gtttaagtac tgtcggtatg ctaggtataa tcttacttat cggtattgtt
    13561 gttaataatg gtatagttct tattgactac atagagcaac ttagaaaaac aaatcgtaac
    13621 aatgatttaa ttggtttggt ttcaattggt tgtgcagcaa gattaagacc agtcttaatg
    13681 actacagcaa caacaatttt aggtatgttg ccaacagcac tatctcttgg tgaaggtgga
    13741 gaaactatgc aaccactagc catagttata atgggtggtc taactgtatc tacacttata
    13801 actttggtgc ttatacctac tatatactta atatttgata aatgggaaaa taagttttca
    13861 gacaaaatgc ctaaaatatt tggtaagata acagataaaa taggaagtat aaaacgtaaa
    13921 ataataaaga aaccaaaatt gaaagctaaa gtttaaatag aatggagggt gattgtattg
    13981 aataaaaaaa tgagcaaaat agttgcaatt ggctgtggtg ttggtataat aactgccaat
    14041 ttaatcccag tttacgcaga taataatact tcttctataa acaacgttga taattctatt
    14101 ttaagtgact caaataaaag aacatctgaa aaggaaattt tgactgctag agaagctgtt
    14161 agtgctgcac ttaaaaatag tgaaaaatta aaaatgaaat cagaagaaat aaaaatgtta
    14221 aaagaaaagt tagaagtaca agatgagttt gatagtttta ctggaagtga caactcgttt
    14281 ccatacgacc aaattggact tcttaaaaat caaagtgaac aggctaaagg ctttatggag
    14341 gaccaaatag caaatgatat tactaataaa tttaacgatt tagtttcaag agaaaatgag
    14401 ctagataaga taaaaaacaa tcttgaaata aaaactaaag aaattaaaga tatgaaatta
    14461 aaaaaagatt tagggttagt aacatcttta gaaactgaat cagctgaact tgaacttcaa
    14521 acactacaaa atactcaaaa agctaaactt caagaactaa aaaacaatca agactatttt
    14581 aagttattga ctaatataga tttaaataat tatcaattgg ataaagaata tagatttgaa
    14641 tcttttagag taggtggctc agttgattca tatatggaag gtaaggttaa tgaatattta
    14701 aaatacgacc aattgatttt agaacgtaca gaagaaagct ttaatgataa agatgagaac
    14761 aaagcagatt taccagatag acctaatctt acaagaccag ttgctcctac aaaaccagag
    14821 caaggtaacc taactgatga tgaatacaag gttcttatgg ataagtatga aaaagactac
    14881 aagagatatg aaaaagaagt tgaagcatac aatcttgaaa gacagactta tgctgctggc
    14941 cttactactt atgcaaatta tcttcaacaa aaatttaata ccgaaaatgg tctagtaaca
    15001 ttagaagaca gtaaaaaagc tcttaaaaaa gggttaattg atagctatgc tcaattactt
    15061 gctttagaag atacaattca gattactaaa aaacaacttg atttatcaga aaaacaatta
    15121 aaaaatacta aattaagata tgatttagga ttaattactc taactgatta taaaaaacaa
    15181 gtggtaagta atcaagatgc taaaaatagc tatgacactc ttattgtgaa ttataactca
    15241 ctaaaaaatg gtatagaaaa accttggata cttaatactg gaaaataatt ctggtaaata
    15301 gaaagattac tttaacatac gtttgattaa aaaatagtta tgcacactcg tggctttagt
    15361 catggaagat tcagaaggca aataatagaa atagattagt aaaaatatat ttacttataa
    15421 aactgtatac ttttatagag gctatctcaa aattggatat gtccctgtca agtagacaga
    15481 cttaaaaaag gctatcttta tgcggaatga gttcgatatt caatcgaact cattcc
//
LOCUS       DACTGW010000075        15487 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.075, whole genome shotgun sequence.
ACCESSION   DACTGW010000075 DACTGW010000000
VERSION     DACTGW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15487
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.075"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            134..682
                     /locus_tag="I9573_001856"
     CDS             134..682
                     /locus_tag="I9573_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT4796479.1"
                     /translation="MGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKADIEPVL
                     LKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEERLSEFI
                     NGNTLKYDFVKMPHHGRYDKLTETFLESTSPQYAVITCSEKKEPEEGVLKILERLNIK
                     TFLTSSGEVVINSDGKTLSVNQ"
     gene            801..992
                     /locus_tag="I9573_001857"
     CDS             801..992
                     /locus_tag="I9573_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796480.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            1215..1565
                     /locus_tag="I9573_001858"
     CDS             1215..1565
                     /locus_tag="I9573_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796481.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKTVTSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            1827..3332
                     /locus_tag="I9573_001859"
     CDS             1827..3332
                     /locus_tag="I9573_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAT4796482.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            3722..4819
                     /gene="dinB"
                     /locus_tag="I9573_001860"
     CDS             3722..4819
                     /gene="dinB"
                     /locus_tag="I9573_001860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAT4796483.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGKPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            5075..6211
                     /locus_tag="I9573_001861"
     CDS             5075..6211
                     /locus_tag="I9573_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAT4796484.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            6204..7751
                     /locus_tag="I9573_001862"
     CDS             6204..7751
                     /locus_tag="I9573_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAT4796485.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            8085..8951
                     /locus_tag="I9573_001863"
     CDS             8085..8951
                     /locus_tag="I9573_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAT4796486.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            9092..10234
                     /locus_tag="I9573_001864"
     CDS             9092..10234
                     /locus_tag="I9573_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAT4796487.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            10618..11856
                     /locus_tag="I9573_001865"
     CDS             10618..11856
                     /locus_tag="I9573_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796488.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYNL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            11885..12772
                     /locus_tag="I9573_001866"
     CDS             11885..12772
                     /locus_tag="I9573_001866"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HAT4796489.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYSLYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            12806..13435
                     /locus_tag="I9573_001867"
     CDS             12806..13435
                     /locus_tag="I9573_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HAT4796490.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADKVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            13467..13904
                     /locus_tag="I9573_001868"
     CDS             13467..13904
                     /locus_tag="I9573_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HAT4796491.1"
                     /translation="MGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVYVFSVIGSTL
                     VSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGIILFVGIPLPT
                     TGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            14154..>15487
                     /locus_tag="I9573_001869"
     CDS             14154..>15487
                     /locus_tag="I9573_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT4796492.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIRYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYK"
BASE COUNT         5877 a         1629 c         2680 g         5301 t
ORIGIN      
        1 gaaccgtagg aactacgggg atagcctgtg gagaattagt aagacatatg ttagtatgca
       61 aaattctatg aagcaggaac cctgtgactt tagtcatagg aggttcagtt taacccattt
      121 agataaggac catataggtg gagtagatag tgtgttatct agtgttaaag taaagaatat
      181 tatacaagca aattataaga aagatagtaa gcaatatgat gaatatattg actcacttaa
      241 aaaagctgat atagaaccag tgctactaaa agataatatg aatattgtaa tcaatagtgc
      301 agaaataaac atacatccgg cttcaaaaag caagtacgag agttcaaatg attattcaat
      361 aatcactaat ataagctatg gagcacataa gtttttattt gcaggagatg cagaggaaga
      421 aagattgtca gagtttataa atggaaatac tttaaaatat gattttgtta aaatgcctca
      481 tcatggaaga tatgacaaat taactgaaac atttttagag tctacttcac cccaatatgc
      541 tgttattaca tgttcagaga aaaaagaacc agaggaaggt gttttaaaaa tattggaaag
      601 attgaatata aaaacatttt tgacttctag tggagaggta gttatcaata gtgatggtaa
      661 gacattgagt gtgaatcaat aatttttttt aaaatatcta aaaaaaattt aaaaaaatca
      721 ttagcaaata ttacccctta aacatagtat attttaggag ctgaaactcc acatacaaaa
      781 tgactaggag gtaacatatt atgttggata gattttttgg tgaaggcgga tttggtggat
      841 gtgcttggtg gataataata ttattcttct tattcttagc attccaagat tgttgggctg
      901 aaatggactt atgtgcatgg atacctttct taatattatt attaatactt tgtgaatgtg
      961 gtgacgtttt tgatggagga tgtggatgct aaaaaaatca atttagatga tttaataaag
     1021 cttatggtgg gtataaataa aataccaaac caatcacaat ctagtgtttg aaggtagtcg
     1081 gaaatccccc gctaccttct tttttttatt gttagaattt atttttgaat tatttcattt
     1141 acatataata atttttacaa taaagtctga tataattaaa aagaaaataa aatttattta
     1201 taggagatgt aaaaatggct atagtggata agattttcaa tgagataaaa cttatggttg
     1261 caagactttt attgggaaaa aaatacagtg aatatgaaga aaaagattta gtgtataata
     1321 cagaagaaga agttatactt ataactttaa aaagattagt ttttcaggga aatgtaaacg
     1381 aagcagaaga aattttattt gataaagcta agactgtaac tagtgaaaat atgcagtata
     1441 tagcaataga attttatact atgcttatgg aaaagactga tgaagaatta gaagctatga
     1501 atttttctaa acaagaagtt tatcaaggta tagaagatat tagaaaagta ttgaatttag
     1561 aataaaagat atatgaaaat attgaaataa attaatgtta aggtgagaat attatactta
     1621 tatataaaaa tataatattc ttattttatt agttatatta ggtatatctt atttaccata
     1681 aaactatctt gaaaaaaatt aatcttaatt gtataatatt actttgagtg aaaaagtatt
     1741 aacatttttt tataagatgt tttactaaaa aaatatatat aaatatactg ttatcaacaa
     1801 gaaatttaat ttagtgaggt gttaagatga aagtaggatt tgaccacaaa aaatatttag
     1861 aagagcagtc taaatacatc ttagaaagag taaataattt tgacaaactt tatttagagt
     1921 ttggaggaaa attgatggga gaccttcatg caaaaagagt acttccagga tttgatgaaa
     1981 atgcaaagat aaaagtatta caacatatga aagaaaaagt tgaagttgta atttgtgtct
     2041 atgcaggaga tatagaaaga aacaaaataa gaggagactt tggtatcact tatgatatgg
     2101 aagttttaag attaatagat gatttaagag gttatgaact agatgtaaat agtgtagtaa
     2161 ttactagatt tgaaggacaa cctgctacaa ctgtatttat aaataaatta gaacgtagag
     2221 gaattaaagt ttataagcat tatccaacaa agggatatcc gtcagatgtt gagactatag
     2281 ttagtgatga gggatatgga gcaaatgaat atatagaaac tacaaagcca atagtagtag
     2341 tcacagcacc aggacctaat agtggaaaac ttggtacttg tctaagtcag ttgtatcatg
     2401 aaaataaaag aggtaatgta gttggatatt ctaagtttga gacattccca gtttggaatg
     2461 tacctgtaaa acatcctttg aatatagctt atgaagcagc tacagttgat ttaaaagatg
     2521 tgaatatgat agattcattc caccttgaaa cgtatggtga aatgtcagta aactacaata
     2581 gagatttgga attatttcca gtgcttaaaa aaataataga aaagataact ggaaaagaat
     2641 caattttcca atctccaact gatatgggtg ttaatagagt tggctttgga ataatagatg
     2701 atgaagtaat aagaaaagct tctatagaag aaataataag aagatacttt aaaactgcct
     2761 gtgagtataa aaagggacaa gttgataaag gcgcttatga tagaatgaaa atgattatgg
     2821 aagagcttaa cttaaagcca gaagatagaa atgttgtatt acctgctaga aattacagta
     2881 ataaattaaa agaaagtgcc gacaagaatg atacttgtcc tgtagttgca ttagaattag
     2941 aagatggtac aatattaact ggtaagggtt ctgatttaat gaatggaaca gcagcagtag
     3001 tattaaatgc cattaaacat ttagccaata tatctgatga tatgcactta atatcacctg
     3061 taatattaga acctatcata aacttaaaaa caaagacttt agctagtaga aatgtttcac
     3121 ttagttgcca agaggtgtta actgcactta gtatttgtgc tgtaactaat ccaactgcac
     3181 agtttgctat ggaaaagtta gcattattaa aagggtgtca agctcattct actactatat
     3241 taaatagaga tgatgaacaa acatttagaa agcttggtat agatgtaact tgtgacccag
     3301 aatatccttc acaaaatctt tactatagtt aaaattatat aaaaaaatat gggctgtttt
     3361 aaagtaatag tttgagcagt ccattacaat tataataaaa tcagtaggct gtcttgtata
     3421 agacagtctt ttgtttttaa aacatcccat aaattaaata tatatactgt tatccataag
     3481 gaattttaaa tttagttcct tggaatacct attgtaaagc ttttatatag tttttgcaag
     3541 tgtttttaag gattttttat aaattatatt gaagttaatg gattatagat ggttttattt
     3601 atggaaaata tgaatatata aagaaaataa tgaaaaaata ccaaaaagaa acataagttt
     3661 ggtataatta aaaagtaata taaataaaat taatctattt atactttaaa aggtgataat
     3721 aatgaaacat tatagaaaaa taattcatat agatatggac gctttttatg catctataga
     3781 acaaagagat aataaaaaat taaaaggaaa acctgttatt gtcggtggaa atccacaaag
     3841 ccgaggtgtg gttgcaacgt gttcttatga agctagaaaa tttggtatcc attcagctat
     3901 gccatcagct gtagcttaca ataggtgtcc atatgcagtt tttgtaagac ctagatttga
     3961 tgtttataag agtgtgtcag agcaaataag agatatattt tatagataca cggatttagt
     4021 tgaaccatta tctttagatg aagcttactt agatgtaaca aaaaataaaa aaaatattga
     4081 ttcatcaatt gagatagcaa agcaaataaa aaaagatata tttagagaag ttggtcttac
     4141 ttcttcagct ggtgtgtcat acaataagtt tcttgctaag attgcatcag acttaagaaa
     4201 gccaaatggg ttaactgtaa taaccgaaga aaatgcccag gactttttag ataaattacc
     4261 tgtgaataaa ttttttggtg ttggaaaagt tacttcaaat actcttaaaa atttaggtat
     4321 aaaaactgga tatgatttaa ggtgtttaaa tttatttgaa cttgaaaata tattcaaaaa
     4381 aagaggttat gaattatata aatttgctag aggaatagac gatagaccag tggaaccaaa
     4441 tagggttaga aaatctgttg gggctgagac tacattaagt cataatttgg atattgatga
     4501 agaagaaaca cgcaacattc ttgatgaact ttgtgaagaa gtatgtcata gattaaaaag
     4561 ctctgagaag tttggaaaaa cattaacatt aaaaattaaa tatgaggact ttacaaagat
     4621 aactagaagc cttagtttag aacactatat tgacgaatat aatgatataa gaagtggtgt
     4681 agataatttg ttgagaaatg ttgaggttaa tggaaaacaa attaggctat tgggagttac
     4741 aatatcaaac ttaagtgata aaaaagaaac ttataaagat attacattat ttgaatacat
     4801 ggatagtatt caaatgtgag ttttgtttaa atgaaataac actagcttta atactatata
     4861 attgaggcta gtgttatttt atgaatattt ttatactttt tgttttttga cattatatac
     4921 tatatataaa tactttatat ttgtagcaat attcagacaa atagtgacct aaaaatcgta
     4981 ctttgtttac ataaataaaa tagtatgata taattataaa aacaacttat aatattggag
     5041 aaggttgctt gttcgaaaaa ggggatgata aattatgata gagataagaa atgtaacaaa
     5101 aaaaatagga aataatgtta tattagatga tatttctcta gttgtagaaa caggtacatt
     5161 ggtagttttg attggttcaa gtggttgtgg aaagactact actttgaagc ttataaacaa
     5221 attaataaaa cctacatctg gtgagatata tattaatggc aaaccaattt ctcaggaaaa
     5281 tgaaatagag cttagaagaa aaataggata tgttatacag aacacaggcc tattccctca
     5341 tcttacaata aaagaaaata tagagctaat tccacgccta aaaaaggaaa aatccgttga
     5401 agaaattgaa aaaagaacgt tgcaactact tgagatggta ggacttgata gtgatgaatt
     5461 tttgaataag tatccttcag aacttagtgg tggacagcaa caaagaattg gtgttgcaag
     5521 agccattgct actgatgctg aaataatatt gatggatgag ccttttagtg ctttagaccc
     5581 aataacgaga acatctttac aagaacaatt attttctctt caagatgaat tgaaaaaaac
     5641 tataatattt gtaacgcatg atatggatga ggctttaaag atagctgata aaatttgtat
     5701 tatgaaggat ggaagaatag cacaatatga tactcccgaa aatatactta gaaagcctgc
     5761 aaatgacttt gtaaaagatt ttatcggaga agatagagtt tgggataatc cagaatatat
     5821 aaaagcaaga gatattatga taaaaaatcc tattgctgtt aattccacta gaactgtaac
     5881 tcaaggaata gaaattatga gaacgagtaa ggttgatagc ttactaatta tagatagagc
     5941 aaaaacatta aaaggtattg ttactgtaaa agatatgaaa gatattgatg ataagtcaat
     6001 attacttgct gatattatgt cgagtgagcc tttgcatgta aatgaaggtg ataatttagt
     6061 agaaatacta aatgtaatga atcgtaattc ggttggatat attccggtta tttctgatga
     6121 aaataaactg gtagggctta ttactagaag tagtttactt tctgttttaa gtgaacaatt
     6181 cttagaaatg gaggtgagtg ttcttggcta atttttttaa ctttatacta ttacaaaaag
     6241 ataagattat agagctttta atacaacata tgagtctaac tgttacatct attttaatag
     6301 ctataatagt tggtgttcct cttggaataa ttatttcaag aatctcatct cttagaaagt
     6361 ttgtacttgg atttgtaaac ttagtccaag cagttccgtc tatggcactt ttgggtcttc
     6421 ttgtacctat acttggcata ggaagtaaac cagccatatt tatggtagtg gtttattcat
     6481 tactaccaat tgtaaaaaat acgtatatag gtataacaag tattgaccca gtagtattag
     6541 aatcagctaa aggtattggt cttacaagaa atcagacatt atttaaaata caattccctt
     6601 tagcacttcc tataataatg ggtggagtta gaatatctgc agttacagct gttggtttga
     6661 tgactcttgc agcatttata ggtgctggtg gtttaggata tttagtattt tcaggggttc
     6721 aaactgtaaa taataatatg attttagcag gggcaatccc atcatgtata attgcactgt
     6781 tagttgatta tttatttagt aaaattgaag ttgcagttac tccaaaagga ttaaatccaa
     6841 aagcacctaa gaaaaattat atagcactta aagtaatatc tgtagtggtt gttgtttcaa
     6901 tgttgttcgt tgtttttagt tctagttttt caagtaaaaa agatacaata actattggtt
     6961 ctaaagatta tacagaacaa cttatacttg gtaatgttta tgctgaatta gtagaaaaaa
     7021 atacgaatct taaagttaag aagaatttaa atctaggtgg ttcatctgtt gcgtttaatg
     7081 caattaaatc tggtgagtta gacatgtatg ttgactatac aggtacactt ttggtaaatg
     7141 taatgaaaca tgctccgatt aaagatgctg atgaagcata taatgttgta aaagatacta
     7201 tggaaaaaga aaatcagtta actttacttg accctcttgg atttaataat acttacacat
     7261 tagctatgat gcctgaaact gctgaaaagt atggaataaa tactatttct gatttgacta
     7321 aatatggtaa agaatttaca ttctcaccaa ctttggagtt tgaaaataga gaagatggtc
     7381 ttgtagggct ttctagagat tatggtttaa aattcaaaga tgtaaaagca atgaatggaa
     7441 gtttaagata tacagcttta gataataatg aatctcaggt tatagatgca tttttaactg
     7501 atggtcttat taaaaagttt aatcttaaga tattagagga cgataaaaat ttctttgtac
     7561 catattatgc agcaccatta gtaagagaag atactttgaa gaaatatcct gagttagaaa
     7621 aagttatgaa tatgctttca ggaaaaataa atgaagaaac tatgagagaa cttaactatc
     7681 aagtggatga acttggtaaa tcaccagaag aagttgctca ttcattctta gttaaagaag
     7741 gcttagtata aaaaattaat agtttatatc aaaaatttaa gggagtgtct caaaatgaac
     7801 tttttagttc atgaggcact cttttttata tgaaatcaaa tatattttat catttcaaca
     7861 ataaaaaaga agcttatctt tattttgaga cagtctctta tttttatgta taccaaaacc
     7921 atacctacga tttttattta taattaagtt tcaaatagaa tgaacaagta caaccaattt
     7981 tgaatataaa tatagtaaat gtgcttatat acgattttat tatctgggtg attgatattg
     8041 atagatagct ttctttattg aatcgaaggg agggggaatt tgttatggat aactataaga
     8101 aagtgaagca agtattgtgg ataattttat ttgctaattt tgcagtagca ttattaaaaa
     8161 tcattattgg taatcaaata aaaagctata gtatgacagc agacggattt cattctttgt
     8221 cagatggagc atctaatata gtggggctta taggtatttt ctttgcatct aaaccaaaag
     8281 ataaaaatca tccttatgga cataaaaagt ttgaaattat aaccagttta tttataagtg
     8341 gtatgttgtt tgttatagca attaagataa ttcttagtgc agtgctaaga atagctaatc
     8401 cagttgtacc tgctataact atagaaagtt taattgcttt aattattaca ctttttataa
     8461 atatttttgt atgcatgtat gaatatagaa ttgggactaa actaaatagt tatgttttaa
     8521 tttcggattc attgcataca agaagtgata tatttgtgtc tctaggtgta ttggttactt
     8581 tagttggtgt taagttagga tttccagtaa taatagaatc tatagttcct attattattt
     8641 ctgcattcat aatttattcg gcttatggaa tatttaggcc atctattgga attttggtcg
     8701 atagagtggc tgtagatgag gattatataa aagaaattgt gtttgaattt aatgaggtaa
     8761 gggatgttca caatatcaga agtagaggta gcaaaagttc tatttatatt gacatgcatg
     8821 taatggtaga cccatttata agcgtagagc aatctcatga tttaacacat aaaattgaga
     8881 aacaaataca agaagaaatt aatgaaaatg ctcaagtaat agtccatatt gaaccttttt
     8941 atagttttta acttagctat aaaatacaag ttataaaaaa atataattta taaaaaaaat
     9001 attataccat aaataattta taaaaattat ttatggtata atattttcta taaagaaatt
     9061 aagtcttaat atatctaagg aggaatttac aatgagatac gttgaatatg gaaagacagg
     9121 gaaaatggtt tcagtagttg gctatggagg attaaggttt gatttagaaa aaagtaatca
     9181 agaaaatgct gatttagtca aatatgcgta tgaaaaaggt attaattact ttgatacagc
     9241 gccagggtat tgtgatgaca gaagtgaaga tatatttgga ctagccttta gacaaatgat
     9301 aaaggaaggt aagaccgatt tttatgtatc aactaaagaa aagccaaaag catgtgatac
     9361 atcagaaaaa gctatagagg ctgtaaaaaa atcaatagaa cgattaggcg tatctaaaat
     9421 aaatttttat catgtttgga atattagaaa gatggaacat tacgaactag caatgagacc
     9481 tggaggtcaa tatgaaggat tattgaaatg taaagaagaa ggattgatag accatatagt
     9541 attttcatct catcaaccag gagatgaggt tatagatatt ctaagtgaaa ataagtttga
     9601 aggtgtaact atgggaataa atatattaaa ctttccatat aggctaaaag gtgccaaata
     9661 tgctgttgat aatggatatg gtgttgtggc aatgaatcca ttaagtggtg gaactattcc
     9721 aaagtataat aaagaacttt cttttctatc aaaagatggc gaaagtacta tagaatcagc
     9781 tttaagattc aacataggaa tttcacaaat aagtatatct ttgatagggt ttaataaaaa
     9841 acaagatata gatgatgcat gtaaaattgc agacgaaaat agaatttatt cagatgagga
     9901 tatacatgat attgagacta gattaaataa gaatatgaac gaaatatgta caggttgtgg
     9961 ttattgtaaa gtatgtccaa aaggtattaa taccccagcc tatatgcttt tttataatga
    10021 aaagcagatg tttaaaaaga gtgatgaaga aatgacaaag ttagtttatg gtttaggaca
    10081 ttggaattac acaatgaata gtaaggcaaa agctaaagaa tgtatatctt gtggaaaatg
    10141 tgaagttgaa tgtactcagc atttacctat aatagataga ttaaaagaaa ttaaaaaatg
    10201 ggaagaagat ggagctaata gtgttaaagt ctaattaatt ttatactata tttgaaattt
    10261 gtatagtatt tagtaatgtt ttaaaatata gttttaatat ttaaggagac agattataat
    10321 tttgtctcct taaattatgc tttaatttgt tatagtttaa ttccataagc aacattgtat
    10381 atttttatat aaagtttttc aaatatttta cattaatatt gcttacaaat tctcatttta
    10441 tacaacacta atctatgtta tataaacaat acttgactga ttataacatc aataaagtaa
    10501 atattgtggt tgtaaataaa gagttattca attacaataa aacaagtaag aacattatga
    10561 agatggctat taaatataag gaataaattg taggtgtcag caaatcctat ataatgaatg
    10621 gatggtgata tattgatgag taaaatatta aaatataaaa acgagatgtt tttgtttata
    10681 ttagttgtaa taacttactt aataataact aagatatttt tttctaagac tactatattt
    10741 tataatttaa acaatactta tgatgtattg ttagatacag atacaggggt tttattcaat
    10801 ttaaatgtat ttgctataag tcaagacaat agtaagcaca tattgttttc agcaattata
    10861 tcaatttttg catatccaat atacctattt tgtacttcaa tagcaaatcc tgggacaaca
    10921 gattttaata gtgcctatgg gtttggacta atttgtctac aaataataac tagtgctatg
    10981 tcaataactt tagtctttaa tcatattaaa aagataaaaa tgcagagatt aactttaatt
    11041 ttacttacta tgataatgat attttctttt ccacagttat ttatgacttt aaatgtagag
    11101 agatttatat atagccaatt ttcattaata tttttcatag ttattgcaaa taagatgaaa
    11161 ggaaaaaatt cttatttagt agaattagca gctattcctc tatttggaat aactattagt
    11221 aatatatatt tgtatttttt taatatgata tttgagttta aattaaaaat aggaaaaatg
    11281 ttaaaacatt taattacatt tatcctaatg gcatatattt gtgtagtttc aacgaagtct
    11341 tatgagagtt ttatgaacct tggaaatgta atacagtatg atacgaaatt tataagtgga
    11401 gaaccaattt tggaaaagat tgcaatgatt atagagagat tattgtattc ggttttttat
    11461 tttcctggag caagtattaa aaaagggtta tttttgcaaa atggagaagt tgctacaata
    11521 ccagtaattc ttacattatt agctttatgt ttttgtgttt tatcagttat tgaaaattca
    11581 gaaaaaagag ttcctaagct atgtatggga ataattatat tcaatctaac tttacatggt
    11641 atagtaggat ataatctagt caattcaagt attatggcta taaattttag ttttgcagtt
    11701 ataattttat tggcttactt tacgaaagcg cttagaaaaa atgaaaagaa tatgtataat
    11761 atatttctat cgctgttatt ggttactata gtaatcagta atgtcaatgg atttatagaa
    11821 attttaaaca tagggataaa atcatatcct gtttaaaaaa taatattgaa agggtagtaa
    11881 gtttatgaaa cagaccaata caagtataag gcttcttact aaaatttcag attatataaa
    11941 gttaatgaga ataaagcaat atataaagaa tttctttgta ttttcagcta taattttttc
    12001 aaacaacata ctaaatataa atttattttt aaatacgttt attgcgtttg tgtgtttttg
    12061 cctaatgtca tcatcagtat atgcactgaa tgatgctata gacatggata aagataaaaa
    12121 acatcctaaa aaatgtaata gaccagtggc aagtggaaga ataagcaaaa aaagtgctaa
    12181 tatactattt gtagtattag ctttaatttc agtctgttta tcaacagttg ttagttttaa
    12241 tctttctata attcttagtc tctatttgat aaacaatata ttatattctc ttaaactaaa
    12301 gaatataata ctattagatg tgttttcaat atctcttggt tttatactta gggtttatgc
    12361 aggatgtgta gccataagtg ttagtttatc aaattggata attttatgta catttttctt
    12421 atctctgtac ttagctcttg gaaaacgtaa aaaagaaata gaaactctaa gagatgatgc
    12481 agttgaacat agaaaaatat tggaagatta tgacatagaa aatctaaatc aaatgatgat
    12541 agttatatta agttctacaa ttgtatgtta ttcattgtat tctacaagta atccagaaaa
    12601 gccacatatg atatttacaa caatatttgt agtttatggt gtacttagat ataattatat
    12661 aattaacact acaaatgaaa ataatcctac tgatattgtc ctaaaagata atgctttaaa
    12721 aataaacgta atcttatgga taattacatg tttaataata ttgatttttt agtgatattt
    12781 ttagtgatag gatggggaaa tacttatgaa aaaaagtgta ttattacttg atgtagatta
    12841 tactgttata aatactgata gcatgataga tttttttatt tactctttaa aaaataaaac
    12901 ctttaaaact attataaagt tgccatacat tatttttatt ctatttatgt atttaattag
    12961 aatgataccc ttgaagaagg caaaagaggc aatcttttat ccaatagtag attttagtga
    13021 agaagattta aaaaagtttt ttgatgattg cataatgaaa aaaattaacg aatctatgaa
    13081 aaaggtcatt tacaaaaata aagaagaaga taatgttata attatgataa ctgcatctcc
    13141 atatgcatat atgaaatatt ttaagtatta tggtcttgca gacaaggtta taggcacgga
    13201 gtttttttat gaaaattcaa gatataaaaa tcaattcatt ggagaaaact gtaagggaat
    13261 tgaaaaagta aaaagaataa aggctgtatt aggtaaactt gggatagaga ttgattatga
    13321 aaattcatat gcatattcag actctaagag tgatttacca atgctttcat tgactaaaaa
    13381 tgcattttta gttagtaaaa aggatggtga ggttatagag caaataaatt cataacttaa
    13441 tgtcacttag aataggaggg aatagtttgg gatatttaca catattaatt ttatctatgg
    13501 ttcctgtaac agaattgagg ggagccattc ctataggtat tgcaatgggc ttaaatccta
    13561 tatgggttta tgtatttagt gtaataggtt ctacattggt atcgattccc ttgatattga
    13621 cattcagaca tgtattacaa tttttaagag ggaagaaact atttaaaggg attgctgatg
    13681 taattgatag aaaaataaac tcaagaatga agaaattaaa aagtgtttcg ataattggta
    13741 taatactatt tgtaggtata ccactgccaa ctacaggaac atggactgca tctgctatag
    13801 cttccatatt aaaaatgaga ataaaagatg cttttatggg agtatttctt ggcaatttgt
    13861 tatcaggggt tatagtttca gctctttctt tacatataat ataaaactat ctaagtaata
    13921 tacttggata gtttttttat gagtacatgt gaaaaatttt taataataaa taaaaaatga
    13981 gataaagtat ttaaattcag ttagttctaa aatgtttact atggcaaaag aaatatagta
    14041 tagtattact tgtagggaaa gtactctaaa tttgtctgct gataaaatga aatttttata
    14101 tagaataaaa ttttagtgat acattttaat tattggattg aggaggtttt aaaatggaaa
    14161 attcaaacac taacttaggc agtgaaagtg tgggtaaatt attatttaaa cttgctacac
    14221 ctgctattat tgcacaaatt gtaaatgttt tatacaacat agtcgatagg atttttattg
    14281 gtagaatgga aaatggagaa gttgctatgg ctggtgttgg agtggccttt ccaataataa
    14341 ttataataac tgcatgtagt tatttaatcg gtatgggagg aggtccatta gctgctataa
    14401 aaatgggtga gcaaaacaat gatgaggcag agaaaataat gagtaacagt ttttctgtac
    14461 tggtgatttt agcaatactt ttgactatag gttttaagat tggaaaagag cctttacttt
    14521 ggatgtttgg agcaagtgaa tctacaatta gatattcaat ggactattta aatatttatt
    14581 taataggtac agtgtttgtt cagatttcaa tgggtatgaa tacttttata aatacccaag
    14641 ggtttgctac aactggaatg atgacagttg ctataggtgc tttaataaac ataatacttg
    14701 acccgatttt tatatttggc tttaatatgg gtgtaaaagg agctgcatta gctactataa
    14761 ttgcacaagg cgtttctgct atttgggtac ttatgtttct ctttggaaag aaaagtatac
    14821 ttaaaataaa aaagaagtat atgataccaa aagcaagtat catcttacca gtgcttggct
    14881 tgggaatctc tccatttata atgcaatcta cagaaagttt agtacttata gccttaaata
    14941 gtaaattaca aatgtatgga ggagatttag cagtaggttc aatggccata atgagcagta
    15001 taatgcaaat tcttatgctt cctaatatgg gggttacaca aggagcacaa ccaataataa
    15061 gttataatta tggttcaggt caacttgata gagtaaaaaa gacatttaag ttgtgtttat
    15121 tgtcatgttt tacatattct actattttat ggcttttatt aatgatattt cctgcatttt
    15181 ttgttagtat atttaataag aatccgcagt tacttagtat gactagttgg agtataaaaa
    15241 tatattttgc tggtgctttt atgtttggga ttcaaatagc ttgtcaacag acatttttag
    15301 cacttggaaa agcaacaata tctttggttt tagctctact tagaaagatt gtacttttaa
    15361 tacctttaat atttatactc ccaacattct ttaatgaaaa actttttgca gtaattttag
    15421 cagagccagt tgcagatata acagctgcta ctataacagc aatttctttc tttatatttt
    15481 ataaatg
//
LOCUS       DACTGW010000076        15315 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.076, whole genome shotgun sequence.
ACCESSION   DACTGW010000076 DACTGW010000000
VERSION     DACTGW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.076"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..2430
                     /gene="priA"
                     /locus_tag="I9573_001870"
     CDS             <1..2430
                     /gene="priA"
                     /locus_tag="I9573_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAT4796493.1"
                     /translation="TYQIPVFLSDVIKIGHRILVPFGKGNKPTEAFVFQFTDSLDEKI
                     KIKEIIDILDENPIFRKEDLELVYWMKNRYLCTYIECINLIYPKGYKLNNYKVVLLGE
                     SLSGLNEIELKEKISTLSDKNREIVEKVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGI
                     IKLCWEYKNHKNEKKVCYISLSLESDKIDDYIEQNKINVGSKQKEILSFLKNNENVEI
                     NDLLDLLNASKQSINSLSKKKLITLEFKDYYREPKSIYKSVLKSIKLNNEQQEAVDEI
                     KSNMFVDDKKPYLIHGVTGSGKTEVYMEIIEFALNQGLDSIFLVPEIALTPQTIDRLK
                     SRFGDLVGVFHSKLSEGEKHDVYKAVKAGKVRVLIGARSALFAPFNSLGLIIIDECHE
                     SSYKSEKNPKFNAIEVARFMALKNNITLILGSATPSIEEYYRAKSGEYKLINIKSRAN
                     DKPLPNIEVVDMKDELDKGNRSIFSLKLQKEIRYAIEENNQVILFLNRRGYANFVSCR
                     KCGYVFQCENCDISLTYHKKSNTGRCHYCGYEKEIPKECPECKSTYVKPFGVGTQKIE
                     EELKYIFPDIKTLRMDKDTTSKKGALDEILNKFKDKEADVLIGTQMLSKGLDFENVTL
                     VGILSADMILNFPDFKSFETTFQLITQVSGRAGRADKEGKVVLQTYDTEHYAIKHAIE
                     YDYEGFYEDEIKIRKAFGYSPFNNMLSVVVSGEDERLVIKNIKNMHASLIYLLEKRGI
                     NDLGFILGPNPCSISKINQNYRWQILFKDENIEINLLKGIIKYICITKRDLIFDKNIN
                     VSIDINPNSVL"
     gene            2448..2888
                     /gene="def"
                     /locus_tag="I9573_001871"
     CDS             2448..2888
                     /gene="def"
                     /locus_tag="I9573_001871"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAT4796494.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            2903..3832
                     /locus_tag="I9573_001872"
     CDS             2903..3832
                     /locus_tag="I9573_001872"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAT4796495.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTIYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            3842..4594
                     /locus_tag="I9573_001873"
     CDS             3842..4594
                     /locus_tag="I9573_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HAT4796496.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNVGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIDFLTGKNILICN"
     gene            4663..5364
                     /locus_tag="I9573_001874"
     CDS             4663..5364
                     /locus_tag="I9573_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAT4796497.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            5426..6751
                     /gene="rsmB"
                     /locus_tag="I9573_001875"
     CDS             5426..6751
                     /gene="rsmB"
                     /locus_tag="I9573_001875"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAT4796498.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            6759..7790
                     /gene="rlmN"
                     /locus_tag="I9573_001876"
     CDS             6759..7790
                     /gene="rlmN"
                     /locus_tag="I9573_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAT4796499.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGNIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            7810..8562
                     /locus_tag="I9573_001877"
     CDS             7810..8562
                     /locus_tag="I9573_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAT4796500.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            8579..10594
                     /gene="pknB"
                     /locus_tag="I9573_001878"
     CDS             8579..10594
                     /gene="pknB"
                     /locus_tag="I9573_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAT4796501.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     EEEEEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGSSNSGNSGGNSGNNGGNSGGSNSGNSGGSSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            10702..11439
                     /locus_tag="I9573_001879"
     CDS             10702..11439
                     /locus_tag="I9573_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAT4796502.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVENMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            11537..12421
                     /gene="rsgA"
                     /locus_tag="I9573_001880"
     CDS             11537..12421
                     /gene="rsgA"
                     /locus_tag="I9573_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAT4796503.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            12426..13097
                     /locus_tag="I9573_001881"
     CDS             12426..13097
                     /locus_tag="I9573_001881"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAT4796504.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            13106..13747
                     /locus_tag="I9573_001882"
     CDS             13106..13747
                     /locus_tag="I9573_001882"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAT4796505.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTVSVTAI
                     KGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            13878..14507
                     /locus_tag="I9573_001883"
     CDS             13878..14507
                     /locus_tag="I9573_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAT4796506.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            14562..15218
                     /locus_tag="I9573_001884"
     CDS             14562..15218
                     /locus_tag="I9573_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HAT4796507.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGASIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT         6184 a         1515 c         2958 g         4658 t
ORIGIN      
        1 acatatcaaa ttcctgtatt tttatctgat gtaataaaaa tcgggcacag aatattagta
       61 ccatttggaa aaggtaataa accaacagaa gcctttgtat ttcaatttac agatagctta
      121 gatgaaaaaa taaaaataaa agaaattatt gatatattag atgaaaatcc aatatttaga
      181 aaagaagatt tagagttggt ttattggatg aaaaatagat atttgtgtac ttatatagaa
      241 tgtataaatt tgatatatcc aaagggatac aaattaaata attataaagt agtcctatta
      301 ggtgaaagtt taagtggatt aaatgagatt gaattgaagg aaaaaatatc aactttaagt
      361 gataaaaata gagaaatagt agagaaagtg attgatagta aaggtaaaat taaggttgat
      421 aagttaaaat atataccaaa cttaaatagt tctttgtaca caatgaataa aaatggaatt
      481 ataaaattat gttgggaata taaaaatcac aaaaatgaaa agaaagtatg ttatatatct
      541 ttgagtttag aaagtgataa gattgacgat tatatagagc aaaataaaat aaatgtggga
      601 agtaaacaaa aagagatatt aagttttctt aaaaataatg aaaatgtaga gataaatgat
      661 ttattggatt tattaaatgc ttcaaaacaa agtataaatt ctctaagtaa gaaaaagcta
      721 ataaccctag aatttaagga ttattataga gagccaaaaa gtatttacaa atccgtacta
      781 aaaagtataa aattaaacaa tgaacaacaa gaagcagttg atgagataaa gtcgaatatg
      841 tttgtagatg ataaaaaacc atacttaatt catggagtaa ctggaagtgg aaaaactgaa
      901 gtttacatgg aaataataga atttgctcta aatcaaggct tagacagtat attcttagta
      961 cctgagatag cattaactcc tcaaacaata gataggctaa agagtaggtt tggagattta
     1021 gttggagtgt tccacagtaa actatctgaa ggagaaaaac atgatgttta caaagctgtc
     1081 aaagcaggta aagtaagagt gttaattgga gctaggtcag ctttatttgc accttttaat
     1141 tccttaggct taataataat tgatgaatgt catgagtcat catataagtc tgagaagaat
     1201 ccaaaattta atgctataga agttgctaga tttatggcat taaaaaataa tataactctt
     1261 attttaggtt cagcaacgcc ctcaattgaa gagtattata gggcaaaatc tggagaatac
     1321 aaacttataa atataaaaag tagagcaaat gataagcctc ttccaaacat tgaagtggta
     1381 gacatgaaag atgaattaga taaaggaaat agaagtatat ttagtttgaa attacaaaaa
     1441 gaaatacgtt atgcgattga agaaaataat caagtgatac tatttttaaa cagaagaggt
     1501 tatgcaaatt ttgtatcttg tagaaagtgt ggatatgtgt ttcaatgtga aaattgtgat
     1561 atatctttaa cttatcataa gaaaagcaat actggaagat gccactattg tggatatgag
     1621 aaagaaattc caaaagagtg tcctgaatgt aaaagtactt atgttaagcc atttggagtt
     1681 ggaactcaaa aaattgaaga ggaattaaaa tatatttttc cagatataaa aactttgaga
     1741 atggataaag atacaacttc aaaaaaaggt gctttagatg agatactaaa taaatttaaa
     1801 gataaagaag cagatgtact tattggaaca cagatgttaa gtaaagggct ggattttgag
     1861 aatgtaactt tagtaggtat tttgtctgca gacatgatat taaattttcc agactttaaa
     1921 agttttgaaa ctacatttca attgataact caagtctctg gaagagcagg gagagctgat
     1981 aaagagggaa aagtagtact tcagacttat gacactgaac actatgctat taagcatgct
     2041 attgaatatg attatgaggg tttttatgaa gatgagataa aaataagaaa agcatttgga
     2101 tattctcctt ttaataatat gttaagtgta gttgttagtg gtgaagatga aagattagtg
     2161 ataaaaaata taaaaaatat gcatgcatca ttgatttatt tattagaaaa aaggggtata
     2221 aatgatttag gatttatatt aggtccaaat ccatgttcaa tatcaaaaat aaatcaaaat
     2281 tatagatggc aaatactctt taaagatgaa aatattgaaa ttaatctctt aaagggtata
     2341 ataaaatata tatgtataac aaaacgagat ttgatatttg ataagaatat caatgtatct
     2401 atagatatta atccaaacag tgtattataa atttaggagg aattataatg gctttaagac
     2461 aaatagttca aataggagaa ccagtattaa gaaaaaaatc aaaaaaggtt gaaaaaattg
     2521 atgaaaagat aatacaatta ttagatgata tggcagaaac tatgtatgat gcagatggtg
     2581 ttggactggc tgcacctcaa gttggaatat taaaaagagt agtagtaata gatattggag
     2641 aagagcttat agaacttata aatcctgaaa taatagaaac atctggggag caaatagatg
     2701 aggaaggatg tttaagtgtt gttggagaag ctggaaatgt tagaagacca aattatgtaa
     2761 aggtcagagc tttaaataga aatggcgaaa caatagagtt agagggagaa gaacttttag
     2821 ctagagcatt ttgtcatgag atagaccact tagatggaat tttatttgtt gacaaaatag
     2881 aaaagtagta ggagttgatt tgatgaaaat agtatttatg ggaactcctg atatagcagt
     2941 tccatgttta caaaaaataa tagatgagaa gtatgaaata ttgggtgttg taactcaacc
     3001 agataaacca aaaggaagag gtaaaaaact aggtatgagt ccagtaaaag aactggcaat
     3061 tgaaaataat atacctgtat atcaaccagt aaaggctaga gataaagaat ttatagataa
     3121 aattaaatct ttaaatccag atgtaatagt agttgtagct tttggacaga tacttccaaa
     3181 agaaatatta gagattccta aacttggatg tataaatgtt catgtttctt tacttccaaa
     3241 atatagaggt gcggcaccta taaattgggt aataataaat ggtgaagaaa agactggtgt
     3301 tacaactatg tatatggatg aagggttaga tactggagat atgatactaa agacagaagt
     3361 aaatcttgat gaaaatataa cagcaggaga acttcatgat aaaatgatga atataggagc
     3421 agaaacttta aaagaaacat taagattaat tgaagaagga aatgctccaa gagaagtaca
     3481 aaatcatgaa gaattttcct atgcgccaat aatgaataaa tcattaggta acatagattt
     3541 ttcaaaaagt gccagagaaa tacacaattt agttagagga gtaaatccat ggccaagtgc
     3601 ttatactatc tataatgatg tcataatgaa agtttggaaa actaaagtgt tagatgaaaa
     3661 aagcacaaaa gatgtaggta caataattga tgtaagcaaa gatgggataa aagtaagtac
     3721 tatagataat gtacttttaa ttgaagaaat tcagatgccg aataaaaaga gaatgttggt
     3781 tggtgaatat ataaaaggga atactataga aactggttta gtattgggtt aggtgaaaag
     3841 tatgacagat ataaagaagt atttaacaag tgttgggatt ttgattttaa tattaggaat
     3901 tttgatagga gttgttaatt tttttataat aagtttaact caaatattct ccttgatgat
     3961 aaatattata ataatatcac taatacttat aatactatgt tcaacagcag ttacttatag
     4021 agtaattaaa ggaaagtatg ttaactcaaa tataatgaaa ataaatttta atattgtcag
     4081 tggattattt cctattatat ctttcattgc atcatcattt ggtatgtcaa aaagtgatat
     4141 tagaagaata tatataaagt taaataatga atatatatac agtaataaat ataattttaa
     4201 tccagaggat ataataattt taatacctca ttgtattcaa gagaatagtt gtaaattaaa
     4261 agttactaat gatatagata attgtaaaga atgtggaaga tgcaacgtag gagaattaat
     4321 taagctaaag gaaaaaacta atgtaaaaat atttgtagct acaggaggaa ccctagctag
     4381 aaaaattata atggataata aacctaaagc tgttgtagct gttgcatgtg aaagagattt
     4441 gacatctgga atacaagata ttaagaaaat tcctgtttta ggtgtattta acaaaagacc
     4501 taatggacct tgtgtaaata ctaatgtaga catgatggat attgaaaaag ctatagactt
     4561 tttaacagga aaaaatatat taatttgtaa ttaaactgtt tttaagttaa gtgaatatta
     4621 agaggatgta taataaaaat atattttagg agagtgttat ttatgtaccc tgtatatggc
     4681 ggtttttggg gatttgaccc aacaatggtt gtattaattc cagctatatt acttactata
     4741 tatgcacaat ttaaagtgag ttcaacaaca aataagtatt taagagtaaa tacacgtcga
     4801 ggatatactg gagagcaaac agcaagaaga gtacttgatt caaatggttt gtatgatgta
     4861 aagatagaaa tggttagagg gcatctaagt gaccattatg acccaagaag aaaggctgta
     4921 aggttgtcag aagatgtata ttatggaaca tctataactt ctgttgcagt agctgcacat
     4981 gagtgtggtc atgccataca acatgctaaa ggttatgcgc ctcttcaaat aagaagtagt
     5041 ttagtaccag tagtgaattt tgcttcaagt atatcttggt ttttaatatt tttaggattt
     5101 attatggctg gtccattttt aaaaattggt atactattgt tttcggcttc agtgttattc
     5161 caaataataa cattgcctgt agaatttaat gcttcaagta gagcaatcgt tcaacttggg
     5221 aatttaggta ttatagatga aagtgagtca agacaaagta gaagagtact ttcagcagca
     5281 gctttaacat atgttgcagc agcattggtt tcaatacttc aattacttag attgttacta
     5341 atagctcaaa gaagaaatga ctaaaagtaa agtgggttgt ttagaaacaa ccctttttta
     5401 tgtttaataa aagggagaaa tttatatgga tgcaagagaa gttggattta aagtattatg
     5461 tgatatagaa aaaaatgaca attattcaaa tattgcaata aataagcatt ttaaaaattt
     5521 agaaataagt gatatggata gaggacttgc aacagagtta atttatggag taatagaaaa
     5581 taaatattat ttagattata taatcaataa actttcaaaa ataaaggtaa aaaaaatgtc
     5641 aacttatgtt aaaatctttt taagaatggg aacatatcag attttatttt tagatagtat
     5701 atcagattat gcagcagtta atgaaactgt aaaattatct aagaaatatg ataaaaaatc
     5761 ttctggtttc ataaatgcta ttcttagaaa tgaaataaga gctaaagaga ctataatgga
     5821 cataactgag gaagatagtg taaaatactt atctataaag tattcctata actcttggat
     5881 tataaaaaac tggatagata aatttggaca agaatttgct gaagatttgt tggaagcaaa
     5941 taatgaaaag cctagtatct atataagaac gaatacactt aaaataagta gagaagaact
     6001 tattgaaaaa ttaagtgaaa tgggtattgt atgtttaaaa gttcctatgg tagaagaagc
     6061 tattaaagtc gaaaaaatga aaaatataga aaaaaatgaa ttatttaagg ctggtttatt
     6121 tacaattcaa gatataagtt ctatgatagt agggaaagta attaatccaa aagaaggttc
     6181 atttgttttg gatgtatgta gtgctcctgg agggaaatct actcacttag ctactttaat
     6241 gaacaatacg ggtcaagtag tagctagaga tatttttgag cataaactaa agcttataaa
     6301 ctctactgta aatagattgg ggcttaaaaa tgtgtttgta gaaagatttg atgcttctga
     6361 gattgatgaa aatagtataa gtaaatttga ttatgtttta gcagatgttc cttgctctgg
     6421 ttttggtata ataagacgta aaccagaaat taagtataag aaagaagaag agttaaaaga
     6481 catcacttct atacaaaaga aaattttaga aaatgcatct aaatatgtca aagttggtgg
     6541 aactttagta tatagtacat gtacaattca agatgtggag aatataaata tagttacttc
     6601 ttttgttgaa gaaaatcaca actttgaatt tgttccaatt gatacagtaa atgtagactt
     6661 agacaatcaa gataaaggat atttaaagat atatcctaat attcatggta tagatggttt
     6721 tttcatagca aaattaaaga gaataaggta gtgagagaat ggaaaagaaa aagatagtat
     6781 taaaaaattt tactgaagat gaacttaaag aatttatgaa aactatagat gaaaagccct
     6841 ttagaggaag tcaaatattc tcttggatat ataagggtgc aaaaactttt gatgatatga
     6901 acaatatacc aaaaagtttg agaaataaat tagaagaggt ttcatgtata gggaatatag
     6961 atatagaatt aaagttagaa tctaaggtag ataatacaaa aaaatatttg tttttattag
     7021 atgatggaaa tataatagaa actgtgatga tggattatga tagtagagtt actgtctgtg
     7081 tttctaacca agtaggttgt aggatgggat gtaatttttg tgcatctaca atggatggat
     7141 taattagaaa cttagagcca tgggaaattt tagaccaagt tattaaaatt caagaagata
     7201 ctggtaagag ggtatcaaat cttgtattga tggggagtgg tgagccactt gataactttg
     7261 aaaatacaaa acaattctta aaaataatta acgaaaaaaa tggtcttaat atagggtata
     7321 gacatataac tctttcaact tgtggaatag tacctaaaat gtatgagttg gcagacttag
     7381 aaatagctat aaatttagct ttatcacttc attcaccata tgatgaagaa cgaagaaaaa
     7441 ttatgcctgt agcaaatgct tattctattg aagaaatatt gaatgcatgt agatattata
     7501 taaaaaagac aaatagaagg gttacatttg aatattctct tataaaagga gtaaatgact
     7561 cagaaaaaga agcaaaggca ttagctaagc tcctaaaagg tatgctatgt catgtaaatt
     7621 taattccaat aaataaagtt gaagaaagag aatacgaaaa gccagataaa gcatttattt
     7681 ataaatttag ggatagtttg gagaagaaca atatacctgc cacagttagg atgtctatgg
     7741 gttctgatat tagtggagct tgtggtcaat tgagaagaaa atacaagtaa tttgtaagga
     7801 ggaagtcgta tggtttatag ttgtgcctcc catataggaa aaataagaaa aaacaatgaa
     7861 gactattgtg agggagaagt tatagataca gaacatggtc caataggaat atttgccata
     7921 gctgatggaa tgggtggaca taaaaaaggt gaagtcgcca gtaaattagc agtagaaaat
     7981 ataattgatt ttctaaagga aaacttatta caacatgata atgtaaaaat agactatata
     8041 gatgatatat taaaacaggc ttataataat gtaaactcaa ttgtgcacaa aaaatctatg
     8101 gaagatatag aatttgaagg aatgggaact acattagtta cggctatagt gtataataat
     8161 gtcttatatg tggcaaatgt tggagatagc agatgttatt tgttaacaga tgaaaaattt
     8221 gataaaataa ctatagacca ttccgtggtt gaagaactta tgatggccca tgtcattaca
     8281 gaagaagagg caagaagaca tccacagaga aatagaataa ctagagcaat tggaacagat
     8341 gatatggttg ttgtagatat ttttaagaaa gaaatcaaaa aaagtgatat aatacttctt
     8401 gcgacagatg gattaactgg gtttattgat gatgaagata taagagattt aatattagat
     8461 tatgaatatg agagaactag taatataagt gaagaattga taagtatggc caatgatgtt
     8521 tctggtaaag ataatgtttc ggttatagtg ataaaagtat aattaggacg gtgatattgt
     8581 gggagataca attttaggaa atcgatatga aatcatcagg aagattggtg atggaggaat
     8641 ggcctttgta tatgaggcta aagacagatt attaaacagg actgttgcac ttaaagtact
     8701 tagacctgaa tttgtagatg atgatgaatt tctaacaaag tttaaaagag aggcagaagc
     8761 agtagcaagt ctttcacatc caaatatagt aaatgtttat gatgttggtg aagatggaaa
     8821 agttcactac attgtaatgg agtttgtaga tgggaagaat ctaaaagaga ttatacaaga
     8881 tgaaggtata ttagatgaat atactgcatt agatataaca aaacaaatag ctatggcact
     8941 tagtgcagcg cacaaaaagg ggattataca tagagatata aaacctcata atattctcat
     9001 atcaaatgaa ggaagagtag taaaagtggc tgattttggt atagccaaag ctgtttcaaa
     9061 ttcaacaatg actaatattg gtagtataat aggttcagtg cattattttt caccagagca
     9121 agctaaaggt aaatttgtaa ctaataatgc tgacctgtat tctctaggta tagttttata
     9181 tgaaatgttg ataggaaaag ttccatttag gggagatagt ccaatttcta tagcacttca
     9241 acacattaat gatgatatag actttacatc agaagaaaaa gttaggattc ctcaaagtgt
     9301 aagaactaca ataaaaaagc ttacagaaaa atctagcgca gatagatatc aaactgctga
     9361 agaattgata gaagatatag agtatataga aaaaaatatt gatttagatt ttataaaaga
     9421 atatgatgat tttgcaacta agaaaattga tgaaaaagaa attaataaag ttgtaaatcc
     9481 aacacttgca aaaccagctc ctgaaaaagt ggttaagcct gttgaagtag ctgatttgga
     9541 tgatgatgaa gattactatg atgattttta tgaagaagat gaagaagaag aagaagaaga
     9601 ggaagagata atgagagcta aaaagaatca gagacctaag agtactccaa gtaagagaac
     9661 taagaaaaag aagaaaaaac aggaaagccc aaaatctaga agaagattaa aagtaattgc
     9721 ggctgttttg atactgatat tgtgtgctca agtattctta gcatataagt ttttatttgc
     9781 tggagggttt ggcaataaat ctttgactgt tcctaatctt gtaaatatga cattagaaga
     9841 agctcaaagt gctgttgaaa aagaaggatt atacctgagt gtaaaaagtg aagagtataa
     9901 tagtgaagtt gatgaaaatt gtataatatc acagacacct gaaggtggaa gtacaaatgt
     9961 caaaaagggt gatactataa atgttgtcgt aagtaaaggt tcaagtcagg catctgttcc
    10021 taatgtggtg ggacttactt tgagtaatgc aaaacaactt attgaagaaa ataacttaaa
    10081 agtgggaact gttaagtatg aatatagctc aatatataaa gaaggtacag ttttaagtca
    10141 gagtccaggt gctggctcat caagagctca ggaaggtgat gaggtaaacc tatatgtaag
    10201 caaaggttca gaaaaatcaa atacacagac tccaacagta ccagataaaa aaccaacaac
    10261 accagagaat aatacaccaa cagaacctgg ttcaaactca gggaacagtg gtggaaattc
    10321 aggaagttca aactcaggaa atagtggagg aaactcaggc aataatggag gtaactcagg
    10381 aggttcaaac tcaggaaata gtggaggaag ctcaggaggc tcaaactcag gagataatgg
    10441 aggaagttca ggaggttcaa actcaggaga taatggagga agttcaggag gttcaaactc
    10501 aggagacaat ggagatagtt caggaggctc aaactcagga gataatggag gaacttcatc
    10561 aggaactggc gcaaacattg gtaaaacaga ataaatatta ataaaataag agctgtcctt
    10621 ttatagaaaa tattttcttt aaagggcagt ttgttttgta taaaatactg aattggttat
    10681 aatataagga gaaaagtatt tatgaagaag aaaaccgttt taattacagg aggagctaga
    10741 gggattggta aagctatgtc taaagctttt gcaaaagaag gttacaatgt attagtaaat
    10801 tttaataagt cagaaaatga agctaaagag ttgtatacta tattaaatga aaaaaatttt
    10861 tctgtaaaac tttttaaggc tgatatttca aatagagaag atgtagagaa tatggtagat
    10921 tattgtataa aagaatttgg aggattggat gtacttgtaa acaatgctgg tgtaagtcaa
    10981 gataagttgt ttacagatat aaccgatgaa gattgggata atatgatgaa tattaattta
    11041 aaaggaagct tttattgttc gcaagttgct ttaaaataca tgatatcaga aaaaaaagga
    11101 aatataatta atatatcttc aatctggggt atatcaggtg cgtcttgtga agtgcattat
    11161 tctataacaa aagcaggtat aataggtatg acaaaggcat tagctaaaga agtaggacca
    11221 tcaaatatta gagtaaatag catagctcca ggtgtcatta atactgacat gttatctgga
    11281 tataatgagg aagatataga cgctcttgtg gaagaaaccc ctctaatgag gctaggaact
    11341 ccagaagata tagcaaattg tgctattttc ttggcatcag acaaatcaaa ttttatcact
    11401 ggtcaggtaa ttagtccaaa cggaggattt gttatttaaa aaaaatgtgg tattatataa
    11461 aaataggaaa gaataacacg atatactatt cataaacagt atgtgtttat ataaatttta
    11521 taaagggagg catttgatgc tagaaggaaa aattataaag gggattagtg gattttatta
    11581 tgtagatact tataatggta tttatgaatg taaggctaga gggatattta gaaagcagaa
    11641 aataacacct ttggttggtg atagagtcaa aataagcatt gttgatgaag atgaaaaaaa
    11701 gggtatctta gaagaaatcg acagtagaga tacagaactt ataagacctc ctattgcaaa
    11761 tgtagataaa gcattaatag tattcgcaat aaaaaatcct aaaccaaact tatctctttt
    11821 agatagattt atagttttgg cagaaaaaga aaatttagaa actgtaataa tccttacaaa
    11881 agcagattta gatgataatg atacattaga aactgttaaa aatatatatg aacttagtgg
    11941 ctataaagtc attcctgtaa gcaatattac taagttaaat atagataaag taaaagaaga
    12001 attaaaagaa aatgtagtag tgtttgcagg accatctgga gttggaaaat caagcttatt
    12061 aaatgaaata gatgaaaatt ttaaacttca aacaggagtg gtaagtgata aaataaaaag
    12121 gggaaaacat actactcgtc acgctgaact tttaaaattg gaatttggtg gaatggttgc
    12181 agatacacca ggttttagtt cccttgcact tgaagatata gaggaagttg aacttaaaga
    12241 ttattttata gaatttgata aatttaatga ttgtaagttt ggttcaaagt gtattcatga
    12301 aaatgaacct aattgtgcta taaaagaagc tgttacaaat ggagaaatat ctaaagaaag
    12361 atatgatagt tatatacagc tgctacatga aataagacaa aataatagta ggaggtatta
    12421 gaaaaatgat taaattagca ccatcaatat tatcagcaga ttttgcaaaa ttattagaag
    12481 atgtaagaaa agtagaaagt gcaggatgtg aatatcttca tatcgatgta atggatggac
    12541 actttgtacc aaacattaca ttaggaccac ttgtagtcaa aagtttaaag aaagaaaata
    12601 taaatatggt atttgatgct catcttatga tagaaaatcc agaccagtat attgaagaat
    12661 ttgtaaaagc aggatgtgat ataataactg ttcaccagga ggcttgtgtt catctacata
    12721 gaactataca aaatataaaa tcacatggaa taaaagctgg tgtagtactt aatccagcta
    12781 caccagtaga tacgattaaa catgtactac ctgatttaga tatggtatta ttaatgtctg
    12841 ttaaccctgg atttggagga caatcattta ttccttgtgt tttagataag attaaagagt
    12901 taaaagctat tatagatagt caaggattaa acattgatat agaagttgat ggtggtataa
    12961 gccctaaaaa cgtagccgaa gttgtacaag caggagctaa tgtaatagta gcaggttctg
    13021 caatatttgg aagtgatgac attcaagaaa cagttaattt atttagaaag aatgcttctc
    13081 ttgaagaatt agtttaaatt taataatgaa aatctgtatt gttttaaatg gcgaaattga
    13141 agattacgaa gttacaagag atattataat taaggaatgt tatgactgca taatatgtgc
    13201 agatggaggg gcaaatcaca cttataaaat ggaaataatg ccagattata tattaggaga
    13261 tttagattca gtagaagaag aaaagataaa cttctataaa aataaaggtg ttaaatttga
    13321 aaaatttcca tctaaaaaag atgagactga tacagagctt tgtctttttt tagctaagac
    13381 acttaaagca aatcatattg atttttttgg tgctttaggg ggaaggattg accacactct
    13441 tgcaaatata aagctattat attatttaaa agaagatggt atatattcta ggatactttc
    13501 tgataaagaa gagatgtata tagttgaaaa tgaggaaatt tcattgtatg gaaacccagg
    13561 ggacactgtt tctgtaacag ctataaaagg tgatgctaag ggtgtaacac ttactggctt
    13621 agaataccct ctcgataatt attatatgaa atactctgta cctattggaa tatcaaatgt
    13681 tatgttgagt aattcttgta agattaaagt agagcaaggc tgtgtacttg tagttagaaa
    13741 tttataaaat ttgattttga caattggttt tatattgtag agcagataca atcaagtaaa
    13801 atatatttat gattgtatct gctttttagt atataattat aaatatataa taaactttga
    13861 cataaggggg attttttgtg gtagatagta ttatttttga tttagatgga acattgtggg
    13921 attcaactga agaagtatgc aaagtctggc aggatgtttt aagtaaacat aaggaaatag
    13981 aattaagtgt cacaaaagat ttatttagaa gtcttatggg gctttctttt gaggaaatag
    14041 caaatcgttt atttccaaat ttaagtgaag aagaaagaat gaatttattg aatgaatgtt
    14101 ctttgaaaga atgtgactat ttatctgaaa atggagctaa attatttgat gatatagaag
    14161 atactcttaa attactatct aaaaaatata aattatttat agtaagtaat tgtcaagctg
    14221 gatatataga atctttttta aaagcacata aactagaaca gtattttata gattttgaat
    14281 gtcctggtaa tactggtttg cataaaggag aaaataataa acttataatt gaaagaaata
    14341 aattaataaa tccaatctat gttggtgata cgcaaggtga tgcaaatagt gctaagtttg
    14401 taggtatacc ttttatatat gccaaatatg ggtttgggaa tgtagatgta tatgattatt
    14461 ttatagattc atttaaagat ttgttagaac atgatatatt aaaataagat gagtttaaaa
    14521 attatattaa aataataaaa aagttatggg gggatttaaa aatggaatta caagatacta
    14581 tttttaaaag acaaagtgta agaaagttta aaaatcaaga tgtatcagat gaagatattt
    14641 taaaaatgat aaaagcagca ggtgcagcac catcaggcaa gaatattcaa aactggcatt
    14701 ttgttgttat aaaacgccgt gatttaatgg aaaagatagc agatgtgata actaaaaagc
    14761 aacaagaaat acttgtggaa atggataaag tatcagtgga taaggctaat agatttagaa
    14821 aatttgttaa aaactttaca ctattttact taaaagctcc agttttagta ttagttttta
    14881 caaaggtata taatccatca ggatattatg aattagagct tatagatgca cctaaggaga
    14941 ctatagataa attatttata agaaatccag gtatgcagag tctaggtgct tcaattgaaa
    15001 actttacact atcagcaatt gaacttggat atggttcttg ttggttgaca agtcaaaact
    15061 atgcagcaga tgagatagaa gctgttttag aagcagaaac tggttttgaa aagggagagt
    15121 attttttagg tgcaatgtta gctcttggag tgccagagga caatttgaag agtccatcta
    15181 aaaaaccagt agaagaaata tgtacattta taaaataaaa gtcaaggaca ttttaaaatc
    15241 aaagtaaatt aaaaacaaga taaattaaaa ataaatattt aaaaaattaa aaagtaggtt
    15301 ttattgatat taatt
//
LOCUS       DACTGW010000077        15266 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.077, whole genome shotgun sequence.
ACCESSION   DACTGW010000077 DACTGW010000000
VERSION     DACTGW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.077"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            196..1113
                     /locus_tag="I9573_001885"
     CDS             196..1113
                     /locus_tag="I9573_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HAT4796508.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYKSEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSIIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            1191..1928
                     /gene="cas6"
                     /locus_tag="I9573_001886"
     CDS             1191..1928
                     /gene="cas6"
                     /locus_tag="I9573_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HAT4796509.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KAYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKIVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            1934..3811
                     /gene="cas8a1"
                     /locus_tag="I9573_001887"
     CDS             1934..3811
                     /gene="cas8a1"
                     /locus_tag="I9573_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HAT4796510.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQIMFNDYLRQIIYFGELADLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTSINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLTNNILRNKVKNDMESNNKSNISGIDCFRVVQLRALINSYKKG
                     VYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            3813..4727
                     /gene="cas7i"
                     /locus_tag="I9573_001888"
     CDS             3813..4727
                     /gene="cas7i"
                     /locus_tag="I9573_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HAT4796511.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            4729..5430
                     /gene="cas5b"
                     /locus_tag="I9573_001889"
     CDS             4729..5430
                     /gene="cas5b"
                     /locus_tag="I9573_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HAT4796512.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            5441..7762
                     /locus_tag="I9573_001890"
     CDS             5441..7762
                     /locus_tag="I9573_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HAT4796513.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQREFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIIGVGEANDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIHENKHTKQIFGKGVLISNEIDQFI"
     gene            7810..8328
                     /gene="cas4"
                     /locus_tag="I9573_001891"
     CDS             7810..8328
                     /gene="cas4"
                     /locus_tag="I9573_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HAT4796514.1"
                     /translation="MPIDLEVLKVQGVKINYYYVCKRKLWLFSKGITMEQNSSRVLSG
                     KIVHEDSYKRMKNKEMLIDDILKLDIIKDEYVKEVKISSKMKEADEMQLYYYLYYLKN
                     SMGIEKKGTINYVKEKKQEELILTEEKEKIIENTLININEINMQLSPPKIEKKHYCTK
                     CAYYEFCFVKEE"
     gene            8331..9311
                     /gene="cas1b"
                     /locus_tag="I9573_001892"
     CDS             8331..9311
                     /gene="cas1b"
                     /locus_tag="I9573_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HAT4796515.1"
                     /translation="MYKDYYIISNGNIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNTLMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            9314..9580
                     /gene="cas2"
                     /locus_tag="I9573_001893"
     CDS             9314..9580
                     /gene="cas2"
                     /locus_tag="I9573_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HAT4796516.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITDGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   9764..11248
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9764..9792
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            11438..11866
                     /locus_tag="I9573_001894"
     CDS             11438..11866
                     /locus_tag="I9573_001894"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAT4796517.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIEPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(12322..13101)
                     /locus_tag="I9573_001895"
     CDS             complement(12322..13101)
                     /locus_tag="I9573_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796518.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMSSEVSSIRCCNCDELLEDITLNKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            13425..13970
                     /locus_tag="I9573_001896"
     CDS             13425..13970
                     /locus_tag="I9573_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAT4796519.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            14002..15138
                     /locus_tag="I9573_001897"
     CDS             14002..15138
                     /locus_tag="I9573_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HAT4796520.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CNLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIERADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     PILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKTKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIERYNIFGGALAYGH
                     PYGASGAIITLHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
BASE COUNT         6313 a         1397 c         2503 g         5053 t
ORIGIN      
        1 tttttattgt attaactatt aaaattttgt acaaatataa aatataatat ggtaaatgtc
       61 aaaattataa aaaaattcta cttatataaa atataaagag gttatatgta catatgtgta
      121 gaaataaata ataaaaatat caattaatgt tattaggggg ggttactcat tagtaaatta
      181 gctaatacac ttagaatgat gatattaatt caaagcagag gcaaaatgaa atgcaaagaa
      241 ttatcagaag agttggaagt aagtgaaagg caaataaaaa gttacaagat gtatttagag
      301 caagctggta tatttataaa ttcaacacct gggatttatg gtggttatga aatagataaa
      361 tgtaatagca tatcattaat taaattatta gatagtgaag tttcaattct agatatgata
      421 aattcacagt tagagtataa taatgatatt tacaaaaatg aatttaataa tattgtagaa
      481 aaaataaaag cagtattaaa tacaggagaa aaaagcgata catatatgga ttattttaca
      541 gttcaagcac aacgtaattg tgattataag tctgaaaaaa ataagtgtaa tgaaataatt
      601 agagcatata ccacaaaaca caaattttgg atagaatatt attcattaaa ttcaggaaat
      661 agtgaaagaa ttgttcatcc atatggttta tttaattata aatcagatac ttatatggta
      721 gctttttgtg aaaaaagatt taagtttata gactttaaat tatgtagaat taaagattat
      781 aaggttttag aagaaaaata taatgtagat aaaagtttta gttgggatga atatagtaaa
      841 aatagtatag gaatttataa aggagaagaa attaatgttg ttataaaaat tagtcatcca
      901 ttttcaataa ttataaagga aaaagtttgg gttaataatc aacaaatcat agaatatgat
      961 gataaatcta taatgtttaa agctaaaatg agaggttatg aggagataaa aagttggatt
     1021 ttaagtatgg gagcttatgt tgaggttgta gaaccagata gacttagaaa tgatatattg
     1081 tcagagattg aaaaaatgaa aaaaatttac tgattttttc aaataattta tacttggacc
     1141 aatatacttc acggtctagt gttaatatat taattgagag aggtgatatt atgaaaatga
     1201 aaattgaatt tagtacaggt tgtataccaa tatcttataa ttcgctcttt atgagtatta
     1261 taaaagaagc aataagaaaa tctaatgaag attactataa aaacatgtac tattataaag
     1321 aaaagaataa taaaaaaact aaaaatttta cattttcggt ttatataaaa aagtatagta
     1381 ttgaaggtga caattttatt atagaagata aagtaatatt aaatataagt actccagatt
     1441 tagaattggg ttttcatata tacaatgggt taatgacttc taaaaaatgt ttatataaag
     1501 cctatgaact tacaagaatt agaatcgatt tatcaagaga aaagaaggta acagaagaaa
     1561 gggtactatt taatgcattg tctccaatct gtgtaaagtc taaagaagga aaattcttgg
     1621 agataactga tgatagatac atagaagaac taaactatat aactaatgaa gtggttaaaa
     1681 attatagggg aaatggatta aaaagaaagt tagaatttga aaatataggt ctcaaaaaga
     1741 tagtagttaa agagagctta agagaattta aaaaaataac aggtaaagag tatcaatata
     1801 ttaatggtta caaaggtaaa tttatattga aaggtgatat tgatgattta aatcttatct
     1861 acaatctagg aattggtttt agacgtgctc agggatttgg agatgtagat attttagaat
     1921 ggaggtgaga taaatgaagt taagagttta tagaggagat tggtttttta atatgggtat
     1981 agttggattt ttaaatataa taaagaaagc agataaacaa gctgaaatat ttattatgga
     2041 agattatatc gaatttgata gcttatttct tgaaaacttt catgagtatt attttaatta
     2101 ctttatggat gaatatgatg tatcaaaaag aattaaaaaa aatattgatt acagtataaa
     2161 ttttattaaa tctaagcctg atagaatcaa agatggaatt aagaaaatta aagatagtgt
     2221 gaaacaacaa aatgataaga ttaaaaaatt tgatgaagaa aaatttaacc tgataaaaga
     2281 gaagctagat agcatgtcta agataaaaag ttatgatgaa tttgacagtt tagaagcttt
     2341 agtagatgag actatagata tatttaaaat aaaatcaata aatgacaggc ttacagcaaa
     2401 tctttataaa tatgtagttc aagataatta ttttggacaa gttagttttt ttaatgttgc
     2461 taaggcgaaa ttagatttag atggattaaa acagataatg tttaatgatt atttaagaca
     2521 aattatatat tttggagagt tagcagattt attagaagag aatgactatg ataagttgaa
     2581 aaactattta aatgatagat taaatagtat agctaaggat ataaatgaaa aaaaagttag
     2641 taagtctagt ataaatacta tagaaaaaat tatgaaggaa ataaataaaa actttataaa
     2701 gaaaaataaa agcatagaag atataaaaga gtatatggat agtttagaag tatgtgaaat
     2761 gtgtggttta tataagggaa tattagatga atactcagaa agtaattttg ctccacttgg
     2821 agtaagcacc aataatgcta ggaatatgtt ttggaatcag gcgtatgtac catcaatatg
     2881 tgatatttgt aagctaatat tgttctgtac tccagcaggg gctacttata caagaaagaa
     2941 ttatttaatt aatgaagaaa atgagtttta tttatttgtt aacatggata cttctataaa
     3001 tgagcttttt gaaagaaata atagtttaaa agctcaaaaa agtgaatatt cagattccaa
     3061 agatgaaaat ccttttaatc agcttataaa aagtatagta gaagaaaata cattaaaaag
     3121 tgaatggcaa cttcgtaata tactttttgt ggaatttaaa gcaagtatag attctaaaaa
     3181 atgtaaaata aattatttta atattccaac ttatttagca aaatttttta cagacaagta
     3241 tgcaaataaa aaaatacaaa gtatctatga ttacaagctt aaatcaaatg tactagattt
     3301 actcttgaaa aatagggact tgaagcatct aactaataac attttaagaa ataaagttaa
     3361 aaatgatatg gaaagcaaca acaaatcaaa tatatcagga atagattgtt ttagagtagt
     3421 ccaattaaga gcattaataa atagctacaa aaagggggta tataaaatgg atagtaaaaa
     3481 tttggagaag aatgatgaaa agctgagaat aatttattat ttaggttgtg atatacatga
     3541 ctattttgtt aataaaaatt ctaaaaataa aatagatgga gtaagctata aacttttaaa
     3601 ttcagttaaa gtcggaaata aaagtgattt tatggataca attataagag tttttatgtc
     3661 agctgaaaaa cagattccag catttatttt agatatagaa attgaaaaag atttagactt
     3721 tgaatctata ggtcatgctt ttatatctgg attaatttct ggaaaatatg aaggaaaaga
     3781 caaactacca aacgaaaagg aggaaaaata agatgaaaag agggttaaca tttacagtaa
     3841 tagcacaggc tcaaagttta aactatgggg aagggatagg taatatatca gaattaaaaa
     3901 aattaagtag agatgatgga aatatatata catttgcatc tcgccagtgt ttaagatatg
     3961 acatagtacg attagcagca gaattgttta attggaattt acaggttgtc gataaagaaa
     4021 aaggtactat tcaatttaaa gacaatataa gtataagaga atcagaagaa atggatttat
     4081 ttggatatat gaaaacaaat aaaaaagatg agaaagataa aaaaggaggt tctttgacta
     4141 gagaagctac tgtaagatta agtaatgcaa tttcccttga gccatataga agtgacatgg
     4201 actttttaaa taataaagga ttagcagata gaataggaga acatcctaat cttgctaaca
     4261 tagagcaaca tttaagttat tatacatata cagtgactat agatttatct aaaataggta
     4321 aggatggaga tatagaactt gataataaag agaaatgtag aagagtagta gaatttttag
     4381 aaataataaa agtattgaat agaaatatta gaggtagaca agaaaattta tctcctctat
     4441 ttgtagtagg aggagtatat gaaatagcaa atccattttt cttgggtaga attaaattaa
     4501 aaggagataa aaatggattt aaaataaata agcaagctat agaagaagta attcaaggta
     4561 cttttttagg aaaagattta aaagaattta cctatgttgg aatggtagat ggggtattta
     4621 taaataaaga agaatttaag ggattgtttg aagataattt tttaagtgta gataaatttt
     4681 ttggacaact agttaaagaa gtaaaggaat attatggggt gaattaatat gaaggttttg
     4741 aaattagatt tatttcaaga aactgcatgc tataaaaaac catttgctat gaagattaca
     4801 gagacatatc cactgcctcc atattctaca ataaatggat taattcacaa aatattaaat
     4861 gctacagaat atataccatt taatataagt gtacagggaa catatgaaag tatatttaat
     4921 aattaccaga caacatattt ttataaaaaa gatagtatta catctatgcc aatgaatagt
     4981 catatgctat tgaatgtaaa tttgataatt catataggtg gggattttga tttattggaa
     5041 gaaatatatg atagtttatt aaattatgac gagcacttat cattaggtag aaaagaagat
     5101 ttagtaagaa ttaatgatat tagatttgta gaagtcaata aatttgaagt agatgaggat
     5161 atacaattag atgattatga aaattataag ttatcagaat gtaatataag aatgcctata
     5221 tatataccaa aaacaagttt acaagataca gatatagatg gtataagtta cagactcaat
     5281 aattactatg ataataatgc tgtggaagat aaaaaaaggg tttggaataa ggtagatagt
     5341 tattatgttg aggatggaaa tactatatca attggagata tattattaga taatgaagga
     5401 gatttattat attttaataa tataaattag gagggagagt atgttatatg ctaaatctaa
     5461 tccagtggaa acacttagag aacatactga tgaactttta aaacaaatga atgtattaag
     5521 agaaagttat ggaaaaaata taaatagctt agattttcta gaggaagaga tattttggca
     5581 attacttgac tttgttatag aatttcatga tattggaaaa gcattttcac cgtttcaaga
     5641 gttaataaaa agcagaatgg atactaaaat aaatgaacct aaaatagtaa ctcatttaga
     5701 aaataatgta ggccataatt atctttctcc tgcatttatt gattatagtt atatagacag
     5761 gaaaaaaaat aaagaactta gagcagtatt aaatcaagtt attgtatatc atcatgaaag
     5821 agatatcttt atagataaag attttaaaaa tttaatacaa aaaatattag atgaagattt
     5881 aataaataaa gtttatgaac tacagcgtga atttaaagtt agatatccaa taaaaacaga
     5941 aaaattgagt aaagtatatc tacaaagtgt agaaaaaaga atagataaaa atcacaagta
     6001 ctataattta tatattatgt taaaaggaat actacataga ctagaccata gtgcttctgc
     6061 tcatgaagtt gtggaatgca ataatgttat aaatatagga gaacagacag aaaattattt
     6121 aatgaaagag tttggtagtt tgagagaggc tcaatcattt gctaaatcta atagaaataa
     6181 gaatataata ctgattgcat ctacaggaat gggaaaaaca gaaactgctt tgatttggat
     6241 agataaggat aaagcatttt ttacactacc attaagagta agtataaatg cattatttga
     6301 tagggctaaa aatataattg gagtaggaga agcaaatgat acattcttag gtctgttaca
     6361 ttcaacagca atagattatt tagaagaaag taatcaggaa aattcaagtg agattgtaga
     6421 tttagcaaag ttattatctt gtaaactgac attttccaca atagaccaaa tatttaaatt
     6481 tccttttttg tatagaggct atgaaaaagt gtactcaaca ttagcatatt caaaagtagt
     6541 aatagatgag atacaagcat attcaccaga aatagcagct gtattggtaa aaggtataga
     6601 aatgatacat aagataggtg gcagatttat gataatgaca gctactatgc caaccatata
     6661 tatagatgaa ttaaaaaaaa gaggtgtaat gaatagtaac ttagctgact tgacatgtaa
     6721 cacagaaaaa ataagacatt gtgtgtctat tgtagaaaat tcaatagatg aaaacttagg
     6781 taaaataata caaagtggaa tgaataaaaa agttcttgta atagtaaata cagttaaaag
     6841 tgctgtagaa aaatatgagt taatagaaga aatagttaaa ccaaaaggaa tagatgtcaa
     6901 cttaaatctt ttacattcta tgtatataca agaagataga gcaaaattag aaaaatatat
     6961 aaaagaattt gcagatagtg atagtaatgg tatttggatt actacacaat tagtggaagc
     7021 atcccttgat attgattttg atgagcttca tacagaaaat tcaacacttg acagtctatt
     7081 tcaacgattt ggtagatgtt atagaagtcg tgagtatgaa gaaaatagcc caaatatatt
     7141 tatatataca gaaaaagcta caggaatagg tagtatttat gataaggata ttgtagaaaa
     7201 aggacttgag ctactgaaaa cttttataaa tggaaaagaa tgtgtgaaga tgaaagaaaa
     7261 atataaggta gaaatggtta agatactata ctctaaagaa agtctagaag gtacagcttt
     7321 tgaaaagaga tttaccagtg ctttaaatat tttagatact ataacgccat atagtttagg
     7381 ttctaaagat gcacaaaata tacttagaaa tattgaagga tatacagtaa ttccagagga
     7441 tatatataat aaaattgaag aaactttaat caaagattat gaagagttgg gacaagagct
     7501 tataaatgca tatagtaatg atgatagaca attgataaat aaaataaagt ctaagagaaa
     7561 aaaagcaaga agagagataa ttaaaaaaac tgtaaattta ccaatatata aagctaaaca
     7621 aaatgtaatt gacatacagg tgaaaggtct tgaagattta aaaatacttt tatataaata
     7681 tgatatacac gaaaataagc atacaaaaca gatatttggt aaaggtgtgc ttataagtaa
     7741 tgaaatagac caatttattt gatttttata ttaacaatgt ggctaaaatt tactaaagaa
     7801 ggtgattttg tgccaataga tttagaagta ttaaaagttc aaggcgtaaa aataaattat
     7861 tattatgtat gtaaaagaaa actatggcta ttttctaaag gaataactat ggaacaaaat
     7921 agtagtagag tattgtctgg aaagatagta catgaagatt catataaaag aatgaaaaat
     7981 aaagaaatgt tgattgatga tatcttgaag ctagatataa taaaggacga gtatgtaaaa
     8041 gaagtaaaaa taagttcaaa gatgaaagaa gcagatgaaa tgcagttata ctattactta
     8101 tactatttaa agaatagtat gggtatagag aaaaaaggaa ctataaatta tgttaaggaa
     8161 aagaaacaag aagaattgat attgacagaa gaaaaagaaa aaattattga aaacacactt
     8221 ataaatataa atgaaataaa tatgcagtta tctcctccca aaattgagaa aaaacattat
     8281 tgtacaaaat gtgcttatta tgaattttgt tttgtgaagg aggagtaaca atgtataaag
     8341 actattatat tataagtaat ggaaatataa aaaggaaaga aaatacgatt tactattaca
     8401 atttagaagg tgagaaaaaa atattaccag tagaacaagt tgatgatatt catatatatg
     8461 gagaagtaga tttaaacaca aaacttataa attacataag taaatatggg gtgatgcttc
     8521 atttctacaa ttactatgga tattattcag gtacatatta tccaaggaag aaaaatgtat
     8581 caggattcag tttagtatgt caatcagcct gctatttaga taaaaatgaa agattatact
     8641 tagcaaaatc ctttgtggaa agtgcatcat accatatttt aagaaatatg agatattaca
     8701 aagttgatga aagtttaatt gatactataa aggatgaact aaatgatttg aatgatgtca
     8761 aagaaatatc agaacttatg ggtaaagaag gcagaataag aaaaacatac tataaatcat
     8821 ttaatcaaat attaaaagat gattttgaat tttataaaag agaaaaaaga cctcccaaag
     8881 acccagtaaa tactcttatg tcttttggaa atagcataat gtatacgaat gttttaagtg
     8941 agatatataa gacacaactt gaccctacaa taagcttttt acatgaacca tcaacaaaga
     9001 gattttcttt aagtttagat attgcagaaa tttttaagcc aattatagtt gacacaataa
     9061 tttttagcct gattaataat aaaagaatta ataagaatga ttttgaatat caagacaaaa
     9121 tttgttattt aagtgaaaaa ggaaagaaga aatttttgca agaatttgaa aataagatgc
     9181 agacaactat aaaacataga aatctaaata gaaaagtttc atatagaatg tttataaggt
     9241 tagagtgtta taaattaatt aaacatttta taaatgatga aaggtacaaa gttttaaagg
     9301 tgtggtggta agtatgtttg ttattgttac ttatgatatt gttgaagcaa ggtcgttaaa
     9361 tagaattaga aggatactta gaaaatattt gacttggacg caaaattctg tttttgaagg
     9421 caatattact gatggaaagt tacataaatg tatttctgaa atagaaaata ttattgataa
     9481 tagcgaggat tcaatctatg tttatgagat aaaaaatcct aattcaatta aaaagaaatg
     9541 ttatgggatt gataagtatt ctgatgaaat gtttatatag gtttgcagtg agcgatattt
     9601 atgctaaaat aggtgttaac agttggaata taagggattg aaggtgtatg ataactgtta
     9661 tcaattgcac tactgctcgc tcactgcaaa ttttgatgtt tttattgaat tataattgct
     9721 tgattgaagt attttcaatg tattcaatta tacctatttt ggggttttat attaactaag
     9781 tggtatgtaa atttaaaggt gcaagtctta atacttgtta catgagttgt tttatattaa
     9841 ctaagtggta tgtaaataac aaagaaccat ttgaatcacg aaaactaaaa aacagtttta
     9901 tattaactaa gtggtatgta aatttattaa ccttatcaga aacctgacca tcccaattat
     9961 agttttatat taactaagtg gtatgtaaat tccatttgtc taatgcttct aactcttttt
    10021 ttatacgttt tatattaact aagtggtatg taaattccaa aatgggcttg gagttaagag
    10081 ttgtataaga aacgttttat attaactaag tggtatgtaa attaattaca cactcccttt
    10141 taacgaacat attttagatt gttttatatt aactaagtgg tatgtaaata gactcgacat
    10201 agaatcggtt actaaatccg agggtttcag gttttatatt aactaagtgg tatgtaaata
    10261 ctaaaccttt ttgtttactt ttatttctca ttttacgttt tatattaact aagtggtatg
    10321 taaatccgga tttttctgct tcgactgttg ttattgagac ttgttttata ttaactaagt
    10381 ggtatgtaaa tagtaattcc atgcaatctt ttatattgct ttttaacctg ttttatatta
    10441 actaagtggt atgtaaatag atatacaagg tgaaagtgtt gaaatttcaa tcttgtttta
    10501 tattaactaa gtggtatgta aatattctta gatgttataa tttgtgttgc atttaaatcg
    10561 ttttatatta actaagtggt atgtaaatac aatattattg ttcatttcat ttaataattc
    10621 tagtagtttt atattaacta agtggtatgt aaatttgttt atacctttaa tctttaaaat
    10681 taaatataaa agttttatat taactaagtg gtatgtaaat cattgtatac taagcttgat
    10741 atagtgcaaa atgaaacgtt ttatattaac taagtggtat gtaaatacta attgggttaa
    10801 acaaatggat ataactaaat tagttttata ttaactaagt ggtatgtaaa tccaaccctt
    10861 cgcacgaatc cgtgcgacga aatataaagt tttatattaa ctaagtggta tgtaaattgt
    10921 aaaaatattg cttagcatat tgatatcact aactgtttta tattaactaa gtggtatgta
    10981 aatacaagag gtttttcagc aatatatgta cttctataat gttttatatt aactaagtgg
    11041 tatgtaaatt catcaatatt aattttggga atatcaacat tttttgcgtt ttatattaac
    11101 taagtggtat gtaaatgcga taagagattc gagtcttttt actgggctta atcgttttat
    11161 attaactaag tggtatgtaa ataggataaa gaaaagactc acacaagaca cagtgtcagg
    11221 ttttatatca actaagtggt agataagtag aatattagca atatcaacga gtatttagaa
    11281 acttatttta tattaataaa gcagtatata aaattgtaga ataaacaata gcatatacta
    11341 aaacatccac cttatattaa ctaaatcata tctaaataaa atgcacttac ctataaacat
    11401 tataaaatca atacaaaaat gaggtgaaac aaaatttatg ataaagaaat taaacaataa
    11461 agacataaat aaaatcatgg aaatatggga aaaaagtaca atcaaagcac atgactttat
    11521 aagtaaagaa tactggcaaa ataactacaa tactgttaaa aacgaatata tacctatatc
    11581 agatacattt gtatatgatg atggagatga aataaaagga tttataagca taatagataa
    11641 aagctttata ggagctttat ttatagaacc caaataccaa aatctaggta tcggaggtaa
    11701 acttttagat tatgcaacta aaaaatataa aagtctaagt ttagcagtat ataaagataa
    11761 taaaaaagca gttgtgtttt ataataaaaa aggttttaat atagtaaaag aacaagtaaa
    11821 tgaagattca ggatttaaag agtacataat ggaatatagt aaataatatg attacattct
    11881 attaaaagaa taattaattt attttctatt tgatttttat aagagtaatt tttaaaatag
    11941 actatatgaa gataaagata tttattatag atgtaagtta atcagaattg atatagctac
    12001 aaaagaggaa aataaaattg tatggtgtat ttcagattta acaaatttac tatttaaaga
    12061 taataagttt gatataatat taaagatact ttcacctata aactataggg tatttgaaag
    12121 aattttgaat aattatggaa tgctaataaa ggtttgttaa aatttaataa ttattagatt
    12181 atagaaaatt gccatgaaat tagttcagca agaactagtt tcatagcaat tttaattctt
    12241 ataagtgtat ttatttttac agatttaaaa aacttataca gtatctaata atgtaatctt
    12301 attactttag cctataaata tttaaatctt aaaatgttct ctttgagata agtttatttc
    12361 agagtcacaa tttccacaac agtaagatgt tgagacatct ttaaattttt cagtattctc
    12421 atcaaagttc atttttttct ttgcaataat tcctacctta gttaaattag aaccacaatt
    12481 attacagttt tgaattttac ataggttata tagtttatta agtgtaatat cttctaataa
    12541 ttcatcacaa ttacaacatc tgatagaaga aacttcacta gacatttcaa taggttcagc
    12601 ttctttagaa gaaaatttat atgttatgtc atttgatact ttataacctg tatcaaatat
    12661 attagaatta caacttggac ataagtactt tggaagatat atatcttttt tttcaaaagt
    12721 atcactacta atattacagt ttaattcatc taaaattggt ctttcatcta ggttattacc
    12781 tttatttaag cttagaagat aattataaat ttttttatct tcattctgtt ttgatgtatc
    12841 taacaaatgc tccattatat tagtgtattt tttgtttttt gaaggcatgt ttaaatccag
    12901 ataattgttt tcaatagcaa agcatactat attgaaatag ttatatttaa ctgcaaaaaa
    12961 taataacatt tcatcaaata ctttatctat tattttgtca gaaatactta tccaaaaatc
    13021 tggtgactct aagtaaactt tcctactaag ttcatctaac tcttcatcta tattatttat
    13081 attattttca ttaactttca tatcatcact tccttttaaa tatcttgtgt ataaactatt
    13141 tgttactatt ttttatataa aataaaatct tatataaaaa tatatcatat tataatttac
    13201 aagtatagtc taaatagagc tgtttttaag caaagtttta taaatgacta ttaaaatata
    13261 aagtaacagt ataaagttta cttataaatt aaacgtaaac ctaaatactt gtaatggttg
    13321 acaaaaaaac gtctttattt tatactaaaa caaagcttta atagaggctt tgtatatgta
    13381 catataaact aaatgaaata agacatatga agggaattat aaaaatgaaa aatactaaag
    13441 taaatattca agatttaact aaaatgtcaa tatgtgtagc attattgtgt atatcatctt
    13501 acatatatat accattgcca tttactccag caggagtaac agcacaaaca ataatgataa
    13561 atttgatagc tttgatttta acaccaagac aagcatttag tacagttggt gtatatataa
    13621 tgataggttt aattgggatt ccagtatttg gtggaggtac atctggtatt ggaaagttgt
    13681 taagcccaac aggtggtttt tattttgggt ttttgtttgc agcactaatt attagtttat
    13741 taaaaggacg aaataatgat attaagagat atatactaat aacaattttt gttggaatgc
    13801 ctattatata ttttatggga acagtattta tgtgctattt taatcaaatg acagtagaag
    13861 cagcactttt tgcagcagta gtaccatttt taattggaga tacaataaag tcggttattg
    13921 catctttttt aggcactaaa ctgaacaatg tactcagaag aaatctatga taaatctata
    13981 atataaggaa gggtgtaatg tatgaaggaa gtttttatat taggaggatt gagaagtcat
    14041 ataggtctaa aaaatggaat ttttcaattt gttcaacctg aattactagg agcaagtgtt
    14101 ttaaaagatt taattaaaaa gtatgaaatt gacaaaattg atgagattat atgtggaaat
    14161 gcagttggga caggagggaa tatagccaga ttaatgactt taacagcagg agtgtcaaat
    14221 gaagtacctg cttttactgt ggatatgcag tgtgcttctg ctatgatgag tatagacata
    14281 gctttttcaa aggttaaatc tggtcagtgt aatttaatta ttgcaggtgg ttttgaaagt
    14341 agttctctac aaccaatgcg cacatatcat aagaatgata agcgatataa tacaaataat
    14401 ccaaattata cagttgctca attttctcct gatgataata gtcaaaatag tatgctcgaa
    14461 ggggcggaaa gagtagcaga gttatatcaa attgagagag cagacttgga tttttgggta
    14521 aaggaaagtc ataaaagagc aaaagaagca agagaagaaa aaatacttga ggatattata
    14581 tctcctataa ataatagtac ctatgatgaa ggtatacgtg ataagatgag tcaaaggtta
    14641 ttagatagga tgccaccaat tttaggaaaa gaaactatta caaatgcagc aaattcatgt
    14701 cttattaatg atggagcttc atttattata atatgttcta agaagtattt agaacatgta
    14761 aaaaagaagc ctaaaacaaa aattattaat acatgcacaa taggaacaga tgcaacctta
    14821 agtccaacat cagcgataca agcaatggat aaacttttag agatagaaag ccttaattat
    14881 ttagatgtat ctgctgttga atttaatgaa gcatttgcag ttatagatgt gttgtttcaa
    14941 agaaagtatc cagagttaat agagagatat aatatatttg gtggagcttt agcgtatgga
    15001 catccatatg gtgcttcagg tgcaattatt acattacatc ttttaaaagc attagaaaaa
    15061 actaagggta gatatggaat ttgctctata gctgcagctg gtggtctagg ttcagcttta
    15121 ctgcttgaaa gggtgtagat tatgaactat tatgaacttt taagaataaa aaaagagagg
    15181 cataaaaata aaaatttttt aattatagat ggagaaaaat atacttatga aaatatatta
    15241 agttatagtg agcaactagg aaaaca
//
LOCUS       DACTGW010000078        15128 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.078, whole genome shotgun sequence.
ACCESSION   DACTGW010000078 DACTGW010000000
VERSION     DACTGW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.078"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            223..1836
                     /gene="ggt"
                     /locus_tag="I9573_001898"
     CDS             223..1836
                     /gene="ggt"
                     /locus_tag="I9573_001898"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAT4796521.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(1969..2820)
                     /locus_tag="I9573_001899"
     CDS             complement(1969..2820)
                     /locus_tag="I9573_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAT4796522.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            3446..4339
                     /locus_tag="I9573_001900"
     CDS             3446..4339
                     /locus_tag="I9573_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HAT4796523.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     ILKLEEGDSHHHSNCGCGGNK"
     gene            4339..5733
                     /gene="gltA"
                     /locus_tag="I9573_001901"
     CDS             4339..5733
                     /gene="gltA"
                     /locus_tag="I9573_001901"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HAT4796524.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLNINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKTKVNA"
     gene            6099..8318
                     /locus_tag="I9573_001902"
     CDS             6099..8318
                     /locus_tag="I9573_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT4796525.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            8465..9211
                     /locus_tag="I9573_001903"
     CDS             8465..9211
                     /locus_tag="I9573_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796526.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PTILLADEPTGNLDSKSTDDVLKLFTRINKEQNVTTLMVTHEVYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            9211..11091
                     /locus_tag="I9573_001904"
     CDS             9211..11091
                     /locus_tag="I9573_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4796527.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELTILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEIYFPVKAILLTIVS
                     FMILFLITGPMSLKLLRKKSILELLVGTKKPKKEIKSSRLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYDEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFRLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            11210..12601
                     /locus_tag="I9573_001905"
     CDS             11210..12601
                     /locus_tag="I9573_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT4796528.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            12708..13748
                     /locus_tag="I9573_001906"
     CDS             12708..13748
                     /locus_tag="I9573_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HAT4796529.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            14237..14890
                     /locus_tag="I9573_001907"
     CDS             14237..14890
                     /locus_tag="I9573_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAT4796530.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIHGIIDGMKF"
BASE COUNT         5980 a         1561 c         2617 g         4970 t
ORIGIN      
        1 tatttttaag tcaaaagaat tagatatatg ttttgaataa aataacttat ttaagtgcag
       61 atagaaaata ctagaattga gtttaagata tttatttaaa gataaaatta aagttaggaa
      121 aaagtctata ttttatatca ttttgtatca gttacattac ttgatgttat ataataaatc
      181 taagcagaat atcaaataaa aaatgacgag gagctgaaat ttatgaagtt taatgcctac
      241 caacataaat actcatcaat aagaaatgtc gtgtatgcta aaaatggagc agttgcaaca
      301 tcaacaccat tagcatctca agctggactt gaaatactaa aaaagggtgg aaatgctgtt
      361 gatgcagcag ttgcaacagc cgccacatta gcagtagtag aacctacaag caatgggatt
      421 ggaggagatg catatgccct tgtttggata gaggaagaaa aacgattgta tggtttaaat
      481 tctagtggct ttgctccaga aaatatggaa ttaaaaaatt ataataatat gaaagaaatg
      541 ccaaaatatg ggtttggagc agtaactgta cctggaatac ccgcagcttg gtcagaatta
      601 aataaaaaat atggtaaact tagcttaatg gaatgcttat ctccagcgat aaactatgca
      661 agagagggat acgtagtatc tcctaatgta gctaaggtat ggaaaaaatc atatgaactc
      721 tatgaaaaag aacttattgg agaagagttc aaaccatggt ttgatacatt ttctaaagat
      781 ggtaaagctc cagaggctgg agatatattt atatgtgaag aacaggcaag tacattagaa
      841 gagatagcaa atactcaggc tgaaagcttt tatagaggaa gacttgctga taaaatagat
      901 gaatattcta aaaaatttaa tggggctata agaaagagtg acttagagtg tttttatcca
      961 acatgggtag aacctataag tacagaatat aagggatata aaatatttga aatcccacca
     1021 aatggtcatg gaataacagt tttaatggca cttaacatat taaaagaatt ggaattagaa
     1081 ggaaatatag aaaatgtaga agacatacat aaaattatag agagtttaaa attagctttt
     1141 gcagattcta aaacatatgt aactgatata gagcatatga aggtaaagat tcaggaatta
     1201 ctaagccaag agtatgccaa aaaaagaagt ttattaattg acaataaaga ggctttgtat
     1261 ccaactgcag gagagccata ttgtggtggt acagtatacc tttgtactgc agataaggat
     1321 ggaaatatgg tttcatatat acagagcaat tacataaatt ttggttcagg aattgtgata
     1381 ccaagaactg ggattgcttt acatagtaga ggaaataatt ttaatttaga ccctaaacac
     1441 cacaatgtag taaaaccatt taaaaaacct tatcatacta taatacctgg atttttaggt
     1501 aaagaagata aagcaatagg tccatttggt gtaatgggag cttttatgca accacaagga
     1561 catattcaag tgttaacaaa tatgattgat tttggattaa atccacaaga ggcgctagat
     1621 gctccaagat ggcagtggat aaaaggaaag gaaatagaag ttgaaccaga gatgccaaag
     1681 catataatag attcattaat tgaaaagggt catgaaataa aagtaattca tgatactgtc
     1741 gacatgggac gaggtcaaat aatatttaaa acagaacagg aaagttatat ttgtgggacg
     1801 gaatcaagat gtgatggtca tattgcagtt tattaatatt atcaactaaa atatatataa
     1861 taaatttaaa gagtatatga aataattagt aaaggcgaaa gtctacaatg actgacgcct
     1921 ttacaaaatt taagctatca tatgctgatt ttatatagat tatgagcctt attcttgacc
     1981 agttttgatt tcattgtcat cataactaat ccagtcacta aagctaccaa tataaatctt
     2041 gtgaggtata tccagttctc taagagcaag actgttaaca caagcagata tccctgaacc
     2101 acaagataaa attacttcat cataattatt taaatcttta aagaattttt ttaaaaattc
     2161 tatatcttta agtgagccat tttcaaagtc actttttatt aaatccttac aaaaataatt
     2221 ttttgcactt ggtatatgac cagcttttga gtaagcaggt tcaactaaac cctggtatct
     2281 ctcattagca cggcaatcta ttattacagt atcttttttg tataatttag acttaacata
     2341 ttccattgga acaactaaat cattgtttat atttggtcta atttccttac ctgtacaact
     2401 tggtatattc actttctctt ccaattctcc accttccttt acgaaagatg ttattccccc
     2461 atctagtaca tagacatttt taagccccaa atgttttaat tggaaaaata atctgcaagc
     2521 tccatttaaa tctccatcat cataagttac aatcgtagtg tcattatcaa cacccatttt
     2581 ttctaatttt tcttttagta ttaaagggtc ttgtaaagga tttttaccac catgttcttt
     2641 tgtaggagat gataaatcct tgtctatatc taagataaaa ctgcctttga tatgtccttt
     2701 tttataagaa tcaataccat aagttctatt gataagatca aatctgcaat ctattacaac
     2761 tagattatca tttttttcta gttttgatat aagttctttt gctgaaataa tgtttttcaa
     2821 agaatgtacc tcctatgttt atattatctt tattttaatt tatattaatt attaacttta
     2881 taaactaata ttattataat ttattacata agtaaataaa tctttagaaa ttgtaaaaat
     2941 tttatataaa aattcaaata attaatgaat attgaaaatt ttagatatat aataaaataa
     3001 gagtatattt tatataatat gaaagctggt attgcaatag attaaagcct tatacacgtt
     3061 tagtttttaa agaaattagt gttattatga aaacctaagt taatttatta aataaataat
     3121 tgaataaaaa aattttatta tgtatttata aaatttttgt tacaaatttg gcataatata
     3181 gtatacttta agtgtgtatt aggttaaatc tataacaaat tagacgtatg atatagactt
     3241 agtgtttatt ggcatttaca agaaatttgt taatatcaac tatttgtagt ttgggcagtt
     3301 ataattacta taggggggat gtccaataca aaacaatcaa ccaagaacta atattatatt
     3361 ataaagagtt atagttttgt gtgtttatgt ttttaattaa atttaaagtg ggaaaaatat
     3421 atttgttaga atggagaata gcgagatgag taataaaata gttagtaaga gacaattaac
     3481 agatagtatt tatttaatgg aaatagaggc acctagagtt gcaaaatctt ctcaaccagg
     3541 tcaatttata ataattaaaa atgatgaaaa gggagagagg attcctctta caatagctga
     3601 ctatgataga gaaaaaggaa cagtaactat tgtattccaa acagttggtg cttcaactaa
     3661 aaagttagca atgtttgaag aaaacgattt tgtaatggat tttgtagggc cattaggaca
     3721 agcaagtgaa tttatacatg aagatataga agaattaaga aataaaaaga tattatttgt
     3781 agctggtgga gttggttctg ctccagttta tccacaagtt aaatggttta aagagcatgg
     3841 attagatgta gatgtaataa ttggtgcaag aactaaagaa ttaattatat tagaagatga
     3901 tatgaaaaaa gtagctaaaa atgtatatgt atcaacagat gatggtacat atggatttaa
     3961 tggaagagtt acagacctat taaaagattt agttgataat caaggtaaaa aatatgacca
     4021 agcaatagta attggaccaa tgataatgat gaaatttatg tgccaattaa ctaaagaact
     4081 aaatatacct actatagtaa gtttaaatac aataatgata gatggaacag gtatgtgtgg
     4141 tggttgtaga gttagtgttg gaaatgaaac taagtttgca tgtgtagatg gtcctgaatt
     4201 tgatggtcat ttagttgatt ttgatcaagc aatgagaaga cagtctatgt acaagactca
     4261 agagggaaga gctattctaa aacttgaaga aggcgatagt catcatcata gtaattgtgg
     4321 atgtggaggt aacaaataat ggatgctaaa aaagtaaaag taccagttag agaacaagaa
     4381 cctgctgtga gagcaacaaa ttttgatgaa gtatgcttag ggtataataa agaagaagct
     4441 atggcagaag ctaatagatg tttagcttgt aaaaaaccaa aatgtgtggg tggatgtcca
     4501 gtaggaatcg acataccagg gtttataaca aaaattaaag aagatgatat agaaggtgct
     4561 gctaaagtaa tagctaagag cagttcatta ccagctgttt gtggtagagt atgcccacaa
     4621 gaaagtcaat gtgaaggtgt atgtatactt ggaataaaat cagatgctgt atcaataggt
     4681 aaattagaaa gatttgtagc agattggtca aaagaaaatg acataaattt atctgataca
     4741 gagcctaaga aaaatcaaaa agtagcagta ataggaagtg gtccagcagg tctagcttgt
     4801 gctggtgatt tagctaaaaa aggctatgat gtaacaatct ttgaagcaat gcatgagcca
     4861 ggtggagttt taacttacgg aatacctgag tttagacttc caaaacaagc tgttgttcaa
     4921 cctgaaatag acaatataag aaaattaggt gtaaaaatag aaactaatgt aattgttggt
     4981 aaaacaatta cagtagatga acttatagaa gatgaaggat ttgaagctat attcataggt
     5041 tcaggagctg gtcttccaat gtttatgaat ataccagggg aaaatgcaaa tggagtgttt
     5101 tctgcaaatg agtttttaac tagagtaaac ttaatgaagg cttatagaga tgactatgat
     5161 acacctatta gttctggtaa aaaagttgct gtagtaggtg gtggaaatgt tgctatggac
     5221 gctgctagaa ctgcattaag attaggttca gaatcatata tagtttatag aagaagtgaa
     5281 aaagagcttc cagcaagagc tgaagaagtt catcatgcaa aagaagaagg cattatattt
     5341 aatacattaa caaatcctaa ggaaatttta gtagatgaaa atggatatgt taaaggtatg
     5401 gtttgtataa gaatggaatt aggagagcca gatgattctg gaagaagaag acctatagaa
     5461 atagaaggtt ctgaatttgt acttgatgta gacacagtta taatgtcact tggaacaagc
     5521 ccaaatccac taatatcttc tacaacaaag agtcttaata taaataagaa gagatgctta
     5581 ataacagatg aaaatggtca gacttctaaa gagggtgtat ttgcaggtgg agatgctgtt
     5641 actggagcag caacagtaat atctgcaatg ggtgctggta aaacagcagc agcatcaata
     5701 gatgaatatt taaaaacaaa agttaatgct taatttgtaa attaaagaga gggtgtctca
     5761 aaatgaactt tttagttcat gaggcacttt ttttgtacaa aatcaaatat gttttaatca
     5821 tttcaacaat gaaaatttca ttcctgtaga aagtctaact tttaagtctt agtacaatct
     5881 ctattttgat gtaggcactt ttttagatgg tttaataatt tatcagttat tattgtaata
     5941 tttgatacta agtaacataa ctttgtctaa ctatgataaa atagttatac aataaaggtc
     6001 ttgtaaggag tgaataatgt gtggctaata aaatcagatt tgtcaaatct aacattaaat
     6061 aatagaaaaa taaattgtgt aattaatggt attttaatat gaaaaaaaga gaaaaagtat
     6121 ttgatgggct agtttttaag catatattaa tcttattaat actattagtt ttgattgatt
     6181 taacaagtac catcatatat gcaagtaact ctatggaaat tacttctaga aatatgattg
     6241 aggcatcaaa aagagaactt gaaaactatt tagaagtaaa catatcttta ttaaaagctc
     6301 ttagtcaaga tgatagattt tctaatggtg aaacatcact aattgaaaag ggaaaattat
     6361 taaggccata tcaaaaggaa tataatcttt ttatgattgg tataacagat acaaaaggaa
     6421 atgcttcaag tacatatagg gaaaatgtta actcaataaa agataagcct agttttgaga
     6481 aagcaattaa aacaaagcaa gtagtagttt cagatattga agtcagtaat gtaacaggtg
     6541 acaaagtatt tatcatatat gtaccaattg taaaaaataa tgagatgatt gggaccattt
     6601 ttgcttcttt ttattttcaa gatgtaaata atttaataag tagaagcaat tttgatgatt
     6661 ctattaaatt tctaatgata gataaagatt atactattat ttctcatcca aacaaaaaat
     6721 atgtaaatga taaaagtaag atgcttgatt tagaagggaa tattatagga accactaaaa
     6781 gtgaaatctt aaaaaatata aataaaaaat gtcaaggtga ttttttatct tgggataatt
     6841 ggagattata taatgtaaaa tatacgaaca taaagtggac taattggact ttagtatcaa
     6901 aatgtaatat atttaagaac tttaaaaatc taatagttaa ttttatgata aagttgtatt
     6961 tttatatagt aatatttatg atattgtgga aattaagtaa tgctaaatta atagaacaac
     7021 ttaaaaaatt ggcttattat gattctttaa gtggaataaa aaataaagaa aagtttagaa
     7081 aagattctat gtatatttta aagaattatt atcaagataa tttttactta gtacaattgg
     7141 atgtcaataa atttaaatat ataaatgaga tgtttggata tgctgaaggg aataagatat
     7201 taattcatat atcacaggtt ttaaataata atacaaataa atatgaaatt tgtgctagga
     7261 tggataatga ccattttata ttgttgattg cttgtaatac tgaagatgaa cttttaaata
     7321 gattatctaa aataaataaa gaaatttgta acttaaatac tactaactca tctaagtata
     7381 aaattgttat gtcaagcgga atatataaga ttactaaaaa ggatgatatt aaaaagattg
     7441 acctgttaat tgatagagca aatattgcag caaaaagtaa aaaggaaaaa tatgaacatt
     7501 catattcgtt ttttaatgaa gatacaagaa atcgattgta caaggaaaaa cgattggaag
     7561 ataatatgaa taaagcttta gaaaaaggtg aatttatagt atattatcaa cctaagtata
     7621 gtttagatga tgtaaatgaa attgaaggag cagaagcctt gataagatgg aatagtccag
     7681 aatttgggtt tatttcacct atagattttg tacctttatt tgaaaagaat ggttttattg
     7741 taaatattga tatgttcgtg tttgaagaag tatgcaaaac attaaacaag tggataaata
     7801 aagggtatac tcctgtacca atctctgtaa atatgtctag agttcattta tatagggata
     7861 attttataga aaatataact gatttgataa gtaaatacaa tatatctcct gagtttattg
     7921 aacttgaact aacagaaagt gttgtgtttg ataatttaaa tatattgatt gatataatga
     7981 aaaagattaa agagatagga ttccttatat caatggatga ttttggttcg ggatattcat
     8041 ctttaaatct gttaaaagac ctttcttttg atattttaaa attagataga ggatttttaa
     8101 tcgaaacaac agatacaaaa cgtggaaaaa ttattatttc taagattgtg gaaatggcaa
     8161 aagcgattga tattaaagta atctgtgagg gcgtagaaac ttatgaacaa gtagagttct
     8221 tgaaagagat aggttgtgat aaggttcaag gatacttatt tgctaaacct atggtattag
     8281 atgaatttga aaaacactta aactttaaat ttgattaatc attaataatt attagactat
     8341 aatataccct aacaacttac ttacaaatat gtaagttaat tgttagggta tttttatggt
     8401 taaagtagta tattcacgat attatggtat tgtagtaaaa ataaaaaatt atggagaata
     8461 taaaatgaac atattgagta ttagaaatat atcaaaaaca tattgcggaa atattccatt
     8521 taaagcatta gataaggtta gtctaaatat agaaaaggga gaatttgtgt ctgttatggg
     8581 accatctgga agtggtaaat ctactctttt aaatataata tctacagttg atagacagag
     8641 tgaaggtgag gttgtattag atggatatga tgtaagtaaa ttgaaaggag aaaaacttgc
     8701 tgaatttaga aggaaacaac ttggttttgt atttcaggat tttaatttga ttgacacttt
     8761 aactgttggc gaaaatatta tgttgccact tacactagag ggggcaagta tcaaagatat
     8821 gaatatacaa actaaaagta tatctaaatt tttaggaata gataaaattt tagatagaaa
     8881 aacctatgaa atttcaggag gtcaagctca aaggtgtgct attgctagag ctattataaa
     8941 taaacctacc atacttcttg cagatgagcc aactggaaat cttgattcaa aatctactga
     9001 tgatgttttg aaattattta ctaggataaa caaagaacaa aatgtaacaa cacttatggt
     9061 aactcatgaa gtatatagtg caagttattc agatagagta atttttataa aagatggttg
     9121 tatatataca gaaataaaaa aatctgaaag cagtaattct ttctattctg atatactagc
     9181 tgttttgtct caaataggag gtgtaagata gtggatttta gaaagtttgc ttataataat
     9241 atagttagaa atttcagggc atatttagca tattttttaa gttctgtttt ttctataatg
     9301 atattttttg tttttgcagt gtctatgttc catccgatta taacaaaatc tggaatgcag
     9361 agtggttcta ctgcatttat ggctcttatg acttcagaat taacaatatt aggttttagt
     9421 ctacttttta tattgtactc tttaggaaat ttcttgaaat atagattgaa agattttgga
     9481 gtattgatga taattggaat gagcaacaga caactgaaaa aattgatatt aattgaaaat
     9541 ttgataatag gatttttagc agtgatgtta ggaatattac ttggtttatc aatttcaaaa
     9601 ttgtttttat tgtatttaag caaactgttt tatatgaatt taactgaaat ttattttcca
     9661 gtaaaagcaa tactattgac tattgtatca tttatgattt tatttttgat aacaggacca
     9721 atgagcttaa aattattgag aaaaaagagt attttagagc ttttagtagg tacaaaaaag
     9781 cctaaaaaag agataaaatc ttcaaggcta tttggaataa ttggtgtaat atgtcttata
     9841 ttaggatatg ctatgatact atttggcaat aagttaaaaa taaatggagg tatgtataca
     9901 acagcactat taattacttt ggggatattt ttattttttt ctcaatttac tattttgata
     9961 ttaaagtatt taaaaaataa agaaaaattt tataagaaca aagtaaatat gttacttata
    10021 agtaatttga tatataaatt aaaggataat acacgattgt tatttttaat aacaatactt
    10081 ttatcggcta ctttagtagc atttacaaca acttcaacac ttgtaagttc tcaaggagaa
    10141 gcttctaaaa ataatttccc aatggtttac tcatattttt cagagcataa taatgaaaaa
    10201 gaatatgaag aactacaaca aattagagaa gcaatcaaag gatatgatta taaggagtta
    10261 agttttcctg tacttaaata taatagggaa gtcttactaa gtgtaaagaa ttataacaaa
    10321 ttatcaaagg aattgggatt agataaaatt agttcaaagt tagaaaaata tgaagggatt
    10381 ataattccta gtagcaattc acaagaacat ataaattctt taaaacaatt aaaaaattat
    10441 agtataaaaa atttaaatat agaagtaaaa aaatcaaaaa ataaaataat ttttcctaca
    10501 ggactgttta gtaaagttgt agttataagt gatgacttat acgatgaagt aaaaggtgaa
    10561 ttttcaaaaa ttaattttca tggatttgat tataaaaact ggcaaagcag tgcaaagata
    10621 actgaaaatt taaaagaaaa acattttaat ttggatagag aatataacag gtcatatttt
    10681 ttaacattac cagatatgta tttagtagag ttacaacaaa gtaaaattct tcaatttatg
    10741 ggtgttttta taggagtgat attatttata gcagttttta gttttttata cttcagatta
    10801 tatacagacg agatagaaga taatgtaaag tacaaaaaac tatcaaaaat aggtctatct
    10861 tttaaaaata tgaataagat tgttagtata gagataggta cattgttttt tataccgtat
    10921 tttatagcaa ttataaatag cataatttct ttactgtttt taaatcaagt attaaataat
    10981 agttttaggt tagaaaattt gggtatatta ctactgatat catctatata ttcactttat
    11041 ttttacttat taaaaaaaat ttatactaga aaagtttggt tttttgatta aagtggtata
    11101 taatagatat ataaattaat atttaaaaag tagttaatat taggggaggg catttgcacc
    11161 tcccttgtaa tatgtggaaa attgaatatt agaagaagga ggagacttga tggaaaatca
    11221 acaattatta ggaacagaac gaattagtaa attactacta aaatattcta taccagctat
    11281 aataggtatg ttggtaaata gtttatataa tgtagtagat agaattttta ttggaaatat
    11341 accaggggtt ggaccacttg caataacagg tcttggtgta acaatgccaa taatgacaat
    11401 aatacttgca tttgggatgt tgataggtat aggaactact actaccatat caataaaact
    11461 tgggcaaggt aaagtagaag aggcaagaaa acttatagga aatgcaatga cgttatctgt
    11521 aataacagga attatcataa tgatacttgg aatattattt gcaaataaaa tacttacatt
    11581 gtttggagca agtgaaaata ctttaatata tgcaaaatca tatataaata ttatattatt
    11641 aggtacagta gtaaatcttc tttccttttc tttaaaccac tcaataagag ctgatggtag
    11701 tcctaaaatt tcagcaggaa taatgatagt aggatgttta actaatatag ttttagactg
    11761 gatattaata tttggattca atttgggaat acaaggagct gcaattgcta cagtaacttc
    11821 tcaggctctt acagctattt taactatagg gtattatata agtggaaaat caaacttacg
    11881 attcagtaaa tctaatctaa aattagataa aaagctaata aaagcagtgt ttgcaatagg
    11941 tatgtcgcca tttgcgatgc aacttgcagc aagtttagtt caagtaatat caaatattgc
    12001 acttaaaact catggtggag atttagctat aggggctatg gctactattt cttccattgc
    12061 aatggtattt ttgatgccta tctttggaat aaatcaagga gcgcaaccta ttataggttt
    12121 caattatgga gctgaaaaat atgatagggt taaaaaagca tatttaggtt cattagtagt
    12181 agctactata attttatgta tgggtatggt agtagttatg cttttcccag aagctattat
    12241 aggtatattt aataaagacc ctgaacttat gaatatatct gttaatggat taagaatata
    12301 cttacttatg ttgccaattg taggcttatc agttacagga acaaacttta tacaatctat
    12361 aggaaaagca aaaatggcta tgttattgag ccttttaaga caagtaattt tattgatacc
    12421 agcagtatta attcttccta cattcttagg tttacagggt gtatggacag ctcaaccagt
    12481 gtctgatttt atagctacag taattacagg tatagttgta tttagagaat taaaaagata
    12541 tactcctaaa actgaaaaat taaatgaaaa tgaaagacta aatgagataa ctactgagta
    12601 aaaatataaa ttcagataat tgagcaagaa attgcaaaaa atacgattat atgataaaat
    12661 aaaaccgctg tcaaggataa gagaaattct aattatggag tgattttatg gaatttacaa
    12721 agaaaagcaa attaaaaaat atgtatgtaa acatgaatgt agctagaatg ttgtctaaga
    12781 taaatgagta taagggtagg caactgcttt acaaaaaaca accaaaagaa attttagaaa
    12841 acctagaaaa aaaatcttta gtagattgtt ctgaatctac atacattgga aatcaaagag
    12901 atagtagtaa ttttaatcta gaaaagttga taagtaatga agttacacct agaagtagag
    12961 aggaattgag tattgttgaa tacagagatg tggtaaaaac tattaacagt gcttatgaga
    13021 gtattcccat cagttcacaa acgattcttg agcttcatgg gtacttatat aaattttcat
    13081 ctactagagg aggaagttat aagtcagata atgattttat tgaacacaat ttaaaaccta
    13141 gcgaatttat aagtattgat tcaagtgtca ataaagttga aaaagctgta gaagaaatat
    13201 gtgaagctta taatacattg atagaggaag atgaaattga tatattaata ttaatttctg
    13261 catttgtgtt agatttcata ttgatacatc cattcaaaga aggtaatata aaaatggcga
    13321 gagtacttat cctattgtta ctaaataaaa atgggtatga agttggaaga tatataagtt
    13381 taggaaagat atttgatgat agttcatatg aatattatag taatttaaat tatttgaaag
    13441 cctcaatagg tagtgaaaaa gcagatatga atgcatggat tgagtatttt ttagagacga
    13501 tattaactgc atatgagaag ctggatgata gtttaaatat atctgataag aaaagacaaa
    13561 caaaaactag tagaatagaa aaaataataa attctaccct tggatatttt acaaaagaag
    13621 atataagaga tttatgtcct gacattccag aacctactat aaatagagtt tttaataatc
    13681 taagaaaaca agataagata gaagttgttg caagaggaag aagtgcaaaa tggaagaaaa
    13741 aatattaaaa aatgtttaaa taactaaaat ggtatagtgt tttacactat accattttta
    13801 atgtcattag catgagcaat aatttaacaa cttaagcaat atcaatttta tttattgatt
    13861 atttttgaac tgaaaaatgg ggttatatgg tatactacaa taataattaa ttttggatga
    13921 gaatatataa cgttacagaa tatcttagga gtatatgtat aaaaaatata ataaaagtgt
    13981 gaggtttagt aattaacaaa ttatgtggct tcacactttt ttataaaata aaaatactta
    14041 gcagaagaat attttagttg atatgtttat aagatagtac ttgtttgggt aaaagtataa
    14101 atagtaaaaa agtagaagta atgtgtataa aaacataagt tacattctgt aataatagtt
    14161 attttataat tcaacaaaag aatagaatgg gtatatttgt gaaataataa tataaaataa
    14221 tcttaggagt gatcttatga agaaaaaatt attatatata aatgtaaatt caaaacctga
    14281 agacttatct tcaagtaaaa ctgtagcaag aaaatttata aataaattta tggaaaaaaa
    14341 caaagatttt gaggttgaag agatagacct ttataaagaa catataccga gacttgagta
    14401 tcagtacttt gaaaaaagaa atagcatagt tagtgaagaa aatgctaaaa atttagacga
    14461 taaggataga aaagagctta caaagattag aaatctttgt gaccaatttg tgagtgcaag
    14521 tgtttatgta atagcagcac ctatgtggag tttatctttt ccagcaccac ttaaagagta
    14581 tatagattgt ataattcaag atggaaaaac tatatctttt gaaggcaatg ataaaccaca
    14641 aggaatttta aatgatattg atagaagtat ggtatatata caatcttctg gtggacacat
    14701 accatgggtc ttgaagccag ttatgaataa agggttaaat tatgtggaaa gtattatgaa
    14761 atttatagga attaaaaaat ttgatgaatt gttagtagat ggaactggaa ctagtgaaga
    14821 agaaagacaa gctgcaatag aaaaagcatc tgaaaaaata catggtataa tagatggtat
    14881 gaaattttaa ttataatatt ttattaaaaa tataaaggaa aaacctcttt taatgtagaa
    14941 acttatattg aaggaggcga gaatcatgcg agaagaaaaa agtaatgaaa agtatgattg
    15001 ttattggtgt aatcaagaaa ataatttctg tgtagaaata aaagataata tagtaatgat
    15061 agatgatggc actggtacgt taaaacaagc cgttttcata gggtataaac aaatccaaat
    15121 taatttaa
//
LOCUS       DACTGW010000079        15063 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.079, whole genome shotgun sequence.
ACCESSION   DACTGW010000079 DACTGW010000000
VERSION     DACTGW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15063
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.079"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            143..1078
                     /locus_tag="I9573_001908"
     CDS             143..1078
                     /locus_tag="I9573_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAT4796531.1"
                     /translation="MDKFKYAFDDKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(1349..2293)
                     /locus_tag="I9573_001909"
     CDS             complement(1349..2293)
                     /locus_tag="I9573_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAT4796532.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            2609..4039
                     /locus_tag="I9573_001910"
     CDS             2609..4039
                     /locus_tag="I9573_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HAT4796533.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNADLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            4239..4823
                     /locus_tag="I9573_001911"
     CDS             4239..4823
                     /locus_tag="I9573_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HAT4796534.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(4927..5706)
                     /locus_tag="I9573_001912"
     CDS             complement(4927..5706)
                     /locus_tag="I9573_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAT4796535.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(5776..7032)
                     /locus_tag="I9573_001913"
     CDS             complement(5776..7032)
                     /locus_tag="I9573_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAT4796536.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            7290..8189
                     /locus_tag="I9573_001914"
     CDS             7290..8189
                     /locus_tag="I9573_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAT4796537.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            8213..9661
                     /locus_tag="I9573_001915"
     CDS             8213..9661
                     /locus_tag="I9573_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAT4796538.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            9744..10526
                     /locus_tag="I9573_001916"
     CDS             9744..10526
                     /locus_tag="I9573_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796539.1"
                     /translation="MSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSNPNKKGNIK
                     SQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGKSMPTDEPI
                     SFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEKIKLIDDYD
                     AVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDVTPKISTVY
                     ADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            10552..12471
                     /locus_tag="I9573_001917"
     CDS             10552..12471
                     /locus_tag="I9573_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAT4796540.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVKRGAQ
                     ARSTKQKARLQRFDELMNRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(12713..13060)
                     /locus_tag="I9573_001918"
     CDS             complement(12713..13060)
                     /locus_tag="I9573_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HAT4796541.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(13047..14975)
                     /locus_tag="I9573_001919"
     CDS             complement(13047..14975)
                     /locus_tag="I9573_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HAT4796542.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTTLPIVLSKVKNNEQE"
BASE COUNT         5694 a         2199 c         2057 g         5113 t
ORIGIN      
        1 gaaatacagc gagaaaatat taattatttt ctcgctgtat ttttatatta cttaaaatag
       61 aatttgttta agttatctat ttgtggtaat atgttttttg tacaagctca gcgtacaatt
      121 ttattagaaa taggtgattt ttttggataa atttaaatat gcttttgatg ataaaagata
      181 tcatacttgg aattactatc ttagaaatac ttttggagaa aaagttttta aggtatctat
      241 aaatgcagga tttacctgcc caaacattga tggaagtctt ggttatggcg gatgtaccta
      301 ctgtagcaaa gaaggctcag gtgattttgc tggaaaccct aaagataatc ttatcagtca
      361 attttataat ataaaagaaa tgatgttaaa aaaatggcct catgcaaagt atattggcta
      421 ctttcaagcc tatacaaata cgtatgctcc tcttgaagtt ttaaaagaaa aatatgagac
      481 tatattagaa cttgaagatg tcataggtct ttcaatatcc acaagacctg actgtttgcc
      541 agatgatgtt gtagaatact tatctgaact aaataaaaga accaatttat gggttgaact
      601 tggacttcaa acaattcatg attctacatc taaaattata aatagaggtc atgattataa
      661 aacatttgtt gaaggtgttg aaaaattaaa gtctaaaaat ataaaggttg tcgtacacat
      721 tataaatggt cttcctggtg aagattataa tatgatgatg gaaacagcaa aggcagttgg
      781 taaaatgggt gtagacggta taaaaatcca tctattgcat gttataaaag atactcctat
      841 ggaaaagatg ttacaaaatg gtatgttaac tcttatggaa caagatgaat atatcaatct
      901 tgtctgtgac caattagaaa tacttcctga aactatgata atccatagac tcactggtga
      961 tggtaagagg gatgaactag taggtcctat atggagccta aaaaaatggg aaattttaaa
     1021 tcaaattgat gatactttaa aggctagaaa ttcttatcag ggctgtaagt ttgtttaata
     1081 caaaataata actatagaaa tggttatata aatatagtcc tttctatagt tatttagtat
     1141 ttacaaaaca aaatattaat ttgtacattt atacaatata taataaaaaa tagatagtct
     1201 aaactgtatc tacttatatt ttatttgtac tgtccacttt attctataaa atttataaat
     1261 agttattttt taataccaaa tataatttgg tataattttt gttggattct aaaatgtata
     1321 ccaataaatc ctttttaagt atataattct aaatactttc agttttttca atctgcttta
     1381 acttgttgga tttataaaat attcttattt catcataaga aagattaagt gctactactg
     1441 cagatattaa catcaacatc actccaaata acttccctaa ggaaaaactt tctcctaata
     1501 tagtgcaacc aattattact gaaccaacta actctactga tgccacaaca cctgcaactg
     1561 acaactctac acctttagat atacctgctg cataaaaaat atatgcaagt gcagctggaa
     1621 caatgcctaa cattagcata caagataaaa catctaaatc cttagcataa tttaagattt
     1681 cccaaggtct cgaagaagga atcataaata tagctccaaa taagaaactg tatactaata
     1741 ttgttgaact tgaaaattcc tttaaagcat ttttactaat agttggcata agtgcatatg
     1801 ctattgctga taatacccca agtaaaagtc caaaagcgtt taaaccttga aaatccaaac
     1861 gtccaccagt cactgccata attgcaccaa taaaacaaag aataagtgaa aataatttgc
     1921 ttctagtaat attttcttta taacatactt ttgaaaatat tgctaaaaac agtggtgatg
     1981 tatatagtaa aactgctgca attgatacac ctgcaatgtc aattgcttta aaataacata
     2041 aattaaacat tgcctgacat ataattccta ttattactga atatataatt ccttttttgc
     2101 ttatctttaa gatttgtggt gtccttatag atgaatatac gcctaaaact ataaatccaa
     2161 aaaacaatct tgtaaatgct acttgttctg atgtcagtcc atttcccatt aaaaactttc
     2221 caaataatcc aagtgtagcc cataataacc ctgctattgc tataaataca taccctttac
     2281 ttttttctcc cattttaaat tcccttctat ttttatttag tcaatttaca ctcttctttt
     2341 taagcaaaaa ttatgccatc ttaaaccaat atatatcgac tctaaaaccc tagtctttcc
     2401 aataatacta ataagttttc aattttctta tatttatttt tcgcttactt taagttgatt
     2461 tagacttatt ttaattgtaa aatgagttat atataactta tttaaagtta aaatagactg
     2521 aaacttatat aaatatcaaa ttttaagtat attaatcaat taaacctatt ttaatatatc
     2581 attaaaaatt taggaggaaa atataaaaat gaatttagat ttggaatttt atcaaaaaat
     2641 attagaagct tcccatgatg aaatatgtgt ttctgatgat aaaggaataa taatatattg
     2701 taataaagca tttgaagaaa attatggtct aaaaaaagaa gatattttag gaaaaaatgt
     2761 ttctttttta gaagactctg gatattcaac aaaaagcccc ataccaattg tattaaaaac
     2821 aaagactaaa ttttcactgg aacaagatac tcaaacaggg aaaaagctta tcataactgc
     2881 tacccctata tttgatgaaa atggaaactt agaatttact gttgaaaatt gtagagatat
     2941 aactgaactt aataatataa aaaataaact tgaagacaca aaaaagcaag taaaaaaata
     3001 taaatctgag gtagaaacac tatacagaac tgcacttaga attgaagata ctgtaattat
     3061 ggacggaata gtgatgagac caatcataaa cacagtaaat catgtttcta aaacggatgt
     3121 aagtgtactt ttattgggtg aatctggaac aggaaaaagc tcacttgcta gatatataca
     3181 ccacaacagc aatagatcta atggcccatt tataacaata aattgtgcaa ctatatctcc
     3241 acaacttctt gaatctgaac tttttggata tacttcaggt gcttttacag gtgcaagtac
     3301 taaaggtaaa gttgggctcg tagaattagc taatggagga actctttttt tagatgaaat
     3361 aggagatata ccacaaaatc tacaggcaaa atttttgcaa ttaatacagg atagaacttt
     3421 tactccagtt gggagcttaa aaaataaaaa ggttgatata agaattatat ctgctacaaa
     3481 tgctgattta gtttcaaaag taaaagaaaa aaagtttaga gaagatttat actatagatt
     3541 aaatgttata gaaataaaat tgcctcctct tagagaaaga agggataatc tagtggaaat
     3601 tataaaatat tattttaata ggtactcaag tgacttcaac ctaaataaaa ctatttctaa
     3661 agaagctatg gatgctattg caaattatag attcccaggc aatataagag aattacaaaa
     3721 tataattcaa aaaatcttgc ttacttgtac agataatcat ataacaatag atgacttacc
     3781 aaacattctt acaaaaaata tacatattac caataatgga aataaaacac atatatccca
     3841 aattaataag gttattatat ctgactcaaa atctactaat tataaaaata aaaattttga
     3901 caccttaatt aaagagtatg aaaaaaatat aattttggat gcttatgaaa aatttggtag
     3961 ttcttataaa gtagccaaac accttgaaat ctcacagtct aaagccaata ggttaattag
     4021 aaagtatact aatacttaat tttaaatttg ataaataaaa acttaacact atttacatat
     4081 tgtaaatttg gccatttact gttattatga atatggttga aattatttaa ataaatactg
     4141 ttgacttagt ttcatatatt taccattaaa cttttatcat aaatatcata tcataataat
     4201 atatttaata agtctaataa actatacgag gtgtaaatat gttaatgaat acccatttta
     4261 ttatggcaaa atcaatactt gataatatag atgaaaataa aacattcttt ataagcgaaa
     4321 aaaattttat atatggcaat ataaagcctg atgctttttc taaatataaa ttaaaaaaac
     4381 attatttaga tgagtcattt aatatgattg tttctaaaat aaactatcta tgcagtctta
     4441 atatagatag tctttctaaa tacttttcag taagtagact tagtcaggaa ctaggagtca
     4501 tatgtcattt tttatgtgat tttttctgtg tagctcacag tgaacgttgg gaatttaagc
     4561 atagcttaaa taaacatgtt acttatgaaa gagaacttgg agcatttgca aaagatgttg
     4621 atttatctaa aattaaatgt ggtactataa atagtagttt tgagaagttt tttactaaac
     4681 tttatagtga ttacaaaaag aaaactgatt atatgaatga tttaaaattt tcagcttata
     4741 cttgtaatag tgtcataaat tatatacttg actctatttt atctaataca ataaaatcat
     4801 atcatatagc aaattgcggt taacttaaga atttttaaat ttatataaac ttataattga
     4861 atataattaa aatgactgct ctatagaata taattttata cttctctagg acagtcattt
     4921 tttatactaa ctatttatct ccattataac atctatggca ctttttacac tatctactac
     4981 ataatttgca ctttgcttta catcttccct tgccttagac attgcaaaac tatatttaaa
     5041 tccttctaac atgcaaatat catttggaga atccccaata gtagcaactt catctggact
     5101 tacatttaac gtttttatta catgctctat acttcctctt tttgacacac ttttaggaac
     5161 tatatttaaa gtatatgttc cagaaaaata tatttctaat acttcactaa actcactttt
     5221 tagataaagt tctattctgg ataactcttc caaattttca gaacatacgc ctatatttcc
     5281 tataagatta tcaaaactac ttcttttttc aaaatctggg tcaactataa agtcactaga
     5341 ataatgtcta aaagcagtgg tatcactacc cacaattaca tcacccttat atttaaaata
     5401 aagttgtgca tcacttgatt caaatctatc aatgactttt tttactaaat tatcatctat
     5461 cgttctttta aaaattacat tataatcttt atcatatata aatgaaccat tttcacatac
     5521 ataatatcca ttcatatcta aactatcttt gataatatta tgtgcttgtt taaaaattct
     5581 acctgtagca acattaaact gtactccctt tttttctatt tcttcaattg ctactatatc
     5641 ttcttttgtt atggttccat tttcatatag agttccatct aaatcacaaa atatatgttt
     5701 tatcataaat taaaagcctc caaaaacaat tttatattat agtaacactt caaatatatc
     5761 caatatcata acaacttaaa gcttttctaa ttgtattctc aattcattaa gtaaatctct
     5821 agtagctctt aatcttatct ttcttctatc cccattaaat atatttttct ttactattgt
     5881 ttttccattt atatatattg cagtataaac tagtcccaca ggcttctctt tggttcctcc
     5941 accaggtcca gctactccag ttgtggatat acctatatta gctttaagtc ctttagcaac
     6001 gccctcagcc atttctatag ctgtttgctc acttactgct ccaaactctt ccaaagtttc
     6061 tctttttact cctaatcttt tcattttaga atcattagaa tatgttacac atccttcaag
     6121 aaatacagaa gatattcctg gatagtttat aagagatgaa gaaaccaaac ctccagtaca
     6181 agattcagcc actgctattg ttagattttt ttctacaagt aacttagaaa cagcatcttc
     6241 taaagcagtc tccccttcag atatggaaat atctccttca gtatatacat atttaccaac
     6301 tctacttttt attttttctt ctactggttt tattaattta aaagcttctc tttcattttc
     6361 agcctttgca gttattctta ttgtaacttc taattctttt gcatataaag caactgttgg
     6421 attagtctgc tcattaatta tatcgagaat ttctaattct agatttgact cacctattcc
     6481 atataatctt aaagtcttag atactagcat ttcatttgtg aattgttgta agtaaggttt
     6541 gacactctca ttaaacatag ctttcatttc tcttggaggt ccaggcaata ctattataaa
     6601 tttaccatct ttttttaaaa ttgcccctgg agctgtacca ttattatttt ttagtactat
     6661 agcatctttt ggaaagtaca cttgtttcat attattttcg gccatttttt ttcctgtttt
     6721 aacaaagaaa gattccaaaa cctctagtga aggtttatgt agttctaatt tctgaccaaa
     6781 gtattctgca gcagtttctt ttgtcatatc atcaccagtt ggcccaagac cacctgtagt
     6841 aattacaaaa tcacttctct ttaagctttc atcaaaacat tctaaaagtc tttgtttgtt
     6901 atctccaaca gtactctgat ggtatacttc tattcccaat gatgcaagtt ctttagctaa
     6961 aaattgagaa tttgtattta ctatatctcc caataatatt tctgttccta cacttattat
     7021 ttcagctttc aaaatttatc actccttgat ttatagccat taagactagt cttaagttca
     7081 taacaataat tttagcaaaa ataataacat aaataaagtt aaaaaatcat ttaaacatat
     7141 ctttaaacta gattattatt ctaaaaccac acataatata aacttcgtat atagtggtac
     7201 tttcatcctc aactgttaat tcatataatt aagtatgtaa ataaaaagtt tactttaaaa
     7261 taataaaaat taaaatagga gtgtataata tgaaaatagc agttataatg ggtggaatat
     7321 catcagagag agaagtttct ttaaattcag gaaaagagat atataataac cttgataaaa
     7381 ataaatatga agttgtaaaa gtaataatag atgataaaaa agatatattt acaaagatac
     7441 cagaagatat agattttgca atacttgctt tacatggaaa atttggtgaa gatggatgta
     7501 ttcaatcaat acttgagact atggatatac catactctgg atgtggacct ctttgcagtg
     7561 ggatgtgtat ggataaaaat ataactaaaa aaatgcttag agattcaaac ctacctactg
     7621 ctccatgggt tttagtaaaa tcagttgatg aaatcgacta tgatgaaata gataatatag
     7681 gttatccagt atttataaaa ccaaactcag gaggttcaag tgtagccaca ttctttattc
     7741 attctaagga tgaagttgaa gaagcagtta gaaaaggtct ggaagttgac gaatttgtaa
     7801 tgattgaaaa atatatacca ggtggagaat atacttcatt catattaaat ggagaagtgt
     7861 tccctacaat atctataaaa tctgattcag gattctttga ttatgaagct aaatactcag
     7921 ttgaaaaagg tgcaaaggaa gaagttgttt acttagatga agaacttcaa aaaagagtaa
     7981 acgaaatttc tgagacttgt tggaaaattt ttaattgcaa agcatatgta agagttgata
     8041 tgattataag tgaaggtatt ccatatgttc ttgaactaaa tactcttcct ggaatgactc
     8101 aaactagttt aattcctaga agtgctgctg ctagaggtat taagtacagt gaactattag
     8161 ataaacttat agaatactct ttaaattaga tgaaataatt tgaggtggta atttgatatt
     8221 ttcaaatcta aaactaaatg ataatgaacc aatttatata caacttaaaa attatataag
     8281 tgatatgata tcaaaaggac taattccaga caatagtaag ctcccttcta caagggagct
     8341 tagtcagctt ttacaagtta gccgaaattc tgtagtttta acttatgaag aattaaaatc
     8401 agaaggatta atctactcta tttcaggaaa aggtacattt gttaaatcaa agaataagtc
     8461 ttcaaatact acatggtcat taaactggga ttgtttagag aatacatact ctaaaaaagc
     8521 caatgaattg gacattataa aaagtgaaat tccttggagc tctgacctga tttcctttaa
     8581 aagtatttct ccagatggag atttattcga catggaagaa ttaaaaaaat cctttttaaa
     8641 tagaatttca ctagaaggac ataaattact caactatgga tatgcacaag gttataagcc
     8701 ccttattgac tatttactag aatatatgac aaacaagggt gctgatataa gtaataaaga
     8761 tatactaata acaaatggat tcacagaagg ctttgatatt attatgtcat cttttaccca
     8821 agaaaaagac tacataatat gtgaaaatcc tactcataat actgctataa aaattatgaa
     8881 atctcataac ctcaatatct taggtgtaga tataaatgaa gatggtttgg acttcaatat
     8941 gctagaagac aatttagtta agtataaaaa taaaataaag tttgcataca ttacaccctc
     9001 ttatcacaat cctacaggaa ttgtaatgac tcctgaaaat aggtacaaat tctataatct
     9061 tatgaaaaaa tacaatatag caattataga agatggattt aatgaagaat tactctacaa
     9121 tagttctcat atatttccta tctgttccct agataatatg aataatgggg tcgtatatat
     9181 tggtagtttt tcaaaaattt tatttcctgg aatgagaatt ggatggatat ttgcagataa
     9241 aaaagtaatc aataaactag aaagtgtaaa aaggtgcaga aacatacatg tatccttttt
     9301 agaccaagga attttatttg attatttaag taatggaggt tttgaaaaat acattaaaaa
     9361 aatacgtaaa ttctatgggg acaaatttaa ctttgcatat aaatgtataa aaaaatatat
     9421 aaaaacagaa tatatcttgg gtgatggagg tttgcatata tttataaaac taaaaaatat
     9481 agatactaga gttttattag aaaaatgtta tgaaaaaaat gttatattta tgcctggaga
     9541 tttatttttt acagataact ctggttttga tactttaaga ctcggatttt ctaggttgtc
     9601 ttttgaagac attgaaattg gaataaaaat aattggagaa actataaatg aaatgttcta
     9661 ggttaactaa atattgttag cctagtttat ttttatgagg tataatatat atagtttata
     9721 tgaatctaat actgggggat aatatgagta aaaagtcaaa acgaagaaaa attaataaat
     9781 taaaattaac gattgtaata actgtattat ttcttgcttt attatctata tatgagatac
     9841 tgttctctaa tcccaacaaa aaaggtaata taaagagtca agaaactatg cagaataaaa
     9901 ataatacaac agatgattct aaaaataatc aaagctctac caaaaagcag gaagataata
     9961 atatcaagcc tactttttca tattcttcaa taccagataa tataaaaaat aaaatgattg
    10021 gtaaatctat gcctactgat gaacctataa gctttgatag tctatcttat ttaaaactta
    10081 catattatgg atttgatgaa aaaactcatc aaggagaaat gatagtaaac agtgaactag
    10141 ctccagaagt agtggatatt tttaaggaat tatacgaaaa aaaatatcca attgaaaaaa
    10201 ttaaattaat agatgactat gatgctgtag atgaaaaatc tatgtctgat aataatacct
    10261 catcattttg ttatagaact atagctggta caaatgtagt ttcaaatcat ggtaaaggac
    10321 gtgctataga tataaaccca ttacagaatc ctcaagtttc tggaaatgat gttacaccta
    10381 aaataagtac agtttatgct gataggtcta gcacaaaatt tggaatgata aaaaaaggtg
    10441 atgattgtta taatgctttt gtaagcagag gttggagttg gggtggctat tggaaaaacc
    10501 cagattatca acattttgaa aaataataga ttagaaataa aggtgaatta tatgaattta
    10561 atgacattgg aaaatataag taaaagctat tctgaaaaaa aattactaga aaatatatct
    10621 cttggaataa atgaggggga aaaaatagga cttattggtg taaatggtac tggtaaatct
    10681 accttactca aaattattgc aggagttgaa gaaagtgaga ctggtaatat aacaaaaact
    10741 aatggtatta ggatagaata tctacctcaa aatccaactt acaatgaaaa ttcaactgta
    10801 cttgaacaag tatttaatgg aacttctgaa gaattaaagc tacttggtga atatcaagaa
    10861 atcctagaaa aacttaattc aaactttaca gaggaattaa ataaaaaatt attatcagta
    10921 cacgaaaaaa tagatgcctt aaatctttgg gatttagaaa gtgaagctaa gtcagtactt
    10981 acaaagcttg gtataaatga ctttaatcaa aaaatagcag aactttctgg tgggcaaaga
    11041 aagagagtct ctctggcatc agcccttatt acaccttgtg agtttttgat actagatgag
    11101 cctacaaacc atctagacaa tgatactata gattggcttg aggaatatct taattcaaga
    11161 aaaggctcat tgcttatgat aactcatgat agatactttt tagaccgtgt tacaaataga
    11221 attattgaac tggacaaggg tagattattc agttatgatg ggaattattc aatatttttg
    11281 gaaaagaaaa tggaaagact ttcgctagaa gcaagtattg aagataaaag acaaaattta
    11341 ataagaacag agcttgcgtg ggtaaaacga ggagctcagg ctcgtagcac taaacaaaaa
    11401 gctcgtttac aaagatttga tgaacttatg aatagagatt cttttaaacc tgatgaaaat
    11461 ctcgatatat ctgttgcatc aagtagactt ggaaataaaa taatagaaat acacaatata
    11521 tctaaatcat ttgaagaaaa tactgttata gataatttag aatacacttt aactcgtaca
    11581 gatagaattg gtataatagg caaaaatggt atgggtaaat ctacccttat aaatatactt
    11641 aatggagaaa tagagcctga tagtggatat atttcaattg gagaaacagt taaaataggt
    11701 tgtttttctc aagacgactc tcatatgaat attgatatga gggctataga ctatgtaaaa
    11761 gaagctagtg actatataga aacttcagat ggtactagga ttacagcttc tcaaatgtgt
    11821 gaaagattcc tttttaatgg tactatgcaa tatactttga taggtaaatt atctggtggt
    11881 gaaagaagaa gactacatct tcttaaaatc cttatgacag ctcctaatgt gcttctttta
    11941 gatgaaccta caaatgattt agatattgaa acgcttaaaa tattagaaga atacctagac
    12001 gaatttaaag gtgtagtagt tacagtatca catgatagat atttcttaga tagaatttgt
    12061 aataaaatat ttgcatatga aggtaatgga aaaattcata tctatacagg taattacagt
    12121 gattatctta tttatagaga gattcaagga attgaatttg aagaatctaa aagggaatca
    12181 gttaaaaaag atgactctac taccctaaaa aaagaaaaac ctaaaaatga taaaaagctt
    12241 aagttctcat ataatgaaca aagggagttt gaaaatattg actctgatat agaaaaactc
    12301 gaatctaaaa ttgcaacact tgatgaaagt actgcaaaat tttcaactga ttttactaaa
    12361 cttcaagaaa ttttagatga gaaatctaaa ctagaaaagg aacttgaata caaatatgaa
    12421 agatgggaat atttaaataa tctagctgat gaaatagcta ataataagtg atattaaaat
    12481 aatcttaagt ttaataagtt ttgctttaaa tctatacttt ataaatattt taaaggtagt
    12541 gtctcaaaat gaacttttta gttttatgag acactctttt ttatgagaaa tcaaatatgt
    12601 tttcatcatt tcaacaatac aaaagaggct tatctctatt ttgagacagc ctctttcata
    12661 attaccttct aatttatatg ctttaatata tctataataa ttcttattat atctatatat
    12721 ccagtctttc aatatagtga caattaactt caatatgatt taaaaatact aacaaccttt
    12781 cataaagtgg atgagattct tctccatatt tagcttctag gttcttgcct atttctctaa
    12841 cagtcctttg accatcaatt tgtaaaaaaa caaaactgga atattcatct agttctattt
    12901 ttttgtattt aggtattcta gcttttaact ttctaaaaaa cttttgaatt ttatggtcct
    12961 gtttttcaag tatagtaaca acactatttt cagctacttc atattcaagt ccatcagtaa
    13021 ttttaaatac aatatctaaa acatcattac tcttgctcat tattttttac tttagataaa
    13081 acaataggta atgttgtaag cacaactaaa actactaata aagcaattgc cattgaattt
    13141 gttgcagcaa atccacttgg tacctttgga gttacaactc cagttacttg taatattata
    13201 cctattaaac caatgataga accaccagca acaagaccag aagataaact tataccatta
    13261 gaaactttaa cttctttttc tttttcagtt ttagatacta attcaacaaa taaacgaatt
    13321 aatgctccaa ctaatattat agaagttgtt gcaattggta aatagaatcc tatagctata
    13381 gtcattattg gtaaatttaa tgcatataga actattgcca taaatactcc aacaataatc
    13441 ataacccaag gtaaactacc tgacataata ccagaagtta atgttgacat taagtttgct
    13501 tgtggtaatg caaattgtac attatcacca gttgctctaa gttggtcaga aagtaataaa
    13561 attactccta caacaactat aacaccaact atacttgcaa ttgtaaagta acgttgcatt
    13621 tcgtttttgc taccaccaat aatataagtt actttttgag attgagtata acctccagct
    13681 atagcaatag caacaactat aaatgaacca aataaaagta atgatttatt agcttctaaa
    13741 tcagtccatc ccattataac aaacactaat gttacaataa ctagagaagc tatagtcata
    13801 ccagaaactg gaagatttga tgttccaatt gttcctgtta aacgaccagc tactatgaca
    13861 aataataaag ataataaaag agatatgata gctccaataa ttgccataac aatgttacca
    13921 gaaattaaga atgctgctaa aaatccaata actactccac ctaataaaag tatcatttgt
    13981 atagatgaac cttcttctcc ttctccagca cttgatttag ctttaagtgt ttcttttata
    14041 gaagcgatta tagttggtat aagttttatt gcaccaataa tacctccaca aagcatcata
    14101 ccagcaccta tatattttac ataactacta gatattgctc cgacatccat ttggttaaga
    14161 ggcattgcag gattattcca taccatagct ccatctttag ccatatctgt aaagtatcct
    14221 attaaaggtg ctataccaaa gtttgataaa atacttcctg caaacattgt taaagaaact
    14281 tcaagcccta ctataaatcc aattcctaaa agtagaggat ttacttcaat atcaaatttc
    14341 catttgtaga atttgcttcc tacaagactc attacattgt ttgctacatt taagaatgaa
    14401 ccagttaata cagtaataaa tccacttatt acaaatccaa ttcccatgta cttaattgaa
    14461 tctccaccag cctctgaagc aacaagtgtc tcagatatag ccattgattc tggatacata
    14521 agtttaccat gttcttcaac tattagatag ttgtgaacta tagcagcaat tcctatacca
    14581 aataaaactc caccaactcc aacagctagg ccttcaaaga atgtaatttg gcttccaatt
    14641 aatataactg ctggtaatac gaaaataaat ccacttgcaa cagattctcc accacttgac
    14701 atcccttgga ttaagttttt accaagaata ccctttgaac gagcaaatgc tccaacaaaa
    14761 gcagaaccta ttatagctcc tggaatacct gcagcaactg taagaccggc tttcattcca
    14821 gagtaagttg tagatgcggc aaaaatggca gctaaaataa taccgataat cagtacagct
    14881 acatttccac cggttctgga cttatctgtg atatatggaa cgtaatcttt tccactaacc
    14941 tcaccataag cacccttagg taattttttg ttcataaaat tttcacccct tttcaatttg
    15001 tatatattat aacatatata catataaaac ttaacttttt attattttac aaatagcgag
    15061 tca
//
LOCUS       DACTGW010000080        14961 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.080, whole genome shotgun sequence.
ACCESSION   DACTGW010000080 DACTGW010000000
VERSION     DACTGW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.080"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(3..2123)
                     /locus_tag="I9573_001920"
     CDS             complement(3..2123)
                     /locus_tag="I9573_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4796543.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVVDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHIARKILSINANEMIDSFVGDCIKDINFQNILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERAADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLGK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            2356..2757
                     /locus_tag="I9573_001921"
     CDS             2356..2757
                     /locus_tag="I9573_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796544.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            2800..3093
                     /locus_tag="I9573_001922"
     CDS             2800..3093
                     /locus_tag="I9573_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAT4796545.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            3108..3548
                     /locus_tag="I9573_001923"
     CDS             3108..3548
                     /locus_tag="I9573_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HAT4796546.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(3640..4113)
                     /locus_tag="I9573_001924"
     CDS             complement(3640..4113)
                     /locus_tag="I9573_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAT4796547.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(4113..4901)
                     /locus_tag="I9573_001925"
     CDS             complement(4113..4901)
                     /locus_tag="I9573_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAT4796548.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            5229..6473
                     /locus_tag="I9573_001926"
     CDS             5229..6473
                     /locus_tag="I9573_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAT4796549.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGIHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLINKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            6796..7893
                     /locus_tag="I9573_001927"
     CDS             6796..7893
                     /locus_tag="I9573_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAT4796550.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEITIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            7884..8582
                     /locus_tag="I9573_001928"
     CDS             7884..8582
                     /locus_tag="I9573_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAT4796551.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            8585..9649
                     /locus_tag="I9573_001929"
     CDS             8585..9649
                     /locus_tag="I9573_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAT4796552.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            9832..10536
                     /locus_tag="I9573_001930"
     CDS             9832..10536
                     /locus_tag="I9573_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4796553.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            10529..11851
                     /locus_tag="I9573_001931"
     CDS             10529..11851
                     /locus_tag="I9573_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4796554.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            12038..13411
                     /locus_tag="I9573_001932"
     CDS             12038..13411
                     /locus_tag="I9573_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796555.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIVMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(13784..14722)
                     /gene="pdaA"
                     /locus_tag="I9573_001933"
     CDS             complement(13784..14722)
                     /gene="pdaA"
                     /locus_tag="I9573_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HAT4796556.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDGGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         5501 a         1830 c         2379 g         5251 t
ORIGIN      
        1 gtttaactgt ttaacttgtt agacctattt atatttttta gaaattctac tccttttgat
       61 gttataattg aacctccacg accaacaagt atgtctacaa gcccaaagtc ttgtagttct
      121 aaaagcatat ttctaatctg ttgttctgtc aaaaatctat tttcctctaa agctatttta
      181 cataagcttt ttctacctat tctttctttt aaatcataag ccttttttaa attatccaat
      241 ataaatatgt attcatctag tgacctttta aaattatagt cgtatttcat gatgtttttg
      301 tctttgccaa tgttgaattg atactttttt atattatcac ttactttatt acaaacggtt
      361 ctactataat ttgaatctat agtgtccaat atatattcag gtaaatcaca tatttctata
      421 atatcttttc ccaagtaaca gaagtattct cctaagtttc ttaattctct tacatttcct
      481 tcccatctat acattttaaa gatttctttt acttcctcac ttagaataaa attacatgga
      541 atatcatatt ttaatgaacc aaaaatttca aatatatcag cagctctttc cctaagtgga
      601 tgtattttaa ggggaagaac attcaaccta aaatataaat ctcttctaaa catattttta
      661 gatacaagtt cttttaaatt tctattagta gctgcaatta ttcttacatc aatatcaatg
      721 acattattag aaccaatacg tcgtacttgt ttttcttgta tgactctaag taatttagat
      781 tgagaattta aatccatttc tccaatttcg tctaaaaata tagttccatt gttggctaat
      841 tcaaataaac caattttccc acctttttta gcaccagtaa aagccccttc atcatagcca
      901 aataattcac tttgcagtag attttcttga aaagtactac aatttactgc gacaaaggga
      961 ccatcttttc tcctagaagc actatgaatg gattgagcaa ataattcttt accagtacca
     1021 ctttcaccaa ttataaggat agatgaattt gattgagcca ttttattagc aatttttttt
     1081 gtattaatag tttgtatact agaacctaat atatcatcaa atgtgtattt actgatatta
     1141 ccagagttta ctagttgtgc tcttaattta gcttgctttt tttctaattg agaaaacttt
     1201 gtcatcttta gtataaatcc atcaaataca tttaattgta tatgtttaat ctctaaattt
     1261 atatcaatat gatttatttt aatcaatttc tcaaaaaatg gagttttatt ggtcaatatg
     1321 ttttgaaaat tgatgtcttt tatacaatca ccaacaaatg agtcaatcat ttcatttgca
     1381 tttatagaca aaatttttct agctatatga ttatagaatt gaattattcc atcattattt
     1441 gtgcatataa ttccatcatc aattatagat agtaacaaat caaattgtct ttcaaactta
     1501 tttgcatcta acaataactc acctgtacta taacttgtag gaattatttt ttctacgtat
     1561 ttttttatta ggtcatcttt tattagatgt tttaatttgg tttttgtagc aatttcaaca
     1621 atacaactta aatctattat tctgtatttt aaatctacaa cttcacaatt tttaaactta
     1681 ggcaggattt tttcgccagg agttaggatt acagcatctg tgggagtaaa ctcaatctct
     1741 ggatagcagg gcaggatatt tatattgtct attcctaatc tatgtattaa agaaattgtt
     1801 tctattgcca tatctttact taagttatat acataaataa ttttatcttt ttctaatttg
     1861 gatatcatat caatactgtt cttttcaaat gtcaacttgg gaacaatgat ttgagcatca
     1921 tagtgagata attttactat ttcatcgtat tttaaataag cagataggag tataagtttt
     1981 tctttaaaaa actttaaatt tccctcttca aatgagtata aatttatctt tagattatca
     2041 cctaagaatt gccttatttg attttcataa atttctccag cgtctttttt taaacttaca
     2101 atagctaatt ccatttgttt cattttaacc tctcatacaa atttgactta ttttagatta
     2161 cataataatt aaattagatt aaataatatt atttaattat tattataaat ttattataga
     2221 ctacacattg aagtaaaaga agtagctaaa attattcctt ttatttttgt tttttgtatc
     2281 tatttggtat aatagtatta gagcgtataa taatttaggc aaaatttttt aaggaagact
     2341 agaggagaat ataaaatgaa aaaatgtctt tactgtaata gggatataaa gtcaccaaat
     2401 aataaattag ctataaaata tgatgacaat atgaatatat atccatgtcg tgcagaatgt
     2461 aaggaaaaaa ttgaagaata tatagcaaaa aaacctacat ataagtttat aaatatatta
     2521 gaggttcttt taggtgtggg cggaatattc tgctttttga gtaaatggac tattgcagcc
     2581 caagttgtac ttggaattga tgcactggtt atatttttat tcccattggc aggatttaag
     2641 atgaatggaa agttaagtat ggaacaaact aagaatcaaa gtaggtctat tgcaatatca
     2701 attttggcat ttatagtaat aataaccgta ttatatttta aacaagttgg taagtagagc
     2761 ataattgtat aagacatcat tagagtatga ggtgagttta tggaagaaag aaaattagca
     2821 ccaaacaatg agagggaagc tctcataaaa agattaaaaa gaatagaagg tcaagttaag
     2881 gggatacaaa agatggtgga acaagaacgt tattgtgttg atatattggt tcaaatatct
     2941 gctataaggt ctgctataaa tagagtaggc acaattatct tagagaatca tataaaagga
     3001 tgtgtagcag aaagcattaa acatgatgat gctgataaaa ctgaagagat gataaaagag
     3061 cttatggata ctataaataa atttacaaaa taaggaggcg gttagttatg aatcaacaag
     3121 gatttattat ggaaattgtt gatgatagaa ctgcaaagtt aaaaatgcaa agacattcag
     3181 cttgtgctgc ttgtggaaaa tgtgctacta caaattcagc agagtcaaaa gaaatattag
     3241 tagaagtaga caacacaatt ggtgcaaaag ttggtgacca tgtagaagtg agtatggata
     3301 atatgaatgt attaaaagca gctgtaatgg cttatataat acctcttata gcacttcttg
     3361 taggaacaat agggacttat tatatattgg gggcaattgg aatgacaact ggagttgaag
     3421 ccataagtgg attagttgga atttgtctta caataatctg ttatttatac ttaaagaaga
     3481 atgacaaaaa atttagagac agtagagaat ttataccagt aataacaaga atattaattg
     3541 acctttaaga taattataat taaaaaatag caatgtgctt tttaaataaa tactcaatac
     3601 gtatttgtag agtttaagta cattgctatt tttatatttt tattttgatt ttctgtaagg
     3661 aataaagttt tttctgattt tcaaaagatt caatgttctt acaaaagaag ttacaactaa
     3721 tacaccaagt gctattgcag cacttagaaa tagcgcatct gtaccttttg acatagccaa
     3781 agaataattt ttctgaatca aagacaacat tgtattatat agcccaattc ctggtattag
     3841 aggaagaata cctggaatta cgaacacaat agctggttgc tttagttttc ttgctaaagt
     3901 ctcactacac gctgagacca aagcagcagc aataaaacct gcaaaaactg catttgtagt
     3961 aaaattaaat aaataaacat atacaatcca accaattccg ccagttactc cagctggaag
     4021 aagtagatat agtggagcat taaagaaaac agcaaatcca gcagttgcaa cacctgaaaa
     4081 taaaaaatgc aaaatcatag acatatcagt cattagaaag gacctcctat tctataccat
     4141 gtatcaagga caaaaccaac tccagaagca attgcaataa taatcatagc agcatcaaaa
     4201 gcacgagaaa ttcctgatat taaattccct gatattaaat ctctaatagc ttttataaaa
     4261 gcaactcctg ggagtagtgg cattatagcg ccgactataa gcattttagg gtcatctaaa
     4321 atgcctattt caactagtag aactcctgaa agtgcaatta aaaaagaaga tactaaacag
     4381 gaaaatgcag atattgcact tatcttcatc gttttatcat aagtaattgc agcaaagatt
     4441 gctattataa acgtaagtat aaaatttaat acataattcc ctccaagcaa acaagcaaaa
     4501 caagcagatg caattcctgt acaagagtaa aataaccata aaggataagg ttttacatct
     4561 tggatttcat aaagacgtgc aatggcatta tcaatatcat actctttgtc actaacaaat
     4621 tctcgagaaa aactgtttaa taaagaaatc ttgtttaagt ttatacctct tgattgaatc
     4681 gttttcataa acgaaaagcc atcaaatttc tcatctgaaa taattattac tgtaggagtt
     4741 gtaaaaatat ttacatgata aaaccctctt gacctgcata ttctaaggac actgtcttca
     4801 accctagatg tttcagctcc attttttatc atcagttccc ctacaaatag agctagtttt
     4861 agaatatttt tcttatattc aagttcctca ttttgattca taaatacacc tccaaattaa
     4921 aaaacgtaat attgaaatta tatcaaattt ttaagtaata ttccttaata tctatataaa
     4981 tatattatct tttttttgtt tcaaaatagt cacatttaaa ttatatccaa ttaaaagaat
     5041 tagacaatat tatttattat attttaactt aaaaataaag aaagtaacta tgttaaatat
     5101 ggaaaaatgg caaaacactt gactattaat tggtaaagga ctataataat aaattatcat
     5161 attgataaaa acgaaaatta tgaaatgtct gtaaaaataa aatttaaata ataactagga
     5221 ggagtaatat gtttgagaac ttgaaaaaag cggatcctga gatttatgaa agtatgaaaa
     5281 gagagttaaa aagacaacaa agaaatattg aattaatagc atctgaaaac atagtatcag
     5341 ttccagtaat ggaaactatg ggaagtcatc ttacaaataa gtatgcagaa ggttactcag
     5401 gcaaaagata ttatggtgga tgtgaataca tagatgaagt tgaaactctt gcaatagata
     5461 gaatcaaaaa aatatttgga gcagaacatg ctaatgttca acctcactct ggagcaaatg
     5521 caaatattgg tgtatatttt gctatgttaa aaccaggtga tgttgttatg ggaatgaatt
     5581 tatctcaagg tggacattta actcatggtg ctccagttaa tatttctggt caatattata
     5641 aattttatga atatggtata gataaagaca gtggacttat agattttgat gaagttagaa
     5701 agatagcaca tgaagtaaaa cctaaaatga tagttgcagg agcaagtgca tatcctagag
     5761 aaatagattt taaaaagttt agagagatag ctgatgaggt tggagcattg cttatggtag
     5821 atatggctca tatagcaggt cttgtggctg ctggtattca ccaaaatcca tgtgaagtgg
     5881 ctgattttgt tacaacaaca actcacaaaa ctttaagagg acctcgtggg ggtgttattc
     5941 tttgtaaaga aaaatatgct aaagacatag acaaagcaat attccctggt attcaaggtg
     6001 gacctcttga acatataatt gcttctaagg cagtttgctt taaagaagct ttaagtgatg
     6061 agtttaaaga atatcaggta caagttgcta aaaatgcaaa agccttagct gaagaactta
     6121 ttaaaagaga ttttaaattg atttctggag gtacagataa tcatttaata ctattagatt
     6181 tgattaataa aaatataact ggtaaagcag cagaaaagag attggatgat gcttatataa
     6241 cagctaataa aaatacaatt ccatttgatc caaatggtgc tcttgttaca agtggaataa
     6301 gacttggtac tccagctgta actactagag gtatgaaaga agaagatatg gcaataatcg
     6361 ctgaggctat agacctatgt ttaacttatg atgaagaatc taaggctaga acattggttg
     6421 ttggtttaac tgaaaaatat cctttatatg aagaatataa tattatggat taattataaa
     6481 gaacaactaa ataaaataca tttaaataat tacaatgatt gtgtatgtat ttgaatatat
     6541 ttagatataa aaaaggtgtc tcaaaatgaa ctttttagtt tatgaggcac ttttttgtat
     6601 ggaatcgaac atatttgtgt tatttaaata ataaaaaata tggttatctc tatttttaga
     6661 tggtctcttt gtatttttta attaaaactt taagatttct taagaattat actaggtttt
     6721 tctaaagatt taaagtctat acttataagt atagtaatca tatgaaaaga aaagctttaa
     6781 cgaggaggga atcttatgaa ggtattaatt cttacaggaa aatttggtat gggtcattac
     6841 tcagcatcaa attctttaag tgaagatata aaagcaaaat ttgataattc agaaataaca
     6901 attaaagata tatttgaata cattatgcct aactattcag ataaaatgta caagactttt
     6961 tctattcttg taaatcgggg aagtagtctt tataatttgt tttataaatg tgctgaaaat
     7021 ggaaagaaag atataaaatt tactttttca gattattttt taaataaatt agatacatta
     7081 cttcatgaag tacaaccaac agtagttatt tcaacttttc cattttgttc tcaattagta
     7141 tcaagatata aggaaaaata taactcaaat cttcctttaa taacatgcat aactgatata
     7201 agtagtcact cagaatggat tagtaaaaat acagactgtt atttagtagc tagtaaatct
     7261 actaaggaag aacttgtttt taaaggaata gatgaatcta agattaaagt taatggtatt
     7321 ccagtaaaaa aagaatttaa aagaatagaa catgtaaatc attcaactaa aaaaaatatt
     7381 cttataatgg gtggaggatt aggattactg cctaaatcag aacaatttta caaagaatta
     7441 aatagtttag aaggagtaaa aactactgta ataactggta ataataaaaa aatgtactat
     7501 aaactatatg gtagatatga aaatatagag gtagtaggtt acaccaatga agtatataaa
     7561 tatatgaaag attctgacct tattatttct aaaccaggtg gaataacact atttgaaaca
     7621 atttattcag aacttccaat attggcattt aatccatttt tacaacaaga gatagataat
     7681 gctagtttta tattaaataa tgaaattggg agaatacttg gtaaaaataa aaaatactat
     7741 gtagatgaga taaaagattt aatttatgat gatgctactc ttaaagaaat gagcagtaac
     7801 atgaaagaat taaaaaaaca atttgacaat aataccttag agaatatttt attttcactt
     7861 gatgaacaag gagcgtgtag agaatgtatg tagtaggttt aattatcgtt gttgcattga
     7921 tttttttagt ccattcaatt atacctacat actataataa actattaaat aaagaggtat
     7981 taaaaaatat ggctggagaa aatgaaattg cacttacatt tgatgatggt ccagataaga
     8041 gatatacaga aaaattacta gatgtgttaa aagaaaatga tatacaagct atgttttttg
     8101 tagtagcaaa gaatgctgaa aaagagcctg aaattataaa aagaatgttg agagaaaatc
     8161 atatagttgg tttacattca ttggaacaca gaaatgcatg gctttatagt tatagttatg
     8221 ttaaaaaaga ttttattgaa agtacaaata taatgaaaaa tttaggtgta gatgtaaact
     8281 actatcgtcc accatggggt catactaata tattttcaaa ttcatttgtt aaaaaatata
     8341 atctaaaaat gactttgtgg gatgtaatgg cagaagattg ggagaaagat agtacagtag
     8401 acattattat aaacaagtta atgagtagaa caaaagaaaa ttctataatt tgtttacatg
     8461 atgcaggtga aaattcaggt ggtgcagtag gtgcacctga gcgtactatt gaagctttaa
     8521 agatagctat tccaaaactt aaagccagtg gtttaaagtt cgtaacacca gaaaggatgt
     8581 agttatgata agtaaaactg aaaagaaaaa taaatgccta ggtagtgtag cttttttagt
     8641 tttactaatg ggcataacag gatattttgt ttttagaggg cagtcagttg aatcattgat
     8701 aaaatcatta aaaggtgcaa gccctatgtt tatactaatt ggatttgcaa tgatgtttat
     8761 atatgttgcc tgtgaaggta taaatatata cttaggtatg aaagctttaa accaaaaaac
     8821 tactctttta aaatgtatgg gatatgcttt tattggattt tattttagtt ctattacgcc
     8881 ttcagcatca ggtggtcaac cagcacaagt ttattatatg aaaaaagacg atataaatat
     8941 atcatattct tcacttatac tattagtcat tgttgttatt caccaagtag ttatactggc
     9001 atatagtgga ataatgttca ttatggaacg tgagtttata ttaaataatg taagtggtat
     9061 gaacatttta ctaatatatg gtgtaataac aaatgttgct cttgtcattg gagtaattgc
     9121 aattatattt tcaaaaaaac ttgtaaacaa ttttataata tcaataacaa atttattagg
     9181 taagttaaga ataataaaag atgttgaaag ttcaagaaaa gttataatat ctcaaattga
     9241 agagtatgtt aaaggtgctc aatatataaa gcagaatcca aaacttgtag tccaaatttt
     9301 agtcataact attgtacaaa taacagctat gtttttagtt cctttctttg tatacaaagc
     9361 ttttcatcta tcaacatata ctgtatttga gatattagcg atacaatcct tgcttaatat
     9421 tgctgtttct tcattaccat tgccaggggc tgttggagct tctgaaaata gttttatgac
     9481 attatttaag atatttttcc ctggtcactt attagttccg gcaatgttac ttagtagggg
     9541 aataagtttt tatgcatttg tagctataag tggattgata tgtatagtag tacatgtaaa
     9601 atcttctgaa aaagtaaaag cagtgaaaaa agttgcttat gtaagataat attatataga
     9661 ttatttttat taatatgtca atttgtgtgt acattttaat taagttttaa acatgtttac
     9721 aggagtataa actattaaaa cgtgtgacat tgctaagctt ttgctaataa agtataatat
     9781 tttatatagt ttgaaatatt taagaaatat agcaaaaaaa ggagcttggt gatgaataaa
     9841 gagattccaa aaaaaatttt agtagctgat gataatcctg agattaggga aatcgtagaa
     9901 atacttttaa ctggtgaagg ttatgaagtt attatggcaa cgaatggaga agaagctgta
     9961 aacttagtag atagtacgat tgatttgatt attttggatg ttgtaatgcc tgtaaaaaca
    10021 ggatttgttg catgtaatga aattagagaa aaaacgacag caccaatatt atttttaact
    10081 gcaaaaactc aagactctga taaagttatt ggattttcag caggaggaga tgactattta
    10141 tcaaagcctt tttcatattc tgaactaatt tcaagggtta agtcgctact tagacgttat
    10201 tatatatacc agggaaagga aaaggaaaga gagccaaata tattaaaaac aaaagagtta
    10261 tgtgtcaatt tagatactca agatgttaca ctaaatggag aacctgtgtt tttgacaact
    10321 atagaatata gtatattaat tcttatgttg aaaaatagaa aaaaagtttt ttcgtctgaa
    10381 aatttatatg aaactatttg ggatgagcag tatttttata cggcaaataa tacaataatg
    10441 gttcatatta gaaatttaag aaaaaagtta gaaaaagacc aaaaaaatcc acaatatata
    10501 aaaaccgcat ggggaaaggg gtattatatt gattaacaaa attaagccat ctaagttggg
    10561 agttatacta ttgatatatg tagtaatctc agctgcagtg gctttcattt taaatatacc
    10621 attttactct gctgttgaat ctatagtaaa agatgtatca gaagggaaag tatattttaa
    10681 aagagaagta aatcaactta tggataattt tcaaacttat gtgaataaca atggtataag
    10741 agcagatgat ggagatgata tagcaaaatg gaatagtgat aattggtttg tttctatgaa
    10801 gatatataaa ggaaataagg ttttttatga tacattatac tatcctaatg aagtacctga
    10861 taagagtgag gaaaatgagt tttatagcag tcccatggct agaaactatg agatacaatt
    10921 ttctgatatt aaagctaata tagttgtagt agctttattt agatttagat ttcaaaattt
    10981 tatagaagca atttctatta catttatggc acttacattt attataacat ttttactgtt
    11041 gtttagtaga aagataaagt acctaaggaa aatagaaatg ggtataaaga tagtagagag
    11101 tggcagttta aagtatagaa tcccagtaaa aggtaatgat gaattatcat cacttgcatc
    11161 tagtattaat gagatgagtc atacattgga gaatcaactt ataaaagaaa gggagatgaa
    11221 gaataaaaat aaagatatag taacttcgat atcacatgat gttagaactc cattaacatc
    11281 agtaatttgt tatttagatt tgatcaaaga ccataaatac aatagtcctg agcaattgga
    11341 acaatacata aaaaatgtaa ggtctaaagc atatcaaata aaagatttaa cagatgacct
    11401 ttttacgcat tttgttaatt ctggagagga agtttcaaaa gggtatgaaa aaataaatgc
    11461 taatgaattt atttttcaat tacttttaga tagttcatat acattggagg agaaaggatt
    11521 tcatataaca atagaaaatg aactttataa ccaatatcat atttatgtag atgttacaca
    11581 atttagaagg atatttgata atttatgttc taatataata aaatatgcta ggaaagataa
    11641 gcctatagta tttttaatta aagtagagga ttctaaatta ctaattatac aaaaaaatag
    11701 agtaagtaat ttttctaaga atgtagaaag ttttggaatt ggtataaaga attgtgagaa
    11761 aatagttgca tatcataatg gaagcataaa tgtaaattca gatgataaag aatttgctat
    11821 tgaaatatct attccagcaa aagaatcata aaaatttaaa ttatatattg tgtaattgct
    11881 attatatatt gtgtaattgc ttatgtaaaa ttctatgaag caggaagcat gtgactttag
    11941 ttatgggatg ttcaatattg tgtacctcta ggcaagtaac aagtttactt acaagaccat
    12001 ataatattat tatcttaaaa aaaggggaca taataatatg gttttgacag gagcacatta
    12061 tatttacatt ctatttatgg taattatatt aattactatg attatgaaaa aggatacaat
    12121 agtaccatgt atattaggtg ttttttttat gggattgttc tttgaaaaaa acatctttgg
    12181 tgctataaca gcagtgttta actcttttat tatatcttta aatgagttgg gtccaattat
    12241 cttgattata gctattatgg ttgcattatc taaagcccta gaagccaata atgctataca
    12301 gtatatggtg agaccatttt cgagagttat aaaaaattct aatacagcat tttttgtaac
    12361 tggatttgtt atgttagttc tttcttggtt tttctggcca actccagctg ttgccttagt
    12421 tggagctgta tttttacctg ttgctatgag agctgggcta ccagcaattg gtgttgcagt
    12481 agcattaaat ttatttggtc atggtcttgc cttgtctaca gactttgtta ttcaaggagc
    12541 tccaagtata acagctggtg cagcaggtgt tgcagtatca tatgttataa atgatggtat
    12601 gattttgttt tgggtgatgg gaatcgtaac catatctatg gcattttata cattaaaaag
    12661 agatattaac aaaggaatgt ttagagaaga actaaaaggt tttgaaagtg aagaagtaga
    12721 agagttcaat ggaaaatcta aaatagcaac tattttagta gctctgggat ttcttgctga
    12781 tataattgtt atgtatatat ttgacctaaa aggaggagat gcatcagctc ttttgggtgg
    12841 aacagcagta tttttaataa taattataaa tgttataaat tttggcaaag atagtcttga
    12901 aaatgtatgt gagaatataa ttgatggttt tgtgtttggg ataaaaatat ttggagcaat
    12961 tataccaata gcagcatttt tctatatggg tgaagttgcg cccttgacag gtgtatttgg
    13021 aaaagtatta gcaccaggtt cacaaggttt attatcagat ataggcatag ctctttctca
    13081 aacaatacca cttaataaat ttgccgtatc aggtatagaa actgtagtgg gggcaataac
    13141 tgggcttgat ggttctggat tttctgggat ttcattagtt ggttcacttg cctctgtatt
    13201 tggaactgct ataaatgcaa gtgttggtgc attagctgca ttaggacaaa tcagtggtat
    13261 atgggtaggt ggaggctgtc tagttccttg gggtcttatt tcagcagcgg caatttgtgg
    13321 ggttagtcca atagagttgg caaagcgaaa ctttgtccca gttataaccg gattggttgt
    13381 aacaaccata gtagcaatgt ttattattta ataaatcgaa taaatcttta attgtacaaa
    13441 ttaaaaatag atgtgtagct ataatttatt ataattgttt tactaatttt aagccataca
    13501 tctattttta ttgatatttt tattagtatt ctttaataaa ataatatttt tcattatact
    13561 atacataaca tctaaatata acatctaaaa tactgttatc tatgtttaat atcttgccaa
    13621 attttttgat gttttattac cctgtcccac caacatatat aagctatcgc aagtttttta
    13681 cctatactat caccaaacaa aaattttaat tttaatttat tattttataa tctatattat
    13741 attaactata ttagtttgta gactttagat gtaattctaa atattaatac tccaagagtt
    13801 caaattcata tcctagtttt cttgcatttg atataaaatc atttaaaatt tctgtactag
    13861 ttttggaaac tgcatgtagt aacaagatag aaccatcatg tagattatcc attatttttt
    13921 gcgttgcttc ttcatgtgat ggttgtttat cagtatccca gtctctgtat gcaaaactcc
    13981 aaaatacagt cttgtatcct aaatttttag tcattgccaa gcttttttct gagtattttc
    14041 ccataggagg tctaaaaaat ttaaccatat ctttcccagt tacatcttta tataactttt
    14101 caacatcata taattcgtca ttaaagtttt ttagattgct tgtttttgtt ggcatagatg
    14161 gatgggtttt actatgattt ccaactatat gtccctcaga aaccattctt tttactaaat
    14221 ctttattttc ttttatatat ggagctgtaa caaaaaaaac tgctttaacc tggttttgtt
    14281 ttagagtgtc gagtatcttt gtagtgtatc cattttcata accttcatca aatgtaagat
    14341 atagtgtttt ttgaccatct tttgtaggtc cattgtatag ggcatcatag tcagaaaatt
    14401 taaattcaat gtccgtattt acttctggtg ttttatgctt attgttaggt atataaaacc
    14461 aatcaagagt agtgttatct aaagaagata catctaattt attattatca ttattagtag
    14521 tggcttttga tggtttatct gttccgtctg aaactattac attggaagta tccttaccac
    14581 catctatttt ttccttatct tcttgtaact gtgtttcctt attttgattt tcgttttgat
    14641 tgttttgtga atttgaacat ccaacaataa aaaaaataga aatagaaagt agtgttaaat
    14701 aatgcaatac ttttctaaac attttgaagc tcctttctaa attaataata attgcatata
    14761 gtattattat aattggaaat tactattaat aatagttata tctttaaaaa taaagtcaca
    14821 atagtatgac aaagggtgaa taatcaataa atttaaaaaa aaatgaaaaa aatagttgac
    14881 catattatga gaagatgtta ttataataaa tgtgctcaac aagaagtaac aaaataacaa
    14941 gcacaaagaa gttaattgaa t
//
LOCUS       DACTGW010000081        14821 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.081, whole genome shotgun sequence.
ACCESSION   DACTGW010000081 DACTGW010000000
VERSION     DACTGW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.081"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(119..541)
                     /locus_tag="I9573_001934"
     CDS             complement(119..541)
                     /locus_tag="I9573_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAT4796557.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            1019..3922
                     /locus_tag="I9573_001935"
     CDS             1019..3922
                     /locus_tag="I9573_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAT4796558.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILVL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     ISIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3955..4401
                     /locus_tag="I9573_001936"
     CDS             3955..4401
                     /locus_tag="I9573_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT4796559.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            4524..4826
                     /locus_tag="I9573_001937"
     CDS             4524..4826
                     /locus_tag="I9573_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4796560.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            5007..6413
                     /locus_tag="I9573_001938"
     CDS             5007..6413
                     /locus_tag="I9573_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAT4796561.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFSDPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVISMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            6757..7815
                     /locus_tag="I9573_001939"
     CDS             6757..7815
                     /locus_tag="I9573_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAT4796562.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYASPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7890..8576
                     /gene="araD"
                     /locus_tag="I9573_001940"
     CDS             7890..8576
                     /gene="araD"
                     /locus_tag="I9573_001940"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAT4796563.1"
                     /translation="MLEILKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNVVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            8594..9241
                     /locus_tag="I9573_001941"
     CDS             8594..9241
                     /locus_tag="I9573_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HAT4796564.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELNVEHICVLDLNGNVIEVEKGIKPSSEVLMHIEIYKIRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFESWKYPEEIKF"
     gene            complement(9414..10781)
                     /locus_tag="I9573_001942"
     CDS             complement(9414..10781)
                     /locus_tag="I9573_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HAT4796565.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLRSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10971..11059)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11487..13607
                     /locus_tag="I9573_001943"
     CDS             11487..13607
                     /locus_tag="I9573_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAT4796566.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESILKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKEIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            13711..>14821
                     /locus_tag="I9573_001944"
     CDS             13711..>14821
                     /locus_tag="I9573_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HAT4796567.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFEDIETYSKDSQKKREKEL
                     DKFINTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEIYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTQV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGFMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYI"
BASE COUNT         5847 a         1689 c         2536 g         4749 t
ORIGIN      
        1 atagtgtcac atgtttatca atagtttttt cattaattat tgtaacgaat agtaggactc
       61 ataaaatact tagaaatatg tttttagata gattttaaat atataatcta acttatatct
      121 ataaattaac actattattc tccaattcca tttctagttt ttttataaat ttattaaatt
      181 cttctggttg cctttttcca aacaatactc ttacaagagc tgaattgatt tttccaaata
      241 tattcctctt gctctcactt tcaataaaaa taagttttgt attttctaag tcaatatgta
      301 ctaattcata tctagtaata gtactttctc ttttattgct tgtttttatc tcatatactc
      361 tatttttttc gaaattagtt atttcaacct taaatctttt attatttttt tgttgtacaa
      421 atgttcctac tgcttttttc tcattaaaat taggaaaatt tagtcttgca gattttgtaa
      481 atatattaaa tatgtctttt gcagaatatt ttaataaaac ttcagtttta aatgtattca
      541 tatcgttttc ccttctgtat ataaatttaa tatttataag ttaattttta agttaaaata
      601 gtataatatg ccatattaaa tcatgttaaa actatttata tagctaaaac cttacctata
      661 taagtatgta tactatcata taaaaataaa atcttaaaat agaaagggtt aaaaacacag
      721 tttattataa tttttattgt tgagtagata cgtactatta tatatgattg agcaagtttt
      781 tcaatcataa atttataaag tattttgcat taaaatttaa acaaagagtt aaattattta
      841 agattatgaa taactaatac atttagtaaa ttactaaata tacaaaatgt aaatacttat
      901 caaaatgtat aaatacttat gacatctagt tacttacagg gtttaagtag aaaaaaatat
      961 caaagtaagt atggcatgat agttgcttat atatttatca agaaaggagt tattaagcat
     1021 ggagttgaga gataaacttg taaacatcat aaatacagaa aataaaaaag atccattaac
     1081 agatgttcaa atagcaaagt ttttaagtac aactagagag aacataacta atttgagaaa
     1141 agaacttaat ataagtaatt caaggcaaag aagatatcct tatttaaaat cagcaatttc
     1201 ggcaatatta caaaagaata agaatatttc tatatcagaa attacaaggg aattgatgac
     1261 agaaggcttt aatatatcga gaagagttgt ggaggaactt ttacctaaag aaagtttaga
     1321 aatggatgta gtagaggaat ctgaagaaga atcgaatgac ccatttgaaa ctctgatagg
     1381 taataaaggg agtcttagaa atgctgtgga gcaggctaaa tctgctatat tgtatccacc
     1441 aaaaggatta ccaacgttga ttattgggga aagtggagtt ggtaagagtt tattttctag
     1501 acatatgtat gagtttgcaa agcaaaaaaa gatagtaaaa gaaagctcca attttgttgt
     1561 atttaactgt gctgattata gtgacaatcc acaattatta ttatctttgt tatttggata
     1621 taaaaaaggt gcatttacag gtgctgaata tgatacacca ggtcttgtag aagaagctga
     1681 tgaaggagtt ttatttttgg atgaaataca tagattgcct ccaaaaggtc aagaaatact
     1741 attttctatc ttagataggg gaaagtttag gagattaggt gaaactaatg ctgaaagaaa
     1801 agtaagtatt atgttgattg gggcaactac tgaaaatgta gagactaatt tattattaac
     1861 attcagaaga aggataccaa tgctaataac tttgccacca ttacatgata gattactaaa
     1921 agagaaaata gaccttatat acaatatctt tcaacaagag tgtaatagaa taaatgcaaa
     1981 aatatttgta gataaaaatg tgattgaaat acttgtacta aaaaagttta gtggaaatat
     2041 aggacaatta caaaatatga ttcaggttct ttgtgcaaga gcttttatga agtttataaa
     2101 ttccaaagat gaagatagtg aatcgatagt agtagtagat ataaatgaag ttttaaagct
     2161 taaagattct tttaaagatg ctgcctttca agagattgaa tatacagaga taagaaaata
     2221 tttgaaaaat gctatattta taccatttaa tttagaagaa tcatcagtaa aaggaagtct
     2281 tttaagtgat gaatatatat taccagagga catatacaaa acaattgaaa agaaatatta
     2341 tgacctaaaa tccctagaca ttgaagatat agatatagag aatattttat ggacttttat
     2401 tttaaataga tttgctgact taaagtttaa tctcgatagt aatgaagaaa ttttttcaat
     2461 gagtgattta aatagttttg taagtgatag tttagttaaa ctggtaaaag aacttatgaa
     2521 tgatatagtt gaaaaaaata tgaatttaga agtaaataaa aatatgttca aatatctagc
     2581 aatacactta gaagaagcta taaaaagaat taagctaaat caaaagataa taaatgtaaa
     2641 tttagaaaaa ataaagattg acttttcaaa agaatatgaa attagtaaaa gttttgcaat
     2701 aaaaattgag gaatcaatta gtattaagat tccagatgat gaaattggat ttataacact
     2761 ctacataaag tctgctctaa aaaatgaagt gaaaaaagat aaagtagggt taataataat
     2821 ttctcatgga cgtatagcta cagagactgt aaatgtagtt aaagaacttt taggtgtaaa
     2881 gtttcctgtg gctatagata tgcctttaga tgaaaaacca ataaatatat acaataaggc
     2941 agtagaactg tcaaaaataa tagaccaagg gaaaggtata ttgtttttgg tggatattgg
     3001 ttcacttaca aacataggtc aaatagtaaa taagagaacc aatataagca ctaaaactat
     3061 tgatagagta gacctcctaa tggcactaga agcaactaga aaagtttcta tgggagagga
     3121 agaacttgat gaaatatttt tttctttgct aaaagatagg atgggatata actatgaatt
     3181 agaaaaacac atagataaat caaatgcaat tgtaacatta tgtcttactg gagaaggaac
     3241 tgctaagtat ataagtaaaa cgttggaaga aaagtatgat aacactaaat gttatcaaat
     3301 gagtgcattg gatgaaaatt tattttgtaa aattgaaaat ctaaaagaga caaacaatat
     3361 attagctatt gttgggacaa taaaccctaa gataccaggt ataaatttta taccctataa
     3421 taaagaactt ttcaaaaatt tggatatcta tctatcaaaa tcaaaacaac aagatgacag
     3481 accagttaaa tcgtatgaaa gaatgcttga tgaggattta gtgatttttg aaccagatat
     3541 atattttaaa aaggatttat tagaatatgt atgttcaatt cttataaata aagaatatgt
     3601 agaaaaagat tacttagatt ctgttttaca tagagaagaa atgcttccaa catattctaa
     3661 aggagctata ggtgtgcctc atggagattc ttctactgta aatagtacaa ggtttgtttt
     3721 tgttaaacta aagaatccta ttgattgggg tgtaggtaat gtaaatttca tatttatgcc
     3781 agtttttcag gctaatgata aagagattgt taaaaatgtt cttgagatac taaaagatgc
     3841 agaatttatg aacaatgcca ataagtgttt tgataaagat gcttttagaa ggattatatt
     3901 tgataaattt aaacatctgt agccatagca aaaaaattga gaggagttag agtaatgaat
     3961 gtagatttaa tatcattggg tttagaatct aatgattatg aaagtgttat tgaagaatta
     4021 ggaagtataa tgtgtaaaaa agaatatgta aaagaaacat acataaatgc tgtcttggaa
     4081 agagaaagga cattaccaac aggtctagat attggagaaa tgtgtgttgc tattccacat
     4141 acagattcaa agcatgtaaa tgagtctaat gtagctgtag ggatattgaa aaatcctgta
     4201 aagtttaatt caatgataga cccaaaagat agacttgatg tagaacttgt atttttatta
     4261 gcagtaaaga acccagattc acaagtaaaa ctgttaaaag atttaatgtc agtttttcaa
     4321 aatataaagt tattaaaaaa tataaaaaac gcatcaacaa aagaagaagt agctaaatta
     4381 ttggatttta tagaaattta atgtaggaga attttataaa taaaaaatta aaatttcaac
     4441 aatcaaagta agtattattt ttttaatagg tttaatctta tataaaataa aaatttagag
     4501 tgaaaaatta ggaggaaatt attatgaaac aatttaatgt attatcagtt tgtggttcag
     4561 ggacagttac atcatctatg gtagcaggta aattaaaaga agtactaggt aataagggga
     4621 tttctattac tacaacagaa gctagaccaa cagaagcact aaatttagca caatcaggaa
     4681 gatttgactt tataacattt acgagtccgc ttcaaacagg cgattatgga ataccaacta
     4741 taaatgcatt tgcttgccta actggtatag gggaagaggc attctttgaa gaagttttga
     4801 ctgttattga aggcatagat aagtaagttt tggagacgtg aatttatagt atttaaatca
     4861 atgatttaga tactataata agctaatatt aactaaatat ttgtggaaaa aacattttat
     4921 atgttgtcat aaattaaaaa attataaaaa tagtcactta agtattaaat taagcttata
     4981 aatatttata agtaggaggg aaaaagatgt tagaatttat gaaatctata atcgatacat
     5041 ttggttctgc aattatagtt ccaattataa tattcataat agcaaagata tttaaagtaa
     5101 ctactaagaa atcgtttctt tctgctgtgt atgcaggagt tgctttacaa ggttttacac
     5161 tgattttaaa ttcatttaca ccaataataa caccagttat caataggatg gttgagagta
     5221 caggagtaaa cctaccagtt tttgatgttg gttggcaagc aacatcattg gttgcatttt
     5281 caacaagtgc aggtatgata tatttaggtc ttggtatatt attacaaaca attttatttt
     5341 tgataaaatg gacagacgtg tttcaaccat cagatttatg gaataactat tcttatatgg
     5401 tatggggagc aatggttata ggagtaacag gaaattttcc tttaggtata gcatgtatgg
     5461 ttttattaaa cttatatagt ttattaatat cagagttagt tgcaaagaga tggtctagtt
     5521 actatagata tcctaattgt acaataatag ctatgcataa tgttgaggct tctgtgtttg
     5581 cagtattttc agaccctata tacaataaat tgggattaaa taaaataaaa ttaaatccaa
     5641 aagaattaga gaagaaatta gggtttttag gtgaaccaat tactttaggt ctttttttag
     5701 gtatgtttat aggtatatta ggtaatatga cgagaataaa tactatggaa gcttggggag
     5761 aaataatgaa agtaggtata tctacaagtg cagtgatggc gattttccct aaagtagcaa
     5821 gtatgtttgc tcaagcattt gcacctataa cagaagcagc tagaaagatt atgcaaaaag
     5881 ctggaaaccg tgagtggtac atagcagtaa atgatgctgt tggatatggt gaacctgcaa
     5941 ctttaatatc aggtcttata ttaattccaa taatgcttgt aatatctatg gtacttcctg
     6001 gtaataaggt attaccagtt gtggatttgt tggcaattcc atatatggtt caaggtttag
     6061 ttgccataca taatggtaat ataccaaaag tattagtttc aggtataatt tggtttggat
     6121 taggattata tgtttgtaca tctacagcac cattatttac agatatggct acaaatatag
     6181 gtgtagctat acctgcagga gcaatgttaa taacaagctt taatatacta ggaaaacctt
     6241 tgatgggatt agttttcttt gcatttctaa gtgcaaatcc aatttatata ggtctttcag
     6301 ttgtaattta ctttgttcta tgggcattgt ttagaaagaa caaaacaagt attcttgatt
     6361 atttagaaaa acaagcactt aaaaatgtag aagaagagcc tgtagcagtt taagtttata
     6421 aaaatgaaat aagaataatt aaaggagttt ataattgatt ttatataagt atcagataat
     6481 acctgagaaa tatttaaatg aaatatagac ttctttttat ttgataatat ttgtaattat
     6541 aatgcattgt atttttatag ttgagttttc tgtataacct gctatttaaa acatactctt
     6601 ttatatttat acagtaatgt ggtttaattt tataaaatgt aatctctact ggtaaaagac
     6661 tttgtatata aggcatttgc ctgaataaac catttatctt ttgtatggca tagatattgc
     6721 tatatagaag tatatagcat gaaaggagat atttatatgg gagataaaat gagagcatca
     6781 gtattgtata atgtaggtga tgtcaggtat gaaatggtag atataccaga aataacagat
     6841 acgcaagtgc ttgtaaatgt aaaatatgtt gggatatgtg gttcagattt gcctaggtct
     6901 atggtaagtg gtttgagtgg aaatacaaaa tatccattga ttttaggaca tgaattttct
     6961 ggtgaagttg ttaaaattgg agaaaaagta aagcatatta atgtaggaga tagagtagct
     7021 gttgcaccat tagtaccttg tggaaaatgt gattattgta atgaaggtaa ttttggcttg
     7081 tgtgatgatt acaatataat tggaacaaga gtaaatggag cgtttgcaga atatgtaaga
     7141 gttccagaag aacatatttt aaaactacca gatacattgg attatgaaac tgctgccgga
     7201 attgaaccag caactatagc ttatcatggg atttcaaagt ctaatattag agtaggagat
     7261 agtgtagttg tgttaggatg tggtccaata ggtcagtttg ttatacaatg ggcaaaggtg
     7321 tttggtgcat ctaagattat agcagttgat atttttgatg aaaaattaga actttctaaa
     7381 cttctaggag caaactatat acttaactca aaagaagtaa atgtaatcaa agaaataaaa
     7441 aaaataacaa atggtggtgc tgatgttgta atagagactg ctggtagccg atttacacaa
     7501 gaacaatctc tatttgtagc aaaaaagaga ggaaatatag tgtttgtagg tatatcacac
     7561 acagaattac cacttagtgc agacgctact gagtgtatat tacgtggaga acttaccttg
     7621 aaaggttctt ggaattcata tgcaagccct tatcctggaa gagcatggac tgcaacattg
     7681 gattttatgg gaaaaggtga cattattttt aaacccatga tttcacataa aataggtctt
     7741 aatgaagtgg gtgacttttt aagtaaaatg agcaagagag aaattaattt caataaaatc
     7801 ctagtagaaa tataaaaatt taataaaaaa ttctaaaaac aattaaattt tataactatg
     7861 acattaaata aaatatagga ggaagaaaag tgctagaaat tttaaaagaa gaagtattaa
     7921 aagcaaactt agaattacct aagaaaaagc taattactta tacttgggga aatgtaagtg
     7981 ggatagatag agaaaaagga ttagtggtga taaaaccaag tggtgtagag tatagtaata
     8041 tgactgtaga agatatggtt gtagttgatt tagagggaaa tgtagttgat ggcaaactaa
     8101 gaccctcctc agatactccg actcatctag ttttatataa agagtttgaa gaattggggg
     8161 gaatagtaca tacacattca agttgggcaa caatttgggc tcaaatagga aaatctatac
     8221 catcttgtgg tactactcat gcagattatt tttatggaag cataccatgt accagaaaaa
     8281 tgactagaga agaaatttgc aatgaatacg aaaaggaaac tggaaatgta atcattgaag
     8341 aatttatggg aagaaatcca attcattgtc caggaataat tgtaaatgac cacggacctt
     8401 ttacatgggg aaaagatgca aatgaggctg tacacaatgc agtagtttta gaagaagtcg
     8461 caaagatggc ttattataca gagttaatga gtcctgataa tattatggat aaggttctta
     8521 tgaataagca tttttcaaga aaacatggaa aaaatgcata ctatggtcaa aaatgattat
     8581 ctggaggtga attatgctag actctttaaa aggtaaagtt gtggaaatag caattaaagc
     8641 agagcattat ggtttatgta aagaaaagac tggtaatttt agtattagag ataaggatac
     8701 tggatatata cttataaccc catcaggtat aggaagagaa gaattaaacg ttgaacatat
     8761 ttgtgtattg gatttgaatg gaaatgtaat agaagttgag aaaggtatta agccaagtag
     8821 tgaggtattg atgcatattg aaatatataa aataagggaa aatataaatg ctatatcaca
     8881 tactcattct ttatatgcaa cagcattttc agtagcaaag aaaccaatta taccaattgt
     8941 atatgaatca gttaattatg gcggttatgt agatattgct ccatacgaaa gaccaggaac
     9001 tttaaaatta gctaagagcg tttcaaaatt actgatagat acagatgcat gcttattaga
     9061 aagacatggg ttgacaactg taggtaatga tttagacgaa gcattattaa aatcaagata
     9121 tgttgaagag gtagcagaaa tatactatag gtctttagta ttaaatcaat ttaaagaact
     9181 tgaagtagta gatgttgatg agtttgaatc ttggaaatac ccagaagaaa taaagtttta
     9241 atctaaataa ttttcattga agtggttaat taaaatatat gtattattta tatcaattac
     9301 tatgttttat tatattaatc accgtatttt gtaaatatat ttattacatt ttttcaaaaa
     9361 aatatagaat ccgaaagcca tataaaattg acttgggatt ctatactttt tatttatgca
     9421 tttttcttta tctgattttc tggagaattt atatgttcaa aatatttttt agtttcagat
     9481 actattatag gtgataatgc aagtaaagct ataaggttag gtattgccat aagcccattt
     9541 acaatatcag caattatcca aacaacttct aactttaaga aagcacctaa taacaccata
     9601 gccacaaata cagttctata cccattcatg ccttttacac caaacagaaa ttcaaaacat
     9661 ctttctccat aataactcca tcccaaaatt gtagtaaatg caaataatgt taaactaact
     9721 gtaagtaata ttggtccaaa tgtaggaatt gctccgttaa aagctgcttg tgtcataaca
     9781 gctccattta ggtctgaaca ccacactcct gaaataacaa gagagaatcc agtaagagta
     9841 cagattataa tagtatctat aaaagtacct gtcatagata taagaccttg ttccgctggc
     9901 cattcagttt tagcagcagc tgcagctatt ggagcactac ctagaccaga ctcatttgaa
     9961 aatacaccac gagcaatacc atttctaatt gccagtgaca tagttgctcc taaaaaacca
    10021 ccagtagcag cagtaggtgt aaatgcactt ttaattatta acataaaagt ttcagggatt
    10081 ttattccaaa aagatattaa aatgattcca cagattacaa catatataac tgccataaaa
    10141 ggtactattt tagtagctac attagatata cttctaagtc ctccaaatac aaccaaagct
    10201 acaaatactg ctaatacaac ccctgtaatt ctagggtcta tcccgaaact attatttatt
    10261 gcatctgtta ttgaatttac ttgagtaaaa gtaccgattc ctaaaagtgc aactagtata
    10321 ccacttatag cgaaaaatat tgcaagaggt ctccattttt ctcccatacc atttactatg
    10381 taatacattg gcccaccaga cacttgacca tttttatctt tagttctgta ttttattgca
    10441 agaacacctt ctgaatactt agtagccatt ccaaagaatg ctgctatcca catccaaaaa
    10501 agtgcaccag gtccaccagc tttgatagct gtagcaacac caacaatatt accagtacca
    10561 actgtagcag ctagagcagt acataaggct gcaaaacttg aaacatcacc actacctttg
    10621 ttttcagcag agaatattaa cttaagagct cttggcagtt tagttatttg aacaactcca
    10681 agcttaacag taagtagtat accagtacca actaatagta ccagtagggg tggtccccaa
    10741 ataaaagcat caactttgtt taaaatatca attagattca taaaaacact ccctcatttt
    10801 ttttgcttta cataatcaat agaattttta atgaagtaat tgacaggaaa taagtataat
    10861 ttattaataa tttctagtgt aataaaaaaa gctaaatcta aaaaagattc agctctggat
    10921 gtacactaat tagcaaccaa aataaaagta ttagtaaata ctgtttttgg tagataatcc
    10981 ctgtcctttt gcctgagaga ttgagcataa aagccttgcc ccttcggcgc ccataatggg
    11041 tctctccaga gttccgtaaa ttttacagtc ctacttttaa aagtacctga gagtgttact
    11101 ccttcggtgt gctatatagc aactcttcta taaaatctta accggatatt gatttgtcat
    11161 agcttattgt attaataata ttaatataag tttttatgtt ttgtcaacta aaaaattaaa
    11221 aaacaaagaa aaaatataaa aatgtaagcc tatttacgaa ttataaactt agaaagatga
    11281 gtaatatacg taaaaatgaa ttttgtaata cacatacaaa tgaagtgtaa aataagtttt
    11341 aaataaaaaa ataataaaat cttaaataat taattagata tgattaaatt atggtaatat
    11401 tatagttacg atattaagaa aagttctatt tagttagatt ttaagcgtaa tatgagatta
    11461 tgtatagaga aaggatgtat ttttagatgg gaaaaatatt agtgctagca gaaaaacctt
    11521 ctgttggaag agatatagca aaagttttag ggtcaaaaaa tgaaaaaaat ggttatatag
    11581 aggggccaaa gtatgtgata acttgggcac taggtcattt ggttacactt gctgaccccg
    11641 aaagttatgg agaaagatat aaatcatgga gcttagaaga cctgccaata cttcccaagc
    11701 accttaagac tgttgtaata aaaaagagtg gaaagcagtt taatacagtt aaatcgcaaa
    11761 tgaatagaaa tgatatagat gaaatagtta tagcaacaga tgcaggtcgt gaaggagaac
    11821 ttgtagcaag gtggataata gaaaaatcac aggtaaaaaa acctataaaa cgtctttgga
    11881 tttcttcaag tacagacaaa gcaataaaag acggatttgc aaaattaaga agtggaaaag
    11941 aatatgaaaa tttgtattac tcagcaatag cacgagcaga agctgattgg attattggaa
    12001 taaatgcaac cagagctttg acaacaaagt acaatgcaca gctttcttgt ggaagagtac
    12061 aaactcctac tcttgcaatg ttgcttaaaa gagaagaaga aataagaaat ttcaaaccta
    12121 aagagtatta tggattagaa ttaattgcta caaagggaaa ttcagatata aaatttattt
    12181 ggaatgataa aaacaataat tcttcaactt ttagtaaaga aaagatagaa tccatattaa
    12241 aaaaagtaaa aggtgtagac ataaaaatat ctgaagtaaa caagtcatat aagaaaaaat
    12301 attcacctgc actctatgat ttaacagaat tacaaagaga tgcaaataaa atgtttggat
    12361 tttcagcaaa agaaacttta tctataatgc aaaaactgta tgaacatcat aaagtactta
    12421 cttatcctag aactgattct agatatttaa catcagatat tgttggaaca ttaaaagata
    12481 gaataaaagc tgtaaatatt agtgattatt caaagatttg taataaatta ttaaaaagca
    12541 atattatagc taataaatcg tttgttgaca attcaaaggt aacagaccac catgcaataa
    12601 taccaacaga acagaaggta ttcatgagtg attttagtga caaagagcga aaaatatata
    12661 atcttgtagt aaaaagattt ttagcagtac tatctcctcc ttttgaatat gagcagacaa
    12721 ctataagtgc aactgtagaa ggagaaatat ttaaggctaa gggaaacaaa gtaataaatt
    12781 taggttggaa agaaagttat aaagatattg aagatgaaga tgagtatgaa agtataccaa
    12841 atgttttaga aaaagatgta cttaaggtca agggattaaa gataacttca ggaaaaacaa
    12901 atccaccacc atacttaaat gagggaacat tattgactgc tatggaaaag aatggtctag
    12961 ggacagttgc tacaagagca gatataatag aaaaattatt caattcattt ttaatggaaa
    13021 agaaaaataa agagatatat ataacatcaa aaggaagaca attattagat ttagttccag
    13081 tagacttaaa gacaccagaa cttacagctt catgggaaaa gaaattattg gatatttcaa
    13141 agggaaaact tagtaaaaat acattcataa atgacatgaa agattattca aaagatattg
    13201 tactagaagt aaaaaatagt gataataaat ttaaacatga taacttaact aaaaatagat
    13261 gtccagaatg tggtaaattt atgttagagg taaatggtaa aaaaggtaag atgttgattt
    13321 gtgaagatag agagtgtaat actagaaaaa ctatatcaca agtaaccaat tcaagatgtc
    13381 ctaactgtca taaaaaatta gaacttcgtg gagaaggtga ggggagaatc ttcacttgta
    13441 gttgcggata cagagaaaag ttgtcttctt tcaacaagag aaagcaagaa gaaaaacaga
    13501 aggcatcaaa aaaagatgtt aatcaatatt taaaaaatca aaataaaaat caagagacat
    13561 ttaataatcc atttgcagaa gcattagcta agctaaaaaa caaataattt taaagttatt
    13621 attatacaca attataactt taactgtata ttagttatat gtactgatat aatatagtgt
    13681 atagtttagg catgaaagta gggctgttaa atggatttaa gtggcatatt taaatactat
    13741 tgtaaagagt gtgaaaatac atggaataat tcgagtgttg aattatttga ggatatagaa
    13801 acgtatagta aagattcaca aaaaaagagg gaaaaagaat tagataaatt tataaataca
    13861 atatcagttc atttagagag gtatccaagt gatgctgtat tgagaaaaat gtgggtaaaa
    13921 aagggcgagg ttttcttaca aaagacattg gaaaaagaaa atatttttaa gttagaaaaa
    13981 atggatgtag aggatagaaa aaaattttta gatataacaa aacagtttat tagagatgct
    14041 agaaaatttg atgatgattt acctataggt gatattatgc aagctatgag gaatgtatgg
    14101 atttcaaatg cattacaatt attatttggt aaagaaatat attattcaaa agctaacttt
    14161 gcatatagta tgttatatcc atatactgat aattatttag acaatacaaa tatagataaa
    14221 aatgataaga ttttatttaa taactggtta gaaaaaaggc tcctgggaga acatattaaa
    14281 tctaaggatt atcatgaaag taaagtatct caaatgatag attatattga aagtgtatac
    14341 cctagagaaa agtttacaca agtttatgaa tcgttattat taatatttaa aagtcaagta
    14401 aatagtttaa aacaacatgg taaggaaaat catttgtgta aagaagattt attatccatt
    14461 tctattgaaa aaggaggttc atccgtttta gtagatggat atttaataag tggatttatg
    14521 acaaaggaag aaatagagtt ttgtatagga tatggatttt tattacaaat atctgatgat
    14581 ttacaagata taaaagaaga tttaaaatac aaccataaaa ctattattac agagatgtca
    14641 aaagagggta ctttagataa agttgtaaat aaactaataa attttactat tgagttaata
    14701 gatagtttta aaattaataa taaaaataaa tctgtaataa ctatgataaa gaatgattgc
    14761 ttaatgttaa ttttattttc tgtagtttat aatgctgaat ttttttctgt aggatatata
    14821 a
//
LOCUS       DACTGW010000082        14816 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.082, whole genome shotgun sequence.
ACCESSION   DACTGW010000082 DACTGW010000000
VERSION     DACTGW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.082"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            3..79
                     /locus_tag="I9573_001945"
     tRNA            3..79
                     /locus_tag="I9573_001945"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Asp,seq:gtc)
     gene            90..164
                     /locus_tag="I9573_001946"
     tRNA            90..164
                     /locus_tag="I9573_001946"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:122..124,aa:Gly,seq:gcc)
     gene            174..247
                     /locus_tag="I9573_001947"
     tRNA            174..247
                     /locus_tag="I9573_001947"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Arg,seq:tct)
     gene            1223..2062
                     /locus_tag="I9573_001948"
     CDS             1223..2062
                     /locus_tag="I9573_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAT4796568.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            2059..3240
                     /locus_tag="I9573_001949"
     CDS             2059..3240
                     /locus_tag="I9573_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796569.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNIDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            3544..4446
                     /gene="ptb"
                     /locus_tag="I9573_001950"
     CDS             3544..4446
                     /gene="ptb"
                     /locus_tag="I9573_001950"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HAT4796570.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            4468..5547
                     /gene="buk"
                     /locus_tag="I9573_001951"
     CDS             4468..5547
                     /gene="buk"
                     /locus_tag="I9573_001951"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HAT4796571.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            5537..6214
                     /locus_tag="I9573_001952"
     CDS             5537..6214
                     /locus_tag="I9573_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT4796572.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            6257..6472
                     /locus_tag="I9573_001953"
     CDS             6257..6472
                     /locus_tag="I9573_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAT4796573.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            6501..7580
                     /locus_tag="I9573_001954"
     CDS             6501..7580
                     /locus_tag="I9573_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HAT4796574.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            7580..8332
                     /locus_tag="I9573_001955"
     CDS             7580..8332
                     /locus_tag="I9573_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HAT4796575.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            8333..8890
                     /locus_tag="I9573_001956"
     CDS             8333..8890
                     /locus_tag="I9573_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HAT4796576.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            9060..10406
                     /locus_tag="I9573_001957"
     CDS             9060..10406
                     /locus_tag="I9573_001957"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAT4796577.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILNGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            10673..12505
                     /gene="glmS"
                     /locus_tag="I9573_001958"
     CDS             10673..12505
                     /gene="glmS"
                     /locus_tag="I9573_001958"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAT4796578.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEEEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            12958..14253
                     /locus_tag="I9573_001959"
     CDS             12958..14253
                     /locus_tag="I9573_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HAT4796579.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKMQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKRAVDFSANWLNVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSITPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSNSVSLEEYRNIDNTMRNKSEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
BASE COUNT         5852 a         1636 c         2887 g         4441 t
ORIGIN      
        1 gcggcctggt agttcagttg gttagaatgc cagcctgtca cgctggaggt cgagggttcg
       61 agccccttcc aggtcgccaa attaaataag cgggaatagt tcagtggtag agcgcaacct
      121 tgccaaggtt gaagtcgcga gttcgaatct cgtttcccgc tccataataa cttgggtgca
      181 tagctcagct ggatagagca acgcccttct aaggcgtgtg tccggggttc gaatccctgt
      241 gcgctcatta agaaaagttt actataaatt atagtaaact tttatattgt ttaaaaagac
      301 acaggaaaca tgtatattac ctgtgtcttt ttatttataa actttaaact atgtaaaaat
      361 tacagtatat tctaatagag ttacagtata ttcattaata ttgtaaacat acggagggta
      421 atgtataata gtctacaaga tgtttatgaa ggcatcagtt aaatataact aattagataa
      481 gagttatata cattatattg tatactttat ggtatttata tattgaaata acttatgaaa
      541 ttatttataa agagatttat gagcatattt ttgtctaaac aataagtata agaaattata
      601 aaaaattaga gactatagtg aattgacaat ttaatgtaat atttataagt tatactaagt
      661 aaattattat actgtgaaat ggatatataa ctttaggtat tgtgtattat tttcactgta
      721 ggagattata tctagttata cataataagt tttaatgata gtttatagta agatattaaa
      781 ataaatttat ctaatctaga tatgaaataa gttattactt taatataaaa atgaatctaa
      841 tggaataata ttgatgataa gtaatattta gattttgtat ttgtttatag ttaataagtg
      901 gattatagaa tttataataa ttaatatata ttaaacaaag atatattaag atattttaga
      961 acataaaact gcattaaggc gcgagtacta ttttgtattc gcgccttgat ggtttgagtt
     1021 atttgagtaa aaactaactt taataagaaa tatgtattta ttaagttgat atttttaatg
     1081 tgtttagaga ttatttaaca atctccaaat ataaaactag aattatttac ataaaagtta
     1141 aatagtttgt atagagttag atttatatag tataatactt agtgtaaata attaagaagt
     1201 tatcaaacag gaggataaaa ctgtgttaga tataaattct tttttaaatg gattttttaa
     1261 tataatatta agaatgagta tatatgattt aattgacata tctatagtag cgtatatttt
     1321 ttacaaaata tttatgttta taaaagatac aagagcagaa caagtgttta aaggtataat
     1381 atttctttta ttagcaactc aacttagtaa cacctttaaa ttgcataccg tttattggat
     1441 atctcttaaa gctttagatt atggagttat agctgctctt ataatttttc aaccagaatt
     1501 tagagcagga cttgaacata taggaagagc aaagtttaat ttatttggaa agaatgtaaa
     1561 tacttctgaa gaaactctca atagaaatat agaagaaatt gtagaagctt tatattcact
     1621 ttctagacaa aaaataggag ctcttatcat aatggagaga gaaactagaa taagtgatat
     1681 aataaataca ggaactatta ttgatgctga aatttctagg caactattaa taaatatatt
     1741 tataccaaat acaccattac acgatggagc tgttgtaatt agagattcta aggtaaaagc
     1801 agctgcatgt tttttgccat tgacggaaag taaggattta agtaaagatt tgggtactag
     1861 acatagagca ggaatagggg taagtgaggt gtctgactgt ataactctga ttgtatcaga
     1921 ggaaacaggt ggcgtatcaa ttgctaaagc aggtaagtta tatagagata tttctagaga
     1981 aagaatgatg aatatattac gtagtaattt aaaaacaaat acagaaacaa gaagtttttt
     2041 caaaggtggt atatttaaat gataaataga ctaaaaaata ataccaaaat aaaaataata
     2101 tcatttttaa gtgcaattgc attatggatg tatgttatgg ctgtagttga ccctgaagaa
     2161 actaaagggt ttgaagatat acctgtatct ataagtaata tgagtgattt aaaggataaa
     2221 aatcttgtta tatatccaga cgaaaaatta acagcagatg tatatataaa aggtagttta
     2281 tcaaaggtac aaagtgttaa gaaagaagat atacatgtat atggaaccat tgaagaccca
     2341 atacaaggac agaaggaagt atatttaaaa gcaaatattc ctcaaggtgt tactctagaa
     2401 tttaagcatg atgttttagt agtaaatctt gaaaaaaaca taaaagaaaa aagagatata
     2461 aaggtagaag taagtggaaa ttcaaaaaga aatataaata aaattgaact tagtaaggaa
     2521 tttgtcgatg tatcaggtcc aagaactcta gttaaagaag tcaaaagtgt aagagctaat
     2581 ctagatgttg gtaatgagac agatgatttt tctaaaaaat tgcaattaat accattaaat
     2641 agtgatggaa ctaaggttga aggagtaaca ttaagttcat cctttgtgac tgcaaagata
     2701 gaactattga aggaaaaaat tgtcccaata agagtaagtc taaatgataa taaagataat
     2761 gatgtactga aaaactataa attaagtaag gaacaagtta caataaaagg aaagaaagaa
     2821 gatatagaca aaataagtta tattaagaca aaacctattg acttagctga tttggctaat
     2881 ggtgaagaaa aagaagttag cttagaaatt ccagagggta taactcttga ggatagctct
     2941 ataacaatta aattaaatga tacaaaacaa ttgagtgcag aatttttata taatgcggga
     3001 gatattgaat taagaaatat cccaacaaat atagataatc caaatgtaat ttctgatgta
     3061 gatataaaag ttgttgtaga gtcaacggag gatttgagta agttaaataa aaaagatatt
     3121 tcattatata ttgatttatc acaaggccct gaggaagata gtaaatataa aataaaatat
     3181 gaaacaacat atcaatttaa aaaaataaat atagaaccta atattgttga agtggaatga
     3241 acaaatattt cgaatattta gttgttacaa gatgcgacaa aaaaacctat aaaattaagt
     3301 ttgttaattt tatgtttttg ttgcatcttg tttattttag ttatattata aaataaggac
     3361 aatgagatat ttgttaaatc taaaaagcgc tagatatatg aaaaaagact gataaataga
     3421 tttaaataaa tattttgaat taaatatttc aattatctct aggaaaacgt tgctgaacga
     3481 gttataattt aattgagata taaaaagttc tatttcaaaa aattcaagat gaggtggaaa
     3541 tgcatgagaa gttttgaaga agtaattaag tttgcaaaag aaagaggacc taaaactata
     3601 tcagtagcat gttgccaaga taaagaagtt ttaatggcag ttgaaatggc tagaaaagaa
     3661 aaaatagcaa atgccatttt agtaggagat atagaaaaga ctaaagaaat tgcaaaaagc
     3721 atagacatgg atatcgaaaa ttatgaactg atagatataa aagatttagc agaagcatct
     3781 ctaaaatctg ttgaattagt ttcacaagga aaagccgaca tggtaatgaa aggcttagta
     3841 gacacatcaa taatactaaa agcagtttta aataaagaag taggtcttag aactggaaat
     3901 gtattaagtc acgtagcagt atttgatgta gagggatatg atagattatt tttcgtaact
     3961 gacgcagcta tgaacttagc tcctgataca aatactaaaa agcaaatcat agaaaatgct
     4021 tgcacagtag cacattcatt agatataagt gaaccaaaag ttgctgcaat atgcgcaaaa
     4081 gaaaaagtaa atccaaaaat gaaagataca gttgaagcta aagaactaga agaaatgtat
     4141 gaaagaggag aaatcaaagg ttgtatggtt ggtggacctt ttgcaattga taatgcagta
     4201 tctttagaag cagctaaaca taaaggtata aatcatcctg tagcaggaag agctgatata
     4261 ttattagccc cagatattga aggtggtaac atattatata aagctttagt attcttctca
     4321 aaatcaaaaa atgcaggagt tatagttggg gctaaagcac caataatatt aacttctaga
     4381 gcagacagtg aagaaactaa actaaactca atagctttag gtgttttaat ggcagcaaag
     4441 gcataatttt aagggggaaa taggcaaatg agcaaaatat ttaaaatctt aacaataaat
     4501 cctggttcga catcaactaa aatagctgta tttgataatg aggatttagt atttgaaaaa
     4561 actttaagac attcttcaga agaaatagga aaatatgaga aggtgtctga ccaatttgaa
     4621 tttcgtaaac aagtaataga agaagctcta aaagaaggtg gagtaaaaac atctgaatta
     4681 gatgctgtag taggtagagg aggacttctt aaacctataa aaggtggtac ttattcagta
     4741 agtgctgcta tgattgaaga tttaaaagtg ggagttttag gagaacacgc ttcaaatcta
     4801 ggtggaataa tagcaaaaca aataggtgaa gaagtaaatg ttccttcata catagtagac
     4861 cctgttgttg tagatgaatt agaagatgtt gctagaattt ctggtatgcc tgaaataagt
     4921 agagcaagtg tagtacatgc tttaaatcaa aaggcaatag caagaagata tgctagagaa
     4981 ataaacaaga aatatgaaga tataaatctt atagttgcac acatgggtgg aggagtttct
     5041 gttggagctc ataaaaatgg taaaatagta gatgttgcaa acgcattaga tggagaagga
     5101 cctttctctc cagaaagaag tggtggacta ccagtaggtg cattagtaaa aatgtgcttt
     5161 agtggaaaat atactcaaga tgaaattaaa aagaaaataa aaggtaatgg cggattagtt
     5221 gcatacttaa acactaatga tgctagagaa gttgaagaaa gaattgaagc tggcgatgaa
     5281 aaagctaaat tagtatatga agctatggca tatcaaatct ctaaagaaat aggagctagt
     5341 gctgcagttc ttaagggaga tgtaaaagca atattattaa ctggtggaat cgcatattca
     5401 aaaatgttta cagaaatgat tgcagataga gttaaattta tagcagatgt aaaagtttat
     5461 ccaggtgaag atgaaatgat tgcattagct caaggtggac ttagagtttt aactggtgaa
     5521 gaagaggctc aagtttatga taactaatga taaaagaata agaatcataa ctggtcacta
     5581 tggaagtggt aaaagtgaat ttgcgatgaa ctatgttgtt aagttaagag atatggtttc
     5641 tggcaaagtt gctatagccg atttagatgt ggtaaatgta tactttagaa ctagagaaaa
     5701 gaaagagtta atgaaatcct taggtataca accaatagat agctcaatta atgctccaac
     5761 attagattta ccagcagtat cagcagaggt aatgtctcct atggtagacc attcatacaa
     5821 ttctgttata gatttaggtg gagataatgt aggtgctagg gttataggaa gatttagtca
     5881 tttgttaaaa gaaggcgatt atgatatgct ttttgtaata aatgcaaata gggagaaaac
     5941 tcaaacttct gaagaagtta tccaatatat aaaagaaata gaaaaatctt ctaaattaaa
     6001 ggtaacaggt ctaattaata atactcatct tattaggttt actactattg atgatgtttt
     6061 aagaggtcag aaagttgcaa aagaagtttc tgaaaaatgt aatataccga taagatatgt
     6121 tgcatgttta gaaaatcttg ttgaacagtt accaaaagat ttagaaggag aaatttttcc
     6181 tataaaatta tatatgagag aagactggat gtagtttgag ctcaaatata tagaaagttt
     6241 aaggaggctt aggtaaatgg ctaaaggaaa agtatctttt aatcaagaac gttgtaaagg
     6301 ttgtggattg tgtgttgaag catgtccagt aaaaatcata caattagact caaatgtaat
     6361 aaacaaaaaa gggtataacc cagcaactgt ttttgaaatg gaaaaatgca taggatgtgc
     6421 aagttgtgca actatgtgtc cagatgtagt tataacagta gaaagagatt aatttaatta
     6481 atatttaagg gggaatttta atggctaaga tacttatgaa aggtaacgaa gcatttggaa
     6541 aagcagctat agaagctggt tgcaaatatt tcttcggtta cccaataact ccacaaagtg
     6601 aattaccaga atatttatca agagagttac ctaaaatagg tggagcgttc gttcaagctg
     6661 aatcagaagt ttctgcgata aacatggttt atggtggagc aggtgcagga gcaagagtta
     6721 tgacttcttc atcttcacca ggagttgctt taaagcaaga aggtataaca tatgcagtag
     6781 gagcagaagt tccttgtgtt gtacttaatg taatgagagg tggtccagga cttggaagta
     6841 tacaaccttc acaagctgac tactttatgt ctacaagagg tggaggaaat ggagactaca
     6901 gaactccagt attcgcacca gctacagttc aagaagctgt tgatatgata atggaagctt
     6961 ttgacgttgc agattattac agatcaccag ttatggtagt tgcagatggt atgataggtc
     7021 aaatgatgga gccagttgaa tttagagctc ctgagaagaa aagagaattg cctcctaaag
     7081 attgggcaac tgttggaact aaaggaaaaa gaaaacctaa tgtaataaac tctctatact
     7141 tagaaccaga agtactagaa gaccattgtt ggcatttaca agaaaaattt gatgcaatgg
     7201 aaaagaatga agtacaatac gaaatgtaca aaacagaaga tgctgaattt gtatttgcag
     7261 cttatggaac tacttcaaga gtagtaaaaa gcgcaattga tatattaaga gaagaaggaa
     7321 taaaagcagg tcttataaga cctaaagtat tatggccatt cccattcgaa gcattcaacc
     7381 aaattcctaa tgctagaaac atattgactg ttgaaatgag tatgggacaa atggtggaag
     7441 atgttaaaat ggcagtagaa ggtaaattac cagtttactt ccatggaaga ccaggaggaa
     7501 tgactccaac accagcagaa atagttgaaa aagctaaaaa aattattgcc ggagaattgg
     7561 ttgcaggagg tgctagataa tggctgttgt atttaagaaa actgaaggat tacaagatac
     7621 tcaaactcac tactgtccag gatgtacaca tggtataatc catagattag ttggtgaagt
     7681 attagaagaa ctaggagtat taggtgatgc tgtaggtgtt gttccagttg gatgttcagt
     7741 tttaggatat aaatatttta actgtgacac tcaagaagct gctcatggta gagcgccagc
     7801 tgctgctaca ggaataaaaa gagttcatcc tgaaaataca gtattcactt atcaaggtga
     7861 tggagactta gcttcaattg gtactgctga aatagttcat gcagctgcta gaggagaaaa
     7921 aataactact atatttgtaa acaatacaac ttatggtatg actggtggac aaatggctcc
     7981 aactacatta gttggacaaa gagctactac tgctcaatca ggaagaaatg ctgaaactca
     8041 aggttatcca ataagagtaa gtgaaatgtt agctacatta acaggagctg tatttgtaga
     8101 aagagtagca gttgatactc cagctcatgt tagacaagct aaaaaagcta taaagaaagc
     8161 attccaagtt caacaagcag gattaggatt tggaatagta gaagtattat ctacttgtcc
     8221 tacaaactgg ggactagctc caaacgatgc tttacaatgg ttaagagaca acatgatacc
     8281 atactatcca ttaggaaatt tcaaaaatgt tgaagttgag gaggtgaagt aaatgtctac
     8341 agctagagta atatgtgcag gcttcggagg ccaaggtgtt atgtctatgg gacaattact
     8401 tacttatgca ggaatgctag aaggaaaaga agtttcttgg ttaccatctt atggaccaga
     8461 aatgcgtgga ggtacagcaa actgtgcagt aactgtatct aatgagccag ttggttctcc
     8521 acttataaca gatgatgcaa ctgcagctat attattaaat ataccagcat ttgaaaaatt
     8581 caaagatgat gttattcctg gtggaaaaat aatagtaaat agttctttaa taaaagaaaa
     8641 agttgataga acagatgtag atgtttatta tataccagca aatgaattag cagctgaatt
     8701 aggaaatgat aaagttgcta atatgataat gttaggcgct tacttaaaag tatctgatac
     8761 tgtagatata gaatctgtac tagaagcatt caaaaaagtt ttcggaccta gaaaagaaaa
     8821 attcgttcca ttaaacagag aagctttaca aaaaggtatg gatgctacat gttctaaagc
     8881 aactaactaa taaagaaaaa gatgtcaaaa aaaagacaat agaattattt ctattgtctt
     8941 ttttgctgat aagtattaaa atgtttttat gactgaaatt aagcctataa tgatatttta
     9001 tgaactataa aaatataata caaattacaa gcttttcaat tatgaaagga gaatctttag
     9061 tgagaaagta ttttggaaca gatggagtta gaggagtagc aaatacagaa ctaacatgtg
     9121 atttagcata taaacttggt agagctggtg gatttgtctt agctcaagga gaccacagag
     9181 taaaagtagt agtaggtaag gatactagaa tatcaggtga tatgttggaa gcatctttaa
     9241 tagcaggact tatgtctgtt ggatgtgatg ttattactgt tggaataata ccaacacctg
     9301 cagtggcata tttaactaga aaatatggtg ctgattgtgg tgtggttata tctgcatctc
     9361 acaatcctgt tgaatataat ggaatcaaat tctttaataa gaatggatat aaattagatg
     9421 atgaaattga attgaaaata gaagaatata tagacgatat agacaaaatt gattgtcttc
     9481 caattggtga aaatgtagga agaaaattac atgaacattg tgcacaaaga gactatgtag
     9541 actacctaaa atcaataata agcactgact ttaagggatt aaaagtagtt ttggactgtg
     9601 ctaatggtgc gtcatataaa gtggcaccaa tagtgtttga tgaactagga gcaagtgtaa
     9661 tttcaataaa tagttcacca gatggaaata atataaacta taaatgtggc tctacacatc
     9721 cagaacaact tcaaagggct gttttggaac ataatgctga cttaggtctt gcatatgatg
     9781 gagatgctga tagattaatt gcagtaaatg aaaagggtca aattgttgat ggagaccata
     9841 taatgatatt gagtgcctta aatttaaaga aaaataataa attggcgcaa gatactttgg
     9901 tagttacagt tatgagtaat atagggttga caatagctgc taaggaaaat ggaataaatt
     9961 tatctactac agcagttgga gacagatatg ttttagaaga tatggttaaa aatggatata
    10021 atcttggtgg agagcaatct ggacatatga tatttttaga ttacaacact actggagatg
    10081 gagtgcttag ttctctaata cttgcaaata taatcttgca agaaaagaaa cctttatcag
    10141 aaattgcatc aataatgagc caatacccac aagtattagt aaatgcaaca ataaaaaatg
    10201 aaaataagaa caaatatatg gaatatccag aaataaaaac tgagatagaa agaatagaat
    10261 ctattttaaa cggaaatggt agagttttga taagaccatc aggaactgag ccattagtta
    10321 gagttatgtt agagggtaaa gaggaaggtc aaataaaaga attagcaaca aacttagcta
    10381 atcttataca agaaaaatta tcgtaattta aagggtattc tcagatacaa atactgagga
    10441 cacctttcta agcgccagaa cttagttata gacataatta ttgagtaata taggaactaa
    10501 gttgacgagg tcagagttaa tcgaattgtc ggcggatgct ctgcggtgtg tttaccatcg
    10561 aaagacttct ttaaataata ggagcaatcc tatggacaaa gggaagccaa agtaaactta
    10621 tatgacctaa ctcttaaaat atataaataa tatttttagg aggacacaaa taatgtgtgg
    10681 aatagttgga tatttaggaa gtagaaaggc agcagaagtt atagtagaag ggctttctaa
    10741 gctagagtac agaggttatg actcagcagg agtagcagtt aatagttcta atgaagaaga
    10801 acttaatatt agaaagttta aaggtagact ttctgtactt gcagaagact tagaaaagaa
    10861 tcctattgat ggaaatttag gtatagggca tacgagatgg gcaactcatg gagaaccatc
    10921 tgatgtaaac tcacatcctc attttaatca agccaaaaca atagcagttg ttcataatgg
    10981 gataatagaa aactatatgg aaataaaaga agaattaatt tctgaaggtg ttaagtttga
    11041 atctcaaaca gatacagagg taatagcaca tttagtagat aaatactatg agggtaattt
    11101 attagatgct gtatacaaaa ctatatcaaa attaagagga gcttatgcat taggtgttat
    11161 atgtaaagaa catggaaatg aattagttgc agtaagaaaa gatagtcctc ttgtagttgg
    11221 tgttggtgaa ggtgaaaact ttatagcatc agatatacct gcattattaa aatatactag
    11281 agatgtttat ttcttagaaa atggagaagt agttcatcta aaagacgaaa atgtaacagt
    11341 ttatgactct aatagaaatc ttgttgaaaa agaagtattt catgtaactt gggatgtaga
    11401 agctgcgtct aaaggtggat atgactactt tatgtcaaaa gaaattcatg agcaaccaac
    11461 aggagttaga gaaactttag aaagaagact tgatgataat ggaaatataa ttctagacag
    11521 tataaatatt tctaaagaag acttagaaaa aataaataaa gtttatatag tggcttgtgg
    11581 aacagcatat aatgctggat tacttggtaa gtatgccata gaaaaatttg taaatatacc
    11641 agtaataact gatatagcat cagaatttag atatagcgac ccatttgtag atgaaaattc
    11701 attagtaata ttagttagcc aatctggtga gacagcagat actctagcag tattaagaga
    11761 ttcaaaggct aaaggagcaa gaatactttc aataactaac gttgtaggtt catctatagc
    11821 aagagaatca gatgatgtat tctatacttg ggcaggacct gaagtagcag ttgcatcaac
    11881 taaggcatat actactcaaa taacttcttt atacatgata gctttagact ttgctatcaa
    11941 aaaaggaaca ataactagag aattttatga tagtatgatt tctaagatga aagaaatacc
    12001 atcaaaaata caagaaatat tagacaatga agaatatata aaagaagttg ctaaaacagt
    12061 tgttagttct gaacatgcat tctatttagg aagaggtata gactatagct tagcaatgga
    12121 aggttcattg aaattaaaag agatttcata tatacatgca gaagcttttg cagcaggaga
    12181 gctaaagcat ggaacaatag ctttaataga aaaaggaact cctgtaattg ccatagctac
    12241 tcaagaaaag ttatttgaaa aaatggtttc taatatggaa gaagtaagag caagaggagc
    12301 ttatgtagta gcaatagctc aaagccataa taaggatgtt gagaaagctg ctgataaaat
    12361 aatctatata ccaaattcag atgatatttt atcaccaata ttagcagttg taccaatgca
    12421 attacttgcg tatcatgtct ctgtactaag aggatgcgat gtagataagc caagaaatct
    12481 agctaaatca gtaacagttg aataaaaaca gattaaaaat taaatagata atatttattg
    12541 aaaaaaactc atcctgttat ttgtatttta taggatgagt tttttagata aatattaaat
    12601 ttgttttgtt tttgctataa taaattttat aaaaagattg atattaaatc atctgtaaaa
    12661 tgataaatat atagaccata ccaggtatag ggaacgataa atatagttca tataaaatat
    12721 agtgagtact taataaacat atagtcaata tcagacataa aaaaataata gcttatgaga
    12781 aaaattaaaa tacaaggtat tagtaacctt gtgataagat aaagtgagta gtttaaaata
    12841 agaatataag gaactattta taaaagaata taatgaagct aatagagtat aagaaaatta
    12901 ctttatttta gtaaaataat ttcaaataaa aattttttta aattgggagt ataaattatg
    12961 acaaatgatt ttaaaaaaga aaagaaagaa gattattttg ttaatcttaa ggcaatatct
    13021 actgaagttt tacaagaaaa aatgcaagat aacgcttggg ttatagttga tactcgctta
    13081 aatgatgctt ataatggctg gaagttagat ggagtaaaaa gaggtgggca tataaaaaga
    13141 gcagtagatt tttctgctaa ttggttaaat gtatactcag atagaaaaga tgaagtgcta
    13201 gaacaagcat tgaaaactaa aagaattgat ttagataaaa atatagtatt gtatgatgca
    13261 aatggaaaag atgctttggt tgtagcagat tatttatcta aaaaaggata taaatatcta
    13321 tataaatatg atattaaaca atgggcagac gatgaaaatc ttccaatgga aagatataaa
    13381 aattatcaaa tgatagttcc tgcttttata ataaaagata tattagatgg aaaaattcct
    13441 gagacatttg aagattcaaa gaatataaaa atgattgaag cgagttgggg agaagaatcg
    13501 tatacaaaag ggcatatacc tacaagtgtt catgtaaata ctgatattat agaacctcct
    13561 ccaacatgga tgcttgataa tgatgataac ttaactaagt ttgctttaga ctatggacta
    13621 acaaaggatg atactgtaat agtttcaagt attacaccta tggcatctta tagactagct
    13681 gttatattga gatatatagg agtaaaagat gtaagagttt tgaatggtgg aacaaactca
    13741 tggttatcag caggatatga attagaattt attagtaatc caaaacattc atgtactaat
    13801 tttggagcag atataccagt taactcacaa ttagttgtta ctacatctga attaagacaa
    13861 aaactaaaag aaaaaaataa gtttatacta gtagataatc gtacttggga tgaacatata
    13921 ggaaaagtat ctggatatac atattatgat aaaaaagggc gtataccagg agctttatat
    13981 ggacattcag gtagtaatag tgtttcacta gaagaatatc gtaatattga taatacaatg
    14041 agaaataaat ctgagatttt agaaatgtgg gacaaggaaa atatagatgt gaataaacag
    14101 cttatattta tgtgtggaag tggatggcgt gctgctgaag tattaacata tgcaaatgtc
    14161 ataggagtgg aaaatacctc cctttatagt gatggatgga tgggctggag tcttgataat
    14221 tcaaatttaa ttgaagtggg agaacacaaa taaagttttt gaaagtacaa tagagttgtt
    14281 taaaacaaag gtgtttttga tttattagga agagtgtgta aattgtgtat tgggaagtgt
    14341 acttaggtat gttcatttgg agctatggaa attgataaaa aagtattatc tgtttatttt
    14401 gctggtaaaa tatgaataga ttatagatta tagattatag attatagatt atagattata
    14461 gattatagat tatagattat agatttaagt atagactaag taatttatat tacagaagat
    14521 aatatataag aattaatgca agatgtattt gatacatata aggaattggc aagtccagga
    14581 gaaggataga gaaaattgag attgaagagt ttgaaagaga ttattagatg aaaaatacaa
    14641 aaatgtataa aaatacatat tgagaaagta tatctctctt aaatttgtta ataattctat
    14701 tatacagaaa aacatattta agggggattt tttactatga aaaaagtaaa attagctata
    14761 agctttttta tattaatgat tataggaatg acaatatctt atgcagatac aagtat
//
LOCUS       DACTGW010000083        14576 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.083, whole genome shotgun sequence.
ACCESSION   DACTGW010000083 DACTGW010000000
VERSION     DACTGW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14576
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.083"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..264)
                     /locus_tag="I9573_001960"
     CDS             complement(<1..264)
                     /locus_tag="I9573_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796580.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGI"
     gene            complement(257..646)
                     /locus_tag="I9573_001961"
     CDS             complement(257..646)
                     /locus_tag="I9573_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4796581.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            838..1284
                     /locus_tag="I9573_001962"
     CDS             838..1284
                     /locus_tag="I9573_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796582.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEDIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            1548..2273
                     /locus_tag="I9573_001963"
     CDS             1548..2273
                     /locus_tag="I9573_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4796583.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCKYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            2260..3504
                     /locus_tag="I9573_001964"
     CDS             2260..3504
                     /locus_tag="I9573_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4796584.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMKNIDYGQKILSKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            3584..4261
                     /locus_tag="I9573_001965"
     CDS             3584..4261
                     /locus_tag="I9573_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796585.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            4262..6823
                     /locus_tag="I9573_001966"
     CDS             4262..6823
                     /locus_tag="I9573_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4796586.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDQNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIKKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGESYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGFGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNDFNFYDEISSSESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            7053..8318
                     /locus_tag="I9573_001967"
     CDS             7053..8318
                     /locus_tag="I9573_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAT4796587.1"
                     /translation="MSHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQEAK
                     LVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPPMAG
                     FFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLTNDN
                     EVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSLINN
                     KFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFGNLH
                     PVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVCVRN
                     CHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAILSAI
                     LKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(8479..11520)
                     /locus_tag="I9573_001968"
     CDS             complement(8479..11520)
                     /locus_tag="I9573_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HAT4796588.1"
                     /translation="MFKQRISKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGNTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            11727..12026
                     /locus_tag="I9573_001969"
     CDS             11727..12026
                     /locus_tag="I9573_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAT4796589.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QDLINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(12264..14483)
                     /locus_tag="I9573_001970"
     CDS             complement(12264..14483)
                     /locus_tag="I9573_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAT4796590.1"
                     /translation="MKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRGNYA
                     VLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGDQLI
                     EQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEIIYT
                     VNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLTKVE
                     NSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDLKKF
                     LEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLSEKL
                     SNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVISGQ
                     QPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLDKAE
                     AKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMKKLV
                     SLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALLSTS
                     VHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTSMVF
                     NFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYAIGA
                     TREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLTGH"
BASE COUNT         5543 a         1764 c         2176 g         5093 t
ORIGIN      
        1 aataccaata aaatatgaaa gttccaattt attaaatgta aaaaccagaa taaaaactaa
       61 tataaagtct ataataaaac ctaaagataa tagtttttta aattctttat ctaattttat
      121 aaagtaatta taatttttat gctcttggtc tatagaaact ccatagatgt gttttttacc
      181 tacaaaatag ggcataaaat aaaatgtagc atacatcaaa attagaactg gaaatattgt
      241 aaatagacat tcaatcttac tcataatatt tcacctcata cttgtcaaat aaacttgaac
      301 ttagtgacaa aaattcttct ttactaatac ctgataaata agattttgcc accaataatt
      361 ccaagtcaag tttatttttt tgacgagttg tattatctat tttcattggt agttgagcta
      421 caatagcacc ttttcttcta tcaatattta agaatccctc ttcttttaac tcattgtagg
      481 atttgttcac tgtatgtaaa tttacaccaa tattttctgc catacttctt acagatggaa
      541 gtgattcatt tattttcaat tctcctgaag ctatagcctt agttatttct tctttaattt
      601 gtacataaat agatttatca ctattaaaat ctaaatttaa aaccataaaa aacctccatt
      661 atgctttata aatgaaaacg attgttatat acatagtata acataaaaaa taataacaaa
      721 cacaaaatat aagtttatat tttgtaaaaa ttgtaattta aaaaataaca taatttttat
      781 taaaataata cagatactat ttatagatag aaataaaaat tagaaaggag aaaaaatatg
      841 ttaacatatt taatgaaaag acaaatgcat aaaaaagatg atggaatgag taaaccaatg
      901 atgattacag gagctgtaat tacaggtgta ggagcatatg ctgtatacaa aatggtaaaa
      961 agccataaat ctaattctca aatggtcaat acaaattatt atggaaatag cgattatgat
     1021 gattataaat atgacgaatt tgattatgac tgtgattgta gagacacaaa acaagattat
     1081 gaatatgaag aattaaaaaa aaaagtaaaa gaatttaatt caagaagaat aaattgtgaa
     1141 aattgtccac atgtttctca agaagaaatg atgcaatatg taaatagtgt taaagatgta
     1201 aaggaagata ttgatttaca tgaagatgct aaaaaagata atatttacaa agaagatgaa
     1261 tacaaaaaat atgaagaaga ataagtaata ttttgtataa ctgaacaaat agtaatgtca
     1321 aattatttaa tatctagttt taatatgtaa gttgtaactt aacttgaaag aatcagtatc
     1381 attctttaga tagttaagtt acaacttaca ttttgttttt ataaatttta atagttcttt
     1441 gtgactattt tgtaatttta gaaatatttt agtatccagg gtaattgtgt ataataaaac
     1501 ttgtatagat agatagtatg aaattaagca gatagtttaa aattaaaatg aaaagaaagg
     1561 aaatagttat gttaaagttg ttgatagttg aagatgatag tactatatct tttggaataa
     1621 agtatgcttt agaacaagaa ggatttagta tagatatatc aaaggattta tctagtggaa
     1681 aagaaatgat ttcttctaat gaatatagca tgatactgtt ggatgttaca ttgccagatg
     1741 gaactggtta tgagctttgt aaatatataa gaggattctc tcaggttcct ataatattct
     1801 tgactgcttg tgatgaagaa gtaaatattg taatgggtct cgatataggt ggagatgatt
     1861 atataacaaa accttttaga attagagaat taatatccag aataaaagct gttttaagaa
     1921 gaaaaggtaa tacttcagaa gaaaataaga aaatacttaa atttggagat ttaagtatat
     1981 atacattaga agctagagta tataaaaatg ataaagaaat atttctgaca tcagttgaat
     2041 ataaacttct tttaattctt atacagaata aaaatactgt tttaagtaga actcaaatat
     2101 tggaaaaact ttgggatgta acttatgatt ttgtaaacga caatactcta actgtttata
     2161 taaaaaggct tagagaaaaa ataggtgatg atttatgtga accaatctac atagaaactg
     2221 taagggggat tggttataaa tggataggaa gtgaaaatag tgtttctata taatccagaa
     2281 gtgaagaagt ttttaagtaa gtatgttact ttgatgttta ttgttatcat aatatctatt
     2341 ggatttagca taatcaacgt gtctttaact aaagagatgg ttgtaagaaa taatcaagct
     2401 ataataggga ctttatcgag taagtatcca aacttagaaa gtcagatagt agacataata
     2461 actcaaggga agtctatgaa gaacatagat tatggtcaga aaatattaag taagtataat
     2521 tatgataaat ctattaagat aaactctgag cctattattt ctaaattagt tttagagact
     2581 atcaaaataa atataatatt agtatgtata atatttattt tgatatttgt gttggttgtt
     2641 agatatttta cgtcaattta caatgattta tcagatatga ctaagtatgt ttactatagt
     2701 tcagagggta agccatttga tatgaaaaac aagaatcaag aagggcagat aggtcttcta
     2761 aaaactgaac tcttaaagat gacgacaatt cttaatgaaa aggttgaact tttaaaaaga
     2821 gaaaagatat ttttaaacaa tacaatttcc gatatatcac atcaattaaa aactcctatg
     2881 acttctttaa taatgttgaa tgatttatta tataatgatg taccatacga agtaaaaatc
     2941 gattttttaa ataagataaa aaatcaatta aatagaatgg attggttgat taaaagcatg
     3001 cttaaattat caaaggtaga agcaaaagtg ataaatttta aaaaagataa ggtaaaattt
     3061 agtgaactca tacatagagc aatgctatct atgaaaatac ctatggagat taaaaatcaa
     3121 aaactgacta tagagggaaa tgataatgta tcttatattg gtgatattga ttggtctgtg
     3181 gaagcactag tgaatataat caaaaattgt gtagaacata ctcctgaatt tggaaatata
     3241 attataacct ataaagaaaa tccattattt tctgaactaa taataaaaga tgagggtgaa
     3301 ggtatagata aaaaagatat accacatgta tttaaacgat tctatagagg aagaagtagt
     3361 tcaaaagaag atagtgtagg aataggtctt gcaatgtcaa aatctataat agaaagccaa
     3421 aatggcgata tatatgtaaa cagcgaaaaa ggtaagggta cagaatttca tataatattt
     3481 cataaaatgt atgatagtga ctaatctgta atatttaatt cactttatag taatagccaa
     3541 aacctaatat atgaagtata gaatataaat attggaggta gttatggaaa ttttaagagt
     3601 agaaaatcta actaaaagtt atggtaaaaa tgaaacaaaa gttgatgcta taaaaaatgt
     3661 aagtttatca gttgaaaaag gaacgtttat agcaataaca ggtccaagtg gaagtggtaa
     3721 aagtacctta ttacatttat taggaggagt tgacagacca actagtggta aggtttatat
     3781 aaatgatgtt gatatatata atttaaaaac taaggatttg gcgatattta gaagaagaaa
     3841 tataggtctt atatatcaat tttacaactt aattccagtc ttaaatgtga aggaaaacat
     3901 attgcttcca gcagagcttg acaatagaga tattgataga gaatattttg atgatttaat
     3961 gaaaacatta ggtctaattg atagagaaaa gcaccttcca aaccaattat caggaggtca
     4021 acaacagaga acctctatag gacgtgcatt aattaataga ccttctataa tattagcaga
     4081 tgagccaaca ggtaatttag atagtaaaaa ttcaaaggaa atattagaat tgttaaaatt
     4141 gtcagtaaaa aaatacaatc agacactaat aatgataact catgacaaaa atatggcact
     4201 tcaagctgat agaattataa gcatagaaga tggaatcata aaaagtgatg aggtgatgta
     4261 gatgaattta tatacatcac taaccattag atacttaaaa caaaataaaa gaagaaccat
     4321 tgtaaccata atcggtataa tactttcaac agctttaatt tgtggtatag gaaatatatt
     4381 tgaaagtttt atggattatc aaatgagaga aactataaag aatgatggaa gtttccatgt
     4441 tacattttat gatgtaaata gaaaaaatgt ggaatatgta acgaaatcag ctgaaataga
     4501 aaaacatgca tttaccaaac aacttggata ttctaaatta gaaaatagtg aaaatgctat
     4561 attaagtata aagcaatatg atcaaaatgc aataaacgga tataaagttt ctataaaaga
     4621 aggtagattt ccagctaagg aaggagaaat tgttttaagt gaaagtatta ttaatcttat
     4681 agatgataag ttaaaaatag gagataagat aactttaaaa gtaggggata tgtttgactc
     4741 aagcaagaaa aagattgata gtatgacatt tcatgaaggt gattatatag caaatgaaaa
     4801 agatagaact tttaaagttg ttggtataat taaaaaacct gggtttgaac gttataatgg
     4861 aattgcaaca gctatagcct actttaatcc tatgaataat tacaatgata caattaatgt
     4921 atcagtagca gtaagaaatc ctaaagatgt gtataagata agtaataaaa taagtaaaaa
     4981 tatatattca aataaagata aagaagcagt ttctgatatg ataaaataca atgaacattt
     5041 attgaggtta caaggtgcta gtaagtattc aaatataaat agctctataa agtctataat
     5101 tatagcagta actattttag taatcatatg tacaattgct acagtgtaca attcctttag
     5161 tatttctata aatgaacgaa aaaaacagtt tgggatatta aattctatag gtgctacatc
     5221 tagtcaaatt aaaagattag tttttataga aggtattata attagtttaa ttggtatacc
     5281 aataggacta atatctggaa cagtagctat agacctattg tttaaaatta taaataagta
     5341 ttttactgaa tcggttgtta ctaagatgag tttgcagata gtgtataatc caataataat
     5401 tattgttagt ataataatag ttttgcttac aatctttata tcaattctac tacctgcaat
     5461 atcggcttct aatatttctc cccttaatgt gattaaaaat actggagagt ataaagtggg
     5521 caaaatcaaa agctcaagac ttattaaaat gatttttaaa actgaaggag tacttgcata
     5581 taaaaatatt aggagaaata gaaaaaaatt tataataact cttttttctt taatggtaag
     5641 tataataata tttatatctt ttagtggctt tacattactt ttactaagag gtgaagaaat
     5701 tagaaattct caaagaaaat atgatttgta tttaactgca aagaacacag ctaggtctgt
     5761 agaggatact ataagtgaac tagaagatat tgatgggata aaaaactttg aattggcaac
     5821 aggacaatat gtgtctataa gagtaagtga aaataaaata aataaatcca aggaagattt
     5881 aatcaggaaa tattatcaaa aatataagat tggagaaagt tatgaatatg atttcagtaa
     5941 taatgaattg atttttccgg gagattttgc agtaaaaaac aaaataaata acttagtaca
     6001 gggctctttt aataaagaga gagcaattga agaaaatggt gttatattag ttagaaaaag
     6061 tgcaattgaa gaaaatggga agaaaggtgt agtagaactt accaactata aagtaggaga
     6121 tacagtaaat tgtgaatatc ttgatgaaaa tgccttaagt aaaaaagtaa aggtcaagat
     6181 tcttgctata actgatgaag aacgtctagg ttttggttat caaaatatgg ggttacaatt
     6241 tataacttat gatgaagttg ctaaaaattt aaatttaaaa ttaagtaaaa gccttatatt
     6301 tattgattca ggaggaagca ttaagactaa gaaggaaata gaagctttgg caaataaaaa
     6361 tgattttaac ttttatgatg aaatttcaag cagtgaaagt gaaaaacaag atttaaaggt
     6421 tattaaaata tttgtatatg gttttatagt agtgatatct ttagtgagtg ttacaaatat
     6481 tctaaataca gtaagtacaa gtataaatct aagaaaaaga gaacttgcag taatacaatc
     6541 tataggggtt actcctaaag gatttaggaa aatgatatat ttagaaagtt ttatatatgg
     6601 tatactgtca ttgttgtttg gaataccaat tagtattggt ataattctaa ttatgaataa
     6661 acttatttca ggtgtaattg aattttctcc gattataccg tggacagcca tagttatctg
     6721 tatagtaagt gtttttataa taacttttat agcagggtat ataccaatga gtaaattaaa
     6781 taaagaaaat ataatagata atataagaag agaaagtata tagatataaa aataattctt
     6841 aactatataa aatattatta aaatcattta agtggtataa aatattatta tatcacttaa
     6901 atgatttttt atttatataa gaaatctaat aataaattac tagaggtatt taactattta
     6961 gaaagaattg gtattatata aagtaagaga atatataata agaaagaata tttgatttta
     7021 tttgaaaaca aaagaaaaag aaggtgatta ctatttctca tataactgcg aagaatatgt
     7081 ataaaagttt agaggaaaga ataaacaagt ttccacaagg tgctccacca tctgatacat
     7141 tatacaaaat actaaatgta ctatatacag aacaagaggc gaagttagtt gctcagcttc
     7201 caataaagcc tttcagagta aaaacagcag ctaaaatttg gagtgtaagt gaaagtgaag
     7261 catatagagt attggataaa cttgctagta aggctctgat actagatata gaagataata
     7321 aagggaagaa atatataatg cctcctccaa tggctggttt ttttgagttt gccatgatga
     7381 gaacgagaca cgatatagac caaaaacttt tggcagaact ttactatcaa tatatgaatg
     7441 tagaagaaga ttttatcaaa gatttatttt attcaacaga aacgaagctt ggaagagtat
     7501 acgttcaaga ggaagtttta actaatgata atgaggttag tattttagat tatgaaaggg
     7561 ctactcatat aattgatgaa tcaactcata taggtataag tatgtgttac tgtagacata
     7621 gaatgcaaca tgttggaaaa gcttgtgatg ctcccatgga tatatgtatg acgtttgaca
     7681 atgtagcaaa ttctttaata aataataaat ttgcaagacg tgtggataaa atagaatgta
     7741 aagaactact tcatcaagca tatgaacata acttagttca atgtggagaa aatgttagga
     7801 aaggggttac gtttatatgt aattgctgtg gatgttgctg tgaagcaatg gtagcagcta
     7861 aaagatttgg aaatcttcat ccagtacaga caacaagttt tataccaaat ataaaccatg
     7921 aaaattgtgt aaagtgtggt aaatgtataa ctgcatgtcc aatagatgcg ataagcaagg
     7981 ttaaggaaga tggtaaagaa tatataaaaa ttgatgagga tagatgttta ggttgtggag
     8041 tttgtgttag aaattgccat aaaaatagca ttatgttatt aaagagagat gaaaaaataa
     8101 taactccagc aaattcagta catagagcag ttctcatggc aattgaaaaa gggcagttac
     8161 aaaatttaat ttttgataat aatgctctgg ctagtcatag agctatggga gctatactgt
     8221 cagctatatt aaagcttgaa ccagctaaaa agattttagc cagtaaacaa ttaaagtcag
     8281 tatatttaga taaattgtta agtatgaatg ataaatagaa actaacttaa aaaaggcaaa
     8341 gttttagact tatctatatc ttaataaact tatcatttaa tatttaaatt taatttttac
     8401 acatataaaa cttataactt aaataaaaaa aggctatatt caattaatat tacttgaata
     8461 cagccttttt tagttatgtt aagctatttc ttttatttgc ttaaatgagt tttcatttcc
     8521 atttccacca atttgtgtta cagatttaaa tctcatagtt ttaaatagtt ctttttgatt
     8581 ataatctagt gagtttccta ctagcattac aggagattta gtttttccag ccaaaactcc
     8641 aacagataat ccgtctatta aatcatcttg tttattcatt ccatttttag caacatataa
     8701 attgtctatc ttagaatcct tgtaaaattc ttttattact tttgcattag tttcatttct
     8761 agtgttacct gatattctct gtggattttg aagtttactt tctatatttt tagaaactgt
     8821 atattctcca ccgattacat aagatttatc aatctttttg tttttaaata agttatttat
     8881 ttcagttata ttactatttt cattagtaag tattataggc atatcatttt gagctgatac
     8941 agctccaatg cttactgcat cggctaatcc tttttcgcca ttaactactg aaatctttga
     9001 tatattgcta atattgttca attctttagc aatgttcata gaagtttgat atctatcact
     9061 tcctgaaatt ctagatacag agatattgtt tgattttata gtatctaaag atttttcatt
     9121 tatagaagcc tctccaccaa ctacatatac atttttaact tttaatctct ttaattcagc
     9181 caaagttttt tcatttattt gagagcttcc agtcaataat ataggagcgt ttttcttgta
     9241 tgcaaatggt gttgcggcca aagcatctgc aattgctgaa tcgtttatta aaactgcatt
     9301 ttcagcattt tcccaacctg cttgacttat cttaatagaa gtttcatatc tatcagttcc
     9361 tgtaagtgca cttgcattta cttcatcttg atatgtaacc tcaaatctag catttggact
     9421 tcctaagaaa acaattacgc ctccttttgg caatctaact ctattgttct ttgtaactaa
     9481 agaatagttt acaggaacac ctttatcatc ataaacagca aaagctgaat tttgaggaag
     9541 attaacttct atttttttat ttgcgatatc gtcaccaatc ttataccatt ttgcataacc
     9601 attggattct agttcacaag taaaagattt ttctgctggc aaattagtta tagaatcttc
     9661 actaacatac gttagtgttc caaaagataa atactctgta ccattttctt tatgaagttt
     9721 aatgtcggat aaatcacgac ctattacacc aggtatttct ataaaagcat ttgagttatt
     9781 ctcatccatt attttagtgt taactatata acctggagtt tcatcagaaa gactaaaact
     9841 tgcttttaca gagccaaaca tatagctttg agaagtatat ttttcatcca ctagatagta
     9901 acctttgcca cttctttttt tccacacttc tttgacacta tttgatatat tgttgtcatc
     9961 tactttttga gctacatagt ataaacttgc tgtctgacct aatccaggga catcatcata
    10021 agagctcata tttaaatatg tgatattatt tttttctttt acaaatttta aacaactgtt
    10081 acccttttca gatacaaatc tatcttgacc tatgtatacg tatttatctt caggtccatt
    10141 ttctatataa ggagaagata ctgttaaagt accattatca ttaacatcta cttttatcat
    10201 atttgaagaa gcataaagac cactattttc ttttagagag ctaggcattt taacttgttg
    10261 tggcttacta aatgttttat caggttttat ttctttaatt ttaccttttt cttttaatgc
    10321 agatagtaag atttcttgtc caattatttc gttaagttga ctacttccac cagaagaaag
    10381 aactgcaact gccatatttt catctggaag aactataaga ttactgtgga ataataaaga
    10441 gtctccacct tttgtaagag cttttaaatt atactgatta aatggataag tgttgacaca
    10501 atcccaccct aaaccatatc caagtataga atcttctcct tcaggccata gaccatttaa
    10561 atattctttg ttttccatag cttttactga agcaggagaa agaataccat ttgaattttt
    10621 cataaatgtt tgtgcaaatg tacatagatt ttctgcactt gaatataaac cacctgcacc
    10681 tatagcatta aggttatctt gtggaactgc atcttcccaa taaggtacat atgcttttgc
    10741 tagttttgaa ctatcaaaac tattttctgt agtttttgta ttttgtaagt taagtggatt
    10801 atttatgtat ttatcaagaa aattagtaaa tgacatacca gatactcttt ctactaaaat
    10861 ttcagctaaa gtaaatccat cattacaata aacggaaaaa gctcctggtt tagcttttaa
    10921 tctttgtttt tgtaattctt ttaagaaatt atcatgacca taagaatcat tgtctgctaa
    10981 taaaatagtg tttttaaaac tacttcccat caaccctgat gaatgattta ataacattct
    11041 tggagtgatt tccttgtatc tatcatcagc cattttaaat tcaggtatat actttacaac
    11101 aggagtatct aaatttaatt ttccatcatc aaccaacttc ataacagctg ttgtagtaaa
    11161 cattttactg atagatgcga tactatacat attatcctta tttaagtttt tgttatcctg
    11221 tttgctgtaa actccaccat taccagaaat ctcaattttg ccattatcaa tcaaagcata
    11281 ttgagcactt acagcaccat actttgatag caaagtttgg attttctctt tagcaagagc
    11341 tttagttttt gtgtaagatt ccgcgttttt actataatca tgtaattcta tatcagaact
    11401 ttgatatttt tcggaattat cttttgtatt atcagcaaaa gtaatatttg gagcagcagt
    11461 aaataacata ctcaatacta atgtactaga taataattta gagatccttt gcttaaacat
    11521 attacattac ctctttccct ttttaataat ttataattag cataacattc ttaattattt
    11581 taatcaacag attattatca ataaaaaaag cttttataag tatagctttt tatataacag
    11641 agaaaaatat aaaaataata tttgttgttg tataaagaat aaagggtata ggtaatatat
    11701 aaataaaatg gaggaatttt agacatatgt ataagaatat aaataagaag caacttaaag
    11761 aaatgatgaa aaatgaaaag gatgttttat tattagatgt aagaactaaa gatgaattta
    11821 aggaatataa aatagaaaat gctataaata tatctttaca agatttaatc aataatatag
    11881 atgaaattta cgattataaa gataagaaag tagtagttta ttgcagaagt ggtcatagaa
    11941 gtgttactgc atgtaatttg ttagctgaag aaggttttga gcatttatat aatttaaatt
    12001 caggaataat tgattatatg agttaaataa ggtaagttat acattaaata ctatgtatag
    12061 ctctgtttat taagtaggaa tatatctaaa tatttataag ataaatttag gaaagaacga
    12121 attttttcgc ttttttctat gacagttttt gtagtaaata ttagttttta agttggaaaa
    12181 aggtagtagc tttttagcta ctacctttta atatgaactt agaatattat taaaaaaata
    12241 tatttaaaga cagaattaat tttttaatga ccagttaaat tactatcgcc aaatttatca
    12301 ttaaatggtt ttataactct atttataggt ccttttagaa gtacgtttaa acaaatagac
    12361 agaatcataa atagtaatag aactagtata tctttagtta gatttggagc atatatacca
    12421 ccaactgctt cccttgtagc acctattgca taggtaaatg gcattatagg gtttattctt
    12481 tggaagaatg taggtgtaac ttgtataggg aatgtaccac cagaagaacc aatctgaacg
    12541 actaatagta ctattgcaat tgcttttcct acatttccaa atacagaaac taaagaatac
    12601 actataaagt taaataccat actagtaaac ataagcagaa ctatcaataa aattggatgt
    12661 tttgctgtta ctcctaacaa taatatatct ccagttccta ttataaaacc ttggacaaga
    12721 gctatactta aaaaagtaag acctcttcca aagtaaattt cataaggttt atagtttcca
    12781 tgtacagatg tagacaatag tgctgaaata agtaatatac caacccatgt agaaagaaca
    12841 ctataaaatg gtgtcattgc agaaccataa ttttcaattg gatataattt ctctgttttc
    12901 acctcaacag gttcttttaa gaaattagac tttgtcaata tatcactttc aagcaagctg
    12961 actaattttt tcatatcttc accattacta attttgctta aagtatcaat caaatcatcc
    13021 aacattcctt ttgctaaagg aagtctttct cttatcaatg atatactttc ctgtgcattt
    13081 cctgaaagtt ttagagaagt agtaagtatt tcttcaacct ttggaagttt tgcttctgcc
    13141 ttatctaaaa cagtaattat atcatttgct acttttatac tgtttgcaaa tatactattt
    13201 ataggttttg atatttttga gtcaaaatta tttaaaatat ttaaattaat tcttccaata
    13261 ccatttgata gacttaaaac attattcaaa gcacttatag atggttgttg tccagatata
    13321 actttgtttt taatatcatt taatgttgat atagatgaat ctattttatt gctagagtct
    13381 tcaagattat ctattacatc atccaatcta ttatttgatg taagttggtt taatttagtt
    13441 aaaaagtcca ccaaagtatc gtttaaagat tgcaaattag aaagtttttc tgataaatta
    13501 tctatcaact taggtacatc ttcactgcca ctatttactg catcaattaa atttaatgta
    13561 agagaagaag cagaagatga aaggtcaacc attaaattta agtcagactt aataagagga
    13621 gaaagttcgt ctaaattatc atttgtatcc tctaagaatt tttttaagtc acttgataat
    13681 agttttgtat cattcagagt ctttttaatt gtaggtaaat cagatttaat atctttaact
    13741 gtatcagata attttgaagt agcatcagaa gctaaatcca cagtcttatt tatatggtca
    13801 aatctctttt gaaggtcaat aagggaattt tcaacttttg tcaattttgg aagactgtgt
    13861 tcaattcctg caccaactcc gtttgatata ccaagaacga tttcactaac tgtttttact
    13921 atagtctgat taacctgtag ttgtattgta gaagcacctt tatctgtaat ctttggtgca
    13981 actgcattta ttttttcatt aacagtatag ataatttcgc cttttttaac atcatctgta
    14041 attaaagatg tcaaatcttt tgtaaaattt tttggaatct taagtgatgc ataataagta
    14101 ccttttttaa caccttcaag agcagttttt tcatctacaa actgccaacc taaactcttg
    14161 ttatctttga gttgttcaat taattggtct cctacattaa tcttttgtcc tgctatttca
    14221 tttcctttat cttcatttgt tactgctaca agcatattag cagtgcttcc ataagggtcc
    14281 caagatgcct ttatattgaa ccaagcatac agagatggta aaaaagcaag acctaaaact
    14341 attacaagta cggcataatt gccacgtata tcttttaggt cacttttaaa aatttctaat
    14401 atatttttta aaccctttga aggtcttaaa ttcttgatat tattccagtt taaaatttct
    14461 tttaaactat tagttttttt caaatgtctc acctcctaaa atattttgat aaagaaaaaa
    14521 gttagattta taatcaattt taaattatat tactttttac acaaatagta aagtta
//
LOCUS       DACTGW010000084        14558 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.084, whole genome shotgun sequence.
ACCESSION   DACTGW010000084 DACTGW010000000
VERSION     DACTGW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14558
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.084"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            761..2431
                     /locus_tag="I9573_001971"
     CDS             761..2431
                     /locus_tag="I9573_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT4796591.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2553..3287)
                     /gene="pflA"
                     /locus_tag="I9573_001972"
     CDS             complement(2553..3287)
                     /gene="pflA"
                     /locus_tag="I9573_001972"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAT4796592.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3447..5678)
                     /gene="pflB"
                     /locus_tag="I9573_001973"
     CDS             complement(3447..5678)
                     /gene="pflB"
                     /locus_tag="I9573_001973"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAT4796593.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFS
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5971..7032
                     /locus_tag="I9573_001974"
     CDS             5971..7032
                     /locus_tag="I9573_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAT4796594.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7123..8736
                     /locus_tag="I9573_001975"
     CDS             7123..8736
                     /locus_tag="I9573_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAT4796595.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9011..10996
                     /locus_tag="I9573_001976"
     CDS             9011..10996
                     /locus_tag="I9573_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT4796596.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLSFGILEKPVVWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMIEELAKVKSFDEFYKIITKRR"
     gene            11001..11435
                     /locus_tag="I9573_001977"
     CDS             11001..11435
                     /locus_tag="I9573_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT4796597.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11464..12024
                     /gene="srlA"
                     /locus_tag="I9573_001978"
     CDS             11464..12024
                     /gene="srlA"
                     /locus_tag="I9573_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012423036.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAT4796598.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12088..12456
                     /locus_tag="I9573_001979"
     CDS             12088..12456
                     /locus_tag="I9573_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HAT4796599.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12481..13047
                     /locus_tag="I9573_001980"
     CDS             12481..13047
                     /locus_tag="I9573_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAT4796600.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13088..13453
                     /locus_tag="I9573_001981"
     CDS             13088..13453
                     /locus_tag="I9573_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAT4796601.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTNDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13554..14327
                     /gene="srlD"
                     /locus_tag="I9573_001982"
     CDS             13554..14327
                     /gene="srlD"
                     /locus_tag="I9573_001982"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAT4796602.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIETEFGCKSVGYGCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
BASE COUNT         5582 a         1720 c         2449 g         4807 t
ORIGIN      
        1 gtatttttta ttaattctta taaaatcaat gaaaataagg atatccattt taaagtacat
       61 aaatttaaaa atccagaaaa atatacttaa attatgtaag caaaagaggg gttgaatcgg
      121 ctgaaaatgt aatactagta aatagtacat caatcctaga taccctgtaa gcaactctat
      181 tttcatgtac taaaaatgta cctattttat taactgaaaa gaataaatta aatgtaagta
      241 cataataaga gataaaaaat taaaagcaaa aaatatttat ataaataaat ataaaaaatg
      301 atataatccc tattaaaaaa tttatagatg atgaagaaat atcaaaatct tatattatag
      361 gaagaggcac ttctttgcca aaatatatag aaaagcaata ccaaatccaa agagaatagg
      421 ctgagaaaaa ataaaacaaa ttcaagagta atagaaaagc tttatagaat aaagaactga
      481 gaactcttta tataataaaa aatggacttt taagagaaaa tgatttaata aatgatttaa
      541 catggtgttt tagcaggtga aaataaatca ccagtaatga ttgtagaaaa attactttat
      601 gaatctcaaa aaaacttagt taagagtaag agatttgata agataactaa agttggggaa
      661 atgtgtttga tgagataaaa aaattagtaa aatagtataa tctaaataag aatattttgt
      721 tgattaaact acactaatag taatattgta gggggaaaag atgagagaag aatcgaactc
      781 tttaagttta gaccatatag aaattttctt tgaactttta tctcaaagta cagaagatta
      841 tatatttttt tgggatataa ataggaataa atttaaaatt tcttcagcaa tttttgatga
      901 atttaactta agtaaagaga tagaatctga tttagtcaat tgttggagta aaattgttta
      961 tccggatgat gttcaaatat ggaaagatga tatacaagaa ttactacatg gaaaaaaagg
     1021 tgagcacaat ttagaatata ggttgattaa taaatatgaa gaaatagtat ggatttcttg
     1081 tagaggtaaa gtttatgtgt cggatgaccc aaaaactata tttctagtcg ggagaataaa
     1141 gaatattggt gaaaaaaata aatttgattc tatcacagga acgtggaata gagaacaatt
     1201 tgaacataga atgaattatc ttataaaaga gaaaatttat aaaaatggtg ctatgtttat
     1261 catggatatt gataatttta aaaatataaa tgaaaaatat ggacattctt atggtgacaa
     1321 agtattgcgt gcaattgcta ctgaagtatt agaatatctt ccaaaagatg taagattgta
     1381 taggttagat ggagatgaat ttgcattttt ttaccctatg tgtactaaag aaactataga
     1441 gaaaatatat gaaaagatac agatgtatac aaatactcag catgaaattg aatcaaataa
     1501 gtactattgc acagtgactg caggagttgc tatgtatcca gaagatggag ataattattt
     1561 agatttattt aaacatgctg atattgcatt ggatatagca aaaattagtg gtaaaaatag
     1621 aattaagttt ttttcacaag agctctatga aaataaacta aaggtaattt caatgcaaca
     1681 aaaactaaga gagtgcgttg aaaataattt taatgacttt gagctgtttt ttcaacccca
     1741 ggtaaatgct gttacaaaag aggtaatagg ggcagaggta cttttaagat ggcattcgag
     1801 tacttatgga gaagtatctc ctgttgaatt tataccaata ctagaacaga gtaatttaat
     1861 tataccagtt gggaagtgga ttataaaaga agctgtaaaa cagtgtaaag aatggcataa
     1921 aataaatcca gactttaaaa tatctgtaaa tgtatcgtat attcagttaa aagaagattt
     1981 ttttagagat tttatagtag aatgtttagt agagtatcaa ttaagacctg aatttttaat
     2041 tttggaatta acagagaatt gttggatacc agatataaat ttactaaatg ataaatttat
     2101 tagtttaaag ggtataggag tatatattgc aatagatgac tttggtacag ggtattcatc
     2161 tttaaattac cttaaagaac tgtctgttaa tataataaaa attgaaagaa gttttgttaa
     2221 gaatattaca tataatagtt atgaatatac ttttcttgaa tatattatta aattagctca
     2281 tattataaat ttaaaagttt gtgttgaggg aatagagtct tatgaagagt atgatatagt
     2341 aaaaagctta ggtgtagata ttattcaggg ttttttattt ggaagacctg taagtgcctc
     2401 tgaattttat aaatttcaac tatcaaagtg atgtacataa aatgagatat atagtatata
     2461 aaaatagggc aaagtggaag ttttgaagtt tattaaaaat ttacacttta ccctattttg
     2521 ttatgtcttc tataaaggat taatatacta cactaactat tatatacttt cactccaaat
     2581 ttagagatta tttcactagc tttttggaca tcttccttag tggcatcagg aacatctttt
     2641 agttcataat cgatacctaa actttcccat ttgtgtattc ccatagaatg gtatggtagc
     2701 aattcaaatc tttctacatt tttcaatgta gctacaaatt gacctagttt ttctaaatta
     2761 tctatatcat cagtaatgcc aggaactaaa acatgtctta tccatacagg aatatttctt
     2821 gattctaagt gtctagcaag ttttaatgcc ttatccatac caacaccagt caaatcaata
     2881 gatttttcct caaccatatg ttttaaatca agtaaaacca aatcagtatt atctaaaact
     2941 gggtctatag tttctatatc tacgaatcca gatgtatcca agcaagtgtg tatatcattt
     3001 tcttttgctt ttttaaataa ttcacttaaa aattctggtt gaagagtgga ttcaccacca
     3061 gaggcagtta tacctccacc agaaaacttc ataaaagaag tatacttaag agcatccgtt
     3121 attatttcat caactgtgta ttcttttcct gatttaatat cccatgtatc cctgttatga
     3181 cagtatttgc atctaagagg acacccctga aaaaataata tatatcttat tcctggtcca
     3241 tctacagtac caaacgtttc tatagaatgt atctttccct taatcataga gttcacctca
     3301 caatatattt taataacctt atcaatagtt ataatatttg tctaaaaaag gccatcttat
     3361 accagaaata attcaaatat aagatagcct tttattttta ttttttacac agtagtaatc
     3421 ttagttaaag tataaaaatt taaagactaa gccattttac cgtggaatgt tctgtttata
     3481 acatctaatt gttgctcttt agttaatttt atgaagttaa ctgcatatcc agatactctt
     3541 atagttaatt gtggatattc ttctggatgt tccatagcat cttctaaagt agctctatca
     3601 aatacattta catttaagtg atgagcattt tgagagaagt atccatccat cattgcagaa
     3661 agattgttta ttctttcagt catatctttt cctaaagcag caggtactat agagaaagta
     3721 ttagatatac catcttgagc atgttcatat ggtaatttag caactgaaga taatgaagct
     3781 aaagcaccat tagtatctct accatgcatt gggttagcac caggagcaaa tggagcacca
     3841 gctcttctac catctggtgt attaccagtt ttcttaccat aaacaacatt tgaagttatt
     3901 gtaagtatag attgagttgg ataagaattt ctataagttt tattttttct tattttattc
     3961 ataaagcttt caactaactc tacagctata ctatcaactc tatcatcatt gtttccgtat
     4021 tttggataat ctccttctat ttcaaaatca acagctattc cttgttcatt tctaatagtt
     4081 ttaaccttag catgttttat agcagataat gagtctgcac aaactgataa cccagccata
     4141 ccacaagcca tagttctaaa tatatctcta tcatgtaatg ccatttctaa tgcttcataa
     4201 gaatatttat catgcatata atgtattaca tttaaagtat ttacatataa tgttgctaac
     4261 caatcagtga atggttcaaa tttactcata acttcatcat aatctaagta ctcagaagtt
     4321 ataggttcaa atcttggtcc aacttgaaca ccagatttct catctacacc accatttata
     4381 gcgtataata aagttttagc taagttaact ctagctccaa agaactgcat ttgtttacca
     4441 attctcattg cagatacaca acaagctata ccatagtcat ctccccagta tgctctcatt
     4501 aaatcatcat tttcatactg tactgaactt gtatctatag atactttaga acagaaatct
     4561 ttgaatcctt gaggtaattt agttgaccat agaactgtta agtttggctc tggagaaggt
     4621 cctaatgtat atagagtatt taacattcta aatgagtttt tagtaactaa tgttctacca
     4681 tctattccca tacctcctat acactcagtt acccaagttg ggtctccaga gaataggtta
     4741 ttgtaatcag gagttcttag gaacttaacc attcttaatt tcataacaaa atggtccata
     4801 tactcttgta attcttcttc tgttacgatt ccagctttta aatctctttc aaagtatata
     4861 tctaagaaag tagatgttct acctaaagac attgcagcac cattttggtc tttaacagca
     4921 cctaaataac caaagtataa ccattgtaca gcttcttttg agttagttgc tggttttgat
     4981 atatcaaatc cataagattc agccattttt ttaagttcat ttaaagctga tatttgctca
     5041 gttatttctt ctcttaatct gataacttcc tcatccatgc aagaaacttc taatgatttc
     5101 ttttgctcat ttttatcttc tattaaagca tcaacaccgt atagagcaac tcttctgtag
     5161 tcaccaatta ttctacctct accataagca tctggaagac ctgttattat accagatttt
     5221 ctagcagctc tcatttctgg agtataaaca tcaaaaactc cttggttgtg tgtttttctg
     5281 tatttagtaa atatatcttt tatttctggg tctaattcat atccaaaagc ttcgcatgaa
     5341 ttttcaacca tctttatacc accatatggc atgacagctc tttttaaagg ggcatccgtt
     5401 tgtaacccaa ctattgtttc taattcttta tctaaatatc ctggagcata tgcagctatt
     5461 ccagaaacag tttttgtatc aacatctaaa gtaccaccat tttctctttc ttttttaaat
     5521 aggaccattg cttcgtccca taatttttta gtattttcag tggcaccagc taagaaagag
     5581 tcatttccct catatggaga atagtttaat tgtataaact ctctaacatt tatttcttta
     5641 gtccatcttc ctgttttaaa tccttgccat gcattcatta agtatttcct ccttagattt
     5701 aaatgataaa taatactatt aaaattattt tttatatact taatttattt tatatatttt
     5761 tttaaaaata tattttctaa tataataata tcatattatt cgtatcctgt atacataaaa
     5821 aataaaaaat tgtcgcttta aagtataaaa cagattatat gttaataatg tggtgaaatt
     5881 atacccatat ataaataaaa taaaacaaat aaatagctaa ttaaggagaa aatatttttt
     5941 attttttgtt taggataaat aaattttatt atggaaaaaa gaaaaagcat acatcttcac
     6001 aaattgaata tttatttaat agggggaggt gatagattgt ttatcacagt ggtattattt
     6061 ttagtaggtt tcctattaat tactaaaggc gcagatatct ttataaattg cactgtagag
     6121 ataggtagaa aaacgaatat atcagaaatt atacttggag ctactatagt tagttttgca
     6181 acaacattac ctgaatttac agtgtctcta cttgcttcta ttgatggtca tactactatg
     6241 agtttaggaa atgcagttgg ttctataata tgtaatacag gattagcatt agggttggtt
     6301 gtttttataa gtccatttaa tgtagataaa aaaatgtttt tttctaagtc tttactatta
     6361 atagtatccg ttatagtttt aattttattg agtttagatg gagttataac aagaggtgat
     6421 tcattattac ttataataat attaatcttt tatatggtta ataattatag aagtgtcgtt
     6481 ggaaaatctg atactaaaaa tagaaatatt aaaaataaca ctattaaaga catatctact
     6541 aaaaaaggga aaaattatag aggtttttcc gtattagaaa ttgtaaaaat actattactt
     6601 tttgctacag gattgataat gatgataata ggttcacaaa tccttataga gagtggagta
     6661 ataatagcta gttttttaaa tatacctcag ggtatagtaa gtttaactat aatagcactt
     6721 ggaacttctc tgccagagat agtttcgtcg attactgcta taagaaaaaa tcatcatgaa
     6781 atatcagtag gaaatatttt gggtgcaaac atattgaata tagtatcagt aatagctgta
     6841 tcagcaatac caaataacat accaatacta tctcaaaata gacaattgga tataccattc
     6901 atgatattat tattattaat agtaataata ccaacactta aatctaatag acttagtaga
     6961 atacaaggta tattgatgtt attcacatat tttctgtaca tatcaatttt gtatttcatg
     7021 tatattattt aactatactg tttaaaatat taattgcaga atatcctaaa aaagtatata
     7081 atggataggt taagattaat tgataacgga gaggaataat atatgaatat aacaaagttt
     7141 gaagatttac caataagtga gggaataaaa aaagctatag ctgaaatggg gtttgaagaa
     7201 ccatctccta tacaagcaca gtctatacct gcgattttgt caggtaaaga cgttatagga
     7261 caagctcaaa ctggtacggg aaaaacagct gcttttagta tccctatact agaaacaata
     7321 gacccaaata atagaagttt acaagctgta gtgctttgtc caacaagaga actggcaata
     7381 caagtatcaa cagaaataag aaagttagct aagtattcgc acggaataaa gacattacct
     7441 atatatggtg gccagccaat agatagacaa ataaaatctc taaagagtgg tgtgcaagtt
     7501 gtaataggta caccaggacg tacaatagac catatcaatc gtaaaacatt aaagatggat
     7561 aatgtaaaaa tgattatatt agatgaagca gatgaaatgc tagatatggg atttagagaa
     7621 gatatagaaa tgattttaag taagattcca gaagaaagac aaacaacttt cttttcagct
     7681 actatgccaa gaggaatttt ggaattaact aagagatatc aaaaagaccc agagcatata
     7741 aaagttgtta gaaaagaact tacagtttca aatacaaagc aatactatat agaaactagg
     7801 tcatcaaata aattagaagt attatgtaga ttagtagatg tatatgaccc taaactatct
     7861 gttgttttct gtaatactaa gagaaaagca gatgaattgg taggagattt acaagcaaga
     7921 ggatattttg cagatgcatt acatggtgac ttaaaacaga ctcaaagaga tatagtaatg
     7981 gataagttta gaaatggtac aatagatata cttgttgcta ctgatgttgc agctagaggt
     8041 atagatgttg atgatgttga atgtgtattc aactatgact taccacaaga tgaagagtat
     8101 tacgttcata gaattggtag aactggtcgt gctggtagag aaggtatgtc atttactttt
     8161 gtatttggaa aagaaatgag aaagatgaaa gatatagaaa gatatactaa atctaaacta
     8221 ataaagcata atataccaac aataactgat gttgaagaga aaaaagtagg tactttcttt
     8281 gcacaagtta aacaaactat agaagaaggt cacttaacta aacaattaca atggttagaa
     8341 ggtttctgta atgatgaaga ttatgcaatg gttgatatag ctgctgcact tgtaaaacta
     8401 tctctaggtg aagaaatgaa agaagaaata atagaagaaa aacctagaag agagcgtgga
     8461 gatagaaaag gtggtactgg agctaaagat ggtatgatta gattatttat caatattggt
     8521 agaaatcaaa gagtacaagc aaaagatata gttggtgcta tagctggaga agttggaata
     8581 ccaggtaaag tagtaggtac tatagatata tatgataagt atacttttgt tgaaatacct
     8641 aaaaaagatg caaaaacagt aattgaaaag atgaaagata ttaagataaa aggtaataag
     8701 attaatatag aaaaagcaaa taagaagaaa aaataacgta tagtatacaa aactataatt
     8761 agaaaatata taaaataata tattggatat ttaaaaagcc tgatgtgttt tcaaaacaga
     8821 tagtagataa tctgtaagag ttaacagcat aaggcttttc ttgttttata tgatattatg
     8881 attttccaga tcgttgtgaa aaatgtatat tagttccttt ttttcaaatc tgatttgaaa
     8941 aagagaatat taattatcta tatagtttag attataataa aaaatgtaag ataacaaagg
     9001 ggagaaaaag atgtcaatga cttatatgga ccctagggtt tttttgattt tagaaatggt
     9061 gttagaagaa gacacaaata ctctagaaca attttcaaat gttttgggtg ttagtactag
     9121 gactatcaga aactatataa accagattaa tcgagaatta aaaggtggta ttgctaaaat
     9181 agttaaaaat gaaaaaggta catatagtat aagaattgag gatgaaagta aattaattga
     9241 gattgtaaat tttaatagag gaaagctatc aaattttata aatttaaatt cacctgatga
     9301 aagaataaat tatgttcttg atattttaac tatgaccaac aatccaatta ccatagatga
     9361 tttggctgaa gagatatgta taggaagaac aacattaata aaggatttaa aaaaagcaga
     9421 cagaatttta gctgaatatg atttagaatt aaaaaggaag ccaaatatag gtatgatttt
     9481 agttggtgaa gaaatggata taagattatt aatccttgat agattgtatg aaaattatat
     9541 tgatgtcttg gaaaacgtta atcagataaa tttagttaaa gattatgata ttgaatgtct
     9601 tagagatgaa ttaaaaaagt tgtttaaaaa agaagaactc tacataacag aacaggtctt
     9661 gagagatgtt gaaagatata taatcatgtc tgtcttaaga aatctaaatg ggtacaaatt
     9721 tgaaaagata gataagagat ttgaagtgat aagatgttca gatgaatata ctttaggatt
     9781 aaagctcaaa gctttattgg aaaagatatt taatatagtt ttaaatgaaa aagaaaccat
     9841 atttttgaca atgccattaa taggtagaaa agctcctagt acaaaattag cacttagcag
     9901 tataaagata aatgatagcg taaaggaagt tgtagatgaa gtaactagtt ttttattaga
     9961 gacctcaggg attgatttta aagaagataa aaagcttata gaaaatttgg aatatcactt
    10021 gtattttgca cttaatagaa tgagattcaa cataagggta aaaaatcctc ttttacaaga
    10081 aataaaggaa agatatacat ttccatatag tgttgcaaag atagctgcaa tgataattga
    10141 ggaaaagttt gacttgaaaa tatgtgacca cgaaatagga tatatagcat tacattttgg
    10201 aagttatttt gaaaaaaatt caagaaaagt tctaaatgta aatagggtag ctgttgtttg
    10261 tggaacaggt cttgggactg cacaacttat tagaataaag ataggaaaaa tacttggaga
    10321 aagtgtagaa gtaaatactt tttctgatat agaagctaaa gctaatttag atttattaaa
    10381 gtattatgat ttagtattta caacagtaga tttcaaaaca gacttagatg ttccaatttt
    10441 tagagtaaat gcgttattta atgaagaaag tataaagaaa gagatagaaa atgcaatact
    10501 tttgaaaaat agtataaatc ttaagtctat aaaaaagatt gaaaaaaata tcccttttat
    10561 aggtaaatta attgaagagg ataagttctt tatattgaat aagagtacgt ttatggaaaa
    10621 tttagaagat atgttagaaa aattagttca taaaggaatt attgatatag agttcaaaaa
    10681 aaaggtatta aaaagagaaa agaaatcttc aacagcatta gataattata tagcattgcc
    10741 tcactcagtt aatactaatg gggaaaatct ttatctgtca tttggaatat tagaaaaacc
    10801 agtggtttgg gatacaaagg aaattagaat aataatattg atgataattc cagataagaa
    10861 tgtagatagt acagacctga taataaaaag ttatgaagaa gtacttgaag tagggagaga
    10921 taaaaaaatg atagaagaac ttgctaaagt aaaatcattt gatgaatttt ataaaataat
    10981 taccaagagg aggtaataat atggacccag taatttttat tatagcatca cttattacag
    11041 tgtatttatt aaatattttt cttggatact tacagcttaa agattttaat aaaaattata
    11101 ttgaactaaa aagaaaagga agagtggcaa taggcagaaa aaaaggaaga atttcatcag
    11161 gtacaattgt gttgatactt ataaatgatg atggtgtgat tgtcgaaact agaaaaatgc
    11221 aaggtgttac agtattggca agagtaaagt catttgaagg tttggtagga aagagcttag
    11281 agtacataga aaaatcagat ttaactgttt acaataaact acttaaaaaa gcaatactag
    11341 atgcagtcaa acagtacata acatttgaga aaaataaatg tgaagataca aataatctga
    11401 gtactgatga agagtgtagg atggaagtag tttaaaaaaa tatatttata aggaggagtt
    11461 aatatgacgc aattttttca agtattagca agtggtgcag atagttttat gaatttattt
    11521 agggcaggtg ggcaacaatt tgtaggtttt gtaacagata tagtaccact tttagtagca
    11581 ttattagtta caatgaatgc aattatcaac tttattggta cagatagagt cgagagactt
    11641 gcaaaaaaat gttctgcaaa tatatttact agatatttga tattaccagt ccttggtaca
    11701 tttgtatttg caaacccaat gacactatca ctaggaagat tcttgccaga aaagtttaaa
    11761 cctagttact ttgcagcagc aagcttttca tgccatacaa tgaatggatt attcccacat
    11821 atcaatccag gtgaactatt tatatatcta ggaattgcaa atggtataac aactttggga
    11881 ttctcaacag cagatttagc agttagatat ttgttagtgg gtatagttgc aaactttatt
    11941 aaaggtgtag ttactgacta tacaacaaaa tttgtagaga aacaacaagg aatagcatta
    12001 aattcagata taaagattgg ataattactt taaggtataa ataaattttt caaataaaaa
    12061 gatagtgtat agaggaggat aaattatatg gcatttaaca aagttaaaat agtaaaaggt
    12121 tctggaggat ggggtggacc attgataata gaaccaacag aaaagaagaa taaggtagta
    12181 tatgtgactg ggggagcaaa gccagaaact gcagtaaaaa tagctgaact tacaggatgt
    12241 gagttaatag atggattttc acagggtgtt aaagatgatg aaatcgcatg tgtaataata
    12301 aattgtggtg gaacattgag atgtggtata tatcctcaaa agaaaatacc gactataaat
    12361 ataatgaaaa caggaaaaag tggaccatta gcaatgttta taaaagaaga tatatatgtt
    12421 tcaggggtaa aaccaacaaa tgtagagcag ttataattga ggtagtagga gggaagaaat
    12481 atggcttatg atactaaaaa gaagataagt gagcagagtc aaggaattgt tgcaaaaata
    12541 ggtatagcac taggaaatgt gacaagtact ctttttcaag ctggtaggga tactatagat
    12601 acattaatac atactatact gccttttatg gctttcgttg cagtcctaat aggtataata
    12661 aatcaatcag gtataggaaa ttgggtagcc aatttactta ctccattaat cggaaatgtt
    12721 ggaggtttag ttgtcttatc aataatatgt tcatttccgt tgttgtctcc gtttcttgga
    12781 ccaggagctg ttatagctca gatagtgggg gtattggttg gtacagaaat aggaaaagga
    12841 aatattccac caagtttagc attaccagca ttgtttgcaa taaactcaca agcagcttgt
    12901 gactttatac cagtaggtct tggtcttgca gaagctgaac ctgatacagt tcaagtagga
    12961 gttccgtcag ttttatattc tagattttta acaggtgcac caactgttct attagcatgg
    13021 ttagcaagct ttgggttata taaatagaag aatactttat aaataattag agagaggttc
    13081 agttgatatg gaattaatat acagtactca aataaataaa ataggagata atgcaaatga
    13141 atttttacaa cataatatgt ttgtaacttt caaagataat gctcctcaag agttagaaaa
    13201 ttactgttat atacattctg aaaataattt agtaaaagaa atacttacga atgatgtgtt
    13261 atcaataaat aatgttgatt acaaaattac ttctgttggt gagttagtaa accaaaatct
    13321 ttcagaatta ggtcatataa catttaagtt tacaggtgaa aaagatggag caattggagg
    13381 gaccttatat cttgaagaaa aagaaatagc acctattggc gtaggtacta tactaaaaat
    13441 aattagaaaa taaaatttaa aacgaatcta atcatattat aagtaaatta tagataaaag
    13501 tcaatttaac taatcaagtt aacttttaat gtgataaaaa aggggaaatg aaaatgagaa
    13561 aagtggcttt agttgttgga ggaggaagaa gtttaggtga atatttatct aaacatttag
    13621 ctaaagtagg ttatgatgtt gctattgcag atttaaatga agacaacgca aaaaaagtag
    13681 ctgaagaaat agaaactgaa tttggttgta aatctgtagg atatggttgt aatgccatag
    13741 atgaaagaca ggttataagc actgttaaag ctattgaaaa tgattttgat agaatagatt
    13801 tattagtgta caatgcagga gtggctgtaa gtaaaaaaat aactgatttt ggatacaaag
    13861 attttaaatt tattgtagac gtaaatttaa atgggtactt tctatttgca agagaagtat
    13921 ctaaagtcat gatagcaaaa ggtattgagg gtagaatcat acagataaat tcaaaatcag
    13981 gtagagtggg aagcaagcat aattcaggat attcagcagg taaatttgct ggagtaggac
    14041 ttactcaaag tttagcgcta gatttggcag aacataagat aactgtaaat gcactgatgc
    14101 tcggtaattt acttgattct gatatgtttg aaagtttaat tcctcaatat gcagtaaaat
    14161 tgggaatacc tgaatcagaa gtcaagcaag tttatataga taaagtacct ttaaaaagag
    14221 ggtgtagatt tatagatgtt gcaaatatgc ttacatttta tgcttcaaag gaagctgaat
    14281 actgtactgg tcaatctata aatattactg gtggacaagt tatgtaaggt ttttatggag
    14341 tgtctcaaga tgaactcttt agttcatgag gcactttttt tgtataaaat taaatatatt
    14401 ttcatcattt aaacaatgaa aaagaggttt atctttattt tgagatagcc tcatttttgt
    14461 aattaagaaa catagtgttt aagtaaacat ataagattgt tagtttttta aaagagaatt
    14521 ttttaataat attatattta tgaagtattt ttaaactt
//
LOCUS       DACTGW010000085        14550 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.085, whole genome shotgun sequence.
ACCESSION   DACTGW010000085 DACTGW010000000
VERSION     DACTGW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14550
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.085"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(67..603)
                     /locus_tag="I9573_001983"
     CDS             complement(67..603)
                     /locus_tag="I9573_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAT4796603.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENKDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(621..1934)
                     /locus_tag="I9573_001984"
     CDS             complement(621..1934)
                     /locus_tag="I9573_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAT4796604.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEENIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2270..2710
                     /locus_tag="I9573_001985"
     CDS             2270..2710
                     /locus_tag="I9573_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HAT4796605.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLETQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVSLTPKA
                     KELWVTRYTCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2895..3083)
                     /locus_tag="I9573_001986"
     CDS             complement(2895..3083)
                     /locus_tag="I9573_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796606.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            4144..4539
                     /locus_tag="I9573_001987"
     CDS             4144..4539
                     /locus_tag="I9573_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796607.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNNIKCEEIKDNVIEEKNNTCTTE"
     gene            4582..6681
                     /locus_tag="I9573_001988"
     CDS             4582..6681
                     /locus_tag="I9573_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAT4796608.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEISVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLKKDATEILVPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTGIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNHSIAVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKKDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLREYGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVSGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYY"
     gene            complement(6906..12116)
                     /locus_tag="I9573_001989"
     CDS             complement(6906..12116)
                     /locus_tag="I9573_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796609.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRNFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDRQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKTQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLCIPEGIYSINVSGK
                     DAEKLVGEGSIYDGMNGRNGNNAGTSGDILLSFKEIEKRGELKLYVDGGRGGNGQDGK
                     RGDTGITGEAGENAKIGSKKESKEFAKVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKASLLKGAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSAPIDDSDRNYYYFDRYVFNHASNENQVADDGEKLNALYEKHKDWTISKREDSGLV
                     GAEGKDGRSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFIGEKKNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKDELENK
                     MLSVNAKMNLLIQEIIKMKEEEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYAEKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSGSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYIDI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSESIDILKSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIC"
     gene            complement(12131..14254)
                     /locus_tag="I9573_001990"
     CDS             complement(12131..14254)
                     /locus_tag="I9573_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT4796610.1"
                     /translation="MQNSVKQEPCDFSHGRFRPEADILDSRLTKVLDLFQQLNMCLHK
                     DNFRGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFEELLKDNGK
                     NIHLCKPLESDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIKEIQNNIVS
                     SLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTTLTIERFINVLAKQ
                     SGLFLQDSCMELQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEKASKKIMEQ
                     NFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSILTNVTVTQ
                     NEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIRALIPNLVI
                     SSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNLDVGSNVKI
                     NVSGKSGELVDSSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNGSKGIDGKN
                     GETGDSGSNGADAGYTSSFHGQMFKGYFAKGTSGKKGDKGGAGGNAGRGGNAGNKGNI
                     YFISDSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLSFSPSGIDK
                     TVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDDGGFNAPNT
                     KLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(14319..>14550)
                     /locus_tag="I9573_001991"
     CDS             complement(14319..>14550)
                     /locus_tag="I9573_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT4796611.1"
                     /translation="QLKYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLSIREW
                     ICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         4775 a         2660 c         1625 g         5490 t
ORIGIN      
        1 ctttattttc aatatatatt ttaaattttc tctttttaat atatcttttt cttttaataa
       61 atctctttaa ttttcttctt ctatatcaga gccaaaggga atccatgcat taccaaactc
      121 tttatcttta aataaataag ctaatccagt tatttgcttc aatctaagaa tttcatcaac
      181 ttccataccc aggtgctttg atatccattc atcagatttt ccaatttcac aaagttcagt
      241 tataatttta ctcattaaat ccacattatg agaacctctt gcacgattgt gtcgaatagt
      301 acttgccatt ctttcatcaa gagatttatc aattactgaa acaggaagaa gtccgttttc
      361 tctatcatat atatccttat tttcaagcat tactctatat ctatgaaaac catctacaat
      421 aatatactta tccttttcct cattataata acagacaatt ggcattgtat acccatcttc
      481 ttttatactt ttatacaaca actgcatttc tggtggggct actgcatttg gattatagtc
      541 gttagattct atcttttcga ttggcactgc gatgattcca tatacaggac ttttatattc
      601 catagcatct taactcctat ctaatgaatt ttttcatatt tcttcttaat atattccgtt
      661 ctaattctct gttcttttgt aggaccaaat cccataaatc tacacatatg gtcatttttt
      721 aaaatacaat aacacattct tttccaactt ggtatatctt tggtaaattt aatatcatcc
      781 gtatcatcag gtatatcatc caaaaatatg actctactct ttttatcttt tgtatgattt
      841 gaaactccat ttaattctat tttataacct tttctcaaca attcacgtat aactatttct
      901 gacaaaccac cacctgtatt ctgccaaaac tcaatagatg ttgaaaattt tttaatatag
      961 ctattcctta tattttcagg taaggtatca agcaaaaaat atgtgaaact tttccatgtg
     1021 tgtccatatg gcaatttaac actacgatat cccatagctt ttgtttttcc ataaatagaa
     1081 gtaaaattaa ctccttgtac tctagcaacc aatttcaccc atacatctgg gtccaatatt
     1141 ctgtataaat ttaaactctc ttttgctgaa tcattaaaag gtgatgccac tctcattttt
     1201 cttggagcta ctccagcttt atagaataag tcataaattt tattatagtc ataatcaaat
     1261 ttatagtggg ctaaccatat atcttctatt tcccaatcat atataggaga tgccgtccag
     1321 acatttttaa attccttagt tatccagcac tttcctttat atccatgctg tttattcaca
     1381 atagaacaat aacgttgtag agattctgct gctcgtgttc ctaataagca aacaactttt
     1441 gcaccttcat gtatatctct aatccatcta ccaaattgct ttgccaaact ttcttgtctc
     1501 attttatact tataataagt aaaaggatta tttttttgat ttattatgta ttctttgact
     1561 ggcatttctc ttacccataa ttcttctttc tcatcatccc atggatacca atacatttca
     1621 taatttccta cagcagttct tgtagccatt ggtaaacaca cccaatatgg ttcaatatct
     1681 tcaatattct cttcaaatac tttttcaaca tattcagtag tcaaactata ttgagcctca
     1741 aaatcttgat gaaatactcc tattttggaa cttatattat tctttctctt ataatccaaa
     1801 actaaattta gcagtaagcc actatctttt cctccactaa aagatacata gatataatca
     1861 aattcattaa aaagataaga aagtctttct tgaaaagctt caaatacatc ggattctaaa
     1921 tatctcttat ccatttgaat tatccccttt caattagaag tttataaaac cttacaatac
     1981 aatattatta tgctttttta gcagtaatca gcataaatta tacattattt aaatacacaa
     2041 atccaactaa acacatataa ttataatgta attatacttt tgtattataa actatatttt
     2101 atcattattt tacatttttt tccaatatat ataaatatca tatttttcta ttagctaaaa
     2161 ataattactt tatattttta catgttagta taataagaca taaaaacttg acatactgtt
     2221 gtcataatta ctttgctata attaaaagta aaaatggagg taaattataa tgtatgaacg
     2281 tatgcttgat aaaagcaata aaccctgttt gtctcaaata atagaatata taggtaaaga
     2341 tggatatgca cgattgtgta agttggaaac ttacttggaa actcaatata atttatcaag
     2401 agaacttcga tttccttttg gaaacaatta tggttggggt tacaaatata gccataaatc
     2461 ttttcattta ttttatgctt tttttgaaaa aggtgcattt actgttctga ttcagatagg
     2521 tgacaagcaa gttccagcag tagaaaaaac gttggtatct ttgacaccaa aagctaaaga
     2581 actctgggtc actcgttata cttgtggcaa taatggaggt tggattaagt atcgtatttt
     2641 agaagatgca gacttacttg atattattca atatataaga gcaaaaaaat ctccacctaa
     2701 acaaaattaa cttttatttt ttgtgattta aatatagtgt atataaattc cattctttcc
     2761 atattaaaaa aataatataa aaaatatcta ttagtatatc ttatttagta tgtcttattc
     2821 tactttttac agataattaa aaagttttat cataaataaa tcataactaa acatcctagt
     2881 aaatcaaaat tcatttattc ttttttatta gaatttttga taacagtccc tatacatatg
     2941 ccaattatca ttccaaaaat tagaccataa cacattgtga tacccacatt tcctttagaa
     3001 atacctatag cacaacctat agccattcca agtatcatgc ctataaacat acatataata
     3061 actaaattgt tgtttttatt catagttttt ctccattcaa tatattttaa attgcctagc
     3121 tcgccttctt agcttaaaac aaagaaatat aaattaactt tctttcattc taatatctgc
     3181 ttgtataaat gtcaaacaac acatttcttg tcataacctc ctctttttga agtaagtaca
     3241 atctcccaga cacaataata tcaatttcct aaaaccattt aagtaacttt tatatttaca
     3301 taatatttac aatcataaac tatattgtta ttcatttata catcataaac actccctcct
     3361 aatcattatt atacttaaaa taatgttcat ttataataat atatctaacg cactttaatt
     3421 gaaaaaacta aaactttgcc tttcattccc atatataaat aagtatactt tttgatgctc
     3481 tattacaaat caactaaatt aattgatata aattatcatt ttctgttgaa aataccattt
     3541 acacatgcta tacttaattg cataaatggt aataaatgtc atatgatttt acaaatctat
     3601 taataacaat tattactttt actgtatttt aaagtttttg gactaaatat caagaacttt
     3661 ctaaaataca aagtacaaaa aatttatata agttataaat ttatatgaac tagataagct
     3721 caaacaatat gttatgtata aactgtgtag tattcggtta taaatttata taaattatat
     3781 aagctatata aagtattacc tcaactatat aagctagata tataaattac atacataagc
     3841 tagatatata aattgcatat ataagctata taagaattat ttcaactata taaactacat
     3901 atataaatta catacataag ctagatatat aaactagata tataaactgc atatataagc
     3961 tatataaaaa ttatttcaac tatataaact acacatgtaa aattcatgta aatttactaa
     4021 tataaatcaa ataaaattac taaactctct aaactaattt tgtaaaacat actctatttt
     4081 taacatttgt taccttatac ataagtataa acaaaatcta actaaatgaa aggataatgt
     4141 aaaatgaata ttttaaaaat gaaaaaaaca ggaatatttt taggaggagt actttttggt
     4201 actgtaggtg ttaagatact ttcaagtgac tgtgtaaaaa aaacttgcac tcgtgcaaca
     4261 gcaggagcat tgaaagttaa agattctgtt cttgaaacaa ctacgaaaat tcaagaaaat
     4321 gtagaagata ttttagctga agcaaaagaa atcaacgagc aagaagactt atctacatct
     4381 aaggtaaacg ttgtacaaga tactcttaaa tgcgaacaag aagttcttaa aactgaaact
     4441 ataaaaactg tagaagatac taattctaat aacattaaat gtgaagaaat caaagataac
     4501 gtaattgaag aaaaaaataa tacttgtaca acagaataaa tttaatgatt attatgatta
     4561 ataatatcag gaggaagtaa attgaatttt tatataaaac acgaaataag aggtcgtata
     4621 cgctttgact tacaacttgg caaattaaac aacaagcagt ccgatactct cttatattat
     4681 ttaacttcta taaatggtat taccaaagca aaagtttacg atagaactgg tgatgcagtc
     4741 atttattaca ctggtgaaag agaatacata ataagagaaa tcactaaatt cagttttaac
     4801 aatagcaaaa tagaaaacct agttcctgaa cataccagca gggaattaaa caattattat
     4861 agagacaaat tagcaaataa actgctagtc aaaagtatct ccaaaatatt ttttccaaag
     4921 tccttaagat tttttttaat tggacttcat tctataaaat attttaaaaa aggtctctct
     4981 tccctactaa ataaaaaaat agaaatttct gttttagatg ccactgctat aggtatttct
     5041 atttttagaa aagatatcaa tacggctggt tcagttatgt ttcttcttgg tatcggtgag
     5101 ttattagaag aatggactag aaaaaaatct atagacgact tggctcaaag tatgtcacta
     5161 aacattgaaa aagtatggtt aaaaaaagat gccactgaaa tactagttcc aatttcagag
     5221 ataaaggaag gagatttagt cagtgtaact atgggaaata tgattccatt ggatggagtt
     5281 atagtatcag gagaagcaat ggtcaatcag gcatcactta caggtgaatc ccttgcagtt
     5341 aacaaaaaag aaggctctta catatatgct ggaactggaa tagagcaagg caatattgtt
     5401 atgtgcgtca aggaaaaagc tggtactact agattccaga aaatagtaac tatgattgaa
     5461 gaatctgaaa aactaaaatc ttctgtagag agtaaatttg aacatctagc agatacatta
     5521 gttccataca gctttttagg aagtatatta acctatgcaa ttactagaaa ccctataaaa
     5581 tcactctcta tattgatggt agatttctct tgtgcattaa aactctctat ccctatttca
     5641 gtactatcag ccatgagaga atgtaacaat cacagcattg ctgtaaaagg tggaaaattc
     5701 cttgaaggtg ttgcatgtgc agatactatt gtatttgata agacaggcac attaacaaaa
     5761 gctcaaccaa ctgtttcaga tataattaca ttccaaaatt ataataagaa ggatatgtta
     5821 agacttgcag cttgcttaga agaacacttc ccacactcca tagcaaatgc agtagtttat
     5881 gaagctgaaa aacaagggct tagtcacaaa gaaatgcata cagaggtaga atatattatt
     5941 gctcatggca tttcaacaaa ggtcgatgat aaaaaggttg taataggtag caaacatttt
     6001 gtatttgaag atgagaagtg tactatccca gacggtgagg aggataagta caacaatcta
     6061 tcagatgagt attctcacct atttatggca atatcgggta aattatcagc agttatttgc
     6121 ataaatgacc ctttacgaaa agaagcaaaa tatgtaattt caaatttaag agaatatgga
     6181 ataaaaaaga ttgtgatgat gactggagat agcgaaaaaa cagctaaatc tatagcaagt
     6241 aaagttggtg ttgatgaata ttattcagaa gtcttaccag aagacaaagc caattttgtt
     6301 aaaagtgaaa aactaaaagg tcgcaaagta attatgattg gtgatggaat caatgattct
     6361 cctgctattt cagaatcaga tgtcggaatt gcaatgagtg aaggtgctga aatagcaaga
     6421 gagatttcag atattaccat atcagcagat aatttgaata atttaatcgt tttaaagcag
     6481 attagtaata agcttatgaa aagagttgat ttaagttcta agtttattat cggctttaat
     6541 ttaggtctga ttttacttgg cgtaagtgga tttgttagac caagtacatc tgccttttta
     6601 cacaacgcat ctactgttgg aataagttta aacagtatga caaatttatt ggaaaacacc
     6661 aatacttata actactatta atccccatta tataaaacat atgtatataa aaaatttata
     6721 attcatcttt taaaaagaaa ctgtcttaaa aggagctttt ttctaatcaa aattatataa
     6781 aacatatttt acaaatttta aactcaactt atttctattt tttacaaaaa ggagatgatt
     6841 caaattttca ttttgaaaca tccccttttg taaatttctt taaaacttat atattaatta
     6901 tattttcaac aaataattgt attttcaacc aatctttctt catagcattt taaatcattt
     6961 tgctttagat aattaaattc agactctaca taaataccac agcctaccaa ttcaattgtc
     7021 aaattaggta tattttcctt tagtatacta gtctgctcat aaataattct ttccaataaa
     7081 tcaacttgta atatagaaaa atgcttcata atctccccat cactatctat atctttcttg
     7141 atttcatacc aactctttga caaaattccc ttcaaatcaa tataccctag actcttcaat
     7201 atatctatac tctcgctcca tgcatactta ctaggtttat caaagtatga ttctagtttc
     7261 ttcttctgct catttgtaag tgaatcataa gaaagcttct tattaaaatg tgccttcaaa
     7321 gaaatcttag ccatagaata tggacttcta ccagagttta atcctgcatt tttgaacttt
     7381 tcaattcctt tatcttctac tgaagaccat attgagttta ttgaatctga atcattgtat
     7441 ttaacatcaa gaccatgtgt aatatctata tagttttgtg gaaatacata ttgataatag
     7501 acatcttttt tatcatcata tagggagaag tatccactat catataaatg aatatgcacc
     7561 tttactttta aactatcctc taaaatattt cgtctaaggt tcgggatatt tgcatacatg
     7621 gttacatatt ttatggcatc aaagttttta tttaatggaa cttcaaaact aatttcatct
     7681 cctgtcaaaa gctttttcac ttcttcttca ttacaatcat atagagaata tgtattttca
     7741 aatcccacac gtgatttccc atctaaactg cctgttaatg ctaccttcaa aatatggtta
     7801 tccgtttttt gccattggta agcatcttgg tcaagaaact ctctaatctg tctattttgc
     7861 tctttcgcct gatttataac ctcgttttca tcttctgtaa tacctacatg gcatctattt
     7921 aagatattta ctatattgct tccaaatgga aacgtccata gttgaaaagc tgctgcctca
     7981 acctgacaca aatatctaac ctgatttaat tcaatttgat ggataagttt tttctgatat
     8041 tcattcatct gattaccact acctgacatt tgataaatca attctcccat tgcaagatta
     8101 tcttttttct tatcaatctt atcagtaatg ataatttgag tttccatcat atcctgtact
     8161 tttttaaata ttatagaaat ctcctctgaa ttttccattt ttaagcttcc aaactttgtg
     8221 cttaattcag ttaaaacttc cttcatcttt aatttactta tctcattttc aaaaatatct
     8281 tgatttttaa ctgtactatc gaatttttct acaaatggta taacagattc tttcaaagtt
     8341 ttatctttaa attccaccat ttgagtattc aattcatcta atttcttaat attattttct
     8401 ttatctttaa ttgcctcttt aactacctct attttactct caaaactaga ttttacatta
     8461 agacaattat taacaccact aatcgcagta tttataagag aacaagcaga acttccaata
     8521 ctcaatgcat ttttaatttt agatgtcttt tgctgtcttt caatagcttg tttacttttc
     8581 tctagtgcag ctaacttatc tttttgctgt ttattattgt aatttgcctc caacatacct
     8641 atttcttttt tctggatttt tgtgtaaatt tccttatact cgctttcact taacccctct
     8701 tttgctcttt gaatatcgtc agctttttct gcatagttat catcatctat agacttaact
     8761 ataatctcct taatttggtc aaatttagtt cttctttctt catcaacacc tgccaaatta
     8821 taagtcattt tatctaaagc attttttcta tcttcactta gctgttctcc aagtacaaat
     8881 ctcataccat cagaatccaa tttaaaatct gaaaaatcaa ccatcctgtt agtccattgg
     8941 tattcctctt ctttagctga aagattttcc ttcaaagatt ttgcactgtc aaatgcccct
     9001 agacaagctt gaccaacacc actaaatgca gtcagtaaac tcaatgcgct ttgtattcca
     9061 gaaagaatta actgtgtttt catttgttcc tcaagttttg cctgtttctt ttttagttcc
     9121 tctatgtctt cttgctcctc ttctttcatc tttataattt cttgaatcaa caggttcatc
     9181 tttgcattaa cagaaagcat tttattctca agttcatctt ttaaaacttc tatttgttga
     9241 tttccagatt caatttgagt taggatactt tccttataag tattgaaata tccttctttt
     9301 aactcatctt tctttccttg ttccaattct cttaaggtct tgtccagata atctgtcaaa
     9361 gaacgaatat ctgtaacacg atttcctcca atttcacttt cttcaatcat ctgcacatag
     9421 aatgctgttt tttgttcaag tgcaaataaa atcatactcc attcgctaaa attcttcttt
     9481 tctcctataa aagattctat tctatcagac tgcatcaatt tatttataag gtattcatag
     9541 atttcctttg cctccttata aaattctttc tttatattca tatcatccag agaaagcact
     9601 gctttatttt tcttctcaaa actatagtac ttctgaagta tttctctatt taattgcatc
     9661 tctattttct caactgcatg aaaaatttca taaatatgtg aaagttctaa aatatcagct
     9721 tctaaaattc ccactgcaca caacagtcta agatttctat aacctttaat tccagaaaaa
     9781 ccaaatctct cgaaaaattc atagaagaag gtatctattg acaaagaata ctcctgcttt
     9841 aacaattcct tttcttttaa tgcatctcca gagcgaccgt cttttccctc tgctccgact
     9901 aaacctgaat cttcacgttt gctaattgtc cagtccttat gtttttcata aagagcattt
     9961 aatttctctc catcatctgc tacttggttc tcatttgaag catgattaaa cacatatcta
    10021 tcaaaataat agtaatttct atcactgtca tctattggag cagaggtacc tatatatctt
    10081 attgtttctt catatatacc tctaattcca ccaagacctc ctgcaccacc agctcctcca
    10141 gagcctcctt ttcctgctag accatgattt ccatcttttt tacttatagc accttttaat
    10201 aaagaagctt ttccaaggag ataaacctgt ccagacttgc tcccaagacc tgcatttcca
    10261 ccattacctc ctgtgcctcc agttccacct tgttttcctt tttctgcttt taccacatag
    10321 tattttgtac ctttaacctt agcaaattct tttgactctt ttttagaacc tattttagca
    10381 ttttccccag cttctccagt tatccctgta tctcctcttt ttccatcttg tccatttcca
    10441 ccacgtccac catctacgta aagtttaagt tctcctcttt tttctatttc tttaaaagat
    10501 aataaaatat cacctgaagt acctgcatta ttgccatttc ttccattcat tccgtcgtaa
    10561 atagaacctt ctccaaccaa tttttctgca tccttaccag atacgttgat tgaataaatc
    10621 ccttctggta tacataattt ttctgtaagt agtacaatat tcttacctga gaagacttct
    10681 ttctccatat tacagtcaat tttcaactca caatctccat acatatatat agatgacaaa
    10741 atattaagtt gataaccaga tattatttta gtcaaatccg ataaattcac tttaggtata
    10801 ggaatatata tagaaagtac attttctttc tcatatgatt ttatatatgt acaactttga
    10861 atcttctcta tcctatactc tgcatcttca agctttttgg cttcttcttc cctatattct
    10921 tcttctgcta tccaatattt gatatctttt aatgtatctt ctaaagtatt ttttagcaat
    10981 gattttaaca actcacttag accaacaata tgagtctcac tacagtagct atttaactct
    11041 aaaagaagac gtgtttcttc taatatattc actttctgta cgccatcacc atatgaaagt
    11101 tcttgtacaa actccataag ctctttcata ttattagtcc tactacataa atctgtatac
    11161 atcttatcta attgagtctt tttatccttt agactattta tagaagatag aatggaaata
    11221 gaatcttcca tacctttttt gacactttct atataaggaa ctaaaagttt ttctaggtct
    11281 ttataggtta catcaacggc tttttgtatt tctattttac aagaagcagt aagcatcaat
    11341 cccaaatctg ttttttcaaa tgtttcaggt agatttttca tttcatccaa caacttttct
    11401 ggctgtctat ctagtggatt acataattta atagatacac catctgataa tgctacattt
    11461 aataagtaca ctaattcctt acaaccgtcg taatctttct cttgactatc taggtcttct
    11521 tttgcttttt ccaatatatt ccttattttt tcttctgtta cattaccgat tagtgtttta
    11581 ggtattttat tgataagaaa gaagaaattt tctttatact ttgcataatc accaagcata
    11641 tctttcaaat ttttgcctgt ttctatcaat aatgtatgtc tagagtccag taaggattga
    11701 tagtctaaaa taactaccat ccctttcacc tttgaagatg tagaaatagt ttcccttaca
    11761 agcatagcac cagcaatcat atactctgct ccacggttat caaaaaaacc tgggcaatca
    11821 cataatgaaa aatttctatt tgcaatatct acaacttctg gacataaagt cttagaatat
    11881 acaccatttc caatttcgac agctgaatct tttgcagtta tatatatttg tccagtctct
    11941 tcatccgttt tttcttccat cacgcaacct tttaaataat ttatacaagt actctttcca
    12001 acgcctgaag cacctaagaa taacaaaatt gacttttctt ttaagtcata ttttctaagc
    12061 tcgtctcttc cttcttccaa aaggtttacg atttcactat aaagtttatt tgccatacat
    12121 ccatcccttt ctatatttcg gaacaatagc cttgatattt ttctttaata agattctctt
    12181 ctacctttga aacagctgtt gataacttag tatttggagc attaaatcct ccgtcgtctc
    12241 ctctatctcc atattcagcg tattctcttg ctggtcctgt tttgccttca ttccaacttc
    12301 cgccactaaa ttctatatga tatttaatta tactatctaa cttataatcc caacttgtaa
    12361 aatatcctgt atagtaaaca gtcttatcta taccacttgg agaaaatgaa agtgtattat
    12421 atccgtgata gccaccactt cctccatatc cacctgaacc tggtctacca tttggacctg
    12481 cttctccatc tttcactgtt atatcacaat gattaattat tctatttttt tgttcctctg
    12541 agtctgaaat gaagtaaata ttacctttat taccagcatt tccacctctt ccggcatttc
    12601 caccagctcc acctttgtca ccttttttac cacttgtacc ttttgcaaaa taccctttaa
    12661 acatctgtcc atggaaacta cttgtatatc ctgcatcagc tccattactt ccagaatctc
    12721 ctgtttcacc atttttacca tctattcctt tagaaccatt tcctccattt gcaatcagtg
    12781 ttaaacgata atttctaatt tcttctttaa tatataagta aatattacct gcatctctac
    12841 catcttcacc atttttccca tacatagaac tatctacaag ttctccgctc tttccactta
    12901 cattaatttt tacattacta cctacatcaa gattgttaca agcaagaata atatttttct
    12961 ctgatgcaaa gtccattcca aggttatcat ccatataaat agtatctttg gcaaagaaca
    13021 taaatgtaga aacattgtca tctacttttc tatttttgaa atattccaat acactactaa
    13081 ttactaagtt tggaattaat gctcttattt caataccttt cttatcatta atattaattg
    13141 tttttactat aaatggattt aaaatgtatt taccaataaa ttctctaatt atctcattac
    13201 cttcttctcc atatgcactt aatcttgtta tactgtcttc taactcttca ttttgtgtaa
    13261 ctgtaacatt tgtcaaaatt gaattaaaat cacttacact atatacataa tcctttttat
    13321 ttgcaacttc cattaatttt tctctatttg gtaatgtaaa tacctgtttt tgtacttcat
    13381 aagaaactaa aactttttct aaattctttc ttattataat gaagttttgt tccattatct
    13441 tcttacttgc cttttctata ataaattcca tatgtacaga aggtaataaa atttccccag
    13501 ttccatgttc tgtatattta cttaaagcat caattatatc taagtttcct tttatcttat
    13561 tttgtaattc catgcaacta tcttgtaaga ataagccact ttgtttggct aatacgttga
    13621 taaatctctc tatagtaaga gttgttgtac tttccaacca gtttttaaat acttttacaa
    13681 cctcatcata ttttgctatt tcttgaatca ttgttatact tccaataata ctgataaaat
    13741 cttctgtata cgccttaaca taatcttcta ggctagatac gatattgttt tgaatttctt
    13801 ttatcaacgt atctaaaact aatcttgcat ctgcactaat tggatattgg aatgctgatg
    13861 tcttatcttt aacttcatct aattctagta cttgctttaa taagccttcc ctctcgtctg
    13921 attcaagtgg cttacaaaga tgaatatttt taccattatc ttttagcaat tcttcaaata
    13981 accaagcttt ttcctctata ttactatcta aattttcaca taattgttca tattttcttt
    14041 ttataacatt atatatttgt ttttctgttc ttcctaagaa actttttgaa atgaaaaaag
    14101 ttatactgcc cctaaaattg tctttatgaa gacacatatt taactgttga aacaggtcta
    14161 gtacttttgt aagtctagaa tctaatatat ctgcttctgg tctgaacctc ccatgactaa
    14221 agtcacaggg ttcctgcttc acagaatttt gcatactaaa atatgtctta ctaattctcc
    14281 acaggccatc cccgtagttc ctacggttct tacataagtt atttattttg aattgttatt
    14341 agtcttaatc cttcttttaa tatatttata cttgcattta tatctctatc gtgagtttca
    14401 ttacagctag gacaaatcca ttctcttata cttaagtcct tcacttcctc gtttttatac
    14461 ccacatttat tgcatatttg tgaacttgca aagaatttac ctacctttac aatttgtcta
    14521 ccatgccaat tagccttgta ttttaattga
//
LOCUS       DACTGW010000086        14516 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.086, whole genome shotgun sequence.
ACCESSION   DACTGW010000086 DACTGW010000000
VERSION     DACTGW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14516
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.086"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            274..2916
                     /gene="adhE"
                     /locus_tag="I9573_001992"
                     /gene_synonym="adhC"
     CDS             274..2916
                     /gene="adhE"
                     /locus_tag="I9573_001992"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAT4796612.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPDRVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGNTDQEKVDNLIKAIDELKARVGLPKTIKEAGVEESKFLETLDVMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3276..3617)
                     /locus_tag="I9573_001993"
     CDS             complement(3276..3617)
                     /locus_tag="I9573_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796613.1"
                     /translation="MLLILDNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRINKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3644..4315)
                     /locus_tag="I9573_001994"
     CDS             complement(3644..4315)
                     /locus_tag="I9573_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796614.1"
                     /translation="MPILEILNLGKIYGKKETAVHALKDANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSSGTIKVDGKDICCLSDKKLAKYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKVDEEYIEDIINLLGLSERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4327..6912)
                     /locus_tag="I9573_001995"
     CDS             complement(4327..6912)
                     /locus_tag="I9573_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAT4796615.1"
                     /translation="MKDNLKIALRYIISYKARSLAIALSIILATSLIVGIGTLSRSAQ
                     QAEVDKLKRETGSDHVYFKDINKNQLEYIKSRKDIKNLGITSHYGYTDPNERLAINLE
                     YANKNYLTNQSKLIKGHLPKASNEVVVEKWILNSLGLKPEINQNITFKLYQKEKPETF
                     KVVGILEDRPIEKNKGTCEIFLNLNESKLDKFSYAYIEFNNGIDINTSIDNIVKNTML
                     DENSVGKNKMLIESTRENGTLDNSSKYTAITMSLFSGIVIYSIYVISIYQRIQEYGIL
                     RAIGSTNFKIFKFMFYELFILALIAIPIGICTGIGGAQIFNRTAGNIQFEGNVTVTPF
                     VIPDKIILLSIGSIILTILIISFFTYLKIRRISPIDSIRKTFGTDKNINKVNSLISKL
                     TLNISVTKSISAKNIFRNKKGFIIIILSMSLGGIMIIKENYKYSFSDIQNKNGQEETY
                     MNADFILTNFFLKLNQSNKADSFKDIKGLNDNQIDKIKNINGIDKVKTASILDTRIEV
                     DKLNGLDYYNIINSTPYYKDFPLFIKNKNTGKYTMKQKLRGYNDEMINSLEKYLVSGS
                     INLERMKKENLAILYVPQVSKTNKYKLSYTPGTETPAVDIKVGDTIKVKYPKGEIDQD
                     LYIKLKDNYEYLEYEFKVGAIVSYPFADNYLYSGDQGIDVITSNDYLKKLTGVDKYNV
                     VYVDINKNANVKKINTLLGEIGSESPGTTTANMLMEKENFDKMTARALTYAYGIVAVM
                     FIISVFNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGILSSVITIVV
                     GLIIQFRMYYTYNFVSYGLGFSIDYKIYILVVLANIIVGILATYIPLRKINKISIVEA
                     INITE"
     gene            7120..8055
                     /locus_tag="I9573_001996"
     CDS             7120..8055
                     /locus_tag="I9573_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAT4796616.1"
                     /translation="MENNFIFPIVILTLFIFSNLIMNKYRFYKELDILKRGILTFFVS
                     CFVWILFMEGIEYIQLSLGYNFLLEGVLNIILFLILIILSFYIGFKFIRLLDNNNKQE
                     YKLQKLDSKINHKDNIIVKLRSQKHDYLKHLQVVYSLLNTGLAEDAKEYITNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKNISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSAIQKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFLDGFSTKEGQDRGF
                     GLYLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            8074..8811
                     /locus_tag="I9573_001997"
     CDS             8074..8811
                     /locus_tag="I9573_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4796617.1"
                     /translation="MIKVLICDDDKIVRDDIANIVKESGYVDYVKKVKDGIEAIEFIK
                     SEKIDLLIIDIDMPYKNGIDSAKEILSIDKDIHIVFVTGFADYSLESFKVHPIDFIVK
                     PFKKEKILDSINIAIDHINSHKIAKNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLYTRDEDTIKFYENFDDLKKRLPPNFFMTHRQYIVNLRLIHKVIPINKSSLEIKF
                     INFQESATLNKSLEKDFLYRFYRVKRF"
     gene            complement(8971..14028)
                     /locus_tag="I9573_001998"
     CDS             complement(8971..14028)
                     /locus_tag="I9573_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAT4796618.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVTSALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNSDRQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTSEDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKFKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGIDYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDESGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQLVQPFKQVFREYYRINSDEISEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNINFEGSHV
                     LITGNLGEYSVHMGSGIVHKKGMGALNILPVHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDNKIKDPSILSNL"
     gene            complement(14053..14472)
                     /locus_tag="I9573_001999"
     CDS             complement(14053..14472)
                     /locus_tag="I9573_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796619.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
BASE COUNT         5007 a         2074 c         2087 g         5348 t
ORIGIN      
        1 ttttatttag ttgaagtaaa ttattcaaat gaaatatatc tcatttagat aaaaaatttt
       61 agtaaaagta aaaaaaataa tatatttgtt aaaaaataaa ttgacaaaat attcagaaaa
      121 aaacttcttg acctaagaga gtaaaataaa taaaatctaa ggtatagaga tgaaatctat
      181 aaaattattt taagtataag ttaaaaatat agatggtgca tttaagattt ataaaaatta
      241 gagatgcata attattttag ggaggttttt agtatggaga aaaaagaaaa ggtagtagaa
      301 aaatcaaatg ttgaagtatg tagtcctgaa attgtcaaca gtgttgaaac attaagaatg
      361 agattagaag aaattagatt ggcacaaaaa gaatttgcaa catttacgca agaacaggta
      421 gataagattt tcttagctgc ttcaactgca gctaatcaac aaagaatacc tctagcaaaa
      481 atggcagtgg aagagactgg tatgggtatt gttgaagata aggttataaa aaaccatttt
      541 gcctcagaat atatctataa tgcttataaa gatactaaaa cttgtggtgt catagaaaaa
      601 gatgaagcat ttggttttac aagaattgct gagccagtag gtgttttatc agcagttata
      661 ccaacaacta atcctacatc aactgcaata ttcaaatccc taatagcact aaaaactaga
      721 aatggtataa ttttctctcc tcatccaaga gctaaaaact gtactataga agcagctaga
      781 gttgtacatg atgcggcagt taaggcagga gcaccaaaag gacttatagg ttgggtagat
      841 gttccttcga ttgaattaac taatgttgtc atggcagagg cagaccttat attagctact
      901 ggtggacctg gaatggttaa atctgcatac tcttcaggta aaccagctgt tggagttggg
      961 cctggaaatg tacctgcaat aatagatgag agtgcagata taaaaatggc tgtaagttca
     1021 atcttagtat caaaatcatt cgataatggt atgatatgtg cttccgagca agctgtaata
     1081 gtaccagaaa aaatatatga agaagttaaa aaagaattta aataccgtgg agcacatttc
     1141 ctaaataaag aagaaactga aaaagtagga aaagtagtaa taattgatgg ttcattaaat
     1201 gccagaatag ttggacaacc agcacatgta atagccaaaa tggcggatgt agaagttcca
     1261 aaaacagcta gaataataat tggtgaagta gaatctgttg aattaaatga accttttgca
     1321 catgaaaaac tttctcctgt acttgcaatg tacaaatcta aaagttttga agatgcagtt
     1381 gctaaagctg aaaaattagt tgcagatggt ggatatggcc atacatcttc gttatatgca
     1441 gactctataa atcatccaga tagagtagag aagtttgtaa atgctatgaa aacttgtaga
     1501 gtattagtta atactccatc gtcacaaggt ggtataggtg acttatataa ctttaaatta
     1561 gcaccatctc ttactcttgg ttgtggttct tggggtggaa actcagtatc tgaaaacgtg
     1621 ggagtgaaac atttacttaa tatcaagaca gtagctgaaa ggagagagaa tatgctttgg
     1681 tttagagcac cagaaaaggt ttatttcaaa aaaggttgtt taggtgtagc agcaagagaa
     1741 tttaaagatg ttatggataa aaagaaagcc tttatagtaa cagattcttt cctttacaat
     1801 aatggataca ctaagaagct aactgatttg ttggatgaga tgggaattaa gcatactaca
     1861 ttctttgatg ttgctccaga cccaacatta gcttgtgcaa gagaaggagc taaggctatg
     1921 gctgatttcc aaccagattt aataatagct gtaggtggag gtagcgcaat ggatgctggt
     1981 aagataatgt gggtaatgta tgagcatcca gaggtagact tccaagattt ggctatgaga
     2041 tttatggata taagaaaaag agtatatgta tttcctaaga tgggtgaaaa agcgtatttt
     2101 gctgctatcc caacttcagc aggaactggt tcagaagtaa ctccatttgc agttataact
     2161 gaccaagatt caggtgtaaa gtacccatta gcagattatg aattaatgcc aaacatggct
     2221 ataattgatg ctgatatgat gatggagatg ccaccaagat taacagcagc atcaggtgtt
     2281 gatgctttaa cacatgcatt agaagcatat gtatctatgt taagaactga accagctgac
     2341 ggtctagcac tacaagcagg taaaataata tttgaatatc tacctcgtgc atacaaaaat
     2401 ggaaagaatg ataaagaagc aagagaaaaa atggcaatgg catctactat ggcaggaatg
     2461 tcttttgcaa atgccttctt aggaatatgt cactctttag cacataaatt aggtgcattc
     2521 caccatgttc aacacggtgt tgcaaatgca ttattaataa atgaagtaat taagttcaat
     2581 tgtgcagaag ctccaaataa gatgggtgca ttctcacaat acagatatcc tgattgtata
     2641 caaagatatg ctgaatttgc ttcttttgca ggaatcaagg gaaatacaga ccaagaaaaa
     2701 gtagataacc ttataaaagc aatagatgaa ttaaaggcaa gagtaggact tcctaaaact
     2761 ataaaagagg ctggagtaga agaatctaaa ttcttagaaa cattggatgt tatggttgaa
     2821 caagcttttg atgaccaatg tacaggtgct aatcctagat acccattgat gagtgaatta
     2881 aaagagatat atctaaaagt ttattatggt aaataatatt cattgtacaa cataggttct
     2941 attgggtttg tatataaatt attagatttg aatataaata gagttttatt agagaaacgt
     3001 agaagtaact ataaataata gattataact taaattctga atggaaatga taaaaagtca
     3061 gtaatttaat ggtaatttcg aatatgtgta tttaatcaat taactttaaa attagtcgga
     3121 aaattttttt attacatata attttaaagc ataatatata aaaaacttta tgaaaaaatt
     3181 ataaaaatat tcagaaaatt ctattgacta atttatttat catgatataa taaatttaga
     3241 aaagaaaaaa attggggcct attaaggccc ccttactatt ttttgataga tttatttaaa
     3301 attactttct cattttgatt ttttatattt attatagagt cagtataatc cacttcaaag
     3361 ttggcaaaag cagagaataa atctatatct cttaacaaat gaattgtcat acattgattg
     3421 ttcttattta tccttagaat caatgctctt acaaatggaa attgtattct agaattaaat
     3481 aagttagaac catcagtatc tatatctaag ctacgataat taatatctat aaatatatct
     3541 acatcatcat tttcttctct tattattctg tagtcaaaat ctaagtttaa atttaaatta
     3601 tctaaaatca ataacattta aatcctcttt tctataaata gttttagagt gttaatttaa
     3661 gctctccatc tattatagat ataattctat ctgcttttct agcaatattt tcattatgtg
     3721 ttattattat aagtgtttgg ttatattttt ttatagaact ttttagtaaa tccatgactt
     3781 cttccgtagt tttactatcc aaatttcctg ttggttcatc tgctaggatt atacttggtt
     3841 ttgcagataa agcgcgtgca atagcaaccc tttgttgttg accaccagat aattgatttg
     3901 gaagatgatt tttcctctca ctaagaccca ataaatttat aatgtcttcg atgtattctt
     3961 catcaacttt tttattgtct aactttagtg gtaattctat attttctttt acatttaata
     4021 ctggaattag gttatactgt tgaaaaacaa agcctacttt ttgtcttcta tattttgcta
     4081 attttttatc tgaaaggcaa catatatctt taccatcgac ttttatagtt ccactacttg
     4141 gtctctcaag acctcctact aaatgtagaa atgtgctttt tccacttcca cttgggccaa
     4201 ttatcgcaac aaattcaccc ttatttattt ttaaatttgc atcctttagt gcatgtacag
     4261 ctgtttcctt ttttccataa atcttaccta agttaagaat ctctaatata ggcataatta
     4321 atcctcctat tctgtaatgt ttatagcttc aactatacta attttattta ttttccttaa
     4381 tggaatatat gtagctagaa tacctacaat tatgtttgct aaaacaacta ggatatatat
     4441 tttgtaatct attgaaaatc ccaatccata tgatacaaaa ttatacgtgt aatacattct
     4501 aaactgtatt ataagcccta ccacaatggt aattacactt gagagtattc catataatat
     4561 tccctcatag agtatcatat ttttaaatcc tctctcagag ataccaatag ctcttaacat
     4621 tccaaattcg ctagttcttg aagttaagtt ataacttatg ttatttatta tattaaacac
     4681 actaattata aacattactg ctactattcc ataagcataa gtcagagccc tagctgtcat
     4741 cttatcaaag ttttcttttt ccattagcat attagctgtt gtagttccag gagattcact
     4801 tcctatttca cctaagagtg tatttatctt ttttacattt gcatttttat taatatctac
     4861 ataaactaca ttatatttat ctacaccagt tagttttttt agatagtcgt tacttgttat
     4921 tacatctata ccctgatctc cagaatatag gtaattatct gcaaaaggat aacttacaat
     4981 agctccaact ttaaactcat attctagata ttcataatta tcttttagtt ttatataaag
     5041 atcttgatct atctcacctt ttggatattt gacctttata gtatctccta cttttatatc
     5101 tacagcaggc gtttctgtac caggagtata acttagcttg tatttatttg ttttagaaac
     5161 ttgtggtacg tataatatag cgaggttttc ttttttcatt ctttccagat ttatactccc
     5221 actcactaag tatttttcta atgaatttat catctcatca ttgtaacctc ttaacttttg
     5281 tttcattgta tattttccag tattcttatt ttttatgaat agaggaaaat ccttataata
     5341 aggcgttgaa ttaataatat tgtagtaatc taatccattt aatttatcta cttctattct
     5401 tgtatctaag atgctggcag ttttgacttt gtcaattcca tttatatttt ttattttatc
     5461 tatctgatta tcattaagtc cttttatatc cttgaaagaa tcagctttat tagattgatt
     5521 tagttttaag aaaaaatttg ttagaataaa atctgcattc atatatgttt cttcttgacc
     5581 atttttattt tgtatatctg aaaaagaata tttataattc tcctttatga tcattatacc
     5641 tcctagactc atggataata taattataat gaagcccttt ttattcctaa agatattttt
     5701 agcagatata gattttgtta ctgatatatt taaagttaat tttgatatca aagaattgac
     5761 cttatttata tttttatcag ttccaaaggt ttttcttata gagtcaatag gagaaattct
     5821 tcttatcttt aaatatgtaa agaaacttat aattagtatt gtcagtatta tacttccaat
     5881 tgataaaagt attattttat ctggaataac aaaaggggta actgttacat taccttcaaa
     5941 ttgaatattt cctgcagtcc tattaaatat ttgtgcacca ccaatacctg tgcatatacc
     6001 gataggtatt gctatcaaag caagtatgaa aagttcataa aacataaact tgaaaatctt
     6061 gaagttagtg gatccaatag ctcttaagat accatattcc tgtattcttt gatatatcga
     6121 tataacatag atactatata ttacaatacc agaaaatagg ctcatagtaa ttgcagtgta
     6181 tttacttgag ttatctaaag taccattttc cctagtagat tctataagca ttttgttttt
     6241 tccaacacta ttctcatcca acatagtatt ttttactata ttatctatgc ttgtatttat
     6301 atcaatgcca ttattaaatt caatataagc ataagaaaat ttatccaatt ttgattcatt
     6361 gagatttaaa aaaatttcac atgtcccttt atttttctca ataggtctat cttcaagtat
     6421 tcctacaact ttaaatgtct ctggtttttc tttttgatat aatttaaaag taatgttctg
     6481 atttatttct ggttttaaac ccagactatt taaaatccat ttctctacga ccacctcatt
     6541 actagctttt ggaaggtgac cttttataag tttggattga ttagtcaagt aatttttatt
     6601 tgcatattct aggtttatag ctaatctttc attaggatct gtatagccat agtgagatgt
     6661 tatacctaaa tttttaatat cttttctaga ctttatgtat tctaattgat ttttatttat
     6721 atctttaaag tatacatgat cacttccagt ttctcttttt aatttatcta cctctgcttg
     6781 ttgagcactt cttgagaggg taccaatacc aacaataagt gatgtagcaa gaattatact
     6841 tagagcaatt gctaaagacc ttgctttata acttattatg tatcttagtg ctatttttaa
     6901 attatctttc atatttacca cttcctttct tagctaaatc aattatatgt ttatttaaaa
     6961 tatatatcaa agtaagtatc ttcaagtttt gagtacttaa ttgtacaaat ttatatgtgt
     7021 atttgcaagt ttaattaatg tacatgtaaa ttttgacttt ttacataatt tttctaaaat
     7081 gatataatcc tcatataaag tgtagatggg aggggaatta tggagaataa ttttatattt
     7141 cctatagtaa tactaacttt gtttatattt agtaatctta taatgaataa atacaggttt
     7201 tataaagaat tagacatatt aaaaagagga atattaacgt tttttgtatc gtgttttgta
     7261 tggattttat tcatggaggg tattgagtat atacaattaa gtttaggata taatttttta
     7321 cttgaaggag tattaaatat tatacttttt ttaattttaa ttattttaag cttttatata
     7381 ggatttaaat ttataagatt actagacaat aacaataaac aagagtataa gctacaaaaa
     7441 ttagacagta aaataaatca taaggacaat ataatcgtaa aattaagaag tcaaaaacat
     7501 gactatttaa aacatttaca agtagtatat tctctattaa atactgggtt agctgaagat
     7561 gcaaaagaat atataacgaa tatatcaaat agctttaaat caaaaaattc aagatatggg
     7621 aaagtatctt atttagatgc tatagcttcc ttaaaatatg aggaatgtct tgaaaagaac
     7681 atatcttttg atgtatatat ccaagatttt atggataatt tagagattga acctaatgat
     7741 atgagttcag tgctattaaa cataatagac aatgcaatag aatctttgtc tgctatacaa
     7801 aaggaaaaga agtatataag attacatgca tatgaagatg aatttcagta tattatagcc
     7861 ataaaaaata atggtcctaa aatacataat gtcgaaagta tatttttgga tggatttagc
     7921 actaaagaag ggcaagacag aggttttggg ttatatctag ttaaaaatat actagataag
     7981 tatgattata gtatacttgt aaatagtgac gattttttaa ctgaatttgt aattttaata
     8041 ccaaaatatt attagttagg aggagagaag tctgtgataa aagttttgat ttgtgatgat
     8101 gataaaattg taagagatga tatagcaaat atagttaaag agtccggata tgtagattat
     8161 gttaagaaag tcaaggatgg tatagaagca attgaattta taaaaagtga gaaaatagac
     8221 ttattgatta ttgacattga tatgccatat aaaaatggga ttgattctgc caaagaaatt
     8281 ctttctatag ataaagatat acatatagtt tttgtaacag gttttgctga ctatagtctt
     8341 gagagcttta aagtacatcc tatagatttt atagttaagc cgtttaaaaa agaaaaaata
     8401 cttgattcaa ttaatatagc aatagaccac attaactctc ataaaattgc aaaaaataat
     8461 tttatagaag atactctttt tgtatataaa ataaggaaac agatacatat gattaatttt
     8521 gatgatatag tcatgtttga gaaaaattca agagctatta atttatatac tagagatgaa
     8581 gatacgataa aattttatga gaactttgat gatttaaaaa aacgcctacc tcctaatttc
     8641 tttatgactc atagacaata catagtaaat ttaagactta tacataaggt tatacctata
     8701 aataagtctt ctttagagat aaaatttata aattttcaag aaagtgctac tttaaataaa
     8761 tctttagaaa aagattttct atacagattt tatagggtta aaaggtttta atgaagaaaa
     8821 aaattttcag tgatatacaa tatgtatgat gaagatatca tataaatttg ctatagttat
     8881 aaattaattc gtataaaata taatataaaa catattggac tttcatggaa aattagaaag
     8941 cccaatatgt tttagagata aatcttatat ttataagttg cttaagatac tagggtcttt
     9001 aatcttatta tcttcagcaa gtaataagat tttagacata atctcagcag tcttagggtc
     9061 ttcatcagcg aatggaagga atatcttacc tctactttga gaatgtacag gaagtatatt
     9121 caaagcaccc atcccttttt tatgtacaat accagaaccc atatgcacag aatactcacc
     9181 taaattacct gtgataagaa catgagagcc ttcaaaattg atattgttaa tttttaataa
     9241 taataaaagt tctttagcaa ttgctatacg catctctaca gtagaatggc tagtttcagg
     9301 gtcaacacca ccagcatgag cgacacttac aacaaggtct atatcacgca taacttctga
     9361 gaatagaatt ggaggtatag aagctaattc taaactttta taagtttttc tatcaaaaaa
     9421 ttctactgtt tcaatagtag gaggctctat atctgctggt gaaaaccaat ctgcaagtgc
     9481 aaacatcttt gccactatat tttctttata aaatactttc tgtaatccct cttcataatc
     9541 gacagtccaa agctgatttc taagaagtgc tacagttttc tttggctgaa tttggtgacc
     9601 agcatatctg cgagatatag ttttttcaga tatttcatca gaatttattc tataatactc
     9661 acggaatact tgcttaaatg gttgaacaag ttgcttacta aataaatcat gttggaactc
     9721 actccactta ccagattcaa acaaatgaac tggatgtgca agttttaatt tattttttgc
     9781 tgtcaactta taagtttcac cttctatatt gcaaataaag aacttagact tatcattata
     9841 aacatatcca atattatcat cacataccca tactagattt ttaatcaaag ggcagataac
     9901 tggattttta tagatattgg caatttcttt aactgtaaat actatttcat ttttcatagc
     9961 ttgttctaaa ctctgttttg cacgagaata ctgactcttt aaatcttttt ggacagattt
    10021 aagttcaagt acatatggat gtttagcaat agcttttggt atactcttta aagacttgtt
    10081 gcctttttga actttagtag atgcttttcc gctttcatca acatctatcc aaattgaagt
    10141 accatcaata tcttttggtt taaatagatg agcaacttca tttagttttt cagtctcaag
    10201 agcccacatc atacgattta catctccaaa tccagcactt ctagctagat tttctagcgc
    10261 tatagtacaa gcttttgatt cactttcacg acgttgtgca ccaaattgct tgctttcttt
    10321 agcaaacttt tgaataaatt cataacggaa agttgcttct tttaggtctc cttttttcat
    10381 aggaagtaaa gagaaacttc tcaatttatc ttgatttcta tttgtttcaa tttctttcat
    10441 caacatgtct ttatcaagct taccaagtgt agcatcagca tagagttgtg cacgtctatg
    10501 agagttacca ccactagtta tatatttagc tgacttgtaa agtatattga atctttcttc
    10561 cccaatagtt ttatatgaat ctaagaacca atctacatca aatgcaccat caatgaaact
    10621 acgtggtgaa ataggggaga atagagcaac ctctgattct ttttgtgcag agaagccttc
    10681 atttatatgt gcatggaagt accaaattgt acttttcaga cccttccagt ttaaatattt
    10741 ttcaattatg tttacccatt gaggggcata catagcagat tctattaatc ttaactcagt
    10801 aatgtcggta tttttaagta aactttttaa tttttttgaa tcatcttctt tagtaggata
    10861 gcaattttta agtagagaac ttaaaacatc tttttttgaa taattgctac catatgaata
    10921 tcctctctgg aaagtttctt taccaagagc gaccaaaata tttacaaaat aatctatacc
    10981 ttcaaagtat tgtatattat tagctagatg agacacttct gttggcatat cacctctacg
    11041 tgattcaatt tctataattc ggtttgtaac agtttttaaa ttatctttaa gccaagggta
    11101 cttttctaat gtgtcaccag cccatctagg atttgttaat gttcttatta tatttgatgc
    11161 gttgtatcca cttatcattt ctctattaac ttgctcttca gtcattaaac ctaattcatg
    11221 tgcacgagca aacacattta tgctagtata gaaaggaaac ttatattcta gcaactgata
    11281 atttgaaaac gtagtgtaga aaaacttagt aaaagaaata tcatcataaa taaacgaatg
    11341 tgctttatca agccaatagt ttaaaatagg ggcatttaaa gcagggcagt attcattttg
    11401 tctgtaatag tttttagtaa attcaactct ttgtttttta cattgttcta tagtcaataa
    11461 gtttctaagt ttatagtata tttctaaaca atagttaaat atttctttat ggtcaaactc
    11521 atttagtaaa actcttaaaa gttttcttgt aagtccatta aatttaaatt tttcaagttt
    11581 tgtatatatt ttattttgtt ttttagagtc aaggacattt gaatatatag atgaaaacca
    11641 acttttttct gtttcattat atccacatgt acttatacta taaaataaga tatcaagtat
    11701 taatttgtaa tctaagtcaa gctttttact agtttctctc caaagttctg ccaatggata
    11761 attatcaaga gtatcattag aaactttata tttaatagga gtcaagttat aagtagaata
    11821 acctaaaagc actttgtcta cattacctga ccatctttca acttcatatt cataatctgc
    11881 attttcagta ataagctcat caaatttttc ataaactttt attattttat ttatatccgt
    11941 atcgagaatt ttttttgctt ctttttttgt tgctacagaa acagaatcct cactagtaga
    12001 taaacttata tttgaaataa ttttattaag ctttgatgag ctattagaat catctttaaa
    12061 atcaaattgt tctggaattt gaactttgtt ttcagggtta tatattccaa aaccattttc
    12121 tttgcagtaa tcattttttg ctttacctaa caaattatca attaatattt gaacttgaga
    12181 tgtattcttt ttagatttat caatatcctc tataagtaaa ataagcttat tatatagttc
    12241 ttgtctatca gaattgtctt ttaataagtt tacaatttca agacctccaa ggcatttttg
    12301 agaatttgaa gattttatca gtgaagaagc aatattttcc aaatttgaat catcttgttc
    12361 aagtaaaatt tttattactg tttgtctaat agagcttgtc tttaaagaca atagattggt
    12421 tatcttatct aattctacat ctgttagctc aagctttcta agtaaactca ataccgtttc
    12481 tctattggaa acactcttat cacttaaaca ttccaataaa aattcacgtt gtctatctgt
    12541 ctttggttca tttacaaaat tgactataag tgaagctctt aaatctgaac tcataagatg
    12601 acaattttcc ataagcacat ctataaattg agcattcata tcgtatgctg ttaaagtcat
    12661 catacattta agtatttcat gagaagaaat ttcaatccaa gtccatggaa atacacttcc
    12721 tgaaaatgat tttgtctttg aatttaaaat ttctaaagta tgttttaaac tttcaaattg
    12781 ttcttttcta gcatcaaagt ctttgtcaat aggagtgttt gacaagtcaa aattagtttc
    12841 tctgttttgc caactatagt agtagccaac agaaccttct acatagaaat ttgtaattac
    12901 ccatgctagt atctctaaat catcaccctt aatgttttta gatgctatag aatgtttaaa
    12961 atatgtactt tctgtatgcg ataaaaagta aagagctaca agttttttat atttttcctg
    13021 acaagaaagt aagttttcaa taagagagta agagtcatat atgttgtaag ttgcagtagc
    13081 ccataaggca aaataaattt caagattatc attgctatta attgattcta tacggaactt
    13141 ttcatcagtc aatgatttat atgaaagttc taaacattta cgtattattc caggtttttg
    13201 gtctgaaaaa ccaagtcctg tccatgtatc caaggctctt ataacagaac taaatcgctc
    13261 taaattgttg tccaaaatag tttttaacat gtatattgat gcagaaattg taccctcatc
    13321 taaactctct acaatttgct gtctaagtcc ttcttgttgt ttggctgcca aaagaagttt
    13381 agaaagcatt tcatagcatt tatcactatg actcttcatg ataccaatta tcatttgtct
    13441 tgtaagtaga gcagtttgat tttctccaaa tattacttct tctaaggcac ttgttacttt
    13501 ttgatttcct ttgtctattt ctagtgctat tttatcactc attatagagt catagtacaa
    13561 gaccttattt ggaagagtaa gataatctat aactgaaaaa tcataataaa ttaaatcaac
    13621 atagtttgaa ataaacctta taatacctct aaaataatcc acaagtttat ttgagcgata
    13681 ccctttacga taatatcctc ctgaatatgt ataatccatc tttcttttaa ttaattggca
    13741 aagttcttct acttgttcag aagtaaatat taattctaat gcatttttta aaggtccaga
    13801 aaggagctcg tccatatttt taatttcgta gtagtggcaa ttgtagtatt tctttatcat
    13861 atttaagaat ttgtcctcag gttctctata atagtatgaa ctattttcta tgcagtccca
    13921 aagagatttt gctaagtcgt agtactgttt tgaagaagat ttagtaaaat tatttttaaa
    13981 attttcaatt attttaggat ttttttctac tctagtacgt tctaacattt atgtacctcc
    14041 aaattattta ttttaaaaca ttcttccagc aagcaaggta agtcttataa aagtaaatat
    14101 atctccactt ttcagattta taggggaatc gagtttttca ataacatctt catttataag
    14161 cactttgtag attccatctt caaatgcctg taacgctact tctattgcct tttctacaga
    14221 ttgtttattc tcattgtaaa tagagccaaa tccaactttt ccaatatcag cttgagattc
    14281 aatttcttca ttagttaaga agtttattat tgatttttct gggattttct cattatattc
    14341 aagaacattt ttagatacaa tttcagagat agcttctttt aggcaattga tgttgtcaga
    14401 tacatcaaaa ggggtaagtt ctaacatagg tttgcgtttg ccaacagcct taactctaaa
    14461 ataaatttgc ataaaatcac ctcgcaaaat attaattatg tatttagaaa tttaaa
//
LOCUS       DACTGW010000087        14428 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.087, whole genome shotgun sequence.
ACCESSION   DACTGW010000087 DACTGW010000000
VERSION     DACTGW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.087"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..2000)
                     /locus_tag="I9573_002000"
     CDS             complement(<1..2000)
                     /locus_tag="I9573_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4796620.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGEVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKRAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGI"
     gene            2390..3058
                     /locus_tag="I9573_002001"
     CDS             2390..3058
                     /locus_tag="I9573_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796621.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLASNFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            3060..3545
                     /locus_tag="I9573_002002"
     CDS             3060..3545
                     /locus_tag="I9573_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796622.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            3606..4727
                     /locus_tag="I9573_002003"
     CDS             3606..4727
                     /locus_tag="I9573_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4796623.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMQFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            5008..6198
                     /locus_tag="I9573_002004"
     CDS             5008..6198
                     /locus_tag="I9573_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAT4796624.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDIVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLVRKGFKH
                     TPLQSLCPEYKDKIITCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     gene            6646..7647
                     /locus_tag="I9573_002005"
     CDS             6646..7647
                     /locus_tag="I9573_002005"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAT4796625.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            7751..8140
                     /locus_tag="I9573_002006"
     CDS             7751..8140
                     /locus_tag="I9573_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HAT4796626.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIIKKDKHSNIIENVEFSIKDEEE"
     gene            8301..9509
                     /locus_tag="I9573_002007"
     CDS             8301..9509
                     /locus_tag="I9573_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAT4796627.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(9596..10672)
                     /locus_tag="I9573_002008"
     CDS             complement(9596..10672)
                     /locus_tag="I9573_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAT4796628.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            10901..11428
                     /gene="hpt"
                     /locus_tag="I9573_002009"
     CDS             10901..11428
                     /gene="hpt"
                     /locus_tag="I9573_002009"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAT4796629.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            11517..12449
                     /locus_tag="I9573_002010"
     CDS             11517..12449
                     /locus_tag="I9573_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAT4796630.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIVGWMLGIFFTVIF
                     IKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            12511..13737
                     /locus_tag="I9573_002011"
     CDS             12511..13737
                     /locus_tag="I9573_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAT4796631.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINANTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            13821..14042
                     /locus_tag="I9573_002012"
     CDS             13821..14042
                     /locus_tag="I9573_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HAT4796632.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
BASE COUNT         5462 a         1584 c         2463 g         4919 t
ORIGIN      
        1 tttatgcctt tctcagttat tgatgagcct tttcttcctt tactagaaat taagtatcca
       61 ttgcgtgtca aatattctag catatttctt acttcgtatt ctgtgacatt atattccata
      121 aatttcatta actttgtcct ccccattggt ttcatgtgca atgattttag gatagaaatg
      181 catatgtttt tgtcatattg gtttaaatca ttgaatatag acatattact tgacatatga
      241 ttattattta attttgaaga tatgttttga ggtaaatcat ttattgtaag ataattggaa
      301 cccatgtaag ccatatattc aacacaattt ctcagttctc taatattccc catccaagtg
      361 tatgaattta aagcttctaa aagactctta tcgagtttta tgtttttata gggaagttct
      421 tttaaaaatg attctaaaag aagaggtata tcttctaatc tctttctaag aggaggtaaa
      481 tctaaagtga acatacttat cctataaaat aaatccatcc taaatttatt ttctataatt
      541 aatttttcta aatctttatt tgtggcagct aatattctca catcaattgg aataatactt
      601 gtaccaccaa tttttctaat ttctttttct tgtaagactc ttaaaagttt aacctgtaaa
      661 tcatgtggca tatctcctat ctcatctaaa aatatagtgc cagtatgagc taattcgaat
      721 aatccttttt ttccaccttt ttttgctcca gtaaaagaac catcttcata tccaaatagt
      781 tcactttcaa gtaattcact tggtaatgaa gcacaattta ttgctacaaa aggtttattt
      841 tttctatttg aatgattgtg tattgactga gtaaaggctt ctttacctgt tcctgtttca
      901 cctgttatta atataggatt atctgtaaga gccatttttt ttgccctttt aatacaatct
      961 tttataatag aactatttcc aataatatta ttaaatttat atttagctac ataacctctt
     1021 ttgacagaat cacttcttat tttttgttct aatatttcta tattgtttac atcttttaat
     1081 gtaattaagc aaccctctat atttttataa agataaaatg gttttttaga aagtatgaat
     1141 tttttatcta tttcgtctat gtatataatc atattatcga tatttaagtt ctttgtaatt
     1201 ttatctaaga aactctttgg catatattcc attaaggaag gtgattttat aagtttttca
     1261 tctagttcca acatattaag taaagactta tttacaaaag taaccttgtt aaaggtgttg
     1321 aaaaatatta gaccatcacc tatttcatca atggtcatgt aaagtatagt ttttaattta
     1381 gatatattat ctaatgacgt atttaagaaa tcatgactta gtgtctcctt ggacaatttg
     1441 tatagttttt ctataaattt ctcatttttt agttttaata ctactaataa tgacatatat
     1501 gtatctaatg agatttttct ccaacctaaa tcaacaatat tttttatgta ttttggtata
     1561 aatctaatct gacctggtgt tatagctgta tctaaatcat atatttcatc acaatctgga
     1621 tatgtcggta caaaatctat atgatttata ccaattttat atagataagt agccatctca
     1681 aatgctgaag ttttattggt tgtcataagt aacgattttg tacctggagg tagtttcatc
     1741 attttttcat atccaagttt tgaaagtgct ttcgttccat gaataatttc aatatcttcc
     1801 ttaaaatgtt ttattaaaaa attactagtt aaaggtgata gagaaagcac tacctctccc
     1861 tctaaatttt cttttattcc atcaagtata ttttgtgttt ctatgattat ttcgtctcca
     1921 aaaaagtttg ttaattcttc tttgtagtgg tcactattta atttatcata tgttataagg
     1981 attaacttct ttttatccat tttatttctc ctaacaaaat agtatttatt tttaaagtat
     2041 ttaaagtata aggtaagtta tattatacaa caaatagtag tataaattta tatatttgat
     2101 taaataatag gtttttttag gattttatcc tataaaaacc tattttaaaa ttatttttgc
     2161 aagataagga aaatagcttt ttatattgta atgtatattt taaatagtta ataaaaaaat
     2221 attatatttt taaacttgaa aagtaagtaa ttataagcta tttaatcgct ggaataaatt
     2281 tacaataaaa aaataaatac aaaggcttta tcattaaggt attggcataa gttttgctta
     2341 ttaaacaagt atactaataa aattcattta aacaggaggg agaatttata tgagtaaatt
     2401 agaatcaaca acagaaaaag ttgaggtaaa atggtatggg tatgtagcat taatattggg
     2461 agcattattt ttttcaggaa tatttaaaga tgcaccagga ccattaaaag tattggattt
     2521 taacaatgta ttaggttctt ttggaacatt gggaacaatt aatgatggtg taggtacatt
     2581 agcttcaaac tttagaggtg atggaggtac tggccctcga gatggatggc tatatgcatt
     2641 gacattaata ccatcagtta tgtttgcact tggagtagtt agagttatag accacttaga
     2701 tggaatgaag gcagctcaaa agcttcttag tccactatta aagccattat tagggttacc
     2761 tggatttgca ggactaactt tgatagctag tttacagtct acagatgcag ctgcatctat
     2821 gactaaagaa cttaaagatg atgggtatat agatgaaaaa caaaaagctg tattttgtgc
     2881 atttcaattt tcaggtgcgt ctgcaattac gaatttcttt gcttctggag cagcactatt
     2941 tccatttata ggagatgtac caatatttat accacttgct ttaatattaa ttatgaagtt
     3001 tgttggggcg aacttactta gattatattt aaataaattt gacaaggggg aagcttaaca
     3061 tgagtgaggt agcaaaaaaa gaagtaaaag ttaaaaaagg aaatgtgata gatgcgttta
     3121 ttggaggagc tagaaatgga ttccaaataa gtacaaattc aatggctcca aatgtcatct
     3181 tcggttttgc tataatttct gtgttcaatt taacaggtct tttggatgta atagggacaa
     3241 tatttacacc tgtaatgagt atctttggac ttccaggtgt tgcagcaaca gctataatga
     3301 caatatttat atcaatggga ggagcatctg gagtaatcgc aggtttattt acagcaggtc
     3361 aaataagtca agcacatgtt gcaattctat tgccaatgtt attgctttca ggagcgcaga
     3421 tacaatttat ggggagactt ttgggaacag ctgagttaaa aactaaatat tatccacata
     3481 tgtttgttat agcaacttta aatggttgta ttgcaatgct tataatgaat tatttcgtag
     3541 tataggtaat tataataaaa atttaatata aatggaaagt ttaaatttta aggaggagct
     3601 agattatggg aactaacaaa gattatattg agtatgtaca taaagtgtat gattatctac
     3661 acactatacc agaaaaagga tttgaagaat ataagacatc agagtttgtt ttaaaagagc
     3721 ttgaaaaaat ggggtttgaa aatattaata aaggagttgc aggaactggt attatagtaa
     3781 ctatagattc taaaaaagaa ggaccagtat ttgctataag agcagatatg gatgcattgg
     3841 aatttatcat agatggagaa aagaaaatga tacatgcttg tggtcatgat ggtcatactt
     3901 ctatgctttt aacagctgca aagttaataa aagagagaaa tttagtaaaa agaggtaagt
     3961 taaaaatact ttttcaacca ggtgaagaaa aattatatgg agcattaaga gtaattgatt
     4021 ctggtttgtt ggatgatgta gaagaaatgg tagggatgca tttaagacct ggacaagatt
     4081 gtaggttggg aaaggcgata cctgcaatgt accattgtgc tagctgtata atgcaattta
     4141 cgattaaagg tatcacttgt catggttcta aaccacattt aggagtaaat gcagtagatg
     4201 ctgtctgttt agcagtgaat gcaataaatg caataagagt aaatccagct ataccaaatt
     4261 ctataaaagt aacaaatata tcagttaagg gagaaactta taataatata ccagatgaag
     4321 catttatggc tttagatata agggcagaaa gtaatgatat aatagaagaa ttaaaagaaa
     4381 aagcaataca tgctgtaaaa actgctgtaa gttctataaa tgcagaagct gtactaattt
     4441 ccaaagatgg agtaccagca gcagaatttg atgaagaact tgtaaacttg gctgaagaaa
     4501 ctataaaaga tgtattggga gctgatgcat ctataggtat atttaaaaat tctggtggag
     4561 aagatttcca ttattataca caaaaactaa aatgtaaaac tacatatatt ggattaggag
     4621 ctgatgctac accaggattt cataatccaa atgtaacatt tgatacgaaa gctttagaat
     4681 atggtgtaga tatatggtgt agattggttg aaaagagatt gggataatat ccaaactaat
     4741 aattctacat ttaaaattat aattatagaa gttatagaaa aagattgctt atgttaatta
     4801 gaaaataatt aatatgagca atcttatttt gatataatga aactgatttt ggaatattga
     4861 aaaaatatga tggaaaagta attatatttt aaatctattg gacaaaaaag tgatattatt
     4921 ataatattaa gaattgtaaa ataataccaa agtgcatcta gtaatggact taacaattat
     4981 aaaaatttta ttcttaggag ggaaaaaatg agtaagtata attttgacag agtaatagat
     5041 agagtaggaa cagattgtgt aaaatgggat tttagaacta attgttcaac aaaagcacaa
     5101 aaagatggtt tgcctttttg gattgctgat atggattttg aatgtgcaga accaataata
     5161 gaagcattac gtaaaaaagt tgaacataaa atatttggat atagttcaaa tgatagtgat
     5221 aaatacttta atgcagtttg tgattggtat aaaaggagat ttaactggga aataaataga
     5281 aaagatatta tttttagtcc tggtgttgta cctgctgttg ctattttagt aagaatatta
     5341 actaattcta atgaaggtgt aatagtccaa aaaccagtgt attacccatt tgaagctaag
     5401 ataaagagca ataataggga cattgtaaac aatcctctaa tatatgagaa tggtacttat
     5461 aggatggatt atgatgattt agaagaaaaa gctaagtgta gcaacaataa agtactgata
     5521 ctttgtagtc ctcacaaccc tgttggaaga gtttggagag aagatgaatt aaagaaggtt
     5581 gtagaaatat gtaaaaaata tgacctatgg ataatagctg atgagataca ttctgattta
     5641 gttagaaaag gatttaaaca tactccttta cagtcattat gcccagagta caaggataaa
     5701 ataattacat gtatagctcc aagtaaaaca tttaatctgg caggaatgca gttgtctaat
     5761 ataatagtga ataatgatga actaaaaaag aaatatcaag aagaggttac agctgttgga
     5821 gttgcaactt caccaaatcc ctttgctatt gtagctacaa tagcagcata taatgaaagt
     5881 gaagactggc ttaatgaatt aaatgattac ttagataata atatacaatt tattgatgaa
     5941 tacttaaaag aaaacctacc aaaagtaaaa cttgtttatc cagaaggaac atatctagca
     6001 tggttagatt ttactgctta tggattaaat gaagtagaac ttgaggattt aatgtttaaa
     6061 aaagctaatg tattatttga tgaaggttat atatttggaa aagaaggaat aggatttgaa
     6121 agaataaatg tagcttgtcc acagtcttta ttaaaagaat gtatggacag attaaagact
     6181 atatttgaaa atttataaag tataggtaaa aatttataaa gtgtttaaaa ataaaaaaat
     6241 aataaacttg acaaatttta actaatcact tatcatacta ttatgaataa atagaaaaca
     6301 taagagagct aaattttatt gaattaaact cttaaagact ttgaaaggaa gagtagttat
     6361 agaatgtaat taaagcgagt tggtgatggt gtgagaccaa tatgaagtct ataatgaaga
     6421 acatcctgga gttgctaacc gaaattttaa aagagtaggc ttagtcggaa ccaaaccgtt
     6481 atcatattgg ggtgtatcga gtaatatctg tacattgtga gctgggtttg aatttatttc
     6541 aaactaatca gggtggtacc gcggaaaagc aacctttcgt cccttttata tagggatgaa
     6601 gggttttttt aatggaaaaa tatataaata tagggagtga atgatatgag cacaataata
     6661 ccaaaggatt ataaaagtag tttgaacgta attgatacac aaatagctat aaaaaaatta
     6721 aaagattttt ttgaaatgag actaagtaat gaattaaatc ttataagagt ttcttctccg
     6781 ttatttgtat tgccagaaac aggtgcaaat gataatttaa atggagaaaa ggctgtaagc
     6841 tttgatatac catttatgaa taaaaatgca gaaattgttc aatcacttgc aaaatggaaa
     6901 agattagccc ttaaaaaata tgggtttgaa gttggtagtg gtttatatac agatatgaat
     6961 gcaataagaa aaaatgaaga attagacaat atacattctc tttatgtaga ccaatgggat
     7021 tgggaattgg ttatagataa aagtagtaga aatgaaaaga ctctaaaaga tgttgtaaaa
     7081 agattgtatg gagtatttaa agatactgaa atttttgtat gtagtatgta tgaaggtata
     7141 aaagaaatat taccagaaga aataactttt ataacatcac aagagttaga agatatgtat
     7201 ccagaattat caccgaagga aagagaagat aaaattgtaa aagagaagaa agcagtattt
     7261 ataactcaaa taggaaaaac attaatatca ggagaaaaac atgatggaag aagccctgat
     7321 tatgatgatt gggaattgaa tggggatata ttattttaca atcctgtttt agattcagca
     7381 ttagaattat cttcaatggg aattagagtt gatgaagaat ctcttgatag acaattgaaa
     7441 cttgctggat gtgaagagag aaaagagttt gactatcata agatgctttt aaatggcgaa
     7501 cttccgtata caattggtgg aggaattggt cagtcaagaa tatgtatgta tttcttacaa
     7561 aaagctcata taggagaagt acaagttgga gtatggccac aggatatgat tcaaaattgt
     7621 agtagtgcag gaattgaatt attataattt aaatataaac taattgataa aatttatatc
     7681 taatatgttc atcaaattaa ttatatcggt tataatgtag aataacctat aattataaga
     7741 ggtgataaat atggagtttt taaaaataat cattaatata gttttagata ttttaaaaaa
     7801 aattttggtc agatttaaaa atgctaaatt tggtttggtt tttgtatttg atttacttaa
     7861 actacctgat tttatgacag ataaaagaat caatatagtt gataaaataa aggtaatttc
     7921 tgtattaatt tttactatat cctactttgt atctggggtt gatataattc cagagatgat
     7981 tgcaggggca tttggtttta tagatgatgc tatagtttta atttggagta taggtatagt
     8041 gaatgaagaa attaataagt atagagttat tataaaaaaa gataaacatt caaatataat
     8101 tgaaaatgta gaattttcta taaaagatga agaagaataa caatttatat atgttattct
     8161 tttttttatt aacaaaaaat ggttaaattg ttgacggaga ggtaaaactc tattatcata
     8221 ataagtggat atccaatatt taactaagta ttcaatgtgc aagaatgctt aatttaaaaa
     8281 agttatggag ggtaaatatt atgaagaaaa aaataggttt agtgcccaaa ctaatcatcg
     8341 gtatcatagt cggtatttta atcggaagtt atgcaccaga aataatagta caaatattag
     8401 taacagttag taccttattc tcagcattcc taaaatttgt tataccattt ataattatag
     8461 gttttgtaac agcaggtata gctgatttag caacaggtgc aggaaagtta ttggggataa
     8521 ctacgggaat agcatatggt tctaccttaa tagcaggatt attatcattt gcagtatcta
     8581 cattaatctt cccaaatttt attgatgcaa gtgttgcaag tcaaattgga gacccagagg
     8641 caggaatgtt agctccaata ttcacaattc cattagagcc tatggtagat gtaacagctg
     8701 caatcgtatt tgcatttgta atgggacttg gaatatcagc tttaagaaat catggaaaag
     8761 gagagacttt atttaatttc ttccaagaat ttcaaggaat cgtaacgaaa acgttgtcaa
     8821 cagtgattat acctttatta cctttataca tagcaggaac ttttgcaaat ataactatag
     8881 caggggaagt atggacaata ttaggggtat tctggaaagt atacttagta gtaatacctt
     8941 tacattttgt atatatggca tgtcaattca cagcagcagg tgtattttca ggaaaaaatc
     9001 cagtaagaat gttaaaaaat caagtacctg gatatttaac agctatagga actcaatctt
     9061 cagcagcaac tataccagtt aatgttggtt gtgcagaaaa aaatggagta agtaagcaaa
     9121 taagagaatt tgttgtacct ctttgtgcaa caatacattt atcaggtagt atgatatcaa
     9181 taacaggatt tacaactgca gttttaatga tgaatggtat gccacatgga ataggtgtat
     9241 tattccctta tattgcaatg ttaggtatag caatggttgc agcaccagga gcaccaggtg
     9301 gggctataat gagtgcactt cctttcctac caatggttgg aataccatca gatggtgggc
     9361 ttgcaagttt aatgatagct ttatacttaa ctcaagacag ttttggtaca gcatgtaatg
     9421 tatcaggaga taatgcaata gcagtagtag tagataaaat aaatgatgga atgagtaaaa
     9481 aaggaaacaa agaagagcaa agtgcttagt tttttaataa tcaaaaatga gttatctcaa
     9541 atagagatag ctcatttttt atggtataaa aaaattataa aataaaggga tttttttatt
     9601 taaaaattgc tttaagagat gatattaata atttattttc attaggtttt aatatacata
     9661 ctctaaagaa atactcatct agaccttcaa atgaacaaca atctcttatt attatttttt
     9721 gtggaagcag ttgttctcga agcgatttag cagttaattc ctttgttttg attttacaca
     9781 atatgaaatt acctttagta ttgtatatat ctaagctttt tatattctta agctctttaa
     9841 gcaggtaatc tctttcagtg ttcattaagg aaatagtatt tgatataaaa tctaaatcac
     9901 taaacatgat ttcccccatt ttagaagcta ttatgtttat attccataaa tctagatttt
     9961 tatttatttc atttttgaca ttagtgttag atataagacc atatccaagc cttattccag
    10021 gcgttgaaaa aaattttgat gtacctctta taacgaataa attagaataa tcatcaacta
    10081 attgagtaca agagtatgta tctgtatcag taaactctac ataagtctca tcaatcatta
    10141 aaaatgagtc tgtatttttt aaaatttctc tcacttctac tttagtaaat gcaaatccag
    10201 ttggattact aggattacag attactacca aatcataatc tttagcattg atagttttta
    10261 taagattttc taaatttata tgaaaattat cttcttcttt tgcaaagtat ttttctatag
    10321 aacaattaat tttagataac tctttttcat actctgaata ggctggagag agtaaaagag
    10381 cctgtttagg attaattgta tgtataaatg atgaaattag ttctgtagct ccactgccaa
    10441 gtactatatt atctatagaa catttgcagt aattggatat agaagttttt aagtctatgt
    10501 aatcagggtc aggatacata gataccatat ctatattgtt tacaatgtat tgtttggcta
    10561 aacttgatgt accaaaagga tttatatttg agctaaaatc cataaattct tcctttgaaa
    10621 agccatactt actagataaa ctatataaat tagctccatg atttgtgctc ataaaagtac
    10681 ctccaaaaat aaaattatat aatcgataaa aataactgat taatatatgt acatatagat
    10741 tataatacaa aggaatcgtt attccaaatt tttaatggaa aaagcaaaaa agtatggata
    10801 agctgttaat ttaggtctaa aaatgatgaa aaagatttta attgatattt tggtatgcta
    10861 taatatataa gttaatagaa ggaaagcggg aggatacaaa atgtacaaag ttacaggaaa
    10921 aatgttgaca gaagaacaaa taaaagaaaa agtttatgaa ttaggaaaaa agatagaaga
    10981 agactttaaa ggcgaagatt tactagtggt aggtatactt aaaggagcta gtgtatttgt
    11041 ttcagattta ataagatgta tagatttgga tgtaaatata gattttatga gtgttacaag
    11101 ttatggaaac tcaacagagt cttctggaac agttaaaata ttaaaagatt tagatgtaga
    11161 tattgaagga aagaatgtgt tgattgtaga ggatataata gattctggtt taacattaag
    11221 taatctagtt gcagctttaa aaactagaaa tccaaaatct ttaaaactat gtactctact
    11281 tgacaaacct cagagaagaa aagctaatat acctgtagac tatgttggat ttgttataga
    11341 agacaaattt attgttggtt atggaataga ctatgctgaa aagtatagaa atttacctta
    11401 tataggtata gttgaggatg tagaatagat ttaaatgtga atcaaaagca ctaatttcta
    11461 agggagttag tgctttttag ttaattatat aaaattttag taaaggagac atctatatgg
    11521 atattcagtt aaatatatta ttatttttcc aaaatttaag aaatccaatt ttgaacttta
    11581 tatttcttgt ctttacaata agcacagaag caccgttatt aatattaatt acaacaatta
    11641 tatactggtg tataaataaa aaatgtggac agaaaatact gtttgctata attggaaatt
    11701 ttgttgtaaa tttaggcatc aaagagtttg taaaagctcc cagaccaata ggaataaaag
    11761 gtcttgaatc attgagagtt tctactgctg gtggttattc ttttccaagt gctcatactc
    11821 aaactgcaac aacattttgg gttagtatta tgactatatt taaaaagaga tatcttcatt
    11881 taataggtgc agttatggtt ttaggagtag gattgtctag gctgtattta gctgttcatt
    11941 ggccaataga tgtgatagtg ggttggatgt taggaatatt ttttacagtt atatttataa
    12001 aaatatttga ctatattgat gataataaaa attatatttt acttgtagtt ttacttatac
    12061 catttataat agtagctata tttctaaata gccctgaata ttttgaaaaa tttggtataa
    12121 tagtaggatt tgtatttgga tatatggttg aggatagatt tgttaaattt aatacagata
    12181 ataataacaa aaaaataaat tttagcaata aaaataaggg gagtaaaaat ataatttttt
    12241 caagaatatg taggtttttg gttgggatag taacaatagg aatgttatat attggcatta
    12301 agttgtttat ttcaatgtta atagtcactt taaatataag tgactctact atgagtataa
    12361 tttttatgaa ttttattaag aatacagtag ttgtttttta tggaatagca ggagctccag
    12421 cattatttaa acttttaaaa ttaaattgat tttaaggtat agagtcaata gtttaaatta
    12481 aaaaaattaa gggtagaaaa gagtgattat ttgaagaagg tagtaataat aggtggtgga
    12541 ccagcaggga tgatagccgc atcaactgca tgtgagaaag gctatgatgt aacattaatt
    12601 gagaaaaatc ataaattagg aaaaaaactt gcaataacag gaaaaggtag atgtaatatt
    12661 actaacgctt gtgaaataga ggaattaata gaaaatgtac ctacaaatgg aaaatttctt
    12721 tatagtgctt tttatacttt tacaaatgat gatgtaataa gtatgtttaa taatcttgga
    12781 gtaaagacta aaacagaaag aggaaaaagg gtttttccag agagtgataa agcctttgat
    12841 attgtaaatg ctcttgaaag acaacttaaa agtaagaaag taaatatatt attaaattca
    12901 aaagtagaaa aaataatatc taaaaataat aaaattgaga aagtaatttt aaacgataaa
    12961 aaagaaatta agtgtgattc agttgtagta gcaactggtg gattaagtta tccacttact
    13021 ggttctactg gtgatggata taaatttgca atttcacagg ggcacacaat tatagataca
    13081 aaaccatctt taattggtat agaagtacaa gaaagtttta caaaagattt agaaaaatta
    13141 tcattgagaa atgttgaaat aagggtgttt aatagcaaac aaaagaaagt ttatagtgat
    13201 tttggagaac ttgaatttac tagatttggt ttagatggac ctataatcaa gtctgcaagt
    13261 tgtagaatga aagatacaag gaaagaaaat tacactatat tattagattt aaaacctgca
    13321 ttagatgagg aaaaactgga taaaagagtt caaaaagatt ttcagaagta tacaaataaa
    13381 aaatttgaaa aagcattaga tgatttactt ccaaaaaaac taatacctat aataataaac
    13441 ttaagtgaaa ttaatgcaaa tacggttgtt caccaaattt ctagagagca gagaaaaaac
    13501 ttagtccatt tattaaaaaa tttaaagttt acagtaaaga gatacagacc tattgaagaa
    13561 gctataataa cttctggtgg tgtaaaagta aacgaaataa attcaagcac aatggaatca
    13621 aaattagtag aaggtttatt ttttgctgga gaagtaatag atatagatgc atatacaggt
    13681 ggattcaatt tgcaaatagc tttttctact ggatatttgg caggttttaa ttgctaaaaa
    13741 ttacctgaat taaaaatgtt taaaatcaca taatattatc agcaacacaa aataaattaa
    13801 acagaaggaa ggtaatcaac atggcaagta acaataacaa caacagaaca gtagtaccag
    13861 gagcaaaaga ggctttaaac caaatgaaat tagaaatagc taatgaactt ggtatgagta
    13921 attatcaaca agttgataaa ggaaacctta ctgcaagaga aaatggttat gtaggtggat
    13981 acatgactaa aaaattagtt gaaatggctg aacaacaaat ggctggaaaa agcaacagat
    14041 agttataaat aattatttat aactattggc tattagttta gctagagcta tctcaaatat
    14101 agaaagtaat ttctaaattg agatagcttt tttttattaa attattaaat agatatattt
    14161 ttatgtaata aattttatgt atttggataa aataatacaa atgaatatgt tacataagga
    14221 gtgatgtaaa atgagtagtg taaattttga ggatttaaaa aacaaattta ttaatagtga
    14281 cttagatgaa aaaataagaa tatatacaac aacagaaggt ttatcagtag aacaatttaa
    14341 agagctacta aaatattatc caatacaaca tttatctaaa ctagaaaaag cacttggata
    14401 gcagtattta gtccaaaatt aaaaaggc
//
LOCUS       DACTGW010000088        14242 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.088, whole genome shotgun sequence.
ACCESSION   DACTGW010000088 DACTGW010000000
VERSION     DACTGW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.088"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..581)
                     /locus_tag="I9573_002013"
     CDS             complement(<1..581)
                     /locus_tag="I9573_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4796633.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNALIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEI"
     gene            1052..1702
                     /locus_tag="I9573_002014"
     CDS             1052..1702
                     /locus_tag="I9573_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HAT4796634.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(1842..3218)
                     /locus_tag="I9573_002015"
     CDS             complement(1842..3218)
                     /locus_tag="I9573_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT4796635.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(3234..5147)
                     /locus_tag="I9573_002016"
     CDS             complement(3234..5147)
                     /locus_tag="I9573_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAT4796636.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            5286..5915
                     /locus_tag="I9573_002017"
     CDS             5286..5915
                     /locus_tag="I9573_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAT4796637.1"
                     /translation="MGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGFT
                     ASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANYA
                     GFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQE
                     VINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            6063..6593
                     /locus_tag="I9573_002018"
     CDS             6063..6593
                     /locus_tag="I9573_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase"
                     /protein_id="HAT4796638.1"
                     /translation="MEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDEEIV
                     CISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKPFQM
                     DREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEEGKK
                     VCLDCFNKYERFYEEY"
     gene            complement(6769..6939)
                     /locus_tag="I9573_002019"
     CDS             complement(6769..6939)
                     /locus_tag="I9573_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAT4796639.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            7224..8291
                     /locus_tag="I9573_002020"
     CDS             7224..8291
                     /locus_tag="I9573_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HAT4796640.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSIEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSSSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            8461..10362
                     /gene="cooS"
                     /locus_tag="I9573_002021"
     CDS             8461..10362
                     /gene="cooS"
                     /locus_tag="I9573_002021"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HAT4796641.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            10355..10828
                     /locus_tag="I9573_002022"
     CDS             10355..10828
                     /locus_tag="I9573_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAT4796642.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            10803..12029
                     /locus_tag="I9573_002023"
     CDS             10803..12029
                     /locus_tag="I9573_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAT4796643.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSV"
     gene            complement(12168..13355)
                     /locus_tag="I9573_002024"
     CDS             complement(12168..13355)
                     /locus_tag="I9573_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT4796644.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKSFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            13628..>14242
                     /locus_tag="I9573_002025"
     CDS             13628..>14242
                     /locus_tag="I9573_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT4796645.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSI"
BASE COUNT         5379 a         1775 c         2230 g         4858 t
ORIGIN      
        1 attatttctt ctgagtattt aactagatta gattgtattt cctttgaatc aatttgtaat
       61 aaatttatta tttctataaa tgtagaaata tctatatatc tgtgacctaa atcaaatata
      121 tctgagataa agctaggtac tttttctgga actcctggag ttatagctat ttttatattt
      181 ttatagttat tatcattcat atatggaatt ggtctaatat tagtcactcc aattttataa
      241 aataaactta tagtttctag agtagtttca tcagaatcat ttacgattag agcatttgta
      301 ccctgtggta aagatagtag attataaatt ttatcttccc taaaagttct tcttaccaca
      361 atcacttttt tcttatctag tatattattt ataattttgt ctaatctttc tactgacatt
      421 actaatatca catcatcatt tataatatca ttatcattta tttcatttat gaagtatcta
      481 ttaatattta tagaatctcc aaaaactaat ttcaaattat tttctaaaaa gtctattaaa
      541 tcttttctac tatcatttac cagagctact gattttttca tttatttctc cttatataat
      601 aaatagtttc caacaaaata gttgtataaa gcaatgtaat tttgataatt taaatttatc
      661 aaataaaaaa gaaagtgtct ataatgactt tataaaagtt gcattaagac actttctttc
      721 ttataattaa aaatcttgtc accaaatccc cctaattgtt gcttaaaact taaactgtat
      781 gtatattttt catttctatt cgattaaatt ataatataca cccaaaaaaa tttattttaa
      841 taaattctat tgataatata attttgtaaa tatagaatta tattaaaata aattatatat
      901 gaaaatatat atgtataagt ttattctgcg tgagtagaat gaatttgtca agtttaattt
      961 agttgtaatt gtatctttta ttaaaaataa tataaattaa aacatataaa taaaatattt
     1021 tatacaatat attgtaaagg aggtaacttt gatgaaagca gaaaaaacaa gtatatatcc
     1081 aattattaaa aataatttaa acaaaatatc aattgttgaa tataaaaagg gtcaaagatt
     1141 tgtagcatca gataaagact tacaagaagt gttctttata atagagggag ttgcattagt
     1201 ggagtgtact actagaggag gaaataaatt tcttgttgac attgtacctg aaaatgaatt
     1261 tataggcaaa atcagttaca tatatgaaca taatttgaaa tgtgatatat ttgcaaagac
     1321 caatataaaa ttatttcgat ttgaaaaaaa tgtatttgaa gagttttatc tcaaaccaga
     1381 ttttattgca ctatttcaca gaaaatgtac aagaagaata tatgaattat ataaaaccag
     1441 aatggtaaga gaactatttt cttgcacaga agttattgcc tattgtatat caaataaaca
     1501 agaaaataat gtctgcaata taaaagctat tcgtaatttc agtgaaatct attcaattag
     1561 tagaaaaagt agataccatt ctctgctaaa gttaactgaa aaggagatag taaaaaaaat
     1621 gggtaataca tatgaaattt taaactatga tgaattacat aaattggcat ttgaagtaaa
     1681 agagtttttg gaagatgagt aaaaattgtt acttaatcaa aataaagtat aataatttct
     1741 gtaaaaaata gaaatagtct cactgaaaat caaaaatact ttagttattt tcaatgagac
     1801 tgtttagaaa tattgtatac ttataatgta tagtataatt attaatcaat ttcctttact
     1861 atattaatag cccatttttt agataatgct ctaggaacac caaatataaa cttaaccact
     1921 tcttcactat aagtgagtaa aacgagtaca aatataggtg cacctttaaa tgcagctata
     1981 aaagttagag gaatagaagc taaccacata catcctaatt ctagaaatag agcatattta
     2041 gtatctcctc cacttcttaa aataccaatt ataaccaatg tattaaaaga tttaagagcc
     2101 attagtattc ccatgataaa gaatatctta gttatatcag aagctaagct atcagaaaca
     2161 ctaaacattt ttaataataa tggtatattt aaaattaaaa taattccaag tattaaacca
     2221 gctgaaaata caagtataga aaacttttta gcatattcaa tggctctttt aatatggtca
     2281 gctccaagtt catttccaag cataatagat gcaccagatg caatacatac agctgtcatt
     2341 atgaagaaat tacctgtact ggttgctatc tggctagctg ctatagcaga agtaccagct
     2401 cttgcatatg caacagaata caggacagtt ccaattgccc atacagagtc atttacaaat
     2461 ataggaaagc tttttgatga aaaagatttt ataaaaattt tatcaatagc ttttagattt
     2521 tttagaccaa actttaatat atagtctttt ttataaaggt aaacataagt aatcattaaa
     2581 attaattcac atattctagc aataacagta gctagagcag caccttttac acccaatgcg
     2641 ggaagaccga agttaccaaa tataaatcca taatttaaaa taacatttgt tacaagtgca
     2701 aatgcactac aaatcatacc taacttagga tttctaacac ctcttgaacc agtactaaat
     2761 acagtactta cagcaattaa tggataacta aatactgcaa tactaaagta atctatacaa
     2821 agttttacca cctcagaatc atacgaaaat atgtgtataa ttggctttgg tgttagtaaa
     2881 gcaggaataa caaatacaaa acttaaaaca attgccaata cacaagtaag acctgtgact
     2941 tttcttatat tactcacatc cttctttcca taaaactgtg aaataaaaac tccagcacct
     3001 cctgtgatac cagataagga catattaaaa agaaagaaaa attggtttgc aactccaact
     3061 gaagctacag atgtctcccc caaactactt atcataattg tatctattac atttacagaa
     3121 gtagaaatta agttctgtat gataataggt atacataaag atagaagtat tttataaaat
     3181 cttttatttt cgtcttttaa agaaaatagt tcttgcaaat aatcctctcc tttttacata
     3241 aattcttccc actcatcata tagagaggca aggttatttt ctaaattaat tttttcttgg
     3301 ctaacttctt tagctttatc tggattagag tatacttctt cttgacataa aagtatatct
     3361 aatttttcaa tttcaatctc taacttttca atttcttttt ctatattttg tctttttatt
     3421 ctatttttct tttctatttc tctttgttct ctctcttttc ttttttcatc ttttatttga
     3481 gttctagttt tttcttcttt ttcttctata atactcatct cttggatttg ccttttttta
     3541 tctatataat aatcataatt tcccagatat tcagtaattc cattttcatc taaaactaaa
     3601 attttatcaa caacagtatt tagaaagtat ctatcatgtg atattgtaaa tatagtccca
     3661 tcataattag ttaatgcctc ttcaaggact tccttagaat caatatctag gtgattagta
     3721 ggctcatcta ataacaataa gtttgcattt gatagtataa gtttaagtat ggcaactcta
     3781 gctctttctc caccactcaa tgtagaaatc ttcttaaaaa cttcttcatc tacaaataaa
     3841 aatgccccta acatatttct caaagtagtt tgagtaagat gagtattatt ttcccaaatc
     3901 tcatcaatta ttgtgttatc taaattaaga gttttttgtt cctgatggaa gtaggaaaca
     3961 tgtacattag ttccaaattt aatattgcca ctaagaggtt gtagttcatt agttattatt
     4021 ttaaataatg tagatttgcc aacaccattt gcacctataa gtgcaacttt ttcccctcta
     4081 tatatatcta aatccaagtc tttaaaaagt attctttctc cataacccat agatacatct
     4141 ctaatttgaa gaacatcatt accactctgg acagaaggcg taaattgtat acgagctttt
     4201 ttcctataag cttctggttt atctaaaacg tctaccttgt ctaatgcttt ttctttactt
     4261 cttgcacgct ttaggtgttt ttctctacca taagctttta gtctttcaat tgattcttct
     4321 tgctttttta tttctttttg ttggtcttca aatttcttaa gctctagttc tttttctatt
     4381 ttagataact caacaaattt agagtagtta ccattatata ctttaagttt tttgttgtga
     4441 acttcaaaaa ttctatttac ggattggtct aagaaatatc tatcatgaga tattaacatg
     4501 acagttcctt tatattgttt taaaaatact tctaaccatt ctattgcttc agagtctaag
     4561 tggttagtag gttcatcaag taacaataga gttggtttct taagaagtaa ttttcctaaa
     4621 agaactctag ttttttcacc accagaaagt atgcttattg gcttatccat atcattgtca
     4681 ctaaatccta atccttttag aactcctttg gcttcagatt tatatccata tccatttaaa
     4741 tctgaaaata attctagttt atgtgaatat tcatccatga ttttttctat aattggagag
     4801 tttgtttttg tactttcttc agatatttta acctccaatt ctcttaaata ggattccatt
     4861 tctatcaaat ttttaaaaac ttctagcact tcttccaaaa tagtattatc cgaataaaaa
     4921 ttggtatttt gttctaaata tccgatttca caatctttag aagtataaat atctccacta
     4981 tcatatccat aaattcctga aattatttta aataaagtag tttttccagt accattaaca
     5041 ccgattatac caattttatc gccttcattt actgtaaaac taatattttc taatatagaa
     5101 tctataccaa aacttttatt taagttatta catgacaaaa caatcataaa tttctacctc
     5161 cttgaactat ctaaaattat aatgttttga tataatataa tcaaggttat atgtattagt
     5221 tttgaaaaag aaatatttta ttgtgaatca aacaaaaaag aggacacttt taggaggtga
     5281 agatgttggg aaataaaaat atatcaatgg cagttataag aaggctccca aaatatcata
     5341 gatatcttgg agacttatta gatagggata tacaaagaat atcttctaaa gaattgagtg
     5401 atataatagg gtttaccgct tctcaaataa gacaagattt aaacaacttt ggtggatttg
     5461 gacaacaagg atatggttat aatgtagaag ctcttcatac tgagataggt aaaattcttg
     5521 ggttggatcg accatacaac gcagttcttg taggagcagg taacttagga caagctatag
     5581 ccaattatgc aggatttaga aaagctggat tcgagataaa agctttattt gatgcaaatc
     5641 ctagaatgat aggtttaaag ataagagagt ttgaagtatt agattcagat actttagaag
     5701 actttataaa aaacaataat atagatattg ctgtattatg tatacctaaa aatggagcac
     5761 aagaagttat taatagagtt gtaaaagctg gaatcaaagg tgtatggaat tttgcacctt
     5821 tagatttaga agttccgaaa ggtgttatag ttgaaaatgt aaacttaaca gaaagtttat
     5881 ttaccttatc gtatttaatg aaagaaggaa agtagacaac ttaaacatat taataatgag
     5941 cagttgtgaa gagtattgag aaagtcagaa gatttaatat taaatcctct ggctttttga
     6001 aatattagga taaaaattta aataatgtat ggaaataaac aatattaagg agagtggttt
     6061 ttgtggagga attatggaaa aaggcaattg aatttcatgg tcatgaatgt ccagggttag
     6121 ctataggatt taaagcagca cttgcagcaa gaaaatattt aggatgtaaa cagtcaggag
     6181 atgaggaaat tgtatgtatt tctgaaaatg atgcttgtgg aatagatggt atacaagtcg
     6241 tgctaagttg cacaatgggt aaaggaaatt tactacttag gatgactggg aaatcagtat
     6301 atcatttctt taatagggaa aatggaaaat ctataagaat atatgtaaag tcaaagccat
     6361 ttcaaatgga tagagaagga tacaaaaagt acttattaga aagtgatttt gaagaaatat
     6421 ttgagattac agatactaag ttaagacttc ctgaaaaagc aagaatattt aaatctctta
     6481 aatgtgaagt gtgtggtgaa tttgcatctg agcaatatat aaggattgaa gaaggaaaga
     6541 aagtatgtct tgattgcttt aataaatatg aaagatttta tgaggagtat taaaattata
     6601 aattagatta aagttaaaag tattagatat gtactaacag tattttaaga tgaattaggt
     6661 agcaacataa taaaatatat aaataatatt acttgaacca tacataatta aaaagaggct
     6721 gattagtatt tagaaaataa aatcagtctc tttatgaata acgaattatt attctggttg
     6781 aggtgcatct acaggacaag ctcctgcaca agaaccacag tctatacaag aaccagcatc
     6841 tataacatat gcgtcgtctc cagctgatat acagctaaca gggcattcag cctcacaagc
     6901 accacagctt atacaagcgt cagtaatttt atatgccatt ttgaaatcct cccttttata
     6961 tgtattgtaa ttcaataaca ttataacaaa tattattgaa tataacaatt aaattaattc
     7021 tacaacaaaa actttattat gatattcttg ttttaaagtt ttacaataaa gtttaatctg
     7081 cttatatttt tatttatttt aaagaagttt attttaaaaa taaaattaaa tttaaaaagt
     7141 tatgtattga ttatatttaa aaatgttata ctctaaaaaa gtatatttta ttttaaaatt
     7201 tattaataca aaggagattt actatgaaaa ataaattagt ttacatatgt attatgagtc
     7261 tattgctatt tgtagtagga tgttctaata atccaaataa ggaaagtacc attaaaaaag
     7321 actctaagaa taaccaaagc caagttaaag aagaaaaaag agaacctgtg aaaaaagata
     7381 taactataag tttcgctggt gatgtaacta tggggaacta taaaggttca tcatattatg
     7441 gcagttttga ccatgaattt aaaagacaag gagaagatta caattatttt cttaaaaatg
     7501 taaaacctgt atttgaaaaa gatgacttaa cagtggttaa tttagaaggt ccacttacta
     7561 cagcgtcaaa tgcaaaagaa aaaaaatttg catttaaagg aaagcctgga tatgtaaata
     7621 ttttaaaatc tggaagtata gaggcagtaa gtattgcaaa taatcattca gaagattatt
     7681 ttgaagaagg aatgaaagat acacagttta tattggatga gaataaaata aattattttg
     7741 gattagggaa agatgctgtt gtagatgtca aaggaattaa agtgggtcta ttagggtata
     7801 atggattatc aactgaatat aatgaaaaaa atttaaaaca aatggaagat gatattaagt
     7861 ccttaaaaaa gaaatcggat gttgttgtag tgtactttca ttgggggata gaattagatt
     7921 attatccaga taaaaaacaa aaagattttg ctcattatgc tatagacaag ggagcagatt
     7981 tagttatggg gtctcaccca catgttattc aaggaataga aaagtataaa aataagtata
     8041 tagcttatag tctagggaat ttttgctttg gaggaaataa gaatccttct gatacagata
     8101 gttatatata tcaacaaacc tttacttttt cagataataa attaacttca attaaagagc
     8161 caaatataat accaacctca ataacatcta gtagctccag aaataattat caaccaaaag
     8221 tattggatgg aagtgagaag gatagagttt taaataaact tgaaaaaata agcaaagatt
     8281 taaataaata aaatgtaaaa aaaaataaat atatatattt attttaataa tttatatttt
     8341 gtttagggta tggtaaatcg tggtattaaa taagtagatt ttaatagtta aattatagtc
     8401 aattataata aagctattta atctactttt ttaatataaa aatataaggg ggaatttgta
     8461 atggcttgta acacttgtaa aatgtgcgaa agtgcagaca agaaattaga aagttttgta
     8521 gcttcaaagg atgttgaaac agcatttcat agaacagaag atcagaaagt taagtgtggt
     8581 tttggacttc agggggtttg ttgtagactc tgttcaaatg gaccatgtag agtaactcct
     8641 aaatcaccaa gaggtatatg tggagcagat gcagacacta tagtagcaag aaacttttta
     8701 agagcagtag catcaggagc agcttgttat ttacatgtgg tagaaaatac agctaagaat
     8761 ttaaaaaatg ttggaataac taaaggtgtt gttaaaggag aaaaaacatt aaatcaatta
     8821 gcagagatgt ttggaataga aagtgatgaa aagtatgata aatgtataaa ggttgcagac
     8881 aaagttatca acgatttgta taaatcaaga gatgataaaa tggaattagt agaaaaaata
     8941 gcttatgcac ctagagtgaa aaagtggaaa gagttaggta taatgcctgg tggtgcaaag
     9001 tcagaagttt ttgatgcaat agtaaaatca tcaacaaatt taaatagtga tccagtagac
     9061 atgctagtta attgtttgaa cttaggtata tcaacaggat tatatggtct tacactaact
     9121 aatcttttaa atgatgttat gttgggagaa ccagttataa gaatggctcc tgtaggattt
     9181 aatgtaatag acccagacta cataaatata atgataacag gccatcaaca ttcaacattt
     9241 gcaaatttcc aagatagatt aaaagatgaa gatgttataa aattagctca atctgttggt
     9301 gcaaaaggct ttaagctagt aggatgtaca tgtgttggtc aagatttaca attaagagga
     9361 gaacattatc aagaagtatt tgctggacat gcaggtaaca attttacaag tgaggctgtt
     9421 ttatcaactg gagcaataga tatagttctt tcagaattta actgtactat accagggctt
     9481 gaaccaatag cagataaata taaagtaaaa atggtttgtt tagatgatgt tgctaaaaaa
     9541 gcgaatgcag aatacatagg tttagacaga agtaaattag atgaacttag taacacttta
     9601 atagaaaaag cattggagtc ttataaggag agaagaggaa gtattgagat agatatacca
     9661 aaagaccatg gatttgaaca atctttaact ggtgtaagtg aaaagaacct gaaagcgttt
     9721 ttgggagatt catggaaacc tcttattaat ttaattgcag aaggaaaaat taaaggtgtt
     9781 gcagctgttg taggatgttc aaatatgaca gcaggaggac atgatgtaaa tactgttgaa
     9841 cttactaaag aattgattaa aaaagatata atagtactat ctgcaggatg ttctactgga
     9901 ggattagaaa atgttggtct tatgtcacct ggagcagaag aattagcagg agaaaactta
     9961 aaagaagtat gtaagacttt aggaatacca ccagtattaa actttggacc atgtctagct
    10021 ataggaagac tagaaatagt agcaacagaa ttagcagctg aacttggaat tgacctacca
    10081 caattaccat tagtgctttc agctccacaa tggttggaag aacaagcatt agctgatgga
    10141 gcgtttggtc ttgcccttgg cttacctctt catttagctt tacctccatt tataacagga
    10201 ggaaaattgg tgacagaagt attaacagaa aaattaaaag acttaacagg aggacatgtt
    10261 atagtgaatc cagaccctaa atcttcagct aatcaattag aagaaataat aatagataga
    10321 agaaaaaatt tagggttaaa ggatgtggaa tgcaatgcat agaattttta taaataaaga
    10381 tttatgtaca ggatgtaaga gctgtgtact agcatgtatg ctaaaacaca ataaagatta
    10441 tgacatgtac actcttgatt tagaaaatat agataatgat agtagagggc atatagaatt
    10501 agatagtaaa cataataatc cagttccaat tctttgtaga cactgtgatg aacctgaatg
    10561 tgtattagca tgtatgagtg gtgcaatgca taaagatagt gaaagtggaa tcgtttcata
    10621 tgatgaagag aaatgtggtt catgttatat gtgtgtaatg tcttgtccat atggattatt
    10681 aaagccagat gatagaagca agcaaaacat acttaaatgt gatttatgta aagatgagga
    10741 gtatcctaga tgtgtagcta attgcccaag tggagcaatt gaattacaga aggaggaaaa
    10801 tgatgaatta tgtagtgtta ggggctagtg cagctggtat aaatgcagta aaaacattaa
    10861 gagaattaga taaagattca aatatagtag taatttctaa agatgaaaac gtatattcaa
    10921 gatgtatgtt gcatcacgtt atatctgaac acagaacttt aaaacaaata aattttgttg
    10981 atgaagattt tatggagcaa aataatgtaa aatggattgc aggtaagact gttaaaggta
    11041 tagacataaa taaaaaggta gttcaaacag aagacattac tgtaaattat gataaacttt
    11101 taatagctac aggtgcatct tctgctattc ctcctataaa aaatttaaga gatggaaatt
    11161 ttgtatattc tgtgagaaac atagatgata tttataaaat aaaagaaaaa gcacaaaatt
    11221 ctaaaaatgt agttataata ggtgctggtt tagttggtat agatgcatta gtgggattat
    11281 ttaaatatga gcagttaaat atttctgtag cttttatgga aaagtacatt ttagatagac
    11341 aattggatga atatacagca tcagtgtatg aaaataagtt caaagaaaaa ggtgtaaaat
    11401 tttacccaag tgcatcaata caagaaattg tattggataa cagtaaaaat gttacaggtg
    11461 ttgcattttc aaatggtgaa gttttagatg cagacatggt aattgtagct acaggggtta
    11521 aaccaaatgc tgacttttta gatgggacag gaatagaata tgatagaggt ataataatag
    11581 atgatatgtg ccaaacaaca caaaaagaca tatatgcagc aggagatgtt gtaggtaaaa
    11641 atgctatttg gcctctggca gtaaagcaag gaattgtagc tgcctataat atggttggaa
    11701 aagataagaa gatagaagat gaatttgctt ttaaaaatag tatgaatttc atggatatac
    11761 caactatttc tataggaatg aatacacctg tagatgatag ctataaagta ttaaccagac
    11821 atggaattaa tgattataag aagtttgttt ttaaagataa tgtaatctac ggagcagtta
    11881 tacaaggaga tatttcatat gtaggtgtat taacttattt aataaaaaat aaagttgaaa
    11941 tatatgattt agaaaatagg atatttgatg tatgttatgc agactttttt aatataaaag
    12001 aaaatggaga attttgttat agtgtatagt atatacgtgg agtaaggttt ataaatacag
    12061 taaatcaata aatagagtta ttctaaaata agaaaaatga caaatcttat ttagaaatag
    12121 ctctattttt tgtaattaaa tttaaattat attcaatttg taaaatatta ttttgaacaa
    12181 ttatacatat actctctagt tagaggtaaa tcatttttta tacctttagt gaaaagtatt
    12241 tgatgtatat ctataatacc agtgttgaaa gcagcagcac aagaacaaag gtataaatcc
    12301 cacattctta taaacttatc atcaaacatt tctttaatct ttggaagatt ctcagagaaa
    12361 cttttttgcc agtatagtaa ggtttttaca taatgtaatc ttaaactttc tacgtctata
    12421 gtatgatatt tgtaatcact acaaatatgt atcatttctc ttaggctagg aataactcct
    12481 cctgggaaaa tatatttttt tatccaaggg tctccataag attcctgcaa tccacttata
    12541 tagtgaagta aaaacaatcc accatctttt aaaacagcat ttacgttttt aatgaaaagt
    12601 tcatagtttt ctctaccaac atgttcaagc attccaacgc ttacaactct atcaaacata
    12661 aggtctgatt tttttaattc tctatagtcc ataagtctta cctcaagata gtcttgaagg
    12721 ttctcttctt taattcttgc gttaaattcc ttaaattgct cttcgctaag tgtaattcca
    12781 acgccgtgaa ttttatattt ttttgcagct tctattagta aaaatcccca accacaacca
    12841 atatcgagaa ggctcatacc ttcctctaaa tttagtttag ttaatatgtg gtgaacttta
    12901 ttgacttgag catcataaag agtatcatta tcatttctaa agtatgcaca agaataacta
    12961 agtgtcttat ctaaccataa gctataaaaa tcgttgccaa tgtcatagtg ggcatgtatt
    13021 tctttcttct gatgtctcat tgaatttgat gagtgcaata gatgttttac agctgaatta
    13081 tcaatattga atttgtctat ttgttttaaa agtagattta aagttaagaa taaatctccc
    13141 tcaacttcaa tgtctctatt tatgtaggct tctccaagtg ctaaagatgt actttttaat
    13201 agttcagact tgctaatatc attatttact ataactttga actgagggga gccctctcca
    13261 attagaaatt cttcctcatc tttaagccta actagaaatg gtgtttcacc aaatttctct
    13321 aaatagtctt ttaaaaaatt ttttgtaata ttcatattac cagctccttc taatgtaaat
    13381 ccagtaagtt tccctgctta aaatatatta tagtcctttt tttaaaaaag taaaattttt
    13441 tcaataaatt ttttatttaa aggaaattta ttttttataa agaaatttca tgttgtagta
    13501 tattttttgc ttataaattt tggtactgga tataatatta aactgttaat gatataataa
    13561 atttaatcta taaagtaaat aaaattttgt aataaatagt taaagctatt ttataaggag
    13621 atatatgatg gaagtatatg ctttggttgg gtcaagtggc acagggaaga gctttaaggc
    13681 tttagagttc gcatatgaaa atgatataga atatataatc gatgatggga ttttaatata
    13741 caaaaacaag gttttagctg ggatatcggc aaaacaagca aatactacta tagaagcagt
    13801 taaaagggct attttttata atctggagca tagacaagaa gttagagaag caattaaaaa
    13861 agaaaatata agaaaaattc ttattattgg gacatcaaaa aaaatggtta atcaaattgt
    13921 agagagattg tcaattggaa gggtctgtaa gtttataaat atacaagata taagtactaa
    13981 atatgaaata gaaatagcaa aacaagctag aaaagaagga aatcatgtaa ttcctgttcc
    14041 agccgttgag ataaaaagta tggcaagtgg tctcagtata aattctttga agagactttt
    14101 tagaaaagga aataatagga atatggtagt tttagaaaaa acaattataa gaccaacttt
    14161 tagttatata ggaaagtttt atattagtgc tgatgtaatt aaacagatta ttgaatatga
    14221 agtatacaac tttggaagca tt
//
LOCUS       DACTGW010000089        14055 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.089, whole genome shotgun sequence.
ACCESSION   DACTGW010000089 DACTGW010000000
VERSION     DACTGW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14055
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.089"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            98..889
                     /locus_tag="I9573_002026"
     CDS             98..889
                     /locus_tag="I9573_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HAT4796646.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            918..2138
                     /locus_tag="I9573_002027"
     CDS             918..2138
                     /locus_tag="I9573_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAT4796647.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2158..2853
                     /gene="pxpB"
                     /locus_tag="I9573_002028"
     CDS             2158..2853
                     /gene="pxpB"
                     /locus_tag="I9573_002028"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAT4796648.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            2883..3869
                     /locus_tag="I9573_002029"
     CDS             2883..3869
                     /locus_tag="I9573_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HAT4796649.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRSKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLNSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4088..4654)
                     /locus_tag="I9573_002030"
     CDS             complement(4088..4654)
                     /locus_tag="I9573_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4796650.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5006..6073
                     /locus_tag="I9573_002031"
     CDS             5006..6073
                     /locus_tag="I9573_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HAT4796651.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEESKDISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6092..7252
                     /locus_tag="I9573_002032"
     CDS             6092..7252
                     /locus_tag="I9573_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HAT4796652.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEETELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            7265..9361
                     /locus_tag="I9573_002033"
     CDS             7265..9361
                     /locus_tag="I9573_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HAT4796653.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGFNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTKEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(9520..9864)
                     /locus_tag="I9573_002034"
     CDS             complement(9520..9864)
                     /locus_tag="I9573_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAT4796654.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            10156..11046
                     /locus_tag="I9573_002035"
     CDS             10156..11046
                     /locus_tag="I9573_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAT4796655.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            11264..12550
                     /locus_tag="I9573_002036"
     CDS             11264..12550
                     /locus_tag="I9573_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAT4796656.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDICEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKKFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(12639..13082)
                     /locus_tag="I9573_002037"
     CDS             complement(12639..13082)
                     /locus_tag="I9573_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796657.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(13106..13900)
                     /locus_tag="I9573_002038"
     CDS             complement(13106..13900)
                     /locus_tag="I9573_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid amidase"
                     /protein_id="HAT4796658.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
BASE COUNT         5375 a         1619 c         2468 g         4593 t
ORIGIN      
        1 aagtgataaa atatttcatg caatatataa aataaacaaa atttaaaaat taaaactact
       61 tattctatca aaatgttagc aaggaggggg tcataaattg tataaagtag atttaaatag
      121 tgatttagga gaaagttttg gaacgtataa gattggttta gatgaagaag ttttaaaata
      181 tatttcatca gctaatatag cttgtgggtt tcatgcaggt gaccctagtc atatggaaaa
      241 aacagtacag ctagctaaaa aaaatggagt gaaaataggc gcacatccag gtttcctaga
      301 tttaatagga tttggaagac gtgagatgaa aataacaaag caggaagcta aagattatac
      361 aaaatatcaa ttgggtgctt taatggcatt tgcaagttca aatggatgta atatacaaca
      421 tgtaaaacca catggagcat tatacaatat ggcagctaaa gataaagagt tagctatggg
      481 gatttgtgaa gctatatatg aagtcgataa agatattata ctgttggggc tatataatag
      541 tgaaatgata aattctgcta aagaaattgg acttaggttt gcaaatgaag tatttgcaga
      601 tagagcttat gataataatg gttttttggt tccaagaagt gtagaaggag cagttataca
      661 tgatacaaaa catgcaatag atagggttgt taggatggta aaagaaggaa ctgtagaaac
      721 tttaacaggt gaagttatac atatcaaagc agattctata tgtgtacatg gggacaatcc
      781 aaaagcaata gagtttgtca aagaaataag aaagcgtttt gaattagaag gtatagaagt
      841 ttgtccatta gaaaacatag aagtttgttc gtcagagaac atagtataag actgataaca
      901 tgagggggag ataaaatatg agtacaaaaa atattacaga taaaactaaa aataaaaaag
      961 atgtcggagc acttataggg gctgcattta taatggcaac atcagcaatt ggaccagggt
     1021 ttttaactca gactgcacaa tttacccaag attttggacc aaatttttca tttgtaattt
     1081 taattacaac aatccttttt ataggggctc aagtaaatgt atggagagta attggtgtat
     1141 caggacttag aggtcaggat atagcaaata aaataatacc gggattaggt tatttagtag
     1201 cttttttagt agctcttgga ggattagctt ttaatatagg aaatgttggt ggagctgcac
     1261 ttggtatgaa tgtaatgttt aatatgaata tgacattagg aactgtatta agtggattaa
     1321 tagctatatt tgtatttatg tcaaaaaatt caaattctct agtcgataag ataactaaat
     1381 ttttagcatt aggaatgata attatagttg gatatgtagc aatatcaaat catcctcctg
     1441 ttggagaggc agtatcaaga atggtaaaac cagaaaatcc aaaaggattg atattcccaa
     1501 taataactct acttggagga agtgttggag gttatataac ttttgctgga ggacatagat
     1561 taatagacgg tggaataaca ggagaagaaa atataaaaga aataactaaa tcttctcttt
     1621 tgggtatatt agttgccaca atgatgagag ttcttttatt tttagcaata ctggcagtgg
     1681 tttcaaaagg tcttcaactt gacccagaaa atccagctgc ttcagctttt aaatttagtg
     1741 caggagcaat aggatataaa ttctttggac ttgtattatg gtctgctgca attacatcag
     1801 ttataggagc tgcttataca tcagtttctt tcttaaagac actaaatcct tttatagata
     1861 aatatgaaaa atattttata atagcattta tagcaatatc aacacttata atggcattta
     1921 taggaaaacc agctacttta ttaattttag caggagcttt aaatggattg atacttccaa
     1981 tcacattagg aattatgctt atagcatcta aaagaaaaga tattgttgga gattacaagc
     2041 atcctacatg gcttttaata tttggattaa tagtagtttt aatatctgca tatacaggaa
     2101 taacttctct tagcagccta ggtgctttat ttgcttaatt tagaagaggt gacatttatg
     2161 gaaactagat acttgatatc aggggataag gcagtggttg ctgaatttgg aaatgagata
     2221 tcagaagata taaacaaaaa agtaatttca tttatgagag caatagaaat atcgaatctt
     2281 aaaggtattg taacagaaat ggtaccaaca tataggtcac ttatgataag ttataatcca
     2341 ttagaaatag attttgatag tttgattgag aatttaaaaa aaatagaaga taatttggaa
     2401 agtatagtat taccaaaacc taaaatacat gagattccag tatgttatga tgaggttttt
     2461 ggtatagata ttaaaaacgt tgcaagctac aataatctca ctgtagatga agttataaaa
     2521 atacacacaa gcagagagta cttgatatat atgctagggt ttactcctgg atttccatat
     2581 ctaggtggta tggatgaaag aatagctaca cctagattag aagtgccaag gactaaaatt
     2641 tatggtggga gtgttggaat agcaggttcc cagactggtg tatatcctat tgatagtcct
     2701 ggtggatggc aaataattgg taggacacct ttaaaattat atgatgaaaa tagagaagaa
     2761 cagattttgc ttagagctgg agactttata aaatttgttc caataacatt agatgaattt
     2821 atagaaatcg aaaaaaataa cttaagtatt taaatatcgt tatagtacag ggagtggtat
     2881 atatgggatt caaagttgaa ttaggaggtt tccaaacatt aatacaagac agaggtagag
     2941 ttggctatgg tcaatatggt gtatctggat gtggagcaat ggatgagtat gcacatagag
     3001 taggaaatat attagtagga aattctgaag atgaggcttc tttagaagtt ttaatgttag
     3061 gtcctacaat tacctttgat gaatatacgc aaatagcagt aactggggga gatttagggg
     3121 ctaaaataaa tggaaaagaa attcaaaact ggaggtcata tcaaataaat ccaggtgatg
     3181 tattatcatt tagaggtgtg aaatcaggtg ctagagccta tgtgtcaata gctggtggaa
     3241 tagatgttcc acttgcaatg ggaagtaagt caacttatac aagatctaaa attggcggat
     3301 ttgaaggtag agcacttaaa aaaggagatt atataaatac ttttattcag gaaaaagatt
     3361 ttacaataaa taaaaaatta aattcaaaat atattccaac ttatagttca gaaatcgtac
     3421 taagaattgt aaaaggtcct caatttgatg ctttttctaa cggtgaagta gagaaatttt
     3481 tatcaaataa atacaaagtt acgaatgaaa tagatagaat gggttgtaga ctagatggag
     3541 agagtataaa gcaccttaat ggagcagata ttatatctga tggtatttct tatggagcta
     3601 tacaagtacc tggtcatgga aaaccgatta taatgttatc agatagacag actagtggtg
     3661 gctatactaa gatagggaac gttataagtg tagatttgta taagttagca caagcaaagc
     3721 caaatgatgt agtaaaattt gaacttgtag atatatatga agcacataga ctattgagag
     3781 agcaagaaga taagattcaa gatatctaca agagtatgaa aaatataaga gttgtaaagg
     3841 caaaagtatt aaatgatata gcagtttaat ttaattttaa caaatacaat ttttattata
     3901 atttataata taacttacaa atagctattt actatttacc ataattaaga tggtttagat
     3961 tagtcaaata gctatttttt tagtttgagt aaatatttta aatttaaata aatacttcaa
     4021 ttttaaaata aaagcaatat ttaaatttat tatattatct acaaaaagta ggtgtgttat
     4081 gttgagttta tttagatttt attggccaaa ttggaggtga tataactcca tatccacgag
     4141 ctattgtatc tttacttgga ttgatgattt tatgtggtac attagctttt ataaatgttg
     4201 agtcacctgg atttaatcta actacctttt cacccataca aatatcaacc acaccactcg
     4261 ttaccaccat aatttcttct gatgcatgat agatataatc tttagcatcc tcactttctg
     4321 gtttaagttc aaactctaca aataaaattg aaggtgtaaa ttcagttgta ggcattggag
     4381 atactatctc gtaaaacatt tcactctttg gaaatttcat cataactcta tcttcttttc
     4441 taattactaa atcatcagta ttattgtcat ccataaacat atataaagga gtgtctaaaa
     4501 cttcagcgat ttttctcaat gaagacaagg atggttcaat agcatctctt tctatttgag
     4561 agatataact tatagataaa tctgtcaatt tggaaacatc acttaatgtt tttttcttgc
     4621 tttttcttat atttctcatc ttttcaccta gcataagaac ctccaaataa taaaattaat
     4681 ataaaatatt cgcttaaaac aaagtaatta tagcacaaca tagaagtagt ggcaataaga
     4741 atacatagta gattttgaca attaaaattt aattaaatcc aaataaaagt ctatgaaaat
     4801 gaattattgt taaattaata taaagaaaat aataataaaa aaatttaata gaagagtatt
     4861 aattgaatta agattatcac attatatttt ggaaaagagt aaattttcag taaattatct
     4921 tgacatatat attgaagtaa taatataatt taaagtataa aattttaaaa attgaattat
     4981 aacttcaaaa aataaggagg gtaatatgaa gataacagat atcaaatttg aaaagcttag
     5041 aattaaatta aaaaaacctg tagtagtttc atttggagta attgaatatg gagaaagtgt
     5101 aattttaaaa attgaaactg atgaagggta ttgtggattt ggcgaagcag cacctcttgc
     5161 agcagttaca ggtgaagttt tggataatgt tctttcagta ttgcttatgt ttaaaaaaga
     5221 gttaataggc aaagacccac tagatattga aaccatacat acaatcatgg atggaattat
     5281 tataggtaat acttctgcaa aagcagctat tgacatagct ttatatgata ttaaaggaaa
     5341 aattatgaat gcacctcttt ataaagttct tggaggattt gatagtaaag tccaaacgga
     5401 tattactata agtatagata agccagaaaa aatggcagta gaagctttag aaagagtaaa
     5461 agaaggtttt aggatattga agcttaaagc aggtataaat cctgaagacg atattgaagc
     5521 tgtaaaatta attagagaag ccgttggaga gagtataaga attagaattg atgcaaatca
     5581 aggatggaac gtaaatagca gtataaatac tatcaaaaaa ttagaagaat ttaatgttga
     5641 tgcaattgaa caagcacttc cacattggga tttagatggt actgcttaca taagaaataa
     5701 aagtaataca aaaattatga tagatgaatc gttacattca ccaatagatg caattaaggc
     5761 tataaaaaag aatgcagttg atacatttaa tattaagctg atgaaatcaa gtggaatata
     5821 tcctgctata aaaatcaaca acatagctga ggcaagtgga gtaaattgta tgttaggatg
     5881 tatgctggaa acgagaattg gtataacagc agcagcaaat ttaatagcat ctaaaaagaa
     5941 cataacagaa gctgaccttg atagttttat gttttgtgaa gaatcaaaag atatatctgg
     6001 aggatttgta atggatggag atattatgaa tcttgtcaat aaaccaggtt taggaataga
     6061 agtaaactta tgataaattt ggaggttaaa tatgaataca cttttttcaa atccaggtag
     6121 tatacttgct gtaatgacat caatgattgc attgggtttt tacttacaaa gatacaaagc
     6181 cattaaaagc ttaggaccag cacttacaat aatcataatg ggaataatac tttcaaactt
     6241 aaaagtagtt ccagtaagta ctgagttata tggaactatt tcaacatatg ctatcccagt
     6301 ttctatgact ataatgttga tgagtgttga cttaaaagaa atgacaaaat tatcaagaga
     6361 acctttaatt gcaatttttg tagctgtatt aactgtaagt atcatggcat ttttatttgg
     6421 attagtattt gcagaaaaaa tatcagaagg ttggaaggtt gctggaatgt ttgtaggtac
     6481 atacactggt ggaagtgcta atcttacagc tattggaaca ggccttaatg taagtagaca
     6541 aactcttgct gcagcaaatg cagcagacta tgtaattgga gtaccaacat tgatatttat
     6601 gtttgctcta ccagcaatat taaaaaattc aaagaagttt aaaaaacttt ggccatatca
     6661 tgtagaagaa acagaattag aagattgtca aaatgaagaa tttatggagt caaaagaatg
     6721 gagtattaaa gatatagctt ggatgttggc tataggtttt gttgttacag aagtttcaac
     6781 aatacttgct ggatatttca attcaagttt tagtagtgca gcaagaatac ttttagttac
     6841 aacaatttca attataattg ctcaattaaa gccagtaaag aaactaaagg gaaatttaga
     6901 tttaggatta tttgttgcat tattcttttt atgtacaata ggtttttcag tagatataaa
     6961 agagttttta ggttcaactt ttactataac actttactgt tttagcataa tttttgcttc
     7021 atttgttttt catttaggca taactagact tttaaagata aagtatcaat atgtaatact
     7081 ttctatagta ggagcaattg ctgatggtcc tacttcagct ttagtagctg cttctgcaaa
     7141 gtggaattcg cttgtaagtg tagcagtcgt aatgggagta attggtggag tattaggaaa
     7201 ttatgcaggt atatcagtgg catatgcaat aaagatgtct ctaggattat aggggggagt
     7261 ttaattgata aaatttaaat tatacatatc tggactatac agtggaaatg taatctttga
     7321 tggcgattta ttaattgaaa agttgaatcc ttttacaaat aaaatagaaa gtttaaaacc
     7381 aataagcaaa gaagaaaata catactattt aaatcttaca aaaatagatt taaagtcttt
     7441 atttaataac tttgatgtat atactaaaag cttagtaaac acagataaag acacagttat
     7501 aaataactta ggagaaacgc attctaaact taatgaatat atatgggttc aaagaaacaa
     7561 aaagttccca ctagatataa ttattgtaga taataaaata gtaggtttta tctgtttatc
     7621 cagagaaact tgtactatat taattatgga tggatatgaa gagtatactg tattaaaaga
     7681 atgggaaaaa acccataaaa atgaagagat atattctata agatttggtg gaaattatat
     7741 gattgatatg aaagatggta taaaactttc tacagatgta tatctccctg attttgtaga
     7801 ttctaccaaa aaagcaccaa caattcttat gagaactcca tatggaaaag aaaatgataa
     7861 agaaatatat tataaatatg tacaaagagg atatgctgtt gtaatacaag atgttagggg
     7921 aagaaatgaa tcagaaggta agtgggaacc tatgattcat gagagggaag atggagatag
     7981 taccataaat tggatagtat cacaggaatg gtcaagtgga atagttggaa tgcttggagc
     8041 atcatatctt ggttatgtcc aatgggcagc tgcatcttca ggcaataagc atcttaaagc
     8101 tttagttagt atagtaacat caggaagccc ttttattgat ataccaagaa aaggaggagc
     8161 ttttgtatct ggtatgcttg catgggcatt tatggtatca agaaataaag ttgatagaag
     8221 taaaatggta agagatgatt gggatgatgt tttaaacata cgaccaatag aaaatatacc
     8281 tgttgaagct cttggatata gaattgaatt tctggaagaa tggttaaaga gagtagaaaa
     8341 agatgagtat tgggatttga tggattggca tttacaaaaa gataaaataa atgtacctgc
     8401 attggttgta tctgggtggt atgatgataa tagtatggga actacagagg ccttagatgt
     8461 tataaaggac tatgaaaaag gaaagagaaa ggctattcta ggtccatgga tgcataattc
     8521 aaatacttta agagatataa atggtatttc tttaggaaat agttcattaa gatatgattt
     8581 ggattataat tatctattat ggtttgataa atacttaaaa ggtatagaaa ataacataga
     8641 tacgactgct ccagtagagt attactctgt aggttttaat aagtggaaga cagaagaaaa
     8701 ttggccaata ataaataaga tagataaaag tatgtatcta ataagtgatg gaaatgctaa
     8761 cacatcttta ggaaatggta gattagtatt tgataatgat ttagaagaaa agtatgatag
     8821 ttatatatat aatccaaaag acccatcagt acaactaatt gatatgtcag agaatgaagt
     8881 tggagttcca aataactaca aagatttaga aaaaagaagt gatatgttgt gttatacatc
     8941 agatgctttt agtgaagaat ttactgtaac aggtgatata aagttagagt tttttgcatc
     9001 aagttctgcc aaagatactg actgggttat aaaaattatg gacgtggatt tagatggaaa
     9061 ttcaattaaa ttagctgatg gtatacttag tgctagattt agaaatagtt tttataagtc
     9121 agagtttatg gaagaaggag agatttataa gtttacgatt ataacttcaa aaatatcaaa
     9181 cacgtttaaa gttggtcata aaattagact agatataacg tctagtgcta aaaactttat
     9241 tttccaaaat agcaacacga aagagggata taatagtatt gaatatatag aagcaaagaa
     9301 tacgatttac catggaggta aatatccatc taaattgata ttgccaatag aaaataagta
     9361 aatttatata gattaaatag tgaggaggtg tctcaaaatg aattttttag ttcgtgagac
     9421 actttctttt atacgaaatc aaatatattt tcatcatttc aacaatgaaa aagaggctta
     9481 tctctctgtt ttgagatagc ctcctataag ctaaattgat tattttgtag ctgcaacaac
     9541 gctcatttca actaacaaat gctctctagc aagtctagct tcaacacaag ctctagcagg
     9601 ttcaaatcct ttttcaaccc aagcatccca aacagagttc atagcttcaa agtctttcat
     9661 atctcttaaa taaatagtaa cagatagcaa atgttgctta tctgaaccat atttgtttaa
     9721 taaatcttct attttagcta aaacttcagt tgtttgttca attactcctc cttcagcatg
     9781 agtttgtcca cataaataaa ctgtattgtt atgaactaca gctctactca ttcttccagt
     9841 tccttcatat ctctttattt ccatatttta aatacctcca tagttaagaa atctattaaa
     9901 aatctaaata ataaattaaa aaaagtctat actatataaa attatattat atattctgat
     9961 aataatccat aaaaacatat aactttttag ctataattat tattgttatt aattgaaaag
    10021 acttattagt tacttgacat agtttacact tttaattata gtataaattg gaatatttag
    10081 ataattatta tttaatgtat aagctaagtt tttaattaat atggctttat acatttatat
    10141 agaaaagagg tacgtatgag tagcacaatc aaatcaaatc gaataaaagg tataatgttt
    10201 ataatagcat cagcgcttgg ctttgcaatg atgtctgcat tcgtaaagct agctggagat
    10261 ttaccaagtt ttcaaaaagt gttctttaga aatctggtat cagctataat agctttttgg
    10321 cttatcataa aacataaagg aagtctaact ggtaaaaaag aaaatttaaa agtactttta
    10381 tataggtcaa tatttggaac tctaggagtt atatttaact actatgcaat tgacaagttg
    10441 atgctttcag atgctaatat gctaaataaa atgagtccct tccttgtagt aatattttgt
    10501 gcaattgctc taaaagaaaa aataaatgta aagcagatag gagctattat aattgcattt
    10561 ataggagctt tattcataat aaaaccaaca tttagtgtag aagtaattcc atatcttggg
    10621 ggaattgcag gagctgtatt tgcagcaatg gcatatactt gtttaagagt tcttggagat
    10681 agagaagatt actatacaat tgtatttttc ttttcagttt tttcattagt tactgtaggg
    10741 ccaattgctt ttgcagtata tgaaccaatg acccttatgc aacttatata tctattacta
    10801 ggtggagtat ttgcaagtct aggtcaattt ggaataactt tagcatataa atatgcacct
    10861 gctaaggaaa tctctatatt tgactatagt aatataatct tttcagcaat tttaagtata
    10921 ttcttattca atgtctatcc agatatgtta agtgttgttg gatatttaat agtattttcg
    10981 gcagcatttt atatgttttt atacaataag aagttagata aattagacaa agaaaagaag
    11041 aagtaaacta gataaaagtg tttttataat ttgtattgaa tatttaagtc tttgtattaa
    11101 aattaaagaa aatttaatta aaaatgtttg gtatattaac ttataacagc agttaaaatt
    11161 gaaaaattat aactgctgtt tcttttatta aaaatatcca aatgtctgta aaaatagact
    11221 ataatagagt agtataaata atataaaggg gataaattaa agtatggaaa aagtgaaagt
    11281 tgctgtgatt ggagcaggta atagaggaac gtatgcatat gctccatata tttatgaaaa
    11341 ttctgatata tgtgaaatag ttgctgtagc agaacctaaa aaaggaagac gagaactatt
    11401 tacacaaaaa tataacctag atagtaaaaa tgtatttgaa actttagaag acttttttaa
    11461 acatgacaag atggcagatg cagtaataat agctacaaat gatgatagac attatgatgt
    11521 agcaaaacta gctcttgaaa aaggttatca tgtactatta gaaaagccaa tgtctaatag
    11581 tttagatggt ttggtacata tagatgactt gtgtgataag tataaagata agatttttat
    11641 gatttgccat gtactgagat attctccatt ttataataaa ttaaaagaaa ttgtagaaag
    11701 caaaaaatta ggagagctag ttagtataca atataatgaa aatataggat attggcattt
    11761 tgcacatagc tttactagag ggaattggag aaatagcaat gaaaccagcc ctttaatttt
    11821 agcaaagagt tgtcatgaca tggatatttt gttatattta gtaggaagta gatgcaaaaa
    11881 aatatctgca tttggaagct taaagcatct taataaccaa aatgctagtg gagagatggc
    11941 acaaaattgt cttcagtgtt tagttgaaaa aaaatgtcca tattcagcaa agagaattta
    12001 tttagaaaaa gatagaagta taaatagagc tgttcacata aatccaactg aagagaatct
    12061 attaaatata cttaagacaa gtccatatgg aagatgtgtt tacagatgtg ataataatgt
    12121 tgtagacaat atggtaaata tattacagtt tgaaaatgga gtgacggcta cttttaattt
    12181 atgtgcattt actaaagaaa atggaagaac tataaagtta atgtttagtc atggtgaagt
    12241 tggtggggat ttaaataaaa atgagataag aattaaaaaa tttggaaaaa acgaagaaat
    12301 agttatgaat ccatcaaatc aacagaatat ggtagaggaa tatgatagaa atcttatagc
    12361 tgaatttatt aaattagttt caaataaaga actagaaaag ggtagagtag cagcaaaaga
    12421 agctatacaa agtcatgtaa tggcatttgc agcagaatat tctagggtca gtgatgaagt
    12481 agtgtatata gaagaatttt ttgatagtgc taagcaaatg actaaggaga tagaagaaac
    12541 tatattttaa ttaataaaaa tttattaaat aaaataaaga tagattaaat aatagaaata
    12601 cgaaaatatg tgttctatta aggaatctat cttttttatt aaggtttttt cttatcccta
    12661 tcaaaaaaat ccatacattc tgataaaaga taagaaagta aagcgaataa gatttctgta
    12721 cttaaaggaa tcgttactga atccataaag gtatcaatta tgttcatagc tagaaaagtt
    12781 agtataaaat caattataaa ttcgcaaagt ttatatattg agtatggtaa tttgtttaca
    12841 tatctgaata tatctaaaat agtagtgcat agaaaatcaa ttggagtggt tatacaaaaa
    12901 tatattaaaa agaaaattaa tacagctcca agagatttat attcaaatcc taagaatgat
    12961 aagaagccaa ttcctataac cgtgaaaata caaaaagtta taccaaaaaa aacagcaaaa
    13021 aataaaaata cagaaataaa ttcgcctaaa ggaatccttc ttttattact tatcttaatc
    13081 aactccttaa tttatgatat gaaatttata taaataataa cattcttaaa aattcataat
    13141 agtcctctgt ttcaaatagt actgtttgag aatctatttt tttcatattt ggataaaaag
    13201 aggccttaaa agctttataa tgttctctaa attttatttc tatcttaaat ttatctggca
    13261 attttattaa gtgcctagat aaatctccag ataaagcttt tttgacacct ttttcaattt
    13321 tttttagtgc aaggtttgga tggattgaaa ttgaaccatt tccaattcct tttgacacag
    13381 gaacagtgac tatatttgga tttagttttt ttgcactctc acaaagcatt tcatctccac
    13441 ttaaaaatgc tacaggaaca ttataatatg cagcaatata agcatttata gtaaattcag
    13501 atgctatttc atcatttatt ttaaaatagt cataatcaat atccatagta tgagaaagtg
    13561 gattaccatt acaagtagct ccagagtgat agccggtgaa tatacatgca tcaaagcttt
    13621 catcaattcc agccatcata actagtgggt ctttggtcca tcctctaagt attttgacgt
    13681 tttcaggtaa aatatttgga ttaatatttc taccactgtc atgtgcatct ttaacaagta
    13741 tttcatgtac accagattga gtagctccat cacatgctgc tgttatctct ttactcattt
    13801 gtgttctata gtgttgtgaa aattcacatt caagttttgt ttcatcccaa tttacaatac
    13861 cacaagtacc ttctatatca gcacttaaat acaatttcat aatatatacc cccttatgtt
    13921 tattattgat gataataaca gataacagaa aatacataat caataaaggg cataaaaata
    13981 ttatgtctta ctattaatta ttattaaatc tgtaactgaa aaataactaa attggtatta
    14041 aaaaatataa cacta
//
LOCUS       DACTGW010000090        13954 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.090, whole genome shotgun sequence.
ACCESSION   DACTGW010000090 DACTGW010000000
VERSION     DACTGW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.090"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(129..965)
                     /gene="nadC"
                     /locus_tag="I9573_002039"
     CDS             complement(129..965)
                     /gene="nadC"
                     /locus_tag="I9573_002039"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAT4796659.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            complement(946..2247)
                     /locus_tag="I9573_002040"
     CDS             complement(946..2247)
                     /locus_tag="I9573_002040"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAT4796660.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            complement(2260..3174)
                     /gene="nadA"
                     /locus_tag="I9573_002041"
     CDS             complement(2260..3174)
                     /gene="nadA"
                     /locus_tag="I9573_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAT4796661.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            3592..5049
                     /locus_tag="I9573_002042"
     CDS             3592..5049
                     /locus_tag="I9573_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HAT4796662.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            5272..5700
                     /locus_tag="I9573_002043"
     CDS             5272..5700
                     /locus_tag="I9573_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796663.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            5760..6068
                     /locus_tag="I9573_002044"
     CDS             5760..6068
                     /locus_tag="I9573_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HAT4796664.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(6215..7237)
                     /locus_tag="I9573_002045"
     CDS             complement(6215..7237)
                     /locus_tag="I9573_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAT4796665.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(7412..7918)
                     /locus_tag="I9573_002046"
     CDS             complement(7412..7918)
                     /locus_tag="I9573_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAT4796666.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEMYELLYE
                     IDNNKLNI"
     gene            complement(7977..8678)
                     /locus_tag="I9573_002047"
     CDS             complement(7977..8678)
                     /locus_tag="I9573_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796667.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKRSN
                     EHKCKSSIVIKNEQN"
     gene            complement(8896..9966)
                     /gene="buk"
                     /locus_tag="I9573_002048"
     CDS             complement(8896..9966)
                     /gene="buk"
                     /locus_tag="I9573_002048"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HAT4796668.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            complement(10019..10594)
                     /locus_tag="I9573_002049"
     CDS             complement(10019..10594)
                     /locus_tag="I9573_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HAT4796669.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            complement(10596..12383)
                     /gene="iorA"
                     /locus_tag="I9573_002050"
     CDS             complement(10596..12383)
                     /gene="iorA"
                     /locus_tag="I9573_002050"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HAT4796670.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEMNDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            complement(12444..13703)
                     /locus_tag="I9573_002051"
     CDS             complement(12444..13703)
                     /locus_tag="I9573_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAT4796671.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVEEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
BASE COUNT         4533 a         2539 c         1546 g         5336 t
ORIGIN      
        1 atttttatta aattattgat tgattttttt atatttttat taattttaat gaataaaaaa
       61 tagactcctt aaatttataa aacaaattta aggagtctat tttaattaag tttatttcaa
      121 tataaaactt atatatttct caaattcttc atacttatat cgagagcttt aacagaatgt
      181 gtaagcgctc ctacagaaac aacatccaca ccaatttttc ctatatcttc tatagtatct
      241 aaatttacat ttccggaaaa ttctacaatg gctcttccat ttataatatc tactgcttgt
      301 tttgccattt ccaaactcat attatccaac attattatat ctgcattagc ttctattgct
      361 tcttttacca tgtctaaagt ttccacttcc acttctatct ttctaacaaa agaagtattt
      421 tttctagcta attcaactgc cttcttaaca ctaccagcag ccccaatatg gttgtcttta
      481 agcaatattc catcagataa attaaatcta tgattgcatc ctccacctac tttaacagaa
      541 tacttatcta atattctaag attaggtatc gtttttctac tatctaataa ttttgtattt
      601 gtaccttcaa gcttttcaac atacctactt gtaatagtag ctataccact cattctctgt
      661 aaataattca atgctacacg ctctcctaca agtaagtttc tagtactacc tgttaataca
      721 gctatctttt cacgattctt tactctatca ccatcatttt tatataattt tacatctaca
      781 tcaccaagta tctcaaaaac tctcttaaat acacctaagc cagctattat accatcttgt
      841 ttgcaaatta aatctacagt agatttagaa ttttcatcca caatagaatt agttgttata
      901 tcttcacttg gaacatcttc tatcaaagca tcctgtatca ttctatcaat aatcaaataa
      961 ttcatcgcca aaatcctcac ttgttctttc aatttctttt atgactaata ttttattttc
     1021 tttttttacc tgttctatat ctatatccat atctggtaca tctataaact taacttttac
     1081 attatctata actgagttta tatttttagc tgctcttttt gaaaatacaa gcccttcaag
     1141 taatgaatta cttgccaatc tattggctcc atgaacacct gtacaactaa tttcaccaac
     1201 tgcatataaa ttttccatag atgtttttga atccaaatct acatgtatcc cgcccataaa
     1261 ataatgttgt gcaggagata cctttataca ttctttcgtt atatctgttc ctctagctaa
     1321 acattcatta taaataaaag aaaatctatt aattaaatat tcactatcta aatgtgttgc
     1381 atctaaatac acataaggta atttatcttt ttccatttgc tcaaaaatag cttttgaaac
     1441 tacatctcta ggaagaagtt catccacaaa tctttcttta tttttattta aaagtttagc
     1501 tccctctcct cttaatgatt cagatataag cattctccta ccttcattat tttcttcata
     1561 aaaggctgta ggatgtattt gaatatattc taaattttct atttttatat tatttcttaa
     1621 agctattgca attccatcac ctgtaagatg tctttgatta gtagaatttt taaaaagccc
     1681 accaattcca cctgtagcta aaactactat ttttgaaaaa acatgaatct cttttttaga
     1741 tttaaaaatt ctagctccta tacatttatt gccatctttt aatatatcta aaagatatgc
     1801 atcttctata agagttatat tttctctagt tttagcttct tttaataaag ttttaaaaac
     1861 aacttctcca gtattatcct tactatgaac tattcgattt acactatgtg ctccttctct
     1921 tgtgtaatct atctctccat tttctttgtc taaaggcata ccatattcta ctatctgccc
     1981 tacattttct atagattcat ctgcaagtac ttgaactgct tcaactctat ttttatactg
     2041 cccagccttc attgtatctt ctacaaagga ttctatatca tcaatatttc tagcagttga
     2101 aattccacct tgtgcaagat atgtattgtt gttttcaatt gttgatttcg atactactag
     2161 cacatttaaa cttttatcta aatttaatgc acagtataat cctgaaactc cacttccaac
     2221 tattaaaaca tcttgttcta aattcataag tctatccctc tatccttctg ctaatttgtg
     2281 catgttaagc agtgaattta aagccttttc catgatttca tcttctacaa taacttcatt
     2341 ttccatattt ttaagtgtac tatataaatt ttctaatgtt gtctttttca tatctgtaca
     2401 tagtattttt ccaccaggaa tataaaagtt tttattagga ttatttttct ttaattcatg
     2461 taatatacct tcttcagttg ctattataaa gtctttgttt ttactattag tagcatattt
     2521 tattattccg gatgtactac ctacataatc tcctaaatct cttatttctt ttttacattc
     2581 tggatgtact aatacttctg catttttata cttatctttt aattcaatta tttcttcagc
     2641 tcttatattg ttatggtatt tacaatatcc gtcccaaaat ataaagtttt tatctggaaa
     2701 ttgttctgca atatatcctc ctaaattttt atctggaagg aatataattt ctttattatc
     2761 tatgttttct agtattttaa ttgcactaga agatgtaaca gaaacatcac aatgtgcctt
     2821 aactttagct gtagagttta tataacatac aaccaaagca tttggatact gttgttttaa
     2881 ttttatcacg ccttcttcat ctaccatatc tgccatagca cacccagcgt tacttactgg
     2941 catcaataca gttttttcag gtgataatat cttagcactt tctcccataa accttactcc
     3001 acaaaataca accacttctt ctttacaatc tctagcaatc tcacttaaat aatatgagtc
     3061 accaactgca tctgctaatt cttgtatttc tggtggttga taaaaatggg caagtattat
     3121 tgcatttttt tctttcttta gctctttgat ttgatatgtt aaatctttat ccataattgc
     3181 accgctttct atatgtacta gtgtatatac acctgtaaac ccattatatc acttgactat
     3241 tctttcttca agaattttaa acctcttaat attgttaaga tatagacatt tatattctca
     3301 attgattttc cttttttctc catctattta aactaatttt tatatttttt aatttttttg
     3361 taaaatatta tcagaatttt gtttatattc tttattgcta taacattgaa aatattacca
     3421 aatacctgta tactaagtac atggttatgt atatgtattt atttctgaaa ttatattagt
     3481 tgtaatactt tatagaattt gtaggttgtc cagaattaaa ttcaatataa atctacacat
     3541 ttagataata tgtatacaaa gaccaaaaaa ttttataaat ggggtgatat tatgacaaca
     3601 ttcttaatcg gcttggctat attactaata ggaggtgctt tatacggtgc ttattgtgaa
     3661 aaagtatttg gcccagatga cagaaaaaca cctgcacttg ctcaaagtga tggagtagac
     3721 tatgtcccaa tgaaaaaatg gaaaaatagt ctaattgagt tgcttaatat agctggtaca
     3781 ggaccaattc taggacctat ccaaggtata ttgtttggtc caatagcatt catactaata
     3841 cctattggct gtgtatttgg aggagctttg catgactata tgagtggaat gattagtata
     3901 agagaaaaag gagcacaaat gccttctctt atcagtagat ttttaggtaa taaagtattt
     3961 caagtctata atatattcct ttgtctttta atgcttttag ttggtgcagt ttttatttat
     4021 acacctgggg atttagttgt tactcaaatt ttaaatatga aatcgactat aaataatcca
     4081 gttgtctgga tagtttatgg acttatcttt ctatattact tgtgtgcaac actctttcca
     4141 attgataaaa tcataggaaa ggtatatcca atatttggag ctattctact tttatctgct
     4201 gctggtgttg gaattggaat ctttacacaa ggttatgatt tagctaattt aagtcttgca
     4261 aattggaaag gtattcatcc tgatggtatt cctcttattc ctacattctt tgttacagtt
     4321 gcttgtggta tcgtttctgg attccactca actcaagcta ctcttatagc aagatcagtt
     4381 tcaaatgaaa aagaaggaaa aactactttt tataatatga tgattttaga aggtttaatt
     4441 gcaatgattt gggctgcagc tgctatgggt atttacaata aaggaatacc taaggaatta
     4501 gtaggttctc ctgatgtaat aggtttggtt gcaagagatt tacttggttc tattggtggt
     4561 atcatagcta taataggtgt aattgtttta ccaataactt ctggagatac agctttaaga
     4621 tctttaagat taatgcttgc agattacttt cattatgacc aaaaggaaaa gaaacatcgt
     4681 gttatattgt caatctgtat atttattcct gtcatagcca ttcttatatt tgcaaagctt
     4741 agtgcttctg gatttaatat attgtggaga tacttctctt ggagtaatca aactattgcc
     4801 atatttgcat ttgcaatgat tacagtttac cttataataa aagaaaagaa ttatattata
     4861 agcttaattc ctggtatgtt ttattctttt gttatattca gttatatatt taatgctcaa
     4921 attggattta acttaaacat aaatatatca tatgttttag ctgccatatt tactgtatta
     4981 tatgctattt taactgtacg ctctggtaga aagttaaaaa gcaaagcgga tactaaattg
     5041 gctgactaaa tctaaataaa ataatactta gaataaattt tattaaaaaa tgataaaaaa
     5101 gtaggcaatc tcaagttaga tttgcctctt ttttatttaa tacagtaata aatacttaag
     5161 tatttactcc ttattggaat atttatccta aatctctaat ataaataaat aaaagtttta
     5221 aaataatcta ttcatttata tacttaaaat aaattttttg gaggaatact tatgaataat
     5281 aaattacatg aacttgaaaa aaatctacca gaaacatctt gttctttttg cacacatctt
     5341 tcatttaagg ggcctagcct agactataga tatgatatta gatgtgttat ttcagataca
     5401 aagcctgatt ttagaggatg ttgtgaatat tttgaaccag aatatacaga attaaataca
     5461 ggagatttag acaatctata cataaacttt cttgagacat gcctcaaggt aaattataat
     5521 gattacatca attctatgta ttggaagtta tttaaagaaa gaaccctact agaaaacaat
     5581 tttaaatgtt ctatatgtgg atcaactgaa aatgttgatg tgtatcatgt taataaaaat
     5641 ttaggaaggg aaacttccaa agatgtagtt gttaaatgtg ataaatgtaa ttgtaaataa
     5701 gtgtataaaa tttagataac ttcacataat aagtttaaat aaaacttagg aggttattta
     5761 tgaaaaataa taatttaaat tgtgctgcta ctaattgtgc ttataatact agtggatatt
     5821 gttatgctgg tagtataaaa gtagatggaa tgcaagcaac tactacagaa aatacttatt
     5881 gtgcttcttt tgaagacaaa tatacatctg gtataactag tcgttctaat gaaactaacc
     5941 aagttgatac agataacatt cattgtgaag ctgtaaaatg taaatacaat aaaaatgaac
     6001 tttgcaaagc tgaaaaagtt catataaatg aaagaaatgc aagttgtgaa acttttgaaa
     6061 tgaaataaat ttctttttat ttaattctat agtatttata caaagcaaaa ttgaacctat
     6121 caaatagatg tctttattaa tgatgtctat ttataggttc ttttttaaaa caattcataa
     6181 tttatatata aaaataaatt attattttaa tatattacct agaagtaaat ttaacaccca
     6241 gaatatccat aaggaacata aatattggaa ctccaattag taatccccat acacctaaat
     6301 aatgttctcc tacaagtaaa ataataaaaa caaaacaaac tggaagttta gttctattcg
     6361 acatcaactt agggtttaat atgtatgttt ctactgcatg cactatacaa atcataagta
     6421 aaactccaaa aacctttgtt attcctcctg tattaaatgc tattacagtt aatggaatca
     6481 aagacactat aactcctgct actggaataa gacccaacac aaaaatcata aatcctaaag
     6541 cccatatttg agggaatccc atgaaaccta agaaaatcat agaaacaaca gaatttataa
     6601 aggcaatcat aacttgtact ttcattactt caccaaaatt tttaacaaag ctctttccaa
     6661 aaaatacaag cgaattataa ataaaagaaa tcttactatc agaaagttta tctccaaaat
     6721 ttttaatttc atttttttca agtaaaagga gtaaactcag taaaaatgcc aataatatat
     6781 ttactccaaa acttccaact tttgttgctg ttgatgctat aagtagccct gcttgtgaaa
     6841 tatatttatt aaaatccagc tttgagacaa taggatacaa ttttggtcct aaagattgag
     6901 caaattgatt tacatcaaaa tcactaaaaa ccctactcag ctctgtaagt tgaactataa
     6961 ttattggtac aactgcatag ctaatcaata ctaacaatat agcaaatagt gcatataaca
     7021 ctataattat caatgaatct ggaatattta aagaagaaaa cttcttaatt cgttttcttg
     7081 tggctacaag caagttatag aaaataaaag tcataataaa tgttaaaagc accatattta
     7141 acatatacca aaatgtaatt agaataatta ctacgacaga tatagaaaca attttatatt
     7201 ttgaactatc acgtgccaat cctttgttat cttccatagt ttaaatcctc cccaaatttt
     7261 tatgacttta taatgactgc cctttttatt ttaatctcaa aaaacaagta gaaaaatact
     7321 aaaaataact tattaatttt cataaaacaa tattaaaatt tagagaagat acattttaaa
     7381 taatcttact tatactgaaa tctgctctac atcatatatt taatttatta ttatctatct
     7441 catatagcaa ttcgtacatc tctggatatt caaaaaacaa accctctttt agtaattgtc
     7501 ttatttcatc tgtgcttacc cactttatat cacttacttc ttcctcctgt aaaatagctt
     7561 tggatatatc atattctttt ttaactaagt atacatccca aagagtatct tcatgaatca
     7621 tacttctaaa aactttcatc tcatctttag ttatatctat ccctatttct tcctttgctt
     7681 cgcgaattgc tccttctaaa ctatcttccc cagaaacaat acatccagtt gtcattcccc
     7741 acatatttgg caatgtcttt ttacttttag accttttctg aattaaaatt tgagagttac
     7801 tatttaaaat ccacacttca actgccaaat ggtaataacc ttcttcaata gaattacctc
     7861 tttttataac atttcctgtt ttaattccat cagcattata taaatcccat aattccattt
     7921 caaaatctcc tgactttact ataaattaga taaactacaa atatatttta gcataattaa
     7981 ttctgctcat tttttattac aatacttgat ttacatttat gttcattgct tctcttttcc
     8041 ttccattctc ttatatcatc tataataaat ctatgtttca gcaactttgt gtcaaattta
     8101 aatattctaa acactatttg taatacatat cctaaaccta aagcacttac aagcgtacca
     8161 atacctacag aacctcctaa taaccaccca acaactaatg ctagtatttc aagaattgtt
     8221 ctaacaagac gtacagattt attagttttt ttctgaagag caatcataag cccatctctc
     8281 ggtccacttc ctaaacaaac acctaagtat agaaaacttg ctatagctgc caaaataatt
     8341 cctataacaa ccataaacac acctatataa gtgttgcttg catgaggtat cactccagaa
     8401 aatattacta aatcaataaa aattcctata aatgtcatgt tgagtaaagt accccatcct
     8461 atattttcac caaaaactgc atccaatata acaattacaa ctcctaccat tatagaagcc
     8521 tgacctatag ttatgcctag ctttatagat aaaccttggt gaagtacatc ccaaggcata
     8581 agacctacat gtgcattaat cataagcaca gaagaaattg caaatataaa aaaacctgaa
     8641 aataatctta tcatactttt taaacttatt tttcccatca aaaatcactc ctcataataa
     8701 taataacaat aaataccaat aaaatatata tataaatacg aaattttaca gaattattta
     8761 ttagattaag ataaaatttt aattataaaa agtggcaatc caaattttat acattttcac
     8821 ctatactcta agctttcgtg tataaaaaac ggatatacca cttttttagt atataaacat
     8881 ttttaatttt tcttattaat tttcactata aattctagct tcttcttctc catttagtac
     8941 tcttaaagtt ccaaaagcaa gtgattccaa ctcattttct cctgccataa tttctactgg
     9001 tgctataaac tcaactttct ttttaaccat tgaagtaaac atttcagaat atgcaattcc
     9061 acctgttata attatagcat ctacctttcc atctactaca gttgctaact ctccaatacc
     9121 ttttgctaac tgataagcca ttgcttcata aataattttt gcttcttcat ttccttctgc
     9181 tatcattttt tcaacttctc tagcatctac agtatttagg tatgaaacta taccaccttt
     9241 acctcttatc tttttagtca tttctctttc tgaatattga cctgaataac aggcagccac
     9301 taattttgta gcaggtactc ttcctgacct ttctggagaa aatggtcctt catcatcaga
     9361 tagcatatca accattcttc ctttttcatg aagataaata cttactcctc caccaatgtg
     9421 agctactatt aagtttaagc tcttataatc ttttccttta tccttagcat attttaaagc
     9481 cattgctcta gtatttaatg catgccctgc acttgatcta tccattccac aaagtccaga
     9541 tattctcgct acatctataa gctcatctac tgcaacagaa tcataaatat atgagttgat
     9601 tccaagaggt tctgatattg catgtgccac aacagcacct aaattggaag cgtgttcaag
     9661 tactggtcta tgtcttagta catcaatcat ttcttcattt actagatatg ctcctgattt
     9721 tactggtggt aaaactccac ctcttcctac tattgcacta agagatttta agtctatact
     9781 attttcttta agaatattca aaactgcttc cttacgcatt tcaaactggt cttgtatagt
     9841 attatatttt gcaatctctt cagctggatg gtctatcgtc ttcacaagaa tttgttcttc
     9901 tccatcatat actgctattt ttgtagaagt agaacctgga tttatggcta atattctgta
     9961 agtcataatt ttcttccttt caccttaatt tctatagtct atttatatct attataaact
    10021 atttgctaac catttccata ccttcatgga aagcttttat gtttaattca acaaattttg
    10081 gtttaacatt ttcacttata attttttccc aatcaataga ctctaattgc atagatttaa
    10141 ctagacatcc taataaaata acattcatta tttttgaatt tcctagttct tcagctttat
    10201 cagctgcatt tataactttt gcatttaatc tacttaactc tgcatctata tcactttctt
    10261 gatattcagc tcctccgatt aaaacagtca tagaatctat cctatgatta tttactaaag
    10321 cttttccttc tggttttaaa taattgaacc atcttaaagc ttccattttt tcaaaagata
    10381 ctaatatatc tgctccacca atttctataa ctggagaatg aacacaatct ccatatctaa
    10441 cttgtgagga aactgaacct cctctttgac tcattccatg tatttcactc atctttacat
    10501 catatccagc ttccatcaat cccatagtta ataatttact agcaagtata gttccttgac
    10561 ctcctacacc taccaaaagt atactcttag tcatattatt tttcctcctt aactattgct
    10621 tgttttggac aaacttgtgc acatactcca catcctacac actgatttct atctatattt
    10681 gataacttat tctctttatc aaatgataga gctggacatc cagttttcaa acataattta
    10741 catcctatac atttatcatg gtcaacttta cacttagtct taaatggatt attaaattct
    10801 tctatatctt ctttagagaa ttttttaagt acacatggcc atcttgttat tattactgat
    10861 ggttcatctt ctatagctaa agcccaatct agtgcttcat ttacttcttt taaattgtta
    10921 gggtttataa ctcttacatg ttctattcca caagctttaa ctaatccttc tatatcaact
    10981 tctttagttt tagcaccttg taaagtatac cctgaacctg ggttttcttg gtgacctgtc
    11041 attccagtta ttctattatc taatataacc gaaatagaat tacctctatt gtaaacaaca
    11101 tctaaaagtc catttattcc tgtatggaag aatgttgagt ctccaagtac acctaccaat
    11161 cttttttcat tatcatcctt catattaaga actttttgag ctccatgtgc agttccaaaa
    11221 gcagaaccca tacaaacaac ataatctatt ccattatatg gaggagcaaa tccaagagta
    11281 taacaaccta tatctccacc aactattaaa ttttttctct ttccaagttc atagaagaat
    11341 cctctatgtg gacatcctgc acagaaactt ggtggtcttg gtgttactaa ttcagatttg
    11401 tattctatag tatcatttgt ttttccaaat atagcttttc ttaaaacatc tggtgtcatt
    11461 tctccatatg gtggtattac atcttttcca atacaatcta ttccatatgc ttttatttgt
    11521 tcttctataa atgggtcatt ttcttctata acatatattt tatcaacttt ttctgcaaat
    11581 tcttttattt tttcataagg taatggattt gtaaatccta gtttcatata tgatgcattt
    11641 tttccaaaaa cttctttggc aaaattacaa cacataccag aagctataac acctattttt
    11701 gtatcattca tttcatagta gtttaaatca gtttcatttg agaatttttt aaggatttcc
    11761 attctttctt caacaacgcc ccttcttatt tgagcatttg ctggaacagt taccattttc
    11821 ttagcatttt taacatactc tttaatttca acttcttctc tatcatagca ctccactaat
    11881 ccttttgaat gacaaagtct tgttgtaact ctatataaaa ctggtgtatc gtatttttca
    11941 ctaacttcaa aagcttcttt tatcatatct ttagcttctt gactagttga tggttcaaat
    12001 aaaggtatct ttgcaaattt tgcatacatc ctattatctt gctcattttg agatgaatgc
    12061 attcccggct catctgctgt aataagtacc ataccaccat ttacccctgt atatgcataa
    12121 gtaaatattg gatcagctgc aacattcaaa ccaacatgtt tcatagaagc cattgttctt
    12181 gctcctgcaa ttgaaccccc tatagcagcc tcaagtgcta ccttttcgtt tggagcccac
    12241 tctgcaacta ttgcatcttt atatgttgct atattttcta atatttctgt acttggagtc
    12301 cctggatatg cagatgcata tttaacacca gcctcatagg ctccacgtgc aattgcttca
    12361 ttacctgtca tcaattgttt catataaaat tccctccatc tacaattatt aaattcatag
    12421 tccacccaat ttgttcaatg aaattaatta tttattttta cttctaaact ttcaagtgct
    12481 tcttttataa tacttggtgc tttcttacat ttatcttcag aaactgcaca aactgcaaat
    12541 cttaagccca tttttaatgg aactgcaaat aaattatgtt tagtcaattc ttcacatact
    12601 tcttttggat tatcacatgg tatacttaca aagaaacctg ctatatatgg aagaatttct
    12661 aatcctactc tttcagcttc ttctacaaat acatctgctc ttctagtaag catgtttttg
    12721 tatatttctt tttcatcctc atattctttg aattttttag ggtcatttac aatgttagat
    12781 aatactgcca tggcacttct attacaattt gaccaatttg ctctacatga atgaacacat
    12841 gagtaatgga actcttcagc tacatcttca cttgaagata tacaaattat agcccccatt
    12901 ctcataccat aagctgtaaa tccttttgac atactgaatc caaccaatac gagaacgttt
    12961 tcaggcaaat tagaaaattt cttaaaaaac tttctacata catcatcatc acctgcaaag
    13021 tcaatatatg ctgaatctac gaaaaatatt atctttttgt ctttatcttt tgcgacttct
    13081 tttgataagt ctaatatttt atcccactca tcatctgcaa cactatatcc agttggatta
    13141 tgcgctggtg tgtttataat tgtaaagact ctaccttgtt tttctgctat atttacaaat
    13201 ttttctttga acgactcaaa attaaacctt ctattttcat caaaaagttg gtaattaact
    13261 acttttctat tagcctcttc agctatactt acatatggac tccaaaacca atcagatgtt
    13321 aatacctcat ctccttcatt agtatagttc catacagcta attttattgc accacttcct
    13381 cctggtgaag ctaatactct tatatgtcct tctggtaagt aatctctgaa aaatactttt
    13441 ttaactgctt ctaagtagtc tggttgacct tcaagagctg catatgcacc tatctcacag
    13501 ttatctaatg ctttatattc ttcatataca gttttcattg ttattaattt tcctgaatca
    13561 tccattagag cgcctattgt tgcatttatt acattatcca taccaaaagc cttctctgct
    13621 gcttgagctc taccagatat gctaaatata acatcatttt cttttggcca cattgcatgc
    13681 ttaccaacca tactttgact cataccatca cccctaaaat tgtatttact cttacattat
    13741 atattaagca attactatgc caataaatcc cactttatgt aaaattgatt ttgtttagaa
    13801 ttattatttt tttcattatt aaattaagtg ttttaactat ttttcttata aatatatatg
    13861 cctgtctaaa aaaataaaaa tttacaaaaa agtaatgtta atttttctta acacttgttt
    13921 taaaaaatta acattacttc aaaataattt atat
//
LOCUS       DACTGW010000091        13945 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.091, whole genome shotgun sequence.
ACCESSION   DACTGW010000091 DACTGW010000000
VERSION     DACTGW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.091"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            110..622
                     /locus_tag="I9573_002052"
     CDS             110..622
                     /locus_tag="I9573_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAT4796672.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKIYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(777..1838)
                     /locus_tag="I9573_002053"
     CDS             complement(777..1838)
                     /locus_tag="I9573_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HAT4796673.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(1962..2855)
                     /locus_tag="I9573_002054"
     CDS             complement(1962..2855)
                     /locus_tag="I9573_002054"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAT4796674.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIHSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQNYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            complement(2865..4346)
                     /locus_tag="I9573_002055"
     CDS             complement(2865..4346)
                     /locus_tag="I9573_002055"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAT4796675.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKKFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            5487..6395
                     /gene="trxB"
                     /locus_tag="I9573_002056"
     CDS             5487..6395
                     /gene="trxB"
                     /locus_tag="I9573_002056"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAT4796676.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(6485..8308)
                     /gene="typA"
                     /locus_tag="I9573_002057"
     CDS             complement(6485..8308)
                     /gene="typA"
                     /locus_tag="I9573_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAT4796677.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            8523..8762
                     /locus_tag="I9573_002058"
     CDS             8523..8762
                     /locus_tag="I9573_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796678.1"
                     /translation="MEVTKIIIKYFASESDTFIKEEVCNFEELLKWSHKFIEGKKIED
                     VLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            8910..9101
                     /locus_tag="I9573_002059"
     CDS             8910..9101
                     /locus_tag="I9573_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HAT4796679.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            9392..11893
                     /locus_tag="I9573_002060"
     CDS             9392..11893
                     /locus_tag="I9573_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAT4796680.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNVLAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            12053..12721
                     /locus_tag="I9573_002061"
     CDS             12053..12721
                     /locus_tag="I9573_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4796681.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            12705..>13945
                     /locus_tag="I9573_002062"
     CDS             12705..>13945
                     /locus_tag="I9573_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAT4796682.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEKSELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNK"
BASE COUNT         5267 a         1811 c         2155 g         4712 t
ORIGIN      
        1 caataatata tttaaaaaag tattttattt tcaaaactag acttcattta taatttatca
       61 cgaaaataaa aggaatataa atataagaga atgagaggtt attagagata tgagtgattt
      121 taaagaaatt ttggcagaag agattaaaaa taatccattt ggattgatag gtaaagattg
      181 gacacttata acagcagaaa atgaaggtaa agtgaataca atgactgcta gctggggagg
      241 acttggagtt atgtggggaa aagatgttgc ttttgtagta ataagaccac agcgttatac
      301 taaggaattt atagataata cagataaatt ttctcttaca ttttttgatg aagattttcg
      361 taaagagctt tcatattgtg gaaaggtatc tggtagagaa gaggataaga tatcacagat
      421 tggatttaat atagaacact taaatgatac accttatttt aaggaagcta aaatggctat
      481 tatatgcaaa aaaatttata gtcagaaact tgaaccccaa tgttttattg ctgaaggtat
      541 agatggaaga tggtatccac aaaaagatta tcatacttta tatatagctg aaataaccaa
      601 tgtacttgta aaagaagatt agttataagg gttaatacta tctctaatta aattaaaatt
      661 tcaataaaag gaaattgaca caaaaaagct aaggacataa atatgtttat aaagattaat
      721 aaataacgta ttaaaactcc ttagcttgat aaaattttaa tttagttttc tttaaattat
      781 aattcagaaa tcaattcatt gataacatct tcaacagtac ttaatttatc aattttatag
      841 gcatcatttc cacaaaataa aagtgaattt tcaacatctc ctttaactgc attgattaga
      901 gcttgagaaa tacagtaagg agtttctttt ggattacaag gaatcaaaca attatagcac
      961 ttctttattt ctggacgatt tattttaact tcttctataa atttgtttct tatggcgcgt
     1021 ccaggaagac ctacaggact tttaactata tgaacatctt cttcactagc atttatgtat
     1081 gccattttaa agttctcatg ggcatcacat tcgtaagtag caacaaatct tgtaccaact
     1141 tgtacaccac ttgcacccat atctatatat tttttaacat cactactaga agttatacca
     1201 ccagcagcaa ctactggtat gtttctacca aatttttctc cataagtatt tgcaaccttt
     1261 aaaatttcaa caaattcctt atcatagtct atagaatcta tgttatcaag ttcctcgtta
     1321 gagtatccaa ggtgaccacc agcttttgga ccttcaacaa ctattaagtc agcagtagtt
     1381 ttctcttttc tatcccacat ttttaaaatt acattagcag cttttgcagt tgaaactatg
     1441 ggtgctattt ttacattact tccctttact aatgatggta gcttattagg taatccagct
     1501 ccagatatta taaggtctac accagcttta acagcttcct ttacgtatgt ttcatattct
     1561 ttcatagcta ccataaaatt aataccaata ataccaccat ttgagatttc tttggcctta
     1621 gaaatatgtt tttttatagc tcttaaattt gcatttatag tattagtttc aaaatcttcc
     1681 tcatcatatc caatttgaac tccagaaatt acaccaacac caccaagctt agcaactgca
     1741 ccagctagtg atgacctaga tataccaacg cccattccac cttgaattat aggaactttt
     1801 gcaactaaat ctctaattat taactctttt atgataatcc ctcctcaatg taaacatgtt
     1861 taaagaacta gattataata cttaatatta ttacctagtc ctaattatta tactacaaat
     1921 tataataaaa gagtagttat attttgtata gaagacgata attatattat atttaaaaca
     1981 gctccattag aatcgcaaca taactctttt atttcccaat cagattttag tttttgtagg
     2041 taatttttca tattatccac aaaactatca tcattttctt ttattaaaga cattatagta
     2101 ggtccagctc cacttaaaaa tactccaata ctatttaatt gttcagattt ttcaacaata
     2161 tcattgtagt tctcaattag agttcctcta taattttgat gcaatttgtc ttgacaagct
     2221 actttaagta ggtcaaagtt tttgtttagt aaggcagata ttagtaaagc aactctacta
     2281 acattgaata ccccatcaga atatggtatt tcctttggta aaacacctct agctttttct
     2341 gttgataact taaaattagg tatcattgca caaaatttaa gttcttcagg aatttttata
     2401 atatcataat gtatattctc attatcagta acagatacga tcatatttcc taaaatagca
     2461 ggagcaacat tatctgggtg accttctatt tctgaagcta tatttaataa ttgattttta
     2521 tctaaattag cacctgatag agcatttgca gctataactc cagctactat acaagaggca
     2581 ctactgccaa gacctctaca tataggtact tcactgtgga tttttatttt gataccagca
     2641 ggtatttttt ctggtttaac tctatcaaaa aagtacttca tagatgtata aactaagtta
     2701 ttttcgtttt tgtaagcatc ttcacaacct tctatttcaa gaccactttc aatttcttca
     2761 acataaaatt tattgtatat atttaacgca attccaagac aatcaaatcc tggacctata
     2821 ttagcacttg ttgcaggaac tataatctct aacataataa tcacctatac ctttaagaat
     2881 ttttttatag ctagttttaa ttcatctttt tcataaatat ctttatgtag tatttctgca
     2941 tttttaagat ttttaatagg ttttggaata tctattttag aaatacttga taatctatca
     3001 attatagcaa agtcatcaag gtctttaaaa tcacaatcta tagactttag aacatcttca
     3061 gaaaacttga atggacttgc agtacttaca ataacagttt ttgtgttatc agaagtttct
     3121 tttttatatt tttcataaca attgtatgca acagcagtat gagtatctat caaataatta
     3181 taatttttaa acacattgtt tatcgtgctt ttaacttctt cttcatttga atattctcca
     3241 tagaatgagg ctaaattttt ttccatatca tcacttattt tgtagactcc tttatcacta
     3301 aggtctgata acagcttatt tacaatatca gtatttctat ttgatatttc aaacagtaat
     3361 cgttctaaat tacttgaaat taatatatcc atagaaggag aagtagttaa tttcaatgtt
     3421 ctatttttat catatacacc agttttaaag aaatcataaa gaacattatt atcatttgat
     3481 gcacatatca atttgtttat tggaagaccc atttgtttgg cataatatcc agctagaata
     3541 ttaccaaaat taccagttgg gacaacaaaa tttattttat catttaaatt tatctcacta
     3601 ttgctaacta atttcatata tgaataaaaa tagtatgcaa cttgtggaag aagtcttcct
     3661 atattaattg aatttgcaga agataatata taattattat ccaatagttt ttgattaaat
     3721 tttttatcag aaaaaatctt ctttacacct gattgtgcat catcaaaatt tccttttata
     3781 ccaactacat atgtattttt tccagtttga gttttcattt gaagtttttg tactgggctt
     3841 acaccatctt ctgggaagaa tacaattatc tttattttat ctatatcttt aaatccctca
     3901 agtgcagctt tgcctgtatc tccagatgta gcagtaagaa tcacaacatc tttctctaaa
     3961 ttatctttct ttatagatgt tttaagaaga tgaggcatta tagtaagtgc catatcttta
     4021 aatgctaaag ttggaccgtg atatagttct aagaaataag tatcatttac tttatttaat
     4081 ggagctatac tactacaatc aaacttttca tcatatgcat tttctataca atttttaatt
     4141 tcatcttcag taaaatcaca taaaaattta ctaagtacaa aaaatgctat ctgagaatac
     4201 gttaaattta ctaaatttat aagttcattt tttacattgg gaaattcact tggaacataa
     4261 agaccaccat cacttgatat tccttttatt attgcttgtg atgcagttac tttttcttcg
     4321 gtgcctctcg tacttgaata atacattttt tataatcctc catatatttt aaatttttaa
     4381 ttagctaatt aaattagata cagaatattt taaaaaccga atttaggtaa taaaaaaaga
     4441 ccctcatccc taaaaaaggg acgagaatct gtttcccgcg gttccaccca aattgatttt
     4501 aaaaaatcca cttgatactt taacgcaagt aataaaaacg tctattccta ctaaaatcat
     4561 taagataact tcggatagaa gctcctaggt agttttcaat ccttctaatt agggaaacat
     4621 ttcagctagt agtttccttc tctggtaatc gattcagatt tactcttcct aatcaatgcc
     4681 tttcaatatt cttaatatta ttaattattc tagtttataa aacagacaaa gtcaagtatt
     4741 aatataaaaa tttggaaaaa atatttttta aattgtaaaa aaattgtaat caaattgagt
     4801 attagaaaaa tattttttta acttttttaa aagtttaaaa acctagattt tcatagtgat
     4861 gaagctgttt ttaatgaaag taatcaaaag ataactatta aatatctgta atattacaat
     4921 tttatactac actatattac aaaaaaagtt tacaaaactt taatttgaaa aatgtaatta
     4981 tatggtatat catataaata taagtaaata gtgctaatat ttaaactatt aatcataatt
     5041 ggttcaacaa aagtatatag ttaataatgt ttaatttgaa gaaattgtta gtgtgaaatt
     5101 acatatatat tcaaattaaa ttcataaata tttcacataa taatagtata ctgtaaacaa
     5161 aaggaggtat taaatatggg aatagtactt ataacagcag cattagtttt ggttatgtat
     5221 ggactttcaa ttctatcata tatatcagaa agcaatatat tagaagacac tgtaaatgat
     5281 aaccaataag gttttgaatt atataaataa gtgagaactg taaataaaag ttaattttta
     5341 tttgcagttt ttttatatat atgtaaaata aagttaatta ataaaaaata atgttttgtt
     5401 tgtagtatat atggataata atagtaaagt ataagaatga tgagaataat atataagaaa
     5461 taataatata aagagaggta aacattatgg tagatatcat tgtaattggt gctggaccag
     5521 caggtttaac ttcagcaatt tatgctatga gagcagggtt aagcgttaca gtatttgaaa
     5581 aaaatattta tggaggtcaa gttgcaagta cctctgaggt agaaaattat ccagcagttc
     5641 aaaagatttc tggggtagaa ttttctaata atatatacaa tcaagcagtt gctcaaggtg
     5701 ttgatattca gtttgatgag gttgaagaga taaatttaga gggaaaagta aaggttgtaa
     5761 aaacttcttc aggtgaacat aaagcaaagg ctgttattct tgcaaatgga gttgaaagac
     5821 gtaaattagg ttgtgcaggg gaacaagaat ttacaggtag aggagtatct tactgtgcaa
     5881 catgtgatgg agcctttttt aaagataaag aagttgctat agtaggtgga ggaaatactg
     5941 cactagaaga tgctttattt ttagctaata attgtacaaa agtgtattta attcacagaa
     6001 gagatagttt tagaggtgaa gaagtgttgg agaaatctgt gaaagcaaga gagaatattg
     6061 agatacttta cagtcatggt gtagaaaaaa tagaaggaga aaagactgta tcgaaaatag
     6121 aagtaaaaaa cttaaagact gaagaaaaaa gaactataga tgttagtggt atttttattg
     6181 ctataggtct taaacctaat aataaaatgt ttgaaaatgt tcttgatttg gatgaaggtg
     6241 gatatataat ttcagatgag tcttgtacaa catcagtaga aggtgtatat gtagcaggag
     6301 atagtagaac taagttttta cgtcaaataa taacagcagc atcagatgga gctattgcag
     6361 ccgttcaagc agctaattat ataaatgttg aataatataa atttcaaata aaaatagcca
     6421 tataaactga aataattttt cagtactata tggctatttt gttaattaga ggtaaatgta
     6481 cgttttattt accttgtcta gatttgttat atcttaatct atcatgttca tttaagaatc
     6541 tttttctaag tcttatagaa tcaggtgtaa tttcaactaa ttcatcatct tctataaact
     6601 ctagtgcttc ttcaagagta aatattctag gaggagataa ttttatagca tcatctgaac
     6661 cagaagcacg tacgttactc attttcttgt ttttacaagg gttgactacc atatcatctt
     6721 ttctggaatt cataccaatt atcatacctt cgtaaacttc tacaccaggg tctacaaaca
     6781 ttacagctct atcacttaag gcattaagag aataccccat agtaacacca ggtccttgtg
     6841 ctattaaaac accatttcct cttgaaggta tttctccttt aaattcttca aatttttcaa
     6901 atgaacgaac taaagtacct tctccacgag tagcgtttat aaattcactt cgatatccta
     6961 aaagaccacg agtaggtgct aagaactcta tttttacata gtctccttct attgacatag
     7021 actccatcat accttttctc atgtttaatt catttataat agtaccagag taaacttcag
     7081 gacagtttac aacaactctt tctattggtt ccattagttt tccatcttct ttatgcatta
     7141 aaacttcagg tttagaaaca ccaacttcat aaccttcacg tctcatattt tcaagtagta
     7201 tagataagtg aagctctcca cgtccagaaa ctttgtatcc atcagtagta tcaagaggtt
     7261 caactttaag accaacattt acttcaagtt ccttttcaag tctatctttc aaatgtctag
     7321 ttgtaacgaa ttttccactt tttccaacga atggagagtc gtttactaag aagttcatag
     7381 aaagagtagg ctcttctatt tttatcattt ccataggtag tggactatct aaatcgcata
     7441 tagtttcacc tatagatata tcaggaatac cagctataac aactatttct ccactagtag
     7501 cttcatcaac ttcaacttgt tttaaacctt catacacaga aagttttgat acttttcctt
     7561 tagatacaac agaatcttct ctacatatag caacttgtgt attgttttta agagtacctt
     7621 tgtaaattct acctatacca agtctaccta cataatcatc atatgcaaga gctgatattt
     7681 ggaattgtag aggttcatta tcgtaattag gataagcttc aacgtgattt acaatagttt
     7741 taaatagagg agaaaggtct tcactatcat catccatttc aagtttagca ataccttgtt
     7801 tagcaatacc atatattata ggaaattcgc attgctcatc tgttgcattt aagtctacaa
     7861 ataaatcgaa aacttcatta actacttctt ccgctctctg gtctttttta tcaatcttgt
     7921 ttataaataa tattggtttt aaaccgaact ctaaagattt ttgtaaaacg aatctagttt
     7981 gaggcattgg gccttcacta gcatcaacta atagaataac tgtgtcaaca gttttcataa
     8041 cacgttcaac ttcagaagaa aagtcactgt gtcctggagt atcaactatg tttattttgt
     8101 aatcttcata gtttattgca cagttttttg aatatatagt tatgcctctt tctttttcaa
     8161 ggtcgttact gtccataacg cagtctttaa ctacttcgtt ctttctaaaa acaccgcttt
     8221 gagaaaggaa agcatcaacc aatgttgatt ttccagcgtc aacgtgggct ataactgcga
     8281 tatttataat tttatgtttt tgtgacattt ttgttgctcc cttcgattaa aataacttat
     8341 taattatagc acaatatatt attaggtaca aggaggaata tattaaattg tattaatata
     8401 gtttaaattg gataaaactg ttagttttgc atctgaaaat tattctgttt aaaaaaattt
     8461 tttagacaaa taataatctt aatagataaa aagattaata aaagagtaaa ggatgatata
     8521 tgttggaagt aaccaaaata ataattaaat attttgcaag tgaaagtgat acttttataa
     8581 aagaagaagt ttgtaatttt gaagaacttc ttaagtggtc tcataaattt attgaaggta
     8641 aaaagataga agatgttttg tatataaagt ctaaagaaaa ctataaagta aatgtgatta
     8701 agtctttaga aattaaggaa ttggaaatag tgagaaaaca acagatacca ccgtacatgt
     8761 agtattaata atatataaaa ttaatatgta atactcacaa taggatataa atggtatata
     8821 tggtaataat ttatattaaa aaaactttta taaagtttat atttgggtat aaaagtatta
     8881 attagacatt aattaggagg aacttaaata tgataagagt atgtccaaac tgttcaaatg
     8941 tggatgtaaa taagctaaaa actttggttg gagaagaaaa tgtaaagact ggttgtatag
     9001 gtcagtgtag agcatttaaa aaagaagctg ttggaataat agacaatgag cttgaaataa
     9061 aggaaacaga agcagagttt tttgaagcat gtaaagaata aattttaaat aaaatataaa
     9121 ttataataaa tagagcatct aaattgtata agcattaaac aatttagatg ctctattttt
     9181 atatatacat ttaattatga gtatactttt ctattaaata caaataaata taactaatac
     9241 atttatttat taaaaaaata ttgatttaat atacctatag ggggtataat aaaaatatag
     9301 atttctaaat aagtaattta tcaatattac atatcaaaat aaaacgttac aaattagctg
     9361 aaaatatatt gaaagaaagg aagattttat tatgagttca aataaagtgg tagaaagtta
     9421 taaaattaca ggtatgacat gtgcagcttg tgcaaaggca gtagaaagag taactaagaa
     9481 gatggatgga gtatatgacc aaagtgtaaa tatagctact gaaaaactta aaatagaata
     9541 tgataattct aaggttaatt ttgatgatat aaagcaggtt gtagaaaaag caggatatgg
     9601 aattataaag gaagaaagta ataaaaaaat tgatatgaaa attgatggaa tgacatgtgc
     9661 agcttgtgca aaagctgtag agagagtggt aaaaaaattg gatggagtag aaagtattag
     9721 tgtgaatatt gcaacagata aagctaatat agactatgac ccatcaaaag taaaattatc
     9781 tcaaataaaa gcagctattg aaaaagctgg atataaacca atagaagagg taagaaataa
     9841 ggttgatgtg gatgaagata aattgagaaa agaaagagaa atgaagtctt tatttgttaa
     9901 atttatagtt gcaatagtat ttgcagtacc tctgttttat atagcaatgg gacctatgat
     9961 aataaagcct atagggccat ggcctttgcc agaaattata aatcctatga caaatacttt
    10021 taattatgca ttaattcagt taattttagt aataccagta atgatagctg gttataaatt
    10081 ttatataaat gggtttaaat cacttttttc tttaagccct aatatggatt ctttagttgc
    10141 aataggaaca ttagccgcat ttttatatag tctatatacg acactacaaa tagccaatgg
    10201 acaaattcaa ggtatgcacc atcatcagtt gtattatgaa agtgcaggaa taataatagc
    10261 acttatctta cttggtaaat atttagaatc aaaatcaaaa ggaaaaacat cagaagcaat
    10321 taaaaagctt atggggctac aacctaaaac tgcaatagtg ttagttgatg gtaaagaagt
    10381 tgaaactcct atagaagaag tagagatagg agatatacta ttagtaaaac caggaactaa
    10441 aatacccgtg gatggagttg taatagaagg atatacatca gttgatgaat caatgcttac
    10501 aggtgaaagt ataccagtag aaaaaaatgt tggaagtaag gtgacaggag ctagtattaa
    10561 taaaaatggt gttattaagt ttaaggctga aaaaattggt ggagatactg ctcttgccca
    10621 aataataaag ttagttgaag atgcacaagg tacaaaagca cctattgcta aacttgccga
    10681 tactgtatct ggatattttg taccaatagt aattgcaata gctgtagttg catcattatt
    10741 gtggttttta ataggaggaa aagatatagt atttgtactt actatattta tatcagtact
    10801 tgtaattgct tgtccttgtg cattaggatt agcaacacca actgctataa tggttgggac
    10861 tggaaagggc gcagaaaatg gaatcttgat aaaaggtgga gaggcactag aatctgcaca
    10921 taaagtaaat acagtgatat ttgataagac aggtactata acagaaggaa agccaaaagt
    10981 aacagatata gtattaaata ataatgtaaa agaagaatat cttatcaaga ttgcttcaag
    11041 tgcagaaaaa ggttcagagc atcctcttgg agaagcaata gttaagtatg gagaagagaa
    11101 gaatataaaa tttgaaaaag ttgataattt taaagccata ccaggtgctg gtatacaagt
    11161 tactattaat gatgaaagta tattacttgg aaatagaaag cttatgaatg acaataatat
    11221 aaagcttggg gatttagaag aaaaatcaaa tgtattggca agccaaggta aaacacctat
    11281 gtatatagct gtagatggaa atttatcagg tataatagca gttgcagatg tagtcaaaga
    11341 aagtagtaaa aaagcaatag aaatacttca tgatatggga ataaaagttg caatggtaac
    11401 tggagataat gcgaaaactg caaatgctat agcaaatcaa gtaggtatag atatggtttt
    11461 ggcagaggtt ttaccagaag ataagtcaaa agaagttgaa aaactacaaa atcaaggaaa
    11521 gtttgttgca atggtagggg atggaataaa tgatgcccca gctcttgcaa aagcagatat
    11581 agggatagca ataggaagtg gaacggatgt agccattgaa tctgcggata tagtacttat
    11641 gaagagtgat ttaattgatg ttccaacagc aattaaactt agtcatgaaa ctataaaaaa
    11701 tataaaacaa aacctatttt gggcatttgg atataatacg attggtatac ctgtagcagc
    11761 aggaatatta tatgtatttg gaggaccttt attaaaccca atgattgcag cagcagcaat
    11821 gagcttaagt tcagtttcag tcgtatcaaa tgcacttaga cttaaaaact tcaaggcata
    11881 taagagagat tagattaata aattaaataa aatgtatcca aaagagctat ctttaaatta
    11941 aaaggattaa tttacaaatt aagatagctc ttttttcatg taaaattcac aacaataaga
    12001 taaaatataa gtaatatacg aaattttaaa taaaatagaa aggattgata gaatgaattc
    12061 gtcaatactt gtaattgaag atgattcaaa tatacaagag ttaatatcag aatttttaag
    12121 tgcagaaggt tatcaagttg atacagcaaa tgatgggttg gaaggtatac aaaagtttaa
    12181 gcaaggaagt tatgatttag taatattaga tataatgatg ccaaacttag atggttatgg
    12241 agtttgtaaa atgataagaa agtcatcaag cgttccaatt atatttttaa ctgctttaaa
    12301 tgatgaagga gaccaactta aagggtttga cttagaatgt gatgattata taacaaaacc
    12361 attttcattt aatcttctta taaaaagagt agaagcaatt ttaaggcgaa gtaacaagac
    12421 tataaatgac aaatttatag tttttgaaaa attaaaatta gatttaaaca catatattgc
    12481 agagatagat ggagaaccta tagaacttac actaaaagag tttaatatat taaaagcttt
    12541 gatagaaaag tatccacaag ttataactag ggaaggtctt ttggatagta tatggggtta
    12601 tgactattat ggagatacta ggattgttga tgcacatata aaaaatataa gaaaaaaaat
    12661 atcgttacca tatattaaaa ctgtaaaagg aataggatat acacttgaga aggatatttg
    12721 ataagtggga aaagttaagt ataaagtata agttattttc aataacgaca tcattattaa
    12781 tagcgttagc tttaataata tatctaattt tatatttttt attgccatca tattatcatg
    12841 agtataaaat tgagtctctt caagaaagtt taaaatcttt agtagatagt tcaattcatt
    12901 tcgatacata tactttagaa gaaagacttt attatatggc taaagaccaa aatttggcta
    12961 ttttattaaa agataaccaa gggaaaattg tatatggtaa gaatgaagta gttattttaa
    13021 gatatagtaa gtacatgatt aatagtcttg aggatgagta tagaacttct atacctatat
    13081 atactaaaga tgcaaaagat ggtccatata ctttagaact cgttatgcca ttacaaccaa
    13141 ttgacgaagc aaacgaagtt ataagaaaac taatgccata catcatatca atagcaatat
    13201 tgatagcgat tattggagca tatatatatt caattgtaat aacaaagcct cttataaata
    13261 taatagagag tgagagagaa caggaatata gaaggaagga ttttgttgct acaatatcac
    13321 atgaactaaa aactccaata accataataa gtggtcaaat tgaaggtatg atttatagtg
    13381 taggtaagta taaggatagg gatacttatc tgaaaaaatc atatgaatgt acacaagaat
    13441 taaaagattt agtaaatgaa atgatagagg tttcaaaatc agaaatactt gaaaaagatt
    13501 tgaaattggt atcaataaat ataagtgaat tattaaatag acttgtaaag agacaagtat
    13561 ttttaataga agaaaaacat atgaaaacta ttttaaaaat agaagaaaat ttggaaatta
    13621 aagcagacca agagagaata actaaggcaa tcaataatat aataaataat gccataaaat
    13681 attctcctga aaaatcagag cttataatta gactctatga taaaaataaa cgaataagta
    13741 aaaagacttc taatcaaaga gtggttttag aaattgaaaa tactggtgta actatcgaga
    13801 aacgttattt agaagagata tttaatccat tttatagaat agaaaaatcc cgttcaagaa
    13861 aaactggagg aagtggactt ggactttata tagtcagtca aatttttaaa agtcatgggt
    13921 ttgattatag cataaaaaac aaaga
//
LOCUS       DACTGW010000092        13720 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.092, whole genome shotgun sequence.
ACCESSION   DACTGW010000092 DACTGW010000000
VERSION     DACTGW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.092"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            51..647
                     /locus_tag="I9573_002063"
     CDS             51..647
                     /locus_tag="I9573_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HAT4796683.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVNKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(745..1704)
                     /locus_tag="I9573_002064"
     CDS             complement(745..1704)
                     /locus_tag="I9573_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAT4796684.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(1738..2469)
                     /locus_tag="I9573_002065"
     CDS             complement(1738..2469)
                     /locus_tag="I9573_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796685.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(2462..3232)
                     /locus_tag="I9573_002066"
     CDS             complement(2462..3232)
                     /locus_tag="I9573_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4796686.1"
                     /translation="MFEKIHKRFKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(3317..4027)
                     /locus_tag="I9573_002067"
     CDS             complement(3317..4027)
                     /locus_tag="I9573_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HAT4796687.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(4207..4359)
                     /locus_tag="I9573_002068"
     CDS             complement(4207..4359)
                     /locus_tag="I9573_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAT4796688.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(4385..6541)
                     /gene="feoB"
                     /locus_tag="I9573_002069"
     CDS             complement(4385..6541)
                     /gene="feoB"
                     /locus_tag="I9573_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAT4796689.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTIIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHVPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(6641..6862)
                     /locus_tag="I9573_002070"
     CDS             complement(6641..6862)
                     /locus_tag="I9573_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAT4796690.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(6875..7084)
                     /locus_tag="I9573_002071"
     CDS             complement(6875..7084)
                     /locus_tag="I9573_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAT4796691.1"
                     /translation="MPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSKI
                     DGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(7611..8219)
                     /locus_tag="I9573_002072"
     CDS             complement(7611..8219)
                     /locus_tag="I9573_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT4796692.1"
                     /translation="MVDNLIKYIHKSNIAIVVLHEIYGVNNFIKDVCQKYYKGGYDVF
                     CPELLGEKRVFSYLKAQEAYDFFINNVKFDIYKKVEKLINGLKSEYKHVFVVGYSIGA
                     TIAWRCSENSLCDGIICCYGSRIRDYMDVIPTCPILLVFAKYDSFDVDYVSSQLSRRQ
                     NIQIEILDANHGFIDTYSKNYSNAQTEVFNIKEKNFLSKCLG"
     gene            complement(8339..8767)
                     /locus_tag="I9573_002073"
     CDS             complement(8339..8767)
                     /locus_tag="I9573_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAT4796693.1"
                     /translation="MYGVIGATVLVIFVILTAGIMQGKKVDFQLPTVFMIVTFIVSAI
                     SEEIVFRGYIQTRLTGLIKNSVLSSCINAFLFLSTHYPVKWISSGDFSFAILSGFYVI
                     CLILLNFSCDLVYRKTNCLWGAWLLHILYNIGTGVLIFTT"
     gene            complement(8786..9046)
                     /locus_tag="I9573_002074"
     CDS             complement(8786..9046)
                     /locus_tag="I9573_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796694.1"
                     /translation="MFKTVFEDKEYVLLCKNYSKSDGVKAIFIYLISMLLLFFQGYLY
                     TTNLSSTILTNSQMILSILILLIGLGFVLLSKEKLNTIGITM"
     gene            complement(9320..9865)
                     /locus_tag="I9573_002075"
     CDS             complement(9320..9865)
                     /locus_tag="I9573_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HAT4796695.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(9983..11806)
                     /locus_tag="I9573_002076"
     CDS             complement(9983..11806)
                     /locus_tag="I9573_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796696.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(11819..13594)
                     /locus_tag="I9573_002077"
     CDS             complement(11819..13594)
                     /locus_tag="I9573_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796697.1"
                     /translation="MKYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLREKIGVVFQNSSVFGKSILENIVMGNEGISREDVIKSC
                     EIANANQFITKLENSYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         4615 a         2464 c         1361 g         5280 t
ORIGIN      
        1 tataatctaa ttacttgata ttaattttca tttgaaggag gttatctttt atgtcttgtt
       61 gtaattcaca attttgttgt aaaaataaaa ttgaatcatc atatataaaa tggcttttag
      121 aggttttagg tccagtcttt ttaggctcta aacctgctga aataataaat atttcgttta
      181 ctgatgtaaa tagagaggaa aaaattaatg atatttataa atatctatat aaatgtaaga
      241 aaatagattt tatagttatt gataaagaaa aaaagggatt aaaaatctta tttgtaaata
      301 aaaaagcatt atcagaaaaa ttaaaatgta aaaaaacagt aaacttttta aagtttttag
      361 gatacaaaca aaatacgaat gttaatgctt atttagagca tttagtaaat aaacttaaga
      421 gtgatgtttt tcctgatgaa ataggtattt ttttaggata tcctcttaaa gatgtgattg
      481 gatttatggg atatagtaat tatgaagttt ctatgattaa atattggaaa gtttatggtg
      541 acacaaaaca atccgaggac acatattcaa agtttttact tcaccgtaag aaaatgagaa
      601 aattactaga ctacataagt gtagataaaa tagtaagttg tttttaattg tatttttgta
      661 taataataaa aatacaccct cttttaccat acagtttcca taataaataa gggtgtattt
      721 tccactttaa tattttaaag tattctaatt ttgattgtac aacatatctt taatatttat
      781 tttcttttgt tgcattccat tagacatcaa aaattcctgt gtatcttcta attcttttat
      841 atccttgtcc gttattttag aactaaagtc ataccaagaa tacatttctt ttgtttcatc
      901 taaagataaa ccaacttctt tagatacaac atccatcact tcatccttgt tttcattcat
      961 atattttaaa gtttcttcat gaaccttcat aaatcttttt actaattcag gatatttttc
     1021 agcaaagtca tcacttacag cagtaactat tattccactt actaatcctt ctccatttac
     1081 aatcattttg gcacctgaat ttatcgtttt caatgccact ggacctgcaa gcattgctac
     1141 atcagcactt ttactttcta atgcagaagc agcactagga atatccatat ttacatattc
     1201 aacatcatct atagtatagc cttctttagc aagtgcagca atcaacagtt gatgaagtat
     1261 agtccctttt ggacctacaa tttttttacc ttttaagtct tttatagatt taatattatc
     1321 attattagtt ataagcatat atccttttgg tgaacggcta taaatattcg taattttaag
     1381 accaacacca tttgatgcag ctattattgc agacgttcct cctaatgcat gtaaaaagtc
     1441 tagctcacct gctgctaatg cttgagtttg ctctggtcct gttgtcaatt catggaagtt
     1501 gacttttata ttgtcctttt taaattcctt tccaaataaa tcatcctttt tttctaatat
     1561 actaggaaca ttaagtggtg attttacata agttaaattt atttccttag gtaaactagc
     1621 ctcttttttt gcaggttcat ctttcttatc atttgttgtt gaacatccta cagtcccaac
     1681 caacaccatt attaaagatg tcattactgc taaaatcttt ttgtttcttt taatcaatta
     1741 aaactccccc aattccctta atatttcttt tttcaagttt atataatagt ttttagttaa
     1801 atctctatta tattcatctt cgacactaaa ttgtttaatc cttttatttt tactaaaaac
     1861 aattattttc tttgctattt ccatagcttc ttcaatattg tgagttacaa atactacacc
     1921 ttttttagta tttttatgga tatttacaac ttctttttgc atttttcttc ttgtgaagta
     1981 atctagcgct gcaaatggtt cgtccatcag caaaatatcg ggattgaagg aaagtgctct
     2041 tgctatactt actctgtgag ccattcctcc tgataattca tttggatatg agtttttaaa
     2101 cttttcaagc ttcatcattt ttaaatactt gtctaaatca actttatttc tattatcttt
     2161 ttcagtatgt aaaagtatat tttcacttac attcagccat ggcataagtc tactttcttg
     2221 aaataccatt cctacttttg gagtacactt tttatcatta ttaaaaaaat atatgtttcc
     2281 actagtagca ttttcaagcc ctgctaaaat tctaagtagc gttgttttcc cacatccact
     2341 ttcacctaat ataacagtta tctcttcact agatatattt aaagatatat tatcaagaac
     2401 taaatgctct ttattatcaa ctaaatattt cttatatata ttctctagca aaaatccact
     2461 tctaaccatt taaatcaacc tgcttccctt tgctaaattt tgttattatc tttgaaaata
     2521 gataatctga aagtatacct aaaaatccaa taacaaaaat tcctaccaat acaacatcag
     2581 ttctagacat atcttttcca tcagaaatca aatatcctag tcctgaagaa gctgcaataa
     2641 gctctgctcc aattatagcc ctaaaactat acccaagccc taaactaagt ccaacaacaa
     2701 tatccagcat agaattaggg attattattt tataaaaaat tctcttttta gataaattaa
     2761 aagatttacc tacttctatt aatttattgt cacaattttt tatacctttc aatgtattta
     2821 aaaatattgg aaaaaaggat gccaaaataa taattataat tttagattct tcacctatac
     2881 caaaccataa aataagcatt ggcaccaaag ctagtggagg tgtacttcgc ataaattcaa
     2941 atagtggttt gaaatattca tacatttttt tatttactcc aaatacaact ccaagtggta
     3001 tagccatcaa tgaacttatt aaaaaaccta ttaatatcct tcttacactg gcataaacat
     3061 ttaagaaaat agaaccgtca tatatcattt ttataaaagt atctaagact atttcaggag
     3121 gaggcagaat atagctactc catataccca aaacacatac tatcttccat aaaattaaaa
     3181 ttattaaaaa aatataatat cctttaaacc ttttatgaat tttctcaaac atataaaact
     3241 ccttttaaca tatctatccc tgctaccctt tttatttttt aatatcttaa ataataatta
     3301 agctatatca taagatttaa tctatgccaa gtcttaatcc acctgaactc attctatcag
     3361 aaggtgtaga atctgttttt aagccagaca atctttcaga tggttgtata atgactattt
     3421 gtccaggttc aacaaattcc atatgataag attctcctga agtctgacca ataaaagtct
     3481 tccatgatag ttttgatact ttaggatatg gttctcttcc agtccaagct actaatgcat
     3541 ctgggtcaac acaacaaggt ccttcaagaa ttagaggatt accatctgtt attatagcaa
     3601 catcttgatt ttgacctgtt ttatttacta aatgtgtagt aaaaagacct ttttgagata
     3661 ttacccctga accaatcaat tttacactgt aatttaattg ttcagaaaat gccaataagt
     3721 tttcactctc aacataaatg gaatcacctg ggtcaattga tattacatct acatggtaag
     3781 cattttgagc taggtaaatt tcaccttcac cttcaaccac cattattggc atttgttctc
     3841 ctgtcaatga cctctgaaca tgacctaata atgctcttcc caatccaccg ccattacttg
     3901 gacctaacag caatttttca aaattaaaat ttcctttaaa ggctaccata gcaccttttt
     3961 ttgcaaaaaa tttaccactt ccccttgcta tacacataag ctcgtttgta acatcaaatt
     4021 taaacataat attcacttat attgcttaat ttagcaataa tccctcctaa tcatatattt
     4081 atatataatt atacagtaaa attacagcat agtctaacct acttcaaata tgaaatttgt
     4141 tatttatata aaaaataaga acctgataat aaagcaattt aactttttat caggctctta
     4201 tcctatttat ctcttagcgc tatgacaact cttactactt gagcatccag aacaaccaca
     4261 tccacaactg ctaccattct tttttgaatt tttaaccata tacattacag ctagtgccat
     4321 taatactact acaatagctg ctataataaa tgtagccata ttcatcactc cttatttata
     4381 tgaattaatt tttcatataa cttaatttac ccattttttc ttttttcata tttttatttg
     4441 aataagttaa aactacaaag actactgcta ctgctataaa tatagatatt attgccccag
     4501 caatacttcc tccagtacct aaaactaaac ttccaacttg atttataaga agagcaatta
     4561 tatacgctgt taaagtttgg aatcctaatg taatccatgt ccatttccat gaacccattt
     4621 ctctttttat ggctccaata gctgcaaaac aaggtgcaca aagcatatta aatgccataa
     4681 atgcaaacgc actagctact gtaaacatac ttgcaactga agcaagaagt gttgggtctt
     4741 gctctgttgc atctgatata ccaaataaaa ctccaaatgt tccaactaca ttttccttag
     4801 ctactagacc tgtaactgtt gctactgatg attgccaatt tccaaatcca agaggagcaa
     4861 atattggtgc aactatattt cctaagctag ctaatatact atctccagca tctaccattt
     4921 gtaatgacca attaaatgat tgtaagaacc agattactcc acaagcaaca aatataattg
     4981 ttcctgcttt gattataaat gcttttcctc tatcccacat atgtattaaa acaccttttg
     5041 cacttggaac atgatattga ggtaattcca taacaaatgg agatggttct cctgcaaata
     5101 aactagtttt ctttaatata attccacaaa tgattatcat tgcaatacct aagaaataca
     5161 ttgatggagc tacccatgaa gcacctccaa ataatgcccc tgcaaataat gctattattg
     5221 gaatcttagc tccacatgga atgaatgtag ttaacataat agtcattttt ctatctctat
     5281 cattctctat agtacgagtt gacataactc ctggaactcc acaacctgaa cttataagca
     5341 ttggtataaa tgattttcct gaaagaccaa acttacggaa aattctatcc attataaaag
     5401 ctacacgtga catataccca caatcttcta atattgacaa taataagaat aaaagcatga
     5461 tttgtggtac aaatcctagt actgctccaa ctccaccaat tagaccatca actacaagtc
     5521 cttgtaacca ctctgctaca tttaaagaag ctagaaaatt acttacattg ccttgaataa
     5581 tttctccaaa cagtacatca tttgtccaat cagttgctat agtacccaat gaacttactg
     5641 ctatatagta aactccccac attataagtg caaaaatagg taatgctagt atacgatttg
     5701 ttacaatctt atctatctta tctgatacag tttccttacc ttttcgattc ttctttatta
     5761 tacttgaaat tattgaacta ataaattcat aacgattggc tgttattata ctttcactat
     5821 catcatccaa ttcatcttca caatttctag ttatttcttc aatactattc aatattgttt
     5881 tagaaatatc aagtttttgt ataacatttt catctctctc aaatagcttt atagctaacc
     5941 agcgtgtttc tacatctata tatggagtct tttcttctat tattttttca attttttcta
     6001 ttgcgtcctt actttcatca acaaaaggta atttgaaatt taatttattg ttactacttg
     6061 caagttcaac tgccttttca gcagcctcca taattccttg acctttaact gctgttactt
     6121 ctaccactgg gcaacctata acttcagata gttctttaat atttatctta tcaccattct
     6181 tatttacaat atccatcata ttaagagcta taatggtcgg tatacctagc tctaaaactt
     6241 gagttgtaag atataaattt ctctctatat ttgatgcgtc tacaatattt attactgcat
     6301 ccggcttatc atctagcata aaattacgtg taactacttc ttctaatgta tatggagata
     6361 aagaataaat acctggtaaa tctactattt ccacatcttt atttcctttt aattttccac
     6421 cttttttttc aactgttact cctggccagt taccaacata ttgcgacgag cctgtaagcc
     6481 cattgaacat tgttgtctta ccacagttag gatttcctat gagacctatt tttattgaca
     6541 tttccattcc ctccattatt atttaaatac atcactgttt gtgatacaga aaatcattct
     6601 cattatataa ttacattaac cctactctaa ttaatcttta ttattccaca ataatttttt
     6661 ctgcatctga cttacgaatt gatagttcat aatctctgac tgttacctca attggatcac
     6721 caagaggagc tacttttctt ataaatatat taacacctct tgtaatcccc atatccatta
     6781 tacgtcttcg tgtagctcct tctccttcta actttttgac ttttacagtc tctccacact
     6841 taatatcctt cagtctgttc atgtcattac ctccctatat tattattcta cttgccattt
     6901 ttttatctaa tgcaactcta gtatctttta cgtctattat taagttacca tctatcttag
     6961 aaactatttt aacatttgta cctactacaa aacctatact atttaaaaaa gtcctcgtct
     7021 tatcacttcc cgaaattttc tttataacta cttcttcacc tgtatttgat aatatcaatg
     7081 gcatcatata atattcctcc tcttcaacca atttaaattt acaaacaacc tctatcttat
     7141 atataaatca caatttctta aattttgact cgttaaaata attacttatc tttttcctta
     7201 ttcttacaat tcctaatttt gatttaagtc tatcacaata gcatattttt tgcaagtgat
     7261 tattgtattc ttttgatttt ttttctcatt tgatttattg tattaataaa atctataaaa
     7321 attcatactg ctatcctata tattttgaga tttctcttag atactcataa ccatattctg
     7381 cataagaatg ttcttttgat aaagatatta ttctatagta atgatttttt tcttttcaat
     7441 gtaatttaaa tattaatcta atacaatcat aaaaacataa ttaattctat gattctactg
     7501 actatctcta tctcaaaaaa tataatgaca ctactaatat tttttaacat tagtagtgtc
     7561 attatgtttt tcaaattgat taaattttct atattttata ataaaacata ttagcctaaa
     7621 catttagata gaaagttttt ttctttaata ttaaaaactt ctgtttgagc attagaatag
     7681 ttttttgagt atgtatcaat aaaaccatga tttgcgtcta aaatttcaat ttgaatattt
     7741 tgccttctag aaagttgaga agaaacataa tcaacatcga atgaatcgta ttttgcaaac
     7801 actagcaaaa taggacatgt tggaattaca tccatataat ctctaattcg ggagccataa
     7861 caacaaatta taccatcaca caaagagttt tcactacatc tccatgcaat agttgcacct
     7921 atactatacc caactacaaa tacatgttta tactccgatt ttaaaccatt tataagtttt
     7981 tctacttttt tataaatatc aaacttaaca ttatttataa aaaaatcata tgcttcttga
     8041 gcttttaagt atgaaaaaac tcttttttct cctaagagtt ctggacaaaa cacatcatac
     8101 ccacctttat aatacttttg acaaacatct tttataaaat tattaactcc ataaatttca
     8161 tgcagtacaa caatagctat attactttta tgtatatact taatcagatt gtccaccacc
     8221 tgtcgatatt ttttacatct agtttaaatt attttaagta tgtcaattta ctagctctaa
     8281 aatttgactt ataaaataat ccgtttctta cgataaactt tctgcaaagt aaaattgact
     8341 aagtagtaaa aatcagcact cctgtgccaa tgttatacaa gatatgcagt agccatgccc
     8401 cccacagaca gttagttttt cgatatacta aatcacatga aaagttaagt aatataaggc
     8461 atatcacata aaatccagat agaattgcaa atgaaaaatc tccacttgaa atccatttta
     8521 cagggtaatg tgtagataga aaaagaaaag cattaataca agatgaaagt acactatttt
     8581 ttattaatcc agttaacctt gtttgaatat accctctaaa aacaatttct tctgaaatag
     8641 cagaaacaat aaatgtaaca atcataaaaa cagtaggtaa ttgaaaatca actttttttc
     8701 cttgcataat tccagctgtt aaaataacga aaattacgag tacagtagca ccaataactc
     8761 catacaaaag acttttattt aaatttcaca ttgtaatccc aatcgtattt agtttttcct
     8821 tcgaaagtag tacaaatccc agccctataa gtaaaatcaa gattgacaat atcatttgtg
     8881 agttagttaa aattgttgaa cttaaatttg ttgtatacaa ataaccttgg aaaaacaata
     8941 gtaacataga tatcaaataa ataaatattg cttttactcc atcactttta gaataattct
     9001 tacataataa aacatattct ttatcttcaa atacagtttt aaacactata aatactcctt
     9061 tctaattaaa ttcttattta tttctcatat tttatcataa atatatgttt aaatcaaatt
     9121 aattctattt attacataaa tcagagataa taaaactaaa aaattaattt cattaaaact
     9181 atatttataa tgatttaaat atttacaaga ttatagctat aaaaaatata aaatacaact
     9241 attaaaaagg agcatatcat tataatttta aaaatcataa atgatatact cctaaaaatc
     9301 tacttaactt atattaaatt tattttccaa aatatctatt taataatcct aagaatgctt
     9361 taccatgtct agcttcatct ttacacattt catgtactgt atcgtgtata gcatctagtc
     9421 ctaattcttt agctttttta gctaatttta attttccatc agttgcacca tactcagcat
     9481 caactctaac tcttaagttt tcttttgtat ctgcaactac aacttctcca agaagttctg
     9541 caaatttagc agcatgttca gcttcttcaa gagctattct cttataagct tctcctactt
     9601 ctggataacc ttctctatca gcttgtctac tcattgctaa atacattcct acttctgtac
     9661 actcaccaac aaagttagct cttaatcctt cgattatttc ttcatctaca ccttgagcta
     9721 ctcctattct gtgctcatca gcccaatttt tttcgccttt catttcttca aatttatcag
     9781 ctccaacttt acatactgga cattgtgctg gtgcagcatc tccttcatgt atatatccac
     9841 atactgtaca aacaaatttc ttcataatta ctttcctcct aaattttata tatcaaatta
     9901 aattttttat ttattaagtt agtctatata tacccgcacc atataccttt aaacaatttt
     9961 tttaaaagtt ttttctaaat acttattttc cctgtgtcaa taatatgttt ctataataat
    10021 gtccctttaa ttttatcaat tctttatgat ttcctgattc cacaattcta ccattttcta
    10081 aaactattat attatcacaa tttgatatgg tacttaatct atgagctata attatacttg
    10141 tctttccata agttaaatta tttattgctt cttgaatttt agcttctgtt gctatatcta
    10201 tgttacttgt agcctcatcc aaaataagta ttggagcatt ttttaatact gctctagcaa
    10261 tacttattaa ttgtttttga ccttcactta aatcttcacc cctatccgat aatacagtac
    10321 tatatccttc acgcatttta actataaatt tatgtgcatt tgctatttta ctagcatata
    10381 taacttcttc atcagtagcc tccagatttc cataccttat attttccata actgtatcat
    10441 tgaatatata accttcttgt ggaactactc caaaacaagt tctcaaatag tttctgttaa
    10501 tagagtatat atccttacca tctaaaaata tacttccttc atctatatga taaaaatttg
    10561 taattagatt tataatcgtt gtttttccac ttcctgtctc tcctatgatg gcatttgatg
    10621 tgccagcttt gatttttagg tttatattat ctaaaatttt cttttttgaa tcataagaaa
    10681 aacttacatc tttaagctca atttctccct ttacaaaatg agagtttaaa ttaccatctt
    10741 tattatcgtt aatctcactt ttaaacatgt tatgcacttc ttgctcttct tgttcatcta
    10801 taatttcaaa aactctttca catccagcta tggctgaaag taatgtatta aatagatttg
    10861 ctaattcatt taaaggtctt gtgaattgtc ttacataact taaaaaacta gctataaccc
    10921 ctatggttat aatatcttta gatgcaagaa cacctcctat aatacatata gttgcaaatc
    10981 ctatattact aatcacattc ataataggca ttaaaagaga agtgtaaatt tgcgatttaa
    11041 gggaaatatt tagaagtcta tcattgactt ctttaaaatc atttacagat tttctttcat
    11101 aattaaaaga ttttactaca tttatgtttg atatcatttc ttcaatatgt gcatttagtt
    11161 ttcccaactc aacttgttgt tgcttaaaaa aaactctagt tctctttgca ataaaattac
    11221 tcacaaacat tactatcggt attaatatta cactgcaaaa tgttaaaatt gggcttaata
    11281 caaacatcat aataaaagtc atcaatatgt ttaagattcc tgtaattaac tgtactaatg
    11341 aatttgatat tcctgtactg atattatcaa catcatttgt cactctactc ataatatctc
    11401 cagtagaact tttatcaaaa aaggttattg gtagattttt aaattttaaa aataaattac
    11461 ttctcatttc tttaactata ctttgagata atctagctgt acatatcccc ttagacagtt
    11521 taactgaaaa ctcaattata taaaatgaaa gtaaaataat tattcctttt aagaaaatgg
    11581 attttgaata agtatcgata tctattatgt caactacctt tccaatagta tatggtatta
    11641 cagttgatat aattgaatca attattacta gcaaaaaaat aaatctagta atcttcttat
    11701 cttttttaaa gtacataaaa attcttttaa acgttttttt aaaatcttta ggcttttctt
    11761 ttttctttaa tctactgacc ttttgatttc cagtacttaa gttcaatata cttcctcctt
    11821 aatttacatc ttcacttact aaattctgca acgcaaaaat ttttttataa attggagaac
    11881 taaccagtaa atcttcatgt gtagcaaatc caaccacctc accctcatcc aagactaaaa
    11941 tcttatcaca gtcaattata gaactaatct tttgtgaaat agttattaca gtacatttct
    12001 tactttcctt ttctcttaaa ttacttatat actcttttat attctttctt ataattgtct
    12061 ctgtattaat atccattgca ctaaaacaat catcaagaat taatatttgt ggttttttgg
    12121 ctatagctct agctatagta attctttgtc tctgtcctcc tgataaattt ttaccccttt
    12181 gaaataaaac ctcatcataa gaattttcga gtttagttat aaattgattt gcatttgcta
    12241 tctcacatga ttttataaca tcttctctac ttataccctc atttcccata actatatttt
    12301 ctagtatact ttttccaaac acactgctat tttgaaatac tacacctatt ttttccctta
    12361 aactttcttc agaaatactg tatatactac gtcctccaat cttaatatca cctttatcaa
    12421 ttatatataa tttttgaatt aaatttacta aagaagtctt tcctgaacca gtttgaccaa
    12481 tcacaccaat attttgacct tcttctattt taaaggatac gttttttaat attttttcat
    12541 ctgtattttt gttatacgaa aaatctacat tttcaaaaat tatatcacaa tttacaaact
    12601 ttaaatcttt ttctccaaca tctaaacttt cttctctctc aattacttct tcattctcat
    12661 caaccaaaac ctcacttatt ctctcataag atgcttttgc cctaacaaaa acattaaaaa
    12721 catgtgaaat cattataaga gatgttaata ttctagtcat atagtttaca taagcaacta
    12781 taatcccaat tttaatttct cctgaataaa ttaaatgcct tccaatcagt aatattatta
    12841 ttatggaaac attaatgatt attgtaattg tcggtctaaa aaatgacatc atagttgtag
    12901 cttttctact atttttaaaa agttcatcat ttaactcttc aaagctttgg ttctcataat
    12961 cgtatctaca aaatagttta actactttta ttccacttaa aaactgtctt aattttgaat
    13021 ttactttatc tgtactttct tgtacatttt taaataatct ataccctatc ttcatattaa
    13081 gcaatattat aataaatata attaaaactg caattagaat aattattgac atctttaaat
    13141 ttaattttat agctataatt aaacttccta tacacattat tggtgacttt ataaaaactc
    13201 tcatcattcc attaacgaat tgttgcattt gattgatatc attagtaatt cttgttatta
    13261 acgaagctct accaaattta tctatttgtt ttaatgagta tgtatttatc ttttcaaata
    13321 catcctgtct taaatcataa gaaaaatgct gagaaactct tgccgagatt acacttctta
    13381 ttattgcaaa tattgcacct aaaaaagtta tacctatcat tatagctcct gttgatagta
    13441 tgtagtctag gttcttattt gaaaccccta tatctattat ttttgccatt atagttggtt
    13501 gagctaaatc acaaaaagct tccaatgtta aaaaaaatac tgctgttaga aataatacta
    13561 tatatttttt tatgtatttt ttaaaatact tcattcaatc atcccttcat agaattttct
    13621 cattaccaaa agtatatcat aatggctaat ttaattgatt atatcaatta taaatatttg
    13681 atttattatt aatttttata ccatatattt tataaattat
//
LOCUS       DACTGW010000093        13359 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.093, whole genome shotgun sequence.
ACCESSION   DACTGW010000093 DACTGW010000000
VERSION     DACTGW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.093"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(11..769)
                     /locus_tag="I9573_002078"
     CDS             complement(11..769)
                     /locus_tag="I9573_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HAT4796698.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(771..1487)
                     /locus_tag="I9573_002079"
     CDS             complement(771..1487)
                     /locus_tag="I9573_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HAT4796699.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWAGKMIYCIICCSIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEMWYVFPYSIPSRLMVVVLHILPNGLPVPEGSELLNNAVILPGVLISV
                     ILFVLFALVTRAWFNNREVK"
     gene            complement(1489..2196)
                     /locus_tag="I9573_002080"
     CDS             complement(1489..2196)
                     /locus_tag="I9573_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013371337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HAT4796700.1"
                     /translation="MNNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRKLIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVIGYQGEVSKEQN
                     LEKLFMKVVAENRKRGE"
     gene            complement(2313..2849)
                     /locus_tag="I9573_002081"
     CDS             complement(2313..2849)
                     /locus_tag="I9573_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAT4796701.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(2951..4021)
                     /locus_tag="I9573_002082"
     CDS             complement(2951..4021)
                     /locus_tag="I9573_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HAT4796702.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDISVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSTSSYLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSNMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(4014..5072)
                     /locus_tag="I9573_002083"
     CDS             complement(4014..5072)
                     /locus_tag="I9573_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAT4796703.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNIKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSVTIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(5059..5391)
                     /locus_tag="I9573_002084"
     CDS             complement(5059..5391)
                     /locus_tag="I9573_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAT4796704.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            5590..6711
                     /locus_tag="I9573_002085"
     CDS             5590..6711
                     /locus_tag="I9573_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HAT4796705.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(6839..8737)
                     /locus_tag="I9573_002086"
     CDS             complement(6839..8737)
                     /locus_tag="I9573_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002846060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAT4796706.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            9052..9828
                     /locus_tag="I9573_002087"
     CDS             9052..9828
                     /locus_tag="I9573_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HAT4796707.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGVGDLKELYRNINYHKLPSSSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(9920..10672)
                     /gene="xth"
                     /locus_tag="I9573_002088"
     CDS             complement(9920..10672)
                     /gene="xth"
                     /locus_tag="I9573_002088"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAT4796708.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(10833..11783)
                     /gene="galT"
                     /locus_tag="I9573_002089"
     CDS             complement(10833..11783)
                     /gene="galT"
                     /locus_tag="I9573_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAT4796709.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            12168..>13359
                     /locus_tag="I9573_002090"
     CDS             12168..>13359
                     /locus_tag="I9573_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAT4796710.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQ"
BASE COUNT         4570 a         2198 c         1510 g         5081 t
ORIGIN      
        1 caatttttat ttattcttct gactttttac cttcccattt agaaaaccaa atacataaaa
       61 ttacaaatgc taaaattgtt acaaaaccac acattaaacc tgttgattga tatgctttta
      121 ttgattcaac ctttatatat tcaaccgttt tagaacttga aaaagacgac caaatagtta
      181 taaatctaac accccatgca caaggaaaat actgccaaat gccagaaccc aatccagtta
      241 ataataatgc tgaaagtaac atttcaacta tacctactcc aattgatgtt ccttttccga
      301 accttaaact taagaataaa tgtaatatgt attcaaataa attacaccca attaaaatta
      361 gaattgaaat catataaaag cttgatgtaa agtaagcctt agatatacca aatttaaaac
      421 ctaatacaga cactaatgtt gctacaaaac caagacatac taagattata tatttactta
      481 taagagtaag atattttgga ttagatgttg ttaacatatg tttataattc cctgcataat
      541 attcttgttc aacaaacatt gaacacacaa cacctgacaa taaaggaaaa gctattgcta
      601 taacttgtaa atatgctact atctttaata tattatcagc tcctgaaacc ttataatacc
      661 ataaaaacaa tcccatcccc attaaagcaa tgcaaatgtg cattagtatt ataaaatttc
      721 tctttaattt tattaaatca gaacaaaata ttcttattag ttgtctcata ttacttcacc
      781 tctctattat tgaaccaagc tcttgtaact aaagcaaata atacaaataa aataacagat
      841 attaatactc ctggtaaaat aacagcatta tttaaaagtt cactcccctc tggtacagga
      901 agcccatttg gtagaatatg caatacaaca accattaatc ttgaaggaat cgaatatgga
      961 aatacatacc acatttctgt agttgagaaa acagcaactc caacaatagt aaagaacata
     1021 tttgttagaa ctgtaacaaa tataccaaat tttgaagcta aaaacataca aagaggaatc
     1081 tgccacataa atagtaaaat aattaatata gttgcaatga tattttgacc tattgaaata
     1141 gtttctttat agattatccc aacaaaaact acacctattg cagaaataat cgatgctata
     1201 gaacaacaaa taatacagta aatcattttt ccagcccaaa cgtctttcat ttcaattggc
     1261 aatggtaaaa taccacgata tttacttttt tcatctttta atactattaa agaagatatt
     1321 aatgttaatg ctcctggaag aaatgtcgta taccaccagt tgaaagcagc attttgccct
     1381 ccacctagtc cacacaaaag tatacaaaac actggaacta tccatatcaa cttcattcca
     1441 aaagttcctt tcatcttttg aaattccact ctaataaatc tttccatatt actcccctct
     1501 ctttctattt tcagctacaa ctttcataaa aagtttttct aagttttgtt cttttgatac
     1561 ttctccttga taaccaatta ctccattaga aattattccg atttcatcta cgacttgttc
     1621 aacttcagac aagatatggc ttgataaaat aactgtaatt ccttgttttg gaaaagaacg
     1681 aatcaatttt ctaagttctt caataccaat agggtcaaga ccatttgtag gctcgtctaa
     1741 gattaaaagt tttgggtgat ttaataatgc aatagcaata ccaagacgtt gcttcatacc
     1801 tagagagaat tgacctgaac gctttttaaa agtatctctt aagtcgacag tctctaaaac
     1861 ttcttcaatt cgagagtcag gaagaccaag taaagtagta tggactttta aattttctcg
     1921 tgctgttaaa ttctcatata tagcaggaga ctcaataaga gaaccaatat ttgataaatc
     1981 ctttctagtc catctatgtc cctcaaacat tatttttcct gaagttggat ttaaaatccc
     2041 agttatcatt tttaataaag tagatttccc agctccattt ggtcctaata atccatatat
     2101 agaattagat ttaattgcta gtgaaatatt atcaactgcc atctgctcct taaatttttt
     2161 agaaactcct tttacctgca aaatataatt attcataatt ttcatccttt cttttgtcaa
     2221 acttatttac aagaatcatt ctaaatcata aatataagga ttttataaga attaaaaaac
     2281 aaaaaaaaga tgtgttaaaa acacatcttt ttttatctta attctattaa ttttccattg
     2341 tagtatatct ctacttcttc aatccaaatg ctattatctt cttcttggat gacattttta
     2401 ataatattat ttatagtata tattatattt tgttcattca aataatcatc tttccaagaa
     2461 actctacttt ttttatctgg ttctatcaag tttattttaa caagattatc tttttcctta
     2521 acttctactt ccatgtctaa accattaaaa tactcatttg ataatgtatc taagattaat
     2581 cgcagtttat ccttaagtga actttcttca tcaatctgta agtactttac tattttctca
     2641 tcctttgaaa ttatctctat aggtatttta gtagtttttt tagaattaat cattttacta
     2701 gttgtttctt cttttgcatc ttcatccttc tttacaactg ctgattcttt agaatcaact
     2761 agctcaactg cactgatgct ataatccata catgctgtac tcaataaagt agtagcaatt
     2821 attaaaaaac ttactgctat tttattcata agtatccctc tcctaattct ctttcgatac
     2881 ttattatacc atagtataga ttatattttt atacttttaa atcaatttat aaattattat
     2941 agtaaattat ttacaattct aagctatttg aatttatttt tatagtatat tctttctcta
     3001 aattcgacag atttttattc aaaagccctt ttatttccct cgtatttcta tacttgttgc
     3061 tatgttcttc actatgttca taatcatatg aatcattgtc atattcttca tcattattaa
     3121 attcttcaat tgcttctttt gactttatat cacaattaaa tttatactta tttactggta
     3181 tatcaagtct taccttagaa tttggaactt ctatgtccag gaacttagca attggcatat
     3241 tagagtacaa gtaaaattca ctttcataat ttgaatttct acctattttc tcttttatat
     3301 ttacattttc aggtatatac tcttctatat tattgataaa tacatcttca ttctctgaag
     3361 atatatccaa tgactctgat tctatgttta catttttaac gcctaaaatt gattttgttg
     3421 acaagtgaag attgctatta gatattaagt ttaggttttc taatctattt actttgttct
     3481 ctgttatagc tctactaaaa cctccataag aagttttata tgttatagta tttgaagcta
     3541 cattatcata tacaaataag tatgaacttg tacttgatgc atttatgtct acattatttg
     3601 gtacatatat tataatttct ctataatcat gtgaaaacat actatttata gatgtattta
     3661 ataattggtc gaaattttta atctttttaa ttttattttc atattttctt tcacctttta
     3721 caactaaagt cttatctttt aaagttacat tgtatttgta aaactcatta tttcctcttg
     3781 ttgttacacg cacatcttca cttgaacttt tctttattgt aacgtttata ttgtcaacag
     3841 atatatctaa cttgtcaata tttacttttt cttgataaag tttattttct tttttaaatt
     3901 cagcttcctt ttctaaacca aagtttatta ggcttggcat aactaaaatc cctgaccatg
     3961 ttgtccctat tatacctatt accaataaaa ctgcagcaaa tatagcaagt tttttattca
     4021 tgtttatctt ctcctttctt attttctata tcttcaaggt ccttatatat cgcctcaaaa
     4081 tttatactat catcattata ttcatcagtt tcttttatgc tttcttcttc tctattgtag
     4141 taatcattat tatatgaatc ttcattaatg tctttattca aatcttcatc atttaaagta
     4201 gctttttttc ttttttccat cttttcattt gcctttatat aaatattttt tctctttatc
     4261 caatgtatat aattatttag taattgttta cctagtttta tagcaaataa taaaatttgc
     4321 catgcaagta tctcaagccc tacaaatgct atggttgcga aaatacaaaa catcataaca
     4381 tctggaacca ttgctataaa tttaatcaca aatggaatta atataacaaa agcaattaat
     4441 gaacatacta atgcaatagt aacagataca atagcacata caaacgcaaa tgctggaaca
     4501 attagaaata aagataacac tactaataat ccctttataa cttgtctaga taatccccct
     4561 acaccttctt ctatacttgg tgtaagttta tcatttatat tttcttttga tttattgtac
     4621 ttttccttag cttcatcttt caagatagag taatcctttt ttatatttga tttaaaccta
     4681 ctaaatttac catttaatat atctgtattc attatttttc catctatatt ttcttttgat
     4741 ttactatact tttcttttgc ttcatctttt aagatagagt aacctttttt catatctgat
     4801 ttaaatttac taaacttatc attcaaaaca tctgtattta ttattttttc ctcttcaata
     4861 tttttttctt tagtctgcaa gataagttct ttggctatag attttggtga accaagtgat
     4921 gctattatct ccatatcact ctttccctct ataagaccat ctacgaaata ctcttcataa
     4981 tctctcaata tatcattaac ttcgctctca gaaaaatctt tttttagata atcctttaaa
     5041 atttcaagaa actcagtttt attcaagttc attacctccc tcatcttcac ttattaaaat
     5101 ctcaacagcc tttacaaatt ctctccactc ttccatcaga ttggataaat tctcttttcc
     5161 tagtgaagtt attctatagt attttctagc aggaccttca ttagattcta gtatatatgt
     5221 ttcgaaatac aaatcttttg taagtcttct tagaatagga tatattgttc cttcatttac
     5281 atctattact ttagagatgt tatgtactat ctcatatcca tacatatctt tcttagatat
     5341 gagagcaagt acacaaattt ctaaaacacc ctttctaaat tgtgtgttca tatactccat
     5401 cctttcttta aatacacagt attgtgtttt acaaggtact atgttaaaac ttcttatatc
     5461 ctcataataa cacagtacta tgttctgcac aatagttatt tttaaatatt cttgttgatt
     5521 aaatccttct ataaatgtat aatactctaa tcagatatat tttatgaaag tggtgataaa
     5581 ttggctaaaa tgaaagatga taaaacaata ttaatcttaa ctgctcaatt tggtgctgga
     5641 catataagtg ctgcaaaagc agtcaaagaa tgtattattg aaaaatatag taactataat
     5701 gttgttatac aaaactttat aaatgcaagt attcccatga tgaataaacc tatggtaaaa
     5761 ctttatgaaa acaatacaaa atatactcct ggattatata attactatta ttactttaag
     5821 aaatcgtttg attcaagaca tgatttatct cataaattat atacacctaa actttctgag
     5881 tatattgctg atataaatcc agatttgatt atctctacat ttccactagc ggctgcttgt
     5941 gtaaataact ttaagataaa aaatcctgat ataaatatac ctacacttac agttataaca
     6001 gatgttgtag atagtatgga atgggttttt gaaaatacag atttgtattt tgttccttct
     6061 cctgaaataa aaaatagatt ttttcagaaa ggaataaatc cagactccat aaaggttact
     6121 ggagttcctg ttgataagag atttcaaatt gaaagtaaag aactatgttg tgataaatat
     6181 agactattac ttctaggtgg aggaagaggc ctatttgata tagatgagga ttttatgcat
     6241 tggatagatg aatttattga agaacatagt aattctatag aaattacaat agttactggt
     6301 aaaaacaaaa aattatatga caatctaact cataaaaaac ctctaaaaaa tattaaagta
     6361 cttggatttg taaacgacat gtataatcta ataagagaat gtgacctaat gctaactaag
     6421 cctggaggag caactatatt tgaagctatt caatcacaaa ctccagtact cgtgaaaatg
     6481 ccaaaagttg gacaagaaat tgaaaacgct aaatttataa tagataaggg tcttggcatg
     6541 atttatagtg atgacctaga ccttaaaaac atattttata aattagtatc aaatgaattt
     6601 gattctataa taaactttat gaaaaaaaat ttagaagaat ttaaaacagt tattcatcca
     6661 gagaaaattg ctgattatat atcagaatta atagataatc attacagtta aattgtacta
     6721 ttaaaaaatt tcaaacataa aattaaaggc aatcaagtat gcttttacac ttgagaattt
     6781 actcatagtg tttaataata cttgattgcc tttatcatat caaaatcaaa ttgttatatt
     6841 agtatatatt tttcttgtac tgcatataca gttgttgtat ttcttccata cttctcttta
     6901 attctatttg taaatctgaa gcagtctggt aatccttatt ttctatagct tctctcactc
     6961 tagtgaatag tgattctcta gttaaagtat ccttcataat attgaacttt aaatccgata
     7021 ttgagttcca aactacctca tcaagcgcat ccatattttc ttttgtgtgt atcttgacaa
     7081 tacttcttat tttttgaatc ccagcctcta ttattctagt ttttaatttt ttttgccact
     7141 tatctatagc tccaatctta aatgattcta ttatactatc agtaaataca tcaccttgtt
     7201 ttaaagattt taattttgaa gcatatattt ctaagttttt catattttca taaactgtag
     7261 ccggtggtat tccaaatctt gcatttcttt cttccaaact ataatgttca aatacatcat
     7321 tttcatctct atatgctcta tctttttcta aatagaaact ttcttcacct acatttttag
     7381 ataactcttt ttcaagttct tttgtactaa gaccactctt agctgctgct tttataccat
     7441 ccaacattgt ttgataacat cctgctatta ctaaatacgt gttagataga gggcttggtg
     7501 accttaactc aaatcttaca gtctttggat ttttcatatc tcttattaat ccaacaagca
     7561 ctgacctatt tcttgatgga atctcataac tatgccctaa agatgtaact atgcatacag
     7621 gagcttcaaa tccaggaact aatctattaa atccatcatt agaagctgtt acaattgggt
     7681 ttaatacttc ataattgtaa agaagtccca taagagcccc atatcctagt tcacttagat
     7741 aatcttcttt taaatctttt ggagcaaata gattttttat acttccatcc tttaacttcg
     7801 cacttactcc tacatgtgta tgtccaccac ttcctgctac tccatgaatt ggctttgctt
     7861 taaatgtaac ttctagtcca tgtgatgtaa atacatcctc tataacatct cttactaata
     7921 attcattgtc tgctgcttgt aatggtgtag aaaacttcca agaaacctct aattgctcca
     7981 tagcatggtt tgttcttcca tctatactaa tagaactttg tattcctccg acctctttgt
     8041 gagccatttc agggtttaca cctagctttt gaagtataat taaacttttc tctaaacaag
     8101 ttcttataat accatgagtt cttttccagt attgctcttt taggctttgt gatacatata
     8161 atttctctaa gtcagcttta tcctcaggag tattaaccca gaactcaagt tctgtagctg
     8221 ctgttaacat tatttcctct atatcatcta ctgaatctat tcctatgtta tttattacat
     8281 gtggatattc tctaaatatt tcatacattg actttttaaa gtacttatct gctttttgaa
     8341 gtactcctct agaacatact tttttattat catgtattaa aaatgcaggt atctttaatg
     8401 ttccaacagg tagacctact tcttcgtcta tatattccat attgtaatct acataccaat
     8461 gacaactttt atctggcatt aaatctactt tcgcattatt taatgtagct atattataaa
     8521 gctcaacact tgaaccatca gtctgtatag ctgattccaa aaatttatct atatcctcca
     8581 aaaataattc tacaggaatc ttttcatctg ttgcatttcc tcctaaatca acccccatta
     8641 aggaaacaaa tcttatattt ttattgcttt ttaggatttc tcttaattct tcattagtgt
     8701 gtttatcttt ctctattacg tataatagac tatccatttt tccactctcc cataattttt
     8761 atgtatattt tattttgtta aatacaagat aaatttcatt ttccttacat tcatgaattt
     8821 ttcttgcaaa ttattcgtgt tttattcgtg tttttattta tattattata tatcaagtat
     8881 aaaaagttcg caatattttt tattggaaaa aatattatac atacaatagt ttaacatttt
     8941 tacaaatatg gggtatacta aaataaaagt atttttattt gaaaatttaa ttttatttat
     9001 aatataatct atagtaaaag aaataataaa aaaatttagg agataaactt tatggaatac
     9061 ataattttcg atttggaatt taaccaagga tttgataaaa aactcaataa aactgtatcc
     9121 aatgaaaagt gtccttttga aattatacaa ataggagcaa ttaaattgga ttctaaattc
     9181 aatataatag atacgtttaa tagctatgta aaacctacaa tttataaaga catacatccc
     9241 tttattagtc gtatgactaa tattaaaaat tctgatttta atgattcgcc tactttccct
     9301 gaggtctaca acaattttat aaaatttata tcatcacaag accctatatt atgtgtttgg
     9361 ggagttggag atttaaagga attatacagg aatatcaact atcacaagct accatcaagt
     9421 tccttaccta aatcttatat taatatacaa caacatgcat ctaaatattt caataatcca
     9481 gcaggaaaaa gcattggcct acaaaatgct atttctatat tggaacttga tgaaaaaatg
     9541 tcttatcaca atgctttaaa tgatgcatat tatactgcta aagtttttat taagatatac
     9601 aatccaagta tagtcccaga tatatatctt tatacgagta taaaacctaa aactataaga
     9661 tattctaata aaaaaagagt tgattatgat aagttatttg atgaatttag gaaaatttta
     9721 aatcgtgaat taactaaaga cgagaaaaaa atcattaact tagcttataa tatggggaaa
     9781 acaaatcaat ttacattaga aaatgttaag caaagaaaaa acaaatagag tttatttaga
     9841 taaaaaatag gcatgtagat taaaaaacta gttaacttta aatttatatc tacagcccaa
     9901 tttttatcgt aattaatttt tattttatct ctaattcaac tggacaatgg tctgaaccta
     9961 atatttcagt gtgtatatcc gcacttacta atctatcttc taattttttg gatgcacaaa
    10021 aataatctat tctccaccct gcattatttt ttcttgcatt aaatctatat gaccaccaac
    10081 tataaacgcc ttctttatca ggattaaaat atctatatgt gtctataaac ccactgtcta
    10141 ataatttaga aaacttctct ctttcttctt tagtaaaacc tgcatttttc atattatttt
    10201 taggattttt taaatctatt tctttatgtg caacgttcat atctccacac acaattacag
    10261 gcttatgatt atctagtttg gttaaataat ctataaagtc gtcttcccat ctagttctat
    10321 attctagtct ctttaattca gattgagaat ttggcgtata tactgtaaca aagtaaaaat
    10381 cttcaaactc tagagttatc actctaccct ctttgtcatg ttcttctata tttattccat
    10441 acattacttt taaaggctct ttttttgtaa atatagcagt acctgaataa ccctttctct
    10501 cagcataatt ccaatattga aaatatcctg gtaaatctaa ctctatttgc ccttcttgca
    10561 atttagtttc ttgtaaacaa aatatatctg catctacttc tttgaaaaag tctaaaaatc
    10621 ctttcccaac acaagctctt attccattaa cattccatga tataaatttc atacttatac
    10681 tcctcatcta aacattgaca cactccccat agctaaagat gggggctttt caacaatttt
    10741 attgtaaatt cttaatttac ttttatttta acaatactat actataattt catctataat
    10801 tactatttac ttaataccta aaaatatatg ttttaatcaa tttttaactt ttttgttaaa
    10861 tcttcagggc tcattgaaga aacatacata tttgttccaa gttcaaatcc tccaaaatta
    10921 tattttcttg ggattatgtg catatgaaca ttaaaatacg attttgtttc aatctttgtt
    10981 ggatgagtgt gaatacataa gttaaatggc atatatccat tgatattgta taacttctta
    11041 aacaactttt caaatattgt agaaagctcc tgtatattat tatcatttaa ctcaccaaac
    11101 tctattttat ctttaaatat tattcttact tctccactat acttggttgc atatggtata
    11161 agtaccaaaa aagcttctcc attataaaca actcttctat ctaaacttat ttcattttct
    11221 attaaatcat catacaaaga gcttttattg ctgtcataat actcctttga aactaacagt
    11281 tcattcgtaa tttcaggtgg aataaaagac atagacaata tttgagcatg tggatgcatc
    11341 aaagatgctc ctgcctttct caagaagttt ttgaagatac taacatactc tactttatca
    11401 tctttcgaca aatctctaaa tctatttcta tacataaaaa aaacatcttc aaattctttt
    11461 tgactcatgt tataaaaact acgattatgt ttatcgctct ctatcattac ttcatgaaca
    11521 ccatatacat ctcttgccat atcatcaatt ataggaaatt tattgtagac agattttaca
    11581 agccatcctt cttcactttc tatttcaaat aatgcatcag ttgcatgagc ttcatttcct
    11641 ttgcaaaatg ggcattcttc atcatattca ttagtttctt catcttcatt tgggatatct
    11701 gctgtatcta aaggtctttt taacctatct gtagcaaata taacaacatc attagtaatt
    11761 ggatctattc ttaattcttt cataattaac acctcttagt taatagcctt taatataatt
    11821 atatgatatt tatcgtatct gttaaataaa tttataatat tttttataat tttgcatatt
    11881 tattattata tattttgtct atagctgaaa gattataact aaaaaatcac tatcagtgta
    11941 tcagaatatt atcttactat aaattaattt atgaatacat aatttagtgg cataaacatg
    12001 ggtattttac tataatttgt taaaagttta ttttaaagct atattaattt tttttgatga
    12061 tttttaccca taagtatgtt attattatct tgtaaagatg tcttgtattc aaacatacaa
    12121 tttacataat acaatatacg tatttaaatt aaataggggg tattaaaatg aattattcaa
    12181 acagagttag tgcaatgcaa gcctctccaa tcagaaaact tgttccattt gcacaagcag
    12241 caaaagataa aggaatcaaa gtatatcact taaacatagg acaaccagac ataaaaactc
    12301 ctaaaggatt ttttgatgct gttaaaaact ttgatagcga agtcttagaa tatgctacat
    12361 cagaaggtat tccagaatta ttagaagcac ttcaaaatta ctataaaact tacgatatga
    12421 attttgaaaa agatgaatta ttagtaacta atggtggtag tgaagcttta cttttcacta
    12481 tgatggcagt ttgtgaccca ggagataatc tattagttcc agaaccattc tatacaaact
    12541 acaatggatt tggtcaatct gtaaatgtag aagttaatgc tgttacaaca aaggcagaaa
    12601 acggtttcca ccttccatct aaagaagaaa tattgtctaa agtagatgat aaaactaagg
    12661 ctataattct ttctaaccca ggaaatccaa ctggtgcaat ttatacaaaa gaagaactta
    12721 atatcctagc tgaaatagct aaagaaaaag atttatggat aatagcagac gaagtttata
    12781 gagaatttgt ttatgatggg cttgagtata caagctgtgg taacttagag ggtgtacagg
    12841 atagagttat aataatagac agtgtttcta agagatacag tgcttgtggt gctagaattg
    12901 gttctatcgc ttgcaaaaat aaaggtctta tcgctcaaat acttaaatta tgtcaaggaa
    12961 gactttgtgt tcctacatta gagcaaatag gagctgttga gttatacaaa actcctgttt
    13021 cttacttcaa agaagttaat gaagagtaca aaaaaagaag agatgtatta tacaatgaat
    13081 taatgaaggt tgaaggtgtt atatgtaaaa aaccaactgg tgctttctat atagttgcta
    13141 aattaccagt tgaaaatgca gaagatttca caatttggat gttaaaagaa tttaacaaag
    13201 ataatgaaac agttatggtt tgccctgctg aaggattcta tgctactcca ggtttaggac
    13261 gtgatgaaat aagattagct tacatcttaa atgaaaaaga ccttcataga gctgctacat
    13321 tattaaaaga aggtttagag caatatgtag ctttacaaa
//
LOCUS       DACTGW010000094        13280 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.094, whole genome shotgun sequence.
ACCESSION   DACTGW010000094 DACTGW010000000
VERSION     DACTGW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13280
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.094"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            105..1535
                     /locus_tag="I9573_002091"
     CDS             105..1535
                     /locus_tag="I9573_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4796711.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1638..2741
                     /locus_tag="I9573_002092"
     CDS             1638..2741
                     /locus_tag="I9573_002092"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAT4796712.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMTYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            2756..2992
                     /locus_tag="I9573_002093"
     CDS             2756..2992
                     /locus_tag="I9573_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAT4796713.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEKACQEA"
     gene            3009..4058
                     /gene="vorB"
                     /locus_tag="I9573_002094"
     CDS             3009..4058
                     /gene="vorB"
                     /locus_tag="I9573_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HAT4796714.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4060..4800
                     /locus_tag="I9573_002095"
     CDS             4060..4800
                     /locus_tag="I9573_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HAT4796715.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            4803..5342
                     /locus_tag="I9573_002096"
     CDS             4803..5342
                     /locus_tag="I9573_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAT4796716.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLLEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5466..7565
                     /locus_tag="I9573_002097"
     CDS             5466..7565
                     /locus_tag="I9573_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HAT4796717.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKMIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWALDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNTKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(7832..8347)
                     /locus_tag="I9573_002098"
     CDS             complement(7832..8347)
                     /locus_tag="I9573_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HAT4796718.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8439..9650)
                     /locus_tag="I9573_002099"
     CDS             complement(8439..9650)
                     /locus_tag="I9573_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HAT4796719.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDFPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            10326..11768
                     /locus_tag="I9573_002100"
     CDS             10326..11768
                     /locus_tag="I9573_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HAT4796720.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            11810..12163
                     /locus_tag="I9573_002101"
     CDS             11810..12163
                     /locus_tag="I9573_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796721.1"
                     /translation="MKKSNIFSIIFPTIIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            12354..13175
                     /locus_tag="I9573_002102"
     CDS             12354..13175
                     /locus_tag="I9573_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HAT4796722.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIKKVKSNNIA"
BASE COUNT         5039 a         1565 c         2405 g         4271 t
ORIGIN      
        1 aaaaatatac tcctgaatca agaatgagat atttaagatt caaaatatat attattatac
       61 gtgaataatg aaaagtaaga aaattttata agggaggggt agatgtgagt agtcaagaaa
      121 gcaaaccaaa aggaaatctt atatcaaatt ttagagatga tatgagagac cctaagaaaa
      181 gagcaatata cacatattca agttctataa acttagcgta caattttata ggttactttg
      241 tatcaactgt tacaaatatt tttttaactg atgtggcaaa acttggtgta attatggcag
      301 gtggtatgca aattatacaa tctttcataa aaattgctgt agctcctata acaggtacag
      361 tatttgacaa acaaccattc aaaaaaggaa agtattatcc atggcttaga tatgcaccag
      421 cagcgttagc attaacatat ggaatatttt tcatagttcc attaatgaaa ataagtccag
      481 cattactttt acctttgatg acagctttat tattaatagc atcttgtgct cagcaagtag
      541 ttttcactat aatttatgct atttaccctg tagttgctaa gacacctaga gaccgagtta
      601 taggctcaac tactactaat atatttaaag aagttggtaa attcttagta ggtttttcat
      661 atccgttatt attagtttta tttgcagatg tattaggtgg agaaggaatg ggttatttag
      721 gtacctactt attctttgca gcaggatgcc ttttagtatt ttggttttct tcaacagaga
      781 taataaaatc tggagcagaa gaagaagttc ttcaaaaaga agagaagcca aagattaaag
      841 caagtgaaat ggcaaaagct ctattcacta atgtaccact tatgatggca ttttgtttag
      901 aatttcttat atgtataaga tctataggat taggaccttt ggctccatat tattttaaat
      961 atgtaataga agatgaacgt ggattagcag tatttttatc aatcatgcct ttggtaagtg
     1021 tagcattcat gtttttcgca ccagtgttta taaaaatctg tagagagcaa aaactagctt
     1081 caataatttc tttttctata tgtgcactat gccacttatt ggttgcatgg actccttggg
     1141 ggaaaactac tttaggagta actatgttgc tggcaattgg tggaggtttt tcaaatgtag
     1201 tatcaataat aaatcttaat ttctttgccg gttcttgtga ttatgggcat tggaaatcag
     1261 gaaaagactt acctggctta agtatgtcac tatatccagt tgcaatacaa gtaggagtat
     1321 tacttgcaac tactataaga acagttttaa tgaactcaat gggatatcaa gctgatatgg
     1381 tagtcacaga agcagttaaa tcaggtttta taaatatgat ttcttattct atggcaatac
     1441 ctttaataat agctgttgtt atagcaatac tatatccagt aagtgacaag aaattaaacc
     1501 aaataagaga agagttaaat caaagaaatg cttagttaaa agaaagttaa gtagatggtt
     1561 aatttaagct attaaagtaa gtagacgcaa tagcgtttat ataaatcaaa atacatatac
     1621 agagggggct tttaaaaatg atagaaaaat tattgagaaa taccgtaaaa gaattacatc
     1681 aatatgttcc aggtgaacca atagaaaaag ttaaggaaaa atatggtgta aaagaaataa
     1741 taaaattagc ttcaaatgaa aatcctctag gaccatcacc aaaagcaata gaagctatga
     1801 ttgaaatgct aaaacaaggt caactttatc cagagccaga agctaacgaa cttagaagaa
     1861 aattagctga aaagctagat ttaaaaccag aaaattttat tgttgctaat ggtgcggata
     1921 acgttatcac tctaataggt gaggctttta taaatagagg agatgaagtt atatattgta
     1981 acccaacatt tccatcatat agaactgcaa ctataaaaaa tgaagggata ccagtagaag
     2041 tgcctttaac agaagattat aagtatgatt tacaaggtat attagataag attacagata
     2101 aaacaaaatt aatatgtgta tgtaacccta ataatccaac aggaacaatt gtggatgata
     2161 aagagttaga agaattttta aagaaagtcc ctgaaaatat tataactata ttggatgaag
     2221 catatataga atttttaaca gttcctaatt atgtggatgg acttaagtat gtaagggaaa
     2281 attataacgt tatagtaaca agaactttct ctaaaatata tggtcttgca gggcttagag
     2341 taggatatgg tattgctaaa gatgagataa taagaacttt atttacagta aaagagcctt
     2401 tctctgcaaa tcgtgttgct ataagtggag caacagcagc acttgatgat gaagagttta
     2461 taaaagaaac atatgaatta aatagagttg gaatgactta ctttaaagaa gagtttacaa
     2521 aaatggggtt tgatgttgta gattcacaat caaatttcct ttatgtagat atgaaaacag
     2581 atatacctaa gttatttgaa gacttgaaaa aaagagggtt tgtaataaga ccaagtgcta
     2641 cacatgcacg tgtaagtatt ggaactatgg aagaaaataa gaaatttgta gcagttttaa
     2701 aggaaatatt aaatattaaa ggagagccaa gtattggata atagatagaa atattatgaa
     2761 acggggtgta gatatgaact taaaaataaa cgcagagaga tgtaaaagtt gtgaatactg
     2821 cgttatatct tgtaaaaaag gagcattaaa aatatcaagt aagataaata aagaaggtta
     2881 tgctcatgta gaagttgatg agagtaaatg catactttgt ggaatttgct atcaggtgtg
     2941 tccagataat gtttttgaaa taattaaaga aaaagcatgt caagaggctt agaaggagtt
     3001 gggataatat gaaaaagatg tggaagggga accacgccat tgcagaagca gccctaagag
     3061 gaggttgtga attttatgca ggatatccaa ttacaccaca aacagaggta atggaatttt
     3121 tatcacatag aatgtctgaa cttggaagaa cttttataca gtccgaaaat gagatggctg
     3181 ctatatatat ggtatatgga gcatatgcaa gtggaatgag gtctatgacg agttcttcag
     3241 gaccaggaat atctttaaaa caagaaggga tttcttactt atgtgcaaat cattaccctt
     3301 gtgtaatcgt aaatgtacag agatgggggc ctggtcttgg ctcattggac tcagcacaga
     3361 cagactatct aagagatact agagggggag gaaatggaga ttatcatttg atagtttatg
     3421 caccaaacag tattcaagag actgtagatt tgatgtataa ctcatttgat gtagcggaaa
     3481 aatacagagt gcctgtcgaa attttgacag aagcagcttt aggtcaaatg atggaaccag
     3541 tagaatttcc agaatttaag aagagagaag gagatttagg ttggacttat gatggaagta
     3601 atagagacca tgcaaaggtt gcagataacc aaaagccaac tttttgtatg gagaaacgta
     3661 tgagaataac tgaaaatgaa caacaatggg aagattatca agtagaagat gcagaatatg
     3721 tgtttgtagc gttcggtata ccatcaagaa caactatgaa tgctgttaga agacttagag
     3781 aacaaggtga aaaagttgga ataataagac caataacagt atggcctttc ccatataaag
     3841 catttgaaaa ggtaagtaaa gatgtaaaag gatttatctc agtagaatca acagacacag
     3901 gtcaattagt agaagatgtt gctttagcct caaagaaagt atgtaaagaa aatgtacctg
     3961 tgtatggttt atttagtgga aatcatatac caaaaacatt gcaagtaatg gacacttata
     4021 gcaaaataaa atctggcgaa atcaaggagg tgttctaata tggctacaga aaaattaaca
     4081 cctagcgttg tttcaatacc aaacaagttt tgcccaggtt gtggtcatgg aatagtaaat
     4141 cgtataatag cagaggtaat agaagaacat ggatatgaaa aaaatcatgt attaacatta
     4201 ggagttggtt gtgtctgcaa tatgaatttt agctggaatg gtgataagat gcaaactgca
     4261 catggtagag cttcatctac agctatagga gttaaagtag cacttccaga tacattagta
     4321 atgacttacc aaggtgatgg agatgcttat gttataggtc tttctgaaac tttaaataca
     4381 gcatatagaa atcataatgt gactgtattt gtaataaaca ataataattt tgcaatgact
     4441 ggtggacaaa tgtcatggac cactatgccg ggtcaagtca caacaacaag tgtaaatgga
     4501 agagatataa agactactgg tagtccactg aaagttcctg agatggtggc aaacttcttt
     4561 gatgtagcat atgtagcaag aggttcagtt catagcccaa aggaaataat taatctaaaa
     4621 aaatatataa aaaatgcaat agaagctcaa ttaaatggag agggatattc cttagttgaa
     4681 atattagcac cttgcccaac taactggggt gtatcactag agaaatctat caaatggatg
     4741 gaagaagaaa tagtgccata ttatgctctt ggcgaattta agcaaaggga tggtgaatag
     4801 ttatggaaaa agagctagta tttgcagggt ttggtggtca aggagtttta actcttggtc
     4861 tgattgtagc tgagtcagca cttgaattgg ggaaacaagt aacttggatg cctgcatatg
     4921 gtccaactat gagaggtgga aaagcctata gtgtagtaaa gttttctgat gagagtatag
     4981 gtgggcctga tatggaagaa atagatgttt tagtagcaat gaataaacca tctttagatt
     5041 atataaattt agttaaagaa ggtggaacag tagttattaa tacatctgca atagatgaga
     5101 atgtaacatt aagagaagat ataagtgtag ttaaaataaa ttgtcaggaa ttagctcaaa
     5161 aagtaaacaa tccaaaagca gcaaatatag tagtacttgg agcattaatt gctaaaactg
     5221 ggttacttga aaaagaatta gctttaaaaa ctatgtgtga tttctttgag gaaaaaggaa
     5281 aaggaaagtt taatgttcaa aatgaagcag cattcatgga aggctataat aacgccattt
     5341 gataaattac ccttatctta agtgatttat ctcaagattt aataatcttt agtgccaaga
     5401 gaaacttcat cttgagataa aattcactta aaatcacaac tataaacata taggagcgtg
     5461 aatttatgag tttggaaaaa cttattagac caaaaagtat tgctatagta ggagtgactg
     5521 ataaacttgg ttttggaaga agtgcagcac ttagtatagt aaaaagtaag gaaacagata
     5581 gagtatatta tgttaatccc aaaagagaag aattgtttgg gagaaaatgt tataagatga
     5641 tacaagaagt tccagaagta gtagactgtg tagtagtatg tacgcctaga aatgtagttc
     5701 catctgtatt aaaagattct ggagagttag gaacaaaggc agcagtggta tatgctagtg
     5761 gatttgcaga ggaaggtaca gaggaaggta cagatttaga aaatcaactt atagaaatat
     5821 caaatacata cgatatgaaa atattgggtc caaactgcat ggggttgcta aattgtatag
     5881 ataaggttaa tctatgggca ggaggttcta aatgggcttt agatactaaa aaacctggta
     5941 taggaattgt aggtcaaagt ggatttataa ctgctgaaat tgtttcaagt gattatttta
     6001 atatatctta tggtttttca acaggaaatg gaaatatagt tacactagaa gatgcagtag
     6061 atttccttgt agatgatgat tatgcatcag taatagcaat ttaccttgaa ggtttaaaaa
     6121 atcctcaaaa atttatagat gctttaaaaa gggcagcaca aaaaagaaag ccagtaataa
     6181 tattgaaatc aggaaggtca gaaaaaggtg ctatttctgc tgcctctcat acaggaaatt
     6241 tagctggttc gagtaaagct tttgaaagta tttttgaaaa atatggagta atttctgtgg
     6301 aaaatttaga acagtttatg tgtttagcac aagcttttag tgttttagat ggtaatttac
     6361 ctgcaaattc taattttgca gcaataaact tctcaggagg agaaaatact atttgtgcag
     6421 atttagcaga agaaaatggt gtagaacttg cagaaatatc ttctgaaact aaagaagaaa
     6481 tgaaaaaata tcttccagga tttgcaacac ctaaaaatcc actagatgct acaacagcat
     6541 tattccatga aaaggatatg atagtaggtt tattacatac ctttgaaaag gatgatagtg
     6601 ttggatttac tatggtaggt gcaaatataa gagatgaaga aaatgaaatg catgaaaccc
     6661 tttgtaaagc tgtaagtgaa gctagagaac aaggattgaa aaaacctgtg tttgcagttc
     6721 ctacacttga aggaaataga tatttagatt ataggactag attagaagat aatcaagtgc
     6781 ctataatgtc atcagttgga actacattta cttgttttaa taaaatggct aagtttatag
     6841 attatgatta ttcaaaaaga actttagaat ttaaagctgt taagaaaaga gagtcaaata
     6901 atgtagtagc attatcagaa ttagatagta agattgagat gaaaaaatat ggagtaccag
     6961 ttccaggaca aggtaataca aaaagtattg atgaattaga tgaaatctta aaatatatta
     7021 agtatcctgt tgttttaaaa ataaattctt ctgaaatatt gcacaaatct gatgttggag
     7081 gagttaaaat aggaattaag aatagagatg aggcagtaga tgcatataat gagattttaa
     7141 ctaatgtaaa aaaagcaaaa ccagatgcta atattgatgg tatattagtt caagaaatgg
     7201 tagaaagtgg tatagaaatt attattggta taactaatga tgaccaattt ggaccaatgt
     7261 tattagtcgg tttaggagga gtatttgtag aggtatttaa agatactaca ttatatccat
     7321 tacctataaa tcatgatgaa gctattatga tgcttaaaaa actaaaatca tttaagcttt
     7381 taaatggcta tagaggaagt gagccttgtg atattgatgc tcttgcagat atgatggtca
     7441 aattaggaaa atatgcttat gaaaataagg atgaagtcaa ggaaatagac ttaaatccag
     7501 tgtttgtata tcctaaagga aaaggcgtat gtgcagtaga tgccttaata gttaaatata
     7561 aatagtttat aagttttggt attcaaccat aattaaagca ccgactgaaa atcacatttt
     7621 gtaagttaga tagtgtgatt ttcagtcacc ccaaatttaa tttaaaaata ttttaaaatg
     7681 aaagtatagt tttgagttta attgtgaaat agcactgtat aaacaataaa atactacgta
     7741 taaaacgaat aataaaaggt gttttaatga taagaatcaa tcttgatttt cattaaaaca
     7801 ccttctttat tatattaaaa atttttaaac gttatattgt cacaggtggg tttgttggag
     7861 ctgcatatgt acgattagca aattcttggt caacaaagaa tagaccaccc tctccagcaa
     7921 gtttaacacg ttttatattg tcttcacaat ttgtttcttc ttctgcttgt tcagatataa
     7981 accaaagtaa gaattgagaa gttctaaagt ctgattcaga gtttgctaca gctgctaagt
     8041 tgtttattaa agctgtaacc tttttttcat gagctaacgt acgctcaaga atatccattg
     8101 cagaagtaaa gtctgaatct ggcattggga tagcatcaag ctgaaccttt cctcctacat
     8161 tatgtaagta tttgtaaaaa aacatagcgt ggtctgtctc ttctttatat tgaacataaa
     8221 accaattaga aaatccttta agtccttctg cttcaaggta agaagacata gaaagataaa
     8281 gatacgcaga atataattca tggttaatct gctcattaag taatttttcc attttagctg
     8341 aaatcatttt tatacctcca aattttaaat gctcttataa aatataataa tattaaatgg
     8401 aaaaatattc aataaaagtg tttaaatatt ttacatattt aataaaaatt tatactacca
     8461 ttaaaaaaat tataacttac aatggctata gagccaatta atggtgcatt tgctccaaaa
     8521 tttgaatgcc ttacacttat ttttttatat ttagaaaaac ttgctgaagc agtcaatttt
     8581 tttaataatg tattttctat aaatgtacca ggtatattaa agtcatagcc tattattata
     8641 gaactacaat caatcaatgt aagtgtatta actaatgcat atgatacgta tgtacaaaat
     8701 tcatcaagca aaaacatggc aagactatct ttattatttg cctcatctat taacttgact
     8761 aaagaaaaat ctttacagtt aattaatggg gagttaggat aaatatggct caatgattca
     8821 attcttttaa ttagattact gacacttgta taataatcta aacatccatt gttaccacaa
     8881 ctgcacaatg gtccactaaa attaattgat gtatgtccta tttctccact ttgtcctagg
     8941 ttgccagtat gaagtttatt ttctagaact agaccagctc caataccatt cattatgtgt
     9001 aggtatatat aatttgatat atttttacct aaaccataca ttttttctgc taatgcaccc
     9061 gaattagcat catttatcaa aaatattgga aaatcagaca tttcttttat aaaatctact
     9121 atatttaggt ttgatacatt tccaaagtct gggggattta gtatggtacc atttatatca
     9181 tctactggac ctattgaaga aataccaatt ccaattatat ctctcttgtt gattttcatt
     9241 attttagtaa acattctttt tattatatct tttaaaacat catttgatag acatttaggg
     9301 taagtttcag aaagttgttt tacaatatta ccttttaaat ctgaaataat taccttacat
     9361 atatttcttc ttattaggat tccacaaata caaggcgata tattagaaat atctaataaa
     9421 ataggtttac gacctgatga atgtgcattt aaagttgatg actctatttc cctaatcata
     9481 ttttcattaa taagttcagt aactagattt gataaagtca ttttagtaag tcctgtagac
     9541 ttagctaaat caactctgga cataggtgta tttgtagaaa gtaatttcaa tattattaac
     9601 ttatttttaa tttttacatc tttgatgtta gaaccttgtt tgttactcat cataaatctc
     9661 cttaaataaa gttaagtgtt ttatttattt tcaagtatac tatcacctta taatagagta
     9721 acattttttt acttgaaaat atcattaatt agaatatatc acattatata tcataaaaca
     9781 tataaaaaaa tgttagaatt aaattaaaag tgaaaagtag attgatatat aaatagtaaa
     9841 tacttaatta aaaaaagata atgatagcaa taattattgt taccaagaat ctactacttc
     9901 aagtgttacc ttaggtattt aagttgatag tggtttgcac gtaataaact tgatgtaata
     9961 tatattctta ataatatcga taaaagattt acttaaaaat aatatagatt atgtaaacat
    10021 ataatctatt tattgtatac aataaaattt cattttgtaa aacagtgaat taaatatgta
    10081 atatagatat cgatttttat tacaaattgt tactaaatat aggtacatta gaacaaaaat
    10141 ctagtaaatt gtaactaaaa tgtaaatttt tcctttaatc tatacaatat tatggaaact
    10201 ttttgtaatt tatgatataa taacaaatat ataattatta aattataata aatgactata
    10261 gcgtagatgt aatactatgt tatagtcatt tttgaattaa acttgatata tcaaggggga
    10321 agaatatgag aacaattaga agagttttag cattaggttt aacattagct atatttctaa
    10381 taaatgtacc taatgtagat gcattaacat cagatacaat taaaggtaac aatatatatg
    10441 agacggctgg attaatagca gataaaaaga gttatgatac agcaattatg gtaaatatgg
    10501 ataattctat agcagatggt ctttcagcaa gtggtcttgc aggtgctgtt gatgctccaa
    10561 ttttgcttgc acaaaaaaat aagataccaa atgaaacaaa acaaagatta aaaaatgtta
    10621 aaaagatata cataataggt aaagaattat caataagtaa gtcagtagaa actgaactaa
    10681 aaaacacagg ggcacaggta actagattgg gtggagatga cagaataaag actagttata
    10741 gtgttgctaa agaggtaaat ggtatcaaaa aagttgatga agtgatatta actaatgcat
    10801 ataaaggaga agcagataca ataagtgctg cacccgtatc agtaagagat atagctccta
    10861 tagtacttac agatggaaag agtgtgcctt tttcaacaag caatgtaaaa acatatgcag
    10921 taggtgggag tatatcaatg agcactagtt tagttaataa aactaatgct aaaagacttg
    10981 gtggttcaga tagatatgat acaaataaga aagttataaa agagttttat ccagatgcat
    11041 cagaatttta tttgagtgat ggatatgatt tagtaaatgc acttacaggt tctacaattg
    11101 ctaaggaaaa tccaattgta ttggtgtcag aaagtagtga taagtctata ttagcaggag
    11161 cagataagat tactagatta ggttcaataa gtgacagtgt gtataataag tgtgtttctg
    11221 ctgcacaaaa taatggcgat tcgtctacaa aaggtgagtc acctatgaaa aatgagacaa
    11281 gcatattagg tcagccaaca gctagtttag aagcgtgctt aaaatgggca aaatctaaaa
    11341 aagctaatga tttatttata gagttaatac caatattata tgatactgct gttcaggaag
    11401 gtgttaatcc tgttttggca gtagctcaat ctgcaaaaga aactggtttt tgtaattttg
    11461 gtggagtatt agatgcatca tttaagaatc cttgtggact taaaacttct gtaggtggtt
    11521 ctgatactga taagaatgcc cattcaagat ttgatacttg ggaagaagga atattagctc
    11581 aaattcaaca tttatgctta tatgcaggac aagatggtta cccactttca aatccagtag
    11641 accctagaca tgaaaaatct ttatttggta aggctaaaac agttgaaagt ttatctaata
    11701 attgggctgg aggtcaatat ggacaagatt tagtaagaat gatgggagaa attgaggcaa
    11761 caaaataatt gaagattatc aaatatccaa taaataagga ttgattatta tgaaaaagag
    11821 taatattttt agtatcatat ttcctacaat cataatggtg ctaatgtttt taacttttgc
    11881 tacagatata tttaatattc cagatattca atctaaaagt atttttgttg tgggaatggt
    11941 tttgattttt ccaatttctt ttttaataca agggataatt tgtgtattaa ataagactaa
    12001 ttggatttta tctcttattg tgtctttagt aacttatata gtattaatgt atatattttt
    12061 aaatgattct gcatatatat atgttttact ttatacagct ttttatatga ttggacatat
    12121 aacagctaaa ctctattaca aaattaagac ttttaaaaat taaattgaag tatataaaag
    12181 taacattaag agaagtatcc ttaatgattt tctaaaatca gaaataggtg cttctctttt
    12241 ttgtaaaaat aaaagtatgt aaataaaatt taaccattat aattttcttt taacaaatgt
    12301 atacaataaa cttgttgtat aattaagata gataaaataa agggagggat aatatgaatt
    12361 taatagataa tcatattcat actaattttt caagtgatgg aaaggattcc atggaagata
    12421 caataaaaaa agctataagt attggtgtta gatatttgac ttttacagac catttagaac
    12481 atgatgaaga tagaggattt tgcataaatt ataatgacta tgttccagta tttaataagt
    12541 ttaaagaaaa atataaaaag gatatagagt tattactagg tgttgaagta ggttacagaa
    12601 aacatctaaa aaatgaaata gaagaaataa taaattctaa tccttttgat tttgtattgt
    12661 gctcaactca tacaattgac aatgtacctg ttccatcaaa ggcgtatttt aaaggtctta
    12721 gcaaagaaga tgcgtattat aaatatttta atagcatact tgaaactaat cgtgaatttg
    12781 gagattacaa tatatatggt catttggatt atatatctag gtatggaata tattcagata
    12841 atagagttat atacaatgac tttaaagatg ttattgatga ggtcttaaag tcaataatca
    12901 ataatgggag tggtatagag ctaaatactt ctggttatag atatggatta aatgctatac
    12961 atccaaatga agatattcta aaaagatata aagaacttgg aggatttata gttactgtag
    13021 gttctgattc tcatagggta gaagatatat gtaaagattt tgatgtggcc tatgatatgc
    13081 ttaagtatct tgactttaaa tatgtatctt tatttaaaga aagagaaact tattttgtaa
    13141 atataaaaaa agttaaatct aacaatatag cttagagtat gttttgaagt aaattaatgg
    13201 atagttaaaa agttaatagt tataatattg tatcctctag aaaaatacta gaggttttta
    13261 ttttgctaaa ataactaaat
//
LOCUS       DACTGW010000095        13160 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.095, whole genome shotgun sequence.
ACCESSION   DACTGW010000095 DACTGW010000000
VERSION     DACTGW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.095"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            201..512
                     /locus_tag="I9573_002103"
     CDS             201..512
                     /locus_tag="I9573_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796723.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VRKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            514..2439
                     /locus_tag="I9573_002104"
     CDS             514..2439
                     /locus_tag="I9573_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAT4796724.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            2442..2933
                     /locus_tag="I9573_002105"
     CDS             2442..2933
                     /locus_tag="I9573_002105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAT4796725.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            2985..3548
                     /locus_tag="I9573_002106"
     CDS             2985..3548
                     /locus_tag="I9573_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796726.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            3563..4540
                     /locus_tag="I9573_002107"
     CDS             3563..4540
                     /locus_tag="I9573_002107"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HAT4796727.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            4527..4850
                     /locus_tag="I9573_002108"
     CDS             4527..4850
                     /locus_tag="I9573_002108"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAT4796728.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            4967..6745
                     /locus_tag="I9573_002109"
     CDS             4967..6745
                     /locus_tag="I9573_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase alpha chain"
                     /protein_id="HAT4796729.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            6742..8115
                     /locus_tag="I9573_002110"
     CDS             6742..8115
                     /locus_tag="I9573_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAT4796730.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            8121..8789
                     /locus_tag="I9573_002111"
     CDS             8121..8789
                     /locus_tag="I9573_002111"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAT4796731.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            9066..10115
                     /locus_tag="I9573_002112"
     CDS             9066..10115
                     /locus_tag="I9573_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAT4796732.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTYNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            10211..11200
                     /locus_tag="I9573_002113"
     CDS             10211..11200
                     /locus_tag="I9573_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT4796733.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            11205..12923
                     /locus_tag="I9573_002114"
     CDS             11205..12923
                     /locus_tag="I9573_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HAT4796734.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(13013..>13160)
                     /locus_tag="I9573_002115"
     CDS             complement(13013..>13160)
                     /locus_tag="I9573_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT4796735.1"
                     /translation="VEENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKK
                     KINK"
BASE COUNT         4965 a         1688 c         2497 g         4010 t
ORIGIN      
        1 gtctaaaagt aagtcaaaaa aggatgtaaa tctaggcaag tactattcta aaatatgttt
       61 tattaagaaa taaacgttga aaaatattaa ttattatata aaaatgctgt ttatacttta
      121 acaatgaggt agtaatctaa ttagaataaa aaaatctaat tggttatata catagtaaaa
      181 aggtaagaga ggtgatttgg atggcttttg aaataataaa aagcattgtt gaggcagagc
      241 agacagcaga cagtatcaaa gtaaaagctg ttactgatgc agagtctatc agagctgatg
      301 ctgtaaacaa atgtgaaagc atatttgctg atgtaagaaa acaagcaaag cttatggaag
      361 aaactcttat tgagaaggca gtcaccgaca gtagagcaga ggttgataaa atcttagcta
      421 atgctaaaag tgaatgtctg aaaattgaaa aaactgctga agaaagaaaa agtaaggcta
      481 ttgaagcggt tattggaaag gtagtgagat aatatggcaa tagttaatat gaacaaaata
      541 tctattgttg gcttagaagc tcaaaaaagc cagattttaa agctacttat gaaccgtggg
      601 tttgttcaga ttgatgatag tgcctttctt actgaggagg aggaattaaa aaattatctt
      661 gtaaaagata gtgaagaatc tgaagttata atgctagagc agaaaatgtc tcatgtaaac
      721 caagcaatta atattgtttc ttcactagtg aaacaaaaaa aatccctact tgcaccaaaa
      781 agagagttta acaaaattga tagtgaaaaa gaagggcaag tttatgctga agctctggag
      841 ttaaacaatt tgcacaagga gataggtaca ataaggtcta acataaactt acttaacaac
      901 aataaaagta tgttagaacc atgggtgaac tttgatattc ctcttgaaaa tatggaaact
      961 aagtatacaa aaactatact tggaactgtg cccataagcg caaatattga tgctttaaga
     1021 gtaaaattag aagatgaaat accagaaagt gttattggga ttgtcaattc tgacaagtta
     1081 atgaactatg tttatttaat tactttaaaa gaaacttttg atgatgtttt agaagtttta
     1141 aaagaattta atttttcagt ggtttcatta ccaaatgaag aaggtacacc gttacaagct
     1201 atcaagaaat acaatgaaat gataattagg caggaaaacg tttctaagga caaaacagag
     1261 gaaattaaaa gacatgcaga tagtatacac atgcttgaaa acctatatga tttttacact
     1321 atagaaagag accaaaaaaa gattatagag agacttgtca aaacaaaaac aacattctgt
     1381 cttaatggtt ggttaccaag caaaagggca gatgaattaa ttaaagaaat tacagaaaag
     1441 tttgattgtt gtgttcagac tgaagaagga agtaaagaag aaggcttccc aattctgctt
     1501 gaaaacaata aattagtaac accttttgaa agtgtaacta acatgtacag ctatccaagt
     1561 acaaaagata ttgacccaaa tacaatttta actttcttct ttgttgtatt ctttggaatg
     1621 atgctaagtg acgccgcata tgggttaata attgcagtag tctgtggatt tgttgtatat
     1681 aaattaaaga tacaaaaagg tgaaggaaat ttaattaaac ttattggtat ctgtggagtt
     1741 tcaactactg tatggggtct tatattcggg ggtattttgg gggatttaat tcctataaaa
     1801 gcattaatta atcctttaga ggatgttatg acacttatgg gaatgtcgtt actgttcggt
     1861 attatccata tatatgtagg tttgggaata aaagcatata cattaataag aagtggaaag
     1921 gtaatagatg caatctttga cataggtttt tggtatttgt gtataacagg tgtatgtcta
     1981 cttataatac cttttgttgc aggggacata ggtgtatttt cagaagtagg aaaatattta
     2041 gcaattatag gtgctatagg tcttgtactt actcaaggaa gaagctttaa aggtattcct
     2101 gttaagcttt ttaaaggatt ttcaagtctc tatggcataa caagttattt tgctgatata
     2161 ttatcttata caagaattat ggcgctttgt ttaacaacag gagttatagc acaagttatc
     2221 aaccttttag gggctatagc tggtccgata cttgcagttg taatcggtgt tgtaggacac
     2281 acaataaacc ttcttataaa tgcactaggt gcatatgttc atacaagtag gttacagtat
     2341 gtagaatttt tcaataagtt ttatgaaggc ggaggagttc cttttgttcc gtttaagtat
     2401 aaaacaaaat atacaagcat taataaaaag gagatgtaaa tatggaattt ttaaacgcgt
     2461 taggaaatgg tcaatttttg gcaatatcag gagctgctat agcagcatta tttgcaggta
     2521 tgggttctgc taaaggtact tcaatagcag gtcaagccgc agcaggtgtt gtaacagaag
     2581 accctagtaa attcggtcaa cttctagttt tacaacttct tcctggtaca caaggtctgt
     2641 atggatttat catagctttc cttattttat caaaagcagg gattattggt ggagatgcaa
     2701 taccaacatc agctcaaggt ttacaacttc ttatggcagg acttccaatt ggtttagttg
     2761 gtttagtatc aggtatagca caaggtaaag cagcagcagc aggagttgga atagttgcaa
     2821 aaagacctga agaacttggt aaagctataa catttgcagc aatagttgaa acatatgctc
     2881 ttttaggact acttgtttct ttcttagctt acaatggaat tgcaattggt taattataaa
     2941 ttataattca taagtaaaaa aaatgaaaag gaggaagcac tcctatgggt aatgaacaaa
     3001 aaatgattga cagaattata gctgatgcca aacaagaagc acaagaaata ttggataaag
     3061 ctaaaagtga agcagattta aaagtaaatt cagctaatga aaaagctgaa aaggaaatgg
     3121 cttcctatac aaagcttgca gaggcagaag cagaaaaagc ggcttctaag gaaatatcag
     3181 gagcttatat ggaggctaaa aagcaaatat tatcaaaaaa acaagagatt ttagaagagg
     3241 taattttaga agctaaaaac aaacttttaa atcttaaaga taatgaatat gaagagatta
     3301 tactaaacat gattgaaaaa tcaaattgta cagatgattc agagatagta ttatctaaaa
     3361 aagataaaaa aacattaaaa gatgtgctat caaaaaaagg cattaaagtg tctgatgaaa
     3421 ctagagatat tactggaggt ttcatagtta aaaaaggtga cattgagtac aattattcat
     3481 ttgaagccat aattgcagta gagcatgaat atattgaaca aattgcagca gaaattttgt
     3541 ttaattaagg aggggttaaa aaatggcaga agaaaaaact tatccctatg ccgttgcacg
     3601 aataagagtg cttgaaaaac agcttcttaa cagacaaatg tttattcaaa tggcagaagc
     3661 caaaagccct gaagactctt taagaattat agctgaggca ggttatgctg atacatcaaa
     3721 taataatata cataattttg agaatatttt gactcaagaa ttatcaaaga catatgaaat
     3781 tcttaaaagt ctagctcctg aagaaaaatt tgttgatgta tttctttata aaaatgacta
     3841 tcacaattta aaagtgctga ttaaagaaga aatttctggt gtagatggtg agaaatattt
     3901 aattgatgga ggtacaattc ctcttattaa gcttagagaa tctttagcaa atcgtagttt
     3961 tagtgattta cctaagataa tgtcatcagc tatattagag gcatttgatg tttataataa
     4021 gacacaaaat ggccagatgg ttgatattgt tctagataaa gcaaccttta ccagtatgaa
     4081 agaaactgct aaagaaagcc aaaacaaatt tgtaatagat tatgttatga aagtttgcga
     4141 tttaacaaat ctaaagagtt ttataagagt caaaaatatg aagaaagatt ttgatatgtt
     4201 tatgaatgtg tttgtttcag gtggctcact tgataaggaa aaattcttag aagctttttc
     4261 ctcagatact ccagcatcat gttttaaatc tacttcttac tctgatgtct gtaaaaatgg
     4321 catggatagt ggttttactg tctttgaaaa actttgtgat gactatctta tggaatatgt
     4381 tagaggagca aaatttaagc ctcttacatt agaaccacta attgcatatt tatatgctaa
     4441 agaatcagag ataaaaacaa ttagaattat tttgactagt aagcttaata atatagacgc
     4501 agatactata aaagaaaggc taagggatgc ttatgtataa ggtaggagta gttggagata
     4561 aagatagcat catgggattt ttagctcttg gaatagatat cttcccagcc tatgatagtg
     4621 acgaaattaa aaaaagtata cataagttgg ttgaagatga atatgcgata atttatatta
     4681 cagaacaggc atcattgctt gcaaaagaat caatagcaaa atacaaagat taccaacttc
     4741 ctgccattat agttattcca ggtattggag gaagtatggg attaggtatg aatgaagtaa
     4801 gagaatctag taaacgtgct ataggtgcag atatattatt taaagaatag atgaaagttg
     4861 aataaaagac aatctcaaat tttattggtg ctaaagatat gtgctaataa aatataggag
     4921 ggaatattta aattttgata aagtatatga ttgcaggtga gagaatatga aaacaggcac
     4981 aattgtaaag gtgtcaggac cgcttgttgt agcggagggc atgagagatg cgaatatgtt
     5041 tgacgtcgta agagtttcag ataaacatct tataggcgag attatagaaa tgcatggaga
     5101 taaagcttct atacaggttt atgaggaaac ttccggtcta ggaccagggg aagaagtagt
     5161 ttctcttgga atgcctatga gtgttgaact tggaccaggt cttatttcaa ctatttatga
     5221 tggtattcaa cgtccacttg aaaaaatgta tgatatttca ggtactaata taactaaagg
     5281 tgttgaagta tcttcacttg atagaacaac taagtgggaa tttaaaccta aaaaatctgt
     5341 tggtgataag gttgtagcag gagatataat tggtggcgtt caagaaacag cagtagtaga
     5401 atgtaaaatt atggttcctt atggagttaa gggtactata aaagaaatat attctggtga
     5461 atttactgtt gaagatacag tttgtgttat tacagatgaa aaaggtaatg atatacctgt
     5521 aactatgatg caaaaatggc cagttagaaa agaaagacca tatagagaaa agaaaacacc
     5581 agattcactt cttgttacag gtcaaagagt tatagataca tttttcccaa tcacaaaagg
     5641 tggggtagca gctataccgg gaccttttgg aagtggaaaa actgttacac agcatcagct
     5701 tgctaaatgg gcagatgcag atatagttgt atatataggc tgtggcgagc gtggaaatga
     5761 aatgacagat gttcttcttg aatttcctga attaaaagac ccaagaacag gcgagtcact
     5821 tatgcaaaga actgtgctta tagcaaatac atcagatatg ccagttgctg cacgtgaagc
     5881 ttctatatac actggtatta caatagctga atattttaga gatatgggat atagtgttgc
     5941 acttatggca gactctacat caagatgggc tgaggctctt agagagatga gtggtcgttt
     6001 agaggagatg cctggtgaag aaggttatcc tgcatactta ggttcacgtc ttgctcaatt
     6061 ctatgagaga gcaggaaagg taaattgtct aggtatggat gaaagagaag gaacacttac
     6121 agcaattggt gcagtttctc ctcctggtgg tgatacatca gaacctgtca gccaagcaac
     6181 gcttcgtata gtaaaagtat tctggggtct tgatgcatct ttagcatata aacgtcattt
     6241 cccagcaatt aactggttga ctagttattc actctatcag gaaaaagttg atgtatgggc
     6301 agataaaaat gtaaatgata atttctcagc tcaaagagct caagctatga aacttttaca
     6361 agttgaatct gaacttcaag aaatagttca attggttggg gttgattcac tttcagatgg
     6421 agatagattg atactagagg ttactcgttc tataagagaa gacttccttc atcaaaactc
     6481 tttccatgaa gttgatactt atacatcact tcataaacaa tatagaatga tgggacttat
     6541 attaaaattc tataagtcag cacaagatgc tatcaagcaa aatgttacta taaacgatat
     6601 tatcaagctt tcagttcttg aaaagattgg tcgtgctaag tatgtagaag aaggcgatat
     6661 agatgcagct tatgattcaa ttgaagatga aatagacaac gagcttgcag accttattaa
     6721 aaagaggggg gctgaagaac tatgataaag gaatataagt caatacaaga ggtagccggt
     6781 cctcttatgt taataaacgg cgtagaaggt gtaaaatttg atgaacttgg agaaattgaa
     6841 ctttcaaatg gagaaattcg ccgttgcaaa gttctagagg taaatggaga tactgcactt
     6901 gttcagcttt tcgaaagctc aacaggtata aaccttgctg aaagtaaagt tcgtttttta
     6961 ggaaagagtc ttgactttgg agtttcacca gatatattag gccgtgtttt tagtggaatg
     7021 ggtagaccta ttgatggagg tccagaaatt ataccagata aaagacttga tataaatgga
     7081 gctccaataa atccagcagc acgtgattat ccggccgaat ttatacaaac tggagtctct
     7141 gctatagatg gacttaatac attggttaga gggcaaaaac ttcctatatt ctcagggtct
     7201 ggtttgccac atgccaatct tgcagttcag attgcaagac aggcaaaagt acgtggaact
     7261 gattctaagt tcgccgttgt atttgctgca gttggtataa cttttgaaga tgctgaattt
     7321 tttataagcg attttaaagc aactggtgca atcgaccgtt ctgtagtgtt tgtaaacctt
     7381 gcaaatgacc cagctgtaga gcgtgtatct acacctagaa tggcacttac agcagcagaa
     7441 taccttgctt ttgagcaaga tatgcatgta cttgttataa taacggatat tacaaactat
     7501 gcagaggcat tgcgtgaagt ttcagcagct aaaaaagaag ttccaggacg tcgtggatat
     7561 ccaggatatc tttatactga ccttgctact atgtatgaaa gagcaggaaa aatgaaaggg
     7621 catgaagggt ctataactat gattccaata cttacaatgc ctgaggatga taagacacat
     7681 ccaattcctg accttacagg atacattaca gaagggcaaa ttatacttag ccgtgaactt
     7741 tataaaaaag atatacagcc tcctatagat gttttaccat cactttctcg tctaaaagat
     7801 aaaggtattg gtaaaggtaa gactcgtgaa gaccatgcag atacaatgaa ccagcttttt
     7861 gcagcttatt caagaggtaa ggatgcaaaa gaacttatgg ctatacttgg tgaatctgca
     7921 ctttctgaga ttgatttaat gtatgctaag tttgcagatg aatttgagaa agaatatgtt
     7981 tcacaaggat ttagaactga tagaactatt gagcagacat tagaaatcgg atggaagctt
     8041 cttagtatgt taccaaaaag tgaacttaaa cgtatccgtg atgaatattt tgaaaaatat
     8101 atgcctaagg agtgatatct gtggctagat taaatataaa tccaacacgt atggaaatga
     8161 ctagacttaa gaaactcctt aaaactgcta caagaggtca taagcttctt aaagataaac
     8221 ttgatgaact tatgaaacaa tttcttgaga ttgtaagaga aaataagcga ttgcgtgaag
     8281 aagcagaaaa tgctcttgat actgcttata aaaactttat aatcgcaaga gcagttatga
     8341 gtcaggagta tctgggttca gcacttatga tgccaaagca atcagtttca gtagatgttt
     8401 ctacgagaaa tataatgagt gttgatgttc ctgtgtttga ctttaaaact gaaaacaatc
     8461 aaagtgatat ttatccttat ggtcttgctt ttacatcagg tgaattagat tctgctatgg
     8521 aagctttctc agatgctatg caaccacttt taagacttgc agaaagtgaa aaaagtgcac
     8581 agcttcttgc acaagagata gaaaagacac gtagacgtgt taatgctctt gaaaatgtaa
     8641 tgataccaaa ttacattgag actattaagt atatagctat gaagcttgaa gaaaatgaga
     8701 gagcaagtac tactagactt atgaaggtta aggatatggt gcttaaaaaa gcacttgaag
     8761 aaaagaaaaa aaatgatttg gttgtataat taaatactta taaaaataag tctaacattc
     8821 tactttatag aggttagact tattttttat gtttaaatta aaatgtagat actataaaaa
     8881 ttaatatatt gtttaaaaac agactgtatt taactaaaaa taatatattt aacaaaaatt
     8941 tctaaaaatt aacgaactgt taatttacac ttaactttat attaacaaat taggtgtatt
     9001 atataaacat aagcaaaaac aataaaaaac aaataaaaaa aggaaaaaac acaaaggggg
     9061 aagatatgaa agaatttata tttacttgtc tgtgcattgt agtgattaca tttatgacaa
     9121 gttgcatatc aacaacacca caaactactc aaggaaaagg aaataataaa cttacaatat
     9181 atgcagcact agaggaagag cagatagctt attatatgga aggttttaga gaagagtatc
     9241 ccaatataga tgtagagata gttatggatt ctcatggtgt aatagcttca aaactattag
     9301 cagaaaaaga taatccacaa gcagatatta tatggggatt atcagcaatg aacatgatag
     9361 agctaaaaaa agatggtgca cttgaagcat ataagcctaa aaattatgat aaagttgata
     9421 aaaaatttaa agatactgaa gatgatgtat cttgggtagg aatgagtgtc ccagagacag
     9481 caatagtagt taattataag gaacttaaga aactaggtat agatattcca aaaagttatg
     9541 aagatttaat aaaaccagaa tataaaggac ttataacaat gccaaatcca gcatcatctg
     9601 gaacggggta tcttacagta tcagggctta ttcaaatgat gggtgaaaaa gatgcttaca
     9661 attacatgga taaattacat aaaaatatag ctacatatac acactcaggt tcaaaacctg
     9721 cgaagttagc tggctctgga gagtatccaa taggtattac acttggatat agagctacac
     9781 aacaattatc tagtggagaa cctatagagg ttgtatttcc aaaggaaggt tcaggttggg
     9841 acttagaagc aaatgcctta gttaaaaaag ataatataaa aaaagaagca aagctatttt
     9901 tagattgggc aataagtgat tcagctatgg atagatatgc acatgaagta gcagttactt
     9961 ccataaagac atataatcca gttccaaaag gttattctaa acaaccattt gaacagctta
    10021 taaaagaagt tgatttaaac tctgctgcca aaaacagaag tacagtactt aaaacttggg
    10081 aatcaaaata cggttcaaaa tctgaaagta agtaaggttt aaattaataa tttagatgaa
    10141 agaattttag aagaaaaagt aggatataaa atttaaaata aaaaaataac tatataagga
    10201 gagataagat atgagttact taaaaataaa taatgtgttt aaaagttatg accaaaaaag
    10261 ggttttaaat aatattagtt tggatattga agaaggggaa tttctatgtt tactcggacc
    10321 tagtggatgt ggtaaaacta cattacttag aataatagca ggtcttgaag atgtaaatag
    10381 tggaactata attctccagg ataaagatat tacaaattta gagccatcaa agagaggttt
    10441 tggtatagta tttcaatcat atgcattatt tccgaatatg actgcttata acaatatagc
    10501 ttttccacta aaagagagaa aagtttcaaa agaaaaaata gataataaag ttaaggaagt
    10561 acttgaaaca gttggattga ctaatgaagc tcataaatat cctaaagcat tatcaggtgg
    10621 gcaacaacag aggattgcaa tagcaagagc attagctctt gaaccaaagt ttttactttt
    10681 agatgaacca atgtccgcat tagatgcaaa ggttagacac aagcttagaa tggacattaa
    10741 aagattacaa aaagaactta atataacgac aataatggta acacatgacc aagaagaagc
    10801 tataactatg gcagataaaa tagctattct aaatggagga gatataatgc aaatagggac
    10861 tccagaagaa atataccaaa atcctcaaaa cctatttaca gctcaattta taggagatac
    10921 aaactgtttt gataatggag attctatatt aactgtaaga ccagaatatg ttcaaataga
    10981 gaaatcgaca aaagaaaact accaaggtat aatatcaaat atagagttta gagggaattt
    11041 attgagagtt gaaataaaag ataaattaaa tgaaaatttc ataataagtg atgtttctat
    11101 aaaagagtgg gttaatttaa atttagtaga aggtgacctt gtaaaaataa gtatagacga
    11161 aaaatactat ttaaagtatc caaaagttaa aggagcataa gtgtatgaat aataatgcta
    11221 taagttctaa aagtataagt cttaaatcaa aaatgaattt agatataaaa aaatgtatgc
    11281 ttacagtatt agttatgtct ttagtaatat ttttattagc acctataata tatctatttg
    11341 tacaggcttt tttagataaa gatggagtct ttgtaggatt acaaaacttt caagaatact
    11401 tggcttctga atctctatta aattctctaa aaaatacagt atttgtatca gttatatcaa
    11461 ctgtaatatc actaacactt gcattagtat atgcatatgc attaactaga acaaagatta
    11521 aatttaaagg tttttttaaa tcattagcta tgtttcctat gtttgctcca acaatgatgc
    11581 atgcaatagg tctagtatat ttatttggaa atcaaggttt ggtaactaat ttacttggag
    11641 tgcatgttcc aatatatgga gcagtgggaa taatattggc acaaattata tatgtatttc
    11701 ctcaatcctt tcaaatactt tatatatctc taagttcaac cgattataga ctatatgaag
    11761 ctagtaatac tatggggata agcaagataa gagaatttat aagcataaca atcccaagta
    11821 taaaatactc tttaataagt tctgcattcg tatcatttac attatgtttt acagattttg
    11881 gagctccaaa ggtgatagga ggagagttta atgtattatc aatagatgta tataagcaaa
    11941 ttatagggca acagaatatg ccaatgggag caactgtatc aatattactt ttaatacctg
    12001 caataatatc gtttatagta actaaaaaaa ttgaaaaaaa tcaaaactca tatataactt
    12061 caaagtcaat tccatataag gttaaaacta ataagttaag agatagtatt ttaggagggg
    12121 ttgtaggaat tattgcattt ggtataatag ctgtaatgtt aactgtaatc ttatcctcat
    12181 taatatcagt ttggccatat gatttaagac ttacactgga acacttttct atgaaaaaca
    12241 gtattgatgg aattaaacca tttataaatt caataaaaat gtcactattg acagctattc
    12301 tagggacgag ctttgttttc ttatttgcat atttaaatga aaaaacagaa aaagctccat
    12361 atttaaaatc atttgggtat tttttaagta cattgcctat ggcattgcca ggattagttt
    12421 taggtatagc ttatgtatta ttttttaata aattagagtt taattttatg gatacaatat
    12481 acattaaaaa tttgtttaat ggattatatg gaacattact tataatggtt atatgtaata
    12541 tagttcattt cttttcagta gcatttataa cagctactac tgctattaag aaagtagata
    12601 aagaattgga catggtagct aaatcaatgg gaataaacag cgcatcaatt cttaagaacg
    12661 taactatacc actttgtttg ccagcagttt tagagaactt tatgtacttt tttctaaact
    12721 ctatggtgac aatatcagca ctagttttta tatatactgc aagttcaaaa gtagctgcaa
    12781 tatcaattgt acaactagat gataagggga gtactgcaca ggcagcagca ctagctgtgt
    12841 taatattagt cacaaatata ttagtgaaag taatatatga atttataaag aatcgtttag
    12901 aaaaaagaac aaactttaat taaataattt atcttataaa aacacaaatt taaaaagtct
    12961 attcttatga tttattattt aaagaataga ctttttaaat ttattattga tattatttat
    13021 taattttctt tttgttatca tacatttgag aatctgcacg acgatatgta tcaaataaag
    13081 aaacatctac tttttcatca aaaatagcat atccaataga cattgataat tttaattcat
    13141 tagttgagtt ttcttcaact
//
LOCUS       DACTGW010000096        13122 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.096, whole genome shotgun sequence.
ACCESSION   DACTGW010000096 DACTGW010000000
VERSION     DACTGW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.096"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(46..249)
                     /locus_tag="I9573_002116"
     CDS             complement(46..249)
                     /locus_tag="I9573_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HAT4796736.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            540..1304
                     /locus_tag="I9573_002117"
     CDS             540..1304
                     /locus_tag="I9573_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAT4796737.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKYDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            1693..3006
                     /gene="prdC"
                     /locus_tag="I9573_002118"
     CDS             1693..3006
                     /gene="prdC"
                     /locus_tag="I9573_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HAT4796738.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            3175..3337
                     /locus_tag="I9573_002119"
                     /pseudo
     CDS             3175..3337
                     /locus_tag="I9573_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3807..5567
                     /gene="prdR"
                     /locus_tag="I9573_002120"
     CDS             3807..5567
                     /gene="prdR"
                     /locus_tag="I9573_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HAT4796739.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            5996..7876
                     /gene="prdA"
                     /locus_tag="I9573_002121"
     CDS             5996..7876
                     /gene="prdA"
                     /locus_tag="I9573_002121"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HAT4796740.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            7957..8241
                     /locus_tag="I9573_002122"
     CDS             7957..8241
                     /locus_tag="I9573_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796741.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            8279..9004
                     /gene="prdB"
                     /locus_tag="I9573_002123"
     CDS             8279..9004
                     /gene="prdB"
                     /locus_tag="I9573_002123"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:8729..8731,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HAT4796742.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            9084..9842
                     /gene="prdD"
                     /locus_tag="I9573_002124"
     CDS             9084..9842
                     /gene="prdD"
                     /locus_tag="I9573_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HAT4796743.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            9857..10324
                     /locus_tag="I9573_002125"
     CDS             9857..10324
                     /locus_tag="I9573_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HAT4796744.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            10342..10824
                     /locus_tag="I9573_002126"
     CDS             10342..10824
                     /locus_tag="I9573_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HAT4796745.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPAVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            10844..11851
                     /locus_tag="I9573_002127"
     CDS             10844..11851
                     /locus_tag="I9573_002127"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HAT4796746.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            11971..12852
                     /locus_tag="I9573_002128"
     CDS             11971..12852
                     /locus_tag="I9573_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAT4796747.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         5175 a         1533 c         2526 g         3888 t
ORIGIN      
        1 gctaattagc agccctgttg atacccaggt gtcaaaattt actatttatt ttccagccat
       61 ttgtctttca gccatttcaa ctaatttctt agtcatatat ccaccaacat atccattttc
      121 tctagctgtt aagttacctt tatctatgtt ttcatagtta gataaaccta tttcgttagc
      181 tatttctaat ttcatttggt ttaaagcagc ttttgcttct ggaactactg ttctgtttga
      241 atttgacata gtaaatttcc tcctcaaata attagtttat gatgtctaac aacactttta
      301 tttatgtgat gttaatacta atttctccaa aataaaaata atatatacag aaaaataaag
      361 gtaaaaatgt aaaaaatata aaaaaattaa ataaatgagt attttagaga gtttaattag
      421 gtcaatatag gttttatttt tttagttatt gtcttataaa ttaaatatta cagtactttt
      481 ttgagctgaa aaaaaataaa ttttaaatat agaagaaatt atttcaaagg gggaatagta
      541 tgaaaagaga agttaaaagg actaaagctt tgatatatag tgttttattt ttttcattat
      601 tattagtgtt aatatttgtt tttgtttatg gaataaaata catatgttct aaatacgaca
      661 attcaaaaat gcctatatat aaagttgata caaatgagaa aaaaatagcc ttgagttttg
      721 atgtagcatg gggaactgat aatatggatg atattttaaa aattttagat aaacataatg
      781 taaaagctac cttcttttta gttggtagtt gggtggatga taatgaagaa attgtaaaag
      841 ctatagatga aaaaggacat gaaattggaa atcattcaaa cactcatgcc aatttgaagg
      901 aaatatcaaa agaagacata acaaaagaga tagaaactac atcagaaaaa atatataata
      961 taacaggaaa aaagactaat ttatttagac ctccttttgg ggacgtaaac aataaagcta
     1021 tggacatttg tagtgattta ggttataaag taattaagtg ggatgtagat tctatagatt
     1081 ggaaggagct tggaccaaat catgtaattg agaaagtaat aaaagaatct caacctggtt
     1141 caatagtttt attccatgct aatataaatg atgtagataa ctatttggat actataataa
     1201 gtcgactaaa aaaagatgga tacagtctag tcaaaatatc tgatttgttg tataaagata
     1261 attatgtagt tgattccaat ggagtgcaaa aaactaagaa ttaataagta ataaatgtat
     1321 attttaagta aagttgtgat aaattatata gtatatgtta gaaaagtgtc gataaaaaga
     1381 cggtgtctat tttttgacac tttttataca tgtatgcaat atcaataatt atagattaaa
     1441 ttgattaaaa aagtgtctaa taattgactt ttttttgatg gatttaaaaa tttagaagaa
     1501 ttaatgaatg ctgtaagaca gtatttccaa agggtataag tttgtttaaa agttggcata
     1561 gaaattgctt tgttaataag cgatatgttc tatagaaata aacaatatgt taagacctta
     1621 taatagaaaa atagcattaa cattatttga acagcttata aaaaaattaa tatacttaag
     1681 ggggcgtact agttgaacaa actatattct attttattaa aacaacacgt tggtggtcca
     1741 gataagccag tagtaagtgt tggagatgta gtaaaaaaag gaactctaat cgcagaacca
     1801 actggattag gggcaaatat ttatgccagt gttagtggaa aaataagtga aataaatgac
     1861 caagcgatag taattgaagc tgatgaagta caagaagata cttttgaacc attaaaagga
     1921 gaaggtatac ttgacttaat taaagaagct ggagtagtag gaatgggtgg agcaggattc
     1981 cctactcatg taaaattaaa catagattta aatggtggaa caatattagc aaatgcagct
     2041 gaatgtgaac ctttattagc tcataatata aaagaaatag aagaaagacc agaaatagtt
     2101 tatcaaggta taaaatatgc tatggaagtt actaatgctg gaaaaggtat gctagcaata
     2161 aaaagcaagc atccaaaagc aatagaagct ttcaaaaaag taataaagcc aggagacaac
     2221 atagaagttg cagaactagt tgatatgtac ccaatgggag aagagagagc aatagtaaga
     2281 gacgttctag gaaaattact tgagccaact caattacctt cagaggctaa tgcagtagta
     2341 ataaacgtag aaacactaac tagaatagta gaggctgttg agcaaaaaag accagttata
     2401 tctaaaaata taactgtagt tggtcaatta aacagtggaa aagagtctat agtatttgaa
     2461 gatgtgccaa ttggaacaac tgtcggagaa ttaattgaaa gagctggtgg aataaaaggt
     2521 gaatatggtg aaataatcct aggaggacct ttcactggta aagctactac tttagatgct
     2581 ccaataacta aaactagtgg tggtataata gttactatgc catttgttaa tgaaaagaga
     2641 aaaatgggtc ttttagtctg tgcatgtgga cctaatgaag agagaatgag agatatagca
     2701 actaaaatgg gagttactga tatagtatca gttcaaaaat gtaaacaagc tcaagaaatt
     2761 aaaggtgcat taaaatgtga aaatccagga cactgtccag gacaagctca aaaatgtatt
     2821 gaatttaaga aagctggagc agaagttata ttaataggaa actgtactga ctgtagtaac
     2881 acagtaatgg gttctgcacc aaaattaaag ttaggtgtat atcatattac tgaccatgta
     2941 atgagaacag ttaatcaccc attaataaga agaataaaag gtgacgtaag attaaaaata
     3001 aaataataga taaatagaag ttagacatct ttagaagtaa aacttaaaaa ttacttctaa
     3061 ggatgttttt taatgtattt tgagggcaat atatagcttt aaaagaaatc aaaaaagtgc
     3121 caaatgtaaa actaattaaa ccatatatat ttaagaaggg attgaattgg agttgtgaat
     3181 tatgttagta gaaaccattc taagtgtttg ttattggtac atttgatatt tgtatgtaaa
     3241 tacagaaaaa actttttgct cagatggata ctttgctttc agtgtaggaa atgtttctaa
     3301 agaaatgatt gaaaaataca tacaaagtaa gggataggtt tacatccacc aacctaaagg
     3361 tatagtggtt ttacgcctta atttataaaa agaaattaat ttatattatt attaagttta
     3421 aaataaaaag tctcaaattg atttataagt cattttggga cttttttatg taaatataat
     3481 atgcaaatat agtttgttta aaaatatcta ttttaattat attcttaaat ttatgattgg
     3541 atacaggcat tcattaattg tttgatgaat agaaaaatgt tttgaaggtg aaaatagtat
     3601 tgaaaaaata atatactaag ggtataattc atttgatttc atttgtataa atttaattat
     3661 aaattttaaa gtgattatat aaatttaaat gctaattact taataaattt taataataat
     3721 tatttaataa ataattatta taatatgcta gaataaatat atataaataa gttttgatga
     3781 ggtactacta taaagggggg gactttatgt tttcgatacc agaagtaaaa aaagtagaag
     3841 aggtaatgga tacaaaattt acaactatag acgaagacac aagaattgaa gatgcaataa
     3901 aagaaatgat aaaaagcaac acaaaaactc taatggtaat agattcatct gaccaattaa
     3961 aaggcattat atccatgacg gatatacata atctttatga gatgcataaa aagtatgaag
     4021 gtcaaccagt aaaacttata atgaagaagg atgtaatata tgtcaacgaa ggtttaacct
     4081 tagatgaatg tagagatata atgatattaa aaaatatagg gattcttcct gttcttagag
     4141 ataataaaat tattggagtt ctaaaacaag agcacattag ggactatcta tatatgcatt
     4201 tagaagatta tggattgact ttgaagtata ttataggtca aattaaagaa ggaatttgtg
     4261 ctataaataa cgaaggtgtt gttatcttgt ggaataagtt tatggaggaa aggtatgata
     4321 taaagtctga agatatagtt ggtcgaccga tgaatgaatt tttggaaaat actatttcag
     4381 aaaaagtact aaatagtaaa gttggaatga gtgacttgta ttttacagat aaaaaagaaa
     4441 atatgtatgc attagttcat gcaaatccta tattttataa agaagaattt atcggtgttg
     4501 tatgtacaga agttgatgtt acagaagcta agatattagc tcttgaactt gaaaaagtaa
     4561 atgatacatt aaaatatcta aagaacgaag tcaaaaattt atctaaaggt agttttgata
     4621 agattttagg taaaagttat aaattggaaa aatcgaaagc gattgcaaaa caagtggcta
     4681 gaacaaatag tagcatattt atttggggtg aaagtggtac gggtaaagaa gtatttgcaa
     4741 gagctattca tgattatagt gagagaaaag gtcaatttat acctgttaac tgtagtgcca
     4801 taccaaatga gttatttgaa agtgagtttt ttgggtatga atcaggtgca tttacaggag
     4861 caagtaaaaa gggtcgaata ggtatatttg aacttgcaaa agatggaact gtatttttag
     4921 atgaaattgc cgatttacct cttagtatgc aagcaaagtt gcttagagta ttacaggaaa
     4981 aagaaataag aagagttggt ggagatacca caataaaaat aaatcctaga ataatatcag
     5041 ctaccaataa agatttgagt aaaatggtaa aggcagaaaa atttagagaa gatttatact
     5101 atagattaaa tgttgttgaa ataaagattc ctccacttag agagagaaaa gaagacatag
     5161 gattattggt tcatagcttt ttagaggaga tatgtaaaca aaataataag cctgttctta
     5221 caattagcaa agatgtaata gatatatttc agaattatag atggaaaggt aatattagag
     5281 aattaaaaaa tacgattgaa aatattgttg tattgtcaca aaattctaaa atagatgtag
     5341 atgatgtacc aagttatatg atggattcta caaataatag tactgaagaa gaagagtatc
     5401 ccttagattt aacgaaggca actcaaaaaa ttgaaataaa gaatataacc aaggcactta
     5461 aaatgtctaa tggaaataaa gctaaagcag ctaagatatt aaatataccc agaacaactt
     5521 tgtattataa gatagaccaa tacaaaatag atgtgtctaa aatatgacat atgactaaat
     5581 tttgacaaaa tgataaactt taatttaaaa cctagtaaag ccaacacttg tataagtgtt
     5641 gtgtatatag atgtgttaaa aaaaatgaaa aagtgttgat taattgacac tttttttgtt
     5701 tgagaaaatg tttacaaata caaaaaataa catattttaa ataaatattt atataatatt
     5761 tggctaaaaa tagcgataga tgaagaatct attatagaaa aaatcttttt gttaataagt
     5821 tggcatagga attgcttata atataaagtg actttgagtg agaaaaaaat aatattaatc
     5881 ataacaaatt atgtaggttt taatttactt aatttctcag ttaaggggtt acctgttgag
     5941 gaattgagat ttaaaataat aaataagaaa ataatgtaag gaggtatttt atactatgtc
     6001 aataacttta gaaacagctc aagcccatgc aaatgaccca gcagtttgtt gttgtagatt
     6061 tgaagcggga acaattatag cgccagaaaa cttagaagat ccagcaatat ttgcagactt
     6121 agaggattct ggattattaa caataccaga aaatggatta actataggtc aagtactagg
     6181 agctaagtta aaagaaactt tagatgcact ttctccaatg actacagata acgtagaagg
     6241 atacaaagca ggagaggcta aagaagaagt agtagaagaa acagtagaag aagcagctcc
     6301 agtatcagaa gcagcagtag ttccagtaag cacaggagtt gcaggtgaaa cagttaaaat
     6361 acacataggt gaaggtaaga acataagctt agagatacct ttatcagtag ctggtcaagc
     6421 aggagttgct gctccagtag caaacgttgc tgctccagtg gcaagtgcag cagcagaagt
     6481 agctccaaaa gttgaagaaa agaaactttt aagaagctta actaaaaaac actttaaaat
     6541 agataaagtt gaatttgctg atgaaactaa aatagaagga actactttat acatcagaaa
     6601 cgcagaagaa atatgtaaag aagctaatga aactcaagag ttagttgtag atatgaagtt
     6661 agaaataata actcctgata aatatgaaac ttacagtgaa gctgtattag atatacaacc
     6721 aatcgctact aaagaagaag gcgaattagg ttcaggtata actagagtta tagatggagc
     6781 tgtaatggta ttaactggta cagatgaaga tggagttcaa ataggtgaat ttggttcttc
     6841 agaaggtgag ttaaatacta ctataatgtg gggtagacca ggtgctgctg acaaaggtga
     6901 aatattcatc aaaggtcaag taacaataaa agcaggaact aacatggaaa gaccaggacc
     6961 tttagctgct caccgtgcat ttgactatgt aactcaagaa ataagagaag cattaaagaa
     7021 agttgacaac tctttagtag ttgatgaaga agtaatagag caatacagaa gagaaggtaa
     7081 aaagaaagtt gttgttataa aagaaataat gggacaaggt gcaatgcatg ataacctaat
     7141 attaccagtt gagccagttg gtacattagg agctcaacca aacgttgact taggaaacat
     7201 gccagttgta ttatctccac ttgaagtatt agatggtggt atccatgcat taacttgtat
     7261 aggacctgca tcaaaagaaa tgtcaagaca ttactggaga gagccattag taataagagc
     7321 tatggaagac gaagaaatag atttagtagg tgttgtattt gttggttctc cacaagtaaa
     7381 tgctgagaaa ttctatgtat ctaagagatt aggtatgtta gttgaagcta tggaagttga
     7441 tggagctgta gtaactactg aaggtttcgg aaacaaccat atagatttcg catctcacat
     7501 agagcaaata ggtatgagag gtataccagt agttggtgta agtttctcag ctgttcaagg
     7561 tgctctagtt gttggtaata aatacatgac tcacatggta gacaacaata agtctaagca
     7621 aggtatagag aatgaaatat tatctaacaa cactttagct ccagaagatg ctgttagaat
     7681 aatggctatg cttaaaaatg ctatagaagg tgtagaagtt aaagctcctg aaagaaaatg
     7741 gaatccaaat gttaaattaa ataacataga agctatagaa aaagttacag gagaaaaaat
     7801 agtattagaa gagaatgagc aatctctacc aatgagtaag aagagaagag aaatatacga
     7861 aaaagacgaa aactaaaatt tagatactaa ctaagattta gacaataaca taaatttaga
     7921 tccaagttta aaacagtata gagaggtaga attataatgg ataggttaaa atatatctca
     7981 tcagagactt tttatgaagg tattatagtt gacatcaaag gtggtggagt gactatagac
     8041 ctaaaaggta gacttggaca atttaaaata cctaatagaa tgcttataac tgactacgaa
     8101 cttaagatag gtcaagaagt tggattcatg ctaagctatc ctgaggtatt aagtccagag
     8161 cctaatcaag agtatgtaga aaatattagg agagaaaaag aaaaacaagc agaaatgcaa
     8221 tcaaaacaag aagaaaaata ataaataatc gtttgaaaat agaagggaga ggaaatatat
     8281 gagccttaca acagtacaag gacttcaatc tgaaatattc gttccaataa cacctcctcc
     8341 agtttggact cctgtaacta aagaattaaa agatatgact atagctttag ctacagcagc
     8401 tggtgttcat ttaaaatctg acaagagatt taatctagca ggagatacta catttagagc
     8461 tatacctaat acagctacag ttgatgaaat gatggtatca catggtggat atgacaatgg
     8521 agatgtaaac aaagatataa actgtatgtt ccctatagat agattacatg aattagctgc
     8581 agaaggattt atcaaaggag tagctcctat gcactatgca ttcatgggtg gtggaggaaa
     8641 ccaacatgtc ttcactgaag aaactggtcc tgctatcgct gctaaactta aggaagaggg
     8701 agtagacggt gtagttatga cagctggctg aggtacttgc catagaactg ccgtgatcgt
     8761 gcagagagca atagaggaag ctggaatacc tacaataata atagcagctc ttcctccagt
     8821 agttagacaa aacggaactc ctagagcagt tgctccatta gttccaatgg gtgctaatgc
     8881 tggtgaacca cataatatag aaatgcaaac acatatatta agagatacat tagagcaatt
     8941 agttgcaata ccatctgctg gtaagatagt tccattacca tacgaatata aagctcacgt
     9001 ttaaaatgta atttatattt gaaaatatag aacccttctc ctacacggtt aggagagggg
     9061 ttttaagaac aaaggtggat attatggaag aaaaaataat gagaagactt gttataaaac
     9121 cgtttcatat gaatgaagtt aatttcggtt caaaaacttc tataaagaaa gatgtactta
     9181 caatagattt atcaagtata gatgaaataa aagaaagaga agagttaata acagacataa
     9241 aagtagacat tataaaacct ggagattatg atagagaaat taatactatt atggacatta
     9301 taccaatatc aacaaaagtt ttaggcagac tgggagaggg tatcacacat actttaacgg
     9361 gtgtatatgt aatgttaact ggtgcagatg aagatggaaa tcaaatgcat gaatttggtt
     9421 cttcagaagg aattttgaaa gaccaaatgg tatttgggag atatggaact ccatcagttg
     9481 aagattatat aatccatgta gatgtaactt taaagggagg tttacctttt gaaagaacac
     9541 ttccacttgc agcattcaga gcatgtgatg attttataca agaaataaga gcgtctttga
     9601 aaatggaaga cggaagaaat gctactcaag ttcgtgaata ttttgacaaa attcgaccaa
     9661 atgcaaaaaa ggttgtcata gtaaaacaaa tagcaggtca aggtgctatg tatgacaatc
     9721 agttattctc aaaggaacct agtggatttg aaggaggaac atcaattata gatatgggaa
     9781 atgtacctat gattatatct cctaatgaat acagagatgg tgcccttaga gctatgactt
     9841 aagagaggtg gatattatgg gaataggacc ttcaacaaaa gaaacatctc ttcatcactt
     9901 tagagaccca cttttagaca tagttgaaag tgataaggat gtagatttac ttggagtaat
     9961 agtagtagga actccagatg gaaatgaaaa taaaaccttt gtgggacaaa gaacggccgc
    10021 ttggctggaa gccatgagag tagatggagc tatagtttca tcagatggtt ggggaaactc
    10081 acatgttgac tacgctaaca catttgagga aataggaaaa agagatatac ctgtagttgg
    10141 agttacattt aatgggactc aagctaagtt tgttgtaagc aatcaatata tggataccat
    10201 agttgatatg aacaaatcaa aggaaggtat tgagacagaa gtagttggag aaaacaacac
    10261 aaatgaaata gatgctaaaa aagctttagc atttttaaaa cttaaaatga gaaaacatgg
    10321 ataaaaaatt taagtaaggg aatgagtttt gtgggaattt ctacatcaat gaaaaacaca
    10381 tcacttcata tatttagaga cccgttacta gatttgatag ataatgattc tgacatagac
    10441 ttgttaggag tggttgtagt tgggacatca gagcacaatg aatggaaaac atttttagca
    10501 actagacttg gaagatggat tgaatcattg cgacctgatg gagtgattat aactttagat
    10561 atggcaggaa accagcacat tgattttaca aatgctatag cagagtttgt aaaatcagat
    10621 atacctgcag taggtcttac tattatggga gcagatggat tagtaattac caatccatat
    10681 ttggataaag ctaccataat tgattataaa aagacaacag gctacataga aacagaagta
    10741 gttggagaca atcacatgga tgaagttgat gttaaaaaag cattggcttt cctaaaatta
    10801 aaaatgcgta aagacgcaaa ataaacctaa gggggaattt gatatgaaat ttagcagaag
    10861 tatacaagct atagactctc atacagcagg tgaagcaact agaatagttg taggtggaat
    10921 acctaacata aaaggaaatt ctatgcctga gaaaaaagaa tatttagagg aaaatctaga
    10981 ttatttaaga actgctataa tgttagagcc aagaggacat aatgatatgt ttggttctgt
    11041 aatgactcaa ccttgttgcc cagatgctga ctttggaata atcttcatgg atggtggtgg
    11101 ataccttaac atgtgtggcc atggtacaat aggagctatg acagcagcta tagaaacagg
    11161 tgtagttcca gcagtagagc ctgtaactca tgtagttatg gaagctccag caggaataat
    11221 aagaggagac gttacagttg tagatggaaa agctaaagaa gtatcattct taaacgtacc
    11281 agctttctta tataaagaag gtgttgaagt tgacttacca ggtgttggga ctgttaaatt
    11341 tgacatatca tttggtggaa gcttctttgc tataatacat gcaagtcaat taggtttaaa
    11401 aatcgaacct caaaatgctg gtaaattaac tgaactagct atgaaactta gagatataat
    11461 aaatgagaaa atagaaatac aacatccaac tttagctcat ataaaaactg tagacttagt
    11521 tgaaatatat gatgagccaa ctcatccaga agctacttac aaaaatgtag ttatatttgg
    11581 tcaaggtcaa gttgacagat ctccatgtgg aactggaaca agtgctaaat tagctacttt
    11641 acatgctaaa ggtgaattaa aagtaggaga aaaattcgta tatgaaagta tattaggaac
    11701 tttattcaaa ggtgaaatag ttgaagaaac taaagttgca gatttcaatg ctgtagtacc
    11761 taaaataact ggttctgctt atataactgg atttaatcat tttgtaatag atgaagaaga
    11821 cccacttaaa catggattta ttcttaaata agtaataaat aaattaggaa attaaaatag
    11881 aatagtagcc acttgtcggt tgctattcta tagcttttag gctataagat tcagaatata
    11941 tgaaaaaata aaaaaatagg aggatggatt atggtagcaa tcttaaaggc attatatgta
    12001 gtattcgcag caatctttgc agttgtgttt ggtaaagacg tagctaaagc aaacaaagag
    12061 gggaaactag aagaacaaag tactccaaaa gttgctgtaa caggatttat aacagacttc
    12121 tttgatacat taggaatagg gtcatttgct ccaacaatag caatgtcaaa ggctttaaag
    12181 ttaaatatac cagataaaaa aatgcctggt acattaaacg tagcacatac tattccagtt
    12241 gttacagaag cattcatatt tacatcaatc ataccagttg atggtgttac tttagtatct
    12301 atggttgtgg cagcagcagt tggttcatat ataggtgcag gaataatagc taagatggac
    12361 gagagaaaaa tacaacttgt tatgggtgta actttagcat taacagcaat attaatgtta
    12421 ttaggtcatc cttggattaa cgtattacca ggtggaggaa atgaattagg tcttactggt
    12481 gttaaattgg taataggtgt aataggtaac tttatactag gagctttaat gacagctggt
    12541 ataggtctat atgctccagg tatggcaatg gtttatttct taggaatgtc tgcaaaagtt
    12601 gcattcccaa tagttatggg ttcttgtgca ttattaatgc cagttgcaag tatgaaattt
    12661 ataaaagaag atgcatatac taaaaaagca tcagttataa tagctatatg tggtttaata
    12721 ggagttttcg tagctgcata tatagtaaaa actctaccaa tggatatatt aaaagcatta
    12781 gtaatagtag ttatagctta tacttctgct acaatgataa tggctgcaaa taaaaacaaa
    12841 aagactgctt agttaaagac tacttagtgt ttattaaata ttacagctaa taataaagat
    12901 tataaactgc tttaaaatat tgaattaata ttttagggca gttttttatt tgctaaatta
    12961 atataaagca attaacatgc caattatgta atatatatag tgatttagta gatttaatgt
    13021 aaaaaacgtt gtattttcaa agaataaagc ataatttaaa gctaaatatt taaattttat
    13081 aatcaataag ttcaataaat gttcatatat taatacagat ta
//
LOCUS       DACTGW010000097        13109 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.097, whole genome shotgun sequence.
ACCESSION   DACTGW010000097 DACTGW010000000
VERSION     DACTGW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.097"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            109..1167
                     /locus_tag="I9573_002129"
     CDS             109..1167
                     /locus_tag="I9573_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAT4796748.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVGYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            1309..2133
                     /gene="spo0A"
                     /locus_tag="I9573_002130"
     CDS             1309..2133
                     /gene="spo0A"
                     /locus_tag="I9573_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HAT4796749.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            2546..3667
                     /locus_tag="I9573_002131"
     CDS             2546..3667
                     /locus_tag="I9573_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796750.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            3685..4560
                     /locus_tag="I9573_002132"
     CDS             3685..4560
                     /locus_tag="I9573_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HAT4796751.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNSLGNTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            4584..5276
                     /locus_tag="I9573_002133"
     CDS             4584..5276
                     /locus_tag="I9573_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT4796752.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            5284..6108
                     /locus_tag="I9573_002134"
     CDS             5284..6108
                     /locus_tag="I9573_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAT4796753.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            6113..7180
                     /locus_tag="I9573_002135"
     CDS             6113..7180
                     /locus_tag="I9573_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HAT4796754.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGKIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            7182..7718
                     /locus_tag="I9573_002136"
     CDS             7182..7718
                     /locus_tag="I9573_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAT4796755.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            7786..8391
                     /locus_tag="I9573_002137"
     CDS             7786..8391
                     /locus_tag="I9573_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796756.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTVSIGIIKFLAK"
     gene            8403..9317
                     /locus_tag="I9573_002138"
     CDS             8403..9317
                     /locus_tag="I9573_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAT4796757.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            9359..10531
                     /locus_tag="I9573_002139"
     CDS             9359..10531
                     /locus_tag="I9573_002139"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAT4796758.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            10578..11390
                     /locus_tag="I9573_002140"
     CDS             10578..11390
                     /locus_tag="I9573_002140"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAT4796759.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            11594..12919
                     /locus_tag="I9573_002141"
     CDS             11594..12919
                     /locus_tag="I9573_002141"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAT4796760.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEVDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSIKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
BASE COUNT         5326 a         1265 c         2455 g         4063 t
ORIGIN      
        1 tattaaaaat cacttattaa gattttaaat aaactttttg aatgaataaa ttctcaggaa
       61 aaaggaatta taaatacata ataatacagc ataggagtag atggatatat gaacaaaata
      121 aataaaaatt tcaaaaatat ttggataata ttaggagctg tatcgatttt taccatggtt
      181 ataattttta ataaaaattg taatgttaat aaagagactc aaatagaagc ttttacaatt
      241 aagaatcatg ataaaaaatt tgtatatcct atgggaaata taattggagt aaaagcaaat
      301 acagatggtg tattggtctt aggatatgag gaagaggatg taggctatat tggaggcata
      361 caaataggtg ataatatagt aaaaatagat aataaaagga taaaaaatag tcaagatgta
      421 tcagagattt tgaataagat taaaaaaagt aaagttgaag tgacttttga gagaaaaaat
      481 aagtataaaa cagaagtaat agagactaaa aaagaaaatg gcaagtataa acttggatta
      541 tgggtaagag ataaaatatc tggaattgga actatgactt tttacgaccc tagtatggaa
      601 aagttcaaag caatcggtca tgccataaaa gattcggata ctaatgaact attaaagata
      661 aagcaaggct atatatataa gccagaacaa ttaaaaatag taaaagctag taatgaaaaa
      721 gtaggtaaaa taaaaggaga ttttaatgac agcaatttaa tgggtaattt ctcaaataat
      781 tcagagctag gtataagtgg taatattaca gaaaaccata ataaagagtt taatgtagca
      841 aataaagaaa agcaattaat agaagttgga agaccgcaag atgtaaaaat aggagatgca
      901 gtaattcttt ttgaggataa aaacaaaaat ataacaagct atgatataaa aatagaaagt
      961 atagtatatg ataaaggaaa ttatagagat atggtaataa aagtagtaga tgacaagtta
     1021 ttggaataca caggaggtat cgtacagggg atgagtggag ctccaataat acaaaataat
     1081 aaaattattg gtgcaataac tcatgttttt agagataatc cgaaaaaagg atatggtatt
     1141 tttatagatg aaatgataaa attgtaggtg aggcattaaa aaattttatt attttatcaa
     1201 ttatctagga ggaatataat tttggagtgt cgaatatgct ttagagtaga taattaggaa
     1261 gcaattgtgt aaaaagttta gttttctgta ataagaagat gttttttaat ggggggattt
     1321 ttagtggaaa aaatcaaaat agttttagca gatgacaata aggatttttg tcaggtatta
     1381 aaagagtatt tgtctaatga agatgatatc gatatattag gcatagctaa ggatggaatt
     1441 gaagcattag acttagtaaa aaagacacaa ccggacttat taatactaga tgtaataatg
     1501 ccacatctag atggattagg tgtaattgaa aaattgaata ctatggatat acctaaaatg
     1561 ccaaaaataa tagtactatc agcagtaggt caagataaga taactcaaag cgcaataaat
     1621 ctaggagcag attactacat agtaaagcca tttgattttg ttgtgtttat aaacagaatt
     1681 agagagctag tttctaatag agttacacaa gttgagccaa aacctagacc agttcaagag
     1741 actcaaatga ctagaagtga ttttgttaag aatgtaggaa atatagagac agaaatcaca
     1801 aatataatac atgaaatagg agtaccagct catataaaag gatatttata tttaagagaa
     1861 gcaataaaaa tggttattga taatgttgag cttttaggtg cagtaacaaa agagttatat
     1921 ccaagtatag ctaaaaaatt caatacaaca ccaagtagag ttgaaagagc aataagacat
     1981 gcaatagagg ttgcatggag tagaggaaaa gttgacacaa taaatcaatt atttggatat
     2041 acggtacaca atactaaagg aaaaccaact aattcagaat ttatagcaat gattgctgat
     2101 aaattaagac tagaacatag tatggttaaa taaacaagac ataaaaagta aggcttttta
     2161 attaaggcat tggctataaa tgcgtattac aagcagcgaa acggtataac cactagggtt
     2221 ataccgtttc gctattttaa taaatataaa aattttcttt attatttgct tactatatca
     2281 atataataat tttattatac tatggatata gtatgtgtct ttacaagttg taaactgaca
     2341 gtggtttatt ttttaatata aatattgact ttgatgcagg taaactttgt atttttaagc
     2401 gtattatgga atatgttaaa taaaaaaatg atgaaatata gtattgtaaa tgccaaagat
     2461 gcaaaacaaa cttaaaacat ttattttatt gttaagtaat gctataatat aatgtgattt
     2521 taataatgat agtggaggtt taaatatgag agtcgaggcc cctataaaag tagatcgaaa
     2581 aaccaaaaaa cttgctaaaa gagttgaaag tggggaaata gcagttataa atcatataga
     2641 catagatgaa gttgctgcaa actctttagt agaagctaaa ataaaacttg tcataaatgc
     2701 ggctccttct ataagtggta ggtatcccaa taaaggtcca gggatattaa ctgaaaataa
     2761 tatactaata attgataatg ttggagaaga gttatttgaa gaattaaaag aaggagaaac
     2821 tatagaagtt gtagatggaa agatatatag aaaaggaaaa tttttaggtg caggagaagt
     2881 tttagacaag tatgaggttt cacaccaaat aaaggctgcc tatgagaatt tagctgtaga
     2941 attggataga tttatagata atactataga ttatgctaaa aaagaaaaag gatttatact
     3001 tggagaagta gaaattccaa aagtgaaaac taattatgct aataaacatg tattgatagt
     3061 tgtaagaggt caggactaca aagaagattt aagtactatg ctttcttata tagaagaggt
     3121 aaagccagta ttggttggtg ttgatggagg agcagacgct ttaattgagt ttggatatac
     3181 tccagatgtg attgtaggtg atatggatag tgtaagtgat gaagctttaa aaaaggctag
     3241 tgaaatagta gtacatgctt atacagatgg aagagcacca ggtcttaaga gggttgagga
     3301 attaggctta gatgcagttg tatttcctgc gccaggaact agtgaagata tagctatgct
     3361 tatcgcctat gagtataagg ctgagttaat agtagcagtt ggtacacatt caaatatgat
     3421 tgattttctt gaaaaaggaa gaaaaggtat ggcaagtact tttttagtta gactaaaaat
     3481 aggttcaaag cttatagatg ctaaaggcgt taatttgtta tacagaagta aattaaaaat
     3541 aaagtacata tgggcattaa tagcaacagc attatttcct gtgttggtag ttgcatcatt
     3601 gtcaccagga gttcaacagt tcatacaact aatgcaatta aagtttagag tattattaca
     3661 gatgtaaatt ttaggaggat aaatatgcat attaatatga aatattatat agtcactata
     3721 ggggctatat ttatagcttt gggtataggc atgttagtag gatttaattt aaataataat
     3781 caacaattaa gtgaacaaca agcaaacata ataaatgatt tggatgataa gtttaatatc
     3841 ttaaaagaaa aaaatgataa acttgacagt gacttagcaa gtgtaaatag agattatgaa
     3901 gaggcagtta attttataaa taaaaatgta gataagattt tagctggttc gctgaatggt
     3961 aaaagtatag gaataatatc ggctaatgaa aatgacgatt atacaaaaaa tatagaagac
     4021 ataataaata aatctaatgg aagtattgca tttaatatag tattaaaaga aaatataaca
     4081 aatccagaaa agttaaagga aatgtcaact aaactaggta cagatgtaaa aaatgcaaat
     4141 gatgcagtta attatataat tgacaccctt aaaaaggaag atgcaagtga cattttaaca
     4201 tatcttcaag aactagatgt aataaagttt aactctcttg gaaatacata tctaaaatat
     4261 gattctgttg taatagctgg tggaaatgat gcaaaagaca gtacaaaaca gtttgaaaaa
     4321 atagaaaaat ttgtagtttc aaaacttaaa tcagagaata aatatttagt agaagttcaa
     4381 aatacaggag ttaaaacttc ttatgtagaa ctatattcta aaaacaaagt tgcaactata
     4441 gacaatattg atgaaggtat aggaagtata tctctagcta ttcttctaca acaaggtaat
     4501 atagttggta attttggtag attggatact gcaacttcat tgttgccatc tattaaatag
     4561 aaataatttt aaggagagaa aaaatggatt cttatataag tataataata ccagcttata
     4621 atgaagaaag tagaataaaa tatacattag aaagcatatt ggacattaag caaataaatg
     4681 aaataatagt tgtggatgat ggttcaattg acaatactgc taaggttgta tctgagctag
     4741 aaaatgaaaa gattaaattt tttaagttag ataaaaatag aggtaaagga tatgctttaa
     4801 attatggtat caaattagca atgaaaaatg cagatattat aggttttctg gatggagatt
     4861 taggaagctc tgctaaagaa gttgaaaaat taatagtacc tatattaaat ggtgaggcgg
     4921 atgttacaat agccaagttt cctcctgcaa agagaaaagg aggtttggga tttgtaaaag
     4981 gtttagctaa aaattctgtg tttgagatga ctggtgttga gttagatgca actttatcag
     5041 gtcaaagaat atttaaaaga gaagttttag aaaagtttga tgaaatacct tttggatatg
     5101 gagtagaagt tggaatgacc attgatatat taaagtatgg atttaaaatt aaagaagtat
     5161 tagtaaatat gactcataat gaaacaggaa gaaatttaaa aggttttatt catagaggga
     5221 aacagtatta tcacataaaa aaggttttag gacaaaaaaa gaaagaatgg aggtaatatc
     5281 ttagtgaaag attatatact ttataccatt ttattattaa ctggattatt tggaacctat
     5341 gctgtaattc cactatttaa aaatttattg ataaatggaa atgtacttag accaaactat
     5401 aaaaaagata tgataccagt aagtatggga attgtatttt tgcctatgat tgtaatcaat
     5461 ggaataatac ttgctttctt tactacagaa tttaaggatt tagcgtatat atttatgttt
     5521 atgtttggta tgatatccat gttttttgca gggatactag atgatgtcat tggaaataga
     5581 gatgtaagtg gtttaaaggg gcattttaaa agtttattta aaggaagtct tacaacagga
     5641 ggttttaaag ctttatttgg tggatttgta gggcttatag tttctgttgc tatatcaaaa
     5701 gacataattg atataatagt aaatacacta ataatagctc tatcaactaa tttaatgaat
     5761 cttttggatt taagaccagg aagggcaata aaagcatatt tattcataat ggtaattata
     5821 tttttaacat tgacaggatt tgttcaagtt cttccattac ttatagtacc aaatgtactt
     5881 gcatacttta attatgattt aaaagcaagg gctatgatgg gagatactgg ttcaaacgtc
     5941 cttggaatat ctattggaat gctcatttct tttgggtatc catttaacat tagattagga
     6001 tggttaatat ttgtagttat tatgcatata ttcactgaga aatactcatt aactaagata
     6061 atagaaaaaa ataaactatt aaattttata gataaattgg gaaggtagta ttatgataag
     6121 caaaagagtg gggattgttg aatcaatagt aagtcaaact gaaactctag atgatataag
     6181 agtaaatata aatggaaaaa tacaaagggc atataattat cccaaaatat ctggaactat
     6241 aaatataggt gatgaagtgg ttttaaatac aacagcagta gaactgagct taggtacagg
     6301 tggataccat tttgtaataa ctaatttaaa taatatagaa tctactttaa ctgaaggtgg
     6361 gcatataatg aaacttagat atacacctct tcaaataaaa gtggattctg ttgaggaaca
     6421 agaaagtatt tatcatgata aatttgcgaa ttttaaagga ttagaaggtc tttctgttgt
     6481 agtagggact cttcatagta tgttaactcc atttgcagct tcatttaaaa gaaataatcc
     6541 aaataaaaaa ttagtatata taatgactga tggagcatct cttccaattt atttaagtaa
     6601 aaatgtggac actttaaaag aaaaaaaatt aatagatagt acgataacta taggaaatgc
     6661 gtttggtgga gattatgaat gtataaatat atatactgca ttaattacag caaaagaaat
     6721 actaaaagcg gatgcagtat ttgtcagtat gggacctgga attgctggga caggaacaaa
     6781 atatggattt acagggattg aacaaggctc tattcttgat gccgtaaaaa aacttgaagg
     6841 aagagcaatt gcaattccaa gaattagttt tgctgacaaa cgagaaagac atcaaggtat
     6901 ttcacatcat agtatgactg tatttaaaga aattgtaaat gtaaatgtag acataccaat
     6961 aactatatat gatgatgaaa aattgaactt tataaaagag caaatcaatt taaatggttt
     7021 agacaaaaag cataatataa tttatataga taataataaa acgaaagagg atttagacta
     7081 ttttaatcta aaagtaaaga gtatgggaag aaattatgaa caagatgagg catttttcaa
     7141 agctgcaagc acagcagcat attacttaat ggaggtttaa gatggtatta gaagaaaaaa
     7201 ctattagtag tgatagggtt tatacaggta aagtaattac tttaaaggtt gatactgttg
     7261 aaattccagg gcaaggatat caaaaaaggg agttagtaga agttggtgga gcagtaggaa
     7321 tagttgcaat cacagatgat aataaagttg tactagttaa gcaatttaga aaacctatag
     7381 aaaagccaat ctttgaaatt ccagctggta aactagaaaa aaatgaaagt ccaaaagaat
     7441 gtgctgaaag agaattaaaa gaagaaactg ggtatagtgc aaaaaatatt aagctaatac
     7501 ataaattttt cacttcagca ggtttttcta atgagataat gtttgtatat ttagcaacag
     7561 gtcttactcc aggagagaat aatttagatg cagatgagtt tttagatgtc tatgaaattg
     7621 agcttgaaga agcatataac atggtattaa aaaatgacgt tgaagatgca aaaacatcta
     7681 ttggattatt attagtaaaa gatatgttta aaaattaata tttataaaat gaataaccct
     7741 ttgtcctaaa gcatattatt ttataaagga caagggggaa ttaaattgag aagaacatat
     7801 agaaaaaatg attttagaga attaaataga caaataatta ttataggatt gttgtttatg
     7861 atgtctattg ttgttgggtc ttacataaat aaaatattgc caggttctag taacaatata
     7921 ttaaacaata taaatcctgc tgtagagtat tataatctaa atataagcat aaaagataca
     7981 gtgatacaaa atttaaaatc agatgcaatc tttatgggct caatagctct attaagtcta
     8041 tttgtagtaa caattccagc agtgttggta gcttttgtat taaaagggat gtcaattggt
     8101 tatacaataa atagttgtat attggcatta aaatttaaaa gtataaaaat gattttaata
     8161 atattattta aaaatttgat tattattcca tgtgcaatca tattagcatt aattagttta
     8221 agttatttta aagaaatggt ttatgaattt aaaaagaaaa atagaaaaaa tatgcaattt
     8281 ttaattaaga ggtatatact aaatattata attataatag cactatcatt ggggttgcaa
     8341 ttaatattaa atactgtaag tattggtatt attaagttcc tagctaaata aaggagttta
     8401 taatggatat tatagaggga tatatagatt acataaaaga taaaaaaaaa ttatcagaga
     8461 acacagtggc atcatacttt atggatataa aaaaatatat ggaatatata aatcaaaaaa
     8521 gcattaaact agtagatata gtagaaaatg atattataag ttacttaata aagttggaaa
     8581 aagataatgt atcaatagct acaatagcga ggatgatatc ctctataaaa tcttttcatg
     8641 attatttatt tttaaatcat atatgtacaa ataatcctgc aaaggatata aaaaaaccta
     8701 aaataaaaaa agaaaatata aatatactta cagaagaaga aattgaaaga ctattaaatt
     8761 ttccaaagtt aacaacacct aaattgatta gggacaaggc tatttttgaa gtattatatg
     8821 gtactggtat aaaagtatct gaattagtag agatgaatat agaggatatt gacctagata
     8881 ttgattatat atattgtaat tcatgttgta aaaatcaaag agtgatacca ctttgtgata
     8941 ttacaaaact ttacttagaa aagtatctaa aagaagcaag acctaaaatg gctgtagaag
     9001 gtgaaaaatc attattcgta agttcattag gtcaaaggtt tacacgacaa ggtctttgga
     9061 aagtcataaa aaaatattct aatctagcca atatagataa aaatataaat cctacaatgc
     9121 ttagacattc atttgctatt catttgttaa atgaaggtgc taacattgct gttgtaagta
     9181 aaattttagg aaatgttaat ttatctagtt tgcaagtata tttaaatcat atagataaaa
     9241 acgtgagaag agaaataaag gaaaaacatc ctagaaatga tgttgatgta gaattacaaa
     9301 aagcagaagc taaatagact atatagcaaa ttttataatt taaaaggagt atatttatat
     9361 gagcagagta atttggattg taattgatag tgttgggata ggagcattac ctgatgctga
     9421 aaaatttggt gatagtaagg atgtaagtac ccttggaaat atatttaagg aatatccaga
     9481 tattcagatt cctaatatga gaaatttagg tataggaaac atagatggta tagatttttt
     9541 tgaaagtata aaagaaccaa taggttgttt tggaaagtgt aaggaaatgt ctcagggaaa
     9601 agatactact acaggtcatt gggaaatgac tggtataata gttgacaaac catttaagac
     9661 atttgaacat ggattctcta aggagataat agaagagttt gagaaaaaaa ctggaagaaa
     9721 agtagtagga aataaaccag cttcaggaac tgtaataata gatgagtatg gagagcatca
     9781 aataaaaact ggagatgtaa tagtatatac atcagctgat agtgtatttc agattgctgc
     9841 taatgaagaa gtaattcccc tagaagaact ctataatatg tgcaaaatcg ctagggaaat
     9901 aatgatgggg gataatgcag tagctagagt aattgctaga ccattcatag gtaagaaaaa
     9961 aggtgagttt gtgaggactt caaatagaag agattattca ctagacccat ttgaaccaac
    10021 agttcttgat aatataaaag aatcaggact tgatgtttta gcagttggaa aaatagaaga
    10081 tatttttaat ggaaaaggaa ttacagatgc tatccacact aaaagcaata tggatggagt
    10141 ggatgaaact ttaaattata tgaaacaaga taataaaggt ctaatatatt caaatctcgt
    10201 tgattttgat tctaaatatg gtcatagaag agacccagaa ggatataaga aagctcttga
    10261 agagtttgat agtagacttc ctgaaataat ggctaatatg agagaagatg atattttaat
    10321 aatcaatgca gaccatggaa atgacccaac atataaaggt acagaccata caagagaata
    10381 tatacctgtt atgatttatg gcaataagat taaaaaaggt tttaatttag gagtaaaaga
    10441 tacttttgct gatattggag caactgttgc agatatacta aatgtaaagt tgcctaaaca
    10501 tgggtcaagc tttaaagaag atttatttta aactataaaa tgataaaaac ttagcttaat
    10561 aggatggaga gattggaatg tttgaaaaga tagcacaaag tagtaaattt ataaattcta
    10621 aaagtaatat aaaacctaag attggtttga tattaggttc tggactaggt gatttagcaa
    10681 atgatataga agaccctgtc acaattaaat atagtgaaat tccaaacttc cctgtatcaa
    10741 cagtagcagg acatgctgga caacttgtaa ttggaaaact tgaaggaaaa gaagttattg
    10801 ctatgcaagg tagatttcat tactatgaag gatattctca gaaagaagct acttttccag
    10861 taagggttat gaaagaatta ggagtcgaga ttcttatagt aactaacgct gctggaggcg
    10921 taaataaaga atttaaagca ggagatttga tgcttataag agaccatata aactttagtg
    10981 gaagtaatcc attagtagga aaaaatgatg acagatttgg tgctagattt ccagatatgt
    11041 cagatgcata ttcttctaaa tatgtagatg tagttaaaaa ctgcgctaaa gaatgtaata
    11101 tagatgtcaa agaaggcgtt tatatgtttt ttagtggtcc aaactatgaa acaccagcag
    11161 aagtgagaat ggcacaaatt ttgggagctg atgctgtagg tatgtctact gttccagaag
    11221 tcatagtagc ttctcactcc aatataggag ttattggtat atcttgtata acaaatatgg
    11281 ctgctggcat cttaaatcaa ccactaagtc atgaggaagt aatagaaact actcaaaaag
    11341 ttaaatcaga atttatgaat ttagttaaaa caacggttaa aaatttataa gttaaagctt
    11401 attatatatt ttttgaggaa gtttataata aaatagttaa tgtttaattt gaaaagaatt
    11461 aataaaaagc tgtcttgata tagaaagtta tttctaataa aggacagttt ttttataata
    11521 ctacaacatt tgaaagtgtt ttaaagttta atatttattt aaattattat agattagaat
    11581 ggagatttca attatgagaa tgtatgatat tataaaaaaa aagagagata acttagagct
    11641 tagtaaaaaa gagataaact actttataga aaactattca aagggagata tacctgacta
    11701 tcaagcttct gcactattaa tgtcgattta tttaaataag atgaataaac aggaaactgt
    11761 acatcttaca gaagcaatga tgaactctgg agacatgata aacctgtctg aggtagatgg
    11821 aataaaggta gataagcata gtactggtgg tgttggagat aagactacca tagctcttat
    11881 acctttggtt gcttcatgtg gagcaccagt tgcaaagatg tcaggtagag gtcttggtca
    11941 tactggtggt actattgata aattggagtc tataccaggt ttttcgacag aaatggagat
    12001 aagtaaattt attgattctg taaataagtc taaaatagca gtttgtggtc aaagtgcaaa
    12061 ggtagcaatt gcagataaga aaatatatgc tcttagagat gttacagcaa ctgttgataa
    12121 tatatcattg atatcatcaa gtgtaatgtc taaaaagtta gcttcaggag ctgatgcaat
    12181 tgtgttagat gtaaaaactg gtgatggagc atttatgaag acattagaag agtcttttga
    12241 gttagctaaa tctatggttg atataggtac tggtatgaat agagatacaa tagggattgt
    12301 aacagatatg gatgaacctt taggttttgc agtgggaaac tctttagaag ttatagaagc
    12361 aattgagaca ttaaaaggaa atggccctaa agaccttgtt gagctatgtg aagtattagg
    12421 agcatatatg ttggttttat caaaggtagc acatgatttt gaagatggtg ttgaaaagat
    12481 aagagaatca ataaaatctg gtactgctat acaaaaactc aaagagttta tagagaatca
    12541 aggtggtaat aaagatgtag tagatgatta ttctttattc aaaaaatcaa agtataaata
    12601 tccagttaaa tcaattaaat caggttatat aagtaaaatt aaagctgaag atgttggtgt
    12661 atcagctatg attcttggag caggtagaga aactaaagat gatattttag atttatcagc
    12721 agggataatt ttagaaaaga aagtaggaga ttatgtaaat gaaggtgaag ttttagctta
    12781 catgtattat aatgatgaac aaaagctttc attggcaaag ggaagattta ttgattccta
    12841 ctctattgta gattcaaaaa tagaaaaaaa taaattaata tatggtattg taactaaaga
    12901 agagattaaa aagttttaga aaattttaga aaagtgatta taaaaggcct aacatgtcaa
    12961 aaataatgtt aggtttttta ttttttgata aactataaat tataccaaaa atatatattt
    13021 taaaacattt tttacaatat aaataagttt attctacaag atgattgaaa attaaagtat
    13081 ctagttttat aatagaaaat ggataaata
//
LOCUS       DACTGW010000098        13097 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.098, whole genome shotgun sequence.
ACCESSION   DACTGW010000098 DACTGW010000000
VERSION     DACTGW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.098"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            103..1767
                     /locus_tag="I9573_002142"
     CDS             103..1767
                     /locus_tag="I9573_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAT4796761.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            1780..4230
                     /gene="recJ"
                     /locus_tag="I9573_002143"
     CDS             1780..4230
                     /gene="recJ"
                     /locus_tag="I9573_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAT4796762.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            4243..4755
                     /locus_tag="I9573_002144"
     CDS             4243..4755
                     /locus_tag="I9573_002144"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAT4796763.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            4829..7036
                     /locus_tag="I9573_002145"
     CDS             4829..7036
                     /locus_tag="I9573_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAT4796764.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            7061..7510
                     /locus_tag="I9573_002146"
     CDS             7061..7510
                     /locus_tag="I9573_002146"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAT4796765.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNASRPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            7525..8139
                     /locus_tag="I9573_002147"
     CDS             7525..8139
                     /locus_tag="I9573_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT4796766.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVSDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            8230..9786
                     /locus_tag="I9573_002148"
     CDS             8230..9786
                     /locus_tag="I9573_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HAT4796767.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNRLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDKKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEELNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            9776..11038
                     /locus_tag="I9573_002149"
     CDS             9776..11038
                     /locus_tag="I9573_002149"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAT4796768.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            11112..12899
                     /gene="aspS"
                     /locus_tag="I9573_002150"
     CDS             11112..12899
                     /gene="aspS"
                     /locus_tag="I9573_002150"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAT4796769.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTKMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDIQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         5316 a         1360 c         2463 g         3958 t
ORIGIN      
        1 aagaggatat aggatattag aatatcaatt ggtgaaataa tgtcatatac gataaattaa
       61 ggtgtaagtt tttgggtata cctatcttag gagtgaaaag atttgagaat aagttataga
      121 aatttaaagc gatatagaga aattggatat gttttaataa agtatggttt tagttttatt
      181 gtagaaagac taaatataga aggaattgcc tataaaatac cactatttga cccaccagaa
      241 gaaataaaaa atatgacaac tggtgagaga atgaaaagag ttttagaaga actaggacct
      301 acatatataa aaataggaca gatattgagt acaagaaaag acttgttaga ccaggatata
      361 attgatgaaa tatcaaaact cagagatgat gtagagaaat ttgattcaaa tatagctatt
      421 gatatattta aagaagaagt gggtttaagt atagaagaaa tattcttaga atttaaagaa
      481 gaaccaatag cagcagcttc aattggtcaa gtttatgaag gtgtacttaa aacgggagaa
      541 gaagtaatcg taaaaattca aagaccaaat atagagaaaa taataaaatc tgatttagag
      601 attcttagaa ctattgcaaa tactttaaaa gatttaaaaa aggattttaa tttagattta
      661 gtacagatga ttgaagaatt tcaaactcaa ttaatgaggg aacttgatta tacatttgaa
      721 gccataaatg caactaaatt ctctaggata tttaaaaata gtgatgaagt atatatacct
      781 aaagtatact cagaatataa tactaagaaa attttagtaa tggagaaagt taatggtaca
      841 aagttaagtg atgtagaaaa aataaggaga ctaggttata acacaaaaac tattgttgaa
      901 ataggagtta ggtcattttt tacacaggta ttatctcatg gattttttca tgcagaccca
      961 catccaggta atatatttgt agttgctaaa aataagatag catatataga ttttggaatg
     1021 attggaatta ttgataataa aactttaaac caattaaatg aaattgcatt agcaggtgta
     1081 gaaaagaatg ttgacaagat aatatatcta ttgatagaaa tggatgcatt aaatggagag
     1141 gctgatatta agggacttag acaggattta ctttatctaa tacattatta ctatgatatt
     1201 tctatagaaa aaataaatgt aacagatata ttaaatgaac tatttaggtt ttttagacag
     1261 tataaaatag tcatgcctgc acaatttgta actcttgcaa agacagttat aacattagaa
     1321 ggaacaagta gaactttaaa cacagatttt tcttttggtt ctatgggaaa agagtttatg
     1381 aaacatcatt ataagagtaa atttaaccct aaaaatgttg ttttaagttc aagacaaaat
     1441 gtagaagaaa tattactgga cataaaaact attccaaagc aattaaaagc aattttaaaa
     1501 aatatagaaa gaaataatat aaaaatgcaa attgaagatg ttaaaatgac gaggttagaa
     1561 aattgtataa tagagctaac atctcaaata tctttgagtt tagtgttggc atctattata
     1621 gtaggttctt ctcttataat tgcatctcca aatatagaaa ataatatatg gataaaattt
     1681 actgcaatag cgggtttctt tatatccttt ataataggcc tttgcttagt tataagaagt
     1741 attaggtcaa aatacaaaaa agattagggg gaattaaatt tgaaaaaatg gatattgaag
     1801 tataaaggta agatagagga aagtgaattt agcaaaaaac ttaatatttc cccggaaatc
     1861 tgccaaattt taaaaaatag agggatatgt actgaaaaag attcagaaat atttatgaat
     1921 ccatctttag attatttaag agaccctttt ttaatgaaag atatgcaaaa agctgttgat
     1981 agaataaagt tagcagttga gaagaacgaa aaaatttgga tttacggaga ttatgatgtt
     2041 gatggtgtat catctacatc catcttgtgt ttatatttta agagtatagg ttacaatgtt
     2101 gattactata ttcctaatag attagaagaa gggtatggta taaatgagga tgctattaag
     2161 cttataagct ctaggggatg tgacttgatt ataagtgttg actgtggaat tacatcagtg
     2221 tctgaggtca atacagcgaa tgatttgggc atagatgtta taattacaga ccatcatgaa
     2281 tgtcaaagtg aaattccatc agcctttgca gtaataaatc caaaacaaga agattgtaat
     2341 tatccatttg attcactatg tggatgtggt gtagcattta aaatgattca agccttgact
     2401 ccaaaagaag aatttaaaac tagtatgtat aattatttgg aaatagtaac actagcaact
     2461 atttgtgata ttgttccact tatagatgaa aatagaataa ttgtaaaaaa tggtttaaaa
     2521 tcaatgaaag aaggcaaaaa tattggatta agggagctta taaaagtttg tggtgtagaa
     2581 agtgataaga taggttcttc tcatataggt tttgcaatag gtcctaggat aaatgcttca
     2641 ggtcgattgg gatattctta cttaggagta gagctattta ctactcagtc acaagaagag
     2701 gcagtagaaa tagctagtat attggaagaa aaaaataatg aaagacagat gatagaagct
     2761 aagatgtatc atgaagctga agaaatgcta aagtctaata gcagatacaa tgatgacaaa
     2821 gtgttggttt tagccaaaga gggttggcaa catggaataa taggaattgt agcatcaaag
     2881 ttaactgaaa aatactacaa accaacaatt cttctaggta tagaaaatgg ggaggctact
     2941 ggttcagcga ggtcaataaa aggatttaat atatttgaag ctctcataaa gtgcaaagac
     3001 ttgatgacta agtttggtgg tcatgaacaa gcagcagggt tatccttgga ttcagacaat
     3061 gtagaaattt tagcaaatga aataaataaa tttgcagatt ataatttaac tgaagatgat
     3121 atgatagaga atgtaaatgt cgagtttgaa ttacaagaaa atgtaataaa tttaaatctt
     3181 gtagaagaat tacataagtt agaacccttt ggattaaata atcctaatcc tagatttata
     3241 gttagaaatt atattttaaa agatttgaaa gttataggta agaatcaaca acatctgaaa
     3301 ttaagtatag aaaaagagaa aagctatgaa tgtataggat ttaatatgag tcatttaaaa
     3361 tcaatgtata aagtaggaga caaagtagat gtactattcc aattagatga aaataactat
     3421 atgggaaata ggaaagtaca atttttactt aaagatatac gattggctcg acctaaaagt
     3481 gcaagtaatg acaaactttc attgaaatta atgtcaaaaa taataccaaa agatactcaa
     3541 agcctgtata atatatctgt gtctgatttt gaattatttg atggaaatac agatataaat
     3601 atatttgatt attttgaaaa agatacacta ataatatcta attctataaa tggattttat
     3661 agagcaatgt cagatatatc acttatagat ttggatttta acataaatta caatattatt
     3721 gaagatgtta gcaaaaatac tgataaatta gagcttatat tttctcctaa tattgataaa
     3781 atagacttaa aaagatataa taatattatt ctttatgatt atttatacaa taaaggcgaa
     3841 tattcgtata tatatgagaa taaaagagaa gaaagtgaaa ttataaaata ttataataaa
     3901 acagacctat tatatttaaa gaatgtagtt agtaatatag ttccatcaag agatgaattt
     3961 ataacaattt ataaacaagc acttataaaa aaagaaattg atttggatat ggtaaatata
     4021 agggaaactt ttaatgtaat tccattaaag ttttttacga tattaaatgt atttagagaa
     4081 ttaaatttac ttgactttaa tttaaattat gaaaaaaatt ctgtactcat aagaatattg
     4141 ccaaaaccac agaaaaaatt agacttaaat gagagtttaa tactcaataa tctaaaaaat
     4201 ttagaaaaac aatacaatag cagttattag gaggcgtttc ccatggattt aaaaaacttt
     4261 ataaggaata ttgatgattt cccaaaacca ggcatagatt ttaaagatgt gacaactcta
     4321 tttaaagatg gagatgcatt taaatatgca gtggattcta tagttgaaga attgaaagat
     4381 aaagatgtag atttagtaat agggccagaa gcaagaggat ttttgatggg tacacctgta
     4441 gcatatgctt taggagtagg atttgttcca attagaaaac ctggaaagtt accaggtgaa
     4501 gttgagagtt atgagtatgg attagagtat ggtacagata ctttagagat acataaggat
     4561 gcaattaaaa agggtcaaaa agtagcaata gtagatgatt tattggctac tggtgggact
     4621 atggaagccg ctgccaagct tgtagaaaaa ttaggtggag aagtagtttc tatgcaattt
     4681 ttgattgagc taaaattctt aaatgggaga gaaaagttgt ctaactatga tgttaattct
     4741 cttataaaat actagtactt ttataatagt tggtataaca ccaactattt tcatatatat
     4801 taaaatttta tacaaaatag gtggttatat gcatgataaa gaattacaag aaataattga
     4861 aaaaataaaa gtttatgcac ctaatgcaaa ttttgaaatg gttgaaaaag cctataatct
     4921 tgcaaaaaaa gcgcatgaag gacaatatag aaaatctggg gaaccatact ttattcatcc
     4981 attagcagtt gcaaatattt taatagagat ggaattagat attgaaacta taacagcagg
     5041 tttacttcat gatgttgtag aagatacaga atatacctat gaagacataa aaaaagaatt
     5101 tggcaaagaa gtagcggatt tggttgatgg agtaactaaa cttggtcaaa taaaatatcg
     5161 ttcgaaagaa gaaactcaat ctgaaaattt aagaaaaatg tttttggcaa tggcgaagga
     5221 tataagggtt atacttataa aacttgctga tagattgcac aacatgagaa cattaaaata
     5281 catgcctcca gaaaaagcaa agtataaagc aaaagagaca cttgaaattt atggtggtat
     5341 agctcataga ttaggaattt ccaagataaa gtgggaacta gaagacagag cattaagatt
     5401 tatggaccca gaagggtatt atgatttagt tagtagagta tcaatgaaga gaagtcaaag
     5461 agaggattat attcaaggaa ttgttgaact tcttaaagat aagtttaaag atttaagtat
     5521 accatgtgat gtatatggta gaccaaagca tttttacagt atatatagaa agatgcaaaa
     5581 aaaacataag acttttgaag agatttttga tttaacagca gtaaggatat tggttgataa
     5641 tgtaaaggat tgctatgcag tacttggtat agttcataca ttatggagac ctattccagg
     5701 aagatttaaa gattatatag caatgccaaa acccaatatg tatcaatcat tacacaccac
     5761 tgttgtaggt cctgatggag aacctttaga gatacaaata agaactcatg agatgcataa
     5821 tatagctgaa aatggtatag cagcccattg gaaatataaa gaaggtatct ctagcaaaga
     5881 gtcaaaggtt gaagaaaaac ttcaatggtt gagacagatg atggagtggg aaaaagattt
     5941 aaaagaccca caagagttta tggatgcact taaagaagat gtatttaata gtcaagttta
     6001 tgtatttacg ccaaaaggag acgtaattga attaccagca gaatcaacac ctatagattt
     6061 tgcctataga gttcatacaa atgtaggtaa taaatgtgta ggagctaaaa ttgatggaag
     6121 aatagttcct attgattata agcttcaaaa tggtaatatt gtcgaggtac ttacatcatc
     6181 taattctaat ggaccaagta gggattggct taatatagtg aagaccccaa atgcaaaaag
     6241 tagaattaga caatttttca agaaggaaag aagagaagaa aatgttgacc gtggaaatac
     6301 aatattagaa agagaattta agaagcatga tataccacaa aaagaccctc ttgttgaaaa
     6361 atatatggca ttagttgcta agaaatttaa tcagccaagt gttgaggact taattgccac
     6421 tgtaggttat ggcggtataa tgtcatctca agtggtatct aaggtaaaag acctatattt
     6481 aaaagaggtt aaaaaagcta aaaaggaaca acagatacaa gaagaagatt taaataaaca
     6541 caatataagt gaaacagaat acagtaaaaa gagaaagaaa aacgcatcac aaggaattat
     6601 agtaaaaggg cttgacaata tcttagtaag atttgctaag tgttgtaatc ctcttccagg
     6661 agatgaaatt gtaggatata ttactaaggg taggggagta gccatacaca gagctgattg
     6721 cccaaatgca aacaataatg gtgatttctt ccaaaataga ttggtggagg tttcatggag
     6781 ttcagcagga aaaggtaagt ttgaagcgga aattcaaata aaagctattg acagaagagg
     6841 aattgttaat gaaataactc atattgttgc aatggataaa cttagtttaa acggaataaa
     6901 tgcaagaaaa ggtaaggata gtattgtaag tataaactta cttgtagagg ttaatgacac
     6961 agaggaatta aaaattctaa tgaaaaaatt aaaagcaatt cctggtgtag aaagtattta
     7021 tagaatgaca aattagagtt aaaataatag ggaggaaact atgagagcag tagtgcaaag
     7081 agtatcttca tctaaagtta cagtagatga aaacactata ggtcaaataa ataaggggtt
     7141 actagtattg ttaggagtga ctcatgatga taaatcaagt gatgtagatt atatgattga
     7201 taaaatattg aatctgagaa tttttgaaga tgaaaatgac aagatgaatt tatctctaat
     7261 ggatataggt ggagagcttt tagtagtatc tcaatttact ttatatggag attgcagaaa
     7321 aggaagaaga cctggattta gtaatgcatc tagaccagaa cttgcaaata acctttatga
     7381 agaatttgta aaaaaagcaa aagataaagg tgtgacagtt ggtacaggtc aatttgcagc
     7441 tcatatgatg gtagagttaa ctaatgatgg tccagtaaca atactattag actcaagtaa
     7501 atctttttaa gggggaaaac tttaatgatt ataaaaaaat ttgtagatag ttattttgga
     7561 gtaaatactt atgtattagg tgatgaaaag actaaaaaat gtgcagttat agacccaggt
     7621 ggaagtttag tagagacatt aagttttgta aaagaaaatg aattaaatat tgaatatatt
     7681 atccttactc atgggcatgg agaccatata ggatatgtta aggatattaa agaaaaaact
     7741 ggtgctaaag tagttgcaca tgttgatgaa aaagaattat taaatgataa aaataaaaat
     7801 ctaagttata caatgagatg tggagcgcaa gagtttgatg cagatatcta tgtgagtgat
     7861 aaagacaagc ttgaacttgg tgaattaaaa atgactttct tgcatactcc aggacatact
     7921 caaggatgta tgtgtataag agttgaagat gaaatgttca caggagatac tttgtttgct
     7981 ggaagtattg gtagaactga tttatatagt ggagacttta accaaatgga aaaatcactt
     8041 aagaaattat gcaaatatga agatgctgtt agagtatatc ctggacatgg accaaccagt
     8101 actctagggg ttgaaaagaa gacaaatcct tacatgtaat tttatattag tatttaaaaa
     8161 agtataaaat atttaataac tatattagtg agggggttct tctactgtta aatagaagga
     8221 gttaagttga tgttgtatgt ttttttaaaa ggacatgatt ataagtatga agtagcagaa
     8281 cttataaaat tgtttaccag tgaatttaag tttaaagact ctaatgctaa caataatata
     8341 gagtcaaagc atttaataaa tagattgatt tatgagaatg gtgtattatt ctcaagtact
     8401 gaatactatg aaaatggaaa tttaaagtac gagtcaatac agaatataga aaatatgaat
     8461 cttgaatttt tagatggatt tgatagtagc aaaacgattg aagaatttct taatagacta
     8521 gatagtgaaa gtaagaggaa ctttaaaaag ctgtgtaaag aaaatatcaa aaaaagcatg
     8581 tttgtagtgc ttaaacaagt atttaattct tatgtaccat ggggaatttt gactggtatt
     8641 agaccagtca aaattgtcca caatttaatg gataaaaagc tagatgataa tagcataaga
     8701 accattctaa aagataatta ttttataata gataagaaaa tagatttagc tcttgaaata
     8761 gctaaaagag agcgattatt tatgtatcct atagataaaa ataaaatttc tctttatgta
     8821 agtattccat tttgtccaac tagatgtgta tattgttcat ttccttcaaa ttcattaaaa
     8881 caatttggac atttaaaaag agaatatgtt tataaattga ttgaagaaat taaggggttt
     8941 gccaaagtta ttaaggagac taagaaagaa gttgaaactc tatatattgg tggtggaact
     9001 ccaactacac ttgatgagga agaattagat ttgcttataa attcattgtt caatgaatta
     9061 gatttaagta aaattaaaga atttactgta gaagcaggta gacctgacac tattacagaa
     9121 caaaaactaa gagtgttaaa aaaacacaat gttagtagaa ttagtataaa tcctcaaact
     9181 atgaatgatg ataccttggt aaagattgga agagagcata gagtatctga tttagtagat
     9241 tgctttaata tggctcgtag aatgggattt gataatataa atatggatat aattttaggt
     9301 cttatagatg aagaattaaa tatggtaaag aacacattgg aggagataaa aaaactttct
     9361 cctgaaagcc ttacagttca tacattagcc ataaagagag catccaactt aaatataaat
     9421 atggataggt ataaagaaca tctaacacaa tatgaagaaa tggtaaaaat gatagattta
     9481 tcaatgaaat atgcaaaaga tatgggatta aatccatatt atatgtacag acaaaaacat
     9541 atgttgggaa atttagaaaa tataggttat gctaaagaag gatatgagtg catttataat
     9601 atacagatta tggaagaaaa acaaagtaac tatgcattag gtgcaggtgc tatatcgaaa
     9661 tttgtttatg tagatgaaga tagaattgaa agagtagaaa atgtaaaaaa tgttgaacaa
     9721 tacattgaaa gagtagatga aatgataaaa aggaagaaag aagaggtata taaaaatgtt
     9781 gactaaagct cctagaggaa ctaaagacat aactccaaaa gaggcttaca aatggcgtta
     9841 tgtagaaaat aaatttagag aaatatgtgc tttatatgga tatgaagaaa tggtaacacc
     9901 tatatttgaa catacagaac ttttcaagag aagtgttgga gatactacag atatagttca
     9961 aaaagaaatg tattctttta aagataaagg tgatagagaa attaccttaa agccagaagg
    10021 tacagcaggt gtagtaagag catttataga aaataaatta tatgcagata ctcaaccaac
    10081 aaaattattt tatgtgacac cttgttttag atatgaaaga ccacaagctg gtagacaaag
    10141 acaatttcac caatttggaa tagaagcact tggaagtgat actccttcaa tggatgctga
    10201 aataatcgca ctagctgttc aattttttaa tgaagttgga ttaaatgatt tggtagtgag
    10261 tataaattca gttggctgtc cagtttgtag aaaagagtac aatgctttat taaaagagta
    10321 tcttgactca aaagctgata tattgtgtga tacttgcaat gagagaagag aaaaaaatcc
    10381 aatgagggtt atagactgta aaaatcctac atgtaaagaa aatatcaaag atataccatt
    10441 tattgcagac catttgtgtg atgattgtaa atctcacttt gataaacttc aagaatactt
    10501 aaaggaaatg aatataaact ttgtaattga taaaacaata gttagaggct tagattacta
    10561 tagaaaaact gcttttgaaa ttatatctaa tgatatagga gctcaaagta ctgtatgtgg
    10621 tggaggaaga tatgatggtc ttgttgaaca attaggagga ccaaaaggta taagtggaat
    10681 tgggtttgga ctaggaattg aaagactttt acttacactt gaaggtaatg gaattgaaat
    10741 agaaaaccct caatctacgg atatatttat agtaactata ggtgaagaag caaatacaag
    10801 aagctttaaa ttattaaaag acttgagaca aaatcatata agtgcagata aagaccatat
    10861 agagagaagt gttaaagcac aatttaaata ttcagataaa ataaattcaa aatttacaat
    10921 agtgattgga gatgatgagc ttaaaaatga cactgcgaca ttaaaaaata tgaaaacatc
    10981 agagcaaact actgtaaaat taagtacatt agttgaagaa ttaaagcaaa agctgtaaaa
    11041 taagaaagta tgatgttttg tgtattagat aaatatatag cattaaaatt aaataaaatg
    11101 gaggtagtat cttggaaact ttaaagggtt taaagagaac tcactactgt ggagagttga
    11161 gagaaaaaaa cataaatgaa gaagtcgtac ttatgggatg ggtacagaaa aaaagaaatt
    11221 taggtggtct tgtatttgtt gatttaagag atacaagtgg attatgtcaa atagtttttg
    11281 atacagatgt agataaagaa gcttttgaaa aagctgaaaa attaggggct gaatttgtaa
    11341 tagctgtaaa aggaaaagta tgcgaaagac aatctaaaaa ccctaatatg ccaactggtg
    11401 atatagaaat atttgcaact gaacttagac ttttaaacaa gtcagaaact ccgcctattt
    11461 atataaaaga tgatgatgat gtttctgaag cactaagatt aaaatataga tacttagatt
    11521 taagaaaacc atctatgcaa agaaatttaa aattaagaca taaagtaatg aatattacaa
    11581 gaaattactt atcaaacaat agattttgcg aaatagaaac accattttta atagctccaa
    11641 caccagaagg agcaagagat tatctagtac caagtagagt taatccagga aaattttacg
    11701 cattaccaca atcaccacaa ttatataaac aattattgat ggtaagtggt atggataggt
    11761 atttccaaat agttaaatgc tttagagatg aagatttaag agcagataga caaccagagt
    11821 ttacacaaat agactgtgaa atgtcttttg tcgagcaaga agatgttatg agtatgattg
    11881 aaggtttgct tgaagcgata tttaaagaag ttttagatgt agaattggca cttccacttc
    11941 ctaagatgac ttatgcagaa gctatgtcta aatatggttc agataaacca gatactagat
    12001 ttggatatga gttaacagat atatctgatg ttgtttgtaa ctgtggattt aaagtgtttg
    12061 ctgatgctac acaaccagga aaaagtgtta gaggtataaa cgtaaaaggc aaagctgacg
    12121 actttactag aaaacaaata tcatcactag aagaacatgc taagacatat agagcaaaag
    12181 gtcttgcttg gatgaaagta ggacaagaag gagtaacttc tccaatagca aaattcttta
    12241 atgaagaaga aatgaacgct atacttacaa aaatgaatgc tgaagtagga gatttacttt
    12301 tatttgtagc tgataaaaac tcaatagtat ttgatgcttt aggacaagtt agacttgaag
    12361 tagcaaatag gcttaatcta ttggataaaa atgtatataa cttactatgg gtaactgagt
    12421 tcccagtttt tgaagaagat gaagaaacag gcacattttc agcaatgcat cacccattta
    12481 catcacctat ggatgaagat ttagacaagt tagaagaagg tgataagtca tctttaagag
    12541 cgaaagctta tgatattgta ttgaatggtt atgaaatagg tggaggaagt gttagaatat
    12601 caaattctga tattcaatca agaatgttta aagcacttgg tttcacagag gaaagagcta
    12661 atgaaaagtt tggttattta ttagaagcat ttaaatatgg tactcctcct catgctggtc
    12721 tagcttttgg tcttgataga cttgttatgt tacttgcagg aactgataat ataagagaag
    12781 ttattgcatt ccctaaaaat caaaatgctg tttgccctat gacaaatgct cctacattgg
    12841 cggaagatga gcaattagaa gaattaagta taaaagttga tataaaagat aatgaataat
    12901 atgtagttgc aagaaactaa aatgtaaaaa gtctggatat aaagtatatt tatattcaga
    12961 ctttttttat tagaattatt ttatattttt tctctatata ttttcataaa ttatccttgt
    13021 attctcagcc aatataatta aaatactatg tttaaaaatt ttacataaac ttgattgttt
    13081 tagaaattac ttctgta
//
LOCUS       DACTGW010000099        13077 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.099, whole genome shotgun sequence.
ACCESSION   DACTGW010000099 DACTGW010000000
VERSION     DACTGW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.099"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            10..1443
                     /locus_tag="I9573_002151"
     CDS             10..1443
                     /locus_tag="I9573_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HAT4796770.1"
                     /translation="MLTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNFVQFRYF
                     IEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGGPGAVFW
                     MWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFSILITIC
                     YGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSEIIVPVF
                     AGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRGLFSNEA
                     GMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSSGATGIE
                     LAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLNVFRVFV
                     VGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQKKSGIKD
                     PVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1663..2124)
                     /locus_tag="I9573_002152"
     CDS             complement(1663..2124)
                     /locus_tag="I9573_002152"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAT4796771.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGRYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2230..3423)
                     /locus_tag="I9573_002153"
     CDS             complement(2230..3423)
                     /locus_tag="I9573_002153"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HAT4796772.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3446..4111)
                     /locus_tag="I9573_002154"
     CDS             complement(3446..4111)
                     /locus_tag="I9573_002154"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAT4796773.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(4145..5248)
                     /gene="ribD"
                     /locus_tag="I9573_002155"
     CDS             complement(4145..5248)
                     /gene="ribD"
                     /locus_tag="I9573_002155"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAT4796774.1"
                     /translation="MNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNIIGK
                     GYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKRVVI
                     STLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKSAVS
                     LDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQEKVS
                     HPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSKNNL
                     VDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPTFVS
                     GEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5380..5501)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5690..8173
                     /gene="recQ"
                     /locus_tag="I9573_002156"
     CDS             5690..8173
                     /gene="recQ"
                     /locus_tag="I9573_002156"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAT4796775.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDLKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GANILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVMLDSYLENLKNNIIAE"
     regulatory      8261..8372
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8514..9815
                     /gene="thiC"
                     /locus_tag="I9573_002157"
     CDS             8514..9815
                     /gene="thiC"
                     /locus_tag="I9573_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAT4796776.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            9869..10063
                     /gene="thiS"
                     /locus_tag="I9573_002158"
     CDS             9869..10063
                     /gene="thiS"
                     /locus_tag="I9573_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAT4796777.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            10050..10859
                     /gene="thiF"
                     /locus_tag="I9573_002159"
     CDS             10050..10859
                     /gene="thiF"
                     /locus_tag="I9573_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAT4796778.1"
                     /translation="MEVVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPI
                     KTDMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSN
                     IAISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLN
                     KRDIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDS
                     NIITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            10922..11692
                     /locus_tag="I9573_002160"
     CDS             10922..11692
                     /locus_tag="I9573_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAT4796779.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            11709..12812
                     /gene="thiH"
                     /locus_tag="I9573_002161"
     CDS             11709..12812
                     /gene="thiH"
                     /locus_tag="I9573_002161"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAT4796780.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYEFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            12813..>13077
                     /locus_tag="I9573_002162"
     CDS             12813..>13077
                     /locus_tag="I9573_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAT4796781.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINS"
BASE COUNT         4868 a         1569 c         2225 g         4415 t
ORIGIN      
        1 ttaaatggta ttttaacgag tattaacaat gcagtagtat ttgtaaacaa ttacttatgg
       61 tcgtatatct taattataat gttagttgta attggtattt actttactat taaaacaaat
      121 tttgtacaat tcagatattt tatagaaatg tttagacttc tgggtgatgg tacagcgaat
      181 aaaaatgcaa aaaaggaagg aaaaatctct tcatttcaag cattttgtat aagtacatct
      241 tcaagagttg gaactggaaa tatagctgga atagcaattg cagtggtagc agggggacct
      301 ggtgctgtat tttggatgtg gcttatagca ttaattggtt ctgcatcaag ctttgttgag
      361 agtacattag ctcagattta caaagtgaaa gatggtcaag cttttagagg tggaccagcg
      421 tattatatgg aacaaggtct aaataaaaaa tggatgggta ttttattctc aatacttata
      481 actatatgtt atggttttgt atttaatgca gttcaagcaa atactgtttc acttgcattt
      541 aataatgcat ttggaataag taaaatggct atgggaatag gtcttggaat tttaactgca
      601 atagttattt ttggtggagt gcatagagtt gcaaaagttt ctgaaataat agttcctgta
      661 tttgcaggat tatatatatt agttgctttg gctatagttg taatgaatat aacagagata
      721 ccaagtgtta tagcattgat agttgaaagt gcatttgatt ttaaaggaat ggcgattggt
      781 acatttatgg gagttgtaat gactggagtg aagagaggtc tattttctaa tgaagcaggt
      841 atgggtagtg ctcctaatgc tgctgcaaca gcacatgtaa ctcatcctgt aaaacaaggt
      901 ttaattcaat ctttaggtgt ttttacagat actataataa tatgtagttg tacagcattc
      961 atggtattat tatattcagg ttatagtagt agtggagcaa ctggaattga attagctcaa
     1021 gaagctttaa ctcatcatat aggaccagtt ggaaatatat ttatagcagt ttgtatattc
     1081 ttgtttgcat ttagttctat agttggtaat tactattatg gtgaatcaaa tatggagttt
     1141 atgagtggaa gtaagactaa acttaatgta tttagagttt ttgttgtagg catggtttta
     1201 tttggttcac ttactcaagt agatgtagtt tggaatcttg cagacttatt tatggcattg
     1261 atggcgataa taaacttaat agcaatagca cttttaggaa aatatgcatt tattgcttta
     1321 aaagattata caagtcaaaa aaaatcagga ataaaagacc ctgtgtttat tgcagatgaa
     1381 atagaaggcc tagaaaaggt atcagagtgg catagggaat tatctgagga acatttagga
     1441 taaaaaatag tataaaatta taaaaccatt ttgaaagact agagtatgta tatgctttat
     1501 atgaatataa tattgaatta atttatatta ctggaatttt taattccagt aatatttttt
     1561 tattatataa tttagcttta ttaaaagttt ttattattaa aaatagctta tttatcgtaa
     1621 agtgaatgat attggttttt taattattat gaatgttggt atttaattta aagacttaaa
     1681 taaatttgcc atttctagcg cattcatagc acaatcataa cctttatttc cagcttttgt
     1741 acctgctctt tcaatagctt gttcaatatt ttctgtagtt acgattccaa atataacagg
     1801 taattcactg tctaaagata cttttgctat ccctttagag acttcactac atacataatc
     1861 ataatgagaa gtagcccctc ttataacaca gcctaaacag attatagcat catacctacc
     1921 acttttagcc attttttgag caacaagagg aatctcaaaa gctccaggta cccaaactat
     1981 ttctatattt tctggagaaa cgtcatgtct taaaagacag tcttcagctc ctgataaaag
     2041 ttttgatgtt ataaattcat taaaacgaga atttattata cctattttta aatcttttcc
     2101 aataagtttt ccttcatata tcatttttat ttcctccaat tttatttatg ttatagattt
     2161 atatttataa atatctttaa ttttaattat aaaaaactac ttagtagaaa aatatcatca
     2221 tataatcatt tatataatat ctaaaagatg tcccatttta tcttttttag tttttaaata
     2281 tagcttatta aagtcattaa tttcttcatt aatagggatt ctattttcga tttttattcc
     2341 atatttctct aattgattta ttttatccgg gttattactt aaaagattta tactttttat
     2401 gtttaaattt tttaatattt gagcagccat atagaaatct ctcatatctt cttcaaatcc
     2461 aagcattaaa tttgcttcaa cagtatcgta accttcttct tgaagtttat aagccttaat
     2521 tttatttaaa aggccaatac ctctaccttc ttgcttcata taaattaaaa ctccacttcc
     2581 attttccaca attttcttca tagaagattc tagctgtaat ccacaatcac attttagaga
     2641 gtggaacaca tctcctgtta aacattcaga atgaagtcta actagagtat tttctatatt
     2701 tatatttcca taagtaaggg caatgtgttc ttcatttgaa taagtatctc tatatcctat
     2761 gatttcaaaa tttccatatt ttgttggaag gaaagcacta gatacttttt ctatagtagt
     2821 ttcatttatt ttgcggtatt ctataaggtc ttttatagtg atgattttta aattatgctt
     2881 ttctttgaaa atcattaaat catcaactct tgccattgta ccatcatctt tcattatttc
     2941 acagataaga ccaatctctt taaaacctgc aagtctagca aggtctactg aggcttctgt
     3001 atgtccatcc ctgacaagaa ctccaccagt ttttgctatt aaaggaaata tgtgaccagg
     3061 cgttttgaaa tctttacttg tagaattttc atcacagaac aattttacag tttcagctct
     3121 ttcatatgca gatataccag ttagagtatc tgctccatct atagtaacag taaaggctgt
     3181 ttgaaatgta tctgtatttt ctctaaccat aagaggtata ttaagacttt taaatttttt
     3241 ttcagtggca ggtagacaaa ttagacctct accatatgta gccataaaat ttattgattc
     3301 aggagtagcc atttcagctg ccatcaataa atctccttca ttctctctac tttcatcatc
     3361 aactacgatt accattttcc cattttttat atcttcaata gcttcttcaa tagtattaaa
     3421 catgtaattc actcctttat tatttttaaa aaccattttt aaataaaaaa tctttatcta
     3481 tatttgattt atggttacta ctattagaat ttaattcctt gtagttatta gccataaaat
     3541 tattaacata ctttccaatt acatcgcttt ctaaattaac aaaatctcca acattctttg
     3601 tcaagagaat agtgttaatt ttagtatgag gtataataga aaccttaaat atttcatcat
     3661 ctaaatatga tatagttaaa cttacaccat caatagcaac agaacctttg agtatcattg
     3721 aagataaaag ttttttatca ggtcgtatac ttactaaaac tgcattttca tctttttcaa
     3781 atttgcagat agttccttta ccatcaacat gacctgttac tatatgacca ccaaatcttg
     3841 aagaaagact catagctctt tcaagattta ccttatcatt tgcatttaat aaacctaaat
     3901 ttgtggaacg aattgtctcc atcataacat cggctgtaaa ttcatttttt ccgaggtttg
     3961 aaacagttag acaaactcca tttacggcta tactatctcc aagatttgtg ccatctaata
     4021 ttttatttga tagtatggtt acttttccac tcttaccatt agcagtaatt tttctaagta
     4081 ttccaacttc ttctactatg ccagtaaaca tcctaacctc ctatatgaat gtattaacaa
     4141 aaatttaatt taaaacatct gattctataa gaatatcgcc atctatcaaa gttacattat
     4201 tgattgttaa ctgtgttgaa tctgacaaat gatttattcc ttctccagaa acgaatgtag
     4261 gtgcatcttt accacctata atcttgggtg atagaaataa tttgacttta tcaactaact
     4321 tatttctaaa gagactgtca ttgagagtag caccaccttc aactagaaca gaatcaatgt
     4381 ttaactctcc taatttatac attacaaatt ccaaatctac taagttgttt tttgaaggag
     4441 aaattattgt ttcaacatta ttttctttta acatagataa ttttatactt tctttgcaac
     4501 atgtgaaaac tatagtttta ctggttttat ctttaaccaa attactatta agtggcaact
     4561 ttaggtgtgt atctattact attcttgtag gatgagacac tttttcttga ttgagcctgc
     4621 aagttagtaa aggattatcg ttcaaaactg tatttatgcc aaccattata ctttggtatt
     4681 tatttctgta tttatgagtt agataccttg atgattcatt agatatccat tttgattcta
     4741 gtgattttgt agcaatttta ccatctagac ttactgctga ttttacaata caaagaggtc
     4801 ttttgttttt tatatgataa aaaaatgcct catttaaatc tcttgcttca tcttctaata
     4861 ttcccacagt aacatcaata ttgttatctt ttaatttttt tactccatta ccacaaacaa
     4921 gtggatttgg gtctaatgta gatattacaa ctctcttaat cttattttgt ataattttat
     4981 ctacacaagg aggcgttttc ccataatgag aacatggttc taagtttaca taaagagtag
     5041 agtcttttag actttgttta gcactgttta tagcatttac ttcagcatga ttagagccaa
     5101 atttctcatg atatcctttg cctattatat tactatcttt aacaattaca cacccaacta
     5161 atggatttgg atttacaaag ccttctccat ttttagccaa ttcaatagct tttttcatat
     5221 aataaatatc tttttctttt tgattcacaa tatgtacacc tcctgtttaa ttggataatt
     5281 tttaaacaaa aaaccctgag aattaactca gggtttagaa taatctacaa taaatgttta
     5341 ataaaagtag aagaaaattg ctctacaaaa acactcatta aatatcttct tccatccaga
     5401 ctttactgtc ggctttggaa tttaaccaaa tcaaccaagt tttaccttgg ctcgcgggct
     5461 ataccgccga ttgggaattt caccctaccc cgaagattaa gtattaaatt tatatttaga
     5521 atgtatcata aattatttta ctttgcaaac tattttttta aaaaatatat gttaatatat
     5581 aatatagaat gtctataaac tttgaaatac cacgattgtg aatataaaaa taaaaatttg
     5641 aacataatca ggaaagaagt ctaattaatt taaattagga gagtaatata tgaataaaaa
     5701 acttttagaa atactatcaa aatactatgg atatacaagt tttagaaaag gtcaggaatc
     5761 tataataaat tctatcttaa gcgaaaaaga tgtgttagct attatgccaa caggaggggg
     5821 gaaatcaata tgttatcaaa ttccagcttt gattttagat ggaatgacaa tagtaatttc
     5881 tccacttata tcacttatga aagaccaagt agatgcctta aagactatgg gaattagtgc
     5941 tgcttttata aatagttcat taagcagcaa agaatttaat gaaatattaa ataatattag
     6001 aaaaaataat tataaaatac tctatattgc tccagagaga ttagatgcac aagagtttat
     6061 tgagctaata aataataaca atgtaagtca agttgcgata gatgaagcac attgtgtatc
     6121 tcaatggggg catgatttta ggttgagtta tagaaggatt tctgatttta taaaaaattt
     6181 gcctaaaaga cctatagtta cagcatttac tgcaacagct tcagaagagg taagaactga
     6241 tataataaat ttactgtgtt tagaaaatcc agattattat ataactggat ttgatagaga
     6301 gaatttatca ataaatatag taaaatcatc tagtaaaaat aagtatattt tagattatat
     6361 tcaaaatcat aaaaatgagt ctggaattat atatgttgca actagaaagg aagttgaaaa
     6421 tatatataat ggtttatcta agagaaatat atctgtatca aaatatcatg caggtttatc
     6481 taaaaatgaa agaagtaaaa atcaagaaga ttttataaat gataatgtgg atataatggt
     6541 agcaacaaat gcatttggaa tgggtattga taaacctaat ataagatggg ttatacatta
     6601 caatatgcct caaagtatag aaaactacta tcaagaaata ggtagagctg gtagggatgg
     6661 agaaaaaagt gagtgtgtac ttttattttc tccaggagat gtacatatac aaaaatattt
     6721 aattgatatt ggagttgaaa atcctgaaag aaaattattt cagcataaga agcttcaata
     6781 catgattgac ttagtttaca gtaattcatg ctatagaaag acaatattga gttattttgg
     6841 agaaaattat atagacaatt gcaataattg tagcaattgt ttagtagagg gtgaaatagt
     6901 agataaaact atagatgcac aaaaagtcat ttcatgtgta gctagaatga aaagaagtta
     6961 tggtgttact actattgtag atgtacttag aggttctaag aataaaaaga ttttacagct
     7021 tggattaaat actttgtcta catataacat tatgagagat tattctagtg aagacttaaa
     7081 gaattttgta aatacattgg tttctcatgg ttttttagat ttagttgaga ctctaggcaa
     7141 tggaaataga ggtagttttc ctactataag attaaatgag atgtctatga aagttttaaa
     7201 aggtgaagtg aaagtagagt ttaaagaaat tgttatgaca aaatcattgg aagtagagga
     7261 tgagttatat aatgctttaa gagaacttag acattcaatt gcaagtgaag aaaggatagc
     7321 tccatatatg gtatttggag atggtacact tagagccatg tctagtagtt atccaattaa
     7381 taaagaagaa atgctagata tctctggagt aggagaaatt aaatatcaaa aatatggtag
     7441 agattttgaa gcagtaatta aagaatatgt cgaaaaaaat aatataaata aaaaaataga
     7501 tgaaaaaaat gtcgatttaa agaacgttaa tagtgatttc ttagaggtta atacagataa
     7561 agttttatat gaaaaactgt tagtcattag agaagaatat gcaacaaagg aaaaattacc
     7621 tgcatatatg attttagcca ataaggcttt gaaagaaata agtggaagat atccacttga
     7681 tgaagaacaa ttaaaagata tttctggcat aggtggagta aaatttgaaa aatatggagc
     7741 taatattttg gcagaggtta ataaatatgt aaaagaaaat aatattaatg ttatttggga
     7801 gaaaaagggc aaaaggaagt taatattaga tggagaaagt agaaaaaata atgaaatagc
     7861 attagatttg ctaaatcaag gaaaagatat acaaagtatc agtcaagaaa tagaagtttc
     7921 attgtcaacc attattggat atgtgcatga ctatgtaaag gatggacata gtatatcatt
     7981 tgaattagga ctagaaaatt tttataatga agataaaaag aagatgattt tagaagtttg
     8041 cgaaaaagtt ggctatgata atttaaataa cataaaaaga aaacttccag attcaataaa
     8101 gtatgaaaat ataagagctg tgatgcttga ctcttatctt gaaaacttaa aaaataatat
     8161 tatagcagag taatttttaa cttaataaaa ataaaatttg tattattgtg ttgaactaat
     8221 aattttgctg tgttatactg aatttgtaaa aataatgaaa aattagagct aggggcgcta
     8281 gaattggcta gctgagagat aaagccgtaa cttacaaacc ctaataacct gatcaagata
     8341 atgcttgcga agggaagctt tttaatataa ttttattata taaataggca aattattcaa
     8401 tgttaggaga aatttgtcta agtgtatata cgattataag agtgctttcc aaacgaggaa
     8461 aagcactttt tttattttca aaaactataa ctttaatctt ggaggattgt aatatgaact
     8521 atacaacaca aatggatgct gctagaaaag gcataataac taaagaaatg gaaatcgttt
     8581 ctcaaaaaga acaggtagat gtaaatgaat taagagaatt aatagcaaat ggacaagttg
     8641 ttataccagc taataaaaat cataaatctt taagtgcaga aggtgtaggt aaaaatttaa
     8701 gaacaaaaat aaatgtaaat ctaggaattt caagagattg taaggatata gaaaaagaac
     8761 ttgaaaaagt tagagtagca atagacatga aagctgaagc tataatggat ttaagtaatt
     8821 atggcaaaac tagagaattt agagaaaagg ttgttgaaat gtcacctgca atgataggtt
     8881 cagtacctat gtatgatgca gtaggatatt tggaaaaaga attaaaagat ataacagaag
     8941 aagagttttt aaatgtaata agacaacatg ctattgatgg agtagacttt attactatac
     9001 atgcaggtct tacaagaagt gtttgccaaa aaataaaaaa tcatgagaga ttgactcata
     9061 tagtatctcg tggaggttct ttattgtttg catggatgga attaaataat aaggaaaatc
     9121 ctatttatac taattttgat aaaatattgg atatatgtga agaatatgat gttacattga
     9181 gtttaggaga tgcttgtaga ccaggttgta taaaagattc tactgatggt gttcaaattc
     9241 aagaacttgt agtattaggt gaacttacta aaagagcatg ggaaagaaat gtacaagtaa
     9301 tgatagaagg tcctggacac atggctattg atgaaataga agcaaatgta gtattagaaa
     9361 agagattatg ccatggagca cctttttatg ttcttggacc attggttaca gatatagcac
     9421 caggttatga ccatataaca agtgcaatag gaggagcttt agcatgtgct aaaggagtag
     9481 attttttatg ttatgtaact ccagcagaac atttaagatt gcctaattta gatgatatga
     9541 aagagggtat aatagctgca aagatagctg ctcatgcagg agatatagct aaaaatgtta
     9601 aaggtgctcg tgaatgggat aataagatga gtaaagctag agctgattta gactggtgtg
     9661 agatgtttag acttgcaata gaccctgaaa aagctaagag atacagagat gaatcaactc
     9721 ctactcatga ggatagttgt actatgtgtg gaaaaatgtg ctcaatgaga acagttaaga
     9781 aaatattaaa caatgaggaa cttaatctaa tataattttt ggctagtatg actggaataa
     9841 aaagaagctg aaaagaggag gtgatttagt gaaggttaat gggaaggaaa ttgaatttga
     9901 aaaagatttg actgttatag atttattaaa taaatataat ttaaaaagtg atagagttgt
     9961 agttgaagtg aatttagaga taattgaaga atctaactac aatacatatg tattaaaaga
    10021 cgaggacatt gtagagctta ttagctttat tggaggtggt taaaataaaa atatttgtaa
    10081 atgaattatt aacagatgtt gaggaaaata caactgctta taaagttaga gatagctata
    10141 acaataaatg tgatgtattg gtgttaaatg gatatccaat taaaacagat atgccccttt
    10201 gtgaaaatga taaattaaca cttatacaaa aaggagttaa gccatcactt gatgaattgg
    10261 aaagcttgtt gattgctaga catactccaa atatacataa taaattaaaa aaagggaaag
    10321 ttgctatatt gggattgggg ggacttggtt ccaatatagc tatctcttta gctagaatag
    10381 gagtgggaga acttttactt atagattatg atgttgtaga accatctaat ttaaatagac
    10441 aacagtattt tatagatgat ataggaaaac taaaaactga tgccatgata gaaaatataa
    10501 aaaaaataaa tccatttata aaattaaata aacgagatat ttttttaaac aagaacaata
    10561 tggatactat taaggattca gatttaataa ttgaggcctt tgatgatgct tcatgtaaag
    10621 cacaggtatg taattatgta ttaataaatt taaaagataa atatttaata gcatcatctg
    10681 gtatggcagg ttactatgat tcaaatataa taactactaa gaagataaaa gacaggtttt
    10741 atatatgtgg ggattttgtg aatgaagcta aatttggtga agggcttatg gctccaaggg
    10801 ttgccatctg cgcaaatcat atggcaaatt tggcaacgca aatacttata gatatgtaaa
    10861 tattaaaatt agatataaaa aagattgaat attaaaaaaa tttaacgtgg agggaaataa
    10921 tatggataaa ttggtacttg gtggacatga atttaatagc agattattgg ttggaacagg
    10981 taaatatggt tcaaataata tattgcctga ggtaataaaa gaaagtggaa gtgaaataat
    11041 aacaatggca cttagaagag tggatttaga caataaacaa gaaaacatat taacgtatat
    11101 tccaaaagag atgacaatac ttccaaatac atcaggagct actaatgcag aagaagctgt
    11161 aagaatagct agaatatcta gaaaaatggg atgtggagat tttataaaaa tagaagtaat
    11221 atcagatact agatatttac ttcctgataa tgaagaaact ataaaagcta caaagatact
    11281 tgctgatgag gggtttatag ttcttccata tatgacacca gacctttatg ctggaagaag
    11341 acttatagaa gctaatgcag ccgcagttat gccacttgga gctccaattg gttcaaatag
    11401 aggtcttcaa atgaaagaaa tgataagaat aatgatagat gagttggata taccaataat
    11461 agtagatgca ggtattggaa aaccatctca agctatggag gctatggaaa tgggagctga
    11521 tgcagtactt gtcaacactg ctatagcttc agcaggagac cctgtacaaa tggctagagc
    11581 atttaaatta gctgtagaag gtggaagaga agcatatatt gcaaaaacag gaaatgtgtc
    11641 agaatttgca aatgcatctt ctcctttaac aggtttttta ggcaatttat agaaagtggt
    11701 gattggtgat gagtttttat gatgtaatag aaaaatatag ggattttgat tttgatggat
    11761 atttaaataa tgttactgat aatgatgttt taagaagttt atcaaaagac aagcttgaag
    11821 attttgatat attaaatttg ctttctaaaa ctgcagtaaa acatttagaa gatatggcac
    11881 aaaaagcaca taaattaagt gttcaatact ttggtaaaac agtatgttta tatacaccaa
    11941 tgtatatagc aaattattgt gttaatcaat gtgtttattg tagctataat ataaagagtg
    12001 gaataaaaag aaaaaaatta actatggatg aaattagaga agagggagaa gcaatttcaa
    12061 aagaaggttt caaacatcta cttgttttga ctggagaaag tagttttcac tcaagtgtag
    12121 aatatatagg tgaggctata gaaatattaa gagagaaatt tccctctata ggtatagaag
    12181 tttatccaat ggaagtagaa gaatataagt acattgtaga taaaggtgtt gaaggattaa
    12241 cagtatatca agaaacttat gacgaggaaa tatataagag agtacatata aaaggcccaa
    12301 aatcaaatta tgaatttaga ctagatgctc ctgaaagagg ggcaaaagca ggtatgagga
    12361 ctttatcaat aggtgcactt ttaggtctaa atgattttag aaaagaaact ttttttacaa
    12421 tattgcatgg gaaatactta aaaacgaaat atcctcatat tgagctttca tattcaacac
    12481 caagaatgag accttttaaa gggtgttttg aggaattggt agatataagt gatactgatt
    12541 tagttcaagc tatggtatgt atgagattat ttgaccctca tgcagctata aatatatcca
    12601 ctagagaaaa tttagaaatg agaagtcata taataccttt aggagtaact aaattgagcg
    12661 caggtgtatc tactgatgta ggaggtcatt ctcaagatga acatgatact gctcaattta
    12721 agataaatga tgaaagtact gttaaagatg tggagaaaat gctaaactca attggttatc
    12781 agcatgtgtt taaagattgg gaaagatttt aaatgtatct gataacaaac agaaaattat
    12841 gtagtgagga acgatattta gaagttatta aagagtctat attgagtggt gtagaaaata
    12901 taataataag agaaaaggat ttggaatatc aagagctaag aaaattatat atgaaaataa
    12961 aaactaaaat caattgtata gattttcaag agcaaatatc agatgaaagt ttgaaaacta
    13021 atataaatca aaaagagtgt agaaataaat tcaaggttaa ttttataata aatagta
//
LOCUS       DACTGW010000100        12995 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.100, whole genome shotgun sequence.
ACCESSION   DACTGW010000100 DACTGW010000000
VERSION     DACTGW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12995
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.100"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            71..952
                     /locus_tag="I9573_002163"
     CDS             71..952
                     /locus_tag="I9573_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT4796782.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1160..1969)
                     /locus_tag="I9573_002164"
     CDS             complement(1160..1969)
                     /locus_tag="I9573_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HAT4796783.1"
                     /translation="MFIDESGRADINHEDPFTLTGVIFEYKYAVSQGDYVCSLRKEMD
                     AFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDELPLFLSRLECTIISVTVD
                     KARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARIVIESRDDVSNLKVQKAFF
                     DVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIADILCNPLSRARRGLIEAN
                     PKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIVEPVGPWLEKMR"
     gene            complement(2178..4112)
                     /locus_tag="I9573_002165"
     CDS             complement(2178..4112)
                     /locus_tag="I9573_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HAT4796784.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKAINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLSV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSLEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4224..5237)
                     /gene="galE"
                     /locus_tag="I9573_002166"
     CDS             complement(4224..5237)
                     /gene="galE"
                     /locus_tag="I9573_002166"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAT4796785.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5262..6137)
                     /gene="galU"
                     /locus_tag="I9573_002167"
     CDS             complement(5262..6137)
                     /gene="galU"
                     /locus_tag="I9573_002167"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAT4796786.1"
                     /translation="MQMTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIE
                     EAIESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHY
                     IRQKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGV
                     QKIAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSIL
                     EKQTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENL
                     KDDLMDYMRKKVSEK"
     gene            complement(6329..6649)
                     /locus_tag="I9573_002168"
     CDS             complement(6329..6649)
                     /locus_tag="I9573_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796787.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6639..8630)
                     /locus_tag="I9573_002169"
     CDS             complement(6639..8630)
                     /locus_tag="I9573_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796788.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCDIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8752..11295)
                     /locus_tag="I9573_002170"
     CDS             complement(8752..11295)
                     /locus_tag="I9573_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HAT4796789.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGAKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQAKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11573..11907)
                     /gene="rnpB"
                     /locus_tag="I9573_002171"
     ncRNA           complement(11573..11907)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="I9573_002171"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12039..12716
                     /gene="srtB"
                     /locus_tag="I9573_002172"
     CDS             12039..12716
                     /gene="srtB"
                     /locus_tag="I9573_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HAT4796790.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKTSSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKNNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(12876..12950)
                     /locus_tag="I9573_002173"
     tRNA            complement(12876..12950)
                     /locus_tag="I9573_002173"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12916..12918),aa:Thr,seq:tgt)
BASE COUNT         4001 a         2286 c         1326 g         5382 t
ORIGIN      
        1 gtaaaaatat cttaaaggtt aacttctaca aatatataaa taaaaatacc tatgaaagga
       61 tgaacaaaaa ttgaatttat atcacttacg ctattttgtt acattggcac atttagaaca
      121 ctatacaaaa gcggcagaaa atttatctat aacacaacca agcttaagtc atgctatttc
      181 tttactagaa aatgaacttg gggtggctct atttgaaaaa gaaggtagaa atatagtcct
      241 tactaagtat ggaaaaatat ttttaaaaga tgtggaaaaa tcattagaaa ttttagattc
      301 aagtgtaaaa tcattaaaaa taactggtac tggtgaagga caaatagact tagcttttct
      361 tagaacactt ggtactgatt ttatcccaga catagtgcat aaatttttaa aatctaatcc
      421 tgctaaatca atagatttta aatttcatac tggtgtaact actgacatta ttcaaggtct
      481 taaagaaaga aaatatgata ttgcattttg ttcgaaactt gaaaaagaaa agggaattga
      541 atttatacct gttgccaaac aagatttagt actaattgta ccatatagtc atccactagc
      601 tgcaaaggat actatagatt taaaagaaac cataccttat cctcaaatag tatttaatca
      661 gagaagtggt cttagatata taattgatga tatgtttaaa aaaataaatc aacagcctaa
      721 tatagtctat gaagtagagg aagaccaagt aattgctgga ttagttgcaa aaaatttcgg
      781 aattgctgta gtcccaaata tgaatatgct tagctttaca aaagtaaaag taattcagat
      841 tattcaccca agttgggaaa gaaactttta cttagcattt ataaaagata gatatttgcc
      901 tcctgcaatt aaaaacttta aaaactttgt cattaaaaac gctcaattat aatttattaa
      961 taaaggggtt atttttaaat atcctctttt attcttttta taaaataaaa aaatctatac
     1021 ttttaaaagt atagattcct aactgacagt cggcatccga gtctcagtaa ttatcgttat
     1081 acaacagcaa ctggattcag ttcctgaata actaggttat ttacattata tcagattttt
     1141 ttaaatttgt caattgtatc taacgcattt tttcaagcca tggtccaaca ggctctacaa
     1201 taggaacttc tttaaatccc caatttctta tatcattcac actcgttgga gtatatattt
     1261 tgtttctgac agatttgaat attttgtttt tttctccata ttgcatacac tttggattag
     1321 cttctattaa ccctcttctt gctctgctaa gaggattaca taaaatatct gctatttcta
     1381 aaccttctct atagtcagca tcttcttttt tggcaattat aaaaccctta accttatctc
     1441 ttaattcttc atctatatct aagtatattg ttccattatt aaatacatca aaaaacgcct
     1501 tttgtacttt taagtttgag acatcatccc ttgactctat aactattcgt gcagattcta
     1561 cagtttcatt atttacaaat gaataaaaat tctgaagaac atgtataaaa gccacaacat
     1621 acggttcctt tgaaggtgca taatatttct ttaatctagc tttatctact gttactgata
     1681 ttatagtaca ctctaatcta gatagaaata aaggcaactc atcatagaat aacttaatct
     1741 gtgaatcatc aaacttctca aatgctcttg attttcttga tatatctgtc aaatgtattc
     1801 ctatgttact ggttccaaaa caatactctt taaatgcatc catttctttc ctcaaagaac
     1861 atacataatc gccttgactt actgcatatt tatattcaaa aattactcct gtcaatgtaa
     1921 atgggtcttc atgatttata tcagctcttc cactttcatc aataaacatt atatattttt
     1981 tctttataat cttactcacc ctcttaacat atgatgatag catctatatt cattttatat
     2041 gagttttaaa aatattccaa gatttttata taacttaaac ttaattttat actaaaaaat
     2101 tgagatgtaa aacaatcaag cttttattga ttgaatttca catctcaatt ataattattt
     2161 ttcaatatat atttttacta ttttgcaaga atatccatta atttatccat tactatataa
     2221 gatactccgc ctcctatttg ataaggagtt tcaacttttt tatttgcaaa tactctttgt
     2281 tgctctaatg ataatcctga attatccatt aaaactattg gtgaatgtaa ttttacagct
     2341 aatggtccag ctgataaagc atctactaaa gcatctgatt tactaactaa tacagcctgt
     2401 aaatcagtat ctccatagaa tttttctatt actttggcat tagtatctac tctactatct
     2461 ccatatattc tatttcctga aatatcttca gtagttatat cattcatttt tgctattact
     2521 gaatcgttaa ttgcacttgg tccacctata aagtatgcat tttctaattt tctttctgtt
     2581 agccatttat aagattcttc tgtaattcca tctttattga ctaatattat aggttgtttt
     2641 tcttccccag ctttagaagc tacagataaa gcatctgctt ccccatttcc agaaaccata
     2701 tatgcagttt ttatttcttc tcgatttcca agttcttcag ctattcttgt agcagtttga
     2761 tatctatctt gacctgctat tctctttgtc tctgtgttga aagtagtttt tatttcttgc
     2821 tctaatttag aacttattgc attttctcca cctatcaaga ttactgtttt aggtgctaat
     2881 aacttcattt cttcaacagt ttcagttggt aaattagctg tattagtaag tagtattgtt
     2941 gagtcatagc tacttgctaa aggagttgct gttataccat cagctattgc attaccattt
     3001 actaatatta cagtatctga actatcagca ttccagtttc caactgaact tatcttttta
     3061 gctgtatcaa atctatttgc acctataatt gtatcccatg taggatttat agttccatct
     3121 tcattaaaag tataatcttt cccatctatt ttttgagaac cagttagcat ctttccatct
     3181 ttgtctaaat agtaagactt accatctatt tgttgccatc ctgtctgcat tattcctgtt
     3241 ttgtcaaagt aatacttatt accatctatt tcttgccatc ctgttactga taaaccatca
     3301 gatcctagat agtatttctt tccattcttt tcttgccatc ctgtttgcat tactccatct
     3361 gagttaaagt aataagtttt tccgtctatt actttccatc cagatactct tgctccatca
     3421 gttcctaaat aatatttttt gttattaatt gctttccatc ctgtttgcat tactcctgaa
     3481 gcatcaaagt aatacatttt tccatttact tttagccaac tattcttttg gtatgttcca
     3541 tcagatttta cataatactt cttaccattt tctgttttcc atcctgtttg atttagattt
     3601 atcttatatt ttgccgctat ctttacatag ctgtcattac tataatctat aattacatct
     3661 gaacctacag gaacttggtc aaataaccat ctaacatctt tattgtgcat tcttatacat
     3721 ccaccagaaa tatgctttcc tatagaactc tcattattat ttccatgtat tccatatgta
     3781 tctccataag ttccttttaa tgctaatccc atccatctat ctcctaatgg atttcttgga
     3841 cttcctccag gtatattacc tttataatat ggtctatttt ttatcttgtt aactactttt
     3901 gttttccctg ttggtgtttc tgaccccttt ttacctgttg caactctaaa ttcttttaca
     3961 agtttattat ttacaaaata tcctagagta ttctttcttg aatttactat aatcacatgt
     4021 ttactggctg cgcttgattc tggtacgcca catgcaaata ctaaaactaa agtcagtgct
     4081 agtactaaat acttttttag actttttttc actataactt ccccctcttt tagaaacatt
     4141 actacatgtg tctaattata acataaaatt accaatacct tatatttaca aatttgttac
     4201 atttatacat ataagtatgt attctactct tcatacccat taggattcat actttgccat
     4261 ctccatgaat cttcacacat ctcttcaagt tcatacttag ctttccatcc aagctctttt
     4321 tctgcctttg aagaatctgc ataacacatg gctacatctc cagcccttct tcctacaatc
     4381 ttatatggta tttcttttcc acttgcttta gaaaatgctt ttactaaatc aagaacacta
     4441 taaccctttc cagttcctaa attataaaca acaagtcctg gattttcttc taatttttgt
     4501 aatgctttta catgaccagc agctaaatct aatacatgta tatagtctct aactccagtt
     4561 ccatcatgtg ttggataatc attaccataa acactcaatt ccttcaattt acctactgct
     4621 atttttgtta tataaggcat tagattgctc ggaactccat ttggttcttc acctattcta
     4681 ccacttttat gagcaccaac tggattaaag tatcttaaaa gagctatatc caatgactta
     4741 tctgcttttg atatatctac taacatctgc tctatcataa gtttagttct tccatatgga
     4801 ttggttacag aaagtgggaa atcctctaat ataggacaag tatgtgggtc tccatataca
     4861 gtagctgaag aactaaatac aaacttttta acaccatatt ctctcattaa ttcaaataaa
     4921 tttaaggtac ttattagatt attgctgtaa tattcaattg gtttttctac tgattcacca
     4981 actgctttta aagctgcaaa gtgtataatt gattcaatat tattttcttt aaatacaaca
     5041 tgcatttcat ctttatttct gatatcaata ttataaaatt ttactggttt tttagataat
     5101 tccttaattc tatcaaccac tattgagttg ctattactta agttatcaac tataacaact
     5161 tcataacctg attctaaaag ttctattgct gtatggcttc ctatatatcc tgctcctcca
     5221 gctactaaaa ctgccattgt aattacctct tttctaatat tttatttttc acttactttt
     5281 tttctcatat agtccattaa atcatctttt aaattttctc tctttagagc aaagtctatt
     5341 gttgcctcta aaaaaccaat cttatctccc acatcatatc ttctaccttc aaagttataa
     5401 gcataaatag cctcttgttt tccaagagtt tgaagtgcat ctgtcagttg tatctctcca
     5461 cctttaccag gtgtctgttt ctctaatata ctaaatattg caggagttat tatatatctt
     5521 ccaagtatag ctatatcaga tggagcttct tcaattgcag gtttttcaac catatcttta
     5581 actttatata ctctatcttc gatatgtttt acatccaata taccatattt attagtatct
     5641 tctttagcta tcttttgaac acctaataca gttgttttat actcattata agtatctatt
     5701 aattgtttta agcacggaac atctgaatct actatatcat cacctaataa tacggcaaaa
     5761 ggttcatcac ctataaaact ttttgcacag taaattgcat gtccaagacc ttttggctct
     5821 ttttgtctta tataatgaat atttaccata tttgatatgt ctcttaccat ttctaacatc
     5881 tctgtcttac ctttttgctc tagttctaac tctaattcaa ctgatttatc aaaatgatct
     5941 tctatagatt ttttatttct acctgttatt ataagaattt cttcaattcc cgattctata
     6001 gcttcttcta ttatatactg taaagttggt ttatctacta taggaagcat ttctttgggc
     6061 tgtgattttg ttgctggtaa aaatctggtt cctagccctg ctgctggtat tattgctttt
     6121 ttaactgtca tctgcatttt ttcacccttc ttcgtaaatt ttctattgta ccaaattaat
     6181 ataccatatt tatacttttt cttcaaactc tatattatcc caatatttaa tgctataaga
     6241 taatataagg tagatgaaat ttcatctacc tttagtttta taagttaata ttgtattgtt
     6301 tatattttat ccaaaactta tacaggtact aaatttcatc taagatgcct tttatatcac
     6361 tatctatatc ttcatctaga taagaatcta aatcttctaa gtcaacttct agttcttgat
     6421 atgtcatatc aacactgaca tcctcatcta tatccatttc tgatattaat tctcctaaaa
     6481 tactatttcc atcagaatct aaaccactat ctatatcttc atctatatag ttttcattta
     6541 aaatttcatc taaatcaatc tcgtaataaa cttcattatt tttttcatcc cctgagtcca
     6601 atcctaagaa ctcatagtta aaagtccaaa cattgtattt acttgacata ctttattcct
     6661 cctgtgaaaa acatattttc atctagttta ttggttccat aatcaacaga gttatatatt
     6721 ttttcaatta tgtcttttct tctttctttt gtactattat ttaatgtcaa ttgtttaaat
     6781 acatatgtat ttgttattct ctcgaataat atttctttta tactatcatc atcaatattt
     6841 ctatagtttt tccacaataa ctcaaataac ttaaacattt ccctactagt ttcatctaca
     6901 taataaggat acatattttc tactaaatgg tttaatcttg atatggcttg ttttaaaaat
     6961 acatcaagac ttgcactttc actttctttt aacattctat aattgtattt cttcttatct
     7021 tctcctgaaa ttacaaagat gatattgtct aaactttttt caacttcatt taaataactg
     7081 cttatattat atgataagtc agacaataga tttttaatct gcttaaacac ttgtacatca
     7141 agtttagtat tatacttttc aaacaaatct atttcttgat tttctaatat tcttggcata
     7201 tatgctgaca ataaatctaa attgtttctt tgttcttgta tttgcttata aagtgaattt
     7261 ataacttcta ttcttatttc atcatttaac tctttttcta aaatcttatc aaattcagat
     7321 ttcatatagt aaatatttcc aaaaaaatta aatattatca tatcattcat agctttagac
     7381 caattcttat atatcacttc aatagaattt tctttaagta gaggttgctt tgcatatata
     7441 aatacatctt gcataagttt ttgaaccata tttttactta agtttaaaag tactttttga
     7501 ttattaaatt ttatttccgt ttctacaaaa ttagaatttt cgttttcaag ttctttatta
     7561 attacaatgt cttttatatc actatttttt atagatatga tttctttttg aaaagacttt
     7621 agaatagtca agctatcgaa atcttcttga ataaaaaatg gagcatgttt taatgtatat
     7681 cccactattt cattttctat atcttttaag tcatctattt gcataatttc tctattttta
     7741 tcatttatat gtaggttaaa tacattttta ttatcatata atattatatc ttctcctaaa
     7801 tttaacttag aacaattatc tttcgctgac tttacaatag tcttcttaga tttcttattt
     7861 ataaaaatta attggtcttg atattttact aaaagcatgt ttgtgttttt ataatcaaat
     7921 ccatctatat ttcctgatat cttgcagaat actatatcac aatctttagg aagatttcct
     7981 attttttggt tatctttaac tctctcattt aaatcagatg gaactacact atctatattg
     8041 ttattaaata tagatacaga ccttactata atcccctctc tttccatatg aaagtatccc
     8101 ttcaatgaca ctcttttatc ttctaaaatg tatctttcta tatcatcaaa atttatttca
     8161 aatctatttt tcccactgat aaatataaac ttctcattat ctttattaat tccacccata
     8221 taaaacttat tgtcatctaa acttaatata gtcatattat cctttagata gaatataaat
     8281 tctctttgac ttttgcagat taaatcatat tcatccaaag ttattttcat agtcaaattt
     8341 gctgtaacat ttttacttga attttcactt tttatgtaaa aaggcaagtt atttccatag
     8401 acaattattt gagaatcttc taaaactgcc tctaattttt ctttattgtc tgaaaaacta
     8461 tatatatatt ttacacctgt atcctcatta acttcatctg atttgctgat tattatattc
     8521 cctagatttg ttatatgaag ctttttatat tcttttgagt atctacttat gccactaagc
     8581 tcaaacccat cagaaagctc atatatgatt ttttctccta atttcaacat accttcacca
     8641 cctaaacaga attataagta ttttataaag gtgaataaaa tgtctcaaaa caaataatat
     8701 ttttttaata ctttttattc tgagacatag tttctccttt tttataatgc tctaagcacc
     8761 ttcaactatt tcttcataaa gtttatctaa atgtttaaca tattcacgat atccagattt
     8821 gttctttaac tcacttctta aatcttcata tttttgttta agttcttcag cactcaaaga
     8881 aggagctgat tcttttaatt cttctaactt ataatcatag tacttatcat acatagctat
     8941 atatgtatca atagatttct tagctagttc attacatttt tcttgtgctt ctccatattg
     9001 agaataatca cctattttta ttttatagta tccattttct tgaattattg ttgaagaaat
     9061 acctttagcc tcatattctt ttgctgtttc ttttgctgtt ttttcaactt taagtgaacc
     9121 tgcataaaca gtgtaatata cacctttacc atctttatat gcttccattt cttttgcttt
     9181 cttttcagct tgtgcttttt cttgagcctc tttttcagct tttgcctttt cttctgcttc
     9241 ttttttctct ttttcagctt gtgctttttt ctcttcctca gttaatttat tgttagcttg
     9301 ttgttgagtt gatgactttg gaatattctc tttatcattt ttcttattgt tgcctactgt
     9361 tataaaatat cctacaccaa caataaccac tataactact gctataatag tttcttttaa
     9421 tctactcttc ttagaaacaa cctcttcatc atctaaagaa tcttctttat ctgatacata
     9481 atttttcatt tctttcttgt tttctctata tttgtcctta gaagaatttt tagatttttt
     9541 attactattt tctttctttt taataggtat tactactgat ttttcatttt ctaatttttc
     9601 ttcttttatt tcttttgctt ttttatcttc taagttttta ctttcttttt caagcttcga
     9661 ttcattattt gaagatactt tttcatctaa gttattattt attggttttt ctatttttac
     9721 actacgttct tcttctatat gttgacttgg ttcttcttgt tttatttctt ttaattcatt
     9781 ttgctctatt tcttctgatt ttgcttgatg attttcaact ggtttatcta aaattatatc
     9841 aggagaaata tcatttaaat tctcttcttc ttgttcaact tcttcattgt cttttttaat
     9901 aaatccaaat aatttagcct tctttttact ctttttattt tctggttcaa ttatatcttc
     9961 tttattgctg tcgtactctt tatctgtagt atctttatct tctacaacgt cacctatatc
    10021 ttgattatta ctatctactt cttctttagc cccttcttca gcatcatctt ctattgaaat
    10081 actacttcta aagcaaatat ctttatctgc attttttatc atgtaaatgt attcttcaat
    10141 atcgatatct ttatttcttc tagttctttt tgatgcaatt tcatgatttt ctaattcact
    10201 atagttatct tttattagat tctgtgcttt attccatgta tcttcactaa tttctgtttt
    10261 atttttaatt acatagtcca caaattcctt aaaattttca ggaccaaatt ctccaattat
    10321 agtcagtctc attgcacctt ctataaggtc tctatttctt ctctttaaaa attctaatac
    10381 aaccgttgca caagcaaaat ctttaataat ttctttgcca aaaccatatc cttcaacaga
    10441 atgaagttct tctaatccac ataacttctt atattcattg tgtattgttt gagatatact
    10501 ttcattattt aagctattta acttatctat aaacttcgat tctatatcat ttgattttga
    10561 ttcatgtttt acttttgaat ttttaatttc atttagtagc atgtcatttt ctgaattatc
    10621 tttacctaat tgcaaatcct caatttcagt tgcttttatt tctactttgt cttgttcgtt
    10681 attctcttcc acattattag gtaccacatc tttattgcta gctaatattt tgtttacaca
    10741 gaatataatt tctcttttta attctctttt cgcatctgct tgatttttaa gaaataaatc
    10801 tccacattct ttcttttcta aacttatttt tctaagagat atattaagga ggtctttata
    10861 taggtttaaa accttataat ccttttctaa acgtcttctt ctctctgata gttccatttt
    10921 atcataagct aaaaaactat ttaaatcatt ggcttttaga gagtctaatc cctctttcat
    10981 agccttattt acatactttc tacattttgc tgtagttaat tctaaatctt tttctaattc
    11041 atctatttca ggtatgtact tatctactat ccatttgcca ataagtggtt ctgtatttct
    11101 tgctaatgtc ttataaaaca atatattaaa ttttaaaagc tctgcatatt ttgcttcatt
    11161 aaattctact ttttcaaatt tcttttccaa aacaccatct agcttttcat ttattttaga
    11221 ctcttgtttt aaaaaacttg ctgtgacatt taaaacagtg tttcttatta tattattttc
    11281 actcaaaacc ttcactcctt aaatcaatta ttttattgta atatttacat tgtgcaaagt
    11341 tgctaatttg attatgatat ctaaataatt atttactaaa tttgctacat cttatatcat
    11401 atcatattat ttacatcatt ttactttttc tttttatatc tatcttatat tataccattt
    11461 ttaatatctg tactaactta tattacaata ttcctcaaaa ttttaaaagt agattagaca
    11521 aatttacaaa taaaaaaaca tcagtttaaa ctgatgtttt tgactatcga aataattaag
    11581 caagtctata agcggagttc tgtattagat aatcatcttt ctaggcctat tgtcaccaat
    11641 aggctcaagc gacctaccta ccggagagta cgagcagcac tctttattcc tatcttggtc
    11701 ttgctccagg tggggtttac atagccaact agtcgcctag ctgctggtga gctcttacct
    11761 cgcctttcca tccttaccac aatagtggcg gtttatttct gttgcactat ccttaggatc
    11821 gctcccacta ggcgttacct agcaccctgc tctatggagc tccgactttc ctcatgtaag
    11881 attacatgcg attatctggc ttacttaata tttattttac ttagttatgt taacacaaat
    11941 tcttttacat gtcaaagtaa tattatacta aaaatttaaa tttattcaaa aatagtgtaa
    12001 tatagatata taaactccat ttaactcgag gtgatatatt gaaaaaatta tatagaatag
    12061 taattaacat tatactagta ttagtaatac tttacagtgg atttaatata tactcaaaac
    12121 ttaccaaata caatcatgat actaaaacat cctcagaact tcaaaaaaaa gaatataaaa
    12181 aagaggattt atcaaagatt aacagtgatt ttaaattttg gctttctgtt gagaatacaa
    12241 atataaatta tccagttgta caatctaaag ataattctta ctatcttgac aaagattttt
    12301 ataaaaaaga ttctatttct ggtacactgt ttatggatta tagaaataaa tctattgatg
    12361 acaaaaatat cataatatat ggacataata tgaaaaataa aactatgttc aacaacctaa
    12421 ataagtttaa agatgctgac ttctttaaaa acaataataa aataaaaata accttgaatg
    12481 gaaaagaatt tttatatgat gttttttctg cttatattgt agaatctgat tatgattatc
    12541 taaaaacaaa ttttaacaat gaatctgatt atcaaaatta cataaatgat attacttcta
    12601 aatcattgta taaatctcct attaaggtaa atagcaatga taaaatagtt acactttcta
    12661 catgtaccta tgaatttgat gatgccagaa tggtgattca tggtagattg atttaaaact
    12721 cacagacaca gtttaaaaca ataattttaa actgtgtcat cttttatacc tataatttat
    12781 aaattaggcc actaaaagta tgtaatacaa aataacaaat acatcaaaca ataaaaaaca
    12841 ataaaaaaag actatgctaa aaacatagtc tttactggag ctggtgatag gaatcgaacc
    12901 tacaacctgc tgattacaag tcagctgctc taccgttgag ccacaccagc acacctattc
    12961 tattgtaaat tagtaaaact aatttttaaa agtta
//
LOCUS       DACTGW010000101        12919 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.101, whole genome shotgun sequence.
ACCESSION   DACTGW010000101 DACTGW010000000
VERSION     DACTGW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12919
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.101"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            19..1101
                     /locus_tag="I9573_002174"
     CDS             19..1101
                     /locus_tag="I9573_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAT4796791.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            1250..1411
                     /locus_tag="I9573_002175"
     CDS             1250..1411
                     /locus_tag="I9573_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HAT4796792.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            1752..3020
                     /locus_tag="I9573_002176"
     CDS             1752..3020
                     /locus_tag="I9573_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAT4796793.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            3215..4003
                     /locus_tag="I9573_002177"
     CDS             3215..4003
                     /locus_tag="I9573_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAT4796794.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            4003..4452
                     /locus_tag="I9573_002178"
     CDS             4003..4452
                     /locus_tag="I9573_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HAT4796795.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            4456..6720
                     /locus_tag="I9573_002179"
     CDS             4456..6720
                     /locus_tag="I9573_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAT4796796.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKNALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            6860..7171
                     /locus_tag="I9573_002180"
     CDS             6860..7171
                     /locus_tag="I9573_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796797.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            7457..7957
                     /locus_tag="I9573_002181"
     CDS             7457..7957
                     /locus_tag="I9573_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAT4796798.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIISGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            8014..9000
                     /locus_tag="I9573_002182"
     CDS             8014..9000
                     /locus_tag="I9573_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HAT4796799.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            9050..9772
                     /locus_tag="I9573_002183"
     CDS             9050..9772
                     /locus_tag="I9573_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HAT4796800.1"
                     /translation="MENFNKNAKIIGIGVEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            9801..11735
                     /locus_tag="I9573_002184"
     CDS             9801..11735
                     /locus_tag="I9573_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAT4796801.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFIEESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALDGGYFFPVRWIEYGKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            11728..>12919
                     /locus_tag="I9573_002185"
     CDS             11728..>12919
                     /locus_tag="I9573_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HAT4796802.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKIRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDECSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLGYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIE"
BASE COUNT         5179 a         1175 c         2281 g         4284 t
ORIGIN      
        1 aaatttagga gaaaataaat gagaaaatta tccaaaagag aaaaagattt tattcaatat
       61 ggatttttat tatttttgat atgtattact acttatttag tattaactac tttagatata
      121 aaactaatac ctaaaattat aaggctagta aataagactt atatatttct tggcatattg
      181 acaattatag tatatatgat aattgaatct attgtaactc atttgataat aaatgcagtt
      241 caaaaaacaa aagtaaaatt tattggtatt aaaatggcta caatgggatt ttactataac
      301 ttagttactc catttgcatc tgggagtcaa ccaatgcaaa tatatgcact tacaaaatat
      361 gatgtaagcc ttagtaaatc agtggctata gttactaata aaactgtagt atttcaaagt
      421 acagttactg tattttgtgg aattttaatt ttgctaaata agtcattact gatggagcaa
      481 gtacattctg tgaaagtctt gataactttt ggaatgatta tgaatgtaag tatgttgtta
      541 ggtggactat taatagtatt tagtccaaaa caagtaaaaa tgatagcaga agttttgatt
      601 aattttttat ctaaatttaa aacatttaaa tttttagatt caaaacgtga tagaataaat
      661 cattatattg atgattataa ttattctata aaaatattta ttaaaaatgt aagaatctta
      721 gttttaagta tattacttac tgtgttacaa ttaatcgcat atttcagtat agtttattgt
      781 gtgtataaag cctcaaattt aagaggagtt tcatatgtac atataatgac attacaggtc
      841 tttttatata tggctatatc accaattcca acaccaggaa atgttggagc taatgaggtt
      901 acatttttta ctatgttctc caatataatt ccaaaagaat tattggggta ctctgtactc
      961 ttatatagtg ggtttgtata ctattttata ttaatagttt caggtttatt tacatttgga
     1021 acgcattata cattaaattc ttggaaaaag gaagataaag taaattctaa tttagataag
     1081 gttattgaaa aattaaatta ataaaataat tagggaaatt ctatatataa atatggtgtt
     1141 gagtaagcta tattatattg agatatttcc cttttttgat taaaattata gaaatttgaa
     1201 ataataagta ataaacagaa aaagtgatat tgttttgaaa ggagaaacta tgaaacaaga
     1261 taaacaaaat gaaaaacaaa gtgagatttc taagataaaa caaagcaatg ataataaact
     1321 tgaaatagct caagaattac atgtaagtaa aaatataggg aatatgcttg gatatagaat
     1381 agataagaaa aaaatgaata gaccaagtta atgaaaatgt tgaaaatggt acattttaaa
     1441 aaatatatat aatgaaatgt tttattgtta aatttaaact tattttaatg gaggaaaaag
     1501 tcgaaaaata tctaaaaaga tatggatata aaaaatatta atatatatat actataaact
     1561 tgacaaaatc aaccttttaa taactagcaa aaaatagcaa aattatataa gcgttattta
     1621 attatcaaaa atgaaagcta aattgcatgt cctaaaaaaa atgattgata acatttattt
     1681 gtgtgctata attatatagt aaaacaataa gtatattgca taatgtatgc aaatcgggag
     1741 gagagacgga catggaagca tctatacaca caattgccag caataattta ttgataattt
     1801 ttgctattgt aagtataact ggaataattt gtagtaagct tagtgaaata ataaaggttc
     1861 cagatgtagt gttatattta cttgtaggta ttttaatagg accatcattt ttaaaattta
     1921 tagatattcg tggatttcaa atagaaaatc aactaatact tacatttggt tctgcattta
     1981 ttttatattt aggtggaaaa gaaataagtt taaaagtatt gagaaatgtg aagatatctg
     2041 tattcttatt agctacttta ggagttgtga tatctgcttt tataatgcaa caagtaatag
     2101 gatttacttt tgggataagt gcaatgactg ctttactagc aggaactata atagcatcaa
     2161 cagacccagc cactcttgta cctatattta atcaggtaaa gataaaggat agggtaaaac
     2221 aaactgtaat aagtgaatca gcttttaatg atgcaacagg agctatacta acatcagcag
     2281 tgttggctgt aattttgtca aataaatttt cattaggtga aaatatttat gaactgggga
     2341 caatggttat tgtcggagtt ttagttggtt taattacagg aatactgctt ttgaaattag
     2401 ttaatgataa accttatgga atatttaaag attttgcacc aataatatct gttatttcag
     2461 ttgtagttgc atatgaatta tctacaaaat taggtggaag tggatatatg tcttgcttta
     2521 ttgtaggtat aataactggt aacaagaaaa actttaaaat ttggttaagt caaaaatctt
     2581 atgatgcaga tttttatgtt gctgaaactt taggtactat ttgtcgtatg gcaatattta
     2641 taatattggg tagccaagtt aatttggctc agttaagtaa atattttatg ccatctgtaa
     2701 ttacagtatt agtattaatg tttatagcaa gaccactttg cgttttagtt tgtacacttg
     2761 tagacaggga agcaaaatgg agtaaaagtg aaatattatt tatgatgtgg gtaagggaaa
     2821 caggcgtaat accagcagca ctttgtggta ttatttcagc aatgaaagtt cctggatacg
     2881 aagtgatttc atctgtagta tttatgacta tattaattac attagtaata caaggaagta
     2941 ctacaaaatt agtagctaaa aaacttggat tattagaaga agaagtgata aatataagtg
     3001 aaaaagtatc aactttttaa agttttgctt taaaatagta cctgttaatg gctattttaa
     3061 tattgtctaa tgatgattat tgaaatagtc atttttttat gtataagtaa tttacagaat
     3121 ttacatatta gacatattta ttatcaaaat ttgttgaagc tatgatataa ttagttgtat
     3181 ttaaaataag aatagcaaat aagggaggaa atttatgttt acggttatgg atattgtaca
     3241 gccagataca gtagaagaag cgtactcaat tttaaataaa agaaaaacta accaagtaat
     3301 tggtggaagt gcttttttaa gaatggggaa aaaaagaata ggaacaggaa tagaattatc
     3361 taaattaaat ttagattata taaaagagta tgaagattac gttgagattg gttctatgac
     3421 tacttttaga acattagaaa caagttccat aataaaaaat aattttggaa gaataataga
     3481 agattcagtt aaaaatataa taggagttca gtttagaaat gtagtaacag ttggtgcaac
     3541 agtattttct aaatatggtt tttctgactt gatagtagca ttactttcat tagatacgga
     3601 agtagaatta tataatattg ggagaattag tcttgaagag tttctaaata gagattatga
     3661 gaaagattta cttattaaga tatacataaa gaagacaaat aagaatgctt cttataaatc
     3721 acttagaaat gcaaagagtg attatccaat tctaaatgta tctgtatcaa aacatatgga
     3781 acaatttaag ttatgtgtag gagcaagacc gcagaaagct acgattgcaa aacaagctag
     3841 tgaatttcta tctaacaatg aaatcaatga aattaatata gataaagcag tagaaatagc
     3901 atcagaagaa ctaacttttg gttctaacat gagagcatct agggagtata gaaaagctat
     3961 gagtaaagta cttttaaaaa gagctataat ggaggttatt taatgttagt aaagattaat
     4021 ataaatggga agaaaaaaag tgttgatata gaaccagaag aatatctggt tgatacttta
     4081 agaaaagttg gtaatttaag tgtgaaaaga ggttgtgaca caggctgttg tggtttatgt
     4141 acagttttaa ttgacaagaa acctacatta tcctgtgcaa cactgacatt gagagcttta
     4201 gataaagaaa taactacaat tgaaggttta gaaaatgaag ttaaagaatt tagtgaagtt
     4261 ttggtaaaag aaggagctga gcaatgtggt ttctgttctc ctggctttat acttactgta
     4321 cttgcaatga aggatgagct tgaaaatcct acagatgatg aaataaagca ttatcttaca
     4381 ggaaatctat gtagatgtac agggtatatg ggacagttaa gagctatcaa aacttatctg
     4441 gaggtaaaat agaacatgaa tatagttgga aaaggaatca agaaaataga tggaatgagt
     4501 atcacaacag gaaagcctgt atatacagat gatttatcag caaaaaatgc tttggtggta
     4561 aagatactta gaagtcctca cgcttatgca aaaataaaag atatagatac atcaaaggca
     4621 gaaatggttg atggtgttga atgtgtttta acatataaag atgtaccaaa atctcgattt
     4681 acacttgctg gtcaaacata tccagagcca tctccatatg atagattaat tcttgaagat
     4741 gttgtaagat atgtaggtga cgaagtagct atagtggcag ctatagatga aaaaactgca
     4801 attaaagcaa tgaatcttat caaagtaaag tatgaagtac ttacacctgt tttggatttt
     4861 gaagaggcaa taggaaataa aactatggta catgatgaag atagacattg taactttgat
     4921 ataggtatgc aaagagaaaa aaatatagta tcccatcatg attatacaaa aggtgatgta
     4981 gaaaaagtat taagtaattg tgatgtagtg attgaagaaa cttattatac tcaagctcaa
     5041 gcacatgcaa tgatggagac gtatagggca tacaattatt tagaccatat gggaagattg
     5101 gttgtagtaa gttcaactca aataccattt catgtaaaaa gacatttatc tcgtgcacta
     5161 gaaataccag ctagcaaaat tagggttata aaacctagaa ttggtggcgg atttggtggt
     5221 aaacaaactg cgtgtgttga gattttttct gcaattgtta ctttaaaaac agggaaacca
     5281 gctaaaataa tttatgatag aaaagaaact tttaattgtt caacaagtag acatgcaatg
     5341 agattaaaag ttagactagg tgctacaaat gatggcataa taaaggcaat agatataaaa
     5401 gcactttctg atacaggagc atatggagag catgcttcta caactattgg tttggttgga
     5461 gaaaaaactt taccaatgta taataaagtt gaagcaacaa gatttattgc agatgtggta
     5521 tatactaata agatgccagc aggagcactt agagggtatg gagcatcaca aggatgtttt
     5581 gctgttgaat caactataaa tatgcttgct aataaattaa aaatggaccc aacagattta
     5641 aggttaaaaa atgtggtaac agaaggtgaa actacatttg catatggtaa aactttaaat
     5701 agtgtcgatt taataaaatg tataaataaa ggcaaagaac ttattggatg gaatgaaaaa
     5761 tatccttgta aacatcttga taatggaaaa attagaagtg tgggtatggc aattactatg
     5821 caaggttctg gtatacaagg tcttgataag tcttcagctg aaataaaatt gaatgactgt
     5881 ggagattata ctcttatgat aggttctact gatatgggta caggaagtga tacgatttta
     5941 gctcaaatgg ctgctgaggt tcttgataca agcattgaaa atataacagt tatatcagct
     6001 gatactgact tagttccata tgacccaggt tcatatgctt caagtactac ttatgtaaca
     6061 ggtatggcag tagttaaggc agctcaagac ttaagagaaa aaataatttc tgctggagca
     6121 actaaactag gtgttgagaa ggatgaagta gaatttgatg gtataaaatt aactagtaaa
     6181 aaaggtagca ctttattatt cgatatagca gttgattcta caattggtcc agatactaat
     6241 caattaatag gtcttgcttc tcatggtagc ccagtatctc ctccaccttt tatagcaggt
     6301 tttgcagaaa ctgaaattga cacagaaact ggaaaagttg atttagtaaa ttatgtagct
     6361 gtagtagatt gtggaacggt tataaataaa aatctagcta agatacaagt tgaaggtgga
     6421 attgttcaag ggattggaat ggcattattt gaagaagtta gatacacaga aaaaggaagc
     6481 atggacacaa atacatttat gcaatacaag attccagcta gatgtgatat tggaaatgtt
     6541 aaagtagatt ttgttgaaac ttatgaacca actggaccat ttggtgctaa atctgttgga
     6601 gaagttgtta taaatacacc tttaccagca atacaagaag ctgtgtttaa tggaattggt
     6661 gtaagaataa atacactccc tattacacct gaaaaagttt ttatggaaat gaataaataa
     6721 ttatttatga aaatttagac tagttttaca tgttaaacaa ccagttatgt catacctgtt
     6781 gaaagatatt taataagtag ttattataat attatgaatt ttacttataa aatacaaaat
     6841 taaataaggg gatgatgttt tggctggtaa tctaaataat atgagagcag taaataattt
     6901 tagaggagat aagaacattt tagaatgttt agtcagcttt gagggtcgtt caataagtca
     6961 gagaaaagta agggtatttt ttaaagaaaa acaaaatcaa atagaaattg attttgcaga
     7021 agaggaaatt tctaaattgg ttgaaaatgt tgttttaaat acatcatatc aagaaatgtt
     7081 atatgatgaa atagagaaac aactggaaat tgattgtata ggtacttgga tgatattatc
     7141 taaattaaaa gatggtagta gagttcactg attttaaaat aaattattag agcctaattt
     7201 taaggctctt ttttgctgtt tttaattaac aatagaaaat ttatctttag tttgtatata
     7261 attttaatat catatattct atatatataa aattgaattt gtatgtatta tattgtataa
     7321 tataaaatga atttggaaaa tagttcgcta aaataatttg tttttataaa attactgttt
     7381 aagtttatag attttgtata aaataaagtt atgttattaa tttttttaga aataaaactt
     7441 ttactaggag acagatatga gaaaaaatag tattaatata gcactaactg cagcaattac
     7501 agcaatctat gcagttttaa cattatcatt gggatttata agttatggac caatacaatt
     7561 tagggtagca gagattatga tgcttttggc atttttagat aaaggatata tagtaggtct
     7621 tacattaggt tgttttttag caaatgtaat aggaccatat ggtgtacctg atataatttc
     7681 tggaacatta gccacattta taagtgcaag tatgatttat attactagaa agttaggtaa
     7741 acaaaataaa agtactttag ttatagcttc tttatggcca actgttataa attcgattgt
     7801 ggttggtcta atgttaaatg tattctttgg gcttccatta attctatcta tgatacaggt
     7861 aggttttgga gaatttgtag tagtaacaat tgtaggagta ccatttttct gctttttaat
     7921 gaaaaaatat aaaaatgtca ttaatattaa attttaaata gtgtatagta atttaattaa
     7981 ttgagagtat tattagtgaa aagaggtgtt tatttgtcga aggtaaaaag attgactgat
     8041 gtttggatat atattaatga taagcaaaag tttacaataa aagagttgtc agaggaattt
     8101 aatctttcag ctaaaacaat acaacgatat ttaaaagaat taaataaaat gggacttcca
     8161 attcaagcag aacaaggtag aaatggaggt tatagggtgc taaataatag ctatatacct
     8221 cctgttatat ttacagaaaa agaagttatg attatactat ttgcacttaa gtcacttcag
     8281 atttataatt ataagttaat ggaaatagaa attaattcta taatgagaaa agtaagttat
     8341 gaacgtaaaa gcagtataaa agtaggtata gaaaatatta aaagatacat tggctttgta
     8401 gctaatgata ctaatgatgg agattttaag agtagtaatg taactgagtt ttttagagct
     8461 tcttcagaat ctctaatttt aaatattaag tataagtgtg gtaatcagat tttggaaaaa
     8521 aacgtatgcc ctataggtat atatttaaat aagggagcat ggttttcacc agcatatgat
     8581 tgtaaaagtg ataaaataat attagttcat atagataata taattgatat aaaaagaaca
     8641 ggagaaagta aaaaaataca tataaacttg gaagaatggt tacatgaagt atataacaat
     8701 ttatatggaa atagttcaac tactaaaaaa tttggtgaaa ttatttatct aaatgtatta
     8761 ttaactaaag aaggtgtttc taaaataaag gacagttctt ggaatttaga tatggagcta
     8821 aatgaagatg gtagtggaat aattagtaca ttcataaagg ttgaagatat agattgttat
     8881 gcttcgattt tattttttat tggaaataat gctaaggtta tagagccaaa atttttgaat
     8941 aagctacttt atgataaggc caatgagtta agatattttt atgaagaaaa tatggtataa
     9001 tatttagaaa tgttgaaatt aaataattat taaggagata tacgttataa tggaaaattt
     9061 taataagaat gctaaaataa ttggtattgg agttgaaggt attaatataa taaatgaagt
     9121 agaagaaaaa ataaaagcaa atatggatat agaaaaaata aacataaatc aagagattga
     9181 aaaagaatat gttagaagtc ttttagatgg tgtagatata ttatttttga tatattcatc
     9241 tgaagataaa catattagag atattataaa agctgtttca tatatgtcaa acgaaagaag
     9301 agttctatct ataggaatgg attgttcaga gaaagaaaat aaagaagatt tagagcttgg
     9361 gagagagttt aaaatcaata atgatagtat atttaaattt gttgatttaa tgaatatcat
     9421 ggtagagtct atttctgatt cttgcatgat aaatatagat attactgatt taaaagaagc
     9481 tattgtagga gataaaggaa ttaagtattc ttttgaagag tttgaagata caaagtcata
     9541 tagcgagata gctgacattt tatttgacag gatggaatat attggtgagg aatttataag
     9601 taagaaagga attgtatttg ttgaaggaag tccagaattt tctataatgg aattgaatga
     9661 tttaataagt aatattcaga gcaaggtaga agaaagctat gaagtaatat tttctttata
     9721 tataaaggaa aatttaaatg gaaatattag agtaggctta ttgtataatt agtatatttt
     9781 agatattaag gagagtgtat atgtctgaaa atataagagg tacagagtat tggttaatgg
     9841 aagaattttt ttatcaggaa acagacacac cacagatttt tatagaagaa agcataattg
     9901 cacttggatt tgatataaaa ataaatttaa aaaaagtatt cagtatgggg aaaaaatcag
     9961 aaaagtatat taatgagaat tgtaatgata aagattttat aaaaaagttt atgtcgatta
    10021 aaaaaggaga ttatttttta tataaagcat atcaaaatat agaagaaaaa gttaaatgta
    10081 atgtgtctat tggcatagtt gaagaagatt ttgaaaatgg atatgaatta agtcatattt
    10141 tagggcatac actaccagta aaatggatgc ataattttga tacagtttca aattgtaaag
    10201 tatatacaag tgaattttat aaattaaata ataatgatat tagttcatat ttagaaatta
    10261 ttaagtcaca agataagaaa ttattgagaa aaagaataga agaaagtgaa aataataaat
    10321 ggtatttaaa aaactattat tataaaaatg tagataagtg tgtagatgat gaagaattat
    10381 ttattcagcc cattaatcta gaacaattag aaagcctaaa aaatataaag acagatgatt
    10441 atattttatt gtataatagc gagaaagtaa tttgtgaaaa tgaccataca atgaagagtc
    10501 tatttggcgt atgtcaagtt atggaagggt tcgataaaga gaaagtaagt gctttggatg
    10561 gtggatattt ttttcctgtt agatggatag agtatggaaa ggagaatata gacggaaatg
    10621 catgggttat atttgaaaat aatagtatag ttgaaaagta tgtaaaaaga tttactaata
    10681 aaatgccatt aaatactata ctttatggac caccaggaac tggtaaaaca tacaatacaa
    10741 aacagatggt ttacaatcta gtaagtgaaa ccagtctaag aaacaaatac tatacaggtt
    10801 acttagatta taaaaacaat gtagaatttt gtacatttca tcaatcatat ggatatgagg
    10861 aatttataga gggtcttaag tctgatggtg aaggcaattt taagccagaa gatgggatat
    10921 taaaggatat atctatagaa gcatgttata atgcattgaa atttgaaaaa aagatgagct
    10981 ttgagttgga aaaagatagc ttgactttag aaactataaa agacaggaaa aagaaattag
    11041 tattagaaaa tatagaatat agtgagagtt ttaattttga agaatcagaa aactatgttt
    11101 taataataga tgaaattaat agagggaatg tatctaaaat atttggtgag ctaataacat
    11161 tattggaaga tgataaaaga cttactaaat caaatcaaac tattgtcaaa ctaccatatt
    11221 caaaggaaaa attttctcta cctccaaatt tgtatatagt tggaactatg aacacatcag
    11281 ataaatcaat agcactttta gatatagctt taagaagaag gtttaatttt gaagaaatta
    11341 tgccagatta tgatttattg tctattgtag atggtataga tattagaaaa atgctatata
    11401 caatcaataa aaggatagag tttttatatg atagagacca tgttattgga caagcttatc
    11461 tacttgctgt agaaaatatt aatgatatag tagtagtatt tagaagtaaa ataataccac
    11521 ttcttcaaga gtatttttat tatgattcag aaaaagtagg tttgatttta ggaggaattg
    11581 gaagaaatga aaaagataaa tacattgttt ataaagaaaa acttatagct tctgagttat
    11641 ttaaaaataa taatgcctca ataatggaga gtaaagttaa ctattgtata aaacaaaaca
    11701 tatctagtga ggagctaaaa aatatatatg agtaaaaata tatatgttaa ggaaacctat
    11761 gaatggataa gggtaggtaa tggtgaaaat gagcttacag aaattgaata tgaaaaattg
    11821 cttaaatatc tagagaacaa taatgatgta ttaaaaagta atataataga tattaaatac
    11881 aaaaaaatta gatttataaa ctatgtaggc atcatatgtt ttgaaaatgt aatacttgaa
    11941 atacttccta aattgtcatt aagtgataat cttgtaaaag atagagagat actattacag
    12001 atgctttcta tatgtaataa aattccaatt acaatgaatg aaaaaataag attgagtttg
    12061 aaaaattata atctcttaaa tttttttgtt atgtatttca ttgagagtat gcaaacacag
    12121 atgaaaaaag gtatttattt tgaatatata aataaaatag agaacttaaa tgttatgagg
    12181 ggtaaaatat tattaagtac atatgcaaaa gaaaaaggca tttctccaat gaaaataaga
    12241 tgtgaatatg atgagtgtag tgaaaataat ttcttgaatc aagttcttaa aaaagcatgt
    12301 atatctattt tatgtagaat aaatgacaat tcaattcaag gcaagattaa aaaaatttta
    12361 agctattttc aaaatgtaga tttaatttat atagatagaa aaaaactgtt gggttataaa
    12421 ttttataaaa acaatgatag atttaaagat tgttatttac tagctagact tctactttta
    12481 aatctatcta tggataatag tcaaaataat caagaggcat tttctatatt atttgaaata
    12541 aatactttgt atgaagaata tataggaatt ttaatcaaaa gtatatggga caattcattt
    12601 agagaaacat atatacaaga taagagtaaa tttcttttga aaaatgaaca aacaggtaag
    12661 aaaaacttta atttaagacc ggatatagtt ttatatgact taaaaaatga atatgaaatt
    12721 ataattgaca ctaaatggaa agctattgaa gtagattcaa atgtttttta taggtcaagt
    12781 gatatatacc agatgtatgc atatataaca gcatatgaaa atgcaaaaag atgtatatta
    12841 ttatatcctt gtattcaaaa agataagaat tatagttcct ggaaattatc agaatcattt
    12901 aagggtaagt ttatagaag
//
LOCUS       DACTGW010000102        12765 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.102, whole genome shotgun sequence.
ACCESSION   DACTGW010000102 DACTGW010000000
VERSION     DACTGW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.102"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(167..499)
                     /locus_tag="I9573_002186"
     CDS             complement(167..499)
                     /locus_tag="I9573_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAT4796803.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(743..1384)
                     /locus_tag="I9573_002187"
     CDS             complement(743..1384)
                     /locus_tag="I9573_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAT4796804.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVILQI
                     LMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGIILPWTLDDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(1365..1658)
                     /locus_tag="I9573_002188"
     CDS             complement(1365..1658)
                     /locus_tag="I9573_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAT4796805.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            2052..2111
                     /locus_tag="I9573_002189"
     CDS             2052..2111
                     /locus_tag="I9573_002189"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796806.1"
                     /translation="MIEYIYYYNNKRIKKINLA"
     gene            complement(2627..3295)
                     /locus_tag="I9573_002190"
     CDS             complement(2627..3295)
                     /locus_tag="I9573_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAT4796807.1"
                     /translation="MPVLMAIGVPVHLAIGTNKFAASAGCISSAYRYAKSGKINNDLL
                     KKLVPFTIIGSVLGVRCVLSISEEILNVLVVVMILIVAIYTFISKNLGQEDNFEAVNK
                     KNLRLGMLMALIMGFYDGFFGPGTGTFLTFGFIKIYGYDFLHASANTKILNLTSNITS
                     LLLFMINGQVDYKIAIVFALVMIMGAYVGAKVAIKKGSKMIKPIFLVMALFMVVKLVY
                     QTLV"
     gene            complement(3240..3380)
                     /locus_tag="I9573_002191"
     CDS             complement(3240..3380)
                     /locus_tag="I9573_002191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796808.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGTYKHACSYGNWSASTSCHW
                     YK"
     gene            complement(3439..5094)
                     /locus_tag="I9573_002192"
     CDS             complement(3439..5094)
                     /locus_tag="I9573_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4796809.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQMIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKGI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVDSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(5323..5691)
                     /locus_tag="I9573_002193"
     CDS             complement(5323..5691)
                     /locus_tag="I9573_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT4796810.1"
                     /translation="MKYSITDVSKITKISSSAIRFYEEKGLLLPINRKPSGVRYFSEE
                     DVERLIFIKELKNTGLSIKDIHECFHFCDMGDGTIDKRIQLFQQHKQNIKNKIHELES
                     CMDKIDEKIYYLKEKKMCKK"
     gene            5840..6634
                     /locus_tag="I9573_002194"
     CDS             5840..6634
                     /locus_tag="I9573_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HAT4796811.1"
                     /translation="MEKYATMIKEKLNEGKVLCGVAVSLSDSVVSELIGISGYDFIWI
                     EGEHSALDRKDIQQHMIAAHAGGAASLVRLVNSDPDLVKPIMDMGTDIICFPFINTVE
                     DAQKAVAACTYPPNGIRGYNPQRASYYGNMNYFDYVKNANNETLKMMLIEQKAGYDNL
                     EEIVKVEGIDIIALGPGDLSLDMGFSGYMNRIEIKDMISHAAEICKKYKMPFIIFPTI
                     EKKSIEEWVNIGANMLCFAQDTTFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(6787..7215)
                     /locus_tag="I9573_002195"
     CDS             complement(6787..7215)
                     /locus_tag="I9573_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAT4796812.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(7691..8629)
                     /locus_tag="I9573_002196"
     CDS             complement(7691..8629)
                     /locus_tag="I9573_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HAT4796813.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
     gene            complement(8973..10628)
                     /gene="ilvD"
                     /locus_tag="I9573_002197"
     CDS             complement(8973..10628)
                     /gene="ilvD"
                     /locus_tag="I9573_002197"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAT4796814.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIEFNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            complement(10952..11251)
                     /locus_tag="I9573_002198"
     CDS             complement(10952..11251)
                     /locus_tag="I9573_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796815.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            11511..12191
                     /locus_tag="I9573_002199"
     CDS             11511..12191
                     /locus_tag="I9573_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAT4796816.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            complement(12301..12558)
                     /locus_tag="I9573_002200"
     CDS             complement(12301..12558)
                     /locus_tag="I9573_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796817.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
BASE COUNT         4367 a         2056 c         1282 g         5060 t
ORIGIN      
        1 acaatctatg atgtaaataa tttaaaacta ctatattatt taaaaattta tatttatata
       61 tgcaattcta aaaacttaat aagaagtgaa cttagaaaat aaagcttaat ttcaaagact
      121 taaatagaat attattatta gtctaaataa actacgtgta tcaaaattat atattaaatt
      181 cttctaaaat ttttattgca aatcttgcgt ttggatatgt aatagacccg ccaccaatta
      241 cacctatttt tatagcttct ataatttcat ctttacttgc tccagaatta atacaaccct
      301 caatatgata acaaatacac tcatcacatc ggcttaaaat tgaaatagct agtcccatta
      361 attccttatt ttttttactt atagcccctt ctgaataagt cttatcatca agacttccaa
      421 atactctaaa aaactcatct gtttcactta attttttatt gtaaaatccc ctatattctt
      481 tcatttctct agtattcatc ttctacctcc tatgtttttc ttataagagt atcacactgt
      541 acttatattt tcaatattaa atacaaattt tcattttatg ttcattagcc taatttatat
      601 tattttttga aatctcaata agttatcata taagtaagat tttttcagaa ctaaaataga
      661 aatcttagta atttctttat tatctatctc agttctgaaa tatattattc ttatccaaac
      721 attattatag tgattaatta tattagttat ttttacctga aattttatac atcaaataag
      781 aatatataaa tggtagtatt acaattacaa agattacaaa gatattataa aaaggaatat
      841 taataaatgc attaataatt aataatattc caccaataag ccaaatccat ccagctaaat
      901 ggtgtgtctt attccaatta tcttcatcat caagagtcca tggcaaaatt attcctactg
      961 agtaattttg tttacattta ggaagatagt ttccaaataa aataactatt attcctaaaa
     1021 ttaatcctgt ataaaaatta gactccaaac taactcctgt tgaatataaa accatcagta
     1081 tttgtagaat tactgaaagt atagcaactc cccattttga taaattttgt ataacatttg
     1141 aaaaattagc ttttttaggc tcattctcaa gtctaaacca tgtatacaaa tggatacata
     1201 gcattaacaa tggtacacca aaagctgcta taacctttgg agcataatta tctggatttc
     1261 cataattatc aaaatgaatt gcaacttcat ttggtaatct attataaaaa tatattgata
     1321 caataaatgg tataaaacat attaaagtag ataaaataaa ttgtttattt atcgcttttt
     1381 tcattatctt tttctccctt ctccataaat tgtgaaaacc aaagcataac ctcttcgaaa
     1441 actgaaatat ttatttcgta gaaaataaag ttcttatact ttgtttcaaa tatgagtcct
     1501 gcctttttta gttgagatag atgataagaa attgtagcat tcgtcatatt aaatttttca
     1561 ctaatttctc ctgctgacat tttattctcc ctcaacataa ccaatatttc tcttcttata
     1621 gggtctgaaa gagctttgaa tgtttctgga aaccccaaca ccatcacctc gctatttaga
     1681 atttcatcta aatagaatat aacataacat ccattttatt tcaataacta tttagaaaaa
     1741 tttctaaata gttatttcca aatatttatt gaaaattttc atattaagat tctcaattta
     1801 cttaagtcgc ttttatataa tcttatttta tctaattttt ttaatttaac aaaagtgtat
     1861 tgcaccacaa ttgttaaagc aaaaacctaa tgactgatat acttatatat attacatata
     1921 cttttaattt caaattaaaa gtagctaaat ccaatttaaa aagtagttaa gtcctattta
     1981 aatagaaaag aatgaaataa gaaatatact gtataaaaat gcataaaaac ctctactaat
     2041 tagatacaac tataatagaa tatatttatt attataataa caaaagaatt aaaaaaatta
     2101 atttagcata accttgttaa atatagattt gaatatgaat ttaaagttat gtaaaattaa
     2161 aaatacagga atgaaaattc cattcctgta taaaatctaa cttttgagtc tcaatacaat
     2221 cttattttga gacaatcttt tttgttttat attttcttat atttaaaata taacgcatat
     2281 cttatgcaac atattcatat ttttctttat attctttcat cgcatcattc caagcgtctt
     2341 gtactgttac acctctttta ttagcaattt tttgtgccag ttcaattatc ttcatagctc
     2401 ttcttgatat gttcataaac attcctccta caaattacca tatatttcat atatataaat
     2461 caaggttata tttttatgat atataatcct tctattgtaa tattacaatt tatatgtgta
     2521 ttatatatga actatattag aagataatta gaatttttat aaagctatat gattagatta
     2581 aatataaaag ctttacaatt acttataaat ttaaaaagtc tataaactaa actaatgttt
     2641 gatataccaa ttttacgacc ataaataatg ccattactaa aaatattggt tttatcatct
     2701 ttgaaccttt ctttatagca actttagcac ctacataagc tcccattatc atcactaatg
     2761 caaacactat agcaatttta taatctactt gaccatttat catgaataat aacaatgaag
     2821 ttatattact tgttaagttt aatatttttg tatttgcaga agcatgtaaa aaatcatatc
     2881 catatatttt tataaatcca aaagtcaaaa aagttcctgt acctggacca aaaaaaccat
     2941 cataaaaacc cattataaga gccataagca tccctagtct taaatttttc ttattaacag
     3001 cttcaaaatt atcttcttga cctaaatttt tagaaataaa ggtgtaaata gccactatta
     3061 aaatcataac cacaaccaaa acatttaata tctcttcact tattgataat acacatctca
     3121 cacctagaac actaccaata atagtaaatg gtactaattt ttttaaaaga tcgttattaa
     3181 tttttccaga ctttgcatat ctataggcac tacttataca tcctgctgat gctgcaaatt
     3241 tatttgtacc aatggcaaga tgtactggca ctccaattgc cataagaaca ggcatgctta
     3301 taagtccctc ctcctcctgc tatagaatct acaaaagcag ctaaaaaccc ccctatacat
     3361 aaaaaaataa ctgaaaacat aaaattcctc catcattctc tttcaatttt tcttacaata
     3421 tatattatat aatcttattt aatacttatt atccagcata ttctttctat attcatttgg
     3481 agttatacta ttaaacttct taaatatttg tgaaaaataa ctactgttat taaaacctac
     3541 tttcttacaa atatcactaa tactcatgct tctatctaac agatattttt tagattcttc
     3601 tatcctaaga agatttatat actcagttat tgaaacttta tttctttcat taaaaataga
     3661 actcaaataa tttggactta taaaaaaaac atctgatata ctattcaaat ttatattttc
     3721 tttataatat ttttttatat attcgtttat attttcaatt agatttcctt tatcttttct
     3781 ccaaaatgct ctattcttat caatacactc tttggaaaat tcaaataata cattccataa
     3841 atcttcatca ctgtctacaa gtgatatttt tttattatat ttatttctta aataagatat
     3901 aagttttaaa tcctctaaat ctctaagcac tgttctagac aatacaaata tcatttcctg
     3961 aatagtatct ctaacatcat tatatcttaa tttgtttgta tctattagat tataaacacc
     4021 ttctaaattc atatttttaa tttctttttc aatataaagt tctaattcaa agggatattc
     4081 ttttgaagaa ttattgaaca tatctatcgg attaataata tactcttctt ctaataatct
     4141 actatttgct tttttaagtg cctcttcaag tcttatattt tcttttctac tctttaatat
     4201 ctcctcatct tttatactaa gttgattttc aaaaaaattc ctcttagcta tttcctgaat
     4261 aaattcagac atcattaata aattttcttt taatacatat aatctacttt taggatttaa
     4321 tggaaattca ttgtaaattt tgtttatgta ttttatatca ataccagaac tacttgataa
     4381 ttcagataaa tttttatcta tatcaataga cttatatact ataaaattac cacattctat
     4441 atatccaact atattttctt ctaatactat aggtatctct aaactggaaa caccatactc
     4501 acaatggtac actttttcaa agttatcatt atttatgctt ttaacaagtt ttttagtaca
     4561 cggttttata tttttacaaa tatcgcaaaa tttattttct ttcttagtct caaatattcc
     4621 tttatacttt aagtcatata cagatattct aagcttcaat atattagata tttgatttgc
     4681 aagattttca attatgttta cattaactaa ctcttgtata ttgttttcag aaaatactat
     4741 aggtatttgt ctcaaactcg aaaatttatg aggaacataa gtaattaata ttactaaatc
     4801 aattttacca gtgtttataa tttcatgttc tgaatatggt gggcaataaa ctatagatgt
     4861 ttcctttata ttgacctttt taccatcaat catttgtatt ccttctccct gaatcacata
     4921 taaaagctgt tcttcactta aatgaaaatg cttttcttgg cagtttcccg gaaaaaatct
     4981 tacaattcca gcactaagtc tattgaattg tgtttttgat ggttcgtgta tccacataac
     5041 ctctccccaa tcaaattttt gtacttcatt atttgactca ataaaatcca ccataaactc
     5101 acctcgtagt ttttatctaa atattttttt attcctttaa atatctcctt ataatataca
     5161 ttatcataat tttctttttt attgtataga taaatagtta tatagaacat tgtccaattc
     5221 ttcctaaaaa tatagtgtaa aaaatctttc ctcaaatgtt tccaattgaa tttactcttt
     5281 atattagaaa aatcaaaaaa ttatttcttt attctataag gattattttt tacacatctt
     5341 tttttctttt aagtaatata ttttctcatc tattttatcc atacaacttt ctagttcatg
     5401 tattttattt ttaatgtttt gcttatgttg ttgaaataat tggattcttt tatctattgt
     5461 accatctccc atatcacaaa aatgaaagca ttcatggata tctttaatag ataaaccagt
     5521 attttttaat tctttgataa atattagacg ctctacatct tcttcagaga aatagcgaac
     5581 accacttggt tttctattga taggaagtaa taaacctttt tcttcataaa atcgaatagc
     5641 agatgaagaa atttttgtta tttttgatac atcagttata gaatatttca taatctctac
     5701 ctccctaaat tttatatgaa taagaatatt ataaaaatac cttatacagt agtcaacaaa
     5761 aaagtattga cttcaagtta ggttgaagtt gtactctttg aagtgtaaaa gaaaacttac
     5821 aaaagaaagg atgatataaa tggaaaaata tgcaactatg attaaagaaa agctaaatga
     5881 aggaaaggtc ttatgtggag tagctgtttc actgtctgat tcagttgtaa gtgagttaat
     5941 cggtatatct ggatatgatt ttatttggat tgaaggagaa catagtgctt tagaccgtaa
     6001 agacattcag caacatatga tagctgccca tgctggtggt gctgcttctt tggtacgatt
     6061 agtaaacagt gaccctgacc ttgttaagcc cataatggat atgggaacag atattatttg
     6121 tttcccattt attaatacag tagaagatgc acaaaaagcc gtagcagctt gtacttatcc
     6181 tccaaacggt ataagaggat ataatcctca aagagcctct tattatggga atatgaatta
     6241 ttttgactat gtcaaaaacg caaacaatga aacattaaaa atgatgttaa ttgaacagaa
     6301 agcaggttat gataatcttg aagaaatagt aaaagtcgag ggtattgata ttatagcatt
     6361 aggccctggt gacttatctt tagacatggg tttttccgga tatatgaata gaattgaaat
     6421 aaaagatatg atttctcatg ctgctgaaat atgcaaaaaa tataagatgc cctttattat
     6481 ttttcctaca atagaaaaga aaagtattga agaatgggtt aatattggag caaatatgct
     6541 ttgttttgca caagatacta cctttatcac ttatgttatc aatcaattat tatcttttta
     6601 taatgagatt gtaccaattc aaaaacaaca ttaaattata aatctaattt tatattaaaa
     6661 gagactaata ttttatctat ctctattaag gatattttct tatgtatcat aatattagtc
     6721 tctcttatta ttatatactg ctatcatcat tagttgctat ttatactatt tttataatat
     6781 acaaatttat tcagctaacg ttttgcctaa ttctatacac tcatcttttg cttcttcatt
     6841 tgggtcttcc attgctgtaa ttccttctcc accaataatt gtagcaccag cattttgcat
     6901 acgttcttcc cagtctctca tccattcaca atttccccat ccatatgaac caaataatcc
     6961 aatctgttta cctgatacca tagattctaa ttctgaaaca aatggctcca tatacccctc
     7021 ttctagttgc tctgctccca tagatgggca acctaatgca aatttatctt cttcttttaa
     7081 atcacttggt ttcagtaagc tcacatctaa aacttctggt gtcttacctt ttaacttcac
     7141 accttcagcc acaaaatttg ccattttttc agtatttcct gttccactcc aataaacaat
     7201 atatatttta ctcattttta cctcctgtag tattttttaa ttaatcaata gttagttata
     7261 gctaactaac atctaaaaaa caaaagattt ctttatctga aatctcttta tttttctaaa
     7321 tatagaatat cacaattgat tttgaaattc aatatttatt gataatattt atcatttata
     7381 ttttatattc attttataat atcaaaacac tgtatcgtat ttctaatctt tcatagaaaa
     7441 atttctaact tatagtactt ttgcatctct tataaatctt ttatatgtat aagtacatag
     7501 aaaaacacac cttttccaga tgtgctatct ataatatcgt atatttattt ttcaattcta
     7561 actagctaat atgtttataa attttatcac ttaatacctt tatacattga tgtaaaatct
     7621 tacacctaag taatttttat tatttgtctc gactctatat aatatagcta ttttatcatc
     7681 aaatcaaaaa ttatcttttg ctattattct tgtatttata aagatttaaa tataagtatc
     7741 catttccttg ttccgttcta ggtattccaa gcgctctcgt acattttatt aattgtgcat
     7801 acagttcagc ttcatctaaa tctctttcaa actcctctct tgcccattct ttaattagtg
     7861 ctaatgagcc tgatacgtat ggtgcactca tactagtacc actcataata gcaagtttat
     7921 tatccatata agtcgatata atatttcttc ctggagccac caaatctatt gtagtatttg
     7981 aattactaaa cttttcaacc aaatagtttt catttattgc acctacttct attacctcag
     8041 cataactggc tggataacta tactcacttg tactagaatc accatctcca ttattacctg
     8101 ctgcacacac tacagaaata ttatttttta ctgcttgcat gacagcattt tttaaattct
     8161 tatcatcttt gtttccccca agagacatag atataatatc aaccttgtta tttacagcaa
     8221 agttaatagc attaattata ctttgatatg ttccggtacc atctttattt aatgcttttg
     8281 ctattaataa tttacaatct ggagctactc ccataacttc attattatag ttagatgcag
     8341 ctataatacc cgccacatga gttccatggc cattaaaatc ctcatatata tttttatttc
     8401 cattactgtc atcactaaaa tttgcaccac caattatttt tccttttaaa agaggatgag
     8461 atatatcaca acctgtatct attataccaa ctacaatatt tttaccagta taaccttcat
     8521 cccacattcc cctagcattc atttgtttta ttccatatgg gaatttattt tttgcacctc
     8581 ttagattttc atttatttca tacggaatta gtttcattct tttattcatc atatatcctc
     8641 ctaaaaattt tattgcttat aaaaatcatg aattttattt tataataggt ttataaatca
     8701 aattaaaacc tatatctact tatttatatt tatgatatat tttttatttt taggagtcat
     8761 atttttttgt atataaaata atatttagat aactatatta aaaaatacat gtttgcttat
     8821 atacaaaact tactaaaata tctttaatat ttttgtatac aaacagtata tttttcatac
     8881 aaaataaaaa ggatacctca ttagtgctat aacacctaag atatccaatt aatattttct
     8941 taattttttc taattttcgt aaatatagtt cactatttta aaactgctcc tgtatttgct
     9001 gatgttacca atttagcata tctacttaag tatccatgct ttattttcgg ttctaaaggt
     9061 ttaaatttta attttctatt ttctatttca acttcatcta cttttaactc taattttttg
     9121 tctggtatgt ttatagaaat tatatctcct tcttcaacta gtccgattaa accaccttcc
     9181 atagcttctg gagaaatatg gcctatagat gctcctctag ttgctcctga aaaacgacca
     9241 tcagtaagaa gtgctacatg cttatcaagt cccattcctg caactgctga tgtaggagat
     9301 agcatttctt tcattcctgg accacccttt ggaccttcat atcttataac tataacatca
     9361 cctttgttta tttttttacc aaaaatagca tttacagctt cttcttctga attaaaaact
     9421 cttgctggtc cttcatgaac taacatttct tctgcaactg ctgacttttt tacaacagct
     9481 ccatttagag caagatttcc tttcaatatt gcaagcccac cttggttact atatggattc
     9541 tttaatgtat gtattacttc ttcattttct atttcacaat ttcttatatt ttcccctata
     9601 gtcttgcctg ttacggtttt gcaatctaaa tttattccat ttatctttga aagttcgttc
     9661 attatagctg gtattcctcc tgccatatgt aaatcttcaa tatgatgttt tccacttgga
     9721 cttaattttg ttaaacaagg agttttttca cttatttcat caaaaaaatc taagttaaac
     9781 tctattcctg attcataagc tattgcaggt aagtgaagta ctgtatttgt agaaccagcc
     9841 attgccatat caacagctat agcattttta aaagcatcta ttgttaaaat atctctaggt
     9901 tttatgtcgt tcttaagtaa ctccataaca tacatacctg catattttgc tattcttttt
     9961 ctttctccag aatgtgacgc agcagtacca ttatatggta tacccatgcc taaagcttct
    10021 gttaaacaat tcatgctatt tgctgtgaaa agtccagagc atgaaccaca tccaggacaa
    10081 gagttcatgg ctaactcttc taattcttct tcacttactg ttccatcttt acatgctcca
    10141 acaccctcca ttgcagagtt aaaatcatat acttttccat tttttttacc tggaagcata
    10201 ggccctccac tcacaacaat acttggtata ttaagtcttg cagcagccat taacatacct
    10261 ggcactatct tgtcacaatt aggtataagt accaaagcat caaaaccatg tgccatagcc
    10321 atagcttcta ttgaatctgc tattaattct cttgaagcca gggaataatt cattccgaca
    10381 tgccccatag ctataccatc acatacccct atagcaggaa attctaatgg caaaccacca
    10441 ctcattctta ctccattttt tgcagcttct gctatttcat caagatgcag atgaccagga
    10501 atcacctcat tttgtgcatt tactatacct attaatggtc tttcaatttc ttctttagta
    10561 agacccattc catacattaa cgctctcttt ggagctcctt ctattccttt ttttatatca
    10621 cttctcataa tattacctct ttcttacttt tttaaatttt cataatatta ttataatata
    10681 tttttctatt aattaattac gaaaattgta aaaaaatatt agcatatact aatgaaaatt
    10741 tattacgatt ttttatcaat atcttacaca atcgtattat ataatattat cttttcaata
    10801 tattttataa actaatatat tttacaaatt gttgagtaat attttatgat ataaatcaga
    10861 agaagcactt ctataataga aagtgctcaa ctatgaataa gattaataat atatttgcct
    10921 tagatatata gacttttata aatattaaag attaatcaaa tctgatgttt gaaactttat
    10981 cccaattaat cttccttgaa tagtcttctg gatgtgataa tctaactata tgagttgaat
    11041 tatcatagct gtaataaatt gtagtagtac catcaactgg attctttttg tatccttgat
    11101 atacagcact atttggtact ttcttttcaa caaagctata tgcagatgaa gtatcaatag
    11161 tttctatttt attaggaaat ataaacatat aaaaagctac tactataaaa atacatatgc
    11221 atgtaatact ataccattta atccatttca taaaattcct cctactttac caaaatatac
    11281 tattattatt tccatatatt tataatattt atacatacga taaaattttt atatacctaa
    11341 ctatataatt gtagaaactt tgattatata tattttttaa tcttaataat atatttttat
    11401 aaattaataa atttttaaga ttttattaaa agatttttaa aacttacagt ttaatataaa
    11461 gatagtactt ataaaactaa aaacataaat ttgaaagggg acaaaataaa atgatatgga
    11521 cattttattc atttatttgc gttatattac catgcttaat ataccaaata gtcgttataa
    11581 aacgaaaaaa tttgaaagtt gaaaagaatg taatgataca tttagtatgg gtatatattt
    11641 ttttattata tatctatcta gcacttagta aagctggaat tggtagcata tgggatatag
    11701 gaaggtatag tgggcttttt agaattgatg aaataaatct aatacctttt gactcagttg
    11761 gcatcttaac atatatttta aatataatta tgtttatgcc tttaggattt ttactacctt
    11821 taatttggaa aaattttaga aatattatca atgtttcatt aactggatta ggtttttcat
    11881 tagcaattga actttgtcaa ttgtttaact taagagctac agatattgat gatttaatga
    11941 tgaacacatt aggtgctgtt ttaggatatt ttatatgggt aggtgtaaat aatttattta
    12001 atcttataaa gaaaaaagat attttcccat ctatatataa aaatattatt caagatgaaa
    12061 ttgctattac aattgatgat gacataagca aaaaaattac ctctctttac aaaaatgaag
    12121 ctattatata tttaatactt gctgttttag gagaattttt attgtttaat tggtccatta
    12181 taccttatta aataatttat tgataaaaca agtgtacttc gatatttaca aatatgtaaa
    12241 gccctctttc atactacaaa agaaagtggg ctttagttat taaactaaaa tgcacactca
    12301 ctatttagct ggcatctgaa atattttatt aacattgtat ctgactttat ttgatgttat
    12361 ataatcatat tttgttccat tttcaatgaa gttttgtata ttgtccaaca cttgtatagc
    12421 tcttgtatta ttttcataat ctcctatttt tacagtatca attttacttt caaatactgc
    12481 ataaacttga ctaccatcta ttttaatttg gtttatctgc attaaattag ttttatcttg
    12541 acttctaatt attatcatat caattcctcc atttacttaa ttcattttaa tttctaagaa
    12601 ataatatttt ataattgatt tatagattat ttaaattttt aaacttagta ttttattttt
    12661 taatacgatt atttatataa attacatctc taataaaaaa ttctcttaaa gaatactttt
    12721 ttgataatac tttgttttat ttgtagattg catttttgac catta
//
LOCUS       DACTGW010000103        12736 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.103, whole genome shotgun sequence.
ACCESSION   DACTGW010000103 DACTGW010000000
VERSION     DACTGW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.103"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(80..1111)
                     /locus_tag="I9573_002201"
     CDS             complement(80..1111)
                     /locus_tag="I9573_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAT4796818.1"
                     /translation="MLKKKSSLTLKTIREILPGLFISVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGAIVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            1299..2189
                     /locus_tag="I9573_002202"
     CDS             1299..2189
                     /locus_tag="I9573_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT4796819.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSQKYPDIYVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYNSHLLKDFSFDKLQDQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            complement(2241..3167)
                     /locus_tag="I9573_002203"
     CDS             complement(2241..3167)
                     /locus_tag="I9573_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HAT4796820.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFNYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIGKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYILSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVENSLHRILEHSKEGRTSMLQDVEAHRPTEVDMF
                     SKNICKLGKKYSIPTPINQTLFYMIKVIESRF"
     gene            complement(3382..3966)
                     /locus_tag="I9573_002204"
     CDS             complement(3382..3966)
                     /locus_tag="I9573_002204"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAT4796821.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            complement(4051..4953)
                     /locus_tag="I9573_002205"
     CDS             complement(4051..4953)
                     /locus_tag="I9573_002205"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAT4796822.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            complement(4946..5638)
                     /locus_tag="I9573_002206"
     CDS             complement(4946..5638)
                     /locus_tag="I9573_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAT4796823.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            complement(5677..6597)
                     /gene="pyrB"
                     /locus_tag="I9573_002207"
     CDS             complement(5677..6597)
                     /gene="pyrB"
                     /locus_tag="I9573_002207"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAT4796824.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            complement(6893..7915)
                     /locus_tag="I9573_002208"
     CDS             complement(6893..7915)
                     /locus_tag="I9573_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAT4796825.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            complement(8842..9975)
                     /locus_tag="I9573_002209"
     CDS             complement(8842..9975)
                     /locus_tag="I9573_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HAT4796826.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGVEYYDSLLND"
     gene            complement(10306..10962)
                     /locus_tag="I9573_002210"
     CDS             complement(10306..10962)
                     /locus_tag="I9573_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HAT4796827.1"
                     /translation="MYFLPPHDYKLEELDWIFGHELMYFKSKDLHLKYLVLFLKIVYW
                     FNPFIYIMEKYMDLDCELYCDERVLKNCTVQQKQEYALTIVNSMKRGINFSNKFVTGL
                     NKQSDIRKRVMNMFSEKHKNGILMALIISLFSSITFFKFDSTSLIEENDFLERSRYAF
                     EGRQEDMFVTIDSTYADAPIKLRKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK
                     "
     gene            complement(11155..12420)
                     /locus_tag="I9573_002211"
     CDS             complement(11155..12420)
                     /locus_tag="I9573_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HAT4796828.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
BASE COUNT         4161 a         2231 c         1306 g         5038 t
ORIGIN      
        1 attttttatt tatattataa attattagta tcatattatt ttaattaatc aaattaatat
       61 tacaaatact atttttgtgc tatataaata tttttattaa aactacagct gatacaactt
      121 gtactgtacc tacaaataaa ccatataaag aaacttcttt cccttgtttc actaaatctt
      181 ttacattaac ttttaatcca attgctgcta aagctattat ttctagtttg ttacttattt
      241 ctttgcacaa cacagatact tcttttggta taatgttcat tgaaaaaagt gcacaagtta
      301 taaagaatcc aattacatac catggtatag ttactttttt cttttttatc tcatcctttt
      361 cttcttcaag gatttcccta ttagactgat ttttcatatg tccaaatact aatacaacaa
      421 caactagaaa tattactctt actatcttaa aaatagtagc caaatctttt acattttctc
      481 caacaattgc tccacttgct acaacctgac caattgattg caaagttcca cctatcattg
      541 ctgaagtttt tacaagttca tggctatata aaaattgaga tattattgga agtaaaaaca
      601 taaggaatat accagtaaca tttacaatag ttactgcaat tcccttttct ttatctgtcg
      661 cattaaccac tggaactatt gctcctatgg cagaagaacc acatactgca tttccacttg
      721 ccattagaaa tctaaagttt tcttcaaatc ctagtttttt acctatataa agtgcagcta
      781 ttattgttat tgtcatttgc aatattataa ataaaattcc tgaaactttt aagtctatta
      841 aggtagatac actgagtgtt gcccctaata gtactattga atatgataac aaatctgtct
      901 ctgaaaattt ataacctttt tgaaatacct tttgatttaa aaataggttt cctacaaaca
      961 tacctaaaaa tatagaaatt gatgctgccc ctaccttagg aattaaaata gaaataaata
     1021 tactaatata acctactaac actgatataa acagtcctgg taatatttcc cttatagttt
     1081 ttaatgtcaa actgcttttt ttctttaaca taatatacct accttataat taatatagtt
     1141 ttcttcatag caatagtata taattgtaat aaccataagt aaaataaata tataatatcg
     1201 taatgattaa tgtttttaat ggttattata tatagtataa ttatctgtag aaagtatata
     1261 aaaaatgtaa aatttcatta ggtgaaggag acaaaaaatt gtttgaagaa ttaaaaacat
     1321 ttgtagctgt tgttgaatac aaaaacttta ctaaagctgg ggaatactta aatctatcac
     1381 aaccaagtgt aagtaaacac ataaaaaatc tagagaatta ttttaaagtt gtgcttataa
     1441 acaggtctat taaacaaaaa accattttta taacagaaag tggacaaata ttgtacaaaa
     1501 gagctaaaga aatattaaat cttttaaata ttacttatca tgatgtaagc caagtttcag
     1561 atgctataac tggtcactta aagattggtg caagtttaac tattggagaa tatattctac
     1621 caaatttttt agcattattt tctcaaaagt atcctgacat atatgtagaa gtttttataa
     1681 aaaatacatc aatcgtatct tctcatgtta aggattatat tttagacatt ggtcttattg
     1741 aaggcacttg ctcttcccct tcatttattc aagaatattt ttttgaagat aagatggttt
     1801 tagcacttcc atacaatagt catttattaa aagattttag ttttgataaa cttcaagacc
     1861 aaaaatggat agtcagggag gatggttctg gaaccagaga ctatttagat atgtttttaa
     1921 gtgttaaaga aattattcca aaaagtatga tggtttttgg aagtaattat gctgtcaaag
     1981 aatctgttag aaataattta ggaattacaa tagtttctaa tctagtgaca agcttacctg
     2041 ttttaaacaa tgagctgtct gtcatagagc ttggaagcag ctacaacaga catttctcat
     2101 atatatttcc aaaagatata actttgtcaa aagcagcaac tattttcatt gaagagttaa
     2161 aaatatttag taacttaaat agtatataac attaataaaa tttaaaatca ataattatca
     2221 atatttctta caaataccct ttaaaatcta ctttcaatta cttttatcat ataaaataaa
     2281 gtctgattta taggagtagg tatgctatat ttttttccaa gtttgcagat attcttagaa
     2341 aacatatcta cttctgtagg tctatgagct tccacgtctt gaagcattga tgttcttcct
     2401 tcttttgaat gttctagtat tctatgtaat gaattttcaa catcatcttc tgtaagagat
     2461 atgtcttttg cttgtgctat agcaacaact tccctcattg cagattttgc taattctctt
     2521 aaatgctcag aactctgaaa aactccataa ggtgcaccaa gtatagctga agtttgattt
     2581 acaccaatat taaccatgta cttccaccac atatctcgtt gaatatcttt tgataaaata
     2641 tattctatat ttacatcatc aaagacttca gttattatat tagtttttct atcttcacta
     2701 ttatctttgt taccaaatac aattatccca ttagtagtgt gtataatatt attattgatt
     2761 ttctttgcat ctatattggt aacatatgaa tataccattt ttccaattcc aaatctttct
     2821 cctattatct cttcgctatc aactccattt agcagagaca ttataactgt attttttcca
     2881 actaatccat ctaattcttt tatattttct tttaaattat tatattttag acctataatt
     2941 ataaaatcag cctcttgttt atactcatct ttagttacat aattaaaatc ataccttttt
     3001 ccatttatta taaatccttc ttctaaatat cttttctttc tttcattatc acaaagaatt
     3061 ttaaaatcat atttagagtt aaaaaattgt tctgcaaaag tagctcctac agcacctact
     3121 ccaaaaaata caaccttttt atgattttca tttaattgat ttttcatact tacccctccc
     3181 taatatataa tacttttacc ttatacttat attgtactgc tactatctct tattttgtaa
     3241 atatattttt aacaaaaaaa acagctaacc ttcatttata aggctaactg ctttttattt
     3301 ttaagttata tgttatatta caaattttta ctctaaaaaa tttacatttt ttaagtaaca
     3361 ctataaataa ttaagttttt attacatccc taattccttg aactctctac ttccaggctt
     3421 aacaactggt aattttcctt ccttacataa aggacactca tcagattcat aaacttgaat
     3481 atcaagtttt atagcactat atataggcat acctatatca tgattagttc tatctgctat
     3541 acatgcaaca cctacaactt ctccacctaa ggcttctaat actctttttg tctccatagt
     3601 tgatttacca gtagttacaa cgtcttcagc aattattatc tttgctcctt ttttaacttc
     3661 aaatcctctt cttaactcca ttgtattgtc ttttctctca gtaaatacag cttcttttcc
     3721 taattgtctt cctaactcat aagaaactat tactccaccc atagctggtc ctactactaa
     3781 gtctatatct aagtctttta tttgttcaac aactgtactt aatacctcag cagcatattc
     3841 tggaaatctt aatacttttg cacattgtac gtatctatta ctgtgttttc ctgaagataa
     3901 taaaaaatgt ccctccaaca atgcatcact tttctttaat atatccacta catctacttt
     3961 actcatatta tttttcctcc atgttaatgt tataaaatta ttgtttattt aatctatatt
     4021 ttttatcttt aaaatactat ttattctacc ttatataatt cctcttattt cgtctaaact
     4081 ttttattcct tctctatcca taaattcatt aatcccatct attatttcaa tacctattct
     4141 aggattcata aaatttgcag ttccaacttg gatacaagta gctcctgcca ttataaactc
     4201 tatagcatct gtggctttag ttattccgcc cattcccatt acaggtatat ttacattttt
     4261 acacacttca tgtaccattc tcaaagctat aggttttata gctggtccag ataatccagc
     4321 atatacattt tcaaatactg gtcttctatt ttttatatca attgccattg ctttaaaagt
     4381 attaactaat gaaactccat cagcaccttc ttcttcacaa acttttgcca ttcctacaat
     4441 atcttctgca ttgggtgata attttattac taaaggcagt ttcgttatgt ttcttacctc
     4501 acgaacaact tctcttgcca cttcattttt tataccaaaa gccattccac cagctttgac
     4561 atttgggcaa gatatattta attcaataat gtctataggc ttgtcattta acatttgaac
     4621 ccctaataag tagtcttcta atgtcccacc tccaacatta gctattctga ctaagtctag
     4681 tttactaaaa aaaggtaatt catagtctat aaatccttga actccagggt tttcaagccc
     4741 aacactattc atcataccgg atggtgtctc atgaactctc ataccattgt taccaggttt
     4801 tttatttaaa gtaagaccct tgctacttat accaccaagt ttttgtatat cataaatctc
     4861 attatattct tttccaaatc caaaagttcc agacgccatt attactggat ttttaaattc
     4921 tacacttcca aatttcacac ttaaattacc cattaaaaat cacgtcctct ccccagaaca
     4981 ctggaccatc tttacaagtc tttttgtttc ctccattagt tttacacgta catactaaac
     5041 aggcacctac tccacaagcc atatggtttt caagagatac cataattctc gttttagtac
     5101 cttctaccat cttaactaat ttttccatca ttggagttgg accacatgtt aatatataat
     5161 catatttctc aacatcaatt atatctgtta caaaagtatt tcctactgtt gtatagactt
     5221 tattacatac ttgcttatat tcatcttcta atataacatc ttctctaaac ccaagatatg
     5281 catcacaatt ttttatattt ttagcaacca aatatagtgg agctacaccg attcctcctc
     5341 ctactagagc tacctttcca tcaaccttag catatccatt accataaggg ccttctaatt
     5401 ttatattatc atttaccttt aggttactca aaatttgagt tccttctcct actactttat
     5461 aaagaaaagt tatcccttct tcatctatat catgtatact tataggtctt gaaagtactg
     5521 gataagtatc ccatgctctt aacatataga attgtcccat tttaccttca aaattacctt
     5581 ttactttcat tctatacata tcttcgccta tatatatatt ttccagaatt ttatacatcc
     5641 caaaatctcc tttgttttac tattatatga taactattag tcttcattta tacctaaaag
     5701 ttttattatt aaagccattc taacatacat tccatattta gcttgtttaa aatatacagc
     5761 tctatcatca ctatctacat ctatgtctat ttcatttact cttggtaatg gatgcattac
     5821 taacatatct ttagatgcct tttctaattt agctttattt aaaatataat agtttttaag
     5881 tctttcatac tctgatttat cttcaaacct ttcttgttga actctagtca tatataaaac
     5941 atccaagctt cctataacat catccaaatt attagtttca taatatgcat gtccctttat
     6001 agcttctttt atataatctg gcatttttaa ctcttcaggc gctataaata caaactttgt
     6061 atttttatat cttgccatag ccttaaccaa agaatgcaca gttcttccat attttaaatc
     6121 accacataat cctattgtat ggctttctaa agttcctttt aatgatttta tagtaagtaa
     6181 gtctgtaagt gtttgagttg gatgctggtt tttgccgtct cctgcattta taaatggcac
     6241 ttccgtatat ctagctgctt ctgaagctgc acctgattga ggatgtctca tagctattat
     6301 atcaacataa ccagatacta tcctcattgt atctcctaga gtttcacctt ttgatgcaga
     6361 ggatgaattt ggctctgaaa agcctactac acgtcctcct aatctgttca tagcagattc
     6421 aaagcttaat cttgttcttg ttgatggttc ataaaataat gtagctaata gttttccttc
     6481 acatatacga gaatattttg acggattgtc aattattttt tgagctaatt ctaatatttc
     6541 atctatttct tctattgaaa agtcttctgg ttggattaaa tttcttgatt ttaacatctt
     6601 tcgtcctcct tttttagcct ctctgaacta aaattaaaag tatttttgta ctgttagtag
     6661 cttaccacct ttaccctatc ctgtcaaggg attgcgcaaa taatcatcat atgaaaaggc
     6721 tcctatttaa tagtcatatt aacttgaatc ttgattagat ttaaagtgat acaaaaacgc
     6781 tctatagtac atttaaaata taatcatttt ttgtttccat gattaaatta aatataatat
     6841 agagcgttta tatatattta tgtatttatc aatttgtcat attacaaacc gattataata
     6901 ttctttttgc tgtaacaaat cttgctgtat agtatgatga atttatacta gttacactaa
     6961 ctgtttttcc tgttgatggt gaatgtatca tattaccccc acctacatat attccaacat
     7021 gactaacaga accagaacca ttagttgtga agaatactaa gtctcctggt tgtaaatttg
     7081 ctttacttac agtttgtcca tatttagatt gtgctttaga tgttcttggt atattctttc
     7141 ctactgcatt tctatacaca tattgtgtaa atccagaaca gtcaaaagtg tttggtcctt
     7201 ctgctcccca aacatatgga caaccttgct tagaatatgc caagttaagt acagcttgaa
     7261 ctgcattaga gttagctgtt ggcacatttg aattattatt ttgttgatta gtatttgcat
     7321 tgttttgttg attattattt gtagaatcta cattttggtc aatacttggg tttacttgtg
     7381 gctcttctat tacaggagct ttatcagata tataagatga ttttacatat ccttctgttt
     7441 ccccatcttt aacttttaac caatctccat tatcctccaa aaccaataaa gaacttcctt
     7501 tttccagctc ttcaattact tctgaacttt cactcttatc ttttcttaag tttactttat
     7561 cagcgttagt atatcctaca ccttcagata tatctacata tctagtagct aaccaacctt
     7621 cgccatcttc aactttaacc ttaacccatt ctccttgagt tcctaaaacc gtaaattcat
     7681 ctccagaata agctatgttt tgaacttgac cttcttctct tatctttata gctactccat
     7741 cttttatttt tgcaacttta tattttactt ctttatattc acctaaattt aattcagtat
     7801 ttgttttatc atccttattt tgagatgaat catttacttc atctgcattt acaattgaac
     7861 ttactgataa tgccataact gatgcaaata ctggtattaa tatctttttc ttcacgataa
     7921 acctcctaaa attttcccat aattatctta tgattctatt ttgtaggtga agtgtaccta
     7981 aggataaaat actaatacac aatacattaa taaaagctct atctgtaact tttttgtaaa
     8041 ccactttata taacttttat agtatcatta tattaataaa ataaataaat tagcttatcc
     8101 aatatgtcac ctaaatatac attatttact taattatata atatgggtct gtaaaagtaa
     8161 agaattttgt accattcgag gttaaagtgc gcataaatat tgttttatgt gtttttttat
     8221 gcgcttttat atggcgtata ttttttgatg gatttagtat aaagttagat attctgtgaa
     8281 atttttaacc ctgcataaat caaaacctat ttcctaatag tgattctcaa attctaataa
     8341 ttctaagtta aaaagatgaa aattttggag aatataaata ttaaaaaatt aagtatgaaa
     8401 agattgtcct agcagtaatc ttttcatact taattttttt agataatata tcaatagcaa
     8461 ctatcattaa tatacaatag ttaaattttt tactaacttt tttaagtaca ttattctcaa
     8521 agattaatat aagaagaatg cttaaactac ttactataac aactttgaaa attctatttt
     8581 aaatgaagtt caacatacac ttttccgaat tgtgttttat agtattttac acctgaattt
     8641 atctaatttt ttaaaattat aaaccttata attaataatt tatttcactt atttaagttc
     8701 tttataagta tatccttgtg tactatctta actatttttt attcttggta tataagtatc
     8761 tatttttata aggatatatt tatttctcaa caaattacta ttcaaaactt tataattaat
     8821 atctgttaaa ataatattta tttaatcatt caatagactg tcataatatt ctacaccaac
     8881 ttctatttta tcagagtctt taggtatcaa acctaatgat tcacaattta ttttatattc
     8941 ttccctcata tttttaggag catctgcata tgtaaatcct ataactgaaa aacgtatact
     9001 gctaacagtt cgtaagtctt caaatccttt aggtaataca ttaagattaa caatactcgt
     9061 tgaattaacc ttcaaaaaag taatagaaga taataaacat aaagttaatg ccattaatat
     9121 accattcttg tacttttcat taaacatatt cataactctc tttttaatat cagattgctt
     9181 atggagtcca gctataaatt tatttgaagt atttgaacct tttctcattg catttagaat
     9241 tgttaaagca taatcctttt tttcctctga atttcgattt tttaaaactc tttcatcaca
     9301 ataaagttca caatctaaat ctatatgttt atccattata tatataaatg gattaaacca
     9361 atatactgtt tttaaaaaca ggattacata ttttaaataa aggtccctac tcttaaaatg
     9421 cataagctca tgtctaaaaa tccagtctaa ctctttcaat ttatactcat gtggtggcaa
     9481 aagtacacat gaattaaaaa atgctggtgt ttcaacttca taagagtatt tgagttttat
     9541 atcttttgtt atattaaatt cttctaatat atttttataa agatttacaa tctctctatc
     9601 atcaacatca taagataagt catttattaa atttttaagt tttatatatt taaatatagt
     9661 gtaaattaaa tacactacta cagttataat ccaagtgtac aatataatat tattacttat
     9721 agttgaaaca ttattaaatc cctctaaatt tatgttatta atattaatgt gttttttact
     9781 tctcaagata tttattgtaa aagtataata tgtaaatggt aaaaataatt taataacaac
     9841 tatcatccat atataataat taaatttttt gctaaatctt ttaaatacat ttttcttgaa
     9901 aatcaatatc agaagaatgc ttagactact tactataact gttctaaaaa tcccatttag
     9961 aataaagtta gacatacatt tttctcctat cttatttaag agtattttat gtttaagttt
    10021 atttgccttt taagttttca tattatcttt attaattaaa aagatacatt ttttgtaaca
    10081 gataactatt ttttatcatt ttataattgt gtaactcata atttatttaa actcccctat
    10141 aattaattat agcttagtaa tgattcctat gcatcaatca tatttaatta tttcaactat
    10201 ctataaattt catacttaaa cacatataaa attgaatcta taataaaatt ttcaattgct
    10261 taaatataaa aaattcataa tatcaacatc aatattaagt ctaacttatt tttttaaatc
    10321 catacaatct ttataaaatc taggaataat ttctattgaa tctgaatctt ttggtactat
    10381 tccattgact ctacagtttt cttcatgctc ttttctaagt tttataggtg catctgcata
    10441 tgtagaatct attgttacaa acatgtcttc ttgccttcct tcaaacgcat atcttgacct
    10501 ctctaagaaa tcattctctt caataagact agttgaatcg aatttaaaaa atgtaataga
    10561 agaaaacaag cttataatca aagccattaa gataccattt ttatgttttt cactgaacat
    10621 gttcattact ctttttctaa tatcagattg tttatttaat ccagttacaa atttatttga
    10681 aaaatttata cctcttttca ttgaatttac aatagttaga gcatattctt gcttttgttg
    10741 tacagtacaa ttttttaaaa ctctctcatc acaataaagt tcacaatcca aatccatata
    10801 tttttccatt atatatataa atggattaaa ccaatatact atttttaaaa ataatactag
    10861 atactttaaa tgaaggtctt tgcttttaaa atacataagt tcatgtccaa aaatccaatc
    10921 taactcctct aatttgtaat catgtggtgg taaaaaatac attcaaaatt aaatcaacca
    10981 ttaattttca cccatcttat ttatgcattc taaattacat aatttatcaa gttatttata
    11041 agttaaaaca aaaatagctt acttcattgt aagccacata atttaagggc tgtactatgt
    11101 acagcccctt tatatcttaa ataattatat cctattattt attaagagta aatcttagta
    11161 ccatcctctt aatttcatag cttcagcaac tttcttaatt gaatgcatgt aagcagcttc
    11221 tctcattgta acattgtact cttctttgat tttccaaata gattcaaatg ctttaaccat
    11281 agctatttct tctttttgct ctacttcttc ttctgaccag tagtatccat ataagttttg
    11341 tacccactcg aagtaagaaa ctgtaactcc accagcatta gttaatatat ctggagtaag
    11401 aactattcct ctttcagcaa atacttcgtc agcctctgga gtagttggtc cattagcagc
    11461 ctcacaaact aatttagctt ttatagattc agcaacttct ttagttatag agttttctaa
    11521 tgcagctggt attactatat caacatctga agcccagaac tcttctaaag atattctctt
    11581 agctcctggg aagtttaata agttaccatg ttctttcata taatctaaca tagcttgacc
    11641 atctaaacca ttttcattgt atatagcata agaaccttct gatttacacc attcagccat
    11701 agctacaaca gtaccaccaa gtttttcaca gttaagaact gtataagaac caacgtttcc
    11761 tataccttga acagctattt tagctttttt catgtctatt cctaatttag cagcagcttc
    11821 tcttgcagta acagcaacac caaatccagt agcagctgtt cttcctaaag aaccaccaaa
    11881 ttcaactggc ttaccagtta taacacctat agaactttgt ccagttaatt tattgtactc
    11941 atcaaccatc caagacatta tttgaccatt agtatttacg tctggtgcag gaacgtcaac
    12001 tttttcacct attaatttat atattccatc tatgtatcct ctacttaatc tttctaattc
    12061 accttgagat aaagttgatg gatctactat tattccacct ttacctccac catatggtat
    12121 acctgttaca gaacacttga aagtcatcca tatagataaa gcttttactt cgtctcttga
    12181 aacattttga tggaatctta ttccaccttt tgttggccct actgcatcat tatgttgtga
    12241 tctaaatcct ttaaaagttt ttatagaacc atcatccatt tttactggaa ttgaaacctc
    12301 tataactctc ataggttctt ttaataattc ataaactgct ggttccatac ctaatttatc
    12361 acatgcattt tttacttgag attgcgccat ctcgaagaca tttacatctt ttcctgacat
    12421 ttgaaagccc ccttataaat acgttataat tatgtatact ccatttagaa aaaaccattt
    12481 cctaaattta cagtttaatt atagcacact ttattgcaaa ttagtatatc attttctcag
    12541 tttatttttt aacattggag gaagtttatt tttttaattt tatgaatgtt aatatctatt
    12601 ttttgtacat taactaaagt cattttctca atagactttt ttattgtctg ttgtatgtct
    12661 tcaatttttt ttatagtttc taaatcattg atatttatac ttacaaaaat attcataaaa
    12721 ttattgcaat tctcta
//
LOCUS       DACTGW010000104        12597 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.104, whole genome shotgun sequence.
ACCESSION   DACTGW010000104 DACTGW010000000
VERSION     DACTGW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.104"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(348..1304)
                     /locus_tag="I9573_002212"
     CDS             complement(348..1304)
                     /locus_tag="I9573_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAT4796829.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTIYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIQAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            1745..2113
                     /locus_tag="I9573_002213"
     CDS             1745..2113
                     /locus_tag="I9573_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAT4796830.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNKVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
     gene            2538..2783
                     /locus_tag="I9573_002214"
     CDS             2538..2783
                     /locus_tag="I9573_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796831.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
     gene            2882..3145
                     /locus_tag="I9573_002215"
     CDS             2882..3145
                     /locus_tag="I9573_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HAT4796832.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(3430..4377)
                     /locus_tag="I9573_002216"
     CDS             complement(3430..4377)
                     /locus_tag="I9573_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAT4796833.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALATEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(4380..6011)
                     /locus_tag="I9573_002217"
     CDS             complement(4380..6011)
                     /locus_tag="I9573_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT4796834.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            6141..7058
                     /locus_tag="I9573_002218"
     CDS             6141..7058
                     /locus_tag="I9573_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT4796835.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            complement(7115..7696)
                     /locus_tag="I9573_002219"
     CDS             complement(7115..7696)
                     /locus_tag="I9573_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HAT4796836.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(7736..8302)
                     /locus_tag="I9573_002220"
     CDS             complement(7736..8302)
                     /locus_tag="I9573_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HAT4796837.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            complement(8757..9617)
                     /locus_tag="I9573_002221"
     CDS             complement(8757..9617)
                     /locus_tag="I9573_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAT4796838.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            9766..10656
                     /locus_tag="I9573_002222"
     CDS             9766..10656
                     /locus_tag="I9573_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT4796839.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLSPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            10874..>12597
                     /locus_tag="I9573_002223"
     CDS             10874..>12597
                     /locus_tag="I9573_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HAT4796840.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDVTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNVKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVSVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLVGNGINKLPEGWSKDYILGNG
                     QPIIEVKN"
BASE COUNT         4628 a         1734 c         1792 g         4443 t
ORIGIN      
        1 tacttttaaa cgggttagac tgtaaatctg ttataagtat attttttaat tcctctgtaa
       61 gttcagaaat taaactatca cctatgtatt tttttatgat ttcctctcta atttctttta
      121 aatttagcaa tattagtacc tgctacttac atagtagctt ttcctttcct tttaatacta
      181 ttattcattt ttgaatctta tttagatata ttttaagatt aaaagtcttt agctatataa
      241 ttttttaccc atttttaact tatattaaca tattacaggc ttatatctcc accctgctta
      301 cattgaggat ggagtatatt gcctattttg ataaaaatga taggttgtta tttgcctatt
      361 cctttttctg ataatttttc taacatatct ttggtcattt tatctaaatc ataactaaac
      421 ttaaagttcc attcttttac tgcactacta ctgtctagag agttaggcca agaatcagct
      481 atactttgtc ttacagggtc tacatcatat tctatcacaa aatcaggaat atattttttt
      541 atagaatcag ctatttcttc aggagaaaag ctcatagcag taatattaaa agcatttcta
      601 tggatgagtt tatccgctgg agcttggatt aaatccacta ttgattgaag tgcatcaggc
      661 atatacatca tatccatttt tgtaccttct gctatgtaag atttatatct cttatttttt
      721 aatgcctcat aatatatatc aactgcataa tctgtagttc ctccaccagg aggcgtaaca
      781 taagaaataa gacctggaaa tctaacacct ctagtgtcta ctccaaattt tgagtgataa
      841 taatcacata gtaattctcc agaaacttta gtcacaccat atatagtatt aggtctttgt
      901 aatgtatctt gaggagtcat attttttgga gaattttcac caaaagcagc aattgaacta
      961 ggtgtaaaaa atttacaatc caattctttg gctatttcaa ggccatttat taatccattc
     1021 atatttaagt tccaagcttc taggggttta ctttcagcaa ctgcagaaag taaagatgct
     1081 agatgaatta tagtatctac atcatatttt tttgcaattt caaaaaaacg attcttatct
     1141 gtaacatcta atatctcaaa tataccagat tcacatactg gattaccttc ttttactctc
     1201 cttgaactag cgattacatt ttgttctcca tactcatttc taagctttat tgttaattct
     1261 gaacctattt gtcctaatgc acctgttata agtatttttt tcaattttac aacctcctaa
     1321 gggtttaatt tatgtagtac tacttatttt tattttagca aatcttttgt ttaaatacaa
     1381 agaattaaag ataataatat acgacttaat tgaatgaagt gcagtaaatt cctaaaaatt
     1441 taaagttgag atataatggt ctattgtata acaataaaag actagattaa attattatat
     1501 agattgtgtg actaagtcac agaaagtata gttaatttaa tatataataa aagcatatca
     1561 agtaaagctt taaattaaag taactttata taaaaagtaa tataaattga taaaattata
     1621 aatttattta tagtgaatat aggttggatt ttatagatac aaaaatagta tatatgatat
     1681 gtagaccatt agcatgttta aagtagcaaa acatataaaa taaaatttga aaggaagtat
     1741 aaaaatgaca agaccatcaa tatatcatag ccaaggatac acttgtgcag aggcattaat
     1801 aaagtcttat aatgaggaac acaacacaga tataccaatc tcaataggaa gtggtatggg
     1861 ggttggaatg aatgttggaa gtgtttgtgg agctgtaaat gctgctgttg ttataatagg
     1921 gtatttaaat gggcgaaata gtaatttaga tgaaaatata gcaaggaagt attcaagaga
     1981 gctattgaat aaagtaagaa ataaatacag ctcagaaata tgtgtatctc ttaaaaaaaa
     2041 taaagtaact tgtgaagaaa taataaattt cacatatgat gctctaaatg atatgctaaa
     2101 aaatcaaaaa taattctaag aaacaagtat tttacattcg atagaatact tgttttttta
     2161 tgatattttt aataggtatt agtttattat aatttttaaa tcaattttat tatattttaa
     2221 ttgaaataat ctactttgaa aattactaac tgacaaaaga atatgattat agtatagaaa
     2281 aaatgtaaat tatttaatat taagtgagag tatatgctta cctatataaa atcgtataaa
     2341 ttaatggcta aatttataaa ttataaagaa atatataaga aaacaaaaga attaattagt
     2401 ataatataaa gttaaaaagt gaaaatttat ataatttatt tataaagtaa agaaaaaata
     2461 gaaatttgta atgaaataaa aagtttatga cataatatgg tataattagt aaaaatatag
     2521 agattggagg aattgctttg tttatagatg aagaattaga agggtatata ttaacatgta
     2581 aaatatctga agactttaaa aatatacctg aatatagtga tgaagagttt tatgttacag
     2641 tctataaaga tgaaagttct gactctgggt actatgcttt attagaaaat aaagaagaaa
     2701 gagttgtatg ggatggagaa gttgttgcca ataatatttt taataacctt tggattgtag
     2761 taaataaggt taaaactgga taatagtcta attatatagt atttgtaata ttttaaatga
     2821 atttttacat atttgagatg agtaaataac aaagattatt cgatactgga ggagtaattc
     2881 aatggaatat agttatagta agatgaactt aaaaaaaggg gatattgtag aggtaaattt
     2941 agagaaacaa gccaatgtaa tattgttaga ccatataaat tatgtgaaat ttaaaaatca
     3001 aagaaattat gattactatg gtggatttgc aaagaaaaat ccatgtagaa tgagagttcc
     3061 taatactgga acatggtatc tagtagtaaa tcaagatgga aatagtggaa tagtaaactt
     3121 ttcaatcaat acaattcaaa attaatttta gaatattgga tattcttact ttttgtactt
     3181 tttatatagt taaaataaaa ttaataagca attattataa aataatgtgg gtgcctcaaa
     3241 atgaatttta taattcacga gacactcttt tttgtataaa ctcaaatata ctttatcatt
     3301 tcaacaatga aaaagaggcg tatctgtgtt ttgagatagc ctcatttatc atatatcata
     3361 tatagaattt taattattct taactaaaat tttttccata ggttatctgt acaattaaaa
     3421 tatttattgt tagcatacag ctccacctgt aaggtgtaaa agagcctcta gtatctcttc
     3481 ttttctttcc aaaacttcat ctgataaaag catttctata aatttatcag caccaattct
     3541 atcgatagtt attccaaaac gttcacctgt gttaccctgt tctctaaata aaagaattgc
     3601 tttttcaatt aaagtcataa gttcctcttt tgaaaataat ctatcaatct gtgttccagg
     3661 acgaactgat tttccccatt tccctcctat atagacttta taaccagatt ctttttcttc
     3721 aatactatca aaattacaac tttctataca cttaccacag ttattacata gattactgtc
     3781 tatttcaagt ttacctttat ctgttaattt agctgcttta acaggacaga cctctacaac
     3841 agcacatttt ttacaaccaa cacaaagctc tgaatcatag tcaggtacac gttgaccaac
     3901 aattccaaga tcgtttaggt caggtttaat acagttatta gggcatccac caacaccaat
     3961 tttgaactta tgaggtagtt ttacatcata ccaatttttg taaaattctt cgtgtatttc
     4021 agtagcaagt gcctgtgtat cagctagacc atgaacacaa acagtacctt tacaagccac
     4081 aacaggacga acccttgatc cagttccacc tgttattaga ttttcgctag caataaattc
     4141 acttagagct tcaatatctt caaattttac accaggtaat tctactgtta gtctagtagt
     4201 aaatgctaac tgaccgtttc cgaatttttc agcagcttct gcaatttttt ttgcttgtga
     4261 tgcatttatt acaccattta cagttattat acgtgctgca aaatgttctc catccctgct
     4321 tggtagaaaa cctctacctt ttacttcctt tttttgttct gctgtaacat tactcatatt
     4381 tactcctcca ttaccttaag atttggataa aatagtattc caccttcaag aactttcgta
     4441 tttgtatagc caaagcgtct taagcgattt tgcgccatat agctattttt acctttagca
     4501 catataagaa gaattttttc atccttagca agaccttcaa tttctccatt aatttcacca
     4561 acaggaatat aacgtaaact aggaatagaa ggagcttttg aaacatcaag tttagtccaa
     4621 ctgtcaaaat cctcaatttc gtcaagtaaa acactatcaa gttctccgtt aagtttattt
     4681 tgtaaaacat ttactgccac tacaaaaggg tgaatagctg ttgaaaaagg tggtgcatat
     4741 gcaaggtcca tactttctaa tgaataaagg tcagcgccta aagttattgc agtagcagcg
     4801 atatctacta ttttatcaac cgctccaggt cccataactt gaacacctaa taattttcta
     4861 gtattttttt cagcaaccat tcttataata aaattagatg cacctggata ataatgagct
     4921 ttatcatcgg tagctatggt tacagagcaa acattataac cttctttttc agctatttct
     4981 ttacttagac ctgtttttcc tgcattgagc tcaggtaatt ttactactgt cgttccaaga
     5041 acaccattgt aagttttaga ttttccagat atattttgtg cgcaaattct accctcatga
     5101 tttgcagatg accccatagg tgaccatgta ggtttatttg ttaaagaatt ttttacaaaa
     5161 gcacagtctc caactacgta tatatcttta tcatttgtaa gcatatattc atctgcaata
     5221 attgtattat taggggcaaa ttctaaacct gtgtctttta gaaaacctgt gttaggacgg
     5281 attccaactg acataaccac taaatctgtt ttaatagctc tatttttagt acgaagtttt
     5341 tcaactttat tttcaccttc aatacctaca acctggtcac ctgtaaaaat cattatgcct
     5401 ttatcagcca tatgattttc aacgtattca gcaaaatcag tatcaaatcc aggcataaca
     5461 tgttcagcca tatcaacaac acttacacgt atacccattg cagaaagatt ttcagcaatt
     5521 tcaagaccaa tgaaacctcc accaataaca acagctctct taataccgtt ttcaatagaa
     5581 tctcttagtt ttatagcatc atttggagta cgcataaaga atactccagg taaatctatt
     5641 ccaggtattg gtggttttat tgggtctgcc cctgtagcaa ttacaagttt gtcatagctt
     5701 agattttctt tttctcctgt tgaaagcgtt ttcaaagtta cttgttttgc atttctatca
     5761 atagacgtaa cttcaactcc acaacgaact tctgctcctg ttagtttaga aaaactttca
     5821 ggtgtattta cgattaaaga tgatttatct tcaattatct taccaacgta atatggaaga
     5881 ccacaacctg cataagaaat atcatcacct ttatttagta ttgtaacaga gcaattatca
     5941 cccatttcac gttttatttt tgcagcagtt tttgttccag cagcaactcc acctataatt
     6001 aatactttca caatatatca cactccaatc tactttataa ctctataata aaattatact
     6061 tcttgacaat atatttgtaa aatactaaaa tattaataaa atcataagca tttactaatg
     6121 tttgctaagg ttgtgataat atgacaattc gtagtcttga aatctttgtt aaggttgctg
     6181 aatgtggtaa aatgagtgaa gtagcaagaa atatgtatat tacccagtca tcagttagcc
     6241 aagctatttc agaaatagaa aaagagtatg gtgttaagtt atttgataga atttcaaaaa
     6301 aattatattt aactgaggca gggaaaaaac ttcttggata tgggaggcat cttttagctg
     6361 taaatgaaga aatgaatgat tgtatgaagc attgtgcaaa aaatatccgt ataagagttg
     6421 gggcaacagt aactgttggt acatgtgtaa taagcccgat aatgttagaa ttatacaagg
     6481 taaatcctct tatagaacct gaagtatttg tagaagatac aagactgatt gcaaaaaaat
     6541 tacttaatag tgaattagat attgcgattg tagaaggaaa aattaagcac ccagatattg
     6601 ttacaaaaag cattattaat gataatcttg tacttatatg ttcacacaag catgagttct
     6661 acaaaagaga ctctataaaa gtttcagaac tttcaaatca accattaatt atgcgtgaat
     6721 taggaagtgg tacaagagca caattagaaa agcaattaaa agaattaaaa atcccaatga
     6781 atattagatg gtcatgttat aactctgaag ctatattaag agcagttgta gataattttg
     6841 gcattgcagt tatatccgaa ttacttattg aggattattt aaaaaagcat cttttgtggg
     6901 cttgtgatat tgaaggtatt aacttacata gaacattcga tattgcatat catagaagca
     6961 agttttttac agaaaatatt tcagcatttt ttgatatttc agtagaatat gggaaaaaac
     7021 aagcgagaaa gaagagtaga agtgtaaaca gtttataata tatctaaata aaaataagag
     7081 tatatcaata tactcttatt tttatttaga tatattagcc tatattaacg ccaaaatttt
     7141 tacacaatcc tgcaagtcca tcctcaaatc cagaacctaa agcattaaat ttccactctc
     7201 cattatgtct atatagttct gccacgacta ttgctgtttc tatgctaaaa tcttcaccca
     7261 attcatattt aataagttcc tcgttagttt cttcattata tattcttata tatgaatttt
     7321 caacttgtcc gaaattttgt cttcttgtaa ttgcttcatt tatagtaaca ctaaatgata
     7381 ttttagcaat attttgaggt attttagata agtctacatt tatttgttca tcatcaccat
     7441 caccaacacc tgttctatta tcacccatat attcaacagc tccagaagcg tgttttaaat
     7501 tattatagaa tataaagtct ccatcattcg ttacttttcc atcagttcca gttaaaaatg
     7561 cagaggcatc taaatcgaaa tcaaatccac catcatattt attaatatcc catcctagtc
     7621 ctactattac tttttttaga ccagggttac tttttgttaa acttactttt tgtccttttg
     7681 ctaaagttat acccatttaa accaactcct cattataaat tttttattgt aattattact
     7741 tatatgtatt aattaattca cgaagctctt cattttgacc aattcctaaa gcattaaatt
     7801 tccattcttc atttctacga taaatttctc ctgccaaaac tgttacttta ccagaaaagt
     7861 cttcttgaag attatattta aataattctt gcttggaatt tgaatcaatt aatcttataa
     7921 aagagttgtt catttgacca aaatcttggt ctcttttttt agctttgtat atagaaactc
     7981 caaaaactat tttatgtaca tattcaggta tgtcttctaa attaacaatt atttgttcat
     8041 catcaccttc accttttcca gttaagttat ctccagtgtg tataatagct ttagaagggt
     8101 gtttaagatt tccaaaataa accaagtcac ctctaacatt cattttatct tggttagtca
     8161 atgctattgc aaatgcatct aggtcataat tttttttacc caatttattc atatcccatc
     8221 ctaagcctac tactagattt tttacaggat tattatcttt tactaagcta actctttgtc
     8281 ctttttgtaa actaacactc attttaagtt ctccttttta tatttttagt tttattgatg
     8341 gtatcaagta taagaaccat attagctata ttatatacgt atttaggcta agttggctat
     8401 atttataaat tatttattat ataactacat gaatatttta taatttaaac atattgacat
     8461 tatatgattt aaattatttt gtagttttat aatatttaaa tttgtatttg attgttataa
     8521 agtaaagtaa gtaataaagt tagactcaat aattctaaaa tttctaagca cactatctaa
     8581 atgagaacgt atttggaaat acttgtataa aacaataatt gatacattta atttttatct
     8641 taaaaataac aattataata tataaataga aagtataata tataaatagc aagtataata
     8701 taacatataa aaagttgaat ttgtatatgt taattatact tgctatttat tgttagttat
     8761 tctatttttt taaatccatt acctaaagct tcatgtatat ctgtaattat aataaaagca
     8821 ttagggtcga cattttttac taattttttt aattcgacaa cttctttctt ttcaactact
     8881 actaaaagta catttttatt ttctccagta tatgcacctt ctccttttag aatagttgca
     8941 cctctatcta aatcttccat tattttttta cttatatctt ttgaaaggtc agatataata
     9001 gtgaatgatt tggaataatt aaatccttca ataagcaagt cactaacttt tactaaaatg
     9061 tataaagcca cagttgaata aagtgcaatt tcaacctgtc tatttactat acctgataaa
     9121 acaactacaa cgcagtctac aatacccatt aaaacaggta tactaaatgt aggaaaatat
     9181 ttatttagta ggagtgccat taaatcagtt ccacctgtag agccatttat tctaaaaata
     9241 agtccttgac caactcccat taaaatagaa ccagatataa ttgcaagata catatcattt
     9301 gttatatcta atgtagccat atttgcagta agcttaagtg ctatggtaag aaaaattatt
     9361 cctaaaaccg ttttaaaaca atcttttttt gaaagtattt tgtaagcaaa tatgaataat
     9421 ggaacattta aaagtaaatt gacaatccaa agttctacac cgaataatga atttaaaaca
     9481 actccaagtc ctgttatacc accagcagcg attgcatgtg gattaaaaaa taaattaaga
     9541 gaaatggcca ttaatataca gcctacaatt aatattaaaa tttctgaaaa agttattcta
     9601 tcttttttca ataacatcac tccttatata tagattataa tatatattat atagtttttc
     9661 caatttttaa taaaagaaat aaaaatatat tataaaaaca atttgattgg aattttcaga
     9721 aaattaatgc tataattaaa agtaaattta aagggaggtt ataaaatggc taaattaact
     9781 ctagaacata taaaaggaaa tacatattat attccactcc ctacaatagt aggtgtatac
     9841 gtagatggta aagatgcaat tttaattgac agtggaaata ataaggatac agctagacaa
     9901 gttttaagat tattagaaga acataattta agtcctaaat taataataaa cactcattca
     9961 aatgcagacc atataggagg aaatgcatat ttgaagaatc aaacaaaatg caagattgct
    10021 acgactaaaa ttgaaggata ttttacagaa aatccaatat tagaatcagc atttttatat
    10081 ggtggctatc caagtaaagc actgaagaat aaatttttat tagcaaagga aagtgaagtt
    10141 gattatatta taccttcaaa tggcaaaatt gtagatacag aacttgaagc gatttctctt
    10201 ccaggtcatt attttgaaat gattggtgtt agaactccag ataatgtttt gtttgttgca
    10261 gatagtctta caccagagaa tataattact aaatatcatt ttttcttcct actggatata
    10321 gattcacagt ttaagacact agaaaaattg agaatgttag aggctgattt ctttgttcca
    10381 agtcactctg tcaaaactac agatataaaa agtttgatta atattaataa gaagaagatg
    10441 gaagaaataa tagataatat taaaaaggtt tgttatgagc cagttatgat tgatgaagtt
    10501 attgaaaaaa tgtgtgattt gtacaatgtc aagcttgatg ctaatcaata tgtgttggtt
    10561 ggaagtacaa tacgttctta tataacttat ttatatgaga ataatatggt tgaatatata
    10621 tttgatggtg gtaagatgat gataaaagct gtgtaaatag taataatttt caaattatat
    10681 gatgaaattg ttttagtatg actgttattt gaaaagtaac ataaaaaaat ttatataaat
    10741 aagctaagat gattgatata aagttactga taattttaag gtaatatata cgttactata
    10801 atattatgtt attatttgta aataagggga taatttataa gcaaattaaa gaccttaatt
    10861 tacaggagga atagtgtata ttatgaataa aaaattcaaa aaattcagta aaaaactaag
    10921 tatattaatt ttagttttga ctatgtttct ttttaataat ttaactgcaa atgcacagat
    10981 taaagaagat tcaataatag gaattgatag atatgaaaca gcaggactta tagcagatag
    11041 acaaacttat gatactgtaa tattagttaa tggagataaa aatttatctg atgggctttc
    11101 atcaagtgga ttagctggag ctattaacgc accaatactt ttgactaaaa aaagtgaaat
    11161 accaaaagct actttaaata gactagataa taagagttta aatgttgtaa aaaaagtata
    11221 tataattggt ggttataata ctattgaaaa ttcagtagaa aaagatataa aaagaaaagg
    11281 tattcaagtg gaaagaataa atggtaacaa tagaattgaa actagttata atgtagctaa
    11341 aaaaataaat gaagtaagta gtgtaaaaga ggtaatgctt acaaatggat ttgtaggaga
    11401 ggctgatgct atgagtatag cacctgtagt tgcaaaaaat aaaggagcta taatacttac
    11461 agatgggaaa tcagttccat ttaagactga aggtttaaat gtttacgcta taggtggcaa
    11521 atctgcgata aatgaggatt tagtaaatga aactaaagca actagaatcg gaggaaatga
    11581 tagatttgag accaataaaa aggtaatgga aaagttttat aatggagcga cagattttta
    11641 tataacaaat ggatatcaat tagtagatgc actaacatta tctcctttag ccaaagaaaa
    11701 accaatcgtt ttagttgctg atggaagtaa taaaggtatt ttaaaaggag ctaaaagtat
    11761 aacaaaagtt ggaggtattg atgccaatat atataaacag tgtttagata tgattaaata
    11821 taataatatg gatgtgactc caaatatttt aaaacactta acttcagttg aaggaaatga
    11881 agtttctgaa atacttatag aatcagataa tttaggtgtg gttgttgaaa agacgaatac
    11941 caataaattt gagtttgatt acgtaagtgt aactaatgaa aaaaattgta ctttttctgt
    12001 atataaagaa aattcttcta ataatgtaaa aaatggaaag ctagttgtaa gtgctaagaa
    12061 aaaaatagat aagaaagata gtgatttgca agatatgaat ggcgacaata tgataaatgc
    12121 aaataaagat aggatggtaa atgtgataaa aattggagtt cccgataaaa tgtattctaa
    12181 ttttaaagta gatgttgaaa gtggttcagt agaactttat aatattaaag gaggagctac
    12241 tgttgatgtc aatgacggaa ttgctaacat tgttgataac aatgtgacaa atcctttcaa
    12301 tataaatact aatagtggga tttcaggtgt aacagctgaa actatatcat ctgagataaa
    12361 atttagagca aacaatggaa ttgtaagtgt aacagctaca aatataaatg gcaatataag
    12421 tttatttgga agcgataata aaggtacctt tgatggaata tttaaattaa atctgaaaaa
    12481 agaaccatca aatttacact tgaagttagt tggaaatggg ataaataaat tgccagaagg
    12541 ttggtctaaa gattatattt taggaaatgg acaaccaatt attgaagtta aaaataa
//
LOCUS       DACTGW010000105        12490 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.105, whole genome shotgun sequence.
ACCESSION   DACTGW010000105 DACTGW010000000
VERSION     DACTGW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.105"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            172..375
                     /locus_tag="I9573_002224"
     CDS             172..375
                     /locus_tag="I9573_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4796841.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            539..1693
                     /locus_tag="I9573_002225"
     CDS             539..1693
                     /locus_tag="I9573_002225"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAT4796842.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFRVEQENIDVVVHPQSIIHSMVQYADSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1706..2710
                     /gene="rseP"
                     /locus_tag="I9573_002226"
     CDS             1706..2710
                     /gene="rseP"
                     /locus_tag="I9573_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAT4796843.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSNKVVNVTPKNNNGKYEIGIQPQREKDFLASIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2893..3948
                     /gene="ispG"
                     /locus_tag="I9573_002227"
                     /gene_synonym="gcpE"
     CDS             2893..3948
                     /gene="ispG"
                     /locus_tag="I9573_002227"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAT4796844.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSRERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKHGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            4296..5120
                     /locus_tag="I9573_002228"
     CDS             4296..5120
                     /locus_tag="I9573_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796845.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLASTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSIWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(5593..6716)
                     /locus_tag="I9573_002229"
                     /pseudo
     CDS             complement(5593..6716)
                     /locus_tag="I9573_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
     gene            6913..7137
                     /locus_tag="I9573_002230"
     CDS             6913..7137
                     /locus_tag="I9573_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796846.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            7190..7714
                     /locus_tag="I9573_002231"
     CDS             7190..7714
                     /locus_tag="I9573_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4796847.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(8104..9030)
                     /locus_tag="I9573_002232"
     CDS             complement(8104..9030)
                     /locus_tag="I9573_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAT4796848.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9221..11092
                     /locus_tag="I9573_002233"
     CDS             9221..11092
                     /locus_tag="I9573_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAT4796849.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNASCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(11184..12119)
                     /locus_tag="I9573_002234"
     CDS             complement(11184..12119)
                     /locus_tag="I9573_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAT4796850.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
BASE COUNT         4926 a         1304 c         2099 g         4161 t
ORIGIN      
        1 aaatatatat aaagcttaat ttttgaactt atggttttgt tttattttaa catatttata
       61 tttaataaac tataattgaa atctatttag ataaataatt atttatctaa atagatgaaa
      121 attttccaaa atagataaaa tgtgatagaa tatattttgc gaggtgataa catgatttca
      181 tacgctccat tatggaaatt gttaattgac agaaaaataa agaaaatgga atttgtaaat
      241 atatcaggta taagtatttc tgttttaggc agactaggaa atgataaatc tgtttctatg
      301 gatacaatgg agaagatatg tttagcacta gactgtaaaa tagaagatgt tgtggaaata
      361 aaaaagggca tttaataata ctagaatatt ttataatatt ccacaagata caaaaaataa
      421 tagattaatt ttaatctgtg gaataatata tgaaatttct atgaatagta aaaataattg
      481 ttttagtagt gttataatgt tttgacaggg tatatttatt attagaaagg attattatat
      541 gaaaaagata tcaattttag gttctacagg ctctataggt aaacaaactt tggatgtggt
      601 tagagaacat agagataagt ttgagatagt tgcaatatca gctaacagta atattgaatt
      661 attgctagaa caaatagtag agtttaaacc taaatatgta acagtttttg aggaaaataa
      721 ggcattgaaa ttaaaagaaa tgttgcctaa aaatatagaa atagaagttt tagcaggaat
      781 ggaaggatta aaaataatat catcacttga tgaagtggat gtccttttaa ctgctgtagt
      841 aggtatgata ggattagtcc caactctttg tgctataaag aagggaatag acatagcatt
      901 agcgaataaa gaaacgttgg taactgctgg tgaacttgta atgaaagaag cagaaaaata
      961 caatgtaaat attcttcctg ttgatagtga acatagtgct atatttcaat gtctaaatgg
     1021 agaaaataaa aaaaatatag aaaagataat acttacagca tctggtggtc catttagagg
     1081 aaagaaaaag ggagaacttg taaatataac taaaaatgaa gctttgaagc atccaaattg
     1141 gagtatggga agaaaaataa gtattgattc ttcaacgctt atgaataaag gacttgaagt
     1201 gatagaagct agatggttgt ttagggtaga acaagaaaat atagatgtag tagttcaccc
     1261 acaaagcata attcattcaa tggtgcagta tgcagatagc tcaataattg ctcaattagg
     1321 gtgtccagat atgagactac caatacagta tgctcttaca tatccagata gaatggaaag
     1381 tagctttgaa agaatgaatt tttcaaaatt tagtacttta acttttgaag aaccagattt
     1441 agaaactttt ccatgtttaa aattggccta tgagtgttta aaaatgggag gaacttattc
     1501 ttctgtttta aattcagcaa atgaagtttt agtaagtgaa tttttagagg ataaaattgg
     1561 tttttatgac ataccatatt acatagagaa gactttagaa gttcatagta gcataagcga
     1621 accaacatta gaagaaatct tagaaacaga tagatggagt agagcttatg ttgcaaatct
     1681 gataaaaaaa taggaaggtg aatttatgac tataatagcc gcattaatac tatttagtat
     1741 aattgtatta atacatgagt taggacattt tatatttgca aaaagaagtg gtatcaaagt
     1801 aaatgagttt tctataggta tggggcctaa gatatatagt gtaaaaaaag atacagagta
     1861 ttctataaga gcacttccaa ttggtggata tgtcagcatg gaaggtgaag atgaagaaca
     1921 aataagtcca aattcttttg gaaacaagtc tatattacaa agatttagta ctattgtagc
     1981 aggaccaata ttcaatataa tattagcagc aatactttta gttccagtat ttttgtatat
     2041 aggttctcca acgactaaat taggaaagat aatgccagat actcctgcac aggctgtagg
     2101 gcttcaagta ggagataaga taaataaaat taatggtaat tctgttaaga cttgggatga
     2161 agttgcaaat ataataaaca cttcttctgg tggagaatta aaacttagta taactagaga
     2221 tggaagtaat aaagttgtaa atgtaacacc taaaaataac aatggaaagt atgaaatagg
     2281 gattcaacca caaagagaaa aagacttttt ggcgtcaata gtaaatgcat gtaaaactac
     2341 tgtagatatg acaaaacaga tgttaacatt tctaggtcaa atgattacag gacgtgtacc
     2401 aggagggatt ggcaatgctg tagcaggacc agtaggtgtt attggtatgg tatcagatgc
     2461 tgctcgtaca ggcatcataa atgtagtgta tttagcagct gtcataagct taaatttagg
     2521 tatagtaaat ctattaccaa taccagcact tgatggatgg agaatactta tgctattact
     2581 agaagctgtt agaggtggta aaaaactcga cccaaacaaa gaaggcatga taaatgttgt
     2641 tggttttgga gctttaatgt tatttatgct ttttatcaca tataaagata tattaagatt
     2701 atttcaatga tttagttttt tagagatttt aagaaaatag gctatgtagt tttaaataag
     2761 aataaattaa tttttatgta tgaaatttat tcttatttaa ctagatagct ttttaattta
     2821 taatatacat gtaggttata atattttact atttaaagag attcaatata aaataatata
     2881 tagaggtgga atatgagtta cgagagaaga aagacgagag aagtaagtgt aggaactgta
     2941 aaaataggag gagaaaatcc tataagtata caatctatga caaatactga tacaagagat
     3001 gcagatgcta caatagctca aataaagaga ctagaggaag ctggttgtga tatagttaga
     3061 gttgctgttc ctgatattaa agctgctaag aatatagcaa agataaagtc tagtgtaaat
     3121 ataccaatta ttgcagatat acatttcgat tataagctag cccttgaagc tatagaacaa
     3181 ggtgtggatg gaataagaat aaatcctggt aatattggaa gtagagagag agttaagatg
     3241 gtagttgaga aatgtaagga aagaaattta aaaattagaa ttggagtcaa tggaggttct
     3301 ttagagaaag aacttttaaa aaagcatggc tctgcaactg ctgaagcatt agttgaaagt
     3361 gcaatgggac atattaagat tcttgaagac ttagattttc ataatatagt tatatctcta
     3421 aaatcatccg atatttataa aactgttgat gcatatgaat taatatcaaa aaaagttgat
     3481 tatcctcttc atattggaat tacagaatct ggaagtgttc acaaaggaac aataaagtct
     3541 tctatagggg taggagcact tcttcttaaa ggaattggag atactgttag aatatcttta
     3601 acaggcgacc ctgttgaaga ggttattgtt ggtaaacaga ttttaagaag tttgggactt
     3661 ttaaatgata agattaaagt tatatcatgc cctacatgtg gtagatgtaa tatagactta
     3721 attagtgttg taaatgaggt tgaagaaaag attggtagta tggaaaaaaa tatcactgtt
     3781 gcaattatgg gctgtgctgt aaatggacct ggtgaagcca gagaagcaga tataggtatt
     3841 gctgggggta aaggtgaagg tcttttattt aaaaagggtg aaatagttag aatgattgat
     3901 ggtaacaaat tagttgatga gttgctagaa gagattgaaa aattatagta tacaattcat
     3961 atatatatta attgactaga tttttaaata cttttgtaat aagtttttat ttacagtatt
     4021 taattatcta gtcatttttt attttataaa cttcattaaa tgtaagaaac ttatattcta
     4081 atttgactat atatgtatga gttaaatcat attgtaaatt tataaaatta aaaaaagatt
     4141 taatatagtg aaaatttcca tcaatagtat tgaaacttga tagtaactat gataggatat
     4201 agttactaga acagttggta aattatgtta acatagattt taatgttttg gtaaattgtt
     4261 ttattataag ttaatagaaa aagaggtgaa gaactgtgag atataaaaaa atatctagct
     4321 tacctatttt agttgttgct ttgctgatta ctggaacgac aacaacattt tctcttagta
     4381 cgaccaattc agaaaaaaca aatgtatact tagcaagtac ggtaagtact gtcacaaaga
     4441 aagtatccga tattcaaagt agtaaaggtc ttgtttataa tgagaatatt ccattattta
     4501 taaataatag caaaatacag tatgatgata ctccatatca ttggcctagc aatgttataa
     4561 gcttaacaaa tagcagtgaa aaggcaatta tggattatga gattacatgc ttagcatatg
     4621 ataaaaatgg aaaaccactt gagctatatt gggatgctca aaatgtggca gcagatggcg
     4681 aagtaggaag tgttggattt agcccagctg gtgtagacta tggtattgtt acaggcatat
     4741 ctcctgtgtc tccaaaatca tattcacata cttatagaaa aatgcaacaa tcaccaccac
     4801 aggatataat aagtatgttt gaaaaacaac aaggcaaggc atgggtagaa aattggttga
     4861 aagaatggaa acaaatggag aaagaatatg caaagcaaaa tgcaatagca ccaggtaaga
     4921 atcaaaatga tgcctttcta ttgtttgata aatggaaaca atcaactgga gagcatggtg
     4981 taaagtacat tatatcttgt gtaaaacaag taacatttaa tgatggtagt atatggaaaa
     5041 attcagcata tgagaattgg ttgaaaagtt ttcaagggaa ggaagtttca aatagtgtat
     5101 tagaaaatta ctataagtaa tgtttaatga tagcaaaatg agtacgaatt taattaatgt
     5161 taatttaatt aaataaggac tattttgttt aaaaatatca gtgttacaag gaaattttta
     5221 ttgtgacaaa atggtatata ttcaaaataa aaaagcatgt gtcaagtttt gatacatgct
     5281 tttttatttt gaatatatgt tttctataaa tttatgattt tttagttatg taattttata
     5341 tgtgaatttt tagattttta tatagatagt gaaataatgt ggaagtttta tgttttaaaa
     5401 attttagtta aaacatttat taaaaaagat aatagaaatg ttttaactaa aatagttaga
     5461 attgaaatgt tatacagata gtataagtac tatcaaatta tataagattg cttgaagtta
     5521 aataatttat ttaaatagaa aacagctgat ttaatatcag ctgtatatga ataaaatttt
     5581 gaattgtagt gtttagttat atggatataa ttcataataa gttagcaata aatcaaccat
     5641 tttaccataa ctctgagtac cttcagtaac gccattagct tttaagtaag aattgttaaa
     5701 ctcattagaa atttcattaa ttttaccttc atatttttgc caaaattcac tttcattttt
     5761 taaatctcta cggacagaat ctgaaatgcc agcagaaata tcaacatatg cattataatc
     5821 aaccttactt aaagcagaag cagtataatt taaagctaaa atatatccag aatagttaaa
     5881 atcaatatgg gggtgtttta tagaagtaag atacgctata aaattagcct catcttcact
     5941 agcaaatccc ctttgatgtg ctatttcatg ttcaactgta caaggaatat ataagtcagg
     6001 tattgctata tttacatttg cctcacctgt aaaaggaaag tatataccag ttattcctgt
     6061 atagcacatt agatttgatg aaataatata cttaggctta gaataactac cactaacact
     6121 aggtaatata tccaagatat tatcgtatcc tagttgagct ctatttatga caccattata
     6181 atctgtatta gattttacaa taccattttt atcttgtaat gtgagttttc tagtttcatt
     6241 tgctttaatt acaaggaatt tatataattt agtaagctct ttaacactat attgttttga
     6301 ttggatagag gtattgtttt ttaaattata gttatttata agtgtggttt ctagtggaat
     6361 cctattatag tttattcccc aaagcactac aaaaagaaag tagactattg atacaatgga
     6421 taatatattt aaaatggaat tttttaaaaa tatgtttaat tttttttctt attaaaaatt
     6481 gtagataaag tacaacataa aaataagaag atagatataa ctattatgta cattgtaatc
     6541 tcatagagag aaaatgggaa aattccagaa actttactca atatttgtac aattatttta
     6601 tctatgaatt gggagtaata tttttcaaca atatttggaa ctttgctggc tataaaattt
     6661 aaaagtaaag caataggaaa gagaattaaa gaaaaatatt ttgttttttt gctcatattt
     6721 cacctcacat acattagttt atataaacat attatatatc aattgaagaa agattgtaaa
     6781 ataagtataa atatatctat ttataacttt atactaaata aatgataagt tataatatat
     6841 taatatgaat aagcagtata tatacaacta ataataagaa aaatatcaat tatataagga
     6901 gtagataaaa atatgataat aaaacttact aaagacaatt ttaattttag atttaaacaa
     6961 tatgaaggta tgttttttca tgctgaagta gttgaaggta tggaatccat tagatataaa
     7021 ataactaagg ataaagaagg tacagaatta aaagaatttg gaagtacaca tataaaagat
     7081 aatatgatat atgtacctca ggtagaagca tacatagatt tgaacagtat aaaataaaga
     7141 tgtaaaattt taattaaaaa taaagattat gtttgagggg gaatccaata tgagtaagcc
     7201 tatagatata acaataagac ctataagaag agaagatgca caagctttta atgaaattag
     7261 aagaacatac agtgttatga gaaatacttt ggcattaatg agtgacagag tcgataaaac
     7321 tgaagctatg ttagcttctt tgggagaaaa tgattatatg tttgtagctg aaactaataa
     7381 taatggagaa aaagatgtaa taggttttgc tgggttacat gtaaattctt ctccacgatt
     7441 gagacatagt gcagaaatag gaataagtgt tgcagagaat tatcagggaa gaggagtagg
     7501 taagaagctt atggaacaac ttttagatat agcagataat tggattatgt taactagagt
     7561 aggattagaa gtaattgtag ataatgaaaa gggattaaat ttatataaaa aactaggttt
     7621 tgaaatagag ggaactaaaa aatatgctgt aatcagagat ggaaaatttg aagatgttta
     7681 tattatggga agatataata aaaatttaat ttaaagaagc taatagagaa gtaaaataga
     7741 gtgtctttaa taaaaaattt acattaaaaa taatctattt tacttctcta ttgtattaaa
     7801 aaacttattt ttaaatgttt acaaaaacat tctatataca aataaaatta ttagcaatat
     7861 caacgctatt ttaaaagcta aactagcaac agttttaatg atttttacag aaacacctat
     7921 agcaactata gcaaatacta ttttaaagag taaatccata tccatattta agcctcctat
     7981 tctataatac atatagatat tattgacaaa tatctaggaa aaaattccat caatatacta
     8041 caataaaatg tttatatttg taatttattg aatatgttat tatataatac ataaaaaaat
     8101 aaatcaagat aagagtaaaa tacttataaa aggtatagat ataactgaaa atagtgttgt
     8161 aaaaaacaca cattgtgaag ctagtgttat attggagtca tattgattag ccattatagc
     8221 agtatttgca gcagcaggca ttgaagaaat tactactggt atagctaaaa tcatcttatc
     8281 atttatccat ccttttagaa taaaatacac aatcacagga agtaccaata atcttataaa
     8341 agtgactata tataattttt tgttgacaaa acaatcgaga gctgaagaat tagcaagtaa
     8401 gcttccaatg ataatcatgg atattggtgt agttatactt cccaacatct caagtggctc
     8461 gtttataaat tggggtaacc taagtcctgt tacaaataaa aaaagtccaa taacaatagc
     8521 tattgtagca gggcttataa gtgattttat tgaaaaacct ctacttgtac tgcctttaga
     8581 taaaagataa attcctaaag tgaaagaaaa taagttaaaa ggcaaattga atattgcagt
     8641 atagaatatt gcttcatgac ctaaaactgc ttcaattaca ggataaccca taaaagcaac
     8701 atttgaaaaa acaactatat actgatatat tcccaaatct tttttatcat tacattttaa
     8761 aatgtatttc aataaaaatg caattataat tgaaactata tacataatca aagatgtaaa
     8821 taataacaat agtatttttt gtatcatttt actatcaaag ttaatttgca tggagctaat
     8881 tatcattgat ggtaaaaaaa ctgtcattgt caaagttgaa agcttagatg tgcagtggtc
     8941 atctaataag ttgaattttc tgacaaaata tccaactaaa ataattataa ataaaactgc
     9001 aacttggata aaaatattgc tgaaattcat aaaatgtccc cccctatata tacattatat
     9061 accaattaca taataaaatc taatgattat aaaatttaaa atttaatgtt acaatatagt
     9121 gacaaaaggt aaaaattcaa tattatagca ttaattatag attataatgt ataataatgt
     9181 ttgagagaat atattttata aaatgggtag gtagcgaata atgaaaaata caaaattttc
     9241 tataaaaaaa ataaaaaata tatctaatga cccgaaagtc gtacatacta agggtgccct
     9301 ccttgaaaag atgggtaaga tggaaagcgc aatagagttg tataagagtg ctgcattgga
     9361 taattacgta aaatctcaat atactttagg aaatatatat gaaagtaaaa aacaatttaa
     9421 agaagcagaa aagtggtatt caatggcata taaaagtgga agtgaagatg ctgcttttga
     9481 tttaggtaat atgtactata agttagatgg ctatgaatat gctatatatt ggtatgaaaa
     9541 gatagcaaat ttaggttatt tacaagcaca aaataattta ggtgtatgcc attttaaaat
     9601 gaaagatttt gtaaggtgtg aaaaatggtt aaaaacagca gcagacaaga atttaggtaa
     9661 agcttgtttt aatttagggg tattatatac ttttttggag aaagatgatg aagcatatga
     9721 atactataaa aaaggttctg tgcttttaga tgatgatgca aaatataatt tagcaatatt
     9781 aaatggaaaa aagaaagata ataaatcaac agtaagttta tataaacagt tgtacaagtc
     9841 aggtcatatg aaaggttgct ttaacttggg tatgattatg gaaataaatg ataatttaga
     9901 agatgcagag aagtattata aaaagagtgc tgatagagat gatgtgaagt ctgagtatag
     9961 gcttgcatat gtatatgata gaaaagaaga gctgggagat gcaatttatt attatgagaa
    10021 agcaatagaa aaaaatcata cactttctaa gtatagactt gctaatttat ataatagaga
    10081 aaatgagata gagaaggcaa aaacttatta taaaatggct gctgaagatg atataacaga
    10141 agcaaaaaat aatctggcag gtatatattt tgaagaaaaa gattatgaaa atgctattaa
    10201 atattatgag gatgctatag ctgttggatg caaaagttcc cttgaaaatc ttggggattt
    10261 atattatcag aatcaagata tagaaaaagc gatttcgtat tattcgagga ttccaaataa
    10321 tgcatcttgt caaatcaaat taggaaatat ctatgaagat ttaaataata ttgaagaagc
    10381 cataagttgg tataagaaag catctgaaaa tggagatacg aggtctagtt atagattggg
    10441 gtgtatatat gagagcttag gaaatacaaa aaatgctaga aagtattttg aaatggcaag
    10501 tagcaaaaat catatgaatg ctcgtataca tctaggtaga atttatttta gagaaggtaa
    10561 attggaagag tctaaaatga tgtttgatac acctgccaat gaaaataatg tgtacgctca
    10621 acatatggta ggattaattt atgatatgtt ctataaagat tatgtaaatt ctaaattttg
    10681 gtatgaaaag gctagagcac aaggttgtgt tgaatcaata tataatttag gccaaattta
    10741 tttaaaatta aatgatgacg cagaagctga gaaatattat aaagaaggta ttaaatatgg
    10801 aagtaagaag tgtgagtata tgcttgcagg tctttattat aaaaagagcc tagatatgta
    10861 catttcttta gcaaatgaag agtacgataa ttgtgaagag atagtagaag gctttcccaa
    10921 tttaaatata gactttgatg aagtattaat tcctccattt aagttgcaag aagaggtaat
    10981 agatgaagaa gagtatgtac caatttatat actaaatata aaagaaagtt tagattcaat
    11041 gctagaaggg cttgaaactg aaatgattat agatgaagaa catgatagtt aaaaagtatt
    11101 ataagattat atagatttaa atatataaaa gccattagga taaatgtaaa tcaattaatt
    11161 ctaatggctt ttttattaat attttataat agcttatttc ttgacatgaa aaatgtaatc
    11221 actatacata ttatagaggt aataagaagt attatccaaa atccgtttgg gttattagaa
    11281 aatggcatgt tgatgacatt cataccaaag aatcctgata ttattgtagg tattgagaaa
    11341 attagtgtaa ctactgttaa aatttgcata acattattct gattattact tataattgca
    11401 ctgaaagcat ccataattct actcaatata tctccatata ttttagccat atcaagagcc
    11461 tgtctatttt caactataac atcttctaat aggtcttcat catctggata cttctttata
    11521 ccagcagttc taaccatttt gtttaaaaca agttcattag attttagtga ggtagagaag
    11581 tataccaaag atttttctaa ttccaataat tgaaccaatt ctttgttttt cattgatttg
    11641 taaatttctc gctctattat gattgtcatt ttatttattt ttcttaagta atgcaagtaa
    11701 taagctgcat ttttgtgaag aatttgaagc aaaaagcgag tctttttaaa cgtaaaaaaa
    11761 tctttaatat gaccaactat aaagtcattt aaaatcttag tctgtgcatc acaaactgtt
    11821 acaatagctt cttcagttag tataatacct aagggtattg tagtataatg agaagaatta
    11881 gagtcacttt cgtctactgg tatatctata agtataagag tatgattgtc ttcaacatct
    11941 attcttgagc gttcttcttc atctagtgca gattctatgc tttctacatc tatatttaat
    12001 aaattagaaa tttcgttgat ttcactatga ttaggttcaa caagatttat ccaacagcca
    12061 tcttcaaaaa aactaagttt ctctaatttt gaatctatag ttttgtaata tcttatcatg
    12121 gcaacacatc ctttattcag ttttatataa atctcatcat ttaatagtat ctattaatta
    12181 actaatttat ataactgaat atatgtatta gttttgattt tagatattat taagtgatga
    12241 aaatgtaaaa aattctctcc gcaacggact agtatccatt gcccatcact ctccttattt
    12301 attattttat atatagctat ttatgaaatg cgtattttaa gcactcttta atataatacc
    12361 ttttccaggt atataaatca agttttgatt tgattaaata atatttttac cataatttac
    12421 acataaagta tataagttat atttaatttg agtaaagaat atatatattc tgagtgaaat
    12481 tattaaatat
//
LOCUS       DACTGW010000106        12327 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.106, whole genome shotgun sequence.
ACCESSION   DACTGW010000106 DACTGW010000000
VERSION     DACTGW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.106"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            228..1157
                     /gene="glsA"
                     /locus_tag="I9573_002235"
     CDS             228..1157
                     /gene="glsA"
                     /locus_tag="I9573_002235"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAT4796851.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1255..1818
                     /locus_tag="I9573_002236"
     CDS             1255..1818
                     /locus_tag="I9573_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4796852.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2306..3142
                     /locus_tag="I9573_002237"
     CDS             2306..3142
                     /locus_tag="I9573_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAT4796853.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHIGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            3394..4389
                     /locus_tag="I9573_002238"
     CDS             3394..4389
                     /locus_tag="I9573_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAT4796854.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRNKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(4646..5050)
                     /locus_tag="I9573_002239"
     CDS             complement(4646..5050)
                     /locus_tag="I9573_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAT4796855.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     FLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            6050..6964
                     /gene="queG"
                     /locus_tag="I9573_002240"
     CDS             6050..6964
                     /gene="queG"
                     /locus_tag="I9573_002240"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAT4796856.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFVDNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEMPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            7070..9307
                     /locus_tag="I9573_002241"
     CDS             7070..9307
                     /locus_tag="I9573_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4796857.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNRLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDKSDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNESSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            9900..10556
                     /gene="nth"
                     /locus_tag="I9573_002242"
     CDS             9900..10556
                     /gene="nth"
                     /locus_tag="I9573_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAT4796858.1"
                     /translation="MKSESANKKDVNKILDELEKLYPDAKCELNYGTAFELLIATILS
                     AQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLC
                     ELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPN
                     PQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK
                     "
     gene            10750..11220
                     /gene="trmL"
                     /locus_tag="I9573_002243"
     CDS             10750..11220
                     /gene="trmL"
                     /locus_tag="I9573_002243"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAT4796859.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            11256..12104
                     /locus_tag="I9573_002244"
     CDS             11256..12104
                     /locus_tag="I9573_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAT4796860.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVDEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
BASE COUNT         5058 a         1128 c         2293 g         3848 t
ORIGIN      
        1 gttaggttag aaatttttga cgttataaga atttaaatta ctggattatt agattactaa
       61 attactagat tattaaatta ttaagctatt taaactgttt aaattgtttg aaaaatgaat
      121 tgattcttgt agaaaatagt tttaaagtat aatttttttt aacactgttt tattatagta
      181 caaacttgtg ctaaaataga atatataaca taatataaga ggtgcatatg aatacgttag
      241 atgagacatt attaaaagaa attataagtt ctaataagaa gtatactaat tatgggcaag
      301 tcgctagtta cataccagaa ttaaaaaacg ctagaagaaa tgatttagga atttgtatca
      361 tagatagcga aaataatctt tatagtgctg gaaactgtag cacaaagttt actatacaaa
      421 gtatttcaaa accaattgtg cttgctatgg cacttatgga taatgattgg gaagacgttt
      481 tttcaaacgt tggaatggag ccaagtgggg acccatttaa ttcaataatg aaacttgaaa
      541 ttaatgacac aaaaaaaccc tgtaatccaa tgataaatgc aggggctata gtaactactt
      601 cccttataaa tgggagttgt ttagaagaaa aagaagaaag aatgcttagt ttctttagaa
      661 agttagcaaa aaatgataat ataggaataa actatgatgt ctataagtct gaaaaaatga
      721 ctggagatag aaacagagct atggcttact tattgaaaag tgatggattt atcagaggga
      781 atgtagaaga tgttctagac ctttatttta aacaatgctc aattgagata gattctgtag
      841 atttagcgag aattggaata aatcttgcaa attatggagt agatatagaa aatggtgagc
      901 atttaatgag tgaaatggtt tctaggattg tcaagacatt tatgatgact tgtggaatgt
      961 atgatgcttc tggggaattt gctattaaag ttggcattcc tgcaaaatct ggagttgggg
     1021 gtggtataat ggcttctgtg ccgggacgca tggggattgg tgtttatgga cctgcgcttg
     1081 acaaaaaagg taacagcgtc gcaggagtta aagttttaga ggagttgtca aataaactca
     1141 aattaaatat tttttaaaat attaagaaag gatttttttg acaacaacat ataatttaat
     1201 atataattaa attgtaaaga aatatgaatt aaataaattt tttaggaggg gtcaatgaaa
     1261 gaggtattgg tattacgaga tttggagtgc ataaaggcta ttgcgcatcc acgtagaata
     1321 gatattctaa aagcatttga caaatcgcca ttgtcagcaa aacaattatc tcaattattg
     1381 gaagaacctc atgcaaaaat aaactaccat ataaaaatgt tatacaaagt tggtatttta
     1441 gaattagttg aggaaaaaat aaagtcgggg attgtggaaa agtactatta tccaagtgca
     1501 aaaaatgtgg ttatagggaa taggatactt aatttttctt tagacaatgg ggaagaaaaa
     1561 gaagaattgt acatatcaaa atttgaaaat atgagtgaag ttttttataa ggcagcagag
     1621 gaagatgttt tagaaaatga aaatatagtt gattatcatg atatttctct tactcatgat
     1681 gaattagttg aacttagtga cactatgaag tcaaagatag atgaaatttt aaataaaaga
     1741 caacacaatg ttgaaggttc taagtatgat atagctatgg ttatagttcc tactcttgag
     1801 gaggaatgtc ctagttagag aattaattat ggattaatta aagatataat attagagatt
     1861 gaacatcaaa atttacttaa taatcaaaaa atagtttttg gtatgggtaa aacaatgatt
     1921 tttaatctct atttttttgt tttcttatac ctattgtatt tgtaaataat atgtatagtg
     1981 taagatgtat acactatctg agaagtgctt agataatata caaagtatgt aagattataa
     2041 tatttgaaat tgattaaaat gggtataatt aaagtgtaaa tattaaaatt aaaaattaat
     2101 ataaattaaa tcaatgctag aaatatgtta aaagaggaat gagattagtt gggatagaac
     2161 atcccttaga gcttatgaat ttttagtgaa aaagtactat caaataggaa tctccatctt
     2221 catatgttgg ctaaatatag ttaaggtgga gtagttcaaa attaaaattg agtacaagtc
     2281 gtagtacaaa taaaaggaga ttaatatgat taaattagga acacatcttt caatagcaaa
     2341 aggatttgct aatgcggctg agacagctgt atatattggt gcaaatacat ttcaattttt
     2401 tagtagaaat ccaagaggag gaaatgctaa agaatttgat gaaaaagata taattaaatt
     2461 tcaagaaatt agaaaagaaa ataattttgc accactgctt gcacatgcac catatactat
     2521 gaatctgggt ggtacaaagg atgatgtata cgaatttgca acaagggtga tagatgagga
     2581 tataagaagg atggactcat taggaattga atacatgtgt tttcatcctg gaagtcatat
     2641 aggtggtggt gtagattttg gtatagatag aatagtaaaa ggattgaaca atgccataaa
     2701 gggtgatgaa aatataacta tacttcttga aactatgtcg ggaaagggaa ctgaaatagg
     2761 ttttaaattt gagcaattaa aaagtataat agacaaaatt gagcataaag aaaaaatagg
     2821 tgtgtgtctt gatacatgtc atattttttc agccggttat gatatagtga atgatttaga
     2881 tggggttttg gaagaatttg ataaggttat aggcatagaa agattgaaaa ctgtccatct
     2941 aaatgatagt atgatgccat ttggagacaa aaaagacaga catgctccta taggggaagg
     3001 aaagatagga ttaaaagcat taattgattt tatggaacat cctagtctaa aacatttacc
     3061 atttttctta gaaactcctt ttgatgatga aggtcacaag agagagctta aaatgataaa
     3121 agaaatacta tctagtaaat aaacctttat taaaaatatg tgaaatactc cgccctcaac
     3181 ataattaagg tggagataca atcatgaaat gtgataattt aaattaatgg ataggaataa
     3241 caaaaattgt atacaaatta actgggtata aagtatgatt atattatgaa tagtatgtaa
     3301 atatattgca aagattctca taatttgttg tataattaaa ggtaacatat ttatattata
     3361 taaaaaatta ttaaaagtta ggtggggaaa gatatgagaa atttaggcaa tactaacatg
     3421 aaaataaaga gagttggctt tgggggcatt ccaatacaaa gaattactca agatgatact
     3481 aacttagtta taaatgagtt agaaaaacaa ggaataaact ttattgacag tgcaagaggg
     3541 tatacaataa gtgaggaagc aatcggaatt gccattgaag gaaaaagaaa taaatttttc
     3601 ttagctacta aaagtatgtc cagagattat gattcaatga agagagatgt tgaaataagt
     3661 ttaaataatt ttaaaactga ttttatagat ttatatcaat tccataatgt taaagaagag
     3721 gagtatgata atctttttaa agataaaatg gcttatagtg cccttttaga ggcaaaggag
     3781 caaggtaaga taaaacatat tggtataact agtcacaatt taaatactat agaaaaagct
     3841 atagaagatg gaaaatttga cactatacaa ttcccttaca atatagttga aggacaagca
     3901 gatgaagtgt ttaaaaaagc tcatgaaaaa ggaattggga taatagtaat gaaaccttta
     3961 gctggaggtg ctttagacaa tgcaacactg gctattaaat atatactatc taaagattac
     4021 atagatgtag ttataccagg tatggagagt gttgaacagg ttagacaaaa tgtcgctgta
     4081 cttgaaaatc ttgttctaga tgaaaaagat aataaggaaa ttgaagaaat aagaaattct
     4141 ttaggtaaaa aattctgtag aagatgtgaa tattgtatgc cttgtgctgt aggaataaat
     4201 ataccattga gttttttatg tgaaggttat tatactagat atggattaaa agagtgggct
     4261 aaagaaaaat atgaggttat ggatgttaaa cctactgagt gtattgactg tgggctttgc
     4321 gagagtagat gtccatatga acttcctata agagaaatgc ttaagactgt tgttgaaaag
     4381 ctaggataga ttttatttat tgaaatcggt gtattaaatt tactccgatt tctttttatt
     4441 agatgtggtc ttatatacaa atattaaatt tacatagtta tagctattat ataaatttaa
     4501 tatttgttta atacttatta aatatttgct taatgctttt taaaaacatt tgtttgagta
     4561 tagacataac acaattatta ttgttgtatt agtatagttt caaatactgg agtatcaata
     4621 taatgtcgaa taacacataa aatctttatt tttcaaaaat attataggat atatcgtatt
     4681 tagatactgg ataaagttca atttcaaccc tagggttatc tttatctata aaatcaaata
     4741 aaaatactgg cttaagttgt ttatcatttt ttattattcc acttttttca ataccatcac
     4801 aaatactttt tggataatta tgtaaatccc ccattctctt atttggaaag aataatttca
     4861 agactgttat tagattttct tcaacagttt ccccttgata ctgttggttt ataaaacctg
     4921 caatcatgtt ttcgtatgca agatacttag aatattttcc ctttgaaggc atccatgctt
     4981 gaccatttat gttatgaagc ttaaaattag atttactaat aggtctacct ggaattgtta
     5041 ttttaatcaa atttttcacc atccttatac agtatttatt ataatctgta tatgatttaa
     5101 aagcaaattg ttatttaaag tgtttttaca gggcttgttt gtaaaatttt ccactttaga
     5161 tgtagtaata taagataagt attttgctta caaattttaa aatttgatat atgatggaca
     5221 tattcatatt agactattat aagaccttca taatattaat aagtataggt ttagtaaaga
     5281 gagtaaaaag aaatccatat aagccaagta aagacttaat aaatggcaat gtaaatggga
     5341 tattaattat aagaaaaaat caatcttttt gcattaacta taggaagaat gtcaaaatat
     5401 aactaatgat atttgaagtt ttagagttta tattaaaagg taagaaggtt aagtgggtaa
     5461 aaaaggttga atagattaaa aaggctaagt atctaaaaga gttaagtagg taaaaaagtt
     5521 aaataagtaa atgaaaaaag ctaagtatct aaatgagtta gtagataaaa aaattaaata
     5581 gataaaaaga ttaagtatct aaaagcgtta gtagataaaa aaattaaata gataaaaaga
     5641 ttaagtatct aaaagcgtta gtagataaaa aaattaaata aataaaaaag actaagtatt
     5701 caaaagagtt agtaggtaaa ataattaaat agataaaaag actaagtatt taaaggagtt
     5761 aagcagataa aaaagttaaa taagtaaatg aaaaagacga agtatctaaa agagttagta
     5821 aataaaaaag ttaaataagt aaataaaaaa gatgaagtat ctaaaagagt tagtaggtaa
     5881 aaaactaaat aggtaaaaag acgaagtatc taaaagagtt agtaggtaaa aaagttaaat
     5941 aaataaaaaa gacgaagtat ttaaaagagt tagtagctag aaaaggttaa aaagactaaa
     6001 aaaattaaat aaatttaaaa agtcaattag attaaaaagg aataatttta tggataataa
     6061 ggaattaaaa gcattttgta atttaatagg attaaactgt gtaggagtag caggggtaga
     6121 taagtatgat aatttagaaa agatacttaa ggacagacaa caaaaaggtt atttaactgg
     6181 tatggaagaa ccaataattg agaacagaat taacccaaga catataatga aggatgctaa
     6241 ctctataata gtctgtgcat ttccatatca tataaataga caagaaaaga ataaaaattc
     6301 aaatttatca aaatattgtc atggaaagga ttatcacatt gtagtaaagg actttttaca
     6361 acaaatttgt gattatattt ctaaaacagt aacagatttt aaatacaagt tatttgttga
     6421 taatggacca ttagttgaca ggtatttagc ttatttatca ggaataggat attttggaat
     6481 aaataataat atcataactg atgaatatgg ttcttacgta tttatagggt atatagtaaa
     6541 taattataaa tttgaagaag ataaaccact agaaaaaagt tgttttaaat gtggtaagtg
     6601 tgtaaaatat tgtccgggaa atgctatatt gggaaattat gatatgaacc ccaaaaaatg
     6661 tttatcatat ataactcaaa aaaaaggaga tttgtcagag aaagaaaaag ttgttttaaa
     6721 gaatggcaaa aaggtatttg gatgcgatat ttgtcaagaa gtatgtccac ataatgttga
     6781 aatgccaaca actcatattt tggaatttaa agaaaatata atagattatt tggattatga
     6841 tgagatacaa aatatttcaa ataaagaatt taaaaggaga tatggagata gagcttttag
     6901 ctggagaggg agaaatataa taaagagaaa tatggaaata attttagata agtatgatga
     6961 ctaataataa gagtctgttt catatacttt aatgtgtatt tactttaaca cttgttgtgt
     7021 aaaagtcata ttatatcatt aaagttaaat atggggaggt gggggaatat tgaaatataa
     7081 taatacatct gaaataaatc cactagatta tatattgggt caagaaagag ctgtagaagc
     7141 tatggaatta gggttgaaaa taaataatcc agcgtacaac atatatatag ctggtgaacc
     7201 tggaactggt aaaagtactt atgccttaaa ggttttaaat gaatatgcat ccaaaaaaaa
     7261 tacacataaa gattggtgtt atatatataa ttttgaaaac cctagagagc ctattatagt
     7321 tgaattagaa aaggggtttg gaagagaact taagaaagat atggagaaat taattgaaag
     7381 tcttttagaa gattttaaaa atgcgtttga aagtgaaaat tttgaaatag aaaaaaatag
     7441 acttctagat gaatatgaaa tagaaaaaga tatgcttttg aagcaaatta agaaatatgg
     7501 ggaagaaaaa ggatttaaat taaaacagag taaaatggga atagttttta tccctttgaa
     7561 agaagaggaa gatgaaagtg ataagtctga tgaagagttt tacaaagcta aaagagaact
     7621 agaaaatatg gcgatacagg tcgtatataa aattagaaac ttagaagaaa tagctgaaaa
     7681 agcagtgtta gagcttgaag aagagatagc taaacttgta gtagaacctc atatcaagag
     7741 attgtgtgaa aaatatgaaa attatgataa gattcaaact tatctggaaa atataaagaa
     7801 agatataata gaatatatgt atttgttcta tttagatgaa gaggagttaa aagataagta
     7861 tgataaagag cattttatta aatacaaaat taacctattt gtagacaatg aaagctcaaa
     7921 gaataaagaa tcagcacctg ttgtagtaga gataaaccca agtccagcaa atctatttgg
     7981 aaaggcagag tatgattatg caaatggaaa tataaagact gatttcacaa aactattatc
     8041 aggagcattt catagggcta atggtggtta tttagtactt tatgcagacc aattactaaa
     8101 atatacaatg tcttgggaaa ttcttaaaaa gactttgcag actaaaaaag taatattgga
     8161 aacacaaaca gcaattaaac cagaaaatat gccattggat gttaaattag tactgattgg
     8221 aagccactat atatatgata ttttatatag atatgatgaa gattttgaaa aatattttaa
     8281 agtatttgta gactttgata gtgaaatgga taaaaatgaa gataatgaag aaggaatagc
     8341 aagatttata gcataccaat gtgataagaa taatttaagg cattttacta aatcagcagt
     8401 tgaagaggtt attaaattta gcactagatt atctggtgat ttagaaaaac tatcaactaa
     8461 gtttaataaa ataatggaag ttgtaataga aggaagtgct tatgcagagt ttagaaattc
     8521 ggagtataca gagcagtgtg atgttaaaaa agcaataagt gaaaagagaa aaagaatcaa
     8581 tagagtggaa acacatatgg atgaatcaat tgaaaatgga tttactctaa tagaaacaga
     8641 gggaagaaaa gtaggagtga taaatggtct gtcagtctta agtacaggag aatactcttt
     8701 tggtagagct tcaaggatta cagcgaccac tagcccagga agtaaaggca tagtaaatat
     8761 agaaagagaa gtaaatatga gtggctctat acataacaaa ggagtgctta tattaggtgg
     8821 ctatctttct gagaattttg cacaggattt actattgtca ctaaacgcat atgtttgttt
     8881 tgaacaaaat tatggtggaa tagatggaga tagtgcatct tctgcagaat tatatgtact
     8941 attatcatct cttagtggag tatcaattaa acaaaatatt gctgttacag gttctataaa
     9001 tcaaaagggt gatatacaag tagttggtgg tataagtgaa aagatagaag gattttattc
     9061 tatatgtaaa actaaagggt taaatggtga gcaaggcgtc ataattccta gaaaaaatca
     9121 aagaaatctc gttttatcag atgaagttaa tgatgcagtg cgagatggaa aatttaagat
     9181 atatactgtt gaaagagtag aagaggctat agagatattg acagatgtta aatttgaaga
     9241 aataaagaaa ttggtaaaag aaaaattaat atctttcagc aagatacaaa ctgtgtcaaa
     9301 agaataagtt tattatagaa tttaatgggt atttatgtat aataaaaagc aagaacttta
     9361 ataggttttt gctttttttg taaatatata taaattaaat tcttattttg tatgttttat
     9421 atattgatgt ttattttttg ttgatatgac taaactattt ataaatagtg ttttataaat
     9481 tggtatttta aagggggata attatgaaaa atgtaaatct aaaagagata aatgaaaaag
     9541 atgtaattgt ggaagaagat ataaatctaa aaggaatgga taagaaagat gtaattgaga
     9601 aggaagaagt aaataaaaaa agtatagata agaaagatat aattgagaaa gaagagatag
     9661 ataaaaaaaa tgtaaagaag gttgaggttc aaaaagaaat agataaaatg attagtaaaa
     9721 aagatgagat aattgatgag aaagatatag agaattttag gtatattgaa gaagataaaa
     9781 aaagtatcaa agtaagtaaa aaaagtacta aaaaaggtga aacggagaaa aaaacaaaga
     9841 aaagtagttc taataaggct acacaaaaac aaagcaaaga agttaaaagt aagtctaaaa
     9901 ttaaaagtga atcagccaat aaaaaagatg taaataagat tttagatgag ttggaaaaat
     9961 tgtacccaga tgctaaatgt gagttaaatt atgggacagc ctttgaactt ttaatagcaa
    10021 ctattttatc agcacaatgt actgatgtaa gagtgaataa agttacttca gagttgttta
    10081 aaaagtacaa cacagctaga gactttgcta atttgagcat tgaagaaatt agtaaagaga
    10141 taaagagttg tggtctttac aaaagcaagt ctcaaaaaat aaaggataca tctgaacagc
    10201 tatgtgaatt atatgatggt gaagtacctg atagcttaga aaagcttata aaattacctg
    10261 gtgttggaag aaaaacggca ggggttgtac ttagtaacgc ttttaatcat ccagcaatag
    10321 ctgtagatac tcatgtattt agagttagca atagaatagg aatagtggac gagccaaatc
    10381 ctcaaaaaac agaatttgca cttatggaag ctataccaaa agaaagatgg tcacactcac
    10441 atcatgtatt aatttttcat ggaagaagaa tgtgtaaagc tagaaatcca gaatgtgcaa
    10501 gttgcccaat aaaagaagat tgtaattatt ataaagaact taatgaaacg aaataacttt
    10561 tagaaagtaa gttgtacaag agggattttc tgatataata atacttgtga aaatgttaga
    10621 taaaaaacta ttatatacat gaaataaaaa ttattattaa cattatttta tataatagta
    10681 taatattatt gttattctgt ttataaaata aaatttgata aataaaagcg atacttaggg
    10741 aggattaaaa tgtcattaaa tatagtatta gtagaaccag aaatacctca aaatacagga
    10801 aatataataa gaacctgtgc agcaacaggt tcaactttac atttagtaaa accactgggt
    10861 ttttcattgg atgataaaca cctaaaaaga tgtgggcttg actattggga tatagctgat
    10921 atacaatatt acgatagctt tgaagaatta caagaaaagt atccaaatag taaatacttt
    10981 ttttctacaa caaaggctaa acagtcacat tcagatgtga aatatgaaga aaattgtttt
    11041 atagtatttg ggaaagaaac aaagggatta ccagaacctt tgttgaaagc aaatatggat
    11101 acttgtatta gagtgcctat gttggatgtt gaaaaggcta ggtcattaaa tttatcaaat
    11161 tcagttgcta ttgtagtgta cgaggcttta agacaaatag gatacccaaa tatgaggtag
    11221 tttcaacaag cctaaatgag aaaggagcat acttaatgaa aaatctaaag ataatctgtg
    11281 acagtttatc ggacgtgcca aaagatttgt tagataaata tgatatagat atagtttcat
    11341 taacagttat actggaggaa agagaataca aggatagaat tgatatatct ggagatgaat
    11401 tttataaaag gcttagagaa gaaaatgtat atccaaaaac ttctcaagct acttatgccc
    11461 aatttaagga aatttttgag aaatatacaa atgaggatag agagatactt tatatagcag
    11521 ggtctgccac agctacaggg acgtatcaaa gtgctgtaat ggcaaagaat gatgtggatg
    11581 gtaaaatata tacatatgac tctaacttgc tttgtatggg aactggttta cttgtaataa
    11641 aagcaggaga acttgcaagt gaaggaaagt ctgttgatga aatattacct gttttggatg
    11701 aattaaaaga gaaggctttt gttatatttt cagtagatac attggaatat ttacaaaagg
    11761 gaggtagaat atcatctact aaggcagcaa taggaagtat attaaatata aaaccgattc
    11821 ttgaaataag agatggacta gtttcgcaaa tatctcaagt aagaggaaag aagaatgtaa
    11881 tttctaagat gatggaattg ataaaatcaa attgtgggga tgatttatca gaccaaattg
    11941 tgtatatagg atatagtgat gattttaagg aaaaagaaca attatctaag gttatgcaag
    12001 atgaattaag acctaaagaa attagatttt tccaaattgg tgtgtgtata ggtgcgcatg
    12061 ctggtcctgg tgtcactggt atcatatgct taaaaaataa ataatatatt tttggatgac
    12121 attatttatt tttttattaa attaggatag aatattaatt attgagatgt taaattggat
    12181 ttattttgga tgaaattaag tgttgatata ttagatttag tttgtaggga agtgtgtaaa
    12241 tagataagaa tctattgcac acttttttat agtaagattt attaaaaaaa tttcaaaatg
    12301 tacttgtaaa ttgtatataa aaaaatt
//
LOCUS       DACTGW010000107        12228 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.107, whole genome shotgun sequence.
ACCESSION   DACTGW010000107 DACTGW010000000
VERSION     DACTGW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.107"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            184..1095
                     /locus_tag="I9573_002245"
     CDS             184..1095
                     /locus_tag="I9573_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HAT4796861.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            1355..1789
                     /locus_tag="I9573_002246"
     CDS             1355..1789
                     /locus_tag="I9573_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796862.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            1812..3347
                     /locus_tag="I9573_002247"
     CDS             1812..3347
                     /locus_tag="I9573_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796863.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVIYK
                     IDCDVILVNLFELIINNCIFSTILGNEVINLEISKYEYEFLEKQFKNMYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKTEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            3431..4798
                     /locus_tag="I9573_002248"
     CDS             3431..4798
                     /locus_tag="I9573_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4796864.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKARDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMNTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            4833..5057
                     /locus_tag="I9573_002249"
     CDS             4833..5057
                     /locus_tag="I9573_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HAT4796865.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(5507..5905)
                     /locus_tag="I9573_002250"
     CDS             complement(5507..5905)
                     /locus_tag="I9573_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HAT4796866.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMVG"
     gene            complement(5908..6342)
                     /locus_tag="I9573_002251"
     CDS             complement(5908..6342)
                     /locus_tag="I9573_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAT4796867.1"
                     /translation="MKSNYDCFRIAMLLKELYSKTMYTVEENFKENGLTHQQIIVIKL
                     VAHNQELTISQLCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFE
                     FATNFKIKMQESFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            6668..7654
                     /locus_tag="I9573_002252"
     CDS             6668..7654
                     /locus_tag="I9573_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4796868.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLNEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            7638..8549
                     /locus_tag="I9573_002253"
     CDS             7638..8549
                     /locus_tag="I9573_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4796869.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            8662..10230
                     /locus_tag="I9573_002254"
     CDS             8662..10230
                     /locus_tag="I9573_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT4796870.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHVLEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            10305..11330
                     /locus_tag="I9573_002255"
     CDS             10305..11330
                     /locus_tag="I9573_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796871.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDEYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            11323..>12228
                     /locus_tag="I9573_002256"
     CDS             11323..>12228
                     /locus_tag="I9573_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796872.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKS"
BASE COUNT         4846 a         1285 c         2181 g         3916 t
ORIGIN      
        1 ttatgaatta gtcatcaata aacaagaaat ttagctgtag aaatccatta taaaggctat
       61 tataaatttt aatatgctaa aaatataaaa agaatagttg acagtataga aaatatggaa
      121 taaactaaaa gtgtaatagt aatacgaaat tatatattac attgaaaggt gtgaatgatt
      181 attatgaata aaaaaataga attaataggg aattttgcaa gtgattggat taggtatagt
      241 gattatgaat ataaaattac tgaagaagat gaattatata tagttccaac agaagatgct
      301 gtgtttacca tgtataaccc atttgatgta gcagatgata taatagtaga tataattcgc
      361 ataggacaag aggcattaca agatatatca aaagagacaa aagaaatgaa actaaagaaa
      421 gaagtacttg aatttgtaaa aaagtatgga ttattgggtt taatatattc aagtgtatat
      481 aatagagata tagttggaga agaaagagta ctgatgattg aaaagaatta tattacaaaa
      541 gagaatatat tgagtacaga tgaatatgta aacatgttta taccatttgc aagtgatgaa
      601 gatgtgtatt ttaaaaaata tagaaggggt atagatgtat caaagagaga agaatctcca
      661 aagttttttg gaaagagacc attgatttta gatttagtat tttcaaagtt ctacactgaa
      721 caactagatt ggattgtaag ttttgctaaa gatatgtgta agcattttaa tcaaatttgt
      781 gtatataaaa atacatctaa tctattaact gaaagtgtta ctattatgtc tggagggttt
      841 aatcctcaaa agataggatt ttctataagc caattagata aaccaattat tgcatgggag
      901 tttgattctt tgaaaacagc tattgagact atctatgcct ttgcaattac aagtgaacct
      961 tcttctttaa ataaatgcaa gtactgtggt aaaatttata ttcctacaaa ttctaaatct
     1021 caatactgtt caccttcttg tagaaattgc tcaaatgtaa agaaaagtag aagtagagcc
     1081 aaaaaagata aataaaatta ataaatatat cattgtttag attttagtaa ataactactg
     1141 aattaaaagt tgttatagaa aattatatat ctaaatgatg gtattttttt gttgagaaaa
     1201 ttgaaaagtt taattgagat agtaattttg agttgacaaa aaccactttt aacactacta
     1261 ttataagtgg ttggtaaaat aatatacact aatttaaata tagcataaaa aacaaaaaaa
     1321 gtcaatgaaa aaaataaaaa aggagaattt aaaaatgata tcacctatta ttttgagttc
     1381 tataaatcag aatttaaagg aaatagaaag aaatgaactg cttgaaacta atatagaatc
     1441 tggagattat ggtctagctc tttctgaatc tgatgtaaaa gatataataa attcgagaga
     1501 taatacttta aaggggtatg gaagaataga attagatata aaagttacaa aacagttaat
     1561 agaaaatata tatacatcac aatatacaaa tgtggacaat tatttagagg ctattaatga
     1621 tatgcaagaa attttttatt atttaaaaaa tgaaactgat gacaagatat gtgatgatga
     1681 gattatagaa atactgggtg aattttatga aaaattttca ggcaatatgg acaatgtaag
     1741 aggagaagca gacgaatttg caaagaaatt taaatttgga gaagtttaat atatgatagg
     1801 agagattttg tatgttaaat aatttaagta atattaatat aaacgaaaat aatttaataa
     1861 agaaccaata ttccatatct ctacttaagg aatgtctaaa ttgtaaagtg atagatgaaa
     1921 gagaagtata taatatacaa caagaaatat ctctaatact tatggatttg attaaaaaat
     1981 atacaaatgg tcaaagcaca tcagtaaaaa cagaagttgc cgaaaaactg ttgatttcaa
     2041 tatggtatgc tattgatgca tatataaata agcttggaga tatagaaaaa agagttgtgg
     2101 agttgactgc taagaatgtt aaaaaagtgt accttggagg cattgaaata ttagaggaag
     2161 agttagttag gctgaaagat ttttataaac taatgatgag tagaaaattg gatactaatt
     2221 caatagcata taatgatacc ttgatggagt tatcaagttt ctttaaaagt tatattgtaa
     2281 aatttgaagc tcaggacgtt ccagtaagta tagattatcc attggcacta gatgatatgg
     2341 atgttaaggg tatttattat gtgaagaatt atattgagag tatagatata gaaaatagat
     2401 tttgtaacct atttaagaaa aaagatagag aaaaattatt ttatgactat ggggtgattt
     2461 ataaaataga ttgtgatgtt atacttgtaa atttatttga acttattata aataattgta
     2521 ttttttctac tattttagga aatgaagtga ttaatttaga aatttcaaaa tatgagtatg
     2581 aatttttaga aaaacaattt aaaaatatgt acaataatat tactgaagat aaagttatag
     2641 gctctgaaga aagtaatata attgaattga ttgaagtaga tgatagtttt aacactgaag
     2701 tagatagtaa tttggattat aatgattttg aagaaattaa cagagaaatt agaataaata
     2761 aaaaaataac tttactactt atggaagcaa ttgaagtatt aataaaaaga ttaagcatag
     2821 aagatgaaga tgtaataaga tatattaaaa aatatgaaaa gatatttatt gaatctgtgc
     2881 taagctcaat aaaaagtaac acttttaaaa gtatggtaac tgtgagtaaa aataaaaatg
     2941 aacacttaga agactatatt gttgatgatg aaagtaaatt ggatgatgaa tcgtttagag
     3001 agatattcaa tgagatatta gatagtacat ctataattga aaaaataaga ataataaagg
     3061 aaaatatcaa tgctaaaaaa gactttatcg atattcttga atctgaatgt ttatttggag
     3121 aagagtattt aatgttgttt gcatcattaa gtgaattaga attggcaata ttgggtagtg
     3181 tagtatttta tgaagatatt agaatgagag aaattaatat attagaattt atgttaaatg
     3241 agaaaactga aactacttta tggaagacag agtacattaa atttatgaag aggcaaagtg
     3301 aagataagat atactccata gaagagcata tgaataaaat taattaacaa aaaattgtga
     3361 ataaatagaa tgttagtata taatataatc tgtaaagcat atcaaaaata catatatttg
     3421 gaggaaaatt atgaaagagt atatcataaa taatgttgaa gaaataagag aagaattaat
     3481 tgatttatct aagaaaatat gggaaaatcc agaattagcc tttgaagaaa agtatgcaag
     3541 tagcattcaa aaagaatacc taaagtcaaa aggatttaaa attgaagaag tagaaaattt
     3601 acctactgga tttatagcaa gttttggaga gggtaaacct gtgattggca tacttggaga
     3661 atatgatgct ttaccagagc tatcacaatg tgtgagtgca gaaagaaagc cattggtaga
     3721 aggaaaagct ggacatggat gtgggcataa cctgttgggt gtagcaggtg taggagcagt
     3781 tgtttctata aaaaaattaa tagaagaaaa taaatttaat ggaacaataa aatattttgg
     3841 ttgtcctgct gaagaagaag gtggaggaaa gacagttatg tgtataaatg gctgttttga
     3901 tgatgtagat tgtgcattta catggcatcc ttttgatata aatgcacctt ggagaggtgg
     3961 aagtctggca aacttatctg taaaatttaa atttaaaggg ataacagccc atgcagctca
     4021 agctcctcat aatggtagaa gcgcacttga tgctgttgaa attatgaatg ttggagctaa
     4081 ttatttgaga gaacatgtta ttgactctat aagaatgcac tatgttataa ctaatggtgg
     4141 tggaagacct aatgtagttc ctggatttgc tgaaagttgg tactttatta gaggtaaaaa
     4201 agcaagggat gcagaacatg ttcttgatag attaataaaa gtagctcaag gagcagcaat
     4261 gatgacagaa actgaaatgg agtataaggt tacagatgga atatatgact atataccaaa
     4321 ccaatgtttg actgatttag tttatgacaa tatggttttt gttggttgcc ctaaaaatac
     4381 tcctgaagaa gaagaatttg caaaaaaatt gtgtgatact cttactagag aagaaagatt
     4441 aggtgtagca acatcattac aaaatgataa atctattgta gaaagttata tacacaagga
     4501 aatagttgac ggtgacaaag ataaaggact agctggttct actgatgttg gagatgtaag
     4561 ttatgtaata ccagtggctc aatttgcaat ggcagcttgg cctgttggaa ttgcaagtca
     4621 tacatggcag tcatgttcat cagcaggttc aaatatagga ttttctgcta tgataaattc
     4681 agctaaagta ctggcttgta gtgcttatga tgtatttatg aatactaaaa ttattgatga
     4741 agcaaaaatt gaatttgata aatcattaga tggacaaaaa tttaaaccat tggtataatt
     4801 ttggtaataa aatatttctg gggggaatag ctatgagaga aaaagttgaa aaagtgcttg
     4861 aggaaaagat aaaaccagta ttacaaagag atggtggaga tgtagagctt atagatgtaa
     4921 atgaaaatgg tgtggtttta gttagactac aaggggcatg tagtgggtgt ccaggagcga
     4981 ctatgacaat caaagctatt attgaaaatg ttttggtgag tgaagttcca ggagtaactc
     5041 aagtattagg tgtttaatag ttagcgttta ttaaatgtat gttatacata agtatttaat
     5101 aaagaagtaa aaaaaatagt gaagcaatat atatgaaaaa gtatgataaa gataataaaa
     5161 cattatagaa ataatgaaat atttcatatg tatatgaaga aataagaaaa aatagtgaag
     5221 caatatatat atgaaaaagt atgataaaga taataaaaca tttatagaaa taatggaata
     5281 tttcatatat acatatgaaa aaatagtaaa acaatatata tatgaaaaag tgtgagaaaa
     5341 taataaaata ctatagaaac aattaaatat tctatatagg tattaagaaa catgaaagaa
     5401 ataatggagt attatagata aatatgtaaa agtgtgaaag aaggatgtag ataaatttgt
     5461 cttatacaaa tttacctaca gtatttctta cacacttttt attttactat ccaaccatac
     5521 caataaacat acctataaag tatggaacta accatatagt ccatacaact atactaaact
     5581 tatgaaagaa ttttttcttt tcctcatcat ctttgtatag gacaaaagta gcccaacttg
     5641 catgaaaaag cataagcaca attgcaagaa gacctgtaat actatgaagg ttttgtgata
     5701 aagcagattt tacttcaaaa gtatggctgt tggctatgtt actcattgta aatgttccta
     5761 aagtatcaaa tataaaccca agccaaaata taattacatg tttctttttt aaaatttttg
     5821 cctttctttc tccaaataca cctatagtgt agaaaattaa agctgatgtt ataaatacta
     5881 ttgcaagtat taatttataa ttcataatta tctctccttt acttttgcca atatatttct
     5941 aagatttttt actaagtcac tcaattcgtt ttcatcacaa tttttaaaaa tatcatcaaa
     6001 actttcttgc attttaattt taaaatttgt ggcaaattca aatcctgttg ttgtaaactt
     6061 aacgtatgta tttcttttat cattagattt cttgaatttt tctatatatc caatctgttc
     6121 taatctgctg attatgcctg aaaccgtacc tttagctaaa gacatttcat cacatagttg
     6181 tgatattgta agttcttgat tatgagctac cagttttata actataatct gttggtgtgt
     6241 taatccattt tctttaaaat tttcttctac tgtgtacata gttttagaat aaagttcttt
     6301 taagagcatg gctattctaa aacagtcata attacttttc aataaaatca cctcttaaat
     6361 ggtttgcata caaactaata taatatactt ttcttatata gtcaacctaa aattttataa
     6421 ttttatagaa aataatgaag aatagaacat aaaaatttta ttttaggtat aaataaaata
     6481 atttgatgaa attttaacaa tttttaaaaa gtttgtttac acagttaata aatgatgcta
     6541 aaattaactc atagataaat atagaaaatt taattttgtt taatgataat ggaaccacga
     6601 aggttttaac gttactttac gtttgctttt atgtggtttt tttattgcaa caaagggggg
     6661 ttggggtttg ttaaaggata tactgaaaaa gttgttgcag tttattttag taatgttttt
     6721 attatctttt attgtttttt acatggcaag acttgctcca ggagacccat taatctctta
     6781 ctatggggat ggagtagaaa gaatgagtac acaagaaaaa gaaaatgcta tgaaaaaact
     6841 tggtttgaat gaaccaatat acagtcaata tataaaatgg atttcaaatg catcaaaagg
     6901 ggaatttgga atttcattta aatataaaca aaatgttact tctgttataa atgatgtcta
     6961 tataaacact ataatattag gtggttcagg gtatatactt acctttgtgc tagccttatt
     7021 gttaggaata ttttgtacat tacatgaaga taagctcatt gatagaatca tatgcaaatt
     7081 aggaacaata accaactgta ttccatcatt ttgggttgcc ttagtactca tacttatatt
     7141 tagtataaac ttaagtatac ttccaagtag tggtgcttat tcaatgggag aagagtccag
     7201 tatattaagt aggatatcac atctaatctt acctcttaca gtacttatat taagccatct
     7261 atggtattac acatatatga taagaaataa attactagag gaaataagag aagattatgt
     7321 gcttttatgt aaagcaaaag gtttaaataa tagaactatt gtttttaagc attgcctgag
     7381 aaatataatg ccatcatata ttagtataat ggccatttcg atacctcata tactaggagg
     7441 aacttatgta gttgaaaaag ttttttctta tcctgggtta ggaacacttt gttttgaaag
     7501 tgcaaagtat catgattata atatgttatt agttttatgc ttaataacag gggctttagt
     7561 agtatttggg aatatgttag ctcaaattat aaacaataaa atagacccta gaatgaaata
     7621 tgatagaggt gatactaatg aagccacaat atagtgattt tgaaattgta ggagaaaatt
     7681 atattttagt agcagaagat gaacatatag aaacaaagaa aaccttatct aaaaaaatta
     7741 aagaattacc atatatatca attattattt tatcaatcat agtcattgga agtgtattct
     7801 catcttttat aatgacacat gaaccaacat acatggattt agcaagttct aatttagctc
     7861 caaataaaac ttttttattt ggaacagact caatgggtag agatatatat tctatgatat
     7921 ggtatggagg aaaaatatct ttatttatag gactattttc cactgtaata tcaactgtta
     7981 taggaattgt ttatggtagt ataagtggtt cagcatctga actcgtagat gatgcaatga
     8041 tgaggtttac agaaatcata cttagtatac cttcaatatt aattataata tttgtacaag
     8101 ctatacttgg aaattcaaat ccagtatcta tgtccatagt aatagggata acaagttgga
     8161 tgaatatatc taagatagtg agaacagaag ttagacaaat aagaaatagc gaatacatac
     8221 tagcagctaa gagcatggga ggaggattct tctatgtatt aaagcaacat ttacttccaa
     8281 attttgtggc atcaataatg ttcatggttg taacaaacat aggcgcagct attggtactg
     8341 aatcgacact tagcttttta ggaattggtt taccaataga aattgtttct tggggaagta
     8401 tgttatcttt gtcagaggaa gcattacttt caaatagatg gtggattata ctgattccgg
     8461 gtatattttt ggttaccaca ttggtgtgta taacaaatat aggtaattat ataagaaaga
     8521 ataataataa aaaatctagt aatttataaa tcagcatttg agtactaaat aagcaaatac
     8581 tgaggtattt gaaatatcta tttaaataaa tggggtcaaa gctttgaaaa atattttata
     8641 aaaagcaaaa gggggagcat catgaaatta aagaagttaa aagttttaag tttagtaatg
     8701 atacttagtc taatggcagg ttgttctagt ggaggagaca aagataagaa ggcagatacg
     8761 cctaaagatg gaaaggtact tgtttatgga agtaatgact atacaagtat aaatcctgca
     8821 ctatatgaac atggagaaat aaattcatta atatttaatg gattaacagc tcatgatgaa
     8881 aataataagg tggttccttg tctagcaaag gactggaaat ttgatgaggc aacaaataca
     8941 tatactttta atttaagaga tgatgttaag tggcatgatg gagagaaatt tacggcaaat
     9001 gatgttaagt ttacaataga aactataatg aatccagata atgcttctga gatagcatca
     9061 aattatgaag atataacaaa aattgatgtg gttaatgata atactataaa aattacttta
     9121 aaagctccaa atacagcaat gcttgattat ttaacagtag gagtattacc aaaacatgtt
     9181 ttggaaggta aggatatagc tactgatgaa tttaatcaaa aaccaatagg tactggacca
     9241 tttaaacttg aaaaatggga caaaggtcaa agtataacac ttgttaagaa tagtgattat
     9301 tttgtaaaag aaccaggatt agataaagta gtctttaaga ttgttccaga tgataaggct
     9361 aaggcaatgc aattaaaatc aggagaatta gacttagctc aaataactcc taaagatatg
     9421 tctaactttg aaaaagacga aaagaatttt aaagtaaata taatgaaaac agccgactat
     9481 agaggtatat tatacaattt taattctaaa ttctttaaag ataaaaaagc taaaggatta
     9541 ccaaatgctt taagctatgc aatagataga aaagcaatag ttgacagtgt attattagga
     9601 catggtgtac cagcatattc acctttacaa atgggaccat ataataatcc tgatatagaa
     9661 aagtttgaat ataatccaga aaaagctaaa caagaaattg aaaagctagg ttggaaatta
     9721 ggttctgatg gaatctatga aaaagaaggt actaagttag ctttcgagat aacagcaggt
     9781 gagagtgacc aagttagagt tgatatggct aaaatatgtg cacaacagtt aaaagaaatt
     9841 ggtgttgatg ctaaagctgt tgttgtaact gaaacagatt gggctaatca agatgctcat
     9901 ttaataggtt ggggaagtcc atttgaccca gatgaccata catataaagt atttggaaca
     9961 gataaaggtg caaattacag tgcttactct aatcctacta tagataaaat acttcaaaaa
    10021 gcaagagaaa cagaagataa agatgaaaaa ttaaagttat ataaacaatt ccaagtagaa
    10081 atgactaagg atatgcctta tacatttatt gcttatatag atgcaatata tgttggaaaa
    10141 ccaaacataa aaggattaac acctgataca gttttaggtc atcatggtgt aggtatattc
    10201 tggaacatag ctgactggac aattgaataa tactgaaatt aattagaaac cttagggata
    10261 aaaatatatc cctaaggttt gaatagcata ggggttgatg gtttttggaa catttattgg
    10321 aggttaataa tctatctgta agttttaaag tagaagaagg agaagttcag gctgtaagaa
    10381 atgtatcttt taatctaaaa aaaggtgaga cattggctat agttggagaa tctggttgtg
    10441 ggaaatctgt actgtgtaag agtttgatga ggatactccc atacaatgga tatattaaaa
    10501 atggagaggt tttacttaaa tcaagtgatt tggttaagaa gtctgaaaaa gagatggaag
    10561 atataagagg aaagaatata tcaatgatat ttcaagaccc tatgacatct ctcaatccaa
    10621 ctatatcaat aggaaagcaa attgcagaag ctgtaatcat tcatcaaggt ataagtaaaa
    10681 gtgaagcaaa aaagagagcc atagagctta ttgaattggt tggaattgat aatcctgaga
    10741 aaagatttaa acaatttcca catcattttt caggtggaat gagacaacgt atagtaatag
    10801 ctatagcact tgcctgtaat ccagatgtat tgattgctga tgaacctaca actgctttag
    10861 atgtaactat acaagctcaa ataattgatt taataaagga tttacaacat aaaataggtt
    10921 tgtcaataat atttataact catgatttag gagtagttgc aactatagct gatagaatag
    10981 cagttatgta tgcaggaaag attgtagaaa taggaactgt agaggatata ttttatgacc
    11041 ctagacatcc atatacttgg gggttgttgg gttcattacc tactttagat tcacaggatg
    11101 aatatttata taatatacct ggaatgcctc caaatttatt gaacccacca aaaggagatg
    11161 cttttgcaat aagaaataaa aatgcactta agatagatta tgaaaaagag ccaccaatgt
    11221 ttaaaattaa tgatacacat agtgcagcaa cttggttatt gcacccagat gcacctgaag
    11281 tagatgtacc agttagagta aattgtggaa gggtgatttc taatgaatag agaaaaaata
    11341 ctggaaataa aaaacttaaa gcaatatttt catttggaca aaagtactac tgtaaaagca
    11401 gttgatgata taagttttga tatatataag ggtgagatat ttggattggt aggagagtcg
    11461 ggttcaggaa aatctacaac tggtaaaact ataataagac tacatgaatc tactggtgga
    11521 gaagtaattt acaaaggaaa ttgcatatca gataaaaaaa cgtacaaatt tataaaaaaa
    11581 gatgtaaaca aaagtatgca gattattttt caagattcta cttcatcatt aaatccacgt
    11641 atgacaattg cagatataat ttcagagcca ctaaaaattc aaggtatctg taaaaataaa
    11701 acagatagaa tgaataaagt atatgagatg ctaaagctag taggacttga tagaagttat
    11761 gcaaataagt atccatctga tttttcaggt ggacaaagac agcgtattgg catagcacga
    11821 gctctttcag tagacccaga atttataata gctgatgagc ctatagcttc tttggatgtg
    11881 tctatacaag cacaaatagt aaatttattt aaaaaattgc aacaagaaaa gaatttaact
    11941 tgtttattta tagctcatga tttatctatg gtgagacata ttagtgaccg tataggagtt
    12001 atgtataatg gaaaattagt agaattggca gattcaaatg aattgtataa taatcctatt
    12061 catccatata caaaatcact actttctgca attcctgttc ctgaccctag atatgctaag
    12121 tcaagaaata gaatagagta taattctaat ggatatgatt gttcaaatga aaagtcatta
    12181 agttggattg aggtttcaga tggacatttt gtatattctt ctaaatca
//
LOCUS       DACTGW010000108        12182 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.108, whole genome shotgun sequence.
ACCESSION   DACTGW010000108 DACTGW010000000
VERSION     DACTGW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12182
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.108"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            147..509
                     /locus_tag="I9573_002257"
     CDS             147..509
                     /locus_tag="I9573_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAT4796873.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
     gene            575..1192
                     /locus_tag="I9573_002258"
     CDS             575..1192
                     /locus_tag="I9573_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HAT4796874.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            1189..1674
                     /locus_tag="I9573_002259"
     CDS             1189..1674
                     /locus_tag="I9573_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HAT4796875.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            1717..2235
                     /locus_tag="I9573_002260"
     CDS             1717..2235
                     /locus_tag="I9573_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HAT4796876.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            2371..3330
                     /locus_tag="I9573_002261"
     CDS             2371..3330
                     /locus_tag="I9573_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT4796877.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNTRTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            3356..5059
                     /locus_tag="I9573_002262"
     CDS             3356..5059
                     /locus_tag="I9573_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HAT4796878.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINRV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            5316..7418
                     /locus_tag="I9573_002263"
     CDS             5316..7418
                     /locus_tag="I9573_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HAT4796879.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEAIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKNVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQINEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            7431..7871
                     /locus_tag="I9573_002264"
     CDS             7431..7871
                     /locus_tag="I9573_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAT4796880.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            7907..8710
                     /locus_tag="I9573_002265"
     CDS             7907..8710
                     /locus_tag="I9573_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HAT4796881.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKSFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            8750..9346
                     /locus_tag="I9573_002266"
     CDS             8750..9346
                     /locus_tag="I9573_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HAT4796882.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGLFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            9452..10315
                     /locus_tag="I9573_002267"
     CDS             9452..10315
                     /locus_tag="I9573_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAT4796883.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            10382..11371
                     /locus_tag="I9573_002268"
     CDS             10382..11371
                     /locus_tag="I9573_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAT4796884.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NRESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            11463..12074
                     /locus_tag="I9573_002269"
     CDS             11463..12074
                     /locus_tag="I9573_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAT4796885.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         4928 a         1199 c         2302 g         3753 t
ORIGIN      
        1 aagagaatta agtataatat ggcataaata attactttta tttatcaatg aaatgtattt
       61 attgtgtagc gaaaaatagt cagatagtat agacaataac tgaagataaa agcgtttatt
      121 gttgtacata tggaggagtt agttttatgg aaaataaaat tttgatagta gatgatgcta
      181 tttttatgag aatgataatt aaagaagctc tgagtaaaag tggctataat aatttaatag
      241 aagcaaatga tggagaagaa gcgtgtaata tatttaaata tgaaaagcct gatttgatgt
      301 tgcttgatat tacaatgcca aaaaaagatg gtttagaggt gctaagagaa ataaaaaagt
      361 tggattcaag tgctaaggtt gtaatgtgct ctgcaatagg acaagagaat atgattgtag
      421 aagccataaa acttggagcg ttagatttta tagtgaagcc cttcaaaaca gaaacattag
      481 ttaaagttgt aaataatgtt ttaaaataaa agtgtaaata tacactgata caatgaaaag
      541 caaataaatg caataataaa agggaggggt ttatattata aattatttag aacttgatga
      601 tgttcatata gatgctctta gagaaattgg taatataggc tctgggaatg caattactgc
      661 acttgcatcc atgataaata gcaatgtaga agtattaata cctatggtca agatacttga
      721 atataatgaa gcgacaaatt tgcttggtgg tccagaaaac aaagtagtat gtattttact
      781 cgatatgaaa ggtgatatca atggaatgtt tatgtttttg ttggatgaat caataactca
      841 acttatgcta agttctcttt ttaataaaga agaagctttt ttagatgaga ttgaggcaat
      901 tgaaatttca gcaattaagg aaataggaaa tattatggca agttcatatg taaatgctat
      961 tgcatctatg ttaaacatga ctataagtgt ttcaattcca gatatatgta ttgatatggt
     1021 tggagctgtt ctaaatgtgc ctatgataag attttcagat gttggtgaca aagtcttatt
     1081 tatagaaaat aaatttaaaa tgagtgataa ttattttact agccacattt tgatgattcc
     1141 agaaatgagc tcacttagag aaatactcgt gaggttggga ttagaagtat gaatagagag
     1201 atagtagtga acattgcaga tatgaagata gcctatagac caaatgtttt agttacttat
     1261 gcacttggct cttgtgtagg agtatgtttg atagataaag tagctggaat tggtggaatg
     1321 ttacacgtta tgttgccata tagtaaagat gctattaaca ttgaaaataa gtgtaaattt
     1381 gctgacacag gaataaatga gctcattaaa tctatggaga atataggtgc aaatagaata
     1441 tatataaaag caaaaattgc tggaggtgct cagatgtttt taggaagcag caattcagta
     1501 attgcaagta taggacatag gaatgttgag gcagtaaaaa aaactctttt aggattaaat
     1561 attccaatat tagctgagga cacaggtatg aattatggta gaacaatacg tttttacact
     1621 gagacgggtg aattacttgt agattcaata aataaaggta agaagaaatt ataatatata
     1681 tttcatagaa agtgtatttt aggagggagg tattaaatga gatttccaga tgacccatat
     1741 ttagattatg gactaattga tgaaggaatt aagtatctaa agtttaaagt agatgagcaa
     1801 gattttgggg ttgaacttga aaaggtaata gaaattactg gaaatcaaga aattatattt
     1861 attccagaac tacctacata ttctaaaggc ataataaatc taagaggaaa aatcattcca
     1921 ataatagata tgcgtttgag acttaaaaaa gatgaattaa tgactacaaa ttgcatgtat
     1981 atagtagtaa ctgaaataaa ggacttggta gttggatttg tggttgatag agttgatgat
     2041 atagtaagct tagaaaaaag taaaatatca cctccaccaa gagttactat agaatattcg
     2101 gattattttg taagtggcgt tggagaatta gaaggtaaaa taattacttt actagattta
     2161 gagaaacttt tgacagataa agatgaagtt ttaatagatg aattaatcaa tgggtttgat
     2221 tgcactatca tgtaacatag ggatgtagtt ggagtgttat tataaaaaga ttgtgatgag
     2281 aacatttaag aatatagtgg tataattatt aagtataaaa attttaaatc attaaaattt
     2341 gaatatatta tgtttaagag aggtttgtat atgaaaaata agcaacaggg aacaatttta
     2401 gtcgttgatg atagtgttct gatgtgtgac ttaatagata aaacacttac aaaagataat
     2461 tttaaggtta aaaaagcatt taatgcaata gaggcaaaag aatttattga agaatttata
     2521 ccagacatta ttttgcttga tataatttta ccagataact ctggttttga gttttgtaag
     2581 gaagtaaaat ctaatactcg tacagcagat atacctataa tttttataac ttcaaaaaat
     2641 acagatacag acatagtaaa aggatttggt gtaggagcaa ttgactatat ggtgaaacct
     2701 tttagtatga cagaattaaa agctcgtgtt atagctcatc ttgaagtaaa gaaatctcaa
     2761 gataaattaa aaaagataaa taatgaactg gaatcttctc ttgaaaaatt gaatagactc
     2821 gttgtaagag attatcttac aggtctttac aataggcgac atataattaa tcagcttatg
     2881 gagcaaagaa gaattaataa atatagtgaa acatcaatat cccttatcct tggtgatatt
     2941 gatgacttta aggttatcaa tgatacttat gggcatgaag caggggattt cgtgttaaca
     3001 gtaatatcaa gtataataaa aaagaattgt agacaaattg atactgtatc aagatggggt
     3061 ggagaagagt ttttgataat gctcacaaat actccaatag aaggtgctaa gatactttca
     3121 gaaagaatcc gtaaagagat aaaggagttt gatttcaact ataaagaaca taaaataaag
     3181 tgcactatta cattaggagt agtagaagta aactcaaata tatcattaga agaaaacata
     3241 tccttagcag ataaagcaat gtatgagggt aaaaacttag gaaaagattg tagcgtagtg
     3301 agtacaatga aaggattaaa aaagatataa acttacttta tgaaggagta cagatatgaa
     3361 aagagaagta aagctttcta aattagcaat tgtattgctg attattatag tattggctgt
     3421 tctagcaggg attttttctt ctatttcatc ttataaagca tttgaaaaag tagaaaaaag
     3481 tattaatgat atgaataatt tgaggactca aagtcagtat ataaaagata caactagaga
     3541 gataagtgat gtattaagaa gatatgtatt tacaggagaa aaaaagaata aagatattta
     3601 tgaaaaagaa ttatacggaa aaacaagaga atcagcttta aacaaattag aaaaaattgg
     3661 tcttacaaag agcgagtcaa ataaaatctt agaagcaaaa gaaatatcag attctttagt
     3721 accaacagaa caaagtgcaa taaaggcagt cgaaagtaat gataagagta aagctcaaag
     3781 tataattttt ggtagtgact atataaactc ccaagagaaa atggattctt taataaatga
     3841 atttcaagag gatatagtta cccgttcaaa ttccaaaatt gtagaagtaa aaaaagaatt
     3901 acaaagtcta atattaagaa caatatttat actttccctt ttaataatat tgacaataat
     3961 agaatataca gtaataaaat taaagatagt gattcctatt aatagagtag aaaaacactt
     4021 ttcaagaatt gcatctgggg acttaagaac aagtatagac gttgaagaaa gtaaatctga
     4081 aataggacta ttagtgagtt ctgtcaaaaa gatgcaaagt atgcttttaa attacataga
     4141 ccttatagac agtacattaa ctagtattgc aaaaggagat ttaagagtag agatagacca
     4201 agaatttata ggtgatttta aatcaattaa aatatcttta gaaagtataa taaactcttt
     4261 taatggtgat ttttctgaaa taaatttatc agcagaacaa gttgcaagtg cttcagaaca
     4321 agttgcagca ggtgcacaag ctctttcaca gggtgctact gaacaagcta gttcagtaga
     4381 agaattagct tccacgatta atgagatatc agatggaatt aagaagtgtg cagatagtgc
     4441 tagtcttgca tgtgaggtat ctgaccaatc tgcatcacaa gttggttttg gaagtagatg
     4501 tatggatgat atgatgacat caatgggtga aataagtgaa aaatcgagtg aaatatcaaa
     4561 aataatcaaa acaatagatg agatagcatt ccaaactaat attctcgcat taaatgcagc
     4621 tgttgaagca gcaagagcag gtcaatatgg tagaggattt gcagttgtag cagatgaagt
     4681 taggtctttg gctggtaaaa gtgcaaaagc agctcaaaat acagctattc tcatagaaga
     4741 agctattaaa acagtagaaa atggtgtcag aattgctaaa gagactgcaa atgcattgga
     4801 cttggtagta aatggtgtag aaaaaactac taattatatt aatggtattt cttcggctgt
     4861 acatagtcaa gaagtagcaa ttacacagat tttatctgga atagagcaaa tttccatagt
     4921 agtccaaatg aactcagcaa cagcagagga aagtgcagca gcctcagaag agatgtctgc
     4981 tcaagctcaa attttaaaag aattagtaga aaaatttcat ctaaaggaaa gtgaagtaag
     5041 agaatcatca tgggtttaaa ttatgaggtg aatattgaag taagagaatt atcatgtatt
     5101 taaattatga ggtgaatatt gaagtaagag aattatcatg tatttaaatt atgagatgta
     5161 tatatattga aataagagaa tgattgtgta tttaaattat gaggtacata tagtgagata
     5221 agagaatcat caaggattta aattatgaga tgcatatata atagaaaatt atttgtaggt
     5281 cgtaggtgat aaatgtgaat tatattgaac cgagtatgag acaaatgtta gatatgttta
     5341 tatttgagac aagtttatta ctcgaacaat tagatgaaat tatgattcaa atagaaaaag
     5401 agaaagtatt tactcataac aacattaatg aaacttttag aataatgcat acgataaaag
     5461 gttcagcatc tatgatgggg ttagacaata tatctgcttt agcacatagt gtagaagact
     5521 tgttttatgc tatacgtgaa ggtaaaatag aagttttaga caatgaccta ttatttgatt
     5581 tagtctttca atcattagat tttataagaa gagatataga tgagctacaa tcaagaggtt
     5641 gtatatcttc tgattactct aattttattg aaggaattga agagtatgtg agtactttgg
     5701 aattatcaag ttctatgaaa tcaaaggata atgcaaattt agaaagtaca gacgaaaaag
     5761 aagcaatcga ttcaaatttt gaatacaaag aagactctga tgtagtaaaa ataatctttg
     5821 aacaaggttg catgatggaa aatgtgagag cgttcatgct tgtatttgaa cttagagatt
     5881 actgtgaatt tatagatttt tatccaaaag atgtagaaac aaatgcagag tctacagaat
     5941 atattaaaag ttatgggttt tatatacaat ttgtagctaa agaggataag gcaatagttt
     6001 ataaaactat agaagataat ctctatgtaa aagaatacat aaatattagt aaaaaagaaa
     6061 tagaaatgaa aaatacatca aataatgatg cagtagaaaa taaattaaaa gataataata
     6121 aagtaagtaa aagttatcaa aataactata cagaagatga gatacaagaa gatgttttaa
     6181 atgaaaaaac tgatatggaa aaagatagtc taagtgaaga aaatgaacat ttaccaataa
     6241 gtaagcagag tattatgagt gtaaatttag aaaaattgga tttacttcaa aatatagttg
     6301 gagaaattgt aattatggaa tcaatggttg taaatagcgt aaattctaca ggtgttgata
     6361 tagataattt caataaggca acaagacaat tacacaagct tacagatgaa ttgcaagata
     6421 tagtaatgtc tatgcgtatg attcaaattg ctggtgtatt tcaaaaaatg catagaattg
     6481 tacgagatat gaataaaaaa ttagataaaa atgtagagtt aataactata ggagcagaga
     6541 cagaggtaga taagaatgta attgataatt tagcagaacc ttttatgcac cttataagaa
     6601 atgctatgga ccatgcaata gagcctattg aggagcgtat agcgaaaggt aaagaagaag
     6661 tgggaaagat agttttatct gctgagaatc taggcggaga agctgtgata agtatttcag
     6721 atgatggtag aggcattatg agagataaga ttttggcaaa agctaaggaa caaggtcttc
     6781 ttaaacatca aattaatgag tatactgatg acgaggtcaa ttctttgatt atgactccag
     6841 gtttttctac aaaagaatct ataactgaat tttctggaag aggagtaggc atggacattg
     6901 ttcgtaaaag tattgaaaaa gttggaggtt caattgaagt aaaaagtaaa caggatgaag
     6961 ggacaacctt tactataaaa ataccattaa ctctttctat agtagagggt atgaagttta
     7021 aagttggaga ttcactattt actttaccaa tatcatccat aagaagaaca tttaaactta
     7081 cagacttaaa tcaaataatt acagaaggaa ataagacaga gttaattaca atatatggag
     7141 catgtattcc cattataagg ttacataaaa tatataatat tcaaacaaaa gtaaccaatc
     7201 tgatgcatgg taatatgata attattgaaa atgagcataa agcagtatgc atttttgttg
     7261 atgaaataat aggtaatcag caagttgttg tgaaaccatt tccagtttat ttcaaccgat
     7321 ttaatataaa aaataagggt ttatcgggat gtacaatact tggggatgga agtataagtt
     7381 taattataga tgcagatagc attatagaaa tatattaggg gatttaaagt atggctaaag
     7441 atgaaattat taaacaagta ttaacatttt atgtaaatga tgttatttat ggaatagaat
     7501 tagaaaatgt gattgaaacc ataagatttc aatcaataac gtatgtacca tgtcttccaa
     7561 catatataag tggagttatt aatttgagag gaagaataat accagttatt aatatgcata
     7621 taaagtacaa tcttcctaaa gctgattata atgagagaac ttgtataata attacaaagg
     7681 ttgatgagta tcaagttgga attattgtag ataaagttgt tgatgttatt catatagaaa
     7741 atctaaattt actagagacg actaattcta acaacactaa tattaataaa gatataaaag
     7801 agattgcgaa agttgaagat aattctatat taattttaga tattcgaaag tttttgatta
     7861 atagcatata gttagttctg ataaattagc aggaggaagt ttttgcatga taaaactcac
     7921 agatgaagaa tttatagttc ttgtaaacca tgtaaaaaaa gaatatggta ttgatttaag
     7981 taaaaaaaga gcgttaattg aaggtcgctt atacaataca atgatagaga aaaaacttag
     8041 ttcttttagt caatatatga atctattatt taaagataag acaggaaatg aagctataaa
     8101 cctaataaat agattgagta ctaaccacac attttttatg agagaacctc aacattttga
     8161 gtttatacag aatagtatac ttcctttttg ggaagaaaat aataaaataa gaaacataaa
     8221 tatatggagt gcaggatgtt ctagtggaga agaagcgtat tcaatagcta tgactttgga
     8281 tgattatttt ggatataata aagagttgtg gaacattaaa atcatagcca cagatatttc
     8341 aactaactca cttgaaaaag ctaaaagagg tatatacata gaatcaaatg ttaacaatgt
     8401 tcctgagtta tggaagaaga agtattttat agacaacaag gatggtacat ttaaaatttg
     8461 tgataagatt agaaaaagtg taatttttaa gtcatttaat ttaatgaatg atttctcata
     8521 tcaacatttt gatttgattt tttgtagaaa tgttatgatt tattttgatt taaaaacaag
     8581 agaagaactt ataaataaat tttataatgc taccaaaaaa gatagttttt tgtttgtagg
     8641 acatgcagaa gtaatcaaca gaagtagtac taaatacaaa tatgtaaaac ctgctatata
     8701 taaacgataa aaaggtattt ttgatatatg attttttgga ggcggtaatg tggaacgttt
     8761 taataatatg gttattgcta taggcgcatc tgtaggtggt acagaagcaa tacttgaaat
     8821 tataaaagac ctaccaaaat caacaccagg gattgttgta gttcaacata tgccagctat
     8881 atttacttat atgtatgcac aacggcttga caagcagtgt attatgaatg taagagaagc
     8941 taagaataat gatagagtcg aacaaggaaa tgtgttaatt gcacctgggg gttaccagat
     9001 gaaattgtgt acggataaac aaggatatta tgtaacttgt gaaaaaggag aaagaataag
     9061 tggtcactgt ccatcagtag acgttttgtt tgattcagta gcagaagtag caggtaaaaa
     9121 ttcaatagga ataatactta caggtatggg ttatgatgga gcaaatggac ttttaaaaat
     9181 gaaagaaaat ggtttattta caattggaca ggatgaaaat tcttgtatag tatatggaat
     9241 gccaatggta gcttttgata gaggttctgt tatgttacaa ttacctcttg gtgaaatttc
     9301 aaactgtttg ataagacata ttaatatttt aaaacaaaaa gattaatttt ttataaataa
     9361 aaattaatct tttattaaat tatatggata aaaaacccta gaaactccaa atataataag
     9421 agagatttat atttaggagg aaaatgataa tatgaaattt tttaaagtcg atacaaaagc
     9481 taaagtttta agatatgtat ttgttttaac attgactata atggctgttt tggcaattaa
     9541 acatgactat aaatataata atttaaggaa agatgcttca attaatttaa atagtaataa
     9601 ggaagcagtg actgttatga gtaaccagtc attaccaaag ggtaatacaa atagtaaaca
     9661 aactttatca gtaagtacaa taggaagtaa aacaatagtg aaaaatgatg agtatttaga
     9721 aagtactata aacatgcctg taataacaaa ttcaaataaa attgttgaaa ggtctacaaa
     9781 tgataagata aaaaatgaca tatttgaatt ttataataaa tcatataaag aagcaaagca
     9841 gtatcttaag gataatcctg acgagaaaaa taagtttgtt gcaaatgtag actttgaact
     9901 taaaaagaat actgattctg ctcttagtat aaaagtgaga tattatactt atagtggtgg
     9961 agcacatgga ttttatcaag atatagcata taatgtggat atgagaactg gaaaattttt
    10021 agaattaatg gacttattta aggataatac taaatataaa gaagttatag atgaagaaat
    10081 taaaagacaa atagctgaac ttgaaaaaaa ggatgaagaa aatatcggaa tatataattt
    10141 taaaggtata aaagagaatc aaaactttta cttgcaagat gagaatctag ttgtatattt
    10201 tgatttatat gatataactc catatgcagc gggaatacca gaattttcaa taaataaagc
    10261 gttgataagt tctatgctaa aaccagagta tgtagattta tttgatttga aatagaagat
    10321 taaattaaat gttatgctat aatatttagc atagaaactt attaaaattg ggggtataaa
    10381 aatgctactt ttaaaaaaag acgacataaa aaaagtattt acaatgagag atgcaattga
    10441 agctgataag gaagctttta gaatttattg tgaaggaaag agtgtaaatc ctttgagaac
    10501 caatatatct gttccaagtc aagatgctag tatgttattt atgccaggat acgtagaaga
    10561 attaggctgt gggggaataa aaattgtatc tgtatttcct aaaaatgctc aaaaaggcaa
    10621 accagtaatt ccagcaacag tactattatt agatggagaa actggagaag ttagtgcagt
    10681 attggatggt acttatgtaa ctcagattcg tactggagca gcctctggag cagctataga
    10741 tgtattggct aaaaaagatt ctaagattgg agctttgata ggtctaggag gacaaggtga
    10801 gccacaactt gaagccatga tagaagctag aaatttggat gaggtaagag tttttagtag
    10861 aaacagggaa agtagagaaa aatttgcaga agaaatgaac ttaaaattaa gtaaatataa
    10921 tactaaaata gttgcagttg aatcatctga tgaagccata gataacgcag atgtgatagt
    10981 tttagctact ccatcaaaac aaccagtact taatggaaac ttagtgaaaa aaggagcttt
    11041 aataagtgca gtaggttcat atatgccaga tatgcaagag ttgtgcccaa actgtttaac
    11101 tagagcgtct aagatatttt ttgaatcaac agatgcagtt ttatctgagt caggagatat
    11161 attaattcca ttaaaagaag gtaagataag taaagatgat tttagtggtg atttaggaaa
    11221 cgttataaat ggaactattc ctggaagaga agatgatgat gagataattg tatttaaaac
    11281 tgttgggata ggtgtacaag atgtcgtaac agcaaaaaga atatacgata aagcaaaaga
    11341 aaacggtatt ggaatagact ggaagtggta aaatattcaa aataattcta tagttagaca
    11401 aatttatgtt ataatgttga taaataaatt ttttattatt ttaattgagg gaggatgttt
    11461 gtatgaaaat ggtaaaaaag ttaatattgt ctacgattat ggctctagtt ttagttctaa
    11521 ctatagggtg ttctaatgca gaagaaaaag taaagtcgaa ttttgaagct aatttagctg
    11581 cattagaaat gtataagtat gatgaaaaag gaatttctga tgcattaaca gaagctgaat
    11641 ataaacagat tcaaacttat aacagcaagg acaaggtttc aatgattgct caattgacac
    11701 ctctttatgg taaagaaaat gctgaaaaga tatacgaaac aattgttaaa ttaattcaag
    11761 gtgttgaaaa gacagtaaca gtggaatctt ctagtaagga taagataact ttgaaagttg
    11821 taagtaaagc tttagatact agtaaagtaa tgagtgaagc gaacacttta gtaaaatctt
    11881 atgtaactga tgaaaagaat tacgataaaa aagaaaaaga agttcaagat gatatgacaa
    11941 aaatgattat agacctttac aataagaaac caactaagga agttacaact atgattacat
    12001 atactaaaaa agatggtaaa tggcaaccac ctagtaatat acaaaagtta tgtgaaggaa
    12061 tatatacaat ttaatttatt ttaaagacac aaaactatct tttaagcata taataaaatg
    12121 tttggaagat agtttatttt attttcaaag tataaattat attttataaa tgatttattt
    12181 ta
//
LOCUS       DACTGW010000109        12137 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.109, whole genome shotgun sequence.
ACCESSION   DACTGW010000109 DACTGW010000000
VERSION     DACTGW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12137
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.109"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..336
                     /locus_tag="I9573_002270"
     CDS             <1..336
                     /locus_tag="I9573_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAT4796886.1"
                     /translation="KVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYNGLKE
                     AGIDSEITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYY
                     GILKSVYKK"
     gene            352..1935
                     /locus_tag="I9573_002271"
     CDS             352..1935
                     /locus_tag="I9573_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HAT4796887.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1963..3291
                     /gene="ssnA"
                     /locus_tag="I9573_002272"
     CDS             1963..3291
                     /gene="ssnA"
                     /locus_tag="I9573_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HAT4796888.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3490..4842
                     /locus_tag="I9573_002273"
     CDS             3490..4842
                     /locus_tag="I9573_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAT4796889.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            5034..6143
                     /locus_tag="I9573_002274"
     CDS             5034..6143
                     /locus_tag="I9573_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAT4796890.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6426..7817
                     /gene="hydA"
                     /locus_tag="I9573_002275"
     CDS             6426..7817
                     /gene="hydA"
                     /locus_tag="I9573_002275"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HAT4796891.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7811..10372
                     /gene="xdh"
                     /locus_tag="I9573_002276"
     CDS             7811..10372
                     /gene="xdh"
                     /locus_tag="I9573_002276"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HAT4796892.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10524..11870
                     /gene="rpoN"
                     /locus_tag="I9573_002277"
     CDS             10524..11870
                     /gene="rpoN"
                     /locus_tag="I9573_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAT4796893.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT         4814 a         1420 c         2477 g         3426 t
ORIGIN      
        1 aaagtttctt atgcagttga aaaagctgat tgctatacag gaaatactat agaaagtgaa
       61 agattcttcc caggttggtt atatgatgaa gaagatgaat ttgttcaagc agcgtataat
      121 ggattaaaag aagctggaat agattcagaa ataactcaat actcattctg tacgaatgga
      181 agtcactacg caggtgaggc tggaataaga actataggat ttggtccttc taaggaaaat
      241 cttgctcata caatagatga gtatatagaa ttagaccaat tattcaaagg tgcagaagga
      301 tattatggta tattaaaatc agtttataaa aaataattag gagatggttt tgtgagaact
      361 ttaataaaac atgggcttat agtagatgga aataaaacac cagcatatga aggagatatt
      421 ttaatagaaa acgagaaaat attaaaaata tcacaagatt taaacgaaga agctgacaaa
      481 gttatagatg caaaaggtag agttatatgt ccaggtttca tagatactca tagccattca
      541 gacctagtta ttttagttaa tccatataat gaggtaaaga ttagacaggg aattacaaca
      601 gaagtgttgg gccaagatgg tatatctatg gcccctctac ctcaagagca tatatcttct
      661 tggagaaaaa atttagcagg tctagatggt gaaagtgatg aaattgattg gaaatatgaa
      721 acaacagaga attatttaaa aatgatggat tacaatgggg taggtttaaa tgaaacttat
      781 ttagtacctc atggaaacgt tagaatggaa gctatgggac ttgaagatag acctgctact
      841 aaagaagaaa ttcaaaaaat gtgtgaaatc acagagagag aattaaaagc aggagctata
      901 ggattgtcta ctggacttat atatatacct tgtgcatact cattgactga agaaattata
      961 gagatgtgta aggtagtagc aaaatatgac ggagtatttg tagttcacca aagaagtgaa
     1021 gcagatacaa ttttaacttc aatggaagaa atcatagaaa ttggaaaaca atcaggagta
     1081 aaagttcatt tttcacattt taaggtttgt ggtaaagcaa actggaagta tataccacaa
     1141 gttatagagc ttttagaaaa agcagaaaag gaaggaataa gagtatcttt tgaccaatat
     1201 ccatatgcag caggaagtac aatgcttgga gtagttttac ctccttgggc acattctgga
     1261 ggaactgata agttaataga aagattaagc gatgaaaacg aaagagcaaa aatgaaaaaa
     1321 gatatagcaa atggaataga aggctgggat aattttatag aatttgctgg aatagaccaa
     1381 atatttgtaa caagcgtaaa aacagaaaag aataaagata caattggtaa gagcttatta
     1441 gaaattggaa aaatgagagg aaaagaccca ttagatgcaa cgtttgattt attaaaagaa
     1501 gaagaaaatg cagttgggat ggtagatttc tatggattag aagaacatat aataggattc
     1561 atgaaaagag acgagcaaaa tgtgtgtaca gatggtcttt tagcaggtaa accacatcca
     1621 agagcttatg gttctttccc taaaatacta ggaagatatg ttagagagtt gaatgtacta
     1681 acaattgaag aagctgttta taaaatgact aaaaaggcag ctacaagctt tagtataaaa
     1741 gatagaggcg aattaaaaga aggatatttt gcagatatag ttatatttga caaggataca
     1801 gttagtggtt gtgatgattt tatcaactct atgcagtacc ctacaggtat agattacgta
     1861 attataaatg gaaattgtgt tgttgaagaa ggaaaataca ataaaattaa agcaggaaaa
     1921 gtacttaaaa attaaatctt agctttaagg aggaataaat ttatgatttt agtggggaat
     1981 gggaggctaa taagccaaga ttctttaaat ccctatatgg aagatggatg tgtagtaata
     2041 aatgataatc taattgaaga tataggtact acagaagaaa tgaaagctaa atatccagta
     2101 catgaattta tagatgcagg caagaaaata atcatgccgg gtctaataaa ttcacatatg
     2161 catatataca gctcatttgc tagaggaatg gcagttccag gaaaaccttc tgagaacttt
     2221 ttagagatac taaataattt atggtggaga cttgacaaac agcttacact ggaagataca
     2281 aaatacagtg catatgcaac gtatatagaa tgcataaaaa atggtgtaac aacagtttat
     2341 gaccatcatg caagtccaaa tgcaattgaa ggcagtttgt ttacaatatc agatgttgca
     2401 aaggatttgg gaattagaac gtgtctttgt tatgaagtgt ctgatagaga tggtggaaat
     2461 acaatccaac aaggtatcaa tgaaaacgta aactttataa aatacgctca aaaagatgat
     2521 acagacatga taaaaggaat gtttgggctt catgctgcat ttacactatc tgatgataca
     2581 ttaaataaat gtgcttcaga gatggctggt cttgatacag gttaccatgt acatgttgct
     2641 gaaggactag cagacttaca atataatgta gataaatatg gaaaaagggt tttagaaaga
     2701 ttcaatgatt ttggagtact aaaagaaaaa acaatagcag ttcattgtat atatataagt
     2761 gcagaagaaa tcagtctttt aaataaaaca aatacatttg tagttcataa tccagaatct
     2821 aatatgggaa atgcagttgg atgttctcct gcaatagagt tattaaaaag gggagttaca
     2881 gtaggattag gaactgatgg atatactagc gatatgtttg aatcattaaa agttgccaat
     2941 ataattcaca aacatcactt aaaagaccca agagttggat ttgaagaatc acaaacaatg
     3001 ttatttgata acaatagaaa aatagctaaa gcttattata aaaatgacct aggagtactt
     3061 aaaaaaggtg cttatgcaga tgttatagta gttgattata taccacatac tccattgaca
     3121 gaaaatacaa tagggggaca cactattttt ggaatgagtg gaagaagcgt agatacaact
     3181 attataaatg gaaaagtact tatgaaagat agaaagttaa taaacataga tgaagaaagt
     3241 attctagcaa aaagtagaga attaagtcaa aatctatgga atagaattta aataattgat
     3301 tatgcaaaat tctatgaagc aggaaccctg tgactttagt catgggaggt tcagattaat
     3361 atacaagtaa gaaacaagaa tttagatgtt aaaaacagag cttaaacaaa aataggaatt
     3421 taaatgaagt atataaataa aaaatatacg gaatagaata ttaaattaaa tatagaaggg
     3481 agacccaaaa tgtctcaaga taaaagccct aaaatagctc cagtagatga aaggttacct
     3541 gtatccaaat cttgggtatt ttcactacag cacgttttag caatgtgtgc aggagcagta
     3601 gcagttccta tgatgatagg aagtgctgca cgactagatc atgctagtat tgttttttta
     3661 ataaatgctt gcttgtttat ggcaggtata ggaacactca tacaaagtgt tggtatagga
     3721 aatattgtag gtgctaaaat accagtaata gaaggaacta gttttgccgc tgtggcaggt
     3781 atgacagcaa tagcctcatc atatagtgat tcacaagttg caatacagac agtatttggc
     3841 tctgtaatag tagctggatt gttttgtata ttacttgctc cagtatttga aaaattaata
     3901 agatttttcc ctagtgtagt tactggaaca gttgttatgg taataggtat ttcactattt
     3961 ccagttggga taaaatggat aactgacaac aaattggaag cggttgaacc aaactcatta
     4021 gcattagctt tagcagtatt ggcaataact ttactttcat ttaaattctt aaaaggaatt
     4081 tggaatagca cagctatatt ggtaggtata gtacttggta cactactttc catggcagtt
     4141 ggaatggcag attttagtgc agtaaatgga gttggttggc ttaacttaaa tgtaccttta
     4201 aaatttggaa tgcctagatt tgaaccatct gcaatactat cattgtgttt gattatgttg
     4261 gtacttatga cggaatcagt tggtaatatg atagctattc atgaaatggt agacaaagaa
     4321 gtaacaggta aaaatattaa aagaggtcta tttggagatg gaatatctac agcattatct
     4381 ggaatattca attcatttcc aataactcca tttggtcaaa atacaggaat agtaggtctt
     4441 acaggtataa aatcaagatt tatagcagtt tacgcagggg ttatattgct agtacttgga
     4501 ttcttaccaa aatttgcagc aataatagga gcaataccta agcctgttct cggaggcgta
     4561 ggatttgcaa tgtttggaat ggtaatggtt ggaggagtaa aaactttaag caaagtgaaa
     4621 tttgatggaa ctaaaaatgg ggttatagta gcagttagtg ttggtttgtc tatgatacca
     4681 ctaacaaatc ccgatttcta tcataatttc ccaacttggg tacaaacaat attccatagt
     4741 ggaataacaa caggtagttt atcagcagtg cttttaaaca tattcttcaa tgaactaggt
     4801 tctaaaaaag aaaaaaataa taaagttgaa gaagcaaaat aaaaataaaa caagtaaaaa
     4861 tctatattga cttaaagtca ttaagcatta ataaaaataa ttcagagata ttaataattc
     4921 aacaaaacta tcttatattt taaataatat aaaaaagtaa ataaataacg aatacaaata
     4981 aaagaataca atctaaatat taaaaatatt taatttaagg gagagataat aatatggcta
     5041 atttaaaagt taatttatat ggaatggaat ttttaaaccc aattatgaca gcggcaggac
     5101 ctggagcaaa agatggtgct ctctgtcaag aggcagcaaa aggtggagca ggagctatat
     5161 gtacaaaaac tatatctgta ttaccagctg atgtaccaag accatgtatg gcaaaaacta
     5221 atagtggttt tttaaacaca gaattatggt cagagcttcc aaaagagcaa tggatagagg
     5281 aagaatacaa aaaggtaaaa gaaacagggc ttccagtaat agtaagtatg ggatattcag
     5341 cagagcaaat agctgaggtt gctcctttag taaagcctta tgcagatgca gttgaattat
     5401 ctactcatta tgtaggtaca gacgttgcac caatagtaaa tgctttaaaa gctgcaaaag
     5461 ctgttttaga tgtaccagta tttatgaaaa tgagtcctca tactgacatt caaactattg
     5521 caaaagctgt tgaagaagca ggtgcagatg gacttgtaat gataaactca tttggaccat
     5581 gtatggcaat agatgtaaat acaggatacc ctataatggg aagtaaagct ggatacggat
     5641 ggttatcagg tgcaccaata agacctctag caataagatg tatatatgaa gcttctcaag
     5701 ttgtaaatat accaataatc ggtgttggtg gaatcacttg tggaaaagac gtggctgaaa
     5761 tgatgatggc tggagcaagt gctgttcaag tatgtacaga agcaatcctt aaagggcctt
     5821 ctatctatgg aaaaattgct aaagaattaa atgaattttt agatgagaat ggatatgctg
     5881 atgttaatga aataaaagga ttaactcata agaaaatggc agctagaggg tttagaactc
     5941 attcagtagc tccagtagtg gatgataaca agtgcatcaa atgtaagcaa tgtgtaacaa
     6001 gctgtgtata cgaagcttta gaagttacag ataagttgaa catagatgca gacaagtgtt
     6061 ttggatgtgg attatgtgta actagatgtc caaaaggagc tttaagcatc tgctacaatc
     6121 aagtagaagc tatgaatgag taataattat atagaatttt ggtaattaac ttagagtaat
     6181 aggtattagg ataataaaat atatcagata aacaaacaca cattatgcat taataataca
     6241 gtctaaagtt aataatttta ttatattgat ttaactaaat attagtttag ctgaatatta
     6301 atttaattag gtattggttt aatcttaata tagatttact acgtataagt gaactaacta
     6361 gattacttta agttaatttt ttcatataaa ataaattgat acattacaag taaggtggag
     6421 tctatatggg aattgtttta aaaggtggga aagtagtaac aagtgaaaaa acttactgtg
     6481 cagatgtaag aatagaagat gaaaaaatag tcgatatagg tcttgatata tcaaaagaaa
     6541 atgatgaaat agttgatgtt agtgattctt atgttatacc aggaggtata gatacacaca
     6601 cacactttga cttagataca ggcgttacta agacagcaga taactttgag acaggaacaa
     6661 aagctgctat agtaggagga actactacta tattggattt tgctacacaa gctaaaggat
     6721 gttctttgaa agacggatta gaagaatggc atgaaaaagc taaggataaa tcctattcag
     6781 attatggctt tcatatggct ataacagatt ggaatgactc ggtttgtaat gaaatggagg
     6841 atatggtgaa agagggagta tcttcattta aactgtatat ggcgtataaa ggaagccttc
     6901 aagttgatga tggtgtaatc tttgaagcat tgagaaaagc agaagagata ggagggatta
     6961 ttggtttcca ttgtgaaaat ggggacataa tatgtgaact tgtggataaa gcaaagtcag
     7021 aaaataattt atctccaaaa tatcatcaat taactagacc agctataatg gaggcagaag
     7081 ctacatcaag acttatgaag atagctgaag ttgcaaactc aaaagtatat gtagttcact
     7141 taagctgtaa agaatcctta gaggaggttt taaaagctaa gcaaagaggt gtggatgtaa
     7201 tagttgaaac ttgtcctcaa taccttctat taaatgatga attatatgat ttagatggat
     7261 ttgaaagtgc taagtatgta atgtcaccac ctttaagaaa taaagaaaat aatgaaatat
     7321 tatggaatgc tattttagat aatcaaatag acactatagg aacagaccat tgttctttta
     7381 attataaggg acagaaggaa cttgggataa acgatttcag taagatacca aatggttctc
     7441 caggtgtaga acataggatg agtcttttat acacatatgg agtctgtaaa aataaaatat
     7501 ctatcaacaa gatggtagaa gtaacatcaa ctaatgcagc aaagttgttt ggaatgtatc
     7561 ctaataaagg aactatagaa ataggcagtg atgcagatat attagtctta aatccaaaca
     7621 aaaaatccaa aatagaagca aaaaaacaat tacaaaatgt agattataca ccttatgaag
     7681 ggtatgaagt ggattgtcaa atagaacatg tttatttaag aggaaaagaa gtagttagag
     7741 atggagaatt aatttgtaat actccaagtg ggagatatat aaataggcgt ctaacaaagg
     7801 gggctagaga atggtagaat ttatattaaa tggagagaaa gttgttgcaa aagaaggcaa
     7861 aaaactttta gacttcctta gagaagatat gaatataact tcagttaaaa atggctgttc
     7921 agaaggtgct tgtggaacat gtatggttat aatagatggt aaagctacta aagcatgtgt
     7981 atttaaaact gataaattag ctgggaaaaa cataacaact atagagggat tatcagagag
     8041 agaaagagat gtttttgcat attcttttgc tagtcaagga gctgtacaat gtggattttg
     8101 tattccaggt atggtaatta gttcaaaagc acttatagat gttaataata atcctactga
     8161 agatgatata aagaaagctt taaaaggtaa tatatgtaga tgtacagggt atgtaaaaat
     8221 aattaaagct gtcgatttag cagcaaagat ttttagagaa aatatagatg tgccaaaagt
     8281 aaactgtaag ggtctagtag gtgaaaatct tcctagagtt gatgcagttg gcaaagcact
     8341 tggatattct gaatatgtag atgatatgag aattgagggt atgatttatg gttcagcagt
     8401 tagagctaag tatcctagag ctttagttaa aaaaatagat atagaagaag caaaagctta
     8461 tccaggagtt gttgcagttc taacagctaa agatattcca ggaactataa aggtagggca
     8521 cttaaagaag gattgggata ctttaatacc agaaggtgaa ataactagat atttaggaga
     8581 tggaatagtt ttagtagcag cagaaactag agaagcgtta gaagaggcca aaaagctagt
     8641 taagatagat tatgaggaat taacaccatt aagtactaca gcagatgcac ttagagaaga
     8701 tgctccaaag atacatgaga cagggaatat acttgttaag gaacatttag taagaggaaa
     8761 cgctaaagaa aagatagaaa attcaaaata tgttgtaact aaaagatata ctacacctgc
     8821 tacagaacac gcatttttag agcctgaatg tgcagtagct ggtccttatg aagatgatgg
     8881 agtaataatc tattcaacag accaaggggt gtttgccact caacatgaat gtgcagatat
     8941 gttagggtta ccatatgaga aggtaagagt tataaacaaa atggtaggag gagggtttgg
     9001 tggaaaagaa gacatgagtg ttcaacacca tgcagcctta cttgcatacc atacaaagag
     9061 atttgtaaaa gtacagttaa caagaaaaga aagtattatt gttcatccaa aacgacatgc
     9121 cacagaaatg gaatttacga cagcatgtga tgaaaatgga tacttaacgg gtatgaaggc
     9181 aacgataata tctgacacag gtgcatatgc ctctcttgga ggaccagtgc tacaaagact
     9241 gtgtactcat gcagcaggac catacaatta tcaagatatt gatataacag gaacagcagt
     9301 atatactaat aaccctcctg gaggagcatt tagaggattt ggagttacac agagttgttt
     9361 tgcaactgaa tgtaatctaa atttacttgc tgaaatggtt ggaatatctc cttgggagat
     9421 aagatataaa aatgcaataa gacctgggca agttttacca aatggtcaat ttgcagatga
     9481 aggaacagca atggcagaga cactagaagc tgtaaaagaa gagtatgaaa agcatccacg
     9541 tgcaggtata gcttgtgcta tgaaaaatag tggggttgga gttggtatac ctgatgttgg
     9601 aagatgtaga ttggtgataa aagatggcaa agttcacata agaacaagtg cttcttgtat
     9661 aggtcaaggg cttggtactg ttatgactca agtagtttgt gaaactgcta gcctacttcc
     9721 tgaaaatata gtgcatgaag ttccagatac tcatttaaca ccagactctg gtaatactac
     9781 ggcttcaaga cagacagtat ttacgggaga agctactaag gttgcatcaa ataagttaaa
     9841 agaagcttta gactctggca aaactttact tgatttagag ggagaagagt tttatggaga
     9901 atatgaaagt attacagata aaatgggttg tgacaaagaa aatccagtaa gtcatgttgc
     9961 ttatggatat gcaacacaag ttgtaattct ggatgaaaat ggaaaactag aaaaagtagt
    10021 agcagcacat gatgttggaa gagctataaa tccaatcagt ttagaaggtc aaatagaagg
    10081 tggagtagtt atgggtcttg gatatgctct aaccgaagat tacccagtag ttaactctgt
    10141 tcctacagct aagtttggta cattaggttt atttaaatct acagatatac cagaaataca
    10201 agctttaata gttgaaaaga acaaagattt attggcttat ggggcaaaag gtgtaggaga
    10261 aatttgtaca gttcctactg caccagcagt acaaaatgcc tattatgtat ttgataaaga
    10321 atttagaact gagctaccac tacaaaatac tccatatagt aaaaaaaagt aacaaaaaaa
    10381 agaaatatta aataaaaaaa tagatgaaat tgtaaataaa aaaaaccctt ttgcataagg
    10441 tatattttaa atgatagaat atacatatac aggagggttt ttcttttata aaataagttt
    10501 aataaaattg agaggtgagc agaatgaatt ttaataatag tctagaatta actcagtctc
    10561 agaagctaat aatgactaca caacttaagc aatcattatc aatattaaat atgagtaagt
    10621 tagagttaga agaagagata aaaagagaag cagagaataa cccattagtt gaagtcgaga
    10681 aaagtgggga gataaattgg gaagagtata taaaagacat ggataggtca agacgtttag
    10741 atagaacaga gataagctat aatcctgata atgaagttaa tctagaaaat ttagttaaat
    10801 attcgtcaaa tttatatgaa gatttaaaat ttcagataag cctgtataaa cttacagaca
    10861 aagaattgga agtctgtgag tatataattg atagtttaga tgaggatgga tatttaagaa
    10921 ctgaggaaaa agtaattgtt gataccttaa agatagatga agaacttttt gaaaaatgtt
    10981 taattagtgt tcagcagttg gaacctagtg gagtaggggc aagaagtctt tcagaatgtc
    11041 tgatgataca aatggctagt ttaggaatat ataatgaaat tttagaggaa atcgttacaa
    11101 aagacttaaa tttaattgca aataataaat ataaagagat aagtaaaaag tacaatatgt
    11161 cattacaaaa atgtgttgaa ctaataaaca taattaagac attagacccc aaaccaggaa
    11221 gaacttgttc agtagagaaa agtgtataca ttcaacctga tgtaacagta gaaaagattg
    11281 atgatgaatt tattgtatat ttaaatgaga aagattctta ccaaataaga atcaatagtt
    11341 attataaaga catattaaaa aattcacagt ctgatgaaag tgcaaaagaa tttataaaag
    11401 aaagactaaa ttcagcaaca ggacttatga aaaatataga gagtaggaaa actacagtac
    11461 ttaagattgc agaagagata gtaaaagctc aagatgaatt tttgagaaaa gggacaaaat
    11521 atataaaacc tttgaaaatg aaagacatag cagaaaaatt agaatttcat gaatctacta
    11581 taagtagagg tgtaaatggt aaatatatgc taacaccttt tggcgtatat gaatttaggt
    11641 actttttcag tagcgccatt gagactgaga ataatgaaat ggcatctagt acaagcataa
    11701 aaaaaattat aaaggaaaca ataaaagacg aaaataaaaa gaaaccttta agtgatgacc
    11761 atatatctaa gttattaaaa gaaaaaggaa tcaatgttgc aagaaggaca gttgccaaat
    11821 atcgtgaaga attaggaatc ccatcttcaa gtaagagaaa agaatactaa taagtaaaag
    11881 acaaatatca gtatggtaag gggaaaaggc taattaagag atgaatgtag attgggctaa
    11941 aaattttata aagtaaaata atagaatatg gaaaagtatt aaaatgctat aaggtattaa
    12001 aaaactgggg aacattaaaa cattaagata gcaaagaaca taaaaaacta aagaagatta
    12061 aaacattaaa gaacattagg ctttgaggaa tattagaact ttaaagaata ttaaaattta
    12121 taaaatataa aaatact
//
LOCUS       DACTGW010000110        12055 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.110, whole genome shotgun sequence.
ACCESSION   DACTGW010000110 DACTGW010000000
VERSION     DACTGW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12055
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.110"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(384..1787)
                     /gene="hflB"
                     /locus_tag="I9573_002278"
     CDS             complement(384..1787)
                     /gene="hflB"
                     /locus_tag="I9573_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAT4796894.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            2664..3173
                     /locus_tag="I9573_002279"
     CDS             2664..3173
                     /locus_tag="I9573_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAT4796895.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            3201..3395
                     /gene="rpmI"
                     /locus_tag="I9573_002280"
     CDS             3201..3395
                     /gene="rpmI"
                     /locus_tag="I9573_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005211352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAT4796896.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            3451..3807
                     /gene="rplT"
                     /locus_tag="I9573_002281"
     CDS             3451..3807
                     /gene="rplT"
                     /locus_tag="I9573_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAT4796897.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            4117..4662
                     /locus_tag="I9573_002282"
     CDS             4117..4662
                     /locus_tag="I9573_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796898.1"
                     /translation="MNEKLMDCFVNPIKCKLLLEVFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKEGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            4649..4822
                     /locus_tag="I9573_002283"
     CDS             4649..4822
                     /locus_tag="I9573_002283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796899.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLHGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            5098..7023
                     /locus_tag="I9573_002284"
     CDS             5098..7023
                     /locus_tag="I9573_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide phosphodiesterase"
                     /protein_id="HAT4796900.1"
                     /translation="MKIKFVIMPLVIFLIVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFISNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            7424..9328
                     /locus_tag="I9573_002285"
     CDS             7424..9328
                     /locus_tag="I9573_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide phosphodiesterase"
                     /protein_id="HAT4796901.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFKQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEGKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
     gene            9716..10495
                     /locus_tag="I9573_002286"
     CDS             9716..10495
                     /locus_tag="I9573_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HAT4796902.1"
                     /translation="MNYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKVFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVVLGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(10805..11626)
                     /locus_tag="I9573_002287"
     CDS             complement(10805..11626)
                     /locus_tag="I9573_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HAT4796903.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDITVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLEDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
BASE COUNT         4755 a         1358 c         2003 g         3939 t
ORIGIN      
        1 aagtgataga tacctaatta agttgtagat atctgataag aaatttttaa gttttcaatc
       61 gttctaaatt gtgagtataa aaatctacta aatttagtta tgtaaaatat aaattataag
      121 tgtagaaact aggctactag cttttatacc acttcaaaca ttactggaag tagttaagtt
      181 tacagcagtt tagttttatg tagatataat attctgtaaa tagacaaaat taaaatatgt
      241 aattaaaata tgtaagtgtc tcaaaatgaa atttttaatt tatgaggcac ttttttgtat
      301 aaaatcaaat atatttttat aactttaaca ataaaaagag gcttatctct attttgagac
      361 agcctcaaat ttttaaaact atattagttt acagcttctt ttcctatcaa atcttttaac
      421 tcttcatgag tcatagtttc tttttcaaat aagaatttag aaacagaatg taaatcttca
      481 agattatcct ttaacatctc taaagtctct ttataacaaa tttcaactat acgatttgct
      541 tccttttgaa ccttatcaca taagaaagtc ttatcatttc catcaatagt cataaatcct
      601 aagttactca taccatattc acaaaccatt tggttagcta tttcagtgac tttctttaag
      661 tcgtcctttg caccagtaga tttatgatta aatattaatt cttctgaagc ctttccagca
      721 agtaatattt ttatttttcc aatcaactca tccttagtat aaatatatct atcttcatca
      781 ggtaattgaa gaacataacc taatgcttgt cctctaggaa ctatagatat tttttgtata
      841 ggacaaattc ctactatatc acttacaaga gcatgtccag cttcatgata tgctactatt
      901 tttttctctt tttcaaccaa tgcagagttt ttagattcta gccctgcaat aactctttca
      961 agtgccttgt caaaatgttc tgatgtaata cactcactgt catctctaac tgcaaatata
     1021 gctgcttcat ttgctatatt tgaaagatga gccccattga aaccatgagt ttttttagct
     1081 aaaagttcta gatttacaga tttgtctata ggtttgtcat ctgtatgtac ctttagaatt
     1141 tcaaatctag tgtgatagtt tggtgcacct atatgaatgt gtctgtcaaa tcttcctggt
     1201 ctaagtaaag cttcatctaa taagtcaagt ctattggtag cacctattat taatacattg
     1261 gaatctttat taaatccatc catttcaact aaaagttggt ttagtgtttg gtctttttcg
     1321 ttgttacttt caagatgtct ctttgctcct acagcatcta tttcgtctat aaatattata
     1381 cttggagctt cttttctagc tttttcaaat agagttctaa ctctttttgc tcccacacct
     1441 acatattttt caacgaactc tgaaccagta acattgaaaa atgatgagtt tgtttcacca
     1501 gctacagcag atgcaagtag ggttttacct gttcctggag gaccataaaa taatactcct
     1561 ttaggtattt ttgcacccat tttttgatat ttttgtggtg acttcataaa atcgactatt
     1621 tcaaatagtt cttcttttac ttcgtctaat ccagcaacgt ctctaaatgt agtctttggt
     1681 tttgtagaaa gtgaatcatc tgtttgatta tctctttcca tttttgtctg tgcaaagaca
     1741 ggagctggat tgttgatttt cttaaaaaag ttgtttagca ttttcatatt cacacctcag
     1801 gtaattaatt attgttaatc aattttatat tttccatatt tactattttt tattatttat
     1861 ttttcatgga aaaaatatac atgttatcta aataaaaata aatttgtaag atgtatgaaa
     1921 aaatgaattt atgtatatag agatttctac ttatgtattg aataaagtat tacaaattat
     1981 aaattatgta ttgattatat attgagtaaa gtattataaa gcatgtattg attaagtatt
     2041 tattaagtat tgaataaaat attacaaatt tagttgaaat gaaaatgata aaaaatgttg
     2101 tgctatattt tacaaatcat ataaatagag aaattgttgt agcacaaagt aataggtagt
     2161 atattagaat ataatatagg atttgttgtg tagtatagta gcatattaga atataatata
     2221 ggatttgtta tgtagtatag tagcatatta gaatataata taggatttgt tatgtatata
     2281 gtagcatatt agaatataat gtaggatttg ttatgtagaa tatatagagt catgtagaat
     2341 ataaatatat aaaaggattt tgggtataaa tttaaaaatt aatagcaaaa tgagtagtat
     2401 agacatataa ttagtagtag gaagtaatta aatttttcct aaacaaaaaa aatagttgca
     2461 aataatttga ttaagtgata tagtaaataa ggtaagaata aaacaaagaa gaagtccgct
     2521 tctcacctta cgccggaggt tagtaaggta aatataagtt aaagcttaat tttaatttaa
     2581 gtttgtttaa atttggtaac ttatattgcg gatgattacg tcatccgctt tttattttat
     2641 aaaaattttg gaggtgtcct acaattagca aagaattagc aatcaatgaa caaataaaag
     2701 ataaggaaat aagagtcctt tcaccaacag gtgaacaact tggagtaatg cctactaaag
     2761 aagctcaagc aatggctaat agcaaaaact tagatttggt tcaaatctcg ccgaatgcga
     2821 atccaccagt atgtaagata atggattatg gaaagtttag atatgagcaa gcgagaaaag
     2881 agaaagaagc taagaaaaaa caaaaaacta tagttgtaaa agaagtaaga cttagaccag
     2941 gtattgaaca aaatgattta aatacgaaag ctaataatgc tataaagttc cttaaaaaag
     3001 gggataaagt caaagttgaa cttagattta gaggaagaga attaggacat aaagacatag
     3061 gtaaagaagt tatgcttaag tttttagata taataaaaga gtttggagaa ccaacaaaag
     3121 cccctgcatt tgagggtaat aatatggttg taattataga tccaaaaaaa tagatgatta
     3181 actgagagga ggaatttgtc atgcctaaaa tgaaaactca tagaggtgca gcaaagagat
     3241 taaagaaaac aggaactgga aagttaaaga gagctaaagc ttttaaaaaa catatattaa
     3301 ctaaaaaatc tgcaaaaact aaaatgaact taagaaaatc aactttagta agtgatggag
     3361 atgccaagag aatagcacaa ttattaccat actaagatac agattgtaaa taataactat
     3421 agaagaggga tataaaagga ggtcataacg atggcaagag ttaaaaaagc tatgaacgct
     3481 cgtaaaaagc ataagaaaat attaaaactt gcaaaaggat tcagaggatc tagaagcaaa
     3541 ttatatagac cagcaaatac attcgtaatg aaagcattaa aaaatgctta tataggaaga
     3601 aagttaaaga agagagactt cagaaaactt tggatacaaa gaataaatgc agctgctaga
     3661 atgaatggaa tatcttattc aagattaatg aatggattaa aattatctgg tattgaagtt
     3721 aacagaaaaa tgttatctga aatggctata caagacccag aaggatttgc aaaattagca
     3781 gaagttgcta aagcaaaatt agcttagtat tgaatcatga gatgtaaagt ctttgagtta
     3841 tataaataat tcaaggactt ttttattttt tgaaaaatac ttattagtgt catgtacaac
     3901 caattttata atgataaata aaataagcaa tacaatagaa ttttatgaga tttattataa
     3961 gaattgttac aattatttat catagctata aaaagtcata gtaaaaatga tattgatgta
     4021 tgtttatata tgtataaagc tttcaatcaa tcttgaatta atattatcaa catgataata
     4081 ttatcttatt aataatataa ataaggggga gataatatga atgagaaact aatggattgt
     4141 tttgtaaacc caattaaatg caagttattg cttgaagttt tttcaaataa tcgaactaca
     4201 gctaaacaat taggagaaaa atttcaagat atatcaaagg caacacttta tcgttacctt
     4261 aataaaatgt tgaaagatga tattataaaa gtggttgaag aaaataaaat aaggggaact
     4321 gttgaaaaag tatatgctat gaagttagat atagctgatg atgtgaacaa aattatagat
     4381 gagaactctg gagaagaata tgctcaactg tttactcaat atatgatggg atttttaaga
     4441 gaattttatg aatacgctga aagaaaggaa aaggatatcc taaaagaggg ttcaggattt
     4501 tctgtctgtc cagtatatgc tagtattgat gaaatagttg aagtgagtaa aaaaattgct
     4561 agtatactag aacctctaag aaataactcg cacgaagaag gcagaagaat gcattctata
     4621 ggagtgataa tcactccacc taaagaggat gaagggaaat aaatctgatt tacatgaggt
     4681 agagccaatt tacacggaaa atcgttttat gatttacata tgtatccaga gcaaagtatc
     4741 tctgcatgga ttgtacttca ttgtaccatt tcatccaatg acttggagtg gaacaatgaa
     4801 gttttttaat ttttcatatt aatgcctaaa gaaatgaact ttagaccaca tatgcaatta
     4861 ggctacatta tacaattaga ccagatatac agaataaaaa ttagtatttt tatagcagat
     4921 aaattgactg ttattcttct taaaaatcat ctattagtta ttgttagcat attgaaagaa
     4981 attatagtaa tccattgaat aaatgaatat tgcccataat catataaact gctatagttt
     5041 aaaataaagc aggacgttaa attttatagt aatcttaaaa tttaagaggg ggattttatg
     5101 aaaataaaat ttgttattat gccattggtg atatttttaa tagtattatt tgtggggtgt
     5161 caaagtacaa aagatacaga agttaaagca aaagatacag aaattaaaat attaggtaca
     5221 ggtgatttgc attcaatttt gacagactct atggtttcat atgtaaatga ggaaagggaa
     5281 aagaataaaa atctgcttat ggtagatgcg ggagatttct atggtacaca aagtagagaa
     5341 atgtgggaat ggtcttctgg aaaaaagtta attaatatta aaagaaatgg tagagcagag
     5401 tatgatgaca taattaaaag tagtaaagat gaagtaccta tagttaaaga tatggcaaag
     5461 ttgaaatatg atgctgtagt tttgggagat aatgaattta tttcaaatga taagcaaagt
     5521 cttgataaac tagtttctga ttttaaaaat aataatatgc cattggtatc agcaaatata
     5581 tatgaacaat ctggagaaaa ttatgtacaa ccatatgtaa tgaaaaatat taagacagat
     5641 gagggcaatg taaaagttgg aatactaggt cttactataa aagaagtagg ggaaaccctt
     5701 ggtcttgagg attttgaaaa ggataaaaaa gctagagagc ttggagaaca agctgattac
     5761 aaaggaaaat tatatgcaaa tgatttagta gaagatgcaa agaaatggat aaaagttatg
     5821 gaaaaagaaa gtccagacat aatagttgca gttgtacact caggggaaga acctaaaaca
     5881 gatagaaatc caggaaatag aataaaagag ttagcaacta cagtggatgg aatagatgca
     5941 attgtagctg gtcatactca tgaaaaaata gatgaacaca aatataaaaa taaatctgga
     6001 gatgaagtaa ttgttacaca accaggttgg catggagata gagtatcaga gattaatttc
     6061 aaattaaata aagagaatgg aaagtgggac ataaaggaaa agtctagtaa attaaaaata
     6121 attgataaag aagtaagtat agttgctact gcagatttac attcacattt ctctgataaa
     6181 ttaacagttg accttgttaa tgaacgtagt aacccaattg aacctgttgt agtagatgca
     6241 ggcgacttct tagaccctca aacagatgaa atgactcaat ggtataaaga gtggaaaagt
     6301 ataagagata ataatagtga taaaacgatt agtagatgcc caatggtcat ggatatgaat
     6361 cagggaatgt atgatgcagt agtacttgga aatcatgaat ttgtatctga aaaagatgaa
     6421 ttttggtcag atgaacgcct tttagatgct gttatagagg attttgaaca ggtggcaatt
     6481 tctgtgttat cagcaaatat atataggcaa tctggtaaaa attatgttaa accatatatt
     6541 ataaaagatg tggagacgag tgaaggtaat gtaaaagtag gaatattggg acttactata
     6601 aaagaagtag gtaaaggtct taaaaatgta aacttacaag agcaatttca atataaggat
     6661 aaattgtatg caaatgattt agtggaagat gcaaagaaat gggtaaaagt tatgaaaaag
     6721 aaaaaaccag atataatagt tgcagttgta cattcaggag aagaaccaaa aaatccaaaa
     6781 cacccaggaa atagaataaa agagctagca actacagtag atggtattga tgcaattgtt
     6841 gctagtcata ctcatgaaaa aatagatgag catgaatata aaaataagtc tggagaaaaa
     6901 gttatagtga cacaaccagg tgagcatggt aaatattatt ctaaaattac ctttaaagta
     6961 aataaagaaa agggttcgtg gattattaat gataaattca gtaaaacaat tgaggttgaa
     7021 taactttata aaatttatag ataaagtaaa ttatctgata ttgacatgat atataagata
     7081 aagtttctaa tattagtata atgcataatg tgaaatattt taataataag agaataaaca
     7141 agacaaataa aaatatttta acatgtaaga tatagtatga actattatac taatgcttac
     7201 tttataattt gaacatgtta gaataagtct tatttgaaaa ttttagtgta ttagagtaag
     7261 tattatttta ccagtaaaaa atattgagaa ctatatgtag atttgcttaa aaaggaattt
     7321 acatatagtt cttttcgtat aataaatagt acaatcttat atggtaaaga taaagtgtga
     7381 tattaaattt tattatcatc caaaatctaa ggaggagtct cttatgaaaa taaaatttat
     7441 tatagtagca tcaataatat ttttaatagt attacttgtg gggtgtcaaa gtactaaaga
     7501 cacagaaatc aatgtactcg ccacaagtga tttacattca attattccag ataatttaat
     7561 ttcctatgta gaagaggaga gaaaatatga tgagaatcta cttatggtag atgcaggaga
     7621 tttttttgac atgcaaagtg gtgagatgaa taagtggttt actggtcaaa aattagtaag
     7681 atttaaagat gggataccag agtacaaaaa aatagctgaa ccacgtgaag gagaagttcc
     7741 attggctaaa aaaatggcaa agctgaagta tgatgcagtt acatttggga atcatgaatt
     7801 tgtatctaat gacaagcaga gccttgataa attggtttct gattttaaaa ataacaatat
     7861 accattagta tcagcaaacg tatatgaaca atctggagaa aactacgtaa agccatatgt
     7921 aatgaaaaat ataaatactg acaagggaac tgtaaaggtt ggtatacttg gtctgactat
     7981 aaaagaagta ggggaacgtt caaggtttaa aagttttaaa caagataaaa aggctagaga
     8041 acttggggaa caacctcaat acaaagggaa attatatgca aatgatttag tagaagatgc
     8101 aaataagtgg gtaaaggtta tggaaaaaaa agaaaagcca gatataatag ttgccattgt
     8161 acattcaggt gaagagccaa aaaatcctaa aaatccagga aatagaataa aagaactagc
     8221 aacaactgta gagggaatag atgctattgt agcaggtcat acacatgaaa aaatagaaca
     8281 gcatacatat aaaaataatt ctggtgaaga agtaatagta acacagcctg gagctcatgg
     8341 taatagtata tcaaagatta attttaagtt ggataagaag aatgataagt ggtttataaa
     8401 agacaaatat agtgaattaa aggtttttgg aaaggaagta aatataatta caacttctgg
     8461 tctgaatcca aaattttcag atgaaatggc agttaatttg tttaatgaaa gagatagaga
     8521 tgttcaacct atctttatag atactggaga ttttttaaat actaatacaa aagaaatgac
     8581 taagtgggct aaagagtggc aatacaatga aaataaaggc atctataaaa aaataaatga
     8641 atggccaatt gtatatcttg gttatgatgc tgtagtactt ggtagccatg aatttacaga
     8701 ggataaagag acttttgagg gaaaaaaatc tacattggat tatattatag aaaactttga
     8761 agaaatgaag ataccagtat tatcagctaa tatatatgat gaatctggag aaaattatgt
     8821 aaaaccttat ataattcata atgtagaaac tagggaagga aatataaagg ttggagtatt
     8881 aggtcttact actactgata aggatgaaga agacttaagt agctcaagct taagcaaaga
     8941 ctcaaagact tcgaaaattc ctgaagaaac aggaaagtta tatactaata atctagtaaa
     9001 agatgcaagc gaatgggtaa aagttatgca aaaggaaagt cctgatgtga ttgttgcagt
     9061 tgtacattca gataataata gtactctaaa aaatccaagt agtgaagtca aaaaacttgc
     9121 aatgtcagta gatggaatag atgctattgt tgtaggtcat acgaaaaata aggtagaaga
     9181 acatatttac aaaaataagt ctggagatga ggttatagta acacaatctg gagaagattg
     9241 ttattcaaaa ataagtcttg aactaagaaa agaaaatggc aagtggaata tcataaacaa
     9301 atatagtaaa gcaataaaaa tgaagtgaat ttattgatat ataaaccatt attaacactg
     9361 atttttataa tataataaat actgattcta caatataatt aacactactt ttttaaatat
     9421 aatcaatact gattctacaa tattattaat actacttttt ataatataat caatactact
     9481 ttttacaata taatcaatac tgattctata atataattaa cactactttt taaatataat
     9541 caatactgat tctataatat taacactatt ttttatgcta tataacaatt attgaaaatg
     9601 atataaatat aatatctcaa atgatatgaa tataacatct gaatagaggg tgttttattt
     9661 gtaaaaaata agtttacagt ttgagcgtat aaacttatgg taaaggagaa taattatgaa
     9721 ttataagaag attgcaatta taacgggtgc taatagtggt tttggtaaag agtttctaaa
     9781 gttattaata aatgaagaag aaataacaga aatatgggca gttgcaagaa acaaggcgag
     9841 attaaatcaa ttagtagatg aatttggaag taaggtaaaa gttttttcaa aagatttatc
     9901 aaaaatagaa gaagtaaaag aaattggtac atttttaagt aaagaaaatg tatgtataaa
     9961 ataccttata aacaatgctg gttttgcaaa attctgttca tacaatgatt taagtataga
    10021 tgaatctatt aatatgattg atttaaatat tagtgctgtt gtagtattgg gacttatatg
    10081 tattccatat atgcaaaagg gaagccatat tatgaatatt tcttcacaag catcattcca
    10141 gccattgcca tatcaaaata tttatagttc aactaaagcg tttataagaa attatacaag
    10201 agcattaaat gttgaactta agagtaaagg gataaatgca attgctgtat gtccaggttg
    10261 gatgagtaca aatctattta agagaggtat agttgtagct gaaaaaggga caaaaaattt
    10321 cccaggcata gtgtctccag acgttgttgc caaaaaagca ttgaaggatg caaagaaaaa
    10381 taaggatatt tctgtgtatg gtataaatac aaaatccagt catttattag caaaactatt
    10441 gccacaaaaa atgatgatga aggtttggtt aatgcaacaa catattaagg aataagagtt
    10501 tcggtaatta agtatcagtt ggagattcaa tctattatat attataagaa gttttgttga
    10561 gcaaataata attgtgacaa aacggcttat ctagtgccga taagccgtct ataatgtata
    10621 aaaaatatat ttttgatttt actttattta gatattgaag tattaaaata tgtaggactt
    10681 gaaattttat aaagataaat actatgatat tttgtaaaaa ctgagatatg ttgatattac
    10741 atagtttaac tatactgtta aattgaagtt ttcttatttt gcattatgct tattacttaa
    10801 tgatttagac tataatttcc ataccatcat atgcaaaata aatgttatca agtttctttt
    10861 ctaattcact gtaataacca tatgattttc cccacatttc gtcaatatgt gtaatgataa
    10921 tctttttaat tctataatag tttttaattt caacaatctc atctaaagta aatatttcat
    10981 ctttaaatgg tgtgtcttca agttttgtac catcatttaa tataccatca tcagaaacta
    11041 gacttattat taaaacatca gcatcaaagt acatatcact atgagtaaat ggcttgggat
    11101 tacagcaggc atatatgact tttttttcat tctctttgat aacatagaag gttatattcc
    11161 tgtctgggtt attgttaatc aaatcaatat caaaattatt tatctttaca tgcgaagttt
    11221 cattaataga aatgcaattt ataacatcac cataatattt aagacaatcg agattcgttc
    11281 ggtttatatc ttcaattact tcaggaagag cataaaagcc aattggatta ttttctcctg
    11341 tgataaagtc acatcctatt ttttcgacaa ttctaatacc cttaacatgg tctgggtcat
    11401 ggtgagaaat tgaaatatgt tttacttccg atattttttg tctgttacaa gctactgtaa
    11461 tatcttcagg tgtgtctatc agcattttta tatcatcaat atataaactt ggtcctaatc
    11521 gttcatatct tccgcctttt tgcctggctt cttcacatat tttgcattta caaaatgaac
    11581 ttggaataat tgacataccg ccactaccaa caatttttaa tttcaaagtt attacaccac
    11641 cttctaaaaa tattagatta tataaataat ctacaatcac aactgattaa caaaattatg
    11701 agttaataaa ttatatcaca tgtaaatatg aaagaatagt atgaaatttt tataagatta
    11761 tattaaatat ttctcaatga aattgacttt gtatatgagc cgaatgactt tattcaatat
    11821 agataagaca tttttcttgt aataagatat gattattcta taaggttcat gtaattttga
    11881 tataaaatat tgattaataa catatatgtt attggtgaac atatttctat agaatggaat
    11941 atataatttt ttatatttat cattgtatta ttatacaaag aaagttaaaa agagtagagt
    12001 acaaaggaat tatttgtact ttactctttg ttttgttaaa aactaattta tatta
//
LOCUS       DACTGW010000111        11923 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.111, whole genome shotgun sequence.
ACCESSION   DACTGW010000111 DACTGW010000000
VERSION     DACTGW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.111"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            77..778
                     /locus_tag="I9573_002288"
     CDS             77..778
                     /locus_tag="I9573_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HAT4796904.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIINIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1049..1867
                     /gene="larE"
                     /locus_tag="I9573_002289"
     CDS             1049..1867
                     /gene="larE"
                     /locus_tag="I9573_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HAT4796905.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNMDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKILGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLFNLGFSQFRVRM
                     HGEIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            1922..3070
                     /locus_tag="I9573_002290"
     CDS             1922..3070
                     /locus_tag="I9573_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAT4796906.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3206..4594
                     /locus_tag="I9573_002291"
     CDS             3206..4594
                     /locus_tag="I9573_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796907.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYTTLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4623..5321
                     /locus_tag="I9573_002292"
     CDS             4623..5321
                     /locus_tag="I9573_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4796908.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     ENDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            5324..6757
                     /locus_tag="I9573_002293"
     CDS             5324..6757
                     /locus_tag="I9573_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4796909.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            6779..7516
                     /gene="truA"
                     /locus_tag="I9573_002294"
     CDS             6779..7516
                     /gene="truA"
                     /locus_tag="I9573_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAT4796910.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     gene            8087..9286
                     /locus_tag="I9573_002295"
     CDS             8087..9286
                     /locus_tag="I9573_002295"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAT4796911.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            9533..10579
                     /locus_tag="I9573_002296"
     CDS             9533..10579
                     /locus_tag="I9573_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HAT4796912.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DGGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDERIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPSKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            10687..11850
                     /locus_tag="I9573_002297"
     CDS             10687..11850
                     /locus_tag="I9573_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT4796913.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKIIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SKNPSSTDLQEIAKEITDISKKDYEIFSMYTGDDNWYYMIQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
BASE COUNT         4876 a         1123 c         2147 g         3777 t
ORIGIN      
        1 taatagtggt tttaaaaatc tgacaacaaa aataaaactt tcaaaaaatt aaatctaaaa
       61 agaataggag ataaatatga gtattgaaga tttatatgat tttgaatgtg ttcctttgaa
      121 caatgagatt gatattgatg ttttatttga aaagatgata gaagatggaa aagaaaaaga
      181 atatacacca attatagtca ttgatgaaaa agtagggcta ttaagaaaaa atatagatat
      241 gattactaaa gagtatggtt ctcttgaaaa ctatagagag tattgtttaa caaaatataa
      301 tgaaattgat gtaaatcaat tttttgattt taggaagagt gagatttcag aattattaaa
      361 ggagctaatt gatttagaag atgattttta tgattataaa cattttagac caattataaa
      421 tatagatata tttcatgaat ataacaaagt ttacatagct aaaattccaa caattaaacc
      481 atatgaagta tttgcataca tacctattgg aggatttaat gattgtccta gagatgaaga
      541 acatatagct attgcaaaat attggtatga aaagcatgga atctttccaa tcgcaattgg
      601 atgtgataca gttcaatatt ctgtaaaaaa tttaactaaa gatggtaaaa aatttgatga
      661 cttatgtatt gaattagtat tttattgtga ggatattatt atacaagggt atgaaacctt
      721 aaaggctctt aaggatgtat taaaacgctc tacaatagtg cttttttggt gggattgata
      781 ttctatcatt tagatagtat taaattgtta gtcaccaaga atccacttac tttagttatg
      841 gggagtgtgt caaactaggt ttttatacac agtgtagcat agtaatatgt ttaaattaca
      901 aaggttaaaa aaatacttta atatttttaa tattataatt aatagattta aaggtaatta
      961 taatttataa aataaatact ttatatgtta taatcattaa gaatgtaaaa aaataatata
     1021 tagttttata gttcatattt ttccagatat ggaggtaaat tttgtgaagg aaaaagaaaa
     1081 attagataaa ttaaaaaaaa tgttacttga attaggcagt gttgtagttg cttattctgg
     1141 tggagtagat agtaattttt tgcttaaggt cgcaaaagat acattaggtg aaaatgttat
     1201 agctgtgacc attcatgcaa tgatgcattc aagtagggaa attgaagagg ctaaacaata
     1261 tactcaaaat tttggcgtta aacatatcat attaaatata gaaaactttg atttaaaaga
     1321 atttaaagaa aatggtgttg atagatgtta tcattgtaaa aaatatatat tctctaaaat
     1381 aaaagaggta gcaaaagaac ataatataaa gtacatagta gatgggacaa atatggatga
     1441 tttaggagac tatagacctg ggcttaaggc attgagtgaa ttaggagtga ttagtccatt
     1501 aaaagatagt agtttaaaaa aagaagaaat taggtcgctt tcaaaaatat taggattaaa
     1561 tacttttaat aaaccatcat ttgcatgtct tgcaagtagg attccttatg gagttgagat
     1621 aacagatgaa aatttaagaa tagtagaaaa gagtgaagaa tatctattca atttaggttt
     1681 ttctcaattt agagtgagaa tgcatggaga gatagctaga atagaagttg ggcaggaaga
     1741 actaggtaaa ttttttgaaa ataacaattt taataaagtt gatactaaat taaaaatatt
     1801 tggctttaaa tatgttacac tagatatgtc aggatataaa atgggaagta tgaatttaaa
     1861 tgtgtagatt attttaaaaa taaataagtt ttatagttaa tataaggaaa caggaggttt
     1921 tatgaaaaat tatacattaa tatcgccatg tttttttgga atggaaaaaa tgctggctag
     1981 agagattact aatttaggat atgaaataat aaaaacagaa gatggaagaa taacatataa
     2041 aactgatgag tttggaatag caaagtcaaa catgtggttg agatgtgctg aaagagtaca
     2101 tcttaaaatt gctgaatttg aggcaaaaag ctttgatgaa ttatttgaaa atacaaaaag
     2161 aataaactgg tctagatata taccttatgg agctcaattt cctatatcaa aagcttcctc
     2221 tataaaatca aaattataca gtacaccaga tgtacaagcc atagtaaaaa aggctatagt
     2281 agaaagctta aagaaaagtt atttagagga tggtttgctt aaagaagata aggagaaata
     2341 ccctattttt gtatttatac acaaagataa agtaaccatt tctatagata caacaggaga
     2401 tgctttgcat aagagaggat atagagaaaa ggcaaataaa gctccaataa gagagacttt
     2461 agctgctggg cttatttatt taactccctg gaaagcaggt agagttttag tagaccctat
     2521 gtgtggttct ggtactatat tgattgaagc tgctatgatt ggtataaata tggcacctgg
     2581 cttaaataga gagtttatat cagaaaagtg gagaactctt gataagaaaa tatggtggga
     2641 tgtaagaaaa gatgctttta ataagataga taatgaatct aaatttaaga tttatggata
     2701 tgatatagat gaagaatcta tagatatagc tagagaaaat gctgaaatag caggtgtaga
     2761 tgaatatata gagtttaatg taggagatgc aactcaattt aaaagtgaag atgagtttgg
     2821 atttataatc actaatcctc cttacggaga aagattagaa gataaagaca gtgttaaaca
     2881 gttgtataag gagcttggtt atgcatttag aaaattaaaa aattggtcgt attatttgat
     2941 aacttcttat gaagactttg aatatgagtt tgggcaaaaa gcagacaaaa aaagaaaatt
     3001 gtataatggt atgttaaaaa ctaacttttt ccaatatccg ggtcctaagc ctcctagaaa
     3061 taataaataa gttttacatg atttatttgt aaaatataaa attttattaa aataagtaaa
     3121 acattatgat gccttgaaac gtctaattat tgtgaggaga tttttgtgaa caaatttaaa
     3181 gatttatagg attaggagga gttaaatgaa agtgttaaag cttattactg tagtagccct
     3241 gatgatgtta tttgttactg gatgtaatat aaaaactgaa tctccagaac aattagcgaa
     3301 gatacctgac tatgatgata caaaaaaagt attgtatgat ggtattgacc agttactaaa
     3361 accagattca tctatgatat tgccttctaa tacaaaagaa gtaggaaaaa taaataaggt
     3421 agatttaaac agcgatggaa ttgatgaatt agtagtattt gaacaaaaag aagatttaag
     3481 taataatgtg agtcaggtag ggtttgtaac cttgagttac aatggagaaa aatacgtact
     3541 tggagaccat tttttagaaa atggcgaatc aatagaatat gctaattttt atgatttgga
     3601 ctcagatgga tataaggaag ttatcttatt ggttaaatcc aaagacaaaa cgaatatgca
     3661 tatatataaa gtagtagata atgaaataac aaaaatatat gacttggatg catcatggct
     3721 tcaaaataaa gaagatttta atgacatgaa agtaaaaatt ggatatatag atggagatga
     3781 caagttagac atattgatgc ttcatttgaa taataagact aatgaaatgt ttgcaagtgt
     3841 tgcaaatttt gatgggaaaa tgaaaatcaa agattctgtt aagtttgaaa atgtaaaaaa
     3901 cttaagtgaa ttatatacaa cattaggtaa tgttgcttca tctgttggaa attcttctgc
     3961 agctataaaa ggtattgtac ttgatattcc tatattgaaa gataataact atattacgca
     4021 gatactttat atgaaagatg ggaaaattaa caaagctttt agtgattatg ataaaactat
     4081 tactaaatct tattatatac ctgtagatga tattgacaaa gataagatta tagaaatacc
     4141 tatagttgca ggcagtacag gtaataataa aaatacttat agttctaaat catctgcaac
     4201 tataagttgg tatagatgga atggaaaaga aggtgctgac tcaagtttaa tatttacaag
     4261 tcagatatat tataattata aatataattt taaattattt attccaaata atttatttga
     4321 taaaatactt gttgaacagg aatttgttgg agaaaaagca gtatttaaat tttactattt
     4381 tgactattca gatcgtaaga atttatttaa tatagtagta gagagtaaaa acaaacttga
     4441 agatagaaaa aatacaggta ctcaaaactc tattgtactt caagagagcg atgaatatac
     4501 tttcttacta gttgtaaaca atgcaaatga aatggataag ctagatatga ctatagacgc
     4561 attaaaagaa tatttctcat taatatatgg ttaatattat actcaattgg aggttttatt
     4621 ttatgaagga aaagatactt atactagaag atgaaattgg tattagaagt tttgttagta
     4681 taaacttaaa aagagaaggc tatgaaatag ttgaggcagg tactggaaga gaagctatag
     4741 aaaaaatgac aacagaaaat gatattacaa tagctctttt agatgtgatg cttccagata
     4801 taagtggtat agaagtatgt aaatttataa gggagaattt tgaccaagta ggtataataa
     4861 tgcttactgc taaagctcaa gaggatgata aaatagaagg atttatttca ggagcagatg
     4921 attacatcat aaaaccattt agtataaaag agcttttagt cagggtttct gctcttctta
     4981 gaagagtagc aaaggatgat tcaagtgtta agtcgagtga aatagtttct ccaccattta
     5041 tacttgatat agacaagaga aaattattta aaaatggaaa ggaaattgag cttacaccaa
     5101 ctgaattttc aatagtgaaa tatctaatat caaatgctaa gcaatcgtta agtagagacc
     5161 agatattaga cgaagtatgg ggaactaact atttatatga ttttaaaata gtagatgtaa
     5221 atataagaag gataagaaat aaaatagaag atgacccatc aaagccaaaa tatatacaaa
     5281 ctatttgggg ttatggatac tgttttagaa aggaagaata atagtgttac attttgaagg
     5341 tctgagaaaa aaagtaataa aaaactattt tataataatt attataatgg ttactctgtt
     5401 tgaaggattg ttcatgtttt acatacaaaa ctactattat gattctgtca aacaactact
     5461 tgaatctgaa ataaaatatg ctgatgaata taatgctatt acaatggaaa ctacaagttt
     5521 tgagaaaaaa gtaaaaaata tatttgataa acaaccatta actaaaaatt cagaatttgg
     5581 aatttcaata atagataaag ataaaaatat tatattagac caatatggtt ttaaaagtaa
     5641 agaaaaagca aactatgaag atgttaacaa tgcattaaaa gatataaaaa ctaaaaattt
     5701 aacaccttat acttatagaa taccagatac aggggagcat gttatgagta tctccttacc
     5761 acttaaggta aataatataa tagaaggtgt tgttagatat acagtatcat tagatgctat
     5821 tgataatgca atattaaagc aagctacatg gcttatatta gcaggaatat ttatacttat
     5881 tatagctata ctaataagtc ttaaatttgc tgagactctt ataaaaccat tacgagagct
     5941 aaagaaattt gcaaatgaat tggctgtagg aaattataat ataaaattag aaaagatgaa
     6001 gattgtagat gatgagattg gtgatttagc acagacattt gaacacatgg cacatgagat
     6061 tgacaaaagt gaaaaattaa aagaggaatt tatatcatct gtatctcatg aactaaggac
     6121 tcctcttaca tctataaagg ggtggagtga aaccttaggt tatgaaagta taacaagaga
     6181 agaactagac ttaggtttag gcattataca agatgaaaca gaaagactta taaaactagt
     6241 tgaagaatta ttagactttt caaggctttc ttcagacagg ataaaacttc atgttgatat
     6301 agttgatgtt gaaggtttaa ttgtaggtgt tgtaaatcaa cttaaagtta aagcagctga
     6361 aaaagatata tcacttttat ttgaatttga aaatgaattt atagagaaca ttcaaggaga
     6421 taaaaataga ttaagacagg ttctcataaa tttaatacaa aattcattta aatttacgag
     6481 tcaaggtggt tacataaaag ttgtagcttc tcaagatgaa gagataacaa ccatatcagt
     6541 agaggataat ggttcaggta tagaaaagca aaatttaaat aaagtgttag ataaattttt
     6601 ccaagaagat tataataagg ctggtagtgg attaggatta gcaattagta atgaaattgt
     6661 aaaacttcat ggtggaagaa tgaaaataga aagtgaaaag aatgttggaa caaaaataac
     6721 ttttaatata aaaaataaat ttgctaaaca agcataaatt cttagaaaga ggattaagat
     6781 gagaaacata aaaatgatag tagcatacga tggttcaaga tataaaggat atcaaaaatt
     6841 aggtgacaat aatatgacta ttcaagaaaa acttgaaaat gttttaagta aaatgactaa
     6901 tgaaacagtg gagataatag ggtctggaag aactgatatg ggagctcatg caagaggaca
     6961 agtggtaaat tttagaacta attgtatgga ttctttggat aaaattcaaa aatatttata
     7021 tgaatattta ccagaagata tagttgtaaa aactgttgaa gaagttgatg aaagatttca
     7081 ttcaagatac aatgtaaaat caaagactta tatgtataaa attgacaata ataagtatca
     7141 caatcctttt ataagaaaat atgctactca tgtaagtaaa aaattagatt tagatagaat
     7201 gagaaaagca agtgagtatt tagtaggaga acatgatttt actagttttg cttcttctaa
     7261 atctaagaaa aaatcaaatg taagagaaat ttattctata aatataaaag aagatgataa
     7321 tgttatagaa atatatgtag aaggaaatgg atttttgtac aacatggtaa ggataatagt
     7381 aggtgctttg atagatgttg gtctaaaaag aagagctcca caagatatta agtatatgct
     7441 tgaatcaaaa gatagatgtc aatcatcaga tactgcacca gctaaaggac tatgtctttg
     7501 gaaagtaaca tattagtcaa ttatataaaa ctctctttaa aataaaaata aaataaatta
     7561 tatagttgac ataaatggaa aaataaacta ttatatattt tataagagag atataaaact
     7621 ttatatttaa atcctgtgag aaagagagta acttctatgt gttttataga gagctgagga
     7681 tggtggaagc tcagcaaata agtattagtg aaaagaactt tggaggatac taattgaatt
     7741 tagtatgtta gtaggtaatc tcgccttaag agaaagagtg gataagttta tcttatcaat
     7801 tagagtggta acgcggatat atttcgtctc ttggtttttc caagaggcgt tttttaatat
     7861 atttaattat tagtgtttat ctggtatgtt gaacatttac taattgatta gctaagttta
     7921 tcaataagag tggtaccgcg gatatttcgc ctcttagtat tttaaaatac taaggggttt
     7981 ttttagtatt aaaatgattt tatgtgagtc taaattctca gagtcattca atattaagaa
     8041 tttctataaa ataaaaatta taatttaaga ttgggaggaa ttaaaaatga aggaaaaagt
     8101 agtattagca tattcaggag gacttgacac atcaatcata ataccttggt taaaggaaaa
     8161 ttatgaagat atagatgtta tagctgtttg tggaaatgta ggtcaggaag ataaaatgga
     8221 agatgtatat gaaaaagctt tacaaagtgg agcatcaaag gcatatgtgg atgatataag
     8281 tgaagaattt gtaactgaaa ctatatttaa agccgtaaaa gcagaggcta aatatgaagg
     8341 aaaatactta ttaggaactt ctttagcaag acctataata gctaaaaaat tagttgaagt
     8401 tgctcataaa gaaggtgcaa aatatatatg ccatggatgt acaggaaaag gtaatgacca
     8461 agttagattt gaagctacaa ttgcagctct tgacccgact ataaaagtta ttgcaccttg
     8521 gagaatttgg gatataaaat caagagaaga tgctatagac tatgctgaaa aacataatat
     8581 aaaggtaaca gcaactaaag ctaaaatata ttcagtagat gctaaccttt ggcatgtttc
     8641 aacagaaggt ggagatatag agcacctaga aaatgaacat aaaaaagatg tttataaaca
     8701 atgtgtagac ccagaagatg catgtgatgt agctgaatat gtagaagttt actttgaaaa
     8761 aggtgtacct aaaaaagtaa atggcgaaga attatctcca gttgcactga ttcataagtt
     8821 aaatgagttg ggttgtaaac atggaattgg tgttatagat atagttgaga atagacttgt
     8881 tggtatgaaa tcaagaggta tatatgagac tccaggagga acaatccttt atgaagcaca
     8941 taatatttta gaaagtgcta ctttagacaa agatacatta catttcaaac aaatggtatc
     9001 ttataaatac ggagaactta tttacaatgg actatggtat tgtaaattaa gagaatctat
     9061 agatgcattt atggaacaaa ctcaagataa tgttacagga acagtaaaag ttaaacttta
     9121 caaaggaaat ataaaacctg ctggaatatt tactgaaaat gctctttatg atgaaggaat
     9181 atcttctttt ggaaatagtg aactttatga ccacaaagat gcagaaggat ttattaattt
     9241 attcacttta ccattaaaaa tcagagctat gaaagctgga aaataataaa ataaattatt
     9301 taagagtgta taaaaactat ttttaggttt ttatatgctc tttttttatt tgaatttatt
     9361 aaattataca gtaaatagta taaaaaaata tattatagtt ccaaatgact taataatgtg
     9421 aaaatataag acctaaaata taaattttat aaatacaata gttattgact ctctcattgt
     9481 aagagggttt atactgaatc taatgaaaat ttatagtgag gagatagatt atatgagaaa
     9541 aataattgaa attaaacatc attcacatga ttatgagtgt atgtggaatg gtatagaaga
     9601 tttatatata aataaaaccg gtgaatgcct tccaaacgat tttttctttt tactgtcagg
     9661 ttttggttca ttttgttata tgaaaacaaa taaagctgat ttgaaaagaa tggtagcttt
     9721 gggagatgga aggacaaaac agatgtataa atttttagca ccaattgttg gatttgaata
     9781 caaacatcat gagtttaaaa agtatgagca agcattaaag aaagcaaaat ctgaaattga
     9841 tggtggatat ccagttattt taggagcttt agatatgtat tatctaccat attataagaa
     9901 gctatatcat aaagaacaca ttccttttca ttatatatta atggtaggat atgatgatga
     9961 aagaatatat ctgtatgatt gtggtagaac agaattatta catctttctt atgataaatt
    10021 acgtgaaagc atgaattgca attcccctgg attaagcaaa gaaaatacta tttgcacaat
    10081 aagaatgaat acttcaaaga ataaatataa cattgctaga gaagctattg ctataaagcg
    10141 tgatatgttt ttaaatccat ctaaaggatt tttgggatat aaaggttttg aaaagtttat
    10201 taaagagttg ccacagtgga acactgaatt ggataaagat gattatgata agattttgac
    10261 caatatggtt atgttttttg gaacagtacc aacaattcca aatgcaatca aaggaattaa
    10321 agaaccagat actgttcaat ttaaaggtgg atttgataaa atgagtagaa tgctcaacag
    10381 tattggggag gaatacaaaa acgactcttt tatacaagta gctgatattt ttgataaaac
    10441 tgctattata attgagaaaa tatccaatat tataatagat tatttaactc aaacatgtga
    10501 tgatacagaa caacttccgt tattgttctc tgaggtctta gagcatatga aaacaggata
    10561 tttaatcttg gatgtataaa aaatatattg ttgaccctaa cattacgtaa tagtgtaata
    10621 tgaaaatata gcttgaatat attagtaggt attgatggct gtaaaattaa aatttgaggt
    10681 gtggatatga gaacagtgaa acaagtttct gatttaactg gaataagtgt gagggcatta
    10741 cattattatg atgagatagg tcttttaaaa ccaaataaaa ttacagatgc aggatataga
    10801 ctttatgatg atgaatctat taaaactctt caacaaattt tattttttaa ggagattgat
    10861 attcctttaa gagaggttaa ggaaataatg tcaagccaat attttgataa ggttgaagct
    10921 ctaaaaaatc aaaaaaaatt acttatttta aaacgaaaga gactggatga attgatagag
    10981 cttataaatc aaactctaag aggagaagga aatattgatt ttaaagagtt tgatatgagc
    11041 gagtatttta atgttttaga agaatttaaa agagaacata ggaataagat aattaaaatt
    11101 tatggtagtg tagaaaaata caatgaatat atagaaagag ttaagtctaa tgaagagaaa
    11161 attgctaaaa tggctataaa acaatatgga actattgaga agtttgccca agctataaaa
    11221 acaaatttta gcagtgatat attgaattta ggagaaaaat ttgatagata taaaaatgat
    11281 tgtttaaaag aaaaacatcc aaaattaaaa gaattatata gaaagcttgt agaagattta
    11341 agtaagaatc cttcttcaac agaccttcaa gaaatagcaa aagagataac agatatatct
    11401 aaaaaagatt atgaaatttt ctctatgtat actggtgatg acaactggta ttatatgatt
    11461 caaaattatc tagtaaatcc tatgtggata gaagaagtag ataaaaagta tggaagtgga
    11521 gcaggcaagt ttattggaca agtactaaag acttatctaa gagatagaaa accaaagata
    11581 aatacattgt atgaaaaact tgtagaagat ttgagtagag attgttcttc aagggaagtt
    11641 caaagcattg ttgaagaaat agacaatgaa atgaaaagaa gtaatgaatt ttataaaata
    11701 gacaatggtg aaagatactt tgattatatg tcagagcttt atttacaaga ttctaactat
    11761 ataaaagtaa ctgataagaa ttatggtgat ggtgcatcta agtttattgg agaagctttt
    11821 aagatttatt ttgataataa taattgttga aaatttggaa gtaaaaaata tgtaagtttt
    11881 ataaaagcac aaaaataagg atgtagatta gctccatcta tat
//
LOCUS       DACTGW010000112        11894 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.112, whole genome shotgun sequence.
ACCESSION   DACTGW010000112 DACTGW010000000
VERSION     DACTGW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.112"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            126..1166
                     /locus_tag="I9573_002298"
     CDS             126..1166
                     /locus_tag="I9573_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAT4796914.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1495..2844
                     /locus_tag="I9573_002299"
     CDS             1495..2844
                     /locus_tag="I9573_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAT4796915.1"
                     /translation="MSKYQKNVSMFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVV
                     GGAVGVSPTQITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVG
                     GNYGLASILGAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMA
                     GGVNSPTYASMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIIN
                     FDPIREATWFAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNIT
                     DKELSSGVIGSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSG
                     FVPKFGALMTTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMG
                     ITSVPAAQEAFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3142..4503
                     /locus_tag="I9573_002300"
     CDS             3142..4503
                     /locus_tag="I9573_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine permease"
                     /protein_id="HAT4796916.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4576..5970
                     /locus_tag="I9573_002301"
     CDS             4576..5970
                     /locus_tag="I9573_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4796917.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQLAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6032..6502
                     /locus_tag="I9573_002302"
     CDS             6032..6502
                     /locus_tag="I9573_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HAT4796918.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIKEAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6505..8721
                     /locus_tag="I9573_002303"
     CDS             6505..8721
                     /locus_tag="I9573_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAT4796919.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFRGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLIVEEAEKLLETSKEDLYLDNGYVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGLTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVNSYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8804..9475
                     /locus_tag="I9573_002304"
     CDS             8804..9475
                     /locus_tag="I9573_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT4796920.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9838..11052
                     /gene="dpaL"
                     /locus_tag="I9573_002305"
     CDS             9838..11052
                     /gene="dpaL"
                     /locus_tag="I9573_002305"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HAT4796921.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11179..>11894
                     /locus_tag="I9573_002306"
     CDS             11179..>11894
                     /locus_tag="I9573_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HAT4796922.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPG"
BASE COUNT         4495 a         1371 c         2401 g         3627 t
ORIGIN      
        1 ctttttatta agttttgttt taaaataata ttattaaatt ataactaagc ttttctgtta
       61 tgatataatt aaatagataa tgtgaatgat tattatatag aatacttatt tggagttgat
      121 attttatgga caaacacagt gattcagtaa tgtatggtgg aatggaagta tataatattt
      181 ggggaacaac aacagtagac tccataattg gagagtctga tgtaatgcgt gctcttaaga
      241 atagaatttg caaaattgga aatagtgatt ctactgtagc tataactgga gaaagtggca
      301 ctggaaaaga attaatagca agagccattc atctcgcagg aaacagaggg aataaaacat
      361 tcgttgctat aaactgtgct gctataccag aacctttact agaaagtgaa ctttttggat
      421 atacaaaagg agcttttagt ggagcagatt caaaaggtaa aattggtaag tttgaattgg
      481 ccaatggagg ggttgtattt ttagatgaga taggagatat gccaattcaa ctacaatcaa
      541 agttattgag ggtattacag gaaaagaagt ttacgagaat aggttcaaat gaactaattg
      601 atatagatgt tagaatcata tcagctacta atagagattt atttgagttg gttaaggaaa
      661 ataaatttag agaagactta tactatagat tgaatgtaat acctttagaa gtgcctccac
      721 ttagagaaag aggagacgat atacaaatat taataaataa ttttatagac aaatattcga
      781 gaaaaatgaa gaaaaacgtg taccatatag aaccagatgt ggaacaaatg ttgcttaagt
      841 atccttggcc aggaaatatt agagagttgg aaaatactat agaactggca ttaaaccttc
      901 ttgaggaaga tggaataata cataaaggaa taataaatag aaagatactt gaatatttta
      961 agttaaataa tataaatata aagttacttg aggaaagtga ctatgagata agtatggaac
     1021 aagacatact tacacttgaa gagtttgaaa gagcctatat aaagaaagtg ttaaagtttt
     1081 acggaaatga tactaagtct aaaaaactgg ctgccaaaaa acttggaata tcactagcta
     1141 cattgtacag aaaaatagat gtataataat taggtttttt attctcaatt ctaaaatttg
     1201 aaattataaa attgataaaa aacacttgga aaactatttt ctaagtgttt ttttgtgtaa
     1261 tagcatttta ttatccaaaa ataaaaaaaa tttaaaagaa tttaaaaaaa taaatgcttt
     1321 aataaattat caaatactaa caaatataat aaaaattata tatgtaaaac aattatgtta
     1381 aaaaattggc attataattg cttcatatta aattgagatg acaaaataaa aaaagaaagg
     1441 atgttgtgtg agataataat aactgtcatt aaaaaatagt tgggagtgat aaccttgagt
     1501 aaatatcaaa agaatgtttc tatgtttgat attgatggaa ggccaccaat tttaaagtgt
     1561 ttgccactat caatgcaaca tatactagca atgatagttg gaactgttac agtacctata
     1621 gtcgtaggag gagctgtggg tgttagccct acacaaataa cacttttagt gcaatatgca
     1681 ttaataatag ctggtatatc aacacttata caactatatc caataggaaa tattggttca
     1741 agattgccta taatttttgg agttggtttt acttatgtac caacacttgt agcagtcgga
     1801 ggaaattatg gattagcaag cattttgggt gcacaattaa tagggggagt ggctacagta
     1861 ttaataggga tattcataaa aaagataaga aaattttttc cgaatatagt tgcaggaaca
     1921 gtggttttta caattggatt atctttgtat cctatagcaa taaattatat ggcaggtggt
     1981 gtaaatagtc ctacttatgc ctctatgcaa aactgggcag tagcggctat aacattagtg
     2041 gtagttatag gtcttagtca atttgcaaaa ggatatgcaa aattggcagc aatatttttg
     2101 ggaattatag ttggatattg tgtatcttta gttctgggta taataaattt tgaccctatt
     2161 agagaggcaa cttggtttgc gatacctaaa ccgcttgaat ttgggatgga atttgatatg
     2221 cctgtaataa tctcagtagt aattgtaagt ataatcaatg ctgttcaagc aattggagat
     2281 ttatcagcta ctacaatggg aggcatggat aggaatatca cagataagga attatcaagt
     2341 ggagtaatag gaagtggaat ttgtactatg ataggagctt tgtttggagg tttaccacca
     2401 tcatcatata gccaaaacgt ggggatggta gctatgaaca aagtaataag tagatttgtg
     2461 cttggaatag ctggtatatt tatgttattg tcggggtttg tgcctaaatt tggtgcatta
     2521 atgactacta taccatattc tgttttagga ggagctacaa tatctatatt tagtatgata
     2581 acaatgacag gtataaagtt aattattaca gaagagttgt catcaagaaa tattaccatt
     2641 gttggtttgg caattgcctt aggcatggga ataacatcag taccagcagc ccaagaggca
     2701 tttccttcat ttgtaaagtt agtatttgga gaatctccaa tagtaattgc aacattggtt
     2761 gcattcgttt taaatctaat aataccaaat aaaagtctag aggatgaggc taaagaaaga
     2821 gctgttatgg aagagactaa ttaagatata ataatttaaa agaattgttt aaattattta
     2881 gtatgagatt gttagattat ttttgatagc tattcatttt aataaaaaat ttaacaaata
     2941 aatatatacc aggcaattaa tgtttcattt aagttgaaaa tcggggaggg aatatttaga
     3001 aatttgttaa ttttttataa aaatagctca gtttagctgt gtttatcggt tgagcaataa
     3061 attcatggtc ataaaaatat ttatagaaaa taattataaa aatatttagt caagatattt
     3121 atgtatggga ggatttattt tatgaaaaca cgtaatactt caaaatatga tgtcgatggg
     3181 ataccaccac taaaggaatc aatacctttg gccttacaac atctgttagc aatgatagta
     3241 ggaaatatgg tacctgcgat attaatagca aacgtggtag gacttaacca ggggcaagca
     3301 acaatgttga ttcagggttc tatgttagca gcaggtttag caacgttttt acagttatat
     3361 ccaatacctt tatttaaagg atttaagtta ggttcaagat tacctgtaat gatgggaatg
     3421 agttatgtat ttttaggagc ttgtctttca gtagcagcta aagatggttt agcagctcta
     3481 tttggagcac agattgctgc tggggtaatc gtattctttg taggatttgg agttaagaaa
     3541 attagacata tattcactcc aatagtttct ggaactataa tagcatgtat gggacttgga
     3601 ctattcgcaa cagctataaa gaatttagct ggaggagaag gcacacaaac atttggaagc
     3661 ccaataaact tcacagtagg agttatagtt gcttttgtaa taataatgat aaataaatac
     3721 ggtaaaggtt tagtaaaaaa ttcttcaata ttaataggaa tattagtagg ttatgctata
     3781 tctttagttt taggattggt tgatttctca gccgttcaag gagcagctat agtatcttta
     3841 ccaacaccag cagcttttgg attagaattt agaccagagt taatagtaat gtttactatt
     3901 atttacataa taggtatagc tgatatgatg ggtgcctgta caatagctac agtaggagca
     3961 atggatagag aggtaactga cgaagaatta gcatcagttg ttttaggaaa ttctataact
     4021 tctataattt catcactatt tgcagctctt ccaactggag tatttagcca aaatacagta
     4081 attgtatcta tgaacaaagt tacaagtaga tttgtaatag cactaggggc acttgtatta
     4141 ttactagcag ggatatctcc agcattgggt gcaataatga caactatacc atcttgtgtt
     4201 gttggtggag caactttagt agttttctca tcaatagcaa tgtctggttt ctctattatg
     4261 agtatggatg gattcactga agaaaacaat cttatagcag gtgtatctat agcaacaagt
     4321 atgggtctta caactgctcc acaagtttta gaccaattcc cagaaacaat tggaactgtc
     4381 cttggagggt caagtatagt ttctggagca ataatagcta tattacttca aacattattt
     4441 aaattaaagt cacgtaaaag tgctgaaaac gttacttcaa atcttgagga aaatattgga
     4501 taaatgtatt agatataata tgagaaaacc atatggtaaa atgttgtaat gaaagtattc
     4561 aaagggggat ttaagatgat tgatatacta ataaaaaatg caataattgt tacagttaat
     4621 aaagacagag atgttatatt tgatggagca atagctattc aaggtgacag aatcttggat
     4681 ataggaaata cagttgatat tgagcctaag tatcttgatg ctaaaaaagt tatagatgct
     4741 aatggaaaag taatattccc tggctttata aatactcata atcacctgtt ccaagtgtta
     4801 ttaaaaggtt taggtgatga tatggcactt catgagtggt taaatacaat gatgtttcca
     4861 tcagcaaaat tcttaacaga acaagatact tatgatgctg caatgcttgg atgtatggaa
     4921 ggtttaaaaa gtggtataac tacaatggtt gattatatgc atactcacaa tagaccagga
     4981 cttacagatg gaatagtaaa ggcatataaa gatttaggta taagaggggt tgtaggtaga
     5041 ggttgtatag atttaggtat tcacaaagag cttatagaag atgtggaaac ggttgaaaaa
     5101 gatttgagaa gggtattcga aaaatatcat aattcagaaa atggaagaat aaaaatctgt
     5161 gtagctcctt cttcaatgtg ggctatatct gaagaaatgg gtaagatgct ttggaatata
     5221 gttaaagaat atgattcata ttttacagtt catatatctg aaacagaatt tgcaagaaca
     5281 gcaacaaagg atatacatgg agaaatagat ataaaattac ttgaaaaatg gggtatagta
     5341 ggtccagaag ttgtagcagt acattgtgta tgtataactg atgaagacat ggaaatgctt
     5401 aaaaagtatg acataaaagt ttctcataat gtagcaagta atatgtatct tgcttctggt
     5461 gttgcacctg tgccagaaat gttaaaaaaa ggtataaatg tgagtttagg tcttgacggt
     5521 gcagcaagta ataacgcaca agatatggta gagcttatga aattaacagc tttacaacac
     5581 aaagtaaata attgtgaccc tctggcaatt tctgcagaga aagttcttga aatggctaca
     5641 attgagggag caagaactct tagaatggaa gatgaaatag gttctttgga gattgggaaa
     5701 aaagctgacc tagtaatatt taactcaatg ctttctccaa aatcaatacc tcttcacaat
     5761 cctgtatcaa ctttggtata ctcagctagt atgcacaatg tagagagtgt tttagttgat
     5821 ggaaatgtaa tacttgaaaa tggaaaagta actacaattg aaagtgaaga acaagtgttg
     5881 cttaatgcac aacttgcagc agaagaatta tgtaagagag ctaatgtaac taatagaatg
     5941 gaaggacata agtggaatag cttatactaa tattaaaata agattgtaat aatattctat
     6001 tacaacatta gtttatctgt aggaggagaa aatggaaaaa ataaaattaa atattaatga
     6061 ttacatgtat gaaatagaag ttgaaaagaa tgaaactctt ttgcatgtac ttcgtgaaaa
     6121 attaaatcta actggagcga aacaaggttg taatagtggc agttgtggag catgtaaagt
     6181 tttgatagat ggagttgaca tgaaatcttg taaacttctt gcaaaaaact gcgaaggtaa
     6241 agttatatac actatagaaa gttttgctaa tggagaaaat cttcatccaa tacaagaagc
     6301 ttttatagaa gttggagctg tacaatgtgg atattgtact cctggtatga ttataacaac
     6361 acaagctctt ttaaatagaa atgcatctcc taatgaagat gaaattaaag aagcttttaa
     6421 agaaaatctc tgtagatgca caggatatgt aaagattata gaagctgtaa aattatctgc
     6481 agctagatta gggggtagat aattatggaa aaggtaatag gaaaatcata ccctctaaaa
     6541 gatgcagctt taaaagttac tggtcaaatg aaatacgtgg ctgatatgaa accacaaaat
     6601 gtactttacg ctaagatgct tttaagtcct gtagcacatg caaaaataaa aagtatagat
     6661 acaagtgaag ctgagaaagt agtaggtgtt agagctatag ctacttatct caatacatca
     6721 caaagaaaat acaatggttc attaagattt tatgagcaca atatacctga aaatgaagtt
     6781 atattttcag atacagttag atttgttggt gaccgtgttg cagcagtagc tgcagagact
     6841 ccagaaatag cagagaaagc tgtaaaactt ataaaagttg aatatgaaga attgccagct
     6901 gtatttacta tacaagatgc aataaaagaa gatgctatct gcatacatgg agattcaaat
     6961 atagtttctc aaaacaacat aaatggggga gatgtagaaa aaggcttcaa ggaatgtgac
     7021 tatatatttg aagatgaata ctctactcca gcaatacatc atagtgcaat agagactcat
     7081 agtgtaattg cacactatga ttataatgat aaattaactg tacatacacc ttgccaaaat
     7141 acatttggat ttagaataat actatctcaa atttttggga tgtcattaaa taaagtaaga
     7201 gttataagac ctgctattgg aggctcattt ggtggaaaat tagaacttac aattgaacca
     7261 gtagcagccg cattatcaaa aatgacaaga agaccagtta aaatggttct tacaagaaag
     7321 gaatctatga tatcaacaag aactcgtcat gggtcttttg taaaattaaa aactggtgta
     7381 aataaagatg gtgaagtaat agcacaagat ataaaaatat atacaaatac aggagcatat
     7441 gcatcaagtg ctttaaatgt tataggagca cttagtcata aagtatttaa agtttataaa
     7501 ataccaaata taaagtttac tgggatgcct gtatatacaa atacaccaat agcaggagct
     7561 atgagaggct atgggtcacc tcaaatattt atggctcaac aggctcaatt tgcgaaaata
     7621 gccaaggaaa taggtatgga tttagtcgat tttcaaaata aaaatgcagt agagccagat
     7681 gatgtcgata taatcttccg cggttctctt ggaaatccta gagtattgga ttgtatagag
     7741 caaggtaaga agatgtttaa gtgggatgaa aagaaaaaac ttccaaaaga agaaggaaaa
     7801 tatttaagag gtataggtat ggcaataggt gcacatggta atggagtttt tggtgcacat
     7861 agagatgttg ttacattgac tcttaaatta aatgaagatg gtacattgac actactaaca
     7921 ggagcacatg atatgggtaa tggtgtcgta actatgcaaa caatgatggt agctgaagtt
     7981 ttaggaataa cacctgataa agttgataca tttgaaactg atacggatgc atgtacattc
     8041 aaccttggtg actatgcaag tagaggggta tttgtagaag gtggaggagc taaaaagaca
     8101 gcagagaaat tgaaaaacct tatagtagaa gaagcagaaa aattacttga aacatcaaaa
     8161 gaagatttat atctagacaa tggatatgta attagtaaac ttgatgaaaa tgtaaaagca
     8221 tcactttctg atgtcgcagt atatagtcaa agtaaatcat taagagagtt aactgttgtt
     8281 gaagattatt cttcaccagc tggacttact tcttatggtg tacattttgc tgaagtttta
     8341 gtagataaag aaactaagga tataaaagta gttgattttg tagctgtaca tgatgttggt
     8401 agagttataa atcctttaaa tttagaaggt caattagaag gtgggattca tatgggtcta
     8461 ggatacgcat tgagtgaaga attggcattt gatgaacaag gtagacctaa agctactgat
     8521 tttaaaacat ataaattatt tagaactgta gatatgccaa aatgtcaagt tgcatttgta
     8581 aatagttatg aaaaagctgg accttttggt ggtaagagta ttggagaatg tgctgttgta
     8641 ccagttgcgc cagctgttgc aaatgcagta tataatgcaa ctgatgtaga aatacattca
     8701 ttgccaataa aattaaaata gtagaaatta aagcacaaac aacaatttat tgtatttgta
     8761 aatagataat aaaaatagat ttatttggtg aggagatatt attatggcaa agatattagg
     8821 tatttgtgga agtccacgta aaggagcaac agaatatgca gtgttagaag ctttgaaaga
     8881 agcagaaact atacctggta tagaaactga gtattggtca gttagaggga aaaaaataag
     8941 tccttgtgta cattgtgatg catgtataag aaaagaaact atgtgtatta taaaagatga
     9001 tatccaagaa ttagagcaaa aaatattgga ggcagatgga attatagtag gttctccagt
     9061 ttatgatatg aatataactg ctcaacttac agcagtattt aatagacttc gtccaatgta
     9121 tttagttcat ccaggaaaac ttcaaaataa ggttggtgca gctataacta ctggtggaac
     9181 tagatatggt ggccaagaat tgacaaaact tcctataata aatttcttcc taatgcatga
     9241 aatgttagta tctggaggtt taggtggatg ctatataggt ggtacaatat ggagtaaaga
     9301 tggaaaagca aaaggtgccc aagaggatga gacaggtatg gacacagtaa aaagacttgg
     9361 aaaaggtgct gcagaggcag ttatggtttc taagtttggt cttgaaaaat ggaatgtcgt
     9421 aaaagaagaa ttagatgtta aaaaagatga aaaatcacct cttagagacc actaaagatt
     9481 tattatatta ttaaatatat acaaatagtg atgtaataga ttattgcatc actattttat
     9541 gttttaattt agattttata aattttatat taaaaaatat agaaacaaag tgttttgaga
     9601 tttattttta aattttaaaa atgagaatta aaaagtgaga agatagaaaa ggttttttag
     9661 ttattaaatt ctacatacat tgtatgaact aaagatagaa aagaaagaat aataaaagca
     9721 ttgcaatttc tatactaaag agtcatagaa aaatatctag tatatattta gtgttggcat
     9781 gttaattgct tatatattaa agtataaaaa tttaacaata atttggaggt attttagatg
     9841 ttaaaagaaa ttaaatggaa agtaaataat ttgccaaagg gtgataagga aaattgtatc
     9901 aaatttttaa atgaagaaga aataactaag gttagaaatt ttcacaaaag ctttcctcaa
     9961 tataaagaaa ctccattagc aaacctagaa ggtttagcaa aaaaattagg tgttgcagga
    10021 gtatatgtaa aagatgaatc ttatagattt ggattaaatg catttaaagt attaggtggt
    10081 tcttattcta tgggtagata cttagctcaa agattagata cagatatatc tgagttaggg
    10141 tatgacaaat taacttcgaa agaaataaaa gaaaaattag gagaaataac tttctttaca
    10201 gctacagatg gaaatcacgg tagaggagtt gcttggacag ctaataaatt aggtcaaaaa
    10261 tctgttgtat taatgccaaa aggttcttct gagtttagat taaataaaat taaaggagaa
    10321 ggagcagatg ctagtataac agacttaaac tatgatgatg cagtaagatt agctaatgat
    10381 tatgcagaag ctgatgatca tggagtaatg gttcaagata cagcatggga tggatatgaa
    10441 gaaattccag catggataat gcaaggatat ggaactatgg ctcaagaagc gatagagcaa
    10501 ttaaaagaat atggagtaga tagaccaaca catgtatttg tgcaagcagg ggttggttca
    10561 cttgctggag cagttcaagg atatgttgct tcaatatacg atgaatgccc aataacagta
    10621 gtagtagaag ctgatgaagc tgattgctac tataaatcag cagaagcagg agatggaaaa
    10681 ccaagatttg ttggaggaga tatgccaact ataatggctg gtcttgcttg tggagaacca
    10741 aatactatag gatttgaagt attaaaaaat catgctgcag catttgtttc agcaccagac
    10801 tgggtttctg ctaaaggtat gagaacatta ggtaatcctt taaatggtga tgaaaaggta
    10861 atttctggag aatctggagc agttacaact ggtcttttag tagcagctat ggaaagagaa
    10921 gatttagcag atttaagaaa agatttaaaa ttagatgaaa attcaagaat acttttaata
    10981 agcacagaag gagatactga ccctgacaaa tatagaagta tagtttggga tggagaatat
    11041 ccaagtatat aaggaattac taaaatgcaa ttattagata ttggtttaca tatttagaag
    11101 atatataagt tctaaaattt agaaaatata taagtatata aaatacatac attataaaaa
    11161 atctaggggg ataatgaaat gttaaacgaa caaagaaaac aagaagtaac tgaaatatgc
    11221 caaaaattaa ttcaaaatcc tagttcttct ggaaatgaag aaggtgttgt aaaagcaata
    11281 gaagagtctt ttgaaaaatt aggttttgat agttggtcaa gagatagata tggaaatata
    11341 gtaggatgta taaaaggtaa caagccaggt aagaaaatat tatttgatgg acatatagat
    11401 acagttccag ttccagatgc aagtaaatgg agtgttcctc cttttgaagg aaaaatagtt
    11461 gatggaaaga tatatggtag aggaacttct gacatgaaag gtcaatatac tgcaatgatg
    11521 tctgctgtag cgtattttgc taaagacact aatcgagatt ttgctggaga actatatgta
    11581 gcaggtgttg ttcatgaaga aatatttgaa ggtgtttcgg ctagagagat aagtaaggct
    11641 gttcaacctg actatgttgt aataggtgag tcttcagagt taaacttaaa gattggtcag
    11701 agaggtagag gagaaatagt agtagaaact tttggtaaac cagctcactc tgctaatcca
    11761 gaaaaaggtg taaatgcagt ttataaaatg gctaatgtaa tccaaaagat acaacaatta
    11821 gtacctccaa ctcatccagt tttaggtgat ggaatattag ttttaacaga tataaaatct
    11881 tctccatatc cagg
//
LOCUS       DACTGW010000113        11887 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.113, whole genome shotgun sequence.
ACCESSION   DACTGW010000113 DACTGW010000000
VERSION     DACTGW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11887
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.113"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            177..1151
                     /locus_tag="I9573_002307"
     CDS             177..1151
                     /locus_tag="I9573_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796923.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYKLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNHPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(1267..2298)
                     /locus_tag="I9573_002308"
     CDS             complement(1267..2298)
                     /locus_tag="I9573_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAT4796924.1"
                     /translation="MKKIRKLATKLLITTIVLVSGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNNKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPTVGAVAQTQKPALPGKHTMLPHQDGAK
                     VVGKFGDIKDKHVMYEGVRCVVINSSVNTVTFLGSKSNLDPSNLTIAVIGFMNTPYGM
                     FALAPEGEPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIEITDEKANIGL
                     GQIMYIRYE"
     gene            complement(2910..3761)
                     /locus_tag="I9573_002309"
     CDS             complement(2910..3761)
                     /locus_tag="I9573_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT4796925.1"
                     /translation="MSCILKLKQIFEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(3778..4698)
                     /locus_tag="I9573_002310"
     CDS             complement(3778..4698)
                     /locus_tag="I9573_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HAT4796926.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDGIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(4808..5722)
                     /gene="murQ"
                     /locus_tag="I9573_002311"
     CDS             complement(4808..5722)
                     /gene="murQ"
                     /locus_tag="I9573_002311"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAT4796927.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNRVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(5821..6918)
                     /locus_tag="I9573_002312"
     CDS             complement(5821..6918)
                     /locus_tag="I9573_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAT4796928.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEKALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFDFKPSTK"
     gene            complement(7151..8497)
                     /locus_tag="I9573_002313"
     CDS             complement(7151..8497)
                     /locus_tag="I9573_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT4796929.1"
                     /translation="MLDKFTEFLDSKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(8586..8894)
                     /locus_tag="I9573_002314"
     CDS             complement(8586..8894)
                     /locus_tag="I9573_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4796930.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEASVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(9155..9544)
                     /locus_tag="I9573_002315"
     CDS             complement(9155..9544)
                     /locus_tag="I9573_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796931.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSNKNTKKEMEKNQRTDYNIPTISFK
                     SGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRGFSL
                     GFFRLYILFVLSFAIIGYFWIYKGIHI"
     gene            complement(9546..10706)
                     /locus_tag="I9573_002316"
     CDS             complement(9546..10706)
                     /locus_tag="I9573_002316"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAT4796932.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAIIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDKNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGVHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(11136..>11887)
                     /locus_tag="I9573_002317"
     CDS             complement(11136..>11887)
                     /locus_tag="I9573_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4796933.1"
                     /translation="ENLIKDIIENLKIKNNSDIETYKQSLKELDDYIAKWVHEIKIPI
                     SSLSIITDRLSSIEDSLDIKNQVAKINFLVNSILYSSRSNTMFEDVFINKFNLEKLVK
                     MSIKNNSFLLIKNNVEVSLNELENDVYTDSKCMSYVLDQIINNAIKYSKEVGKIEFNS
                     KKLENGVVLSIKDFGIGINEEDISRVFDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSL
                     GHEIEVCSKIGSYTIFNIYFYDISDYLSLDS"
BASE COUNT         3799 a         2085 c         1320 g         4683 t
ORIGIN      
        1 ggtctcatgt tagtctctaa atttaaaatt ttacaaaaaa ttttcataaa ataaaattat
       61 attgattgta tctaaacaaa tagggcaata aaataattaa gtatagttac ttacctttca
      121 agcaatatta gttttatgtt agaattttat tatattgatt tacaggaggt ttgcatttga
      181 ataatattaa ttcacttaaa gtaataaaat tatctcttgg atgttctctt gctatattca
      241 ttgcttggct attaaaatta gaatattcaa tggttgctgg agttatagta ctacttacag
      301 taaaggacac taaaagagaa actcttaaag gctccattgg aaagatttat ggttttcttt
      361 tatgtactat tttttcatat ttatgtttta atattttagg atataatttg acttcatttt
      421 caatatttat atttataatt ataccacttt gttttttgtt aaatattcaa gatgttattg
      481 ctatgtgtgt agtcatagca tcccattact accttcaagg agaaacttct atcaaatgga
      541 ttttaaatga agctggaata tttgcagtag gaactggaat aggtgtctta ataaatatgt
      601 atattccaac aaatatacat aaaatccatg aagttcaaaa aaagttacag gaagaagtga
      661 gtattgtact tatagacatt gcagatatta ttgtaaatcc taaaaaagaa aatggatata
      721 gtagagattt atataaatta aattcactta ttgacagcag tatttctgaa acttacgata
      781 atattaataa tactttatta agtgatacta gattcttctt agaacatatg gatattataa
      841 aaagccaacg ggatatctta gaaaatttgt atagttatgt atctcaacta aattctacac
      901 caccacaagc tcacattcta tctgctttca tacataaaat aggttatact gaatttgaag
      961 ctgaaacagg caatcttctt cttgaagaat tgaagagatt aatgcttagt atgaaaaacc
     1021 atcctctacc tgttgacaga acagagtttg aaaacagagc tattttattc ctatgcttga
     1081 ctgagttaaa acaattttta gttaatcgta aacatgctca gatgcttaga gataataatt
     1141 tttataaata aaaaggtgag aaatccagca gttgacctga ctacaaaata tcagatataa
     1201 tattaaatgc gcccctagta agagggcgcg atgtttataa cttattctac tgtcatgatt
     1261 aaatcattat tcatatcgaa tatacattat ttgaccaagt ccaatattag ctttttcatc
     1321 agtgatttct attttaatag tcacatcatt ttttttacag tactttgcta attctgcacc
     1381 agaacgattt ttaccactaa aatctaaaga aaatggctta tcatatttaa aaggatattc
     1441 aacaggttca ccttctggtg ctaatgcaaa catcccatat ggtgtgttca taaatcctat
     1501 tacagcaatt gtaagatttg atgggtctaa atttgattta ctacctagaa atgtaactgt
     1561 attcactgat gaattaatta caacacatct aacaccttca tacattacat gtttatcctt
     1621 tatatctcca aattttccaa caacctttgc cccgtcttga tgtggtaaca tagtgtgctt
     1681 cccaggtagt gctggttttt gcgtttgtgc tactgctcca actgttggat atgctgtctt
     1741 atcaaatggt aatgggtggc taagactctt gtatatgata tcctttcctg ctgatttatt
     1801 agcatcaata tctacatgat acaaacgccc atcaccaaaa cgtataagat tccactggtg
     1861 gtcatttgat gtgctgtgta cataataaga ttcaattccc atagcgtcac acaaagcttt
     1921 catgctccta gccattcctg aacatgcacc ttttttagta accaatgtat aggcagtatt
     1981 cgctccttct actgcattcc agtcaacata tgttgtgtta tttttaatgt gattataggc
     2041 aactaaaatc ttagcgtctg tagtcatgtt ctttgtaatt ttattgttca taaaatctgc
     2101 tgttgcttgt gctactgagt ttagttgggc ttgtgacagc tttggaccat atacgctatt
     2161 ggttccagtg acatagcttt ttggtgtttt tgctgttacc tctttggctg tcataccata
     2221 aactgtcata ctcatacctg agactaaaac aattgttgta atcaataact tggtagctaa
     2281 cttcctaatt ttcttcatac ttagtattct cctttataaa atattgttac ttcctattct
     2341 ctaccattaa taaagttcat ttgggattta aaaaggtgga ttgtcaatat gtatgtttct
     2401 tcatttatac gttcgtgaaa tatttaaatt tcatggaaaa tctaatagat attttctaga
     2461 taatttgtat acaatacaac tcgcaaatca gggtgtttgc tgttagtttg agaaaatgat
     2521 gatgattaat atattcggaa tctttgacct ataataaata ccattgtctt caatcattaa
     2581 taatggtaag agtaaagaga attatcaata atacagttaa aatcaacttt gtatacattt
     2641 catacattct ctcccaacaa cattatactt cttataattt gtatattcaa tacgttttct
     2701 gggaaaatat aggatatatc tataaaaata taaaaaagga gttgaactta aaatttgatt
     2761 aaaaaaaatc aattttaaga caactcattt gtattataaa aatgctatat attattatta
     2821 agtaagtcaa tattttagat atttatctat ttcttattga cttacgctta tagtctaact
     2881 tatttgttta gttgatatat aacctagttt taagcattat taatatttaa ataatcaagt
     2941 atcctttttg tttcttgtag tttctgttct gcctcattta agttttcttg aattatagaa
     3001 atatatatta tatctatcaa cgtaagcatt gctattcttg aagaaatagc tccttctcta
     3061 agcctttttt ctatatttgg tactactagc tttaaatcac taatcttact aagatgattg
     3121 tcactatatt tagttataca aatgacctta catcctttta attttgcatt ttctatagac
     3181 tttattactt cttttgattc tccagagtaa gatattgcta tagctatatc ttctttatct
     3241 acatttgaag ataccataag ttgtgtatga ctatctaaag aggtaaatgc ctgtttattt
     3301 atccttaaaa gtttcatctg taaatctagt gcaactaaag ccgaaccacc tactccaaat
     3361 atatatacat ttttagcctt tattatctgt tcaactgctt cttttattat atctttttca
     3421 ttcaacaacc ttgtttggtt gatagtctct atgtttttgt ttgtaatttt gtttataaca
     3481 ctatctatat tacaatcata gttttcatct acatatgtgt attcattttc tttgcccatc
     3541 aaatctttag ctatctcaat ttttaaatcc ccaaaacccg aatatcccat ttttctagaa
     3601 aacctaacta tacttgctgt acttgttttt gagtttgacg ccaattctga tacagaaagc
     3661 ttacttatag cctctgtatt attaagtatg taatcagcta tttttttatc tacttccgtt
     3721 aaatcttcaa atatctgttt taattttaaa atgcaactca tactctacca cctctaccta
     3781 tttactataa tatttcttct tagcaagtaa acatgctcct aaatctggag tcaacactgg
     3841 tctcactata ccaaatctcg ttttattcaa atatttttta agtggctcta atattaaatc
     3901 tcctgactta aacactccac ctgaataaga tacctttatc tttgtacctt gttcaaaatg
     3961 atgctctaat cctattataa gcctacttaa ttcataagct gcatcatcaa atattttact
     4021 agcagtatta tcaccttcaa taactgcttt taaacatatc gtagcaaact ttgctatttc
     4081 tattctatct ccttgtattc catcatttac atattttatt atgtcataat cattctctat
     4141 attaagatgt ctttttatta aatcatataa ttttgtcttt gaacaccttc catcagactg
     4201 cattgtgaac atttttaagg tagccattcc tatataatac gctgaacctt catctcctaa
     4261 tacatagccc catcctccac atctgtgaag attcccatct ttatctaaag cctgtgctat
     4321 tgaaccagta cctgcaatta tatttatacc tttttcccca tttaaagaac ctgctaatgc
     4381 tatttctaca tcattaccta gagtataatt tatatgacta tatgcatttt ttgttgctac
     4441 ttctaatgca tacaatactt cttttatttt tccatatcca gcaagtccaa caaatgcata
     4501 cgttatttct tctacattta tcttagcatc tttacaaact tcttccaacc cagttattaa
     4561 taatttcgtt agattatcaa atcctatttg attataatga caagttcctt tggttatggt
     4621 tcctactata tttaattctt catctactaa tgtaaatgtt gttttcgttc ctccaccatc
     4681 tacacctaaa aaatacatac tatcctccta caatatcttt aataattctt tcaatactta
     4741 atatctaaaa ataaccttcc aatgctgttt ttttaatatt gaaaggttat cagtactatt
     4801 atttatttta caatttttct attgatttta aagcttcact tatataacct tcttgtctat
     4861 ctaggatact ctttgcacta tccttatcta aaccactttt tatcataaat atagccaatt
     4921 taactctatt atctgcttct tctaagaatt tttctgcaac gtcatatttt acatttgtag
     4981 ctatcattat tattcttttt gctctttcaa ccaacttttc atttgatgtc ttaacatcta
     5041 ccatcaagtt tgagtatact tttcccatct taaccataga agctgttgtt aacatattac
     5101 ataccatttt ttgagctgta ccacttttca ttctagttga acccattatt acttctgccc
     5161 ctactataat gtttattggt aaatccgctt ttttactcat ttcactattc ggattcatag
     5221 ttattgatag tactgcacac cccaattcct tagcatactc cattgcacct ataacataag
     5281 gagtccttcc tgatgcagct ataccacata taacatcctt agatgtaact cctctatcta
     5341 ttatatcttg ctttccaagt tctttatcat cttcagcacc ttctattgct ttaaatattg
     5401 cttgatttcc accagctatt atagcttgaa caagctcaaa atccactccg taagttggag
     5461 gacattctgt ggcatctagt attcctatcc tcccactggt acctgctcct atatagaata
     5521 atctacctcc ctgcttcatt ctatctacta ttatattaac agcttttgct atactctcct
     5581 tttgttcttc aacagcatag gctacagttt tatcttcgtt atttattaat tctattatgc
     5641 ctaatgtatc ttctttatct atttgcaatg tattcttatt tctaccttct gtaactaaat
     5701 tttctaaagt ttttattaac atatcatttc ctcattttct taatattaaa tttttaaaca
     5761 cattttaact ccaactttca taattatatt tttataataa attaatattt tcataataaa
     5821 ttattttgtt gagggtttaa aatcaaactt ttgccatggt ttaatataat ctaatataaa
     5881 taattcttct tttcttatat atcctactac attcgattta ccactgtttt tcatatcagt
     5941 caaggctatt tgtaactccc ctgcataatg accatattca ctactttcaa ttactatatc
     6001 tcctcttttt attattttag gagtattaaa tagttcaaat ttgtatcctt tatatttaac
     6061 acgagattga gtagacctta tcagactgtc agatatatcc cctctattaa aatgtatttc
     6121 ttcaaagagt atcttcttct caacttctcg tatattttcc tctattgcta catcaaatgt
     6181 aaccatatct tttctcatag aacctaatac ctttagctcc tcatatgttg gataacaatt
     6241 tgatattatt atatcattta tattattcat agctattata tgctttactt gaatatctat
     6301 aggtagattt ctgtgcatct caattgttgg aagcccatca gttacaggcc atggtccaaa
     6361 tgtggtttca ttttgacttg ttacaaaaac tgctgttctt aatccatact tattaaatct
     6421 ttctgtacat tttataaagt gttctaaatt taatccacta tatctatgtg gataaaagtt
     6481 atgacatcct gtcaaatttt ctttatttgg ttggtagtcc attattgtat ctatataatt
     6541 agtattatta ctcatattga tttctatttt aagattttga ggattaaaag tcattaaggc
     6601 cttttcagaa cctgtaaatc ccatatcaag ccttattccg tctgccttcg tctttttaaa
     6661 aaaagtcaaa tctttgtagc ttatttctaa ttcaccaaat actctgggat ttacatcaag
     6721 tattacctca aaacctttaa acttagcgta ttcatttata cttttaaatt ttttaattat
     6781 attttcttta gtatctttaa ctgataataa acatgaaaat atacgtgtaa atccattttc
     6841 agcagcttta tctatataat catatatttc cttagatgtt gttttttctg gataaattga
     6901 tattcctaat cttcccatct gtgtcatctc cttatatcct atatgtgttt aaatttattt
     6961 cgaattacac gaaacttttt taaaaatttg gtgaaaaaaa gtttcatata tatttataaa
     7021 aaaagaaaag tttacctttt ctttttttat aaacaatcaa caaaatatct tcgttactta
     7081 tttcataact gttatacttt gaaatttata ctaatctaat atagttttta atttccaata
     7141 tagtttctaa ttaataagct tcttgctctt caactttttc ttcatgaagc aatctcttat
     7201 cgtacatctt gacaaatgga tagtaaatta ttattgatat agctatacat aataagttaa
     7261 gaactgaagc tctccaatct ccaccagtgg ctaaatatgc ccctactggt cctggtaatg
     7321 tccaaggagc agtaactact actctggata ctaaattagc actcatagca aaccatgaaa
     7381 ctgttgccat tactattggt gccacaacaa atggtattat taatattggg tttaatacta
     7441 ttggtgctcc aaatattatt ggttcattta tattgaatat tgctggtatt acagttgctt
     7501 ttcctaaaga ctttccataa gctgattttg ctctaaatgc aagaagtaaa gctaagccta
     7561 ttgtagctcc agaacctcca atccatataa accattgaaa aaagggttca gcagcaatac
     7621 ctggaactgc ttgacccgca gcatatgaag ctgtattagc atctaataat tgtatccata
     7681 aaggtcttgc caatgaacct attattgata caccatgtat tccaaatgac caaaaaaacg
     7741 ttattaaaaa tattagtata attactgatg gtaatgtgtc agtggctgaa actaatggag
     7801 ttactaattt agctatgaag ccatgccaat caaaccctag gaaataagta acaatagcca
     7861 ttaaaattat tattaatgcg gctggtgtca gagcttcaaa agaccttgtg actgatgctg
     7921 gaactgcttc tggcatggtt atcttgaatt tagatttgtc agtaaatcta taaatctcaa
     7981 cacctattat agatgttaca atagcaacaa acattgaacc tccaccaaga ttagccattg
     8041 gcatttgcca tccttgagga attgaattat accaagctaa aagttctgga ctcttttcaa
     8101 ctatctttaa taaatcaggg gtaagaccct ctattgcagt aggaactatt gtcaaaagga
     8161 atgtagctgt agctaataaa cctcctgtca atccatctaa cttatatgac tttgccaaac
     8221 tataacctat gccaaatgta gaatatactg atataataaa aaatgtcatt ctatatggta
     8281 gtaatattgt aactgcattt tcctttagat actgagttac cccccatgaa tctggtaatg
     8341 gaggaaatgc taatatcaag aaaaatgacc caactattat aagtggtaat gaagctacga
     8401 ttccatctct tacagctctt aagtgacgtt gttctgccaa tctggccata gaagttgata
     8461 atttactgtc taaaaattct gtaaatttat ctagcatctt ttctctcctt aattaatatt
     8521 tatttttatt atcattaaaa tttttataaa attaactttc ttctaaccta acaacactgc
     8581 aactactatt tactcaacaa ctcttgaact tgttttaata actttggtcc tcctaatgga
     8641 ctataagctt gtgcttgtat taatgcacat ggtacactag cctcatcagc aaaagctttt
     8701 aaatagtcaa atctatgtct gacttgtgga gctaccatag caatatccca tccattttta
     8761 acttcattct caaactcttg agtaccaaca gccaaaacat ctatatctat tccatttttt
     8821 gctccttctt tttgtaatgc attaactgct atagcacttg acatccctga tgaacaaaca
     8881 aataatactc tcatattgat tccccctgtt tttttatttc ttttcaatca ttatagcatg
     8941 tatttttgat tacaggaata cgtttgtata tattttttca tattttacat ttttaattgt
     9001 aattttattt catgcattta tatcttgctt taattgcttt atttatctaa tcattctttt
     9061 gtctaaaatt acacactact taaaatactc atatcttttc ttaaaaaaga aaaaagacta
     9121 tcttttatta acataaaata gtctcaatac ttttttaaat atgtattcct ttatatatcc
     9181 agaagtaacc tattattgca aatgataaaa caaataaaat atacagtcta aaaaatccca
     9241 aagaaaaacc tctcttagtt tcaacaaagt atcttgtata tgcaactgca aaagatgtaa
     9301 ttaatgaatt tcctataaca actccaaatc tattatctat tccaaatata gataataaca
     9361 aaggaaaaac tacagtactt actagagatg aaactattag tattaatcca cttttaaaac
     9421 ttatagttgg aatattataa tctgttcttt gatttttctc catttctttc ttagtatttt
     9481 tattactttt tgcattttta taattattat ttttctttct tttctttttt ttattattag
     9541 acatgtcatt tacctccaaa aggtttaggt gttatattcc ctaaaataac tttattttta
     9601 gcccctgttg cagatattac attagaaaaa gaattattta atgtttcatt gcccaagatt
     9661 acaaatgcca aagcctcctt tgcatcagaa gaatacccat attcatcttg agttaatact
     9721 tcaacctcta ataataactc ttttatatat cctattaaag ttagattatg aactcctcca
     9781 cctccaatta ataaagtatc tacattaacc tttggaagta tgaagttttt ataattataa
     9841 tatatagctt ttgccgtaaa catagttact gttgctatca aatcatattt atctacatgt
     9901 ttatatttgt taagcatgtt atccaaatag acttgtccaa atacctctct tccagtgctt
     9961 ttaggtggcg cttgactaag atacttatga ctcatcaaat cttttaacat ttcttcatta
    10021 attttacctt ttgatgcaaa gtatccattt ttatcatact ttctattaaa taatctttga
    10081 catactccat ctattatcat atttcctggt cctgtatcaa atgcaaatac atcatctatg
    10141 ttacatgatt taggtattat agtaatattt cctattccac caatattttg aagtgctaaa
    10201 tttttatttt tatcactata taataaaaat tcactatatg gtactaatgg agcaccttct
    10261 ccacctgctg ctatatccat tactctaaaa ttagatatta cctttgtatt tgtttcatat
    10321 gctataattg ctggttcacc tatttgaagc gttgaagata caaaattatt ataacttctt
    10381 ggtatatgaa agattgtttg accatgagat gcgataaaat ccaaatttgc tatatgaaag
    10441 tttgctttat gacacactga ttttacagct ttactaaata aatatccaag cttaaaattt
    10501 aagctacaga ttaaatcaac actcgattcc tcttctatac agcactttct tatttcgtct
    10561 tttatatctt taggaatttc tagagttata aattctattt gcttaacttt agtatttcta
    10621 ccattcccac taatttcagc tagtactgca tcaactccat ctaaggaagt tcctgacatt
    10681 aaacctacac tatacatagt tatcatcctt ttaagtttat agttattcct ccccaaatca
    10741 aggtattaag tttaataatt ttagaattaa tcttatttca tctttaattg cgataattta
    10801 ctcttacgaa attaatcgag ttaatcttat acacgttttt taccttagct aaatcttaca
    10861 ttaatttatt ttcagtgtca atgtaatgca tgtaattttt tttcacaaac aaaacatatt
    10921 ctctctccaa aaaccatatt aaaatagtta ctacaaataa aaaacatacc aggtacctat
    10981 ctgatatgtt tttcatatat aataattatt attttataat atagatagaa catcatttaa
    11041 ggcactaatt aaatcttcac tattttcaag aactaaattt attcttattg tatatgtcga
    11101 tttgtatatg atgatagatt ttatagtcta ataacctaac tatctaggct taaataatcc
    11161 gatatatcat aaaagtagat attaaaaatt gtatatgaac caattttcga acatacctca
    11221 atctcatgcc ctaaactatc tatcattttc tttacaaaat acatccccat tccagtagac
    11281 ttatacaatt gatttcttcc atttttcccc gtaaatccct tgtcaaaaac cctagatata
    11341 tcttcctcat ttatacctat accaaaatct tttatagata ataccactcc attttctaac
    11401 ttttttgaat taaattcaat cttaccaact tcctttgaat actttatagc attattaata
    11461 atttggtcta aaacatatga catgcattta gaatctgtgt aaacatcatt ttctaattca
    11521 ttcaaactaa cttctacatt attttttata agcaaaaatg aattattttt tatagacatt
    11581 tttaccaatt tttctaaatt aaatttattt ataaatacat cttcaaacat agtgttgctt
    11641 ctactactat acaaaataga gtttactaaa aaatttatct ttgcaacctg attcttaata
    11701 tccaaactat cttctatgct acttaaccta tctgtaatta tacttaaaga agatattgga
    11761 atttttattt catgaaccca tttagctatg taatcatcta attcttttaa tgattgttta
    11821 taagtctcta tgtcactatt attttttatt tttaagtttt ctataatatc ttttattaga
    11881 ttttctt
//
LOCUS       DACTGW010000114        11879 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.114, whole genome shotgun sequence.
ACCESSION   DACTGW010000114 DACTGW010000000
VERSION     DACTGW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.114"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            144..2384
                     /locus_tag="I9573_002318"
     CDS             144..2384
                     /locus_tag="I9573_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAT4796934.1"
                     /translation="MSNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNDIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSRDGEISEATKKKLSKLLIEDRCPICDGRRLNEGVYRSLI
                     NGYNIADLTSMQIDELAEVIKSINEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHVVDVGPKAGKYGGKVVYEGSYKNLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEIKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQRKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            2798..4333
                     /locus_tag="I9573_002319"
     CDS             2798..4333
                     /locus_tag="I9573_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAT4796935.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTAMTISGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGIGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNISDLKNVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVIVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSEVFKMVGDILTDKEMAKMNYDVENNNLEPREVACEFLNKK
                     GLL"
     gene            4349..5110
                     /locus_tag="I9573_002320"
     CDS             4349..5110
                     /locus_tag="I9573_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008751341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796936.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            5448..6316
                     /locus_tag="I9573_002321"
                     /pseudo
     CDS             5448..6316
                     /locus_tag="I9573_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            6510..6761
                     /locus_tag="I9573_002322"
     CDS             6510..6761
                     /locus_tag="I9573_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAT4796937.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            6873..7652
                     /locus_tag="I9573_002323"
     CDS             6873..7652
                     /locus_tag="I9573_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT4796938.1"
                     /translation="MNNTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(7787..8437)
                     /locus_tag="I9573_002324"
     CDS             complement(7787..8437)
                     /locus_tag="I9573_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAT4796939.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIDHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(8434..8937)
                     /locus_tag="I9573_002325"
     CDS             complement(8434..8937)
                     /locus_tag="I9573_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT4796940.1"
                     /translation="MKLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGRFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(8986..9501)
                     /locus_tag="I9573_002326"
     CDS             complement(8986..9501)
                     /locus_tag="I9573_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAT4796941.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCIDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            9817..10308
                     /locus_tag="I9573_002327"
     CDS             9817..10308
                     /locus_tag="I9573_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT4796942.1"
                     /translation="MRTIDIKGENMRELLKWVSIINRYNTNYIDRKLADIGINNSQYF
                     YVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTE
                     KSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENG
                     RKI"
     gene            10292..11695
                     /locus_tag="I9573_002328"
     CDS             10292..11695
                     /locus_tag="I9573_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT4796943.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
BASE COUNT         4454 a         1324 c         2054 g         4047 t
ORIGIN      
        1 taaaatctac atgctataat ttgacctgtg tttataaaaa aaggagtaat ctaataaaat
       61 agtatttcac ttatgtataa ctaacaacta ttattacgat tttctctaaa taatttaaag
      121 aaaaataatg caggaggtaa aatatgagca atgaatttat aattttaaaa ggttgcaaag
      181 agaataattt gaacaatatt tccttaaaaa taccaaaaag aaaaataacc atattcacag
      241 gggtgtcggg ttctggaaag tcatctattg tatttgagac aattgcaaaa gaatcacaac
      301 gccaactaaa tgaaaggttt agtacatttg taagaagttt tttacctaag tatggagagg
      361 ttaaagctga ttgtatagaa aatctgtcaa caccaattat catagaccaa agtagacttg
      421 gtggaaattc aaggtcaacc ctaggtacta ttacagatgt aaattccttt ttaagagctt
      481 tatattcaag gtttggtagt gagtatatag gtaatgctaa tatgttttct tttaatgata
      541 ttgatggtat gtgcccacac tgtcatgggc ttggaaaaaa gctagttcct aatatgaacc
      601 aaattcttga tatgaataaa tcattaaatg aaggtgctat attgctttca gggtttggag
      661 tgggttcttg gcattggaat atattcgctg aatcaggatt ttttgatatt aataagagga
      721 tatgtgatta ttcagaagta gaattggaaa agtttttgca tggtaaagca gaaaagatta
      781 agatagaaaa tgctggtcaa actaacatga catatgaggg attaatggtt aaatttaatc
      841 gtctatactt aagtagagat ggagaaatat ctgaggctac taagaaaaaa ttgagtaaac
      901 ttcttataga agatagatgt cctatatgtg atggaagaag acttaatgaa ggagtatata
      961 ggtctttaat taatggttat aatatagcag atttaactag tatgcaaatt gatgaattgg
     1021 ctgaagttat taaaagtata aatgagccag aagcacaacc tttaattaaa ggaataatag
     1081 aaaagttaaa cagtatcata gatataggtc ttggatattt aacactggat agagagacat
     1141 cttctctatc aggaggggaa tctcagcgta taaagatggt taagtattta aatagtaatc
     1201 ttgttgattt aatgtatatt tttgatgaac caagtgtagg acttcatcca aaagatgtgt
     1261 ataaattaaa taatctttta aaaaaattgc gtgataaagg gaatacaatt atagttgttg
     1321 aacatgaccc agatgttatt aagattgcag accatgttgt agatgtagga ccaaaggcag
     1381 gtaagtatgg tggaaaagtt gtgtatgaag gcagttataa aaatctactt acaagtggta
     1441 ctttgacagg gaatgcttta agtaaatttc ttcctattaa agaaagtgta agagaacata
     1501 gtggatattt agaaattaaa aattgtaata aaaacaattt aaaaaatgta tctattaaga
     1561 tacctaaagg tgtattgact ctagttactg gagtagcagg agcaggtaaa agtacgttga
     1621 ttaaggatga atttttaaag caaaatccta gtgctgtact tatagaccaa agccctgttt
     1681 cagcaaattc aaggtcgagt ttggcaacat atagtggaat tatgaacaac ataagaagca
     1741 tattttcaaa aacaaatggc gtaaatgctt ctttattcag ttcaaattca gaaggagctt
     1801 gtgataactg taagggttct ggaatagtag aaatgaatct tgcttttatg gagagtataa
     1861 agagtacctg caatgtttgt gaaggtaaaa aatttaaaaa agaagtttta gaatataggt
     1921 ttcaaaggaa gaatataatt gaagttcttg aaatgtctgt tttagacgca attgagtttt
     1981 ttaatttaaa acagattaaa acgaaattac aatgtattga aaaaatgggt attggatacc
     2041 ttactttagg acaaactctt gatactttgt ctggaggaga gtgtcaaaga ttaaagcttg
     2101 ctagtgaatt aaataaggaa agttcagtgt atatattaga tgaaccaaca acaggtcttc
     2161 atatggctga tattgagaat tttataagta taattgaaga gattgtagat aatggaaata
     2221 cggtcataat aattgaacac aatatagatg taattaaaag agctgattgg attattgagc
     2281 tgggacctga aggagggaca aaaggcggga gagtaatatt tgaaggtatc ccaaaacaat
     2341 tatgtaacaa taaaatatca ttaacagcaa aatatatagt ataataaata aaatataata
     2401 aaattataat gatattttaa taaatataat gggtatataa aatgataatt aaacttaaca
     2461 gtaataaata atgtgagaaa tattcaaatt taattaaata aagtgtcaga aatttgttta
     2521 aattttagta ttttaacgat tctgaccttt tttcttattt ttatggagag tatcgaagat
     2581 ggagtctcta atagttactt ttttgtgtct acttgcaagt ttgattaaaa aatggctata
     2641 cacacttgtg acttcagtca tgggaggttc agattataat cttaatatat aatttgtgaa
     2701 ataatttgat gacgatttgc tgatgtataa acaataaaga ttaactacat taaaatttaa
     2761 atcttgttaa gtaaaactta taaaaggaga gagatggatg ataaatgata ttctaacctt
     2821 attatctgaa agaagtgatt tttttataaa gctatccata gaacatttga tgatttctat
     2881 tatttctata ataattgcga ttgttttggg tggtataatt ggtattattg taagtgaatt
     2941 tcataattct tctaaaccta tattgggaac tatcaatttt ctatacacca ttccatctat
     3001 ttctatgcta ggatttctga taccattttc cggaattgga aatactactg ctgttattgc
     3061 acttaccatt tatgcattat tgccaatggt tagaaataca tatactggaa ttactaatgt
     3121 ggataaaaat attcttgaag cagctagggg aatgggcagt acagattttc agatattgta
     3181 taaaataaaa cttccattag ccctacctgt tattatgtct ggtattagaa atatggtgat
     3241 tatgacaatt gcattagctg gtatcgcttc atttataggt gctggaggcc taggtgtagc
     3301 catatataga ggaatcacga caaataacac agcaatgaca attagtggaa gtatacttat
     3361 tgctctatta gctttaatat ttgattttat tttagggctg gtagaaaaat tttttcaacg
     3421 aaaaagaaaa aagaaaaata ggaaaaaagt ttttgccatt ctaggaagcc ttattcttat
     3481 agcaactatc tttacgtctt atttatttaa tccttcaaag actcaaacta ttcatattgc
     3541 tactaaacct atgactgaac aatatattct tggtgagatg ttggatattt taattgaaaa
     3601 aaacacaggt ctaaacgtgg aaattacaca aggcatagga gggggaacat ctaatatcca
     3661 gccagctatg gagaatggag aatttgacat ttatccagag tatacaggta caggctggaa
     3721 tatggtactt aagaaaaatg aattatatac tgaaagtatg tttagtaaac tacagtcgga
     3781 atacaaaact aaacttaaaa tgcaatggac tggtatgtat ggtttttcta atagctatgc
     3841 attagcagtt agaaaagaag ttgcagataa atataattta aaaaatatat cagacttaaa
     3901 aaatgtatct tctaaattga catttggagc tgaatatgac ttttatgaga gagaggatgg
     3961 atatactcca ttgtgtaata catatggact gaactttaaa gatacagttg atttagatat
     4021 tggattaaaa taccaagcta tcaatgaagg taagattgac gtaatgccta tatttacaac
     4081 tgatggtcag tatagtgttt cagatgttat agttctaaaa gatgataaaa atttctaccc
     4141 atcttatcaa tgtggaaatg ttgttcgttt ggaagtatta gaggaaaatc cagaactatc
     4201 agaggtattt aagatggttg gagatatttt gactgataaa gaaatggcta aaatgaatta
     4261 tgacgtagaa aacaacaatt tagaacctag agaagttgct tgtgagtttc tgaataagaa
     4321 aggtctttta taaattggag gaaatataat gacacctatt atacaattta aaaatattaa
     4381 aaaacaatat aatgataaaa ctataataga taatcttaat ttagatatag aaaaaggaga
     4441 gtttttgact gtaattggtt cgtctggttc tggtaagaca acactcttaa aaatgattaa
     4501 tggtctgatt ttgccagatg gaggaaatat tttgataaac caaacagata tcaaaaacga
     4561 ggacttaatt aaattaagaa gaagaatagg atattgtgta caaggaagtg ttctttttcc
     4621 tcatatgact gttgaagaaa atatttcata tgtaccaaat ctcctaagta aaaagaataa
     4681 attagaagta aaaagtgctg taaacaagtg gatggaaata gtgggattgc caaatgatat
     4741 gaaagtgcgt tacccatcag aattgtcagg tggtcagcaa caacgtgtag ggattgcaag
     4801 agctttagct tcttctccag aaattttatt aatggatgaa ccttttggtg cagttgatga
     4861 gattacacga aagcaattac aaaaagagat aaaagaaatt cataaaaaaa caggagttac
     4921 gattatcttt attacgcatg atatttatga agctttgatt ttgggaacaa agactttggt
     4981 attaaatcat ggagttgttc aacagtatga tactcctgag aatattttaa atactcctgc
     5041 taaccaattt gtagaccagt tattgaatat aagaaaatct ataatagatg atgataattt
     5101 aaaagaataa atttaaattt aaaatcaaat tgttgtgata aaaactattg atttagagct
     5161 aacttcaagt tgtatacttg aagtggaaaa gttagattaa ggtatagaag agaatttagc
     5221 agttggctgt agacgaggta actgctgaag gtgctttcaa taaatggcta gaacctgtat
     5281 ctattgaaga ttataataaa ttgaaataag aagctcaata tacagagtag ataggcactt
     5341 caaatagaag tgtcttttgt gtgccataaa ataaatcaaa atattagcaa agaatgtgaa
     5401 aaacaaaata aaataatatg ctatctttaa aataggaggg gtgctatatg gataacaatt
     5461 tgattaccat tgaaaaaact ttagattata tagaaaaaca tcttttagat gagatgttag
     5521 atttaaatgt aatttctaaa gagataggct attcaaagta ccatttacat agaatgttta
     5581 catcagttgt cggatttagt atacataatt atatacaaag acgtagacta actgaagcag
     5641 caagaatgct catttttaca gaaaaaccaa ttattgaaat tgctttaatc tcggggtatg
     5701 acacacaacg ttctttttca acagggttta aaaaaatgtt taagcatagc cctaagtttt
     5761 ttagaaaaca tcaagatttt ttaccactac aattaaaatt tgatattaat aatagaaaaa
     5821 ggttacatgg aggtatggtt ttagatgtta aaaaagttaa ttgtacagat ataaatattg
     5881 ttggctattc tggtaaagta aaaaaaggat ttcatgttat tggaaaatgt tggagggatt
     5941 tgcataaaaa taaagagttg atagaaaaca aggtagactt ggatttttta attggagtta
     6001 atgattatag taattttaaa gaaattgaaa ataatcctac atttacatat attgcaggtg
     6061 ttcaagtagc tacttttggt aaattcctaa tggaatggaa aaattcgtat taccgaaaag
     6121 taattatatt gtttttactt ttattgggaa aaatgaggat agtatgcaaa atgttgtaga
     6181 atatatctat caagaatggt tccttaattc aagttgccag tttaatgaaa ataatttata
     6241 tgattttgta aaatacggag aaaaaactga taagaatggt ttgagtgaaa ttcagttttg
     6301 ggttccaatc atttaaaaga agggattatg tataaagtag gtggatttat ataggcaaag
     6361 ttttataaaa gtatgtattt ataaataatt tatagtctgt aggaaagaaa tagttttact
     6421 ataagattat gataaaatac acaatactat gataaaatag tactaaataa tagatttaat
     6481 tttgcaaagg ataaagtgtt ttaataaaaa tgaatgtaga aaacgaatta cttaaaaacc
     6541 tagatagatt gcatacaaca gagttaggcg ttgtaagaat taagaaaaat ctttctttgg
     6601 agacaaatga tgtagtgaac tggtgtaaaa ctaaaataga atctccaaat gccattatta
     6661 atagaaaggg aaaaaactgg tatataagtg tatatgattg tattataaca gtaaacgctc
     6721 acagttatac aattattaca gctcataaag aaaagaaatg aattgaaaaa tacaaaggtc
     6781 aaaagcattt tccactgttt gtagtaccta tgaagaagta tataaaataa tattactttt
     6841 gaataataat ttaaattttg gaggttgaaa aaatgaataa tactgtaaaa gaaaaattgg
     6901 cagagtcact aacttctgat actactgata ttttacctta tttgccttat ttattacaag
     6961 atttttggga attaggaagt tcaccagaac aaatgttaca attatttaga aagcatatac
     7021 cttttaatga ggatacaaaa gttttggatt tagcttgtgg aaaaggtgct gtatctgtga
     7081 agcttgcaaa agagttgaat atacatatca aaggtattga tttgatacct gaatttattg
     7141 attatgcgag aagaaaggct attgaatata aggttgatgc tttgtgtgat tttgaaatga
     7201 atgatgctaa tagttctgta aaaattgagc acgattatga ttgtgtgatt tttggagctg
     7261 taggggatat tttaggtaat tataaagaaa ctataagtaa gctattacct acaataaaaa
     7321 gtaaggggtt cattttaatt gatgatgcat atgtagtaga taaatctaca aatgaacagc
     7381 ttaattttga gagggtgtat cccacttaca atgagtggtg tatattgttc aaagaattag
     7441 gtctgaaact aattgaatgt ataccatgtt tagaacttga aaatgatgtt gaagaggaaa
     7501 tgaactggat tataactcgt gcaaatgagc taattaaaaa atatccttct caaaaagata
     7561 tgtttgaaca atatgttatt agccaaaaaa atgaatatac tgatttacag caggaccttg
     7621 ttggagcaac ttggatttta caaaaagaat aagataaata ttagactaga taatcaatgt
     7681 tgtagtgaat tatcccatgt ctagtgaata taaataacat aaaaaaccgt caagatgaat
     7741 agtttattcg tgcttgacgg tttttagtat ttgttgaagt aaaattttat ttctttagta
     7801 tatcactttt agtcaatcca tttgatttag cttgagtatt ccattgatga ttccccataa
     7861 gtttatatag gaatctaggg aagttaggtg aaataaaaat agtacttttg ttggaatcat
     7921 ctatatgtat attatcacta agtatatttt ttgaaagttc ttttaatgca attcctaaat
     7981 cttttttagg tcctttaccc aaaggtacgg attctagtga accaaacatc tccccagctc
     8041 caattccaat accttgaacc catgtaaagt tgattttttt gcaccaatga tctatcattt
     8101 gaattgccaa atgattatgt tcaccttcat tgaatccaca gttaactatt gcatacactg
     8161 gaataggttt tttattttgt gtttttaaaa atttttcaca tttctctaaa aaatgaagta
     8221 aatgagatgg aataccatct acataaagag gaaatgctat tattaatcta tcacaattaa
     8281 gtatggcatt aagagcgtca ggatacttag atgtgctatc tactttaaat gtaaaaactt
     8341 tattatcatt ttgaataagt tgttttaatt cagataaaaa atactgtgaa ttatttttat
     8401 ttacttttgg actaccattt atcattacta tattcatatg gattcacctc ttatatcctg
     8461 tatattttca tagaaacaaa cagagtgttc ttttatattg aaatttatag catttccatt
     8521 tactaatttc gtagcagttt ctttttcagc ttctgtaata caatctccat agaatatagc
     8581 ttttaaattt atctgtttat catatctgct tttatgacgc atcatattat cttttattac
     8641 aaagtatgga agaatataat taatgctttt atcaagtacg tttttgacaa aagggctaaa
     8701 ccctccgtaa cagcatctgc ttattataat aattccatcg gattcattta taagtttacc
     8761 catgttgctg taataatctt ttacaacaca ttcagaagga gttttaatcc agcaaccaaa
     8821 acgaccaata catttgtgga tttcttttgt gtttggaagt atttttatat tgtcatcaag
     8881 cttgggtaat aacttttcta attctttgtc agttaaatca tgtattaaaa gcttcataaa
     8941 gtccatctcc ttttattatg tatgtttatt tctaaaaatt atatcttata gatttttcag
     9001 taaatcttta caccaattaa taacaaaatt ataatagtca actccataaa tcattgtggc
     9061 atacatatat gtatattggt caatacaatg attttcttct acatcaattt tttgtttact
     9121 taatttattt agaagtaatt ctacttcagt tattaacatt tttaagtttg tttcaattat
     9181 ttctttgggt agatacttgt aaaagaatat attaacaagg tggtcttgct ttgttttaga
     9241 aaaatttata ggtttcttta accattcgag aaatacttct ttaccagtat tagttatgga
     9301 atatagtttt tttaatttgc taccatctat aacttcgtga tagtgtatat atccttttgt
     9361 ttctaatctt ttaagtgcag gatagatact tccaaagctg gcgtcaaaaa agtacgaggt
     9421 actttctgac attatttgtt ttaagtcgta tccacttaat tctttttcca ttaaaaatcc
     9481 taaaataatg tattctaaca tattacctcc tggtatcaat cagatatata tcgattcgat
     9541 atatttatta ttacagataa taaatattac gtcaagatgc tttttaaaat aagctaaaaa
     9601 gaattaggaa ttattgataa atatgcaaaa aaattaatat aaaaggtaat taaatatcaa
     9661 tgaagattta aaaatagtta aattaaagta agagtctaat atataaaatt tatagtaact
     9721 ttatggttta aatataaact tgtgtgctat tgacaaagta taaaattagc agtaaaataa
     9781 ttgcatgagc aaatagatta ttgcgaacgc aaaaaaatga ggacaataga tattaaagga
     9841 gagaatatga gagagctatt aaaatgggtt tccattataa atagatacaa tacaaattat
     9901 attgacagga aattggcgga tattggaatc aataatagcc aatattttta tgtattacac
     9961 atatgtaata atccaggtat tacacaggac actatatttg agaaaattct tgtgaatcct
    10021 agtaatataa cgagagcttt ggcaaattta gaaaaagaag gctttataac aagagaacca
    10081 ggtgtaaagg ataaaagaac atggcattta tatcctacag aaaaatcttt agaatgttat
    10141 gatgaaatac taaaaataac aaatgggtat gttgaggagt tgttgagttc atttgagaaa
    10201 gaagagcaag agttgtttat gtcaatgtta aagaaagttg catttttagc tattgatatg
    10261 aatgaagaag caaaattgga ggcgaaggaa aatggaagaa aaatataagg agccattgga
    10321 atatgaaaag gtttcaaagt tattaactaa gtacgcaatt cctagtatta tagcaatgtt
    10381 agtaagtgct ttatataaca ttgttgacca aatatttatt gggcaaggtg ttggtgtatt
    10441 gggtaatgct gctacaaatg tcacttttcc actaacaaca atatgtactg caattgcatt
    10501 attattagga attggaggag cgtctaagtg ttcattggaa ttaggtgcta aaaattcaga
    10561 aaaagctaca aaagcagcag gaaattctat atgcttgatg gctgtttttg gaataagttt
    10621 atttatagta gtatcaatat ttttaacacc actattaaaa ttctttggct ctacatcaga
    10681 aatactacct tatgcacaca catatacaaa aattacatca attggattac catttcttat
    10741 aatgtccact ggaatgagta aattgatact tgcagatggg agaccaaaag cttctatgtt
    10801 ttgtatgtta ataggagcag ttattaatac aattttgaat ccattattca tttttgtttt
    10861 taatatggga attgctgggt ctgctttagc aactgttatt gggcaactta tatcttttgg
    10921 aataagtgtt cattatataa agaattttca acatattaaa ttacataaga aaagttttaa
    10981 attagatagt ataatttata aacatatatt tgcgctaggt tcaagctcat gttttaatca
    11041 attagcaatg actgtaacac agatagttat gaataataca ttggcatatt atggggctag
    11101 gtcagtttat ggaagtgaga ttccacttgc ttgtgtcggt atcattatta aagtaaatat
    11161 gatttttata tctataatta ttggtatatc acaaggtatg caaccaatta tagggtataa
    11221 ttatggggct gaaaagtata atcgtgtaaa agaagcatat aagtttgcag tgggaagtgc
    11281 aactattata tctgttattg cattcttatg tttccaattc ttcccaagac aaattacgtc
    11341 tatatttggt aacggaaatg agacttattt tatatttgca gaacattatt ttagaacttt
    11401 cttgttctta acatttataa atggtgttca aatagtatca tcaactttct ttacatcaat
    11461 tgggaaatca gtgttgggac tttttacttc tttgactcgt caaattttat ttttaattcc
    11521 attgatagta attttaccaa tgtttatggg aattgatgga gtaatgtttg caggaccaat
    11581 agcagatgca gcagcggcaa tggtatgtat atactttgca gttcatgaaa tgaaatcctt
    11641 gactataaag caaaataatt tgatgagtaa ccataataag attgcagagg tataagacaa
    11701 tatttaagaa gtatagaaaa ttaatttaag aaatataata aactaattta ggaagtgtag
    11761 aaaattaatt taaagaagta taataaacta atttaggaag tgtagaaaat taatttaaag
    11821 aagtataata aactaattta ggaagtatag aaaattaatc taaagaagtg taataaact
//
LOCUS       DACTGW010000115        11736 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.115, whole genome shotgun sequence.
ACCESSION   DACTGW010000115 DACTGW010000000
VERSION     DACTGW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.115"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            172..1323
                     /locus_tag="I9573_002329"
     CDS             172..1323
                     /locus_tag="I9573_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HAT4796944.1"
                     /translation="MPIKYWLPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSV
                     GILYINFIVFYLFKFIIDKTKLSMKYEILEKEIIHKEELRLSNECYKIIVEQTDSVVF
                     EWNIKENKSFVSQAWTEKFGYNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNI
                     HSQAVYRLKKSNGEYIWCRTSITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAES
                     DSLTNIYNKGTFEKLVEETIVMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTF
                     ADKIQASFGNKDLVGRIGGDEFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFD
                     SSVSIGIARYPQDGTSFFELFKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(2013..2843)
                     /locus_tag="I9573_002330"
     CDS             complement(2013..2843)
                     /locus_tag="I9573_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT4796945.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPITYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKI
                     "
     gene            2916..4643
                     /locus_tag="I9573_002331"
     CDS             2916..4643
                     /locus_tag="I9573_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796946.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLIMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFVKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNREHFEKKRF
                     DRTAIDYSETAISINKVFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALI
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGRKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDVKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEIKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            4680..6542
                     /locus_tag="I9573_002332"
     CDS             4680..6542
                     /locus_tag="I9573_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796947.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKVKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFSSEYDEEVV"
     gene            6875..7564
                     /locus_tag="I9573_002333"
     CDS             6875..7564
                     /locus_tag="I9573_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HAT4796948.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNNCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSIDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            7669..8355
                     /locus_tag="I9573_002334"
     CDS             7669..8355
                     /locus_tag="I9573_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HAT4796949.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKYGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            8372..10279
                     /locus_tag="I9573_002335"
     CDS             8372..10279
                     /locus_tag="I9573_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HAT4796950.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLTSVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETHKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDKKEVNAELTSSVVKT
                     NTDNETENKTTNDSESKNNTDKDKSGNDNNQKLEGPRGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            10732..11421
                     /locus_tag="I9573_002336"
     CDS             10732..11421
                     /locus_tag="I9573_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796951.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
BASE COUNT         4509 a         1205 c         2026 g         3996 t
ORIGIN      
        1 atactgtcag aaattgtagt tacaagtatt tttgttttat ttgtaaaaga aggctttgaa
       61 ataatgcttg aaaataatag tataagagta ttggcgacaa ttttatctaa aattgtcttt
      121 ttgttgactt gtaaaattat ttgtctattt aaaaaagatg tacatttaga tatgcccata
      181 aagtattggc tccctttatt tttaatacct atatttagtt tatttctgtc tgtttctatc
      241 tttgatgtaa gtaaattttt tagtcttgaa agtttaaaat ttctaagttt aatatctagt
      301 gttggcattt tatatattaa ttttattgta ttttatttat ttaaatttat tatagataaa
      361 actaaactat ctatgaaata tgaaatactt gaaaaagaaa taattcataa ggaagaatta
      421 agacttagta atgagtgtta taaaattatt gttgaacaga ctgattctgt cgttttcgaa
      481 tggaatatca aagaaaataa atcatttgta tcgcaggctt ggactgaaaa atttggatat
      541 aacaatgcat gtaagaatat ttttaaagaa attaaagata aagacttagt tcattctgaa
      601 gataaagcta tatttgaagg ttttcttgaa agtattaaaa aaaagaatat acacagtcaa
      661 gcagtatatc gtttaaaaaa atctaatggt gaatatattt ggtgtagaac atcaattaca
      721 agcatttata atgatgaaaa tgaactttta agagttgtag gagtaattgt agacgtagat
      781 agtgatataa aaaaatatga agagttaaga actagagcag aaagtgactc attgaccaat
      841 atatacaata aaggtacatt tgaaaaatta gtggaagaaa ctatagttat gaatacaggt
      901 gataaaaaag atgcattatt cattattgat ttagatgatt ttaaagaaat aaatgataat
      961 tttggtcatc catttggtga ttttgtacta aaaacatttg ctgataagat acaagctagt
     1021 tttggtaata aagacttagt cggaagaatt ggtggagatg aatttgttgt atatatgcaa
     1081 gattatgtta cagaagtaaa tttgcataaa aaagctaaag aattaaatag agtactttcg
     1141 gataattaca ctgatttgag ttttagtttt gattcttctg tcagtatagg tattgcaagg
     1201 tatcctcaag atggaacttc attttttgaa ttgtttaaaa atgcagatag agcactttat
     1261 agcataaaag catcaggtaa aaattcatat tgtttatttg aagaagaact atatgtacag
     1321 tgaatttgaa tttagatttt atgtgagagt aagatttata tatacttgtg gctgtctgat
     1381 ttataaaata tgttactttg tatattttta tatgtttcag taaggttaat taacataagc
     1441 aaaggtgtaa gtaaatttat attagtttaa gtttacttac acctttataa aaataaattt
     1501 tgtaaattta aacagttggt tttataagga tttttattta ttttaatatc ttagatattc
     1561 ctttttgtat tccttagaga aaataaataa tgaaaggagt acagaaaaaa ttaaacatcc
     1621 taatatacca cttgaattaa gtcttaaagc attaaagttt aaaaaagcat tatttaaatc
     1681 ggtatccaaa agaaaaggat gctttgattt gctatatatg gaaaaattaa aaatatattc
     1741 ataatcaaat ttataaaatt aatatctttc catcctaaca ttacatttac ttagattaat
     1801 tattattata aaaataaaaa tacaaataat agattcattt acattcttaa atgattaaag
     1861 ctctttaccc tttaaacatg cataacatat cgttataatt ccagtaaata agactaaaat
     1921 gcccattcct aaaattgcag tttcatcatc tattactcca aatgctttta aagtaactta
     1981 tttcgctttg tataataata atttgtctct ttttatattt taacttctgc tgagatttga
     2041 gttagatatt cgttagaatc tcgcacactt atatcatata gaagatattc ttcataaaca
     2101 aaatccccaa tacgtaaatt attctgatta aaaaaattta ataacttttc ataagccttg
     2161 taaatagttt cataactacc tttatgataa gcagttacat aaagtccctt tggtttgatt
     2221 gatacaggat atttcttgta ctctttatta acttttgtaa ataaatatga atatgattca
     2281 aagtctttat taattatatc ttcaccttta atgattgagc caatcgtacc atagtcactc
     2341 aattcatagt tgtccataaa gttaatacat tcatataaaa aattttcttc ctcatctcct
     2401 aaaaactgtt ctttatatgt aataggtgtt ttgaccaagt actcttcctc atgttctttt
     2461 aaagtaatag tattcgcatc tatattacag gcatttttag tgaatgaaat agtagattcc
     2521 attagagctt gaatattatt tagtttttca atttcatttt taatatcaat ggttttcttt
     2581 tctaaaagct caattagtaa attaggtgtt cttttgtcta tataagcttt aatttctttt
     2641 aaaggcattt ttacttccct aagtgaagaa attataccaa aaatgctaag ttgatggtat
     2701 gagtagtatc tatatccatt ttctttcttt aattctggtg agaaaagacc tatatcatca
     2761 taatggaaaa gtgttttttt attgattcca caaatctttg caaattctcc agttgtaaaa
     2821 taattttttg aaacttgact cataattata attcctcctt gactatcctg ttacagtata
     2881 gtttatcata gtattttgta aaggagtgtg attatatgaa aaaattaatt cattttttaa
     2941 aaccatatag agttttaatt gttgtagttt tgatatttac gtttttacaa acacttggaa
     3001 ctttgtatat accaacactt acagcaaata tagtaaacaa tggtgtagta aagggagata
     3061 tagattatat tgtaaaaaca ggtttaataa tgatgatagt agctggtata acagctttat
     3121 cagctgtgtt agtttgtaaa gtatcagcaa atctttcatc tggattttgt agagatatta
     3181 gagaagcagt atttgtaaaa tcacaagatt tatcaataaa tgattttaat aatataggta
     3241 cagcatcaat gattactagg agtactagtg atattacttt aataggtcag tctgtgttta
     3301 tgtttattca attggtatta ccagcaccaa ttataactgt ttctggtcta ttcttagcat
     3361 attctataga taaagcaatg acaattatta ttgttgtagt aatgttttta ttcatgcttt
     3421 cagcattttt agttggtaaa aagcttataa agttgtttaa aatgatgcaa ataaagatgg
     3481 acaatatgaa tagggtatta cgtgaggtag taactggagt aagagttata agagctttta
     3541 atagggagca ttttgaaaaa aagagatttg atagaactgc aatagattat tctgaaacag
     3601 caatttcaat aaataaagta tttgcagtac ttatgcctat tgtaatgtta ataatgaatt
     3661 taggtatagt aagtattatt tggtttggtg ggatgagagt cagtaatgga aatatggaga
     3721 taggtcatat aatggcactt attgaatatt gtatcttaat tttattctat ttaatcatgg
     3781 gagtaatggt atttatgtac atacctagag caggtgcatg tgcagataga gtaaatcaga
     3841 tattagatat agaacctgaa attgtagatg gaaatggtcg taaagatact gtctcagaga
     3901 ggggacattt agtatttaaa aatgtaacat tttcatatgc acaatcagaa gaacctgtgt
     3961 taaataatat aacatttgaa gcaaaatctg gagaagtaac tgcaattata ggaagcactg
     4021 gctcaggcaa atctactatt gcaaatataa taccacgttt ttttgaaatt caatctggag
     4081 aaatatctat aaatggtcag gatgttaaga agattcctca aaaagaactt agagataaga
     4141 ttggatttgt gccacaaaaa gcttttttat ttagtggaac aatagaagaa aatataaggt
     4201 atggaaaaga agatgcaagt attgaagaaa taaagcatgc tgcaagtata gctcaagctg
     4261 atgaatttat ttctgatatg gaagacaagt atgactcatt tgtagcacaa ggtggaaata
     4321 atttgtcagg tggtcaaaaa cagagaatat ccattgcaag agcgttagtt agaaagcctg
     4381 aagtatatgt ctttgatgat agtttttctg cattagattt taaaacagat aaaagactta
     4441 gaaaagcgtt gaaaaatgaa atcaaagatt catcagctat aattatagca caacgtataa
     4501 gtacaatcat ggatgctaat cagattatag ttttaaatga tggaaaaatt gtaggtattg
     4561 gaaaacataa agatttactt gaaaattgtg aagtttataa gcaaattgcg gattcacaac
     4621 ttagtaaaga agaacttgca taaaatgaag aattaatctg atatttggag gtgtatgata
     4681 tgagtgaaaa aagaatagat gaacaagata tagaagtact tagttttgaa gatggcatgt
     4741 cagcagaaaa agttaaggat gcaaaaaaaa ctactaagag attattaaaa tatatggcaa
     4801 aacaaaagtt taaaatatta attattttta tttcagtatt gataagttca gcgcttacag
     4861 ttttagcacc tatggttatg ggaaaagcta ttgaccaact atttaatgga ataaaaactg
     4921 cagtccaaac aggtacaaaa ttcagtgtga attttagtac tatggggggg atagtatcaa
     4981 tacttctagg gttgtactta attagtgcag tatttatata tattcaacag tatattatgt
     5041 caggagttgc acaaaatcta gttcttagca tgagaaaaga tttaagtgat aaactcaata
     5101 aacttcctct taagtactat gattctcata aaaaggggga aacacttagt atagtaacaa
     5161 atgatttaga aaaagtagca gatagtttac aagaaggatt aatgcaactt attacagcag
     5221 tagttacagt agttggttct attgtaatga tgataagtat aagtgtacca cttacaatag
     5281 tatcagctat aacattactt gtttcattgg gtattactgt tgtaattgca agaaaatcac
     5341 aagaaagatt ctctgaaaat caaaaggcac taggagagtt aaatagcaat atagaagaga
     5401 tatttactgg tcaaatagtg gttaaagcat ttagtaaaga aaaggatact ataagaaact
     5461 ttaaaatatt aaatcaaaat ctttataatg caagtagaag agcacagttt tcgtcatatg
     5521 caatatctcc aattattaga tttataaacc taattggcta tgttattata gcagttgtag
     5581 gtgggatatt tgcatcaact ggagcaatga ctcttggtag tatacaagcg tttattcaat
     5641 atgtaaatca agcatcagag cctactacag aaatatctta tattgtaaat atgcttcaag
     5701 cagcaatagc atctgcagag agagttttta cagttatgga tgaggtggaa gagataaagg
     5761 atgaagaatc ttcaaaggtt attagcatgc ctaaaggtaa agtacaattt gagcatgtta
     5821 aatttggata tagtgatgat tttattctta tgaaagatat taatatcaat ctaaatgcag
     5881 gagacaagat tgcaattgtt ggacctacag gagctggaaa gacaactctt gtaaatttac
     5941 ttatgagatt ttatgagata caaggtggaa gaattactat tgatggtgtt aatataaaag
     6001 acttaaagcg tggagaactt agaactatgt ttggtatggt tcttcaagat acatggctgt
     6061 ttaatggaag tataaaagaa aatattgcat atagtaagtc agatgccaca atggatgaaa
     6121 ttgtaagagc agcaaaatca gcaagagtag accattttat aaggacttta ccacaagggt
     6181 atgacactat attgactgag gatgcttcta atctatctca aggtcaaaag caacttctaa
     6241 caattgccag ggcaatactt tcagacccat cagtattgat tcttgatgaa gcaacatcta
     6301 gtgtagatac tagaacagag gtagaaattc aaaaggcaat gaataacttg atgaagggaa
     6361 gaactagttt tgttattgca catagattat caactattcg agatgctgat ttaatattag
     6421 taatgaaaga aggcactatt attgaacaag gaagccataa ggaacttatt gaaaagaaag
     6481 gattctatga agaactttat aatagtcaat tttcaagtga atatgatgaa gaagttgtat
     6541 gattatcaag ttttaggcaa ctaaatgatt gataaatatt aagttaaata tagtaacatg
     6601 taaggttata tggaataaga gtatttctct atttcatata acctttttta tttatcaaaa
     6661 taagataaaa ttttctttct aaattagatt gaagaaagta tttatcaata taaaagtaaa
     6721 ataatcatta ttattagtga agtaaatatt gtttgccaca tatatgccat aaagatgcac
     6781 ttattttacg gagatttaac aaagtagtgt aaagtatact ctaaatataa aataaatcaa
     6841 atagagttaa ataaattgag aggtgatata aatagtgaat aaaaaattga actatagatt
     6901 tgaaatgaag cacaaaataa ctgaagcaga tgtgttagct ttaaaatcaa gactctatcc
     6961 aataatgaag aaagatgaaa atgcatctaa agatggaaaa tatcttatta ggagtttata
     7021 ttttgatact cctgaagata aagcactttt agataaatta aatggcgttg caataagaga
     7081 aaagtttagg ataagatttt ataataataa ctgctcttat ataaggttag agaaaaaaat
     7141 caaacattac aacatgacat caaagctatc aactaattta accaaaaaag aagtaataaa
     7201 tatactaaac aatgatattg aatttctaaa agagtcgaca aattgtttat taagagaatt
     7261 ttatttgaaa ttaaaatgtg aaagattaga ggcaaaaagc atagttgatt acaatagaga
     7321 agcttttatt tatccagttg gaaatgtaag agttacaatc gatagtgata ttaaaacttc
     7381 tgtaaattcg atagatttat ttaataaaga tttgccgact gtttcggtaa ttgatgagaa
     7441 tatgactgta ttggaggtta aatatgatga atttatacca gattttataa aggatttaat
     7501 tcaaattaat aagactactt ctacagcagt ttcaaagtat gcttcaagta gattatatat
     7561 ttaagaataa aggctatgat ttatataaag atatacataa ataaggttaa aaaacaaata
     7621 ggttaaaaat aaataaaata aaaaataaat aagttaaaaa taggaaggat gattttaatt
     7681 atgacattta atgatatttt taaatcaagt tttatagaaa acgtatcagg tttttcattt
     7741 gtggacagtg ctttagcact aggttcagct tttttagtag gtttgtttat atatatggtc
     7801 tataaaaaga cttatatggg aattatgtat tctcgaccat tcaatgtgtc gcttgtagca
     7861 cttacaatgc ttacaacttt tgtaatattg gcggttactt caaatgtagt tttatcctta
     7921 ggtatggttg gtgctttatc tatagtaaga tttagaacag caattaaaga cccaatggat
     7981 ttagtatttc tattttggtc attgggttca ggtatagttt taggagcagg tttgattccc
     8041 ttagctgtca tgggttcaat aattatggga ttgattctaa tattcttttc aaataaaaca
     8101 atatctgaaa ctccatatat attgatggta aattgtaata atgaagattc agaagatata
     8161 gcaactgata aaattaaaaa agtatttact aaatatcaaa taaaatcaaa aagtgttaca
     8221 ccagaaaaag gaatagaatt agtgtttgaa gtaagaatga aatatggaga gacaagtctt
     8281 ataaatgact tgagtcaagt agatggagtt actaatgcag ttcttgtaag ctataatggt
     8341 gactatgtag catagagagg agtaatcttt tatgaaagat aaaaaattta cccttcttat
     8401 ctctattatg attatatttt tatgtgctgt agttggagtt tatagtacat ctagcaacaa
     8461 aagtgttgat ttatatagtg atgtatatat tgaaaaatat tttaacagag acaaggttat
     8521 ggaagttaat atagagatag atgaaagtga cttgaaggat atgaatgaaa atgctataaa
     8581 agaagaattt aaggttgcaa aagtaactgt agatggagat acatatggaa acgtaggtat
     8641 aagaactaaa ggaaattcaa gtcttacatc tgtagcaaat agtgatagtg atagatacag
     8701 ctataagatt aattttgata agtataatac tagtcaaagt atggaagggc ttactcaatt
     8761 aaatcttaat aactgttact ctgacccatc ttatatgaga gagtttttaa catatagtat
     8821 ttgcgaggaa atgggattag cgactccaga atttgcatat gctaaagtct ctataaatgg
     8881 cgaatatcat ggtttgtatt tggcagtaga aggattaaaa gagtcttatc ttgaaaataa
     8941 ttttggtaat gtaactggag acttatataa gtcagatgaa ggaagctcgt tgcaatataa
     9001 aggagatgac ccagaaagtt actcaaactt aatcgttgaa agtgataaaa agacagctga
     9061 ttggtctaaa attacaaaac tattaaaatc tttggataca ggtgaagata ttgaaaaata
     9121 tcttgatgta gattctgtcc ttaaaaatat agcaataaat acagctttat taaaccttga
     9181 tagctatcaa ggcagttttg cccataacta ttatttatat gagcaagatg gagtattttc
     9241 tatgttacca tgggatttta atatgtcatt tggtggattt agtggttttg gtggaggtag
     9301 tcaatctata gcaattgatg aacctacgac aggtaattta gaagacagac ctctcatatc
     9361 ctcgttatta aaaaatgaga cacacaaaac aaaataccat aaatatctgg aagagatagt
     9421 aacaaaatac ctagattcag actatttaga gaatatgaca acaaaattgc atgacatgat
     9481 agcatcatat gtaaaagaag acccaacagc attttatact tatgaagaat ttgaaaaaaa
     9541 tataacatct tcaattgaag attctagtga taataaggga tttggtaata aagggtttga
     9601 caacaataac tctaataaca gtgattctaa taataattct aatagtgaaa ataagcgctc
     9661 tggaaatcaa agtgataaaa aagaagttaa tgctgaatta acatcaagcg tagtcaaaac
     9721 taatacagat aatgaaactg aaaataaaac tacaaatgat agcgaaagta agaataatac
     9781 agataaagat aaaagtggaa atgataataa tcaaaagcta gaaggtccta ggggtaaagg
     9841 aggtaagtca ataccagggg ttttggaagt tgcagaagat atgagtaaaa ctataaaatc
     9901 tcaattaagt ggagaaactt cttcgacaaa gcaaaactct ggtgatgaaa gttcaagtgg
     9961 aattaaaggt agtgaaaagt ttgatgagga tatgagtggt atgccagaac cacctgaggg
    10021 aatggatggt aaaatgccac caggaatggg taatatggat aagggagata tgaatggtaa
    10081 aaatggcaat atgaatatgg atagaaatca agataatcca agagaagctg gaggttttgg
    10141 caatagagga ggaggctctg tgagtaaaac aacaacatac ttcaaattaa ttttaggtgg
    10201 agcttcaatg ataataatgt cgattatgtt agtaggtgta tcaagggtaa agagaagaag
    10261 atttataaag tcaaaataag attaaaattg aatataaata attttaatac tgttaagtct
    10321 aaatatttac ttagcagtat ttttttagaa aataatgtgt ttgattaata atataaggta
    10381 ataaattgat aaaaattaaa tacagcatat taataataca taataaagta ataaaaaaac
    10441 tgtttaagta taataactta tatttagaca gtttttttca tttataaata atataaatga
    10501 aattgaaaac atttgaagga tagaaattgg ttggataaat gtataaatta cttcaccaaa
    10561 aaataatgac aagagttgta actaagttgt aataaaatgg tagttattta gcataaaata
    10621 atgtatcata gaagtatatt ataaaattaa taaaataaga tttttgtaag aaaactgctg
    10681 gcttttgata tttagagtga gtagttaaat tatgtttagg aggggtagaa tttgttgcaa
    10741 aaagtattat ggaaattttt aaagctagta gtcggtttat ttatatgttc agttggtata
    10801 gtattgacga taaattgtaa ccttggatta tctccctggg atgtatttca tcaaggactt
    10861 tcaaatcata taggaataac tataggaact gcttctataa ttgtaggctc tatagtagtt
    10921 attgctgatg tcgttcttgg agaaaatgta ggatggggaa ctgtttttaa catgctacta
    10981 attggattct ttatggattt acttttatat agtaatttaa ttccagaggc tgatagttta
    11041 tttgtaggaa taattatgtt gatattagga ttggttcttt tgtctgttgg aatggtattt
    11101 tatatgggaa gtggtttagg aagtggacct agagatggtt tgatggttgc aattcaaaag
    11161 aaaacaggaa aatcacttaa attaataaga ggtactatag aagttggagc attaatagta
    11221 ggatttttat taggtggaaa agttggtata ggaactataa taagtgcatt tggattgggg
    11281 tatttcacac aaatggtatt tggcttattt aaattagatt gtagtaagat taaacataga
    11341 tttatagtag atgatataaa atttatcaaa gtgtatatga gtggtgagaa aaaagctgct
    11401 gttcaaaata tagttaagta atttaagaat aaattcaacg aaatgttaag tattttaaat
    11461 taacatttta tttagatgga taaattgaat attaaaagaa tattaaaata tgaggattgc
    11521 tagaacacta taaaatatag atttctagca attttttatt tgttcaatgt ttttttagta
    11581 ctagaagtct tattctctat ttaatgaatg aattaattta taattagaga tactaaactt
    11641 tatagatatt aatataaaca cctttaaact gtgtattttc caaagccttt aaaatgcttg
    11701 tatttattct aaataaaaaa ataaaaaaat gtcaaa
//
LOCUS       DACTGW010000116        11736 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.116, whole genome shotgun sequence.
ACCESSION   DACTGW010000116 DACTGW010000000
VERSION     DACTGW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.116"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(145..1725)
                     /locus_tag="I9573_002337"
     CDS             complement(145..1725)
                     /locus_tag="I9573_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAT4796952.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            2523..3424
                     /locus_tag="I9573_002338"
                     /pseudo
     CDS             2523..3424
                     /locus_tag="I9573_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            3411..4166
                     /locus_tag="I9573_002339"
     CDS             3411..4166
                     /locus_tag="I9573_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT4796953.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTSPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            4297..5319
                     /locus_tag="I9573_002340"
     CDS             4297..5319
                     /locus_tag="I9573_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT4796954.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRIGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(5483..6040)
                     /locus_tag="I9573_002341"
     CDS             complement(5483..6040)
                     /locus_tag="I9573_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAT4796955.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(6046..7122)
                     /locus_tag="I9573_002342"
     CDS             complement(6046..7122)
                     /locus_tag="I9573_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAT4796956.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(7123..7806)
                     /gene="modB"
                     /locus_tag="I9573_002343"
     CDS             complement(7123..7806)
                     /gene="modB"
                     /locus_tag="I9573_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAT4796957.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(7955..8767)
                     /gene="modA"
                     /locus_tag="I9573_002344"
     CDS             complement(7955..8767)
                     /gene="modA"
                     /locus_tag="I9573_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT4796958.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKTDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEVESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            8960..9889
                     /locus_tag="I9573_002345"
     CDS             8960..9889
                     /locus_tag="I9573_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4796959.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(10050..10499)
                     /locus_tag="I9573_002346"
     CDS             complement(10050..10499)
                     /locus_tag="I9573_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796960.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARVLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(10702..11682)
                     /locus_tag="I9573_002347"
     CDS             complement(10702..11682)
                     /locus_tag="I9573_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT4796961.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVICGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         4016 a         1928 c         1383 g         4409 t
ORIGIN      
        1 gttagcttta ggggatttta gcaattcaag tagtattgga taggttctat cctatctcta
       61 catataggtt tccctatatg ctgactattt tagctactaa ctcatgacta aagtcacaag
      121 tgtgcgtagc tattttttaa tcaattatat atccaacaac tctttcattg ttaatgaaat
      181 gttacttatt aaatccgtag catttaccgt atacattgct ttgctcaatt tatctcctat
      241 atagccatgt atatatgctc cactaactgt agcttccaat atatccatac cttgacctat
      301 aaatgaagta atcattccaa gcaaacaatc acccattcca ccagttgcca tacagctatt
      361 tcctgtagga tttatatatg tttttttacc atctgttatg acagtttgat aaccttttaa
      421 aagcaccacc agattatatt tttgagcaaa ttcttttgca acatctactc tatgttctct
      481 tatataccca attgaatctc ctgtcaacct ggccatttct cccaaatgag gtgtaattac
      541 aaatctattt ttccattcta aaaacttata acatctgtcc ttcataacgt tcaatccatc
      601 tgcatctaat actattgggc aattagaatt ttctactatc ttaagtaatt tatcaaatgt
      661 ttgactatta tctcccatac ctggaccaaa accaattgca tcactagaat taagtaattt
      721 atttaatctt tcttcttcaa aatttaaagt cattgcttct gtaagtctaa tagatacctt
      781 ttcttgcaca tcttcactac taattaatgt tacaagtcca ctcccactct taacagcaga
      841 ttcagttgct atgaaacttg caccataaaa tccttttgaa cctgcaacta tagatacttt
      901 tccaaaatca cttttaaaac catgcttatc tcttcttcta atattatttt ttacaaaagt
      961 cttatcagtt atgaactctt catcatggta ttttcttgta ataaaatctg gaacacctat
     1021 cttttctaca actacatcac ctataagtgt gctagtgtca tagtttagaa aacctctttt
     1081 ataaaactca aagctaatcg ttttgtttgc tctaatacaa gttcctaata catttccatt
     1141 atcgctatct atacctgaag gaatatctat agaatatgta tttttgctat tatcgtttac
     1201 tatgtcaata acttctttaa aaataccagt tatatcacgt tttaatccag tcccaaatat
     1261 agcatctact acaacatcac ttttagatac tctatcttta aaaattgtta aatttttatt
     1321 ttttataata ttttgtttct caactttaat aattttatct acttcatttt catctccata
     1381 atttttttca cttaaatcat ttttattaat atttataata ttaattccca tatttaaaag
     1441 tacattgtaa tttattttag agcattcact tagtttttca agattaccta ctaaaaatac
     1501 gtttacttct tttccatatg tatttagatg tcttgctatt ccaagaccat ctcctccatt
     1561 atttccaaca ccacatacaa ctgtataact attatacatg tctatatcca tatttttcat
     1621 tgcagatata accgcatttt ccatcaatac aatcattgga atccctactt cttctacaca
     1681 tgcagtatca acatttctgc ttaatttaga tgtacctatt agcataaatt atcactccta
     1741 taaagttaaa gacttcttaa atacttacca aaaatatcta ttgaaatctt ttaatcccat
     1801 aaatatcata aataaaatca ctaaaactaa gagttttgaa ataatttaaa ataacaaaaa
     1861 attttgaatt aaaacaatta tttaatttat tataccatat aatttaagtt attatgttga
     1921 tttaaaacct atttacacta attatctaaa tatctcttta tttttataat cttttatgac
     1981 ttatttatat ttttcatttt tataaaaaaa caataattaa gaatataact atattctaca
     2041 tgtaaaaatt tttttattta aactcaattt tctgataact tgataatttt tcaaaattta
     2101 tagttgactt ttatttttta ctgtattaaa ctaattacta tacaaaacag aaaacaaaat
     2161 tttcaaaatt attaaattaa aaggagaggt aaatatgaag actttattaa acaaacaagt
     2221 acgatttttt agtagccaat ttagttaggg tttgtaccta agttaagaca aattgtcata
     2281 acatagatac aagcccctta ggattatgat ttttaaggat tgtatctatg ttggctatag
     2341 taattgttgt taaagttttt attttatcat cataatatag agccacatgg attatactgt
     2401 gtggcttatt cgaatttttg ccatacagtt atatatactg tgtggttttt ttatatcaaa
     2461 atcatttttt aaaaccaaat ttaaaaaacc atttaaaata ttttcaaagg ggtgttttat
     2521 ttatgtcagg tattatatct gtaatgacgc aaagtttaat tttatcgatt atggcactgg
     2581 gagtttatat aacttacaaa atcctagatt ttccagacat gtctgctgat ggtagctata
     2641 caatgggtgc ttctattgta gcattttctc ttactaatgg tatttctcct gtagtagcaa
     2701 ctttaatggc tatcttatgt ggttgtactg ctggtcttgt tacagggatt cttcacatta
     2761 agtttaagat atctaactta ctttctggta ttttagttat gggaatgcta tactctatca
     2821 atttaagaat aatgggaaaa tcaaatattc ctctatttag ctttaaacac ttattcaatg
     2881 gggaaatatc tcctatagtt ttagccttag cttttgtatt catctgtaaa gtccttttag
     2941 atttattcct aaaaacaggt cttggataca ctttaaaagg tgttggtgat aactctcaaa
     3001 tgattaaatc tctgggtata aatataggtt ctataaaaat actaggactt atgatttcaa
     3061 acggacttat agctttatct ggtagtctta tggctcagtt cttgggattc tcagatgtaa
     3121 atatgggtat aggaacttta gttcttggaa ttgcctctat cataattggt attacactat
     3181 ttaagaaatt tacttttatc aaagatacta ctgcaataat tgttggctct tttatatacc
     3241 aattcactat atactttgca atgagtttag gaatgttatc aactgactta aaactaataa
     3301 ctgccatagt tataatagct ttcctagcta ctggtaactt aaatatttca ctaaaaaaaa
     3361 caaatgcaaa gctagtacca aaaataaatc agaaaaaaga ggtgttgtag atgttacaaa
     3421 ttaaaaatct ttcaaagagt ttccctaatc catatggtga acctaatact atctttgaaa
     3481 atttatcaat cgatatagaa gatggtgaat ttgtaagtat cataggtagt aatggaactg
     3541 gtaaatctac tttattaaat ataatatccg ggcttataaa agaatcttct ggtcaagtat
     3601 tattaaataa acataacttg tctaatttag ctgaacacag aagaactcaa atagtaagta
     3661 gagtttttca agacccaagt cttggaactt gcccttctat gactgttaga gaaaacttgt
     3721 ctttagctct taacaaagga aaactattaa atctaagaaa atgtcttcgt tacaaaagag
     3781 atttcttaga aaatttattg gaaggtgtat ctcttgattt aaaaaaatac cttgatgttc
     3841 aagtaaaatt tttatcagga ggtcaaagac aatcattatc actaattatg tcgtgcttaa
     3901 caagtccatc agttttatta cttgatgaac atacagcagc acttgaccca aaaacatcaa
     3961 atgaagtaat tgaattgact aataagattg taagggaaaa aaatataact acacttatgg
     4021 ttactcacaa cttaaaacac gctcttcatt atggtgatag attgataatg cttcataagg
     4081 gtgaagttgt attagatgta aaaggtaaag aaaaagaaca attaacagtt gaagaaatct
     4141 tagaaaaatt tgaatatgca gtttaaaata atatatctat tgtattaaac tcggctttaa
     4201 aataaagtta aaattacaat aattatggtt actttgtaat aattcatatg aatatatgaa
     4261 catatataat aataaatttt tgagggggat ataaaaatga ttaataaaaa aagattggca
     4321 agtttgattt tagctggtgc tcttagtata tctatgctta cagggtgttc acaaggagga
     4381 gactctggta attcaaaaca agaaagtaat tctaaaggta aagaagttaa aaaaattggt
     4441 ataactcaac tggttgaaca tccagcatta gatgccacta ggataggttt tgtaaaagca
     4501 ttagaaaaga acggatttaa agatggagaa aatattgata tagacttcca aaatgctcaa
     4561 aatgacatgc ctactacaca aagtattgcg agtaaatttg catctgataa gaaggactta
     4621 atatttgcta tatctactcc atcagctcag gcagcattta atgctactaa agatatacct
     4681 atacttatta ctgctgtgag tgaccctgtt gctgctggat tagttaaaac tcttgaaaag
     4741 ccaggcacaa atgtatctgg aacatcagat tttgtttctg tagacaaagg tttagaactt
     4801 cttaaaatat ttgctcctaa ggcaaaaact ataggagtta tgtacaatac tagtgaagta
     4861 aattccaaag ttcaagttga tgctttaaaa gaatatgcat ctaaaaatgg atttaaagtt
     4921 gttgaaaaag gtataactac ttcaaatgaa gttaaccaag gtatttctag tttagttggc
     4981 aaaatagacg tattatatgt tcctacagat aacttagtgg cttcttctat gcctatagtc
     5041 tctaaaatag ctactgagaa taaaatacct gttatagcag ctgaatctgg gccagttgaa
     5101 aaaggtgctc ttgcttgtca aggtatcaac tacgaaaaac taggttacaa aacaggtgaa
     5161 atggcagtta aaattttaaa tggcgaatca gtatctgata tgcctgtggc tacatcagat
     5221 gatacagata tcattgttaa tgaagatata ttaaaagctt taggtatgga aaaaccttct
     5281 aatgaaaata tttcttatgt aaagactaaa caagaataga aagatatttt ctatatacga
     5341 ttaatatgcc tgtttctata aattaattta tagaaacagg catatatttt tattttaatg
     5401 tattatctat ttaaagatta tatgtaaatg ctcttaatat gattgtataa attttacttg
     5461 tataaacttt atacaatcat atttattctt ctattttctt tattactcta catggatttc
     5521 caacagctac agtatttggt ggtatatctt ttgtgactac acttcctgca cctattacta
     5581 cattatcacc tatagtaatt cctggtgtta tgattactcc cccacctatc catacattat
     5641 cacctatttt tacaggagaa ccatactcta tgcctgaatt tctaagttgt gcatcaattg
     5701 gatgataggc tgtatagatt tgaacgtttg gagctaacat tacattgtca cctatttcaa
     5761 ttttacatac atctaaaaaa atacaatcat aatttgcaaa gaaattttcg cctacatgta
     5821 tgttatatcc ataatcacac ctaatgtttt gttctacatt tatttgcttg ccaactgaac
     5881 caaatagttg tcttaaaata tcttctcttt tttcatattc atcctctaaa gtattattaa
     5941 acaatctagt taatttttta caatactctc tttcttttac taacaattca tcatttgcat
     6001 agtatccttt tccagacaac attttttctt tttcagtcat atagtttact cctttttgtt
     6061 ataattgtat ctaaagcaaa ataaatattc ctgtggaaaa tctaaatata ttctgtcacc
     6121 tttcttaaat ctcattttac ttttttccaa ttctatttga ataggtacac attcgttaga
     6181 taaatcagtt tttctaacat atatagtata tgtaaaagga ttttctacaa tgttaatcaa
     6241 ttcgaaacat aacttttcat ttaaatcttc attagaatcc aagaccttaa tatgatgctc
     6301 cctgattccc acatattcta tattatcacc tatttctcta tttaatgtca actcgcatcc
     6361 ccagtctgta gcataaatag tatttttatc cagtctattt aatttggata tatttttaca
     6421 ccctgttatc ttagcttcta taaggtactt tggagattca aagagctctt ttttaggtct
     6481 ctttggaaga ccttctcctt tgttgtacac tataatatca tcacaaactc ggtaagcttc
     6541 ttcaatatca tgagtaacaa atagtatatc tcccttgtaa tcttttaaca tattcataag
     6601 ttctttttcc atattacttc ttaaatgatg gtccagtgct gagaaaggtt catctaaaag
     6661 taatatatct ggagatgttg ccaatgctct agctagtgct accctttgtt gttgaccacc
     6721 agataattgc caaggatatc tcccttcaaa cccatttaat ttaagtatat ctaagtacct
     6781 tgctactatt tccttttttt cactcttact tagttttaaa agcccaagtt ctatattttg
     6841 acttacagtc atatgtggaa acaaagcata gttttgaaat aaaaatccta cctttctatc
     6901 ttgtgttgat aaatttatct tcttttcaga atcaaataga actctatcat ttaatacaat
     6961 tttacctctt gttggtttct caagccctgc tatacatttt aatgtcatac tttttccaga
     7021 accagattcc cccaaaaacc ccagagtacc tttttcttgt ttaatttcta cctttagttt
     7081 aaaagaggat aaatcttttt caatatccac gtataagctc aactatccac acctctttcc
     7141 tataattttt tgctgaaatt ccgaccaata atttagtaat aaaatcattg ctatagatat
     7201 tgctacaata atcataaccc acatcatagc tttattcata tctccgcctt caacagcaaa
     7261 aaatatagct attggcatag tttgagtttt tccaggtata tttccagcta tcataagtgt
     7321 agcaccaaat tcgccaagag ctctggcaaa cgacagcacc aatccaccaa ttattccagg
     7381 ccatgctaat ggaactgcaa tcttaaaaaa tactttccat tcattaagac ctaatgttct
     7441 agcagctgac agcatattat tatcaatctg ttcaaaagct gaccttgaag ttctatacat
     7501 cataggaaaa gacaccacta ctgctgaaat tacagttgct gtccaactaa atattaaagt
     7561 cctatcgaaa tttaataaaa actttcctat aaatccattc ttcccacata gtaataataa
     7621 aaagaaacct acaactgttg gtggcaaaat tagtggaagt gtaaatagtc catctataag
     7681 acccttccac ttacctctat agttagccat aatatatgac acaaatattc ctattacaaa
     7741 agttattatt gttgacaaaa tagatgtctt aagtgatatc cataagggtg accaatctgt
     7801 tcccatcttt ccctccttaa ttttaaatgg aaatagtgaa actaataact ctattaattt
     7861 cactatttcc attttattat aatacacaat tattttcaat tttcatttat ttatatataa
     7921 tttatactta attacacata atttatttat aagtttattc tactttttta tatccaaatt
     7981 cttcaaaaat cttttgtcct gactcactta ataaaaattc ttcaaatttc ttagcagcat
     8041 ctacgttttt actagcttta attactgaaa ctggatatgt aataggtgaa tgagtttttt
     8101 cgtctgtttt ttcaactact tttatcttat cattattgac agtatcactg aagtaaacga
     8161 atccaacatc agcatttcct gattgcaccc atgctaaaac ttcttttaca tcttttgcaa
     8221 atactagctt atcttttaac ttatctttta aatttaaatt agttaacacc tcatctgcat
     8281 acttaccagc tggaacactt tcaacttcac caacagcaat tttcttcact ttatctgtag
     8341 tcaaatcttt catacctgat acactactgt cagcagaaga aataagaact aaatcatttt
     8401 ttactaaatc tttcatagta tcagaaacta ataacttctc ttcatccaaa gctttcattt
     8461 gtttttgacc tgctgaaata aataaatcac aaggtgctcc ttgttctatt tgttgttgta
     8521 atgagcctga tgctccatag ttaactgtaa gttttacatt tgaatcaact tttttatatt
     8581 cttcttcaat ctttgccatt gcttctttta aacttgcagc agccgatata tttaactcta
     8641 ctgaatcact acttttagtt gttttttctt gttttccatc atctgttttg tcttcattac
     8701 tattgcaccc aactgtcccc aaagttaaac atgcaactaa tcctaatatt cccaatattt
     8761 ttttcataac aatctccctt tttgtaaatt tgatacacat tttaatatgt tttattatgt
     8821 tttatcatgt tttgttatat atagtttaat tatatataca tgataataaa ttttcaagta
     8881 atttatttac actactttta aatattttgc tacattttcc tttttttaat gttaaaataa
     8941 ccttatatgg aggtggatta tggactcatt atcatctttg acacctgtcg aggtagctga
     9001 attattaaag ataacaaaaa atactgtata tgaattaatc aaaagaggtg aacttccttc
     9061 ttataaagta gggaaaaaaa taagagttga tactaaagat gttgaagaat atatcaacag
     9121 ccaaaaaact ggtaaagtaa aggatacaca tttcaacaat aaaaatatag atataaataa
     9181 taatttcaaa attattattt ctggtcaaga tgtaatatta gacattttag ctaggtctgt
     9241 tgaaaaaaaa ttggatggaa taagtacatt tcgctcttat ataggtagtt ataatgcttt
     9301 gtatgagtta tataatggca gagtttctat agcatcatcc catctatggg atttcgaaac
     9361 ggatgaatat aatagcactt ttgttaaaaa gctattgcca ggaataccat gtgtacttat
     9421 aaatctagct tatagaatgc aaggtttcta tgttgctaaa ggaaatccta aagagataac
     9481 aacctgggag gatttaacaa aaccaagtat aaccattgta aatagggaaa aaggttctgg
     9541 aactagggtt ttactagatg gtaaacttag acttctaaac tttaatggta aacatataaa
     9601 aggttatgat aatgaagaat tatcacatct tggagttgca agtactgttt ctcgtggaat
     9661 agctgatgtt ggtttgggaa atgaaaaagc tgctttacaa gtaaataata tcgactttat
     9721 tcctcttcac aaagagcgct atgatttagt tattaaaaaa gaagacttac aaaatccagt
     9781 ttaccaaact ataattaaca taataaactc tcctgagttt aaagctgaac ttcaaggtct
     9841 tggcggatat gatttaactg atactggaaa aactatagca aaagtttaag attaaaatcg
     9901 caatctattt tataagattt ataatttttc acaaaattta taacttaaac acgaaataaa
     9961 ttgcgatttt agtgtctcaa aaaatttatg tctttcattt tatcatccaa tacaaaatat
    10021 aatataatta attgtgcaat catattatat taatatatat ttacataata gtatttttct
    10081 ttaatgtaga ttaacatatg atatgtttta aacatcaaca cccttgcttt cttatatata
    10141 tgtggcaaca catatgagtt taataaatta tatacaatgt tgctttcttc ttgtattttt
    10201 tcaaccaaca agttatttat aaaattacct ttctcatttt caataatctc actccaattt
    10261 atcttaactt ttaatatata tcttcccact aggtatacac actctacaat aaataataaa
    10321 aatacaatac agtcagtacc tgttgtctta tataaattac ccgatactaa tggctttgcg
    10381 aagaaattat aaaaattcaa agtatcaccc gtataatcaa aaagtattaa tagcactcct
    10441 agaactctca atactttaat attcaacatg tttttatctt tattattttt gtttttcata
    10501 tatactacct ctcttttctt tagatttgat tgtaatctaa tactaactct tttatttgag
    10561 gtataaatga atttaattgg tagttatttt catttttact agaaattaat tcacattact
    10621 aaacaaaact tatatttaaa cataaaaata gcctatctat tttttaataa atacaaaaat
    10681 aaataagcta ctaatattca attaactttt atcacttaaa acattactat ctataaattt
    10741 aaatatttta ttgaagtata ctgtactatc atgttcaaat ccatcaacat gaccaatacc
    10801 ttctataagc cataggtctt ttttatcatg tttaactaaa tcatacattt tcttgctatt
    10861 ttgataatta caaacagtat cttttgttcc atgtataaac agtatgggta cattagattt
    10921 ctcaactgac tcatatggtt ttacatcact gtaccaaaat ccagatttaa acttattata
    10981 taaatctcca caaatcacca cataattgat taatatatct ggtattcgtt tttctagtac
    11041 tcccattcta attgcatctt tcatttcaga ataaggagaa tccagtatat aaaaatcgac
    11101 cttatcatct tttgaattta tctctgaatg catagcagct gtccctgctc ccatggaaaa
    11161 tccatgcact cctaatcttc cttcaggaaa tttatttttt acaaatttaa ctagactatc
    11221 taaatcatac ctttcataaa gaccaaatgt gtaattatct ccaccactat taccagtatt
    11281 tctttgatta taaactaata cattataacc tttatctaaa tatctatatg ctacctttaa
    11341 catttcatag taactacttc caattccatg aacaagtatc atagtatctc ttgtctttat
    11401 atttgaagtt ataaaaagac tttcaagctt gtaattatta gaactttgta tcattaattc
    11461 agatgtcttg tagtttttta atttctccag tggtttatct tttctctcag aaaatatttt
    11521 tactatatct tcgtttttta ttaactgctc taaaccaacc gaactattat acactaattt
    11581 gccaaatata taagatacaa ttaataggat taatagaaac acaattgata taattataaa
    11641 tttatttctt aaactaatac aatttttaaa actatttttc atatttattt accatacaat
    11701 tttcctatat tatattttct aagtaatagc ataatt
//
LOCUS       DACTGW010000117        11721 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.117, whole genome shotgun sequence.
ACCESSION   DACTGW010000117 DACTGW010000000
VERSION     DACTGW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11721
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.117"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            224..1549
                     /gene="cdeA"
                     /locus_tag="I9573_002348"
     CDS             224..1549
                     /gene="cdeA"
                     /locus_tag="I9573_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HAT4796962.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GISFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
     gene            complement(1644..2438)
                     /locus_tag="I9573_002349"
     CDS             complement(1644..2438)
                     /locus_tag="I9573_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4796963.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2439..3314)
                     /locus_tag="I9573_002350"
     CDS             complement(2439..3314)
                     /locus_tag="I9573_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796964.1"
                     /translation="MRSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3318..3686)
                     /locus_tag="I9573_002351"
     CDS             complement(3318..3686)
                     /locus_tag="I9573_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4796965.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3869..4528)
                     /gene="deoC"
                     /locus_tag="I9573_002352"
     CDS             complement(3869..4528)
                     /gene="deoC"
                     /locus_tag="I9573_002352"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAT4796966.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4564..6108)
                     /gene="murJ"
                     /locus_tag="I9573_002353"
     CDS             complement(4564..6108)
                     /gene="murJ"
                     /locus_tag="I9573_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAT4796967.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILITGIIFIGITSVMSAFLQIKENFIVVGF
                     GSIPYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKD
                     DSLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYSDRLNGFVTGTFTA
                     SIVSVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFER
                     GAFDARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVL
                     DLVLIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIM
                     GVLSYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKR
                     KLHLKK"
     gene            complement(6582..7466)
                     /locus_tag="I9573_002354"
     CDS             complement(6582..7466)
                     /locus_tag="I9573_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAT4796968.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(7757..8233)
                     /locus_tag="I9573_002355"
     CDS             complement(7757..8233)
                     /locus_tag="I9573_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796969.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGVLDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDKNCGCGCNHHHEN"
     gene            8500..9675
                     /locus_tag="I9573_002356"
     CDS             8500..9675
                     /locus_tag="I9573_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HAT4796970.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(9756..10559)
                     /gene="proC"
                     /locus_tag="I9573_002357"
     CDS             complement(9756..10559)
                     /gene="proC"
                     /locus_tag="I9573_002357"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAT4796971.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINNEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(10599..11183)
                     /locus_tag="I9573_002358"
     CDS             complement(10599..11183)
                     /locus_tag="I9573_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4796972.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         3919 a         1894 c         1298 g         4610 t
ORIGIN      
        1 atacatatat ttctaaaaat tttcttaaga aacagttatt ccttactaat gcaatatacc
       61 tatttttact aatataagtt tattaattag cattatacta atttatctta atatgatttt
      121 ttgttgattt taagctgttt atcgtataaa taaaattttt cttgactata tagttactat
      181 atagcttatt atagatagag agtaataaaa ggaggtaaaa tttatggaaa atttatttac
      241 aagaaaattc actacttttg aatttctaaa atttgtttct cctgcaatta tatccatgat
      301 atttatatct ttgtacacaa taatagatgg catctttgta tcaacattag ttggttccga
      361 tgctcttgct agtataaata ttgtactacc tataattaac cttgtttgtg gatttggaat
      421 aatgatggca acgggtggag gtgctatcgt ttctatacgt atgggtgaaa atagacagga
      481 tgaagccaac tctacatttt cttttatagt tttgttttca ttgattgttg ggatttcatt
      541 cacagtaatc tcatatttct ttatcaaaga aatatctatt ttgcttggtg caacagataa
      601 gttattacca tattgtataa cttatggtaa agttatgatt ttatgtacac cattttatat
      661 tttaaaattt atatttgagt actttgcaag aactgatgga aattctaaat ttagtttatt
      721 tctatcagtc attggtggtg taacaaatat aatattggat tatgtattta ttaaatattt
      781 tggaatgggt cttttaggag ctgcagttgc aactgctata ggtattattt taacttgtgt
      841 tttaggtatt atttacttct tatctaataa atctacactc aaattaagaa aaccaaaaac
      901 cgattttaga cttataagag atactatgat aaacggttct tctgaaatgg ttacagaatt
      961 atctacagga attacaacat tcttatttaa tgtagtagct ttaaaattag caggagaaaa
     1021 tggacttgct gctcttacta tagtattgta tgctcatttt ttaatgacat cagtctatct
     1081 aggattcgct gctggagtgt ctccattaat aagctataat tttggtgctg aaaacagtga
     1141 taaattaaaa gaaacattta aacattctct aaaatttata tttgtttctt ctcttttagt
     1201 gtttattatt gctttagtat ttgcaccatt tattgttagg gtttttgtaa gtccagataa
     1261 cacagtattt aaactagcct tacaaggatt aaaaatattt gcatttgctt ttttgtttgt
     1321 tggtataaat atatttgcat caggattttt tacagcattt cacaatggaa aaatttcagc
     1381 tattatatct tttagtcgtg cctttgtttt tataatcata ggaatcataa ttcttcctcc
     1441 tatgttaaac atgactggat tatggcttac agttccattt gctgaggtta taaccatatt
     1501 tatatctatt ctatttataa aaaaatataa aggtagatat aagtattaaa aattaatgct
     1561 taaaatcaac atttattaaa actaataaac ctgctttaaa atacaaattt acaaatttga
     1621 tttttaagca ggttttattt tagttataat aagtcatttg catactcttt aattggtgct
     1681 tttctaagta ataaaattat aaatattatg caaataacta taattcctac ctttgttcca
     1741 aataaaataa aaggattctg aaatgaataa ataatttttt ctaaatacaa tagcatttta
     1801 tgcatatttt tagttgcaaa attaactata taaattacca ttaatatcgg taatcctatc
     1861 aatatagtaa tactagtaac ttttctaact ctatatgtaa aagctccaag taatactcct
     1921 ataaaaccta tacatatttc atttatacat atttgtacaa aataatttaa ataagttagt
     1981 ggcatacttg atgttgaagg atctatcagt gctgatatag atgtccaaat catatctctc
     2041 tttagtaaaa ctgatagttc tatactgtaa cctgttataa tttctataaa taacttcgat
     2101 aaaaaaatca aaactatacc taaaatagaa atcacaaagc ttaaaataat taaatctatg
     2161 acaatagctt ttatacaact ttttcgatct gctcttatac ttaaagctcc tgaaaagtct
     2221 ttatttgcta tatttactcc atacacaaat agcagtatag ttatatatgt taaaatagga
     2281 ttcacaatcg tggataaatt agttttagat gcttctccaa aaatcttgag gataaacact
     2341 ggcaaaatat tcataacaat tcccacaatc ataaatataa ttatatacaa tttagtattt
     2401 ttattaaaaa attttatata tggctttaat tcattcaatt acaattcctc cttcttagtc
     2461 atgttaacaa acatatcttg tagccctatg tatcctacat caatatttga atttttaagt
     2521 attttttcat cattttcact aaaatcacca tagtaaaaat aagcaactgc atttccaaat
     2581 gcctttttag gttttatatt ttttatacat tctaattttt ctaactcttc tttatttcca
     2641 gtaatataat gagctttttg ttttatgaca tcaatttctt catctataat aatttttccc
     2701 tcatttaata ttattacatg ctccagtaaa tcatctattt catcaatcaa atgagttgat
     2761 attattattg tccttggatt ttttatataa ctatctaaaa ttatgttata aaactcttgt
     2821 ctatttactg catccaaacc aattgttggc tcatcaaata tggtgatagc tgaattagaa
     2881 caaataccta ttatatttga aagtatagtt ttcatacctc ttgacagttg tttatacttt
     2941 tttttagtat ttaaatcaaa atatttgcat aattcttctt ctaagccttt atcataactt
     3001 ttataaaaac tagaagaaaa ttcaaatatc tgacctactt taaaatctgg actataaaat
     3061 tctttttccc tgacgataca aacttcttcc aaaactttta tatcttcttt tgggttcttt
     3121 cctaatattt ctatctcacc ttcattttga attaactgat ttaccattat atttaataat
     3181 gttgtttttc cagctccatt cttacctaat aaaccatata tcttattttt ttcaaagttt
     3241 agagacatat tattaaaaat attttgcttc ttaaaactgt gagataaatt ttctatctta
     3301 atagaactcc tcatatatta atcctccttg aaattaatta taatatcaac caattcttct
     3361 ctatttattt ctaatttgtt agcttcttga agtaggtttt ttacaaaatt atgtttaaaa
     3421 ttttcttttc tagcttcttt tataatagtt tttgcaccat ctgatacaaa catccctatc
     3481 cccctcttct tatataaaat atttttatca accaatatat ttataccttt taatactgta
     3541 gctggattaa ttttataaag ctttgaaagc tctgttgttg atggaacctg ttcttcttct
     3601 tttattccat ttgatagtat ctcatcttca attgctcgtg ctatctgaat aaatataggt
     3661 tcttcattat ttaatactaa tttcataggt tcctcctttc tccattagtt agttactcaa
     3721 ctaattaact atataactta taatatcttg aaaaatattt tctgtcaata ctaaataaaa
     3781 aacagaggga aaattccctc tgttttttgt tctttatatt tataattaaa ttttgatttt
     3841 tgtaatccta tcccataagt taaaaacttt aataatctgt tgtatcttca ttttttaata
     3901 ttttacatat tgagctagtt cctaaccttt ctgctcctgc ctctataaat ttttctgcat
     3961 cagaaattga tttaactccc cctgcagctt ttatttttac attttttcca acatgctctt
     4021 tcataagctt tatgtcttct aaagtagctc cacctgttcc cattccagta gatgttttta
     4081 taaaatctga acctgacatt gttactattt cacacatttt tattttttca tcttcatcta
     4141 aataacatgt ctctataatg acttttaata ctttatctcc tatagctttt tttatttctt
     4201 ttatttcttt tcctatatta tcataatctt tattttttat atctgaaatg tttacaacca
     4261 tatcaacttc atctgctcca ttttcaatag catcttttgc ttcaaatact tttgtagcag
     4321 tagtttgata tcccaaaggg aaccctatca ctgtacatat ctttatttta ccttttagat
     4381 attctgatgc tcttttcaca tatgatggtg gtatgcaaac tgatgctaca ctcatatcta
     4441 cagcttcatc acataaaatt ttaatatctt cccatgtagt tgtagctttt aaaatagtat
     4501 ggtctactgt ttttaatata tgtttcataa aatcctccta aatttataat taatataatt
     4561 agattacttt tttaaatgca attttctctt tgcaatattc agtatcatat caacctcttc
     4621 aactttaaaa atagtcatta gtagtgtata aattccacct ccaactataa ctgaaatagc
     4681 tagtgataca aactcattaa atgttcctac tcccaaaatt ccaaatataa atttatacgt
     4741 aaagtatgat aaaacaccca taattaaaga tgcaactacc gatttcaaag ttgcttttat
     4801 tattttatct tgtccaaaat atccaacttt tctctttagg ttcaagaaga gtaacaatat
     4861 acatgcaatt gatgatgaac tagttgcaag agcaagacca ccatgagcca ttggttttat
     4921 taaaactaag tccaatacta tatttacccc tacagatata atcccattta ccataggtgt
     4981 ttttgtgtct tgtaatgaat aaaaaacctt tcccaaaata tctcttaatc caaatgcagt
     5041 cataccaact gcataaaaaa ttaaggcagt tgctgtcatt tgggttgccc tcgcatcaaa
     5101 agcacctctt tcaaaaatga ttctaacaac aggagttgca aaaaaaatag aagcaactga
     5161 aataggaatc atagaaatta ttatcatatt tatactagat ttaactgaac tagtaaactt
     5221 cttttgatta ttttctgcac ttaacttaga taacatagga tacataacag atactattga
     5281 agctgtaaat gtccctgtaa caaaaccatt taatctatct gaataagtaa gtgctggaat
     5341 gcttccttta actaatgttg aggctaaagt tgtatctacc aatgagttca cttgattaac
     5401 agcaacacct ataaatactg gtgaaagtaa tgctagtaat ttaataaggc tatcatcttt
     5461 aaagtttaag tagtataagt atttgtagtt cgttttttta acaaaaaaca tgtaaaagag
     5521 cagttgaact accatagcaa ccacagcacc aattggaagt atgtatggtc caaatacggt
     5581 acttaacatt attgaaataa ttattactat attatagggt atactcccaa atccaactac
     5641 aataaagttc tctttaattt gcagaaatgc tgacataaca ctcgtaatac ctatgaatat
     5701 tattcctgtt atcaatattt tagtaaattt tacggtaagt aaaaacctct ctccttcaaa
     5761 tcctatagca aatatactta caagttgctt tgagaaaata actcctaata tagctacaac
     5821 tatgcatata ccaactatta tatttaaaac attgtttaaa aactttagtg cttgcttttc
     5881 accttgttta ctacttatat cttgatacat tggaataaaa gtagtaacta tagcagtacc
     5941 tattgctgca aatataatat ttggtatatt catagctgtt aaataactct ctgtatataa
     6001 gcctgtacca taaattgatg ataatactaa ttctctaccc attccaagaa tcttagagat
     6061 tatagtaact atcatcaaat aaaaagttgc tttagctacc ttgctcattt tatcactcca
     6121 cttctaacat acctcttttt aatcaagtaa aatttggctc attagatata attctatcaa
     6181 ctaatttgcc ttgcttagcc ctatcaaaat ataattaaca cctatttctt aatatatcac
     6241 aaactacatt attcttcata tttttttatt tctccaataa gttttacatc tttcctatat
     6301 aaattgtata gcttcaatta tattcaaaga aatggagttg ctacccgata actttaaaag
     6361 taaaaaacag attttattat ctttttaaac tatattctaa catattttgt ccatattgag
     6421 aaattttact aaactattat gtactttatt aaataaataa aataacagca cacctataaa
     6481 atacaagtgt gctgttcata aacagatttt ataaaaaaat aattcctaag tagagattaa
     6541 catgctttct catactctca tatataatgt gtctaaaaat attaataaaa atgctttaat
     6601 tgttttaact tacttggatg tcttaatttt cttattgctt tagcttctat ctgtcttatt
     6661 cgttctctgg taactccaaa tacctttcca atctgttcta gtgttttagg gtcatcatca
     6721 tcaattccaa atctcattct taaaacttgc tgttcttgct caccaagacc tgtaagtagt
     6781 tcttctatct tttcttttaa attatgctgt tcaacttcgt gtattacaac atctttaaaa
     6841 tcagcatctg atggtataaa ttcaactaaa ctactatcct catcttcttt taatggagta
     6901 tccaaagata caacattttt atctcttctt aataattcca acactttgtc aacttctaag
     6961 ccacatgcat ctgcaatctc atctatagtt ggctctctta ataatacatt taaaagttct
     7021 tgtttctttt tctttacaaa atttattctc tgatgaagat gtataggtat cctaatagta
     7081 ttttcgcaat cgtcgatata tcttgttata ctttgtttta tccaccaagt tgcataagtg
     7141 ctaaatttgt atcctttttt ataatcgtac ttttctacag cttttataag tcctatattt
     7201 cctgcttgaa ttaaatccaa catatcaata ctatctcttt tatatctctt tgctatgctc
     7261 actaccaacc tataatttga atttataaat ttttcttttg ctactgaaga tgagttgatt
     7321 atttccttag ccagctctac ctcttctcct gctgacaaca ttttgtattc ctctatttcc
     7381 tttaaataca ttttcatcgc atttgataca tttgatgaac ccacacaaat ataattttca
     7441 taattatttt cttttttagt atccataatc aaaccccttc tattcataga atttgctaaa
     7501 acaaaaataa ataatttaaa cctacataat ctacaattac ataatatccc attctttaaa
     7561 tgaattgttt tgtatattat gtatacaaat ttataaaaac tatacctata atattattct
     7621 acttctaacc gcaatatcct ccttatatat ataatttatt ttattatatg ttgttatatt
     7681 cttagttttt tgaatgatat atgtctaaaa aaagactgtt tactaaacag tcttttttta
     7741 tatatatgct caatatttaa ttttcatgat gatgattaca tccacatcca caatttttat
     7801 catggtgatg atgttcttct aaatcttttg cctgttgttc catttcatga aactctttta
     7861 ccattatatc ataagttgga agagctgcct cagtatcgta ttcttcccct ctccaatcag
     7921 cgtacatcat agcatcccca ttagttaaaa ccaatctacc tgacatagtt atattatcca
     7981 cttcttctaa aaattctatt tgtttttgta aaacttctcc ttctaactct acatcttcat
     8041 ctaaacattg cactatcatt acaggtgcat aataatcttc ttcatcttta acatttacaa
     8101 caatatctaa aactccttct gcaccttctt caaattctgc caactcaaca tttgtatcaa
     8161 tcatatcttg agggactaat aaatcttcta ttaaatattt tattactcta tcttttacta
     8221 attcttttga catttatttt tctccttccg attctataac attatcataa tcatttctat
     8281 attatagtat actacattaa ttgttttttc aatctcttac aaaaattatt tttttattat
     8341 cttccttata ttcccttttt tattacaaag ttgtaatcgc attgatttaa aaaatagttt
     8401 atatttagta taatagaaat gtagcacata tttttattca atatattaat aaaaaaatag
     8461 attgaaaatg taatgtatgt taaatctagg aggaatatta tgaagtttaa aaaacaaatt
     8521 agttctattg ttgtgtctac tatgcttgta cttgggagta tgaacctttc ttatgcagat
     8581 ggtacaaatg tagttacact tggagctaac ttatcagagt ctcaaaagca gcaaatgctt
     8641 aattactttg gagtaaaaaa agaccaagtc ttagttttag atgtaacaaa tgcagaagaa
     8701 agacagtatt tacaaggtgt tgcaacagag ggtcaacttg gaaaagttac tatctcttgc
     8761 tcatacgttg aacctactaa aaaaggaaat ggaattaatg ttaaaacagc aaacttaact
     8821 tgggtaacta gttcaatgat tgcttcaacc cttacaacag ctggtcttga agatgcaaat
     8881 gttattgctg ctgctccata tcctgtaagt ggtactggag cattgacagg aatcatgaaa
     8941 gcatttgagg atgcttcagg aaagaaactt gatgaaacta agaaagaaat tgcttctgaa
     9001 gaattggtag ttacaggtga cttaggagat gatatagggc aagaaaaagc aacaggtgta
     9061 atgaatgata taaaaacaga aattgtaaag aatggtacaa aagataccaa tcaaatagca
     9121 aatacaataa ataatgttgt aaataattac aatattacta ttacaccaga acaaaaagaa
     9181 caaattaaag gtgtaatgaa aaaaatagct gaccaaaatt atgattacaa tagcatgaaa
     9241 aatacactag ataatgtatc agataatgta aatgaacaat taaagaaatt aggtgaaagt
     9301 gttaagggtt ctggtatact agatactatt ggtggatggt ttagtggaat aggagactgg
     9361 ttctctggtt tattctcagg cggagataaa aaagatttgg gaatcttaaa taacactaat
     9421 gatgagtctc ttggctctga tgctgtaata aattctacag aaggtgttca aataaatcca
     9481 caaaataata ctaattctaa caatgatagt gatacaaata gctcaaaaaa tgatacttca
     9541 agtaataatg aaaataacga tagttctcaa acaaatgaaa acaacacaac aaataattca
     9601 gaaaatcaat caaataataa tgactcagtt ggtttctttg aaaaaataaa aaattggctt
     9661 tcaggcttat tttagaaatt aaacaaataa aaagaattgc ctaaaatgga aattttctat
     9721 aaaaggcaat tctctttatt tacaataaat ttttattact cactcatttt tttagatttt
     9781 tctatacatg cacatattgc ttttatcaca gaagctctaa aaccttcttc ttcaagtact
     9841 ttaactgctt ctatagtagt tccaccaggt gaacatacca tatcttttaa ttcaccagga
     9901 tgttttcctg tctcaagtac catttttgca gaacccataa cagcctgtga tgcaaactta
     9961 tatgcttgtg gtcttggcat tcctgcaatt actgctgcat ctgctatagc ttctataaat
    10021 aaaaatacat atgctggtgc agaaccactt gcaccaatta cagcttctat aagatactct
    10081 ggtactacct cagtatttcc aaaagaatta aatacttctg taactgcttc taagtcttca
    10141 ttgtttatat ttttatttat agaaagagaa gacatggctt catttacaag agctggagta
    10201 tttggcatag ttctaactat ttttttatct tctcctataa ttgactctat atcctttata
    10261 gattttcctg ctgctattgt aactataatt ttattgtcat ctataaagtc ttttatctct
    10321 tccaaaatat catcatatat atctggttta acagctacaa ttactatatc agaggttcta
    10381 gttacttcct tcgagtcttt tgtagtattt attccaaaat ttgcatctat tctatcgaga
    10441 gttccttgac tatatgctga tgctataacc atattaggtt caactaattt tgaatttact
    10501 attcccccta tcatagcgct tgccatattt cctgcaccta tgaatccaat ttttttcact
    10561 ttagtacctc cttattgact aatagtaatc gttaaaattt aagcttcttt aactgcatca
    10621 gacttttcaa atggatttgg tatacttact accaaaattt tcactacctt attagtatag
    10681 ttgacccaat tatgattgtt cttttcacta ttatagtgta ttgtgtctcc tggatacatt
    10741 tctatttgtt catctcccaa aaacactgta agagtaccct ctaatacata cacaaattcc
    10801 tcaccttcat gtacgtaaca acaaatttcc tcactactct tacttggaag aatttcaatt
    10861 aatcttggta gcattgtttt ttcctttaaa ctagatgata aatgataatg tataaatgtt
    10921 gagttttcta catcaaatac ttctttttca taacttctta atacagctct tttatgctct
    10981 tttggcttca taaaaaagta agttaattct acatctagta cacttgctat ttttcctaag
    11041 gaatctgttg ccacacttgt taatcctctt tcaagttgtg ataaaaaacc aattgacaag
    11101 tttgtttttt cactcatatc cttcagcgtc atttgctttt gagttctgag ttgctttatt
    11161 tttgctccta tgtccttatt caaaaccatc acccctaaat aattttaatt ttttcataat
    11221 ataaatacaa aaatataaat ataggtactg aacctatctt tatttcattt aaaaaaaatt
    11281 tttataagta ttactataat ttatcaatct ttttttgtct acatttttac gattatttac
    11341 gatttatttt tcacatatgt atatttaatc ttgccaacta aagatacatt ttttataagc
    11401 atttactctt tatataataa aacttaagtt gttcatgtag tcttcttctg gtttaattat
    11461 taaaatttca aattatatta ttatttctac acattattac atcctttagt acaattataa
    11521 cataatttta caatttaatg atatctattc aatgtctttt tttactctac aacatatttt
    11581 attttagagt ttaatactat atatattttt tatatacagc cttgtcattt tttagattgt
    11641 caagagttat tgaagtattt tatgtagatt tatattttat tttctaattt ctcatttaac
    11701 aagaattgct tatgataaaa a
//
LOCUS       DACTGW010000118        11564 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.118, whole genome shotgun sequence.
ACCESSION   DACTGW010000118 DACTGW010000000
VERSION     DACTGW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11564
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.118"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(126..812)
                     /locus_tag="I9573_002359"
     CDS             complement(126..812)
                     /locus_tag="I9573_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAT4796973.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(861..1394)
                     /locus_tag="I9573_002360"
     CDS             complement(861..1394)
                     /locus_tag="I9573_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAT4796974.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDA"
     gene            complement(1438..2070)
                     /locus_tag="I9573_002361"
     CDS             complement(1438..2070)
                     /locus_tag="I9573_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HAT4796975.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYNTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2160..3239)
                     /locus_tag="I9573_002362"
     CDS             complement(2160..3239)
                     /locus_tag="I9573_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796976.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSANPLRFIKNI"
     gene            complement(3372..4718)
                     /gene="pepV"
                     /locus_tag="I9573_002363"
     CDS             complement(3372..4718)
                     /gene="pepV"
                     /locus_tag="I9573_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAT4796977.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4787..6067)
                     /locus_tag="I9573_002364"
     CDS             complement(4787..6067)
                     /locus_tag="I9573_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796978.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6194..6454)
                     /gene="hfq"
                     /locus_tag="I9573_002365"
     CDS             complement(6194..6454)
                     /gene="hfq"
                     /locus_tag="I9573_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAT4796979.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(6568..7518)
                     /gene="miaA"
                     /locus_tag="I9573_002366"
     CDS             complement(6568..7518)
                     /gene="miaA"
                     /locus_tag="I9573_002366"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAT4796980.1"
                     /translation="MERNVMKKIPLIILTGPTAVGKTDLSIKLAKDIDAEIISADSMQ
                     IYEYMDIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKGKCVL
                     VTGGTGLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRI
                     HKNNTKRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDI
                     MIKNGLVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYA
                     KRQITWFKRYKTARWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(7541..9508)
                     /gene="mutL"
                     /locus_tag="I9573_002367"
     CDS             complement(7541..9508)
                     /gene="mutL"
                     /locus_tag="I9573_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAT4796981.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKDKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(9519..>11564)
                     /gene="mutS"
                     /locus_tag="I9573_002368"
     CDS             complement(9519..>11564)
                     /gene="mutS"
                     /locus_tag="I9573_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAT4796982.1"
                     /translation="LDMFTRTNLELTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYV
                     EEPLINKSKIENRLDVIEEIKDDFILREDLNDILKNIYDIERICGKIAFERVTPKELI
                     HLKNSIEKLPNLKDTINLSNAKILKEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNI
                     IKSDFSDELKELREISKNGAFLVKEIENREREKTGVKSLKIGFNKVFGYYIEITKANF
                     KQAKLDETYIRKQTLSNAERYITPELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKN
                     IDRIQKVAKTIANIDVFVSLATVAHINNYVKPAINENNKLDIRNGRHPVVENIVGEEN
                     FVPNDTYLNRGENIINIITGPNMSGKSTYMRQTAIIALMAHIGSFVPAESADIPILDR
                     IFTRVGASDDLSQGQSTFMVEMNEVSLILKNATERSLVILDEIGRGTSTYDGISLAWS
                     IVEYIQKNIRCKTLFATHYHELTDLEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGAD
                     KSYGIYVAKLAKLPDEVIERAKYILKDLEKNHVYNSVAINGDKENNNIINSNLDEELV
                     KVNQSNFKNKYESLKIEHELIVKDYKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKI
                     NQEDSVKEVALTQISFDSVNRDILSEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
BASE COUNT         3540 a         2026 c         1185 g         4813 t
ORIGIN      
        1 ttaaacttat ttatactata attattaatt tgatgataaa agctattttc cttctaaata
       61 aaaaatttgt ctaaagtttc taaaaaattt agacaaatct taggttatta ttctttaaat
      121 tatttttaaa ttattccaaa tacaccaaat aatattggta taaatatagc tggtaacaaa
      181 ttagctactt ttatattcgt taatttaagt acatttagac caagtcctat aattaataaa
      241 cttccagctg ctgtcatttc tgtgactaca ggatcactta aaaatccatt taaaaatgat
      301 gctcccacac atataatacc ttcatacaaa aatacagata ttgcagaaaa cattacacct
      361 atacctaaag aagcagtaaa tataattgaa gctattccat ctataacaga ttttacaaat
      421 aaagtatcat gacttcctgt aagaccactt tctaatgaac caactactgc catggctcct
      481 atacagaata ataaactaga tgatataaaa ccctctgcta tagaatcttt tttatttcct
      541 ttagaaaatt tactttgtaa aaaagcaccc aacttattta cccatttgtc aatatctatt
      601 aattctccta tcagagcacc tacagctacg gatattattg tcactaatgt gttttttcct
      661 tctagtgctc ctgatatacc aatgtacaat acacataagg caattccatt cattatagta
      721 tcacttactc tttgaggtaa tcctcctttt attataagac ctactataca tccacctgca
      781 attgctagac tatttgctat tactccaaac attttttctc ccctcgatta gctaattgaa
      841 atttatccta ttattaaaac ctaagcatct atactttgta ttggatattt taaatattcc
      901 aaaaattctt caaaaccttt tctagatata tttacagttt tagtatttat gttaggatga
      961 aaatttaagt aatcttcttt aattacatct ttatctatgt aaagctcaac ttttctttct
     1021 gtatcattta ttaatgaaaa aggatttaca cttccaggaa gaagtttaag tacattgtat
     1081 agtctttcag gtgaagcaaa cgataatcta gaacttccta tcttatcttt aagtgagttt
     1141 aaatcaactt gtttttctcc tttaactaca ataagataat acttatttcc tttggcattt
     1201 cttaaaaata aatttttaca ttgacacccc tttgttattc catctaaaat gactaattgc
     1261 tctgctgtat atacagcttc atgctctact acatcatatt caacatttaa tttatccaaa
     1321 atatcatata ttttagtttc attcatgcta gattcatttt catcttgttc ttttttatta
     1381 tttaaatcac tcatactttt ctccttaaac acttcatcat aatatcattg tgaggtatta
     1441 ttctactata gctattgctc ttactggagc tccatcagca tcattatact taaatggtgc
     1501 tgctataaat agaaattttt ctggaacatt ttctaaattt gtaagatttt ctatgattat
     1561 ttttcctttt tcaaataaaa tatgatgcac ttcaaatact gatgtgttat atctatccac
     1621 tgatagcata tctattccta tgccttttat attagtatta gctatatagt ttgcagcttc
     1681 ttttgttaat gtaggataac ctacatagta ctgcttttta tcccaaaatt ttgaccaacc
     1741 tgatttaaat attataaaat cactattttt tatttcatct tcatattttt ttagatattc
     1801 taattctata ttctcatttc ccttttcaag ttccaataca taagctttcc caacaaaatt
     1861 ttctatatcc aacttatcta gggtctcacc tttagtatac atatgtcttg gtgaatccat
     1921 atgagttcca ttgtgagaaa atacactaat taatgtttct tgataaccat tttcttcaat
     1981 aatagctact ttcttgatat ctggtctatt aggctctgca tatacaggca tgtcattatg
     2041 tgtcacatga gttaaatcaa aaaccttcat ctttatatac cccctatgtt tttattttct
     2101 attatatacc taaattcatt ttatttctat cagattttat tccattatat accatactac
     2161 tatatatttt ttataaacct caagggattt gccgaaaaat cttggaaaga attatcagta
     2221 aatataacat cttcacccaa aaaacgttta taccttcctt cttctttctt taaagtaatt
     2281 ctctgttttt gtgaatgatt attaaaaacc attatcttaa gaggaggttt ttttctatta
     2341 gtctgcactc tttttaacat gtattttatg tgcatatcag cttcaggaaa tgaatatcca
     2401 caaaatatta aaaggtcagt atctcttaaa gttttttctg tttcattcca aacattactt
     2461 aaaaatatgt ttgacatatt cttaaaatat gttggtggca ctataatagg ttctgttaac
     2521 tcaccacact ctaaacattt tgtctctgaa ctattttcaa taagcttcat gacaccacct
     2581 tcatgtggtg taagagttac actattacaa atggggcaat aaagccaatt caatgaccca
     2641 tgtatcttat ataatttgat taatggttct tttggttttc tccaatttaa ctcctcttta
     2701 aaatttgtaa actccacacc ataatcaagc ataattggca aatcattctt atctcttaat
     2761 ccagtaactg tattgtctat atgtatatca taatttgcac taataaaagt agtatcaaga
     2821 agcaaatcat tttccaaaag attttcaact aaaagtttat gatatttatt atctgttcta
     2881 ggtgtattat gaattgcttc agccattagc agtattaaat attgtcttaa aaaacgtata
     2941 ctatcacttc tgttatttag attttctagt ccaaaatttc taaatgcttc ttttctttgt
     3001 tctgctaagt ctaaaagacc taaaacttct tcaaaggtag gaaaatttgc attatctata
     3061 ttatctttta ctatatcaat attgaacatc tgtttaaaga aaatatacaa ttctctattc
     3121 attttggtat tataatttgt gggatttagt tttttaaaat attcgctaaa aagttcattt
     3181 tgaataggta gattctctgc tgctgaagct cctgctccta aaaatattgc tattttcata
     3241 tagttcctcc taatataaat attactccta tttttatatc cattaaatat ccatatcatt
     3301 tattttatga tacaacaata aagctgtctt ttactagaaa aaatcctatt acaagacagc
     3361 cttatattat attaaatctt taataattcg taaatagaaa gagcatatat ttgagtagct
     3421 tttataatat cctctactat taagtattca ttagcttggt gttctgtttc tggttttcct
     3481 ggaaaaatag ctccaaatgc aacacaatta tctaaagctc ttgcgtatgt tgctcctcca
     3541 gatgaaagtg gtgttccatc aagaccagtt tcttcttcat atacttttct aagtgtctta
     3601 acaagaagag tatcttctgg aacatagatt gaatccaaga aatcatattc ttcataattt
     3661 aaattgtatt tatctgtcat tttctttaac tcttttacaa aatcatcctt tttgtatgtt
     3721 actggaatcc ttacatctat tcctgcaaat tctttttcgt tatcaatagt tactctacct
     3781 atatttactg ttagtttacc tgatacatca tccttgcaat taggaattat attgtttcca
     3841 ttagcatctt caccaataac ttctgctaaa aacttaatta tattagaatc tatacctata
     3901 ttattaagag ctatacaaag tctagccact gcatttatac cagtatcact tgctgctgaa
     3961 tggacacttt ttccaattat acagatttta ttaccttcaa cagtatactc aaaatttaat
     4021 ttatcaagtt ctttttttaa tttatcagag tattttccaa tatagattgc tttacctgga
     4081 actgcattta aagcctttcc aactgacaac tctatatcac ttttgctatc acaagtcaaa
     4141 tgaacttgta aaagaccttt ctctgcattt gttattggga atctagaatc tggagtaaat
     4201 ccatagtttg gtatttcttc attattttct ttatatttat ttatacatct ccaaagattt
     4261 tcttcatcag ccccaaaaat gaatcttatc ctcttattaa agtctacatt taaatccata
     4321 agagctttca ctgcataaat tgctgatatt gtaggtcctt tatcatcttg tgttcctctt
     4381 ccatacaatt taccatcttc taaaacagct tcaaatggag ggtaattcca gctttctaaa
     4441 tcaccttctg gaaccacatc aacgtgacca agtataccta tcattttttc accttgtcct
     4501 atttctgcat atccataata tccatctttg tatactgtct taaatcctaa agatttacat
     4561 aagtctaata tacctcttaa agctttatct acattttcac caaatgggca attttcagtt
     4621 gcttcagata taacactagg tatttttaca ctttcttgta ttgaagaaat catttcatct
     4681 tgtagagaat ttactttttc tttaatttgc tctttcatat tatcaccctt caattttcat
     4741 cataatatac taataaatat tttgaccttt ttaaaaaaaa atatgcttat tttttcagtt
     4801 tttttataaa ttcaatcgct tttaaagtcc ccatttttga atggtcaaat gtaagtccac
     4861 cttgaaaata aacattataa ggaggtctta ttggagcatc tgcacttaat tctatagaag
     4921 aaccttgtat aaatgctcct gctgccatta tgacttcact ttcgtatcca ggcatgtccc
     4981 aaggaacagg ttttacataa gaatcgactg gtgcagcttc ttgtatacct tcacaaaaac
     5041 ttattacttc atcagcatta ttaagtctaa tacattgaat aatatcactt cttaagtcat
     5101 catattttgg taatacatca tatcctaatt tttcaaaagc tcttgagcaa aagatagctc
     5161 ccattacagc ctgagatact atataaggcg ccaagaaaaa accctgaagt acatttctag
     5221 ttgttccaaa agtaagacca cattcttttc ctattccagg agaagtcatt ctataagata
     5281 taagctcaat caaatctttt ctaccagcta tatatccacc tgtcaatgca agcccaccac
     5341 caggattttt tattaaagag ccagccataa catctgctcc aacatcagta ggctctttgg
     5401 tatctaaaaa ctcaccataa caattatcaa ccattactat agcttctggt ttaacagact
     5461 ttattacttc tatagcttct tttatatcac taattgaaag tgattttctc caagaatatc
     5521 cttttgacct ttgtatcata acgagtttag ttctatcatt tattttgttt ttaattcctt
     5581 ctaaatctaa gtttccatcc tctaaaaaat ctacatcatc atatgtaaca ccatactctt
     5641 tcaatgaacc tttttcttct cttatgccta taactccctc taaagtatca tatggtctac
     5701 cagtaacaga taaaatttca tctcctggtc taactatacc ttgtatacat aaagtaagtg
     5761 catgtgtacc atttacaatt attggtctta caagagcatc ttctgtatta aatactttag
     5821 aataaatttc ctctattttt tcacgaccaa tatcattata tccatatcct gtagtccagt
     5881 taaagtgcat atcacttagt ttagattctt gcatagcttt taaaacttta tattgattat
     5941 attctcttat ttcttttatt tcttcaaact tatcttttat ttcttccatt atttcttgcg
     6001 atagtttaaa agtatcatca tctattccat agtagtcttt taataattct tttgtctcat
     6061 taagcatttt atcacctgtc tctttattat tttatttaaa catgtaaagg agactatttt
     6121 actaaaacaa ataaattata gtttataagt tagtataagt ctcctctcaa tcatcttaaa
     6181 ttaaattaat tatctatctg ttgttattat tattgttgtt ttggttttga tttgttagat
     6241 taatatactt tccaggttgt atagttgata cagcatgttt ataaatcatg ttttgttgtc
     6301 tattatcccc ttctattaat attatataac tatcaaaccc ctttacaagc cctttaactt
     6361 gcactccatt aactaaatat atagtaacag gtatcctttc tttccttgca ttatttaaaa
     6421 acaaatcttg taaatttaaa actgtatttt tcattagatt tgtaccctcc caagattaaa
     6481 ttgaacttct aaaatattcc taccttatat tctatataag ttatgacaaa ttttcaatat
     6541 taagttgtat acttaaaaat ttatatatta ttttatagaa tctttaatat ataagattat
     6601 ttcatttttt aattcgtcta tattttcata ttggtctaaa tcaaaccatc ttgctgtttt
     6661 atatctttta aaccatgtta tctgacgttt tgcatacctt ctagaatctc ttttaattat
     6721 ttcaatagct tcttcgtatg tatattctcc atccagatac ttcaatatct ctttataacc
     6781 tataccttgc attgatatca tatctttttt aaatcccata cttaaaagtt tttttacctc
     6841 ttcaaccaat ccatttttaa tcataatatc aactctcata tttattcttt gatacaaatg
     6901 ttctctatct ctatttagaa ctattattat tggttgatat tcttcattaa attttaaatc
     6961 acttgaaaaa tcattcattt tcttaccact tagacaaact tcaagagctc ttatgaccct
     7021 tttagtattg tttttatgga ttctattagc agattcttca tcaagttctt ttaacttatt
     7081 atgcatgtaa tctattccct tttcagcaag ctgtttttga agttcttctc ttaattcatt
     7141 attggcatca gattgtgcaa aatccatatt atatattaaa gagtttaaat aaagtcctgt
     7201 gcctccagtt actaaaacac attttccctt ttcatttatt tcatgtatat atttttttgc
     7261 tctttgttga aactctgaaa ctgaaaatgg atagtcaggc ttaacttcat ctataaggta
     7321 atgcttcact ccttgcattt cttcctctgt aacttttgca cttccaatat ccatatattc
     7381 atatatttgc atagaatcag ctgaaattat ctctgcatct atatcttttg ctaattttat
     7441 tgacaaatct gtttttccaa cagcagttgg acctgtaagt attataagtg gtattttttt
     7501 cataacattc ctttccatat attttaaata tccaaatatt ttacataatt cttttaaaca
     7561 ttttttctat ttctgtctta gatatttcga ccattatagg tcttccatga gggcatgtaa
     7621 atggattctc acacttttct agctgttcca ataaactttt tatttcaata tcgtatattt
     7681 tatcatttgc ttttatagca gacctacatg ccatagatgc aaatctctct ccttttaaat
     7741 cataattact tgtaatttct tctatattat caattatttg aagtataaat ttctcagttt
     7801 caggtactcc aaatatagta ggaacacatc ttaccattat atgattattg ccaaaaattt
     7861 ctagttcaaa accaaatttc ataaataact ccaagttatt ttcaatttga agcatatcta
     7921 catttgatac ttcaataacc actgggtcta aaagtatttg catattgata tcttgtctat
     7981 agaacttctc catatatctt tcatatagta ttctttcatg tgcagcatgt tggtccaaca
     8041 aatacataga atcgtctttg ctcaaaatta tataagtatt aaatacaact ccaattacag
     8101 aatatccata taaggaaaac ttgcgttttg aactacacaa gtattcttct tcactgtcct
     8161 taatactatc tcctaagtaa taatttcctt catttttaat tccatcaact tgaaactctt
     8221 cttgtatatt atcatttgca ctatcttcac ttagataatt aatgtttttt acatcatctt
     8281 ctacacttgc atttaggact tcacttactg attgaaattc atttattggt ttttcacttg
     8341 ataaagtcac aacttcaatg acttcatcct tatctttagt aatattacta tttttaggag
     8401 tagattctaa taaatcatta tttcttaatt tataatctgt acttttttct ctactgttaa
     8461 tagcgattct aggttgtgtt tttttatcac tataagtagc atattttcca ataagatttg
     8521 agtgtattaa tttaaccttt aaaaaatctc taagttctat atacacttct tgttcttttt
     8581 caaatttaat ttcaagttta ttggggtgta tatttacatc tatacatgat gggtctactt
     8641 ctatatttaa gaagcacact gcatgtttac ctattggaat tatagactta taactttctg
     8701 ttattgcatc tatgattatt ttacttttta caaatctttt atttatatag atatgttgta
     8761 agtttttatt tgaacggtat atattgttat tacctatata tccattcatt ttaaagtgat
     8821 tgcacttaaa ctctacatct attatatttt ccgttatttc ttttccatat atacttctaa
     8881 tagtgtttac aagttttcca tcccctggag tatttaacat ctgtttattg ttatttgtat
     8941 atttaaattg aatgtttgga ttacctattg caagtttatt tatcaaatca cttatgttta
     9001 tagtttctgc atgagtagat tttaaaaatt tctgtcttgc tggtgtatta aagaaaatat
     9061 cttttataat gatggtagtt ccattagtag aacctattgg ttcttttgat attatttttc
     9121 cgccttcaac atatatttta gttcctatta tttcatcttt agttttagtc gtcatttcta
     9181 attttgatac agctgaaata gatgccaatg cttctcctct aaaaccaaga gagtataaat
     9241 catacaaatc atcaattttt ttaattttac tagtagcatg tctcaaaaat gatttttcaa
     9301 cttcacttga aggtattcct attccattat ctgtaatctt aattagactt tttcctccat
     9361 caattatatc tatggaaatt ttatttgcac ctgcatcaat ggagttttca ataagttctt
     9421 ttactacaga agaaggtctt tctacaactt cacctgctgc tatcttattt atagtcaaat
     9481 catctaggat gttaattata tttttcattt ttacacctct aagattttat ttcttttgct
     9541 tttctttgaa gattataaag agagtttata gcatctaatg gagtcatatt taaaatatcc
     9601 agatttaaaa tttcttctga tagtatatct ctatttacac tatcaaaaga gatttgagtt
     9661 aaggctactt cttttacaga atcttcttga ttaattttct ctttatcatc attttgcttc
     9721 attaaagcaa cttcatcatt taatgcttta aatttagaat ttaatttatc ataatccttt
     9781 tttatatgtt tgtagtcttt tactataagt tcatgttcta tttttagaga ttcatattta
     9841 ttcttaaaat tactttgatt taccttaact aattcttcgt ccaaattact attaattata
     9901 ttattgtttt ctttatctcc atttattgca acactgttgt aaacatgatt tttttccaag
     9961 tctttaagta tatattttgc tctttctatg acttcgtctg gtaatttagc taatttagca
    10021 acatatattc cataactctt gtctgctccc tgaggaatta tttttcttaa aaatattatt
    10081 ccttccccat cttctttgac tgctattgaa taatttttaa cctctttaaa ctcttcttct
    10141 aaatcagtaa gttcatgata atgtgttgca aataatgtct tacatctaat atttttttga
    10201 atatattcca ctattgacca tgctaaactt atcccatcat aggtactagt tccacgacct
    10261 atttcatcta atataactaa acttctctct gttgcatttt ttaatataag ggatacttca
    10321 ttcatctcta ccataaaagt agattgacct tgagataaat cgtcactagc tccaactctt
    10381 gtaaatattc tatctaatat tggaatatca gcagactctg ctggtacaaa tgagcctata
    10441 tgtgccataa gagctattat agcagtttgt ctcatatatg ttgatttacc tgacatatta
    10501 ggaccagtta ttatgttaat aatgttttct ccacgattta aataagtatc atttggaacg
    10561 aaattctcct ctcctactat attttcgaca acaggatgtc taccatttct tatatcaagt
    10621 ttattatttt catttattgc tggttttaca tagttattta tatgtgcaac tgttgcaagg
    10681 gacacaaaaa catctatatt agctatagtc ttagccactt tttgtattct atctatgttt
    10741 ttatatattg tatctcttat ctctacaaat atctcgtatt ctaaagattt aatcttttcc
    10801 tctgcatgta gtatcttttc ttctatttcc tttaattctg gagttatata tctttctgca
    10861 ttactaagtg tttgctttct tatatatgtc tcatctagct ttgcctgttt aaagttggct
    10921 tttgttattt ctatataata tccaaaaact ttattaaatc ctatttttaa tgatttaact
    10981 cctgtttttt ctctttccct gttttctatt tcctttacaa gaaaggctcc attcttagaa
    11041 atctctctta gttccttaag ttcatcacta aaatctgatt taattatgtt tccatctttt
    11101 atggttatcg taggttcttc tagaatagct tcatctatta aattgtatat atcatctaat
    11161 ttatccattt ctgaaacata ctcttttagg atttttgcat ttgataaatt tatagtatct
    11221 tttaaattag gtaacttttc tatcgaattt tttaagtgaa ttaactcttt tggtgttact
    11281 ctctcaaatg ctatctttcc acatattctt tctatatcgt atatattttt aagtatatca
    11341 tttaggtctt ctcttaatat aaagtcatct tttatttctt ctattacatc taatctattt
    11401 tcaattttag atttatttat caaaggttct tctacatatt ttcttaaaag tctacctccc
    11461 attgctgtag aagttttatc aagtacatgt aataatgaac cttttttctt attccctctt
    11521 atagtttgag taagttctaa attagttcta gtaaacatat ctaa
//
LOCUS       DACTGW010000119        11498 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.119, whole genome shotgun sequence.
ACCESSION   DACTGW010000119 DACTGW010000000
VERSION     DACTGW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.119"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(72..497)
                     /locus_tag="I9573_002369"
                     /pseudo
     CDS             complement(72..497)
                     /locus_tag="I9573_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(970..1257)
                     /locus_tag="I9573_002370"
     CDS             complement(970..1257)
                     /locus_tag="I9573_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796983.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(1277..1552)
                     /locus_tag="I9573_002371"
     CDS             complement(1277..1552)
                     /locus_tag="I9573_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796984.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAIGMLKGVDYEPLIVATQVV
                     AGTNFKFICNATSVTNPPHDYLAEIIVFKPLPCDEHNATITSINIIK"
     gene            1740..2559
                     /locus_tag="I9573_002372"
                     /pseudo
     CDS             1740..2559
                     /locus_tag="I9573_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(2818..5385)
                     /locus_tag="I9573_002373"
     CDS             complement(2818..5385)
                     /locus_tag="I9573_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HAT4796985.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTNQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMDGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIALQSEKE
                     LWGLSEWVKQNNSINLYLQNSPAIKIAEDFMSSVMPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5388..5993)
                     /locus_tag="I9573_002374"
     CDS             complement(5388..5993)
                     /locus_tag="I9573_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT4796986.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6367..7827)
                     /locus_tag="I9573_002375"
     CDS             complement(6367..7827)
                     /locus_tag="I9573_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HAT4796987.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDRAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8158..8736
                     /locus_tag="I9573_002376"
     CDS             8158..8736
                     /locus_tag="I9573_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT4796988.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIKRHNLKLKIEKEKAFGVCNS
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9028..10278
                     /locus_tag="I9573_002377"
     CDS             9028..10278
                     /locus_tag="I9573_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4796989.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10439..11008
                     /locus_tag="I9573_002378"
     CDS             10439..11008
                     /locus_tag="I9573_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT4796990.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVDFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAVAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3802 a         1994 c         1588 g         4114 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaattcac gagtgtgcgt
       61 agctattttt taatcaatac aattactgca catatagatg cagaattttt aaatacttca
      121 cctaatgaaa ctgctgcact aatatcatgg gctactcccc tattgtcgct aaaacctgca
      181 gtatcacata attttgcatc cattccttct attggttttc tcatatttac aatgtttggg
      241 tataaagttt gagaaacagc tcctgaacca atctctgcca cagggtctaa gcaatcaata
      301 tatgatatac cagtatcttc atcttctact tccatcatta cacatcctgc aagatagtta
      361 attgcagtac ttttgccaca tcctgtccca ccaagaatta gaattaaatt ttgattttca
      421 gccttattta ttgctacttt tccttcattt aataagagtg ttatttcttc taaaagttga
      481 ttgttatttt ctggcattta ttttccccct tggttatata ttttatttaa ataaagttta
      541 atcccttaag tagattacaa agtttttaga ctatatttaa atttgttttt tatttttaat
      601 aaatttgcat tttcactcaa taattattct tctttgttta ttaaaataac tgtgttgtat
      661 aatataattt ttaccaacac agttatttta tttaatgcgc ttattttatt gtattttatg
      721 taatacagtt gtttttttat attatatgtc cactattttc ctatggttat tacatataga
      781 tttttaatta tatttgtacc attaatttaa acattcctat actactgctt aagttttaca
      841 cctataccag cttgaatatt ttatttttat ttgcttaaat agttcaacta tactggttta
      901 ataggtttcc tttagtttaa atagttcacc tatacctact tgaatagttc acttttattt
      961 gcttaaattc tataccaata ctggcttaaa cattatacta ccatgattgt attgtctatc
     1021 ttcaactaca ataaaaacag gttctaaatc tttagtagat gttattttta attctaaaaa
     1081 aacattatca gttggtaata tttttacatc ataattattt aaattattat tcgtgatact
     1141 attaaataca tcaccttgac gttcaaaaaa aacattatca actattacaa aataaacatc
     1201 ttcttttcta ctattttcaa cctctatagt attttgttta gttacattta gtctcatctt
     1261 ccacctccta ttaatattac ttaattatat taatacttgt aattgttgca ttatgttcat
     1321 cacaaggaag aggtttaaaa acaattatct ctgctaaata atcatgtgga ggatttgtaa
     1381 ctgaagtagc attgcaaata aatttaaaat ttgttcctgc tacaacttga gtagcaacaa
     1441 taagtggttc ataatcaacc ccttttagca ttcctatagc ttcattaaat acttttaaat
     1501 cttcttcttc gacagcccta aattttgacc atccaccaac aaaaagactc atataaaaac
     1561 ctcctttata tatatattta tttatatgat agtaccaatt ttcttaatat tcaatatatt
     1621 acgtctaact ggtcaataaa aatgcataac tggataaaaa gacttataaa atagcttaac
     1681 tttacatata ataagattat tctaaaatat gagacatatc ttgctttgaa tatattctat
     1741 acaacaattt tccaggcctc attgccaagt ttaaggtaac tgactttgga ttccaacttc
     1801 ttgatgctaa taaaaaatat tttgatatgt tcaaagataa tgtaaatttt ctattagatg
     1861 atataaattt agaaggttat cttaacaatg tgtataaata tcataatgat tttaaatctg
     1921 ggagatgcgt ttgtttttct atttctccac aaactattaa tggagatatt tttcacatgc
     1981 aagttgtagc tgaatgtata gattgggaag atgataagcc tatatatctt ttaatatatt
     2041 cagatgtaac gacattggta aaacaatatg tctcattatc atttttcatc caagaatttt
     2101 atcaagaaat atgataaaat agctaaaaaa tatgatattc cttctcacct tatagaaatt
     2161 gagattacag aaacaattgt atttgaagat gtcaaagtat ttagtgaaat aataaatgaa
     2221 attcataaaa atgactttaa atgctctatt gatgattttg gaagtggcta ttcttcaatc
     2281 aatatgctta agggtattta tgttgataca ttaaaattag atagagcatt ttttcaatca
     2341 gaagatttag attctcaacg tgaaagaaat ataattgtat ctataatcga actagctaaa
     2401 aagttaaaaa tgaccactgt agctgagaga atagagacca gttttcaaaa agatttcttg
     2461 caagaaataa actgtgatat gttacaagga tacgtgttct ctagaccttt gcaaataaaa
     2521 gactttgaaa aacttatgtt tcaaaactcc tccaattaac aatataaaat tgcttattat
     2581 aagtgtatta ttgtatcaac aaataaagga attgtctcaa aattgatttt ttctaatcaa
     2641 atttttattt tgagacattc tcattttttt gcataagaaa atacccacca agctacggtt
     2701 tttcttaccg ctgttcggtg ggtattagta gtattatgaa atctgaaaac tttaagtatt
     2761 actacttccg ctaacgcaat cacaaaggtc gaaagcttca aagcgaaaat aggcttttta
     2821 ctcatcaaac gtcaccgtac ctgccaaccc gtccaccgtt atcatttgtc cgctcttaat
     2881 agttcgtgtg gcaatgtgtg tagccataac ggctgggatg ccatattcac gtgcgacaat
     2941 gcttgaatgg ctaagaggac caccaatgtc cgttacaatc gcacttgcag atgaaaataa
     3001 tggtgtccat gctggagttg tggtaactgc aaccaaaaca cttccaggtt gaaagctttc
     3061 aaaatctgct ggactattca gtacacaagc tggagccgtc acaacaccag tactggtacc
     3121 aatgcccgtc aacacaattt tcccatcttt ctgcttctgt ggtgcttgtg atatagtttt
     3181 tttgtttttc tctggcaact tggacggtga cacataaccc atgtattttt taagttcagc
     3241 ttttcgaaca ggtattatcc cgttcatatc agatagagac ttgtttttat ccaactgctc
     3301 tataagtacc tccaattccg attttgtgag ccaataaata tcatctaaat gtgaaatagc
     3361 tcctcctcgt atgaatcgtt ctgagatttc ctgaaacata caacgaatca gaggatgtcc
     3421 cattcccatc aaataaatag cattttcacg cataggagag gtttcctgtg cccaatggag
     3481 caacttaaaa aatagttttt tacgcaatcc acctatgtgt tgtaaaatct cttcttctgc
     3541 atgttttcta cgcttttcaa acgtggtctg gcgtaaaaaa ggactttccc cttttccttc
     3601 cacaaatgtt tttacggatt caaaagttgg tgtaagtgtt tcctgcggtg tggaatacgc
     3661 aaaatcaaac tcataagcgg tacgaccaaa ctctttaaag tacttattga ttcgattttt
     3721 ccattctatc catacttcct gtgaaactcc aacaggcata actgaagaca tgaaatcttc
     3781 tgctatcttt attgcaggac tgttttgcag ataaaggttg atgctgttat tttgctttac
     3841 ccattctgaa agaccccaca actccttttc cgattgcaga gcaatcgtat caaacccaag
     3901 caggaaaacg gaagtatccg ttataccagc acgtcgaact gctccttgaa ataattttgt
     3961 aaataatgtt tcactgatag aagcagcagg caatgtaagc tgaatccttg tgaaataaat
     4021 acatgcggca taaaatattg tctgaatacc atccataatt tgatgggcgt tcatcgtatg
     4081 caggggcttt tcttcccatt gtttaatagc atcctcatat ccttttcgtg ccgtttccca
     4141 gcgtgcaacg ctgttcataa gtgtcctacg taaagagcgt ggggaaaggg atttgatagc
     4201 aatcaaaagt ggctttgatt tagcagaaag ataaacatat ccattaataa ccgtatatgc
     4261 gccgtttttt ggtaataatt ttttcgcact tttaccaaac atatctatcc ataaaagctc
     4321 cgatgcacgg tttacgattt caagaccaag cgtggcgaac aaaggtgtaa caggatttgg
     4381 caagtgttct gccaaacttc cctttgtgta tacaacatcc tgttctggca atacccattc
     4441 cggtggcaga acagtaattg gacgagcttg aacaatgtac aatttatctt tttccagtgc
     4501 ccactctaca tccataggca tttgataata cttctcaatt ttctttccaa gctgtatcag
     4561 ttgcattacc tgattacgag taagagcata tttttttctc aacctttcag gaaccatggt
     4621 ttcttctgtt ccttctgaag tacgaaccgt cataatctct ttatttgcca cttcatacga
     4681 tataattcgt tcggagtcct tatccactac gattgtatcg ggtgtaacta agctagaaac
     4741 caccgcttca ccaagccccc atgcagcgtt aataatcatt tcgctacgtc taccattgat
     4801 tgggtttaac gtaaacataa taccagatga atcggaaaat gcaagctttt gtactacaac
     4861 agcaagtgcg acaatctcgt gctttatatt attcttcaca cgataagcaa tagcacgagc
     4921 tgtccataag gaagcccagc atctctttac agcgtcaata actttatctt caccttgtat
     4981 gttaagataa gtttcctgct gacctgcaaa agaagcgtca ggtaaatctt cagcagtcgc
     5041 agacgaacga acagccaccg atatgttccc taatccggca taagctgttt taattgcgtc
     5101 cgacacttcc tgtggcattt ctccattatg gaaaagctcc cctattttct tagatacatc
     5161 ttcaagttgg ttggtattat tggaatcaat accatctagc agtttattaa ttcgagactg
     5221 aatatggttc gtctcaacaa aaatctggta tgaagcagtc gtaacatgaa aaccgtctgg
     5281 cacagggatg cctgctgtca atagttttga taaagacatc ccttttccac caacaatttc
     5341 aatggttgct tgtttatgtt ccagtggcaa gacataataa atcataatca ttcacctcca
     5401 tcattcataa ttccgtgcag aaatatattc attgtttcat ccaacatttt ttccgaacta
     5461 tccgatggtc gtgacgtgta gctgattgat aacatggagt taattaacaa acgtgtcaca
     5521 agagcggtat ccgttttgcg aaagatgcct gtggtcacac cattatcaat taccgatttt
     5581 ataatatctt gatatgcata acgagcatct ttaagtctct ttcgatactc ttcttctaaa
     5641 taatcagatt ctgtatttaa ccacatcagt acagttctat actgctctgg aaatctaaga
     5701 tgattaagca tgatttttct aaggcactgt tgtggtgaag gttcaccatc aataatccta
     5761 tgaacttgtt ggatagtttt ttctatttct ttctcaactg catatacaac aatctcgtct
     5821 ttcgttttga aaaaatcgta tagacttgac tttcccatgt tggctaaaac tgcaatttct
     5881 ctcattgatg ttttttggaa tccattttca gccatgagac tcaaagcaac agcaactatt
     5941 ttttctcggc gttctgcttg ttgttcaggt gtaagcacaa ttccttttgg catagggcga
     6001 tacctccttt tagcgaccaa tggtcgtttc ttttttattt atcatacagc gaccatcggt
     6061 cacttgtcaa ggttaatttt tttcattgta tatggtactc gagataaaaa tgttcatttt
     6121 agaaattgct ctatttaagt agaaataggc ttcatcatta gaaaacaaga ctataaattt
     6181 gagatattta tcatgttcaa atttcatcaa tatcaaagtt taagatatta ataaaaaaag
     6241 aattactaag aaataacata taaagattca aaaaaggagt tgaacttaaa attcaattac
     6301 aaaaaatcaa atttaagaca actcctttat tctttatctc tactattctt tatctctatt
     6361 tatttattac aactccctaa catttaccac tactgcctta ggtctagtca tagcctcgat
     6421 tgaatactta acaccttgag ttcccatacc agatgccttt acacctaaaa atgggaagtg
     6481 gtctggacct ctttcagtct tattatttat ttgaacagta ccaacttcaa gtttatcagc
     6541 tatataaaat gccctatcaa tatcacttgt aaatactgat gattgaagcc catactctga
     6601 ttgattagca atttcaattg cttgatttat atctttaact cttataattg gaagtactgg
     6661 accaaatggt tcttcccaag caattttcat atcaacattt acattatcta acaatgttgg
     6721 atagaataaa ttattttttc taacattacc tgttattaac tttgctcctt tatgtaatgc
     6781 atcatcaacc aacccttgaa caaaatcagt tgctttatta tctataagag gagtgataac
     6841 tacttcatcc attggatttc caattgtcaa cttctctacc aatggcttta tttttccgac
     6901 taatttatcc gccacactct cttgtactaa aattctcttt actgccgtac atctttgtcc
     6961 tgaatatgaa tacgctccag atactatatt cttagctgca aaatctaagt ctgcatcttc
     7021 taatactata gccgcatctt ttccaccaag ctccaataat agtggtacca ttccagatat
     7081 tttagatata tgctgaccaa cttcagtact accagtaaag ttaataaaat taattccttt
     7141 atgcgttaca acataatccc ctatctcact acccctacct gtaatagtat taagtactcc
     7201 atctggtatc ccagcttttc tcatgatttc taccacatga agtgcgctaa tagccccttg
     7261 tgtagcaggt tttaatacaa ctgcatttcc accaataagc gctggagcaa tctttgacat
     7321 tgataaattt acaggataat taaaaggaga tatagctaaa acagtaccta atggaaccct
     7381 tgaaacataa gacatcttat ttcttgttcc accagggaaa ttatctccac taatggcttc
     7441 accctccata ttttttccca catcagctgt gtatcttaaa aaatctgctg ttctctcaac
     7501 ttctgccctt gcagatttta tgtctttggc tatttcaagt accagtatat tagctatctc
     7561 atctatattt tctaacaata aatcagcagc cttatgaaat atattggctc tcttgtatac
     7621 aggcatttca gcccatactt ttatactctc ttttgtgttt tttataacct catctacctc
     7681 atgttttgtc atagattgta ctttcccaac aagagaatta tctattggtg agtaaatttc
     7741 tattggcttt ctcgaattag attctaccca ttttccattt attaaatttt tatatacatt
     7801 ttcttttgtt ttaagttcat taaacatatt tttattcctc tctctgatat aactttgtgt
     7861 atttaaatta tagccattta ttcgttatgt aaacatttct ttttttcata aacgtttacc
     7921 tataaaaaaa attttaagtt aacgtttttc tagattcttc tattttaata acatactcgc
     7981 tccaaatcta tataggtcac actttaatat caatatacca atataccaat ataccaatat
     8041 aaatattata aacataaatc acttgacaaa tttataagta gacaatatta tatatctatg
     8101 acttaaaata tttttaaagt catagatagc atatgcagat agaaaggatt gataaaaatg
     8161 gctaaaagtt ttacagaaat agaaaaagac aatataagaa aaagactaat ttcagaatgt
     8221 gaaatcaact ggtctaaata tggatacaaa aaaactaaca ttgatgaact ttgttcaaaa
     8281 tcaggaatcg caaagggttc tttctataca ttttttgatt caaaagaaaa gttatttttt
     8341 gaagtactaa atggaatcca aaacagactt atatcatcag tagatgaaat tttaagtaac
     8401 atgcctccaa aagaaggctt tgcaaaatcc ttaaaaatgt tatatcgatt atatgataaa
     8461 aatccatttt tatattcacc aaataatcct gatttcatca gcctaataaa taaactacct
     8521 caagatatgt ttttacaact tgagtccaat aacatacaaa gtttttacga cttaattaag
     8581 agacacaatc tcaagttgaa gatagaaaaa gaaaaagcct ttggtgtttg taattctcta
     8641 ctatcaagtc tattggtaaa agaaaatatt gaatataact atattgaagt atttgatttt
     8701 atggtagata atcttgtaga acacatactt gaataacaat ttaaaataat ttttctaaat
     8761 atttctttat actaaagagg attaatatga ttgaattaat gaaaaaaata aacaatatca
     8821 tttacaaaat aaatgataaa ctaaatatac acacaaaact tatagacccc agagttaact
     8881 cattccactc tactctaagc catctataat agttcgtata tttctaataa tattagatat
     8941 taaagtaaga aaaaaattcc ttataataat tgctattata tcaattatta taaatatttt
     9001 aataatctta gataggtagg tatatatatg gtaaaatcct caaacacttt taataaaaac
     9061 ttcaacttga tgatattagg tcaaataatc tcattgtttg gtgcatcaat acttaaattt
     9121 gctttatctt tatacatact tgatataact ggtaaagcag aaatatttgc tactatacta
     9181 gcagtatctt caatcccaat tatagtattc tcccctattg gtggagctat tgcggatata
     9241 ttcaacagaa gaaacttaat ggttattttt gatttttcaa gcagtcttac agtactaatc
     9301 ttagcagtat tcttattcaa caataatggt tcaatattac ttgtaggaat tattatgacc
     9361 atattatcag ttataagcac aatgtatcaa ccaactgttc agtcaagtac acccctttta
     9421 gttgataatg agcatttaat gaatgcaaat ggtattgtag ctggagttgc atctcttaca
     9481 aatattgcag gacctgtatt aggaggtgtc ttgtatggtg tgattggaat taatgcaatt
     9541 atagttataa gttgtgtaag tttctttcta tctgcaatta tggagatatt tatacagatt
     9601 ccttttatca aacaagagca aaacagcaac attataaaaa ctatatttac tgatataaag
     9661 gatggtattg ttcatatatg taaagaaaat agatttatac taaaaatact ttttttagct
     9721 atagcaataa acttattttt aagctctatg ttaatagttg gtatccccta tatagtaaaa
     9781 atcactatgg gagctagtaa cttcttatat ggtctagcag aaggagcaat ttcttttagt
     9841 tcactggtag ctgctgttag tattggtatt ttctcaaaac atctaaaaat cactaatttg
     9901 cacatttcct ttattatatg tgcagttatg ttcataccaa tggcggtttc agtatatcca
     9961 tttatattaa acacaggatt tccagttcca tttttaatct ttatagtatc atctatgcta
    10021 atatttttta caacgactct tatctcaata tatgttataa cagtcacaca attaaaaact
    10081 ccaaatgaac tcttaggaaa agtaatgtca attctttttg caggaacagc aattgcaatg
    10141 cctcttggtc aggttatgta tggtaaactt tttgatttat tatcagatag agtgtacatc
    10201 attatactag gagttggatt tattacacta cttatagcat ttgtatcaaa gataacattg
    10261 aggaacgaaa atctttagat acaaagaatt tagatataaa gatttaattt tcactaattt
    10321 ctgaatttaa atgcaaaatt gcgtatcagt atgttgacgt tttagaaatt gattgatata
    10381 atcaatggaa aataaacttt ataaaaattc aaaatattca gctagaggag tgataaaaat
    10441 gagttacatg ccaactttag aacaagcatg ggaaatatta agaaaataca ataaggaaga
    10501 atttcatata agacatgctc aaattgtatc tggagttatg agatactatg caaaggagta
    10561 cgaccctgag agagtagact tttgggaaat agttggactt ttacatgatt tagattttga
    10621 aatgtatcct gatgaacatt gtgttaaaca aagagaatta atgacagagc ttgatttaga
    10681 taaatcaata atcgattcta caataagtca tggatatgga ttaactggtt cagatgttaa
    10741 accagagcaa tttatggaga aagtattatt tgctgtggat gaacttactg gtttaattgg
    10801 tgctgttgca ataatgcgtc cttcaaagag cgtttctgat ttagaattaa aatctgttaa
    10861 gaagaaattt aaagataaat catttgctgc tggctgttct cgagacgtta taagaagtgg
    10921 tgcagaaatg cttggatggg aacttgataa gcttatccaa acaactatag aagctatgag
    10981 aagcttgatt ccagagatgg caatatagct cctcatacat ataccataga agttacatct
    11041 ttaaagagca tttctctttt tgatgcaact tctttttata ttttgtagaa taaaaagttt
    11101 aatattttaa actcactata taatctataa taaatactat aaataatata atgtataata
    11161 gtcataaact ttctaacctt aaaagtttct aatactcaat aaacaattct aatatacaag
    11221 tggcattata tgatttaatt atatatcttc aatttatata ttaaaataaa tatatatatt
    11281 aaattcttat acaccaaatt tttacattaa acttctatgc accacattta tacatattaa
    11341 attcttatat attaaatttc tatgcatcac ttttatacat attaaattcc tatatattaa
    11401 acttttatgc gtcactttta tacatattaa attcctatat attaaacttt tatgcatcac
    11461 ttttatacat attaaattct tatatattaa acttttat
//
LOCUS       DACTGW010000120        11464 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.120, whole genome shotgun sequence.
ACCESSION   DACTGW010000120 DACTGW010000000
VERSION     DACTGW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.120"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(123..797)
                     /locus_tag="I9573_002379"
     CDS             complement(123..797)
                     /locus_tag="I9573_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4796991.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFMDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIDILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYII"
     gene            complement(949..2856)
                     /locus_tag="I9573_002380"
     CDS             complement(949..2856)
                     /locus_tag="I9573_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAT4796992.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSLIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEVKTKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMNDLSGIYILSLVICLIIFVIYGAVS
                     VNKFKSVVYKN"
     gene            complement(2857..3609)
                     /locus_tag="I9573_002381"
     CDS             complement(2857..3609)
                     /locus_tag="I9573_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4796993.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPAVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(3927..5081)
                     /locus_tag="I9573_002382"
     CDS             complement(3927..5081)
                     /locus_tag="I9573_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4796994.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMILSLFGCLAYGLIVLLNYLQE"
     gene            complement(5084..5551)
                     /locus_tag="I9573_002383"
     CDS             complement(5084..5551)
                     /locus_tag="I9573_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAT4796995.1"
                     /translation="MFFKRKKEASTINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(5565..6053)
                     /locus_tag="I9573_002384"
     CDS             complement(5565..6053)
                     /locus_tag="I9573_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4796996.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(6067..7398)
                     /locus_tag="I9573_002385"
     CDS             complement(6067..7398)
                     /locus_tag="I9573_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HAT4796997.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARILGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMIEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(7412..8977)
                     /locus_tag="I9573_002386"
     CDS             complement(7412..8977)
                     /locus_tag="I9573_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAT4796998.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFLFTFLIKKFDMKTPGREEDNEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKVVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(9146..9931)
                     /locus_tag="I9573_002387"
     CDS             complement(9146..9931)
                     /locus_tag="I9573_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT4796999.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(10098..11297)
                     /locus_tag="I9573_002388"
     CDS             complement(10098..11297)
                     /locus_tag="I9573_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HAT4797000.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
BASE COUNT         3951 a         1943 c         1127 g         4443 t
ORIGIN      
        1 tattaattct gaatatctat tttttaaaat atctatagca aataaataga tatttattat
       61 actaactaca actaaaggta gagcaatata cttagaccta ccttttaaat attttactag
      121 cattatatta tatatcctat ccccttctta gtttttataa aatcatcaaa acctagttct
      181 ttaactttac ttctcaatct attcatattt acagtaagtg cattgtctgt cacaaactca
      241 tcattatccc atagagtcat cataagactt tcccttgata caaccctatt cttatttttt
      301 aataataatg ttaaaatttt tatttcgttt ttagtcaact ctatactttt atccttatac
      361 ttatgtttta taatcccttt ccccacatca aacatcaaat cttcacaata caaaatacta
      421 ttagaatcac tataattata gactcttctt attataccat ttattttggt aaccaatatg
      481 tctatagaaa atggtttggt aatataatca tctgctccat tatttatacc cattattaaa
      541 tccatatcag aatctcttga tgataaaaat attaatggta catttgaaat agctcttatt
      601 ttttcgcacc agtaaaatcc attatataat ggtaaattta catctaaaag tactacatct
      661 ggttttttat ccataaattc atctaatact acctcaaaat catcaattaa ttctacttta
      721 aatccccatt tttcaagtgc taacgcaatc tcttttgata aatcaatatc gtcctctact
      781 aataaaattc tatacatttg tatattctcc attatcctaa tatttcacta tgccatgaat
      841 tattaatatc ataacatatt ttatataaat ttattaccca aattatttag ttaatcaaca
      901 aggagactct tattacaaga atctcctttc tctttattaa tatatcaact aatttttata
      961 cactacactc ttaaacttat taacagatac tgctccatat attacgaata ttatcaaaca
     1021 tatcactaaa cttagtatat atataccaga taaatcgttc atcaataaat ctcctgctaa
     1081 tctcaatgca aaataactag atatagttgc acttaacatt ggcaaagtat aaaatatagc
     1141 tacttcttta agtatagctc tatttatatc tttttctgta gtacctattt ttaataaaat
     1201 cttgtatttc tctttatctt catatgcatc actatatatt ttcatatata ttatacttcc
     1261 agctacaatt gcaaagacta agaataaaaa cacaagcact acatatgcaa acttcatcca
     1321 ttgactctca ccttgagctt tatatccatt ctgaatatat ccatttttta aacttaaatt
     1381 cttttcaata ttgttgaaca ttacttggct attttcttct tgaaatacct ttgcaccata
     1441 aaaatatact tgttgaccct tatctcttag tttaaaatat gtatcatcat ttaccactat
     1501 aatactttga ttaagtcctg tccctaatac tggaactttt acttctcccc ttgaaatatt
     1561 atataactga ccatcaagtt ttgtcttgac ttcttttgta gctattgatg ctatcgtttg
     1621 aggacttttt agctctatta cattatttcc actaactaat ctttcaccat actcttttaa
     1681 ttctttatca ttgccattca ctttaagagt attacaaaaa tcactatatt tcattatagt
     1741 atctgttgta tcataagctt cttttatatc tgttttcttt aagattgtta gttctggatt
     1801 tacggagtac tttatcttat tagtatcttt tattatgttc tctatttgtt ttttattcac
     1861 ttcatcttta tccattgaaa ctatgctaat agtgtacaca tttctttgtt cttgggcatt
     1921 cttatgtata tgcttcatag caactgaagc accaagagtt gatattgttg tagttataat
     1981 tatagctatt atagcataag ttttatagtt tttatttaat cggtagttta tattgtttat
     2041 cgcaattata ttatttccat taaataatat ttttttattt ttgattataa tatcaaatat
     2101 aacaggcata actgatttga ataagccata agtacctact attattatac ctataacagg
     2161 tagtacacta gttatatctc cagttcttat attcgtagaa agatagtatc catatcctat
     2221 tactataagt gatactatag ctaatatata tatacctatt cctacctttg gtattttttc
     2281 ttgtttttta gatatattga ttagatttat tacactactt ctacatattg ttataaaacc
     2341 ttttacagcc attattataa atatagaaat aaagattaaa gtagcatata gtatagcttc
     2401 tatgctaagt ccattagata cctttatatt aaatccagat aattttatta tcactacttg
     2461 aaaaaatcta gatgttatta tccctataat tattcctgtt atacaagaac ttattcctat
     2521 aagcatattt tctacaaaat atatttttcc tatagtatat aaatctaatc ccataaagga
     2581 caatattcca atttctttag acctattctt aaaaaatata tttgttgcat accatataaa
     2641 gaaaaataaa aatactgata gtatcattat tacagctact aacactgata gtgtaaattt
     2701 tttattttcc tcttgtaaca catttatagc atctccatat cttaataatt caaaattagt
     2761 cagcacaact actgaaaaaa ccatagatat aaaaaatgct agataagttc ttatgttact
     2821 cttaaaatta ttataagcaa ttccaagtat actcacttac atctcacctc ccatagaggc
     2881 tagcatatcc ataatttttc cataaaattc ttttctacta tttcctttgt ttaatttaca
     2941 ctttatattt ccatcattta tcatatatac ttcatttgaa taacttgccg ttagagggtc
     3001 atgtgtaatc attataatag tagcgttgtt ttctctgttt attttttcta atgattctaa
     3061 aagttcatat gctgatttag agtccaaagc acctgttggt tcatcagcaa atatgactgc
     3121 tggatttgta attaatgacc tagctgctgc gcctctttgt ttttgtcctc ctgataactg
     3181 atatggatat ttatctaaat gattttctaa tccaaatttt ttagctatag aaaatacctt
     3241 agcttctatc tccttgcttt tcttttttcc taatgctaga ggaagtgcta tattgtccat
     3301 tagtttcata ttgtctaata gattatagtc ttgaaatata aaccctattt tttctcttct
     3361 taattttgat agttcattat tttttacttt tgatatatta attccatcta gtattatttc
     3421 tcctgaagtt tgtttgtcaa gtgtagacat taaatttagc aatgttgtct ttcctgcacc
     3481 agatggaccc attattgcta taaaatcacc ttcattgact gtaagagata tattttttaa
     3541 cacatttgtt ttaaatcctt ttattccata atcttttgat atatttttta tttttaatat
     3601 ttctttcata ataatagcct ccagttgcta attttttaat atatatttat tttaaaggtt
     3661 ttatataaat aaatccttaa gtaaatatta cattttaact gttagcatta cacttttgta
     3721 acatatggaa gactattata attatagtgg aaatatctta aaataaaagc atgattcact
     3781 tttttgtata aacaaagaaa agaagcccct aagaagcctc taatctatac aataattctt
     3841 ctctataaaa aaccaaaaat acaaccttga ttgtggcaaa tacaggtact ataaacaagt
     3901 gatattacta ctatatatta aaacaattat tcttgaagat aattcaataa gactatcaag
     3961 ccataagcta agcatccaaa aagacttaat atcattaatg tataattata cccaatatta
     4021 atactcaaaa aaccagcaac agttggaata atcagagatg aacctcccat aaatatactt
     4081 aaagctatta attggctact acttctaagt tttttaggag aaacttcaaa tattaatctt
     4141 tttgaagtaa ttaaaattaa aggagtagaa aacacttgaa aaacacacag tataataatg
     4201 atatttgaat catttgttaa agcaaataat agaaactgaa ctgttatcat aatagcactt
     4261 aattttaata agtttaaact gctatatttt tttaataaat aagaaccagc cagatatgtt
     4321 ggtatctcta ataatgactg taaagaccac tttaaagata tctgagaatt tgtagcattt
     4381 aaagctatca ttttatctat tacagtacag ttatttgcta ccaccattga gtataataag
     4441 aataaaatac atactagtaa gatatatttt ttatctttta ataattcaat aatatcttta
     4501 ttagttacat ttattttctt attaatcttt tcaatatcag gtaataatag tatatttata
     4561 ataagtaata ctacaattaa accaatcgct acagataatc caaaatatga gaatctaaga
     4621 atcaagtaag atgctaccat acttccaata gcccatccaa tcgacccaaa agtcttaata
     4681 aatgacaaat aattattaat attatctcca caacctatta tccatgtatc atataaacta
     4741 cagcaagtat ttaataatcc tcctgataaa gcaacaagta ataagtatat tataaaagtt
     4801 gcagattcag tagaaaacat aataactgat gcaattccat aaactgataa actaattaaa
     4861 acaatttttt tcattgtttg atacttatca gctaatactc caaataataa ttgaaataat
     4921 atattggtta ttgcatagct cgataatata attcctcttt caaacgctga gtaacctaat
     4981 tttgataaga aaggtgtgaa ttttgttgaa gcaaaggcaa aaataatata tgagaaaaaa
     5041 tagaatatga taaatttaaa tttaatcgct ttattaatca taatcaatca aaattgataa
     5101 ctttaatacc actatttcct tcctgattta agtttgaaag atgatatttt accgtattag
     5161 gattctctac aataactaaa ggaatatata aagtattttc aagtaactta ttatcaattt
     5221 ctactaactt ttcccccata ttaacttctt gaccttgttt aacttctaat ttaaagtttt
     5281 ctttttttgt tgttgcacta tccaatccaa tatgaataag aatttgaaca tcattttgca
     5341 atttaattcc tatagcatgg ttagtttgag caataaaact tattactcct ttagtcggtg
     5401 atttaagaat attgtccttt gctatcatag caaatccatc tcccattacc ttagttgaaa
     5461 atactttatc aggaatttct ccaagattaa ttatttttcc atcaatgtat gaattaattg
     5521 tacttgcttc tttttttctt ttaaagaaca ttatagacac tcctctacat aattaacttt
     5581 attctaatta tcattacaaa taacgatatt aagcatactg caattgagga aaaaagagcc
     5641 ttatagctct ttccccattg tagttcttta atactgctta tgatttgttt aattccaaca
     5701 aatttataaa tataggtaaa catgataaca atagttattg tgtaaatttg gaatatctgc
     5761 cctaataaga ctaaatagaa agcaatcatg ttttctttta tctctttatt cttttttata
     5821 ttgtaaataa catatgcact gtatactgca ctaaagttag agattaaatt taaacttgca
     5881 ttaacatcat tgtttaaaag ttgtgaaaat gaaatgtttg taaataatga tgtcaattct
     5941 aaatacacta tatataaaac aggtaatatt agaaaacaaa ccataaccac tgaaagaaat
     6001 atattttcat cttttaatac tgctgttata ttttttattt tatttgttat catgtttttg
     6061 ttctccttat ttaaaagttg gccagaaatc cttattagcc acaatcattt catctaagat
     6121 ttcttttgct acttttgcac ttggaacagt tttactcata gtaaacgact gccataattt
     6181 ttgataactt ttttctatcc atgcttctac agctaatttt tcagaagcaa cttgttgttc
     6241 cattaaacct ttttggaaag ttggaatttt acctatagac ataggttcaa ccatatcttt
     6301 tccaacaagg caaggaattt caatcattgc atcatctgca aaattttcaa tagctccatt
     6361 attaggaaca atcagtaaaa atctttcctg agtattaaat gctaatgcac aagctaattc
     6421 aacaataaat tgagaatgtt cacttgcatc aaaccatgta tcccttgctg tgccattttt
     6481 tataattctt tcacattctt cgaatgtatc cttttctctt ctatccataa tttcatttgc
     6541 tcttgtataa tttggattag catgttcaac agtttcatca gccattaaat aatattttaa
     6601 ataagtatta ggtaatgtag tcggttctat ttctaataag tcctttgctg ctctcatagt
     6661 ttctaaccaa ctagattctt tgtgttgacc ttgaggtgta aattcctcat atcctttttt
     6721 agcacaatat tcctgtaatt taggcattaa atcatttcct tctttgtcct taatacttgt
     6781 ccaccacccg aaatgattca atccataata tctaacatca aagtctttac gagaatttaa
     6841 acctaaaatt ctagcaaata tttcctctaa acatactggc atatcacaaa tattaataat
     6901 tcttgaattt ggtcttaata cacgacatgc ttctgcaact atagctgctg gatttgaata
     6961 attaagcatc caagcatttg gagaatattt ttccatataa tcaatcattt cgattattgg
     7021 acctattgtt cttaatccat aggcaattcc acctgcccca caagtttctt gaccaacaac
     7081 cccatatttt aatggaattt tttcatcttg ttcacgtaaa gcatataaac caacacgaat
     7141 ttgtgccatt acaaaatcaa tatcagtaaa tgcttcttct ggatttgttg tataactaaa
     7201 tttaatttct ggatttttct ctctaacaat tattgcacat gcttcagcaa cttttctttg
     7261 tctctcatca ttattatcat ataatttaat tgattcaata ggaaatttag caacactttc
     7321 aattaacatt gttacaattc cagcagtata agtactaccc ccaccagcaa ttagtatcct
     7381 ctgtcttttt ctttccatct ttccttctcc tttataataa attttcaaat tcttctctta
     7441 cacttggtac tgaaagccca ataataatct gaatagcttt tccattatgc actaaaccat
     7501 gtgcacctac taccttaaaa tcttcttctt tagaaataat tgagtcatcc ttcaaggtta
     7561 atctcaacct cgtagcacag tttgtaacat ctacaacatt ttctgctcca ccaactaatt
     7621 ttatgtattc agatgcttgt tgagaggctt tactcaattt atttcctttt tcatctttag
     7681 tttctaagta ttcattttta gtatatagtt ttgtttcttc attatcttcc tctctaccag
     7741 gagttttcat atcaaacttc ttaattaaga aagtaaataa aaagaaccag ataaatgaaa
     7801 atataatccc tacaacaact tgaacaacat ataccttcca gtgatacatt ccaagaggaa
     7861 gccaatttaa tacaacccag ttaataattc ctccaccaaa atcaccaaca actcctaagg
     7921 caaacgaaat cgtttgcaag catgcatcta aaaatgcatg tactaggaat aaaagtggtg
     7981 ctacaaataa aaatgtaaat tctaacggct cagtaattcc agttaatact gcagtaagag
     8041 tagcaggaat cattaaacct aatacttttt tctttttttc tggctttgca gttacataga
     8101 aagcaccaaa tacacctaat gaaccaaaca ccttcgataa tccagataat gaaaatccga
     8161 ttggatacat ttcctttaga gtcttagctg aagtttgaaa gtcctgaata tgagtagccc
     8221 aatatactga tgtaccacct ggaacaacaa cactatcata acaaattggt gcatatacaa
     8281 aatgatgtaa tccagttggt attaaaatct tttgtaagaa tgcataacac caaactccta
     8341 tatttccact atttataaaa aacgtttgca aatgtctaat aacattttga atatgaggcc
     8401 aaataaaaca agttaataat gcaactggta tcataacaaa gaaacaaatt gcaactacaa
     8461 gtgatgagcc tctaaataat ccaataaaat ctggtagttc tttatcataa aatctattat
     8521 gaatataaat gacaatacct gatattaaca aagcaccaac cataccagta tctaatgttt
     8581 taataccagc aatgcttaca agtccacttc cagcacttat ttcagctgcg aaatcaactc
     8641 caaagctttt tccccaaaaa cttaacatag aacctacaaa ataattaaaa gtcaaatacg
     8701 ttgctaaagc ctccatacaa gctcttgctt gttgcttttt tgcaagtgct attggtaaag
     8761 aaatagcaaa taataatggt aattgattaa ataccatcca agctccacta gatattacac
     8821 cccaaaattg gtaccaaata ttttctggtg tagcaagtga acccataatt gactgatttt
     8881 gaaatacaat tgagaatcca accattatcc cggcaaaagc aaataatagt gttggtgtaa
     8941 acattgcccc tccaaaacgc tgtattttct tcatcatata aattactccc ctcctcatat
     9001 ttttagttta tctttggcct tagatatcta ttattttaac aggttaataa atatatacaa
     9061 tctttagggt acatttggag cttattcaca gtcaaataag tttttgcaca tttttatgtt
     9121 caaattctca taaaaaaaag aatgactaca atttgcaatc aatcccttct gtaatcattc
     9181 tatgcctttt ataaatacta tatttaataa ataataactc aatcaatata aaatataaca
     9241 ttgtcaattt ataactaggg tgcatttgta aaaatgagat attagtcgct gaaatataaa
     9301 ttgattcatc acttaaatca gtcaatgtat tatttttaaa ctctgtaatt gaaattattt
     9361 tagttcctat taattttaat cttctagcaa tattaagtgt attctcattc tcaccactta
     9421 gggatataat aatcataaca tcatttttac tgacaatatt gtaaaaagaa tcatcaaatg
     9481 ttcttccctc aaaatcatat attatttctt gacatgataa gaacattctc tttatctgtt
     9541 ttgcaaccac attttgaata tcacctgaac cataagcgta tattgttctt gattcataaa
     9601 gaagtttaca caatgcatca taatctttat ttttttgatc ttcaacatac ttaattattg
     9661 aattacatgc aatatcaaat gactgatttt ctatgacatg aaccttttct gattcccagc
     9721 ttaataatac tttaaactca ctgaaacctt ttaaatctaa ttttttaaca aatcttgtaa
     9781 tagttgcacg agacacatta catttcatag caagttcatt tattgacatc ttactacaat
     9841 gttttttatt tgcagttata tatttccata ttaataaatc tgtatcattc aatttattat
     9901 aatttaaaaa aatcatttct tctaatctca taaactatta tcccctttaa ttaaaaatcg
     9961 tcataaatat tataccaata ttcaacatta aattacagcg tattcttata tttactattt
    10021 agcccataaa attgactata aaaaatactg ctataattga tgtttaaaaa aatgtagttt
    10081 taattttatt cttttatcta ataattaata ttttcaagtt ctagtgcttt tcttatgcaa
    10141 ttatcatctt taaaatcagc agtcctttta caatctttta ctttaaattc tggaatagtt
    10201 ttaaattcct catcacatat tccattctta tttaaataca tacaacttgc cattctaact
    10261 attagaccac tattttttaa attaaacaat acagggtcta tacaccctcc atctccgcca
    10321 gtagtttcac caactaaagt cgcaaattta gtttctttac aaaacattgc aaatgactct
    10381 gatgatgaat atatttttct atcaacaagt aagtatatat tacccttaaa ttttgaatca
    10441 tctgtagatt ttatagtata ggaagtatct tcaaaatcat taaaatcttt tataatttct
    10501 ttaggtgcat tttcctttac acttgctggt aaattttcta caggatttaa ttttatattt
    10561 cttgcatttg tatatttttt tactatttca ttattatttc tataaattct ataaccattt
    10621 attttgacat cattttttat gagtttagat acgattccct gccaatacct atcacttcca
    10681 cctaaattac ctcttatatc aataattaaa gctttatgtt tttctaaagt gttaatataa
    10741 tctccaatta tttttaaatc attatttata gagccattct ttgatgacat tgatggtaaa
    10801 tacatatatc caatctgacc cttaataaca tcctttaaaa ttaattcttt ttttacaaaa
    10861 atatcatctg gcatttttat tttaagattc atagagtcat atctatccac aactttttta
    10921 tcattaagaa aatcatacca atcattatta gagtatgatt tcttaaaaag ttcatatctt
    10981 ttcttattat ctattacttc tgtgtgttta ttatttaaat cagataatat agatgacagt
    11041 tcttctataa attcttcatc attttcagtg ttttttattc gttttgtata ttcatcctta
    11101 tttgctaacc aatcaatatt attaagtctc ttatttacac ctagatatgg atatcctgtc
    11161 tttacaacat tatacacata attaaagtca tccacctttt cctcttcaga caactgattc
    11221 ttttgagaat aagtagaatt acctaataag attataaatg ctaaaataat tactaaaaat
    11281 aatactttct ttttcatgta attctcatcc actttcagat tttatatttt aatacattgt
    11341 atcattattt tattatcacc taaacagtta aaagaaattc ttgaagatat attccactat
    11401 tattctattt aaaaattttt atttgtattt tcaaaattat ttcatgaaag ttataaaata
    11461 acaa
//
LOCUS       DACTGW010000121        11462 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.121, whole genome shotgun sequence.
ACCESSION   DACTGW010000121 DACTGW010000000
VERSION     DACTGW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.121"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            374..919
                     /locus_tag="I9573_002389"
     CDS             374..919
                     /locus_tag="I9573_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIC"
                     /protein_id="HAT4797001.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            932..1945
                     /locus_tag="I9573_002390"
     CDS             932..1945
                     /locus_tag="I9573_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAT4797002.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            2167..4140
                     /locus_tag="I9573_002391"
     CDS             2167..4140
                     /locus_tag="I9573_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT4797003.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKENNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            4209..4568
                     /locus_tag="I9573_002392"
     CDS             4209..4568
                     /locus_tag="I9573_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797004.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4603..4959
                     /locus_tag="I9573_002393"
     CDS             4603..4959
                     /locus_tag="I9573_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAT4797005.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4996..6288
                     /locus_tag="I9573_002394"
     CDS             4996..6288
                     /locus_tag="I9573_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAT4797006.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            6724..7362
                     /locus_tag="I9573_002395"
     CDS             6724..7362
                     /locus_tag="I9573_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4797007.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            7369..8211
                     /locus_tag="I9573_002396"
     CDS             7369..8211
                     /locus_tag="I9573_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAT4797008.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            8272..10899
                     /locus_tag="I9573_002397"
     CDS             8272..10899
                     /locus_tag="I9573_002397"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HAT4797009.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            11113..11358
                     /locus_tag="I9573_002398"
     CDS             11113..11358
                     /locus_tag="I9573_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797010.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
BASE COUNT         4578 a         1157 c         2188 g         3539 t
ORIGIN      
        1 tataagaaaa ctaaaagata atttgattaa gaaatagatg ttcgtaaaag agcatctatt
       61 ttttattttt atcagattta gataatttta ataataaaag aaataaaaat aagttcttaa
      121 atttctaaga agaatttaga atatagtcga aataagaatt gtatatttct gaaaattcta
      181 attagtacaa ttttttcctt tataaaattg aaaaaagatg tatttattta tcaaaaatat
      241 tatattaaaa tgatattaat aaaaaaacat ttacaatatt agcatatagt acagaaaaaa
      301 gatgtaattt gtaaaattta tgttaagcag taaaattaaa aatttaaaga tattataggg
      361 aggttataat tttatggaaa acgttattac aggtctaagt aaaggtgcag agtggtttat
      421 cggtttgttt cagaaagggg gagaacaatt tataggtctt gtttcaggta ctcttccaac
      481 gctaattgta ttaatggtag ctattaactc tttgattaag ataattggtg aggaaagagt
      541 taataattgg gcttctaagc taggaaagaa ctcttttaca agatatattc tacttccatt
      601 agtatcagta tttttcttgg gaaatcctat gtgctataca tttggtagat ttttaaaaga
      661 agagcacaaa gcaggatttt atgatgcagc agtttcattt gtacatccaa ttacaggtct
      721 ttttccacat gcaaatgcag gagaactttt tgtatggttg ggaatatcag caggtttgac
      781 tactttaggt aaagaaacta ctacactggc attgtggtat tttatagttg gtctaattgt
      841 aatttttata agaggtatag taacagaaaa aatgtatgct tttctaacaa gaaaaaatag
      901 agcaacaaat aaggcttagg aggtagataa aatggaggag aataaaatat taaaaataga
      961 aaagggaact tcaggctggg gaggtccttt gtatataaaa aaagaaggga atagaaataa
     1021 gatactatca atgactgctg gtggtatcca tgaagtaaca ttaaaaataa aagaactact
     1081 gggttgtgaa attgtagatg gatttaaaac tggagtttct gatgaagaag tagcagtagt
     1141 tattattgat tgtggtggaa ctgctagatg tggagtttat ccaaagaaaa aaatacctac
     1201 aataaatgta aatccagttg gaaaaacagg tcctttagca aaatttataa cagaagaata
     1261 ttatgtatca gatgttaatc ctaactgtat aagtgttgta gatggggaag atatgccaca
     1321 aaaatctcaa gaaaataaaa gcgaaaataa gagttctatt agaaagcctg ataattatga
     1381 tgaggttaag tcaaaagcac aaggtgaata tgctaaaaag aacataatac tttctattgg
     1441 tcaaggggca gggcaagtag tatcaaaatt ttatgatgca ggtcgagaca ctatccaaat
     1501 ggtaatgaat aacgtaatac catttatggc atttgtaagt atgcttatgg gaattatttt
     1561 agcatctgga ctgggagact ggattgcaag agttatatct ccacttgcag gtaatattgg
     1621 tggattattg attatatcag taatttgcac acttccattc ttatcaccaa tactaggacc
     1681 tggtgcagtt atagctcaag tagtagggac tcttgtaggt acacaaattg gtcttggtgc
     1741 aataccagca tatttagcac ttccagcttt atttgcaata aatgggcaag ctggatgtga
     1801 ttttgtacca gtaggattaa gtttgggaga agcagaacca gaaactgtag agtatggtgt
     1861 tccagcttta ttttactctc gtttaataac tggtccaata gctgttatta ttgcatatgg
     1921 agttgcagta tttgcattga gataatattt aatgtcaata taaggcttgg aattaattaa
     1981 taaaattgta aatataaatt taagctagtc aagatttttt gttaatgaaa gactagcttt
     2041 tatactatta attagctgta attatcgagt tagattaata ctaaattttc ctttattaaa
     2101 gtgaaatttt atgtattaat ttggtttcaa atttgtacta gaatgaaatc aaggaggaga
     2161 tacaaaatga gtgtaactga gaaaattcct tctattgacc gcaagatatt taagatattg
     2221 attatgtgta gtaaaaaaga gtttgtgagt attaattcaa tagcaaatga attgaatgtc
     2281 acaactagaa gtgtaaggac gtatataaaa caattgaata aagatttagg taatgatata
     2341 gcagtaatca aatatataaa gggtcaaggc tataaactag aaataaaaga tgagcagata
     2401 ctcaataaga ttattgatat gaatagaaaa aatgtatttt cattaaattc aaaagaagat
     2461 agagtagagt ttatactaaa ttacttgata gaactagatg gatttattac acttgatagt
     2521 ttagctgatg agatgtgtgt aggaagaact actttagtaa atgatttcca gtatgtagaa
     2581 aaagttttgg cttcatataa tttaaacttg attaaaaaac agaatacagg tatgaaatta
     2641 aatggaaatg aacttgatat tagactgttt atattaaatc agttgtataa aaactcaagg
     2701 aaagatttca gtaattcaaa gtattttaaa ggaatcaaaa aagaagaaat cataaattta
     2761 gaagaaaaac tattgacttt atttaaagaa aataattttt atgttacaga tgaaatgtta
     2821 agagaagtta ttaactacat agtagtttta gtctatagag taaaagaaga aaagaaagta
     2881 aaagactatg atgtaaagtt tgatttactt aaatcatatg atgaatactt tattgctagg
     2941 gaaataaaac atataatatc aggaatgttt gaatgtactt taaatgatga agaaattatt
     3001 tatttaacta taccattagt tagtggaaat gcaccagcat cagaatgtgc tttaaatagt
     3061 agtagaatta gtaaaaacat tgatgagttg atggaaaata tatttaatca aatatatgtc
     3121 gatatgggta tttttataaa tgaggatgaa ttaagagtag gtctaggata tcatcttagt
     3181 tttactttaa acaggctatt atttaatata aagttaaaaa atgttttgtt agaagagatt
     3241 aagcaaaatt atatacttcc atttaaatta gcacaaatag caggaaaagt aatagagaaa
     3301 aagtataact tagaagtttc agaagatgag attggatata tagcaatcca tttcagtgga
     3361 tatttagaaa gaaatagcag tagattctat tcaataaaaa aaatagcaat tatatgtagt
     3421 acaggtctag gaacagcaaa actattgaaa ataagagtag aaaaactgat aggaaataat
     3481 cctaaaattg atactctttc aagttttaat ttgagaaata ttaacttaga tgaatacgat
     3541 atagttttta caactattga tttagacaca tcgaatataa atacaatagt attaaaaata
     3601 aatacaatat ttgatgaaaa caaattaaga gaacaattaa aaacagtatt gtgcctaaga
     3661 gaagggaata ttgacactac taattctaca aatttactaa taaataattt gttagatgaa
     3721 gataaattta tgatattaaa tgaaaagact atacttgctt cattagaaaa aatgatggac
     3781 catcttatgg atttgggatg tattgatgat aagtttagaa aaaatatagt caatagagaa
     3841 gaaaaatctc ccacagtatt tgataaaggt ttattatttc ctcactctgt aaatgaaaaa
     3901 tcagataagt ttttaatggc agttggaatt ttagaagaac caatagaata tgcaaataga
     3961 agtataaaaa taattcttat gacaatgttt ccatgtgaga ataagataga ctcagactta
     4021 ttagtaaaaa tttatgaaga agttttaaag ataggccaag atgtaaaatt aacgaataag
     4081 ataagtaaat gtagaacttt tttagaattt aaaaaggtgt tacttaaaaa ccttatataa
     4141 ctattgtaca gcatttatag ttgttataca gatttaaaaa taatgtgatt tttggaaggt
     4201 gtataaatat gaaattatta gtaattttat ttgttgctta tattttaaat actatattta
     4261 taatgaagca gaataaattg tattttaaaa ctttaaatag agctaaaaaa aagggtgata
     4321 tagtgtctac aggcaaaaag aaaagctatt tttctaaagg aagtatagcc attatatctt
     4381 cagattcaga tgggtttata aagtttggag aaattttaaa agggcgtaca gtaatggcta
     4441 aatttaaaga gattgaagaa atagaaggat tagatatata tagtgcagag caaaaattta
     4501 aagatgaaga gagtattgta caagctataa aatttataaa agaaaaaata aatgtttctt
     4561 ttaattaact caatataaat ataaattatc aggaggaatg tcatgaaata tgaagttaag
     4621 ataagtggga taggcagttt agttaaagag ttaatggatg aaagcaattg tcttattata
     4681 tatgatgaaa ccattaatga tgacgactta aaggatatat ctgtaattca ttcgatagca
     4741 acattaaaaa gtgatgttga aataggagat acacttacta tagggaatag agattattgt
     4801 atagtttcag ttggtgatat tgctcagaag actcttagag aaattgggca ttgtacaatc
     4861 aaatttgatg gaaaatgtga agtaaattta ccaggagaaa tacatgtgga aataggaaat
     4921 ccagatataa ctattggaga tttaataact atttcttaat ttgaaaataa aattattata
     4981 acaggaggaa ttggtatgtt agatttaaac agaaaacttg caaaattgga agaggaaggt
     5041 aaaaaaataa aagtagctct tataggagct gggcatatgg gaaatggaat ggtaagccaa
     5101 atggcaaata tgaaaggtat tgaagcttct attgttgtag atataaattt ggagttggct
     5161 cataaagctt ttactgatgc aggaatagat gaagaaatta tagataatgt aacaaatgaa
     5221 aaggatgcag aaataaaatt acaagaagga aaagtattga catgtaatga ctttcttgtg
     5281 gcttgtaaaa ctaagtcaat agatgtagtt atagatgcaa ctgggggtat tgcaattggt
     5341 gcagagatag cattaaactc tattttaaat aaaaaacata tagttatgtt aaatgctgaa
     5401 actgattgtg ttgttggacc aatacttaaa aaattagcag atgatgctgg agtgatattt
     5461 acaggttctg caggagatga gccaggtgca gtaatggaat tatttgattt tgcagatgca
     5521 atggggtttg aagttagagt gataggtaaa ggtaaaaata ataaattaga tttagattgt
     5581 aatcctgata cagttagaga agaagcagaa agaaaaggag cttcacctca tatgatagct
     5641 tcttttaaag aaggaactaa aacaatggta gaaatggctt taatgtgtaa tgcaactgga
     5701 tttgtgcctg atgtaagagg tggtcatggt attgaagcta cagttaatga agtacctaag
     5761 aaatatgcat taaaaagcga aggtggagta ttagataatt atggtgtagt tgattttgta
     5821 aatggtatag cacctggagt tttcgtagta gtagcccata aattaaaagc agttaatgat
     5881 gaactaaaat atttaagtat gggtgatgga cctaattaca tattatatag accatatcat
     5941 ttatgtagtt tagagactcc tttatcagca gctatggcag tcttagacca taaggctaca
     6001 atagtaccta aagctggact agtagcagaa gttatgacta tagctaagaa agatttgaaa
     6061 aaaggtgagt ttatggatgg ttatggagca tatacatgct atggtactat agaaaaatac
     6121 gatgtagcaa aggctatgaa tgctgtacct attggtttga taagtaaaaa gactaaagtt
     6181 gtaaaagata ttaaaaaagg tgaagttata acttacgata tggtagagat agaaaaagat
     6241 actactttat atcatttaag acaattgcaa gaaaaaatat ttgggtaaga tttgatggta
     6301 atataaatag atattagtaa aaagggttga tggttatttt taaatcaact cttttaaatt
     6361 tttatatatt cttgttagga tactttcttt aaaagtatat tataggttat ttgtagtcgt
     6421 aaaaattatt ttgaattagt tctgcaattt tatattcaca ttgaaacaat attattaaaa
     6481 attttaatat aaaatgaatt aaaatgacca attatgtatt ataatattaa ctgtaatact
     6541 aatatagttc tattaattaa agaaatatcc cccattaata tataataaat atctttaagc
     6601 gagagaactt agtaaaaaat atatcgtatt caataaaaaa tctgtttaca gaatggtata
     6661 tatagttata taatatacta tataagatgc gaaaagataa aaagtatagc acaatgaaag
     6721 aaggtgatta ttattcagct taatgaaaga caactaaaaa ttatagatat agttaaggaa
     6781 aatgaaccaa taacgagtga aagtattgcc tccagcttga atgtgactcg tgctacactt
     6841 agatctgatt tagctatatt aacaatgaca ggaatattag acgcaagacc aaaagttgga
     6901 tatttttatt caggagttag tgaaataaat ttaattggta aaagtgtaaa agaaaaaaca
     6961 gtagaggata taatgagcat gcctgtacta gctaagaagg atgaaagtat atatgatgta
     7021 atagtcacta tgtttttatc tgatgtaggg agtatagtta ttatagatga gaatgaagag
     7081 ttatgtggag ttgtttccag aaaagacttg ctaaaagcaa ctataggagg ttctgatata
     7141 aataaaatgc caataggtat gataatgaca agaactccaa atgtagtcac tttaactaaa
     7201 ggagcaagtg ttttattagc ttcaagaaaa ataatagaac atgaagtaga ttcgattccg
     7261 attgttgaat ataagggaga agacaaaaat catatgagag ttgttgggag aatatcaaaa
     7321 accaacataa ctaagctatt cttagaaata gttgacaatt aaggaggtat gctggtgaaa
     7381 aacctaatta tatatgcagt atcagattca gtaggggaga ctgcacaaca agttgcaaag
     7441 gcatgtatgt ctcagtttta tgtgaatgaa acttatgaaa taaaaagatt tccatacatg
     7501 attaataaag gagtactact tgaaactttg gagaatgcaa aagctgaaaa cgctttaatt
     7561 gtatatacat tagttgatga agaattatgc agtattgttg aaagatattg cgaaagagaa
     7621 ggtcttagtt gtatagattt aatgacagat atactaagag aaataagcaa gagaacagga
     7681 agaaaaccta aaagagaagc tggtataatt agaaaattag atgaatctta ctttaagaga
     7741 gttgaagcta tagaatttgc agtgaaatat gatgatggaa aagaccctag aggtgttctt
     7801 caagcagaca taattttagt gggaatatca agaacatcta aaactccttt gagtatgtat
     7861 ttagctaata aaaatataaa agtagctaat gttccattag ttccagaaat accaattcct
     7921 aaagaagtgt ttgagataga tactaaaaag ataataggtc ttactaattc acctgaaaag
     7981 ctgaatgaga taagaaccca aagattaaaa gctttaggat tgtccagtaa agcaaactac
     8041 gctaatttag aaagaatact tcaggagtta gattattcag aagagataat gaaaagaatt
     8101 ggatgcccag taataaacgt ttcaaataag gcaatagaag aaacagcagg tataatttta
     8161 gacataatga aagaaaatgg tctaaagata tacaaagaaa tagaaattta ataatgtcta
     8221 ttaataaaaa tgatataaat taaaataaaa agattaatgg gggatttaac aatggaaact
     8281 aagtatgttt atagttttgg tgaaggaagc aaagacatga aatctttact aggaggtaaa
     8341 ggtgccaatt tagcagaaat gactaagata ggcttacctg tccctcctgg attcactata
     8401 acaacagaag catgtaatga ttattatgtt aacaatgaaa gtataagagc agagataata
     8461 aaagaaatag aagagcattt agctacttta gaaaaagact taaataaaac attaggatgc
     8521 aataagaatc cattattagt atcagttcgt tctggtgcag tattctctat gccaggaatg
     8581 atggatacaa ttcttaacct aggtcttaat gacaatagtg ttgttggctt agcagaggct
     8641 acacaaaatg aaagatttgc atatgatagt tacagaagat tcatacaaat gttttctgat
     8701 gttgctatgg aagttccaaa atataaattt gaaaatgtat tagacagagt aaaagaagca
     8761 aagggataca cagttgatac agaacttaca actgatgatt taaaagaaat agtaaaagaa
     8821 tttaaagcaa tttataaaaa agaaataaaa aatgatttcc cacaagaccc aaaagagcaa
     8881 ttaatgcttg caatagaggc tgtatttaga tcatggaata atcctcgtgc aatcgtatac
     8941 cgtaaattga atgatatagc tcataactta ggaacagcag taaatattca atcaatggta
     9001 tttggtaata tgggagaaac tagtggtaca ggtgttgcat ttactagaaa tccagctaca
     9061 ggagaaaata aattatttgg tgaattttta atgaatgcac aaggtgaaga cgttgttgca
     9121 ggtataagaa ctcctcaaaa tatatcaact cttgcagatg taatgccagc tgtattcgat
     9181 gaatttgtaa aaattactca tatacttgaa ggtcattata aagatatgca agatatagag
     9241 tttactatag aaaatgaaag attatatata ctacaaacta gaaatggtaa gagaacagca
     9301 gcagcagcaa taaatgtagc ggttgattta gttgaagctg gaataattga tgaaaaagaa
     9361 gctatcatga gaattgagcc taatcaatta gaccaattat tacatccaaa atttgaagat
     9421 aaggcattaa aagaagccaa ggttatagca aaaggtttac cagcatcacc aggtgcagca
     9481 agtggtaaag tttatttcaa tgctgatgat gtagtaaaag caaatgaaaa aggagaaaaa
     9541 gtagttttag ttagattaga gacatctcca gaagatatag aaggaatggt taaagcagaa
     9601 ggaatcctta cagctagagg tggaatgaca tcacatgcag cagttgttgc tagaggtatg
     9661 ggtaaatgtt gtgttgctgg atgtggagaa ataaaagttg atgagtttaa taaagaaata
     9721 agagcacttg atgatgtagt tataaaagaa ggtgaataca tttctataga tggttctaca
     9781 ggaaatgtgt acctaggaga tgttaagaaa acagaagtat ctttaactgg aaactttgaa
     9841 aaattaatga actgggtaga caaacacaaa tgtatgatgg ttagaactaa tgcagataat
     9901 cctagagatg ctcgtgcagc tatagagttt ggtgcagaag gtataggtct atgtagaact
     9961 gagcatatgt tctttgatga agctagattg ccagcagtta gagaaatgat attatctaat
    10021 acagtagaac aaagagaaaa agctcttgaa aaaatattac caatgcaaag agaagatttt
    10081 gtagagttat ttaaagttat ggatggaaaa ccagttaata taagattact agacccacca
    10141 ctacatgaat ttttgcctca tgatgatgag actatagaag aactttcaaa atctatggga
    10201 ataaaagtaa gtgatattaa gaagagaata gttgatttag atgagtttaa cccaatgctt
    10261 ggacacagag gttgtagact tgcaataact tatcctgaaa tatgtgtaat gcaatctaaa
    10321 gctataatac aaggtgctat agaagcaatc aaagcaggtg ttaaagtttc tccagagata
    10381 atggttccat tagttggaga agtaaatgag ttaaaaataa taagaaaaat gatagtagaa
    10441 actgttgacg ctataataaa agaagaaggt atagaagttc cttatactgt aggtactatg
    10501 atagaaatac ctagagcttg tttaactgct gatgaaatag cacaagaagc agacttcttc
    10561 agttttggta ctaatgactt aactcaaatg gcatttggat attcaagaga tgatgctggt
    10621 aaattcttag gtcaatatgt agatgaagaa atattagaaa aagacccatt ccaagtacta
    10681 gaccaaaatg gtgttggtaa gttagttaag atgggagcta aattaggtag agaagttaaa
    10741 ccagaattaa aactaggaat atgtggagag catggaggag aaccatcttc agtagagttc
    10801 tgttatagtg taggacttaa ctatgtatct tgttcaccat ttagagtgcc aattgctaga
    10861 cttgcagcag ctcaagcatc tataaaaaat cctagataat aatagactgc aaaatatgta
    10921 actaaataaa aataaaatcc cctattgttc aatgtatcaa attaagtatt tgtaaattga
    10981 atatagggga tttttattat aaaaatttaa cttatacagt tgtaagtgaa acgtatttaa
    11041 ttatattgca tatattattg tagagagaaa tttcaactat caattaaatt gagtttactg
    11101 gaggggataa agttgacaaa gtgtcctaaa tgtggaaagg aagtatcatc acgtccaggt
    11161 agtatatgcc cacgatgtgg atttaaagta gcagatgaga gtctaaaaat aagatgtcca
    11221 gaacctagtt gtagggcttt ggtatctagg aaactggatt attgtcctaa atgtggatgc
    11281 aaacttagag gatataatat ttctgaattt gttaacatgg taagatgtaa aatagatgag
    11341 acatttgata aaaaataatt aaatttagag ttaagctttt gagggtgtct caaaatgaac
    11401 tttttagttc agaaggcact ctttttgtat ggaatcaaat atatttttat aatttaaaca
    11461 at
//
LOCUS       DACTGW010000122        11438 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.122, whole genome shotgun sequence.
ACCESSION   DACTGW010000122 DACTGW010000000
VERSION     DACTGW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.122"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            46..363
                     /gene="flgB"
                     /locus_tag="I9573_002399"
     CDS             46..363
                     /gene="flgB"
                     /locus_tag="I9573_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAT4797011.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            371..775
                     /gene="flgC"
                     /locus_tag="I9573_002400"
     CDS             371..775
                     /gene="flgC"
                     /locus_tag="I9573_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAT4797012.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            785..1102
                     /gene="fliE"
                     /locus_tag="I9573_002401"
     CDS             785..1102
                     /gene="fliE"
                     /locus_tag="I9573_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAT4797013.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1127..2677
                     /gene="fliF"
                     /locus_tag="I9573_002402"
     CDS             1127..2677
                     /gene="fliF"
                     /locus_tag="I9573_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
                     /protein_id="HAT4797014.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAIPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDEDVDENNQPTNSLINVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKAEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2690..3760
                     /gene="fliG"
                     /locus_tag="I9573_002403"
     CDS             2690..3760
                     /gene="fliG"
                     /locus_tag="I9573_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAT4797015.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRDESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPALAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3753..4484
                     /locus_tag="I9573_002404"
     CDS             3753..4484
                     /locus_tag="I9573_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAT4797016.1"
                     /translation="MNNKIIKSKKASYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4488..5807
                     /gene="fliI"
                     /locus_tag="I9573_002405"
     CDS             4488..5807
                     /gene="fliI"
                     /locus_tag="I9573_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HAT4797017.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGIAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            5839..6285
                     /gene="fliJ"
                     /locus_tag="I9573_002406"
     CDS             5839..6285
                     /gene="fliJ"
                     /locus_tag="I9573_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HAT4797018.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIESNLE
                     NLESNYSKYSISKNNDSVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6313..7518
                     /locus_tag="I9573_002407"
     CDS             6313..7518
                     /locus_tag="I9573_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HAT4797019.1"
                     /translation="MKIQADSYNIIKDIGNRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRRETSKQNVSSDKRENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALHQLLNLLK
                     DNYKKDKDINTILNNLKKLSISDELKQELKLNKNILNKIANQNLELNRASTNNPNDIE
                     NLDIMKLRELLENNASEDIEVISKTTENDTKSIDLYSFDSNRIDTLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            7536..8144
                     /locus_tag="I9573_002408"
     CDS             7536..8144
                     /locus_tag="I9573_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HAT4797020.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEEEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDANSGEENDSSEQESPEEV"
     gene            8171..9154
                     /locus_tag="I9573_002409"
     CDS             8171..9154
                     /locus_tag="I9573_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HAT4797021.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGVIYDGEYKADT
                     GEFIKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GNIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9167..9370
                     /locus_tag="I9573_002410"
     CDS             9167..9370
                     /locus_tag="I9573_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HAT4797022.1"
                     /translation="MIRVTDLNGRRYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKVNVGYIRSEK"
     gene            9370..10188
                     /locus_tag="I9573_002411"
     CDS             9370..10188
                     /locus_tag="I9573_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Homolog of MotA, appears to be involved in motility
                     on surfaces and under different ionic conditions. With
                     MotS (a MotB homolog) forms the ion channels that couple
                     flagellar rotation to proton/sodium motive force across
                     the membrane and forms the stator elements of the rotary
                     flagellar machine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HAT4797023.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEVNEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10178..10873
                     /locus_tag="I9573_002412"
     CDS             10178..10873
                     /locus_tag="I9573_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAT4797024.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGISMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKADSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10885..11328
                     /locus_tag="I9573_002413"
     CDS             10885..11328
                     /locus_tag="I9573_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HAT4797025.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
BASE COUNT         4814 a         1217 c         2263 g         3144 t
ORIGIN      
        1 attaataagc aaaataaata aaatatattt tgaggaggag tgagtgtgaa tacatatgac
       61 attataaaaa tgggattaga tgcaactaat ttaagaagtc agacaatagc aaataatata
      121 gccaatataa atactgaagg atataagaga cattatgtaa cttttgaaga aacattaaat
      181 gatgctatgc caaaaataga agaaaaaaca gacaattcta aaggattaag agtggatggc
      241 aataatgtag atatagaaga agaaaaagta aatcaagcaa tggcaacatt acaatataat
      301 gctttgataa gttttgcaaa tggaaaaata gcaatgacta aatcaataat aagtgggagg
      361 taattaaaag gtgagtgtgt tcagtggaat gagaatatct gctagtggat taacagcaga
      421 aagcttgaaa atggatatca tatcgtccaa tgtagctaat ataaatgcca caagaggtcc
      481 aaatggagaa gcatatagga gaaaagttgt ttcatttgaa gaaaactatg ataataaatt
      541 aggtatgcta ggagtaaaag ttacatctgt tgaagatgat aaatcacctc ttaaagaagt
      601 atatgaccca tctcatccag atgcaaagcc aaatggatat gtagaatatc caaatgtaaa
      661 tatattggta gaaatgacag atttaataac agcttcacga gcatatgatt ctaacgtaga
      721 tacattgaat gctcagaagt ctatgatatc aaaagctttg gaaataggaa agtagggata
      781 aattatgcca attacacaaa ttaacaattt aatgcaaggt gcaaagttag ataatttgat
      841 tggagaaaca aaaccattaa ctcttaataa taaagaagaa aaaaagaact ttgaagatgt
      901 aataatagat actatagata aagttaattt tcaacaagtg acagcagatg atatgaaaga
      961 aaaatttata aaaggcgaag atgtttctat gcatgaagtt atgttaatag gtcaagaagc
     1021 acagctatct atgcaatatt taatagaagt tagaaataag attcatgatg catatcaaga
     1081 aatgaataag gtacaaattt agccttttga acaggagtaa taatcaatga tagccagttt
     1141 taaaaataag attaaagagc aagttgctaa atctaggaca aagataagtt ctctaaaaaa
     1201 gggaactaaa atagcattag gtataggggt tattgctata tgtttagcgc tagtatttac
     1261 agcaatatct gcgagcaaaa ataagtatgc agtcttattt tcagaactag atgttcaaga
     1321 tgctgcagat atatcagcag aattagagtc taaaaagata gatactaagg taaagggaag
     1381 tacgatttat gtacctagaa cagaagttga taagttaagg ctggagctag caccaagtct
     1441 tacaggtggt tctaaaggat ttgcactaat ggatgatagt tcatcaatag gacttactga
     1501 tgaagagttt cagattaaga agcaaagaat gatacaaggt gaaatagaaa aaacaataaa
     1561 gagttttcca caagtggaag atactagagt acatataaca tttggagagt catcagtatt
     1621 tgagaaggag gctattccag ggagtgcagc agtttatttg atgttaaaac cagggacgac
     1681 tttggaaaaa aaccaaataa aatctgttat ggcacttata tcaggaagtg ttacaaatat
     1741 accagagaaa aatgttcaag tcattgataa caaaatgaat ttattatctg aaggtatttt
     1801 agaggatgaa gatgttgatg aaaataatca accaacaaat agtttgatta atgttgataa
     1861 aaataaggca tctgaacaag cattgaataa acaattagaa aagtctattt tagaaatgct
     1921 agaaccaata tttggtaaag gaaaagtaaa agccacagta aatgctgatt taagttttga
     1981 tacagctgaa aaaactgaga taaaaattga ccctgataaa gttgcaataa aggagacaag
     2041 gtcgaaaaat tcatctaaat ctgctacaaa taatgttcaa ggtgtagatg ctaacatgaa
     2101 taaccaagga aataacaatg gtactaatac agaagatagt ttagatgaga gttttgagta
     2161 tgaaacaggt aagattgaaa ctcacactat agttgcgcct ggagaactta atagattgac
     2221 agcttctgtt gcaatagatg gaaaaatagc taaaggtgta caagctgatg tagaggatat
     2281 tgttaataat gctataggta tggatggagc gaggggagat actcttagcg ttgtttcaat
     2341 ggtatttgac ccagaaggga aggcagaagc taaacaagag ctagaagaga ttaaaaaaga
     2401 aggatttaaa aagaacataa taaaagcagt tattggagta gtggcagtgg tagcaattgg
     2461 agctattgga tacttaatat ataagaaaag aagtgaacga gaagatatgg attatgaaga
     2521 tgaagattat atttctaaat ctgattctgt tgataggaag gataatcctc tattaggaga
     2581 tgacccagaa ctgacattag aggaagctgt taaaatgtat gcagaagaaa agccagacca
     2641 agtaacagag ataataaaaa cttggttaaa tgagtaaggg ggatagaaag tgtccacgga
     2701 aattacaaat gttaatgcgt ttgacttaag tgatataaat gaaagtgata agaaagagaa
     2761 gatagaaggt gagggtttaa aaaaagctgc tgtattatta atgacattag gaccaaatgt
     2821 gtctagtgat ataataaaaa gcttgcctga taaacaagtt caaaaaatag gtttagaaat
     2881 tgcaaatatt tcttcaatag cttcaaaaga aagaagagaa atcttaaaag agtttgtaga
     2941 gataaatagg gcaaaagatt atgttataga aggtgggctt gaatatgcaa gagagttgtt
     3001 aagcaatgct ttaggaaatg ctaaagcaaa caaattactt gaaggtatat caataaatac
     3061 aagtacaaag ccttttaaac atattataaa actagatgta aagaaaatat tagaaatact
     3121 tagggatgaa agtgcacaaa ctatagctgt tttacttgca catatggagc cagaaaatgg
     3181 agcagaagtt ttatcatact tagattttga tttacaaaat gaagtagcta tgagaataag
     3241 ctcaatgggt tcaatatctc cagaagtaat aaaggtaatt gaaagttcat tagaagataa
     3301 attacactca ataagtggtg atgattttaa ggattatgga ggagtgtcta ccttagttca
     3361 aatattgatt aattctgata gaaaaacaga aaagaatatt ttgggaacta ttgaagaaca
     3421 agacccggct ttagcagctc aaattaaatc tcatatattt gtctttgaag atattgtcaa
     3481 gttagatgat ataagtatac aaaaagtatt aaaagaagtg actatgaaag atttagtata
     3541 tgccttaaag ggtgtggatg atgagatttc agatgctata tatagaaatc aatcttctag
     3601 agcagcagat gcacttaaag aagaaatgga aatggttggt gtcataaaag ttacacaggt
     3661 tgaggctgct caacaaaaga tagcaaatat agtaagaaaa cttgaaagag aaggtgccat
     3721 aactattgta aggaattttg gtggtgactt tgttgaataa taagattatt aaaagtaaaa
     3781 aagcatcata taaaggagtg ctagatttaa ccataataga taaagttaga gtgggagaat
     3841 caaatgtaaa taaagaaaat gattgtagat ttagagatga ggtaataaaa gaagctatag
     3901 aatctgctga gataaaaaag aatgagatat tagaagaagc aaaaatgaat gcacaaaata
     3961 tagaaaaaca agcatatgaa aaagggtata gtcaaggtca aaaaaatgga tatgaagatg
     4021 gatataaaga gtcatatgat gaatataccc aaaaagctaa agatgaagca gattttataa
     4081 aaaatcaggc agacttaata ctattacaag ctaatgagaa gatgactgag tacataaaag
     4141 aaaaaaataa agagattgta agacttgcta ttaatatggc tagttctgtt ctaaaaaagc
     4201 atttcgaaga tgaagaatct atgagtgatt tgttaaagca aattattcaa gattatgaag
     4261 gaagtagttc atttatagta agatgtaatt ctttctataa agataatata gagaaagagt
     4321 ttgaattatt caaagaggaa acatcactaa aatcaagtgt atttgttatt gcagatgata
     4381 gtatagataa aggcaatgct atcatccaaa aggaaaatgg aagaataata gttggtatcg
     4441 attgtgctat ggaaaaacta aaagaagaat tgcttggtga ataaagtatg tataaaactg
     4501 atatatctat agattttgac aaaataaata agaaaataga aaatttagaa ctcactatat
     4561 cagaaggtag agtaaagaag ataataggac ttactgtaga agttgaagga ataaaagctt
     4621 ttgttgggga gttatgtgtt atatacaatc aagtcaataa accagtgaat tgtgaagtag
     4681 tgggttttaa agataaagaa gtcatattaa tggcgttggg agagctaaca ttgatagctc
     4741 ctggttgtaa agttatttct aagggaatac ctttaagtgt tatgtgtagc gataatttac
     4801 taggtaaagt acttgatggt cttggaaatc ctatagacaa ctcagatgct gttttaggag
     4861 atagatacaa cttgaacaat gaacctcctg acccaatgaa gagaaaaaag atacgaaata
     4921 taatggaaac aggtgtaaga gcaatagatg catttactac ttgtggtgaa ggacagagaa
     4981 tagggatatt tgcaggaagt ggagtaggta agagtacgac tctaggtatg attgctagga
     5041 atgcaaaagc agatgtaaat gtaattgccc ttattggaga aagaggaaga gaggtactag
     5101 attttattga taaagattta ggtgaagagg gaatgaaaaa atctgttgtt gtatgtgcaa
     5161 catcagataa agctcctttg gttaggctaa aaggtgctct tactgctact gcaatagcag
     5221 agtattttag agaccaggga aaaaaagtaa tacttatgat ggattcagta actagatttg
     5281 ctatggcaca aagagaagtt ggtatagcaa taggagaacc accagctcaa aaaggatata
     5341 caccatctgt atttgctatt ttaccaaaac ttatggagag aacagggaca tcagataaag
     5401 gttctataac tgcattttat acagtattag ttgatggaga tgactttaat gagccaatag
     5461 cagatacaac aagagggata cttgatggtc atatagtttt atctagagac ttggcaaata
     5521 agaatcatta tccttctata gatgtattga acagtttaag cagacttatg aatgagattg
     5581 cttcaaaaga agacataaaa atagcatctt ttgcgagaga tatgcttgct gagtatagag
     5641 aagcagaaga tttgataaat ataggtgcat atgcttcagg tacaaacaaa aaaatagatg
     5701 aagcaatcta ctatcatgag catattatta attttttaaa acaaggaata aatgaaaaga
     5761 gttcttttaa tgaaactata agtagtttaa gaagaatctt tgaataaaca atttaaaaaa
     5821 atatttgaat aggttgtgat ggaattggat aaaaatttta agtttagatt acagaaagtt
     5881 ttagacttaa aaataaaaga tgaagaagag ataaaaatgg aatttgctaa gattcaacaa
     5941 aaaaaaatag atatagagag caatttagaa aacttagaaa gtaattacag caaatattct
     6001 ataagtaaaa ataatgatag tgttcaaaat caaaaaataa ctattaacta tttgttagct
     6061 ttaaacaact ctataatgga tttatcagaa gagctggata agtcgacaaa tgaacttgaa
     6121 aaagctagaa agcagcttat aagcaaacag atagagagaa agtcattaga aaaattaaaa
     6181 gaaaaaaaat atggacagta ttataaagaa gaacagttaa aagaacaaag cactaatgat
     6241 gagtttgcaa gcatgagtta tttgagaaat agacaagttc tataaaatag taggaagtga
     6301 ggatattatt gtatgaagat acaagcagat tcttataata ttattaaaga tattgggaat
     6361 agaaaaagct catctaagaa taaaaatata aaaaataata aatttgaaga taatctatat
     6421 aaggcaacag accataaaaa ggttaaagat agaagagaaa caagtaagca aaatgttagt
     6481 tcagataaac gggaaaatag tgataatgag tgtcatttag atgaagataa aagtaatcac
     6541 ttagaagatg aaaatacata tgaaaaagat tgtagtaata ataatgaagc tttacatcaa
     6601 ttactgaatc ttttaaaaga taattacaaa aaagataaag atataaacac tatattgaat
     6661 aatctaaaaa aactaagtat atcagatgaa ttaaaacaag aacttaagct taataaaaat
     6721 atattaaata agattgcaaa tcaaaattta gagttaaata gagctagtac aaataaccct
     6781 aatgatatag aaaatttaga cataatgaag ttaagagaac ttttagaaaa taatgctagt
     6841 gaagatattg aagtaattag taaaactaca gaaaatgata ctaaaagcat agacttgtac
     6901 agttttgaca gcaatagaat agatactctt aactcaaata aacagaaaga tgatagttct
     6961 gatattttgg aaaaactagc aggggtaaat ggaaataaga gtggaaattt tggacaagta
     7021 ataaataaaa gcgttgatat gaataaaatt aaaaatgata atgttaattt aaaacagata
     7081 gaatcaaatg taatggatga ttctataaaa gctattaagc atatgaaaac aaatgatata
     7141 caagaattga caattaaatt aagaccaaaa gaattaggag atatgaacat acaattgtta
     7201 aaagatggtg aaagtatgag agctgttgtg actgttttca acaaagatgt ctttgactct
     7261 ataaataaaa acatagcaga tttaaagcaa catcttgaac ttacaaatgt aaatataaaa
     7321 gatgtatcag tgcaaatgca tagcgataat acaaatacat ctgatacctt tgataaagca
     7381 tttgaacaac agaatagaca aaataaccaa gaaaacatga gtaagcaaaa tacaaatgat
     7441 aagcgtaaca atgtagtcat agaagatgag attaaagaaa atattattga tgatgataga
     7501 gtagatttac tagcttagta agggggaagt attgcatgta cggaatagca acggctttgg
     7561 caggaactca ggtaaactat aggtcaaata atttaagcca acttgaaaga aatattaatg
     7621 atattgccaa taaacaagtt agaagtaaag gaaatcagac agtaacagac aatgggactc
     7681 caattatagg acaaggcgat gatgctgata aagatttgtt tatgaagatt cttgtagcac
     7741 agatgggtaa tcaagaccca atgaatccac aagacccaac agaatatgta tcacaattag
     7801 cacagtttgc aactttagaa aagatgtcag ctatgaatga taagctagat atacttttag
     7861 ccttaagcaa ttccactcta ataaattctg ctttgagtat ggcaacatca ttaattggta
     7921 aagaagtaga agtatctggg gaagaggaag aagatgaaaa cattgtagga gtagtaaaat
     7981 ccacatatat aaaagatggg gaagtacact tagaattaga agtagatgga agtgacaaaa
     8041 ctgtaggtgt taaatatgaa aaattactta aaataggaga ggtagatgca aattcaggag
     8101 aagaaaatga ctcttcagaa caagaatctc cagaagaagt ataatattaa atgagaaaag
     8161 aggtaaattt atgataaaag caatgtattc aggtgtaagt ggaatgaaag caaatcaaac
     8221 aaaactagat gtaataggta acaatgtagc caacgctgga actacttctt ttaagaaaag
     8281 ttcagctaga ataacagatt cattttatca aactgtacta tatgcaagtg ctccaacagc
     8341 ggcattaggg ggaacaaact taggacaagt aggtgtaggt agtaagatat caagtataaa
     8401 taaagatatg acacaaggaa atgtacaacc aactaatagg ccatctgatt taatgataga
     8461 tggagatgga tatcttccag tagtaagaca aggaacagtt atgtatacga gagatggttc
     8521 ttttaattta gatacaggat ttaaagcagc tgatggtgct ttgaagaata ttaaaggtgg
     8581 aagacttgta aatggtgatg gatatatgtt acaaggtgtg atttatgatg gggaatataa
     8641 agctgatact ggagaattca ttaaaaaggg tgatgctaaa ggaaaagagc ctattgtaat
     8701 accacttcaa agtataagtc ctaaggatgg aacagttcaa aatgtactag agtataatat
     8761 atcaaaggat ggaacagtag aatttttatt gagtgatggt caaagaactc aatttgtcta
     8821 tgatatacct gatggtgcta aggtaggaga tacacctgct ggaatagata aattaaaagg
     8881 tacaatacaa aaagtgcaaa catatgcatt ccaaaatcca gcaggactag atgcagaagg
     8941 aggaaactta ttcaagccat cagcaaactc aggagatcca caaatagcag gagctacagg
     9001 aaacataata caaggggcaa tagaagcttc caatgtagat atagcagaag aatttacaga
     9061 aatgataata gcaagtaggt ctttccaagc aaacagtaaa ataatatcga catcagacga
     9121 aatacttcaa gaaataataa atcttaagag atagtaggta catattatga taagagtaac
     9181 ggatttaaac ggaagaagat acatattaaa ttcagacctt atagtgagaa ttgactctgt
     9241 accagaaagt aggattctcc ttacaacagg agaaaaaaat cttgtaaaag agtcagtaga
     9301 tgagatagta gataaagtaa taaagtataa aagaaaagta aatgtaggtt atattaggag
     9361 tgaaaaataa tgaaaaaaac agattcacta acattcattg gactaatatt atctacagta
     9421 ttagttttat ttggggcagt aaaaggaagt agttcaggat tgaaaagttt tttcgatttt
     9481 gcatctatat taatcacagt atttgggtca tttggagctt tgatgattac atttactatt
     9541 gatgatataa aacttataaa agatgcgcta caatactcat tcaagacaat gaatatatcg
     9601 aaattagact tattaaatca atttaaaaca ctttctaaaa aagcaagaag agaaggactt
     9661 ttatctatag aaagtgatgt aatgaaaata caagacccat ttatgaaaaa agggttagag
     9721 ttatgtattg atggccttga agtaaatgaa ataaggtcta tactagaact cgaaatagat
     9781 tctgttgaag aaaccaataa cagatattct aaactattta aaacttgggg gactttcgca
     9841 cctgcatttg gtatgttggg aactctaata ggtttaatac aaatgttatc tgaccttaca
     9901 tctccagatt taatagcatc gggtatggga aaagcattaa ttacgacatt ttatggttct
     9961 ttacttgcta atatcgcttt gaacccaatt gcatataata tagatgagaa gactgaaaaa
    10021 gagatttatg taaaggaaat gatgctggaa ggtataataa gcatacaatc tggggagagc
    10081 agcatagtag ttgaggaacg tttagctaca tacctttcaa acaatgagaa gctagaaatt
    10141 atgaaatcca ataaaaatac agaaagagca atgagtaatg gcgcgtaaaa aaaataagaa
    10201 aaaggatgag ataaatccag atgcatggtt ggctacttat gcagatacta taacactgat
    10261 tcttacattt tttgtgttat tgtatgctac atcagttgta gatatggaaa aatttaaaaa
    10321 catagcagat gcgttaaaag gtcagttttc tgggataagt atgttcagta caggttcaag
    10381 tacaagtgag aagtctccaa tagagagtgt tttagtggat aaaaatgata agtcaaattt
    10441 agaacaggat ttaaaagata aagttaataa ttctaactta caaaatagtg taacagtcaa
    10501 agatgatagt agagggattt tactagaact agatgattca attttatttg actctgggat
    10561 ttctgaatta aaagcagata gtaaagccac tcttaataaa gtatatgaca tgataaagat
    10621 gatgaaaaat aagatagtta tagaaggcca tactgataat gtacctgtaa aatcaagtac
    10681 acatgaatcc aattgggatt tatcttctag tagagcagta agtgttgtaa gatactttgt
    10741 agaagaaaag ggcatggacc ctagactatt ttcggcaaca ggatgtggag agtatcaacc
    10801 tttggtttca aatgaaacag caaagggaag ggctgagaat agaagagtta atatacttat
    10861 aactattgac taggaggaag aggtttgttc aaaaataaaa aaattattat aattattttg
    10921 atactgatta ttttctcagc aggagcaggt ttttttatat taggagaagg tggaaataaa
    10981 caaattgaca agattgcagg tttgtttatt ccaacaggga aaggcaaaga tgaagtaaag
    11041 accgaaaatc ttgacttaga agagatggta ttaaagctag ctgatgaagg tgtaagatat
    11101 ttaaaagtat caataactgt atcttatgat tcttcatata aagatataga aaaaaatacg
    11161 cctttaatta gggataatat tataaattgt tttatggcaa aaagggcaga tgattttact
    11221 gctgataatt taaagaacat aaaagaagaa attaaaaata cattaaatat acaactaggt
    11281 gaaaatgtaa ttcaaaatat atatttcaca aatatagtag tgcaataaaa ggatggtaag
    11341 acatgatact ttatttacta aaaatattaa tatgtatccc aataatttta atattgatag
    11401 tcttatcttt aaaaattagc caaggaactt tattaaaa
//
LOCUS       DACTGW010000123        11410 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.123, whole genome shotgun sequence.
ACCESSION   DACTGW010000123 DACTGW010000000
VERSION     DACTGW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.123"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            309..1010
                     /locus_tag="I9573_002414"
     CDS             309..1010
                     /locus_tag="I9573_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HAT4797026.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKSALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLLVVFILGHRMRGIVEKMNVKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAVISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIVLS"
     gene            1036..1692
                     /locus_tag="I9573_002415"
     CDS             1036..1692
                     /locus_tag="I9573_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797027.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGLRFGQMPMLSVIFQI
                     WGLVATTYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFITDSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDSNKTKE
                     "
     gene            1899..3539
                     /locus_tag="I9573_002416"
     CDS             1899..3539
                     /locus_tag="I9573_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HAT4797028.1"
                     /translation="MFDVKIANGIIIDGTGNSRYKGDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPIKLNLHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDKVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWDGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            3898..5220
                     /locus_tag="I9573_002417"
     CDS             3898..5220
                     /locus_tag="I9573_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797029.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMLKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDICCC
                     ITGLEEAYHAMKKENIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPAVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAI
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            5417..6424
                     /locus_tag="I9573_002418"
     CDS             5417..6424
                     /locus_tag="I9573_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HAT4797030.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGTMGCPPEILKKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            6462..7859
                     /locus_tag="I9573_002419"
     CDS             6462..7859
                     /locus_tag="I9573_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HAT4797031.1"
                     /translation="MASTNKNNVTSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKINTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIFKGEDN
                     MSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKITHNIWE
                     EKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIWSNFGA
                     DGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDNLWIVT
                     QLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEMMLEMT
                     SRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYEVERVT
                     CNQREKNEGYDVSIRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKEEWITF
                     DGMDVLKQMYMGVEE"
     gene            8004..9269
                     /locus_tag="I9573_002420"
     CDS             8004..9269
                     /locus_tag="I9573_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4797032.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTDIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            9311..10350
                     /locus_tag="I9573_002421"
                     /pseudo
     CDS             9311..10350
                     /locus_tag="I9573_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
     gene            10385..11407
                     /locus_tag="I9573_002422"
     CDS             10385..11407
                     /locus_tag="I9573_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAT4797033.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMVAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
BASE COUNT         4114 a         1297 c         2286 g         3713 t
ORIGIN      
        1 ataataattt ttttaaaata acaaagtttt ttgattaaaa gttttatatt gaaatgtcaa
       61 agtaataaaa taccttataa gttaattaca ggttaaaaaa ataatatgtg aattttctga
      121 tatttactaa ttatcaataa agtatttaag tattaatgtt tttagtgaga ctttaagaca
      181 gataatctcc tattaaagat actatatctt agataaaata aattaaaaca atataaaaac
      241 ataacaagat tacagaaaga aagctcataa taaaacacta aaaaatttat tttaaggagg
      301 aataaagtat gaagtttcaa gaaatgatta atagcccagg tctttggatt gtttctagtt
      361 ttttagtaat tatttcagtt gttcaagcaa tcgtatttat gaaatcagca cttaaagagg
      421 cttcaaatct tggtattgaa agaaaaggaa ttaaagcagc aattcgctca gctagtgtta
      481 cagcaattgg accctctcta tctcctgtta ttacattatt atcactggta gctgtgattg
      541 gtgctcctac gacttggatg aggctttgtg atgtaggagc tgccagaaca gaacttggag
      601 taatatctct tacatcaaat ttatctggag tagaagtagg tagtgctgca tttggagcag
      661 aagctttttc gtatgcacta tggggaatgg ctttaaataa cttaggttgg ttgcttgttg
      721 tatttatttt gggtcataga atgcgtggaa ttgttgaaaa aatgaatgtg aaatataatc
      781 ctacttgggt taaaaaacta cttgcagggg ctacagttgg attatttgct taccttcttt
      841 caaatcaaat aaaaacattc gaaataccaa aacttacacc agcagttatt tcagctatag
      901 ttatgctagt attgacgact ttatttaaaa agaatcaacg attacaagaa ttatccttag
      961 gtattgccat gttgattggt atgtttggca cacagattgt actatcatga ttaaaacaga
     1021 taagaggtga taattatgta tgataaaaag atgataaaaa tagggagaat ttctataggt
     1081 cttgcaatta ttgcaaattt tattcctgca atctatgtag gtcttcgctt tggtcagatg
     1141 ccaatgcttt ctgttatatt ccaaatttgg ggattagttg ctacaactta tggagtgagt
     1201 tggattgttc aacctatagc ttattatcca acactaggag ctccaggttc ttatataggt
     1261 tggcttgcag gttctgttgg agatatacgt atgcctgcag catcaatggc acagaaaatt
     1321 gcaggtgtag aagcaggaac tcatgagggt gaagttgtag gaacaattgg tacatgttgt
     1381 tcaatctttg tatcagcttc tatgattact atttttacca ttgtaggcta taaagtaatt
     1441 cctttgttac ctgaatttat tactgattca tttaattata tacttccagc attgtttggg
     1501 gcaatttacg ttgatattgc gcgtaaagat attcgagctg gtggatgtac gattgctgta
     1561 gcattagcta ttatgtactt tggtgaaaaa attggtatac caggtggatt gttgacattg
     1621 tttattgtgt taggtgggat acttattaca agggttttct ttgtaattga tagcaataaa
     1681 acgaaagaat aggattgttt actaggaaga aagactagaa ttatttgctg agaaaaagaa
     1741 taggattgtt tactaggagg aaagactaga attatttgct gagaaaaaga ataggattgt
     1801 ttactaggag gaaagactag aattatttgt tgagaaaaaa gaataggatt gtttattaag
     1861 aaatatgaat ataaataagt cttgaaagga gaagaagaat gtttgatgtt aaaattgcca
     1921 atggaataat cattgatggt acaggaaatt caagatataa aggtgatgta gggattgtag
     1981 gagacaaaat catggctatt ggagatctaa gtcaaaaaga ggctaaagag actatagacg
     2041 caactggaaa atttgtatca cctggtttta tagatttcca cactcattct gatttatctc
     2101 ttgtgtatga caaatacaca agaagccgta tccatacagg tgtaacaaca gacgttattg
     2161 caaactgtgg tataggagtt gctccgataa gagaagagaa gaagcaggag ttaattgact
     2221 atcttggaac gaggataatt ggtacaattc caataaaatt gaaccttcac tggaatacaa
     2281 tgcaggaata ctttgattac ttaactgaaa attctccagc agtaaatgtt gtagcttatg
     2341 tagcacaagg acctgtacgt atcaatgaaa tgggattttc aaaggagcca gctactttag
     2401 agcaacttaa aaatatgaag ttggaagtta gaaaggctat ggaagctgga tgtgtagctc
     2461 tttcgtcagg attagtttat ttaccaggag catatacaaa gaaagaagaa atggctgaat
     2521 tatgtaagga attgatacca tacaatggat actatatatc tcatattaga gatgagggtg
     2581 atgaagaaat ggaagctctt gatgaagcaa tttatatagc taagacggca ggagttcctc
     2641 tacatatttc tcatttaaaa gtcatgggtc ataagaattt tggaactatt gataaggtat
     2701 ttaaaaagct agatgaagct gaggcagatg gcttggaagt tacttttgat tgttacccat
     2761 atacagctgg aatgacatct ttgggtgcac ttttacctcc ttgggctttt gaaggtggtg
     2821 tagaaaacat ggtaaaacga ttagaagtgc aagaaaatag ggacagaatt ataaaagagc
     2881 tagaggaagg gattcctgga tggcaatgtt tctaccagtt agcaggtggt tgggacggtg
     2941 ttgtactagc ctctgtaatg actgaagcaa ataaatatgt agagggtaaa actttaatgg
     3001 aggtagctaa aatcaatggt gaaaatccat ttgatacgtt ctttaggctt ttgattgaag
     3061 aaaaaagtaa aattcaagta gttgttcata cacaaggtca agaggataca gataaggtcg
     3121 tttgccatcc aaagtcatgt ataggttctg atagtatgga cctttctaca gaaggcttac
     3181 tttctcttgg aaagcctcat ccccgtgcat ttggtacttt tggacgaatt ttctcttact
     3241 atgttagaga aaaaggtatg ttgacttttg aggaagcagt taaaaagata acttatcttt
     3301 cagcaaaacg cttaggaatt tataaagaaa ggggtctact taaggaaaat tattttgctg
     3361 acattgtggt atttgaccca gatacaatag aagataaagc gacttatagt aatcctaaac
     3421 agtatacagt gggagtggaa tatgtattgg taaatggtaa aattgcattg gcaggaggaa
     3481 agcaaactga tgtatgtgct ggacgtgtaa ttaaaaatcc tcttagcata gcaaaataga
     3541 atacattaag aacttttata aaaatagtct ttctttgaat attaaggaag gctatttttg
     3601 ttaaattaat aaagattact ctacttgtgt tttctagaga atttaacttt aagtagaaaa
     3661 aacaatatat taattgtaaa atgtaattaa tattaatgga taagatttaa atacaaaaac
     3721 ttaaatacaa aaatgtttta acagatagtc ataattaaaa aacttaaagt aactttttat
     3781 ataagatata attgattatg caaaatttta tgaagcagca accttgttac tttaagtcaa
     3841 gggaggttca gaaagtgtct taagagataa tagtatcttt aggaaaggag aaacgaaatg
     3901 aaaatacttt atgtaggacc aggcatggta atcgacaaaa tactagcagt aagtaaaaag
     3961 aattttccca atattgaagc agattttctt agatatgata gatatattga agtacctgat
     4021 ttattggaaa aatatagagg tagagtagat gcagttcttt ttacaggtaa atcgccattt
     4081 aaattttttg aaaaaagaca aaaagatttt gtgttgtctg attatattcc tagacatgaa
     4141 acaacattat acagagtatt gcttgaaatt acatatttgc ttaaattcga tattagtaga
     4201 cttagtatag atacttatga taaagctatg ctgaagagat tgtataaaga aattggagtt
     4261 gaatttaagg atgaacatat gttttttgca gagcaaaagt atttagaaga ggactacata
     4321 aagtttgtgc ttgaatttca taaatataat tatcaaaata atgacatatg ttgttgtatt
     4381 acaggtcttg aagaagcata tcatgcaatg aaaaaagaga atattccagt agttttagcc
     4441 ttgccttctg aggatgtaat ggtacaaagt ataaaaaaca ttcagatgcg ttatattgca
     4501 aagaaaaaca atgagaatca gattgttgtt cttgctatca aacttaatat gccttcagaa
     4561 tattcactga tgaaggaaga tgagtacgct tatctttcac aaaggattaa gattcttgat
     4621 aaactatacc atttcaatag ccgtatagat ggtgttttgg tagagcagag tagaagcgaa
     4681 tttatgattt ttacaactaa aaaaatactt gagttagaaa caaagaagta cagagatatt
     4741 tatttgttgg atatgattcg tgaagtttca gttataaaca cttatatagg aattggttat
     4801 ggaaaaacag caaatgaagc taaatttaac gcttatgaga gtattaagat ggctcaaagt
     4861 caaaaaaatg atgcagcata tgtagttttt gaaaatggag aaattatggg accactaaag
     4921 ccaaatcctg ctgtcaaaga gaaaaatagc tttgatgaac gattttatca aattgctaca
     4981 gagacagggc taagtgctaa tactgtatat aaagtgttca ataaaataat aaatagtgga
     5041 aaaactgaat ttacatcaaa ggaattagct attatatgtg gtgtaagtat tagaactatg
     5101 gacaggatta tacttagatt gtgtgattct ggttattgtg aggtagtgag tgaacagctt
     5161 atgagcaagt atggtagacc cagtagaata ttacgtttta aaccttttct gttgttttaa
     5221 atagatataa gttaaaaatt aaatttttat gataacattt tctttacaat tttttagata
     5281 tatgttaata tttagaaaaa tgcatatgta ttcataaaat aaactcaaaa tagctaaaaa
     5341 atcattaaaa aggaaatcgt atgcataaaa tgagaaagtt aatttataac ttaaataaat
     5401 ctgggaggag ataattatgg gaaaatatca ggattcaaaa aaagttgtaa gaaattattt
     5461 tgaagcatta gaaaatgcta ctggggatga ggttgaaaat gtcttaaagc aacatatgaa
     5521 atcagattat aactggaaag gtgtgtatcc atttcgtgaa caagaaggca ctgaaaatgt
     5581 agcagatgta ttttggaagc ctttaaaaaa atcattgagt aatatgcaaa gacgacaaga
     5641 tattttcatt gcaggaacaa atgagattga caatagtgaa tgggtaatga gtatggggca
     5701 ttttatggga ctttttgata aggattggct tggcattaaa catactcgta aaatgattag
     5761 tttaagatat gcagaattta actgtgttca agatggtaaa attgctaaaa ctggattatt
     5821 tgtagatata attgggttta tgattcaagc aggagttaat ccattgccac cacaaacagg
     5881 ttcatatttt gtatatccag ggccaattga ccataatgga ttactttttg aagatgcaga
     5941 tgaagttgaa ggagtaaaaa ctttagcatt agtaaataaa atggtagatg atttatctga
     6001 attaaatgaa agtggtacta tgggatgccc accagaaata cttaaaaaat cttgggctaa
     6061 agatatgatt tggtatggtc caggtggaat tggtgcttct tatactatac ctagatatca
     6121 agagcaacac cagctaccat ttagaaataa tttaaaagga aagactttca atgggcatgt
     6181 atgtagattt gcagaaggta attttgcttg tttctttgga tggcctaatc tgactaatgt
     6241 accatgtgga ggttttttag gattaccagg tggagaggtt aaagcagata tgcaagttgt
     6301 tgatgtttat tatcgtaagg gcgataaact tcaagaaaac tgggtgttaa ttgatattcc
     6361 ttattggttg aaacaacaag ggcttgatat tcttgagaga actaaaagta tttttaatgc
     6421 ttagttatgc atacgtattc taaaacaaag ggggatataa agtggcatct accaacaaaa
     6481 ataatgtaac aagtaaagag gaagaaaaag aagtttctac aagtgggctt gaatcatgta
     6541 cggttaaaga aaacaaagct aaaatgaatc aaactaattt tagtaatata aatgaaaatc
     6601 aaacaaatga gaataaaacg aacacatata aaggaaatat agataaaata aacacaaata
     6661 aatccgacat aaataaagtt aacatagata cagtggatac agataaatta aatacaaata
     6721 ctcttactga aaaaaataaa aaagttatat ttaaggggga agataatatg tcatctacaa
     6781 aaatttacta tggagattca ttagaagtaa cgcctgttgg agtaatggat tacaatgatt
     6841 tttcaaaaca gacaaaaagg gaacaggaaa tacctggatt tgattctaag tatagagatt
     6901 ttgtagatta tattatgaag attacacata atatctggga agaaaaaggt ataggtgtta
     6961 tttatgatac atatcacaat aatgttacaa tgcattgtgg ctcttcaaat ttagttggta
     7021 ttaaagatgt tatttccaat actcttcaaa cattacatgc gtttcctgat agaagattaa
     7081 ttggtcaaaa tgtaatatgg tctaactttg gtgctgatgg ttttttatct tctcaccgtg
     7141 ttttgtctac agcaactaac ttaggtgata gtaattttgg accagcaaca ggtagaaaaa
     7201 taaactttag aactgtaatt gattgtgcca caactaacaa tagaatccac gaagaatggt
     7261 tggttagaga taatttatgg attgttactc aattaggatt aaaccctcag gaagtagcaa
     7321 agagtatggc aaaggcaagt gaatctaaag tgcttagctt acaatcaact tatggtatct
     7381 gtgaatctat ggatggtcaa tttatgccaa ctaagtatca agcaaaagat gattctgttg
     7441 gcgaaatgat gcttgaaatg acaagccgta tttataatta taaatatata aatgaagtta
     7501 aaaaatacta tcatgataat gcagtagtac attttatatg tgataaagat ttaaatggtt
     7561 atgatgagat tcaaggaatg attgtaagtt tacttgcatc aattccaaat ggaagttatg
     7621 aagtggaaag agtaacttgt aatcaaagag aaaaaaatga aggttatgat gtatctataa
     7681 gatggagatt acgtggaata aatgaaggta taggattttt tggacagcct tctggaaagc
     7741 atatagaagt aatgggaatc aatcattatc atatacttca aggtaaagta aaggaagagt
     7801 ggataacttt tgatggtatg gatgtattaa aacaaatgta tatgggagta gaagaataaa
     7861 gctgtttaga gtataaataa ttttgaaatg tatataggga agtattgctt tataaaattt
     7921 aagtggggaa tgtatatgaa aaatagatgt ttaaactttt agcaaaaatt atgactaaat
     7981 ttaaaagggg gaattataaa aatatgaaaa caacggttga aatagaaaat gatatcatat
     8041 taggaaaaga ctattcaaac ctaaaaagag gaattgcatt tttttcaatt gctttaatat
     8101 acttttttta ttgctacaat tttatggtgg gtacttttat taaaccaaca atgatttatg
     8161 ctttagcaga tgggggattt ggcttttcat taaaacaaac agaagaaata tttgcagtta
     8221 tgtcttttgg tacaattcca ggtacattta tttttggggt gatttctaca aaaattggga
     8281 agaagaggac tttaattagc gttgctttac tgattggatt gacaacattt ataccaatgt
     8341 tatctcctac agatattatg ttatggaaaa tagcacgttt gtgtacagga gtagtgttgg
     8401 gaggcgtatt tggaacagct atgcctttag ttgcagatat gtttccaagt aaatatcgtg
     8461 gtaagttagc tgctatatta actgctttat tttcattagc aatgattttt ggtgggaaag
     8521 tatatggcgt tttaggtgat gcaaattggc aaatattgat gtatactgcg attataccac
     8581 ctatagttgg agctatctta gcaatcttct ttgtgccaga tgatttggag tatacaaagg
     8641 aattagttaa aaagggaaaa gaaactggtg aaaagattag ctatataagt atgtataagg
     8701 gaaaatactt atggattgga ttgggaacga ttcttttatc aggtgcaaat tttacagcat
     8761 attcagcatt ttcaaataat gcaactacat acctagtgac aggaatagga atgtcagcag
     8821 cagtggctgg gtctatatat agtttacaag gaattggtca attagtagga tatttattct
     8881 ggggttcaat tgcagataaa tttggaagaa aaataccttt tataggtatg gcattagcag
     8941 cagtatttgt ttttatatat acaaaattag gtggagataa tacaacaaca ttctatatga
     9001 tatcagtatt actaggtgtt gctgttggat attctggagc atggggagca tactacacag
     9061 agttgtttcc aagtaaatat cgttcgttgt catctggtat gtcatttaat ggaggaagga
     9121 ttatatctac gtttgcaata cctgctatag ctggtacagc ttctggtact ttaggtatga
     9181 tgactatctt ttacatatca atcgcagtct ttgtagcagg tgctattgta tggttattct
     9241 taccagagac gttaaataat aaaaaataaa atactaggaa tttattttgt tacttagaaa
     9301 ggggtggctt atgatttcat caattgatgt atgtattata ataggatatt tgctattaat
     9361 gttagttatt ggttattatt caggaaaaga taataaaaat caagaagatt atttcttggc
     9421 aggtaggtca atgccatgga ttccaattgc tttatcagtt gcagctacta tgattagtgc
     9481 aaatggcttt ataggtggtc ctggatgggc atatgtagat ggaatgtatc cagtaatgta
     9541 aaatgtgcaa aaaattcttt tgtagttagt gcatttgtta tgaatttagt ttactacttt
     9601 atgctattgg taggagtaat tttatttgtt ttttatgggg gaaggacatt tgaaacttca
     9661 aatgaaataa tgattacttt tattttaaat gaacttccag taggagccat tggaattatt
     9721 attgcaggtg tgtttgcagc agcaatgtct agtatcgact ctatattaaa cagtatgaca
     9781 actgttttta cgaaggatat ttatgagttt tatttcaaaa aagacaacaa agaatcatca
     9841 ttaaaagtta caatgattat aactgtagtt ataggagttg ttatgacagg ttttattatt
     9901 atgggatttg gaggaagcat caaatcaatt cttgaccttg ttggtaacta tatctcatac
     9961 tttgctggac cagctacagg tgcatttgta ctagcaatgt ttacatataa agctaatgat
    10021 aaaggagtat ctattggttt tattgtaggg ttagttttag gattttttat ttctaataaa
    10081 tataatgtga gttggttgtg gaatccagca ataggtgcta caataacatt gatatttgga
    10141 tatattttta gtgtcatttt aaagaaaaat cataaagtag atgatattaa aaaatacact
    10201 gcatttggta tgagaaatca ccttatttct caaggaatgg ataaagtaga tggagtatct
    10261 atagtgccat tttctttagg taaacaagaa ctaattgtat tagctttttt tattatccaa
    10321 tatattatat tatttttgat acaattttaa gaagaaatac tagatattta aggagaaata
    10381 ctaaatgaat caaaagaaag ttcattataa taaatcggct acatcaaaag atgtcgcaag
    10441 attggcagga gtatctcaat catctgtttc aagagcattt ggtagtgcaa atggaaaggg
    10501 cgtaaaacct gaagtaagag aaagaatttt tcgtgtagca aacgaaatag ggtatgttcc
    10561 taatttggta gcacggggaa tgattagtgg aaaaactaat gttataggtt tgatagttgg
    10621 tgatagtcta ggtccatttt acaatagaat tatcaattta tttgtagaaa aaattcaaga
    10681 gataggtaaa cagtgtctcg tttttaaagt tcctagacaa caaagtatag atagtataat
    10741 ttcaaaagta attcagtttc aagtagaagc tgtgattatt acagcttcgg ccatgaataa
    10801 ggtgatggct gagacttgtg aagaaaacaa tattcctgtg attcttttta atcgttttat
    10861 acctggtata aaaataagta cagtctatgt agacccaatt gaaggtgggg gcatggttgc
    10921 tgaatatctg cttaagaaag gtcataagaa tataggatat attcaattta cgagagaaac
    10981 aagtgaagaa atggaaaaga aaattggatt ttattctaag ttaagacaaa gtgggattca
    11041 taaccttaaa gaagaatctg caaactatga ttatgattct ggatatgaag caggtaagag
    11101 aatgttagca ttatcaaatc ctccaacagc aattttttgc acaagtgact tgatagctat
    11161 tggtgtaatg gatgctgcaa ggtttgagta caagttgagg gtgccagagg atttatcagt
    11221 gataggatat gatgatattc aaatggcttc atggaagagt tataatttaa cgacgattag
    11281 acaaccacta gatttgttga ttgaaaaaac aattaacata cttaagtcct tattaaatga
    11341 agaaaatcca gagtcagtcg ttgaaatgtt aaaacctgaa ttgattgaaa gaggttctac
    11401 actttaagtg
//
LOCUS       DACTGW010000124        11392 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.124, whole genome shotgun sequence.
ACCESSION   DACTGW010000124 DACTGW010000000
VERSION     DACTGW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11392
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.124"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            71..1246
                     /locus_tag="I9573_002423"
     CDS             71..1246
                     /locus_tag="I9573_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT4797034.1"
                     /translation="MNLEKIKKFFGFPLDKKHKENVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDETDAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIEKAITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(1419..2816)
                     /gene="asnS"
                     /locus_tag="I9573_002424"
     CDS             complement(1419..2816)
                     /gene="asnS"
                     /locus_tag="I9573_002424"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAT4797035.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(3295..3489)
                     /locus_tag="I9573_002425"
     CDS             complement(3295..3489)
                     /locus_tag="I9573_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HAT4797036.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(3763..5436)
                     /locus_tag="I9573_002426"
     CDS             complement(3763..5436)
                     /locus_tag="I9573_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HAT4797037.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPVDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSQLNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     ISGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(5446..8844)
                     /locus_tag="I9573_002427"
     CDS             complement(5446..8844)
                     /locus_tag="I9573_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HAT4797038.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVVTVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIISFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSSINQDLETEDIGYRSINKSFKDEENRYKFIDGYNIQI
                     HNDLENEIYISKNASRQSGILSGIDVVHTPEFEEELAGLGMSQNFFKISDSLGVLSIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICSGLGNVNSEYAGIAEDSELIIIKLGKIDGFYNS
                     AMLFAASQYAYKKAFELRRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPHVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITVGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(9010..10206)
                     /locus_tag="I9573_002428"
     CDS             complement(9010..10206)
                     /locus_tag="I9573_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAT4797039.1"
                     /translation="MFIEFFYLLRSRGLNISLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMLSR"
     gene            complement(10227..11048)
                     /locus_tag="I9573_002429"
     CDS             complement(10227..11048)
                     /locus_tag="I9573_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAT4797040.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         3650 a         2066 c         1315 g         4361 t
ORIGIN      
        1 gaagtattcc aaatatctaa tgatagaaag gaatacttat ttttatgctt tatttttaat
       61 ggaggcttat atgaacttag aaaaaataaa aaaatttttt ggatttcctc tagacaaaaa
      121 acacaaagaa aatgttgaaa aagaactctg caattatctt tatcatacgg gtaaaatcat
      181 ttgcataata atagtaatat ctcaaataat tatgatgata tcaatctcag ctagagaagg
      241 aggccctttt gccacaccaa gacgccaatg gtatttttat atgtatgtaa cactattttg
      301 tgtatcttct gtctttatgt ttgtattttc ttatctttgg aataaaaaaa aggattgttg
      361 tacgttttat ataaatttat ggataattta tgtgactttt ttctgcctat ggggttgtgt
      421 aattactcta cttgaccagt taggtggaaa tgacctaagt gtatttagtt atattatgtt
      481 agcagctgca gctttctcag tattaaaatt tggagaatca gtaatagttt ttggaggatg
      541 ctttttgttt cttaatttat tcttacctgg gattcaaact ataccaggca atgagtttag
      601 taatattgtc aacacatttt ttataacatc ccttgccatt tttgtttcta caatgctata
      661 tagaaatcgt gtatttactt actatgataa acttttaatt agtcaacaat atgaagaaat
      721 atctcatata aataatttac ttaacgacaa agttatgaca gacgacctta ctaaaatgaa
      781 caaccgtaga taccttgaag atattctaca aaaaaaatta atcagccatg caaatcaaga
      841 acctataact ggaatgatga tagatataga ttatttcaaa caatttaatg ataattatgg
      901 acatcaaaag ggagactatt gtctacaaaa tattgccaaa gtaattttag attttacaag
      961 ttctgaaaat gaacatgcta tacgatatgg tggtgaggaa ttttttatat gtatttttaa
     1021 ctgtgatgag acagatgcta aacttaaatc agaatgttta cgaaaaatta tagagtcctg
     1081 caattttgag cgtgatgatg ttcctttaaa atgtgtaact gttagtattg gagtttatac
     1141 aaagaaaatt gaaaaggcaa taacattaaa tgagtttata agtaattgtg ataaggctct
     1201 atatactgca aaagaacagg gacgtaattg tgttgcattt tattaaaaag ttacatacta
     1261 ttatataaac ctataagtat tatatcgcaa aataaaagta atcaaattat atatttatag
     1321 tacaataaaa ataacttgca tatctctaaa atagataaat atgcaagtca ttttagtgtt
     1381 tatacttaaa gttatataag ttttattata tataattatt aaaattcagc atttttagga
     1441 gttcttggga aaggtataac gtctcttatg ttagacatac ctgtcatata cataattatt
     1501 ctttcaaatc caagaccaaa tccagagtga gtagcagtac caaactttct aagttctaaa
     1561 taccaccaat aatcttcttc tcttaatccc atttcattaa ttctatctaa caatacatct
     1621 tctctttctt ctctttgaga acctcctacg atttctccaa ctccaggaac taataaatcc
     1681 attgctctaa cagttttacc gtcttcattc attctcatat agaaagcttt tatatctttt
     1741 ggataatctg taacaaacac tggacaatta tatatttgct ccgttatata tctttcatgc
     1801 tcagtttgaa ggtcacatcc ccactcaaca ggatactcaa attcatgacc agattcaatt
     1861 aataattcca ctgctttagt atacgttatt ctaataaact cagagtttac tatcttattt
     1921 aatctttcta acaatccttt atctataaaa ctattaaaaa attccatttc ttcaggagca
     1981 ttttctaata catagcttat aacatatttt atcatatctt ctgcaagttc catgttatct
     2041 tcaaggtctg caaaaactat ttctggctct atcatccaaa attctgaagc atgtcttcct
     2101 gtataagagt tttcagctct aaatgtaggt ccaaaagtat aaacgtttct aaaagaaagt
     2161 gccattatct cggcatttag ttgaccacta actgttaaat tagcctcttt tccaaagaaa
     2221 tcttgagtat agtctatttt accatcttca gtttttggaa tatcatttaa atccatagtt
     2281 gttattctaa acatttctcc tgcaccttca cagtcactac ccgttatgat tggagaatgt
     2341 gcatatacaa agtttctttc ttggaagaat ttgtgtattg cataggcagc tattgatctt
     2401 actctaaata cagctgaaaa agtattactt cttggtctta aatgtgctat cgttcttaaa
     2461 tattcaagcg tatgtctctt cttttgtagt ggatacgaac tatctgattc cccttctaca
     2521 actatgcttt ttgcatgtat ttcgactggt tgtttagccc cttctgtaga tactaactct
     2581 ccctctacaa gtatagatga gcttattgct aattttccta cctctttaaa attttctact
     2641 ttctcatcaa atactatctg catattttta aagaagcttc catcatttag ctctataaat
     2701 ccaaaatttt tagatactct agatgttctt atccatccag ctacttttac agtcttccct
     2761 atgtactgtt ctttttctct gtacaattct tttactgtta taaattcctt ttgcatttta
     2821 aatcctccta gtataattgt tattaatttc ttttaaatat aatttcaata aaaaaagccc
     2881 ttcatcccta tataaaggga cgaaagacta ttactctcgc ggtaccaccc tagttgtcat
     2941 aatgacctct tagttaagca atgcttaaag cctataacgg tgccttccgt ctaagtctac
     3001 ttactaaata agctttcgat tagaaactcc aagatgttct tcaatataaa cctcgtattg
     3061 agctttcacc atcctcaact cgctagaact acattttata tctactcttc tcttcacagt
     3121 atttatcttt tattatttct actaattaac aaaattatac caatttataa atttttagtc
     3181 aataaattta tataattgat actgattgtt atttaaaata taataattct accttgcata
     3241 atgataataa atacttatct tcattacttc tttattactt aaatttccaa aattttataa
     3301 tttacttcca ttgtcaaccc agtcttttgc ttcttcgaca cccttaattg aaggtattga
     3361 aactttacta tgaggtttta atttttcaac atcagcccat gctgctgtcg actcatttcc
     3421 aactaaaagt gaacttttaa gtcttttatt atctttacct tttaatgctc ttgccttttt
     3481 actttccaaa actatcacct ctattattta ttttgtaata gttttagcaa ttttacaaat
     3541 aaaattcata agtctaaatt ataatgattt ttaaattaac atcatatctt ttcaatactt
     3601 aatttatacc tatttatata caaaatattt actttgtgta ttcaaaatat aaaactgttt
     3661 ttttaatttt catatttatt ttttatgcca tttttctact ctatctaaag tatttgatac
     3721 aattaaatta ctaatcaatt ataattttac ataggttctt atctatagag tatttgctat
     3781 ctgttgaatc gtatttttta aatttaaaat cccatatccc tgcgatacat ttggatatgt
     3841 atatatctca agttttgtag cacccaatat taaatatgtt tttaaaactt gtgtaaacaa
     3901 tgataatctc ggtacattat cctgtttttc taagtattcc atcaataagg ctaaaacacc
     3961 tgtgactata gaactgctca ctcctgttcc tgttcctgta ttatatgttc catttttata
     4021 tgtggatatt atatctacac ctgaagctac tatatctggc ttaattcctc ttcctctaat
     4081 tggtcctttc gaagaaccaa tccacatact atcagtctta ttattaaatg taccaactgt
     4141 aattacatca tcactagcag catacatagt tatagtagct actgaatcag ggtctaaaaa
     4201 ccttgtgtct ggagctatca aatttttatt tggtaagtat atgtcatatt ctccacttat
     4261 tataagttct gaagtcaatc ttaaaatcca tactccaggt tttatatctc ttaatcttat
     4321 ctccaaattt tcttttccag aggtaatata tggataaata aatctcattg cataagtagt
     4381 attttctaag ttaaattttc ctctatatat ataaaagtct ggagaatacc ttatatcatg
     4441 actaacttca cctgaaggag atattatctg tgctcccact ttatctggac cattcgtatt
     4501 tagtgttata tcaagagcat agtcatctcc atcttgtatt attacatctt gaacttctcc
     4561 cacactgcta aatctgccag aataatgaat atctgtattc ccttgatttc ctgcaccact
     4621 cactactacc acaccagcac tactcaatat attaaaagta tctaatattg aagtagttgc
     4681 tacagcactt gacttaactc caatagtcaa atttattatt agtggtttat tttctgttct
     4741 tgctatatta gtaacatacg ttatagctgc taaaaaatct gaaacactat aatttatcct
     4801 tccagcataa taagtatctg tatatgattt taatttaact actattaagt ctgattctgt
     4861 agtaatacct ctatactgcg aatttatcct tccatttcca gctaatatac cagaaactaa
     4921 agttccagta cctatattat cttgactcaa acttccatca tttctattta tggctatatt
     4981 taactgactt cttgtaaatt cacttccaaa aataaaacct tctggaggtg gatttgtatt
     5041 tgcttcttgg tcccataaat atagaacttt actagttcca tcatcattta taaaatcagg
     5101 atgcaaataa tctataccag aatctataac tgctaataaa attcctcttc ctgttatatc
     5161 attatatgga ttttcataaa tataatcagt ttcagctgct gttgtaatgg tttctccatt
     5221 atttacatta ttagttattt caattagact gctcattggt tcagattctt cccaccaagc
     5281 aacttgtatt atattattta aaatagtttc atcaaaatct acaggtacat atattactgc
     5341 aagctgacta tttaaaacca tatacctatt tattccattt tcttgtaggg cagattctat
     5401 atcaccttga taaattatac aataagattt ttccataaat ccctcctatc ttaaaacatc
     5461 aaacattcct ctaacatcca aaagcccata tccagaattt gtatttggat atactgtgtt
     5521 tgaatctttt ctagcaccag cttgcataaa agtttttatt ttttgcacat atgcttggtt
     5581 tggatatcta ccatcaacaa aagtatattg gaaatacatt gcagcagcac cagctgcatg
     5641 tgcacttgct gctgctgtac cagttatagt tgcatacgtg ttcccaggat aagcagctat
     5701 tatattgaca cctggtgcta ctatatctgg ttttaacctg tcttcaatag ttggtcctct
     5761 tgatgaactt tgccataaac taccattaat agtattgtag gctcctacag ttattaaatc
     5821 atcttgaata gcaggataat ttatagtgta aaaagggtct acctctctaa atctagttcc
     5881 actttttaaa agttctctat ttggaaggta taagttatat cttcctgtta taatataaac
     5941 ccctactaat cgaatcttcc atacacctct ttttgcattc tttaaagtaa cattagtaaa
     6001 ttgctgccca gaaaaagttg ttgggtatat atacgttata gaatattcag tcccctctaa
     6061 atcaaataaa cctgttactt tattataatt tgaaattcca acacttttac tttcttcacc
     6121 agtgggagat actattatta catcagcttt atctggtcta tttaaccaaa gttctaatga
     6181 taattcttcc tcgtcttcat ttaactctaa ttcaacttca acagaacctc caacatgtgg
     6241 tattattcca gatgtatgtg tctgtgtatt gccctcattt cctgctcctg ctgttataca
     6301 taaccctcta gtaaagaatg ctttttcact attgctccta ttggttaaac ctgccaagct
     6361 actggtacct aaagacatat ttataactag tggtcttcta agttcaaaag cttttttata
     6421 tgcatactga gatgcagcaa acaacattgc actattataa aaaccatcta tcttaccgag
     6481 ttttattatt attagctcag aatcttctgc tatacctgca tattcactat ttacatttcc
     6541 aagccctgag catataccac tcaacatagt tccttgacca acttcatcct gagatagact
     6601 agggtcattt tctgcgatag ctcgatttat atcttctcgt gtatattctg tcccaatata
     6661 aaaaccatca ggtggagttc cttcttttgt ttggtcccat aaatatacta tttttgaagt
     6721 accatctgga taaataaaat ctggatgtag atagtctatt cctgtatctg caattgatat
     6781 tagagtccct cttcctgtta tattaatatt aggattattt ttaaagaaat ttacgcccat
     6841 ttcttctgtt gcaactacac ctccaaaagt acctcgatta atttctccaa gtaatgtcat
     6901 ctttgtagtc gatacagttc ttatgattga aggaagttgc aaaactcgtt gtatactact
     6961 atagtctgta ttatttatag atagtacacc taatgaatca cttatcttaa aaaaattttg
     7021 actcatacct aacccagcta gttcttcttc aaattcggga gtatggacta catctattcc
     7081 tgatagtatt cctgattgtc tacttgcatt tttacttata taaatttcat tctctaagtc
     7141 attatgtatt tgtatattat aaccatctat aaacttatac ctattttcct catctttaaa
     7201 tgacttatta atacttctat atccaatatc ctccgtttct aaatcttgat ttatactaga
     7261 tagagtgtac aaatctatat ctgataaatt taaaaaacca aatcctgatg aattatttgg
     7321 ataagattga ttacttaatc tccttgctcc ctttaaaagt aatgccctta atttctgtga
     7381 atacaaaaat aaatcatttc catttacaat tccccattcc ataaacaatg aacaaacccc
     7441 tgtaacatga ggtgtagcca tgctagttcc agttaatgct ccacttgtac ctcctggtaa
     7501 aaaagatatt atatcttcac ctggagctaa taaatcaggt ttaaaaacac ctaattgtgt
     7561 atctccttca ccagaaaata tagatactat gtctgttctt gaattaaaac tacctactgt
     7621 tacaactcta cttgctgttc caggaactgt tacagtaagc tcttgagtag gaatcagaaa
     7681 tctagtattt ctatttagtc cttcagaagt tggcaaatat atatttacat taccagttac
     7741 tatatctatt ggttcaaata caattttcca taacccagga gttatctgtg tatttgaact
     7801 taattgtaga gtaactcttc ttgtaagaga gtacggtgct atagggtaaa aataaccagt
     7861 tattctagtt tcacccaaag tatttcttat ctcacctgaa gttagagaga ttgcttgtgt
     7921 ttgattgttt gatgggttta ccaaatgtac actaaaatca tcaacaaaat ctggccatat
     7981 atttatgttt aatatacgct ctccttcccc aactataaac tcaacttctt ctgtaatatt
     8041 attttgaagc ctaatccttt tatgacctcc tttatctgca ttatttccag ctgctacaac
     8101 tatattgttt ttccaaaata aacacatatc atctatatat tgttcaaata aagatgttcc
     8161 tctatgtgaa ccttcattac ttccataact tatatttaat gtaacaggca ttcttaattc
     8221 taatgcccta tctaaaataa atttaattgc cctcataaac tctgtacttc tcgaaaaaat
     8281 atcggtctgt atatttccaa ctctaaccac tattattctg gcatcacttg caatagttgc
     8341 acaaatacct gcaacatgtg tcccatgtaa actagttgtt gaaataggta tatacatact
     8401 tccatctatt gcattattta tatcttcatt agtatataat gttccttctc taaaaccttc
     8461 tggtggattt ccttgaatag attggtccca ataatataat atcttagacc tcccatcact
     8521 atctctaaac acaggtagag tataatctat tcccgaatca attataccaa ttatagttcc
     8581 ctttccagtt aaaccagttc tatttttaaa ccccgttatt cctgtacttg aaaaactttg
     8641 cacatcctga gtctgtaata taaaaggctt ttctataaat tcaatttctg gataagttaa
     8701 aagtgtattt acatcttctt cattacttga tgttattatt gcatatgatg aattaagtat
     8761 ctctactgat acacctaatt cttcttctaa tctcaatata tctccattgt attttacaat
     8821 taattcataa tttattataa tcacctcgac ttctaaaatt attattaatg tatatgtttt
     8881 gtgtaagtct aaaatgtatt agactatata attttttaag tttcagttat acaatttata
     8941 atagatatat taaaaaataa ataatgatgg tacaattttg aataaatcat accatcattt
     9001 atatttactt tatcttgaaa gcattaaatt tttcaatgct ttttcaagac cttttacagt
     9061 cagtggaaac atattaaatt cactttgtat cattctttga gtttggtacc aataattaat
     9121 tgaatcccat ccatctctaa gttctggatt catccataca attttactgt actttctctt
     9181 aaacctttta agccattcta taccaggtgt gtaattatat ggaccacgat agttaccacc
     9241 aatatgaagt aactcagaag gcgacataga agcatctcca acaattataa ctttatagtt
     9301 tttatctata ttttttaata tccattcagt attaatagat ttacttaacc aacattcagg
     9361 tgttgtaaat aatctatcat aaatacagtt atggaaataa tatatcttta catctttaaa
     9421 atgatttgat ttacttacag actgaaataa tgtattacat aaagtgctat accccctcat
     9481 tgaaccacct gaatccataa gcaataaaag cttcactgta ttttttcttg gcttctcaaa
     9541 aaataacttt aaatatcctg cattattaca agtctcttca atagtcttat ccaaatcaag
     9601 ctctgtcttt ggagcatcta ttctagtaga aaattgacga agtctcttta gtgcaacttg
     9661 aaattgacgc atatttaaaa cttcatcatc tctaaaatct ctgtattttc tttctcctgc
     9721 aacttcaaga acagctctac cataagtaga atttccacct atacgtatac cagtttgccc
     9781 ttttcctgaa tgtcctaact cggaagtgcc tcctgtgcct atccaataat taccaccatt
     9841 atgaggagag tcttgttctt ttattcgttc ctccaattta gctcttaatt tatcaagatt
     9901 tatatttgga ttttgttcca atctttcaaa ctcttctaaa tctatatctg gttttttaag
     9961 ccaattcata atctcttctg gaatttcttc ttggtgttct atacctttaa aatactccaa
    10021 aaacactgca tcaaacttat caaaatcact ttcacttttt attaaaatca tcctgcaaag
    10081 atagtaaaaa tttgaaaagt ttgaatagca cagcccttta tccaatgctt caattaaagt
    10141 catccattca tttaaagata tatttaatcc tcttgacctc aataaataaa aaaattcaat
    10201 aaacataatt atccagctcc tattaactat aagtgtcttt gcatagattc aatatcctca
    10261 ttattcttaa gtagtattcc tgcaaaagga acttcttttt ctattttttc aataggcatt
    10321 ccactcaaag ttaatgcttg tatccagtct attaactctg atgtactagg ctttttttgt
    10381 atgtccttta aacttctaat ccaataaaaa gtagtcatta cttgttccaa taggtgttct
    10441 tctactttat caaaatgtac tttaactatt tcttccatca tgtctctatc aggaaattct
    10501 atataatgaa atatacatct tcttaaaaaa gcatctggaa gttctttttc cgcatttgaa
    10561 gttataataa ctattggtct ttgtttagct cttacagttt cttttgtctc atttatgtaa
    10621 aactccattt tatccaattc ccaaagcaag tcatttggaa attctaaatc cgccttatct
    10681 atttcatcta ttaaaagaat tacttgttga tttgagctaa aggcctctcc tagtttacca
    10741 tattttatgt acttgctaat atcatctaca ccttctcctc caaattggct atcataaagt
    10801 ctttgaacag tatcatatac ataaagccct tcttgtgctt ttgtagttga ttttatattc
    10861 caaattacta aatctttttt taattcattt gaaattgctt gtgctagcat agttttacct
    10921 gttccaggtt cgcccttaat caaaagtggc ttttcaagag caatagcaac attaactgat
    10981 gccatcaatt ctggtgatac tacataatca ctacttcctc taaagttaga tatctctttt
    11041 tttatcataa atcctactcc ttttaaacag tattttctaa atgtatatag actttattac
    11101 tatttttgat ataaaaataa taatacaata catttgtata gtattattct aaaattctat
    11161 atctaaataa aatcacctct aaaatattta atttttatct ttatttgtca taattaaatg
    11221 aaatatatat tttaaagaat aataaaagat agcttattgt acaaaattta aattataaaa
    11281 acttagttta atcaaaggca ccactttttt tcattttaga ttatatataa acataatata
    11341 aataattttt attattttag ctataagcat aaagttttaa cgtatttata at
//
LOCUS       DACTGW010000125        11282 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.125, whole genome shotgun sequence.
ACCESSION   DACTGW010000125 DACTGW010000000
VERSION     DACTGW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.125"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            419..1030
                     /locus_tag="I9573_002430"
     CDS             419..1030
                     /locus_tag="I9573_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797041.1"
                     /translation="MNIKRINLLLSTVMAAVIISLPLAVTANAMEKTESDNNVKIELS
                     DKEVEELHNYWPEDKIFSDEKIDEILIKTGQFTQKELDKINQEVDDSAKKVNQNSRVK
                     YINGYNKYRTFTKNGKNYLYIYVSGKTLKKIKAGTDLASTFGGFLPKIWVELDVVAIG
                     KIITINMDGINTNYGIVLSYIEDKWNFQGATYTYRYDHWFYQT"
     gene            1058..1240
                     /locus_tag="I9573_002431"
     CDS             1058..1240
                     /locus_tag="I9573_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797042.1"
                     /translation="MKKRLYKVFLKTIPNLIIVILSLALSFYTNTFDIDLKILLLIPL
                     AYVLLFIYYYIKTKKD"
     gene            1480..2022
                     /locus_tag="I9573_002432"
     CDS             1480..2022
                     /locus_tag="I9573_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAT4797043.1"
                     /translation="MLIYLAILELEEDKIKFEKVYEKYRQIMFYVANKILKDDHLSED
                     AVHNAFLRIIKNIDKIDEVDSPRTKAFIVIIVERIAIDFYRKRKREKVSDIEEEYKNR
                     EINFSIEDKVCESNLAIALAKLNESYFQVLSLKFQYGFSNKEIANALNLGEENVYKRI
                     QRARKKLKEILEEMEVGNIE"
     gene            2054..2716
                     /locus_tag="I9573_002433"
     CDS             2054..2716
                     /locus_tag="I9573_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HAT4797044.1"
                     /translation="MLRESIPIATKIIIDDLLSDDELNHVFSKGFERKMKKLIKQQKR
                     SSLTNKIIFYSKQTAIVFLIVLASLLTITMSSEALRSRFFRLVIRVYEDLTSFVFSTN
                     GEDNLSLKNKEPEYIPKGFKEVDRVDDFVITYRNDENIEIQYVTDKISSNSIILDTEN
                     AKVKDIFVNGHKAKYIVKGTFLQLFWNDNNFIYLLNVDYQNSGESEFYEDILIKIAKS
                     IK"
     gene            2906..3223
                     /locus_tag="I9573_002434"
     CDS             2906..3223
                     /locus_tag="I9573_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797045.1"
                     /translation="MKSDLYLCKSNKLYRDNEVLEKFMEDLSFLIDDYKFSLETISIL
                     CKINIEKLTNFYAGKGYILYEEMSLIESVLAPFLSAIDIAKHNYDVVIKSRESDKPLI
                     FEN"
     gene            3245..3688
                     /locus_tag="I9573_002435"
     CDS             3245..3688
                     /locus_tag="I9573_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797046.1"
                     /translation="MKRLFALLLTLSLVILPSTLVFANEKAENDSIYAEAPLVFQSNE
                     KAYTHGSGGTAYIGYMSSKHLNWRITSSKGIVILFSGTVEIRNSKNMLVASYPISGGG
                     SSTISGQIDVKYLKKGDYKAILKGTSTTGGGVLVVKSGLVQMFTI"
     gene            3906..4349
                     /locus_tag="I9573_002436"
     CDS             3906..4349
                     /locus_tag="I9573_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797047.1"
                     /translation="MNLRKTVISGMCALVCSSAVAIPAFADEYQDKNIEQYSAVNYID
                     DTMVKMSYIKSGTCNLSISGSTAKVTCFVRGASNVTSTSIIARLQKSNDNGKTWSTIQ
                     TWNASGNISCSLSKSKTIGKGYYRVHSTIKANSESKNIISSTKKY"
     gene            complement(4480..4900)
                     /locus_tag="I9573_002437"
                     /pseudo
     CDS             complement(4480..4900)
                     /locus_tag="I9573_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4878..6083)
                     /locus_tag="I9573_002438"
     CDS             complement(4878..6083)
                     /locus_tag="I9573_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAT4797048.1"
                     /translation="MYKYYKGETDAISNYKMVTKRSNNKINTNFLKKFINEEVAYSLA
                     NKITYTSRLGDEKIINDLEYYTCHWSKKHDSDLLRYTLLFGFCYELYYVKNNEMKVRI
                     IKPTDGCHYENEDGETIYFFREFKKDFKDDIYIDVYDKEYIYHFDSNFKEIEKPTVNN
                     IFNGNVPISICKRSEELGKNTIFNDIKGLQDAYETNLSDISNEISDFRNAYLTFSECN
                     IKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTFVQNTLSSIKENIYKITSHINHNEK
                     MQSNTSSLAVVARLISTEWICSQNNDSIADTLFNRYKLLCIWLNKKYGFDYDYKDIKA
                     KFTPKIPHNDLVVANILSQLGDKLSTETGLSQPSFIDNPHAEMEKAKKEQEIVSEGEI
                     LLDESKDYN"
     gene            complement(6178..6720)
                     /locus_tag="I9573_002439"
     CDS             complement(6178..6720)
                     /locus_tag="I9573_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797049.1"
                     /translation="MLLCDPASAGGSKHDYSAFLVGSESENGLLCGRLAELAKINART
                     DFDKYILHMIYLLKVYPDITHVYIEKNTFNGTAANQLELKIKNDDVLYYRDIEIINEH
                     QKKNKDDKISTLIPVLNKGQMIFAEEDKAFIQQILNFTGQKYSLHDDAPDISAEFINR
                     IFNIKVNESITLLDRRNLGL"
     gene            complement(6760..6978)
                     /locus_tag="I9573_002440"
     CDS             complement(6760..6978)
                     /locus_tag="I9573_002440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797050.1"
                     /translation="MLFDFKNTNHLEDAKEFYWENEKEIKFPILWPSFWNCLDMALSY
                     YENPTSFKQEVQNDVNSIGEKWFKTIRT"
     gene            complement(7174..7917)
                     /locus_tag="I9573_002441"
     CDS             complement(7174..7917)
                     /locus_tag="I9573_002441"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797051.1"
                     /translation="MDNLERNIQLIFSYMAKTFIRNGATIREAEKEANEIIKTNSSNL
                     WGVNGLAYQLGKINLEFFCMYFIQDTYLPKEDNVAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGKKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKKFSDDVKYAA"
     gene            complement(8217..8774)
                     /locus_tag="I9573_002442"
     CDS             complement(8217..8774)
                     /locus_tag="I9573_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAT4797052.1"
                     /translation="MVVGYMRVSTAEQNEDRQLVTMKKHNVEKMYQEKISAKDTKRSK
                     LNAMLDFVREGDTIIVHDFSRLARSTKDLLEIVELLEKKHVKLISDKENLDTSTPTGK
                     LMLTMLGAIYEFERTNMLERQREGISIAKEQGKYKGRKEVKIDNFGSYYDRYKTRELN
                     KTQLAKELGVSRPTLDRLIKSHESN"
     gene            complement(8969..9157)
                     /locus_tag="I9573_002443"
     CDS             complement(8969..9157)
                     /locus_tag="I9573_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797053.1"
                     /translation="MNNKKKSFLISGTINVILLIIVFALFSYNIIENSTRNMIITVIL
                     IFIFEIIKIKILEKYYNL"
     gene            complement(9168..9743)
                     /locus_tag="I9573_002444"
     CDS             complement(9168..9743)
                     /locus_tag="I9573_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797054.1"
                     /translation="MLKKKIISFLMAGIFVLSSGYNLVYASTFEENEYKQELVNENEF
                     TIINSNENEMIVDKKGNNTYVSTKVTPGSWKNVKYLGQKSRKLSSPYTRERIIQTIQW
                     GLGYLSGGSSFLLGLAESIYREKVYDITKYGGVYTKTYYRRQITDIRNSHANVPVPYR
                     YEYVTYFYSSSNFKKVVYTERVGDGIKLANN"
     gene            complement(10076..11215)
                     /locus_tag="I9573_002445"
     CDS             complement(10076..11215)
                     /locus_tag="I9573_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HAT4797055.1"
                     /translation="MVIKLTIEEYKNKYSTGDKIILSLKSFDNKELYYHETDNILNSK
                     DEIDRLTSSKIFKIIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYNLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLDPLKIYNFMDELIIFMENSKVNDLVKSTIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLINKGYDTFKEFSISYIISKNRTKYYKAILDVENKGYNLTVFIEFMLKSIIQSINEM
                     RDMHDRKSLESILKEELFENDITLSATEEHILKYICSKDNYSMTLENYIKKNKGRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSREKDVYKVNEKYLNMLEIS"
BASE COUNT         3991 a         1535 c         1370 g         4386 t
ORIGIN      
        1 aaatggaaaa aatatcaaaa taaggctatg atttttaaag ccatagcctt accaataaat
       61 gtttaattat cactaaaaaa ataaattttt attcaaagaa tacacataaa tatttccaga
      121 ctcacttatt gtataagtta tattctattt tgtatgaagt caattaaaaa ctgaactata
      181 ttaaaattaa ttttataaat aacaaatata aataattaca aaaccttaac tgcgttaagt
      241 ttgaaatata ataatattaa aaacacttct ctcaaatcaa gtatcttttg ttatattttc
      301 ataagtttta aaaatataaa ttttttaaaa cttgtgtcca aaaaacaaaa ttaaatatgt
      361 tatatatgta aagggttaat tttaaaacct taaatttatt atagttaggg ggatttaaat
      421 gaatatcaaa agaattaatc tccttttatc aacagtgatg gcagctgtga taatatcttt
      481 accacttgct gttacagcaa atgcaatgga aaaaacagaa tcagataata atgtgaaaat
      541 agagttgtct gacaaagaag tagaggaatt acataattac tggcctgagg ataaaatttt
      601 ttcagatgaa aagattgatg agatcttaat taaaacaggt caatttactc aaaaggaatt
      661 ggataaaatc aatcaagaag tggacgattc tgctaagaaa gtaaatcaaa attctcgtgt
      721 gaaatatata aatgggtata acaaatacag aacttttact aaaaatggta aaaactattt
      781 atatatttat gtaagtggaa agacattaaa gaaaataaaa gctggaacag atcttgcatc
      841 tacttttgga ggttttttac caaaaatttg ggtagagctt gatgtagtgg caattggaaa
      901 gataataaca attaacatgg atggtataaa tactaattat gggattgttt tatcatatat
      961 agaggataag tggaattttc agggtgcaac atatacttat agatatgatc attggttcta
     1021 tcaaacttaa aagacatgct tttttaggag gaataatatg aaaaaaaggc tttataaggt
     1081 ttttttaaaa acaattccaa acctgataat tgtgatatta tcacttgcat tatcatttta
     1141 tactaataca tttgatattg atttaaaaat attgctttta atacctttag catatgtact
     1201 tttgtttata tactattaca tcaaaacaaa aaaagattaa tttttatgga attagatata
     1261 tacgaaaata attaatttaa aaatttaact ttgagaatca tttgtagagt taaggccctt
     1321 ataatcccga aaatggattt gaagggcctt tttactagat atctttaaaa agcttgttaa
     1381 gcatagttat attctattta agtagagtta attatatatt tatgttataa ttgaacttta
     1441 aaatattaat tcagaatttt tatattggag gaattctaaa tgttaattta tctagctata
     1501 cttgaactcg aagaagataa aataaagttt gaaaaggtat atgaaaagta tagacaaata
     1561 atgttttatg tagccaataa aatattaaag gatgatcatt tatcagaaga tgcagtacat
     1621 aatgcttttt taagaataat taaaaatata gataaaattg atgaggtaga cagtccaaga
     1681 acgaaggctt ttatcgttat aatagtagaa aggattgcta tagactttta cagaaagaga
     1741 aaaagagaaa aggtatctga tattgaagaa gagtataaaa acagggagat aaatttttct
     1801 atagaagata aagtatgtga aagtaattta gctatagcac ttgcaaaact taatgaaagt
     1861 tactttcaag tgctttcctt aaagttccag tatggttttt caaataaaga aatagcaaat
     1921 gcattaaatt taggggagga aaatgtatat aagagaattc agcgtgctag aaaaaaatta
     1981 aaagaaatat tagaagaaat ggaggttgga aatattgagt aataaaaaag atgattttga
     2041 tattacggat gatatgctta gagaaagtat cccaatagcg acaaaaatta ttattgatga
     2101 ccttctttca gatgatgaat taaatcatgt tttttcaaaa ggttttgagc gaaaaatgaa
     2161 aaaactcatt aagcaacaaa aaagaagtag tcttacaaat aaaatcattt tttattcaaa
     2221 acaaacagct attgtatttt taatagtttt agctagttta cttactatta ctatgagtag
     2281 tgaggcattg cgttcacgtt tctttagatt agtaataaga gtatatgaag acttaacttc
     2341 atttgttttt tcaactaatg gagaagataa tcttagttta aaaaataaag aacctgagta
     2401 tataccaaaa ggatttaaag aagttgatag agttgatgat tttgttatta catatagaaa
     2461 tgatgaaaat attgagatac aatatgtaac agataaaatt agttcaaatt ctattatatt
     2521 agatacagaa aatgcaaaag ttaaagatat atttgtaaat gggcataaag caaaatatat
     2581 agtaaaagga actttcttac agttattttg gaatgacaac aactttatat atttattaaa
     2641 tgtagattat caaaatagtg gagaatctga attttatgaa gatattttga taaaaatagc
     2701 gaaaagtata aaataatttt tttaagtgtc caagggtagc ctcttcattc gttatacaag
     2761 tatatatgaa aatgtgaggt gatttttatg gttttaaaat ctacatttac ctgagcgtta
     2821 aaaatattgt tataggaaaa attaatacat tatagttata acatgcttgc aataggaata
     2881 aaactttaaa attgaggtgt aaaaaatgaa atcagattta tatttatgta aaagtaataa
     2941 attgtataga gacaacgaag ttctagaaaa atttatggaa gatttaagtt ttcttataga
     3001 cgattataaa ttctcgttag aaacaatatc tattctatgc aaaataaata ttgagaaatt
     3061 aactaatttc tatgcaggaa aaggatatat actgtatgaa gaaatgtctc ttattgaaag
     3121 tgtacttgca ccttttttaa gtgcaattga tattgctaaa cataattatg atgttgtcat
     3181 taaatcgagg gaaagcgata aacctttgat attcgaaaat taatttaaaa ctgggggaat
     3241 acacatgaaa agattatttg cgttattact tactttaagt ttagttatat taccatcaac
     3301 tttagtattt gctaatgaaa aagctgaaaa tgattcaatt tatgctgagg cgccattagt
     3361 gtttcaaagt aatgaaaaag catatacaca tggtagtgga ggaactgcat atataggtta
     3421 tatgtcaagt aaacatctta attggaggat aacatcttca aaaggaatag ttatactatt
     3481 ttcaggaact gttgagatta gaaattcaaa aaatatgctt gtagcatcat atccaataag
     3541 tggtggagga tcttcaacta tatctggaca aatagatgtt aaatatctta aaaaagggga
     3601 ttataaagct atattgaaag gtacaagtac aacaggaggc ggagtcttag tagtaaaatc
     3661 tggtctagtt cagatgttta caatttaata ttataagtaa aataaaaatc atgaatttat
     3721 tgggggatag tcacaataca tattatttac agatattcat aactattata gttaaagaca
     3781 ttctattata aagaaaatgt ataatttaaa attataataa aaaaaattat cattagtgtc
     3841 caaaaatcac ttcttcattc gttatataag ttatataaat agaaaacaaa tgaagaggtg
     3901 attttatgaa tcttagaaaa acagttataa gtggtatgtg tgctttagta tgttcatcag
     3961 cagttgcaat acctgcattt gctgatgaat atcaagataa gaatattgaa caatattcag
     4021 cagtaaacta tatagatgat actatggtga aaatgtctta tattaagtct ggtacttgca
     4081 atttaagtat ttcaggaagt acagctaaag taacatgctt tgttagagga gcatcaaatg
     4141 ttacaagcac atcaattatt gctagattac agaaaagcaa tgataatggg aaaacatggt
     4201 caacaatcca aacatggaat gcttcaggaa atatttcatg ttctctttct aagtcaaaaa
     4261 ctataggaaa aggatattac agagttcatt ccacaataaa agctaactct gaaagtaaaa
     4321 atataataag tagtacaaag aaatattaaa aatagatatt gtatttatga gttgtagtaa
     4381 acaagaataa tagtttttta aaagcaaata taattaattt taaatagagt gccaatataa
     4441 aataggtgta taatatttat acttttttat attggcactc tattttataa aataagttat
     4501 tatagaaaat gaaacataat ttatatataa attttctgtt tgacctggta tataatcttt
     4561 tatatcacat atcagtattt tcccatcatt ataaccccaa ccaatcacag ttgcaggtat
     4621 catatcagct tctccatcat aaatgattgt atgtttatac atgatttttc cctcgctatt
     4681 atctatagtt ttattcaaaa taccttctgc tattaattta gcaactatgt ctttaatagt
     4741 ctgtatctgc tttactgtct acaaaacata cttcaattaa tatcgctgga gcttttgtat
     4801 gactaagcca atacaattcc cttacgtccg attttgcacc tctattttca gcagatttat
     4861 atatatcttc agtcaattta gttataatct tttgattcat ctaataaaat ctctccctct
     4921 gaaactattt cctgttcttt tttagccttt tccatttctg catgaggatt atcaataaaa
     4981 cttggttgag ataatccggt ttcagtagat aatttatctc caagttgact taatatattt
     5041 gctacaacta aatcattatg tggtatctta ggggtaaatt tagccttaat atctttataa
     5101 tcatagtcaa aaccatattt tttattaagc caaatgcaca ataatttgta cctattaaat
     5161 aaagtatctg caatactatc attattctgg ctacaaatcc attctgtact gattaatctt
     5221 gctacaactg ctaaacttga tgtattactt tgcatttttt cattgtgatt tatatgagat
     5281 gtaattttat atatgttttc ttttattgaa gatagtgtat tttgaacaaa tgtatcattc
     5341 atgtctttaa taagccattc tatttttcca tctccattaa cttgaagtat tccaagttct
     5401 ttcatccttg gcaaatcatc ttctttaata ttacattcag agaaggttaa ataagcattt
     5461 ctaaaatcac taatttcatt acttatatca cttaaattag tttcataagc atcttgtaat
     5521 ccttttatat cattgaatat tgtatttttt cctagttctt cacttctttt acaaattgat
     5581 attggaacat taccattaaa tatattattt actgtaggtt tttctatctc tttaaaattt
     5641 gaatcaaaat gatatatgta ttctttatca tatacatcta tataaatatc atctttaaaa
     5701 tcttttttaa attctctaaa aaaatagatg gtctctccat cttcattttc ataatggcag
     5761 ccatctgttg gttttattat tcttactttc atttcattgt tttttacata gtaaagttca
     5821 tagcaaaatc caaataatag tgtatatctc aataaatctg aatcgtgctt tttactccaa
     5881 tggcaagtat agtattctaa atcatttata atcttctcat cacctaatct acttgtatag
     5941 gtgattttat ttgcaagaga ataagcgacc tcttcattaa taaacttttt taagaaatta
     6001 gtgtttatct tattattaga cctcttggta accattttat agttactaat agcatctgtt
     6061 tcacctttat aatacttata catagtgttg tattcttgct tttttaaact ctagtcttca
     6121 tatatttctc ttaataaact taaatctatt tccatttaat gcatcacctc catacaatca
     6181 aagacctaaa tttcttctat ctaatagtgt aatactttca tttactttaa tattaaaaat
     6241 cctatttata aattctgctg atatatctgg tgcatcatca tgtagagagt acttttgtcc
     6301 tgtaaaatta aggatttgct gtataaatgc cttatcctct tctgcaaaaa tcatttgacc
     6361 tttatttaaa acaggtatta aagtagagat tttatcatct ttatttttct tttggtgctc
     6421 attaatgatt tctatatctc tgtagtaaag aacatcatca ttttttattt taagttctaa
     6481 ttgattagca gctgtaccat taaatgtatt tttttctata taaacatgtg ttatatctgg
     6541 atacactttt aataaataaa tcatatgcaa tatatattta tcaaaatcag ttctagcatt
     6601 tattttggct agttctgcca atctgccaca tagtaaacca ttttctgatt cacttccaac
     6661 aagaaaagca ctatagtcat gtttagaacc acctgcagat gctgggtcac ataataacat
     6721 tgtctttttg aatgtatgag tttctataca ttctctagat taagttctga ttgttttaaa
     6781 ccatttctca cctattgaat ttacatcatt ttgtacctct tgtttaaaag atgttgggtt
     6841 ttcgtaataa cttaaagcca tgtctaaaca attccaaaaa cttggccata atataggaaa
     6901 tttaatttct ttttcatttt cccagtaaaa ttctttagca tcttcaagat gatttgtgtt
     6961 tttgaaatca aataacaact ctttgaaatt aagccataaa ccagaattaa ataattcatc
     7021 tacattatca actaaaactc ctttttcaca tttaaatttc caagttggta aattctttag
     7081 cctactatag aaacattctt tatgttgtaa ggttcctaat gctataaaag ttgttcctct
     7141 ttttataatc ttaccatttc ttattactgg tttttaagca gcatatttta catcatcaga
     7201 aaatttttta aattttttct ctctagcatc ttctgttcta acattttctt ctgactgata
     7261 atcatcaagt atgataagat ctggacgatt attattgtat ttttttcctc tcattggtga
     7321 tgaagatgat attgcttcaa taaaggtttt attagttaat tctaattgag tactattgca
     7381 aatatatttt ctatcattat catttaagag aactccaaat gcttttttaa tatactcatt
     7441 ttcaagtaat gcatttttta tatcttttac gaatttttct gctgtagagc ctatatcaga
     7501 gcaaattaaa gtatatgtct tatgtttata acaatgtgac catacagttg ttgctaaatc
     7561 tccaaatgca cttttacctg ttcctcttgg ataaattctt cctaattgct gtgaaccatc
     7621 tccaattata gactcttgta tatcttccca taactcatga tgaactttag ctataggagc
     7681 agcaacatta tcttcttttg gtaaatatgt gtcttgtata aaatacatac agaaaaattc
     7741 taaatttatt tttccaagct gataagctag gccattaact ccccaaagat tgcttgaatt
     7801 agtttttata atctcatttg cttctttttc agcttctctt atagttgctc catttcttat
     7861 aaaggtcttt gccatataac taaatattaa ttgtatattt ctttctaaat tatccactcg
     7921 tatttacttc ctcttaattc ttctattttt ttaggttcag tgagtaatac aattaataaa
     7981 ataaacaatg caaatttttt tctaataaaa tcaccctctt tgaaatatgt ctctggtttt
     8041 taatttttgc tagaaaattt ctagaactca ttttctataa ataatacttt tttgaaatag
     8101 aaggataccc cctatactga aaaacagtta tttataatta ttttaaacat aattaaaggg
     8161 cataattcta tgaatttgcc ccactaaaat taacttaagc atatatattg ctcttcttag
     8221 ttactctcat ggctctttat taatctatct aatgttggtc tacttactcc caattcttta
     8281 gccaattgag tcttattaag ttctctagtt ttatatctat cataataact accaaagttg
     8341 tctatcttaa cttctttacg tcctttatat ttaccctgtt ctttagctat agagatacct
     8401 tctcgttgtc tttctaacat gtttgttctc tcaaattcat atattgcgcc aagcattgta
     8461 agcataagtt ttcctgtagg tgtacttgta tctaaatttt ctttatcact aattaacttt
     8521 acatgtttct tttctaagag ctcaactatt tctaataagt ctttagtact tctagcaagt
     8581 ctactaaagt catgtacaat tattgtatct ccttctctta caaaatctaa cattgcgttt
     8641 agttttgatc ttttagtatc tttggcactt attttctcct gatacatttt ttctacatta
     8701 tgctttttca ttgtaactaa ttgtctatct tcattttgtt ctgctgttga aactctcata
     8761 taacctacta ccatctttga tacctccacc tatgtttact ctataaataa tattatagca
     8821 taaatgtaaa actagagtta aagatttgcc atgctttttg taaattaatt ggagttttga
     8881 ttttattttt acatcagaat agatagtttg taatgtaaat ttgtaccagc atactttaca
     8941 atatgctctt aggaaagata tttcttgttt atagattata atatttctct agtatcttta
     9001 tttttataat ttcaaaaata aaaattaaaa ttacagttat tatcatattt cgtgtactat
     9061 tttctataat attataacta aacaatgcaa aaacaatgat taataaaatt acgtttatag
     9121 tgccagaaat caagaatgat tttttcttat tattcatata tttcctccta gttatttgct
     9181 agttttatac catctcctac tctttcagta tatacaactt ttttaaagtt agatgagcta
     9241 taaaaatatg taacatattc atatctgtat ggaacaggaa cattagcatg actgtttcta
     9301 atatcagtta tttgtctcct atagtatgtt tttgtatata cgcctccata tttagtaatg
     9361 tcatatactt tttctctata aatactctca gctaaaccta ataaaaaaga tgaccctcca
     9421 gaaagataac ctaatcccca ttgaatagtt tgtataattc tttctcttgt ataaggagaa
     9481 cttaatttgc gtgatttttg acctagatat ttaacatttt tccaactacc aggtgtaact
     9541 tttgttgaaa cataagtgtt attgcctttt ttatccacaa tcatttcatt ttcattgcta
     9601 ttaataatag taaattcgtt ttcattaaca agttcttgtt tatattcatt ttcttcaaaa
     9661 gtagatgcat aaaccaaatt atatccacta gataaaacaa atattcctgc cattaaaaaa
     9721 cttattattt ttttctttaa cataatattt tactaccttt acttaaatat ttattctata
     9781 aacactgtca tataacctgt tagttatatt aaaaataatc tttttatata acttcacaat
     9841 caaaccttgt cttatttcat aatagtttta tttaatatca tataatttag acctccttat
     9901 aagattattt atatatataa cgaataataa aagtaatttt ggacaaaata aaaatctttt
     9961 tattttaaga atgaaaaatt taaccctcta agaaaactca attaagagcg attcatagag
    10021 ggcttattat tatattatat aactttatat actttttatt tttatatgct tcaatttaac
    10081 ttatttcaag catattcaag tacttctcat taactttata tacatccttt tctctagata
    10141 aaatctctat ttcttcaaga ttattaaaca cttccatcaa ctggtcaact aattcgattt
    10201 cttttattcc agcctttaag tatctacctt tattcttttt gatatagttc tctaatgtca
    10261 tagaataatt atctttacta cagatatatt ttaatatatg ttcttctgtt gctgatagag
    10321 ttatatcatt ttcaaataat tcctctttta atatactctc taaagacttc ctatcatgca
    10381 tatcacgcat ttcattgata gattgtatta tagactttag catgaactct ataaatacag
    10441 ttaaattata tcctttattc tctacatcta gtatagcctt ataatactta gttctgtttt
    10501 tagatattat atatgatatt gaaaactctt taaaagtatc atatccttta ttaattaaat
    10561 atagatatga taaggctctt gatgttcttc cattaccatc actaaaagga tgaacatata
    10621 caaagtagaa atgaattatt gtactcttta ctaaatcatt tactttacta ttttccataa
    10681 aaattattaa ttcatccata aaattatata ttttcaaagg atcaagtcct gtatgtataa
    10741 tctcaccttt tgaatcacat acatatactt tttcagttct atattcatat tcttctgaat
    10801 ctaaacagtt ttcacccaat atatgatgta aattatatat aaatttgtga ctatataatt
    10861 tatctaaatt atctaatcca tattctaagg ctctatgatt attgtaaatc atatactcac
    10921 ttttattact tggtttcatc tttcctctaa ttatagattt tgcaatcttt ctagttgaaa
    10981 aggcaccttc tattacacta gaataataag actctaaggc taaatcattt ttatcttcta
    11041 ttattttaaa tatcttgctt gatgttaatc tatctatttc atcttttgaa tttaatatgt
    11101 tatcagtttc atgatagtat agttctttat tatcaaaact ctttaaggaa agtataattt
    11161 tatctccagt agagtattta tttttgtatt cttctattgt cagtttaatc accaccttag
    11221 tttatcttta tctattaata ttaacaaaaa gtattagttt ttacactatt tttgattgtt
    11281 ta
//
LOCUS       DACTGW010000126        11232 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.126, whole genome shotgun sequence.
ACCESSION   DACTGW010000126 DACTGW010000000
VERSION     DACTGW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.126"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            10..321
                     /locus_tag="I9573_002446"
     CDS             10..321
                     /locus_tag="I9573_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAT4797056.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      414..506
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            606..1400
                     /gene="thiD"
                     /locus_tag="I9573_002447"
     CDS             606..1400
                     /gene="thiD"
                     /locus_tag="I9573_002447"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAT4797057.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1424..2212
                     /gene="thiM"
                     /locus_tag="I9573_002448"
     CDS             1424..2212
                     /gene="thiM"
                     /locus_tag="I9573_002448"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAT4797058.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGIYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            2235..2867
                     /gene="thiE"
                     /locus_tag="I9573_002449"
     CDS             2235..2867
                     /gene="thiE"
                     /locus_tag="I9573_002449"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAT4797059.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITERYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEATCAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3006..3308
                     /locus_tag="I9573_002450"
     CDS             3006..3308
                     /locus_tag="I9573_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4797060.1"
                     /translation="MIKILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRVPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3374..4129)
                     /locus_tag="I9573_002451"
     CDS             complement(3374..4129)
                     /locus_tag="I9573_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797061.1"
                     /translation="MGMLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMN
                     NININNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQL
                     LEAILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLP
                     NEIVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETI
                     LIGDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4117..5079)
                     /locus_tag="I9573_002452"
     CDS             complement(4117..5079)
                     /locus_tag="I9573_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797062.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5083..5541)
                     /locus_tag="I9573_002453"
     CDS             complement(5083..5541)
                     /locus_tag="I9573_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HAT4797063.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5897..6253
                     /locus_tag="I9573_002454"
     CDS             5897..6253
                     /locus_tag="I9573_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4797064.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6325..7041
                     /locus_tag="I9573_002455"
     CDS             6325..7041
                     /locus_tag="I9573_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797065.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7034..7858
                     /locus_tag="I9573_002456"
     CDS             7034..7858
                     /locus_tag="I9573_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797066.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQNILFFGMLGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIVLITMIYGFLISQTATLFICKYIFNFIFRNIPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIINLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            7870..9069
                     /locus_tag="I9573_002457"
     CDS             7870..9069
                     /locus_tag="I9573_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797067.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQDIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            9078..9890
                     /locus_tag="I9573_002458"
     CDS             9078..9890
                     /locus_tag="I9573_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797068.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFIFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVLEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            9901..11169
                     /locus_tag="I9573_002459"
     CDS             9901..11169
                     /locus_tag="I9573_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797069.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNKNPKSRIRNDYLNIDKKYSDFVNDKG
                     KELIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     YQQNINEIKFSIGSKLYLEIVNNKSKNGDIEGHDSYSNLIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMIKNNKLNLIVTDMSNSIINLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGMKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT         4493 a         1162 c         1796 g         3781 t
ORIGIN      
        1 tgatttaagg tgtattttac atatattata agatgtaaag atgattctct atacactgga
       61 tataccagta atatagttag aagaatgaat gaacataagt taggaattaa ttcaaaatac
      121 acaagagcaa agggatttga aaaactagaa gtttattttg tgaccaacac aaaaagcaat
      181 gctatgaagt tagaatacta tataaaaaaa ttaactagaa ataaaaaatt gtctataata
      241 aaaaacccaa gtatacttat aaatttaatt gataataaag aagattatat tattggtaag
      301 gaaatagaac aattaacata aatatttata gtaacattat aatgggtgca tatagtgtaa
      361 aaacttgaca ataaatacta ttgtaatata atgaagttat aaatatagta aattaaaata
      421 gctaggggag ccaagagctg agaggtatac cgacccttac acctgatctg gataatacca
      481 gcgtaggaaa gcttagtaaa aattcaagta tgtattattt gtgatatatg ctatttctaa
      541 gctataccta ttgtaggtat agctttttta attaagttat ttaaattaaa ctttaggagg
      601 caacaatgag taattataaa ataccaacac ttacaatagc tggctctgat tcatctggag
      661 gagctgggat tcaggctgat ttgaaaacgt ttagtgcaat tggtacttat ggtatgagtg
      721 taataacagc tataacagca cagaatacta aaggagtttt tgctgtagag gatttaaata
      781 agaaaataat aaaaaaacaa atagaggcag tctttgaaga tatacctcca agagctgtaa
      841 aaataggaat ggtttcaagt cctgagatta tacttgaaat agttgagaat ctaaagaaat
      901 acaatccaaa atatttggtt gtggacccag taatgatatc taaaagtgga tattatctat
      961 taaagccaga ggcaaaagaa aacttaatta agtatttgat accacttgca tacataatta
     1021 caccaaatat accagaggca gaagaaataa caggaataaa aatacataat gtagatgata
     1081 tgaagagagt aggagaagaa atactacaac ttggacctaa atttgtactt atgaaaggag
     1141 gtcatttaga tggagaagca gtggatattt tagttggtaa aaatatattt aaagtttata
     1201 aaagtgaaag aattgataaa aaaaatacac atggaacagg gtgcacatta tcttctgcaa
     1261 taacatctta tctagcatta ggatatgaaa taacagaagc agtaaatcta agtaaaatct
     1321 atataacaga agcaataaaa agaagttttg atataggtca tggagtagga ccagtacatc
     1381 atttttataa atttgagtaa taaaaaaata ttggaggtag aatatgtata atttaattaa
     1441 agatgtaaaa aaattaaatc cattggtaat tcattatact aataatgtaa ctataaatga
     1501 ttgtgctaat gtgactttag ctgtaggagc aagtcctctt atgagttttt catatgagga
     1561 agtagaagaa atggtaagtg tagcaaattc tgtagtaata aatataggaa ctatgaactc
     1621 aaatatgttg gatttatttc tattagctgg aaaagcagca aataagtaca ataaacctgt
     1681 tgttttggac ccagttggag tatttgcaag caaagcgaga gcagaactta caagtagact
     1741 tttaaatgaa gtcaaattta gtgttgttaa agggaatgta tcagaaataa agtttatagg
     1801 tggctttaat gtaaggggta aaggtgtaga ttcatttgat gaagaagaag attctactga
     1861 gataataaga aagattgctg aaaagcttga atgtgtagtt gtagcaactg gaaaaataga
     1921 tataataact aatggtaaag gtatatataa aataaacaat ggaactgata aattaaaagg
     1981 aattacagga actggatgta tgactgctag tttgattgca agttttatgg ctgttactga
     2041 gaatatatta gaagcagcaa caatgggtgt tcttacaatg tctttaagtg gggaattagc
     2101 taatttaaat aatccaccaa taggtacatt taaagaaaat cttatgaatg ctatttatca
     2161 aatggatata gatactttaa gtaaaaattc taatattgaa tttttaaatt aataaagagt
     2221 aagggagact taaaatgatt gataaagaat ctttaaaaaa atgcttaaag ttgtatttgg
     2281 taactgactc tgaaatgctt aaaggtagag acttttataa atgtcttgaa gatgcaatat
     2341 cttctggaat aacaactgta caacttagag aaaaaaatgc ttctggaaga gagtttttaa
     2401 gaaaagctat gaaacttaga gaaataactg aaagatatgg tgttaagttt ataataaatg
     2461 atagagtgga tatagcactt atttgtgatg cagatggagt acatgtagga cagagtgaca
     2521 ttgatgtgag agaggttaga aagcttatag gaaacaataa aattctagga gtatcagcaa
     2581 gaactttaga agaagctact tgtgcaaaaa atgatggcgc agactattta ggtgtagggt
     2641 ctatattcac aacatctact aaattagatg caaaatcagc ttcgtttgaa actgttaaag
     2701 aaattaaaga aaaagtagac atgccatttg tgctaattgg aggtataaat ttagacaata
     2761 tagataaact taaatgttta gaaagtgacg ggtatgctat aatatcagct atattaaaag
     2821 ctgaagatat ctctaaagaa gtggaaaagt ggacattaaa aatataaaat tggctctggt
     2881 agtgaaaaaa atggaaaaat ttgatataat ctaagagtaa aatttaagca attataaatg
     2941 ataaactttt ctacatatac tttttaatca agatatatgt agatttggtt aaaaaggagc
     3001 tataaatgat taagatattg ttagtgtgtg ttggaggcat gtcttctacc ctattggtaa
     3061 ataaaatgga gaaggatgca aaaaaaagaa atatagactg taaaatatgg gcagttggag
     3121 aaggagatat aaaatcggag cttgataact ttgatatttt acttcttgga ccacaactta
     3181 gatttatgct tgatgacgta aagtctatag ttggagatag agttccagta tctataatag
     3241 atatggtaaa ctatggtact tgtaatggtc atgctgtgtt aaattcggtt ttagaaatat
     3301 taaaataaat aataaaagtg gattctatat aatagagtcc atttttatta tttgttatta
     3361 ataggcttaa aacttagctt accatttctt tttttaatct ccacatagca agtgaaaaac
     3421 ttgaacagca caatattaaa aatagtagga taacaggcag tatatctcca ataagaatag
     3481 tctcaccaaa tattaagcta cgtaaagcgt taataacatg ggtaaatgga ttgagcatca
     3541 ctgctacttt taatcctcca gataaacttt ctatagggaa aagagctgaa cttaaaaaga
     3601 aaataggaag tacaattgca gtcataattg tttcataaac tatttcattt ggcaataata
     3661 aacttataga ataagctaga cttgataaaa agaaggctgt cataaatatc agtactatca
     3721 taagtaggat tccaccaatg ccagattcta tccttactga gaagaatata cttactatac
     3781 acaaaatagt tacttctatg aaggaaacta agatagcttc taataattga ccaaggacga
     3841 ttgaatacct actgataggt gcaattaaga ctctatagaa acttccactg ttcttcatta
     3901 taaagtttat aataccacca ctactacaag aagaaaacac aaccagtaca atgatacctg
     3961 gtagtataaa agcagtataa ttattgatat ttatattatt catagtttgg tttgcaatag
     4021 aactataaag cacaagccaa agaaaaggtt gtaatatagt aactacaaat gagattggat
     4081 ttttaaaacg ccacttcata ctacgccata agatatttaa cattcccatt cctcctttaa
     4141 gcttttttta gaacttgtaa gtgctaaaaa tacatcttcg agacttggct ctacaatttc
     4201 tattgcatca aattctatat catgttctaa aagccattta tttattaaag taaaagcagt
     4261 tctactatcg tttacactta taaaaattga attttctcgt acgctcataa actttactaa
     4321 accagtactt ttaattgaat ctttatattt ttttatatct tcagtattat ggaaagttat
     4381 acgtagtata ttttgtctaa tgtaactacg taaacttgat ggtgttcctt gtgctaaatc
     4441 ctttccattc ttcataatac aaatattatc actcaattgt tcagcttctt caagataatg
     4501 agtagttaga aatatagttg tgccgtactc atctctaatt tttaataaca tatcccacat
     4561 agattttctt gaatcgacat ccataccaac agttggttca tctaaaaata ggattttagg
     4621 agatgatacc atattcatag ctatgtccaa tctacgtttt acaccccctg aatatgatga
     4681 agtaggatat tttaagtaac tagataagtc aaacttatca attaaagagt ctattctttg
     4741 ttttgcaatc tgtggttcta ctttatacat tttactttga aataccatat tttccataag
     4801 ggataaatgt tcatcaatag agatttgctg tgctacacaa gcaatttgag tacgtatcca
     4861 agaaggatta tctactaaat cttttccaaa catttttaca tttccagatg taggtttgta
     4921 aaaggtagtc aaaatattta taagagaaga tttaccagca ccattaggac caagtaaaga
     4981 aaaaatttct ccagcattta cattaaagct tagaccattt aaagctttca caccattttt
     5041 atattctttt actaaattgt ctatttcaat cgctaacata tattattcct cctctttaag
     5101 tggaaatatt atctctgtaa tatagttatc tgggtttcct tttatcaaca tacctggtcc
     5161 tttaataaat acttctctcc aaggagtttg tatttttata ttattttcat gaatatattt
     5221 aaatatcatt tcatacgttt ttggcaaatt actataagag cctgtgtgag taaaaaataa
     5281 agcttttgtt ttaggaaatt cttttgtagt tatacccata cgatttggag tttcagcagt
     5341 tggaacacat aattccattt ctgcaacacc agttttttgg tcaacatcat agtagcaaaa
     5401 gaatggtgca ccgttagact ttccttttat agctttaaat acatttggaa aatactttgt
     5461 tgcctcagtc ataggtccat tataacgcat ggtagccact gtgactgatt cgatattttt
     5521 gatttcaatt ttgcaattca tttgtttacc tcctaatgtg tgttcatatt cttactagat
     5581 ttttaagcat taatacatat aagattttga ttagttgaaa acatataatc ttatctcgtt
     5641 tgcttgtttt caagtctaac ataacataaa atatatatcc tctcatttga tgctccattt
     5701 tgtaaagcta ttatttaagc tttactttag attttttagc aaattgaaat aaagcaattt
     5761 ataaaataag tatttacaaa ttaagtgtat taatcgtata atacacttaa taggatacta
     5821 gataaagtct gaaataaagg cgtatttttt taaagtaagt gtactatcgt attaatacac
     5881 tagaatagga ggaatcatga attttgaatt agataataca actcccattt atttgcaaat
     5941 agtaaagtat ataaagagac aaatagtaac aggagagttg aaaccaggag agacaatacc
     6001 atcaagaaga gaaatggcac ttaatctaaa agtaaactta aatacagttc aaagagcata
     6061 taaggagatg ggtgatatga atataataaa tacttttaaa aattatcaaa gcagtgtaac
     6121 agttgatgaa aatatattaa aaaatttaaa attagagctt ataaatgaat cactatctgt
     6181 ttttatagaa gatatgaaag caataaatgt tagcaaagaa gaagttttaa aaataataga
     6241 agataagtat tagtttttta acaaatagaa aacttagttt agaaagttta tttataagca
     6301 tattggatgt ggaggatagt aaatatgata gaaataaaga atgtcagcaa aacatataaa
     6361 aggatgcaag ggcttaaaat aagaaagata gaagctttaa aaaatgtaag ttttaatata
     6421 gaaaagggaa aaataactgc attacttggt ataaatggtg ttggtaaatc aactatgtta
     6481 aaagcaatag caggacttat aaacattgat agtggagaaa ttcgtataga tggagaaaaa
     6541 ataaatgaaa aggtatacaa taaattggct tttgttccag atgtacaatc tcactttagc
     6601 aatacaacaa taaaagaaac atttgaattt atggaaattt tttattcaaa gtggaataaa
     6661 gaaaaatcaa aagaaatgat ggacatattt aaattggatg aagatgaaat aattgataac
     6721 ttgtcaaaag gaaacattgc aagagtaaaa ttaatacttg gattttgcca agacccagag
     6781 tacatacttc ttgatgaacc atttacagga attgatttgt ttaagaggga agagtttata
     6841 ggagtaatag cgcaatatat ggaggaaaat caagccatta tcataactac tcatgagata
     6901 gtagagatag aaagtttagt tgatgaggtt gtaattcttg atgaagggca gattataact
     6961 tcatttaatg cagaagaatt aagagaaaga gaaggtaaat ctatattaga taagatgagg
     7021 gaggtatata agaatgaata aacttctata tctaataaat gtagatttga gaagaagcaa
     7081 taagttttat atagcttata tatcattttt ttcattaatt acacttagtt taaatttatt
     7141 cgaaattaat aaatttaaaa attctaaatt tattatgaac ttagcaatag aagattttgg
     7201 aggtatattt tatggactag ggatacttag taatcttggt tttatccaaa atatattgtt
     7261 ctttggaatg ttgggacttt ttatatacta tatttttatg tggaaaagag agttcttttc
     7321 aaataatcaa agtatttata cacttatgat gttacctcaa aataagttta agatttgtat
     7381 atctaaatca atagttttga taacaatgat atatggtttt ttgataagtc agacagcaac
     7441 attatttata tgtaaatata tatttaattt catttttaga aatattccta taatcaatat
     7501 gaactttgcc aaagatttat attatttaaa cttgaagagt ataccaattg attttatctc
     7561 ttttatggct atttatgtat ttttactact tatagctatt tctattgtaa attgctgtat
     7621 attatttatc ttttcctata taaataaatt ttatgtaatt ttaatcactt tgctgattat
     7681 agcaggctta tatttttcaa caatatatat aaatataatc aatttattta gaaaatatta
     7741 taatcttata ggatttcaat ttaacaattt agtagcaggg ttattaagtt ttttaattat
     7801 aatgtttatt ctaaatggaa tatcttattt gctaattaaa aagaaagtat ctctttaagg
     7861 aggggtggta tgaaattaaa atctaaaata tctatactta ttatattttt gactttagca
     7921 atatatttta gtataggttt attaaataat gaagataatt taaagataaa aactataaaa
     7981 ggtgactcta atgaattaga ccatataaac ataaattttg gatatgcttt gaatggatat
     8041 ataaattcat attatacaat aaatggaagt aacaaaacta aaaagactga attaataaaa
     8101 ctacaaaagg atagtaagaa cacaaataat ttaattgatg tatctagtgg ttcagattta
     8161 attgctcatg ttggatttga aatgaatgag gagtcaaaag atataaactt tgtgatagat
     8221 gagaaaaaag aagatttaga aaaaggtgta tttccatcag aaggtagtga taaagaaatt
     8281 gaatatgaaa aattagatat gaataatttt acaaggacaa gatataaagt agataaaagt
     8341 acagatgatt attatgcaaa tatactagca tcaaaaagat ataaaaaata cttatatgta
     8401 tctgtacttt atgctacaag tgattcatca gaaaataaca aagaatattc aagtactata
     8461 gatataataa aaattaatcc agaaaataaa aaaatcaaga ctataaaaag tataaattta
     8521 agtaattatt tttccgagaa ataccaagac atagagaaca taggtgtaag ttatgctagc
     8581 aaatatgagg ataagatgta tattttagta aatgtactaa atacaaacat aggtgataat
     8641 agtaattcta aatttcattt acttgagtat aatcttgaag aaaataagga aagcataatg
     8701 gaattaaaaa ctgatagcaa aatggcttta gcaaaagcaa agttagaagc taataaatta
     8761 tatatggtaa tgtcgaatga ttatgataat agttcaatta cagagttaaa tttacttacc
     8821 tataatatat tagataaaag cttagatgaa gataaatatg ttttagataa tagagaaaaa
     8881 agaggaatta gaagtattga aattgattca aataaaatcg ttgcatatac atcaaggcta
     8941 aactctatat tatcttggga taataaattt atgctatata taataaataa aaattctaag
     9001 gaaattgtat atgaaggaga attacaattt aacagttcaa atagtatgtt agaatttgaa
     9061 ataaagtagg tgagttgatg aaagatttaa taagattatt cgaatttgaa ttaaaaagaa
     9121 atataaagaa ttatatattt ataataattt gttgttgttc ttttgtaatt ttaaacatag
     9181 taaaaaattt aaatgactat aattacataa tagaaaatgc agttaaaagt gaacagttaa
     9241 caaaaatcgg aaatacgata gaaacggtaa atgttacagg tttctcaagc tttagaaata
     9301 taatgggttc tacagaatcg tggtttatat ttggtataat tgcatgtatt tgctatgctt
     9361 tttttatttg gtatagagac tttaatggta ggagtaaatc tatatataca cttataatgc
     9421 tacctaaaaa tagaatcaat atttatatat ctaaattatt aaatatatta tttctagtgt
     9481 atagctatac agtagtactt acaatatctt tgtttattgc aagtaagttg ttgcctagac
     9541 atatgcttgg taatgtcaca aactatggtt ttgtacaaga aacaatatat gagttaaaaa
     9601 tgttacttcc atatagcttt gaaatactat ttgtagagta tatattttta ttgataggat
     9661 ttgtatctgt agtatttaca tcaattctta taaataaatc tatttataaa gtaagcactt
     9721 taataagttt tctattttta gttttggaaa ttttagtgtt tataatttct atagaattta
     9781 ttatacctta tgaatacagt gatgtattta ctgttctata ttcaagtata aacatatttg
     9841 tatgtagtct tatatctaat aaacttttga agcaaaagat tgacttttag gagggatatt
     9901 atgaaagcaa aaaatatagg aaaatataca atatgtttta taatagtatg ttttttaatg
     9961 attactatga ccttaatatt gagttatttt aataaggata aactatctgt aaaaacttta
    10021 aaaggcaatg taaaagatat agggagagta agtgtagtat atacacctaa tataagcaaa
    10081 ttttcttatg aggaggtaat tataagtaaa aatggagtaa gaaataataa aaaccccaaa
    10141 tcaagaatta gaaatgatta tttaaatatt gacaaaaaat acagtgattt tgttaatgat
    10201 aaaggaaaag aacttatata taaagatgat ttgaatatag gagaagcgta tacctctgtt
    10261 aagtctgatt tagtacaagg aatgaatgct ttaaatgatt gtataaaggt ttcaaataaa
    10321 aatttaaaaa gtggggtaga agaaaatttt gatattaatt taaataagtc tgattttaaa
    10381 gaaaacagtt ggaaaacctt gtttaataaa atatataaag gcaatttata tatggtaatt
    10441 agtgaaacta atatttcagg ttctaaggtc aataatattt gtatattaaa gatagatata
    10501 aaaaataagt cttataaatt attaaataga tttaatttag gattacagaa tggatatcaa
    10561 caaaatatca atgaaattaa attttctatt ggaagtaaat tatacttaga aatagtaaat
    10621 aataaaagta aaaatggaga tattgaagga catgatagtt actctaacct tattatatat
    10681 gatatagaag ataacacttt taatataagt aataaattta tggataatct aaacaaagaa
    10741 aagaatgcaa atccaaagga gattggctta ttaagtaaaa ctaagctaga ttatatgata
    10801 aaaaataata aattgaatct tatagttacg gatatgagta attccataat caatcttgtt
    10861 tatgatatta attctaatga gattaaatta gaagattatg agatacttga tttaaaagta
    10921 gattataatg aagcatcatc tggagttagg aaagttaagt tactggatga taagatttat
    10981 tctatttcag gtatgaagaa cagtatatca ggagaattaa aaggatttaa agggattgtt
    11041 gatgtagtcg gaactaagcc agtaaaattt caagtatttg atatgaaact taaaaagaat
    11101 atttatgaag ctgagctaat aaatggagat ataagtttac aagaaaagtt attttttgtt
    11161 gcaaattaag tattagaatt aattttagaa ctttaaactt ttatatgtat cacagataag
    11221 tataagtaag gg
//
LOCUS       DACTGW010000127        11195 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.127, whole genome shotgun sequence.
ACCESSION   DACTGW010000127 DACTGW010000000
VERSION     DACTGW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.127"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(355..3462)
                     /locus_tag="I9573_002460"
     CDS             complement(355..3462)
                     /locus_tag="I9573_002460"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAT4797070.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     gene            complement(3962..4489)
                     /locus_tag="I9573_002461"
     CDS             complement(3962..4489)
                     /locus_tag="I9573_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAT4797071.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(4524..5309)
                     /locus_tag="I9573_002462"
     CDS             complement(4524..5309)
                     /locus_tag="I9573_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAT4797072.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(5319..5576)
                     /locus_tag="I9573_002463"
     CDS             complement(5319..5576)
                     /locus_tag="I9573_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAT4797073.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFNILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(5595..6053)
                     /locus_tag="I9573_002464"
     CDS             complement(5595..6053)
                     /locus_tag="I9573_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAT4797074.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(6093..6797)
                     /locus_tag="I9573_002465"
     CDS             complement(6093..6797)
                     /locus_tag="I9573_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAT4797075.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(6807..7442)
                     /locus_tag="I9573_002466"
     CDS             complement(6807..7442)
                     /locus_tag="I9573_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797076.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IPVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            7823..8863
                     /locus_tag="I9573_002467"
     CDS             7823..8863
                     /locus_tag="I9573_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT4797077.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGIITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(9326..10681)
                     /locus_tag="I9573_002468"
     CDS             complement(9326..10681)
                     /locus_tag="I9573_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HAT4797078.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPISTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(10707..10976)
                     /locus_tag="I9573_002469"
     CDS             complement(10707..10976)
                     /locus_tag="I9573_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HAT4797079.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
BASE COUNT         3399 a         2033 c         1238 g         4525 t
ORIGIN      
        1 tcacgagtgt gcgtagctat tttttaatca atacttaact ttttgttttc accatacatc
       61 aaaacgcacc cctttgcata ataaataaac caccctgcag aacacacgat aaatttataa
      121 ataaatttat cagaagtagc ttactaaaaa tattattcat cctcacttaa acgtttattc
      181 attaatattc tatcacatga cataaataat ttatatgatt tatatagatt tatttattgt
      241 aaattttgga atattaaaac attttattga atagataatt atattacata aaaaatccct
      301 atagaaatct ataaactaat ttctataggg attttggtta actaactatt ctttttattt
      361 ttttataact tttatacctg tcatatggtc atttaagttt tgttcctcta aagatttatc
      421 atctactctg tttatttcta aagctaaagt ttcagacttg atatattcat taaaatggtc
      481 aacagcctta gcaatttcat catcaccaca gtattctatt actatattat ctaatacttc
      541 aaagttatta gattttctta attgctgtac tttagatata aattctctag cataaccttc
      601 attaatcaac tcttcagtta gcttagtatc tagtataaca aaaagattgt tttcaacaga
      661 tacattaaat ccttccttag cagttatacc tattaatact aaatccttat taaattcaaa
      721 agcttcgcca tctaaatcaa ctgttaaagt ctcacctttt tcaagtttat tagcagtttc
      781 atgagcattt aatttactta atgctccaac gaataaattc atcttagaac ctaataatgg
      841 ccctgcttcc ttaaagtttg gttttaaact aaagttcata tactcatcta aatccttagc
      901 aaatattacc tctttaacat taagctcttc ttttattaaa tcaactacat cacttattgt
      961 atcttcaaac ttaccatcta ctaacacttt ttgtaatggt tgacgaactt ttattctttc
     1021 tacttctctt gaagctctac ctaaagtaac taagttttta gctaaatcca ttttttcttc
     1081 taatttagta tctactaact cttcattaca tttaggatat tcagctaaat gaacagatac
     1141 ttctcctgtt aagtttctat acatttcttc tgacatatat ggtgcaaatg gagctatagc
     1201 tctacaaaga ccatgtaaca attcataagt tgtattgtac actgcttttt tatcttctgt
     1261 taattcagtt gcccagaatc ttcttcttga acgtcttata taccagttag ataaatcatc
     1321 atttataaaa tcttgtatca tccttaaagt tttatttaac tcaaatatag ctaaattttc
     1381 ttctacatct ttcattaaat tattgaactt agataatatc catctatcaa gttctggtct
     1441 atctttatat tctacaaaaa attcagttgg atttatatca tctgtgtttg catataaagt
     1501 aaagaagtta tatacattct tcatagtacc tataaattta ctttggattt cttttaatcc
     1561 atccatatca aatttagttg gagtccatgg tggagaaaca tatagtaaat accatcttaa
     1621 tgcatctgct ccatattggt caaacaattc cattggattt actgtatttc ctcttgattt
     1681 actcattttc ttaccatctt tatcaagtac aaggtctgga actaaaacac tcttatatgg
     1741 tgcttttcct aatacaaatg ttgatactgc aagtaatgag tagaaccaac ctctagtctg
     1801 gtctatacct tctgatatat agtcagctgg gaacaattgg tcaaaatttt ccttattttc
     1861 aaatggatag tggtgttgag caaatggcat agccccacta tcaaaccaac agtctataac
     1921 ctctgttact cttgtcattg gttttccaca tttttcacat tttaagtgta tatcatctac
     1981 atatggtcta tgaagttcaa ctgtttctgg atttacatct tctatagctt tttcagctaa
     2041 ttcagctctt gaaccaactg aatctgtatg accacattca catctccata taggaagtgg
     2101 agttccccaa tatctagttc ttgatattgc ccagtcattt agattttcta accagtttcc
     2161 aaatctcttc tctcctacaa aacttgggaa ccattttaca ccattattat tttctattaa
     2221 tttatcttta agtttagtca tctctatata ccagcttggt tttgcatagt atactaatgg
     2281 agtttgacat ctccagcagt gtgggtaatt atgaactact ttttctttgc tatataattt
     2341 attttcagaa gctagccatt ttattatttc aacatctagc ccatcttcca ttacaaatct
     2401 accttcccaa ggagtagatg taaatttacc agtttcatct actggttgaa taactggtaa
     2461 atcatatctt ttacctgtgt tgtagtcatc ttcaccaaat gctggtgctg tatgaactat
     2521 accagttcca tcttcagtag ttacataatc tcctactgta ataaaaaatg cttttttgtc
     2581 aacttctaca aaaggcatta attgttcata ttctaaacct tctaggtctt tacctttcat
     2641 ttcttctaaa acttcataat cttcacctag tactttatca gctaaaggtt tagatacata
     2701 atatacctca tcaccttttt taacttttaa ataatctaca tcagcattta ctgttaatga
     2761 aacatttgat ggtaaagtcc atggagttgt tgtccatgct aagaagtatt catcagcatc
     2821 ttttttctta aatttagcta taactgtatt agtttttact tctttatatc cttgtgcaac
     2881 ctcatgacta gcaagaccag ttccacatct tgggcaataa ggaagtattt tatgcccttc
     2941 atatatatat ccttctttgt tgaatttgtt tagtatccac catacagact ctatatagtt
     3001 attatctaaa gttatatatg gattatctaa atcaacttca taagccattc tctcagtcat
     3061 tattctccat tgtttttcaa atgagaaaac tgactctcta catttttggt taaattcagc
     3121 tattccatat tcttctattt gttgcttgct agtaagacct aactctttct ctacttgtat
     3181 ttcaactggt aatccatgtg tatcccaacc agcttttctc tttacttgat aaccagacat
     3241 tgttttatat ctacaaactg aatctttaag cgttctagat aaaacatggt gtactccagg
     3301 atttccatta gctgttggag gtccttcata gaatacgaaa cttgggtcat ctttcccttt
     3361 ttctaaagtt ttttcaagta tatttatgtc tttccaataa tcaaaaactt gtgcttctgc
     3421 ttgttttaca gaactgtcta ccaatggctt aaatttcgcc atgtaaatca ccctttccta
     3481 ctaaatatta taattccaaa attttttatt ctttcacata aatttaaata ctaaaaaact
     3541 cgcccccttt aaaccaaagg gacgagttat aaatcgcgtt accaccctaa ttctatatgt
     3601 aaactaaata ctagctacat ataacactct aatagtataa cggtactaac cggcacagct
     3661 tagtagaatt taatcgttca gcctgcagct caagagtgat ttttatcttt ataaattcat
     3721 tggttctcag catacccaat tctctgtaaa attatctaaa gataaatgtc tctatcaacg
     3781 cttttaaaaa tttatctgtg tttatatgaa attttataac aaataatttg aatagtcaat
     3841 atatttttga aaaatataat aatttctatt tttcttacat ttttatccta tttttaatct
     3901 ttgttataga tttctcaaac tcacacagtt attctacata aaaaataaac aaattacaag
     3961 cttattctaa agttgtagca gcttcatcat tactataatt atacactgtt gttccttcaa
     4021 atgcatttac acatttttca tctacatcat aaaaaatctc atcaacactt cttatttcat
     4081 cttctaacag tgatttaaat ttatttctaa atatcttaaa ttctttgact aaagcatcat
     4141 actcttttct tatctctatg acacggttat tagcttgttc tataatttgt ctagctttta
     4201 aatcagcttc ttcaacaatt atcttagcct ttttattagc cgcgctacaa gtgtcttctg
     4261 ctgcactttg agcagttatt aaagtagctt ttagtgtttc ttcaatattt tcatacttgt
     4321 ttacttggtc ttgatataac cctaattttt ctttaagagc tgtattttcc ctacatagag
     4381 attcataatc ttccttgatg atatctaaaa attcatcaac ctcttcatct ctataccctc
     4441 ttaaaccttt tttaaactct ttattttcta tctcaattgg agttagcatt acatttcctc
     4501 ccagctataa tttaaattag tccttataca attaatcttg cttgaacttt tatttttcct
     4561 tttttggtaa tatctcctat ttgtgttacc ttagctcttc ctttgcctct aatagaaatc
     4621 aaatcatcat tttttactat tttagaagtt gaagttatct tttcataatt gacatggact
     4681 ttttctccat ttatgtattt tgcactatct tgtctagata tattgtaaat accacttatc
     4741 acacaatcta acctatctga agatactgta aatgatactt ctttatattt tgaagaacta
     4801 ttaacaatat tttctctgga aatttccttt acaattacat tatttcttga aaccttttct
     4861 aagtttacaa ttataaaatc gcaaatatca ctacttacaa ctacttgaca aaagctatca
     4921 tgtataatta tatctccaat tttttctctt tttataccta gacccagtat tgaccctaag
     4981 taatctttat gagatatatt tttaaacttg aaattccctt ctatttgtaa aaatctaaga
     5041 gtatcttcta tatcttcata ttccatgtaa aatggataaa taaatacagt gcttctttca
     5101 gcttgttcat aacctccatc cacactgtat tttatttcat ttgtagaatt taatattgct
     5161 acagcatttt tcacttcata tggattaaga aactccgtag acttaacatc atagtttttt
     5221 atacaactat tagccttgtc tatcacttta aacattttat ttttcaagtc tatatccttt
     5281 atgtgatttg ttagttttaa tttatccatc ctatcacctt agaatataac caatactaat
     5341 tgtcttagga acatcaataa tactattgca attaaaggag aaaaatctat aggtccattt
     5401 gtatatttat acattataga ccttattggc ccttctattg gctctgtaag attttccaaa
     5461 atattaaaaa ttttactttg tcttccattt ggaaaccaag tcatcaagct ttttataact
     5521 atcatccatg ctattatatc taaaagataa taaagtgcaa ttcttattgt tcccatatat
     5581 taaccgcctt tttactattt attttgccaa gggaagaatg ctttactttc taattctttt
     5641 ttcatatttg catctatatc cacattatta ggagctagta taaatattga tttagaaacc
     5701 ttttgtatac ttccatctaa tacataaact gccccattca taaagttaaa tatagattta
     5761 cgaacttcag aattagtcaa tccttccaaa ttcactataa ctgcccttct ttgttttaga
     5821 tggtctgcta tagttgcagc ttcatcataa accttaggtt caactattac aactttcatt
     5881 tggctagttg tatgtaaatt tacaatttta tttgcttttg cagtactaaa gccactgttc
     5941 atttcttctt cttgaactat atcgtccatg ccactttcat attcatcttc aatataatct
     6001 tcttcttcat caactatcca attcttaaat ttagatatta ttccatttga cattattaac
     6061 ttcctcctta aaatttattc aaatttgagt aattattttt tattgtagtt tctttctcca
     6121 aagattgatg ttccaactct aacaatggta gccccttcct ctatagcaat ttcataatcg
     6181 tgactcattc ccattgacaa aacatccatc cctacattag ggatgtttag ttcttttatt
     6241 tgttttgata aatctttaag acctttaaat acacctctta tctcatcttc atcttctata
     6301 aaaggtgcca ttgtcattag accctttatt tttatatttt tatatttttc tgacacttct
     6361 ttgacaaaat ctacaacttc ttctctatca agtccatgct tagactcttc tttagatata
     6421 ttcacttgta ctaagcaatc tatttctctg tttatttgtt ctgcacgttt ttgaatttct
     6481 tcgcataatg caattctatc taaagagtgt atcatacaaa ctttatcgat tatgtactta
     6541 actttattgg tttgcaaagt tcctatcagg tgccagttta atttgtcacc tataatatca
     6601 tactttctag ctaattcttg gggtttattt tcaccaacag aagtgattcc ttccctcatt
     6661 gcctcattaa ctaagtctac atcaacagtc tttgtgactg ccaaaagggt aatctctttg
     6721 ctgtctctat ctactttttt tgcagcttta tcaattttgt ttcttatctc agacaaatta
     6781 tctttaatag aacccattat tatcacctac tttatcctta tatttgtgtt tatactgtta
     6841 ggtcttaaaa ttattttgtc atatagatta acacttgtaa ctccatttat gtcattttta
     6901 taataatcca caaatacaga agattcattc tcataagtta ttccttctaa tatgacaaat
     6961 tttacacttt gtgattgttc atcaattaca tatacaccac gtttattgtc ttttgttttt
     7021 atagaagttt taggtatttt taaacattcc atctgattat atattatatc aaattcttcg
     7081 acacttgtat cctgatttgc aacaatttgg ttacttattt ttaattttac cattatttta
     7141 ttattatttt catatatttt gtaaactttt gcatctaatt tttgctcatg atattcaatt
     7201 attacatttt gatttttttc aaagttatta gaatcttctt tactgatgta tgtagataga
     7261 taattacttt ggtcattggc atctttttct atagttcctt ttacaggaat ctttgcatca
     7321 actttttcat tttttaacac caacgtatct acatttttgc taattatcat tactaatata
     7381 ttaaatacta aatatataag aataccaagt atcactaatc tggtatattt tacctttttc
     7441 aaagactctc cccctataaa attactttca ttataagtac ttcttctaaa aaatgtttat
     7501 tcctttttta taagctaata ttttacgttc taataattat tttttccata ttaaatttat
     7561 tttttatcaa tcttcctata caagactgca ttatttatat ataattgtat tataaagtat
     7621 aagtttaatt tacattttat acattaagct tactttatat gtttttatag ttatttttat
     7681 ttttagatgc aatataaaac caattaagat ttaaaataac ttttgttatg ttgacattta
     7741 tttaggtaat catttacgaa tcaggataac gttatcataa caaaagttta tgtcctaaaa
     7801 aaaatacaag gaggattttc ttatgtcaga aattttatta ggaacaggtt tacttttatt
     7861 ggttctatgt tgtttcacaa tttttagtta caaggcccca tatggtatga aggctatggg
     7921 agctctagca aacgctgcat gtgctacttt cttagtagag gcattcaacc tttctttact
     7981 tggtgatgtt ttaggtatgg aattttttag aagtgttgga gctgctaatg gtagtcttgg
     8041 tggagttgct gctgctatac ttgtgccttt agccttaggt gtatctccag tttatgcagt
     8101 tttaatagga ttatcttgtt tagatttttc tatattatct ggattcgttg ctggatactt
     8161 tgtttctttc ttaattaagt ttatagagaa aaaagtgcca cctggagtgg atttagtggt
     8221 tgtaattctt ctatgtgcac cgctatctcg tcttatagca tcaaatgtag acccattagt
     8281 taatagtaca ctactacaaa ttggacaagt tttgatagaa gctaccaaca ttagccctat
     8341 aataatggga attatactag gaggcataat aacagtagtt ggaacgtctc cacttagttc
     8401 aatggctctt acatctataa ttggtcttac tggtcttcct atggcaatag gagcattagg
     8461 tgcttttggt tcagctttta tgaactcaac actatttgca aaattgaaat taggttctaa
     8521 aaaagatatt atatctgttg ctattgaacc attaacccaa tcagacataa tttcagctaa
     8581 tccaatacct atatattcta ctaatttctt tggaggagcc ttttctggta tcattgtagc
     8641 cttgatgggt cttgtaaaca tgacaccagg tacagctaca cctgtggctg gttttgctat
     8701 aatgtttgct tataatccag ttcaacaagt tcttataaca gctggtttat gtgctttatt
     8761 caatgtaatt gcaggatttt taggttcaaa aatattcagt acctttaaag ttcttactgc
     8821 tggtgaaata cgtggtactg atgtagaaga agaaattgct taaaaaacaa taagatttat
     8881 aaatttatat ctcaagtagt agagttttat attgtaggca attttattaa ttgcaaacgt
     8941 tttatttttt atttatgttt atgattatta tgtgtgttga atatttagat aaatttttat
     9001 ttattatttt tatttagatt tttgtttagt tttttatttg gttgatatat taggaataaa
     9061 aattagtata gtttattatt agttagatag cataactttt tagatacata aaaaatttta
     9121 tattaattag atattattaa aataaaagcg atacatgaat ttttaaatgt ccatgtatcg
     9181 ctttattttt gccataatct taacaactga tttttaaagt ttattatctt aacgaagttc
     9241 attatcttat ataatattta aatgcataga cttctttaat ttaaccatct aaatatttaa
     9301 ttaaatatta ttatatgtaa tataactaat ttttaaatga atgtatagga gctggaatcc
     9361 ttcctcctct ttgagtaaat aattcagatg aatttgtact tataggcatt actggtgctc
     9421 gtcctaaaag tcctccaaat tctaccatat cacctacatc acatcctggg gctggtataa
     9481 ttctaacggc tgtagtctta ttattaatca tacctattgc tgcctcatca gctatcatag
     9541 ctccaagcgt acctgctgta gtatttcctg gcacagctat catatcaaga cctacagaac
     9601 aaatagcagt cattgcttca agcttatcta tacttaaagc acctttaata acagcatcaa
     9661 tcatacctgc atcttcagat actggtataa acgctccact aagtcctcca acatgtgaac
     9721 atgccattac tccacctttt ttaacagcat catttaaaag tgctaatgct gctgtagtcc
     9781 catgagtacc aacaacttca agacccattt cctctaatat atttgctact gaatcaccaa
     9841 tagcaggtgt aggagctagt gataagtcta ttatcccaaa tggaacatcc aatctactag
     9901 aagcttcctt tgccacaagt tgtccaactc ttgtaatttt aaatgcagtc tttttaactg
     9961 tttctgaaac aacatcaaaa ggctctccct ttactttctc taaagctctc ttaacaacac
    10021 cagggccact tacaccaaca tttataattc tatcagcctc accaacacca tggaaagctc
    10081 ctgccataaa tggattatct tctactgcat tacaaaatac tactaatttt gcacaaccaa
    10141 aaccttttgc atcttttgtt aattctgcag tttcttttat gatttctccc atttctttca
    10201 ctgcatccat atttatacca catcttgtag aacctacatt tactgaagca catactttat
    10261 tagtacttgc tagagctttt ggaattgact taattagtat cttatcacct tttgtatatc
    10321 ccttttgaac taatgctgag aatccaccaa taaaatctat acctaaatcc tctgcagcct
    10381 tatccaatgt ctgtgcaaac tttacatagt cattttcatc tgttgcacct gcaataatgg
    10441 aaattggagt tactgatacc cttttattta caattgggat accaaattca cgctctatat
    10501 cttttccaac cttcaccaaa tctttagcag aactcataat cttattgtat attttttctc
    10561 tagctttatc tccatcaggg tctatacaat ctaataaaga aatccccata gttatagttc
    10621 ttatatccaa ctgttcttca tctatcatct ttattgtttc aagtatattt cctacattca
    10681 ctattatcac ctccccttac tgtctactac agattatgca ttgaattaaa tatttcttct
    10741 cgctgaattt ttacatccac tgataatttc ttacctcttt caaccaagtc ctcaaccgtt
    10801 ttctcaaaag aatttaatga tttctctaaa tcaactatca taatcatagt aaaataatcc
    10861 tgtaatattt tttgtgatat atctactatg ttgatatttt gcttgtataa ttcatcacta
    10921 attcctgcca ctatacctgg tttatccttt ccaattactg ttaaaattgc tttcataata
    10981 atatcctccc ttattttttt ataaatctta tatttttaca aattgaacta tgtattaaca
    11041 tatatttcat ttatttgtta gtaaaccact ttttatgtat atatttaata tattttaata
    11101 cctatacatt catttgtcaa ttttttttca atattccatt atttttatac tatttagaaa
    11161 attcatattt tatatatgtt ttctcaacaa taaat
//
LOCUS       DACTGW010000128        11074 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.128, whole genome shotgun sequence.
ACCESSION   DACTGW010000128 DACTGW010000000
VERSION     DACTGW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.128"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            340..4056
                     /locus_tag="I9573_002470"
     CDS             340..4056
                     /locus_tag="I9573_002470"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAT4797080.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRKLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGQSFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     VEATEDEDIENLEEFTEDDLFEEEIDFESDDFDM"
     gene            4098..7583
                     /gene="rpoC"
                     /locus_tag="I9573_002471"
     CDS             4098..7583
                     /gene="rpoC"
                     /locus_tag="I9573_002471"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAT4797081.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVLVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            7872..8294
                     /gene="rpsL"
                     /locus_tag="I9573_002472"
     CDS             7872..8294
                     /gene="rpsL"
                     /locus_tag="I9573_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAT4797082.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            8417..8887
                     /gene="rpsG"
                     /locus_tag="I9573_002473"
     CDS             8417..8887
                     /gene="rpsG"
                     /locus_tag="I9573_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAT4797083.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            8936..11002
                     /gene="fusA"
                     /locus_tag="I9573_002474"
     CDS             8936..11002
                     /gene="fusA"
                     /locus_tag="I9573_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAT4797084.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFINKMDILGADFYNVISMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         4281 a         1408 c         2304 g         3081 t
ORIGIN      
        1 ctttattttg tatattttaa taaattgtat atatttgtat aaatgtaatt atttacaatc
       61 attttaatat ggaattataa ttaaataggg gggatggtac ttgtataaaa ttttttatag
      121 aaaattagac ctataaatga aaaatatgga ttttatattg gaaaattgta cataatacat
      181 aatatatgct taaaataatt tacaaaaaat caaaaataat gcatagttta catataagat
      241 aaaaaatata ttgaaaaata cttgacatta tgctaaaatt attagatgcg ttagaatgaa
      301 ctattgtttt atttttaatt attgagaggt gaaaagtgaa tgccacatcc tgtcacgata
      361 ggtaaaagaa ctagaatgag cttttctaaa attaaggaaa tagctgatgt gccaaatctt
      421 atagaaattc aagtagattc ctatgagtgg tttttaaaag aaggtttaaa agaagtgttt
      481 gatgatattt caccaataga agattatact ggtaatctta tattggaatt tgtcgactat
      541 tctctagatg ataaaccaaa gtacgatata gaagagtgta aagagagaga tgctacatat
      601 tgtgcacctc taaaggttaa ggtaagactt ataaataaag aaacaggtga gataaaagaa
      661 caagaagtgt ttatgggtga cttcccttta atgacagaaa gaggaacttt cgttataaat
      721 ggagcagaaa gagttatagt aagtcaatta gttagatctc ctggtgttta ctatgcggaa
      781 gaaagagata aaacaggtaa gagattgatt tcatctactg taattcctaa tagaggagca
      841 tggttagaat atgaaacaga ttctaatgat gtaatatctg taagagttga tagaacaaga
      901 aaacaaccag ttacagtttt acttagagct ttaggaatag gaacagatgc agagataatt
      961 gaccttttag gagaagatga gagattatct gctacattag aaaaagataa tactaagaca
     1021 gtagaagaag gtcttgtaga gatatacaaa aaattaagac caggtgaacc tcctactgta
     1081 gaaagtgcat catctttatt aaatgcttta ttctttgacc caaaaagata tgacctagca
     1141 aaagttggta gatataaatt caataagaaa cttgctttat gttatagaat aatgaataaa
     1201 atttcagcag aagacataat aaatccagaa actggagaag tatttgtaaa agctggagaa
     1261 aaaataagct atgatttggc taaagccata caaaatgcag gtataaatgt agtaaatcta
     1321 ctaatggatg atgataaaaa agttagagtt ataggaaata attttgtgga tattaagtct
     1381 cacattgatt ttgacataga tgatttaaac ataaaagaaa aagtacatta tccaacttta
     1441 aaagaaattt tagatggata tagtgatgaa gaagaaataa aagaggctat aaaatcaaga
     1501 attaaagaac ttataccaaa acatatatta ttagatgata taatagcttc aataagttat
     1561 gagttcaata tattctataa tataggaaat attgatgata tagatcacct aggaaacaga
     1621 agaataagat ctgtaggtga attgttacaa aatcaagtta gaattggtct ttcaagaatg
     1681 gaaagagtta taaaagaaag aatgacggtt caagatatgg aagctataac gcctcaagcg
     1741 ttagttaaca taagaccagt ttcagctgca ataaaagaat tttttggtag ttctcagtta
     1801 tctcagttca tggatcaaac aaatcctcta tctgagttaa cgcataagag aaaattatca
     1861 gcccttgggc ctggaggtct ttcaagagaa agagctggat tcgaagtgcg tgacgttcac
     1921 cattcacact atggtagaat gtgtccaata gagactccag agggaccaaa tatcggtctt
     1981 ataaactctc taggaactta tgcaaaaata aatgaatttg gatttataga atcaccatat
     2041 agaaaatttg ataaagaaac atcaacagtt actgatgaaa tacattattt aactgctgac
     2101 gaagaagatt tattcgtaag agctcaggca aatgagccat taacagaaga tggtaaattc
     2161 gtaaaccata gagttgtttg tagaactgta aatggtgctg ttgaaatggt tcctgaaagt
     2221 agagtagatt acatggatat atctcctaag caggttgtat ctgttgccac tgctatgata
     2281 cctttcttag aaaatgatga tgccaaccgt gcccttatgg gagcgaacat gcagcgtcag
     2341 gcagtgcctc tagttagaag agaagcacca attataggaa ctggtataga atacagagca
     2401 gcaaaagact ctggagcagt tgttgttgct agaaattctg gtatagcaga aagagtaaca
     2461 gcagatgaaa taattataaa aagagaagat ggaaacagag atagatataa tctacttaag
     2521 tttaaacgtt caaactcagg tacttgtata aatcaaacac ctataataaa taagggtgac
     2581 cagataataa aaggtgatgt tatagcagat ggtccagcaa ctgatttagg agaagttgca
     2641 cttggaagaa actgtcttat agcattcatg acttgggaag gttataacta cgaggatgct
     2701 atattaataa atgaaagatt agttaaagaa gatagattat caacaattca tatagaagaa
     2761 tatgaatgtg aagctagaga tacaaaacta gggccagagg aaataactag agatatacct
     2821 aatgttggag atagtgcaat caaaaactta gatgatagag gtataataag aataggtgca
     2881 gaagtagact caggtgatat attagttggt aaggtaactc caaaaggaga aactgaactt
     2941 actgctgagg aaagattact tcgtgcgata ttcggagaaa aagccaggga agttagagat
     3001 acttcactta aagtacctca tggtgaatct ggtataatag ttgatgtaaa agtatttact
     3061 agagaaaatg gagatgattt atctccagga gtaaatgaat tagtaagatg ttatatagct
     3121 aaaaagagaa agataaaagt tggagataag atggctggtc gtcatggtaa taaaggggtt
     3181 atctcgagag tattacctga agaagatatg ccattcatgg aaaatggaac gccattagat
     3241 ataatactta acccacaagg tataccatca cgtatgaaca tcggtcaggt tctagaagtt
     3301 catttaggac ttgctgctaa gacattagga tggtatgtag caacatcagt attcgatggt
     3361 gctaatgagt atgacataat ggatgctctt gaagaagctg gatatcctag agatggtaaa
     3421 ttaactttat atgatggtag aacaggtcaa tcttttgaca atagaataac tgttggatat
     3481 atgtattact tgaaactaca tcacttagta gatgaaaaac tacatgcaag aagtactgga
     3541 ccatactcat tagttactca acagccacta ggtggtaaag cacaatttgg tggacaaaga
     3601 tttggagaga tggaggtttg ggctctagaa gcatatggag cggctcacat acttcaagag
     3661 atacttactg ttaagtctga tgatgttgta ggtcgtgtta gaacttatga agctatagtt
     3721 aaaggtgaaa acatacctga gccaggaatc ccagaatcat ttaaggttct tataaaggaa
     3781 cttcaaagtt tatgcttaga tgtaaaagta ttgacagatg aagaccaaga aatagaagta
     3841 agagaatctg tagatgaaga tgatacgata ggtgaatttg agctagacgt tgtaaatcac
     3901 atgggagaag ttgaagaaag taatataata gaagaaattg aagatgattt tgtggaagcc
     3961 acagaagatg aggatataga gaatttagaa gaattcactg aagatgattt atttgaagaa
     4021 gaaatagatt ttgaaagtga tgactttgat atgtaaattt aattaatgta aatctcaaat
     4081 agaagggaga gaactccttg tttgaattaa acaatttcga gtcgataaaa atagcattgg
     4141 cttctccaga aaaaataaga caatggtcta gaggagaagt taaaaagcca gaaactataa
     4201 attaccgtac tttaaagcca gaaaaagatg gtcttttctg tgaaaggata tttggaccac
     4261 aaaaagactg ggagtgtcat tgtggtaaat atagaagagt tagatataaa ggtgtagttt
     4321 gtgatagatg tggagtagaa gtaactaaat caaaagtaag aagagagaga atgggacata
     4381 tagagctagc tgctcctatg tctcacatct ggtacttcaa aggtatacca agtagaatgg
     4441 gacttttact tgatatgtca ccaagatcgt tagaaaaaat actatatttc gcctcatatg
     4501 tagtagttga tccaggtgaa actggattga atgaaaaaca gttacttaca gaaaaagaat
     4561 acagaactgc tcttgaaaag tatggatata cttttactgt gggtatgggt gctgaagctg
     4621 taaaaacatt actacaaaat atagatttag aacaacaaag taaagactta agagcggaat
     4681 taaaagatag tacaggacaa aagaaagtaa gaacaattag aagattagaa gttgtagaag
     4741 catttaaaaa atctggaaat aaaccagaat ggatgatttt agatgcaata ccagtaatac
     4801 cgccagattt aagaccaatg gtacaacttg atggtggaag atttgcaact tcagacctaa
     4861 atgatttata cagaagagtt ataaatagaa ataatagact taaaagacta ttagagcttg
     4921 gagctccaga tataattgta agaaatgaaa aaagaatgct tcaagaagct gttgatgcat
     4981 tgatagataa tggtagaaga ggtagacctg taacaggacc tggaaataga ccacttaaat
     5041 ctttatcaga tatgttaaaa ggtaagcaag gtcgtttccg tcaaaactta cttggtaagc
     5101 gtgttgacta ctcaggacgt tctgttatag ttgttggacc agaacttaaa ttttatcaat
     5161 gtggacttcc aaagaagatg gcattagaat tattcaagcc atttgttatg gataaattag
     5221 ttaaagaagg ttatgcacat aatataaaaa gtgcgaaatc tatagtagaa aaagttaagc
     5281 cagaagtttg ggatgtttta gaagatgtta taaaaagtca tccagttctt cttaaccgtg
     5341 caccgactct acatagatta ggtatacaag catttgaacc aatcttagtt gaaggtaagg
     5401 ctataaaact acatcctctt gtatgtacag cttataatgc agactttgat ggtgaccaaa
     5461 tggcagtcca tgtaccttta tcagtagaag cacaagcaga agcaagattc ttaatgcttt
     5521 ctgtaaataa tatacttgct cctaaagatg gttcacctat aactactcca tctcaggata
     5581 tggttttagg ttgctattat ctaacaatag aagcccaaga tggagctaaa ggaacaggta
     5641 tggtatttaa agactttaat gaactattac ttgcttatta caataaatca gttcatctac
     5701 atgcattagt gaaactgaag gtaacacttg aagatggaag aagttcattg gttgaaagta
     5761 ctgttggtag atttatattt aatgaaaata tacctcaaga cttaggtttt gtagatagaa
     5821 aagaaaatcc atttgcactt gaagttgatt tcttagcaga taaaaaatct cttggtaaaa
     5881 taatagataa atgctttaga aaacacggaa atacagaaac agctgaatta ctagattata
     5941 taaaagctct aggatttaaa tattctacat taggtggtat aacagttgct gttgatgata
     6001 tgagtgtccc agaagaaaag aaagtattta tagctgaggc agaagctaaa gttgataagt
     6061 atgaaaaagc atacagaaga ggtctaatct ctgatgaaga aagatatgaa aaagttatag
     6121 agacatggac agaaacaact gataaagtta ctgatgctct tatgggtgga ctagatagat
     6181 taaataatat atatataatg gcacattcag gagccagagg ttctaaaaac caaattagac
     6241 agctggcagg tatgcgtggt cttatggcaa atgcatctgg taaaacagtc gagataccag
     6301 ttaaatctaa tttccgtgaa ggtttatcag tactagaata ctttacatct tcacatggtg
     6361 ccagaaaagg tcttgccgat acagctatac gtacagctga atctggatat ttaacaagaa
     6421 gacttgttga tgtaagtcaa gatgttattg taagggaaat agattgtgga acagaagata
     6481 ctacagagat ttatgctata aaagaaggaa atgaagttat agaagagata tatgatagaa
     6541 ttgtaggaag atatactata gaccctatat taaatcctga aactggagaa gttctagttg
     6601 aggctgattc aatgatacaa gaagatgaag cagaaactat agtagcttta gggattgaaa
     6661 aaattaggat aagaacagtt cttaactgta aaactaatca tggagtttgt tctaagtgtt
     6721 atggtagaaa cttggcaaca ggaaaagaag ttaatatagg tgaagcagtt ggtataatag
     6781 cagctcaatc tatcggtgag ccgggtactc agcttacaat gcgtacattc catactggag
     6841 gagttgcagg agctgatata actcaaggtc taccaagggt tgaagaatta tttgaagcta
     6901 gaaaaccaaa aggattagct gtaataactg aagtatctgg tagagttgaa attgatgaaa
     6961 caggaaagag aaaagaagta aatgtaatac cagaagaagg cgagactcaa acatatgtaa
     7021 ttccatatgg ttctagatta aaagttaagc aaggtcaaat gctagaggct ggagaccctc
     7081 taacacaagg atttataaat cctcatgata tagtaagagt aaatggtgtt aagggagttc
     7141 aagaatatat agttaaagaa gttcaaagag tgtatagact tcaaggggtt gatgttaatg
     7201 ataagcatat agaagttata gtaagacaaa tgctatctaa agtaaaagtt gaagacccag
     7261 gagatacaga tttattgcca ggtggatatg aagatgtatt aacattcaat gaatgtaata
     7321 aagatgctat agataaaggt ctaagaccag cagttgctaa aagagtttta cttggtataa
     7381 ctaaagcatc tcttgcaact gattcattct tatcagcagc ttctttccaa gaaacaacaa
     7441 gagtattaac agaggcagca ataaaaggta aagaagacca cttaatagga cttaaagaaa
     7501 atgttatatt aggtaagtta ataccagcag gaacaggaat gaagaaatat agaaatatag
     7561 ctgttgaaaa aattgaagat taatagcata tagagtaaag aaatcttgac aaccattatt
     7621 tgtggtgtta aaatagtaaa gtgtgttgtt taaaagtaca ctaagggctt gaacaagttc
     7681 ttagtgactt ttagatggca ataaatgaga actaaaaagc atcaagctag aaaagtgcta
     7741 atatcacata tttatgtgtt atagacatta aattgtaagt aacgaatgtt acaatcagtc
     7801 agtatgaata ttcatattga tgtagttggc ggtcactgat aagatcgaaa atttggaagg
     7861 aggtgcagat gatgccaaca attaaccaat tagttcgtaa aagtagaaaa gcgctagaga
     7921 agaaatctac tgctccagca ttacaaaaag gttacaactc tttaaataag aaagtaactg
     7981 acgctagtgc cccacaaaaa agaggggttt gtacttcagt aaaaacagtt actcctaaga
     8041 agcctaactc agctttaaga aaagttgcca gagttagatt aactaatggt atagaagttt
     8101 ctgcttatat accaggagaa ggacacaact tacaagagca tagtgttgtt cttataagag
     8161 gaggaagagt aaaagacctt ccaggggtta gataccatat actaagaggt actctagata
     8221 cagcaggtgt tgataagaga agacaatcaa gatctaaata cggtgcgaag agacctaaag
     8281 aagctaaaaa ataaaacaat taatgattga ttatagttcg gtgctttaaa tatatatata
     8341 tttacggcat cacggcatta aatgatttat gtcgagtacc tatgatttaa gttaaataat
     8401 taaggaggga agcgaaatgc caagaaaagg taatattcca aaaagagaag ttttaccaga
     8461 tcctatgtat gggagtaagg tagttactaa attaataaat aatctaatgg tagacggaaa
     8521 aaaaggaaag tctcaaagaa tagtttatga tgctttcgca attgttgctg aaaagacagg
     8581 agaagaagct ttagaagtat ttaataaagc aatggataat ataatgcctg ttttagaagt
     8641 aaaagcaaga agagttggtg gggcaaatta ccaagtgcca atcgaagtta gacctgaaag
     8701 aagacaaaca ttaggtttaa gatggctagt aaaatatact agagctcgtg gagaaaaagg
     8761 aatggttgaa aagctagcta aagagataat ggacgcagct aacaacactg gagcttcagt
     8821 taagaaaaaa gaagatactc ataaaatggc agaagctaat aaagcatttg ctcattacag
     8881 atggtaggat atatagatat cttataaatt caaggatatc ataaggagga taaccatggc
     8941 tagaaagttt cctttggaaa aaactaggaa tataggaatc atggctcata tagatgcagg
     9001 aaagactact actacagaaa gaatcctgtt ttatactggg caaactcata aaataggaga
     9061 aactcatgaa ggagcttctc aaatggactg gatggagcaa gaaaaggaaa gaggtataac
     9121 aataacttct gccgctacta ctgcttcatg gaaagaccat agaataaaca taatagatac
     9181 tcctggacac gtggacttca cagttgaggt tgaaagatct ctaagagttc ttgatggttc
     9241 agttgctgta ttctgtgcaa aaggtggggt tgagcctcaa tctgaaaatg tatggagaca
     9301 agctgaaact tatggagtac ctagaatagc ttttataaac aaaatggata tattaggtgc
     9361 agatttctac aatgttataa gcatgatgaa atctagatta aattctaatg cagttcctat
     9421 gcaattacca ataggtaaag aagattcatt tataggtata atagacttat taaaaatgga
     9481 tgctgttata tacaaagacg acctaggggt tgaaatggaa gaaacagata taccagaaga
     9541 tatgaaagaa ttagctgctg agtggagaga aaaattagtt gaatcagtag ctgaaactga
     9601 tgaagaatta atgatgaaat accttgaagg tgaagaactt actatagatg aattaaaagt
     9661 tgctataaga aaagctacta tagcttgtga aatgaaccca gttttctgtg gtacagctta
     9721 tagaaacaaa ggtgttcagt tagtaataga tgcagtttta gattacttac cagcaccaac
     9781 tgatatacca gctataaaag gtatattagc agatggagaa gaagctgaaa gacattcttc
     9841 tgatgaagag ccattctctg cattagcatt caaaataatg actgacccat tcgttggaaa
     9901 actagcgttc ttcagagttt attctggaac attagaatca ggttcttatg ttttaaatgc
     9961 aactaagaat aagagagaaa gaattggtcg tatattacaa atgcatgcta atacaagaga
    10021 agaaataact aaagtgtatg ctggagatat agcagcagca gtaggattaa aagatactac
    10081 tactggagac acattatgtg accctgctaa tccaataata cttgaatcta tggaattccc
    10141 tgagccagtt atatctgttg ctatagaacc aagttctaaa gcagctcaag aaaaaatggg
    10201 tatagctctt caaaaattag ctgaagaaga cccaactttc acagttaaga ctgaccaaga
    10261 aacaggacaa actataatat ctggtatggg tgaattacac ttagagataa tagtagatag
    10321 attattaaga gaatttaaag tagaagctaa agttggtgca ccacaagttg catatagaga
    10381 gactataact caaccagttg atgttgaata caagtactct aagcaatcag gtggtagagg
    10441 acaatacggt cacgttaaga ttagagttgc tcctcaagag ccaggtgaag gatacaagtt
    10501 cacaaacaaa actgttggtg gatcagtacc aaaagaatat gttggtccag ttgacatggg
    10561 tatacaagga gctatgcaat ctggtatagt tgctggatat ccagttgtag acgttgctgt
    10621 tgagttatat gatggttctt accatgaggt tgactcttct gagatggcgt ttaagatggc
    10681 tggttcaatg gcattcaaag atgctatgaa gaaaggtaat gcagttcttt tagagcctta
    10741 cttcaaagta gaagttgtta ctccagaaga ttacatggga gatgttatgg gtgacttaaa
    10801 ctcaagaaga ggattaatcc aaggtatgga agctagaagt ggtgcacaag ttataaatgc
    10861 attcgttcca ctttcagaaa tgtttggata ctcaacagac ctaagatctt caactcaagg
    10921 tcgtgcaact tacacaatga tattcgatca ctatgagcaa gttccagctt cagttgctaa
    10981 gaaaatagct gaaggaaaat aatttaaaaa taattaataa tataaatata aaaaataatc
    11041 agctagatga gatatatctc atctagcaca agat
//
LOCUS       DACTGW010000129        10984 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.129, whole genome shotgun sequence.
ACCESSION   DACTGW010000129 DACTGW010000000
VERSION     DACTGW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.129"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            226..1401
                     /locus_tag="I9573_002475"
     CDS             226..1401
                     /locus_tag="I9573_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4797085.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQAYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            1436..2974
                     /locus_tag="I9573_002476"
     CDS             1436..2974
                     /locus_tag="I9573_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HAT4797086.1"
                     /translation="MQGALKENKKKSGGGFLGWIERVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQVI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            3006..4208
                     /locus_tag="I9573_002477"
     CDS             3006..4208
                     /locus_tag="I9573_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAT4797087.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFIQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     SEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     IWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(4387..4986)
                     /locus_tag="I9573_002478"
     CDS             complement(4387..4986)
                     /locus_tag="I9573_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT4797088.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            5155..6513
                     /locus_tag="I9573_002479"
     CDS             5155..6513
                     /locus_tag="I9573_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797089.1"
                     /translation="MSNIITNLIESVFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            6506..7138
                     /locus_tag="I9573_002480"
     CDS             6506..7138
                     /locus_tag="I9573_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAT4797090.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSKIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            7467..8624
                     /locus_tag="I9573_002481"
     CDS             7467..8624
                     /locus_tag="I9573_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT4797091.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     FNSEEIVYFTPKGKSYHSIKNCSGLSRSKKILSGTIEESKKNDPCDICYGK"
     gene            complement(8772..9407)
                     /gene="lexA"
                     /locus_tag="I9573_002482"
     CDS             complement(8772..9407)
                     /gene="lexA"
                     /locus_tag="I9573_002482"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAT4797092.1"
                     /translation="MYLDLTEKQVLILEFIKSQITLKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(9511..10638)
                     /locus_tag="I9573_002483"
     CDS             complement(9511..10638)
                     /locus_tag="I9573_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAT4797093.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
BASE COUNT         4076 a         1356 c         1781 g         3771 t
ORIGIN      
        1 aatttactaa aaataaaaaa actcattata ttaagatgaa aatagaaaaa taactagaaa
       61 aatactataa tatatcagct taatacagaa aaaagttaaa aatattgaga aaaataaaaa
      121 tgaaaacgtt ttatattgac atagagaaaa agtagtgata ccatttaaat atagtttagt
      181 agctaaacta tatttaaatt ataataacta ttttaggggg atgttatgaa tttaaaacaa
      241 ttaagtgaaa aatacgaaga atatgtaatt caaatgagaa gagaatttca taagtatcca
      301 gaattatctt ttaaagaaat aagaacaacc caaagaataa gggaagaatt acaaaaaata
      361 ggggtccctt ttgtatctat tgaaccaaat atcgtaatag ctactataca aggcaaaaat
      421 gaaggtaaga caattgcaat tagaggagat atagatgcgc ttccaatgca agaagattca
      481 gatgttgaat acaaatcaga atatgatgga ataatgcatt cttgtggaca tgatgctcat
      541 ggagctatgt tactaggact tgcacatatg ctaaatgata taagaaaaga tttaaaaggt
      601 agagtattat tagtttttca agcagctgaa gaagttggtg gcggacacaa ggagataata
      661 cagtacttta aggaaaatgg aggaccagat agattaatag ctactcatgt ttggtccgat
      721 attgaagctg gtaaaatttc tgttgaacca ggacctcgta tggctggtgg gtctggttgg
      781 agaatagaca taacaggtag aggaggtcat ggttctagac cagatcaaag catagatcct
      841 ataaagccag cttgtgatat agttttaaaa gtatcttcta taccagttaa tcatataagt
      901 gtactagaac aatgtgttgt acatgcatgt gcaatacatg gaggaactac aataggaaat
      961 gttattccaa acaatgcaga ggtaataggt ggatatagat attttactga agaatcaggc
     1021 aaaaaagtgt ttgacattat aaagcaaatg agtaatggtg ttggacaagc ttatggagtg
     1081 gatgtgaagg ttactcatac tggaggcata ggacctgtta tcaatgatga agaagcagtt
     1141 aagatggcta aagaaatagt ttctaatata gatggattgg agttagattc ttatgagcca
     1201 atatgtgcat cagattgtta tggtgaaata ttaaaagagt attctggttt ttattgttat
     1261 ctaggggtag gtaacaaaga aaaaggaata atatatgcac aacaccatcc taagtacaat
     1321 atagatgaag atacattaaa gttaggttgc gaatttatgg ctaaatatgc agttgaattt
     1381 ttaaataaaa ataataacta aaataaatcg aattttataa atggggggga tgaaaatgca
     1441 aggtgcatta aaggaaaata agaaaaaaag tggtggagga tttttaggat ggattgaaag
     1501 agttgggaat aaaatgccac atcctctagc attatttaca tatattacat tgatagtagt
     1561 agcattatct gcaatagcaa gctttttagg tttttcagca acaagtcctg ctacaggaaa
     1621 gttaataaaa gttatgaact taataagtgc aaaaggttta gtactattta tgcaagagtt
     1681 tgttaagaac ttccaaaact tccctgtgct tggggtagtt ttagttatgg cagttgccac
     1741 aggagtttgt gaaaagacag gatttttttc aactgccata aaaatgagtt tatcaaaagt
     1801 taaggggaat gcggttgtat ttataatagc ttttatagga gtatttgcta atcaagctgg
     1861 tgatgctgct tttgtattag taccaactct tgcaggagca atattttatg gattaaatag
     1921 aaatccatta gctggtatat tctgtggatt tgcatcagtt ggtggtggat ttgcaacagc
     1981 tgtaattcct gggggatggg acgttacgct aacgccaata actgtatcag ctgctcaaac
     2041 tattcaacct gcatttgata tgacattatt agctagttat tatgcactat ttgtatctgc
     2101 gtttatagta gctacagtat caacaatagt aacggttaaa tttatagaac ctaaattagg
     2161 taagtatact ggtaaaccag aaggtataga tgataatcaa ggttttgagg tcacaaaaga
     2221 acaagcaaaa gcagcaaagt tagcaggttt tacagtttta gcttatgtag cgttattaat
     2281 tgctttgtgt ataccagcta atagtttttt aagaagtcca gaaggttctt taatatttgg
     2341 agcaccactt atgtcttgct tacagttttt tatagtaata ctattcttct tgccagggat
     2401 tgtgtatgga atgagggttg gtaaaatcaa gactgtaaaa gacttaaatg atatattatg
     2461 tcaatcaatg gcagcaatgg caccgtttat cgtacttgca atagttatag gtcaattctt
     2521 aacgctattc tcagtgtcta atttagcaca ggtactagct ataaaaggtg gagcattact
     2581 caactcattg ccactaccac cacaagttat aatactgcta ttcttagtac tagtagcgtt
     2641 tataaatata tttgttataa gcggaagtac aaaatggatg atatttggac cagtatttgt
     2701 tccgatgtta atgcaattaa acatacatcc agcatttact cagtttgtat atagattagg
     2761 agattctgta actaatcatt tatcaccatt aaatgcattc tttataatac ttttagcaac
     2821 agtccaaaaa tacgataaaa atgcaggtat gggaactata ttctcagcta tgatacctta
     2881 cactataggt tactttgtag tacttgcagt tttagtagta gtttggatga caataggtat
     2941 accagtagga ataggtggac aagtttggtt ataaaacata tttaataatt ttgggaggaa
     3001 agaaaatgga aacaactgaa ataagataca attttgatga agtaatagat agaagtggaa
     3061 caaactgtct taaatatgat cttaagagtc aatttatgcc agaatctcca gaagatgcac
     3121 taccactatg ggtagcagac atggattttg catgtgctcc tgagataatt aatgcaatgc
     3181 atgaaagaat agacagaaaa atatttggat atagctctca acaaactgat gattattatg
     3241 aagctgtatg tggatggttt aagagaagat ttgattggaa aatagaaaaa gagaatatat
     3301 tctttagccc aggggttgtg cctgcgttag gatttttcat acaaatatta acacaaaagg
     3361 gtgatggtat cataatagaa aagcctgtat actatccttt tgaagataaa ataactaata
     3421 atgaaagaaa agtagttaat agtcctttaa agtttgaaga tggtcattat tctatggatt
     3481 atgatggatt tgaagaatta gctaaagatg aaaataataa ggtgtttata ttatgtagtc
     3541 ctcataatcc agttggtaga gtatggaaaa aagaagaact tagaaaaata gtagacatat
     3601 gtaagaaata tgatttgtgg ataatatctg atgaaataca ttgtgatatt attcgtaaaa
     3661 gtgaaaagca tataccatta gaagtagcat gtcctgatta taaagataga ataataactt
     3721 gtacagctcc aagtaagagt tttaacttag ctggtatgca attatcaaat atagtaatca
     3781 ataataagga attacaagaa aaatggatgc ttgaaacaga ttctaaatta gctatattcc
     3841 tacctaatcc attcgcaata gttgctacta aagttgctta taatgattgt gaagattgga
     3901 tgaatcaagt aaatgagtat atagatggta atattaaata tgtagaagat tttgttaatg
     3961 agtatatgcc taaagtaaag ataataaaat ctcaaggaac ttacttaatt tggttagact
     4021 ttagagggta tggattagat gcaaaacagc ttgaatatgt aatgcttaac aaggcgaagg
     4081 tattgctaga tgaaggatac atattcggag aagaaggagc aggatttgaa agaataaatg
     4141 ttgcttcgcc tagaacaatt atagaagagt gtatgaatag aataaaatgt gcatttgaag
     4201 gattataata gtaatatcta aataatatat ttgtgtattt taaaaaaaga ctatgttaaa
     4261 aaaatatttc aaagcatagt cttttaaagt tttatgatga gtttaaaaaa tttcaaaacg
     4321 ttactattat tgataatctt cacttatatg tttatattag aaataattcc acaatatgct
     4381 cattttctaa agttgcactt ttatacacct tgcttcacca atatcatgtg taaccaaaat
     4441 agtagactta ttctcattct taataatact ataaacatca tcacaaacta ataatctagt
     4501 ctgataatca agagctgaaa aaggttcatc taaaagtaga atatctggat ttacagaaag
     4561 tgttctaata agcgcaactc tttgtctcat acctccagat aattcttttg gatacatact
     4621 tctaaaatcc cataatccat aagtctttaa aaggctttca actctatcta aagatttttt
     4681 atcttttttg ccttgaattt ccaatccaat agttatatta tccattatat ttctccattc
     4741 aagcaaatta tctttttgaa acatatatcc aatatttcca gtaatcttta tatcaccact
     4801 agtcggtttt aaaagattag taagaatatt taatattgta gatttaccac ttccagaagg
     4861 accaagcaaa gttaatattt ctccttcttt caagttaaaa ttgacatcct ttaatacttc
     4921 aagctcgcct tccttagtgt agaagttcat atttatattt ttaacttcta ctatgttgtc
     4981 attcattatg ccacccccta taacttttag ctcgtatata ttatgttatt aacaatatga
     5041 cctatttgta ccatttttca taaatataat ataaatattt tgtttattta cgtattttct
     5101 tagtaacttg tacgtataaa ttataataaa taaattataa ggtaggggat aaatttgagt
     5161 aacataataa cgaatttaat agagtcagta tttactaatc caataactat attgttttta
     5221 cttgcaataa ctgtgtattc tgtttttaaa ataatagaaa ataacagagt atattcattt
     5281 gtaagttcaa aattagacga aatcaataga gattataaga caagtgagtt ttataaaaag
     5341 gtaagggatg actactatac atatagtaaa gaaaatcctt atgcagacgt aaatataact
     5401 tcatttatag aggaagttgt atctgattta aagcataata atttgccatt gttagaaaaa
     5461 attagaagta taaaaaattc atcttcaata agtatattac ttggggtttt aggtacgttt
     5521 gttggtcttt ctacaatgct tttatgtgtt gatactaaag acattataaa ttcacttcct
     5581 tcaactatta gtagtatgca aactgcattt acaactagta tatttggagt tgtattttct
     5641 ctaattatag gttactttac taagataaaa gattgtgaac atgttttgat acaaataatg
     5701 ttaaaatctg aaaatttact aacatctgaa ataactcatt ttaagtcaga aagaatggat
     5761 ttaaaagtag aagaggtaaa aaatacaata aaacaaataa gtaagtctat agaagctatt
     5821 gaaaaatttg ataaaatatc taaagattta aatgacttta atgatgaatt tatcagtggt
     5881 atagaagctt taaaaagcct tttagaaggt tctcaaagtt ctattaagac atttgaccag
     5941 agcgtaagaa aattagataa acagtttaat attttaaata taaaatttgt aaagcttttt
     6001 gataaatatg ataatcaaga taatataaat aaagagattt tatttgatat aaaagaatct
     6061 tcaaaaaata tatacaatgc cactgaaagc caattcaaaa ttagagatta tataaaaaat
     6121 ataaatgcag gatttgctct atatgaaaga agtgctcaag atttattaac taaattaatg
     6181 acccatgaaa ataaaatctc tcaaaatcaa aaaatccttt tagatgaaaa gtttacctta
     6241 gatgactcaa taaaaaatct ctcaagtatt attgaaaact tttctaatga cctacaagtt
     6301 aaactggata tgatgtttga aaattcttta gatattcaag ataagttaga tgttatgttt
     6361 aataactctt ttatgaatga tgaagttcct cttgatagtg aggaattatt taaggatgat
     6421 gttaataatg tatttaatcc ttttagtgaa gaaatttatg agatagagga taaagaaata
     6481 aaggtcatag gtgaggatga gttaaatgaa taataaatat agaagaactg ttaacagaga
     6541 atttgaaaag agtagctttt ggccaacatt tacagattta ttatctactg tattgatggt
     6601 tgtgattctt atcttattta gctcagaaag tatttcaggt tctgttgaac aagatttagc
     6661 taagaatgtc aatgcttctg ttgaagaaac ttttaaaaaa agcaaaatac ctgtgaaggt
     6721 agataaatct aatgggcaag taacttttgg tgaaaaaacc atgtttgatg tagatagtga
     6781 tgttttaaaa ccagaggcta aagaaatgct aaaaatgttt gttccaaagt acatagagac
     6841 tatttataaa gattatggtg actatatatc caaaatagtt atagaaggtc atacagatga
     6901 tgtgggaagt tatatataca acttagattt atcgcaaaga agagcatata gcgttgctaa
     6961 atttatagtg ggagatgaaa taggtgacta taaatacaaa gataaagtga ctaagcatat
     7021 tattgcaata ggaaggtcaa aagctgaact catcaaaaat ggtgacaata gcgtgaatag
     7081 agatgcatct agaagagtag aactaaaata cgaaataaat attaatcaaa ataaataatt
     7141 aaatttttat aaaaaaaaag aaggcagttg gtgtgagctt taagccttct ttaaggaaaa
     7201 agtttattta tttttgttga tacttaacat attatagttt ttagaagcag tttgcaatat
     7261 attaaacgta aatggtcgtt taaaatgcat aactggatac tactgatgta aaatatttca
     7321 ttttatataa tataaaaatt atacagtgtc aattttctat attattgtac tataatctat
     7381 tatttaaaat attatgtaaa aaaatatcat aaaaacgcat atcatgttat aattactaga
     7441 gtattatgaa tatggaggcg tagtttgtga tagaaataag tataaaaaat aaaaaaaatt
     7501 ctaaaattaa aactttgata cttttattga tagtatctat atcgatttat ttaactggtt
     7561 gtactacatc agataatgga aataatcaat caaataataa tgattctttg tctagtagta
     7621 tatcaagtga taagaatgta aaaattcatt ttattgacac tggaaatagt gatgcaatat
     7681 tgattcaaga tggtaaaact tttacattga tagatggggg agataatgat gatgagggat
     7741 taatggtaga ttacctaaac aatcaaggtg taaaagatat taaatatctt atagctaccc
     7801 attctcatgc agaccattta ggtggacttg attctgttgt caaaaacttt aatatagaaa
     7861 atgtttttgt atcgaatggt tcagcagaaa caaagagtta tagggacttt ataaatgctt
     7921 tagcaaataa gggtttatcc ccaagtgtac cgcttgaaaa taataagttc tatttagaag
     7981 attcttattt tgaagttcta aatacaaatg ggggagatac tactaatgaa caatctctag
     8041 tccttgtata tactaatggg aatgataaag ttttatttac aggtgatgct gaagaaggta
     8101 ccgaaaaaga aatactacct aagcttgaga aagtagacct tttaaaagta gctcatcatg
     8161 gttctcgtag tagttcatct caagagtttt tagataaagt aaatcctgaa tatgcggttc
     8221 ttttagtagg caagggtaat tcatatggtc atccacatca ggagacaatg aataaattga
     8281 aaaaaatggg agtaaaagta catagaagtg atgagtgttc agatataata tttgaatcaa
     8341 caggtgatgg agtatttaca agttgtaaag atggaagtta taataaaggt gttagagaag
     8401 atggaaactc tggaaaatca aacagtaata caagtaaaaa tgattctttt aaaaataaac
     8461 aaaatacatt caattcagaa gaaatagtgt attttactcc aaaaggtaag agttatcact
     8521 ctataaaaaa ttgtagtggg ctttcaagaa gtaaaaaaat attatcaggc acaattgaag
     8581 aaagtaagaa aaatgaccca tgtgatatat gttatggaaa ataacaaaaa aacaaccaat
     8641 ttatctataa agcttctgaa ctttaaatca tttaaaaaat aaaataaatg cataaaaaaa
     8701 ataagctata atcaatagat aatacatttc atctatacga atagcttatt tttttaagta
     8761 ttttttaata gctatttaat aactctaaat acaccagtta caatacctac aatttcaact
     8821 tctgagtcat tgagcataat tggttccata aaatcatttt ctggctgaag tcttattaaa
     8881 tttccttctt taaaaaacct cttaactgta gcagattcac catttataag tgcaactact
     8941 atctgagaat ttaaggccgt attttttttg tctactacaa cgtaatcttc atctaaaatt
     9001 cctgcattaa tcatactttc gccttttact cttaatacaa aattgttact gccctttact
     9061 aaactggcag ggaatggaat gtattcctct atgttttgtt gagctaaaat gggttctcca
     9121 gctgttattt gacctactaa aggaagctct attatttctt gattagctcc agaaacatca
     9181 tttactttac tacgttctaa aacttcaata gctcttggtt tagtagggtc ctttcttatg
     9241 tatcccagtt tttcaagttt atttagatga gagtgcacag ttgaagtaga tcttaaccct
     9301 acagcagtac atatttctct tacagcaggt ggataacctt ttaatgtgat ttgagacttt
     9361 ataaattcca atatcaagac ttgcttttca gttagatcta aatacataac atcactcatt
     9421 tccaaacata ttttgttttt atttccatac actttatatt tttaattata acataatcca
     9481 tttatatctg caaacatttg ttcctgattt ttaatcgtcc tcaatccatt caaaattctt
     9541 gactatatca cgttttacat ttttcttatg agctgcataa agctgagtag ttgtgacatt
     9601 atcatggtca agtaagtttt gaacatcata tatagaaaat cctgtttgga ttagagaagt
     9661 tgcagccgtt gctcttaatt tatggggact atatccattg tctctagtag tatcaagtgg
     9721 aatagatgta tattttttta ccagtgttct tatagccttt gctgtaattc tcttattttg
     9781 aagagacaaa aataaagcat ctttttcttc atcatttaat agttcatctc ttgttctttc
     9841 gttttgcaaa taatctttta ttacatgttc acaagtatga tttataggca ttaaaacttc
     9901 tttcccacgt tttctatata ttttaaattc acctcttgaa aaattaaaag aggagatatt
     9961 gagttctcta agctcattta atctaagccc gtatgtaaca aataatgcta gtatagcttt
    10021 gtcacgtaat ttagtttttc tccaatatac cttttctttc tctgtaagcc cttcacctgt
    10081 ttctactgat tcaagcatct tagcaacttc atctatctca aggcgcttaa tagcatctgg
    10141 ctgaggttta ggaagtttta ttggattgaa tccatcagtt atattattat ctatctgaga
    10201 gtttctgtat aagaatttaa atagagtaga gatggatgat ttttttcttg ctaacgctct
    10261 attgttattt tcaaaaatca atgtgttttt ttctgtattt ttgtaatatc tactgcaata
    10321 atcacctaag aataaattta tatctcttga ttttattgta ttaaaatcat cttctgtaat
    10381 atcttttaca caatctgcat ttgttaattc tttggtttca atcaaatatg aacaaaaaaa
    10441 attaatatct tctaaataag caagtcgtgt agaaactgca acagaacctt ttaaatatat
    10501 aaaataatcc ttcataaata caggaagttt attttccaaa agtagacatt tttctatgag
    10561 ttcattttct ttgataacaa tattatcagg tttatctgat ttattatgta cttttataat
    10621 tttcttatca ttattcaaaa ttaaacctcc taaaactaaa atataaatct tttatattat
    10681 tttttgatat ttataccaag tgagtataaa tatttaattt aatcattttt tacattttta
    10741 agagattagc agtgctttag atgaaattat cactaactat tccctaaatc cacatttaaa
    10801 gactagttga atttaagtac tcttttttta tttctcaata tatttataga aatattttaa
    10861 aatatattga gaaatttttt atgctctact aaaatctttt ttatataata tttttatata
    10921 tttctaacga aaccaaatat tagtaaaata tgtatttatt taactaaagt ttttaaatgg
    10981 gctg
//
LOCUS       DACTGW010000130        10947 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.130, whole genome shotgun sequence.
ACCESSION   DACTGW010000130 DACTGW010000000
VERSION     DACTGW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.130"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            391..2490
                     /gene="nrdE"
                     /locus_tag="I9573_002484"
     CDS             391..2490
                     /gene="nrdE"
                     /locus_tag="I9573_002484"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAT4797094.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYLKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2503..3474
                     /gene="nrdF"
                     /locus_tag="I9573_002485"
     CDS             2503..3474
                     /gene="nrdF"
                     /locus_tag="I9573_002485"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAT4797095.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKKMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3550..4176
                     /locus_tag="I9573_002486"
     CDS             3550..4176
                     /locus_tag="I9573_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797096.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIIEIPEQKFIMLKGSGDPNSKEFI
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            4571..5200
                     /locus_tag="I9573_002487"
     CDS             4571..5200
                     /locus_tag="I9573_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797097.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            5201..5983
                     /locus_tag="I9573_002488"
     CDS             5201..5983
                     /locus_tag="I9573_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797098.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAINTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            5976..6854
                     /locus_tag="I9573_002489"
     CDS             5976..6854
                     /locus_tag="I9573_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797099.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIIGKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIKIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSEYYI
                     KLKKDILMTMKSNKDI"
     gene            6887..7846
                     /locus_tag="I9573_002490"
     CDS             6887..7846
                     /locus_tag="I9573_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAT4797100.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDVALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            8160..8852
                     /locus_tag="I9573_002491"
     CDS             8160..8852
                     /locus_tag="I9573_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4797101.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            8862..9866
                     /locus_tag="I9573_002492"
     CDS             8862..9866
                     /locus_tag="I9573_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4797102.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGIHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKM
                     "
     gene            10114..>10947
                     /locus_tag="I9573_002493"
     CDS             10114..>10947
                     /locus_tag="I9573_002493"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017352249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAT4797103.1"
                     /translation="MSLDVIFILKSVIIAIVEGFTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FT"
BASE COUNT         4336 a         1043 c         1988 g         3580 t
ORIGIN      
        1 aaataatata taaaatatca tcttgaaagt acttttttca atatatcaga atatattttt
       61 aagctattta gagataattt aaatttttaa acaggacata ataagcttgt atttttttat
      121 atttgagttg ataaaaattt ctttaaataa aaataatgtc aattataaag taataaaagt
      181 ttagatataa aaaaatttgt gtatatacta tatgtagtat ataatagata aagcactgac
      241 tatatgtagt gcttttgttt ttgaaaaata cttattgcat ttctataatt atagattaat
      301 tttgggattt caactgataa gtaccaaaag aaataataag caaattaagt gaaattatgg
      361 cacaagatta tgttaaaagg acggtaaata atgaattgga tagagcttaa caatcaagtg
      421 ataattaaaa atgaacaagg taagtaccaa ttggaaaagg ataaggaagc cttaagtagt
      481 tacatagaag aatttataca accaaagttg agaaaattta ataacctaga agaaagattg
      541 aagtatttaa tagatgaagg ttattattta aaagaagtaa taaatcagta tagcatagat
      601 gttattaaaa atatatatga tatgattgat aaacataagt ttgaatttca atcatatatg
      661 agtgcaaata agttttatca aaattatgca ttaaagagca atgatggaaa ggaaatatta
      721 gaaacatata atgataaagt gttaattgta gctttgacac taggaaatgg cgataaagat
      781 ttagctttaa acttggcaga taaaatagta aaacaagaat ttcaaccagc tacacctact
      841 tttttaaatg ctggaagaaa gagggctgga gaaatggtat catgtttttt actatctgta
      901 gaagatagta cagaaggtat atcatatgct atatcttcat caaatcactt atcaaagatt
      961 ggtggaggag ttgcactaaa tttatctaaa cttagagcat ctggcgaatc tatcaaagat
     1021 atagaaggtg cagctggagg tgttgttgga gtagctaaaa tgttagaaca atcatttagt
     1081 tactttaatc aaatgggagc tagacaaggt tctggtgcgg tgtaccttac tgtattccat
     1141 cctgattttg aattgttgat ggatactaaa aagataaatg cagatgaaaa aataagactt
     1201 gctacattat cattaggagc tataatacca gataaattta tggagttggc agaaaaaaat
     1261 gaagtagcat atgcatttta tcctcatact gtgtataaga aatatggagt ttctcttgat
     1321 gagatagaaa tggatgagtg gtatgataaa ttggttaatg atagtgatat aagaaaaaaa
     1381 gaaataaatc ctaggcagat gttgacaaaa atagcacaaa tgcaacaaga aagtggatat
     1441 ccatatgtag tgtacataga tacagctaat agagaacata cattaaaaga tgttggaatt
     1501 attaaaatgt ctaacttatg ttgtgagata tttcaatatc aaacaccttc agaaatagag
     1561 gggtatggag gaaaaaatga atggggacaa gatataagtt gtaacctagg ttctttaaac
     1621 atagccaatg taatggataa taaaactata gaaagtacag ttgaaacagc aataagagca
     1681 ctgtcatttg tagcagatag cacagatata aaaccagtgc caacggtaag caatagtaac
     1741 agcaagtctc attctatagg attaggagct atgaatttac atggatactt agtaagagaa
     1801 aatattttat atacttcaga tgatgctata gatttttcta atgtattttt tgcaatggtt
     1861 agatactatt caattaaagc ttctatgaaa atagccatag aaagaaatca aacttttgaa
     1921 ggatttgata aatcagaata tgttaaggga agaaatagca aggttttatc aaaatactat
     1981 gaacaatctt atctacctaa gtcagaaaag gtaagagcat tatttgaagg gatttatata
     2041 ccaacaaaag aagattggac aaaattattg gatgaagtaa aagaaaaagg gatatataat
     2101 gcatatttaa tggcagtagc accaactcaa agcattagtt atgttcaaaa tgctacatca
     2161 agtataatgc caattactga gccagtagaa gtaagaacat atggagactc tactacaata
     2221 tatccaatgc catttttaac aaatgataat atgttgtatt atcaatcagc atacaggatg
     2281 gatatgagaa aggtcataga tttagtggca acagttcaaa accatgttga ccaaggtata
     2341 tcaacaacac tgtttgttac agatgaaaag acaactagag atatagctag acattacata
     2401 tatgcctata aaaaaggttt gaaaagttta tattacacaa gaacaaagat gacaagagat
     2461 actcatgagt gtctagtgtg ttcagtatag ggagagaaat atatgacatt taatttacat
     2521 aaaatacata atgcagttaa ctggaataga gaagaagatg gatttacaca agctttttgg
     2581 gaacaaaatg taaaacaatt ttggcttcct gaagaaatat ctgtttctaa agacataaag
     2641 gtatggaatg aactaagtaa taaagaaaag gaattatata aaaaagtatt gggtggttta
     2701 acattactgg atacaaaaca ggggaataat ggaataccat ctatgatgag tttaacagaa
     2761 aacctacaaa gaaaagctgt cttgtctttt atggggacta tggaagaaat acatgcaaaa
     2821 agttattctt ctatatttat gactctactt tctaatttag aaatagatga attatttgaa
     2881 tggatagaaa cagaaccaac attacaaaga aaggctgatt tagttttagc tcaatacgaa
     2941 aatactacaa atcaagaggg attataccta tcaatggtta caagtgtatt tttagagagt
     3001 ttcttgtttt attcaggatt cttttatcca ttgtatctat ctggtcaagg aaagatggta
     3061 gctagtggag aaataatatc gcttattctt agagatgagt ctttacatgg taagtatata
     3121 ggattgctag cacaagaaat atacgattca tttgataaaa tggataagaa gatgttagaa
     3181 gagaagatgt attctatact ttatagcctt atggagaatg agatagagta tacaaatgtt
     3241 atatacagag aaagtgggct tgaaaaagag gttgtgaact ttttaaaata caatgcgaat
     3301 agagctttag aaaatctagg gtttgagaag ctatatactg tagatgctat aaatccaatt
     3361 gtattaaatg gtcttagcac tgaaaccaaa acacatgatt tcttttcaac taagggtaat
     3421 ggataccaaa aaggagtgta tgaggaatta gaggatgaag attttataat ataatttata
     3481 attatgacac actgttgtca cttttagcgt gataatatca atgtataaaa tatatatgga
     3541 ggtattttta tgaaatatga atggagaaaa agagataaag agttatattt acctaaaaag
     3601 aatcctgtaa taattgaaat accagaacag aaatttatca tgttaaaagg gagtggagac
     3661 ccaaattcta aagaatttat tgaagcaata ggagtacttt attctttggc atatgcaata
     3721 aaaaccatgc ctaaaaaagg aattgtacca gaagggtatt ttgactatac ggtttttcct
     3781 ttagaaggta tatgggataa gggagaaaaa agtaaaggct tagatgtact tattaaagaa
     3841 gatttaattt acactataat gatacgtcag ccagattttg ttacacaaga cctgttggat
     3901 aaagctactg atatagtaaa gaagaagaaa cctcataatc ttcttgaaaa agttaggttt
     3961 gggtctatta atgaagggct atgtgttcaa atgcttcata taggttcata tgataatgaa
     4021 ccagaaagtt ttgacattat gaacgaattt tgcaaaaaaa ataaacttat aagaaaatct
     4081 tacacacatc gtgaaattta tataagtgat gctagaaaag tatcgcctga atcattgaag
     4141 acggttttac gttttgaagt agaaagggaa gtataaatat tttgaataat ttataagagt
     4201 tataaaaagt gaagtctgtt gagacttcat tttttgatgc aattttaaaa ttaaatttga
     4261 tgtttcataa tattttaaaa actttctact tttattagtg tgaataactg ttgctattaa
     4321 ataataatac taattatata agtattatta tataatattt atagattatt atataaatag
     4381 attattagta agatgtatgc attattaatt aaaataaatt acatatacta tttaatattt
     4441 catttaaaat attaaaaaat gtagataaaa attgttttat taaggaaaaa ttaatatatt
     4501 gacaaaattt aagtgctcat ttaaactatt aattgataat aatattcaat attaaataat
     4561 aggaggcgtt atgaataaat atcttctaat aaatccagtg gcagaaagaa tgtatggaga
     4621 aaagtttcac cttattaaag caaatttgat aaaaaagggg tacataatag cagaatgtga
     4681 accacagtta gaatatgtaa aaaagcagta caaagagtat acaaaaaaga ctgaaaatac
     4741 aatgttggat tgtagatgtc cagaaagtat aagtctttta aaaagaaata atttgataaa
     4801 taattttgaa gtacctatga tagaaccaat attagtaagg acttgtaggg ttttatacga
     4861 taaatatata aaaagtaaag atgatatgtt ggtagtaacc tgtccatgta cacaattaag
     4921 agactttgca agtgaaaagt ttaaggataa gggtaatgct atgttttata catggaaaga
     4981 gtttgctgaa caagaaggtg taaaatcact tggaaaaata gatgaatcac caattccatt
     5041 aggatatttc aaagatactt ttgaaaaagt tcttgaagta tcaagtgaag acgaaatatt
     5101 aagtgaaata caaaaaataa gaaatggctt tgaagataag tatgacatag tagaaatgtt
     5161 ttattgtaaa gatggttgta ataatggaga tggtttgtaa atggtcaata aatttaataa
     5221 taagttttta aggctagtaa agagtgttct gatatttttt ataattcttt tattatggaa
     5281 aattacaaat tatcttggta tttggagtga ttatatatta ccatcacccg aaaaggtata
     5341 tagtacattt ttaaatatga taagtgatgg ttcaatattt attaatgtat atgcaagtat
     5401 gaaaagagtt ttgataggtt ttgcaataaa tactgctata ggtgtgcctt tagggatatt
     5461 ctttggaatt tatagtggag tatacgaata ctttaaatct ctaataaatt ttttaaggaa
     5521 tacacctccc cttgctttaa taccaatgct tatattatgg tttggtatag gagaggaatc
     5581 aaaaattata attatagttt tagcatcctt tttcccaatc tttacaagca cattgaaagg
     5641 gattaaaaat tgtgactcaa aactgattga agtgggtaga gtatttgagt tttcaaagct
     5701 tcagataata tttaaaataa taataccaaa tgccattctt gatattgctg ttgggttaaa
     5761 gttagctctt ggatatagct ttagagcaat tattggggca gaacttgtag cagcttcatc
     5821 tggtcttgga tacttgatat ctgatggaaa agaaatgtct agaacagatg ttgttattgt
     5881 aggtataatc gtaattggac ttttaggaat aattacagat tacatatttt cgattattgt
     5941 gaagaaggta agtaagggaa agatggtgga agcatatgaa taatttagta tgtgactctg
     6001 gatatataat tagaaattta tataaaagtt ataaagttga caatgaagaa catgtagttc
     6061 ttagtgatat ctctttaaac atagatagaa accatataac agtaatttta ggtgaaagtg
     6121 gttgtggaaa gactaccttg cttagagcaa ttgcatcact tgaaggtata aatagtggtg
     6181 agattaggtt tattaatgat aataaagagt atagacctaa tgttggtttt gtttttcagg
     6241 agagcagatt aatgccttgg ttaaacgtaa gtgaaaatat agcttttcat aatcaaagaa
     6301 aaaatactat tttaaacaag gtgcttagaa aatttaaaca caataaaaga attattggta
     6361 aaattataaa tgataatttt agaagcttag aaacaaatga taatttgaac aaatcaaata
     6421 aatataatag gaaaagtaat attgatgtag aaaaatatct taagatgatg aacttgcaga
     6481 aatttaaaaa ctcttatcca aatgaattgt caggaggtat ggcacagaga gtaagcatag
     6541 ctagagctct ttctttcaat ccagatatgt tgcttatgga cgagcctttt tcggcattag
     6601 attattttac aaggattgat atgcaaaatg aagttataaa aatacataag tcaactaata
     6661 aaggcgtaat atttgtaact catgatattg atgaagcgtt aaagataggc aaaaaaatag
     6721 ttgttttcac agacgaaaga aaaataaaag aatttaatat agaagatggt tataatagag
     6781 atttaacttc agagtactat attaaattaa aaaaagatat tttaatgaca atgaaatcaa
     6841 ataaagacat ttaaagaaat taataatatt ttaaggagta gagtaaatga aattaaaaaa
     6901 gctattgata ggtataacga tattaacact tgcaacagca tctttagctg gatgtgctaa
     6961 aaaaaataat ggagaaaaac tttcagaaat aaatttaact tatgtaaaat caccattaaa
     7021 tgttccttct ataattcaga aacaggatga tttatttgga aaggaattta aaaaggataa
     7081 tataaaagtt aactttcacg aaattactac aggtccagag caaacacagg ctttagctgc
     7141 tggagaaata gacttcttac atgcactagg tggtacgtca gctttaatag ctgcatctaa
     7201 tggagtagag ttaaagatat taaacactta tagcaggtca ccaaaaggat ttatgatttt
     7261 gactaataat aattcaataa aatcagcagc agatttagta ggtaagaagg tggcaggtcc
     7321 aaaagggact atattacatc aggtattgat ttcagcactt gataaagaag gattaagtat
     7381 ggatgatgtg gaatttgtta atatgggtat tccagaagca tcagcagctc tttctgatgg
     7441 tagcgtagat gttgcattga ttgctggtcc agcagctctt aaagctatga agtcaggtag
     7501 taaacttgta gcaaatggtg aaggtttagt agatggtata atagtaactg ctgttagcac
     7561 agactttgca gagaaacacc cagaaatagt tgaaagattt atgaaggttg aaaaggaaac
     7621 tttagaatat gtaaataata attttgatga agctatggaa aaagtagcta aagaagttga
     7681 tttaagtctt gaagaaacta aagaattata tgcttggtat gattttagtt tagatataac
     7741 agataaagat atatcttcgc ttgaagatac acaagacttc ttaataaaaa ataagttaca
     7801 agagaaaaaa gtgaatataa aagaattaat ctataatgct aagtagaaac tatgctaaaa
     7861 taatactaag taagaaatat aataaaagtt taagttacct tattaaggta actttttcta
     7921 ttttgaatta gtaatttcaa tttattttag aatgattagt tgataataat gattaattaa
     7981 tcataaaaag atactttttt atagagtttt attaagatgt ctaataatat ggttatactt
     8041 atgaagtcag taattgtgaa gttaataata tgattaaatt tatatattgt aaaaagatta
     8101 taatatatta aaattggact gttatatgat gagtaaatag gtatattagg aggaattata
     8161 tgagttataa agtatatatt gtggaagatg atatatcaat aagtacatta cttaaagatt
     8221 atattgagaa atatggattt gaagtgattg ttgaagagaa ctttgatgat ataatgatta
     8281 catttgaaag ttttaagcca cacttagttc tattagatgt aaatttacct aaatttgatg
     8341 gtttctactg gtgtagatgt attagacaac agtcgaattt acctataatt tttatatcag
     8401 ctagagatgg caatgtagac caaattagag ctttagaaag tggagcagat gattatataa
     8461 caaaaccttt ttactatgat gtagtaatgg caaaaataaa gagtcatata agaagggtat
     8521 atggtgaata ttcacctaaa atagacgaaa gaatagtgga gctagatgga ttaaagtttt
     8581 atccagaaag gttagaacta gaattaaatg gtgaaaatct aattataact aaaaaagaag
     8641 gtatactgat tgaatgtctt atcaagagat acccaaaagt agtaagtaga gattatcttt
     8701 tagaaaagat atgggatgat attgagtttg tagaagaaaa tactttaaat gtaaatgtta
     8761 gcaggataag gaaaagattt gaggaattag gtatagataa tgctgttcag actgtccgag
     8821 ggttaggata taaattaaat aagaattggt aaggagaaag tatgaagtta tttttaaaag
     8881 attataaagg ttatattttt atatattata taggcatagt tatgacaatt atttactgta
     8941 atcttatgaa gtttattagt tttggagaga gtttatatat tcttttattt agttctttta
     9001 tattgttatg ttttttaata tataagtatt ataaaaatag agaagtatat aatctgtttg
     9061 aaaaaggaat acattctttg gaggagtcat ttttgtattt aggaagctca ttcttgggga
     9121 gaaatatatc agttatttta aaacaacaac ataaattgta tcaatcaatg atacaagagc
     9181 ataaaaagat acatagtgaa catttaactt tcatcaacca atggattcat cagatgaaaa
     9241 caccaatatc agttataagc cttcagcttc aagattatga gggagaagaa gtaactgaaa
     9301 atataagaaa agaaatagat agattaaata aagggttaga tatggctatg cattttgcaa
     9361 gggcagataa ttttcagaaa gactttatag ttgaaaaagc caaattaaat gaaattgtat
     9421 ctagtattgt aaatgaagaa aaaaggttat ttataaaaag tggaatgatt cctaagattc
     9481 aagtagacaa atttacttat gtttatacag atgtaaaatg gatgaaattt ataataggtc
     9541 aattaattac aaatggagtg aaatattcta aagattatag taactaccta actataaaat
     9601 ctgaagaaaa taataaatat gtaattttaa gtataataga tgaaggaatt ggaattgttt
     9661 ctaaagattt aaaaagggta tttgacccat tttttacagg agaaaatggt cgtaagtttg
     9721 gagaatctac aggtatgggt ctttatctag taaaaaaggt atgtgatgat ttaggtcata
     9781 aagtaagtat aaaatcagaa gtaggaaaag gtacagaagt ttcaatctac tttaataaag
     9841 atgtaaatag tttgataaag atgtaaatta tctgttacaa tagggttttt agttaattgt
     9901 acttttttat tatacaattt attaaatgaa atattcatag ttaaataatc aaaatttaag
     9961 agagtttatt tgcaaacctt acatagatgt aaggtttgtg taatgtttgc agatagttaa
    10021 attttctaat ctaaattaca attaatataa gaaataacat gagagaaata acataaaaaa
    10081 agaaattttg taaaaaagga aggatgttta ttgatgagtt tagatgttat ttttatattg
    10141 aaatcagtca taatagctat agtagagggg tttacagaat ttattccagt ttcatctaca
    10201 ggtcatatga ttttggttgg taatttaata gattttaagg ggcaatttgc tgaaatgttt
    10261 gaggttgtaa tacaacttgg ggctatatta gcagtagtag tactttattg gaaaaagata
    10321 aaagatagcg ttatagaatt ttttaaattt atatttactg gcggaaaaga aggaaagatt
    10381 ggttttagat ttggaatgaa tgtaattata ggttgtatac catttgcgat tataggagtt
    10441 ctattttatg ataatataaa atcattgttt aatttacaat ctgtaattat aggatttata
    10501 gtgggtggga ttttattatt agtagtagaa actttgttta gaaaaaagaa tcattcaaca
    10561 gataatatag ataaaattac tccaatacaa gctttaaagg ttgggacatt acaagttttg
    10621 tctgcatggc caggtatgtc aaggagtgca tctactatta tgggtggatg gatagcagga
    10681 cttaattctc ctacagcagc tgagttttct ttctttttag cagtaccagc tatggtggct
    10741 tcttcaggaa aagatttatt tgaatttgat tattcaataa tgacaccaac attgtggatt
    10801 gctttggtag ttgggtttat agtagctttt attgtatcaa taatagttat ggaaaaattc
    10861 gtgaattttt taaagaaaaa accaatgaga gtgtttgctg tatacagaat aatcatgggt
    10921 gtagtacttg cagtattagc ttttact
//
LOCUS       DACTGW010000131        10869 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.131, whole genome shotgun sequence.
ACCESSION   DACTGW010000131 DACTGW010000000
VERSION     DACTGW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.131"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..805
                     /locus_tag="I9573_002494"
     CDS             <1..805
                     /locus_tag="I9573_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT4797104.1"
                     /translation="SQIIKNFEESEDVYLFERYKGRLNGLTPAGERFYVNAEHIINEY
                     KNMMEDLREDSVQFKGKVRIGIPPLILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLN
                     RMLILQELDYAVLLHPHKIDKSVVTEHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQ
                     LLAIFNPTFMIHHKLMQKFTDENVKLKRYIMSGSWDFLLLSTTNSDFITILPSPVHDF
                     FENSKIIEKPFNHPITWKVILCRPKKERYSHVDQHVFKFIIDFFSKKNS"
     gene            complement(1020..1832)
                     /locus_tag="I9573_002495"
     CDS             complement(1020..1832)
                     /locus_tag="I9573_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797105.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVISLTIISKKFSSNT"
     gene            complement(1835..2794)
                     /locus_tag="I9573_002496"
     CDS             complement(1835..2794)
                     /locus_tag="I9573_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797106.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            complement(2900..4459)
                     /locus_tag="I9573_002497"
     CDS             complement(2900..4459)
                     /locus_tag="I9573_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT4797107.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKRKEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            4678..5655
                     /locus_tag="I9573_002498"
     CDS             4678..5655
                     /locus_tag="I9573_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797108.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMVIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPVLGKNKDKRLYSIPGQV
                     PNPIGIPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            5656..6627
                     /locus_tag="I9573_002499"
     CDS             5656..6627
                     /locus_tag="I9573_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAT4797109.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPDEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTRALMSAVPVQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            6779..7273
                     /locus_tag="I9573_002500"
     CDS             6779..7273
                     /locus_tag="I9573_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAT4797110.1"
                     /translation="MSTLVTLLGLLVCTKISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKLSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPRMPLSVSLALAAVISSTDLVAVKSITQGLNFPKNMMSILEGESLLNDDDRII
                     NIME"
     gene            7481..8362
                     /locus_tag="I9573_002501"
     CDS             7481..8362
                     /locus_tag="I9573_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAT4797111.1"
                     /translation="MNKGNCILGEILIMIIQQIYNNNVVLVLDENIKKELILTGCGIG
                     FQKKKGQEIDKSKIERTFVIQDESFLDKISKLASQVDEKFFEISTEIIAYAEENLNTK
                     LYEYIYVALTDHIAFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDV
                     KFPVDEAGFIAMHIVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRL
                     LTHLKFFAKRLVKKEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDE
                     LLYLTLHIKRVISVLNL"
     gene            8665..10737
                     /locus_tag="I9573_002502"
     CDS             8665..10737
                     /locus_tag="I9573_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAT4797112.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLSHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKLLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNCLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVSIL
                     RYDIEKIG"
BASE COUNT         4089 a         1313 c         1706 g         3761 t
ORIGIN      
        1 aagtcaaatt ataaaaaatt ttgaagaaag tgaagatgta tatttatttg aaaggtataa
       61 aggtaggtta aatgggttaa caccagcagg agaaagattt tatgtaaatg cagaacatat
      121 tataaatgaa tacaaaaata tgatggaaga tttaagagaa gattcagttc aatttaaagg
      181 aaaagttaga ataggaatac ctccacttat acttggaata gcattttcag atgtagttgc
      241 acagttagtt gcaaataatc cagatataga atttgatata gtagagaagg gtgcatatga
      301 cttaaataga atgcttatct tgcaggaact agattatgca gtactgttac atccacataa
      361 aattgataaa agtgtagtaa ctgaacatgt gcttcaagaa gatgaactta cagcattttt
      421 aaatataaat catccacttg ctaagaagga taagatagat tggaacgatt tgaatgagca
      481 attacttgca atatttaatc ctacttttat gatacatcat aagctaatgc aaaaatttac
      541 tgatgaaaat gttaagttaa aaagatatat aatgtctggt tcatgggatt ttttgttgct
      601 atcaaccaca aattctgatt ttataacaat tttaccatca ccagttcatg atttttttga
      661 aaatagcaaa attatagaaa agccttttaa tcatcctatt acatggaaag ttatactatg
      721 tagacctaaa aaagaacgtt atagccatgt agaccaacat gtatttaaat ttattataga
      781 tttctttagt aaaaagaact cttaaaaatg ttattaaaat aacaaataaa taaatcaatt
      841 tttattacaa taaactatta gtttttcatc tatatgtttg agagcaataa taggctttac
      901 ataacatggt agagtagtat ttaaagttat aatatacaaa atatgtattt tttaaacgaa
      961 aaataaaaac agtgtatatc tttagataat cactgttttt attttctata taaaatttgt
     1021 taagtattac tagaaaattt tttgcttatt atagtcaaac ttatgactgt taaaaatata
     1081 agtaatcctg gaaatacagc taaatataac ttgtcaaaaa tatgtctttg agcatcttga
     1141 agcatattac cccatgatgc aagaggtaat tggattccaa gacctaaaaa actaagagcg
     1201 gattcagtta agatagcatt agcaatattt aacgaaccag cgactacaat agtctctttt
     1261 aagtttaaaa ctatatgtct taatagtata tttctagtct tgataccagt agctttagca
     1321 gctaaacagt attcattttc ctttattttt aatgtttcgc cacgtacaat tcttgaaact
     1381 tccatccaac ttaagaatcc aataattagt ataatagtgg aaactttagg tgttagataa
     1441 gtatttaata caatcaaaac taagaaactt ggtatactca taagcatatc tactaatctc
     1501 atcaagaatt ggtctagctt acctccaata tatccactaa taacacctac agcagtacca
     1561 aatactacag agataagcat tgataaaata ccaacactaa gtgaaatcct tccaccataa
     1621 agagctctag agaagtaatc tctaccaagt tcgtctgtac caaagatatg ctttaagcta
     1681 ggttcttgaa gtttagaagt agtatcaatt gcattagagt ctatatttaa tataaaagaa
     1741 gctataatta atatgattat tatgctaaga gtaatacatg caaaagcaaa ggatttatct
     1801 ttttttaatt tttttaagat acttgtaaac acaactagaa tcctcctttg ctagatttta
     1861 ttttagggtc aattaataag tatagtatat cagcaactaa attacctaaa attaaaaata
     1921 gtgctgataa catagttatt gccataatta cagtataatc tcttgaaaaa attgaatcaa
     1981 tcattaatct tcccatacct ggccaagaga aaactacttc tgtaacaaat gcaccagtga
     2041 ctaatttttg tatactcatg cctaggattg taaccaatga tgtcagtgaa tttttcaaga
     2101 catgtttaaa tagtatgact ttttctgata atccttttgc tcttgctgta ataacatatt
     2161 gtttttttaa ttgttcattt acactagctt ctacataatt tgtaaaaata gcagtattat
     2221 atagacctaa agttattaca ggcaaaacta aatgttttac taaatctcca atactatcat
     2281 ttccaatagt atgcatacct cctgatggga aaatgttaag tttaactgaa aaaattgaaa
     2341 tgagtatcat acctatccaa aaagaaggga tagaaacacc tatataatta aaaacagatg
     2401 atactttact agttattgaa tttttattta cagctaaaaa tattccaaga ggtatagcta
     2461 ccaatattga aaatatcaaa gaagtaccca taagaataaa tgtatttccc attttttcca
     2521 taatttgttc tgtaacgggc atatggttta tataagaaat acctaagttt ccacttaaag
     2581 tgtttttcaa ccagtttaaa tatcttatat atactggttt atcaagcccc atattaaccc
     2641 ttatattttc aatatcctgt gaagacatta aaggatttat atatgcatct gctgggtcac
     2701 caggagctaa ttctaaaagt ccaaatgata atatagatat taagattaac ataggtatta
     2761 aaacttttac tctattcaat attaatttta acataagtaa attctcctaa ttaaaagaga
     2821 tgtctcgttt taaaataata aaacaatatt atttttagac atctcctttt ctaattaata
     2881 atataaaatt ttacatcaat tattctacat acagtttaga ccaatcttca aataatattg
     2941 caggagatgg tttagcatct tttattcctt ttagattttt ttgagctgcc attaaatttt
     3001 gttcataatc tatagtgtaa ataggtgcat ccttagatat atcctgttgt atttgtttgt
     3061 atatttcttt acgtttttcc ttgtttaact cagtaaatcc tttttcccat aaagcatcta
     3121 tttcagcatt agaatattta gtttggttgc aatattgacc agtttcaaac atacctctat
     3181 atgggtttgg ttctaatccc caagcataac catttacata catatcacat tcagtgctat
     3241 tactaaatag tatattaaag aatgccttac tttcataagg aactatttca gcttctatac
     3301 caattttttt caactcttgt tgagctacta aggcatagtt tttatgccca aatctacctg
     3361 tgttataacc tatttttaat ttatcaattt taacaccact tttatccaat aagtctttag
     3421 ccttatttgt atcttggtca tatccttcaa caccttcttc tgtatagaag tctgcttctg
     3481 gaactaatat agatttagca ggtacagaac cttcttttcc atatgctgat tctatcattt
     3541 catttctatt taaggcatat gataaggctt gtcttacttc tttgttagcc atgtttgata
     3601 tattttggtt aaatacaatg tagtttaatc tttcttcact ataagtataa gtttgtaagt
     3661 ttgagtttga ttttgccttt tcatatcctt cagcagatgt cttcataagt gaaatttcgc
     3721 cattatttaa agctgcttct tgagatgctt cattaggaat tatttttagc gctatagaat
     3781 ctgccttagg ttctccacca aaataatctg cattcttttc aaatactatg ctttcacctt
     3841 ttttccattc cttaaacttg aatgcaccag aaccaactgg attattattt ttttctgact
     3901 tagctatatt tgattctcct tcaaatacat gttttggtat tggagaaatt ttagatatac
     3961 catataagaa agattcactt gcagttggta aagtaatctt aactgttaaa tcatcaattt
     4021 tttctacttt aactggtttt tcacccacca ataagtattg tcttgatggt gaattttgtt
     4081 tttcgtctaa aactgtatta aatgtaaata agacatcttc agcagttatt ggttttccat
     4141 catgccattt taaattggat tttaatttta tagttaattc cttactatct ttaaaatcta
     4201 ctttttctgc taaataattt actaaattcc ctttttcata agtataaaga ggagcataaa
     4261 ctatatttga tgcaagagag tcttctttaa cgtttgcaaa agctccattg attatatctg
     4321 ggtcacttgc cataggtatt acaacagttc caccagattt gcttccacta gacttgtctt
     4381 tatcattgga agcacaggct gtaaatgtaa gcattaatga tgaaactaag attaatgatg
     4441 ccaatttttt aaatttcata ttcttataaa acctccataa aataatattt aacacatatt
     4501 tttcattgta taatagtaat cattattaaa tcaatatata tagaaattaa ctattatatt
     4561 attataatta atagaaaaaa ataataaaat gtaagaaaat gttgtaattt aaatttttat
     4621 aaaatacaat caaaaggtgt atgttatttt aagtaatttt gctgagagga gaaaataatg
     4681 agtaaagcac taattgaggt taaagattta aaagtttatt ttcatacaga taaagggatt
     4741 gtaaagagtg taaatgaagt aagttttaat ataaatgaag gtgaaactat tggaatagtt
     4801 ggagaatcag gatgtggtaa gtctgtaaca gccatgtctt tgatgaagtt acttccaaca
     4861 tcaaaaatag aaggtggaga aataatcttt cgaggcaaag acattttaaa gatgaatgaa
     4921 gatgaactta tggtaataag agggaatgag atatcaatga tttttcaaga accaatgaca
     4981 tctttaaacc cagcgttcac tgttggaagt caaatcattg aaggtattat gatacatcaa
     5041 gatttatcaa aggaagaagc taagaaaaag gtaatcgata tgataaaact ggtagaaata
     5101 ccaagagctg aagaaatata taattcatat ccacatgaat tatcaggtgg tatgagacaa
     5161 cgaataatga tagctatggc tttatcatgt aatccaaaat tacttatagc tgatgaacca
     5221 acaacagctt tagatgttac tattcaagct cagattttag atataatgaa aaatataaaa
     5281 gaaaaattaa acacatctat aatgatgatc acacatgatt tgggagtagt tgcagaaatg
     5341 tgtgacaaag tattagtgat gtatgctggt aaaattatag aagtggcaga ggttgttgaa
     5401 ttatttaaaa atccaaaaca tccttatact attggacttc taaaatcaaa gcctgttctt
     5461 ggaaaaaaca aagataaaag attgtattcg attccaggac aagtaccaaa tccaattgga
     5521 ataccagatt catgttattt tagtgataga tgtgaaaaag tatgtgataa atgtagaact
     5581 cagatacctc ctttaataga attgaatagt ggtcatagta ttgcatgttg gttatataaa
     5641 aaggaggaga tataattgaa aaaagaagag agtttattaa aagtagaagg tcttaaaaag
     5701 tactttgaag ttaaatctgg attatttgat aaggaaatta aatgtgtaaa ggcagtagat
     5761 ggaatttctt ttgatttaaa aaaaggagaa actttggcta tagttggaga atcgggatgt
     5821 ggaaaatcaa ctgcaggaag aaccatcctt agattaattg aaaaaactga aggtaaagtt
     5881 gaatttaatg gtgtagatat atataagtta gagaaggaag aacttagaaa gttaagaccc
     5941 aaaatgcaaa tgatatttca agacccatat agttcattaa atccaagatt aaatgtggaa
     6001 caaataatat cagaggcagt tatagaacat aatttagtgc caaagaatga aataaaagat
     6061 aatatattaa atgttgtaga aagttgtggt ctttctaagt accatttaaa tagaaaacca
     6121 gatgagtttt ctggaggtca aagacagagg ataggtatag caagagcatt agcattaaat
     6181 ccagaattta tagtttgtga tgaacctgtt tctgcactag atgtttctat tcaatctcaa
     6241 attataaatt tacttatgga tttacaggat aaatataaac tctcatattt atttatatct
     6301 catgatttga gtgtagtaaa tcatattgca catagagttt gtgttatgta tctaggttct
     6361 ataatagaaa ttgcaactaa aggagaactt tacttacatt ctcaacatcc atatacaaga
     6421 gcacttatga gcgcagtacc agtacaagac cctacaatta agaaaaatag aattatatta
     6481 aaaggagaca taccatctcc aacaaatcca ccaagtggat gtaagtttca tacaagatgc
     6541 ccttattgta aggaaatatg caaaaaagag attccagttt taaaagaaat atcaagaaat
     6601 cattttgttg catgccatct agtatagata tttcaaaaat taaatttgat gtatataata
     6661 agaataatag aataaactga aaattttttt gattaagatg ggatttcatg ttgttaatat
     6721 taagttagtt gatatataag tttaatttat aatattatta ttgaaaagag aggtagttat
     6781 gtcaacttta gtaacattgt tgggattatt ggtttgtaca aagatttcaa ctgtatttag
     6841 caaaaagtgg agtaatattc ctcttgcaat atatcaaatt gtactaggga ttatattgtc
     6901 tattttgccg tttaaattat cattttcatt taatccagaa atatttgtaa tatgtatcat
     6961 agctccatta ttattcagtg aaggacaaaa tgtatcaaga aaagagttac tagagcttag
     7021 aaaaccaatt ttgcttcttg catttggttt agttttgata accgtgtttg caggtggaat
     7081 ctttatacat tttcttattc ctaggatgcc attatctgtt tcacttgctt tggcagcagt
     7141 aatatcttca actgatttag tagcagtaaa atcaatcaca caaggattaa attttcctaa
     7201 aaatatgatg agtatacttg aaggtgaatc tttattaaat gatgatgata gaatcataaa
     7261 tataatggaa taaaatttga tttttgatgt taaaaataat actaaaaaat aggaaatagt
     7321 tttcaataat ttacttgaca tatatattta tttataatag aataaaacca taaattgaat
     7381 acaatatata agaattaaca agctggattg ttactggtaa gcaggcaata cctaggttaa
     7441 ggtatacagg tgaattgtat cttaatttag gtattttttt atgaataagg gaaattgtat
     7501 tttgggagag atattgatta tgattattca acaaatttat aataataatg ttgtattagt
     7561 tttagatgaa aatataaaaa aagaattaat actaacagga tgtgggattg ggtttcaaaa
     7621 aaagaaaggt caagaaatag ataaaagtaa aattgaaaga acttttgtaa tacaagatga
     7681 aagcttttta gataaaatta gcaaactagc aagccaggtt gatgagaaat tttttgagat
     7741 tagtactgaa ataattgctt atgcagagga gaatttgaat actaagcttt atgaatatat
     7801 ttatgtagca cttactgacc atattgcatt tgcaattaaa agatatcatg aaaatataac
     7861 tataaagaat gatttactac atgagataaa aagaatacat aaaaaagaat atgagattgg
     7921 taaatgggct gtagattata taaataaaga atttgatgtg aaatttccag tagatgaagc
     7981 tggttttata gcaatgcata tagtaaattc aaactataag ggaagttcaa aggaatcact
     8041 gttaattact aaaattgtaa aagacatact aaatattata agatattatt atagagttga
     8101 atttaaagaa gatgatataa attatgatag gcttttgact catttaaaat tctttgcaaa
     8161 acgattagta aaaaaagaaa aaatcaatga tacaaataat gaaattatag atataattaa
     8221 agtaaagtat gaaaaggatt atgattgtgc atataaaata aaaactcatg tagaaaagaa
     8281 ttatgattac tatgtaagtc aagatgaact actttattta acattacata tcaaaagagt
     8341 tatatctgta ctgaatttat aataaagagt agtttaatac agaatgtata ggtttaatat
     8401 attatgttta attttaatat attatttaaa atttaatata aaataataat tttaaaacat
     8461 taagattgtt actgataatg caggcaagac ttatgtttat aaaaaaatta taatagttta
     8521 tttttaattc tgaaattagg ttaatatagg tttatttata ttaacataag tttaatttta
     8581 gaattaagta tactaatgta actttttata aacttaagtc ttttttattt aaaaaaatat
     8641 aaaataataa ggagagaaaa gtaaatgaaa aaagtatttg gtgttttaca aaaagtagga
     8701 aaatctctaa tgctaccagt tgcattactt cctgcagcag gtatattact tggcgtgagt
     8761 aatgctttag caggagacgc attgttgtct cattttccag ctttggcgaa tcctacattt
     8821 caacttataa taagtataat ggaaagttca ggtcaaatta tatttaataa cttaccactt
     8881 atatttgcag ttggggtagc tgttggttta actgaaggag aaggtgttgc agcattggca
     8941 gccatagtag gttttttaat tctcaataca tctatgggtg ttatggcggg tgttacagaa
     9001 gatatggtag acaattctat gtatgcaagt attgttggaa taccaacaat tcaaacaggg
     9061 gtatttggtg gtattattat agggatagtg gctgcttatc tttataaaaa gttttataaa
     9121 attgaattac ctcagtatct aggttttttt gcaggtaaaa gatttgtacc aatcgttact
     9181 tcattagttg ctatagcaat aggaattata ttgtctttta tttggcctcc aatacaagag
     9241 ggattactta gtttctcaaa aaatgttata gaagcaaatc aaacactagc agcatttatg
     9301 gaaggttctt tggaaagact attaatacca tttggtttgc atcatgtttt ccataatcca
     9361 ttttggtatc aatttggaga atatgtaaat aaagctggtg agcttgtaat gggtgaccaa
     9421 aagatattct ttgctcaact taaagatgga gtaaatttca cagcaggtac ttttatgaca
     9481 ggtgcatttc catttatgat gtttggtctt ccagcagcag ctcttgctat ggtgcatgaa
     9541 gcaaagccag aaaagaaaaa atttgtagca ggtataatgg catcagcagc attaacctct
     9601 tttttaacag gaataaccga gccaatagag tttgcgtttt tatttgttgc accagtatta
     9661 tttgcaatac attgtttatt ttcaggttta tcatacgcaa ttatgcaatt attaggagtt
     9721 aaaattggat tcacattttc aggaggatta atagatttct tattatttgg tgttcttcct
     9781 aatagaactc catggtactt ggctatcttg gtaggtattg gttttgcagt attatactat
     9841 agtgtattta gatttatgat aagaaaattt aatttaaaga caccaggaag agaagatgag
     9901 tcagaaacta acgctgaagt cattgaaatg tctaagaatg aattagcatc agagatttta
     9961 attgctttag gaaataaaga caacttaaag agcctagatg cgtgtataac aagacttaga
    10021 ttggttgtaa atgatataaa taaagtagat aaagaaaaat tgaaattact tggagcagca
    10081 ggtgttatgg ttattggcaa caatgttcaa gctatatttg gaccaaaatc ggattcaata
    10141 aagtctgata taaaagatat aatagaggga aaaacaaagg ttgaaagtac tgttactaaa
    10201 aatgttgata aaaataaagt gaaaaaaaat atggaagttg ccaatatgga aaatgcttta
    10261 gtagaaaaga ttgattcatt aacaaatgga gagatattag atatagaaga agtgccagat
    10321 tctgtatttt caagtaaact gatgggggat ggatttgcaa ttaaatctag tgatggattg
    10381 atttattcac cagttgatgg aactattgga gttatatttc caactaagca tgcaataata
    10441 ataaaatcca aaaaaagtgg agtagaaata cttatacatt tagggataga aactgtaaat
    10501 ttagaaggaa atggatttga agtatttgtg aatgtaggag atgaagtaaa agcaggagaa
    10561 aaattagtta aaatggactt agaatatatt gaaaaaaatt gtctatcaac aataagtcct
    10621 gtagtattta cgaatttaga acaaaatcag aaattaagca ttaaaaaagg aataactaca
    10681 gcaaaagagg agaacagagt aagtatatta agatatgata ttgaaaaaat aggatagcta
    10741 tatgatagta taactaaaag ttatgtgaga tttaataaat atataaatag gttcagttaa
    10801 catgcaattt atttaatata tattttaagt aatagaaata tatatcatta tattttgata
    10861 tattttaaa
//
LOCUS       DACTGW010000132        10727 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.132, whole genome shotgun sequence.
ACCESSION   DACTGW010000132 DACTGW010000000
VERSION     DACTGW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.132"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            223..1419
                     /locus_tag="I9573_002503"
     CDS             223..1419
                     /locus_tag="I9573_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAT4797113.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1512..2036
                     /locus_tag="I9573_002504"
     CDS             1512..2036
                     /locus_tag="I9573_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAT4797114.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            2061..2237
                     /gene="rpmF"
                     /locus_tag="I9573_002505"
     CDS             2061..2237
                     /gene="rpmF"
                     /locus_tag="I9573_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAT4797115.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2444..3001
                     /gene="fapR"
                     /locus_tag="I9573_002506"
     CDS             2444..3001
                     /gene="fapR"
                     /locus_tag="I9573_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HAT4797116.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            3012..4034
                     /gene="plsX"
                     /locus_tag="I9573_002507"
     CDS             3012..4034
                     /gene="plsX"
                     /locus_tag="I9573_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAT4797117.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            4060..5046
                     /locus_tag="I9573_002508"
     CDS             4060..5046
                     /locus_tag="I9573_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAT4797118.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5155..6084
                     /gene="fabK"
                     /locus_tag="I9573_002509"
     CDS             5155..6084
                     /gene="fabK"
                     /locus_tag="I9573_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HAT4797119.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            6100..7050
                     /gene="fabD"
                     /locus_tag="I9573_002510"
     CDS             6100..7050
                     /gene="fabD"
                     /locus_tag="I9573_002510"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAT4797120.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIEILLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            7050..7799
                     /gene="fabG"
                     /locus_tag="I9573_002511"
     CDS             7050..7799
                     /gene="fabG"
                     /locus_tag="I9573_002511"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAT4797121.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTRVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7853..8077
                     /gene="acpP"
                     /locus_tag="I9573_002512"
     CDS             7853..8077
                     /gene="acpP"
                     /locus_tag="I9573_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAT4797122.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            8106..9344
                     /gene="fabF"
                     /locus_tag="I9573_002513"
     CDS             8106..9344
                     /gene="fabF"
                     /locus_tag="I9573_002513"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAT4797123.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9570..>10727
                     /locus_tag="I9573_002514"
     CDS             9570..>10727
                     /locus_tag="I9573_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAT4797124.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLNIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMKSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEK"
BASE COUNT         4293 a         1138 c         2163 g         3133 t
ORIGIN      
        1 ttagtgaaat acacaattta ccatactaga aatatgatat aatataaaaa aaaagtatat
       61 acttttatat taaaaggtga tatattattt atgaaaatcg tttaatgtat acaataatat
      121 atctataaca aaataataaa agatagagtt ttatttagac atttattaca aatcaaaatt
      181 tattacatat tatttataaa aaaatctaag gaggaaataa taatgaaaat attagtatta
      241 aactgtggga gttcttcatt aaaatatcaa ttaatagata tgaataatga agaagtttta
      301 tgtataggat tggttgaaag aataggaata gaaggttcta tattaaaaca tgaaaaagca
      361 ggtagagatg ataaatacgt tgttgaacaa ccaatgaagg accacaaaga tgcaatagca
      421 ttagttttag aagctgttgc tcatccagaa tttggtgcag ttaaagaaat gaaagaaata
      481 gatgcagtag gacatagagt tgtacatgct ggagaaaaat ttgcaacttc agtagtaata
      541 actccagaag tagaagaagc tttaaaagaa tgtatagatt tagctccact tcataatcca
      601 gcaaacataa tgggaataga tgcttgtaaa gctatacttc cagatgtacc aatggtaggt
      661 gtatttgata ctgctttcca tcaaacaatg cctaaatctt catacttata tggtttacct
      721 catgagctat atactaaata tggggtaaga agatatggat tccacggaac ttctcataat
      781 tatgtatctc agagagcagc agagatatta ggaaaagata taaaagattt aaaaatagta
      841 acttgtcact taggaaatgg tgcttctata gctgctgttg atggtggaaa atgtgtagat
      901 acatctatgg gattcactcc attagaagga ttaataatgg gaactagatg tggagatata
      961 gacccagcta tattaccatt cttaatgaga aaagaaggtt tagatgctga tggattggat
     1021 aaattaatga ataaagaatc tggagtatat ggaatgactg gaatttctag tgacttcaga
     1081 gatatagaag atgcagctaa aaatggagac gaaagagctc aggctacatt agaagcgtat
     1141 gttaaaaaag tacaaaaata tataggcgct tatgctgctg aaatgaatgg attggatgtt
     1201 gtagtattta ctgcaggcgt tggtgaaaat ggaaaggcta taagagcaga tattgcttct
     1261 aacatggagt ttttaggaat gaaattagat aaagaagcta atgatgttag aggaaaagaa
     1321 acagtaatat ctactgctga ttctaaagta aaaatgcttt taataccaac taatgaagaa
     1381 ttaatgatag ctagagatac attaagattg gtaaaataat taaattaaac ctatgcctat
     1441 caagtaaaaa tacttgacaa acaaatgcta gatttgtata ataaatttgg tgatatgttg
     1501 aggtgaactt aatgaaagtt agcatagaaa aaataaatag aaaagaaact gacaaaatag
     1561 acttgaattt ttgtgaaaaa attgatacta ttagttattg tgatgagatt tacaaattag
     1621 tatcacctgt aaatttaaaa gggaaagtgt caaagactaa taaaggtttg tatctagata
     1681 tagatgtcaa ttttacaatt gttgacaatt gttcaagatg tcttaaagaa gtagaaatac
     1741 cactagagta ttctatacaa ggttttttag taaaagaaga agattatgat gaagatgagt
     1801 ttgaggaatt tgatcctttt atattcgatg gtgaagaaat cgaccttatt gatataatag
     1861 aacagacatt agattttaat gtacctcata aagttctttg tagtgaaaac tgtaaaggtc
     1921 tttgtcaagt atgcggggca aacttaaatg aagaagagtg ttcttgcagt gaaattacaa
     1981 acgatgagga atacatagat cctcgttttg ctaaattaaa agatttattt aattaaattt
     2041 tgactaagga ggtggcgtaa atggcagtac caaagcgtaa aacgtctaaa tcaaacacaa
     2101 aaatgagaag agcagctaac tcaaagatgg aagcaacagg atttgtaagt tgcccacaat
     2161 gtcatgagcc aaaattacca catagagtgt gtccagactg tggatattat aaaggtaaag
     2221 aagttgtgtc taagtaatag cttctaaaaa gcatgtagag tatactctac atgttttttt
     2281 tgtacatata aaaaaataag atgatttact agagaaaata agtggatttt atgcatatta
     2341 aaaaatagaa aaataataat ataagtattg aaaatttttg tgtgttacta tatactacta
     2401 ttagtagctg gtactaatta cagctataaa atttggaggg aacatgaaaa agaaaagtaa
     2461 agcccaaaga caaaaagagc ttatagatat gctaaagaca gatccttttt atactgatga
     2521 agaattatca agtctgtttg atgtaagtat acagaccata agattagata gaatgagtct
     2581 aaacatacca gagctaagag aaagagtcaa atcaatagca gaaactcaga gttcaaaagt
     2641 aaagacttta ggagtaaagg aaattacagg agaaatcatt gatttatctg tagggcgatt
     2701 aggtatatct atgttagaag ttacacagga catgatttac tctaagacta atactttaaa
     2761 agatacgtat attttttctc tagcagattc attggcaatg gctataatag atgcacctaa
     2821 ggttattatg agagttgcaa atgtaaaaag ttttaagttg atagaacaac aagatagact
     2881 tatagcaaaa gcagaagtgt atagaaatat agataaaaag cattatgtaa aagttgttat
     2941 caacaataaa gcccaagagc aaatatttag aggaaagttt atctttgaag aattagatta
     3001 ggagggaaat tatgaaaata gtaattgatg gaatgggtgg agataatgca cctaaatcta
     3061 atgtagaagg cgcagtaaat gctataaaag aatatcaggt tgatttaata atcacaggag
     3121 ataaagattt acttgaaaag gaattttcaa attatgaatt tgatagaaat aaattagaga
     3181 tagttcatac tactgagatt atagaaaacg aagataaacc agtaaaggct ataagaagta
     3241 aaaaagattc atctatggta gtagcattaa atcttgtaaa agaaggaaag gcagatgcta
     3301 taatatcagc tggaaataca ggagccttac ttgctggtgg attatttgtt gttggtagaa
     3361 taaaaggaat cgatagacct tgcttatgtt cagcaatacc aaatgtaaag cgaggaatga
     3421 cacttatagc agactgtggc gcaaatgcag attgtaaacc taagaatcta gttgaatttg
     3481 cagctatgag taatatttat tcaagaaaag ttttaggtct tgaaaatcct aaggtagcat
     3541 tagcaaatgt tggactagaa gaaggcaaag gcaatgattt agtcaaaagg tcatatgaag
     3601 aaattaaaaa attagattta aattttatag ggaatgtaga agctagagaa gttataaatg
     3661 catacacaga tataatcata tgtgatggat ttacaggtaa tatattactt aaatctgctg
     3721 aaggtgtagc actttcagtt atgagtctta taaaggaaac ttttatggcc agtacaaaaa
     3781 gtaaaatagg agcattactt ataaaggatg atttaagaaa attaaaaagt tttatagatt
     3841 attctgagta tgggggagct ccacttttag gtctaaatgg aggagttata aaagctcatg
     3901 gtagttctga tgctaaagct ataaaaaatg ctataaatca aggaataaaa ttttcaaagg
     3961 gaaaagtcgt tgaagatatt aaccaattta taagcaaata caatgaagaa aataaaaata
     4021 acgaagatga ataaactcgt tttggaggtt taatatataa tgaatacaaa ggctggaata
     4081 ttaggagtag gaagttatct acctgagcaa tcatatgata attttcactt cgaaaaaata
     4141 atggatacat cagatgagtg gataagtact agaactggta ttaaggaaag aagatttgcc
     4201 aaagagtctg aagcaacttc tgacttagca tctaaagctg ccttaaaagc tatagaatgt
     4261 gcaaagttaa acgtagagga tatagagctg attatattag ctacaattac tcctgatatg
     4321 tctttgcctt caactgcatg tatagtacaa gatgcaatag gagcagtgaa tgctactgca
     4381 tttgatatat cagcagcttg ttctggattt gtatatggag taactattgc aaagcaattt
     4441 gtggaaacag gttgctataa aaatgtgctt gtaataggag cagaaacttg ttctaagttt
     4501 ttaaattatg atgatagaac aacagcagta ttgtttggag atggagctgg agcagctgtt
     4561 attggtcctg taaatgaggg tggaatactg tctacacata tgggctctga tggtaaagga
     4621 aaagattgtt taaaagtacc tgctggaggt tcaagactta aggcaagtaa agagactgta
     4681 gaagctaatc tacatacaat agaaatggca ggtagtgatg tgtttaaatt tgcagttaga
     4741 aagatggcag aaacttcttt aagagctctt gaaaaagcaa acctaaatac aactgatata
     4801 gactacttag tgccacatca agctaatata agaatcatac aagcttcttc taagagatta
     4861 gagcttgata tgaaaaaagt ttatgtaaat atagacaaat atggaaatat gtctgctgca
     4921 tctataccag tagcattaga tgaagcttat agagaaggta aaattaaaaa gggtgataat
     4981 gtagttttag ttggctttgg tggaggtcta acttgggggg cttcagttgt caagtggact
     5041 ttatagtata aaatatctaa aatgatttaa ttaacaaaat taaacttata cacaaaataa
     5101 agttgattaa taataaaaag tttactttta tgatttcaag tgaaggagaa ttgtatgaat
     5161 aaaatttgca aaatattaaa tataaaatac ccagttatcc aagggggaat ggcatgggta
     5221 gctactgcat cattagcaag tgctgtatct aatgcaggag gacttggcat aatagcagca
     5281 ggaaacgcac caaaagaagc tataaagaaa gaaattgttg agtgtaaaaa attaacagat
     5341 aaaccttttg gagtaaatgt aatgcttatg tcgccatttg ttgatgatat aattgatttg
     5401 attatagaag aaaaagttca agttattact actggtgctg gaaatcctgc aaaatatatg
     5461 gatagattaa aggaagctgg aacaaaggtt attcctgtag tacctacaat agctttggca
     5521 caaagaatgg aaaagttagg agctacagca gtaatagcag aaggtactga aggtggagga
     5581 catataggag aacttactac tatggtctta gttccacaag ttgctgatgc tgtaaacata
     5641 cctgtaatag ctgctggagg aattgtagat ggtagaggaa ttgcagcatc atttgcatta
     5701 ggtgccagtg cagttcaagt aggaactaga tttatttgca gtgaagagtg ttctgtgcat
     5761 tcaaactata aaaacttagt actaaaagca aaagatagag atgcaattgt aacaggaaga
     5821 agtactggtc atccagtaag aacattaaaa aataaactat caaaagaatt tttaaagatg
     5881 gaacaaaatg gagctactcc tgaagaattg gataaaaaag gtacaggagc tttaagattt
     5941 gcaacagtag atggagacat agaaaaaggt tcatttatgg caggtcaaag tgctgctatg
     6001 gtaaaagaaa taacaccttg taaggaaatt atagaggcta tggtaaatca agcaagagag
     6061 attatgccag caatagaact gtaaatcagg aggagcaaga tgggaaaagt tgcactagta
     6121 ttccctggtc aaggagctca atatgtaggt atggccaagg atttatatga aaataatgct
     6181 gttgctaaaa gtgtaataga tgaggctagt gatgccttaa atatggattt aaaaaacctt
     6241 atgtttaatg gaaatgaaga agaactttct aaaactgaaa acactcaacc tgctatggtt
     6301 acacatagtg tagcagtatt aaaagcggta caagctcaaa tagatttaaa atataatgca
     6361 tgcttaggat tatccttagg agagtatagt gcgttggttg cagcagatgc aattgacttt
     6421 aaagatgctg tatgtttagt taaaaaaaga ggtaagttta tgcaggaaac agttccagca
     6481 ggtgttggag ctatggcagc aattttaggt ttagatagaa atgtacttga aaatgtggtt
     6541 aaagatatac aaggaggtat tgtagaagta gcaaattaca attctcctgg acaaatagtt
     6601 atatctggtg aaaatgaaaa gatagaagaa gcaatggtaa aatgcaaaga agcaggagca
     6661 aaaagggcag ttaaattaaa tgtaagtgga ccttttcact catcaatgtt aaaagaagct
     6721 ggtgtaaaat tggccagtga attagaaaaa gttaacataa ctaaacctaa agttgatgtg
     6781 gtggcaaatg taaatgcaga ttactataaa aacgaaggta aagacctgct tataaaacaa
     6841 gtaagttcat ctgttctttg ggaagattct atagaaatac ttttaaatga tggatatgat
     6901 acttttatag aaatgggacc tggaaaaact ttaaaagcat ttattaagaa gatagcttcc
     6961 aataaaaaag caaatgtcaa tatttataat atagatggta tagattctct taatgaattt
     7021 gtacaaaatt atagaaatgg agagatataa tgataaatct tacaggacaa gtagcagttg
     7081 ttactggtgg ctctaggggt ataggaaaag aaatagcaaa aaaactagca tcttttggag
     7141 ctgatgtagt aatcaattat acttctaaag aagatgaagc actaaaaact aaaaatgaaa
     7201 tagaaagtat gggggtaaag tgtacctcta taaaatgtga tgtgtctaaa tttgatgaag
     7261 tgaatcaaat gatagattct gttgtaagcg aatttggaaa aattgatata ttggttaata
     7321 atgcaggcat aactaaagat ggtctgctta tgagaatgaa agaagaagat tttgatagag
     7381 ttatagatat aaacttaaaa ggtgtcttta attgtacaaa agcagttact aaacctatga
     7441 tgaaaaagaa gtatggaaga ataataaata tgacttcagt agttggaatt atgggtaatg
     7501 cagggcaaac taattattgt gcatcaaaag caggtgtaat tggatttaca aaagcttctg
     7561 caagagagtt agcatcaaga aacataaata taaatgcagt agcacctgga tttatagaaa
     7621 cagatatgac tagagtacta agtgatgatg taaaagaatc aacactagca aacataccaa
     7681 agaaatctta tggtaaacca gaagatgtag ccaatgccgt agcattttta gttagtgaca
     7741 tgtcaagtta tataacagga caagtaataa atgtagatgg tggaatggta atgcaataat
     7801 aatttaaaaa gtagagaaat ctattaaaat aaaaaatttt aggaggtaca caatgtttaa
     7861 taaagttgta gaaataataa tagagcaatt aggagtagaa gataaagaaa taactatgga
     7921 gacatcatta atgaaggatt tagaagcaga ttcattagat gcagtagaaa taataatggc
     7981 acttgaagat gaatttggta tagaaatacc agatacagaa gcagaaaact tcaaatctat
     8041 aggagatata gttaagtata tagaagctaa taaatagtcg agctttaaaa aagtggaggt
     8101 atccgatgaa taaaagagta gtaataacag gacttggatg tgttacgccg ttgggaacgg
     8161 gaaaagaaga gttctggagt aatataaaat ctggagtatc tggtatagat aaaataacta
     8221 attttgatgc tagcacatat caaactcaga tagcaggaga agttaagaat tttcatccag
     8281 aagaatatat aagtaaaaaa gaattgaaga gattagataa atttgctcaa tttgcaatag
     8341 tatctgctaa attagcagtt gaagatgcaa atcttgattt agataaagtt gatagagaac
     8401 gttttggtgt tataataggt tctggaatag gtggagttga agctatagaa actcaacata
     8461 aaattctttt ggaaaaagga aataaaagag taagttcact ttttgtacct atgatgatag
     8521 gaaatatggc agctggtcaa gtttcaatat ttttaggagc aaaagggcct aacactaatg
     8581 tatgtacagc ttgtgcatca ggaactcatt caataggtga tgcttttaag gtaattcaaa
     8641 gaggtgatgc agacatcatg gtagcagggg gctctgaagc tgctgtaaca ggtcttgctt
     8701 ttgctggatt ctgtaacatg aaggctatgt caactagaaa tgatgaccct aaaactgcat
     8761 caagaccgtt tgataaagac agagatggtt ttgtaatggg agaaggagca ggtattgtta
     8821 tcttagaaga cttagaacat gctttagcaa gaggggctaa aatatatgca gaagtagttg
     8881 gatatggttt aactgctgat gcataccata tgacaactcc agctgaaaat ggagagggag
     8941 cagctaggtc tatgaatatg gctttgaaag atggaaatgt tccattagaa gaagtagatt
     9001 atataaatgc tcatggaact tcaacttatt acaatgattt atatgaaact atggctataa
     9061 aaactgtatt tggagaaaag gcatatgact tatgtgtgtc atcaacaaaa tctatgactg
     9121 gtcacttatt aggagcatca ggtgctatag aggctgttgt ttgtgctatg agtatagaag
     9181 atagttttgt gcctccaact ataaacatac aagaagttgg tgaagatttg gatttagatt
     9241 atgttccaaa tcaaggtaaa gaaaaaaata ttagatatgc actatcaaat tctttaggat
     9301 ttggtgggca taatgctaca atagtactaa aaaaatatgt ataaataaat attaaatata
     9361 aaactaaaaa acaagcactt gttttaactg tataaattta tattagctta aatttaaagt
     9421 ctcctaaatt attttaggag acttttttat taagatttaa gtctagcata tgttgtaaat
     9481 cattttgata tattgtatta ttaattatta agaggttatt tatatatgca agttttcatt
     9541 ctacaatata caaaaggagg actaaaattt tgaattttag actaccaaag aaaaggttac
     9601 ccaaaaagat aatttttata tgtataatac ttattagtat tattttgttt ataaagtatg
     9661 accaaaatgg attgaaagaa agagaaatta taaataaggg aaaatatatg tctgaaagcc
     9721 ttgtggcaga taaggagtat gatgaagcaa aaaaagtggt taagacttct ttcgaatcta
     9781 ttccagaaaa aaaatacaat aaagtatata atgaagtttg ggatatgtta aagactattt
     9841 catatgttcc agatggaacg aagatagtca ttgattcttt agaaaagatg agagaatcag
     9901 atgtgaaatt atctgatgaa tgtagattta atatagaaaa aagattagca tcagtgtata
     9961 taatggataa taattattct aaggcagtag atttaactgt aaagtctata aaattggctg
    10021 agaaattggg gaataattat gaaaaagcaa gactagatgt tgatttggcg agcatatttt
    10081 taaatatagg tagctatgaa acaggagaga aattaataaa ggattctttt aaaatagata
    10141 ttgatgaagg tgaaaaaaat ggaatggtta gattatatgc tattataaat ctagcagaaa
    10201 tatatgtaga agagggagaa tatgataaag ctattgaaat aagtagcagg ataaaagatt
    10261 ataaaaagtt tgtaaatacg gataattaca atgattttga tataatggcc tctataatga
    10321 agtcaaatgc gtatgtgggc aaaaataata tacaaaaggc aaagactttt ttagaaaaag
    10381 ctgattatct tataaaatca gatagaacag tttatatatt agataaagat atgtactatt
    10441 atatggcaat gggaaactta gaatataaaa gcgaaaatta taattctgca atagataatt
    10501 ataaaaaaag tttagagata tctaccaaaa gaaagttaac tcaggagaaa ataaaaaatt
    10561 taaataagat attaaatata tatgaaaaac aaggaaatat agaatcttta aatgagtatc
    10621 aaaagatgtt aataaatgaa tataaagaga acaaaagtat aagagattca gagactagtt
    10681 tctatataat tgataaagta tcaaacgaaa gtaagctatt tgaaaaa
//
LOCUS       DACTGW010000133        10718 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.133, whole genome shotgun sequence.
ACCESSION   DACTGW010000133 DACTGW010000000
VERSION     DACTGW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.133"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            265..1449
                     /locus_tag="I9573_002515"
     CDS             265..1449
                     /locus_tag="I9573_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797125.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(1826..2935)
                     /locus_tag="I9573_002516"
     CDS             complement(1826..2935)
                     /locus_tag="I9573_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAT4797126.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            3398..4591
                     /gene="nifV"
                     /locus_tag="I9573_002517"
     CDS             3398..4591
                     /gene="nifV"
                     /locus_tag="I9573_002517"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HAT4797127.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVI
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            4616..6541
                     /locus_tag="I9573_002518"
     CDS             4616..6541
                     /locus_tag="I9573_002518"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HAT4797128.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSNVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            6563..7558
                     /locus_tag="I9573_002519"
     CDS             6563..7558
                     /locus_tag="I9573_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HAT4797129.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGAEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            7885..8358
                     /locus_tag="I9573_002520"
     CDS             7885..8358
                     /locus_tag="I9573_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT4797130.1"
                     /translation="MVGSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            8392..8886
                     /locus_tag="I9573_002521"
     CDS             8392..8886
                     /locus_tag="I9573_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4797131.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            8983..9504
                     /locus_tag="I9573_002522"
     CDS             8983..9504
                     /locus_tag="I9573_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAT4797132.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMYDDNIEADKYIREELKVPSKYSVECIISIGYSDEEKKAYND
                     SDLDYKKVHFNNF"
     gene            9693..10004
                     /locus_tag="I9573_002523"
     CDS             9693..10004
                     /locus_tag="I9573_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAT4797133.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            10032..10718
                     /locus_tag="I9573_002524"
     CDS             10032..10718
                     /locus_tag="I9573_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAT4797134.1"
                     /translation="MKYSYKKCIIDSILLMFIIQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         4114 a         1170 c         1938 g         3496 t
ORIGIN      
        1 aaaaattaaa taataataaa gaaaaaatgt ataatattta acattaaaaa agatatttca
       61 taaaattaaa aaacaaagtg aatttgaata ctatttaaaa aataaaaaaa tatataaaaa
      121 atatttaata caaaggtaga aataagttga aaatgtaaag tcattggaaa aatatagttg
      181 aatttaatga acaaaaatgt tattgtttta tcaatgaagt gttaaaataa aaactttcaa
      241 tatcatttta gggaggatga tttcatgcaa aaaagtgtaa agaaagcaaa agtaacggga
      301 tctatgttag ccatttttgg tgttgcaagc gttcttttta gttcacatgc aggaggagga
      361 tttgctacag ggaatcaaga aactcagtac tatgtgcagt atggatggac agcaccactt
      421 atggcaattt tagcaatgat tatacttact gctaccatga gagaagttat cataatgtat
      481 aataataaca attgtaggaa ttataaggat ttattttgtg agctttggag accatatcca
      541 aaacttgaaa taatatggga gatatattac tatctaatgg ttttaatagc tgtaagtgct
      601 gttatagcag gtgcagcagc agtatttcaa agtataggag taaattattt tgtagctgta
      661 tttattattg gagttgtact tcttgtcttt actatatttg gtgctatgct tgtatctaaa
      721 gcagctacag caatgacaat agctattcta gtatgtactc ttactatttt tattgttggt
      781 ataaaagcta aagtaccaga aataactgaa atattatcaa atagaacgag tttcacacct
      841 ggctatatca agccaatact taatactttt atttatgcag gttttcaatc ggttgttata
      901 cctacacttg caggatgttc aagaccactt cttaagaact caaaagaagc tactaaggca
      961 atgataattt catttgttat gaatgcaatt gcattaggac ttgctgtaac tatgctaatg
     1021 ggatggtata gagaaattat tgctgctggg caaacaacac tccctactct ttatgttgca
     1081 ggtcaatctg gaaatcatac tatatacata atttataatg ttgcattatt cctttgttta
     1141 atgtctacag gagtaactac tatatttggt cttgtaaata gatttgaaga ccataaagca
     1201 ctttctttcc tttcttcaag aatgaaaaga agggtattta cagcttgtgc aataatggta
     1261 gtgtcaatgc ttatatcact tacaggatta agtaatattg ttaaatatgg atatggatat
     1321 tgtggttatc ttggtctatt cactatagta attccattcc ttacactagg acattataag
     1381 aataagaagt ttgcaaagga aaatccagaa gctaaatggc cagcggaatt aaatgaaaat
     1441 gtaaattaga tgtagtagca aaaaatattg ctaaagagaa tactataaat aagaatacta
     1501 tagataatat atctgcatgt ttattaagat tatttgcaaa atctaatatt gaacaactat
     1561 aaacaagcaa tataaaacta agaataaaag ataaaaagag ggtgtctcaa aatggacttt
     1621 tagtttataa ggcactcttt tttatatgaa gtcaaatata ttttcattat tttgacaata
     1681 aaagagacta atcctacttt ggatagcctc ttttttcttt aatataaaat agtataacag
     1741 caaagtatag attgatacat gttttctgta gctttctata tattacagtg tttgtgtggc
     1801 attggtattt taagcaatct ataaattata attctataat tcttctacta attttagatt
     1861 ctatagccgt ctctatttca cttaacattc gttcattttt agggtcaata aaagcacatg
     1921 tatagccttc ttcaccagct ctaccagttc taccaatacg gtggatgtag tcttctggtg
     1981 tttctggtaa atcgtaatta tatatatgac ttactccaga tatatcaagg ccacgagaag
     2041 ctacatctgt agctattaag tattgtatat ctaaatttct aaatgatttc attattcttt
     2101 ctcgttttga ttgagcaata tcactatgaa gtttttgaca attatatcct cttatagcaa
     2161 gagcttcttc aagattgtca acccttctct tagtcctaca aaatattatt gccataaaag
     2221 gattatcttc atctagtacc ttacataagg catctaattt ttttctatct gtagtttcta
     2281 ctacttcttg ttttatatta ttaagagtga tttcttcttt ttgaatagaa actacaacag
     2341 gattttttgt gtatctataa gctaattttt ttacttgaga atttatagtt gcagaaaagc
     2401 atagtgtttg ctttttgttt gaagtctctt ttaaaatagc ttccacttcg tttttaaatc
     2461 ccataagcaa catttggtct gcttcatcta aaacaaaagt ctttaactta ctaaggtcta
     2521 ttgtttttct atttagatgg tctaaaagcc tacctggtgt agctataatc atatgaatat
     2581 ttccttttag tttctttatt tgagaaccaa tatcttttcc tccatatgct gcaagaatac
     2641 ttacatcttt agattcttta agtttcatag cttcttctgt tatttgaata gctagttctc
     2701 ttgtaggaga aagtataagt acttgtatat catttatatc taatgatata ttttcaaata
     2761 tcggaagtaa aaaagctagt gtttttcctg ttccagtttg agcttctgct attacatctt
     2821 ttccttcttt tattagttta atactttctt tttgtatagg tgtaggtgtt gttattcctg
     2881 atttttttag aatatttatt gtattttcac taatccctaa gcttttgaaa tccattgtta
     2941 tcttaccttt catgaaaaat tttctttttt attaaccaat taataatatc atatttgtaa
     3001 gtatttttag taaatttttt aattattatt tatatagctt aaaaatatat taagatactt
     3061 taaaatggaa tgtaacttaa cttaaatttt agtaggaatt ttataggttt atttttaaag
     3121 gttttttgaa aataaatcta tttaaaaatt aaattaaaat gcaagaaaaa cataataact
     3181 ttcattttta agaagttttc acaaaaaaga aaaaatatag tgcaataaat gatacaaagt
     3241 aatataatag attacaagat atgaaaagct aaaaaaataa attgcaaatg aaattaaaat
     3301 gaataaaaaa taaaaaaata atcataagtt agaggtatca atgaaaaata agacttaatg
     3361 tttttaaaat taaaagtata ctaagggggg cattaatatg tgcgttatat caaaagatag
     3421 agcaaaggaa ataaaaatag tagatactac tttaagagat ggagagcaaa cagctggggt
     3481 tgtatttgca aatagagaaa aaataatgat tgctgaaatg ttaagtgacc taggtgttga
     3541 ccaaattgaa gttggtatac ctactatggg tggagatgaa aaaaatgtaa taaagcacat
     3601 atgtagtaga aatttaaaat cagatataat ggcttggaat agagcagtaa taaaagatgt
     3661 tgaagaatct atttcttgtg gtgttgatgc agtagctata tcaatatcag tatctgacat
     3721 tcatatagaa aataaattga gaacttctag aggctgggtt ttagaaaata tggctaaaac
     3781 agttgaattt gcaaagaaaa atggattata tgtttcagta aatggagaag atgcttcaag
     3841 agcagatata gacttcttaa ctgaatttat aaatgttgga aaacaagcag gggcagatag
     3901 atttagatac tgtgatacag taggagttat gaatcctttt tctataaaga atgcaataga
     3961 aactctttat gaaagaacta attttgatat agaaatgcat acacataatg actttgggat
     4021 ggcaactgca aatgcacttg caggaatagc agcaggagca aattatgttg gtgttacagt
     4081 aaatggatta ggtgaaagag caggaaatgc agcattagaa gaagttttaa tggcacttaa
     4141 gtgtgtttat aaatgtgatt taaataatat agatactaga aaatttagag gaatatgtga
     4201 gtatgtagca caggcatcag gaagaatact tcctacatgg aaaccagtag ttggggataa
     4261 tatgtttata catgaatctg gtatacatgc agatggagct ttaaaagacc cgcataacta
     4321 tgaaccattt gacccaagtg aagtaaatct tgaaagaaaa atagtaatag gtaaacattc
     4381 aggaagagct gcagttataa ataagttgag tgaatatgaa atgtatattt ctccagaaaa
     4441 cgctaccaag ctattaaatg ctataagagc tacatctata agattaaaaa gaagtttaat
     4501 ggataaagaa attcttcaat tatattgtga tatactagca catgagaaag gcacaacaga
     4561 agaagaagca gttagaggtt catatattta agatataatt gtttaggagg aaaatatggg
     4621 agataatata gtttataaaa taattaaaaa acatatagta gatggagaag ctgttgctgg
     4681 aagttcaatt ggtataaaaa tagaccaaac tttaacacaa gattctactg gaacaatgac
     4741 atatcttcaa ctagaagcta tgggaataga taaagtaaag actaaaagaa gtgtggcatt
     4801 tgtagaccat aatatgttac aacaaggatt tgaaaatgca gacgaccata agtatataca
     4861 aacagtggca gataaatatg gagtttattt ctctaagcca ggaaatggta tttgtcatca
     4921 agtattttta gaaagatttt caacaccagg agatacactt ttaggttctg atagccacac
     4981 acctactgct ggaggtgtgg gcatgatggc tattggagct ggtggattag atgttgcttt
     5041 agccatggca ggaggagcat actatataaa agcaccaaaa gtctgtaagg tgaatctagt
     5101 tggaaaatta aataatatgg tatcatcaaa agatataata ttagaagtgt taagaaaaca
     5161 aactgtaaaa ggtggagtag gtaaagttta tgaatatggt ggagaaggtg tcaaaagcct
     5221 atctgttcca caaagagcta caataacaaa tatgggggct gaacttggag caactacttc
     5281 aatcttccca agtgatgaaa aaacactaga attttttaag agtcaaggaa gagaagacgc
     5341 atggatagag ttaaaaccag atgcagatgc agtttatgat gaagagatta ctataaactt
     5401 ggatgaatta aaaccattag ctgcaaaacc tcatagccca gacaatgtag atgaagttga
     5461 aaatataggc aagattaaaa tagaccaagt ggcaatagga agttgtacaa attcttctta
     5521 tgaagactta atgaaagtag cacaaatcct gaaaggaaat aaagtacata aggatgtgag
     5581 tttggtaata gcaccaggtt caagacaagt tatggagatg attgctagaa atggggcttt
     5641 ggcagatata ataagtgctg gagctagaat actagaaaat tcttgtggtc catgtattgg
     5701 tatgggtcaa tctccaggta ctgactctgt gtctttaaga actttcaata gaaatttcta
     5761 tggtagaagt gggactttgt cagcacaagt atatttagta agtccagaag tggcagcagt
     5821 aagtgcaatc aagggagtgc ttacagaccc aagagaattt gatattaaat ttacaaattt
     5881 agatgtaaat gaatttttga tagatgatag catgataatt aaacctgctg atgtaggaag
     5941 taatgtagaa gtagttagag gacctaatat aaaacctttc cctcttaata cagagctaag
     6001 tcagtctata ggtggaaaag ttatattaaa gacagaagat aatataacta ctgaccatat
     6061 aatgccatca aatgctaagt tacttccttt tagaagtaat ataccgtatc ttgcaaacta
     6121 ttgtttcaat actgtagata cagaatttcc tcagagagca aaagacaata atggtggatt
     6181 tatagttggt ggagataatt atggtcaagg ttcaagtaga gaacatgcag ctttagcacc
     6241 attatatcta ggtgttaaag gggtaatagt taagagcttt gcaagaatcc acaaggctaa
     6301 cttgataaat agtggtataa ttccaatgga gttttgtgat gagaaagatt atgaaaatat
     6361 atctctttta gataatctgg agataccaaa tattttagac aatctaggta gtggaatttt
     6421 agaagttaag aatacaacta aaggaacttc ttttaaggtc aaagtagaat tgtcagcaaa
     6481 agaagttgac gtattaaaag ctggtggaaa attaaactac actaaaaatc aagctaatta
     6541 atatgaaaag agggaatatt gaatgtataa agtgacactt ataccaggag atggaatagg
     6601 accagaagtt gcgaaagcta tgaaaaaggt agtagaagct actggagcag aaatagaatg
     6661 ggaagaagtt aatgcaggag aggcagttat tgaagagtat ggtactccac ttcctgaata
     6721 tattatagat agtataaaaa agaacaaaat agctataaaa ggcccaatta ctactcctgt
     6781 aggtaaagga tttaggagtg taaatgtaac tttaagacag gcattagatt tgtatgtcaa
     6841 tttaagacct attaaaagtt ttaaggggat aaaatcaaga tatgaagatg ttgatttagt
     6901 ggttgtaaga gaaaatacag aagatttata tgctggaata gaacataaaa taggtgatta
     6961 tgcagctgaa agtataaaga taataacaag aagtgcaagt gaaagaatcg ttgattttgc
     7021 atgtaactat gttaaagata ataagagaaa aaaagttaca gctatacata aggcaaatat
     7081 aatgaaaatg tctgatggat tgtttttaga tgtgtttaga gaagttgcat caaaacatgg
     7141 tgtagaatat gatgatttga ttgtagatgc agcagctatg aacttagttt taaatccaga
     7201 aaattatgat gtaatggtaa tgccaaatct ttatggagat atactatcag atttaggtgc
     7261 tgggcttgtt ggaggtttag gtataattcc aagcgcaaat attggtaaag attgtgcgat
     7321 atttgaagct gttcatggaa gtgctccaca aatagcaggt caaaataagg ctaatccaac
     7381 agcacttatt caatcatcag taatgatgct tagatactta ggtgaatatg aaaatgcaca
     7441 aaaaatagaa acagctttag aaaaagtatt tcttgaagga agtaaattga ctgtagattt
     7501 aggtggctca gcttctacta cagagtttgc ggatgaggtg tgtaagtata ttgtatagat
     7561 tttatttagg gaaaaagtga aatatagatt gaaggaaaga ggctttctca aaatagagat
     7621 aagccttttt ttattggaat gataaaatat atttgatttt atacaaaaga gagttcctca
     7681 taaacgaaaa agttaatttt gggatatcca ttttaatttt aaaattaatt gattaatacg
     7741 caaagaaaga ttaaacaaat ttattatttt taattgaact ttcaaccaaa atatggtaaa
     7801 atttaaaaaa gttgaacttt caactaaaaa taaatatatt gattatgtga aattctataa
     7861 aacagaaact atttgacttt agtcatggta ggttcagtgg aggtgtgttt gattaagact
     7921 ttagatagta atatattaag agaagtaggt actttatcta gagcagtaaa ttcaataaat
     7981 gatattaagt acaaggaatt aaagttacaa aaaggacaat ttacattttt gacaagaatc
     8041 tgtgaaaatc ctggtataaa tcttgtggaa ttatcaaata tgttaaaggt agacaaggct
     8101 acaacaacca aggctataca aaaactaata aaggcaggat atgtagataa aaagcaggat
     8161 gagtttgata aaagaggata taatttaact ccaactgata aatctttgga agtttatgag
     8221 ttgataattg aagaagagaa tagaagtata gagatatgtt ttgataattt tacagatgaa
     8281 gaaaaacaag tggtaactaa gttattagaa aaaatgagca aaaacgtaga aaatgaatgg
     8341 tttaaagtaa agagatagta aataaataca ttaacttaag gagatatttt tatgattaga
     8401 aaagcgaata tgaatgattt agaaagcatt atgaaaatta taaaatctac agtggaagaa
     8461 atgaagactt ataataatac acaatgggat gaaaactatc cattagaaaa agactttgtt
     8521 tcagatatta agaaacaaga tttatatatt tatgaagtgg atggagaagt agcaggattt
     8581 atatgtctta attatgaaga gccagaagaa tatttaggct taaattggtc atctaataaa
     8641 aaagcaatgg taatacatag aatggctgta aatccaaact ttagaaagac aggaatagca
     8701 tctaagttag ttgattttgc tgaaaaatta gctgtagaaa acaatgtatc atacttaaag
     8761 agcgatacat attcaataaa ctcaaaaatg aatttattac ttacaaagtg tggttttata
     8821 aaaatcggag aaatgagttt tttaggaaag gagaaatctt tctactgtta tgataaaata
     8881 ttataaagag catttataat acaaggggta aataatagat tttcagcatt agtaagggca
     8941 taatataacc cagtatatgc tattatgaaa gggatgattt atatgattgg atttctaaag
     9001 aaaagaagaa gtattagaaa atacaaggat gttgaagtag aaaaagaaaa attagataaa
     9061 atattaaaag ctgctctttt agctccatca tcaaaggggc ttagaacatg ggaatttata
     9121 gtggtagatg ataaagaaaa gctaattaat ttatctcaat gtagaactaa gggtggtgga
     9181 tttttcttaa aaaatgcacc tttagctatt gtaattattg cagataaaga aaaaaatgat
     9241 gtttggatag aggatgcaag tatagctgct tcatacatac aacttcaagc tcatgaacta
     9301 ggtttaggtt cttgttggat acaagtaaga aatagaatgt atgatgataa tatagaagct
     9361 gataagtaca ttagagaaga attaaaagta cctagcaagt acagtgtaga atgtataatt
     9421 tctataggat attctgatga agaaaagaaa gcatacaatg actctgattt agactataaa
     9481 aaggttcatt ttaacaactt ttagtatata atatgttgta ttaagacatt atattttttt
     9541 ctattgttaa tatatactta aaattgtaaa aatatatctg aattttacta acttatctag
     9601 gggttatgta caaggatttt aaagataaat atagaaatta atgtttataa tcgatacata
     9661 acttgagtaa caaaatttaa ggaggttaaa gtatgggtat atttaaaaca aaaatggatg
     9721 aggactggaa agtaaactac attaaagagt ttaatgaaat gagagattct tatgagagta
     9781 aacttcaaaa aaaacagttt gaggtagata gtttgaaatc agaattggat agactaagaa
     9841 gctataaaaa tagccttaag ccaaaagaaa aacaaattac tgatgatgat ataaataata
     9901 taaagagttt aagaagagat ggcttaagct ataaagaaat ttcaaatcaa acaagttgga
     9961 gcaaagcaac tgtaagtagg gtattaaatg gactttacga ttagcatata catggggtga
    10021 tttaaattgg gatgaagtat agttacaaaa aatgcataat agattctatt ttattgatgt
    10081 ttataattca aatattaagg atgattttga attatgtact tctaagtcaa tttgaattta
    10141 ctcttgaaaa ttttaatatt ataaacttaa tttcttttac attagttggt ttatcgttaa
    10201 ttttattttt aaaagataat tctttgtata acaaggttag aaatagaaaa atcacagaag
    10261 cttttgaaga aaataaaaac aatatactta ttgaaaagtg taagcttata ctttttgttg
    10321 tggttttatc tttagcaata attgtaactt attgtactaa gggatatgta ctttttaatg
    10381 taactatgat gactttgtct gtattgatag ttccaatatt tgaagagtta ttttttagag
    10441 aatatatatg gaactatcta agtaatttta taaagtcaaa gagtaaaatt atctgtataa
    10501 catctatact atctggaatt tataatatag gatacataga tgttataaga aattatgtaa
    10561 tattgtataa caattcaagc tacacctttg aagttattat ttcaaaaatt atgataggta
    10621 ctgtttttgg tattgtactt ggattagtta agtataggtt tagagatgta gggttttgta
    10681 tattattgag gagcttattt gctatattta ttaggtag
//
LOCUS       DACTGW010000134        10699 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.134, whole genome shotgun sequence.
ACCESSION   DACTGW010000134 DACTGW010000000
VERSION     DACTGW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10699
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.134"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..336
                     /locus_tag="I9573_002525"
     CDS             <1..336
                     /locus_tag="I9573_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HAT4797135.1"
                     /translation="KVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKE
                     AGIDSEITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYY
                     GILKSVYGK"
     gene            469..1839
                     /gene="hydA"
                     /locus_tag="I9573_002526"
     CDS             469..1839
                     /gene="hydA"
                     /locus_tag="I9573_002526"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HAT4797136.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKN
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            1857..3263
                     /locus_tag="I9573_002527"
     CDS             1857..3263
                     /locus_tag="I9573_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4797137.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            3335..3781
                     /locus_tag="I9573_002528"
     CDS             3335..3781
                     /locus_tag="I9573_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HAT4797138.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            3820..4614
                     /locus_tag="I9573_002529"
     CDS             3820..4614
                     /locus_tag="I9573_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAT4797139.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            4604..6895
                     /locus_tag="I9573_002530"
     CDS             4604..6895
                     /locus_tag="I9573_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAT4797140.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDTKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            6896..8209
                     /locus_tag="I9573_002531"
     CDS             6896..8209
                     /locus_tag="I9573_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAT4797141.1"
                     /translation="MIAIKSKYLISSADEIYTNSAAVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            8245..9330
                     /locus_tag="I9573_002532"
     CDS             8245..9330
                     /locus_tag="I9573_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAT4797142.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            9560..10651
                     /locus_tag="I9573_002533"
     CDS             9560..10651
                     /locus_tag="I9573_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797143.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEMTVMKEDISEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEINVLKEDMSEVKQ
                     EMIVMKEDMSEVKQEMTVMKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDISEVRQEM
                     TVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNWSDIVKLKSVK"
BASE COUNT         4299 a         1120 c         2192 g         3088 t
ORIGIN      
        1 aaagtttctt atgcggttga aaaagctgat tgctatacag gaaatactat agaaagtgaa
       61 agattcttcc caggttggtt atatgacgaa gaagatgaat ttgttcaagc agcatataag
      121 ggattaaaag aagctggaat agattcagaa ataactcaat actcattctg tacgaatgga
      181 agtcactatg caggtgaagc tggaataaaa actataggat ttggtccttc taaggaaaat
      241 cttgctcaca caatagatga gtatatagag caagaacagt tatttatagg aacagaaggt
      301 tactatggaa tattaaaatc tgtatacgga aaataatatt tagttcacaa taaattattt
      361 aaatatagtc tagaggtgct gaatatagca cctttagaaa atacaatttt aaataaagtt
      421 ttacgatact tagaaaatac tttaaagctt aaaagttagg aggtatatat gggaacaata
      481 ctaagaggtg gaactataat tacctctgat aagacttata taagtgattt aaggatagaa
      541 gatgaaaaga tagttgaaat aggaaataac ttagaaataa atggtgataa ggttattgat
      601 gctacaggta aaattgtaat tcctggtgga atagacacac atactcattt tgatatgaat
      661 gcaggttcta ttacaactgc agataatttt gaaacaggaa caagagcagc tatagctgga
      721 ggaactacta caatactaga ttttgcagaa gccaatgagg gggaaaacct actgcaaggt
      781 gtagaagcat atcataaaaa agcaagtgga aattgttatt gtgattatgg ttttcatatg
      841 acaattacat gtttaaataa agatacattt aatcatatgg aaaaacttat ttcggatggg
      901 atagtatctt ttaagatgta tatggcatat gatggcatga aagtagatga tggaacaata
      961 tacagggttt taaagaaagc aagggattta ggttgtatag ttggatttca ttgtgaaaat
     1021 ggggatttac tagatgtatt gatagaagaa aatatacaaa atggtaattt agagccaaaa
     1081 tatcatccac ttacaagacc taacatagtt gagaaagagt ctgtttcaag acttgctgat
     1141 ataactaaat tatctaattc aaaatcctat gtagtccact taagttgtag ggagtcactt
     1201 gaaactgtaa aaatggctag agaaaaaaat atagatatga tagtagaaac atgcccacag
     1261 tatctattat tagaagacaa tttatataat aagaataaat ttgagggtgc taagtatgta
     1321 atgtctcctc ctttaagaaa aaaagaagac ataaattacc tttgggaagg attgtctagt
     1381 ggagatattc aaacagttgg cacagaccac tgttcattta attttaaggg gcaaaaagac
     1441 ttgggaattg atgattttag taagatacca aatggagcac ctggtgttga acataggtta
     1501 gcacttttat atacatatgg ggtcttagaa aatagaataa gtgtaaataa gtttgtggaa
     1561 gttacttcta cgaatgctgc aaaaatattt ggaatgtatc ctaaaaaagg tgagatagct
     1621 gtaggaagtg atgcagatat agtcatttta aatatcaata aagaagaaac aattagttat
     1681 aaaactcaaa aacaaaatgt agattatact ccatatgaag gatttaaggt taaatgtaaa
     1741 gttgaagatg tatttttgag aggaaatcat gtagtacaaa gttgtaatgt taaagaacat
     1801 ccaacaggtc aatatattaa aagaaaaatt actaaataat gcggggggaa ataagaatga
     1861 tggatatact tattaagaat gccataatag taactgtgaa taaagagaga gaagttattt
     1921 ttgatggggc attagtagta aaagataaca agattgctga tattggaaat tctaaagaga
     1981 tagaaagtaa atatactgat gttaaaaaaa ttatagatgc taaaggtaaa gttttattcc
     2041 caggttttat aaatacacat aatcatttat tccaaacttt attaaaagga ttgggagatg
     2101 atatggtact taaagactgg ttagaaacta tgacatttcc agcagctaac tatttagagc
     2161 caaaagatac ttatgatgct gcaatgcttg gatgtattga aggacttaga agtggtataa
     2221 cgaccatggt tgactacatg tatcctcata gtaagccagg tctttgtgat ggaataatag
     2281 atgcttataa agaattagga ataagaggaa tacttggaag aggatgtatg aatacaggag
     2341 ctcaatttgg agttcatcct ggaataatgc aagatgtaga aacggttgaa aaagatgtaa
     2401 gaagattgtt tgaaaaacac cataatacag aaaatggaag aatcaaaata ggagtagctc
     2461 ctgcagcaat atggtcaaat tctcaagaaa tgcttgaaat gctatggaga gtggtaaaag
     2521 aatatgatga tgctttattt acagtacata tatcagagac tccttttgat agagaagcag
     2581 caaaagaatt gcatgggcaa tatgacatag atgttttaga aaaattagga atacttggac
     2641 caaatgtgct tatggtacat tgtgtgtatc taactgaaaa agatatggaa cttactaaaa
     2701 aatacgatat gaaagtgtct cataatacag caagtaatat gtacttatct tcaggtgttg
     2761 caccagtgcc agaaatgctt aaaaaaggaa taacagttag tttaggagta gatggagccg
     2821 ctagtaataa ctctcaagat atgcttgaac ttatgaaatt aacagcactt caacataaag
     2881 ttaataagtg tgacccatta gcaatgtcgg cagaaaaagt tcttgagttg gcaactatag
     2941 atggagcaag agctattgga atggaagatg agataggttc attggagata ggtaaaaaag
     3001 cagacttact aatatttaat ccaatgcttt caccaaaagc gatacctatg cataatccgg
     3061 tatctacatt agtatattct tctagtatga aaaatataga gagtgttata gtagatggaa
     3121 atataataat ggaagattca aaaatattga cagctaatga agagaaagca ttaaaagatg
     3181 ctcaagatac agctgagaga ctttgtgtaa gaggaactat aaaaaataga atggaaggtc
     3241 ataaatggaa tagcctttat taataaatgt aatttattca tagaaaagta tagtaaaact
     3301 ttagcttaaa aatatgagtt ttgaggtgat ttatttgatt atcaaatgta ccataaataa
     3361 taaagaaaaa gaattaagta taagaccaga tgatttttta gcagatacat taagagaaaa
     3421 tggatatgta agtgttaaaa aagggtgtga tacaggctca tgtgggttat gtacagtgat
     3481 ggtaaatgac aaggccgtgc tttcatgtaa ttatcttact gctagagtag atggtcagca
     3541 tattacaaca cttgaggggg tcttggatga agcaaagaag tttggagatt ttcttgcaga
     3601 cgaaggggct gaccagtgtg gattttgtgc tcctggattt ataatgatgg ttttggcaat
     3661 gaaaagagag cttcaaaatc caacagaaga agaagttata gattatgtaa atggtaacct
     3721 ttgtagatgt acaggatata aaggtcaatt aagagcaata cataaatatt taaacactta
     3781 gacttttaag tgaaaattaa gttgatatgg ggagagagta tgataactat aaaggaatat
     3841 gtagttccaa aaagtttaga tgaagcttat gaactgctaa tatcaagaaa aaacaacata
     3901 atacttggag gatgtggatt tttaaaacta ggtagtaaaa atattggctc agcaatagat
     3961 ttaaaagact tagctttaga ttatataaat gaaactgatg atagcatatt aattggagca
     4021 gacacaagtt taagaacttt agaattaaat aaggttatta aaaattactg taatggggta
     4081 atttcaaacg ctgtttctaa tatagtagga gttcaattta gaagtggagc cagagtagga
     4141 gctagcgtat ttgctaagta tggattttca gatttaatac cttcattact agtggtagat
     4201 gcaaaggtta agttatataa aaaaggggtt atgaacttag ctgatttttt agaaagtgag
     4261 cttgaaaaag atatattaat agaagttata ttacctaaaa aagatgctat aggtgtattt
     4321 gattctataa gaaaatgtac aggtgatttt gcagtattaa atggagcaat gttaaaagaa
     4381 ggaagtattt ataaaattgc gataggcgct agaccaagaa gagcaaaaat tgcctataaa
     4441 gcaagtgaaa tattaagttt agaaaatgat atagaaaaag ctggtgaagt atcaagtgaa
     4501 gaactttcat ttggaagtaa tataagagga agtaaagaat acagaaaaga tatggcaaaa
     4561 gccttagtag tgagaatgta taatagtatt ggaggtgagt gtgatggtaa ataaagaggt
     4621 aagaaaaata gatagtgaag ccattgttac tggaaaacca atatatacag aagatttaat
     4681 ttttcataaa gatgttttaa caattaagct tcttagaagt ccgcatgctt ttgcgaagat
     4741 aaaaaacata gatacaaaaa atgcattaaa ggtaaaaggt gtagtaaaca tattcactta
     4801 caaagatgtt ccaaattata gatattcaat ggttggagag tcatatccag aagcatcacc
     4861 tcatgaccaa ttaatacttg aagacgtagt tagatatgta ggagatgaag ttgctataat
     4921 agccgctgaa gatgaaaggg ctgctattaa agctatgaaa cttataaaag ttgaatatga
     4981 agtaatgact cctatattgg atgcaagaga atcggaagga aataaaattt taatccatga
     5041 agaagatgat ttgttttgtc catttgattt tggattggat gctacaaaaa atattgtatc
     5101 gagagaaaaa atgggtaaag gggatgtaga aaaagagttt aaagaaagtg atgtaatagt
     5161 agaacattct tatatgactc aatcacaagc ccattgtatg atggaaactc atagagctta
     5221 tacgtatata gatacgaatg gcagattagt agtaacttct gcaaaccaat cagcttatca
     5281 tatgagaaga caggtttcta aagcactggg aatacctctt tcaaaagtta gagtaataaa
     5341 accaagaata ggtggaggtt ttggtggaaa aaacatagct gtaacagaga tatatgcatc
     5401 atttgtaact tggatgacaa aaagaccagc aaaactaatt tatactagag aagaaacttt
     5461 tgcaatgact aatactagac atcagatgtt ttttgatata aaaattgggg cagataaaga
     5521 aggaaacata aaagctatag atatgaaagc attgaataat acaggcgctt atggtgataa
     5581 tggaccttca gtttgctctg aatcaggtca taatgtactt ccaacatata ataatgtacc
     5641 tgcaataaaa tttgatggga gaactgtata tactaattta gttccaggag gagcattaag
     5701 aggatatgga gcaacacaag gtacatttgc acttgattgt gcaattgatg aattagcgca
     5761 taaacttaat atggacccaa cagaaataaa gttaaagaat attataaaaa aacaaactga
     5821 aggcggaata atgaattttc caataagaag ttgtgcactt gatgaatgta taattagagg
     5881 taaaaaactt attggatggg atgaaaagta ccctgttagg gatattggaa acaataaggt
     5941 gagggctgtt ggaatgtctg tgggaactca tggttctggt atagccaaca tagacatggc
     6001 aatagtaaat atgaggatgg atgaagatgg aacttataag ttgttttctg ggtcttcaga
     6061 cttaggaact ggttctgaca caatacttgc acaaatagct gcaaaagcat tgaatacatc
     6121 tattgataga atttccgttt atacaggaga tacagatatg tgtccatatg atactggtgc
     6181 atacgcttct tgtactactt atgtaactgg aaatgctaca ataaaatctg ctgaaagttt
     6241 aaaacaaaag atattaaaag tagcacagaa gaaactcgat ttaccaatag atgatttaat
     6301 actatatgaa gacagagttt gttatagtga agatgtagag aagtttgttt tgttaagtga
     6361 attaggaaaa gaatctgttg gtggtggaaa tcaagaagtt ttatattcat ctgaatctta
     6421 tggtatacaa gagagtgcta aaccattctt agcaggattt gctgaaatag agttagataa
     6481 aacaacaggg gagtttaaag taataaatta tgcttgttct acagattgtg gtacagttat
     6541 gaatcctgca cttgctagaa tacaagttga aggtggtgta actcaaggta tagggcttgc
     6601 aatgtatgaa gaccctagac atggttctga tggaaagctt caaacttcta atctattaca
     6661 atacaaagtt ccaacaaaaa aagatatagg aaacataatt gttgattttg tagataatta
     6721 tgagccaaca ggtccatatg gagctaaatc tttaggagaa atagttattc atacacctgc
     6781 accagcaata gcaaatgcaa tatacaatgc cacaggagtt cgtataagag aacttcctat
     6841 aacttctgag aaagtttata tggccatgaa aaaattaaat cagggaggta aataaatgat
     6901 tgctattaaa agtaaatact taatatcttc tgctgatgaa atatatacaa attctgctgc
     6961 tgtagtagaa aatgacatta taaaagatat attgccaaat gaagaagttg aaataaaata
     7021 taaaaatatt gaagaaataa tagataaatc agatgctata ataatgccag gattcgtcaa
     7081 tggtcatatg catcaatatg gagttctttc taggggaata ccggcaaatg tacattttac
     7141 tgactttgaa ggttttttaa atgactattg gtggccattt atagaaaata gaattggatt
     7201 gaaagaagtg aaggcaacaa ctaaagcatc agcaatagaa cttatagaat ctggtgtggt
     7261 agctttttgt gataccctag aagctccaaa tacagaagaa ggaactttaa ttgagcaagg
     7321 aaaaatacta gaagaaatag gcatgaaagc gattttatcc ttagaaagtt gtgaaagaat
     7381 aagctttgaa aatggactta gatgtttaga tgaaaattct aatttaataa agtggagtag
     7441 agaaaatagt aaattaataa atggaataat gtgtacacat acaagcttca catgttctga
     7501 tagatttatt aagaaagcta aagaagatgc aaaaaaatta aatgcaccaa tgcaatttca
     7561 tttatgtgag agtatatatg agccaaacta tgctgaaaaa cattttggca aaaaagcagt
     7621 tgactactat aatgatttag atatattgga tgaaactgta ttagcatctc agtgtgtaaa
     7681 agtaaatgat gaagaaatag atatattaaa agaaaaaggt gtaaaagtgg ttcatatgcc
     7741 tcttagcaac tgtgaagtag gtggaggttt ttctccagta ccaaaaatga ttaaaaaagg
     7801 cataaaagtg gcactgggta ctgatggata tataaatgat ttttttgaag ttatgagagg
     7861 tgcattttta atgcataaat cagttgaaga agatgcatca gtaatgccag caaatttagt
     7921 atttaggatg gcaacagagc atggtgctta tgtattagga cttcaaaatt ctggtaaaat
     7981 agcagttggt aataaggctg atataatagt tatggaagat gaatttaaaa ctcctgtgac
     8041 tcttgataat atatttgacc agatagtagt tcagggtaaa aaagaattta tatcaaatgt
     8101 atacatagat ggtagacata ttttaaaaga aaaacagtta gttgatttag ataaaaaagc
     8161 tattgttaaa gaaatgaaag aagttgcttg tgaattttgg aaattttaac gaatgtaaat
     8221 atatatgaaa aggggaagca gataatgtat aattttttag gtaaagaatt aaaatcacca
     8281 cttataatag gttcaggacc attaacatat agtgcagctg gatgcaagat attaagtgat
     8341 gcaggagcag gggcagttgt gactaagact ataagaaaag aaagagctat aaatccagca
     8401 ccacatatgg ttagaaatac agctaatgct cttttaaata atgaaaaatg gacagatttt
     8461 gagccagagc aatggataga ttttgaaata ccacaaatga aaagagatgg gactgtctgt
     8521 atagcttcag taggtcatac aattgaagaa agtagcgaat tagtagagaa agttgcaaat
     8581 gcaggagctg actttattga attagtatct tatgactata gagatttgat accaatgctt
     8641 aaagatgcaa aggaaagagt aaatatacct gtaatagtta aacttcctcc tatgatagat
     8701 gagataggcg attttgctaa aaaattagaa gaagcaggag cagatgcaat aactgcttgt
     8761 gactctgtag gaccagcatt tagaatagac atagaaacag ggcaaccttt attaggtgga
     8821 aatggaatag gatatttaag tggagaaact attaagccaa taactttaca gagaatatat
     8881 gaaataaaaa aacaagttaa tataccaata attggcttag gtggatgtgt aagtggagat
     8941 gatgccttag aaatgattat ggcaggtgct gactttgttg gtatatgttc agtagtaatt
     9001 ttaaaaggcg cacaagtaat atctaaaata catgatgatt taaaatctaa tcttaataga
     9061 ttaggatata atactataga aaatgcttgt ggtatagtac ataatcatat gggagatgtt
     9121 aatgaaagac ttagctttga gtttacttat gatgaagaaa aatgtactaa atgtaagatg
     9181 tgcgagagag tttgtgcata tcaagcgaga aaattgaatg ataagatgga attgaatgaa
     9241 gaagagtgta gatattgtgg actttgtata tcagtttgta aaccaaaagc acttggaaga
     9301 aaaatttcat gttcagagct gaatgtatag attattttat aaaaaacaaa gttttaaata
     9361 aaaataagaa aggctatctc aaagagataa gccttttttg ttgttgacct tatttttgtt
     9421 tatttgtagt tattttagat attaattagt tttgaagtaa taaagtgaat caattttgtt
     9481 taattaaaat tttaataatt ctgatatata tgttaacatt gtatagcatt tgtgtaaaat
     9541 gttataataa ttaataggga tggtgatgca agtgcaagag aagattttag agttattata
     9601 tactataaat gaagatgtag ttagcataaa aaatgatatg tcggaagtaa aacaagaaat
     9661 gactgtaatg aaagaagata tatcagaagt aaaacaagaa atgactgtaa tgaaagaaga
     9721 tatgtcggaa gtaaaacagg agataaatgt attaaaagga gatatgtcgg aagtaaaaca
     9781 agaaatgact gtaatgaaag aagatatgtc ggaagtaaaa caggaaataa atgtattaaa
     9841 agaagatatg tcggaagtaa aacaggaaat gattgtaatg aaagaagata tgtcggaagt
     9901 aaaacaggaa atgactgtaa tgaaagaaga tatgtcggaa gtaaaacagg aaatgactgt
     9961 aatgaaagaa gatatgtcag aggtaagaca agagataaat ataatgaaag aggatatatc
    10021 cgaagtaaga caagaaatga ctgtaatgaa agaagatatg tcggaagtaa aacaggagat
    10081 aaatgtatta aaagaagata tgtccgaagt aagacaagag ataaatataa tgaaagaaga
    10141 tatgtctgaa gtaagacaag aaatgactgt aatgaaagaa gatacatcag aggtaaaaca
    10201 agagataaat gtaatgaaaa aagacatgtc agaagtaaga caagaaataa ctgtaatgaa
    10261 agaagatgtt tccgaagtta aatcagaagt aaactttatg caaaataaaa taaataacat
    10321 taatgaagat atgaatggaa ttaaggacga ggtatccata gcaaatgaaa aattatatgg
    10381 aattgaaatt aagattgata gtctagaatg tgaagataaa tcaatgaaag agatacagat
    10441 agaacagaat aatattttag gaagtttatt acataattct gaaataaata aagctactca
    10501 tgataatata gaacataaca tagcatatat aaaaggtgac actaattcta taaaagagga
    10561 tatagctgaa ataagaagag acttaaattt agtagagtta gccactagta aaaattggag
    10621 tgatattgtc aaactaaaat cagtaaagta gtattttaat tcactaattt atcatccaca
    10681 aactaagaat tttatataa
//
LOCUS       DACTGW010000135        10621 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.135, whole genome shotgun sequence.
ACCESSION   DACTGW010000135 DACTGW010000000
VERSION     DACTGW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10621
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.135"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            89..1360
                     /locus_tag="I9573_002534"
     CDS             89..1360
                     /locus_tag="I9573_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4797144.1"
                     /translation="MRSDWPKKFTENFNEQIIFIDNKPQVKQSGLEKLRENQLWLQIL
                     DSSGNRVYGFLEPKEHKDYYSNSELLTLANQNNSSNGTVYFNTIKNNKNDYIYIIYFP
                     LNISKVNMFFDGSKFVGGKSIVFSIIGIIFLIILISGIAYGYWVTKCISNITSAVSDI
                     SKRSYLPVNIKGVFADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLS
                     PIKGYAELLTDSSTNYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKN
                     KYDLVRFLKELSIDILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTY
                     GDKNTKVCVHITLNERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLA
                     ITKQIIEVHGGNISVESIPGLGSSFYITFPI"
     gene            1580..4057
                     /locus_tag="I9573_002535"
     CDS             1580..4057
                     /locus_tag="I9573_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAT4797145.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDVLTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIENVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDVVMIQIRDLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKKRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            4072..4758
                     /locus_tag="I9573_002536"
     CDS             4072..4758
                     /locus_tag="I9573_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797146.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALVGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            5031..5864
                     /locus_tag="I9573_002537"
     CDS             5031..5864
                     /locus_tag="I9573_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4797147.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCMQLKNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMYICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            5902..6363
                     /locus_tag="I9573_002538"
     CDS             5902..6363
                     /locus_tag="I9573_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HAT4797148.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            6516..6941
                     /locus_tag="I9573_002539"
     CDS             6516..6941
                     /locus_tag="I9573_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HAT4797149.1"
                     /translation="MHDELKIKMYSFTMDCKDPHELAKFYAALLNWEIAFYNEEYACV
                     GAPGTNQGTYPGITFQRNSEYKPPVWPEKPESQQQMAHMDFAVNDLEEAVQYEIHCGA
                     TIAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            7394..7714
                     /locus_tag="I9573_002540"
     CDS             7394..7714
                     /locus_tag="I9573_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HAT4797150.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            7852..8496
                     /gene="trmB"
                     /locus_tag="I9573_002541"
     CDS             7852..8496
                     /gene="trmB"
                     /locus_tag="I9573_002541"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAT4797151.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            8640..9371
                     /locus_tag="I9573_002542"
     CDS             8640..9371
                     /locus_tag="I9573_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797152.1"
                     /translation="MNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDANLRAVV
                     KRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEVPKEAL
                     SLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHNVDIAK
                     GESIDLRKIITKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGNKTGQA
                     VVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(9500..9778)
                     /locus_tag="I9573_002543"
     CDS             complement(9500..9778)
                     /locus_tag="I9573_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797153.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            10053..10499
                     /locus_tag="I9573_002544"
     CDS             10053..10499
                     /locus_tag="I9573_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797154.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
BASE COUNT         4106 a         1137 c         1654 g         3724 t
ORIGIN      
        1 ttttttcatg atttttattt gcttaatcct gacagtaata ggtattttct tatcacttat
       61 tactattcag aaaccaaatg gggaatttat tcgtagtgac tggccgaaaa aatttacaga
      121 aaactttaat gaacaaatca tatttataga taataagcca caagtaaaac agtctggatt
      181 agaaaagctt cgagaaaatc aattgtggtt gcaaatttta gattctagtg gaaatagagt
      241 ttatgggttt ttagaaccaa aagagcataa agattattat tctaattctg aattattaac
      301 attagctaac caaaataatt caagtaatgg aactgtatac tttaatacaa ttaaaaataa
      361 taaaaatgac tatatttata ttatatattt tccattaaat atatctaaag tgaatatgtt
      421 ttttgatgga agtaaatttg taggtggtaa gtcaattgta ttttcaatca tagggattat
      481 atttttaata attttgattt ctggaattgc ttatggttac tgggtaacaa agtgcatatc
      541 aaatattaca tctgctgtta gtgatatttc aaaacgaagc tatcttccag ttaatattaa
      601 aggtgttttt gctgatatat ataatagttt aaatatttta aatgaagaaa ttcatagaag
      661 tgatgagata caaaataaaa ctgatattat gagagaagag tggatttcaa atataactca
      721 tgatttaaaa actccacttt caccaataaa agggtatgca gagcttctta cagatagtag
      781 tactaattat tcacctgaaa aagtatataa atatgcttca atcatgttaa agaatataga
      841 atatacaaat acacttataa acgatttaaa attaacctat caattaaaaa atggaataat
      901 tcctttaaat aaaaataaat atgatttagt aagattcttg aaagaattat ccattgatat
      961 tttaaattat ccagaatatg aatcaaatat agttgaattt aagagtaata tagataatat
     1021 taactttgat tttgattgta cactattaaa acgagctttt agtaatctta ttgtcaatgc
     1081 ttttacatat ggagataaaa atactaaagt ttgtgtacat ataacattaa atgaaagaat
     1141 tgaaatcatt atatctgata atggaaaagg gatgtcaaga gaagaagcat caaatttgtt
     1201 taatcgttat tatagaggaa gcaatacaaa acaaaaaata gaaggaactg gtttaggact
     1261 agcaataacg aagcaaatta ttgaagttca tggagggaat atatctgttg aaagtatacc
     1321 tggattaggt agctcatttt atatcacctt tcctatttaa ttaaggttaa ttaaggtcaa
     1381 ataaaaaata tattaaggtt gatagattat tatatttata tagtagttta tcaatctttt
     1441 ttatttaagg aggtgatttg attaaaaata ttataaatag aatgataaaa gtatggagga
     1501 gtctattaga gattcaattt ggattaaagt ataaaaatat tatactgaaa tatcttagat
     1561 aaaggaagaa ggtgtaaatt tgaaaattat attaaagtat attttcacaa atattaaaga
     1621 acgaaaaatt agaacagcag ttatgttact gtccatagtt ttatcaacag ttttactttt
     1681 tgtttcattc tcaattggat tatcttatga aagtgcacaa agaaaaatgg caaaaggtat
     1741 gtatggtaca gcgactatat ctgtccaaag taaaaatcca gatgtattaa ccaatttaga
     1801 agacatacct gatttaaatg ctataaaatc aaaggttggt gtattggaaa gctctgctat
     1861 ttataataaa gggggatact atgaagaatt tagtcttata tcagcagact taagtcaact
     1921 aaataaaatc aacaagccac gattagaaaa tggcgatagt attactgatt tttctggtga
     1981 taaaattatt cttccaaatc gctttacatc aaaatataaa ataaaaaaag gtgattctat
     2041 tactttacag atttatggaa agtcttatac ttttcaagtt agtgatattg catcttatga
     2101 tacagtattt ttgaggaata caagaggtgt aaatgcactg ctacccaaag agactctatc
     2161 aaaaattata aataaaggca gtggatatac aagagttctt atagagtcag aagaagaaat
     2221 gacagaaaat ctagtaaata aattatccga agaactttcc actgaaaaat atatggtatc
     2281 aaatactata aatgaaacta agataatttc agatgctaga caaaagacaa tgcctttctt
     2341 tttaataagt ttttttgcat taacccttag tatatttatc atatacagca gctataaggt
     2401 tattacatta gaacgtcttc cttttattgg aacatttaga agtattggtg ccaatgaaaa
     2461 aactgtaact cgtatattga tgcttgaaag tatattatac ggaagtatag gaggattaat
     2521 tgctattcct attggtgtag ttgttttaaa tttaatgctt catgggttag gaaattctct
     2581 cgaacaagga atatcaattc cagtagtaat atcacccatt ggagtgatta tatctgttat
     2641 agtagcaata atcgtttctt catttagtgc atatatacct gttaaaaaag ctagccattt
     2701 gccaataaaa aatattgtac taggaacagt agaagaaaag aatgtttcaa atcgttctat
     2761 actttttata ggaagtataa tgtttatttt atctatttta cttccaagaa tatcccctga
     2821 aaatacattg tatctagctg gtggattttc attactagga ttaattgtag ctactattgt
     2881 gttgattcct cttattactg atatcatgtc tatagttttt gagtttgttt ataaaaatat
     2941 tttagggaat gagggtaaat tagctgctag aaatatgaaa aataacaaaa atataataca
     3001 gaatatcact ttactattta taagtatttc tgcagttatt gcaataagtg ttgttggtaa
     3061 ctttgtcaaa acttatataa cagatgtgtt tcgtgacgca gaattacaag gttttgcaga
     3121 tggaaagatg aatgaagagt ttattgaaaa tgttagacat atggatggaa ttaaaaaaat
     3181 attacctctt tatgttatga ataatgagat ttctggtaat ggtgtaacat tatcaagact
     3241 agagggtaca gataatataa aactatataa ctctatgttt ggaatcaatt atacaaattt
     3301 tgagataaaa aaacaagtaa tagaagcttt taatgataaa cgttctgtta ttttgaatga
     3361 agatactcta aaaaaagtag gcttatctat aggagataca ataactctat ctaatgataa
     3421 gtatgatttt tcttataaaa tagtaggtag ttttaaatcc cgtgcaaatg atgtagaagc
     3481 tgttatacca tcacattatg cagttaatga ttttgataaa actaattatg gttttttagt
     3541 atatacagca gtcaatccag atgttgttat gattcagata agagatttat ttggagatac
     3601 atataattgg agtcgtacag ttgaagaatt taataatgat tctttaaata ctataagtag
     3661 ttttttgtct cctatgaata agatgaccta tttcattttt cttcttgcta cagtaggtat
     3721 tattaataat ctactgatta attatattca aaaaaaacgt tctatagcta tgtataaatc
     3781 tataggacta agtgataaac aaaatataaa agtgacattg attgaagggt ttacttcagg
     3841 tttacttgga gctgttattg gtattgtgat atctatactt gagattcaaa ctatctttat
     3901 agttgcagga ccaaaaattt caatgaaacc agatttagat tttaaaacat ttattattgt
     3961 tgggttattg ggaattatag taactttaat aggttcgata gtgcctatta taaaagggaa
     4021 gaaaatgaaa ctgatagaag aaattaagtt tgaataatta tggaggttaa tatgaaaatt
     4081 acacataata gtttagctgt agaagcaaaa aatattatca aagaatataa aattggaaat
     4141 acaacaactc gagtgttaaa agaggtatct ttacaagtaa tgaaaggtga atttgtttct
     4201 atcatgggtc aatctggttc tggtaaaagt actcttttat atattttagg tggtttagat
     4261 actccaacga gtggaaaagt gtatatgaat ggagctgata tatcacattt taatgatgag
     4321 aaaatgagca taatacgtcg tagaaatata ggattcgtat ttcaatttta taacttaata
     4381 ccaaatttaa atgtggagga aaatataatg cttcctttgt tattagatgg taaaaattta
     4441 aaagattata aaaatcaatt agatgaaatc ttagatatag ttggtttaac tgatagacgt
     4501 aaacatacac cacgagagtt atctggtggt cagcaacaac gtgttgctat agctcgagct
     4561 ttagttggaa aaccagagat tttatttgct gatgagccta ctggaaattt agatagtaaa
     4621 acagggatag aaataataga tttactgaat aaaataaaca gagacaatgg acaaactata
     4681 attatggtaa ctcactcacc agaagcagcc aaaagtagta gtcgtactat tacagtttca
     4741 gatggtttaa ttgtataagg actaattatt gtatttgaga taagcatttt taactatata
     4801 aattagacat atgtaaagtg gatataagtt aaatactata aatgtgctat aatattaaaa
     4861 taacagataa aagtactata ttttttattg attattttaa tttatttgtt tattaaatat
     4921 ctaatgagta ttcatactat ctctattatt aaaagcagtt aatgatatat agagataata
     4981 tggattcttc ttactttaat aaatattcac gataggacag gatataatat atgttaaaaa
     5041 aagaaagtat tcattttata aataaaaata ggtcacttat agtttgtgaa gacaattttg
     5101 tttatctctt gccttgtatg caattaaaaa attggatatc gaattttact atatcgtttc
     5161 caaatcaaac aataatttct gataattaca cgattattcc tcatggaagt gtaactttgg
     5221 tgttttttta tgatgccaca gggttacata gtctattatt tggtccaact actaaaccaa
     5281 aaacagtagg aaatattgct aatagatgtg atgttatact aattatagaa tttcaacctg
     5341 ctggtttttt cccgcttata ggtatacagc aaagtgaact tattgataaa gttgttccat
     5401 tttctataat taatacttca ttagatttag aaattaaaaa aatatttaat gaatctttat
     5461 ctatagataa attgatttta aagttggaag aattattgat ttctaatatt aaaattgaat
     5521 actcatatga gtttattttg gcaattcagt tgattattca aaactctgga aacatatctt
     5581 cacaagaaat ttctaaaaaa gtattttata gttctaggca tttaaacaga ctctttaatc
     5641 agtacctagg gttgagtatg aagtcatttt ctcgtttggt acgtattaat aaatctataa
     5701 aacttctaaa caataataaa acttcattga tgtatatatg taatgaatta ggattctatg
     5761 atataccaca ttttattaaa gattttaaaa ttgtctgtgg aataactcct caaaaatacc
     5821 gagctaatat gtctgatttt tacagtgaaa ttgcaaaatt ttaagttata atacttataa
     5881 caaagaaagg atagtgataa gatgaaattt ggaggagtat tgctaattgt caaagacata
     5941 aataaatcaa aaaagtttta tgaagatgtt atggagcaaa aaattacatt agatttagga
     6001 gaacatgtat catttgaaaa tggatttgga ttacaaagta attataaagc tataattgga
     6061 tatgatttac caatatatca acaatcaaat aactttcagt tgtattttga ggttaatgat
     6121 atcaaacaat gggaaagtaa gataaataga ataggaaata ttgaattttt gcattatata
     6181 aaagaatacc catggggtca aagaactttt aggttttatg attttgataa aaatattata
     6241 gaaatttcag aaagtatgga aagcgtcata aagaggcttt taaaacaagg tttagcttta
     6301 gaagaaattt caaaacgcac tatgtatcca gttgaattta ttacacaatt tcaacaatta
     6361 taaaatatta aaatatataa ttttttcttt atattgcaaa aaacaaaggc ttggggcata
     6421 atagaaaaat acttgaaata aattacatac ttaatgtttg aatttagctt aaaaatgaaa
     6481 agacgaagaa atttatactt ggaggtaatt acgttatgca tgatgaactg aaaatcaaaa
     6541 tgtactcatt tacgatggat tgcaaagacc cacatgaatt agcaaaattt tatgcagcgt
     6601 tactcaattg ggaaatagca ttttataatg aagaatatgc atgtgttggt gctccaggaa
     6661 caaatcaggg aacatatcct ggtataacat ttcaacgaaa ttctgagtat aaaccgcctg
     6721 tgtggccaga aaaacctgaa tctcaacagc aaatggctca tatggatttc gctgttaatg
     6781 atttagaaga agcagttcaa tatgaaattc attgtggagc tacaatcgca gaggagcaat
     6841 ttactgatga ctggagagtt atgattgacc cagctggaca ccctttttgc ttatgtcaga
     6901 tgaaatcaat tatggaaagt agccattttt cattgttata gaccaaaata ttgtttatta
     6961 attacaaaaa agcacaacag tatataccgt tgtgctttta cttattatac atgaatacac
     7021 taaaaaatat aaatagtaga gctttatcaa taatgataat ttttaaaaaa tagtattttt
     7081 ttattgaata atttcttaga tattttaaag tatataaatt ttgcatatat gaattggaca
     7141 ttatcaaatg tgttataata ttaaaacaac aaataaaatt attatatttt ttagaataat
     7201 aattttattt gtttactaag tttatgatag ggtatttatt taaggtaata aaatattata
     7261 taaaatattt taaagcctta ggtaatatta ggtagaagtt ttagtaaaat atgcaaaaac
     7321 taattgatta tgtaccttgt atgggtatat aatagatatt gttttaataa aatatttgaa
     7381 agggaatgat ttgatgaaaa ttactttacc agaaacagct attgatactt taaaaagtat
     7441 cttaaaagac aatcaagata aacctaataa tataagagta tactttgcag gtgttggttg
     7501 tggaggtcct tcttttggat tagctttaga tgagaaaaaa gaagatgact taacttatga
     7561 agttggagaa ttacaatttg taatgagctc tgatgaatat agtcagtatg gagatataat
     7621 aatagaagat acaggatttg gatttagagt tattcctgaa aatatgaaag atcaaggtgg
     7681 aggaggatgc tctggttgtt cagggtgcca ctaagattaa gtttttagta agtttagaac
     7741 tctctttata gaaataaatt tctattttaa gagagttctt tttatttttt ggtgagataa
     7801 tgtataataa aaatgtattt tactaatttt ttatatgtaa ggagaattat tgtgagaaga
     7861 agacgaaaaa aaggtgcaga tgaaaagctt ttaagttata caaaatatgt tttaagagac
     7921 gatatagaca aattaaaagg caagtggaat ttaaagttta gaaatgataa tccaatacat
     7981 gttgaatttg ggactggaaa aggtaagttt ataacaactt tagctaagca gaaccctgac
     8041 ataaattata ttgccatgga acttaaggaa gaagttcttt taaaagctgt tgaaaaagca
     8101 gatgcttcta atttgaataa tatactattt ttatggggag atgtaagcaa tattttggat
     8161 tactttgaag ctaaagaatt atcgagaatt tatataaatt tctgtgaccc atggcctaaa
     8221 aatagatgga gtaaaagaag actcactcat tctggatttt tagaaatgta caatagggtt
     8281 ttggaagatg atggcgaaat tcactttaaa acagataatg aaaaattatt cgagtttagt
     8341 ttaaatgaaa ttgctgcaaa taattggcta ttaaagaata tttctcttga tttaggaaat
     8401 agtgagtatg aaaataatgt tacaacagag tatgaagaca aatttatgtc acaaggtatg
     8461 cgaatattta gatgtgaagc taaaaaacgt aactagtaat atgtttataa gtttaatagt
     8521 agaaatttat tttaatttat attaataaag tgtacgaaag taggtatata taacaaggaa
     8581 acagaaccct ctcatagaaa ttaaattatt ataataaaaa atttatgtgg gagggtacta
     8641 tgaatttagc taatttaata ccatttatta tttctgcagc agctttaagt ccaataaatt
     8701 caaatgataa ggttttgagc gaaaatcaac ttaaagatat ttttcctgat gcaaatttaa
     8761 gagcagttgt aaaaagatat ataaacccag atgagatgac tataagcaat ataaaggctt
     8821 tagatggaga attttatgca actggtgaga gcatttcaaa tttaaaagga atatcatatc
     8881 ttgaaaatgt agacaatttt attttttgga ataataatat aaaagaggtt cctaaggaag
     8941 ctttaagtct aaaagatatg gattcaataa atcttgccaa taattatttg attgatgacg
     9001 atgtagtaaa ttctctttct cataatggtg tggatgtaaa ttgcgattta aattttattg
     9061 atacaaaaga taatcagtat aaactggact ctaaatatca taatgttgat atagcaaaag
     9121 gtgaaagtat tgatttgaga aaaataatta cgaagaaaat tgatagttat tataaatact
     9181 gggaagttac tgataatctt ccaaaagatt tagactttat tgtatctgta agtgataaga
     9241 gtgtactttc atgtgaagat atgataatta aaggtaataa aacaggtcaa gctgtggtaa
     9301 aagttatact aagcgacaaa aatgatttaa atacatctca agaagttcta atcactgtca
     9361 atgtaaaata atatgtcaac ttattcaatc attctatttt caatttaagt gtataatcaa
     9421 aataaaggtt gttagtaaca gaacgctaat attagtgctt tgttactagc aacctttaaa
     9481 ttttatagaa atagattatc taaaaaattc catttcttct gttagtagcc catattccta
     9541 ttaaaattat cagtgaatat attatataca aaactattct tatatattgg acatctaatc
     9601 caaaaaattc aatgttactc tgtgaggtat gcaacatcac aaggactgca gatataccaa
     9661 taagcaaagg attgattact atttgcttat cagttcttaa aatcattatt ttacccatcc
     9721 aaattattga taatataaaa aataaaattt taagaccaaa ttctaatgta tctaccaaag
     9781 cgaaaacatc ccttcattta taataaattt ttcagctcaa caaaatataa aaccctacaa
     9841 acaacttttg aatccataat aagataactt gattaagcac tatgaaataa gtactataaa
     9901 acttcatcta cattaatata tactacacta tacttcaaat ttcttaaaca taaattaatg
     9961 aaattaacaa aattttattc agaaacatat aaagatatat tttactattt aaatgcatat
    10021 cattatatat acagttttag gaggtgaggt atatggaatc taaatttttt aattggtaca
    10081 ctcaatcctt agggggaact tttggcatta tatcatgtat atgcgcatat ttaaatggat
    10141 acatgtttgt ttatacaaat agtgctagca aatattttga aagtatagga tttctgggaa
    10201 taatttcaag ttatacacta cttccacttt gtatgcttac tttgattttt ggagttatat
    10261 attcttatac tccagaaaca tccgacaaaa gtatttttaa tatacctttt tttactttaa
    10321 ataaaagatt aatacttatt acagtagttt ttggttttat aggtgctaga gtttacttta
    10381 taattcctgc aattctaata acttttaacc tatatagtaa tgttttgata gctaaattaa
    10441 agtacatcct atgtaaaaat aaagaaaatg atactaattt gaaagaaagc gaagtctaaa
    10501 ttgcataata ttggtatttg tcttaaaaat tcccgaggta ttaatttgta ttctcttgta
    10561 ttttgcaaaa aatattgaat tttattcatt ttatacttat aatttatatt aataatggaa
    10621 a
//
LOCUS       DACTGW010000136        10555 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.136, whole genome shotgun sequence.
ACCESSION   DACTGW010000136 DACTGW010000000
VERSION     DACTGW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10555
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.136"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            68..1048
                     /locus_tag="I9573_002545"
     CDS             68..1048
                     /locus_tag="I9573_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAT4797155.1"
                     /translation="MNLKLIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLL
                     LYYNKFIDPENFASFMMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVL
                     KLLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIV
                     PTSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKL
                     GVIILGMCWSLWHLPLIFTLYTPEAPILGVILRSIHIVGISIFLGYLYIKTKNIWFCA
                     IIHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1147..2109)
                     /locus_tag="I9573_002546"
     CDS             complement(1147..2109)
                     /locus_tag="I9573_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAT4797156.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIITVMAAFYIFEYYLSTILMEI
                     IRKILIATGDINLKTNYFSEYANWLNDVGLQVVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(2429..3379)
                     /gene="hpdA"
                     /locus_tag="I9573_002547"
     CDS             complement(2429..3379)
                     /gene="hpdA"
                     /locus_tag="I9573_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HAT4797157.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFKDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSAENISDIISFMHKNNLIEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDIDEEHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3372..3629)
                     /gene="hpdC"
                     /locus_tag="I9573_002548"
     CDS             complement(3372..3629)
                     /gene="hpdC"
                     /locus_tag="I9573_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HAT4797158.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDSACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(3633..6341)
                     /gene="hpdB"
                     /locus_tag="I9573_002549"
     CDS             complement(3633..6341)
                     /gene="hpdB"
                     /locus_tag="I9573_002549"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HAT4797159.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            complement(6638..7654)
                     /gene="tsaD"
                     /locus_tag="I9573_002550"
     CDS             complement(6638..7654)
                     /gene="tsaD"
                     /locus_tag="I9573_002550"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAT4797160.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSNKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(7660..8136)
                     /gene="rimI"
                     /locus_tag="I9573_002551"
     CDS             complement(7660..8136)
                     /gene="rimI"
                     /locus_tag="I9573_002551"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAT4797161.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKCIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(8126..8842)
                     /gene="tsaB"
                     /locus_tag="I9573_002552"
     CDS             complement(8126..8842)
                     /gene="tsaB"
                     /locus_tag="I9573_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAT4797162.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(8842..9294)
                     /gene="tsaE"
                     /locus_tag="I9573_002553"
     CDS             complement(8842..9294)
                     /gene="tsaE"
                     /locus_tag="I9573_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAT4797163.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(9433..10473)
                     /locus_tag="I9573_002554"
     CDS             complement(9433..10473)
                     /locus_tag="I9573_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HAT4797164.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWEDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
BASE COUNT         3407 a         1907 c         1086 g         4155 t
ORIGIN      
        1 atttataata ttcaataaaa tattttaatt tagttaagaa tctatattgt aaaggagtat
       61 taaattaatg aacttaaaat taatatggat aaatattcta aacccagtat ttattaaaaa
      121 tgaaataaaa agaaaaaaaa tacaaattat tatctttttc tttataaact ttggtttaac
      181 ttattcatta ggtttactgt tatattataa taaatttatt gatccagaaa actttgcatc
      241 attcatgatg atattacctc tatcttcagt tgctattgca aagttttata ctgaaggatt
      301 aaataatgat atgtatgaat tttattcact aatcatatta ttttttctta tttattcatt
      361 attaattgtt cttaagttat taaatctaat aaataataat gaatttataa ttataaacct
      421 aatattaact tttataagta gcttatcaat tataatatat tcatcaaata aaaaaaattt
      481 atatcctgta aaaaatatta aactaggttc tttattaatt atttacttta tgatttctgt
      541 aattgttcca acatcattta tactaatact acaaggggct caacctaatt atagtggtat
      601 cataaaattt atatttactc caataacatc attaactact atttatattt tttttagtga
      661 agaatatgcc tggagaggtt ttttacagaa tatttttttt gataaatttg gtaaaaaatt
      721 aggagtcata atattaggta tgtgttggag cttgtggcac ttaccactaa tatttacatt
      781 atatacacct gaagcgccaa tattaggagt aatattacgt tcaatacata tagttggaat
      841 ttcaatattt ttaggatatc tttatataaa aactaagaat atctggtttt gtgctataat
      901 tcatgtttta aataatagtg cttttcttat cacaaattct aaagaatcaa ctatcactta
      961 ccatgatata ctaatagtat ctatggttgt tttaattttt tatgttccat ttctatttac
     1021 aaaagaatat aaaaataact tggagtaaat agaatatcca agttattttt atatttttgt
     1081 aaatttccaa caatcctaca gaatacttta agtttagaag atattattaa aatttattat
     1141 aatcaactaa ttcttgatat tagtcacatt tactttatca ctaacaaaat catacttaga
     1201 atctctatag taataccata caaatataaa gctaagtaga ttaacaaaag tagtcaaaat
     1261 tccaccttca ataccaaaca tccctccatt taatagatta ccatgagtga tttgtgtagt
     1321 tataatccct ggaacctgaa gtccacttac aggcatacca tatataatcc cctgaaatac
     1381 attccatgta aaatgataac ctatacacat ccatatattc gacgacttta taaacatata
     1441 tgaaaaaaga atcccaataa ttataatatt aattacagac aaaaaagtaa catcaggatt
     1501 ccatatatga atcaacccaa aaataacaga tggaacaatc attataaaat aaatattttt
     1561 acatcttcgt aaaactgcca taaaaaaacc acgatttaaa atttcttctg caaaagcaac
     1621 taaaataaag acaagtatcc aagaaactgt taaaggtgaa atttttggta cccatgaagt
     1681 aacatgacca ccaccaattg ttattgttat aataaagatt actgtacaat taaatattcc
     1741 taataacatt cctacatatc cttctttttt cttaattcta taagaatgta atcccatttc
     1801 actaatagga tttttcataa taaatctcca tacaattata ggaataataa tcataagtga
     1861 atctgttaga atttgaaata caacctgtaa cccaacgtca tttaaccaat tagcatattc
     1921 cgaaaaatag tttgttttta aattaatgtc acctgtagca atcagaattt ttcttattat
     1981 ttccataagt atagtgctta aataatactc aaatatgtaa aaggcagcca taacagttat
     2041 aataatccac cctgaacgta cttttttgtc tttattctta ataatcaagt tactccatga
     2101 tttgttcata tagatgtctt cctcctaact attattttta gttgtttcta aaatatattc
     2161 tattaaattt cttcatagta gtattttacc acaaaacttc tgcttcaata aagtaaacgt
     2221 aatatattct ctaactgtaa tttaaactat aaaaaatata taaagtataa atttaaagta
     2281 atatttatgg acataaagca atatgaacaa tatataaaac atataaaact ttacttaatt
     2341 atttctacat aaaaaccaca taaaaaagga tgtaactcta gcttttagtg taacatcctt
     2401 ttttaattaa aaaaacttgt atttgatttt aaaaagctgt ctcatgacca acatagcaag
     2461 ctataccatt atccaaaaat atatcttgta attcttcaag atgctcttca tcaatatctc
     2521 ccttatcaga atactcatac tccttaccta attgaatcca ctttgattct cctagcctat
     2581 gaaatggtaa taagttaatc tctattaaat tatttttgtg cataaaagat ataatatcac
     2641 ttatattttc agcactatca ttaaacccag agataactgg tactctaagt actaaccttc
     2701 cattccagtc agaatttgca aggtttgata tattttctaa tattaaatca ttatatacac
     2761 ctgtttgttc tttgtgtttt tctctatcca tatgctttat atcaataaat gcaaaatcaa
     2821 tatctttaaa tattttatta aaaacttcat ttgatacaca tgcacttgtt tcaatggcag
     2881 tgtgtatatt tacctcatga cactttaata atacttcatg taaaaattca tgctgaagta
     2941 gtggctcacc tccactaaat gttactcctc cattacttct ccaattgtta gaatcacgtt
     3001 taattacctg cacaagttca tctacagtat aaggttttgc acataattta aacgcattat
     3061 aataacatga gttgacgcat tcaaaacttt cacagtcctt acagatgttc caatctataa
     3121 ctggcttatt atcaagatta aagctcaatg caccattttt acacttacca tgacatacag
     3181 tacatccatt ttcatattga caagataatt cactaaacat catatgtggt cttacagtcc
     3241 aactttctgg atttgcacac catttacaac tcagtggaca tccatttaaa aatacagttg
     3301 ttctacaacc tggaccatca tgaactgaaa agctttgtac atcgaaaatc atgccttcta
     3361 attgcttttg actactcatt aaacacatgt ccttcacaag taattgcatt caatgttgaa
     3421 tatacccagt cttctttctc taggccaaca catttgccta taccttcatc attagcttca
     3481 acaaaatttt tacaattttt acattttggc attactttta tctctggaca tgctgaatca
     3541 tctaaattaa tcatttgttt tgataatcta caaattcctt tggttgcatc tactgcacaa
     3601 aaattcatac aatcactatg ctttctcata ttttacaccc cttcatactc tgttctagca
     3661 attacttcat cttgtattgg tttacctaat tcacaccaat attgagtaaa cccagcaaca
     3721 cgtaccatta attgacgata attgtcagga tttttttgtg catcttttaa tgtttctgaa
     3781 tctacaacat tatattgaat atggaaccca ccttttctta aataacttct tgttaaatct
     3841 aataattttt tagttccttg ttgtcctttt atagtagttg gatgaagttt taaattcatc
     3901 tgagaatttt gaactactgc ttggtcccaa atagttgcag attcaaataa tgcatatact
     3961 ccatttttat ctgttcctgc atatgcagag actgaaccat ctgaatatgt tgttccactt
     4021 agtctaccat ctggagtagc caaggtagca gctccctgtg gtccatgtgt agaaacagat
     4081 atttggcaag ggtacatctt cttagcatat aaagattgtg cttcttcaca tacttttgat
     4141 aaccatacct catagttttt aagaatatta tctgcataca aatcattatt tccatattta
     4201 ggtgcatcaa gacaggcctt gtaaatagca tcatatttac cagtcttatc tacttttact
     4261 tggtctgcca tagaataatt tcctacttct aatgcatctt taaatccaaa attatttaat
     4321 attgcatcct tcatttcctc tattgtaaat gctttgtcat catatacaag tttcttaagt
     4381 gctgcaaatg aattgaccat agtaacagta ccacaagttt ctacgtttaa tgttgcatta
     4441 taacgatatc ccatatttcc tatatgttga cctttgtcta aacagtctgg ctttagtagt
     4501 gaatttatta tagaagtatc aaattttctc catatatcta actcaatgtt atttgctctt
     4561 tctaaaacat ttattgcttg tttataataa tctttaaata cttctatgac ttcttcataa
     4621 gtatctaatg gcttattatg aggctcaaat acttgtatat tcatacgatg gtctttacca
     4681 ttcataagaa caagttctaa aattttggga ttagctataa agtgaactcc agagccagca
     4741 gattgacctg ctccacctgc aatagagtac gtttttccgt ttaaagttaa ctgtttccaa
     4801 caccctggag acgtttccaa acatccacct aatgcaaatg ctcttgcttc ttcaacagtc
     4861 ataccttcat cagcaaattg tttcatcata aatgttgttc catttgagtt gtttacccag
     4921 gctggataac cagaaccaag ctttgtacat tcagcagctt tcattaagaa atcttctgga
     4981 agcttttcat catatagcat agttaaacta ggttgaggag ttctacatct catacttgct
     5041 tctaataata actcttctag ttcattagct cctgtagaac catcttcttt taatccacct
     5101 atagataaag tattaaatgt attacctgat agtactcctc catttacgcc cgctgaagca
     5161 aaacagtcaa tacaagtgat ttttattcta taaagctcta ataattcgat aacctcttct
     5221 tttgtaatta gtcctttttc tatatcttgt tcataccaag gatataaaat ttgaccaaaa
     5281 cgtcctatag acataccaga tatagcatcc tcatttactg atgctatatg aattgtataa
     5341 gtcaactgaa gagcttccct aaatgtacga ggttgtttat gtgcaatatg ttctaatatc
     5401 tccactaaat ctgagtattc ttttttagct tctaaatctg tctctatact ttctaaatat
     5461 tttgcatgtt ttgcataatt taaaatccaa gtttgtaatc cttcaataac ttgaataaca
     5521 gcctcataaa aatatagacg gtccatacca ataagcccat ctccagatgc atttcctgca
     5581 actgcctttt tacattcttt tgccatttct ataattccat ctaatccata atttaaagga
     5641 taaaaataat ttataacttc tctaccttga ggaagtgtat accctgaatc aaacatgcat
     5701 ataagtgtac tcattaggtt ttcttttaaa tcataatctg gcatcatttt ttcatagtga
     5761 aaacctaaat cttcaacaga ctttccaacc cattcctttg ccatttttac aagtacaggt
     5821 acttcttctt ttctcattcc gaatttacca gctattgaaa cgacatttcc aaaactttca
     5881 gtaacatttc ctccaccaga cccaaatttt gtaagttcat cagctgtgtt actggctaat
     5941 ttctttgcct cttctctcat ttgttctcct tgagcaacaa agaaactttc acttacccat
     6001 ggcattggaa aagaacctct ataatggcgt gtcttttgca ttactatttt ctcaccaggt
     6061 attatatttg gtgtcatatg tgaaaaagca gctttaagag ctttagctct tctaactact
     6121 ggtatatcac catctgattt tctatattct ctattatacc aatatggaaa ttccatatca
     6181 gcagtagata atgtattata atatacatct aataagtctt ctgctctttt acttgctttt
     6241 ttttcattaa attcacttaa attttcttta ttttgaaaat ttgatttaat attttttgct
     6301 tccaaaatac ttcttatttt gtcttcttta ctttgactca tttcttcccc tccttaatct
     6361 ttctataatt agtataaaac tttttaagaa tgaaaaaaaa gggtatatta cccacttttt
     6421 aaaataatct ttctatagaa aaaattaaac ttgaagcaaa tctaagccaa agacaatctg
     6481 tatatcctaa aatttgttgc aattcattgg aatataaaga ttataaatag ttttacttca
     6541 agttttaata tatactatat ttatttttat tatatttaat ttcacttcta cttacttttt
     6601 tgaaacaata cttttatatt tacaaattca tctaactcta ctgatttatc tttaaatttg
     6661 gaactgcatt taaactcata tcatgcgttt tcccatttat aaaattataa tatcctgcac
     6721 accctatcat agctgcatta tctgtgcata aaattagagg aggatactta acagtaatac
     6781 cattgtcttt agcaagctct gttattttag ctctaagccc cgaattagaa gcaaccccac
     6841 cagaaagagt tatgatattg tatcctttgt cttttactgc ttttagtgct ttattagata
     6901 aaacctctac tacagcctct tggaaagatg ctgctacatc ttcaactact atttcttcat
     6961 ttttcattct ttttccattt agataattta atacagaaga ttttaatcca ctaaaactaa
     7021 aatcatagct atcttcttct aaataggctc ttggaaattc tatagcatgt ttatttccat
     7081 tcttggccag attatcaata ataggacctc ctggatagcc taaattcata gctcttgaaa
     7141 tcttatcaaa tgcttctcca gatgcatcat ctcttgtttt tcctaaaatt tcatacttgc
     7201 cataatcttt tacttcaacc aagtgagtat gacctccaga tactattaat gttataaaag
     7261 gaggttttaa atccttatgc tctatatagt ttgcacttag atgcccttct atatgattta
     7321 ctcctacaag tggaatattc aaagtatatg caagtgcttt agcataagat agaccaacca
     7381 ataaagcacc tactagccca ggtccatatg taactgcgat atggtctata tcattaaaac
     7441 ctatatttgc tttgtctaaa gcttcttgaa caactatatc aatattttcc acatgttttc
     7501 ttgaggccac ttctggaact acaccaccaa attttttatg tgtctcgatt tgagtagaga
     7561 ttatgtttga aagtacttct ctaccatttt ttaaaactga tgcagctgtt tcgtcacaac
     7621 tactctctat agctaatgtt attatatcac tcataaactc tacacctctt ttaattggtt
     7681 ccacattata attgcatctt ctttattatc actgtagtat tcttttctta ttccacccat
     7741 tttaaatcca tattttctat ataaattttg tgctatttta tttgatgctc taacttctaa
     7801 tgtcatagat gcaatattat tatctttaca taaatcaact atgcatttta tcaacttgtc
     7861 ccctattttt ttccctctat agtcactatg aacagccaca ttatttatat gcccttcatc
     7921 aagtataagc caaattccca catatccgac aatttttcca tttaacttag ctactatata
     7981 ccttgctact tcattagaca actcttttct aaaagactct tttgaccaat aatcttcaaa
     8041 acaatttttt tctacttcaa aaacttcatc tatatcttct gtagtcattt cttctatttt
     8101 tatatcatct actattagtt ttgtattatc ttccattatt tagcctcttc attttttcat
     8161 catattgtac ttcagcttga gattttctga tatacatagg atttatcgta taacaagtat
     8221 gtacatctat atttttatta tatttattta atgctaaaga gcataagctt cctgcgttag
     8281 atacattatt agctggtgaa ggtattttta tattttttat attctctaac ttgtctttat
     8341 atttgtatac agcttctcca actattataa attcttcatt agttgataca atttcatcta
     8401 ctaaagcatc aaactctaca acatcaacac catctaattc tatcatctct ccattttcaa
     8461 acttgtattt agctgaataa acttgatttt tctgcgcatc taatatacaa catatctttc
     8521 tatcacagaa atttatgtta tttgctagag attccaaaga atttactgct attattggta
     8581 tattatttac atgggccata gcttttactg tagccatacc tattctaagt cctgtaaacg
     8641 aacctggacc tatacataca gctaataaat ccatatcttt tatacttaaa tcactcatac
     8701 tcaacatatt ttctatcatt ggcataagct tttgtgaatg agtttttttg ttgtttactg
     8761 taaactcaca tattaagtta tcatcttcaa caactgctac acttgctgcc atagaagacg
     8821 tatccatacc tagtattttc attatttaat caactcctca actatctctt tataaaactc
     8881 tccttttgga gttaatatca tttctcttcc ttcatcttta tatctcaatt ctatattcaa
     8941 atattctttt ggaagtatat cttctattag attagcccat tctattatac agattccatt
     9001 tgaatttaca tattcatcat aaccaatatc atacatttca tcactactac ctattctata
     9061 aacatcaaaa tgatacaaag gtatttttcc ttcatattca tttattatag taaaagtagg
     9121 acttgttata taatcttcta tccctaatga atctgccaag ctttgtgtca ttgtagtttt
     9181 accagctcct aaatctccta ctaaacagat aacactgcct tcttttaaaa gtttacctaa
     9241 tttatatcct atttctctag ttttattttc attttctaaa tatatttttg ccatcaattc
     9301 atctccattt tgatataatc gtcatgtgaa tttatatcac tgcttaatta attatagtat
     9361 aagcctagat tattataaag tacttgtctt tatctttttt tatctttcac tattgatggc
     9421 tgtatctaca ccttatatac aatcatcaat agcaattttt acatactctt ttgctttatg
     9481 gttttgcttc aatgcttctt tacatattct ctcagattgc tcttttacat acttaagaga
     9541 cttatagttc tcttttactg cttcaataca cattttttct gtttgatttt ttatatacat
     9601 aagcattttt ccattttgtc ttacaatctc tatacatata tcttctgtat acttatctat
     9661 gtaacttagt acatctttaa tattatattt tttatttaaa tatcttatag catttataca
     9721 tatttcttct gtcttattgt tgacaaattg tagacttctt ccatcttgtt tgacagcttc
     9781 tatacatatt ttatcagttt gtttatttac atattgtaat gccataccat tttgtcttac
     9841 ggattctata cacatttttt ccgtttgatt ttttacatat tttaatgctt tataattttg
     9901 cttaatggcc tctatacata attcttcact ctgttctcta acgtatgcaa gacacctgcc
     9961 atctttttta attgcttcta aacatatcct tcttgtttga gtctctaaag cctttagtat
    10021 acttacaaca tctatattgg aagaatgact tattgctttt ataataagcc cttctgtttt
    10081 atttcgtaca tacttaagtg cattacaatc tttttttata gcttcaagac acaagtattc
    10141 tgtttgttct tttacatatt caagtgcttt ataattatac tttattgcct ctaaacataa
    10201 ttcttcggtt tgatttttta catactctaa tgccttgtaa ttctgcctta ttgctaaaat
    10261 acacatacca tcagtttgat tttctatatt ttttaattca cgataatcgt attttaccct
    10321 ttccatattt tcaatatatt cattagtgat atactttaaa gaatctccta attttgtatc
    10381 ataaatacca atatgaatat cttcccaatt actattaaaa taataccctt tatcatctaa
    10441 aaataatttt tttatttttt tagataaatg catgaatata cacccctatc attattaaat
    10501 tagttattat taaaagtttg attattagtc tttatagttt tttaataata acttt
//
LOCUS       DACTGW010000137        10435 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.137, whole genome shotgun sequence.
ACCESSION   DACTGW010000137 DACTGW010000000
VERSION     DACTGW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.137"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            309..974
                     /locus_tag="I9573_002555"
     CDS             309..974
                     /locus_tag="I9573_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAT4797165.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            996..1928
                     /locus_tag="I9573_002556"
     CDS             996..1928
                     /locus_tag="I9573_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAT4797166.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            1949..2590
                     /gene="pcp"
                     /locus_tag="I9573_002557"
     CDS             1949..2590
                     /gene="pcp"
                     /locus_tag="I9573_002557"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAT4797167.1"
                     /translation="MKILLTGFDPFGGELINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIK
                     GGFIHIPYTTSQVINKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(2709..3041)
                     /locus_tag="I9573_002558"
     CDS             complement(2709..3041)
                     /locus_tag="I9573_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4797168.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            3307..3927
                     /locus_tag="I9573_002559"
     CDS             3307..3927
                     /locus_tag="I9573_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT4797169.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(4252..5076)
                     /locus_tag="I9573_002560"
     CDS             complement(4252..5076)
                     /locus_tag="I9573_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HAT4797170.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     TLFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(5087..6490)
                     /locus_tag="I9573_002561"
     CDS             complement(5087..6490)
                     /locus_tag="I9573_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4797171.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(6494..7219)
                     /locus_tag="I9573_002562"
     CDS             complement(6494..7219)
                     /locus_tag="I9573_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4797172.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDVQNESKNLNKNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(7331..8104)
                     /locus_tag="I9573_002563"
     CDS             complement(7331..8104)
                     /locus_tag="I9573_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797173.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFLGFLLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVRYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(8110..8997)
                     /locus_tag="I9573_002564"
     CDS             complement(8110..8997)
                     /locus_tag="I9573_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797174.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDANYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVFDEP
                     TAGIDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSGDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(9011..10033)
                     /locus_tag="I9573_002565"
     CDS             complement(9011..10033)
                     /locus_tag="I9573_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797175.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETERYYID
                     KEKGCIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
BASE COUNT         3726 a         1583 c         1182 g         3944 t
ORIGIN      
        1 acttgtctct atgtttgaca cattctcttt ttataatata atatctaatt taaaacctat
       61 ttcaaatatt gacatatact taaaccatag acttacaaaa tcaattcata ttagcttaaa
      121 atataatctt tgttactcaa attcctatac acaaccatta tttactttga tactttaaaa
      181 catcttccct attttttatt aaattttata ataatagtcc tattaacgca aatactataa
      241 aaagagttta actttatcca ttaaataaat ttaaattaaa acatatagaa atggaaggag
      301 aacaatttat gataaaacta atcggtatat tgatagttgt aattggattt atacttaaaa
      361 ttgacacatt atttactgta ttattagcag gagtggcaac aggaattgtt gcaggattag
      421 attttaatca aatttttaca attttaggag attcttttgt ctcaaacaga ggtgtatcat
      481 tattcatatt aacactgcca gtaatcggtg tattagaaag atatggttta aaacaaagag
      541 ctgtttcttt aatagaaaaa ttaaaaagac ttacaacagg aaaagtactt actatataca
      601 tgatagctag acaaatagct ggtgctctat caataagaat gagtggacat cctcaatttg
      661 taagaccact tgttaatcct atggcacaag ctgctggttt aagtaatagt gatgaactaa
      721 aagaatcgga tgaagaagcc ataaaggcat tatctgctgc atcagaaaac tatggaaatt
      781 tttatggtca aaacttattt gcaggtagta gtggagtatt acttatagca tctactctaa
      841 ctcaatttgg atacaatgta actggactta atattgtaaa ggctagtact attatggcag
      901 taatagcact tgtagtggct acaatccagt ttaccttata tgataaaaaa ttaaacaaaa
      961 gtcataaaaa atagttttat ttaggaggaa taaaaatgca aaattttata aacatatcat
     1021 tggaaatctt ttacgcacta atgggatttt taatgattgt tatagcatat aagtctttta
     1081 ctaccataaa taacaataag aaatatggta catcattatt ttggatactt atatcactgc
     1141 cattcatatt tggtagattg attccagcca atataatagg aataatatta atattatcta
     1201 gtttattaac actttctaaa caagttgtat ttgcaaagta tgaagaacca gatgaaaatt
     1261 ttggaaaaga acaagcagac aaacttaaaa ataaaatatt tataccatca ttaatattag
     1321 catttgctgc agtagtagta gctatgagcc tttctaattt tgataattct tctcaatttg
     1381 caataggtgt gggttcaata attgcattaa tatcagccct tataataact aaagcaaaac
     1441 ctgctacatc agttcaagat ggctcaagat tacttcaaca aatgggacct gctagtatgt
     1501 taccacaact tttagttgca ttaggagctt tatttactca ggctggagtc ggcgaagtta
     1561 tttcaaccat gatatctggt gtagtccctg ctgatagcag attatttggt gttatagctt
     1621 atgtattagg tatggttata tttaccatga taatgggaaa tgcatttgcc gcattttcag
     1681 tcataacggc aggaattggt attccttttg tattgtctca aggtggaaat cctgctataa
     1741 ttggagcatt agcattaaca gcaggatatt gtggaactct acttactcca atggctgcta
     1801 attttaatat agttccagca gccttgctag aatgtaaaaa tgattatatt gttataaaat
     1861 atcaagctcc agttgctttg gtattaatta tagcccacat attagtaatg tactttttag
     1921 gattttaata taattaggag gtaaaataat gaaaattttg ttaacaggat ttgacccatt
     1981 tgggggagaa ctaataaacc cagctcaaga agcagttgaa agggtcaata ataatataaa
     2041 cggtgcagaa atcataaaga taactatacc aacagttatg acaaaatctg ttgaagctat
     2101 agataaagct attcaagagc acaatccaga tatagtaata tcagttggac aagctggagg
     2161 tagatttgac attacacctg agagagttgc tataaatata gatgatttta gaataaaaga
     2221 taatgaaggt aaccaggtta tagatactat tataaaagaa gatggtgaac ctgcatattt
     2281 ttcaaaatta cctgtaaaag ccatggtaaa acatatgaat gaaaataaaa ttccagctag
     2341 tgtttcaaac actgcaggaa cattcgtttg taatcatgtg atgtatggaa tcctttatat
     2401 gatagacaag aaatatccta atataaaggg tggatttata cacatcccat atacaacttc
     2461 acaggtaata aataaaaaaa atacaccatt catgtcttta gaagaaatag taaaaggttt
     2521 agaattagct atagaagctt gtattatata caaagaagat gtaaaagaaa ttggtggtga
     2581 aatttcataa taaatttttt agtattttat aaattaaaaa tactaactat taatttaaat
     2641 atagactgtg agtagattat tcaaacaatt ttactcacag tctttttatg gcatctacag
     2701 atacaaaatt aaggtctatt cctctctccc cactcacaca ttgcatctaa aattggaatc
     2761 agagattttc ccctttctga taaactatat tctaccttag gaggtatttg aggatattca
     2821 gtacgaataa tcaagttatc tgcttctaat tcttttaatg ataaactcaa agttttatag
     2881 gaaatagtat ttatataacg ttttaatgca ttatatctga caacttcaaa ttctacaaga
     2941 cagtaaagaa ttatcatttt atatttacca ctaatcagtg aaagtgtata actaaatcct
     3001 gtttcactta aatctgtatt tgatatacag tctatactca tatattacac tctccttttg
     3061 gttagtattg aacaaaaatg tgcgtacttg tttttagaat aatttataat aaaattatag
     3121 tgtaagaata attgaaagtc aattttataa taggaggtgt atttactaaa aaaagagtta
     3181 ttttaaatag gattctaaga ttagacattt ctatataaat tgataatatt catgaaaatc
     3241 ctattttata aaaaataata tctcttagaa aatctattaa ataaaattca ttggaggaaa
     3301 tttataatga aagttttatt gataaacggt agcccaaacc aatatggatg tacttataca
     3361 gcactaaatg agattactaa agtattgagt aaacacgata ttaaaacaga aatactgtat
     3421 ttaggtaaag aagcaatccc aggatgtatt agttgcgcat cctgttttga aactggaaaa
     3481 tgtattcgaa atgataaagt taatgaattg attgaagatc tcgataatat tgatggtatt
     3541 attattggtt ctcctgttta cttttctagt gcaactggtc aattaacttc ttttcttgac
     3601 cgtctatttt tcatagctgg aagtcgcatg gctacaaaat taggtgcttc agttgtgtca
     3661 tgtagacgtg gtggtgcaag tgcaacattt gaccaactaa ataaatattt ttcaatcagc
     3721 aatatgccta ttgtatcatc acagtattgg aatcaggtac atggctttac tccagaggat
     3781 gttatgaaag atgaagaagg attacagact atgcgaactc taggagaaaa catggcatgg
     3841 cttcttaagt gtatatcagc aggtaaaaaa gctggaatta aagaaccaca atatgaagaa
     3901 acaattatga ctaattttat aaaataaata aaaaatgcac taatattaat taaaacaaaa
     3961 cactaattta taaataaaat aagtataaat ataggataaa aaacactaaa acagaggtat
     4021 tgaattttat atcaaacctc tattttatta ttacattaat aaccaaacca tatataacat
     4081 catatatttc taaataaatt ataatattat attctaatgt tattgaaatt acaataactg
     4141 aatttctgct cataaattta aatattctac aaatctcatc taaataacaa ttttaataga
     4201 cctaaaacaa tcctcatatt gttcattttg tattaaaaat atcacttaca atcaaaaacc
     4261 atttattaag tatattccta agatatacaa tatatataca tggattggat agtataaata
     4321 aaataaatac ttcatactct tccctttttc tccattgtaa agtaacatta aaggtagaga
     4381 agcaatcatc atccattggt aattttgtaa aatagaattt tttaaactat aatctccatt
     4441 tgacattgtt gtaaatataa atatacacat caaaacataa caaatactca aactgaactt
     4501 tttacctcta cacaaataaa aaataatccc aagtataatc caaattggtc caccttctac
     4561 taaaaatggt actggtacca aaatcataaa taaagtaata actctgttag tgactttaaa
     4621 aagtaaagct actgtaaata ttcctaaaat tataggaatg attatcatca ataatcctat
     4681 aataaaatat ttaatttctt tctcttttct aaatttcttt atgaactcta ttgcttgtaa
     4741 ataaatcgct attaaaaata atgttgaaaa aatgttattt ataattatag ctccttctgg
     4801 atgtgaaaat acattattaa ttatttgatt tcctatagcc ataataacag accaaatata
     4861 caatctactt atatattttt ttctgttact agtgtgataa aatccttcaa cagccatgaa
     4921 tataaacagt ggtgctgata ttctgcctac ccaatgaaac caaattggta tttgcattgt
     4981 ttgtggcata aatgctgcta tatggtcaaa tgtcataaat attaatgcta aaatcttaat
     5041 agtaaaccca cttatacctt ttttaaaaaa atccatatca tatctcctaa ttttgtttta
     5101 tctctataat aatttttgtt cctattccaa tttcactact tacataaatt ttacctttat
     5161 gaatctctat tatttcttta gaaatagcca taccaagacc tgaaccttta tgcatctcac
     5221 ctgtattagt acctctataa tattttttaa aaatatgttt caaatcactt ttgcttattc
     5281 caattccatt atcttttatt ataatttcta tattatcttt ttttacaatt tctacactta
     5341 tcaaagttcc ttcgctatta tgaactattg aattaaatat aagattggtt atagcccttc
     5401 tgaataagat tgaatctaca tatacttcaa ttaaatcttc attactttca aattcaatat
     5461 tcctattttt atatctatta tcatttaaaa tatcaattat tatatctctg actaaactga
     5521 ctaaatttat ttttttcttg tccagaacta tagtattatt ttttaatcga gtagataaat
     5581 ttaaatcatc aactaaatct tttatatact tagacttatt atatataatt tctgtatact
     5641 ctcgtatttc atcttcttca aactcataat ctttgtcatt aattatctct gcataccctt
     5701 gtatagatgc tagaggtgtc tttatatcat gtgttatatt tgccagccat tcctctctca
     5761 actcttcatt ttcttttctc tcaacttcat ttactcttaa agtatcagct aacatattta
     5821 tatttttaaa aacttcttca tatatacctc tgtccttaag atataaacta taatttccat
     5881 tagccatagt ctcaatactc catagtacgc ttgaaattgg ttttgtaagt tttctactaa
     5941 ataaatatcc tacacctaaa gctaccacgc tatcaattac tagtgttata atgataactt
     6001 tatttaaagt ttttttaatg ttattttgag aataggttaa aattattctt gatatatctc
     6061 tactaggtat acctactatt atgtttagat gttttttgtt taaatatttt tctagtacaa
     6121 aattaacata ctttgtttct atatacttgt aattattcac catttgaaag ggtgtatatt
     6181 tttttggagt atcctttggc acatttactc cataaacttc ctctcctaaa tcatttaaaa
     6241 cctgtatcca ggaatttgat ttttctaatt tttcagctcc aatcttagat agtttaaatt
     6301 ctccatcttt ttcatataaa tctttttcaa aagaacgtgc aaattcttca ggattatttc
     6361 cagaatctac aactttaaaa aagctattag ttgaaatcac atacaatata gatataatat
     6421 ttataattac tactgatata gctacaaaca caattgtaaa tataaaattt ctagtaatct
     6481 tccacttcat attctaatct tcctttactg ctaatttata tccaagacct tttattgtca
     6541 gaatatattc aggctttgaa ggattatcct ctaatttttc cctaattttt cttatatgaa
     6601 ccattatagt attgtcaaat ccaacataat cttctcccca cactttatcc catagttttt
     6661 ctttacttat aatttgattt tgattatgtg caaataaaga taggattttt agttcctttg
     6721 cagacagtcc tatagatttt ccatctttta taagttctgc cctactttca tttaatatat
     6781 atgggccaaa tgatataatt ttttcttcat tcttatttag atttttactt tcgttttgaa
     6841 catcactaaa ataacttaat cttctcaaat gcgctttaac tctaaaagcc acttccttag
     6901 ggctaaaagg ttttgtaaca taatcatctc ctccaatcgc tagtccaagt aacttatcta
     6961 attcttcggt tttagctgat aaaaatatta ttggagaatt ggaggttttt cttatttctt
     7021 tacaaatatc ataaccttcg ccatctggta acataatatc caatacaatc aaatcaggat
     7081 taatactttt aaataattct aatccttctt ttttagtttt tgcagtatat atattattaa
     7141 ggccctcttt ttttaggaca gtttctaaaa gcttcaatat ttctacttca tcatctatta
     7201 ttaaaacacg attttccata aacaacctcc tgtaataatt tacatatata ttagcatata
     7261 ttttatctaa aattcattta tcttattttt aattaaacta aaatataaat aaaattacta
     7321 ttatttttag ttaaaacctt ttgcttgatg taaaattttc atatgttatt ttagttaaca
     7381 ctatcattaa tataattgag attactatag aaaaaactat caaaatactt ggtacattat
     7441 acctaaccat ttgttggctc atcataatcc aatttggagt cactgacata aattttatag
     7501 ctttatctgg tataaaacct acactaaagt atggaattgc cattatcaat ccacctagta
     7561 taaatggaat tacagccgat ttaaatatag atgaaagtgt taagcctatt tgtgcaaata
     7621 taaaagaagc aaaaatagta gtcaatataa caattatata catttgtatt actgtaagat
     7681 ttaaattact atttccaaag taccataagt tatgaatcgg ttcatttaaa tttttaaaat
     7741 caaaaaaact aaaacctcta attcctacta acaatataaa agaaatatta ataatagata
     7801 aaattaatcc attagctact agttttgata atactactga tttttttccc tttttagttg
     7861 tatttattaa acctgtcata ttagaatcat attctttaat gtaatttcca gaagttatta
     7921 taaacattat caaacctatt agaggtattg agccttctat cttgtaaaat atatctgcat
     7981 aagtatctaa aacatcaaac tttctaatca gaagaaatcc caaaaatatt aaaaacagcc
     8041 aaattatcca aacactcttt gattttaata tttttatcat ttcagcttta actaaataca
     8101 tcatttctac tacacctccc ttgcatcttc caaatcaaaa taaaacatgt atacatcttc
     8161 gaattttgct tgaactggtt gtatattatc cccacttgga atatcctctg aaacaattct
     8221 aagtactact gaatcaatat cactttgaat acttacaacc ttatatttag attgtatatc
     8281 atcaacttca cattcatttt taacttgaac attatatact ttattttcca tgtgacttaa
     8341 tatgtctttg tgtgttcctt tcattagaag tttgccatct ttaatcatta ttgtttcctt
     8401 tgcaactgat tctatatcgg aaattatatg agtagaaagt attattatct tatctctaga
     8461 catttttgct attaaattcc taaatctagc tctttcttgt gggtcaatac ctgctgttgg
     8521 ttcatcaaag actattatct tagggtcgtt tagtaaaact tgtgctatcc caactctcct
     8581 cttcatacct cctgaaaatt ttcctataga cttatttctc acatcgtaaa gacctactaa
     8641 atctaataat tctcctatct tactatttaa atattttccc cttaaacctt ttaaagctcc
     8701 aatatactgt aaaaattctt ttgctgtaaa atttttataa actccaaatt cttgtggcaa
     8761 atatcctaaa atatttctat aattagcatc cattttaaat atatcctcac cattataaag
     8821 tatctgacct tcatcaggtt ttatcaacgt tgttatttgt ttcattaatg tagattttcc
     8881 tgctccattt ggtcctagaa gaccataaac tccattttcc agagttactg taatcttgtc
     8941 gtttgctact tttttaccat atatctttga aagattttct atttctaatt tattcatctt
     9001 aactcccctt ttatctactt atataatctg attttattac taaaaaacat aatgacatag
     9061 cacctatcaa tattgccatc aaaagtccta ttgctatatt gacaatgtct atattcactt
     9121 taaatatcat aatatcacct agataatttc ctattatttt attaaaacca aatatcaaat
     9181 cataataatt tacatttgat acaactgaaa gtaatttttt aggtaatata tggattaccg
     9241 acattatctt agttatagat ataataaata taaaaaaact tattgaagat attgaagatt
     9301 tacttataaa agacccaaat atggctatca tggcccatcc ttcaaacatc aaaagctcta
     9361 tgcctatatt taatattaca tactttgtta tacttaaatt agttttcaat gcaactacat
     9421 tgtctactat tctaaaagat tctaagtctc cattaacagg tactccttgt tgcatacaag
     9481 ttataataaa tattcccatt aaataaacag aataaattac taatggaact aataaagcaa
     9541 caaatatttt agactttaat gcttttttct tctcttttga tgatagtatg atatctttta
     9601 ccgaagtaac gacttcatct acgtaaatat tggatataat tatagatata atgattaaca
     9661 ttcctacatt tataagtgaa tttgtaactc tatagttaaa tactttccaa aaactaatat
     9721 ccttatattc attcacactt agattatcaa gttttttctt tgacattaat tcaatttttt
     9781 tagaaaattc acctgtatct gtttgatttg aaaactgctt taaaacatta atcttatttt
     9841 gaaatttctc attacctatg tcgataccac ttctcgtatc ttctatacat cctttttctt
     9901 tatctatata ataacgttct gtttctaaaa caggcgtaat aaagatacta atcattagta
     9961 ttaacactaa tatccctaga gatataaaca tactcttaga tttagccaat ttttttattt
    10021 cccaatttac caatatattt ctcctcctaa atcattttaa ttacacatat atattaaaac
    10081 ttaaatctta tataaatata atactaacct taaataaagc ttaaattatt ttataattta
    10141 taggtgtgtt tattattgga gttacattta ttatttttct gggaaattat acattttcaa
    10201 ttatttttat tatttttgat atttttattg attggaaatt ttattgttgt tcaaattgta
    10261 gttattcttt aaatttaaag atggagtttt ttaagaatag aacctactgt ccttattatg
    10321 gaaagattat taaatagttg catataactt tatttatctt gaatcttatt tttaaaaaca
    10381 attataattt tagaatattt tctattctca aacaagcatg ttgaataatg aagca
//
LOCUS       DACTGW010000138        10401 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.138, whole genome shotgun sequence.
ACCESSION   DACTGW010000138 DACTGW010000000
VERSION     DACTGW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.138"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            252..2441
                     /locus_tag="I9573_002566"
     CDS             252..2441
                     /locus_tag="I9573_002566"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HAT4797176.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEEFIDIKTDLEKVTKANISIRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
     gene            2509..3276
                     /locus_tag="I9573_002567"
     CDS             2509..3276
                     /locus_tag="I9573_002567"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAT4797177.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            3334..4350
                     /locus_tag="I9573_002568"
     CDS             3334..4350
                     /locus_tag="I9573_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HAT4797178.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVYAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            4416..4664
                     /locus_tag="I9573_002569"
     CDS             4416..4664
                     /locus_tag="I9573_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797179.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELGVLLEEKKELEEE"
     gene            complement(4841..5338)
                     /locus_tag="I9573_002570"
     CDS             complement(4841..5338)
                     /locus_tag="I9573_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HAT4797180.1"
                     /translation="MYKIGESVMYPKEGACYISGLVTKDINHHIQKYYELTVIYNSNL
                     KISIPVLNADKIGVRPIMDENEVENFIQSLDKVDCLWVFDRKKRLKLYHDKFHSGDVF
                     EIVKLIKMLMIQDNSKQLCSTDKDFLNKAQRFALSELAAAQCKSYTIVLEEMKNHILN
                     SKNNN"
     gene            complement(5579..6643)
                     /locus_tag="I9573_002571"
     CDS             complement(5579..6643)
                     /locus_tag="I9573_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797181.1"
                     /translation="MIRLTIANIKRYIKNHALLVNMVLFPVILMFYLNFFTGNSENQS
                     MYFSPVAIVSNSSGKYEHKLIDSSKLKENSFGLNEQDKAMDLLKNNKVSAVFILDKNF
                     SSNIDNLKRPEVKCFKTENGGGSLWAESQIESFITNSLKLKIDRNIDDKLTKTNIIDN
                     SSGKNKNSFLIVFLICYFMYINAAHLASDLFSLKKSNTLKRLVSTKHNDIKIIFSIFL
                     GLFFTQAIVYTLTLVCFTSISNFKLTLNTLMIVLANSFVATGFVFWIARVFKNEGSIS
                     LMSTFYSLIGLAISISNMIPSLDKLSFMANLSKLTPFYWTIDAINNNGNILINIIVLI
                     LIGMVFVTAGSLNLRDFARN"
     gene            complement(6646..7743)
                     /locus_tag="I9573_002572"
     CDS             complement(6646..7743)
                     /locus_tag="I9573_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797182.1"
                     /translation="MKLYISVKTMLKVLKSSFMLNLIYFLALPLVLAGFLGMVTESIF
                     QNPIKTDLTSIVIYDKDNTKLSSNLTGYLKNDLSSILTVQKDDSKADLKLTIPKGYEN
                     NLLNEKSNNLSIEKLKSNDNIAILLQDILDTYHEKLYLNNSQKLSSEDFSKLFNKNSI
                     DTSIIKNNVKQNSYEYFALVSLGFFAIIFIMNSILSNYVSESKGLSKRLYSIPVTRVQ
                     FLIYDFIGFWIYSFIFLSLYVLFFRTLGVTFKGNFVILALLCALASYFMTSISTFITT
                     FFSKKYGTVIVYALLFLQTVFGGIFSIVNDVFTKFTSLSPTYLIGTLFSNYETFKTID
                     SIESLIFTCLIASTVLIALSIVKEKYKWREV"
     gene            complement(7757..8692)
                     /locus_tag="I9573_002573"
     CDS             complement(7757..8692)
                     /locus_tag="I9573_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797183.1"
                     /translation="MDIVRVNNITKRFNDKLALDNISFSVKEGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGDIQINGLDLSKDCIKAKSIIGLVPQELALIETLTPFDNLEYFGT
                     YYGLKGKLLQERITEALEVTGLTDVKKKKIKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKNISKKQKTTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVVSILENNSLLELGVDKKLQLTDV
                     LSVVDKNNSKITRISYEEPSLEDVFLNLTGKNLRD"
     gene            complement(8880..9524)
                     /locus_tag="I9573_002574"
     CDS             complement(8880..9524)
                     /locus_tag="I9573_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4797184.1"
                     /translation="MNKIKVLIVDDEKLIRKGLNIILSSYDDLEIVGDASNGYEALEF
                     CKTNDVDIVLMDIRMKVCDGVLGTRLIKEYDSSIALLILTTFNDDEYIEDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMYPQKTIEQKHISDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILGKLELRDRTQLVIFAFKNKIVI"
     gene            complement(9517..>10401)
                     /locus_tag="I9573_002575"
     CDS             complement(9517..>10401)
                     /locus_tag="I9573_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT4797185.1"
                     /translation="SFLIKEKYKYILFSLVIIFSFIIGLDKNLYLALESSSTLLIITL
                     FSVYLENENLSKLYNQSLYDKLRVSKDELKKANADLEIYANSIEELAILKERNRISRE
                     IHDSVGHSLSTTIIQLNAIEKLLKDKPLISDLVHELREFVTESFQDVRRAISELKPIE
                     YENYQNLLKIRELVNSFIKLTNINVRLTISKNTWNLSTNQSIALYRLVQESLSNASRH
                     GKATEIRIFITFNPSNLIITISDNGIGCNNIKRGNGLSSISERIYELNGKVEFDNSNT
                     GFTIRASFPKFSGGDFFE"
BASE COUNT         3961 a         1222 c         1640 g         3578 t
ORIGIN      
        1 aatataaaaa aataaagtat ataagagtat aatttgactt ttatatactt ttttatatta
       61 tttttgagtt ttttaggaag atattttaca agttaagaaa actataagga gtgttatatt
      121 gaatataaaa tagtataata tatattatgt tagataatta atatgaaaat atacaaatat
      181 ataaaaaagt ttgtttagag ttatagttaa tgtgtgtatt aatttaatac taatttattg
      241 gaggtttaaa aatgaaatac ataataaaaa gagatggtac aaaagaactt tttagtaaaa
      301 gtaagattga agatgctgtc tataaagcta gtataaatag tgaaggtgga gtagatagag
      361 agttggcata tgatatatca agaaaaatag ctaatgacta taattctatg gaaaaatgct
      421 tatccgtaga agaaatacaa gatatagttg aagtactact tatggaatca aatagaaaag
      481 atatagcaaa acactacata ttatatagag aaagacgctc tgaaataagg actaaagctt
      541 gggagtttga tgaattacaa aaatcaatat ggaagaacaa gtatagacat aatggtgaga
      601 gttttgatga atggataaag agaatttcaa ataatagttc aagaatatca aaactaataa
      661 gacaaaaaag atttctattt gctggaagaa ttttagcaaa tagaggatta cagaaagaag
      721 gaaaaaaagt aacatattct aattgttatg taatagaacc accaaaagat agcatagaag
      781 atatatttga tactgcaaaa aaacttgcta gaacttttag ttatggtgga ggagttggaa
      841 ttgatatttc aaagctaaga ccaaaaggag cgaaagttaa caatgctgca aaacatacga
      901 ctggagcagt ctcttttatg gaactttaca gtatgacaac tggcttaatt ggtcaaagag
      961 ggagaagagg agctcttatg atttctatgg atgtaaatca tccggagata gaagagttta
     1021 tagatataaa aacagatttg gagaaggtta ctaaagcaaa tatttccata agggtaacag
     1081 atgaatttat gcaagctgta agggaaaaaa agttatataa ttgtactttt gaagttgaaa
     1141 ctactggaga aaaaatagtt aagatagtaa atgcttatga tttatttaat aaatttgtga
     1201 aaaataactg ggattatgca gaaccaggta tattgttttg ggataaaata aaaacatata
     1261 attttatgag tgaagataag aacttttgct tttcaggagt aaatccttgt gcagaagaac
     1321 cacttccaag tggaggaagt tgtctacttg gctcaataaa cttgtcagag tttataatac
     1381 aaccatttgg tgaacatgct acttttgata ttaataaatt aaaatcttgc gttagagaag
     1441 ctgtagtagc tttaaatgat gtcttagatg aggggttgaa cttgcatcca ttacaagaac
     1501 aaagagatag tgtacataag tatagacaga taggtttagg tgtaatgggt atagctgata
     1561 tgcttataaa aatgggaata agatatggtt ctgatgaatc tttagaacta tgtgatttaa
     1621 ttggtaagac tatgttaaat gaagctgtga agcaatcagc attaattgct aaagaatatg
     1681 gaacatttag tgagtataaa tatgaaaata tttctaaatc taagtttttt atagaaaatc
     1741 taaatgaaga cactaaagaa atggttaaaa agtatgggct tagaaattca caacttttaa
     1801 ccatacctcc tacaggttct atatctacaa tgataggtat cagtggtgga atagaaccta
     1861 tatttaatct ttcatatata agaaaaactg aaagcttgca tgatgaggat gtgtattata
     1921 aagtatatac tccaattgtt aaagagtata tggacaagaa aggtattaca gatgaagaat
     1981 gtttaccaga tttttttgtt actgctatga atataggata tgaagaaaga ataaacatgc
     2041 aaaaagtatg gcaaaagtat attgatgctt ctatatcatc tacaataaat ataccttttg
     2101 aaactacagt tgaagatgtt ttcaatatat atattaaggc atgggaggct ggtcttaaag
     2161 gagtaacaat atttagagat ggatgtaaaa ggagtggaat tttaataaat gaaaaaaagg
     2221 aagaaaataa taaagaagat aaaaaagaaa atagtaaaaa agaatattat aaaaataata
     2281 aaaaagaaaa agataataaa gatgaaaaag ccaaagaaat aataaataaa aattttgaag
     2341 aagatgaaaa atttgtgtgc cctgaatgtg gaagtgaagg aattgcacat acaggtggat
     2401 gtagtatatg tttaaaatgt ggctatagtg gatgcaatta aactctaatt aaaaaatttt
     2461 aatatattta aaaattaaat ttttaaaaac gaaagatgag ggaatggtat gcaggataaa
     2521 agtgtgaggg agattaaaga aataatagag actttagaag tagaaaaata tatggaatat
     2581 atagaactgt taagagttga tgaaagaaaa tctgtacaag gtctagctat aaaactagca
     2641 aaaaagctgg ataatattag aaaagaagaa gaaagattgg aaactataaa tatatttgag
     2701 aacgaaggct atgataaagg ttatttgtat attggaggaa ttgatgaggc gggaagaggg
     2761 ccacttgctg gacctgttgt agcttctgtt gtagtattta aaaaagatac aaaaatagaa
     2821 ggtgtaaatg actctaagaa actgagtgag gcaaaaagag atgaactttt tgaagtaata
     2881 aaagaagaag cactagatta tggaataggt atagtaaata atgaagaaat agatgaattt
     2941 aatatattaa atgctacata tatggcaatg aaaaaggcta taaattgttt aaaaaaagca
     3001 ccggattatt tattagttga tgcagcaaca ataccaggta ttgacatatc tcaaaatcca
     3061 attgtgaaag gagattcaaa atctatatca atagcagcag caagtatact tgctaaagtt
     3121 actagggata gtataatgta ccaatatgat agagtatacc cagaatatgg gtttaaatct
     3181 cacaaaggat atggaactaa agaacactat gaagctattg aaaaatatgg cataactcct
     3241 atccacagaa aaagtttttt gaaaaatata ctgtaaatat aatatgatat aatgtatata
     3301 atataaatta ctatagttaa tgggggatta aagatggaat ataatgaatt attaaaaagt
     3361 gctagagaaa atttaaatgg aagttgtaaa gtttgtaagg tttgtaatgg ggttgcttgt
     3421 gcaggtgaag tgcctggcat gggaggtaaa ggaagtggtt cttcttttat agaaaatagc
     3481 aagagtttag aaaaagtaaa agtaaatatg agagtaatac atgatgtatc aaatcctgat
     3541 acatctatag agatgtttgg aaagaaaatg agtgcaccta tatttgcagc tccagtaaca
     3601 ggaactactt taaatatggg tggaaaaatt aatgaaagag attacataga accagtagtt
     3661 gcaggatgtg ctaatagtgg tatttatgca atggttggtg atacggcagt agatgctttt
     3721 ttaatggaaa atttagatgt agtcaaaaag tataatggag ctggaattgt atttattaaa
     3781 ccttgggata atgaaaatat tataaaaaag ataagattag ctgaagaagc tggtgcattt
     3841 gcagttggag ttgatataga tgcctgtggt ctagtaactt tatcattaca tggaaaacca
     3901 gtacttccta aaaatgttaa acaaataaag gagttagtaa agtctactaa actaccattt
     3961 atattgaaag gtataatgac tgttgaagat gctttaatgg cagtagaagc aggtgtgtat
     4021 gcaatagttg tatcaaatca tggaggaaga gtgcttgact gtacaccagg agcatgtgaa
     4081 gttttaccaa aaattgctga tgctgttaaa ggtaaagtta caatacttgc agatggtgga
     4141 gtgagaactg gtttagacgt actaaaaatg ataggtttag gtgcagatgc agtattaata
     4201 ggaagacctt ttgtgactgc ttcatttggt ggtgcaactg atggtgtaga aacttatgta
     4261 aataaacttc aatcagagct aagtagttct atgatattaa ctggatgtca aactataaaa
     4321 gatattgatg gaaaagttat atacaaataa aattttatac aaaaaagagg aaaaatcatt
     4381 ttaatgtaga ataattgtac aaagggggaa tttatatgag tagaaaagct tatgaagaag
     4441 ccttagtaga attagaaaaa tttattgatg aaagaaaaga aataattaaa agtgcggagg
     4501 actgtattga taagtacata gttgacagaa cgctaccttt tgattacaaa gataaatgtg
     4561 tggaatggca acaagagtta cttgatattg cagaggcaca agttttagaa gcaaacgaat
     4621 taggtgtttt actggaagag aaaaaagaac ttgaggaaga gtaagtttta ttactaaatc
     4681 tagggaaaat caagaatatg atttacccct agattttttt atttaaataa agatgtctat
     4741 ataattaatc tatctttatc aatgttagat taaaaggttt gaagaaaaat agagtaattg
     4801 tatgaatagt taaacaatta aactagtcta taagaccaat ttaattgtta ttttttgaat
     4861 ttagtatatg gtttttcatt tcttctaata caattgtgta ggatttgcat tgtgctgcag
     4921 ccaattctga caaagcaaac ctttgagcct tatttagaaa gtccttatct gtgctacata
     4981 gttgttttga attatcttgt atcataagca tcttaattaa ttttacaatc tcaaaaacat
     5041 ctcctgaatg aaatttatca tggtatagtt taagtcgttt ttttctatca aaaacccata
     5101 aacaatcaac cttatcaagg gattggataa agttttcaac ttcattttca tccataattg
     5161 gacgtacacc aatcttatca gcatttaaaa ctggaatcga aattttaaga ttactattgt
     5221 aaataacagt caattcatag tatttctgta tatgatgatt tatatccttt gttacaagac
     5281 cacttatata acaggctcct tcttttggat acattacact ttcaccgatt ttatacatat
     5341 ggttatacct ccaaactgaa ttttagatat tgcaatttgg gtacaatgct ttaattatct
     5401 aataattaat attatatcac gaaatgcttg acaagtcaaa aagattaata tataataaaa
     5461 agtttagaat aatagatata tataattggc tataaaaata taagataaga ggatttaata
     5521 tattaaaata tctagttagt attgtataaa ataacatact aactagattt aataattatt
     5581 aatttctagc aaaatctctt aagttaagac ttccggctgt tacaaatacc attccaataa
     5641 gaattaaaac aattatattt attaatatat taccattatt attaatagca tctatagtcc
     5701 aataaaatgg tgtaagcttt gaaagattag ccataaagga taatttatca agtgatggta
     5761 tcatattaga tatggatata gctaaaccta ttaaagaata aaacgtagac ataagactta
     5821 tagaaccttc gtttttaaac accctagcta tccaaaatac aaatccagta gcaacaaaac
     5881 tatttgctag tacaatcatc aatgtattta gagtcaattt aaaattactt atagatgtga
     5941 aacatactaa agttaaggtg tatacaattg cttgagtaaa aaataaccct aaaaatatgc
     6001 tgaaaattat ttttatatca ttatgctttg tagaaacaag tctttttaag gtattagatt
     6061 tctttaatga aaataaatca gaagctagat gtgctgcatt aatgtacatg aaataacata
     6121 tcaagaaaac tattaaaaat gaatttttat tcttacctga actgttatca attatatttg
     6181 tcttggtcaa tttatcatct atatttctat ctatttttaa ttttaatgaa ttagtaataa
     6241 aagattctat ttgtgattct gcccaaagac tacctccacc attttcagtt ttaaaacatt
     6301 taacctcagg tcgttttaag ttatctatat tgcttgaaaa gtttttatct aaaatgaaga
     6361 cagcagaaac tttattattt tttaatagat ccatagcctt gtcttgttcg tttaatccaa
     6421 atgagttttc ttttaattta gaactatcta ttaacttatg ttcgtatttt ccagaagaat
     6481 tggatacaat cgctactgga gaaaagtaca ttgattgatt ttctgagttg ccagtaaaaa
     6541 agttaagata aaacattaat ataacaggaa acaaaaccat attaactaaa agagcatgat
     6601 tttttatata tctttttatg tttgcaatag tcaacctaat cattattaaa cctccctcca
     6661 tttatatttt tcttttacaa tagaaagtgc aatcaatact gtagatgcaa ttaggcatgt
     6721 aaaaattaaa ctttctattg aatctatagt tttaaacgtc tcataattac tgaacaatgt
     6781 tcctattaaa taagtaggag ataaacttgt aaacttagta aacacatcat ttactatgct
     6841 gaaaatacct ccaaaaactg tttgcaagaa gagtaatgca taaactataa ctgtaccata
     6901 tttcttgcta aaaaaggtag ttataaaagt agatattgaa gtcataaaat aagaagctaa
     6961 agcacaaagt aaagctaata taacaaagtt acctttgaat gttacaccga gtgttctaaa
     7021 aaataaaacg tataatgata aaaatataaa tgagtaaatc cagaaaccta taaaatcata
     7081 aattaaaaat tgtactctag ttacaggtat agagtagagt ctttttgaaa gtccttttga
     7141 ttcacttaca taatttgata aaatgctatt cattataaaa attattgcga agaatccaag
     7201 ggaaactaaa gcaaagtact cataagagtt ttgtttaaca ttatttttta ttatagaggt
     7261 atcaatagaa ttcttattaa ataattttga gaaatcttct gaacttaatt tttgagaatt
     7321 attcagatat aatttttcat gataagtatc taatatatct tgaagtaata ttgcaatatt
     7381 atcattagat ttaagttttt ctatacttag attatttgat ttttcattta ataaattatt
     7441 ctcatatcct ttaggtatag taagttttaa gtctgcctta gaatcatctt tttgaactgt
     7501 taatatagaa gataaatcgt tttttagata tcctgtcaaa ttacttgata gctttgtatt
     7561 atctttatca tatataacaa ttgatgtcaa atctgttttt ataggatttt gaaatatact
     7621 ttctgtcacc atcccaagaa atccagctaa aactagaggt aaggctaaga aatatattaa
     7681 attaagcata aaggagcttt ttaaaacttt tagcatagtt tttacactaa tgtacaattt
     7741 catgaaatca cctccattag tcccttaaat ttttacctgt tagatttaaa aatacgtctt
     7801 ctaaactagg ttcttcataa gatattcgtg ttatttttga attatttttg tcaacaacag
     7861 ataatacatc tgtaagttgt aattttttgt ctacacctaa ttctaataat gaattatttt
     7921 ctaaaataga tacaacacct ttagtatttt ttaaatcaaa tataagttga gcatttatat
     7981 tttttatttc cataattaat tttgtatttg tagatactaa tgattttaaa taatcattat
     8041 ctccaaaagc aatctcttta ccttcatcca taataaaaat cttagaacaa agatgctcaa
     8101 cttcttccat ataatgagaa gtatatatta ctgtggtttt ttgcttttta gatatatttt
     8161 ttataaaatt gaaaatatgg tttcttgatt gcgggtcaac acctacagtt ggttcatcca
     8221 atattaatat tttaggatga tttaaaagtg ctattccaat gttcaatctc ctttgcatac
     8281 caccagataa ttttttgatt tttttctttt taacatcagt gagtcctgtg acttctaagg
     8341 cttcagttat tctctcttga agcaattttc cctttagtcc atagtaagtg ccaaagtact
     8401 ctaagttatc aaaaggagta agagtctcta taagtgctaa ttcttgtgga actaatccta
     8461 ttatagactt tgcttttata caatcttttg acaaatctaa accattgatt tggatgtcgc
     8521 cactatttgg taacataaga tttgtaatta tatttataag tgtagattta ccagcaccat
     8581 tcggtccaat aagtccaaat atctctcctt ctttaacaga aaagctgata ttatcaagtg
     8641 ctaatttatc attaaatctt tttgttatat tatttactct tactatatcc ataataattc
     8701 ctcccaaaat aattttgtat aagttaaaaa tctataatac tataataaag aattatgaat
     8761 cataattcta ttactataag tcactaaaaa aagtgactta agtcacttta tattttcatt
     8821 tattataaat gagtttgtga gtaagaaatt tctagggtat agatttctta cctatatatt
     8881 tatattacaa ttttattttt gaaggcaaat ataactagtt gtgtcctatc acgaagctct
     8941 agtttcccta agatattggt gatattattc tttattgtac cttcagataa gaataattct
     9001 tggctgattt ctttgttgtt aagtccattt gcgattaatc taattatgga tatttctttt
     9061 tctgttaaat tatacatatc agaaatatgt ttttgttcta tagttttttg agggtacata
     9121 attttttcag ccacactttt atcaagtaca atatttccaa aaaacgagga acgaatccct
     9181 tcatgtaata cttcatccga actatccttt aaaagatatc ctgaagcccc aaatttcata
     9241 gcatcttcta tgtactcatc atcattaaag gttgtcaaaa ttaataatgc tatagagcta
     9301 tcatattctt ttataagtct tgtgcctaga actccatcac aaactttcat ccgaatatcc
     9361 ataagaacta tgtctacatc atttgtttta caaaattcta atgcttcata tccattggaa
     9421 gcatctccta caatttctaa atcgtcataa gatgataaaa taatgttaag accttttcta
     9481 atcaattttt catcatctac tataagaact ttgattttat tcaaaaaaat cacctcctga
     9541 aaatttagga aaagacgctc taattgtgaa tccagtatta gaattatcga attcaacttt
     9601 accatttaat tcatatattc tttcgctaat actgctaagt ccatttcctc tttttatatt
     9661 gttacaccct ataccattat cgctgatagt aattataaga tttgaaggat taaatgttat
     9721 aaaaattctg atttcagttg cttttccatg tcttgatgcg ttggaaagag attcctgaac
     9781 aagtctatat aaagcgatag attgatttgt agataggttc caggtatttt ttgatatagt
     9841 taatctaacg ttgatatttg taagtttaat aaagctatta accagttctc tgattttaag
     9901 taaattttgg taattttcat attcaatggg ttttagttca gatatagctc ttctcacatc
     9961 ttgaaaactt tcagttacaa actctctaag ttcatgaact aaatcagaaa tgagaggttt
    10021 gtcttttaag agtttttcta tagcatttaa ttggattatc gtcgtagaaa gagaatgtcc
    10081 cacactatcg tgtatttccc tcgatatacg gtttctttct ttcaaaattg ctaactcttc
    10141 tatagaattt gcataaatct ctaaatcggc attagctttt tttaattcat ctttagaaac
    10201 cctgagtttg tcataaagac tttgattgta taatttagat aaattctcgt tttctagata
    10261 cacagaaaag agggttataa ttagtaatgt agaacttgat tctagtgcta aatataaatt
    10321 tttatctaaa ccaataataa aagaaaatat tataactaaa gagaataata tatatttata
    10381 tttttccttt attaaaaatg a
//
LOCUS       DACTGW010000139        10340 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.139, whole genome shotgun sequence.
ACCESSION   DACTGW010000139 DACTGW010000000
VERSION     DACTGW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.139"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            74..1867
                     /locus_tag="I9573_002576"
     CDS             74..1867
                     /locus_tag="I9573_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAT4797186.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILDELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(1948..3330)
                     /locus_tag="I9573_002577"
     CDS             complement(1948..3330)
                     /locus_tag="I9573_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAT4797187.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            3588..3935
                     /locus_tag="I9573_002578"
     CDS             3588..3935
                     /locus_tag="I9573_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAT4797188.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            3953..4987
                     /locus_tag="I9573_002579"
     CDS             3953..4987
                     /locus_tag="I9573_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797189.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVVWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(5036..6085)
                     /locus_tag="I9573_002580"
     CDS             complement(5036..6085)
                     /locus_tag="I9573_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT4797190.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENRVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            6360..7895
                     /locus_tag="I9573_002581"
     CDS             6360..7895
                     /locus_tag="I9573_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797191.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFNLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(8081..8767)
                     /locus_tag="I9573_002582"
     CDS             complement(8081..8767)
                     /locus_tag="I9573_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HAT4797192.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEGVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            8892..10274
                     /locus_tag="I9573_002583"
     CDS             8892..10274
                     /locus_tag="I9573_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAT4797193.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
BASE COUNT         3995 a         1287 c         1694 g         3364 t
ORIGIN      
        1 aaatatttat gtaataaact ttacatactt atatatttag ttattcaaac tattatttcg
       61 aggaggtctt gttgtgaata taaaagatag attaagtgga ttaagaaaat ttatggaaga
      121 aaaaaatata gatgcctata tgattccatc ttctgataat caccaaagtg aatatgtagg
      181 agattacttc aaatcaagag aatttatatc agggtttaat ggttcagcag ggacggttat
      241 tgtgactaaa gacgaagctg ggttatggac cgatggaaga tattttatac aggcagaatc
      301 tcaattagaa ggaagtacaa taaaattatt caaaatggga caagaaggtt gcccaactac
      361 agatgaatat ttatataaaa atattcctga aggaggaact ttaggatttg atggtagagt
      421 tatatctgct agagaaggag ctacattagc tgaaaaatta tctaaaaaag gtataaagat
      481 agaatatcag tatgatttaa tagatggtat atggccagat agacctgctt tatcagatag
      541 taaagcattt ttattagatg ttaaatattg tggagaaagt tttagctcaa aattagctag
      601 attaagagaa aaaatgagtg aaaaaggtac ttctactcat gtaataacta ctcttgatga
      661 tatagcatgg ttattcaata taagaggtgg agatgttaaa tataatccag ttgtactttc
      721 ttatgcagta ataactttaa aagaagttta tttatttgtt gatgaaagta agttaaatga
      781 agaaatatta gatgaactag ctaaggaaaa tgttcaaata aagccatata atgatgtgta
      841 tgaatttgta aaaaatatag acaaaactga aaaagtttta ttagatggaa ctaagcttag
      901 ttatacaatt tacaataata taccttgtga agttgagaaa gttgatgagt tcaatccagt
      961 aatgtttttt aaagctcaaa aaaatgaggt agaattagaa aatataagaa attcacatgt
     1021 aaaagatggt gtggctttca ctaaatttat gtactggtta aagaaaaatg taggtaagat
     1081 ggaaatcact gaaataagtg ctactcaaaa attagaggac ttaagaagag agcaagaagg
     1141 tttctttgag ccaagtttca atacaattgc tgcatataaa gagcatgctg caatgatgca
     1201 ctatagtgca actccagagt caaattataa attagaggca gaaggattat ttttagtaga
     1261 ttcaggtgga caatattatg atggaactac tgatataact aggactacag ttttaggacc
     1321 tataagtgat gaattaaaac tacactttac ttctgtagca agaggaatga taaatctttc
     1381 taaggctaag ttcttgcatg gttgtagagg atataactta gacatactat ctagaagttg
     1441 catgtggaat atgggaattg attatcaatg tggaactgga catggtatag gatttgtgct
     1501 aaatgttcac gaagctccaa atggatttag atggagagta gtacctgaaa gatttgatag
     1561 tgcagttctt gaagaaggaa tggtaactac taatgaacca ggtatatata tagaaggttc
     1621 tcatggtata agaacagaga atgaaattgt agttagaaaa gctgaaaaga atttctatgg
     1681 tcaatttatg gaatttgaag ttgttacgtt agctcctatt gacttagatg gtatagtacc
     1741 agaattaatg aataaggatg aaaaagatta tttaaactgg tatcataagt tagtttatga
     1801 taaaatatca cctttcttaa ctgatgaaga aagagaatgg ttaaaagttt acactagagc
     1861 tatatagttt gtactagata caataaaaat cgaggtatta taatgcctcg attttttatt
     1921 gtatatttta ttaactttaa aaaattttta atttgataat tctactaatt ttttattcat
     1981 tataagtagt ataattccaa atatcaataa aaatgatggt atcactatga agacttgtaa
     2041 cataccaagt tggtctataa tagcttgcgt atagccagat aatttatttg ctgcaaattt
     2101 gcctaaaaac caaacaccca ttaggaatga aatatacttt tcaggcgaaa gtttatttac
     2161 cataccatac ccgactggag aaaagcaaat ttctcctata ctttggaatg ttaaaaatcc
     2221 aactacccaa aatacacttg ctttgtgaat tgaattaggg tctgttcctg tttgtataac
     2281 tgctactatc ataaacaaaa atgctatagc taatagtata aaacctaagc tcattttttt
     2341 tggcacactt aaatcgcctt ttttagtttg agataatttt acccatacta aagcagagat
     2401 aggtccaaga ataacacaaa ggagaccatt gtatgaatct atccatgaag taggaatttg
     2461 aaaattgcca acagtaagat ttatgtgttc ctttgtatag agtgcaatag acatagaggc
     2521 ttggttatat gctatccaaa aaaatactgt gaaaaaagct aacaccaata ttactattat
     2581 tctttttttc tcaatcaaag ttaaaggtct tgaagcgagc attttcttat attctagtga
     2641 ttttcttata gactctctcc cgttactagg gtcattattc aaaggtttat aaaactttct
     2701 gtttccaaat ataaagaaga ataaacctat tagaaccgta attgatgaaa ataaaaatac
     2761 atatctatat ccatacatac ttatagataa tccgcttttc atagcaaacc attcattagc
     2821 aactaaaccg catattaaag ggccaaataa agtacctaga ttagtagcca tatacgtaat
     2881 agagaaagct ccatcttttc ttgggtcatc attggtatat aagtttccaa ccatagtatg
     2941 taagtttcct ttataaaaac ctattcctaa tccagataaa aatatcatga tgtttatatc
     3001 catcttacta tttgctatgt atcctagaaa ataaccaatt gaaaccatta gcattccaag
     3061 tataatacag tttttgggac ctaaaaatct atcactgacc catccaccaa taactggaga
     3121 gaaataagtc catgctaaaa aagatgcaag cattgatgcg gattctgctg gtgttaaccc
     3181 taaaccacct ttggagactt gttcagctaa aaacaataat aataccaatt tcataccata
     3241 gtatgcaagt cctacaaagg taaatattga acagcagaaa tataaatctc taggttgttt
     3301 aagtttttct tgcgttttta aaactgacat atgtcgagaa cctccagata aatagtttaa
     3361 caaaatttta ccatactata tgagtatatg tttttttcat atagacgtca acttgtaaga
     3421 taatttaatt aagtcgaaat atatgtaaaa atagtatcat ttttgtgtat tcagtaatat
     3481 ttttttatga taatatatgt tattattata ttaaaataca aacatattta tggaaaggat
     3541 atgccaattt agtagttaga ttgattgttt ggcaggtggg atgagctatg ataagaaaga
     3601 aatctgaatt acaagtagaa gtaaaagaaa atttaaatgg tggtatagga aaggttaagc
     3661 ttgaaaacat aatacaaaat gaagagctta aaggaaaagg aagactattt aaaagagtag
     3721 ctttaccagt aggaagttca attggtgtgc atgaccatac aacagatttt gaagtatatt
     3781 atatactaaa gggaaaaggt aaagtatttg ataatgggga atttgtagaa gtaaatgagg
     3841 gtgatgtagt gtatacagct gatggcgaaa aacattctat agaaaatata ggagaagaag
     3901 atcttgagtt tgtagcacta gttttatata tgtaaaatat ggaggaggag tcatgacagg
     3961 caagatagaa gaaattaagg acaagataaa tagattttgc aactccaatt taaatcaaga
     4021 atacaaagat ataagcttta aaattttgca aaacctttta gataataata aagagataat
     4081 tcattcttct agagctgata tatggtcttc agctatacta aatatagttt tagaacaaaa
     4141 tcttttatac aataagaagc atccactaca tataacaaaa aaagagtttt caaaacaaat
     4201 aggtgtttct ttaaatacta taaataataa atcgtcttta ataaaagatg tttgtagtat
     4261 tgacttttta aatcaagata ctatagctat ttctaatgta gaattttggg taaaagagac
     4321 caattccaaa tcaaagtctg tagatttaga tttagaaaag aaaaaaatac tatataaaag
     4381 atatattaag ctatcacaag atgcgaatag tgatattgaa tcaattagat atatggagga
     4441 agctgtaaat ataggtaaaa gtatgattac taaagaagat aaagaacttg gattttggaa
     4501 aggtttatct accagagcct atatgatagc acttgaatcc cttgctagaa agttagaaag
     4561 tattaataat ctaaaggagt ccaaaaaagt attagaatat ttaatagaaa ttaatcctag
     4621 tgatgagcag ggaataagat acaaattatt aaatgtatta attagattaa atgatagaac
     4681 ttcaataaat aaattatttg aactttataa agaagaaaaa tctgctacat ggatgtacag
     4741 taaagctctt tattacttta aaaataaaag tatgtttttg gcaaatgatt ctattaaggt
     4801 tgctagaagt aaaaataagt acgttggatt atatttgtta gattggcgaa atgcttttgg
     4861 tagagaattt gttacagagg aagaaaaggc agaagctgtc tattactatg atgaaaacat
     4921 agtggtttgg aatgaagtta aaggttctat ggattggcta ttaaaaaaaa tgttggaatt
     4981 ttcataaaaa aaactactcc ttacttttat ataagtaaaa ggagtagttt ttaatttata
     5041 catctagttt taaatcttca ggttttatta gacctttttt tctcataaat tctgaaatca
     5101 acaaagttac aatagcagga agtataaaat taagtaaaag tataccaacc cacatagata
     5161 tacctccttt tccagcactt tgcatagcag ttactgttcc aaattgtcca accaatccac
     5221 aagtacccat accagaacca ataggaatat tctccatttt gaatattgtt gtagataatg
     5281 gtccaagtaa tattgaagct acagtaggtg gtatccaaat tttccaattt tttattatat
     5341 taggtacttg caacatagat gtcccaagac cttgtgccac aaggccacca actctatttt
     5401 ctctaaagct cataacagca aagcctacca tttggcaaca acaacctaca gttgctgcac
     5461 caccagcaag accacctaaa cttaacatca tacaaatagc tgcactactt ataggtaatg
     5521 taagagctat tccaacaagt gcagagacta aagctcccat tattataggt tgcatagttg
     5581 tagcttgcat tattatattt ccaaatgaag tcataaaagc agaaacagct ggaccaataa
     5641 gttcagctac aagtactcca attagtattg tcacagcagg tgtaaccaat atatctattt
     5701 ttgtttcttt tgaaactaat tttccaacct ctacagcaaa aagtgtagcg acaaatgctc
     5761 caacaggtcc tcctaattca ttaccacaag ctccaacaag tgcacaagaa aatagtacaa
     5821 gtggaggtgc ttgaagtcca tacgcaatag ccactgctat agctggacca gtaacctgtt
     5881 tagcaagagg gcttataact tcagtaaaaa gagaaatatt gaaattatct cctaatgtat
     5941 tgaaaattgt tccaattaaa agggacgaaa aaagaccaag tgccatataa cttagtgcgt
     6001 caataagata cctttgaaca gaaatttcaa tattttttct tttagcaaat gatttgaaat
     6061 ttcttttttt ataattgatt tccaatttaa aatcctcctc atataatata agtataattt
     6121 ctttttaatt aattatataa cttttcagct gacaagacaa atgttatttc actaaaatta
     6181 gactattgta atagtaagaa attaacatta attttagttt taaattttaa atttatggtt
     6241 tataattagt atagggatat tctgaaatat aataattttc cagtttatcc caatatttaa
     6301 cttaggtaga ttgcttacaa aacaaatgat tatatattag gctaaaagga agtggataga
     6361 tgatatttgt aaaaaataag aaatacagaa aaattgccat aattatatct attcctatat
     6421 tgcttgttgc gcagtactac ctttttaaat ttggaatact tagaccaatg gtaaagggag
     6481 tagaaattga aattgtagat ggggaatatg ttaaagatat agataaatat gtagtaaaat
     6541 taaatgaaac agttactttg tctacaggtg aatatattaa ggttccttcg tatgctaaaa
     6601 atccaaatat ttggtttaat gtattggata actcagggac tttaaaaata gatggaaata
     6661 aaatgacagc attaaaggtt gggtattctt ctgtgggtat catgaaaaat tcaagagtat
     6721 taaaaaaggc tactataaaa gtagtagacc cagaggtaga atcactagat atggagatta
     6781 atggggattt aaaacatgtt ggtgatagtg ctactataga aagtacaata gaagttgatt
     6841 atgataagtt taaaaaatca tataagccag tttataagag tagcaatgaa aatattttaa
     6901 aaatcaaagg aaataaagta agtgcagttg gagttggtaa agctacaata tatgctaagt
     6961 gtggaaataa agaaattgag agaacattta ggattcaagc tagagttgct gaaataaata
     7021 tggatgatat aaaaatagaa gttgaccaaa atgaaaaatt gaaaccagat ataataactt
     7081 ctccacctgg tttagaacca ccaaaaatat attatgagtt agtagattca aaattacctg
     7141 tagaaagagc aataagttta agtagtaatg gaaatgttgt aggtcttaga gaaggtaaag
     7201 atagggtgag aataacttgt ggagataaat ctaaagtaat aacagtaaca gtagtcaaag
     7261 aaactataac gaatgactat atacaaaact tggttggttc acataaaata gtaaacaata
     7321 aagttattat taaacttgaa tgggattata tgaaagatgt ctttgattat gatatatatc
     7381 ttaaagataa gttgtcagga gaatcattta agaaaattaa atctataact ataaatgaaa
     7441 gtgatttagg taagtctaac aaggtatatg caactataga agtagatatg aagggtaatc
     7501 aaaatataga ttttgatatt tatgtcatag ggaaaaagga ttcagataca actaagccaa
     7561 gtaatgtaat aaatattact aatggaaatg gaaattcaaa taatgataat aatattaaag
     7621 atatgagggt agaaaatata tcagctaatg ttgatagaga taataatgtc attagagtta
     7681 actggagtag tatatctcat aaggactgta cttacagtat atatgtaaaa aataatacta
     7741 atggagatgg agaatttaat ctatatcaaa gtgatataca gggaaatgag catactataa
     7801 gtattcctga aggagattta aatctagata tttatgttgt tgcaaattat aatggaaata
     7861 gctctcaaaa tagtgatgtt ataaatgtaa gatagatttg tatacaaatt gaaagtgttg
     7921 agtttattta aatattaaat taagaaaaag gatagtatag ggtaattata ctatcctttt
     7981 tttgttacaa tatataggtt taaaatctct agtaatataa attataaaaa gtcttattta
     8041 cacaaatcta tagatatttt ttttattaat caatattgtc ttaaattgaa cttagtgcgg
     8101 cttctattgc atgtattact gtcgtatcaa atagtggaat agaagtgtca ttttgtttaa
     8161 ttaaaagacc tatttctgta catcctaata taacaccctc tgcaccttgt ttgccaagct
     8221 tatctataat agataagaaa gctttttttg aagattcaga tataattcct aagcaaagtt
     8281 cgttaaatat tgtgtcattt actattatac ggtcttcttc atttggaatt aagacttcaa
     8341 ttccattatt aataattaca tttttataaa aatcttgttc cattgtatat ttagtaccta
     8401 ataaggccac tttttttatt tctttttccc ttaatactgt agcagttaca tctgctatgt
     8461 gaagtaatgg aatatgtata ctttcttgta ttttatctga taccttatgc atagtattag
     8521 tacagataac aataaaatct gcccctgcct cttgtaattt aattgcagca tcagctaaaa
     8581 ttttggcgct tttttcccaa tttccactag attggcattc ttcaatttct tgaaaatcta
     8641 cactgtaaag tatacattta gaagagtgta gtccacctaa tctttctttt attactgtat
     8701 taataacttg ataatatgta atggtacttt cccaactcat accaccaatt aagcctatag
     8761 tcttcatatt atacctctct tatttattta ttttgaaata gctatttaca aatacaagta
     8821 tagcatctaa aatagaagca tacaataata aaattgtatg gagtacaatt ataattaaga
     8881 ggagtaatta tatgccaata aattcttttg aaaactatcc tatgacatgg aaaccaaaat
     8941 tagataaccg aaaaccacca atatataaaa ctttagcaat gttattagaa gaagatatta
     9001 aaagaggaaa tttaaatcct ggagataaac tgcctccaca acgggaatta gcagattttc
     9061 tagatttaaa cttaagtaca ataacacgag catttaaact ttgcgaagaa aagggactaa
     9121 tatgtgcaaa agtgggaaag ggaacattca tttcttctga tgtaaatgta agtaatacat
     9181 tattatatca aacggaatca aaagacataa ttgaattagg gacagttcat cctccatatg
     9241 agcaaaatac atatattatt gactttatta aaaatgtact aaaacaacca gaaatggata
     9301 ggtttttaca atacatgtct ccatctggca catatatgca aaaaaagtca attgcaaaat
     9361 ggatagaaag aaataatgta tacaccagtg aagaaaatat attattatct acaggagggc
     9421 aaaatgctat atgtgcaact ttactaggat tatttaaagc tggtgataga attgctacag
     9481 attctttaag tttttctgga attaagtcaa tagctaaaat gataggtatt caattagtcc
     9541 caattcctca agaaaataat gaaatatcga ttgaatatct tgaaagttat tgtaaaaatg
     9601 aaaatataaa aggcatatat cttataccag attatcataa tccaacgaca catactatgt
     9661 ctgatttatc tcgtaaaaag atagccaaga tagcaaagca atataatata ataattattg
     9721 aagatgctat aaatagcatt tttagagacg gtatacaaac tcccatattt tctttagcta
     9781 gtgataatac tatatatatt tttagtacat ctaaattttt atgtgctggt cttagagtag
     9841 catttgtagt tgctccaaaa cgatatatag aaaatttaga aaatgcacta tataatatga
     9901 atctaatggt ttctccattt acagcagaaa tagtacatcg tttattatat tctcctatta
     9961 tagataaaat tatagaagag aaaagagatg caattataga aagaaatgaa gttgctgata
    10021 aagttttgtc agattataat ttgattggtg ataagaattg cagttttcgt tggttacttc
    10081 ttcctgatga attggatgga aaatcatttg aaatttctgc aaaaaatcta ggagttcaag
    10141 tatattgtgc tgaacgattt tcagtaggaa actctacagt tccaaaagca gttcgtattt
    10201 gtgtaacagc tccaaaagat gtagaagaat tagaaaaagg gcttaatata ataaagtcac
    10261 ttcttaagag ttaaatttat tttactatta tttaattaaa taaataaaaa ggcggagaaa
    10321 tttattataa taaacctccg
//
LOCUS       DACTGW010000140        10303 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.140, whole genome shotgun sequence.
ACCESSION   DACTGW010000140 DACTGW010000000
VERSION     DACTGW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.140"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..1685)
                     /locus_tag="I9573_002584"
     CDS             complement(<1..1685)
                     /locus_tag="I9573_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAT4797194.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASSFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGTNINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFD"
     gene            complement(1708..2964)
                     /locus_tag="I9573_002585"
     CDS             complement(1708..2964)
                     /locus_tag="I9573_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAT4797195.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYKVSKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIEGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTTVIISDILCVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            complement(3013..4677)
                     /locus_tag="I9573_002586"
     CDS             complement(3013..4677)
                     /locus_tag="I9573_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HAT4797196.1"
                     /translation="MMFKIFNKSLLKLVIVLSIVLTISNFSLFSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINENYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKVTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKYISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            complement(4667..5515)
                     /locus_tag="I9573_002587"
     CDS             complement(4667..5515)
                     /locus_tag="I9573_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT4797197.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            complement(5959..7011)
                     /locus_tag="I9573_002588"
     CDS             complement(5959..7011)
                     /locus_tag="I9573_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HAT4797198.1"
                     /translation="MIKFKKTISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            complement(7011..7796)
                     /locus_tag="I9573_002589"
     CDS             complement(7011..7796)
                     /locus_tag="I9573_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002846538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797199.1"
                     /translation="MLKLKKITSNIYLALVFLFLYAPIFALVLFSFNDSKSMARWNGF
                     TWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGTISAIGIHKMRGKSKKLILNVN
                     YLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIMFCLPYVILSVLPKMKQLPDNI
                     EDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTMSIDDFIISFFNAGNGVSNLSI
                     EIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKESIVRGIK"
     gene            complement(7790..8626)
                     /locus_tag="I9573_002590"
     CDS             complement(7790..8626)
                     /locus_tag="I9573_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797200.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            complement(8628..9671)
                     /locus_tag="I9573_002591"
     CDS             complement(8628..9671)
                     /locus_tag="I9573_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797201.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            complement(9690..10229)
                     /locus_tag="I9573_002592"
     CDS             complement(9690..10229)
                     /locus_tag="I9573_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAT4797202.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
BASE COUNT         3404 a         1749 c         1004 g         4146 t
ORIGIN      
        1 ttatcgaact ttatatttaa attattattt aacatcttta taacagaatt attatgtggc
       61 actccaaaaa ctacaatatt agtatcctta tacttgtcac taaattcttt tcctctaatt
      121 acatttatat ttccattatg tgaatttata ttcgtaccta aagtaacacc taatctaaac
      181 atccaggtca tagcttgtga ccctgaataa tctggcatta tcacagttaa atcattaaac
      241 tcatcatctc taacaaatgg atacggataa ttttcaaaag ataaggtttc attttctttc
      301 gtagataacg ccaaataaga attatttgat acatatgccc aaggattatt actctctcta
      361 gtaacacaat tcgaattttt tatactcaaa ttaaatgtca atttaacttg ataatagttt
      421 ttattatcta tatctttagg tatttttaat tctaacttat cattattaga atgtgaccta
      481 tccaattttt tacttcccac aataacatca tttatagaca ctgtaactaa agatttttca
      541 aaatctaaat tgtcagaata tcttaaattt aagataatct ttgaaccatc atctaatacc
      601 ttacccgttg gtattttgac atcaaacaaa gcttgttgat taaatgctcc ttccaacaaa
      661 aagtcagaat atcctaagtc ttttaatgtt aaatgaccta aatttaattt ttgttcccta
      721 ttaattttta tattagtctc tttgtttaca agcactgatg atgataaaac ttggttagaa
      781 agtctatttt ctattaataa atttgatgcc tttattaaat catcttcatt actaccaatt
      841 accaccatca tcttcttatt cttattgtat ggtgaatcta cttgcttgat tatacagttt
      901 gaagataata gggtttgttc ctttatactt aaaatatcca aaatttcttc tgctgtattt
      961 tcaggtttac caatatatat aatattatca tctgaccaat ttttcatttc tgaatataat
     1021 ttaacatcta actttaaatc atcatttttt gttatttttc caaatgatga tgccaaatta
     1081 aacactgctg tagtctctcc tctagtcata ttatctggta ctactatagt tgtatctaat
     1141 ttatgattat tttcaatttc tgcatatgga tacggatatt catttattga gttttccacc
     1201 tttttctgct tatatctaat tgatatatat gattctttgt gaataaccat ccagttccca
     1261 gtattggaat catcttggca aattttatca gatattgttt tgtatgtttt tatttttatt
     1321 tcattatatc cttgaattat ataatctttt ggaactaata cttctaatgt atttttataa
     1381 tttgtcttag catttaattt aatagatttt attggtacat tatttatcaa tacagttaaa
     1441 cttgaaacat ctccatttaa tatttggctc ttactaaaat taagatgcaa taaaacttct
     1501 tcaatatccc aatttttatt tacttcaaaa aaagtactat tacttcctat aacaccatca
     1561 atagttatat ctctttcaaa cttataattt ttaactttag tctcatcagc atttactttg
     1621 tatataagtg atatatttga aaaaaacaat actaaagata taattgatat aataaatttt
     1681 ttcatgttaa ttcctcctgt acatgaatca aaatctttct gttttatacc atttaacctc
     1741 tcttttaaaa attatatctt ttagatactc tatcatccct tttatagcta caaacagcca
     1801 tagttgagag tatgtaaaat acattattgc aactatgaat atattttcaa ttgttgcttc
     1861 acctttttct atagtaaggc ttatactcac ttctataata aataaaagat aagatagtat
     1921 ccatattaga aaaaagttaa ttggaataga aatttctact agtttagata tacttaaaac
     1981 acataaaata tcggatataa ttacagttgt taaaaataga aaatatacag aaaaaaagta
     2041 agctatatca aacacaatct tatttcttcc ctgtttaaat atatttttta tatatttcat
     2101 taatacatat atattccctt ttgcccatct agtacgttgc tttatccaca ccttaacagt
     2161 ttctggctct tgttcccatg ttatagattg tggtacaagc tttatcttgt atcccaattt
     2221 atatatccta aaacttattt ctgtatcttc tgcaattgct ttactatccc atccttcaat
     2281 ttcttctatg atacttctcc tcaatataaa gttagttcct ggtattgtac atagattaaa
     2341 aagttgccat ctaccagctt gtgacatcca ttgaaagctt aatgtttcta tgtttataaa
     2401 tttggttaat aggtttttat ttttatttcg agtcctaaat ttcccaataa cagcacctaa
     2461 ttcatcattc attactatag tttgtactag atatctaagt gcatttttat ctggtgtgtt
     2521 atctgcatca taaacagcta taaaatctcc ttttgaaacc ttatatccaa tatttaatgc
     2581 attagacttt ccttttccac ctgttaaact atctgtattt attatagtga aattataatt
     2641 gttgtaccta tcctttatat tttctaaaat ttctttacta ttatcagaag aattgtcatt
     2701 tataacaata agttccatct tactcttagg ataattgagt aataacaaag actccacagt
     2761 tctacctatc actttagctt cattatgagc aggaacaagt attgatatca taggatattc
     2821 atcaatttct tttatttctt tatcaaagtt tttaaaataa aatatatatc cacccatagc
     2881 taaaataata tttataagca ataaagacca tatactaaac agactaaata cgaacaaata
     2941 ctctgataca tttaaatctc tcataaattt ctcctaaaat attttaaaat attgattcta
     3001 atataaaatc atttatttta aaaatttctt tttatctatt ctcttaaaat atataaatat
     3061 aatcacaaat attaaaacac ttatagatac tatgaatatt aaaattttac taatattgga
     3121 tatgtattta aatctagtat cacttttagt tatttcatct aagtccttat catagttgac
     3181 tataatttca tctccattat ttcttatttg tatttcatca acttttaccc aattattaaa
     3241 atctcttaaa ttcataaatt ctatattctg tttttctaaa tattctattg tgttttttag
     3301 atactctatg tttaaatatg aatgaaagaa gaaccctcca ctaaaacctc ttacaataga
     3361 taatttatct aaattttctt ttatattttg aaaagtaaac ttgtcttctg ggtctatata
     3421 tcctaaattt tcgggtataa atatattaaa ctcctctgta tccctaataa tataaggata
     3481 tgtatttgtg ctaaattttt tatcattatt ttgatgttgc cccatataag tagaaaaata
     3541 tttttttaat tctttatatc cctctgattc cattgcataa tgtggcgctt caaatgcaag
     3601 aggataaatt ccattctcta tacatactct aagaccattc aaaactctat ctttgacaaa
     3661 tatcttcata ttttctttta agggttcatc ttttttacca tcccaaaatt catacccttc
     3721 tccagaaact tcttctttct tgtattgatg agtatatcca tgcaatatca cagttccacc
     3781 tttatcttgc atatatttta ttgctttgac aatttcaggg ctttctgata aagttattac
     3841 tttatgattt ttaggattta catatgctgg tattaatgca atcgtaaatg gtatattctt
     3901 actacttaaa taatccgcta tctctacaag attttttggt tctctaaaag catgtacatc
     3961 ttctattctt acaaatatac gtcctttatc aaacgtctta atattaaaaa tatcattaag
     4021 tgaatcgcaa aatatataaa ataaaactcc atctaaatct aattttgata cataaaaaag
     4081 atttttatca tttattatat atggatactt atttaaggta tcattaatac ttcctattac
     4141 tttattactt gtatcttttg attctacaat gttaaaaaca taatgttcat ccaagttata
     4201 tgatttacct ctataattta cactaactat attgtttgtt tttcctacat agtctaaatt
     4261 gtatttttta tgttccaaaa gattttctat accatatcct aaccaacata tagttacttt
     4321 atatgaattt aaggaactaa tcaagttttt agtgttttta tcattattat atgagttttc
     4381 attgattcct aaaataaata tatgactgta ataattttca tttatctcac catcatagtt
     4441 actcaatttt aatagctcta tatctgaact aaatcttccc aatagagttc tcataatatt
     4501 taaaatatct ctattataag ctgtttcttt cttagaatca tatatgatta ataccttatc
     4561 tgtgctggaa gtaatttctg atgcatatga aaataagcta aaatttgata ttgtcagtac
     4621 tattgataat acaattacta attttaataa acttttatta aatattttaa acatcatatt
     4681 gaagttcctc ttcaactata ttcttaaaat ttatactatc tccaaaacta ctcttatatt
     4741 gtaaaaatgc aattttgaca tctatattta catattttcc cctattgttt aaatctaaat
     4801 ttaattcttt tatccttttc tttattctgt ctttaacaac tttcgaacct tccaaagatg
     4861 tatttggcat tagtatccct atcatatctt tttgtaaact ataaattata tcttcatttc
     4921 ttgtacattc tataatattt gaaccaatat attttactat tttattagtt ttgttttcac
     4981 caaatatagt ctgtaggttt ccgtagtatg gtaattttac tatcataaga gaaaaatcag
     5041 tattatgtct aatagattta cttatctcca tatttacatc attataaaaa atctttaaat
     5101 ttctaagtcc agtttcacta tcaatagtca ctaattcctt atactgttca gatagttttt
     5161 tatttgtatt ttgcaattca tttatgcttt tgttcagatt acccattata attgaagata
     5221 ctggtgttgc tattatccac atatatgata taaattcaac atccatattg tgagtaatat
     5281 tgttatataa aatataggat gtatacataa atataattat tgaacttgtt atcaacccca
     5341 ctactgaatt tgataaataa gttaacatta ttataaaaaa aattattgct aacattatga
     5401 acacattcaa tttattttct gtcctaatcg atagtattac tattccaaca attaaaaata
     5461 taaaaacgag caaagccaat attaataaat ctattttttt atttatccta ttcatttttt
     5521 ataaatttac acctccaatt attctattaa gttgcccatt ttttctaatt attattaaaa
     5581 tcattataca aataagtttt catttattaa taaactattt aaattgcata tgaatataac
     5641 ttaataaaaa atactgtctc aaagcaaatt ctttagctca tgaatcactc tttttttatg
     5701 aaatcaaaca tgttttcatc atttcaacaa tgaaaaagag gcttatctca atttcgagac
     5761 aacctcttta tagttattat tataaaaata gtaatcaatg aaacttactg tactttaaat
     5821 tctcataaaa ataaactcaa aataaaaaca tgacctaaac aaaaaataaa actgttcaat
     5881 aattagaaaa taaattttct aaaaaagaac agttttatta agtatttatt aagtaaattt
     5941 acttaaacca acaagaccct attttgattt taactctatc caagccttgt catataattt
     6001 tatattttca ccaatatctt caaaagtttc acatttatca agtacttctt tagatggata
     6061 tgcaacagga ttctgagtta tttctgggtc taataatttt agtgctcctg tattaggtgt
     6121 tgaataccca atgtactcag catttacttt tgcattctct gggtctaata aaaagtttat
     6181 aaacagctca gcttctttct tattttgtgc tgttgtaggt acacacatag catctaccca
     6241 tttattagtt ccctctttag gtatagcata atcaaggtca ggattttgct ccattagcgt
     6301 aactgcatct ccagaataca gcatacccat tgccacttct ccaccaagca atctatcctt
     6361 accttcatca tttacataag ccaatactaa atccttttgt ttcattaaaa gatttttagc
     6421 ttcatttatt tcttttggat ttacactatt catgctatat cctaattttt taagagttat
     6481 ccctatagta tctctaacag agtcaaacat cattatattt cccttatact ttggattcca
     6541 aagtatattc cagctatcca tagggtcttt aacttctttc ttattgtaca atattccaag
     6601 agttccccac atatatgcaa cactatattc attagttttg tcgtatacag gatttaaaaa
     6661 actcttatct aaatatttca tattaggtat attcttaaaa tctaattttt ccaaaagacc
     6721 ttcatttttc attttctcta ccatatagtc tgaaggaaat actaaatcat agtgtgtact
     6781 accactcttt actttttgat acatcatttc attagtatca taagttgaat attgaacatc
     6841 tattcctgtt tctttttcaa acttatctat aagagactca tctatataat ctccaacatt
     6901 atatacattt aaaacttttg tagaatcagt tgctttatta catcctgtaa ataaagtaac
     6961 tgtcatcata cctacagcta taagggatat tgttttttta aacttaatca ttatttaatc
     7021 ccccttacta tagattcttt tttattagct agtaaaagta aacctagaac cactgcaaac
     7081 attattgttg ataatgcatt tatttcaggt tttatacctc ttctagccat tccatagatt
     7141 tctatagaaa gattgcttac accatttcca gcattaaaga aacttattat aaagtcatct
     7201 attgacatag taaatgcaat taaaaatcca gaaactatac ctggttttat ttgtggtaaa
     7261 attacttttc taagagcgta tataggtgtt gctcccaagt ccatagctgc atcttctata
     7321 ttatcaggta attgcttcat ttttggcaaa actgaaagta taacgtatgg caaacaaaac
     7381 attatatgtg ccaatagcat tgttgtaaat ccaaattcca tttttacaaa tacaaacagg
     7441 ctcataagag ctactgctgt aactatctct gtatttaaaa taggaagata gtttacattt
     7501 agtattaatt tcttactctt acctctcatc ttatgtattc ctatagcact tatagttcct
     7561 actattgtag atattacaga cgctaatata gctattacaa tagtataata taaagcactc
     7621 attatactct cattttgaag taattgacca taccacttcc atgtaaatcc attccatctt
     7681 gccattgatt ttgaatcatt gaaagaaaat agtaccaaag caaatatggg tgcatacaaa
     7741 aataagaata ctaatgctaa atatatatta gaagttattt tctttaattt taaaatagta
     7801 acccaccgcc ttctttgtca gattcatctc caaattttga cattatagac atagatatta
     7861 atattactat catcataaat atcgatatag ctgaaccaaa gttccaatct ccaacaactg
     7921 taaactgttg ttcaatcaaa tctccaacca gcattatttt acctccacct aacagtcttg
     7981 atattgcaaa agtagtgact gctggcataa ataccatagt tattccagac attacacctg
     8041 gcaaactcaa aggaaatata atttttctaa aaactgtcat attatttgca cccaagtcat
     8101 gagcagcgtt tatcaaatca ttatccatct ttgatagtgc tgtatatatt ggaagaacca
     8161 taaatggtaa aaagttatac accataccta aaagtatagc aaaattagta tacaaaattg
     8221 ctaatggttg tattccaaac caacctaaaa atgtatttaa aagtccattt tttcctaata
     8281 tagcaaccca tgcatatgtt cttaaaagaa agttcatcca cataggaaga ataaacaata
     8341 atattaatga acctcttcta cttacacgag cctttgaaat tatatatgcc actggataac
     8401 caactataag gcatattaat gtagctcctc ctgctaaaag aatagaacgc ccaaaaactt
     8461 tcatatatat agggtcaatt acttctttat aattctcaag tgtaaaggca taacctccac
     8521 ctgattcttt tgtaaaacta aaaaatacca ctaatattaa aggaattatt acaaatatcg
     8581 cactccaaac tacatatgga tatgctaaaa aagattttct tttcatctta gttattctct
     8641 tttctcatta tatggatatc ttctggatat atatccatgc ctagttcagt cccaacctca
     8701 gcatttttgg tattatgaag tatccacaat cttccatttt cattcaattc tatctcgtaa
     8761 tgcactcctt taaacactgt agatgtaact ttccccttta acattccttc ttctggcttt
     8821 accattttta tatcttctgg tcttattact acttctatat tttcattttt ttcgaatcct
     8881 ttatcaacac actcaaaatc tctattacag aaattaactt tataatcttc tatcataatt
     8941 ccatcaaata tgttactttc acctataaat ttagctacaa atgaattttt aggttcattg
     9001 tatatatctt gtggtgttcc catttgttga attttgccct tatacattac aacaatagta
     9061 tctgacatag taagagcctc ttcttggtca tgagttacaa aaacaaatgt tattcctaat
     9121 tttttttgga ttctctttaa ttctatttgc atctcttgtc ttaactttaa atcaagtgct
     9181 ccaagtggtt catctagtaa taataccttt ggttcattga ctagagctct tgctattgca
     9241 attctctgtt gttgtcctcc actcaatgat tcaacttgtc gtctttcaaa accttctagt
     9301 gcaacaagtt tcaaaacctc ttttacttta gcatctatta catcttttgg agtcttttta
     9361 atttttaatc caaaagctac attttcataa acatccatgt gtggaaataa agcatatttt
     9421 tgaaatactg tgtttacttg tctttcatat ggtggcaagt tatttatctc cttaccttca
     9481 aataaaactt taccatcatc agcatactca aatcctgcta ttatttttaa tgttgtagtt
     9541 tttccacatc cacttggtcc aagtaatgtt aaaaactcat ttttctttat atctaaattg
     9601 aaattatcta aaacagtaag ttcctcataa tgtttagata tatcccttaa ttctattata
     9661 ttttctacca attattaaac acctctattc taaaatgatg gtggcgtact tacccaaatc
     9721 acttttgctg tagttttgcc ttcgtttgat atatagtgat ttgcttttgg tctaaagtaa
     9781 aaactctctc ctttttttac tttatttttc ttttcgccta tatggacata tattgaacct
     9841 gctaatacat atccaaattc ttcaccttca tgtggctttt cttctttata ctgtcctcct
     9901 ggatacaatg ttattattat tggctccatt tcattttttt gtgaatttgg aattaaccac
     9961 tttaatttat attttaaatc ttcatcttca gtttcaaaca tatcttcttt tgtaaaactg
    10021 attctttcat catctatttc attaaaaaat tcccttaaat ttgtacctag tatttcaagt
    10081 atatctataa gggtagctat ggatggtgat gttaagttat tttctaattg agaaataaat
    10141 ccctttgaca attcacatct attagctagc tcttcctggg tcagttgttt ttctgttctt
    10201 aatcttttta tcttttcacc tatatccatg ttgcacctcc tagtttctgt tttattttac
    10261 taaacatttt gtttactaaa cattgagttt aaaatttcta aac
//
LOCUS       DACTGW010000141        10273 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.141, whole genome shotgun sequence.
ACCESSION   DACTGW010000141 DACTGW010000000
VERSION     DACTGW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.141"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            2..1843
                     /locus_tag="I9573_002593"
     CDS             2..1843
                     /locus_tag="I9573_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAT4797203.1"
                     /translation="MKGTIQNSLTKEAILHIPMSNYAYGYDRETLHIRLRTKKNEAKR
                     VILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETELFDYWIAKCKPLNKR
                     SRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPYLNYIDIPKVPNWVK
                     DTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGVIEHLDYLSDLGING
                     IYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRNIKIMLDAVFNHIGY
                     YSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYETFGCEKYMPKLNTE
                     NSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEVKKVKPDIYILGEIW
                     HGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMINDVMVSYPIQVSEVIF
                     NLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEIGMEGELSSISEGQR
                     KCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKENGTIILKKESISII
                     INNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFIILK"
     gene            2067..2747
                     /gene="pgmB"
                     /locus_tag="I9573_002594"
     CDS             2067..2747
                     /gene="pgmB"
                     /locus_tag="I9573_002594"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAT4797204.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2744..5029
                     /locus_tag="I9573_002595"
     CDS             2744..5029
                     /locus_tag="I9573_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAT4797205.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNKVASIEKYVSITSTRDYKESELLR
                     KCEDILNIELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNCIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKYIKIDK"
     gene            5255..6148
                     /locus_tag="I9573_002596"
     CDS             5255..6148
                     /locus_tag="I9573_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase"
                     /protein_id="HAT4797206.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIAEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEV"
     gene            6196..8496
                     /locus_tag="I9573_002597"
     CDS             6196..8496
                     /locus_tag="I9573_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797207.1"
                     /translation="MKKIFSIFISCMVVVTTITGCSSGEVIKNTSPINSVKNLDFSFE
                     VKPEIFEVSVESGGSKEKISEPSKERKVSNFKKNKTEASWTYPDENIDINIKKEDSYL
                     DVNIKSNLEKETSFNWPKVEGESYILPLYEGKFIPSNDKYWKEYLNESTYNVIESFSM
                     QFFAVNKAKYSVIYIIENEFNNVIEFSSKDKINFTFKHEYPSINKNKEYGFKIYLTNK
                     NITDVAKTYKNYIVDKGEFKTLKEKSKENKNIEKLYGAPHIYFGDKSIITINDIEWNK
                     LKENLSDDLTNWMKELLSMKSGNSEFIKVINQIKTQDYIDKYQKNQIVQAFNEVMQMN
                     TFYNEKVFESKDREIKKVLDKGISKLSRVELIEFNKKLLKSKLEESVNDVATWAKYNT
                     TDVINDINKSGIKNAWIGFDDMEEGYISLEMVKRANELGYLVGPYDSYHSIHKPGEEK
                     WSTASFKDKSLYENATIENKKGEKETGFQGEGRKLNPTLSLPSVKERVQSILDEGYEF
                     NSWFIDCDATGEIYDDYSQNHITTQTQDLNARLERMSYIRDHANMVIGSEGGNDFSSS
                     TIAFAHGIETPAFAWVDKDMSKNKGSEYYVGKYYSPTGGVPEIFSKQIPVKERYKKIF
                     IDPIYSLPLFRLVYNDSVISSHQWLWGTLKIKDEVGSRMMKEVLYNTPPMYYIDRTEW
                     EKHKDEIISNNKVWSAFNKKAIKKEMTDFKFISEDRLVQMTEYGEDLKVLVNFSQKDI
                     KYNDEVIKANSLVIYDADSKVIYKPE"
     gene            8730..9221
                     /locus_tag="I9573_002598"
     CDS             8730..9221
                     /locus_tag="I9573_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HAT4797208.1"
                     /translation="MYENNKVIYNFERCEKLENALYLSQFNTYKNFKLTSKKTVINSL
                     DTSTYNMNIHSISLVDTMECISSEDEQMTGKKLILVGDIDTDVTLDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            9249..10196
                     /locus_tag="I9573_002599"
     CDS             9249..10196
                     /locus_tag="I9573_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797209.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNSIVFGCIEVEIDYSCKNR
                     SNFKTVNKFNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         4327 a          924 c         1798 g         3224 t
ORIGIN      
        1 aatcaaagga acaattcaaa atagtttaac aaaagaagct atattacata taccaatgag
       61 caattatgcc tatggttatg atagagaaac actacatata agacttagaa ctaaaaaaaa
      121 tgaagcgaaa agagtaattt tacgaatagg agaccaatat gtgtgggata aaggaggtgc
      181 aggaggagga aatttaaatg catctggagt tggttggtct ggtggaacaa atatagtaat
      241 gagtaaagaa gtagaaactg aattatttga ctattggata gctaagtgta agcctttaaa
      301 taaacgctca cgatatggtt ttataataga aggtcaagaa gaaaaacttt tatttacaga
      361 aaaaagaata atagagttgg gaaatgaaaa tgatgaaaag gaattatgtg aaataggtaa
      421 tttttttgga tatccatatt taaattatat agatatacct aaagtgccaa attgggtgaa
      481 agatactgtt tggtatcaga tatttccaga tagatttgca aatggtgacc cgtctataaa
      541 cccagaagga gtagataaat ggggagctat tccaaccaga gataatttta caggtggaga
      601 tttacaaggt gtgatagaac acttagatta tttgagtgat ttgggaataa acggaattta
      661 cttttgccct attacaattg gaaaaaccaa tcatagatat gatacggtag attatatgga
      721 ggtagaccct acattaggag ataaagagac tttaaaaaaa ctaatagaag aagcgcataa
      781 gagaaatata aaaataatgt tggatgctgt ttttaatcat ataggatatt attctaaaca
      841 gtggcaagat gtagttcaaa ataaggaaaa ttctcgatat aaaaactggt tttatataaa
      901 agatatgagt aaaattgata ctccaataga acagatagat gaaaaaaata taccttatga
      961 aacctttggg tgtgaaaaat atatgcctaa acttaataca gaaaattcag aagtaataaa
     1021 atatttattg gatgtaggca agtactggat acaggaattt gatatagatg cgtggagatt
     1081 agatgtttca aatgaagtag accatgtttt ttggagaaag tttagagaag aagtcaaaaa
     1141 agtaaaacca gacatttata tattgggaga aatttggcat ggtagtttac catggctaat
     1201 gggtgaccaa tttgattcag ttatgaatta cttgatgtca gaagccatga aaaaattctt
     1261 ttgtacagat gagataaacg ccgaagaatt taagtatatg ataaatgatg taatggtaag
     1321 ttatccaata caagttagtg aagttatttt taatttactt ggaagtcacg atactacaag
     1381 aatattgact tatgctaatg gaaatattga tagatttaaa cttgcatata tgtttatgtt
     1441 tgtacaaaca ggatgtcctt gtatatacta tggtgatgaa atcggaatgg aaggagaatt
     1501 aagttcaata tctgaaggtc aaagaaaatg tatggaatgg aatgaagaaa aatggaataa
     1561 ggatatattt gattttatga agaagattat tagacttcgt aaagaaaata aagaacttag
     1621 gtctatatca aatgaatggg tgttagcaga taaggaaaat ggaacgatta tattgaaaaa
     1681 agaatctatt tctataataa taaataattc atctaaaaat ataattttga cgttaccaga
     1741 ttatttagca aataaaaaag taaaagattt atatgaagaa gaaatagttg atttaaaaga
     1801 agaactaatg ttagagaaat acaagttcat tattttaaaa taatacaaaa agtatttatt
     1861 ttagatttca tgttaaatta ttatacagtt taaacaagtc tttataaaac ataaaaaaat
     1921 atggtttaca agtgagtgaa ttgaaagtag attttaaatt aaagactgag ttagagtaga
     1981 taattttata aataatatct taaatacaag atagaaaata aattgattat tagtctaaaa
     2041 atttcttgta gaaagaggaa ataagaatga ttgaagcatt tatatttgat ttagatggtg
     2101 taattacaga tactgcatat tatcactata tggcttggag aaaactagcc cataaagttg
     2161 ggatagatat agacacaaaa tttaatgaat ctttaaaagg cataagtaga atggagtcat
     2221 tggacaggat attagaattt ggaaataaga aatattcatt ttcagaagaa gaaaaagtga
     2281 gaatggctga agaaaaaaat aattactatg tatcattgat agatgagata acttcaaatg
     2341 atattttgcc aggcatagaa agcttgttaa ttgatgtaaa atctaataat ataaagatag
     2401 gtctatcttc tgcaagtaag aatgcaataa atgtattaaa tcatttgggt ataagtgaca
     2461 aatttgattt tatagctgat gcaggaaaat gtaaaaacaa taaaccacat ccagagatat
     2521 ttcttatgtc agcgaaaggg cttaatgtaa atccacaaaa ttgtataggg atagaggatg
     2581 caagtgcagg tatagacgct ataaactcag ctaatatgtt ttctgttggt gttggaaatt
     2641 atgaaaactt aaaaaaagca aatttagttg tagattcaac aaatcaatta aagtttgaat
     2701 atatacaaga aaagtataat gagtatatag ttaggagaat tatatgaaat gtttatttag
     2761 taatgtagat gagtggaaga ttatacaaga taagattgaa atcaaagaaa atagattagc
     2821 agagtccata atgagtatag gcaatggata tatgggtatg agaggaaact atgaggaaaa
     2881 ttatagtgga gatacacaca aggggtctta tatagctgga gtatggtttc cagataaaac
     2941 tcgtgttggt tggtggaaaa atgggtatcc agaatacttt ggtaaaattc ctaattcagt
     3001 caactatata ggtgtaagaa ttttcataga tgatgtagaa ttagatttag caaaatgtaa
     3061 agtagaagat ttttatagag aattagatat gaaaaatggt attttaaatc gtaggtttat
     3121 tgtaaatata aatgataaaa aatttgaagt tagggttact aggtttttaa gtatatctgt
     3181 taaggaactt gcagtaatta aatatagtat aaaatcatta aattctaata ctaaaataaa
     3241 atttgtacca tatttagatt caaatataaa aaatgaagat tcaaactatg aggaaaagtt
     3301 ttggaatgga atttcttcaa gttctttaga atataaaggt agtattcttt ctcgaacaaa
     3361 ggagaatgca tttaatacac cagtattcac tgtgggttca atgatgtata ttaagacaaa
     3421 tggtgatgta gaagctgtat ctcatgagag tgattataat tactgtgaaa attcgataac
     3481 aatggatgtt ttaaaaaata aggttgcaag tatagaaaaa tatgtatcta taacttcaac
     3541 tagagattat aaggaaagtg agctattaag aaaatgtgaa gacattttaa atatagaact
     3601 atctaaaagt tatgatgata tattaaatga acatacaaag ttatggaata aaagatggga
     3661 aagtgcagat gtaaaaataa atggagataa aagtagccaa caaggtataa gatataattt
     3721 attccaacta ttttcaacat attatggaga agattcgcga ttaaatatag gtccaaaagg
     3781 atttactggt gaaaagtatg gaggagctac ttattgggat acagaagcat tttgtgttcc
     3841 agtatatcta ggtgtagcag ataaggaggt aactagaaat cttttagaat ataggtacaa
     3901 tcagttagaa caggcaaagg ataatgcaaa aaaactaggg cttaaaggtg ctttatatcc
     3961 aatggtaaca tttgatggag tagaatgtca taatgaatgg gagataacat ttgaggaaat
     4021 ccaccgaaat ggaagtatag tatatgcaat atataactat acaaattata caggtgacta
     4081 taaatatata aaagaaaaag gaatagatgt aataatagaa atagctagat tttgggcaag
     4141 tagagttcat ttgtctacaa aaaaagattt gtatatgata catggagtaa caggtccaaa
     4201 cgagtatgaa aacaatatca ataataattg gtatactaat tgtatagcta aatggtgttt
     4261 agattatgct gtagaaaata tcttacgatt agagaaagag tgtaatgaat gtattgacag
     4321 aaataatgtg actagggaag aagttgagtt ttggaaaata atatcaaata aaatgtatct
     4381 gccgtatgat gaggaactac aaataataat tcaaaatgat aattttttag ataaagaatt
     4441 tgtagaagta aaggatttac caaaagaaaa ttttcctctt aatcaaaatt ggtcttggga
     4501 taagattctt aggtcgtgtt ttataaaaca agcagatgta ttacagggaa tttactattt
     4561 tggaaataga tttactaaag aagaaaaaaa gagaaatttt gacttctatg aaaaatatac
     4621 tgtccatgaa tcttctttat cttctagtat ttattctatt attgcctcag aaatagataa
     4681 cctagaaaag gcttatgaat tatactcaag aacagctagg cttgatttag ataattacaa
     4741 taatgataca aatgatggac tgcacataac ttcaatgtct ggttcatggc tttccattgt
     4801 acatggattt gcaggcatga gaacatggaa tgaaactcta tcttttgacc ctaagttgcc
     4861 aaaagagtgg aaaaattatt ctttcaatat aaattataga gataatatta ttaatgtaga
     4921 tgtatgtagt gatttaatta aaatagaaaa tttaaaaggt aaaccaataa aaattattgt
     4981 atatgggaaa aaatatatat tagaaaaata tataaaaatt gataagtaga tttaataata
     5041 tttttattat ataaatagtt aaaataagtt tatttaatta tttatctatt ttaactattt
     5101 tgtgtgatag aaaactatat attataacaa tagtagtgcc tttaattaaa tatatttcat
     5161 aaatcttcat aaaatcttca aaatttcgta aagaatatgt cacaaaagta aggtatatta
     5221 tattcagcag atagtataag ggaggacaat atttatgaag aataaaaaat tcattgttat
     5281 aatactgata atatcaattt taataggtgt attagtgaag gaatattcat ctagagagat
     5341 aaaaaaggat gatataaatg tcagtaagta tataaaatat gctgatttag caagtaaaaa
     5401 taatgcacaa gttaactgga agtatgtagc ttccattgtt gctgtgttaa ataaaaataa
     5461 cttaaaaaac gttaaggatt cacagataca agaagtatca gatttatttg ttaagaattt
     5521 ttctaaaaat aataaaataa ataagttaag tgatattttg gatgagttag aatttagtaa
     5581 tagacaaaaa agattggttg ataattatat agacaatctt aaggattatg gtataaaacc
     5641 agaaagattg aagtctgata caaaatatat gaagtttatt gctgaaatta aaacagaagc
     5701 aatacaaaat tataaggatt ataaaatact tccatctata actattgcac aagctataat
     5761 agaatctagc tggggcaagt caactttggc aaaacaatat aacaatctat ttggtataaa
     5821 ggctgacgct tattggaaag gtaagtcagt gactttagaa acgaaagaac atttagatac
     5881 aattatagat gataaattta gaatatatga tgataaaaat gaatctataa aagaccatgc
     5941 aaaattttta gccacaaata aaaggtataa aaacaatggt gtatttgatg caaaaacgta
     6001 tatatatcaa gctaaagcat tagaaaaggc aggttatagt actgcgaagg atgaaaatgg
     6061 caatagtata tatgcagctc gtctaataga attaattcaa cagtataatc ttcaattaat
     6121 agacagcgaa attcaaagtg aagtgtaact tttatgtaac ataggagtga tatatttatc
     6181 gaggaggtgg tttttatgaa aaaaatattc tcgatattta taagttgtat ggttgttgta
     6241 actactatta ctggatgtag tagtggtgag gtgataaaaa atacatcacc tatcaatagt
     6301 gttaaaaatt tggattttag ttttgaggtg aaacctgaaa tatttgaggt atcagttgaa
     6361 tctggtggtt caaaagaaaa gatatctgaa ccctcaaaag aaaggaaagt atcaaatttt
     6421 aaaaagaata agacagaggc atcgtggacg tatcctgatg aaaatataga cataaatata
     6481 aaaaaagagg acagctattt agatgtaaat attaaatcta atttagaaaa agaaactagt
     6541 tttaattggc ccaaagttga aggggaaagt tatatactac cactttatga gggtaagttt
     6601 ataccaagta atgataagta ttggaaggag tatctaaatg agtcaactta taatgttata
     6661 gaatcttttt caatgcaatt ctttgcagta aacaaggcaa agtattctgt gatttatatt
     6721 atcgaaaatg aatttaacaa tgtaattgaa tttagttcta aagacaagat taattttaca
     6781 tttaaacatg aatatccaag tatcaataaa aataaggagt atgggtttaa aatatatttg
     6841 actaataaga atattacaga tgtagcaaaa acttataaaa attatatagt tgataaagga
     6901 gaatttaaaa cattaaaaga aaaatctaag gaaaataaaa atattgaaaa actttatgga
     6961 gcaccacata tttattttgg agataagtct atcatcacaa taaatgatat tgaatggaat
     7021 aaattaaaag aaaatttatc agatgatttg actaattgga tgaaagagct tttaagtatg
     7081 aagtctggca atagtgaatt tataaaagta attaatcaaa taaaaactca agattatata
     7141 gataaatatc aaaaaaatca aatagtacaa gcatttaatg aagttatgca gatgaatact
     7201 ttttataatg aaaaagtttt tgaaagcaaa gatagagaaa taaaaaaagt attagataaa
     7261 ggtatatcta aattaagtag ggtagaactt atagagttta ataagaaatt attgaaatca
     7321 aaattggaag agagcgtgaa tgatgttgct acatgggcaa aatataatac aactgatgta
     7381 attaatgata ttaataagtc tggtattaaa aatgcgtgga taggatttga tgatatggag
     7441 gaaggttata tatccttaga gatggttaaa agagctaatg agttaggtta tctcgtaggg
     7501 ccatatgatt cataccactc tatacacaaa ccaggggaag aaaagtggag tacagcatca
     7561 tttaaagata aatctctata tgaaaatgct accatagaaa ataaaaaagg tgaaaaagaa
     7621 actggatttc aaggagaagg aagaaaacta aatcctacat tgtctttacc aagtgtaaaa
     7681 gagagagtac aaagtatatt ggatgaagga tatgaattta attcttggtt tatagattgt
     7741 gatgctacag gagaaatcta tgatgattat tctcaaaatc atataacaac acaaacacaa
     7801 gatttaaatg ctagacttga aagaatgtca tatataagag accatgcaaa tatggtcata
     7861 ggttcagaag gtggaaatga tttttcaagt agtactatag catttgcaca tggtattgag
     7921 acaccagcat ttgcttgggt agataaagat atgagtaaga ataaaggcag tgaatattat
     7981 gttggtaaat actattctcc aacaggagga gtaccagaaa tattttcaaa gcagatacct
     8041 gtaaaagaaa ggtacaaaaa aatatttata gacccaattt actctctacc tttatttaga
     8101 ttagtttata atgattctgt gatatcttcc catcagtggt tatggggaac tttgaagatt
     8161 aaggatgaag ttggaagtag aatgatgaaa gaagtattat ataatacacc acctatgtat
     8221 tatatagata gaactgaatg ggaaaaacat aaagatgaaa taatctcaaa caataaagta
     8281 tggtcagcgt ttaataagaa agctattaaa aaagaaatga cagactttaa atttatttct
     8341 gaggatagac tagttcaaat gactgaatat ggagaagatt taaaggtatt agttaatttt
     8401 tctcaaaaag acattaagta caatgatgaa gtcataaaag caaattcttt ggtaatatat
     8461 gatgctgata gtaaagtcat atataaaccg gaataataga tttaatgaaa ataaagattg
     8521 tttaaatttc aagtaaaaaa taatataata ttcatttagt ttgtattaaa gtagtgacta
     8581 tattaatctg gataattagt atagtcacta ttatttatat tctttcctaa aagtaagtag
     8641 taactgaatc gtgaataatc aaaaatggta atttgaaata gtttttatga atagtattaa
     8701 tatatagata tagggagatt gatgtgatta tgtatgaaaa taataaggta atatataatt
     8761 ttgaaagatg tgaaaagcta gaaaatgctc tttatttatc tcaatttaat acatataaaa
     8821 actttaagtt aacttctaaa aaaactgtaa taaactcatt agatacatct acttataata
     8881 tgaatattca tagcataagt ctcgttgata ctatggaatg tatatcgtca gaagatgaac
     8941 aaatgacagg aaagaagttg attttagttg gcgatataga tactgatgta acattagatt
     9001 atgcaggcaa taaaagaaat agaaattcta gtaaaaaaaa tacatttaaa ttaaaaatac
     9061 cctttagtac atttatacaa atgcctagaa agatagaaaa taaagataaa attaatttaa
     9121 agtatcttat acaagatatt acttcatcta ttttagatga taatttattt attagtgtta
     9181 ctgcaattat aagttatgaa aatagttcca agatagagta atctttaaat tatagaaagg
     9241 tggatgcaat gagaataacg aaaactactt ttaatgatgt attttcaaat gatgcaaatg
     9301 gaagtattat aaaagtagaa aaatatgttg ataaaaaatg tgcatctgta tttgagattt
     9361 tgacctacac tattattgtt acaaatacaa gtaggtacaa aactggaaat atatttttca
     9421 aagattatat ttctaaatat atagagttta taaataatac tgtaaaagtt aatggcatta
     9481 taaaacgtgg attagaccca cagcaaggtt tttacatagg gagaatagat gcaagttgta
     9541 aaaaaataat atcttttaag agtgtagttt taccaaattc tgcacatagg attataaaga
     9601 atagtgctaa tatatactat tattataagt gtaatctaga taaattccca acaagaatat
     9661 caatagaaag taatagagtt tatactaatg taaataaaat tgtattcaaa caattgaata
     9721 taagtaatac tttaaaagct cctaaaaatc taagagatat attaaaagta aatgtaaata
     9781 gtaaaatatt tgatgttaaa cctatgaaaa atcttataaa caaaggtagt aatttgaaat
     9841 tatgtaactc gatagttttt gggtgtatag aagttgaaat agactatagt tgtaaaaata
     9901 ggagtaattt taaaacagta aataagttta atgatattaa aaaagataag tattataaaa
     9961 atgatataca aaataacaat gaaaatataa acgattgtaa aaagcaaata agttttggtg
    10021 ataataaggt aaaaaaaata acaaaaactt ttggattttc ttgtttcatt tgttctccag
    10081 ttggaattat ttatgaagat atgaaaaata taaatataaa aattgaacat acatcaataa
    10141 atgaattaaa tccagaagaa ttatttgtaa atacatcact tttattgtat tattaagtat
    10201 tttaaagcta aaacttaaac ttattaaaat agcatttggt taaaggaaaa tagatattga
    10261 tatatttata ata
//
LOCUS       DACTGW010000142         9917 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.142, whole genome shotgun sequence.
ACCESSION   DACTGW010000142 DACTGW010000000
VERSION     DACTGW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.142"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(92..2623)
                     /locus_tag="I9573_002600"
     CDS             complement(92..2623)
                     /locus_tag="I9573_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT4797210.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(2731..3348)
                     /locus_tag="I9573_002601"
     CDS             complement(2731..3348)
                     /locus_tag="I9573_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797211.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(3710..4594)
                     /locus_tag="I9573_002602"
     CDS             complement(3710..4594)
                     /locus_tag="I9573_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HAT4797212.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAVKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(4785..6035)
                     /locus_tag="I9573_002603"
     CDS             complement(4785..6035)
                     /locus_tag="I9573_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4797213.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFIYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDCKAI"
     gene            complement(6257..7021)
                     /locus_tag="I9573_002604"
     CDS             complement(6257..7021)
                     /locus_tag="I9573_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797214.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(7015..7740)
                     /locus_tag="I9573_002605"
     CDS             complement(7015..7740)
                     /locus_tag="I9573_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797215.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(7742..8110)
                     /locus_tag="I9573_002606"
     CDS             complement(7742..8110)
                     /locus_tag="I9573_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4797216.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(8286..9830)
                     /locus_tag="I9573_002607"
     CDS             complement(8286..9830)
                     /locus_tag="I9573_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HAT4797217.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQFISKYAIQIILIMAIICMMLIFYIIYDKLNFKIKLQKIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIDLNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         3350 a         1780 c          910 g         3877 t
ORIGIN      
        1 atataaaaat tcaaaaaagg agttgaattt aaaatttgat tagaaaaaaa tcaattttga
       61 gacaactcct ttttatttta atgtatttaa attatagtat atcagccttc ataatatcct
      121 caaaatcacc tttttcagat attaatctta tcaatttaac agtacagctt aaatcattta
      181 taagtatagc tccaactaat gaaccatctt tcataaataa ttttttatat attctttgac
      241 cttcatcttt tgatgaaatt tctttatcaa aattttgtat attaccaata ctaaatactt
      301 ttacattcat tgcatctaat gaaactggat atacttcact ttcaaatgtc aaattatctc
      361 cacttgcatt tgctcctgct atttttccca tttctactga agatggccat attgctaaac
      421 taatatttcc cacttgtgca acatctccac aagcatatat atctttaaca tttgtctgta
      481 atgtcttatc tacaacaata cctctatcaa tttttacaga tgtatcctga accatttgag
      541 tgtttgctct tacaccaatt gaaaatacta ccatatcaca gtcaacacta tctccatctt
      601 taaatacaac tttttgagct tttccatcac cttcaatacc atcaactgct acacccaatc
      661 ttacatctat tcctgtatct cttacacatt gttctaatat tttactaccc tctttatcaa
      721 gttgttttga tagtatgcta tccatggctt caactactac tacttctaat ccttttagtc
      781 taaattccca tgcagcttca agtcccaata aaccgccacc aatcactaca actttcttag
      841 atttttctag tgcttctttt acactatata aatcttttat atttctaaga gtaaacacat
      901 tttctaaatc atggccctta atcggtggta taaagtttct acttcctgta gctattacaa
      961 gcttatcaaa tttaatagct ccatcattca ctataatagt tttattcact tcatctaatt
     1021 tatctacatt cgttcctaat attactacta tattattttt atcataccaa tctttatctt
     1081 ccatgtagaa atctgaacca agttcttcat ttataccatc agaaagtgca ggtctatagt
     1141 aagtcaactc atcttcatta gaaatcatag ttatcttaca agttttattt ctttttctta
     1201 tagcatctgc tgcataaaaa ccagctgctc catttcctac tattacgaaa tattcatcag
     1261 tatcttttct aaaagtaact tcatcttctt caacttctat aaattggtct tccttagctc
     1321 cacatacagg gcatacacta ggagcactat caccttcaaa aacttcatta caaattacac
     1381 atttaaattt cttagttttc tttttacttc caaaagtaga atttaactta tctaggaatt
     1441 tacctgtata agaatgtaat ttatcaatct cttcatttga tggtttaaag ttaacagcaa
     1501 aaggttttac tgctgtcatt ctaagctgac ttattctctc catagcattt tctatacctt
     1561 ctccactcca tccatatgaa ccaaatactg aagcaacttt tccaccatga agaactgggt
     1621 ttaacgatat taatatatcc catataggct ttaacgcatc tcctaatatt gttggtgttc
     1681 caaataatac accttgtgat actgctattt ttgccaaaat atcttcttgc ttatgctcta
     1741 ttacatcaaa taaatttact ttatagttgc tatttttttc aatatatgct tttatagctt
     1801 ctgccataat ctttgtatat ccatgtgcag aaacataaca aattgtaact tctttttcta
     1861 actttatttg ttcctttaca ctccagttat aataaaggtc tataattttt ctaggatttt
     1921 cagttaatac tggaccatga cctggacata cagtatctat atctaaatct tttatctttt
     1981 ctatagcagt taccatagat ggtttaaatg gacccattat acaatcataa tagtatctta
     2041 aagcatccaa gtaatccttt tcttcatttt catcaagtgt atttactatc ttatcattac
     2101 tatagtgact accaaatgaa tcacatgtta caagagtttt atcctctttt atatatgtgt
     2161 atatagtatc tggccaatga agcataggta cagagaaaaa ttctaaagtt ttgtccccaa
     2221 ttgataatgt atctccatct gtaactgcaa catattcaaa gtctttgttt acgatttcct
     2281 taagatattt aattgctgtt tctgaagcta ctacttttgc atttttagct aaatccaaaa
     2341 gcttttcaac gcttcctgca tggtctggtt cagtatgact tacaacaata tagtctattt
     2401 tttcaaaatc tatatttaaa tcatttaatc tttcaatata attgtcaaaa tgcttatctt
     2461 taactgtctc aaacaaaact gttttttctg ttccttttaa tacatatgaa ttataagtag
     2521 ttccataagg tgtatacata attatgtcaa atactcttaa atcagggtca agtgaaccta
     2581 cccaatataa attttctttt atttttagtg atttcaatga cataccgtac tcctcctaaa
     2641 atagttattt agtctcttta taccctgctt gttatatttt aaacaaaatt tattcaaaaa
     2701 agaaattatt ttaaagatag tttaaatctt ctaatcttct ttttctacct tcaatacagt
     2761 tcccttttta gaatctactt ttatgtcata ttcattttta tctttatcta ttcttatttt
     2821 ataaaaagta acatctaaat ctttgtctaa actccattct ttaaccattc cattttgttc
     2881 ttttaatgca atttccatag cttgttgagg tgtaattatg tcatttaaat ctaatttttc
     2941 ttttctagca actccattta aatcttctga atctaacttt tctgttttat cttctaatac
     3001 atcttttgta ttagcatcaa ttttcatctt atattcatta ttatcatcta caccttccac
     3061 agtataaaca aatttattta aatctctctc aagacttaaa tctactatat cagcatcctt
     3121 gtatttatct ttaaatatgt ttattgcatc tgtgtatggc actttaaaat cttgttccat
     3181 taaattttca ttattttttt catcttttaa agcattattt gaagcatttg cctccttttt
     3241 tgtattatct tgtgcttgtc ctgaatcctt agttgggctc ccactacatg cagttaataa
     3301 acctgctata agtatactta ccactaataa atacgttttt ttacacataa aaatcctcct
     3361 taaatatatt aatttattct atattatgcc ctctaaaccc tttatttacc cttttatatt
     3421 aaaaattcct cctattttat caattttaaa actttatatt tactaaaatt aaagttacat
     3481 aaaaattata ctgtctcaaa atagaaacaa gcatcttttt cattgttgaa ataataaaaa
     3541 tatattttat ttcgtacaaa aagagtgcct catgaactaa aaagttcatt ttgagacact
     3601 cccattcttt tatattttta cttacaaaat aacagtactt attaattttt tacaaacctt
     3661 ataagtatat ctatattata ttcttaaatt taaacactat attaaaaaat taaattttgt
     3721 ttaattcttt tactgttaaa tccattttag gatatggaat cgtaattcct tcttcgtcaa
     3781 acctaacttt aacctcttct agtaaatcaa gataaattgt ccaataatct tctgtcttgc
     3841 accaaactct agttgcaaat ttaacagcat tatctccatg ttctactact cctataaatg
     3901 gttctggctc atttattatt aatttatgat tattaacaat atctttaagt actcttctaa
     3961 catgtagtat atcatcttca taccctacac taaaagtcaa atcaactcgt ctagtattct
     4021 ttgctgaata gtttattaga ctatcatttg atacagaacc attaggtata agtatctgtt
     4081 tattatctgg agtaaccaat tgtgtataaa aaagtcctat ttgttcaacc gttcctccat
     4141 ggcctgctgc ttctacgtaa tctcctactt taaaaggtct aatcaatagt ataataaatc
     4201 ctccagcaaa atttgataga ctaccttgta aagccaaacc tattgctacc cctgctgatg
     4261 ctatcacagc agcaaaactg gacaaactca ttccccaata ttccagaaca atgattataa
     4321 cggcaacttt aagtacactc aaaagaagag acttcaaaaa ccttcttaaa gttaaatcaa
     4381 tatctctttt actaagtact aacataacat ggtttacaaa tttttttatt attttaaatc
     4441 caatagaaag tataagtata cctattataa gttttactcc gcttgtagta gcccattcta
     4501 gcagactttc cattatacct cccatatcca tcttattaat gtctaaatta tttaaatcta
     4561 acttgcttat atcatctatt ttcgtcgtat ccataaaaaa ctccttacct tatattttag
     4621 aaccaatatt atagtttaaa gttcttattt tctatttaat ataatacaat accatttgta
     4681 catagtaaac aagatttata ttccaattat acatcttagg aaaatgcaaa ctatttgtta
     4741 tatatatgca aaatataata ctaaattaaa ctattaataa aacactatat agccttacaa
     4801 tcatcatttt ttacccctat atgagatgat actatagtta taatacttag tactataaat
     4861 actacaaaac cttttttata tccatcaact atattatatg aatttacttg taaagagata
     4921 gctataatca aattaataat tccacttcct ataaattcaa aagtattaat aacactagtt
     4981 gccagtccag aataaatttc tttattttta tacttggcat cattaaaaac ctgtagatgt
     5041 gacataacta tacatcccat aataaagaat gatattggta aaactataat tggtggtttt
     5101 acctggtata caactacaat tacaatccat ataaacaaat ctatcattgc tccaaatttt
     5161 attatattaa atttgctaga ccttatttta gcaaatacaa aatccataat tattgaacca
     5221 aatataaatc catatgtaaa aaatgatact ataaacgcag aaaaactctt actcacacca
     5281 taaacgtcca ttatgtatct tactccccac aagcttataa atgcacttgt aagaccaaca
     5341 aaagagaaca ttatcattga attataccat gttgatttgt tctttataac agacttaata
     5401 ccatcaacaa tattaacctt ctctgacttt tcatagggtt cagtatctac attgaatcca
     5461 tactcttttg gtgtatctct aacaataata tatataaaac atccaattac aacaccaatt
     5521 ataccgatta ataaaaatga atttctccaa ccaacaagtt cagacaaaaa cacaagtgga
     5581 aaagtagcaa gaactccacc taaatttcct acaaatgaaa atttagctgt tgcagaagca
     5641 aattctgact tattaaacca tctcccttga atttttaaaa tacaaagtaa tattactgat
     5701 gttcctgccc ctacaattac acgagaaata tatgctaatt ctatactttg aatcaaacca
     5761 aataaaattg accctaaact tgccattatt attccaagac tgctaatttt tcttgctcca
     5821 tatttatcaa tcaaaattcc tgcaggtatt tgcataaatg catatgcata taggcaaaag
     5881 gatgctatat ttgaaatttg tatagaatta aaacctaact catttgctaa attgtctgaa
     5941 acaacagcag ttgacatcct taaaaataat actaaaacaa atgaaaatgt aattataccc
     6001 caaacactcc acttagcctt tgtataactt ttcatatctt ctccctcaaa tccatatatt
     6061 tttaaatttt cttgatataa cttatataaa gtgtatagtt attcttacaa atttttacac
     6121 gcaatatcat tatacaacat atgtatatat ttaactagaa ataaaatact attcatatta
     6181 tatacatgaa ttaactacat aaataataag agccatcttt taacattact caaaagatgg
     6241 ctccatataa aaacatttat tctaaattta attttttatc taatataaat tttgtagcga
     6301 aaaataacgc tattgcaact acaagactac caagtattgc aagcataaaa ctaggactct
     6361 gataaagatg atataccata tttgtatctt catttggtaa gatgcttcca actgcatctc
     6421 caacaattga tattactata tttatagcta taaaagaagc aaatgccact atgtttctat
     6481 gtttattgaa ttttggtaat tgtcccattg agattgatgt atataataac aaaataaatg
     6541 atgaatatga aataaacatt actactacta ataataaaat atctaaaaag ttttcagaaa
     6601 tccatttaaa actagtgtta aacaaatcta aaatttcagg tagtaaaatt ccagaagtac
     6661 caaaaagcat aagaactcca aaagtgaata ctgcaactat aaaactaagt acagcgtata
     6721 taagtgctgt tatacattta gataatataa gtgaactagt gctaacagga agagtaaaca
     6781 ttaaataacc ttcatccccc aaaagatttt ttctaaacct ttgaattgta actacaatag
     6841 ttaaaactcc taatgccata aaaagacttg ttaatactag tattcctatt ccctgtacct
     6901 gaagtatatt tgtattcata aatattccat taaatacagc tactatcaat atggctatat
     6961 ataacggtat aaatattctt ccacttgctt ttaactcata ttttaataat ttacctaaca
     7021 tttaaattcc tccctaaata atgcatctat agattttcct ttttcttctc ttattgcttc
     7081 aacgtctcct tgtaacacta tttctccttt tgatatgaat attatctcat cacatatgtt
     7141 ttctatttca cttattaagt gggtagcaat cagtaaagta ctatcttcag aataattttt
     7201 taatatagtc ttaagtatat atgaccttgc agctgggtca acaccaccta taggttcatc
     7261 caatatatat atgctcgcat ttcttgacat tactaaaata agttgaactt tttctttagt
     7321 tcctttagac attgtcttta gcttttcatt tacatcaata tctaagcttt taatcatctc
     7381 atttgctctt ctaacatcaa aatcactata aaaatcatag aaaaacttaa gtaaatctga
     7441 taccttcatc caatcattta aataagttct ttctggtaaa tatgaaacta tttttttagt
     7501 atctatggat ggtttcattc catttatcat aatttcacca ttatcaggct gtaataatcc
     7561 attcattagc ttaatcattg tacttttacc acttccatta ggccctaaca gcccgactat
     7621 ttttcccttt ggtatattta aatcaatatt ttttaaaaca ttcttagttc cataactctt
     7681 gtttacacct ttaaattcaa ctattccatc ttgagaaaca ttgcttatat ttttatccat
     7741 tttataattc tcctttcaga ttttctgtaa taagttttag catttcatct ctatcatatc
     7801 caagcttttc taaggttact tttaatgtat ttatctccac attagctatt tcctctttca
     7861 tagcttttat tttttcctta tcatctgtta caaatcttcc tttagttctc tgactataaa
     7921 ctaaaccttg tctttcaagt tctgttagag ccttttgcat tgtatttgga ttaacttccg
     7981 catcttctgc tagtgctctc actgtttcta atttagatcc tattttaatt tcccctgaaa
     8041 ttatttttaa ctttaaatgt tctactaatt gtatatatat aggtttgtta ttatcaagtt
     8101 cccattccat gttcatgcct ccttattata ttattgtatt aatttattaa tacaataata
     8161 taataataca aagtatatgt caattacttt ttagtaaaag tataaaaaat ttcacaaaaa
     8221 aaatgaacac tacattagag tccattttta acacaattta ttaaattctt tatattacag
     8281 attaattacc ctcttaccaa gttggataca attaaatgtt caaattcttc aattggcatt
     8341 ggctttccaa acaaatatcc ttgtgccata tcacacttta cttcttttaa aagtttaaac
     8401 tgttcttctg tctcaactcc ttctgatatg actttcatac ccaactcttt tgccatttct
     8461 attactttag taacaattat tttgcttctt ttactattat aagtttccat aataaatttt
     8521 tggtcaagtt ttaagatatc cataggaatt tcttttaaca tatttaatga tgagtaccct
     8581 gaaccaaaat catccataga tattaaaaat cctatttttt ttaaattatt cattatatca
     8641 attaaaatat caaaattatc aaatatgata ctctccgtta attcaagttc tatatacttt
     8701 gcaggtatat tgtatttatc aattatattt ttgtatgttt ctataaaatt attgcaatat
     8761 aaatgtattc ttgaaacatt aactgaaaca gtaactggat ttattcctgc atcaatccat
     8821 tttctaaggt atatacatac ttcttcaagc acatataaat ctaaatttat aacaaaaccg
     8881 tttctttcaa aaaatgggat aaattcattt ggaggtatta gccctttatc aggacgtttc
     8941 cacctaacaa gtgcttctga accttgtatc tctcctgtat taagatctat cttaggttgt
     9001 aagtatataa taaactcttt attttctaaa cttgaaaaca ttgtattttc catctctttt
     9061 tcttttatct cttggtcatg aatcttatta tcataaaatg catatgaact tttatgtcct
     9121 ccctttgcca ttttatgagg tatattagcc ctatctatga ttgagttgat atctctatct
     9181 tctggaagta taaaatagat tccacaactt aaaactaact tatacttttc ttcttgatta
     9241 ttattaaaca agtctaattc ctcaaatata gtttttaacc tctttgttat atcttcttct
     9301 tctatatatt tcataattat aataaaatta tccgcagata ttcttgcaaa catttcctct
     9361 tcaaatatat tactcttaat aatttttgtt aaattacgca aaatttcatt cccaacttca
     9421 tatccaaata aatcatttat atatttaaat ttatcaatat ctatatataa taaagcatac
     9481 tttacttgag tatttttaca tagtattttg ttagcatcaa ttacaaattt atctatagta
     9541 tttgctcctg taagattatc tgtatatgct atcttttgca atttaatttt aaaatttaat
     9601 ttgtcatata ttatatagaa tataagcatc atacatatta tagccataat aagtattatt
     9661 tgtattgcat atttgctaat aaactgatta ttagacacaa tagactcaaa gttatttatg
     9721 gagttgtttt tatagagttt gcataaagaa tagactggtg ttgtgtatgt aatttgtaaa
     9781 attatacaaa taaataaaaa aaacatcaat tttttttgta aattcctcat aaaaatttcc
     9841 tcccattaaa acacctctat tttattagca aataagtaca tctcaaaagt ttccatttct
     9901 tgtaaaatgg atagttt
//
LOCUS       DACTGW010000143         9785 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.143, whole genome shotgun sequence.
ACCESSION   DACTGW010000143 DACTGW010000000
VERSION     DACTGW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.143"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            146..1474
                     /locus_tag="I9573_002608"
     CDS             146..1474
                     /locus_tag="I9573_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HAT4797218.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            1502..2827
                     /locus_tag="I9573_002609"
     CDS             1502..2827
                     /locus_tag="I9573_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAT4797219.1"
                     /translation="MSISRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRI
                     FAEVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAA
                     DKEIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLD
                     EIVENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAID
                     TYFEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTE
                     QDTKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAK
                     TNQRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTD
                     IRPPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            2862..3476
                     /gene="plsY"
                     /locus_tag="I9573_002610"
     CDS             2862..3476
                     /gene="plsY"
                     /locus_tag="I9573_002610"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAT4797220.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            3507..4526
                     /locus_tag="I9573_002611"
     CDS             3507..4526
                     /locus_tag="I9573_002611"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAT4797221.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVINKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(4683..5333)
                     /locus_tag="I9573_002612"
     CDS             complement(4683..5333)
                     /locus_tag="I9573_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT4797222.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSGDDELTMKMISSIEYIIKHAK"
     gene            5652..6791
                     /locus_tag="I9573_002613"
     CDS             5652..6791
                     /locus_tag="I9573_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAT4797223.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            6843..8252
                     /locus_tag="I9573_002614"
     CDS             6843..8252
                     /locus_tag="I9573_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT4797224.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            8436..9731
                     /locus_tag="I9573_002615"
     CDS             8436..9731
                     /locus_tag="I9573_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4797225.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNKSNENTTKVS"
BASE COUNT         3725 a         1133 c         1771 g         3156 t
ORIGIN      
        1 tacttatatt gactattaga attaaaatgt gttattatga catgaaatgc ttaaaaatgc
       61 tatcataaaa tataataaat aaataaattg catattaaaa tatattataa taagcattga
      121 tgaatacaaa agagtggtga aaatattgga agtaaatgta aaaaaagtaa ataatattat
      181 aagtaaggtt tataaagata gtattgcaga agaaataggt atagaggtag gagatttact
      241 tatatctgtg aatgaacagc caatacatga tataatagag tatagattct tactaagtga
      301 tgaatattta gatgtagaaa tacaaaaaaa agatggcgaa gtctacatat acgagataga
      361 aaaagactat gatgaagatt tgggtgttga atttacaaat ccgataattg accaagctaa
      421 aagctgtaga aataagtgta tgttttgttt tatagaccaa ttgccagaag gtatgagaga
      481 aactctttat tttaaagatg atgattcgag attatctttt ttacaaggaa attttgtcac
      541 acttacaaat atgagtgaag aagatataaa taatattata aaatatagaa taagtcctat
      601 aaatatatct gtgcatacaa ctaatccaga gttaagacaa aaaatgataa gcaataaatt
      661 tgcaggaaaa ttatatggta taatgaagag attggcagat gctcatatag aaatgaactg
      721 tcaaattgtt ttatgtccag gtataaatga tggaaaggaa ttggatagaa caataaaaga
      781 actagctcaa ctttatccat atgtaaacag tgttgcgata gttccagttg ggattacaaa
      841 gcatagagaa aatctagtag aattaaatat atttaatgat aagagcgcaa gtaaaactat
      901 agagcaaata catcaaatac aacagaaata tcttgaaaaa ttaggtactc gatttgcatt
      961 tttatcagat gaattttata tactatctaa tagtgaattg cctggatatg aagaatatga
     1021 aggtttttta cagtttgaag atggtgtagg tatgatacgt aagttaaaaa cagagattga
     1081 agagtatcta aatatattgc ctgagaatat tctaaaaaga gagaagaaag tatcaatagc
     1141 tactggtcat tctgcatatg aatttataca gagtatggca gatgctatga tggacaagtt
     1201 taaaaatctt caagtaaatg tatatgaaat caaaaacaag ttttttggag agacaataac
     1261 tgtatcaggt cttttaacag ctaaggattt aaaagaacaa cttgaagata aagaattagg
     1321 agaagctctt tatataacaa gaagtatgct aaaagcagat gaagaaattt tcttagataa
     1381 tattgaatta aatcaattag aagaacttat gagaataaaa attatccctt gtttgaatga
     1441 aggaaaagac tttgtggata aaattttaaa atagaaaaca aaagatggaa ggagaaataa
     1501 aatgagtata agtaggccag ttgtagctgt agttgggaga ccaaacgttg gtaagtctac
     1561 aatatttaat aaatttgcag gaaaaaggat atcaatagtt gaaaatacac ctggagttac
     1621 aagagataga atatttgctg aagtagagtg gttagataaa tattttacat tagtggatac
     1681 aggaggaatt gaacctgata gcgaagatat aatattatct caaatgagaa atcaagccat
     1741 gcttgcaatg gacatgtcac atgtaatact ttttatagtt gatggtaaag ctggaataac
     1801 agctgctgat aaagaaattg cacaattgct tagaaagaca aaaaaaccag taatcttagt
     1861 ggttaataag attgatagtc aaagtcaatt tgataatata tatgattttt atgagttggg
     1921 atttggaacg ccttttgcag tttcaggagc caatagtatg ggatttggag atttattaga
     1981 tgagatagtt gaaaattttc cagcaggatt agatacagag tatgaagaag atataataag
     2041 agtagctata acaggtaaac caaatgcagg aaaaagttct atactcaata agatacttgg
     2101 agaagaaaga gtaattgtaa gtccaattgc aggaactact agagacgcaa ttgatactta
     2161 ttttgaaaaa aatggacaga aatttcttct aatagataca gcagggctta gaagaaaaag
     2221 taaaatatat gagacaatag aaaaatacag tgtaataaga gctatgagtg ctgtagatag
     2281 agcagatgta gtattgatag taatagatgc cttagaaggt gtaacagaac aagatacaaa
     2341 ggttgcaggt atagcacatg atgaaggaaa aggatgcata tttgtaatca ataaatggga
     2401 tttaatagag aaagataata agactatgag taattacaca aaagatataa aagaaaaatt
     2461 tccatttatg atgtatgctc caatcgtatt tgtttctgca aaaactaatc aaagaatgaa
     2521 caagatacta gatacagttg aatatgtgtc aaatgaacat tctaagagaa tatcaacttc
     2581 agcactgaat gaagtaattg gagaagctgt aatgttaaat caaccaccat ctgacaaagg
     2641 tagaagacta aaaatttact atgggactca aactgacatt agacctccta aaattacatt
     2701 gtttataaat gataaggatt taactcactt ctcatatcaa agataccttg agaataagat
     2761 aagagaaaat tttggatttg aaggaacttc cataaagttt gagtatagac aaaaaaataa
     2821 aaaataaata tcttaaaata taactaaagg ggggaaattc agtggagata tttagttata
     2881 taattattgc tgtagttgca tatcttttag gaaatatatc tacctcatac atagttgcaa
     2941 agcgaattgc tggggttgat ataaggactc aaggttctgg taatgcaggt tcgacaaatg
     3001 tacttaggac acttggaaaa agagcaggag caatgacatt tttaggtgat gttcttaaag
     3061 gtgtaatggc tgttttaata tcagaatttg cagcaagatt ggttggaata gatactcttt
     3121 tagctggata tctggcagtt atatgtgttg tagcaggtca taattggcca gcagttttag
     3181 gatttagagg aggtaaaggt gttgcaactt cactaggtgc aatgctagct gtaaatccag
     3241 taataacact aatgtgtcta gcagtattta tattggtggt agctataaca aagtatgttt
     3301 cattgggttc tgttgtagga ataggatgtt caccaatatt tatgattatg gtaaaaaata
     3361 aagctggttt aatagtagct ctgttcttaa ctgcatcagt aatatataat cacagagcaa
     3421 atataaagcg attattaaat gggacagaga gaaaaattgg tcaaaagaaa gaatagtaaa
     3481 tatattatac tcaggtggtg ttaaacatgg agaaagtatg tgtattaggt actggaagct
     3541 ggggaagtgc attagcttta gggcttgcaa aaaaaggtaa tgatgtgagc atgtggactc
     3601 gaaaagagga acaagctaaa aaaataaaca gaactaaaga aaatacagac tatttaccag
     3661 gagtattatt tccaaacaat ataactataa gtactgatat agaaaaaact ataaaagatt
     3721 gtaaggtaat agttttagca gttccatcac aggctgtaag aagtacttgc caaaaaataa
     3781 aaccatttat aaaagaagga caagttattg ttaatgtagc taaaggtctt gaaaaaggga
     3841 ctgggcttag attatctcaa gtctgtgaag aagaattacc acaaaatcca tatgtaatac
     3901 tatctgggcc atctcatgct gaagaagtag caagggatat tcctactacc gtagtagttg
     3961 cttctaaaga tttaaaaata gcacagatga tacaagattt atttatgagc cctaaactta
     4021 gagtttatac gaatccagac atagttggag tagaacttgg aggagcatta aaaaatatta
     4081 tagcatttgg agctgggata tgtgacggat tagggtatgg tgataatgca aaagctgccc
     4141 ttatgacaag aggtattagt gaaatgagca gacttggaat tgctatggga gctaatatgt
     4201 ctacctttgc aggtctttca ggtattggag atttaatagt tacttgtaca agtatgcata
     4261 gtagaaatag aagagctggt atactcatag gtaaaggtat gagtttagaa gatactttaa
     4321 aagaagtaaa gatggttgta gaaggcataa ctgctactga agtagcacat gatgttgctg
     4381 aaaaattaga tatagatatg cctataacta atgctatata ttctgtaata aataaaggtt
     4441 caaatcctaa agaagttgga atagaactaa tgatgagaag taaaaagcat gaaatggaag
     4501 aagtagtttt aggtgatgat atataaacta ttgtataata aataataaat ttgttttagg
     4561 ggtgtctcaa aatgaacttt ttagtttacg aggcactttt ttttatatga aattaaatat
     4621 atttttatta tttcaacaat aaaaaaggct tatctctatt ttgagacagc catattttaa
     4681 ttttatttag catgttttat tatgtattca atagaagaaa tcattttcat agttaattca
     4741 tcatcaccag aattttttcc tgtgttcttt tttaagaata atagattttg ataaaaagaa
     4801 ataattatat cattgattat gtctaaatct ttagctggta tatatccagt atctacaaga
     4861 ttgtttaata gtatcttatt tctttccttt atactttctc ctgtaatcat taaagatatg
     4921 tcactatcat gaatacctaa atcttctgga aacatatcat aatattgttt tgttatatag
     4981 ctaagttcat cactagacaa gttaaaaaat acctgataaa aagcttgagc attgtcaaaa
     5041 gaatgcttac aaaataattt ccacataatt atatatttct tatattcatc attgacattt
     5101 tctatacatc ttgaaatatt caagttataa tctttaaaat attttataga tgcaaatgat
     5161 attaaatggt ctaaattttt aaagtattta tatagagttg aactattata tccagccata
     5221 tctgcaacct tacgaagtgt aatattttct ataccttcgt tttccataat attttgagca
     5281 gcttctatga aatacatcaa tatccttttc ttttgtaatt ctttatttgt cattttattc
     5341 acctacaaat aatcaatata gtttatataa tcgtgatttt atcattaaac ttaccataaa
     5401 tcaagaataa tattccaaaa tacaatgtat actcgttata ttttagataa tttaaaatca
     5461 ctagaatata aaaaaatttt aaaaataaaa tcgtgattga caaaataatc atgattatga
     5521 taaaataatc acaaataaaa ttaatttaca tgcaaatacg acaagtacta aaaatattaa
     5581 gaattttaaa ataatatatt tatccttgtt gtattgaata aattatctga atattaagaa
     5641 agaggtgcat tatgtcaaat aatatgagac tagaaagatt gagaaagtac atgaatgaaa
     5701 aaagcattga aattgcttta atatttgaac cagacaatca attttatatt agtggattta
     5761 aagcaataac ttattctaga ccaattgtaa cagtagtaac tcaagataaa atagaattaa
     5821 tagtacctgg attagaagaa ttacatgcta aagaagttgc aaaagttgat aatgtatatg
     5881 tgtattatga aataccagaa atgaaagaac atggaatatc acataaacat tacttagata
     5941 taattttaaa taaataccct aaaggaacaa ctgttggaat agaaaaagat atagtatctg
     6001 caagtttttc agagtatata actgataagt catttgctat caaagatgtt ggaagtaaaa
     6061 tatttgaaat gagatatgta aaagatgcaa aagaaataga atttttgaag atagcaggtt
     6121 acctatctga tataggaata aaagggtctt tagaaaatgt tagagttggg atgagtgaat
     6181 tggagtttga cgttgcgggg gataatgctt tactaaaata tgtttctgaa aattatcctg
     6241 atacatatat aggatttgca aactggactt gttcagggat tgatagaaca gcacaacctc
     6301 atttagattc caatactagg atacttcaaa gaggagatat agtaatacat agtagacagg
     6361 tttggtatga aaactataga gcagaaaatg aaagaacttt tattataggt aaaccaacag
     6421 aaagacaaaa agaagtgttt aagattgcag tagaggctca acaagctgga ttagatacaa
     6481 taaaagctgg tataccagca agaatggtgg atgaagcagc aagagctgtt gttgcaaagt
     6541 atggtctaga gttatattca aatcatagaa ttggtcatgg tttaggttta tcagaacatg
     6601 aagagccata tttaagattt gacaatgaat taatattaga agaaggaatg gtattttcta
     6661 tggagccagg gatatatata cctggagttg gaggatttag acactcagat acagcaatag
     6721 taggtaagaa tggtgctaca attataacta attatccaag aagtgttgaa gaacttatac
     6781 ttgatattta aatcaataca ttgtgtatga gtttgaataa atttgaaatt ggaggaatta
     6841 cagtgaaaaa gaaaattgga ttttgggatt tagtatttat gaatatatca gcactatttg
     6901 ggattagatg gatagctaaa tctactgcct ctagttttgg attgggtctt ggttctatac
     6961 cagtttggtt attgtttgct tttatattct ttgttcctgc atcacttata tgtgcagagt
     7021 tggctgcaac atatccaaaa gatggtggac ttggagaatg ggttaaacaa gcctatggag
     7081 agaagtgggg atttatggtg tcatggctaa attggacatc aaagatattt tggtattctt
     7141 ctttcttgac atttttagct ataaatattg catatatgct tggaaatcca gatttatcaa
     7201 acaataagat gtttatttta gcactttcac tcataatatt ttggctgttg tccttagtaa
     7261 gtactcgtgg gatggcattt ggaaaatttt ttacgaatac aggagcatta ggttctacaa
     7321 ttcctgctat attacttata gtgatggcat ttatgtctgt agttattctc aaaaaagctc
     7381 catcagcatc gatatataca atccaaaata taataccaaa aatagatgcc aattcattag
     7441 tttctatatc tgctataata tttgcattat caggagctga aacgactgct aatttcataa
     7501 ccgaaatgga taatgctaag aaaaactttc ctaaagctat attaactgtg gctgttttaa
     7561 taggaggaat ctatatctta ggttcagtag caataacaat gatacttcct ccagataaaa
     7621 ttgcagcgtc tacagggata ctagatgcat tagctaaagt tgcacaagat ttaggtatag
     7681 gaagttggtt tataagaata gtagcatttg gaataacttt atcagtacta ggagcattaa
     7741 tactttatat agcaggtcca gtaaagatgc tttttggaaa tgtaagagaa ggtgtatttc
     7801 ctaagcaatt gactgttaca aataaataca atataccatc aaatgcagta atagttcagg
     7861 ctataatagt gagtctactt cttgtaggaa caaacttatt accatcagta gataatatat
     7921 acaatgttct tgttactatg acagcattaa cagcattatt tccatatgtc ttgcttttta
     7981 catcttatat tagacttaga aagacaagac caaatgaaga aagaccatat tcaattgcaa
     8041 aaagtgattc agtttgtatc aatattgcca gaatggtact tgtagtaact gtagttggca
     8101 tacttttctc aacggcacca gttatggaga ctttaaaaga taatataatc tatgagattg
     8161 aaatgatagg aggaggtctt tttgttataa taacaggttt aatgctatgg aagaactatg
     8221 aaaaaaagat atctaataac tctggacgtt agtataaata aaaatataat tattatggtg
     8281 agctaaaaga gtttactttt agctcataac tcaccataaa ttattgatag attaacctaa
     8341 tatattaata aatattccga aatattgaat aaacaataaa ataagcaata attaattatc
     8401 atgaaaattt acaggtattt acataaggag gaagtatgtc agttgaagta aataaaccaa
     8461 gcatttggaa aaatttcaaa gtcttgataa tactctttgt agcaggagca tttatatatg
     8521 ctttaccata tttaaagaat tattattatg atacttttgc acaagcattt aatcttacaa
     8581 atacacaaat gggaagctta ggaagcattt atggtctact tgcaatggta tcataccttt
     8641 ttggtggttt tttagcagac cgtatttcag ccagaaaatt gctttcaggt tctttaatta
     8701 ttacaggaat atctggtctt gtattaacac tttatcctcc atatattgtt gtgtttttaa
     8761 tccatgcctt atggggaata acaacaatac ttactttttg gccagctctt gtaaaagctg
     8821 ttagaatgtt agcatctgaa aatgaacaag gaaaagcttt tggatttatg gaaagtggtc
     8881 gtggaattac caatgcaatt catctatctc tagtattgat actatttggg tatatttcat
     8941 caaaagtaag tgataaatct ggtcttacag ctattatagt attatactcg gcaataaatg
     9001 taatagttgg agtattagta tttttcaaat taaaggataa tgaaaaagag gtatcagcaa
     9061 aatttgataa agaagccttt tttgcagtaa taaaaatgcc tcatacatgg ataataacaa
     9121 taataatgtt ttgttcatat tcagctaata tgtcatttta ttattttacg ccttattcaa
     9181 ctgaagcatt tggagctact gtggtgtttg catctgcaat ctcaatatta gctcagtatt
     9241 gtagaccagt agcatcagct gcttctggat tacttggaga taaagtaggt tcatcaaaga
     9301 ttatatctta tggatttata cttatgattg caggtctttt aggtgtaata tttacaccaa
     9361 caaaatcatc tatgatttat atactattga ttggatgtgt tgcaatatat gtatctatgt
     9421 atgcaattca aggtttacac tattcgttac ttgaagaagg taattattca ttatcaatat
     9481 caggaactgc tataggaata gttgctacat taggatatct tccagaagta ctttgtcctt
     9541 tagcagcagg gaagatattg gatgcatttc cgggtgtaac tggatatagg tactacttca
     9601 tagttcttac tatagtagcc ataattggat tggtgtttac atttatatgg atgaacatga
     9661 caaaagaaag acgtaaagaa cttattgaga tgaataataa gtcaaatgaa aatactacta
     9721 aagtcagtta atataataca ctactatttg gaccagcaat attaatttaa tttacattga
     9781 aattt
//
LOCUS       DACTGW010000144         9652 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.144, whole genome shotgun sequence.
ACCESSION   DACTGW010000144 DACTGW010000000
VERSION     DACTGW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.144"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            288..1652
                     /locus_tag="I9573_002616"
     CDS             288..1652
                     /locus_tag="I9573_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAT4797226.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            1674..2165
                     /locus_tag="I9573_002617"
     CDS             1674..2165
                     /locus_tag="I9573_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAT4797227.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            2438..3109
                     /locus_tag="I9573_002618"
     CDS             2438..3109
                     /locus_tag="I9573_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAT4797228.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            3194..4060
                     /locus_tag="I9573_002619"
     CDS             3194..4060
                     /locus_tag="I9573_002619"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAT4797229.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            4093..5595
                     /locus_tag="I9573_002620"
     CDS             4093..5595
                     /locus_tag="I9573_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HAT4797230.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSVVLAGLTGIPVNVLIVV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFIGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            5725..6177
                     /locus_tag="I9573_002621"
     CDS             5725..6177
                     /locus_tag="I9573_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAT4797231.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            6337..7140
                     /locus_tag="I9573_002622"
     CDS             6337..7140
                     /locus_tag="I9573_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAT4797232.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            7198..8085
                     /locus_tag="I9573_002623"
     CDS             7198..8085
                     /locus_tag="I9573_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAT4797233.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEVWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNEKDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            8104..8952
                     /locus_tag="I9573_002624"
     CDS             8104..8952
                     /locus_tag="I9573_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT4797234.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            9015..>9652
                     /locus_tag="I9573_002625"
     CDS             9015..>9652
                     /locus_tag="I9573_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAT4797235.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKAKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITFDSSKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDN"
BASE COUNT         3895 a          918 c         1687 g         3152 t
ORIGIN      
        1 aatttattag ctaatgttga gtaactttat taattaatac tttactataa ttaagattta
       61 cagatagttt gctgctgaaa ataaagaata gaataaattt aaattaagca taataaaata
      121 taaataataa agttcgcatt aacaaactat ttaaatatca agacctgtta aaaaacaaca
      181 gcaataaaca aataaaatag ataaatatat ccaattaagg tataattaag ttttatatga
      241 tatagttata tttgataatt tacaatagta aaacttggag ggaaaaaatg ggtgaaaaga
      301 aaataaataa tatgaattat tcacataata attattccaa aaagacaggt gaaccaacta
      361 gaaaaataaa aaatagccat atcactctta aaagtaaaga agaaaaaatt tgttctagtt
      421 gcaataaaga aaataatact aaggataatt actgtaaatt ttgtggaaat gaactttatg
      481 aaatagcatc attaagacca cttgaaacaa aactagactt gaaatctaaa ataaaagagc
      541 tttcatatca tgcaaataaa agaggtgtat ttttaacaac atttacaacc atatttatat
      601 tgtttattat tgcattgata tttaaagcta ttataactat tcaatttaat gatattagtt
      661 atttaataaa tccagcacat ataatcctag ctctcaactt gggtcaaatt agtgtatcta
      721 tgtctaccat gatgggttct gggtttataa atgccaatat aggtttgttg atattactta
      781 taatccctat tttggtatta ttaatatcaa acctaatttt tatgagaaaa agatgtagag
      841 attcaaaaac tgtattagca aattctctag gagttggaat tttttatgga ttagttttag
      901 ctattttatc tatttttact aatgtaaaaa ctagttcaca tagtatgtta gaatatggat
      961 atgcattaga atatagttat gaatttttta gtgttctttt aaatggattt gtattagggt
     1021 ttatatgtac atacataact acttataaaa aaagttatga aaaggaaaat atgtatttat
     1081 ctcttttttc aaattctata agaacattca tattaggtta tgttttagta ttagtaatac
     1141 tattggtact aactatatct gatagtagtt acttaaatga actagatatg agtagttatt
     1201 caaatggatt aaatttattt actatactcc cacaaatagc tagttatatg tgggcttttg
     1261 caaatggaat ttcagtaacc ataataaact caacagtatc aatgtttact ttaagttctt
     1321 catctttatt tggagatact aaattaatgt tttatgcaat gggagcattg tctatgttaa
     1381 tattattatt aaatggatac aagctaagat ttaagtataa tacagatagt ataagaccta
     1441 taatagtatt tagtatatat tatgcatttt tgatgggaat attggcttta tttagtacat
     1501 ttatactaga tagtaatatt aatttcttta atacaactaa ttatggaaca actttaataa
     1561 tgcaatttaa agtcttacaa gctattgtaa tttcatttgt atatagtttt gtgatatctt
     1621 taataggata taagctaaat tcagctgatt aaatatgatt ggagggaatt tctgtggaag
     1681 gaaatactaa taataaagta gatgataaat tttctaaggt taagaaaaag tttggtagtg
     1741 gaaaatttaa agttgaatta tataaagaaa aatcaaaatt aaatagagag attgatgata
     1801 ttcaagaaaa gaaagcaaag atattcttgg aaatgggaat actgacatat caaaaaatta
     1861 gagaagattc tattaatgat aatagttttg ataaattctg tgatgagtta ctagaacttg
     1921 ataagttaat ttatgaaaaa aatatggaaa ttaatgaaat ggaaatggca gaaagtaatg
     1981 taacttgtga gtgtggttat gttggaaatg taaatgataa atattgtgct gaatgtggta
     2041 aaaaatttga attggaaaat gaatgtgatg attttataat ttgtggttat tgtgaaagtg
     2101 agatagatac agaggctgaa ttttgtccat gctgtggaag aaaaattata atggatttag
     2161 aataatattg ggaaacctat gtatagtata catgggtttt tttattgaaa ttatttaaat
     2221 ggacttataa cagtagttat ataaaatgaa aataaacacc aaaaaaatga aataaaattt
     2281 cttttaaatg taaaaaacaa gctaatatat aaaataaatc agtaaaaata tagatgcgag
     2341 aaaaaaatat gaaaaaatat tgcaaagaaa aagttttcat gttattatta atacataaat
     2401 caagaaaata aatttcagaa acgaggtaat ttaaaaaatg ctagataagg taaagggaag
     2461 attaatagta tcatgccaag ctttagaaaa tgaacctctt catagtcctt ttataatggg
     2521 gagaatggct aaggctgcta tggaaggtgg agctgttgga attagagctc aaggagttga
     2581 agatattatt gaaataaaaa aagttactgg acttcctgtt ataggtatta taaagagaaa
     2641 ctatgaagat tcagatatat atataacgcc aactaaaaaa gaagttgatg aacttttaac
     2701 aacagggtgt gaaatgatag cattagatgc taccaataga gttagaccaa ataatgaaga
     2761 cttaaaagaa cttataaagt atataaaaga aaacggtgtt ctagtaatgg cagacatttc
     2821 aaattatgat gaggctatca aagcacaaga gtacggtgta gattgcgttt caactacact
     2881 atctggatat actccatata ctaaaacatt agaaggtcca gattttgtat taatggaaag
     2941 attagttaaa gatttagaaa tacctgtaat agctgaagga aaggtaaata ctcctcaaga
     3001 tttaaaaaag gtctttgagt tgggtgttca ttcatcagta gtaggttcag caataacaag
     3061 accacaatta ataacagaaa aatttgttaa agcaattgaa attaattaaa aattaattta
     3121 taaattaaaa aattattata tttaactata cttgaaaatt aaaattggag gtaaagatat
     3181 gagaacaaca gatatgaagg gaatttattc agcattatta gtttcatttg ataaggaagg
     3241 gaatataaat gaaaaagggt taagacaaat aattagacac aatatagatg tttgtaaagt
     3301 agatggtctt tatgtaggag gaagtacagg ggaaaacttc atgctttcaa ctgatgaaaa
     3361 gaaaagaata tttgaaatag ctaaagatga agtgaaagaa gaaataaaat taatagctca
     3421 agttggttca gttaatttaa aagaagctgt agaacttgct aaatttacaa ctgatttagg
     3481 atatgatgca ataagtgctg ttacaccttt ttactataaa tttgattttg aagaaataaa
     3541 acactattat aatacaataa taaattctgt agataataga ttaataatat actctatacc
     3601 atttttaact ggtgttgata tgagtttaga ccagtttgga gaattatttg aaaatgaaaa
     3661 aataataggt gttaagttta cagcagcaga tttctactta ttagagagaa tgagaaaaac
     3721 attcccaaat aaattaatat ttgcaggatt tgatgaaatg atgttaccag ctacagtttt
     3781 aggtgtagat ggggcaattg gttctacatt caatgtaaac ggtgtcagag ctagacaaat
     3841 attcgaatta actaagaatg aaaaaatatc agaagcactt gaagtacaac atgtgactaa
     3901 tgatttaata acagatatat taggaaatgg cttatatcaa acaattaaat tactacttga
     3961 agaacaaggt gttgaggctg gatactgtag acaacctatg aaagaagcaa ctgatgaaat
     4021 gaaatcaaga gcaaaagaaa tatacagaaa gtatttttaa taaaatactt ttagtatggg
     4081 aggattagtc aaatggtagg atttacttgg atagattttg caatactagt ggtttactta
     4141 ttggcagttt tatttgcagg attgttattt tcaaaaaagg aaatgaaggg aaaagagttc
     4201 ttcaaaggtg atggaactat tccgtggtgg gttacatcag tatcaatatt tgccacatta
     4261 ttaagtccaa tatcattttt atcattagca ggaaattctt atggtggtac ttggattttg
     4321 tggtttgcac aattaggtat gtttatagca atacctttga caataaaatt tttcttacct
     4381 ctgtatagta ggttagaaat agatacagca tatgaatact tagaaatgag atacgaaagt
     4441 aaaggtctta gagtacttgg agctttgatg tttataatat atcaaatagg acgtatgtct
     4501 atcataatgt atcttccatc agtggttcta gctggtctta caggaatacc tgtaaatgta
     4561 ttgatagtag ttatgggtgt aatagcaata atttattcat atacaggtgg attaaaggct
     4621 gtcttgtgga cagattttat acaaggtatg gtattaatag taggtgttac agggacattg
     4681 ttctatttaa tagcaagtat aaatggtggt tttggaacag taatggatac tcttacaact
     4741 ggacacaagt tcttagcaag taatgaagtt atgtttgata aaaatatact atctacaagt
     4801 gcatttataa tatttattgg agctggtctt aatacttttt catcatatgt atcgagtcaa
     4861 gatatagttc aaagatttac tacaacaact gatataaagc aacttaataa aatgacatat
     4921 ggaaatggtg ttttatcaat gggacttgca acagtatttt atctaattgg aacttgttta
     4981 tttatatact atactcaaaa cccagattta gctcaaacag ttcaacaaga ccaagtattt
     5041 gcatcatata tagcttatga attaccagtg ggtataacag gaatactact tgcagcgata
     5101 tatgcagctt ctcaatcaac tttatcgaca gggcttaatt cagttgctac aagttggact
     5161 ttagatatac aaactatcat aacaaaagat ataagtatgg aaaagcaaac taagatagca
     5221 caatatatat cattaggtgt aggtatactt tctatagctg ttgcaatagt attagcaaat
     5281 ggagaaataa agtcagcata tgaatggttt aatagtttta tggggctagt actaggtgtt
     5341 ttagcaggaa tatttgtact tggtgcattt tgtaaaaaag cgactaaaat gggagcatat
     5401 gttggattca tagtatcagc aattttagtt gtttatttaa aataccgtat gccagaagta
     5461 acttcttggg catattctct aataacaatc acgacttctg tagtggtagg tacaatagtt
     5521 agtgcaatag aagctaaagt taaagcaaaa gtatctttac caaaagctga gactactgtt
     5581 tattatgaaa aataatattt aaatgataaa ttgtcttata gagtgattag ataaataatt
     5641 tagttactct ataagattgt ataatatact ttgtatataa aagtataaaa atagacttta
     5701 taattatata gggagatagt gcttatgata tttggaaata taaatcatag taagacatat
     5761 tcaacattgc atgaagattt acttagatgt tttgaatatg ctaaagataa ttcattaata
     5821 gattatgaaa aaggtactta tacaatagat ggtgatgata tttttgtgaa tattgtagag
     5881 tataaaactt gtgaaaaaga agatagattc tgggaagctc ataagaagta tatagatgtt
     5941 catttgatgc ttgatggatg tgaacgaata gatataaatt ttatagaaaa tttagaacaa
     6001 aaaatatatg aagaaaaggg cgatttcttg cctcttgatg gtaaaaataa tggatacatg
     6061 gaacttagaa aaggtgattt tttagtttgt tatccagaag atgctcatat gacaggaata
     6121 aaagttttag aaaaagaaaa tatcaaaaaa gctattttta aggttttggt tagataatga
     6181 taatttatat aaccactagg gctgaaaata gtgatttaac tttcagcgat tagtgagact
     6241 tcgtaaattt tagttagatt actattttca gccttagttt tttatgagaa aattgaaaat
     6301 aattaaaagc ttaaaatttt gtaaaggagt taacttatgg aaaatataga tgtattctta
     6361 ttaataggac aaagcaatgc tagaggattg ggaaatccaa aagagagcat cattccaaat
     6421 gaaaattgtt ttgaatattt gagtactgat gaaattataa atatgagatg tgagctagag
     6481 acttcagaag gtgatggtac tatagcacct gcattttcaa atgaatggaa taagcttact
     6541 ggaaataaag tttgttttat tcacaatgca aaagatggtt caagaataaa aaactggaat
     6601 catgataata actggtttct aaatgacact atagaaaaat ttaatacagg ctgtactacc
     6661 ttaagtaaac attataatat agaaaataaa tacgttattt ggattcaagg tgaaagtgat
     6721 gcaaaatatg gtagtgatgc attgtactat aaagaaagtc taaaaaaaat tgcgtatagg
     6781 cttaaagaag aatgtatgat tgataaaatg tttgtaagtt taactggtta ttggcttgga
     6841 gaagatgaat attttattag aacaagacga attgcagcag ctcaagaatc tgcttgtaat
     6901 gaatgtgata tactgtgtgt aggaagtaaa attgctatgc aatttcatga cagaggatta
     6961 actattgatg atgttcatta cactcaagaa ggtcttaata tgttaggaga ggacttatgt
     7021 aaaaatattt ataagtatca tataaccaaa gaaaaaagtg tattaagtga tactatagac
     7081 ttatcagaag caaggaaata tatctgtgaa ttggaaaaga taagtaaaag atttgtgtaa
     7141 tataaaattt taaatagagt tgttaattta taaaaataat ttgtatggag atatgttatg
     7201 aaaaattacg tttgtataga tgttggaggt acttcaataa aatatggttt tattagagaa
     7261 gatggattta taattgataa atcaagtctt gatactgaag caaaggaaaa aggtggagaa
     7321 ggaatattag ccaaaataaa agatatagta aaaaaatata tagaggaaaa tgaaataagt
     7381 ggtatatgta tatcaactgc aggtatggtt gacccagttg aaggtaaaat actatttgca
     7441 ttagaagaat taattccaaa ttataagggt atgcaattaa aaaaagaagt agaaaaagag
     7501 tttaatataa aatgtgaagt tgaaaatgat gttaattgtg ctggacttgg tgaagtgtgg
     7561 cttggcgcag gtagaggtgc aacatcttct atatgtttaa caataggtac tggaattggt
     7621 ggatgtatta taataaacaa taaactgata aatgggttta gtaatagcgc tggtgaagta
     7681 ggatatatga atataaatgg aagttctttt caagaattgg catctactag cagtctaatt
     7741 aaaaaagtag caaaaataaa aaatttgaat gaaaaggact taaatggaaa aataattttt
     7801 gatttagcaa agaataatga tgaagattgt ttaaaagaac tcgataatat gataaaatca
     7861 ttagcagttg gaatcgcaaa tatatgttat gtaataaatc cagaagtggt aattttaggt
     7921 ggtgggatta tggcacaaga aaagttccta aaaccaaaaa tagacaaagc attaaaggag
     7981 gttcttatag agagagttta caaaaacact aatatagaat ttgcaaagag acaaaatgat
     8041 gcaggaatgc ttggagcatt atataatttt ttaaacaaaa tataaagaat agaagggttt
     8101 ttaatgagta ttttagaaca attggaatca ccaactttta aagtcacaaa atcagataaa
     8161 ttattaatag cttatataaa agaaaatata gatgatgtat tttataaacc aatatcacaa
     8221 atagctaaag agagcaatat tggtgaagca actataacta ggtttgtaaa aaaaatgaat
     8281 tttaatggat tacaggattt taaagttact ttagcacaag aaatatcaac tatcaataaa
     8341 aaaaatatta taaataaaaa tatacaaaat gatgagccag ctttggatac agcaaaaaaa
     8401 cttttaagtt caaatgtaac cactttagag aacacagtag agataatcaa tagtaaggat
     8461 gtacatgact gtgcaaggct tataataaat gcaaagaaag tttacttcat aggcattgga
     8521 tattctggta taatagcaca ggattcaaac tataaattta tgagaatagg ccttaattgt
     8581 gtaagttttg atagcagtca tacgatgata atgatgtctt ccataatgga agaaggagat
     8641 ttaataattg caatttcaca ttctggtgaa acagaagaga ttataaaaac tgtaaagctt
     8701 gcaagagcca ataatgctaa aattatatca attactgaaa ataaaaattc tgaattaaag
     8761 gatatttcag atgtacactt atcttatgta tcaggagaaa cagtactgga aactggttca
     8821 atatcttcta aattagcaca atttttcatt attgatttag tatatacaca ggtagtaaaa
     8881 gaactttcta atgaagctat tgaaaggaaa attaaaacta caaatgctat aaaattgttt
     8941 aaaaatgaat aaaataagaa atttgtaatg ttttatagag ctctcttaaa aatttaggaa
     9001 tggagagata ttacatgtct aaattaaaag attataatat tgataaagta ataattttta
     9061 ataatttaag tactaaaacg aagaatcaac ttaaagaaaa agctaaacta aaaagattac
     9121 aaaaaaaaga aattttattt tatgaaaaag atagcttaga cagtgtttat atacttttag
     9181 aaggaaaggt atctattttt aagataagtg aaaatggaga gagaaaagta attttcatat
     9241 taaatagtgg ggaagtcata aatgatatta catttgactc ttctaaaaac tccactgttg
     9301 gctgtgaagc atttgaaaat tcaattgtag cttattatag tattgaagaa tttttagaaa
     9361 ttatgcaaaa tgattttcaa cttacaaagg atattatttc ttatatggaa agaagaataa
     9421 gacgtttata tagacagttg aaaaattcta tatccataaa agtagataaa aaacttgcag
     9481 caaaattata taggttaagt agagaatttg gagtttgctg tggagaatgg actttactaa
     9541 atgtaaatat tacagttaca tatttagctg atatgcttgg ctgtaaaagg gagactgttt
     9601 ccagaatgat aaaggtattg caaaaagaag aacttattaa gattgataat aa
//
LOCUS       DACTGW010000145         9646 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.145, whole genome shotgun sequence.
ACCESSION   DACTGW010000145 DACTGW010000000
VERSION     DACTGW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.145"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            932..2290
                     /locus_tag="I9573_002626"
     CDS             932..2290
                     /locus_tag="I9573_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine permease"
                     /protein_id="HAT4797236.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     ASGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2396..3190
                     /locus_tag="I9573_002627"
     CDS             2396..3190
                     /locus_tag="I9573_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAT4797237.1"
                     /translation="MVTIKEYSIPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3183..5489
                     /locus_tag="I9573_002628"
     CDS             3183..5489
                     /locus_tag="I9573_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAT4797238.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVAESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRIIRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5628..6905
                     /gene="tnpB"
                     /locus_tag="I9573_002629"
     CDS             5628..6905
                     /gene="tnpB"
                     /locus_tag="I9573_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAT4797239.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIESLKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7088..7810
                     /gene="yqeC"
                     /locus_tag="I9573_002630"
     CDS             7088..7810
                     /gene="yqeC"
                     /locus_tag="I9573_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HAT4797240.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            7917..8486
                     /gene="mocA"
                     /locus_tag="I9573_002631"
     CDS             7917..8486
                     /gene="mocA"
                     /locus_tag="I9573_002631"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HAT4797241.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            8483..9502
                     /locus_tag="I9573_002632"
     CDS             8483..9502
                     /locus_tag="I9573_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HAT4797242.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT         3810 a         1007 c         1810 g         3019 t
ORIGIN      
        1 atacaaatat agtaaaggag agtacattgg tacaactgag atacatagct tattaagttt
       61 ttctgtctta tgagaattat attataaaat attcataaat attttgagtt taaattaaat
      121 aattatttta tgcttagatt aattgatatt taattaattt aataacaaaa acagtgaaag
      181 agtaaataat attatttaca gggggtaaag caaagtatgt taaataaatt gtacttttgg
      241 ccccgtacat agagaaatct atgtaggaca caactttaaa accagtaaat cctaaagcct
      301 tgcaactaca ataaaagata gaaagaaact tttatgaatg tggtgaaaac cagaaacaat
      361 agtgaggatg gtatatggtg agagcctaag tacttaaaaa tggacatttg gtcgccaagc
      421 ctagaaatag gaaggtcaaa cgactatctc gagagagagt acactataag cgattgatag
      481 tggaaacggg ttgctcttag caggtaatgc tgaagatgaa gaaatagtct acgctcatat
      541 gaaagtatga ggtcactaga tttgaagtga catctataaa gtagcgttta taggtaaatg
      601 tttaaaactt gaaacatgta aatacttatg aaaacttata acttataatg attaagattg
      661 aaagatttaa attgaattaa atttactata aaaaattaaa aattcatagc tataatactc
      721 ttatatttgt aaagcatggt atataatgta tacaaaatta aagaatacgt gtatttgtta
      781 ataattaaat ttattgcatc tgttttttaa gactaaaaac ttaaatatat gttggagtat
      841 agttgatatt tatgaacatc attaatttaa tatattagat ttttaagtat ttatataatt
      901 caataagttt tatgtaacgg gagtttttat tatgaaagca aaaagtacat caaaatttga
      961 attagacgga ataccaccac taaaggaagc aataccttta gggttacaac acttaatggc
     1021 aatggtaata ggtagttgtt taccagcgat attgattgca aatgcatctg gacttaatca
     1081 taagatgtca acattattga ttcaagccgc attagttttt gcaggattat caactttatt
     1141 acaattatat ccaatacctt tgtttaaagg ttttaaatta ggaggaggaa taccagttat
     1201 aatgggagct acagctatgt ttataggagc tggggtatct gttgcaagta agtatggact
     1261 accagtatta tttggctctc aaataatagc tggtatagta ataattataa ttggttttgg
     1321 tcttaaaaaa atacgattta tatttactcc agctgtatca ggaacaatag ttgcatgtat
     1381 gggtcttgga ctttttccaa tagctataaa aaacttagct gggggtatgg gcaatgagac
     1441 ttttgggaat ccaattaatt ttatagttgg aatatctgta tctcttatga tattaggatt
     1501 aaataagtat ggaaaaggat tatttaaaga tgcatcaata ttagttagca taatttttgg
     1561 atacatttta tcattaattt tgggtatagt aaatttttct tcaattcagg agtttacttt
     1621 ggttgcgctt ccaaaaccac ttgcttttgg gctagatata agacttgaag ttgttgtaat
     1681 gttttcaata atatatttag ttgagatagc tgatataatg ggtgcttgta cactttctgc
     1741 ggtaggaggg cttaatagac aagtaacaga tgaagaactt tcttctgctg ttttaggaaa
     1801 tggagtactc tctacaatag gtgcattatt ttctgcaact ccaatgggga tgtttggaca
     1861 aaattctgca atcgtttcca atacaaaggt tgtaagtaaa tttgtactag ctattggagg
     1921 aattggatta tttttggcag gaatatcgcc tttattagct aatttaataa gaacaattcc
     1981 tccctgtgtg gttggaggag caacactcgt tatattttca actcttacaa catctggttt
     2041 aagacttgtt agtatggatg gatttaatca agaaaatagc atgatattag gtctttctat
     2101 ggcttctggt attggattca tggtagctcc tcaagttctt gaaaaatttc caaaatttat
     2161 agagacatta cttgcagatt caagtgtagt ttcaggagca atggtagcca taataattca
     2221 agctttatat atggtaaaat ttcctggaaa taaaagtgat aaggttgagg ataaaaataa
     2281 aagtttataa tgtaaaggtt taatatatgt gaattaaata tactacaatt ataaatataa
     2341 ataaaagatt tattaaaaac ttttgtgttt taataagtta atgatggggg ataagatggt
     2401 tactataaag gaatattcaa ttccacagag tttagaagaa gcatataaaa ttttaatttc
     2461 taaaaaaaat aatgttattt taggtggatg tggatttata aaattaagta ataaaaatat
     2521 aggaacagcg atagatttaa aagatataaa tctaaattat ataaaagagg atgaaaaaaa
     2581 tattttgata ggtgcagata cttcacttag aagtttagaa attgataaaa ctataaaaaa
     2641 ttactgtagt ggaatacttt caagcgctgt ttctaatata gttggtgttc aatttagaca
     2701 aggggctaga attggtgcaa gtgtattttc aaagtatggc ttttctgact taataccagc
     2761 gcttttggtt gttggagcga aggtgagatt atacaataaa ggaatactag agttaagtga
     2821 gtttttggat agtgaattaa gtagggatat attggtcgaa gtaatactac caaaaaaaga
     2881 tgcgatagct gtttttgact caataagaaa atgcacagga gatttttctg tattaaattc
     2941 agcaatgcta aaagaaaatg atacttacaa aatagcaatt ggggcaagac ctcaaaaagc
     3001 tagattagca cttgaagcga gtaatatatt aaataaagaa aaagatatag ataaggcatc
     3061 tattgttgct agtaaggaac ttacttatgg aagtaatatg agagctagta aagagtacag
     3121 aaaggatatg gcaagtgcat tagttaaaag gatgtataat gctattgagg agggaatgta
     3181 taatgattaa taaggggata agaaagatag atagtacagc aattgtcaca ggaaaacctc
     3241 tatatactga agacctcatt atgcataaag atgttttgac attaaaacta cttagaagtc
     3301 cacatgctca tgctaagata aagaatatag atacatcaaa agccgaaaaa attccagggg
     3361 ttgtgggtat ttatacgtat aaagatgtac cacaggtcag atattgtgtg ccaggtcaaa
     3421 cagaaccaga agcatcacct tatgatagag ttttacttga agatacagtt agatttattg
     3481 gagatgaagt agctttagtg gctgctgaag atgagaaaac agcacttaaa gccttaaaaa
     3541 tgataaaggt agattatgag gttttaaagc ctgtgtttga tgcaagagaa gcagaaggaa
     3601 atgaagttgt tatacatcca gaaaaagaca tagtagagcc ataccctttt ggattggatg
     3661 caaaaaataa tatagtatca aaagaatttt ttgaatttgg agatgttgaa gctaagctta
     3721 aagaaagtga ttttgtggct gaatcttcat acaaaaccca aagtcaatcg cattgcatga
     3781 tggaaactca taggtcatat tcatataaag atataaatga tagaattgtt gtagtttcat
     3841 cattacaatc accacacaat gtaaagagaa taattagaaa agtattgggt ataccatctt
     3901 caaaaataag agtaattaaa cctagaatag gtggtggatt tggaggaaag aatatagcag
     3961 caacagatat atttactgca tttgttacta tgaaaactgg tagaccagca aaatttgtat
     4021 ttacaagaag tgaaactttt gtaggttcta atagtagaca tgaaatgttt tttgatgtta
     4081 aagttggtgc agataaagaa ggaaatttaa aagcaataga gctaaagtca ttaaacaata
     4141 caggtgctta tggtgggaat ggtgtctctg tttcttccga atctggacac aataccttac
     4201 caatatataa tgatattgat gctgttagat ttaatgctag aacagtatat actaataggt
     4261 taccagcagg agcacttaga gggtatggtg ctacacaggg aatatttgca atagatagtg
     4321 ctattgatga attggcacat aagattggaa tggacccact tgaatttaga attaaaaata
     4381 taatagatag gtataccaat ggaaaagcag ctgaagacga aatattaagt tgtgagctga
     4441 gacaatgtat agaaaagggc aagaaattaa tcaattggga tgataaatat ccatgtaaag
     4501 atataggaaa caacaaagta agagcagtag gtatggcagt agcaaatcat ggttctggta
     4561 taccgcgtgt agatatggca acagtcacta taagaatgga tgaagatggc acatataaac
     4621 tattatctgg tcttgctgac ttaggtcaag gggctgatac aattcaagct caaattgctg
     4681 cggaagcttt aaatactact gtagatagaa tttctattta tacaggtgat tcagatttat
     4741 gtccctatga tacaggagca tttgcttctt gtacaacaac ggttacagga aatgcaacaa
     4801 taaagacagc aaataaatta aaagatatat tactttcagt tgcagagtca aagctaggat
     4861 ttaaaaaaga agatttagag ttgggaaaag acagggtttt tgttaaaaac gataatagca
     4921 agtttgttct attagaggaa ttgggtagag aatctgttgg aggaatagga tttgctagtg
     4981 atgatgtacc agtagttttg gaagcaacag aatcttttgg catgttgaaa aaaataaaac
     5041 catttatagc aggatttgca gagatagaat tagataaaaa tacaggcgaa tataaggtca
     5101 taaattttgc atgtgtacct gattgtggaa cagttataaa tccaacttta gctagaatac
     5161 aagtagaagg tggtgtgatg atgggtatag ggtttgcaat gtatgaagac cctatatttg
     5221 ataaagatgg gagattatta acagatagtt tcctacagta taaagttcct acaaaaaaag
     5281 aagttggaaa tatattggta gattttgctg agaatttcga cccaggaggt ccttatggtg
     5341 ctaaatcatt aggagaaata gttatacata caccagcgcc agctatagca aatgcaatat
     5401 ttaatgctac aggagttcgt ttaagagagc ttcctatgac ttttgaaaaa gtttatatgg
     5461 gtatgaaaaa taataataaa caattataat aaataataag gtgtataaaa gcaagtaaat
     5521 ttaaattatt ttgtaaaata aagtaattaa atggacatta tatatggtgt tttgtaaaaa
     5581 taccgtatat aatgtctagt agagtatatt ctaaaggagg tgagttcatg tcaaacagaa
     5641 taaaaaatat acactatgga actcagagac aacaattaaa aaacttaact aaaaaagaat
     5701 atcaagctct acgagagatg tgcttcttag ctaagaactt atataatgtt ggtacatata
     5761 atgtaagaca gcattatttc aatcaaggag aacatcttaa ctatgaagct aattatcatg
     5821 tatgcaaaga aaatgagaac tataagcttc tcaatagtaa tatagcgcaa caaatattaa
     5881 aagaagtaga cagttcgttt aggtcatttt ttgggttaat taaattagcc aaagaaggga
     5941 aatatgactt tagagggata aaatcaccta aatatttaga taaagaagga ttttttagca
     6001 taataatagg tcaaataaga atcaaagaag atgggatttt aaatgtacct atgtctccag
     6061 catttaaaaa actatatggg aaagtaagta taaaagttcc acaaaatata ctagagaaaa
     6121 aaattaaaga aataagaata atacctaaac ataaagctag gttctttgaa attcagtatt
     6181 gttatgaaat accgaaatca gaggaagttt caaatcaaaa aaatgcacta gctatagact
     6241 tgggtttaga aaatttatgt acatgtgtta ccaatttagg tgatagtttt atcatagatg
     6301 gaaaaaggct aaaatctgtg aatcagtggg caaacaaaca gaatgataaa atagaatcct
     6361 taaaattaaa aaagaatata actgcaataa caagaaaaca atttaaagtt tggaataaga
     6421 gaaataatca agttaaagac tatgtaaata aaacctgttt ttatataatt gattattgtg
     6481 taaaaaataa tattggaaat ttaattgtag gatataatgc taagttagaa aaaagtgata
     6541 atatggagag aaaaatatat agaaatttta taagaatccc atttggagag ataaaaggaa
     6601 aattagaata tctatgtaaa ataaaaaata tcaattttat taggcaagaa gaaagctata
     6661 ctagtaagtc tgattttttt gctgatgatt ttctacctga tatcaatgtg gacaatgtta
     6721 aaaaatatga ctttaaaggg gaaagaataa cacgtggaca gtacaaatcc agtgttggaa
     6781 tcattataaa tgcagatgta aatgcgagtc ttaatatact aaaaaaatca caagtagtta
     6841 atttatctaa tttacagaat aatcctcaaa tattaaagca accacagaga gtaagaatat
     6901 cttaattata tttaaaagat aaactgatag cgtaagctta cagcttagcg gcgaattgga
     6961 tccgccttgt tgtttacaac agaatctatt tactttcgag tttatggaat ttcaaaaatc
     7021 acttttacta aaaaggtcta actataataa ttaagagatt agaaaactgg ttataagagg
     7081 tgaaagtgtg gaactatcaa atataataga aaagaatgaa ataataactg tagttggagc
     7141 aggaggaaaa acttcgttta taaactattt tgcaaatttt tatagagata aattgaaagt
     7201 tcttcttaca acaacaacta aaatatatgt accaaacgat tatgacaata taattattac
     7261 aatagatgga actgttattc catctatttg tcatggaata acagtctgtg gcagttatat
     7321 aaataatgaa aataaattag taagtattga ttcctctatt ttggatgaaa ttgtagacca
     7381 atttgattta gttttaatag agggagatgg ctcaaagaga aaaaaactta aaggttggaa
     7441 tgctaaagaa ccagtagtat atcataaaac tacaaaaacc atagggatat tagatataac
     7501 atcatttgga atgaatatta atgaagagaa tatacataga gtagaaatat ttaaaaaaat
     7561 agcaaattta gatacaagta gtattaatag tagttctaca gtaagtattg aaaacttaaa
     7621 aaatatagta ttaaatccaa atggattatt taaaaattat tctggaaaaa gagtattatt
     7681 tattaataaa gttgaaaatg aaaaatataa aaatttagct ataaaattaa tagaaaatat
     7741 taaagaatat gaaagtgaaa tagagatatt ttatggaagc gtaaaacaaa aattttgtgt
     7801 aagatattga agtattaaaa tagtttatat gatattatat atatagataa tatcaataga
     7861 gatattataa aaatcataga actatcaata tattataaat accaaggatg atatatatga
     7921 taaatgcagt gataatggca tcaggctttg ccagaagaat gggagcaaat aaacttttat
     7981 taaattataa aggctgttct ataatagaac atgtatttag agaagttagc aagattgatt
     8041 ttcatcaagt tgtagtagta agtcaatatg aagaggtttt gttattgtgt aataaatatg
     8101 gttttgaata tatagataat aaaaatgcaa atataggtca aagtgaatct ataaaattag
     8161 gagttttaaa tagctcaaaa tgtgatggtt atatgttctt tgtaggagac cagccattta
     8221 ttgataactt atatatagat aaaattataa atacttttaa attagataga gattttatag
     8281 taattccaag atacaaagat atgtgtggaa atccagtcat attcccatat agtaagaaag
     8341 aagaattgtt ggcacttaaa gaagatgaaa aaggtaaaac cattttaaaa aattcatcaa
     8401 aaataaagta tgtagaagtt cctaaagaaa tgttatttga tatcgataca aaaatggact
     8461 atgagagaat cgggggtaaa ttatgaagct aataaaaact atagatgctg ttggacaagt
     8521 tttatgtcac gacataactc aaataatacc tggagaattt aaaggaagaa aatttaaaaa
     8581 gggtcacata gtaaaagaag aagatattcc agtgttatta tctcttggta aagataattt
     8641 gtatgtatgg gaaaagtcag aaggaatggt tcatgaaaat gaaggcgcat tgtttttaaa
     8701 agaactaact gctggagaaa atttagattt tagtgaaata aaagaaggaa aaatagattt
     8761 tatagcagca tgtgatggtc tattaaagat agatgttgat gcactttttg atttgaattg
     8821 tgttggagaa ataatgatgg ctactcttca taataatttt atagtaaata aaggtttaaa
     8881 agtagctgga acaagagtga tacctttggt tatagatgag aaaaaattag aagatgcaaa
     8941 aaaagtagtt ggtaatagaa aaatagtaaa tgtagtacct tttaaaccta agaaagtagg
     9001 aattgttact acaggaaatg aagtgtttta tagcagaatt gttgataaat ttggaccagt
     9061 aatagaagaa aaagtaaaag gctttggttg tgaagttata ggacagacta tatgcccaga
     9121 tgataaagaa attataaaga gtgcaataaa agaatttata agtcaaggag cagaacttat
     9181 ctgttgtact ggcgggatga gtgtagaccc agatgatgtt acgccaacag ctataaaaga
     9241 aactggagct gatttagtga catatggttc accaatatta ccaggagcta tgtttttact
     9301 tgcttattat ggagaagttc caattatggg tataccagga tgcgcaatgt atcataaaac
     9361 tacagtattt gatatagttt tatctagggt tttaattgat gaaaagcttg ataagtatga
     9421 tatagctaga tatggtcatg gtggcctttg tatgaactgt gatgtatgta cttatcctgc
     9481 atgtaatttt gctaaaatat aaagttatta aaaaagagta tttacaaatt aagtactctt
     9541 tttaaatatg aaacaaaaca gctagaatga tgttttaaga aaagcatatt tatttagaaa
     9601 ttataataac ttattttcaa tgtgtaaaag aatgaataaa gttgca
//
LOCUS       DACTGW010000146         9643 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.146, whole genome shotgun sequence.
ACCESSION   DACTGW010000146 DACTGW010000000
VERSION     DACTGW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.146"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(132..1418)
                     /locus_tag="I9573_002633"
     CDS             complement(132..1418)
                     /locus_tag="I9573_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAT4797243.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(1433..2698)
                     /locus_tag="I9573_002634"
     CDS             complement(1433..2698)
                     /locus_tag="I9573_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAT4797244.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            3014..4111
                     /gene="ychF"
                     /locus_tag="I9573_002635"
     CDS             3014..4111
                     /gene="ychF"
                     /locus_tag="I9573_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAT4797245.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            complement(4590..6869)
                     /locus_tag="I9573_002636"
     CDS             complement(4590..6869)
                     /locus_tag="I9573_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT4797246.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITEKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYIARSNGNIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFAQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(7140..8594)
                     /locus_tag="I9573_002637"
     CDS             complement(7140..8594)
                     /locus_tag="I9573_002637"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAT4797247.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYNSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            8758..9627
                     /locus_tag="I9573_002638"
     CDS             8758..9627
                     /locus_tag="I9573_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT4797248.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGYLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
BASE COUNT         3326 a         1504 c         1033 g         3780 t
ORIGIN      
        1 tattccttat ctatagctct taaattttta cctcaattta tataaatata ttttataaat
       61 ttgttttacc aaaataaaca tttaaaaaca taaataatac ttaattagtt attcttatta
      121 tgattaagtt tctatttagg ttctacaata gaaataacac aatactcttc tttaaaatgc
      181 tcttttaatc tttcttcaac ttcctcaaaa gtaatatctt taataacatc taaataatcc
      241 aatagattta tatccttaaa aacataagat ataaaactat tacctataaa gtttatagaa
      301 tcaaagcact taataaatga ccctattttt ttctttttag ttctctcaaa ctcttctttt
      361 gataatcctt ctttttttga tttttctata taatcaagaa ttatttcttt tactttttta
      421 ggctctttag aatctccagc tattatgcta aaagcataat ccacttgaga cgaaaatcca
      481 gctccaaaat tttcatttat taaaccttgc atatacaaat cttcatagag cttacttcct
      541 tttttaaata acatccctac aagtatatca gttacaatct ctttacgtaa aagttctttc
      601 ccttttaaac caacattact atctttaaaa cctatgttga acataggcat agatataggg
      661 aatttttcta taacctcttt ttcttttaca ctttctggtt cttctggata aaatctttct
      721 atactcttag agagtttgtc tactttgtaa ttgtttgact ttttagttac atctataact
      781 ttttctacat ctaagtcacc aacaacaaat aaagccatat ttcctggatt atagaaagta
      841 ttataacatt tatatagctc ttccttagtt atcttatata tactatcaac agtacctgct
      901 atatcaattc tagcaggata atttacatac attgccttca agcaattaaa gtatacattc
      961 caatcagggt catcattgta catttttatc tcttgtgcta ttataccttt ttccttttca
     1021 acattttcat ctgtaaaata aggtgtttga acatagtcaa ttagatgttc taaactctca
     1081 taaaaatttt cagtagcaga aaataagtag gctgtcattg taaaattagt aaatgcattt
     1141 gcatttactc ctaattttga aaatttatca aatgcatctc ctccatcagg ttgctcaaac
     1201 atcttatgct ctagaaaatg tgcaatacct tcattcactc taattttctt atcttctcca
     1261 attggtacaa attctaagtc atttgaacca taattagtag ccaaaatagc atactttttc
     1321 ataaaacccc ttttaggcat aaaatataca tctaatccat tttgcaattt ctcataataa
     1381 acttcttctt tcaatatatc attaactatt ttttccatag acatctcctc cattaatttc
     1441 tcaagaagta tacagtatca agctctatat tttttatagc ttcaactata tctttttttg
     1501 taaccttttc tatactagat attatgtctt gtacttcaga atttgtcttt gccatagact
     1561 gagagaaaac aaaatcagac ataccaccta tattatcagt tatagatttc atagaattaa
     1621 ctaaagcaag tttactgttt tctaattctt catcagatat atcacctgct tttacacttt
     1681 ctacttgttt ttttatcaat tctacagctt tatcataatc tttagtttct ataccagatg
     1741 atataaacat gatactttta tacttctcta ttgaagagaa tatatagtaa cataaacttt
     1801 ctttttctct tacattaaca aataatttag agtgtggacc accacctaag acattgctcc
     1861 caactactaa agcataatat ttatctatgt ctgcataatc cacatttgct ctataaccca
     1921 taactaactt accttgagtt atttccattt gttcatctat aacttttacc tcatccacat
     1981 tttttataaa atctgctctt ggtatattta taatttcttc tctttcaaat tttagatttt
     2041 tacttattat atctacaact ttatcttcat caaaattacc ttctacaact atatctatag
     2101 gtgatgtttt aagtatgttt ttataatgtt catataactc tcttgaagtt atactatcta
     2161 tttcatcttc ataaccaaat tcacttacac tatatctctc tcctttacac atagcctcaa
     2221 tacatttatc ttgcgcatat tcctttttat catttattat tgattgtatc ttttctctta
     2281 aattctcttt ttcaatattt aaatattctt ctttaaaccc accatctaca acaagcgtat
     2341 tatttattac ttcattaaaa aattctacta cttcttcaaa tatactctca tctaaatact
     2401 tttcacttat gttaattact ttaaaactca atacttgacg ttctccacgt tttacagcat
     2461 cagctcccat agatgaacca tataaatcat ctaatttatt ggatattgct ctagcactag
     2521 gatatttagc gcttccactt gctattattg atggtattag agcatttttt gtagtttctt
     2581 tttcatccaa tagtctctga acatataaac ttataagatt cgatttaaac ttttctgttt
     2641 ttatcaaagt aagattaata ttattaccta aatttattat ttttttagta ttaaccatat
     2701 gtacctccta tttttattta aacatcaatt ttaaaattga aatttattta ttttttaatt
     2761 atatatactc acatataaaa accttctatt taattatatc ctttttatta ttttctaaaa
     2821 gcaatatttt tatttaaatt aaatgcaaat attatttaaa tcaatatgct ttaaagtcta
     2881 gtttacaagc ttgatatata tgatgtataa tcaatatgta tgtctataat taaaacaatt
     2941 ttgcaatcct atagattgat tgtttaaagt tatataaaga atataaaaaa gaataagaga
     3001 gaggacgatt aatatgaaat taggtatagt tggtttgcca aatgtaggta aaagcacact
     3061 atttaacgca ataactcaag caggtgcaga atctgcaaat tacccttttt gtacaataga
     3121 cccaaatgtg ggagttgtat ctgttccaga tgaaagatta aacaaactac aagaattata
     3181 taattctgaa aaaatagttc ctactgctat agaattttgt gatatagcag gtttagttag
     3241 aggtgcttct aaaggagaag gtttaggaaa taaattctta tcacatataa gagaagttga
     3301 tgcaatagtt catgttgtta gatgttttga ggatgaaaat gttgtacatg tagatggctc
     3361 tgtagaccca cttcgcgata tagaaactat aaacttagaa ttaattttct cagatataga
     3421 gattcttgaa agaagaatta ataaaactca aaaagctgct aaggctgata aaactttagg
     3481 ttctgaactt gatttattaa aatcaataat gtctacttta gaagaaagta agtgtgtaag
     3541 aactatggaa tttacagaag atgaacaaac attcgtaaat tcattggatt tacttacatc
     3601 taagcctgta atatatgctt caaatgtatc agaagatgat ttagctgata atggagaaaa
     3661 taataaatat gttcaacaag ttaaagcatt tgcagaaaca gaagatgccg aagttgttgt
     3721 agtatgtgca caaatagaag cagaaatttc tgaacttgat tctgctgaag aaaagaaaga
     3781 gtttttagaa acattaggtc ttgaacaatc agggcttgat aagcttataa aatcttctta
     3841 tgctttactt ggtttaattt cattcttaac tgctggtcca aaagaagtta gagcatggac
     3901 tataaaagtt ggaagtaaag ctcctcaggc aggaggaaaa atacattctg atatagaaag
     3961 aggatttatc agagcagaaa caatagcctt taatgatttg gttgagcatg gtactatggc
     4021 tgctgctaaa gaaaaagggt tggttaaatt agaaggtaaa gaatacatag ttaaagatgg
     4081 agatgtaata ttatttagat ttaatgttta aaatgattaa tcatatacac tttgactatt
     4141 atatataata actaaaaaac taagattata ataatacaat attatcttat aataattata
     4201 tagaatttaa aaataaaaga agagtatttc aaatgatttg tataaattat tatgaaaaac
     4261 tctttttttg atataataaa attacttaca tatataacat ttaaatttat aaattatctt
     4321 tttatttttt aaaatatgta taacacacct atagaaatta ctattattac tatctaaagt
     4381 attttgctat aatcccatat actcttacct caaaaacatt caaatggaaa aaatactaca
     4441 tattaataaa ctttgcccaa taattcctaa aataatatcc taacaatgat ttatgttttt
     4501 ataatagtat atttttacat aaaaaagtga acattgatta acaaagttca cttttttata
     4561 aatttttatg ttataattca caattttatt taatttgttt cttttctatt taaactatat
     4621 ctatctaata ttaatttttc atattcttct attggcattg gtctagcaaa taaaaatcct
     4681 tgggccatat cacaacctat aaaccttaaa aactctgctt gctctacagt ttcaacacct
     4741 tctgaaacaa cttttatatc aagttgcttt gccatttgaa caatacttga aactacaatt
     4801 tgactctttt ttgcatttcc cactgttata aagaattgcc tatctaattt caatacatca
     4861 attggcatat ctttaagcat atttaaagaa gaataaccag aaccaaaatc atccatagaa
     4921 attttaaatc caacagattt caaagtttgc attattttaa ataatatttt tgtattgtca
     4981 aatattgcac tttctgtcaa ttcaatctca attaaatttg gttcaatttc atattctttt
     5041 gttattcttt caagctcaga aataaaacct ggattctcaa gatgtactct tgataagttt
     5101 attgatatag gaaatgtggg gaaattttca ttttcccaac gtttaaaatt tttacaaact
     5161 tgggcaaata caaacatatc caattctgtt ataaaaccat ttttctcaaa aataggaata
     5221 aatacacctg gacttattag accttttttg ggatgttgcc aacgtacaag cgcttcagca
     5281 cctacaattt tagtagttga taaatcaacc ttaggctgta aataaacttt aaattgttca
     5341 ttttttaaag catcatgcat ttcttgttca atatcacgtt cttctatgag agtttctttt
     5401 aaattttcag cataaatctc ataaactttt aaatattttc tttttactgt agtttttgca
     5461 atcatagctt tgtcaagaca ggtagaaata tttgtttctc cctcttcaac aaaatataca
     5521 cctatggatg gaattaaatt gagttctact cccaaacttg tttttatcat tgacaatttt
     5581 ttacgtataa gttcacatat ttctataatt gactctctat tatttttctt ttcaaatatt
     5641 atagcaaaat tgtcatttga aattcttgaa aatacagatt gctctttaaa tatattctta
     5701 attatctctg ctatttcctt taatactcta tctccttctt catacccaaa cctatcattt
     5761 actagcttaa acttatctat atcaaaataa attaaaacta gattcttttt ctcatgagag
     5821 gataaaaatt ttcctccttc aagattaaat ttatttgaat tgccaatacc tgtaacgtta
     5881 tctttaaaag caaattcttc caatttttcc ttactctttt tatgtataaa tagtatataa
     5941 attgttaatg ccgtaaagat tataatcaac actacacatg ctgataatgt aagttttata
     6001 atagcgtatg atttctcaga tatagcagtt ttaggaactt cagaaaaaag ataccaatca
     6061 ttgattccta ttggggcata acttaaataa tatccttttc caaacatact ataacttaat
     6121 gaacctgatt taaagttttt tatatttgac ttcatattat tgatactaga ttttgatatt
     6181 gaatcacttc tgccaatgta agaaaataaa ttttctttag tagagttggc gtttttgctg
     6241 cttggattta aaacaatatt accattactt ctagctatat aagaatatcc ttttccatta
     6301 aatgtaggta cagaaagtat tttttcatac aacttcgtac tatgtgtggc aaatataact
     6361 cctttaacct cattatcttt ataaattggc acagaatata cattaatatt actattggta
     6421 tttttatcat cttctattac atcttttaat ttttctgtta taaccacttc tccttgcatt
     6481 gattttaaga aataatctct tttagagaag tcttctatat aaccatctgt agtgtatgct
     6541 gtaccatttg gtaaaataac tcccattctt ttaaatgaac tattaatagc ttgtcttttt
     6601 agtatagaaa ggatattatc tacttcaaaa gactcttctc cctcaataaa tattgaaatt
     6661 gactctagta actttatatc tccattaatt tgttttttta acacattgac gctttgattt
     6721 gctatttctg aaagatattg attcgtttct tcacctaatg tcttattcaa ccttgaacca
     6781 taaatcaaac aggaaattgt aattactaag atacttatga cagcaattat ggacaaacca
     6841 tacctattat tttttgcatc acttaacatt atgcttcctg cctttctcga atctatgtat
     6901 gtataatcat tatcttatga caattattgt aaacatttta taataatact tatttttcca
     6961 tatttactgt aattatacaa aattataaac tttattacaa caataaatta attaatatag
     7021 atattagggt ttatattatt gcattatcta ttttacaatc atttacatat aaaaaaatgt
     7081 aatatcaaag tgaaacttga tattacattt ttgttcttgt attttgttta atttttatgc
     7141 tacattttac ttaaacactc atttgcaact tctttatcat caaaatgatg cttaacttta
     7201 cctataattt gataagtttc atgtccctta cctgttataa ttacaacatc atcctttttt
     7261 gccatactta tagcttctgc tatagcttgt tctctatcaa caacaacatg gtaattttct
     7321 tttttcttgt caattccttc aagtatatct tttattattg cctctggttc ttcagttctt
     7381 ggattatcag aagttataat acataaatca gaatattttt gtccaattgc tcccatcaaa
     7441 ggtctctttt cagtatctct atctccacca catccaaaca cagaaattat ttttccttct
     7501 gcaaattgtt gagttgtctt tagtacattt tcaagtgcat caggagtatg agcatagtct
     7561 acaattacac ttataccttt atcatttgga actgtttcaa accttcctga tactccatta
     7621 gaaagtctaa gaccttcttt atatattggt ttaggtatgc caagtacata gcaagcagct
     7681 ataactgcta aagtattata tactgtaaac attcctggaa ctggtataaa tattacttct
     7741 tcatatgaag gtgttattaa tctataagat actccatccg aatctgattt tatatctcta
     7801 gccataaaat cagccttagt atcaacacca taagtgtaac aaggtacatt tatacctttt
     7861 atattttcat atatcttttt tccaccttca tcatctatgt ttattatatt tgccatagta
     7921 gtcttaaaaa ataatttttc ttttgctttt ctataatcct ctaaatcttt atgaaaatct
     7981 aaatggtctg gtgttaaatt tgtaaatatg cctagtttaa agtctgtttc atcaactcta
     8041 tttaatgcca atgaatgtga tgaaacttcc atcgcacagt agtcacatcc attatccaac
     8101 atattattaa aatgatattg taaatctata ctttctggag ttgttgaatt ggaaacaatg
     8161 tctttttctc cgtcaaaaat ttttatagtt cctataagtc caactttatt cttatttaaa
     8221 gttaatatct catttagtat agtagttata ctagtttttc cattagtacc tgtaactcct
     8281 ataacgttaa atttctgaga tggatgatta taaaaattat cagctacttt tgccatatct
     8341 tttcttgtat cttttacctt tataaatgta catcctttta tttctacatc ttcttcaaca
     8401 agaaatgcac ttgctccctt ttctatggca tcttttatat acttatgacc atctgacaca
     8461 aatcctttta tacatataaa taatgtacct tttgtaacct ttcttgaatt atattgtaca
     8521 ttattaatat ctatatttaa ttcacccttg acattgatta tatccaaccc ctgtataata
     8581 tcatttaatt tcataaatgc tttcctccct ttaaactctt tctgctaaaa ttacactaca
     8641 tataatatta tattaagaat tttttactat gtgaatggtt aaaaatgctt gaaatattgc
     8701 aaaaaatgct ctataatttt ctataatata actgaggaaa ggtcttctat aaaaactatg
     8761 gataataaaa tcgggtactt aaacgataac tttaaaatat ttaatattag agataaaaaa
     8821 gatatacaat ttgaatacca taatcatgat tttaataaga taattatatt tataaatgga
     8881 aatgtaactt attttgttga tggtattccc tataaactaa agccttggga tatactcttt
     8941 atcagcaata atgaaatcca taaaccagtt atagattcaa atgtctttta tgagagaatt
     9001 gcaatatgga ttagcccaga atttatgaaa aaaaatagtg atgctactag tgacttagaa
     9061 aaatgtttta aaatagctac ggagaataaa tgcaatagat tgcgtttaaa tatacatgat
     9121 attgaatcta ttaagagttt tattgagcaa ataaatattt ccgaatccaa tactgaattt
     9181 ggaaatgaaa tattgagaaa tgcattattt atacaactta tgatattctt aaacagaatt
     9241 ttcctaaaaa atgaaaatat ggaatcaaca gaagacgttt tttatgatga aactattaag
     9301 aaagtattaa actatataaa ttcaaatata gatgataagc tttcaataga tagcatatcg
     9361 tctgaatttt ttataagtaa gtattatctt atgagaaaat ttaaatctca tactggaacg
     9421 tctatacaca attatatagt tcaaaaaaga ttaattttag caaagtcact aataaccaat
     9481 ggttacctta tgagtgaagt ttgtaataaa tgtggattca acgattattc tagttttgta
     9541 agggcattta aaaaaaacta cggtgtatct cctaaaaatt atataaacaa taaaaatcct
     9601 ttagatatta ctcttttaga tgaatagata atttatgtaa att
//
LOCUS       DACTGW010000147         9587 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.147, whole genome shotgun sequence.
ACCESSION   DACTGW010000147 DACTGW010000000
VERSION     DACTGW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.147"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            235..909
                     /gene="fsa"
                     /locus_tag="I9573_002639"
     CDS             235..909
                     /gene="fsa"
                     /locus_tag="I9573_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAT4797249.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILDEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1031..2425)
                     /locus_tag="I9573_002640"
     CDS             complement(1031..2425)
                     /locus_tag="I9573_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAT4797250.1"
                     /translation="MGIILKPKQFYMTVIVTIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(2503..3849)
                     /locus_tag="I9573_002641"
     CDS             complement(2503..3849)
                     /locus_tag="I9573_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT4797251.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGIQQMLFSIGNMTLQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGYWKTKAIVNHRINE"
     regulatory      4099..4201
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            4295..5671
                     /locus_tag="I9573_002642"
     CDS             4295..5671
                     /locus_tag="I9573_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAT4797252.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCLGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFM
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(5785..6930)
                     /locus_tag="I9573_002643"
     CDS             complement(5785..6930)
                     /locus_tag="I9573_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAT4797253.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     SRGNRSGNTISIITKKELDIIKDLQKEFSIVITPKTLQNGELIDIIK"
     gene            7182..7889
                     /locus_tag="I9573_002644"
     CDS             7182..7889
                     /locus_tag="I9573_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797254.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            7936..9531
                     /locus_tag="I9573_002645"
     CDS             7936..9531
                     /locus_tag="I9573_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797255.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADSREKQKIMGMILSVVIITG
                     LLIFYVTSISLSLVDILKQINQMEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFFNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFMFIGQISSRIKYKNLILIIGSIILMLLYTLLMTRLNSMSVEMLKN
                     SVSISETIRKIYPPIYYFVDALKTGSVVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKTYKFKSQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIIGTILILL
                     FGADKVGELLKLNIDFNFIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLGFEIQFIIVSICLIASFSMFVALMGLLS
                     NLLYPNLDWKNEMAVVKRSASMIITMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TFILDFMIWRIISTKGVRIFKNL"
BASE COUNT         3512 a         1192 c         1404 g         3479 t
ORIGIN      
        1 tataagataa aatacaatat atcaactatt ttaacatgaa atacaaaatt aatttaatat
       61 aaaaagtatg ttttttctaa ctatatattt agaatttagt aaataaaaaa attataaaac
      121 taagaaggaa aagcaaaaca ataataaaaa ataaaaaaca ataagcaaaa tgttgaaaat
      181 aataccaaat caatatataa taagaataaa gaaagattaa agggggatat tttaatgcaa
      241 ctaatcttag atacaggtaa tgtggaggag attaaggagt tatgtacttg tttaccaata
      301 gatggtgtaa ctacaaatcc aagtattgtt tcaaaagaaa aaaagaattt taagcaactt
      361 ataaatgaaa ttggtgagat tataggtgag gatatgccta tacatgctca agtgttaagt
      421 acaaagtatg aagaaatatt agacgaagca ttatatataa gtagtttgag aaaaaatata
      481 tatgtaaaaa tacctgtaac acaagatggt ttaagagcta taaaagacct tcacagaaaa
      541 ggtgtaaaaa taactgctac tgcaatattc actgctcatc aaggtttttt agctgcaaag
      601 gctggagcaa attatattgc accttatgta aataggcttg ataatatatc tggtgatgga
      661 gtagctatgg tttcagaact tataaaaata atagatactt ataaaatgga tacaaaagta
      721 cttgcagcca gttttaaaaa tgcacaacaa gttatagaat taatgcaaca tggggtgcat
      781 agtgttacag taccatatga tatttgtaaa tctatgatga atcatccatt aactgattgg
      841 agtgtagata agtttataga ggattgggag aatacttttg gaaaaggaag taaaacaaat
      901 aatatttagt attatggtaa aagagtagtt ttgtccaatt tcacaagaac ttatataaaa
      961 agatgaaaat atgaattttc tttttaataa tttcgttatt atcttaagtt aattcatatt
     1021 ttcatctttt ttagatttct agtcctataa atttccacca agggaatgtt atacatatta
     1081 ttataaagta ggttaaagga atcaaagtta aaccgtattt gaaaataacc ttgctattat
     1141 acagattttc accatgacca accattattg tagcatgatg aaatggtaat atgtactgta
     1201 tgcttactat aatgtagaca aatagacata aagtaacact attttccttt agtatatgta
     1261 aacttccaag taatggtatt actacagatg atgtagtgac agcacttcca agacatatat
     1321 tcaagattat tgtgagagtt atcaaaaaca atattgaaat aatttctgaa gagtttggta
     1381 taaactttat caaataattt cccattatat ctacaactcc agagtacttt aacactcctc
     1441 caattgaaaa tgctgctata aaaaaaataa ggaccttcca atttatttct tttaaagtac
     1501 taaatccaac tatttttctt agtaataaaa gaagtacaca aattgacatt ataattaatg
     1561 tgtttatgtt atgtatgaac tgagttaaaa acataaaaaa cattattcct aggcataaaa
     1621 atacgtgttt ttgctctttg tttagcaaat taaattcttt ttttaaatta gaatatgaaa
     1681 aatgaagttt attaccctca agattttcat tagaaataaa ttttattgaa tgcaattctt
     1741 ttttaaagac aaagataaat aaaaaataag ttataaaaca agtaattata gttggaacag
     1801 acatataaaa agcccattga ccccaattta tattacaatt tccaagttct agtacaacat
     1861 aatttagaag ggtgtctcca tttacaaaaa acattgatgt aaaggtagat gctgtaaata
     1921 tactaaataa aagtatagat tttgaatctt cagttatctg ttgttctttt aaaaactcct
     1981 tgtaaaaagc agatacaaga ataacccttg gaaatggttg tgggataaaa aatattaaaa
     2041 gaatacctaa aatatacgat aaaagaatca tctttttagg ggtatttaca gtatttaaaa
     2101 ttaacttctc agaaataatt tttgcgacac ctgtttttgt aactgcatta gtaattatgt
     2161 atgcaagaat tattatgtaa aaattttctg taaataaaaa ctgtaaaata acctttattg
     2221 gtgcaatgtt aaatataaac gctagaatta caaaaaacaa tgcaacaaaa gttttttcaa
     2281 ctgagtctac agcccatatc aagatagtta aaaaagataa gcatatagaa aattttatgt
     2341 tgaaactgta attaagagag aagtagagaa ttatggtaac tataacagtc atataaaatt
     2401 gttttggctt aagaataata cccattttta tattcacctc cataatttat tatatcataa
     2461 tggaggtgaa tacaatgaat gtgaatacat tttataaaaa gattattcat tgattctatg
     2521 atttacaata gctttagttt tccaataccc tttcttatag tagataactg taacaataag
     2581 acctaaagtc caaccaaaag gaatacccat ccaaattccg tctgttccaa aaaatttaga
     2641 aagcatataa gaagctggaa gtcttccaag ccataaagat actattgagc ttataagtgc
     2701 aaacatggaa tcaccagcac ctttcattgc acttgaaagt acaaaagaag ttccaataaa
     2761 taaaaagaat ggtcctatta taaataagta agaagagcca attttaatga catctttatc
     2821 agtattgaat agagcaatta gattttccct aaatagcaaa attaaagcaa taactgtaac
     2881 tgctaaaact aaggtcattt ttatagattc tcgaatgcct tttttaaccc tttcattttc
     2941 tccagcacct atgttttgag caacaaaagt agaaacagca gaaccaagat tcataatagg
     3001 taagcttata aaattttcta tcctaagacc agcaccaaaa gctgccatag cagatgtacc
     3061 ataactatta actaaaaatt gaagagtcat atttccaatt gaaaacaaca tttgttgaat
     3121 tccacttgga agtcctaatc taagtccatc tttaaatatt ttgttatcgt atacaattcc
     3181 tttgatttta aatttaagca cttcatgttt tttatttaaa taaattatac ttattataaa
     3241 tgatactgcc tgagccatta tagtggcaag tgcaacgccc tcaacaccca ttcttagcac
     3301 tacaataaaa gttaagtcta aaactatatt tagaattgtg gctattatca gaaaataaag
     3361 tggagttact gaatctccaa gtcctctaag tattgcactt actgaattat atccagcaga
     3421 aaataatatt ccactaaata ttatagtaag atacaatttt gattgagcaa aaacggattc
     3481 tggtgtattc ataagcttaa gcattgggcc accaaatact attccaagaa tacttattaa
     3541 tatagaacca ataaataaaa gtatatatgt agtatcaata gtagctttta ccttttttaa
     3601 atctcctgag ccataaaatt gtgacactaa aatagaaaat ccaaggctaa cacccataag
     3661 cgctgccacc aataaaaaca ttatagggtt agctccagag actgccgcca ttgcttcttt
     3721 tccgataaat ctaccaacta ttatactgtc agctgtattg tatagttgtt gaaataaact
     3781 accaataagc ataggcatgg caaaaaagaa tatggatttt ccctcatgac ctgtagttaa
     3841 atctttcacc ttattcctcc aaataaaaaa attattaatt tattatagta taatcttaac
     3901 ataaacttta tatccaaact ataaataaat tgtatcaaaa tatcatatat ttttttaaaa
     3961 tgtaaagaat ataattataa tttgaattta ataaatcaat atttaatata atcgaatatt
     4021 aatacaaaat caatacaata tatacgtata tattgacaaa attaattata taaattaaaa
     4081 taatattcgt aaagaaaaat tcatgttttg tactgtataa catataattt tgacaatatg
     4141 ggtcataagt ttctaccgga ataccgtaaa tattctgact atgtatacta ttatttagtt
     4201 aattaaatat ttaattaact gaattatgaa ggatacggct cagcttatca actactgtgt
     4261 cgtttttttg taaaatttaa taggaggtgt tgttatgcaa acaacaaaca gtagtactat
     4321 gcagaaactt tttccaattt tgactaataa aaatgtcgac atgaagaagg aaataattgc
     4381 aggagtaact acttttttaa ctatggctta tataatagct gtaaatccaa atattttatc
     4441 tgagacaggc atgcctgcag gtgcacttgt tacaggtact tgtttagcag cagcatttgg
     4501 atgtatttta atgggtgtag ttgctaacct tccttttgct ttagcatcag gtatgggtct
     4561 taatgcattt tttgcatata cagtagtttt acaaatgggt gttccttggg aagtagctct
     4621 tacagcagta tttgtagaag gtattatttt tatagtatta tcagtaagtg gagttcgtga
     4681 ggctgttgta aatgcgatac caaaaaatat gaaattagct gttacaggtg gtataggaat
     4741 atttattgca cttataggtc tagttaacag tggaattgtt attggagacc aagccactct
     4801 tatcaaaatg ggtagattta caccagcagt aataataact tgtttaggat taataattat
     4861 tgctgtatta gataaaaaaa gagttaaagg ttctatactt tttggaatag tagtgagttc
     4921 attacttgct tgggcatttg cttttatgaa tccagagtat gcacaaaaat taggtatata
     4981 tttacctggt ggattattta aattcgaaag tttagcacca atagcaggta aaatagattt
     5041 aggatatgta ctacatccaa caaatatagg tggtttttta gtagtagttt gtacattttt
     5101 atttgttgat ttttttgata cagtaggtac attagttggt gtttgttcta aagcaaacat
     5161 gttagatgaa aaagggaatg ttcctaatgt aggtagagcc ttacttacag atgcggttgc
     5221 aactacagtt ggtgcaggac ttggagtatc tacagttaca acttatgttg agagttcaac
     5281 aggagttatc gcaggtggaa gaactggatg gacagcaata acagttggaa ttttattctt
     5341 agctgcaatg tttttctctc ctgtatttat agcaattcca tcatgtgcta cagcaccagc
     5401 tttaatatat gttggatatt taatgcttgg tacagttaag gatatagagt ttgataatat
     5461 aactgaaggt gttccagctt ttgttactat tgcttgtatg gcattaacat atagtatagg
     5521 tgatggttta actttaggta tattaactta tgttttcgta aatatatttt ataatatatt
     5581 tggagctaaa aaggcagaag acaaaaaaca tgtttctgta gtaatgattg ttctggcaat
     5641 attatttata ataaaacttt ggctattata ataggtatta tttaaaattt taatttaata
     5701 taaaataaag ggctataatt tattatcaat tgtgatgata aactacagct cttttgtatt
     5761 tgttgtattt tcgtatttat tgtattattt aataatgtct attagttctc cattttgtaa
     5821 agttttaggt gtaattacta tactgaactc tttttgcaaa tcttttataa tatctaattc
     5881 tttttttgtt attattgaga ttgtgttacc acttcgattt cctctagaag ttcgaccaca
     5941 tctatgcagg tattcatttt tactttttgg aaaatccaag ttaaaaacat gacttacatc
     6001 cacaatatct agtccacgag cagataaatc agttgtaatt aaaatctttg ctttaccaag
     6061 tttaaattta tttattgcat tctttctatc ttctttttcc atatttccaa aaattcctat
     6121 tgctttgtaa ttatgataat ttagttttga tactaggact tctatatttt tttcattatt
     6181 tacaaataca atagcttttt ttggatttgt agctgctaat gcctttctaa gaaaagtaaa
     6241 tttatccctt atctcaccta ataaatatga gtgattgatg ttaggattta tttgtgattt
     6301 ttcctttgtt tttattattt caaactcctt cataagttta tcacaaattt ttatagtgct
     6361 atcagtaaga cttgcagagc atcctataat ttgtctatct cttaacgttg ttcttattat
     6421 gtcttctata caagttatat tttttccatt caatagattg tccacttcat ctaagactat
     6481 agtttttata ttatgtgatt ttagcttctt ttgttttatc aagtctaaaa ctcttccaca
     6541 agagccaatt actatgtgtg gttttactgc ttttatattt tttatttgtt tttgtatatt
     6601 gacctcccca attaaagcta aagaagtaac ggagagttct gcatttttag ccaataattc
     6661 aacctggttt gtaatctgca ttactaattc atgcgtaggt gcaagtataa gagcttgtgt
     6721 ttctcttttt gatgtatcaa ttttttcaaa tataggtaaa aggtacgcta gtgtttttcc
     6781 tgttccagtc tgtgaattaa tcaataaatc cttattttga attatattcc ctataacaag
     6841 tgactgaact tctgttggag aagttatgtc ttgtttttta agtccatcta ttagagtgga
     6901 acttattttt aattgttcaa atgtattcat attttgctcc ttatatttaa taattatagt
     6961 caactatttt ttattataca ttgtttattc aaatcgaact atatattctt ttataattat
     7021 gtgtaatttg aaagtaaata tcaattatat ttataccttt atatttctga tacctttgca
     7081 ttttatagat ttctaaaata aacaaataaa agttgtaatt gtttgatatt tgttatatat
     7141 tatatataac aaatatcaaa actataatat ggagggaaat tatgttaaaa gtaatcaatg
     7201 tatctaaatc atataaaaat aaaaaagtag taagtagtgt ctcatttgat gtaaatgatg
     7261 gagagctttt tgggtttata ggacataatg gagcaggtaa aactactact attaaagcaa
     7321 ttgttggaat acacgatttt gaagagggag aagtactaat aaattctaaa tctataaaaa
     7381 aacagcctgt tgaatgtaaa aaggaaatgg catatatacc tgataatcca gatttatacg
     7441 agtcattaac gggtatacaa taccttaatt ttatagctga catatttgaa gttgccaaag
     7501 acaaaagaga agagttaata aaatattatt caaataagtt tgaactcaat aaagcattag
     7561 gagatttaat atcttcatat tcgcatggta tgaaacaaaa attagctata atttcggctt
     7621 tgatacacag cccaaaaatc ttaattcttg atgaaccttt tgtaggtctt gacccaaaag
     7681 cttcctttat attgaaggaa attatgaggg agttttgtga caaaggtggt tgtatatttt
     7741 tctcaacaca cgttttagaa gttgctgaaa aaatttgtga taagattgcc ataataaaag
     7801 atggaaatat aattgcttat ggaactacag aagaagttaa gggaagtaat tcacttgaaa
     7861 gtatctttat ggaattaata gagaaataaa atgtttttta tacaatatag tgtctaaagt
     7921 gaggaatgat aaaatatgag caatctaagt atattaataa aaactaatat aataaatgaa
     7981 tttaaattaa atgcgttaaa aaaagctgat agtagagaaa aacaaaaaat aatggggatg
     8041 atactttcag ttgttattat aacagggtta ttgatttttt atgtaactag tataagtctt
     8101 tctttagttg atattttaaa gcaaataaat cagatggaaa tgctacttat atttggattt
     8161 ggattatcaa cattaacaac tattgttaca tcattatata aaacgtcatc atatctgttt
     8221 caagcaaaag atttagacct tttgactagt ttacctataa aagaatctac tgtactagca
     8281 agtaagatat taatgttggt tggagcaaac tatttatttt cttcaatatg tatgattata
     8341 ccagcagtgg tttatttttt taattctgat gtaagtataa cgtatttccc ttatttgata
     8401 ctgctatttt tagctttacc attattacca atagttgctt catcaataat ttttatgttt
     8461 ataggacaga tatcatcaag aataaaatac aaaaatttaa tattaattat aggtagtatt
     8521 atattgatgt tgctttatac tctacttatg acaagactta actctatgtc ggttgagatg
     8581 ttaaagaata gtgtatctat ttcagagacg attagaaaaa tatatccacc tatatattat
     8641 tttgttgacg ctcttaaaac aggaagtgta gtttcctttt tagcatttat agcaatttca
     8701 ataataccat tttttatgtt tgtaacatta tttgcaaaaa aatttaaaga tataaatgct
     8761 aagatgagtg aaggatataa gtcaaaaact tataaattta agagccaaaa aacttcaaga
     8821 ccttcaaaag ctctattaaa aaaagaattt aagagatata cttcttcatt tatatacttt
     8881 ttaaattcat ctgcaggttt gttattccta ataataggta caattttaat attattattt
     8941 ggagcagata aagtgggtga gttactgaaa ttgaatatag attttaattt tataaaaata
     9001 caattattgg gagtattctt ctttataata gtaatgaatt gtacaacata ctgttcaatt
     9061 tctcttgaag gaaaaaattt atggatactg aaatcttctc caatagatga aagagaaata
     9121 ttctggtcaa aaatattgat gaatttttta ttgaatgcac cattatctat tttatgttta
     9181 atattagtat ctataagatt aggttttgaa attcagttta ttattgtatc aatctgtttg
     9241 attgcaagtt tttcaatgtt tgtggcattg atgggattat tatcaaattt attatatcca
     9301 aaccttgatt ggaaaaatga gatggctgta gtaaaaagaa gtgcaagtat gataattaca
     9361 atgttagtat cattagctta cgtaattata cttggaggta tatatgtatt acttggaatt
     9421 tcgtttttaa atatatatat tattttggca agtatactta cttttatatt agattttatg
     9481 atatggagaa ttatttctac taaaggagta agaatattta aaaatttata ggtgtattta
     9541 tgtcaaataa agacttatct tttttagtct tgcaaataat aggttaa
//
LOCUS       DACTGW010000148         9582 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.148, whole genome shotgun sequence.
ACCESSION   DACTGW010000148 DACTGW010000000
VERSION     DACTGW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.148"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..464)
                     /locus_tag="I9573_002646"
     CDS             complement(<1..464)
                     /locus_tag="I9573_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HAT4797256.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALS"
     gene            685..1509
                     /gene="pdaA"
                     /locus_tag="I9573_002647"
     CDS             685..1509
                     /gene="pdaA"
                     /locus_tag="I9573_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HAT4797257.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            1698..2462
                     /locus_tag="I9573_002648"
     CDS             1698..2462
                     /locus_tag="I9573_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4797258.1"
                     /translation="MTTICVMIDHIFLDTEYRQPTIHKHLAKHIIISQSGNLSVTFDK
                     GESLQCKGIIIDSNVSHTVSSKDNKMLVFLIDDTSILAQNISQHQLLNHSFFVIDNLI
                     VERIQNIYTACQVKGLSQSYIDFSKQAFRLLNLNYHISSITDSRIQWALNHILKSTHI
                     HNEIFFEICAEVKLSKSRFSHLFKEQMGVSLNSYLALSKLRKAYELLLKSGDITSAAM
                     DAGFSSPSHFSASSKKYLGITASELIGKCKIYFIRQ"
     gene            2565..3743
                     /locus_tag="I9573_002649"
     CDS             2565..3743
                     /locus_tag="I9573_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT4797259.1"
                     /translation="MKTNKISFNKGLYYLNTNANFNFQLNRIIMWDGGRLEDIQKVSS
                     KIVDSESWKTELMYLAQEAEQDGRTENAIAYYRMSEFFMYDGDPDKISVYKKATTLFY
                     NYYSEYFSNGTVQIFHVPYENITLPVMYAKAQGEVKDVILLHGGNDSYFEEFFFPMLY
                     LASQGFDIYLFEGPGQGGVMRLQGKHFTYQWERPVKTILDYFHLENITIVGASLGGFL
                     APRAAAFEKRIERVVAWSVFPNFLDIIVGSQPIKMQNLLRLLLKLKTKWLVNFIIKQK
                     MKSGDSMVVWGLKHGMYAYEASTPYEYFQKIKYYQIDSIAKNINQDILILGATQDHFI
                     DYRTVGKEIDLLNNVRSLTFRLMTEAEQASNHCNCGNSKLVLDTISNWIMMMKIHDRV
                     "
     gene            complement(4114..4899)
                     /locus_tag="I9573_002650"
     CDS             complement(4114..4899)
                     /locus_tag="I9573_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4797260.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFDIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            5364..5729
                     /locus_tag="I9573_002651"
     CDS             5364..5729
                     /locus_tag="I9573_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4797261.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            5807..6361
                     /locus_tag="I9573_002652"
     CDS             5807..6361
                     /locus_tag="I9573_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAT4797262.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            6581..7255
                     /locus_tag="I9573_002653"
     CDS             6581..7255
                     /locus_tag="I9573_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAT4797263.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      7610..7706
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8124..8363)
                     /locus_tag="I9573_002654"
     CDS             complement(8124..8363)
                     /locus_tag="I9573_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797264.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(8508..9317)
                     /locus_tag="I9573_002655"
     CDS             complement(8508..9317)
                     /locus_tag="I9573_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT4797265.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT         3450 a         1413 c         1182 g         3537 t
ORIGIN      
        1 tctgataaag ctctattttc cactttagaa ttacaatttg ttaaagtatt ttttaagtat
       61 tctaagctat aatctttatg atatggttct aaaataactg ctaattcaat taaatcaata
      121 aatttgtttt ttaactttgg catatgatac tcaaaaaact tttttttcag acttaaatca
      181 tgacatataa tgaaactatc ttctacaaaa tttacaagtc tatcttccac cagttctatt
      241 tcattagcta tttctaaatc atttttatct aaatttttac atactgagaa tacttctata
      301 ggtacctcaa atttgttttt tattaatgat tcataggtat atattttcca gtctttaacc
      361 ttgacagctc caacttctaa aatttctgaa tttaaacaat ctaatccact aacttctata
      421 tctaaaaaaa caacatcatt caatacactt attatattat ccatttttat atcccttcaa
      481 gttaaaccag cctaacttat ttacaatcat ttctctttat attttatcat ttatatacac
      541 tttatttaag ataattaata tcaactttat atcttacagt aaacttcttt catttattat
      601 ttgtatatgt gaatattttt aactataaat attcaaacta tattataaga aatatacttt
      661 aaattttaaa taagaggtga aattgtgaaa aaagatagcc taaaaaaata cattatgata
      721 ggagcatttg ctctaatact ttttggaatt gctagtataa attttaaatc attagataaa
      781 acaaaaactc aaatttctag tccaacactc gacacacatg aatatgattg gtattttaac
      841 cctagggagg atggtaaaca gccatcacct ataaaagaag ctgatttttt taaaaaatac
      901 ggagcttatt atgtaggaaa tcccaatgaa aaagttatat atctatcatt tgatgctgga
      961 tatgaaagtg gaaacactcc aaaattatta gatacgctta aaaaacacaa tgctaaagct
     1021 caatttttcg tagttgaaag ttatataaaa agcaatccag agttaataaa gcgtatggaa
     1081 aaagaaggac atttagtttg taatcattca aaaagccatc catcaatggc aggtattaca
     1141 gattttgaaa aattcaaaga agaaattaca agtgtagaaa aagcatacaa agatgttact
     1201 ggaaaagaaa tgcctaagta ttttagacct ccaatgggta aatttagcga acaatcttta
     1261 aaatatacac aagatttagg atataaatca atattttgga gttttgctta tgtagattgg
     1321 tatgaaaaga aacaacctac tcatgaattt gccaaaaaca aaatttattc aagaactcat
     1381 cctggtgcaa tagttttact gcatccaaat tcatcaacaa atactgagat attagatgag
     1441 gttcttacac attgggaaaa agaaggttat aaacttaaaa cacttgatta tttaaataat
     1501 aaaaaatagt atcataaatt ttaaaaaact ctatcatgaa tcatagtaaa aatatagtta
     1561 tttataaata ctttaattct cgatagagtt ttttattttt cattgcctaa aataggattt
     1621 ttaaatactt gtaatcacta agctttcttg ttaaaaactg tataatatgc taagctatga
     1681 acaaggagtt gacagctatg actaccattt gtgtaatgat tgaccatata tttttagata
     1741 cagagtatag acaaccaact atccataaac atcttgcaaa acatattatt atatcacagt
     1801 ctggtaactt atctgtgacg tttgataagg gcgaatcatt acaatgtaaa ggaattatta
     1861 ttgattccaa tgtatcgcat acggtgtcaa gtaaagataa taaaatgttg gttttcttga
     1921 tagatgatac aagcatactc gcccaaaata ttagtcaaca ccaattacta aatcactctt
     1981 ttttcgttat agataatctt atagttgaac gaatacaaaa tatttataca gcttgtcagg
     2041 taaaaggatt gtcccaaagc tatattgatt tttcaaaaca agctttccgt ctattgaatt
     2101 taaattatca tatttcgtct attacagaca gtagaataca atgggcattg aatcatattc
     2161 taaagtccac acacattcac aatgaaatct tttttgagat atgtgccgaa gtaaaattgt
     2221 ccaaaagccg gttttctcat cttttcaaag aacaaatggg agtatcacta aacagctatt
     2281 tggctttatc aaaacttcgc aaggcttatg agttattgtt aaaaagtgga gatattacct
     2341 cagctgctat ggacgcaggg tttagttcac cgtctcattt ctcagcttcc agcaaaaaat
     2401 atcttggcat tactgcaagt gagcttatag gaaaatgtaa aatatatttt atccgccaat
     2461 agcgtctatt tgaaagtcag tacttctaat atttgttaca ataaatgcat agcattaaag
     2521 attactcttt attgctatgc attatctttt taggagtgat gacattgaaa acaaataaaa
     2581 tttcttttaa taaaggtcta tattacctta atacaaatgc caattttaat tttcagttaa
     2641 acaggattat tatgtgggat ggggggcgct tggaagatat acaaaaagtt tcctctaaaa
     2701 ttgtcgatag cgaaagttgg aaaacagaac ttatgtatct ggcacaagaa gctgaacaag
     2761 atggacgcac agaaaatgca attgcttact atcgtatgag tgaatttttt atgtatgatg
     2821 gtgaccctga taaaatatca gtctacaaaa aagcaactac tctattctac aactactact
     2881 ctgaatattt ctcaaatgga actgtacaaa tttttcatgt accatatgaa aatatcaccc
     2941 tacctgtaat gtatgcaaaa gcgcaaggcg aggtaaaaga tgttatattg ctccacggtg
     3001 gaaatgattc ttactttgaa gaattctttt tcccaatgct ttatcttgcg tcacagggat
     3061 ttgatattta tctttttgag ggacctggtc aaggtggagt gatgcgtttg cagggaaaac
     3121 actttactta tcaatgggaa cgtcccgtta agacaatttt agattatttt catttagaga
     3181 atataactat tgtaggtgct tccttgggag gttttcttgc tccacgagca gcagccttcg
     3241 aaaaacgaat tgaacgcgtg gtagcttggt ctgtattccc caatttctta gacataatag
     3301 ttggttcaca accaataaaa atgcaaaatc tacttagact actgctaaaa ctcaaaacaa
     3361 aatggctagt caattttata ataaagcaaa aaatgaaaag tggcgatagt atggtggtat
     3421 ggggtttaaa acatggaatg tacgcatatg aagcgtctac accctatgaa tattttcaga
     3481 aaatcaagta ttatcaaata gattctattg caaaaaatat aaaccaagac attttgattt
     3541 taggtgcaac acaagatcat tttattgatt atagaactgt tggtaaagag attgatttac
     3601 tcaacaatgt ccgatccctt acatttaggc ttatgacaga agcagaacaa gctagtaacc
     3661 actgtaactg tggaaatagc aaactggttt tagatacaat ttcaaattgg attatgatga
     3721 tgaaaataca tgatagagtc taaatattga tttttaaatt atacaaaatc catctgctag
     3781 catatcctta ttgctttgtt caaataatca cttgttaaaa aattggttcc cacttacgaa
     3841 atactacgtt tatagaaatg aactatatca tgtaagcaca caatatatat gataataaga
     3901 cccaattact caatgtaact gggtctttct tatgtcatag attttatagg actcatgaaa
     3961 tgtaaaagag tatttcaccg atttccaaag ggaactttac ttagataagg agaccataaa
     4021 tgctaacaaa cccatctact agacaccttt caagtatttg agcgattata caatacacgt
     4081 taagaattac acgaaaattt attattttta tagctattta aaaagaattg tccattcatt
     4141 tatctctttg tatcctatat tatgatatat tttacctgct acatcattat tataaaaaag
     4201 acatggaact tttccttcca acaataattc tttactcaaa ttgtaaacca tataacttgc
     4261 aagtcctttt cccctatatc ttttatctgt acttacagag accaccattg ccaaaaaact
     4321 agtttccata ccagttgaca cagtgctaat catagtatca tcatttctaa taaaatatgc
     4381 tcttacattt ccctctttta aatgttcttt cctttcttct atgtaatttt tacttacttt
     4441 atagtctgac ctatttaaaa gttttcctat ttcatcaata tctttttcct gagcattttc
     4501 tattttatac tccttcatgt tggaaaaatc tatttctttt atttctttta atacacaaaa
     4561 tttattatct agtttttcat aaaaatcttt gtatttactt attagtctat ctattacaca
     4621 tttttttcca cttatgcttt ctacatctaa atcttttata tgattcttca tttctcctat
     4681 atcaaaatta tttttaaaac tataaattat aagtgttgac ttatttttta atattataga
     4741 ggttatattt ccatcttcta cctgagacca tatatcttga tattgagagc taaatccata
     4801 attctcaacg tatccaatta tgaataaatt taaactttct tcatgtgata catattgcca
     4861 aaattctttt tctaaagtat tatctatttt tacaatcaag cagatatcct ccttctatgt
     4921 taataatgat ataccttata atttatagta tcatttaaaa tcattatatt ctaccaaata
     4981 ttctaaattt ttaaatatct atgcaatatg ttcgtattta tttttatatt ctgttactgc
     5041 ttcactccaa gcttcttcta ctgaaatacc tctcttattt gcaatttttt gtgctaattc
     5101 tattattttc atagcttttc ttgatatatt catatatatt cctcctctaa attatcataa
     5161 gttattttaa agttaaattt ctatagtcat gttatatatt tatttactta tatcttacta
     5221 attatttatg tactaaatat gaactacaat agaagaaatt tagaacttaa taaaacataa
     5281 aatttatata cagttcatat atatttgtca tatcacaaga caagtgatat aatttattat
     5341 cattttactg ggggtgatat gctatgaaaa taagagctga atatacatgt cctcttgaaa
     5401 ttgtacatga cataatcaaa ggaaaatgga aaactatcct tatatttgaa ttaagacaat
     5461 ctaataaaac attttctgaa ttgcaacata atatttatgg aataaatcaa aaaatgttga
     5521 ttcaacaatt aaaagagtta cgtgattttg ggattattga taaaataagt tctgctggat
     5581 atccattaca tgttgaatat tttcttacta atcgtggtaa aaaaatgtta aaagcagtgg
     5641 aaataatgca agaaatagga atcgaatata tgcttgaaca tggtcaacaa gaaattctag
     5701 ataataaagg aatttgctac aataagtaat cctatacata ccaaaaagta agtaatttac
     5761 attttttaca atagtattat aataaaagta aatagaaggt ataatcatgt ctatagcttt
     5821 aatattgatt gatattcaaa atgattattt taaaaatgga aaatgtgaat tatttcaatc
     5881 agaagaaact gctgaaaatg ctaaaaaaat actactgttt tttagaaaaa acaatttacc
     5941 agttattcat atacagcata tatcaacaga taaaacagct tctttttttc ttcctaatac
     6001 tcatggttca gaaataaata aaattgtttt tccactagat aatgaagaaa ttataattaa
     6061 acacacacca gatagtttct ttgaaacaga tttgcaaagt atccttgaga aaaaaaatat
     6121 tactaatcta gtaatctgtg gaatgatgag ccatatgtgt attgatacaa gtgtaagaac
     6181 agcaaagaaa cttcgatacg acataactct aataagcgat gcatgtacaa cgaagaattt
     6241 atcttggaat aatatagaaa ttaatgcaaa tactgtacat caagtattca tggcatcact
     6301 acagaacagt tttgcagatg taaaaaacac agataaattt ttatctaact atcacaaata
     6361 aaaacataca ctatgttaaa agagcaagtt tgatttattt aagcttgctc tttaatcatt
     6421 taaaattaga tgctttctat catttttata tacataatat acgtttatac attttatatt
     6481 catatataac tattatataa attttaaact ttactatatt cacataaaaa atgtagtatt
     6541 atagtatata taatattata tagaaggggg tgctagataa atgaaccatc atatattgat
     6601 tcaatttgaa tatatagcaa gaatcattac tgctggtttc tgtggggctt taattggata
     6661 tgagagaaag aatagactaa aagaagctgg aatacgcact cattttatag tggcattagc
     6721 ttcagcactt ataatcattg tttccaagta tggatttaat gatatagtgg gtactccagg
     6781 tattggtcta gacccagccc gcgttgcagc acaagttgtc agtggtatag gttttttagg
     6841 tgctggatta atatttatta gaaatcaatc agtaagtggt cttactacag ctgctggtat
     6901 atgggcaaca gccggtacag gtcttgccat tggctctgga ttatacttag ttggtattgt
     6961 ttcaagtatc attattgtta ttgtacagat aacacttcat agagatagga agtggatgaa
     7021 actccctaca gcagagcaag tttctcttaa aatttctaac agcactgatt ccatagaata
     7081 tattgaaggt aaatttaaaa aatctaatat tgaaattata aacatgaata ttaaacgcct
     7141 tgatggggat tggttagaag tagttatata tactaagtta cctaaaggat ataaaaaaac
     7201 aaatttatta aaattattta gcgataatcc tcttatagaa actttagaaa tataatataa
     7261 aaattgaagc ttaaaaataa taatatattt aatcaaggtc aatatataaa taattacggg
     7321 atgagtctat ataaaattca tcccttgttt tttgccattc atatatattt tttcaagata
     7381 tttttgctca tctattatct agccaagtaa ctcttgtgaa ctaaactttt taatacaaaa
     7441 acttcaatac aaaactaaat ataattttta ttaaatattc tacatgtata gtttgtttct
     7501 tgagtgaaaa atagatttca tattgttaag atactgttaa atatttcaat tttctgacaa
     7561 gtatgttgaa caataactta tcctagtgta ttattttagt tgtagaataa aactaaatcg
     7621 gcaaaactag agaaatttag tgacgcaaag ctatagggac taacgcttat aaaataaact
     7681 atgagttatg tcagccagtt gccaaaaaga tattgttctt tttgttttat gaaagttttt
     7741 tagggggata gtaatatata tgtttagaga tgaaatggat aaatgtacac acatgttaac
     7801 tgcttatatt agtagtttat atgattattg tgattttata gatacacagc tagatgattt
     7861 tatactagag tacggagaaa atgtagtaga atcttgttta catcaagtga tggtattggt
     7921 aagtaagtat aattaaatag aaattgtttt ctagagaacc ctttttagtt ttatctacat
     7981 ttaaaaatgt tattatatta aaaaactaat ctcccctata tgcaaagttt tcttccatat
     8041 aacaatttct tcccatttat actttttcac ttgtatcttt aataaaattc aacttcaaat
     8101 attaatatac ttcaaaaatt attctatttc ttatctttac accaattcct atcagtatta
     8161 tcctttatat aggttctaac tatttcgcca caatctaaac ataaatctga atatatagta
     8221 gattgtactg gaaaagtttt atttataact ttatctaatt ttccatctcc tctatatttt
     8281 aatccaatag tttcagcata tatgccacca gttgttaaaa tacccttttc aatatttaca
     8341 cttccacaat tagaacattt cacatttata accccctaaa caattatttt ttcatgctat
     8401 cttataattt aaaatataat ttgctaccga ttttataaca cagtagcaaa tcggtggcaa
     8461 atcaacattt tttcattcta tttaccatct aatccattga aatatggcta tttagttata
     8521 ggaacttgaa tttccgttac aaactcttct acaattccag tctcatgtat atctatctta
     8581 tagatttcta tagggtcagc aattatctta taattatttt cttctataaa tttaaacacc
     8641 ttctccatat acatcttatt attagaatta ctcccagtat aatttaaagt tacataatat
     8701 ccttcatcaa aaactatatt atatactttc tcattacttt ccagaaaaca aaatacagat
     8761 ttaaactcat taaatatacc tttattaatt gcttcagtag aaaaggcaga accaatattg
     8821 ttgtttccaa gtatattaaa tctatcttca tattctttct gaagtttctg aattaaaaaa
     8881 tcgaagtctt catccctctt tatatcagca tttaattcta aagcttttct tttttctata
     8941 tatacaacct ctatctcatc tatttttgta ttttgaatta cactttcaat cgaactcaat
     9001 ctttttataa tattttcctt atggcttata aattcagcta tttttttatc aattaaatcg
     9061 agttcttcat ttaaaatatt tttcgtagac tctatacttc tgtcatctag gtattctttt
     9121 attttcttca ttggaaagtt caagcttcta aattctttta ttaaatttaa tttccatata
     9181 tctgatatat tatacattct atatccattt atatctctaa ctggtcttag aatgcctatt
     9241 tcttcataat acattaatga atctctacct attccatata ttttagatat ctctcctatt
     9301 ttatagtaat ctttcattta tataataaac tcctccgaaa tcctattttt attttaaatc
     9361 aacattatta ctctataata ctataatatt agaatatact aaaaaaatct ttaaaacaag
     9421 gataggaaaa taaacatcct aataattaat tattaaaata tttttatatt gtaatgttag
     9481 gtatagtatt tataacttca acttttttat aaatatattt tagttttata tagttttatg
     9541 tttacagcta ttatatccat ctctatgtaa tatttttcat at
//
LOCUS       DACTGW010000149         9577 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.149, whole genome shotgun sequence.
ACCESSION   DACTGW010000149 DACTGW010000000
VERSION     DACTGW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.149"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            95..1498
                     /locus_tag="I9573_002656"
     CDS             95..1498
                     /locus_tag="I9573_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT4797266.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFMLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     TSLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     gene            1872..2570
                     /locus_tag="I9573_002657"
     CDS             1872..2570
                     /locus_tag="I9573_002657"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAT4797267.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILKGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2593..3594
                     /gene="trpS"
                     /locus_tag="I9573_002658"
     CDS             2593..3594
                     /gene="trpS"
                     /locus_tag="I9573_002658"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAT4797268.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANAIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4246..6876)
                     /gene="cdtB"
                     /locus_tag="I9573_002659"
     CDS             complement(4246..6876)
                     /gene="cdtB"
                     /locus_tag="I9573_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094607992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin binding subunit
                     CdtB"
                     /protein_id="HAT4797269.1"
                     /translation="MKIQMRNKKVLSFLTLTAIVSQALVYPVYAQTSTSNHSNKKKEI
                     VNEDILPNNGLMGYYFTDEHFKDLKLMAPIKDGNLKFEEKKVDKLLDKDKSDVKSIRW
                     TGRIIPSKDGEYTLSTDRDDVLMQVNTESTISNTLKVNMKKGKEYKVRIELQDKNLGS
                     IDNLSSPNLYWELDGMKKIIPEENLFLRDYSNIEKDDPFIPNNNFFDPKLMSDWEDED
                     LDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEK
                     ASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTES
                     NTAGVSVNVGYQNGFTANVTTNYSHTTDNSTAVQDSNGESWNTGLSINKGESAYINAN
                     VRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLSPGDTYPKKGLSPLALN
                     TMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYIS
                     QIDSISASIILDTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYF
                     NDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDD
                     YNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTSN
                     SIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITE
                     LNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAPTQTNKEAL
                     DYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYK
                     KLRIYAITPDDRELLVLSVD"
     gene            complement(6929..8320)
                     /gene="cdtA"
                     /locus_tag="I9573_002660"
     CDS             complement(6929..8320)
                     /gene="cdtA"
                     /locus_tag="I9573_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin enzymatic subunit
                     CdtA"
                     /protein_id="HAT4797270.1"
                     /translation="MKKFRKHKRISNCISILLILYLTLGGLLPNNIYAQDLQSYSEKV
                     CNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKY
                     SQTRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTEN
                     QNEISLEKFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLP
                     YTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKA
                     NYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKR
                     EPIPTNLTVYRRSGPQEFGLTLTSPEYDFNKLENIDAFKSKWEGQALSYPNFISTSIG
                     SVNMSAFAKRKIVLRITIPKGSPGAYLSAIPGYAGEYEVLLNHGSKFKINKIDSYKDG
                     TITKLIVDATLIP"
     gene            complement(8710..9456)
                     /gene="cdtR"
                     /locus_tag="I9573_002661"
     CDS             complement(8710..9456)
                     /gene="cdtR"
                     /locus_tag="I9573_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HAT4797271.1"
                     /translation="MDILIFDNDVCFGIKLKEKINNILIKEGFDNDVIRLYYNANLLL
                     KELTEKNKVKIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYVKEKNYRSNFFTIYSDSSLWKISF
                     DEVIYFETSAIPHKIKLVTTSRIFEFYKSLRSLSDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         3293 a         1266 c         1385 g         3633 t
ORIGIN      
        1 ttttgaaaaa tatcagttta ataataatga atctatttta ttaattttga aattaaattt
       61 aaaataatta atagagaggg gaaatgaaat taggatggaa aagaaaaaaa taggcctatg
      121 gagtttggta tttatgaatg tatcagcttt atatggtata agatggatag ctaagtctac
      181 agcatcaagc tttggtttag gattaggtgc aatacctatg tggttattat tctcatttat
      241 atactttgta ccaggagctt taatttgcgc tgaattagct tctacatatc aaaagggtga
      301 tggaggtctt tacgattggg ttaaggaagc ctttggagaa aagtatggat ttttagtttc
      361 ttggttaaac tggacagcta aaatattttg gtattcttca tttttaactt tcttagcagt
      421 aaatgtttct tatacaatag gaaatccagc tcttgctgat aataagatgt ttgtacttat
      481 attatcttta ataatattct gggcattatc tcttatagca actaaaggaa taagttttac
      541 aaaaatattt actaatacag gtgcattagg ttcaacaata ccaagtgcta tacttataat
      601 attttcattt gtagcagtat ttatgttgaa aaaacatgat atagcttcta catatacaat
      661 agcttcaatg actccaaaac ttaatatgga tgcatttgtt gctatatcag ctataatgtt
      721 tggtttagca ggagcagaga ctgctgctaa cttcataaca gaaatagata agcctgaaaa
      781 gaacttccct aaggcaatat taatatcagc tggaatagta gcttcattat atgtattagg
      841 ttcaattgca ataacaatga taataccacc agaccaaatt actgcttcta aaggtgtatt
      901 agatgcactt tctgctgttt gtacgtcttt aggaattggt tcttggttgg ttcaattaat
      961 agcttttggt atagctttct cagtattagg agcaatagtt ctttatatag catcaccaat
     1021 aaaaatgtta tttggtagtg ttaaaaaagg aatatttcca gatagtttgg tagaggtaaa
     1081 tgaacacaag ataccttcta aagcagttat acttcaagca ataatagtta ctataatact
     1141 attggtgact acattaatgc cttctgttga tgctatatac aatgttcttg taactatgac
     1201 agcattaact gcattattcc catatgtatt attatatgct tcttatatta aactaagaaa
     1261 agagagacca gatgaaatta gaccttatac tatggcaaaa tcaacaagta cttgcctagg
     1321 tttagcaaaa atggttttag ttgtaactgt agttggtata ttattatctg cagcaccagt
     1381 tatgccgaca tttgctgaaa atgttatata tgagatagag atgattggtg gaggtcttct
     1441 agttatatta tctggtctat ggttatggaa tagatatgaa aagaaaacag ctaaataaaa
     1501 tgtattttaa aaatttcttt tggatattat aatatatagt tgacaattta tagtgtaagg
     1561 aatattataa aataaagaaa ttaataaatt aaatcctgag ataagaaata gtaaataaat
     1621 atatttttgc acagagaatt aaggatgctg agaacttaat aaaaagtatt tattgaatgg
     1681 gtctttgagg agtaaaatga aatgtaattt aagattacag agtatttgat atcgtgtatg
     1741 cacgttatag catatattaa gatatatata ttcagttttg agtgtatata aatttaggtg
     1801 gtaccacgga aaatatatcc gtcctattga tttaggacgg attttttaat atagaaaatt
     1861 taggaggaaa aatgaacgta ataggaatta taggtgcaat ggatgaagaa gttagtattt
     1921 tagttgattt aatggatatt agagagacta taaaaaaagc tagcttagaa ttttataagg
     1981 gaattttgaa aggtaagaat gtagtgttag taaagtgtgg tataggtaag gttaactctg
     2041 ctctttgtgc ccagatacta ataagcgaat ttaaggttga tgctattgtt aatacaggtg
     2101 ttgcaggagc tttaaatgaa aaacttgatg ttaatgatat agtaatatca actgatgcta
     2161 tacagtatga tgtagatact acagcatttg gagaccctaa gggagttata ccaagaatga
     2221 agacttctgt atttaaagca gatgaaagac ttatagatgc tgcttacaaa tcttctgtgg
     2281 aagaagtaaa gactcataaa gtattaaaag gaagagtagt tacaggagat aaatttataa
     2341 attctaaaga attaaaagaa gaattagtaa atgattttgg tggatattgt ggagaaatgg
     2401 aaggtggagc tatagctcat gtatgctatt taaataatac tccattcgta ataataagag
     2461 caatgtctga taaggcagat ggaagtgctg atgttactta tgatgtattt gtacatgatg
     2521 ctgccaataa ctctaaagac atagttttaa atatgttaaa atcaatataa atatataagg
     2581 aggtacaaaa aaatgagtga aaaaaaagtt atatttagtg gagcacagcc atcaggcaaa
     2641 atgactttag gaaattattt aggagctata aaaaactgga ctgaacttca agataattat
     2701 gattgctatt atagtatagt tgatttacat gcaataacag tacctcagga ccctaaagtt
     2761 ttaagagcga atgctataga attacttgct caatatatag cttgtggatt agacccagaa
     2821 aaaaatacta tatttataca atcacatgtc aaagagcatg ttgaacttat gtgggtattg
     2881 aatactatga cttatatggg tgaattaagt agaatgactc aattcaaaga taaatcacaa
     2941 aagagtgaag caaatctaaa tgcaggttta ttcacatatc cagttcttat ggccgctgac
     3001 atattgttgt atcaaactga cctagttcca gttggagatg accaaaaaca acatttagaa
     3061 ttagctcgtg atttagcaaa taggtttaat aatagatttt cacctacttt tgtagttcca
     3121 gaaggatact acccaaaagg tggagcaaga gtgatgagtt tgcaagagcc aactaaaaaa
     3181 atgagtaaat ccgattcaaa tgaaaatgca tttatactat tagcagatga ttcagattct
     3241 ataaagagaa agataaaaag aagtgttact gattctttag gtgttgtaaa atacaatgat
     3301 gaacaaccag gtattaaaaa tctaattgat atatattcta atttaagtaa aaagagtgta
     3361 gaagagattg taaacatgta tgaaggaaag ggttatggca tatttaaaga agatgtggct
     3421 gaagtagtat cagaagcttt aagacctata agagaaaaat atgttgacct tcttaataat
     3481 aaagattatt tagaaaaaat atatagtatg ggagcagaaa aagccgaaaa acaagctaga
     3541 aaaactctta gaaaagttta taaaaaagtt ggatttatag agagaagata ctaattatta
     3601 gctatcttta cttgataaat aaattttaaa gttattaaaa aggtatatga tatgaaagag
     3661 agagtctcaa agtgaacttt tgttcatgag acactctttt ttgtatgaaa taaaatatat
     3721 tttaatcact tcaacaatga aaaagaggat tatctctatt ttgagacagc atcttttgat
     3781 aataaaaaaa gatgagcttt tgctcatctc aaaggggtta gattattctt actttgggga
     3841 aagtagaata aaggtaaaag ttaactgatg tcaactttgt cttttttgca tatcactact
     3901 gagcataaaa aaatcagaaa accatagcct tctgaaacaa cttctaacat acattgtatt
     3961 ttatacccaa tatgtaattt atgagttaat tttaactctt ataatggaaa ttgtcaataa
     4021 tatatcaaaa aaatattttt taaatttata taattaaatg aatttatgtt tatatatagg
     4081 tgatatgata cagataatta tctttataat acaatatagt tctaaaaaag aatatgttag
     4141 aagaagagaa tatttaaata aaaaatacct tataaagtta attttttagt actttataag
     4201 gtaaatatat attgtattgt ggggacaaat ttaaatcatt tgatactaat caacactaag
     4261 aactaataac tctctatcgt ctggagtaat tgcatatatt cttaatttct tgtatgtcat
     4321 aatattttct ccacctgtaa aataactcct aagattaaca taattagttt taggctgatt
     4381 tggatctcct aaataattac gcatttcttt atctttagtt ccaatatctt taaatccaga
     4441 atattgtaaa gtagcttcaa ctctatattt ttggatataa tcgagagctt ctttattagt
     4501 ttgtgttggt gcaaaataca taccatttat ataagtattt cctgtacttg gattaaaatt
     4561 taaatctgca aaatatattt tatgtgcatc cattatttct tggtcagttg gaattttaac
     4621 ttctggttca tcaagtattt caggagtact atttagctct gtaatagata agttatctaa
     4681 gtatgttgta ccactaccaa ttaatgttat ctctatatta gaagaatctt tttcagtagt
     4741 ttcaaattca taactaaatt ttgtatatcc ttgttctggt accaaataat ccgttttctc
     4801 ttcttttgct tttatcttta caattattga attagatgtt aaaggatcct ttgaatatcc
     4861 actaaaaaca taacgtttat aaggttttag ctcagacata gggattttaa tcttcgtctc
     4921 accatttaat ttatttgctg agccttgtaa accatcttga ttcgtagtat tgacattact
     4981 ccatgtacta gggtaattat tataatcatc aaaattagta aagtaagttg gtgtttttat
     5041 aagtatattc attcctcttt caagtttaac attatatatc tttttatcag acaaagtttt
     5101 taaactatct ttaatcttat tggctgtatt atcatcaaat atgagttcaa cacaactttc
     5161 atctattggt atatcattaa aatataacaa accatctttt ttagtagcgc caaaagcttt
     5221 ttcaattgct tctccaattg taagttcagg tgttttatct tctggatcct gtaaattttt
     5281 agcagtaact cttctttcgt aagattcatt ctctgtatct aatataatag atgcagaaat
     5341 actgtcaatt tgacttatat agtctgacca actatttcct tctgttacta tttgtccaga
     5401 actatttttt gtaccaaaat ttccacttac ttgtgttgtt tctaatttaa tttgctttcc
     5461 agcatctaat ttttttaatt gatcataatt tataggaatc agtctagagc taaattgatc
     5521 cattgtgtta agagctagag gtgaaagccc ttttttggga taagtatctc caggagatag
     5581 attattgcca atttgatttt cttgtgcttt gatagttgat aatgtatctc catctaacac
     5641 taaatttgtt gttggtgtca ctttgtacat aggtgcagta cctgtgttgt aatatctaac
     5701 atttgcattt atatatgcag attctccttt gtttatactt aatccagtat tccatgattc
     5761 tccattacta tcttgaacag cagttgaatt atctgttgta tgggaataat ttgtagttac
     5821 attagctgtg aatccatttt gatatcctac attaacagac acaccagctg tattagattc
     5881 agttttactg ttagtagtag ctctggaaac agttttacct tgatcagtag aggcatgttc
     5941 atttgtagat ataattaatt tttccatacc tactccaaca attggatatg ctgcaactaa
     6001 cggatctctt gcttcagtct ttatagcctt gtcaaaagaa cctgaagctt tttcataatc
     6061 tgtatatgga tctccagcag tatttgactc taaataattt gatacatatt tcttatagcc
     6121 ttgttctgca aaactatctt cccacttaac tgcaattaag tccttaatag tatatccatt
     6181 tcgttcatat gaatctggta tattatcatt atctgtatcc aaatcttcgt cttcccaatc
     6241 agacatcaac tttgggtcaa agaaattgtt atttgggata aatggatcat ctttttctat
     6301 attagaataa tctcttaaga ataaattttc ttctggtata attttcttca taccatctaa
     6361 ttcccaataa agattaggtg atgataaatt atctattgaa cctaaatttt tatcttgtag
     6421 ctctattcta actttatatt ctttaccctt tttcatatta actttaagtg tatttgatat
     6481 agtactctca gtatttactt gcattaagac atcatctcta tcagttgata atgtatattc
     6541 accatcctta gaaggaatta ttcttcctgt ccatcgtata gattttacat ctgatttgtc
     6601 tttatccaga agtttatcta ctttcttttc ttcaaatttt aaattaccat cttttatggg
     6661 tgccattaat tttaaatctt taaagtgctc atctgtgaaa taatatccca ttaatccatt
     6721 gtttgggagt atatcttcat ttacaatttc ttttttctta ttagaatgat tacttgtact
     6781 agtttgagca tatacaggat atactagtgc ttgactaact atagctgtaa gtgttaaaaa
     6841 acttaatacc tttttattcc tcatttgtat tttcattgtt tctcctcctt aaatttgaaa
     6901 aatattaaat tatttatgtg aaaaaatatt aaggtatcaa tgttgcatca acaattaatt
     6961 ttgttatagt accatctttg taagaatcaa ttttattgat tttaaatttg cttccatgat
     7021 ttaaaagcac ttcatattca cctgcataac ctggaatagc tgatagataa gctccaggag
     7081 aacctttagg tatagttata cgtagtacta tttttctttt agcaaatgca ctcatattca
     7141 cactaccaat actagtacta ataaagtttg gataagacag tgcttgtcct tcccattttg
     7201 atttaaaagc atctatattt tctagtttgt taaaatcata ttcaggggaa gtaagagtta
     7261 aaccaaattc ttgaggacca gatcttctat atacagttaa attagttgga ataggttcac
     7321 gttttaatgc attttcaatg tttgtgattt tagaatctaa ttctgggtta ggattattta
     7381 ctggaccatt tgatattaaa taattattaa ttgcagtata tcctccacgc atataatcat
     7441 ttacatcagc aagttcatta ggtgttaatt tattactcca atcattataa tttgctttac
     7501 cccaagaatc ccccttactt acatcatctt taaaatcaag actacttaca acagatgctt
     7561 ctgctttaat atagtgcttc ccatctataa ctatacgaac aattttatct atttttatac
     7621 tatatccttg ctctattaat gtacttacat tgttagtatt tgtatatggt aacataccag
     7681 tatttctagg taattttaag tgcataagta atggtgtagg tttttcatca ccttttccag
     7741 gttcatataa agaaatatct ttaaatccat cttgcttaaa taatttgttt tgtatagttt
     7801 ctttaaactc attaaatttt tctaatgaaa tttcgttttg attttctgtt cttattactt
     7861 tattaaatgc aaatttttct ggagattcaa aataatagac atacataggt ttatctattt
     7921 tattttttga tatagcattt cgaagttctt tatattcttt ttctcgagaa tttgcttcta
     7981 tttgataatc ataaaaataa tttcttgtct gagaatattt acttatttct acagaatctt
     8041 ttttatatga ttctaatgct tctttttcag atctttcaag tttttgctct attctttctg
     8101 cttcttttct ttcccattct ttagcctttt ctttatcttt aagaaaatct tctggtcttt
     8161 ctataggagc cttgtaagta gtattgcaaa ctttttcact atagctttgt aagtcttgtg
     8221 cataaatgtt attaggtaac aaaccaccta aagttagata taatatcaac aatatagata
     8281 tacaattact aatcctttta tgtttcctaa attttttcat ttattctccc tcccaatatt
     8341 agtttaatta actcttgaac tttaatttat ttagattata taatctaaat aagaattttt
     8401 tatataaatt tcataaaata tgtaactata tatcataaaa atatgtattt atatgatatg
     8461 tcagtacaaa ataacaactg aatattttaa agagactatt tcatactttt atgatttaat
     8521 gagctatctt gttgaatcat aatctaggaa caatggttgc aatgtctaat agaaattgta
     8581 tatgatatag tcattatgtg ttttttcaca aaacgattat tatatagagg attcttagga
     8641 actacttttt ttgtataaaa aaagagatgg ttcaaattct tatcttgaga catctctttt
     8701 ttctatttat tatgttttaa taatgttctt taaaatattt ctatatgtat tagatatacg
     8761 acatatatgg ccattactca ttttaatatt atttttcttt aggtcgagag aaacaatatg
     8821 atatttatta actacaaaag atttatgtac acgaataaag catgcatcta aatctgataa
     8881 agaccttaaa cttttataga attcaaatat tcttgaagta gtcactaatt ttattttatg
     8941 tggaatagcg cttgtttcaa aatatataac ttcatcaaat gaaattttcc atagtgagga
     9001 atctgagtag atagtaaaaa aattagagcg ataatttttt tctttaacgt atcttttatg
     9061 agcaatttta atacaagctt ttattttact ttcagcaatt tccatatcat gttttaatat
     9121 aaagtccata acttctaacc tataatcgaa gatccctaaa atcatttcta tatgatttgt
     9181 aagaaatatg attggactaa tataatctga ttctctaatt ttttgtgcta tccataatcc
     9241 atcacataat tcattactta tcttgtattt tgcatctaca actataaaat atatttttac
     9301 tttatttttt tcagtaagtt cttttaataa caaatttgca ttataatata atcttataac
     9361 atcgttatca aaaccttctt ttattaaaat attatttatt ttttctttta gttttattcc
     9421 aaaacaaaca tcattatcaa aaattaatat atccactaaa taccctccta taaaaaattc
     9481 aaaagtaaaa aaactaaaag tattttataa aaaatacgta aaataacact aggagataaa
     9541 taaaattaag gatagttaga attaaggtat atttatg
//
LOCUS       DACTGW010000150         9544 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.150, whole genome shotgun sequence.
ACCESSION   DACTGW010000150 DACTGW010000000
VERSION     DACTGW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.150"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            229..942
                     /gene="rgbR"
                     /locus_tag="I9573_002662"
     CDS             229..942
                     /gene="rgbR"
                     /locus_tag="I9573_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HAT4797272.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDDEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNAAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            936..2315
                     /locus_tag="I9573_002663"
     CDS             936..2315
                     /locus_tag="I9573_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAT4797273.1"
                     /translation="MLNFMNELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGSYFSNINYLFYILAFIAWGGYYNYFYKMDYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLVFSFFRKS
                     IKLISQDKRNYVYLLFALLANTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITKSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     EQDTDSWGD"
     gene            2556..3245
                     /locus_tag="I9573_002664"
     CDS             2556..3245
                     /locus_tag="I9573_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797274.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMAGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEEFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            3464..5836
                     /locus_tag="I9573_002665"
     CDS             3464..5836
                     /locus_tag="I9573_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HAT4797275.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAEGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            5851..6753
                     /locus_tag="I9573_002666"
     CDS             5851..6753
                     /locus_tag="I9573_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAT4797276.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EKMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            6773..7324
                     /locus_tag="I9573_002667"
     CDS             6773..7324
                     /locus_tag="I9573_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4797277.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSYVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            7488..8360
                     /locus_tag="I9573_002668"
     CDS             7488..8360
                     /locus_tag="I9573_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAT4797278.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            8524..9504
                     /locus_tag="I9573_002669"
     CDS             8524..9504
                     /locus_tag="I9573_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797279.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRVYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
BASE COUNT         3911 a          874 c         1661 g         3098 t
ORIGIN      
        1 tattttttta atattttaag acatcttctt ttgttagtaa agtactgttt ttaattatag
       61 taatttctgt ctttaaattg aaatatcaaa taatagttgt attggattga gtattgataa
      121 aaataattta aaaatgttat cctaaagtat cagatataca aaattatctt taaatatatt
      181 ataaatattt taattgtatt aactagaaat tatgtaagga ggcattttat gattaagata
      241 gcagtttgtg aagatgaaaa agaaacacaa cttttaatag aagattacct tgagaatata
      301 ttaaaagata taagtataga atatgagata caaaagtata tatctggaga agagttactg
      361 gaaagtaatt taaaagatat agatatatta cttcttgata taaaaatgga aaaattaaat
      421 ggaatggata ctgcaagaaa gattagagaa gtagacgatg aaatggaaat aatatttgta
      481 acttcattaa tagattatgt gcaagaaggt tatgaggtaa gagcttatag gtatttacta
      541 aagccaatag agttggaaga actaaaaaaa catgtgctaa cttgtatcaa agatattgaa
      601 ataaataagg aaagtcatat tacaataaaa aataagtcta atacatataa gatttattta
      661 aatgaaataa aatatataga agttcaaaaa aaagatatgc taatacacac aataaacaag
      721 aattttgata taaaatatag tttaggtaaa atagaaaaag aattaaatcc atataaattt
      781 ataagatgcc ataaaagttt tatagtaaat ttaagatatg ttgaaaatat aaagcctaat
      841 gctgcaatac tggaaagtgg agaagaggta cctattagta gatatagata taaagaagtt
      901 aaggagaaat ttttaaaatt tcttggtgat acaatatgct aaattttatg aatgagctat
      961 tagtagtttt atctatattt atacagtttt ctataatgaa cggaatttta atttataaaa
     1021 aggatagtaa aaaaaatgaa aaaatagtat tctatattgt cttgtttctt atttatatta
     1081 taattcagat aagtagatta tttaatggag gaagttactt tagtaatata aactatttat
     1141 tttatatatt agcatttatt gcttggggag gatactataa ttatttttat aagatggatt
     1201 atattaagta ttccattttc ttttatctat ttagtgtata ttataaatgt ttggatgctg
     1261 ttatatatag aatactattt tttgcaatta caggtaatgt tatttcatct aaagaatcct
     1321 tattagtacc tcacttagaa tctataaaaa ttcagatata tagtaattta ataattattc
     1381 ttatatatct tgtatttagc ttttttagga agtctataaa attaattagc caagataaga
     1441 gaaattatgt atatttatta tttgccttat tagctaatac aatgaatatg cttgtaaatt
     1501 atgtattaaa aaaattagaa cggtttgggt cattacattc agaaggatac tattttgata
     1561 attttgttaa tccaaagttg gtaggagcta gtagtatatt tcttatatta ttgtttaaag
     1621 aaataataaa agagaataga ttaaaatctc aagctgaatt aataaaaaat aagttagata
     1681 tgcaatatgc gcattattta agtattcaag aatctcatat gcgagttaag aaactatatc
     1741 acgatataaa taaccacatt tattgtatag acaaccttag aaacaatagc aaagaaatta
     1801 atgaatatgt taacaattta aaagatgaaa taaaaacatt taaatatatc tataatacag
     1861 gaaatatgat attagacatt ataatcaacg aaaagagtga aatatgttta aaaaaaggaa
     1921 ttaaatttac ttgctctata aatttttcaa aagtaaattt tgtaaaacct atagatgtgt
     1981 caagcatatt ttctaatatt ttggataatg caatagaagc atgtgataaa atagtggatg
     2041 aaaatattaa taaatatatt agaattaaag gaactataac aaagtcattt tttgtactta
     2101 aatgtgaaaa tagtaaacta aatcagctta cttttaataa aaatatattg ttaacaaaca
     2161 aaatggataa gttcgtacat ggaataggta ttcagagtat taaatcttct ttacaaaaat
     2221 ataatggaga attattattt gaaaatagta tcgataaatt tatactaaat atatatatac
     2281 cattggagca agatactgac agttggggcg attaagttac cagttgggac atatgtattg
     2341 atacaaaaaa ataaggatgt tatcttttaa taacaaaaga taacatcctt atttctgtat
     2401 taaggagctt ataattttac taatagtaag ttataagaat tggataggaa ggtgatatgt
     2461 attgaataat aaatctttaa tataaagaaa atataaatat attctaatgc aatgaataat
     2521 actttagagc ggttagtgtg atgggaggat tgtgtatgaa agtaaaaagg aatataaata
     2581 gagtggcaat aattgtggct ttaaatctta gcttagcatt gatggcagga tgtatgtctc
     2641 ctgtatctga taagaactta agtagctcag aacaaattag taaagattca ttgaaagaac
     2701 aagttgaaga taaaaaagat gaatctatcg agctatttaa taattactca caaaagatta
     2761 atgatgcgaa aggtagagaa gaagtaaaaa aagatttatc tcaaagattt tcgaaagcta
     2821 taatagacag tggttatagt attactgact caaaatcaga tggtagcttt gtagtatcta
     2881 taaatggtgc tgaaaaacca cctgaaaaaa atattcgaag tttattctat tctatgtctg
     2941 aagacgataa agaaggaaaa gtaacaatag atattctctg tgctaaagag tatccagaag
     3001 atgataagtt atctgagagt gataaatata ttaaatttat atacaattta tttaaatcat
     3061 tgactaatac gaacttaact gagaaagaaa ttttttctat ggttgaagaa gaatttaata
     3121 agggagtagg tgttgtggag cttccttata tgaaggatat acatgttgaa gttaacaaag
     3181 ttaaacaatc aactaaggtt ttaaaactaa gtctagtata caaatttgat atttcaaatc
     3241 attagtatat tagaaataac tataacaata tagaaaatat atcatagaaa atttttttag
     3301 attttataaa tttaacaaaa aaagtattaa tatataggtg ttcaaggtag atgaaaaaaa
     3361 aagttatttt gcttccattt tatgctaaag gacagtataa tataattaaa gtatgttata
     3421 acatacaaaa atgtagtata tattatccaa agggggattt taaatgagtc aaactaatag
     3481 aatagaagca tttagacaag aatatattaa ttctaaacct atgatatgtt gtgaaagggc
     3541 tagaattttt acagaatcac ataaaaaaac tgagggagaa gctatctgta taagaagagc
     3601 taaagctttt ttggagacat gtaaagaact tcctataaaa atctttgaaa atgaattaat
     3661 agtaggtaca gcaggaaaat ttagaagaac agggatattg acaccagagt tttcttggca
     3721 atgggtagac aaagaaatgg atacttttga taaaagaact caagacccat atgtaatatc
     3781 aaaagaacaa atagaattta taagaaaaga aatatttcca tattggaagg gaaaatcttt
     3841 ggaagaagtt ttcttagcaa gaatcccaga agatacagct aagatattag tagatacagg
     3901 aataatagat aatgattcta agtggagaca ggcagttggt gaagtaacac cagattatca
     3961 agatatattg tttgtaaaag gatataaagg gattaaggaa gatgcagata aaaaaattaa
     4021 agaattagat atatcagttt cagaaaatat tgaaaagata gatttttaca aatctgtttc
     4081 tatagttgca gagggtatta tgacattggc acaaagatat tcaaatcttg caaaagagat
     4141 gagtaagcaa gaaacagatg aaaagagaaa gttagaactt ataaaaattt cagagatatg
     4201 tatgaatgta ccagcaaacc ctccaactaa tttttatgaa gcaatacaat ttgtatggtt
     4261 tgttcaatta ggtggtattc tatcagaaaa tccattggca ttaaatttag gtagatttga
     4321 ccaatatatg tatccatatt atgaaaatga tgctagagaa ggtaaaataa ctgaatcaga
     4381 agctcaagaa ttgatagaag cactttggat taagttatca gaatgggtat ggactatatc
     4441 agcaaataca gcaaattact ttgcaggata taatcaattc caaaatctta ctgttggtgg
     4501 taaaaagaga aatggtactg atggaacgaa tgatatctca tatatgtgtt taaaggctac
     4561 agaaagtgta aaaactcacc agccaggatt aagtgttaga gtgagtcaag gagcaccaga
     4621 taatttcgta atggcagttg ctaaattggt aaaacaagga actggtttcc ctgctataca
     4681 cagcgatagt gcaggggcac aaatgttatt acaagatgga tatgatgcag aagatgctag
     4741 agattggagt aactgtggtt gtgtagttcc tcattttaga aaaacaggac agtggacttc
     4801 agccgtaaat atcaattttg cagcagcttt agaatatgct atgaatgaag gtaagagtag
     4861 attaactgga gaaaaaatgg ggttagatac aaaaaatatc acagaattta cttcttttga
     4921 agaacttaag gatgagtttt taaagcaatt agcatatctt gtaaaaagtt ctgttatagg
     4981 aactactgtt gctcaacaaa ttcataaaga aatggtacca agaccatttt tatctacttg
     5041 tgtagatgga tgtttggata agggagttga tttaagtaaa ggaggagcaa aatataatat
     5101 aggtcctgta ttaactggta tagggttagg tgtagtttca aattcattgg cagctataaa
     5161 aaaattagta tttgaggata aggtgactac gctagaagaa ttaacaaaag cactaaataa
     5221 tgattgggaa ggttatgaag aattaagaaa acttgcgttg gatgttccta agtatggaaa
     5281 tgataatgat tatgtagatt cattagcaat tgaagtttct gatttttatt atactgaaac
     5341 tagaaaatac aaggatattt ttggctctaa atttaatagt gcatttatgg gtatatcaaa
     5401 ctatgtacca actggaaaaa tcgtaggtgc aactccttgt ggaagaaaag cgacaaaacc
     5461 tttgacagag ggagtttctc cgtttgttgg tactgataca acaagtccac tagcagccat
     5521 gaaatctgca tcaaaaataa atcatgatgt tcatactggt ggaacacttc taaatttaag
     5581 actgaatcaa gatttggtgg aaactgaaag aggacttaga aatctaacat ctatgattaa
     5641 atcatatttt gctttaggag gattccatgt acaatttaat acgatatcaa atgatactct
     5701 attaaaagct caagaaaatc cagaagaata taaagattta ttagtgagag ttgctggata
     5761 tagtactcaa tttgttaatt tatcaagaga aatgcaagat gctataatag ctagaaattc
     5821 acatagcaat ttttaattat gaggtagatt atgagtaaaa aaggtagagt tgtaaaagta
     5881 caacattttt cagtaaatga tggagatgga ataaggacta ctatattttt agaaggatgt
     5941 aaattaaaat gtaagtggtg ttctaatcca gattcctgga gtaacatagt aaaattaggt
     6001 gttatgaaag ataaatgtgt atcctgtaat agatgtatag atgtttgtcc tcaaaatata
     6061 agttcactat ttgatagaac gcaaataaat aataaatgtg atttatgtgg agagtgtatc
     6121 aaggtatgtc ttaaagatgc catttgtata atgaccgaaa aaatgagtgt tgaagaaata
     6181 gtagaggaag ttgaaaagga ttttatattt ttctttgaat ctaatggagg gataactttt
     6241 tctggaggtg aacctacact tcagattgat tttttaagag aattagttga tattttttat
     6301 gataaaggca taaatattgc aattgaaact tgtggatatt ttgattggaa taaggtaaat
     6361 gatgtatttg agaaaataga ccacatattt gtagatataa aatctatgga tgataatatt
     6421 cataaagaat atacaggagt aagtaataaa attatattag ataatatatg taggctttca
     6481 aagttgaata aatctatggt tataagagtg ccaattattt atggagtaaa tgatagtgaa
     6541 gaaaatataa gaaatactgc attatttgtc aaacaaaatg ttccaggagg caagatggag
     6601 ttacttccat atcataaatt tggaatcgat aaatataagg cattagggct tgaagattat
     6661 atatatgaat ttgatgaaat ttgtaacaat catatgttaa aattaaaaga gatagtagaa
     6721 cttacaggag taaaaatcat agaatacaaa tagctatcag gaggatttta acatggataa
     6781 taaatttcct aatgtgggag aaaatttaag gttattgaga caagaaatgg gcataagttt
     6841 agacaaagct tcaaagatga caggtgtcag taaagcaatg cttggacaga ttgagagagg
     6901 tgaatcaagt ccgacagtat ctactttatg gaaaatttca tctggattta aaataaattt
     6961 tactacacta ttaaatgaaa atacaaatac ctatgaggtt ataaagaaag aagaagtaga
     7021 gcctattgtt gagcaaaaag ggaacatgaa gttatatcca atataccctt ttagccctca
     7081 aagaaggttt gaacttttta taattgagct tgaggaaaac tgtactcatg tttcatctac
     7141 acatagttat gttttagaag agtatgtact cgtaatagaa ggtaaacttg accttaatgt
     7201 ggatgggaaa acttatattt tagagtctgg acattctatt aggtttgatg gaactttaga
     7261 gcatatatat aaaaatttaa ataaaggaaa aactatattt cacaatatag aggtttatag
     7321 ataattttat agttatcctt ttctaaaata aatgaaaatt ttagaaaagg ataacttatt
     7381 tttataatgt cgaaaagatt tatatgtatg tgatgatgtt attgttttaa aaaaaacata
     7441 aaagcattat aataatatta acaaaataga ataaagagga agagaatatg attataaagg
     7501 tttcaatgac agtattagta ttaatattat tgtatattct gtataagtat gaaactcagc
     7561 atatagaaac gacagaatat actattttca acaaaaaaat tccaaaggaa tttgataact
     7621 ttaaaatagt tcaagtgagt gatttacata ataaggtgtt tgataaaaat aataaggtat
     7681 tgatagataa aattgaatca ttaaacccag atgttatatt tataacagga gatttagtag
     7741 atggagaaaa taaaaatttt caagttgcat tagatttaat agataatttg gttgaaaaat
     7801 atgaagtata tcatataatt ggaaatcatg aacaaaaatc tttaataaaa aagtataaac
     7861 atctatataa ggattatttt aaggaactat attcaaagaa aataataaat atggaaaatg
     7921 aatctgtaag aatatataga ggtaatagcc atcttaattt atatggttta attataccat
     7981 tggaatgcta tccatattta tttgctaata acaaaaaatt agaactaagt gacaattttg
     8041 tagaagacaa tctaggtaaa gttaatgaaa atgaatataa cattctttta gcacatacac
     8101 catttttctt tgaaaaatat gcaaaatggg gagcagattt ggttttagca ggacatgttc
     8161 atggaggtat aataagaata ccttttgttg gaggtgtatt atctccaaat agagagttct
     8221 ttcctaagta tgactgggga aaatatgaaa aagataatag cactatgatt ttaaataaag
     8281 gcttaggagg ctcaaaagtt ttaattaggc ttaattgtaa accagagata gttaagatta
     8341 cattgaagta tgagaaataa gattttatat aacatatata aacatcaata acatataaat
     8401 aaggttgatg ttaaatccat ttaaacatat ataatgacca attttgaata attgttgaat
     8461 taataaaaat aaagtgctat ttttagcata gaatatttaa actatggggg ggagtaaaga
     8521 atcatgggtg atacaagtag attttatgag tctgtaaaaa ataaatatcc gaaattaaaa
     8581 gatggaacta gggttcatct gtggccaaaa gaaaacatta taattggtcc tatttatgat
     8641 gaagaagatg gatataaaaa aagtatcgga aatgtggata gcttaagttg gaagcaaatt
     8701 gttatactgg ctgaatgtga tgggaaaaat actactgacg atataataaa tatattatcc
     8761 aatagatata gaaatatcca taatatgaaa gaaaaagtta tggaattttt tatgttttat
     8821 gagaatgtat acttaacgtt tgaagatgat atacagacac tatcaagtgt atttgagata
     8881 acaggaaata aatcatacat tactcctttg tactttacta tagaaataga tggagataat
     8941 aatacaaatg attatttttc tgaaatcaca agtttattga actgcatgta tgagaaaggc
     9001 tgtagattta tagaggttat tggtgaggat attttaaaaa ataaaagtat gagagaaata
     9061 tttcaatata tgttagacca ttttgattta attgtggtaa ctaaagatag ctttactatt
     9121 gatagaagct taataaaaga attagataat tatcgtcata aagtgatatg gaaagtatat
     9181 tcaaaaaatg atgatacacg tattaaatta aaagaagata taaaaataac cagtttaata
     9241 aaaaggggtc acactgtagt aagaggagac agagaaaaag caataaaaat agaagatatg
     9301 aggtttaaag atacaaagga gaggttttca aaatatggag cagagtggag tcatttatat
     9361 atttcttctg atggtagtgt aaaatcttat tcatttcaaa aagataaaag agtctattta
     9421 ggcaatatat ttaacaattc tgttgaagaa attttcttag agatgcaaaa taaaattgac
     9481 aaactagaaa ttgtgtatca ataagggtaa agtttaagat ttatataaga tttatagatt
     9541 gttt
//
LOCUS       DACTGW010000151         9538 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.151, whole genome shotgun sequence.
ACCESSION   DACTGW010000151 DACTGW010000000
VERSION     DACTGW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.151"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            204..662
                     /locus_tag="I9573_002670"
     CDS             204..662
                     /locus_tag="I9573_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT4797280.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFILGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLRTLKSL"
     gene            723..998
                     /locus_tag="I9573_002671"
     CDS             723..998
                     /locus_tag="I9573_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4797281.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1057..2331
                     /locus_tag="I9573_002672"
     CDS             1057..2331
                     /locus_tag="I9573_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843259.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAT4797282.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2461..3513
                     /locus_tag="I9573_002673"
     CDS             2461..3513
                     /locus_tag="I9573_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAT4797283.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     AVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDIDPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3513..4544
                     /locus_tag="I9573_002674"
     CDS             3513..4544
                     /locus_tag="I9573_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAT4797284.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKDIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4636..5448
                     /locus_tag="I9573_002675"
     CDS             4636..5448
                     /locus_tag="I9573_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAT4797285.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5451..6371
                     /locus_tag="I9573_002676"
     CDS             5451..6371
                     /locus_tag="I9573_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HAT4797286.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6546..6992
                     /gene="rpiB"
                     /locus_tag="I9573_002677"
     CDS             6546..6992
                     /gene="rpiB"
                     /locus_tag="I9573_002677"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HAT4797287.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7031..7696
                     /locus_tag="I9573_002678"
     CDS             7031..7696
                     /locus_tag="I9573_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAT4797288.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7806..9308
                     /locus_tag="I9573_002679"
     CDS             7806..9308
                     /locus_tag="I9573_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAT4797289.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTNIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNNSNLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
BASE COUNT         3860 a         1054 c         1853 g         2771 t
ORIGIN      
        1 aagtgattaa caagtaaaaa ttaaaataaa aatcaaaaaa aatttatcaa tgaaagtcag
       61 ctaaaatcct ggaagagagc attattttcg ggaaaacatt ttcttttgtt tcacaaaaaa
      121 agccactttt tggcacgcaa catgctttat ataaagtcaa gaaaaactaa ggtataagac
      181 aaaggcaatt aggaggtgta aatatggagt ttataaattt tgtaagtgat aaagttatag
      241 accttaaaat ggattgtaaa acgaaagaag atttctttaa agaaatacat aataaagttt
      301 ttgatttagg ctttgtaaaa gaagaatttg gagaaaaaat attagcaaga gaaaatgtat
      361 ttccaacagg tttaaattta ggtgattatg gagtagcaat accacataca gatgcagaat
      421 atattaaaga gcaatttata tcagtctgta cttttaatga accagtagta tttagttcta
      481 tggaagacca agatgaaaaa gtacctgtaa atttggcttt catattagga cttaatcaac
      541 cacacagtca attatctgta ttaacagaat taatgggaat tatgcaaaat aaagagttag
      601 ttgataagtt gatgagttct actgataaag aagaagtttt aaggacacta aagtctttat
      661 aaaaataaaa aagtactaaa aataaaaaat ataaaaataa aaattattag gaggaaataa
      721 aaatgaaaaa aatattagta gcatgtggag caggaatagc aacatcaaca gtagtttgtg
      781 acaaggtaga gagattagtt aaggaaaata atatacaagc agaagttata caatgcaaaa
      841 tagctgaatg tgcaacaaag caagaaggag cagatttaat agtatcaaca actatactac
      901 caacaactta taatatacca gcaataaaag caacagcata tataactgga gtaaatacag
      961 cagcattaga taaaaaaata ttagatgctt taaaataatc ctatcataga tacattgaaa
     1021 taatatttta taaaacagaa aaaggaggat ttataaatgg ataaattatt agcagtagta
     1081 caatacattt taaatatggg accgacagta atactaccaa taacaattgt agtactagga
     1141 atgatattta gaattaagtt tacaaaagct ttaaagtcag gtcttacaat tggtatagga
     1201 tttgtgggta taaacttagt tgtaaaccta ttaacatcaa cactagggcc agcggctcaa
     1261 ggaatggttg aaagattcgg acttaattta acagtaatag atgttggttg gccaacaaca
     1321 gcatcagcaa catgggcttc accagttgca ccaatattaa taccagtttg tttattagta
     1381 aacttactat tattatactt caataaaaca aagatattaa acgttgatat atggaattat
     1441 tggcacttta tagtagcagg tgcagttgga tatatagtaa caggaagtta tgtttgggct
     1501 atattctgtg caatactaat ggagttatta gtactgttta ttgcagaaaa gacagcaaaa
     1561 aatgttcagg agttttatga tttagaaggt atagcattac caactggttc aacagcttca
     1621 tttgctccac ttggatattt agtaggtaaa tttgttgaaa aaataccagg aataaataaa
     1681 atacaagcag acccagactc tatacaaaaa agatttggta tatttggaga accaatgatg
     1741 atggggatta tactaggtat aatacttgga gcacttgcgg gatatgatgt agcaggaata
     1801 ttcaatgtag gtatatcaat gggtgcagta atgttactta tgcctagaat ggttagaata
     1861 ttaatggaag gtttaattcc aatttcagaa tcaataagag atttcttaca agcaaaatat
     1921 ggagacagag aactttatat aggtctggat gcagcagtag caacgggaca tccagcagtt
     1981 atatcaacag ctcttatact tgttcctata acgttgttct tagcagttat actaccagga
     2041 aacaaagttt taccatttgg agacttagcg acaataccat tttatatggc atttatagtg
     2101 tgctttagaa aaggtaatat agttcaatca gttataacag gaacaatagt aatagctata
     2161 tcactttata tggctacaaa ctttgcagat gttcatacac aattattaca acaagcaaat
     2221 atgatgccag aaggaacaac aatggttaca agtatagata caggtggaaa cttgctaaaa
     2281 tggggaatat tgaaattatc tcaattagtt tcttcatttg gaatattcta atatgtagtt
     2341 aatagagatt actctttttt agtaaagtat gcgaaaattt aacatttagc agctactaaa
     2401 aaagagtaat attttttata aatttttaaa aatacaaaat acaagaggag gagatttatc
     2461 gtgaaagcag cagtattaca tggcacaaat gatatgagat ttgaagatat agaaataaaa
     2521 ccatgtgaaa gtgatgaagt aaaaataaaa gttatggcag caggaatatg tgggtcagat
     2581 cctccaagag tattaaaaca ttggaaatat ccagttccag ctataccagg acatgagttt
     2641 tctggagtaa ttgctgaagt tggtaaggat gtaaaaaatg taaaagttgg agatagagta
     2701 gttgcaattc cttttatacc ttgtaatgaa tgtgaatatt gtaaaagagg attattttca
     2761 ttgtgtgatg accatggaat gttaggagct aaaagttttg gagcttttgc agagtatgta
     2821 aatataaaag ctacaaatgt acttccaata ggagatatgg actttgaaga tgctgcaatg
     2881 atagagccac tagcagttgc tatgcatgga gtacttaata taggtgttca agttggagac
     2941 gctgtagccg taatgggtag tggaacaatg gggcagttgg taatacaagg acttaaaata
     3001 gcaggagcag gaacaattat agcagttgat atatctgata ataaattaag agaatcaaag
     3061 gaattagggg ctgatataat aatcaatgct aaagatattg acccagtaga aaaaataaaa
     3121 gaacttactg gtggaaaagg cgttgacata gctcttgaat gtgcaggttc aaaaataact
     3181 caagaacaat gtttacttat aactaaaaag aaaagtaaga taggattttt agggatagca
     3241 tattcagata taactttatc agaagaagca tttgaaaata tatttagaaa agaactagaa
     3301 ttaaaaggtt tttggaactc ttattctgca ccatttccag gacaagaatg gactaaggga
     3361 atcaatctag ttaatgaagg aaagataaaa ttaaaagaaa tggtatctca tagattctca
     3421 cttgaagata cttacaaagc atttgaaatg ataagagata gaaaagaaga atttaacaaa
     3481 atattaattt taccacaagg ggttgaaaaa taatgaaagc agtagttaaa actaaaccag
     3541 gctatgataa tgtagaagtt ttagaagtag aagaaccaaa agctacagga gacaaggtta
     3601 aaataaaagt agaatatagt ggtatatgtg gttcagatat acattcattt aaaggtgaat
     3661 atgcaaatat aaaagcccca gtaacattag gtcatgaatt ttctggaatt gttgtagaag
     3721 taggagaaga tgttaaagat ataaaagtag gagatagagt tacatcagaa actacttttg
     3781 caacttgtga gaaatgtata tattgtgaaa ctaaagatta taacttatgc ccaactagaa
     3841 aaggtattgg tactcaagta aatggaagtt ttgctgagta tgtactatca agagaagaaa
     3901 gcatacatgt acttcctgaa aatgtatctt tattatcagc agcccttaca gagccactag
     3961 cttgttgtgt tcatgcagct cttgaaaaga ctactataga aagttctgat acagttttaa
     4021 taataggacc agggccaatt ggtttattat tagctcaagt tgttaaatct caaggtgcta
     4081 cagttataat gtctggtgtt acaaaagata aggaaagatt agaaatagct aaaaatcttg
     4141 gagtagatag aactgtaaat gtaatggaag aaagtttggc agatgtagtg aatgaaatga
     4201 ctgatggaaa tggagtaaac aaaggatttg attgttctgg gtttatgcca gcagttaacg
     4261 aagccttaag actgcttaga aaaaaaggta ctttcgtaca agttggaatc tttgctaaaa
     4321 aattaaatga aatggaccaa gaagcaataa tccaaagaga gttacaatat ataggaagta
     4381 gatctcaaaa gccaagttct tggattacag cattagattt aatggcaagt gggaaagtaa
     4441 atacagaagc tttagttact agaactgttg gtttagacga atttaaagaa ggtattacag
     4501 ctttaatgaa cggggaagaa ataaaagtag cagtaaaatc ttaaaaaaac atttgtatta
     4561 ttttaaaaaa aattgtaaaa tctactgtag gataaaaaaa tgtaaggtct taaaaaataa
     4621 caggaggaag aaagtatgag cactcttaga gatcattcta ataatattag aaaaaacata
     4681 ataaaaatgg ttgcagaagc taaaagcgga catccaggtg gttcattatc aatagctgat
     4741 atgttgacag tattatattt tgataagatg aacgtatgtg cagaaaatcc aaaaatggct
     4801 gatagagata gatttgttct atcaaaaggt catgcagcac cagctttata tgctacatta
     4861 gcagaaaaag gatttttccc agaagaagaa ttaataacac taagaaaatt tggttctaag
     4921 ttacaaggtc acccagacat gaaaaaagta gcaggaatcg atatgtctac tggttctctt
     4981 ggtcaagggc tatctgcagc aaatggtatg gcacttgcag gaaaattaga taacaaagac
     5041 tatacagtat atactatatt aggtgatgga gaaatccaag aaggtcaaat atgggaagct
     5101 gcaatgacag catcacatta caaattagat aatttaattg catttgttga cttaaatgga
     5161 ttacaaatag atggttctaa tgaagaagta atgaatgtaa atccagtaga tgacaaattc
     5221 aaatcttttg gatggaatgt aatagtaata aatggacatt cttttgaaga aataggaaat
     5281 gcagttgatg aagctaaaaa agttacagga aaaccaactg taataatagc taaaacagta
     5341 aaaggtaaag gtgtttcatt tatggaaaat aatgctgcat ggcatggaac agctccaaat
     5401 gcagaacaag cagagcaagc attgaaagat ttagaaggag gtctatagga atgggaatag
     5461 caactagaga agcttacgga caagtgttaa aagaacttgc agaaaataaa gatatagtag
     5521 tattagatgc agatttagga aaagctacaa aaagtatatc atttaaagaa gttgccccag
     5581 atagattttt tgacatgggg atagcagaag gtgacatgat tggtacagca gcaggtcttg
     5641 ctacttgtgg aaaaatacct tttgcgagta cttttgcaat atttgcagca ggaagaggct
     5701 atgagcaaat aagaaactct gtagcatatc caaatttaaa tgtaaaaata gcagctactc
     5761 atgcaggtgt aacagtaggt gaagatggtg gtagccatca agctatagag gatatatctc
     5821 taatgagagg tataccaaat atggttgtat taaatccagc agatgcttta gaagcaagac
     5881 aagctattct tgcatctatt gattataatg gacctgttta tataagatta ggtagagctg
     5941 caactcctga tgtaaacagt gaaaattata aatttgaaat aggaaaagga actgttttaa
     6001 gagaaggttc tgacataaca gtaatagcta caggaataat ggtagcaaaa gccttagaag
     6061 cagcagaaga acttgctaaa gaaggtataa atgtagaagt tgttaatata tctacaataa
     6121 aaccattaga tgaagcatta ataaaagaaa ctgctaagaa aactggaaaa gtagttacag
     6181 cagaggagca cagtataata ggtggtttag gttcagctgt atgtgaagct ttagcagaaa
     6241 ctaaagatgt agtagttaga agaattggtg ttaaagatgt atttggtcaa tcaggtacac
     6301 cagctgattt attaaaacac tatggtttaa ctacagaaga tatagtaaaa aatataaaag
     6361 agttgttata gatataaata tatctaaata acaaattgat ttgacataag tcaattaaag
     6421 ctgtcttaaa aatagaaaga aatttctaca atgggacagc tttattttta caatacttta
     6481 tttttatgat aattataaaa tagtctgaaa gtaatcaaaa aaattataaa aagaggtgct
     6541 atttaatgaa aatatctatt ggatgtgacc atggtggtta tgaattaaag gaatatataa
     6601 aggaaaattt aataaataaa ggtatagaag ttgaagatgt gggtacaaat tcaaaagaaa
     6661 gtgtagattt tccaatatat gcaaaaaagg taagtcaaaa tgttcaaagt aaaaaatctg
     6721 atttaggcat actttgttgt ggaacaggaa taggcatgtc tattgcagca aataagttta
     6781 aaggaatcag agcagctgtt gtttctgact gtttttcagc taaggcaact agagaacaca
     6841 ataatacaaa tgtactttgt ttaggagaaa gagttattgg gaaaggatta gcaatgagaa
     6901 ttgttgaaga atggttaaat tcaagttata atggagaaag acatgcaaga agattacaaa
     6961 tgattgatga tatagaaaaa gaaaatttat aaatatatat aatatataat ttataacaag
     7021 ggggagcgtt atgtctatta tttgtgcttc aataatgtgt gcagaccaaa tgaagttaaa
     7081 agaggaatta gaagctcttg aagaagcagg tgtaaaatta cttcattgtg atgttatgga
     7141 tggaatattt gttaaaaact tggctatggg accagagtta ttaaaatcaa taagtgaaaa
     7201 tacaacaata cctttagaca tacacttagc cacagaaact ccagataaat atattgatat
     7261 gatgtcttat ataaagccaa aatatatttc ttttcatgta gaatcaagta caaatgtaaa
     7321 agctgatata caaaagttaa gaaattatgg tatagggcca gtgttagcaa taagccctca
     7381 aacaagtgtg gataaaatag aagaatatat aagtttagtt gagggtatct tagtaatgac
     7441 agtaaatcca gggtttgcag gtcaaaagtt taatctttct gtattggata agttggataa
     7501 acttacagag atactaaaag attatgataa tcctccattt atagaggttg atggaaatat
     7561 aaataaggat actattaaac ttatgaatgg taaaaaagta gatatatatg ttgttggtac
     7621 ttcagcttta tttaatgata agcctccaat ttcatataaa gataaaatag aagaactaaa
     7681 agaaagtata aagtaaccat gtaataaaat tataatatgt ttctgtttaa tagtaaaaat
     7741 ttaggtggaa acaagaaatt ttataatatt taataaaaat tttggttgag taaggagaat
     7801 tttttatggg agacctatat aaactacaaa atggtactga tattagagga atagcgtatg
     7861 aaaatgattc taaagaagtt aacctaactg tagaagaagt aaaaaaaata gctaaagcgt
     7921 ttcatatttg gctaaaagaa aagactaaga aagataaagt aactgttgcc ataggaactg
     7981 attctaggat aacaggaagc caatttagaa gtactgttat tgaaacttta actaatgatg
     8041 attgtaatgt aattgattgt gagattgcta caactccagc tatgtttatg acaactatta
     8101 tggatggata caattctgat ggggcgatta tgattacagc aagtcactta ccttattact
     8161 ataatggttt aaagtttttc acagaaagtg gaggtttaga aaaaactaat ataaaagaga
     8221 tgttggatat agctgttaaa aatgatagtg tagactatga ggaagtaaat aaaaaaggtg
     8281 aagttatagt aaaaaattta atagaagatt attctaattt attgatagat aaaattagaa
     8341 aaggtgtaaa ttcaagtaaa aattatgaaa agcctttttc tgggcttaag atattggttg
     8401 atgcaggaaa tggagctggt ggattctttg ctgaaaaagt tttacacata ctgggagcag
     8461 atacgacagg cagtcagttt ttgaacccag atggtatgtt cccaaatcat atacctaatc
     8521 ccgaaaacaa agaagcaatg gaaagcatat gtaaagctgt gttggataat aatagcaacc
     8581 taggaataat ctttgataca gatgtagaca gagctgctat tgtaggtaaa aatggcaaac
     8641 caataaataa aaatgctctt atagctgtta tatcatcaat agtattagaa gaacatccaa
     8701 aaacagctat agtaacagat tcgattactt ctgaaggact tgctaaattt ataaatgaat
     8761 tacaaggaag acatcataga tttaaaagag gatataaaaa tgtaataaat gaagctataa
     8821 gattgaatag tgaaggtgaa gagtgccatt tagctataga aacgtctgga catgctgctt
     8881 taaaagaaaa ttatttctta gatgatgggg catatttaat agctaaaatt ttaataaaag
     8941 tagcaaagct atccttggaa ggaaagacta tagaagaatt gatagaaaac cttgaagaag
     9001 ctcaagaaga gaaagaagtt agaataggca ttaacaaaga agattttaga ccatatgcag
     9061 aggatatatt aaaagattta gattctcatg ttaagagtat tgatggatgg tctgtagcac
     9121 caaataattt tgaaggaata agagttaatt gtgatgaatc aaatggagat ggatggtttt
     9181 tacttagaat atcactacat gaaccattac tagctttaaa tattgaatca aataaaattg
     9241 gtggagcaga acaaatttat tctaagttaa aacttttctt agaaaaatat gatttaaaag
     9301 gtctttaggg taaatagaaa gaatatattt atattaaaga acaaatgtaa atttgtacct
     9361 aagattacaa atattacaaa attgtaatct taggtaacag agatgtaact gattatggaa
     9421 acactatgtg atattataga tacagagaga tgaaatatgt gatgaattat agagaaatat
     9481 aatttaaaat gtattaatat cacaccaaaa aggtataatc aaaaaatatg taaatata
//
LOCUS       DACTGW010000152         9513 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.152, whole genome shotgun sequence.
ACCESSION   DACTGW010000152 DACTGW010000000
VERSION     DACTGW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.152"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            146..880
                     /locus_tag="I9573_002680"
     CDS             146..880
                     /locus_tag="I9573_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797290.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSVVIEKSDHEKSAVSLENFR
                     RIDLGEYVGVEESRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            1091..2272
                     /locus_tag="I9573_002681"
     CDS             1091..2272
                     /locus_tag="I9573_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAT4797291.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGEVFNKVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMIILNVKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGILGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGIHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVEIVI
                     R"
     gene            2277..2828
                     /gene="mobB"
                     /locus_tag="I9573_002682"
     CDS             2277..2828
                     /gene="mobB"
                     /locus_tag="I9573_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAT4797292.1"
                     /translation="MKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKH
                     TCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQLYEDMDLI
                     IIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLNDYKNISKY
                     IESCIKNEILSISNVEIKNILKE"
     gene            2947..3222
                     /locus_tag="I9573_002683"
     CDS             2947..3222
                     /locus_tag="I9573_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4797293.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIHAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            3478..5178
                     /locus_tag="I9573_002684"
     CDS             3478..5178
                     /locus_tag="I9573_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797294.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            5541..6728
                     /locus_tag="I9573_002685"
     CDS             5541..6728
                     /locus_tag="I9573_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797295.1"
                     /translation="MEAITAFCLVLISLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTIVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            6788..7951
                     /locus_tag="I9573_002686"
     CDS             6788..7951
                     /locus_tag="I9573_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4797296.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IEIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            8102..8905
                     /gene="zupT"
                     /locus_tag="I9573_002687"
     CDS             8102..8905
                     /gene="zupT"
                     /locus_tag="I9573_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAT4797297.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            9010..>9513
                     /locus_tag="I9573_002688"
     CDS             9010..>9513
                     /locus_tag="I9573_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HAT4797298.1"
                     /translation="MEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGIFYF
                     HKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYKDIP
                     PKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVLQII
                     LLILYAVQ"
BASE COUNT         3709 a          949 c         1744 g         3111 t
ORIGIN      
        1 acaattagct tcttaatctt attaaattca aataaatatt atataattat tttataatat
       61 aataaatata attattgaaa aaagagataa ctgtaaattg agatatagat aaatactata
      121 aaatttaaga aaggaggatt gatttatgaa gttaaaaagg tcattagttt gtgtaactat
      181 tttaggtatt atacttgttg gatgtcataa agaaaatact aaagagaaaa atcaagtagc
      241 aagtaaagca acacagcaaa aaactatgac aaaagttcaa aatgatgtta atgaaattat
      301 gaataaagat tataagtaca ttataaagaa tatgggtata ccttataata cattttatta
      361 tataaaacct aaagttttaa aagaatcgaa tacaatgcaa gatataaata catcaagtta
      421 tatgacatta gtttatccaa aatatactgg aaatgatgaa ctagatggaa gtgctttata
      481 tgttgatatt aacgaaaata aagttgtcaa tgttgaaacc aattctttct catcacaagg
      541 tatatcagtc attgatgctg aatctagtgt tgtgattgaa aaaagtgacc atgaaaaatc
      601 ggctgtatca ctagaaaatt ttagacgtat tgacctggga gagtatgttg gtgttgaaga
      661 gtctagaata aatgaaattg taggagatgc gaattatgat ttaacagcat ataatcatga
      721 aggtagtaaa gtagttaaga gttatagatt gaaagaagat aacaaaatat taaaaaaaga
      781 ggtacttaca ataagtattg tggataacaa aataaaatct ataaaaacta ttgaaagtga
      841 taaaattgta aaaataataa aaggaacttt attagaataa aatattctaa agcatatgat
      901 gctgtttcaa gatagaagga aatttctagc tgggaacagt ttttttatta gatttataaa
      961 atttgaactg cacttataga taactattag ttataattaa atataagata aaagtatttc
     1021 caacttttat aatctataat atattacata gaaatatctt aaataaaaaa tatattgggg
     1081 agtatttaaa atgatagaat tagaagatgc cataaaaatt attgaaaaaa atgttttacc
     1141 aataaacagg gtcaagatag taaatcttat agatgcagta aataaagtag ttgcagaaga
     1201 tatatattca acaattgata gtcctccatt tgataggtca ccctatgatg gttatgcata
     1261 taatgcaaat tctgaaaata gaaatttaaa ggttataggg actttgtatg caggagaagt
     1321 atttaataag gtattaaata aaaatgaagc agtgaagata atgactggtg gaaagatacc
     1381 acagggtgca aactgtgtaa taaaaaaaga agatgtaacg gttataggtg atatgataat
     1441 tttaaatgta aaattaaagg aatacgataa ttacatattt aagggtgagg atataaaaaa
     1501 ggggcaattg attattaata aaaatcagaa tataggatat gatggtattg gagtattagc
     1561 aagtcttgga atatccaaag taactgttta tgatgattta aaagttggaa ttttgaatac
     1621 aggtacagaa ctacaagata taaatgagac tttagagaat ggtaaaatat acgatagtaa
     1681 tagatatacc ttatattcaa gattattaaa acttggaata caggcagata caatatgcaa
     1741 ttgttctgat gatatattgg agttagaaaa aaaggtaaag aatatgttac agagtgtaga
     1801 ttttttaatt acaacaggtg gtgtttctgt tggagataaa gatttaattc cagatgtatt
     1861 caaaaaaata ggggcaacag aattattttg gaagataaac attaaacctg gtggagcttg
     1921 ttatgtaggt atattgggag aaaagctatt atttggtctt tctggtaatc cacatgcagc
     1981 aatagtggta tttgataatg tgatagttcc tgttattgag tatttaacat ataagaaggg
     2041 aatacatcat ataaaagcta tatttaaagg agaatttaat aaactttcaa ataaagatag
     2101 atttgtaagt ggaaaactat atacaagtga aggtaaattg tatgtagagc taaatgataa
     2161 gaaagatgca aaagctagat tatctgcaac tctaagaagt aattgtatga ttaagattaa
     2221 aaagaatgag acaataaaag aaaatcaatt agtggagatt gttattagat agttttatga
     2281 aaggacacaa ttttatgtat aatatattgt cagttgtctc atattcagga gtaggtaaaa
     2341 ctacgttgat tgaaggcata ataaaagaat taaataaaaa agggtatcgt gttggagtta
     2401 ttaaacatac ctgccatgat tttgatatgg atgaggaagg aaaagatact tataaacata
     2461 gaaaatctgg ggcgagaaaa gtctgtataa tatcagataa acgagtagca tatattgaag
     2521 aactaaaaga gaagaatcca ttatataaaa tgattcaatt atatgaagat atggacttga
     2581 ttatcataga aggatataaa aattatagat ttaaaagatt ggaagtaact agaaaaggta
     2641 agtatgagga tattttatct aataagtctg atttgatagg tattgtcagt gatatagaat
     2701 ataaattgaa tattgaacag tttaatttaa atgattataa aaatatatca aagtacatag
     2761 agtcttgtat aaaaaatgaa attttaagta taagcaatgt tgaaataaaa aacatattaa
     2821 aagaataatt ttaagatgtt cttcttatat aaaatgatat aattatttat ataaataaag
     2881 atataagaaa tttatcaatt ttattttgag ttataaaata ttaaagaaat tgtaggagga
     2941 caatatatga aaaaaatatt agtagcatgt ggagcaggaa tcgcaacatc aacaatagtt
     3001 tgtgacagag tagaaagatt ggttaaagaa aataatatac acgcagaagt tgagcaatgt
     3061 aaaattgctg aatgttcaac aaaacaagaa ggtgctgatt taatcgtatc tactactata
     3121 ctaccaacaa cttatgatat acctacaata aaggcaacag gatatataac aggtataaat
     3181 acaacagcat tagataaaaa gatattaaat gcattgaagt agttttgtgt ttataaaaaa
     3241 aatttagtat taaatataga gttgtatctt tataaaattt taaatataga gatatagctc
     3301 tttttatgct gtttcaagta tacaactata tgatataaat cttcaatttt atgaaaaaga
     3361 ttgttattac tttgtaagtt tataaaagat ttaaagttat tgttatgtta tactaattta
     3421 tgggtatttt ttagaagtac tatatagaag tactaataaa ggagggattt aatacttatg
     3481 ataaaacaat ttgtcaaata ttataagcca tataagaaaa tttttacact agaccttata
     3541 gcagcttttc tattttcctt atgtgattta gtttatccta tgataactag aaatataatg
     3601 gatgacgttg ttccaaataa aaacttaaga atgttagtag tttttgcagt agcacttata
     3661 cttatattta tagcaaaagc agggcttaac tattttatgc agtattgggg acatgttatt
     3721 ggtgtagata tgcaagctga tatgagaaat gaggtattta cacacttaca aagacttcca
     3781 aatacgtatt ttgataataa caaatctgga gtaactatgt ctagaatagt aaatgattta
     3841 atggatataa cagaacttgc acatcatggt cctgaagatt tattcatttc tatagtaatg
     3901 ttggttggtt cattctttat acttatagat ataaatattc ctcttacgct tataatattt
     3961 gctatattac cgtttataat ttggtttgca atagctaaaa aagataagat gaatattgct
     4021 tttatgaaga gtagagttac tataggtgat gtaaatgcta ctttagaaaa tagtatagca
     4081 ggtatgaaag taactaagtc tttttgtact gagaaagaag agttaaataa gtttgtaaga
     4141 agcaataaat tatttagaag agcaagacaa gattcttata aggtaatggc agaatattac
     4201 tctggtatga atttatacat ggatatctta gagtgggtag ttgtaatagc tggtggttat
     4261 tttacttata taggaaaaat tactttgggt gattttgctg cttatatact atatgtaaaa
     4321 atgtttatac aacctatgaa aaaattaata aattttacag agcaatatca aaatggtatg
     4381 acaggtttta aaagattcat agagataatg gaacaagacc atcaaaaaga agctaaaaat
     4441 cctattgaac ttgaaaatgt aaaaggtgac attgagatag aaaatatatc tttcacatat
     4501 gaagataaga ctcaagtttt ggataattta agtttatcta taaaagctgg taagactata
     4561 gcattagttg gaccttctgg aggaggaaag actacattat gtaatctatt gccaagattt
     4621 tatgaatttg ataagggtga tataaaaatt gatggaaaga gtataaaaga tgttagttta
     4681 aaatcattga gaaaaaatat aggtatagtt caacaagatg tatttttatt tacaggaact
     4741 ataagagata atattctttg tgggaaccct aatgctacag atgaagaaat gatagctgct
     4801 gctaaaaaag ctagaataca tgactttgta gagactttgc cagatggata tgatacttat
     4861 ataggagaaa gaggcgtaaa attatcaggt gggcaaaaac aaagaatttc aatttcaaga
     4921 atctttttaa agaatcctcc aataataata ttggatgagg ctacatctgc tttagacaat
     4981 gtaacagaaa gagaaattca agagtcttta gaggaattaa gtaaagatag aacaaattta
     5041 gttgttgcac atagattgac tactataaaa aatgctgatg aaatcatagt attgacggat
     5101 aaaggaattg aagaaagagg tactcatgaa gagttagtaa acaaaaatgg agtatatagt
     5161 aggttacaca ataactagta tacaaatttt acaccatagc taacatacgc attaaactta
     5221 caaacatagg atatattaag attcataaag acaattcatt ttatatttat gagatgtttt
     5281 atgaataaaa tgtatcctat gttttatttt ttgcaaatta catttaattc tctttgtaag
     5341 aatagagtta aatagccaat ggtcaggata acgtttttac aaaaagaata atttagaaaa
     5401 ctaactttaa tttatggaat ttatcgaatt ttatgaaaaa aatcgaattt tgacttgaaa
     5461 tgtttttcaa aagtagtaaa ataatggata actatacata cattttataa aaatacattt
     5521 attatgaggg gggattttgt atggaggcaa taacagcttt ttgtctagtt ttaatatcac
     5581 tgacgatagg agatatagtt tcagcaaaaa ctaaagcttt tgttccgtcg gtttttgtat
     5641 cagcaattat atttatagta ggattctgga cttttttccc agaaaatatt gtggacttag
     5701 ccgctttagg aacaccatta gctcaattag gtatgctttt gttaataact catatgggaa
     5761 ctatgatgag tattaaggaa ttagcaggtc aatggaaaac aatagtaata gccttggcag
     5821 gaatagtagg aatatgtgta ggtgctttgg cattagggac aatagtattt ggatgggata
     5881 cagcagtaat agctacacct cctcttactg gagggctagt tgcatctata atgatggcag
     5941 atgctgctac agcaaaaggt cttgcaagtt tatcagtact tgcaattttg atgtatgttg
     6001 cacaaggatt tgcgggttat cctattacag cattaatgct taaaaaagaa ggaaaaagac
     6061 ttttatcaga ctttagaagt ggaaaagtaa ctgtaaatac agatgaagaa aaagttaagg
     6121 atttacctga acaaaaaagc agatttaaga taattccaga cttaccagaa aaatatgata
     6181 cgacatatat gataatactt agattaggat ttgtagcatg gttagcagta caatttacaa
     6241 atcttacaaa tgaagttgta tcaagatatg ttatatgttt aatatttggt gtaatagcat
     6301 cagaacttgg aatagttgat agaaaaccac ttaataaatc tggttcattt ggatggttaa
     6361 tgacatcatt gatggctttt atatttgcag gattagcaaa agctacacca gcaatgcttg
     6421 ttgaaatggc agttcctctt gtaggaatca taatatttgg tgtaataggt atgggaatat
     6481 tctctattat agtaggtaag cttttaggtg aaagtaaaga aatggctttt tcagtttcgc
     6541 ttacatcact ttatggtttt ccaccaaact atatattaac tgaagaggca gtaaaggctt
     6601 tggctgaaac accagaagaa aaagaatttt taatggataa gatgcttcca aagatgcttg
     6661 ttggaggatt tactacagtt acaatagctt ctgtagtaat agcaggtatc tttgtaaatc
     6721 taatatagta tttaagtgat aaaaagtatt ttaaataaga agaatgtgta ctacttggag
     6781 gtatggaatg gatattaaag aaataacaaa gagttataaa gactatgtaa ttaagcttag
     6841 aagggaattt catgaaaatc ctgagaaaag tatggaagaa gttagaacat caaagagagt
     6901 taaagaagaa cttgataaaa tagagatacc atatgtatca gctggaggta caggtgtaat
     6961 tgcaactata aaaggagcta atccaggaaa gactgtagca cttaggggtg atatggatgc
     7021 tctacaagtt gttgaatgca ctgatgtaga atacaaatct aaaaatgaag gacttatgca
     7081 tgcttgtggt catgatggtc acacatcaat gcttctaggg gcagcaaaag tgttaaatga
     7141 tattaaggat agtatcaatg gaactgtaaa attatttttt cagccaggag aagaagttgg
     7201 taaaggtgca agagctatga ttcaagatgg tgctatggaa ggtgtagata gtgtgtttgg
     7261 aatacatctt tggactgatg tagaatctgg gactatatcg gtagaagaag gaccaagaat
     7321 ggcttctgcc gatttcttta aaattactgt aaaaggtaga ggtggtcatg gttcacttcc
     7381 tcatcaaggt gttgatgcag tgcttgcaag ttcagcaata gttatgaatc tacaatctat
     7441 ggttagtaga gaagtaagtc ctctggaacc attagttgtg agtgttggag ttttaaattc
     7501 tggaactaga tttaacgtaa ttgcgagtga agctgtctta gaaggtacaa taaggctatt
     7561 taatccagag cttagaaagc aaattcctgg aattttagaa cgtatagcaa aatctacagc
     7621 agaagcatat agagcagatg ctgaattaga gtatgggtac ttaacaccag cagttataaa
     7681 tgacaaagaa tgttcaaaga tagctacaga tgcagcaatt aagctatttg gagaagactg
     7741 tataacacta tttgaaaaag taacaggagc agaagattta gctgagttta tgaatatagc
     7801 acctggtgca ctagcatttg taggagctag aaatgagtca aaaggtgctt gctatcctca
     7861 tcatcatgga tgctttaata tagacgaaga tgctctagaa attggaactg ctttatatgt
     7921 gcaatatgca gtggatttct taaataaata attttaatat actataaaga aaaagcagag
     7981 actaagttta tatctctgct tttttattgt gtatattttt tataaatatt gactatataa
     8041 aatatatatg atagcataaa gaaaatgata atcaatatca attaaaaaga aggtgattta
     8101 tttgaataat gtaatatttg catttttgat aacattattg gctggtttat ctactggtat
     8161 aggaagttgt atagcatttt ttgctaaaaa aacaaatact aagtttcttt ctataagctt
     8221 aggattttct gctggtgtta tgatttatgt ttctatgata gaaatttttc ctaaagcaca
     8281 agatgctttg acaaaatcta tgggagagaa attaggaagt tggtcaactg taatagcttt
     8341 ttttataggt atggctatca ttgctgttat tgataagtta attccacaag aagaaaatcc
     8401 acatgaaata aagaaaatgg agaatataga agaaaagaat atcaaaaaaa ataagtctct
     8461 tttgagaaca ggtatattta cagcaatggc tatagcaatt cacaattttc ctgaaggatt
     8521 agctacattt atatcagctc ttgatgatgt tacaatagct atcccgatag caattgcaat
     8581 tgcaatacac aatataccag aaggtatatc tgtatctgta ccagtttatt atgccacagg
     8641 agataaaaag aaagcttttt attattcctt tttatctggt atgtctgagc ctttaggagc
     8701 gattattgga tatgtacttc ttagaaattt tttaaatgat ataactcttg gaattgtatt
     8761 tgcaattgta ggaggaatta tggtattcat atcactagat gaattattgc catcagctag
     8821 agagtacggg gaacatcatt tatctattta tggattgatt gcaggaatgg gtgttatggc
     8881 tattagttta ttactattta aatagctttt taaatatatg attgtaacct aaaattagta
     8941 tcaaaatgat ataattattt aaatagattt ttaagctatt taaaaatatc attttggaga
     9001 atgagtgcta tggaaaagaa aataaaattc aaatttgact ggtatagttc acctgataag
     9061 atagagaaat ggttagaaga tattgaagct aaaggatata atttatataa gataaataaa
     9121 tttggaggaa tattttattt tcataaggga agtccaagaa aaataaaata cactgttgat
     9181 tactactatg atgtagagaa taattatttt gatgaacata aatcaaatgg ctggaatctt
     9241 gtatttagga gtattggtag ttttatgggt acatctgcat ggtatatatg gtcgaaagaa
     9301 tacaaagaca tacctcctaa attatatagt gattctgata aaaaaaacaa agtaaagtca
     9361 attttattta tgaatatgtt tatattcttt atgttagtag taatatttat atatttattg
     9421 aatattgaaa taagaaattt atatagtggt tatggagcat ctgtaactgc tattgtacta
     9481 cagattatat tgttgatttt atatgcagta caa
//
LOCUS       DACTGW010000153         9437 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.153, whole genome shotgun sequence.
ACCESSION   DACTGW010000153 DACTGW010000000
VERSION     DACTGW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.153"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(51..836)
                     /locus_tag="I9573_002689"
     CDS             complement(51..836)
                     /locus_tag="I9573_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HAT4797299.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDGLEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            complement(951..1910)
                     /locus_tag="I9573_002690"
     CDS             complement(951..1910)
                     /locus_tag="I9573_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAT4797300.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDIKRFKAII
                     ATSLVLGVLGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            complement(2068..2385)
                     /locus_tag="I9573_002691"
     CDS             complement(2068..2385)
                     /locus_tag="I9573_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797301.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            complement(2492..2995)
                     /locus_tag="I9573_002692"
     CDS             complement(2492..2995)
                     /locus_tag="I9573_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4797302.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            complement(3047..4246)
                     /locus_tag="I9573_002693"
     CDS             complement(3047..4246)
                     /locus_tag="I9573_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HAT4797303.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEENKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            complement(4506..4694)
                     /locus_tag="I9573_002694"
     CDS             complement(4506..4694)
                     /locus_tag="I9573_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797304.1"
                     /translation="MNYHTKEELMEVLRVASSRIINCEKVQKKFSEETSHHTRFKNII
                     EAMYISKSLIMDEISKRD"
     gene            5029..5997
                     /locus_tag="I9573_002695"
     CDS             5029..5997
                     /locus_tag="I9573_002695"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAT4797305.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTKL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPNTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLRECIALSKTL"
     gene            complement(6114..7244)
                     /locus_tag="I9573_002696"
     CDS             complement(6114..7244)
                     /locus_tag="I9573_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4797306.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDINISDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(7397..8350)
                     /gene="msrA"
                     /locus_tag="I9573_002697"
     CDS             complement(7397..8350)
                     /gene="msrA"
                     /locus_tag="I9573_002697"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAT4797307.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRIEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEVLGYGEYLKLFE"
     gene            complement(8474..9379)
                     /locus_tag="I9573_002698"
     CDS             complement(8474..9379)
                     /locus_tag="I9573_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin"
                     /protein_id="HAT4797308.1"
                     /translation="MKSSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
BASE COUNT         3188 a         1568 c          875 g         3806 t
ORIGIN      
        1 aataatctta taaataccat tattctattc gtcttcatta aattgtataa ttaccttttc
       61 tcattaataa tttttttaaa atgttctagt tgctcttgtt gttttttatg ttttatatgt
      121 ggaaatgctt ttataactct tttttgtaaa ataatattag actctatacg gtccaactta
      181 ctatttattt tgtttatttt ttctctaact tctaatccgt cgttatttat tgttttaaat
      241 aaagcttgta tttctcctct atcaaattta tccaatttca catttatatc attaaggtct
      301 gtaattatat cgtctagtaa gtttaacttt atatctaatt ttttaagtgt tataatagta
      361 acaatcaaat caagtatagt ccaaagtgtt gtaattatag ccattgaaat taatattcta
      421 tatggaatta tacttacaaa atattttata aatggatgta taaatcttat tattaaaaca
      481 gacattacac caaagtatat tgcatttttt aaacatactc taccctttat attgaatttt
      541 ctgtcagaat aatcccacca tttagcatga aataaggttt ctaaaataaa tcctgtaaaa
      601 tattctagta tcgtagttaa aatcattcct aatagaaata ctattactac attattaaat
      661 cttcctaata gtgatatcac aattactgct ccaaaaccat aaacagggca tattggacca
      721 tttaaaaaac ctctgttgat tattcttttg cttgctatag aacatcctat actttctact
      781 gtccatccta aaaatgaata tataaaaaag tacaagatat attttattat ctccataatt
      841 tatctctact ccttcactaa tgttcttatt ttatgcacaa cgtaaaaata attatagaaa
      901 tagttttttc gttttttata tttgttattc ttttattctt gtttaatatt ttattcatac
      961 aatccaatta ataatttact agccataata ccatgtttat cctttggctg attttcttta
     1021 tcaatccaaa cattaatagt atcatatctc cttaataaat ctaaattctt cacatcaaat
     1081 tcctcatatt taactatatc catttttgat tctttggtaa ttatataaga tactaatttt
     1141 gtattttcat ttaaataaat tcttatattt ccatcagaat caattaaatt ctctggtctc
     1201 tttactttgt tattagaacc tgctttaata gttattattc cttttttaaa gctctcaaat
     1261 gtcccaacta tatctactgt atctttattt atatctttaa ttatttccct attttcttca
     1321 attaatattt ttgatatttt tctatcaggt ctaaatttaa aagttagtaa gttatgataa
     1381 tttttagaaa taactcctaa tcctaaaact cctaaaacta aactagtagc tattatagcc
     1441 ttaaatcttt taatatcatt aggaaataat ttattcatgt ttatgtaaat attaattcca
     1501 attattccac ctaatgaatt taaaataaca tcatctatat cactacttcc aagagcaaaa
     1561 atatattgtg ttaattcaaa tgctaggcta acaagtataa ttactataat tatagttttt
     1621 ttatttagat ttttacatac tataggtaaa aaaattccca taggaataaa tatacctata
     1681 ttttctataa tatttacagt agctttcata aagtcaagat tactatctac tataaattga
     1741 tatattgtat aaaatggaat taaatttaca ttcctaaacg ttcccttgaa aatagcgtaa
     1801 attggtacat ctctaaataa aattattctt ataatcatta aaatatatac caaaaatatt
     1861 atgtatataa ttgacagtaa caatttttct gactttttac tttctatcac tgataagtct
     1921 cctttcgcta ttaatatttt aagtatatca ataaatattt aagacgattt taaaatttcc
     1981 ttaaaatttc cttaattttc aaacaaaaaa tcaaaaaagc ctacacttaa atgtgtagac
     2041 tttaataaat tttcatacaa atacatttta ttgaataacg aacaatctta atgtagaaaa
     2101 aactataact acagttaaaa gaagtctagc atataaaaaa tttttttctt tcaacataac
     2161 tcctgcaact ccaaatataa aaaatagaga agcaataaat tttccaataa gattattatt
     2221 aggataataa ctaaaaagca taaaacaaag tacaaatgta agtactccta ttgcattaaa
     2281 cacaatttta ttagatgcta tttttccttt attgaaaaag aaacagaata ttgacactaa
     2341 atctaaaaat aaaaatacat aaaacattcc ttttaaaacg cccattttta taccccctaa
     2401 aaattctaat tataatatcc agtttatttc atataatcgt caatataatc aatactttat
     2461 aacatcagta taacagtatt acaaatttat attacgaatg tattacttcg tattcataca
     2521 tattataatc acactcatac atatttaaag actcatatgt tgcttttgca tttatgtttt
     2581 ctttttctac atataatctc attccaacta tattttcatc tttatcacat atatttttta
     2641 tataattaaa taaataatta aagatacctt ttcttctata ttccttatct acatatacac
     2701 tctgaatcca aaggaaattt ccatttctcc aatcactcca ctcatatgta tacataatct
     2761 gtgcaacaac tttatcaaat actgtatata catgatattt tccttttctc tcatctaata
     2821 acaatgcttt tacaccttta gttaaaacat ccatatcaag ttcttttcct tcagtttctt
     2881 tagccaaatt ataattaaat tttgctattg tatccaaatc atccatacgt gcttctctta
     2941 tctctattag ttcttcattt tcatataact ctatgtagtc cttttgttgc tccatactta
     3001 ttcctccata tctattttat ctactaaggt tttttatata atatatttat agagtttttt
     3061 ctatcttcat aaatgcataa tcaagtgtag cctctaaatt tccattattc ttacctcctc
     3121 cttgagctaa tactttactt ccaccaccct tgccatctat aagacttatt gcatccttta
     3181 ataaaactcc catattaata tcttttaaat ttttagaaca tgcaaaaact aaatttattt
     3241 tatcattaaa tttaacagct aataatgcaa tactattttc atattctgca attttatttg
     3301 ctatcttatt tacatatttc aaatcttcat catcataaat ttttttaatg accgaaatat
     3361 tgccaacctt atttgctttt tcaatcattt ctttgacctg atatgtagat actacttctt
     3421 caagccttct ttttttaatt aaagcctctt ccactttact atttagattt acaataccat
     3481 ttatagcatc ttcctcacta cagcttaaat atctacatat tttagtcaaa tatatatccc
     3541 tcttcaatac ttcatccact gcccttttac ctgctagaaa ctctattctt gtacttgact
     3601 tatgtttttc ccatttcttt atttttatca ttctcaattc accagttgat tttggatgca
     3661 ctccacaaca tgcattcgta tctaaatcac ctatttttat tactcttatc tcttcatcag
     3721 tatttggcaa atcacgtctt aaccaaatct tttttagttc ttttttcgtt ggaagtaatg
     3781 tctctacttg tatattttgg cgaataattt cattggcaaa caactctatc tctctaattt
     3841 tgttctcttc taaattacct tctatatcta ctgtactata ctcatttcct aaatgaaaac
     3901 ctgtcgtatt tgcattaaaa gttttaaaaa aacatccaga taatacatgt tgtccaaaat
     3961 gttgatgcat tccatcttct cttctatccc aatcaatact acactttacc ttatgtatct
     4021 ttataggttt tttatctaat acatggtata tattttttcc atcttcatac acatttatta
     4081 catcatcatt ctctattttt cccaggtctc caaactgacc tcctccacca ggaaaaaaag
     4141 ctgtcttatc taataaaaca tgatacttat tttctacttc ttttatttca attatttctg
     4201 ctgtaaaatc tcttatatac tgatttgtat aatataactt ttccattata attctatctc
     4261 cattttctta attatagact actttacttt ttaaatacaa atttcaagct aagcttaatt
     4321 taattgtatt aaaatagaac tcatcctatt atttactaac atcttctact atattttaac
     4381 atatagatat tagtgttaac aaatttataa ttttatatta tttcaatatt ttataacaag
     4441 tatgcttact tattttctta tataataaaa tgtatattaa aacatattaa attgcaaaat
     4501 aagcttcaat ctcttttact aatttcatcc attattaatg attttgaaat atacattgct
     4561 tctataatat tcttaaaacg tgtatgatga gatgtctcct cagaaaattt cttctgtact
     4621 ttttcacaat taataatcct tgaagatgct actctcagta cttccatcaa ctcttctttt
     4681 gtatgataat tcataacaca tttactcctt tgttttttaa tttagagaaa cagtatacct
     4741 caatttaata tcattatcaa ctgatatcat tattatttat atatatcttt taacacttaa
     4801 atattctaac tatcagtgac attacatatt ttaataatta ttaatataca tataatactt
     4861 taacattcat taatagagag tatattaaca ttgctttttt aaaaatttta gtataaaatc
     4921 atattaaata aattttctga aataataaaa tattaaaata tccaaaaaca gcttttgata
     4981 acatctataa ttttcaatgt atcgttattt tagggggagt gatttaaaat ggcaataaag
     5041 ccaagaaaaa tatctattgt aggttcaggg catgttggtt ctcattgtgg atttagttta
     5101 ataactcaag gagtttgtga tgaactcttt atgattgata tagatgaatc taaatcaaaa
     5161 gctcaagctt tagatttagc tgatgcagtt tcatatctac cacataaagt acatatagaa
     5221 aaaggaactt tttctgactg taaagattct gacatagtag tgataagtgt tgcagactct
     5281 tcagaagggc ctttacgtag acaaaacaca actagattag atttactaag accaactatt
     5341 ggaatgataa aatctatagt aaaacccata gttgattctg gctttgatgg tatatttgta
     5401 gtcatatcta atcctgttga tgtagttaca aattatattt gggaaaaatc tggctttcct
     5461 aaaaataaag tcattggaac tggaacagct ttagattcaa caaaacttag aaggatttta
     5521 tctgaagaaa ctggaatcgc tcaacaatca attcaagctt attctatggg tgaacatgga
     5581 gattctcaaa tggttccttg gtctcatgtt tcaattggtg gtaaaccaat cttggacatg
     5641 ataaaagaca acccaaatac ttatagcaat ttagacttac catcaatagt tgaaaaaaat
     5701 aaaaaaacag ggataagtat aataaatggt aaagattgta cagaatttgg tataggtact
     5761 gctttagtag aaattgtaaa agctatacta cacaatgaga aaaaagttct tccagtttct
     5821 acattactag aaggtcaata taacgaaaga aatgtatttg caggtgttcc atgtgttata
     5881 ggaaaagatg gtattgaaga aattattgaa atcaatatga ctgaatatga gcaaaacgaa
     5941 tttaataaat cttgctctgt tttaagagaa tgtattgcac tttcaaaaac actttagatt
     6001 caatattctt tcttacacta aaagcaggta aatgacttat tgaaaagact tttaactaag
     6061 ttaatgttta aatcatttat acctgctttt aattttaata ctaaaatata aacctatttt
     6121 ttaccatcaa attttataac tttacttttt ttatttacct cttgagataa caatcctttt
     6181 ttcaatccca tcaataattc taagtcttct ggataaagat ttattccaaa ttcctctaaa
     6241 cttgatacta tactatttac actcattatt ttattttcta tcaataattc aactgctctt
     6301 tttaaatacg ttgggcttga acctttcatt ttgtctagtg gttcttctat caaccagcct
     6361 tttttatcca tttcattcat aagataattg tattttttgt aatttatctt ttctagatta
     6421 tatgctctaa aaatcatagc agatataggt acaatccatt ttttctttag ttctacataa
     6481 taatctaaat cttctggatg agtcaaatct tttaaaaaac tctcttttgg caataaaaat
     6541 gcacaggcat actcatcttc tgaaaattgc ttacttggta ttcttaattc acttgatatt
     6601 atatatccaa gctcacatgc caaagtataa tttctaattg atgctgactt tttgtcatta
     6661 cccagagata ttagatattt agattcttta ctaattctct gtttttgagt aaagatagtt
     6721 gccccttttc tatctacatt catacctgat attataatcc ctttagattc taacaaactt
     6781 accatattta ctattggagt actatttaaa tcccaaaact ctctagtctt atatgcaaga
     6841 gattctatat cacttatatt aatatctgac attacttttc tatttggcaa gttttctttt
     6901 ggaaacttaa tataattctc cataaatgaa tatattttat gtattataat tattttttct
     6961 ctataagaaa tctcttctac tctaggtagt ttagattgag ggttaaaatg gctatcctct
     7021 acaacgatat ttatattttc atttttataa aaatactctt ttggaaaatt taatatatta
     7081 gataatttta atgtattttc tatatttggt acatacttat tttcctcaaa agcttttaaa
     7141 tcttttttat ttattttagt ttctttagac aactgttcta tcgtctttgc tctaaaaatt
     7201 ctagctgatt ttaatctctt tccattaaag tcttttaaat tcatttgttt tctctctttc
     7261 aaaattaaat tcttaatcta ttggtataaa taagattaca attaataatt tatcatatta
     7321 ttgacaaaaa aacaaaaggt gaaaatattt ttttatattt tcacctttta gataagttta
     7381 taataaatat tcctaattac tcaaaaagtt ttaaatattc tccatatcct aaaacttcca
     7441 tattttctct tggtataaat ttaagagaag cagaatttat acaatatctt aaacctccaa
     7501 gttcttttgg accatcatca aatacatgac ctaaatgaga atttccttta gcacttctta
     7561 cttcaattct gtacatacct tgtgagaaat cacttctctc aatgatagat ttatctataa
     7621 ctggctttga aaaacttggc cacccacatc cagaatcaaa tttatctttt gaagaaaata
     7681 acacttctcc acttacaata tcaacataaa ttccctcttt gttattatca taatattcat
     7741 tttcaaaagg tacttctgtc atatcatttt gagtgacttc aaattgtata ggagttaaag
     7801 tcttttttaa ctcttcccta tttatattgt ttccatccca atgacttttt acaaaattat
     7861 atcttccaga ccctttataa tacatattgt agtttaatgg tcttttttta taataatcct
     7921 gatgattttc ttctgcttcg tagaatttag ttgctggaat tatatcagtc actattttac
     7981 tatcaaatat tttactattt tccagatgtt ctcttgactc taaagctata tttttttgtt
     8041 cttcatcatg ataaaatata gcagtcttat attgagttcc cctatcacca aattgtccac
     8101 catcatctgt aggattaatc tgtttccaga agatatttag aagttcatca taacttatta
     8161 tatcatcatc atatgttatt tgaattgctt ctaaatgacc agtagttcct gaacatacct
     8221 cttgatatgt agggttttca acatgaccac ctgtatatcc agaaactact ttaataactc
     8281 catcatactt atcgaaaggt tttaccatac accaaaaaca tccacctgca aaagtagcaa
     8341 gttttatcat tttcatcact ccttaaataa attatacttt atttattccc taagaagtaa
     8401 cttaataatc tgcattttat tatttttacc tactcaatga aatcatgtgc aaaaatacaa
     8461 ttgcaaattg aatctaattt ctataaaagc tctttttctt tatccatcca aaagcatctt
     8521 ttatatcaca attttctcct aaagacatta gatattctaa tgcatcacaa tcatccatag
     8581 ctacatcttt ttccatctta ggatttaatc tatagtaatt atctccaaga agtttttgag
     8641 aaaataactg actcatttga gaatttcctt ctaaggtaat agatataatt ggtaaatcgg
     8701 gttctttatt aattatccag tcaatagctc cccattcagt agtatctgcc tttatactat
     8761 tatatacata tccagtccct attgaaaaaa gtcttgtatt cttaagttta aatccactgt
     8821 caatggcatg aattatacac gctaaacttg ggtcagttgc tattattcct ccatcaacat
     8881 gacgattata tgttggaaaa aatactggag cagcactact tgccattgct acatctacaa
     8941 ctttgctagt ctttgtaaaa gaatttggca tattgttata aaacactggt ttccatgcat
     9001 tttcctcatt tcctatataa aaacttggta tcattactaa tttatttaaa tcttttaatt
     9061 ctagttcctc tggaaatata cttaataaaa cttcttttaa gtttttgttt tcatactttg
     9121 gtctgagtat ttctgcataa cttttattaa aaatatactt agaattttca attgagtaaa
     9181 gttcttttat ctctttaggg cttacaccat acgcaagtcc tagtgcaatt agactccctg
     9241 tagatgtccc tccaaacata tttatattgt ttaatagatt tggatactct tttactatcc
     9301 tctcaagtat ggatatactc aatgcccctt ttaatccacc accatcaaat gccaatatat
     9361 taaaattaga acttttcata tacatatacc cccacataaa acaccttata ttttaatcaa
     9421 aataatttca ctatagt
//
LOCUS       DACTGW010000154         9246 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.154, whole genome shotgun sequence.
ACCESSION   DACTGW010000154 DACTGW010000000
VERSION     DACTGW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.154"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            283..417
                     /locus_tag="I9573_002699"
     CDS             283..417
                     /locus_tag="I9573_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAT4797309.1"
                     /translation="MDLINTIDKVQQAHKPIDEIIKTIRDIVDIIKILEPIYNFKSNQ
                     "
     gene            922..1683
                     /locus_tag="I9573_002700"
     CDS             922..1683
                     /locus_tag="I9573_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HAT4797310.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNKKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            1748..2530
                     /locus_tag="I9573_002701"
                     /pseudo
     CDS             1748..2530
                     /locus_tag="I9573_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            2797..3216
                     /locus_tag="I9573_002702"
     CDS             2797..3216
                     /locus_tag="I9573_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HAT4797311.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERIFNLKQEKRKAKHKGH"
     gene            complement(3319..3585)
                     /locus_tag="I9573_002703"
     CDS             complement(3319..3585)
                     /locus_tag="I9573_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAT4797312.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            3812..4513
                     /locus_tag="I9573_002704"
     CDS             3812..4513
                     /locus_tag="I9573_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797313.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            4595..5203
                     /locus_tag="I9573_002705"
     CDS             4595..5203
                     /locus_tag="I9573_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAT4797314.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            5527..5991
                     /locus_tag="I9573_002706"
     CDS             5527..5991
                     /locus_tag="I9573_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HAT4797315.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            6628..7644
                     /gene="gap"
                     /locus_tag="I9573_002707"
     CDS             6628..7644
                     /gene="gap"
                     /locus_tag="I9573_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAT4797316.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYSDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNQKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            8039..9208
                     /locus_tag="I9573_002708"
     CDS             8039..9208
                     /locus_tag="I9573_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797317.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
BASE COUNT         3748 a          920 c         1616 g         2962 t
ORIGIN      
        1 aaaatcaata cttgaattat tatttttatg tgaaatttat ctatagtata tttttataaa
       61 caagtaatgt tattaaaatt aagtatatag ttaaaagtgt atagcaaaaa ggctgttata
      121 ttaatgttag aggttcaggg aaaatatata aagcaaagat atttacagat aatgtagatt
      181 gcacagctat aagttagata atagaattat gtaatcaaga atttacaaca aagtataaga
      241 taaggataat gctaaattgc catacaggtg ctagatgtgt aaatggattt aattaatact
      301 attgataaag tgcaacaagc acataaacca attgatgaga ttattaaaac tataagagat
      361 atagttgaca taattaaaat attggaacct atttataatt ttaaatcaaa tcaataatga
      421 ttaagattaa aataatatta aggtatcaat gttataagag tacaatataa ggagtactat
      481 atagtactcc ttacttatac atattaggca ataggaaatc ttttcatagt taaattttca
      541 aatatttttt agggaacttt ataattgaga attaagggta tagtatgtga tgaacttact
      601 aaatttataa ttaggttata taatggaata atcatcaata gttttacttt agggaaaata
      661 tatgcttaag gaagtatagc ttactaatat tattaaaatg tatatttaaa atgataaaaa
      721 aagaattaga caagtattta gtaaattgat tatgtaaaat tctatcaagc aggaacccta
      781 tgactttagt catgggaggt tcagtaggta acttgttgaa ttaagtttat attatttaaa
      841 taaactactt aatttagata atataaaaag tcagaatttt aagaatagag ctatataatc
      901 ttagctatta ggagaagtac tatgatagga atatatttta gtggaacagg aaattcaaga
      961 tactgtgtag aaaaattttt acaagaatat gatataacag caaattctta ttctattgaa
     1021 gataatgaac ttttacagca catcaataat catgagaata ttatttttag ttatcctgtg
     1081 caatatagta atgtgccaaa aatattgaaa gattttataa taaatcattc aactttgtgg
     1141 aatggtaaac gtgtttttat tattgcaact atggctctat ttagtggaga tggctctggt
     1201 gtgttagcta gaatattaaa aaaatacggt gctataactg ttggtggatt acatgtgaag
     1261 atgccagata gtataggaga tgaaaaggta ttaaaacatt ctttagagta taataaaaag
     1321 cttgtaataa aagcagaaaa gaaagtaact aaagctgcta aaaaactgaa acatgggtca
     1381 ccaccaaaag aagggttagg ttttttgagt catattgtag ggttatttgg acaacgattg
     1441 tattttatca ataaaacaaa tatgtataca gataaactaa aaattaatac tgaggaatgt
     1501 attgtatgtg gtaaatgtgt taatttatgt ccaatgaaga atctagtaat taaaaattgt
     1561 atgattgttg caaatgggca atgtacaatg tgttatcgtt gtgtaaataa ttgtgcaaaa
     1621 aaagcaatta cattgttagg taaaaaagtt gttgagcaga gcaatattaa aaaatatatg
     1681 taagaaattt aaagttttat tgaatgtaaa ttgatgtata ttttatgttg gaaagagagg
     1741 tcaaataatg gaatactaca tagttgattc atttgcaact aaactattta aaggaaatcc
     1801 tgctggagtt tgtgttcttg acagaataat acctttagag ttaatgcaga aaatagcaga
     1861 agaaaacaat ttaccagaaa cagcatttgt agttaaaggt aaaggaaatt atgaattacg
     1921 atggtttact cctaaagcag aaattgattt atgtggtcat gcaactttag cagcagctta
     1981 tgtaatatca aattttattg atgttaatgt aaaaaggata gatttcttta ctcaaagtgg
     2041 aaagcttgaa gttacacgaa atggaaatct atatgaaatg atatttccag aaataatgcc
     2101 tattgaaata gagttatcac cataacaagc taatttgata ggctgtgtac caagtgctgt
     2161 ctattcttca agagatttaa tcttattgct aaacagtgaa caagaagtta taaattataa
     2221 acctaattac gcacaattgc gtaaattgac agactggtta ggtataataa tcacaccaca
     2281 aggaagtaat acagattttg tatcaagata tttttgtcca gaattagatt cagaagaccc
     2341 tgttactggt tcatcacatt gtaattttat tccatactgg tctgaaaagt tgggaaaaca
     2401 caaaatggta gctgctcaac tttctaatcg tggtggtata attcagtgtg aagtgttgaa
     2461 ggataatact gtaaaaattt ctggtgaagc agtcttgttt atgcagggaa caataaagat
     2521 tgatatttaa ataagtataa taactaattt attatgaaaa ataattaatt ttcttagtgt
     2581 taacaataat aaagtagagt taaaaagctt gatttcactt aaatcaatat gatagatgtt
     2641 gatactaaga ttgacaagca tgattaaaaa agataatatt taatctataa agataaaaac
     2701 acagtctggt tggtagtcca gacgcagcaa tagctgtcag taacctgcct ccttggttgt
     2761 ccgttcttta gaaagtaatt taaggaggaa ctagcgatgg acaaaattag tggaaaatta
     2821 acagtcttat ttgaagaacc attttgggta ggtatatttg aaagacaaga tggtaaaaaa
     2881 tatgaagcat gtagggtagt ttttggagca gaacctaaag aagtagaagt gtatgaattt
     2941 atacttgaaa gatttttctc attagatttt ggtagcatta aattagaaaa aaatgtgacc
     3001 aaagacaaca ttggatacaa aagaatgcag agaaaagtaa aaaaggaaca agaaaaagaa
     3061 actataggga ctaaggctca aaatgctctt aagcttcaat atgaagaaag aaaacaggat
     3121 agaaaaaacc tagctaaaaa tagaaaagaa gaagaaaaag aaagaatatt taatttgaaa
     3181 caagaaaaga gaaaagctaa acataagggt cattaatttt aattaaatat ctttttaata
     3241 attgctagaa aaaggtgtat aaagttttta attttataca ccttaattta ttgaaaagaa
     3301 taaggtttta tatttcaatt actttaaaat ataaaaatat actaataaaa attgtaaaaa
     3361 tagtataaat ggtataccaa atgtaaattt aatgtgttta gttttatgtc taaaagaata
     3421 cattccaatc atagaaccta tactacctcc aattactgct atagacataa gagtagcttc
     3481 ttttattctc cattcgtttc taattgctct tttcttatct atgtacattg aaaagaaagc
     3541 aatagaatta atcactataa aatatattag aataaaattt ttcacagtct tgcctccata
     3601 aaaatatatt atacattata aaacaagata tttgttttgg aaagttaaat ttgtaattag
     3661 tacatataaa atatctgata agaataaata taatattaat tggaaaattt aaaaaaatta
     3721 aacaattagc aaataggggg tatatttata agacacaaag tgataaaatc tatttagaaa
     3781 ataatgtaaa ataacaggag gataaagcaa aatgaaattt atagtagaaa aagaagtttt
     3841 tgataaatta gaaaatgtat gctttggtgt agtagttgca aaaggaatag ataatactaa
     3901 agaaatagaa agaataagca accttttaga tataagtata gatagagtag aagattactt
     3961 caaagataaa aaagtaaagg aatcagaaga aattatacca tatagagagg catttagaag
     4021 tctaggcatg aacccaaata aatttatgag ttcgatagaa gcaatgacaa caagggttgc
     4081 taagaataag aaacttcctc atataaatcc tattgtagat ttaggaaact ctatttcttt
     4141 aaaatattta cttccaatgg gtgctcatga tatggatttt agaaatgatg atgtatatgt
     4201 tagattttct aaaaaagggg ataaatttgt accatttgga gaaactgatg ttgaattaat
     4261 ggaagaagga gaattaattt attcagttgg agacatggta aaaactagaa gatggatatg
     4321 gagacaagga gaggaaggaa aaattactaa tagctcaaaa aatatatttt ttcctataga
     4381 tggttttact gatgctaatt tagataaggt aatgagtgca agagaagaat tagcaaaatt
     4441 gttaaaggaa atatttaatt gtgaaatcaa agtaggtttt gtagataaag ataatcctga
     4501 aatggaaata taataagttg ttgaatactt gttaagatat tatatttact taatgatgtt
     4561 aaaaaggtat taaaaaagaa aggtcgtagt aaatttgaaa aaaactataa taaagaatgt
     4621 aaatccaatg attgaaaata agtttatagg actttttgaa atagagtata aaaacaaatt
     4681 agatgaagat aaggtttgga tggtagcatc aagaaaaagt agtgagcaat taaaaaatat
     4741 ttatttgaag aatgaagaag atagtgtaga tgcagttgta atagttgggc ttcataaaag
     4801 tagtaaaaaa ttaattttaa taagacaatt tagagtacct attaatggat atatatacga
     4861 gttaccagca ggtcttgttg atgaaggaga gtctatagat atttctgttg aaagagaact
     4921 tagagaagaa acaggactta ctcttttaga aattaaaaaa aataaaagta ttgataaagt
     4981 ttatttatct cctggaatga gtgatgaatc tgttgccttt gtttattgta tatgtgatgg
     5041 tgatataaca gatgaatttt tagaaccaga tgaagagatt gaagcgcttt taatttcgca
     5101 agaagatgca aaagagattt tacaaagtaa tcataagata gacacaaaag catttttgat
     5161 tttacaaatg tttgtgagtt taggagctaa attatttgaa tagatatata gaaaagaggt
     5221 tatatagaag agtaaaataa taaagtttat tttacttgta taaattataa tctctttttt
     5281 tatacaataa agtataattt tgtgtatttt taaataagtt acttgactct taaattttca
     5341 taatatgtta taaaccaatt taagatacta caataattaa aattccaaaa ttatttagca
     5401 tatttgcaat taaaatgaga aaaggtaaag gctgaatgat tgaaaaattt gatgtaaaat
     5461 agtagattag tttaatatta tagtttgtag ggttaaaaat tagaaattgt aaaggagata
     5521 aattctatga aaaaattagt tattatttta attagtatca ttagtgtggt tagcttgaca
     5581 gcttgtaaaa aaagcaatga aagtaaatat aataatttaa acaacaaaga atcaggacaa
     5641 acaaccattg aaatggactt agataaaaat tatgatactt cagacccatt tgttaatgca
     5701 cgtttgtttt gtgtgtctaa tgatatagat gttttagaca cagagatttc ttttaaaatg
     5761 gatggagata gtggtattgt ggaaataaag gacaataaaa cagatgaaac tttatggagt
     5821 aatacatggc atggaagcgt tgataatgat acattcgcaa tttctctttc taatatacaa
     5881 aaagagaaag agtatgctat atgctttacc ggaattaaaa taaatcatgc agttgtaaag
     5941 gttacttttg aaagcaattt agttaaggag aaagaaaaac cctcaaaata aattccagct
     6001 tgtctataac ttgatattca gtacataaaa gcagttgata tttatgattg actgcttttt
     6061 cctatcaaaa aggataggtt gtgcattttt acttgaaata gtttatgcac atcaaaagtt
     6121 ggtgagcatg atattggaaa tggtaagcat gaaatcttga taacacaagg aaataaattt
     6181 aagttttcaa ttaagtatat ttccataatt tagttaacaa taagctaatt attatttata
     6241 ttttgatata taaataataa ttgtggcaaa ttttaaaaaa tcatatattt tttgaactaa
     6301 ataaaaattt gtttttatta ttttaaatta gaggagattc ttgaagatat attattttta
     6361 aaaataagta tagtttaaaa attcaaaaaa aagatggcaa aatatgtccc aagggacatt
     6421 gagtaaaata aatgatttca gcttatttac actaaaaagt acatactaaa tgaggggtga
     6481 aataaagaaa aatgatatac taattgtctt ttgataataa aaaaacatgg aatttgatta
     6541 tttaatcaag aaaaagtatt gaacaaatga tttatttagt gtacaataat catgtaaaaa
     6601 catagtaatg gagggagaca tttcataatg aaaattaagg taggaatgaa cggatttgga
     6661 agaataggaa gagctgtatt aagaatagct caagaagaat taggagataa tatagaaatt
     6721 gtagccataa atgcaagagc aacaacagaa tctttggcac atttatttac atatgattca
     6781 tgctatggaa cttttagagg tgaagtagag gcaaaggatg aagacactct agtagtaaac
     6841 tctaaagaga taaaaatatt aagatatagt gacccagaag aattaccatg gaaagaactg
     6901 ggggttgata tagtaataga atcaacagga ttatttacac aaagagaaaa ggcagaaaaa
     6961 catattaaag caggagcaaa aaaagtaatt ataacagctc caggaaaaaa tgaagatata
     7021 actatagtta tcggagttaa tgagaaagaa tatgataatc aaaagcacaa tataatttca
     7081 aatgcatctt gtactacaaa ttgtctagct ccttttgcta aagtattgga cgaaaaattt
     7141 ggtatagtaa aagggttaat gactacagta cattcatata ctaacgatca aagaatatta
     7201 gataaaagcc ataaagattt gagaagagca agagcagcgg ctgaatctat aatacctacc
     7261 actacaggag cagcaaaagc tgtgtcaaga gtactaccac aattagaagg aaaattaaat
     7321 ggtttttcac ttagagtacc aacaccaaca gtttcacttg ttgatttagt gtgcgaactt
     7381 aaagaaaacg ttacatctga gcaagttaac tctgtgttaa aagatgctgc tgaaggagaa
     7441 ttaaaaggtg ttttaggata ttgtgacaag ccacttgttt ctatagatta taaaggagat
     7501 tcaagatcat ctattataga cgctctatcc acaatggtta tagaagacaa tatggttaag
     7561 gtagtttctt ggtatgataa tgaatgggga tattcttcaa gaactgttga cttagtaaaa
     7621 tatgtagctg aaaaattaaa ataatatata tttcttaaag caaattttaa aaaaattttt
     7681 tgaaaaaatt atataaaact aaaaaaaaag attatgaaaa atattgaagg aaaaattttt
     7741 tcataatctt ttttctttca tgcaaaaaga tacaagttac aacttatatt tgcatattag
     7801 gtcgttagag aagtatatat atatttagta aggaatcata ctttttataa aatgaaagat
     7861 aagatgtcga aaacttgcat aaatctgaaa aatttttatt attaaaatac tgattttcat
     7921 tttaaaaatg ataaaaaaat aacaaataaa gttgcaaata gtaatactta taagtataat
     7981 taaaaaagag aaaaggtttt gttaaacttt ataacaaaaa aagaagggat gattcaaaat
     8041 ggagaagtta acagacagga gacaggttaa ggcagtaagc atggaaacct tcattttcct
     8101 aatattacta gtagttggtt ttgggtatgt tggtagtatt atgggagcag gaatgatgtt
     8161 caaagtaata atgagtacag cacatgcttt actattggag acagttttct taattatggc
     8221 tatggcagtt cttgcaggcg cattaagcgc actactttca gagtttggag taatagcgtt
     8281 aattaataaa attttcgctg tatttatgaa gcctttatat ggactacctg gtgctagtat
     8341 agcaggagct ataacaactt atttatcaga taatccagct attatacctt ttgctaaaga
     8401 taaaacattc acacagtatt ttaaacaata ccaagttcca gcattatgta acttaggaac
     8461 agcctttggt atgggactta tcttaacaac atttatgatt tctcaaggta cagaatatgt
     8521 acttccagca ttaattggta atttaggtgc aattataggt agcgtcataa gtgttagaat
     8581 aatgttgaca tttactaaga agttttataa atacaatccg gaagaagata aagcaacagg
     8641 aacattagag aaaaaggatg aatttagaga aatcagagaa ggaaatgtat ttcaaagagc
     8701 tttagatgca attcttgaag gtggtaaaat gggggtagat atgggaatgg ctataattcc
     8761 tggagtccta gtagtatgta cattggtgat gttattaact tttggaccat ctacagaccc
     8821 tgttacaggt caagaagttt atacaggagc tgcatatgaa ggaataaaat tacttccagt
     8881 tattggagat aaacttggat ttatattaga accattattt ggatttacat caccagaggc
     8941 aatagcattc cctataactt cattgggagc tgttggtgca gcaatgtctc tagttccaga
     9001 gtttataaag tcaggagcta taactcctaa tgatatagca gtatttacag ctatgggtat
     9061 gtgttggagt ggatatttaa gtacacatat tggtatgatg gatgcattaa atgctagaca
     9121 gttggcaggt aaagctattc tttctcatac aataggtggt ttatgtgcag gagcagcagc
     9181 tcactttata tttacactcg taggataaac gtaatcagct aagataaaaa ataatataca
     9241 aaaaaa
//
LOCUS       DACTGW010000155         9215 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.155, whole genome shotgun sequence.
ACCESSION   DACTGW010000155 DACTGW010000000
VERSION     DACTGW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.155"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            400..1056
                     /locus_tag="I9573_002709"
     CDS             400..1056
                     /locus_tag="I9573_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797318.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1526..2797)
                     /gene="sleC"
                     /locus_tag="I9573_002710"
     CDS             complement(1526..2797)
                     /gene="sleC"
                     /locus_tag="I9573_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HAT4797319.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIVAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGADTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            3042..3758
                     /locus_tag="I9573_002711"
     CDS             3042..3758
                     /locus_tag="I9573_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HAT4797320.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            3837..5147
                     /locus_tag="I9573_002712"
     CDS             3837..5147
                     /locus_tag="I9573_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HAT4797321.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            5247..5783
                     /gene="lepB"
                     /locus_tag="I9573_002713"
     CDS             5247..5783
                     /gene="lepB"
                     /locus_tag="I9573_002713"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAT4797322.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            5949..6485
                     /gene="lepB"
                     /locus_tag="I9573_002714"
     CDS             5949..6485
                     /gene="lepB"
                     /locus_tag="I9573_002714"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAT4797323.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6495..7250
                     /locus_tag="I9573_002715"
     CDS             6495..7250
                     /locus_tag="I9573_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HAT4797324.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDIRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVREG"
     gene            7324..8991
                     /locus_tag="I9573_002716"
     CDS             7324..8991
                     /locus_tag="I9573_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HAT4797325.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVILIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
BASE COUNT         3466 a          947 c         1724 g         3078 t
ORIGIN      
        1 tatcagaaaa aatagataat taaagggtta gggtgaaaga gtaatcgtaa aataaatata
       61 tagataatgg tagataagaa tatcagaaaa aatagataat taaagggtta gggtgaaaga
      121 gtaatcgtaa aataaatata tggatgatgg tagataagaa tatcagaaaa aaatagataa
      181 ttaaagggtt agggtgaaag agtaatcgta aaataaatat atagataatg gtggatagag
      241 tagtaagaaa ataaatagat aaagggatag aataaattta aatatggaaa atgaatatct
      301 aataataagt gattaataaa taattgtaga tagattgtaa attaaataaa tctttaatag
      361 aaaggagaac tatttatata ttaaatagta tttataaata tgaaaaaagg cgattttata
      421 tggagtggag ttcttatttg tattattgct gtcttagcaa taccaagtag tagagaagta
      481 tttatggaag caacaggtgc acatccatat attgggggtt ttttaaagtt tgctgtatta
      541 gctacaatgg gagatttact tggtgctaga attttgtata aggattggaa aataccagta
      601 ggagtatttt atagagcaat agtttgggga ataataggtg tagttaccac atatggaatg
      661 tcaatctact catcaggtgt aggtcaagca caagtggatg gtatgcttcc atttgaggga
      721 atagcttttg caaatgctct atttaagagt gcaactatga atatactaat gtcaccaact
      781 gtatttttat tccataaggt tatggactgt tttatagata aaaaatatga ggttggaaag
      841 gggattaagg taaaacttaa agatgtggta aatattgtgg actggaatgc ttaccttggt
      901 tttagtgttt taaaaactat accatttttc tggataccat gtcatacaat tgtattctta
      961 ttccctgctg agtatagagt aattgcttct gcatttgcat ctatagcttt aggtctaata
     1021 ttggctttag ctaataagtc taaagttgca gtttaggttc actgatgaag ataatagtat
     1081 tatttaaatt atgaataact ttttaaatta atattattta tatttataat aaataataat
     1141 ttagataaga aggaatattc taagtgatag atagtgtttt aaataataag gagtaatatt
     1201 ctaagtgaga agtagcattt taaataataa gtgagatttt aaataaaaag taagcttttc
     1261 gttagaatag cattttatta aacttaaaaa taaatataat attttaaaga aatctaagtt
     1321 gctaagcact gtttttaaga agcttaaaat cccccattta aagttttaag attctaaaaa
     1381 aacaagttat aactaaaaaa tggctgttaa gactacaata cttataagta aaaatactta
     1441 tggtatatat tagttcataa cagccatttt tattttaaat taataatttt attcaataat
     1501 ttgagtttat gttctaaatc aagctttaaa ttaaaggatt taaagaagct attctagttg
     1561 tagctacata aactctggat atttcatacc atgttttaaa atctactact ccgctttgag
     1621 gtaaaccaaa gattccttgg aatatcttaa cagcgttttc tgtatctgct ccatagatac
     1681 catcttctat aacttttggt acagctggat aagaattaga tatagcattt aactgatttt
     1741 gtatggttct aacatactgc ccagaagaac caacttgtag tgtagtacca gggaatgaca
     1801 ctggtacacc acttactata ggtgctcttt caaatactat ctcatctcca tagaaatatc
     1861 ttaggatact ctcatagtca tatccttggt cacctaagtc tttactgccc cattgagtca
     1921 tttggtcagg acattgagat ttttgtccat cacaatattg tgctaatagt ggctgtcttg
     1981 atgtaggagg tctttttata aaagtattaa aaatagcatc tacaactacg ttaattggtt
     2041 caaataagtt tctattattt ataaacttat ggtcgtatgc tgttgttgaa gttattgtaa
     2101 agttatatcc tttatttcta taccattcag taaagactct atttaaagta aatgatatta
     2161 tagctattac atttgcatag attgtctgtt caggccaagt tgaatatatt tcagatgacg
     2221 ctatgttttt aatgtactct ttaaatggaa tccaatagtt tggagctgat gaatcttctg
     2281 gcaaaccatc atgaactact ataaattcag gcactacagg gttatctaat acaacgaaac
     2341 cagtaggagg tggtaatggt tttaagttac tttctggaat cttaggagga taagtaccat
     2401 atagagtatg ttctcctatg tcaaaaatca actcactttg tcttgagaaa gaacgcttac
     2461 ttcttgttct aggagacatg ggaactcctt gtcttgctac aattgtagct aacaactgcg
     2521 ttccttctat gacaactgtc tcatatccat ctgctatggc ttccactata tactggctat
     2581 aaggtctaac atcagatggc tgtagagagt aatctacatc tggtgctggt aatactaaac
     2641 cagtcacttg accagattca ttacttctta ggttttggta taaagttgat gagctttggc
     2701 taccattaga cattgaatat atgtttacta ctgcattttg aataggtcta ttgttagtag
     2761 catcaattat gcttactgtt aagaaaccat cttgcatcaa atcacccttt ctttaaatga
     2821 atttagttcc cttatatttc aagatatgaa tatagcttga aatttgttaa ataatataga
     2881 tataaattta aatttttttg agtatttgag tatatttgta tttaaataat ttaaaataat
     2941 ttaatgtata ttttagtatg tatttaaaac acaaataagc taaaaataag tagatgatat
     3001 aaatcataaa taaatcaaaa attttgaaag gaaaataatc tatgaagcta gatttatttt
     3061 tattagcaat agttcctata ttgataggga tgttttggat aaggtctaaa gatagatatt
     3121 gtagagaacc cctaatccat ctaattaaat tttttttaat aggtgctttt ctaagtgtta
     3181 taattatact tttagaaaat ttactgatga agtttaatgt atttgaaggg tattcagaac
     3241 taatatatgt atcatttgta gttgcaggac ttgtggaaga aggtgtaaaa gctctgatac
     3301 tgattcctgc cctaattaaa gaaaaacact ttactgaaaa gttagatggt attatttact
     3361 ctgtattttt agctttagga tttgcaacaa ttgaaaatat ggtttatatt ttttctgaaa
     3421 gtagaaatct agcactgcaa gttggtatta atagggcagt tatctctata ccagctcatg
     3481 taatgtttgc aattactatg ggctactata tttctaagta taaatttgag ggaaataaaa
     3541 ataaaagacg tgagtatttg tttatggcag tgttaattcc aattttatta catggagttt
     3601 ttgatttcat attgatgata gaatatagat gggctattat attacttata gtttatgtaa
     3661 taattttgtg gaagataaat cttgataaac ttgagaaata tatgaatcac tctaaaaaag
     3721 tgtttttcgg taatcttagg aaaaagaaga agaaataaaa tttaaaggaa agacataaat
     3781 aattaaagta taataaatga cacacaagta aagcagattg aagggggatt atagatttga
     3841 cagaattacc aaaggaattt ttagaagata ttgaagaaat actaaaagat gaatatgatg
     3901 attttataaa aagttatgag gaaagtaaga ctactgggct tagaataaat actttaaaaa
     3961 ttgacaagaa aaaattattt gatttggagt tgtttgattt gaatcaaata ccatgggcag
     4021 aagaaggttt ttattatgat gataaaattg acagacctgg tagaaatcca ctgcatgagg
     4081 ctggagcata ctatttgcaa gagccttctg caatgagtgt aataccaaag gcagatatta
     4141 aagagggtga aaaagttctc gatatgtgtg ctgctcctgg tggaaaatct acatatatac
     4201 tatctaagct aaacgataca ggtttattgg tttctaatga aattaatgct agtagaatta
     4261 aggctctagg ggaaaattta gagcgatttg gagcgagaaa ttgtattata acaaacaccg
     4321 attctaatag cttaagaaag acgtttaatg ggtattttga taaggttatt atagatgcac
     4381 cttgttctgg gcaaggtatg tttaggaagg atgaggttgc catatcagat tggagttatg
     4441 caaaggtagt agaatgtcaa tccatccaaa aagagattat cagagatgga tataaaatgt
     4501 taaagaagga tggcatttta atttactcaa cttgtacttt ttcaagggag gaaaatgagc
     4561 atgttataaa tgagtttatc agtgaatatc cacatgcaga acttattgaa atggaaagat
     4621 tatggcctca taagataaag ggagaaggtc attttgttgc caaaataaaa aaattagaag
     4681 aagaagaatg taatgtcaaa gaattaaaaa taaaaaattt agataaagaa ataaaggatt
     4741 atagggagtt tgaaaagaaa tttttaaagg ttgattttta tagtaataaa aaaaataaat
     4801 tttatttaag aggagaaaat ttgtacctac ttcctgaaat atatccagat acgaaaaaac
     4861 taaaagtatt gagatacggt ttgcatttag gggttttaaa gaagaataga tttgagcctt
     4921 ctcatgcact atctcattac ttaaaagttg aagatgtaaa aaatgttgaa aacttcagtg
     4981 tacagagtga atcaattctt aaatatttaa aaggggatgt tgtgaattct aatgatagta
     5041 gaggatgggt tttggtttcg gttgaaggaa tacctttagg ttggggaaaa gaatcttctg
     5101 gtgtaattaa gaatcattat cctaaaggtc ttagaaaagt attttaaact attagaaaaa
     5161 agaagtataa tatttatatt gtaggtttat ggtagattat tttgctgtag attgacttaa
     5221 tatttaacac agaggagacg attttagtgg gtgaagcagt taaaaaagaa gttgtagaat
     5281 ggataaaagt gattgtcata gctcttgttt tggcatttgc aataactcgt tttatagtgc
     5341 caacaatagt caaaggagaa tcaatgtatc ctacattagt tgaacgtgat tatttgatag
     5401 ttaacagaat tgcgtacaag gtaggagagc caaaatacaa agatataata gtatttaaaa
     5461 ccgacttaac agaggaaaat ggaaagaaaa aagatttagt aaaaagagtt atcggggttc
     5521 ctggtgacca tgtaaaaata caagactcca aggtatatgt aaatgataag ttgttagatg
     5581 agacttccta tatacataat aatcgtactg atggagatat tgatatcgta gttccagaag
     5641 gaaaattatt tgcaatggga gataatagag aaaaaagttt agatagtaga tacgatgagg
     5701 ttggattggt cgacgagcat accattttag gaaaggttct agtcagattg tatccatttt
     5761 ctaagatagg aactattgac taaacatata aaatttggag caatcgctga ttagctgaaa
     5821 tcaccagacc atacacaata gttgtctata taaaaaagac aacaccttat ttagtagact
     5881 tatctaaaag atagtgttta agagacatta tcaataaaga taatataact ataaaggaga
     5941 atttgtgaat gaatgaaact attaaagaag agattgtaga gtggataaaa ataattatta
     6001 ctgcactttt ttttgcattt attataactc gttttataaa accaacacta gtaaatggag
     6061 aatcaatgta cccgacactt aaatcacatg attatttggt agcaaatagg atgacatata
     6121 agttatcaga accaaaatgt ggagatataa tgatatttaa gactgattta ttacaagaga
     6181 atggaaggaa aaaagagctt gtaaaaaggg ttataggtgt tcctggtgac catctaaaaa
     6241 ttaaggattc taaggtttat ataaatggta agttattaaa tgaagtttca tatatacatg
     6301 ataattatac tgaaggcgat attgatatgg tgattcctaa gggaaaagta tttgcgatgg
     6361 gagacaatag agaagttagt ttagacagta gatataaaga agtgggattg gtagatgaag
     6421 aaaatattaa aggaaaagtt attttaagag tatttccttt tacagatata ggtatttttg
     6481 agtaggtgat ttgtatggca gttggtatag cgtccttatt gtttaatatt gaggaggctt
     6541 taaacatttg tgagagtata aagcaaataa ctcatttaga gattggaatt gacaatatat
     6601 ctgaatgtag cgagttatgt aaatataaag agagaatttc taagttagga ttatctatag
     6661 ggatacactt gcctatggaa ttaaatactt gcgaaaacat agagtatata cgaaattcat
     6721 ggataaattt tattgagaag atagaatttg aactaaaagg atttgatata aggtatttta
     6781 accttcattt gggatatgtt atgacaaata gagttagaaa gaatagagac aaatatttag
     6841 gaaatagtgt ggatttttta gataaattaa atacaaattc atatgtatgt atagagaata
     6901 cttattcaaa aggtggggat ttttcaaata ttggaaatat atcttatgat tttgaatata
     6961 tatttaagag gattaaaaat tctaagattt gtttttgtta cgatactgga cactgtttaa
     7021 ttgatgagga tgcttatgta aagaacttaa aggataaaat aagactgata cacttaagtg
     7081 ataatgatgg tatcaatgat acacatgttg gtataggaag aggaatttta tctgaagagg
     7141 ggattaaaga ggtcttaaca ttagatgcag aatatttagt attagaaata aattacgaag
     7201 acattgaaga tactataagt aaattgaata atattgtaag agagggttaa atacctctct
     7261 ttatcttgtt gatatgcctc attaactaaa atttatattc atcaaaagga aggagatgtt
     7321 gaagtgaaaa aaataaactt aaaaatagaa ggaaaagata aggaatattc attagaagaa
     7381 aattcccctg gaatcagatt gggagatata gcgaaagaat tttgtgatga gcataagggt
     7441 tatataacac ttgcagttgt agacaataaa ctaaaagagc taaattgtag agttaagaag
     7501 gattgtgaga taaatttttt agacactact aatgaagatg gagagagagt ttactttaga
     7561 gtcatgtcat ttatatttgt aatggcttgt agagaaatct tttgggatag cagagttaca
     7621 atagaacatt cactatcaga tgggctatat tgtgaagttc atatagatag aaagcttaaa
     7681 gaagcagatg ttgaaaatat aaaaaacaag atgaaagaga tagtaaacaa cgattatgtt
     7741 atagaaaaaa ttgaagttac aagaaatgaa gctatttcta tatttgaaaa taatgaaatg
     7801 tatgaaaagg cagagctttt gaaatacaaa gagtatgata gtgctaaagt atataagtgt
     7861 agaaactata tagaccattt ttatggttat atgttgccat caactggtta tataaaatca
     7921 tttgatatta agctttataa tggaggcgtt ataattttag ggcctagtga agaagataag
     7981 actcttccta tgaagtttgt acctcaacct aagctatcaa gtgtatatta tgaagcagaa
     8041 gaatggtcaa gacttatggg aattgacaag gtagtatctc ttaacaaaat aatagaaaac
     8101 aatgaatatg gagatataat aaggacattc gaagcattgc atgaaaagaa gttatcccaa
     8161 atagcggata tgataaagga tggcaataag agagtaattt taatagcagc tccatcttca
     8221 tctggaaaaa caagttttgc tcacagatta tcaatacatc ttagagtaaa taatttaaat
     8281 cctatttcaa tttctttaga tgattatttt atagatagaa aacatactcc tttagatgaa
     8341 tatggaaatt atgattttga atcaatttat gcaatagatt tagagaagtt taacttagac
     8401 ttaaagaaac ttttagctgg tgaagaaatt gatgatataa gattcaattt taaagaaggt
     8461 aagagagaat acactggtaa aaaaataaag atagattcaa atcagccaat tatattagaa
     8521 ggtatacatg ggctaaatcc tattttaaca tcatctatac cagatgaaga taaatttaag
     8581 atatacataa gtgcccttac tcaaataaat ttggatgacc ataatcgaat ccctactgct
     8641 gatttgagaa tgataagaag aattgttaga gactataatt ttagaggata tagtgctgat
     8701 aatactattt tacaatgggc atcagttaga agaggagaaa agaagaatat attcccgtac
     8761 caagaggagg cagatgctat atttaactct gcttgtgtat atgaattagc agttttaaaa
     8821 cgatttgcga aacctttgtt ggaagagata aaggaagata atcctgcaca tattgaagct
     8881 actagattac taaagttttt gcagtatttt gttgaattgg acgatacttc tgacatacca
     8941 ggtatttcaa ttcttaggga atttattgga ggaagtaaaa tagttgatta atatgtgtat
     9001 tatagattgt tttatgaaga ttaaatggca tagcaagtag tgtgattggt ttatgttaag
     9061 tatattaagt gtaaagtgga gaattaaatt gatacagatt ttaaagtttg agttgatata
     9121 taaaaaggct tgttttatac ttttgtatga agcaagcttt ttattttaaa ttttactttt
     9181 ttgtttaata ttttgttagt ttatgtgata atttt
//
LOCUS       DACTGW010000156         9020 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.156, whole genome shotgun sequence.
ACCESSION   DACTGW010000156 DACTGW010000000
VERSION     DACTGW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.156"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            2..1630
                     /gene="aspD"
                     /locus_tag="I9573_002717"
     CDS             2..1630
                     /gene="aspD"
                     /locus_tag="I9573_002717"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HAT4797326.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDEI
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1751..2746
                     /locus_tag="I9573_002718"
     CDS             1751..2746
                     /locus_tag="I9573_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAT4797327.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGIGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(2904..3572)
                     /locus_tag="I9573_002719"
     CDS             complement(2904..3572)
                     /locus_tag="I9573_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAT4797328.1"
                     /translation="METVSNNQSSVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(3858..3983)
                     /locus_tag="I9573_002720"
     CDS             complement(3858..3983)
                     /locus_tag="I9573_002720"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797329.1"
                     /translation="MGVSTSQLIKIITRKTRMVSSSILMGVADISKLGLFEDVIK"
     gene            complement(4410..6830)
                     /locus_tag="I9573_002721"
     CDS             complement(4410..6830)
                     /locus_tag="I9573_002721"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAT4797330.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(7184..7534)
                     /gene="rsfS"
                     /locus_tag="I9573_002722"
     CDS             complement(7184..7534)
                     /gene="rsfS"
                     /locus_tag="I9573_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAT4797331.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(7651..8223)
                     /locus_tag="I9573_002723"
     CDS             complement(7651..8223)
                     /locus_tag="I9573_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAT4797332.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSRNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(8224..8913)
                     /locus_tag="I9573_002724"
     CDS             complement(8224..8913)
                     /locus_tag="I9573_002724"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAT4797333.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         2961 a         1531 c         1203 g         3325 t
ORIGIN      
        1 catgaaaaat catacttata aagaaatcaa aaacatatat ggaaaaataa gcccttttga
       61 atttaaggac aaattaatag atattgcaaa atacactgca aaagaaaata acagagaatt
      121 acttgatgca ggtcgtggaa atccaaactg gacttgttca actgctagag aagccttttt
      181 tacttttggt catttcgcta ttactgaaac tcgttctaat tgggatttag gtcatttggc
      241 tggaatgcct caaaaaaagg gtattaaaga aagatttttt aaatttataa atgaaaatat
      301 agatatgcct ggagcatatt tagctagaga tattataaac tttggtataa atgaacttgg
      361 atttgatgga gatgaatttg ttcatgaact agctgatgga attattggtg ataactatcc
      421 acttccagat agaatgttac ctcatatgga aaaaatagta cacgattacc tagttcaaga
      481 aatgaaatat gatatatcag gtaaatatgg tgatgtagaa atttttgctg ttgaaggtgc
      541 tacagctgct atgtgctata tatttgattc attaatggca aatgaacttc taaaaaaagg
      601 agatacaata gccttaatga ctccaatatt tacaccttat cttgaaatac ctaaccttcc
      661 tcgttacgac tttaaagtgg taaatataaa tgctaatgaa gttgatgaaa aaggtgctca
      721 tacatggcaa tatacaaaaa aagaattaga gaaacttcgt gacaaatcta ttaaagcttt
      781 attcgttgtt aatcctaata atcctgcttc tatagctatg gatgaaatat cttgtaataa
      841 tctaatagat gtaattgaaa attataataa agatttaatg ataatatcag atgatgtata
      901 tggaactttt gtagaagatt tttcatcctt aatgtcaaaa ctaccttaca acactgttgg
      961 agtatattct tattcaaaat attttggagt tacaggatgg agacttggta catttgccct
     1021 tcataagaaa aatgtttttg ataagaaaat aaatgattta actggagaat taaaaaaatc
     1081 agtagataag cgttacagtg atatgagtct taacccttct agtctttcat ttatggaaag
     1141 agttgttgct gatagcagac ttgttgctct caaccatact gctggtcttt ctactccaca
     1201 gcaagtacaa atggcgtttt tctctgcttt tgcattgatt gacaaagtag atgcctacaa
     1261 aaaactcaat atgagtatct gccatagacg tcaaaaacta ttatttgaag ctttagagct
     1321 tccaattaat gaaaacaaaa acaatgctgc atattacact caatttgata ttgaagaatg
     1381 ggctaaatta aactacggag aagacttatt taaatttata agtaaaactg catctccagt
     1441 agatgttctt tataaattag ctaatgacta ttcagtagtt ttacttagtg gaaatggatt
     1501 ctatggccct gaatggtcaa ttagaatatc tctagcaaat ctatatgatg aagcttatac
     1561 taagatagga aaagccataa gagagatatt aaatggttat atcgttgaat ggcaaaaaat
     1621 taagaaataa ttatattaca ctgaaatatc taaattatat gaataaaaga gtttatgtag
     1681 accttaacta ctttttttga tataatccta tcggaattat aaattttatt tatgaaaggc
     1741 agagaatcgt atgagtatta agaaaaaagt tgccatagtt tttactggtg gaactatatc
     1801 tatgacagta gatgataaag tcggagcagc tataccaact ttatcaggtg aacaaatact
     1861 atcaatggtt accaacattg ataaagttgc agatgttgaa gtttttaatt ttgatgaaat
     1921 tccaggacct cacataacac cttatagaat gatggaattg aaaaattatg taaatgattt
     1981 attagcaaga aaagatatta caggtgttgt aataacacat ggtacagata gtttagagga
     2041 aacagcttat tttctagacc ttacaattga caacatcaaa cctgtaatag tcacaggtgc
     2101 tatgagaagt agttctgaac ttggatatga tggttcaagt aatttatctg catcagtttg
     2161 tactgcagta tccaaagatg ctatgggaaa aggagtcctt gtagttttaa acaatgaagt
     2221 attattagct tctgaagcta ctaaaacaaa tactctttct ctaaatacat ttaaatctct
     2281 aactagtggt ccattaggaa taattgactg caacgaactt gtccttacaa gagatattgt
     2341 aaatagaaca attatagata ctgataaggt agaatctaaa gttgccttat ttaaagcata
     2401 tgtaggagaa aatgctgact ttataagatt tgctgttgat agtggttata aaggtatagt
     2461 tattgaggca atgggtatag gtaatattcc tcctcaaatg ctttctggag ttgaatatgc
     2521 tagagaaaaa ggacttcctg tagttatagt ttctagatgt cattcaggta gagtattcga
     2581 tagttatggt tactttggtt ctggtagaga tttaaaaaat cttggttgta tatttggtgg
     2641 ggacttgcca ggtcaaaaag ctagaataaa acttatctta gcacttggaa aaactgataa
     2701 ccttgatgaa atcaaagatt tctttgaaaa aggaatctat tgctaattct aataacttct
     2761 atttaattat ttagttcagt ttacaagaaa atacacacaa accataaaat gagatgttga
     2821 caacataaca gacagtatgc tcctgtaaaa ggtaacatac tatctgttat attgtctgga
     2881 ttcccaaatt agctatttgg aacttaacta aaaaatccta atataagggc aactattata
     2941 atggcaatcg gaatacatac tgtgtaatcc tgttccatag agtcatcatc tgaatgtagt
     3001 tttacaattc gcaatttagc aatcagtttg aagcagaaat agcctattat tgttccaatt
     3061 acattcgtga gtaagtcgtc tatatcggtt gctctgtgta acgtaaaaag ctgaatagtt
     3121 tcgataaaga gagataatcc aaaaccaatc aagaatgtat ttctcaaccg ttcaaaggtt
     3181 ttgctgataa gcggacataa aaaacctaat ggaacaaaaa gtaaaatatt caaaataaaa
     3241 ctcaaactaa atccatcact aaaaggaata agatttatgt ttggattaaa gaacgtttcc
     3301 cctaactgtg acagtcttat atattcactg aatgtaggga taccaacaat atgcgatagt
     3361 gttaaacata agtagtaata cattgccagt gataaaatag atgtttttac ctttggaata
     3421 tcactatttt tcttgaatat tacaactaag aaaataccaa tcaagataat tcccaacaag
     3481 aaaataggat taattaacaa taaatctttg atagcttcaa acagactgag ttgaatttct
     3541 acacttgatt gattatttga tacagtctcc atttgtacac accccctcat aacatttcaa
     3601 ttatttctaa aaaacaaaat agaagataaa tcttaaaatc atcctatcaa aatttttaaa
     3661 tttgccttta tcagtttata ccaccagtta attcctgtct tacttctgta tggtattctt
     3721 gggattcttt catcaaatta gacaaaaaaa tggttaccat atcaataaac tggttacgct
     3781 ccatatctcc tgaagcaaac atcccctgag aataaatccg tatcaggaat ctccacatct
     3841 actgcaccta tcacaagtca tttgataaca tcctcaaaca atcccaactt ggatatatca
     3901 gcaacaccca ttagaatact actgctgacc atccttgttt tcctggtgat aattttaatc
     3961 aactgggaag ttgaaacgcc tatctttttt gcaagttcct ttttagaaac atggtagtta
     4021 ttatatgaat ctgaatctcg ttgtctgggt gtttaagata aagcatagat acgcaccttt
     4081 tttgcctgtt ttcattatat aaaaaaaatt ttaaaaatac aataattata tttttttcgt
     4141 ttaagcttag agcagttcct tgatgaacaa cactttagaa actgaataat ttacatacca
     4201 cttaatacat taacacgatt taacattaat tataataata tttaaaacaa caattagagt
     4261 gtataagaat gaacttttta gttctttaat catttttttg tataaaatca aatctatttt
     4321 tgtaatttca acaatgaaaa aaggcttatc tctattttga gacagcctca tttattttat
     4381 aaatttatat ttttgttcat atttttgagt tatttaacaa ctatatttac taatttttta
     4441 ggtacagcta caactttaac tatagtttta ccttcaacta atccttttat tttttcatct
     4501 tctaaagcta ccttttccat ctcatcttta gatatatttg agttaacagt taattttcct
     4561 ctaacttttc cattaacttg tacaacaact tctatttcat cttttactaa agctttttcg
     4621 tcatatttag gccagtctat atcaaataca cttccttctt tacctatcat agtccatagt
     4681 tcttcaccta aatgaggggc aaatggtgca attatagtta ctatagtttg tacaccttct
     4741 tttataactg cttcgtttat attatctaac tctttatatt tatacatatc atttattaat
     4801 tccataagag ctgatatagc agtattaaat ccgaattttt cacttaaatc agcagtaact
     4861 tttttaagtg tagagtgtat agtatatctc atatccttat cttgggaatt taattctcca
     4921 aactctacat cctttttaac cacatctgct aactcgtcaa ccaatctata aaccctattt
     4981 aagaatctaa agcatccatc aacaccttgc tctgaccagt ctaaatctct ttctggtggt
     5041 gctgcaaata atacgaaaag tcttgcagta tctgctccat actcatctat tatatccata
     5101 ggagatactg tattcccttt agacttactc attttagaac catccataag taccattcct
     5161 tgagtaagta agtttttaaa tggctcattg aagtctacca ttcccatact cttaaatgcc
     5221 tttacaaacc atcttgcata aagcaaatgc attatagcat gttctactcc acctatatat
     5281 tggtctactg gatgccatct attaactaat tccttactaa atggttcatt ttcattattg
     5341 ctatccacat atcttaagaa ataccaagaa gaatcaacga atgtatccat agtatcaact
     5401 tcccttctag ctggttttcc acagtgagga caagttgttg acataaattg ctttgaagtc
     5461 gtaagtggag attctccctt tccagtaaat tcaacatctg ttggtaaaag aactggtaaa
     5521 tcttcctttt taactggaac tattccacat tcatcacaat aaaccacagg tataggacaa
     5581 ccccagtatc tttgtcttga aactaaccag tctctaagtc tataattaat tgttctcttt
     5641 cctctacctt ctttttctaa cttatcgatt attccttcaa atccttctga agcatctaat
     5701 ccattaaatt cttctgaatt tatcattttg ttatcttctg ttattacatc aattatgtct
     5761 aaattatatt tttcagcaaa atctctatct ctttcatcat gtgctggaac tgccattata
     5821 gcacccgttc catactcaac taatacatag ttagctatcc atagaggaac tttcttgcca
     5881 tttaaaggat ttataacata tctacctata aacattcctt ctttttctac atcagctgct
     5941 gttctttcta tttctgtcat agtatgcatt ttttgaacaa aagcttcaac atctgcttca
     6001 tattcagtcc ctgcaaccaa ttctttaact aattcatgct ctggagcaag taccatataa
     6061 gtaactccat aagttgtgtc tggtctagtt gtaaatactg tcatagattt atcagttcca
     6121 tctatatcaa aaactaaatc tgctcctgta cttttaccta tccaattttt ttgcattgtt
     6181 ttaacttttt ctggccatcc atctaatgta tctaagtcat taagtaattc ttctgcaaat
     6241 tcagtagtct taaaatacca ttgttctaaa tctttcttta atactgttgc tttacatctc
     6301 tcacatgctc cttgaacaac ttgctcattt gcaagaacag tctcacaaga aggacaccag
     6361 ttaacatatg attttttctt atatgcaagc ccatgctcta aaaacttcaa aaatatttct
     6421 tgtgtaaatc tatagtattc tggagttgaa gttgcaactt ctttatccca atcataacta
     6481 atacctaata aatttaattg ctctttcatt tcttctatat tttccattgt ccatttatgc
     6541 ggatgtattc catgttttat agctgcattt tcagctggta atccaaaaga atcccatccc
     6601 attggatgaa gtacattata tccttccatt tttttgaatc ttgcgacaac atcacctata
     6661 gaataatttc taacatgtcc catgtgtatt tttccagatg gataagggaa catttctaat
     6721 gtgtaataat taggtttttc agtttgtgca gtatccattt tatactgatt attatctttc
     6781 caaatttttt gccatttcga ttctacttct ttaaaattat atacgctcat attttgcctc
     6841 ctatttttaa ttgtgtataa ctaccttatt tagtattatt tacttataat ttccaaataa
     6901 ttctttgaaa ataaaaaatc ctcgtcacta aacttacgtt tagggacgag gataaatctt
     6961 cgcggtacca ccctaattag cctactacat atatagactc tcttagatag cttttaatcc
     7021 agccatggag aatagatttt aatctagtaa gttcaaagtt atataatatt agttctcacc
     7081 ttccactaac tctctgaaaa aatataatct tttactgctc tagtctattc aatttaatat
     7141 aatttttgaa attttctaat tataataaca tatattatca tgtttatgct aatgtgtcaa
     7201 tatctatata aggtgcatct ttccataatt tttctaaatt ataaaaccct ctattttcct
     7261 cattaaatac atgaaccatt atgtctccat aatcaaggag tacccaagtt tgagttcctt
     7321 gtccttcttt acctcttggc tctaatccta gttttgctaa ttcatcttcc acattgtcag
     7381 caatagcttt tacctgtctc tgagaagatg ctgtagtaat aataaaataa tcacacaaac
     7441 tagaaacttt tcctatattt attataactg tatcttgacc cagtttatct tctatggcat
     7501 catatgctat ttttgtcatt tgttccactg tcatataaaa attcctcact ttcctaatta
     7561 aaatacactt agttattgta acatattaat acgattttgt aacatagtca aatttaaatt
     7621 tattaatatg tttgccttta tatagctttt ttataataac ttttctttaa ttagatagtt
     7681 tcttgccata actgttcttg gatgtatctc ttctttttta ttgatgacaa acattaaagt
     7741 attattaaaa gaagttaaaa gtgcttcatc aagcttccct ccatatgcta attctcttaa
     7801 tgtatctaca actggaaatt ttcttccttc ttcaattaaa tcagctatgt aaattatttt
     7861 ttctaaaaga gacatgtcct ctctccctgt tgtatgatat ttgattgcat ttataatatc
     7921 ttcatcttct acttcaaatt catactcgca tatgtacgcc ccaattacac tatgagacaa
     7981 ggaacgattt ccatcctcta ttgggtctaa atatatttca tacttattta cataatcctc
     8041 tatttccttg tcacttaaat actttgcaca atcatgaatc attcctgcta agtaagcttt
     8101 ttctttgtca catccataaa tctcacataa tttttcagca cattttgcaa catttcttga
     8161 atggctaagc ctccccactg gtagcatttg atttagtctt tggttaatat tttctaagtt
     8221 catttaatca tctccatatt gatatagttt attttcatat aaataatttt ctacattttc
     8281 gggtactaag taacgtattg acttgccttc atttagctgt tcccttatat atgtggacga
     8341 tatatccagt gatggaacat aaactgttat aatgtcagca ttatacttct tagttagttt
     8401 ttctatagtc tcttgtgacc ttaacaaact aattcctggt ctagttgcgg ctataaaagt
     8461 agctaactca aagtttttct ttacatcttt ccactcttca acactacaaa ttgcatctgc
     8521 acctgttata aaataaatgt ctgcattttt gtattttttc tttaagtatt ttaatgtatc
     8581 tattgtataa gtccttttct ttctttcgat ttctactttt gaaactaaaa aatcttcgtt
     8641 gctttcagtt gctagaagtg tcatattata tctatcatac ttatctgtag tgatacataa
     8701 tttatgtggt ggatttcctg atggtataaa gattattttg tcaatgtcat acttgtctct
     8761 aataaattct gctgttgcta aatgagcata gtgtataggg tcaaaagtac ctcccaatat
     8821 acctattttg attttgcctt tatggcgatt aaatttctct agtttttcag tatttttcaa
     8881 ctctgccatc ttaactagat tttcacttct catattttgt ctccttttaa tgttataata
     8941 ctattgtacc attatttttt atttcttaaa gcttataatt tgtttttttg ggtatataat
     9001 tatatttata tgctaaaaaa
//
LOCUS       DACTGW010000157         8963 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.157, whole genome shotgun sequence.
ACCESSION   DACTGW010000157 DACTGW010000000
VERSION     DACTGW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.157"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            56..904
                     /locus_tag="I9573_002725"
     CDS             56..904
                     /locus_tag="I9573_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAT4797334.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            983..2905
                     /locus_tag="I9573_002726"
     CDS             983..2905
                     /locus_tag="I9573_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT4797335.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDIHDSSVIAAYLEGCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            2987..3850
                     /gene="aroE"
                     /locus_tag="I9573_002727"
     CDS             2987..3850
                     /gene="aroE"
                     /locus_tag="I9573_002727"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAT4797336.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGIEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNAKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4050..5267
                     /locus_tag="I9573_002728"
     CDS             4050..5267
                     /locus_tag="I9573_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4797337.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLIVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5503..7368
                     /locus_tag="I9573_002729"
     CDS             5503..7368
                     /locus_tag="I9573_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HAT4797338.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIEYNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7546..8910)
                     /locus_tag="I9573_002730"
     CDS             complement(7546..8910)
                     /locus_tag="I9573_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4797339.1"
                     /translation="MSQLLIKNAYLITMNADREVYETGSILIENNIIKNVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLIRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHNPGAAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
BASE COUNT         3321 a         1070 c         1588 g         2984 t
ORIGIN      
        1 ataaaatcaa agatactatt aattataaag aaaataaagg gggcaaattt aagctatgaa
       61 taatcaaaaa aatcttaata ttccaattac aattagttct tatacattgg gaacagaagt
      121 ttcattccgt gatagagttc gtattgcaaa agaagctgga tttgatggta ttggattgcg
      181 tgctgaaaat tatattgatg caaaaaatgc aggtgttaca gatgaagaaa tgattgcaat
      241 tcttaatgaa tatgatatga aagttacaga agtagaatat ataactcaat ggggaacaga
      301 agaagataga actgaagctc aaagagaaaa agagcaaaat gtttatcata tggctcactt
      361 atttaatgtt aaacatataa attgtggatt attagagatg ataccagaag aacaaattat
      421 aactgcattg ggagaattat gtgacagagc aggtgaactt attataggtc ttgagtttat
      481 gccatatagt ggtgtaccag atttggctac tgcatggcgt gttgtgaaag gatgtaatcg
      541 tgaaaatgca atgcttatat tagatacttg gcactgggct agagcaaagc agacagcaga
      601 ggaccttgca cctgttccag cagataagat agtatcaatt caagtgtgtg atgttttaga
      661 aactccttat gaaaaactta gggatgaatc actacatgac cgtttggctc caggtgaagg
      721 atatggaaac acagaggaat ttgtgaaaat aataaaagaa cacaatatat caccaagagt
      781 cataggggca gaagtaatat ctgattctct aatatctaaa gggttatcca ctgctgctga
      841 aactgtattt aatgcaacta aaaaagtttt ggataaagca tggtctgaag tatctccaaa
      901 ataaacttat ataatataaa aatatacaga gtattataat ataaaacttt taaatataaa
      961 acttgaaagg aaggaaaacc ttatgaagat tagttctatg tttacaccta taagaattgg
     1021 ttcaatgaca gttccaaatc gttttgtagt tccccctatg ggaaacaatt ttgcaaacac
     1081 agacggtact ttaagtgaaa cttctaaggc ttattatctt gagcgtgctt taggtggttt
     1141 tggattaata actatagaat ctagtgttgt agacaaaaaa gcaaaaggag gtcctcgtaa
     1201 gccttgttta tatgatgata gcacaataga tagttttaaa aatgtaatag atgcttgtca
     1261 tgatgcaggt tcgaaggtat ctatacaatt acaacatgct ggttctgaag gaaatgaaaa
     1321 agtagcagga catccattaa aagctgcttc tgcaatacct gcatcaaatg gtcgtaatac
     1381 accactagca ataacaacag aagaaatata tgaacttata gaatcttatg gagatgctgc
     1441 attacgtgca caaaaagctg gtgcagatgc tgttgaagtt cactgcgctc atggatatct
     1501 ggttagtagt tttatatcac aaagaacaaa taagcgtgta gatgagtttg gtggatgttt
     1561 tgaaaataga atgagattac ctcgtcttat cattgaaaat atacgtaaaa aagtaggaaa
     1621 ttctctagca atattatgtc gtataaacag cactgatgat gttccaggag gaattgatat
     1681 acatgatagt tcagtaatag cagcatatct agaaggttgt ggaatagatg gacttcatgt
     1741 ttcacgtagt atacatatac atgatgaata tatgtgggct cctacgactt tacatgctgg
     1801 attcagtgca gaattagtta ctgaaataaa aaaagctgtt agtataccag taataacagt
     1861 tggacgttat acagagccac agtttgctga gttaatggtc agacaaggaa gatgtgattt
     1921 agtagcattc ggtagacaaa gcttggctga cccagaaatg cctaataaag ctaaaaatgg
     1981 aaaactagac gaaatgatac catgtattgc atgcttacaa ggatgtgtac caaatatgtt
     2041 ccaaggaaaa cctatagcct gtcttgcaaa tcctatatta ggacatgaag cagagcttaa
     2101 gccagctgaa attagcaaag aagttcttgt tgttggtgga ggtgttggag gtatgcttgc
     2161 agcatgggta tgtgctaaaa gaggacataa tgtaacttta gttgaaaaat ctgaagttct
     2221 tggtgggcaa atgcgtttag ctgcatatcc tccagggaaa ggagatatta ctaacttagt
     2281 tagaagttat atttcaaaat gtaatcaata tggtgtaaaa atatgtacta atacagaagc
     2341 tacagtagaa cttataaagg agaaatcacc tgatgtagtt attatagcta ctggagcaac
     2401 tccacttgta cttcctatac caggaatcaa tgattcagga ttaattcatg ctgtagacct
     2461 tcttgatggt aaaaaatctt gtgggaaaaa agtacttgtt gttggaggtg gaatggttgg
     2521 atgtgaagtg gcagccttcc ttggagaaca agaacatgag gttactgtta ttgagcttag
     2581 agaagaagtt ggtgcagatg taatttcaga acaccgtaaa ttccttatga atgattttga
     2641 aaattataat atacatacca ttactggtgc aaaagtttct aaattttatg atgatggtgt
     2701 cgactattta cttacagatg gcacagagca tgatttaaaa ggttttgatt cagtagtact
     2761 tgctatgggt tctcgtaatt acgacccatt aagtgaagtt ataaaagaag ttgtaaaaga
     2821 aacttatatt gttggtgatg cagtaaaagc tcgtagggca ttagatgcaa caaaagaagc
     2881 ttttgaagta gcattaaata tataaataat aaataaattt ttctttgtaa ataactgaat
     2941 aggttatttg caaagaaaat gataatattg ggaggaaatt atagttatgg aaatttcagg
     3001 tagaacagga ttatttgcat taataggtac accagttggt cactctaaat caccagtgat
     3061 gtataattat agttttaaga aattagattt agattataga tatttagcct ttgatatcac
     3121 agtagataag gtaaaagagg cacttttagc aataaaaact tttaatataa aaggagccaa
     3181 tgttactatg ccatgtaaat ctgctgttac agaatatatg gatgaacttt ctccagcagc
     3241 tcgtataatt ggagcatgta atacaattgt aaatgacaat ggaaagttag ttggtcatat
     3301 aacagatgga gtaggctatg ttcgtaattt aaaagaaaat gggatagaag ttaaaggtaa
     3361 aaagattaca attatgggtg ctggtggtgc agcaactgct atacaagtac aatgtgcttt
     3421 agatggagca agagaaatat ctatctttaa tgctaaggat gatttttata aaagagcaga
     3481 gcagacagtt gagaatataa aaaaagatgt accagaatgt gttgttaatc tatatgattt
     3541 agaagataca aacaaacttt atgaggagat agaatcaagt gatatattga caaatgcaac
     3601 tcttattggt atgaagcctt atgacaatga aacaaatata aaagatacta gtgttttaag
     3661 aaaagactta gtagtaacag atgtcgttta taatcctaag aaaacaaaaa tgattgaaga
     3721 tgctgaagct aatggatgta aggctattgg tggattagga atgttattat accaaggagc
     3781 agaagctttt aatctatata ctggattaga aatgcctgta gaagaagtta atgaattatg
     3841 ctttaaatag ataagagtga tatcttaaac aatgaaaaaa catacgtatt tcaatatttt
     3901 taataataaa ttacaaattg tcatagtggg ttttgcatca tagtccagtt aaatttgaag
     3961 ctttaactga ctatacttta aatccattat gaggatgagt ttattgtaca caaaaatgtt
     4021 aataaattaa caagaatggg agaaaaatta tgaacaataa aaaatattat cctactgcca
     4081 tagcgttgta tttttcatat tttttacttg gtattggaat ttcaatttta ggacaataca
     4141 aaccagaatt ttcatcaatg tggggggcta aaacactatc agatggaaca ttggatgtaa
     4201 gtattgtttt agctgttatt gctgcactag gattagggag acttatatct tatccatttg
     4261 caggacctat ttcagataag tatggaagaa aagtaagtgg tcttatagga aactttctac
     4321 atgcaatttt ctttgttgga attgtatttt caccaaattt ttatatagca tatgtatttg
     4381 caataattgg aggggcagca aattcattct tagatacctg tgtaactcct tcttgtatgg
     4441 agatatttgc tagtttagga acaatagcaa atatgtttac taaatttaca atagctttag
     4501 cacaattttt gcttcctttt ataattggat ttgttgcagc aaattcaata tcatttaaag
     4561 ttatttttat agtaacagcg attcttattg tagtagatgc cattttgata gctgtgttac
     4621 catttccacc agctaataat gttattgata acaaaggcaa aacagttaaa tcagagaaga
     4681 tgaaatttac accaacaagt atagctcttg tttgtattgg atttacatgt acatctacat
     4741 ttgtattatg gttaaattgt aatcaggaat tgggtaaatt gtatgggatg gctgacccta
     4801 caaaaattca atccttttac tcaatgggag taatatgtgc agtattaata acttctttac
     4861 ttattaaaaa atatattaaa ccaataagaa tattagttat atatcctata atagctttac
     4921 ttatgctatt aattgtatat tttgttcaaa ctccaactat ttgtatgtta gggggatttg
     4981 taataggtta ttcagcagca ggaggtgttc ttcagttaac aacgtcaact gcaaatgaaa
     5041 tgtttccaac taataaagga aaaataacat ctatagttat gattgcatct agtatagcaa
     5101 actatgttat tttaaatata gcaggtatta ttacaaaatc agggggagtt aatggaccaa
     5161 aatatgttgt attgtttaat gtggcaataa catttgttgg aattttactt gcattgtttg
     5221 taaacatgag atatgaaaaa gaaaaagtgt atgattatga tgtctaatat tagtataata
     5281 tatgcataga gaagtctaaa tctaggatag atttttatta aaagtaatta aaattaataa
     5341 ggatattatt tacagttata agatagggta tttacataat taataataag gatattacta
     5401 gaatttcaat ttctagtaat atttttttgc gtataaatgt aaacttgacg aaataagtag
     5461 tataatcaat ataaattaca tcatgtaaag gaggataatt tggtggagaa tgtttttaag
     5521 cgattgcagg aatttaatgg ttatgatgga tacaaagaat catttgaaat gaattaccta
     5581 tgtatatatg aaagtatacc gttaagagaa caagtagaac ttgcaaataa tctagtagat
     5641 gaaattttaa atatgtataa aagtgaatct aatgaaattt atttattgga ggatagtaat
     5701 tctaagtctt taatttgtta tttcgaaatt tttatgaaaa agataaatac tttggtaaaa
     5761 gaaatgatta ttgatgagaa atggttatat aaattaacta aagaattaat atataagagt
     5821 aaaaaagttg agtatgtaaa actagggtta gttttaagtg agaaatatct aaatgtagaa
     5881 aacttaagag aagttgtaga tacattttct aaatctggag aatacgtatt ttatctgagt
     5941 aatactatta aaaaacttga attttacaat acatatttat ttaatttatc taaaaaggct
     6001 acaggttcaa taaaagtatt tgctattgtt aatatggaaa atttagattc taaaataaat
     6061 tcctatctaa ttgaagatgg ttataaggat acaaagtacg aacgattatt gatgaactat
     6121 attatttcaa ttgtagattt gaatgaatat cttgaaaaac gagatttaga taaagaaaag
     6181 ataaataatt tagcacgtct tatatgcaat tacttgctaa gtgtagaatt taaatatata
     6241 ggtaataaat tagaattagt gaatcgattt ttacctactg tagtaaatta tgggactaac
     6301 tttgaaagtt tatattcaat tttcctaata gctataaacg tcttaaaaga tgaaaatata
     6361 gagtacaata agattgaatt tgaaaaagaa ataaatgata tcttgctcag tgaaaaatgg
     6421 aaaaatatat attttgaagc attaagagat gctagtggaa aaacagaaga tattataaaa
     6481 atgtcagaaa tttatgatgt aaacttatct tttgatgact tgttgccata tctgaataga
     6541 gatattagag attttgaggt atattggcat atatctaaaa agggaacgac aagttctaga
     6601 ttaaaattat taaatttttt tgaagagacg tttaaaatag atgatttaat tggaaaaatg
     6661 aaagatatag aaaaagataa attgactcaa gaatattatg atgatatgtt attttttatt
     6721 gtgcttaaag gaagtaaatc actatatcct gaaggaaaga atatatcttt aaaaggaata
     6781 tttggaaata taaatgaagt aagaaaagaa tccataaata tcttaaaaag atacagagag
     6841 aaactatctc ttgaagaatt aaaaattgtt aaagaagcgt atgaaaaaga aaaaaatgtt
     6901 atattgaagg atgaattaag aagagtatta tatgaaagta ataatttaaa aaaagaattt
     6961 gtaaatattg aaaaaataaa agtagatgaa catggtaagg atatctattt gacttcaata
     7021 gctgttgctg gttcaaggtt tagaaataga gaatatcttg aaaaagaatt ggagaagagt
     7081 aagatatact atttaactag agagaaagat aatctatatg atgaaaaagc tattaaaata
     7141 gttggtgaaa ctggatatgt aataggctat gtcccaagaa aagaaaacta tattttgagc
     7201 aatttattag atggaggaaa actgctgtat tgtagagtaa ctgaatataa tttatatgaa
     7261 gattgtatct acgcaaatgt gtacttgagt tataaagatg taattgaaac agttgaaaac
     7321 tctttaaaga tggttttaga taagagtaga ataaaactta taaactaaga cgtttatata
     7381 ttgcttagat gatgtaatcc taactgattg gttatttttt ctggaattat ggtgtatttt
     7441 atgagcaatg ttaatgaata taaaataaaa aaacaattgt tttgtatata tgatgtgtat
     7501 aaatatattt atatattcat atttacaaaa caattgaaat ttatgttatt ttatgatttt
     7561 aaatttagat ggaattttaa cttctgcttt atcttttatt ttagaagctt gttttttaac
     7621 tttatttaat aattcagatt catctacaac taatactttt ctttccttca tcaaccattt
     7681 accatcacaa atagtagaat ctacattttc actagtcata gaataaacta tatttccaat
     7741 tggattatgc attggtaaac aatgaattgt atctggattt agaataatca tatcagcttt
     7801 ttttcctact tcaagactac caatttcttt ttctagtaaa gcacattttg ctccatttat
     7861 agtagccatt tctaagactt cttcagctgg tacgacagtt gggtttaaag ttctaccttt
     7921 atgtattagt gatgtaagat acatatccct cataaggtcc attctattat ttgaaggagc
     7981 tccatcagta ccaattgaaa caggtattcc tttttctaac atttcaggaa ttgaggcaaa
     8041 tcctagaact actttcatag ctgcacctgg attatgagaa acttttacat catgtaatct
     8101 aaataaatca atttctttat gagtaagcca aactgtgtga gcagctaaaa gatttggtcc
     8161 taatacacct agtttatcaa gatattcaac tgttccaact ccgttgtttt gctttacaaa
     8221 tgaaatttca tcagctattt cagcaatatg catatgtata cctgtattgt atttatcagc
     8281 taactcttta gtcctaataa gcaattcttc agttgtatta aaaatagttc ttaatgcaaa
     8341 ccatatttta atcctctcat tagatgtgtt attaaactta tggaataagt ctaattgagt
     8401 gttgatttcc tcatcagtag ttttttgcca tgcttcagga agaccatctc ctgtatccat
     8461 aactgattta gctaaacagg ctctaagtcc agttttatca acagcttcaa ccatagcatc
     8521 tacatattga ccaccagatt ctaaaaatgt agttacccca gattttatca attcaacaca
     8581 acatgcagta gatgaaatca atgaatcttc atagttgaag ctactttcgt atggccagat
     8641 tctctctcta agccaagtta aaagtacaac atcatctgca agaccacgtc ctaattgttg
     8701 ggataagtga acgtgtgtat tgactaaccc tggcattagt atttttcctt tagcatcata
     8761 tatctcagca tctttatcta caatattaat atcaaactta ccaacatttt ttataatatt
     8821 attttcaata agtatacttc cagtttcata tacctctcta tctgcattca tagtaattag
     8881 atatgcattt tttataagta attgtgacat aagtacaacc tcttttctat atatttttat
     8941 atctatgtat tatgataata aat
//
LOCUS       DACTGW010000158         8897 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.158, whole genome shotgun sequence.
ACCESSION   DACTGW010000158 DACTGW010000000
VERSION     DACTGW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.158"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(226..741)
                     /locus_tag="I9573_002731"
     CDS             complement(226..741)
                     /locus_tag="I9573_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HAT4797340.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(1104..2174)
                     /gene="leuB"
                     /locus_tag="I9573_002732"
     CDS             complement(1104..2174)
                     /gene="leuB"
                     /locus_tag="I9573_002732"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAT4797341.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDEIGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(2181..2672)
                     /gene="leuD"
                     /locus_tag="I9573_002733"
     CDS             complement(2181..2672)
                     /gene="leuD"
                     /locus_tag="I9573_002733"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020005769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAT4797342.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKEKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2687..3964)
                     /gene="leuC"
                     /locus_tag="I9573_002734"
     CDS             complement(2687..3964)
                     /gene="leuC"
                     /locus_tag="I9573_002734"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAT4797343.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTITKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3921..5663)
                     /gene="leuA"
                     /locus_tag="I9573_002735"
     CDS             complement(3921..5663)
                     /gene="leuA"
                     /locus_tag="I9573_002735"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAT4797344.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIKGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     gene            complement(6181..6627)
                     /locus_tag="I9573_002736"
     CDS             complement(6181..6627)
                     /locus_tag="I9573_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797345.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6648..7367)
                     /locus_tag="I9573_002737"
     CDS             complement(6648..7367)
                     /locus_tag="I9573_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAT4797346.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYSGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGTRVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7485..8759)
                     /locus_tag="I9573_002738"
     CDS             complement(7485..8759)
                     /locus_tag="I9573_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAT4797347.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
BASE COUNT         2704 a         1738 c          896 g         3559 t
ORIGIN      
        1 cattttctgc cttttcataa gtctgaattt tctgaattat tctctcaaaa aaaatatact
       61 ttttatctat ataaattaat ttttttagta tgtaaatttg acgctattac gataaatctt
      121 tttagtataa ctaatttatt tttatcttgt atttttattg tatttttgaa ttttattttt
      181 tctaaatata aaatttatgt tttaaaatat aaaattttta tttaatcaaa gttcaatata
      241 ttccactttt tctttcgatt cttcaaaaaa ccattcttca ttagaaaaat caccttctgc
      301 aatttgtcca ataggcattt tcatatactt tccagttgct gtcactgcta tatctccatt
      361 tggtagaatt atttctcctg taccttcaaa taattttctg ttttctttag ttattcttcc
      421 aacaactttt atagtagcat ctgttggtac tggtttctta tatttcaact ctaaagatac
      481 tgttactccc catatttggt catcatttat acttacagct cttccaatag tctcatctaa
      541 tattgcagca gacatacctc catgaactct cccaggataa ctttgatgcc aatcctttgt
      601 attacaaatt gatacaagct ctccattttc aagttcataa aaatctgctt taagtcctag
      661 ctcattttgt gttccacaaa ctaaacaaga tttactacta ttttgcttct tcaaaacttt
      721 atactttgcc aaaaaatcca tctcctaacc gacattagta gtcgcttaat attaaataat
      781 tctaaaatat attatataat attaataaaa tccctgcaaa cttctaataa taaaaatagg
      841 ctacttcaat atgataagtt taatttaaag tattattttc caaaaaataa ctttatcaaa
      901 atagaaactc aaagtagata taagcctctt tttcgctatt aaaatagtaa aaatatattt
      961 gacttaatat aaaaaagagt gtctcattaa ctaataaagt ttattttgag acacccctca
     1021 taaaacaaat tatactacaa tagattttta ctaattctac aagttatata tttaaattat
     1081 attattcaaa ttaaactata attctaaatt tcatttacta ttaattcgcc cattgctctt
     1141 gttcctacaa catttcccac accattatag atatcaatag ttctataacc tttattgagt
     1201 acactttgta cagcatcttc aatacactta gcttcttctt ctaagttaaa actatgtctt
     1261 aacatcatgc ttacagatag aatagttgca attggattta caatatcttt tccagctata
     1321 tcaggcgcac ttccatgtat tggttcatac ataccaaaac tatcttctct aagtgatgct
     1381 gatggtaaca tacctataga acccgtaatc atagatgctt catcagatat tatatctcca
     1441 aatatattat tagtcactat tacatcaaat tggctagggt cttttacaac ttgcatagca
     1501 gcattatcaa catacatatg tgacagttct acatcctcaa actctttagc taagtcttcc
     1561 attatacttc tccaaagtct tgaactctca agcacatttg ccttatctac acttataagt
     1621 tttttatttc tatttctggc tatttcaaaa gctctcttac caattcttct tatttcattt
     1681 tcgttatata tttctacatc ataagcattt tcgataccat caatgatttt tcttccacgt
     1741 tctccaaaat aaatgccccc tgttaattct cttacaacaa caaaatcaat gcctttctca
     1801 actatatccg ttctaagtgg tgaagcttct tttatacttt catacattgt agctggtctt
     1861 aaatttgcaa ataaacccaa tgactctctt aattttaaaa gaccagactc tggtctttta
     1921 tctcccttac atttatccca ctttggtcct ccaacagcac caagcaatac agcttcgttt
     1981 tttcgacaag tttcaacagt tttattaggt agaggttctc caatttcatc aatcgcgcat
     2041 cctccagcta atacatattc acaatcgaat ctatgattaa atttacaaca aattttattt
     2101 agaaccttta taccttcatc tataatttcc ggtccaacac catcaccttt tatgactgct
     2161 atattacaat tcatactcca ctacccccta tttgctttta ttgagttaat taagccttca
     2221 tttaatataa tattttgcat aaactcagga aatggttctg ctttatattc tttttctttt
     2281 gttatatttt ttatcacacc tgtagaaaaa tctacttcta tattatcccc ttcatctatg
     2341 ttattggcag cttcttcaca ttcaagtata ggaagcccta tatttataga gtttctaaaa
     2401 aatattctag caaaagtaga agcaatcaca caggatactc cactttcttt tattacaatt
     2461 ggtgcatgtt ctcttgatga accacaacca aagtttttag ttgcaactat aatatctcct
     2521 ttttttacct tagaaataaa tttatcatca atatcctcca tacaatgcgt tgctagttct
     2581 ttataatcag ctatatttaa atatctagct ggtattatta catctgtatc tatattgtca
     2641 ccgaatttaa aaacacttcc attggcaatc ataaatttta cctcccttaa attatttctg
     2701 atggttttga tatttttcca gttatagctg atgctgctgc tacagctggg cttgcaagat
     2761 acacctctga atcaacatgc cccattcttc ctacgaaatt cctatttgtt gttgaaatgg
     2821 atttttctcc tgctgcaagt atacccatat gtccccctag acatggacca caagttggtg
     2881 tagaaactgc tgctcctgcc tctataaaag tggtaatata ccctttttct atagcttcaa
     2941 gatatacttt ttgagttcca ggaaatatca taactctaac tccatctgct acttttttgc
     3001 ctttcattat ctcagctact acttcaaggt ctgatattct tccatttgta caagaaccta
     3061 ttacaacttg gtctatcttt atatccttat ctatctcatc tactgttcta gtattttcag
     3121 gaagatgagg aaaggctact gtttctttta gttcggctaa atctatttcg tatacttcat
     3181 catagactgc atcttcatct gcttcatata tatttacatc ttttgttata gttgaacact
     3241 tatgtgaatt tatatattcc atagtcttat cgtctactgg aaagattcca ttttttgctc
     3301 ctgcttctat tgccatatta caaatagtaa atctatcatc catagataag tattctactc
     3361 catctccaca aaattccatc gacttgtaaa gtgctccatc aacacctatc tcccctatta
     3421 tatgaagtat tatatcttta ccacttatcc atttatttgg tttattcttc aaaacaaatt
     3481 ttattgctcc aggaacctta aaccaacatt ttcctgtagc catacctgct cccatatcag
     3541 ttgaacctac accagtagaa aatgctccta atgctccata tgtgcaagta tgagagtcag
     3601 cacctataat tacatctcca gcagttacta tacctttttc aggaagaaga acatgctcta
     3661 ttcccatatc tcctacatca aaaaaattta caactccttt tgattttgcg aaacttcttg
     3721 taaatttaca ttgttctgca ctctttatat ctttattagg tgtaaagtgg tctaaaacca
     3781 tcactacttt agtcttatca taaacatctt caattcctaa tttttcaaat tctctaatgg
     3841 ctactggtga tgttatatca ttacctaaaa ctacatctaa atctgcttcg attaattgcc
     3901 ctttcttaac ttcatttaat ttagcatgtt tggctaatat tttctgcgtc atagtcatcc
     3961 ccatttttta tttcctcctc aatataactt ttatttattg cattcataag tgcattaata
     4021 gctgctgtat ttatgttttc cgatatactt acaccaaaat agacttcatt attgtgctct
     4081 attcctacat aagctacccc tcttgaatgt gacccttcct ctaatgcatg ttcccagtaa
     4141 tatctaaatt tataatcttt catgccacat tgctttagtg cattattaaa tgaatctaca
     4201 ggtccatttc ctataccaac aatcctttgt tcatttccca tataacttat tcttgcagtc
     4261 atctcaatca tgttagtatc attagaatca tcattttcat agtttaattc atccattgat
     4321 tttattttat acttcttaac tttataaggg ctctcaactt caatatattc tttcttaaat
     4381 aaattaaata ttgcttcact actaagttct tccccaattc tatcagattc cattttgact
     4441 acatttccaa aatcagaatg catattctta ggtatcataa atccataatc tgtttccatt
     4501 atataaacag ctcctccttt tccagattgg ctatttatcc ttataatttc tttgtattct
     4561 cttccaatat catgagggtc tattggtaaa tatggaactt cccagtaatc attatctctg
     4621 tttttcattg ctatcatgcc ttttctaata gcatcttgat gtgaacctga aaaagcacaa
     4681 tgtacaagat tacctgcata tggatgtcta tcatgtacca taagttttgt acagtcttca
     4741 tatataccaa ttatcttatc tatattgcta aaatctagct ctgggtctat tccttgtgta
     4801 taaagattaa gtcccacatt tactatatcc atatttccag ttctttcacc atttccaaat
     4861 agtgttcctt caagtctatc ggcaccagcc aataatccaa gttcacttgc agccgtacaa
     4921 gttcctctat cattgtgagt atgcaaactt agtataatag aatccctaca acttatattt
     4981 ttacaaaacc attctatttg gtctgcatat acgtttggtg ttgccatttc cactgttgat
     5041 ggaagattta ttattacttt tttttgagga gttggtttcc aaacatctat cactgcttca
     5101 catatttcaa gtgcataatc catctctgtt cctgtaaaac tctctggtga atattcaaaa
     5161 gtaaattctg tttctggata tttttttact tcttcattaa ataattttgc tccttcaact
     5221 gctatatcta ttatttcttg tttactcata ttaaaaacca catctctttg aagaacagat
     5281 gtagagttat ataaatgtat aattgctttt ttacatccct ttattgattc aaatgttttt
     5341 tctataagat gagccctaga ttgtgtaagt acttgtatag ttacatcatc tggaatcata
     5401 ttctcatcta ttaattttct taaaaatgta tactctgtat ttgatgctga tggaaatcct
     5461 acctctattt ctttaaatcc tgtatcaata agcattttga acattctgac tttttcattt
     5521 acactcatag gcgttggcag tgactgattt ccgtctctta aatcgacact acaccaaatt
     5581 ggagccttaa atatttcatt atctggccat ttacgattta ctatttgcat tttatcatat
     5641 tttttatact ttccacactt catgttattt cccccttttt ctgttttttg tattcagtat
     5701 accttatatc gacgcaatcc tgcacacttc ataaggataa atgtcaattt tatcattaga
     5761 ataaaaaaaa cctcatctct aataaaatta gagacgaggt tttgctcgcg gtaccactct
     5821 atttagcttt ctaaaaagct cccttagtaa tagccaatac aataattata atgactatat
     5881 ttcctataac gtggaattac tccgattatt ctaagattta aatcttagaa aacagctcct
     5941 agacaagttc agaactatca actactaaat cacaccatcc tttagctctc tgaaagtttc
     6001 attgttctta ctacttctat tcatcacttt tttaatcatt taaattttct aaatattttg
     6061 atttaatagt tattatatac acattaaaat gtattgtcaa caaaaaaaat aattatctca
     6121 attttttatt tttgataagc aattctataa aacatgctaa ctattgaaaa cagtatgata
     6181 ttatgcctta aactttttca tcttttcatc tatgatattc aataattttt tttcatcttc
     6241 caaactcaat gcccttaatc taattttata attagattcg tcagtgtata tatttaatat
     6301 attttttact acttctattt tttctattat cttataatta aacttttgac tttgtttaga
     6361 ttttatactg aatatacatt gattatttat taatattgaa tgattatcga gagtaatata
     6421 tacttttccc tcatatttat ttgaaaaatc acgaccttgt ttaaatagaa aaactgctgt
     6481 tattatacat aaaatatata atattgtcgt tgaatatata aaaattgtag ctaaaattat
     6541 aagtgctaaa accatatata tagtcattcc ttttttaaaa taaccattgt cataataatt
     6601 catatagata aacttatttt tactcaaatt ttttcactcc caagattcta aaaatttata
     6661 tcaaaaaatt ctataatttc atcttcatga taatttatga ctaattcatc tggaaaatct
     6721 actgcttcta aaactttttc tgcattttca aattctccta tagaataaca tatatgagag
     6781 tcagtaccaa gaattactct tgtaccatat gttttacaca atgtcaacat atgacttaca
     6841 ttttcccaag tacccgttct atgagagttt gaacttaatg atgaattatt tatttctaaa
     6901 aggatatttt tatctttagc tttttttact ataggttcat aatctaaagg atacctactg
     6961 tcatctggat gtccaattat ttttacctta ggtttatcca taacttttaa aatagcattt
     7021 gtattttctt cttttgtacc agaagctata caaggtctat gcaaacttgc gattatataa
     7081 tccaaatgtt gcaacatatc ttcttgaaca tctaaattac cactataatc cattatattt
     7141 ccttctattc ccctaagaat ctttacacct tgcacttcac gtggaagcaa atgcaaatta
     7201 taaaaataga atggatgtgg tcctcctggc atattaggtc cgtggtctga tattcctaag
     7261 tattttatat tcttcttact cgcctcttcg acattttcct tgaaagtact atgtgcatgt
     7321 ccacttatta gagtgtgaca atgtacatcc ataatagact ttttcattaa atttcctcct
     7381 aatattttac ataaattcgt gaaaatagct aaagggctat ttcaaaacta aagctgtcat
     7441 aatagacagt gtaacgtttt aaaacagccc cattttcaat tattttacac ttctaaatct
     7501 ttaaatttat ctttaaataa ttctccaata cttcctaaag attcctctac ttctaggtat
     7561 tctgttatat cctcttctgg ctctctagtt gcttctttta ttgaaaccaa cattctttta
     7621 gtctttggtt taaaatcaag aatcttaact ttaacctcat ctccaatatt aacaacatta
     7681 gtaactttag ccactctagt atcactcaat tcagatattg gtaaatacgc atctacacct
     7741 tctttaattt gaacaaaagc ccctttttct attattctaa gaactttagc attgacaaca
     7801 tcatcaattt gtacttcttt tgctattaaa tcccaagggt cattatttat gtcttttaat
     7861 gccaaggcaa tttttttagt ctctttattt acatccaaca cataaacttc tacttcttgt
     7921 ccctctgaaa caacatcttc aacactttca actcttgtcc aagctaaatt atttatatgt
     7981 gctaatcctt caatcccacc aatatccaca aatgcaccat aaggcattat ctttgtcacc
     8041 ttaccatctc ttttttgtcc aacttcaaga gaattaaata agtcttcttt agctgctttg
     8101 atacgagctt tttcttcttc tctttcttgc ttaattcttg ctctttcagc ttctttttct
     8161 gccttaattt gagctcttct ttcttctctc tctttattta tacgctcttg taatacatct
     8221 ctatgagatg ctacaagtct attttttcta acatccaatt cttttatata aacttctaag
     8281 ttaagaccaa caaatttaga tgtatctgta ataaattttg tatcaagttg agaaattggt
     8341 ataaaaaatg tatatgtatt gtaattagca aatacacctt tatctatatt cttttcaaca
     8401 tttacagtta ttattctatg ttcatcataa gccttttgta actcggcaaa atcatttctt
     8461 ttgtcaagtt gtaatttaga taaagttatt gtaccatctt tttgagatac ttttgttatt
     8521 acagcagtta tttcatcacc tatgtggtat atatcttcta tatattgatt tttttctatg
     8581 ttcaattcac ttataggtat tattccatca aacccatagt ctaatcctaa ttgtacttca
     8641 tcatttctca ctgcagttat ttttccagtt gtcaattctc caactttcac tttgctgaat
     8701 acttgttctt gctggtcaag taattcttgc attgttaaat cattttccat tataaacatc
     8761 ctttcctctt attgattatc atatctgtaa atattatacc attaatattg caaatactta
     8821 catttttctt actataattt ttaaaataaa ttcaacttca tatatttata cattatagaa
     8881 aatatcttat taaatta
//
LOCUS       DACTGW010000159         8814 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.159, whole genome shotgun sequence.
ACCESSION   DACTGW010000159 DACTGW010000000
VERSION     DACTGW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.159"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            350..580
                     /locus_tag="I9573_002739"
     CDS             350..580
                     /locus_tag="I9573_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HAT4797348.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            593..742
                     /locus_tag="I9573_002740"
     CDS             593..742
                     /locus_tag="I9573_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HAT4797349.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            999..1436
                     /locus_tag="I9573_002741"
     CDS             999..1436
                     /locus_tag="I9573_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HAT4797350.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            1693..2586
                     /locus_tag="I9573_002742"
     CDS             1693..2586
                     /locus_tag="I9573_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAT4797351.1"
                     /translation="MQLYNTPFNTNKHLIYFFTSTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            2764..3588
                     /locus_tag="I9573_002743"
     CDS             2764..3588
                     /locus_tag="I9573_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT4797352.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKHEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREVVPDYQSEGILWEKLSKFIKEEHIE
                     VSRQPNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            3714..4295
                     /locus_tag="I9573_002744"
     CDS             3714..4295
                     /locus_tag="I9573_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT4797353.1"
                     /translation="MKADSTKEQLINATIQLLSSHRDVSSITAREIVAKANVNLAMIN
                     YYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFDFIGEHSQFI
                     KISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSFIQLLFLRSN
                     AFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            5871..6623
                     /locus_tag="I9573_002745"
     CDS             5871..6623
                     /locus_tag="I9573_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797354.1"
                     /translation="MLKVNQLCKSYKTGNKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            6613..8724
                     /locus_tag="I9573_002746"
     CDS             6613..8724
                     /locus_tag="I9573_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAT4797355.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYI
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVAGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIERVYMKKSSYDMFFNSK
                     DFSKLIDKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         3372 a         1138 c         1144 g         3160 t
ORIGIN      
        1 ctgatactaa caaacaaaac actaatattg acaattgaaa tactaatact aacaagcaaa
       61 acactaatat tgacaattga aatactaata ctaacaagca aaacactaat attgacaatt
      121 gaaatattaa tactaacaag caaaacacta atattgacaa ttgaaatatt aatactaaca
      181 agcaaaacac taatattgac aattgaaata ctaatactaa caagtaaaac attaatattg
      241 acaattgaaa tattaatact aacaagcaaa acactaatat tgacaattaa aataccaata
      301 ttaacaagca aacaattaaa atataaatta ttatatcgga ggtaattata tggaaattaa
      361 gaaaaaatta gtcatgagct ttaaaaatgt aaaggataaa caagtttctt tttctataga
      421 aaatccaaag gatgatttaa ctgaagaaaa tataaaatct gtcatggatt tgatagtatc
      481 taaaaatatt ttttcggttg gaggatttga cctagcttct gttgttgaag ccaaggttgt
      541 ggaaacaaat accactcctt atgacttagt tatagaatag ggggctaatt ttatggatga
      601 tagtttacaa ctgttaataa ccaatgttgg ttttcctata gctctatcca tgtacttgtt
      661 ggttagaatt gagggtaaat taaatacttt atcaaacagt attgatacat tatccaaaaa
      721 tattttgata tttaagaagt aatttgacaa tatatgtaag tcataaaata ggtctatttg
      781 taaaataatt agacatattc tatgctaaat actaggatat ttggataatt tatccaaata
      841 tccatagtat ttttttgaat tttaccacct caaatttgaa tttcactatt ttatttttga
      901 atttcactat gcttaatttg aaatttagaa cgcatctagt tggactcaat aagatataat
      961 gctattctaa tgacagtaat aaaatgaggt gatgattaat gaaaatacat gtcgaacaag
     1021 gtattaattg tgatgatatt gaaataacca taaaatgttc tgctctagat aataacattg
     1081 aaaaaataat ttcattattg agtacaccat tacttgatat aaaaggtaaa aaatcaggag
     1141 agatttataa actcaatgtc tgtgatattt tatactttga aacagttgga aataaaacat
     1201 ttgcttatta tgaagatgat gtatttgaaa ttgatttaaa aatatataaa ttatcaaaat
     1261 tattagctaa tacatccttc tcacgaatat ctaaatccat gattttaaat atggactaca
     1321 taaaaagtat aaaagtaata tcacataatc gcatgcaagc tactttaaaa aacaacgaaa
     1381 aagttattat aaatcgtcgt tatataaaaa atattaaaga aaagctaaac atttaataaa
     1441 aatctaaagt aatttattaa tatcactaat tgcaaattta ttgacctatt taataattaa
     1501 taataatata atatgttcaa tattaaatac taattaatta tatttagtgt aaaaataatt
     1561 aatcaaaata taaaaaatag gatattatta taggcaataa ctaacatcaa acgataactt
     1621 aatacatata aaaataaata aacaattaat aaaaattaaa acttcattat aaaacggaag
     1681 gatagtgaaa aaatgcaact atataacacc ccctttaata caaacaaaca cttaatctat
     1741 ttctttacat ctacgctagt attgaactgg atattcagtc ttacacctgc gttgctcgga
     1801 attacaaata caatacttgg agaaatcatt ttttatcttg catatacatc accagctatt
     1861 gtaggtctat tttttatttc tacaatctat ccacaaagtg caaaacaaga ttttatttat
     1921 agatacctaa gctttaaaga aattagaata aaatggatac tattaacaat attatttttt
     1981 gctttaactt ttattacttc gttaataatc ggaaattact ttaatgtaaa aacttctgat
     2041 atagattgga tacacatagc agtgtttaga ccttctaaaa tagcttatat gctattagca
     2101 agttttatat tgagtatact tagtcaagaa gctggatgga gaggatacgc cattgacaaa
     2161 ttattagtta gatttggatt tataggctct tcaattattt tagggcttgt ttgtggaatc
     2221 tggtatctag ggtcgtattt tacacctaat caaataccat ataatttggc acaatactcc
     2281 ctatttgatg cttttttatt tataccaaac attgtacttt taaactttat aattaatttt
     2341 gtatatatca atacaagtag aagcatttta tcagctgggt tagttcatat gatgtactat
     2401 tttttcaaca ttcaattact tctacactac cctattaaat tatgtgtaat agctcaatat
     2461 gtacaaataa tatttggttt aatatttctt gtatatatga tttcttctaa taaatttaaa
     2521 cagaaggtaa attctgaaat tgaacagata aagtcggatg attttgaatt tgagttagac
     2581 tggtaatttt aatatgaatt ataaattaag ataatctatg atttgatacg attactactt
     2641 aaatataatt tactctctat aatttattgc aacatctgga aaataattgt atttccacaa
     2701 tcgcttgaag tatataaaaa caaaatatat actaattata agtgaaaagg aggtcatatt
     2761 ttcatgttta aaataggaga ggtttcaaaa ctaacacaaa tttctattcg tatgttgcgt
     2821 tattatgatg aacttggtat tttaaagcct gcagaaatag acaaatatac aggtcataga
     2881 ttatactcag ttgaacagat ttcaatatta caaagaattg tattattgag agattcaaaa
     2941 tttagcgttg cagaaatagc aaatatcgta cataattgga atgatgaatt tgtaattaaa
     3001 gaattgaata gaaaaaagaa tgaaatacaa aaggaaataa agcacgaaca acaaagaata
     3061 aataaaattg ataaatttat agaagctata aactgtgata aagatgaaat tcattacaat
     3121 gttgtcttta aaaagattcc aagttataaa atcatatcac ttagagaggt agtcccagac
     3181 taccaatctg aaggtatact ctgggaaaag ttatctaaat ttattaaaga agaacatata
     3241 gaagtatcac gtcaacccaa caataatata gctttttacc atgatgaaga agtcaaagat
     3301 aatggtgtag atattgaagt tggaatggtt gtaaagaaga ttggaaagaa caaatctgga
     3361 tttatatatc gtgagacaga agaaattgat atgatggcat gtactatggt ttatggtcca
     3421 tatgaaaata tagctggtgc atatgaatcg ttctgttatt ggttagataa aaatagtgat
     3481 tatcaaatat caggtataaa tagacaaatc ggtcataagg gtgagcataa tgaaataaat
     3541 cctgaaaatt atcttacaga aattcaaatc ccccttataa aagtctaatc gattagtact
     3601 taaatactta agtactaatc acccattgac atcccaaaat tttcatgata gtatctaaac
     3661 gtgtattaaa catgtttaat ttttttatgg agaaactttt aaggggaaaa attatgaaag
     3721 cagattcaac aaaggaacaa ttaattaatg ccacaatcca gttactatct tcacatagag
     3781 atgtttcaag cattacggct agagaaattg tagctaaagc aaatgtcaat ttagctatga
     3841 ttaattatta ttttaaatct aaagaggagt taattaatat ttctattaaa aaatttttaa
     3901 agcgtcatat ggatgaatat aaggctacac atgatactaa aaatttagca cctaaacagc
     3961 aattaaggga aatactaatt gcattttttg attttatagg tgaacatagc caatttataa
     4021 agatttctgt accctatata ttgatacaag aagaaattgt ttatccattt gagatactac
     4081 ctttaattaa ggctcactat gaaaatcaga ataacaaaaa aactgaagct gaatacagaa
     4141 ctattgcgta tcaaataact tcatttattc agttgctttt tcttcgttct aatgcttttt
     4201 tcaagtatag tggtattgat attcttaatg ttgaacagag aaatggttta atagattttc
     4261 aattagattt attattggta gatattacaa aataatacta tattaatttt ttatgtacat
     4321 ttttttattt tatggtaacg tttgaaaata ttctcattga ctaaaaatac ttgcattaaa
     4381 taagggggag gaattttaat gttttttaca tttataggag taatatcagc aattttattg
     4441 ttgtgtacaa ttatatgtgg tttatggata aaacttaata atcgtaataa tgactctaat
     4501 tctcacttta ctttttcaat actttcaatt gctttttcac tgttaattgt ggtggccttt
     4561 ttagtaagaa attaatgtct ttgttatgct ggggtttatt attatagatt tttattattg
     4621 catgattttt actatattat agtattttta ttatattaca gggtcttatt tattatagtg
     4681 ttttgttatt acagcatttt attatattac agagtcctat ttattatagg atttttatta
     4741 tattgtaaaa tcttatttat tataggattt tgttatatta cagactttta ttatattaca
     4801 atctcttatt tattgcagtg ttttgttatt acagtatttc tattatattg caaactctta
     4861 tttattatgg tgttttgtta ttgcaacatt tctattatat tataaagtct tatttattac
     4921 agttctttgt tatactacag acttttatta ttacagcatt tctattacaa tctcttattt
     4981 attacagttc tttgctatac tacagacctt tattattgca acatttctat tacattgcaa
     5041 agtcttattt attacagttc tttattatac tacagacttt tattattaca acatttctat
     5101 tatattacaa tctcttattt attacagttc tttattatac tacagacttt tattattaca
     5161 gcatttctat tacattgcaa agtcttattt attacagttc tttattatac tacaaacctt
     5221 tattattaca acattttatt attttaacgt ctttactata atatagtaat attttaatat
     5281 ctcaaaatca tatcaatcac ttttcagcat agtctaaaat acttccactt tctaaaatta
     5341 tctatcatac ctatactaat aatacttttt aaccttttta ttatacattt caattgtttt
     5401 taacgaccta tttatttcca aaactccaat atattttctc aattacattg tgttaataac
     5461 ccctaaatcc tttcaaatta tttacaatac attccattaa ataatcttac tgctctataa
     5521 ttcttttttt ctttttaacc ctactataca ttatttacat catttttttc aatacaacat
     5581 tcgttattct ataacctttt tacttttact atataccttt tttctaacta caccttcaat
     5641 gttccacatc atattacttt agcacaacaa ttaataccta aaaataaccc tctaaatcct
     5701 ccaataatat aatcaattta actataataa taacctctca cactatccaa acaatatatt
     5761 ttcaaacatt acaaaaaaac atccaactat tacaatattg ctcatatccc tcctcccctt
     5821 tcaaatgcta tcattgactt acaagataaa tgaaagtgag gatgatatat atgcttaaag
     5881 taaaccaatt atgtaaatca tataaaacag gcaataaaac atatcctgta ttaaaaaata
     5941 tttcttttga aatcaacaaa ggagagtttg tcgcaatcat gggttcatca ggaagtggaa
     6001 aaactactct tttaaattgt atttcatgtt ttatcccaat tgataatggg gaaataattc
     6061 ttggaaataa taatataaga aatttaaaag aaaaggaact ttcagaagtt agaaaccaac
     6121 aactaggatt tgtattccaa gatttcatgc tatttgatgg tcttagtgtc tttgaaaata
     6181 tatgtgttcc acaaattatc aaaggtagtc aagtcttaca aatggaaaac cgtgcaatta
     6241 atctatgcga gctatttggt attgaacata tcagaaaaaa ataccctgcc gaaatatctg
     6301 gtggagaaaa acagcgtaca gcagtagcaa gagcactcat gaacaaccca gatttaatat
     6361 tagcagatga accaactggt aaccttgata gtaagtcttg tcgtacagtc attgaatcat
     6421 ttttaaaagc aaagcaagaa ttagatgcta ctatactcat ggttacacac gatagctttg
     6481 cagcaagctt ctgcgaccga gttattgtgc ttaaagatgg aataatttat aaagaagttg
     6541 taaaatcaaa ttcaagacat aaattcctag atgaattact agaagtttta aaagaaatgg
     6601 gaggcgctca agatgacaat taataaatta tacagcaaac ttcgagccta taattggaag
     6661 aactatacaa ctttattctt ttgtataata ctttctgtgg tattagtaac ttcatatgct
     6721 ctaatttatt ttagcccaac aatacaaaac atacttccaa ctggtggaga tagcagaaaa
     6781 caggctacaa tgatattctc agttgctata ttaggatgcg ctgtctttac tacatacgct
     6841 tcttccctct ttttaaaatt taaaagcaag gaaattggta ttttcatggc tctaggaaca
     6901 gataagaaga aattaaaaaa acttctattt tcagagatat tgttaatttc aatagtatgc
     6961 tgttttttag gtcttatatt aagtattcct gtatcttttg gtatatggaa aatatttcaa
     7021 ttagtgatta ttgataccag agaaatgaca tatcattttg gaactcaagg attcatatat
     7081 ggatttatat ttagtgtatt tgtgacatta tgcatattta tactttctgt aaaatttatt
     7141 aatcgtacaa atataattga tatattaaat gaccagagaa tatctgaacc aatacaggat
     7201 gtaaaggact ggtatggtat attaggtata atttttatta tactaggtat ttttctagga
     7261 tatatcgtac cttcactaac tataaacaaa ttaaattata gactaccatc tgtatggagc
     7321 tttacttatc tactctctct tattggaata tatatgatta tgacatacat agttgtacat
     7381 gctaaaagag gaaaacatcc agaaaaatac tataaaaaca tcatatcaac tagcatgatg
     7441 agatttatgg gaagacaaac agtaaaaaac atgtgtgtta tttcattttt gattgcagga
     7501 gcattatttg ctgtattcta tgttcctaat atggttgcag gtatctatga tactcttaaa
     7561 aataacccta tagattatac ttttacctac aatcaaagag ataaacaggt atcaaaaaaa
     7621 gatattgaac aattagcaga taaacacaat ataaaaattc aaaactatat ggaaattcct
     7681 tctatagaat taattgttga tggtgtagaa tcaatatatc ataaagatgg aactatcgaa
     7741 agggtatata tgaaaaaaag ctcctatgat atgttcttta attctaaaga tttttccaag
     7801 ctaattgaca aaccaataac tataaaaaat ggtgaatatc ttgctgttgt aaataaagga
     7861 aatgaaaatc aagattataa atataccaaa tttatatcaa gcccagtcac agaaaaagaa
     7921 atccccttta gatatgctgg aaaaataata tttaatggtt ctttttttca aaatagaagt
     7981 atgaacgcct atgtccttaa tgatgaggat tataaatcat ttactaaaga tttatccata
     8041 aaaaattaca ataattctat actatttaat gtagataaag atacctatgc ttttgccaat
     8101 gacttaaaga aagaaattat aaaaagaaca tctaaggaaa tgggcatgtt cagtggttat
     8161 gatgaatacg taaaaaaacg ttatgaatca actggaagaa aatattttat ggatgaagaa
     8221 tatccaagtg gtgaaggtca tttaaaacta gacccaaaca acaatcagtt gtatcttaat
     8281 tggaaatact atcctaactt taaaatttta caaagccaag atatgataaa aaacatggca
     8341 gtcttcttaa tgttattcat atatgtggca atcatatgtt ttacagcagt aggaatcatt
     8401 gcatatacaa ggggtgttac tatagctata aatcacaagc aggtctttat ggatttaaat
     8461 cgattaggag caaacaatga ttatataaag ttctgtatta gaaatcaatt aaagaaagta
     8521 ttttcatacc ctcttattgc aggaagtttt ttaatttatc tgtttaattt tatgattatg
     8581 ttaaataatg atggtagaat cgtcaatagt gaggtcaatg ctcttttgat aaacttaggt
     8641 tttataggga tttgtggagt ttatatgtac cttatatatc tattaactta taagaaattc
     8701 aaaaaaatta ttggtatttt ataataagga aggttgttag atttttaaag taataaaatc
     8761 attacttttt ataataagaa aagcattagc attttaaatt aaaaaaggtt atta
//
LOCUS       DACTGW010000160         8743 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.160, whole genome shotgun sequence.
ACCESSION   DACTGW010000160 DACTGW010000000
VERSION     DACTGW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.160"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            73..414
                     /locus_tag="I9573_002747"
     CDS             73..414
                     /locus_tag="I9573_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HAT4797356.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            565..978
                     /locus_tag="I9573_002748"
     CDS             565..978
                     /locus_tag="I9573_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HAT4797357.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNLYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1180..1821
                     /locus_tag="I9573_002749"
     CDS             1180..1821
                     /locus_tag="I9573_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAT4797358.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1845..3098
                     /locus_tag="I9573_002750"
     CDS             1845..3098
                     /locus_tag="I9573_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAT4797359.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEKENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLREIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3144..3794
                     /locus_tag="I9573_002751"
     CDS             3144..3794
                     /locus_tag="I9573_002751"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAT4797360.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGVDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3845..4441
                     /locus_tag="I9573_002752"
     CDS             3845..4441
                     /locus_tag="I9573_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAT4797361.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4454..5293
                     /locus_tag="I9573_002753"
     CDS             4454..5293
                     /locus_tag="I9573_002753"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAT4797362.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFNNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5538..6692
                     /locus_tag="I9573_002754"
     CDS             5538..6692
                     /locus_tag="I9573_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797363.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKFYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            6998..8695
                     /gene="ade"
                     /locus_tag="I9573_002755"
     CDS             6998..8695
                     /gene="ade"
                     /locus_tag="I9573_002755"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAT4797364.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIASLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3581 a          841 c         1568 g         2753 t
ORIGIN      
        1 atatatttcc gatagattat attgataaaa aacactaaaa ttaaattaat aaaagtactt
       61 ggagggatgg aaatgaaaga ggaaaatttt attaatgttg aatacgagga taaacgtttt
      121 tcaattaata gtaaagaagt tttgtacata gaaaagcaag agtatgaagt aaagatttat
      181 actgaaagtg gtaattatga agttggagaa aatgctcctt gtataagaaa actattgaaa
      241 gaagaaaatt ttttcaaatg cagtaatagt tatattgtaa atctagacaa gtgtgaattt
      301 tatctcagca ataattgtat ttttgtgaat aataatttta taagaatttt aggttcagaa
      361 agaaaaaaga aattaaaaaa gaaattagaa aaattatatg attatgaaga ttaatattgg
      421 gaggctgtat tttatacagc ctctttattt attgattgta ttattaataa attttaaatt
      481 atgataaaat ataaaacaag taacttattt cagaataaaa tactttttaa aagaaaggga
      541 cgagatatgg gagattataa taaaatgatt gaattaaaga ataacttagg tatcagtgaa
      601 agaaagttaa aatatccttg tatctataaa cattttaaag ggaaatatta tgctacaatg
      661 ggtcttagca aggctattga tgatatagaa aacatatgtg aaatatatgg taaagaaaat
      721 ttaatccaaa acagaaataa atataaatta gttattagac atactgaaag agaagaagat
      781 atctatgttt atagggattt agatggaaac ctttatcata aaaaagaaga agatactaat
      841 gatttagttt tatacaagac cttatatgat gatacaggaa tatttgcaag acctttggat
      901 atgtttttag aaaaagtaga tacagataag tatgtcaatt caattcaaga atacagattt
      961 gaagaagtat ataaataata taagagaaat atgtttataa atttagaaaa attctttaac
     1021 agtacaataa tttgcagaaa tacaaatgag atttattgta aataatatgt tagattagat
     1081 tataaaatag aaacaaagta aagtattata tgatataatt aagttttgag aagtgataat
     1141 ttatatttaa taaaaaatag gcatatatta ggaggacata tgggaaatac tttttatata
     1201 gtaagacatg gacaaacgga ttggaatata ttgggaaaaa cacaaggtca tggtaattct
     1261 gaccttacgc ctcaaggtat agaacaggca aaagaacttt ctgaagatat tggcaagtat
     1321 tcaattgatt atatttttag tagcgacctt gggagagcaa tgcaaacagc tcaaatttta
     1381 ggagataagc tcaatattga agttcaaaaa actgaggcac ttagagaaat gggattcgga
     1441 gtttgggaag gccttttaat aaaagaaatt caaaaagatt attcagatat atatgcaaca
     1501 tggagaaatg aaccacactt agtaaatata ccagaaggcg aaactcttaa gattataaag
     1561 gaaagagtag atgcttttat aaaggaacta aatgaaaaat atgataataa aaatataatt
     1621 cttgttacac attcaattac attgagggtt atgttgttgt catttttaga atcaggtatg
     1681 gaaaatatat atagaattaa gcaagataat actgctttaa atatagtaga atttaaagat
     1741 tatggacctg ttatagtaaa aatgaatgat acaagtcata taaaaaatca tgtaaagata
     1801 aataattctg cactggagta ggaatcaatt ataaggagaa atttatgagt aaagttatag
     1861 tagttggtgg aggagcttct ggaatgatgg cagctttgtc agcttcaaaa aacaataatg
     1921 aagtaatatt agttgagaga aatggagaat taggaagaaa acttagggca actggtggcg
     1981 gtagatgcaa ttttaccaat aatagagaga tagaagattt ttttgataaa gttgtcagta
     2041 ataaaaaatt tctctatagt agtttctata catttacaaa taaagacctt atttcgtatt
     2101 ttgaaagtag aaatttggaa tataagattg aaaaagaaaa tgaccacaaa gtatacacta
     2161 aaaatgataa atctatagaa gtaatagaag tattaaataa agatttactc aatcacaatg
     2221 taaagataat gtataataag aaagttatag atattataac tgaagaaatt gctctaaaag
     2281 atgattctaa taaggacaag tcaaagtatc taataaaagg gattatttta gataatggag
     2341 ataagatact aggagacaag gttattatat caactggagg agtgagttat tcaaagacgg
     2401 gttcagatgg ttcaatgtat aagattctta aaaagcatgg acatacatta aacaaactat
     2461 atcctgcttt agttccactt acaatagagg aaaaatggat taaagattta caaggaatat
     2521 ccatgaagaa tgtggaaata tcttgtaaga ttaagaaaag gaaaatatca aaatctggag
     2581 acatgctatt tgcccatttt gggataacag gaccttgtgt tcttataatg tcttcttata
     2641 ttaacaagat tattgaaaag gaaaaggtag aattaaatat agatttctta ccaaatttaa
     2701 gtactgatga aatctctagt ataatcagag catttccaaa taaaaatgtt ctaaataatc
     2761 ttaagcaaat actacctcaa aatttcttaa gagagatttt ttctctatta tctttagttg
     2821 ataaaaaggc aagtgatttg tctaaggcag atgaaattag aattattgag tatataaaaa
     2881 atatgaagct aacttgtaat gggactacag gtataaacac tggtatggtt acttctggtg
     2941 gtatttcagt taaagaaatt aattcttcta ctatggaatc taaattagtt aagaacttat
     3001 tttttacagg tgaagtaatt gatatagatg cagaaactgg tggatataat ttacaaatag
     3061 cattttcaac tggatatctt gcgggtataa gtgtataaat ataatttttt aataattaaa
     3121 tatgaataaa tagaggtgta tatatgggta atttagttat agctgtagat ggtccagcag
     3181 gagctggaaa aagtacaata gccaagatag tagcaaaaaa gctaaatata aattatatag
     3241 atacaggtgc tatgtacaga gcagttactt ataagtgttt aaaaagtggt gttgatgtca
     3301 ataatgaaaa agaagttatt caaatagctg aaaattcgga tatagatttt aaagataaca
     3361 atatatattt ggacaaagaa gttataaatg aagaaataag aacaatagaa gtaagcaata
     3421 atgtttctaa tgtagcaaaa ataaaagaag taagacagtt gatggttgaa gttcaacgaa
     3481 aaataggaat gaaaagttca gtaatactag atggtagaga tataggttca tatgttttcc
     3541 cagatgcaga ttacaaattc tttttagtag ctactccaga agaaagagga aatagacgct
     3601 ataaagaact ttgtaataaa ggatataata ctactttaga agaagtaatt gaggatataa
     3661 taagacgaga tgaaattgat tcaaatagag aatttgcacc attggtaaaa gctaatgatg
     3721 cactagaaat agatactaca ggaaaaacta tagaagaagt tgtagaagaa gttgtatcta
     3781 aaataaatct ttagtttttt atgcatcata atgaaaataa atgtagtgtt gaagggagat
     3841 aaacgtgaat ttttatagat ttgtaataaa tatttttaag ggtttttcta aaatattttt
     3901 taaatatgaa gttataggtg cagagaatat tccagataga ggaaatatag taatagcatc
     3961 aaatcataaa tcgaatcttg accctatatt cttggctgct gccatagaaa atagggaaat
     4021 agctgcaatt gcaaagaaag aattgtttaa agtaaagcca ttgggattta tattaaaaaa
     4081 attgcatgtt atgcctataa atagagaaaa accagatgta tctacaataa aaactatttt
     4141 aaggtcagtt agagatggtt atgtattagg aatatttcca gagggtacta gaataaaagg
     4201 agattctttt ggtaaggcta aggcaggtct ttctgtattt acaataaaaa gtaaatctaa
     4261 agttgttcca gtatcaataa tatctaaata taaacttttt agtaaagtaa ttgtatatat
     4321 tggagaacca atatcatttg aagaacattt taaggaaaaa ttgagtaatg atgaccatga
     4381 aaggatatca caagagattt tagaagtgat aaaacaaaat tactttaaat attcaaaata
     4441 agggggtaaa gttatgaaca taaaaatagc aaaaaatgct ggcttttgtt ttggagtaaa
     4501 aagagcaatg aaaatggcat gggatgaagt tgaaaaaaat gattctggca tatatgcttt
     4561 aggtcctctt attcataaca agcaagcagt agcaaaatat gaagaaaaag gtcttaagac
     4621 tgtgaatgaa attgatacaa taccaaatca tgaaaatatg ataataaggt ctcatggagt
     4681 tcctgaaaat atatataagg aagccaaaga taaaaaatta aaaattgtgg atactacatg
     4741 tccatttgta aagaaaatac atacagtagt aagtgaatat cataataaag gctatgaaat
     4801 aatagttatt ggagatatga agcatccaga agttataggt ataaatggat ggtgtgaaaa
     4861 ttcagctata ataataaaga ccttagaaca aatggaaaat atggaattta ataactccaa
     4921 aaaatattgt cttgttgcac agactacaat aaatccagag ttgtatataa gtatagttaa
     4981 taaactatca gataaattag aagaaatagt atttaatgat acaatttgtt cagctacaaa
     5041 aacaagacaa gaatcagcta aagagttagc taaagaagtt gattgtatga tagttatagg
     5101 aggtaaacac agttcaaata ctcaaaagtt agtaaaggtt tgtgaagact tagtaccaac
     5161 ttttgcaata gagactaaag atgaattgga tgtaaatacg cttaaaaaat ataaaaattt
     5221 gggtattaca gcaggggcat ctacacctaa ttggataata gaagaagttg taacattcct
     5281 tgaaaattta taatttgata ggtaataaaa ataggtggac attgttataa tttatcataa
     5341 tatccacctt tgatttcata ttacatgtgg taaatagata acctaatatc aaaaagtgtg
     5401 tatcttaaga tgaaaaacta agagtataag atgcgcactt tttaatatta aaaattcaaa
     5461 agaaatattg atattgatta tcaatagaaa atagaatata atataattgt tgcaaaatta
     5521 gtctaggagg taaattaatg gaaatgttga tatccgcttc tgaagaggcg tttttacatg
     5581 taggttctat gattggattt tttattttgt tatttggata tattaattat aaaactagtg
     5641 gaaattttac taatatcata tcaaaaaaca gaaaatttca acccttaata ggtgctttaa
     5701 ttggagctat accaggttgt ggcggaagtc tggcaataat gccattgtat ataaatggaa
     5761 aattaagctt tggagcaata attgcaagtt taatagctag tatgggagat gcagcatttg
     5821 tacttatttc atcaaatatt aaaatgtatt tttttgtcac tatagttagt acaattacag
     5881 gaataataac gggacaatta gtggattact ttaaactaga agaaaaatta gggttaaaaa
     5941 accgtagaaa atcagataaa tatgatataa gtaaaaataa ccctaaagaa aataaagaac
     6001 aacataatca tgatgaaata attttagaca ctctagctaa atctcatgga aatacaaata
     6061 gacttgcatt tataataaca catggaaagg gatataaatt ttatatagga attatagtaa
     6121 tcggttttat atttatgtca cttgctcact ctggacttaa tcttcctata attgagaagt
     6181 tacattcatt ggaagaagta atagcagtga ttggaatatt attttcaata atttatatgt
     6241 ggtgttttaa aaaggtattt aaaaattcta atcaaaaaga ggaagaaaat aaaaagattt
     6301 ctttgcgaga aatgcttatc cattctgttg gagaaatatc atttgtaatt acatggattt
     6361 ttattgcata tcttatttac gatttgataa tattaatttt aggtggagat gaatacttag
     6421 ttaaactagt attatcaaca ggtgtaataa gtgtatttat aggtgcaggt cttggactta
     6481 ttccaggttg tgggatacaa attgtgctta tgtcatttta tttgaaggga actataccac
     6541 ttggtgcagt gatagccaac tctatttctc aagatggaga tgcacttttc ccacttttag
     6601 caatggataa aaaatcttct ttatgggcta tgattattac tactatacca gcaatacttg
     6661 taggtatgat agtttatgca tttttaggtt aaatataatt acgaataaag aagtattaaa
     6721 aaaataataa agcacataaa tttatttata agaatagtga agattaaata taaaaataaa
     6781 aaattcaata tgaattaaat tattgcttaa atattaatga gaacatggga gataatatta
     6841 ttcctcatgt tctctctatt taattaaagg aattatggaa caaatttcaa gtgatacttt
     6901 ctatcaaaac tcatatataa tatatattaa taaattaaac tataatacgt aatacagcca
     6961 gtatattgat ataaaaaact taggaggagt tgtaaaaatg ggtaatttaa aaagacagtg
     7021 tgatgtttca agtggaagag aaaaagctga cattgtacta aaaaatggga caatcataaa
     7081 tgtatttaca gaagagttaa taacaggaga tgtagcaata gtaggagaca ctatcgttgg
     7141 cattggcgat tacaagggca atgtagaaat tgattgtagc aataaatata tatcacctgg
     7201 ctttattgat gcacatatgc atattgaatc tacaatggta atgccaattg aattatcgaa
     7261 aaaattatta aaatcaggta cgacaactat catagccgac ccacatgagt tggtcaatgt
     7321 aaaaggagct agtgcaatag attttttatt agaatcgaca aaagatatac cacttaatgt
     7381 atatattatg gtgccttcat ccgtacctgc aacaagtttt gaaactaatg gagttggtaa
     7441 atttagtgca aaagacatgg agagttatgt aaataatcct agaattttag gtttaggaga
     7501 ggtaatgtgt tttaatgatg ttataaattc tgaaaatgaa atcttggata aactagagct
     7561 ttttaaaaat aaagttgtag atggacatgc acctaatata aatggtaaat cacttcagac
     7621 atatgtatgt gcaggaatag aaaatgacca tgaatgtata acttttgatg aagtatatga
     7681 aaaattgaga gcaggattaa aaattttaat aagagaagga agcgcagcaa aaaatttaaa
     7741 gtctatagta agtggtatgc taaagcataa cttgccaatt gaagaattta tgttttgcac
     7801 agatgataag cacttagatg atatagaaaa acaaggtcac ataagatgga atataaaatg
     7861 tgcgattgat ttaggtatgg aacctgttag ggctataaaa gttgcaactt ataattctgc
     7921 aaaagcatat gggttaagga aaattggagc aataggtgcg ggttataagg cagatattgt
     7981 tgtattgaat gatttagaca aaatggaagt ggatagtgta tataaagatg gtaatttggt
     8041 aaacgaagag atgttttcta attacaatta tgagattaaa gataaagaat tgcttaatac
     8101 agtcaagttt aagtatatta ataaagaaaa aatacagtta aaagtaagtg aaaaaaatta
     8161 tgtaatggag attgtacctt atcaaatttt gacaaataag gtatatgaat ctttaccatg
     8221 tgcagatgga tattttgttc caaataaaga gtattcaaaa ctttgtgttg ttgagagaca
     8281 tagaatgact ggaaatgtag caattgcttc actaaaagga tttggcataa aaaatggagc
     8341 aattgctact actgtggccc atgattctca taatattata gttgcagggg ataatgatga
     8401 tgatattcta gtagctataa attacttaaa ggaaatacaa ggtggttatg taattgtatc
     8461 aaatggaaaa gttcttgcac acctaagtct tcaagtagct ggattaatta gtacttttac
     8521 agctgaagaa gtacaagaaa ttactgataa tatgttggag atagcaagaa aaatgggagt
     8581 tccagagtat gtagaccctt ttattacact ttcttttatg gcacttccag ttgttccaca
     8641 aataagactt acagatttag gtctatttga tgttgaggaa tttaaattta tatagataat
     8701 ataaaagtaa taataaatga ggctttctca aaatagggat aag
//
LOCUS       DACTGW010000161         8672 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.161, whole genome shotgun sequence.
ACCESSION   DACTGW010000161 DACTGW010000000
VERSION     DACTGW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8672
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.161"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            252..635
                     /locus_tag="I9573_002756"
     CDS             252..635
                     /locus_tag="I9573_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797365.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKVVLKSPEYIGSHPKKPGKSVEFIKKIDNYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNNGRVKQYDG"
     gene            1141..1524
                     /locus_tag="I9573_002757"
     CDS             1141..1524
                     /locus_tag="I9573_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAT4797366.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1526..1876
                     /locus_tag="I9573_002758"
     CDS             1526..1876
                     /locus_tag="I9573_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797367.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(1970..2428)
                     /locus_tag="I9573_002759"
     CDS             complement(1970..2428)
                     /locus_tag="I9573_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797368.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2473..3261)
                     /locus_tag="I9573_002760"
     CDS             complement(2473..3261)
                     /locus_tag="I9573_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT4797369.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3400..3837)
                     /locus_tag="I9573_002761"
     CDS             complement(3400..3837)
                     /locus_tag="I9573_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HAT4797370.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            4659..5168
                     /locus_tag="I9573_002762"
     CDS             4659..5168
                     /locus_tag="I9573_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4797371.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVIVIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            complement(6079..6879)
                     /locus_tag="I9573_002763"
     CDS             complement(6079..6879)
                     /locus_tag="I9573_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797372.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(7074..8672)
                     /locus_tag="I9573_002764"
     CDS             complement(7074..8672)
                     /locus_tag="I9573_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAT4797373.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIIW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
BASE COUNT         3141 a         1294 c         1096 g         3141 t
ORIGIN      
        1 tttttataaa gaagcaagaa tactcaataa aacttaaaga aattaaacaa tttctaaaaa
       61 taccttgaaa atagaacctt atttgccatt tataaattat ataaaaaggg attatataaa
      121 gtcatatatg aaaaaactat attatactat cttttctttt tggaataaac tatactttaa
      181 atatagatat ttaatttaaa taaattttac agtattaata gtaaattatc ttagaaagga
      241 atgtgcttta tatgtctaca gacaaagttg ttggaatcat agatgaagaa acggcagaac
      301 ttgcaggaat tgaatatact ggtaaaattt atgcttcttc tggtgttata aaacatataa
      361 agaaaaaaca tagatgtcaa ctgagtaagg atatttttaa tgatataata gacactataa
      421 aagtggtatt aaagtcacct gaatatatag gaagtcatcc taaaaaacct ggaaaaagtg
      481 tggagtttat aaaaaaaatt gataattata ttttagtagc tacagaatta gatacaaaaa
      541 aaggatattt atatgtatct tcaatgtatg ctataaaaga agcaaaatta gaaagtagag
      601 taaacaatgg aagagtaaaa caatatgatg gttaacataa tattattaat tttccccaaa
      661 atattgaaaa atattattat ataagttata atattaatat acacgtagat atttattagt
      721 tagatttgag gttggaaaag gcacccaata cgctactata gagtagttaa tagagatgca
      781 ggatacgccg ccctgccaaa tctaattaaa ttaaaaagag aggattaatt cctctctttt
      841 tgtttttttg agatactaat ttaatctata tagaaagatt gaaggtcatt tatactaaaa
      901 gttttcagtt atatacatta aactaattaa ataagatatt tataatctat atatgataca
      961 tattatatat taataaaaga aaaatatata gaaaatcctt ataaaaattt aaattgtaat
     1021 gaatagtaag taattaaaaa gaatttttat tgatttttaa aaatttagtt agaaactata
     1081 tcattatggt aaagaatgta ttataatgtt tagaaatatt ataataagga gcgattaaac
     1141 atgaatctat cgaagaaaat agaagataaa agaaaagcta aacagagagc tgaaaaagtt
     1201 aagaaagcta aaattgctac agctggtgtg gtattaggag ctgttactgg tgcagtgagt
     1261 ggagtacttt tggctcctaa atctggaaaa gaaacaagag aggatataaa agatgcttca
     1321 caacaaattg ctgaaaagat aaatatgaaa actgtagatg taaaaggcaa agtatctgag
     1381 aagctagagg ataaaaaagg aaactttata gaatcaaaga aaaaaataaa aaaatatata
     1441 gataacaaaa agagtgttga aaattctagt gaggaagata aaataataga tatagttgaa
     1501 ccagttagtg ttactgagga ataatatgga caatacaata acgattaaca taagtataat
     1561 atatgtatta ttagctatac ttgcttgtat tgctttagtt tatttaatta ttgtacttgt
     1621 taaactacat aagattttaa ataatgttgg aactatgtta aatgaaaata ctcaaaatat
     1681 aaatacaact ttaaataatc ttcctaatgt tgtagaaact tttggagaag taggagaaaa
     1741 tatgaaagat gtaacagaag tagttactga agttacagca gattttcttg ttactaaaga
     1801 cagtgtcaaa tcaaatatgg atgttatagt cgatatattg actataatta aaaatatttt
     1861 tctagataaa aagtaataaa tttaatataa aataagatat aatttaaacg accttaaaat
     1921 aacatttata aggtcgtttt ttattagctt gatttgacac tatgtgtttt taataattgg
     1981 taatattcct ctttatcatt tgaacatata tatctatgct tgttgtttca attacatcca
     2041 taagaacatc tttacattca agctcaaaat cttccattcc ttctaaatat tcatcatctt
     2101 catcaaaaaa gatataagaa ttatatagcc attcttcaaa tttttcttca agtatatccc
     2161 aatacgaact ccaaaataat ccacaacctg atatataaga tgctctactt tgatttatat
     2221 aaacctcatt tagaagcttt tgtaatctac tgctataaag aaaatctgct cttccactgc
     2281 ttgatattaa ttcatttaaa tcttctattg acattaattc aactttacca cttttatcat
     2341 aaggagtata attttcatca acccatttat gtggtttgtt ttctctgcat actgttgcta
     2401 tcaactctac aacttttgtt cttatcattt ttttatccta acttatttat tttttataaa
     2461 ataatcatga ttttataatt tatctatttc ttgtaatgct aatttaacag tttcatatct
     2521 atcagcacca tttattacta catcaccttt tacaagttta ttagcttctc cacctacaac
     2581 ataaaccttt ttacctaaac cttgcttata actagacgcc tcaattatta aagaatcttt
     2641 caattgccaa tgtaaaatct gagcagccac tttatcagca tcacctgtat aaacgattac
     2701 attttcatac actttatttt cccctccccc ttgagaattt gatatagatt tgtttagtat
     2761 accttctgct atcaatttag caactttatc tttattatta acataataat ctgtatctgc
     2821 tttactatct acaaaacata cttctattaa tattgctggt gcaatagtat gattaagcca
     2881 ataaagacct ctaacatcag actttgcacc cctatctttg aatactgtag ctaacctagt
     2941 attcactctt tctgcatagg ttttgccatt attagtctta tatattgttt ctgtccctac
     3001 tggagttgaa gttgtagcat ttgaattaaa atgaatctga acagccaaat cagtttcctg
     3061 tttatttgct atagcacatt ggtctgctaa atgattactt gattcatcaa ctcttcctgt
     3121 ataaacagta gctccaccta ttttaagcca tttaacaatt aagtcattca gtattcttgt
     3181 ttctttgctt tcgtttatat acccaacagc tcctgtacct tttccaatta aagtgtgccc
     3241 tggtattatt actactttca aactaacatc tcctttacaa ttaaatttaa cttctattta
     3301 atatatgcat aacctagaat tttgtttctt tttatatctt atacttttat tataacccat
     3361 tttgtaaaaa tatagaatat taagtaaata atgttaaaat tataattttg tatataagac
     3421 attacttgat tgcaataatt ttatagcata ttcatctatt ctatatccag ttctataata
     3481 tatatgttta attccagctt gaattatagc cttagtacaa tttaagcaag gaaagtgagt
     3541 tacataaata gagcaatctt taactgaaat accttctttt gcacaataat aaagggcatt
     3601 ttgttcagcg tgaatggttc taatacaatg tccatctctc atttcatgac caacttcact
     3661 acaatgctta tctccagaaa tactaccatt atatccagta gacactattc tgttttctga
     3721 attgacaatt atagctccta cataagctct atcacaagtt cctctttcag ctactgtttc
     3781 gcacagtctc ataaaatatt cttgccatga gcatctattt tcaagttttt tattcaaatc
     3841 atatacctcc aaaatttata tctaatatat aagatttata ccttaaaaat aatattaaaa
     3901 acaaatttat acgataaata aaatttatta atactgtttt tagtctaaat tatttcattt
     3961 taaattaaag ttacaaggaa tctaaaatta tatatatctt tcttcgtaat atgaatgtta
     4021 caatataaaa aactgtctca ataaaataga gacagtttta cagtcaaagt ttataataca
     4081 agttcatatt tttctttaaa ttcttttaca gcttcgggcc atgccttttg ttctgtaata
     4141 cctttttctt tagctatttt aattgcaatc tctcttatca ttgcagcgcc ttctaatatt
     4201 cccattttta atctcccttc aaatatcttt ttgtatagaa tataaatagt gctaagattt
     4261 attacatgct gcatatacaa tatctataca acacataact tgtaaattcc ttttattttc
     4321 atacgtcttt aaccgacata taattatatt caataagttt taaaattatg aactcaatta
     4381 taattatttt tattttttta gaatctaaac actaaaaatt gtaggtaaat ttgtcttatg
     4441 cctttataaa tatttctaca gttttaatta ctggacatat ctatttttat tataaaaagc
     4501 gcttaaaaat cgagaatatc cgcgtaaaag ctgtatataa gcaaaacgat taaaggaaaa
     4561 agaagaaatt tacttgaatg ttaaaatgcg ttgcttgaag caacgggcag ttttgcctat
     4621 aatatcggta acaactgaaa gaaaggtggt tagccctaat gttaagcgaa aacattaaaa
     4681 caatcaggaa gtcaaaagga ctttcacaac aagaacttgc tatcaaactg aatgtagtgc
     4741 gacaaacaat atctaaatgg gaacaaggac tgtcggttcc cgattctgat atgttgattt
     4801 ccatatcaga ggtgcttgaa acgcctgtaa gcactttact tggagaaact attgttgagt
     4861 cggaagttga tagcttaaaa gtgatttctg aaaaattaga gattataaac ttgcaacttg
     4921 cacaaagaaa aaacatgaga agaaaaatgt ttcattggtt atttatttta ttatgtgcag
     4981 tcatagtaat agtttttgtg gtcttagggg gactaaatag tccttacttg gattggaatt
     5041 ataccgaccc tgaaaccgct gtttttggag tagcttttca tgcttttgag tggctatttg
     5101 tcagattagt accgattgtc cttataggag caattattgg aatctttttg acacgtaaga
     5161 aaatatgaag taactctgct ttttataata tagcaagcgt tccttaaaag tgtcattcta
     5221 catggcaatc gaggacaact atgtaaggaa gaaccttttt gatttccaac taagcgaagt
     5281 tattgaggac gccagtaggc ccaaaaccgc attaagtaaa gtacaggaag caagactgct
     5341 ggacttatac tctcatgcgt cagatgcaga aaatgcgagg tctgtagcat gatttacaac
     5401 gatttgtaca acgactacga gtagaaatat atggagataa gtattatcta cgaaatcaga
     5461 aaagaaatga cttgataatt tactcatgtt ataattattg atgtgaaatt aataaatcga
     5521 aattcctttt ttagatttaa caatactgag taaggaaaaa gtgaatgact tttgcaagga
     5581 ggtgttaata tcaggcaaat aaagtctttt gtttttccag ttatatcctt aatattctta
     5641 gatcaaatta gcaaagttct tataggatta ttcttaatgg actttgaaat tgatataatt
     5701 gggaaatttt taagattcaa tcctgttcaa aatacaaaat taagtctctg ctatctaaca
     5761 aacggagcac aaaaatgaaa attaaatatt aaatataaag cacagttaat tttgattaaa
     5821 aagttgactg tcttttcttt tttataaatt taagcatggg taataaagaa atgactgcat
     5881 ttaagcatta tgtatataat tggaggcaat aaaaagccag caaagcccat aaaatagagc
     5941 tgtgctagca acatgatgag ttataagaag ataatcaaaa gcttacgctt tattttaatt
     6001 taaaaaaatg attactgtaa agaaatcatt ttttattata atcactgata catcaaatat
     6061 tctaaataaa atttttattt atctggaatt gtatattttt tctataatat taactacagc
     6121 gtacattgcc caagcacaaa tagccaatac aaatactccc atcattacta aatctaattt
     6181 aaatacttga gaaccatata ctatcaagta gcctattcca tatcttgaaa ctaaaaattc
     6241 tccaactatt acacctaccc atgccatacc aatattaatt ttagttaaat ttattaaatt
     6301 tcctgtgttt gcaggtaata tgagtttaaa taatatttga gatttagtag ctccaaagct
     6361 ttttaacatc ttaatttttt cttcatctat attcataaaa taattataag ctgaaagtat
     6421 agtaacaaca acagaaatcg ttacagctgt tacgacgatt ccttctattc cagcacctac
     6481 ccaaactata attataggag ctaaagcagt cttaggaaga gcatttaaaa ctaccaaaaa
     6541 tgggtctaat atttttgata atttttctga ccaccaaaga gcaattgcta ccaaaatacc
     6601 taatacagta cctataacaa gaccaagaac agtttcatat acagatattc caatatgttt
     6661 aaataattga ccactaaatg catattgaat aaataaattg taaatatcac ttggtttgct
     6721 aaatagaaat gtatttataa catttaagtc agctaaaagt tcccagatta caataaaacc
     6781 aattaaaatt aatagctggt agaataatac atttcttttt tctcttttta cacctttaac
     6841 atagtttata tatccttcag atcttttatt aagcctcatg tgtatctagc tccttccata
     6901 atatgtcgaa atacttttga aaatcaggaa ctttacgtgt tttgagaggc gtttttgttc
     6961 catccatgtt aagttcaata tcgtaaatat tttttatggt agctggtctt ttagatagta
     7021 ttgcaacctt atctgatatg cttattgctt ctgctatagc taaaaaagtg caactaattg
     7081 cttaaagaaa cagaattatc cacttcagca cccatataaa caatattaag atttcctaaa
     7141 ctgctatccc aaatgatact attaataata gtagataata acaatctctt atcatctaaa
     7201 ctggcattat caacttcatc attaaatctc ttcaaattac taacaataat atcaatatta
     7261 atactgtcac tactaatatt gatattacta gattccaaaa tattaatctt atcctttaaa
     7321 atactaatct cattatttag gctctcaatc tgggaaacta tataatcaga agcagtacta
     7381 ccagtatttt ttgcaagctc tataactaaa tttttaataa gtctttcctt atctaaaatt
     7441 tgatttttaa gtaaattagt ctcagattta aaaatattaa aaacattatc agtcttaatg
     7501 ttattactat tgaatgcact tataatagac tcttctttac aattcttaat tttatctata
     7561 actaaacttt caactaatgg accattaaga tttttacagt tgcaactatc agacccaagt
     7621 gtttttttag taccacaaat ataataatac tttaaatcct tgtcagtttt tttgccttta
     7681 taggtaattc tcatattaga accacattta ccacatttta taagaccaga taaaagagca
     7741 gtttttccag ttgatatcct tggagtttta tcacgatttt tatctaattg attttgtact
     7801 tcaagccata tttcaggttc tatgactcca tggtgttttg caattgctgc tattgagtta
     7861 gatgtatttt tagcataggt aaggattcca cattttctat ctggagtacc tacaacatcc
     7921 ataccactat ttttaaggta atctaaaatt tcatcattag cttttacata tgctggattt
     7981 ctaagtattc tagataaaga actagggtca aatttaccac cacgaggacc ccttattcca
     8041 ttattataga catatttata taacttagat aaggacctaa gttctaaata tttattgtat
     8101 attagcttta caatctccat ttgttcatca tttacttgaa gcttatacat acttctttct
     8161 tttagatttt catctaagaa tgtgattttt tcactgttga atccatgagg tgtattgcct
     8221 ccaagccatc taccactacg ggctaattca tacatgttgt cttttatacg ttctgctatt
     8281 gtttctcttt ctaattgagc aaatacactt gatataaaca tcatagctct tcccattgga
     8341 gttgaagtat caaattgttc tttgatagat ataaatgata tgtttagttc atttaatttt
     8401 tctattaaag tagagaagtc agatacattt ctcgatatcc tatctaatct ataacaaact
     8461 aggtaactaa atttcttatc tttagcatcc tgcatcattt tcttaaattg tggtctatct
     8521 atattgctac cactaaatcc ctcatcttca tatatcaaaa aatcttttac accaatagac
     8581 tttgcataat caatacataa ttgtatttga ttttctatag aatcaccttt tccagtaaat
     8641 cttgattttc ttgaataaat tgcagctttc aa
//
LOCUS       DACTGW010000162         8600 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.162, whole genome shotgun sequence.
ACCESSION   DACTGW010000162 DACTGW010000000
VERSION     DACTGW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8600
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.162"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            230..1309
                     /locus_tag="I9573_002765"
     CDS             230..1309
                     /locus_tag="I9573_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797374.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDEINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYMKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRRH
                     YPGIHSITIIINGVEKGKLDFELGV"
     gene            1633..2001
                     /locus_tag="I9573_002766"
     CDS             1633..2001
                     /locus_tag="I9573_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HAT4797375.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2118..2921
                     /locus_tag="I9573_002767"
     CDS             2118..2921
                     /locus_tag="I9573_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT4797376.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFMPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPSELNQIMDIFIKQLNI"
     gene            3306..4196
                     /locus_tag="I9573_002768"
     CDS             3306..4196
                     /locus_tag="I9573_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT4797377.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSARFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTIQNKDSCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
     gene            4609..5052
                     /locus_tag="I9573_002769"
     CDS             4609..5052
                     /locus_tag="I9573_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4797378.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5945..6853
                     /locus_tag="I9573_002770"
     CDS             5945..6853
                     /locus_tag="I9573_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAT4797379.1"
                     /translation="MTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFINDI
                     LKDAPYANRNIKPFTHIKRIIKAETDPTINYIGEKRPRLYILAEDEESNHINIEMQRA
                     LEDDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLTM
                     DGHPNIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIKR
                     ALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGVEKGKREEKFNIIIKLKNK
                     GYNLSEICDIIDDLNKSEVEKIYNQN"
     gene            7033..7521
                     /locus_tag="I9573_002771"
     CDS             7033..7521
                     /locus_tag="I9573_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAT4797380.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            7607..8014
                     /locus_tag="I9573_002772"
     CDS             7607..8014
                     /locus_tag="I9573_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797381.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            8015..8233
                     /locus_tag="I9573_002773"
     CDS             8015..8233
                     /locus_tag="I9573_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4797382.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
BASE COUNT         3386 a          852 c         1518 g         2844 t
ORIGIN      
        1 agtaaagtat aaaattctat tactataata aaagatagaa ctagaagtct ttatgaaagt
       61 gggaaaagta tttatataaa taaatttata tggtttatag tttaacagta ggaaatatcg
      121 tatgagtgag aatatcgtat tttacataaa gatttagcaa tatgacacac tgttgtcgtg
      181 ttctgtagga tatgattaat aaatcagata tgaaaggaga gtgagtaata tgccagaatt
      241 actaaaaaat atgtataatc gtgagtcact ttatgaagtt gcagtagcta tccaatcagt
      301 ttacaactca tttaaggtag acgagttcat aaaatctaca atggatgaga catggaataa
      361 cttagaatta aaagccagat gtcgaaagat aagtatgagt ttaggaatgt atttaccaga
      421 agactataag gaagctcttt ccattcttga aaaatcagtt acaggttttt attttgcttt
      481 tttctttcca gattttgttg aagtttatgg gcaggacgaa ataaattggg atttatctat
      541 aagtgcatta gaaagaaata ctgagtattg gtcttcagaa tttgctgtaa gagcatttat
      601 catcaaggat gaagagcgta tgatggcaca gatgcgtaaa tggtctaagc ataagagcga
      661 acatgtacga agacttgcaa gtgaaggttg tcgtccccag ttgccttggg gacaagcaat
      721 atccaaattt aaaaaagacc ctacacctgt actgcctata ttggagcaac ttaaaacaga
      781 tacatcaacg tatgtacaaa agagtgttgc taataatcta aatgatatct caaaaacaca
      841 tccagattta gtcataagca ttgcaaagga ttggtacggt aaaaataaat ctactaactg
      901 gattgtaaag catggttgta gaactctact aaaaaaaggc aatcgtgatg tattagccct
      961 ttttggatat gatgatacta cttctataaa tcttcaggac tttactttag aaacaacgtc
     1021 tatttctatt ggagaggatt taactttttc atttaatatt ttagcaaaaa aagcgacaaa
     1081 aacaaggctc gagtacggaa ttgattatat gaaatctaat ggaaaaagga ataggaagat
     1141 atttaaaata tcggaggttt cattaaaaga aaatgagaaa aagtcatata tgaaaaaaca
     1201 ttcatttgca gatgtaagtg taaggagaca ttatccaggt atccactcga ttactattat
     1261 tattaatggg gtagaaaaag gcaaacttga ttttgaatta ggtgtataat tttactaaca
     1321 gcataaccta tatcagatac tttgctaaac ctatatcgta tactctaata aatctatatt
     1381 aggtagttta ctacttgttt actgaattta aatacaaaga aatacaggtg ttaatccctg
     1441 cccattaaag gaatatgagc ataacacaaa agtagaactt atcaattctg ttacgggtat
     1501 agtagtaaat ccatagaatc acaattagaa gatgaagtgt aaaaaataag ttagtgttga
     1561 ttttagttgc tagtgatatt gaatagttat acaatagcca tataaattag aatttgtaga
     1621 ggagaaatgt aaatggatgt atatagtttt tggaatgaca ctttggcaca agatgctgtt
     1681 aagataagaa aatattttca tgaagatgct tatattaatt ggcatgatac aaatgagcat
     1741 tttactttag aagaatttat tattgcaaat tgtgagtatc ctgataaatg gaatggtaaa
     1801 gtagaaaggg tgctagaatt tggaaattta gtaattacag tgacacatgt ttatgcaata
     1861 aataaaccac tatcatttca tgtcgtttca tttatcagcc ttaaagatga taagattatt
     1921 tctattgatg aatattgggg agaggatgat gatgtgccac agtggagatt agataaacat
     1981 attggaaagc caataaaata gtgtttgttc tgttttataa ctgaaatttt gataacattt
     2041 gatattttga tttgtcattg gtcaacatag tcgttagtgt aataatttaa aaaagtagaa
     2101 tttggagagt gagcactatg gaagaaaaat acatcttatc tgaccgaggt aaagtatatt
     2161 attggataaa agaaagtaat agtgtgtttg caaagaactt aatttttttg ccaggactaa
     2221 cagcaaatca tcatcttttt gatttccaga tacagtactt ttcaaaaaat cataatgtat
     2281 tggtatggga cgctcctgct catggaaaat caagacctta cttagatttc tcatattcta
     2341 acttggtaga agaattgaaa gtaattttag ataaacaaaa tatgaaaaag gttgtactta
     2401 tagggcaatc ggcaggggga tttgtggcac agtcctttat tgtaaaatat ccaaatatgg
     2461 tagaaggatt aatgatgatt ggaacttgtc catatggaac tgactattat agtaagtcag
     2521 atattttctg gttaaaacag actgaatgga tgttaaagtt attcccagat aaattgttgc
     2581 gaaacataat atctaagatg tgtggagcta cgtctgaagg cagaaggagt atgatgaaaa
     2641 tgttagaagg ctacgaaaaa aaagaactgt gtcatctcat gtatctggga tttgctgggt
     2701 tcatgccaga aattcaagac ttaaatatac attgtccagt ttgtttggtg gttggagata
     2761 aagacaagac gggtaaagtg agaaaatata atgagcagtg gcacaataag actgggtatc
     2821 cattgcacat tataaaaaat gcttcacata atgtaaatgt cgataaacct agtgaactaa
     2881 atcagattat ggatatattt attaagcaat taaatatata aaaactacat actcattagg
     2941 taaattatgt agtatatgtg tttacatatg gttatagaaa taaatatgta ataaactact
     3001 taacacttaa aaagtggatt tgtccagagc ataatacaaa gcatgatata taaattttta
     3061 agcgaaatac atcaaaagaa gtattagaat tagtggagat aaaacagcta ttagagatta
     3121 taaataaata gttttaaagg tactacgaat atagaggaag gtaaatcata cgttattcaa
     3181 ggattataaa tgagtagttt aaaagtacta tcggataggt ctctgcatat ttaaacctta
     3241 gtaaaggtag ttaaagtagt agtttaaaaa ctaaaaaatt atataaagga attaggtata
     3301 agaaaatgat tgagagtata ccagcaaaac aaattttaca aaaagtgaaa tttgacaata
     3361 caagatggtt tggaattgac tataatatga atctctatcg tggttgttct catggatgta
     3421 tatattgtga tagccgtagc acaatataca acatagagaa ttttgacaaa gttagatata
     3481 aagaaaatgt gatagagatt ttaagtaaag aattgcgttc aaaaagaaaa aaaggggtag
     3541 taggcattgg agctatgtct gacacatata atccttttga aaagcaactt tgtattacaa
     3601 agcaagcact agatttgatt tcggaaaatc attttggagt gtctattgat actaaaagta
     3661 gtttggtggt tagagatata ccgattttgc agaaaattaa aaaaaataat tctgctattg
     3721 taaagttgac tataaccact gcaaatgatg aattatcaaa gaaaatagaa ccttatgtaa
     3781 atccaagttc tgcacgattt gacgcagtga aagaacttaa tgattctgga attttttgtg
     3841 gcatattatt gactcctatg cttccatttt tgactgataa taaagatgaa atacgagcaa
     3901 ttgtagaaaa agcacacaag gctaatgcaa agtttattta ttgcatgtat ggtgtaacaa
     3961 tgcgtagtgg tcagagagag tttttttatg agcatttaag agatatatca ccaaaattag
     4021 tacttaaata tcagaaaaca tatggtttaa actatgtatg tactatacaa aataaagatt
     4081 cttgtgagaa gttgttaaga gaagaatgtg cacgttatgg tattttgaca gatatgaaag
     4141 agattataaa agcgtacaag aggtcggagt catatattca aataaaattt ttttaagata
     4201 ggctttggat gagatataat agtggtgatt gatgtgttga tattatagtt gattgttaga
     4261 gaggatatta tagatgaata ttaatattga tattctaggt aattgctaga actaatatta
     4321 tagatgaata ttagtattga tattctaggt aattgctaga gttagtatta tagatgaata
     4381 ttagtattga tattctaggt aactgctaga gctaatatta tagatgaata ttagtattga
     4441 tattctaggt aattgctaga gctgatatta taatttatta ttaatattaa tattctaggg
     4501 aattatcact atattttaaa aagtaaaaat atatatcaaa atgaactaaa taagtactgt
     4561 aagaagtttg ttaaattaag gtttgaataa agtgagggtg gagaacttat gatagaaata
     4621 aaaactatag aaataaataa aaaaagtcaa attgaccttt taatgttagc cgacccatgt
     4681 gaagaaatga ttgataaata tctcaataaa ggaactatgt acgcattgta tgataataaa
     4741 gaacttactt gtatagcagt tgtaaatgaa atttccaaag aaatatgtga attaaaaaac
     4801 attgctacat atgagcattt tcaaaatatg ggttatgcct caaagatgat ttatcatctt
     4861 ttagatgttt atagtaaaaa atattcatca atggttgttg gtacatctga aagcggagtt
     4921 cctttttatg agaagtttgg atttgtttat tcacataaaa ttaaaaactt ttttgtagat
     4981 aattatccag aaccaatctt tgaaggagaa ttacaatgtg ttgacatgtt gtatctatca
     5041 tataactttt agataggata atatttttag gtagatataa ttaattaatt catataatta
     5101 tcggaattat aatttataaa ttatgtataa taagagtata atttatttag aagctgttat
     5161 attgtattat attcaaataa gatatataat aatttgttta ttttgaattt tggaggatta
     5221 attatggatt caaataataa tactataaaa tcaactgtta aaaagggtat ttcttttggt
     5281 tcttgtttag caatgattat ttcttatact gcatggaaat ctattccatg ggctattttt
     5341 catggcttaa tgagttggat atatgtactt tattattggg ttaagtatgc atagattaaa
     5401 ttaataaata agtatgtaat attgtaaagt ttaagaaaca aaatgtaagg gagtgtctcg
     5461 aagtgaactt tttagtttat aaggcactct tttttgtatt aaataaaata tattttcatt
     5521 acttaaacaa tgaaaaagat gagtatctct attttaatac atacccttat attcattcat
     5581 ttatcaacaa tagttttaaa aagaaagcta tctaaaatct attgtaatct aagtgcttct
     5641 ctttaaagtc ttaactttag taatgatata taagtttata aggttcttat ttcaataatg
     5701 caaattttac ttagaagttc agactttggt atgaagaaat gggtaataat aaaaaagagg
     5761 tgttgatatg aaagaaaaat tagaagaaaa atctaagatt attaaaacat caaaagagta
     5821 taaacgaact ataattgaag aaaaggacag agaatgaacc aaaaaattaa atgacatact
     5881 tagtagaggt gatttttcta atgaaaaatt agataaaatc aaggatttaa ttccaaaaga
     5941 aatattgact agtgaaaatg ttggagaagt tgtaactgaa gatgggtatg ctaagcctga
     6001 gtatgatgag gtatttaaat cattgtttac tttaaataat gattacgatt tacttgcaaa
     6061 ttttataaat gatatcctca aagatgctcc atatgccaat aggaatatca aaccgttcac
     6121 tcatataaaa agaataatta aggctgaaac cgacccaaca attaattaca ttggtgagaa
     6181 aagacctaga ctttatattt tggctgaaga tgaagaaagt aatcatataa atattgagat
     6241 gcaaagagct cttgaagatg attatttgga aagagctgaa tattatctta gtcgtgtaca
     6301 tggtagaaag ttagaagaag gtaaagagta taaagaaatt ggaaaaactg ttggaataca
     6361 tatactaaat catgtaaaat ataatcacat agaagattat gtaaattgcc tgagattaac
     6421 aatggacggg catccaaata tttattctag taaaactgca ttatatttta tagaacttcc
     6481 aaaaattcgt aaatccagtt gtatagcaaa cagagttcta atatggggta aacttattga
     6541 taatccttct catattgata ttagaatact atctaaaaca gattatgtta ttaaaagagc
     6601 acttgataga ttaaaagaat taggcagtaa tgaggactat ttattaaatt taaagagagg
     6661 tgcgtatatt atgaataaaa gtagaaattt taaagaagaa atttttcaag aaggtgttga
     6721 aaaagggaaa agagaagaga aatttaatat catcataaaa ttaaaaaata aaggatataa
     6781 tttgagtgaa atatgtgata ttatagatga tttgaataaa tcagaggttg aaaaaatcta
     6841 taatcaaaac taatactata attgagatgg cactagataa atataaagct tcctctttta
     6901 aatttaaatt aatataaaac cttgttattt aactatagta gatacaaaga gatatataag
     6961 ctaaggagat gattcaaaat aaaagtttga actatctcct tcttatagaa aaacaaaggg
     7021 gttgatttat atatgaaatt ttatactata cagactttag aattttggaa caacaataaa
     7081 aacaataaat atctatcaaa tgattattct tttatattag aagactttga tattccatac
     7141 aaatggatgt atagtcaaat gacaaataga atatatgaat ttgatgatag tatgatatgg
     7201 gtttggacga agagacctga tttaagatgt agtggttatt tagaaaaagg aaaaaaaggt
     7261 gttttacttg aaatagaaat aagtgaagac aatgtgttat tatcagaatt tgatttatgg
     7321 catttacctt taatggatat tactgtagag ctttatgaag atgaacaaat tgaaaagaag
     7381 gtaagttggg aaagaatatt tgacttagaa ctgtgttcaa aattaaatga agttaatata
     7441 gaagatttga cattgcaagg agtgacatct aaagtgcctc tatctaatgt aaaattaata
     7501 aaagaatttg ttgcaaaata atatttttat tgaaatttat tcaaaaaagt tatatattta
     7561 taacaagagg tgttataaat acataacttt ttaaggggga ttttatatgt ctaaacaaca
     7621 aactggtatt aaatcaacac ttatttcaga aatattggga atttcttatt gtattggagc
     7681 tacattagta aataagtatt tttttaaagg aagtagtatt gaatatcttc ctataattat
     7741 ttgggtatta gctatgggag ctcagtgggt ctataataaa aagtatgatg atttaattga
     7801 tgaggcatca aaatgtatac tatcaagagt taatgatatt gcaataaaag tattgttttt
     7861 ttctgtagct atagtatcta tatttcttgt aactttggac ataaatgtgt caaatttaaa
     7921 tataggaatg tctttattag tcattttgtt tattcaatcc ttgttgaagt taattctatt
     7981 tatatatttt gacagaaagg ggatatacaa ttaggtgtca gtattactaa agacaaaaat
     8041 taaatcctat agagaaaaga atcttatgac acaaggtgaa cttgcaaagt cagtaggagt
     8101 aagacgtgaa actattgtac atttagaaaa tggtcgctac aatccttctt taaaactggc
     8161 aatggatatt gctaaggtat ttgatacaac tgtagaagag ttgtttgaat ttattgaaga
     8221 aaataatatt taaatttcat gtattactta tgcattagag aatgaattaa tagataaaaa
     8281 attcaagttt atgtattaga gaatgaatta attataggta aaaaaccaag cttatagatt
     8341 agagaataaa tgacttatag atgaaaaacc aagctcatag attaaagaat gaatgattta
     8401 tagatgaaaa agaaagctta tagattagag aatgaattaa ttataggtaa aaaacaagct
     8461 aatagattaa aaaatgaatg atttatagat taagaagtaa gcttatagat tagagaataa
     8521 atgacttata aataaaaaga aagcttatag attagagaat gaattaatta taggtaaaaa
     8581 acaagcttat agattagaga
//
LOCUS       DACTGW010000163         8597 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.163, whole genome shotgun sequence.
ACCESSION   DACTGW010000163 DACTGW010000000
VERSION     DACTGW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.163"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(91..945)
                     /locus_tag="I9573_002774"
     CDS             complement(91..945)
                     /locus_tag="I9573_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HAT4797383.1"
                     /translation="MKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKEVP
                     SKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDIEE
                     VEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCDKI
                     IKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLNTI
                     KEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEEEE
                     EKYRRIFK"
     gene            complement(1268..2596)
                     /locus_tag="I9573_002775"
     CDS             complement(1268..2596)
                     /locus_tag="I9573_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HAT4797384.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2744..3073)
                     /locus_tag="I9573_002776"
     CDS             complement(2744..3073)
                     /locus_tag="I9573_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAT4797385.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3097..4386)
                     /locus_tag="I9573_002777"
     CDS             complement(3097..4386)
                     /locus_tag="I9573_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAT4797386.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4402..4728)
                     /locus_tag="I9573_002778"
     CDS             complement(4402..4728)
                     /locus_tag="I9573_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAT4797387.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4863..5492)
                     /locus_tag="I9573_002779"
     CDS             complement(4863..5492)
                     /locus_tag="I9573_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797388.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5707..6723)
                     /locus_tag="I9573_002780"
     CDS             complement(5707..6723)
                     /locus_tag="I9573_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAT4797389.1"
                     /translation="MVKAVGDIIGKCSAISFDMKLDKSYDEVIVDFKNLINNIDNKDV
                     LLFTDMGSLNSFDEIIKKEKKCEVRVIPMVTTLTVLEAVQKANMGLPLNDVYNSITNT
                     RKYYFGTNEIQNKENLSKTIIIASHVSEGVDNKTRKILEEKMSRYLDGIDIISVPYKT
                     EKDLSLNITKLKESSNIVAVINEQRINIRGIDYISKKDIDKDENINKLKNIIKISIGY
                     DDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLVFNIEKKYDKVIGLMMHLAFMVDGLI
                     GNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDKKYNIEINEKECYQILLILEYAEIIE
                     KDYQ"
     gene            complement(6704..8362)
                     /locus_tag="I9573_002781"
     CDS             complement(6704..8362)
                     /locus_tag="I9573_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4797390.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERDSKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASNGKSSW"
BASE COUNT         2626 a         1526 c          879 g         3566 t
ORIGIN      
        1 ttttattaat ttttactact tgtaaattat tatcttatac aatgaatttg tattatataa
       61 taagtatttt taggattctg ttacataata ttatttaaat attcttctat atttctcttc
      121 ttcttcttca gtaaacacat taaatacaac ttttaaattt ttttcaggat tttcttttag
      181 ccatagtcta actctactta cagcaagatt agctgcctct ttttttggat aaccaaatac
      241 acctgtagaa attccacaaa aaacaatatt ttttatttca tcaatttctt ttattgtatt
      301 taaacatgat ttataacaat gaagcagttg cttttcttgc tctttacttg gctgaccacc
      361 agatacaata ggtccaacag tatgtactac aaattttgca ggtaaacaat agcctctagt
      421 tatcttagca tctccagtat attctaaatg cccttgtttt tttattattt tgtcacaatc
      481 ttctctaagt ctaggacctg cgcatgaatg tatctcatta tccacgcata gatgtaatgg
      541 ttgtaaacac cctaataact tgttatttgc agcatttact attgcatctg ctcttaaatt
      601 cgttatatta cctctccata tggcaatccc ttcctctact tcttctatat catttacatc
      661 tactaactct ctttcttcac attcaatcaa caataaatta tgtaacatat taagtatctc
      721 tggagcaatt tcttttggtg gatatacatt caaaagtcct ctaagtatat ctctaggctt
      781 aatttcatca tttgataaat tgtatatttt agaaggaact tcttttgaaa aatatgcaat
      841 taaagtgttt atattttttc ttctttgttc gtcagttaga ggcttgatag ttttatcaaa
      901 atcctcaaat aaatttacat catcagcata atctctccac ttcatagcca tctctccatt
      961 cttgtatttt agacttaaaa ataattatgt atttatgtac atatttattt atttctataa
     1021 acacctacat aaatcctgtt aaaagtgtaa attttttatt atgcaacttc tctggttatt
     1081 tctattgatt gatagtaaat tattgtacct aaatttaatc caactaacaa gtaactaaac
     1141 ctactttcct aactgcaaaa tacactatgt ttgccacttg tatctcattt gtaaataaat
     1201 aaagtcccta caaatacatt aactaggcta tgtactaaac tgtaggaact ttaaaaatat
     1261 attattctta attaagtttt ggccaataat ttttatttgc ttctattaaa tcatctagta
     1321 attcttttgc aactgttgca ctaggaactg tcttagatag cataagtgct tgccacaatt
     1381 tattataaga cttctcaatc caagcatcaa ctactaattt ttcaactgct aattgttgtt
     1441 ctattaaacc tttttggaat gttggtattt tccctattga taatggttca tacccgtctt
     1501 ttccaactat acatggaatt tcaaccatag catcatcttg tatattttct atagctccat
     1561 tattttgaac tatcaataac atacgctcct gtgtattaaa agctagagct gtagctaaat
     1621 ccactataaa ctctgcatgt attcctatct taagcccact gcccttagaa tgtccttgct
     1681 tttctatcaa tgcacatgaa ccaaaaactt ctttttctct tccatcaata acttcattgg
     1741 cacgagtata atttacatca gaatgattta caacataatc tggaaataga tagtacttta
     1801 agtaactact tggtattgta gttaaatctg tttttactat ttctttagta aataaattag
     1861 tttcaaacca actatctttc ttgtcatttt tcattccttc ttcaccattt ataaaaccat
     1921 attcttttat atgttctact aattttggta aaagttcatt tcctttttta tctttaattg
     1981 aagtatacca accaaagtga tttaatccaa agtatcttat atccatatgt ttacgagatt
     2041 tgagtcctgc aatccttgct atattatgtt ccatacctat tggcatatca caaatattta
     2101 ttaccctaga attaggtctc aaccttctaa cagcttccgc tattattgat gctggattag
     2161 aataatttaa catccaacaa tttggagaat acttttccat ataatctatg ttttcaagaa
     2221 caccttctat agacctcatt ccataagcta ttccacctgg tccacaagtt tcttgaccaa
     2281 ctactccatg ttttagaggt atcttttcat ctagttctct catttcaagt tttccaactc
     2341 ttatatgagc taaacaaaaa tctacatctg tataagcctc tttagggcat gttgttgcta
     2401 aatactctat attagggtcc ttttccttta ttagtatttc acaagcttta gctaccttat
     2461 tttgtctttc ttcatcatta tcatataatt tgattgctct taatggtaac ctatctaagt
     2521 tatctagcaa cattaatatg atttctgggg tatatgtact tcctcctcca gctattacta
     2581 ctgaaaattt ttccataata atatctcctt ttctatttaa aaatttactg tatttataaa
     2641 taaatttata aatactagta ctttaaagta aaagggagtt catttattaa atctattgat
     2701 ttaataaact atagccaaac ttcaagcact atttacaaga aatttaattc ttattaattc
     2761 ttgggtccat catctcaaat atatctacaa gttcagatac taaagaatga gcaaataatg
     2821 catttgaata atggtcttca gagtgcacaa ctaaaaaatc agtcccacta tcttcactat
     2881 tcatagcatt tgatagtaat tctaaatgat gctttgatgc tgtttgtatt ttctctgagg
     2941 ctgaatttaa aaggtgtttt gctttattgt agtcaccttt ttttatttcc ttaagagaca
     3001 tcattacatc acttttagca tctcctgctt ctgatattat actaaatact ttttcttcca
     3061 tttcaggtac catatccttc tccttctaat gacttgttaa aaatcaaagc tatcatcatc
     3121 taatgttgta gctaattctg catttttatt taattcttcc ttctccatta tcttaaagaa
     3181 tggataatat atcaaggtca taagtattat acttgcaaat actaatacga ttgccttcca
     3241 atctaatgtc atcaaaaatg ctgcaacccc tggaggtaaa aagcctggtg gttctgcaat
     3301 aggtaatcct actaatccta cttttaatgc cataaaatta agtgctccaa ccaatacagg
     3361 tccaaataca aatggtacaa acatatatgg gttcataact attggtgttc caaatattac
     3421 tggctcattt attccaaata gtgatggtaa aatcacaact tttcccatac tattaaaact
     3481 cttacactta cttcttgaca taaggtacac taatcccaaa gttatccctg aaccagttac
     3541 ttgcaagaaa ccatagtaaa atcctattgt aaacacatat tttgggtctc ctcctgctgc
     3601 tatcagtgtt gcattttctg ccatatatgt agctgctatt ggcatccata tacttgaagt
     3661 tattgctgct ccatgaaggc caaagaacca taataattga gttataaata gtattatagt
     3721 tactgcaaaa acattatcta cactgcttat agctggtgct aagaaattca ttataatagc
     3781 tggtaatggt tgacctgaga catttttagt tattgctgct attgctgtaa ataacattat
     3841 acatactatc attggagtta aatttctaaa acttttattt actacactag gtacttcttt
     3901 tggaaatcta attacgagat ttttatcatt cataaactta gtaatatcaa ctactaaaag
     3961 tgctactaat atacttacaa ataaaccttg tgcatctaaa taacttgtat ctaatacacc
     4021 atctactact tgtccacata caacaagttg tgcaacaact gctgtcatac ctatttctac
     4081 atgattaaga ttatattttt tagctagtgc ctgagctata aaaaatgctg aatatataga
     4141 taataaactc atagtaaatt tattagggat aagtaatgta tccatatttt cagataataa
     4201 tgtttttgca cttccttcag gtaacatatt gtttaatgcc ataaataaca tacttaaaga
     4261 accaacaatt attattgggg taatgcttat cataccttct tttatagctt gaagatgcct
     4321 ttgtttttca atcttttgtg ctattggtag aagaaatctt cttaaattat tttgaagtgc
     4381 ttccattttt attcccccca attatctatt tttatataat tcgattgccg acttcataac
     4441 agaaggagca tccatacgac cataatgctt agtatctatt aaaccaactg ctattttgta
     4501 attcttacaa gcttcaacta catcatcttc cttgaaccta atttgtggtg ctacaagtac
     4561 tacatctgta tcattagctt caatgtatga ttttaaattt tcgaatgatg ttgcctctat
     4621 atttatagtt ttagttttta acttttcatg ctcactacaa aaacttttca tcttttccac
     4681 aagtacacct gttgaagctc catttgcaca tactaataaa atattcataa ttaccctcct
     4741 acaatatcta acctctttta ttttctaatt ttttatcagc caattcttac tctaattcct
     4801 atcagttttt actttaattc ttattatttt attttaattc ttattaatat ttactctaat
     4861 tcttagtact gccaatgatt aaacttgaaa gccgtatcta taacttcttc ttgcttatat
     4921 ttttctaata tttttaagta tctttcttta taatcattat ctacttcttt ttctggtatt
     4981 actatactag cttcatttaa aaagtggaat gaatcccttc ccatttctaa tcctctatat
     5041 gtatgcttgt caagtgccac gtccataact tctggaactt tacccattgc aaatcctttt
     5101 ataattatat tttttaataa atcacttgat ctatctttct cacatgcaca aagatatctt
     5161 attgcatgta tgaaaaataa aggtctgtct ccatctgcat atgaatattc ctttcttact
     5221 tgatttaaat tatttatgat tattacagct gatgggtccc ccattcctat atcttcaact
     5281 gatattgtaa gtatccttct ccatagctta tcttctaatt ctggtgatga tatatatagt
     5341 tcataagcaa attcacaagc ttcttccacc atatttcttc ttatagcttt ttgaaatgac
     5401 gatattattt cgtctgcact aaatccgttt cttgtagtca ttcttgccca tgggtcattt
     5461 gcctttttat ctcttaatat tttatcttgc atattattta ctccttttta tttaaatttt
     5521 aattatttat attactcatt taataatata agcaagtatt atgccagttt ttttagacac
     5581 tttaaaacac tgttatttca atcattttta tcatcaaaac ttagatactt tgtctaagta
     5641 tatttatatg tgtctaatat taatattatt ttagactaaa aaaaattacc aaataagttt
     5701 ttatactcat tggtaatctt tttcgattat ttctgcatat tctaatatta ataatatttg
     5761 atagcactct ttttcattaa tttctatatt atatttttta tctaattgag atactatatc
     5821 ttttattttg cttaggctct tgtgataatc aagtgttttt tctttatcaa gcttttcaat
     5881 ttctctagta tttccaatca acccatctac catgaaagct aaatgcatca taagtccaat
     5941 tactttatcg tatttctttt ctatattaaa tactaaaaac aattcatcag acacatattt
     6001 tatatcttta aatatcctat tataattaga gctttttaag gatgtcttta aaccttcaac
     6061 tacatcatca taacctatac ttatctttat aatatttttt aatttattga tattttcatc
     6121 cttatctata tcttttttgc taatatagtc aattcctcta atattaattc tttgttcatt
     6181 gattactgca acaatattac tactttcttt aagttttgta atatttaaac ttaaatcttt
     6241 ttctgtttta tatgggacag atataatatc tattccatct aagtacctac tcatcttttc
     6301 ttctaatatt tttcttgttt tattatctac accttctgaa acatgtgatg ctataataat
     6361 tgttttagac agattttctt tattttgaat ttcattagtt ccaaaataat actttctcgt
     6421 gttagtgata gagttgtata cgtcatttaa aggtaatccc atattggcct tctgaactgc
     6481 ttctaacaca gttaatgtcg tcaccatagg aattactctt acttcacact ttttttcttt
     6541 ctttataatt tcatcaaagg agtttaatga acccatatct gtaaatagta atacatcttt
     6601 attgtctata ttattaatta aatttttaaa atctacaata acttcatcat agcttttatc
     6661 taacttcata tcgaaagaaa tagcactgca ctttccaata atatcaccaa ctgcttttac
     6721 cattacttgc tgtagtttct ccatgcatag caactataat attaatgtta ttcactattt
     6781 tattttgttc atcttgttta gcttgtacta tgaataatgt aatatatgct acagtttcta
     6841 tagataaaaa taaatcaaat tcattttcaa ttattccaac tatttttagt gctgttttaa
     6901 attcacttct gttatttctt ctaatttgat tcaaatctat gttgtttgta gagctacttt
     6961 tatccaatac gctttttata taaattgcca ttgacttaaa tattgattca cttatatcta
     7021 tttcttcatc ttctttaata atttgtctta ttgattcaca catgctttta aacttaacat
     7081 tattaaatag agaagcatct gctatattca tttctacaga tttattaaga ttagtattta
     7141 ctgcattaat aatatttata tcattgtagt taataacttc ttcttctcct aatgacgtaa
     7201 atacaaaata ttctattcca tgtgttataa cttcatctgc tttaatagac ttctctctat
     7261 aatcatcact cacttcattt atagatttat caaaatattt tttctctata taaactggtt
     7321 ctttattatc tcttttatat cttaggtatc catttgcaac agctaatttt atatcatttt
     7381 gaagttgacc tacattattt ttacaatcat aaagtaatag tgatttaatt atttctttat
     7441 gtacttttat tgaaatagaa atactttttg attcttcctt gaaaaaattt ttgactaatt
     7501 ccattctttc aatcattgtc ctatctctca gtgatggtat ttctattttc attggtattc
     7561 ttctattgaa tgtatctaat agtgttgatg tcggattttc tgtagtagca catattatta
     7621 ataccttaga atctctttct ttcgttgcat ctcctagtct tttaaatgtt cccttatcta
     7681 taaaagtaaa caacatttct tgaccttctg ctggtagtct atgaatttca tctaaaaata
     7741 gtatccctcc atttgcaatt tccaaaagac cttttctgga agattctgca cctgtataag
     7801 ctccttgttc tactccaaaa atatgtccca taagtaattg tgcattatta gcataatcag
     7861 cacaattaaa tgttacaaaa ggagctttat tttttctaat tccactatct atagaataat
     7921 cgtacatata tctagcaatc atactctttc ctactcctgt ttctccatag ataatagtat
     7981 gaagtccatt tgtaggatag agcattgcga ccttgcattt ttctattata ggtcttaaac
     8041 tatgttcttt acctattagt gtttcaaaac tatctttttt tattttgttt tctacttttg
     8101 ttcctttttt tatgccaagc aactcacttt gtgtatcaag caattcattc ttgttaaggt
     8161 taagatttgc agggatataa tacacaggtc ttccttttat cttcgtaacc ataccctcat
     8221 tgaataattg gtttaaatat gtacttatat tgtttcttgc aattcctgtc ttttctgata
     8281 attggttagc tgtaatccct ttttcatttt ccctatatga tgattctaaa actttcatta
     8341 ctttatcttt tttatatatc attacaagct cccctacatt ataatatttt attaactaaa
     8401 acccctatac acactttatt ttttaataaa ctcttattct ttaactaagg ttataattta
     8461 tcaaactttc cttactttaa attatagaaa attattgtag aaaaaataag attattgctc
     8521 attttttcta cattactatt tttaatttta tagcactttc ttaacaacat actttacttg
     8581 tttttaattt tattata
//
LOCUS       DACTGW010000164         8457 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.164, whole genome shotgun sequence.
ACCESSION   DACTGW010000164 DACTGW010000000
VERSION     DACTGW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.164"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1..183)
                     /locus_tag="I9573_002782"
     CDS             complement(1..183)
                     /locus_tag="I9573_002782"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797391.1"
                     /translation="MRDEGQNDRENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            complement(272..433)
                     /locus_tag="I9573_002783"
     CDS             complement(272..433)
                     /locus_tag="I9573_002783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797392.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYMDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            complement(512..1012)
                     /gene="tcdE"
                     /locus_tag="I9573_002784"
     CDS             complement(512..1012)
                     /gene="tcdE"
                     /locus_tag="I9573_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HAT4797393.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAVLLDAMTDEM
                     NAKDEK"
     gene            complement(1134..8234)
                     /gene="tcdB"
                     /locus_tag="I9573_002785"
                     /gene_synonym="toxB"
     CDS             complement(1134..8234)
                     /gene="tcdB"
                     /locus_tag="I9573_002785"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HAT4797394.1"
                     /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIVESATNDTLESFREN
                     LNDPRFDYNKFYRKRMEIIYDKQKNFINYYKTQREENPDLIIDDIVKIYLSNEYSKDI
                     DELNSYIEESLNKVTENSGNDVRNFEEFKGGESFKLYEQELVERWNLAAASDILRISA
                     LKEVGGVYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMVKLEAIMKYKEYIPGYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNAFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKKNYFEGSLGEDDNLDFSQNTVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGLDVDSLSTEIETAIDLA
                     KEDISPKSIEINLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKIIVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLAECEINVISNIDTQVVEGRIEEAKSLTSDSINYIK
                     NEFKLIESISDALYDLKQQNELEESHFISFEDILETDEGFSIRFIDKETGESIFVETE
                     KAIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDATHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     VIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTAATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELILRDKATKVVDYFSHISLAESEGAFTSLDDKIMMP
                     QDDLVISEIDFNNNSITLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPVITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMNINIELNENDTWVIDVDNVVRDVTIESDKIKKGDLIENILSKLSIE
                     DNKIILDNHEINFSGTLNGGNGFVSLTFSILEGINAVIEVDLLSKSYKVLISGELKTL
                     MANSNSVQQKIDYIGLNSELQKNIPYSFMDDKGKENGFINCSTKEGLFVSELSDVVLI
                     SKVYMDNSKPLFGYCSNDLKDVKVITKDDVIILTGYYLKDDIKISLSFTIQDENTIKL
                     NGVYLDENGVAEILKFMNKKGSTNTSDSLMSFLESMNIKSIFINSLQSNTKLILDTNF
                     IISGTTSIGQFEFICDKDNNIQPYFIKFNTLETKYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVIISPNIYTDEINITPIYEANNTYPEVIVLDTNYIS
                     EKINININDLSIRYVWSNDGSDFILMSTDEENKVSQVKIRFTNVFKGNTISDKISFNF
                     SDKQDVSINKVISTFTPSYYVEGLLNYDLGLISLYNEKFYINNFGMMVSGLVYINDSL
                     YYFKPPIKNLITGFTTIGDDKYYFNPDNGGAASVGETIIDGKNYYFSQNGVLQTGVFS
                     TEDGFKYFAPADTLDENLEGEAIDFTGKLTIDENVYYFGDNYRAAIEWQTLDDEVYYF
                     STDTGRAFKGLNQIGDDKFYFNSDGIMQKGFVNINDKTFYFDDSGVMKSGYTEIDGKY
                     FYFAENGEMQIGVFNTADGFKYFAHHDEDLGNEEGEALSYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGISIINDGKYYFNDSGIMQIGFVTINNEVFYFS
                     DSGIVESGMQNIDDNYFYIDENGLVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFDPETKKAYKGINVIDDIKYYFDEN
                     GIMRTGLITFEDNHYYFNEDGIMQYGYLNIEDKTFYFSEDGIMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
BASE COUNT         2780 a         1424 c          872 g         3381 t
ORIGIN      
        1 ttagttaatt gactcttcta ttagataagg agattgtctg atagtaattt gtaaacatat
       61 cttatatttt tttaatgttt ttatttcttt atatattttt cctataactt ttaaactatc
      121 aattaaatca tttattaagg tttgatactt attttctcta tcattttgtc cttcatcacg
      181 tatttttgtg tttttgaata atttccatca caatttacac atatttctat gtttaactat
      241 tatagtttgt gatatctgta actaatgtag cttaatagat tttaccaact gaccatgcac
      301 catctttgtg aaccaatgtt tgatatacta ccttgtcatc catatagtaa tgaacacttg
      361 cttcgaaatt atatgtattt aatacttgtg tgtctcttgg catctgctcc ctcgttttca
      421 ttatcagttt catgaattat gataactttc agctattctt atttggataa cactttttat
      481 ttgttaatat ttttattata tctaccatta cttacttttc atccttagca ttcatttcat
      541 ctgtcattgc atctagtaaa actgctattt tttcctttaa tctcttaggt actggtaatc
      601 cacataagca catatttttt aatatgctta cactttcata taaaataaat aaaatagaga
      661 aaaattcaga tattccaaga tgttttagtc ttaaaaaatt gatacaatct tgtggtaaca
      721 taaataaaaa gttaaacttt gtaagaatgt caactactga taaaaaaaat atacatgcta
      781 tcattgctac ttttctgatt cctccatcta ttccaaaact agaattaaat ttacgacttt
      841 ttattgcact taaacatcct aataatgtat caaatgatat agttaaaatt actaacttta
      901 taaatatatg ctctgataaa aaagatattg tcatattcat aacgcctcct aggtttatat
      961 aaaaaaatat tttgttacca ttagaaatat aaaaaggtga actactgtgc attcatcata
     1021 gttcaccttt ttattggtaa atcttcatat tagatagaac actttatgta gtgtatcatt
     1081 ttgtttattt ttatatattt aagtataaga ggatatattt aacgtatttt tatctattca
     1141 ctaatcacta attgagctgt atcaggatca aaataatact cctcaccatc aataataact
     1201 gaaccagttg ctgcaatata ttcatctgta aaataatatc tcttttcatc taaatctaac
     1261 caaccagtat agtttattga ttctccctca aaattctcat ctaaagtatt ttgatgtgca
     1321 aaatatttaa atccatctgg tgtattaaat actccaatct gcataatacc atcttcacta
     1381 aagtagaacg tcttatcttc tatatttaga taaccatatt gcataatacc atcttcatta
     1441 aaatagtaat gattatcttc aaatgttata agacctgttc tcattattcc attctcatca
     1501 aaatagtatt ttatatcatc aattacatta atacctttat atgctttttt agtttctgga
     1561 tcgaaataat atttatcact ttcattttcc atatcatata tccaaccagt ctcaattgta
     1621 tatgtttctc caaaataata tacatcttca ccaactctaa ctaaaccact atattcaact
     1681 gcttgtccat agatattatc atttacagta ttagctggtg caaagtattt atatccatct
     1741 gaagtgtcaa atacaccaat ttgaactaga ccattttcgt ctatatagaa ataattatca
     1801 tctatatttt gcattccaga ttctactatt ccagaatcag agaaataaaa tacttcatta
     1861 tttattgtga caaatccaat ttgcatgatt ccagaatcat taaaataata tttaccatca
     1921 ttaattattg agatacctat atatgcttct gctgtatctt catcaaagta atattttgaa
     1981 ccatcttcta aatccttcca tccaactact gctgtaaatg aatcatcaaa ataataaatc
     2041 ttattgttaa aattaagtat accagaatat gaaagtgctt caccttcttc atttcctaaa
     2101 tcttcatcat gatgagcaaa atatttaaat ccatctgctg tattaaatac tcctatttgc
     2161 atttctccat tctcagcaaa gtaaaaatat tttccatcta tttcagtata tcctgacttc
     2221 atcacaccag aatcatcaaa atagaatgtc ttatcattta tattaacaaa tcctttttgc
     2281 ataataccat cagagttgaa atagaattta tcatcaccta tttgatttag ccctttaaaa
     2341 gctctacctg tatctgtact aaaatagtac acttcatcat ctaatgtttg ccattctata
     2401 gctgctctat aattatctcc aaaataataa acattttcat caatagttag tttgccagta
     2461 aaatcaattg cttccccctc tagattttca tcaagtgtat ctgctggagc aaaatattta
     2521 aatccatctt ctgtactaaa tacacctgtt tgtaacactc cattttggct gaagtagtag
     2581 tttttgccat caattattgt ttctccgact gaagcagctc caccattatc tggattaaag
     2641 tagtatttat catcacctat agttgtaaat ccagttatca agttctttat tggtggcttg
     2701 aaataatata atgaatcatt aatatatact aatccagaca ccatcattcc aaagttatta
     2761 atataaaatt tctcattgta taaagaaatc agacctaaat cataattaag taatccttcc
     2821 acataatatg aaggtgtaaa tgttgaaata actttattta tagatacatc ttgcttatca
     2881 ctaaaattaa aagatatctt atctgatata gtattacctt taaaaacatt agtaaatctt
     2941 attttaactt gtgatacctt gttctcttca tcagttgaca taagaataaa atcacttcca
     3001 tcattactcc atacatatcg tatagataaa tcattgatat taatattgat tttttcactt
     3061 atataatttg tatctaatac aatcacttct ggataagtat tatttgcttc atatataggt
     3121 gttatgttta tttcatctgt atatatattt ggcgaaatta taactttatt aacacaactg
     3181 tctattccat aaaggtattt ctgagaaaaa ttaatgacag ttgaagatat atctccagaa
     3241 tcatctaaat cataatttgg ttctactatc atattttgtc tattacctac atatagagta
     3301 tatttagttt ctagtgtatt aaacttaatg aaatatggtt gtatattatt atctttatca
     3361 caaataaact caaattgacc aatagaagta gtaccactta ttataaaatt agtatctaat
     3421 ataagcttag tattagattg taaggaattt atgaaaatac tttttatatt catactttct
     3481 aaaaagctca ttaaagaatc tgaagtattt gtactacctt ttttattcat aaatttcaat
     3541 atttcagcta ctccattttc atctaaatat actccattta attttatagt attttcatct
     3601 tgtatagtaa aagaaagaga gatttttata tcatctttta aataatatcc tgttaatata
     3661 ataacgtcat ctttagttat gactttaaca tctttcaaat cattactaca atatccaaat
     3721 agaggtttac tattgtccat ataaacttta cttataagaa ctacatcaga taattcagat
     3781 acaaataaac cttcttttgt agaacaatta ataaatccat tttcttttcc tttatcatcc
     3841 ataaaactat aaggtatatt tttttgtaat tcgctgttca atcctatata atctattttc
     3901 tgttgaacag aatttgaatt tgccatcaat gtttttagtt caccagaaat aagaacttta
     3961 tatgatttag ataataaatc aacttctata actgcattta ttccttctaa gattgagaat
     4021 gttaaagata caaatccatt acctccattt aatgttccag agaaattaat ttcatgatta
     4081 tctaaaataa ttttattgtc ttcaatactt aatttagata aaatattctc tattaaatct
     4141 ccttttttaa ttttatcaga ttctatagtg acatctctta cgacattgtc gacatctata
     4201 acccaagtat cattttcatt taattctatg tttatattca tattgtattg agaaagagat
     4261 aatgcataag ttcctccaga accataaaaa gaatatgata atttctctct tatatattct
     4321 gtagttatca ctgggactat aaagcttcta gtattactat ctaaatttat tcttatatta
     4381 gtatcttcat atcttggttt taatgttgtt attaaagcat cagctataaa agcaaaatat
     4441 ctccaataaa actcaccttc atagttatct cttatacggt ctaacagttt tgtgccatca
     4501 ttttctaagc ttcttaaacc tggtgtccat cctgtttccc aagcaaatac tctatttgga
     4561 gcattaggta ataccattaa atcttttgac aaatcaagtt cttctttttg tacttccaat
     4621 acgtcatata tagataagtg tggctctcta tatgttattg atggtgctga aaagaagtga
     4681 tctatatcat cagttacagt atgacctgag ccaccttcca ttctccagat ttcacattta
     4741 cctaaagtta ttgaattgtt attaaagtct atctcagata ttactaaatc atcttgtggc
     4801 atcattattt tatcatctaa tgaagtaaat gctccttcag actcagctaa tgatatatga
     4861 ctaaaataat ctacaacttt tgttgcttta tctcttagga taagctcatt gtttactaaa
     4921 cttggtatac ctgctgaaat tcctgctaaa ggaactaaaa gtatactaaa tccactagct
     4981 attcctaaag atgaagtaat gattgcagtt gtagctgctg ttaaatttac tgccattata
     5041 cctatcttag cttctatttc ttgtcttaat aatgggtcac ttgtttcact tagctctttg
     5101 attgctgcac ctaaacttac accatctatg atagttgcaa ttacaggtaa tccttcagat
     5161 aatgtaggaa gtaaatctat agtttcatct aatgcagttg atactaattc aacaactttg
     5221 gctgcatctg taatagtatt taaaccagta ctaaataatt gagcataaac ttgaactttc
     5281 attgctacac ttaaattact aagagattct ttagaactat tatattctat taatgattgt
     5341 ataaaaaatg cagcatttaa agtattcact tcatgtgtag catctaaatt tacttttttt
     5401 actaacttac catttacagt atcaaatata gtacctttta tcttagaaat ctcttcagtt
     5461 atatgattag catattcaga gaatattgcc ttttcagttt ctacaaatat agattctcca
     5521 gtttctttat caataaatct tatactaaag ccttcatcag tctccaatat atcctcaaaa
     5581 gatataaaat gagactcttc taattcattc tgttgtttta aatcgtatag tgcatcagaa
     5641 atagattcta ttagtttaaa ttcattcttt atataattaa tagagtcaga agttaagctt
     5701 ttagcttctt caatccttcc ttctaccact tgtgtatcta tatttgaaat aacatttatc
     5761 tcacattctg ctaacattac tttttcttct agttcaatat cacttgaatt agaattgttt
     5821 ctaatttctt gtaataatgt agataattca ggtaaatttt tagattttac tataatttta
     5881 ttttctttag gattaaatga tatatattct tttgatgaaa tatcctttat aatactttct
     5941 tctttattta tccattcacc agaatgatcc aataattctc ttcttccttc actatttatt
     6001 ctaacttcat attgatttgc acttactata atagagtctt gacttataga tggcattaat
     6061 tctgatactt tatctttaac tctaagtaat aatttcccag gataagtctc ctctacattt
     6121 acagaatagc taaacatgtt acatcccagt aagtttattt ctattgactt aggagaaata
     6181 tcctctttag ctaaatctat tgctgtttct atttctgtgg ataatgaatc tacatcaaga
     6241 cctgcaaata tatcagtatt aaattcatct ttaccatgac caataaatgt taatttaatc
     6301 ttaggtctat cagaaattat acttggaatt ttatacttgt ctatttcttg tatttcacca
     6361 tctccaggat tataataata tgcaatttct gaatcttcta tatttttctg aaacagtaca
     6421 ctatcataag gagtctttgc aaataagtta catgctgctt cataactaat tttatctcct
     6481 tgtaactgaa caatatagtg tatatatcct ctctctgaac ttcttgctaa tgaagatatt
     6541 ttttctaaaa gatactcctt gtcaactact gtattttgag aaaagtcaag attgtcatct
     6601 tctccaagag aaccttcaaa gtaatttttt ttgtattctt caaattgagc tttagctctt
     6661 gcatcgtcaa atgaccataa gctagccatt tcttgttcag ttgattgaga aatattagtt
     6721 ttagagattt caaagtttct taagtcagct tctatcaaat ggatattcat actaccttct
     6781 ttaaacatta ataaatcttg ataagctgcc gcatatgctt cagggccact taagttaata
     6841 gtagttttaa catctgggaa gaaaccaact cttaaatact ttcctagttc catcataaat
     6901 ctaccattat ctgcattagc ttcagccatt atactatcaa taaaagcatt cgttgtagta
     6961 ttgaaatcgt tatcctcgct aatagctgga tttaaactat tattcaatat cttatatctg
     7021 ttctcgattt gttttactat taaattgcta caatatgagt ctttcacaga aattagtcct
     7081 tgatttataa tacctttact attaaatgca attttaactt ctagtggtga tgcctccata
     7141 tcgccaagtg atgagaatat ttctgactta tctgacttag aagctagaac agattcaaaa
     7201 ctactttgaa cttcttcatc caacatatca aaatgctctg aagtatatcc tggtatgtat
     7261 tctttgtatt tcattatagc ttctaatttt accatttccc aaaaatccac tgttactgaa
     7321 ctaggtttct ctatagactc aaataggtct ggttgtattc ctggtaacat atcaacatct
     7381 aaatatacac caccaacttc ttttaaggca gatattctta atatgtcaga agcagctgcc
     7441 aaattccatc tttctaccaa ctcttgttca tataatttga atgactctcc acctttaaat
     7501 tcttcaaagt ttctaacatc attcccacta ttttctgtaa ctttatttaa tgactcttca
     7561 atataggaat taagttcatc tatatccttt gaatactcgt ttgaaagata tatctttaca
     7621 atatcatcaa ttataaggtc aggattttct tctctttgag ttttatagta atttatgaaa
     7681 tttttctgct tatcatagat tatctccata cgttttctgt aaaatttatt atagtcaaat
     7741 ctagggtcat ttaagttttc tctaaatgat tcaagtgtat catttgttgc tgattctact
     7801 atagtttttt tcaatgtgtt tatcaaaaat gcattactat cataaaaaac attaacatta
     7861 taatcactat ttacatcttt ccattgattt atataattaa tagcagtgtc atttatttga
     7921 cctccaatcc aaacaaaatg taaatttttc tcaactggag ttaaattatt attctttagc
     7981 tctaatactt ctgtaactag gtattcctta aattttttta aggctttatt tctaccagac
     8041 tttttatatg tatctatata aatatctgtt aaactattta tatcttttaa ttttaaatat
     8101 ttttcgacta cagtattctc tgacatatta tgatattctt ctaaagcatc caatattgct
     8161 acatattcat cttcctgaac acgaaatctt acatttgcca ttttttctaa ctgttttcta
     8221 ttaactaaac tcataaaatt ttctccttta ctataatatt tttattgaat atttttacat
     8281 ctaaatgcta aaactctttt atatatcctc ctttctattt ataaaatata ctaatatcta
     8341 cttaggtttt catatacatt cacttcctaa cattttaaat tgaagacact cagttgatta
     8401 atttgctctt cgattgaata aagacgttgt ctgaaataaa tatgtaaact ttgttct
//
LOCUS       DACTGW010000165         8448 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.165, whole genome shotgun sequence.
ACCESSION   DACTGW010000165 DACTGW010000000
VERSION     DACTGW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.165"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            269..1429
                     /locus_tag="I9573_002786"
     CDS             269..1429
                     /locus_tag="I9573_002786"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAT4797395.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKRSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            1431..2561
                     /gene="glgD"
                     /locus_tag="I9573_002787"
     CDS             1431..2561
                     /gene="glgD"
                     /locus_tag="I9573_002787"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HAT4797396.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGS
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            2590..4032
                     /gene="glgA"
                     /locus_tag="I9573_002788"
     CDS             2590..4032
                     /gene="glgA"
                     /locus_tag="I9573_002788"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAT4797397.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNRDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            4046..6466
                     /locus_tag="I9573_002789"
     CDS             4046..6466
                     /locus_tag="I9573_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HAT4797398.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNIDNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYEQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            6471..8336
                     /locus_tag="I9573_002790"
     CDS             6471..8336
                     /locus_tag="I9573_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HAT4797399.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDIPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVDENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
BASE COUNT         3381 a          815 c         1571 g         2681 t
ORIGIN      
        1 tttttatatt tataaaataa aatttgtatt attttttaac cgataattga atattaataa
       61 atactatagt gcttatggaa aataatgtat acgtcggtaa gttcattatg gggtatacat
      121 tatatcaatt gtatagttga tattatttta tttttattaa ggtattattt aagcaacatt
      181 tatacacggc atttattgac tgataaagtt tataaaagta aaaatgcaat tataaatatt
      241 aattactaga tgaggggagt gtcattaaat gaaaaaagag atgttagcta tgattttggc
      301 aggagggcaa ggttcaagat taggtgtttt tacaaaaaga attgccaaac cagctgtatc
      361 atttggaggt aagtatagaa ttatagattt tgtgttaagt aattgttcaa actctggtat
      421 tgatactgta ggtgttttaa ctcaatatag accgttgata ttaaattcac atataggcat
      481 gggaagtcat tgggatttgg acagaataaa tggtggtgtc tatgttttac aaccatttat
      541 gaatgaaaaa gaaggaaatt ggtataatgg tacagcacat gcgatatatc aaaatatgga
      601 ctttgtagat acctataatc cagaatatgt attgatatta tctggtgacc atatatacaa
      661 gatggattat agcaaaatgt taaaatttca taaggaaaaa cgctctaaag ctacaatagc
      721 agtgattgaa gttccttggg atgaagcttc aagatttggg ataatgaata caaatgaaga
      781 ttcaagtatc tatgaatttg aagaaaaacc aagtgaacct aagagcaatt tggcatctat
      841 gggagtatac atatttgatt ggaaaatgtt gagaaattat tttaaggaag cagaaaaaaa
      901 tccagaaata aattatgatg attttggaaa aaatttaata cctaaaatgt tagaagataa
      961 tgttggaatg tatgcttatc catttaaagg atactggaga gatgtaggaa ctatacaaag
     1021 tctatgggat gctaatatgg atataataaa atctccagaa acacttgatt tagcagaccc
     1081 taaatggaag atatatacaa atactatggc aatgcctcca caatatatag gtaaaaatgc
     1141 aaatgttcat agatcaatga tagctgatgg ttgtagaatt ttaggtgaag taggcaactc
     1201 tgtattatca catggagttg ttgttggtaa aggaagtaaa gtaattgatt cagtaataat
     1261 gccaaatgtt gtaataggtg aaaatgtaac tatagaaaaa gctatgatag gtgagtgtgc
     1321 aactataaat gataatgttc agataaagaa tgtaaataat gaaataaatg tagtttctga
     1381 gtatgaaaat atcgaaccta gatgcgtatt aatagaagga ggtctttaag atgagaaatg
     1441 aatgtttagg gataataaat ttaaacaaaa aaggagaccc agcaataaac aaacttaact
     1501 atggaagacc aatagcatct acaccaatag ctggaagata tagaataata gactttgctt
     1561 tatctaatat gataaattca ggaatcacta aagttggaat attcgcaaaa gaaaaataca
     1621 gatcattgac agaccatata ggtagtggta aagattggga tttaagtaga aaaaaaggtg
     1681 gcctatcaat ttttagtcca gaaaacacta agtatagaaa tacttactca catagagaag
     1741 gtgatatata tactatctta gctaatctag attatataga aaagagtgaa gaagaatata
     1801 ttttaatagc tccaagctat atgctatgca atttaaatta ttctcaagca ttggaatacc
     1861 ataaaaaatc tcataatgat ataactataa tatataaaaa tgtaaataat gcaaataaag
     1921 attttgctgg taacttaaca ctaaatttag atagtaacaa tagggtaata aatgtaggaa
     1981 ataatttagg caaattccct agagcaaaca tatgtatgga gacatatatt atgaagcgtg
     2041 aagattttgt tgaatgtata tataacattg taaataaagg cagttattgt tatttagaag
     2101 aatttattat tgaagaagca gaaaatatga aaataggagc ttatgaatat acaggatatc
     2161 taaaatgtgt aaattctgtt gaatcatatt ttgaaatgag caaagattta ttaaatttag
     2221 aagttgcaga tgaattatta tactctgaaa gaaagatatt tacaaaagaa aagaatgaat
     2281 cacctacaat ttatacagat agtgctaaag tagaaaatag ctttatagca tctggttgtt
     2341 taatagaagg tactgtaaaa gatagtatca tatttagaaa ggtaaatgtt gaaaaaggaa
     2401 cagttataga gaactcaata gtaatgcaaa attgtgtaat aaagtcaaat gcaaaactat
     2461 ataatgcaat attagataaa aatacgagtg tatcaaatgg gaaagaatta agaggagatg
     2521 aaaaatatcc tcttgtagtg gaaaagaata caaatatata aataaatttt ttagaagggg
     2581 gaatgactaa tgaaggtttt ttatgtaaca gcagagtgtt ggccctttgc aaaaactgga
     2641 ggattaggag atgtttctta tgcattgcct aaagaattaa aaaaagaagg tgtagatgta
     2701 agggtaataa tgcccaaata ttcaactata ccaagttatt taaaagatca actaaaagaa
     2761 atagctgtgt ttagtgtacg tgttggatgg agaaatcaat actgtggtct tttagaaatg
     2821 gaattggatg gtgtaaaatt ttactttata gataatgaat tttattttag aagagaagat
     2881 gaaagaaaga gtatctatgg atatggagat gatgcagaaa gatatacttt ctttacagat
     2941 gcagttttag aagctataag tagaattgat ttttatccag atgtaataca tataaatgat
     3001 tggcatacag ggatgttacc attaattcta aaagaaagat atgcaaccct tgaggggtat
     3061 aaaaatataa aaactatgta tactatacat aatctccaat atcaaggtgt atttgataag
     3121 catgttttat atgatatatt ggacttacca caaaaatact ttgataatag agatatagag
     3181 tattatggct caataaattt tatgaaagct ggtataaatt ttgcagataa aattataact
     3241 gttagtccta cttatgcaaa tgaaatacaa acatcttttt atggtgaaca attagatgga
     3301 ttacttagaa aagagtcagg aaaattaaaa ggtatattaa atggtataga ttatgattta
     3361 aacgaccctg caaaagataa agatatattt gttcattatg atgtagactc aattgacaaa
     3421 aaagtagaaa ataaattgag attacaagat atattgggac ttaaaaaaga ttcatctatt
     3481 ccgttgatag gtatagtttc gagattagtt tctcaaaaag gatttgattt aatagcatac
     3541 atgatgccag aacttatgag agaagattta cagattgtcg ttttaggaac tggagaacat
     3601 caatatcaat caatgttcaa ctattacgat tctaattttt cagataaagt atcagctaga
     3661 attactttta atgcatcatt ggctcaacag atatatgctg caagtgatat gttcttaatg
     3721 ccatcattat ttgagccttg tggtataggt caaatgttag ctatgagata tggttcttta
     3781 ccaatagtta gagagactgg agggcttaga gatacagtaa caccttataa taaatttact
     3841 ggagaaggca atggatttag ttttaaaaat tacaatgctc atgagatgtt tttctgtttg
     3901 aaaaatgcaa tcaaagtatt taaggacaaa gaaaaatgga ttaaattagt tgaaaatgca
     3961 atgaaaacag ataatagttg gaaaaaatca gctaaggagt atatagaaac atatagagac
     4021 atatgtgatt aaaggagtgg tttttttggt aacaataagc aaaaagaaat tcaagcaaaa
     4081 atttgaagta aaaatgtata gcttatatgc acaatctatc aaggaagcta ctgatgaaca
     4141 attgttaaat gtattatgta gtctattgaa agatgagata gcaaaaaaat gggttgctac
     4201 aaagttagat aagaaaaaag aagtttatta ttttagttta gaatttttaa tagggagaca
     4261 gttaaaatct aatttattaa atttaaatat tgaagaagaa gttagagaag gtttatctga
     4321 attaggaatc aacttggatg atttaattga agctgaagta gacccagcct taggaaatgg
     4381 tggacttggt agattagcag cttgcttttt agattctatg gcatctttga acataagtgg
     4441 tcaaggatat ggaataaggt ataaatatgg tctgtttgaa caaaaatttg taaatggtta
     4501 tcaggtagaa gtgcctgata actggcttac agaaggacga tatgcatggg aaactgtaag
     4561 accaaatgaa gcaacaatgg tgaagtttgg tggtgaggta gaattaatca aggaaggtag
     4621 tcatttaaaa gtaattcata aaaactacct tcctgtaatg gcaatgcctt atgatatacc
     4681 aataatcgga taccaaaacc aatgtataaa tactttaaga ttgtttaaga gtgaaatacc
     4741 aaagagagat tttggtgaac ttacctcaaa tgctctaaat tattctggta gctatgaaga
     4801 ggctttaaaa cataagtatt atactgagga aatatcacag gtattatatc ctgatgattc
     4861 aaactatgca ggtaagttgt tgaggttgaa acaagaatac ttttttgtaa gtgcagggat
     4921 acaagatata ataagaaaat ataaaaagaa caaattaaat attgataatt tattcgataa
     4981 ggtagcaatt catataaatg atacacatcc taccttatgc ataccagaac ttatgagaat
     5041 tttgcttgat gaagaaaatt tatcttggga tgaagcatgg gaaataacta aaaaaacagt
     5101 gtcctatact aatcatacta taatgtcaga agcaatggaa aaatggcctg taagtatgat
     5161 gaaagaacta ttaccaagaa tttatatgat aatagaagaa ataaatagaa gatatgtaga
     5221 ggaattaaat aataaaggat atgagcagga tagaataaaa agaatgagta taatagactg
     5281 tgacaatatc aatatggcaa acttatgtat tgtcacaagt catagtgtaa atggagttgc
     5341 taagcttcat actcatattc ttgaaacaga ggtattaaaa gatttctatc aagatgaacc
     5401 aaataaattt aataataaga ctaatggaat tgcacatagg cgatggctta taagttcaaa
     5461 tcctcaactt agcaatttaa ttacagattt aattggtgat tcatggaaga aggatacctt
     5521 acaattaaaa aatattgaaa aatttaaaaa tgattcctct gttttacaaa gacttgatga
     5581 tataaaatac aataataaag caaatctagc aaagtttata aaagataaat acgatttaaa
     5641 tgtagaccca agttcgatat ttgatgttca agttaagaga ttgcatgcgt ataaaagaca
     5701 gttactgaat atatttaatg tacttcatat gtatcatgaa ttacttgata atcctaattt
     5761 gaatcttgac ccaagaactt ttatatttgg tgcaaaagca gcaccaggat attatttggc
     5821 taaatgtata attaaattta taaattctgt tgcaagtact ataaataatg atgttagagt
     5881 aaaggacaag ttaaaagtag tatttttaga gaattatgga gtatcattag cagaaataat
     5941 aataccagca gcaaatgtta gtgagcaaat ttcaactaca actaaggaag catctggaac
     6001 aagtaatatg aagtttatga tgaatggtgc tataactttg gctacacttg acggcgcaaa
     6061 tgtagagata tgtgaacagg ttggtaaaga aaatatgttt ttatttggac ttagtgcaga
     6121 acaagtacta aattataata aatatggtgg atattcttca cttgacttat atcattctaa
     6181 tatggatata aaaagggtgg tagatgattt aataaatgga tttattccta atcttggcga
     6241 agaaggaaga agtatttata attcactcac tacttacaat gatgagtatt ttgtattgag
     6301 agattttgaa aattatggtc aagctcaagc agatataaac aggttatata gagataaaga
     6361 aaaatggaat aaaatgtctt tagttaatat agctaactct ggattttttt cttctgatag
     6421 aacaatttct gagtatgcaa aagacatttg gtttaaaagg gtgtgattaa atgcaaagtg
     6481 taatagaata taactcttgg gataaaaatt ttaaggctcc gtttggagct ttaaaatttg
     6541 atgaggaatt gaccatttgt gttaaagtaa atgagggata caatatcaag tctatttccc
     6601 tagaaataaa tagagaagaa gaaatgagaa caataacttt aaatgaagaa ttagataatg
     6661 ataaacttgg aaagtgcttt tgtggtaaga ttgaaaagtt tgatggtaca ggtgtttatt
     6721 tttattactt taaagttgat gttgaaatgg atggtcagat aaagacttta ttttatggaa
     6781 aaaacagaga taatggatat tcttgtgaat ataattatag tgatattaat aaatatcaaa
     6841 tcactgttta caaagatttt aaagtaccaa catggtacaa agaaggaata ctttatcata
     6901 tatttgtaga tagattcaat aatggaaatc gtagtggtaa ggttgataat cctaaaaaaa
     6961 atagtttcat atatggaaat tgggaagata tacctatgta cataaaggat agtcaagggg
     7021 atgttataag atgggatttc catggtggaa atttaagagg gataatcaat aaacttggat
     7081 atttaaaaaa attaggtgta agtatactat atctaagccc tatatttgaa gcttctagta
     7141 atcataaata tgacacaggt gactataaaa aaattgaccc aatgtttggg gatgaagata
     7201 catttaaaga acttattgat aaagcaaaag aaaaaggtat atcaatagta ttagatggag
     7261 tttttagtca tactggagca gatagtaaat attttaatat gtatggaaac tataatagtt
     7321 taggggcata tcaatctaaa gaatctccat attattcatg gtatatgttt gaagaatttc
     7381 ctcaaaagta taaaagttgg tgggatgtta agacattgcc aaatataaat gaattagaac
     7441 atagctatat ggattatata atatatgaca atgatagcgt tataaataaa tgggtaaata
     7501 tgggtattaa gggctggaga ctagatgttg cagacgaatt accaactaag tttataagag
     7561 agttaaaaaa ggaattaaaa aaggcagatg atgactcaat tcttataggt gaagtatggg
     7621 aagatgcatc aaataagatt agctatggtc agagaagaag ttatttatta ggtgaagaat
     7681 tagattctgt tatgggttat ccttttagaa ataacatgtt ttctttctta aaaggagaaa
     7741 ttaattcata tgaactttgt aatagatata tgcagattaa agaaaattat ccaaaagaat
     7801 catttaggtc taatttaaat ttaattggaa cacatgatgt tacaagagct aaaacggaat
     7861 taaatgatga tgtagattta gtgaagcttg ctgtagctat acagatgaca tttgaaggag
     7921 taccatatat atattatgga gatgaagcag gtctttgtgg aggtgttgac ccagataata
     7981 gaagaactta tccatggaaa aatgaagacg aagatatgct taatttttat agagatgtta
     8041 taaaaattag aaataagaat aaacttttaa gttctggaaa tacagaattt atatatacta
     8101 ataatgattc tgtattttct tttattagag ttgatgaaaa taatgataga atgttaattt
     8161 tagtaaatag aagtgaaaat gtggaaagta tatcattatg tatagagagt agttttattg
     8221 aggaaatacc tataaagtat agtcttaaaa atgcaaatag tactatacaa attgaaaata
     8281 atgagttgaa agtagattta gactctaaat catttagaat ttttagagta aattaaatat
     8341 ctttttttag aaaaataaat agaaaataag gtgaatgtag aaagagtttt gttttatcaa
     8401 aactttttct actttttttg tataaattat cataagaatt ttagtgtt
//
LOCUS       DACTGW010000166         8369 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.166, whole genome shotgun sequence.
ACCESSION   DACTGW010000166 DACTGW010000000
VERSION     DACTGW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8369
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.166"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(242..1669)
                     /locus_tag="I9573_002791"
     CDS             complement(242..1669)
                     /locus_tag="I9573_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HAT4797400.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSINKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKLVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTIAKNNAIVLVGNDSNKSV
                     LKSTIKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKIYN"
     gene            1860..2681
                     /locus_tag="I9573_002792"
     CDS             1860..2681
                     /locus_tag="I9573_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAT4797401.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETK
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKSLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            2806..4323
                     /locus_tag="I9573_002793"
     CDS             2806..4323
                     /locus_tag="I9573_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAT4797402.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKACDLDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAIYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            4346..4630
                     /locus_tag="I9573_002794"
     CDS             4346..4630
                     /locus_tag="I9573_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAT4797403.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(4727..6391)
                     /locus_tag="I9573_002795"
     CDS             complement(4727..6391)
                     /locus_tag="I9573_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAT4797404.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(6463..7710)
                     /locus_tag="I9573_002796"
     CDS             complement(6463..7710)
                     /locus_tag="I9573_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAT4797405.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRAPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            complement(7697..8362)
                     /locus_tag="I9573_002797"
     CDS             complement(7697..8362)
                     /locus_tag="I9573_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAT4797406.1"
                     /translation="MVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVSDLL
                     KVLLKVQKPKRILEVGCAIGYSSIFFASIIGKDADIITVERNEKMIEKAKENIKLAGF
                     DNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDNILY
                     KGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRGDVD
                     GIK"
BASE COUNT         2892 a         1353 c         1033 g         3091 t
ORIGIN      
        1 gcgagagtgg ctttttgctt ttttgaatag tttactgatt aagtaaacta ccacactagc
       61 tgttaaacta gctaaaacat taaacaaaaa gttatccatg aactcacctc ccttcgaatc
      121 gttgggagga taatcttttg tacatgaact ccactctata gattgtaagt taaatcttct
      181 tgctaaaaat attataacat attatggtaa attatataat tattgtttaa aagttctaat
      241 tttaattata aattttccca gtttctatat ctataaaaaa ccaaccaaaa gtaccattac
      301 tattttttga attgatttta acagcataga cattaccact atatatatca tctctaaaat
      361 ctatcatatc aactactaag ttattgatac tgactttatg tctcttagca cattctttta
      421 atgctatctg ttttgcttgt tcctctgtta tacttccttt atttttataa tcatctggat
      481 tacatacttt catattacca ttaacatcat atgtataaac tttgctcata tcatttttag
      541 ctacacagta tctaacatca ccctctgcac cctgtttaat gtaagcaaat acatagtact
      601 cttttcctga aaaatcaaaa tttttatcta gtagaaatat taaatcttca tcttctgaat
      661 aattaggttc gtaataaaat ttcttcacac ctaatagttt tttcactgca ttagcagcat
      721 cttctactgt atattctctt ccagtattag aatcgcctat attttttgtt acatttaaac
      781 actgctgtaa tatactatca ctcaaatttc ctatagcagt taatttaatt gtacttttca
      841 atacactttt attactatca ttaccaacta acacaatagc attattttta gcaatggtag
      901 aaccaacaag agcatacact aaatcagtac cacttgctat ataaaattct tttactccac
      961 tataaaattt atttattatc tttttgttag tatcatatct atctgtccca cctaatctag
     1021 ttgaattagt atccttaact agtttgtcat tcattgatga agtaccacct attacataac
     1081 tttctacacc agttgtatta aatggaacac tttttccatc agttaatact atagctgctt
     1141 tatctctaac tgctacaggt gcagcactca ttgcatcagg ctctccttta aaagcattag
     1201 tcaatatgac tttatttact ttgttaattg aatttatttc ttttgcaaca ttatagcttg
     1261 tcttaattct atcactacct tgaagtcttt taatctctat tcctttgcct tttagaacag
     1321 tttcagtata tttattaata gagttttctc ctccaattat atatactttt tttgcctttt
     1381 ctaatctttt aagtgtagca tttggtatgt tatttttctt agtcaaaaga attggagcgt
     1441 ttatagctcc tgcaagtcca cttgcactta atccatcagc cattgtactg tctgcattaa
     1501 ttaatatagc tgtagtatag ttttgtttgt ctgcaattat accagcagtt tcgtatttat
     1561 ctgcaccttt aattgattca attttatcaa gagcatttgc agatataggg caactagcaa
     1621 taaatataga taaagataac cctattgtta ataatttttt aattttcata atatctcctt
     1681 ctcttaaata ttttcaccta aattatataa tacttttcag gaaaatgtta ccataaattt
     1741 taaaaattat tattacttaa tataatatta atgaaaaata tcattcttaa attaatgact
     1801 ttggtagaat atggtgataa aataaaatta gtgagagaca atataagggg gattatatta
     1861 tgataatcag taaaaaaata acagcaatat tagtatcatt tttaattatg atatcaatgg
     1921 ttggatgtgc tccaaaagaa agtgctacag aagtagctaa aaagtacctt gatagtatag
     1981 aaatcaaaga ctacgaagta gcatataatt tattaagtaa agacagtaag gaaagtgtca
     2041 gtctagaaaa atttgaagaa tatcaagata ctttatttaa acttcaaacc ctaaaagatt
     2101 ataaacttgg tgaagaaaat aaagtagaga aatacaaata ttctaataaa gagtacacaa
     2161 atcttgttaa aataaaagaa actgatacta taaagtatat cactgaaaat aaagaagata
     2221 ctgcaaattt tgatagatat tttataatcg aagataatga atataaatta ttatggcata
     2281 aagactttaa aagagctctt agctctaatt atgtcaaaat cgctaggtca gaaacaaaaa
     2341 aagatgaaga taaggattat aataaagctg ctattgatat caaaaaagct atagaaattg
     2401 atgataaaaa tccacttgca tattatacac aatcatatat ttatatagag ttacaacgat
     2461 atgatgaggc tttaaagtca attgataaat ccttagaata tctagataaa aataatgaag
     2521 cttataaaat agacgctagt gatgcttaca atttaagagg tgttattttt atgagtaaat
     2581 cagaatttaa taaagctaga gaatcttaca ataaggcgct tgaacttaac ccggataatg
     2641 aatatgcaaa aactaatcta aatgatatta aaggattata aggcattaat attatagaaa
     2701 ataagtatgt tgaaagttaa attaactttt gacatgctta ttttctatat tttaaactct
     2761 attgatttac tatataatag tgcataagga gtgtgattac ttattatgag tgttgccata
     2821 tacttaagaa aatcccgtgc tgatgaagag gcagaaaaac aaggcgaatt tgaaacttta
     2881 agtagacata aatcaaccct actaaaacta gcaaaagaac aaaacttaga tgtaattgag
     2941 ataaaagaag aattagtttc tggtgaaagt ataatacata gacccaaaat gcttgaactt
     3001 ctaaaagaag ttgaagaaaa taaatatgat gcagtcttag taatggactt agacagactt
     3061 ggtcgtggag acatgaaaga ccaaggtatc atattagaaa cattcaaaga aagtaaaacc
     3121 aaaatcatca cacctagaaa aacatacgac ctaacagatg aatttgatga ggagtattca
     3181 gaatttgaag catttatggc aagaaaagaa ttaaaactta tatcaagaag aatgcaaaga
     3241 ggtagaataa aatcagttga agaagggaat ttcataggaa cttctgctcc atttggatat
     3301 gatgctgtaa caacaggtag aaaagaaaga atacttgtac caaataagga tgctgatgta
     3361 gttaggacta tctttgactt atatattaat gaagatatgg gttgtagtaa aatatcaaaa
     3421 tacctaaata acttaggcat taaaactgct acaggtgcta attggtacaa ttctgcaatt
     3481 acaaatataa taaaaaataa ggtatattgt ggctacattc agtggcaaaa aaaggactat
     3541 aaaaaatcta aaaatccaaa taaaataaaa actgttaagc taagacctaa agatgaatgg
     3601 attgaagcaa aaggaaagca tgaaccttta ataagtgaaa taacatggaa aaaagctcaa
     3661 aatatactta aaaaaaacgg tcatgtcagc tatggtaatc aaataaaaaa tccacttgca
     3721 ggaatagtga tttgcaaaaa ttgtggtaga ccattagtat atagaccata tgcagaccac
     3781 gactatataa tttgttacca tcctggttgc aataaaagtt ctcgatttga atttattgaa
     3841 gctgccattt taaaatcttt ggaagacacg gtaaaaaaat atcaattaaa agcatgtgat
     3901 ttagacttag ataaaaataa taaagatagt aatatagaat ttcaaaaacg agtcttaaag
     3961 ggcttagaaa ctgaattaaa agaattaggt aaacaaaaaa ataagttata tgatttatta
     4021 gaacgtggaa tatatgatga agatacattt attgaaaggt ccaacaatat aagttcaaga
     4081 actgaagaaa ttaaagattc aattaagaca gtaaaaaata gattgagtac tgttaaaaaa
     4141 gacaattcta aaataataga agatattaaa actgtgttat cactttatca tgattcagat
     4201 tcccttggaa aaaataaact cctaaaatct gtaattgata aagcaattta ttataagtct
     4261 aaagaacaaa aactagattc ttttgaatta atggttcatt tgaaattaca tgaagaccag
     4321 taataccaat aacatataaa gagtagtgtg taatgaaata agtcttttag atatacttgg
     4381 cacagaggct aactatgttc ttgatgaagt tgaactaaaa gttcaagttg gaaaacttta
     4441 cgaacaacta aacaaaattc ttactcctag agaaagagaa attgtccagt taagatatgg
     4501 gttaactcct tatggctata agacacaaag agaaattgca caaaaactcg atatatcaag
     4561 gtcatatgta tctagaatcg aaaaaaaagc tcttaaaaaa ctagaaaaag agcttgttca
     4621 agaaagttaa tactatattt taaaaggata tatcgtacat cattagacaa agttttctat
     4681 ctaaaatcaa agttacgata tatccttaaa ttttatagta cataatttat tttatacttt
     4741 cacatatttc cttaaatata ggtgttgcag atttatttcc tttttgagtt ccttctacta
     4801 acacagttat tacatactta ggtctttctt caggataaaa tcctgttatc catccgtggt
     4861 ctatagcttt tttatttaaa ctactttgag ctgtaccagt tttaactcca caaccacctt
     4921 caatatcttt taggtcttta gctgtaccaa tcctagatac agacttcata tattctttaa
     4981 cttgtgtaca cacataagga gaaataagtt cttctttttt tgaagactta taagttttta
     5041 ttgtattcat attattgtcc acaagacttt tatataagta tagtggtttg aatgttccat
     5101 tattagctat tatttgagtc atttgattta tttgaagtgg tgtaaattct atattttctt
     5161 gacctatagc taaatttctt attgatatgt tttttggagc ttctctaatt ttttcttcat
     5221 caagaccaat atcaaccttt tcaaataaat gtaatttttc agcagatttg agtatctttt
     5281 cttttccaag tttcatagct atatctaaaa atgcaggatt gcatgaatta gaaaaagctt
     5341 gtcttaagtt ttggaaacca tgaccatctc ttttattaca tcgcagtatt tcattagtat
     5401 tcccaacttt agtcttacct gtacagttgt aagtatagtt ctcatctata acaccatttt
     5461 caagtgcaga aaataaaact accatcttaa atacagaccc tggtggatat gtcgctttta
     5521 taactctatt ttcaaattcc ccattttttc cttttaaact ttttgatatg tcattttggt
     5581 caaaattagg tctagagcac attgcaagaa tttcacctgt agatgcttcc gagattattg
     5641 ctgctgtagg attttcttct ttatttagta tttgttctaa ttttttttga atgttataat
     5701 caatagtagt ttttagatgt ctatctttat ccttattatc aacagttttt acacttccct
     5761 ttagtatgtt tagagatttt tcatttcctg catctccagc tttaaaagca cttatatact
     5821 tttcatttga atttctaagt acactatcca tactcttttc aattcctgat tgacctgatt
     5881 tatcatcttc ttttatatat cctattgtat gtgataataa accatctttt gcgtacctca
     5941 tagttttttc ttctaccatt attccatttt tctcaagttc ttttttcata ctcttatcga
     6001 tatttttagt ctgaatctgt attatagggc gtgtcaattg ctcttgtaca gctttatata
     6061 tatctaaatc attcatctta gtagctttct taataagttc caaaatttta tagttattat
     6121 ttaatttttc tttttcaaca attaatacct gagatttact tgtatctgta agcttcttgt
     6181 tgtttctatc atagattatt cctcttccac tatttaactc gactttctca acgctttgtg
     6241 attcaacact ttgtttgtat ttatcacctt ttaatacttg tatatcaact agcctataaa
     6301 ttaaaacact ataaattatt aaaattatag taaatataca ccagcttctc tttcccactt
     6361 ttttcaaaaa tggagtcttc ttttttgaca tgaaatcacc ccttagataa tttcattatt
     6421 gacaatttct aaggggctaa tactattttt atcaattttt tattattctt caaccttttc
     6481 ttctataact tttctaagca tataatcttt accaactttt actgatagct ttaacttcac
     6541 tatttgtcta ggatgaggag ctgattctat agcttctcct tcttcattgt acatttcttc
     6601 aattttagta aacatagttt ctttatatgg tcccataact tctatttcat caccaacaca
     6661 cattttattt ctttgctcaa caacaataag accatcttca tcttctgttt ctctaactat
     6721 acctataaaa tcatagtttc taacatatga tgctgattga taattttggt cttctgttgt
     6781 tggtttattc aagtaaaaac ctgatgtaaa atgtctatga ctaccttttt ttagttcttc
     6841 catccacatt ggattgaact tccagttttc tggggctctg taaaattcat ctattgccat
     6901 tctgtatgct cttacaacag tagctacata gtatgcagtc ttcattctac cttctatttt
     6961 taaacttgtt ataccagatt tgattaactc tggtatatac tctatcatac ataaatcttt
     7021 tgagttaaag aagaatgtac ccctttcatc ttcgtatatt ggaaaatact caccaggtct
     7081 tttttcttcc accaaatgat attgccatct acaagattga gcacaagaac ctttgtttgc
     7141 atctcttcca gtcatatagt tgcttattaa acatctacca gaataagaaa tacacatagc
     7201 accatgcata aacgcttcta tatccatatc taaaggtgtt tttgctctta tctcagatat
     7261 ttcatcaaaa gataattctc ttgccactac aactcttttt attccatgct cgtaccagaa
     7321 gtttgcactt agataattgg ttgtatttgc ttgtgtactt aaatgtattt ccatattcgg
     7381 aattactttt ttaatgacac taagcactcc tgggtcagct attataactg catctattcc
     7441 tatctcttct agttccaaca aatagtcttc taattgtaga aaatcttcat tatgaggtat
     7501 tatatttact gttacgaata ctttcttgcc tctttcatgt gcaaaagcta ctccctctgc
     7561 catatcttcc ttactaaaat tcttcgctgc agacctcatt ccaaaaattt ctccacctat
     7621 ataaacggca tctgctccat atgttatagc tattttcaat ctctccaaat cacctgctgg
     7681 agctaaaagt tctactttat ttaattccat ctacatcacc tcttttactt tctttataac
     7741 ttagtgctac accatcacct attggtacca atgatgtgct taaatagtca caattacata
     7801 tataatctaa ataatttctc attcttttaa ctatagtctt ttttcttctt acaacaaaat
     7861 catcatgagc aaccatacct ttatacaaaa tattatcaga tactaaaagt cccccatctt
     7921 ttaatttatc tataactaaa tcaaaaaata gcttatattg acctttggct gcatctataa
     7981 atataaggtc aaattcacct tggacttgac taagtttttc ttctgcatct ccttctagta
     8041 tagttatgtt attgtcaaat ccagctaatt ttatgttttc ttttgctttt tctatcattt
     8101 tttcatttct ctcaacagtt attatatctg catcttttcc tataatactt gcaaaaaaaa
     8161 tagaagaata accaattgca catcctactt ctaaaattct ctttggtttt tgaaccttta
     8221 acaagacttt taataaatct gaaacttctt tatgtattat aggaacacta ttttcctctg
     8281 cataaatttc taaatctttt aaaaatcctt ctttttcttt taatgtcact cttatataat
     8341 cttcaaccaa atcattaact atattactc
//
LOCUS       DACTGW010000167         8346 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.167, whole genome shotgun sequence.
ACCESSION   DACTGW010000167 DACTGW010000000
VERSION     DACTGW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.167"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(17..778)
                     /locus_tag="I9573_002798"
     CDS             complement(17..778)
                     /locus_tag="I9573_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT4797407.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            997..1473
                     /locus_tag="I9573_002799"
     CDS             997..1473
                     /locus_tag="I9573_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAT4797408.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1571..1723
                     /locus_tag="I9573_002800"
     CDS             1571..1723
                     /locus_tag="I9573_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HAT4797409.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(1883..2071)
                     /gene="rpmB"
                     /locus_tag="I9573_002801"
     CDS             complement(1883..2071)
                     /gene="rpmB"
                     /locus_tag="I9573_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAT4797410.1"
                     /translation="MAKVCSVCDKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            2432..2782
                     /locus_tag="I9573_002802"
     CDS             2432..2782
                     /locus_tag="I9573_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAT4797411.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            2801..4444
                     /locus_tag="I9573_002803"
     CDS             2801..4444
                     /locus_tag="I9573_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAT4797412.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            4525..6582
                     /gene="recG"
                     /locus_tag="I9573_002804"
     CDS             4525..6582
                     /gene="recG"
                     /locus_tag="I9573_002804"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAT4797413.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIRNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMERFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            6641..7195
                     /gene="rsmD"
                     /locus_tag="I9573_002805"
     CDS             6641..7195
                     /gene="rsmD"
                     /locus_tag="I9573_002805"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAT4797414.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNVKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            7197..7694
                     /gene="coaD"
                     /locus_tag="I9573_002806"
     CDS             7197..7694
                     /gene="coaD"
                     /locus_tag="I9573_002806"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAT4797415.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            7698..8222
                     /locus_tag="I9573_002807"
     CDS             7698..8222
                     /locus_tag="I9573_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797416.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
BASE COUNT         3304 a          924 c         1465 g         2653 t
ORIGIN      
        1 cctttattat tttatattat ttatctcttg tatatattac tatagcatcc cttaaaaact
       61 tagctaatcc aggttgttct ctatcataat atgatgtaaa acgttcatca tatacataca
      121 tctgtgctag agaggaatga gcttctttag agtatgtgtt ccaagtgtaa ctcaaccatt
      181 tatgatgtaa ttttgcaact tcctgagcca gttcactaga ggcatcacca gttttaaaag
      241 cttttttaag tttactaatt atttcgatat ttaggttttg ccaattttca taatcttgct
      301 tagacatatt tttaagcttt ttatttgatg tatctataat atctttacca tatttctctc
      361 taatttcagt tccataattt ttttcatttt catcaatcat cttttcttta aaaccttcaa
      421 atttttctat atctgtcata gtgtattttc cctccttcag agctatagtt ttagttacat
      481 tttctattag taaatcaatt tgctttcttt tggctagtaa tttattatgg tgttctttta
      541 acgcacttaa actatcgaag tttggtgaat ttataatgtt ttttatattt tctaagttaa
      601 ttcctaattc tctataaaaa agtatttgtt gaagtttatt aacttcattt tgaccatata
      661 tacgataacc tgaagaattt attttaagtg gcttaagaag acctatttca tcataatatc
      721 taagtgttct agtacttata ccagctagtt tacttaattt ttgaactgta tattccataa
      781 gtaatacctc ctataaatta aatatacact tttacgcaac gtaaatgtca atatattaaa
      841 atataaaaat ttatatcttt taataaaaat ataaaaaaat atctttactg gatattttac
      901 acacaactgt caatactcac taatgaaaat taaaaaataa aatttataga ctatctttac
      961 taaaatatag aaggtctata gcctaggagg aactgtatga atttaaaaag aagcaatgaa
     1021 aaacgagaag agtacagaag aatgtattca gataaagaaa gttttttaag tttgattcaa
     1081 aattttacaa gtgtctcaat agcaaaagaa ttaactctta aaaatataga gttagaaaca
     1141 tcttttatat gtgaatataa aggaaaagaa gttgatataa tttataaggt attttctaaa
     1201 agtggcaaag tatcacatta tatagtgtta gaatttcaaa cagaaatgga tactgaaatt
     1261 gtcccaagat tgaaatcgta tagagaacaa atttggaaaa gttttattat gaaaaaaagc
     1321 cttgaagaaa tagaagataa aaattttaaa cttcctaaag ttataccagt ggttttatat
     1381 agtggacctg aaaggttaag tagaaaaaaa ggaattttgg atattgtaga atctatatcg
     1441 gagttcagta ataattctag tatgaaaaat taaaattaca taatgtgttt attggtctaa
     1501 aatggaaaac tgtcgcataa acttgaatat gaaaatattt atagatacag gttctatgtg
     1561 gaggtgttcc atgagaaata acaagtctaa ggtattgcta ggaatacttt gtctagcaac
     1621 aggaatggct gtaattctgt caatgttgct tcctggctgg atttggtcag cacttaccgc
     1681 attaatactt attggatgtg gagcattatt atttttttgt taaaatagtt ttaactataa
     1741 agtgtggggt gagtattgat gaaagtagta acaataaaat taccaaaatg gatttcaagt
     1801 ataatcctaa aatttatgag aaagagtaaa aatgattaaa ttaaaaagcc tacttacgga
     1861 agtaggcttt taaacgtggt tactaagctc tttcaatttt accagaacgt aaacatctag
     1921 tacaaacttt tactctctta ggagctccat ctataatcgc tcttacgctt cttaagttag
     1981 ctgaccatgt tcttttatta tgtttatttg agtgtgatac ttggtttcca gaaaccttac
     2041 ctttatcaca tacgctacat acttttgcca tctacgcaca cctccttaaa atgataacta
     2101 tttaaagact aaaaacatat ttatattatc ataaataaca aaaatttgca atagaaatat
     2161 atatttgcct tacacatatt gaagaatttc ttattatcat ataaaattat atatataatt
     2221 ttatataaag taaatatatt tggaaaataa ttattatttt tctttgaata tatatactat
     2281 agaagaaatt ttatccaaaa acttttctag gtttaaatta taccatattt atagtatgat
     2341 atgattataa ttttatataa taaataagta aaaatagtaa aaaataaatt aagaaatgtt
     2401 tttaataaaa ataagcttaa gggggtcaac catgagtgca aaggtaacga atcaatatgg
     2461 aaatatagaa atagataatc aagtaatagc tcaagtcgta tatagagctg caatggaaag
     2521 ttatggctta gtaggtttag catataagtc taaaggaata gtagagctat taaaaggcga
     2581 gaacgcaact aaaggtgtaa gagttgagga agtagaagaa gatgccatag caatcgaact
     2641 ttatgttata atacaatatg gtacaaatat atctactgtt gccaataata taatagatag
     2701 agtaaagtat gtagttgaaa aaatgactgg cgtaagagtt actagaattg atattaatgt
     2761 acaaggaata agagttaagt aatttcatag gaggaaacaa atgattcaat atatagatgg
     2821 taaaaggtta agggagatgt ttatttcagg tgcaaacaat ctccaaaaca ataaagagtt
     2881 agttgacaag ttaaatgtat tccctgtacc agatggagat acaggaacta atatgtcatt
     2941 aacaatatct tatgccttaa aagaattagc aaaagttgaa aatgataata tatcagatat
     3001 cggaaaagca ttatctaaag gttctttaat gggagctaga ggaaactcag gagttatact
     3061 ttcacaaata atcagaggta tagctaaatc tatagaagga aaaagtaaat tatcaacaga
     3121 agatttagca aaagctttta aaaatggttc agacacagca tataaagctg taataaaacc
     3181 aattgaagga actatactta ctgtagttag agaaagtgga gagtttgcaa taaaaactgc
     3241 gaaaaaagag aaagatgtag taaagttttt aagtatgctt gtgaaggaat caaattcttc
     3301 attagaaaga actccagacc ttctaaagaa tctaaaagaa gctggtgtag ttgactctgg
     3361 tggaaagggt cttgtactta tatatgaagg aatgttagct tctataaaag gcgataacat
     3421 agaaataaaa aatgttgacc tagatactaa tatttcaaca agtatggatt ttgcaaagag
     3481 tactactagc acagatgata taaaatattg ctattgtaca gaatttattt tagaaagttc
     3541 taaggtagaa gatactaaaa taagagacat aatgatggct tatggtgata gtctagcagt
     3601 agttggagat gatggagtca taaaggtaca tgtacacact aatgacccag gaaatgtact
     3661 tcaagaagct ctaaagtacg gtcaattatt aactataaaa atagaaaata tgaagttaca
     3721 acatgaaaat acattactgg atgtagaaga aaagaaagaa aatgatagtg aacctttaga
     3781 agaagagaaa gagtttgggt ttatagctac ttctatgggt gaaggtttgg caaatatatt
     3841 taaagatttc ggagttgacc atataataga aggtggtcaa actatgaatc caagtactga
     3901 agattttatg aatgctataa agcatataaa tgctaaaaat atatttattt tcccaaataa
     3961 cagcaatata ataatggctg ctaatcaggc aaaagaattg agtgataaaa atataatagt
     4021 tatacctaca aaaaatacgc ctcaaggatt tgctgcatta gtaactttta atggagaatt
     4081 aagcgaagat gaaaataaag aagctatgat gaatgcttta aactcagtta aatcaggtca
     4141 agtaactttt gctgttaggg atacagtgat gaatgaaatt gatgttaaag aaggtaatat
     4201 aattggaatt gcagaaggaa atttattatc agcaggagat tatgtggatg aagtaacatc
     4261 aaaccttatt gaaaagttgg ttgatgaaga tactgctata ataacattat tctttggtga
     4321 agatgttaca gaatctcaag caaatgaact acgtacttct ttagaagaaa agtttgaaga
     4381 tgtagatgta gagttatatt atggaggaca acctctttat tattacttaa tttcagttga
     4441 ataatgatta taagaacctg tatgaccaag tagcttttat aaaattggtt gtacaggatt
     4501 tttgtgtagt aggtgatata tttgttggat ttatataaag atgttcaata tgttaaggga
     4561 ataggaccta aaaaagcaga taaattaaat aaactgggta tatttacact aaaagaccta
     4621 ttgtattatt ttccaagaca atttgaagat agaaataatc taaagaaaat agcacaatta
     4681 gaagatggtg agaaagttac gataaaagca gtaatatcaa gtataaatac atttagccca
     4741 aaagaaggta tgactttaac aaagattgat gtaaaagatg agacaggctc ggctaaatta
     4801 gtgtttttta ataaaagtta tataaaaaat acttttcgtc caggagattc aatattagtt
     4861 tttgggaagg ttaagaaaaa atttaataac ttagaactta catcatgtga attagaatat
     4921 cttactaatt cacctaagaa tacatgtaga tttatgcctg tgtaccagct tacatacggt
     4981 gttacaaata aagaaattat gagtataatt agaacagtac ttgaagataa agaattaatt
     5041 atacaagaat atatgccaca aagaattata gaaaaatata ggctatgtag tatagatttt
     5101 gcagttagaa acatacattc accaagtagt aaggaatcat taaaaatagc tttatataga
     5161 attgtatttg aagaattact tatactacaa ctagggttgt ttgtatttaa aagtggcaga
     5221 aataaagaag atggaataaa gtttgaaaca agtaaagact taaaaaaaat aataagtgct
     5281 ttaccattta aactgactaa agctcaaaac agggcattag atgaaattat acaagatatg
     5341 aatttagaga aaataatgaa tagattggta caaggtgatg tagggagtgg taaaacagta
     5401 gtagctctct tagcacttgc aaattgtgtt ttaaatggtt atcagggcgc tttgatggca
     5461 cctacagaga tattagcagg gcaacattac atttcactta ctgagtctct aaaagatttt
     5521 ggaataaatg taggtctatt aataggtagt cttacaaaaa aacaaaagga tacagtattg
     5581 gaacaaataa ggaataatga gattgatata ttaataggta cacatgcact aattgaagat
     5641 aaggttgaat ttaataatat tggtttagtt ataacagatg aacagcatag atttggtgtt
     5701 atgcaaagga gcaaactttc attgaaagga gcaaatcctg atatattagt aatgacagca
     5761 acaccaatac caagaacttt agcacttata ctctatggag atttagatat ttctattata
     5821 gatgaacttc ctccaggaag acagccaata gaaacaattg ctattgaaaa aagtaagcga
     5881 gatagagcat ataataacct tgtaagaaga gaggttgaat caggaagaca agtgtacatt
     5941 gtatgtcctt tggtagaaga aagtgaagct atagaagcta aatcagcagt tgaacttgta
     6001 gaggaattaa gagctgaata tttccacgat ttaaggcttg gattgcttca tggtaaaatg
     6061 aaatcgagtg aaaaagacga agtaatggaa cgttttaaaa ataaagaaat agatatttta
     6121 gtttcaacaa cagttataga agttggtgtc aatgtcccaa atgcaacatt aatgataata
     6181 gaaaatgcag aaagatttgg acttgcacaa ttacatcagc ttagagggcg agttggaaga
     6241 ggtagtcata aatcgtattg tgtactaatt tatgattcta aaacagatgt atgtagacaa
     6301 agaatggcta taatggaaga gaccaatgat ggttttaaga tttcagagaa ggatttagaa
     6361 ataagaggac ctggagaatt ttttgggaca aggcaacatg gtcttccaga acttaaagta
     6421 gcaaacttat ttaaacatat aaaaatattg aagttagcac aacaagaagc tcgatatata
     6481 ttgggtgaag ataataatct acaattgaaa gaaaacatgg cactcaaaaa agaaattata
     6541 gataaattta aagatacatt gaaagaaatt tcattaaatt aattttaaat tatgttaaaa
     6601 tattactaat gtaataatgt gctgaaggga gattgtcata ttgagagtaa tttcaggaaa
     6661 agcgagagga ttaaaactaa atactccaaa aaatgaagat gtaaggccaa ctacagatag
     6721 agtaaaagag tctctattta atatgataaa ttcatatata atggaaagtg aagttctgga
     6781 tttatttgct gggactggtt ctttaggaat agagtgctta tctaggggtg ccaagtcatg
     6841 tacttttgta gatatcagta aggaaagcat agatattgtt aagtccaatg taaaaaaggc
     6901 aagagttgaa agtgaaagta ttattttaaa ccttgatttt aagactgcaa tagataaact
     6961 taaattacaa aatagtaaat ttgacataat ttttatggac cctccgtatt ataaaaatat
     7021 gtttatagaa gctattgaaa aaattgataa ttctaatcta ttgaacgaag atggaataat
     7081 agttgtagaa catgatacga atgatttatt tccagataag atatgtaaat tggagaaaac
     7141 tagagataag aaatatggaa atactacatt aactttttat aagatggagg cataatatgg
     7201 aaaataaacc tagaaaagct atatttgcag gaagttttga cccaattact aatggacatt
     7261 tagatattat ttgcagggct tcgaagttat ttgacgaatt acaaatagga gtattaaata
     7321 atcctaataa aaagggtcta tttagctttg atgaaagagt aaaacttata gaaaaaagta
     7381 caagccattt aaataatata aaagttgtga cttttgatgg tttattaata aattactgtc
     7441 aggaaaatgg gattggagca ttagttagag gtgtgagaag tggtgctgat gttgattatg
     7501 aacttcagat ggcacatatg aatagagagc ttaatccaga tatagagact ataattttac
     7561 caagttgtac aaaatattca tttataagtt catctttaat aaaagaggtt ttgctctttg
     7621 atgctgatat aaaaaatttg gttccaaaga ttgttttgga agaattaaag aaaaaaacta
     7681 gtgggggtaa ttgatatatg aagatagatt tagaaatgat tgaactattt gagcagttag
     7741 aggagatatt tagaagtgct tctacaatcc ctttctcaca taaatgcaca gtggataaag
     7801 atgaggtttt agcaataatt aatgatataa gaactttaat accagaagaa gtaacacagg
     7861 cagtatggat taataaagaa agaaataaaa taataagtca agctaagcaa gatgctacaa
     7921 atatagttga acaagcgaaa aaagaagctg agagaataca acaagaatat caagataata
     7981 tagaggaatt aaagaaaaat tctgaagatg tagtaaaggc atatgtagaa tccagcgaac
     8041 ctgttgttca agcagaccaa agagcaaaag atatagttga tagagctgag agaatagcca
     8101 atgagataag aattgggtca attgaatatg cagaggatgt gctttctagt gttgagtata
     8161 atttaagaga aatattagaa gaagtaaaaa gagataaagc tgaacttaga cctaaaaaat
     8221 aaattttaaa taaataaaaa tggagctatc acacaaaaaa ttccaattga tgtgtgatag
     8281 ctctctttta atatagcaat tttttgttat ataattgtgc tttttagatt acttatatct
     8341 tatttt
//
LOCUS       DACTGW010000168         8267 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.168, whole genome shotgun sequence.
ACCESSION   DACTGW010000168 DACTGW010000000
VERSION     DACTGW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.168"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(609..1202)
                     /locus_tag="I9573_002808"
     CDS             complement(609..1202)
                     /locus_tag="I9573_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAT4797417.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
     gene            1763..1993
                     /locus_tag="I9573_002809"
     CDS             1763..1993
                     /locus_tag="I9573_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797418.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            2050..2700
                     /locus_tag="I9573_002810"
     CDS             2050..2700
                     /locus_tag="I9573_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAT4797419.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRMYFNRKNRIVTK"
     gene            2914..3585
                     /locus_tag="I9573_002811"
     CDS             2914..3585
                     /locus_tag="I9573_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4797420.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCITANDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            3582..4712
                     /locus_tag="I9573_002812"
     CDS             3582..4712
                     /locus_tag="I9573_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4797421.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDAISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLIINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RKGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            5117..5962
                     /locus_tag="I9573_002813"
     CDS             5117..5962
                     /locus_tag="I9573_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797422.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            5962..6990
                     /locus_tag="I9573_002814"
     CDS             5962..6990
                     /locus_tag="I9573_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797423.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGVKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            6993..8006
                     /locus_tag="I9573_002815"
     CDS             6993..8006
                     /locus_tag="I9573_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797424.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTML
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGAPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILVVHCVVWFMIYIILDKKRKLS
                     FSR"
BASE COUNT         3082 a          844 c         1363 g         2978 t
ORIGIN      
        1 taataaacta atttaggaag tatagaaaat taatctaaag aagtataata aactaattta
       61 agaaatatag aaaattaatt taaagaaata caatacatta atttttaagt aacattaaat
      121 taatgtaagt aaaataatgt tcatatgaag atttaaaggt gcattttctg aagctaagta
      181 tatgttatgt ctctatatcc ggtgaagttt aatagctcaa gaaaatgcac tatttatgat
      241 gattattttg ttttatttcc aattttggaa ataactattc taattaaatc taaaattata
      301 ccataaataa gaaaagaaag tatagatgct atgtaccaat aaatcgaaat tgaccctgta
      361 attgttctac atgggatttg catgcctgaa tccataaaaa caaaaaaact atcaatggta
      421 gaaataatag acaaaatagg atttaatcct attagaataa tactttttga gtcttgacca
      481 atttggtgga tataaatctc aaataatgca attaaaaaaa acaaaatgta aaagatttaa
      541 taattttttt catcaaaata cctcacctaa tataaattct gtttgcacta tttgcttact
      601 ataactattt atctccattc agtcacatca cttaattgta atctaggtct tcttgatggt
      661 tcttcgtcag gataacctaa agatattgct gccactaatt gactatctgt atttaaccat
      721 tcacataatt cacgatatgc aaagtataca tcacaaatcc aaagactacc tagacctaat
      781 tccacagctg taagcgacat attctgaatt gcagctccaa ttgattgcat gtttgccatt
      841 tcatagaatc gttcttcagg ggatagcttt tctaaaggtg atttacctaa tatatttaaa
      901 ataaaaattg ttactggagc ttgtttcatg atttcaactg tgtaatttgc accagcgata
      961 tgttgacgac ttcctggaag aagcccatta tcattaattt cattttgaat acctttgctc
     1021 atggctttaa gcatagattc tttttccttt tcagtgatga caacaaaacg ccatggttgc
     1081 ctgtttttag atgaaggtgc atttattcca gcttcaatta ttttctctat agtttcatgt
     1141 gaaatgcttt gatttttata ttttctgata cttcgtcttt ttgatataga atcacttatc
     1201 attttaagta acctccgttt attttttgga aaaatctatt tattttctat ttttaattat
     1261 tttagcatta atcagaaaaa aagtcattga ttttagtata caattattaa cgattatatt
     1321 aaggattgta tcaaaaagta tgtgattata ctaagggatt atattagaca ttatatttaa
     1381 aattgtatga aaggttgtat ttaatattat attaagagag atatatatta ggatttatat
     1441 atattaagat ttattatatt gagcccttat attaaattaa ggattataat ttaaaaataa
     1501 gctataaaaa cccaatattg ttttgttcag agataaaaaa gaatacttta ggtgattttt
     1561 attaaaaatt aagagtgata agattatata ctttataaat gtaaatatga cgaataaaaa
     1621 tgataaatat aataatctat atatttacat taggagtttt ctatactata attatttata
     1681 tttaaaacta aatataaatg ttggaattat ttaggatttg aaattgtcta aagataaata
     1741 taagtgatga agaggtgatg tgatgagatt agatatggat agtatgttat atgtactaat
     1801 ttatatgaca ttattggtat ctatcattgg aagtatattt tatttttgta aagacctctt
     1861 aaatcataaa attaagtatg ttttaataag agacatattt gtaaatagaa ttacatattc
     1921 tgaagaacgg ttgcctaaac agtatatagt ttttcagaaa tatgatattt ggaggtattg
     1981 tagtttgttt taatttgaaa tgaatctatt ataaattcta aagtttaaaa ataaaaagga
     2041 gagaataaaa tggatttcat ttcaagtata ttgcaaggat tattaagtat attatttaat
     2101 tttacaggtg attttggaat ttctattgta cttataactt taatagttaa attagtactt
     2161 atgccactat cattaaaaca aagattttct gtaaaaaaac agcaagaatt agctgaaaaa
     2221 atggaacata taaaagaaaa gtataagaat gatactaaag aattggaaaa gcaaatacaa
     2281 gtaagttctg tagaaagtat gaagagtatg ctgggatgtc ttatagtttt ggttcaaatg
     2341 ccagtagtat atgctttata tcatgtatgt ttagatatga caaaggaatt tacaacaatt
     2401 ataattccat ggattgctaa tttaggtaca tttgataatt tgtttatatt accttgtata
     2461 tatacattaa ttatgttagc ttcaaattta gtgaattata taccatattt aaaaataaat
     2521 tctcaagtaa agtttaacaa acaaatggct gtggtgacta ccataacaag tcttatacta
     2581 actgttagaa ctccagtagc cataggtctg tactttataa caagtgcaat ttattcatta
     2641 attgaagata tttgttttag aatgtatttt aatcgaaaaa atagaatagt gacaaaataa
     2701 tataattatt attagaaata ttttagcctg ctaacataga aaaagttagt aggctatttt
     2761 tatctatgat atcaattcca ttaagaatag tctaaaaaat actatcaaaa tgctataaaa
     2821 aatactataa aaatactata aaaaaatagt tgaaaaataa ataaattaat ataaactaaa
     2881 ataaagccta caaaaaagag gtgataaata gaaatggcta aaatactagt tgtagaagat
     2941 gaaaaaagga tgcaaagtat tattgtagaa tatatgcaaa agggaggata tacttgtata
     3001 actgctaatg atggagtcga ggcacttaca atattaaaga gcaacaatat agatttaatg
     3061 atattggata ttatgatgcc gtatttagat ggattttcag tgtgtagagt ttcacgtgaa
     3121 atgactaata tacctattat catattgaca gctaaaggtg aagaggagga caaattaaag
     3181 ggttatgaat atggcgcaga tgattatata acaaaaccat ttagcccaaa agtactttta
     3241 gcaaaagtta atgccttgtt acgtagatac actactgata ttcccaaaaa ttcattgagt
     3301 ttaggaaaga tttttattat ggtagcatca aggcaagtgt atgtagaaga taaattgatt
     3361 gatttaacat ataaagaatt tgaattattg cgtttattta tggaaaaccc taatcaagtt
     3421 ttttccagag agaagctttt aaattgtata tggggatatg attttgaagg aaacacacga
     3481 actgtagata cacatataaa aaccttacgc aaaaagcttg gtagtgaagg acaccatata
     3541 gttacattaa ttcgttcagg atataagttt gaggttaagg aatgaatagt ttaagaaaga
     3601 ttatagaaaa actgaaaaat gtagatattt caaattggat taaacaaaat tttggactat
     3661 attcagttag aaaaaaagtg tttttacttt ctaaattatc aggagcgtta attatattat
     3721 tttatctggt tgttgaagaa ttttcgataa actctaaatt tgaattttgg atttggttta
     3781 taatactggt tatatttatt atgagtattg attttttatt gggaaaattt atttcagaac
     3841 caatcacatc tataaataaa tctgcaaaaa gtatgtccca acttgatttt tctaatccat
     3901 gtactgtaaa tactaatgat gaatttggag aactttctag gagcctgaat acaatgtcta
     3961 caaatctaca acaggcatta tcagacctag aaagtgcaaa tattcaactt gaaaaggatg
     4021 taaataagga aagaatgcta ttggaacaaa gaaaggagtt ggtagatgca atttcacatg
     4081 aaatgaagac tccacttggc attatacgtg cgtatacaga ggggcttatg gatgaagttg
     4141 atgaagaaaa aaagaaaaat tatatgaatg ttataataga agaaactgat agaatgaaca
     4201 atatgattgt atcattatta gacttatctg cattggaagc aggagtttct aaacttaacc
     4261 cagagcgttt tgattttata gagtttgttg aaactgtggc aggtcgtctt ttaattgata
     4321 taccagatgc caatttttat tttacatatg atttgccaga gtatgaagtt tttgtagtgg
     4381 cagataaaat gagaatggaa caagtagtag agaatctaat tattaatgca aagaagcatg
     4441 ttgtatataa tggtaattta gatttatcga ttacatgtga aaatggcttg cttttatttg
     4501 aaatttataa tgatggtagg attatagagc aagatgagat ttcaaagatt tggtctaagt
     4561 tttatcgtag tgttcaatct cagcgtaaag gaggttcagg tctagggctt gcaattgttt
     4621 cacaaatact tacaatgcaa gggttagaat atggagtgaa taatcgtgat agaggagtag
     4681 agttttattt tatgattcca attgatgaat aatttagtat taatttataa attataagta
     4741 tctgatgtaa aatatttttc tttgtatacg taagtattta aagagaataa aataaaaaac
     4801 ttaatgaaaa agtctaataa aaagtttaat gaaaagtctt gttttcctga tgaaaacaag
     4861 attttttatt aagcttttta ttatttcact tcgatttcac aagagagaca ctctgatttc
     4921 accaaataga aataaaatag gttaaataaa ttaaaaaata gaagcgtaat taaataaggt
     4981 tacaaggggg agtacttatg aaatggtata catggttgac tattatattg ataatattgc
     5041 tttctattcc gcttaaaatt aaatttataa aatggtggga tagacgacaa aaaaatgata
     5101 aaggaggtta tgatagatga taaaagtagg agagttaaaa ttttcttatg gaaaagataa
     5161 acagatattg catggactta attttgaagt aaaagaagga gaaatctttg gattccttgg
     5221 acctaatggt tcaggaaaat ccacaacaca gaaaatctta aatggcgttt taaaaggtta
     5281 caatgggcac gtatcccttt ttgggaagga agttaaagca tatacagagt ctctatatca
     5341 aaagattggt gttttatttg aatttcctta tttatatacc aatttaagtg caattgataa
     5401 tttagaatat ttttcgtctt tttatcctaa aaatcaaagg agagacatat atgaactctt
     5461 agatttgtta gagtttaaga aagagtttat aaataagcct gtatcatcct actcaaaagg
     5521 tatgaagcaa cgtattagca tggcaagagc attggtaagt aatccaaagt tgttattttt
     5581 agatgagcct actagtggac ttgacccatc tggagctgta ctatttcgta agattattga
     5641 agaagaacga aaaaaaggaa caactatctt tttgactact cataatatgc tagatgcaga
     5701 tttaatgtgt aacaatgtgg cttttattgc agatggcaag attatggtaa tagataaacc
     5761 caaaaattta aaaatgaaaa atagcaataa taaagttgaa gtggaatttg tatataatgg
     5821 aaatcgagat attgaaattg tagatataca agagttagaa tctggaataa cttttaaata
     5881 tgatgaaatc ttaagtgtac attcaaaaga acctacacta gaagaagttt ttattaaata
     5941 cacaggaagg atgttagtgt aatgtattct aaatttttta ccttagtaaa aaaagatttt
     6001 tggatgatga tttatggtaa attttttgtg ctgacacttg gctctctcat actttattcg
     6061 tgttatatta attttggtta tgtaaagttt atggatgaag atagttatca caataatgta
     6121 tatctatata atccagagaa tataagcatg agtgcatctc cattaattaa tcaagtttct
     6181 tctataggtg aattaaaagc aaaacttgat gaagatacaa gtgcagtttg tattgatatg
     6241 agttccagta agccgaaagt aattttacat gaaagtatta aaggagttga caattataga
     6301 gctgactatg cattgtcatt attatcatca gaaaaagacc ataatagcaa aatatcagac
     6361 aacaatgcaa taattcaaat taatgataag gatacatctg agataaaaca aagaaaagac
     6421 atgacttgtg agattttatt ctttgaaatt gtagctattg gatttttagg gattgcctct
     6481 ttattattta aagaaaagca gatgggagta atgcgtatta ctggtatttt acctgtaaaa
     6541 agaagtcttt ttatattatc aaagatattt gcttttttga tatgtgacct atgttttaca
     6601 gttcttttag tagtatttaa tattggattt tttgaaggag taaaaatact tccacaagta
     6661 ttagttcaag ctagtatttt atcaattatt atgtctttga ttggaatagg atgctcaatg
     6721 ttattgagag attttaagca attttctctt atatatcttg tggttgctgt attcataact
     6781 acacctatat ttctatctgc aaatacatct tttgaaatag attggattaa atatcatccc
     6841 ttctatcatg tttatagtca gcttaaaaat gcgtttttca tgaatttttc aaaagaatta
     6901 gtttattttg tagtgtgttc tttgacaata atagttctat ttttacttgt aaaatatatt
     6961 tttaataaag agttggttag ggagggataa tcttgatagg attacgttac cagttaaaga
     7021 gtataaaaaa agataaaatg tgtataattt ccttcttttt accagttgtt atggcgattt
     7081 taataaattt tatgggaaca attgatatat cttcattagg agagatacgt tttggtatag
     7141 tgaaggatac acttcctgta caaacagagc agtggttgaa gaagtatggt atggtaagtg
     7201 tatatgacac aaaaatgcat ttaatacgtg aaattaagga tattaaaacg gacacaattg
     7261 gagttgttag agatggtaaa ggaataaaaa ctatgcttta tggaaatgag attaatgcta
     7321 taaaaaatgt tggaaagaca ttgccagaga tgtttagact aaagggtgat ttaaaagatg
     7381 ttgaggttac aatattgcct aaagatagta ttttaaaaga gtttcagaat gtttttattg
     7441 ctatgatttt aattactgct atgtttatgg gatgtacctt taatgcaatg aatattatat
     7501 ctgaaaaaga agatggaata tcatttataa atgaagtttt gccacagagt agaagtcagt
     7561 atattataca aaaagtattt ttgggtcttt tatgtgggtg tttatcttca atgataactg
     7621 tatttatatg tttggatatt tcaatattag atatgataat gatgttgtta ctcgttgtat
     7681 tttcagcatt tatttcatca cttataggtt tatttattgg aaagttgtca gaaggtctta
     7741 tggtaggaat tgtttatatt aaaatagtaa tgattttatt tataggtgct cctttgttaa
     7801 tatatcttct aggggttaat accagcggta tagggaattt atgctatata atgccatcag
     7861 ttgctacatt taatggtatt atggagatta ttaatggtaa tgttattatt ataaaagata
     7921 ttgtaatttt ggttgtacat tgtgtagtat ggtttatgat atatattatt ttagataaaa
     7981 aaagaaagtt atcttttagt agataactca gtataattat aattgggaca atttaatagg
     8041 tgatttaata tactttgtat agtgtaattt atgtagtgca atttgtatac tttaatttat
     8101 gtagtgtaat agttagtttt attttataat tattcaatat aatttagtaa gaaattcagt
     8161 acaattagag ctacttagtg gaaaacttaa taggtactta aactatataa atactttatt
     8221 ttcctccatt tgtactataa atttaattta tgatataaat ggaggta
//
LOCUS       DACTGW010000169         8129 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.169, whole genome shotgun sequence.
ACCESSION   DACTGW010000169 DACTGW010000000
VERSION     DACTGW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.169"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            150..1382
                     /locus_tag="I9573_002816"
     CDS             150..1382
                     /locus_tag="I9573_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HAT4797425.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            1396..2202
                     /locus_tag="I9573_002817"
     CDS             1396..2202
                     /locus_tag="I9573_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HAT4797426.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKHGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2314..3183)
                     /locus_tag="I9573_002818"
     CDS             complement(2314..3183)
                     /locus_tag="I9573_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAT4797427.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3554..4981
                     /locus_tag="I9573_002819"
     CDS             3554..4981
                     /locus_tag="I9573_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HAT4797428.1"
                     /translation="MKLTKSISSMLCLALVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFLNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEIFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5057..5326)
                     /locus_tag="I9573_002820"
     CDS             complement(5057..5326)
                     /locus_tag="I9573_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797429.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGISINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5329..6771)
                     /gene="nhaC"
                     /locus_tag="I9573_002821"
     CDS             complement(5329..6771)
                     /gene="nhaC"
                     /locus_tag="I9573_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAT4797430.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAATKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDILNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(6796..7962)
                     /locus_tag="I9573_002822"
     CDS             complement(6796..7962)
                     /locus_tag="I9573_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAT4797431.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESVLVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIK"
BASE COUNT         2980 a         1065 c         1292 g         2792 t
ORIGIN      
        1 cttaaattat atcaaattga taacaaacta gatattatac ttttttgtag aatagaatta
       61 caattatata aagaaaaatt ctattaatag tgaaaaaaaa gtagaaaaaa gtagaaatta
      121 ttcttttata aagaaaggga gggataatat tgcaagagag agaagaatta gttttaaatc
      181 cagttccaaa agaggaaaga gttggatggt tagctccatt gtttaatatg cttggttcta
      241 acatagctat atctgaacta atggttggag gaactttaat tttaggaatg actctttcta
      301 atatgattat tacatcaatt atagggaact taatattagt tgcaatcatt atgatacaag
      361 gttatatagg ttacaaagaa gggttaaata cgtatgtatt agctaagggg gcatttggag
      421 agataggagg aaagtatctt atatctctat tacttggtat aactagtttt ggatggtttg
      481 gtgtacaagc aggggttgca ggactatcag tacaaaagat attcccaagt gtaaacctaa
      541 cattagttac agttatactt ggattattta tggttgtttt tgcactgtat ggctttaaag
      601 ctatggctaa atttaattac ttagttatac caccacttat gatattaatg gtgtggggag
      661 tttttaaagc tttttctact tatggagttg aagctattta taattatact cctcaaacaa
      721 ctatgagtat ggttgaaggt cttaatattg tagtaggtct tgttatagtt ggtgcaatta
      781 tatcacctga ccagttaaga tatacgagac gagtaaaaga tatttggatt atcagttttt
      841 taggtctagg gattatttca ttattccaac aagttgcagc tggtgtaatg tctatgggag
      901 cacctacttg ggatataaca gaagtattgg ctaatttagg ttttggatgg gtagcgtttg
      961 ttattttaat acttgcatct tggtcaacaa acgtatcaaa tgcttattct ggtggattag
     1021 cattaaaaac tatttttcca aatgtaaaaa gaaatgtttt gactttagtt gcaggtctaa
     1081 taggtacaat aatagcagct actggtataa tatttaaatt tcagtcattt ttatctttct
     1141 tgggaatcgc agttcctgca atagcaggaa ttatgtggtg tgagtattat tttatacaag
     1201 gaagaactta taagcataga gaaggaataa attggattgc agtaataagc tggttaatag
     1261 gttttgcagc gtcgtattat agttcaaaaa taaatttctt aatacctccg ataaatggaa
     1321 ttgtagtgag tatggttatt tattatatat taatgaaatg ttttggaatc aaagataaat
     1381 aaagggggca attacatggg tcgtaaggtg aaaataggta taattcaaca acacagtgta
     1441 cttggagatg ttaaaaaaaa tatagaaaaa gcagtagaga tgatagatga cttaggaaaa
     1501 catggggcag atataatttg tttaccagaa ctatttgcta caggatataa tttagagtct
     1561 ttaggtggag ttaaaacatt agaattaata agagaacata ataagtacat agaggaatca
     1621 atgtcagaag ctgctaaaag aaataatgta tacctgatat ctccatatgg aaccttagaa
     1681 aaagggtcta cacatgttta taattcagca gttatatttg atagaaaagg taagattatg
     1741 ggagaatact gtaaaaatca tctgtggtca ttagaagcag tatactttaa aggaggggaa
     1801 aaagtagagg tatatgatgc tgattttggt agatttggtg taatgatatg ttatgatgca
     1861 ggttttccag aagtatccag agaattgaca ttaaaaggtt ctgaaataat atttatacca
     1921 tctgcatgga gaatacaaga tgaagatatg tgggatttaa atgtatctca aagagcttta
     1981 gagaacactg tatatacagt aggtgtaaat ttagtaagta atgactctaa tttaatacta
     2041 tttggaaaga gtaaaatatg caatcctaga ggtactgtaa taactcaatt agatacatac
     2101 agggaaaaat atgtattatc agagattgat ttagatgaaa taaaaaagta tagggaagat
     2161 ataccctatt tgaaagacag aaaaagtagc tataacatat aattctatac tataatacaa
     2221 gttcaatata aaatgataaa aagaggatgt atcaatatta agattaaatt tattatattt
     2281 tgatatatcc tctttaatat tatcaacttt gatttaaata tttttaaaat agttgataag
     2341 aaagtttttg aacaacaaca cagctttaga ttcatagttt tcgctaaacc atgaaagatt
     2401 taaatatctt ttaaattcaa tatctttgat atgtaacaac ttaatctcag ggtcttgaga
     2461 taatccccat gattttccac atataaatcc aactccttga agtgatttta taagagcata
     2521 aattgtatat ggtgaatcag actcaaatgc tattttgggt ttgaaattag ctgattcgca
     2581 caggatatca attacctctc tataattctc acccttcgtt ataactataa agttttcatc
     2641 tgaaacttcg cttagatata cttcatcctt taatgataaa ggatggttta ttgaaactcc
     2701 taatagtatt tcttcgcatg ttaaagttat gctattttct gagtttagct ttgtaaatga
     2761 agaagatatg tacaaatcaa aatcactttt tttataagat gatggcaaag tatgtgacac
     2821 attaaaagta atattgggat atagttttct gaaattactc aaaagtgatg gtaacacatt
     2881 agccgctgca ggagataata tctttacttc tcctgaattt tctaaagaag tgtcttgtaa
     2941 ctcctttttt gaactttcta ctaaatctaa aatgctatct actcttttta gaaacaatct
     3001 tccatactca tttaaaacaa tatatcttcc ttttctgtca aataactgaa ctccaagttc
     3061 tttttctaag gaagatatag ttttgcttaa agctggctgt gctatattta acttgtgtga
     3121 tgcttttgtt atgtgctcca ttctagcaac agtttgaaaa tacttaagat gcaataaatc
     3181 catagtataa aatacacctc ttttaatatg acttgagtta tatcaatttt ataatcaact
     3241 ccacattata cattataata taaagtttat ataatcaact acaatatatt aaaaagttat
     3301 ttaaattgtt atttatattt tgtacataat ctaattaaaa aaagtagctt taaatgatta
     3361 actaaggaaa tgttgttaca aactttagtt atataaaaaa ttcatttgat gagtaaagta
     3421 ttaatgtcaa gtaagaataa aagcttatta gatagaaaaa taatatttta taataatgat
     3481 gttacttaaa ttattatgtt atactatttt aaataggaaa catttagata ctataatttt
     3541 agggggagtc aagatgaaat taactaaatc aatatcatca atgctatgct tagctctagt
     3601 tttaagctta tcaccagtag catatgcaaa tgaaataagt agaacaacag tggagaatac
     3661 tttagtaggt caggacagat accaaactgc tgttgaagta agcaaaaaag gatggagtag
     3721 tgcgaatgag gctgtagttg taaatagtga atctattgta gatgcattag ctgtaacacc
     3781 atttgctaaa ttaaaaaatg ctcctatatt attgacagaa aaaaataatc taaattcaca
     3841 aactgagaaa gaattaaaaa gattaggagt taaaaaagtt tatataattg gtctatctag
     3901 ttctgtatca aatgatgtgc aaaaacagtt agaatctaat aatttaacta ctgatagagt
     3961 tggtggtagt gatagatacc aaacagcttt gagtatagct aaaaaaatag aaggattaaa
     4021 agatatatca gaaatagctg tagtaaatgg gtacacaggt cttgcagatg cagttagtat
     4081 agcttctgta gctgctacta atggaatggt tatacttcca gtatctgatg catctggtat
     4141 atctagcttt aaagatttta tagaatctaa gaatataaat aaatcataca taataggctc
     4201 tactaatgct gtatcagata aaataaaaca aagtttacct aattcagaaa gaataggtgg
     4261 agcagataga aatgaaacta atggtaaagt aatagaaaag ttttatacat ctaataaatt
     4321 aaacaatgta tttgtggcta aggatggaat gaaaaatcaa aatcaactta tagacgctct
     4381 agcagtagga gtactggcat caaaagaaaa ttctcctgta ctaatcgtag gagaacaatt
     4441 aagtgcaaca caaaagagtg tatttttgaa taaaaatgct acctctataa ctcaagttgg
     4501 tagtggagga aatgaaaatg catttaaaga attaaaagat ttacaggcaa atagtaacaa
     4561 taagagagta atgtatgtaa ctaatacaga taaggtaaat ataagaaatg atgctacaat
     4621 agaagcttct gttataggat atctaaataa tggtgatgaa gtagaagttt tagatgtgtt
     4681 aaaaactgga tgggtaaaaa taaaatacaa tgaaggtatt ggatatgtta gtgggtctta
     4741 tttgacaaat aataagcctg ataatagtaa tgaaaatata aaaataaagt atgtaaaaga
     4801 aaaagatggt ctaaatgtta ggaaaggacc aagtacagaa gatgaaaaaa taggtcacct
     4861 ttcatatgga agcaaagtag aaacaataga aatatttgct actggatggg taaaaataaa
     4921 gtataatggt ggatatggtt atgtaagtaa tgattactta tctgatacac cagttattta
     4981 attataagta acaataaaaa actgctttaa tttgtaaagc agttttttta taaaaattta
     5041 agtctaataa ctatttctat tttgatacct taatcctggg atttattatt atggatatta
     5101 tcaacataca aacactccaa atccagtgtc taattccaaa cttagatatt aataaattca
     5161 gtcctgctat ggtaaataag cagtatttaa ttacaattaa aagtgtattt atagaaatcc
     5221 ccttcataga aagtatccta aatccaataa tagacattct aagtcctata aatgccaata
     5281 atatacctgc aatatcctga attaagtaat agtattcaaa tttcatattc aatcaactct
     5341 tctctgagca gtatcaaata tttctaactc ttcaatttca tcatctgtgt atgtaagcat
     5401 agtaaaacca gttacaccat aaataaatga aattataggt gtaatccaac ttaagaaaca
     5461 atatggaata aattctagtg ggcttacacc aagagtctga gcacagaaaa cagcatttga
     5521 attccatggt attaatggac caccaagtgt agctgtatct tctataattc tagatagatt
     5581 ttgtggtttt aatttaaact ctttgtatat tggagacatt agagttccga caaatacaga
     5641 agtaaacatc attgatgaac taaatgcatt tgcaaagtag cttactataa aagtagaccc
     5701 aaccaattta gttcttgatt ttcctatttt agttataaga ggttgaagca gtacttctaa
     5761 gaatccagtt ttttggagaa taccggcaat aacgaaaact acaaatacta gtaaaacggt
     5821 ttcagccata ctcattattc caccacgatt taatagtttg tcggcataga caaatccagt
     5881 atctatagta aatcctgata gcatgtaatt taaaatatca cctatttttt ctccttcttg
     5941 aaatactgct ataataagtc ccatgatagc tgaacttagt atagatataa tgggtggctt
     6001 ttgtagcaat aaaagtaata ataaaaatat tattggaatc attgctacta aaccaatatg
     6061 aaagtttgaa tttaatacat ccttgatttg atttatttga acatagtcta tagtattatt
     6121 tgaatactta aatccaatta ctgtatacag aactatacat attacgtatg caggtcctgt
     6181 ggtataaaac atatgcttca tatgtgtaat tacatctgtc ttacaaactg ctgcagctaa
     6241 gtttgtagaa tctgatagag gtgacatctt atctccaaag aaagcaccag aaatgactgc
     6301 accagctgtt aaaccaagag gaatacccat actagtacca atactcatca ttgctatacc
     6361 agaagttcca agagtccccc aagatgtacc agtagcaaca gaagttaatg aacatacgat
     6421 taatgttgta agcagaaaat atttaggggt tattattgtt agtccactat atactactgt
     6481 tggtattgta ccagcagcta tccaagctgc tattagtaca ccaacagaaa gtataattaa
     6541 gtttgattga aaggcatctt taccaacttc aaatccaaaa gcttctattt catcattagt
     6601 ataaccaagt ttcgtggctg caggaactac tataagccat gaaagtaaaa acattgtagt
     6661 tatagaagca ttaaatacaa ctataccaac tgatgttaga gctattattg caagcattac
     6721 taacaaggaa tatctaaatg aaggtatttt tctttctgta ttcattttca tagtgcaaat
     6781 tctccttaaa tatttttatt taattgcatt ttttattctt ataagagctt cttcaagcat
     6841 acttcttgga caagctaagt ttattctttg atacccactt cctccaatgc caaagctgtt
     6901 tccttgattt agagcaactt tacctttttg tacaagtaca ctttctagtt cctcatcact
     6961 taatccaagt gcactaaagt ctacccataa taaataagta ccttctggtt ttctaacttt
     7021 aagttttggc atattttcat ttatatattt tatagcaaaa tctatattag attctaaata
     7081 ttctaaaaat gattctagcc aagattcacc attattatat gaagcttcag ttgcaactaa
     7141 gctaaagcaa ttatttctct ttatatcaat tctggtaaaa gcatcatcta acaatttata
     7201 gtctttttca tcaggtagta ttacatatga tgattgaaga ccagctatat taaaagtttt
     7261 agttggagcc atacaagtaa ttgtattttt ttcaaattct ttagaaatag atgccatagg
     7321 aatatgctta tgttttttta atataatgtc actatgtatt tcatcagata tgatttttac
     7381 attatgctta agacaaatat ctcctagttt ttttaattca tcttttgtcc atactctacc
     7441 aactggatta tgaggattac aaagtatgaa taattttaca tctttaatct tgttttcaat
     7501 gtcttcataa tccattatat agtttccatt ttctagtttc tgtaatgggc ttataattag
     7561 ttctctattg ttatttttta caacactatt aaaagggctg taaactggtt cttgtatcat
     7621 tattttatca tttgcttttg tgagttcatt tattaaaaga cttatagctg ggataacacc
     7681 tggactataa attaaccact cactttttat tttccagtta tgtctcctat ctagccagtt
     7741 tactatagat tcattgtaag aatctggtct tgttgtgtag ccataaatct cttgttctag
     7801 tctatttttt aaggaatcaa ttatacaagg tgcagcctta aaatccatgt cagccaccca
     7861 cataggaagt aaatcattag ttccatactt tttttccatt tctgaccatt tagatgaaaa
     7921 attattgctt ctatcaacaa tctcattaaa attataattc attgtaaata cctccagtaa
     7981 ttttaatttt atgttaataa tattaagcaa acaacgtgcc aatattgata ataaaaaata
     8041 tttattcaca agttaatatt taatattaag taatttcaga gcatagataa gataaaaata
     8101 ttaattatac ttaaaacttt gtggaaaag
//
LOCUS       DACTGW010000170         8076 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.170, whole genome shotgun sequence.
ACCESSION   DACTGW010000170 DACTGW010000000
VERSION     DACTGW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.170"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(18..1658)
                     /gene="bclA2"
                     /locus_tag="I9573_002823"
     CDS             complement(18..1658)
                     /gene="bclA2"
                     /locus_tag="I9573_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HAT4797432.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGATGATGA
                     NGITGPTGNKGATGANGITGPTGATGATGANGITGPTGNTGATGANGATGLTGATGAT
                     GANGITGPTGATGATGANGVTGATGPTGNTGATGPTGSIGATGANGVTGATGPIGATG
                     PTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGLVGPTGATGATGV
                     AGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGATGANGVAGPIGPT
                     GPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGPIGPTGPTGANGTTGATG
                     ATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLGNNTLVTFNSSFINGTNITFP
                     TSSTINLAVGGIYNVSFGIRAILSLAGFMSITTNFNGVAQNNFIAKAVNTLTSSDVSV
                     SLSFLVDARAAAVTLSFTFGSGTTGTSPAGYVSVYRIQ"
     gene            complement(2036..2575)
                     /gene="hpt"
                     /locus_tag="I9573_002824"
     CDS             complement(2036..2575)
                     /gene="hpt"
                     /locus_tag="I9573_002824"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAT4797433.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2903..4210)
                     /locus_tag="I9573_002825"
     CDS             complement(2903..4210)
                     /locus_tag="I9573_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAT4797434.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDEKLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4635..6410)
                     /locus_tag="I9573_002826"
     CDS             complement(4635..6410)
                     /locus_tag="I9573_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4797435.1"
                     /translation="MMVMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDVCRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6622..7191)
                     /locus_tag="I9573_002827"
     CDS             complement(6622..7191)
                     /locus_tag="I9573_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAT4797436.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7375..8010)
                     /locus_tag="I9573_002828"
     CDS             complement(7375..8010)
                     /locus_tag="I9573_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAT4797437.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2294 a         1637 c          942 g         3203 t
ORIGIN      
        1 tcttttcatc atataaacta ttgtattcta taaactgata catacccagc tggagaagta
       61 cctgtcgtgc ctgaaccaaa tgtaaagctt aaagtaacag ctgctgctct agcatcaact
      121 aaaaagctta aacttacact tacatctgat gaagtaagcg tatttactgc ttttgcaata
      181 aagttatttt gggctactcc attaaagtta gtagtaattg acataaatcc tgcaagtgaa
      241 agtatggcac gtataccgaa agatacattg tatatccctc caactgcaag atttatagta
      301 ctacttgttg gaaaagttat attagttcca tttataaatg atgaattaaa tgtcactaat
      361 gtattattcc ctaaacttga agaagatact gtaaattgtg cattgtttgc tgctaacact
      421 cctgttgctc cggtcgctcc tgttggacct gttgctccat ttgctcccgt tgctccggtc
      481 gcccctgttg ctccggtcgt tccatttgct cctgttggtc ctgttggacc tattggacct
      541 gctactccat ttgctcccgt tgctcctact gctcctgttg ggcctgttgc tccatttgcc
      601 cctgttgctc cggtcgctcc tgttgctcct gctactccat ttgctccggt tggacctgtt
      661 ggacctattg gacctgctac tccattcgct ccagttgctc ctactgctcc cgttgggcct
      721 gttgctccat ttgcccctgt tgctccggtt gctccggttg gacctactgc tccatctgct
      781 cccgttgggc ctgtcgctcc tactgctccg gttggaccta ttgccccagc tactcctgtt
      841 gctccggtcg ctcctgttgg acctaccaaa ccatttgctc cggttgctcc ggttgggcct
      901 gttggaccta ccaaaccatt tgctccggtt gctccggttg ggcctgttgg gcctgttgga
      961 cctaccaaac catctggacc tgttgctcct actgctccgg ttggacctgt tgctcctatt
     1021 ggacctgtgg cacctgttac tccatttgct cccgttgctc ctatacttcc ggttggacct
     1081 gttgctcctg tatttcctgt tgggcctgta gcacctgtta ctccatttgc tcccgttgct
     1141 cctgttgctc ctgttggacc tgttatccca ttcgctcccg ttgccccagt tgctccggtt
     1201 agtccggttg caccatttgc tcccgttgct cctgtatttc ctgttggacc tgttattcca
     1261 tttgctcccg ttgctcctgt tgctcctgtt ggacctgtta tcccattcgc tcccgttgct
     1321 cccttgtttc cggttggacc tgttattcca tttgctcccg ttgctcctgt tgctcctgtt
     1381 ggacctgtta ttccatttgc tccagtcgct cctgtatttc ctgttggtcc tgtagaacct
     1441 gttgttccat ttgctccagt cgctcccata tttcctgttg gtcctgttat tccattcgcc
     1501 ccggttgctc ccgtatttcc tgttggtcct gttattccat ttgctccggt cgctccggtt
     1561 gcgcctctag gccccgtcgg tcctgttgga ccagtagcac ctgttggccc tgttggacct
     1621 acatcttgat ataaacttgg acctgaaata tcactcatat aattaatcct ccttttttaa
     1681 agttagagta ttactctata aatattgagt attactctat ataattatat gaatcgacaa
     1741 tttcttatgt gataatgtat ctatttgctt atatcaacat atatgctttg attcttaaat
     1801 ttcattactt ttttgtaaaa tcttttacaa ttattagtac tcttttacaa ttattaatac
     1861 aattaaaaaa ctcatttccc atcaattatt taaaattttt ttattatgtt ataaaaaata
     1921 gataattaaa ttgcataaaa ttaatttaat actactttaa ttacttttta aataaaaaaa
     1981 cctcaacacg attaataaaa atcatattaa ggcatccaca aaattatata attgcttatc
     2041 tatcttcatc agtaacattg aatatataag gaacatttct ataataatca ccaaaatcaa
     2101 acccatatcc aaccacaaac ttatcttcta tttcaaatcc acaataatct ggcactaaat
     2161 caacttttct tctgcttggt ttatccaaaa gaacacagca ttttaaacta gcaggatttt
     2221 tagcttttag atgttttaac acaaaatcca tagtaagtgc agaatcagta atatcgtcta
     2281 caaccaaaac atcatatcct gctatatcag tagtcacatc tgaaaccact ttaacctttc
     2341 cagtactttc ttcattgttt ccataactag atgtagtcat aaaattaact ctaagtggta
     2401 aatttatatt tctaactaag tctgcacaaa atataaagct accttttaat agagaaacta
     2461 ccataagatt tttatcttta taatcttttt ctataattgc tcctaattcc cttaatttat
     2521 ctttaatttt ttcttctgaa tacaatactt cccatttttt tgtttctata tccataaaaa
     2581 gacctccaat tgtaataatc cttcttaatt ttataagaga ttgctcactt tttcaatact
     2641 cttattcaat attagtcaat attattgagt ttttatgttc attcaacaat tattaacaat
     2701 gtatcttata ttttaagtag ttcatatatc cacactaaat tacatactat atattttaga
     2761 caaaaaaaga aaaaacttgc actaacaagt tttttctatt atacataaat ttatacataa
     2821 tcatatttta ttagagggta ctaattaaat ttatttatgc tatttttgtt atacctcata
     2881 atacaaaaca tattataaag tactagtcaa ctttattcat atcattcatt acatttaaga
     2941 ttatctcatc tgttatagac atacctttgt cacttactct acctaagttt tgtatacttt
     3001 gctcaacact tgaaccaact ataccattta gtggtggtac aaaacaatct tgcttagcta
     3061 ttatagcact ttgtatagct gcagatgctg cagaagataa ttttaatgca caaccagctt
     3121 tcgcaccgtc acatatcatt ccacttaagt cagctatcat attttctata gctccttcta
     3181 tttgcttttc agtaccattc ataagccaag agataccagc agttgctcca gtagaagcag
     3241 caacaccaca tccacacaca gcagataatc ttcctgtata gttcttaata taaccagtta
     3301 ctagatgaga tatagctaaa gcctttgcta gtttttcatc agattgtggg aattttttat
     3361 tataagcaac gattggaaga attgctgtta atccatgatt tccacttcca tttgaactca
     3421 taacaggcat ttttacgcct gccattcttg catctgaagc tccagcagtt aacatcattg
     3481 cataatttat aacatcatta cctaaaagac cttcttctat agacttttta attccatatc
     3541 cgacaccaat accagttttt tgttttaatc catattcagc catctcttca ttcattttta
     3601 ctccatctag taaaaattct atatctttaa aatcaatttc ttctacattt tttattaatt
     3661 cttgtatagt tactgtatcc attaaacttt cagctttttc tgccgcatca tttgaagctg
     3721 atacttttgg ttcattgtct aataatactt ctccatcttt ttctaagaac gtaaaattat
     3781 catgtttagt tcttattctc actttagcaa tatgattttt tccttttaac acaacttcta
     3841 taaatacttt ctctcttgta tcagcaggtg ttattgatag tggagtatta tccatataat
     3901 cctcagctat tttaacttct tcttttgtta gagtttctaa cacacttaat ccattttcag
     3961 aatggccacc tactatacct agagctgctg caatttttaa tcctaatctt tcagtacctg
     4021 gtatacctac gcacatgcca tttttgtaga tactaggact aactaacata cgattttcaa
     4081 ctatttcttc tcctaataat tcctttgcct ttgcacatgc tagagctaag gctactggtt
     4141 cagtacaccc tactgctggt ttaacttctg cctttaacat ttccactaat tcttttctta
     4201 tatctctcat tatatataaa cctcccaatt aagtttagat ttagtactca aataattaat
     4261 taaataattt cttatctata tttatagcaa aatacatgcc atatttttta tatcttttta
     4321 tatttttagt cttatattct ataaccattg gtatgactgc ttctatttat gtattttttt
     4381 tatttacata gagtatttat attctttaaa atggagaaac atatgacaat ttcgcaaaaa
     4441 gacaaaaaaa agtctcagaa tgagattcta tatcattttg agacggctca aatgtgttat
     4501 tttttaactt ataaaaacca ttttttaatt cataaaaaaa gattatgcaa aacacttata
     4561 gtataagttt aaagctttct ttaaagttat gaatttagaa aaatttaaat tttatatagc
     4621 tttttattat attactatga aattatattg tatttttcaa tttttctata taatgtagct
     4681 cttgatattc caagtgcttt agctgttaat tctttatctt ttttataatc tttgtactta
     4741 tttaaagctt ttattatctc aatcttctct agttcatcaa gtggaattat cctatcgctt
     4801 ttaacttcta actcttctac agaggtattc ttaatttttt gaggaattac attcattgtg
     4861 attattgaag aagaagacat attcacacta tattcaataa tattctgcaa ctctcttata
     4921 ttccctggcc acttatattc catcatagca tcaacaaccc tgctatctat gtcaataaca
     4981 tttttatcta atttttttga atactcttta accatatgct tgattataag tgggatatct
     5041 tcttttctct ctcttaaact tggtaatttt ataggtataa catttaatct ataatataaa
     5101 tcttctctaa aagtgccatt ttgaaccatt ttttctaaat ctttatttgt agcagcaata
     5161 attcttacat ctattgagac atttgattta cttccaattt tattaagttc cttttcttgt
     5221 aatactctca ataactttga ctgtaaatgc aaactcatat ctcctatttc atctaaaaat
     5281 atacttccct tatgagctac ttcaaacatt ccaagctttc cacctttttt agctccagta
     5341 aaagctccct cttcatatcc aaataattca ctctctaata aattgtctgg tatagcagca
     5401 cagtttacag ctataaaagg attatctact ctattgctat ggttgtgtat ggctcttgca
     5461 aatagctcct tacccgttcc actttcccca gtaataagta ctgttgatgt tgatttagaa
     5521 gctattaggg cttccttttt agaatttctt atagctacac tttctccaat tatgttttcc
     5581 aattttattc tataatcttt attcatctta ttgtagtttt ttatagcatc tcttttatct
     5641 ataaaatcaa taacatatcc ctttaactca ttttttaata ctattttatt gatgttgtat
     5701 attcccttcg aatcatatcc gtatctttta taaaaaaaac tacacgattt gtctttctta
     5761 aagttattta tagaaggttt ctttataaaa tctaattcga caaatatgtt tttgccagtt
     5821 atactatcct ttatattgaa aagctcttta aattttgaat tatatttatc aatttttcca
     5881 tccacatcaa tagacacaac agctttgtcc atattgttta atagtgtctc taattttttc
     5941 ttttctaatt caagctcata agttttaatt tgtgctttta atttattaga tattaagtct
     6001 gccattttgc ctaaaaagtt tataagacca cttttattat tttctataat ttgtgcttgt
     6061 tcttttgtaa aggctataag gcctataacg ccatgagaat cgccatcaca tataatagga
     6121 caacacacct ctgcaaactc tacacaagcc ccacttttat agcattccct acatacatca
     6181 ctactctttg ggtcatcaat tagtattgtg atctgttctg taagtgattt tttaaaagcc
     6241 gtatatttat ctactttctc acctattttg tctatatata ctcctgtacc tgcaatccta
     6301 aataactcct tatttactat agttacatct atattgagta ctgatactat agcttcagct
     6361 atattttgaa tatcagattt tatatattct aaacctatca taaccatcat tccttatttt
     6421 tctttaacat aagtatttgt cttatttctt taagaatcaa gtatatctta tttaaagtaa
     6481 attggattga catcaggatt acaaagagca taacaattat tattaactca taatttattt
     6541 ccatctaaac atctccttat aacaaaagac taggcttaac ctagtctttt atattatatc
     6601 catatttata ttttaactca tttatcttct ttttcccaca taaatatatt ttaatttatt
     6661 tgactcttta atcataccat caaagtttaa tcttgaaaat atattaaata cttcatgtgg
     6721 atagtgaatc tttgcaacag aaccacaaaa tttatgttta agataattta acttaacttt
     6781 taaattcttt aaatttaaat cctctaatat aacctctcca ttgcccttta acactctttg
     6841 tatttcagat gaagcttttt cacaatctgt aataacatta aattggtcaa ctattattat
     6901 cttgttgaac tgattatcat caaatggtag aaactctaac ttacctttaa ctacattgca
     6961 attttcatta tacactagag aataacagac atcttcataa ttactattct ccagtatagt
     7021 tacagaagtt ccaagacctc tcaatctctt gcctaggttt cctatatgac caacaagcaa
     7081 tattttatca cctacattat actcaattgt atccaaaaaa cattctaatt catcagatgt
     7141 atatttagct ttaactggct gtttatttat ctgacataat atgcttttca caatgcttca
     7201 cctcttcatt tcctcaatta taaattatgt acagattaca ttataataaa tggcgagcaa
     7261 ctattcaaga gatatttcat tagtattttt ttaaacaatc tttatacatt ttaatatgat
     7321 attataccaa atttattagg attttattca taaaaatcag tctatttttt atgtttattc
     7381 attgttattt tcatccaatt tttttatttt cgatatggat acttcatatg ccattttttt
     7441 gaccacatta ttttcatcta tttttttctc atattctcta ctttgaaccc taccccaaag
     7501 ctgtattcta tcaccaacct ttagattctt tgcaaattta gcatttctac cccaaactat
     7561 tgatggtata taatcagatt tattataagg tctgtttata gcaactaata aatcggttat
     7621 ttcccttcct aaaggtgtct tcctatacac aggttcctta catacatatc catctaaaaa
     7681 tatactatta gggtctttat tttcctcgtc tatgcttttt atttctctga caaacactgt
     7741 aagcactaat ctatttttat tatttaatgt tttgttatat gatcttaatt ggcctacgac
     7801 attgactaaa ttttgtttat caaggtcaat atcttggaga agtctttctg atacagtcat
     7861 tggaagtata tcaaaagaat cgcttagtct gtttattttt attttcatat tataaaattt
     7921 ttctccaaat atctcatggc taaattctag cttattgtct aactcccctc ttaagtttac
     7981 tacgttattc tcatctttaa cagctatcat attttatccc tccacaattg ttaattctta
     8041 tatttctggt atcagcttat ataataaata tattta
//
LOCUS       DACTGW010000171         8000 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.171, whole genome shotgun sequence.
ACCESSION   DACTGW010000171 DACTGW010000000
VERSION     DACTGW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.171"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            151..915
                     /locus_tag="I9573_002829"
     CDS             151..915
                     /locus_tag="I9573_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4797438.1"
                     /translation="MQIIDININNIDSRHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     AFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKI"
     gene            1605..2639
                     /locus_tag="I9573_002830"
     CDS             1605..2639
                     /locus_tag="I9573_002830"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAT4797439.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKNI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEVL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2681..3898
                     /gene="argJ"
                     /locus_tag="I9573_002831"
     CDS             2681..3898
                     /gene="argJ"
                     /locus_tag="I9573_002831"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HAT4797440.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKENDVLVASTGVIGKPLNINLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3913..4773
                     /gene="argB"
                     /locus_tag="I9573_002832"
     CDS             3913..4773
                     /gene="argB"
                     /locus_tag="I9573_002832"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAT4797441.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGMDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4803..5990
                     /locus_tag="I9573_002833"
     CDS             4803..5990
                     /locus_tag="I9573_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HAT4797442.1"
                     /translation="MNNNNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6099..7121
                     /gene="argF"
                     /locus_tag="I9573_002834"
     CDS             6099..7121
                     /gene="argF"
                     /locus_tag="I9573_002834"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAT4797443.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAYVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQKIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7248..7865
                     /locus_tag="I9573_002835"
     CDS             7248..7865
                     /locus_tag="I9573_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HAT4797444.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     TVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
BASE COUNT         3261 a          760 c         1486 g         2493 t
ORIGIN      
        1 tatatgcaat ttgataattc aaataatcta aaaataatat tatgtaaaat aaaaatatat
       61 atgacatgat gttgttgtat tcaaaatatt aagataaaaa tatatagaat atatttaaaa
      121 attattatgt aattagaaaa ggagataagt atgcagatta tagatataaa tataaataat
      181 attgacagta gacatatatg ctgttcaatt tctgaaaaaa aaggagaaaa ttgtgttttt
      241 tctaaaaaag cttggatgaa aaataatttt gataaaggtc tagcctttaa acgatttgat
      301 gtaagaggta aagcttttat agaatatatt ccagcagaaa atgcatggtg tcctattact
      361 gcggacaatt atatatttat taactgtttt tggatatctg gaaaatttaa gggtcaagga
      421 tattctaatc tattgctaca agagtgtgtt gatgatgcaa aatcaaaagg taaatatgga
      481 ttagttgtgc tttcgtctag taaaaaaatg ccttttttat ctgatgctaa atatctaaaa
      541 tataagggct tttgtttagc agatacatca gaaccaaatt atgagctatt atattttccc
      601 tttactgata atgctcctat tccatgtttt aagaattgtg taaaaaaaag aaaattaaat
      661 gagaacggta tagttttata ttacagtaat cagtgtccac atacagaaaa atatgctcct
      721 attttgaaaa aaattgcaaa ttctagaggt attgatgtaa atcttataaa attcgaaaca
      781 gcaaaagaag cacaaaatgc tcctgcacca tttactacat acagtctttt ttttaatggt
      841 gaatttgtaa ccaacgagat actttctgag aaaaagattg agaaattttt agataccaac
      901 attaaaaaaa tttaactaaa aatagatgta aatattgtat tttgagatta cattatttta
      961 taatccaata tattttggaa atttcttaag ttgatgaatg ataatataaa gattataact
     1021 caatttagat acaaaatgct ggaaattaac catatagaaa atatttccaa aaatttaaaa
     1081 attatattga caaagttaaa aaaataaact atcatataaa acaataagaa aataaattga
     1141 aatcctgtga aaaagagagt agcataaagc tagttgatag agagctggga atggtggaag
     1201 cctagtaacg aattttattg tgaatagagc tttggaggag attaccagaa gtaatagtat
     1261 ggtaattggt gaatctcgcc ttaagagatt agagtgggta agattatctt atcaattaga
     1321 gtggtaacgc gggtgtatat tcgtctcttg gtctttgacc aagaggcttt tttaatttta
     1381 aatgtacatt ttgtagatac tttttaattt gagttttaat gattattgta attttaccaa
     1441 tgagagtggt accgcggaaa atcgcctctt agtatttata atgctaaggg gttttttgtt
     1501 ttaattagaa tattttaatc tacatattat caaatgatta tcatatagta aagtggaaag
     1561 atagaatttt aatactattt gaatataaaa atggagggaa caaaatgaat aaaataaaag
     1621 ttggtattat tggcgcaact ggatatgtcg gggcagagtt gattagatta ttaatgaatc
     1681 atgataaagt cgaagtgaca gcaataggtt caaattcgta tgttggaaaa aatatagttg
     1741 atatatatcc aagtatagga tataaaaata acatgatatg tatagaaaat gaaaaagtta
     1801 tagatatgtg tgatgttgtg tttacagccc ttccacatgg agttagtgaa aagtttgtaa
     1861 taaaagcaat taaatcgaaa aagaaagtta tagatttagg tgctgacttt agaataaaag
     1921 atgaagaagt atatagtaaa tggtatggag tatcttttat agataaaatc ttacataaaa
     1981 aagctgtata tggattaagt gaaatataca aagaagatat aaaagatgca gatataattg
     2041 caaatcctgg atgttatcca actagtattt ctttaccatt gatgccatta ctaagttcta
     2101 aattgataaa aaataataat ataataattg attctaaatc aggtcttaca ggtgctggta
     2161 gagaattatc gattagtagt cactttacgg aggttaatga aaatatcact gcatataaaa
     2221 tagggaaaca cagacatact ccagaaatag aacaaaattt aagtgaatcc tgcaaagaaa
     2281 aagtaagtgt agtttttaca ccaaacttaa taccagtaaa taggggaatc ttatcaacta
     2341 tatattgtac aaaagaagat aatatttcta taaatgatat acatagaaaa ttaactgatt
     2401 attatgaatt taaagaattt atagaagttc tcccattaga taaggtagct tctttaaaga
     2461 atgttaggtt ctcaaataaa tgtgtcatat cattacatga aaatggagat acattgataa
     2521 tctgttcagc tatagataat atgattaagg gagcagcagg tcaagctata caaaacatga
     2581 atataatatt tggtattgaa gaaaatacag gtttaaaaaa tattgcacca tcattttagt
     2641 aatgaaaaaa tactaaactc atataattag gaggataaat atggagatta taaagggtgg
     2701 agttacagtt tcagaaggat tttttgcttc agggattcac tgtggcttga gaaaaaataa
     2761 agaaaaaaga gatttagctt tggtttattc agatgtttta tgtgatgcag cagcagtagt
     2821 aactcaaaat aaagtaaagg gcaatcctgt ttatgttaca caggaacaca ttaaaaatgg
     2881 aaaagcacaa gctatcatag taaatagtgc taatgctaat acctttaatg gaaaagaagg
     2941 acttataaat gcttataaaa tggctaaatt cactagtgat aaattaaata ttaaagaaaa
     3001 tgatgttttg gttgcatcta caggtgtcat aggaaagcca ttaaatataa atttaataga
     3061 agaaaatatg gatgaattag tgaataatct aagtaaacaa ggtcatattg gagcaagaga
     3121 agctattatg acaactgata ttataaaaaa agaaatagca gtagcaataa tgtatggaga
     3181 taaaaaaata actattggag gtatggcaaa aggttctggc atgatacatc ctaatatggc
     3241 aacaacgtta ggatttataa ctacagatgc aaatattgat ggagttttgc taaaagaagc
     3301 tttgaaaatt gctgttgata aaagttttaa tagagttagt gtagatggag atacaagtac
     3361 taatgatatg gtgatgattc tagcaaatgg aaaagcaaaa aatgatagaa taaacaaaaa
     3421 agatgaacat tatcaagtat ttttgagtgc acttacatat gtctgtatag aacttgctaa
     3481 acttgttgca aaagatggag aaggatgtac aaaactaatt gaatgttata taaatggagc
     3541 attaagtgaa gaacatgcag taaaattagc aaaaacagtt atatcttctt cattagtaaa
     3601 gacagctgta tttggagctg acgcaaattg gggtagaata ctgtgtgctt taggttatgc
     3661 aggtgaagaa atagatatgg aaaaagttga tattattttt gagagtatga aaggatatat
     3721 tgaagtatgt aaaaatggaa atggattaga ttttaatgag gaaaaagcaa aaaaaatcct
     3781 tgaagatgac gaaataagta tattagtaga tttaaatatg ggcaatgcca gaggaaatgc
     3841 atggggatgt gatttaagct atgattatgt aagaataaat ggaagttata gaaattagga
     3901 ggatatactt taatgattaa tatagaaaaa gcaaatacac ttatagaagc tttaccatat
     3961 atagaaaaac atcagggaaa gactattgta gttaagtatg gtggaagtgc aatgaaaaaa
     4021 gatggtctaa aagaatctgt tatggaagac cttgtattaa tgagttatgt tggaatcaat
     4081 atagttttag tacatggtgg aggggcagaa atcaataaga tgctagcaaa agtggatata
     4141 gaaagtaaat ttgtaaatgg tcttagatat acagatgaag agaccatgga aattgtaaaa
     4201 atggtacttg ctggaaaggt taataaagac ctagtaaata aaatacatac aaaaggtgga
     4261 aaagcagtag ggctttgtgg aatggataat aatatgattt tatgtgaccc atataaaaat
     4321 tatgaacttg gatttgtagg agaaatcaaa aaagtaaatg ttgagcttat agaatcttgt
     4381 ctaaagtcag gttatatttc agtaattgca actataggtg ttggagatga tggagagact
     4441 tataatataa atggtgacac agcagcttct gctatagcta aagagttaaa tgcagataaa
     4501 ttgatacttc ttacagatgt ccctggtttg ttaagagagc cagatgaaga aaaatcatta
     4561 ataacagaag ttatattaga ggatgttgat aaattatttg aagaaggtat aattactgga
     4621 ggtatgatac ctaaaataga gggatgcgta gatgccttaa ataacggagt aaatagggta
     4681 catattttgg atggaagagt tccacattct ataataacag agctatttac tgatagtgga
     4741 ataggtacat taataagaaa agaaaatgaa taaaattata agtttaagag gggagaataa
     4801 gaatgaataa caacaataca atttcaaaat ggaatgaata ttttatagat acatataacc
     4861 aacccaattt tgtcatagat tatggtgaag gaagttgttt ttttgatact aatggcaata
     4921 aatacataga ttttacaagt ggatatggag tatcttctct tgggtatagt aatagtaatt
     4981 taaagaatgc tctaaaagag caagtagata aattattaca tacttcaaat ttatatttta
     5041 atgagccagt gttgtattca ggagagaaaa taattaattc aagtggaatg gctaaagtat
     5101 atttttgtaa ttctggaaca gaagcaaatg aaacagcatt caaaatagca agaaagtata
     5161 gtagtgacaa atatggtaat ggtagaggaa ctatcataag tttaaaagac tcttttcatg
     5221 gtagaacaat gatgtcttta atggcaactg gtatggacaa ataccataag tatttttatc
     5281 ctcttcccga aggttttaaa tatatagaaa gaaataatat tgaagattta aaaaataatc
     5341 ttgattccac agtttgtgca ataattcttg aagcgattca aggcgaaggt ggagttaatg
     5401 ttcttgaaaa agattatgtt ttggaaatag taaaaatatg tcaagaaaag gatattgtag
     5461 tgatttttga tgaagtccaa tgcggtatag gaagaactgg taaacttttt ggctatgaat
     5521 attttgatgt aaaaccagat atagttaccg tagcaaaagg gttgggagca ggaataccag
     5581 taggaggagt attggtaaat aaaaaacttt caaaagtact aggaaaagga gaccaaggta
     5641 caacttttgg aggtaattta ttagctatgg tggcagcttc agttgtatta gatgagatat
     5701 ctaaagatgg tttttataat gaagtattag aaaaaggaaa ttatataaga aaatctattg
     5761 aaagttttaa taataaagtt gtattaaaga caaaaggtat tggtctaatg ataggaattg
     5821 aaactaatat tgaatctagt attatagaag agaaagctag aaaaaaagga ttgcttattt
     5881 tgactgctgg aaaaaatgta ttgagatttt taccaccatt aacaatcagt tataaggaaa
     5941 tagatgaggc tcttgaaatc ctaaaagata tattactaga gattaactaa atattcaaaa
     6001 aatagttaat taaagataaa aattagaagt aaataaaaaa gtaaagtgct aacaagttta
     6061 attgaattta agaagtttat aagaaggggc gaattaatat gaataatata agtttaaaag
     6121 gtaagagttt tttgacatta aaggatttta caaaagaaga gattagatat ttattaactt
     6181 tatcaagtta tttaaaaact aagaaaaaag ttggcataaa aggcgatttg ctacaaggaa
     6241 aaaatatagc tttattattt gagaaaacat caacaagaac tagatgttca tttgaagtag
     6301 cagctcatga tgaaggagca tatgtaactt atctaggtcc aaatgattgt catatgggta
     6361 aaaaagagtc tgtatcagat acagcaaaag ttctaggaag attctatgat ggaatagaat
     6421 ttagaggctt taaacaagaa acagttgaaa gtctagcaaa atattctggt gtacctgttt
     6481 ggaatggact tacagatgaa tatcatccaa cacaaatact ggcagatttt ttaacagtaa
     6541 ttgaaaatgt agataaagat ttgaataaag ttaaatttgt atatgttggt gatgctagaa
     6601 ataatatggg aaactcactg atgataggat gtgctaaaat gggaatacat ttcgtagcac
     6661 tagcacctaa ggaactttgg ccaaatgaag aattggttaa ggaaatgaag gaaatagcca
     6721 agaaatccaa aggagatata atgttgactg aaaatattga tgatgttaaa ggtgcagatg
     6781 ttatatatac agatgtatgg gtttctatgg gagaagagga gcaatatgaa gcaagaataa
     6841 atcaacttaa agattatcaa gtaaatatgg atatgataaa gaaaactgaa aatgaaaatg
     6901 ttatattttt acactgtctt ccagcatttc atgatttaga aactaaggtt gctcagaaga
     6961 tttatgaaaa gttcggatta tcagaattag aggttacaaa cgaggtattt aattcaaaat
     7021 attcaaaggt atttgaagaa gctgaaaata gaatgcatac aataaaagct gttatggttt
     7081 ctactatgac tgatgtaaat atgaacaata atttaatata gatatgtttt aatatagact
     7141 ttactttaat gaaaatttat aaaatatttt aaatatatta ttcaaaaact ctcattatat
     7201 gttaaaataa cttatgtatc aacaaattaa gagggaggat ttgagatatg aaaaaaatca
     7261 tattagcttt aacaactttt ttattagcaa tatctttaac agcatgttca agtgcaaaac
     7321 cagaagatac aattgatagt ttttttaaca gtgcgaaaaa gtttgacttt gaaggcatga
     7381 ataaggtaat ggaaaataat gacgaaaaat ataaggatat attaaaggaa ttagatacaa
     7441 aagacccaaa tgcccaatat gttttagatt atctaaaaca gaatgcatct aaaatcacgt
     7501 atacaataaa agacagcgag gttaaaggtg ataaggctac aatcaaagta gaatgtaaat
     7561 ttatagattc aacaccatta cttcaagaaa tagtagctga ggcatttaca aagatgattg
     7621 gtatgagttt ttcaggacaa gacttaactg atgagaaaac aactgaaatg ttagtatcaa
     7681 ttatgaaaga aaaacaaaaa agcgttaaag agacttatgt tacaaaaact gtagagtttg
     7741 aatgttctaa aaaggataat aaatggataa taagttcagt aaatgatgca gtagctgatg
     7801 tattgttatc taatcttgtt acagcaggac aagaattttc taactctatg aatacacttg
     7861 aataatattg tttaggtgta gtaaagtaat aaatatatga ttatttttat aaataaacat
     7921 ataaatattt atgaaggata ccatttttgg tatccttttt attaatataa tgaaatacat
     7981 ggttaatttc tatattgtca
//
LOCUS       DACTGW010000172         7974 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.172, whole genome shotgun sequence.
ACCESSION   DACTGW010000172 DACTGW010000000
VERSION     DACTGW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.172"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            124..1983
                     /locus_tag="I9573_002836"
     CDS             124..1983
                     /locus_tag="I9573_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT4797445.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVC
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICINKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEEILDKEFVYNS"
     gene            complement(2275..2874)
                     /locus_tag="I9573_002837"
     CDS             complement(2275..2874)
                     /locus_tag="I9573_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tellurium resistance"
                     /protein_id="HAT4797446.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2977..3774)
                     /locus_tag="I9573_002838"
     CDS             complement(2977..3774)
                     /locus_tag="I9573_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAT4797447.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3887..3992)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4084..5070)
                     /locus_tag="I9573_002839"
     CDS             complement(4084..5070)
                     /locus_tag="I9573_002839"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAT4797448.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLNEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5554..7284
                     /locus_tag="I9573_002840"
     CDS             5554..7284
                     /locus_tag="I9573_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HAT4797449.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7394..7474
                     /locus_tag="I9573_002841"
     tRNA            7394..7474
                     /locus_tag="I9573_002841"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7428..7430,aa:Leu,seq:tag)
     gene            7706..7879
                     /locus_tag="I9573_002842"
                     /pseudo
     CDS             7706..7879
                     /locus_tag="I9573_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
BASE COUNT         2855 a         1004 c         1232 g         2883 t
ORIGIN      
        1 aacaaggttt atatattcta atagtataga aaattttatt gtaaacgcat gtatattatt
       61 agaaaaaata gtttgaaggt attcaataaa cataaaaaac aatatagggg ataaaggtgt
      121 tttatgaaga gatttttgag gagaattatc ttagttttat ttattttgtt gatttttata
      181 agttttattt ctattaaatt aattcataat gttggagatt atggaaaatt gattaattat
      241 gttgggattg tttgtggagc tagccaacgt ctaactaaac tagagatgaa tcatcaacca
      301 aatgatgaat taatagaata tattgatgaa atcctacagg agttgattac agggcatgga
      361 gattatggtt tagtaatcac tgattgtaat gaatacaatg aggacttact tttattagaa
      421 aaaaagtggg aagatttaaa tattgaaatt aaaaaagtaa gaatgaaaga acagaataac
      481 cagttattaa gtataagtga agagtttttt tctcttgcaa atgatacagt gtttaaaata
      541 gaaaactttt ctaaagaaaa aagtaattac ttaatgactt taataataat tatatctatt
      601 ataggtatat tagcatgtat catattgatt ttacaatata gtaaaaaaat ggttagactg
      661 gaaaaattaa atgtagattt aaaaaatatt gcatataaag atgaactaac aggagtaaat
      721 actatagaaa aatttaaatt ggatgcaaat caaaatattt gtataaataa ggataagaaa
      781 tttgctgtat tctatattga ttttgaaaac tttaaataca ttaatgatat atttggatat
      841 gattatggtg acatgatact gaaaagatat gctaatctta tgatgaatga catagggaaa
      901 tatgaaatat ttgcaagaga aattgcagat agatttgttg cgttaagatg ctatgtagat
      961 aaggaagatt tagtagtaag acaacgagat gtagacaatg aactaattaa tataacaaat
     1021 gaaattaaaa ataaacatag tattactgtt gtcagtggaa tttgttgcat tgaagatgta
     1081 aatgaaaaat taagtataga tggtttaata aatagagcaa attttgcaca aaaaactgtt
     1141 aagaataaac caggtactaa ttatgctttt tataatgaca gtattcgtaa aaaaatgatt
     1201 gaagaaaata ctatcaagag tagaatacat gaagctattg aaaaaagaga atttatagtt
     1261 tatttacagc ctaaagttaa tttacatagt caaaaaataa attgtgcaga agctttagtt
     1321 cgttggctta ctccggataa aggtattata tctccagcaa tatttattcc agtacttgag
     1381 aagaattttt ttattgcctt agtagataag tatgtttttg aggaggtttg taaatggatt
     1441 agaaagaggt tagatgaaaa taaacctttt gttcagatat cagttaatgt ttcaagaata
     1501 caattttata acactaaatt tgtagaaact tattccaata tacaaaataa atacaggata
     1561 cctaaaaata ctatagaaat tgaatttaca gagagtgttg cttttgagaa tcaaaaacat
     1621 cttttggaaa ttatacatga tttgcatgaa aatggattta cttgttcttt agatgatttt
     1681 ggaaaaggtt attcgtcttt aagtgtttta aaagatttac catttgatgt tttaaagctt
     1741 gactctatgt ttttcaaggc tagtttagat aaagataaag agaaaatagt aataaaaaat
     1801 attgtacata tgttaaaaga gcttaatatc acaactgttg ctgagggcat tgaatgcaag
     1861 gaacaagttg aatttcttag ggatattggg tgtgatttag ttcagggatt tgtattttat
     1921 aaaccaatgc caatattaga atttgaagaa atattggata aagagtttgt ttataatagc
     1981 taaaatgtac tttataaaat atgaactaat taagtaaaat tattttgtac tgaataaatt
     2041 tttactttat ttacagaata gtgattattt acagaaaagg gcgaaaagtg tattccttta
     2101 agtatgggat ggatagcaca aataggggag aaaaaatatt ggccatataa gaatgcataa
     2161 taatataaat atttgaaagg tcagcattat ataaaaatta aaaagaaaat aaataatttt
     2221 tctataaaaa agagactatg agaaaagtta ctcatagtct cctaaataat gtatttattt
     2281 tcttcctgca acccatttta agccccaatg aaattcagta tccatttctc tatgaccttt
     2341 gaagtattta actacttttt caacactgaa tgtctcatca tttacatttt ctatcaatgc
     2401 tattgaacac atattatagc catttttatg ttcatcaagt ttgacaacta agtctgaatc
     2461 ttgtttttgt ttgattgtta caattccatc agcttcagac cagttagcta caccttcgta
     2521 tataaaagca taaataagta ttctttttat atcttttatt ttattaccat ttattcttaa
     2581 attttctcct tgtgtattac ttcctgttct gtcatctcca tctaattgag caaaaggtgg
     2641 attgtctaaa ctaccaaaag catttcctaa tgcttgaaca gcacctttta caccattttt
     2701 catctcaaat aggcaaccca aatctaaatc tatattactg cttcttaaag agccaaaaaa
     2761 accctttttt tgtacttttt gattccaatt taaattaact aagatttcac ctaaatcgct
     2821 attttccttt tttgtaaggt ttattttttg tccctttttt aattctatag ccatagtaat
     2881 cttcctccac aaacatagat ttatttaaat ggtagcactt aataagtcaa tattcaacaa
     2941 gattagaata ataatattaa aactatacta aatatactaa aaagtaggaa tcacaatacc
     3001 tttgtatttt tcttcaatta agcttcttgc tttatctgat gtaagaacat cttgtagaac
     3061 tttaatcttt tcactgtctt tatcttcagt tctaaatgct aaaagatttg catactcgtc
     3121 atttatagga gctgtataaa ttccgttttt cttggcatca agaccagcag gtacagcata
     3181 agtagtatct ataaatgcag cagtaacatc tggaagagaa gatggaattt gagcaatatc
     3241 taactctttg aattgtattc cctttggatt ttctattatg tccttaggac taactaatcc
     3301 atccccttta ggtaacgtta aaagtccaat actttcaagg tatctaaggt tcttagagca
     3361 agaagatggg ttattgctta ttgcaattgt atctcctttt ttaaattcat caacagattt
     3421 tatcttataa gagtaaagta ttgctgggca tacatatagt tttttaccag ccattatttt
     3481 gtaacctttg gccttaacag cttcttttag ataaggttca tgttgaaata gatttgcatc
     3541 aatttcacca ttatttaaag cttcgtttgg aagtatgtaa tcatcaaagt ttttaacttc
     3601 taaagtatat cctttttctt taataagtgg ttttaattct tctaataact ctcctcctgg
     3661 aactaaagta gcaccaacaa caattttttt atcatcttta gctttagaac atccaaccac
     3721 agctaaagtt agtactagac ataataacaa acataataat tttttgaatt tcatataaat
     3781 tccctcctga atataattga ttagttaaaa ataaaatacc attctttatc acactgcaaa
     3841 ataaaaataa gcctgagttt aaactcaggc tttaaaagct aatttagact catctttcag
     3901 tataatactg caggatttag caccaatgta tataaaaata tactggttgc tgggtttcat
     3961 agggccagtc ccttcaccac tctggataag aagtatcaat ttttaattat gttataaata
     4021 ttatcatgat tttttacgtc tgtaaacata attttataaa tattttaaaa tctttaatgt
     4081 attttattta acacctaaaa ttccagaaac tagaatatca gtatcagcac ctaagaaata
     4141 gtcatttatt tcaatagaat ccagagtatt tctcaaagca ttttcttcat gccttatacc
     4201 aataagttta ttttctacaa atgcaacatc acattttcca aaaaaatctc caaaaaattt
     4261 tatactagtt attatgcctt tatcaacatt taaattaaac tcaacatttc caccagaata
     4321 ttttaattca tttgaaagag tatattttgg tgaattacca aaattccaat cccaagtttc
     4381 gtattttgat ttagtaagct tttctatttg ttttatatca tgctcattca aacaataatt
     4441 agtattatca gtatttgttt tagaaagata atccattata gcatctttaa attctaatat
     4501 gtccattttt gaatgtaaat gttcacttat attggttaca cgagatttta cagattttat
     4561 tccttttcct tcaaatttta taggtttaac ctttaatgct tttgagaggt catttatttc
     4621 agatgaaaat aaaagagtac catgatgcat tactttgttt ttgtagttgt attgagcatt
     4681 tccagaaaat ttcttgccat ctataagaag gtcatttctt ccagaaaatt cggcattgac
     4741 atctagtgtt ttaagtaaat ctattatggg ctgagtgaat cttctgaaat cactaaaatt
     4801 attattgtta caagctataa atgtaaaatt tatatttccc aaatcatgaa atacagctcc
     4861 accaccagac tgtctcctaa cgacaggtat agaatattct tttatatatt ctaaatttat
     4921 ttcagaaagt gtgttttggt tttttccaac aattatagaa ggttcattcc tccataaaat
     4981 aaataagtct tcatcaaaat tttttagaag atattcttcc attgctaaat taaaataagg
     5041 gtttgttttt tcattataaa ttaataacat taaagtagtt cctccataga attgataaaa
     5101 tttttattat gaatacaatt aattttataa ttcgacaata atataaaaac tcctttaaat
     5161 tagaaatttt aaaaactacc ttgcaaagtt taaataaata atatatactc aaaatatgtt
     5221 taataaaagt gaatagaagg gtagaggcgc aataatctat cagtactaat aaggagttaa
     5281 gcacagagag ttattaggaa aggagatatt gccgaagttt tataatcgat gctttaagat
     5341 tatattgctg ggggtacata gaaaatatgt acaactgtca caggtttatt tgtggtgagc
     5401 tatcatttaa ggaattatat tatatttatt tttactattg ttgatatatt gtaatgttta
     5461 agccttgagt gtttaccact caaggctttt ttagttttat tgtaacataa taccctgtac
     5521 agttcacaaa attaaaagga gagggagatt tatatgagga acaaaaaaat taatattatt
     5581 tttctgacga caatcatgtt cataatgtca acagtaatgg tttttgcaga agaagatata
     5641 gacactattg cactagctaa tgcagaaaaa tttgggattt taactttaat accaccatta
     5701 gttgctataa tacttgcatt tataactaaa aacgtaataa tttctttact tataggtatt
     5761 ctttctggta gttttataat taaagctagt ggtattaatg tttttgcgac atttatacag
     5821 gcatttttag atttagtaga tagagcttta gtatctttag cagacccttg gaatgcaggg
     5881 ataatactac aggttttggc aattggagga gtaataaatc ttgttgctaa aatgggagga
     5941 gctaaagcta tagctgaagc acttgctaaa agagcaaagt cagcaaaagg gacacagctt
     6001 ataacatggt ttttggggct tttagtattc tttgatgatt atgccaattc actaatagta
     6061 ggtcctatga tgagacctgt tgcagataaa atgaaaatat caagagaaaa gctagcattt
     6121 ataatagatg ccacagcagc accagttgct ggtttagcta taatatctac atggataggt
     6181 cttgaggttg gtttaataca tgatgctttt gaaagtataa gtatagatgt agatgcattt
     6241 ggtatatttt taaatacaat accatttaga ttttacaata tattaatatt agctttcatt
     6301 gtaatatcag cattattatt aaaagaattt ggtccaatga gaaaagctga gataaaatct
     6361 agaagtagaa aaattagtat agaccttgat gaaggcgtag aagagttgga tgatttagct
     6421 ccaaaaaatg gagtaaaatt gagtgtatgg aatgctataa tcccaatagg aacattgata
     6481 atagttgcat tagcatcatt ttattatagt ggatatacat ctataatggg aggagacgat
     6541 aaggctctga tacaactatt tacaaattct ccatattctt ttgaagcaat aaaagaagca
     6601 tttagtgcat ctgatgcatc tagagcatta tttcagtctg cacttgttgc aagtttagtt
     6661 gctattataa tggctgtagt aaaaaagatt tttacaatat cagaagcaat tgatgtttgg
     6721 atagatggaa tgaaaagttt agtaatcact ggtgtaatat taatacttgc atggtcatta
     6781 agttcagtaa taaaagagtt aggtacagct aagtttttaa tacacctatt atcaggttca
     6841 ctaccaccat tcttattacc aagtttaata tttggtttag gagcaataat atcttttgct
     6901 actggaacag cttatggaac aatgggtata cttatgccac ttgcaattcc attagcatac
     6961 tcattaaatc ctgatatgtc ttatgtaata gtaagtacta gtgcagtttt aacaggtgca
     7021 atatttggag accattgttc tccaatttca gatacaacaa tactttcttc aatgggagct
     7081 ggctgtaatc atatagacca tgttaacact caaatgccat atgctatatt tacagcagta
     7141 atcactattg tatttgggta tataccagct ggacttggat tgccaatata tatagtatta
     7201 ccagtggcaa tagctgctat atttgtaggt attcaaataa ttggtaagaa agttgatgaa
     7261 gcggaaatag aacttgtaga ataaagatat aaataagttt caccttttat tatcactatt
     7321 ttgttagttt ttagataatt ttaaaaagtt attgaaaata atggcgaaat atagtaaaat
     7381 aaaagagtga taagcggatg tggcggaatt ggcagacgca ctagacttag gatctagcgc
     7441 cattggcgtg ggggttcgac tcccttcatc cgcactttac agtacttaat tgtactgaaa
     7501 aaaactgggg ttgctggctt tcggagtggg ctatcccgtt tttatttttt tgtatatata
     7561 atagattggc actttagtat aaggtgcttt ttttataggc aaaaacagta catttatttc
     7621 tgttttatta tcattcatct attagaatat actttattta agaatgtgtt aataagcagt
     7681 gttcacattc ttgataaaat gtggaataat atgcctacca ataagcattt ggtgtataag
     7741 taagagtatt tatatagttg attgtgaaat gagtacaaca attctgtaca gtataattgg
     7801 cattataatt gtagcattga atttaaagtt tgcacagtcg ctgattggaa aatttacaaa
     7861 gtggatgtca aacatataaa ttttagttta ttggacagtt caaggaatca gaaattggaa
     7921 ttttatagta ttaatatttt atagtattaa tataatgtat aaaattttat agca
//
LOCUS       DACTGW010000173         7965 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.173, whole genome shotgun sequence.
ACCESSION   DACTGW010000173 DACTGW010000000
VERSION     DACTGW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.173"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            289..1488
                     /gene="hadA"
                     /locus_tag="I9573_002843"
     CDS             289..1488
                     /gene="hadA"
                     /locus_tag="I9573_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HAT4797450.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            1527..2327
                     /gene="hadI"
                     /locus_tag="I9573_002844"
     CDS             1527..2327
                     /gene="hadI"
                     /locus_tag="I9573_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HAT4797451.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            2342..3568
                     /gene="hadB"
                     /locus_tag="I9573_002845"
     CDS             2342..3568
                     /gene="hadB"
                     /locus_tag="I9573_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HAT4797452.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            3568..4695
                     /gene="hadC"
                     /locus_tag="I9573_002846"
     CDS             3568..4695
                     /gene="hadC"
                     /locus_tag="I9573_002846"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HAT4797453.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKNETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            4743..5876
                     /locus_tag="I9573_002847"
     CDS             4743..5876
                     /locus_tag="I9573_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAT4797454.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            5909..6694
                     /locus_tag="I9573_002848"
     CDS             5909..6694
                     /locus_tag="I9573_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005367473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAT4797455.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            6713..7750
                     /locus_tag="I9573_002849"
     CDS             6713..7750
                     /locus_tag="I9573_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAT4797456.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
BASE COUNT         3175 a          966 c         1586 g         2238 t
ORIGIN      
        1 gcaatggata gtggcaagat gccacgatat gaaaaagatt attcaattgc atattgttaa
       61 aaaacattca aatcaagcga tattttgatt aaatataaag ttggcacgat aaatgcttaa
      121 caatataggt gtaagacaat aaaacaataa aaaataattt taataatttt taagttattt
      181 tagataagat taggaacttt gacatcagac agttaatatc agaaaaaagt attttatgag
      241 tgatttaatc gaaattttat aattgatact attaaggagg aaaacgttat gcttttagaa
      301 ggagttaaag tagtagaact ttcaagtttc atcgcagcac catgttgtgc aaaaatgtta
      361 ggtgactggg gtgcagaggt tattaagatt gaacctatag aaggtgatgg aataagagtt
      421 atgggtggaa catttaaatc tccagcatca gatgatgaaa accctatgtt tgaattagaa
      481 aatggaaata aaaagggtgt aagtattaat gtaaaatcaa aagaaggagt agaaatatta
      541 cataaattat tatcagaagc agacatattt gtaactaatg ttagagttca agcattagaa
      601 aaaatgggta tagcttatga ccaaataaaa gataagtatc caggattaat attctctcaa
      661 atattaggat atggtgaaaa aggaccttta aaagataaac caggatttga ctatactgca
      721 tacttcgcaa gaggaggagt tagccaatct gttatggaaa aaggaacatc tccagcaaat
      781 acagcagcag gatttggtga ccactatgca ggtctagcac tagcagcagg aagtttagca
      841 gcattacata aaaaagctca aactggtaaa ggtgagagag taacagtaag tcttttccat
      901 acagctatat atggaatggg aacaatgata acaacagcac aatacggaaa tgaaatgcct
      961 ttatcaagag aaaatccaaa cagcccatta atgactacat ataaatgtaa agatggaaga
     1021 tggattcaat tagctttaat acaatacaac aagtggttag gcaaattctg taaggttata
     1081 aatagagaat atatattaga agatgataga tataataaca tagattcaat ggttaatcat
     1141 gttgaagatt tagttaagat agttggagaa gctatgttag aaaaaacatt agacgagtgg
     1201 tcagctttat tagaagaagc agacttacca tttgaaaaaa ttcaaagctg tgaagattta
     1261 ttagatgacg aacaagcttg ggcaaatgac ttcttattta agaaaacata cgatagcgga
     1321 aatacaggtg tcttagttaa tactccagtt atgtttagaa atgaaggaat taaagaatat
     1381 acaccagcac caaaagtagg tcaacatact gtagaagtat taaaatcttt aggctacgat
     1441 gaagagaaaa taaataactt taaagatagt aaagttgtaa gatattaatt agtaattcta
     1501 gattttttaa aaggagaatg ctaaagatgt acacaatggg attagatata ggttcaactg
     1561 catcaaaggg agtaatctta aagaatgggg aagatattgt agcttctgaa acaatatcct
     1621 ctggtactgg gactactgga ccatcaagag ttttagaaaa attatatggc aagacaggtc
     1681 ttgcaagaga agatattaaa aaagttgtag ttacaggata tggaagaatg aactattcag
     1741 atgctgataa gcaaataagt gaattaagct gtcatgctag aggggtaaat ttcataattc
     1801 cagagacaag aaccattatt gacataggtg gtcaagatgc aaaggtatta aaattagata
     1861 ataatggaag actattaaac tttcttatga atgacaaatg tgctgcaggt acaggaagat
     1921 ttttagatgt aatggcaaaa ataatagagg ttgatgtatc tgaactcgga agtatatcta
     1981 tgaattctca aaatgaagta tcaataagca gtacatgtac agtatttgca gagtctgagg
     2041 ttatatcaca tttatctgaa aatgcaaaaa ttgaagatat agtggcaggt attcatactt
     2101 cagtagcaaa gagagtttct agcctagtaa aaagaatagg agtacaaaga aatgtagtta
     2161 tggttggtgg agttgctaga aatagtggta ttgtaagagc tatggcaaga gaaatcaaca
     2221 cagaaattat tgtacctgat atacctcaat taactggtgc tttaggagca gcgttatatg
     2281 cttttgatga agcaaaagaa tcacaaaaag aagtgaaaaa tatataaaaa gagggatgaa
     2341 aatgtctgaa aaaaaagaag ctagagtagt aattaatgat ttattagctg aacaatatgc
     2401 aaatgcattt aaagctaaag aagaaggaag acctgtaggt tggtcaacat cagtatttcc
     2461 tcaagagtta gcagaagtat ttgacttaaa cgtattatat ccagaaaacc aagcagctgg
     2521 agtagcagct aaaaaaggtt ctttagaatt atgtgaaata gctgaatcta aaggatattc
     2581 tattgaccta tgtgcatatg caagaacaaa ttttggtctt ttagaaaatg gtggatgtga
     2641 agctttggat atgccagctc cagatttcct actttgctgt aacaatatat gtaatcaagt
     2701 tataaaatgg tatgaaaata tttcaagaga attagatata cctttaataa tgattgatac
     2761 aactttcaat aatgaagacg aagttactca atcaagaata gattatatta aagctcaatt
     2821 tgaagaagct ataaaacaac tagaaattat atcaggaaag aaatttgacc ctaagaagtt
     2881 tgaagaagta atgaaaatat cagctgaaaa cggaagacta tggaagtatt ctatgagttt
     2941 accagcagat tcttctcctt ctccaatgaa tggatttgac ttatttactt acatggctgt
     3001 aatagtttgt gctagaggta aaaaagaaac tacagaagca tttaagttac ttatagaaga
     3061 attagaagac aacatgaaaa ctggtaaatc ttctttcaga ggggaagaaa aatacagaat
     3121 aatgatggaa ggtatacctt gttggccata tataggatac aagatgaaaa cattagctaa
     3181 atttggagtt aacatgacag gtagtgttta cccacatgct tgggcattac aatatgaagt
     3241 taatgattta gatggaatgg cagtagcata tagtactatg tttaacaatg taaacctaga
     3301 ccgtatgaca aaatatagag ttgattcttt agtagagggt aaatgtgatg gagcattcta
     3361 tcatatgaac agaagctgta aacttatgag tttaatacaa tatgaaatgc aaagaagagc
     3421 agctgaagaa actggattac catatgctgg atttgatggt gaccaagcag accctagagc
     3481 tttcactaat gctcaatttg aaacaagaat tcaaggttta gttgaagtaa tggaagaaag
     3541 aaaaaaactt aatagaggtg agatataatg gaagctattt tatctaaaat gaaagaagta
     3601 gttgaaaatc caaatgcggc tgtaaaaaaa tataaaaatg aaactggtaa aaaagctata
     3661 ggttgtttcc cagtttattg cccagaagaa attatacatg cagctggaat gcttccagtt
     3721 ggtatatggg gaggacaaac agaattagat ttagctaaac aatatttccc tgcatttgca
     3781 tgttcaataa tgcaatcatg tttagaatat ggattaaaag gtgcttatga tgaattatct
     3841 ggagttatta taccaggtat gtgtgataca ctaatttgtt taggacaaaa ctggaaatca
     3901 gcagtacctc atataaaata tatatcatta gtacacccac aaaatagaaa acttgaagct
     3961 ggtgtaaaat acttaatcag tgagtacaaa ggtgtaaaaa gagaacttga agaaatttgt
     4021 ggatatgaaa tagaagaagc aaaaattcat gaaagtatag aagtttacaa tgaacataga
     4081 aaaactatga gagactttgt tgaagtagct tataaacatt ctaatactat aaaaccatca
     4141 ataagaagct tagtaattaa aagtgggttc tttatgagaa aagaagaaca tactgagcta
     4201 gtgaaagatt taatagcaaa attaaatgct atgccagaag aagtctgttc tggaaaaaaa
     4261 gttttattaa caggtatatt agctgattct aaagatatat tagacatttt agaagacaac
     4321 aatatatcag ttgtagctga cgacttagca caagaaacaa gacaattcag aacagatgta
     4381 ccagcaggtg atgatgcgtt agagagatta gcaagacaat ggtcaaacat agaaggatgt
     4441 tcattagctt atgaccctaa gaaaaaacgt gggtcactta tagtagatga agttaaaaag
     4501 aaagatatag atggtgttat cttctgtatg atgaaattct gtgacccaga agaatacgat
     4561 tatcctttag ttagaaaaga tatagaagat agtggaatac ctactttata tgttgaaatc
     4621 gaccaacaaa ctcagaataa tgaacaagcc agaactcgta ttcaaacttt tgctgagatg
     4681 atgagtttag cgtaagttca ttaaaataaa tttactataa aatatgaaaa ttggaggaag
     4741 ctatgttata taacaaagaa caagaacttt taagaaaagc agtaagagat tttgttagta
     4801 aagaattaga tactttacca gcagaaatgg ataaaacagg tgtaatgcct aaggaattaa
     4861 tcaaaaaatt agctgatgca aaatttataa gcagtaatat cccagaagaa tatggtggtg
     4921 ggggagcagg atatgtttct tacgctatag taatggaaga aatagctaga agatgtgctt
     4981 caacagctac ttttgttaca gctggttctt ctcttgcttc attaccaata ctttacaatg
     5041 gtactgaaga gcaaaaacaa aaatacttaa aaggtatagc aacaggtgaa ttaataggag
     5101 catttgggtt aactgagcca ggagctggtt ctgatgcagg tggacaacaa acaacagcag
     5161 aattagttgg agaccactat atattaaatg gtagaaaaac tttcataact aatggaccat
     5221 tctgtgatgt agctatagta atagcagtta cagatagaag taaaggactt agaggaacat
     5281 cagcgtttat agtagaaagt aaatgggatg gtttctcaac aggagctcat gaagataaga
     5341 tgggtataag aggaactgaa acttctgact taatatttga aaacgttaaa gttccaaaag
     5401 aaaacttact tggaaaagaa ggtcaaggat ttaagatagc aatgggtact ctagaagttg
     5461 gtagaatagg tgttgctgcc ttagctctag gaatagctca aggtgcttta gatgaagctg
     5521 ttaaatatac aaaacaaaga gttcaatttg gtaagcctat agctaaattc caaaatactc
     5581 aatttactat agctgacatg gaaacaaaag tttgtgcagc tagaggatta gtttatgatg
     5641 cagcacaaaa gagagatgca ggaatgagag ttgctcaaga atctgctatg gctaaatact
     5701 atgcatcaga aattgcaaat gaagttgctt ataaagcatt acaacttcac ggtggatatg
     5761 gatttataaa agattatgaa atcgaaagaa tgtacagaga tgctagaatc gtatcaatat
     5821 acgaaggaac atcagaagtt caaaaaatgg taatttcatc aaacgtatta aaataataaa
     5881 taatctaatg aaattggagg tatagacttt gaaaatatta gtttgcgtaa aacaagttcc
     5941 agatactaac gaagtaagaa taaataaaga aacaggtact cttataagag atggagttcc
     6001 aagtatatta aacccagatg atgcaaatgc attagaggaa gctttaaaaa ttaaagatga
     6061 gcatgatgat gtaactataa cagttataac aatgggacct cctcaagcag actttatgct
     6121 aagagagtgt ttagctatgg gtgctgatga tgcaatatta ttaagtgata gagcatttgg
     6181 aggagctgat acttgggcta catcaaatac tattgcagcg ggaatatcta aagtaggtga
     6241 ctatgacata atatttgcag gaagacaagc aatagatgga gatactgcac aagttggacc
     6301 acaaattgca gagaaattag acattcctca agttacatat gtacaagatt tcaaaataga
     6361 aggaaaagat ataatagttc aaagacaatt agaagatgga tacgaattaa taaaagtaag
     6421 tacacctgta cttttaacag cagtaaaaga gttaaataca cctagataca tgtctgttga
     6481 taaaatagta aaagcatata aacatgatgt aaaagtttgg acaattgatg atttagatgt
     6541 aaataaagaa gaagttggtt taaaagcatc accaactaaa gtatttagat catttactcc
     6601 tgaaccaaga ggaaaaggag aaatcttaga aggtaaagct gatgaaatag ctagtaaaat
     6661 aataatcggt ctaaaacaaa agcacattat ataagttgga ggagtacaaa ctatgaatga
     6721 tataaaagat ttaagttctt ataaaaacgt atggatattt gcagaacaaa gagaaggaaa
     6781 aatagctcca gtagttatag aattattagg agaaggaaga aaattagcta aagaagtaga
     6841 tgcagaactt tgtgcaatat tattaggaaa agatgttgat ggattagcta aagaattaat
     6901 cacttttgga gctgacaaag tttatgttgc agatgatgct cttttagaaa aatatacaac
     6961 tgatgcatat acaaaagtaa taaaagatgc aatagatgaa ataaaaccag aaataatgct
     7021 tttcggagca actcatatag gtagagactt agcacctaga atagcttcaa gagttggaac
     7081 tggattaaca gctgactgta ctaagttaga aatagaccca gaagataaga aaataaaaca
     7141 aactcgtcca gcatttggtg gaaacataat ggctacaatc atttgtccaa accatagacc
     7201 tcaaatgtct acagttagac ctggtgttat ggataaagct gaaaaagacg aaacaagaac
     7261 tggtgaagtt atagcattag actacaaaat aactcaagat gatataagaa ctactgtttt
     7321 agaaacagtt aaaactaaga aagatttagt atctcttaca gatgcaaatg ttatagtatc
     7381 aggtggttta ggattaggtg gaccagaagg atttgaaatg cttaagaaat tagctgacaa
     7441 attaggtgga gtagttggtt cttctcgtgc ggctgttgat gctggatgga tagaccattc
     7501 tcaccaagta ggtcaaacag gaactactgt taaaccaaac ctatatatag cttgtggtat
     7561 atcaggagca atacaacatt tagcaggtat gcaatcatca gatttcataa ttgctataaa
     7621 caaaaaccca gcagctccaa ttttagaaat cgctgactat ggagtagttg gagacttaca
     7681 tgaaatagtt ccaatgctta tagaaaaatt agatagcgtt gatgatttat tagaagctat
     7741 aaaagcttaa tagatacaaa ctatataaat attactgatt gaataagtct tattagttag
     7801 taacataatt attaaccctt tgataagtta ttgctagtgt gtatttgtca gttattaaaa
     7861 agtctgtttg aataaaattt tataatacaa gataatctgg ataatcctta tatgtatatt
     7921 gcttacaaat tgtgagttat attctgtaag gattatcttt gtttt
//
LOCUS       DACTGW010000174         7905 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.174, whole genome shotgun sequence.
ACCESSION   DACTGW010000174 DACTGW010000000
VERSION     DACTGW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.174"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            69..1940
                     /locus_tag="I9573_002850"
     CDS             69..1940
                     /locus_tag="I9573_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HAT4797457.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTDTVTSKTIEIGIGDEKIDLTANPVDSTGTNLDPSTEGFRVNKIVK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISS
                     KDSETGKYKFTIKYTDASGKAIELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADVDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKADITDSQRNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            2047..2754
                     /locus_tag="I9573_002851"
     CDS             2047..2754
                     /locus_tag="I9573_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HAT4797458.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELAINKIQYVHAP"
     gene            2787..4622
                     /locus_tag="I9573_002852"
     CDS             2787..4622
                     /locus_tag="I9573_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HAT4797459.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWKKSENAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENISFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRSKHDLIDSLAVGV
                     LASKNGSPILLAGNKLDSSQKDVLNTKIIDKVIQIGGLGNENVVEDVIDMQEETKYTV
                     ETIDELNVAIKKSDANDIIRFKPEKDKNITNSFKIETNKAITIEFDGIYKQSITIDMP
                     NGDIKNFGDIDNDLVINNIKNETLVNEGSIQGIDVYDENGCKIENEDSGEIWLITILK
                     NSKDVYILNRGEITKISNNSSSVTIKNSGNINTISGSEEPSISGKKPITNDTDKDEDK
                     KARGLYPKVESCIPALKDYVRITISTKPESSKYKIYYRVVDEKPSAKYVGEKISTKGW
                     SMVTISNQSFIERAKDGSYIEAVEIDTSNNEVSRWGKSSITDDGI"
     gene            complement(4670..5065)
                     /locus_tag="I9573_002853"
     CDS             complement(4670..5065)
                     /locus_tag="I9573_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAT4797460.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIRFVFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKIFTNIVVIVLNYLFSKMIIFKK"
     gene            5292..7703
                     /locus_tag="I9573_002854"
     CDS             5292..7703
                     /locus_tag="I9573_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HAT4797461.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISIGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
BASE COUNT         3285 a          956 c         1396 g         2268 t
ORIGIN      
        1 aatatatatt ataattggta aataaggaaa aaataataaa aatttgaatt ttttaggggg
       61 aaaataccat gaataaaaaa aatctttctg taattatggc tgctgcaatg ataagtacat
      121 cagtagctcc agtttttgct gcagaaacta cacaggtaaa aaaagaaaca ataactaaga
      181 aagaagctac agaactagtt tcgaaagtta gagatttaat gtctcaaaag tatactggtg
      241 gttctcaagt tggacaacca atatatgaaa taaaagttgg cgagacttta tcaaaattaa
      301 aaataataac taatatagat gaattagaga aattagtaaa tgctttggga gaaaataaag
      361 aacttattgt aactataaca gataaagggc atataacaaa tagtgcaaat gaagtagttg
      421 cagaagcaac tgaaaaatat gaaaattcag cagacctttc cgctgaggct aattctataa
      481 cagaaaaagc taaaactgaa actaatggaa tttataaagt tgcagatgta aaagcttcat
      541 atgatagtgc taaagataag ttagttataa ctttaagaga taaaacagac acagtaactt
      601 ctaaaactat agagataggt attggtgatg aaaaaattga tttaacagca aatccagttg
      661 attcaacggg aacaaactta gacccttcta cagaaggatt tagagtaaat aaaatcgtta
      721 aactaggtgt agcaggagct aaaaatattg atgatgtcca attagctgaa ataactataa
      781 aaaatagtga cctaaataca gtttcaccac aagatttata tgatggatat agattaactg
      841 ttaaaggtaa tatggtagca aatggaacat caaagtcaat tagtgatatt tcatcaaaag
      901 attcagaaac aggaaagtat aaatttacta ttaagtatac tgatgcatct ggaaaagcaa
      961 tagagcttac tgtagaaagt actaatgaaa aagatttaaa agatgccaaa gctgcattag
     1021 aaggtaattc aaaggttaaa ttgatagctg gagatgatag atatgcaact gcagtggcta
     1081 tagcaaaaca aacaaaatat actgacaata tagttatagt taattcaaat aaactagttg
     1141 atggattagc agctacacca cttgctcaat ctaaaaaagc acctatatta ttagcatccg
     1201 ataatgaaat accaaaagta actttagatt atataaaaga tataattaag aaaagcccat
     1261 cagctaaaat atatatagta ggtggagaat cagcagtatc aaatacagct aaaaagcaat
     1321 tagaatcagt aactaagaat gttgaaagac tagctggaga tgatagacat atgacttctg
     1381 tagcagtagc aaaagctatg gggtctttta aagatgcatt tgtagtaggt gcgaaagggg
     1441 aggctgatgc tatgagtata gctgccaaag ctgctgaact taaggctcct ataatagtaa
     1501 atggctggaa tgatctttca gcagacgcta tcaaattgat ggatggaaaa gagattggta
     1561 tagttggtgg ttctaacaat gtatctagtc aaattgaaaa tcaacttgct gatgttgata
     1621 aagatagaaa agttcaaaga gttgaaggag aaacaagaca cgatactaat gctaaggtta
     1681 tagaaacata ttatggaaaa ttagataaac tatatatagc aaaagatgga tatggaaata
     1741 atggtatgct agtagatgca ttggcagcag gacctctagc agcaggtaaa ggtccaatac
     1801 ttctagctaa agctgatata acagactcac aaaggaatgc acttagtaaa aaattaaatc
     1861 ttggtgcaga agtaactcaa ataggtaatg gagttgaatt gacagtaata caaaagatag
     1921 ctaaaatact aggttggtaa taacttatac tgtggagaag attccttaat taatacagga
     1981 atctttttct ataattatgc tatttattgt aaattataac attttataaa gtaggaggat
     2041 gtagtagtgg tagatgcaac tttatttttt acaccacatc aagatgatga aaccttaagt
     2101 atgggaagtg ctataattga acatgttgaa aaaagtgata cacatgtcat attatgcaca
     2161 gatggtagta aatctataat aagaaaagtt ttggatgatg gagggtcatg ttcttatcat
     2221 attaaagatg ttcataaata ctctttatca gaaagtgaat tttcaaaaga tagagatgaa
     2281 gaatttaagg atagctgtga agcaatgggt gtaaaagaaa gtaatataca tatagaagat
     2341 aatagagcac atgatggtga gttaagtaaa gaaaaggcaa gggaaataat acttaagtac
     2401 ttagaagaat atccagatgc aaaagtaaaa actgttactc cgtttaaagc tagtggaata
     2461 catgaagacc atagggcatt aggagaagct gcactagagt tatataaaga aggcaaaata
     2521 aaagatttaa gattttatgt agaaccatac gactataagg attttaaaaa agttaatcca
     2581 aatgtagagg tatggaaggt attacctagt caagaggaga agttattaag tgcaatgaat
     2641 gcatataaaa aatggaatcc tgaaagtgga cattatgcta taggatatca ttcagtaaaa
     2701 tctcattttg atgagctagc tataaataaa atacagtatg tacatgctcc ataaaatatt
     2761 atgaaagaat taggaggtaa gaattaatga aaatatcaaa aaagatagtg tctttgttaa
     2821 ctatgacatt tttaactgtt acattatatg gaaatacatc taatgcatct acaaaagata
     2881 cattaacagg ttctggaaga tgggaaacag caataaaaat aagtcaagca ggatggaaaa
     2941 agtctgaaaa cgctgtgcta gtaaatgata attcaatagc agatgcttta tcagctactc
     3001 catttgctaa agcaaaagat gcaccaatat tattaactca aagtaataaa ttggatagta
     3061 gaacaaaagc agaattaaaa agacttggtg tgaaaaatgt atatttaata ggtggttcaa
     3121 ttgcattaag ttcagagatt gaaaagcagt taaatgcaga aaatataagt tttgaaagaa
     3181 tatctggaaa tagtagatat gatacttctt taaaattagc tgaaaagcta gatagggaaa
     3241 aatctatatc taaaatagta gtagtaaatg gagaaaaggg tcttgctgat gcagtgagtg
     3301 ttggagctat agctgctcaa gaaaacatgc caataatact ttctgattca gagaatggaa
     3361 ctgaagtagc tgataatttt atagatagta aagatataga aaaatcgtat gtaataggtg
     3421 gtacatattc tatttctaat tctgtagaaa gaagtttacc aaatgcaaca agaatagcag
     3481 gtagtagtag aagtgagaca aatgcaaaaa ttatagaaga attttacaaa gatactgata
     3541 taaaaaatat ttatgtaaca aaggatggta caaggagtaa gcatgattta atagattctt
     3601 tagctgtggg tgtattagct tctaaaaatg gttctccaat acttttagca ggaaataagc
     3661 tagatagttc tcaaaaagat gtgttaaaca ctaaaattat agataaagtt attcaaattg
     3721 gcggattggg taatgaaaat gttgtagaag atgtaataga tatgcaagaa gaaactaagt
     3781 atactgttga aactattgat gaacttaatg ttgctataaa aaaatcagat gcaaatgaca
     3841 tcataagatt taagccagaa aaagataaaa atataactaa ttcttttaaa atagaaacaa
     3901 ataaagctat aactattgaa tttgatggta tatataaaca atcaataact atagatatgc
     3961 caaatggaga tataaaaaac tttggggaca tagataatga tctagtaata aataatataa
     4021 aaaatgagac tttagttaac gaaggaagta tacaagggat agatgtttat gatgagaatg
     4081 gatgtaaaat agagaatgag gattctggag aaatatggct tataacgata cttaaaaatt
     4141 caaaagatgt atatattttg aatcgtggag aaataactaa aatatctaat aattcttcta
     4201 gtgtaacaat aaaaaattca ggtaatatta atactataag tggaagtgaa gaaccatcaa
     4261 taagtggtaa aaaacctata actaatgata cagataaaga tgaagataaa aaggcacgtg
     4321 gtttatatcc taaagtagaa tcttgtatac ctgctttaaa agattatgta agaataacta
     4381 tatcaacaaa accagagagt tcaaagtata aaatttatta tagagtagtt gatgaaaaac
     4441 catctgcgaa atatgttgga gaaaaaatta gtactaaagg ttggagtatg gttacaatat
     4501 caaatcaatc ttttattgaa agagccaaag atggatcata tatagaagca gtagaaatag
     4561 atacctcaaa taatgaagtt tcacgatggg gaaaatctag cattacagat gatggaattt
     4621 agacattaaa cttattcatt ttgcagatta ctatttagta atctgctatt tattttttaa
     4681 aaattatcat cttgctaaac aagtaattta aaactataac aactatatta gtaaatattt
     4741 ttacaataac atcatttaca aaaagcaaat caatcattac atacataatt aacatttcaa
     4801 cgacaccaga aagtagccta aaactaacaa aagacaaaaa ttctttaaat acgaacctta
     4861 tttcaattct tttactttca aatacaaaaa acttattagt aacataagca aataaaacag
     4921 caagtatcca tgccaaagca tttgcaacca taaaattaaa taaaataact ctagtaaaaa
     4981 ataaatacga aactatattt acaagagtag taaatgctcc aaaaaataaa tataatattg
     5041 tctctctatg cttttttaaa atcaaatata atcctcctaa tctaatttaa tataaatata
     5101 cttaaagata ctaacaaaca ttagtatagc acatctatta ataaatcaat atatcttaaa
     5161 atattataaa aataaaacta taatattcta taattaatat gtactcataa tccatatttt
     5221 atcaaaataa aatatgacct taatttagaa atctgatata ataaaaatta aatacatata
     5281 agggggtaaa catgagaaag tacaaatcaa aaaaattgtc aaagctactg gcattgttga
     5341 cagtttgctt tttaatagtg tcaacaatac ctgtctcagc agaaaaccat aaaactctag
     5401 atggagtaga aactgcagag tattctgaaa gttatcttca atacctagaa gatgtcaaaa
     5461 atggagacac agcaaaatat aatggagtaa taccttttcc acatgaaatg gaaggtacaa
     5521 cacttcgaaa taagggcaga agtagtcttc catcagcata taaatcaagt gtagcataca
     5581 acccaatgga tcttggtctt actacaccag caaaaaatca aggaagtctt aatacatgct
     5641 ggtctttttc aggtatgtca actttagaag catatcttaa attaaaggga tatggaacgt
     5701 atgacttatc agaagaacat ttaagatggt gggcaactgg tggaaaatat ggatggaact
     5761 tagatgatat gtcaggtagt tcaaatgtaa cagccatagg atatctgaca gcatgggcag
     5821 gtcctaagtt agaaaaagat ataccatata atcttaaatc tgaggcacaa ggtgcaacta
     5881 aaccttcaaa tatggatact gcacctactc aatttaatgt aacagatgtt gttcgtctta
     5941 ataaagataa ggaaactgta aaaaatgcta taatgcaata tggttcagtg acatcaggtt
     6001 atgcacatta ttcgacatat tttaataaag atgaaacagc atataactgt acaaataaga
     6061 gggctccatt aaatcacgct gtagcgatag taggatggga tgataattat tcaaaagata
     6121 actttgcatc tgatgtaaaa ccagaatcaa atggagcatg gttagtaaaa agcagttggg
     6181 gagaatttaa ttctatgaaa ggattcttct ggatttctta tgaagataaa actctactaa
     6241 cagatacaga taactatgca atgaaatcag tatcgaaacc agatagtgat aaaaaaatgt
     6301 accaacttga atatgctggt cttagtaaga taatgtcaaa taaagtaaca gcagcaaatg
     6361 tatttgattt tagcagagat tctgaaaaac ttgactctgt tatgtttgaa acagactctg
     6421 taggagcaaa atatgaagta tattatgcac cagtagtaaa tggagttcct caaaacaact
     6481 caatgacaaa acttgcaagt ggaacagtat catattctgg atacataaat gtacctacta
     6541 attcttacag cttaccaaaa ggtaaaggag caatagtagt agttatagac aacacagcaa
     6601 atcctaatag agaaaaatca actttagcat atgaaactga tatagatgga tactatctat
     6661 atgaggctaa agcaaactta ggtgaaagct acatacttca aaacaataag tttgaagata
     6721 taaatacata tagtgaattt tctccttgta actttgttat aaaagctata acaaaaacat
     6781 cttctggaca agctacttca ggagaatctt taactggagc agatagatat gaaacggcag
     6841 ttaaagttag tcaaaaagga tggacttctt cacaaaatgc agtattagta aatggagacg
     6901 caatagtgga tgctttaaca gctacaccat ttacagcagc aatcgactct ccaattcttt
     6961 taacaggaaa agataactta gattcaaaaa ctaaggcaga gttacaaaga ttaggaacta
     7021 aaaaagttta tctaataggt ggagaaaatt ctttaagcaa gaatgtacaa actcaactta
     7081 gtaatatggg tatatcagta gaaagaattt caggtagtga tagatacaag actagtatat
     7141 ctctagctca aaagttaaat agtataaaat ctgtttcaca agttgcagtg gcaaatggtg
     7201 taaatggact tgcagatgca ataagtattg gtgcagcagc tgctgataac aatatgccaa
     7261 taatacttac taatgaaaag agtgagttgc aaggtgctga tgagttttta aattcatcaa
     7321 aaataactaa gtcttatata attggtggta cagctacttt atcatcaaat ttagaaagta
     7381 agctttcaaa tccaacaaga cttgcaggaa gtaatagaaa tgaaactaat gctaagataa
     7441 tagataaatt ctaccctagc tcagatttga aatatgcttt tgttgttaaa gatggttcaa
     7501 agagtcaagg agacttgata gatggactag cagtaggagc attaggtgct aaaactgatt
     7561 caccagtagt tctagttgga aataagttag atgaaagtca aaaaaatgta cttaagtcta
     7621 agaaaataga aactcctatt agagttggtg gaaatggaaa tgaaagtgct tttaatgaac
     7681 taaatactct tttaggaaaa tagtgaatgg aaagatagta aggatataca ttttattatt
     7741 aaaaaatatt caatagaagt ttataaatat aaaataacaa aaaggggtgt ctcaaaatga
     7801 actttttagt tcatgaggca ctcttttttg ttgttttata tttttatcat ttaaataatg
     7861 aaaaataggc ttgtctatat ttttagatag ttttcttatt attga
//
LOCUS       DACTGW010000175         7897 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.175, whole genome shotgun sequence.
ACCESSION   DACTGW010000175 DACTGW010000000
VERSION     DACTGW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.175"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            36..497
                     /locus_tag="I9573_002855"
     CDS             36..497
                     /locus_tag="I9573_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT4797462.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            565..2811
                     /locus_tag="I9573_002856"
     CDS             565..2811
                     /locus_tag="I9573_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797463.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            2804..4636
                     /locus_tag="I9573_002857"
     CDS             2804..4636
                     /locus_tag="I9573_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797464.1"
                     /translation="MSRMGRGPMGKSMEAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            5361..6632
                     /gene="hflX"
                     /locus_tag="I9573_002858"
     CDS             5361..6632
                     /gene="hflX"
                     /locus_tag="I9573_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAT4797465.1"
                     /translation="MTKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSITQN
                     AKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRTAL
                     ILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKLEL
                     DRRRIEEKITSLNRELYDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLVDI
                     FKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRSTL
                     EEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVLDR
                     ILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDCVRCKFLIPYDKGHVVSYFNENTS
                     IINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            6865..7362
                     /locus_tag="I9573_002859"
     CDS             6865..7362
                     /locus_tag="I9573_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT4797466.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            7412..>7897
                     /locus_tag="I9573_002860"
     CDS             7412..>7897
                     /locus_tag="I9573_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAT4797467.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKDLQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KE"
BASE COUNT         3124 a          818 c         1450 g         2505 t
ORIGIN      
        1 ccgactaagt aatataaaaa ataaggaggg caagtatgaa tagcaattct gaagaaaaat
       61 taaatgatgt tttcttgcaa ttaatgcgaa tatattttat aagaactcat ttttattttg
      121 ataaattagg cttatatcat ggtcaacccc aaatactttt ttctttaaag atgaaagatg
      181 ggcaaagtca aaaagaatta gcagataaaa ggaaagtaaa agcatctaca actacagtta
      241 tgataaagag gatggaaaaa gctggacttg tggaaagaaa acaagatgaa aaggaccaaa
      301 gagtatctag gatttttttg acagacaaag gtttagaaat atgtaataaa gttgaagaat
      361 ttaacaagga attggaaaga gagttttttt taggatttag tgaagaagaa aaaatagtac
      421 ttagaagact tctaatgcaa gtacgagata atttaaaagc aggatgtcat aagcatgaag
      481 atgaaaaata tttttaaagt ttaaaataat gtttttcaat ttaatgttat tagactaatt
      541 aatattacag aagggaagaa atgtatgttt aaattaatta aatatttaaa gaaatcggca
      601 ttatcaatag taataatagt ctgtctattg gtaattcaag cggtatgtga cctttcttta
      661 ccagaataca catccaatat agtaaatgta ggtatacaac aaggtggagt agaaaactct
      721 gttccatctg tgattagaga gagtgaatta aataaaatta ctttatttat ggataaaagt
      781 agtaaggata aagtactaga taattatact ttgctgaata aaaaggatta tgttaagtat
      841 aaagataagt acccaggtct aaaagatgaa tctctttatg agttaaatac taaagacaaa
      901 gacactatag atgatttaaa tgttattttt ggtaaagcta ttttaatagt atcagggctt
      961 gagggcgata gtaaagaggt aaaagctatg aaagctcagt taatgagtaa acttccacca
     1021 caagctactc aaagtggaga tgttgatata tttaaattgt tatcagcaat gccaaaggaa
     1081 cagttagata cattgactaa agagatgagc aaaggctttg aaagtatgcc agagagtatg
     1141 ataacacaaa gctctgtttc atatgtaagg tcagaatatg agaaaatagg tattgataca
     1201 gaaaaaactc aaaataatta tatattattt actggtgcta aaatgcttgg tatagcaatg
     1261 attagtatgg tagctacaat aacagtagga tttttagcag caagagtagc agcaagtgta
     1321 ggtagaaatt taagaagtgg agtatttaga aaagttatgt cattttcaaa tactgaaatg
     1381 aatgaatttt ctacagcttc attaattact cgtagtacaa atgatattca acagatacaa
     1441 atgttgatgg ttatgttgct tagaatagta ttctatgcac ctataatggc tataggtgga
     1501 gtgataaagg ttttaaacac aaatacttca atggcctgga ttatagcagt agcagtagtt
     1561 gctatcttat cattaattgt agtgttattc tcagtggtta tgcctaagtt taaactggtt
     1621 caaaagcttg ttgataagtt aaacttagta agccgtgaaa taattactgg tattccagtt
     1681 atcagagctt ttagtactga aaaacatgaa gaagaaagat ttgataaagc aaataaaaat
     1741 ttaactaaaa caaatctttt tgtaaataga gttatgacat gtatgatgcc tgctatgatg
     1801 cttataatga accttatcac agttcttata gtttggagag gtgcatacag tgttgattct
     1861 ggagcaatgc aagttggaga tatgatggca tttatacaat atacaatgca aataataatg
     1921 tcattcttaa tgatatcaat ggtatctatt atgcttccaa gagcatccgt ttcagctaca
     1981 cgtatagatg aagtattagt gtcagatata agcataaaag acccagaaaa atcagaggca
     2041 caagagttta aggaagataa aaagggtctt gttgaattta aaaatgtttc gttcatgtat
     2101 ccagacgcag aagaacctat attaacaaat atatctttta cagcaaagcc aggagaaaca
     2161 actgccttta taggaagtac tggtagtggt aagtcaactc ttataaattt aattcctcgt
     2221 ttctttgatg ttactgaagg agaaatatta cttggtggag ttaatataaa aaatgtttct
     2281 caacatgatt taagggaaaa aattggatat gtacctcaaa agggagtatt gttttctggt
     2341 actatagaat ccaacttaaa atatggtaga aaagacgcaa cagatgaaga aatgagaaca
     2401 gcagctgaaa tagctcaagc tacagaattt atagattcta aaccagaagc ttttaaaact
     2461 gaaatttctc aaggtggaac aaatgtatct ggaggtcaaa aacaaagact ttctattgca
     2521 agagcaatag caaaaaatcc agaagtttat atatttgatg atagtttttc agcacttgac
     2581 ttaaaaactg atgcagctct tcgtaaggct ttaaaatcaa aaactgctga aagtacagtt
     2641 ctaatagttg cacaaagaat tagtactatc ttaaatgcag aacagataat tgttttaaat
     2701 gagggagaaa tagttggaat aggaacacat aaagagcttc taaaaaattg cgaagtttat
     2761 gagcaaatag ctttatcaca actttcaaag gaggagctag ctaatgagta gaatgggaag
     2821 aggtcctatg ggaaaatcta tggaagcagg acaaaaagca aatgacttta aagggactat
     2881 gcgaaaacta attgcatatt tatctaaatt taaaatatca ataattttag taattgtatt
     2941 tgctattggt agtgcatcgt tttctatagt aggtccaaaa attttaggaa aggcaactac
     3001 aaagatattt gaagggttag tgagtaaagt ttctggagga aacgtaggta tagattttaa
     3061 tgcaattgga aaaatcttaa cctttttact tttcctttat ttgataagtg cactgttctc
     3121 ttttatacaa ggatttataa tgagtggaat ttctcaaaaa gtatcctata atttaagaaa
     3181 agagatttca gcaaaattgg atagattacc aatgaagtat tttgatacta agacgcatgg
     3241 tgaaatatta tcgagaatta cgaatgatat agatacttta aatcaaagtt taaatcaaag
     3301 tatgacacag cttataacat cagtaacaac tatgataggt gttttgataa tgatgttatc
     3361 tataagtgga attatgacat tagtggcagt tttaatatta ccaatatcaa tgtttgtaat
     3421 ttcaagaata ataaagaaat cacaaaaata ctttagatat caacaagaat atttaggaaa
     3481 tgtaaatggt caagttgagg aaacttatag tggccaaaca atagtgaaag catttaatcg
     3541 tgaagatgaa gtaattgaag agtttgataa actaaatgat agcctgtata attcagcgtg
     3601 gaaatctcaa tttttatcag gtataatgca accacttatg atgtttattg gaaacttggg
     3661 atatgtaatg gtatcaatct taggaggttg gcttgcaatt aagaaaacaa ttgaggttgg
     3721 tgacatacag tcatttatac aatatgtaag aaactttaca cagccaatga ctcagattgc
     3781 acaggttgcc aatttattgc aatcaacagc agcagcttct gaaagagtat ttgagttttt
     3841 agaagaggaa gaagaagtcc aaattgttga aaatgcagtt tcaatagatg gtttagaagg
     3901 taaaattgat tttgaaaatg tcaactttgg atataaccca aacaagacta taataaatga
     3961 tttctcagta aatgttaaac ctggtcaaaa agttgctatt gttggaccta caggagcagg
     4021 taaaactaca atagttaaac tgttaatgag attttatgat gttaatagcg gttcaatact
     4081 gattgatggt cataatatta aagattttaa tcgtagtgag cttagagaaa tgtttggtat
     4141 ggttcttcaa gatacatggt tatttagtgg ttcaattatg gaaaatataa gatatggtaa
     4201 gttgaatgct acagatgaag aagttataga ggctgctaaa tctgcacatg ttcatagatt
     4261 tattaagacg ttaccagatg gatataagat gaaattaaat gaagaagcaa gtaatgtttc
     4321 tcaaggtcaa aaacaattat tgactatagc tcgtgctatt ttagctgatc ctaaaatttt
     4381 aatattagat gaagctacaa gttctgtcga tactagaaca gaagtattga ttcaaaaagc
     4441 tatggataat cttatggaag gtagaacaag ttttgttata gcccacagat tatcaactat
     4501 acgtgatgct gatatgattc ttgtaatgaa tgaaggagat atagttgaac aaggaaatca
     4561 tgaggaactt ctaaagaaag gcggattcta tgccaatctt tacaattctc aatttgaaga
     4621 agatgaagca atgtaacaaa aaactatctt agcatagaca tgtaattaat aagataaaat
     4681 aaatgagggt gccttaaaat gaacttttta attcataagg cactcttttg tgtatgaaat
     4741 aaagtgtatt tttatcattt taacaatgaa aaagaggatt atctatattt tgagatagcc
     4801 acataatttt atgtctttac aaagtgttta ttttattata atatgtagta aggaaaatgt
     4861 tggaggtgat tatcaaaatg tatatttaat tttattcttt aaattttatt ataaatagaa
     4921 tttaatttta atagttgatg attatattga agtatatgtt tttattactt ttattaatca
     4981 tgcaaataac tacttaaatt taagaatagg gttaaatata aaatgataat tacatataag
     5041 ttaattaaac taatgtattt ttaatatata tttagaattt taatccttct tatgatataa
     5101 gtagcttgtt ttgtattact tagatttgta aggagttttt ttattgaaaa aactgcataa
     5161 tatatttggg aggaggtgta agaaagtgat tttttatata agattgagaa aataatgcac
     5221 gattatggtt attataatag taaataagaa gtgtcattta aggataagat aaaaagcaaa
     5281 attcttgatg aatttaatta tagagccata atctcgtgct atagattaaa taaaaaatag
     5341 cagataagga gaatatggat atgacgaaaa aaggaataac agtaggatta aacataaata
     5401 ataaatctga ggactttaat gaactaatga ttgaacttga aaacttatgt tcagcatgtg
     5461 atattgatgt tgtagggagt ataactcaaa atgcaaagca ggttaataga gcattttaca
     5521 ttgggacagg taaagttgag gaaatattaa acttaataaa gaaagaaaac atagagatag
     5581 ttatatttta caatgaactt tctacatcac aactaaaaaa tcttgaagaa aagcttaatt
     5641 gtgaaattat agatagaacg gcattgatac ttgatatatt tgcacaaagg gcaaaaacta
     5701 gagaagctaa acttcaagta gaagtagcaa gtttaaaata tatgttacca agacttatag
     5761 gttcaaatga aaatttagga cgtcaaagtg gtggagttgg tactaaaaat agaggttctg
     5821 gtgagaaaaa attagaactt gaccgtagaa gaatagaaga aaagataaca tctttaaata
     5881 gggagttgta tgatttaaaa ttccaaagag aaactcaaag aagtatgaga agaaaatcaa
     5941 atcttccaaa tgtagctcta gtcgggtata caaatgctgg taaatcttca ataatgaata
     6001 aactagtaga tatatttaaa aactctgaag aaaagaaagt gtttgagaaa aacatgttat
     6061 ttgctacact tgaaacttct gtcagaaata tagttcttgc aaataacaaa gaatttttat
     6121 tatctgatac tgttggtttt gtaagtaatc taccacatga cttagtaaaa gcatttaggt
     6181 caacattaga agaagcatgt gaggcagacg tactgttaca tgttattgat atttcaaatc
     6241 caagctataa aagtcatatt aaagtgacag aagatacttt aaaacaaata ggtgcagatg
     6301 gtatacctat gatacatgtg tataataaaa ttgatttaat agatgtagaa gtattggata
     6361 gaattttaga tagtatcgat aaagaaggta tatttgtttc agttaaaaaa gatataaata
     6421 tagataaaat gataaaatgt atttgtgata gcatatttaa agattgtgta agatgtaagt
     6481 tcttaattcc atatgataaa ggtcatgtag tttcttattt taacgaaaat acgagcatta
     6541 taaatacaga atacagagaa gatggagcta ttttagatgt agaatgtagc aatatagaat
     6601 ataataagta taaaaagtat gctttagagt aaattttgaa aaacttatta tagcctaaaa
     6661 tatatttaat ttataattta ttataaattg attgtaaata tgattttatg aaacggtata
     6721 gccataatgc tatgccgttt tgccatttat aataaaaata aatttattga cacaatagta
     6781 actaattact ataatataaa gtaactagtt actatttaaa attatcaaaa aattaaaatc
     6841 taagataaag agaagtgtat aataatggat gcagaaagtt taatgaaaaa atatatggta
     6901 gatgatgata gtagaaaaca agctatattt gcaagtatat ttgtaatgca aaataaactt
     6961 caaactacat gtgataaatt agacccagaa ttaacaatga aacaatggtt acttttagct
     7021 attgcggact ctgtagatga aactcttaca ttaaccaaac ttggagaatt aatggggtgc
     7081 tcaagacaaa atgtaaaaaa actagctatt gctttagaaa agaaaggttt tatacaaatt
     7141 gagcaaaatg aaaaagattc aagagctatc tgtcttgtga caaaagatag agtgcaagag
     7201 tattctaaaa aagtaagagg tatgcaggaa aaagtattac agcttttatt tgaagatttt
     7261 acagagaaag aagtggaaca gttatttttt ggaattatga aattaagtat aggaattgaa
     7321 aatgtgaaaa attatgtaga aaataatgac gttggagaat aagcaagtat caaatcaact
     7381 ttgtatgaca tcagagaggg gggtaattta tatgaaaagt gtagtagtga tttataagag
     7441 taagtatggt tcaacaaaaa aatatgctaa atggctttca gaattattgt cttgtgatat
     7501 ttttgaaact aagaatatta atatagaaaa ggttatgaag tatgatacga ttattttagg
     7561 aggaggtctt tatgcttgta atatagcggg aatttctttc ttaaaaaaaa attataaaga
     7621 tttgcagaaa aaaaatttag tagtatttgg cgtaggagct gctccatatg atgatgaagt
     7681 aataaagaaa ttaagagaag taaactttaa ggatgaatta aacaaagttc cttgctttta
     7741 ttgtcgtggg gcatgggatg agacaaaaat gtcttttata gattatactc tttgtagttt
     7801 ttttaaaaaa tctatagcta aaaaagaacg tcaaggtctt aaaccagcag aagcaccttt
     7861 attgtctgga gaagcctttg attggtgtaa taaagaa
//
LOCUS       DACTGW010000176         7879 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.176, whole genome shotgun sequence.
ACCESSION   DACTGW010000176 DACTGW010000000
VERSION     DACTGW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.176"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(36..413)
                     /locus_tag="I9573_002861"
     CDS             complement(36..413)
                     /locus_tag="I9573_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAT4797468.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(413..799)
                     /locus_tag="I9573_002862"
     CDS             complement(413..799)
                     /locus_tag="I9573_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAT4797469.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            978..1733
                     /gene="nadE"
                     /locus_tag="I9573_002863"
     CDS             978..1733
                     /gene="nadE"
                     /locus_tag="I9573_002863"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HAT4797470.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYRAEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            1816..2535
                     /locus_tag="I9573_002864"
     CDS             1816..2535
                     /locus_tag="I9573_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAT4797471.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(2703..3707)
                     /locus_tag="I9573_002865"
     CDS             complement(2703..3707)
                     /locus_tag="I9573_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAT4797472.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            3973..4869
                     /locus_tag="I9573_002866"
     CDS             3973..4869
                     /locus_tag="I9573_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HAT4797473.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            5007..6269
                     /locus_tag="I9573_002867"
     CDS             5007..6269
                     /locus_tag="I9573_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797474.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            6345..7538
                     /locus_tag="I9573_002868"
     CDS             6345..7538
                     /locus_tag="I9573_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HAT4797475.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LKRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
BASE COUNT         2879 a         1018 c         1447 g         2535 t
ORIGIN      
        1 ttaagtgaaa agccattaaa ttgatttaat ctaaatcaat ttaatggctt tttatcggat
       61 tttgcacaga atcctttttt tacttcaaat aaaccgacta aaagtaaaaa tatcccaaat
      121 atttttttta atatatctga agacagataa ttagctaata gtgaaccaaa tacagctcct
      181 aaaaccccaa acacagctat aggtataacc aatttaaaaa ttacattttt attttttata
      241 tgtatagcaa gtgctataag tgtcataggg atagatgaaa gtaaatttac gctttgtgct
      301 attttagtgt ctatgccagc aagtaaaacg agtgcaggta taaggattgt tcctccaccc
      361 attcccattc ctccaataat tcctgcaaaa aatccaatta gagcaaataa cattaaaaca
      421 ccatccttaa tgctgcaatt atcataatta ttccaaaaga aattctaaga aactttccag
      481 taaccttatt tagtaattta gctcctataa taccaccaag aatacttcca actgcaactt
      541 taattgttaa gttaacatca taagtacctt tagatacata taaaacagaa cttgctgtcg
      601 ttaagaatat tattatagaa agagcagttg catgagattt atgttcttct accttaacga
      661 tgtcatttaa tattggaact aaaagagtac ctcctccaga accaaaaact ccatttataa
      721 atcctgtaaa aactccaatt atcgtatttt ttaagttaaa atcaaagtat tttttcttta
      781 aatcatcagt ttttttcata aaaatcacct tctgaaatag ttttaccaaa ataggcaaat
      841 tatatttata taaaataaga ttaaatataa tctttcatgt ttgtttaatt ttgcataaaa
      901 aaaaacatat taaaacaaca tattaatata tggtataata tattaaaaca aagtagcaaa
      961 tttgagagga gataacaatg tcaaatataa aaatacaaat agacaaaact gtagaatggc
     1021 ttataaataa ggttaatgag gcaaatgcaa aaggattaat tgttggtgta tctggaggaa
     1081 ttgattcggc agtagttgct aatttaatta aaaaggcatt tccagaaaac tctatgggag
     1141 ttataatgag cataaaaagt aatcctcaag atagagaaga tgcacttaag gttatagagg
     1201 gatgtgatat agaatacctt gatttagacc ttatagaacc acaaagtgct atattagaca
     1261 tggtagtagg aaatttaaaa gataaacatt tatatagagc agaatattta aagatgacag
     1321 atgcgaactt aagagcaaga gtaagaatga gtacaattta tacaattgct aacaatctag
     1381 gatatttagt tgttggtaca gacaatgctg cagaaataca cacaggatat tttactaaat
     1441 ttggtgatgg tggagttgat attttaccta ttgcaaattt aactaaagga gaagtatatg
     1501 aatgggcaaa agagcttggt gttcatgaag atttaataaa caaagctcct tcagcaggtc
     1561 tttgggaagg tcaaacagat gaagatgaaa tgggaactac ttacaatatg atagatgctg
     1621 tgttagaagg tagattggat gaagttccta aaagagacca agagataata gaaagacttc
     1681 atagattaag tgaacacaag agaaaaacgc ctgcacaacc tcctaagttc taatttacta
     1741 tttataagat ttgtgataaa atttacaagt gatgaaaatt agaataatta attgtacgta
     1801 ttaaggagtg gaaatatggg acgtataggt aatataatta atagaaaagg taaacaagat
     1861 gctcaaagag caaagatatt tactaaacat gctagagcaa tagcagttgc agctaaagaa
     1921 ggtggagcag acccagagta caatgcagcg ttaaaaacag ctatagaaaa agctaaggca
     1981 gataatatgc caaatgataa tatagataga gctatcgcta aaggtgctgg agcaggcgct
     2041 ggagaagact atgaaactat agtttatgaa ggatatggac ctggtggagt tgctgttata
     2101 gtagaaactt taactgacaa caaaaacaga actgctggta acgtaagata ctattttgac
     2161 aaaaacggag gaaacttagg aactagtgga tgtgtgtctt ttatgtttga taaaaaagga
     2221 caaatattag ttggtttagg agatggagtt tcagaagaag aattgatgga tgttgcatta
     2281 gaagcaggag ctgaagattt cataacagaa gaagatggtt atgaaataat aactacacca
     2341 gaagattttt ctagtgttcg tgatgagtta aaagctaaag gatatgagtt tatatctgct
     2401 gatgtgaaga tgatacctca aactacgact gtattgactg aagaatctca tttaaaaatg
     2461 atgaataaat tagttgatat gcttgaagaa gatgatgatg ttcaagatat atatcataac
     2521 tgggaagtag aataaatagt ataagcatat tgaaatttat aagcattgaa tcaattttaa
     2581 catgtgataa attttataga ataagaaatt actaaaatag acatacgcac ctctaaccta
     2641 tatttgcata ataatatgta agtataggta aggggtgttt ttatatttat aggttaagaa
     2701 atttaaagta aattttgagc tggacttgat tgtctataaa taattgatgg gtatataact
     2761 tgttgctgaa taccatttat acgcccagtt actgtatcaa taatcatcct aagactacgt
     2821 tctgctgtct cttccatttt taaatctata actgtcaaag gaggaatact tcttgccatt
     2881 agtcgtgaag gtccattact tgctgcaaat atttgaatat cctctccaat tacaattcca
     2941 gattcatgaa atgcattcat cataccaaga gctacaacat catgattaca aaaaattact
     3001 ttttttagtg aattttttgc aagcattttt tgagcaatat catatccact atctatttta
     3061 ttagaacaat aatatataga atctgataga tgtattccat gttctttaca tgtttttaaa
     3121 aaagatttac cccttaaagt catacatgta aaggaagttg ggttgaatac aacagcaata
     3181 tcatttttac cattttgaat agcatgtaaa gctgctatac gaccagtttc ctcattatca
     3241 atagatacac ttgaaaaacc aggtatttca cgatttacaa caactacagg aattttaggt
     3301 ttatttttta ctagatattc tatatcatca gcattgatac ctgttaaaac aattccatca
     3361 taataattat ttgcaaaaaa gtattcatca tcacatagat gaccaacttt gtaaggtcgt
     3421 atgatgatat ttatgtttaa atgttctaaa gaaattatgt ggtttattgc ttgaagtata
     3481 ttggttgatg ccaactcaaa tccctcatcc tgccaataaa aagctatttt aatattatta
     3541 tgtttaatag gagtagagcg tttatatcca agcttatcag ctgaatttaa tataagctct
     3601 tgtgtatgta gcggaatcct gcgttcattt cctttaccac ttaagacgta cgagacagtt
     3661 gctgaagaaa gaccacagtc ttttgcaata tctttgattg ttgccatgat aaaatcctcc
     3721 ttaaatagtt gcttaaattt gcttatacca ataatcttaa catatattca actaaagaac
     3781 aatattctac aaggtgcata ttttacagga ttagaataaa taaatttaaa aaaacttctt
     3841 acttatgttg acataattta gtttatagga ttataatatg cttaaataag cttattatta
     3901 actcaaatta agcaaagtta agcaggaaca tgcaatgcta gcttgcaatg tgactgtaaa
     3961 gggggaaaag gtatgagtaa ggtaaatata tttgaagttg gtcctagaga tggatttcaa
     4021 aacctgaaag agtttttaga aactgataaa aagcttaaga ttattgatgg tcttattgct
     4081 tcagggatta aacaacttga aattagttca tttgtaagcc caaaagcaat acctcagatg
     4141 aaagatgcaa aagaagtagc aacttattgt gtagaaaaat atcctgatgt gaaactttat
     4201 gcattagttc caaatttata tggtgctaaa gttgcatggg agtgtggtat aagagatata
     4261 tcctatgtta tatctttgag tgaaactcat aataaaaaaa atataaatcg tacccatgaa
     4321 gagtctattg atgagcttaa aaaaataatg aatacttatc cagacatgaa tatatgtatt
     4381 ggaatggcta ctgtatttgg gtgtcctttt gaaggtttac caaaaattga agatgccata
     4441 agatttgcta aacgcctttg ggaaaacggt atcagggaga taaatctagc agatacaata
     4501 gggattgctg accctaagca agtaagagaa actattagtg cattagttga agctttacca
     4561 gagtgcagat ggcaggtaca tattcatgat actagaaata tgggaatggt taatactctt
     4621 gtggctattg aatctggaat tactactgtt cagtctacac ttggaggatt aggaggatgc
     4681 ccatttgctc ctggagcatc tggcaatact gcaactgaag atttagtata tatgcttgaa
     4741 cgtatggggt atgatacagg agttgatttt gttaaacttc ttgaattatc aaagtatcag
     4801 aagagttttg taaatggagt atatagtggt catcatgtaa atataaagtc tgatagttgt
     4861 aattgttagt tctattgatt aatcgattaa aaaaatatgt tgattaaaaa aatatgctga
     4921 ttggtaaaat agatttattg ataaaaaaga taaattataa aatacattat ctaataaaac
     4981 aaataaaaaa attgaggggg attagtatgg atattatagc tttactttta tttttaggag
     5041 ttattattat tgcatttgta cgtaagaata atgcaggtat tttggctcta gctattggtg
     5101 tcatagctgt tagaattttt ggaatggatg ataaagaact ttatggagct ataagcaact
     5161 caatgtttac tacacttgtt ggtataacac tactctttgc aatagtgaac tcaacaggag
     5221 cattaaagct tgttgcagaa aaaattgtag ctgcttctgg taaacgtgtt tggcttatac
     5281 ctatagcagt ttttatagca ggatttgttg ttgctggtgt tggacctggg gcaattccag
     5341 cacttgcaat tatcccagca atggctgttc ctgtagccat aacggttggt tataatcctg
     5401 taatgcttgc acttataggt gaatgtggat taatggcagg tcgtatgact cctattactc
     5461 cagaaggtca aattataaca accgttgcag aaggggttgg aattgaaaat gttatgccta
     5521 ctatcttagc ctgtcaaact ctttctacaa ttgtattttc aatagtattg tttataattt
     5581 tcaaaggata taaacttaaa aaacctataa atgttataga tagtaagcaa atagaaaaat
     5641 ttaatataaa gcagataatt tctctaataa gcatagtagt tatgattgct tttatcataa
     5701 tttttgatat gaatgttggg cttgcagctt ttgcagtttc agctgtactt atttttttca
     5761 atattgctga tgaaggagaa tgtatcaaat caattccatg gagcacgata gtaatggttc
     5821 ttggagtagg tgcaatgatg acaatcgtag atgcagcagg aggtattgac ttgatgagta
     5881 atgcattatc atcactgatg aatagtaaaa cagctactcc aattatgagt atttcagcag
     5941 gacttatgtc tatggttagt tctgctttag cagtagttta tccaactatg atgcctatgt
     6001 gtgttgatat agctaaggca gttggaggag tagacccact agcacttatt gctgcagtcg
     6061 gtgttggagg ttctcttgct ggtgtaagcc cactttctac tggtggggca ttgattttag
     6121 cagctatggg ttcaagtaag aaagacttta ataaggaaga acaaaataag gtttttgtac
     6181 agcttttcat tatgagtgca gttggcttat tggttattgc agtagtatca atattatcat
     6241 ttaatgcaat aacacattta atgaactaat tataataact gaaaataatt cattaaattt
     6301 aatttttaat attaatcaat ataatataat ttggagggga agttatgaaa aaaggagcat
     6361 taagcaatat aaaggtactt gaccttacaa gagttcttgc aggtccatat tgtacaatga
     6421 tgttggcgga ttttggagct gatgttataa aaatagagat gccaagaaaa ggtgatgata
     6481 caagaggttt tggacctttt gtaaatggag agagtttata ctatgcaaat ataaatagaa
     6541 ataaaaaagg tatcactcta aaccttaaat cagaagaagg aaaaaatata tttttagaaa
     6601 tggtaaaaca tgctgatgtt gtagtagaaa attacagacc aggtgtaatg gaaaagttag
     6661 gtctgggtta tgatgagttg gctaaagtta acaatcaaat aatttatgga gctgtatcag
     6721 gatttggatg ttatggacca tattcagaac gtccaggtta tgatattatt gcacaggcaa
     6781 tgggtggtct tatgagtata acagggcaag aaggaggaga accaacgaga gttggtaatg
     6841 ctatgggtga tgtactaggt ggaatgaatt taactattgg tatactcaca gcactcaacg
     6901 ctagaaaaat aataggaaaa ggacaaagag tagatgtttc cctcgttgat tctgttgtat
     6961 cgtctctcga aactggtata cagagatact ttgattcagg acaacctgaa cttatgggta
     7021 atagatatgc ttcagcctat ccttatgact cttttaaggc aagtgatgga agttttgtaa
     7081 taggctgtgg taatgacaaa ctttttgaaa agttatgtaa aaaagttctt tttagagaag
     7141 accttttaat agatgagaga tttaaaacaa atcttaagcg ttgtgaacat tacaaggctc
     7201 ttaaaccaga aatagaaaaa tggtctgtta agcatacaat aaaagaagca gttgacttaa
     7261 tatcagaggc tggagttcct gctgctccta tctataatct tgattgtgtt acaacagatg
     7321 aacatatagc aaaagcaaga gaaatgattg tagatattag tcatcctatt ataggaaata
     7381 tgaaagtcaa tggaaatcca gttaagttga tgacaacaaa agctaaaatt gatatgccag
     7441 caccatctct aggggaaagt aacgaggaga tttataatgg ttggcttggt tttgatagtg
     7501 ataaaataaa agatttaaaa gaaaatggag ttatttgatt aaaaaatagc taaacacact
     7561 tgtgacttta gtcatgggag gttcagttag aataatatca aaaaatatta aatcaatact
     7621 gtaaatcaaa tatagaattg tcagaagcat ttaagatggg aaaagctact tagacattga
     7681 gtctttgtgg cttttcctat tttcattaag aactttatat tatcagaata gatatgtact
     7741 aaactaaagt attaacaatt taaattaata gaaaataaac tacctaatta tgttgaataa
     7801 agactacttt agtgaaaata aacttataga gatgctattt aaattatttt aatggaaata
     7861 tcaaaaatga gactgcatt
//
LOCUS       DACTGW010000177         7842 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.177, whole genome shotgun sequence.
ACCESSION   DACTGW010000177 DACTGW010000000
VERSION     DACTGW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.177"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(221..1039)
                     /locus_tag="I9573_002869"
     CDS             complement(221..1039)
                     /locus_tag="I9573_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT4797476.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNNTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDSVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            1207..1788
                     /locus_tag="I9573_002870"
     CDS             1207..1788
                     /locus_tag="I9573_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT4797477.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            1785..1979
                     /locus_tag="I9573_002871"
     CDS             1785..1979
                     /locus_tag="I9573_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797478.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2143..3885)
                     /locus_tag="I9573_002872"
     CDS             complement(2143..3885)
                     /locus_tag="I9573_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAT4797479.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEDLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            4314..5690
                     /locus_tag="I9573_002873"
     CDS             4314..5690
                     /locus_tag="I9573_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAT4797480.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            6001..6672
                     /locus_tag="I9573_002874"
     CDS             6001..6672
                     /locus_tag="I9573_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAT4797481.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            6863..7660
                     /locus_tag="I9573_002875"
     CDS             6863..7660
                     /locus_tag="I9573_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAT4797482.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         2902 a          947 c         1213 g         2780 t
ORIGIN      
        1 tatcctaatc aaattctagt cattcgaaca taacatcaca agtgatttat ctgacaaatt
       61 aagataatat cctaattaaa ttgtagatat ttatacataa cactaaaagt aatttgttta
      121 acaaattaag ataatatcct aattaaattg taggtattta tacataatac taaaagtaat
      181 ttgtttaaca aattaaaata ttatccaatt aatataaaaa ttacttcttt ttaactggga
      241 agcatacctc tgttacccaa ttatcaggat tactatcgtt gccaggactt acatgataaa
      301 tattaaacat aggaccatcc acatcatagt tattatctga tatccaatta ccaatagcct
      361 cacaagcgtc tgcaacttga ttgaaatttc cattaacaat tgcagttgca gcagtcacac
      421 ttggaacagt cttaaattta acatgttcag tatctttata ctttccagat acagcaactt
      481 gtacttcaac atctacagaa tcttctttat atccagtatc ataaaagact gctttagaat
      541 actttggggg ttcaatttga acattctggt cttttgtctc taaaaatgct tgatgccata
      601 acattccttc gtcttgataa gttggtataa cccttctaag tgtcatcata tattttggtg
      661 caaaattctt tatagttaca tcataatttt tcataatatc atttcctcca attcttttaa
      721 tagtggtttc tatttttaat agttttttct gtgtatcttc taattgttct tttacttgag
      781 aatggtggat atttagatat tttataagac tttctttatc gttatattct gtcaaaattt
      841 cttttataga atacaagcca aagcccatat cttttagtgc atggattcta tttgtagtag
      901 aaagttggtc tgcactatag tatctatatc cattagtgtt attcgtatga gaaggagtta
      961 aaagtcctat ttcatcataa tgacgtaaca tacgaatact aattttactt agctttgaaa
     1021 aatcgcctat cttaaacatg tttttgtctc ctttataaag gtacttttag cttaatatta
     1081 atataaacca tatcataatg ttagagtcaa ctaaaattat aattaaatta tattgacata
     1141 tgatgaaaaa aatattatac tgaacatgtt cagaacctaa aatgttaatt attttggagg
     1201 tctattatga aaagtaatga taaagatgct agagaacgta taattgaagt tacattgaat
     1261 cttttaaatg aagtagatga tattgaggag attacagtta gaaaaattgc tgagcgtgca
     1321 aatgttggag ttggtttaat aaattatcat tttaaaacaa aggataattt gttgagtact
     1381 gccataggag atgtgatgtc aaatataata gcagagctat atgacgatag tgtatatact
     1441 ctaagaccta ttgaagattt gaaaaattta ttaaaaaaat tgtgtgatac tggattacat
     1501 tatgaaaaag tgcttccttt tgtgttgaat caatgtataa ccaatggaga tatgcaagca
     1561 gaacttgata ttgtccctat gcttcgtaaa atttttggaa ataaaaaaga tgaaatgagt
     1621 ctaagaatca ttgccctgca aattatttta ccaatacaga tttctgcttt atctacagaa
     1681 tcatttcagt tatacagtgg tataaatata aagaataaat atgagcgaga taaattcatt
     1741 gatattttaa tagaaaatat tattggagag gatgttgatg taagatgaaa attataacta
     1801 ttggttcaag tcttatcact gttttacttt ttctttcaac tatggtttgt gggttctgga
     1861 ttaagaataa taaggtaaca gatgcaagtt ctattaagtt ccatatgaat agtgctattt
     1921 ttacagggat atttttattg atttccacaa tccttttaat tatatatata aaaaaataat
     1981 aatattatta ataaataaag tatcagaaat tataaagaca agagtcttaa aaatttctga
     2041 tactttacca cataatttta ttaaacatta attgtgtgtg aaaaaatatc acttatttca
     2101 atctaatact aaatcttaag agtgagtata aagtattaga aattaagaag cattttttag
     2161 ttcaagtgga ttatctatac caaaataatc atataaagta gagcttattt cctctaaatt
     2221 tccatcttgg aaaggtaatt gtaaatttac cttttctacc acatctttaa atccagcatc
     2281 agtattttga tttaaaaaat ccatatatac atcaacagct tttctgtaat cttcttgtga
     2341 taattgccat atctgaagtg atacatcaga tgcaagacca taactcaagt aataaaaagg
     2401 aacttggaag ttatgagata caagtatcca aaaaggagca tttttttctc taagtatatt
     2461 attggaaact ccatattctt cctctaaatc aaaaaacaat ttattgatat catttagact
     2521 catatatgga ttcttgtata taatttcttg aaattcatca taaagacaag catcaataat
     2581 agtatttaaa acaatcgata agtgttcttt ttttatagct tcagattgtt taccaaaaaa
     2641 atcatcaaaa tatttataaa atagaatttc taaagatgtt gaatgaactt cacatacatc
     2701 tatacttggt tgcatcttat tatttaagtt aatactgttg tagtaattat aaaaatgacc
     2761 aaactcatga gcaaaactag taacacctaa aaaagaattg tcccatgtat taaacaagaa
     2821 aggtgcttta tatttattta tataagtagt atatcctcca ggcgatttgt tttttccaga
     2881 agaataatcg tacaagtcat attttttcat atagtcgaag ctctctttta gttttggaga
     2941 tatatcttca agtactttat caaacttcct aaatgagcga tttttatata ctttaatata
     3001 aatagaagaa tcagatggtt tagatgaaat ctctctatat aatggaacta tgtaatcttt
     3061 tacatcttgt ctaaattttt tagcttcttc ttgagaatag tctttgttta ggttcatata
     3121 ggcataatct atataattct taaatccttg cttacttgct atttcagttc ttgtctgtat
     3181 aagttctaag aatatgttac caaatatagg attgtatttt tttaagtaat ctgaataaat
     3241 tttcacatat tcttcagggc taagatttgg tcttgacaag gcttcttcaa agtctacctc
     3301 tataccatct attgatactg tagatttagc taataagtct tcatatttta caactaggtc
     3361 ttgctctttt ttaagtaaat cttcaacttc tttagaatta agttttccaa tgttctttag
     3421 ataattaaat gtatcctttc caagttcttt ttctaattct ttagagaatt tagaagttac
     3481 aaatctatct tgaaagtctt tgtatgacat gtcaacatct actgatttac tcataagata
     3541 cttatattcc ttagagtaaa attcgtctga tgaatctatg ttatttctaa tttttgctat
     3601 agtaagtgta ctgtaaaaat cctgataaat tttatcaact tcattaaata aatttaattg
     3661 ttccttagct gttttagctg ttaaaagttt gtcgttacac ttgttaatgt tttcgcaaat
     3721 tgactgtata ttaggacgct tatattccat tttttcaaaa ggaatagttc ctctaacaga
     3781 atcttttttt gtatcattag tataatttct tatgtatgat gcaaatataa taaaaactgc
     3841 aattaaaatt agaaaaacaa atattttctt cttggattta accataaaaa tcctccaatc
     3901 atggatatag ttattaatta ttttactata ttaaatataa aaaatgcatg ttaagaaaat
     3961 ttaacataaa gatatagata agattataaa taatataaaa aatttaactt caaaaaagta
     4021 tatgaaattt atactggtaa aatgataaat aaattttaag tacaaaatag taaaagctat
     4081 gatagtttta gacccattat atgtaaaaaa tatagatgtt tttggtgcaa tttaaattaa
     4141 aagatatata taaaaattta aaatattcca aacagaaatt ataaaaatta ctatcattgt
     4201 ctttatgcaa tatttatact ataatagaat aataatttag aggaaagctc taaaaataaa
     4261 caaattaagg gtcttaacag tagtgatttt atgttaagga ggtataaaga aagttgaaaa
     4321 caatagttag aagacttatt ctctacgcag atggaatgcg tcctactcaa ataatggtat
     4381 ctgggtttgc tgcgattatt gtaatcggag cattgttgtt aacactcccg atagcgtcgc
     4441 agagtggaga gagtataggc ttattaaatg ctctatttac ggctacttca gcggtttgtg
     4501 ttactggtct agttatggta gatacagcaa cttattggag tttatttgga cagatagtta
     4561 taataacatt gatacaaatt ggtggtcttg gatttatgac cgtagccaca atgttttcac
     4621 ttatggcacg aaaaaaaata caactaagag aaagattatt aatacaagag tcattaaatc
     4681 aagctgattt atcgggactt gtaagactta caagatttgt attaataata accataacaa
     4741 tagagggaat aggtgcactg gttttatcaa ctgtctttat tccacaattt ggtttgtcta
     4801 aagggatatg gtatagtgta tttcacgcaa tatcagcgtt ttgtaatgcc ggatttgact
     4861 tgatgggcag tgtaagtggc ccatttacat cgttaaattc ttatgtaaac aactttacag
     4921 tatcaatgac tgtttgcgca cttatagtgc ttggagggct aggattccct gttgtacttg
     4981 atattgtaag aaaaagaaga ttttcaaaat taaatgtgca ttctaaggtt gtattgtttt
     5041 caactgctac acttattttt gtgggtgcat tatttatttt tcttatagaa tttaatcaaa
     5101 aagctactat ggcggacttg cctttaaagg gaaaagtact atcagcaata tttcaatcag
     5161 taacagcaag aactgctggt tttaatacac ttgatttagc tactttacga gaaagtagtg
     5221 tatttgtaat gataattttg atgtttattg gagcatcacc tgcatcaact ggtgggggta
     5281 taaaaacaac aacactagct gttttaatca ttacagttag aagttttcta tcaggaaaat
     5341 cagatataga agcttttgaa agaagattag ctccatcaac tataaaaaaa tctcttggta
     5401 tatttgtaat cagtatatca gcagttattt ttggaacatt gattatttca ataactcagc
     5461 ctaattttac attagttcaa tcagcttttg aggttacatc agcacttgct acagttggtt
     5521 caagtttagc tggaactcca aatttaaatg ctttaggtaa gattataata ataatcttta
     5581 tgtttatggg tagagttggt tctcttactt tatttatggc tatactttct ggtggtagaa
     5641 gaaagagtca accaatcaga tatgcagaag gtaaaattat ggttggttaa gttaccaagg
     5701 agtaatataa atttataatg gatttagagt agcactgtaa atctgttgat aaaataaatg
     5761 agcctagatg taattatatg aagaaataaa atcaactaat aaacaaaatt aattaatgaa
     5821 ataaaaaaac agggaaataa aatacaaata aaaaggtaaa tcaaaattaa attaatctgt
     5881 gtatataaaa ttatatattg tgtataagtt ttgtatgtgt aaagtatata agttttgtat
     5941 gtgtaaacaa ttaatttaag agatattata taaattgtgt attagggagg aagtttcgag
     6001 atgaagcaat atatagtcat tgggtgtggg agatttggaa gttcagttgc gtctactatg
     6061 catcttttag gacatcaagt aatggcaata gacaaaaatg aagattcagt tcaaagtata
     6121 tctgacaagg taacccattc acttatagtg gatgttactg atgagcaagc gttaaggtca
     6181 ttaggtttag gtaactttga tgtagcagta gttgcaatag gttctgatat aagggcatct
     6241 ataatggcga ctcttatagc caaagaaatg ggtgtagagt tgataatatg taaggcaaag
     6301 gatgaattac aagctaaagt gctttataaa attggcgcag atagagttgt atttccagaa
     6361 agagatatgg gagtaagagt tgcacacaat ttagtttcgg ataatatatt agaccatatt
     6421 gaacttgacc cagagtattc aattgttgaa atcgtaactc caaatagttg ggttggcaag
     6481 acacttatag agcttgaatt aagagctaga tatgagataa ctgtacttgc tataaaaaca
     6541 ggtaaaaata taaatgttac accttctcca gatgaggaac ttacagccgg aagtatccta
     6601 gttataatcg gtcaaaatac tagtataaca gcgataacat ctggaaataa ggggataatt
     6661 agaagaagat aatttactat ttaatatata tttaattgca atgaaagtaa agagtatcat
     6721 ataattatga atagttatat gatactattt ttatttaatc gaaggtagta tttatttgta
     6781 agattagata aagagaagtt aaattaaaag taaggaggct gtgctaatat aaaaatttat
     6841 aagttattag catttagata aaatgattac aaatataaac agtaaggata atgaaaagtt
     6901 aaagtataca agagcactat taaaatcaaa gaataggaat aaagagtcaa agttcataat
     6961 agaaggatac agaatagtaa tgcttgcact tgaatgtatg gcaaaccttg attatgtatt
     7021 tatcaatgaa gaatttgaaa ataagaaaga acatgtaaaa ctattagaag atttggataa
     7081 aaaaaacaca aagatataca agactactaa taaaaacttt aaagaattag tggatacaga
     7141 aaatactcaa ggaataatag gtgtagtttc atttaagaaa aaaaaattaa gtgaaagtat
     7201 aaataaaaaa gataaatttg tattgatttt agatagaata caagacccag gaaatatggg
     7261 gactataata aggactgctg attctgctgg agtagatgcc ataatagcac taaagggatg
     7321 tgtcgatata tacaacccaa aagtaattag gtctactatg ggttctattt ttgatatgaa
     7381 tataattgat gcttcacaag atgaaactgt ggacatgctt aaatcattgg attttaatat
     7441 agtttcaagt tacttaaata cagaaaattt ttatgacaaa atagattatg gttcaaaagt
     7501 agcattggtg ataggaaacg aagcaaatgg aataaatgaa gaacttgtat caaagtctga
     7561 tattttggtt aagataccta tatatggtaa agccgagtcg ttaaatgctg cgataagctc
     7621 tgctatactg atgtatgaaa taaaaaaata cttaatttaa tgtattgtaa taaatataat
     7681 gtcatgttat aatcttgaat taaatagtag agtatcaaaa atagtcaata tatattaaaa
     7741 aaataattaa attaattata tattagatgt atatcatata aaaaatgatt gagttgatta
     7801 tgtattagat atatattata taaaaaataa ttgagttaat ta
//
LOCUS       DACTGW010000178         7832 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.178, whole genome shotgun sequence.
ACCESSION   DACTGW010000178 DACTGW010000000
VERSION     DACTGW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.178"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(132..731)
                     /locus_tag="I9573_002876"
     CDS             complement(132..731)
                     /locus_tag="I9573_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797483.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(932..1144)
                     /locus_tag="I9573_002877"
     CDS             complement(932..1144)
                     /locus_tag="I9573_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAT4797484.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDISIEKLKEVIAELGFELKGIE"
     gene            complement(1217..2932)
                     /locus_tag="I9573_002878"
     CDS             complement(1217..2932)
                     /locus_tag="I9573_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT4797485.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEEARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANINSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEKKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(2932..3486)
                     /locus_tag="I9573_002879"
     CDS             complement(2932..3486)
                     /locus_tag="I9573_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAT4797486.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(3479..4081)
                     /locus_tag="I9573_002880"
     CDS             complement(3479..4081)
                     /locus_tag="I9573_002880"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAT4797487.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNSLTHPRIREKIISEIEELR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSKQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(4090..6738)
                     /gene="polA"
                     /locus_tag="I9573_002881"
     CDS             complement(4090..6738)
                     /gene="polA"
                     /locus_tag="I9573_002881"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAT4797488.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAENNGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPAQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMVFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDTEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETIPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            7057..7139
                     /locus_tag="I9573_002882"
     tRNA            7057..7139
                     /locus_tag="I9573_002882"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7091..7093,aa:Leu,seq:caa)
BASE COUNT         2397 a         1338 c          786 g         3311 t
ORIGIN      
        1 caattaccaa atggtggtaa attatttaaa agaatatgtc aaaaaaagaa taagctcttt
       61 caaatgcaca aaaaatttat acccaagtta tacttaatca tgcaaattgg cagaacttat
      121 tctttaaaac tctaactttt aagtttttct ttgataatat aatttattac tttttttcta
      181 atatcttcag cattataatc atcttctact tctatatcca aaagttttgc tattgacctc
      241 atatcttctc tagtctttga cctatagctt gatattaatt catcttttat tttctctggt
      301 tctaaaagat attcttcatc tcctcttttg aaaacaacgt atttttgagc atatgaactt
      361 ctattatagt atatatatct tgatattttt cttaaaattt gattatatga aagtcgcttg
      421 ttaatatcaa gttttaagta ctctgaaaaa gcaaatagct ctttttttga tttaaataat
      481 gcttctttct ttatagtcct tgatattgaa gttacacttt cttgatatgc acactttttt
      541 aacattttta aatatactct attaagacca atactttgat tgaatttttt tatattatta
      601 ttaataatct tatccaagtt accttcaaag tatttttttg ctcctttttg tgatacataa
      661 ctctcaagtt ctttgtgggc catatcaagt atcatttttt ttaaatcctc tttatatccc
      721 ctagattcca tgatactccc tcccttagct atttatagta tattcaaaaa gctcaagaaa
      781 tgtgagtcat tacaaaattt ttttactttt tatgtatata ttaatttaaa aggggagata
      841 atagtaatgt aaaatcaaac atactcaatc tcccccattt ttagacctct attactcccc
      901 caatagaggc ctttttttag tttatcaatt tctattcaat tccctttagt tcaaatccta
      961 attctgctat cacttctttt aatttttcaa tagatatatc atctttcata tctacagatg
     1021 cacacttatt ttctaaatcc acatctaaaa cttcaatacc atctatatct tctgttaatg
     1081 cagttttaag atggtttaca caatgaccac aactcatacc ttctactaat agtttcttct
     1141 tcatttaatt acctccttat accccataag ggtattctat catatagttt atacttttta
     1201 aatagaaatt aatcatttaa tttgattcat tcgattttat atctaaatta gatattccct
     1261 catatacatc aaaatcattt ggagtaattt taccttttaa tctactatta cttacaatat
     1321 agttgtttct cacagctaaa cttataaatg ttgcattatc ttttatatat gtctgtacat
     1381 ctttgtatgc tttttttgtt tcaatttcag acgtagaact tgtaagtgca gacatatatc
     1441 catttagctt atcatcagta tatccactac ttgctataat actacttgca tctggtataa
     1501 ttgatagttt ccatccaact aatgctaaat cataattttt agaattaaga gcattttcca
     1561 tatccttatc tgacagctga tttactgtac tatttattcc tatagctttt aagttttcat
     1621 ttattatgtt tgcagttttt attctctcac tattatctct gtttactatt atctttaagt
     1681 tcatttcaga aagacttttc tttacttcct ctttttctct attttttttc ttttcagctt
     1741 cttttttgat tctatcttct tctgcttttt tagcttttgc ttcttcttct ggcgtaagct
     1801 tctttgagtt ctttttagct gattttttct gattttttac tttattgtca ttttgattta
     1861 ctgagtttat atttgcatat tctaccttag caagacactc tatcgcctta tctttattaa
     1921 actctaaatc cttcatttca ctattatagt attttgattt tgaattaagt ggaaaattaa
     1981 ctggagtagc atcatccata taaccttcac taataattct atctttatct atagagtgag
     2041 caattgcttt tctaaaatta acatctttaa aaaatggatt attataatta aataatacac
     2101 actcataatc tcttccttga tattttgtta tattaaactc tttttcttga aacttgctta
     2161 aatcatttaa tgtaacattg gttatatcac tatcaagagc cataaccata gatgtccttg
     2221 cctcttcatt aggcaccata cctactttta tgttcttcat ggtctttgga acttcttcat
     2281 agtaatcttt gtttacagat aaaaccattc cttctctttc tgtatatgat tctattttat
     2341 atttcccatt tcctatcata ttattattct tgtcatttac atcctttgaa tctaaatgat
     2401 tttgagatac gattggaaaa attaaactat caattgaaaa agcatatcta gctttaaatt
     2461 ttattgtaaa atctttatct gatttaatat tcacagaagc tatattttta gtaaattcat
     2521 tatatggact atctgcactt ttttgaatta aaccaatagt atagtttaca tcatttgaag
     2581 taacattttt tccatcatgc cactttgcat cttttagctt tatatctata ctcattccat
     2641 cctgagctat attatactcc ttaactaatt gaggaattac attgtagttc tcatctatag
     2701 taaataaact atcataaact aaattcatta tataaccaac agacttatca gtattagtta
     2761 taggatttat agtttttgga gttaccattg ttaagtttat atacttagca tctatagaac
     2821 tttgactttg taaacttgaa tcactactac tttgttttgt tttagtatta ctacaaccag
     2881 ctaccataag agttatagct aaaacgactg ttaagacttt tattctcttc attagttttt
     2941 tcctttcttg ccatatatct tattataatg ttcgtaatta ttttttactt tttctacata
     3001 ttttgaagtt tgcttaaaag gtatatcatg tagattttcg ccatctttac tatattcatt
     3061 attttctaac cattttttca cattacctga accaccatta tatgcagcta tcactaaatc
     3121 taatttacca aattctttgt ataacttact taaataccaa cagccaagtc ttatattagt
     3181 ctctgggtcg aaaatatcca cattttttaa atttagttcc tctgctcccc aatctctagt
     3241 tatatctaat atttgcatta atccttttgc ttcctttttt gaaacagcag aactattaaa
     3301 cttactttcg gctttaataa cactgtaaac tatattttca tctaaattaa attcttttga
     3361 atacttttct acatactctg aatatttttt aggatataaa aatttatgta ttactttgct
     3421 ttccattaat agtgccccaa ataagattat aaaaatagat agaatcaata ctttcttact
     3481 attcacgcca attctccttc atgtactcaa taaatttatg tgctttactt tctaattcag
     3541 ttattgttcc cgaattatct attatataat caccatattt ggatttttct tcctgactca
     3601 tctgtgaatt tatcctacta agtgcttcct gcttactaca attatctctc ttttgtattc
     3661 tactaatttg tacttcttgt ttgcaagtaa ctactaatag cttgtcaacc atatctaaaa
     3721 aaccactttc taccaatatt gctgcatcta atactactat attttcgcct ttttttctca
     3781 attcttctat ttcacttatt attttttctc ttattcgcgg atgagtcaag ctatttaatt
     3841 ccttaagttt ttcttcatcg ctaaaaacta ttttcccaag agcttttcta tttagagttc
     3901 catcatcatt ttttatactt tgcccaaaat ggacaaatac tttttccaat aacaacttat
     3961 cctcaaatat cttcctggat ataatatcag catccactat agaaatatta agatttctaa
     4021 atatatttga taggctgctt tttccacagc ctattcctcc tgtaagtcct aatatcaaca
     4081 taaaatcacc tactttgtct cataccaaga actacctaca tttaaatcta catctaagtg
     4141 aacatccata tttacagcat tttccatttc ttcttttact attttcttaa ctatatctat
     4201 ttcatcatca attgcttcaa ctatcaactc gtcgtgaact tgtagtataa gttttgattt
     4261 caaattgttc tcttccaatt ttttatgaac atttatcata gcaattttta ttatatctgc
     4321 tgcacttcct tgtattggtg cattcattgc aaatctcttt cctctatttc tttccataaa
     4381 attgcttgac tttatttctg gtatatatct tcttctgttt aaaattgtta cagcatatcc
     4441 atcctcacta gccttttcta ctgttgtgtc cataaactct tttattttat ggtatttagc
     4501 aaaataactt tcgatatatt ctttcgcctt tggaacaggt atatttaaat catcagctag
     4561 accaaagtca cttttaccat atataatacc aaagtttaca gctttagctg cacctctcaa
     4621 ttcactagta acatcttcca taggaacatt aaaaacttgt gaagctgttt tggtatgaat
     4681 atcttcatga tgtttaaatg cttctatcat attttcatcc tgactcatat gtgcaagaac
     4741 tcttagctct acttgtgagt aatcggcatc aaccaactta cagttatcat ctgcaataaa
     4801 cacttttctt atttttctac ccatttccat ttttataggt atattttgaa gatttggttc
     4861 tgtagatgaa attcttcctg tagtcgttat agtttgatta aatgatgaat gtattctatt
     4921 actttctgga tttattatat ttaaaagtcc ttcaacgtaa gtagaattta actttactat
     4981 ttgtctatac tcagttatct tatctattat tggatgctta tctcttaatt tttctaaaac
     5041 atctgcatta gttgaatatc cagttttagt ttttttaatt attggaagct ctaatttttc
     5101 aaataatata acacccaatt gttttggtga atttatattg aatggctctc ctgataattc
     5161 ataaatttcc ttttctagtg tagctattat ttctttaaac tctacactca attcatccaa
     5221 tatacttttg tctactttta taccttcata ctccatatga cctaagactt ctactaaagg
     5281 catctctaca tgataaaaaa gaccatccat actcatatcc attagacttc tttccatcat
     5341 aggaattgtt tctttaactg tgcatattat ttgacccata tactctgcta aatcctcaaa
     5401 ttctaaatcc tcatagtttt tagatttaac accctttcct aataattctt cttttgattt
     5461 aacctttttt gataaatact tcatagctat tgcactacaa tcatatgagg tagatgaagt
     5521 tgaatctatt aagtactcag ctattgttat atcaaaatac atattttcta agtttatatt
     5581 atatggtttt aaagatatat aatcatcttt taaattatat ccatactttt taatttcatt
     5641 atttgaaaat atactatcta actttgacac ttcatttgaa tctacataat atattttttc
     5701 tccatctaca cttataaaaa tatatattat atttttttca agtatattac cttgtcctct
     5761 aacagttttt aaaataactt gtcctttttt gtttacttca tctataaatt tttctgtatc
     5821 ttttaatttt aatatttcta ttttttcttc tacgtgttct gtgtcatctg aatcgaccaa
     5881 atcaactaat cttgataaga gacttgtaaa tccaaagtat ctaaatttat ctaaaagttc
     5941 atctttatca taatcgccaa aaaccatact atctaaatcc acttctattg gcacatctct
     6001 tataattgtt gcaagtcttt tactttgaat agctatttct ttattttcct ctattttctt
     6061 ttttacacta cctttaagct tatctgtatt ctctattaaa ttctctatac tagaaaactc
     6121 ttttataagc tttatacctg ttttttcacc gattccaggt actccaggta tattatctga
     6181 cttatctccc ataagacctt ttaaatcaat aaattgtgct ggagtcatct cgtatttttc
     6241 aattacagaa ttaaagttgt attcttcaac ttctccaact cctttttttg ttataagagt
     6301 agttgtatta tctgaagcaa gttgaatagc atctttgtct ccagttacta tgtatacctt
     6361 atacccatta ttttctgctt ttttagaaac agttcctata atgtcgtctg cctcataccc
     6421 atctatttcc aatctattta ttttgaattt atctaaaagg tcttttaatg gctgtaattg
     6481 ttctgccagt tcatctggca ttccctttct tccagcctta tactctttat attctaaatg
     6541 tctaaatgta ggtgcttttc tgtcaaaggc tacacttata tgtgttggtt tatatatatc
     6601 tatcatttta aatagcattg ttgtaaatcc atatattgca ttggtcttta acccttcttt
     6661 attattcatt tctggcagag cataaaaggc tctgtttatt attgaatttc catctattat
     6721 aattaatttt ttttccaaat tatatcactc ctaaaaatta tctatttttg taacatatat
     6781 attatatacc aatatagtgt tatttaatat tcccataata ataatagtat attaaaatta
     6841 aatatgttgc aaacaatata tttttttgct ataataatat acatgacatg cccgaatggt
     6901 tgaattggta aatacaatgg attcaaaatc aactgtgttg attcataagt ttttataatg
     6961 ttttacagtt tgatgagtat tttgtattca atattcctct tgcatattct tggaaaatct
     7021 ggtaggatat acaagtagtt tggatttaaa atataagccg ctgtggcgga actggcatac
     7081 gcatacgact caaaatcgta cgggaaacca tatgggttcg agtcccatca gcggcaccaa
     7141 taaaaaaaac tctttagtat atactaaaga gtttttttat tgtaatattc tttcatcttt
     7201 atttctagcc ttgtataagt ataaatacca tagtcataac ttcttgaact cctaaaatat
     7261 atctaaacaa agtaggattt tttatgtatt cacttaataa atccatactt cttatcatat
     7321 tgattttttc ccaatctcta aaaattttga tacaaataaa aaatacagag aactacttga
     7381 tatactagaa ggttattctg taccatttgg tattgctgta ggtgttccag atggaaattt
     7441 accaaatgct agagatgtag tttataaagt ttctagagtt taggataaag tgttgcaaca
     7501 atcaattagt tacttgaaga gttaaatcag taatatacaa ataaattaat ggaagaggaa
     7561 gctatttaaa aagtagttta gatttttcat tgtaaaataa ttaaataaag aataaggtga
     7621 taagtatttt atttattatc ttattctttt ttatttgtgg aatgataaaa ataagaatta
     7681 agtgtaatac aatgtaaata aaatgtgtta attttaagta tattttgtaa agtttataaa
     7741 atctgaaaag ctcaagatat attttcacta taatggataa aatatcaaaa tattgaaata
     7801 aacaaaaaat aatgatataa taagaaaaaa ta
//
LOCUS       DACTGW010000179         7778 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.179, whole genome shotgun sequence.
ACCESSION   DACTGW010000179 DACTGW010000000
VERSION     DACTGW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.179"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(187..942)
                     /locus_tag="I9573_002883"
     CDS             complement(187..942)
                     /locus_tag="I9573_002883"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAT4797489.1"
                     /translation="MLKVIINGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1383..2372
                     /locus_tag="I9573_002884"
     CDS             1383..2372
                     /locus_tag="I9573_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAT4797490.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMIVLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVINATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2643..3359)
                     /gene="dapD"
                     /locus_tag="I9573_002885"
     CDS             complement(2643..3359)
                     /gene="dapD"
                     /locus_tag="I9573_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAT4797491.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLNEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3583..4332)
                     /locus_tag="I9573_002886"
     CDS             complement(3583..4332)
                     /locus_tag="I9573_002886"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAT4797492.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDNEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4399..5286)
                     /locus_tag="I9573_002887"
     CDS             complement(4399..5286)
                     /locus_tag="I9573_002887"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAT4797493.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5403..6404)
                     /locus_tag="I9573_002888"
     CDS             complement(5403..6404)
                     /locus_tag="I9573_002888"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAT4797494.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(6829..7710)
                     /locus_tag="I9573_002889"
     CDS             complement(6829..7710)
                     /locus_tag="I9573_002889"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAT4797495.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
BASE COUNT         2648 a         1278 c         1012 g         2840 t
ORIGIN      
        1 gactataaaa taataaacaa tataattcca tataccaata aataagataa tctttaagaa
       61 aaatgattat atttaaaagg taagcataaa atttataaac ttttagaata gaaatctcaa
      121 tcaattagat attcaaattg atgaatatga atacctaaat agattgagat ttattatgta
      181 caaaacttat cctagtatat ctgacatttt ataaagtcca gattgcttac caattaaaaa
      241 tttagctgca actatagaac cgtttgcaaa tacttcatca gattgagaat gatggcttaa
      301 tgtaagaact tcattatcac ctgcaaatat gacatcatgg tcgctgatta tattgccacc
      361 tcttatagag tgtattccaa tttcatttgg ttttctttta caatctctac catatctacc
      421 atgtgtattt tcactttcag gcaagacttc tctaattgca ttagctatca tagtagcagt
      481 tccactaggg gcgtcttctt ttctattgtg atgtttttct atgatttcaa tatcaaaacc
      541 ttctaacatc tttgaagcat ccttaactaa ttttattaat atattcactc caagagatgt
      601 atttgatgat aataatatag gaatgttttt tgatgcttct tctattttat ttaagtcatt
      661 ttcatcatat ccagtagtag ctaatactac aggtgtatta ttttttagag catatgacaa
      721 tattggctct aaagtctcat gatgtgaaaa gtctattatg acatcagttt tttcagttac
      781 atcagtcatt gttttgtaaa gctttgtatc actatgatga cctttttcat cagcagacct
      841 agctacacca caaaccaact ctaattcatt atcttttttt acacattctg ctaaaactct
      901 tcccattgtt ccattatagc cattaattat aactttcaat ataaattcct cctatgaaat
      961 taataaaaat ttgttatatt tctataagtt tattttatca ctaatattat ttatataaaa
     1021 taatattagt gataataagc ttacaattta tcttacatta aaaaaattta agtggatttt
     1081 tcaaaatata gataaacttc tttttcattg tataaatgat aaaaatatat ttgacttcat
     1141 atcaaaaaga gtgccccatg aactaaaaag ttaattttga gataccatat tcatatatca
     1201 tatgatttat ttggaaatca atattaatac attaaaataa ctaaatacat gtatgcataa
     1261 agatgtataa aaaatatata aacatatagt attaatttta aattcaacaa tttaattgtt
     1321 aaaagtgtta ttattaaatt agaaatatct taacttaata tactgagatg ggagtgatag
     1381 atatgttaaa agaagaaaaa acttatcaag agaaagacgc aaaagtattc aatggagtgg
     1441 cagctttgat aatgattgta ttatttttag tagtttcaat aggtatcatg gtttttggga
     1501 tagtaaaact tgatagtgga tttactggaa taggttcact tatgttaatt ttgggacttg
     1561 tatttatagt agtagatttt atcctatttt ttgggctaaa aatgattaat cctaaagaag
     1621 ctatagttct agttttgttt ggtaactatt atggaactat aaaaaaagaa gggtactatt
     1681 gggtgaatcc attttgttca gcaataaacc cagcagtatc aagaattaat gtatcgaaat
     1741 cttcatctga tgaaaaggta gatatatcaa ctaattcaag gggcaaaaaa gtatctttaa
     1801 aaacaatgac tcttaataat gaaaagcaga aagtcaatga tttattggga aatccaatta
     1861 taataggagt ggttgtaatt tggaaggtta taaatgcaac aaaggcagta tttaatgtgg
     1921 ataattataa tacattctta tctattcagt gtgattctac tattcgtaat gtttcaagac
     1981 tttatccata tgatgtatct gaagatggag atgaaaaatc tcttcgtggt agtagtcagg
     2041 aaatagcaga tagattaaaa gatgaattac aatcaagggt tgacattgca ggaattgaag
     2101 tgtgtgaagt gagaattact catctttctt atgcaccaga gattgcagca gcaatgttac
     2161 aacgtcaaca agcagaggct ataatagcag cacgtaaaaa gattgttgag ggggctgtaa
     2221 gtatggtaga gatggcattg actaatttaa gtgaaaataa tgttgtgact ttggacgaag
     2281 aacgtaaagc agctatggta agtaatctgt tagttgtgct ttgtggcaat aaagatgcac
     2341 aaccagtagt aaacagcagt tctatttatt aaattataaa aagtttaata gtttattaca
     2401 ttgatatatt gaaaaattga aggaaataaa aatacttgtc atcaagaaat aaaaatgata
     2461 taagaatgaa taagagaatt gtaaaaaaat aaaatgatta taaataaaag ataaaaatat
     2521 tataaaaaag agggtgcctc tgatgaatta aattcataca gagacacctt tttaattaat
     2581 tttttaaatt agtatattat tgtttaatta atctttctaa ttaataatat agtactattt
     2641 aattagtcta aatttctaag gtcttccata agcttagttt tgtcagccgt tttttcatct
     2701 ttcattttta taactttagc aggagaacca gcaacaacgg caccagcttc tacatcagta
     2761 gttacaacag caccagctgc aacaacagca cctttaccga ttctaacacc ttcaagaata
     2821 actgcattag ctcctattaa aacatcatct tcaactataa caggagtagc agaaggtggc
     2881 tctaaaacac cagcaacaac agcaccagca cctaagtgaa catttttacc aagagttcct
     2941 ctagctccaa taacagcatt catatctacc atagaacctt caccaataac agctcctata
     3001 tttataacag ctcccatcat tacaacagca tttttttcta tagtaaccat gtctcttatt
     3061 attgcacctg gctcaattct tgcatgttca tttaaagtat tttttagagg aatagcagaa
     3121 tttcttctat catattctac atgagtatct gttatatcat tcccatgttt atctaatact
     3181 tccattatat cctcatagtc acctacaaat atataagaac cattacttcc aaatacctta
     3241 aatttatcag ttgattggaa gtgtaaagag ttagtattta catatacctt aacaggtgtt
     3301 ttctttttaa cttcttttat ataccttgct atttcataag catcgtttaa atttaccatt
     3361 tgttaattct ccttgtattt cattatattt cttttattat atacttattt gtcaatttta
     3421 gtatatactt acttcttatt atttatattt tattttagtt agtttttatt gtatagacat
     3481 agtattaaat aaatttatta tatatgtttg atttgtattg cgtattatta tatctatatt
     3541 gcatatgttt aatttatatt gtgtattatt atatttatac tattatccca acatgctgtc
     3601 catattataa aatcctggtt tttgcttaac taagtatttg gcagcagcta tagaaccact
     3661 tgcaaagata ccacgagatt gagcttgatg gcttattgta agaacctcat tgtctccagc
     3721 aaatagagca tcatgttcgc caacaatatt tccacctctt atagcatgaa taccaacttc
     3781 atttggttgt ctttttgcac tacgaccata tctaccataa ttattttcaa cgtttggcaa
     3841 aacctctttt atagcgttag caatcataac agctgtgcca cttggggcat ctacttttct
     3901 gttgtgatgt ttttctatta tttctatatc aaatccttct agtaatttag ctgcatcttt
     3961 aaccaatttt actaatacat ttacaccaag agacatatta tatgattgga atacaggaat
     4021 aactttagcc gcttcacgta ttttgttcat ttcgttatca ttatatccag tagtagcaat
     4081 tactaaaggt gttttagtct ttaaaacgta agataagata ttatctaaat tagagtgatg
     4141 agaaaaatct ataactacat cagcttcctc aactatagta ctcatatctt cataaatttt
     4201 aaggtcgcct tcatagctat ccagacctct agctgcaaca caaaccaatt ctaattcatt
     4261 atcttctctt atagtatcgg ctaaaatctt acccattttt ccatcataac cagttgctat
     4321 aacttttaac ataatttcct cctatgatta agggctaact tttgaccaaa cttacttttt
     4381 accatgcatt aatctttact atgcattaac ttttagtcca aagttaacta attctttctt
     4441 taacacttct aaatttgctg ggtccatttc tgctagaggt aatcttaaat ctccaacatt
     4501 gaatcccaat aaattcattg cagtcttaac tggtatagga tttacttcta taaataaagc
     4561 tgcgattaga gcatccatac caagttgtaa ttttctagaa ccttcaatat cgccttcaaa
     4621 gaatttagct accaaatcgt gagtttcttg agggcaaaca ttagcaagta ctgatataac
     4681 tccttgacct cctaaagata ataaaggaag tatagtatca tcattaccag agtagatagc
     4741 aaagtcatct ggaactaaac tagctatttc tgcaacttgt cctaggtctc cactagcttc
     4801 ttttacagca actatgttat caattttagc aagttcaact ataaggctag gacttatatt
     4861 tacacaagtt cttccaggga cattgtaaag tataattgga agtttaacac tgttagctat
     4921 tgtttcaaaa tgacgcttaa gtccagcttt attagcttta ttgtaataag gtgttattat
     4981 taatagagca tctactccta atttttcagc ttcttgactt aagttaactg agtgcatagt
     5041 atcattagaa ccacttccag ctataacagg aactctttta ttaacttttt caacaacata
     5101 cttaatagta gcaagttgtt cttcatcagt catagtatta gcttcaccag tagtaccaca
     5161 agatactata gcatcagtac cattagctat ttggtattca actaattcac ctaatttgtc
     5221 aaaatcaact ttgttatcct ttgtaaatgg tgtaactaga gcaacagcag aacctttaaa
     5281 tatcatataa aatcaatccc ctctttttta tttttgtgtt ttatattaca gttacttcta
     5341 atgcttgttt ttgagttact attaatattt tacaaaatta tatatttata caagtttgta
     5401 tattatacta aatcgtatga taaagccatt tcagctattt gaattgcgtt agttgcagca
     5461 ccttttctta tattgtcagc aacaacccaa agatttacac cattatcaac actaaaatct
     5521 cttcttattc ttccaacaaa tacttcatct ctatcatcaa attctacagc agttggatat
     5581 tcgtttttag caggattatc aactactaca acacctggag cttcttcaag tgtttttact
     5641 aaatcatcta actcaaatgg attgttaaac tcaacattta tagattcact atgaccattt
     5701 tttactggaa ctcttacagt agttgcagtt atttttaaat catagttatc aagtattttc
     5761 atagtttcat ttaccatttt catttcttct ttagtgtatc cattttcagt gaatgaatct
     5821 atatgaggaa gacagttgta agagatttga tgagggtaga atccagtagg attaccgttc
     5881 attcctttct ctaaatcttc aacaccttta acaccagaac cagatacagc ttggtaagta
     5941 gagtaaacaa ctctcttaat tttgtattta tcatcaagag ctttaagggg aaccatagct
     6001 tgtatagtag agcagttagg attagctatt atacccttat gattttttat ggcttctgga
     6061 ttaacctcag gaactactaa aggcacatct ggattcattc tccattgact agagttatct
     6121 actactaaga ttccatttga agcagctatt ggagcatact tttcactaat acttccacca
     6181 gcagagaata atgctatttg tatgcctcta tcagtaaatg aagtttcctt taattcttct
     6241 actatatatt ctttaccaca aaacattact ttagaaccag ctgatttaga agaagagaat
     6301 aaaaatagat tttctattgg aaaatttctt tcttctaata cttttaaaaa tgttctacct
     6361 accattccag tagcaccaac tattgctaca tttacttttt tcatataaat tcaccccaat
     6421 tttaaattat taaaacatta ttttatttta gttatacaag taagatatgt taatattttt
     6481 atatatccaa aaactttaat attatattaa caactttaat tatatacaaa aaacgtacaa
     6541 aggtatactt tgtacgtcta atttatcact caaagtaaac cctgtagctc atcacctaca
     6601 aaaataagtg acagtattac acatgttatg tgcaatccca gaaaatatta ttgcaagaaa
     6661 tattttcttc ggctgtaaat cctttttcca atcattaaca cctgccagct actattggat
     6721 actcttagtt acagctacct ctaccctagg ttaataggat attaaaattt gattattcta
     6781 ataataatac atatttaaga aaaaatcaac ttatttatat aaatattgct aaaacttaaa
     6841 tccaaaattt gtcaattctt gttttaacac attaagatta gtttcttcca tttcagctag
     6901 aggaagtctt aaatcaccaa cattaaaacc taaaatgttc atggcagttt taacaggaac
     6961 tggatttact tctataaata aagcagcaat taaagcatcc atatcaagtt gtaatttttt
     7021 tgagccttca atatcacctt caaagaattt agttactaaa tcatgtgttt ctttaggaca
     7081 tatgttagca agtacagaga taacaccact tccacctaat gataatagtg gtagtatagt
     7141 atcatcatta ccggagtata tagcaaagtc tttaggtact aatttagcta tttctgcaac
     7201 ttgagctaag tctccacttg cttccttcac agcaactata ttatctattt tagcaagttc
     7261 ggccacaaca gatggcttta tattcacttt agttcttcct ggtacgttgt ataaaataat
     7321 tggcaactta acgctatttg ctattgtttc aaagtgaagt ttaagacctt tctcattagt
     7381 cttattatag taaggagtta ttattaaaag accatccacc cctaattttt cagcttcttg
     7441 actcatatga acagacaaca tagtattgtt tgaaccagta cctgctataa caggtattct
     7501 cttattaact ttttcaactg tatattttat tacagcaaaa atttcagatt cactcatagt
     7561 agtcgcctca cctgttgttc cacaaacaac tagtgcatct gtgccattct caatgtgata
     7621 ttcgattaat tcacctaatt tttcaaagtt gacattatta tcctctgtaa atggggtaac
     7681 taaagcaaca gcagaacctt taaataacat ataatcaaat ctcctttttt atctaaattt
     7741 aattttttaa gtacaataat aattatagta caaattaa
//
LOCUS       DACTGW010000180         7765 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.180, whole genome shotgun sequence.
ACCESSION   DACTGW010000180 DACTGW010000000
VERSION     DACTGW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.180"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            183..1502
                     /locus_tag="I9573_002890"
     CDS             183..1502
                     /locus_tag="I9573_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAT4797496.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMSIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(1577..2005)
                     /locus_tag="I9573_002891"
     CDS             complement(1577..2005)
                     /locus_tag="I9573_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797497.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(2096..3472)
                     /gene="hydG"
                     /locus_tag="I9573_002892"
     CDS             complement(2096..3472)
                     /gene="hydG"
                     /locus_tag="I9573_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014094795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HAT4797498.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(3473..4558)
                     /gene="hydE"
                     /locus_tag="I9573_002893"
     CDS             complement(3473..4558)
                     /gene="hydE"
                     /locus_tag="I9573_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HAT4797499.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSPDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNKDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(4594..5802)
                     /gene="hydF"
                     /locus_tag="I9573_002894"
     CDS             complement(4594..5802)
                     /gene="hydF"
                     /locus_tag="I9573_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HAT4797500.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVNKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(5817..6068)
                     /locus_tag="I9573_002895"
     CDS             complement(5817..6068)
                     /locus_tag="I9573_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019279311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HAT4797501.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(6449..7489)
                     /locus_tag="I9573_002896"
     CDS             complement(6449..7489)
                     /locus_tag="I9573_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAT4797502.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         2661 a         1175 c         1072 g         2857 t
ORIGIN      
        1 ttgataaaaa taaaatatat tgtttctaaa taacaaatat tttgaaaatt ataaaaacat
       61 atattgacaa tttgtgtcaa aaatactaac attaagtata taaagaattt tatatgagaa
      121 tatctttata aatcgcaata attttagaac ataaatttat ttaaagaggg gatgatttgc
      181 caatgagcga gataactagt caaatgataa gtacagaaga aaaaaaatat gttgctaaaa
      241 ctcaaaagat accttattat ccagtagcat ttaaatcagg tgatggtgca atgctatatg
      301 attatgaagg taatgaatat gtagattttt tagcaagtgc aggctctgca aatgtaggtc
      361 atggtaataa ggaaatctca caagcagtta aagaacaaat ggatgatata acacaatata
      421 cacttgcata tttccatagt gatcctcctg taaaattagc tgagaagctt gtggaaatag
      481 cccctggaga taatgataaa aaagtactat acagtgcaac tggttctgct tgtatagatg
      541 ctgctataaa attagcaaga gggtatactg gaagaacaaa gataatatct atgtgtgaat
      601 cttatcatgg aagtacttat ggagctatat ctatatctgc tttaagtaca aatatgagaa
      661 gaaaaatggg tccattgtta ccagaagttt atcacttcca ttatcctgat aaaaatagaa
      721 cagctaaaga atgtttagat gagatagaat atgcttttgc acactatcta ccagcagaag
      781 aggtagctgc aatatttata gagccaattg caggagatgc aggaataata gtgcctccag
      841 tagagtgggt acaaggttta agtaaaatat gtaaagaaaa tggaatatta cttgtaagtg
      901 atgaaataca acaaggtatg ggaagaacag gtaagtggtt tggtatagaa aattttggtg
      961 ttgaagcaga ccttatagtt cttggaaaat ctgttggagg tggattacct ttaggagcag
     1021 ttgttggaag aacagaaata atgcaaagtc ttgatgcacc agcacattta tttactttag
     1081 ctggaaatac aacagtgtgt gtcgctgctt taaaatctat agagataatt gaaaaagaaa
     1141 acttacttca aaagagtata gaaatgggag attatattaa agcaggattt gaaaaactaa
     1201 aagaaaaata cgatataatt ggagaaataa gaggaatagg gttatctata ggagttgata
     1261 tagttaaagg aaaaggttct aatgaaaaac atccagatgc tacagctaag atatgttata
     1321 gatgtataca aactggatta ataatgatat tcttaggaca atctacttta agagtacaac
     1381 cacctctagt aataactaag gagcaagtag ataaagcaat gagcatcata gattctgcaa
     1441 tagatgatta tttaaatggt agaattggtg atgaagtata tgaagtaact caaggttggt
     1501 aaaataaata ttaaatgaaa aaaagagtta tctcaatttt agaaagtaat ttctgaaatg
     1561 agataactct tttaagttac ccacaaataa gaaaattgtt tgattcatta aagactatat
     1621 ttcggatgtc gtttttgtga gtatcagaga cagtattacg agaataatta cctttattcg
     1681 tactatctgt taatttagtt ataattaaga tagctaaaag aataagaatt actgtgtata
     1741 aagcaataac atatggtttt cttttatatt tgtatttgtt attgaaaaaa gaatggccat
     1801 aattattttt atttacttta gatgtatttg tattcttttt cttgttacct ttctttttat
     1861 catccttttt tctattttta gggtaaggaa tgactttgtg tgtattatca agtttgatat
     1921 ttttattttt ttgaatattg ttttccaggc tagccttttt ttctccaaat tgaatagttt
     1981 tattggtatt gttgttatct ttcaatgtta atctccctcc cttaaacttt ccctttagat
     2041 aatattacta ctaacctatg gaatatacaa taaataatat ataagtaata aaattttata
     2101 ggtataaatc tctttctcca cgctctaagg cagtcatatt gttagatact atgtttctta
     2161 tatcttctct ttctatctta cctatttctt ctgcgataag ttttttaccc ataattttaa
     2221 gttcttcatc tccataatcg attaaaaatt catttaatgt agtaagagca tttggcgtac
     2281 atacattatg gatttgtcga cttttagcta agctcatgaa tctatcgcca gttctacctt
     2341 tacgataaca agctgtacaa taactaggta tatatccatc agtaatcagt tctttaagca
     2401 cctcaacagg gcttctatgg tctccaactt cgaattgttc tgtattgtca ccatcttcat
     2461 atgctttata tcctccaaca cctgttagag aacctgcact aatttgtgat acaccatatt
     2521 ttagtaactc atttctcatt tcagcagttt ctctagtgga cattatgatt ccagtaaaag
     2581 gaacagccaa tctagttata gctactattt ttttgaacat atcgtcagaa actaagtgag
     2641 gaaaatcttc taagctcata ccttcagctt ttttcaatct agggaaagat atagtatgga
     2701 aacctacacc aaatttttcc tctaagtgtt cgttgtgcat cataagtcct agaacttcaa
     2761 atttagggtc agataaacca aataaaactc ctgcaccaac atcatcaaca ccagcttcca
     2821 tagctctatc aaaagctgtt aaatggtaat aatagtcatt ttttatagat tgaccatgca
     2881 tttttataaa tgttggtttg tgataagttt cttggaataa tatataagtt cctattcctt
     2941 tttcttttag taatttatac tcatctacac ttgtagctgc tatgttaacg tttacccttc
     3001 ttatatttcc gttttcatta tatgttgaat aaattgcatc taagcaatct aagatgtaat
     3061 ttatatcaca atttttaggg tcttcaccag cttctagagc aagtctttta tgacccattt
     3121 tttctaatat tttaacctct tcctttatct cttccatggt aagttttttt cttttaaatt
     3181 tgttgcattt actaaaaccg caataaacac attcatttac acagtagttt gatacataaa
     3241 gaggggcaaa taaaaccact ctattaccat atatttcatt ttttatttgc ccagctatac
     3301 taaataatct atctagttgt tttttatctt ctacttcaag aagtgtagct atatctttat
     3361 aactaagttt ttcccttcta tcagccttat caagtacctt ttcaatatca tcagaagttg
     3421 aattttttgc atcttctaat aaagaattaa taagttcatg gtttatgaac atttacaaac
     3481 gcctccattc aatattatca ccgcgagaat agtcaacttc aagtcctaat gacttgatgt
     3541 tttcttctaa actttggtga tattcacaag cttcttgacc agtaaaggct ttgttatcgt
     3601 aaagagaata ttgttttcta aattcttgag gagaaaggtt tggcataatt acattgcaac
     3661 cagctaaaag acctgcattt ctaccactag ggtttataga tgccaaggct gttgtagcgg
     3721 gtaataaaac ttttggaagt agaagtcttg taattgaaag cataagtaat gtcttctcta
     3781 aatctccatg tttataatcc ttaaaaattg tatcatggtg tggaataaaa ggtcctattc
     3841 caaccatatg aggatttaac tcttttaggt ataataaatc acgaacaagg tctttgttgt
     3901 tctgaaatgg tgattcaacc ataaatccag cacctacctg ataaccaatt tctttaaggt
     3961 tttttaggca ttcttttcta gttctaagct caatgtttgg cggatgtaat tttttataat
     4021 gactgtctgt agcagtttca tgtcttaaaa gatatctatc tgcaccacat aaaaagtatt
     4081 ttttatacga atcataactc ttttctccta gtgacagagt tatggcacaa tcctcatatt
     4141 tattttttat acttgaaaca atttcacata ttttatcatc agtaaaatat gcatcttcac
     4201 caccttgcaa aacgaatgtt ctgtatccta ttttataacc tatatcacaa cattcaagta
     4261 tttcatcagg tgaaagtctg tatcttatga tatttttatt agaagattgt attccacaat
     4321 agtagcaatt atttttacaa tagtttgtaa gctctataag tcctctcaag tagactctat
     4381 ttccataatg tttatttctc atgtcgctag cttgtttata aagatagttt cttaaatttt
     4441 gttcgaagtt atattcctca ttgtcgatat tttctattaa atatagtagt tcatcaaaac
     4501 ttaatgaatt aatgctgtaa agtttatcta taaagtattt aatcttattt ttagtcatat
     4561 tcaaatatgt aattattaag tatagttaaa tttttaataa ttgaaaagtt caagtgatct
     4621 ttccaaaata ccttttacat atgctattaa gataccatag tttacaatag gtacattgaa
     4681 agattttgcc ttttctattc tagataacat tccagctcta ttcatcatac aagctccaca
     4741 gtgaactaca agggcatatt tatttatatc ttcagtgaag gatactccag aactaaattc
     4801 aaaagtaata tttttgccag ttttctttct aatcatatta ggaattttta ctgttccaat
     4861 atcatcagtt tgtctatgat gggtacatcc ttcagccatc aagattttat caccatcttt
     4921 taaattttct aaagcataag ctccattaat caattcttta aggtctccct tttgtctagc
     4981 aaacaatata gaaaaagatg taagaggaat atcttttggt gtatctttat ttacttgtgg
     5041 aaatacttga gagtcagtaa taacaagttt tggttttttg cctaaattag atagagtttc
     5101 ttttaaacta tcttcttttg taactataga aattgcacca ttatccaaaa tatctcttat
     5161 aacttgctgt tgaggaagta taagtctacc ttttggagct gctttatcta ttggaacaac
     5221 taaaacaact aaatcatttg gttcaattaa atctgatacc aatttgaact cagtgctatc
     5281 cttaggcaaa acttttatta tttcattttt taaattttca attccaattt tatctgttga
     5341 tgagacagaa acaactggac atttaacttt atttttagtt aagtttaaaa tttctgattg
     5401 attcttaata gtatcaattt tgttgagaat tagaatatga gggatattct tatcattaaa
     5461 cttttcaatt agtgacaaat cttcttgtga tataccaatt tggcaatcca ctactagtaa
     5521 agctatatct gtcttttcta aaacatctag tgatttacct attctaagtt caccaagttc
     5581 accaacatca tcaagaccag cagtatctat aagaacacaa ggtcctatag gaaggatttc
     5641 cattggtcta aatactgggt ctgtagtagt tccagcaata tctgagacta ttgcagcact
     5701 ttgatttgtt atagcattta ttatgctaga tttacctgca tttcttttac caaagagacc
     5761 tatatgtact cttaccgatt gtggagtaga atttagactc atttgtactc accaccttaa
     5821 acttcttttt ttgatatact agtttttaca gatacatgag gtatgttgcc caatttacca
     5881 gtcaaactat taatagtatc catttcgcca acaactgcta tacatacgat tgagacacct
     5941 tcttcttcgc aaggaatgcc cattcttcct ttaataatac ctttaaaacc tgatacaacc
     6001 tcattgaatt taaattgaca ttcttttggc tcttccaaaa tagcacttat aactgcaacc
     6061 tttttcataa atatcctcct aactcagaaa atatgtctta aacaaatcca aaatcaaaaa
     6121 acttttcttt gtaaagaaaa gtttctacaa aagaaaagtt attaattaca caagtataat
     6181 gtgctatatt aaactatttc cttacagatt agataactct tttctggtag ggttagcttg
     6241 taaaaattta atttaacaac aagtaactga agaaaatttt tatacaattt atataaatta
     6301 taataaaaat taactaaaga aaattgaagt tacttatata atagtagtaa attgaaatta
     6361 agtctataga aaaaccaatt tttgtataaa aataaaaatt aaagttaatt atttgttaag
     6421 aattgacttt ggtaaaatat ataattaatt attctttaag catataattt ataagtaatt
     6481 ttagtgtatt ttcaatagct tctatatgtg ttctttcata atggtgacta ttgtttgttc
     6541 caggaccaac acatgctatt tgaacatctt ctccccaacg aatagcttct gaagcatctg
     6601 aaccatagaa agtaaataca tcgacagcgt agtctaaatt attgtcttct gcaacatcga
     6661 caagcttgtt tcttaaatta aaatcatata atgataaatt atcttttgca actatagtta
     6721 caccatgttc actagatgtt tgacttggtc cagtaggtgc tatatctatt gctacaaatt
     6781 cttttgtttt ttcaggtaaa catttagaaa caccatgtcc aatttcttca taattactta
     6841 tataaaagtt agtagtatgc ttaggcgtta aattgttttc tttaaaatat ctacatattt
     6901 caagaaccat tgctacagct gatttattat ctatatatct agattttata aaaccagatt
     6961 ctgtagcctc aaatctatgg tctaggcata caaaatcccc aacacttatt cctaaatcta
     7021 atacatcttg ctttgtaaat actttttcat caagtctaat aatcattgtt tcttcatttc
     7081 tttcagtggc agcttttgtt tgaccatata tatgagtaga agcatactta aatacaacac
     7141 ttcctctgat ttttttacct tttctagtta taacagtgca gttttctccc tctatagctg
     7201 cccaacatcc accaccaact ttatggtatt taagagtacc atcagatgat atttctttta
     7261 ctatagctcc aagtgtatcc atatgtgcag atattgttat ttgatttgaa tcatcttcac
     7321 caggaagagt agctataagt gcaccttttt ttgtaagaat agtgtcaaga cctaaatttt
     7381 caaaatcttt tttgatttct aaaactgcat tttcagtata tccagctggg cttggaatgt
     7441 ccaaatattt tttcattaaa tttatagtat tagttaaatt taaattcata ttgtttcctc
     7501 ctaaataaat atatttatac aaattataac ataaatacaa atgttctgaa aactaaaatt
     7561 aaaagaatag aatctaaaca tatttaaaaa aaaagtatat aaaataaatt tgatttatat
     7621 tatatagatt taaagtaaaa atagttttat ttaataatta ataaaaataa atacaaaaca
     7681 aaataaataa ataataaaat atttagttta attatcttta caaataaaaa ttttagtaat
     7741 aaaataattg ttgaaaaaga tttta
//
LOCUS       DACTGW010000181         7651 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.181, whole genome shotgun sequence.
ACCESSION   DACTGW010000181 DACTGW010000000
VERSION     DACTGW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.181"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            29..1087
                     /locus_tag="I9573_002897"
     CDS             29..1087
                     /locus_tag="I9573_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAT4797503.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCIFLENCDFNRTIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1191..2375
                     /locus_tag="I9573_002898"
     CDS             1191..2375
                     /locus_tag="I9573_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAT4797504.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADGKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNEKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGVIKYYTSSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2582..3295
                     /gene="rpsB"
                     /locus_tag="I9573_002899"
     CDS             2582..3295
                     /gene="rpsB"
                     /locus_tag="I9573_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAT4797505.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3374..4285
                     /locus_tag="I9573_002900"
     CDS             3374..4285
                     /locus_tag="I9573_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAT4797506.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4370..5074
                     /locus_tag="I9573_002901"
     CDS             4370..5074
                     /locus_tag="I9573_002901"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAT4797507.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5100..5657
                     /gene="frr"
                     /locus_tag="I9573_002902"
     CDS             5100..5657
                     /gene="frr"
                     /locus_tag="I9573_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAT4797508.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5660..5863
                     /locus_tag="I9573_002903"
     CDS             5660..5863
                     /locus_tag="I9573_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797509.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5892..6626
                     /locus_tag="I9573_002904"
     CDS             5892..6626
                     /locus_tag="I9573_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAT4797510.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6639..7418
                     /locus_tag="I9573_002905"
     CDS             6639..7418
                     /locus_tag="I9573_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAT4797511.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3144 a          785 c         1460 g         2262 t
ORIGIN      
        1 attgttttgt actggaggat atatatttat gaattgtaaa tggatatcta agatagatga
       61 gagaaaaaaa tgtcatagag aagcagattc ttctggatat tgtatctttc ataaagaaaa
      121 taagtctgat gaagagatac agttgatgat ggatactatt cataaagaag agataagtga
      181 atttaatgga tttgtttttg aaaatgaatt taatgcggaa gaaatattaa cttacaatta
      241 taaaatactg gatttttctg aatccatatt taaacaaaaa gcaaatttta aaaaatatat
      301 ttttaaaaag aatattattt ttaactatac agaatttaga gataaagttt tatttaatgg
      361 ttgtatattc ttagaaaact gtgattttaa tagaaccata tttagtaaaa actatataaa
      421 tgaccgtata tttgaaaaag tgaaatttaa aggacctgat ttggtagtta ataaagtaga
      481 aaattttcca cgaatggatg gaataatttt tagcatgtgt acaaaatttg tgttaaagaa
      541 tgttgaatat ggaaaaagtg agtatgagca tggcaagata aattatagaa tagctagaaa
      601 tcaagcaaca aaaattggag aatatgagat gataggattt tactattata aggaaagaat
      661 ttatagtagt aaaataatga aacgttctaa ttatcctact ttcagtgatt atttagttga
      721 aaaatttttt gaccaaatag cacgttacac aacaggttat ggagaaaagc catggaatat
      781 attattagtg attatagcaa taataagtgt ttttgcttta ttatacttat ttgtaggcat
      841 agaaagttca aatagcacac ttgtagcttt agatataaac aatataggtg attactcact
      901 aagtgagatt ttcaaaatgt atatggattt atggtatttt agcatggcta catttagtac
      961 agtaggatat ggagatatgg ttgctacaag tttgattggg aaagcattgg caggtataga
     1021 agtatttttt ggagttacaa taggagcaat ttgggcatct gtgattatta aaagaatgat
     1081 aaggtaatat ttgtgtaaat atggataggt attaattact agatataaat tggaccaaat
     1141 agggaaaatt taaatataaa tatacttaat aatccaagaa ggtgaaaaat ttgaaaaatg
     1201 cactaaaaaa aattatatca ttagccatga ttctagtttt tataatacca aattattcat
     1261 atgcagatgg taaatctgta gacaagtatt caaaaacatc tatacttata gaccaagaaa
     1321 caggtagagt tttatatagt aaagaacctg atatgaaggt accattagct agtttaagta
     1381 agatgatgac ttttttactt gcaatagaag ctattgaaaa taaagaagta aaagaaactg
     1441 ataaggtaaa aatagacaaa tctattgcat cagtaaaagg gtcaagttat aaacttaaag
     1501 atggtgaaga agtgccactt atagagctta tgagaggtct tatgattgta tcaggaaatg
     1561 atgcggcaat tgctatagca aaacatattt gtaaaacgaa agaagcattt gtagatagaa
     1621 tgaataaaaa agcaaaagaa ataggaatga gtaatacaca ttttctaaat ccaaatggtc
     1681 ttccaatata cgatttaagc aaccccaaaa aaccagcaaa agaaaatatc tctactgcaa
     1741 aagatatagc tattcttgga aaatacatgt ttgaccatta tgaaaaacaa gtaacagcta
     1801 taactgatat ggagacatat acaaattcag atagaagttt tgaaaaaagc aatacaaatg
     1861 cattattaag aattatacca gaagtagatg gtataaaaac tggatatact ggcaatgctg
     1921 gatattgctt atcattttct atggttgtaa ataaaaatga gaaaaatgag aaaaaacgta
     1981 gagtaatagg ggttgtatta ggtactaacc ataaaaataa aagaacctct gcttcattag
     2041 ctctactgaa atatggaaaa gaaaatttca atatgaaaaa agtaattgat aaagatagct
     2101 ttattggtaa gaaatatata aaaggaatga aagagttaga ggttacatta aaagcaaaag
     2161 atgaacttta tacaatatta aaagatgatg aaagtataaa atcagaagtt aagattaaaa
     2221 atatagagta ccctgttaaa aaaggtgata ctatgggtgt tataaaatat tatactagtt
     2281 caggtgactt acttggaagt gtagatatag taagtaataa tagtatagat aacgtatcat
     2341 taaaaacaaa attaaaaatg aaatttgcaa attaaattca aaaaatggta taaaatacac
     2401 aaaaaaatat aaaaatagaa aatatggaaa aaatattgtt atttcttcgc ctctgtgata
     2461 tactactaga ggtgaaaaaa taaaataaat cacacacagc attgggatcc ttaaaaggtg
     2521 cctattacat ggttttttag ggagaagatc aagctggagg tataaaacca ggaggtaaga
     2581 tatgtcagta atatcaatga aacaattatt agaagcaggt gttcactttg gtcaccaaac
     2641 aagaagatgg aaccctaaaa tggctaagta tatattcaca gagagaaatg gaatatatat
     2701 aatcgactta caaaaaacag ttaaaaaagt tgaagaagct tacaagttca ctaaagaagt
     2761 agctgaaaca ggaaagccaa tcttattcgt aggaactaaa aaacaagctc aagatgcgat
     2821 aaaagatgaa gctgaaagat gtggaatgta ctttgttaat gaaagatggt taggtggaat
     2881 gttaacaaac cacaaaacta taaaaacaag aataaataaa ttaagagaat tagaaaaaat
     2941 ggaagaagaa ggcgtattta atgttcttcc taaaaaagaa gttataaaat taagagctga
     3001 aaaagaaaaa ctagaaaaat acttaggtgg aataaaagac atgcctgaat taccaggtgc
     3061 aatgttcgta gttgacccaa gaaaagaaaa catagctatt caagaagctc atagattagg
     3121 tataccagta gttggtatag ttgatactaa ctgtgaccca gaacaattag actttgctat
     3181 accaggaaat gatgacgcta taagagccgt aaaattaata actggtgcta tggcaacagc
     3241 tgtaattgaa ggtagacaag gtgctgagga agaagtagct gaagaccaag aataataatt
     3301 tgacaattaa atggtaaggg tcgcccttac catttataat atggaataga ataagtagga
     3361 ggaaacttaa aaaatggcta atataactgc tcaaatggta aaagagttaa gagaaagtac
     3421 aggcgcagga atgatggact gcaagaaagc tttacaagaa gctgaaggaa acatggaaaa
     3481 agcagtagat ttattaagag aaaaaggatt atcaaaagct gctaaaaaag ctggtagagt
     3541 tgctgctgaa ggtctagttg caatagaaat gaatgatgat aatacagttg cttctatggt
     3601 agaagttaac tcagagacag atttcgttgc taaaaacgaa gacttcaaag tgttcgttaa
     3661 agatgctgct tgtatggcat tagctactga taaagaagat atagcttctt tattaggtga
     3721 aactcataaa gagggaataa ctttacaaga agttttaaat aatagagttg caaaaatagg
     3781 tgagaaatta gactttagaa gatttgctaa agttgtaact aatggacaag ttgctggata
     3841 cattcacggt ggtggaaaaa taggtgttct tgttgaaatg gaaacagaag ctagagatgc
     3901 taaagtttta gaattaggta aagatgtagc tatgcaagtt gctgctatga atcctaagta
     3961 tgtttcaaga gatgaagtag atgctgaata catagcacat gaaacagaag tattaactca
     4021 acaagcttta aatgaaggaa aaccagctaa tatagttgaa aaaatggtta aaggaagatt
     4081 agaaaaagaa ttaaaagagg tttgtttatt agagcaaact ttcgttaaaa atccagatat
     4141 aactgttaaa caattagttg ctgatgttgc taaggcagta ggttctgata taaaagttgt
     4201 taaagttgtg agattcgaag ttggtgaagg tatacaaaag agagaagaaa actttgcaga
     4261 agaagttgct aagcaactta agtaataacc aaagagagaa cacgcaggtg ttctcttttt
     4321 taaaaaatag aaaaaattgg atatgaagga ttgaaatagg aggtttctga tggataaacc
     4381 aatgtacaaa agggtattgt taaaattaag tggagaagca ttagcaggag aaagaggttt
     4441 tggtataaat aatgatgtag ttaatgatat tgctattgct ataaaaaaaa tacaagaaat
     4501 aggtgttgaa gtagctgtag tagtaggtgg aggaaacttc tggagaggca gaaccagtga
     4561 aggaatggat agaactactg ctgactatat aggaatgtta gctacagtta tgaatgcaat
     4621 ggcgctacag gatgcactag aaaatataga tgttgctaca agagttcaaa ctgctataga
     4681 catgagacaa attgcagaac catatataag aagaagagca gttagacact tagaaaagga
     4741 aagagttgtt atatttggtg caggaacagg aaacccttac tttacaacag atacaactgc
     4801 tgctttacgt gctgctgaga tggaagcaga agttatattg ctagctaaaa atgttgatgc
     4861 agtatatgac aaggatccaa aagtacatgc agatgctaag aaatttacag aattaagtta
     4921 catggaagtt atacagaaag aattaaaagt aatggattct acagctacat ctttatgtat
     4981 ggataataaa attccaataa aggtatttga gcttacaaca gagaatataa taagagcagt
     5041 aaaaggcgaa aatataggaa ctactgtaaa ataatgaaga atgaaggagg agtaaaatta
     5101 tgaaactgga aatacataag caattagaag agaaaatgaa tgggacaatt gatgctttaa
     5161 agtttgaatt tggaactata agagcaggaa gagctaatgc acaaatgcta gacaaaataa
     5221 gagttgatta ttatggaact ccaactccaa taaatcaaat aggtgctata tctgtaccag
     5281 aaccaagaat attaatgata tctccttggg ataaatctgc tatgcatgaa atagaaaaag
     5341 cgatagctaa ctcagactta ggattaaatc catcaaacga tggtgaggtt ataagacttt
     5401 ctgtacctgc tttaacagaa gaaagaagaa aagaattagc taaaaaagct agtaaagctg
     5461 cagaagaatt taaagttaga ataagaaatg aaagaagaga cgccaatgag aaaattaaaa
     5521 agatggaaaa aggcggagaa ttaactgaag atgaattaaa aaaagcacaa gatgaagttc
     5581 aaaaaatgac tgataaattt ataaaagaaa tagatacatt attatctaaa aaagaaaagg
     5641 acataatgga ggtataatat tgaaaaaata ttataacctt ttaggtcttc atgtagatga
     5701 ggtaaaacaa tattttgatg agcaaaatat aaattatacc gtaaaatcta ttgaaggtaa
     5761 aaaggataaa gaaaaacttg tagttcctag agttataaaa atatctgaga taggtgatag
     5821 tgtagaactg ataatcacat acttttctga ctccttagtt taaggagtca gaacaatatt
     5881 ggaggcaaaa tatgaataac aacattatat atgatataga cttaaataac atacctactc
     5941 atattgctat tataatggat ggaaatggaa gatgggcaaa agctagattt cttccaagaa
     6001 ctgcaggaca taaggctgga gtagaaacta taagagatat agtaaaagaa tgctcaaaat
     6061 taggtgttaa gcacttaaca ttatatgcat tttcaacaga aaactggaaa agaccgaaac
     6121 ttgaagttga cacacttatg aacttattat ctacatactt aagaaatgaa atagctgaac
     6181 ttcaccaaaa caatgtaaaa gtaactgcta taggagatat aagtgcttta ccaaaaactt
     6241 gtataagaga attaaactca gctaaggaac tgacaaaaaa caatacagga gttaacttaa
     6301 atttagcatt aaactacgga agtagagcag atataaaaaa tgctcttata gatatagtaa
     6361 aaaattgtga aagtggtaaa atagatataa acaatattga tgaagatatt ataaaaaact
     6421 atttaagcac aaagtctata ccagatccag acttggtaat aagaacaagt ggtgaacaaa
     6481 gattgagtaa ctttttactt tgggaagttg cttattctga gttttacttt actgatatac
     6541 attggcctga ttttaaaaaa gaagaactac agaaggctat atacgtatat caaagaaggg
     6601 atagaagatt tggaggactt aagtaaggag attattttat gcttacaaga attattgctt
     6661 cactagctct ggttccatta tttctatttg ttgtatatgg aggtattcct ctatatatag
     6721 ctgagacagc aatagtttat attgcattgc atgagtttta taaggcattt aaaataaaag
     6781 atgtacatcc aatattcatt ataggatatt tattttctat ttatttagct gtaaaaaata
     6841 tttttaattt accattagaa tatacttatg cagtaatttt tattttattt ttagcaagta
     6901 taatttatat gttgatgggc aaaaacaatg ttatagatgt ttcgattacg tttttaggag
     6961 ttttttatat aggagtattt ttagatttta taattataac tataaatgga tttgaaaaag
     7021 gtagtatata tgtatggctt atatttgtga tatcttttat gacagatata tttgcatatt
     7081 ttagtggtta tttacttgga aaacataagc taattcctaa agttagtcct aaaaagacta
     7141 tagaaggagc tattggagga ataataggaa gtacactttg ttgtatttta tttggatatt
     7201 tatttggcat tgatttgctt caattagcga taataggaag tataggaagt gtaatagctc
     7261 aattaggaga tttatttgca tcttctataa agagatatgt agggataaag gattatggaa
     7321 agataatacc tggtcatggg ggtatattag atagatttga tagtgttata ttagttgcac
     7381 cttttgtata tagtgcgatt aaatttttta ttagataaag attggttgga tttaaaagag
     7441 caatctataa ttgctagtaa tgaaaatagc tatgtaactt ttaaatgcag agatattctg
     7501 cagttattag ttacatggct attttcattg ttcaaaaatt tagaagacct tggggaattt
     7561 tataagaata agtattactt tataatagta tactatattt gtagtgagta tattttttat
     7621 actataacta tcattaaaaa atattttttt g
//
LOCUS       DACTGW010000182         7588 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.182, whole genome shotgun sequence.
ACCESSION   DACTGW010000182 DACTGW010000000
VERSION     DACTGW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.182"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(175..795)
                     /locus_tag="I9573_002906"
     CDS             complement(175..795)
                     /locus_tag="I9573_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAT4797512.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(1109..3247)
                     /gene="vanT"
                     /locus_tag="I9573_002907"
     CDS             complement(1109..3247)
                     /gene="vanT"
                     /locus_tag="I9573_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HAT4797513.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(3265..4071)
                     /locus_tag="I9573_002908"
     CDS             complement(3265..4071)
                     /locus_tag="I9573_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAT4797514.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(4068..5168)
                     /gene="vanG"
                     /locus_tag="I9573_002909"
     CDS             complement(4068..5168)
                     /gene="vanG"
                     /locus_tag="I9573_002909"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HAT4797515.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(5429..6511)
                     /locus_tag="I9573_002910"
     CDS             complement(5429..6511)
                     /locus_tag="I9573_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT4797516.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(6561..7262)
                     /gene="vanR"
                     /locus_tag="I9573_002911"
     CDS             complement(6561..7262)
                     /gene="vanR"
                     /locus_tag="I9573_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family DNA-binding response
                     regulator"
                     /protein_id="HAT4797517.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKAQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
BASE COUNT         2669 a         1304 c          840 g         2775 t
ORIGIN      
        1 ttattatagt tgtagaaaaa ataaataaca atattttgaa ctaagtataa ataagtttta
       61 tatattttag tttacaaaag aactaaaaat tttgtacctt aatacaaaaa ataaaacgta
      121 tcttaaaata atttagtact aaatcatttt aagatacgtt ttatttcgta caagctaaat
      181 cattttatca tctttatcat tgtgatgttt tttatgatgg aaatcataat tatgattttc
      241 aagattttta tttactctat ttccaatctt ccatgacaaa aacaccaata atataaatga
      301 tgctatcttt aaaggacttg ttataaaacc ttctatatca cttgccacgt aacttataac
      361 tataaacatt acaaattttc ctgccaacat tgctggcaca aaatctttta aatctttttt
      421 accaaaagct gatgcgatag taactaaaca accaggcata aatggacagg catacgctat
      481 aaataataac ttaaatccct gtttatccat ccaacttatt gctttttcag ttttactatt
      541 tctcaactta ttaaaaagct tactatcatt aaatctgctt attaataaga ataatgaagt
      601 tgtcccaagt cctgagccaa tccatgataa caaacatcca ataaataatc cttgtattgc
      661 agcatttgct gtaactatag caacaagtgg taaggctggt ataaagcttt ctataaatgc
      721 acttaataag ccgattattg tggctaacac ccaataattt tgagtcaaat caagtaattc
      781 ttgtatatac tgcatattgc ttttcccctt ttttaacttt aactattatt atatactcta
      841 tatgtttatt atataccaat ctcaaaaaat gaatctaata ttaaaaagta aataatctat
      901 ataagtttac agttaaggta taattatttt attatatatt ataacaaaaa aggtgatgtt
      961 tcaaaataaa aatctgaaac atcacctttt tataacttaa attgtcatgt aagttataaa
     1021 aaatatatat tagacataat attactaaaa atgtctagtc agagtaaaat aaattacaca
     1081 gtacaacaaa tattactatt taattgagtt acttctttat aacatttaac cttcttccta
     1141 ctctgctcaa taattcattc gatatactac atgagttatt tgcaacttca gaagcagata
     1201 attcagataa attatctctt cctattatta ttgccgtatt accaacttct acattaggta
     1261 tatctgtaat atcaacagca agttggtcca tgcaaattcg acccactatt gctgctctac
     1321 aaccatttat tattacgtaa ctttttccac aggataaatt tctaggtata ccatcggcat
     1381 agccaataga taatatagca attacgctat ctctgcttgc aataaagact cttccatatc
     1441 caaaactatc atctttttgt atttttctaa taagaattac ttgtgatttt aaggataaaa
     1501 caggtcgtaa atcaagttgc aatttagtcg tatcatttgg agaacttaat atcccgtata
     1561 atgcaattcc aattcttaca taattacact taagattggg ataattaaaa aaaccataac
     1621 tactttgaat atgtacttta ggaattttta tacctttttt taacagaaaa tcaattaact
     1681 tataaaaaca ttcaatttgt ttatttgtaa agtctacatc ttcatctttc agactatctg
     1741 aaacacacaa gtgtgtaaat atgccacata tatctaagta ttttaattta aatgccttta
     1801 aaattgacct cacatcatta acatcaaaac ccagtctgtg catacctgta tcaattttaa
     1861 tgtgtgattt tatgttgtat ttctgtttgt ttaaagagat agcatgatta aggtcaataa
     1921 cactctgtgt aagattgtat ttatgaagtt cctttgccct tattgggtta gtatatccaa
     1981 gaatcaatat ttctcctagt attccatatc gacgtaaatc aatcccctca tctattgttg
     2041 ctactgcaaa tgcttttaca cctatttggt ttgcacaagt cgctacttca aaagcactat
     2101 gaccataggc attagctttt acaactgcca tcaattcaca atcttctggc atagcttttt
     2161 gtatcatctt tacattatgt tccaaattat ttaaatcaat ttcaatccaa gctctattgg
     2221 tatttacctt atgttctcct ttcaagtctt tttttagagg tttaagaatc aatataataa
     2281 ttatagaagc tataaaagat gctatactta ccactatata atgaataagg ctattgtcaa
     2341 ccaagacagt ttgtattccg aatattttag aaaacatacg aataacaaca atcatcatag
     2401 gatgtataat ataaataatt aatgatgatg ttcgcaataa cctagctcga gaacctttta
     2461 aaaatgttaa ggctgtaaat aagaaataca tacatggcaa taacataata tacatgctat
     2521 catgacgctg tacccctagt ttatgtaaaa acattccctc acataacata agtaagaatg
     2581 atataagaaa tccaatcaag ctatttttag ttgacatata attagattta ttagatatta
     2641 ttccaccaag tatgaaaaat acaggtgcaa aaaatatacc atttcgtgta taatctgata
     2701 cttcaaaaat atacccatat aaattcttta atagtggtat tttttctaaa aaaccataat
     2761 aactatcacc aaacatacca attacataaa taacaatagt gattactaat gagtacttta
     2821 aacctagttt ttttacaaga aagtatgcaa tacttgcacc aataattgat gcaggtagat
     2881 accataaatg atataaagtc ccatcaaata caatgtcctt aataatattg ggcactaaat
     2941 tattcatgga aaaatatcca gtgtaaatat ttaagggaat ataaattaca atagctatcc
     3001 cgtaaataaa taatacattt tttagaaaag atttcagttt attacaatta taattatatg
     3061 ttgaaattag aaaaaagcct gatagcataa aaaagaatgg cacagcaata cgagcaatta
     3121 tactagttaa cacaaagtca gccattttac tatatgataa taatggagat gtatgaattg
     3181 caattataag taatgcagct ataatcctaa aataatcaag ccctgcataa gatttattcc
     3241 taatcattct tgttcctcca tttcttagtc ctttctattt ctccaaaatg tcacaataaa
     3301 accatcctta ttgttacccg atatttgata tacacattgt tcaggtatag agattctcac
     3361 ttcttcttta gtatgaatag gcacataaaa gatttctata attttttctt tatcttctat
     3421 ttttaaacaa tttgtatacg aaaatctctt aatatattct atatattctt caagggcgag
     3481 attcatttta ttgataatct cagaatgtgg atatccgaca taacgaaaat gccatggttc
     3541 ttgagatatt cctgttattt cttctttttt acactgataa cgctctataa aaccataatg
     3601 tagtgctgcc ttacggaact cattacaaat accattataa ggaaattcag ggcagataaa
     3661 atctataacc tctttcttca accctaaatc aatagcaagc cctgtctgat gttcactgtg
     3721 atttggaagt gctacaaact ttttagtaaa gtcttttcca ttgtcacgca gagagcttga
     3781 atatatatct tgttgttcct ctgcagtacg atatccactt acaggtacta tttcatttat
     3841 tccacatata ctttttaaaa tattttgtaa aatatttgta gcttcacgtt tcataagaat
     3901 acttggaaac cttgtatcta caggaattaa atctatatct tcagatgttt taactggtaa
     3961 aaaagcattt actagaatta gattcccatc ataaacttta tctttagtca ataagatagt
     4021 atttgtcatt ttatcttctt tatttttatc taataaaata ttattcatca ttttatgtac
     4081 acctcaatta tcttatccaa tatttcttca aaagacagtc caataccttt catcatattc
     4141 ggataacgac tatgagaagt aaagccagga attgtattga cttcattaaa aataatttca
     4201 ccagatggag ttaaaaacat atctactcta gcaaatccag aacatccaag agttctataa
     4261 ataatttttg ctgtctcttg tattcttttc tcacaatcca aatcaattct agctggcata
     4321 tgaatttttg aacttttcaa tgtatatttt tctgtataat caaaaaatcc ttttgacaat
     4381 tctatttcat ctactcttcc aacaagtaag tcatcgtttc ctaaaactgc acaccctacc
     4441 tcaaaaccat caatattttc ctcaattatt acttcattat catgttcaaa agccaagtct
     4501 actgctactt gtaactcttc ttttttataa attttactaa taccaaatga tgaacctgca
     4561 cgcacaggtt ttataaataa tggataagtt aagtcttttg acagttcagc cacattaaat
     4621 ttatctgagt attttagtaa tattgatttt ggtactttaa taccttctga atgaacaagt
     4681 ttatgtgatt tgtctttgtc catacataat gctgaagaaa gagtaccaca tccaataacc
     4741 tgtatacctg ccaattcaaa taacccctgc actgttccat cttctccatt ttttccatgc
     4801 aatacaggaa atacaaaatc aagtttagtg tatttaatat cattttcaca tgattcaata
     4861 gcactatttg gaatatgatt ctgagaaaca gcatcaggaa ttaaaaattc aataatacca
     4921 tttttgacac ggttctgcga aacaataact ggaattatat cagtatccct ttcaaaccaa
     4981 gtgttattct ggatattatt ataatctcca atataatgat accaattacc ctcccttgta
     5041 attccaattg gtataatatc atatttttcc gtattcaaat tctcaagtac tgcaaaagct
     5101 gattgtaatg atacatcata ttctgttgag tttccaccaa atataactgc aattttttta
     5161 ttattcatct ttgattctcc tttgttgatt tatttatttt tatacaatct acagaaacat
     5221 gttaaaatac tttttctatt tcaaatccaa tcaaccttca cttacaattg catctgttcc
     5281 acataatata ttttcacatt tttctattga tataattact gtatcaaaat aaaagcactc
     5341 ttgttttgat acagtaatta tatcaataac aagagtgctc tttatttatt tatcctaaag
     5401 aatttcctat ttttttatac ttaaattctt acaaatttcc tacaatcgga ataataacct
     5461 taaaggtaat tatattgttc tcgctatgag ctgttattgt cccgttatga agctccacta
     5521 tttctttagc tatagcaagt cccaacccag ctctaccact gtttgaatta cgagatgtat
     5581 ccaacctgta aaattgttca aaaatacgct caagtttttc ctctggaatc gtattgctat
     5641 gatttataaa tttaagcttt atataatcat cctcctgtac agctgttatt tcaatagtag
     5701 tatcttcaaa actataataa acagcatttc gtaataagtt atcaaataca cgttgcattt
     5761 tattaacatc acaccttatc atcatatttg gtgtaacatt cagtgtatat tttaaattct
     5821 tactcatcaa agtaggttta aactcatatg ttagttgttc aagcatacga gttaagctta
     5881 ctgtactgta ttcaagtgat atatttgata aattaaacct tgtaatttca aagaattcat
     5941 taattaaatc ttcaagacgc tctgccttat ccaacgaaat agataaatat ttttttcgca
     6001 gttcttcaga aatctgactt tcatctcgaa gtagtgtcag ataccctata actgatgtaa
     6061 gtggtgtttt taaatcatgt gcaagataaa caataagttc attcttacgt tgttctgcca
     6121 tttgtgtctc aaatttacgt tgttcaagta tatgtttaat tgagtttatt ttcttttcta
     6181 ttgccaataa ctctggagat aaagccactt ctccaacatc ttctttgata agagagtcaa
     6241 taccttgatt tatttcacta aaatattttg taaaccaatt taaataaaca cggaatatta
     6301 taaaaaacac aatcaccata gctagtatca tgaagagctc catgctatta cggaaaatct
     6361 gctgatataa attcaatgca gctttatatt ccatacgaaa agcattttca aagaatgata
     6421 ctatccagat agaaaactta ccatacaata taaaagaata taaaatccat atgaaaataa
     6481 tggctataaa tgccattaaa gctgttctca taaaaatttt atttctcact tgagtaaagt
     6541 caggattgac tttaatattt ttacttttca attgtataac ctactcccca tacagttttt
     6601 ataaatttcg ggtttttaga aggttcatga attttttcac gtaatcgccc tatatgagcc
     6661 ataactgtgt tattattatc aagatatttt tctccccata cagcttcaaa caattcttca
     6721 gaaggaacca cttttccttg atgttcacat aaataccaaa gaatagaaaa ttcaatcggt
     6781 gttaatgcta cttctttccc aaataaacta catttatgtg tttctttatt gataatcaat
     6841 cctttaatat catactcatt aacaattatc gactgttgtt cataagaatt attatatcgc
     6901 atatatcttc ttaattgtgc ctttactctt gcaacaactt ccaaagggtt aaatggcttt
     6961 gttatataat catcagcacc tatggttaaa cccattatct tgtctatatc ttcaatttta
     7021 gctgttaaca taatgatagg ataatagtat ttttctcgaa ttttctggca aatatgaaat
     7081 ccatcaatat caggaagcat aatatctaaa atagccatat ccaaatcttt agattcaata
     7141 cattctagag cttccttacc tgtataaaac ttgtatactg taaaaccttc attttttaaa
     7201 tatacttcaa ttaaatctgc tatttctttt tcatcatcca caatcaaaat attttcgttc
     7261 atagattatt tttcactctc cttattatcg aggtcatcat ttataatatt ggtaaattta
     7321 catatcaaat aattacaagc atatttgtaa ctaattttta taagaaaaga actactacat
     7381 cagaatgtgt tgatgctact attggaacac aaaaactgag attaattgtt attataaaaa
     7441 ctcccctaac acgtccttat tatcgcataa caggtattaa ctttcaataa aaatatctat
     7501 cagtcaaaca caattcaatt tattatacca gagacaatcc atataaaaaa caattaaacc
     7561 tatgtatgta accacagaca ttactaaa
//
LOCUS       DACTGW010000183         7567 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.183, whole genome shotgun sequence.
ACCESSION   DACTGW010000183 DACTGW010000000
VERSION     DACTGW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.183"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(331..870)
                     /locus_tag="I9573_002912"
     CDS             complement(331..870)
                     /locus_tag="I9573_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HAT4797518.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            complement(947..2752)
                     /locus_tag="I9573_002913"
     CDS             complement(947..2752)
                     /locus_tag="I9573_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4797519.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIYKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNVCKNKDINIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(3160..3537)
                     /locus_tag="I9573_002914"
     CDS             complement(3160..3537)
                     /locus_tag="I9573_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAT4797520.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            4388..4873
                     /locus_tag="I9573_002915"
     CDS             4388..4873
                     /locus_tag="I9573_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797521.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFDLDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(5073..5612)
                     /locus_tag="I9573_002916"
     CDS             complement(5073..5612)
                     /locus_tag="I9573_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT4797522.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEASCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVAAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(5605..6018)
                     /locus_tag="I9573_002917"
     CDS             complement(5605..6018)
                     /locus_tag="I9573_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT4797523.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(6054..7175)
                     /locus_tag="I9573_002918"
     CDS             complement(6054..7175)
                     /locus_tag="I9573_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAT4797524.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
BASE COUNT         2469 a         1338 c          809 g         2951 t
ORIGIN      
        1 tatgtctatt gttttgtcag atatattact acttttattt tctataaata tatggctata
       61 accttcttac gcataaactc tataaattta tatactttat attctttatt acccaaatat
      121 tctttattac ccaaagcctc caaaatggct taaatctttt catagcttct tacagaagtt
      181 tacatgggcg tccctccaat aagcaaaagt gcaatatgtg taccattgtg tcctacttgc
      241 gactattgag ctgtcgaaat tcgcaacttc gcatttatct gatgaaatgc actatgcaaa
      301 atattcataa catccacaac agagttaaag ttacctattc atattcactg taaaaatttc
      361 ttccacagga aatgtaattt taaccaatcc tattgagaca aactcaccac ttattttttt
      421 gaactcacgc agtaatacat cacttgcccg aaaaaaagcc tcactttcat catctccagt
      481 aagtgctatt gtgcaatggg gtacccaacg attggggtgg taccattccc aaccatttga
      541 atcaaaatct tgtagtacct catataattc tctctgaaac tgatacatac tgctattcat
      601 tataggtgat acaaacaccg ttttagtatc attaaacatg cctactgaac caatgtaagc
      661 aggtattacc ttatggtttt gtgcaaaccc ctttagcttg tctatgcaac aagcctcatc
      721 cacatcattg aaacaagcca aagtaagatg aggtcttgtt ttccattcta agtacttcgt
      781 actaattttt tcatcagcga tttttttaga taaatcaaaa agttgctttt ccgttttttt
      841 atcataatat agctctatcg catattgcat aataacacct ccaccataaa gtaaaaatac
      901 ctatctataa aaacacacaa atacctatca ttttaatatt atattactac tgttcctctt
      961 ccaattttct atatagtgta gccaaaccta tacctaaatc ctttgctatc ttcttcttcg
     1021 tctttgtatc tcttccatac ttattcaaca acttgtttat gtaagtcaac tctaattctt
     1081 taattgaaag tatcctttcc tgattctcta ctatccctcc aactatgtca tcttcaaata
     1141 ttatatttat atccttattt ttacaaacat ttccattaat attataataa tttaaaatat
     1201 ccaatggaag catatcttta tatattattc catcatctcc aaccaaattc atcatatatt
     1261 caactgcatt ttccagttcc cttatatttc ccggccaatt atagttaatc aacatattca
     1321 ttacattttc ttctatttta tttaactgaa tacctaattc taaagaatat ttagaagcaa
     1381 acttcatcat tattgcctca atatcatcct ttctttccct aagaggtggc aaatttatag
     1441 gaattacatt taatctatag tataaatcat ccctaaactt accttcattt actaatttca
     1501 ataagttttt atttgtagca gcaatcactc ttatatctaa atctataagt ttatttgacc
     1561 caattctcgc aaattttctc tcttgtaaaa ctctaagtaa ctttgcttgt aaaggcattg
     1621 acaaatctcc tatttcatca agaaatataa caccttcatt tgcaagttca aatttaccta
     1681 ctttacctcc actactagct ccactaaatg caccttttat atagccaaat agctcacttt
     1741 caaggagatt ttctggaata gcagcacagt ttatcgcaat aaatggcttg ttccatctgc
     1801 taccttcagc atgtatagcc ctagcaatta gctctttacc cgtaccactt tccccagtta
     1861 taagtacagt tgaatttgac tttgctaatt tcttagttct ctcttttact tttaacattg
     1921 cctctgaatt tccaattata gactctatat cactatttcc ccagccacta ttgacttttc
     1981 catgtccttt atgattgata tatgtcttgt caaatattat tattttgtcg tattgattta
     2041 tatatggata taccggaatt attttggcat ttatattata aattttgtta tcaatatcaa
     2101 tttctagtaa ttcatgtcca tttgaggaag attcaacaga tactatattt actatatttt
     2161 ctattatatt tttatatata tctaattttt ttagagctat attgtttata taagatattt
     2221 tgctatttat gtctagtatt ataacgcctt tttcaacatt ttcaatgatt tctatcatca
     2281 tatccatttt tctactgcta ttttttgacg cttcatgttc ttctattttc atagaaatta
     2341 aatcgcatat atgattgaca aattttaaat aattatctat ttttgcactt atttctactt
     2401 ttttagtttt atctgtacta atagcaccaa ttactcctat agttcgatta ttatacttta
     2461 ttggaactgc tattattact ttatttaggc atttcatgta atgggaacat tctatacaaa
     2521 gctgattatc acatatatcc aatacgacta attcctgacc tgtctgtata acattgtcgt
     2581 aaataaatcc ttctgtaaca acgctttcat ttatctttga tttatacaga cctgtaccac
     2641 taattcttat taatctatca tcaactattt caatatctac atctaacaca ctagctactg
     2701 tttcagtgta ttttatcatt tcttgttgaa attcttttaa tttacttaac atagtttccc
     2761 ccattagaat ttaatttacc aaattatcag tttagctata ttttaacata tagtcactct
     2821 ttaaaatata tataaaacat tacttattta tatatttaca ttaaaacaca caatattaat
     2881 ttataaaaag tttgttttat atgatgacct taattactta attataagat acagacaata
     2941 aagtaaatca caatacaata ataaaataaa aaaagctttc ctcatataac aaatatattt
     3001 ttgacataaa aatatacatt atatgagaaa agctcaatag ttaaatattt agatagattt
     3061 ttatatagac ttttttaata taaacttttt taattgtaga ctttttttat tagtttttaa
     3121 tttttattag ttttgttatt ggttttattg ttataatact tataatactg ccattgcttc
     3181 aatttctaaa actgcatctt ttggtaattg agcaacttgt acgcatgacc ttgctggtgg
     3241 attttcttta aaatatgtag catatacttc attaatatca ttaaaatcct ttaaatcttt
     3301 tacgaatact gtagtcttaa atattttatc catacttgta ccagcttgtt ctaatattgc
     3361 ttttacatta tctaaagaag cttttgttgc ttcttttggg tcagatacta actcgccagt
     3421 cttcatatct attggaagtt gacctgatgt agaaactatg tttccaactt gaattgcttg
     3481 agaatatggt ccaattgctc ctggagcttt ctctgtagct attactactt ttgacatgac
     3541 ttcacctcct attattaagt tatatttaca ggaaaataaa atttcctaaa caccaatatt
     3601 aatacgaacc tttagattaa atttcaaatc taaaacttca aataatcaat acttagatga
     3661 ttatgtttat ttgtgtcttg ttaactttta ttaaagcaat taacgtgcca aactttattt
     3721 atatataaaa ttttgtgaag tcattcaaaa tagttacttc tgaatttttt cgtaaaaaaa
     3781 tgctttaaaa ttactttttt atgatgtcaa tttttttctc atatctagaa atttaccaac
     3841 atgagaattc ttgttttgag aatttacaga ttaaagttat ttttcaatct taaattatca
     3901 agttattaca tatgaaagaa ctatagttga caatcccact attataaata ataaaacctt
     3961 tacccatacc ttatttaccc tagttatcat agagcctatt gaaaatataa cagctaaata
     4021 taataataac caaaaaatta tcaatatcta ccactgttgc tgaaaatcca aaaaactacc
     4081 ttttgatagt attacttaac aatctaatta atctcataat aaatttacaa cttataaata
     4141 aattgttata ttggccgtat ctcaacaact ttctcctttc ctagttttaa atcttttata
     4201 gaattgtacc acgtctaagt aaattttcat actattaagt cttatttcta ataaatcatt
     4261 agctctcatt ctatgcccaa taatgtaaga ttgtagtaat tttattgatt ttaatagttt
     4321 tatttaatat acttatatta attattttca tgtgaaatta ttacttatat agggggattt
     4381 ttttaatatg agcaaaagtg aattaacggc agaaacaaca gaagaaatgt tagaagtact
     4441 aagtggtaaa gattatgata ttgcatgtca tttacatgaa cttggtaaat cattagattg
     4501 taaaattgaa ccaaaaacag gtgctcgttc ttacaaaata gtatattcaa ctaagaaacc
     4561 aaaacgtagc ttatttacta ttgaatgtaa tgaaaaaaaa tggagagtta aagcaaatct
     4621 ttttcatcta aatacatata aagatgctgt ggaggaatgc tctaaaacta ttaaagatag
     4681 tataactaaa actcgtactt gtaagaaatg taattcaaag tgtattggag gttcttcgtt
     4741 tgatttagat ggaaagtctt atctgacttg cataggaagt ggtcattatt ttgcaaatat
     4801 ggaagaaatg gattggaaaa acctagaaaa attaattact aaagaaaata atattatgca
     4861 ggaatctgta tagtttttta agggtaatta ttaaaatata tgagtaacaa cagatactta
     4921 aataggcata aaaaaataaa gggtataata aggatataga ataaaaataa atgatatagc
     4981 acaaagaggc tgtctcaaaa tatagataag cctcttttta ttgttgaaat gataagaata
     5041 tattcgattt tatacaattt tatacaaaca tactaagcca acttacccat ttcatatgcc
     5101 tgtttcattg ccttacttcc cttaatatca cctacattcc aagcacctac accatatact
     5161 ataccttttt ctttagaacc atttagacat gatgtaaatc ctctaaatcc ttctattgtt
     5221 ctttccatat cagccacatt ttcatctgct gctgcaacga taaaatagaa atctttatca
     5281 caaatttcag tatatctaga gcaagttcta tcaatcatag ttttcatttg cgcattcata
     5341 gtataaaaat aaacgggagt cgctaaaaca atcacatctg ccccaatcat tttttcaaga
     5401 atttctccca tgtcatctgt ctgtgaacaa cttgcttctt tttcataaca agcaccacat
     5461 ccagtacaat agttaatatc tttatctttt aaaaatatct tctccacttg atttttagat
     5521 tctaataccc cttgtataaa ctggtcacac aataagtcag aatttccacc ttttctagga
     5581 ctagctgata ctattaatac atttttactc ataagatact gcctccattt tcttatttct
     5641 actgctcttt aatacttttt ctttctcaat aataccttct tcatatctat caatcttaaa
     5701 atttaatcgt tccaaagttt tttgcatttc ttctattctt tttgctagtt ggtttctctg
     5761 ctctattaaa agttcttttc tagctttgat agtactgctc ccttgttgaa acatagctac
     5821 atactcaatt aatgtctcaa tagaaagacc tgcacctctc atacacttta tgaaattaac
     5881 ccactcacaa tcttcttctg taaaagacct aattccactt ttatttctat ttacaggtgg
     5941 tattaaacca attcgttcat aatatcgtaa agtatccgct gaaagctcgt attttttact
     6001 aacttctgaa atcgtcatac atatcttctc ctcttgatat tgtatagatt gaattatttc
     6061 tcaaaggttt ctgctacatt ctttaaagca tctataattt taatgtgttg aggacagctt
     6121 tcttcacatt gtttacattc aatacaatct gaggcctttc cataagtttt agtataatta
     6181 ttatagtaaa ccatctgtgt tgaaaatcca gtattaagag cttgtttttc tgcattgtac
     6241 agcgcaaaat agtttggaat agcgatattt tttggacagc cttctacaca atactgacag
     6301 gcagtacaag gaactaaaat tgattcgttg ataatctcta ctgcttcatc tataatatta
     6361 tattcctctt gtacgaatgg tttgaaattt tgcatatatc ctgtattgtc taataattgt
     6421 tccatatttg acatcccact taagaccatc ataacattat ctttacttgc tgcaaaacgt
     6481 attgcccaag atgaaattga catatctgga ttgtagtcat tcaataattt ctctgctttt
     6541 tctggtatct ttgcaagtgt acctcctttt actggctcca taacaattac aggcttatta
     6601 tgcttctctg ccacctcgta gcattttcta gattgtatgc tttcattatc ccaatctaaa
     6661 taattaatct gtaattgcac gaagtctact tctggatgct cactcaagac tttatccaat
     6721 agttctgcac tatcatgaaa agaaaaacca atattcttta tttttccttc ttttttcttt
     6781 tcttcaataa acttaaagct atcaaatttt ttagctactt cataatgtga cacgcctata
     6841 ttgtgtaaaa gatagtagtc aaaatagtct acaccacatt tttctaattg ctcattaaaa
     6901 atggtttctt gctcttcttt tttctttaaa gccattaatg gaagttttgt agctatagta
     6961 aaactctctc ttttgtgtct ttttactaga gattctctaa gtgctatctc actttttccc
     7021 atgtgataca cataggctgt atcaaaatat gtaaatcccc tttctaaaaa agtgtctacc
     7081 attttattta aatgttccat atcaatagtt gtaacatcat tttcatcttt tattggtaaa
     7141 cgcattaatc caaatcctaa tttttttgcc tccattgttt tacttccccc tttaaaactt
     7201 gtttgttttt tctctctact ttaactattc ttacaagtat ataattcttg tataattata
     7261 ctttactact tggagttaac ttcaagtcaa tagatttttt tatttttaag acaaatatta
     7321 taaaaataca gttctttaat attaatccat tgcatgtttc tttcaccctc caattctcaa
     7381 acttcacaaa ctccaacatg caaagaaata tatactttaa gaaattcagt ccattaatat
     7441 tactacaaat actttttaga tatgtttaaa aagaaatttc acctaaaata tttataaaag
     7501 atttagaaat aggtcttgtg tctattttaa aatatataaa acagtggtaa agacttattt
     7561 ataagat
//
LOCUS       DACTGW010000184         7529 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.184, whole genome shotgun sequence.
ACCESSION   DACTGW010000184 DACTGW010000000
VERSION     DACTGW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.184"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            116..625
                     /locus_tag="I9573_002919"
     CDS             116..625
                     /locus_tag="I9573_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAT4797525.1"
                     /translation="MNSDERRSKLIDILKESKCPVKGGMLAEILNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHRNSKEIYDELKIIVDLGGIVKDVIVNH
                     PTYGQITAELNIHSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIETLKEETIEVITK
                     ELEDRGILS"
     gene            637..1518
                     /locus_tag="I9573_002920"
     CDS             637..1518
                     /locus_tag="I9573_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HAT4797526.1"
                     /translation="MNFLSLAKAASTNSNITKRTLENMLNNFSDNIPNIIYAIIVFVI
                     GIYISKIVRKMVSRFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGVIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFINEKR
                     ILDDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENNIKVPFVP
                     SSLLFNNMSNSNTMDKR"
     gene            1976..3247
                     /gene="serS"
                     /locus_tag="I9573_002921"
     CDS             1976..3247
                     /gene="serS"
                     /locus_tag="I9573_002921"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAT4797527.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKTRLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEYKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     gene            3567..4022
                     /locus_tag="I9573_002922"
     CDS             3567..4022
                     /locus_tag="I9573_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HAT4797528.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            4023..4114
                     /locus_tag="I9573_002923"
     tRNA            4023..4114
                     /locus_tag="I9573_002923"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4057..4059,aa:Ser,seq:gga)
     gene            4152..4416
                     /gene="ffs"
                     /locus_tag="I9573_002924"
     ncRNA           4152..4416
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="I9573_002924"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            4493..6130
                     /gene="dnaX"
                     /locus_tag="I9573_002925"
     CDS             4493..6130
                     /gene="dnaX"
                     /locus_tag="I9573_002925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAT4797529.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKYSIDENESK"
     gene            6189..6539
                     /locus_tag="I9573_002926"
     CDS             6189..6539
                     /locus_tag="I9573_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAT4797530.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            6552..7151
                     /gene="recR"
                     /locus_tag="I9573_002927"
     CDS             6552..7151
                     /gene="recR"
                     /locus_tag="I9573_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAT4797531.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            7292..7513
                     /locus_tag="I9573_002928"
     CDS             7292..7513
                     /locus_tag="I9573_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797532.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
BASE COUNT         3061 a          760 c         1397 g         2311 t
ORIGIN      
        1 tttgcaataa agtttaatat taaatagaat ataaaatcaa tattgattta taataagata
       61 aaatatagaa ttatatagta taggtaaagt ttttgattta gaaagaaggt gtaatatgaa
      121 ttctgatgaa agaagaagca aattaataga tattttaaaa gaatctaagt gccctgtaaa
      181 aggtggaatg ctagctgaga tattaaatgt aagtaggcaa gtaatagtac aagatatagc
      241 ccttataaga gctagaggat ttgaaataat agctacacct caaggatata taatatataa
      301 tcaaccatat tttacaaagc aaataaaatg taagaatcac aggaattcta aagaaattta
      361 tgatgaatta aagattattg tagatttagg tggaattgtt aaggatgtca tcgtaaatca
      421 tccaacatat ggtcagataa ctgcagagct taatattcac tctaaaatgg atatagataa
      481 ttttatgaag aaggttgaga caaatgaatt taaacagtta tcagttctaa ctgaatgtag
      541 tcatattcat acaattgaga cattaaaaga agagactata gaagttataa caaaagaatt
      601 agaggacaga ggaatattat cataaggagg agttaaatga attttttatc attagctaag
      661 gctgcttcaa caaatagtaa tataactaaa agaactttag aaaatatgct taataacttt
      721 agtgataata taccaaatat tatatatgca ataatcgtat ttgtaatagg tatctatata
      781 tctaagatag taagaaagat ggtttcaagg tttttaatca aatatggaat gagtaaaggt
      841 gtaaataatt tcattgtata tggaatatac atatctatgt tatctattat atcattgata
      901 agcttgggtg ttatagggat acaaactaca tcagttgtag ctgtattagg agctgctgga
      961 tttagtatag gtttagcatt taaagaaata ttatcaaatt taggttcagg tatgattatt
     1021 ttatttttta agccatttaa tataggtgat tatattcaag gctcaggtgt agaaggcaca
     1081 gtttctgata tacaaatatt tagtactgtt cttaaaaccc cagataataa aaccataatt
     1141 ataccaaact ttcaattgac aagtaacaat ataattaatt acacacatca aaataaaaga
     1201 aggatagatt tttcatataa tatttcatat gatagtgata ttgatgtagt taagtccata
     1261 ttgaatgaaa tttttataaa tgaaaaaagg atattggatg acccaaaacc aattataggt
     1321 ttaaactcta taggaaacaa cactatgcaa atagttgcta gaccatgggt taaaactgat
     1381 gattactggg atgtttactt tgatgtaatg gaaaaagtca aaaataaatt tgacgaaaat
     1441 aatataaaag ttccatttgt tccttcctcg ttattattta ataatatgag caattcaaat
     1501 acaatggata aaagatagaa tttcaattaa gtaaaaaagt gaatttaata taaaaaatta
     1561 aagaaaattc taaaaaaatt tgaataatag tattgaaaga tgttaatata tataatataa
     1621 tgattatcaa ataaaacaat tagttaaaaa gcggtgaagg aaaatagtaa aaaacagatt
     1681 gtattcagag aatgagtgga tggtgagaac tcattacaat ggtttttgaa tctactctag
     1741 agcttctagt taaagctaga cggtttacct acgttatagg tctgagagga ttatacgttt
     1801 tagtataatc aattagggtg gcaacgcgga taaaagattt tcgtcccttt tatagggtaa
     1861 acggaggtct ttttttattt ttcaaatttg tttaaattaa aagtttacag ctagaatact
     1921 tgtaaattaa ttggaattat aatttatatt atcaaaatta ttaggaggaa ttaatatgtt
     1981 agatattaaa agaataagag aaaatctaga tgatatcaaa aaagctatgg aaagaagagg
     2041 tgaaagagaa tttgaccttg atgctgtagt ggaactagat aataaaagaa gagaaatact
     2101 tcaagaagtt gaagttatga aaaatgagtt aaatgtaaat tctaaaaaaa tacctcaatt
     2161 aataaaagaa ggaaaagatg taacagaaga aaagacaaga ttgaaagagt tatcagataa
     2221 gataaaaggt attgatgaga aagtaaaaga agtagaagca aaaatggaat atacattaat
     2281 gagaatacca aatgttcctc atccagaggt accacaaggt gaaacagatg aagacaatgt
     2341 tcaaataaga acttggggag aacctactaa atttgatttt gagtataaag cgcattggga
     2401 tatagggaca ggtctaggta ttttagattt tgaaagagca gggaaaataa ctgggtcaag
     2461 gtttacttta tataggggat taggagctag attagaaaga tctttaatga atttcttttt
     2521 aaatactcat acagctaagc atggctatac agaagtctta cctccattta tggccaatag
     2581 aaatagtttt atagggactg gtcaacttcc taaatttgaa gaagatatgt ttaaaataga
     2641 aggattagag tatttcttaa taccaacagc tgaagttcct gttactaata tacatgctaa
     2701 tgaaatatta gatgttgcag agcttccaat taaatactgt gcatatactc cttgttttag
     2761 atctgaagca ggttcagcag gaagagatac aagaggttta gttagacaac atcaatttaa
     2821 taaagttgaa ttagttaaat ttgttaaacc agaagattct tataatgaat tagaatcatt
     2881 aactcatgat gctgaaacta tgttacaaat gttaggtctt ccatatagag ttgtaaaaat
     2941 atgtacagga gatttgggat ttacagctgc atttaaatat gatttagaag tttggatgcc
     3001 aagttacaat agatatgtag aaatatcttc ttgttcaaat tttgaagatt tccaagcaag
     3061 aagagcagga ataagattta aaagagataa aaaatcaaaa gcagaatatg ttcatacatt
     3121 aaatggttct ggattagcga taggaagatg tttggctgct atattagaaa attatcagca
     3181 ggcagatggt tctgtagtag ttccagaaga attaagacct tatatgggtg ttgatgttat
     3241 aaaataaatt tatattaatt gtatatgtag agtatgcttt gatgaagaga atatattatg
     3301 agtaaacaat aaataaaaaa actatgaaat tattataaat attaatttca tagttttttt
     3361 atgttaattt acacagcaga attttcatca taaaacacta aatattttta taaaacactc
     3421 ttgtatgaat atctaaaaat tattttagaa ctaattaaaa aaaatatcgc tatatatagt
     3481 aataattaaa taaacaaaat aaaattaagt tgaattataa aatgaattat gataagataa
     3541 gtttgttata agttttggga ggaattatgg aaagttcatt ttatatgaaa gaagcattaa
     3601 aagaagcata taaagcatat aataaaaaag aaacaccaat aggtgctatt atagttaaag
     3661 ataatcaaat aatagctaga gctcataacc ttacagaaac gttaaaagac tctacggcac
     3721 atgcagaaat tttagcgata aaacaagcat cagaaaaatt aggtggttgg aggttgactg
     3781 attgtgattt atatgttaca atggaacctt gcataatgtg tagtggcgca atagttaatt
     3841 ctagaataaa aaagcttata atagggacaa gacatgtaaa aaattcatat atagaaaaac
     3901 agcatgagtt taaattagat tattttaata ataataatgt aaaagtagct tttgatgttt
     3961 tgcaagaaga atgctcgatc atattgcaag aattttttaa ggcattgaga aaaagagatt
     4021 aaggagagat ggtcgagtgg tcgaaggcgc tcgcctggaa agcgagtata tgggtaaaac
     4081 tgtatcgtgg gttcaaatcc cactctctcc gccagaaaca atgaaagtaa ttaaatatgg
     4141 ttgtaatttt gctgtactag atggggaggt agcggtgccc tgtaacctgc aatccgctat
     4201 agcaggattg aattccatct aaaggcttat tttctgtagg gccgccctaa ataagtggtg
     4261 ttgatgctta ggtcctgcgc aatgaaagct catgaacccc gtcagatccg gaaggaagca
     4321 gcggtaagtg atcacttttg tgtgccgtgg gggtgcctaa gttgagctaa ctgttttggt
     4381 aacgcctatg gaattaagtc gatagatggt gtacagcatt taatatataa aagactaatc
     4441 ccttagataa tgagggattt ttttattaat tttgctaaag agaggtaaaa agatgcataa
     4501 agcattatat agagcatata gaccacaaaa atttgaagat gttattggtc aagatcatat
     4561 tattaagact ttaaaaaatc aaatatatag tgataatata ggacatgctt atttgttttg
     4621 tggaacaaga ggtactggta aaacatctac tgcaaagatt ttttcaagag cagtaaactg
     4681 tttgaataaa ataaatgaag aaccgtgtaa tgagtgtgag atatgtgaat ctgtattaaa
     4741 agataataca atggatgttg tagaaataga tgcagcttca aataatagtg tagatgatat
     4801 aagagagctt agagaaagtg tgaaatattc tccagccaat gctaaatata aagtatatat
     4861 aatagatgaa gttcatatgt tgtctcaagg agcttttaat gcattattaa agaccttaga
     4921 ggaacctcca tcatatgtaa tttttatatt agcaactaca gagcctcaca aaatacctgc
     4981 aaccatttta tcaagatgcc aaagatacga ttttaaaaga gttacagtta aagatatgac
     5041 ccttagaatg aaaaagattt gtgaagatga aggaattgat atagatgata aagctcttaa
     5101 tttaatagct agaaattctc aaggagctct tagagatgca cttagtatat tagaccaatg
     5161 tatgtctttt ggagaaagta agatagatta taaagatgtt gttgaattaa tgggaagcgt
     5221 aaatatagaa caattatttg aattgtctca atgtattgta gagcaggaca ctaaaaagtc
     5281 tcttgagata ttaaatgaat ttgtcttatg gggcaaagat ataagaaatc ttataaatga
     5341 cttaattgat cattttagaa atttaatggt atgtaaggtt tcatcagaat tggatgaaat
     5401 aatttcatta ccagaagaaa ctatagaaca acttaaaatt cagtctaaaa atatagatat
     5461 aaatgattta ataaggattt taaatatatt atcaataact caggatgata taaagtcatc
     5521 ttcaaatcca agggttttag tggaaataac cataatgaaa attgctcaac caatgtttga
     5581 tgaaagtaaa gaagctctta taaaaagagt tgagaactta gaaaaaatga ttgaactggg
     5641 gaactttaag tctgaaaaaa ttgggaataa taaagaaaaa gagtatgagg tcgatagaga
     5701 aatagatgta aaacaagaaa atgtagttta tgaagatgta aaaaatgagg acgttatact
     5761 tatagagtca tcttggaaga atatacttaa acaaattaaa aaagataaaa aaatgccaat
     5821 atacgcactc ttaagtgaag taaaaagttt taatgtatat tctaatatgt tgtatgttat
     5881 atttgatgat aaatttgatt ttgcaaaaac tagactgagc tctcaagata caataaatta
     5941 tttagaaaaa acaataagag atgttttaaa tagaagtttt aatgtaaaaa tagtactgac
     6001 atcagaagtt aaagatataa atcttgaagt taaagagaaa aaggacattg gtgaggagat
     6061 attaaagaac atagtgagtg aagagatatt agaaataaaa tatagtattg atgaaaatga
     6121 gagtaaataa taaaatacta ttatgatata ataataaaat gaaaataagt ttaggaggaa
     6181 tagtgtttat ggctaaaaaa ggttttggag gcggaatgat gcctggtggt ggaaatatga
     6241 acaacttgtt aaagcaggct caaaaaatgc aagaaaatat gcaaaaagct caacaagagt
     6301 tagaaagtaa agaagtagaa gcttcagttg gtggaggagc agttactgta aaagttaatg
     6361 gtaaaaaaga agttatagat ataactatca agccagaagt agttgatcca gatgatatag
     6421 aaatgctaca agatttagtt ttaagtgcag taaatcaagc attgagaaat attgatgata
     6481 tacaagcaag tcaaatgagt aaggtaactg gaggtatgaa tatacctggg ttattctagt
     6541 aggtggtatt tatgcaagtt tatacaggtc ccataacgag acttatagag gaattttcaa
     6601 agcttcctgg agtaggaaga aagactgctc aaagattggc ttttcatatt attaatatga
     6661 atactaatga tgtggaagct ttatctaaag cgattataga tgcaaaaaga gaaataagat
     6721 attgctcaat ttgttgtaat ataacagata cagacccatg tagcatgtgt tcaaataaaa
     6781 gtagagattc aagtgttata tgtgttgtgg aagatccaag agatgtagca gctatggaga
     6841 gaacgagaga gtttaaagga cagtatcatg tattaaatgg agtaatttct ccaatggatg
     6901 gaattggacc agatatgtta aaaataaagg aacttatcca aagacttggc aatcaggaag
     6961 tcaaagaaat aataatggca acaaatccta ctattgaagg tgaagcgaca gccatgtata
     7021 ttgctaggtt agtaaaacca atgggaatca aagtcactcg gatagctcat ggtcttcctg
     7081 ttggaggcga tttagagtat gcagatgagg tcacaatatc aaaagcacta gaaggcagaa
     7141 gagaaatata aaaatcgagg ttaatacctc gatttttgta gttttttgag ttaaattcaa
     7201 attaattttg atttagattt attgaataaa tttcttctgt attgtataat ttgttgtata
     7261 aatcatttaa aaattttgat aggggtgcaa catgaaacca attatgaact tagaagaaca
     7321 taatgcaatg aaagaaaaaa gagttaaaaa aatgaaatca aaagataaac ctaaatatga
     7381 tgattttgag gatataagtt tttctaataa gaaaaaaact aaaaaaaatg tacgaaataa
     7441 aaatgctgaa tttagaaata attataaaaa ttatcagtat gattatgaag aagactttta
     7501 tgaaccaatt taatattaaa taattagaa
//
LOCUS       DACTGW010000185         7423 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.185, whole genome shotgun sequence.
ACCESSION   DACTGW010000185 DACTGW010000000
VERSION     DACTGW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.185"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            33..1010
                     /locus_tag="I9573_002929"
     CDS             33..1010
                     /locus_tag="I9573_002929"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAT4797533.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKTHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1003..1971
                     /locus_tag="I9573_002930"
     CDS             1003..1971
                     /locus_tag="I9573_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HAT4797534.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2155..2745
                     /locus_tag="I9573_002931"
     CDS             2155..2745
                     /locus_tag="I9573_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAT4797535.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2764..3534
                     /locus_tag="I9573_002932"
     CDS             2764..3534
                     /locus_tag="I9573_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HAT4797536.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3671..4639
                     /gene="dusB"
                     /locus_tag="I9573_002933"
     CDS             3671..4639
                     /gene="dusB"
                     /locus_tag="I9573_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAT4797537.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4748..5227
                     /gene="greA"
                     /locus_tag="I9573_002934"
     CDS             4748..5227
                     /gene="greA"
                     /locus_tag="I9573_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAT4797538.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5242..6771
                     /gene="lysS"
                     /locus_tag="I9573_002935"
     CDS             5242..6771
                     /gene="lysS"
                     /locus_tag="I9573_002935"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAT4797539.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7080..7274
                     /locus_tag="I9573_002936"
     CDS             7080..7274
                     /locus_tag="I9573_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797540.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NIWADRVRANSYSYENKIAK"
BASE COUNT         2954 a          753 c         1507 g         2209 t
ORIGIN      
        1 gtcatgaaat tatgtcatat tagaggtgaa atatgcgaga taaaataata gagattatat
       61 taaataataa atctgaattt atttcaggag aagaattatc aaaacaatta ggggtgtcac
      121 gtgcagctat atggaaacat atgaaagcct taaaagaaga gggatataat atagaatctg
      181 taaataaaaa agggtatcga ttagcggaaa atccaactga tttattaagc ccacagaata
      241 tttactataa attagataca gagtttattg gaaaaaatat tgaacatctt gacactatag
      301 attcaacaaa tgaacatgct aaaaggatag ctataaatag tgtagatggg actgtgattt
      361 taagtgaaca tcaagatatg gggaaaggac gattgggaag aagatgggat tcaaaaactc
      421 atgaaggaat ctggatgagt attatattaa aaccagatat ggaaccattc aaagctccat
      481 ttataacttt aatagcagga gcaagtgtaa cgaaagcttt aaacaatcta ggagcaaata
      541 cattaataaa atggccaaat gatgttattt taaatggaaa aaaaatatgt ggtatattaa
      601 cagagttatc tgctgaaatt gaaagaacaa attatgttgt tcttggaatt ggaataaatg
      661 ttaaaataat ggattttgaa caagaaatag ctgacaaagc cacatcattg tataaagaag
      721 ggtatatgtt atccagagta gatattgtta ggaatgtatt gactgaattt gaaaaattat
      781 atttagatta tactgtaaat aattccaaag aaaaaacctt aaaaatatgt agagattatt
      841 ctgcaattat agataaaaat atatatgtat taaaaggaga taatagagaa ctagtgaaat
      901 gtttagacat aaatgaagag ggaaatttat tagttcaaag aagtgatgga agtataaaag
      961 aaattatttc aggtgaagtt tctataagag gagaaaaagg atatgtataa taatattttg
     1021 gatatagaag gcttaaaggt tgggcaggtt gaggatagaa atggtcttac tgggtgcaca
     1081 gtagtaatat gtgaagaagg agctacttgc ggtgttgatg taaggggttc tgctccaggg
     1141 actagagaaa ccgatttatt agaccctgta aatatggttc aaaaagttca tgcagtagtt
     1201 ttggctggag gttctgcata tggtcttgaa tccacttgtg gtgtaagtag atacttagaa
     1261 gaaaaaggaa ttggctttga tgtaggtgta ggtaaagtgc ctatagttgt aggagcagtt
     1321 ttatttgatt taggagtagg gagctttaaa tgtagaccag atttaaagat gggttataga
     1381 gcttgtgaag ttgcaaatac tgatatttta aatcaaggaa attatggagc aggttgtgga
     1441 gccactgttg gaaaaataag aggtcaggaa tatgctatga aaggcggaat aggtagctat
     1501 tctataaaat tagataatgg tctaattgta tctgcattaa ttgctgtaaa tgctcttgga
     1561 gatgtttatg aaaatggtaa ggttatagct ggtgctttaa atgataaaaa gacagaaaca
     1621 attgatacat ataaattaat gaaacaaggt gaaagtaaag gtggatttag tgtagataat
     1681 actacaattg ggataattgc aacaaatgct aagttaacca aagctggatg taaaaagata
     1741 tctcaaatgg gacatgatgg atatgctaag tctatttttc ctatccatac acctcatgat
     1801 ggagatacaa tattcacaat atcaacagga gaaatagaag cagatataac attagtaggt
     1861 tcattggctg tagaggttgt ggaaaaaagt ataataaatg cagtaaaaaa agcggatagt
     1921 gtagaaaata taatggcata taaagatata gtaaaaaaac gagaaatatg atgaaattag
     1981 catatttttc ttgtcaaatt gttgacaatg gcaactcatc ttgataaaat tattcttgtt
     2041 agagcgggca tcctacaatg gtagcttgac tcatagttat aagaaaaaaa cgaaaaaaat
     2101 taaaaaatta agtagtccgg catcttaatg agctggaacg gaggtgtatt ttttatgatg
     2161 aacacaagtg catcagtaag aaaagtaaat gtaagaaaga tgactataat aggagtacta
     2221 tcagctattt ctattatgct atctatgact ccattgggat ttattccagt tggacctaca
     2281 aaagcaacta taatgcacat accagttatt attggagcta ttatggaagg accaatagtt
     2341 ggggcagcaa taggatttat atttgggata tcaagtctct taaatgctat aacaaaccca
     2401 acagtaacat cttttgtttt tataaatcca ttagtttcaa tattaccaag agttatgata
     2461 ggtgtattag cttattatgt atatcagtta ataattaagg ctacaaataa ggtatacata
     2521 tcaggtttaa ttacaggggc aataggttct ctgttaaata cagctggagt tttaggaatg
     2581 atatatgtct tatatgctga caagtatctt catgcaatgg gtaaaagtgg ttcagcagga
     2641 aaagtaataa tggctcttgc cgcagcaaat ggggtgccag aagcaatagt aggggcttta
     2701 gtagtagctg ctgttgccac tgtacttaag aaaagcaaaa aatagaaaga aggttaatga
     2761 catatgcttc tagtatttga tgttggaaat actaatatgg ttttaggtat atataaaggt
     2821 gacaaattag ttaattactg gagaattaaa acagataggg aaaaaacgtc tgatgaatat
     2881 ggaatcctga taagtaacct atttgattat gataatgtga atataagtga tattgatgat
     2941 gttataatat catctgtagt tccaaatgtc atgcattctc ttgaaaactt ttgtataaag
     3001 tactgtaaaa aacagccatt aatagtaggt ccaggcataa aaacaggtct aaatataaaa
     3061 tatgataatc caaaacaagt tggggcagat agaatagtta atgctgtagc agggatagaa
     3121 aagtatggag caccaagtat acttgttgat tttggaacag caactacatt ttgtgctatc
     3181 tctgaaaaag gtgaatattt gggtggaaca atagcaccag gaataaaaat atctagtgag
     3241 gcgttatttc aaagtgcgtc taaattacct agagtagaat tagctaagcc aggtatgact
     3301 atttgtaaga gtactgtatc agccatgcaa tctggaataa tttatggata tgttggttta
     3361 gttgacaaaa taataagtat gatgaagaaa gaattgaatt gtgatgatgt taaggttata
     3421 gctacaggtg gattagctaa actgattgct tcagagacga aaagtataga ttatgtagat
     3481 ggttttttaa cactagaagg attgagaata atatatgaaa aaaaccaaga ataattaata
     3541 tacagggaat tgggttatat atccaattcc ctttttatga tagatttcta gctatatgtg
     3601 gtatattata atgaggaaat aagaatttaa agttaaatca caaaataaag gcaaaaatag
     3661 gagaaaaaca atgaaaatag gaaatttaga attgaaaaat aaagtttttc tatcacctat
     3721 ggcaggtgta acagaccttc catttagatt aatttgtaaa gaacagggat gtgggctttt
     3781 atatacagaa atgataaatg gtaaggcact ttgctatgat gatgaaaata ctaaaaaaat
     3841 gcttaagata gaagaggaag aacatcctgt agcagttcag atattcggtt cagaaccaga
     3901 gtttatggga agagcagcag agataatgaa tgattattca aatgagattc tcgatataaa
     3961 tatggggtgt ccagctccaa aagtagttaa aaatggagat ggttcagcac ttatgaaaaa
     4021 tcctaagctt gcagaagagg tattgagggc agtagtgaag aactcaaaaa agccagtgac
     4081 tttaaagatt cgaaaaggct gggatgataa tagtgtaaat gctgttgaaa tagcaaagat
     4141 agcagaggat tgtggaataa gcgctttagc aatacatgga agaactagag aacagtttta
     4201 tacaggcaaa gcagactggg atataattgc agagattaag aaaaatttga gtatacctgt
     4261 tataggaaat ggtgatgtat ttacaataga agattcaata aatatgttag acaaaactgg
     4321 ttgtgatgct ataatgatag gtaggggtgc tcaaggtaac ccttggatat ttaaaagaat
     4381 aaaccattat atgaatacag gcgagatttt gccagagcca actcttaacg aaaaaataag
     4441 tactgccatt aagcatttaa aacttgcagt agaagaacat ggagagtatg tagctgtaag
     4501 ggaaatgaga aaacacatcg cttggtattt aaaaggactt agaaactctg caaaattaag
     4561 ggatgaaatt aacaaaatag aagactatca agaagttgtg tcgaaattgg agtactatat
     4621 ggaagactcc ttgacataaa aataatagtg acttataata tagcgcataa attaaaaaaa
     4681 gattataaaa tttttaaaat ataattagga aaattcctaa gaaatttgtg aaggagttgt
     4741 atttataatg gaagaaaata aagagttttt attgacacaa gagggatatg ataaattaga
     4801 agaagaatta gaaaacttaa aggttgtaaa gagaaaagaa gttgcagaga gaataaaagt
     4861 tgctatatct tttggtgatt tatctgaaaa tgctgagtat gatgaagcta aaaaggaaca
     4921 agcacaagtt gaagaaagaa ttttaaaatt agaaaatatg gttagaaaag ctgttatcat
     4981 agatgaaagc aaaatagacc ttaatgttgt tacaatagga tcaatagtta aagttaagga
     5041 tttagaattt gatgaagagg ttgaatatac tatagttggt tcgacagaag cagacccata
     5101 tgatggtaaa atatcaaacg aatctcctgt agggaaagca cttttaggta gagctgcgaa
     5161 agaagttgtg gaagtgcaag ttcctgatgg agttgctaag tttgaaatat tagaaataag
     5221 aagataattt ggaggaaaaa aatgaaaaat aatcaacaaa gcaatgaaga agcgcaaatt
     5281 caagaagacc ttagtgaagt acttcaagta agaagagata aactaaaaaa attacaagag
     5341 tcaggtagag atccttttaa agaaagtaga tatgatagaa ctcattattc tatggatata
     5401 aaagataact ttgattctct tgaaggtaaa actacaaaaa tagcaggacg tataatgagc
     5461 aagagaatac aagggaaagc tggctttata gatattcaag accaagaggg aagaatccaa
     5521 tcttatgtaa gactagatgc tataggagag gaagaatata gtgtattttc aacatatgat
     5581 attggagata tagttggaat tgaaggagaa atctttaaaa ctaaaaaagg tgaaatatct
     5641 gtaaaggcta aatctgtagt attactatgt aaatctttac aagttttacc agaaaaatat
     5701 catggattaa aagaccaaga actaagatat agacaaagat atgttgactt aatagtaaat
     5761 ccagaagtaa aaaatgcttt tttaattaga acaaaagcat taaaagcatt aagagcatac
     5821 ttagatgata gaggattttt agaagttgag actccaatat taaatactat agcaggtggg
     5881 gcaaatgcta gaccatttat aactaatcat aatactttac atataccaat gtatttaaga
     5941 atagcaaatg aattgtattt aaaaagactt attgttggtg gatttgataa agtttatgaa
     6001 atgggaagaa tgtttagaaa tgaaggtatg gatttaaaac ataatcctga atatactgca
     6061 atagaattat atcaagcata tgcagactat actgatatga tggaaataac agaaaatgtt
     6121 attgcacata tggctgaagt tgctacagga agtatgatag ttaactatca aggaacagaa
     6181 ataaacttta caccaccttg gaaaagaatg agtatggaag attgtgttaa agaatattct
     6241 ggtgtagatt tttctactat aaatacagat gaagaggcac tagaagttgc tagagaaaaa
     6301 ggtatagaga taaaacctgg aatgagaaga ggagaagtaa taaatgcttt ctttgaagaa
     6361 tttggagaag ataaattaat ccaaccaaca tttataactc atcatccagt tgaagtatct
     6421 ccattatcta agagaaatgt tgaagaccca agaagaactg atagatttga ggcatttgct
     6481 aataagtggg agttagctaa tgcattttct gaacttaatg accctattga ccaaaaagga
     6541 agatttatag accaattgag aaaaagagaa ttaggtgatg atgaagcttt tgaaatggat
     6601 gaagatttct taaaagcatt agaggtaggt ctacctccta caggaggact aggaataggt
     6661 atagatagag taattatgtt attaactaac tctccatcta taagagatgt attattattc
     6721 ccgactatga aacctattga taacaatcca aataaagaag aagaaaacta agaaatatta
     6781 gcattctact aagttttaaa taaagtttag ctattaaatt agcaataaat agctaaattt
     6841 tgtttaaaat gtcagtgtag aatgcttttt atatgcttca aagattcttt tagatattta
     6901 atttataaat taccaacaaa cgttagaaat tgatattagt ttttgttgat tgagacttta
     6961 tatttagaaa atatgattga aagtaattta ttatgaaaat atttagaata atttatgaga
     7021 aggtaaaaag aataatttat gagaatacta tatataaaaa attattgggg gaatgtctga
     7081 tgtttcaagt aattaatttt ttatgtttat tttgtgttgg atttttggtt cttgatgtta
     7141 tgatatattt ttgtaaggat gcaatggata tttttgaggg tacaactaat gttaataaag
     7201 ttaagagtat aaatatttgg gcagatagag tgagagctaa ttcatattct tatgaaaata
     7261 aaattgcaaa ataggtaaaa ttatatatgc atgtattgtt tgtaatatca agggttatat
     7321 ctattagttt taattatatt tattaaaaaa accaaaagtt ataacctttt ttaaaataaa
     7381 aaacaaaaaa agtattgacg aaatattttt aagatgatat agt
//
LOCUS       DACTGW010000186         7280 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.186, whole genome shotgun sequence.
ACCESSION   DACTGW010000186 DACTGW010000000
VERSION     DACTGW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.186"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..830)
                     /locus_tag="I9573_002937"
     CDS             complement(<1..830)
                     /locus_tag="I9573_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAT4797541.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     "
     gene            complement(902..2032)
                     /locus_tag="I9573_002938"
     CDS             complement(902..2032)
                     /locus_tag="I9573_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT4797542.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     regulatory      complement(2260..2441)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2494..3387)
                     /locus_tag="I9573_002939"
     CDS             complement(2494..3387)
                     /locus_tag="I9573_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HAT4797543.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNKLNIC"
     gene            complement(3467..4369)
                     /locus_tag="I9573_002940"
     CDS             complement(3467..4369)
                     /locus_tag="I9573_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAT4797544.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESIFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(4419..5579)
                     /locus_tag="I9573_002941"
     CDS             complement(4419..5579)
                     /locus_tag="I9573_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005979864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HAT4797545.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(5735..6040)
                     /locus_tag="I9573_002942"
     CDS             complement(5735..6040)
                     /locus_tag="I9573_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HAT4797546.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGNEISFKLKDKTVKTITALDNI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(6138..7112)
                     /locus_tag="I9573_002943"
     CDS             complement(6138..7112)
                     /locus_tag="I9573_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HAT4797547.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
BASE COUNT         2298 a         1408 c          788 g         2786 t
ORIGIN      
        1 tttgaaattt gtggcactgc aagtccaata aaccctatag gtcctgcaaa tgctgatact
       61 attgctgcta atatactaga tataaacact atcaaaactc taaaaaatct tatatttatg
      121 ccaatacttt ttgcatactc ttctcctaat aaaaaagcat ttaaaggttt tattatgaat
      181 acagtcatca aaagacctaa tataccaact attataagta tttctacctt ttcccatgta
      241 aatccagaaa aacttcccat agaccacata gtaaattctt ttaatttatc actatttgca
      301 aatgattgta acacatttgt tataccacta catacatatc cggtcataat tcctattatt
      361 aaaagagttg ttatattctt taccttattt gcaaataaaa tgactataga cattacaatt
      421 aaagctccta taaaagctgc tacaaatata ctcattgaat ttacactctc aaatcccaat
      481 gtgtatcctc ccaaaatcat gagagctact gataaagtag aacctgaaga tactcctaat
      541 atatacggtt caactattgg gtttttaaaa aatatctgta ataaaatacc tgatactgct
      601 aggcatgctc cacctaccat ggttgcaagt actctaggga ttcttatatt gtatattatt
      661 ttataatgtt ctgtattatc catacctctt aatacaacat ctattacttc tttaaattca
      721 atttttacag agccaaatgc tacacacact aaaaaagtaa gtatcatact tataattaat
      781 actataaata ttgtcgtatt ccttattctc attttattga taaaatccat ttgtatactc
      841 ctcttagcaa agttatatta actaaaatct ataaaaccta tctttttgtg tcctctctgt
      901 actatttatc ataatttata taatgtttga ttttttcacc tgtagtacct gggtggaaca
      961 tctcaactaa atctactatt aattcgtccg tcttatctac tgattgataa taatctggag
     1021 caaacaccca taggtttccg ttttttacaa catccaaatc agccaataca ggtgcactag
     1081 attttataga atctaaattt ggtgcataat ctgaacttga tgagtataca aatatatctg
     1141 catcttttgc aacttcatgc aattgttcaa tacttactcg cccttcattt gaatctattg
     1201 aagcacatgc atttattcca ccagccattt ttatcatatt atccacatat gaatctttag
     1261 gagctatata agcacttcct tcatatacag atgcccatac aactttaggt ttagctttgt
     1321 tttttatttt tttacttact tcttctacct tttgtacagt tttatttagt tcactagtag
     1381 ccatatcttc tttatcataa aatgcagcta ttaaacttgt ccactccatt cttccaaatg
     1441 gattttgctc tttatactca ttgtctacaa cataattaag ccctaattct tttaatttgt
     1501 caataaattt tgtatcagcc tctgataaac cactactcac tatagttaag tcaggattta
     1561 agtctactat tttttcataa tctggtgcta aatttgaacc aacgtaatgt atttttccat
     1621 ctttcattaa atctttaatt tctttaatag tccagtactg ctcttctgtt gttacggctt
     1681 ttatactatc tagttcatct attgttctca gagaacaagc ctgtgttgta gatgctatta
     1741 atacattgtc cacaggcgtt ctaactatat tagcattttt atatttttca ggaattttaa
     1801 tcttctttgg gattaaaatc agttctcgat tatctccatc agttactttt ttaattccat
     1861 cttctaaatc ttcaacacta aatccactag catattttaa ttctaatttc ttaccagtac
     1921 tagcttgttt attctctttg tcacttttac tacaccctat aagacctcct accataaaaa
     1981 ttaccatagc aattaaaaaa aactgcttta aaacatttga tttaaatttc atctctttca
     2041 ctccaatatc ttatttttta tgagtggcta aaaaacttta ataatatatg ctttttaaag
     2101 tatacttatt aaagttatcc taaagtcatc tttaataagt ataacctttt aataaaataa
     2161 aaaacttctc ctaatttaat aaggagaagt tcatatatca actttacaaa aacgaattga
     2221 tacttcattt tattataaat tacaatctcc accctaaatt gaacttatat aatactgggc
     2281 aggtctcctg actcacggct tttcctccat cacccttccc aaaattttta taaatttcag
     2341 tggattttga tttcgtatcc gtatacagta gcgggggctg tagtgaattt tcatcacttt
     2401 ccctattaaa ctaatatagt accctagtat tattttaatt taatttttat taagataatt
     2461 gtaatttatt gttgacctct ttgcaaggtt gatttaacat atatttaatt tattcatcaa
     2521 ttttttaatt tcacatactt gtttttcatt tggttttaca aatggagcag tcatataatc
     2581 acttatttgt aaccctctga tattcatagc ttttttcatt gctggtataa atggattggt
     2641 tattaagtaa aaatccattg ctttattaat atatttttga atttctataa ccttctccat
     2701 atctctatta ttgtaagctt cagcccattt ggagcaaact tctggaatta aatttgataa
     2761 tccccctata caacctgctc ccccagacat tagattatgt ataaagtttt catcataacc
     2821 actataaacc ttaaattctg ggaactctat tttcatagta tttattaaat ctctggtatg
     2881 tcccatcaaa gttactgtat ccttatatcc aactatgttt ttatgttttc ttgcaagtct
     2941 taatgtaagt tctggactta aatcataacc agttctatct ggataattgt ataaatatat
     3001 ttcagcctct gtattcttag ctatttcact ataaaataac tcaatactat catcatcaag
     3061 agagaaataa taagggctta ttatcataac gccatctgct ccactattat gtgcatagtt
     3121 tgaaagttct atagattcat ttatacacat acgacttgta cctataaaca cttttactct
     3181 cttatttaca tgactagtta ctaaatctat aagttctttt tgcatatcca tagacatatt
     3241 aaagaactct ccagtacttc ccattatgac tagtccatct acacctccat ctataagatg
     3301 gtcgtacact tttttattac cttctttatc taaatttcct tcttcatcaa atattgtaac
     3361 aactggtgtc aaaaattctg ctttcataat ttaaactcct aaaatttatt ttaatatgtt
     3421 cattaataca tcagatttag ctttaatatt aattatatta aaacatttac ttaatgtaat
     3481 tttttaaaat tccaatactc tcaatctcac attcaactac atctccacta ctcatatatc
     3541 gaggaggtga aaatcccatc cctactccag aaggtgttcc agtagctata atgtctccag
     3601 cctctaaagt catacctttt gaaagttctg aaactatgtc tgaaacatct gatatgaata
     3661 atcctgtatt agaactttgt ctttcttcac catttaccct actacatact ttaagtttta
     3721 tgggaaaagg aactgcactt ttatgtaaaa tacatggacc taaagaggta aatgtatcta
     3781 atccttttcc tctaaaccat tgaacatgtt ctttttgcaa ctttctagca gaaatatcat
     3841 ttataacagt gtaaccaaag atataatctt ctacctcttc ttttctaata ttaacacctg
     3901 ttttacctat tactactcca agttcaactt catagtctag ttgattattt atctcaaaat
     3961 gaccatctat ctcatcttct ggacctattg cctttgtcac acgttttgta aaataaaccg
     4021 ttttttgtgg ctcttcaaaa ctatcatcaa agtgagtttg agtttcttca agatggtctt
     4081 tatagttcac tcccacacat aagatatcat gtattggttt ttctatagga acatgtacag
     4141 tacatctctc tcttttaaaa acatcataat tatagaatat actttcattt ataatagttt
     4201 tcaacttaat aatatcttca ctagatacat tttgaattaa ttcatttaaa tccttaaaat
     4261 ttctactgag atttaaagag ttaacatcta taataaataa ttcatcttta gaaaaaactc
     4321 cagttctttt acattcattt tctccttcag taaaacttac aaacttcata atctcaccct
     4381 ttattaaaat tctatgtatt tattttctta cttaaagatt atacatagtt gcatactcta
     4441 gctattgcca tttcagtata tccaagggac tctgcttttg attgttttcc atttgctaca
     4501 tctacaacca tatttaacaa atcatctgtt aattcttcca ttgtttgtgg accatagata
     4561 actggactag catcaaaatc catattatct tccatatttg caaaagttat tttatttcca
     4621 gttattttta ttactggtgc tattggattt cctgatggtg ttcctcttcc tgtactaaat
     4681 accactattt gtgctcctcc tgctaccata ccagcaacag atgaagcatc atttccaggg
     4741 gtatccatta taacaagacc tttcttatca atttgtttgg cataatcata tacttcactt
     4801 atttgtctat gacctccttt atgtatacaa ccaagtgatt tttcttctaa acttgttaaa
     4861 ccaccagcta tatttccagg agatggatta ccttctctaa cttcttctcc aaccaatttt
     4921 aatgaatttt catatctatg tactatatgt aatatttttt cttttacttc ttcatttact
     4981 gctctcctag ctagtatatg ctctgcacct ataaattcag tagtttcact taaaattgat
     5041 gttgctccta aatcgacaag tttatcactt aattcaccta ttaatggatt tgctgctaat
     5101 ccacttgttg ggtcagaccc accacattca gttcctagta taagttcaga tattggaaat
     5161 tcttcttttc taagcaaaga agcttcttgt gccatttctc tagcataacg aacagctttt
     5221 tctatagttt ttaaagttcc atgttcttct tgtattacaa gagtttttat tggcttattt
     5281 gttctctctt ttattgcatc aactaccaaa tcattttggc aattttcgca tcccaaagat
     5341 acaactatta caccatatac atttggattt gctgcataac cagccataac atccatagtt
     5401 aattgttggt ctgaatgtac ttgtgaacat ccattctgat tattaaaggt aactgcccct
     5461 tgaacttgtg atgctattat tctagttgta tctgatgcac acacactcgt aggcaatatt
     5521 aatatgtggt ttcttatacc aactcttcca tctggtcttt tatatccata aaatttcata
     5581 ctgatttcct cctaaaatag tctatatact ttttataatt tttatatatt ataattttta
     5641 ttacactcac taatcaaaca gctaaaatcc tatttaaaaa ctatttgaca ctaaaagcaa
     5701 tgaatacaac ttttataact ctataaatat tattttatat attagtcata acttgtttat
     5761 ccaaatcctc tctaacactt ctaacattat gaatatgtac atgttgacca ctttttattt
     5821 cctgatttgc ttctcctata tgttcaccat atttagtaac tttatcacct tgggccatat
     5881 cttttatagc caatttatga taaatagtta tattatctag tgctgtaatc gtttttactg
     5941 ttttgtcttt taatttaaaa ctaatttcat tacctttagc tattgcttca atagctactg
     6001 caacattatc tttttcatca attatcactg catttatcat tattcttgct ctcctttata
     6061 aataatataa ttttattaat atcctaaaat ctaattaaag aatttattat tttaccaaca
     6121 actcactgta atataaatta agctgttatt gtaccatcct cattttgttt tgggaactta
     6181 ggacatccat acttcttagc ccaccaacta gttataagag gtactaatat tgcagtgaca
     6241 actactgcgg ctgccacttg tgttgtagct gttgcaacat atggttgcca tgatgggtca
     6301 actaatgcaa cagcagcagg tacagcaatt gcatttccag cggttgtagc tacagcccat
     6361 cctgcatatc ctggacgttt tcctataaat ttatcacaga atactataaa tatacctcct
     6421 acaaatacag atattaatcc aagtaatact cctgataaac ctccttttag tatacttgca
     6481 agatttatgc tactaccaag tgcaaaccct acaaaaggta cgagtaaatt acaccctggc
     6541 tctaaaaact ctttcatttt tttgtcaaaa tttcctatta tcataccaac aagtattggc
     6601 actattgcag ctaataatga caacaatggc acatttgcaa gacctgaagt cccaagagct
     6661 attaatgtaa aaaatggtcc atcatttaaa gtaagtaaac tcatagttgc acaatctgtt
     6721 tcatctccat atgaagtcat caatgaaaga tatatactac cattactatt tgtaactgca
     6781 cttataacag ccaatgttgt aagaccaagg acaccatcca ttccaaatag ctttcctata
     6841 agtataccta tcaaagcacc tatagcaaat ttagaaccaa gaagtatacc acctctcttt
     6901 aaaacttttg gcatttctct aaattgaagc tgagttccca agcataccag ttgaacccca
     6961 attatagttg cagacccaac acttgaaaat actgctgtcg taaacgaacc tatttgtaca
     7021 atatctgggc aaaaggtatt aatgaaagct gctataaaca ttggcactat catcatacct
     7081 gcaggaattt ttttaataaa atccattatc ataaagttaa tcctcctaaa acatgatatt
     7141 taattactct tattaagttt ttagctttaa ttctaacggc cgtttcggtt cgtatttaag
     7201 aactaagttt ttaattacga atctttgaat taatgatagc attttcctat ttatgagtca
     7261 ataaaaaaaa taaatttttg
//
LOCUS       DACTGW010000187         7274 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.187, whole genome shotgun sequence.
ACCESSION   DACTGW010000187 DACTGW010000000
VERSION     DACTGW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.187"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(127..654)
                     /locus_tag="I9573_002944"
                     /pseudo
     CDS             complement(127..654)
                     /locus_tag="I9573_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            938..1504
                     /locus_tag="I9573_002945"
     CDS             938..1504
                     /locus_tag="I9573_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT4797548.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
     gene            complement(1695..3731)
                     /locus_tag="I9573_002946"
     CDS             complement(1695..3731)
                     /locus_tag="I9573_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HAT4797549.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLKNVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(3905..4582)
                     /locus_tag="I9573_002947"
     CDS             complement(3905..4582)
                     /locus_tag="I9573_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAT4797550.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSRLYFDLSTPGPNHNTKIDINPPS
                     FEKPLKK"
     gene            5277..5462
                     /locus_tag="I9573_002948"
     CDS             5277..5462
                     /locus_tag="I9573_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAT4797551.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(5503..>7274)
                     /locus_tag="I9573_002949"
     CDS             complement(5503..>7274)
                     /locus_tag="I9573_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HAT4797552.1"
                     /translation="NLENLQFLNNNLKSIDLSHNKKLKSLNCTNNKLKSLDLSNNVFL
                     KNLSCSGNNLSKLNIKGLTSLEYLGCAKNKLQDLDVSTNIKLDSLLCGKNSLTKIDIS
                     KNKELNSLECSGQYIEKSIIKNGENIVVDIGIENSKLKELYPQNYNISTGFLTWENLS
                     DFPNSISYSLEYEYGPVNDTETMDVNISIDKLVKCRIRYIDYDGSIINEKEISDNSSI
                     NEIQINPQRENYEFSGWRNNKDEKIYTSEQVSKLVFDDSTDFIATYKRKSTGSSGSSG
                     GTSSKEDFNIAPSKEKITGADRNETSVKISQKGWNKADNIVLINDSSISDALSATPFA
                     KSKDAPILLTKNNNLNKLTEKEINRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRI
                     SGNDRYETSIKLAKELDKNSNLSKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQND
                     DMKDIKDLIANKNISKSYVIGGESLFNNKEVNNTLPSVTKIAGSDRTETNSKVISHFY
                     SKDTLNDLYVAKNGMNKQDDLVDALSVGVLAGKTESPVVLVGNGLDDSQKELIKNKKF
                     KNITQIGGNGNEKAFNEVENLVK"
BASE COUNT         2395 a         1152 c          810 g         2917 t
ORIGIN      
        1 ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca tataggtttc
       61 cctatatgct gactatttta gctactaact catgactaaa gtcacaagtg tgcatagcta
      121 ttttttaatc aaattattta ttttattact tttagaacgt cttccatact ttttaatctc
      181 acgattgatt atcagatgag caagatttag aatgattcct tgctttccat ttgaaccaca
      241 tagtataaga ccagataaat tattactgta atccattata tatctttgac ttgcaaatga
      301 acccatgctg tgtccaaata gataaattgg caaatcttca ttttctttct ttataatatt
      361 tgttaaagta tacatgtcct ctacaagaca tctaaatcct tctttttcag ctaaatgtcc
      421 aacattttct attatctttg cagtttttcc atgacctcta tggtcattga tatatactat
      481 ataaccattt ttagtaagta ccttggcaaa agtctcatac ctttgtgcag tttcagccat
      541 tccatgtgca atttggataa ctgcctttgg tttttttata ttttcatctt cccatttata
      601 tgtatatata tctaaacctt cctctccttt gaaagtaaag tttgtacact tcataattat
      661 ccctccagat tgattttatt cagtatatta atttatatat tatattacaa gtattttcaa
      721 gtcaaaatct ttctaaaaaa tttaagaatt attaattcct catagaaatt atcattggta
      781 catagaagtt tttacataca attatacaat aatttaatta atagttacat aaaaaagtaa
      841 tactttaata tattttgaca ccatattgac attagaatta attcatgata ttctgttttc
      901 gacaaaacaa cttctaaaaa taaaggagat atatttcatg gattattcaa atgaactaaa
      961 agagctcttt ttaatgaatc aaacatatgc aactcttttt acccttacaa acaaaataca
     1021 aatagaagga gataaatatt ttggtatact tacttcaaga cagtatatga ctattttatc
     1081 aattcttcat ttaccagagg aagaaacaac attaaataat attgcaagaa aaatgggtac
     1141 aagtaaacaa aatataaata gactagttgc taatcttgaa aaaaatggat atgttgatgt
     1201 aattccaagt cctcatgata agagagcaat taatgttaaa gttacagact taggaaaaaa
     1261 ggttatggta acatgtagta ggactggaat aaactttatg gcagatgtat ttcacgaatt
     1321 taccaaagat gaattagaga ccttatggag tttactaaaa aaaatgtatc gttttaatgg
     1381 tgaagaacaa gatggctttg aagaagatgc taattttatg gaatatgaag aaatagataa
     1441 aattaaatct gaagcacttg aagaatttgc aaaaagaaga aatagggtaa ataaaaacga
     1501 ttaatactat cgcatctata ataccacttt tttatagtac aatattttat ataactataa
     1561 tgtcattttt attttttaac aaaacaatca ttacatagat taggtgttct taggaactct
     1621 ttttctgttt tttgtacaaa aaaaggagat gattagactt ttattttaaa gcatctcctt
     1681 tttcgctatt atgattattc tactcctaaa atcatatctt ttaatttttg taaatcatta
     1741 attggttgac tacaactatt actttgacac acgtaataac tagtcttatc atccttcaat
     1801 ctatagttat ttaaaaaacc tataatagtg ttttcttcat tgtatttctt tgctaaaaaa
     1861 gttgcatttg gtataaaatt ttcacttatc aattctttaa atgcaattaa atcagaatct
     1921 tccttaaaaa tacatataat ttccttagta gaataaagtt caaacatcaa tgaaagcata
     1981 taaaagctgt agccagttgg agaactcttt acattatcca catacagttt taattgttta
     2041 tatgacattt cttcaagtct attatctcca gtaattttcg caagtcttat aagattgtat
     2101 aattgaacag aatttcctga tggaatagct ccatcataca aatcttttgg tcttgctatt
     2161 aaattttcac tatctttacc atatatataa aatccacttt tttcataatc ccaaaaaagg
     2221 ttgatacagc tttcattcaa atttaaagcc ttttctaaat atttcatatt aaatgttgac
     2281 tcataaagct ctatatacgc ccagattaaa aaagcataat catctaaata tgctaaatag
     2341 tcggaacttc catctctata tctagctaat aatctacctg actcatttac aagattatta
     2401 tttataaagt taagacactt atttgaatac tctaaatata tgtcattttt taaagtacta
     2461 tatgcctttg ttaaagcaac tatcatcaat gcattccatg atgtaagtat cttgtcatct
     2521 ttatgcaaag atgttctttc ttttctatat tcaaatacct ttttactaag gtcagctatc
     2581 ttttcattat gtctttcata ttctttattt tttataagat taggaatact tttaccttca
     2641 aaatttccac tactagttat atcaaaataa ttattaaaga aagttccatc ttcttcaccc
     2701 aaaacttcaa ttatctcaag aggattaaat gtataaaatt taccttcttc accttcacta
     2761 tcagcatcct gtgctgaata aaacccacct tctttatcct tcatctctct aactacataa
     2821 tctatagttt ttatagcaat ttctttatat aactctttct ttgttatttt atatgcatct
     2881 aaaaaagcta tagtaagcat agcattatca tatagcattt tttcaaaatg aggagccaac
     2941 cactttttat cagtagagta tcttgaaaac ccaaatccaa tatgatcaaa caagcctcct
     3001 ctatacattc catctaaagt tttttccacc atttttaaca catctttatc tttttctatg
     3061 ctataatatt tcattaaaaa cataagattc tgaggacttg gaaatttagg agcattacca
     3121 aaaccaccat acttttcatc ataaatggcc ttaaatactc taactgatga actcaacatc
     3181 tcttttgata aatcaccctc agtatttttt acaccaaaat catccttaag agcttctatt
     3241 atttcatctc cagattttat taatatatcc ctacttgtat tccatttttc agatacattc
     3301 tttaataaat ctataactcc aggtctatta taccttgaat attttgggaa atatgttccc
     3361 gcaaaaaaag gttttttatc tggtgtcatt attatggtca ttggccatcc accactacct
     3421 gtcatagctt ggcaaactgt catatacacg ctatctacat caggtctttc ctctttatct
     3481 acttttattg ccacaaagtt tctattcatt atttcagcaa cttcttcatc ttcaaaagat
     3541 tctttttcca taacatgaca ccaatgacat gttgaatatc ctacacttaa aaatattggt
     3601 ttatcctctt cttttgcttt tttaaaagcc tcatcattcc aactatacca gtttatagga
     3661 ttataagcat gctgtaaaag atatggagat ttttcattta ttagattatt aggttttcta
     3721 ttactgtcca aaataccacc tcatttgtta ttttattata tgttttgcaa taaacaaata
     3781 gatatacact tataataagt gtatacccat ttttaactat acatatttaa tttttcatat
     3841 aattaattct tctagtaaat aatttttata actactaaaa caaaacaaca taaaaaatat
     3901 caaattattt ttttaaaggt ttttcaaaag aaggaggatt aatatctatc ttagtattgt
     3961 gatttggccc tggagtagac aaatcgaaat ataatctaga atcttctgta actccaaaat
     4021 ctctatttga acctgtatct ttaactttat ttaaagcatc tctaaataga gcattatgtg
     4081 cttcttctct gttaagtaag aaatctattg tttcttttac atatttatca tctatttgtc
     4141 tatacaagta ctcataaact accttagctc tttgttcaga agctatattt gaaagtaagt
     4201 cagctactaa atcccctgta actgtaacat aattagcagt ccaaggagct cctgatgagt
     4261 ttattaagac tggtgataga ccagttaata catgtgtttc tatttcgcct gtattaactg
     4321 cattataatc aacatcatgt ccatttaata aatttatagt ttgtgctacc atttccatat
     4381 ggctaagctc ttctgctgct atatcaagaa ataaatcttt tatttgaggg tcttttattc
     4441 taaaactttg ggacaagtat tgcatagctg cttttaactc tccatttgca ccacccaact
     4501 gttcttgcat taaaacagca tattgaggat ttggtctttc tacttttact tcttttaata
     4561 atgctttatc gtgtttaaac atatgaatac ctccgtaaat tattattcaa aattattatt
     4621 tacttaagtt gtaatattat tctaggttat ttgtttattg ttgttatttt ttacaagtgt
     4681 tggtatttca atgttttatt aaggtcagtt ttgttgttta tgttttatta tgtttaattt
     4741 aaatttattc tattatttat tttcggtggc aaaatggtgg caagtttggt tttgtcacca
     4801 tttattttta tttgtaaaaa atatagttta tcaatataac tttattgaaa aatatgtaat
     4861 atttattgac tttcacgtca cgtgatgata taatataaat atagaaagga ggtaagaaag
     4921 tggggagatt agaaagaaga aaaaataaaa gagaaaacaa atttaataaa attaaaaatg
     4981 ctttctcttt cacattagtt ttaattaatc tagtactcgc aatactaaga ttgattaaag
     5041 aattataagt ttcaatccta ggaggaaaat accttccttc taggtagctt ctttctaata
     5101 ttataacaca ttttctaatt taatatgaat aataaatttt atagaatttg tatgattatt
     5161 cttggtatca ctatagtttt aaatattatt aatataataa taagttttac ttttaaagct
     5221 cttattgctt tggtatttac tttattattg ctggcattta ttaatagtag aaaggaatga
     5281 tattattgga gcaaagaaaa cttagaattg gatttaataa aagtggcaaa ggctctttta
     5341 cacccagatt gatacttcca atgtcatgga ttaaagagat gaatatctct cctgatgaaa
     5401 gggatgttct tgttactttt gaggatggaa aaataattat tgaaaaaact gaaaatgaat
     5461 aaaaaaggag gtagtaacta tttccagtta ctacctttca atctatttaa ctaagttttc
     5521 tacttcatta aatgcttttt catttccatt tccaccaatt tgagttatat ttttaaactt
     5581 tttattcttg attaactctt tttggctatc atctaatcca tttccaacta atactactgg
     5641 agattcagtt ttacctgcaa gcactcctac tgataaggca tctactaaat cgtcttgttt
     5701 attcattcca ttcttagcta catataagtc attaagtgta tctttgctat agaaatgact
     5761 tattactttt gagttagttt ctgttctatc agaacctgct attttagtta cagatggtaa
     5821 tgtattattc acttctttat tattaaataa agattctcca cctattacat atgattttga
     5881 tatgttttta ttagctatta agtcttttat atctttcata tcatcatttt gattagtaag
     5941 aagaattggc atttcctctt ttgctgatat agctcccata cttactgcat cagctagtcc
     6001 cttttctcca tttaccacta ctacttttga caggttagaa tttttgtcta attcttttgc
     6061 aagttttatt gatgtttcat atctatcatt tccagatatt ctaattacat ttaatccttt
     6121 tttctttaaa tcagacacta ctttttcatc aacagacttt aatccaccca ctatgtacac
     6181 attttttgct tctaatctat ttatttcttt ttctgttaat ttatttaaat tattattttt
     6241 agtaagtaat attggtgcat ctttagactt agcaaatggt gttgctgata aagcatccga
     6301 tatgctagag tcatttatta acactatatt atcagcttta ttccatcctt tttgacttat
     6361 tttcactgat gtttcatttc tgtctgcacc tgttattttt tccttagatg gtgctatatt
     6421 aaaatcttct ttacttgaag ttccaccaga agaaccactt gatcctgttg attttctttt
     6481 ataagttgct ataaaatcag tactatcatc aaaaactaat ttacttacct gctctgatgt
     6541 atatattttt tcatctttat tgttcctcca accactaaac tcatagtttt ctctttgagg
     6601 attaatttga atttcattta tactagaatt atctgatatt tctttttcat taattatact
     6661 tccatcataa tctatatatc tgattctaca tttcactaat ttatctattg atatatttac
     6721 atccatagtc tctgtatcat ttactggacc atattcatat tccaaactat aacttatgct
     6781 atttggaaag tcagaaagat tttcccatgt tagaaaacct gttgaaatat tataattttg
     6841 tggatataat tcctttaatt tcgaattttc aattcctata tctacaacta tattttcacc
     6901 attttttata atgctttttt ctatatattg accactacat tccagtgaat ttaattcttt
     6961 atttttactt atatctattt ttgttaaact atttttccca caaagtaaag aatctaattt
     7021 tatatttgta gatacatcta aatcttgtaa tttattctta gcacatccca aatactcaag
     7081 acttgtaagt ccttttatat ttaatttact caggttatta cctgaacaac ttaaattttt
     7141 caaaaaaaca ttattactta aatcaaggga tttaagttta ttgttagtac aatttagaga
     7201 tttcaacttt ttattatgac ttaaatctat actttttaaa ttattattaa gaaattgtaa
     7261 attttctaag ttca
//
LOCUS       DACTGW010000188         7217 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.188, whole genome shotgun sequence.
ACCESSION   DACTGW010000188 DACTGW010000000
VERSION     DACTGW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.188"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(13..264)
                     /locus_tag="I9573_002950"
     CDS             complement(13..264)
                     /locus_tag="I9573_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797553.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGTKFINKKR"
     gene            353..1084
                     /locus_tag="I9573_002951"
     CDS             353..1084
                     /locus_tag="I9573_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAT4797554.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            1143..2054
                     /locus_tag="I9573_002952"
     CDS             1143..2054
                     /locus_tag="I9573_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT4797555.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEVGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            2105..2917
                     /locus_tag="I9573_002953"
     CDS             2105..2917
                     /locus_tag="I9573_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAT4797556.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            3027..3719
                     /locus_tag="I9573_002954"
     CDS             3027..3719
                     /locus_tag="I9573_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAT4797557.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            3806..4747
                     /locus_tag="I9573_002955"
     CDS             3806..4747
                     /locus_tag="I9573_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797558.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            4784..5941
                     /locus_tag="I9573_002956"
     CDS             4784..5941
                     /locus_tag="I9573_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT4797559.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRHFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            6093..6653
                     /locus_tag="I9573_002957"
     CDS             6093..6653
                     /locus_tag="I9573_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT4797560.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            6681..7172
                     /locus_tag="I9573_002958"
     CDS             6681..7172
                     /locus_tag="I9573_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797561.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
BASE COUNT         3031 a          647 c         1249 g         2290 t
ORIGIN      
        1 tatttttgat aactatcttt ttttatttat aaattttgtt cctaaatatt ttttgatatc
       61 ttcttcagga gaaaagtcat caataagtat ttctttccca gtgtcttcaa atactacaag
      121 aacttcacag tcaccttgca agaaaaattt tctaactgta tcttctttaa cttctctaac
      181 ttttattaca ttcttttttt ctttttcaaa gtcttttgtg taataacttc ccatcatatt
      241 aattattttt gtatatctat tcataaaata cctccatatg cgattaaatt taaaaaatat
      301 gttataataa ttgtttgtat taacttaata attataacaa ggagtaataa taatgactag
      361 tataaaaaat aaagatattt taaattgtat agattattcc attaaaaata agctctttga
      421 caaactaaat gatgtatata agtccttgcc agtaggaaat tgttctggat gtggaaattg
      481 ttgtatggag tcagtaggta taaatttaat tgagtttttg aatatatttt gttatcttga
      541 agatagagtt gatttaaaaa aaagctgtat aagtaaaata ttagattatt attttgagga
      601 gtatacaaga aagaacccat gtccatttaa agatactaat aatagatgtc taatatatga
      661 agttagacca ttaaattgta ggatgtttgg acattggaaa aaagaggatt ataataaaaa
      721 tttagataat gttattgaga aaaataataa ctatagagag ttaatgaaaa agcaatatgg
      781 atttgaaata aatgatgaag tagtaaatta caggatagat tattgtgata gatttattcc
      841 aaataaagat tatttaagta aatctaagag attaagcttt tttgatgagc ttatgatatt
      901 agactcaaat atttattcaa gtggaaatat agatatagac tttagggata gaggtatagt
      961 tgaatatttt atagagtcat tactttatag aaatttagca tataatgtga aaataaggat
     1021 atctaaagaa tacaaaataa gaaaaagaac tatagataga ataaaaagaa ttgttcttgt
     1081 atagttttat attagtaagt aataaattgt atcataaata ttgtcaaaat gaggtgagga
     1141 aggtgaatct tacaaaagta aaaggaaata ccttttttat aaaaggtggc acgaatacag
     1201 gaatatatgt atttgaagat aacacagcac ttatgataga cccagggtta tgtggttcaa
     1261 gacctaggag agtaaataaa gtattagatg aaaataatat acaggttaga tatattataa
     1321 atactcatgg tcatgatgac cactatggag cttgtaatca atttaaagaa tattataaag
     1381 atgtgtgtat aatgtcatct gaatacgaca aattatatat agaaaatcca gaattgtttt
     1441 caaagtatat tgttggggga aagagcaatg tatttatgga taatatctta aaaaataaaa
     1501 tgttggatga tataaagata gataaatcta tagaagttgg aaaaattgaa ttaaacaatg
     1561 aaatgtttga tataattgag tttcagggtc atacacctgg tagtatagct gttttgacga
     1621 aggataatgt gatttttgtt ggcgatttat tgataggaga tgaaattcta tcaaaatacg
     1681 atttcttgtt tctatatgat atacaagaac aaataaattc attagaaaaa ttaaaaaata
     1741 tagattttga gtacttagtt ttagggcatg gcaagcaagt tatatcgaaa gaagattctt
     1801 ataaattaat agaaaaacat ttgaatgctt taaaaaaata tttatatcaa ataagagagt
     1861 tgcttgtaga accgaaatct ttagaaattt tattaaaaaa cattataaat aacaataatt
     1921 taagcaataa ttataaagag tatcattttt ttaaatcttc gttagtatct gtcataagtt
     1981 acttagttga tttagaagaa atagggtata tattggaaaa tggagaattg ctttactata
     2041 caaaaacaaa ataaattatg ataaaataaa gaaggtcata aaaaacaaat aaagggtgag
     2101 caatatggaa tacgaaaaat ggaatgagat actagcacct tatgaacatg cagtagagga
     2161 attgaaaata aaatttaaaa atataagaaa agaatattta gcaaaaggtg aatattctcc
     2221 catagagttt gtcacaggca gaacaaaaaa aatatcttcc ataatatcta agttaaaaag
     2281 gataaatgca aaagatatag aaactgaaat tgatgatatt gctggtataa gaataatgtg
     2341 ccagtttgtt gaggatatat atgctatagt tgatttgata aaagtaagaa atgatatgac
     2401 tataatagga gaaaaagatt atattacaaa ttacaaagat agtggttata gaagctatca
     2461 tgttataata aaatacccta taaactccat tgcgggttca aaggaaatta tatgtgaaat
     2521 acaaataaga actcttgcaa tgaatttctg ggctacaata gaacattcat taaaatataa
     2581 atatgaccat tacatacctg agactcttgc tgaaaggctt agaagagcat ctgatgctgc
     2641 ctttctttta gaccaagaaa tgagtgaaat aagagaggac ataatgaaag ctcaggctat
     2701 gtaccaaatg aagtctatag ctattagaga tactctaaat agaatacaag agttatatga
     2761 cttgggtgat acacataaag ctatgcagta ccaaagaaaa cttgatagaa ctgaaacaga
     2821 taaaaatata actgaaattt tggaattaaa acaagaaata gatatattac tagagcaata
     2881 taaggatact aaggtagatg ataaagtagt tcagtaaaaa actacttgta gaaaatttgc
     2941 taaatattaa tggggctgcc tcttaatgga aaataatttc taaaaagaga cagcctattt
     3001 agtgtaatta agaaaggaat gatattatga gtttgtatgc tataggtgat ttacattttt
     3061 caacatcagt caataagcca atgaatatct ttggaagtaa ttgggatggt catgaaaaga
     3121 aaattataga taattggaaa gaagttgtta aggaagaaga tatggtctta gtactaggag
     3181 atacatcttg gggaattaat ttatcggaag ctaaaaaaga tttagatata ataagcaaac
     3241 ttccaggtca gaaaatactt ataaaaggaa atcatgatta ctggtggaca accgttacaa
     3301 gtttaaataa gctatatgaa gacatgcgtt ttattcagac aaatttttat gaatataaag
     3361 attatgcaat ttgtggaggt aggggctgga tatgtcctaa tgatgttaaa tttgatgaga
     3421 ctgatgaaaa agtttataaa agagaagagc atagacttag actatcatta gaatctgctc
     3481 gtaaaagtgg tcactcaaaa ataattgtga ttactcatta tcctcctacg aatgataaat
     3541 tggaagaatc tttatttaca aagttatttg aagaatataa tgttgaaaag gtgatttatg
     3601 gtcacttgca tggtaaagaa tcttttaaaa tgggattgaa ggggattcga aatggagtag
     3661 aatatacatt agcatcttgt gactatacag aatttaattt aataaaagtt cacgattaaa
     3721 aaacataaat tggtaaatac tataagatac atgattaaaa ataaatattt gagggatttc
     3781 ggttttgaga aaggagacac gacttatgag gtggatatta gtttttatat ttttatatct
     3841 tattccttta gtagtgatat tcagaaatta caagaatttt aagaggtctt gtatctacag
     3901 tagtatttat gtggtattag ctacaacaat aatgataagt aatatttata tgagtggact
     3961 taataaaata aaagagtcta tgtattatca caattatatt tctgatgaga catatgatga
     4021 aaagtatgta tcaaatttta aggaaaaaga aagtaccgat ttaaaacagg ataataaaaa
     4081 gaatgaagaa atatcaaata ataaaaattt aaacaacaaa caagatagaa atatacaaaa
     4141 taaagaaagt aatgaagaag gaagtaatga tataaaagaa aagaataatt ctgatagtaa
     4201 tgtttcagtg aataataaat cagaaaaaca agaagataat acaaaagaaa ataataaata
     4261 tacaagtaaa gaaaaaaata gtgaagatat aaaaaaaata gatatggaaa gtataggaat
     4321 ttttaagaaa gaaatttata atatagaaag agcagcttta gttccaatga gagaatgtat
     4381 accttacact aaggatatag caaaaaatat aactaaatta tctagtataa aaaaagatgt
     4441 tacaaatgcc agaaacaagt gtaaagaagt tgtaaaagaa tatgaaaata tgcagattcc
     4501 agaactttct aaggagaagt atacagaaac attgagtaat gctagagatg acgtaaagaa
     4561 agcatatgag cttagagaaa aatctatgga aagtgctttg aaattaataa atactaaaag
     4621 ccctaaatat atagggaaga taacagaata tcttgattta tcagataagc agatagaaag
     4681 ttttaaaaat agattgaacg atttaaaaga agaaataaat aattcagaaa caaataatat
     4741 taattaataa gatttaaaga tataagttaa aagggagatt atgatgaata aacagagtat
     4801 aggaaaacta aatatgtttt ttatggggat tactcaaaga aaccttgata ataggcattt
     4861 ttttattgag ttagaggtga tttttaaatc tggaagaaaa gagtttaaag gaattgcaat
     4921 agaggaagat aataaatata aatttaattt taaaggaaaa tcagatttat atacgtttaa
     4981 tgagttgctt aagaatattt ctaaagaggc agaaaattat gatggattag tatttaaata
     5041 tgttgagcga ggaactattg ttgtaattga aggagacaat aagaaggtaa atgtaaaata
     5101 tttagataat aaagaagaag tacctaaaat agatgaattt acggcatctc aaataaaaaa
     5161 cagagattat tatgtaaaag taggacaagc aaatgcactt ttaaaagaaa ttggagtact
     5221 tacaaaagat ggtaagataa aaaatgataa aataagaaaa tacaatcaga tagaccattt
     5281 tgttgaattg atagatagta ttttaaaaga aataaaggat aaagactgta taactatctt
     5341 ggattgtgct tgtggaaaat cttacttaag ctttgtctta aatttttata taaaagaagt
     5401 attgaagaaa aactgttatt ttataggaat agattattca gatgtagtta tagaagcatc
     5461 aaaaaatatg gctaaaaact tgggttataa aaatatgtca tttataaaag aggatttgac
     5521 taattataca cctaataggg atgttgacct tgtaataagt ctacatgctt gtgatacagc
     5581 tacagatatg gcaataggtc taggaattag agcaaaatct gaagctatag tagtagtacc
     5641 atgttgtcat aaggagttat tagggcaata tagatatgaa gctatggaac caatattaaa
     5701 acatggtgta tttaaagcta gatttgcaga tttgataact gatggtttaa gaacattgtt
     5761 attggagggc aatggatatg atacttctgt agtagaatac atatctccac ttgatacacc
     5821 aaaaaattta atgataagag cgataaaaac taaaacgaat aatgacaaag cattaaaaga
     5881 atataaagaa ctgaaatcac aatttggagt ggaaccaacc ttagaaaaac ttatatatta
     5941 gttaaaaatg taaaatttac gaaaagcttg cttaaatatg caagcttttt ggtattatat
     6001 aggtatagtc atatccagag ttttgattta taattaaagc tgtgctataa tatttgatta
     6061 atcaaatcaa taaatgttac atggaggtaa aaatgaacaa attgaatata tgtattgata
     6121 tagatggaac aataacaagc ccttatcatt ttcttccata cctaaatgat atctttaata
     6181 aaaatataac tgaagcagag tgtataacac ataattggga agaattgtat ggttcgggaa
     6241 taagagatgt gtatgcggag tttaataata agtatataca ctcatatgat gaagcaaaga
     6301 ttgtagaggg tgctacagag gtaattagca cattatctaa aggacataat ttatcttttg
     6361 taacggctag acatgagtgt ttaactgatg taactaaaaa ttggctatcg agacaaggat
     6421 tttcagatat agatgtttac ttattaggaa gtgattataa agttgaaaag gcaagagaac
     6481 tgagttgtga catatttata gaagataatc ctttaaactc agtgcaacta gctgatgatg
     6541 gtgttaaagt aatacttctg gatactaact ataataaaga tgttaaacat caaaatatag
     6601 ttagagttag taattggata gatattgaaa agattataaa acaaggtatt taaagatttg
     6661 tagattgaaa gggggagcat atgatagatt taaaaattga aaatagaaaa ggaaaaatcc
     6721 atgtaaagag cagtgaaata aaagacattt tagaattacg tccagacttc gaatatgtac
     6781 aagatatatc aaatactata aatcaagaaa atatattggt atttgattgc cagttgacag
     6841 gaaattcttt tgatatggat gatttagata tagaagaaat attggaagag ttaggtgaag
     6901 aaatagatga gtcttatttc agtgttttat ttgaagatgt aagagcgtat ttaaaagatg
     6961 ctactgatga gatagaagca gacttacaag ataattactt atttgataat ttgagatgtt
     7021 attttgatat atacaatata aatcaagaat ttacagattt taagtttgta tttttagttt
     7081 cttttaaaga tataaaaatt tcttcattag caaacctagc taagattgtt tctaagagac
     7141 agcttatagg tgcatctaag ttttatagtt aaaatgaaat attattataa taaaagctag
     7201 tagccttgta aaaatat
//
LOCUS       DACTGW010000189         7204 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.189, whole genome shotgun sequence.
ACCESSION   DACTGW010000189 DACTGW010000000
VERSION     DACTGW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.189"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            83..634
                     /locus_tag="I9573_002959"
     CDS             83..634
                     /locus_tag="I9573_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4797562.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            720..1652
                     /locus_tag="I9573_002960"
     CDS             720..1652
                     /locus_tag="I9573_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797563.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            1707..2570
                     /locus_tag="I9573_002961"
     CDS             1707..2570
                     /locus_tag="I9573_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAT4797564.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            2817..4679
                     /locus_tag="I9573_002962"
     CDS             2817..4679
                     /locus_tag="I9573_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAT4797565.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            4682..6148
                     /locus_tag="I9573_002963"
     CDS             4682..6148
                     /locus_tag="I9573_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAT4797566.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            6172..7008
                     /locus_tag="I9573_002964"
     CDS             6172..7008
                     /locus_tag="I9573_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAT4797567.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKKFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT         2648 a          764 c         1316 g         2476 t
ORIGIN      
        1 ataaaaagtt tctattaaat tactaagttt atttaataat caaaataatt tatcttatat
       61 tagtatgtat tagggggtat ttatgcaaag tttaaatgaa ataatcgctg aaaatttaaa
      121 aaagataagg aaagaaaaac atcttagttt agacaagatt gcacaactta gtggtgtaag
      181 taagagtatg ctatctcaaa ttgaaaagtg tgaggtaaat cctactatat ctactttgaa
      241 gaaaataact aatggactaa aaatctcatt tacatcattg atggagaggc aggaaagtga
      301 catagaattg attcaaaaaa gtgatataga ccattttatt gaagataatg gaaagtatat
      361 aagttatcca atatttccat ttgactccaa aagaagattt gagatattta tgattgaaat
      421 agaagaaggt ggaaatttag attcaaatgc tgaacttcca ggaacacaag agtttataac
      481 tgtttttagt ggcgaagtaa ccattaaaat aaatggagaa gattacattg tatctagtgg
      541 aaattctata agatttaaag ctgatgtatc acatatatat agaaactcag gcaaaggtat
      601 agctaaaatg agtatggtag tatattatat ataaataatt taatggaatt agagtgatat
      661 ttagatgatt ttaggtatga agatatatta tatttaagtt ataggagtgt gatttatgga
      721 tgaataataa aaaagcagtt tttttctttt gtagtgattt aaagaaagac ccagtagcca
      781 gtaatgtgat aaagtgttgt gaagaaataa tggatttaaa tcaaactgat attgttattg
      841 atgataattt tgtattagaa tttaaggata aaaacaataa cttatttcat tttgtaaaaa
      901 caaaagatgt aataagtcat aattataaat attatcttcc aattttaaat agacatttca
      961 gagattatga ttttgcagga gttgtaaatt ggcatgaggg agaaaatgct ccagaacata
     1021 tcctaacagc tcatacaatt ggagatgttc caacaggaga atttggaaat tcaaatccaa
     1081 gatactttaa aaatctaatc aatgctattg aggatataaa aaatgaaaat ctacttgatg
     1141 aatttactac attaacagaa gcaactcact ggtctggaac aacatatggt gaagaatcta
     1201 aattaattac agaatattct gtacctatgt tggatattga gataggaagt agtagtgata
     1261 gttttaataa tagtatagca atacaggtat tggcaaaatc attaattaga gtatttgatt
     1321 gtgatgaacc tttgaagact ttattatgtg ttggaggggt acattttgag aaatctttct
     1381 cagatattat aaaaaataaa gaatataaca tatcaattgg acatgtattg ccaaatcaat
     1441 ggattgtaag tggaatgtat gatgatgaat cgggatttga gaaactagaa aagtgtataa
     1501 atagtattga aggtggaatt gattgtatag tttttcatga taaattaaaa ggtacctata
     1561 aagagcaatg ccgtaaacta ggtgaaaaac ttaatgtacc tgtatttaaa cataaaatat
     1621 taaaaaatcc caaagactta cctatatggt agtcttatac agttaatata acaataagtc
     1681 ggattaattt ttaagcgagg tcaaaaatga tattatctta ttttgcatta ttaattagag
     1741 tagtgctatt agcttttgaa agagtagttg taagattatt aggtgatgaa gaaggagata
     1801 tttataaaaa tatagcttca tcatttctat ttttctttat agggggagta tgtttacttc
     1861 catttagcat aatggataga gttagtgatt ggtcgttttt aataccatgt tatataagta
     1921 gtttagtcta ttcaattggt tcagtagcgt atgtaacgtc tttagcaact ggggaagttt
     1981 cacttgttac tccaataaat agtttaaatt cattgttttt actgctttta tcagttatat
     2041 ttttaggtga aggtttaagt ttagcaaaag ttgcaggaat cataattatg attggtggag
     2101 tgtttatatt aaaaaaagta agctcttttt taaagaatgt ctctgtaatt tttaacaatt
     2161 taccatgtag attaatgttt ttatacatat ttttgcagtc agtaggaaga gtattggata
     2221 aggctttttt tgtacaagta tctcctatat tatattcaac tatactgtat tttttcgtag
     2281 gtttaaattt atttatcttt ttagcattta aaaagaaaca aaaaataatt tgtgagattt
     2341 ttagtaataa aaaggggctt tcaattttaa gtggtgctat taatggatat tcttatttag
     2401 cattattaat tgctttaaat aatctagaac ttagtatagc agagccattt tctcaagtat
     2461 ctatgataat aactttaata cttgctcatt ttatattcaa agaaaatata aaagaaaaaa
     2521 taccaggttc aattttgata ctaattggtg ggtggctact gttactttaa ttgacataaa
     2581 aaatgaatta taaaggtgct attagttagt atcagaaaat ttaaaaaagt attgacaatt
     2641 cttttaggca aatgttaata taataataca aaatagtata aatagaagac atgttactgg
     2701 taatgcaggc aggatcgaat atattgcttt gatttttcaa ggtgtttata taggttctgc
     2761 cttttttgca tcttgaatgg ccattcataa aaaattatgg agaaggagat tacaagatga
     2821 attataaaaa aactgccatt gacattattg aacaagttgg tggttcaaaa aatattttag
     2881 ctttagaaca ctgttcaact agactacgct ttaagttgat tgatagaagt aaagtcaatc
     2941 aggatgcttt aaaaaaagta caaggtgtta tgagtatagt taatggggca caatttcaag
     3001 ttgtcattgg aaatagtgtt gttgatgtgt atgatgaaat tttaaaagtt tgccctttag
     3061 atacaaatga tagtactgta aaaactgaag aaaaaataaa cttaggaaca agatttatag
     3121 aatttattat taatattttt caaccactga tttttgctat tgcaggagct ggtattttaa
     3181 aatcactgtt gatgttatta gcaatgttta atattttaaa atcagattct actatatata
     3241 cattattagt tgctatttca gatgcaacat tctatttcct acctcttatg gtagctgtaa
     3301 ctactgccaa tgtattgaaa tgtaatcgtt tagtagctat tgccgctgta ggatacttat
     3361 tgcttccagc aacaacaact gctttaagtg aaggtgtcga actatttgga tttacaattc
     3421 caaacattgc ctataatgca caagttttcc cggctatctt atgtgtgtct tttttggcaa
     3481 caatggagaa aatttttaac aagtattcac caaaaccaat tcgaactttc tttgttccta
     3541 tgatgtcttt ggcaattact gttccaatta ctctaactat tttaggtcct ttaggatata
     3601 acgttggtac aatttttaca accattattt tattcttata taacaaaatg ggattcttag
     3661 ttgtaggatt acttgctgct tttcttccat ttatggttgc aacaggaatg cacaaagcac
     3721 taatacctta ttcaattaat acaattggaa agttgggtta tgaagcttta tatatgacat
     3781 cttcattagc ccacaatatt tctgaaagtg gtgcttgttt tgcagtagcc ttgcgaacaa
     3841 aagatgaaac attaaaacaa acagcattat ctgcaggaat ttctgccttg atgggaatta
     3901 cagagccagc tttatatggt attactttac aacataaaag agcaatgtta ggtgttgtat
     3961 tctcaagtgc aatttctggt acatttttag gattatttgc agtaaaagga tttgtaattg
     4021 ttggaccagg gcttgccagt atgagtatgt ttattgaccc aaacaatgga aacaatttga
     4081 tttttgcgag tattggtttt gttattgcaa tattaggctc gtttattatt acactgatta
     4141 tttggaagga agatacaaat acagtagagt catcaattgg agatgttgtt gaagaagagc
     4201 tagaagaaga aaaagaagta aatatcttag taactccagt agaaggaaaa gtaatagatt
     4261 taagtaaggt taatgatgaa ctatttgcaa gtaagacttt gggagatgga gttgcaatta
     4321 taccgaccaa tggaaattta tacgcacctt gtgattcaga agttgtgatg ttatttgaaa
     4381 caaaacatgc aattggttta agaacaaaaa atggggctga gattttgatt catattggaa
     4441 taaatactgt aagtatgaat ggagatggat ttaaaacatt tgttaagaca ggcgataatg
     4501 taaaagaagg agatttatta attcaatttg atttagacaa aatctcacat gctaatttag
     4561 attcaactgt tatgattgta aacaataatg gagcagatta tgcttataaa gtgttaaacc
     4621 agtcttatgg caatgtaaaa aaaggaagta tcttgtttga tgtaaaggga ggaatataaa
     4681 tatgagaaag tttccagaag gatttctttg gggaggagct acagctgcta atcaatttga
     4741 aggtggttgg aatttaggtg gtaaagggtg gtctgtatca gatgtagcaa aggcacactt
     4801 tgatgcagat gtgaaagact ataaatctaa taatgaaatc acaacaaaag atatagaaga
     4861 aggattagca catcctgaag atgaagtaaa ttatccaaaa cgtcatggta gtgattttta
     4921 tcatcactat aaagaagata ttgctctaat ggcagaaatg ggatttaaga catatcgaat
     4981 gagtattgct tggtctcgta tttttccaaa tggagatgat aaagagccaa atgaagaagg
     5041 tttacagttt tatgatgatg tatttgatga gttgataagt tatggaattg aaccacttgt
     5101 tactatgtca cattatgaac caccattaaa tatcgtttta aactatgatg gatggtattc
     5161 acgacaagtt atcaatatgt ttgttaggta tgtagaaact atctgtgaaa gatataaaaa
     5221 caaagtgaaa tattggctta catttaatga agttgattct atgattcgtc atccgtacac
     5281 tactggtggt ttagttcgag accgttttaa ggataaaaac tttgaggaag ttatttttca
     5341 agctatgcat catcaatttg tagctagtgc ccttgcaaca aaaatttgcc atgagattat
     5401 tccaaattca aaagtagggt gtatgttaac aaaattaacg tattatccat atacatgtag
     5461 accagaagat gttttggcaa ctcaacaaaa aatgagaagt atttatgcat atagtgatac
     5521 gcaggttttt ggtgagtatc cagtatattt actttcgtat tttaaaaata ataacattca
     5581 aatagcaaaa gaagaacatg atgatgaaat tatgaaaaag tatccagtag attttatttc
     5641 attctcttac tatatgtctt cttgtgaggc agctgataca actggattag atattacacc
     5701 tgggaatacg ttattagctg taaaaaatcc ttatttagaa atgagtgaat ggggttggca
     5761 aattgattca ataggtctac gtatatcttt gattgaatta tatgatagat acagaaagcc
     5821 tttatttata gttgaaaatg gattgggagc taaagatatt ttaactgaag ataaaaaagt
     5881 tcatgaccaa tatagaatta attatttaaa agaacacttt aaatgtatgt tagatgcgat
     5941 tatcgaagat ggcgttgaac tttggggata cacttcatgg ggttgtattg atttggtcag
     6001 tgaatcaacg aaacaaatgt caaaacgtta tggatatatt tatgtggatg ctgatgatta
     6061 tggaaagggg acatataatc gatataagaa agatagtttt tattggtata aaaaagtaat
     6121 tgaaaataat agtatagatt ttaaatgacc aatagaaggt gagcaaataa aatgaactac
     6181 ataataaaga aagttttgaa ctcgagcgtt gttttagttc atgatgcaaa gtataatgaa
     6241 tttattttgc tagggaaagg aattggatat ggtaaaaaaa caggagaata tatttgtggt
     6301 tccaatgata atcaaatgtt tgtccctgtt gaaaatacaa aatcaaaaaa atttttagaa
     6361 ttaatggatg atattcctgt cgacatttta aagataacac aggaaataat agtagaggca
     6421 gaaaagttat tgaactcatc ttttaataaa aacttatact taatattagc agaccatttt
     6481 aactttgcaa ttgaaaggat gcgtaaagga ataaaaatta caaatcgagt attttgggaa
     6541 ataaaaaatt attatccaaa tgagtttaag gttggaatgg ttgcaattta tatggtagag
     6601 gagagattgg gcattcaatt accagaggaa gaagcagcta acattgcttt ccattttgca
     6661 aatgctatgg cttcagatgg aaattcatat gatacgatta agtatgccaa agtaattgga
     6721 gaaataatta atatttttgt tttttctttg aatagaacct tagataaaaa gagtatgcat
     6781 tatatgagat ttataacaca tataaaattt tttgtggaaa gatttttttc agatagtatg
     6841 ttaagcagtg gtgatgattt attatttaat caaatgaaaa ggtcaaatcc aaaagaaatg
     6901 ttaattgcat taaaagttag agatttttta gaaaagaaat atggaaaaaa gttaacaaat
     6961 gaggaaatag ctttcttggt tgtccatatc gcacgagtta agcaatagag tgaattacta
     7021 atattatctt atacgtctac atggtagaaa attgaaagaa ggtaaagtat ataaagaaac
     7081 tgagacatat tattttgttg ttcattatgt ttattttatg agtgtatcct gttaaaaatt
     7141 taagttaata tttataatta aaatatacaa atagttaaaa taagtttatg tggttatgaa
     7201 ttta
//
LOCUS       DACTGW010000190         7193 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.190, whole genome shotgun sequence.
ACCESSION   DACTGW010000190 DACTGW010000000
VERSION     DACTGW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.190"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            88..927
                     /locus_tag="I9573_002965"
     CDS             88..927
                     /locus_tag="I9573_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAT4797568.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            1195..2676
                     /locus_tag="I9573_002966"
     CDS             1195..2676
                     /locus_tag="I9573_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT4797569.1"
                     /translation="MKKNRLIIKNISIAFIVLFFFSVFTFFYIGNISRVLEYETNDIM
                     TVTITGWIILSFLFLCIIIYILYSKAKSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIIANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFLKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            2709..6146
                     /locus_tag="I9573_002967"
     CDS             2709..6146
                     /locus_tag="I9573_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAT4797570.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPIFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEISKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENILGGIIITEFNDDFTIKYTNSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIIDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSEMVKTISNSIIHKDNKEVFLNTFLCKNLLSAYNKNITEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIKLKLKAERDTLTGLYN
                     KGTTENLINDFLNSDEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKHLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENTKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNNCIYQNELENNISDNNIKLANK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSER
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            6164..6910
                     /locus_tag="I9573_002968"
     CDS             6164..6910
                     /locus_tag="I9573_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAT4797571.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNSIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(6859..7179)
                     /locus_tag="I9573_002969"
     CDS             complement(6859..7179)
                     /locus_tag="I9573_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797572.1"
                     /translation="MEKIMNFLKENSTFYFATVEDNKPRVRSFGFIMDYEGKLYFGIV
                     KYKTSYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAK
                     NSLV"
BASE COUNT         3020 a          692 c         1087 g         2394 t
ORIGIN      
        1 taatgctgta tataatatta caaatgcagt cagtgaaaat tttaaatttg actttaaaga
       61 aaaataatct agggggaaat aaaaataatg aatattgtta ttgtaggact aggagttata
      121 ggaggctctt ttgctaaagc attaaaaaaa gctggatatg aaaatgtatt tggagtagat
      181 gtagatttag aaactttaaa aaaagcagaa aaagcaaaaa tcataaaaaa aggatgtact
      241 acaggtaaag agttatttaa aaaagctgat ttaattatac tatctatata tcctagatta
      301 gttgtagatt ttctaaataa taataaaaac ttttttaaaa aagggactat aattactgat
      361 actacaggaa taaaagaaac acttatcaat gatgtgttgc aaattattcc agatgatata
      421 gattttatat ttggacatcc aatggcaggt agggaaaaaa agggtattga ttttgcaagt
      481 gaacaagttt ttaatggcgc aaattatata attactccaa ctggcagaaa caacataaaa
      541 aatttagagt tagttgaaaa tttaatttta gagattggat ttaaaagagt aaaaaagtta
      601 acttcacaaa aacatgatga gattatagct tttacttctc agttacctca tgttatggca
      661 gttgctctta taaatagtga tgaagaaggt agagatacag gtaaatttat tggagatagt
      721 tatagagatt taactagaat tgcaaatatg aatgaagact tatggagtga acttttttta
      781 ggaaatagag ataatttact aaaagttata gaaaactttg aatcagaagt aaatttagta
      841 aaagaagcta tatttaacaa tgataaaaat aaattgatag agtattttaa aaaatcatca
      901 ataagaagag aagctttaga aaaataaata ttaatattta agctatatat agatgggtat
      961 aaatgtaagc gatagtgtat ttaattgtat taataaatat ggattcaata taattaaata
     1021 tgctattgat atatttgtac ccttaactat tgttatcttt taaatgataa tgatatttat
     1081 ttaaatgctc tttattaaca atttataaaa cttaattgta aaaaatactt attaatagta
     1141 aaataaagtg aatcaattat attataattg ctaaagagag tgaggtgtag ctatttgaaa
     1201 aaaaatagat taataataaa gaatatatca atagcattta tagtactatt cttttttagt
     1261 gtatttacat tcttttatat aggtaatatt agtagagtat tagaatatga aacaaatgac
     1321 ataatgacag ttacaattac tggatggatt atactttcat ttttattttt atgtattata
     1381 atttacatat tatattcaaa agcaaagagt caaaaaacta tagaaaaagt agcatatacg
     1441 gattttgtaa caggttattc caactggaga aagtttgaat tagatgttac aaatttatta
     1501 aagaaaacat cacaaaataa taaatatgca atggtcatat tcgatattga taaatttaaa
     1561 gcaattaatg atatatatgg acataaaaag ggcaatttaa tattaaaaga tattgcagat
     1621 actttaaatg aattgactaa tatcaatgaa acttttgctc gtgtaagtgc tgataatttt
     1681 aatatattat taacatataa caaaaaagag gatataataa atattattaa aaaaattata
     1741 gcaaataatg aacttgtaaa cttatctttt ggaatttatg aaatcaaaga taaggattta
     1801 tctgtaagtg tatatagtga tagagcttca cttgcaaaat catcaataaa aaataatagt
     1861 gatgtaaatt ttgcattttt taatgataaa ttaagagaga aattattact tgaagataag
     1921 atagaaaaag aaatggaata tgctcttgaa agtgggcaat tcgtaatgta tttacaacca
     1981 aaatataata ttaaattaga taaattttgt ggaagtgaag ccctagtcag atggcaatat
     2041 acagaaaaag aagtcatata tccaggtgat tttattccta tatttgaaaa aaatggattt
     2101 attagaaaaa ttgatatgta catattagaa caagcttgta aagaaatccg gtctttattt
     2161 gataaaggaa tttcaccttt acctatatct gtaaacttct caagagttga ttttttaaaa
     2221 aaagatttta ttgaaaacat agtaaatata tgtgatagat ataaaatacc atatagttta
     2281 atagaaattg aaattactga atctagtatg tttggagata ctgatactct atttaatgtt
     2341 agtagaaacc tacaggatat tggttttata gttgcaatgg atgattttgg ctcgggatat
     2401 tcttctgtaa atatgcttaa aaatatacca ttaaatgtaa taaaattaga tagaggattt
     2461 tttgttgatg ataaagatgt tgataagagt caaattgtta taaaaagcat agtttctctc
     2521 attaagcaat taggaataag ggttgttgct gaaggaattg aaacaagaag tcaaatagaa
     2581 atgttaaaaa aagcaaattg tgatattgtt caaggatatt atttttcaaa accattacca
     2641 attaaagaat ttgaaaaatt agtctataaa atatagtcag cgagtcttaa atgggaggtg
     2701 gtgattaagt gataagagca aaatcgttaa aaaatgtact gagtattgtt ttaatatttt
     2761 tatttacaat aatttctttt tctgattttg catttaaaac taatgtccag gaagaagaaa
     2821 atagtaaaat taatttaagg gaaatatccc tacaaagttc caatgctata aaaataaaat
     2881 tagatagttc tctatccttt attaaaagtg tggcaaatat ggtttcttat tttaatacta
     2941 tagataactc aaaaatagtt ccattattag ctaacatatc aaaagaatct gactttcaaa
     3001 gaatctctat tgtatctcct gatggaataa gtcataccag cgatggcaaa attatcaatg
     3061 tgtcaaaata tcagtatttt aagaaagcta ttaaaggaga aagcaatata tctgaagttg
     3121 tgaaatctcc aattgatgga aaagatactt ttgttatagc tactcctata tttaataaga
     3181 ataacaaaat aattggtgtt ttatacggtt cttactacaa tgataaatta gctgaattta
     3241 tagatataac aagttttaat agtgaagggt acatagatat atttgaatct agtggtcaat
     3301 ttgttttaaa atcttcacat acaaacaaac tgattaagtc aagtagtaac atatatgata
     3361 tatttaaaaa agcttctttt gatgatggtt atagctataa aaaaatatat gaagatacaa
     3421 aaaataacaa gagtggcttt acctcatatg aatacaaagg agaaaatcgt tatgctactt
     3481 atacacccat tggtgtaaat gactggtatg taatttctgt gataccacaa tatactgtaa
     3541 gaagaaacat tgataaaata aatcaattag cattttctct tattttcaaa gtattcttat
     3601 tgtttattat gcttataata tatattattt ataaaaacaa taaatctcag aaagatatac
     3661 taaaagctaa caaagaaatc tcatctatta caaatagcat tcctggagga gtacaaaagt
     3721 gtaccctggg tgaaacatgc aatattaatt ttttaagtga tggatttata gagttgtttg
     3781 gttatacaag agaagaaata aaaacattgt ttaataatga tttttatagt ttaatacata
     3841 aagatgatgt tgaaagaata aaaaaagaaa tttctaaaca actttacaaa aataaaacta
     3901 ttgaattaga atataggatt ataacaaaaa atggtaatac agtatgggtt ttagataaag
     3961 gagaacttat aacagaacct gatggaaaag aatatttttg ttgtgtaact gtagatatta
     4021 ctagaacaaa acaattagaa caagagatgg aacaaaaaaa tctagaactt gaaatgatat
     4081 cagaaaatat tttaggtggt ataattataa cagaatttaa tgatgatttt actataaaat
     4141 acactaactc tggttatctt tcaatgattg gatatacacg tgaacaatta gaaaatgaac
     4201 ttaaatacaa agcttgtaat cttgtttttt atgaagatag aaagaaggct cttgaaagta
     4261 tacaacatca attaaacaca aaaggaatag ctgtatatca acatagaata agaagaagag
     4321 atggtaatat tatctgggtt tctataaaag gaaggcttat tattgatgat gatggtattg
     4381 aaaaaggtat ttggattcta aatgacataa cagatttaaa aaatgcagaa caaaaactta
     4441 aaataaatga agaaagattt agaattgcat taaagcaaac atcaaatact atatttgagt
     4501 atgatataaa aacaaagtca cttttaaata ctagtaaaga gcttgataaa tataatattt
     4561 ataaaattac taaaaacgtt ccatattctc tagtagaatc aggagttatt cataaagatt
     4621 ttagtgatga ttttataaac atgtatgaaa atgtaataaa tggagcaaaa aaagcaagtt
     4681 gtgtagttaa gatgaaactt ccagatgaaa attatatatg gaataaagtt accttgacta
     4741 ctatttttga tgaagatggt aatcctgtaa gagcaattgg tatacaggaa gatataagca
     4801 aacaaaagga agcagagata agatataatc aagaaggtaa gtatcattca gctatattgt
     4861 cggaagcaat gataacctat gaaataaatg tatcaaaaga tacttttatc aaaggaaatg
     4921 aaagttggca aaaaccattt ggaattaagc ctgacaataa ttattctgaa atggtaaaaa
     4981 ctatatcaaa cagtattatt cacaaagaca ataaagaggt gtttttaaat acatttttgt
     5041 gtaaaaattt attatctgca tataacaaaa atataactga aataaaatta gaatatcgaa
     5101 gaatgaatca agaaaataaa atgatttggg tcatttgcac aatgcactta ataaaagatt
     5161 ctattacgaa tgatataaaa gcacttgctt atgttaaaga tattgacaag caaaaaaatg
     5221 aagaaattaa attaaaatta aaagctgaaa gagatactct tacaggatta tataataaag
     5281 gtactacaga aaatttgatt aatgatttct tgaactctga cgaagcaagc ttaggaatgc
     5341 atgcattttt aattgttgat atagataatt ttaaatctgt aaatgataat ttaggacatg
     5401 tatttggaga taaagtatta tttgaagttt ctaaacacct aaaacctata tttagaaaag
     5461 atgatatagt aggtcgaatt ggtggagatg aatttatagt attcttaaaa aatattcagt
     5521 cttttgagaa taccaaaaag aaagcagaaa aaatatgcga tatatttaga aattcttata
     5581 cggaaaataa aaagacatat aaaatatctg gaacagttgg aatttcattt gtaccagaac
     5641 atggaactgt atttgaagaa ctttataaaa aagcagacat agcactttat tatgcaaaga
     5701 gcaaaggaaa agataagtat gcactttata ataattgtat ctaccaaaat gaacttgaaa
     5761 ataatataag tgataataat ataaaattag caaataaatg tgagaaatcc aaaccagtac
     5821 ttaaagaaat tcttgattct attgataatt atatttatat agtgaatcca aatacttatg
     5881 aacttctttt tgtcaataat aaggtgtctc gtttggattt agagataaaa ttaggagaaa
     5941 cctgttataa atcactatta aaatctgaga gaccatgtaa taattgccct cttaacggat
     6001 tagaagaaaa taagcattca aaattcaaaa tagagacaaa tacatttggt aaaaatatca
     6061 gtatagcaag ttggattaat tgcatagatg gagaatacaa ctgtcttata gagtgtatag
     6121 attcttcttt atacaaaaat aaataaaaaa ggactatgaa gatatgttaa atagtataac
     6181 tttgatactt ggtataatat ttacaattta ctatttttat ctcaaaatag tttttggagg
     6241 aatttcattt agtgagattt tcttagttgt aggaattgta ctaattatct atcaaatatt
     6301 taagaaaaaa attaaagaaa agaaggtttt ttatagggtg ttaaaaataa taatcagcat
     6361 ttttttaata gtatttgtag ttactgagag tttaattatt ttttatccta aaaatagttt
     6421 agaatcaaaa agtgattatc tcttaatttt aggagcttct gtaaaaaaga ctacaccaag
     6481 tacaaccctg aagggtaggt tagacactgc attgaaatac ctaaaaatta atgataattg
     6541 ttatgtggta gtatctggtg gtaaaggaag tggagaaaaa ataactgagg caaaagctat
     6601 gaaagattat cttataaaaa atagtataga taaaaatagg ataatagaag aggataaatc
     6661 cacgaatact tatgagaatt ttaaatattc aaaattgaag atagaagaac atagtcaaag
     6721 aaaactaagt gatttaaaag taaaaatagt aacaacagat tttcatgttc tcagaagtaa
     6781 aatattagca tatagaaatg gatataaaaa tacgtctttt tatgctagta aatcaaaact
     6841 ttcatttgta ccaacttact atacaagaga attttttgca ttatggaaaa caatcatttt
     6901 tgatagataa taaataaaaa gactagatat aaactcatat ctagtctttt ctattccctt
     6961 aattctaatc cattttccaa ctttacttgt tgtagaaatt tcaacattgg gatttacaag
     7021 taattgctta taagaagttt tatattttac gattccaaaa tataattttc cttcataatc
     7081 cattataaat ccgaatgatc ttactcttgg tttattatct tcaactgtag caaaatagaa
     7141 tgttgagttt tcttttaaaa aattcattat tttttccatg attaaatcct ctt
//
LOCUS       DACTGW010000191         7182 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.191, whole genome shotgun sequence.
ACCESSION   DACTGW010000191 DACTGW010000000
VERSION     DACTGW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.191"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            24..1805
                     /locus_tag="I9573_002970"
     CDS             24..1805
                     /locus_tag="I9573_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HAT4797573.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(1887..2435)
                     /gene="lepB"
                     /locus_tag="I9573_002971"
     CDS             complement(1887..2435)
                     /gene="lepB"
                     /locus_tag="I9573_002971"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAT4797574.1"
                     /translation="MIRRKVMSVKKEIFDWIKSIAMAIVLAFVILQFIRPSVVSGESM
                     YPTLDDKDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIK
                     ISNSKVYVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVD
                     EDEILGKVMVRLLPLDNIGKVD"
     gene            complement(2457..3281)
                     /locus_tag="I9573_002972"
     CDS             complement(2457..3281)
                     /locus_tag="I9573_002972"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAT4797575.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILINEFIKLNF
                     SNTFINTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRVFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3538..4446
                     /locus_tag="I9573_002973"
     CDS             3538..4446
                     /locus_tag="I9573_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAT4797576.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            4590..6035
                     /locus_tag="I9573_002974"
     CDS             4590..6035
                     /locus_tag="I9573_002974"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAT4797577.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            6346..6798
                     /locus_tag="I9573_002975"
     CDS             6346..6798
                     /locus_tag="I9573_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAT4797578.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
BASE COUNT         2942 a          857 c         1054 g         2329 t
ORIGIN      
        1 tataacagaa ggaggtaatg taagtgagcc aatacataaa tataaattct cagttagacg
       61 ccctaaaatt gaaggagttc gagaaaatat tttacgaaaa tatcaatgag gacgttaaag
      121 tagtgccaaa agttactcct tttaaaggaa taaataccga tttgttgtat gttaaagatg
      181 gaagaatttt atttataaaa tttatggata caacagaaga tatatttttt attttagaag
      241 aagagttact tgaagttatg aatgaggaat atgaactttt gaaactaaaa atgggacaaa
      301 agaatagaaa tataagttat aactacgttt acattatgcc atacgttgaa gttgaggaaa
      361 catatgaatt tgaagaattt gttaataata atataataga taaaaataag cttcaagata
      421 taatgaataa gggttcgctg gaggagtatc ttaatgatga gaatgatgaa ataaacttaa
      481 atttattttt actagacata tgttcagaat actatataat aaatgataaa cttcatctaa
      541 atgaaaaatt taaaaaaatt tcattctaca atgatgacta taagtataca gcaactatga
      601 tggaagacgt tcaaattaaa gatgttgttt ctattaaata tgggaataca cttattgaag
      661 gtggttctgg tataggcaaa acagcaatta tgctttctag ggctataaaa ttagcaaaag
      721 tatatccaca tcataaatta gtcatattta cacatacaaa acaattgtgc aatgagcttc
      781 gagagaggat agagttatta tacaaagata ataacaattt agaagttcac acatttagtt
      841 cttttatatt taaactagct aaaaaattca atttaattgt agattataac atgttaaaaa
      901 atgattacga aaagaccttt aataatcttg taaaacaagc acagaacatt ataaaaaata
      961 aaaatatgtt caaggctatt tttattgatg aagcagaaag ctttttagag tatgaaatag
     1021 attttataag ggagttttta tataaaacaa aattcatatt taatgtgtgt tcttgtaact
     1081 ccttgaatat ctctaataga ttgaatatat ttaaaaaatt atataatggt atagaatttg
     1141 atgataagat tatcttaagt aaaaattata gacaagctaa agagattgta gattttacaa
     1201 ataaattttc aaataattct aactcatata tcaacgaatt aagaccaaat acagaattta
     1261 gtactttctt ttatactaaa gcacttagag gtggaaataa gtctgttgat ataataaaag
     1321 taagtgacct tgatgaccaa ataagttctg taatctggga aattgagtat ttaataagta
     1381 aaaaaggtct tgattattct gaagttgcta tcgtttatcc ttataataag aagaaattaa
     1441 aaagtggaaa aaccatttac tttcaatata tgttaaaaaa agcattggaa gaggcaaaaa
     1501 ttccttatat atgtgcagaa gataatttga caaatataag taagaaggta ggaactacaa
     1561 tagcaaatat atatgcaata aagaatttag agtataaagc ggtaatagta tgtgaattag
     1621 agatgttata caatcaaact ataaatgata ttgaacaaga ttatcaagta aatgactttg
     1681 taggagattt aaataaggtt tatttagcta tgagtagagc aacagactac ctaagtatag
     1741 taacaacttt taatgaagaa gcaagtgata taataagact tattacagaa tcaaaagata
     1801 tttagtaaaa aaatatttgt aatttaagaa aataaaaaga gactgagatt tgtaaaataa
     1861 actttaccaa ttcagtctct tattttttag tccactttcc caatattatc aagaggtaat
     1921 agtctcacca taaccttacc aagaatttca tcttcatcaa ccatacctac atcagggaat
     1981 ctactatcat tactattttc tctattatct cccattgcaa atagtttacc ttttggaaca
     2041 acagtatcta tatctccaga agtatagtta ttgtgtatat atggttcatt taataatttt
     2101 ccatttacat acactttaga atttgatatt tttattctgt caccttcagt agctataact
     2161 ctttttatta agtctttttt ctttcctgtt tctccatcaa ctaaattggt tttaaaaact
     2221 acaatatcgc ctttttcagg tttaccaacc ttgtatgata tcctatttaa aatcagataa
     2281 tctttatcat ctaaagtagg atacattgat tctccactta caacagaagg tcttataaat
     2341 tgtagaatta caaatgcaag tacaatagcc atagctattg acttaatcca atcaaatatt
     2401 tcttttttaa cactcataac ttttctcctt atcatttgtt attaaattag aatacattat
     2461 aatagaaaga aataagcaag aaccactata cccaagacta ttctataata accaaatact
     2521 ttgaagtcat gttttttgat atagtccata aaaaatttaa taactacaat tgaaacaaca
     2581 aatgctacta cagaaccaac acctaaaata agccattcaa atccagtaaa tccaaatcca
     2641 gcttttagaa gttttaatgc actagctcca agcattgtag gaatggctaa gaagaatgaa
     2701 aactctgctg ctacatatct agaagtacca agtaatactg cacctattat agtagaacca
     2761 gacctagatg tacctggtat gagagctaga cactggaata atcctataca aagagctaat
     2821 ttatatgtaa cttgagaaaa atctttatac ttaggtcttt tatttctctt ttcaagccct
     2881 atcattatta taccatagac aataagagca atagcaacta ctgttgaatt aaagaatact
     2941 ctgtctatgt catcttcaaa cagtattcca ataactccag atggtataac tgcaattata
     3001 accttaagcc ataaacgaac tgtttctttt ttctgtttaa tattctttgc actatcaaaa
     3061 ggatttaact ttctaaaaaa tataacaagt acagctagta ttgaaccaaa ttgaattaca
     3121 actaaaaatg tatttataaa tgtatttgaa aaattcaatt ttataaactc attaattaat
     3181 atcatatgcc ctgtactact tactggtagc cattctgtta ttccctgaac tatgcccaaa
     3241 atgactgcct taaaaatttc aagcaaacta aatgtttcca aatcaaaata cctccaatac
     3301 aaatttatag tagcatattt tattcataga ttaataaaaa aatgccacct tttactaact
     3361 tataatacca tagtactgtg tagtgcacaa tggaagattg atattttcat aaaaccttca
     3421 taatataaca aaataaactt aaaagttatc tattgaataa ggggtatatt tgagggggaa
     3481 atatatggta tagtaaaagt atagctattt tattttatga aaaggaaagg agttattatg
     3541 tatagtacaa aaatactaga tgattatgag tatgaaactt ttaatgtttt ttctacaaaa
     3601 ttaaatccaa acacgaaaca cgattattta tcaaaggtta tactgtttaa agaattttta
     3661 aaagggaaag aattgattta tgctacaaaa gaagattgta aaaattttgt agactatata
     3721 cagactaaat atgcgaaatc aacttgtgaa aagatatata gttatttaca tagtttttat
     3781 aacttcttaa aaaaagaagg atacatagat ataaatccat ttagatatgt agaaaaaccc
     3841 acagtaacaa gaataaaaac aaaagatgat gttctaagta tacaagaaat aaataaactt
     3901 ataggtatcc taccaaaatt aaatataaga gatagagtaa taatagtctg tctagttact
     3961 actgggtgtt tattaaatga attagttagc ttaaagtgga aagaccttat ggtagatgaa
     4021 aacgataact catatgtaag gttaggtaag ggaagaaaag aaagagtagt taaattacat
     4081 ccatactttt ttaaactttt agaagattac aggaattatt ctgggcttcc agaggtaata
     4141 atacctagtg atgattttat ttttacaact caaaagagta attctataac agatagaaat
     4201 gtcagactta tagtcaaaaa agcattagat ttagctggac tttctcaata ttcagcaaga
     4261 gattttagac attcatttgc agcaataagt ttaaggcttg gggcagatga aagtgatgta
     4321 aaaaatcagc ttggatggag tgataaatac tatgcaatta gatataagta tgtattaaat
     4381 tttgttgata gtgaaatagt ggattactta attgaaaaag ataatttgga tataaataaa
     4441 aaatagtaat aaataaaaaa tactatatat agatgtgaac aaaaaataaa aaagtcttgt
     4501 tatagttcgt aaaattatat ataattattt atgtactaat gtgtacacag taatcacacg
     4561 acaaaagatt taaggaaggt gctaatataa tgatagataa taaatatact acttactcaa
     4621 acccacttac agacagatat tgtagcaagg acatgagtta tatattttct ccacaattta
     4681 aattctctac atggagaagg ttatgggttg cacttgcaga aggtgaacat gagttaggaa
     4741 tcaatataac tgaagaacaa ttagatgagc taagagctaa tatagataat ataaattttg
     4801 atgaagctag aaaaatagaa aaagaagtaa gacatgatgt aatgtctcac gtaaaagctt
     4861 atggtttaca atgtgaaaaa gccaaaggaa taatacattt aggagcaaca tctgcatatg
     4921 ttggagataa tacagatgtt atacaaatga atgaagcttt aaaacttcta agagtaaagc
     4981 ttgtaaattt aataaacaat ttaaaagaat ttgcacttaa gtacaaagat atgccagctc
     5041 ttggatttac acacttccaa gctgctcaac ttacaacagt aggaaaaaga gcttgtttat
     5101 gggctcaaga tttaattctt gatttggaag atttaaacta tagaatagat aacatgaagt
     5161 taagaggagt taaaggtact actggtactc aagcaagttt tttaagctta tttgaaggag
     5221 accacgaaaa agtaaaagag ttagataaga aaatttgtaa aaagatggga taccattctt
     5281 cctatgcagt aagtggacag acatatacta gaaagttaga ttatcaagta ataagcatac
     5341 tatcagggat agctcaaagt atgcataaga tgactaacga tataagactt ttacaaagtt
     5401 taaaagaatt agaagagcca tttgaaaaaa atcaaatagg ttcatcagca atggcttata
     5461 agagaaaccc aatgagaagt gagagaatag catctttatc taagtatata atctctgaat
     5521 ctataagccc tgcaatggta caagccactc aatggttaga gagaacatta gatgactcag
     5581 ccaataagag attggcaata cctcaagcat ttatggcagc agatgctata cttgaaatag
     5641 ggataaatgt aactgatggt ttagtagtat atgaaaatat gataaataaa cgtgtaaatg
     5701 aagaacttcc ttttatggct acagaaacaa tacttatgga agctgtaaaa agaggtggag
     5761 atagacagga attacatgaa ataataagag aatattctat gaaagctgcc tatagagtaa
     5821 agcacgaagg taaggataat aacttaattg aacttataat gaatgatgat tcatttaaaa
     5881 tgagcaagga agaaatacta tctataatgg acccaaaaaa ctttataggt agagctccag
     5941 aacaagttgt agaatttgta aatgaagttg tagaacctgc tataaaagat tataaggaag
     6001 acttaggaaa agtagatgtg gacctaagag tatagaattg tacagataaa atataattaa
     6061 aaacggctat cttatttata aataagatag ccattttaat gggctagcgt cttctcttca
     6121 gcgctgtgaa gagttcttct ttacttacta taccatgaat ttaataaaat agctatataa
     6181 ataatattta atagaaataa tataaataaa aataaaaaaa tgataagatt actaattaaa
     6241 tttaaacatt cagcaaaaac tattgtactt gcaatatttt taatgtaaac tgaaataaag
     6301 ttatttagat aatttaataa attttaaaac aaccgaggtg attatatgaa ctatatagta
     6361 aaagatacca taagtttttt agtaaaaaca tttcctaaga tttattctag tctgtactta
     6421 gaagacctaa aaaaatttgc accagattat aatgtaaata aaacacaatt aagagcatta
     6481 gttttcataa aaaattatgg agtaataagt atgactgatt tatgctctaa gttaaatata
     6541 gaaaaaggca gtttaactag tatggttgat gatttaactg ataagaagta tgtaacaagg
     6601 aaaagagatt tagttgatag aagaaaatat ctaattgaca ttactgaaga gggagataag
     6661 atagcaactg attttatgga taaattaagt gatggtttag gagaaaaact ttctaaatta
     6721 actgaagaag acagaaaaaa atatttagaa gcaataaata ctcttcaata tatattaaac
     6781 aaagaagaat ttagatagaa aaaatataaa aaatttttat atttaatagt actaagatta
     6841 attctttgtt attcatttaa ctatataatt ataagtctaa atacaatcta aaaagtacat
     6901 cttaaatttt cttgatgtac ttttttataa tttcttatac attttcttat ttgttagtac
     6961 ctactcatac ctctttgtgt aatttatatt tttccatttt atattcaata attttatttt
     7021 ctaatctctt tttaaatttc tatatatcac aatgttacta tgtctttgtt tgaaaaaatt
     7081 tacagttttg aaaaaattac tatttatgta aaaatatttt aaaacaaatt ataaagtttc
     7141 aaaaacaaaa taaaaatata taattatgac atagaaaacg tt
//
LOCUS       DACTGW010000192         6999 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.192, whole genome shotgun sequence.
ACCESSION   DACTGW010000192 DACTGW010000000
VERSION     DACTGW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.192"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            6..82
                     /locus_tag="I9573_002976"
     tRNA            6..82
                     /locus_tag="I9573_002976"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:His,seq:gtg)
     gene            91..166
                     /locus_tag="I9573_002977"
     tRNA            91..166
                     /locus_tag="I9573_002977"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Lys,seq:ttt)
     gene            174..247
                     /locus_tag="I9573_002978"
     tRNA            174..247
                     /locus_tag="I9573_002978"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Cys,seq:gca)
     gene            255..331
                     /locus_tag="I9573_002979"
     tRNA            255..331
                     /locus_tag="I9573_002979"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:289..291,aa:Arg,seq:acg)
     gene            344..419
                     /locus_tag="I9573_002980"
     tRNA            344..419
                     /locus_tag="I9573_002980"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Val,seq:tac)
     gene            495..908
                     /locus_tag="I9573_002981"
     CDS             495..908
                     /locus_tag="I9573_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797579.1"
                     /translation="MYIVIGNEIVDSEELKIIIDENSKFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLSSLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            1217..4648
                     /locus_tag="I9573_002982"
     CDS             1217..4648
                     /locus_tag="I9573_002982"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAT4797580.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            4890..6818
                     /locus_tag="I9573_002983"
     CDS             4890..6818
                     /locus_tag="I9573_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAT4797581.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         2895 a          776 c         1331 g         1997 t
ORIGIN      
        1 ttatggtggg tatagctcag ttggttagag cgccagattg tggctctgga ggtcgtgagt
       61 tcgactctca ttatccaccc caacttatat ggtctattag ctcagtcggt agagcacctg
      121 acttttaatc agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa ggcggcgaca
      181 tagccaagtg gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa tctgggtgtc
      241 gcctccaata aaatgcgctc atagctcaac tggatagagt gtctgactac gaatcagaag
      301 gttaggggtt cgagtccctt tgggcgcacc atatgaatag cgagggatta tagctcagct
      361 gggagagcac ctgccttaca agcagggggt cacaggttcg agccctgtta atcccaccag
      421 tgcaaacctc tagtaaaact actagaggtt ttatttttga ttgaaaaagt ttttatattt
      481 aggaggtaag aaatttgtat atagtaatag gaaatgaaat agttgatagt gaagaactta
      541 aaataattat agatgaaaat agtaaattta aagttgaaaa agatttaagt aaaagcacaa
      601 aaagagaaga tgtaatagca tatcagttaa gtatagattt aaattaccta gattctctta
      661 taaatgagca atgtaatttg tccagtttat ctgatgaaga gaaatttgat gaatatatga
      721 ctttgtctga tgaacttgca ctggatttag aagagttaat gccaaaatat acaataataa
      781 atgctagagc atataaatta gacgaggtag atggaattgt aaaaataata ctagctgtag
      841 cgtatgcaga tttagggcac ttaaaacttt ctgatgtagt aaagaggcta tctagacaag
      901 tagattagga tatgaatatg aactatatta aaaaaatgta taaaatctta cgtaaagatg
      961 aaaataatta tgtaaacata gaaaaaaaca gtataaaatc aagtcaattg tagaaatttt
     1021 cacataaatt catctgaaat tctataaaaa atgccttaat tttaatgagg taaatcgagt
     1081 ttacacagtc taaaaattac ttatatttat aaatttatct tattaattaa ggaactttct
     1141 taaacatctt caaaaataaa gtattttagt ctataatctt taatattaca caaacaataa
     1201 aggagaagaa tagagtatgc ttaaaaagtt taataaaata ctagtggcta atagaggcga
     1261 aatagctata agaatattta gagcctgttc agaattgggg ataaaaagtg ttggtatata
     1321 cagtaaggaa gataagtatg gattgtttag aacaaaagca gatgaatcat atttgatagg
     1381 agaaggtaaa gggccaatag atgcatattt agatatggat ggaataatag acctagcaaa
     1441 gagaaaaaaa gttgatgcta tacatccagg atatggtttt ctagcggaga atgcagaatt
     1501 tgctcgtaaa tgtgaagaga atggaattac ttttatagga ccatcatcta aggtaatgaa
     1561 tatgatgggg gataagatta actctaaaaa aatcgcaaaa gaagttaatg ttcaaactat
     1621 accaggagta gaaaaagcaa taagaagtac tgaagaagca aaggaagttg caaacaaaat
     1681 aggatatcct gttatgataa aagcatctaa tggcggtggc ggtagaggta tgagaattgt
     1741 acatcgcgaa gaagacttag aacttgaata tgaaactgct tgtagtgaat caagaaaagc
     1801 ttttggagaa gatattatat ttatagaaaa atatatagct gatccaaaac atatagaagt
     1861 tcaaatatta ggtgataatt atggaaatat agttcactta tatgaaagag attgttcagt
     1921 tcaaagaaga catcaaaaaa taatagaata tgcaccagct ttttcgttag atgataaagt
     1981 aagaaaagag atatgtgaag atgcagttaa gttatcaaaa catgtgggtt attctaatgc
     2041 gggaacatta gaatttttag tagatgcaaa tggtgggcat tattttatag agatgaacac
     2101 aagagtacag gttgaacata ctgtaacaga gatggttaca ggaatagata tagttcaaag
     2161 tcaaatacta atagcccaag gttacagctt agatagtgaa gaaattaata taaaatcaca
     2221 agatgatgtg gaaataagag gttactctat ccaatgtaga ataactacag aagaccctaa
     2281 gaataaattc atgcctgata caggtaaaat tcaagtttat agaactggtt caggatttgg
     2341 tataagacta gatggaggaa atggatttac aggggcaaac ataagtcctc attatgatag
     2401 tttattggtt aagacaatat cttgggatag aacattccaa ggagcaataa ataaaactat
     2461 aagatctata aaagaactta gagttagagg tgttaaaact aatgtaggat tcctagttaa
     2521 tgttttaaat aatcctatat tctcaaatgg taaatgtagt actaagttta tagatgaaaa
     2581 tccagattta tttgaaataa cagaaagtaa agacagagga acaaaattac ttcaatttat
     2641 aggagatgta attgtaaatg ataatgcttg taaagaaaaa ccactatttg atgcattaca
     2701 tgacccaaga atggataagg atggttcaaa atcagaagga agtaaaatac ttttcgataa
     2761 gttaggtaaa tctgcatata tagaaaagat aaaaaatgat aagaaattac tattaacaga
     2821 tacaacaatg agagatgcac atcaatcatt attagcaaca agaataagaa catatgactt
     2881 attaaaagct gctaagccaa cagaaaaata tcaaaaggat ttattctcac ttgaaatgtg
     2941 gggaggagct acttatgatg ttgcatatag atttttaaaa gaatctcctt ggcgtagatt
     3001 acaaaagcta agagaagaaa taccaagcat aatgttccaa atgttgctta gagcatcaaa
     3061 tggagttgga tataaaaact atccagataa tgtaatagaa gaatttacaa aagaatctgc
     3121 tagacaaggt atagatgtat ttagaatatt tgattcatta aactgggttg aaaatatgaa
     3181 accatctatt aatacagctt tagaaactgg aaaaattgtt gaagcaacta tgtgttatac
     3241 aggtgatata ctagataaga ctaaaactaa atacaatcta gaatattata ttaaaatggc
     3301 acaagaatta gaaagcttgg gagcagatat aatagcaata aaagatatgt caggtcttct
     3361 taaaccatat tcagcttaca cactagttaa agagttaaag aaaaatgtaa aagcaccaat
     3421 acatttacac actcatgata caagtggaaa tggtgtagca acttgtttaa tggcgtcaga
     3481 agctggagta gacataattg atgctgcatt agaatctatg gcaggactta caagtcagcc
     3541 atcgcttaat gctatagtag aggcacttaa gaatacagag agagatacag gaatagattt
     3601 atttggatat gatgagttag gaaaatatta taaggattta agaaaagttt ataataaatt
     3661 tgaaagtgat ttaactaatt cttgtgcaga aatatataat tttgaaatac caggaggcca
     3721 atatacaaac ttaaaacctc aagctgatag tttaggatta gttaatagat ttgatgaagt
     3781 taaagaaaaa tataaagaag ctaatgaagt tgtaggagat ataataaaag taactccatc
     3841 ttcaaaagtt gtaggagact tagctatctt tatgactaaa aataaattag ataaagacaa
     3901 tataatagaa gaaggtaaaa atttatcatt cccagattct gttgtggatt actgtaaagg
     3961 tatgattggt caaccagaag gtggaatacc aaaagattta caagaggtag ttctaaaagg
     4021 tgaagaagct ataacagtaa gaccaggttc attattacca gcagaagatt ttgatgaaat
     4081 agctaaatat ctaaatgaaa agtatgatat aaatgctaat ataagaaatg ttataagtta
     4141 tgctttatat ccaaaagtat atgaagatta tatcaaacat ctacaacatt ataatgatat
     4201 atctaaactt gaaagtgatg tattcttcta tggattaaat aaaaatgaag aatgtgaagt
     4261 agaaatagaa gaaggtaagg tattaacaat aagattagtt gaaattggtg aagttaagga
     4321 aaatggattt agaactatag gatttgagtt aaatggaatg gttagagaag tagaaataaa
     4381 agataagaat ttctctggaa aaattaataa tgttgaaaaa gctgatatga atgaccctct
     4441 tcaaattgga gcaagcatac caggaaaagt tataaaaata atggttaaag aagaagatga
     4501 agtaaaagca aatcaaccat taatcgttat agaggctatg aagatggaaa ctataatagt
     4561 tgctaagact gatggagtaa ttaagtcaat caaagtaaaa gaagatgata tggttgaaga
     4621 taaacaatta ttaatgataa tgaagtaatt taattaaaat aaattaaaaa gcactctgaa
     4681 atgtttaata aaaaatgttt tagagtgttt ttatttattt taattaaata tttgttaaaa
     4741 aaaagaggac aattacctcc agatgtagaa ataaagctag taaggaaaat gttatctaaa
     4801 aattctaaat atagtaaata ttttccataa tatttcttag gaaataacag caaagataaa
     4861 atacataaat ttaaaaggtg ggtgcttata tgaaggttta tgatagtaaa atgttgagaa
     4921 atgtagcagt attaggacat agcggatgtg gaaaaacaaa tctaatcgaa actatagctt
     4981 acacagcaaa cactaataag atacctaaat taacagacaa ggtaaatatg acttatagca
     5041 tgggattaat acctatagag tataatgatt ataagtttaa cttattggat accccaggat
     5101 attttgactt tagcggagac gttgtgtcat ctcttagagc aagtgatgca gcaataatag
     5161 taatagatgc aacagcgcct attcaagttg gtacagagaa atcattagaa ctaactgaga
     5221 gtattcctaa gataatgttt ataaataaaa tagataatga aaaggcaaga tacaaagatg
     5281 caatagcaat gttaagagaa aaatacaata ataagatagt tcctatgata agtcctatat
     5341 acaaagataa aaactttgta aagttacata atgtttttga aaacatagat gatttagaag
     5401 gtgaatttaa ggaacaagct atgagtgtta aagaagcatt aatggaactt atagcagaaa
     5461 ctgatgacca aatattagat aaatatttta atggagaaga actaactacg gaagagatac
     5521 aaaaaggtat tattataggt atacaaagag gtgatattat acctgtaata tgtggttcaa
     5581 ctataaataa tataggcaca aaggaaatat tagatacaat atcaagttat ttagaaccta
     5641 tctttacaga ggaaagcaaa ccatttaggg gattagtatt taaaactatg gttgacccat
     5701 ttgttggaaa gatgtcatat ataaagatta ctgagggtgt actttctaag gataaagatg
     5761 tttttaatat aaataaaaat gtaaaagaaa aaatagctaa tatatataca ttgagaaata
     5821 gtgaattagt agaaatagaa aaagctaaag ctggtgatat agtagttata actaaagtta
     5881 attctctaaa aacaggagac actatatctg cagataaaga tgcagaagct ttagaaaaga
     5941 tagattttcc taaaccacaa atatattatg cagtaactcc taagaataaa ggtgatgaag
     6001 aaaaagtagc atctgtatta aataaactag tagaagagga ccctacatta cattggtata
     6061 gaaatacaga gacaaaacaa gcacttttag gaggtcaagg agaacttcat ataaaaacaa
     6121 ttaaaaataa aatgaaagat aagtttggtg ttgatgttga attgaatgac cttaaagttc
     6181 cttatagaga aactataaaa ggaacagccg atgtacaagg aaaacataaa aagcaatcag
     6241 gtggtcatgg tcaatatgga gacgtaaaaa taagatttga aagatgtgaa tctgattttg
     6301 aatttacaga agaaatattt ggtggttctg taccaaaaca atatatacca gcagttgaaa
     6361 aaggtctaaa agactctatg caaaaaggta tattagcagg ttatcctgtt acaaacataa
     6421 aagctacgct ttatgatggt tcataccatg atgtagactc ttctgaaatg gcctttaaga
     6481 tggcagcaag tgcagccttt aaaaaaggta tggaagaagc gcacccaata ctactagaac
     6541 ctataatgaa acttaagata actgttccag aagaatatat gggagatgtc atgggtgata
     6601 taaataagag aagaggaaaa atatttggaa tggaaccaga tgacaaaggg aaacaaataa
     6661 tatttgcaga agctccacaa gcagagacat ttaaatatgc aatagattta agagcaatga
     6721 cacaaggtag aggatacttt gaaatggaat tagagagata tggagaggtt ccatcacagt
     6781 ttgcagaaaa aataattggg ttagctactg caaagtaatg aacaaaataa atagaaaaat
     6841 aattaatata tattattaaa aatattttta gggttttaac taggtatagg tttatgttag
     6901 gtaaacttat acctagtttt tatacatttt tgatgaataa aaaaatatat aatactttct
     6961 taaatgtttt aataaaaaga aagtattttt ttattatta
//
LOCUS       DACTGW010000193         6712 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.193, whole genome shotgun sequence.
ACCESSION   DACTGW010000193 DACTGW010000000
VERSION     DACTGW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.193"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            36..2081
                     /locus_tag="I9573_002984"
     CDS             36..2081
                     /locus_tag="I9573_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAT4797582.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNILIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILNSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            2342..3541
                     /locus_tag="I9573_002985"
     CDS             2342..3541
                     /locus_tag="I9573_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT4797583.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDICRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            3593..4288
                     /locus_tag="I9573_002986"
     CDS             3593..4288
                     /locus_tag="I9573_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HAT4797584.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            4301..4978
                     /locus_tag="I9573_002987"
     CDS             4301..4978
                     /locus_tag="I9573_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797585.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            5089..6690
                     /locus_tag="I9573_002988"
     CDS             5089..6690
                     /locus_tag="I9573_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAT4797586.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDDLRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDNKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGIIKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
BASE COUNT         2681 a          668 c         1281 g         2082 t
ORIGIN      
        1 ttactttcta tactcgttaa acaggaggtg ggactatgaa aaaacataat attttatttg
       61 tatcaactga tgacaaaata aatatagata tatcaaaaca attggaaaat atatttggag
      121 aattttgtaa tatagataac ttggtatatg taaataggat aaatattgaa ctgtcaagct
      181 atgagcttgt agtatgttca gacaatgata taaaggaata tatacataat aatattgata
      241 aaaatatact tattgtaata gtacatcgta ctataaatat tgaaaatata aatcagataa
      301 taagtataga aaatgattct gatgttatgg ttatagatgc ttataaagag tcagcagatg
      361 aaactgccaa gataattaga aaactaggat taattcatat taatctaata ccatattatc
      421 caggttgtga taagtctaaa tgtgagattg gaattattac gggaagcaga aacagcatac
      481 cacaaaacat aaagcaaata attgacatag gtgataaggt aattgatata aatacagtta
      541 tagaaatctt tacaaaacta aatatatcta tagataaatt acatattatt aaggaaaaat
      601 acaatgaaga tacagtaagt ggatataggt attatacaac tatgaataaa accatgaaga
      661 gttttttgga gattatagat gaaggtattg cctctgttga taaattagga aaatttatat
      721 attgcaacaa agtattttct aatctagttg gaatagacca aaatgaaatt atatctaata
      781 actttatgga cttatttagt gataaggttg tcaaaaaaat attttttcaa gaagatgaag
      841 ttaatgatga agtagtaaat ttaaataaca aaaaactgat aattaataaa gtaaatgttt
      901 atgaaaataa tgaacgaata aagagtatta taagcattaa aaatataagt gcaattcaag
      961 tgcttgagga caagattcaa aataaacttc agtcaaaagg ttttgtatca aagtatacat
     1021 ttgagagtgt tgtaggtgag agtaaaataa taaaagaaaa aattaatatt gcaagaaaaa
     1081 tagcaactac agacttttct gtgttgatac ttggagaaaa tggaacagga aaagaaatat
     1141 ttgcccaggc aatacataat gaatctctaa gaaaaaacaa gccatttgtt gcagttaatc
     1201 tatcttcttt gtcagataca cttattgaaa gtgaactttt tggctatgat gaaggaagtt
     1261 ttacaggagc aataaaaggt ggaaaaatgg gtatatttga aagagcgcat acaggaacaa
     1321 tttttttaga tgagataggt gatatatctt tagatgtaca gcagagattg ctaagagtat
     1381 tacaggaaaa agaagttatg agaatgggcg gaagtaaaat tataccaata gatgttagaa
     1441 ttatagctgc aacaaataaa gatttaaaga aaaaaattaa agaaggttct tttagagaag
     1501 atttgtatta taggattaat gtattacata tagagatacc tagacttaga gaacgtaagg
     1561 aagatatacc tcttataagt aagtattttt tagatgaaat aaatagcaac aaatgtttta
     1621 cagaggaatc aatgcaagct ttaacattgt atgaatggcc aggaaatgta agagagctta
     1681 aaaatttaat ttattatatt gatactatag ttgaggaaga tagagtagat tacgagcatt
     1741 tgccagaaca atttagattt gaaaaaaaca atactttagt taatgaaaat ttcgattcaa
     1801 ttatcttaga tttcaaacaa tctaatttct ttgaggaaag tatatgtata ttgaacagta
     1861 tagaaacttg gaataataaa aatatattat taggaagaaa taaactgcaa gagattttaa
     1921 aagaaaaaga tatagtttta agtgtagacc aaattagaaa aagaatagat aagttaaaga
     1981 gccatggttt acttttatct ggagtaaaaa aacaaggtag ttttataact gatgaaggaa
     2041 agaactttat atcatatgta aaaattaagg gagtgatata ggggactaaa aaggcaaaac
     2101 accctaaaaa aatccctata atggatagaa ataaataaaa ttatctttaa gattaagata
     2161 attcaaaaat attgaataaa taatctaaga agattatttt taataaaaca gtagtaaata
     2221 tcaaccttac tactgttttt attttttgca agatttatta tacaattaaa atttatattt
     2281 tggcatatca attgctacat aggaaaaata taaaaaattg tagaacttgg aggatgttgt
     2341 tatgaagtca gcttacaaag gatatgaagc ctatagttat ttagaaaaag gaaaagatat
     2401 ttcatctatt gagatgtgta aaggagataa aagagttaaa gagtacctta ttgaattaga
     2461 tgatattcaa gaagctaagg ttaaaagtat agtggatgaa aaaatattta tatcattaca
     2521 tgaacatcca gtattatttc cagaacacct agaaagagat atatttgaat ataataggga
     2581 aggtaaacaa agatgcgctt atgaagcatt atcaagagga tattatgatg ctatatttga
     2641 taatttaatg gatggtgtat gtacgataac atcaaattca gggtggaaat gggatgatat
     2701 attaatagat ttaggcatga gactatgtga tttagctcat caagattttg tcataaagtg
     2761 tgaaaaagta gaagatatat gcagagctaa aagtgaaggt aaagtagcat tgataccaac
     2821 attagaaggt gcagcaccaa tagaaaatga acttgataga atagatatac tgtatggatt
     2881 tggtataagg ttaatgggta taacatacag cgaatccaat gcattaggtt caggactaaa
     2941 agaagagaaa gatggaggtc ttacaaattt cggaaaaaaa gctgttgaaa gaatgaataa
     3001 aattggaatg gcaatagatt gctctcacgt tggagttcaa actacacttg atgtaataga
     3061 atatagcaag gacccaataa tattaagcca tgttggggca agaaatttat ggaacacaaa
     3121 gagactaact ccagatgaag tattaatagc atgtgctaaa aaaggagggg ttataggtgt
     3181 agaagcagca cctcatacta caataacaga gaagaataaa gaacattcaa tagagtcatt
     3241 tatggaacat tttgagtata ttaaaaattt agtaggaata gaccatgtag cttttggacc
     3301 agatacagta tatggcgacc atgtaaaatt acactcagta ctagctgcta aatttgatat
     3361 aaaaaaaata acaggtacaa aagaacatcc caaagtagaa tatgtaaaag gtcttgagaa
     3421 cccaacagaa acttcaaaaa acattgtcag atatttagta aagcatggat atagtgatga
     3481 ggaaatagag aaggtactag gtggaaatat acttagagta ctaaaacagg tttggaaata
     3541 gatatattag aaatattaat aaaatttgag gatttatagg gaggttttat aaatggatta
     3601 tttaaaatta gctaatcatc cacttttgtg gatagcgagt acagtagcag tatcaatagt
     3661 agtggtacaa tcccttatat ttgctataaa atcttgcaaa gttggtaaaa caatgggaat
     3721 aacagataaa caaataaagt cagctgttaa gagtagtgct atttcagcta ttgggccttc
     3781 aatgactata tttgctggaa tggtatcgct tatagtaact atgggtgggc ctatagcatg
     3841 gatgagatta tcatttatag gttctgtaat ttttgaatca atgtcagctg gatttggaac
     3901 agatgcatta ggtataactt taggttctcc tgaaatgaca aaacttgcat ttacaaatgc
     3961 agtttggact atgatattag gttctttagg atggataata ttcacattac tatttggaca
     4021 taaactagat aagataacca atgttatatc tagtggcaag aaatcattta taccaataat
     4081 atctctagga gcaatgttgg gttcatttgc atatctaaat gctgataggg tgttgcgttt
     4141 tgataatgga acaattgcat gtataagtgg aatggctata atggtaatat tgtgtatgct
     4201 tgaaaagaag aaaaatataa aatggttaag agagtggggg cttacaatat caatgtttag
     4261 cggaatgata atagcttcag taatataagg ggggagtttt atgagtaaag taaaatctat
     4321 atacaatgaa gaatatttac cttttatgat aaggtatgga agactaacac tatctttagg
     4381 tattatagca gcattagttc ctggaataat tttgagcttt ggatttggta ttatgcctcc
     4441 aatatcagca ttattagcaa gtacaatggc aattgtcagt atgagtgctc caaattacat
     4501 aatagaacca gtttcttatt caccaatctt aggtatccca ggaacttata tgagttttct
     4561 ttctggtaat atatctaata tgaggctacc atgttcaata gcagctcaaa aggcagcaga
     4621 agttgaatca ggtacagaag aaggctcgat aatatctaca ataggaatag ctgtatctat
     4681 tttagtgaat ataagtatac taactatagg ggtaatttta ggaggaagcg tattatctaa
     4741 aattccagct gaagtagtag aaaaattaaa cttaatttta ccagctctat ttgggtcagt
     4801 ttttggacaa gtatttttac aagataaaaa attaggtcta gtagcaattg taatatctgt
     4861 attgactatt atattatcaa agcaaggtat aataccacag tctctagtag ttcttatatg
     4921 tgtatttggt actatattaa ttgcaagagc tatgtataaa gataagctta gtgactaatt
     4981 acaatgaaat aatagtgaat tttcaaatat gaaatataat aatttgattt tcaaatataa
     5041 aaattacatt aagattattt aaaaattggt ttttaaggag ggaatatttt gatatatgat
     5101 ttaatcttaa aaaatggatt tataatagat ggtacaggta atcctggttt ttatggagat
     5161 atagcaatta aagacaactt aatagcaaaa atagattcta agataaatag caatacaaat
     5221 aaagagattg attgttgtgg aaaagttata acacctggat ttattgaccc tcatgttcat
     5281 gaagaaatag tagcaatact agatggtaaa tttgaaaagt ttctaaaaca aggagttaca
     5341 acaactataa atggtaattg tggacattct ataacaccat attcttctga aaatgtatat
     5401 gaatatatgt ataaaaatgg tctattactt gaagaagaaa aaaagtatct tatagataaa
     5461 aataaatgtt ggaataattt tacagaatat tgtgacctta tatctaaaag tggtatttct
     5521 gtcaatatgg gattcttgtt ggggcatggg actataagat ggagtgttat gggaggttct
     5581 aaggatagac ctcctacaga aaaggaaaaa aatgaaatta cagatataat aaatgatgga
     5641 atgaaaagtg gtgcttttgg aatatctact ggattggcat atataccaag taagtatgct
     5701 gatattgatg aacttgttga tattgcaagg cagattaaag agcatgatgg tatatataca
     5761 tctcatatta gagattacat aggaagatat aatgcagtaa aagaagccat agaggttggg
     5821 caaaaaagtg gtgctagggt tcaagtatct cacttaagtc cagttgaaat agaagcattt
     5881 gatgagatat taaaggctag gtataatggt gttgaaataa tggttgacac agtaccaaga
     5941 agttctgggc attgtatgaa gaaaaaaaga gtaattcaat ttattatggc aatatcatct
     6001 agtctatttg aattaggtat agatggggta atggatgatc ttagaaatga agagggaaga
     6061 acacttatat taaaagaagc atttatactt ggagataggg ggagtataat acttctaaac
     6121 actaaagata ttaatatgga aaagaaatca atacgtgaaa tagctacaca aaaaggaata
     6181 gatgaggaca agttattact cgatttactt ttagatggag acgaagaact tatcttttgt
     6241 ttaggaggaa tgtatagagc tgatttccca gataaactac atgataataa aataattgat
     6301 aatccatttg ttatggttgg ttcagattgt ttgtttagtg tgggtgggga tatgtcatgg
     6361 tttgaactac aaagaagagg agcttttcca attttcttca atatgtacag aaagtctggt
     6421 gttagattgg aagagattgt aagaagagtt acttcattac cagctaggca atttaaaata
     6481 aaaaatagag gtattattaa agaagggttg atagctgata tagcagttat tgacatggat
     6541 aactatagtt attctaggtc agaagatatt gatttttcaa agccacaatt actagccaat
     6601 ggtgttgaat atgtaattgt aaatggaaaa attgcgttag aaaatggaga gattactgag
     6661 aataaatgtg gtgaagtatt aaaaagataa ggttttaaaa ataataggat tt
//
LOCUS       DACTGW010000194         6691 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.194, whole genome shotgun sequence.
ACCESSION   DACTGW010000194 DACTGW010000000
VERSION     DACTGW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.194"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(105..713)
                     /locus_tag="I9573_002989"
     CDS             complement(105..713)
                     /locus_tag="I9573_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAT4797587.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(822..3560)
                     /locus_tag="I9573_002990"
     CDS             complement(822..3560)
                     /locus_tag="I9573_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HAT4797588.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNRKFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3708..4499)
                     /locus_tag="I9573_002991"
     CDS             complement(3708..4499)
                     /locus_tag="I9573_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAT4797589.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4535..5191)
                     /locus_tag="I9573_002992"
     CDS             complement(4535..5191)
                     /locus_tag="I9573_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797590.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5184..6149)
                     /locus_tag="I9573_002993"
     CDS             complement(5184..6149)
                     /locus_tag="I9573_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HAT4797591.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEHVKQYLTSKGIRWEEMINE"
     regulatory      complement(6255..6361)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         2141 a         1120 c          605 g         2825 t
ORIGIN      
        1 aattctataa agttattttt atcaaactaa aaattctata aaaaaataag atacatacct
       61 ataaaatacc ttcctataga tacatatctt acctatataa aaacttattc tatataaaaa
      121 cgccataaaa aatcttttgc ttcttctgca taatctatat ttatacgttt actagttttt
      181 atatctaact catctttact aaatactttt tttcctttat catcataata aaaatcactt
      241 atatataatt cttcacccat tatactctta ctatttagac ttctatctat ttttaaagct
      301 ttgcaaagtt ttcctggtcc gttagtaata ttttttactt gatatttact caactcagtg
      361 taagctttgt tgtatctatt catagatatc tcatctaaag aagttatagg ctctacccct
      421 cttattaata cacactctgg cacattttct atatttgcag ataaatttaa gcaattatac
      481 ataccataaa ttaaataaac atatatatgt ccaccatcta aatataatgg cttagttcta
      541 tctgtctttt tattgccata tacatgtgct cctttatcat ttacacccat gtatgcttct
      601 gtctctatga ttttggtaac aattacttta ttttcatatt tccttattag atatttacct
      661 aatattgatt tggctaaatc aattccattt tttctaaaaa aatccttttc caaaatttca
      721 cctcataaaa tttatttaaa acgcatcaaa tacaactaaa gtaataaata aaattaactc
      781 ttattaatgt gatatatata aacaataaat ttagccattt attatttata tatatcaaaa
      841 aattctatct caaacttttc aatattatca ttgtaatttg aattatcata tacttcttca
      901 acaacctctt taattgggaa tataactttt acattagttc cttctcctac cttactatct
      961 atgtaaatct tcccattttg aatttcaatc aatgattttg taaggcataa acctatacca
     1021 cttccaaatt catttgatat accactgttt tcaatttgtt taaatcgatt aaatatatca
     1081 tttaattttt ctttgtctat gcccactcca gtatctttta taattatctc tactgtttcg
     1141 tcttttttat atatacctac aaggatttct cctccttcta aagtaaattt tatagcattt
     1201 gataatatat ttagtattat tctttctata gattctgggt caagccctac cataacagtt
     1261 tcttcatccg tatcaaaaat taaatctatt cctttagact tagcataact actaatagat
     1321 gtaactattc tttctgttat gtcaataata tcaaaattaa ctgctttaat cttatatatg
     1381 tctgacttaa cttgagaaat atttattata ttatctatta atctcaataa tctaaacata
     1441 ttttgtttag ttatttttat atgttctttt agtatatcct gaaaatcaat tatttttcct
     1501 tttttataaa ggttttcaat aagttgaact gaactatata tattatttaa aggtgtctta
     1561 agttcatgag atatattagc ataaaactcc atcttcattt ggtcatactg tctttgtttt
     1621 attaatgctt ttttaagtct ttcagactct ctttgcacac ttaaatcttt aataattctc
     1681 actctacttc ttttctgtcc aaagtataat gttcttgata tcatttttac ttctttaatg
     1741 tgattatttt catttactat atggatactc ttctctcttt ctcctatatt taaattgttt
     1801 atgtattttt ttactaatat atttctatat ttttgtgttg taactcctaa tacatcacta
     1861 gaaattccga acaactctct aaaggctcta tttgtatata gtactcttga tgtacttaaa
     1921 tcctcaagaa aaatagcctc tggtataaca tcaagtaatt tttcatatat tattttttgt
     1981 tcatttactg ttctaagaat atttatctct ttagttatat ctctaaatgt aagcaattta
     2041 aagagtttat tatctgacca atagtctcca actctaactt cttcaacatg ttcttcacct
     2101 tctgagtcag acatcacaaa attaactata cagtttgctg tgtcattgta acttactatt
     2161 tcagctttct tttcttgtgt tagatttaat cttttaaatt tcctattgtc aaagaaatct
     2221 atgttaaaag tttcaaaata ttgttctaat gtcatatcga ctaaatcact tgaaacacat
     2281 ttaaaactct tttcatattt tttatttata cttattacct tattcttctt tatgccacct
     2341 tcagcacttc ttacaagtat aggagatgga atatttagta taatgttttc atagtcttta
     2401 tttatatttc ttatttcttc atctattttg atttcatttg taatatcatt taaacttatt
     2461 aagtaattgc tttcatcttt attattactt aaacttaaca aataaatttt atcatttaac
     2521 tctaccagtt tatcaattat acttatatcg ttatcaaatt tcttcaattt ttcagcaact
     2581 ttttcatctt cctttataaa agttaatatt ttataataat tattcatatc ctttgaaaat
     2641 tcatctttaa atttattatt tgtgtaactt atattaaaat catagtctgt tactatccat
     2701 acatttggta ttgtatccag agcttgtcta agtaattctt ttctctggtc tatgtagttt
     2761 tttaatgttc tttgtataat catatccttt tctattgcta aatttatctt tcctaattgc
     2821 tctgactttt tacttaattt tttattgatt gaatcaagtt cataatatgg atacactact
     2881 ctattattaa gactgtatac atatacataa taagtatggt tacataatag taaatatatt
     2941 actcctatta atctatctga atctactata ttaatcttag aaattgctac aaaacatata
     3001 atagttgtaa aaaaaacaaa atatgttaaa tcttttatta acttatagtg ctcaatgtta
     3061 ctatcaagtt tattcaacaa atatagtaaa ttaaacatag atatacaaat attaattaat
     3121 ataataatca tattccagtt aatatcacta aaaatatttc ttataaagta atctaatatc
     3181 acaaaagcac tataaaaaat tagtattggg cttattctta gtttttttac tttagaaatg
     3241 gttaagtata taaaagtaga catattaatt atgcttaagt taagcaatac aatttggaat
     3301 ttttgaattt ttaaatttac tactcctaaa atacttacaa aaactaatac aagacaccct
     3361 actttcaata tatttataaa ttcatctgtt ttaaagctac ttatataaac tatagaatat
     3421 ccaaatatcc aaagtaaaat tattctatac atatttatag aaactgtata caaatttata
     3481 tttaaaagtg caataaccaa aaataaccca ttagctagta tatagacctt attcttactt
     3541 aaagttcctt tagattccat attccccacc acttcttcat ttttatatag ttttacctat
     3601 aaatatgtta ccacaattct tccaaaaaat ttgtcacttt ctagttataa aaaaaaatga
     3661 gtaaatattt ttaattttac tcattttgta gattttgtat attcagttta aaaagctgga
     3721 actatacttc ctttatattc atcttgtata aattttttaa cttctttact attcatagcc
     3781 tctgataaag ccttaatctt atcactatct ttattatttt ctctacaagc tattatattt
     3841 acatatggag aatcacttga ttctattact attgcatcct tagtaggatt taagttagct
     3901 gttaaagcat aattagaatt tattactgct ccgtcaacat cttttaatac tgttggtaat
     3961 tgctctgcat tcatttcttt tattactata tttttaggat ttttagttat atctttttta
     4021 gttataagct ctccatcttt tacttctatc aatttatttt ttgctaataa tttaagtgct
     4081 ctagctccat tagttgcatc atttggcaca gctataacag ctccatcttt taattcatct
     4141 aatgacttaa ctttttctga atagaatccc attggctcta tatgaacttt agatgtataa
     4201 gtaagtttat atcctttctc cttcacagtt tcctctaaaa atggaatatg ttgaaagaag
     4261 ttagcatcca aagacccttc atctaaagct gtatttggaa gtacataatc atcaaatatt
     4321 tttacttcta aatcatatcc tttttctttt aacaaaggtt ttgcaacttc caatatcttt
     4381 gcatgtggat ttgatgaagc tcctactagt atttttttat cctctttgtt tgaacaacct
     4441 actgctgata ttgctattgc tgacaccaat gctactgata ataatttctt taatttcatt
     4501 tttactcccc ctctttaaaa tgtttataat taatttattt tagtttctta taagccagat
     4561 ttcctactcc ctggattatc tgaactaata ttataagtgc tattacagta tataccatta
     4621 ctgctgtatc aaatctttga tatccataaa taagtgctat attacctaat cctccagctc
     4681 ctactgcacc tgccatagct gtataaccaa gtatagatat tattgataat gtaattccag
     4741 atactattga aggcatagct tcctttatca taactttaaa tactatttgt ctttttgtag
     4801 ctccaaaaga ttttgctgct tctatcaatc ctttatcaac ttcatttaat gagctttcta
     4861 taattctagc gataaacggt gctgccccaa tagttattgg cactattgca gcagtttccc
     4921 ctatacttgt tccaacgatt aatctagtta ttggaatcat tgcaactata agtatcataa
     4981 atgggaaact tctaaatata tttactataa atcctaatgc actatttatt gtgctatttg
     5041 gttttaatcc atctggtttt gtaactacta atatgattgc taatataaat cctaatattg
     5101 ttgccactat agttggtact ataaccatat acaaagtttg taacaatgca tttggaaata
     5161 gtgttgttaa aaagtcaatt aaactattca tttatcattt cctcccatct tatgccctta
     5221 ctagttagat attgttttac gtgctctcca cttttatctg aaatgtttat aataagtgaa
     5281 cctaaaatat catctttaaa ttgttctaat ttcccaaata ttatagacac atctatattt
     5341 agttctcttg ccatagtcgt aataatagct tcattagaaa tatctttagg gaatagtatc
     5401 tttatatttg ttccttttgg caatattata ctttcttcac ctattaattt tctaagtcct
     5461 ttagtatttc ttaagaatat ttcttctgta tctcctacct ctaatacttc tccattttcc
     5521 attattgcta ctcttccaca aatctgcttt ataacttcca tttgatgagt tacaactatt
     5581 attgttattc ccaatttttt atttatatct tcaagcaatg aaagaattga ctttgtagta
     5641 tttgggtcta atgctgaagt tgcttcatca caaagaagta cttgtgggtt tagtgctaaa
     5701 gctctagcaa ttgcaaccct ttgtttttgt ccgccactta gatttctggg tttatcattt
     5761 ttcttttcag atattccaac tacttctaat aactctaaaa ctcttttttt aatttctgcc
     5821 tttgagtatc caaaacattc taatggcaat gctacattat caaatacagt ttttctttca
     5881 agcaatgaaa agtgttgaaa aatcattcct aactcttttc ttaaatctct catttgcttt
     5941 tcattcaatg actttacttc tttatcagaa acaagtacac tgccttcttg gtattcttct
     6001 aatccgttta tacatctcag tagtgttgat ttccctgcac cactatgacc tattattcca
     6061 aaaatttctc cagattctat ttcaatactt acatctttaa gcacttgaat ctttccataa
     6121 tacttattaa cattttttat gctaatcata aaatttcctc ccatatattc tgctcagttt
     6181 ttatgtttat cctctcttat gtattacaac aaaataaaag cccgagtata aactcaggct
     6241 tagaaagcta tttatactca tctatcaact atattagttg caggatttag caccaatgta
     6301 tataaaatat actggttgcc gggtttcaaa gggccagtcc ctccaccact cttgataaga
     6361 cgttatttaa ttcaattcta ttatcatttg ttgctatgtt atatatgata tattcattaa
     6421 tagtatttgt caataaattt atttaaattt tttatttttt attatcgtta attaactatt
     6481 tttaattagt tttctagctc ctaacacttt atattttaat gtctatataa ctatagaaca
     6541 tcattataag aaataaatta aataacatca atctatctaa atcatctgta aagtaaatta
     6601 agaaaattaa cattttatcc aaactctatc ctactattaa ataaaataat ttaaatacaa
     6661 tgatgattat atagctctaa tactttagac t
//
LOCUS       DACTGW010000195         6598 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.195, whole genome shotgun sequence.
ACCESSION   DACTGW010000195 DACTGW010000000
VERSION     DACTGW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.195"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            98..>6598
                     /gene="tcdA"
                     /locus_tag="I9573_002994"
                     /gene_synonym="toxA"
     CDS             98..>6598
                     /gene="tcdA"
                     /locus_tag="I9573_002994"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HAT4797592.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     LLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTIPRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKDSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKEYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPYISSHIPSLSV
                     YSAIGIKTENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENNGTKLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNTKIKLDKDTRNFIMPTITTDEIRNKLSYSFDGA
                     GGTYSLLLSSYPISMNINLSKDDLWIFNIDNEVREISIENGTIKKGNLIEDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNGSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLNNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIESNLVTGWQTINGKKYYFDINTGAASTSYKIINGKHFYFNNNGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSGSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTSIASTGYTIINGKYFYFNTDGIMQIGVFKVP"
BASE COUNT         2672 a          768 c          975 g         2183 t
ORIGIN      
        1 agtttaaaaa caagaaaatc aatttaaatt tcagaaggaa taaatgtggt tatagaagtg
       61 gatttattat caaaaataat agtactagga ggtttttatg tctttaatat ctaaagaaga
      121 gttaataaaa ctcgcatata gcattagacc aagagaaaat gagtataaaa ctatactaac
      181 taatttagac gaatataata agttaactac aaacaataat gaaaataaat atttacaatt
      241 aaaaaaacta aatgaatcaa ttgatgtttt tatgaataaa tataaaaatt caagcagaaa
      301 tagagcactc tctaatctaa aaaaagatat attaaaagaa gtaattctta ttaaaaattc
      361 caatacaagt cctgtagaaa aaaatttaca ttttgtatgg ataggtggag aagtcagtga
      421 tattgctctt gaatacataa aacaatgggc tgatattaat gcagaatata atattaaact
      481 gtggtatgat agtgaagcat tcttagtcaa tacactaaaa aaggctatag ttgaatcttc
      541 taccactgaa gcattacagc tactagagga agagattcaa aatcctcaat ttgataatat
      601 gaaattttac aaaaaaagga tggaatttat atatgataga caaaaaaggt ttataaatta
      661 ttataaatct caaatcaata aacctacagt acctacaata gatgatatta taaagtctca
      721 tctagtatct gaatataata gagatgaaac tttattagaa tcatatagaa caaattcttt
      781 gagaaaaata aatagtaatc atgggataga tatcagggct aatagtttgt ttacagaaca
      841 agagttatta aatatttata gtcaggagtt gttaaatcgt gggaatttag ctgcagcatc
      901 tgacatagta agattattag ccctaaaaaa ttttggcgga gtatatttag atgttgatat
      961 gcttccaggt attcactctg atttatttaa aacaatacct agacctagct ctattggact
     1021 agaccgttgg gaaatgataa aattagaggc tattatgaag tataaaaaat atataaataa
     1081 ttatacatca gaaaactttg ataaacttga tcaacaatta aaagataatt ttaaactcat
     1141 tatagaaagt aaaagtgaaa aatctgagat attttctaaa ttagaaaatt taaatgtatc
     1201 tgatcttgaa attaaaatag ctttcgcttt aggcagtgtt ataaatcaag ccttgatatc
     1261 aaaacaaggt tcatatctta ctaacctagt aatagaacaa gtaaaaaata gatatcaatt
     1321 tttaaaccaa caccttaacc cagccataga gtctgacaat aacttcacag atactactaa
     1381 gatttttcat gattcactat ttaattcagc taccgcagaa aactctatgt ttttaacaaa
     1441 aatagcacca tacttacaag taggttttat gccagaagct cgctccacaa taagtttaag
     1501 tggtccagga gcttatgcat cagcttacta tgatttcata aatttacaag aaaatactat
     1561 agaaaaaact ttaaaagcat cagatttaat agaatttaaa ttcccagaaa ataatctatc
     1621 tcaattgaca gaacaagaaa taaatagtct atggagcttt gatcaagcaa gtgcaaaata
     1681 tcaatttgag aaatatgtaa gagattatac tggtggatct ctttctgaag acaatggggt
     1741 agactttaat aaaaatactg ccctcgacaa aaactattta ttaaataata aaattccatc
     1801 aaacaatgta gaagaagctg gaagtaaaaa ttatgttcat tatatcatac agttacaagg
     1861 agatgatata agttatgaag caacatgcaa tttattttct aaaaatccta aaaatagtat
     1921 tattatacaa cgaaatatga atgaaagtgc aaaaagttac tttttaagtg atgatggaga
     1981 atctatttta gaattaaata aatataggat acctgaaaga ttaaaaaata aggaaaaagt
     2041 aaaagtaacc tttattggac atggtaaaga tgaattcaac acaagcgaat ttgctagatt
     2101 aagtgtagat tcactttcca atgagataag ttcattttta gataccataa aattagatat
     2161 atcacctaaa aatgtagaag taaacttgct tggatgtaat atgtttagtt atgattttaa
     2221 tgttgaagaa acttatcctg gtaagttact attaagtatt atggacaaaa ttacttccac
     2281 tttacctgat gtaaataaag attctattac tataggagca aatcaatatg aagtaagaat
     2341 taatagtgag ggaagaaaag aacttctagc tcactcaggt aaatggataa ataaagagga
     2401 agctattatg agcgatttat ctagtaaaga atacattttt tttgattcca tagataataa
     2461 gctaaaagca aagtccaaga atattccagg tttagcgtca atatcagaag atataaaaac
     2521 attattactt gatgcaagtg ttagtcctga tacaaaattt attttaaata atcttaagct
     2581 taatattgaa tcttctattg gtgattacat ttattatgaa aaattagaac ctgttaaaaa
     2641 tataatccac aattctatag atgatttaat agatgagttc aatctacttg aaaatgtatc
     2701 tgatgaatta tatgaattaa aaaaattaaa taatctagat gagaagtatt taatatcttt
     2761 tgaagatatc tcaaaaaata attcaactta ttctgtaaga tttattaaca aaagtaatgg
     2821 tgaatcagtt tatgtagaga cagaaaaaga aattttttca aaatatagcg aacatattac
     2881 aaaagaaata agtactataa agaatagtat aattacagat gttaatggta atttattgga
     2941 taatatacag ttagatcata cttctcaagt taatacatta aacgcagcat tctttattca
     3001 atcattaata gattatagta gcaataaaga tgtactgaat gatttaagta cctcagttaa
     3061 ggttcaactt tatgctcaac tatttagtac aggtttaaat actatatatg actctatcca
     3121 attagtaaat ttaatatcaa atgcagtaaa tgatactata aatgtactac ctacaataac
     3181 agaggggata cctattgtat ctactatatt agacggaata aacttaggtg cagcaattaa
     3241 ggaattacta gacgaacatg acccattact aaaaaaagaa ctagaagcta aggtgggtgt
     3301 tttagcaata aatatgtcat tatctatagc tgcaacggta gcttcaattg ttggaatagg
     3361 tgctgaagtt actattttct tattacctat agctggtata tctgcgggaa taccttcatt
     3421 agttaataat gaattaatat tgcatgataa ggcaacttca gtggtaaact attttaatca
     3481 tttgtctgaa tctaaagaat atggccctct taaaacagaa gatgataaaa ttttagttcc
     3541 tattgatgat ttagtaatat cagaaataga ttttaataat aattcgataa aactaggaac
     3601 atgtaatata ttagcaatgg aggggggatc aggacacaca gtgactggta atatagatca
     3661 ctttttctca tctccatata taagctctca tattccttca ttatcagttt attctgcaat
     3721 aggtataaaa acagaaaatc tagatttttc aaaaaaaata atgatgttac caaatgctcc
     3781 ttcaagagtg ttttggtggg aaactggagc agttccaggt ttaagatcat tggaaaataa
     3841 tgggactaaa ttgcttgatt caataagaga tttataccca ggcaaatttt actggagatt
     3901 ctatgccttt ttcgattatg caataactac attaaaacca gtgtatgaag acactaatac
     3961 taaaattaaa ctagataaag atactagaaa ctttataatg ccaactataa ctactgacga
     4021 aattagaaac aaattatctt attcatttga tggagcagga ggaacttact ctttattatt
     4081 atcttcatat ccaatatcaa tgaatataaa tttatctaaa gatgatttat ggatatttaa
     4141 tattgataat gaagtaagag aaatatctat agaaaatggt actattaaaa aaggaaattt
     4201 aatagaagat gttttaagta aaattgatat aaataaaaat aaacttatta taggcaatca
     4261 aacaatagat ttttcaggtg atatagataa caaagataga tatatattct tgacttgtga
     4321 gttagatgat aaaattagtt taataataga aataaatctt gttgcaaaat cttatagttt
     4381 gttattgtct ggggataaaa attatttgat atccaattta tctaatacta ttgagaaaat
     4441 caatacttta ggcctagata gtaaaaatat agcttacaat tacactgatg aatctaataa
     4501 taaatatttt ggagctatat ctaaaacaag tcaaaaaagc ataatacatt ataaaaaaga
     4561 cagtaaaaat atattagaat tttataatgg cagtacatta gaatttaaca gtaaagactt
     4621 tattgctgaa gatataaatg tatttatgaa agatgatatt aatactataa caggaaaata
     4681 ctatgttgat aataatactg ataaaagtat agatttctct atttctttag ttagtaaaaa
     4741 tcaagtaaaa gtaaatggat tatatttaaa tgaatccgta tactcatctt accttgattt
     4801 tgtgaaaaat tcagatggac accataatac ttctaatttt atgaatttat ttttgaacaa
     4861 tataagtttc tggaaattgt ttgggtttga aaatataaat tttgtaatcg ataaatactt
     4921 tacccttgtt ggtaaaacta atcttggata tgtagaattt atttgtgaca ataataaaaa
     4981 tatagatata tattttggtg aatggaaaac atcgtcatct aaaagcacta tatttagcgg
     5041 aaatggtaga aatgttgtag tagagcctat atataatcct gatacgggtg aagatatatc
     5101 tacttcacta gatttttcct atgaacctct ctatggaata gatagatata tcaataaagt
     5161 attgatagca cctgatttat atacaagttt aataaatatt aataccaatt attattcaaa
     5221 tgagtactac cctgagatta tagttcttaa cccaaataca ttccacaaaa aagtaaatat
     5281 aaatttagat agttcttctt ttgagtataa atggtctaca gaaggaagtg actttatttt
     5341 agttagatac ttagaagaaa gtaataaaaa aatattacaa aaaataagaa tcaaaggtat
     5401 cttatctaat actcaatcat ttaataaaat gagtatagat tttaaagata ttaaaaaact
     5461 atcattagga tatataatga gtaattttaa atcatttaat tctgaaaatg aattagatag
     5521 agatcattta ggatttaaaa taatagataa taaaacttat tactatgatg aagatagtaa
     5581 attagttaaa ggattaatca atataaataa ttcattattc tattttgatc ctatagaatc
     5641 taacttagta actggatggc aaactatcaa tggtaaaaaa tattattttg atataaatac
     5701 tggagcagct tcaactagtt ataaaattat taatggtaaa cacttttatt ttaataataa
     5761 tggtgtgatg cagttaggag tatttaaagg acctgatgga tttgagtatt ttgcacctgc
     5821 caatactcag aataataaca tagaaggtca ggctatagtt tatcaaagta aattcttaac
     5881 tttgaatggc aaaaaatatt attttgataa tgactcaaaa gcagtcactg gatggaggat
     5941 tattaacaat gagaaatatt actttaatcc taataatgct attgctgcag tcggattgca
     6001 agtaattgac aataataagt attatttcaa tcctgacact gctatcatct caaaaggttg
     6061 gcagactgtt aatggtagta gatactactt tgatactgat accgctattg cctttaatgg
     6121 ttataaaact attgatggta aacactttta ttttgatagt gattgtgtag tgaaaatagg
     6181 tgtgtttagt ggctctaatg gatttgaata tttcgcacct gctaatactt ataataataa
     6241 catagaaggt caggctatag tttatcaaag taaattctta actttgaatg gtaaaaaata
     6301 ttactttgat aataactcaa aagcagttac cggatggcaa actattgata gtaaaaaata
     6361 ttactttaat actaacactg ctgaagcagc tactggatgg caaactattg atggtaaaaa
     6421 gtattacttt aatactaaca ctgctgaagc agctactgga tggcaaacta ttgatggtaa
     6481 aaaatattac tttaatacta acacttctat agcttcaact ggttatacaa ttattaatgg
     6541 taaatatttt tattttaata ctgatggtat tatgcagata ggagtgttta aagtacct
//
LOCUS       DACTGW010000196         6572 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.196, whole genome shotgun sequence.
ACCESSION   DACTGW010000196 DACTGW010000000
VERSION     DACTGW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.196"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..656)
                     /locus_tag="I9573_002995"
     CDS             complement(<1..656)
                     /locus_tag="I9573_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797593.1"
                     /translation="MKKDTSHIIKRAEHFQVILIGEGLMVGAVAGLIVLLYRILLGQA
                     GTWLSQILKFVEGSTIKIIAWFAVLAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKI
                     KQVWWRVLPTKFIGGFLSLMGGLALGREGPSIQLGAMAGKGISRALDRGKTEEKFLLT
                     CGASAGLSAAFHAPLAGVMFSLEEVHKNFSVSVLISVMTASLTADYISANFLGMESVF
                     "
     gene            complement(843..1352)
                     /locus_tag="I9573_002996"
     CDS             complement(843..1352)
                     /locus_tag="I9573_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HAT4797594.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLE"
     gene            complement(1427..1840)
                     /locus_tag="I9573_002997"
     CDS             complement(1427..1840)
                     /locus_tag="I9573_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HAT4797595.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNSDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2277..2936
                     /locus_tag="I9573_002998"
     CDS             2277..2936
                     /locus_tag="I9573_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAT4797596.1"
                     /translation="MDEKILELLQEIQGNISNMQGSISGIEGTVNGMQGNINYMQGNI
                     NYMQGTISDMQGTISDMQGSINGIEGTISDMQGSINGIEGTISDMQSNINDIEGTISD
                     MQGSISSMDGTINSMQDSITDMQGEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQ
                     SDVTEIKSDVKINRNTLDTVIKHTSSLVEDTSDIKNDLNNIEIVTSKNWNDIAKLKSV
                     K"
     gene            complement(3543..3722)
                     /locus_tag="I9573_002999"
     CDS             complement(3543..3722)
                     /locus_tag="I9573_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT4797597.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(3947..4480)
                     /locus_tag="I9573_003000"
     CDS             complement(3947..4480)
                     /locus_tag="I9573_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAT4797598.1"
                     /translation="MQYISYYHSPIGNILLASDNVGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGEEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     HKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(5298..5870)
                     /locus_tag="I9573_003001"
     CDS             complement(5298..5870)
                     /locus_tag="I9573_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAT4797599.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGSCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(5932..6195)
                     /locus_tag="I9573_003002"
     CDS             complement(5932..6195)
                     /locus_tag="I9573_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HAT4797600.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDLYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6195..6416)
                     /locus_tag="I9573_003003"
     CDS             complement(6195..6416)
                     /locus_tag="I9573_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HAT4797601.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYSNPKNRVRAKKGGNMY"
BASE COUNT         2302 a         1130 c          812 g         2328 t
ORIGIN      
        1 tggaatacag actccattcc taggaaattg gctgaaatat agtctgctgt caaagatgct
       61 gtcatcacag aaattaaaac agatactgaa aagtttttat ggacttcttc aagtgaaaac
      121 ataacacctg ccagtggtgc atgaaaggct gctgataatc cagcacttgc cccacaggtt
      181 aacaggaatt tttcctctgt cttccctcgg tcaagagctc tagaaatacc tttacctgcc
      241 atagcaccaa gctgaatgga aggaccttcc cttcccaaag caagaccacc cataagtgat
      301 aagaatcctc caataaattt tgtgggcaac actcgccacc atacttgttt tattttccct
      361 gtcatttccc cctcaagctg agggatacca cttccagaaa tcattggctc ccagtccacc
      421 aactttgata caatccacgc aagtactgca agtactgcaa accaagctat tatttttatt
      481 gtagaacctt ctacaaattt taaaatttgg ctaagccaag taccagcctg ccctaaaagt
      541 atcctgtata aaagcacaat caaaccagcc acagccccaa ccataagtcc ttcaccaatc
      601 aaaattacct gaaagtgctc tgcccgtttg ataatatgag atgtatcctt tttcatactg
      661 ttcctccttc atccaataat tctacacaat ctgaacctcc aatgactaaa gtcatgagtg
      721 tgtacagata ttttttaatc aacaaaaata gatttttatt tactaaatat aagaaaataa
      781 ttgttcaatt tctcaataaa tgtaaattta ttcttttagg tatttattct ccacagtcgt
      841 gcttattcca gcaaaatatt tattagttct tgctttctct gtgtatagat tttacgattg
      901 tcctcaaact gtaatgctaa tttttgttta tgactatcat agagttttgc tttgtctgta
      961 aaagtattta gataatctcg aaaattgata taattattcc atgcgacact attccattca
     1021 acaacatgaa tatggtgagt acgagtattt ttctcaaaat ctcccatagc aaaaagtaat
     1081 tgttttggta catcctgacc tcgaaaaacg attccacatt gctctaacct ttcgaggtat
     1141 aatttagctt cctctaaaga agcaacacct acaacaatat caataatagg ctttgcgcta
     1201 atactgggaa tagaagtgct tccaatgtgt tgaatatcaa tggcagtctt atcccatata
     1261 cttttcaata atataatgac atcttttgca cttttatccc atatcatctg atgttccaat
     1321 aatttaacca taccacattt taaacctatc actatatacc acctcaatat aatccacctt
     1381 tatatatata ttacaatttt ttctatcttg ataaacagtg atctagctat ttcacttttt
     1441 cttgacactc gctacattcc ctatcatgaa tagatataat tcctccacat ttagaacaag
     1501 tatatctctc cttctgcttt tgcataaact tctctaaacc gtactgctga acaaatttac
     1561 tattttccat aaggcttgct tgatatctct tgttataact tttttcaaga ttttttatga
     1621 gtttacaagg aaaattgaaa cattcaaaac aataaaacaa ccctttttga cagacacaat
     1681 cctttatttt acacttacgg cagtgttctg gctttcctgt atcgctgttc aaacaaccag
     1741 cacatggctt tttgtgataa caatgcttat agcaaaccat acaattcatt ccacaaggag
     1801 caaacataat cgtatcaata ctctcttttg gcattttcat aacattttac tcctttcaaa
     1861 aatccaattt gtcgaattgt tatatattca tattgtaaca taaaaaatag ctaaaaaatt
     1921 catgcacttt cattgccaat aaattttcaa ggaaatatga aagtttaaaa ctaagaataa
     1981 aaagaaataa aaaggaagct actaatcaaa atattcaaac acaagtaatc aattatgata
     2041 ttaccagcag ttttaaatac aaatataacc acatatgcct caaaaacact tattatagta
     2101 tcttagacac ttagacaata cacaagattc attaataaaa aagttctcaa tatagatata
     2161 tttaaccatg ttttgaatag cagataatta tatgattaaa aattaaatat gacatagcgt
     2221 ttgtggaata taacacctaa attatgttat aataataaaa caggagtgtg atagtgatgg
     2281 atgaaaaaat attagaatta ttacaggaaa tacaaggtaa tattagtaat atgcaaggta
     2341 gtatcagtgg tatcgaaggt actgttaatg gcatgcaggg taatattaac tatatgcagg
     2401 gtaatattaa ctatatgcaa ggtactatta gcgatatgca aggtactatt agcgatatgc
     2461 aaggcagtat caatggtatc gaaggcacta ttagcgatat gcaaggcagt atcaatggta
     2521 tcgaaggcac tattagcgat atgcaaagca atatcaatga tatcgaaggt actattagcg
     2581 atatgcaagg cagtatcagt agtatggatg gcactattaa tagtatgcaa gacagtatta
     2641 ctgatatgca aggtgaaatt aaagaagtca atagtagatt agacaatatg gaacctgata
     2701 ttaaagactt aaaaactgga caagttgaaa ctagtagtag acttgataca attcagagtg
     2761 atgttacaga aattaagtca gatgtaaaaa ttaacagaaa tacattagat acagtgatta
     2821 aacatacctc ttcattagta gaagatacat cagatattaa aaatgattta aacaatatag
     2881 agatagtcac atctaagaat tggaatgata ttgctaaatt aaaatcagtt aaataatata
     2941 ccatagtaaa aaatacctga aaatttcaca tacttcttta aagttatacc aaattttata
     3001 cagaaatact gattatccga caacacactt tacttataaa taatttatta aaaaatactc
     3061 attttatcct aaaattaaca cgactaaatg agtcttgata taataataaa aaataaggga
     3121 gattgaacac taaaaagttg tgtatctccc ttatattcta taatatagtt atataatttt
     3181 tcagcctaaa ataaaagtcc atctttgatt aaaacatatc cccttatcac ttataaaagg
     3241 ataaaatctt gttttcattt ttccatttat atcttcttgc ttaagagtaa taattttttc
     3301 ttttgatact ttaagtgttt ggttaaattt aaccaatcga ctaagttttg ttcaacatct
     3361 atttttcata ttttccaata cttaatctat tttttataaa taattctttt atttctaact
     3421 cacttacatt ttttacataa aacgaagcat ataaatttca accttatctt gtatggttct
     3481 taatacttaa tgagtagctt aagtatcaat cattataaat tttcccctta tatttaacac
     3541 tcttaccatt tccaaaatat acaaaatata ttttgaacat ttgtacaatg atgaaataca
     3601 atacatgaaa atacaatatc ataactgtta tctaaaaata gaagttcttc aacataacct
     3661 ttcacaaaga acatattatc taagtatttt ttgtctgctc cttgttttcc tgtttgtagc
     3721 atataaatta ctgcatctaa acatataact actcacacga aaaaagaaaa taaacttcca
     3781 aaatacaagc tctaaaacta cctctaaagt tatataatgt ttactaggaa taaggttgta
     3841 taacatatag tttaaagaat agttttttaa aattgcaagt tattgctttc aaaattagat
     3901 gtttatatat caaataaatg attaagaatt tctaaattta atggctttat aatgcactcc
     3961 ctttctttgg gataaaataa tgttgcacag atactttttc taaagataac aattttattt
     4021 ttctatcaat tccaccagca taccctgtca aactcccatt tgttcctaca acacgatgac
     4081 atggaacaat aatagagatt ccattatgcc ctactgcacc accgattgct tgtgctgaca
     4141 ttttagatat tcctttttgt tgagcaactt tatgtgcaat ttctccataa gtgattgttt
     4201 taccatatgg tatttgttgt aaaattttcc acactgtagt ctggaaagga gtacctacca
     4261 tatggagtga tggtgtaaaa ttgggctctt ctcccgaaaa ataaatatct agccatttct
     4321 ttgtctgctc gaaaattggc aattcttttt cttgatgttc ttggtcaaga ttataagcat
     4381 aatacttttg attctcaaac caaagaccag ttaacccaac attatctgat gctaaaagaa
     4441 tatttccaat tggactatgg taatagctaa tatattgcat acaattcctc cttatattct
     4501 gaaaacttca ctcaaatcat tagacacttc ttttctatct gccattatgt aaaaacatac
     4561 taatataatt tccttctgac tacactttcg ttataatatc tatcctcata tcatattagt
     4621 tttatattta cctgaatctg ttcatccttt ttttcctttc cctttgatgg atataaaatt
     4681 catacattta agcttcctac tgtattaact tttatagaag tgtatattat aattttatca
     4741 ctaaatagac aaaaaaatat cgatataaca tcaagaaata ttttactgag tttcgcttat
     4801 taaaagcaac tgaatttcta aagggtacag atgagctaat aagtgatatt gatataaaag
     4861 ttgtttttca ttaagtaatc tattttagta aatgttttaa agaaaaaacg ggatattctc
     4921 caaaagtgta cagaaatata aaaaaagaga tatgattttt tatttctttt gattatccaa
     4981 agagtaaata aaaaatattg taaaaaactt actaacaacg gcaatgcttt gaactatggt
     5041 aatactttga acttttgatt ggactttttt tgctaaaata atatatttaa atagcaaagt
     5101 aatctgttta atcaataaaa aataagagat aaaagaaatc aactaaaacc tttacctctt
     5161 attttgatag tttatcagag ctaatattta cactcttttt atttttgata cttcattaaa
     5221 aatgatgctt attctctttt catttttaat acttcattaa aattgatgct tactctctat
     5281 tcacttttaa tacttaatta gaactgatgc ttacactctg taaactcata aacatccatt
     5341 aatgcttcac caaatctttg atagtgaact acttccctct cacccaaaaa tctcaatata
     5401 tctttcaaat cagggtcatc agttaaattt attaattgat agtacgtagc caatgctttt
     5461 tgttcagcag ccatatcctc atgtaaatca gttaccgaat cgccaaagac attaatataa
     5521 cttgtagtaa agtttactcc attagaatct actggttgaa gaccatgtcc aaaattagca
     5581 taattactac caagtccagc agccttaagc tcttccggag tacaactgcc tattaattga
     5641 tataacactg aagaaattat ctcaacatgg gccatttcct ctgtacctat atcagttaaa
     5701 agtgcgcgag attttccagt tggcatagta tatctttgtt gaagatatct agttgcagct
     5761 gccaactcac cattaggtcc tcccaactga gtcatgatat attttgccat tctagggtct
     5821 gcttgtctta tgttaactgg atgttccagt gttttttgat aaatccacat attatcttac
     5881 tcctccttaa tatatattta ataataattc taattttaaa acttaataat tttatttata
     5941 aaatgatttg tcccatggcc aaggttcaga tatccatgac caaggatatt cacttgattc
     6001 atatccaaaa ttagttaatg ctccatattt agattcataa tcacacacag cttgtgtaaa
     6061 tttatcgcag tatctattgt ataaatcaag agctttctta tcccttggat tgttatctaa
     6121 atataaattt aattctatac aggcaaattg atattctgaa attctctcta aaagttctct
     6181 tctatttaat gacattagta catgttaccc ccttttttag ctctaactct atttttagga
     6241 tttgaatata gccaatcatt atagttattt gactctatta aatctaattc aggaaataaa
     6301 gtacctcttt ttaatgctgt ttttggattg tatgtgtcat cataaatttg acgattgatg
     6361 tatggtctag caaattcaca acaatagtct tgtttatcct cttttaaata tcccattaat
     6421 tatcctcctt aatttttaga attttaaaat agcattattt ttaaaaattg tatacttgtt
     6481 ctatattata gtcagtagtt ttaataggtc tttaataaga ttaaataatt aaataagtat
     6541 ctttttttgt tcttatttta cctataaaaa tt
//
LOCUS       DACTGW010000197         6546 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.197, whole genome shotgun sequence.
ACCESSION   DACTGW010000197 DACTGW010000000
VERSION     DACTGW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.197"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            211..1404
                     /locus_tag="I9573_003004"
     CDS             211..1404
                     /locus_tag="I9573_003004"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAT4797602.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            1610..2728
                     /locus_tag="I9573_003005"
     CDS             1610..2728
                     /locus_tag="I9573_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HAT4797603.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            2859..3104
                     /locus_tag="I9573_003006"
     CDS             2859..3104
                     /locus_tag="I9573_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HAT4797604.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            3091..3837
                     /locus_tag="I9573_003007"
     CDS             3091..3837
                     /locus_tag="I9573_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT4797605.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            4122..4997
                     /gene="hslO"
                     /locus_tag="I9573_003008"
     CDS             4122..4997
                     /gene="hslO"
                     /locus_tag="I9573_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAT4797606.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPDDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            5388..6188
                     /locus_tag="I9573_003009"
     CDS             5388..6188
                     /locus_tag="I9573_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HAT4797607.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
BASE COUNT         2616 a          621 c         1270 g         2039 t
ORIGIN      
        1 atatttatat tatattaata ttagaaaaat tttaaataaa tgttttattc tcaaaatatg
       61 catattaatt taataaaata taccaaaatg gaaataaaaa ataaatttta tgagaattta
      121 ataactaata attaatatat ttaggttata ataaatatgt gcgtaaaatc taacaataag
      181 caataatacc gtaaatttag gaggaataat atggatactt taaaggaact ttttaaaata
      241 ggaagtggac cttcaagttc tcatactatg ggaccacaaa gagcagcaga gagatttaag
      301 aatgaaaacc cagatgcaga aagttttaga gcaatattat atggtagttt ggcagctaca
      361 gggaaaggtc acttaactga ctatataatt gaaaaaacta tagcacctaa aaaagtcgaa
      421 attgtttggg aagaagatat tataaaagat tttcacccaa atgggatgaa gtttgaagct
      481 ttagataaag ataaaaatgt aacagctgaa tggactgtat attctgtagg tggagggact
      541 atagcagaag aaggtcaaag aaacagtaaa agcaatagta tatatcactt agatactatg
      601 gatgagatag taaaatggtg caaagaaaat aataagactt tggtagattt tgtgttggaa
      661 tgtgaaccaa aagacataaa agattatata aaaactataa aagatgctat gagaaaatct
      721 attgatgatg gactaagtac agatgaaata ataccaggca agttattatt aaaaagaaga
      781 gcaagtacat tttataatgc atataaaaaa gataagagct tctcaacact tgtatatgca
      841 tatgcactag cagcttcaga gcaaaacgct tctggtaata taatagttac agcaccaact
      901 tgtggttctg ctggagtaat accaggaata ttctttgcaa tgcaggattt ctataattat
      961 gatgacgaaa aaataattga agcactactt gtagctggaa taataggtaa tataataaaa
     1021 actaatgctt caatatctgg agcagaggta gggtgtcaag gtgaagttgg agcagcttgt
     1081 tctatggcag cagcagcagt agcatactta aaaggtggaa ctatagacca tattgaatat
     1141 gcggcagaaa tagcacttga acatcattta ggaatgactt gtgaccctgt atatggatat
     1201 gttcaaattc catgtataga aagaaatgca atggcagctc aaagagctta tgatgcagct
     1261 aattatgcat tattaactga tggctcacat tctgtctcct tagaccaagt tgttgaaaca
     1321 atgaaagaaa ctggtataga tatgatggat aagtacaaag aaacagctaa gggtggactt
     1381 gctaaacatt tcttctcttg ctaaaaataa aattttccaa taataattcc tctgttttga
     1441 tttatgaaac aggggaatta ttattaatgg tgaattattt aataatgcaa aatattttta
     1501 tattcaattt agctttttat ttttaattag tataacatgc tatataatat tttaattata
     1561 aagaaatata gaaatatttt ttaataaatt agaaattgga ggagattaca tgagagaggc
     1621 atcatattta caagaacaac tttataaaca taggaagtca ttaaatccta ttgcagagat
     1681 tgggagaaaa gagtataaga cttcaaaata tataagagat tatcttgata aattaggaat
     1741 tgagtatgaa gtttatttag aaacaggtat agtaggtgta atagaaggta aaagacctaa
     1801 aaaagatatt gcttttagag ctgacataga tggtcttcat agtgaagaag gggtaaaaca
     1861 tttatgtggt catgatggac atgctactat attacttgga ttaattgagt atttaaatga
     1921 taataaagaa gacttaaatg ataatatagt atttatattc caaccagcag aagaaggtcc
     1981 tgggggagca aaagctttaa tagatgaagg agtgcttaga aaatacaata ttgatgaaat
     2041 atatgggctt catatatatc cagagttacc agaaggttat gtaggtacaa gagaaggcta
     2101 ttttatggct caaataggag atatcgacat agaaatagtg tctaaaagtg gacatggggc
     2161 tatgccacaa aatggtattg atggtatagt gatagcttca aactttgtaa gctcacttca
     2221 aactatagtg tctagaaata taagcccaat agacaatgca gtgttgacta ttggtagaat
     2281 tgaaggggga gctagaagaa atataatagc tgaaaatata aagatggaag gtactatgag
     2341 atgtttcagc ccagaagtgt atgagaaaat gaagcttaga gttagagaac ttgctagagg
     2401 gtttgaattg gcttataact gtaaggttaa tataaatatt atagatgatt atattgctgt
     2461 taaaaatgac aaggatttgt atcaagagtt tgtagaggct attggaagtg atactgttgt
     2521 agagttagag ccacttatga tatcagaaga tttttcttat tatcaaaaag aagttccagg
     2581 attatttttt atgttgggtt caagaaacga agaaaaagga tttgtaaatg gtcttcataa
     2641 tataaacttt aattttgatg aaaagatatg tgtaaatgca ttgaatgttt attcaaaatt
     2701 attaaaatac aaagaagcaa tagattagga agataaatta taaagattaa aaagcataaa
     2761 tacctaaatt taaaaaaaat gtattaataa atacccctta tatatttgta taaatttatt
     2821 ttaattgaca aaaatacaaa taagaagggg tgattttttt gaaagaaaaa gtacaaagag
     2881 aaaagaagat aactaatggc aagaatacta aaatattgac aaatgatgat ataatgaaat
     2941 atgagatagc ctcagaacta ggtcttatgg ataaagttgg tgaacgtgga tgggcaggac
     3001 ttacagctaa ggaagcagga agaattggag gtatgcttac atcacgcaaa aagaggctta
     3061 aaaagattgt ggaggagcag aataaaaagg atgagtcaat atagtgattt tgcgtttgta
     3121 tatgatgaac ttatgaatga agttgattat aatggttggg ttaaatatat agaagatata
     3181 atagatagtg aaggtgtaaa agtaaaaaat atattggaat tggcatgtgg cacgggtaat
     3241 cttacaattc cattgactaa aaaaaattac gatatagctg gtatagatat atctgatgag
     3301 atgcttagtg tagcaagaga aaaggcagaa aaagaaggag tagaattagt tttacttcaa
     3361 caagatatat cagagcttga ttttgaaata tctgatttgg actgtgtgct ttgtgcttgt
     3421 gatggattta attatataac ttatgatgat gatttggaaa atgtattttc aaagacatat
     3481 gaattattaa agaaagatgg aatattcata tttgatataa gttcttttta taaattagcc
     3541 aacatattgg gaaataacat gtatggagaa aatagagaag atatagcgta tatgtggcaa
     3601 aattactttg atgaagaaga gaatctaatt gaaatggaac ttacattctt tattaaagat
     3661 gaatgtggaa agtttgaaag atttgaagag atacaccaac aaagagcata tactgaagat
     3721 gagatagttg atatgcttga aaaatctgga tttaaggata taaaatatta tggggatttt
     3781 acatttgaca aacccagtga agaaagtcaa agaatcttct ttgtatgtaa aaaataagat
     3841 atttttgact atataagata attattaaaa ataaccatga tataaaaata tagaaaatgc
     3901 agttttatag attaaaagta aagtgatgat actttatgac ttttattttc taataattat
     3961 aaagttctat agataaaaat taaaatataa taactattgc ttagtaaaat taaaataaaa
     4021 tgggttttgc ccaaggttaa tatagataag tacttataga ttaaaattaa ggtagtaata
     4081 taataaaaat aagacaatta acgggacatg gaggaatatt aatgagagat tatgtactaa
     4141 gagcgacatc aggaaatggt caagttagag catttgtcgc aactactaga aatacagtag
     4201 aagaagctag aagattacat gaaactacaa aagtagcaac agcagcactt ggaagaacat
     4261 taacagccac atctataatg ggattaatga tgaaaaatga tagtgataaa ttgactgtaa
     4321 taataaaagg tggaggtcca attggaacta ttatcgccac atcagattct aagggaatgg
     4381 taaaaggata tgtaggaaat ccacaagtag aggttgaaga ctatccaaat ggaaagttaa
     4441 atgtagcagc agcagtaggt acagaaggtg ttgtaaaagt tataaaagac ttaggtctta
     4501 gagaaccata taatgggact tatccattgg taagtggaga aattgcagaa gactttactt
     4561 attattttgc tgtatcagag cagactccat cagtagtagc tttaggtgtt cttacaaaag
     4621 aagatgaagt agagtttgct ggaggattta tagttcaatt aatgccagat gcagaagaag
     4681 agactattgc taagttagag gaaaatgtag ctaaattacc ttctataact aacatgttaa
     4741 aagaaggaaa atcaccagat gatatattaa atattgtttt ggatggtttg gaacctaaaa
     4801 tacttgatac ctgtgaagta gggtttatgt gtgaatgttc aaaagaaaga gtaaaaactg
     4861 ctttggttgc aattggtaaa aaatcattgg ctcagataat agaagaagat aaaaaagcag
     4921 aagttggttg ccagttctgt aataagaagt atatgtatag tgaagaagag ttgcttgaaa
     4981 ttttaaagga aatgtaggct ataaaaatag ttgtattgat ataagtaatg ttatagtgat
     5041 atagtaaagt cgtattgata tagtaattaa agtataattt attaataata gacatattta
     5101 tattaaaaaa taaatttaat aaatttttaa gcttaataaa ttttaagaat taagaagctt
     5161 tctggctgta atatttgcaa tgttgcagtt agaaggcttt tttattttat tatagttcgt
     5221 gtcaaagatt gtatgaaaca ataaaatatt gtaaaataat ttgtatattt agacctgatt
     5281 gacacaaagg tttataagat atttgagttg attatacgaa attctatgaa gtagtaaccc
     5341 tgtgacttta atcatgggag gttcagaaga aggagaaaat tttaaatatg gaaagtaaaa
     5401 cgaagtacat aggaataagt atattaatat tattgatgat atttttaatt gcaaagaatt
     5461 ttaacggaaa acttgaaata cctattcctg agagtaatag tagaaaattt aaatcagcag
     5521 cagtgtttta tcctcaacat caagatgatg aggttttatg gggaggaagt gccattgtag
     5581 atgctataaa acagtgtggt gttgataatg tatatgtggt attagtatca gatggttctg
     5641 gagttaacgt tttcaaagct aacacaaaat ttagaaactt aactaggaag caaaaggaag
     5701 agcttagaaa taatgagttt aaatcagctt tgagagagct tggagtgaaa cctcaaaatg
     5761 tgattatact tgcagatata gataaaaagg aaggcacaca ttatgaatta atggaaaaga
     5821 ctatattgga ttttgaacat aagtttaaag gcaatgtgac ccatatagcc catcactata
     5881 agtatgatga ccatataatg catagaaaga atggggaagt tttaaagaaa cttaaacgtg
     5941 aaggtaagat aaaagatgac ttgtattttg ttaagccaaa atatgtaaag tatataccaa
     6001 gaaatgaaag agctatctat aaagcaatgg attttgatga ctacaataag gttaagagtg
     6061 cttgttatga atataaggtt gtaaattcaa agcgtaaaat gtatggtata ggatatattt
     6121 cggcacatag ttattttgaa catcttctaa atgaccctga acttacgtct gtattaagtg
     6181 aagattaatt tcttataagt agtaagttat gtttgataag ttgattggaa ttttctatag
     6241 ttttgaaggt attggaaatt tagttttatt taaagattta aagatagagg tttttaatgt
     6301 ttaaaatttt aaagttaagt tgaataattg tatagaatta tttgtgaaag aagaaacagt
     6361 gtaaaacata gtatcttatt tatggtttta aaatcatagg caagatactt tttttatgta
     6421 actaatacca ttttgaggtt attaacttaa gaattttata taatttaaca ctcatatata
     6481 atgatgatat ttaggtagaa tagatgcaat tacgcttaaa taaaataact gtatttttta
     6541 taaaaa
//
LOCUS       DACTGW010000198         6457 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.198, whole genome shotgun sequence.
ACCESSION   DACTGW010000198 DACTGW010000000
VERSION     DACTGW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.198"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            176..1150
                     /locus_tag="I9573_003010"
     CDS             176..1150
                     /locus_tag="I9573_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HAT4797608.1"
                     /translation="MNFIGNRYEVVNSDDVLEINKIYKARDVFYRRNVLIKVIKHNNY
                     ICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGIGLDELIL
                     GNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDFGITKANN
                     GVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNSEEKMLQS
                     IDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKAYIEVEDE
                     KEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            1396..2463
                     /locus_tag="I9573_003011"
     CDS             1396..2463
                     /locus_tag="I9573_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAT4797609.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHTGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSEDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            3000..4910
                     /locus_tag="I9573_003012"
     CDS             3000..4910
                     /locus_tag="I9573_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAT4797610.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILSYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(4973..5596)
                     /gene="ytaF"
                     /locus_tag="I9573_003013"
     CDS             complement(4973..5596)
                     /gene="ytaF"
                     /locus_tag="I9573_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HAT4797611.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLELNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            5781..6425
                     /locus_tag="I9573_003014"
     CDS             5781..6425
                     /locus_tag="I9573_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4797612.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQRPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
BASE COUNT         2752 a          620 c         1014 g         2071 t
ORIGIN      
        1 atttcaacaa tacaaaagaa gcgtatctct attttgagac agcctttttt gtaaaaatta
       61 acactattag ttcttgtttt aatattacaa tcattaatat atagaaaata ttcctatata
      121 ttggtataat tgtaaataga tttagttata taaaatgtat gaggaggtga catggttgaa
      181 ttttataggc aatagatatg aggtagtaaa ttctgatgac gttttagaaa ttaacaaaat
      241 ctataaagct agagatgttt tttacaggag aaatgtgtta ataaaagtta taaagcacaa
      301 taattatatt tgtgaagatt ttgtttcaaa tttaattgat gaaagtactg ctttagatga
      361 gataaactct ccttatatac taaaaataat agatgttggg attcactgta cagaagaaac
      421 aacattatat tatgttgtaa gtgaagactt taatggtatc ggactagatg aattaattct
      481 aggtaattat cttcatttag aagcaattgt aaacataatg atacaagttt taaaagcttt
      541 agaaatgata cataggaatc attcatatca tggtagttta aaatcgagta gtataatagt
      601 agatacagaa tacaatataa aaatttgtga ttttggaatt acaaaagcaa ataatggggt
      661 taatacaaga agctatggaa acaggagata tttatgccca catcaactat gtataaatta
      721 tactgataaa gaaagtgatt tttttgcaac aggtcttatt ctatttgaat ctatatttaa
      781 aaaattgcct tttggagaat caaattcaga agaaaaaatg ctacaatcta ttgataaggg
      841 attagattgg aatagtataa aagcaattaa tggaaataca gaacttataa atgttataaa
      901 aaggcttctt ggaagaaata ataagtatac tagagccaat gatattatta ttgatttaag
      961 taaagtaatg tatgaaaaag catatataga agttgaagat gagaaagaag aagaacagaa
     1021 acaagacaaa gttatacaag ttaaaaaaga atataaaagt aaaatgagaa aaaaattaca
     1081 taaaaaactt gttgttgccg gtctagctat agtcatgatt tcaatgatta ttattagttc
     1141 cataatataa aaattgtata ttaagtattt taaaatcaat gtctataaat gatatacata
     1201 gaaattttag tgtagtctta aattatgaaa ggaagtggtt attatgttaa caaaaaataa
     1261 tgaggtatta aacgtctagg agtattttaa caaatagcca cctgctttta cagtttagaa
     1321 taaaaaagct gtggctaaag gtcacagctt tttgctactt tttaaaggca ggagaatatt
     1381 aaaaaggagc aaattatgag caatataaaa ataatagcaa gtaataaatg ttggattgaa
     1441 gatatagcaa ggaaacaact agaagatatt tcaaagttaa atggaatttt aagagtagta
     1501 ggattacctg acttacatac aggtaaaata cctgtaggtt tagcagttga gactaaaaat
     1561 attatttatc cacatataat tggaaatgat ataggatgcg gaatgacttt atttaaaact
     1621 ggggttctaa aaaagaaatt taaaaaagac aaatggatta agtcacttag taagattaaa
     1681 gatttgtccg atatagaaat aaaaaacaca tataaagaag aatgtccaat tttaaattta
     1741 ggaactatag ggagtggaaa tcattttgta gaaattcaat gtataagtga aatttataat
     1801 aaagaacaat ttgaacaatt aaaattttca tctgatgata ttatgatgtt agttcactgt
     1861 gggtcaagaa attatggaga agatatttta aaaaaatttt atgataaaga tggacttgaa
     1921 gttgaaagtg agaaagcaat agattatatt aaaaaacatg ataatgcttt aatgtgggca
     1981 gagcgtaatc gtgaagctat cagtaaaaaa attatgcagt caataggtac atctgaagat
     2041 gttgaaacaa tattctctat aaatcataac tttatagaga aaagggaaga taaatttata
     2101 catagaaaag gtgctgtttc aagtgaaaga ggagctgtga ttataccagg ttcaagaggc
     2161 agtctatcat atatagttat gcctactgaa aatacagaga tatctttata ttctctatca
     2221 catggagcag gtagaaaatg gtctcgttca gtttgtaaat cgcgccttaa aagtaagtat
     2281 agtaaagata ctataaaaca aactaaattt aaaagtcaga ttatatgtaa tgatgtaaat
     2341 ttattatttc aagaagctcc agaggcatat aagaatatag agcagattat tgaaagtttg
     2401 atagaatatg aacttattca agtagtggca actatgaagc cattgattac ttataaagga
     2461 taatttttaa agttaaaaaa gattaaaatt atattataaa tagctaattt tatggaacat
     2521 ttaaaagtgt tttattatta ttagctattt ttttatttta tagagacgat aatttataga
     2581 gctaaattct ttgatatata atgattacaa agttagattg tattggaaac tacacataca
     2641 tgaattgatg acaatataag taattccaaa atataaatga aagtataagc ttattttcac
     2701 tataaaaatt ttcactgtac aaaaacatta ataacattat ttttattgac ataaatatat
     2761 aatttatgga ataatattag aagcactggt ataagtgttt aaataagaat agaaaaggag
     2821 aagtgtttta tgaaaaaaaa tactaacatt aatgagtccg gagttaaact tcttaataaa
     2881 taaggagttt gatatataat aactccttat ttattaagtt gttcgcaaaa taactattat
     2941 caaagtaaat gatagggtga tttaaaagac gatgacttat aaataaagga gaaaaaataa
     3001 tgaatagtaa tacatttgaa agattgcaac taaatgaagt taaagactta ataaaaatac
     3061 attgtgtaag ttcgctagga aaaaatttaa tagataaatt aactcctagt gggaatattg
     3121 gagtagtaag aaggaaatta gcagaaaata aagaagctag aaagataatt gaaaatagta
     3181 atcacatacc acttgaagga ttgtttaatg caggttctac aatagataaa attgaaaaag
     3241 gaatgataat agaacctgtt gaacttgtaa atattgaaga ttttttaaga ggttgtagga
     3301 agatgaaagc ttttatgtta gaaaaagaat tttattcacc tacattaagt tcttatgctt
     3361 taaatataac agaatgtaaa agtatagaag atgagataaa ttactgtata aaatctaata
     3421 aagttgattc taatgctagt aaagaattaa aaaagataag acgaaatata gaaattacag
     3481 aaggaaagat aaaagataga ttaaataaat ttataacatc aactgtaaat aaaaagtata
     3541 tacaagagtt tataataagt aaaagaaatg atagatatgt aataccaatt aaatcttctt
     3601 ataaaaatga agttaatggt actatattag atacatcatc aaaaggaaat actgtattta
     3661 ttgaacctat tagtgtttca aatttaagta cagagttaac tatgctaaaa gctgatgaaa
     3721 ctattgaaga atacaaaata ttatcttatt taacagaact tatatttgag aagataagtc
     3781 agataaaatt aaatatagag atactttcag aatacgatat ggtatttgca aaagctaagt
     3841 atagtcaaaa aataaaagga attactccaa aaataaataa taatggctat ataaaaataa
     3901 ttaaaggtaa acatccactt ctaacaggag atgcagtacc tttagatttt gaaataggca
     3961 aaaattatag aagtcttata ataacaggtc ctaatgcagg aggaaaaact gtaaccctta
     4021 agactgttgg tttactaact ttaatggttc aatgtggatt tgatatttct gcaaaagaag
     4081 gtagtgaatt tagtgtattt gaaaaagttt ttgtagatat aggggataat caaagtattg
     4141 aaaatgcctt aagtactttt tcatcacata taaaaaatat tgcagagatt atgagtttat
     4201 caaataattc tactcttgtt ctgtttgatg aaataggaag tggaactgaa cctaatgaag
     4261 gagctggttt agcaatatca ttattagaag agttttacaa aatgggatgt ataactatag
     4321 catcaactca ttatggagag ataaaaaagt ttgctacatt acatccagaa tttgaaaatg
     4381 ctggtatgat gtttgataaa gaaacgttag aaccattgta taagttaact attggaaaat
     4441 ctgaagatag taatgctctt tttatttcta aaaaaatggg aataaaaaat agagttttag
     4501 atagagctaa agaatatgta attgatagaa actacaattt agatttagtt aaaagaagta
     4561 agctacaatc taatattgaa gataaaaata gttctgaagt cattttaagc tatacagatt
     4621 ataatgtagg ggataaggta aaattactag atgaagatga ttttggaatt gtatataagc
     4681 ctatggacaa gtttaataat gtagaagtat tgtttaatga taattttatt tcagtaaata
     4741 taaaaagatt acaacttagc ataaaagcat cagaattgta tccagaaggg tatgatttag
     4801 atgtattatt tactagtttt aaagaaagaa aattagaaaa agatattcaa agaggttcaa
     4861 aaaaagctct taaaaagatt cagaaagaaa ttagagagaa tagaaaataa atataaaaag
     4921 cacaaaagaa agcaatctaa agagaaaagt ttatctttag attgctttct tattaacata
     4981 tcttcataaa tccaatgact aacaagatta aaccacttaa ccaagataag tttaaattta
     5041 cgtttttaac aaacatattt cctagtaaac aaccaagtaa tactgcaatt atatttgata
     5101 ttaaagaaaa tagaataact tgtatataat tcacacctac aaggccacta ccaaaaccga
     5161 tagctaatcc atctaaactt aaagcaagtg caagtgtaaa tgcttcagca gatttaagca
     5221 ctttagattt acttgtatct aagacatcag gttcgatact tacattaaaa tttaattcaa
     5281 ggtctaacat attaaaatta taattctttg agtgtataga ttttttctta aaataagatt
     5341 taaaaaaact ttcaattagt ctagcacttc ctaataaaaa taaaataaag aaacatatta
     5401 acattgttat attttgaggt aaaaaatctc taactattgt acctgcaaaa agcgatattg
     5461 ctaaaatagc tgaacaaact acgctgataa ttatatttga atataaggga actttaactt
     5521 tatttactcc atatccaaag cttgtcacaa aggcatctat acaaagagaa gtaactaata
     5581 aaattgattc aatcacagta gattaacatc ctttctctta aattttgtac ttatattagt
     5641 tatagtattg taaaaatgga taaattgtta cagataatcc aataaattgt tgataaaatt
     5701 tcaacatcat acaacaccta aataacaaaa gaatcatatc atataatata aaaccaatca
     5761 taataaagca ggtgaaaata atggaacaac atagtaaggt tgggaatttc ggtgatttgt
     5821 tgaaagtttt attagatgaa aaagaattat caatgggaga actaagtaaa aaaagtggaa
     5881 ttgataaatc tactatatct cgtatagcta ataataaaca aaggccaaat ataaatcatt
     5941 tagaaaagat ggcaatacat ttaaatatta acctagaaga actgttgaaa gcttcaggat
     6001 atgaatttag aaacagcaat aatcaaatat ttaatgcaga tagtgatttt agtaactttg
     6061 atgatattct tggatttgca aatttaatca atgataagaa ctttaatgga aatattgaaa
     6121 aagagttaag taagtgtaag ttatatgtcc aaacagatga aggtaaaaaa ttactttttg
     6181 ataattttaa taaaaaaata gattctattg aaaaacaagg tcaatttaca gatagattaa
     6241 gacaaatgta tgctgatttt tgtacagatg gacttacaat aaagaaatat ttattgattg
     6301 gaagtatgct actttacttt gttatatcaa cagatgtgat tccagacttc gtgttcccaa
     6361 taggatttat ggatgattta gtagcattaa atatagttac aaaattactt aaaaatgata
     6421 agtaagtttt agaataatat ttaatatata aataaat
//
LOCUS       DACTGW010000199         6420 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.199, whole genome shotgun sequence.
ACCESSION   DACTGW010000199 DACTGW010000000
VERSION     DACTGW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.199"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            466..1755
                     /locus_tag="I9573_003015"
     CDS             466..1755
                     /locus_tag="I9573_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HAT4797613.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1809..2888
                     /locus_tag="I9573_003016"
     CDS             1809..2888
                     /locus_tag="I9573_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HAT4797614.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2909..4453
                     /locus_tag="I9573_003017"
     CDS             2909..4453
                     /locus_tag="I9573_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAT4797615.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4616..5158)
                     /locus_tag="I9573_003018"
     CDS             complement(4616..5158)
                     /locus_tag="I9573_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HAT4797616.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDNKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5306..5590)
                     /locus_tag="I9573_003019"
     CDS             complement(5306..5590)
                     /locus_tag="I9573_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT4797617.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5653..6417)
                     /locus_tag="I9573_003020"
     CDS             complement(5653..6417)
                     /locus_tag="I9573_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAT4797618.1"
                     /translation="MNNYLKFATYQLIGFTSSIPSLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2223 a          798 c         1127 g         2272 t
ORIGIN      
        1 actaaaaaat gtaagtaatt gactttttaa gttgacaaaa tattgcatat atattatgat
       61 ataaatcata aattaactac aaatcaatat tatatattcg tagaagaaga aagtagcata
      121 aaggaatttt acagagagtt agatttggct gagaactaat attggaattt atgtgaaaag
      181 gactttggag aatactattg taattatgtt agaatagtgg tttaattcgc cttaagaaaa
      241 tgagtgagta aaattaattt ttactaataa gagtggtaac acggatttat attcgtctct
      301 tggatttttc caagaggcgt tttttattat aaacttattt cagaaaattt aaaactatat
      361 attaaatgaa aattataaaa atatgatagt taataaattt ataaattaga aaattgtaaa
      421 atatgctaag gtcagtagtt ttacgattta aatggaggga tgaaaatgga agaagaaaaa
      481 attaactatg ctctggaaca gattccagag aatcaaaaac gtggttgggt agctatgttt
      541 tcagttttag tagcaatagg tgtggattta tcatctgtta ttttaggcgc tgagttggca
      601 cagagtatgc caatgaaaca agcaatattg tcagtaattg ttggttcatt tttttctgca
      661 attttgtata ctacatgctc tttggttggt tcatctacaa gtttatctac atctatgata
      721 acaaagtatg tatttggaga agctggagca aaaatatttt ctctagttat aggggtttct
      781 ctattaggtt ggtttggagt tcaagtaggt ttctttgcac aaaatgcaca gataataata
      841 aaagatatat ttaatttaga tgtcagtatg cagattttaa gcctaatagg tggtctcttg
      901 atgatgagta ctgcaattta tggatataag gccatggaga agttgagtgt gtattcagta
      961 ccatttttat tagttcttat gatgctaact atattcttag cttttagagc aaatggaatt
     1021 tcagttgatg ataatatgaa atctactatg acatttgcag gtggagtttc tctgtcaatg
     1081 agtataataa tagttggtgc aattgtatct cctgatataa gtcgttgggc taaaagtaga
     1141 agagactgtg ctctttcaag ctttttggga atacaatttg gaaatgcttt tatgataata
     1201 gtttcgatag tattagtaaa atgtatggga acttcagata ttatgagaat ttttattaca
     1261 ttaggaattg ctataccagg tataatagta cttacattag cacaatggac tacaaataca
     1321 agtaatgtat attcagcatc attaagtata gctcttgtac ttaagaaggc tcctgagaaa
     1381 gtattaacta tagtccttgg tattatagca acattattgg ctgtatttgg aatatatgaa
     1441 ggttttatag gatttttaaa tttacttgga attgtaattg cacctgttgg tggtgtatat
     1501 acagcagaat attacatagt aaagcaagaa cttaaaggct ttgataaagg agtattatat
     1561 aaaccaatag ttaaacgttc aatagtgtct tggataattg gtatattaat aacttacctt
     1621 tcaacatatg gatttataac tcttactact atagcacctt tagatggttt tatagcaggg
     1681 tttgtagtac aatctattat tggaaaagtg ctttgttcaa caaaaaataa gcaagaaata
     1741 gacaaagcag tataaagaaa tttgtttata aagtataaaa taaattagaa atatgaggag
     1801 aaagtaacat gagattttta acagaagaaa gtgtagataa aatagcagtt ggtgcagcag
     1861 tgcttggaac tggaggtgga ggtgacccat atgtaggtaa attggttgca aaacaagcca
     1921 ttaaaaagta tggtcctgta aaagtaataa gtttagatga acttgatgat gatgctttgg
     1981 tagttccagt atctggtatg ggttctcctg taattacaat agaaaaatta ctttcagaag
     2041 tagagttaac taccccttta gaaataatgg agaaattatt aaatagaaaa gtggatgtta
     2101 taattcctat agaaatagga gggataaact cacttatgcc tatagcagtt gctgctaaaa
     2161 aaggacttcc tatcttagat gcagattcta tgggtagagc tttccctgaa gctcaaatgg
     2221 taacttttta tcttgaagga tatgaggcat ctcctgccgt aatgtctgat gaaaaaggta
     2281 attctgccat cctttatcct gtggatggaa tttggtcaga aagattagct agaactttaa
     2341 ctgtagaaat gggaggaagt tcttctatta gtgactataa tttaagtgga gctcaagtta
     2401 aaaaagctgc tataggtaat actcttacaa tagctgaaac tataggaggg tttttacttg
     2461 aaaataaatc tgattcaaaa ggtgctgttg agaatatact aaaagaattg tgtggataca
     2521 agttatttga aggaaaagta attgatatta agagagagtt gaaaggtgga tttactagag
     2581 gacatgcctt ttttgctggg attaatgagt atgatggaga gtattctata ttattccaaa
     2641 atgagaattt aattgccaaa aaaggtgata caccactttg tattacacca gatttaatag
     2701 cagtattaga tttagaaact ggatttccaa taactacaga gagaataaaa tatggctctc
     2761 gtgttatggt agtagctttt ccttgtaatg aaaagtggag aacagaaaaa ggaattgaga
     2821 cagttggacc tggatacttt ggatatgatg tggaatataa aacagtagaa gaattacaag
     2881 gaaaataaaa gtttaggagg aaattttgat gtatcgtgta ggaattgacg ttggaggaac
     2941 aaatacagat gcttgtatac tggatgggga attaaaagta attcattcag ttaaggtggc
     3001 aactacaaaa gatgttgaaa ctggtgttta caatgcctta aaaaaagtta tagatgaaag
     3061 taagattgac cattcattaa taaaatatgc tatgcttggt acaactcatt gtacaaatgc
     3121 tattgtagaa agaaaaagac ttaataatgt agctatgata agaataggaa agccggccac
     3181 tactgccatt cttccattta tagattggcc agaagactta agagaaaaaa tagaattaga
     3241 ttcaatattg gtatctggtg gatatgagtt tgatggtaga aagataaatg aactttgtaa
     3301 agatgaagtt gtagagtttt gcaatagaat aaaaggcaaa gtggaaagtg ttgcaataag
     3361 tggagtattt gctccagtaa ataaacaaca agaagaagaa gttgcaaaat gggttagaga
     3421 aattttaggg gatattccta tatctttatc aaatgaaata ggtaatatag gtatattaga
     3481 aagagaaaat ggagctattt taaattctgc attgtctcaa gtaggtaaag ctgtatcttt
     3541 aggatttgaa aaagcactta aagacttagg tatagatgct caaatatatt tcagtcaaaa
     3601 tgatggaaca ttaatgaatt tagagtatac aatgaaatac ccaattttta caattggatg
     3661 tggaataaca aattctataa gaggagcttc cttcttatca aaagtaaaaa atgcaatagt
     3721 attagatgtt ggtggaacta caagcgactt gggtgtacta tataatggat tccctagaga
     3781 atcatctatt ccagttacaa ttggaggagt acatacaaac tttagaatgc cagatgtttt
     3841 aactattggt cttgctggtg gtacatgtgt aaaaggcgaa aaagataata taaagatagg
     3901 tcctgaaagt gtgggatata gaataactga agatgcaata atatttggtg gagacactct
     3961 aactttatca gatgttgtta caaaattaga tatggcgttt gaagatagaa aaagcaatgt
     4021 tgaaaactta gataaagagt attgtaatga ggtatataag aaggttattt taaaattaga
     4081 agatgctgta gaccaaatga agactagtca agatgatgtt gaattggttt taactggtgg
     4141 aggaagtata attgttcctg aaaaactaaa aggcataagc aatgttataa gacctcctca
     4201 ttatactgct gcaaatgcta ttggtgcagc tttaggacaa ataagtggag atgtagaaaa
     4261 agtatacagt ttagataaaa tgtcgagaga agatgccata gaagatgcaa aaaaagaggc
     4321 tgtaaataaa gctctaaaag ctggtgctaa agaagatact atagaagtat taaatataga
     4381 agatgttcca cttgcatacc ttcctggtaa tgcacttctt ataaaagtaa aagttgttgg
     4441 tgatttaatt taaagaataa atataagtat ttccagaatt atataaatat ttctggactc
     4501 atttttaaac ttattttatc aataaaaaca gcataacttt tgactatatt cttataagtt
     4561 tccgaacagc cgatatatac ctattttttg aggtttatcg gctgtttgtt atcttttatc
     4621 gcattccaac tatttttctt accgtatttg ctgtacgaat tgtaatctta tcacttacat
     4681 ttgaactggc agtctttgac caccaatttg tctttttata atctttacga ctaaaagacc
     4741 aaaagatagc atttttataa tgatatactt tttcatactc ttctttagga tttccaacct
     4801 catcataaaa ctcgtcataa gataagtcag gaaacataaa aataagatta tcatatattt
     4861 ctttgttgtt atctccccac caattaggag cattatttag tatatctttt aactcctctt
     4921 gtgaaataat aaaaactggt atttccaatt caaactgttt ttttatcatt aatcttattg
     4981 tatttgagat aaccttgttg tcatctacat cacttaaaaa gacaacatta ccactattaa
     5041 ggtatgtaga aacttctgaa taaccaagtt ctacaaagct tgcttttaat tcagacataa
     5101 ttatcttgtt cttaccactt atattgatac ctcttaataa tgcgatataa cttttcatat
     5161 aataagttca cctctaaaca ttcacatttt tctatgtagt acaaactgga atttgcatct
     5221 tttataattc tttcaaaact tcttcatatg taattttttt aatataaaaa aatcggtgct
     5281 taatttgtac cgatttttat ttttcttagt aaagaactcg tttttttgct tctgctatag
     5341 ctctagcagc actttttgct tgcattcttt tttcttcttc ttttttaaat tcatttattg
     5401 tttcatttag attcttatca tgttcatttt tttctttatc ttcgttttct ctgtatacta
     5461 atatataatc tattattgat tcgtttatta tctgagatat acttttattt tcattaattc
     5521 ctattctctt aacttcctta agtagagtct catcaatatg aaccgttgtt tgctttgtat
     5581 tttttgccac cttaatcacc tcttaaaaaa aattctaaca ttaaaatttt ttttagtcaa
     5641 taaattttaa tattaaaatt ctttaaatgt tttatttgat tttctaaaat tttctattgt
     5701 atttatcatc ttatttttta tattttcacc tagtacagaa gactttagtc taggattttc
     5761 tatatggtca taaattggtt ctaaaaattc gacttcgaca ggaacagagt taattttacc
     5821 aataggttgg tatccttcat aagtatcttt tgaatttttt attacgagtg gaacaatagg
     5881 gcattttgct ttatatgcta tctttaaagc tccacttcta aactccgaaa ccaatgaatt
     5941 tggttcttta atccatgtta aatctccttc tggaaacact gccatacttt gacctgtaag
     6001 tatattttga gaggcttggg ctatactctt tataccctca cgattgtttt ttctgtttac
     6061 atatacacat cttagtaatt ttgcccattc tttaaatata ggtatatttc tccaaactgg
     6121 ttcatctgca attacacatc caattggtct ttctacactt gctgttagta tgaagctgtc
     6181 aagcatacta gaatgattta caacaaatag aagtggttct tttggcagtg tttccttacc
     6241 aattatatta agctctatgt tcactatatc taaagatttt ttagcttgtt tttgcataaa
     6301 ttcaaatttt tcctttaagc taaatttatc aggattcttt ttaattttaa taaggctagg
     6361 tatagaagat gtaaaaccta taagttgata tgtagcaaat tttaagtaat tattcataaa
//
LOCUS       DACTGW010000200         6420 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.200, whole genome shotgun sequence.
ACCESSION   DACTGW010000200 DACTGW010000000
VERSION     DACTGW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.200"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            351..1358
                     /gene="asrA"
                     /locus_tag="I9573_003021"
     CDS             351..1358
                     /gene="asrA"
                     /locus_tag="I9573_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HAT4797619.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEIIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            1359..2150
                     /gene="asrB"
                     /locus_tag="I9573_003022"
     CDS             1359..2150
                     /gene="asrB"
                     /locus_tag="I9573_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HAT4797620.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            2163..3134
                     /gene="asrC"
                     /locus_tag="I9573_003023"
     CDS             2163..3134
                     /gene="asrC"
                     /locus_tag="I9573_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HAT4797621.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            3191..3967
                     /locus_tag="I9573_003024"
     CDS             3191..3967
                     /locus_tag="I9573_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAT4797622.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            4238..6256
                     /locus_tag="I9573_003025"
     CDS             4238..6256
                     /locus_tag="I9573_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAT4797623.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLIMERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
BASE COUNT         2599 a          554 c         1150 g         2117 t
ORIGIN      
        1 taaaaatatt attaagagaa tctatttata gattcttttt tattgtctaa tttattaatt
       61 tttattttac cctattgaat tgtataaaaa agcaaataag attctttatt attcaaaaaa
      121 ttgagtagat aatagtttta ttgtatttaa tgattgtatc taagtgatta gatttgaaat
      181 cgaataatat agataataaa aaaatattaa cagagattag taaactctat taatattttt
      241 ttattatttt tgaaactgtg ataattatca cagaagcata gaaaataaag tgttaagatt
      301 taaacaagat agcaagtaag taaaactaaa atataagaaa ggtgataaaa atgaaactaa
      361 aattagattc atctaagttc aatgaaggtc tgaattatct aaaaaaagac tacaagatat
      421 ttgcaccagt tttaatccca tttaaaggta ctttttcaga tactgatgtt ataagataca
      481 aggaggtaaa cgcttttgaa gaaatggaat ttgctaagaa agcaaacttt tcaccaaaag
      541 aagttgtact tcccataaat caagtattgt tttattttac tgaaaaagag tttaaggaaa
      601 gcgatttaga caacaagaaa attttaattt tcttaagagc ttgtgacata aatggtataa
      661 aaagacttga tgaaatatat ctaaataatg gtgaagaaaa agattatttt tataaaaata
      721 tcagagataa agtaaagttt gcacttgtag ggtgtaagga aagttttaga aactgttttt
      781 gtgtgagcat ggattctaac aaaactgata attattcaat tggattaaat atagaaggag
      841 atacactgta cttagatata aaggatagtg aatttgaagt atttaatgga gaagcaactg
      901 aatttaatgt tgattatgta actgataatt taatatctgt agatgtgcca gagaatattg
      961 attcaaatga aattatagga aattcaatat gggatgaata tgatacaaga tgtataggat
     1021 gtggtagatg taattttgta tgtccaacat gttcatgttt tacaatgcag gatattttct
     1081 ataaggaaaa cgaaaatgta ggtgagagaa gaagggtttg ggcttcttgt caagtagatg
     1141 gatatacaga tatagcaggt ggaaattctt ttagaaaaaa acaaggtgaa agaatgagat
     1201 ttaaaactat gcataaaata catgatttta aaaaaagatt cggatatcat atgtgtgttg
     1261 gttgtggaag atgtgatgat gcctgtccgc aatacatatc attttcaaag tgtatagaaa
     1321 aaataaatga tttagtaatt agtaaggagg aagtataaat gaacccatat ataccagtaa
     1381 gtgcaaaaat actagatata gttaaacata ctgataaaga atggactttt agagtaaact
     1441 gcaatacaaa aggtgtattg cctggaaagt tttatgagat atctatacct aaatacggtg
     1501 aaagtccaat atcagtatca ggatatggag aggattatat tgactttaca ataagaaatg
     1561 taggaaaagt aactagcgag ttgtttaatt ataaagaagg agattctttt tttataagag
     1621 gtccttatgg aaatggattt gatgtatccc tttatgaagg aagagaaatt gtagtagtgg
     1681 caggaggaag tgcattagcc cctgtaagag gaatagttga atatttttat aataataaag
     1741 aaaaatgcga aaagtttaaa ttaatagtag gatttaaatc accaaaagat attttatttg
     1801 cagatgattt aaagagatgg agtaaaaaac ttgatatttt agttactgta gatggagcag
     1861 aggaaggata tagtggtaat gtaggattag taacaaaata cataccagaa ttggatatga
     1921 aagatataaa caatacttct atagttgtag ttggacctcc aatgatgatg aaatttacag
     1981 tagaagaatt tttaaaaaga gatttagatg aaaagaacat ttgggtttct tatgaaagaa
     2041 aaatgtgttg tggaataggt aaatgtggac attgtaaaat ggatgatact tacatatgtt
     2101 tagatggacc agtatttgat tattcatatg ctaaaaatct tattgattag ggggcgaagt
     2161 ttgtgataag agatttaaat accaaaaaag taatgaaaaa tgcttataga attacaaaaa
     2221 caaaatatga gacttcttta agagttagag tacctggtgg tcttattgac cctgagagtt
     2281 tagctgtagt ttctaaaatt gcaagtgagt acggaaatgg acaggtgcac ataactacaa
     2341 gacaggggtt tgaaatatta ggaataaata tggaagatat ggaagaaata aataaaatta
     2401 tacaacctgt tattgaaaaa atgaatataa atcaaagtga gaaaaacgca ggttatccag
     2461 cagcaggtac aagaaatata gcagcttgta taggaaataa agtctgtcca aaggctcaat
     2521 ataatacaac tgaatttgct aaaagaatag aaaaagctat atttccaaat gatttacatt
     2581 ttaaagtggc tttaactggt tgtccaaatg attgtataaa agctagaatg aatgactttg
     2641 gaattattgg tatggcttta ccaatatatg aaaaagatag atgtgtaaat tgtggagcat
     2701 gtgtaaaaaa atgttctaaa atatctgttg gagcattaaa aacagaaaat aataaggtgg
     2761 taagagataa agataagtgt ataggatgtg gagagtgtgt actaaattgc ccaacaaatg
     2821 catggactag agatgagaaa aaatactata gattagctat aatgggtaga acaggaaaga
     2881 aaaaccctag actagcagaa gattttcttt tatgggtaga tgaggacagc ataataaaga
     2941 taatacttaa tacttataga tatgtagaaa agtatataga taaagatgca ccaggaggaa
     3001 aagaacatat aggatatata attgatagaa caggatttat ggaatttaaa aaatgggcac
     3061 ttgaaggtgt agagcttcca gaaataacta aaatgtacga aaacatatac tggagtggaa
     3121 ttaaatatct ataaattgag taaaacatat atttgaataa attaaaattt aatagaagga
     3181 agagggaatt atgcataaag aaactttgga taaattaact aatgcagcta taaataagat
     3241 aaatttatta aacacaagta aagtaaaata tttggtatct tctgcgtttg caggacttta
     3301 tgtaggtata ggtatacttt tgatatttac tataggagga cttttgactg atgcaggttc
     3361 acctatgact aagatagtaa tggggttatc atttgcaata gcactaagtt tggttataat
     3421 gacgggaaca gagttattta ctggaaacaa tatggttatg tctgctggta tgttaaataa
     3481 aggtgtaagt ataaaagata cttctaaaat atgggcatac agttgggtag gtaatttaat
     3541 cggagctttg gttttaggtc ttatatttgt aggaactggt ctagtagaca aaggacctgt
     3601 agctgaattt tttgcaaata cagcagctag taaagcatcc atgccattta cagctctttt
     3661 ctttagaggg attttatgta atattctagt atgtgtatca gtgttgtgtt cgtttagaac
     3721 taatagtgat actgcaaaaa taattatgat attcttatgt ttatttgctt ttataacatc
     3781 aggttttgag catagtgtag caaatatgac aatttatagt gtttcattat tttcaccaac
     3841 aataagtaca gttactattg gaggagctat ttataattta gtagctgtaa cacttggaaa
     3901 tattgttgga ggagctttat ttatgggtct tggaacttat atattaggta aagaaaagct
     3961 taattaaatt tattgtataa attataactc tcttgataaa tacattttta gtattattga
     4021 gagagttatt tttatatttt tgaaaaaata tttgaaacaa aaaaaatatg ggagcatacg
     4081 aaaatatatc ggttatatat aaaaaaaata cgtttatata tgaattatat agttctaaat
     4141 ataaaaaaag ttaaagtcct accgtaaaaa agcgcatttt gaataagata acaaatatga
     4201 taaaattggt cttgttgaaa tttttagggg ggatatgatg acaaagaaac tagtaatatc
     4261 aaaaacaaag ttgtttattt ttataatagc atttatttct atatttatat atttatttgg
     4321 aagtaaaaat acacttatag gtgtaggtat agtaacagct atgctaacac tcttggaaag
     4381 agatttaact atttctccaa ttaaaaattt actgaagtat ttagcaataa atataatctt
     4441 aggaatacta tcattttttg cagtacaaaa tatgtattta ggagttttat taaattttat
     4501 agcattattt ataatagggt atgtatttag ttatgactta aagagagcag tatatgtacc
     4561 ttttggtcta atgtatatat ttatggtaag tatacctgta ggaaaaagtg aatttcctat
     4621 gagattgtcg gctttagctg ttggagcagt tataataatg attgctcaat ttgttatgaa
     4681 tagaaatagg atgaaaaatg tcggagataa agaactgata tctatatgtg atgaattatt
     4741 agaaaaaatt agtcttttaa aaaatactat taatgatgat agttctataa ttaaatctag
     4801 tatgagaaaa atagatagct gtaattatag aataaattct atatctaaaa atttgaaaat
     4861 ggttattttt gataatagaa aagatgactt ttatatcagt attagaggta tagacataat
     4921 gaatatttta ttttcattag aaagaataag tttaatcatg gaaagataca aaaaggatag
     4981 taaagaattt gaagatgaag atataaaaaa tattttagaa gaaagtagca taaatagtaa
     5041 atcagatgtt ctagttgtag caacgaaaga aataaactat attaagatgt gtttagaaaa
     5101 taaagatacc ataactgata aagagatatt aggatttaga gattatgtta ttgctcagga
     5161 tactaaaaat ataaatttaa aagaaattta tagtgtttta gaaaatttat atgagttttt
     5221 agtagattat aaaaaagtaa aatcaagaga cgaaaagaaa gctgaaagaa aatcaaagat
     5281 acctcatgaa tttaaacgac tatcaatata taagaaaaac tttaatttaa attctataag
     5341 attttcttat gcagtaaaga ttgctttagc tacagctgta gcgggtttta ttatggatta
     5401 tttccattta agagatggaa gatggataat gttaactgtc ttttcattaa cacaacctta
     5461 cgcagaaaat tgtatacaaa ggtctagaaa aagaatagaa ggtacattta taggagctgt
     5521 aatatttatt gtattatttt caataataaa agatagtaca ttaaggtcgt taatagttct
     5581 tttagcaggg tatataaact cttatgtagt tgattataga aaattaatgg tatgtgtaac
     5641 tgtttcagct cttggttcag cagtagttat gggagaccca aatgttttaa ccataagcag
     5701 gatttcatat gtagcattag gagcaattat tgcattaata gtaaataagt ttatattacc
     5761 atatgatgct aaaacaggct atcaacatgt tatagaaatg tataaaggaa tagttaaaaa
     5821 tataattgat gaagtcaata agtctataga aaatgtagct gatgtttact atataaaaaa
     5881 tttattattg ataccatcac ttattgaaga tagacttatg ttgataaatg ctatatataa
     5941 agataaacat caagaggatt ttttagaaaa tcagaaatta ctaataagta atatgtataa
     6001 tctatatata aatgttaata aaaataaaat aaaagatgaa gatgttgaaa aaatattgag
     6061 agatactaat tatatttcaa attacaatgt agataagtat gatgaaggaa gaagtgtaat
     6121 tttagagagt attgtaaata ctaaaagtct aggtgacaag ataatatgtc taaacttact
     6181 gcaaactcta aatggtgtca aagaaatgta tagaataagt aatataacta aagtttcaat
     6241 aaaagaagca gcttagatat taaattatag aatagccata tatttaaaat aaagtatatg
     6301 gctatttgtt ttataaaata ctaaaagaaa ataatatata aaaaacaaaa aacatatatt
     6361 tatgaatttt atatgaaaat agttaaaata ttgatttttt tcattggttg aaaaaacaat
//
LOCUS       DACTGW010000201         6385 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.201, whole genome shotgun sequence.
ACCESSION   DACTGW010000201 DACTGW010000000
VERSION     DACTGW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.201"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..172
                     /locus_tag="I9573_003026"
     CDS             <1..172
                     /locus_tag="I9573_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT4797624.1"
                     /translation="ASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINIL
                     KEGLRLITIQNK"
     gene            290..832
                     /locus_tag="I9573_003027"
                     /pseudo
     CDS             290..832
                     /locus_tag="I9573_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            977..1639
                     /locus_tag="I9573_003028"
     CDS             977..1639
                     /locus_tag="I9573_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4797625.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            1639..2820
                     /locus_tag="I9573_003029"
     CDS             1639..2820
                     /locus_tag="I9573_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4797626.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(3001..5661)
                     /locus_tag="I9573_003030"
     CDS             complement(3001..5661)
                     /locus_tag="I9573_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797627.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5658..6341)
                     /locus_tag="I9573_003031"
     CDS             complement(5658..6341)
                     /locus_tag="I9573_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797628.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
BASE COUNT         2226 a          995 c          908 g         2256 t
ORIGIN      
        1 tgcaagttca caaatatgca ataaatgtgg gtataaaaac gaggaagtta agaacttaaa
       61 tataagagaa tggatttgtc ctagttgtaa tgaaactcac gatagagata taaatgcaag
      121 tataaatata ttaaaagaag gattaagact aataacgatt caaaataaat aacttatgta
      181 agaaccgtag gaactacggg gatggcctgt ggagaattag taagacatat tttagtatgc
      241 aaaattctat gaagcaggaa ccctgtgact ttagtcatgg gaggttcaga atacaactat
      301 gaatgagttg gcaaaagaag ttgaatatac taaacgcacc atatacaagt acttttcatg
      361 taaagaagat ttattctttg ctgttgtctt aagaggatat aaaagacttt gggataatgt
      421 taaaatagaa agtgcaaaag gcaaaacagg gtttgaaaaa ataaaacttt catactttgc
      481 ttttcataaa ttttactctg tagaaccttc tctattatgc cttatgggaa tgattggtat
      541 tgtaaactct gaaaaatcgg atactgatat gctgtttaaa gaaaagtttt tttcttttaa
      601 taaatttatg tttgatgaaa tacaaggaat gtttgaaatt ggaaaaaatg atagaagtat
      661 taggcatgat attgaaatac ctactctcat gtattcatca atatttacac ttacaggatt
      721 ttttaattta ctatctgtaa caggaaagtc atacctgaac aactttaata tagatgagga
      781 aaaatttatt gaaacaactc ttgctctttt aatagattca attaaagcat aaacctaagt
      841 ttataaatta aagtttatat acttaaatga taattttttc tatctctaac ttctatctct
      901 aacataaaaa atattaaaca taaaactaat aacttagtta atgaaataac taaattactg
      961 aaaggactaa aagtaaatgg ctaaaatact tcttttagaa gatgatgata caattacatt
     1021 tggaataaaa acttcccttg aaaagaaaaa tcacactgta tgttgttata atacgattgc
     1081 tgaagctaaa aaaaattttg ataaaactat tgatttaatt ctgcttgatt taaatctacc
     1141 tgatggtatt ggatacgagt tttgccaata tgttaaaagt atagaagata ctccagtgat
     1201 atttttgaca gtccgtgatg atgaaaaaga cattatacgt ggattagata tgggtgcaga
     1261 tgactatgtg gttaaaccat ttctgctctc agtacttcaa tccagaatta atgcagttct
     1321 tagaagaact cattcaaata ctaacaatac tattacatgt ggaaatataa gcttgaataa
     1381 agttgaagtc aaagcatatt tagataatgt tgaagtactt ttgacagcag gagaatataa
     1441 acttcttcta agtcttatgg aaaataaaaa tagagctatg acccgtacaa agttactaga
     1501 aatgctctgg gatgtagatg gtaatttcgt aaatgataac actcttactg ttacaataaa
     1561 gcgtcttaga gaaaaattaa aaaatccacc ttgtataaaa acattaagag gcataggata
     1621 tagaatggag gacagctagt gaaaactcca agaaatatca acagtacagt tgtttttata
     1681 atattctgct taattataac ttttttatta agtgtaattc taacaactat aacttctttt
     1741 aatgaataca atgtactgtc taaggctctt ggtgcatcat atgagtacaa tcctaagtct
     1801 acagaaagta tactaaaatc actgaaaaat tctagtgata aggattttaa aacaggaaat
     1861 gaaattttat caaaacatgg atattctaaa agtacatttt ggagtaaaaa ctttttttat
     1921 tttctagtga tatcgtttgg tataatttca acaatggcta cagttgtata tataatcaaa
     1981 catagaacaa ataagcaaaa actgagtaga ataaacaatc ttactcaata cttagaagaa
     2041 gtgaatttag gaaaagagtc tgtactttta cgatgtgaag atgaattttc tcaccttgaa
     2101 gatgagatat ataaaacagt tggggaactt cgttgttcaa aagaatttgc tttaaaagaa
     2161 agacaaactc tttcagataa tcttgcagat attgctcatc aacttaagac tccaattact
     2221 tctatgtcac ttatggcaca attactttca gaaaattgtc cagatgaaga aatagattat
     2281 attaacagac ttacaaatca aatctcaaga cttgagagac tagtttcatc tttacttaca
     2341 ttatcaaaat tggatgcaag tactttaata tttgaaagca aacttataga tgtacattct
     2401 cttcttactt atgcaattga gcctatagaa ggttctttaa gagaaaaaca tcagacattt
     2461 actatcgagg cttctccttc ggtagaattt gaaatcgata ttaattggac tttagaagct
     2521 cttttaaata ttcttaaaaa ttgtagtgag catatagaag atggtggata tatagttgct
     2581 tattatagtc aaaatcctct atatgttgag attatcatag aagataatgg taaagggttt
     2641 gctcaggaag aacttccata tatttttaat agattttatc gtggtaaaaa tgctagtaag
     2701 gacagtattg gtatagggct tgctctatca aagtctatta tagaacgtca aaatggtact
     2761 ctacatgcag aaaattgcca taatggtggt gcaagattca taattaaatt ttacacataa
     2821 aatgctggat tataggtaaa atacctataa tccagctata tctatattct atgttatcta
     2881 ttgttttagt aatatacttc tatttacatt taaggtttta ttaaacaaac aaattaacta
     2941 tccctatact tgtaagtagt cttaaatcga aacctttaaa taagttcaaa tagcttttgt
     3001 ttatattgtt tcatttttta tagattctac tatattttca ttcttcaact gcttgtatcc
     3061 tgtcatatat gatgctatta caataaaaag tatcaatata gtaaaaccta ttattgtaga
     3121 tataggtgca aatggtaaat actctataaa atatatttca tttatacgta gaaaagcata
     3181 cactattcct atttgcacag gaatactaag tagtaatggt gtaattccta agaacaaacc
     3241 ttccaaaaca agcatctttt ttatgccttc tggagataat ccaacagaac gcaacatagc
     3301 aaactcttgt cgtctacttc tagtatttcc tgacactgta gcaagtacat ttgataatcc
     3361 aatcatagcc aataaacctg taataaagcc tactattaaa ttcatggtct ttattccact
     3421 agccttttgt ttttcacttt ctaaaacatc ctcaatacta taatctcctg aaccatagtc
     3481 tttattataa atggactcta atttttctct tactctagga atgtcctcac gattatttac
     3541 tttaaatact cctatcagcc tatttgaacg taacctactt acttcatcat aatttttaga
     3601 tatttcctgc accatagaca ttggcactat ttgcattaat gtatattttg aactagtatc
     3661 tactgtttta ggaagtgtgc ttgtaatagc tcccactttc atgttgtaag tataatcccc
     3721 tttatcttcg tcatatactt tttcatttaa tttaatgtta tctcctattg aaagatttaa
     3781 ataatctata tatactttat ttctacgtgt actacgatta atatcttcct ccttattata
     3841 tacaattgag gttggatttt ttgtatcata aaataacttt gggtctacag aaatttgcct
     3901 acaatattct gtaaaactct tatcatcaag cccaattata tttgaaatta tacgaaactt
     3961 gccatcacgt tctataggag agtatttttt agatgaaact atacttttaa atcctccatt
     4021 tttcgcaagt tcttttgatg catctttttc agtcagccaa gtggaagcag acattttttg
     4081 ataatatagt gaacttttta caccttctgt attatctaat ttttcaatga aagcagactc
     4141 tacaggttca ctattttcca aataaactgc tatatctctt tggtccttat aattatcact
     4201 tccataaaca gcttttgctc cttcttgatt tgcaattata tgcaaaaaac atgtaagtaa
     4261 taaaaaagat aatgttaaag atattgttgc agtacgataa gattttttac gagcacgtag
     4321 tgcgtttgat gcaagttctc cttctattcc aaatatcttt cctaatgaat atttatgcga
     4381 ctttttgatt ttaacactat ctccttgtcg tattgcttct ataggagata tctttgatac
     4441 ttttcgtgct ggtataagtg cagaaatcca gactgttact attgtaagta aaactgctgg
     4501 cagaaaagca ggaataccat aagtaaatac cacttctgga acatcagtat gataattaac
     4561 tgaattaata taaactataa gcctattaca caataaccat cctaaaaata aacctattgg
     4621 caatggtata attgtaagca aaaaaccttc aaacactaca gacattttaa tctgttttgg
     4681 tgatgcacca acactagata atatccctaa ttgagtaagt ctagcatttg cagatagtga
     4741 aaacgcgttg taaatcacca ttacaaacac agctactgta aaaattgcta ttgtaagata
     4801 cataatagga gttgaaaatt ttgatatact gctcatagat gctttctgtt ctggggaaaa
     4861 gactaacatt tttaaaagat aattactatt atatttaaga ttgtattttc catattcatt
     4921 agtcttccac cccaatgatt tagcaagttt tggaagttcc ttgtatgtgt ctcttatatt
     4981 tttaaatcgc aagtacacag ttatactatc attaggtttt atacttgttc tatcaagata
     5041 tccaagagct gtatatgcag gtattactga tgaagtggaa acatctacct ttcctactat
     5101 tgtcaatgta gtctctgaaa tttttttaaa actttcccct ttcgattttt gagctatggg
     5161 ttcaagaggc tttccattta ggattctatc tcctattgga agagtaattt tatccccaat
     5221 ttttacactt ggattattct caaaatattg ttttgataat gcaatttcat tagcattatt
     5281 tggtcgacga ccctctatta tagtatcctt ttctggcata gaattccaat actctgatgt
     5341 agcatttcta taaattatat aatctctttt ctcatcatta attttgccta cataccattg
     5401 tccttttacc attacatcat caactgaaga aaaattttca attaatggta agtcacttcc
     5461 ataagtatag tcaaacaatt caccatgcca gttaccttct ttctcaattg aaagtcttat
     5521 aaaatctgtc cacatggtat acaacagtcc acacaaagaa gacatcatgg ttgtagtaag
     5581 taatatagct aacattatag ctatactact acgtttattg cgtcgtacaa agtctaaggt
     5641 gtattcttga agaatattca tttcttcacc accttttcag agacaatcac tccatcttcc
     5701 atagttacaa tacgatttgc ctctagtgct attttctcat catgagtaat aagaagtatt
     5761 gtctgattaa atcgtttatt tgataagttt aacaagtcta caatttcttc tgaatttttt
     5821 ctatcaagat taccagttgg ctcatctgct aataaaatag ctggtctata gattaaactt
     5881 ctagctatag ataccctttg ttgttgtcca cctgaaagtt gacttggaag atggtttaat
     5941 ctatcagata atcccaatat agatacaatc tcagagaatc tatcctcatc cacctttctt
     6001 ttatcaagta acatgggtaa taatatattt tttctaacat caagggtagg tataagatta
     6061 taaaattgat aaataagacc tacttttcta cgtctaaaaa ctgcaagagc ttcttccttt
     6121 aaacttgata tttctgtatc ttcaacataa atctttccag atgtaggtct atctactccc
     6181 ccaagcagat gtaacaatgt agatttgcca gaaccagatg ctcctacaat ggatacaaaa
     6241 tcaccttttt gaactgataa atttacatta ttaagggctg tcactctggt ttgatttgaa
     6301 ccatatattt ttgtgagatt ttcacatttt aatatttcca tatacaatca tctcctttat
     6361 ggttaaattt tagcaaccta aaatg
//
LOCUS       DACTGW010000202         6295 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.202, whole genome shotgun sequence.
ACCESSION   DACTGW010000202 DACTGW010000000
VERSION     DACTGW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.202"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..497)
                     /locus_tag="I9573_003032"
     CDS             complement(<1..497)
                     /locus_tag="I9573_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HAT4797629.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFYFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTL"
     gene            complement(527..1636)
                     /gene="ugpC"
                     /locus_tag="I9573_003033"
     CDS             complement(527..1636)
                     /gene="ugpC"
                     /locus_tag="I9573_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAT4797630.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDKIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            1895..2632
                     /gene="cas6"
                     /locus_tag="I9573_003034"
     CDS             1895..2632
                     /gene="cas6"
                     /locus_tag="I9573_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HAT4797631.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            2637..4112
                     /locus_tag="I9573_003035"
     CDS             2637..4112
                     /locus_tag="I9573_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797632.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKKMIDNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            4132..5145
                     /locus_tag="I9573_003036"
     CDS             4132..5145
                     /locus_tag="I9573_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HAT4797633.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIIFGKKGNIYPSIKQNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEKEENEVLR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDKLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            5161..5967
                     /gene="cas5"
                     /locus_tag="I9573_003037"
     CDS             5161..5967
                     /gene="cas5"
                     /locus_tag="I9573_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HAT4797634.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVMPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCNVCIDTKEVFTWL"
     gene            5994..>6295
                     /locus_tag="I9573_003038"
     CDS             5994..>6295
                     /locus_tag="I9573_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAT4797635.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKK"
BASE COUNT         2545 a          632 c         1000 g         2118 t
ORIGIN      
        1 tctaaagttt tatatctaat aaggtcaaac aaatttaaaa acttattttc agcattgtct
       61 tttataatat gaggtagtaa atcagaacat aactgtaatc taaaataaaa atcagcttta
      121 ctatctaatt caccaagata aaaaatataa gtactttgat aagtttcagt ttcaattata
      181 ttttgtgtag ttacattaat atctaaatta tatttagaat ttactataaa aaaaccattg
      241 tgttgtatta tatttatatc ttcaaataaa ttataaaaaa tattttttag gtattcaaag
      301 tctatatgag attggtaaat taaataaaag ttgcctttta aattatcaag attattttta
      361 tctagaaaat caataacata aatagcatta ttgaaagtaa ctaaatggct actagcatct
      421 aacttttggt tttcataggg atagatattt atttttttat taagctgtct ttctaaaaaa
      481 tcttttaact cttccataaa gtctccttaa aaataagatt atattcttat cttatagcta
      541 aagtagtttc tttgtcaaat atgtgtactc tactattgtc aactgaaagt gttaagtcat
      601 caccaacatt aattctgttt ttactgtcaa atctaattgt tatcgtattt tcgcctagtt
      661 ttaagtgagc ataagtttct gctcccataa gttcttttat ttcaattctt gatttaaaac
      721 aagtatctgg attagattta ataaaagtat cttctacatg aatatcttct ggtctgatac
      781 ctataacaac ttcattatta atataattgt cttttattaa atcatagtgt ttttctttat
      841 tcaatttaag cttaatatgt tcattttgtg cataaatttt atcattttct tctattaata
      901 ttacatcaat aaaattcatc tgtggtgctc caataaatcc tgcaacaaat atattagctg
      961 gataatcgta tacatcttgt ggagttgcaa tctgctgaac tactccatcc ttcattacta
     1021 cgattctatc agccatagtc atagcttcaa cttggtcgtg tgttacatag ataaatgtag
     1081 ttccaagctg ttggtgtagc ttagttatct ctgttctcat agcagttcta agttttgcat
     1141 ctaagttgga tagaggttca tccattaaga ataccttagg atttctaact attgcacgtc
     1201 ctaaggcaac cctttgtctt tgtcctccag ataatgtttt aggtttttta tttaaaagag
     1261 gtagtaagtc taatatttta gcagcttctt taactttttt gtctatttca tctttaggca
     1321 attttctaag tttaagtgca aatgccatgt tttcgtaaac agataagtgg ggatataaag
     1381 catagttttg aaaaaccata gctatatctc tatcttttgg ttctatatca ttgacaagtt
     1441 tatctccaat gtatagttct ccttcagaga tttcttcaag accagcaatc attctaagtg
     1501 tagtcgattt accacaacca gatggtccta ctaaaacgat aaattcttta tctttaatgt
     1561 ctatatttat attttttact gcgttaaatc catttgagta aagttttgat atatttttta
     1621 aaattacttc tgacataata ttctccttga tatttaaatt ttaatgatat agtatcaaat
     1681 ttttttatta tattaaatgt gtaaaatgac taaatgtatt tgtttaatat gtatatttaa
     1741 tttttagcaa agaataaaac taacagtaaa atatatttaa aaaattaaaa aacaaaatta
     1801 aatttattat caaaaaatca ttttatgata tgttttattt tttatattac aaatagagaa
     1861 taatgaatta tactagaaag taggaggtga aaatatgagc agaatttcaa tttttatgga
     1921 gtcatgtaat ggcaaatttc caaatgaaaa ttcaatgttg tcagtttcct ttataaaaaa
     1981 tattttaaat gcagaaaaca aagattttgc taaaaatata tttaactatg gggaagaaag
     2041 aaaaaacaat aagcaaataa aggatataaa tacagccatc tacataccaa atcttataag
     2101 aagcaaaaat gagttgcttg ttgatggaga tataatattc aatataagtt tttataatta
     2161 ttctatgttc agtaaattat acaatggaat tttaaaagta aaggagctag aatataaagg
     2221 ttatagattt aaaataaaaa atataaaaat acataaagaa cacgagataa aagaaaatgg
     2281 tgttatattt aaaactatgt ctcctataat agtaaagaat aaagaaggta aatatttaga
     2341 tgtagaagat tcaaattata tagaatgttt aaactatata gctaatctaa ctctggagag
     2401 tataagaggt agtgggctta gaaagccatt ggaatttata cctcttaact tcaaaaaaag
     2461 agtgttaaaa gaaaagattc gtggatttaa agtgagagaa tattactata taaatgctta
     2521 tgcaggaaca ttttttttaa aaggaaatat ggaagattta aatgcactgt ataaaatggg
     2581 aattggatat agaaggacgg aaaatgcagg aatggttgat atattaaagt agcgaggtga
     2641 gagaaataaa agctaaattt tatagatcag gtgatattgt aagagatatt ggcattgttt
     2701 acttttatga attactaatg gatttaaaaa acgaactaga aaaaaatgat tataagctaa
     2761 aatttacttg tgagttaaat agaaattatt tatctttaga atctgcagaa tatatagacc
     2821 ctaagtatat atctgattat atattagaaa atcaactttt taaggtattt aaaaatggga
     2881 aaaaggaaac cttagaaaaa atatttagta atatagataa tttaacgtat gtaaatttta
     2941 ttgaagaatt agataaatct tctgcaactg agaaacaaaa ggaagggatt aaaaaagatt
     3001 ttaaaaacag aagatttcca tatgttagaa atagtggtaa gtatggatta aacacgagtt
     3061 tagaaaatat ggaaacaaat gtaaaaagaa tagtagatac tgtaattaaa tcaaatatag
     3121 agatagatga aatagataaa cttgatttga cacaatatga agaaaaagat agtgtatgta
     3181 atgtttgtaa tataaataaa acaacaaaac tagatattga tttaagagag cgaaaggact
     3241 ctaagtataa ctttttattt agaggtgcag aaaaatcagg ctttaaaaga agtggacaag
     3301 ttgaaagtaa tatttgtttt gagtgtgaat ttttcaactt aatgtgtctt ttatatataa
     3361 atttaaaaag accaatggtt tttgcatata cgaatgattt aagggaatta gcatttttaa
     3421 atcataagat aatgcttaaa agaaagatgt attcagataa atcattttat aaaaaacttt
     3481 tgcatgaaaa aataagttcg attagactat ataggtttga tattgatacg aataaaggta
     3541 taattctaaa gtttgactct attatagagt ataaagaatt attaaaaaaa attgaactta
     3601 tagatattat agataattat aatttttcaa gagagccagg gaatactaga aatttgggta
     3661 aaaaaatgat tgataatggg aatttatcaa atttaaaaga attattactt gataatataa
     3721 gtgttttaag gcaactaact ggaacttctc gagaaatgga ttttgtatct agctcatata
     3781 atatcggttt atatataaaa ttgtgttgga ttgtagatgg aggggaggag tataaaatga
     3841 aaaactatat ggaaagtaat ttaatatatt caagagtagg taaagactta tttaataaaa
     3901 tgacagatga atccagaaga aaaaattttt ctatgaaagt gattcaacta cttaagtcta
     3961 atgatagaac tcagctattt caaactataa tgcatgtgtt agtttctaat ggaatcttaa
     4021 taggagaagg gtttgtagaa ggtctaatgc agagttctga attagaatta aatacgaatg
     4081 taggtctatt tattcaagaa atcatgaaat aaaaaagggg gaaaatttaa aatgaatgaa
     4141 aaaagagtaa aaggagtaac attaactata ctgacagaaa gcccagttcc actttcatat
     4201 gaccaagggt atggaaatta tacaccaata aaaaaagaac aatatagaga aaaaatacat
     4261 gcaaaaacta gtatagccac aataacatat gatttaagaa gaatgctcca ccaagaatat
     4321 ggttggaatt taagtaatat tatttttggg aaaaaaggaa atatatatcc atctattaag
     4381 caaaatgtag gatgtgtaga tgaaaatgga ttagagactg atgtatttgg ttatttgata
     4441 ccacttaaag ataaaggttc tataagtaag gcttctccgc ttagaataat accttttagg
     4501 tcattaaatt catatagagg ttcaacacag ttgataacaa atagaggttt tttaagtagc
     4561 gaatttggta gaaaatatta tgatgagaag gaagaaaatg aagtacttag agatgaaaac
     4621 tttccaacat cacaagcatt agcaatagaa gagactttat cagattacta cgtttataca
     4681 ataacattgg agcttgatag aataggagta gtagaagtag aagatggaaa gttgctttta
     4741 ccagaagaaa gaaaatttat gagtaaggag ttaagagaaa aagcagtaaa agatatatta
     4801 gatgcaatta ctgtgtttac aagaaacata aaacattcat cagtactgtt aaaaccactt
     4861 gcagtaatgg gtggagcttt tgataaagtt gtaccgtttt tttgggatga tgtagattat
     4921 aatgctgata gtggtgagat aaatttagag ggagtcatag aaactataga aagttatagc
     4981 cttaaagaaa gtaacacaat acttgctatc aatgacaaat taaaaatttc taataaaaag
     5041 gaattaaata agtttaattt aagtaaatac cctgtaaaag agattaaaaa tctagcagat
     5101 agattggaaa ttggagaaga taatatgtgg tatttaaaag agtaggaggt tttttgaatt
     5161 atgaaagtat tacaatgtaa acttaagcaa ccaactgctc actatagaga cccaaaagta
     5221 tttcaaaatg aatatataag tactttaaat ttaccatcta agaccacaat aatgggaatg
     5281 ataacgtatt tatgtgatag aagattaaac tctgatatag atataggaat aattggaaca
     5341 catcatcata gagaactaga attttcaaga ggagagaaca ttgatttttg gaatgaatat
     5401 acaaatatga gaaaaggcaa agataaagaa aagtttttgc ttcaaggaaa ctactatgat
     5461 tactataaag aacataaagc tcaaaatagc atattaaact atgaagttct taaagaagta
     5521 gaactgacta tatttatttc ttgcaaggat gatgaagaac ttgaatttat taaaaaaaag
     5581 cttgaaagtc catgtaaata tgcaaattta ggaagaaaag aagattttgt aataccaagt
     5641 gaaaaagggt gttttgtgaa agaagttgag ttaaaagagg ttatgcctat gaatactcgt
     5701 gatgctataa aagaaaatat aaaattaaag aatacatatg taagagttga cttaagagat
     5761 aaagagaatg tcgagactat aataaatcaa ggtgtattga ttgcattacc ttataaatat
     5821 aaagatttag aagcaaatag agatgataga gtttatgagt tttgccatta tatatatgtt
     5881 gataatgatg gtatatatcc taaaaatatt aaggtaaatg tatgtaatgt atgtattgat
     5941 acaaaagaag tttttacatg gctttaaatt attttgaaag ataggtgttt atcatgaaaa
     6001 atatcatgca aaaagaaata ttagctaagt catataattt aagaactaat caacaagaac
     6061 aaacattagt tgaacatatt aaggatttat ttgaagtatt agaatctata ttagaactaa
     6121 atctatattc agataaagat gtagagatac taaaaatatg ttgtgctttg cacgatttag
     6181 gaaaaattaa ttcaattatg caacaaaaaa tggagattaa taataaaata tcgtatagtt
     6241 gctcagaaga agagaggaaa aagttagagt ctgacaaaaa atctttaaaa aagat
//
LOCUS       DACTGW010000203         6271 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.203, whole genome shotgun sequence.
ACCESSION   DACTGW010000203 DACTGW010000000
VERSION     DACTGW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.203"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            99..980
                     /locus_tag="I9573_003039"
     CDS             99..980
                     /locus_tag="I9573_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HAT4797636.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSNKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1362..2165
                     /locus_tag="I9573_003040"
     CDS             1362..2165
                     /locus_tag="I9573_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797637.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            2177..3307
                     /locus_tag="I9573_003041"
     CDS             2177..3307
                     /locus_tag="I9573_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT4797638.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLRESIDEPIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEGFVKSYFESYSLIRG"
     gene            3311..4720
                     /locus_tag="I9573_003042"
     CDS             3311..4720
                     /locus_tag="I9573_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797639.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDMDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDVEIDYSP
                     ERTHDIWDESNEIDKNTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            4943..5440
                     /locus_tag="I9573_003043"
     CDS             4943..5440
                     /locus_tag="I9573_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HAT4797640.1"
                     /translation="MFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPIYSNN
                     TVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRGGKCE
                     ELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLSEEFATILDISPNEVSSYIS
                     SHIPQ"
BASE COUNT         2559 a          556 c         1106 g         2050 t
ORIGIN      
        1 aagattaaaa gacataatta aatacgtatg agaggtttta aatagtattg ttaatgaact
       61 tgattattgt ttataattac aaattggagg agataaatat gaaaattact gaaggatata
      121 tgccttttaa aggttttaaa acgtattacc gtatagtagg agaaaatacg gaagggaaga
      181 agccattagt attacttcat ggaggtccag gttctacaca caactatttt gaggtattgg
      241 ataagatagc tgaaagtggt agacaagtaa taatgtatga ccaaattggt tgtggaaatt
      301 cctttgtaga agggcatcct gagttattta atgcagatac ttggatagag gagcttattg
      361 aactaagaaa acatctagga cttgatgaaa ttcatctgtt aggtcaatct tggggaggta
      421 tgcaggctat ttggtatgct attgaatata aaccaaaagg tattaagtct tacatccttt
      481 catctactct ttcttctgca aaactttggg agaaagaaca aaaaagaaga atatcatata
      541 tgagtgaggt tgaccaaaaa gcattgcttg atgcagtaaa tactggagat tactctaata
      601 aagaatataa tgatgcatta gaaagattta tggaaatgta ttgtgcaggt gaagtgacag
      661 aagattctcc agagtgcctg agaagaccta aaaaatcagg ttctgaagca tatattgtag
      721 gatggggaca aaatgaattt tctcctacag gaactctttc tggatatgaa tttacagata
      781 gattacatga aataaaagag ccttgtctag taactagtgg agcaatagat ttatgttcgc
      841 catatattgc aaagactatg tatgatagaa tacctaatag taagtgggag ttatttgagt
      901 attcaagaca tatgccattc gttgaagaaa atgagaaata tataaaagtc ttaactgagt
      961 ggttgaatgc gaacgattaa aaactatata aatttatata aagaaatgag tgtctcaaaa
     1021 tgaacttttt agttcattag gcactctttt tgcatgaaat caaatatatt ttcattattt
     1081 gaacgataaa atgaggctta tatctatttt gggaaagtaa ttttttatta ctagcgttag
     1141 attctaatct aattgtatac ttattgaaat aatagaaata tattggtgtt tataacttaa
     1201 ttttattgtt ttttagaaat ttttttatat attggtataa ttattctgtt taaatttgat
     1261 taaaaagtat attaattata ataagaaaca attatataaa taggaataga ttattataat
     1321 taatatagat ataaaaataa ttatagaaag aaaggcacat tatggaaaaa ttgagagtta
     1381 ttgaaatgat aaaaaataat cccaatatta tagctacaat agataatcca actgatgaaa
     1441 tgaaattgtt agctattaaa gaaaatgggc ttgtactaga atatataaat aatccaacca
     1501 gagagatgca agaactagca atagataata atattagagc aattaaattt atagataatc
     1561 caacagaaga tatgatgata aaagcagtaa atgagggatg gagtatactg gattatatca
     1621 aaaacccaac tgataaagtt attgaaatgg ctataaatca agcaggatgg gcaattaaat
     1681 atgctaaaaa tccaagtgaa gaattacaac tgttagcagt aagaaaaaat tatgattcga
     1741 taaagtttat aaaacaacct tatgaaagtg tacaagagga ggcagtaaga ataagttatg
     1801 atgccttgag atatatagat tcacctagtt ataatgcaga acttatagct ataaaaaata
     1861 atgaagcagc tattagcttt ataactgatt tagataaaaa taaaatgttg gaatttttaa
     1921 aagtgaatat tttagtgatt aagtacattc tgaaaaaagt atctaaagat gaattagaga
     1981 gtgtattaaa agaagtatta tccaaagaag atgttgagga aaagtatatt agagattttt
     2041 tgaattgtag tgttatagat agaaatagcg aaagtatacc aatagataaa attatgttta
     2101 tttataaata tggaagtaag acagcaaaaa aaatagctgt agatgagaaa ttaaagatta
     2161 tgtaggagag agaaaaatga attttataga cacattaaaa agcgttgact taatattatc
     2221 cactggagaa gtacctttaa tagtaggtga aagtggaata ggaaaaacag ctttagcaaa
     2281 taagcttgcc aaagaaaatg attggagttt aattgttatt gatggaaact tacttaaaga
     2341 gggtgaaata ggtggtcttc caactataga atcttatgta ggagttaatt ctaatggata
     2401 taagactgaa aagaaaacaa cagtgtatgc tgttcataac aaattaagag aaattgatga
     2461 agagatatct aaaagtaaga cagttctttt atttatagat gagataaatc gttgtgagca
     2521 tacagtacag caagaactta tgaatttaat tttaaataga gaaattaatg gatataaact
     2581 gcatgatgat gtaaagattt tagcagcaat gaatccatca agtaaatatg gttcagattt
     2641 tgattatcaa gtggtagata tggatgcagc ccaagaaaat aggtttgttt ggttaaatat
     2701 ggagagtgac catacacaat ggataaaatg ggcaatagat gagggtattg aaagaaaagt
     2761 tatcgagttt atttccactt ttccagaata tctgcataaa ataaatgaag atgatgtaag
     2821 agctactcct agaagttatg agagagtatc taaaatttat aaagtttata aagaaaaaaa
     2881 taattctatt cctagagcag tatttttaaa tgttgtaaag ggtaatgtag gtaaagttat
     2941 tgcagaagaa tttataagtt ttattgaatc aaactctgaa ccattaatat cctatgaaga
     3001 tgttttttta agagaatcta ttgatgagcc cattgtagaa agagtaaaaa atgagagtca
     3061 tacaagactt tatctatcag ctatgaacat tctaaaagac ttagaattaa atattaagaa
     3121 tgataaatat gaatcaaacc actatattaa tagatttatt gaatttttaa aaatgtatcc
     3181 tgtagattta atgataggta ttatgaagga tattagaaac agttatattg aagtatataa
     3241 aaaagccata gaaaatgaag gttttgtaaa atcatatttt gaatcttata gcttaataag
     3301 gggataatgt atggaaaatt attttgataa acaagcaaaa gagctttata ataaagcaag
     3361 tgaaattata aatactcatt ctatgctaaa ggcaaatcgt gctaatgaga aatttgatat
     3421 ggatatacca caagatttta agtatgagtt ttttagtctt gtagataaag tgaatttaag
     3481 tcttatggaa gaagaagata atttttatgg atattttttg tttcagatgt caagagaaat
     3541 aagatttgat ataagtagtc ctacaggcgt gaattttaag ggagctaaat atgttatata
     3601 ttttaatcca ataatctttt taacgcttga cataaagcaa atggagacta caattaaaca
     3661 tgaaattcat catatattat ctatgcattt gatgagagca aaggagttaa aaggaaagta
     3721 tagtacattg gcaattaata tggcaatgga tatagtagta aataaatttc taaacaattt
     3781 accaccatat gcaactacct tagagtgggt aaatttaaaa tattccttaa aacttgaacc
     3841 atatgaacct tttgaatatt atgtagaaaa gattcagact gaattagatt tgatggaagt
     3901 ggatgaagat ggtgaggaag atgatagtga taaatatgga gatgtagaaa ttgattatag
     3961 ccccgaaaga actcatgata tatgggatga atccaatgag atagataaaa atacccttag
     4021 agaatttact gaaaaattta ttaacagttc tcaaaaaggt aaagttcctg cttatttaga
     4081 aagtatgata tcatcactta aaaatagtaa gggtgaattg ccatggaatt tatatcttaa
     4141 tagaataatg ggaacagttg aaaataataa aaagaagact ataacaagaa gaaatagaag
     4201 acaaccaaat cgtttagatt tgaggggaga acttagaagt cataaggcag aaatagcagt
     4261 tgcacttgat gtaagtggaa gtattagtga tgaagaattt aaacaagcta ttaaagaagt
     4321 tcttagtata gtgaaaaact ataatcatga aattacaatt atagaatgtg ataatgaaat
     4381 tagacgtgta tataaagtga gaactgaaaa agatataaaa gatagaatta ggataggtgg
     4441 aggtactaga ttttctccag tctttgagta tgctaataat aaaaagatta atttgttagt
     4501 ctactttact gatggtaaag gtgaaaataa actacaagta atacctagag ggtataaaat
     4561 tttatgggtt atttctggaa gaggagataa aatttcatta aaagaacctt atggagcagt
     4621 taaaaaactt actaaggtta aaacaaatga tgaaatatta gaaataaatg atgtaagaaa
     4681 tgatgggtac tcaatgaata gccaagcacc aattttatag gtattcacag gagataagct
     4741 gtattaattt attttgatat gcttgttata aaaatctaaa aaatacatta atcaatggtt
     4801 aatgtatttt ttaattttaa taatataagt tttacttaca tttaagtctt aaaatgtgtt
     4861 aaaatataat atatagcaga tagttttggc aagatggatt tagttgtcaa aactattttt
     4921 tttattaagg atggtgatgt ttttgtttaa gattgatgat tatataatgt atggtatgac
     4981 aggagtctgc aaagtcatgg atataacaaa tgaacgattt acaaatggaa taaaaaaaga
     5041 gtattatgta ttgagtccta tttattctaa taatacagta ataaaaattc ctgtagataa
     5101 tgaaaaagta ccaatgagaa agttactttc taaaattaat gtgctttcat taataaatga
     5161 tattcctaat atggatacat catggataga taatgaaaaa ttaagaagtg agcaatttaa
     5221 aaaaatactc agaggtggta aatgtgaaga gttaataaaa ttagtaagaa gtatagataa
     5281 taacagagag tatgtaaaat ctattggtaa aaaaacacat caagcagatg ataatataat
     5341 gaaagaagca gaaaggttat taagtgaaga atttgcaact attttagata tttctcctaa
     5401 tgaagttagt tcatatattt caagtcatat acctcaataa agtgttttag tatcctttat
     5461 ttaatctatt ttaagtatta tttatgtatg ctggttttgg taaaataaaa tatataatta
     5521 aattatataa aaaattaaga atgattgtat tgaagaatta cagaatagtg cggagagccc
     5581 actcctttag gtgtgagatg gatatcactt aaagatttaa attgttattg tagatgtttt
     5641 taatataaag caagattaat aagtgtattg taagctttaa gtgaaattga tactataatg
     5701 agttacttag attttaaaat atataatatt ataaaaaatc cttttaggtt ttgaatgaat
     5761 aagatggata ctatgttgaa tagaatccat cttatttaag ttttatttat gttgtttatt
     5821 tgtaagcatt atttttgtac atgaaaacac agtgtaaaaa tatgcttaga attatgaagt
     5881 tttattaaaa atatattcga caaaataaga ctaaaaatat tcttaaactt ataactaaga
     5941 taaatattgt atatttatag tgtagcctga aaaattgtgg atgtacgaaa tttatttagt
     6001 attttattga aacaaagtag aagaagtact atagtttatc tttgaataga ttgttatttt
     6061 ttagtgaaaa agttattagg ttaatacata aatataatag cttataaata gtttaagcat
     6121 gttaaaaaag tagttattag agatatatag ttacattgct tataagatac ttaaaatgat
     6181 agcattcact tatatttaga aaattaagat aaaaaatcat ttttatatag taaagcatac
     6241 ctaaatttag aaaataattg aatttataaa a
//
LOCUS       DACTGW010000204         6265 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.204, whole genome shotgun sequence.
ACCESSION   DACTGW010000204 DACTGW010000000
VERSION     DACTGW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.204"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..788
                     /locus_tag="I9573_003044"
     CDS             <1..788
                     /locus_tag="I9573_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HAT4797641.1"
                     /translation="PEESMVKVVNGKVMVLRADITTSIIKSIVPRWEDGLKLKLFYNS
                     SIYKNKNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYI
                     HGLLEELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYN
                     VIEKSNKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGY
                     YPNSYKEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            810..1436
                     /locus_tag="I9573_003045"
     CDS             810..1436
                     /locus_tag="I9573_003045"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAT4797642.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1470..2519
                     /gene="hisC"
                     /locus_tag="I9573_003046"
     CDS             1470..2519
                     /gene="hisC"
                     /locus_tag="I9573_003046"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAT4797643.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2522..3100
                     /gene="hisB"
                     /locus_tag="I9573_003047"
     CDS             2522..3100
                     /gene="hisB"
                     /locus_tag="I9573_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HAT4797644.1"
                     /translation="MRIWKVERNTFETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3102..3707
                     /gene="hisH"
                     /locus_tag="I9573_003048"
     CDS             3102..3707
                     /gene="hisH"
                     /locus_tag="I9573_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAT4797645.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDITLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELVAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            3704..4426
                     /gene="hisA"
                     /locus_tag="I9573_003049"
     CDS             3704..4426
                     /gene="hisA"
                     /locus_tag="I9573_003049"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAT4797646.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGINTKIIEDIANNIDIPIQVGGGVRDKGKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSDVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4408..5166
                     /gene="hisF"
                     /locus_tag="I9573_003050"
     CDS             4408..5166
                     /gene="hisF"
                     /locus_tag="I9573_003050"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAT4797647.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKNMGVEVRL"
     gene            5194..5862
                     /locus_tag="I9573_003051"
     CDS             5194..5862
                     /locus_tag="I9573_003051"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAT4797648.1"
                     /translation="MDNKCNNVYSDEVEEFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQKLWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMEPEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2566 a          521 c         1217 g         1961 t
ORIGIN      
        1 ttccagaaga atctatggta aaggttgtaa atggaaaagt aatggtactt agagctgata
       61 taactacaag tataataaaa agcattgtac caagatggga agatggttta aagcttaagc
      121 ttttttataa ttcatcaata tataaaaata aaaatacagt tggaataaaa gagataagac
      181 aaataggttg cgagtatcta ggagaagcct ctgtagaagc agatagagaa gtagtgaaat
      241 tagctcttaa gatacttgaa aaatacaata ataattttat tttagaggtt gggagtagca
      301 agtatattca tggtttgtta gaagaattaa atttaaataa gaattgtgaa aatcaaatta
      361 aaaatttact atatacgaag aacactcatg aattaaaagt atatattgaa gaactgaaaa
      421 ttaagcgtga agtaaaagag ctattatcaa atattttaag cttgcaaggg aatttataca
      481 acgttattga aaaatcaaac aagttctatt gtaataataa gatgaagcaa gctcttgaag
      541 aattaaagca agtaaataat ttaattgagg aatgtaattt tttagataag gctagatttg
      601 atttatcaat gattactatg cttgactatt atgaaggaat tatgtttaga ggatactacc
      661 caaactcata taaagaaatt cttagtggtg gaagatatga ttctttaact aaagagtatg
      721 gaaaagaaat tccagccatt ggatttacac taagtgttga tgagttaatg aagtatgtat
      781 ataagtaaag tttagaagga gggattgctt tggattattt gactatagca ctagcaaaag
      841 gacgaattga aggggaatca tttaaaaaat ttaaaaaaat gggtctagga gatagtatag
      901 acacagatac gagaaaacta atttttaaag atgaagaaaa taaaattata tatatacatg
      961 taaagccatc agatgtagtt acatatgttg aaaaaggagt cgctgacctt ggtatagcag
     1021 gtaaagatac tattttagag aatgagacag atgtatatga aatatatgat ttaggttttg
     1081 gaaaatgtaa atttgctgta gcaggattaa agggagatag catatataga gaagatgaat
     1141 atctaaaagt ggcaacaaag tatcccaata tagcaaaaaa gtattttaaa gagaaaggtc
     1201 aaaaaattga aataataaaa ttaaatggtt ctgtagaact agctccaata gtagggttgt
     1261 cagatgtaat tgtagatata gttgagactg gcaatacact taaagcaaat ggtcttgaaa
     1321 tattagaaga catatgtaat ataagtgcta gaattatatc aaatagagct agctatagat
     1381 ttaaatatga acaaattcag aatataattc gtttatttga agaacttgat aattaaaata
     1441 taattgaact ttataaggag ggatttaagt tgagagaaaa agaaagtata agagagttaa
     1501 ggggatatga accaaatcac gtaaactgta aagtaaaact tgatgcaaat gaaggaagta
     1561 agaggttgtt taaatacctt attaaagaaa tttcagatag tgatatagat ttaaatttat
     1621 atcctgaaga ttcttatagt gatttgaaag aatctataat agattacata aatatttctg
     1681 gagtaaataa gaaaaatctt ctagtaggaa acggttcaag tgaaataatt gatttaataa
     1741 tacatacttt tgtagataaa gatgaagtaa tattgagttt ctctccaagt tttagtatgt
     1801 attctatata tagtcagata aatgggtcta aatttatagg tgttgaaagt gatgaaaatt
     1861 tagttataaa tatagataat gtgattgaaa aagtaaagga gaataatcct aaaatagtaa
     1921 ttgtatgtaa tcctaataat ccaactggaa caatattaaa gagggaagag ataataaagc
     1981 ttttagattc aactaatagt ctagtggttt tagatgaagc atatatggat tttggagaag
     2041 aaagcatgtt aagtgatgtt tttaagtatg ataatctaat agttcttaga actttatcaa
     2101 aggcatttgg attggctgga ataagaacag gatatatgtt atctaatagt agtttaataa
     2161 attctgttga gaaagtaaga cctccatata atttaaattc attatcagac tttattgcta
     2221 caagagcact tagaaataaa gatgtggtaa aggcttatat aaaggaagta aaagaggaaa
     2281 gagaagtttt atatgaagaa atgattggta tgggaattaa agcatacaaa tcacaagcaa
     2341 attttatatt attttattct gaaatcgaaa atttatcaca aaaacttatt gatagaggag
     2401 ttttaataag aaaatttggt ggaaagttag aaaattatta cagagttaca attggtgata
     2461 aagaagaaaa ttctatgttt gttggagcta taagagatat tttgaaaaag gagaaatagg
     2521 tatgagaatt tggaaggtag aaagaaatac ttttgaaaca cagattttag tagaattaaa
     2581 tattgatggt tctggtaaag ctgaaataga tactggaata ggatttttag accatatgct
     2641 taccttaatg tcatttcatg gcaagttcga tttaaaagtg atttgtaagg gtgatacata
     2701 tgtagatgac caccatagtg tagaagatat aggtatagct ataggtgaag catttaaaaa
     2761 tgcactaggt gacaaaaaag gaatacgaag atattctaat atctatatac ctatggatga
     2821 atctctatca atggtagcaa tagatataag taatagacct tatcttgtat ttaatgctaa
     2881 atttgatacc caaatgattg gtagtatgag tactcaatgc tttaaagaat tttttagagc
     2941 ttttgtaaat gaatcgagag taacattaca tatcaatctc ttatatgggg aaaatgacca
     3001 tcataagata gaaagtattt ttaaagcatt tgcaagagct ttaaaagagg gtagtgaaat
     3061 agtttctaat gagatagcat cttcaaaggg ggttttgtag aatgaatata atagtagact
     3121 atggtcttgg aaatatagat tctgtgtcta ggggttttag aaaagctgga atagaaacta
     3181 aaatttctag tgatatagat gaaataaaac aggcagattc acttatactt ccaggtgttg
     3241 gagcttttag agattctata agtgccttag ataaactagg attgatacca ataataaagg
     3301 agcatgtttc taagggtaaa tttatgatag gtatatgtct aggaatgcaa ttactttatg
     3361 aaaaaagtta tgagtatgga gaatatgaag gattaggact tataaaagga agtattgata
     3421 aactggatat aactttaaaa gtgccacata tgggatggaa taatctaaaa tttaataagg
     3481 caaatgatga tatacttaaa tatataaacg aagatgatta tgtatacttc gttcactcat
     3541 attatgcaaa ttcatcaaat gaagaactag ttgcattttc agaatatgaa aagaaaatcc
     3601 ctgctattgt tagaaaaggc aatgtatatg gtatacaatt tcatcctgaa aaaagtggag
     3661 aagtagggtt aaatatatta agagcatatg gggagatgat aaaatgataa tatttccagc
     3721 aatagatata aaagataata agtgtgtaag actcacacaa ggagaatttg acaaagtaaa
     3781 tgtatattat gataatccat tagaagtagc atataaatgg aaaaatgaag gagcagaata
     3841 tatacatata gtagatttaa atggtgctag aagtgaattt ggtataaata caaaaataat
     3901 tgaggatata gcaaacaata tagatatacc tattcaagtt ggtggtggag ttagagataa
     3961 gggaaaagtc aaaagtttaa taaatgctgg tgttactaga gtaatattag gtagtatagc
     4021 gattgaaaat ttgaatttgg ttgaagaatt ggtcaatgaa tataaagaga aaattgtagt
     4081 ttctatagac gctaaagatg gaaaagtagc agttagggga tgggaagttg taagcgatgt
     4141 agactcatta acattatgta aacaacttga aaagataggt gtacaaacta ttgtttatac
     4201 agatatatca aaagatggga tgttgcaagg gccaaatttt gacatatatg aaagaatagc
     4261 aaaggaaacg tcattaaatg ttattgcttc tggaggcgta acttctatag aagacgtaaa
     4321 aagactaaaa gctatgaact tatatggtgc aataataggt aaagcgcttt atgataaaaa
     4381 gatagacttt aaggaggcac aacaattatg cttactagga gaataatacc ttgtctagat
     4441 gtgagaaatg gaagagttgt aaagggaaaa aaatttaaag atatagtaga tgttgatagt
     4501 ccagaagtac ttggaaaatt ttatagtgat tgtggtgcag atgaacttgt attctatgat
     4561 ataactgcat caaatgaaga gagaaagaca tctttagaat ttgtgacaaa agttgcagaa
     4621 aatataaata taccattttg tgttggtgga ggagtaaata aattagaaga tttcactgat
     4681 attttaagaa aaggtgctga taaggtttct ataaattcat cagcagtcaa aaatcctgaa
     4741 ttaataaggg aagcttcatt aaaatttgga gcacaatgtg ttgttttatc gatagatgca
     4801 aagaaaaatg aagagggttc atggagcgta tacgtaaaag gtggaagaga aaaaactaat
     4861 ctggatgcaa ttgaatgggc tgttaaaggt gtagaacttg gagcaggaga aattgttgta
     4921 aatagtatgg acgaagatgg aatgaaaaat ggatatgata tagaattatt atcaaagata
     4981 acttctttag taaatgtacc tgtcatagca tctggaggcg ctggaaaaaa ggaagatttt
     5041 tatgaggcag ttaataaatc taatgtagat ggaattttag cagcatctgt ttttcatttt
     5101 ggcgagataa aaataaacga tttaaagaaa tatttaaaaa acatgggtgt agaagtaaga
     5161 ttataagtta aatactaaaa ggagattaga aatatggata ataagtgtaa caatgtatac
     5221 agtgatgaag tagaggaatt tataagaagt ataaagtttg atgataaagg gttagtccct
     5281 gttgttgttc aagaggtagt tagtaaagat gtactgatgt tagcatatat gaataaagat
     5341 gctataaaga aaaccttaaa agataaagta gcttgctatt ttagtagaag tagacaaaaa
     5401 ctttgggtta aaggtgagac atctggtaat actcaaaaag tagttaagat gtcttatgat
     5461 tgtgatgtag atactatact actttttgtt gaacaaactg gtgtagcttg tcatacaggc
     5521 aattattctt gtttttacag agacttgttt gatgatagtt caaaaatgga gcctgaagta
     5581 caaacaaata tattgaaaga attatatgat ttaatcaatg aaagaaaaaa taatccagtt
     5641 gaaggttctt atactaatta tctttttgaa aaaggaatag ataagattct taaaaaagta
     5701 ggtgaagaga gtagtgaggt aattatagca tcaaaaaata ccgataaaag tgaacttatt
     5761 tacgaaatta gcgatttagt gtatcataca ttggttttaa tgatagaaaa aggtgttgaa
     5821 attgatgaaa taaaaaaaga attacttaaa agaagaaaat agaataggat agattaatgg
     5881 taataagaaa atataccgct tttaataaaa agtggtatat tttttatttt atattttcga
     5941 tttatagaat aatatttaaa ttacttgata aaattaacta tatatataat ttattgataa
     6001 aataattgta gtacattatt tattttagaa aaaagataat tatctaatta tatttaagga
     6061 taataatatg aaaataattt ttatattaaa aaatatgaat gaaaatagta ggttttataa
     6121 aaaacatatt tagattttaa catctaaaag gaattttcaa acctaatatt tcatttttaa
     6181 ataaaaaaat aaaatattag ttttgattta tggtttaaat tgggttataa taaattgtca
     6241 aattatttat taatataaaa tagac
//
LOCUS       DACTGW010000205         6255 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.205, whole genome shotgun sequence.
ACCESSION   DACTGW010000205 DACTGW010000000
VERSION     DACTGW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.205"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            186..1208
                     /locus_tag="I9573_003052"
     CDS             186..1208
                     /locus_tag="I9573_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT4797649.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            1268..2275
                     /gene="gap"
                     /locus_tag="I9573_003053"
     CDS             1268..2275
                     /gene="gap"
                     /locus_tag="I9573_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAT4797650.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            2497..3699
                     /locus_tag="I9573_003054"
     CDS             2497..3699
                     /locus_tag="I9573_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAT4797651.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKANNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            3725..4468
                     /locus_tag="I9573_003055"
     CDS             3725..4468
                     /locus_tag="I9573_003055"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007791163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAT4797652.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLSK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            4480..6009
                     /locus_tag="I9573_003056"
     CDS             4480..6009
                     /locus_tag="I9573_003056"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAT4797653.1"
                     /translation="MKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTLI
                     NASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFANKVLCEAMDN
                     AKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALEY
                     AKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAIQ
                     NSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDGF
                     KREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAHV
                     TFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVLN
                     FANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPET
                     GKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLIS
                     K"
BASE COUNT         2501 a          728 c         1257 g         1769 t
ORIGIN      
        1 aaaaagaaaa aatactgtga tttgaaaatg gtggagaaat attttataaa ataaaaaaat
       61 aaaaaagttt ttgaaaaact attgaaatct atacgcaaat cctataaaat aaaaatatct
      121 tgtgggactt aaataaacac tagggacaaa aaaagtcccg aaaggaaagc aataaggagt
      181 atgttatgaa gaacttatta gaaattcaaa agaaattgat tccacaggca attgaactta
      241 tggaaagaag atactctatt ttaagacaaa tatctttaag tgaaccaata ggtaggagaa
      301 cattatcaaa tgtattagag attagtgaaa gaataatcag gtcagaaact gagtttttaa
      361 aagaacaagg acttatagac gtagctgtat ctgggatgac tatcactaaa gaaggttctg
      421 atttgttaga taaacttaag gacatcatga gtgacatcat gggattgtct aagttacaag
      481 atagagtcag agaaaagctt ggaataaaaa gggtaatact agttcctgga agctgtgatg
      541 aaaatggaag tttactaaaa gatgtaacta gatatggatg cgagtatttc ttaggcattt
      601 taaaagatgg agatatcgtt tcaataacag gaggaagcac tatgttggag ttttcaaacg
      661 ttattaaaac tgataagaga tatccaaact ctacaatagt tccagctaga ggaagtatgg
      721 gaactgatgt agagatgcaa tctaacaata tagttgcttc aataagtaag aaattacatt
      781 ctaattgcaa acttttacat ataccagatg agttggatga aagtgcaatg aaaactctta
      841 gccaagtgcc agagattaag acaactttgg agtatattaa aagaacagac attttagttt
      901 ttagtatagg tagagcagat gtcatggcaa agagaagaaa acttccagaa gacaaggttg
      961 atgagatatt atctaaaaat gcagttggag aagcatttgg atattatttc aacaaagatg
     1021 gagaaatagt ttacaaactg aacactgttg gaattgattt agaaacagtt aaacaagtaa
     1081 aagaaactat tgcaatcttt gcagggactg aaaaagtaga agccttatta gctatatcaa
     1141 atatgcataa aaacatggtt cttgtaacag atgaagaaag tgcttataag atattgtcat
     1201 tattataaaa gtcaagacct agcacagagc tagaaaacat acatttactt tataggaggt
     1261 agcgaaaatg gttaaagtag ctataaatgg atttggaaga ataggaagat tagcattaag
     1321 aaaaatgatg gagcaacaag ataagttcga agttgttgca ataaacgact taacagacgc
     1381 taaaatgtta gctcatttat ttaaatatga tacagctcaa ggaagattta acggagaaat
     1441 agaagttaaa gaaggtgctt ttgtagtaaa cggaaaagaa ataaaagtaa cagcagaaag
     1501 aaatcctgct gacctaccat gggctgaatt aggagtagat atagtattag aatgtacagg
     1561 attctttaca tctaaagata aagctgaagc tcatatacaa gcaggggcta agaaagttgt
     1621 tatatctgca ccagctactg gagatttaaa aacaatagta ttcaacacaa acagtgacat
     1681 attagatggt tctgaaacag ttatatcagg agcttcttgt actactaact gtttagctcc
     1741 aatggctaaa gtattaaatg ataaatacgg tatagaaaaa ggattaatga ctactataca
     1801 tgcatacact aatgaccaaa atactttaga tggtcctcat ccaaaaggtg atttaagaag
     1861 agctagagct gctgctggaa acatagttcc aaacactaca ggagctgcaa aagcaatagg
     1921 cttagtaata ccttcattaa aaggaaaatt agatggggca gcacaaagag taccagttgt
     1981 aactggttca ataactgaat tagtttgtac attaggaaaa aatgttacag ttgaagaaat
     2041 aaatgcagct atgaaagaag cttcaaacga atctttcgga tatactgaag aaatgttagt
     2101 atcttctgac ataatcggaa taagctacgg ttcattattt gacgctactc aaacaaaagt
     2161 tatggaagta gatggaaaac aattagttaa agttgtttct tggtatgata atgaaatgtc
     2221 ttatacttct caattaataa gaactttagg atactttgca caattagcaa aatagtcagt
     2281 tttgagaaaa tagaaaagta gattgacaaa gtccgagttt gtagtttaat actacggact
     2341 caggactttt tttaagaaaa aatatataaa atacacctaa ataatgttac atactatata
     2401 taaaaatatg ataatatgat atatttaata gggtaaaagt atgtactata ttatacttgg
     2461 caataaaaaa ttactttatt aaaaggagag atatttatgt caatgcttaa caaaaaaact
     2521 atagaagata ttgatgtgtg cggaaaaaaa gtattagtaa gatgtgactt taatgtgcca
     2581 ttacaagatg gtgtaattac tgatgaaaat agattgaacg gagctttacc aactattcaa
     2641 tatttaattt ctaaaggggc aaaagttata ttatgctctc acttaggaaa gccaaaagga
     2701 gaagctaaac cagaattatc tttagcacca gtagcaaaga gattatctga gatgttagga
     2761 aaagaagttg tatttgcagc agacgataat gttgtaggag aaaatgcaaa gaaagcaact
     2821 gaaaaaatgg aaaacggaga tgtagtatta ttagaaaata ctagatatag aaaagaagaa
     2881 actaaaaacg aagagaattt ctctaaagaa ctagcatcac ttgctgaaat atttgtaaat
     2941 gatgcatttg gaactgctca tagagcacat tgctcaacag ttggtgcagg cgagttttta
     3001 caagagagag tttgtggata cttaattcaa aaagaattaa aattcttagg agaagctgtt
     3061 gcaaaccctg taagaccatt tacagctata cttggaggag ctaaagtttc tgataaatta
     3121 gctgttataa acgaattatt agaaaaagtt gataatctaa taataggtgg aggaatggct
     3181 tatacattct taaaagctca gggttatgaa gtaggaactt ctttacttga aatagataaa
     3241 gttgaatatg ctaaagaaat gatggaaaaa gctaataata agggtgttaa cttattatta
     3301 ccagtagacg ttgttatggc agaccacttt gcaccagatg ctactccaat agtaacagaa
     3361 gatgctaatg taaaagaaga ttacatggga ctagatatgg gacctaagac tatagcaaac
     3421 tttgtaaaaa ctataaaaga atcaaaaact gtagtatgga atgggccaat gggagtattt
     3481 gaatttgaaa actttgcaaa tggtacatta tcagtagcta gagctatggc agagttgact
     3541 gatgcaacta ctgtaattgg cggtggagat agtgcagcag cagttaatca attaggattt
     3601 ggtgacaaga tgactcacgt atcaacaggt ggaggagcat cactagaatt tttagaaggt
     3661 aaagaattac caggtattgc agcattagat aataaataaa ctaatatata gaggagattg
     3721 taaaatgaga aaacctataa ttgcaggaaa ctggaaaatg cataaaacaa taaaagaagc
     3781 tttagaattt gtaaatgaag ttaaagataa agtaaattct gataaagtag aagctgttat
     3841 atgtgcacca tttactttat taaaggattt aaaagaagct actaagggta caaatataaa
     3901 gataggtgct caaaatatgc actttgaaga aaaaggagca tttacaggag aagtttcacc
     3961 tcttatgtta aaagaaatag atatggacta tgttgtaata ggacattcag aaagaagaca
     4021 atacttcaat gaaactgatg aaactgtaaa caagaaagtt ttaaaagcat tagaagtagg
     4081 aatagaccca atattatgtg ttggagaaac tttagaacaa agagaagctg gaaaaactaa
     4141 agatgtttgt aaagttcaag ttgaaaaagc tttagaaaat gttcttaaag atgatttatc
     4201 aaaagtagtt gttgcatatg agccaatctg ggctattgga actggtaaaa ctgcaactgc
     4261 tgaagatgct aatgacgtta tatcttatat aagagaagtt ataaaaggat tatatggaga
     4321 attagctaat gaagttagaa tacaatacgg tggaagtgtt aaaccttcaa atgtagccga
     4381 aataatgggt caaagtgata tagatggagc tttagtaggt ggagctagtt tagctagtaa
     4441 tgattaccta gaccttgtta atttctaagg agtgaaatga tgaaaaaacc agttgcttta
     4501 attattatgg acgggtttgg atacaataag gatgttaagg gaaatgcaat tgctgagtcc
     4561 aaaactccta atttagatag aataaaaaaa gagtatccaa acacattgat aaatgctagt
     4621 ggtcttgatg ttggtctacc tgatggtcaa atgggaaatt cagaagttgg acacactaac
     4681 ataggtgcag gtagaatagt atatcaagat ttaactagaa taacaaaatc tataaaagat
     4741 ggtgactttt ttgctaataa agttttatgt gaagcaatgg ataatgcaaa agaaaattct
     4801 cttcatgtaa tgggactttt gtctgatgga ggagtacatt cacatataga ccatcttaag
     4861 gctataatca aaatggcaaa agataaaggc gtacaaaagg tgtatgttca cgcatttact
     4921 gatggtagag atactgaccc tcaaagtgca ttagaatatg ccaaagaagt acaagctagt
     4981 atggatgaaa ttggagtagg tgaatttgct acagtttctg gtagatacta cgctatggat
     5041 agagataaga gatgggaaag agtagaactt gcatacaatg ccatggttag aggtattggt
     5101 gaaaaagcaa attctattga ggaagctatt caaaactctt atgatgatgg aaaaaatgat
     5161 gagtttataa tgccaacagt tattatgaaa gatgataaac cagttggaag tataaaggaa
     5221 aatgattcaa ttatattctt caactttaga ccagatagag ctagacaaat aactagagct
     5281 ttagtttgtg aagagtttga tggatttaaa agagaagata ttaaaaactt ctttgtatgc
     5341 ttaacagagt acgatataac tatagagaat gtgcatatag catttggacc tcaatcttta
     5401 gccaacactt taggtgagta tctagctaaa aatggaaaaa ctcaacttag agctgctgaa
     5461 actgaaaagt atgcacatgt aactttcttc ttcaatggtg gagtagaaga gccaaataaa
     5521 ggtgaagaaa gactattgat accttctcca aaggttgcaa cttatgattt aaaaccagaa
     5581 atgtcagcat atgaattgac agataaagct ttagataaat taggagaaga taagtttgac
     5641 tttatagtat taaactttgc aaatcctgat atggtaggtc acacaggaag tatagaagcg
     5701 gctataaagg ctgttgaaac tgtagatact tgtgtaggaa aattgataga taaaatagta
     5761 gagttaggtg gaagtgcaat aataactgct gaccacggaa atgctgaata tatgttagac
     5821 ccggaaacag gaaaaactgt aacggctcac tctattaatc cagtaccatt tattgtagtt
     5881 ggtcaagaat atgaaagtgc aaaattattg gatggaggaa gattatctga tatagcaccg
     5941 actgttttag atatgatgaa gttagaaaag ccagaagaaa tgactggtca ttcacttatt
     6001 tcaaaatagt cgattctgat aagtagagaa taatatataa actacaatat aaatttaaaa
     6061 ataatgcagt ttaggtttat tttgtaaagt ttagacttta tgacataaga tttagatttt
     6121 gtaagttctg gatattgtaa ggtgtagatg aaggtatatt ttataaaatt tacaagatag
     6181 acaatttata aaataaaaaa ttataatata gataataatg taaataatga tataaataat
     6241 aacaagatag acaat
//
LOCUS       DACTGW010000206         6183 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.206, whole genome shotgun sequence.
ACCESSION   DACTGW010000206 DACTGW010000000
VERSION     DACTGW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.206"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..973
                     /locus_tag="I9573_003057"
     CDS             <1..973
                     /locus_tag="I9573_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HAT4797654.1"
                     /translation="LINYLDKSICMGALSSGSTSVPKVLYRTYESWAGFFPIQNGIFN
                     ISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVISSGLNCKSWIRTIKQYNVTNIYLI
                     PTKLQLLVKHLKEPVLKVVSIFTGSQLLFEYTARNLKKYMPKSEIILYYGASELNYIT
                     YLCYDELIEKPLSVGRPFPGIDIYIRGGKIFVNTEYAVYNATKPYSVNDIGYYDNDGY
                     LIFEGRSDDIVNIGGFKVSSAKIENEVKKIPQIEDAIVLPYSDTIRGNQIVLFVITID
                     KITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTSSEKIDRLALLKML"
     gene            1049..1924
                     /gene="dpsA"
                     /locus_tag="I9573_003058"
     CDS             1049..1924
                     /gene="dpsA"
                     /locus_tag="I9573_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HAT4797655.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEVGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            1927..2517
                     /locus_tag="I9573_003059"
     CDS             1927..2517
                     /locus_tag="I9573_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HAT4797656.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(2725..2925)
                     /locus_tag="I9573_003060"
     CDS             complement(2725..2925)
                     /locus_tag="I9573_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797657.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            3429..6083
                     /gene="adhE"
                     /locus_tag="I9573_003061"
                     /gene_synonym="adhC"
     CDS             3429..6083
                     /gene="adhE"
                     /locus_tag="I9573_003061"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAT4797658.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYTQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDESADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDNAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRSPGKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEEPKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT         2354 a          762 c         1048 g         2019 t
ORIGIN      
        1 ccttataaat tatttagata aaagtatttg tatgggagct ttatcttcag gttctacaag
       61 tgttccaaag gtattatata gaacatatga gagttgggct ggattttttc ctatacaaaa
      121 tggtattttt aatatctctg gggaatcaat cttgtttgtt aatggaacac ttagttttac
      181 agggaatcta aattctataa tgtcagtatt atatgaagga ggtagtattg taataagttc
      241 aggtcttaat tgtaaatcat ggataaggac aataaaacaa tataatgtaa ctaacatata
      301 ccttatacct acaaaacttc aacttttggt taaacactta aaagaaccag tactcaaagt
      361 agtaagtatt tttacagggt ctcaattatt atttgaatat actgcaagaa atctaaaaaa
      421 atatatgcca aagtctgaaa ttattttata ctatggggca agtgaattaa attatattac
      481 atatttatgc tatgatgaac taattgaaaa acctctaagt gtaggcagac catttccagg
      541 aatagatata tatattagag gaggaaaaat ttttgtgaat acagaatatg cagtatataa
      601 tgcaaccaaa ccttattctg tcaatgatat tggatattat gataatgatg gatacttaat
      661 atttgaagga agaagtgatg acattgtaaa tattggagga tttaaagtta gttctgctaa
      721 gattgaaaat gaagttaaaa aaattcctca aattgaagat gcaattgtat tgccgtactc
      781 agacactata agaggaaatc aaattgtatt atttgtaata actatagaca aaattacgaa
      841 gaaagatttg ttgattaaaa tgaaagataa tctaatgaaa aatgaaatcc caaagaaaat
      901 tatcttttta aattcatttc catatacctc ttctgaaaag atagatagat tagcattatt
      961 aaaaatgtta taagtataat atttagtaat tttagtgaac caatgtattg ataaaaacat
     1021 attgtatata gtaaaggagg cttacaatat gtcaaatcaa tacgatataa ctgttattgg
     1081 gggagacctg cgacaagtgt acatggtaaa aaaactaatt aataaagggt attctgtagt
     1141 cacttatggg atatcaaatg aaattataaa tggaatcgtt ggaaaatcaa cttcactagc
     1201 tgaagcacta tccaaaagtt ccattatttt atgtccaatt ccatttacaa agaatcaagt
     1261 taatattgaa aatacggatg attgtcttga tgctactatt gataacttgc taattaattt
     1321 atctcctaat caaatattat ttggaggtaa cataccaact aaagtttgta aattttgcga
     1381 gcaacactct attcagattt atgattttat gaaaatgaat gatgttgcta ttttaaatgc
     1441 agttgctaca gctgaagggg ctatagcaga agctattaaa agaagtataa tcaatataca
     1501 tcaaagtaac tgtttagtat taggttatgg aagatgtgca caaatacttg caaaaaaatt
     1561 atacgctttg gatgcaaatg tatgcgttgg tgcaagaaat aatgatgcaa gaagtacggc
     1621 taatgcattt ggatattcca gtatttcttt aagtcaactt gatgaaaaac ttttacaatt
     1681 tgattatatt tttaacacca taccagagtg tattttaacg aaagaaagat tagaaaaagt
     1741 atcacaagaa gtaactatta tagatatagc ttcttctcct ggaggagtag attattctgt
     1801 tgttaaagaa gtagggatta atgcgactct ttgccttggg cttcctggta aatattcacc
     1861 aaagacatct ggtgagattc ttgttaacgc tatagaaaat attataaatg aaagaaggga
     1921 ttagaaatga gacttgaagg acttacaatt ggagtaggat ttactggttc gttttgtact
     1981 tatgataaaa tatttataga gttagagaat ttggttaaag aaggagctaa tgtacacact
     2041 atattttcag atgtatcaca aaatatcgat tgtagatttg gaaattctga agagtttatg
     2101 aaaaaagcat atgaacttac aggaaataaa cctatagtta cgattgaaga ggcagaacca
     2161 tttggaccta aagggattgc tgatattata ataatagctc cttgtactgg aaatactgct
     2221 gcaaaattag caaatggtat tacagattca ccagtattaa tggcagcaaa aggtcatctt
     2281 cgtaatgata aaccactagt aatatcaata tcaacgaatg atgctctaag ctttaatttc
     2341 aaaaatattg gtattctcct taattctaaa aatatatatt ttgtaccttt tggtcaagat
     2401 aattgcaaag caaagcctaa ttctatgatt gcacatactg agctaataat tccaactata
     2461 gaacttgctc tagagaacaa acagctacag cctgttataa aaagtccaca tcaatagaat
     2521 tggttcttat aatattatgt attagataca atactagcaa agaggctgtc tcaaaataga
     2581 gagaagcctc tttttcattg ttgaaatgat gaaaatatat ttaatttcat acaaaaaaat
     2641 gcatcatgaa ctaaaaagtc tattttgagc taatctccgt aatttttggt atattaaaat
     2701 tatttactta atccaatttt taaattagaa tttatatcgt tttgactctt caaatatcaa
     2761 tctaactgtt gttgatacaa aagaagagga cataattaaa acaaatactg atagtcctct
     2821 aataaacaaa ttatctacat aataagttac tattccaata cctccaatcg ttaaaattac
     2881 acttataatc aaagaaaaaa tgctaattgt ttttaatttt ttcatgctta tcctccttct
     2941 tataaacttc aaatttatct atataatatt aaatatattt tatttcatag tatataaaaa
     3001 tcatgaatat atattttttt aattatacta tatgttgtaa atgtttgtac atttaactat
     3061 atttacttta acttgttaat caagttatta ttttcatttt caagtatttt tatttttaaa
     3121 cttgatagaa tatcatatta aaaattcttt ttataaagtt ttatttgcaa aaaatacaaa
     3181 aatataaacg ttaaaaaaat aacgttatcc ttaagttttt agatgatttt attagtgatt
     3241 tgcgtaaaaa ataaagtgat atctagttaa attaagtatt tgtaaagcta gatattatta
     3301 gtagaaatga tgttatatta gataaaaaat aaaatgttaa aaaaataact tgaaaataga
     3361 aaaagtaaat gctataatgt taataaatta acataataaa taaaaaacta tctcaggagg
     3421 ttgttaaaat ggctcataaa gaacaagtaa taatagaaaa taaaaaagaa aatgaagtta
     3481 tgtcagttgg tttagatact gtaaataacg ttgaaagctt agttaaaaaa ctagcaaaaa
     3541 tcagagaagc tcaaaaaata tttgctacat atactcaaga gcaggtagat aaaatttttt
     3601 tagcagcatc tttggcagct aataagcaaa gagttcctct agctataatg gcacaaaagg
     3661 aaactggtat gggaattgct gaagataagg ttataaagaa tcattatgcc tcagagtata
     3721 tatataatgc atataaagaa actaaaactt gtggtgttat agagaaagac gaagcatttg
     3781 gtatgactaa aattgctgag ccaataggtg ttattgcagc agttgtacct acaactaatc
     3841 caacttctac tgctatcttt aaagcacttt tagctttaaa aactagaaat ggaataattt
     3901 tctctccgca tccaagagca aaaaattcta ctatagaggc tgcaaaagtt gttcttgagg
     3961 ctgctgtttt agctggagct cctgaaggca taataggatg gatagatgag ccatctttag
     4021 aattaactac aactgttatg aaagaagtgg atttgaccct tgctacagga ggtcctggaa
     4081 tggttaaatc agcatattca tctggaaaac cagctattgg agttggtgca ggtaatactc
     4141 cagcaataat tgatgagagt gcagatataa aaactgctgt aaactcaatt cttgtatcta
     4201 agacttttga caatggtatg atatgtgctt ctgaacaatc ggttatagta ttagaaaata
     4261 tatacaatga agtcaaaaaa gaatttaaag agcgtggtgc ttatttatta gataaagatg
     4321 aaacagaaaa aataagaaat ataattttag taaatggagg attaaattct aaaatagttg
     4381 ggcaaacagc ttgtactata gcaaaacttg caggctttga agtcccagta gatactaagg
     4441 ttttaatagg tgaagtagaa tcagttgaaa tagaagaagc ttttgctcat gagaaacttt
     4501 caccagtact tgcaatgtac aaggccagtg attttgataa tgcagttaga aaagcagaaa
     4561 aattagtaga agatggtgga tttgggcata catcttcatt atatattgat gatgtaaatc
     4621 aaagagaaaa acttgataag tttacttcag ccatgaaaac ttgtagaatt ttaattaata
     4681 ctccttcttc tcaaggtggt ataggggact tatataattt taaactagct ccttctctta
     4741 ctttaggatg tgggtcttgg ggtggaaatt cagtatctga aaatgtagga gttaagcatt
     4801 tacttaatat taaaacagta gctgagagga gagaaaatat gctttggttt agatcaccag
     4861 gaaaagttta tttcaaaaaa ggttgtttag gggtagcatt aaaagaactt aaagatgtaa
     4921 tgaataagaa gagagctttt attgtaacag atacattcct ttacaataat ggatatacta
     4981 aagcagttac tgatttatta gatgaaatga atattaagca cactacattc tttgaagtag
     5041 aaccagaccc aactttggaa tgtgctaaga taggcgctaa agctatgaga gaatttaatc
     5101 cagatgtaat aatatctata ggtggaggaa gtgcaatgga tgcaggaaag ataatgtgga
     5161 cattgtatga gcatccagat gtagatttcc aagacctagc tatgagattt atggatataa
     5221 gaaaaagggt atatacattc cctaaaatgg gtgaaaaagc taattttgta gctataccaa
     5281 catcagcagg tactggttca gaagtaacac catttgctgt tataactgac caagatacag
     5341 gggtaaaata tccattagca gattatgaac tgatgccaaa tatggctata gttgattctg
     5401 atatgatgat gaatatgcca aaaagcttaa catctgcttc tggtatagat gcattaactc
     5461 atgcattaga agcatatgta tctatgcttg caactgatta tacaaatgga ttagctttac
     5521 aagccattaa gagtatattt gaatatcttc cacgtgcata tgataatggt gcaaaagacc
     5581 cagaagctag agaaaaaatg gcaaatgctt ctacaatggc aggtatggct tttgcaaatg
     5641 catttttggg tgtttgtcac tctatggctc ataaattagg tgctttccac catgtaccac
     5701 atggtgttgc aaatgctctt ttgataacag aagttatgaa atttaattct tcagatgcac
     5761 caaagaaaat gggagcattc tctcaatata aatatccaga agcattaaaa agatatgctg
     5821 aaatagcttc atttttagga ttaaagggaa attctgatga agagaaattc caaagtttat
     5881 tggtagctat tgaagattta aaacttaaag ttggtattcc taaatctata aaagagtttg
     5941 gagttgaaga acctaagttt atggattcaa tagatgaaat ggtgattcaa gcatttgatg
     6001 accaatgtac aggagcaaat ccaagatatc cacttatgaa tgaaataaaa gatatgtatt
     6061 taaattcata ttatggaaga taaatttact ataaaataga taaaataaaa actcttatac
     6121 ataaaacaaa gttttattgt ataagagttt ttttgtttag aaaaattgat agtttttatt
     6181 ctt
//
LOCUS       DACTGW010000207         6145 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.207, whole genome shotgun sequence.
ACCESSION   DACTGW010000207 DACTGW010000000
VERSION     DACTGW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.207"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            93..803
                     /gene="rgaR"
                     /locus_tag="I9573_003062"
     CDS             93..803
                     /gene="rgaR"
                     /locus_tag="I9573_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HAT4797659.1"
                     /translation="MFRVVICDDEKIQRSILKEFTKKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
     gene            1867..2880
                     /locus_tag="I9573_003063"
     CDS             1867..2880
                     /locus_tag="I9573_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAT4797660.1"
                     /translation="MNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYIFISDKF
                     DDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNIRLINLS
                     LSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFTLDGDDS
                     FWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEKPGITIS
                     DLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCLDIEDNN
                     EKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLTELVERY
                     KNL"
     gene            3013..4311
                     /locus_tag="I9573_003064"
     CDS             3013..4311
                     /locus_tag="I9573_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAT4797661.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(4425..4670)
                     /locus_tag="I9573_003065"
     CDS             complement(4425..4670)
                     /locus_tag="I9573_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HAT4797662.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            4818..5684
                     /locus_tag="I9573_003066"
     CDS             4818..5684
                     /locus_tag="I9573_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HAT4797663.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(5688..>6145)
                     /locus_tag="I9573_003067"
     CDS             complement(5688..>6145)
                     /locus_tag="I9573_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HAT4797664.1"
                     /translation="TNSQITQVVLETEMMNYFSLQQLLSQLMESKFLTTYKDSDREYY
                     ALTQRGVESLEYFFNRIPTSVTEKIDKYIDSNKENLLADTQVKSSFVKQSDNEFIVNL
                     RVIENQSNLIDLNLNVSSEKQAKLICNNWKNNASYMYAEVIDLLIRDIH"
BASE COUNT         2470 a          589 c          984 g         2102 t
ORIGIN      
        1 tataaagaaa tttaaatttg aaagctagtg agtacagttt ggaaattgaa ataaagtaaa
       61 ctaaatgata ttttagattt ggaggtgata ttatgttcag ggtagttata tgtgatgacg
      121 aaaaaataca aagaagcata ctaaaagagt ttacaaaaaa acttttgtct gaaagatgtc
      181 taaattatca aatactggaa ttttcctgtg gagaagattt aatatccaaa tacccagaaa
      241 aaattgatat tatattttta gatatccaaa tgaaagatat caatggaatt gaggtggcta
      301 aaaaaataag aaagtttgat gaaaaagtag aaattatatt tacaacagct ttttcagagt
      361 atgcccccca aggttatgag gttcgtgctt atagatattt agtaaagcct atagagtata
      421 gtaactttgc aatgggtgta aatctttgta ttgataacct tcaaagaaag aaggagcgtt
      481 atatagtttt aaatagtaaa aaaggattta atagaatact gatagattct attttatatg
      541 ttgaaactgt aaagagagat ttagttgtac atacaatagg tagagattat aaagctaata
      601 tgagcatgaa acaagcagaa aaattactgt gtgaaaatgg cttttttaga tgtcatacaa
      661 gttttattgt taatttaaga agaatagaag atcttaggga taatatgatt actattaatg
      721 aacaatttat acctataagt aagtatagac ttaaagattt aaaagtagca ttaacaagct
      781 tgttatgtga tataatgtat taattttaaa aaggtaattg aaaatatcgt aaaaaagatt
      841 taatgtcatc acagggctat tgattaaaac tgtcttaata tataaaatgt tatttatatt
      901 aagacagttt tattatgcaa aatatcagtt tatagacttt taatattgtt taatatgtat
      961 atgtagagtt tccattacag tagcatgatt attaattagt agcctgatta ttaatatact
     1021 attattgatt tttatatgca ttatatgtat ttttagaata tgattacctt tttaaaataa
     1081 tgactattta taaaactcta tagatatttg tttcatttag ctatgcatac tttaattaat
     1141 tatattaata tttaatggag tataatataa atattaaagc gacatcttat agtttattga
     1201 acctatagta atgaattaat aaatggttta attctatgaa aagatttcct tttatgcaat
     1261 ttatttattg aaatatgata taatatataa aggtgtaagt attagttgtt aaaataatat
     1321 ttatattagt ttttatgcat acttggtata agtttttcat atattggtta aaattgatgt
     1381 tgatttatta tttttagtat tttttcacct tttgattcat actttgtaga atggtgtatt
     1441 ttataaaact gattataaga caagttcatt caaattagca tttttatatc atataaataa
     1501 ttgttccccc taaataagat ttagatgtat agtattaaaa tcaaaaagta taaaataaaa
     1561 ttgatgctta atcatagata aatattaata aaatatacaa ggggattgtc taataaaaag
     1621 ataatctcct tttttacaaa taatcctaaa ttgtgatata aatctaaaaa ttttatctta
     1681 acatttaacc atttaatgat aataattgat ataataaata taaattaaat atatggtgta
     1741 cttattttat gtttttagca agaaatatag catttaaaag ataaattgtg caacttaaat
     1801 caaagtttaa gatgaactat taacatagat atattaaaaa cttttgtaat ggagaatata
     1861 tgtgatatga ataataaagt taaggttgca gtattagata caggaataga caaagaacat
     1921 gattacttaa aagataattt agttggagga attgcttttg aatgtatgca tgattatatc
     1981 tttatatcag ataaatttga tgatgaagat ggtcatggaa ctgcttgtgc atcaataata
     2041 aagaaagagt atgaagatgt agagttattt gtaataaaaa ttttgagaaa agatggtatt
     2101 acaaatataa aagttcttga agaagcatta agataccttt tagacactaa cataagatta
     2161 attaatttaa gtttatcagt cataggagta gagagtgtta agggcttatt tgaagtttgt
     2221 tacgaattgt ttagaaaagg aaaaataata gtatgttcac tcgctaatga ttttgattta
     2281 agttatccag ctatgtttaa caatgttata ggtgtaagag gatttacttt agatggagat
     2341 gattcatttt ggtacaacaa aaaatatgat gttcaatgtg ttatggatag caattcatat
     2401 ataagttgtg atataaataa ttcctataga ttaccaccaa aatgtaatag ttatttatca
     2461 gcaaagctta ctggtaaaat agctaagata ttatcagaaa aaccaggcat aacaatatct
     2521 gatttaaatg ataagcttga atcattagca actagaaatc attgggatag ttgtgatttt
     2581 gatgagtgta gtagaattgt aaactttaaa ttggatttgt atgacaaaga aaatgctctt
     2641 cttatgaaag ttgctgacgt tgttagggat tgtttagata ttgaagataa taatgaaaaa
     2701 ctctttaagg gtagcctttt taataaggag ataggtttgg tttatgataa ttgttttaat
     2761 ctgttgaaaa agctagagca tagatttgac ataaagttta attatatgga catctcaaaa
     2821 tatgacttag tttcaatata tacactgact gaattagtgg aaagatataa aaacttataa
     2881 ataaagattt gtagctaaat ttaatgtaga aggtattaaa ttatatgtat tctttgtcgg
     2941 tagattaaaa gtaaaaaata tactataatt agatagcata agagaaaatg ccaatacagg
     3001 gggagttaga taatgaaata tgaagaagca ttagagtaca tatcacaaac taataaattc
     3061 ggtataagat taggattaga aaatatcgga aagttattag aattattagg aaatccacaa
     3121 gaaactttga atataataca tgtagctgga actaatggaa aaggttctgt gtgttctttt
     3181 gtttctaata tactaagaga gtgtggatat aaagttggtt tatatacatc tccatattta
     3241 gaaactttta ctgagcgtat aagagtaaat ggacaaaata taccacagga tgatgttgct
     3301 agaataattg agttaataaa agaaaaaata gagattatgg taaaagaagg ctatgcatat
     3361 ccaacagaat ttgaagtggt tactgcaatg gcattttatt actattcaga acaaaaggta
     3421 gactttgtgg cattagaagt tggtttaggt ggcagatatg atgctacaaa tataataaca
     3481 aaatcacttg taagtgtgat agtatctata agtttggacc atacaggtat acttggagat
     3541 acaatagaaa aaatagcgta cgaaaaggct ggaataataa aggaaaatgg agtagtacta
     3601 gtatatgacc agactgatga agcgaaggat gttataaagt cagtttgtaa agagaaaaaa
     3661 gctaaatata tagaagttga ttttgatgat ataaacataa aaaaatcaga tataaattct
     3721 caaatatacg attgtactgt aatgaaagaa acttatagag accttgaaat aaaattgata
     3781 ggtgaacatc aaataaacaa ttctatcttg gcaattagtg taataaaata tctaaaagac
     3841 ataaataaat tagcaaatat aaatgaagaa tctataagaa aaggacttat aaatactaaa
     3901 tggccaggaa gaatagaaaa gataaaagaa aatccaattt ttataataga tggtgcacat
     3961 aatgaagatg gagcaaaatc tttggcaaaa gcacttgata agaattttaa aggtagaaaa
     4021 ttaaccttat taataggtat gttagaagat aaagatattg atagtgtttt agagatattg
     4081 ttgccacatt ttaataaggt aataacaaca acaccaaata acccaagggc cataaattca
     4141 gatatattaa gagaaaaagt attaaaatat gtagatgatg taacatcaaa gcatgagata
     4201 gaggatgcag taaattatac attagaaaca tcaagtgagg atgatattat aataagtgca
     4261 ggttcattat atatgattgg tacagtgaga actttagtaa agaaattata attttataaa
     4321 ataaatatac aaataaagat ataaaaaaga gttgtctaaa aaatggacaa ctctttttat
     4381 ataaagtaag tttttaataa ttttactgat gtttatataa gtaattatat agattgacgt
     4441 atagccttag ataattggtc tgggcaagaa gtaccttttc caccacagtc aataccgttt
     4501 aatttatcag caacttcaag agcattttgg ccgattatta agcttctaag tcctgataaa
     4561 ttaccattac atcctcctat aaactcagca tcaacaatca cattattttc atcaacttca
     4621 aaaatcattt ctctacaaca tacaccactt ggattaaatg ttactttcat taaaaagacc
     4681 tccattaaat attatttctt tttttaacaa taataataat accaaatata tgcgtaataa
     4741 tgcaactata aagcataata caagctaatg aaataatata ttattgtagt atatgtattg
     4801 tagagaggag gggttatatg aaaatctata tttctaacta cttatcaaag agtattagca
     4861 tagttgatta ttctacactt gaactagaaa aagaaatagt attagaagat aatatatatc
     4921 cacaccattt ttgcatagaa aaagaaaaaa atttaatata catacctagt tcaagtaatg
     4981 ggatacttta tgtcttagat ttaagtaatg ataaaataat tgatactgtc tctataggag
     5041 gaagtcttag tcaagttgta cttagtgata atgaattgtt tgtggcaaat gaagattcta
     5101 atagtatata tgtactagat aaaaacacat taaatccaat aggaattatt ggagttgata
     5161 atatgccaca tggatttgac tttgatagag aaagtaaaaa attatatgta ccgtgtataa
     5221 actcgatagt atgtatagat acaattaata aaaatataca aaagaaaata aatatggact
     5281 ttagagcttg gcatatagag cttgataaac aaaagaaaga aatgtatata tcaacacttg
     5341 atggaaagct tataataata gatgaagtaa gcatggatat aaaaaaggta ttatatgaat
     5401 ttttattacc agtagaaata agatttaatt atagtggcaa aaaagtttat gttgctgatt
     5461 tgggttataa taatgtaaga attttagatt atacaacgag tagatacatt ggaaatattg
     5521 aaataaatgg gattcctcaa ggcttagaaa tatctaaaga tgaaaagcta ctatttgtat
     5581 cagatacaca agaaaattca gtaaaagttt atgaaactgc aaacaataaa ttaataaaag
     5641 aaatcaaggt aggaaaagag cctacaacta taatttgttt gtaggcttta atgtatgtct
     5701 cttataagta aatcgataac ttcggcatac atataagatg cattattttt ccagttattg
     5761 caaataagtt ttgcttgttt ctctgatgaa acatttagat ttaaatctat taagttagac
     5821 tgattttcta tgactctaag gttgacaata aattcattat cactttgttt tacaaaacta
     5881 gactttactt gagtatctgc tagtagattt tctttgttag aatctatata cttgtctatt
     5941 ttttcagtaa cactagtagg tattcgatta aagaagtatt ctagactttc tacgcctctt
     6001 tgagtaagag cataatattc tctgtcagag tctttatatg tagtcaaaaa cttagattcc
     6061 ataagctgag ataaaagctg ttgtaatgaa aaataattca tcatctcagt ctctaatact
     6121 acttgagtta tttgagaatt agtta
//
LOCUS       DACTGW010000208         6122 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.208, whole genome shotgun sequence.
ACCESSION   DACTGW010000208 DACTGW010000000
VERSION     DACTGW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.208"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(132..2726)
                     /gene="clpB"
                     /locus_tag="I9573_003068"
     CDS             complement(132..2726)
                     /gene="clpB"
                     /locus_tag="I9573_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAT4797665.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSDDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(2763..3359)
                     /locus_tag="I9573_003069"
     CDS             complement(2763..3359)
                     /locus_tag="I9573_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797666.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(3376..3780)
                     /locus_tag="I9573_003070"
     CDS             complement(3376..3780)
                     /locus_tag="I9573_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="HAT4797667.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNICFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            4147..4521
                     /locus_tag="I9573_003071"
     CDS             4147..4521
                     /locus_tag="I9573_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4797668.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            4527..5381
                     /locus_tag="I9573_003072"
     CDS             4527..5381
                     /locus_tag="I9573_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797669.1"
                     /translation="MDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGKTTT
                     LKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIMSKI
                     YKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATSSLD
                     PIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDELID
                     TMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRNPNCIVDNTSIEEIM
                     LFYVRGDK"
     gene            5381..6019
                     /locus_tag="I9573_003073"
     CDS             5381..6019
                     /locus_tag="I9573_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAT4797670.1"
                     /translation="MKGLILKDLLNLKGNVKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         2023 a          924 c          707 g         2468 t
ORIGIN      
        1 ttaaatttaa tttggacata ttgacttaaa aatgttttat taatttgata aaatatttct
       61 tttcctatag tttataaata aaaatcagct gagcaaaata tttagtatat tatgctcaac
      121 tgattctttt attatttaac taaaacttca atattttcat cttttccatc tataactata
      181 gtacatccat tatacacatc tccagcaata agtttctttg caattatagt ttctaatgta
      241 tttcctatat atctcttaag aggtcttgct ccatatacag ggtcatatcc ttctctcacc
      301 ataacatctt ttgctgaatt tgtaacttca atagtaatat ctttttcttt taatctattt
      361 tttaaatctt tcataaatat atctataatt ttcttgatat tttcttggtc taaaggctta
      421 aacattataa tatcatctac tctgtttaaa aactcaggtt taaatctatg cttcatttca
      481 ttcattacta aattgttagt agtctctgtt atatttcctc ctgcttctaa tagataatta
      541 cttcctatat tagaagtcat tattataaga gtatttttaa agtctactgt cttaccttta
      601 ttatcagtaa gtctaccatc atctaatatc tgtaagaaca tgttaaatac atcctcatgt
      661 gctttttcta tctcatcaaa tagtattaca gaataaggag cacgtctaac tgcttctgta
      721 agttgaccac cctcttcata tcccacgtac cctggaggag gtccaactag tcttgataca
      781 gcatgttttt ccatgtattc agacatatct attcttatta tattatcgtc actatcgaat
      841 aaatttctag caagagtttt tgcaagttca gttttaccaa cacctgtagg gcctaagaaa
      901 ataaatgaac caattggttt tctttcatct tttaatcctg ctcttgcacg tattactgca
      961 tttgaaactg ctgtaactgc ttcatcttgt ccaataactc ttttatgaag ttcatcttct
     1021 agtttaagta atttttctct ttctccttca actagtttag ttactggtat acctgtccac
     1081 tttgatacaa tgctagatat ttcttcttca gtaacttctt cttttaaaag agaattttca
     1141 gaaccatcat tcatattttc ttcgtactgc tttatttgtt cttctaactt aggtatttca
     1201 ccatatttaa cctctgctgc tttgttgaaa tcatattctc tttcatactt ttcagcttta
     1261 cctttagctt cgtctaattg agcctttaag tttttaatat ctaatatttg actcttttct
     1321 ttttcatatt ttgctgtcat ttcatcattt ttagatttta actctgctat ttctttttga
     1381 atatcatcca atcttgattt gcttttatca tcattttctt taagtaatgc ttctctttca
     1441 gtttctaatg taaataactt tcttctaaca acatctaatt ctgttggcaa tgaatctatt
     1501 tcactacgaa tcatagctcc agcttcatca attaaatcaa ttgctttatc tggtaaaaat
     1561 ctatcttgta tatatctatg agaaagtttt gctgctgcta caatagcatt atcatgtatt
     1621 cttactccat gatgtatctc aaatctttct tttaaacctc taagtataga tattgtgtct
     1681 tcaacagtag gttcttctgc aattacaggc tgaaaacgtc tttcaagagc cttatctttt
     1741 tctatatact ttctatattc atcaaaagta gttgcaccaa tacagttcaa ttcacctctt
     1801 gcaagcatag gtttaataag attacctgca tccattgaac cttctgtttt accagctcca
     1861 acgatagtat gaatctcatc tataaacagt ataatctttc catcagaact ttgaacttct
     1921 tttaaaactg cctttaatct ttcttcaaat tcacctctat attttgcacc agcaattaaa
     1981 gctcccatat caagtgagaa tacaatctta tcttttaaac cttctgggac atctcctctt
     2041 actattctct ctgctaaccc ttctacaatt gcagttttac ctacacctgg ctcaccaatc
     2101 aatacaggat tattttttgt tcttcttgat aaaattctta taatccttct tatttcttca
     2161 tctctcccaa ttacagggtc taatttattt ttcttggcta aatctactaa gtttgttcca
     2221 tatctagcaa gtgcatcata tgtgccttct gggtcttgtg tttctactct ttgactacct
     2281 ctcacttgtg ataaaatatt taaaaagtca ttcttattta tattatattg ttttaatatt
     2341 ttaccaacag ctgattttga ttctgtttcc atcattgcaa gcataacatg ctctacactt
     2401 atatatgaat ctttaaattc tttagaaata ctctctgcct ttataagcac ctcatttatt
     2461 tttctagtag ctgatactcc ttgtgagtca gcaccttctc catatacttt tggaagctta
     2521 tcaatttcaa agtttacagt atttctcaaa gaatcaattg atatattcat tttttcgatt
     2581 atatttggta tcaatccatc ctcttgatta ataagtgctg agaaaagatg aattacatct
     2641 acttgttggt tatgattttt tactgcttcg ttataagcct catttaaact tttttgaact
     2701 cttaaagtca ttttttctac gtccatataa atccctccat tttaatatta tatccaaata
     2761 aattaaattc tctcatttat aattattgat tttctaagct ctaaaagact atcaagtaaa
     2821 ttctcgtttt tttgtatatc gaaactcaat gttaaattaa atgttccatc tgaattaaaa
     2881 aaatcgaata cttcttttga ttttgctatt aaatacaagt cttcatctat aagtcttttt
     2941 tcacctatta ataaatactg acatttatct ataaaactct ttttaaaatc aatagtgtat
     3001 agattttcta aaatattata ttgataaata gcaatattat taaacacttt taatatgttt
     3061 gaatttacaa aattgtaaat ttgattattg ttatacagtg atatttgttc gtatatatat
     3121 tccctaaata aaggatgaat gttttgctta agttttaaag taacttcagc tggtatttca
     3181 aaataaagct tccagtactt agagttattt ttttctaaaa taaaagctac tttgttcaaa
     3241 ctggtagaac taatctcctt tagtgtacaa ttaaaattat agtgtctcaa actagttttt
     3301 tcattctcaa atacatagtc aaaccactgt agaacatttc tatcaagttt taaactcata
     3361 taaaaacctt cttccttatt tactaacctt attaggtctt tttatctgat tatattctgc
     3421 taataaactt tctttatcag ctctactacc tttatatgct atatgtggat ttatcatata
     3481 ctcaccattt ttcactcttt gtaaaaaatc catttttttc aactccgtaa acaatgaaga
     3541 taaagaagtt tttggatatt caaattcaca agataattca tataatgttt ttctaatctt
     3601 attattttta tccatagagt ttattaattt taatggaatt aaatccctct tagtatacat
     3661 tccttctact actttaatat aattcttaaa acaaatattc atatacttac ttttattcat
     3721 aactaaactt ttgctctctg tatattcctg atttaaatcc ttttttaatg tcttatccaa
     3781 attaaatatc tcttctcact ctccttatct ctaattgata atataaatat attacaaaaa
     3841 aatatgtatg tcaaaactga aaatgcaaaa agtttacctt ataagtaaac ttttgtttag
     3901 ctataagcta aactttatta tttttttaat ttagtatttc caatttattg gcaggatttt
     3961 tgaataatat ttataaattt tttaaaaaaa tttaaatcta tttaatatgg cagcctttca
     4021 taaaatacat tacatttatt aaatgtataa tattggtaat tagttttttc taaaatcagt
     4081 tgacatatta tatatatatt tatataatgt gtatatacta tatatacaat ttaaggaggt
     4141 gacttattga atatagttat tagtaattcg agtgggaagc caatatatga acagattact
     4201 acacaaatta aaagtatgat tataagcgga gagttaccag aaggctcagc tcttccaagt
     4261 atgagactat tggcaaagga acttagaata agtgtaatta ctacaaaacg agcatatagc
     4321 gatttagaac gagacggatt tattgagaca gttactggta aaggcagttt tgtatctagt
     4381 aaaaacatgg attttgtcaa agaagaacaa tttcgtttaa ttgaagaata cctacaaaaa
     4441 gcagttgata cagcaaaaag tagtgatatt acatatgacg aattaaaaga tattttattt
     4501 ttattatata aaggagaatg ataagtatgg atgcaataac tataaaaaat ttaaataaaa
     4561 cttataaaga ttttagccta caagatatat ctttttcagt accaaaaggg agtgtaatgg
     4621 gatttgtagg tgaaaatggt gcaggaaaaa caactacatt aaaagcaatt ttaaatttaa
     4681 tatcttatga tagtggaaat atagaaatat ttggtcttga tagtaaaaaa aatgaaaaag
     4741 aaattaaaga gcaaattggg gttgtatttg agggaagcaa ctttcatgaa aatttaaaca
     4801 ctgaccatgt ttcaaaaatc atgagtaaaa tctataaaaa ttggaatgac acattattta
     4861 aagactactt aaaaaaattg agagtaccag acaataaatt aattaaggag ttttcaaagg
     4921 ggaataagat gaaacttagt attgctgtag ccttatctca taaaccaaaa ttattaatac
     4981 tagatgaggc aactagttca cttgacccta tcgttcgtga agaaatacta gatatatttt
     5041 tagattttat acaagatgaa gaacactcta ttatcttatc ttcacatatt acaagtgatt
     5101 tagataagat agcagactat attacattta tacataaagg aaaaattgta tttagtgaaa
     5161 ataaggatga attaattgat actatgggag ttttaaaatg taaacctagt gattttgata
     5221 acctttcaag agaggattat tcatattatc gtaaaagcca atttggatat gaagtacttc
     5281 ttaaagataa acatagattt atatcaagaa atccaaattg tattgttgat aatacgtcta
     5341 tagaagaaat tatgttattt tatgtgagag gtgataaata atgaaaggtt taattttaaa
     5401 agatttactt aatttgaaag gaaatgtaaa gtttatactt ttattcatca ttatgtttgg
     5461 gtttatgtct tctttaggag atggaaatgt taataacttt ataggtgtta ttatagtttt
     5521 atgtactaca atgattgttt caacattttc atatgatgat ttaaataaat gggatagtta
     5581 tgtacttaca atgcctatta atcgcaatga tattgtatta agtaaatatt taactatgtt
     5641 aatatttagc tttataggtg ttcttgtttc acttattgtt agtgtaacta ttggttattt
     5701 taaaaataca cttatattaa atgaaacact acttattaat gcactaatct tatcaatttc
     5761 agtatgtttt ggtagcctta ttctaccact tatttataaa tttggtacag aaagagctcg
     5821 tttattaatg attttatgct ttttagttcc aacactagct ttattagttt ttaaaagtat
     5881 tttagaaaat attagttcac ctatttctat agaaattata cttaatacac tagtatattc
     5941 tcttccattt gttgctattt tactatttgt gatttcatat tttatatcat caaaaatcta
     6001 tagtaaaaaa gaagtataaa atatactata catgtctctg tatttaagta ttttaaataa
     6061 aatactatca agtttatttt ggtagtattt tattttttta ttattaatat ctactaggta
     6121 tt
//
LOCUS       DACTGW010000209         6110 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.210, whole genome shotgun sequence.
ACCESSION   DACTGW010000209 DACTGW010000000
VERSION     DACTGW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.210"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(586..1302)
                     /locus_tag="I9573_003074"
     CDS             complement(586..1302)
                     /locus_tag="I9573_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4797671.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(1843..2643)
                     /locus_tag="I9573_003075"
     CDS             complement(1843..2643)
                     /locus_tag="I9573_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HAT4797672.1"
                     /translation="MTFKNKKFTINKYEILSIILVLCLILTGFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNVGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(2958..3689)
                     /locus_tag="I9573_003076"
     CDS             complement(2958..3689)
                     /locus_tag="I9573_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797673.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(3676..4371)
                     /locus_tag="I9573_003077"
     CDS             complement(3676..4371)
                     /locus_tag="I9573_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT4797674.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(4373..5164)
                     /locus_tag="I9573_003078"
     CDS             complement(4373..5164)
                     /locus_tag="I9573_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797675.1"
                     /translation="MKNKFFTTLLTGFFAIVFFFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(5174..5986)
                     /gene="modA"
                     /locus_tag="I9573_003079"
     CDS             complement(5174..5986)
                     /gene="modA"
                     /locus_tag="I9573_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT4797676.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         2135 a          851 c          738 g         2386 t
ORIGIN      
        1 acaactatag atattaaaaa tactatagct aattttttat ttatagtttt tttattatac
       61 atactaagta agtttgaaaa gaatacatat gttatagaca tatcttttag attatagtta
      121 ttatatttaa tattttcata tattatatag actatagata taattataaa attgtagatt
      181 attaaattag tgttgtaaaa cattttatta cttgtaaatg catgaaacct taaggttaat
      241 agtatcaaag ttaatataag tgtaagttct atttttacct tagaaaaaag gcttatgaca
      301 ttctctaaga atttgacttt agtctgttta ttttttttat acaatgtaaa acatagtata
      361 gtcaataaaa aacttgataa tataatacct atgcagacaa actctaattc tacttttgac
      421 aaatgtacag gagtaccgaa aaaataaaaa taagggagag caaactcttt ataataacaa
      481 ttaatattat aagaaaagtt attattcaaa tcataaactg cttcaaataa tatagaaact
      541 aatagaaaca gtaataataa taaaataaat tttgctttag aatcattaat aattaagttt
      601 ttcaattttg taaccaatgc cccacaccac ctttaaatat tttggttctt ttggattaat
      661 ttcaattttt tctctaattc ttctaatatg aacagcaata gttctctcac aactataagc
      721 atcttcattc cagacatttt cataaatttc acgggaagag aaaattttac cttttcgtga
      781 cataagaagt gtaagtattt tatattcaag tggtgtcatt ttaatttcct caccatctaa
      841 agaaactatt tttgtatctg tatttagctc taggccacca ctttttagaa tataattagt
      901 atcatttgag ttatttgtat tactaaaatc acctaaattt atataccttc taagttgaga
      961 ttttactcga gcagtaagtt ctagtggatt aaatggttta gttatatagt catcagcacc
     1021 catattaaga cctataattt tgtccatatc ttctgatttt gcagataaga gaattattgg
     1081 tatattcctt ttttctctta ttttaagtgt agttttaagc ccatctaaat tgggcatcat
     1141 tacatccatt attattaact taatatcatt ttcgattagt atgtccaatg cttcaagtcc
     1201 atcatatgcc tttaaaacat taaagttatc aggtctcaaa tagaactcta tagcatcaac
     1261 tatctctcta tcatcatcta caaccaatat attttgattc aatttattaa cccccttgaa
     1321 attttatttt taaccaattt ttattttata ccaaaaagag gtattagaaa acagtaagcc
     1381 cgttcattat gaaataaaaa ctagtacttt taatatattg tcaatgcata tagtaaattt
     1441 aaaagtatag acgcccatat taatctgaag gtaaaagttt atgaaaatat ctagggtaat
     1501 ctatcacact ccttatgtta ttttgggttt attaattttt taggtttaaa catatattaa
     1561 caaatataaa taacaaatga gtttcataat tattaagaat ttataacaaa tatttatttt
     1621 aatgtagttt aatataaata ggtagtttaa tgtagatatg tataaagtat ttaataaggc
     1681 ttataggttt taatgatgat atctttatat attttaagag atgatgaaat tagatatggg
     1741 taatagttga atgagaaaaa gtagtatagc acaaaaagtg ctatatcaca aaatctcaaa
     1801 taatttcctt cttataactt aaattaaaag aataaatatt tcttataaat taggtgtatg
     1861 aagtatagca ggaacatctt tattatttaa taacttatca aatgatttat ggtcagactt
     1921 atagccaata ccttctctat attctaattc atcaacaatt ttgtattgtt gacaggcatt
     1981 tttaaccttt tcatagtctt cagtagaatg agctttataa actatcttat tattaaaagt
     2041 tattttatca gcaaatttag gttttacaaa atatttagca tcttttattt taccaacatt
     2101 atataaattt tggataacag aaccattttt taaatgttgt aaatggtcat ctaacttata
     2161 agtatgtgct atatgagtta catttttaaa tttttcttca aatgtaagag caattttttc
     2221 catgtaattg aagtcagtat taccatttgt atttgtttct ggtaataata ttatattttc
     2281 ccttattaca ccaaggctat taagagaagc taaaaattct ctatttctat attctgattt
     2341 ttgtatattt gttaggtttt catatttttt atatttatca aagacggata taccacatcc
     2401 agtagatact aaaactacaa atacatgatt ttttcctctt tcttttatag cattaacaat
     2461 agcactccca gcccacaaca cttcatcatc ttgatgctga acatagaaga caacataatc
     2521 tttaaatttt ttatgactat aattcacata gctactaaaa ccggttaaaa tcaagcataa
     2581 aactagaata atagatagaa tttcatattt atttatagta aattttttat tcttaaatgt
     2641 catttaatca tctcctgaaa agttgtctta gttatattat caaacaaagt actaaaaatt
     2701 atttcttaaa tgctgaaaaa tatagagtag attaatggaa taaaattttt tttattagtt
     2761 taaaataaag aaaacattta tgtataagac taatcaaaat attaataatt gataggaatt
     2821 gagatttaaa gggattgtac atttattcgg gattcatttt aatagaattt tactttagta
     2881 cttagaataa aagtaataac tttttaaatt taaagagtac acatatatat ttgtgtactc
     2941 cttgatgata tgattgctta tttactacaa aaacagagtc tttttaaacc taaaatattt
     3001 gatgcaccag ctatcgtagt tccataatag taaactggtt tgttaagtga tagaaaattt
     3061 ataattgagt tattacataa tgtacttcct gtgcaaatca ctaaatcagc ccaagatatt
     3121 acatcttcat aatctcgtat accatcttca atcttaacat tatattgaat agtatccaca
     3181 aattcaggat ttaaatctaa tactcttgtt tcgaaaaagt ccttaatatt atcaattatg
     3241 gctggttgat atcctataat tgctactttt ggattaccaa attccatttt tataaactca
     3301 gggaattttt ttgcacatac ctctggctcg ttatttttac aatgaatggt tctatccgtc
     3361 ttgcctaagt atttcataac tgcatttaat gatgctataa aaagacttct atcatgagga
     3421 ttgtcatgta aatctaatga taatatgtct tttaaagaac cttcaaatgt agatggagta
     3481 gaagtaaatg cttgtccttt agctccttta aagtcagctt caagcattat ttctttacca
     3541 actataattg ggaaatcttt acgcttagta tttccaattg cttcttttga agatagagat
     3601 ttggacacaa tacttatcat atccatattt aaattatatt tatcgacttg tactaataga
     3661 taatctctca aaatatcaaa tatattcata gaaaaaagat tctcctttat tttttatttc
     3721 ttcatttaaa tcaaaaatat gatttatttt tccattgttc attattccta tcttatgagc
     3781 aaaaaacatt gcttcagaaa tatcatgagt aacatgaatc actgttggtt tgtattttat
     3841 gtagacatcc ttcaaaattt tgcttatatg tttttttgta atataatcca atgaactcat
     3901 aggttcatct aaaagtaata tatcaggttc agctattaag gcacgtgcaa aagcaactcg
     3961 ttgcttttcg cctccactta aatttttagg atacctattt tttatatctt ctatctgcat
     4021 taatttagac aagtggttag ccttaagaat ctgttcatct ataggtattt ttcttaattt
     4081 aagtggaaac cttatattat cttcaacatt catatttgga aataattcaa aattttgata
     4141 tacaaatcct atatttctat tttctggagg taagtttgat attaaatttt ctttgtatga
     4201 tacatttcct ttagcagatg gatatcttcc tgctaatgtt tctaggaata gagttttacc
     4261 tgacccacta gctcctaata aaacaaagta ttcatgatta ttaatattta aactattttc
     4321 ttgtaattta aaattaggta aatcaagaga taaatttttt atttttaaca tattaattct
     4381 tatcaacctc tctattaaaa aatatctcaa aaagcatcaa gcatgtaagt gagataaata
     4441 tcaaaattac agctattccg attgcaatgt ctatatcacc agtagacata tttaaatata
     4501 tagatgtagg aataatttca gttttcatac gtgtagaacc tgctatcata gctactgcac
     4561 caaattcacc aagtgctctt gaccaagtca ttacagttgc agatatgatt ccagttttta
     4621 gatttggaag aattataaat ctaaatgctt cccaactatt acatccaagg gtacgtgcaa
     4681 caaatatcat cttttcatta ctttcattta gtgaagtgtg aagtatttta atagtaagtg
     4741 gaagatttac aaatacttgt gctaaaacta taccttgctt tgtaaatata aaatcaagct
     4801 taaaaatctt ataaatggaa tctccaatcc ccattcttcc aaacaaaagt aaaagagcaa
     4861 ttcctgatac taagtgtgga agagacatag ggagatttat tatctgtata atcagttttt
     4921 taaatggtaa tttagtaata tgtaaaaaat atgctactgg aatagctaat aaaaggcata
     4981 ctatagtaga tattaaagat gttttaatac taagtgttat tgcatactgt acttccacgg
     5041 attttaagca tataggaaca taacttatcc ctttaatgat taacataaga agtggtgata
     5101 caataaaaaa aaagactata gcaaaaaatc cagtaagtag tgtagtaaaa aatttatttt
     5161 tcaagtttaa tcactactta acaggttcaa aaccatattt tttaaatatc tgtttagctt
     5221 catctgactc tatataattt ataaatttat tagctaaatc ttttttatca ctatagttta
     5281 cgattcctgc tgatatgagt tggtctatat ttttatcttg ggcaatttct ataacttcta
     5341 catcttcatt gccaaagacc gaatcttttg tggctattgt agcatcagct ttacttgatg
     5401 ttaactgtac aatcatttca tttacagttg cagctgtaga gacaacatta tcatttatag
     5461 attttaaatt atttttttca agtatttttt gtgtagtctt acctatagca ttagactctt
     5521 tgtctcccaa aataagttta actccatctt tatttaagtc ttccaatttt tgaatattag
     5581 ctggatttcc tttaggtaca actattgcag gtgtatgatg tgcaaccgtt ttatgttcac
     5641 ctacaagttc tttttcaaag gcctttgaat aaattggttc agaaccaaca ataaatacgt
     5701 ctcctttgtg acttgtctca atttgagcta ttagctgtgc agaacctgca tagctatatt
     5761 caactttgac attattttct ttctcaaatt tttttgctat ttcatccatt ggtttcttta
     5821 aacctgcccc tgaatagaca aaaagagtct ctttttcttt tgaatcatca gctgttgtca
     5881 ctttagtatt agattcatca ttagctttgt tttctttaga attacatgca gtaatactta
     5941 atgaaataat catagctaaa gctagtactg aaagtttttt aaacataaga tattccccct
     6001 tagtaaatta gtgtaaattc ttttttagta tatcatacta agaaaacgat gtctaagttt
     6061 ttgttatgtt ttataatgtt ttgttatgtt ttattatgta tagttaagaa
//
LOCUS       DACTGW010000210         6021 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.211, whole genome shotgun sequence.
ACCESSION   DACTGW010000210 DACTGW010000000
VERSION     DACTGW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.211"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(11..259)
                     /locus_tag="I9573_003080"
     CDS             complement(11..259)
                     /locus_tag="I9573_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797677.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            525..2654
                     /locus_tag="I9573_003081"
     CDS             525..2654
                     /locus_tag="I9573_003081"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAT4797678.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            complement(2911..3510)
                     /locus_tag="I9573_003082"
     CDS             complement(2911..3510)
                     /locus_tag="I9573_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HAT4797679.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(3528..>6021)
                     /locus_tag="I9573_003083"
     CDS             complement(3528..>6021)
                     /locus_tag="I9573_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAT4797680.1"
                     /translation="LSNNINIKKEYNIFDSKEEKKKERETLKILNEEEKNYEELLKYK
                     TIWENKEGFTYEYRPGQYELTKTIRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAIL
                     EARINKKRLLISTDTKELQIQLINKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLE
                     AYIDDYESQNPTKGEILSLIFLKRLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCD
                     PNMCRPKKCKKDCLYKNRIEELKEEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLT
                     EKGYDFFSSIINSKSLRYLLQEIYPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKI
                     GREDKQKIARSINLIIEEIDSILKFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDG
                     IDTEISYRAYSEKIKLSCEKIIKNLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDL
                     EDIKIIFEIFLEYDEKDDYARIVEIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIF
                     LSATLSLSESMSYFKNTLGIDRVKNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPN
                     EMSKIISNISKITEGHTLALFNSKDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDL
                     NRKCVVLGSKGCFEGVDIPGDGLVCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYP
                     QMTIKVKQAMGRILRSKYDYGCFVIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTY
                     IRRHLNKSRSLILKSVIFDTIKALNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVY
                     HIDIIKKDMKVKYFDRNYLINLDIFMREEDKN"
BASE COUNT         2058 a          956 c          682 g         2325 t
ORIGIN      
        1 tttacataaa ttatgatatc tttcttttta tatccctaga atgactcttt tgttttttat
       61 tagtactgac agttttcgtt cttgtcttgt gtatagcttt gctatcaaat atttttctag
      121 cattgcataa aacaattcca attgttaaac aaactgtaag cacacttccc atcaaaaagt
      181 aataatccaa tcctatatgt actcccataa ctgaatttat taacactaat gcaaaagtca
      241 ttgcaaaaca tatcatcatg gagataacta aaattcttag aatttttttc aatccaatca
      301 cctcgtataa tcactaaaat tgacactacc ccttatccat tactatacca aattagtaaa
      361 aaaatagcaa ttactattgt catttatatt aggaaaatat taaatctttt taataaatat
      421 ctttttttga cataaggatt acttaacttt taataaaata gtaacactaa tatgtattaa
      481 ttaatagaat aaaataaatg ataatttatt gaggggtgat ttacatgcca aatttgccaa
      541 gtttagggtc aaaggctcct gattttaaag ccaatacaac aaatggtcct attagactct
      601 ctgactataa gggtaattgg attgttttat tttcacatcc tggtgatttt acaccagttt
      661 gtactacaga atttttatgt tttgctaaat attatgacga atttaaaaaa agaaatacag
      721 aactaattgg tctaagtgtt gatagtaaca gttcacattt agcttggatg tataatattt
      781 ctttacttac aggtgtagaa attccatttc ctattataga agatagagat atgagaattg
      841 ccaagttata cggcatgata tcaaaaccaa tgagtgatac atcaactgtt cgctctgtat
      901 ttattataga taataatcaa attctaagaa cgattcttta ttatccacta actacaggaa
      961 gaaatattcc agaaatactt agaatagtag atgcacttca gactagtgat agagataata
     1021 tagttactcc tgcaaactgg tttcctggaa tgccagtgat tttaccttat cctaaaaact
     1081 ataaggaatt aaaaaataga gttaacagtt gtaataagaa atattcatgt atggactggt
     1141 acttatgttt tgtaccagat aattataatg atgaagaagt gagcaagaaa attgataata
     1201 cttgtagctg gaaaaaagaa catactaaaa acattgaaaa tgaatgtaac tgtgaacatg
     1261 aacatcatga ctacctgaac aaagctcttg actgtaaaca agaacacaag actgatatta
     1321 aagatgattg caaccatgag aaaaaacata ctaaaaatac taacaaagtt cacaactcca
     1381 aacaagataa gtttaaagat aagtcttgtg atgaaatgaa ttttaactat gataaagatg
     1441 aatcttgcga caaaataaat tctagctata acaaagaaga tagtagttat gaagatttct
     1501 ataaacataa ttataaaaac tacgattata ctagcgaaaa aaatactaaa aaaatagcta
     1561 tgaaaacttt aaaagattca aaaaaattag ttagaccaca aataacagac ccatacaatc
     1621 caatagttga aaatgcaaac tgtccagata taaatccaat tgtagcagaa tatgttcttg
     1681 gaaatccaac taatgtagat gctcaactat tagatgcagt tatatttgct tttgctgaga
     1741 tagaccagtc tggaaatttg tttattcctt atcctagatt tttaaatcaa ttacttgctc
     1801 ttaaaggtga aaaacctagc ttaaaagtaa ttgtagctat tggaggttgg ggagctgaag
     1861 gattctctga tgcagcatta acacctacat ctagatataa ttttgcaaga caggtcaatc
     1921 agatgataaa tgaatatgct ttagatggaa tagatataga ctgggaatat cctggaagta
     1981 gtgcatctgg aataacatca agacctcaag atagagaaaa ctttacactc ttactaactg
     2041 ccataagaga tgttatagga gatgataaat ggcttagtgt agctggaaca ggagatagag
     2101 gatatataaa ttcaagtgct gaaatagata aaatagctcc tataatagat tattttaatc
     2161 ttatgagtta tgattttaca gcaggtgaaa caggcccaaa tggtagaaaa catcaagcaa
     2221 atctttttga ttcagactta tctttgccag gatatagtgt tgatgcaatg gtgagaaatc
     2281 ttgagaatgc tggaatgcct tctgaaaaaa tccttctcgg tataccattt tatggaagat
     2341 taggtgccac tataacaaga acttatgatg agcttagaag ggattatata aataaaaatg
     2401 gatatgaata tagatttgat aatactgctc aagttccgta tttagttaag gatggagatt
     2461 ttgcaatgtc atatgatgat gctttatcaa tattcttaaa aactcaatat gttcttagaa
     2521 attgtctagg tggtgtattc tcatggacat caacttatga ccaagcaaat atactggcta
     2581 gaaccatgtc tattggtata aatgaccctg aagtattaaa agaagaactt gaaggtattt
     2641 atgggcaatt ctaatagaat gtaggaagat tttatagtta tataaaaagc agattttatt
     2701 atattaaata agttgtatct aattaatact attattaatt gtttataaat tagatactta
     2761 ttttttcata atacaatctg cttttttaat caaaatttat ttaattataa gttgtttaat
     2821 tacaagttgt ttaattacaa gttgtttaat tataaagtag gaatttagat atactcttat
     2881 accatgtaat tatgtataaa tattttttta ttatacacca atagcttttt tagctaattt
     2941 atcagcttcc tcattatact tatttccaga atgagcttta acctttgtaa attttacatt
     3001 aagcttattc ttaatactgt catagaaatt tttatatgct atagttcctt ctttatttgt
     3061 tttccaaact ccagtacacc acttttcaat cccttcatag tcaaagtata aaatcaagtt
     3121 ttgaacatta ttgtcaatac agtactgcat tgcaatttta gaagcctcta tctctccagc
     3181 tacatttctc atacttacta aagttttctc ttttcctttt tcactataag tttttataac
     3241 ttcattattt tttaaaatca caacacccga accatacatt tttactgaat gttcataact
     3301 tccatctaca tatgcttcta taaactcctt atcttcttga aaactcaatt tttctgaccc
     3361 atcaatatat tctttagctt cttgaaaagt agaaaaactc ttatattcag ctcctgagaa
     3421 tccatttact tgttttttgc actcatccca tgtattatat acacctatat ttttaccttt
     3481 tttcacagca taaaactttt gttttgccaa atatctcact cccactctta atttttatct
     3541 tcttctctca taaatatatc taaatttata agatagtttc tatcaaaata ttttactttc
     3601 atatcttttt ttataatatc aatatggtac acttctcctt ttacagctct ttgtcttata
     3661 ttttcattta tatctttttt tataatatct ttatcaacat cattgttatc cattttagca
     3721 tcaacattca acgcttttat agtatcaaat ataacagatt ttagtattaa acttctagat
     3781 ttatttaaat gtcgccttat ataagtataa aactcattgc tattaacctt tgaaatttta
     3841 caatcgtgta aatctttctc taatctcttt aaaacactta tattagttcc tacatcaaaa
     3901 atcacaaaac atccatagtc atatttgctt cttaaaattc tccccatagc ttgctttacc
     3961 tttatggtca tttgaggata atttatagta tagtagtcta taccatactt cttcattata
     4021 gtaaaataca aagggtcttt tggatttaga ttcggaagct tgtctaaagt tacacaaacc
     4081 aaaccatccc ctggaatgtc tacgccttca aaacatcctt ttgagccaag tactacacat
     4141 tttctattta aatcgttaag gtgtctaatc ccctttttat cagcataaat ttctaagtta
     4201 aaactatgta agtatttttt caatatttca taagtttttt cttgtctatc tttactatta
     4261 aaaagagcta atgtatgacc ttctgtaatc tttgatatat tagatataat tttactcatc
     4321 tcatttggaa attcagaatt tctgtactca caaatatcag aaaatccaac tacagagact
     4381 ctatttttat aatcaaaaat tggttctata atcttttcca cattcttaac tctgtctatt
     4441 cctaatgtat ttttaaaata agacatactt tcagataaac tcaaagttgc tgataaaaat
     4501 attccttttt ctaattgaga taaaatattt tcttcaaata aatctgctat ttttaatggt
     4561 actactcgaa attcaaagtc attataattc ttatcaattt caactatcct tgcataatca
     4621 tccttttcat catactctaa aaagatttca aatataatct ttatatcctc taaatctctt
     4681 gtctttgcct ttccaaattt atatatatct gcttctttat ctatactatc atcatcaata
     4741 ttcctataaa ttattattat tattgaaact aaatttttta ttattttctc acaactcaac
     4801 tttatctttt cactataggc tctataactt atttcagtat ctataccatc cttttttagt
     4861 tttcccacaa tttcatctat ttgcaaattc agttcccatc ttaaattata attacttaca
     4921 ttattatatt cattacaatt accaaacttt aatattgaat ctatctcttc aataatgaga
     4981 tttatacttc tagcaatttt ttgtttatct tctcttccaa tttttaatac attataaaat
     5041 ttatcaaaag cctttatttt tctcatattt ctactatatt ttttatatat aaagctacta
     5101 ttttgaataa actcgtaagg ataaatttct tgtaataaat accttaaaga ctttgaattt
     5161 ataatgcttg aaaagaaatc atatcctttt tcagtcaaat tatgtgcttc atctactatt
     5221 atattttcta aaggtttttc atccttatac ggccatttag ctaatagtga atgatttact
     5281 actgttatat gttcttcttt taattcttct attctgtttt tatataaaca atcttttttg
     5341 cattttttag gtctgcacat atttgggtca caacttacat ttcttaagtg ggtacttatt
     5401 tccttgaagt tgtcaaacac taaataattt atttcttcta tatctccata cttgccacct
     5461 tcaactaatc tcttcaaaaa tattaaagat aaaatttctc ctttagtagg gttttgagac
     5521 tcataatcat ctatatatgc ttccagtcta tctatacaaa tatagttatt ttttcccttt
     5581 atatatccgt aacttacttt tccatttaga cccaaagaat ctaacacatt tggaatatct
     5641 ttatttatta attgtatctg caattccttt gtgtcagtag aaattaataa tcttttttta
     5701 ttaattctag cctctaatat tgcaggcaat aaatatccta cacttttacc tataccagtt
     5761 ggtgcctcta tacatgcaat tttttcttca tcttcgctat ttctaaacaa ctctcttatt
     5821 gttttagtaa gctcatattg accaggtcta tattcatatg taaatccttc tttattctcc
     5881 caaatagttt tgtattttaa taattcttca taattttttt cttcttcatt taaaattttt
     5941 aaggtttctc tttccttctt cttttcttct tttgagtcaa atatattata ttcttttttt
     6001 atatttatat tatttgataa a
//
LOCUS       DACTGW010000211         5984 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.212, whole genome shotgun sequence.
ACCESSION   DACTGW010000211 DACTGW010000000
VERSION     DACTGW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.212"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..787
                     /locus_tag="I9573_003084"
     CDS             <1..787
                     /locus_tag="I9573_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAT4797681.1"
                     /translation="KLYTDMNDFIENVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIV
                     RPKKDIICLGLNYKDHVNEIPDGVIKNVVMPDYPIYFSKRADKIIGVDDKISLHGDLV
                     EKLDYESELAVIIGKEGINISKEDAYEYIFGYTIVNDISERALQDKHVQWFRGKSLDT
                     HTSMGPCIVHKEEFEHPLKLDISSVVNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKP
                     GDIISTGTPAGVAMGMNPQVYLKHGDIVECKVEGIGVLKNIVD"
     gene            886..2061
                     /locus_tag="I9573_003085"
     CDS             886..2061
                     /locus_tag="I9573_003085"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HAT4797682.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEELLKDIPTANKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            2189..3043
                     /locus_tag="I9573_003086"
     CDS             2189..3043
                     /locus_tag="I9573_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797683.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSNESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKIKDDSSC
                     LYLDIYND"
     gene            3087..4322
                     /locus_tag="I9573_003087"
     CDS             3087..4322
                     /locus_tag="I9573_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAT4797684.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREIVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKSISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNEKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            4423..5364
                     /locus_tag="I9573_003088"
     CDS             4423..5364
                     /locus_tag="I9573_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HAT4797685.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(5593..5946)
                     /locus_tag="I9573_003089"
     CDS             complement(5593..5946)
                     /locus_tag="I9573_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAT4797686.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMIW
                     LEKLKEDNTYEIYSI"
BASE COUNT         2299 a          599 c         1097 g         1989 t
ORIGIN      
        1 taaattatat actgatatga atgattttat tgaaaacgta tctacaggag atttagaaaa
       61 aataaaaaac aattcatttg aaaacgcaaa gtgttataag ttagaagaag ttaaattatg
      121 ttccccaatt gtaagaccta aaaaagatat tatatgttta ggtctaaact ataaagacca
      181 tgtcaatgaa atcccagatg gagttataaa aaacgtagtt atgccagatt atccaattta
      241 tttttctaag agagcagaca aaataattgg agtggatgat aagataagct tacatggaga
      301 tttggtagag aagttggact atgaatcaga acttgcagta atcataggta aagaaggtat
      361 aaatatatca aaagaagatg cttatgaata tatatttgga tatacaatag taaatgatat
      421 aagtgaaaga gcacttcaag ataaacatgt tcaatggttt agaggaaaaa gcttagacac
      481 acatacatct atgggacctt gtatagtaca taaagaagaa tttgaacatc ctttaaaatt
      541 agatataagt agtgtagtaa atggagaagt aagacaaaat tctaatacag agtactttat
      601 atttgatata cctactataa taaatgattt atcaagagga atgactctaa agccaggaga
      661 tataatttca actggaactc cagcaggggt tgctatgggg atgaatcctc aagtatattt
      721 aaagcatggc gatatagttg aatgtaaagt cgaaggaata ggagttttaa aaaatatagt
      781 tgattaatgg ataaatatgc catataattt tattagtaca aataatttaa ttaatatagg
      841 attgatcaaa cttatgatta atttattata gagagggatg agtatatggc aagagaacta
      901 gaattaaagt atggctgtaa tccaaatcaa aaaccatcaa agatttatat gaagaatgga
      961 gaacttccta tagaagtatt aaatggaaaa cctggatata taaatttttt agatgcattt
     1021 aatagttggc aacttgtgaa ggaattaaaa gaagctacag gtcttccagc agcaacttca
     1081 tttaaacatg taagtccagc aggagcagca gtaggggtac ctttatcaga tactttaaag
     1141 caaatatatt ttgttgatga tttagagctt tctccattag cttgtgctta tgcaatggca
     1201 agaggagcag atagaatgtc ttcttatggt gattttatag cactttcaga tgtatgtgat
     1261 aaggagactg caactattat agcaagagag gtttctgatg gtataatagc tccaggatat
     1321 acagatgaag cccttgaaat attaaaagga aaacgtaaag gcaactataa tattgtaaaa
     1381 atagatgaaa attatactcc tgagcctatt gaaacaaagg atgtttatgg aattactttt
     1441 gaacaaggta gaaatgaaat attaataaat gaagaactat taaaagatat tccaactgcg
     1501 aataaaattt ttacagatag tgcaaaacgt gatttaatta tagctcttat tacattaaaa
     1561 tatacacaat caaattctgt ttgttatgct aaagatggac aagtaatagg ggtaggtgca
     1621 ggtcaacaat cacgtattca ttgtactaga cttgcaggta acaaagctga tacttggtat
     1681 cttcgtcaac atcctaaagt attaaactta aaatttaaaa aggatatagg tcgtccagat
     1741 agagataaca ctattgatgt atatctttct gatgattata tggatgtgct tgctgatggc
     1801 atatggcaaa acttttttga agaaaaacca gagcctctaa ctggagaagg aaaaagagca
     1861 tggcttaaaa ctttaacagg agtagcactt ggttcagatg catttttccc atttggagat
     1921 aatatagaac gtgcaaaaag aagtggggta agttttatag cacaaccagg tggttctatt
     1981 cgtgatgata atgttatatt aacttgtaat aagtataaca tagtcatggc atttactaaa
     2041 aatagattat tccatcatta aaatactgaa agtataaaga taatatagat taagtaaaaa
     2101 tctacaacaa gttttgttgt agattttttt atttgctgta taattttaaa tagaacttta
     2161 tttttagata ttcaatttgg aggttgatat ggctttaaat ataagaaact atagcctatt
     2221 atgtaatcaa ggaacagcta aatttaatga agatgtagtc ggtataaatc catttggggc
     2281 atgggtttta gatggtgcta caggacttaa tggaaaaaat ttagtgtcaa atgaaagtga
     2341 cgcaaggtgg tatgttcagt ggtggaatag gtatttatat aaaaatataa ataaagaaga
     2401 atcattaaag agtattatta atactggaat aatagaagta aaaaaagagt atgaactaag
     2461 attaaatggg tttaaggttg aaaaattaga tttaccatct agttctatag ttgttgtaaa
     2521 atttcatgaa aataaagtag agtatttttt attaggagat tgtgctttgt ttattaaaaa
     2581 tggagattcc aaagttataa aagataggag tatttgtaaa tttgataata ctgtatttga
     2641 tgaaatgagt aaactttcta acttggaaga gatggcattt gatgagatta gaactagtgt
     2701 tatggataca atagtaagca atagattgaa gaaaaatacg aaagagggct attggatatt
     2761 ggattttgat gaggaagctg tggaaaattc tatatatgga tacattgaaa taaaaaaaga
     2821 ttttcaattg atgctaacga gtgatggttt tacaagtgca tgtgatagat ataatctaat
     2881 aaaagaagaa gaattgatac gtatagcaga aaaatttggt ataggatata tacataataa
     2941 agtaagggat tttgaagata atgactataa agcggttaaa gtgccaagat ttaaaataaa
     3001 agatgattca tcttgtttat atttagatat atataatgat tagactgtat aaaactattt
     3061 aattttagaa aggaggcaag tgaaaagtga aaattcttca tataataaca caaaaaccaa
     3121 atagtacagg tagtggtata tatttgtctg gtatgataaa agggttcgaa aaaatcggtc
     3181 ataagcaagc tgtaattgca ggtatagatg taaatgatga tgtaaattgt tttccaagtg
     3241 aagtaagttt ttatccagta aagtataatt gtggggagtt aaattttcct gttgtaggta
     3301 tgagtgattc tatgccatat gaaagtacaa gatataaaga tttaaatata gacatgataa
     3361 atagattgaa atatcaattt aaagtaaata tagataaggc tatgaatgat tttaaaccag
     3421 aacttataat atgtcatcat ttgtacctct taactgcatt tgttagggaa atagtaaaag
     3481 atataaaggt tatgtctatt tgtcatggaa cttgtttaag acagttaaat accattgatt
     3541 tagagaaaga atacataata gcaaatataa ggaaattaga tttaatattt gctcttcatg
     3601 aaaatcagaa atatgatatt ataaagactt ttggagtaag tgaaagtaaa gtagttgtaa
     3661 ttggtagtgg ttataatgat gatatatttt ataataaaaa ttataaaata aaggatgata
     3721 aaataaaaat agtatttgca ggtaagatat gtaaatcaaa gggattgatt ccatttataa
     3781 aatctatatc taaattaaaa tatagtaaag atttaataga agtcaatttt gcaggtactg
     3841 gaagcgatat cgaatcatat aatgagatag ttaaactggc aagtaagtct ccatttaaaa
     3901 tgaatttcct aggaaagtta gaacagagag atttagctga gctattcaac aggtcacaaa
     3961 tatttgtgtt accatcattt tatgagggat taccagttgt agttttagaa gctttatctt
     4021 gtggcacaga tgtaatcact actgatattc ttggtgttaa agagtggatt ggtagtgaga
     4081 ttaataattc tggaaagata gaatatattt ctctaccttt tatggagaaa gaaggtattc
     4141 caaaagatga agagttatat gattttgaaa ataatttgta taatgcaata gattctaaga
     4201 tacaatcttt attgaataac tcaaatgaga aaacatctgt agatatgtca aaaaaaacgt
     4261 gggatggctt agcatataga ataaatgaag ttattttaat ggatgaattg tgtttagtct
     4321 aaaattattt aaattataaa aaagtgtaac tatttttaaa ctttttttaa atgattttat
     4381 attatactaa tactaaatat atttgtttta gggaggtaat ttatgaggat tagtaaaaag
     4441 gtattggcgt taggtatttc agcattactg ttaagtgtta gttttccaac atcaataaac
     4501 gcactagata agattgaaaa tattcaagga gtagataaat atgaaacagc tgggcttatt
     4561 tcagataagc aagattttac aacagcagtt cttatcaatg cagattctac aatggtagat
     4621 gggattgctg caagtggtct tgcaggagta aataatgcag ctattttatt aactaataag
     4681 gatgatatac cagaagcaac acttcaaaga ctgaatagag ttactaagat atatgtaata
     4741 ggtggagaaa attctataag taaagatgta gaaaaaatgt tacttatgag aagaatgcaa
     4801 gttataagaa ttgatggtgt agatagagta gatacaagtt ataaaattgc aggtgaaata
     4861 gaaaaaatta aaaacagtga taagatgttt ttagtaaatg gatttaagga tgaagctgat
     4921 gcagtaagtg ttgcatctgt tgcttataga gatggagctc ctataatatt gacaaaagat
     4981 ataccttcag cagaagaaga cacagactta gacccatggt ttggagtttc tcctgttcca
     5041 gtatatgcta ttggaggaga gtctacatta agtgattaca tagtgagcag atatagagca
     5101 actagaattg gcggtgttga tagatatcaa actaataaaa atgttattga aaagttctat
     5161 aatggagcaa aagagttcta tataacaagt ggagacgatt tagtttatgc tttagttgca
     5221 tcaccacttg caaaaaatgc tcctgttgtt ttagtttcaa ataaaagtga taagagtata
     5281 ttaagtggag ctagtaaagt aacagcaata ggtataagtg ataagagtat tatagagcaa
     5341 tgtttggatg ctgtaaagaa gtaatggagt gttattagta aaatgaatat atacattgaa
     5401 aattagtaga ttgttcattt tataatggta taaaatatat gggaagaggc tatcaaatgg
     5461 agataagcct cttttttatt gataaaatta tgaaaatata ctttatttta tataaaaaaa
     5521 gtatctcatg aactaaatgt ctattttgat atactatctc cttttcttat attatatgta
     5581 ttatatgatt ttttatatgg aataaatttc ataagtatta tcttccttta atttttctaa
     5641 ccaaatcatc tctaaaatat ctagttcttc taaatagtct ttctctgttt tgttatcatc
     5701 atgttttata agttctaaaa cttcaaatgt aaatgctttt tcaccatatt ttttccattc
     5761 attatttaat tcttttatca tacatgagcc agcaccaagc tgaaatttga gagagttaaa
     5821 tttgcttttt atgtcattac tttttccaag gtaaatcttt ttagtaatat cattttttat
     5881 tatgaataca cctgtgtcaa atttcatttc tttgtataat tgttttaatt cacgttttct
     5941 atccatgatt ttagatacct ctctttataa ttgatatgta atat
//
LOCUS       DACTGW010000212         5821 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.213, whole genome shotgun sequence.
ACCESSION   DACTGW010000212 DACTGW010000000
VERSION     DACTGW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.213"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            133..2352
                     /locus_tag="I9573_003090"
     CDS             133..2352
                     /locus_tag="I9573_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT4797687.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYALNQDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKITISVPLLNNDK
                     VVGVLYCTYNINTLMKLINVSFYENNSISYVVRNDGTIILHPQEDSLSKNIYKLLKQD
                     NDIREINRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSEQIINRAIYAVLFIILIFIAIIFYIIYIKKSNEKEILSLAYEDKVTHIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGKNKRIRKIDSIRNNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSINLSRVELYQPDIVKFINKTIQMYNLSSDLIEIEITETVAINE
                     LNILKNVLNELRKYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLNGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            2606..3268
                     /locus_tag="I9573_003091"
     CDS             2606..3268
                     /locus_tag="I9573_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797688.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            3638..5047
                     /locus_tag="I9573_003092"
     CDS             3638..5047
                     /locus_tag="I9573_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAT4797689.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     SLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGGIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            5162..5437
                     /locus_tag="I9573_003093"
     CDS             5162..5437
                     /locus_tag="I9573_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797690.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DITVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
BASE COUNT         2381 a          531 c          992 g         1917 t
ORIGIN      
        1 aatgttttta tggttattag gttgatttta tgtgtaacat ttaagaatag ttatatacaa
       61 tataaatgat gaatcggtat aaaaaggata tattatctat atgaagtatt gactaattag
      121 gggaggagtc gaatgacagg gagaaataaa agttttacat taattacaat tcttttttta
      181 acatcattat ttttgacaat ggggtatatc tatatagaag atacaaaaca tcttcttatg
      241 tcagaggcaa aaatacatat aaaagaagtt gcagtacaag gctctcaact agcacaaaga
      301 caaattgaaa aagatttaga tgttttaaat attttttcta actattatgc tttaaatcaa
      361 gatataccta atgaggaaaa aatgaaaaat cttttagatg aaatggaaaa tcaaaaattt
      421 tacactatgg caatagttga tataaatggt aatgctgaaa ataccaaagg tgataagttt
      481 tcagtgaaag atagagaatt tttcaaaaac tcaattaaag gtaagaagta tgtttcaagt
      541 ccatatgttg atgaagtaaa taagtctata aagaagatta ctatttcagt accattatta
      601 aataatgata aagtagttgg agttttatat tgtacttaca atatcaatac tctgatgaaa
      661 cttattaatg tatcttttta tgaaaataat agtatatctt atgttgtaag aaatgatggt
      721 acaattatcc ttcatccgca agaggatagc ctatcaaaaa atatttataa attgttaaaa
      781 caggataatg atattcgaga aataaataga ttaaaaaaag aattgcaaga aaataaaaca
      841 ggtgcaactg tattaaatat gttggaagaa agaagatatt taggatatgc tactatggat
      901 aatgggaata gtgaaaataa ttatataaaa gattggaatt tgattttttc aattccagaa
      961 actgttgtat ttagtaattc agaacaaata atcaacagag ctatatatgc agtgttattt
     1021 attatattaa tatttattgc aattattttt tatataattt atataaaaaa atccaatgaa
     1081 aaagaaatat taagtttggc ttatgaagat aaagttaccc atataggaaa tcaaaataaa
     1141 ttttatagag agtgtagtaa gtatctttta gataaaccat ctttaaatta tattatagta
     1201 tattttgata ttaataactt taagatgatt aatgatactt ttggatatga atttggtgac
     1261 aatttattaa ttacgatagc aaaagcttta aaagaagaat taacagaagg tgaagtatat
     1321 gcacgacttt caagtgatta ttttgcaatt ttctgtgatt ataaaaatgg aaaaaataaa
     1381 cgtataagaa aaattgatag tatacgaaat aatattgaaa gtaatttgag tatagtattt
     1441 gaaatttctc tgtgtgtggg tatttatttt gttgaagaag gtgaagtaga tatccaaaag
     1501 gctgtaaata aagcaaatat ggctaggtct gttgctaaag gtaaaaatat aaattatgct
     1561 atttataatg aagatgttag gaataagctt agtgaagaat caatgatttt agatgatata
     1621 aaaatagcat tggtaaaaaa tcaatttgaa gtgtattatc aacctaagtt ttcacttgta
     1681 actggagaaa tgatagggag tgaagcacta ataagatgga atcatccaga acatggtttt
     1741 ataagtcccg cagtatttat tccaatagca gagaaaagta agttgatttt aaaaattggg
     1801 agatttgtat ttgaaagggt ttgcaatgat ttatctgaat ggaaaaagca aggtaaaaag
     1861 atagttccag tttcaataaa tttatctaga gttgagttgt atcaaccaga tattgtaaaa
     1921 tttatcaata aaacaataca aatgtataat ctaagttcag atttaattga aatagagata
     1981 acagaaactg tggctattaa tgagttaaat attttaaaga atgttttaaa tgagttgagg
     2041 aagtatggat tttctatttc tatggatgac tttggtacag gatattcttc tattagttgt
     2101 ttaagagata tgccaattga tatattgaaa ctagataagt cttttcttaa tggtattgaa
     2161 catgatgaga gaagtcgcaa tatagcaaaa tcaatagtgt ctctagcaaa atcactagat
     2221 ttagttgtaa ttatagaagg tgttgagagt aaagaacaag ctgagttaat gaaacaattt
     2281 ggatgtgatt tagttcaagg attttatttt gcacgaccaa tgccagctaa gaatttttta
     2341 gatttacttt aaaacaaaat ttaattttta tataaagcac tattatgaat gtttgatttt
     2401 aataaatcac tgctaaataa ttagtgtttt tttattttat caatgtattg tttaatataa
     2461 agcaaaaaat aattgtattt tataaaaatt atattaaaaa taaatttatt ttgctaataa
     2521 acatgttatt aacaaaagaa tagtactata atgtatagat gataaaaact ttaataaaaa
     2581 tttagtgtat aggagttgtt taagtatgat agatgtgagt attgatgaca aacttaagga
     2641 aagatggcct aatgctaagt tgggttgcat tcaagcaaaa gtagcagtaa ataaaagctc
     2701 agaaaaatta attaatgaaa taaatgagtt ttgtgatact ataaatcaaa gtctaaaagt
     2761 tgaagacatt acgaaactag ataaaattaa agatgcaaga aatgcttata aagagcttgg
     2821 aaaaaatcca agtagatata gaacatcttc agaagcattg gttagaagaa ttattcaagg
     2881 gaagggtctt tatacaataa ataatatagt agaaataaac aatttaatat caataaaatc
     2941 attatatcca gtaggtacat ataatgtaag caaattacat tcaccagtat gttttactgt
     3001 aggagatgaa ggagaacagt ataagggtat tgggaaagaa ttaataaata tagaaaatct
     3061 tcctatattg tctgattcag tgggtaaatt tgggagtcca actagtgatt ctgaaagagc
     3121 aatgataaca gaagatgcaa atgaaatatt aatttgtata ttttctttta gtcaagaatc
     3181 tgatattgaa gaatattttg gatatgctaa ggagttactt aaagaatatg ctgatggaaa
     3241 agatatagaa actaaaataa ttaaatagaa tctatataac aacgttatgt taagctaaaa
     3301 ttcaatataa aaataagtta agataaagaa actaacataa aattagtttc ttttttaaaa
     3361 gagtagttta gtgttacaaa aacagataga taacccacga ttattaaaag tatggtaata
     3421 tattaatata gcagagattg tatttaaggg attttgaaag gggtgattta taatagtcat
     3481 tttgtctaaa agataaaatg gttttatgga aataactata tatttaaaaa tataaacaat
     3541 gatacagtaa tttgggtata taaaataagg catacatggt tttgtttaaa aaccaataaa
     3601 gtacataggt aattacaatg taaggaagga tttaattatg attgatggaa aagaggtaaa
     3661 acaagaaagt ggacaaaaaa atctaaaatt taagatagct atagttgtag gtgttttgtt
     3721 tgtagcttat ataggaatgg caatcttttt tagaaatcat ttttactttg gaacgactat
     3781 taatggcgta aaggcttcag gaaaaacagt tgagcaagta aatgatttgt taacatctag
     3841 tactaattcc tatactttaa acttagagga aagaaacaat aagaaagaac aaataaaagc
     3901 aaaagatatt gatttaaaat ttacaccaga tgataaagtt caaaagataa aagatagtca
     3961 aagcgctatt ggatggattg gtggaatatt taaatctaaa gagtataata atatgataac
     4021 tctttcatat aataaagatt tactagaaaa aaggtttaat gctttatctt gttttgatag
     4081 taaaaatgtt gtagaaccta agagtgctta tcctaagtat gatagcacta aaaacgctta
     4141 tgttatagta ccagaggtac ttggaaacaa agtgaaaaaa gatgattttt ataagttagt
     4201 acaaaactca attaattctg ggaatacttc tatttctctg gatgaaagta aagtttatga
     4261 agcaccaaac aacacaaagg attcagaaaa gttgaaacaa gctattaaga cattagataa
     4321 gtatgttggt gtagtcgtta catatgattt tagtgaccgt aaggaaactt tagatggttc
     4381 tgaaattaat aaatggttaa aagtagactc agaaaagaat tatgacataa cttttgataa
     4441 tgatgctatg gtagaatata caagaagttt aagtaaaaaa tacagtactt ttggtgactc
     4501 aagaccattt aaaactgcca caggtaaaga tattacaatt tcaggtggaa tttatggttg
     4561 gttgatagat aaaaagaaag aagctgaagc attagttgat gttgtaaaag ctggtaaaaa
     4621 tgttacaaga gaacctattt acatacaaaa agctgtatct cgtaataaag atgatattgg
     4681 aaatacatat gtagagatta gtctttctgg ccaacatatg tggttcttta aaaatggaga
     4741 aatgcttgta tcaacagatg ttgtaacagg cgatttagct agaggatttg ctacaccatc
     4801 aggtatatat cctcttaatt ataaggctag aaatgtttca ttaactggtc aaggttacag
     4861 ttcacctgta agttattggc ttccatttaa tgggaacata ggcattcatg atgctacttg
     4921 gagaaattct tttggtggag gtatatataa gtcttcaggt tcacatggat gtgtaaatac
     4981 acctttttct aaggcaaaga aaatatatga aaatatagaa ccaggtacac caattatttt
     5041 atactagtaa atgtgttgct aacagttaag cgaagtcatc ttagtttatt taggatggct
     5101 tcctttttat ataaagctta ttgaagttta cagaaagact acttaaaagg agaaatacat
     5161 aatgaatgaa aaattaacta aaattttaga agaagctaag aatgatgatg aatttagaaa
     5221 ggaacttata aaaacaagag aacaacgtga tgttatggat tcattttgta aggttgttac
     5281 atctagaggt tatgatataa cagtagggga acttttttcc atgggagaag aatatacatc
     5341 aaacctactg aaaagtgtaa atggaggagc agcatatcct attgaagatt ggagtgattg
     5401 gtacgaggat ttttttataa tgttgtcgaa tatatagagt aactaatgat attatctact
     5461 taagattaat acattgcctt aaaataaaac tattagaact aagtgtaagt tttatcaatc
     5521 tttaaaaata ttgatttatt tctaactata tataaaaaat ttagattgaa aataaggtga
     5581 caaaaatcga tatagcttga tgatttataa aagttagata aaatagtgcg agatatttct
     5641 aaaaattcaa aaaaaacaat aaacttcaag ggttagaggt tgaaaaagaa tataaaaaac
     5701 ttttatatgt aataaattaa attaaaatta taaaaaaaat aaaataaaaa tttaaaatgt
     5761 cgacatagcg ttaattttaa aacaaaataa cgctattgac aaactatatc gctttaaatt
     5821 a
//
LOCUS       DACTGW010000213         5776 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.214, whole genome shotgun sequence.
ACCESSION   DACTGW010000213 DACTGW010000000
VERSION     DACTGW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.214"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1332
                     /locus_tag="I9573_003094"
     CDS             <1..1332
                     /locus_tag="I9573_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAT4797691.1"
                     /translation="KYYNTGKTRDISFRIEQLKKLKLVVSQNEEKILLALKKDLNKSN
                     FEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQPYGVTLIMSPW
                     NYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVENTFSKEYVAVVQGSQEE
                     SEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCIILKDANIDLTAK
                     RLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPCNNEQFPKIINQ
                     RHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEIFGPIFPILTYK
                     DLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVTHIATNYLPFGG
                     IGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVLKKIM"
     gene            1471..2637
                     /locus_tag="I9573_003095"
     CDS             1471..2637
                     /locus_tag="I9573_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAT4797692.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2754..3536
                     /locus_tag="I9573_003096"
     CDS             2754..3536
                     /locus_tag="I9573_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAT4797693.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3655..4308)
                     /locus_tag="I9573_003097"
     CDS             complement(3655..4308)
                     /locus_tag="I9573_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT4797694.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4332..5696)
                     /locus_tag="I9573_003098"
     CDS             complement(4332..5696)
                     /locus_tag="I9573_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HAT4797695.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2167 a          744 c          881 g         1984 t
ORIGIN      
        1 aaatattata atacaggtaa aacaagagat atatcgtttc gtatagaaca gttgaagaaa
       61 cttaagttag ttgtatcaca aaatgaagaa aaaatacttt tagcactaaa gaaagattta
      121 aataaatcga attttgaagg ttttatgaca gaggtaggca tgttttatag tgaattaaac
      181 tttgctataa agaatataag aaaatggagc aaaataaaaa gagttaaatc aagtatggtt
      241 aattttccat ctatatccaa agttgttcca caaccttatg gtgttactct cataatgtca
      301 ccttggaatt atccctttca attagcactt ataccattag tttggtcatt agcagcaggg
      361 aattgtgtta tattaaaacc ttctgaatat tctgtgtcaa catctagtgt agttaaagac
      421 atagttgaaa atactttttc aaaagaatat gttgcagttg ttcaaggaag tcaagaagaa
      481 agtgaaaagt tactattgga aagatttgat tatatatttt ttacaggaag tactaatgtt
      541 ggtaaaataa tcatgaaatc agcatcagag catttaactc ctattacttt agaacttgga
      601 ggaaaaagtc cttgtatcat tttaaaagat gctaatatag accttactgc caaaagactt
      661 acttggggaa aactaataaa tgcaggacaa acctgcgtgg ctccagatta tgttttagtg
      721 catgaagata gaaaaaatga gttaatagag aaaataaaat attatactaa taagtatttt
      781 ggagataatc catgtaataa tgagcaattt cctaaaatca taaatcagag acattttaat
      841 cggatattat ctcttattga taaagataag atagtctatg gtggaaatta taataaggag
      901 acattaaaga tagagccaac tatagtagac aatgttaatt gggatgataa tataatgaag
      961 gaagaaatat ttgggcctat atttcccatt ttaacatata aagatttaga tgaagtaata
     1021 caaaaaatta tacaaatgcc aaatccattg gcactttata tttttacaaa aaataagtac
     1081 ctagaaaata aacttttaga aatgattcca gcaggcggat gttgtatcaa tgatacagtt
     1141 actcatatag ctacgaatta cttacctttt ggtggtattg gtgaaagtgg tatgggctca
     1201 tatcatggaa aagctggatt cgatacattt actcactata agagtgtgtt aaaaaaatta
     1261 aatttggatg tccctattag atatgctcca tacgataata aattaattaa ggttcttaaa
     1321 aagataatgt aggttatatt gtagaataat aaatataaat aaaattgagt atttgtattc
     1381 tgaatatatt ttatttttaa tttggtatat ttgttataaa agtggtgatt ttgtgataaa
     1441 atttaattat atagaaataa ggaggaaaaa atgaaaatta attggaatac aaaatacaca
     1501 acaatagcaa tttatacatt tataatagct gctagtagta ttatatttta tctagtatca
     1561 tcacaaattg atgtattttc taataattta gatgctatat ttacaacatt acaacctttt
     1621 ataataggat ttgctattgc atatcttctt aattttattt taaagtttta tgaagacaga
     1681 atttttataa aaagtgagaa attaaaaaaa ctaaaacaaa gttcaaaaag aggcttgggt
     1741 cttttattaa catatgcaac agctacattg atactttact tatttatgca tttcgtattg
     1801 ccacaagtaa ttgagagtat tgttggacta gcaaatgata tacctatgta tgtaaacaat
     1861 gcaactaagc ttattgataa attaatgact gatttaaatc ttgatgagca atactttaat
     1921 ttggcagttg ataagtggaa tgagtttgtt acatatatta taaagtttgt aacagattta
     1981 atcccaatat taggaagtat gttaaagaat gtagcctcta gtatatggaa tattgtacta
     2041 ggattaattg tttcagtata cttattaatt gataaagaaa aattctatgg attaagcaaa
     2101 aaaataactt atgcagtatt tacagaaaaa caagctgcca gaattttaga attaactcat
     2161 agaagtaact atacatttgg aagattttta ggtggcaaaa tattagattc attcattata
     2221 ggtattctaa catttgtaat acttacatta gttaaaatgc catatacatt attaatatca
     2281 gttataattg gtattacaaa tattatacct ttctttggac ctttatttgg agctatacca
     2341 tctactctaa tagtgatgtt tgtatctcct ataaaagctt tctggttatt gttgattata
     2401 ttaataatac aacagataga tggaaatatt ataggaccta aaatacttgg agattcaatt
     2461 ggtatatctg cattttggat tttgttctcc ttattggttg caggaaaatt attaggtttt
     2521 attggaatgg ttataggagt tccaatgttt gcagtaattt attctataat taaggatatt
     2581 gttgaaagta aattggacaa aaagggatta ccgacagata cttccgatta catgtagcta
     2641 aaaaatatta tgtataatta aaaatatata aattaagcaa tatttaaagc actgagaaag
     2701 ttaaactctt ggtgcttttt agatataaaa ttattataga agaggtgttt tttgtggcat
     2761 ttatagaatg taaatttaaa tctaaatata ctggaggaga aactgatata attgtaattt
     2821 tgcctgtagc aaatggaaaa gaacttttta gtggtaaaga gatatatgat tttgaaggta
     2881 aatttaaaac attatactta tttcatggtt tagaagatga ccaaagctca tggattagat
     2941 atactaatgt agagcattat gctaaaaata aaaatatagc cctagtaata ccaagagtag
     3001 aaactagttt ttatacagat atggcgtatg gacaccaata ttttactttt gtaagcgagg
     3061 aattacctaa atttgttaga gctatatttc ctttatcaga taaaagggaa gataactttg
     3121 tagctggtat gtctatgggg ggatatggag ctttaaaatt agcactttca aaaccaaaag
     3181 agttttcggc agttgcaagt ttttctggtt cgcctgatat gattcatgag ttagaaaatc
     3241 aacaaataga aaccaatgca gacattttat ttcacgattt tggtagacca gaagacgcta
     3301 gaaatagtga gaatgattta ctttacatac ttaagaacct taaagaaaga aatgaagata
     3361 ttcctaaaat ttatcaattt tgtggtgata gagatttttt atataaaaat aatcagatgt
     3421 ttaaaaaata tgcagagtct ctgggtgtag atgttgaata tgaagagtgt gtaggggggc
     3481 atgaatggcg tctttggaat ttatggataa agaaatttat agatataatt gggtagtaca
     3541 cttatggtat taacaggtat actataaaat atctttaaat atgataaaga ctgaaatcct
     3601 aaaatcaaat tatgatttta aaatttcagt ctataagaat ttaataaaat tttattaagt
     3661 tcttacacga gttattattg gagtaaaatt gtcaactata tctatatagt atttactcaa
     3721 ttcctctaaa atctcttcgc ataaatccgt actatgacca agattaataa gaaactgttt
     3781 tgtaaaatgc tttattacag cttgagaaga tatatcgtac ttgtgtgaag tataaactct
     3841 aattatttca ggtttcatag caagtatacc aatacaagaa agtattaatt catcatgttc
     3901 tatagattct ttatcattaa catatctttt catgaccatt tcggttacac ttctttgata
     3961 gcttaaaata gcttcttcat aagctatatc caaataaaaa tttctatatg cttcgttttt
     4021 tagaaggaaa caaaggtcag cattcatagc aagaaagaac atcatcatag taccttcttc
     4081 atggtagtta tagattaaag actgcatttc atttcttata tcttcatata ttatagctcc
     4141 tatttcgctt ttacttgaga agtaatagct tattagacct aaatttatat tagctctttc
     4201 tgcaatttgc ctagttgttg tcttatgata accttgctca aaaaataatt ctctagcagc
     4261 acttataata ctagcttttg tttctttgcc cttttctgat tttgacatat ttactgtgta
     4321 tacctctctt ttcaatcttt ttttgaaaat ttgctaacta taaatattgt aatagaacca
     4381 cctattatag gaaccaaaat tctgtcaaat acagtaagaa taccaatcat atttaataat
     4441 gccaatataa cacttactat aacagcatag atagctcctt tttttgtagc gcctttccag
     4501 caaagaccaa ttacaaatgg tgcaaatatt acacctgtac ataatggagc cggccaaaac
     4561 aataattcta ataaactatc aaatttaagt gctatgattg cacctattat agctgtcaaa
     4621 gctgatgcaa tttgagaata ctttagagta ttttttaaat cattaaattc tatattagga
     4681 tgtaataatt tgttataaat atctcttgat ataatagcac tcattgaggt cattaatgaa
     4741 tcggcagtag acattatggc agccaatata gcagctaaga ataatacaga tattaaaggt
     4801 ggcaaagtag tcgatatcat ttgaggtaaa acctgaacag atgatatgtt tggaaataaa
     4861 ctaccaccaa tagctccaat tattcctata gggaaggcaa gaaataatag tataaatcct
     4921 gcaattacac tacttttaac tgcattttta gcattatcag cacattgata tcttaaaaaa
     4981 ctttcataag aaaatattga gcctaaaact aatggtaaaa ctgtatacat aaaatcacta
     5041 atcgatttat ttcctggaaa gaatggttct tttaccattg attgtaacat aagtaaaggg
     5101 ttgtcgccaa ctaattttaa gcttatataa gctactattg gcaaaaacac tatacaaata
     5161 gcaagttgaa taaatgctga tactgcaact ccccacattc ctgatgtata agtatatata
     5221 attataacta taactcctat aattacagct atatcataag gaataccaaa tgcagtagat
     5281 acagaaccaa aagaaattaa ttgactaccc ataatagcta gtatagatat tggtgttaca
     5341 agactttgat aagctcttaa aaatacacta tgaccatatc tagtttcaat ataatcagca
     5401 ggtgttatat cacaagataa gcttcttaat tttcctgcga aaaaggcatt tataaagcaa
     5461 gctaaagctg cacctatagt agaatacatt ccaacccaaa gtcctctttc agctccccat
     5521 tgaactccac caactaagca ccctcctcca aaagatgtag cagcaatggt tgcagctagt
     5581 ataggcaaag gaagatttct acctgcgatt aggaaatcgg atgctgtttt aacttttttt
     5641 gaaaaataaa aaccaatcca tatgtatatt gcacacgtaa caattaaaag taacatagtc
     5701 ttttctcctt tataaaatat tttaataaaa tatataaaat atatttaaaa tatattttat
     5761 ataaaagtag tacgat
//
LOCUS       DACTGW010000214         5674 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.215, whole genome shotgun sequence.
ACCESSION   DACTGW010000214 DACTGW010000000
VERSION     DACTGW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.215"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..598)
                     /locus_tag="I9573_003099"
     CDS             complement(<1..598)
                     /locus_tag="I9573_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAT4797696.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQR"
     gene            832..1284
                     /locus_tag="I9573_003100"
     CDS             832..1284
                     /locus_tag="I9573_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT4797697.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGIATNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            1287..1595
                     /locus_tag="I9573_003101"
     CDS             1287..1595
                     /locus_tag="I9573_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAT4797698.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            1709..2815
                     /locus_tag="I9573_003102"
     CDS             1709..2815
                     /locus_tag="I9573_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAT4797699.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            2890..5568
                     /gene="mngB"
                     /locus_tag="I9573_003103"
     CDS             2890..5568
                     /gene="mngB"
                     /locus_tag="I9573_003103"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAT4797700.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISNIENYIDSVKNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTANEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESSKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERSIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLLFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNNSKLKVNTKTVEVNSLSHCKVQTIVIK"
BASE COUNT         2270 a          705 c          906 g         1793 t
ORIGIN      
        1 ccctttgaag ctctattaaa tctacattat taaataattc tatttctcta tctgtaattc
       61 caattatata gttttcaaaa ttcctattta tcatattatt agaaatacat cttcgaatat
      121 ctatttcctt cccttcaact ttaactccat aatatggctt agaaactaat attaaattat
      181 actttgacaa aataggcttt attcttttta tgtcatttag tattgtaaat ttactcacat
      241 acaagctatt agctaaatct tctagtttta tatatgtatt gttatagaga atatatttta
      301 aaatatattg atttctttct ataggagaat ctggtatttc acttgaatcc ataatatcat
      361 cagatattaa tgctagatac tgttggtata atgctaaatt aagaatatct atataatatc
      421 cttctccttt ttttatttta ataatagcac catttttctc taaagttcca tttatagaag
      481 taatatcatt tcttatagtt ctctcactca cttttaaatg attacttaaa gttttacaag
      541 taattttatc atcactttca tttaataatt taaacaacgt gagcaatctc ttgtgcataa
      601 atatcccccc ttatagaatt tcttctaaat tatactattt acactcatat aatacaatta
      661 ctctaataaa attgtattat aataatttct ttccacttct atatggaaat tagttattat
      721 cataatataa atttccatgt cgaagtggaa attagttata tttaatttaa ttaggataat
      781 gattacaata taaatataag ttaagacatt acgaatggag tggaaaaata tatggtacta
      841 caagaaaaat atatatttat agataaagac ttaaaaagca aagaagaagt attaaatttt
      901 attgcagata gtgcttatat actaggtatt gcaactaata aagaggctct tttaaaagac
      961 cttctaaaaa gagaaaatga atttccaaca ggaataaaag atggatttgc gatacctcat
     1021 acaaaaagtg taaatgtaaa ggaagcttcc atactctata ttaaaacaac taacgagtta
     1081 gagtggaaaa ctttagatga tagtaaggtt agatatgtat ttagtttgtt agccccacat
     1141 aaaaatgaag gaaatataca tttaaaaact ctatctaaat tagccacatg tttaatggag
     1201 gatgatttta caagtgaagt taaaaatgct agtgatgcaa gtcatttagt aaaatatata
     1261 actaataaaa tgaaggagga ataattatga aaatagtagc aataacatca tgtatagcag
     1321 gattagcaca cacacctatg gcagcaaaag ctttagaaca agctgcatca aaaatgggtc
     1381 atgaaatcaa agtagaacaa caaggtgcta tgggacgagt aaatactata acaaaagaag
     1441 aagcacaaga tgcggacttt atacttatag catctgacca aactatatct ggaatggaaa
     1501 gatttgatgg taaaaaagtg ataaaagtca aaataggtat agcgttaaaa aaaccagaag
     1561 ctgtcttaac aaagtgtata gaagctattt cttaataaaa aaatctcact ctaatataga
     1621 atgagaaaaa taaaaaatta aaaaattgtc taatgtaatt ataacatatt atcaaaaaat
     1681 ataacaaact tatataggag gattaaaaat gagtaaaaaa aataaatctc ttttaagtga
     1741 acttaaggga cacttaatga caggtatctc atatatgttg ccacttgtaa taggctcatc
     1801 tttagttgta gctatcccaa agcttacagc tttagcaatg ggtataacaa gtttagatgc
     1861 ttataaaaat gtatctggtt tttatcatat tctttactta atggaacaag ttggttggac
     1921 tggcataggt ctattaaata cggtattagc aggatttata gcattttcaa taggtgagaa
     1981 accagctatg ggtgcaggtt ttattggtgg gctcatagca agtaatacta atgcaggatt
     2041 cttaggagca gtaatagcag gtttctttgc tgggtatgtt tgtaagtttt taaagaaaaa
     2101 aattaaaata tctgggtcag catcaggaat gatgccactt ataataatgc cacttataac
     2161 agttggatta actggttttt taatgagtgt tgtattagca ggacctttag gaaacataaa
     2221 tacttcatta aatgcttggg tagctgaaat gtgtcaaaat ggaacaaatt cggtggtatt
     2281 ggctttaatt ttaggcgcaa tgataggatt cgatttaggt ggaccagtaa ataaagcagc
     2341 ttggatggca ggaaatgcat tattgttaga gggaatctat ctaccagcga tattggtaaa
     2401 ctgcgcaata gttatacctc cactaggata tggtttagct acattcataa gaaaatcaaa
     2461 attttccaat gcattagctg aaacaggaaa aggcaatata gttatgggta taattggaat
     2521 aacagaaggt gcaattcctt tcactttaac aaatccatta aaattagttc ctgttaatat
     2581 gataggatgc gctttgggtg taggattatc agctttgtta ggtgtacatg ctataatgcc
     2641 tccagttggt ggattatatg gatttatatc aataggttct ggatgggctt acttagtagg
     2701 tgcaatattt ggagctttag taatagcaat attatcaaca actttagtag acttcaatga
     2761 agatgaaact acggaaaata cagaatttga tgaaatagag ttagatataa tgtaaattaa
     2821 aaacttaatt ttagataatt gagaattgag tgtactctca attctcaatt aaattttggg
     2881 aggattttta tgaaaaagac aaaagtacat gtagttccac acagtcattg ggatagagaa
     2941 tggtatttta caacatcaag gtcaaaaata tatttaatga aagattttca agatatatta
     3001 gacatattgg aagaaaaaga ggactttaaa tactttacta tggatgcaca agcttctctc
     3061 ttagatgatt atttaaaatg gagacctcag gatgaatata gaataagaga tttagttcaa
     3121 agaagaagat tagttattgg accttggtat actcaaacag accaattggt tatatcagga
     3181 gaatctataa ttagaaacct ttactatgga atagatagat gcaatgaatt tggtgaacct
     3241 atgagggttg gatatgtacc agattctttt gggcaatcag ctcaaatgcc acaaatatac
     3301 agaggttttg gaatagatag ttcattattt tggcgtggag tttctgatga tatggtagat
     3361 acaacagagt ttatatggaa aggttctgat ggttcaaaag tattagcagt tcaaatacca
     3421 tttggttatt actatggagg taatatacca gaagatgatt cagatttaaa agaatattta
     3481 aaagatataa ttggaaaact taaggttaaa gcttctacta ataacgtata ttttccaaat
     3541 ggatttgacc aagcaccaat aagaaaaaac cttcctgata tattaaaaaa agctaatgaa
     3601 atagataatg aaaatgaata cgaaataagt aatatagaaa actatataga ttcagtaaaa
     3661 aatgaaagaa atgaatttac agagcttaaa ggtgaatttt taaatgctaa acatatgaga
     3721 atacacaaat ccatattctc ctcaaggtca gaccttaaaa taatgaataa caaattagaa
     3781 aattatatca caaatgtact tgaaccatta ttaacattaa gttattcact tggaaatgaa
     3841 taccctcatc tagttataaa agatatatgg aaacttatgt ttgaaaatgc agctcatgac
     3901 agcataggtt cttgtaatag tgacacagca aatgaggatg tatacacaag atacaaacaa
     3961 gccagagatt tgagtataaa tttattagat ttgcatatga gacttataag tactcaaatc
     4021 aaaaataata atgaagaaat aacattaaca gtattcaatg gattcccaga agtcagaaat
     4081 gaagttgtag aatttgacac ttatattcca ggtcaagatt ttgtgataaa gaataaaaat
     4141 ggagaaattc ttaattatat aataaaagaa aaagaagata ttacaaatta tatattaact
     4201 caaacaataa gattaaaccc aagtaaaaaa atatatatac ccaaaactgt atatagagca
     4261 aaaattgcac tttcagtaaa agatatacca ccaatgggat atactcaact agtttttgaa
     4321 ttggatacta aaaataaatg tgaaatagaa acaagtaatt gcaaagaaat agaaaacaaa
     4381 tattacaaaa taacagccaa taaaaatggt tcattagaaa tattagataa agaatctagt
     4441 aagacttata aaaaccaagc tgtaattgaa gacaatggag atgatggaga ttcatacaac
     4501 tattctcctc caagaaaaga tatttatgtt tcatctttag attctgtatc ttcagtagaa
     4561 gtgctaaaat caaatattca agaagaaatg atacttaaat attcattgac cataccagct
     4621 tcactggaag aaaggtctat aggtaaagtt tctgtgaaaa tgccagtaac aatgaaaatt
     4681 accttagaac ctaaagaaca aataattaag tttaatgtta atataaaaaa taaccaagca
     4741 ctatctcata gagtaagagt tttatttaat acagaaatag catctaagtt ttctatagca
     4801 gatcaacaat ttggaataat acaaagacca acagtattag aaaaagacct tgcattatgg
     4861 aaaagagaca attactcatg gcaagaaaaa cctataacta tcgaaccaat gcaaagtttt
     4921 gttactttag aggatggaca aagaggaata gcattaataa ctggttgtgt aagagaatat
     4981 caaatagttg gtgataattt agatacaata gcattaacac tattcaggtc atttggatat
     5041 atgggaagag aaaatttact ttatagacca ggaagagctt ctggagaaaa aataatagca
     5101 acaccagatg cacaattact taaagagtta aactttgact ttggtcttta catatataac
     5161 aataaatttg atgaagcaaa tgtagctaat acagctaaga gattcttaac acctatacaa
     5221 atatatgaat atgctgattt tttaaatggt agacttctat ttgcattcaa tgacgaaaaa
     5281 caaatatatg aaaatgaatt tagtttgatg aatgtaaata attcaaattt cacagtaagt
     5341 gcaataaaga aagaagaaaa aggagatgga attgtagtta gaatcttcaa tggtatgaaa
     5401 aatgaggatg ctaagggaag tcttaacatc aataaaaatg tcaatgatgc ttatagtgca
     5461 atgctcgacg aaatctatga taataatagc aagcttaaag taaatactaa aactgtagag
     5521 gtaaattctt tatcacattg taaagtacaa acaatagtaa ttaaataata taaaatagct
     5581 tatacaagat ttcaaaataa attgctagta ctatgttaat agtaaaccca gatattatgc
     5641 tatttataag ctctgtaaca ctaaataaca tttt
//
LOCUS       DACTGW010000215         5663 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.216, whole genome shotgun sequence.
ACCESSION   DACTGW010000215 DACTGW010000000
VERSION     DACTGW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.216"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            16..2082
                     /locus_tag="I9573_003104"
     CDS             16..2082
                     /locus_tag="I9573_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HAT4797701.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYVWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINKLKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNICILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSSMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            2099..4054
                     /locus_tag="I9573_003105"
     CDS             2099..4054
                     /locus_tag="I9573_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HAT4797702.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKEAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDR
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLERSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            4070..5260
                     /locus_tag="I9573_003106"
     CDS             4070..5260
                     /locus_tag="I9573_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAT4797703.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
BASE COUNT         2047 a          693 c         1134 g         1789 t
ORIGIN      
        1 aatttaaaat aaaaaatgaa agaggtggta gagagtttga aaaaaattac taaagaaagg
       61 atagaaaata ctatgaaaaa gctatttacg agcgaaacat ttttggaata tcaatttata
      121 agtgattgcc aaatatcacc agatggagga tatacagcat ttattgtaaa aaaagctgat
      181 atcaaagaga atggttacac aagccaagtt tatatattaa acaataaaac tggcgaatta
      241 aaacaaataa cgtctataaa ctctgttgga gcatatgtct gggaagatga aaatactatc
      301 ttgttccctg ctttgcgaaa tgaaaaagta aaagaagctg tcaaaaacgg taagcaatgc
      361 atgagttact atgctttatc tttaaatggt ggagaagcag aagaactttt tagactccct
      421 atcaaaggtg gtaaattgaa tcctatagga aaaggtttgt atgcagttat tgattcttat
      481 gacaatgacc gacctattgt agagggattg ccagaagaag aacaacagaa aaaaataaat
      541 gcttataaca aaagacatta tgaacatttc aaagaaattc cttatgctgt caatggggag
      601 ggatatatta gcagaaaaag aaaacgccta tatatatacg acagtaatat aaataagttg
      661 aaggcaatta cagcacctat gtttaatgtt gtaggtatga aaataagtga tggaaaaatc
      721 ttatatatag ggcaagagtt taaagatgta aaaggtttga aaaatggggt ctatgtattt
      781 gatacaaaca caaacacaaa tatctgtata ttagaaaaag ataagtatat tataaaaggt
      841 tttgaattat atcaaaatca agtgatacta aacctaactg atgctttatc ttatggaaat
      901 ggagaaaatg gagattttta tactattgat attgatacga aagaaatgaa acttctttca
      961 gggcatcaac atcattgtat tggaaataca gtaacatctg atgttaaaat gggtgctggt
     1021 cagacaacaa aggtagatgg agaatatatt tattacactt ctacagtaga tatggactgc
     1081 attattgaaa gaatacatat tccaacagga aaacaggaaa aagtaacaca gacaggttca
     1141 gttgatttta tagatgtaaa agatggcaat attgtatgtg tgggttgttt aggaaatgga
     1201 ttaccagaag tttatacagt agagaatgga acattgtgta agaaaacaca tttaaatgac
     1261 catattttag aggaatataa aatttctgtg ccagaataca tagagtcaaa aggaagttct
     1321 aaatgggaaa tacaaggata cgtatataaa ccagtagatt atgaggtggg gaaaaaatat
     1381 ccagctgtat tggcgattca tggaggacca agattgacat atggaccata ttttatgcat
     1441 gagatacagg tatttacaag tgcaggttac tttgtattct tctgtaaccc aagggggtct
     1501 gaaggtcgtg gcaatgcttt tgcagatatt aggaaacagt ttggagatat tgattatata
     1561 gattttatgg aatttacaga tactgtactt gaaaaatata cagacattga taaaacaaaa
     1621 ttggctgttg aaggtggaag ttatggtgga tttatgacga attggataat tggtcataca
     1681 aaccgttttg ctgttgcttg tgctcagcgt tctattgcaa actggagtag catggagggt
     1741 acaacagaca ttggttatta tttctgcaaa ggacaaacag gtgcttctca tatggaaaac
     1801 cacgagcttc aatggaaaca gtcacctctt gcttatgctg ataaatgtgt tacaccgact
     1861 ttatttttac atggtgaaaa agactataga tgctatatgc aagaagcatt ccagatgttt
     1921 tcagcattga aaattcatgg gtgtcctaca aaactttgtc tatttgaagg ggaaaaccat
     1981 gaactgtctc gttctggtag accaaaacaa aagctacaaa gattagttga aatgctagat
     2041 tggttttctg tgtacataaa aaaagataac ataggacagt agaaaggaga atataaaaat
     2101 ggaaaaaata aagattgaaa gttcagatat ttttgaatat atattccctc atgatatttc
     2161 ttgttcacct gatggaaagc atattgctta tattatatca aatatcaacg aggaaaaaga
     2221 ctgctatgaa catgacttat atgtgatgga catcaaaaca gaaaaacaaa tacatatgac
     2281 tcaaacaaaa gatgtaacat catttagttg gatttctaat acagaactac tttttacatc
     2341 taaaagaaat aagccaaaag caggtacaac agatttttac actatttcta tagaaggagg
     2401 agaagcaaaa gaagcatttt ctattccaaa agcttgtagc gtacctgtat cacttggaaa
     2461 taaattgtgg ttattgacaa caaaaaatcc aacggattct aaaaaatcag aaccagatag
     2521 ggcagtagag ggtgtagatt attggacatt tacagataaa ccttttataa gagatagaga
     2581 aaatttttct caaagaagac gtgtaacatt agaattatat caagaaggtg aaaatataac
     2641 aaaagccatt acaccaaaat tttgtgaagt agcaggtata gacgtttctt ctgataaaaa
     2701 cagaatttta tatactggtc agatttatga agattgtgca actccttttt ctggactttg
     2761 ggagtatcat atagacagtg gtgaaaccaa agaacttgta ccacaaggaa agtaccaaat
     2821 aagtttggca aaatatattg gcaaagataa agtaatgttg caagcttcaa cattagaacg
     2881 ttctatcaca cagaatcatg atatttttat acttgatttg agtactagtg aaataaatat
     2941 gattgctagt ccagatggta tgtatgctac attactggat gtagatgcag tatatggtgg
     3001 tggacgtagt aataaagtta taggagataa atttattggt gcaagaattt gcagaactat
     3061 gacagaattt aatgagtttg atacaaagac aggtaatata cgtatcatca caaaagtaga
     3121 tgcttttaca tcttttgata tttacgacaa caccatgtac acagtaatgt tgaaagatta
     3181 tgagttagca gaaatatata gcattgatat gactacaggt acaatgaaaa aaatgactgc
     3241 tttttctaaa ccatatttag atactcataa ggtttcatta cctgaaaaat tgacgtttgt
     3301 agcaaaaaat aaagaagaag ttgatggttt tgttatacca cctatagatg ctaaagaagg
     3361 cgaaaaatat cctgctgtat tgttcattca tggtggacct aaatgggcat atggctatat
     3421 gtttacacac ttgaagcagt gtgtaacatc aaagggaatg tatgtgattt attgtaaccc
     3481 tcatggtggt gacggttatg gtgaaaaatt cttagaaatg gtagaaagat gggggtatgt
     3541 tgactatgag catcttatgg aatttgtaga tacttgtatt gaaaaatatc ctgggattga
     3601 tgcagaccgc ttaggggttg cagggggaag ttacggtggt tatatgacaa actggataat
     3661 tggccataca gaccgtttca aagcagcagt ttctcaaaga ggtattagta acttgattac
     3721 agcttcttta attattgact ttggggatag aattatgaag cagacttgtg gagataagac
     3781 gccttggaat catgaggagg ttctttggaa tcattcacct attaaatatg tgaaaaatgt
     3841 taagacacca acattgttct tacatagtga tagggattat agatgtttta tgggggatac
     3901 tttccaaatg tttacagcat taaaacagct tggtgtagat acagaaatgt atttgttcca
     3961 tggagataca catggattgt ctagaaatgg aagaccatct aatagaattg cacgagcaaa
     4021 tgctattgta gattggtttg aaagatattt ataataaggg ggttattcta tgagtgatat
     4081 gttattatgg tttagtgtcg tattagtttt atttgctatt ggggatttaa ttgcaagcaa
     4141 aacaaaagca aaagtatcag ctgtatttgt tacactactt ttatttttga ttctatttgt
     4201 cacaaaagca ataccagctg atattattga aaaagcaggt atgaccgcag cagctagttg
     4261 gagtgttcca atgattatgt ttagtatggg aacaatgttg aatgttaaac aatttataga
     4321 cgaatggaga acagtattga cagcatggct tggaattgta gcagttatag tctgtgtctc
     4381 attatgtatt cctttgtttg gaaaatcaac agtattgaca agcataccag ttataaatgg
     4441 agctttacct gctactacta ttatgacaca agcagcacta gaaaaaggac tgacacttgc
     4501 agcagctaca gcaacagttg tatttgcaat tcagaagttt attggtacac caatagcttc
     4561 aagagcagcg ttacaagagg caaaccgtct tttagtagaa tatcatgaag caaaatcaaa
     4621 aggcattgat ttagcaaatg ttgatactga taagaaagaa gcagagaata caggcacaaa
     4681 agtaaaacaa gctttctgtg aaaaatacga taaatattat tctacaaatg tttgtatatt
     4741 ttttatagca ctattctctt atctagggta tgaactttca gagattattc atgtaaatta
     4801 ttctattgta tgtcttgttg ttggtgttat tgtaacacgt attggtattg taccaaaaga
     4861 cattttggaa aaaggaaaaa tcaagggatt tattaacatg gttgtgtttg cagctgttat
     4921 tccttcttta gcaaaagttt ctttgacaga tttaattagt ctttttgtac caattgtggg
     4981 tatgtttgca gcttctatca ttggtatatt ccttatgatg aaggtgttac caggttggaa
     5041 aattatcggt tctaaacctc ttgcttttgg agtagggttt tgtcagatgt tgggattccc
     5101 aactacatat ttaatttcaa atgaagtgtg taatgctgtt ggagaaacgg aagaagaaag
     5161 agcatatttg atgtctaaga ttatgcctaa gctggtagtt ggtggtatgg cttgtatgat
     5221 ttctattgta gtagctggtc ttatggtacc aatgttgtag ataggtttca tagttaaaaa
     5281 gtggtgttaa tttataggag aagctaaaaa gtagtattaa tttataaaat aattttatat
     5341 tagttaattt gttataagaa tattttcttt ttatgattat aattttgatt ttataagaga
     5401 gcagattttt tatttgctat gctaatagtt ataatttgtt tttatttgct aaaatgcaac
     5461 tttaatttga tttatgtata atatagggta tttaataata tttttgaaga atttttataa
     5521 aaaattaaaa agaggagaaa aaagggagtg ccacaaagtg aactttttag ttctaaggca
     5581 ctcttttttg tacgaaatcg aatatatttt caacgtttta aaagtgagaa agagacttat
     5641 ctctagtttg ggatagccaa att
//
LOCUS       DACTGW010000216         5662 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.217, whole genome shotgun sequence.
ACCESSION   DACTGW010000216 DACTGW010000000
VERSION     DACTGW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.217"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..120
                     /locus_tag="I9573_003107"
     CDS             <1..120
                     /locus_tag="I9573_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT4797704.1"
                     /translation="DLSIREWICPSCNETHDRDINASINILKEGLRLITTQNK"
     gene            239..757
                     /locus_tag="I9573_003108"
                     /pseudo
     CDS             239..757
                     /locus_tag="I9573_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            784..2145
                     /gene="rlmD"
                     /locus_tag="I9573_003109"
     CDS             784..2145
                     /gene="rlmD"
                     /locus_tag="I9573_003109"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAT4797705.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            2668..3702
                     /locus_tag="I9573_003110"
     CDS             2668..3702
                     /locus_tag="I9573_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HAT4797706.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPYEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKNKKRKKI"
     gene            3789..3992
                     /locus_tag="I9573_003111"
     CDS             3789..3992
                     /locus_tag="I9573_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HAT4797707.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            4163..5323
                     /locus_tag="I9573_003112"
     CDS             4163..5323
                     /locus_tag="I9573_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HAT4797708.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVIIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
BASE COUNT         2246 a          560 c         1110 g         1746 t
ORIGIN      
        1 gacttaagta taagagaatg gatttgtcct agttgtaatg aaactcacga tagagatata
       61 aatgcaagta taaatatatt aaaagaagga ttaagactaa taacaactca aaataaataa
      121 cttatgtaag aaccgtagga actacgggga tagcctgtgg agaattagta agacatattt
      181 tagtatgcaa aattctgtga agcaggaacc ctgtgacttt agtcatggga ggttcaggat
      241 gaataaacct gatggatata tatcagcaac tacagataaa tatgacccaa cagtattaga
      301 tttaattgat ttatcatact tagcttttga accatttcct gttggaaggt tagataaaga
      361 cacagaggga ttattagttt taactaatga tggaaagctt tctcatagag ttttatctcc
      421 taagaagcat gtgcctaaga cttattatgc aaaaattgat ggagtagtta ctgaagaaga
      481 tgtagaagct tttttagaag gtgtagtgct tgatgacgga tataaaacta tgccatcaca
      541 gttaaatata ttaaaaagtg atgatgaatc agaaatagag cttacaatac atgaaggtaa
      601 gtttcaccaa gtaaaaagaa tgtttgaaag cgtaggcaaa aaagtagtat acttaaaaag
      661 attgtctatg ggcaatttaa aattggatga aagtttagag ttaggtgagt atagagagtt
      721 aacagatgaa gaagtaaaat tgattgaaga aagataataa agatactata gtaaattgga
      781 ggaatgtctg tgaagaaaaa agatgttata gaatttgaag tagataaaat ggaatttggt
      841 ggtacatctt taagtcaagt tggagataga gttatccata tgaagggtgg aataagtggt
      901 caaaaggtga gagctggagt taaaaaagtt agaagtaaga aagctgaagt taagatgata
      961 gaattattag agaactcacc acttgaaact gaagaaactt gcaagcactt tagagagtgt
     1021 ggaggatgta cactattgtc tgtgccatat gaaaaacaat tagaaataaa agaaaaacaa
     1081 gttatggatt tatttttaaa gcaagattta tttggatttc agtttttagg gatagaagga
     1141 agtccagaaa acaaatatta tagaaataag atggaatata cttttggaga tgaagtaaaa
     1201 aatggacctt tgacattagg tcttcataaa aaaggaaagc atatagatat acaaactgtt
     1261 gaggagtgta tgttggttga tgaagacttc tcaaagatat tagtttctag tgtagagttt
     1321 ttcaatgaaa aaaagcttcc ttattataga acaatgaatc acaaaggata tttgagacat
     1381 cttgtagtta gaaaagggat tcatactaat gagattatgg ttaatatagt aacttcttct
     1441 caagaagatt ttgatatgaa tgagtttaaa gatatgcttt tagggattga tttaaaggct
     1501 gagttagtag gtgtacttca tactattaat gatggtttag cagatgcagt ccaatgtgat
     1561 gagttaagag tattgtatgg aagagattat atacaagaag aaattttagg tcttaagttt
     1621 aagatttcac cattttcgtt tttccaaacc aatactaagg gagctgaggt tctttatagt
     1681 attgctaggg attttattgg agattacaat gataaggttg tatttgactt gtatagtggt
     1741 actgggtcta ttggacaggt tatggctggt gctgctaaga aagtgtatgg tattgagata
     1801 gttgaggaag ctgttgttgc tgctaatgag aatgctaagt tgaatggact tactaattgt
     1861 gagtttattg ctggggatgt ggctaaggtt gttaaggatt taaaggacaa gcctgatttg
     1921 attattgttg accctcctag accaggcatt cataaggatg ctattaggga tatttgtgga
     1981 tttggtgcta aggagattgt ttatatttct tgtaatccta agtctttggt tgttgatttg
     2041 gttgatttta aggggtatgg ttatgagatt aagatggtta agtgtatgga tatgttccca
     2101 aatacgccac attgtgagac tgtagttaaa cttattagag aataacaggg tttcagactt
     2161 ttatattgtg tcttaaaagt agatttgcca cctatttgcc accataatta tttttgggtg
     2221 gcaaatttat tctacaatga tttcaaaaat atttaccatc tggtttctca tgttattagt
     2281 tacatgtaaa tatgtatcca tagttgttga agttttagca tgtccgagta tatgctggat
     2341 atcttttata ttagtaccat tttctaataa catagtatca gggtttttaa aataaagtgt
     2401 gtattcttct agagtagtga taattaaaat atcaaatatc actatacaaa ggaaagtgca
     2461 ccctttttat gtttagcaaa aacgaaatat taaaaaaact tattgaagaa tatgatgtca
     2521 aaacaactac agatattcaa gatatgctaa aagatttatt tacaaataca attcatcaaa
     2581 tatttccatt gtcgaatttt aacggtttaa acataatata ttgactgata taataaaatc
     2641 atagaatatt ttagggggaa atagattatg tttaagaaaa agttaatagc tgttgcaatg
     2701 agtataacta tgattagtgt agggagcttt tcgtatgctc attcaggaag aactgattca
     2761 tcaggaggtc atcgtgataa taaaaataaa tctggattag gaagttatca ctatcattgt
     2821 ggtggttatc cagcccattt acataataat ggtggttgtc cttatactgg aggaggaagc
     2881 agttctggaa caacaacttc ggttaataat gaagaaaagc aaaagaggtc tgttggagaa
     2941 aaaggatata atcaagggta tgaagatggg tataaaggaa attatagttc atcaaattat
     3001 agtggagatt atagtgatac atatgaatca aagtactcag aaggatatga aaaaggtaag
     3061 gctaaattag aagaggaaga aaaggtggct aaagagactg gatataattt agggcttaca
     3121 ggtgctaaat ctaataatac atatgaaaaa gaagttttaa aaaatgctta tgataccgga
     3181 tattcaactg gatacaatga atataagact aaaaagatag aagaatataa agctaaaggg
     3241 atagaggata gcaataaaga taaagaaaaa atgacatttg aagaaaacat agattctgaa
     3301 tttatagatg cttataataa tgcttatgac gaaatacaag aacaattaaa aaatgattac
     3361 actactcaag gatttgagag tgctatcaaa ggagaaagat ttgacacatc aactataggt
     3421 aatgttaaat atgctaattg gtttaaagaa ggatatgata atggtaaggt taagttacct
     3481 aaagcaaaag aaagtgtata taatcaaggt tacaatgaag aagattttag tataccatat
     3541 gaaatgaagt ctattgaaac taagttaaaa ggtgtatatg atgaaggttt agaaaaaaga
     3601 gaagaagaga aaagtagaaa tgctacttat ggggttggag ctggaacagc agctgtagtt
     3661 gctggagtag tatataaaaa taaaaaacgt aaaaagatat agatgctaga tataccaatg
     3721 atgacaagtg tataacaaat ataattttaa gtataaatat ttaaaatata gaagggaagg
     3781 ttatatctat gggatttaac tttaggaaaa gcataaatct aggaggagga ctcaaattaa
     3841 atttaggtaa gaagagtgta ggtataagtg ctggtgttaa aggtgctaga gtgtcagtta
     3901 attcaaaagg aagaaaatca gctacattga gtatacctgg aactggaata tcatatacaa
     3961 aaactagtac aaagaaacgt aaacctaaat aaatatttga tatactaaga aatagactgt
     4021 tacaaatagt ctatttttta gtatatagat ttaaaaagtg acaaaatttt gaaatataaa
     4081 ctaatttcat aaaggaaaat attcttttat gtagaattat aatatttaag taatattata
     4141 aaatttatag gaggatttca ttatgaaatt aagcaaaaca ctattggcaa tagggatgtc
     4201 tgtttcatta ttgattgcta gttgtccaac atcaaacgct cttagttcta ttaattcaat
     4261 aaaaggtaca aataagtatg aaacagcagg tttaatagca gataaacaaa attatgatat
     4321 tgcaatactt attaattctg aaaatggatt atctgatgga ttgtcagcaa gtggattgtc
     4381 aggagctaaa aatgccccaa tattgttaac taagaaagat acaatccctt cagaaacatt
     4441 aaaaagatta gaaaaggtaa aagaagtata tctaattgga ggaaaaaatt cgataggagc
     4501 atcagttcag aaaatattaa gtgataaaaa aataagcata aatagaataa gtggaactga
     4561 tagaatagat acaagtcata aagttgcaaa agaaatagga agagtaagta cggttaaaaa
     4621 agtaatatta actaatgctt ttaaaggcga accagatgct atgagtgttg cttctatagc
     4681 agttagagat aaatctccaa tagtattaac tgatggaaat actacaagtt ttgctacaga
     4741 tggattagaa acttatgcaa ttggtggaaa agtcagtatg agtgataagt taataaatga
     4801 aactaaagca acaagaatag gtggagtaga tagatacgat acaaataaac aaataataaa
     4861 taaattttat aatggctcaa aagaatttta catagcaagt gggactgatt tagtttattc
     4921 tttagttggt tctacaattg cacaaaaaac acctatagtg ttagttggaa atggaagtaa
     4981 taagagtata ttaaaaaatg caagtaaaat aactgctatt ggaaatattg atgatgttat
     5041 tataaaacaa tgtttaaata taactaataa tgttggggat tcaaatacag gtattgtaaa
     5101 agatactaat tcaaataata caaacaaacc aagtaatgag cctagctcta ataatcctgg
     5161 attaactgat aaaagtacag tatatgtaac tcctaatgga aaatcatatc atctaacaaa
     5221 aaattgttct acactaaaaa gaagtaaaac aataaataaa gtaacattaa aacaagctaa
     5281 gtcacaaggt aaaatagacc cttgtaatgt ttgtgtaaaa taatgtatta gccaaatata
     5341 tattaaaaaa gacaattaat taagattgtc ttttttattg aagaaatata aatttatatt
     5401 atgtattatt aaaaagaaaa ttattaatta taaaaaataa taataaagac gataagtatt
     5461 aaaattttat gtttccaata tttatgatat aattaatatg gcaaggaata aatatttgaa
     5521 aaagctatga gtggtgtttc catggaagtt tcttcacttt cttatgagag gaggtgaaaa
     5581 ctatggaaaa tctaatgatg agtattataa ctggtgttat agccagttac atttacgata
     5641 aattaaagag ccactctgac ca
//
LOCUS       DACTGW010000217         5660 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.218, whole genome shotgun sequence.
ACCESSION   DACTGW010000217 DACTGW010000000
VERSION     DACTGW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.218"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            255..1016
                     /locus_tag="I9573_003113"
     CDS             255..1016
                     /locus_tag="I9573_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797709.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            1019..1837
                     /locus_tag="I9573_003114"
     CDS             1019..1837
                     /locus_tag="I9573_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797710.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            2017..2619
                     /locus_tag="I9573_003115"
     CDS             2017..2619
                     /locus_tag="I9573_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HAT4797711.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     gene            3482..5401
                     /gene="thrS"
                     /locus_tag="I9573_003116"
     CDS             3482..5401
                     /gene="thrS"
                     /locus_tag="I9573_003116"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAT4797712.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
BASE COUNT         2295 a          533 c         1014 g         1818 t
ORIGIN      
        1 tgagtttaga ttagcaataa aaagatatat ttattaaatt tgtgctaact gtacggaata
       61 tactgcttaa gtttagatta gtgataaaaa gatatattta ttaaatttga accaactgta
      121 caaagctata ctaattaagc ttagattaac tatataaaat tataacaata agtgtgtggg
      181 cagaaaaatt ctgctacaca ctttatttat attcatataa aaaaattatc aaaaaaaaga
      241 aaaaggagta aaaaatggaa tttacaagag aaccatttat actagatgac gatttgtgtc
      301 tagctttggt ggacaaaaga attagttatc taatggaaaa tgaattaaaa ttaagaaata
      361 tagagataat aaaaacaata gaatgtaaag aattatatga aagcataaaa tatcacccgg
      421 atatatgtat ttgtaattta ggaaaaggag atataattgt tgcgccgaat gtatataatc
      481 aatacaaaga gttattaagt agatataaat ttaatataat taagggagaa gctaatttat
      541 taaatagata cccttatgat ataccttaca atattgcgat agttggtgac tatgctatac
      601 acaattttaa gtatactgac aaagcaatat tagattatat tgaaaataat aaacttaaaa
      661 agataaatat agagcagggc tattctaaat gctcaatctg tatagtagat agaacgtcta
      721 taataacatc agataaaggt atttggaaaa gtatgaagaa tacagaaata gagtgtttat
      781 tgatagaaaa aggtcacata aatttatttg agatgaatta tggatttata ggtggatgta
      841 cagggttgat ttctaaggat aaactagctt tttgtggaga tgttaaaaaa catcctgact
      901 atgaacgtat aaaaagtttt gtagaaagta aaaacaagga gatagttaca cttagctgtg
      961 aaaatttact agatataggt tctatagttc ctctcatgac aagaaaggag cgataacttt
     1021 ggttgagaag gataatattt cttccatgaa atttttgagg aataataatg taaacgatag
     1081 attaatatat acgaatgaca ttgtagaagt agtgatagat tctgcaaagc aagaagtgtt
     1141 aaataaaaaa atacttaatg gtactaaagc tgaacaaaga gctttaaata ttgatgagat
     1201 atcaacggaa ataactagta tgataataag tataatgaaa gagaaacttt tgaaagaata
     1261 cattctaaaa tgctatgggc gtacatatcc tgaagataaa aataatatat acatatattc
     1321 actgaacata tttgctaaaa aagagatttt tatgagagaa acagtattta ctagggtgag
     1381 aaattatata gaagaaaatg attttataaa tgtagaaggt tttattagat ttagaatgag
     1441 agaatttatg aaatatattt ctgctatagg tgatattgca gttgaggagt atttaataaa
     1501 gaaagaccaa gatgaattta taagagtttt aaaatacttt atagatactc aagaagaaaa
     1561 gattgatttg cttaaagttc atataatgga agataataca tttgttttat acgataaaaa
     1621 tggaaataag atagacagta tagatgatga agaaattata aatatggtaa taagagaaaa
     1681 tttaaattat gaggactttt taataagtac cttattaact ctatgtccca aaaaaataga
     1741 aatattggat ttgttaaata ataattgctc aaaggaaatt atagatactg tagaagcaat
     1801 ttttgaaaat aaagttagta taattatgga aaactagaat ctaagcatat aaaagctagc
     1861 catataaaat taaaaaaatt ctttttaaat cgacacaaaa gacttataat tgtatataaa
     1921 gctatagtat aactagagtt attaagtata aagaatatct acttgattaa tgcaatatag
     1981 ctggcaaata tacaattgat aaggagtaat tttataatgg ataatttaat taggatactg
     2041 atattagctg ttattggtgg atttataggt tatgttacga atgtagttgc tataagactg
     2101 atatttagac caatagagcc tattaaaata cctattttaa atatagaaat agtaggtctt
     2161 ataccaaaga gaagagcaga aatagcagca aatgtaggag aaataattca agaagaattt
     2221 ctttctatgg acgaaatact tgcaaatatc attactgacg aagataagga agaagttgta
     2281 agatacataa aagcaagggt aaaaattata atacatgaga aagtttcatt tatcccgagt
     2341 ggtataaaaa acatgataca agattatcta ggagagataa ttgagtctga agttaagcaa
     2401 agtatagatg aattgagtaa aaatataata aataaagcaa atgaacgtat agatatacaa
     2461 aaaatggtcg aagataagat taatgaacta gatttatatg aattggaaga gataataata
     2521 agaatagcta agaaagaatt aaaacatata gagtttttgg ggcttgtctt agggttttta
     2581 ataggtatag ctcaagggtt aattactatg tttatttaaa tatatgagaa gtagcattta
     2641 tttaagtact aattatattt acctatttga gtgaattact tattaatagt atttagatat
     2701 taattgtatt tacctatttg agtgaattac ttattaatag tatttagata ttaattgtat
     2761 ttacctattt gagtgaatta cttattaata gcatttagat attaattgta tttatttatt
     2821 tgattgtgtt acttattaat agtatttaag tatttactgt actacttatt aactatatta
     2881 agtattgatt gtatttaagt atacaaaacc taaaaagagt acaaaaatta gaagttagtt
     2941 ttaataaaaa ttagttttag tatttatcta gtcatatcta tcatgcatgt ggatacgttt
     3001 taaaaatttt aaatttatga aaatattata atatcactat aaatcaagag attaatttaa
     3061 tataaaaata taagaatatt ggtttacagc tgattgagat gaaaaataat gggtaattaa
     3121 tatattacat taatgttgat tgatgtataa ctatctgtta taatgaaaat atattaaaaa
     3181 tcatagtgtt gaagaggaag agtagagtag aaaccattct cagagaattt gggtctatgg
     3241 ctgagagccc atgtaatagg tctatttgaa aactaccttg gagctgagct tattagcagt
     3301 atttgtattt gtactatacg tgaaagtgct cgggtgacga ccttatagtc atcaagaggg
     3361 ttgtatatac ttgtatacgc caacttgggt ggaaccgcga attaatatcg tcccaaacat
     3421 atttggggcg attttttatt acaaaaaatc taaaaattat ttcaataagg aggaaaaaac
     3481 aatgattaaa gttgctttaa aagatggctc aataaaagaa tttgaaaatg ctatttctgt
     3541 tatggatgta gctaaatcca ttagtgaagg attagctaga aatgtggtag cagcgtctgt
     3601 gaacggagag gttgtaggtc tggaccacat tatagatact gattgtgact tgaatttatt
     3661 taagtttgaa gataaagaag gtaaggaagt ttttagacat acatcagcac atatattagc
     3721 tcaagctata aaaagacttt acccagaagc aaagcttgct ataggaccaa gtatagaaaa
     3781 tggattctac tatgatatag atttagacca tagattagtt ccagaggacc tagagaaaat
     3841 agaagctgaa atgaaaaaaa tagctaaaga agatttaaaa atagaaagat ttgaacttcc
     3901 tagaaatgaa gctttagagc ttatgaaaga acaaggagaa gactataagg tagaacttat
     3961 atctgattta cctgaaagtg aaataatatc tttctacaag caaggtgatt ttactgattt
     4021 atgtagagga ccacatttac catctactaa aaaggtaaaa gctgttaaat tacaaagtgt
     4081 agcaggtgca tattggcgtg gtgacgaaaa taataagatg cttcaaagaa tatatggaac
     4141 ttcttttgag aaaaacaaag acttagaaga gtacttacat ttactagaag aagctaaaaa
     4201 gagagaccac agaaaattag gtaaagaatt aggattattt atgatacctg aagaaggtcc
     4261 aggattccct atgtttttac caaaaggtat ggaacttaaa aatgaattat taaaattctg
     4321 gagagaaata cacagaaaag ctggatatat tgagatagaa tctccaatca tattaaatag
     4381 aaaattatgg gagacttcag gacactggta tcattacaaa gaaaatatgt atacagttaa
     4441 aatagatgat gaggattatg ctataaaacc tatgaactgt cctggtggat taatttacta
     4501 taactctcaa ctacattcat atagagattt cccaatgaga gttgcagagc ttggtagagt
     4561 tcatagacat gagttatctg gtgcattaca aggattaatg agagttagag catttactca
     4621 agacgattca catatattca tgttaccaga gcaaattaag gacgaaataa aaggagtagc
     4681 taacttaata gatggtatct acaaaacttt tggatttgaa tataatttag aattatctac
     4741 aagaccagag aactctatgg gaagcgacga agagtgggaa gcagcagaaa atggattaag
     4801 agaagcttta gaagagttag gtctaccata tactattaat gagggtgatg gagcattcta
     4861 tggtcctaag atagacttcc atctaaaaga ttgtttagga agaacttggc aatgtggtac
     4921 tatccaatta gatatgcaac ttccaagaca atttgataat acttatattg gtcaagatgg
     4981 tgaaaagcat agaccagtta tgattcatag ggttgctttt ggtagtatag aaagatttat
     5041 aggtatatta atagagcatt atgctggtaa attcccagtt tggttatctc caactcaagt
     5101 taagatactt cctatatctg ataaatttat ggattatgca aatgaagtta agaaagagtt
     5161 atttgataag ggtataagag ttgaattaga tgatagagct gaaaaaattg gatttaagat
     5221 aagagaagct cagcttgaaa aagtgccata catgttaata gtaggtgaaa aagaagtagc
     5281 tgataataat gtttctgtta gatcaagaga taaaggtgaa ataggtagta ttaaattaga
     5341 tgaatttata gctagtattt caaaagaaat tgaatcaaga gaaagtatta ttcaagacta
     5401 atatataata tattagtagc taagatattg ttatataaga taagtttaat caatatgttg
     5461 atatgttgat acatagtgat taaatttatg aaaagatgag tataattaga ggtacagtta
     5521 atcatatttt tatgattaat gtacctctag ttttttgtat ggtagattta ttaataaata
     5581 tatcatagaa gtgttttgaa catggaaata tttatgtgca ttctttaaat aaaagtttat
     5641 tttttgaatg ataattaagt
//
LOCUS       DACTGW010000218         5652 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.219, whole genome shotgun sequence.
ACCESSION   DACTGW010000218 DACTGW010000000
VERSION     DACTGW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.219"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            112..741
                     /locus_tag="I9573_003117"
     CDS             112..741
                     /locus_tag="I9573_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4797713.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(855..2369)
                     /gene="glpK"
                     /locus_tag="I9573_003118"
     CDS             complement(855..2369)
                     /gene="glpK"
                     /locus_tag="I9573_003118"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAT4797714.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2612..3154
                     /locus_tag="I9573_003119"
     CDS             2612..3154
                     /locus_tag="I9573_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT4797715.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3168..>5652
                     /locus_tag="I9573_003120"
     CDS             3168..>5652
                     /locus_tag="I9573_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4797716.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSINQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENK"
BASE COUNT         2103 a          870 c          776 g         1903 t
ORIGIN      
        1 aacatgcaca atatttgtac atattgtaga catataaacc tataaattat acaatagtat
       61 tgttaagatt atatattttt ttaacataaa ttttgtattg ggggaaaaac aatggataaa
      121 aactatacga tgccaatata ccaaaaaata gcgttggata ttgcaaataa aatatacacg
      181 ggtgaaatac aagaggattc agtactattt ggacgctctg ttttggctgg taaatacaat
      241 gtatctccag aaacgataag aagagctgtc aaaattttag aggacattgg cgttgttaaa
      301 agcataaagg gtaaaggtgt tattgtacta tctccagaca aagcttcttc ttttataaaa
      361 aaatatagag atattacaaa tatatcatca tataaatcaa cactttataa tttaatagat
      421 actaaatcta atttagaaaa tgaaatatta gatactataa ataaaatttt agactattcc
      481 aacagacttg aaataatcaa tcctttagtg cccgttcagt tcaccataaa ttctaattgc
      541 aaatatattg gtcaaacagc tgcacaaact aaattttggc aaaatacagg tgctacaata
      601 gttgcaatta aaagaggtga agaacttata atatctcctg gaccatatat tgaattttta
      661 gaaggagaca ttctcttagt agttggtgac caacacattt acaattctat accaatgttt
      721 ttatatgaaa acgaaaaata aattatataa tttagtatga ttatataaaa atgcaaaatt
      781 aacaacaaaa aaagagcttc atattttaca taaagctctt tttaaattca aataaattta
      841 actttgatta atttttaaat atccaattca tcttcttttt cccagcttaa agctctcgat
      901 acagcttttt tccatccttt atataattta catctcttct cttcactcat atttggatta
      961 aactcttttt ctactgacca ctttgaagta atttcatttc ttccaatata gaatccaaca
     1021 gacaaccctg ctaaataagc agctcctaaa gctgtagttt ctactatttt gggtctatct
     1081 atatctacat ttaatatatc tgattgaaat tgcattaaaa aattattatt acttgctcca
     1141 ccatcaactt ttaatgattt tagttttaat tttgaatcgt tttgcattgc ttctaaaaca
     1201 tcctttgtct gataagcaat tgattccaat gtggctctta ctatatgttc tttttttgct
     1261 cctcttgtaa gtcctaaaat tgaaccccta gcatacatat cccaatatgg agctccaaga
     1321 cctgtaaatg ctggcactac atatacacca ttagtatcct ctaccctatt tgcatacatt
     1381 tcactatctt ttgcagattc aattatcttt aattcatctc taagccattg aacagatgct
     1441 cctccaataa atatacttcc ttctaatgca tatgttactt ttccatcaac tccccaagca
     1501 attgttgtaa gtaacccatg cttagattta actatgttac tgcctgtatt cataagtaag
     1561 aaacaacccg ttccataagt attttttgca gtaccttctt caacacaatt ttgaccaaat
     1621 aatgctgctt gttggtctcc agcacatcct gcaataggta ttttagctcc tgataacatg
     1681 cgctcatctg tatgaccata aacatagcta gaaggtttta ctaatggcaa catgcttatt
     1741 ggtatgtcca attctcgtaa tatatcctca tcccattcta aatcatttat attgtacatc
     1801 attgttctag atgcatttga gtaatctgtt acatgtactt tcccacgagt taaattccat
     1861 attaaccatg tatctatagt tccaaataat aactctcctt gttctgcttt ttccctagct
     1921 ccttctacat tatctaatat ccattttact tttgttgctg aaaagtatgc atctattaaa
     1981 agacctgttt tttcttttat tgattcttcc aatcctttat ttttaagttc atcacatatt
     2041 tctgatgttc tcctacactg ccatactatt gcgttataaa taggtttacc agtataccta
     2101 ctccaaacaa ccgtagtctc tctttgatta gttatcccta ttgaagcaat ttcttctggt
     2161 cttatacctg ctgtttctat aacttctctc attacaccac tttgtgaccc ccatatttcc
     2221 atagggtcat gctctaccca tccagctttt ggataaattt gattgaactc tttttgtgca
     2281 attttcacta tttcgccttt tttattaaat agtatagctc ttgaactggt tgttccttgg
     2341 tctaatgcca ttacatactt cttttccatc accatattct ccttatcata taatatttaa
     2401 tttattaaaa agaaaaattt aatatcacat atttcaataa ataatcatac ttaagtacaa
     2461 atttaatata atagatattt ataaaaagac acttattttc tatcatatct atataataat
     2521 attatacatt atagcttaca tattgattaa taaattttaa aggagtataa taaacagtgt
     2581 tcataataat tttaataaaa gaggtggtaa tatgccaaag attttagaaa atgtaaaaga
     2641 agatatatta aaagtatctc gggatatgat tttagaagaa gattactcga atattagcat
     2701 aaggaaaata gctcaaaggt gtggtataag tgcaggtaca gtatataatt actttaattc
     2761 aaaacaagaa ataatagaat atatcacaaa atctgaatgg gatttattga taaggcgaat
     2821 tgaatactcc aataaaaaca ctgaagatta cataaaaaaa ctaaacatta tttttactga
     2881 aataagaaat tttataaaca aagttcataa tatccaatac aatgactttt taaacacttt
     2941 tgaaacagag agattttttg aaatgaaaaa acacaaagac gactttcatg aacagctttc
     3001 aaataaagta tatgaagctt tagaaaaaga aagttttttt aataatgaca aattagtatg
     3061 caatatcata ataaaaatgt ttttttcata ttcaacatat cttcatatag aatttgaaga
     3121 tttaaaaccc tatattgaaa aattaataca ttaaggtggt gtttaccttg aatcataata
     3181 ctagtattaa gaacaaggac agtaatccaa aaatattttt agtacttaca ctttatctat
     3241 taggtatatt tatgggagct attgatactg gaatagtatc accagcaaga acgattatac
     3301 aaaatagcct tggagtaaat gaaaaaacgg gaatttggat gattaccata tacacattaa
     3361 catacgcttc tgtaattcct atctctggaa aattagcaga taaaatagga agaaaatatg
     3421 tgtatctagt tagtatattt ctatttggta gtggttcatt aatatgtgga ttatcatcac
     3481 tattttccaa tttctatatt ttattaattg gtcgtgttat tcaagcaatt ggtggtggtg
     3541 gaatcatgcc aattgctaca gcagaatttg gaactacctt cccagaaaat aaaagaggca
     3601 tggctttagg tttagttggg gcaacatatg gtattgccaa tattttaggt tcaagcattg
     3661 gtagcacaat tctaagtatc tttggaactc aaaattggaa gtggctattt tttgtaaacc
     3721 ttccaatttg tttaatcata cttattggtg gagttttctg tattaaaaac aataagtctg
     3781 aatctaaaga aaaaatagat aagcttggta cattgatgtt gatatgtata atcgtatcac
     3841 tattatatgg tcttatgaat atagactttt ttaattttaa aaattctata caagatatat
     3901 cagtttatcc atacttattg acatttattg tacttatacc tattttcata tttattgaaa
     3961 atagagctaa agaccctatt ttaagctttg aatatttttt aaatccgaga atattgatag
     4021 tacttatttt atctttaatt gttggtattg gaatgatggg tatggtattc gttccacagt
     4081 atgctgaaaa tgcattaaag ataaatgctg gttctggtgg atattttgta gctatattgg
     4141 gattatttgc aggtattgct gctcctttaa gcggaagact tattgacaag tttggggcaa
     4201 agaaaatact attactagga ttttctgtat ctatgatagg ctctctttac ctagttttaa
     4261 ttgctctaaa aaccaatact gtatttagtg tatgtgtaag tttaatattt atgggattag
     4321 gaatgggctt tactatggga actcctctaa actatatgat gttatcaaat acaagattag
     4381 aagaatctaa ttcagcactt gcaacattat ccttgatacg ctcaattggt acttcaattt
     4441 ctccagctat aatgattgga tttatagcac atgcaggatt atctgtgcaa gataatatta
     4501 tggacttagt tggaaagccc tcaactccta aaatagttca acttgaagaa cttgaaaaca
     4561 tgatagatga cttaaaatca gaccctgaaa tggcaaaaaa tttaaaaaat gtctctatcc
     4621 caaatatgaa tgatacttca aatataaata tggacatgac aggtggcaaa cttccaaaat
     4681 ccttacttaa taaagttcag tcagcagata caaccaacat taccaatata acaaaagaaa
     4741 tatcaacaac aatgtttgat gaaaaagttc catctataat tactaaaata cagaaaaatg
     4801 tacaaaaagg aatagatgga acacaaagtg gaataaacgg tattgaagat ggtgaaaata
     4861 aactaaatag tggtattaaa ggagttcaaa ctggtattga caatatggta aaagctagag
     4921 ctggtattaa tcaaggaata gatggtgtaa aaaaaggtat tgctggtgtt gacaaaggta
     4981 taaaaggtat ggaacaaggt cttgaaaaac aagatgaggc catcaaggaa cttacaactg
     5041 cttataataa aatacctact acagaaaaga ctcctactga taacgaaaat aactcaaatg
     5101 aacaaaacca gagtaatacc agtaattctg aatcagacga cactaagaat gattctacag
     5161 aaaataagaa taacaattcc agcgagaata aaccaaatgt aaatgaacaa aaagaaagtt
     5221 taaatgctca aatacagcaa ctaaaaaaga caagagatga cttaaataca aagttacaaa
     5281 aaaataaaac tcaaaagaac gaattaagta aaaaactaag atcaatggaa tctcaaaaga
     5341 aagagctaca aagcaagtta aataattcta taaaccaaaa gaaagacatg gaaaaaagcc
     5401 ttgatacaat gcaacagcaa aaaaaatcac tccaagcagt tttagcaaag acacaagagg
     5461 taaaaactga aataccaaag gcttttgata aatcaaaatt ggattatata aattccatag
     5521 aaaataatag aacaaaaata gaaaatacac tacaatctac tctaaactca ggattcaaac
     5581 agatgtatat aacagtattc tgtgtaaatc tactagcatt tataatatta ttgttctata
     5641 aagaaaataa aa
//
LOCUS       DACTGW010000219         5622 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.220, whole genome shotgun sequence.
ACCESSION   DACTGW010000219 DACTGW010000000
VERSION     DACTGW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.220"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            183..1454
                     /gene="serS"
                     /locus_tag="I9573_003121"
     CDS             183..1454
                     /gene="serS"
                     /locus_tag="I9573_003121"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAT4797717.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKHKLNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            1733..3238
                     /locus_tag="I9573_003122"
     CDS             1733..3238
                     /locus_tag="I9573_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAT4797718.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3352..4050
                     /locus_tag="I9573_003123"
     CDS             3352..4050
                     /locus_tag="I9573_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAT4797719.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            4335..5423
                     /locus_tag="I9573_003124"
     CDS             4335..5423
                     /locus_tag="I9573_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797720.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT         2271 a          560 c          930 g         1861 t
ORIGIN      
        1 aatttcattt atttaattaa atataatttg acaatggaca aaaaagtgag taaaatataa
       61 ataaaataaa aataacaggt tcggataaag aaaaaggttt cttaacctca agcataaagt
      121 atattatcta agttatttga gattaagaga taaatccaat cttaggtact gaggaggaaa
      181 aaatgcttga tataaaaaga ataagagaaa atttagattg tataaaaaaa gcaatggaaa
      241 gaaggggcga aaaagacttt aaccttgatg aagtagttaa gttagatgat gaaagaagaa
      301 agattcttca agaagttgaa gttatgaaac ataaattaaa tacagcatct aaaaatatac
      361 caaatcttat aaaagaaggt aaagatgtag aaaatgaaaa gattaaacta aaagagttat
      421 ctgataaaat taaggtaata gaccaaaatc ttaaagaagt agaagataag atggaatacc
      481 tattaatgag aataccaaat gtaccacatc cagaagtacc acaaggtgaa actgatgaag
      541 ataatgttga ggtaagaact tggggaaaga ctactacctt tgattttgaa tctaaggctc
      601 attgggaaat tggaacagaa ttaggtatac ttgattttga aacagcgagt aaaataactg
      661 gttcaagatt cactctttac aaaggattgg gtgctagatt agagagagca ttattaaatt
      721 tttacttaga cacgaataca aaagtaaatg gatatacaga agtcattcca ccatttatgg
      781 caaatagaaa tagtttttta ggtacaggac agttacctaa gtttgaagaa gatatgttta
      841 aaatagaagg tctggactat tttatgattc caacatcaga agttccttta actaatatac
      901 atgctaatga aatacttaaa tttgaacaat taccaataaa ttatacagct tatactccat
      961 gttttaggtc agaagctggt tctgctggaa gagatacaag aggtttagta agacagcatc
     1021 agtttaacaa agtagaaatg gttaaaatag ttgcaccaga agaatcttac aatgagttag
     1081 aaaagcttac taacaatgcg gaaactatgc tacaattatt gaatctgcct tatagagtag
     1141 taaagatatg tacaggtgat ttaggattta cggcttcttt taaatatgat gtagaagttt
     1201 ggatgccaag ttacaatagg tatgttgaga tttcatcttg ttcaaactgc gaagatttcc
     1261 aagctagaag agcaggaata agatttaaaa gagataaaga ttctaaagct gaatatgtac
     1321 acacattaaa tggctctggg ttagctattg gaagaagtgt tgcagctata ttagaaaatt
     1381 atcaacaaga agatggttct gtggttgttc ctgaagtttt aagaccatat atgggagttt
     1441 ctgttattaa ataagagtta aattatgtat ttttaagcaa tacaataata tagaagtaat
     1501 aaaaaataat taatataaaa gtgatacaat aatatagaag taataaaaaa atagttaata
     1561 taaaagtgat acaataataa tagaagtaat aaaaaaaaat aattaatata aaaatgtgta
     1621 caaactaatg aatattgata tagtaagcta tcatatgaaa gagtatgtac acattttttt
     1681 tactaatttt tcacaatatg aacataaata atttacagga aaggaatctt ttatgaaaat
     1741 tcaaaatact gagtggggat atattgaatg gaaacatact tatgatgaaa ataatccaaa
     1801 acaagctatg aatatataca ttgcagtaac tatgcctggt aagaaacatt ttaatcatgt
     1861 tcattatggt caagagcaga tgatatatat tttagaggga gagggtttgt atattataaa
     1921 tggagtttgg aagccatttt atcaaggtat gattttttat atagaatctg gtagcacaca
     1981 tgaaactata aatacaggcg atagagaaat aaaagaactt attgtttcaa ataatgtaga
     2041 tgatgtagga gagagtgaag ttatagatat taatcctaat aattatttaa agaagacctt
     2101 gataaattat agtgaaagta cattaaattt gtatgcagca gtagaatcaa ttcgtggtca
     2161 atttatagac ccgtttaaaa ttccattaat tatatatgat gattcatgga atatagtttt
     2221 aaaaaaccct tattttccac ttttttgttt tgaaaaatgt aatcctatga aatttcctca
     2281 aaattgtgat tgtatgaatc aaaaaagtag caatcaattt gtatgtgaat atgggataac
     2341 tatatataat ataccaatac tgtacaaaag caatagcata ggtgttataa gaggagggta
     2401 tgtactatta tctgatttaa atttagatac agagcataat aatttatatg atattccaga
     2461 gggagctgct agaagtataa aacgcctctt gaaacaaata tctaaaaata taataaattt
     2521 ttgctctttt aatgatataa gaaaggattt acaagaaaaa gagaagacta tagcaagaac
     2581 atatcattat ggagagcaac ttgaaatgaa tttaaaagta gctcaagata tggttactaa
     2641 tttaagaata aatcatcact ttttatttaa tacattaaat tcaatggcaa gcatagcact
     2701 agatgatggt agttatgatt tgtatagtgc aataatagat ttatcaagaa tgtttagata
     2761 tacaatgagg tctgatttaa gatttgtaga attagaatct gaaatattat acattaaaaa
     2821 ttatttgaat ttacagaaat tacgttatgg agatgcactt aaagtaaaat atcttattcc
     2881 agagaagtta tataaccttt ctgtgccttt taactttatt caacctatcg tagagaatgc
     2941 atttacacat ggatttagag atattgacac agaaaagcga attgaaataa ttgcaagatt
     3001 agattctcag tatgctatta tagagattca taataatgga actatcttag atggaaataa
     3061 tatagacaaa ataaaggcag gtattcgtag taataatggt catggtttgt ctttaatata
     3121 tacaaaattc acatctgctt atggaaataa ttttgatatg gatataaagt cgagtgataa
     3181 tgaaggaact tacataatga ttaaaattcc tattgaaaat tataaggagt atgtatagaa
     3241 cttgtgaaaa attaataagt tttataaatg ttaagaaata tattagatgt ttttatgttg
     3301 ttttatatat cttacttttt tatattatta agctaaaagg gaggtattct catgagaaga
     3361 gcactaataa tagatgatga agaagctgtt ctaaaaataa taacacattt tatagaaatg
     3421 aaagatatgc caataaaaat tgttggtaaa gcaacatctg gtgatgaagc agtagataaa
     3481 atcattggtt tagagccaga catagttttt atagatattc aaatgccaat atacaatggg
     3541 ttagaagtaa tagaaaaaac atctcattta tctaaaaaaa taaactttgt agtaataact
     3601 gcatttaact attttgaata tgctcaaaaa gctttgagat taaatgtaaa agatatactc
     3661 cttaaaccat tggatatgaa agaatttact aagtctgtag aaaaaattat tggatatcaa
     3721 tataccaata atgatttatt aaatgagata ctggaatata taaatctaaa ctatagcaat
     3781 gacttaaagt taaatgattg tgctagatta tttttaacca atccatctaa tatatctagg
     3841 atttttaaaa gtaatttaaa tacaactttt atatcatatc taaatcatat aagaatagaa
     3901 aaatctattg aattacttga aaatactaca aaaagtataa atgagattgc agaaatagtt
     3961 ggatataata gcctaaataa cttttataaa aactttaaga tagaaaaagg aatgactcca
     4021 aaagtttata aactgaacac taaaaattga aattgataaa taaaaagtca atatagataa
     4081 ttgtttgagt gtgtctgaat aagtatactt taattatgat aaaaattaaa gcgctttaaa
     4141 catatttaat aatattaaac cttataaaag ctgtacctat ttatataaaa aaatatatat
     4201 aaataaaaag aaaaagatat acaaaataaa aaaagtgaat taaatactgt ttcaaaaaga
     4261 cagaattttc atttaattca ctgagagttt atatttctta aaggaaggta actttataat
     4321 ggaggtaatt taatatgtca gacaaagtga atatgaaaaa tattatctgt attgcaggcg
     4381 cttatctagc aacagcaatt ggttctgggt ttgctactgg tcaagaaatt ctacaatttt
     4441 ttgcctcaca agggtatatg ggtttaattg gagcattaat atcaatggta ttgttttcat
     4501 ttcttggtgc tgaagttata actagaggta gagaattaaa acttaaagaa ccaacaagaa
     4561 tttacacttt ttattgtggg aagattttag gaaaatttta cgaatggttt agtccaatat
     4621 ttttatttgg gattcttata gtaatgattt caggtgcagg ctcaacttta actgaatact
     4681 atggtataaa tccttactta ggaagagtat tgatggctgt tgctacttta attacagtgt
     4741 ctatgggttt agaaaaatta gtaagcatac ttggacgtat tggaccagtg atagcagtat
     4801 ttacaattgc tgttggatta ataagcttgt ttaagcattt tgatggttta tctatggttg
     4861 gagaagctat gaaaaatatt gaagttacaa aagcatcaga aagtccaatc gtttctggtg
     4921 taatatataa tacattaaat attattgtaa tgattgtttt tttaacagga atgggagctt
     4981 ctattgaaag taaaaaggat gctttttggg gaggtttatt aggtggaata gtgtttatga
     5041 ctgctggtat agttatgtat ttggctatga tttcagatat aggaaatttg tatatgaaag
     5101 aaataccatc actttattta gcagataata tatctcctat tgtaggtgta tgtttttcag
     5161 ttgcattaat attaggtatc tatacaacag ctgttccact tttatggtct gtaaccaata
     5221 gaattgttga agatgaacat cctaaattta aattggtaac aacagtaata gctattttgg
     5281 cttgtatagg tggtttttta ccgtttggta aattagtaaa cattttatat ccatatacag
     5341 gttatatggg gcttttaatt ttagtctgta tggtgtataa agcaattaca tgtagaaaaa
     5401 aatcaacaca aagtcaggaa tagagtcacc tattaatagt ttttatttaa agaaattttt
     5461 tatataatcc gaatttaaaa agtcaaaata gttaaataaa ttgcaaaaca tgataatata
     5521 tttaattaaa ataatatagt aaaatataaa tataaactaa aattaaattt ataaaaaata
     5581 tctttataaa gattattaac ttatataatt tgtagttaaa tt
//
LOCUS       DACTGW010000220         5588 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.221, whole genome shotgun sequence.
ACCESSION   DACTGW010000220 DACTGW010000000
VERSION     DACTGW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.221"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            161..4414
                     /locus_tag="I9573_003125"
     CDS             161..4414
                     /locus_tag="I9573_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HAT4797721.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKISYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            4563..5057
                     /locus_tag="I9573_003126"
     CDS             4563..5057
                     /locus_tag="I9573_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAT4797722.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSVYIGDEKFNNLKNECIKK
                     GIDIKISKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKNIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            5173..5550
                     /locus_tag="I9573_003127"
     CDS             5173..5550
                     /locus_tag="I9573_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAT4797723.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT         2292 a          549 c         1028 g         1719 t
ORIGIN      
        1 atttaaggtt agaattagga attacctagt agagtaagct ttgattaata aaagaatcat
       61 aaaataaaaa tattagtcat aaagattata aataacttac ttaaaataaa gccatagaac
      121 ttacaaaaat taaaaaagca tataaatatg gaggggtaca atggatgaat tatttcatat
      181 aggaattgat gtaggttcta caacggtgaa ggttgtggta cttaataata gtaataatat
      241 agttcataaa gaatatagaa gacattattc tgatgtaaaa aaatcagtta aagaagtact
      301 aaatggtata tatgaaaaat tgggagatat aaatactacc attataataa ctggttcagg
      361 tggaattgga atatcaaaaa aattaggtgt aaaatttgtt caggaagtta tatctagtac
      421 aaaatcaata gagtattttc atcctgaaac tgatgttgtc atagaacttg ggggtgaaga
      481 tgcaaagata acatatctaa gtggaggaat agaccaaaga atgaatggaa tatgtgcagg
      541 tggcactgga gcattcatag accaaatggc atcactttta aagacagatg ccagtggatt
      601 aaatgaactt gctaaagggt ataatgttat atatcctata gcttcaagat gtggtgtttt
      661 tgctaaaaca gatattcaac ctcttataaa tgatggagcc aatcagactg atatagcaat
      721 gagtattttc aatgcagttg tcgtacaaac tgtgagtgtc ctttcttgtg gaagaaaaat
      781 agaaggaaat gttgcgtttt tgggtggtcc attgtacttt ttatctgaac ttagagaagc
      841 atttaaaaga gtattggatt taaaagatga aaacataatc ttcccagaaa atgcacaact
      901 ttatattgct attggagcag gattattatc agtagatgaa gataatatac aactaaaatc
      961 tctaatagaa aaattggata gtataaagaa tattgaagat ggagaagtaa atttattaga
     1021 acctttattt aaagataaaa atgagtatga agaattttca agaagacatg aaaaagaaaa
     1081 aataagttat gtggacataa attcaataaa aagcaattgt tatttaggaa ttgatgcagg
     1141 ctcaactaca actaaagcag cacttattga tgaggatgga agacttgtat attcttatta
     1201 taatagcaat gaaggaaatc cattaaaaac aaccattaaa gttataaatg aagtatatga
     1261 tatattacca aaaaatatta aaatattgag ttcaactgta actggatatg gagaaggtct
     1321 tattaaaaaa gctcttaaaa tcgataacgg agagatagag actatagcac attacaaagc
     1381 tgctaaattt ttcaataaag atgtagattt tatacttgat attggtggtc aagatatgaa
     1441 gtgcctaaaa ataaaagatg gagtaattga taatataata ttaaatgagg cttgttcatc
     1501 aggttgtggc tcatttttag agacatttgc aagttcttta tctatgacaa tagaagaatt
     1561 tgcacatgaa ggtatttatt ctcaaaatcc tgtagattta ggttcaagat gtactgtatt
     1621 tatgaattct agagtaaaac aagctcaaaa agaaggtgca agtgttggag atatatcagc
     1681 agggctatct tattcagtaa taaaaaatgc tttatttaaa gtaataaaga ttagagattt
     1741 aaatgagatt ggtaataata ttgttgtaca aggtggaacc ttttataatg atttggtgct
     1801 tagaagcttt gaaaaactaa ttggaaaaaa tgtaattaga cctaatattt caggaattat
     1861 gggagctttt ggttcagcac ttatagctaa agaaaaatat gaaagtggat acaaaacatc
     1921 cttgttatct aaggaagagt tgaataatat caagctagaa gcaagtgtaa ctagatgtaa
     1981 aggttgttca aatcattgtc ttcttacaat aaataaattt tctgataatg aaatttttat
     2041 ttcaggtaat agatgtgaga agggtgaagc tatttatggt gataaaaaaa taggattaga
     2101 aaagaaaaaa ccaataaatt tatttaagta caaatacaat agaattttta gatacaaacc
     2161 attggaaaaa gaagaagcaa aaaatgggga aatagggata ccaagagtat taaatatgta
     2221 tgaagattat ccattttggt ttactttttt caatgctatg ggctttagag ttgtactttc
     2281 agataggtca tcaaaacagt tatatgaaac tggtataact agtattgcat ctgaaacagt
     2341 ttgttatcca ggaaaattag ttcatggaca tatagaaaat ttaattgcaa aaggtataaa
     2401 aaatatattt tatccaagtg taacaaatga aaataaagag gattcaaatg cagataatta
     2461 ttataattgt cctgttgtaa tatcatattc tgaagtaata aaaaacaatg tagagaatat
     2521 aagaaataag aacataaatt atataaatcc atttataagt ctaaatgata aggaaaaatt
     2581 gaagaaaaga ctatatgatg aattatcaag acatttttct gatataaaaa taactaaaga
     2641 agaagttaat catgcagttg ataaagctac tgatgaacag aatttattta aaatggagat
     2701 acaagcagca ggtgaaagag cacttaaaga aataaaagaa agaaatatga aggggattgt
     2761 gttatgtgga agaccatatc atatagaccc agaaataaat catggaatgc cagagcttat
     2821 aaattcatta gatatggcag tgcttactga agactctata tgtcatttag ccaatgtaca
     2881 aagaccactt agagttgtag accaatgggt atatcattca agattgtata aagcagctag
     2941 ttttgtaaga gataaaaact atttggaact tgttcaactt aattcatttg gctgtggatt
     3001 agatgcagta actacagacc aggttcaaga aatacttaat gaaaaatcga agatatatac
     3061 tattataaag attgacgaag gcaacaattt aggagcagct aagataagga tgaggtcctt
     3121 aaaagcagct atgtttgaga gagaaagcaa aaatatagat gtaaaaaata taaaggtaaa
     3181 caggatacag tatgctaaaa ataatcagat aacgaacaaa catactatat tagcaccaca
     3241 aatgtctcca atacatttcc aattccttga aaaagctgtt aacttaagtg gttataatat
     3301 agaaattcta aaagatactg atagtagtgt aattgaagaa gggttaagat atgtaaataa
     3361 tgatgcatgt tatccagcta taatagttgt aggtcagcta ataacagcat taaaatctgg
     3421 caaatatgat ttaaataata cttctgttac tataactcag actggtggag gttgtagagc
     3481 tacaaattat attggattct taagaaaggc tatatatgat gctggattta aagatgttcc
     3541 tgtcattgca cttagtgtaa atggaataga agatagtggt ataatggata atatatcact
     3601 aaaattaatc aatagactct ttatgtctgt tgtttatggt gatttgctga tgaaagtact
     3661 atatagagta agaccatatg aaaaagttac tggaagtgca aatgctctat atgaaaaatg
     3721 ggttgatata tgcaaatctt ctcttgttaa agcaaaaatt tctgtattta ctagaaatat
     3781 aaaagatata gtaaaagaat ttgataattt agagatacta gatatcaaaa aaccaaaagt
     3841 aggtttggtt ggagaaatat tggtgaaatt ccatccaatt gcaaataata atttagtaga
     3901 tatattagag cgagaaggtg ctgaagcagt cgtgccagac cttacaaatt tcttcttaag
     3961 ttgtgccttt aatactatat acaaacacac tcatttagaa ggaagtagaa agagtagaat
     4021 gataggagaa gcatttatat acataactgg aatatatcaa agagtatata aaaaagcact
     4081 tgataagagt gagagatttt atgctccagc caatataaaa aatgttgcta aaagtacaga
     4141 accagtagtg tcattaggta atcaaactgg agaaggttgg cttttaacag gtgagatggt
     4201 tgaattatta aatgaaggtg tagaaaacat tatttgtatg caaccttttg gatgtttacc
     4261 taatcacata attggtaagg gttctataaa agaattaaag aggttgtata aaaatgcaaa
     4321 tataatacct attgattatg acccttctgc aagtgaggta aatcaactta acaggataaa
     4381 acttatgctt tctaaggctt ttaaaaatat ataagattta tattttgata tgttaaaagt
     4441 taatgaattt aaaaatatac tctatattaa gtatctataa aaaagagtta tcctattttt
     4501 tagggtaact ctttattagt taaaaaatta tatgtatact gatattaaag gggggaagta
     4561 atatgagttt agtatatgat gtttataaat caaaattagg aaatttgtac atattaagtg
     4621 aagatggttt tatagttagt gtatacatag gagatgaaaa atttaataat ttaaaaaatg
     4681 aatgtattaa aaaaggaatt gatataaaga taagtaagga agatttaaag gaagcaataa
     4741 gccagataga tgattacttt aatggaaaaa gaaaagtctt taatttaaat attcgtattg
     4801 aagggactga atttcaaaaa tcagtttata gggaaatgtt aaaaatacct tatggagaaa
     4861 cattgtgtta ttcagatata gcaagaaata taaacaatcc aaaatcagta agagcaatag
     4921 gacaagcaag taaaagtaat aaaattccaa taataatacc ttgtcataga gtagttggca
     4981 aaaaaaatat tggtggatat atggggaatc attctgattt gaaagagata ctattaaatt
     5041 tagaaagaca gagttaactt agaaagaaat gtttttattg ttaatatttg atataataat
     5101 agtaaaaaga agtatttttt acaaaaacaa tgaagaattt attttaattt acaaaaatga
     5161 gggggaattt taatgtgtga taaagttata gcatcaaaat atcatgagaa gggatttaat
     5221 tgtgcagaat cagttataaa agcatataat gaggaattta atacagatat accagtttgt
     5281 ttaggaagtg gactgggttc agggtgtggg gttgctagtt tatgtggtgc agtaaatgca
     5341 tcaaatataa taattggata cgtaaaaggc agaaaccacg aagatgaaag tactaaagct
     5401 aaagtatatg ctaaagattt aactacaaca gtaagaaaag agtatggttc agagttatgt
     5461 atagacctaa aaaaagattt agttgcttgt agagaaataa tggatttcgc atatgatagt
     5521 ctaaaggaaa ctttaaaaaa agaattataa gaaaataagc tatcaagtat aaattatttg
     5581 atagctta
//
LOCUS       DACTGW010000221         5569 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.222, whole genome shotgun sequence.
ACCESSION   DACTGW010000221 DACTGW010000000
VERSION     DACTGW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.222"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            457..1317
                     /locus_tag="I9573_003128"
     CDS             457..1317
                     /locus_tag="I9573_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAT4797724.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1307..3178
                     /locus_tag="I9573_003129"
     CDS             1307..3178
                     /locus_tag="I9573_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAT4797725.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEASNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3198..4652
                     /locus_tag="I9573_003130"
     CDS             3198..4652
                     /locus_tag="I9573_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAT4797726.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLMDLYLKYSETVI
                     RRFDDKVKYWITFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         2168 a          563 c          984 g         1854 t
ORIGIN      
        1 ataaaactaa atttgaagct ttacctatta atatatctta atagtgatta aataaaacta
       61 aacttaagaa tttatttatt aacatacagt tcattatatt taaaatcatt actatgataa
      121 ttcataatag taaaatagat aatggttcag acaatattgt aaatattagt gacagtaaaa
      181 tagtatcaat ttagtatata aactttgcca gataatcgca taacagagct tgtagttata
      241 gaataaaaaa atatctagga aaccgttgac agatttatat ggatagtata acatatagaa
      301 tatgaaatta aacaaaaaag aattttaatt atggcgacgt agtcataatt ttgagtatgt
      361 tactgattag caggcagaac tcaagtatat atgtgaaaaa tagagggata ctctatacat
      421 atatgcttga gttcttttta ttgaaaggag gaatatatga aaataattca gattttaaat
      481 aataatgtag ccttagtaaa aagaggtaaa aatgaaatta ttgtatttgt taaaggaatt
      541 ggctttaaaa aaagagtggg acaagtaata aatgaaaatg aaatagagag aacatatata
      601 ctagattcat acgatatgct agaacacttt agttatctac tacaaaactc agactccaat
      661 aatatagttc ttattaatga aataattgaa tatggtgaga aagctttaaa tataaagtca
      721 agtgattatc taagcttaac cttactagac catttagagt ttttacttaa aagatgtgaa
      781 aagaatcaat ttataaaaag tcctttggta tggaatgtta aaagatttta tccaaagcat
      841 tttgaaattg gaatgcatgc actaaaatta attggtgata gtaagggaat taatcttcct
      901 gatgatgagg cagtatcaat agcacttcat tttgtaaata tggaggtaaa taaagagtct
      961 catgatagta caattgtaga gttgagaact ttagcagata tagtttctat aataaaatat
     1021 cattttaatg tagagctaga tgaaacttca acaaattata tgagatttac tactcatcta
     1081 cagtacttta tccaaagaat tatgtcagat gaagcttcaa tagatgaaga taattctata
     1141 gatttatata aacaagtaag tcaactttat ccaaagtcat ttggaagtgt tcaaaagata
     1201 agaaaatata taaaatcaca atttaatgtc gatatatcta taagtgaaga aacttattta
     1261 atgttgcata taaatagggt gactcaaaga ctggaggcaa aatcagatga aatataaaga
     1321 tttaaatgaa aatataataa aacttatagg gggaaaagaa aatataaagt cggtagcgca
     1381 ttgtgttact cgtttaagac ttactttaaa tgatagaagt atcgctcaaa cagaagaaat
     1441 taaaaaatta aaaggagtaa ttgatgtagt ttctaatgat gttgcttatc aaattataat
     1501 aggtactcat gttgtagatg tatataacga gtttatgtct atgataggtt taagttcaag
     1561 tgaatcaagt ataaaaagtg aaagaaaaaa gggaattaga ggtatcaaag gtatatttaa
     1621 ttccatcttt gtggtagtct cagagactat gacttcagta attgaagtac tacttgcagc
     1681 tggtatactc gcaagtatat tagcactttt aaatatgtct ggcatacttc agagtgatag
     1741 ccctacatat caaatattag atacattaag aagtgctaca tttcacttct taccagtatt
     1801 aattgcaata gcatcagcaa aaagacttaa tgtaaatccc tatatagcag tagtgttagc
     1861 agttacgtta ttatcgtcat caatagatgg tgtagaagga ttaagtttat ttggtataaa
     1921 tttacaggct acaacttatg caaacagttt tattccaata cttttaggag tatgggcaat
     1981 gggaattata attgaaggtt tgaaaaaaat attacctaaa gcactacact attttttagt
     2041 tccagtgtta tctttaataa taaccctatc agttacatta actgtatttg gacctattgg
     2101 tatgtggatt ggtgatggat taaactatgc atgtatgttt ttaattgata cattgggtaa
     2161 ttggagtgtt gttgcactgt atgcagcttt acaaccactc ttaatagttt ttggtgcagc
     2221 aaactttact atgcctatat ctttaaattc tgtgacaaca ttggggtatg acccaatttt
     2281 tatgggcgct gcaacaatat ctgatattgc agtttgtggt gcaatgtttg gatatttctt
     2341 aaaaactagg aataaagaac aaaaacaatt atttgggaca gtaagtttta gtgcacttat
     2401 gggagttaca gagccttcag tgtttggtgt gtttatgaaa tatagaagac catttgtagc
     2461 agtaatgata ggtggaggta taggtggttt aatagcaggt ttagctcaag ttaaaacata
     2521 tggaatggtt tggggattag ctgcattacc aacatatctt gttggaggag aggcatctaa
     2581 ctttacattt atgataataa gtgtagtagt atcatttgta gcagcaacag cagcttccta
     2641 tttactagga ataccttcag atgaaaaaga agaagttaag ggtgataatg aaaaagaatt
     2701 agaactaata atcaacgaga acaattcaag aactattagt attggaaatg ttgcaaaagg
     2761 tcaggtaatt gcacttgggg aagttaatga caaagcattt tcctctggag cattaggtaa
     2821 aggggttgga gttataccta gtgaatcaaa atcaactgtt atatctcctg tagatggaac
     2881 tataactata gtatttccaa ctaaacatgc atatggtata gtgactgatg aaggattaga
     2941 aatattaatt catattggta tagatactgt aaacttagat ggaaaattct ttgaaagtct
     3001 tgtaacacaa aatcaaaggg ttaaaaaagg agatgcttta gcagtatttg aatctgataa
     3061 aattatagag gaaggctttg attctactat aataactgta gttacaaata ctagcgatta
     3121 tttagatgta atctcatcaa atgatgaatc agaagaatta ttaactgtaa tagtataaat
     3181 tttaggaggg aaaattcatg aatattaaag atacattttt ctggggagga agtattgcag
     3241 cccatcaatg tgaaggttca tgggatagtg ataacaaagg acctgcaatt atggattttg
     3301 taactaaggg ctcttatgaa acgccaagag taattactga taaaatagaa gaaaaattgg
     3361 attatccatc tcataatgga atagattttt ataatagata taaagaagat atagctttat
     3421 ttaaagaaat gggatttagt gcattaagaa tatctataga ttggtcaaga atttttccaa
     3481 atggagatga tgaaaatcct aatgaattag ggattaaata ctatgaaggt ctgatagata
     3541 cattaataga aaataatata gaacctattg taacactata tcattttgaa ttacctatga
     3601 atttagtaca taaatatggt tcatggaata acagaaaact tatggatttg tatttaaagt
     3661 acagtgagac tgtaataaga aggtttgatg ataaagtaaa gtactggatt acatttaatg
     3721 agatgaacca tattgaccct caaacagagc actcggatat ttttacttat atgatagcag
     3781 gtattaagta tagtgaaata gaaaataagg ttcaaacatt ggcaaatgta gggtataata
     3841 tgactttggc gggtgtaaag gttgcaaagt tggctagaga aatcaatccc aacaataaag
     3901 ttggatgtgt atttggacta aatccaattt atccatacaa ttgtaatcct agtaatgtgt
     3961 taaatgcatt tttagataat gatcgtgatt attatcaaat tgatgcaatg tgcaatggga
     4021 agtttcccca atataaatta aaagaatatg aaaaaaataa tataactatt gaagtgactg
     4081 atgaagacaa aaaagcattt aaagaaggta agttagattt tataggtctt aattattatt
     4141 tttcaagtgt atctactcca aaagaaaatt tatcaggtga taaatcttta tttggtggga
     4201 ttcaaaatcc atatttggaa caaagtaagt ggggatgggc aatagaccct ataggtttaa
     4261 gatttacctt gaactatatt tacagaaagt atcaattacc tatcttaatt acagaaaatg
     4321 gtttaggggc atatgataaa atcgaagaag atggaactat aaatgatgat tatagaattg
     4381 aatatttaag taagcatcta tctcaaatga agtctgcaat tgaagaggat aatgttgaat
     4441 gttttggata tttgatgtgg ggaccaattg atttagtcag tgcaactaca ggtcagatga
     4501 gtaagagata tggatttata tatgttgact tagatgatga aggcaaagga agttttaaga
     4561 gatataagaa aaaatcattt gattggtaca aagaagtaat aaaatctaat ggagaagttt
     4621 tagatttttc tacggatata gtagtagggt agtaataaga taaatagaag ttaggaacta
     4681 atgttaatta gtgtctagta aagcatttaa aatagagtat agggtcttta aagtaaagtg
     4741 tgtattctta ttaaaagtaa tggcaactaa aattactata aaaaggagaa tacacatttt
     4801 ttatgtttat acttaagtaa atatttagac atattcatag tataatttac tcattatata
     4861 aaaatagtat gatttactca ctatataact ttataaattt tctagaaaaa tgtcagaata
     4921 tattgacaaa aaaaagattt gaaattataa tctatattga gaatagtttg acaatataaa
     4981 aacaagtatt tttacaaata gtgaaattat ttttattgtc aatttaagaa taatttagga
     5041 ctactataaa gatgaaggta tagatgtcat tatatgataa atcatacttg tatagaaaga
     5101 attttaaagt atgcttatca ttgatttaat ataataaata atttttgttt ctagtaatag
     5161 ttcataggaa taagatatct aaattcgctt agaaatagtc taatttacaa aggaggggat
     5221 gcactagtag ataaaatttt tatataaaga agacttaaag tggagtatat tctgataata
     5281 ttggtttaat ggttatatta ttatttcgtt ttagatattt taagaataat ttaagacgtt
     5341 tgtaaaaatg tattctatat ctaatattta aataaatata agtcttccaa catgtatact
     5401 aagatagaaa gtagttgaag tacaattcta acttgtaaaa ttatgtagaa agtaaaaatc
     5461 aaaaagaaat ctagtaagtt gataattata gttaatattt agagagttaa ctctagttat
     5521 atttatttag atattttagg aataatttaa gacgtatata atatcatca
//
LOCUS       DACTGW010000222         5546 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.223, whole genome shotgun sequence.
ACCESSION   DACTGW010000222 DACTGW010000000
VERSION     DACTGW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.223"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            452..1861
                     /locus_tag="I9573_003131"
     CDS             452..1861
                     /locus_tag="I9573_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HAT4797727.1"
                     /translation="MIFSSLIFLFLFFPVTLICYYLANPKYKNVMLLLASIAFYAWGE
                     PKYIFLMILSIIVNYIFGIKVSGSKNKKMWVVLSVIYNISILAVFKYSNFFIDNINFL
                     FNTDITIPEVKLPLGISFFTFQIMSYVIDVYRNDAKVQRKLANLALYISLFPQLVAGP
                     IVRYQTVADSIEYREHNWDKFTQGVNRFVVGLGKKVILANQLGVIADGVFNKSAGDLS
                     RLEVWLGIVCYTLQIFYDFSGYSDMAIGLGKMFGFEFLENFNYPYISQTVSEFWRRWH
                     ISLGTWFKDYVYFPLGGSRVSKAKLYRNLFVVWFLTGMWHGASWTFIIWGLYYGILIS
                     VEKAFLEKFLYKMPRIVRHIYLLLIVMIGWVFFRVDDLPAAIKFIGIMFGAGNYPVIS
                     DAFNVFIIEYWYIIVGAILLSTPIVKVVKSLLIKKNKNTLNSTLYQVTNVVFIVSCMF
                     IVIIMLSSSTYNPFLYFRF"
     gene            1877..3079
                     /locus_tag="I9573_003132"
     CDS             1877..3079
                     /locus_tag="I9573_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797728.1"
                     /translation="MSKKKPRIVAISFLIIVFGFFILNFTLPKKKLSITENRNLAKFP
                     TVENLKKCDFIAKFEEYFNDHFAFRDEMISINTRTQANLNKTKVGNYYLGKDNWILGM
                     FPEILDQKQLSRYEKSINYLSNLSKDMGKNVYFTMMPHKTNMLKHLYPKYVDNMDNID
                     INLKNFKNRLNPDLIKYIDMDEYFLSNYSDKERENLYFKTDHHWTGYGAFEGFKMMAS
                     NMDLGLSKQDLDKHFSSYKKSIITDKKFIGSYNQNIDMLIKENEYVVYMTKDNQDYSF
                     DINGEEKEEKEEKEVYATERDNKEWDYGGAYIRGAKTNILTIKNPKALVDKKILIFRD
                     SYQAPTTLLFADMFKEVEIADPRNINSIYKTYKEIIEESNADIVMFMYNSSGFDSMID
                     SMIDKGIK"
     gene            3127..4263
                     /locus_tag="I9573_003133"
     CDS             3127..4263
                     /locus_tag="I9573_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797729.1"
                     /translation="MFIFYCFGKNKLIKIKVRRCSILNKRHSLPIVSVILLMVLIIFN
                     NNQNYITMKEVFNVHIMQSQNKKIKQKYYLTKDGWLLGNFAKLLSDYHIKSYSKAINN
                     LSKLNRKKDFYFVSLPHKTNMLKHLYPSGIEGKNNIDINKENLEKQLTDINFLDIDDY
                     FYRSFTEKEREKFFLKTDHHGNSLGAFEEYKFILDKLDLGLSKSEIDSHLKRYSTRYV
                     NDKVFIGSYNKKMGYPLKKKESISYVEISDANYKYFISNGTKNTEINENIIKAKLKDK
                     REWDYGGAYMRGCDCHILKIRNSSSLTNKKALIFRDSYQAPMTWMLADTFKELEILDP
                     RYIKNIDMTYDEIIKNSNANVVLFMYNSFGFAGMIQEMIDKGIA"
     gene            complement(4713..5156)
                     /locus_tag="I9573_003134"
     CDS             complement(4713..5156)
                     /locus_tag="I9573_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797730.1"
                     /translation="MKKNMLYLGIVYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GICMIFQYMYWSKPERAVDYEEKIKNQRIEMNDERRVMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(5315..>5546)
                     /locus_tag="I9573_003135"
     CDS             complement(5315..>5546)
                     /locus_tag="I9573_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT4797731.1"
                     /translation="QLKYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREW
                     ICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         2160 a          686 c          765 g         1935 t
ORIGIN      
        1 aataattaaa ataaaattta taatcaatca taattattta gtataatgat tcgatattat
       61 tttaaagtat ttgtcattta aaacaaactt gcgataaaag catatctgaa agtattctga
      121 ttctaatact taataaagag ttctttttac agttatagat ataaacaaca tctatcttaa
      181 tagactgtat agaggcacaa tcaaaggtaa acacatatag ttaagtaata tgtccttgat
      241 ttttctttat tataatagct ttattaattc attttaatat tacatcattt taaaaaaatt
      301 atatgaaaag tcaaaattta catatagatt aattaaactt gtaaaaacac atataaattc
      361 atacaattaa gtacttaaaa cttgaaaata cttactttgc tatatatttt agataaagat
      421 ataattgatt tggttaagaa aggagtctaa tatgatattc agtagtttaa tatttttatt
      481 tttattcttt ccagtcacac tcatttgcta ttacttggca aatcctaagt ataaaaatgt
      541 tatgttactt ctagcaagta ttgcattcta cgcatgggga gaaccaaaat atattttttt
      601 gatgatacta tcaataatag taaactatat atttggtatt aaagtttctg gcagtaaaaa
      661 taaaaaaatg tgggttgtgc tttctgttat ttacaatata tctattcttg ctgtatttaa
      721 gtatagcaat ttttttatag ataatattaa ttttttattc aatacagata taactatacc
      781 tgaggttaaa ttacctttag gtatctcatt tttcacattc caaataatga gctatgtaat
      841 agatgtatat agaaatgatg ctaaagttca aagaaaatta gctaatttag cattatacat
      901 aagtttattc cctcagcttg tagcaggtcc tattgttaga tatcagactg ttgcagatag
      961 cattgagtat agagaacaca attgggacaa atttactcaa ggtgtaaaca gatttgttgt
     1021 tggtcttgga aaaaaagtaa ttctagcaaa tcaattaggt gtaatcgcag atggtgtatt
     1081 taataagtca gctggagatt tatcaagact tgaggtatgg ctaggtatag tttgctatac
     1141 acttcaaata ttctatgact ttagtggcta tagtgatatg gccattggtt taggtaagat
     1201 gtttggattt gagtttttag aaaatttcaa ctatccatat atctctcaaa cagtatctga
     1261 gttttggaga agatggcata tatctttagg tacttggttt aaagactatg tgtacttccc
     1321 attaggtgga agtagagtct ctaaggcaaa actatataga aatctatttg tagtatggtt
     1381 tttaaccggg atgtggcatg gagctagttg gacttttatt atatggggtc tatattatgg
     1441 aatattaata tcagtagaaa aagcattctt agaaaaattt ctatataaga tgccaagaat
     1501 tgtaagacac atatatctac ttcttatagt catgattgga tgggtatttt ttagagttga
     1561 tgatttacca gcagctatta aatttatagg tattatgttt ggtgctggaa attatccggt
     1621 tatcagtgat gcattcaatg tatttataat tgaatattgg tatataattg ttggggcaat
     1681 ccttctatca actcctattg taaaagttgt aaaatcatta ttaattaaga aaaataaaaa
     1741 tacattaaat agcacattat atcaagttac taatgtggtt ttcattgttt cttgtatgtt
     1801 tattgtaata ataatgttaa gtagctcaac ctataatcca ttcttatatt ttagattcta
     1861 attgggaggt ataaacatga gtaaaaaaaa accaaggatt gttgccattt catttttaat
     1921 tatagtattt ggatttttca tactaaactt cacacttcca aagaaaaaat taagtataac
     1981 tgaaaataga aatcttgcaa aatttccgac tgtagaaaat ttaaaaaaat gtgactttat
     2041 agccaagttt gaagaatatt ttaatgacca ttttgcattt agagatgaaa tgatatcaat
     2101 aaatacaagg actcaggcta atttaaataa aactaaggta ggtaactatt atctaggaaa
     2161 agataactgg atattaggta tgtttccaga aattttagac caaaaacaat taagtcgata
     2221 cgaaaaatcc attaattatt tatctaatct aagtaaagat atgggaaaaa atgtgtattt
     2281 tacaatgatg ccacataaaa ctaatatgtt aaaacatttg tatcctaaat atgttgataa
     2341 tatggataat atagacataa atttaaagaa ttttaaaaat agattaaatc cagatttaat
     2401 taaatatata gatatggatg agtatttttt aagtaactat tcagataaag aaagagaaaa
     2461 tttatatttt aaaactgacc atcattggac tggatatgga gcatttgaag gctttaaaat
     2521 gatggcatca aatatggact taggtctatc taagcaagat ttagacaagc attttagtag
     2581 ctataaaaag tctataatta ctgataagaa atttatagga agttataatc aaaatataga
     2641 tatgctaatt aaagaaaatg aatatgtagt ttatatgaca aaagataacc aagattatag
     2701 ctttgatata aatggagaag aaaaagaaga aaaagaagaa aaagaagttt atgcaactga
     2761 aagagataat aaagagtggg attatggtgg tgcatacatt agaggagcta aaactaatat
     2821 attaactata aaaaacccta aagcattagt agataaaaaa atactaatat ttagagattc
     2881 atatcaggct cctacaacac ttttatttgc tgatatgttt aaagaagtag agatagcaga
     2941 ccctagaaat ataaactcaa tatataaaac atacaaagag ataatcgagg aaagtaatgc
     3001 agatattgta atgtttatgt ataatagttc tgggtttgat tcaatgatag attcaatgat
     3061 agataaagga ataaagtagt aaataaaaag cctctattaa attcaaaatt taatagaggc
     3121 tttttattgt ttatttttta ttgctttggt aaaaataaac taatcaaaat aaaagtaagg
     3181 agatgtagca tattgaacaa aagacatagt ctacctatag tatcagtgat attattgatg
     3241 gtcttaatta tctttaataa taaccaaaat tatattacaa tgaaagaagt ttttaatgtc
     3301 catattatgc agagtcaaaa taaaaagatt aaacaaaagt attatctaac taaagatgga
     3361 tggctattag gcaactttgc taaattatta tctgattatc atataaaatc ttactccaaa
     3421 gccattaata acttgagtaa gttaaataga aaaaaagatt tttactttgt ttcacttcct
     3481 cataagacaa atatgcttaa gcacttgtat ccaagtggta tagaaggtaa aaataatata
     3541 gatattaaca aagaaaactt agaaaagcag ttaacagaca taaatttctt agatatagat
     3601 gattactttt atagaagttt taccgaaaaa gagagagaaa aattcttttt aaaaacagac
     3661 catcacggaa attcattagg agcatttgaa gaatataagt ttattcttga taaattagac
     3721 cttggcttat ctaaatcaga aatagatagt catttaaaaa gatacagtac tcgatatgta
     3781 aatgacaagg tctttatagg tagttataat aaaaagatgg gatatccact taagaaaaaa
     3841 gagtctataa gctatgttga aataagtgat gcaaattaca aatattttat tagtaatggt
     3901 actaaaaata ctgagattaa tgaaaatatc ataaaagcaa aattaaaaga taaaagagaa
     3961 tgggactatg gtggagctta tatgagaggt tgtgattgtc acatacttaa gattagaaat
     4021 tcaagctctt taactaataa aaaggccctt atatttagag attcgtacca agcacctatg
     4081 acttggatgt tggctgatac ttttaaggaa ctcgaaatac tagatcctag atatataaaa
     4141 aatatagata tgacctatga tgagataatt aaaaacagta atgcgaatgt agttttattt
     4201 atgtataata gctttggatt tgctggaatg atacaagaaa tgattgacaa gggaatagca
     4261 taacaatttg aatcagctcc tttttataca aaaaatagtt ctcaataatt cctaatctat
     4321 atgataattt agtacatctc tttaacaata aaaataaaat cacaaaaatt cttataccat
     4381 cacttatctt tatgataaat tggttgagaa aaaattttat aatttatatg atatgcaatc
     4441 ttaattaatt ctttatctac tgcaaccttt aataaaagaa accatccaag actattaata
     4501 tgaatttata taaaaattac tttcaaataa agtactgcag tatttttatt taaagtatct
     4561 ataatcttta accacaaaaa gccacaaaat acaatgtata cttatggctt tttataaatt
     4621 tatataattg tatataaaga tatattctaa aactgtctta agaatctaaa gtttaaaaat
     4681 ccaatatttt tattgtgaat ttaaactttt tattacaact ttttatctaa tttattgtat
     4741 acaacaacac tacaaataaa ttgaaataaa ataagcaatc caagaacaac taaaacccac
     4801 ttgcttatta ccattacaga aaatacactc aatatagaaa caataaatat aagaattaca
     4861 agaacatagg acatgatttg attagtgata cgtccagctt tatcacgtaa cataactctt
     4921 ctttcatcat tcatctctat cctttggttt tttatctttt cttcataatc tactgctctt
     4981 tcaggcttac tccaatacat atattgaaaa atcatacata tacctggacc taaagcagct
     5041 ccagataatc cccaaataaa agcctcaaat gaaaactcag taaacgtagc caataaaaca
     5101 agtaaaattc cacaaataaa atatactatt ccaagatata acatattctt tttcattttt
     5161 actcctcctc ataaataaat atttcttcaa tactcatctg aacctcccat gactaaagtc
     5221 acagggttcc tgcttcacag aattttgcat actaaaatat gtcttactaa ttctccacag
     5281 gccatccccg tagttcctac ggttcttaca tacgttattt attttgaatt gttattaatc
     5341 ttaatccttc ttttaatata tttatacttg catttatatc tctatcatga gtttcattac
     5401 aactaggaca aatccattct cttatattta agtccttaac ttcctcgttt ttatacccac
     5461 atttattgca tatttgtgaa cttgcaaaga atttgcctac ttttacaatt tgtctaccat
     5521 accaattagc cttgtatttt aattga
//
LOCUS       DACTGW010000223         5542 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.224, whole genome shotgun sequence.
ACCESSION   DACTGW010000223 DACTGW010000000
VERSION     DACTGW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.224"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            45..830
                     /locus_tag="I9573_003136"
                     /pseudo
     CDS             45..830
                     /locus_tag="I9573_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895703.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1736..2443
                     /locus_tag="I9573_003137"
     CDS             1736..2443
                     /locus_tag="I9573_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAT4797732.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            3055..3555
                     /locus_tag="I9573_003138"
     CDS             3055..3555
                     /locus_tag="I9573_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4797733.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(3805..4584)
                     /locus_tag="I9573_003139"
     CDS             complement(3805..4584)
                     /locus_tag="I9573_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797734.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKALDKENNVLGKSKALSFEYKNGNDEISLDTANNVLEKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(4581..5060)
                     /locus_tag="I9573_003140"
     CDS             complement(4581..5060)
                     /locus_tag="I9573_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAT4797735.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRTLLKTKLENTEVII"
BASE COUNT         2103 a          572 c          820 g         2047 t
ORIGIN      
        1 atttagaatt taatattaaa aatattaatg atgattggag ttttatgaat tttgaaaatg
       61 attttatcac tccagaattt agaaaaaagt acatatgatt agtattagat aatgctcaaa
      121 atattaataa agtatacaca actacttttc ctgataaaga aataattaag caagttatag
      181 ataaagatga aaaagatatt ttaatttttt cacatcacgc aatgggatac atagcatcag
      241 atgaaggttt tccatttcac gacataccac tatcttatat ggaagagatg aaaaatcgta
      301 gaatttcttt ttatgtatta cattctccac tagacaacta tagtgactat tcaacttctg
      361 ttagttttgc aaaagctatg ggtttagaaa tagtcaaacc attttgtaaa tatgacgata
      421 aaattgaggt aggagttata tgtaaaacat cactaaaatc aatagaagag attaaggaat
      481 taattaaaaa atctgttggt catgatgtga aattgtatga ttatggagat tctgtattaa
      541 aagatggttt agttgctatt gcagcagggg gaggaagtta tccatttgta gcaagagaag
      601 ttgctgaact tggtataaat ctatatatta ctgggtttac taagccttta aaacattttg
      661 aaccagtatt agagttccat caaatagcaa aagataattt aattaatgtt ataggtgcta
      721 ctcattattc tacagaaaaa tttgcgtgta tgggcatggt tgattatttt aaaaatctag
      781 gtttagaatc agagtttttg caaggaaaat attacttaga ggatttatag gacatattaa
      841 acaataaaaa taaacagtta gtaaaaattt attttactaa ctgtttattt ttttacctat
      901 ttatccagtg ttttgaaaaa atctatcttt attttttaaa ctaagaaaat agacttttaa
      961 ttgttgatta gaagatttag ttttgatgtc ttttaaatta tttttcgata ttttttagtt
     1021 tattttttga tgtattttag attattattt aggattgatt agaatttata aatatttatt
     1081 ttagattatt gtaaatattt atttaaaaaa aatagaatta ttatatttat gataataaat
     1141 ggtataataa aaggtaaagt gtcaaatatg cactaaatta aataagttaa ttagtatata
     1201 ggttaaagtg gaagtaatta aaacttatta agtattagaa aatgacttct tattagagta
     1261 agtatctaaa atgatataaa aattatgatt actcattaaa gtgactatta tagagtagat
     1321 aaaattgccc ataaaaatag ctataataga gtggataaga ttactcatta aagcaattac
     1381 tatagagtag ataaaattac tcactaaaat aactataata gagtggataa gattattcat
     1441 taaagcgatt actgtggagt agataaaatt actcactaaa ataactataa tagagtagat
     1501 aagatactca ttaaggtgat tattgtagag tagataaaat tacccatcaa agttaattat
     1561 cacatattag ataagattat tcattaaagt tactatcaca gagtggataa aattacacat
     1621 taaaattgtc tgttaaagaa tgaataaaaa agagattgct caatataaga agcatataaa
     1681 aaatcaaaca tataaaattt aataaataat aactgatagg gaaaggaaag atagaatgat
     1741 atttacaaaa agcaataaat acgataagga ctttctcatg aaaaatatga tgggacctaa
     1801 ttgtataaaa atattggaag aattaacaag taagataaaa ctagaaaagg gaatgcgtat
     1861 tttagactta ggttgtggta agggtatatc ttcgatattt ttagcaaagg aatttgatgc
     1921 tactgtattt gcaacagatt tatggataga accaactgaa aactataaaa gatttaaaga
     1981 gtttaaattg gatgataaaa tatttccaat acaggcagag gcacatgaac taccttatgc
     2041 agaaggtttc tttgatgcgg tgataagtat cgattcttat cactattttg gaaacaaaga
     2101 aggattttta gacaatcata tatcacctct tgtaaaagag gggggaatac ttgctatggc
     2161 aatgcctgga ttaaaagaag attttgtgga ttgtatacca gatgaactca ttccattttg
     2221 gcaagataat atgaacttcc attcaattac atggtggaat aaactttggt ctgaatcgga
     2281 gtctgtgatt gtagagaaat gtgaagcttt aaattgtcat gatgaagctt ggaaggattg
     2341 gataaattgt gataattcat atgctataaa tgacaaaaaa atgatggaag tagagaatgg
     2401 aaagtatttt aatacaattt ctttgatagc gagaactaaa taattgtgat gaatttataa
     2461 aagtataaga taagtagaaa tataagttga atgagagtat aagaagcata gaaatataag
     2521 ttgaattaaa gtagaaggta aataaaaatg taagttaaat aaagagttta tacaaaaatg
     2581 attttttaga ttatttatac ccaatcgagt tgcttaggtc agttgggtat ttttattttt
     2641 ttaaagttaa attgcaaata ctattgtgtg atatatattt taatactaaa tgtttgtttt
     2701 ataagaaata aactaataga aaaaatagtt ttaacaatga aagcaaaaaa ggtattgatg
     2761 aagattaaaa attttatagt accattttat atattttttt atatatttta ttatacttta
     2821 aatcaaattt caaaatgatt aagtatatat acccaaaaga ttaaagaact atatttatta
     2881 tactattaaa aattatttta atagagatac aagagataga tatattggtt ttaattttat
     2941 agaaatatac aaagatattt taagctaata tatagtaatt tatatagcat tataaactag
     3001 aatcatataa aataagttaa ctacaatcat aaattaaaaa ggagaaatgt taagttgata
     3061 gaaggaataa aatttataaa ggcagaagaa aagtatattt attcgtattg gcaaactttt
     3121 gataaaatag caaaagagag gaaatatcta gctatggatg aagcctttcc ttttgaagaa
     3181 acagttgaat ttataaaaaa tactataaat aaaaacttac cacaactgtt tataattgat
     3241 ttagaatcag ataactgtat tggttggtgt gacgttttac ctaaaactga aaaagttggt
     3301 tatttaggca tgggtattct taaagagtat agggaaaaag gtatcggaag tagtctttta
     3361 aaacaaatta ttgatttatc aaaagaatat gggtatgaaa aaattgagct tgatgtattt
     3421 aaaagtaata gtagagctat tcatgtttat aaatctttag ggtttgtaga ggtaaataca
     3481 atatctagtg gttttacatg gaatgataga cctgtaaaag aagaagtaat tcaaatggag
     3541 cttacactta tataaaataa agttaaagtc tttaaaattc catatacact tgtataaaat
     3601 aaagttaaag cccttaaaat tccatataaa agaaatattt ttaagggctt tttaataatg
     3661 gttcttttga aggtagctaa atatctaaaa ttttatatat taaatttaaa gaaaatataa
     3721 agatggaaga tacaaccatg tttactaata aaaaaattaa agtcataaat aataaactca
     3781 taaacaactt attattactt atgtttattt tgcatctatt acttcatgag ccattttaac
     3841 taagtcattc atagataaat ttgagtcttg aactgtaaaa gaatagtaaa ctccatcttg
     3901 tttccatgtc aaaaacttaa attcattaac ttcaactttg tctgattcat tatttggccc
     3961 tacactaaat gtgtatttgc ctgatttttc atcaatttta tcctgttctg tcattttata
     4021 accttctggt acaaactttt gtgcgaatgc ttcataagaa atattaatat ctttgtaagt
     4081 atctacaact tttagattgt cagaactctt ctcaagtaca ttatttgctg tgtcaagaga
     4141 tatttcatca ttgccatttt tatattcaaa actaagtgct tttgattttc ctaaaacatt
     4201 attttctttg tcaagtgctt ttttatttac agtatatgct cctttaaatt tatatccatt
     4261 actaaattca gaaactatat taggggtaaa gttaaagtct ttttttactt gttctgtagt
     4321 tgggatagat gtataggtag ccttagtact tgattttcca actaaactat atatatgtcc
     4381 tactgcaagg gctgttgtgc tcaatatcaa agtaatggct aaagttgcaa ctaagccttt
     4441 tctaaatggt aatttgtgtg ttttattatt aatcatttta taattttcct ctctttctgg
     4501 taaactattt agtgttttac ttaaactatt atgaaaactt tctggaactt ctggaaacga
     4561 attatttaat atatttttat tcatatgatt acctccgtgt tttctaattt agtttttaat
     4621 aatgttcttc ctcttgataa tctacttttt acagttcctt gaggtatatc taatatttcc
     4681 ttaacctcat ctactgaata accttcaata tagtaaagga ctattggaat tttaattttt
     4741 ttaggtagac tttgaatagc agtatacaaa tcgctatagt catctttttc atcaattttt
     4801 ttgtttttga agacatcttc aaatgataga gattttaatc gtttgcgtct taatgaatta
     4861 cattcattta tgactattct aatcagccat gttttaaagt attgttcttc cttaagagaa
     4921 tccaactttt catatgcttt taaaatagca ttgttgacgg catcttcaca gtcttggtcg
     4981 tttcctagaa tagacttaga tactcgatac aaagtctgtt ctgattcaag tatattgttt
     5041 gtaaatgtag ttttatccaa ctgagttcct tcccttctat gcaaatcgta gcgctacttt
     5101 tttgcctttc acttattaga taaaatttta ctatagatgg ttcacttttt ttatgattat
     5161 ttgataaatt taagtttgaa agttttgcct attctataca aatattctat aatatatttt
     5221 tttctagtat tttcccctca tatataaagc catgtagata tattaaactt tagaagccat
     5281 ttatatattt agttttatag taagtgttta tattaagtaa ttataaaata gtaagtattt
     5341 gatgttgagt agttagaaaa taatgctttt ggattattta cttcatgtat agatttaaaa
     5401 taagtttttt aattaatgac taaagataac ggtattttga ctaaaagtag gcttttaaga
     5461 aaaaacaatg aaacttaaat gagaatcaag agttagaaat gtcattattt tagatagata
     5521 tgaacataaa taaacttaaa aa
//
LOCUS       DACTGW010000224         5513 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.225, whole genome shotgun sequence.
ACCESSION   DACTGW010000224 DACTGW010000000
VERSION     DACTGW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.225"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            70..522
                     /gene="accB"
                     /locus_tag="I9573_003141"
     CDS             70..522
                     /gene="accB"
                     /locus_tag="I9573_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAT4797736.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            538..1899
                     /gene="accC"
                     /locus_tag="I9573_003142"
     CDS             538..1899
                     /gene="accC"
                     /locus_tag="I9573_003142"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAT4797737.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            1914..2768
                     /locus_tag="I9573_003143"
     CDS             1914..2768
                     /locus_tag="I9573_003143"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAT4797738.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKELLY
                     QILAMHKK"
     gene            2789..3733
                     /locus_tag="I9573_003144"
     CDS             2789..3733
                     /locus_tag="I9573_003144"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HAT4797739.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            3922..4182
                     /locus_tag="I9573_003145"
     CDS             3922..4182
                     /locus_tag="I9573_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HAT4797740.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            4335..4706
                     /locus_tag="I9573_003146"
     CDS             4335..4706
                     /locus_tag="I9573_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HAT4797741.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIEDACKILSCNGYEVE"
     gene            4736..>5513
                     /locus_tag="I9573_003147"
     CDS             4736..>5513
                     /locus_tag="I9573_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797742.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFI"
BASE COUNT         2132 a          575 c          997 g         1809 t
ORIGIN      
        1 ttttctttta taaatttgtt ttttgtgata taatattagc accaggtaat aatacaaggg
       61 ggtaacaaaa tgaacatcaa tgaaattaag gaattattaa aggccataga ctcaactaac
      121 ttagaatatg taaaacttga aagtagcgat ttaagattag aagtatctaa aaaagctcaa
      181 tctacaagtt cacctgtttt aagtgtacaa caagaatcag tagtggatct ttctttagaa
      241 aagccagttg taaacgatac acctgtcaca tctaatgaga atctatcagt agtagtagct
      301 cctttgatgg gaacattcta tgattctcca agtccagatg cagatagctt tgttaaagtt
      361 ggcgatgtag ttgaagaagg cgacacttta tgtatattag aagcaatgaa gcttatgaat
      421 gagataacaa gcgaaattaa aggtgaaata attgaagtat tagttagtaa tgaagaacta
      481 gtagaatata atcaaccttt atttaagata aaaccattgt agtaggaggt atttaagttg
      541 ataaaaaaaa tactagttgc aaatagaggc gatatagcag ttagaatcat cagaacatgt
      601 aaagaactag gtataaaaac tgttgctatc tattctgaaa tagataaaga ttgttttcat
      661 agatatatag ctgatgaatc tatttgtata ggaccaaata atataagtaa gagttataat
      721 aatatagaaa atataatcta tcttgctctt aaattaaagt gtgatgctat tcatccaggc
      781 tttggattct tatcagagaa tcctgaattt gcaaagcaat gtgaagataa taatattatt
      841 tttataggtc caacaagaga acaaatgatt ttgatgggag ataaatctag agcaagagaa
      901 actatgatgg aattgaatat ccctgttgtt cctggttcag aatctgtttt gaaaactaag
      961 gaagaagctt tagaagttgc aagagaaatt ggatatcctg ttatgataaa agcctcaagt
     1021 ggaggcggtg gaaaaggtat gagaatcgtt agaaaagaag aagaactttt ttcaaatttt
     1081 gatatggcaa gttcagaagc tctagcagca ttttctaata gtgatttata tatggaaaag
     1141 tttatagaaa atcctagaca tatagaagta caagtatttg gagataaaca ttcaaatgca
     1201 atacatttag gtgatagaga ttgttctatg caaaggcgaa atcaaaaagt aatagaagaa
     1261 tctttaagtc catacctttc agatgaagaa agacaaaaac ttcataaaat tgcagtagat
     1321 atagtaaaag gagtaggtta tatcggtgca ggtactattg agtttatagt tgacaaagat
     1381 aaaaactttt attttataga gatgaataca agaatacaag ttgaacatcc agttacagag
     1441 atggtaacaa atcttgacct tataaaactt caaatatcta tagctaatgg ggataaaatt
     1501 ccttttaaac aagaagatat tacttttaga ggtcatgcta ttgaatgtag aataaatgca
     1561 gaagattcat caaagaattt tgctccatca ccaggtaaga tagaatcatt aaatttacct
     1621 ggaggttttg gtgttagatt tgatactttt gtatatgcag gatatactat accaccttta
     1681 tatgattcta tgataggtaa gttaatatgt tgggctgaaa ctagagaaga atgtataagt
     1741 agaatatata gagctctaga cgaaattatt gtagaaggta ttaatacaaa tgtagaattt
     1801 caaaaggcac ttgtaacaag tgaggagttt agagaagata ctcatcatac caaatttata
     1861 gaagatgtct ttatgaagaa agaatttgct acattataag gagtaaatag attatgataa
     1921 aaaaattttt atcatcaaaa gaatctaagt atgttacgat ttctttagat gacaacttta
     1981 aaaagaatag tgtagatgat aaattttgga cctattgcaa aggttgtgat agtcatgtat
     2041 ttagaaagga catagaagaa aattcatttg tatgtccaaa atgttcaaga cattatggct
     2101 taagagcaag aaaaagaata aatttattga ttgataaagg aacttttatg gagtttaatt
     2161 ctgatgtaga atttcaaaat ccactaaatt ttcctaaata caaagaaaaa gtagactctt
     2221 ataaggaaaa aacaaaagag tcagaagctg ttgtcacagg ttatggaaga ataaatggaa
     2281 ttaaaacagt tatttgtgta atgaatcctg attttatgat gggaagtatg ggttctattg
     2341 ttggggaaaa aataacttat tcaatagagt atgctgctga aaataattta cctataatta
     2401 tttgttctgc ttcaggagga gctagaatgc aagaaggtat ggtatctctt atgcagatgg
     2461 caaagacttc tcaagcactt tctaagcttg aagaaaaatc attgccatat atatcagttc
     2521 taactgaccc tactactgga ggagtaactg ctagttttgc tatgcttggt gatattataa
     2581 tttctgagcc aaatacgttg ataggttttg caggtccaag agtaatagag caaacaataa
     2641 atcaaaaact tcctgaagga tttcaaactt ctgagttttt acttgaaaaa ggttttattg
     2701 acatgatagt agatagaaga aaaatgaagg aattattata tcaaattctt gctatgcaca
     2761 agaaataggg cataaaggag gaactgtttt gattgaaaat aatagtgaaa aaataaaagt
     2821 cttagaaaat gatattaaac aattaataac tatttctaag gcaaataata tagatttaag
     2881 tgataaaata aattcactta atgagaaatt ggaaaagctt aaggaagatg ctttttcaca
     2941 tctatcagca tatgaaaagg taacactaag tagagatata aagagaccta cgacattgga
     3001 atacatagaa catatttgtt ctaatttttt agaacttcat ggagataggc tttttaaaga
     3061 tgacccttct atggttggag ggattggtca gataggaaag tttaatgtta ctatagttgg
     3121 acaccaaaaa ggaagagata caaaagaaaa tataaaaaga aattttggaa tgcctcatcc
     3181 agaaggatac agaaaagcat taagacttat gaaacaagct gaaaaattta atagacctat
     3241 aataacgttt attgatacat caggagcttt ttgtggttta gaagcagaag aaagaggaca
     3301 aggagaagct attgcaagaa acttacttga aatgagtaaa ctttctgttc cagtaatcac
     3361 ttttgtaatt ggtgaaggtg gtagtggagg agctttaggt atcggagtag gaaatgatgt
     3421 ttgtatgcta gaacattcag tttattctgt gatttctcca gaagggcttt ctagtattct
     3481 atttaaagat tcatctaaag ctaaagaagc ttgtgatgtt atgaagctta caagtaatga
     3541 tttatatgat ttaaaaataa tagataagat tataaaagaa cctcttggag gagcacaaaa
     3601 agatgtagaa gctgtttcaa aagaaataaa agcttatatt ttagaaagat taaatcatta
     3661 caaagatatg gataaagagg aaattatagc tcaaagatat aaaaaattta gaaatattgg
     3721 aaaatgctta tagaatataa agaaatttgt agttttttca taataaaatg gttaaaaaaa
     3781 ataaaggttt tcttaaaacg attatagaag tatattttta taaaagtata ctttaaatga
     3841 tgattgtaat tagtaaatgg caattatgat ttagtacttt aggtaatgga tactttaaca
     3901 aattaacaag gagatgttga aatggaggta ctaaaagttt catcaaaatc taatcctaat
     3961 tctgtagctg gagccttggc tggagtacta agagaacgag gcgttgcaga aatacaagct
     4021 ataggagctg gagctctaaa tcaagcgata aaatcaattg caatagcaag aggttttgtt
     4081 gcacctagtg gaatggattt agtttgcatt cccgcattta ctgatataga aatcgaaggt
     4141 gataagaaaa cagccattaa acttataatt gaacctagat aatatatctc tttattagaa
     4201 tttaattaag ttttaaaggt tggctataga aataggacac tatagtgaac cttttttact
     4261 ttatatgttt tattttatgt aacttttaat gtataatctt aagtaaacaa ataagattgg
     4321 aggaaatata cattatgaaa ttaaaattat tggtagaaga gtatgcagta tgtagattaa
     4381 ataatgacag taaaatacca acatggatag atacagagaa gttttactct ataactagaa
     4441 ctgatgatga attaagtgtt gtatgtttaa ataataatat tccttctgat gttaagtcag
     4501 aaaaggagtg gagaatactt aagatactag gtcctttaga cttttcactt gtaggaattt
     4561 tatctaagtt aagtagtttg ttagcagaca acaaaataag catatttgca atttctacgt
     4621 atgatacaga ctatatctta gtaaaagaaa aagatataga agatgcttgt aaaattttaa
     4681 gctgtaatgg atatgaagta gagtagttaa atacttttac atgaggactt aacttatgat
     4741 ttatataatc gaaattttaa aaataagtgt aataaatatt ttttgtttca taggaatatt
     4801 tattgtattt ggattgattt ttagcattac agaaaatcta aataataaat ttatcttttc
     4861 atcttttgga aaaactggaa ttattgtaac tggagctata ggaacgtttg ttcatgaatt
     4921 gagtcatttg ataatgtgct tgattttcat gcataaaata aattcagtta agtttttcag
     4981 accaatagaa agtaaaaatg atggtatact aggatatgta agtcatagct ataaaaaaga
     5041 taatctttat caaagtatag gaaatttctt tataggaatt gcacctatta taggtggaac
     5101 aataatgatt attttaattt ttaagatatt actgccagat agttatatga aagtgactca
     5161 aaatatagat ttaaagttat atgtaagtat gattaataat ttaaatataa atggttttat
     5221 acacattatt ttaggtgata tttccaattt tataagaata atgttgttaa cacctagtgt
     5281 atattcattg aagtatttta tatttatgtt tttaatgtat tctatatcta cacatatgtc
     5341 acttagtagt gctgatttaa aaaatagctt aaatggtctg agctttatag ttatagttat
     5401 ttttgtaata agttcaatta catacttatt tggatttgat aacttgaata tatccagttt
     5461 aatagttaag tataacatat ttataagttt ctttatggca atagggttta ttt
//
LOCUS       DACTGW010000225         5472 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.227, whole genome shotgun sequence.
ACCESSION   DACTGW010000225 DACTGW010000000
VERSION     DACTGW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.227"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            63..1244
                     /locus_tag="I9573_003148"
     CDS             63..1244
                     /locus_tag="I9573_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAT4797743.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQVDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1386..2303)
                     /locus_tag="I9573_003149"
     CDS             complement(1386..2303)
                     /locus_tag="I9573_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797744.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2556..3350
                     /locus_tag="I9573_003150"
     CDS             2556..3350
                     /locus_tag="I9573_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797745.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3351..5411
                     /locus_tag="I9573_003151"
     CDS             3351..5411
                     /locus_tag="I9573_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAT4797746.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFACYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEELFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2091 a          626 c          907 g         1848 t
ORIGIN      
        1 attagaatat tagaaataat aaataaataa tacattgtac ttaaaagggg gagaaattta
       61 agatgaacaa aaaatatatt aaagcaggga atttaattga tggaacagga aatagtatta
      121 tgcaaaataa gggtataatt attgatggag atactatagt tgaaattgaa gatataagag
      181 atgatttaaa tggatatgat gtgtacgatt acttagataa aactgttatg ccaggcatta
      241 tgaattgtca tgttcacttg acaatggagc cagttggaaa tccaaagata tattatgata
      301 atgtatcaga tgtagagctt gtagtaaata cgataaaaca agttgatgct tatttggatt
      361 caggagtaac atatattaga agcttaggtt gtcccaaata tgtggatgta caacttaaaa
      421 atcttataga caaagggtct atagatggtc ctggtatagt tacttcaggt ccagttatat
      481 gtatgacagg agggcatgga tattattttg gtattgaaag tgatggagtg gatgaatgta
      541 gaaaatcagc cagaacagtt ttaaaaaatg gagtagattg tgtaaagata atggcaactg
      601 gaggtgtaac aactgatgga gttgagccag ggtcaccaca attaactttg gaggaaatga
      661 aggctgctgt agatgaagct ataaaagcag gtaaaataac tgccacacat gcacaaggaa
      721 gaactggtat aaaaaatgca gttcttgcag gaattacttc tatagaacat ggtgtttatt
      781 tagatgatga aataattgat ttgatgcttg aaagaggaac ttacttagtt ccaactgtgg
      841 ctgcaccata ttttattcta aatgcaggaa aagaaagtgg aataactgaa gctactctta
      901 gaaagtgtga gattgttgcc attccacata aagaatcatt cctaaaagca tataaaaaag
      961 gaattaaaat tgcagttggt tctgatgcag gaacttctta taatgaacat ggaaaaacat
     1021 attatgaaat gaagctaatg tcagattatg gtatggataa tatggatata atagttgctg
     1081 ctacaaaaac agcatctgaa cttttaagaa ttgataaaaa ttatggaact ctagaaaaag
     1141 gaaagaaagc agatgttata gttgtaaatg gaaatccagt agagaatata gacgtacttg
     1201 cagatgtgtt agcagtattt aaacttggta aaaaagttag ataaatagta taaaaataaa
     1261 aaagagtgtc taaagaaatt tttaaataat tatagacact cttttttatt agtaataatt
     1321 aaatctttta tactttagaa aatagattct attttatatt tagatataac tctttaaaat
     1381 cattcttatg caatataatc tatagaatct acacatacaa agtatattct tgaatcatta
     1441 gaatttccaa gtactataac atcatttttc ttacctagaa cttcaacacc agttagaacc
     1501 aaactaccag ctactacagt aacttgatta tttatatttg acatataaag attatctagg
     1561 atacctttat tacaacagca atcatcacaa tcgcagttac aattacattc tttgtaatgt
     1621 tttttatcaa gatagtaacc taaagatgct atgaaatctt cataatctgc aggtgtaata
     1681 tcaaatgcaa cagctttaag attacacaat gatgctaaat ctacgtttgc taatggagta
     1741 gatgcagcaa gtataaaatc aattatagca gcaagtattg catctaatat agccccaagg
     1801 tctatcgtag gaggaataac tgcatcaata agtgcaatta attcagctac tcctggtata
     1861 gttcctatag tattaattaa tccctcaaga gttaaaggga ctggaatagg ataagatact
     1921 ctaccagcta tatctattaa atcacagtta caagcagaaa atctttcaaa agtaccatca
     1981 agtccagata aattatcttt tggaggagct gaaagatcta tacctaccaa attagcacct
     2041 actataaaga aatctgagat aaaagcaaat tgattaaagt ttacaaaagg tcttagagca
     2101 tcatatctta gaagttctaa ggcctctctc atactttttt tacaacaaca ttgttttatt
     2161 ttaggttctt tatatttatt tggataatta tagtatctat cagctacttg ataattttca
     2221 tagtatcttt catttgtatt tggataatct tcctcatatt cttgtgtaaa tctaaagtcc
     2281 tctctacatt tattattttc cactaaatac actcctttta tattttaatt taaatgtata
     2341 gataattaag cttatctata tagtaatatt atatttataa tgtaaaaaaa tgttacaaaa
     2401 gatattataa acgaataaaa aataataatt tgtcagataa acttgtaaat ttttatgaat
     2461 aatccattct aaaacaaaaa actgtgtata aaaaaggttt atatgttata tttatataaa
     2521 tataacatat gaacgagaaa ggagtggttt gataaatgat tgaggtagat aaattgtgta
     2581 agtcttttac aagggttgta aaagatgata agaataaaag ttcaataaga aagctaaaga
     2641 aagtaaaaac taaaaaagaa gaattttttg ctgtaaataa tgtttcattt aaagttatgg
     2701 aaggtgaaat tgttggaata ttaggtccaa atggtgcagg aaagactaca ttacttagaa
     2761 tgttaggtgg catattaaca ccaacatcag gaagtataaa tatttctgga tatgattatt
     2821 caatagatag aaattcagca aaaaaagaaa taggttatct ctctggaaat acaaaactat
     2881 atggaaggct ttctccgaga gaacttctaa caacttttgc aagtctatat gagatgtcaa
     2941 aagaagatat agaagaatca attgaaaata ttgtaaaaat aatggatatg agtgaattta
     3001 tagataatag aattgaaaat ctatcaactg gtcagactca aagaacatct atagcccgtt
     3061 gtttaataca ttctccaaaa gtatatattt ttgatgagcc aacactaggt ctggatgttt
     3121 taagtagtgc atctattata gattttatga agaatgaaaa actaagagga aaaactgttc
     3181 tttactcaac tcattatatg gaagaagcag aaactttatg tgacaagatt tttatgattc
     3241 acaatgggaa aataattgca tcaggaacac cacagagttt aaaagaggaa tctggagtaa
     3301 ataatttgag agatgtattc ataaaacttg ccagaaagga ggacatttaa atgagaagta
     3361 agattgtaaa atacatattt aaaaaagaaa tgttagacat attgagagat aaaaagactt
     3421 tatttatgat gattgttgtg ccacttcttt tatatccttt acttatgtta ttaatgattc
     3481 aagtaatgaa tatgtctgca agtagtatga atagtaagga cattaatata gcttttaata
     3541 ataaacctaa taaggtgtta gtatccatga ttgaagatga taatttagaa aaagatacta
     3601 aaaatacttc aaatgataaa ggcaaaataa gagtgttaga tgtagataac tataaaaagt
     3661 cattagaaga aaataaaatt gatgcttata taaagatgga agaaaaaaaa tcatctataa
     3721 attatcaaat atatataaat tcttctcaag aaaattcttc taatgcacta aaaagaattg
     3781 aaactgtttt ggataaatat aaaagattta ccatagaaaa aactttatct gaaaaaggat
     3841 tagatgtaca agctacttta gaaccaataa catatagcac tgtagatgtt gcaaagacgg
     3901 aagaagtggc aggatacttt ttaggtcaga tacttccatt tatattaata ataggagttt
     3961 tactaggttc aatatatcca gcaatagatg ttatggctgg agaaaaggaa agaggtacat
     4021 tagaaacatt atttacactc ccaatatcaa acttagaact ggttatggga aaatatatgg
     4081 cagtttctct tagtgcaata gtaacagcaa ttttaaacat tttatctatg ggactgacta
     4141 tggcatttat tttagttagt ggaggtatta gtagccaatt tggctttgga aactttaatt
     4201 atggagactt agtcttacca attataacta ctttaatttg catatgtctt ttttcaatgg
     4261 tagtttcagc aataagtatg tgtgtatgtt cattagcaaa aagttttaaa gatgctcaaa
     4321 actacataac acctcttatg ttaattatta tgataccatc atatgtttct atgataccaa
     4381 atatagtatt ggatagagtg acagctgtaa tacctgtagt aaatatatct ttacttataa
     4441 aaagtgtatt atcttttaaa agcgatttaa atttaatagc cttagtttta gcatcaaatg
     4501 tagcctttgt tatattggca atcatactac tgtctaagat gttcaattct gaggaaatac
     4561 tttttggtaa tagtaagagt ttttctttct tagagaaaag aagtaatata aaaaaagggt
     4621 ctataccaac tgtaagtgat ggtgttattt tatatgcagt aggattagtt ctattgattt
     4681 atgttggttc tttagttcaa attaaatttg gtatggcagg tattataatg acacaattca
     4741 tgataatttc tttaccatta ttatttgcat gctatattaa agctgatttt aaagaggtat
     4801 ttagcttgac agttcctaaa ttaaagcatg tgtttgggag cattgttctt tggattggtg
     4861 gatacatact tattatgatt ataacacagc taattttatt cttatttcct caaaatatgg
     4921 aagttgctga aagtttaaat aaggctcttt ttatcaaaga tagtgtttta ctgaatttac
     4981 tagcaatagc tttattacca gctatatgtg aagaactgtt ttttagggga tttatatttt
     5041 cttcatttag taaatcaaaa gataaaaata agtctatcaa actcgctata atatgtagtg
     5101 gggtactatt tggtattatg catatggatt ttattagaat aattccaaca tcaatattag
     5161 gtattatatt tgcatacagt gtgtataaat ctggttcaat atttgtatca atgttattgc
     5221 attttttaaa taatagtgta gcagttgtgt taaatcatta tacaactgga aatataacca
     5281 acttatataa atttgttgaa gttgattttt cgaacttaaa tgtacttaaa tttgccttaa
     5341 ttcttctaat aagtcttata ttaattatgt taggattaag agttttagat agaaagtctt
     5401 taagaaatta agtataaagt tattatatag attaaaatat taataagcac taaagtatat
     5461 ggatatatct tg
//
LOCUS       DACTGW010000226         5372 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.228, whole genome shotgun sequence.
ACCESSION   DACTGW010000226 DACTGW010000000
VERSION     DACTGW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.228"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(521..1927)
                     /locus_tag="I9573_003152"
     CDS             complement(521..1927)
                     /locus_tag="I9573_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4797747.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILKDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTIKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGVKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVAGIINAHRGKLDI
                     MSIDSQGLKTILKLPLIK"
     gene            complement(1972..2679)
                     /locus_tag="I9573_003153"
     CDS             complement(1972..2679)
                     /locus_tag="I9573_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4797748.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2850..3764
                     /locus_tag="I9573_003154"
     CDS             2850..3764
                     /locus_tag="I9573_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797749.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVNQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKAKNVVSAQKLLNTKGYMSLIQNGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3764..4525
                     /locus_tag="I9573_003155"
     CDS             3764..4525
                     /locus_tag="I9573_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAT4797750.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPILMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4537..5274
                     /locus_tag="I9573_003156"
     CDS             4537..5274
                     /locus_tag="I9573_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAT4797751.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1854 a          830 c          694 g         1994 t
ORIGIN      
        1 aaaactctac tttagtagaa ctcaatggtg caaaatactt caatttaaaa catttatcgt
       61 actttaaata aataattata ataaaatata cttacaatag ttacatagaa gctattataa
      121 ctacacagaa caacgtataa ttaaaacttt tttaggatgt cattatattt tttcttaaaa
      181 ttaaaaaaca ctataccata attctatttt agtagaattt ttttgtcaat aataaaatta
      241 aatattaact aaaaaataaa aaaaatggaa ttatctaaag aatttaataa ccttacaata
      301 attccaaaat attatttcta tgaaaatttt gataccatga gtaactcagc cataagcgca
      361 taaagcacaa gttactcttt actttaataa aacttgacaa gaaaagaatt tatccttatt
      421 ctactctaat agagttattt tgtcaataaa gaataaaatt attttcaaaa tattttcatt
      481 cttcattctt cattcttatc tgttattatt ttataaaata tcacttaatt agaggtaatt
      541 ttagtattgt ttttagtccc tgactatcaa tactcataat atctaattta cctctatgtg
      601 cattaataat tccagctact atcaaaatac ctagaccatg attttgacct ttggcatatg
      661 cctcacttag tccattttgg ctctttaata aactattaat tctttcttga ctcatacctt
      721 ttccatcatc ttcaacaatt atatagcaaa aatctaaatc agacttagag gtaaccctta
      781 tattacatcc ttgttggtta tgagttatac tattttgtat taaattacta atagcccttt
      841 ttaataattt tttatctgca tctataataa tatcttcatt tagtatatca agttcaacat
      901 aaaagtcatt atccaaatta ttatttataa actcagaaat aagctctctc aaaaacgcag
      961 aaactctaat cttatcacaa ttcaatgctt gcatattata ttcaagttta gatactaaat
     1021 ttaggtcatt tactaattct ctgattttaa ccccttgatt gcatataata gacacttgct
     1081 cttgttctct atttgaaagg ctactgcttt cttcaagttc tccagcatat ccaattatca
     1141 ttgaaagtgg agttcttata tcatgagata ttcctgcaat ccagttagac ctagcttcct
     1201 ctttcttcct aagtttttca tctttcattt gcaactcttt agatacagta ttaatagatt
     1261 ttgaaatatc acttagtatt cctttttctt gtaaaagtac tggttctcct tttgacataa
     1321 gctttatgcc agtaataatt ggttttatag atttcaccat ttttaaacct atgaatagag
     1381 ctattaaaaa agtaactatt gcattaacca gaaacatacc tattattaac ataggaaatt
     1441 gttctatttc cttaatcgta taagtaaggt taaatttagt gtatttgtcc tttggataac
     1501 ctgttactaa tattccattt ggatgttccc atacatacac aggatagtcc tttaaaaaga
     1561 accttgaaaa ctttgctaca tctgtcaaag tataatgact tggaatttct tttggtaaat
     1621 caaatcccca tattctatct ccattgttat caattaacat tgcccaaact ttatttttat
     1681 gtagtacttc cttactagaa tcatctaaaa taaactttcc atcttttaaa atcaaattat
     1741 cttcaatcat aatcaagttt gattttattt ctctgctatc acttgtatct aaattatcag
     1801 agtatttaga tacaaatagc atatatgaaa acagaagaaa acctgctata tttaatacaa
     1861 taaaaagtaa tgaaatcaag atacttgaac ctataaatcg cctcaatatg tactttacac
     1921 tatccaaaat taaatcactc ctcttgattt cttttattct aatatctaaa attaattaat
     1981 catcaactta tatccaagac ctcttactgt aataagatgt ttaggtgtag atggagtttt
     2041 ttctatcttc tctctaaccc ttctaacatg aaccataagg gtattttcat atccatacat
     2101 atcatctccc caaatggctt gacatagtga atcattagta acaattctcc ctttattttc
     2161 atacaacttt aaaagcaacg aatgctcttt agctgttaaa gaaatctctt gcccatcttt
     2221 ctcaacacat gcactattta aattcacaac aacagaatct cccagtttaa atactggcaa
     2281 ctcctcttct tctataggca catatacacg atttaatata cccacaagcc ttaaagtaag
     2341 ttcctttggt aaaaatggct ttactatata atcgtcagcc cctaatccca aaccaatcaa
     2401 tctatcttca tcttcacctc ttgcagagag aaaaataact ggtatcttag atgtctctct
     2461 aatttttctc ataatagaga aaccatctcc atctggcaat acaacatcta aaattgcaca
     2521 atctggcttt acatttctaa ataccctaac agcttcttca taacttgatg ctaaaaatac
     2581 tctactaaat ccctcttttc ttaggatttc atctatcatt tttaataatt ccttgtggtc
     2641 atcaaccaca agtatttttt tatcttttat tgactccata atcatcaccc tcctttagta
     2701 taccatatac ccattttcac aactttgcct aaatagactt ttaaggttat tgtaaggtaa
     2761 acttcagcta actgaaatgt aaccaagtta ttatactgat ataagttaaa agaatcaata
     2821 agcacagatt aaaaaagggg atgaaacata tggaaatgat aattagaaca caaagacttt
     2881 caaaggaata caataaaata tttagagtaa aggatataga cctaagagtt ccacaaggag
     2941 ctgtatatgg ttttttgggt cctaatggag ctggaaaatc taccactctt aaaatgcttc
     3001 ttggacttgc taaacccaca caaggaaata ttaatatttt aggtaaagag ttaaatgaaa
     3061 aaaatagaat atcaatttta aaagatatag gttctttgat agaatctcca tcatactatg
     3121 gtcatcttac agggcttgaa aacatgacca taatgcaaag actcttagat ttacctaaaa
     3181 aaaatgtaaa tgaagcatta aaaattgtaa ggttagaaaa tcaaaaaaat aaaaaagtta
     3241 atcaatattc tttaggtatg aagcaacgtc ttggcattgc tatggcaata atgaagtttc
     3301 ccaaattatt aatacttgat gaacctacaa atggactcga cccagcaggg attgaggaaa
     3361 ttagagaatt aataaaatct ctaccagaaa aatacggcat gactgttcta atctctagcc
     3421 atcttttaag tgaaattgac caaattgcca catcaatagg aataataaat catggcgaac
     3481 tggtttttca agatagcata aaagaacttc ataatagagg tcaaagtcag attgctatca
     3541 aagctaagaa tgttgtatct gcacaaaagc tattaaatac taaaggatat atgtcactta
     3601 tacaaaatgg gtacttgact attgaaaatt taaaagactc agatgtggct aaaataaatg
     3661 gtatgcttgt ccattcaaat atagatgtat ttagaataga agaacataaa aaaagcttgg
     3721 aagatatatt cctagaactt actggtaagg cggtgacttt ataatgaaat accttggtct
     3781 agaattttat aaacttaaaa gaaagaagat tattctgatg acctttttat ttgtagctgt
     3841 tgaaataatg tggtgtatcg taaattcaaa tagggctatt acacgtaatc cagatatgtt
     3901 aggtggattt gaatattcct atatgcttat gagttttgca agtttaaatg gtctattctt
     3961 tcctatctta atatcaataa ttacatcacg cataagcgat atagaacata aaggagatac
     4021 atggaagctt ttaaaatctt ctgtgacatc tttaaatagt atttatttat ccaaattttt
     4081 atgttctgct attttggtat ctatacctat attgatgcaa gtgttatcta ttgtgttgtt
     4141 tggacatttt agaggggtta ttgagtcaat ttctgtatct ttacttttaa aatacacatt
     4201 aggaacaatt cttgtaagta ttgctatcat tgcactacac ctatggattg caactgtaat
     4261 tccaaatcaa atgattgcaa ttacatttgg catgattggt agttttatag gtttgacatc
     4321 aggtttgttt tatcaaggta ttcgtagatt gtttatatgg tcttattacc tagagttatc
     4381 acctttaagt tatgcttatg ataatgtttt aggttcatct gtctatagaa ttgatatgaa
     4441 tttttctatt acgattttag ttttcttaat tggaatgcta atttattata ttggaagaaa
     4501 tcatttatta aaaaaagaag tataggaggg atttaaatgt taaggaagtc agttttggga
     4561 gaaattctaa agttaaaaaa ttcttttata tggtatgtat tattagcttt gccacttata
     4621 agtgtgttga ttggttctgg taatttttat ctaaatcaag gcatactcaa aaaagaatgg
     4681 tacagcttat ggactcaagt tagtcttttt tatggggaat ttttctttcc tatcttaatc
     4741 gcaatctttt gtgcatatat ttgtaggctt gaacatatga atcataattg gaataatgta
     4801 atgacactgc cagttaattt aaaaaatatc tttttatcta aacttacagt agtttcaata
     4861 cttacagcta ttacacaagt tttctttgtt atcttttata tagttgcagg aaatatgttt
     4921 ggtttttctg gtagtgtacc taaagaattg actggttgga ttgctaaagg ttggattatt
     4981 tctatttcaa tagcttctat acaattatac atgtctataa agattagaag ttttgcaact
     5041 cctattggta ttagtctttg cctttctcta tttggtatgg gattaatagt agcaaaacct
     5101 gctgttggta tatttttccc ttattctatg ttaggtagtg ctatgggggt tataaagcaa
     5161 tctgctttaa atgttaatga cactatagtt acaatatgtg ttgcatttat tactactata
     5221 gtattttctg aaattgccaa tagaaatttt agaaagaatg atgttatttc ttaatggtaa
     5281 atgctagata ttttttggtt tgaaatttat attctttttg gtttgaaatt tatatccctt
     5341 ttatttaaaa atatatatct tttatttgaa tt
//
LOCUS       DACTGW010000227         5330 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.229, whole genome shotgun sequence.
ACCESSION   DACTGW010000227 DACTGW010000000
VERSION     DACTGW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.229"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            160..996
                     /locus_tag="I9573_003157"
     CDS             160..996
                     /locus_tag="I9573_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAT4797752.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFKFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1349..2926
                     /locus_tag="I9573_003158"
     CDS             1349..2926
                     /locus_tag="I9573_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAT4797753.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2919..4103
                     /locus_tag="I9573_003159"
     CDS             2919..4103
                     /locus_tag="I9573_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAT4797754.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     TL"
     gene            4121..4720
                     /locus_tag="I9573_003160"
     CDS             4121..4720
                     /locus_tag="I9573_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAT4797755.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4744..5214
                     /locus_tag="I9573_003161"
     CDS             4744..5214
                     /locus_tag="I9573_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAT4797756.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIELKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         1994 a          564 c         1026 g         1746 t
ORIGIN      
        1 gacaaattaa ttgatgacaa taaaaaatat tttgttattg tagtattata gggtaaattg
       61 caaaatatat acgaaagtaa aattttaaat attgacatta aattaaatat actatactat
      121 gtaagtgtga tgagagaatt tttttagagg ggatttatag tgaatataaa aaagatattt
      181 aataacaatg tagtagtaag ctccctagaa gatggcacag aaataattgt cactggtgct
      241 ggagtaggat ttaagaaaaa agtaggcgat ttgatagatg aaaatttgat ttctaagaaa
      301 tattttgttc aagacgatca aagggacaaa tacaatcaaa tacttaataa gacctctatt
      361 gaatacttta aaatttcaga agaaattata gaaaaggcta atgaagtttt aaatactcaa
      421 gtgaatgatt caataatatt agccttaact agtcacatag aatttgctgt ccaaagagaa
      481 aaacaaggta taaaattacc aaacttaata ttgaatgaga caaaacaact ttatagggaa
      541 gaatttaaat ttggattatg ggcaattgat gaaattgaaa aaaggatagg aataaaattg
      601 cctgaagatg aagcaggata tattgctatt cacataatca atggtttgga aaattctcaa
      661 aaagatgaag gtattaatat actagagttt tcaaaacaag ttattcagat aattgaagaa
      721 gtacatggat ttaaattgga tgtaaattca ttaaattata ctagacttat aactcactta
      781 aagttttttg ttcaaaggat tctccgaaaa gaaacttaca atgatagtga tattgaagat
      841 atgtataagt tagtgaataa aaattacaat aaacctaaag tgtgtactga agaaattgca
      901 gcattggtat tagacagatt tgaatataag attagtaaag aagaagaatt atatttaatg
      961 attcacataa ataaaataac aaacagtggt tgttaggatt gtaactgata aagcaggcaa
     1021 aacctaagtc aatgagctta actattagat taagtttttt gatttaggtt tttttattag
     1081 atacaatcaa aatacagaca gtgactttta ggattgtaac tggttaagca ggcaaaacct
     1141 aaatcattga atttaactat taggttaagt tttttgattt aggttttttt attagataca
     1201 atcaaataca gacagtgact tttaggattg taactggttg agcaggcaaa acctaaatca
     1261 ttaaacttaa ttattaaatt aagttttttg atttaggttt ttctataaat ttgattatgt
     1321 ttttagataa aaataggagg ggaaaattat gaaaaaaaat aagttaggtt tatggaactt
     1381 cttccaaatg ttagggaaaa cgtttatgtt cccaatagca ctacttagtg tttctgggat
     1441 gatgctagga cttggggcag gttttacaga ccctaagatg attgaaatgg ttccatttct
     1501 agggaatgaa tatgttaact tagttttaaa cttcatgtta acaataggat tatttgcatt
     1561 taacaacttg ggagcattat ttgcaatggc aattccacta ggattattaa aaaaagaaaa
     1621 agagtttggt gctttttcag gattagtagg atttatagca atgcatatag gtacaaattt
     1681 ttatttaaca agagctgatt tgttagtacc agcagaacag atgtcaacta atggtcaagc
     1741 tatgattatg ggtatacaaa catataatac aagtgtttta ggtggtattg tagcaggatt
     1801 aattgtttat gcaatatatg ataaagttag taatttaaag ataccagaat cattaggatt
     1861 ctacagtgga ccaagattag ttccaattgt tagtttaata gttatgagta tagttggatt
     1921 attaatacct attgtttggc caccattttt caatgcattc caaagtttag gaagatggat
     1981 ttcttcagca ggaccattag gatatttttc atatgcagtg gcagaaagag ttacaattcc
     2041 atttggacta aatcatcttg taacatctgt attccgtttt acaccaatag gtggaactgc
     2101 tgttattgat ggagaaacgt actttggtac attgaacatg tttatggcat atgtagaaaa
     2161 taatcagatt atacctcttg atttagctgg taaaatggaa caaggtaaat tgatgattca
     2221 atatggatta gctggtgctg cattggctat ctaccgtaca gcaaaaccag aaaatagaaa
     2281 ggcacttaaa ggacttttaa tatcaggagt gttgacagtt attataggtg gtataagtga
     2341 accaatagag tttctattct tattcatatc accagcatta tttgctttcc atacatttat
     2401 gaatggtcta gcaaacatgg tacttccata cttaggcgtg ttgatgggat tcacaggaga
     2461 tttaattgca tttataagtt ttggtgtgtt aagaggaaca gctacaggat ggccaatagc
     2521 agtattagta gcagttttat attttgcaat atactatttt gtatttaagt gggcaattat
     2581 taagtttaat ataaagaccc caggacgtga ggataaagaa ataaaagtat ctgaaggaaa
     2641 tggttcagat ttcaaaaatt tgtctacata taaaggacaa caaatgatat ctgctttagg
     2701 tggtcaatct aatatattat cactagataa ttgtgtaact agattgcgtt taaaactaca
     2761 agatgtatca ttaataaatg aggatgcaat taaagaggct ggtggtatag cagttgttaa
     2821 attggatgag catacaatac aagtaattat aggaactcaa gtgtattctt taagaaagca
     2881 gatggataaa gctatggaag gtcatttgga ggcatgtaat gattaatttt gataaaaaag
     2941 tagaccgatt gggtacatat tgtacccagt gggactatgt agaagataga tttggtaaga
     3001 aggatttgct tccttttaca atttcagata tggatttaga gtttccattt gaaataacag
     3061 aggtgttgca ggaaaggtta aatcatagag ttttgggata cagtagatgg aaacatgatg
     3121 attttagaaa ttcagttacg aattggtata ttaaaagatt taattgtagc gttgatagta
     3181 actggatata ttatagtcca agtgtaatgt attcaattag caaacttatt gagatgttaa
     3241 gtaatgaagg agatggaatc cttataaata ctcctgcata caatgctttt tatgatgtga
     3301 taacaaataa caatcgtaat attataaagt cacatcttgt aaataggaat ggattctatt
     3361 caattgattt taatgatttt gagaaaaagt gtaaaaaatc taagatattt attttatgta
     3421 atcctcacaa tccaactggt agagtatgga gtgagttaga acttgttaag atgataggaa
     3481 tttgtaagaa taatgatgtc aagataatat cagatgatat acacatggat attgtatatg
     3541 acaatagaat gacacctatc ttaaatgttg caaaggacta tttagattct gtattcattt
     3601 gtacatcagc gtctaaatct ttcaatatac cagcattgac tgggtcatat gtgattatac
     3661 caaatgaagg tattagaaat aaatttgaaa atatcactag ataccgtgat tttgtaaatt
     3721 cgccagcaat tctagcaata ttagctacaa tggtctgtta tgatgactgc gaatattggt
     3781 tagatgaact attaaaatac cttaaagaga atcttaaata tacgattgat tatataaaag
     3841 ataatttaga accattgaaa ttggtcatgc cagaaggctg ttactttgct tggatagatt
     3901 tttccgaact taaaataagt ggtgagaaat tccaaaaatt attgatggat attggagaag
     3961 ttgctattat gtcggggtct gtatatggtg aagaaggtga gttttattta aggttaaatg
     4021 tggcatgttc aagagaaaaa cttgaagatg gtttaaatag gattaaaaaa actatggatt
     4081 atattgcaaa aagaacttta tagtaggaag ggagatagtt atgggatatt taaaaacaat
     4141 ggttgatgtt atgactactg aaatgaatgc cataagatgt ttaatagatg aagcaggaat
     4201 agaatatgaa agtatagtta gtgaaattgc aaattgtgaa ggcaaagtaa tttttatggg
     4261 tgtaggaaaa tctgcacata taggtaagaa attggcagct acatttgcta gtactggtac
     4321 acctagcttt tttgtacatg caacagaggc agtgcatgga gatttaggaa tgattgaaag
     4381 taaggatatt acaattttaa tatcaaatag tggaaattct atggaagtgg taaactgtat
     4441 aaaatatatt aaagcaatag gttctaagac aattgcattt acttcaaaca gaaattcagt
     4501 actggcaaaa gagtgtgatt atgctttaat atatcctgca aaggatgaag cagaccattt
     4561 aaatctagca ccaacaacat catctacaat tactttggtt ttaggagatt ctattgcatg
     4621 tgcattatct aagagtagta attttggttc ttcagatttt tataagtacc atccaggagg
     4681 aagtttaggt gagaagttaa aaacagctaa caatggataa aagcaaaaag gagagtagtt
     4741 tttatgttca aaatgtttaa aaaaaaatca ataaaatcac ccatatcagg aaaagtaata
     4801 gagttatcta aaatacaaga tggtgtattt tctcaaaaaa ttatgggaga gggtgtagct
     4861 atagattcta caggagacat tgtatatgct ccaatcaatg gaaagatagt agtagtagca
     4921 gagactaaac atgcatttgt aatagaatta gaaaatggga tggaacttct tattcatgtt
     4981 ggtttagata ctgtaaattt aaaaggcgaa ggttttgagg cattggtgag tatgggagaa
     5041 caagtgaagg aaggaacacc aatgttgaaa attaaccgtc ctttgattga attaaagggt
     5101 atatcactta ttactccagt tacaataact aatcattcag agtacaatat gaatacatgt
     5161 aatgtaggca atagcgttga aggtgggaaa gatactgtaa ttgagtttaa ataaagaatt
     5221 aaaatacaag gttaatattt ataaatacct ataaacttat gttttataag attgattgta
     5281 tggatttatt gtataaaaaa gaactcttta attataaatt aaggagtttt
//
LOCUS       DACTGW010000228         5299 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.230, whole genome shotgun sequence.
ACCESSION   DACTGW010000228 DACTGW010000000
VERSION     DACTGW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.230"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            101..1492
                     /locus_tag="I9573_003162"
     CDS             101..1492
                     /locus_tag="I9573_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAT4797757.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKEDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            1607..3076
                     /locus_tag="I9573_003163"
     CDS             1607..3076
                     /locus_tag="I9573_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT4797758.1"
                     /translation="MSNVNKKLVIAIGAVALVAIIFIGIMCMQFKGEKIAKNTYVNGV
                     DIGKLTKSQAKQELAKKYKLENVEFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SSFFGNLSKTISTNFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLTNIDTNLGTY
                     STIFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            3141..3734
                     /locus_tag="I9573_003164"
     CDS             3141..3734
                     /locus_tag="I9573_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAT4797759.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            3871..5262
                     /locus_tag="I9573_003165"
     CDS             3871..5262
                     /locus_tag="I9573_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAT4797760.1"
                     /translation="MSIILVKDIKQDIESMPRKILDISLIEDNKSDLTFFNIEEEDGD
                     ETLKALKQSYVTTNYKEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYI
                     TSAINMVNKALEINPKDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVN
                     LISSEDFKTFLGRYNHSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLG
                     NVKKREGYLDKLKELDKDNPVFEKNEEEKEDTSKKEEIRKVKRKKSILPYIIVGCVLI
                     GMGIYLIQSKKRIENLNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPER
                     EITVADEEDLYYQALNLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLG
                     NKDEAIKYYKKYINTYTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSM
                     YNNSKIKEILSEK"
BASE COUNT         2353 a          471 c          914 g         1561 t
ORIGIN      
        1 cttgtgtttt tatttttttt caagaattat gatataataa ccaataagaa atttagttta
       61 ccataaaata taaagattat taaatggaga gggaactata atgaatttta gagatttatt
      121 tgagaaggct gtagatttta ttgatgaaaa aaggaaatct atagttgcga tttcattatc
      181 aacattaggt gtaattgctt tgataatagt atttttctta agtagtaacg agtttagtgt
      241 aggaaatgaa gctaatgagt tgttaaagat tatagagaaa agacagtatt ctatagctgt
      301 tgattactac acaagtatag aaaaaaagtt ttctgattca aaaatggaaa ggtttaataa
      361 gtctgtttct aaaaaaataa ataaattgct attaaatagt ggtgataaat atttagatgg
      421 agacataagt caagaaagtt ttattggatt aataaatact gtgaaagaat taaacaaaat
      481 aagtatagat gtggatgact tattagctca agctgataga gttagagaaa tgtataaaga
      541 agaaaatact acttatgata ttgccattaa ctacataagt actgtttcaa tattaaatgg
      601 tatgggtagt aacctagatg tatacaaaca gaatatagaa acaatcaaag agtctagaga
      661 tgtttatgac tcagcagtaa aagaccaaaa agcatataag tattatgaag ctattgaaaa
      721 gtataataaa gtattaaaag aagatgaaaa gtattacagt atggctcaaa atgggaaaga
      781 acaatgtatt gaagaaatgt atgattacta tataagtaga gctgaagagg caaatacttc
      841 tggagattat gaaagagcat tacaatatat tgagtattta aaagaagatt attcagatga
      901 tgaaaaggta cagagtttag aaaaaaaata taaaaagaat ttatcattgt ataccttaac
      961 atctgatgac attataaatg taatagctaa aaaaagtggc aaagacaagg caaatctatc
     1021 tataaattca cttccacaaa tgataaaaaa taataaatac tattatgctg aagtatatga
     1081 gtatgataaa ttaataaatg aagttttggt aagtgcaaaa actaaagatt tatattcgta
     1141 taaagatggt aaaaaagatt acaaagtaga ttatggtgat ggatacttta gaatattaga
     1201 agatggaagt tatcaatttg gtattacaaa agataaagct aagtttgtgt taactaatac
     1261 tcttgatgaa aaagaaaata agtataaaaa gattgagata ttagatatag agaaagcaga
     1321 tagatatgta aaaagtaaaa agagtttaga agaattgttt ggaaaatata aaaatattta
     1381 ttattatgct gtagtaaata aaggcttatt tagaggcaaa gaagtttatg ctattaatat
     1441 ttacaatgaa aaaatatatg ccatatcaga aaatggacta aatgaatatt aattaataat
     1501 attaaattaa tatataatta aaaagttttt ggggttttaa ggcgcattat gtagtaatat
     1561 aataataggg atatataaat aattttattg taaggagaag tatgggatga gtaatgtgaa
     1621 caaaaagcta gttatagcta ttggagctgt agcattagta gcaataattt ttataggcat
     1681 aatgtgcatg caatttaaag gggagaaaat agcaaaaaac acatatgtta atggagtaga
     1741 tattggaaaa ttaactaaat cgcaagcaaa acaggaatta gctaaaaaat acaaattaga
     1801 gaatgttgag tttaattaca atgataaaag ttggaaagta aagagcaaag atttaaattt
     1861 atctactgat ctggataaaa cagtagaaaa cgcatataat ttaaatagaa agagtagttt
     1921 ctttggcaat ctatcaaaaa ctatttcaac aaattttggc aaaaaaagca acttagtagt
     1981 tgtgataaat tatgataaaa ataaattaaa agcagaaatg gaaaaaatag ctaaagaaat
     2041 agatgtagat gtaaaagatg ctacacttga tataagtggt gaaaaagtca aggttatacc
     2101 agattctgat ggtcttaaaa tggatatttc taagagtatg gagaattttg ataatcaaac
     2161 taaaaaaggt aactataaaa atgagttagt agtaaaagct actccagcaa aagtaaaaaa
     2221 agagcaactt actaatatag atacaaattt aggtacgtat tcaacaatat ttaaaacttc
     2281 tcaaataaat agaagtataa acataaaatt agcaacagat aatataagta atgtattact
     2341 tatgccagga gaaactttct catttaataa acatacaggg aaaagaagta aggaaaatgg
     2401 atataaaagt gcacctgtaa taatggaagg tgagatggaa gaagattatg gtggaggagt
     2461 ttgtcaagtt tcatctacgt tatacaattc tgtgttatac gcaggtttag aaattgttaa
     2521 tgtaaaaaat catactatac catcaagtta tgttccaaaa ggaagagatg caactgttgc
     2581 agatagtgga atagatttct tatttaaaaa taatctaaag catccagttt acataaaaaa
     2641 ttacgtttca ggaaatcaaa ttgtttgcaa tatatatgga agtgcagaag ataaacaaaa
     2701 tataacgata tcaacaaaat tagatggcgt ttcacaaact acaatgaaaa gagttaatga
     2761 ccctacaatg cctaaaggta aagaaaaagt agacaaaagt ggaagaaatg catattctgt
     2821 atcaacatat agaacattta atgatgcaaa tggtaaaaaa attaaaactg aaaaaatagc
     2881 taattcatat tatcctaaga aggaaggtat tatattagtt gggactatgg aaccaaaacc
     2941 agaggaaaaa ccaaatacag acgaaaataa gaataatcaa aatacaaaca atcaaaatcc
     3001 aaataatcaa caaaaaccag aaacaccacc aactgataat aagcctaatg agacacaacc
     3061 acaaccgcaa gcttaaagtt ctcaagaata ggatttatgt agagtaataa aaaatataaa
     3121 tctaaatagg agggattact ttgaatttaa ataatataac gaattacgtt caaagtattt
     3181 gcgagaatat ttctaatgta ttggatgtag atgttacttt agttacaaaa gatttaacta
     3241 ggatagcagg tacaggaata ttcagaggta aaataggaga gacaatatca gagaagtcag
     3301 tgtactctct tgttttaaaa gatggaaaat cctatgttat aaataaggaa atagaagaga
     3361 actgtaataa atgtgcaaat agagaaaatt gtaaagaatt agctgatata tgtactcctg
     3421 ttaaaatggg agaacatata cttggaatct tgggtatagc tgcgtttaat gaagagcaaa
     3481 aagaaaagat actttcaaaa gataaggagt taactgcttt tgtaaggagt atgtctgatt
     3541 taatatcttt taaactagat gaactttata aacaagaagt taagactata gatgaccttg
     3601 agagggaagc tatagaaaat gccataaaga aatatggaag taatacagaa ggtatgaaaa
     3661 aagtagctga ggcattaaat ataggtatag ctacactcta tagaaaagta aaaaaattca
     3721 acattaaaag ttaatagtat tgaaaaagag gtaacctttt tatgaaaaga agaggattac
     3781 cttttttatt atgagaatgg tggaaaaata tgtatagttt tgacttgaca tattaaatat
     3841 gttatcttaa atatatattt tagagtggag gtgagtatta ttttagttaa agatataaaa
     3901 caagacatag aaagcatgcc tagaaaaatt ttagatatat ctttgataga agataataaa
     3961 agtgatttga ctttttttaa tatagaagaa gaagacggtg atgaaacatt aaaagcatta
     4021 aaacagagtt atgtgactac aaattataaa gaagaaactc tattaaactt gcaaggagct
     4081 ttaaattcaa agtcagacac aacgtctaag gagttaaaat tagaatcata taaaagatat
     4141 aacaatgcat tggatttagc atataaaaat tatataacta gtgccattaa tatggttaat
     4201 aaagctctgg agataaatcc taaagatatt gatatactaa atcttagagg tcttttaaag
     4261 ttattaaaat gtgattttgc taaatcattt gagagttttt atactgcttt atgttatgag
     4321 aataatcata tatctagaaa atatgtaaat ctaatttcat ctgaagattt taaaactttt
     4381 ttaggaagat acaatcattc tataagattt ataaatgaag aattaaatta tgagtcaata
     4441 catattttag agaacataat tgaagaagag cctgagctaa tagagccata tgtaatctta
     4501 tctttgttat atgataaact aggtaatgta aaaaagagag aaggatattt agacaaactt
     4561 aaagagctag ataaagataa tcctgtattt gaaaaaaatg aagaagaaaa agaagataca
     4621 agtaaaaagg aagaaataag aaaagtaaaa agaaagaaaa gtattttacc atatattata
     4681 gttggctgtg ttttaatagg tatggggata tatttgattc aaagcaaaaa gagaatagaa
     4741 aacttgaata accaaataag tagtaaagag gaaaagttaa gtgaaactga taaaaagtta
     4801 ggagaaacga gtaaaaaatt agaaaaaacg aataaggaat taaatgaggt taaaaataaa
     4861 gaaccagaaa gagagataac tgtagctgat gaggaagatt tatattatca agctctaaat
     4921 ttaaagaaaa acaaagaata tgaaaaagcc atagataatt ttaaaagtgt agtttcttct
     4981 ggtaaaacta aaaaatatat ttctgagtca atatatcaat tagcaataac aaataaatta
     5041 ttaggaaata aggatgaagc tataaaatat tacaaaaaat atataaatac ttatactaaa
     5101 aatgaccagt attatgatga ttcatattac gagttgggta tgctttatta tgataatggc
     5161 gatttaaaaa atgcacaaca gactttttat agtttgagaa gtgaagttcc agacagtatg
     5221 tataacaatt caaaaataaa agaaatctta agtgaaaaat aggtttatat agttaagttt
     5281 tttgaaacaa ccaagtatg
//
LOCUS       DACTGW010000229         5296 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.231, whole genome shotgun sequence.
ACCESSION   DACTGW010000229 DACTGW010000000
VERSION     DACTGW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.231"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            114..1178
                     /locus_tag="I9573_003166"
     CDS             114..1178
                     /locus_tag="I9573_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797761.1"
                     /translation="MFLKLIFKYNTLDFSRAYFDLTDWADNPKDISYYLSNGTFGDLM
                     DSIDENASKMSLSDIAKLEKKIAKKHINLWNEGKIDFSTTRFSSIVPMLQNAGVNFHF
                     IYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYITVKDTNYDDSTLDQVYKILNSIKK
                     DKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTACYIKESIENSTNIKVCVGYGIGNDI
                     KQAMSSAISANKESKINPLNYSFLVNENYETIGPLLCDKLLTVSNKVTPHINTIAKSS
                     KLSTLTIQKIISVTEILQRDEVTPKELSSCLNVTVRTANRFLSSLLKSGNATILYEKQ
                     NSTKGRPERVYKLFISENKY"
     gene            complement(1306..2496)
                     /locus_tag="I9573_003167"
     CDS             complement(1306..2496)
                     /locus_tag="I9573_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4797762.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEESTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEIIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(2602..3981)
                     /locus_tag="I9573_003168"
     CDS             complement(2602..3981)
                     /locus_tag="I9573_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4797763.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELSAY
                     GHGCGHNLLCTAHVGAVVGLKREMLENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(4012..5220)
                     /locus_tag="I9573_003169"
     CDS             complement(4012..5220)
                     /locus_tag="I9573_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797764.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNVLPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFRLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT         1777 a          982 c          659 g         1878 t
ORIGIN      
        1 ctgtgaagaa ttagtaagac atattttagt atgcaaaatt ctatgaagca ggaaccctgt
       61 gactttagtc atgggaggtt cagtttggta ctgacttaca aagttactat gatatgtttc
      121 taaagcttat ctttaaatat aatacattag atttttctag agcatacttt gacttaacag
      181 actgggcaga taatccaaaa gatatatcat attatttatc aaatggcaca tttggagact
      241 taatggatag tattgatgaa aatgcatcta aaatgagttt atctgacata gcaaaacttg
      301 aaaaaaagat tgcaaaaaaa catattaatc tatggaatga agggaaaatc gatttttcta
      361 ctactcgttt tagtagtatt gttcctatgt tacaaaatgc aggagtaaac tttcatttca
      421 tataccctga tatagattta ttaaaaaata cttttaataa cttaataaaa gatattcatt
      481 tacatagaat gaagggaaat caaccaagtg taatatatat aacagttaaa gatacaaatt
      541 atgatgactc tactctagac caagtttata aaatattaaa tagtattaaa aaagataaat
      601 taaccgattt tataattgaa aaaaatagta attcttttaa aattttcact aaatgtgaaa
      661 cgattaaatc attaactaaa aattttactg catgttatat aaaagaatct attgaaaata
      721 gtacaaatat taaagtatgt gttggatatg gaattggtaa tgatataaag caagctatgt
      781 ctagtgccat aagtgctaat aaggaatcta aaataaatcc tttaaattac agtttcttag
      841 tcaacgaaaa ttatgagaca ataggacctt tactgtgtga taagttactt actgtttcaa
      901 ataaagtaac tccacatata aatacaattg ccaaaagttc taaattgtct actcttacta
      961 ttcaaaagat tatatctgtt actgaaattc tacaaagaga tgaagttact cctaaggaat
     1021 tatcttcatg tttaaatgta acagttagaa ctgctaatag atttctaagt agtctcttaa
     1081 agtctggaaa cgctactata ttatatgaaa aacaaaactc aacaaaaggt agacctgaaa
     1141 gggtatacaa attatttata tcagaaaata aatattaaaa tccaaaatgc ataaaaatat
     1201 tttatcatct aatattaagt aaaattatat atgactttct aaaaggtata tctttgcatc
     1261 tttttaagat atacctttta tgatatttaa taatatttta tgttattatc tactaaaatt
     1321 attttctaac cattttactg cacaatgagc aaataaagct gaaccataaa ctaatccctc
     1381 ttcattaaaa attgctttag gattatgcat accaaaatta tatccctgac taatacttcc
     1441 cattccaatc cataaaaata ctcctggaac ccttagtaaa atctctgaaa aatcttctga
     1501 acccattgca gatggaaagt ctattacatt ctcatttcct ataatttctt taacataaga
     1561 tacaatctca tctcctacat ccaaatcatt tataactgcg ggagtcccca ttttaaattc
     1621 cactttagca tttcctccaa atgatttagc tattcctgta gatatttcaa taattctatt
     1681 ttttaatttt tctctaacaa attggtcaaa tgttcttaaa gttcctgaca tagatgcttt
     1741 atttggtata gcatttggta cgtttcctgc attaaacata ccaatagtta aaactacagg
     1801 ctctttctga ggtttttctc ttgaaagtat agtgtttaat gctatttgta tatgcgacat
     1861 tatgtttatt gggtccactc caaattcagg gcttgctcca tgagttgctt ttccttctac
     1921 tgttatttca aatatatccg cagatgccat agctggacca cgtgaataag ctacttgacc
     1981 tgtgttaaga aatttaccca ccatactgtg aaatgccata gctgcatcga cctgaggatt
     2041 ttctaatata ccatcatcaa tcattgattt tgcacctctg gtactctctt catctggttc
     2101 aaatacaaac tttactctac ctttaagtaa gtgcttatta tccataagta ttttagctgc
     2161 tcctaagagc attgctgtgt gattatcgtg tccacaacta tgagatgcat ctttattctt
     2221 acttttaaaa ggtaaatccg tctcctccat aataggaagt gcatccatat ctgcccttaa
     2281 taataaggtc tttccaccat tttcactgtc taaaacagct actattccat tattagacgt
     2341 caatttcgga gaatatccca ttttttctaa agtactggta acaaaggtta ctgttttcgg
     2401 tagattgcaa ccaatttcgg gattttcatg taaatatctt ctccatctaa taatttcccc
     2461 ttctattgac tgcgcttctt taaaaaaatt tttcatattc cttcctccta atcagatagt
     2521 cagcaaaatc tcactttctt ttagtaatga aatgatgctg atataaaaat tataatttta
     2581 tatattaaac ttcatgtctt tttaaggagt tggaatatca tctggaatag gacatttata
     2641 tgttttacca ttcatagatt tatcaaactc ttcttttgcc tttttagcta tctctggatt
     2701 atttaatatt ttggctccaa acatagccat gatttttgat gcatatatca tacctttttc
     2761 tcctatagaa cttcctgcac aagatgtaaa ctgccagcta tgacctggtg cacctagatt
     2821 cgtacaccct gtaaaaaagt atgctgtcgg aactatatgc attacatctc caatatcagt
     2881 agaaccataa ctattaaagt ttgtaacatt tcctccacca taatatagat gagttccttc
     2941 tggcaaatta tatttactta tcatagcctt atattgttgt gggctttgtc tatctaattc
     3001 agctgcaaaa tccaattcct ctcgagtcca aggctcttgt ttaatctcat tcatacattc
     3061 tgatacaaga tttgctaaaa catgattatt taaagtatta taacaacctc ctaaaaactc
     3121 tacttcaact tcagtttcag tcatcattgc agctcctttt gctaccttta caagtctttc
     3181 atatgtactt tctacagctt ctctacttaa agctcttgta taataccata ctgatgcttt
     3241 atcaggaact atgttgggtg ctactcctcc atctgtaatt gtataatgaa tccttacatc
     3301 agaagtaaca tgttctctca aataattagc tcccacatta gtaagttcca cagcatctag
     3361 tgcacttctt ccattttgag ggtctgagcc tgcatgtgca gtctttccct taaagtgaaa
     3421 ttttacagag ttaacagctg ttgaagtacc aattgtagct tcactaattt tatttggatg
     3481 aaaatttata gctaagtcta acccttcaaa tgctccacct ctagccataa atcctttacc
     3541 tgttaattgt tcttctcctg gacatgcata aaatactata gtcccactta agttattttc
     3601 tagcatctct cttttaagtc ctactactgc ccctacatgt gctgtacata ataaattatg
     3661 cccacatcca tgaccatatg cactcaattc aaatgcttct tgctttgttg aaactttttg
     3721 atttaaacca ggtaatgcat caaattctcc cataaaacct ataactggtt tacctgaacc
     3781 aaaactagct ctaatagccg tggctaaacc tcctacacct acttccacat caaatccttc
     3841 acttcttaga aagtttgctg tattttcaca tgctttaaat tctttaaatg ctttttctgg
     3901 gttttcccat attttcttac ttaaatcatc aagtttgaat ctttttttat ctatctcatt
     3961 tatagatata cttcctatca tttcttattc ctcctataag tatatttact atcaaccaaa
     4021 tataattgga cctattatac tagcaaatgc cactgatgca atggttacag taacaaatcc
     4081 tcctataatc atctttggta ataaataatc tgttgccctt tgtttttctt cttcagttcc
     4141 ttccatagca tcaatacctt cgttggaaat tatctgtgtt gcaggataag ctagcataca
     4201 agttacacca attgcagctg acattcttgg agaatatccc aatagttttc ctacaattgc
     4261 agtaaataat attataaata cagaacctac aacaagtatt cctatagttg gaacaatcat
     4321 cccaaacaat gctgttggag ttaccttagc aagtcctcca ggtaacatca acatacaacc
     4381 aaacattata attccattag attgagattt atctaaaatt cctttttcta aaaaacctat
     4441 tcgtgtaaaa atcaacccta ctaacaaaca tgctatatta ggatttaaat aatagtttgc
     4501 aggggtaaat cctggtatta aagtagccaa tcctacaaaa ttaccaatca tagcaacaaa
     4561 tgaaagcttt gcaagataca ctagagtaga tgatttttta cctaaatctt ctttaaaaat
     4621 tctcatactt ggaagtctaa aattactttt tgactcatct ttcaaaaaat ccccgttatc
     4681 tcttttaaat tttaattctt tatttataca atatgtagtt atcggaatgc caaagaactt
     4741 ttgaaatgat aatactagaa ttacaaatgc agcaagttca ggtctatttg cagcttctgc
     4801 aacagttgtt acgataatac cggcaacagt agaacctgct attggaggtg ctgctattaa
     4861 agcatactct ctaccaaata acatactacc cactgttaaa cagccaaccc ctattcctac
     4921 aatacctact aatgcaatta caaccgtttt ccattcatga cataagtctt caagatttat
     4981 aagtgttcct aagttagtta ttaataaagc tataccaaag ttgctcattg cagctaatat
     5041 ccctgatgct cctgtaatgt cttttggtaa aacatttgtc caaaatccta gtaaaaatat
     5101 aatacataca aataacaatg ttgaaattgc accttttgtt ttcttagata aaaactcact
     5161 aaaagcataa atcattacac atacagtaaa gcaagtaagt ggattccaaa aattttccat
     5221 tgttgtttcc tcctaaatat aataattttt tatcaataaa acaataatta taaaatgtat
     5281 attatgcagt tattct
//
LOCUS       DACTGW010000230         5269 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.232, whole genome shotgun sequence.
ACCESSION   DACTGW010000230 DACTGW010000000
VERSION     DACTGW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.232"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            522..1829
                     /locus_tag="I9573_003170"
     CDS             522..1829
                     /locus_tag="I9573_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797765.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLVLDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQSKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     gene            2303..3106
                     /locus_tag="I9573_003171"
     CDS             2303..3106
                     /locus_tag="I9573_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT4797766.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINKTLKEMKKDGTFDKIYDKWFK"
     gene            3144..3755
                     /locus_tag="I9573_003172"
     CDS             3144..3755
                     /locus_tag="I9573_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAT4797767.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3787..4458
                     /locus_tag="I9573_003173"
     CDS             3787..4458
                     /locus_tag="I9573_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAT4797768.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4757..5119
                     /locus_tag="I9573_003174"
     CDS             4757..5119
                     /locus_tag="I9573_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAT4797769.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         2133 a          463 c          926 g         1747 t
ORIGIN      
        1 taagtaaaat atattaacat aaaggtaaaa ttttgtatac taaaagggaa tatttacagt
       61 ttaatggcag ttttaaaata ataaattaaa gctgtcattt ttatttaaaa atttttagaa
      121 aaagtacaat agtgttaatg tgtgaattag ataaggtgta tataataatt tagatgatat
      181 gatataaaat atgttgtaaa ttaaatgttt tctttgacaa tatatacaca aggatgataa
      241 tatctatatg aataaaagat agtgtgtaat ttgataatat gtaattataa cattatgata
      301 taattacaat aaagtatgta ataatctgta gaaatttcaa gctagttatc taagttttcg
      361 gataactaaa tctaaacgga atacataatg tattaaataa atgatgatac taaaatgata
      421 ctatttttat agaaatttat tgaggtatat aattgatgaa aataatatat taaattatat
      481 aatatattga gttttacata aaagcgaagg tgggaataaa aatgaaagaa attgctgatg
      541 ctataacatc agagtattta agctatggta tcaatgaaga tattagtatc tatgaaggta
      601 gattttgcat atatttagat aaaaaatata gatgtaatgg gaaaatttat tataaaatga
      661 cacctccaat atctataagt tttaaagcag acataggttg tgttgaagag atagacaatg
      721 aagatgacaa tttagttttg gattatgata atgctattct tgaagttcat ggatacaaaa
      781 ttatttctat aacaataaat actttaagtg aatttagtgt agaaggttat atcaatgatg
      841 actgcataaa atctaagaac tcctatgttg aatatgtaga ttttaatata ataaacttag
      901 ataaaatacc tggaaaacta attaaataca atgataaggt atacgcaggt agaattgaat
      961 ttgatataaa tgattatgtt gtaactatag acaagcgata tgattataga aaagaattaa
     1021 aatcagaatt aaagtctaaa tctggagcaa taataactca tataggtagg attagaagaa
     1081 aagatggtag aatttttaga acaaataata caataaatct tttagataga atatcaacag
     1141 ctttgagttt tatgtgtgga agatatgtag ggttttgttt ggccaaaggg tatagaagtg
     1201 gtaatgaagt ttatagaata tggaatgaaa atcaaatatc tccttttaga tatgtaccaa
     1261 catggtcaga tacactttca aattatcata atatggaaaa atatataagc cttatgtgta
     1321 aaaagctaga agatttttat tatggttctg ctataaagag tgttgttgat tggtacatag
     1381 aatctcttgg aagtgctacc atggaaaaca atataatatc tgtacaaatt gcacttgaaa
     1441 ctttgtcata tgtaattcta gtggaacaaa gtaagatact tacagatgaa gcatttgact
     1501 gtaatttggc atctaaaaat ataagactct tacttgatac atgtaagata ccatatggta
     1561 aacatgaatt aaatatattt gataatataa taaaaaataa atttgatgat ggagtggatt
     1621 tagttattta tttaagaaat agcatagttc atccaagtag aaagactcat agagctgtgt
     1681 tagaggttga agatatttgg aatataattt caataggaac gagatatatt gaacttgttt
     1741 tactctttat tttaggttat agaggagaat attcaaacag attagtagaa aggtgttatg
     1801 gtgaagtgga agttgtacct tggaactaga tttttatatt tattttaaaa tatatattga
     1861 catatattgc cttatgctat aaaatcaata tcataataat aattcagaat aacattaaat
     1921 gctaagaaaa gaaatagtaa aggtagttaa tcttaaacag agagtttccg attggtgaga
     1981 ggagcaatag attttacttt gaacacatct ttgagcagga ctctgaaaga gtagaaatac
     2041 tttattagga gatttccgga atccttgcac ccgttatagt gctagagtat aataatttta
     2101 ttgtacttaa agaggttagt gtcgtgaggc attaatgaac agaggtggaa ccacgagagt
     2161 taactcccgt cctctagcag aagctagagg cgggagtttt ttaatataat tttttaagcg
     2221 cgcagagaat tatattgcaa tagatacact ttttaaaaaa taatcaaaat attttaaaaa
     2281 ttaaaaggcg agggagaata ttatgaaaaa tatattaaaa aaagtaggaa tatttacaat
     2341 tatgttaggt ttattaggtg gagttgtagg ttgttcaaaa ccagataatg aaaaagataa
     2401 ggatgcttca aaggaatcta aaaaagaagt agttgtaggg tttgataata cgtttgtacc
     2461 aatgggattt ttagatgaaa agggagatac agtaggattt gacgtagact tagctaaaga
     2521 aacatttaaa agacttggaa tggaagttaa gtttcagcct atagattggt caatgaagga
     2581 aactgaatta aatgattcaa aaactgtaga tgtattatgg aatggatatt ctataacaga
     2641 cgaaagaaag aaaatagtat catatacaga accatattta caaaataaac agataatagt
     2701 tactctaagt gattcaaaaa taaattcaaa agctgattta aaagataaag aagttggaac
     2761 acaacaaggt tcaacagcac ttgatgcagt ggaaaaggat aaagatttta tgaatagctt
     2821 aaaaggtgga gcaccagttc tttatgatac ttatgataaa gcacttagag atttagagat
     2881 aggtagaact agtgcagttg ttggtgatga agttttaatt agatattata tgggacaaaa
     2941 gggtgaagac aaatacaagg tattaaaaga cgattttgga ttagaagatt atgttgtagc
     3001 aacttctaaa gaaaatccag aactttgtga gaaaataaat aaaacattaa aagaaatgaa
     3061 aaaagatgga acttttgaca aaatttatga taagtggttt aaatagaaga taagttagct
     3121 gttaagaagg gggcactaaa ggcatgttac aaggtttaga gatagttata gcaatgtttt
     3181 gtataacatt gatagtatca attccattgg ggattggagt tgcattttta agattatcaa
     3241 agaataaatt agtaagtggt attacacaat gttatatatt gataatgaga ggtacacctt
     3301 tacttttgca aatgatagtc atattttacg gattacctct tttaggaatt gtatttgaca
     3361 gatttacagc tggagtagta gctttcttct taaattatgc agcatatttt gcagaaatat
     3421 ttagaggtgg aatacagtca atcgatagag gacaatatga agcttcaaaa gtactaggat
     3481 ttgataaatt tactatgtat aagagagtaa tctttccaca agtttttaaa agaatactag
     3541 cacctatttc aaatgaagta atcacattag taaaagatac atctttagta tatatattag
     3601 gattaaatga tattttaaga atatctcaaa tagcaatgaa cagggaagca agtctgttac
     3661 cattatttga ggcaggagcg atatatctta tatttgtagc tatacttact aagggatttg
     3721 aattacttga aaaaaaatat tcttattata gataaagtta caatattata ggaggaggtt
     3781 ttttatatgt tgaaaatcaa aaacttaaat aaatcattta aaaaaaatag agtacttaaa
     3841 gatatttctt ttgagttaga agaaggtcag attggagttt tacttggtaa gtctggtgca
     3901 ggaaagacca caatattaag atgtataaat ggtcttgaag aatttgatag tggagagatt
     3961 ataatagata atgaggtaat aaaaaataag agagatatgg caaaaatacg aggaaaaata
     4021 ggtatggttt ttcaaaattt caacttattt cctcatatga cagtccttga aaatataatt
     4081 gaatccccag ttaatgtttt taaagttcct agaaaagagg ctgaagagag ggctagagaa
     4141 cttttaagat tagttgattt agaagataaa ttaaattctt atccatttga attgtcagga
     4201 ggacagcaac agagagttgc tatagctagg tcatgtgcat tgatgccaaa agtcctttgt
     4261 tttgatgaac ctacttctgc acttgatatt gatactattc aaagagtagt caatataatg
     4321 aatagactta aagacaaagg tatgacaata ttaataatta cacatgatgt tgtcttctca
     4381 aataatgttg cagataaaat tataagtata aaagatggta tagtagaaaa tgttcaaata
     4441 aaagaaaaaa ttgtttagag ttttataaat ataaactagg gtagaatttt ataatataaa
     4501 taaagggcaa gtaaaatcaa atatactatg cccttttgtt atctcaaaat ttaatcttat
     4561 attttatgtt taagttacat tataatttat aataaaaata aaaggactat ttaaatatat
     4621 aatgtgaata aatgtttaat aatttgaaat atgataattt tttatgttga aagcttataa
     4681 tgattatgat ataaattggt tatagtatac aatttatggg tataacaata aaaaattaga
     4741 aaggaatttt tataaaatga ttaaattata tggatataca aaatgctcta ctgttaaaaa
     4801 agctaaaaat tggcttaagg aaaataattt agaatttgaa gatatagata tggttcaaaa
     4861 ccctcctagt aaagaggagt taaaatcaat ttataagact agtggatatg atataaagaa
     4921 attttttaat acaagtggta tgaaatatag agaattgggt cttaaagata ttgtaaaaac
     4981 tgaatctgat gataaacttt tagaaatatt ggtaagtgat ggtatgctta ttaaaagacc
     5041 attacttttg gatggtaaaa atgtattatt gggctttaaa gaagatgtat ggaaaagtac
     5101 tttgctaaaa gaagattagg aagagatagg tgtgttgatt aaaattatta attaatacac
     5161 ctataaattt atgtattaaa tctaatcttt ataaatatta attagcaaat tcaaatcaaa
     5221 aaaattcata tcaacaaata tattacttct tattacaaat tatatcata
//
LOCUS       DACTGW010000231         5252 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.234, whole genome shotgun sequence.
ACCESSION   DACTGW010000231 DACTGW010000000
VERSION     DACTGW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.234"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            203..3742
                     /gene="nifJ"
                     /locus_tag="I9573_003175"
     CDS             203..3742
                     /gene="nifJ"
                     /locus_tag="I9573_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAT4797770.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(4134..5057)
                     /locus_tag="I9573_003176"
     CDS             complement(4134..5057)
                     /locus_tag="I9573_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HAT4797771.1"
                     /translation="MRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNKS
                     LVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNKP
                     DNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPNNNKPESNKPEDNN
                     NSDSTNKPDDNNNSGSTSENFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKKS
                     QDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGHR
                     KNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
BASE COUNT         1823 a          711 c          968 g         1750 t
ORIGIN      
        1 ttaaaaaaaa tctacttaaa tatgtaatta atttacaatt ttcaaaggaa ttttatctaa
       61 tctgtagtat tatattagaa gatagagtat ttgcgcgact atatgtgtgt ttatattttt
      121 gttgtgtaaa ttatatgtaa atataaaaaa atagacttgt tataaacaag tacaattgta
      181 gctatcaagg aggatacata ttatggctaa gtttatgaaa acacttgatg gaaatacagc
      241 tgcagctcat gttgcctatg cgtttacaga tgtagcagct atatatccaa tcacaccatc
      301 ttcaactatg gctgaagtag ttgatgagtg ggcatcacaa ggaagaaaaa atatatttgg
      361 acaaacagtt aatgttgttg aaatgcagtc agaagcaggt gcatcaggaa cattccacgg
      421 ttctttacaa ggaggagctt taacttctac ttttacagca tctcaaggtt tattattaat
      481 gatacctaac atgtataaag ttgcaggaga gttattacca ggagtattcc atgtaagtgc
      541 tcgtgctttg gcatctcaag cgttatcaat atttggggat caccaagacg ttatggctgc
      601 tagacaaact ggatgtgtat tattagcttc tggttcagtt caagaagtag ctgatatagc
      661 acctgttgca catttagctg caatagaagg tagattacca tttatacatt tctttgatgg
      721 attcagaact tcacatgaaa tccaaaaagt tgaattacta gaaaatgaag attatgcaag
      781 tttattaaac tttgaagcag ttcaagcatt tagagataat gctttatctc caaatcatcc
      841 agttgctcgt ggtactgctc aaaacccaga tatatatttc caaactagag aagcttctaa
      901 taaatactat caaaatatag taggtatagt tgaaaaatac atggaaaaaa tgagtaattt
      961 aacaggtaga aaacatagtt tatttgatta ctatggagca gaagatgcta aatacgtatt
     1021 aatagctatg ggttcagtaa cagaaacaat agaagaaact atagattact taaatgctaa
     1081 gggagaaaaa tatggtttag ttaaagttca tttatataga ccattctcaa tgaaacattt
     1141 cttagatgct atgccatcta ctgttgaaag aatatgtgta cttgatagaa ctaaagagcc
     1201 aggttcaact ggtgaaccat tatacttaga tgtacgtgat gtattctatg gaaaagaaaa
     1261 tgctcctatg ataataggtg gaagatacgg attaggttca aaagatacta ctccttcaga
     1321 tataaaaact gtttttgata acttagtttc agaacaacct aaaaatggat ttacagtagg
     1381 tatagttgat gatgtaacta atacttcttt aactccatct gagccaataa aaatagcttc
     1441 taaaggaact ataagatgta agttctgggg attaggttct gacggtactg ttggagctaa
     1501 taaacaagct atcaaaataa tcggtgacca tactgaaaaa tatgctcaag cttactttga
     1561 ttatgactct aaaaaatctg gtggtataac tatgtctcac ttaagatttg gtgatactcc
     1621 aataagagct acttatttaa tagatgaagc tgattatatt gcctgtcata aacaatcata
     1681 tgtttatcaa tatgatttat taaaaggtct taaaaaaggt ggtacatttg tacttaacac
     1741 tatatgggac caagcaggac ttgaagaaca tttaccagct catatgaaac aatatatagc
     1801 taaaaatgat ataaaattct atacagttaa tgctgttaaa ctaggacaag aaataggtct
     1861 tggaaacaga ataaacatga taatgcaatc tgcattcttt aaattagctg aaataatacc
     1921 agaagaagat gctgttaaat acttaaaaga ttctatagtt aaagcttatg gtaaaaaagg
     1981 tgaaaaaata gttaatatga actatgctgc tgttgatgca ggaataaatg ctttagttaa
     2041 agttgaagtt ccagcttctt gggaaaatgc tgttgatgca gataaaggag aagttaaaga
     2101 accagaattt ataaaaaata tactaagacc aatgactgca caagaaggaa acaacttacc
     2161 agtaagtaca ttcaatggta tagaagatgg aacattccct tgtggtactg cagcttatga
     2221 aaaacgtggt atagctgttg atgttcctga atggataata gataactgta ttcaatgtaa
     2281 ccaatgttct tttatatgtc ctcatgcatg tataagacca gtattagtta ctgaagaaga
     2341 attagctaat gctccagaag gatttgaagc taaaaaagca ttaggtaaag gattagaagg
     2401 attaaaatat agaattcaag ttagtccact tgactgtaca ggatgtggaa actgtgctga
     2461 catatgtcca gctaaagaaa aagctttagt tatgaagcca atagatactc aacttgatac
     2521 agagttagat aattgggcat ttgcagtaaa tcctgatgaa gtagctccaa aagttgatgt
     2581 tatgcctgca aatactgtta aaggaagtca atttagacaa ccattaatgg agttttcagg
     2641 agcatgtgct ggatgtggag aaactgcgta cattaaagta gttacacaat tatatggaga
     2701 tagaatgatg atagctaatg ctactggatg ttcttctata tggggagctt ctgcaccatc
     2761 aattccttat acttgtaacc atgaaggtaa aggtccatct tgggcaaatt ctttattcga
     2821 ggataatgct gaatatggat ttggtatgta cacagctgtt aagcaaataa gaaataaaat
     2881 agtagatgct atgacagagt tagtttctat ggatatctgt gaagatgcta aagcagtatt
     2941 tacagagtgg ttagattcta gaaatgatgg agaagcttct aaagtagcaa gtgctaaggt
     3001 tgttgaatta ttagaaaaac cagcttgtga ttgtacagat gaaaaagcta aagaattagt
     3061 aaaagctata aaagatagaa aagattatct agttaagaga tctcaatgga tactaggtgg
     3121 agacggttgg gcatatgata ttggttacgg tggattagac catgttcttg catctggtga
     3181 agatgtaaac gtacttgtat ttgatacaga agtatattca aatacaggag gtcaagcttc
     3241 taaagctact ccagctgctg ctatggctaa gtttgctgct tcaggtaaaa aatctagaaa
     3301 gaaagattta ggaatgatgg ctatgacata tggaaacgta tatgtagctc aagttgctat
     3361 gggggctgat aaaaatcaat ttatcaaagc cgttttagaa gctgaaaaac atgatgggcc
     3421 atctctaata atagcttatg ctccatgtat aaaccatgga ttaaaagaag gtatgggaag
     3481 aactgtggct aatgaagcac aagctgttgc ttgcggatac tggcatttat acagattcaa
     3541 tccagaagta aaagctgaag gtaaaaaccc attcacttta gattcaaaag aaccaactgc
     3601 aagcttcaaa gatttcatac ttgcacaagt aagatattct gctatagcta aacaattccc
     3661 agaggaagct gatggattat ttgctaaagc tgaagctgat gctaaagaaa gatatgaagg
     3721 atacaaaaaa ttagctgaat aatattaaag tattataatg cttatataga attgataaaa
     3781 gagagtgtct caaagtgagc tttttagttc atgaggcact ctttttgtat gaaatcaaat
     3841 atattttaat catttcaaca atgaaaaagg ccttatctct attttgagac agcctttttt
     3901 tatgaagata ttaagatcct gtttttacct attaaatatc tttttaagtt ttccattttc
     3961 atcttgccaa tactttttat atattgttct acacactaaa attaagagaa caaataatat
     4021 ttatagtatg tatatttgga tttgataatt ttaaaataga tacaaaagag tatctcattt
     4081 gtgtttttta tcacttagag atactctttt gatatgcatg gaaaaagagt attttatcct
     4141 acaaagattt gtgtccaata aattgaacca ccactttttt gtgctactcc aacacctatt
     4201 ttactgaaat ttggattcat tatatttttt ctgtgacctt cagaattcat ccatgcgttt
     4261 acaacttctt taggagtttt ttgacccata gctatatttt cacctgctgt cttatagctt
     4321 attccaaatt ttttcaacat gtcaaatgga gaaccataag ttggagaatt gtgtgaaaaa
     4381 tagttgttat caatcatatc ttgagatttt tttgtagcaa catttgatat agaagaatca
     4441 agagtaagag gatttaatcc agctttagct ctttctatat ttactaagtc gacaacttct
     4501 ttttggtaag ctgaaaaatt ttcacttgta gagcctgagt tgttattatc atcaggtttg
     4561 ttagtagagt ctgaattatt gttgtcttca ggtttattag actctggttt attgttatta
     4621 ggtttatcag tagagcctga gttattgttg tcttcaggtt tattagactc tggtttattg
     4681 ttatcaggtt tattagtaga gcctgaatta ttgttgtcct caggtttatt agactctggt
     4741 ttattgttat caggcttatt agtagagtct gaattattgt tgtcctcagg tttattagtg
     4801 ttgttgcaat tcttgtcatt atgattctta tccttacaag agttatcttt atcaggttta
     4861 agttctggtt ttacagaaca atttggtttt gcagaaaact tgtatttagt attactatat
     4921 actaaacttt tgttacttat atttacatta ttttcaagtg atgaagcact tgcagtaaca
     4981 gcacttgaag ctgttgctaa agttaacact cctaaagcta atatagtttt taattttgtt
     5041 ggtatatttc ttctcatcat taaacacctc ttaatctaaa aattctaaaa atgttactga
     5101 tttgtaacct ttagtgtagg gggtacccgt gaatctatac taacataaga ttaagacttt
     5161 ttttactgta aagatatgta aaaaagatat atatggaata aataggtata tttcagtgtg
     5221 tttgaaaatt tgtattaaaa gcctgtatag ta
//
LOCUS       DACTGW010000232         5243 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.235, whole genome shotgun sequence.
ACCESSION   DACTGW010000232 DACTGW010000000
VERSION     DACTGW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.235"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            107..1012
                     /locus_tag="I9573_003177"
     CDS             107..1012
                     /locus_tag="I9573_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HAT4797772.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            1021..2205
                     /locus_tag="I9573_003178"
     CDS             1021..2205
                     /locus_tag="I9573_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4797773.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     RIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNTYINE
                     YI"
     gene            2320..3621
                     /locus_tag="I9573_003179"
     CDS             2320..3621
                     /locus_tag="I9573_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HAT4797774.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIESPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            3575..4618
                     /gene="holA"
                     /locus_tag="I9573_003180"
     CDS             3575..4618
                     /gene="holA"
                     /locus_tag="I9573_003180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAT4797775.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(4805..5071)
                     /gene="rpsT"
                     /locus_tag="I9573_003181"
     CDS             complement(4805..5071)
                     /gene="rpsT"
                     /locus_tag="I9573_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAT4797776.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT         2112 a          468 c          822 g         1841 t
ORIGIN      
        1 attttaaaca atataaatga aattaatacc ataataaatt tggagttttt tatatataca
       61 aaataaaatt tttaaaatta agcaataaaa aaggtaggag gattttatgt ttttcaaaga
      121 tattgaacta aattcaaaaa aagagttaga cccatatttt gatttagtag attatgaagc
      181 ttgtgagtac tgttttagta ctttatatat gtggcaacat gtttataaga caggatatta
      241 tataggtgaa gattttgccg ttttagttgg ggaatacgaa ggagatagtt tctcaatatt
      301 accacttgct aaaaaagata aacttccaga agttgtagat tttgtccttg aatatttttc
      361 taaaaataat aaaaaaatat acttaagagg tattactact gaagtagtag agtttttaaa
      421 agaaaaatat ccaggtagat ttgagtatat agaagaaaga gatttatttg actatattta
      481 tgatgcagaa agtttaagaa ctctagctgg taaaaagaat caaaagaaga gaaaccatat
      541 aaactatttc ttaaaagagt atgcaggtag atatgaagct aaattattag ataaagaaaa
      601 ctttgacgag tgtttagtac taatgaagga atgggaaagt aataaggaag aaaataatga
      661 atttgatgaa agtatggatg acgaattaat aggtatcaaa aagatattta accattatga
      721 catattaaaa gataaggtaa aagtgtttgg tgtttatgtg gatggaaaat tagaggcttt
      781 tagtatagga gaacttttaa atccaaatat ggcattgatt catatagaaa aagctaatcc
      841 cgatataaga ggtctgtatc catttataaa tcaacaattt ttggtaagtg aatttaagga
      901 cgttgagttt gtaaatagag aagaagattt aggtattgaa ggattaagaa aagctaaact
      961 ttcttatcat ccttgcagat ttgttgaaaa gtacagtgtt agggaggctt aatagactat
     1021 atggagatta ggtatgctaa agaagaagag atagaaagta taaaagagat ttggagttat
     1081 tgttttaacg atacggagag ttttatgaag tactatttta atgataaata taaatctgaa
     1141 aatactgtag tagctttaga tgaaggaaaa attatttctt ctcttcagct aaatcagtat
     1201 aagttattac taaatagcaa ggtatataat acatcatatg tagtaggagt ttctacactt
     1261 ccagagggaa gaggagctgg atatatgaac aaagtaatga aatttacttt aaacgaattg
     1321 tataaaaaag ggcaattagt ttctatattg atgcctattg actatagact ttatagaaga
     1381 tttggatatg agcattgtta cgaccagatt gaatatacta ttaacacaga tgatttaaaa
     1441 aattttaagt caagtggaaa aatgataaag tctaatttat cacaaattga tgatttgata
     1501 cgaatagata gaactttttt aaatgaggtt aatggtaatg tgttaaaaga tgaacattat
     1561 tatgaaaact tatttaaaga gattcaaagt gaagatggat ttttatacat tcatgaagga
     1621 aatgaaaaag atggatatat agtttatttt cttcaggaag ataaaatgtt tgtcagagag
     1681 ttattttata aaaatataga tgcccttaaa tcaatgctta aatttatata taatcacaat
     1741 actcaatgta agatagttac tatatctact cctacaatag ataagataag atttatatta
     1801 gacaacccta aagatagtga tataaaaata aaacctttta tgatgggaag agttataaat
     1861 gtaaagaagt ttattgaaga tatagatatt gagaaggata ttaatagctc atttaattta
     1921 ttaatagaag acaagtttat agatgaaaat aatggtctat ttaaaatttc tatacagaat
     1981 aagaaggtat ctgtagagca gttagataaa aaaggtgcag aaaagcctca agaagatttt
     2041 gatattaaat tagatataaa taccttaaca cagttatcat tttcttatat agatgtaaat
     2101 gaagctattt tcttaaatga tataaaagat gtttcagaag aaacactaga aacacttaat
     2161 tgtatttttt ctaaaaaaaa tacctatata aatgaatata tataggtaga atacgttaaa
     2221 agattttaaa aatcttttga cgtatttttt tgtttagata acaatttatc ttgtttaaaa
     2281 attttctatt tgaaaataat ctaaataata tacttttata tgggagtgat tataattcaa
     2341 ataagaagac cattattaat tatattaatg tttgtggtag ctatatcatt tatcattaca
     2401 aataacacaa aaaacgacga agaacttaat gataagataa taactataca aggggttgtc
     2461 aaaggaaaaa ttgaaaagaa aagatacaat cagtacaagg taggggtttt tctaataaat
     2521 gattatacaa aaaataaaaa tttgaagatt ggtcagaaag ttaatattac aggtaaattc
     2581 aaaagtttag ataaaatgaa gtatgatgat tttgattatg gaaggtatat aaaaagtact
     2641 ggatataaag gtatagtgta tataaataac tataaaatta ttgataaaaa taaactttac
     2701 tctttaatag gggaaataaa gttttatatt agtaaaactt ataggtactt atataagaaa
     2761 aactcagatt ttataaattc tatactatta gcagaagttg aaaatttgac tgaggaacaa
     2821 aaagaaattt ttactagaac tggaactagt catgtaatat ctatatcagg tcttcataca
     2881 ggaatattat gtgtaattat atcctttcta ttaagaggta taaataaatt gtataaatta
     2941 ttaatactgt cgatttttat aactttatat tgtataatgg taggtgcgtc tccatccata
     3001 attaggtcta tagcatttgt tatgattttt tatttatcaa tatttattga tagaaagaaa
     3061 gatggaatat cagcattgtc tctaattggg ataattttaa ttatgaataa cccctatgtt
     3121 atatataatg taagttttca attgtctttt ttagctacac tttctatatt aatattctat
     3181 aataaaataa atagtattat aaaattaagt atggtatcat tgacaatatc atcaaatata
     3241 ttaactttac ctataattta ttatacattt aaaggtatac cattgctttc aattatagga
     3301 aatttaatta tagtaccatt tgtgggagta ataatgtatt taagtattgc aagtttaatc
     3361 gtatttaaag taagtgtagt aatagctaaa attatatctt tttttaatag tacattgata
     3421 gaaagcatat tttttttact agaaaaaatc agcaatttaa gttttgcata cattaatata
     3481 gaaagtccta aattttatat tgttgttatt tattacatag gagtgttttt ttacatattt
     3541 tacatagaag gaaaagagat aaaggagcaa gagaatgaat tacaaggata ttataaggaa
     3601 tgtaaaagag aaaaactttg aaaaaatgta tcttttttat ggaagagagt attatttaat
     3661 tgagaatgca ataaaagcat ttaaagatag tctaaacgaa ggaatgcttg attttaattt
     3721 agatataata gatgggaagg aaatagtctt aaatcagctt ataagctcta tagaaacact
     3781 tccatttatg gatgatagaa agatagtaat aataaaagac ttcgaactat taaaaggaaa
     3841 gaaaaagaat tttacagata gtgatgagaa atacttaata gagcatttag ataatattcc
     3901 agacactaca actatagttt ttgttgtata tggagatgta gacaaaagaa aatcattagt
     3961 taagaaaata ggaaacaatg gaattgtttt tgattgtgat aagctttctg atatggattt
     4021 atttaaatgg ataaaaaaat cttttgcttt aaatgatgtt ataatagata attctcaaat
     4081 catgtatttt attgaacaag aaggatacag agataagagc agtgaaaaga cattatctga
     4141 tttagaaaat gaaataaata aaataagttc ctttgttggt aaagggaata atgtaaccaa
     4201 tgacgtaatt gataaattat ctcaaaaaaa agttgagaat gatatattta aattaatcga
     4261 ttatattgga gaacaaaatg cttctaatgc aatgaaaata ttaaatgata tgatacaaga
     4321 aggcgaatct gtattaggta tattttctat gatagcaaga caatttaaaa taattatgca
     4381 agtaagacag ttacaacttg atggatattc aacaaaactt atagctgata aattaaaaat
     4441 gcatcaattt gttgtaggaa aagcattaaa gcaaactaaa aatttttctg atgatataat
     4501 tgtggaaata ttaaactata tattagaaag tgattataaa ataaaaacag gtcttataag
     4561 agatacttta gcagttgaaa tgttggttag tagatactgt aaaagagaag caatttaaaa
     4621 aatctatata ttaataaaaa ataaatagca agatatgtta tagttcagag tagatgtaag
     4681 ctaaattaag tttgatatac aatcaactat aatatgtcta actatttatt ttttattgta
     4741 taaatttaca aaataaaaaa cccctacata ggggttatta tatataagaa atataaactg
     4801 gtaattaagc gttcataccg tttaattttt gagctaattt agctactttt ctagctgcag
     4861 cattcttatg tatagttcct ttagaagcta cttggtatat tcttttttga gcgtattgga
     4921 actttgcaac agcatcctct ctgtttccag ctgcaacagc atcttcgaat cttcttattg
     4981 cagtttttat ttgagatttt ctaactctat ttaaagcagt tttcttttcg ataacactta
     5041 ttcttttctt agctgatttt atatttgcca ccgtgttcac ctccctgtat ttttttatca
     5101 tattcatcgt caacatatta gattttaaca gaaataaatc taaaaatcaa ggattattat
     5161 ttatatattt taataatgtt ttttattttg attttttaga ctgtaaattg tgtataaata
     5221 atttgcaata tcttgttttt aaa
//
LOCUS       DACTGW010000233         5143 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.236, whole genome shotgun sequence.
ACCESSION   DACTGW010000233 DACTGW010000000
VERSION     DACTGW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.236"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            181..1359
                     /locus_tag="I9573_003182"
     CDS             181..1359
                     /locus_tag="I9573_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAT4797777.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINSGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            1368..2024
                     /locus_tag="I9573_003183"
     CDS             1368..2024
                     /locus_tag="I9573_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HAT4797778.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            2024..2689
                     /locus_tag="I9573_003184"
     CDS             2024..2689
                     /locus_tag="I9573_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HAT4797779.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     ILGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            2788..3570
                     /locus_tag="I9573_003185"
     CDS             2788..3570
                     /locus_tag="I9573_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HAT4797780.1"
                     /translation="MKMVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALN
                     NTITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDF
                     PTDKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKH
                     GLIGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYP
                     LVPQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            3632..4966
                     /locus_tag="I9573_003186"
     CDS             3632..4966
                     /locus_tag="I9573_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HAT4797781.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVLLNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKVGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSKLEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
BASE COUNT         1838 a          732 c          934 g         1639 t
ORIGIN      
        1 caaaatgctt ttttcgccgt aatctataat tagaaacagg aaaacctaaa gcattaggaa
       61 taaaagtaat tgaaatactt aaaaacaagc ttgtattaaa aaagtacttt attatagtgt
      121 tccctaattt ctttattcag aaaattttta attatcattc aagaaaggta actattagat
      181 atgaaagatg tagtaatcgt atcagcagtc agaacaccta taggaagttt tggaggtgta
      241 tttaaaaata cttcagctgt tcaacttgga actatcgctg ttaaagaagc gatatcacgt
      301 gtaggactta atttatctga aatagatgaa gtaataattg gaaatgttct tcaaacaggg
      361 cttggacaga atgttgctag gcaaattgct ataaattcag gtataccaaa ttctgttcca
      421 agctacacag taaataaatt atgtggttca gggcttaaaa gtgttcagct agctgcccaa
      481 tcaatcacat ctggtgaaaa tgatgtggta attgcaggtg gtactgaaaa tatgagccaa
      541 gctccttata tcgttccaac tgctcgcttt ggtagtaaaa tgggtgacat aaccatggta
      601 gatagtatgc taacagatgg ccttattgat gctttcaatc agtatcatat gggaataact
      661 gcagaaaata ttgcaactaa atttgaattt actagagaaa tgcaagataa attggcatta
      721 gaaagtcaaa ataaggctga gaatgctata aaaaacaacc gctttaaaga agaaatcgtt
      781 ccagtagatg ttttaatacg tcgaggaaaa atagaaacta ttgataaaga tgaatatcct
      841 aaattaggca tgacatttga aggtttagct aaacttaaac ctgcattcaa aaaagatggt
      901 actgtaacag ctggaaatgc ctcaggtatt aatgatggtg cggctatgtt aatactaatg
      961 agccaacaaa aagcagacga actaggtatt agaccacttg ctaaaataaa atcctatgct
     1021 tcagctggtg ttgaacctga agtcatgggt actggtccaa tccctgcaac tagaaaagca
     1081 ctgaaaaaag ctggacttag catcaatgat atagacttaa tagaagcaaa cgaagcgttt
     1141 gctgcccaag cattagctgt taaaaatgaa cttcaaatag attcatcaaa gttaaatgtt
     1201 aatggtggtg caatagcatt aggacatcca ataggagcaa gtggagctag aatacttgtt
     1261 accttaattt atgaaatgca aaaacgtaaa gttgaaactg gacttgcaac actttgtatt
     1321 ggtggtggtc aaggaatatc aatggtggtt tcacgatagg aggaaatatg aataaaatag
     1381 ttagcattga tgaagctcta tctcatgtaa aagatggaga tacaataatg gttggtggat
     1441 tcatggccaa tggttcacca gaaaatttaa ttgattattt atgtagtaaa aatataaaag
     1501 atttgacact aatttgcaat gatactggct ttattgacaa aggagttggc aaaatggttg
     1561 taaataagca atttaaaaag ataattgctt ctcatgtagg attaaataaa gaaactggaa
     1621 gacaaatgaa caacaatgaa acagatgttg agttagttcc tcagggaact cttgctgaac
     1681 aaatacgtgc agctggttat ggtctaggag gtatactcac accgactggt attggtactc
     1741 ttgttgagga gggaaagcaa aaaattgttg tcgatagcaa agaatacctt cttgaaaaac
     1801 caatttatgc agatgttgct cttctatttg catcaaaggt cgataaagca ggtaatctag
     1861 tttataaagg ctctatgaac aattttaata atttaatggc atctgctgcc aaaattacaa
     1921 tagttgaagc agatgaaatc gttgaaattg gtgacattga cccaaatgaa gttaatacac
     1981 caggtatttt tgtgaattac atcgttgaag gaggtaattt ataatggata aattagaaat
     2041 gcaagaatat attgcaaata gagtatctaa agaattaaaa gacggagccg ttgtaaacct
     2101 tggaataggt ctaccaacca aagtagcaaa ttatattccg gataatgtaa acgttatctt
     2161 acaatctgaa aatggctttt taggtttagg ttcagccgaa gatggaaatt caagtgatga
     2221 aactattgta aatgcaggtg gtcaaccagt tactatactt cctggaggat gtttctttga
     2281 tagtgctact tcttttggaa taatcagagg aggtcatgtt gatataacta ttcttggcgc
     2341 acttcaggtt gacaaatatg gcaatatagc aaactatatg attccaggta agatggttcc
     2401 aggtatgggt ggagctatgg acctagtaac tggagctaaa aaagttattg tagcaatgga
     2461 acatacttca aaaggttctg caaaaatatt aaataattgt actcttccac ttactgcaac
     2521 aaatgctgta gatttgatag ttactgaaat gggagttatg gaagtaacat cagatggaat
     2581 tttacttaaa gaaataaatc cagctttcac attagatgat gttatttctg ctactgaagc
     2641 tcctttaata ttgtcagatt cattaaatgg aaaaatatct gttgtttaaa ataattaaaa
     2701 ctagaaaata aaaatctctt agacgtatta catataaatt tgtattgctt aaaatattaa
     2761 tataaaaata aaataaaatt ggaggaaatg aaaatggtta aagataaagt tgtattcgta
     2821 acaggagctg caagtggaat aggtaaacaa atagctgaat cgttcttaaa aaatggtgct
     2881 aaagtaatgt tctctgatat aaatcaagaa gctttaaata ataccataac agagttacaa
     2941 aaaaaaggtt atgattgcat gagtgtaaag tgtgatgtaa ctaaggaaga agaaattaac
     3001 cacgctatag ataaaactgt tgaaaagtat ggcagattag atatattaat aaacaatgct
     3061 ggacttcaac atgtttctat gatagaagat ttcccaactg ataaatttga atttatgata
     3121 aaaataatgc tgacagcacc ttttatagct actaaaaaag catttcctat aatgaaaaaa
     3181 caaggctttg gtcgtataat aaatatggcc tcaatcaatg gtgtaatagg atttgctgga
     3241 aaggctgctt ataactcagc aaaacatgga cttattggac taacaaaagt tgctgctctt
     3301 gaagctgccg attctggtat aacagttaat gctttatgcc ctggatatgt agatactcca
     3361 cttgtcagag gtcagtttaa tgacctttca aaaactcgta atataccatt agaaaatgtc
     3421 ttagaggaag ttttgtaccc acttgtacca caaaaacgcc tattagatgt tcaagaaatt
     3481 gccgactatg ctatgttcct agcaagtgat aatgctaagg gtattacagg acaagcttgc
     3541 ttactagatg gaggatatac tgcacaatag ttattatatt tagggcttga agaaagtatt
     3601 cttcaggtcc ttctactaag gagggatatt tatgagtatt atcgcaccaa ttggtataat
     3661 tattggtatc atagctatta tatatttttc agttaaagaa attcatataa ctatagctgc
     3721 tccaatagca actttaatag ttgttctctt aaataaaatg gatgttgtaa catcaatgct
     3781 aggtgctgac aaaaacaatt atatgggtgc acttggaaac tatataatga gttattttgc
     3841 aatattctta ttaggctcaa tacttgctaa atttatggaa gaaagtggag ccactgtatc
     3901 aatagctgat tttattttaa ataaagttgg ctctaatcat ccttatagag ttttagttgc
     3961 tatatttata gtaagtttta tattaacgta tggtggtata agcttatttg tagttatgtt
     4021 tgcagtcatt ccacttgcaa gaactctatt taaaaaactt gatatttctt ggaaccttat
     4081 tcagatacca ctttggcttg gtattgcaac aattactatg actataattc ctggaacacc
     4141 agccattcaa aatgttatac ctattcaata tctaaatacg tctcttactg cagctactat
     4201 accaagtatt gtaggtagta taggttgtgc tacgtttgga cttgtctata tgaaatatgc
     4261 tctaaacaaa agtattagaa aaggtgaaac ttacgcaact tacacttcaa aattagaaga
     4321 agaatcttta gatagagaat taccaggatt ttttgcaagt atttttcctc tattagcact
     4381 cgtaattata gctataactg gaagtacttt tggttcagaa tttatgaaaa aaaatgtaat
     4441 ttacatagct ttaatagttg caattctttt atctgctttt ttattccata aatttctatc
     4501 atctaagata cagacattga gcataggtgc tagtggttct ataggtccta tatttgctac
     4561 atcttctgca gttgcatttg gagcagtaat aatggctgca cctggttttg atgtatttag
     4621 caaacttata atgtcaatac caggtagtcc acttataagt cttactgttt tgacttcaac
     4681 aatgtcagca ataacagggt cctcttctgg tgcattagga atagtaatgc caaactttgc
     4741 agattattat ttaagtactg gtctaaatcc agaattaatt caccgtgttg ctacaatagc
     4801 ctcaaatata ctaactatag ttccacaaag tggtgttttt cttacattct taagcttatc
     4861 taagttaaca cataaaactg gatttaaaga aactttcatt gtagttgcag ttggttcact
     4921 aatagcagaa attattgtaa ttacattagg attactaatg tattaatata aaacaaatta
     4981 aaattcataa tatgaaaggt ggtgtctcaa agtgaacttt ttacttaatg agacactctt
     5041 tttgtatgaa atcaaatata ttttaatcat ttcaacaata aaaaagaacc tttctctatt
     5101 ttgagacaaa ccctttttat tccttaaaga tttaaacaat tat
//
LOCUS       DACTGW010000234         5133 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.237, whole genome shotgun sequence.
ACCESSION   DACTGW010000234 DACTGW010000000
VERSION     DACTGW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.237"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            122..1648
                     /locus_tag="I9573_003187"
     CDS             122..1648
                     /locus_tag="I9573_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT4797782.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NNGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1676..2233
                     /locus_tag="I9573_003188"
     CDS             1676..2233
                     /locus_tag="I9573_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797783.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2755..3798
                     /locus_tag="I9573_003189"
     CDS             2755..3798
                     /locus_tag="I9573_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797784.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKG
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            3909..5051
                     /locus_tag="I9573_003190"
     CDS             3909..5051
                     /locus_tag="I9573_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAT4797785.1"
                     /translation="MSEEKNPKENTLDEPKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2165 a          423 c          919 g         1626 t
ORIGIN      
        1 caagaaaact aagtgatttt tcatttagct ttttattaag cttatttttt atactattta
       61 taataaataa actccatgat gggaaaacta ttactattta atatgtaaag gagtatttta
      121 aatgaatgaa tcatattggt ttttaaactc aagtccaaaa gaatataaca aattaggtga
      181 aaatataaaa acagattgtc ttatagtagg tggtggaata actggattga caactgcata
      241 tttattagca aaagaaggta aaaaagtagt gcttgttgag gctgataaaa taggatatgg
      301 aacttctgga aggaatactg gtaaggtaac ttgtcagcat gatatatttt actctaagat
      361 agagaaaaaa tatggattag ataaagcaaa atcatattat aatgcaaata atgaagcttt
      421 aaatttagtt gaacaaataa tagaagaaaa caatataaaa tgtgatttta aaagggaaac
      481 ttcttttgta tttacagaaa aagaggatac tataaagaat attaaagatg aatatcgtac
      541 atgtaaaaaa ataggaataa attgtgagta tcatgaaact atagaaaata ttccattaga
      601 tataaagggg gctattagtt ttactaatca aggtcaattt aacccaaaga agtatattga
      661 tggattggca aaagcagcag taaatttagg gctaaaaata tatgaaaata caccagtagt
      721 agatcttgaa aaaggaaaaa tatgtagggt taaaacaaga gaagataata taatagaagc
      781 tgagaatgtt ataatttcat ctcatagtcc atggtatgat ggattgaatt tttattttgc
      841 aaaagaatat gcagagcgtg cttatttaat ggttgcagtt ttggaaaata aattagcaga
      901 tggtatgttt ataagtatag atgacccatc aataactttt agacaatata ataatggaag
      961 tgaaaattta ctgatttttg gaggaggaga ccataaagtt ggccagggag gaactgagaa
     1021 agaaatattt gatgatttag agcattatgg taaagaagtc tttaaggtaa aagattttaa
     1081 ggggaaatgg tctgctcaag ataatatgag ttttgacaat gttccttata ttggttatat
     1141 caacaaaaga gaagacaata tttatgtagc tacaggattc tctaaatggg gaattactaa
     1201 tggaactgct gctggaataa taataaaaga tttgataata aataataatt ctgattataa
     1261 agatactttt aatccatcca gattaggtag ctacttttca aaagatttta taaaggaaaa
     1321 tgcaaacgta gcaataaatt atgtaagtgg aaaattaaaa ataggtagtg gagatatgcc
     1381 taaaaataat ggagaaggaa agattgttaa tattgatgga aaaagatatg gtgtatataa
     1441 ggatgataat ggagattttt atattgtaga cacgacatgt actcatttag gatgtgaact
     1501 taattttaac agtgaagaaa aaacatggga ctgtccatgt catggttcta gatttgacta
     1561 taagggaaat atacttgaag gaccagctct taagccatta aaactttatg gacatggtga
     1621 taatgatgtt aatcctaagt tattataaaa ttatttttag gaaggtaaag tgtatatgaa
     1681 taaactttta aagccaataa ctatactatt gtatatagtt tatattttat ctgccgtatt
     1741 ttttgtagca aacaaagaat atggaagtct aggtctagta gcaattagtt tagttggtac
     1801 tttcattttg agttttataa ataagcaaac tgaaagatta ttgagtaaag aattatatat
     1861 aatgcttgct ttatttataa tgttttcatc attgttaggt acttgtttta atttttatga
     1921 tataaattat tatgatgatt ttttgcattt atggtcaggt ataatatctt gtattgtggc
     1981 tttttctata attaaatact tttatacaca aagagagata agtaaaatga gtaaagtgtt
     2041 tttagttata tttttattta tgtttagtat gggagtagcg agtttatggg aaataggtga
     2101 atttttaatg gatacttttg ttgggactac tacacaggcc ggggggttag aagatactat
     2161 gattgatatg gtagatgctt taataggcac aattataact attcctttta tagtaaaaaa
     2221 atctaatata tagttttaaa ttttttatat taattataat ggatttaatt aatcatgttg
     2281 agtttcaagg tctatatgat taattaaatc catttttgtt atttataatt attattgaat
     2341 gtatttttac atataagata taaggtgata gatagaaatt gttggattat gtaaataatt
     2401 aatatataat accattatgg gcaataaata caaatcaaat gataatatag tctatttatg
     2461 taaatgttat attgtatcct aagtataata gtaagttttt tgatagtggt gtagaaagta
     2521 aaaatctact agcctaaatt gatgtatgga ttagtttagg aacagtaatt gcatatctta
     2581 agtttgatgt catgttgggc tactagaaat aggtcatctt tttcacttga atatcttttc
     2641 tttaaacatg aggagtatca tatcaaaaac atatgaaaat taagtgattt ataagaacag
     2701 taatatttta tatgatagta tagaatagta ataaagtatt aggaggaaca ttgagtgtta
     2761 aaaaagtggt ttggtattgt gaaaaaaaga caaaaaagtg agtcagtaaa agaagaaggt
     2821 gaagtaatat taaaaaacga aaaaatatta tctgaagaaa agttgataga tgaagaagga
     2881 gttgtagtta atattgataa tgaaatatta actaaagtag aagtagtaaa tgatgataat
     2941 gaaatagaag aaaaaataat agaagaagat tggttaatta gtgaaaatac cataaaatta
     3001 gatgataaag aagcaattat taatgataaa aacatagaat tatgtaaaaa agaagttcaa
     3061 gttgaaagtg aaaaaatagg tttaaataag tttgaaggac ttgaccaaaa tgaaaattat
     3121 aatctagaaa aaaatgttat tgaagaaaaa aaagtaagtg aatgtttgac agaagaagat
     3181 ttagagtaca taaaagaaat taaaataaaa agaggaaaaa gtataaaagc tataaacttg
     3241 tatactaaag aagagtgggt ttttgacact catatacagt gtagtaaaaa actcaaagtt
     3301 ccattaggat acataagaga aaatttaaaa tatggatata tggattactt tggagatgca
     3361 ataaattatt taagtgaagt attaaatata gatgaatact gcaaaggtga atggagctat
     3421 ctcgataata gcaaatctcc atctgagata tttaatattc taaacaataa aatatttagt
     3481 ataaggcttt ctaatgaaaa aagaaatgaa attttgacaa atgataaaat tgaagcatta
     3541 aaaatgaatt atagatttga atgtattgat gaagaatatg atgaatattt taaaaaatat
     3601 aagtctataa tcaaaagggg tggaaagaaa aaagttgaat tagtaaataa aaaaggtgac
     3661 attttagaaa tatttaagtc tttagaagaa tgtgccattt atttgcaaaa ggaaaagaat
     3721 gaagttatac aaatgttaaa atatggagat acaaaagtag gaagaaactt tataaggtat
     3781 agtttgagaa gtatttaatt tcattttaaa ttcataagag tttcatttct gattcacaca
     3841 tttaatatta ttttaatgta aaataataaa ggtgaagtat tgcacaatta aaattaagga
     3901 gggtattaat gtctgaagaa aaaaatccaa aagaaaatac attagatgaa cctaaaaatt
     3961 cagataatgt aaattctaaa aaagatgatg tgtcagatga aaaaaatact gataacaaag
     4021 ttaaaaaaag ggaaataaag ctaaacaaca aaacacttaa agctattgta gcaattgttg
     4081 cagtaggagc tatatgttta ggtggaggct atgtttttgg taaagaagct ggaagaaaat
     4141 tgcctgctac ttcaaaacac tataatagca gtgaggtagt agctactatt ggggataaaa
     4201 aaataacagg tgaaatgtta agaaagagaa tggagccatt attttattta aatgcaaaaa
     4261 ctactatgac tgatgacgaa atacgttcat atgaatcaaa tatgattgaa actatgatta
     4321 catctgatat attatcctct gaagcagcaa aagagaaaat aagtataact aatgaccaac
     4381 tagatgctca gtataataat ttaatgagta gtatagaatc agcaatggga atgactaaag
     4441 aagagttttt aaaacagttt gatttgactg aaaaatatat aaaagatgat atgaaaaaag
     4501 agctagtagc atcaaaatat ttaaatgaaa aatcaaaagt tactgataaa gaagcagaga
     4561 actattacaa taaaaacaag agcaattatt tacaagttag agcttcacat attttaataa
     4621 aaactgttga tgataaaggt aagcaagtta gtagtagcaa aaaagctgaa cttaagaaag
     4681 aagctgaaga aatacttaaa aaagcccaag ctggggaaga ttttgctact ctagctaaaa
     4741 aatattctga agatagtagt gcagaatcag gtggagattt aggtttcttt ggtaaaggtc
     4801 agatggtgga atcttttgaa aaagcagcct ttgcattgaa gaagggagag gtatctaaca
     4861 aacttgtaga aagtgattat ggatatcata taattaagaa aactgatgaa aaatatcaac
     4921 catttgaaga ggtaaaatca gatttagtaa gcagtttaac atcagaaaaa caaaacctac
     4981 ttatacaaaa tttaaaagaa aaatataatg ttaaaattac agataaatat caaatgaaag
     5041 ctaaagaata gataaagtca atttattaaa aaaaagaaaa attaactgta tggataaaag
     5101 ctatttttac tctcaatata gagagtctaa agt
//
LOCUS       DACTGW010000235         5125 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.238, whole genome shotgun sequence.
ACCESSION   DACTGW010000235 DACTGW010000000
VERSION     DACTGW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.238"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            184..1896
                     /gene="ptsP"
                     /locus_tag="I9573_003191"
     CDS             184..1896
                     /gene="ptsP"
                     /locus_tag="I9573_003191"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAT4797786.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASIHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            complement(2688..3353)
                     /locus_tag="I9573_003192"
     CDS             complement(2688..3353)
                     /locus_tag="I9573_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797787.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            3697..>5125
                     /locus_tag="I9573_003193"
     CDS             3697..>5125
                     /locus_tag="I9573_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HAT4797788.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKALKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEDQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT         2121 a          515 c          849 g         1640 t
ORIGIN      
        1 tataaatatt ttagaaaatg aaataagata tttttaatca taaattcaga gtaaaaatac
       61 agtaacaaac agtttataaa atttaataaa aaattataag taaataaaac tataaaaatg
      121 taaattattt atataaattt atagagttta tgtgctagat gatatttttt aggaggtagg
      181 gttatggctt acaaaggaat aggtgcttct cctggtgttg ctttagggaa agcgttagta
      241 gttgaacata gtgaattagt aattgaaaaa aaatctatag ataatgttga agctgaaatt
      301 gctaaattag aagatgcagt tgcagtttct aaagaagaat tagtaaaagt taaggaaaag
      361 gcttctgaag aattaggagc agaggaagca gaaatatttg aagcacattt attagtttta
      421 gaagacccag agttaattgg ttctgcaata gataaaatta aaactgaaag tgtaaatgca
      481 gagtatgctt taaatgaaat aaaagagatg tttgttagca tgtttgagtc tatggacaat
      541 gagtacatga aagaaagagc ggcagatata aaagatgtta caaatagaat acttagacat
      601 atattaggaa ttaaagttgt tgatttatct gctttaagcg aagaagttgt attaatagca
      661 catgatttaa ctccatcaga cacagcaact atgaataaaa aaatggtttt aggattttta
      721 actgatatag gtggaagaac ttctcatact gctatcatgt ctagaacttt agaaatagct
      781 gcaatcgttg gattaaatga tataacttca aaagttaagg atggagattt tgtagttttc
      841 aatggagaca caggtgaagt aattgtgaat cctgatgaag agactataaa taaatatact
      901 gaattaaaag ctaagtatga agatgagaga aaagcattac agctattaaa aggaaaacca
      961 tctgtaactt tagatggaaa acatgtggaa ttagctggta acataggaac acctaacgat
     1021 atagaaggtc taataaagaa tgatgctgaa ggtgttggtc tttatagaac agaattttta
     1081 tacatggata gagattcttt cccaacagaa gaaatacaat atgaagcata caaagctgta
     1141 ttagaaggaa tggatggaaa gcctatagtt ataagaacat tggatatagg tggagataaa
     1201 gaattaagtt atttaagtat ggaacctgaa atgaatccat tcttaggtta cagagcaata
     1261 agactttgcc tagatagaaa agatatattt aagactcaat tgagagcttt atatagagct
     1321 agtattcatg gaagattaag aatcatgttc ccaatgattt catctttaga agaattgtta
     1381 caagctaaag aagttgtaaa agaagtttta gcagaacttg attctgaagg tgtggcttat
     1441 gctaaagatg tagaaattgg tatgatgata gaagttccat ctgctgctgt aatatctgat
     1501 gtattggcta aacacgtaga tttcttctct ataggaacta atgatttaat tcaatacact
     1561 tgtgcagttg atagaatgaa tcaaaagata agttatcttt acaatcaatt taatccagct
     1621 gttttaagac ttataaagac tgttatagac aatgcacata aagaaggaaa atgggcaggt
     1681 atgtgtggag aatctgccgg agaccaaaaa atgataccaa tacttttagg tatgggatta
     1741 gacgagttct ctatgtctcc aatatcaata cttcctgcta gaaagttaat aacttcagtt
     1801 aaagagtcag atatgaaaaa attagctgat gatgtactta atatgggtac tgctgaagaa
     1861 ataaaaagct atatagaaaa gacttttaat atataattaa tgttatatag ttactaaaat
     1921 tcatttttct aaataacaaa attatatttt acgaattaaa aaggagatgt ttcaaaataa
     1981 aaatttgaag catctctttt ttataaaaga ttactaagaa ttacttaatc tttgtaatag
     2041 tcattttgtg aaaaagcaaa actgcattgt gactatagta tatatggatg ttattatcaa
     2101 taaaagtact attaaaatga agattcattc tcaaatgata tcaaaagtag ttgtgatgga
     2161 gtaagataat attttgtgat ttaatagtaa aaattaaaaa aatatggtat tgaaaaataa
     2221 ttaaaagtat ttaataatag aattagggaa gaataagttt ataagtaaaa ataattgatt
     2281 actagttaat aaaataaatg tgatatccaa ataaatccta aatctaattt gagttttaat
     2341 atagatagtc taaagtttga ttattaaaga tttaattgta agttgttaca gttgatgata
     2401 ttgattagag attaaagttt agagtagaaa gttaccttaa atttaaaaat gcaagataac
     2461 ccaatctaaa aattataatc actcgatagg tttaaggatt tatttgttta ttataaatag
     2521 gagggtgtct caaaatgaac tttttagttt atgaggcact ctcttttata tagaattaaa
     2581 tatattttat tatttcaaca atgaaaaaga agcttatctc tattttgaga cagcctcttt
     2641 gtatactatt attaatttaa ttaataatat aatttaaaat tataatatta gttaagattt
     2701 cctgctttat tagtaaataa ttctttaata gcattgtttc tggaaactaa atcgttatca
     2761 gagttgtttg tttttttcat aatcactgaa taacgttttt tatttataaa aaatattaaa
     2821 atcttatcat catcaaaatt gtattcttta tatctgtcaa tagactcata acctagattt
     2881 agaatgtttt tgtcttttat actatctaca tctaaaatat atgatgggtc agcctttagt
     2941 aatatattaa ttaaacattt ttcttcacca gcacagaata actccatctt acttgatata
     3001 gatttagatt gctctgaagt tatttttgca ggaataaggt tgtcattatc atctcttaga
     3061 agataaccat gtgtgtcttg ttgatatact aaattaacct ttttcttagg aacactaatc
     3121 attttatcta aatgtacata atgatgattt tcaacaaaac cttcatttaa tatttcaaca
     3181 aagttattaa ttttacctag gcaaaaagat tctatagatt ctttacagaa tttatttgta
     3241 aatggtatta tgtttacgtt ttcctcatct gtattccatg ctatagcata atccaacgaa
     3301 tatacttctt cctctatatc tgcaaaatta tattgtacta tgtttccttt caaaattact
     3361 cgacctccat cttataatat atttaatata ctttataaaa cttatattat atttaaataa
     3421 tatcataaat tggataaata tgatatagta aataagaaaa aatttgaaaa aaatcactat
     3481 aaatgagtaa taaatataaa tagtgtatac taatgggatg gaaaatgtcc actattaact
     3541 ttattacccc acatatataa gataaaatta ttaatataaa atataatcta tcgacaatga
     3601 atagactttt aaataaactt aatataaaat taattaaata agtgcttaaa aacgatttac
     3661 tttaatgata gaatcatatt aaaggaggat aattttatgg atataaagaa taaaattaaa
     3721 tctatatttt taccagaaaa aaatgactat ttagatgatg aagaatttga gtatgatata
     3781 gaaaataatg aagaagattt aaaggaagat aacaaagtac atttaaatga tttaagcaga
     3841 gtaaaatatg attacagaga gttaaaagac atagaaaacc aaacagaaga gatgtgtctg
     3901 ctagcagtaa agcaggatgg tactataatt gagtttgtaa aggacaagac ttataaagta
     3961 tgtatggaag cagtaaaaca gacatataaa gctcttaagt atataacaaa tcaaaatgag
     4021 gatatttgta tagaagcagt aaaacagaac tacagagctt tgtactacat aaataataag
     4081 acagaaaatg tactaattga ggctataaaa aatgcatcaa cttatgatgt catggaagta
     4141 tttaaatttg tggaagatca aacagaagat gtatgtctag catttataga aagggcatct
     4201 aaaaatgatg ttgcagagat attaaaagga ataaaagagc aaactccagc catatgttta
     4261 gaagctgtaa aaaaagatgg aaaatcttta gcttatgtaa aggaacaaag taatagtata
     4321 tgtttagaag ctgtaaaaga aaactacagt gcactatctt gtgtaaaaga gcaaacagaa
     4381 gaaatatgta tagaagcagt aaaacagaat gactttgcat tgtattatgt aaatgaacag
     4441 acagaaaaaa tatgtatgga agcagtaaaa agaagttata tggctctaca gtatgtaaat
     4501 aagcaaacag aagagatttg tttggaagca gtaagaattg atggaagagc attacagtac
     4561 gtaaaagagc aaacagaaga aatttgcttg gagtcagtta gacaaaatgg aaaagtattg
     4621 caatatgtaa aaaaacagac tgaaaatata tgtatagaag cagtaagagg ttcatttgaa
     4681 gaacttgaaa taaaagaaat attaagttat gtaaagatac caacagaaag aatatttgta
     4741 gaagcagtaa agcaaaatgg aaaaattctt aaatatgtag aaaatcagac agaattaata
     4801 tgtttagagg cagtaagaga aaactataat gctttagctt atgtaaagga acaaacagaa
     4861 gagatttgtt tagaagcagt taatcaaagt tatgaagcgc ttaaatatgt gaaagaacaa
     4921 acagaagaag tttgtctaaa agcagtaaaa caagattata gaatgttgaa atatgtaaat
     4981 aatcaaacag aaaaaatatg tttagaagca gtaaagcaaa attatagagc tttagaattt
     5041 gttgataatc aaacagaaaa agtttgtctt gaagcagtaa aacagaatag aaaagcacta
     5101 cagtatgtaa aacaaaaaca aagtt
//
LOCUS       DACTGW010000236         5090 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.239, whole genome shotgun sequence.
ACCESSION   DACTGW010000236 DACTGW010000000
VERSION     DACTGW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.239"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            232..1218
                     /locus_tag="I9573_003194"
     CDS             232..1218
                     /locus_tag="I9573_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAT4797789.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPMVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1441..1524
                     /locus_tag="I9573_003195"
                     /pseudo
     CDS             1441..1524
                     /locus_tag="I9573_003195"
                     /inference="COORDINATES: protein motif:HMM:NF012587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1555..3036
                     /locus_tag="I9573_003196"
     CDS             1555..3036
                     /locus_tag="I9573_003196"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAT4797790.1"
                     /translation="MYKEPKYRTILESIKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSVLEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3062..4033
                     /locus_tag="I9573_003197"
     CDS             3062..4033
                     /locus_tag="I9573_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HAT4797791.1"
                     /translation="MKKVISIGEALIDFIPNQNGCKLKNVSEFRRVAGGAPANVSVVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFMTGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4289..4891)
                     /locus_tag="I9573_003198"
     CDS             complement(4289..4891)
                     /locus_tag="I9573_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT4797792.1"
                     /translation="MEICILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEKHSRQFAKQIYYILDNSIEVDNC"
BASE COUNT         2154 a          518 c          820 g         1598 t
ORIGIN      
        1 ttataaataa attagagtat ttatcaattc atagtagata aaattgaaat taaatataac
       61 aacaaaaaat gaaaattaca attaatttaa tgttaaagac tcaattattt ggatgtggaa
      121 tggatagtac aagtagtata taaaaagtat tgactggaca aataactata ttttatttta
      181 aatatataat ataatgtaat aaaatatatc aaatgtagaa ggtgataacc tatgaaaaaa
      241 ataacaataa atgatattgc taacttggca ggagtatcta aaagtactgt atcaagatat
      301 ctaaataata aagatatcag tgattctact aaagagaaaa taaagacaat aatagatgaa
      361 tatggatatg agccgaatgc atttgctcaa agtttgagag ctaaaaaaac atactttatt
      421 ggtattataa caccgtgttt agattctttt gtaaaatcta aaattatgat ggcaatagat
      481 gaagaactca aagaattaaa atatacatca ctaattatta atactaatag aaaaataagg
      541 tcagaaatag atagtatatc aaagttagct agtctaaaag ttgatggaat aatactaatt
      601 ggtaccgaaa taactaaaga acataagaat gaaattgaaa agttagatat accaatggtt
      661 gtagttggac aaaaagttga tggtatcaat agcatagtga atgatgatta tggagcagga
      721 tataaaatgg gtcaatatat tgctaataaa ggttacaaaa acattgtata tttaggtgtt
      781 gatgaaagtg atatatcagt tggtttaaat agaaaaaatg gagtacttaa tgggttaaaa
      841 gataaagggt atgatgcaaa agtattttat actgactttg accaagaaac gtctatacaa
      901 agaagtggag aaatgctaga aagtgaaaat ccagatataa taatatgtgc aactgataat
      961 atagcaatag caacaatgaa agaaatcaat aagagaggta aaaatatacc acaagatata
     1021 tctgtagctg gatttggggg atatgatgta ttgtctatta taagccctaa acttacaaca
     1081 ataaagtttg aaaataaaaa tgcaggtaaa gtagcagcaa atacgatagt gaacttaata
     1141 caagaaagaa aagaaccact attaaaggag attaaatttg aattaataga aggtgaaagt
     1201 acaataaata aaaattaaga tacacattac atatattagt aatgtgtatt tttttactaa
     1261 aaaaactata attctaaaaa ttaggaaaac ttttttgaaa gaaatattgc atctgaaata
     1321 aatttatgct aatctttaat taaaccggtt tcgaaaccgg tttaattaaa ccgaaagaat
     1381 agcacaatga gtgcaaaaat aactgattgt aacaaagtat caaagtaatt attagagtac
     1441 ataggaaata aacaaaatat aaaaggtgta gcacattgtg taacaagatt aagaattgtt
     1501 ttagatagta ataataaagc aaatataaaa ttacaaaaga aaaggaataa aaatatgtac
     1561 aaagagccta aatatagaac catattagaa tcgataaaag aagaattaaa taaattaaga
     1621 aatatatctt taaatgataa atataaacca ctatttcata tacatccaca acatggacta
     1681 cttaatgatc caaatggatt agcatattat aatggtaagt accatgtatt ttatcaatgg
     1741 tatccatatg atgcaactca tggaatgaag cattgggctt atgtaagttc tgatgatttt
     1801 gtaaactgga atagagaaga tgtagcatta ataccaatcg aaagttatga atctcatgga
     1861 gcatattcag gtaatgcgat agaggtagat ggaaaattgc atatgtatta tacagggaat
     1921 ataaaatata gtgcagaaga tagatatgca tatcaaaact tagctataat gaacaaggat
     1981 ggtaaaataa ctaaatatga aaataatcca atagtaagtg aaataccaaa aggatataca
     2041 ggacatgtaa gagacccaaa agtatttaaa agaaaggata aatactttat gttacttgga
     2101 gcacaaacaa gtgataaaaa aggagttata atagtatatg aatctaaaaa ctctatagat
     2161 tggaatttta aaggagaatt aaatgtaaaa aatatagatg aagattttgg ttacatgtgg
     2221 gaatgtccag attatattaa tatagatgaa aaagatatat taatattttc gccacaagga
     2281 gtagaaccta aaggatttga ttatcaaaat atatataatg tagtatatgc tattggtaat
     2341 atggatttag ataatttaac atttgaaata gatacaatga aagaactgga aaaaggattt
     2401 gatttctatg ctccacaaac atttataaag gactcacaaa taatactatt tgcatgggct
     2461 ggaatgggag aagttttata ccctacagat aaaaacaaat gggcacattg tctaactgtt
     2521 ccaagaaaat taaatataaa aaataacaag ctattgcaaa tgcctgtaga tgaattaatt
     2581 aaattaagat atgatgaaac aagtgggcaa aatactataa agaataatat caatattata
     2641 gaaaatgatg aaaatgtata tgagcttaat attaatataa aaaatattga cagcaataaa
     2701 tttgggctgg aattattttc atcacaagat gaaggtgtaa aattagaatt taacaaatta
     2761 ggaaacattg taactttaga cagaagcaac tttaaaaaag ttttcggtgt tgaatatgga
     2821 acaaatagaa aagaatatat aaatattgat gaaaatacta atatcaaagt actagcggat
     2881 agaagtgttt tagaaatttt tataaatgat ggagaagtag tatttactag tagaatattt
     2941 gctaaagaaa attctaatca aataagggtt tatagtgata aaatagtaga gtatgagtat
     3001 actaaattta aattaaagca aggtattgaa ttataaaaac acattataag gggtagaaaa
     3061 gatgaaaaaa gtaattagta taggagaagc tttaatagac tttataccta atcaaaatgg
     3121 ttgtaaatta aaaaatgtat cagaatttag aagagtagca ggaggagctc cagcaaatgt
     3181 tagtgttgtt gtagcaaagc ttggaggaaa atcaagtttt atatctaagt taggaaaaga
     3241 tgcatttggt gattatataa tagatgtatt aaatgaagta aatgtaaata ctgattatgt
     3301 attgagaaca agtaaagcaa atacaggact tgcttttgta tcattaaaag aagatggtaa
     3361 tcgtgatttc tcattttata gaaatccaag tgcagatatg cttttagaag ctgatgaggt
     3421 taaaaaagag tggtttaata attgtcatat cttacatttt tgttcagtag atttaataga
     3481 tagtccaatg aaattagcac ataaaaaagc aatagaatac gcacttgaaa gtaatagtat
     3541 aataagtttt gaccctaata taagattacc tttatgggat agtgagcaat cttgtaaaaa
     3601 ggcaatatca gaatttttac cttttgctca tattgttaag atatctgatg aagaattaga
     3661 atttatgaca ggagaaaatg atatagaaaa atcattacat aaactctttg tggggaatgt
     3721 tgaactagta ttatatacaa aaggaaaaga tggagtagat gcatacacta aaaaagtaaa
     3781 aggtatgtgt aaaggggtta aagtaaatgc aatagataca accggagcag gagattctta
     3841 tataggttca tttttataca ccttattgta taaacaaata acattgaatg atataaaaga
     3901 aatgaaacaa gaaacgttaa atgagtattt agaattttct aattattatg cagctcgtag
     3961 tacaactatt aaaggtgcga taagttcata tgcaacaaag gaagagataa ctgaatttat
     4021 aaaaaattta tagtaggaat gagtaatagg ttttgaagga agtaacatgt aaagagtttt
     4081 taaggatgtt tactatgaaa ataattaaca tataaaaagc aataaagagg ctgtctcaaa
     4141 atagatataa gcctctttat cattgttgaa atgataaaaa atattttaat tattgttact
     4201 ttttagaaga atatatatct tattttaaat aagcaaatta tgtaagattt atttatatta
     4261 tcaatacttt acttggagta ttatgtcttt agcagttatc tacttcaatt gaattatcta
     4321 aaatataata aatttgtttt gcaaattgac gagaatgttt ttctttttct ttattcataa
     4381 aattaggttt acctaaatca cgttcaacaa gcattcctat atattttaac tgtgaatgct
     4441 tacagtagcg cttcatacca ttttcccaaa tatcagcacc cttttcaggt ctatatccac
     4501 atgttgtaat aagtgctact ggttttcctg cccatagagc ggggcctttc tcattaccat
     4561 agtacttatt cattccatac accaatctgt caagtagtgc tttcattgga gaagtgcagt
     4621 accaagaata aattggagta gcaaaaacaa ttaaatcaca cataagaatt ttcttgaaaa
     4681 ttttctgaac atcatcttta caataacatc caaaatcaaa taagttatcc tgacattttt
     4741 tacaagcaat acatggctct attttacaat catataacca tattacttca atatttgaat
     4801 tataattttc taattcctca ataaaaggtt ttaaaatagc agcagtatta ccttttttct
     4861 taggacttcc cataagaatg catatttcca tattgatacc tccacagtat tttaaaatag
     4921 tttttagtgc ttaatatagt tttaggatac tatgagaata cgttaacaac aatacacttt
     4981 gtatattttt aataaaattg aaacaaattg ttttatcaat aaaagttgga atgtatctat
     5041 aattaaaaat attttaggta tgtttttaca tatatgtgtg tagttaacta
//
LOCUS       DACTGW010000237         5076 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.240, whole genome shotgun sequence.
ACCESSION   DACTGW010000237 DACTGW010000000
VERSION     DACTGW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.240"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            285..974
                     /locus_tag="I9573_003199"
     CDS             285..974
                     /locus_tag="I9573_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4797793.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYFVKTAVDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            974..1891
                     /locus_tag="I9573_003200"
     CDS             974..1891
                     /locus_tag="I9573_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4797794.1"
                     /translation="MELFLVVISIVLFAFYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATLEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            1964..2890
                     /locus_tag="I9573_003201"
     CDS             1964..2890
                     /locus_tag="I9573_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797795.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNDVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     KNQIGINSIGRKQSSLEDYFFQTTGTGGIETL"
     gene            2887..3567
                     /locus_tag="I9573_003202"
     CDS             2887..3567
                     /locus_tag="I9573_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAT4797796.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSILGILLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLVFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            4012..4713
                     /locus_tag="I9573_003203"
     CDS             4012..4713
                     /locus_tag="I9573_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HAT4797797.1"
                     /translation="MNNSFLDKLIETKELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGRHTLDDVQETVNSSYVFDTVIDSLKEKITHNFLRNLHSSLIFNTTLH
                     SRGMAGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKMLWK"
BASE COUNT         1953 a          537 c          794 g         1792 t
ORIGIN      
        1 cttttttatg tataaaatat tttttagcaa aagttaaaag gtgttttata atataaaata
       61 gttaattgta gatgttaata aaagaaaatt atgtatattt cagataaaga taagagagta
      121 tactatattt taattatttt ttcaataaaa cttattgaaa agaactttaa cttagaagag
      181 tatcaccaaa ttgcagatac aatatcataa gcagttattt attgacaacc ctttttaata
      241 aatatatcat ttgaagtata ctgacgaaaa ggagttgaat aataatggac atcttattgg
      301 ttgaagatga taagaatatt cgagaaatgg tatacgattt tctgattagt gagaattatt
      361 ttgttaaaac tgccgttgac ggaaatacag ctattgaaat atttaaaaat tattcatttg
      421 atattatttt actggacttg atgttaccaa agtctagtgg ttttgaagta ttaaaatata
      481 tacgaagtgt gagtgttgtt cctgttatta ttactactgc gaaagatagt gacacagata
      541 aaatgctagg gcttaatctt ggtgcagatg attatattac aaagcctttt tcaatcgtag
      601 aattgcttgc aagaattaaa gctaatatta gacgtgcaac ggtgtatagc agttcaagaa
      661 actctgaaga tatggttatt cagtataaag atttgattat aaacatatca aaatatgtag
      721 tttcaaagaa tggaattgac ttaaacttaa cacatacaga atttgaaatt gttaaattac
      781 tagcgactaa tcaaggacgt gctttttcaa aggaacaatt atacaacact atttggaaag
      841 aaccttatta tggaaatgaa aatgtgctaa atactcatat taacaggtta agaaataaat
      901 tgtctgatgg tatagaaaaa aataattata ttaaaactct ttggggaatt ggctacaaaa
      961 tggaggaaga ataatggagt tatttttagt tgtaatttcg attgtactat ttgctttcta
     1021 cttaaaagaa cgaaataagt caaagaaaat ggaaagtgat tataagtata tcaattcaag
     1081 acttcgtgat ataacaaatg atgatgaaat taattatatt ttagtaccat ctgatatttc
     1141 gattgtaaaa gaaacagcag tagatataaa taaacttctt gaaaagtttt acagtaggca
     1201 aattaattat aatagaagtg aaaaagcgat attgcaaata tttacaaaca tatcacacga
     1261 tttacgaact cccatcacag tattaaaggg ttatattgaa atgctatatc ttcaaagtca
     1321 aaaagaagaa ttatcttctt caatgagagc aactttggaa aaaatgcaaa caaattcaaa
     1381 tgagcttgtt aattcaataa ataacctgtt taatatggca aaaatccaat ctggagatat
     1441 gatattaaat cttcaaaaat ccaatctaac acaattatgt catgagatta ttttagagtt
     1501 ttatgattta ctagataaag aaaactttaa tgttgaagtt aatattgaag atagacctat
     1561 atatgcaaat gttgacacag aagcattaca acgtatactc aaaaatttaa ttgataatgc
     1621 tataaaatat ggttcagatg gtagattttt agggatttca ctatatgaaa aggaaaaaca
     1681 tgtttttata gatattgagg ataaaggttc aggtatttct gaaactgaaa aagagcatat
     1741 tttcacaaga gcttatactg ctgacagaaa aaaaggcaat ggtttaggtt tggctatttc
     1801 tcaagggctt gcaaactcta tgggaggttc aatttatgta aatagtagac ctaatgtaaa
     1861 aacagttttt acggtaaaat tgaaaagtta gaaaaaagtt agatttaaaa gagagaaaag
     1921 ataattttat tttgtatact gtttttagaa aggagggagt atgatgaact atatcataga
     1981 gacccatcaa ttaaagaaaa tatataaaga taaagctgtt gttaatgatg ttaatattca
     2041 tgtgaaaaag ggagaaattt atggatttgt tggaccaaat ggagcaggta aaagtacagt
     2101 aatgaaaatg ttgctaaatc tcgttaagcc aaattctgga gaaatagtta tgttcagtaa
     2161 gaaagtggca gaaactgatt ttgaaatatt aaaaaagatt ggtactatta ttgaaaatcc
     2221 ttatttctat gagaatttga ctgctaaaca gaatcttgat ttacactgtg aatatatggg
     2281 ttattataac aaagaacata tatcagaggt attggaatgt gtcggattgt caaagcaatc
     2341 aaacaagaaa gtttcaaaat attctttagg gatgaaacaa cgtcttgcaa tagctagagc
     2401 aattcttact aaaccagaat tattgattct tgatgaacct ataaatgcac tcgacccaga
     2461 ggggataaga gaaatgcgag aattattccg taagttaaat acagattatg gaataactat
     2521 atttatttct agtcatattc tttcagaggt agaacaaatt gcggacacta ttggaattat
     2581 tcaagatggt aaacttatta aagaaatttc tatgtcagat atacacaaat atcaaacaga
     2641 ttatgttgaa gtagatgtag ataatgtgga acttgctgga tacttgttag aaaaagaatt
     2701 tggaataaaa gactttaaaa ttacttcgga aagttgtatt gaaatatatg atttacgaaa
     2761 agatgtaaaa gaaatatcca aaatatttat caaaaatcaa attggtataa atagcattgg
     2821 acgtaaacaa agttctttag aagattactt cttccaaact acaggtacag gaggtataga
     2881 aacactatga tacatttaat gaaactagaa ttaaaaaaag ttgggctaaa aagatatttt
     2941 ttattttcaa tactggggat tttgttatgt atgttttttg tctttactgg attgaatgat
     3001 aaatctactt caatatatga ttatgatgtt acatttagga caatcagttt agttttctgt
     3061 ttttactata ttatattatt tgctgtgttg aatgtggcat atataataaa tgaatataca
     3121 aataaaacaa tcttgatttt attctcttat ccacttgacc gtaaaaaact tattttatca
     3181 aagttacttt tgattacgac tcttatgata tcatcaatga tagttggata tatctgttgt
     3241 tttacattcg ttgttgtgtt ggataagtat tttaatttag ttattggaga ttttcatatt
     3301 tcaattttat cttattggat accaggagca attaaaagta ttattgtatt ttgctcatta
     3361 ggtatatgga cattcgttgt tggaatgata agaaaatcaa tacctgttac ccttgtcagt
     3421 tcaattattt ttatcaattt aagacaattt attttagctg gtacaaatac aactcaagac
     3481 agtctgattt ctgtttttat tatagttgga ataactgtga ttggtatata ttatatacta
     3541 aatcataaag ttactgaaat tgactagata ctgtggatag gaaggtagtt attaattgta
     3601 tcttcttggg ataataatga tttgctatta tatcagctat tattaatatt attagagttt
     3661 taataatatt aataatagct atatattttt tcttcttatt tataaattta atgatttata
     3721 gtcataaaat gaactctaag aagagataaa aatgcgtttt atatgttttt agattaatat
     3781 gaaatatcac aatgaaatgt tgtacaaagt gatatataat aattatatga actataaaat
     3841 tgactaaact tattgactaa taaattagat gaaaagttga gacttactga aaataaatac
     3901 aatgaaatat atactacaag tattaaatta aatctgaact ttacatacta ttagaataag
     3961 tattataaga ttataatgat ttaaaaatat taaaataatt gaggtgataa catgaacaat
     4021 tcctttttag ataaattgat tgagacaaaa gaacttaaaa attcacttta taatgtgtta
     4081 aaacacaatt ttctttatca ttctaataag atagagggca gtacttttac tactgaagca
     4141 ttagcacttt tactcgataa gaatgttgtt acaggaagac atactttaga tgatgtacaa
     4201 gaaactgtta attctagtta tgtatttgat acagtaattg actcactgaa agagaaaata
     4261 acacataatt tcttaaggaa tctacattca agtttaatct tcaatacaac cctacattcc
     4321 agagggatgg ctggtatata taaaactata cccaatatga tactaggaac tgatgtaagc
     4381 atagcacaac catttgaagt tgagccaaag cttgatgaat taattgaatg gtactatagt
     4441 cagagtgaag tgtctattaa agttatagct gaatttcatt atagatttga attgatacat
     4501 ccatttcaag atggtaatgg tcgcataggt agatttgtta tgctaaaaca gatgttagaa
     4561 aataatcttc ctataaaaat tgtatcttgg gatagtgagg acttgtatag aaactcctta
     4621 aattcttgta gtttaggtaa ttatgtccct ttaattgaat atttatcaag tttagaagat
     4681 tttagagaag tatataaaat gttatggaaa taattgtttg tagcaaaaaa agtttttata
     4741 taaatatatt tttaactatg ctatttaacg ttgatttctt agttattctt agcttggact
     4801 atacccacta ttttaactat ataaaatagt ggatatagtt taactatctc tttataacta
     4861 tttttatgta tcttagttgt ttcttaatat tgggacagtt cataaaacta aaagtgattt
     4921 ttaattttat catttataat ttaccattac atcagaaaaa tgcttatcat tagaattttc
     4981 ataaaaggta cctattttct tcaattaact aaatcaattc tattagtctt aatctttcgt
     5041 atttcaaatt gataatacaa gaaaataaaa aataaa
//
LOCUS       DACTGW010000238         4968 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.241, whole genome shotgun sequence.
ACCESSION   DACTGW010000238 DACTGW010000000
VERSION     DACTGW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.241"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..600)
                     /locus_tag="I9573_003204"
     CDS             complement(<1..600)
                     /locus_tag="I9573_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAT4797798.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIKDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSGLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLY"
     gene            complement(727..1416)
                     /locus_tag="I9573_003205"
     CDS             complement(727..1416)
                     /locus_tag="I9573_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT4797799.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLRVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMIGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(1424..1987)
                     /locus_tag="I9573_003206"
     CDS             complement(1424..1987)
                     /locus_tag="I9573_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAT4797800.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(1989..2957)
                     /locus_tag="I9573_003207"
     CDS             complement(1989..2957)
                     /locus_tag="I9573_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAT4797801.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(3058..3101)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3466..4095
                     /locus_tag="I9573_003208"
     CDS             3466..4095
                     /locus_tag="I9573_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT4797802.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQSY"
     gene            4070..4474
                     /locus_tag="I9573_003209"
     CDS             4070..4474
                     /locus_tag="I9573_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAT4797803.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            4477..4950
                     /locus_tag="I9573_003210"
     CDS             4477..4950
                     /locus_tag="I9573_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAT4797804.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
BASE COUNT         1806 a          787 c          551 g         1824 t
ORIGIN      
        1 atagagaaaa gtttcctctt cacatttaat aaaacaaatt gatagcaacc atgccatacc
       61 cattttaaca taatattctt cactttgtat attattacag tattcaaata tttctttaat
      121 atgtttatca tcaacataat atattaaaaa cataactaat gcaaatctaa tttcccatgg
      181 attctttgaa taaacatatt ttttaagtat atcatacata ttttctttat attttttcat
      241 agactttaaa ccagaacaaa acccatcgca aactgaccaa ttatcgattt taggtataaa
      301 tctttttaca tagtataaag tttcttcaaa attactattt atattgccaa ttaccatacc
      361 ttgtagccta acttcttcat agtatgtatc ctctgctact tctaaaaatt ccttccaatt
      421 cccttggctt atttcttttg ataactttct taaattagga actctaatac ctattatatt
      481 ttctgttcca ggtataagct ttttattaaa atctctatat tttatatctt ttatagacat
      541 catatattct aaaagttcat tataatccaa tttattccat tttcccctat caaacatcat
      601 ttaattgctc cttaattcct tttttcttat tgtaacataa aactctacaa aaaaagcttc
      661 taactattag aagctttttc aaaaacataa gaaacttttc aattttaaat ttaaactaaa
      721 ttaatgctaa tctcctacac caaagtctgt attattgtat aaaacttcta tatttggatt
      781 ttcatccaag aatttatact tactaacaaa ccatttcact aactcttcat ctctttttat
      841 actagaagaa ttatttataa aaacattcac tgtcgctttt tcaaaagtat ttaggataat
      901 ctctatatct ttatctatca tctcttttgt ttgtcctttt attccaatca taatacaagg
      961 tgaatcaaaa tattctttta catcttgtgg agtcttaaaa tttgcatttt tatttaaaat
     1021 attatttcta aaatcattgt caaaggtttc aactccaatt ttaaatacca caggaatttc
     1081 aaaatactct ctcatttcct taagcctatt tttgtaagac caatgacttt ctaaaaataa
     1141 tttttttata ttcttctctt ttataataca ctttattttt tctaaagtat ccttaggtaa
     1201 ttcaaaacag cttccagaat ttatgacttc aagaactcca tatttcccag ttacattttt
     1261 taaaaccctt agatttaatt tattcatctc ttcttctaaa ttagaattat catctatata
     1321 atcacaaaaa gaacactttc cccatataca aggaaatcct ttaagcagaa caatttctct
     1381 ttgattttta tttgttatct cactatatct atccattttc tatctatttt cctccagtca
     1441 atgatacctt taaagttttt ggaactgcat ttaatcctaa attcactaaa acaactgcta
     1501 acagcttatc tccataatca gtaaatactt gggttacaaa cacactaaaa acatctccca
     1561 gtcctaacac tttcaagatt tgcactatat aggatgaacc tgaagaggtc atacctccaa
     1621 ataaaaatgc tgaaattatt gaacttatta tagatgttgg aatagcaaat aaaagtactc
     1681 ctagtggagt ttttaaaccc cttaaaaaat tctttttaaa tgctattcca gcaagtaatc
     1741 cggttgatat ttgaactggt gcaaaataga gagaatacac atcaaacgta attccactta
     1801 ttaaacttcc aaacacacct gtaacaacag cgtatttagg tcctaaaagt gaagctatca
     1861 ttatagttcc tattgaatct aagtatacag gtaatcttaa tcccatagct ataaaagcgc
     1921 ctattatatt tatagaaata ccaaaagcta taagtgtaat agtttttaca catatttttc
     1981 ttttcacact atatcactcc ttccacagag tctataatgt cttcataacc tttaaaaagc
     2041 ctctttaaaa acatgtacat aaatttcttc tcatcaactt catttagaac aattgcatta
     2101 ggattttttt tatagaaatt gaaagaatcc actatagact gacccatagc tattccatct
     2161 tctacaactt ctacatatga ttcaaatcct ttacatatac ttctgtctat aaagtacgct
     2221 actgctagag ggtcatttat cacacagcca attattcctt cctgttccca atggaaatca
     2281 atataaaatc tagttatttc agttatatac tttgccatct tcttatcaag tctatttata
     2341 aactcaataa tattaggagt aagtacaatt tttctagtta catctaaacc taccatgtgg
     2401 atttttttag ataaattctt gtaaacataa tctgctccat gtgggtctac ccaataatta
     2461 aactctgcta ctggagagca atttccatga atcctaaatg ctcctcccat agatacaaac
     2521 tcatcgagat tttcaaatgc tttcttatct ttaataagtg ctttagctat atttgtaagt
     2581 ggtgcaagtg ctattattga tactttttca tgattatgta aagtatttat tataaaatcc
     2641 actgctccat tttttgcttt agctcctaca accttttgat aaaagttttc tccaatacca
     2701 tcttctccat gtgtatcttg agctgttaca agttttcttt ttagtggtgc ttcttctcct
     2761 atatatactg gaatatttag tgaagaacac atttgaagtg tttttagtgc attttctgca
     2821 cctatatttg ctggaacatt tccacaacat gtggtaattc caattacttc tagctctggt
     2881 gagtttaaag ccagaagaat tgccaaagaa tcatcaattc ctgggtcaca atcaattatt
     2941 acttttctct tttccatttt ttatcctcct tgatttgtgg tcgaggagaa ctacacatct
     3001 tttttccact ttcaaaaagg tgtgtctaag ggtattaatt atatactgcc actatataat
     3061 tccttagttt tttatacttg gagttctcga acaagtacag atgtaaaatc atatttttag
     3121 gctacttact atagactctt aaaaactata gactgttaaa aaatttttaa acatctgatt
     3181 taatagctat aaaataaatt ttatagcata aaagaattta ttatatttta atgtatctta
     3241 ttaaagttta tttagaatta tgaaccaact ttaataagat gtggcgcatt taaaccgatt
     3301 attattatac aataatatct taaatattac aattaataac attaatttat tatccataaa
     3361 aatatattaa cttatattat ttaataattt attgacagta ttaaaattaa attatacaat
     3421 aacagtgtaa attaacttaa caccgttaag caaagaggtg attatatgag tatcaaagaa
     3481 agacgtaaaa atgaaaaaga agaaatgaag aaaaagatta tggatgctag catcgaaatt
     3541 ataaaccaac atggatatga aaatttatca ataagaaaaa ttgctacaaa aatagaatat
     3601 tctccaacta caatttatct ttactacaag gacaaagcag agattatcag cgatatgacc
     3661 aataaacttt ataacacagt agaaagtaat gctattgaca ttatgaataa ttgctcatca
     3721 ctaccaatag ataaacaaat catagaaatt atgactagct ttataaaaac tctttctagt
     3781 gaaccagaga tggcaaaagc tatcatgcat agtagaatga atattatatt tgcatctgaa
     3841 aatacaaaca atactcctag caataatggt ataaaaatgc ttgataaatt cctttccatt
     3901 ggaattgaac aaggaatatt caaaaaaaat atagataaaa gctcatggat gcttataagt
     3961 gcattgttag gattcgtttt atgtgttgtt gaaaataaac tatattcttt acataatttt
     4021 tctcaactta tagaaaattt cttagatatt ttagtgggag gactttacaa tgaaaattcg
     4081 caacaaagct actaataaaa agaaaaaata tgtaaaaata tctattgctc tattctttgt
     4141 atttatatgc attattattg taattgtaag caacttaaag ccagaaaact tggtagtaaa
     4201 agatatagat attaataatg ttaaaaatgg catttatact ggaagtgctg ataataactt
     4261 ggtaaaagct actgtgtctg ttgaagttaa taatggtaaa atccaaaata ttaatatctt
     4321 aaaacacgac catcttttag gtaaacctgc tgaaaaaatt acaacaagta ttattaaaca
     4381 acagtcttta gatgtggatg ctataacttc tgcaacttat agcagtaata ctatacgaaa
     4441 agctgtagaa aatgctttaa gaaagggaga ataattatga atactattat tatatattca
     4501 agtaaatatg gttgtacaaa agactgtgca aatatactaa aaaataaact atcagataat
     4561 gtcactattg ttgatattaa taataataat aaaattgagt tatcaaaatt tgataaaatt
     4621 attattggaa gttctatata tgttggttca gtttcaaaaa aaatacaagt gctatgtaat
     4681 gataatgtag agttattaaa caaaaaacaa gttggcattt tcctttgctg tggcttttct
     4741 gaacaagcag ataaatattt aaaatcaaat tttccatcat ccttattaga aagtgcaaat
     4801 gctattggaa tttttggtag tgaagctcga ttagaaaaaa tgaaattttt agataaactt
     4861 attatgaagg ctgtttcaaa aggtaattat gacagtttta gaatatcaca ggataatata
     4921 gataattttt taataaatct taatagctaa aataaaacta attatttc
//
LOCUS       DACTGW010000239         4873 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.242, whole genome shotgun sequence.
ACCESSION   DACTGW010000239 DACTGW010000000
VERSION     DACTGW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.242"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(31..1623)
                     /gene="hcp"
                     /locus_tag="I9573_003211"
                     /gene_synonym="priS"
     CDS             complement(31..1623)
                     /gene="hcp"
                     /locus_tag="I9573_003211"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAT4797805.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKLG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(1683..2408)
                     /locus_tag="I9573_003212"
     CDS             complement(1683..2408)
                     /locus_tag="I9573_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HAT4797806.1"
                     /translation="MMRKIIKIDEGKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            2549..3277
                     /locus_tag="I9573_003213"
     CDS             2549..3277
                     /locus_tag="I9573_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAT4797807.1"
                     /translation="MKTNYRLHMQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEM
                     LECLGVVDKTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLF
                     GEVFACTRLHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKN
                     ILLHNKIELLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRE
                     LGKMRDEGLLSFNKNKFEIFYFQK"
     gene            complement(3441..4712)
                     /locus_tag="I9573_003214"
                     /pseudo
     CDS             complement(3441..4712)
                     /locus_tag="I9573_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
BASE COUNT         1579 a          879 c          598 g         1817 t
ORIGIN      
        1 ataaatatct ttaagtatag atatatttat ttatcctaaa atcttcttca tatcttcttc
       61 aggagtagat ataggtgtca atgcaaattt atcaaccaat atatctaata cattcttaga
      121 taagaatgct ggcaaagaag gccctatata gatatttttg atacctaaat gaagtagtgt
      181 aagaagtata caaacagctt tctgctcata ccatgacagt accatgctaa gaggcaaatc
      241 atttactcca cattcaaaag catttgccaa tgaaactgcc actttaattg cactataagc
      301 atcattacat tgtcccatat ccattaaacg aggtaagcca cctatatccc caaggtctaa
      361 atcattaaat ctatattttc cacatgcaag tgtcaatata acagtatctt taggagcttg
      421 ttttacaaat tcagtgtaat aatttcttcc aagttttgca ccatcacaac ctccaaccaa
      481 taaaaagtgt cttatagctc cactctttac agcatctact actttatctt gtacagatga
      541 aactgtacct cttgcaaaac cagtagttaa tgtatctcct ccattaatac cagtcatatg
      601 aacatcttct ctatatcctc caagttcaag agctttttct attacactag aaaaatcttt
      661 atcttcatca atatgaacca tacctggata agcaactact tctgtagtaa atactctatc
      721 tctataacta tctttaacag gcattaaaca gtttgttgta aataatattg gagcaggaat
      781 attatcaaat tctttttgtt ggttttgcca tgctgtacca aaattacctt ttagatgttt
      841 atattctttt agttttggat atgcatgtgc tggtaataac tcaccatgag tatatatatt
      901 tataccctta tctttcgttt gttctaataa tttgtgtaaa tcataaaggt catgtcctgt
      961 aactacaata aatggccctt tctcgatttt catagatacc tgagtcggtt ctggtgttcc
     1021 ataagtttca gtatttgctc tatcaagcaa ttccatacat tttaagttta tttctccagt
     1081 tttcataact agacctaaaa gttcgtcaat agacaagtct cctccaactg ctattaatcc
     1141 ttcatagaaa aattcattta cgtctttgtc tgtataacca agtaccattg catgatatgc
     1201 ataggcagcc atgcctctta atccaaataa gattaatgat tttaatgaac gtatatcctc
     1261 attatcattc cataaatcct tcatatcata gttatcattt ctattacaag ttgttgaaca
     1321 atctgaacat ctaggtacaa gtttactttt ttctacctca gttttttcaa tttgtctttt
     1381 tatagcttca tcatcaaaac ttacattcgt tatagtagtg aatagcccct ctatcataat
     1441 tttatctgtt tcacttgttg gtctattctt atttccaata gttgctcttg aaagaccaat
     1501 caacactcct gtaagctcgt cttgcaagtt agctgtaccc ccctttttac cacaaacacc
     1561 atttatagta caacctttac ctcctgctgt ttgttcacat tgaaaacaaa acattttttc
     1621 cattttttat tcctcccttt ttatttatta aaatttatta tatttttcta tgattcactt
     1681 aattaaattt ctccatctac aatttttcca tctacagata tagtaactat ttgccatggt
     1741 atcatttttc cactattttg tagagctgtc ttaacagcat ttactatacc accacagcaa
     1801 ggaacttcca tacgtgttac tactatattt ttaatatcat tttcttttaa aatctctgtc
     1861 agtttttcag catagtcaac ttcatcaagt ttagggcatc cgattaatgt aaccttattt
     1921 ttcataaatt tattatgaaa atttccatat gcatacgctg tacagtctgc tgctattaaa
     1981 agacttgcat tctttaaata tgatgcatta gttggtacta attttatttg tactggccat
     2041 tgattaagtt gagaaccttt tatctctact atatcattgc ttatagatgt actatttgaa
     2101 acctctctat ttattgactt agactgagaa cctggacatc cacatgctaa agttttcttt
     2161 tgtgctttct ttgcttccat atttgctttt actgcagctt catcatattc tgctgcttct
     2221 ctatactcaa atgaaatcgc atttgttgga caagttggca gacaatctcc cagcccatca
     2281 caatagtcat ctcttaaaag ttttgctttt ccatttacca ttcctatagc ttcttcatga
     2341 caagcttcaa cacatagtcc acaaccatta cactttccct catcaatttt aattattttt
     2401 ctcatcattt atatcacctc ataaaatttt tttaatttaa agtattgatt tcttatatat
     2461 ttatttttaa tacctatatt tctttatttc aaatttcttg tagtaggatt tttatatccc
     2521 cttgctttta taaacttagt atagtacaat gaaaacaaat tatcggttgc atatgcaaca
     2581 aggaagtgat tatttgaaaa aatttttaga aaagcatata gacctactta aaaaaattcc
     2641 tctatttgaa ggaattaaaa gtgatgagct tgaagaaatg ttggaatgtt taggtgttgt
     2701 agataagact tacccaaaag atagtataat attctctgtt ggaagtgaag ttacttctat
     2761 tggaattatg cttaaaggaa gtgctcatat aataaaggaa gatatcgaag ggaataggaa
     2821 tattgtagct gaactcttgc ctggagattt atttggagag gtattcgcat gtactagact
     2881 acataaaagt aatgttacag taaccacaac atcttcttgc gaagttttat ttataaagtt
     2941 taaaagtgta actggtattt gttcatctgc atgtgtattt cataataggc ttgttgaaaa
     3001 tatgctacaa ttaatagctg aaaaaaacat tttacttcac aataaaatag aattactttc
     3061 tcgtaaaaca actcgcgaga aattgcttat gtatttttca aaacagatag aacaaacagg
     3121 tagccaccag tttacaattc ccttttcaag aaatgaaatg gctgattttt tatgtgttga
     3181 cagaagttct atgtcaagag agcttggcaa gatgcgagat gaaggcttgt tatcttttaa
     3241 taaaaataaa tttgaaatct tttactttca aaaataaact agatatttaa acatatttat
     3301 ttcaatatat actatattag ttaattcaaa ttataaatat catatttgaa tcttaaattt
     3361 atatttttac ttaaaaagtt aatagatatc taatttatta aaattaagat acctattaac
     3421 tttattaatt aagtgtacaa ttagcttatt ttctttgtag taattgctgt tttatctata
     3481 agccaatctt ttccttctac taatcttgaa actaacattg atgatactgt atttccagtt
     3541 gtatttaata tagtattagg aatatcagtt atagtagtta taactgttaa caatggaatt
     3601 gcctccactg gaaaaccgaa gatgctaacc acgagcatct caccaataag tcagccacct
     3661 gctacaggag ccataaatac tccaacaaca aatacagtta taaataattg acctatcgtt
     3721 gctggatttg ccattccact gcctaaaata cctgataaaa atgccacttt tattattact
     3781 gctaaaactg acccatcttt atgtatattt attccaagag gtataactgt ttctgatata
     3841 tcatcaggaa ctcctatatt tttactagct tctaaatttg atggtataca tgctgaactt
     3901 gaagaagtag ctagtgccgt tgctattggt tttatagcat ttctccagaa ttgtctaaac
     3961 ccttgttttc ctgcagataa aaaagcatac agactgtaaa ataataagaa atacatggct
     4021 gctgttagtg aataaattac aaacaccctt acatatcctt gaattatttt gcttccaagt
     4081 tccccaatta ttgtagctgt ataacagcct agccctatag gagcataaag catgactata
     4141 cttactactt ttaatattgc ttccgttcct tctgataaaa actttcttat tgtatttccc
     4201 ctatctccca tcatagctat tgctgttcca actaaaactg caaatataac taattgtagt
     4261 aaattgctac tggaaaataa atctccaaaa tttttaactg taatacttga tactacttgg
     4321 tctaataacg ttccttttga ttctaaagtt tctgcatctt gagccaccat taaacttttt
     4381 acacttgata catctaatcc tttagacgga ttaaataata atgcagatgt aatacctaaa
     4441 aatgcaatta caagtgttgt tcccataaat actatagctg tactcttcat tatttttcca
     4501 aaacgttcca tattttttat actagcaaca cctgaagaaa cactaagaaa aactaatggt
     4561 actactatca ttaatagagc atttatgaat aaatctccaa atggttttag tattgaagct
     4621 tttggaccaa gtactaatcc tactatcacc cctattatag tacatgctaa aagtataatt
     4681 gatgacttat aattttttaa aaagtccctc atttatattt tcctttctaa tgttttatat
     4741 tataaattac cctaaatcct aaaaaaatta aagtaaatta tctaatgcgc attaataatt
     4801 tttgtaattt tccccaacag ctactttagc aaatactgga atatatgtca atttctatag
     4861 tttttttgta tct
//
LOCUS       DACTGW010000240         4846 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.243, whole genome shotgun sequence.
ACCESSION   DACTGW010000240 DACTGW010000000
VERSION     DACTGW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.243"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            51..725
                     /locus_tag="I9573_003215"
     CDS             51..725
                     /locus_tag="I9573_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAT4797808.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKRKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            751..1587
                     /locus_tag="I9573_003216"
     CDS             751..1587
                     /locus_tag="I9573_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797809.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            1603..2274
                     /locus_tag="I9573_003217"
     CDS             1603..2274
                     /locus_tag="I9573_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4797810.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            2264..3496
                     /locus_tag="I9573_003218"
     CDS             2264..3496
                     /locus_tag="I9573_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4797811.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            3646..3963
                     /gene="trxA"
                     /locus_tag="I9573_003219"
     CDS             3646..3963
                     /gene="trxA"
                     /locus_tag="I9573_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAT4797812.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            3980..>4846
                     /locus_tag="I9573_003220"
     CDS             3980..>4846
                     /locus_tag="I9573_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAT4797813.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKL"
BASE COUNT         2082 a          439 c          858 g         1467 t
ORIGIN      
        1 aataagaagt atgaaaagaa agacaagaag atgttgacgg aggaatatta atgaataaca
       61 atgaagacag aattgacaaa tttttagaac ttcaaagcaa aggatttaaa tttgcagaga
      121 tagctcaaaa tctaaagatt agtcaaagta ctttaagaac ttttttaaat aaaaaaggat
      181 ataagtcaga aaaaggtaaa tacatattaa aagacgaatt aaattataat aaaagtttgg
      241 ataatagaaa cttggaagaa gagaaagcta aggagtatga aaaaagtaaa aagaatacta
      301 taagtaatag aaaaaaagta agcaccaaaa aaaagatttc taatgaaaag gctttaagta
      361 aaagtgttga acaaattgag tttaatgata caggtttaaa aaatgcaaag cgaaaacaaa
      421 atgttaagcc taagaaagat agaaaaataa atataactca agaagattta gataagcttt
      481 gtgaagtata tgattggtat ttagaagtaa aagattacaa tataaagcct aaaaaagtat
      541 ctaataaaaa agatgtacat atagatgatg aacacataga tgaattaaaa acaacaagaa
      601 tacgtataga taaagaaata tggaatgatt ttgaaagatt atgtagtaat tcgaattttg
      661 aaaaacatca aatacttaca caggcattga aagaatttat gaaaaagtat aaaaatctac
      721 tataaaatac tactatatga ggtgcttaat atgaataaaa tagtaaaaaa atcaatttct
      781 ctaatgatta tattaacaat atttattttt atgttgacag catgtgaaaa agatgaacag
      841 cctgattcag ttgatattca aacagaagat aaaaatgaga taaaaataga tgagggaaat
      901 gcaaaagtat tgaatatagg aaaatcagaa attataaata tagatgaagg tgataaagta
      961 gatacatcaa caaaggtaga aaataattca acatttaata tatccaatgt tgaattgata
     1021 tataatgaat atgatgagaa taaaaaaatt atatcttcgg atagtaaggc tttgttggat
     1081 atgactttaa tgcctgggaa agttgcttac atagaatgtg ggcataaaac ttttgtaaag
     1141 ggtgtagaag tttatgcata tgaatatgag gcagagggta agattgtgta tgttaattta
     1201 aaagaaaata ctattaatat aagaaataat aatattaagc tagaaaatag tagtcagtat
     1261 gaggtgctat ctacatcaga acttaaaaaa gtaaatgaat caaatgaggg aattacgtat
     1321 caagtaaaag taaaaaattc atcatcaaaa gatttaggaa atattattct taagacagca
     1381 gaagttaatg aaaatggaga atatttaaca gtaaatcgtg taccatcata taagatttta
     1441 aagccttcag aagagacaga tatagatata ttgtgttcaa caaaggcaaa aagtgttgaa
     1501 atagtgggat atacgtatga tgatataaaa gaaaaagcca atgtggatat tgacttaaaa
     1561 tctcataaag ttaagataga caaataaagg taggggatac ttatgaaggt tttaatagtt
     1621 gaagataata agatactttt agaaagtgta gtagaagaac ttagcaagca ttttgaaact
     1681 gaaaagtgtg aagatggaga agaagcttta tacttagtaa atcaaaatat atatgattta
     1741 gttattttag acttaatgct tcctaatata aatggatttg atatcctaaa gaaaatgaga
     1801 gttaataata tagatacacc tgtacttata ttgacagcaa aagaaacatt agatgacaaa
     1861 gtagaagcat ttactatagg agctaatgat tatttgacaa aaccttttta tatggaagag
     1921 ttagtagcta gggtatacgc tatattaagg actaatggaa agataaaaga aagaaacgga
     1981 ttagaattta aatctttata tttagataca ctagaaaaaa gagtgtatat tgaaaaagaa
     2041 gaaataaaac ttcaaaacaa acagtttaac ttgcttgagt attttgtatt aaataaaggt
     2101 tcaattttat taaaagagca gatttatgat agaatatggg gaattgattc agatgcaact
     2161 attgaaatag tggaagtata tgtaagtaat ctaagaaaaa agctaagtaa atatggatat
     2221 gataaatata ttaaaactaa acgtaaggta ggatatatat tcgatgataa ataagaatgt
     2281 atttaccagt actaaaaatc atttaataaa aatgtatatt attgtagtag gtagtttttt
     2341 aataatcttt tctatattta tatactcata ttttagggga cttacatata gtggaattga
     2401 tagtgagata aatgatgaac ttgaatatat agtctctcaa tttaaaagaa cttcattttt
     2461 gaacccaata agactgaaag atcctaaaga tatggtgtat gtatatgaag atggaaggat
     2521 aagttactat actcaaaatg aatattttga tgagttactt ccagatagaa gactagataa
     2581 aaaaaattcg ttttttaaat acacagaaaa tggatatact tttagagaat taaatgtaga
     2641 tgtaggtaga taccaaatac agataattag aaatatagat tctgagatga attctttaag
     2701 acagcttaca tctgttctta taattggaat attaatttct gtaataatta cttactttgt
     2761 ggctgtatat ctcactagaa aagctttaat tccaatagaa acagcatgga aaaatcaagc
     2821 taagtttata caagatgctt ctcatgaatt aagaacacca ataacaatag tttcatcaaa
     2881 attagaaagt atgttaaaat ccccagaaag tacagtaaat gatgaagttg aaacaatagc
     2941 tactgcaatg aaggaaacta gaagacttaa aaaaatgata actgatttat tgtctcttac
     3001 taaagaggac tctattgtta aagttaattt agaagaaata gacttagaaa aattattgga
     3061 agaaatatca gaagactata ttgacattgc tgagtttcag gaaaaaagat tcgtgtttaa
     3121 ttctaaactg aagaataaag ttataataac agataaaaat aaattaagac agcttatatt
     3181 aattttcatt gataatgcct ttaaatatac aaaattgggt gatgagatag cattagaatt
     3241 aaaagaagat atagaggatg aggtaacttt acttataagt gacactggta taggcataaa
     3301 aaaagaagag atacctctta tatttgatag attttttaga agtgaaaatg taagaaataa
     3361 agatttagaa ggttctggaa taggactttc aatagctcgc atgattagtt taaacttatc
     3421 tattgatata aatgtaactt ctgatgtaga tattggaact acatttgaat tgtcaatacc
     3481 aaaaaagtta aaataataaa aatataaaaa attgttttta gaaatgaaga atgggtatac
     3541 taattagtat aaaatataaa aagaccttat aaaagtaaat ttatatttat aaatttcata
     3601 gtataattat atagggataa taaagtttaa taggaggatt taaaaatggc aaaagttata
     3661 aatacatctg aatttagaag tagtgttgaa ggtagtaagg gagtagttgt agttgatttc
     3721 tttgcaactt ggtgtggacc ttgtaacatg ttgggacctg tatttgcaga attaggtgaa
     3781 gagatgaagg ataaagctag atttgtaaaa gttgatatag atgagagctt agaaatagct
     3841 caacaattca atgtatctac agtaccaact atgattatat ttaaagatgg aaaaccagta
     3901 gaaactttaa ttggattcat gccaaagaac aaaattgaaa tgcaagtaaa atcatattta
     3961 taaaaaatgg gtgagtatta tgagatatga tatagctata atcggcagtg gtccagctgg
     4021 attgtctgct gctataaatg caaaaataag aaataaaaca attatcatgt ttggtaatga
     4081 taatttaagt aataagcttg ttaaagctcc ttcaatagat aattatttag gtttttatga
     4141 tataagtggt gatgaattaa aagacaagtt taaatcacat atagatagta tggatatttc
     4201 aattgaaaat aaaagaataa ataatattta tgctatggga gaatacttta caataatgtc
     4261 tggaaatgat atgtatgaag ctactactgt aatccttgca actggggtag agtatactag
     4321 accaatcaag ggtgaagaag aatttctagg aagaggtgta ggctactgtg ctacatgtga
     4381 tgcaccactt tatagaaata aaaaggtagc agttatagga tataatgaag aatctaaaga
     4441 agaagcaaat tttttaagtg aactcacttc aaagacttat tttataccta tgtacaaaaa
     4501 agataatctt atgagaagtt cagataacct agatgattct attgaagtta tacatgacag
     4561 accagtacag atagatggag ataaacttgt aaacaaagta tcttttaaag aaaaccacat
     4621 tgaagttgat ggagtattcg ttataaaaga tagtacggct ccaagtgcat tggttcctgg
     4681 aatagagatt gatggcattc atattaaagt tgacaataat atgaagacta gtatagatgg
     4741 atgctttgct gctggagatt gtgttggaaa accctattct tatataaaag cagctggtca
     4801 agggcaaata gcagctttaa atgctgttta ctatttagat aaatta
//
LOCUS       DACTGW010000241         4846 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.244, whole genome shotgun sequence.
ACCESSION   DACTGW010000241 DACTGW010000000
VERSION     DACTGW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.244"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..418)
                     /locus_tag="I9573_003221"
     CDS             complement(<1..418)
                     /locus_tag="I9573_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAT4797814.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPI"
     gene            complement(531..1235)
                     /locus_tag="I9573_003222"
     CDS             complement(531..1235)
                     /locus_tag="I9573_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAT4797815.1"
                     /translation="MKKKILIPVIMSLFIISQCITSFAFTPENNKFKVKPLPYAYDAL
                     EPYIDKETMKLHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVR
                     NNAGGAYNHKFFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWA
                     WLVATKDGKLSIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVV
                     NWNGALENYKNLKSQD"
     gene            1408..3282
                     /gene="asnB"
                     /locus_tag="I9573_003223"
     CDS             1408..3282
                     /gene="asnB"
                     /locus_tag="I9573_003223"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAT4797816.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(3374..4810)
                     /locus_tag="I9573_003224"
     CDS             complement(3374..4810)
                     /locus_tag="I9573_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HAT4797817.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVHFTRY
                     IGVDDSIDKYIEKVKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIAQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
BASE COUNT         1868 a          585 c          700 g         1693 t
ORIGIN      
        1 taatgggata aaacaattca ataccttctt tagtaataag gtcaagacac aagtgagata
       61 gatatcctat tataaaccca ctggacaaac atgagaaaac tatattattg ttagtgaaga
      121 ttgtcagaga tttaaaaaaa ctataaataa gtaataaggc aattagactg tgagtaaacc
      181 ctctatgtct aaacctactt ccaaataatt tgtatattaa cataaatttt ttacttatgt
      241 aagaacctct catatctata tctggaaata aagaacctaa atatgcaaaa taaatataaa
      301 ttataattaa tactatttta tatgcaatat cgtatttaac taggtacaga ttgtttataa
      361 aggggagtat caaaagtgcg aatatataac ctcccctttc atgtgtttct tttatcatat
      421 aaaaatcctc ctgaatcgtc actataaaca ttatattggt aaatttagac tatcttttat
      481 tatattatat agcaaacata agtttttttc aaaaggattt taatatttaa ttaatcttga
      541 gattttaaat ttttgtaatt ttctaaagca ccattccaat ttactacatt aaaccagttg
      601 tcaatgtatt catttcttct attttgatat tttaaatagt aagcatgctc ccaaacatca
      661 agtcctatta taggagttag gtttttactt acagggctat cctgatttgg agtagtcata
      721 atagataatt tcccatcttt agtagctaca agccaagccc aaccagaacc aaagacatct
      781 aaagcagatt tttggaactc ttgcttaaat ttttcaaaag aaccaaagtc tctatcaata
      841 gcttctttta aagattcaga aggtatggtt ttttctggcg tcattatatc aaaaaagaat
      901 ttatgattat aagctccacc tgcattattt cttacagttg tagcaatatc tttaggtaaa
      961 gaatctaaat tttgcaataa ttcacataaa gaataattat aaagctcagg atatttttca
     1021 agagcagcat ttaatttatc aacataagct tgataatgct tatcatgatg cagtttcatt
     1081 gtttctttat ctatataagg ttcaagtgca tcatatgcat aaggtaatgg tttaacctta
     1141 aatttgttat tttcaggtgt aaaagcaaat gaagttatgc actgtgagat tataaataaa
     1201 gacataataa ctggtattaa tatttttttc ttcataaaaa tcacctccta atatagtatt
     1261 tctagaaata aaaaaaatat gaaatattaa cagataattt ataagaattt aatagcaatt
     1321 tcattttaaa ttgaataagt tttaaagcca aagacaaaac tatgaaatat agaataagac
     1381 taaaggacag aaaggaattt tgattatatg gacggcaata tttcaattta ttataaaggt
     1441 gatatatcta aaaaagaaga tattaaattt aaaataaaag aatctgtaaa taatacaaga
     1501 ctttctagta gaatagagta taaggatgag gaaaaaactt ttgattttga aagatttaat
     1561 gtaattgatg ctatttatgg aaataaacca tttgaaaaag atggaaaagt tattgttttt
     1621 aatggaaata tatataacta tcatgaaata aaagaagaat taattaaaaa aggacacaat
     1681 tttactacaa atgatgatat tgaagtctta ttggcatctt atatagaatt tggtaagaat
     1741 tgtgtacata agattaaagg aatgttcaac tttataatct atgatagaga aaatgaaagt
     1801 atatttggag caagagattt atttgggata aaaccacttt attatataaa taaggaaaat
     1861 gcaataattt tttcctcaga gtataagttt ttacttgaat atatgaaaaa tttaaatata
     1921 aatgaaagaa gcttacaaag ttatttttca tttcaatatg tacttcctga agatactatg
     1981 atacaaggaa taaggctgat accagcaggt cactatttta gggtagaaaa tggtatacta
     2041 tctttaaaga gatataataa acttgagttt agaagtagta caaaattttt ttatactaaa
     2101 aatcacttag gcaatagaga tgtgaatgag gatgatataa gaaatatagt agtagattca
     2161 atagtaactc atatggaaga ggagaaagaa attggaacat ttttgtctgg tggaatagat
     2221 tcatctatta taactactgt ggcttcacaa attaatccaa atataaaatc tttttctgca
     2281 ggctttagtg tcaaaggata tagtgaatta gaagtagcaa agaaaacagc tgataagtta
     2341 ggtatagaaa atatccagat taatataaca caagatgaat atataaaatc attaccgaat
     2401 gtaatttatt ctttggatga ccctatagca gacccttcag aagtaggtct ttatttctta
     2461 attaaagagg caggtaaaca tgttaaagta gcattgtctg gagaaggagc agatgaatta
     2521 tttggaggat ataatatata taaagagtat agtactatga aatcagttgt caattcacct
     2581 acttatatta aaagtatatt aggaagagtt tcagaactta tgccaaacat aaaaggaaga
     2641 aattatcttt atagagctac aacaccatta gaaaaaagat atataggaaa tgcaaaagtt
     2701 tttgaaaaca gcgaagtaaa aagattcttt tttaagtata aggaaaagaa tatatatgaa
     2761 tacctgttgt ctaatttgta tagagatgca caaaaaaata attacgatta tataagtaaa
     2821 atgcaacata tagatgtgaa tacatggctt caaggcgata tacttcaaaa aattagcaaa
     2881 ctttctacag ctgagcaggt tgaacttaga gtaccatttt tatataaaga tgtatttgat
     2941 gtagcaaaaa atttaagaat ggagcaaaaa ataaataaga ataatacaaa agttttactt
     3001 agagaggcgt ttagagaaat agtcccagaa tatgttgttc aaaggaaaaa attaggattt
     3061 ccaactccaa taagagtttg gttaaaagat tctctagggg atattgtaaa ggaaactatt
     3121 tataattcaa atgtagatga atttatagat aaaaaatatg caataaaact tttagatacg
     3181 catcttaaag gtcatagaga taattcgaga aaaatatgga ctatatttac attttgtcta
     3241 tggcatcaat tatttattga gcataaaaat gtcgagtatt aatacttttt tcaatattaa
     3301 tcagagggca aaagcatgca atgttttcac cctcttgatt attatggaat aggataagta
     3361 gaattttgat tgattagttt actatctcaa taaaattatt ggcaaatatt ttgacaagat
     3421 tactttcaac agctaaattt aagttttgtc catcatcata tccagcggaa atcaatccta
     3481 gtaagtttcc aaacatatct aaaagagctc ctcctgaact accactagag attggagctg
     3541 taaattgtat catttgaacc gtttcaaaat cacgaaaagc tgagataatt ccatctgaaa
     3601 ctgtattaaa aagacctaaa gggcttccaa ttgcaactat tttttgacct ctcacaggtt
     3661 ttttactgac tttaacaggt attggtttgc attttctatc aacctttatc acagctaaat
     3721 cataatctga atggtatttt ataatttggt aagatgtata aactttatca tcattttcaa
     3781 atctaactaa aaatgaatac ccatcattga ctacatgaag attcgtaagt atataacctt
     3841 cattatttat tactactcca gaaccactct taaacggttt attttcgtta ttgcacacaa
     3901 caatcataac aacagaagct gataactttg caaggtcttc ataagaaagt tctacttcct
     3961 catcttgtgc aattttttta gatgttagat ttgtgttatt aagtgtattt ttagagttag
     4021 gtttattcgt agtaggagaa gtcttattgg tattttcact gtaatttata tttgtaatat
     4081 gcctttcaaa aggtatttct aatatatttg gagttttcct agaaagttca aatagagtag
     4141 aaaattttga acattcagga tataaatcaa gaatatcttt atttggattt atatataaaa
     4201 tatcacaccc aagttgtgat aaaaaatata agaaaaaaag ttcttgcctt ttaatatttc
     4261 caaagtatac tatctttggt gaaatagtag tcttcacctt gttataaaat aatttaggta
     4321 gaaaatagtc catccaacac aaaagtttaa ctgcaaagtt tttgatgata gaggatgtac
     4381 aatttggcat acttgtggaa aataaatcta aagtattttt aaatgcaatc tttttagtcc
     4441 aatttaaagt gtcattttct attttacatg gaaagtcgat attatataaa tcccaactgt
     4501 taaacttaga actatcataa atattaaaaa ttttaagcat cttatctgtt tgttctttgt
     4561 caaaatttat ttttagattg tttaatctaa tatagggaat tgtaggatta ttaaaaaaat
     4621 aagtatctat atttttaact ttttctatgt atttatcaat tgaatcatca acacctatat
     4681 atcttgtaaa gtggacttct ttaatatatg aagaactctt atttttatag gtagtatcag
     4741 atataaattc aattagaggt tcagttgact tttttattga aatctttaat ggtttaatag
     4801 tacaaatcat attttttctc ctataataag atgatataat ctatta
//
LOCUS       DACTGW010000242         4839 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.245, whole genome shotgun sequence.
ACCESSION   DACTGW010000242 DACTGW010000000
VERSION     DACTGW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.245"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(416..883)
                     /locus_tag="I9573_003225"
     CDS             complement(416..883)
                     /locus_tag="I9573_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HAT4797818.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKSEKWVDVCKNW"
     gene            complement(962..1504)
                     /locus_tag="I9573_003226"
     CDS             complement(962..1504)
                     /locus_tag="I9573_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797819.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITESTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(1556..2242)
                     /locus_tag="I9573_003227"
     CDS             complement(1556..2242)
                     /locus_tag="I9573_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAT4797820.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(2230..3102)
                     /locus_tag="I9573_003228"
     CDS             complement(2230..3102)
                     /locus_tag="I9573_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797821.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDREKFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVRGCSI"
     gene            3237..4037
                     /locus_tag="I9573_003229"
     CDS             3237..4037
                     /locus_tag="I9573_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HAT4797822.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            4062..4631
                     /gene="yfcE"
                     /locus_tag="I9573_003230"
     CDS             4062..4631
                     /gene="yfcE"
                     /locus_tag="I9573_003230"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAT4797823.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT         1738 a          736 c          522 g         1843 t
ORIGIN      
        1 atatttatat tagggtatat taaacattaa atattgtctg tacatctgtt ttttagccat
       61 gtacagaaga tttttatcgt ataaaaatta ttttaataga gctaaataat cgtattttta
      121 tctgactatt ttttattttt aaatatctta catctataat agtaacactc aaaggataat
      181 ttttcaataa tttctaatta tttttttaac aatatttatt acattttttc cacttttttg
      241 tcattttttt attgtttcga aaaaaaatca gaatttttta actttatttt ggattattag
      301 tttaaattag attttcctct tagatttata aatatttaca caaaaaacta cctctgtata
      361 gattgtatac agaggtagtt ttttactaat tcctactata gtttactata tttttttacc
      421 aattcttaca aacatctacc catttttcac tcttagaaaa cttctccatt tcttcacatg
      481 taagttctat tgcagagtta ctactgccac atgcaggaaa tactgtatca aatctcttca
      541 ttgaatcatc taaatagact tttatagaat tttttaaacc aaatggacag actccaccaa
      601 tagcatgtcc agtaaactcc aagacttctt ctggtgtcaa cattttagcc ttacaattaa
      661 attcagcctt atacttctta ttatcaatct tagcatcacc agctgtcaca attaaaattg
      721 catcatctcc tattttaaat gagagtgtct tagctattct tgctggaata accccagctg
      781 cttcagctgc aagttccaca gtagcactag attgttcaaa ttctaatata ctgtcttcct
      841 tgccaaattg tttaaaatac tctcttacat tacttattga cattaaatac ccctcctagt
      901 ctgaattttt ataaatttca tattattctt aattatatct ataattgatt tatttttcaa
      961 attattccat aatattagca ttccatgtat cattattttt tccaactact agataaagtc
     1021 cttttgccat tggaatattg acaatatatt ctcctttata ttctacactc ataccagatt
     1081 cattaaagta cctataatca aaatttagtc cttttggaat tacaaaatca acataacctt
     1141 gattatttgg ttttattcca tatttcagag tactttcagt tatagaattt ttttcattaa
     1201 ataaatctac atttacatat gcagaacact tattcattcc ctttttattt tccacacaat
     1261 tttcaaactt cattttagca ctgtttaatt tataactata tttactatta ttgcttattt
     1321 taaatcttac aatagtaccg atatctctac ttctcctgct atcaaaatca aaaacaattt
     1381 cctctaaatg atttatttca taatctttga cacttaccat atctctatat actaatttat
     1441 ttatagctat gaatagtgta aaaatcccca aaataaaaaa tgaaactttt ataaacttct
     1501 tcatttttag tctccttata ttttgtgcta ttgtgctata atattaatta aaattttaat
     1561 aattaaactt attctttttt gaaatcttaa tagaaaaata cattgatatt ataaaaatta
     1621 ttattgttaa aaatgtcata ctatataaat ttatatactt agtattagct atatcagtta
     1681 cataatttga aagatatact cctccaactt tactataatt aaaatgcttt acttgatata
     1741 caatcatact aatcatcttc aaaagacttc catatacaaa gtaaattaaa atacttacta
     1801 taaaaccaca aactttcatt tttccatatc caaacttaaa aattaaaggc agtaaaatcg
     1861 tcaaaattgc taaaaatata tttacagaaa aaaatacatc atttaaaacc atatttctaa
     1921 agaacactag tgatgtcaat ttacttagta aggacacaat tattgttaca gaaattatta
     1981 gtcctacacc taataaatat tttgagtaaa ctatatcttc tatactaact ggcatactcc
     2041 ttataaaaat ccttgaatca ttttcataat cacactcaaa tgtatttata aatatcaaat
     2101 aactaacaac aatacttact gtataatatg aaattgaatt aaggaaaaaa taaaacacaa
     2161 aaaatagcaa tatgtacttt aaattacgga taccatctga actaattcct attattaaat
     2221 cttttcttat taaattgaac atcctctcac ctcatttaca taataataca ttagttcttc
     2281 tatagtagga tttttgacct tatgcttatc aatttctatc aataggcttt ttactttcaa
     2341 gctatctcca actttaacaa gtgcctcatt ataatattta tactctttaa caccaactat
     2401 actttcttta attgcttcta actctgcttt atttgcccta attatcttat attcttgttc
     2461 tattaattct ttcttaacag taaaaatctg atttcctcta tttataaaaa caagataatc
     2521 agcaatttta attaaattat cagtattatg agtagaaatt attacactac tacttccatt
     2581 tttcacaaaa tcttgtaagt actgcattat ttcagttctt acaatcgggt ctataccagc
     2641 agttggttca tccaaaatca ataacttagc attatgacta agtgcgcttg ataacataag
     2701 cttctcagat tgacctttag ataaattttc aatatgcata ttttcttcta tgtcaaactt
     2761 attaaggtat tcatcaaatt tttctctatc aaaattctta taaaataaag aaactatttt
     2821 tctaaattcc ttaaccttta aatgtggata aaattgtaaa gaatcatata caaaaccaat
     2881 attgttttta aagcttattg gagaattttc aatagtttca ttacaaagtt caatttcacc
     2941 ttcatctgca tcaataatcc ccattataat ttttattaaa gtcgtttttc ccgaaccatt
     3001 tggtcctata atcccaacaa tatccccttc ctttaactct aagtttatat tatttaactt
     3061 gaattttcct aatttttttg tgatattcct tatgctaaac acatcttcac ctcttatgca
     3121 tttttttata cccacctata catattttaa cacattcact acaaaggtca tttataaatt
     3181 tataaattaa gaattataaa actatgttat aatacaaagt atgaagaggt gaatatatga
     3241 aaagaattat aactataaat acaaaccaat taaataagtc ccttgagact aaagaactct
     3301 taactagaaa acttatcaat gctggctttg aagtgtattc agatatttat ccagacacag
     3361 agcttattat atcaattggt ggtgatggct cttttttaag aacagttaga gatttcgatt
     3421 ttcctgagat tcccataatg ggaataaata caggtcatct ggggtttttt cctgatattt
     3481 taccagataa gattgatagc tttattgaag cttatacaaa aaaagattat attattcaag
     3541 aaatgtcact tcttaatgcg gaagtatata caactactag tggttctaat atgcttgctg
     3601 taaatgaggt tgttataaga ggagacaagt ctaggactat acacctaaat ttaagccttg
     3661 ataataaaca tatacaaaat tttagtggag atggtatgat tatttctact tccacaggtt
     3721 caacagccta taactattct gctggaggta gtattgttga tataaatctt gaacttatgc
     3781 agattacacc tttacatccg ataaatacca atgcatatag gtgctttaca tcaagtataa
     3841 tatgttcaaa tgaatctgtt ataaagattg caccagaata tagatttgaa gattctgtat
     3901 tgattgtcgt tgatggagtt gagcacagat ttagacaaat cgaaaatata aaagttagta
     3961 tatctgatgc aaaaataaaa ttacttagaa tgtcaaacta tgagttttgg catagagtat
     4021 ctgaaaaatt tttatagact gtacttagga ggatttttaa tatgaaaata ggaataatga
     4081 gtgatactca tggtagctta ctttactttg aaaaagcgtt aaatgtatta tctgattgtg
     4141 atgttttatt acatggtgga gatgtcttat atcatggacc tagaaatgat attccagaag
     4201 gatataatcc taaaaaattt attgagacat taaataaatt agaaaacatt gtgattgtaa
     4261 aagggaactg tgatgcagat gttgaccaga tggttataga acatcctatt cagagtcctt
     4321 atgtcatgag ccaatttggt gaaattagaa ttatattgaa tcatggttat atagaatcag
     4381 aagaagaaat tattgacaaa gcaaagaaaa tgggtggtga catattagtt ttaggacata
     4441 ctcatgtaaa aaaactatac atggatgata atttaatagt aataaatcca ggaagtactt
     4501 ctatcccaaa agatggaagc cattctgtcg caattataga tatcataaaa actgatgatg
     4561 aggatgagct tgaacttgat ataaacttaa ttgacatcaa tactggaaat ataataaata
     4621 tcaatgatta gtaaattttt aataaaaata aaataagctt taatttatta aactcataat
     4681 aaaaacaagt aaaccatttt atttctatct caattagaag tttaagtaca ataccttaaa
     4741 cttctaatta aatagaatct ggtttacttg ttttattata tgctatattg aaatataact
     4801 tagtttactt gtttctatta tatattatat tggagtata
//
LOCUS       DACTGW010000243         4808 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.246, whole genome shotgun sequence.
ACCESSION   DACTGW010000243 DACTGW010000000
VERSION     DACTGW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.246"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            44..442
                     /locus_tag="I9573_003231"
     CDS             44..442
                     /locus_tag="I9573_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAT4797824.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSEKYKLKARNVK
                     INLNIKGYKFLPYVKNLKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            430..1368
                     /locus_tag="I9573_003232"
     CDS             430..1368
                     /locus_tag="I9573_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAT4797825.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIRH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKTIILGVCGKLILVPSIFLPISIFLGFRNVELTSLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1752..1976)
                     /gene="secG"
                     /locus_tag="I9573_003233"
     CDS             complement(1752..1976)
                     /gene="secG"
                     /locus_tag="I9573_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAT4797826.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2094..2600)
                     /locus_tag="I9573_003234"
     CDS             complement(2094..2600)
                     /locus_tag="I9573_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4797827.1"
                     /translation="MNLEIIRATSENAIEMGFIHSCSWRKAYKGIIPDKIIDEFTSEK
                     RAEVFQSVIPKAEEEYYLFKVNGIPAGFASLNRSQEDNAPKCVGEIYSIYFHPDFWGT
                     SVTKKGLQFCIDRLSNLGYSYITIWVLKDNTRAINFYKKNGFTCDDFEKEIHIGKKLL
                     EVRYSKKI"
     gene            2685..3113
                     /locus_tag="I9573_003235"
     CDS             2685..3113
                     /locus_tag="I9573_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAT4797828.1"
                     /translation="MNPVKELNNLWHIVSMQMKTEMCSGKYPTIHGISLVELSILEIV
                     EQYPNSMMKEISEILELPKSTLTSAVKRLELKKFIKKNPCIKDKRRYNLVLTELGLKA
                     QQEHRDIEIIVFRNLLHQLDKDEIETFIRIFSKAVNKSKK"
     gene            complement(3233..4525)
                     /gene="eno"
                     /locus_tag="I9573_003236"
     CDS             complement(3233..4525)
                     /gene="eno"
                     /locus_tag="I9573_003236"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAT4797829.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTERLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1657 a          765 c          575 g         1811 t
ORIGIN      
        1 tatataaatt taactaattt atataaaatc ttaaggagtt gccatgataa aaaaaataga
       61 gcatgtggtc attactaata ataatataga aaattatatc aatttttata aaaaatttgg
      121 tttttcatct aaagaaatga gcgaaaagta taaacttaag gctagaaatg ttaagataaa
      181 tcttaacata aaaggttata aatttttacc ttatgttaaa aatttaaaag caagtagaga
      241 taacttatgt tttaaaataa acacaaatat tgatgagcca aaaaaatact tagaacaaaa
      301 tgagattcaa attaagcgag gaattgtaga atgtacaggt gtctttggta atatgaaatc
      361 tatttatttg aaaaaaacta atggaaattt aattgaatta tcaaatcata gaatcacgga
      421 gagtaaacct tggaaaattt aatcctatca ataaatgttg ttcttccttt gtttctcaca
      481 atgtcacttg gatatgttct aaaaaaatta ggtctatttg atactcatac tttaaataaa
      541 atgaataatg tctgcttcaa gtctttcttg ccattattat tattttttaa tatatacaat
      601 acagatttag atggttctct taattttaag cttatggctg tagctgttat aattattttt
      661 tcaatcttct taatcttagt ctttattatt cctaaaattg aaaaagataa taagacaagg
      721 ggtgcattaa tacaaggtgt atttcgtagc aatttcgtta tatttggtat tcctgtgaca
      781 atttcactat ttggcgataa gagtattgga gtcacatctt tattaattgc agttattgta
      841 cctatgttta atgtattatc tgttgtaatt cttgaaattt ttagaggtgg caaagtaaat
      901 ataagacata tgattaaaag cgtcttaaca aatccactca taatcgcctc ttttatagga
      961 ttgacaatgc taattttaaa tattaatata cctacatttt tacacaaaac tattggtgat
     1021 atttctaaaa tagctactcc tttatcactt ataattctag gcggatcttt tagtttttct
     1081 gctataaagg ggcatataaa aaccattata ttgggcgtat gtggaaaact tatacttgta
     1141 ccttctatat ttttaccaat tagcattttt cttggattta gaaatgtaga attaacttca
     1201 cttatgataa tgttatctgc tccaactgca gtttcttcat ttacaatggc acaacagatg
     1261 gattctgaca gtgaattagc tggacaaatt gtagtattta cttctggatt ttctataatt
     1321 acagtatttt taataatatt tattttaaag cagaataatc ttatttaatg atatttgata
     1381 acacaaattg gtattatgag aaacatatat aaaattgatt taattgcgta aaaacatatt
     1441 attatactat ttttactata taatataact ttgtgtatat tatctcatta aacatggtga
     1501 aagtagtgtt gctatatatt ttttagtttg aaaaaaatat ataaatataa acaatatata
     1561 aatacaaata ataaatgcca acaaatagtc aatctataaa ataaaaactg gactaatcat
     1621 ataaaatcat tagtccagct tttattttac gtacttttta tgttgatgta cttttattaa
     1681 aaactaaatt taatttttaa atatctagca aatgcactaa ttaaataatg gccagttatt
     1741 caacaatatc actacttctt aactataagt aatgccaatg catttataaa aaataatact
     1801 gctgttatct ttgtaactct agcaagaaat gcttgttttc cttttggttt aaagtaagct
     1861 tgacttcctt ctccaccaaa ttctgaaggt aaagcatttt ttgaatcttg aggcattatg
     1921 ctaattacta gtaaaatccc tagcactact tgcacaccca ttaaaatgtt gctcattatt
     1981 gcccctccta tctatttatt acttaaattt cattactctt tagtgtatca caataatata
     2041 gtcatttcaa gactattgaa gtataaataa caaacaaaaa taatatgcaa ttactatatc
     2101 ttttttgaat aacgtacttc taataatttt tttccaatat gtatctcttt ctcaaaatca
     2161 tcacaagtaa aaccattttt tttatagaaa tttatagctc tagtattgtc ttttagaacc
     2221 catatcgtta tatatgaata tcctaaattg cttaatctat caatgcaaaa ttgtaatcct
     2281 ttttttgtca cagatgttcc ccaaaaatcg ggatggaaat atatagaata tatttctcca
     2341 acacattttg gagcattatc ctcttgactt ctatttaatg aagcaaaacc agcaggtata
     2401 ccattcactt taaaaagata atattcctct tcagcttttg gaataacact ttgaaaaact
     2461 tctgctcttt tctcagaagt gaattcatct attatcttat ctggaataat tcctttatac
     2521 gcttttctcc acgaacaaga atgtataaaa cccatttcta tagcattttc acttgtagct
     2581 cgtataatct ctaaattcat aaaaacacca tcctattgac aaattaatac gattatcgta
     2641 ctatattagt gcgataattg tatcatatat ggaggtaaaa gtcaatgaat cctgtaaaag
     2701 agctaaacaa cctgtggcat attgttagta tgcagatgaa aacagaaatg tgtagtggta
     2761 agtatcctac tattcatggt atttctttag tagaattaag tattttagaa atagttgaac
     2821 agtatcccaa tagtatgatg aaagaaatta gtgaaatttt agaattgcca aaaagcacat
     2881 taacaagtgc tgtaaaacgt cttgaattaa aaaaatttat taaaaagaat ccctgtatta
     2941 aagacaaaag gaggtataac ttagtcttaa ctgaattagg tttaaaggct caacaagaac
     3001 atagagacat agaaataatt gtatttagaa atctcttaca tcaattagat aaagatgaaa
     3061 tagaaacttt tattcgtatt ttttctaaag ctgtgaataa aagtaagaaa tgaaatataa
     3121 aaaaaggata agtgaattga aaaatcaact atcacttatc ccttataaga taaattttta
     3181 tcttttatgt tatcttttta atctttaatg ccatattttt aatcttcaat aactattttt
     3241 ttaaattata gaaagatttt aatccacagt atctagcttg ttctccaacc atttcttcta
     3301 ttcttaataa ttggttgtac ttagcaactc tatcagttct tgatggagca ccagttttta
     3361 tttgaccagc atttactgca acagctaaat ctgctatagt agaatcttca gtttctcctg
     3421 atctgtgaga tataacagca gtatatcctg ctctcttagc catttctata gcatctaaag
     3481 tttcagttat tgtacctatt tgattaactt taactaatat agagttagct actccatttt
     3541 caattccttt ttctaatctc tctgtgttag taacaaataa gtcgtcacca actaattgta
     3601 atttatttcc taatctttca gttaataact tccatccgtc ccaatcttct tcgtctaaac
     3661 catcttctat agttattatt gggaagttgt ttgaccaatc ttcatataaa gcaaccattt
     3721 cagcagcagt taactctttt ccttctcctg ctaaaacgta ttttttagtt tctttgttgt
     3781 acatttctgt agcagcaacg tcaagtccta acataacatc ttcacctggc ttatatccag
     3841 cttttgttat agcttcaact attaattcta aagcttctct gtttgaacct aagtttggag
     3901 caaatccacc ttcgtcacca acaccacaag ctaatccttt ttctcctaaa actttcttta
     3961 atgaatggaa tacttctgct cccattctta aaccttcttt aaagcaacaa gctcctactg
     4021 gtaatatcat gaactcttga acatcaacgt tattatcagc atgctctcca ccgtttaata
     4081 tgttcatcat tggaacaggt aattgtttag catttactcc acctaaatat tggaataatg
     4141 gtaaacctat ttcatcagca gctgctctag ctactgccat tgaaacacct aatatagcat
     4201 tagctcctaa tttacctttg tttggagttc catctaattc tatcatgatt gcatctatag
     4261 ctggttggtc aaatgcatcc attccttcta tttcaggtgc tattatttca tttacgttag
     4321 caacagctgt ttctacacct tttcctaagt atcttccctt atctccatct cttaattcaa
     4381 cagcttcaaa agctcctgta gatgctcctg atggaactat tgctcttccc attgctccat
     4441 cttctaatac tacttcaact tctacagttg gatttcctct tgagtctaat acttctctag
     4501 catatactaa ttcaataact gacatttgta tatctccctt cgattttcta ttagttaaat
     4561 tttcatttac tgtaaacatc cgtctactat tttaatctac tttctatatt atagtacata
     4621 aagttgcttt tgaaaattag gtacaaattt atttgatggg taaatttgta aaagttccta
     4681 tttttatttt attttttata atattttaat ttatataata taaaaaaact tgtctaaaat
     4741 ccaaaaccaa tcttaaatac acaaatatta taactagatt taaaacatta aacataaaat
     4801 gtacaact
//
LOCUS       DACTGW010000244         4763 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.247, whole genome shotgun sequence.
ACCESSION   DACTGW010000244 DACTGW010000000
VERSION     DACTGW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.247"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            328..1233
                     /locus_tag="I9573_003237"
     CDS             328..1233
                     /locus_tag="I9573_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797830.1"
                     /translation="MESKEKSILRATHFGIIKIGEKDLNCAVLENGSRIINKSTVFKA
                     FGRTKRGRKKDEIRVPSKPNLPSFIDAKNLSDYISDELEDMLINTIKYKTKNGRIVEG
                     YPAEIIPLICDVYLQARLDNKLAVQQEGLAIASEILVRSLSKIGIVALVDEATGYQYD
                     RDRDELQKILSMYISKEFLPWTKRFPDEFYRQMFRLKNWTYPRPSSKRPGIVGTYTNK
                     YVYDLLPPGVKEELQRVNPTVKPGQRKHKHHQFLTEDIGNDHLKNHLLKVITLMQASK
                     DWKDFNVLFNRAFNLPVQLEIEYDE"
     gene            complement(1907..2256)
                     /gene="ssrA"
                     /locus_tag="I9573_003238"
     tmRNA           complement(1907..2256)
                     /gene="ssrA"
                     /locus_tag="I9573_003238"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2414..2869)
                     /gene="smpB"
                     /locus_tag="I9573_003239"
     CDS             complement(2414..2869)
                     /gene="smpB"
                     /locus_tag="I9573_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAT4797831.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            3475..4302
                     /locus_tag="I9573_003240"
     CDS             3475..4302
                     /locus_tag="I9573_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HAT4797832.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT         1743 a          659 c          625 g         1736 t
ORIGIN      
        1 tacttataat gatttatttc attgcaatat ttgcaatagt aagttttcaa ttacaactaa
       61 taccattatg cataaaacta aactagacta cagaaaatgg ctacttgcta ttaatttatt
      121 tatatttgat gaaaatatta gctatagaaa attatcaaaa aatatcgttg ttaataaaaa
      181 tactgcgtac aagatgataa aaaaactcaa ttatctatat acatatttta aaattgatat
      241 tattagtctt ataaatacta atttatctaa tgaagagata ctaagtaata tcttacttat
      301 taaaataaaa taaaggatgg tgttaaattg gaatcaaaag aaaaaagtat attgagagca
      361 acacattttg gtattataaa aataggtgaa aaagatttaa attgtgctgt actagaaaat
      421 ggttcaagaa tcataaataa aagtacagta ttcaaagctt ttggaagaac aaagcgtggt
      481 agaaaaaaag atgaaattcg tgtaccttct aagcctaatc taccaagttt tattgatgca
      541 aagaatctat ctgattatat ttcagatgaa ttagaagata tgcttataaa tacaataaaa
      601 tataagacta aaaacggaag aattgtggaa ggttatccag ctgaaataat acctcttata
      661 tgtgatgtat atttacaagc aaggcttgat aataaattag ctgttcagca agaaggacta
      721 gctatagcaa gcgaaatttt agtaagaagt ttatccaaaa tagggattgt agcattagtt
      781 gatgaagcta caggatatca atatgataga gatcgtgatg agctgcaaaa aatattgtct
      841 atgtatattt ctaaagagtt tttaccttgg acaaaaagat ttcctgatga attttataga
      901 caaatgttca gattaaaaaa ctggacatac cctagaccaa gttctaagag acctggaata
      961 gttggaacat acacaaataa atatgtttat gatttattac ctcctggagt taaagaagaa
     1021 cttcaaagag ttaaccccac agtaaaacct gggcaaagaa aacataaaca tcatcaattt
     1081 ttaacagaag atataggtaa tgatcattta aaaaatcatt tattaaaggt tataacctta
     1141 atgcaagcat caaaagactg gaaagatttc aatgtgcttt ttaatagagc tttcaatcta
     1201 cctgtacagc tcgaaatcga atatgatgaa taatagacta tagcctttaa caacgcttta
     1261 aatactttta atttatatta taaaaataga attaacttat atcataaaag tacttattag
     1321 tataggtact tttatgatat attatttaaa ttttatatat aatatattgt ctatatacaa
     1381 tagtcttgtt agtattaaac ttaataattc tatgtgttta ttatcttaac atttttcaat
     1441 tcctttttta catcttattt ttttaaatac aattaaaaaa tttaaattag catcccacat
     1501 agataataaa tttgttctat acttatacat aatcttatag gattctgtat atcattataa
     1561 ctagtaatta ctctaataga ttaagtagca cttatatgct ttaaacttct ttatcctttt
     1621 aatcataagt ttaaacaagt tatatatttc ctagattgaa ataaaaagca taatataaaa
     1681 acaattcatc agtataaaat atttattgta ttatcatcaa attcactata tctctaaaag
     1741 tattctttat atgttccatg gattaatcaa atgcacccca atatacccca aaaaaatctc
     1801 tttttctgtg gttggggcac ggttggtgga tttcatctcc aaaacaaaaa acactagtca
     1861 ataaagacta gtatttccaa gtatttcatc attctttttt aaaaattggt ggagacgagg
     1921 ggagtcgaac ccctgtccgc aaatcttttc cttgctgcat ctacatgctt agccaacttc
     1981 actaatatct cacctcacat agcgccaagt ggcaggctct atgcttagcc agtcccaatt
     2041 aatctacttc aggtcgagac acccctaaag tagttccctg cataatgacg tccagtctta
     2101 gtcggcagga ggactaagag ggacgagcag caactaggct gctatcgcga aattatcgtc
     2161 tgcgtttatt ttaaagttcc ccagttttta cgtgacccag agtaacacga catgcagcct
     2221 caagttccaa acccacgtcg aaacctttac gcccccttga gtagtttata gtccagtaga
     2281 cttttcgcaa actttatcac catttttatt aaatttttaa tgtatatata atataatact
     2341 tttaagaata aaaatcaatg ttatgttcta gataataaag aattttattc taaaaaatac
     2401 agttttatct agtttaatac ttacctgtca tttctctttc tatacgtctt tgtgcatctt
     2461 tcttagccat atcttgacgt ttatcatata gttttttacc ttttgctaag gcaatctcta
     2521 ctttaacttt accgtttttt agatacaaac taagtggtat aagtgaatac ccctttatag
     2581 tagttgctcc aataagtttt cttatttcat gcttatgaag taatagtttt ctaactctta
     2641 aagaatcaac attgaatata tttccttgct catatggact tatatgaact tgttttaaaa
     2701 atacttcact attatctaca gatgcaaatc catcacttag gttaactttg ccttgtctta
     2761 ttgattttac ttctgttcct tttaattcaa ttccacattc ataagtttct tctataaaat
     2821 attcatgtct tgctcttttg tttgttgcta atattttttt accagccata taaatctcac
     2881 cttctttgtt agaaatttat tccataaatg ttagaaattt actaaatatt taatgttgcc
     2941 aaattatttg actaacttgt atgataacat atttaggcat tttagtcaat atttcactga
     3001 tatattaagt tagttaggct atatataatt aagtaatata aagatatgaa aatagctaaa
     3061 aaactacttc gaatatactt aatactaaac taactttaac cagtaacact gatttctaaa
     3121 tttcaaaaca aaaatattac atattataaa ttttctatct tctatcttct atcttctatc
     3181 ttctatcttc tatcttctat cttctatctt ctatcttcta tcttctatct tctaaataat
     3241 tttaacatgc ccataaaata taatacccac aaatgataaa taatactcaa aatatctatt
     3301 gaaataacta tacatcctta aataacttaa cttaaatcta aacatttcaa aaacttctta
     3361 aaaatatgta aaaatttgat gataaagtag cgattttttc aaaaaaaatg taatatagag
     3421 ttatatctta taatatttta gagataaatt ttatcatagt tgggagaaat ttgtatggat
     3481 ttaagcaaaa aagtaaaaat tttagaaaat gcatcagatg gcaaaagcga atttgaaata
     3541 ttagaatttg aaaaacttga gggagcttct agcccattaa tggcaatggg attatatttt
     3601 gctagagaag ctggtttgaa agctagacaa attaagataa aattaaataa ttcatcaata
     3661 aaaacagaag ctggtgcatt atactattat aaaggaaata tatcttctga aactaagatt
     3721 ggtggtgttg gaggtctttt taaaaaagga atatcaggag cacttactaa tgaatcaatg
     3781 atgaaacctg tatacaaagg tactggagaa gtttatttgg aaccttcttt tagacattat
     3841 ttatttatga gtcttgataa tgattctata attgttgata aaggattatt ctattgttgc
     3901 tctgatacaa tagaagtaaa agcatttaca caaaagaact tctcatctgc tttacttggt
     3961 ggtgaaggct tcttccaaat agaattaagt ggtactggtg taatcgttct ggaatgtgtt
     4021 gtacctcaaa gtgaaattgt agagtatgaa ttgaaaaatg gagaagaatt aaaggtagat
     4081 ggtaactttg caatagcaag aacatctggg gttaattttt ctgttacaaa atcagataaa
     4141 tctttaattg gttctgcttt aaatggtgaa ggtttcttaa atacatttac tggtaatggt
     4201 acagtttggc tagctccaac tcaagtaatg tatgaaaaac taagttttgg agcaatgcct
     4261 actaatgata atatgaataa taaagtttct aagcaaaaat agttgtttta taaatattta
     4321 ttaaatcatt attgatttta taaagtcact ataaatttta ttagattatt attataaaat
     4381 cagagtgtgg aaatgtttat gtgtattctt taaataaaaa tttccacatt ctgtaaggtt
     4441 attatacttg atatataaaa ttcaatttag gaacatagtc taacatttat aaacatatta
     4501 aggtaatgat ttctcttggt aattattctc atatcttatt ctttgattat atttatctta
     4561 ttcctagccc ttatgtcata ttatatttct tatcttatta tttataatta gcattaactt
     4621 atctaaaatc ttgtattttt ttaatagtaa agtttacatc tatattaaaa agtttacatc
     4681 tatattaatt tcataaaaca atcaaggtag tcaagagatt caactttatt tgattttaaa
     4741 aatttaagtg aatttgttct tat
//
LOCUS       DACTGW010000245         4735 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.248, whole genome shotgun sequence.
ACCESSION   DACTGW010000245 DACTGW010000000
VERSION     DACTGW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.248"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            759..1598
                     /locus_tag="I9573_003241"
     CDS             759..1598
                     /locus_tag="I9573_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797833.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            1588..2031
                     /locus_tag="I9573_003242"
     CDS             1588..2031
                     /locus_tag="I9573_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797834.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            2042..2533
                     /locus_tag="I9573_003243"
     CDS             2042..2533
                     /locus_tag="I9573_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797835.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(2735..3463)
                     /locus_tag="I9573_003244"
     CDS             complement(2735..3463)
                     /locus_tag="I9573_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HAT4797836.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            3588..4676
                     /locus_tag="I9573_003245"
     CDS             3588..4676
                     /locus_tag="I9573_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797837.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLIIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKELMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
BASE COUNT         1891 a          421 c          732 g         1691 t
ORIGIN      
        1 tcttaagtaa gtatgttact taatattata tgttaaatat gatttaaaaa ggttataaga
       61 atatttttgt attaaatata atcaaataaa tatatttatt tgtcataaat taaatgtcca
      121 ctaaaataga aaaaataaga agaaataggt aataaatgat agcttatagc aaaataaaga
      181 tgtaagtata acaatagatg agattggaat taagtcacta tacctaacag aacattctta
      241 ggcgaatata gaagacttaa aaaagaaaag agaagtagac aagaatttat taatgattac
      301 atataaaata aatgaattat aattttatta atgatataat aataattaac aaaaaataaa
      361 agaggaacga gttaatgttt gatggcatac actcattttc taaccaataa aacaatagat
      421 tcaattaagg ttactaaagt ctaatcaaca cataaacata taggataaat acatcttttt
      481 aatatattaa ttatgttaga cttacaaaaa atgtaaatac atagcttgtt aaatcttgat
      541 tgaaccttaa gtaataaggg ataaaaaatg acttgttcaa aggcaaagaa tatactatta
      601 aatgatataa gtaacttaga gataatatgt agtaagtcaa aagaactata tagcatcagt
      661 aagtaaaaag cgtaaaaact tacataatgc actataaaat aaatgagtta gaaaataaat
      721 ataatttata taataaagta gtacaaaaag gagtatttat gtttggatat gttaaaataa
      781 acaaaatgga tttaacattt agagaatatg attattacaa agcttactat tgtggactat
      841 gtaaatactt aaaaagaaat catggagaaa tctctaggtt ttctttgaat tatgatataa
      901 catttttaat tgttttatta acagcagtat ataacccaga gtctataagt acagaggaag
      961 tgtgtatagt aaatccattt aaaaagaaaa aggtaattac aaatgatatt acagaatatg
     1021 cagcaagtat gaatattctt ttaacgtatt ataaattgga agataattta atggatgata
     1081 aacgcataaa agataaattg gcatattata tatataaaaa caaattaaaa ttagcatatg
     1141 aaaagtatcc tgaaaaagca gagtatataa aacaacaact aaatgaatta aataagttag
     1201 aaaaagataa aaatataaat atagatgaag tatctagtat atttggaaat ataatgggtg
     1261 aagtatttgt ctataaaaaa gatgaaaatg agagaaactt aagaatgata ggttttaata
     1321 taggtaagta tatatatcta ctagatgcct atgaagattt agatgaagat tttaagaaag
     1381 gaagatataa tccatttata gagtatattg ataaaaatga tgagttaaaa gaaaaagtaa
     1441 aaaaaataat agtaacatca ttaggatttt tagctagggg aattgataat ctaaatctaa
     1501 ataaaaatgt tggaataata gaaaatataa tttattctgg tgtttattta agatatatta
     1561 atatattaga aagtagaggt ggaaagaatg tacagtaata cttataataa tgaaaatttt
     1621 atgagagcaa gagcattcat agaaaatagt gaatttaaaa aagcatatga ttttttaaaa
     1681 actttgactg acaaatgtgc agagtggtat tatttaactg gcttttctgc aatgaacata
     1741 ggatattatg aagaaggtga agacttctta aaaagagcaa aatttatgga gcctgaaaat
     1801 agtgaatata gtgatgcact tagaagttat acacaatata gaaatgatta tagcaatagg
     1861 gctgacaatt ataatagaag aagacgtaat gatttagatg gatgttgttg ctgttgctgt
     1921 gatgattgct gttgttgtct aggtgacgat tgttgtgaaa attgtgctaa gctttggtgt
     1981 ttagattcat gttgtgaatg ttttggtgga gatttaataa cttgttgtta ggatgataaa
     2041 tatgagtata aaagttgctt atggaggagt tttattagca ttaaatgtga tattgttgac
     2101 tcttacaaat attattcctg taaatacatt gtttattatg gggttagcat cacttttagt
     2161 atcaattgta ataatggaat ggggctttaa gagtggaatc gctttttata taggttctat
     2221 agttttagga tttatagtga tggcaagtaa gtctcaatgg attgtatatt cacttacctt
     2281 tggaatatat ggtattgtaa aatatcttat agaaaaagat aggtctatat atattgagta
     2341 ttttatgaaa ttagtatttg caaatataat gatacttata ttgtattttt tattaagaac
     2401 aatagtttat attccaataa acatatttat tataggttca tttgagatag cctttatagt
     2461 atatgactat gtttacagtt cttttatagg ttattacaat aataggttaa gaaagatgct
     2521 atttaaaaaa taatagttga tttttatatt taattttaat gaaattgtag tttggataag
     2581 tgtaaatgat atatctgaat atacattaaa gcataaagct aaatttaata tattcttagt
     2641 gcaacatata aaagctttga tttttagtca aagcttttat atgttagata taaatattag
     2701 atataatata agtttttaaa atagatagta taaattaaat atccactata tttacatctg
     2761 tagatagaga ataaataata tcttttcttt ctactattat ctcaacattt ttagaaattt
     2821 cctcatcaag tgatatttga gataaaagtt cctttgttgg atttattgct attggatttc
     2881 caactctttt tagcatattt atatcaccat tagtatctcc ataggcatac gagtttgata
     2941 aatctaaatc atactttaca acaaaatcat ttatagcatt attcttactt atagaatccc
     3001 acattggaat aactgtgcct gtaaatatat tattttcaaa gacataatca cttcctagaa
     3061 aatcggttac attatatttt tttgccattt tttcaacaag aaatccaggg ctaccagata
     3121 taaatataac tatgtgtcct tgatttagat gccactcaat ccttgaacgt gtgtatttat
     3181 aaactctatc tgcttttaat ttaattactt ggtcacttgt aaagttgata caggttttat
     3241 ctaatccttt tagtgaatca acataaaggt cacatatttc taatagataa tcatcataat
     3301 taccttgtct tttatcccaa tccataaaag tgtctctagc atgtttatac caattttttt
     3361 ggtcaattat atcgtattta attaattttt taaaatgttc aaccataaga gagtctctat
     3421 aaagagtacc atctatatca aaaaaagctg ctatattttt cataagacca cctcaattaa
     3481 tcatttcctt atattatatt ataaattgta tctaagttgt taaaatatat gtataataat
     3541 ttatatatta aaaaacaagt attttagagt ttaaaggatg tgactatatg aattttaatt
     3601 ttgcaaaggc tacaaattct agacttatgg gcagtttagg tcttataata aattggatag
     3661 atgatgaaaa caatcacttt tgtcaatatt ttttattaga tgcagaggga ttaggattag
     3721 cagattatgt gagtttaaat aacccaacac aagaagaagc ttatatggaa gaagaaagat
     3781 tgatgggagg atttggttca gatagagttg aattaacgaa ggatgaatct ttatttttag
     3841 tatcttattt tggtaataag aatctttatt atgataagct attgccagga gataaatgtg
     3901 agtatattga tattataaaa aactataaaa cagatttaat tatagaaaaa ttatacaata
     3961 aaatatgtaa aagagttgat gaggaagttg aatttataaa ctatatgact atgagattta
     4021 tagcatggga tagagaaagt ttaaaatatt tttctggaag tgacgagatt gcaaatatgc
     4081 atattactaa tataaatgga acattactta aaaacgtggt aagtgataaa ggtcaaggca
     4141 gatatataag tgaagcttta tatgaagaca atgatggata ttatatatgt aaaattgctt
     4201 tttgcataag taagtgctat gaaactggtt ttagaattaa ttctctatta gtgacagata
     4261 aagaacttat gtatgatttt gaggtatttg atgagatttc taaaagtgaa tttgtaagtg
     4321 tttacagtgt aaattcacct gaagaatttg caggagtatt ttatagagaa aatccatttc
     4381 tgttaaaaag taatatgaat gaaggtatat tttttactag atttaatttt aataatgacc
     4441 atgtaaaaga aaatgtatat ataataaaca atgatatgaa agctatatat tacttaatgg
     4501 gaaataagtt ttttattggt acttataacg aaaatgattg caattatatc aatgaaataa
     4561 gtcagtctaa ttatagtgac tatattaaat ttgaagagaa atttttcttt gagcaaaatg
     4621 cattatatga ctttgcagaa tcaggttcat tagattttga tgattttttg gaataataaa
     4681 tttagacatt atttaagcga gattaactga atttaatctc gcttaaattt atttc
//
LOCUS       DACTGW010000246         4714 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.249, whole genome shotgun sequence.
ACCESSION   DACTGW010000246 DACTGW010000000
VERSION     DACTGW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.249"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..713
                     /locus_tag="I9573_003246"
     CDS             <1..713
                     /locus_tag="I9573_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAT4797838.1"
                     /translation="KLSKFNLKHKYICHTSGSLKSSILFCSKKAGALIYSIHPIFAFS
                     NKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDGLGNQFFIRDKDTSSTYHLANVLVSN
                     LVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTKALTGPVSRGDITP
                     IRKHLSVLNKKDEDIYKILSLNLLKIIALGNDNSLKGTKNITIENAIENLISKSEKYK
                     EIYKLLGGKNDEKYHTDF"
     gene            688..1515
                     /gene="panB"
                     /locus_tag="I9573_003247"
     CDS             688..1515
                     /gene="panB"
                     /locus_tag="I9573_003247"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAT4797839.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGAKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIADMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGVFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1534..2382
                     /locus_tag="I9573_003248"
     CDS             1534..2382
                     /locus_tag="I9573_003248"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAT4797840.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNINTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PKNIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            2793..2975
                     /locus_tag="I9573_003249"
     CDS             2793..2975
                     /locus_tag="I9573_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797841.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            3807..>4714
                     /locus_tag="I9573_003250"
     CDS             3807..>4714
                     /locus_tag="I9573_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HAT4797842.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKDITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYK"
BASE COUNT         1869 a          590 c          680 g         1575 t
ORIGIN      
        1 aaaaactttc caaatttaat ttaaaacata aatacatttg ccacacaagt ggctctttaa
       61 aatctagtat attgttctgt tcaaaaaaag caggtgcatt aatttattct atccatccta
      121 tatttgcatt ttccaataaa aataccaaca taaaaaaaat gaaagacatt tgtttttcca
      181 tagaaggtga taatgaacaa gatgatttag ttattcaaaa tttcatagat ggtttgggaa
      241 accaattttt tataagagat aaggatactt catccactta tcatctagct aatgttttag
      301 tttcaaattt agtattatct ttactagata taggagtaag ttactttatt aaattaggtt
      361 tatctgaaga ggaatcttta aaagctatca gtcctttagt aaaaaaaaat atagaaaata
      421 tacttgataa tggctttact aaagctctta ctggtcctgt ttctcgtgga gatataacac
      481 caataaggaa acatctatct gtattgaata aaaaagatga ggatatctac aaaatactat
      541 ccttaaacct actaaaaata attgccttag gaaatgacaa ttcattaaaa ggtacaaaaa
      601 atattactat agaaaatgca atcgaaaatt taataagtaa atctgaaaaa tacaaagaaa
      661 tttacaaact tttaggagga aaaaacgatg aaaaatacca tacagacttt taagaacgct
      721 aaatatgaag gaaaaaaact atctatgctt actgcttatg actactcaat tgcaaaaata
      781 atggatgaat gtgatataaa tggtatttta ataggcgatt cattaggaat ggtcataaaa
      841 ggtgaagaaa acactctatc tgttactata gacgaaatta tatatcatac aaaagctgta
      901 aaaaatggtg caaaaaatgc cttaatcgta agtgatatgc cttttctatc ttaccatgtt
      961 tctatagaag atgcagttaa aaacgctggt agacttataa aagaaggagg agctcatgct
     1021 gtaaagttag aaggtggctc aaacgttata aagcaaatag aaagcattgt aaatgctcag
     1081 atacctgtaa tggggcattt aggattaact cctcaatcag taaattcatt tggtgggttt
     1141 aaggttcaag gaaataccag tgaaacagca agacaactta ttgaagatgc taaattaata
     1201 gaaaaagcag gtgcattctc tattgtcctt gaaggagttc ctacgaaaat agctgatatg
     1261 gtaactaatt ctatttcaat tcctacaata ggtatagggg caggaattaa ctgcgatgga
     1321 caaattttag tttatcaaga tatgttggga gtgtttggag attttgttcc taagtttgta
     1381 aagcagtatg caaatatagg agatataatg aaagattcaa ttaaaaatta catcctagaa
     1441 gttaatacag gtgcatttcc acaagaaaaa cacagctttt caataaatga atctgagtta
     1501 gaaaaattat actaatatta aggatggtac aaaatgcttg taaaagaaat taaattacta
     1561 agaaatataa tcaaagattg gagaaaacat ggctattcta tagggcttgt tacaacaatg
     1621 ggatttttac atgaaggaca ccaaagtctt ataaaaaaag ctgtaaaaga aaatgataaa
     1681 gtagtagtga gtgtatttgt aaatccaact caatttggac caaatgaaga ttttaatagc
     1741 tatccaagag atatagataa agattttaaa tattgtatgg atagtggagc aacagtagtc
     1801 tttaacccat caccagaaga aatgtattta aaaggtaatt gtactaccat taatgtttct
     1861 ggacttacag attttctttg tggagctaaa agacctgttc attttggagg tgtctgccta
     1921 gttgtttcaa agtttctaaa tatagtaaca cctgataaag cttattttgg agagaaggat
     1981 gcccaacaat tggctgtaat taaaagaatg gtcaaggatt taaacataaa tacagaaata
     2041 ataggctgtc ctataattcg tgaaaatgat ggattagcaa aaagttcgag aaatacatat
     2101 ctctctgagg aagaacgaaa atctgctcta atattaaata agagcttaag tttagcaaaa
     2161 gaagaattag taaaaggcaa tctaaatcca aagaatataa aagaattaat aacagctaaa
     2221 ataaattctg aacacttagc aaaaatagac tatgttgaaa ttgtagatag tgagacatta
     2281 caacctgtta agcaaataga acattctatc cttgtggcaa ttgctgtgtt tataggaaaa
     2341 accagactaa ttgataattt tacatttgag ttaaatatat agtatataat tttatataat
     2401 atatctaaaa aataatagaa tacctaaaaa gtaataaatt acctaaaaaa taataaattg
     2461 cttagaaaat aatagattac aataactaaa attttaaaat taaattcctt aaaagtatga
     2521 ttaattataa ttatataagt atattcttat tacacttata ttaatttata ttaaatatct
     2581 actaactcaa atattcataa ttaaacttta aactggataa attttctttt aagaatgaaa
     2641 gtttatccag ttttgcgatt ataataacca gttttacata tccacttgta aaatatatct
     2701 aagcaatata tcatactata tagatttatg agtgaaataa tatctgctat ttactgaata
     2761 tttttatttt tcgtttttta gggggattag ttgtgtttaa agataagatt gatgaatgtg
     2821 ttcatataat gactgcatat attgctagtt taaaagaata ctattcattc atagaaactc
     2881 aaattggtga ttttattaaa aaatatggag aagatgttgt agagttatgt ctccatagag
     2941 tgatgattct actatgcgaa tgtggtttgg cataaaatta aatttattaa aatgggtaag
     3001 aagaatccta tataatattc ttcttaccca ttttaatata tcaatttcat agcttatcgt
     3061 ttaaaatatt ttacctccat tactagacaa ctttacattc tctttttaat tctagtatat
     3121 ttaaatcatt ttagttgctc cttatttatt cttattactt gttttatttt ataaataaaa
     3181 taattctttt taataatgtt ttttgttgat attcctatca cacatccaat tatttatcat
     3241 tgtcttgaaa ttgtttgcat acttaattta atccaattga aacaccccag catagaatcc
     3301 cctgtaaaat agaagttgga ttaaatccta acatccatac agaaaatact actccaagca
     3361 caagcaatat aatggaatat atttatcact taatttttta tactttttaa atcccatacc
     3421 taaaacatta agtgcagcta ttagaattaa taattgttgt ggtataaaat tatgattgct
     3481 tcatatttat tcattttttg ctataaaagt tcttgtttca caaaaaatat ttgttttgta
     3541 tcaaaataat acttataaat tacataattc tcataaaaat ttctagtatt acttgtgctc
     3601 ttaataaata atagccaaca agtaccttaa atcattatat ctatatctaa taagaaacac
     3661 agtaagataa ttttttcgcc cataacttaa taaaaactgt tgatattagt agcaattgaa
     3721 tttagttaat ttatttataa tatacataaa tttaaatatt acgaataaag tattataaat
     3781 aggatacttt ctaaagggag gaaattatga tagataatca aaaatatgtt attttatcac
     3841 tagaattaca tttatttttt tcaagaatta tgaaagagca tgctcttttt ttagaagcag
     3901 gattcacaaa taaaaattat aatcttgcta tggaagctga ccactataaa aagcaatttg
     3961 aagatttatt atcatacact gttagtgcta gtaatggtat aattagacct gatatattat
     4021 attcagaaga gcttgtaact actctcacat cagttgcaga acaaaaaaca gaagagttta
     4081 cagggataga aataaacaaa gacatcacta caagagaatt aaatctacaa agtggtgtaa
     4141 acccacaagt tggtcaagac ttagtgaact atgtagctca acttaactct gatgcaataa
     4201 gattacttga tgggcttatt aattttaaag aaagagtctt agatggtgta ctatcatgta
     4261 ctatatttac atcaaactac cctctacttc ttgaacatat aatacatgaa gcaaatttat
     4321 atcgttctta tgtagttgac cttgaaaata aaatagatat tgagtcaaaa aacgctaaag
     4381 aaatagaatt attctgggac catattatga tggaacatgc tctgtttatg agaggattac
     4441 tagacccctc agaaggtgaa ctaataaata cttcaaatga ttttgctata aaatttaatg
     4501 aattaattga aaaaacaaac gaaatgactg attctaatat caagaacatt acagaagaaa
     4561 ctctaaatga aactgttgag tttaaagatt ttaaagaagc aggagcatca ggaatagaac
     4621 agtgtaagat aaaatctata atattaccac ttttagcaga ccatgtttta agagaggcaa
     4681 atcattatat tagaatattg gagagttata aaaa
//
LOCUS       DACTGW010000247         4670 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.250, whole genome shotgun sequence.
ACCESSION   DACTGW010000247 DACTGW010000000
VERSION     DACTGW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.250"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            300..1217
                     /locus_tag="I9573_003251"
     CDS             300..1217
                     /locus_tag="I9573_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAT4797843.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVNQ
                     GEELIELSKKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKHFSVNTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(1346..2275)
                     /gene="pfkB"
                     /locus_tag="I9573_003252"
     CDS             complement(1346..2275)
                     /gene="pfkB"
                     /locus_tag="I9573_003252"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAT4797844.1"
                     /translation="MITVITFNPSIDRMYKVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEYNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            2544..3269
                     /locus_tag="I9573_003253"
     CDS             2544..3269
                     /locus_tag="I9573_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4797845.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRIQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            3352..4506
                     /gene="nagA"
                     /locus_tag="I9573_003254"
     CDS             3352..4506
                     /gene="nagA"
                     /locus_tag="I9573_003254"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAT4797846.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQRKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFSVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYVPALSCNIDNVCGSIHQGMDADFIVTDKNFNIYETYISGICMYKVNK"
BASE COUNT         1742 a          626 c          663 g         1639 t
ORIGIN      
        1 ctttaaaaat ttaataaaat catactttag tatttttatt ttacgataaa aacttgttca
       61 gaccaaagtg gtaattacca ctaatccaaa tataaattaa tttgaatttt ttgtcaatac
      121 ataaaatatt tatatcaatc aattttatag atatcctaca aaattacttt gtcaatattt
      181 cttatttcgt taatttactt atataagctt taaatttatt ttttgataca aagtttacaa
      241 atttatgtgt ctgaattata atcaattctg taaatataaa aaccttagga gaaagtatta
      301 tgaaaaatat aaaaatggga atgataggtc ttggctccat agctcaaaag gcatatttac
      361 ctatattaac taagtctgag agatttgagt ttgtaggagc ttttactcca aataaagtta
      421 aaagggaaaa aatctgtagt gattatagaa ttatgccatt cgattctatt gaatccctag
      481 ctaaaaaatg tgattgtatt tttctacata gctctacaga aacacactac gaaattataa
      541 aaatattatt aaatttaggt gttcatgtat atgtagacaa acctcttgct tcaacagtaa
      601 atcaaggaga agaacttatc gagttaagta agaaaaaaaa cttaaattta atggttggat
      661 tcaaccgtag attctgtcct atgtacaaag aaattaaaaa taatgctaca gaaatcgtat
      721 ctatcaatat atgtaaacat ggattaaatt ccttaagaaa tgttagattc gacagtaccc
      781 ttatagatga ctatatacat gttattgaca ctgctttatg gttagcaaat gaagatgttg
      841 aaattagcgg agaagattta tttttaacag acaataagaa tcttatattt gtaagtcata
      901 aacttaaagg taaacatttc tctgtaaaca cctctatgca tagagattct ggaacaaagt
      961 tagaacaagt tgaaattctt tcaaaaggta agattcaaag agttaagaat cttaatgtac
     1021 tggaaataga agaaggtgga aacctaactt taaaacaaag tggtgcatgg gtaaatatat
     1081 taaaacaaaa gggttttgag gatatatcaa atcattttat tgattgtata gaaaataata
     1141 taaaaccagc tattaatgga gaagagtgta tcaaggctca aagattacta gagaaaatta
     1201 taaattcagt aaaatagata tttattataa aaataggtta aaaatgaatt tttctaacct
     1261 atttttatat aagactatat cttttatatt aaaattaaaa tctattacaa caacattaaa
     1321 tttattatat tcccattaaa tttatttaac ttaacttttt tacttcaact aaatcttgat
     1381 atttatcaat ttctcccaaa tctacaaatc cagtttccat atttaaagca ttacttatac
     1441 cgcaagcatt tgcaaattta agtaaatttt ctattttata accccttaaa atacctacac
     1501 taaatccagc cactgtgcta tctccactac ctacagtatt tatacacttt acaactggta
     1561 cttttacatc atatacatta tattcgttta aatacaccat accatctttt cctaaagata
     1621 tacatacatt ctctgcaccc atttcaataa gtttttttcc tgacaagata atttcctctc
     1681 ttgattctat atttattcct aataaatgtc ttatttcatc tatatttggc tttattaaat
     1741 atggttttga ttttaaagct atttttaatg cttctcctga tgtatctaat ataaatttta
     1801 tattatttgc tctacatata tttcctatct tttcataata atctatcggc atattctcac
     1861 aataactacc agaagctact agtattttag tattttttag tacttcctct atatcatttt
     1921 caaatctctc taaatcgctt ttttctacta ttggtccttt ttctaaaaat tctgtactaa
     1981 ctttattgtc ctcaattatg tttaagcaat ttctagtttc ctgctctatt tttgtaaatt
     2041 tattttgtat atctagtttt ctaagttcat tttttataaa ttctccatta aatccaccta
     2101 gaaatcccat agctaaagga ttttctttta aaattttaca taccttagct acgtttaatc
     2161 ctttacctcc agcagttgca ttcttactta ctaccctttg cacctcacct atgtttatat
     2221 tattcacttt atacattcta tctatagatg ggttgaatgt tattactgta atcatactag
     2281 attctcctca ttattaattt ttacttttct aaacaataaa ttaatctgta aaataaaaaa
     2341 tacctcatct ataatatacc caaagtggtt attaccaatt tgaatataaa ttattttact
     2401 tgttttgtca atataccata attttataat aaattaatag ccttacttat tgaattttga
     2461 ttaaacttta ttcattgaca taaataattc tatattctat aatcaaattg tggtaataac
     2521 cacttagaaa ggactatgtt tttatgataa aaagaaatga taaaagacca atatatgacc
     2581 agcttgttga gatattaaga actaagattg aaaatgagat ggaaccaaac gatagaatgc
     2641 tttctgaaag aaaaatttgt gatgaatatg gagtaagtag aaccacagtt agactagcta
     2701 tggctgagtt ggaacatatg ggatatatat acaaaagaca tggaaaagga acttttgttg
     2761 ctgcactaag tcaaaattct caaaacttaa tggaaagtta tagctttaca gattatatga
     2821 gaatacaagg aaaaaagcca ggtacaaagg tattatcatt tgaggtagta gaaagtacaa
     2881 agtatttctc tgaaaaatta ggtataactc ctggtgaaaa aatgataaaa ataatgcgtt
     2941 taagacttgc tgatgacctt cctatgatgc ttgaaagaac atatcttcct atgaaagaat
     3001 ttataggatt gacaaaagaa aaaatcgaac aaaaaccttt atatgaaata tttagagaag
     3061 attatggcga gattattaaa gttgctgatg aagaattttc agctggaata atctctgata
     3121 aggaagctaa gttattggaa gtaccaggag attcaccttg tctgaaatta cttcgtacca
     3181 catataatga tgacaatata gttattgaat ttactttgag tgttgctaga agcgataaat
     3241 ttgtatatag agtaagacat gtaagatagt gaattaaata gctttcaaga aaatagaatg
     3301 atattataaa cgtcaatgcc acaatgatag tataattata aggaggttat tttgaaaagt
     3361 tttatttatg cagataaatt ttttatgaaa catggaatca aagaaaaagg ttaccttagt
     3421 ataattaatg gcaaattcgg tgaatttcaa caagaaagac ctaatattac tgataatgtc
     3481 atagattttt caggtaaata tattgctcct ggcttagttg atactcacat tcacggtctt
     3541 ttaggtgcag atgtcatgga caatacattt gaagcaataa acacaatttc aaaaggctta
     3601 ttaaaatatg gagtaacatc tttcctacct acaactttaa ctgattctgt agatacacta
     3661 agtgattctg tagaaaatat aagcaatagc ttcaatcgag ttgagggtgc aaaaatacaa
     3721 gggatttatt tagaaggtcc attttttaca gaaaaatata agggcgctca aaatgaaaaa
     3781 tattttaaag accctgacat caatatatta aaattatggc aaagaaaatc gaaaaattta
     3841 ataagaaaaa tagcaattgc tccagaacgt aaaggttcaa tagaattaac aagatatgca
     3901 actaaaaata atatctctgt ttctcttggc catagttcag ctacatttga acaagttaaa
     3961 caagtcgtta attcaggtgc taaagtgttt gtacatacat ataatgggat gagcccttta
     4021 aatcatagag aacctggaat ggttggggct gctatggtac taaaagatac ttatgctgaa
     4081 ttaatttgtg atggtcatca tgtaagccca atagctgtta aaataatgat ggatgcgaag
     4141 tctagtaata acatagcttt aattacagat tgtatgaggg ctggtgctat ggaggatgga
     4201 aaatatactc ttggtaagtt tagtgtaaat gtaaaaaata gtgttgcaag attaaatagt
     4261 gggagtttag ctggaagtgt attaactatg gacaaggctg tgagaaatat agttgattgg
     4321 aacatagcca ctttagaaga tgcaataaaa atggctacat atgttcctgc tttaagttgt
     4381 aatattgata atgtctgtgg ctccatacat caaggtatgg atgcagactt tatagtaaca
     4441 gataagaact ttaatatata cgaaacttat ataagtggta tatgtatgta caaagttaat
     4501 aaatagattc aatataaagt tatggaggct tcctaaaaat agagataagt ctctttttca
     4561 ttgttaaaat gatgaaaata catttgattt catacaaaaa gagtgcctca taaactaaaa
     4621 agttaatttt gagacactcc ttttattaag gttctttatt ttacaatata
//
LOCUS       DACTGW010000248         4655 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.251, whole genome shotgun sequence.
ACCESSION   DACTGW010000248 DACTGW010000000
VERSION     DACTGW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.251"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..259)
                     /locus_tag="I9573_003255"
     CDS             complement(<1..259)
                     /locus_tag="I9573_003255"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAT4797847.1"
                     /translation="MVFETFDKVIKLHPNAKPVFHSDRGFQYTSKVFESKLLAQGMIQ
                     SMSRAGRCIDNAPMEGIWGILKVEMYYLRKFDTYEQLVKIIE"
     gene            355..848
                     /locus_tag="I9573_003256"
                     /pseudo
     CDS             355..848
                     /locus_tag="I9573_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1390..2745
                     /locus_tag="I9573_003257"
     CDS             1390..2745
                     /locus_tag="I9573_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT4797848.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATVLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(2863..3531)
                     /locus_tag="I9573_003258"
     CDS             complement(2863..3531)
                     /locus_tag="I9573_003258"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HAT4797849.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(3598..4026)
                     /locus_tag="I9573_003259"
     CDS             complement(3598..4026)
                     /locus_tag="I9573_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAT4797850.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            4133..>4655
                     /locus_tag="I9573_003260"
     CDS             4133..>4655
                     /locus_tag="I9573_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797851.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENATKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVS"
BASE COUNT         1633 a          683 c          555 g         1784 t
ORIGIN      
        1 tctctattat tttaactaat tgttcatatg tatcaaattt ccttaaatag tacatttcca
       61 cctttaaaat tccccaaatt ccttccatag gagcattgtc aatacaacga ccagctcttg
      121 acatactttg tatcatacct tgtgctaaaa gtttcgattc aaacacctta ctagtatact
      181 gaaaacctct atcactatga aaaacaggtt ttgcatttgg atgtagtttt attactttat
      241 caaaagtttc gaataccaag ttattattat tcgattttcc aataacatat gaaattaata
      301 gatataacgc cttctatttt acagtcttat ataaataaca tatacaatga gcacatgtta
      361 aattcaataa aaaaccaagt cgattttata aaatcagtat tgaaagaatc ttatagatta
      421 aaagaaatat cagaaaatat ttgtgatttt gttacaaccc caatcaaaaa aaatagttca
      481 acttctaagt tttatacaaa gcaagaaact caattattat tagaaaaatc aaaaaatatt
      541 cctataggta taccaatttt tttgatacta actcttggat tacgttttgg tgaagctgta
      601 tcacttatat ggtcagatgt agaccttgat aaaaaatctc acatgtaaat caaacattag
      661 tttatgtata caataaaata atcttcaaag accctaaaat ccctagatcc aaaagaaaat
      721 tatttgctcc tgatgaactt ataagtttgc ttaaagggca aaaattaaaa caaaataagt
      781 taaaattaca gggtatattg aaaaatgaat ttgatttgat atgcttaaat aaaagattca
      841 acccgtgagt tcaaccaact tttgttgttc attttaaaaa actattaaga gataataatc
      901 ctagagatat aaaagtacat gaattaatac atgcaaatgc gactttgata ttactttcag
      961 gtactaacat aaaagctatt tataaaatat tagtacatgc agatataaaa atagctatga
     1021 ataaatattc gatattctca cttattagat gaaatggata aagaaacatc tgaaaatata
     1081 agcaaaatac tattcaaata atgttcagtt ttatgtcaat taaatgtcac tcaaagtgct
     1141 ttttttccat ttgtcagtta aatgtcactt aaagtacctt tttcctattt gtcagttaaa
     1201 tgtcagtaat atataaataa gatatattta taagaccctt aaaatcacta ttatttaata
     1261 aaatttataa ttatatacta tttgctgttg acattggtat tataccatag tttatcattt
     1321 atattagaaa taaaattatt aattaatagt ttcgtaattt tataatagat ttacagggag
     1381 tgataataaa tggatacaaa aatatctata aacgaaaaca tgagtctagg taaaagattt
     1441 tttaaatacc tagcaccatc tgtagttgca atgtgggttt tctcactata tacaatggtt
     1501 gatggaatat ttgtaagtaa aggagttgga gaattggctt tagctgctgt caatatatca
     1561 atgcctttta ttaattttat atttgcagtt tctctattat tttcaactgg tgcatctacc
     1621 ataattgcaa tctatctggg gaaaaaagat attaaatctg ctaacgaagt cttttcattt
     1681 aacttagtat caataattat attatcaata attatattag ctataacttt ttttaattta
     1741 gatagattag cactattttt aggagctaca gaatctacaa ttggtatggt taaagattat
     1801 ttaggaataa taatattttt taacggattc tttatagtgt cttattcttt agaagttata
     1861 ataaaaactg atggctttcc aatattagct actgttggtg taataatatc tgctcttaca
     1921 aacatcatac ttgattactt gtttgtaatt gaatttggat ggggtgtaaa aggtgctggt
     1981 attgcaactg ttttgtctca agtcttttct actattttct tcttaataca ttttttaaga
     2041 aaaaactcta ctttgaattt ttcaaaattt agaattgatt ttaagacact tagaaaaatt
     2101 gtatttatag gttttcctga ttcaactact gaattaagtt gtggtatagt tgttttactt
     2161 tttaacttaa gtcttactaa atatattgga gaaaatgctc ttatttatta tagtgtcata
     2221 aattatataa atacattagt acttatgact atgatgggta taactcaagg tatgcaacca
     2281 cttactagct tttattatgg tgcaggaaat atagataatg taaaaaaatt attaaaaatg
     2341 gggataaaag ccacaataat agcatcagtt gctgtatttg caatttgtat ggctttttca
     2401 ggaccaatag tctctttatt tatacaccca gaagaaacaa tgctattcaa tgaaggagtt
     2461 cgtgtattta aaatattctc tatatctttc ttattggtag gaattaatgt aataatttct
     2521 ggattctttg tatcagtaga aaaaccatca atttctactg ttatatctct tggtagaggg
     2581 cttgtaatag ttgttttaag tctaatttca atgatactaa tatttggtgg acaaggaata
     2641 tggatgacta ctattgtttc tgagtttata tgtcttattt tatcattagt ttttctaaaa
     2701 aagaattttt ctacattaga ctctaatcta aataaggttg cttagatgat aagagtagca
     2761 atatatgaaa atatctaaat aaaaaatacc tattatacaa tcttgcaaat attagacctt
     2821 tgcaaaaatt aggattatat aataggtatt ttatttatgt aattatttta tttctaaata
     2881 tccatagcca tcttttatta aagttccttt tacttcatat tctacaggtt ttttaaatat
     2941 tggaccatct acgacaaatg tatgatactc tccatgttct ccacatgggt ctgcaccagc
     3001 attttttatc ttctccacaa gttctctagt taattttttt cctaaaaatt cctctcctaa
     3061 gtgctttaag tttacttttg ttacaacact acaaaatcca ctgtctataa attcatatac
     3121 taaatcctct ctactctctt gccataatgg taaatctact ttcataccaa ctgcttcaca
     3181 tctatcagta tcccattttc tatgactttc tatatctata tctccatatg cacatatcgt
     3241 tgctcccatg tttttagctt ttattaaagc cctctcaaag ctctcttcat atccttctac
     3301 accacactca gcaactaata aaggtaatcc tatattgcta cttacttgct taagcatatt
     3361 atattctaaa ttatgtgtcc atgactttcc atgttcttca cttatagttg tcaagagtgc
     3421 cacaggctta tatccttttt tcaacattcg atttaatgct aatatactat ccttaccacc
     3481 actaaatgac attacaaatt tttcatcagc caattttaac acctctttca tctaactttg
     3541 ttttattaac ataattatac caaatttttg agttgcatag caaattatat taaattgtta
     3601 attcactata aagaatgtcc ctaatattaa aagtataacc cctatgtatt tgtataaatg
     3661 cattgtttct tgtcccaaaa ttacgctatc aataaaaaat ccaactaaaa cttgggatac
     3721 aagtgttaat ataagtgtat ttgatactcc taaatttgga attgccttta ctgtaaaata
     3781 tattattaaa cctccaaata ctcctcctaa taacaactta taattaaaac catttataga
     3841 tactagacac tttaagtcac ctgtaactat tatacttgcc aaaaaaaata ttgctcctac
     3901 agttaaactt ataaaggtgg ccactaatgc tgttgtactt tttcctagtt ctccatttat
     3961 aaaagcttca agagctgtag aaccacctgc taataatgca aatattattg cccataattt
     4021 tgtcatggac aaacctcctt ctatataatt ctattaatag aagtctatga tacaacctat
     4081 taattaatta ctaagtatag tttaattttt atacactagg ctttatttgg atatgaattt
     4141 ctgttataat tgttctatcg attttggagg tgattttatg tacacaaatt ttaatatcga
     4201 ttttaataac tttaataaaa aagaaaatgc tactaaattt atgttaatgg gagtattgct
     4261 cattatctta ggattattat gccttacatt taagacatta ggtataaaat taatttcttg
     4321 gacttttggt attgcacttt tattctttgc atatttaaat ttaaaaaata ttaatgaact
     4381 aaaaagatat gctactaaag aagaaattaa accctctatt aatatccaat gggttctaat
     4441 aattgcatgt atattacttt ttgtatttcc tcaaaaaata caaagcatat tttcattatt
     4501 tttagggttt tatttaatct ttaatcaact agtagcactt gtaaatagta agaataatcc
     4561 ttattctaaa tttacaactt ggaatatagt taagattcta tttggtatat gccttatatt
     4621 atctccactt tttttatcta gatttatagt atcga
//
LOCUS       DACTGW010000249         4653 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.252, whole genome shotgun sequence.
ACCESSION   DACTGW010000249 DACTGW010000000
VERSION     DACTGW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.252"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            1..660
                     /locus_tag="I9573_003261"
                     /pseudo
     CDS             1..660
                     /locus_tag="I9573_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(879..1916)
                     /locus_tag="I9573_003262"
     CDS             complement(879..1916)
                     /locus_tag="I9573_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HAT4797852.1"
                     /translation="MNKKLIKAKIFIWSILAISLSIVFISLLIGTIISHSFNFPFNTL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVRIKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDGNSLSIYDNSTKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNTVEANTISGAIGLSGNINKINLDTTSGHIDITKLNSKNATLKSTSGEIQLSGKID
                     VLNLETTNGNINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKINSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRHSDLYTYKNGITNLYLSTT
                     NGNLTIIKNNH"
     gene            complement(1909..2595)
                     /locus_tag="I9573_003263"
     CDS             complement(1909..2595)
                     /locus_tag="I9573_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797853.1"
                     /translation="MLDKIRTYIDSSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFMNAYWIIVVSIYLITSILFNIWSFSWILFIIGAAGVNIVKGIIQL
                     KGYKEEDKYE"
     gene            complement(2607..2927)
                     /locus_tag="I9573_003264"
     CDS             complement(2607..2927)
                     /locus_tag="I9573_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4797854.1"
                     /translation="MIIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITNIGKEKYIKSREEWNNTKALI
                     DILI"
     gene            3288..4628
                     /locus_tag="I9573_003265"
     CDS             3288..4628
                     /locus_tag="I9573_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAT4797855.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLFLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         1713 a          513 c          683 g         1744 t
ORIGIN      
        1 tttggctgaa gatgaagaaa ataatcatat aaatattgag atgcaaagag cttttgagaa
       61 cgattatttg gaaagagctg aatattatct tagtcgtgta catggtagaa agttagaaga
      121 aggtaaagag tataaagaaa ttggaaaaac tattggaata catatactaa atcatgtaaa
      181 atataatcac atagaagatt atgtaaattg cctgagatta acaatggacg agcatccaga
      241 tatttattct agtaaaactg cattatattt tatagagctt ccaaaaattc ataaatctga
      301 ctatatagta aatagaatta tgctatgggg aaaacttatt gataatcctt ctagctcaga
      361 tgtaagagtt atagctaaga atgattctat tattaaaaaa gcacttgata gattaaaaga
      421 attaggtagc aatgaagaat atttattaaa tctgaaaaga ggtgcttata tcatgaataa
      481 aagtagaaat tttaaagaag agattgaaag agagacagca attagagttg caaaagaaaa
      541 agattataaa ataattataa tgttgaaaaa aatggtttta aattagatga catattgaat
      601 aaatttaatg attttgactt aagtgaagat gagataaaag aagtatatga aaataattga
      661 tgataaataa aaagtgaact tatataagtt tgctgaatct gagcctggaa atatttatgt
      721 gtatttttta atgaataaaa atttatttta aaaacaataa ttagaggtac agttaattat
      781 atttttatga tactgtactt ctagtttttt ctattgtaga tttattattt actaataaat
      841 ctacaataga agtgcttttg ttgtaagaat cattttatct aatgattgtt ttttataata
      901 gttaaatttc catttgtagt gcttaaatat aaatttgtaa taccattttt ataagtgtat
      961 aaatcactat gacgattcaa ctcaaagtca ctgtccaaaa caccagaaac cttatcaaaa
     1021 tttaaagtga aacctggatt atcaggaata gacaaatcga cattggcaga agtagtttca
     1081 cagtacagag aagacaacat tttgctagaa ttgattttta tattactgct agttgactca
     1141 gctttaatgt cagaaaaaac tccagataag ttcatttttc cagaaactga actacaagta
     1201 agatttttat tgtatatatc ttttaaattt atattgccat tagttgtttc taagtttaaa
     1261 acatcaattt tcccagaaag ctgtatttca ccagatgttg atttgagtgt agcattttta
     1321 ctatttagtt ttgttatatc aatgtgacca ctagttgtgt ctaaatttat tttattaata
     1381 ttaccagaga gacctattgc acctgaaatt gtgttagctt caactgtatt acttcttatg
     1441 taagtgctat atatatctgc tgaagtagta tataaggtta ttttatcata aagcttattg
     1501 ggcaaatata gttcaagtat tgttggtttt ggaagattaa ttccaatgaa gaatttttta
     1561 gttgaattat catatataga aagagaattt ccgtcaacca ttatttttgc tagttttttc
     1621 tttgagaaat tactagggat tttttgaatt aatttgattt tattagaatc acttctctta
     1681 attctaactt cacctgaaat ccaatcaaca ttaatgtcat ttattttatc tgaaaaactt
     1741 tcatttttag ccactatata gttatcataa ttgtatttag gattaagagt attaaaaggg
     1801 aaattaaaag aatgtgaaat tatagttcct ataaggagtg agataaatac aatagaaagt
     1861 gatattgcca ggatactcca gatgaaaatc tttgccttta taagcttttt attcatattt
     1921 atcctcctcc ttgtagcctt ttagttgtat aattcctttg acaatgttga caccagcagc
     1981 acctattata aatagtatcc acgaaaagga ccaaatatta aatagtatgc ttgtgattag
     2041 atatatagaa actactataa tccaatatgc attcataaat gatttataaa ctggatttat
     2101 atgattataa ttttcctcaa aatcctcaaa ttctttgaat ttatttgata aattatcatt
     2161 gtatagagca tagtttgtcc ttgtcatttg attatagact aataaaccta ctccaagtgc
     2221 tattaatatg agcattaaaa gaatagctat agttggttct atgccaaaac ttacaactga
     2281 gactacaaat aggaaagggc ttattataaa aagagcaact gctattgatg ctcttaaggc
     2341 tcttttcttt ttttcttcaa ttaattcaga aggtagagaa tatggttcct taacactttc
     2401 aattaactca gataaatcac caattcctga gattactgag ttatatgctt cttgttctgt
     2461 tttaccatct tgtatttggt cgttatactt ttcttttaga ttttcaaata attcatcttt
     2521 aagttctttg actttttttg tctcattaac tcctgtaaag ctactgtcaa tataagtacg
     2581 tattttgtct aacatatttt cacctcctaa attaatatat ctatcaatgc ttttgtatta
     2641 ttccattctt cacggctttt tatatatttc tctttaccta tgtttgttat tgaataatac
     2701 tttcttctag ctccagtaac ttcatcaccc caataagagg ttattgcacc agattgttca
     2761 agtctacgaa atgctgtata caaagtagct tctttcaact catacaatcc tttggttttt
     2821 tcaagtatag atttattaat ctcataacca taactgtctt tttcaagtaa atgttttaag
     2881 ataattgttt ctgtatgacc tcttatcatg tcagatgcga ttatcatgaa aatcacctcc
     2941 aattaaatat ataatacttt gatatacttc gcctgtcaag gtatatcatg agaaaagaca
     3001 ttttattgta atatttataa ggttttatat agaaataatg taaaaacttt ataaataata
     3061 tattttatac ctagttatag ataactttat atagttatta ttaaaaaaag caataaaaag
     3121 tttaattcaa tttagcaaat ttagtgatat aattaaaaaa gaaaagaaat atggacttat
     3181 ttggaataat tttgacaatc tatttactac acgtgtagag tcgtatataa ttaaattata
     3241 cataaaatat aaaaaaataa tttagtaata cgttaggaga aagtattatg ataaagtcaa
     3301 gcttattttg gaatattttc attgcgatat cctttatgat agaatggttt ctttgtaaga
     3361 aaatgctaga ttatacaagt gagaaaagga tttctaacaa aaaaataaac ttttgtatgg
     3421 taggtattat tgtttttgct gacataatat atttttttga tgtacctcca aatataagag
     3481 taattatatc tatgataatt actattgcat tttataaaat ctcatatgat gtgggattta
     3541 taaagtctgt attaatatcc ttaatttatt ggatgttact aatcgccaca gatgctttga
     3601 gtatatcttt aacagtttgg cttaattcta taaatgacat gagtagtatg gggacgggag
     3661 atatatacag aattgagtct ataatattgg gaaagatact tttgattgtg tctttgtttt
     3721 tatatagagc cttaaagata aaacttaata tagaaataaa taaaaggaca ttattttata
     3781 tagttattcc tatagtcgct aatatagcat ctttttttgt aatttttgaa tacatattta
     3841 atccagggtc tggtagctta atacataatt tccaattcgt tatgatttct gttgtattgc
     3901 ttatatcaag tatcacttta gttttaattg ctaaaaaatt acagcaatat aatactttag
     3961 ttgtagaaaa caatatgatt aaaaatactt tgaagtatga aaatgagtat tatgaagata
     4021 tagaaaagaa atatttaaaa actagattac taagtcatga tatgaaaaat cacatactgt
     4081 gtataaatgc tatgattaaa aaaggtattg atgttacttc ttatttaaat aatttgcagg
     4141 tagaaattca gagtaatgac ttgttattta atactggtaa ttttatatta gatgctatat
     4201 taagtgaaaa gaaagaagtt tgtgataaga atgatataga actaaaaata ggaataaatt
     4261 ttagttgttg tgaagctgtt gaattagtag atatatgtca tatattttca aatattttag
     4321 acaatgcaat tgaggcatgt aataaaattg atagtggaaa tagagttatt tgtgttaaag
     4381 gtgatgttat gcaaaatttt tatttaataa gagttgaaaa tactaagatg aataagatta
     4441 ttactaaaaa tggagatatt ttgacagaca aaaaaggttc tcttcatgga cttggaataa
     4501 aaagtgtaaa aaactctgta aataagtatg gtggtaatgt tgtaattgaa tatgatgata
     4561 aaaaatttgt agttaaaatt gctattccac ttagaaatgt gaagaaagca gttaatcatg
     4621 tgatttaaat gtatataaat ttgatgtgta gaa
//
LOCUS       DACTGW010000250         4623 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.253, whole genome shotgun sequence.
ACCESSION   DACTGW010000250 DACTGW010000000
VERSION     DACTGW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.253"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     regulatory      247..351
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            561..3035
                     /locus_tag="I9573_003266"
     CDS             561..3035
                     /locus_tag="I9573_003266"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HAT4797856.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHHETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            3035..4492
                     /locus_tag="I9573_003267"
     CDS             3035..4492
                     /locus_tag="I9573_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase subunit 2"
                     /protein_id="HAT4797857.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPLIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         1855 a          504 c          874 g         1390 t
ORIGIN      
        1 atgttatagt aaatctcaat ataaatttac atttggttct ttgtgtgtta ttttaataat
       61 aaattaagtg aaaatgttat tttacattaa tatataatat ctttgcataa gagtgaataa
      121 tataaaagag aaagaattga taataaaaac ttaacaaaaa aattaataca taaaaaaaaa
      181 taaatataat gtgttgacat ttaattgaaa tattcataca ataaaattga aaataaatat
      241 ttccaaatga gattagcgag agagtccttt atacgaaaag gcgccgaaga agaaatttaa
      301 gaaatgccaa tttttagaga agctttcagg caaaaatatc gctaattgat gggactctgg
      361 agagacttaa gttaagatac tataagtcac cgaagaagca aaataatatt aaattattaa
      421 atctttcagg tatacaaaca gggatatata ggctaggatt attttccatg ctgatatatc
      481 ttttttttat gaaaaagtac aggaaataat atcaaagtta cttaaataca gtaggtctaa
      541 ttttctaggg agggttatat atgaatgaat tgaaaagagt atctttgtac aatatacaca
      601 aagagcttgg agcaaaactt gttgaatttg ctggctggga aatgccttta gaatatgagg
      661 gtataaataa agagcatgag aaggttagaa aaagtgcagg tatatttgat gtatctcata
      721 tgggagaggt acagataaag ggagcagagt cagaaaaatt tatacaaaac ctagttacaa
      781 atgatataag cacattaaag ataaatgaca ttatatatac acctatgtgt tatgaaaatg
      841 gtggagtagt tgatgattta ttaatatata aatttggtga agaagattat ttattagtaa
      901 taaatgcagg taatatagat aaagatgtag catggataat aaaacaaagt gaaggataca
      961 atgtagatat aaaaaatata tctagtgaag tgagtcaatt agcaattcaa ggacctaaag
     1021 cagaagaaat tcttcaaaaa atcacagata tagacttaaa ctcaataaaa ttttataagt
     1081 caattccatc aacaaaagta tgtggttgtc cttgtttagt ttcaagaaca ggatatactg
     1141 gggaagatgg gtttgagata tactgtaaaa ataaatatgt agaaataatt tggaatgaag
     1201 ttctaaaggt tggaggagaa gatatatgtc cagctggtct aggttgtagg gatactttga
     1261 gatttgaagc agctctacct ctatatggtc atgaaataaa tgaacacata tctccaattg
     1321 aaggtggttt atctatattt gtgaaaacaa ataaagaatc atttattggt aaatcaattt
     1381 tatcaaaaga aaaagaaagt ggagctaaaa gaaagttagt aggatttgaa atgcaaggta
     1441 aaggaatgcc tagaaatgga tatgatataa ggattggtga taaaacagtt ggatttgtaa
     1501 caacaggatg tgcatctcca acgacaggaa aaattttagg tatgggaatt atagattctg
     1561 aatatgcaaa agtaggaaat gaaataggta tagcaatcag aaaaaaagtt gttcctgctg
     1621 tgatagttaa gaaacctttt tataaaaaac aatataaaaa agataatatt atattaaaca
     1681 aagaaaataa attttcatat atacctgcta caagtgaaga taaatcaaaa atgttaaaag
     1741 tagttggatt aaattcggtt gatgaattat tttcagatat accagaagaa gtaaaattaa
     1801 aaagagattt aaatttggaa attggaaaat ctgaattaga ggtaagcaaa atagtaaaaa
     1861 gattgtcaga agaaaactta agtttagaag acttgacttg ttttcttggt gctggtgcat
     1921 atgaccatta tataccatca attataaaac atataacttc aagatctgaa ttttatacag
     1981 catacactcc atatcaagct gaaataagtc agggaacttt acaagtagtt tttgaatttc
     2041 aatctatgat tgcagaaatt acaggtatgg aaattgcaaa tgcatcaatg tatgatggag
     2101 caactgctgc aatagaagca tgtataatgg caatgaatca gactagaaaa agtaaaatag
     2161 tagtgtcaaa aactattcat catgaaactt tgtcagtatt aagaacttac ttacaatata
     2221 aagattgtga gatagtagaa attgactttt gtaatgaata tgggactaca gatatagaaa
     2281 aattaaaagc ttctgtagac aaggatacag catgtgttct tatacaaact cctaatttct
     2341 ttggaattat tgaagaaatg gaagaaatag aaaagataac tcatgaaaat aaggctatgt
     2401 taattatgag tgtagaccca atatcattag gagttttaaa aacaccaggt gagataggtg
     2461 cagatattgt tgttggagaa gctcaatcac ttggaaaccc tcttaatttt ggaggtcctt
     2521 atgtaggatt tttagctagc aaatctaaat atactagaaa aatgccagga agaatagttg
     2581 ggcaaagttt agatgtagaa ggtaaaattg catatgtatt aactttacaa actagagaac
     2641 agcatgtaag aagagaaaaa gcaacttcta atatatgttc aaatcaagct ttaaatgctc
     2701 ttgtagcttc aatatatatg gctactatgg gtaaagaagg atttaaagag gttggtatgc
     2761 agtctatgaa aaaagcacat tatacttaca ataagcttgt acaaactgga aaatataagc
     2821 caatatttaa aggtaagttt ttcaaagaat ttgctgtaca aggaaatctt aatatagaaa
     2881 ctataaatga taagctctta gaagaaaata tattaggtgg ttataattta gaatacaatt
     2941 atccagaatt aaaaaattca actttgcttt gtgtgactga gaaaagaagc aaagaggaaa
     3001 tagataagtt agttggaata atggagggat tataatgaaa gaatacaata gtttgttgat
     3061 agatatatct aaaaaaggta ggaaagcata ctcacttcca aagttagata tagatgatat
     3121 aaaaatagaa gatatgattg accaagatat ggcaagacaa agtgagttga atttgccaga
     3181 agttggtgaa ttagaattag ttagacatta tacattatta tcaaataaaa actttggagt
     3241 tgatacaggt ttttatcctt tgggttcttg tactatgaaa tataatccta aaataaatga
     3301 agatatggca gcacttccaa actttactgg aatgcatcca taccaatctt cggatacagc
     3361 tcaaggttct ctttctttga tgtatgattt atcaagaaga cttgcagaaa ttacaggtat
     3421 ggatgaagtt acactacagc cttcagcagg ttctcatggt gagtttacag ggcttatgat
     3481 tataaaagca tatcatgaaa atagaggtga ccataagaga actaaagtga taattccaga
     3541 ttctgcacat ggaacaaatc cagcaagtgc agctatggca aactttgatg tgattcaaat
     3601 agcatctgat aaaaatggag ctgtagacat aaatgcatta aaagaggttt tgaatgatga
     3661 agtagcagct cttatgctta ctaatccaag tactcttggt ttatttgaaa aaaatataaa
     3721 agaaatagct actcttgtac atgaagcagg aggacttttg tactatgatg gtgcaaacat
     3781 gaatgctatt atggggataa caagacctgg agatatgggg tttgatgtgg ttcatcttaa
     3841 tcttcataag actttttcaa ctcctcatgg aggtggaggt ccaggagctg gtcctgttgg
     3901 agttaaaaaa gaattagtac cttttttacc aataccatta attgaaagaa aagaagataa
     3961 gtatgtacta aattatgata gagaaaaatc tattgggaaa attaaaaatt tctatggtaa
     4021 ttttggagtt cttgtaagag cttacacata tatactaact atgggaaggg atggacttaa
     4081 agaggctagt gaaatggcag ttttaaatgc taattatata aaggaaagta taaaagatga
     4141 ttatattttg ccaatagata ccttgtgtaa gcatgagttt gtattaggtg gattgccaag
     4201 aggagaggcc aatataaaaa ctatcgatat tgcaaaaaga ctactagatt atggatacca
     4261 tcctccaaca atgtactttc ctcttattat aaatgaggca cttatgatag aacctacaga
     4321 aagtgagagt atagagacat tagatagttt tatagaagct atgaaaagtg ttgctaaaga
     4381 ggcaaaggaa gaaccagaac tattgaaaac tgctccacat aatactttgg tcaaaagagt
     4441 agatgatgca agggcagtaa agaaacctat actaacatgg tcacaaagat aaataattaa
     4501 aaagtagaga tttaaagtat aaagagaatt aaatttgtaa gaaaaagcta tctaacttaa
     4561 tccagtttag atagcttttt tgtatattag tatgtatggt attttctata taaagtttta
     4621 aat
//
LOCUS       DACTGW010000251         4596 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.254, whole genome shotgun sequence.
ACCESSION   DACTGW010000251 DACTGW010000000
VERSION     DACTGW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.254"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            289..936
                     /locus_tag="I9573_003268"
     CDS             289..936
                     /locus_tag="I9573_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4797858.1"
                     /translation="MNILIADDEISMLKILCAYFKKENFNVFTAKNGEEALDIFYENK
                     IDLAILDWMMPIIDGIEVCKEIKENSNTRVLMLTAKSQSEDEIEALNIGADEYIKKPF
                     DPRVLIIRAKKLINYKNTINIKDLKIDFGSSKVFKGDKELLLTKKELELIRCLINNKG
                     MVLSREKLLDLVWGLDYEGDFRTVDTHIRRLRVKIGEDIIKTYRGLGYSLEDFND"
     gene            929..2323
                     /locus_tag="I9573_003269"
     CDS             929..2323
                     /locus_tag="I9573_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4797859.1"
                     /translation="MTKLNKKLSISISIVVVVVYIISIIINSMFIHRYYLHEKRNILD
                     NVENEIKDKDIDKLKSDITLIEEKNNIDIVYIELDKNHKDKGNIDKINQDLLNAFWKK
                     GLSLNKFWIEENSLNNIDKKSINKIYNQGKTKYSLLVKFIKKDNYLFAISIPIEHSEE
                     TIGIVNRFNIIMGIFSVVIITILTFILSNRVIKPMERLKFLSKDISELNFRTEDIKTN
                     DEIEELAYSINIMSEKLEKAHNELNKRNENLKTFISDASHEMKTPIALIKAYAIGMKD
                     GLDDGTYTDTIIEQAENMTNTINSLLYWAKYEKKERSLCLVDLKERLYKSLKNYELLI
                     ERGSISVKCSVDDKELIISSDEDSIDMVFNNLISNAIKYTNNNEIEINLFEENERIIL
                     SIKNGIDYNMGDDIENMWKPFYVLEKSRSKELSGTGLGLTIVRTILEENNFRYRVEIC
                     DGIIEFYIIFIKVI"
     gene            2748..>4596
                     /locus_tag="I9573_003270"
     CDS             2748..>4596
                     /locus_tag="I9573_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HAT4797860.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAITVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSSKVDKVVLKNTITAANKLYNEAIEGTNVGEYKVGSKAIYKTAIDKAQAI
                     LDKSGVTQKEVNDAVTALNTATDTFKAGKVVAVDKTALQDAVTAATALHA"
BASE COUNT         2001 a          439 c          790 g         1366 t
ORIGIN      
        1 agtatctcag aaagattttt aatcagaatg aggtgctttt tcatttgcat ataaaatatt
       61 aagtaatttc aaaaaaatat aaatttattt aatttaatta aaatattata atatattagt
      121 tacatattaa aatatataat cgagatattg ttaagacatt gtcgagatat tataatatat
      181 aactaatgta ttataaatag ttgtaaactt cctgtaacat atattttata taattaaagt
      241 aatggatata atatgaaata taataatgaa ttttagggat gtgaataaat gaacatatta
      301 attgctgatg atgaaattag tatgcttaag atactttgtg catattttaa aaaagaaaac
      361 ttcaatgtgt ttactgcaaa aaatggggaa gaggctttag atatttttta tgaaaataag
      421 atagacttag ctatattaga ctggatgatg ccaataattg atggaataga ggtttgtaaa
      481 gagataaagg aaaatagtaa tacaagggta cttatgttga ctgccaaaag tcaaagtgaa
      541 gatgaaatag aagcactcaa cattggagca gatgaatata taaaaaaacc atttgatcct
      601 agagttttaa taataagagc taagaaacta ataaattata aaaatacaat taatattaaa
      661 gatttaaaaa tagattttgg gtcaagtaaa gtttttaaag gtgataagga attattattg
      721 actaagaaag aattagaatt aattcgttgt ttaatcaata ataaaggaat ggttttatct
      781 agggaaaaac ttttagattt agtatgggga ttggattatg agggagattt tagaacagta
      841 gatactcata taagaagatt aagggtaaaa ataggagaag atataataaa aacttataga
      901 ggattagggt atagtctgga ggattttaat gactaaacta aacaaaaaat tgtccataag
      961 tatatctata gtagttgtag ttgtttatat aatatctata attataaata gtatgtttat
     1021 tcatagatat tatttacatg aaaaaagaaa tatattggat aatgttgaaa atgaaattaa
     1081 agataaagac atagataaat taaaatcaga cattacttta attgaagaaa aaaataatat
     1141 agatatcgta tatatagaat tagataaaaa tcataaagat aaaggtaata tagataaaat
     1201 aaatcaagat ttattaaatg cattttggaa aaaaggttta agtttaaata agttttggat
     1261 tgaagaaaat agtttaaata atattgataa gaaaagtata aataagatat acaatcaagg
     1321 taaaacaaaa tatagtttgc ttgtaaaatt tatcaagaag gataattatt tgtttgctat
     1381 atctatacct atagaacatt ctgaagaaac tattggcatt gtaaatagat ttaatataat
     1441 aatgggaatt ttttcagtag ttataataac tatacttaca tttatattat caaacagagt
     1501 tataaaacct atggaaaggt taaagttttt atctaaagat atatctgaat taaattttag
     1561 gactgaagat ataaaaacga acgatgaaat agaagagcta gcttacagca taaacataat
     1621 gagcgaaaaa cttgaaaaag cacataatga attgaataaa agaaatgaga atctaaaaac
     1681 ttttatatca gatgcatctc atgaaatgaa aactccaata gcacttataa aagcatatgc
     1741 aataggtatg aaagatggtt tggatgatgg gacttataca gatacaataa ttgaacaagc
     1801 agaaaatatg accaatacaa taaattctct tttatattgg gcaaaatatg agaagaagga
     1861 gagaagtctg tgtctggtag atttaaagga aaggttatat aaaagtttaa aaaactatga
     1921 gctgttgata gaaagaggta gtataagtgt taaatgcagt gtagatgata aagaacttat
     1981 aataagttcg gatgaagata gtatagatat ggtatttaat aatttgatta gtaatgctat
     2041 aaagtatact aataataatg aaatagagat taatcttttt gaagagaatg agagaattat
     2101 attatccata aaaaatggta tagattacaa tatgggagat gatatagaaa atatgtggaa
     2161 gcctttttat gttttagaga agtcgagaag taaggagctt tctggtactg gtcttggtct
     2221 taccatagtt agaactatac ttgaagaaaa taattttaga tacagggttg aaatttgcga
     2281 tggaattata gaattttata ttatctttat aaaagtaatt tgaattaaaa tgtaaatata
     2341 aagaataatt aaaaaaatat cgcaattatt tgtttttcat atggataaaa ttggttattt
     2401 aatatataat tacaagtata aataataaaa tttatttgta aaaagagtag cacttttaat
     2461 tctaaaggct acttttttta tacaaaacca tgttttttat aacaattcat taacataaaa
     2521 atcaaactta ccttaaaata taaatttaca aatttttcaa tcaataagaa aatttctcta
     2581 ttccttacat aatgactgta atgtaacaaa ataattacaa attaattatg aaatcattat
     2641 agttagaaac ctctttaaaa ttagatatta agtatccttt agaattagaa cgggaacaaa
     2701 gttaaaaaat taagtaatat caaatggaca agggggaaga aaataaaatg aaaataccaa
     2761 agaagattgc tgcaatgctt acagttacaa tgatagctgg aagttcaaca gcaggcatag
     2821 ctagtgcaca aactgtggca acaaatttaa cagggcaaga aagatatgaa actgctgtta
     2881 aaataagtca agatggatgg aaaaatgctg atgaggtagt aatcgtaaac gactcatcta
     2941 tagcagatgc tctatctgca acaccatttg caaaagcaaa aaatgctcca atacttttaa
     3001 caagtaaaga taaattaaat gataagacga aagcagaaat acaaagatta aaagctaaaa
     3061 aagtatactt aataggtgga acttctgtat taagtacaaa tatagaaaaa gaaataaaag
     3121 atttaaaaat atcttttgag agaatctctg gagctgagag atatcaaaca tccttagaat
     3181 tagcaaagaa attagatgct ataagtgatg ttaagaaaat agctgtagtt aatggtgaaa
     3241 aaggacttgc agatgctgta agtgtaggag caccagctgc acaaaacaat atgccagtaa
     3301 tacttgctga ttctaaaaat ggaacagcag ttgcagacaa atttataaaa gatgctggaa
     3361 taactcaatc ttatgtagta ggtggagaga gttcaatttc tgaagctgtt aaaaataaac
     3421 taccaaatag tacaagacta ggtggaacag atagaaatga tacaaatgca aaagtaataa
     3481 aagaatttta taaaaagaca gatttaaaaa atgcttatgt aactaaagat ggtatgaata
     3541 aacaagacca acttatagat gcactagcag taggtgtatt aggtgctaaa aatcaatcac
     3601 cagtagtttt agttggaaag aacttatcag cttcacaaaa atctctagtt aactctaaga
     3661 gttttgataa gataactaaa gttggtggga atggtaatga gacagcattt aatgaaatga
     3721 agtcattaca agaagtaaaa acagttgaag ctaaaacaat ttctgaatta aaatcagcta
     3781 tagataaagc tactgctaat gatgttataa actttaagcc aactagtgag gttaaagaag
     3841 catttacaat acaaactgat aaggcgataa cagtaaactt aaatggtaca tatacaaaga
     3901 ctgtgactat caacatgcct aatggtgatg taaataacta tgcaaaagtg gatgatgtag
     3961 ttatagatga tgtaaaagat ggaacttttg ttaactacgg aaaaataact aacttaaagg
     4021 ttaatgataa aaatggagcc aagatagaaa ataattctaa aggtgagatt ggtagtttaa
     4081 cagtagcatc tggagcaagt caagtaaaag tgactaatgg tggtaagata actacagtta
     4141 caaataattc taaaggaact actatagata ataaaggtac aataagtagt gttaagggag
     4201 ataattctcc aacaataagt ggaaatagtc caagttctaa ctcttcagga ggaagttcat
     4261 caagtggagg ttcttcacat ggaggaggaa gttcttcatc aaaagtagat aaagtagttt
     4321 tgaaaaatac aataacagca gcaaataaat tgtataatga agcaatagag ggaacaaatg
     4381 taggtgaata taaagtaggg tcaaaagcaa tatataaaac agcaatagac aaagcacagg
     4441 caatattaga caaatctggt gtaacacaaa aagaagtaaa tgatgcagta acagcattaa
     4501 acacagcaac agacacattt aaagcaggaa aagtagtagc agtagataaa acagcattac
     4561 aagatgcagt aacagcagca acagcattac atgcta
//
LOCUS       DACTGW010000252         4577 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.255, whole genome shotgun sequence.
ACCESSION   DACTGW010000252 DACTGW010000000
VERSION     DACTGW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.255"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(441..2828)
                     /gene="cadA"
                     /locus_tag="I9573_003271"
     CDS             complement(441..2828)
                     /gene="cadA"
                     /locus_tag="I9573_003271"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAT4797861.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLIVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFKEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELVPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGESSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKFNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="I9573_003272"
     CDS             complement(2851..3216)
                     /locus_tag="I9573_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HAT4797862.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="I9573_003273"
     CDS             3415..4263
                     /locus_tag="I9573_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797863.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMSNNMNSNMGMPNNMPNNMNSNM
                     GMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
BASE COUNT         1546 a          801 c          504 g         1726 t
ORIGIN      
        1 cttaaaaata tttcgtcaat acttttttaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg acagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattataac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatatagac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacac aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaatgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct attgctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agatataact atataacctc tatatacttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atagactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaagaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctagaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctagc
     1801 atagaaactc catccactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatccc ctacatttac ttcttctggt gacactacct taacttcaga attaaatttt
     1921 agatttgcat aatctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gatgactccc ctattgttaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcgcc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaactaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagtcctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt taaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa accttgttat taatctcctc tgcacaatgt
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agatgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggttctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaagctt tcctttatat ttactataag ttttttattt
     2701 ataaaattta agttggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacaat gtgcacagtt taatcctcct agtataaatt cttttttaat taaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtttctctaa tatgatttaa
     2881 tccacaatca aatatctggc ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttagtact ctaagttgat gagatattgc
     3001 agagtgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatc tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcatcttt aatcctccat tgttttaata
     3241 tatgaataac tgttcatatg ttcattatat gctatcattc ctaaatttgc aacaataatt
     3301 tttattaatt taatataatt agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaacatg ggtatgccta acaatatgtc taacaatatg
     3841 aatagtaata tgggtatgcc taacaatatg cctaataaca tgaatagtaa catgggtatg
     3901 cctaacaata tgcctaataa tatgaatagt aacatgggta tgcctaacaa tatgcctaat
     3961 aatatgaata gtaacatggg tatgcctaac aatgtgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaataatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctatac aaggtatacc acaggctact aatattgaag aatttgatga ggaagaaatg
     4261 taatatatat ttttttgagc aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgagtatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaaactctt tcttgtgttg gtgggtcaga
     4501 atagtatggt actcgtattt gagactaata ttggcaaaca acataaagaa aagtataaat
     4561 tttttataat aaactgt
//
LOCUS       DACTGW010000253         4535 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.256, whole genome shotgun sequence.
ACCESSION   DACTGW010000253 DACTGW010000000
VERSION     DACTGW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.256"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            332..1633
                     /locus_tag="I9573_003274"
     CDS             332..1633
                     /locus_tag="I9573_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4797864.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1709..1984
                     /locus_tag="I9573_003275"
     CDS             1709..1984
                     /locus_tag="I9573_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT4797865.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3309..3539
                     /locus_tag="I9573_003276"
     CDS             3309..3539
                     /locus_tag="I9573_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAT4797866.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3532..4113
                     /locus_tag="I9573_003277"
     CDS             3532..4113
                     /locus_tag="I9573_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAT4797867.1"
                     /translation="MNNKKHWYDYLWIFSSIYLVLGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
BASE COUNT         1767 a          368 c          891 g         1509 t
ORIGIN      
        1 aagtaagata ttactatcag gtagtgaatt atattgaacg atatatcaat aattggcagt
       61 aagttatatc aaataagata ttactatcga gtaatgaatt atattgaaca atgtattaat
      121 aattggtagt aagttatatc aaataagata ttgctattat gtagtaaatt atatgtatca
      181 gaataaatag tatattaata atttgtagta tatcatgacg aataatatag tcataacaaa
      241 tagtgagtca taacaaataa tgtatttata attgataatg tatttaaaaa tcaaaatata
      301 aaaacttata aggaaagaga gattaattta tatgggtaaa tcaaaagatg ttgaaataga
      361 gaagtctatt ttaggcacaa ttcttttaga caataaatta agctataagc tagatgaatt
      421 aaatgaaaac atgtttatga atgatatatg tcttgagata tttaaaatta tgaaagaact
      481 taaaaaagaa aacatagtga ttgatgtggc cactgtgaaa tcaaagatag atagaaaatc
      541 tctagcaata aaaacgagtg atgtgactaa tctaattact tggggtcaga attttggtct
      601 tgatggtcat attaaaattt taaaagaaaa tttagctaga agaagcataa atcaaaactg
      661 ccaaaacctt ttacacagcc taaaccttgg agaaaatatc gatacttgta tttacaaatt
      721 tgaaagtaat ataaaggaga ttttggacaa ggatacttat gaaaatgatg atgtaaatag
      781 tatagcaggg aaggtgcttg attttttaga aaacaaaaaa gatattggat ttaaatttgg
      841 tataaaatta cttgatacta ctataggagg tctttttaaa ggagagctaa ccacaatagc
      901 agctaaatcg ggggttggga aaactgcttt ggcacttcaa ataatgttaa attcatttaa
      961 gcaaggtaaa aaaactttat ttataagtag agaaatgacc agtgagcaag tatttatgag
     1021 aaatatatgc agagtaactg gtgtaagtac aaggaatatg aaatctaaag aaatagatga
     1081 aaatgattgg aaacttattg taaatgctat aggtgattta agcgaaaata acttaattta
     1141 tattaatgat aagatagata caattagtgc aattagaaag aggataaggc aggtaaagcc
     1201 agatttatta atagtggatt atgtgcaact ccttacatct cagaaaagca tggataagag
     1261 agaaagggag gtagctactt tttcgagaga acttaaaaat atgactttag attttaatat
     1321 acctgtaatc caattaagtc agttaaatga tgaaatgaaa gattcaagac cttatggaga
     1381 tagacctatg agggatagca aagccatcta ccacgatagc aacaatgtgg tttatataca
     1441 tcaattaaag gggagtgatt atgaagaagc agtgagagat attggagaaa gtgaggaggc
     1501 agttagagca tcagaataca ggggaattaa gatggttgat ttaatagttg cgaagtgtag
     1561 agatggacag actaggcata agcatttttg ttactttggg gataagttac attttcaaga
     1621 actaaattat taggtcaata aagtagtaat aacaagctta ttaaaagtca ggaaatagca
     1681 aatagattat aaaatatgag gtgaaaatat gttggaatta acgaaaatta tagaattact
     1741 tgaagaacac aagatagaag taagtattcc aaaggagtta cataagtata gtttattgga
     1801 ataccttgaa ttgtgtcaac aaagtggggg aacatatttt tatattaaaa cactggaagg
     1861 ggcattaaaa gaggctagaa atgagcttat gattaaattg tttaatggga aaaattatat
     1921 ggaattagcg aggatgtttg aaatgagtgt tattaatgtc aggcatattg ttagaaaaaa
     1981 atagatattt ttggagaata caagtgtgat atacttctga aatagaagtt attatagttg
     2041 aaataaaatt gtggctaatt tgtgtataag tttaagatta tagaggagta taagtgtgat
     2101 taacagcatg attttgtgaa taaaattgat gatatatatg agttaaatgt gtgattaata
     2161 aggtaaggtt gtgtataaga ttatgagtat ttaaaaggag gggctataat taatagtgta
     2221 gagttgtatg tttgcttaag attatataat aatttaatag attaaactat acgaataagg
     2281 taaaatcgtg tgtttagtta aagcttatta taaaatcttg atatgcgatt aatgatgtaa
     2341 tatgtgaata aagttaagat tataatagat ttaacctgtg gctaatagaa taaaattgtg
     2401 aataaagtta agattataat aggtttaacc tgtgattaat agagtaagag tgtaaataga
     2461 gataaacttc aatgtgggtt taacttgtgg ttaatagagt aaggtgtaac taaagataaa
     2521 cttcaatatg ggtttaactt gtgattaata gagtaaggtg caactaaaga taaacttcaa
     2581 tatgagctta acctatgatt gatagagtaa gaatgtaaat agagataaac ttcaatatgg
     2641 gcttaacttg tggttaatag agtaagagtg taaatagaga taaacttcaa tatgggttta
     2701 acttgtggtt aatagagtaa gaatgtaaat agagataaac ttcaatatga gcttaacttg
     2761 tgattaatag agtaagagtg taaatagaga taaacttcaa tatgagttta acttgtggtt
     2821 aatagagtaa gagtgtaaat agagataaac ttcaatatga gcttaacttg tgattaatag
     2881 agtaagagtg taaatagaga taaacttcaa tatgggctta acttgtgatt aatagagtaa
     2941 gagtgtaact aaagataaac ttcaatatga gtttaacttg tggctaatag aataagattg
     3001 tgaataaagt tatagtttta gatgggtatt aacctgttac taatagaata tgattaggtg
     3061 taaaattata gagctgtata agaaaatatt atagatatat taaatattag ataataaata
     3121 tttatataga tacacaaaag aaaaggcgat atgaagctaa acttaatgac ttcatatcgt
     3181 tttttatttg aaactccttt gtatttagag tgtgattttt aatattgagg ttttatgtga
     3241 ttaagtcaca gataaaaagt aagtatcttg atataattct tatagattaa taatataggg
     3301 aggttattat gtctaataaa aaaaagaagt atgcaaatat tttagcaaat gagtgcgttg
     3361 cttgtggtag ctgtataaaa gcatgtccaa gaagtgctat ctcagttcca tgtggcatta
     3421 gtgcaaaaat taatagagat ttatgtgttg gatgtgggat ttgtgaaaag atatgtccag
     3481 catctgtaat tgagattata acaatattaa aagaggaaga aggtaagtgt catgaataat
     3541 aaaaaacatt ggtatgacta tttatggatt ttttcttcaa tatatttggt attgggattt
     3601 gtaaatatta ttttcgcttg gattgggcta atttgtttct ttgttccact agctatttct
     3661 attgttaagg gaaataaagc ttactgtaat aagtattgtg gaagagggca actgtttaat
     3721 ttgcttggaa ataaattgaa gctgtcaaga aatagagata ttcctaaatt tattagaagt
     3781 aagtggttta gatatgggtt tttgacattt tttatggtaa tgtttgccaa tatgcttttt
     3841 tcaacttatt tagtgtttga aggaagtaga aattttaaag aagtagtgac tttgttgtgg
     3901 actataaaaa tgccttggca atgggcgtat catggaactt tagtttcacc ttgggtggta
     3961 cagtttgcat ttggttttta tagtgttatg cttacatcaa ctgttcttgg tttgattact
     4021 atgatattgt ttaaacctcg ttcatggtgt gtttattgcc ctatgggtac tatgactcag
     4081 atgatatgta aggcaaagag taagtctgta taggaagaga ttttgtgaag atttaagggg
     4141 tttaatgttt aaagctagag ttgatttaag aaaataagaa agatataaat atctaaatat
     4201 gaaatagatt caagatgagt aatagtttct attagctatg ataggtatat gagtggagca
     4261 taaatgaagt ttgtataaag taaactcatt tatgcttctt ttttacatga tacattacta
     4321 attaaataga aaaacaatgt aacaacttgt attttaagta cgtcagagaa atctgaagaa
     4381 atgataaatg ttatagaaga ttgtaaactt atgcttaaaa caagtaatag atatttaaag
     4441 gaagcagatt aaatttcaag aagtcttacg tgcttgttga aacataaact ggatttgtta
     4501 cagataaact aatttataca atatttttta attaa
//
LOCUS       DACTGW010000254         4524 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.257, whole genome shotgun sequence.
ACCESSION   DACTGW010000254 DACTGW010000000
VERSION     DACTGW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.257"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..351
                     /locus_tag="I9573_003278"
     CDS             <1..351
                     /locus_tag="I9573_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT4797868.1"
                     /translation="NISKLNFIENNDICSIFANSLDNAIEACMDINNELEKRIEVKAT
                     YINKFAIIKFINTKVNDIKLIDKRIQTSKDDDKIHGIGLASIKYIVNKYGGETIVNYS
                     DNEFILKIMIPIKS"
     gene            363..503
                     /locus_tag="I9573_003279"
     CDS             363..503
                     /locus_tag="I9573_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HAT4797869.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            562..1143
                     /locus_tag="I9573_003280"
     CDS             562..1143
                     /locus_tag="I9573_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HAT4797870.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNISVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNNDQ"
     gene            1220..2998
                     /locus_tag="I9573_003281"
     CDS             1220..2998
                     /locus_tag="I9573_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797871.1"
                     /translation="MNLLNIVESIVSCIKGLARRGINMSSIKVISDFITKIIRKNILF
                     AITVIIINYHLIQRWTHISNRGISFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTV
                     KILCRNNFDYFLGISVAVTRNNFIFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILVFE
                     WFLPLTILSIVISFISVIINNLILKITLSSVFLYLTSTKMLDRVIDIKEPFFKALFKS
                     LNIFEDQSYAYYSDFVGKVNNSAYVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLI
                     VIVVYSILFIGLNYISIDMSTYRDEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNI
                     KDRIVLLNTSNEELTNINMILDEIFDIKYIKVNSENIKFNKKKDLIEVNLKESLEPND
                     EVTLDIEYEGYINVLTSWNQNKYIATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSK
                     NNVYSNLPLLNKTSNFLKKDYIFEGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMD
                     IEEKIKFLIETRNEILGDSKASVDAKKYVDEVDNVMESIKRKKINMILVADLPSNEFL
                     YEDSKGEKISITIYHWLSGNILIIDK"
     gene            3013..3885
                     /locus_tag="I9573_003282"
     CDS             3013..3885
                     /locus_tag="I9573_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT4797872.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYIPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINNKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            3886..>4524
                     /locus_tag="I9573_003283"
     CDS             3886..>4524
                     /locus_tag="I9573_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797873.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYLGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQ"
BASE COUNT         1871 a          397 c          667 g         1589 t
ORIGIN      
        1 aatatatcta aattaaactt tattgaaaat aatgatatat gttcaatatt tgcaaattca
       61 ctagataatg caattgaagc atgtatggat ataaataatg agctagaaaa aagaatagaa
      121 gttaaagcta catatataaa taaatttgca ataataaaat ttataaatac taaggtaaat
      181 gatattaaac ttattgataa acgtattcaa actagtaagg atgatgataa gatacatgga
      241 ataggacttg ctagtattaa atatatagta aataaatatg gtggtgaaac aatagttaat
      301 tactcagata atgagtttat attaaaaata atgataccaa taaaaagtta ggagagtaca
      361 aaatgaaaaa aatagcgtta aacttactta aaaacatctc agcattaagt tttgggatag
      421 ctgtattatc agcaaacagt gcaagttctt gggtagcaca tcaggcaaaa gagccacaag
      481 ctttacaaaa actaaaaaaa taacattaat aaaaggacct cttcttatta aaggagaggt
      541 tttacatgtt gaggaggaaa tatgtttaaa agattatcat ataaatttgc caatatatta
      601 gttaataatg agattgtaga aagtgaagat tttgaaatat atagatatgg atttgaaact
      661 ttgatttatt tcatagttaa tatttcggta gcattattta taggaattat ttttgataga
      721 tttatacata cagttatatt tttaagttgt tattgcactt taagacagtt cacaggaggt
      781 tatcatgcta ggaattataa agagtgtaca ttaacatttg cagttattta tttgataact
      841 attttttcag ctaataatat agatataaat aaatataagt atttattagt attattaatg
      901 attattagta tactaacaat ttacaagtta gctcctttag aacatagaaa caaacctctt
      961 agtgaaagtg agaaaaagca ttatagaaag actgtgcaaa aaatactatt cgtaataata
     1021 tgtttaatta ttttatgtaa aatacttaat atttttcagc aatatgtgat ttatgcaata
     1081 atttctatat attggattgc tatattaata tatattggaa tgaaggttaa taatgatcag
     1141 taagattata aaacattttc taaattaatt aatttcacta attaatatta attttaaaaa
     1201 ataatgtcaa ggtaaatatt tgaacttatt aaatatagtt gaatctattg taagttgtat
     1261 caaaggattg gctaggaggg gcataaatat gagtagtatt aaggttatat cagactttat
     1321 aaccaaaata attagaaaaa atattttatt tgcaataaca gttattatta taaattacca
     1381 tctcatacaa cgatggacac atatatccaa tagaggaatt tcatttaatg attacttatc
     1441 cttgagtgta tatacttgta tgacaattag tattttaacc atgttaaatg taatttttta
     1501 tatgagaaaa agttatgata ctgtaaaaat attatgtaga aataattttg attatttttt
     1561 aggtatttct gttgctgtaa ctagaaacaa ctttatattt tgttgcatac caattatata
     1621 tgctatagca tactcaagtt taataagaac attatcaatt gtaacgatta aatcaactat
     1681 atttatattg gtatttgaat ggtttttacc acttactatt ttatctatag tgatatcatt
     1741 tatttcagtt attataaata atttaatact aaagattacc ttatcatcag tatttttata
     1801 tttaacatca actaagatgt tggatagagt tattgatatt aaagaaccat tttttaaagc
     1861 attatttaaa tcacttaata tatttgaaga tcaatcatat gcttactatt ctgattttgt
     1921 agggaaagtt aataattcag catatgttat agataagatt atacctacac tatttgcaat
     1981 tactttaata atagtagcat atctgatatt aaatgatatt agaaagaaac agattaaatc
     2041 attaatagtt atagtagtgt acagtatttt atttattgga ttaaattata tatcaataga
     2101 tatgtcaaca tatagagatg aggaacgtag ttatggagag tatttaaaga aatctaatat
     2161 tagtgatatt aaaataaaat cgcatgatat gaagattgac tttaaaagca agtcaaatat
     2221 aaaagataga attgttttat taaacacatc aaatgaagag ttaacaaata ttaatatgat
     2281 tttagatgaa atatttgata taaaatatat aaaagtaaat tcggaaaata taaaatttaa
     2341 caaaaaaaag gatttaattg aagttaattt aaaggaaagt ttagagccta atgacgaggt
     2401 gacattagat atagagtatg aaggctatat taatgtgttg acatcttgga atcaaaataa
     2461 atatatagct actaaagatg atattatgct tccaccaggt tctttagcgt ggtacccaaa
     2521 agtaaatcaa tcaagttgta tggcttataa aataaattta agttctaaaa ataatgtcta
     2581 ttcaaattta cctttactga ataagactag taatttttta aaaaaagatt atatatttga
     2641 aggaaagtca aatgatattg caatatatgc aggtgagttt aaagaagagg taaaagatga
     2701 tataaaaata atctatccat cagatagtaa tgtaaatatg gatatagagg aaaaaattaa
     2761 atttttaatt gaaacaagaa atgaaatatt aggtgatagc aaagcatctg tagatgctaa
     2821 aaaatatgta gatgaagtag ataatgttat ggaatcaata aaaagaaaaa aaattaatat
     2881 gatattagtt gctgacttac ctagcaatga atttttatat gaagactcaa agggagagaa
     2941 aattagcata actatttatc attggctttc aggaaatatc cttataatag ataaataata
     3001 aaggaggtta atatgataaa caagttagaa ataagaaatc taaattttaa ttacaaaaat
     3061 aaaaaagctc tggataatat aaatttaacc ctaacagatg gagtagttgc attattaggt
     3121 cctaatggtg ctggaaaatc aacattaatg aggttattag tgactcttta tgaaacttca
     3181 attggagaga tagaattaaa taatataaag tatagcaaaa acaatgagaa aatcaaggca
     3241 aatgtaggat atatacctca agattttgat atgtataata atataaatgg gcaagaatac
     3301 ttagagtttg tagctaaaat gagaggtgta tctaagaatg atttaaagaa acatatacaa
     3361 aaagtagtat caaaagttaa tctggacaag tttataaata ataaaatagg tacatactca
     3421 ggtggtgtaa agagacgact aggtatagca caggctttaa taggtgattc taagttaata
     3481 gttatggatg aacctacagt tggtcttgat ccagagcaaa ggaatgaatt tagaagattg
     3541 ttaccagaaa taagtaaaaa ttctatagta ttaatttcaa ctcacatagt agaagacata
     3601 caatttaact gtgacaaatt aattatatta aatcaaggca aaatattata tgatggtaca
     3661 attaataaat ttattgatat ggttgaggtg tattcagtag tagtttctaa tgatgaattt
     3721 gaatatttag aacgtaatat tgatattatt gattatagaa gagttaaaga tggtgtgcaa
     3781 gtaaagtata taggtaaaaa tacagagaaa cttaaggata gcttaaggat agatacaact
     3841 ttacaagatg cttatataaa ctttttacat aaggagttaa aataaatgtg tatattggag
     3901 ttaataaaat ttgagcttaa aaaaatagag cttaaacatg tgttaatatt tgtaattatt
     3961 tctatattat gcatatttat tacaggcata ttagattata atgagttaat attttataat
     4021 gatgtaccta taatggatac atattctata gcaggatggt taggaattat aaagatatca
     4081 gatgttttta taccaataat gtcagcaatc acaataattt atgtgttttc aaaagattat
     4141 ttgggtaata taaatgagat acttacttta tataataaaa aaaaatataa tcaatttata
     4201 gtatttagat ggtgtgttat attagctata tatactgtat tattattaat ttcaattaca
     4261 atagtatata catttacgtc agtgaaaggt tttaacatag acaaaataga ttttgtttat
     4321 agtgtaaagc caatagatgt gtttttaaag acatttccta cattattatg gtatacaaca
     4381 tttccactaa tgatattaac attaacaaaa aatagattta caacaatagc aatattaatt
     4441 acatatacat ttgctgatat attttttatt ttacatatgt acccatttgt tagtatgatt
     4501 aatgtaaatg gattttatat tcaa
//
LOCUS       DACTGW010000255         4516 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.258, whole genome shotgun sequence.
ACCESSION   DACTGW010000255 DACTGW010000000
VERSION     DACTGW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.258"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            235..1386
                     /locus_tag="I9573_003284"
     CDS             235..1386
                     /locus_tag="I9573_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAT4797874.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1435..2607
                     /gene="thiI"
                     /locus_tag="I9573_003285"
     CDS             1435..2607
                     /gene="thiI"
                     /locus_tag="I9573_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAT4797875.1"
                     /translation="MYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVYK
                     EYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEAG
                     YKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFHT
                     MVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTSE
                     RSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQRL
                     SEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIGT
                     FETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2763..3566
                     /locus_tag="I9573_003286"
     CDS             2763..3566
                     /locus_tag="I9573_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAT4797876.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGIEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3809..4450
                     /locus_tag="I9573_003287"
     CDS             3809..4450
                     /locus_tag="I9573_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAT4797877.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1802 a          438 c          879 g         1397 t
ORIGIN      
        1 taatatatta ttttatcata tattgtattt tataatatat ttttgtaagg tatagtataa
       61 gtatattata ttttactata aaaatttgta ataaagtgaa aaaactagct tttcatgaaa
      121 taatatttta taatttaaga ttgtcttttt aataaagatt aacaaaaaaa atactataat
      181 tagtggtata atagttagag ttgaagattt taaataaagg taggtgtaat taagttggaa
      241 atatacttag acaatagcgc aactacaaaa ccttatcaag aagttataga taaaatggtt
      301 tatgcactta atacggaata tggaaatcct tcttctgtcc atagaaaagg tgtagaagtt
      361 gaaaaagcta taaaagaagt aagacaagat atagctaaat ctctaggagc gaaagaaaag
      421 gagatttatt ttacatcagg tggtacagag tgtaataata ctataataag aggtattact
      481 agtttaaata aaaaaagaaa aaatcacata atatctacta acatagaaca tccttctgtt
      541 ttaaatacat taaaagattt agaagaagat ggttttgaag taacttattt agaggtcgga
      601 aaagatggga aaataaatat agaggattta aaaaatgcga taaagtcaac tacatgctta
      661 gtaagcatga tgcatgttaa caatgaaata ggaactatac agccaatagg tgaagttgga
      721 aagtatttga agggtttaaa agaaaaaatt tatttccatg ttgatgctat tcagtcatat
      781 ggaaaaatta attttagacc atctaaatac aatatagatt ttatgagtgt aagtgcacat
      841 aaatttcatg gtccaaaggg aattggattt atgtatataa aagaaaataa tcgccttaag
      901 cctatgttaa caggtggtgg tcaggaaatt ggaattaggt cgggaacaga aaatgtacct
      961 ggaatatatg gtcttggaga agctgttaga atactaaaca aagatttaga tgttgttatt
     1021 tcgaaagtaa ataacttaaa aaatatatta aaaaatgaaa taattgataa tatagaagat
     1081 ataaaaatca attctccaga agatggagta tgtcatatat taaatgtatc atttagaggg
     1141 acaaaaggag aagttttact tcattaccta gagcaaaaag gtatttatgt atcaactggc
     1201 tctgcatgtt cttctaaaaa gaaaggtagt tacgtattaa atgctatagg tcttacaaat
     1261 gaagaaataa atggaacaat aagatttagt ttatctgata tgaatacaga agaagaaatg
     1321 ttagaagctg tgaaagtttt gaaagaatca atttgtgact taaggtcaat aatgaaaaga
     1381 aaataaagaa tactgtatag atagtgataa taaattagtt taagaggtga aatggtgtat
     1441 aatatattaa tagtaaagta tggagaaatt ggagtaaaag gaaagaatag atatatattt
     1501 gagaataggc ttataagaaa tataagaaat atgttaaaac caattggaag atttaatgta
     1561 tataaagagt atggaagaat atatgttgat ttagaagact atgattatga agaagtaatt
     1621 gaagaagtta gaaaagtatt tgggatagtg ggtgtgtgcc ctggagtaag agctaaaaaa
     1681 gattatgata cattaaaaga aatagcttta aaaatgttag aagaaaaaat agaggctgga
     1741 tataaaactt ttaaggtaga gtcaagaaga ggagataagt ctttcgggtt gacttctcaa
     1801 gaaatgagta tggatatagg tggatactta ttatcaaagg taggagatag gattaatgtt
     1861 gatgtaagaa atccagaagt taaaataaag tgcgaataca gagaatttca tactatggtc
     1921 tatagtgata ctatacctgg atatggtgga ttgccacttg gaacgaatgg tagagctatg
     1981 tctcttttat caggaggtat agatagtcca gtagctacat ggatggttgc aaaaagaggt
     2041 atggaggtag aggcagttca cttccatagt tatccattta caagtgaaag gtcacaggaa
     2101 aaagttaagg atttagctaa aatactggca aaatactgtg gtagagtaag acttcataaa
     2161 gttaacatat tagaaataca aaaagcaatt ggtgagaatt gtaatgaaga agaagctact
     2221 attctttcaa gaagatttat gatgaggata gcacagagac tttctgaaaa aagacattgt
     2281 gatgctttaa ttacaggtga aagtattggg caggttgcat ctcaaactat acaagggctt
     2341 acttgtacta atgccgttgt agacttacca gtgtttagac cacttatagc aatggataaa
     2401 tcagatatag ttgatatagc caaaaaaata gggacttttg aaacatctat agtgcctgaa
     2461 gaagattgtt gtagtgtatt ttctcctaga aagcctgtca ctaaacctag attagagaaa
     2521 atagaaaaat ctgaaacagc acttgatatt gaaaaattag tacaggatgc tattgacaaa
     2581 atagaggtag aggatattga gttttagcat acaagttatt taattactaa attgaatatt
     2641 gagtatatta gtagttctgt aagtatatta gtagttctgt tgaaaggatt tatgtagaca
     2701 atttattaat atatagtatt taaaaataag tactataatt ttatttaatg ggaatgatta
     2761 ttatgaataa aaagggtttt acattaattg aattgttggt agttatatct ataataggaa
     2821 ttttagttat agtagctgtt ccagcgttat ttagaaatat agaaaaaagt aaggcagtta
     2881 catgtctttc taatagagaa aatataaaga ctcaaattgt tattgcaatg gctgaggaat
     2941 caagtaaaga caagaatgaa gtcataaaag aggtattaga aaacaaagat ggtaagtact
     3001 ttgaaacaga accaaagtgt aagtcaggtg gaatatattc agcaacgttt gatgatggtt
     3061 atgatggaat aactggaata gaaagcattg caaaagtgta tgttacttgt acaaaacatc
     3121 cagatggtat tgaaatggct agggatatac atcaaagtat gaaagatttg attgcatcat
     3181 ttgcacaaga cccttctata ataccaggag cttcaaaggg caatgatgat tttagaaaat
     3241 atttattaga caataaatat aaaaatgggt ggcctacaat tccagatgaa tttaaggcaa
     3301 aatatggatt aagtaaggat acactatata tacaaccata tgcatataat cctactaaat
     3361 ctgatgctac tgtagttgta tttgcaaata ataagactgg aggtaattgg tatacttccc
     3421 tagtttacga ttatgatgaa ggtagatggt ataaaggtaa aaatggtatt tctgttgcag
     3481 gtaggtcatg ggatgttgac acagatagtg ttaagtctgt aaaaacagag attcattcta
     3541 aagagggatg gggtccttta aattaatata tcatgtttta ttaatctggt attgattaaa
     3601 ctatagaaaa gagcagtttc tatagatgac gaataccaga ttttcattta tatgtataat
     3661 taagatatat taaaagtatt tatattttta agtcttcaca tacaagctaa agataattat
     3721 aataatattg taaagaaatt taattataag attaattttc tgaagattta gtatttgtac
     3781 taagaaatac ataaaaaggg gaatagatat gaataataat tttcaagaca accaaacaat
     3841 aaatttaata caaagtcgta gaagtatacg aaaatttaca acagagcaaa ttagtgatga
     3901 gcaagtaaac acattattac actgtgcatt tgcagcacct tcaggatgta ataaacagcc
     3961 ttggcacata actgttgtgc aagaccagaa acttttaaaa gagattagtg atgatacttt
     4021 atctaggatt catgaagtga gtaatgttga gataaataaa aactttaagt tattttatgg
     4081 tgctccaacc gttttattta tttcttatga tgaaagtagt tcatgggctc catatgatat
     4141 aggaatactt acagggaata taacaacagc tgcacaagca ctagggcttg gaagttgtat
     4201 aataggaatg gttagaggtt tatttacacc tgttgaacaa ggggatattg aaggtcttgt
     4261 gagtgtattg gataaagaag atgtaaagga atcagagtca ataaagatga agtttgatac
     4321 aaataaaaaa tacagagaac tacttgatat accagaaggg tattctgtac catttggtat
     4381 tgctgtaggt attccagatg gaaatttacc aaatgctaga gaggtagttt acaaagtttc
     4441 cagagtttag gataaagtat tgcaagaacc aatcagttac ttgaagaatt aaaccagtaa
     4501 tatacaaata aattga
//
LOCUS       DACTGW010000256         4496 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.259, whole genome shotgun sequence.
ACCESSION   DACTGW010000256 DACTGW010000000
VERSION     DACTGW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.259"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(10..1194)
                     /locus_tag="I9573_003288"
     CDS             complement(10..1194)
                     /locus_tag="I9573_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAT4797878.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1567..3918
                     /locus_tag="I9573_003289"
     CDS             1567..3918
                     /locus_tag="I9573_003289"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HAT4797879.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERFEVQGNKKMKNFPFLMGQGVWKGSDDLVPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            3940..4479
                     /gene="nrdG"
                     /locus_tag="I9573_003290"
     CDS             3940..4479
                     /gene="nrdG"
                     /locus_tag="I9573_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAT4797880.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         1737 a          532 c          750 g         1477 t
ORIGIN      
        1 tctaaattat tatgccatta ttttatatac aaattctttt aatttgtcta atccagataa
       61 aaaagtattt tcactgcaag cataggatat acgaatatat ttatccatac caaatgctat
      121 gccaggaaca actgcaacat tatattcttc taaaaattga ttacaaaatt ctatagaaaa
      181 actatcttta tattcaaatt tctcagatac tttagaaaga tctataaata tatagaatgc
      241 gccatttgga ttcacatatc ctacattatc aatagagtct agttttgatt taattaaatc
      301 tcttcttgat ttatatgttt taaccatatt gtctatgtca attgagcaat ctttcaaggc
      361 tccataggct atgtattgtg ctgttagact tgggtgagat ataagatgtc cttgtataga
      421 actcattgct ttagcaatcg ttttattaga tgcagtatat cctaatctta atccagtcat
      481 tgcagctgac ttagaaaaac catttatagt tattgttata tctttagctt cttcgcttaa
      541 tgatgcaatt gatgtaaatt caccagtgta gcatattttt tcatatattt catctgctaa
      601 aatatatatt ttattttgta tgcatacatc tactatttct attaattcat cttttgtata
      661 aacactacca gtaggatttg aaggattgtt tattacaaga atttttgttt tatctgttat
      721 agatttttca agttcttctt ttgttaactt aaatccattt tcttttttag tatcaataaa
      781 aactggaaca gcattaacta acttaatcat ttcaggataa ctaacccaat aaggctttgg
      841 taataagact tcatctcctt cgtctgttaa agctagtaaa gtatttgtta ttgagttttt
      901 tgctccactt gatacaacta tttcgtctat tgaatagtta cagttatttt cttcaataag
      961 ttttttacag atttcctctc taagtatttt taatcctgga actaaatcat atttagtgta
     1021 atctttattt aaagaatcaa taccataaga ttttgcgtta ttaggtacat taaaatcggg
     1081 ttctcctata ctaagattta ttactttaac tccattactt tccatttctt taactttaga
     1141 actaatacct attgtgtagg atggtgttat aaagtttagt ctttttgata acataaaatg
     1201 cctcctttta aattattatt tatattatac gacaaattta aagaatttac ataaggaaat
     1261 ttgtagatat atacattaat atatataata gcctacactt tttgcctaaa attagtgtaa
     1321 aaaaaagaga aaaaaatttt aactttcaaa gttgttagaa acgctactgt tcaaaatatc
     1381 cacatagtat ttgcaatgat ttgcgcaagt ttgactatta tgtacaaatt tggtaatctt
     1441 aatatagcaa ttattaagat aatcccttta acttagttga aggggatttt tttagactta
     1501 acatactaca tatagtggta ttgggattaa taaaatacaa tatatagtaa caggaggttt
     1561 ttttaattgg ttgaattcgt aaaaaaaaga gatggaagag taattccatt taatgaagat
     1621 agaataacaa gagctatatt tttagcagca acgaatgttg cagaaagaga aggaatagta
     1681 cctgattata aattatctga gcaattaact caagaagtaa taaagttttt aaatcataaa
     1741 tactcagaat cagttccaag tgttgaagat atacaagatt ctgtagttaa ggttcttata
     1801 gaaactggcc atgcaaaaac tagtgaagaa tatataatat acagaactga aagaagtaga
     1861 ataagaaatt caaaaacaag attgatgaag gcaatagagg aaattacctt tgaagatgca
     1921 gaagatgctg atataaaaag agaaaatgca aatattaatg gtaatacagc aatgggaact
     1981 atgttacaat atggaagcac tgtatctaaa gaattttgta aaacacatat attaaaacca
     2041 gaacattctt ttgctcatga taatggagat atacacatac atgatatgga ttttttaaat
     2101 atgggaacat taacttgttg tcaaatagat gttaaaaaac tattcaatgg tggattttca
     2161 acaggacatg gctttttaag agagccacaa gatataataa gttatggtgc attagctgca
     2221 attgctatac aaagcaatca aaacgatcaa catggaggtc aaagtatacc atttttcgat
     2281 tatggattgg ctgaaggtgt atataagaca tttaagaaat tctacatagg aaatttagct
     2341 aaagcattga agttatttaa aggaattgaa aataatgatg tgataaagaa catagtatat
     2401 aatacagaga aagaaacaaa tcaaaaagtt ggtcttaaga gagatgaatt gtatctaaac
     2461 ttagaaaaag agaaattaat acagactttt gatatagatg atgaacttgt aaataagatg
     2521 caaaactttg catttgaaga atcgtataga gaaactgata aaaaaacata tcaatctatg
     2581 gaagcattta tacacaatct aaatacaatg cactctagag caggtgcaca ggtaccattt
     2641 tcaagtgtaa acttcggaac agatacttct gaagaaggaa gaatggtaac taagaatctt
     2701 ctattatctc aagaaagagg tttaggtaat ggagagacac caatttttcc aattttaata
     2761 tttaaggtca aagaaggcat aaatttaaat cctgaagacc ctaattatga tttatttaaa
     2821 ttatcatgca gagtatcagc taaaagacta tttcctaatt ttagtttttt agatgcgcct
     2881 tttaatgcaa aatattataa aaaaggtgaa ccagatacag aggctacata tatgggttgt
     2941 agaacaagag tacttagtaa tgtgtgtggt tcagaaacag ttagtggtag aggaaacata
     3001 tcttttacta cagtaaattt accaagattg ggtataaaac atggtattat aaataatgaa
     3061 aaggctaatt tagatggttt ctttgaagaa ttagatgaga agataaactt aattatagaa
     3121 caattattgg agcgctttga ggttcaagga aataaaaaga tgaagaattt tccattctta
     3181 atggggcaag gtgtatggaa aggttctgat gatttagtac cagaagatac actaaaagaa
     3241 gtaataaaac aaggaacatt gacaattgga tttataggtt tagcagagtg ccttattgct
     3301 cttataggta aacatcatgg agaaagtaaa gaagctcaag aattaggatt aaaaatagtg
     3361 tcacatatga gacataaaat ggatgaagca acggacaaat ataaattaaa tttctcctta
     3421 atgggtactc cagcagaagg tctttcaggt aggtttacta aaatagataa aaaagtttat
     3481 ggagaaatta aaggtatcac agataaagaa tattatacta attcattcca tgtaccagtt
     3541 tattacaata taagtgcata tgataaaatt gagatagaag ctccatacca tgaattgact
     3601 aatgcaggtc atataacata tgtagagtta gatggagatc catctgataa tttagaggct
     3661 tttgaaactg ttataaaagc tatgaaagat ttgggtatag gatatggaag tataaatcat
     3721 cctgtagata gagacccaat atgcggtttt tcaggtgtaa taacaagtaa tatatgccct
     3781 gtatgtggaa gaaatgaaga tgaaagtgac atcaaattcg aaagaataag aagaataaca
     3841 ggatacttag ttggtacagt tgatagattt aataatgcta aaaaagcaga agtaagagat
     3901 agagtaaaac atagatagtt ttatttgggg gaataagtta tgaaaataag aatgtcatct
     3961 actataagtt atgacagcat agttgatgga ccaggattaa gaatggtaat atggactcaa
     4021 ggatgtattc ataattgtaa agagtgccat aatcctcaga cacatgattt atgtggagga
     4081 ttttatatgg atacagaaga aattataaat aaagtcaaat cattgaaatt acaaaaaggc
     4141 attacgctat caggaggaga accattttta caaccagaac cattagaaga aatagcaaga
     4201 gaagctaaaa taaatgggtt agatgtatgg tcatatactg gatttacgtt tgaacaactg
     4261 ttagataaaa aaaatagagc gtattttaaa aatttaaatt tactaaagca aattgatatt
     4321 ttagtggatg gaaaatttat agctgaaaaa aaagatataa gcttaaagtt tagagggtca
     4381 tcaaatcaaa ggataattga tgttcaaaaa agtttgaaat ataaaaaagt gtttttggtt
     4441 gagcaatata tgaaggatga tttatctata gcagaataaa atataaataa acagga
//
LOCUS       DACTGW010000257         4478 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.260, whole genome shotgun sequence.
ACCESSION   DACTGW010000257 DACTGW010000000
VERSION     DACTGW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.260"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            52..1050
                     /locus_tag="I9573_003291"
     CDS             52..1050
                     /locus_tag="I9573_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HAT4797881.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            1412..1939
                     /locus_tag="I9573_003292"
     CDS             1412..1939
                     /locus_tag="I9573_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HAT4797882.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            1966..2562
                     /locus_tag="I9573_003293"
     CDS             1966..2562
                     /locus_tag="I9573_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HAT4797883.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDTVSISLNAPNKEAYNRVTQPKFGEKSFDYMLGFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(2627..4165)
                     /gene="asnB"
                     /locus_tag="I9573_003294"
     CDS             complement(2627..4165)
                     /gene="asnB"
                     /locus_tag="I9573_003294"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAT4797884.1"
                     /translation="MIKILEYNGHIRNWKVLCEELGIDSSLCRDERERAILVEAYKTW
                     GYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETENGKLLYGSSIRKIMEQ
                     PGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQNKTVCIGRYWKPEFH
                     PDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSSYILAMSDIEKTGSCG
                     YEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYHMEQPTGDASAIVFAI
                     GCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYIGNTNIMNEGEKRKIL
                     KKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIYFNIDKMSTAVGLEVR
                     MPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIAFRNKLGFIVPIRIWM
                     ADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWRKIWMIYTFLVWYEIY
                     FVKC"
BASE COUNT         1562 a          698 c          649 g         1569 t
ORIGIN      
        1 tctaaatgaa aaattaccat aataatttaa taattacagg agggtattga tatgaaaata
       61 ctagtatttg gagcacgcga ttatgaagaa ccagtaataa aaaaatggtc tgaagaacat
      121 aaggatgttc aagtggatat ttatcctgaa aacatgactg aagaaaatgt agttaaagct
      181 aaagggtatg atggtatatc tatacaacaa actaactata tagataatcc ttatatttat
      241 gaaactttaa aagatgctgg ggttaaagtt atagcttcaa gaactgcagg ggttgacatg
      301 atacattttg atttagttaa tgaaaatgga cttatcgtta caaacgttcc ttcttattca
      361 cctaatgcaa tagctgaatt agctgttact caagctatga accttttaag aaagactcct
      421 ctagtaaaga aaaaagtctg tgaaggtgat taccgttgga tagctgaact tcttggaaca
      481 gaagttagat ctattacagt tggtgttata ggtacaggaa aaataggtgc tacttctgca
      541 aaattattca aaggcctagg agctaatgta attgcatttg accaatatcc aaatagtgat
      601 ttaaatgata tattaactta caaagattct ttagaagacc ttctaaaaga agctgacctt
      661 ataacattac atactccttt acttgaagga acaaaacata tgataaataa agatactcta
      721 gctataatga aggatggagc ttacatagta aatactggcc gtggtggttt aattaataca
      781 ggggatttaa tagaagcact agagtcagga aaaattagag ctgctgccct tgatacattt
      841 gaaactgaag gattgttctt aaacaaaaaa atgaatcctg gagaattaac tgacccagaa
      901 ataaataaac ttctttctat ggaacaagtt atattcactc atcaccttgg tttcttcact
      961 agtacagcga ttgaaaatat agtttattct agtttaagta gtgctgtaga agttataaaa
     1021 acaggaactg ctactaatag agtaaattag tattttataa atatattttt tcattaatta
     1081 gctattatct aacaaaatag cctttaattt cccaaatata agagagtcag ctcagtatat
     1141 attgagtaga ctctcttata ttatttttta tattaaactt gtactcctct tatttatacc
     1201 aaattaaatt tatattaact tactctaact actcctactc tatacatttt acctattttt
     1261 acttttctaa ttatatacat ttgaatgggt acaatctgta ttttacaaaa aaaacaccta
     1321 acaaactatg aaaattgtat gattaatatt aatatattta taagaaaaca taacaattat
     1381 gttagctctg gatttttagg gagggagtat attggaaaaa atgaataaag ttacttctaa
     1441 aaaaactacc tatattgtta cttcagcatt atttgcatcc ataatatgcc tgacaatagc
     1501 atatatatta catattccag ttggtggaaa caatggatat gttcatatag gagatgcttt
     1561 tatttatcta gctgcaacta ttttgccaac aaactatgct atagctgctt ctgcaattgg
     1621 tgctggtcta gctgatttat ctacaggtgc tgctatctgg gtaataccaa ctataatcat
     1681 aaaaccaata cttgtattat ttttcacttc taaaagtgac aagataataa ataagagaaa
     1741 tatagttgct tcggttgtag caggtatagt aggattagtt ttatacatgt ttgctgaagg
     1801 tataattatt ggaagtttta caagtgcatt tgtaatgagc ttacttggct tattacagcc
     1861 aataggaagt tttattgttt ttataatctt aggtatggca cttgataaac ttgattttaa
     1921 aaaacgttat tttaattaag aatttaaatt tatggaggga aaattatgaa tatcttatat
     1981 acaattgaaa atagtatcta tgtaaatata actaatacat gtccatgtag ttgtgttttt
     2041 tgtataagaa acgaaaaaga tgaagttgca aatagtggta gtttatggtt ggagcatgag
     2101 ccaagtgtag atgaagtaaa agaagccttc aataaataca atttagacga ctatgatgaa
     2161 atcgtatttt gtggatatgg cgaaccatta atgagaataa atgaattgat agaggtagca
     2221 aagtttataa aagaaaaaag tagcataaaa ataagaataa atacaaatgg tctaagtgat
     2281 ttaatacata acaaaaaaac agctattctg ttaaaagatg ttatagatac agtatcaata
     2341 agtcttaatg ccccaaataa agaagcttat aatagagtta ctcaaccaaa gtttggagaa
     2401 aaatcatttg actatatgtt aggttttgca agagattgca aaaaatatat aaaagaagta
     2461 gctttttcag tagtagatga aatatctcca gaagaaatag aagaatctaa acaactggca
     2521 aaaaaacttg atataccatt aagagttaga cataagaatt agcaagtaaa attataaaac
     2581 attaagaatc acctgaatgg tctttcaggt gattcttcct cttgtattaa cattttacaa
     2641 aatagatttc ataccacact aggaatgtgt aaatcatcca aatctttctc caattatcag
     2701 agtttccact aatgtagtca ttaaaaatct cattaatctc atctatgtta aagaatttat
     2761 ctgctatctc actattgaat ttatctcgga tatccttatt atattggtca tcagccatcc
     2821 atatacgaat tggcacaata aatcctaatt tatttctaaa agcaatctct tctggtaatg
     2881 tttttgatgc agctgtacga aaggcaattt tattctcttt tcctttcact ttatattctg
     2941 atggtattct ggatgcaata tcaaatattc ttgtatctgt aagaggcatt ctgacttcca
     3001 atccaactgc agtactcatt ttatctatat taaaataaat atctccttcc aaccatattt
     3061 gaatatctac atttgacatg ttagtaagtg tatctagttc ttctgtttct tcataaattc
     3121 cttttaccac ttcaatagga agcacatcag gattgtattt cttaagaatt tttcttttct
     3181 caccctcatt cataatgttt gtatttccaa tataaaatgt cttcaaattc tctccataac
     3241 actctgcctt atggtatata ttatatccac caaaaaactc atctgctcct tctccagagt
     3301 aacagatatt tgtgttttca gctgctgcct tgcatccaat agcaaaaacg atagcagatg
     3361 catctccagt tggttgctcc atatggtaca ttacataagg tactgacgca aaatactctt
     3421 caggtgtaat tatatatctc gaatttttac acccaagaat attagctgtc tgttgtgcta
     3481 attttgattc atcaaaacgc tcttcctcat aaccacatga acctgttttt tctatatctg
     3541 acattgccaa tatataagat gaatcaacac ctcctgataa gaataactct gcagtttcat
     3601 tctctgattt tacctctggc ataattttct gaagtgttgt atgaatttca tccgtccatt
     3661 cctcaataga cttgctgtgg tcaggatgaa attcaggttt ccaataacga ccaatacata
     3721 ctgttttatt ctgatagaca aggtaatgtc ctggcataag cttctttact ccacggaaga
     3781 atgtattttc acctgctaca tatgtgaaag acatataaat ctgtaacatg tcttcattta
     3841 actctttcac aaatcctggt tgctccataa tcttacgaat agatgaacca tagagaagct
     3901 tcccattttc agtctcataa taatagaaag gttttactcc aaactggtct cttaggcaga
     3961 agagtttctt ttccacagta tcccataatg caaaggcaaa cattccatgc atgtgatttg
     4021 ccatatcata tccccatgtc ttatatgctt ccactaaaat tgctcgttca cgctcatctc
     4081 tacataatga agaatcgatt ccaagttctt cacagagaac tttccagttt cgaatatgtc
     4141 cattgtattc tagaattttt atcatatatg tgtcttttcg tgttaaatac ttttcttcta
     4201 aatttatcat atgtgtatcc tttcgtatta aaacatttaa cttctaaatt tattaaatgt
     4261 ttatatatat ttattcactt ataatcactg aaaaatcgac ttttttcaaa gtaaattctt
     4321 tttgaattag tactaatctg ctctttatta atggcataaa tatgattatc aagtattatc
     4381 agtgttataa cattgaattt tttattcttc ataaatattg ccccctttat attaccaact
     4441 aaatataata tatcttatgt tacttacata ttctttaa
//
LOCUS       DACTGW010000258         4476 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.261, whole genome shotgun sequence.
ACCESSION   DACTGW010000258 DACTGW010000000
VERSION     DACTGW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.261"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..642)
                     /locus_tag="I9573_003295"
     CDS             complement(<1..642)
                     /locus_tag="I9573_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797885.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIV"
     gene            complement(611..1624)
                     /locus_tag="I9573_003296"
     CDS             complement(611..1624)
                     /locus_tag="I9573_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT4797886.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(1646..2416)
                     /locus_tag="I9573_003297"
     CDS             complement(1646..2416)
                     /locus_tag="I9573_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797887.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(2391..2681)
                     /locus_tag="I9573_003298"
     CDS             complement(2391..2681)
                     /locus_tag="I9573_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HAT4797888.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(2812..2911)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      3531..3620
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3964..4320)
                     /locus_tag="I9573_003299"
     CDS             complement(3964..4320)
                     /locus_tag="I9573_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4797889.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKDVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
BASE COUNT         1500 a          746 c          514 g         1716 t
ORIGIN      
        1 tactattgat ggtttttttg aaataacata aattctgttt gatagcatta tagcttcgtc
       61 aatatcatga gttataagaa gtattgttga atttactttt ttcttaacat ctaaaagcca
      121 tttttgcatg gatgttttag ttaatgaatc aagtgcccca aaaggctcat ctaaaagcat
      181 tatgtcatta gaatttataa aagttctcaa gaagttagct ctctgtctca ttcctccaga
      241 aagttctgaa ggatatttat cttcatatcc gcttaatcca aacacctcaa aataaggttt
      301 taccattgac ctagctttac tctttttttc tttttttaaa attagtggta gtgacacatt
      361 atctattata gttttatatg gcaagagtaa atctttttga tacatataac tatatttacc
      421 atttatattc actttgccac tatcataatc tgtaagttta gttataatat taaaaatagt
      481 actttttcca catccacttg gccctaatat agaaacaaac tcaccttcat aaacatcaat
      541 acttatatct tttaaaactt caatatcttt ataagttttt gatacatttt ctatagatac
      601 ttttactttt ttattgtggt aaaaattcat ttgtgtaagc atcacttgcc ttcatatcct
      661 tatctattaa tttatattct tttaagaatg atgtatagtt gttccataca ctatctttca
      721 tttctcccca tcgtggtgca tcatctttgt acttgcttgc taaatattct tgactcttta
      781 aagctagttt ttcatctact tcaggtgcat gttttactaa aatttttgct gattcttctg
      841 gattttttat tgcatattca taaccttttg ttgtagcttt taagaatttt ttagcaagct
      901 ctggattatc tttaagcaca gtttcattag aaattataag tggtgtataa taatccaatc
      961 tctcatctaa atcttttact ggtataaagt ttaagtcata tccaattaaa tcagctttaa
     1021 ctgcatccca accttcaaat atccatgcaa aatctacagt tttcatagct gcaaagaaat
     1081 catcttgacc agtattaact atctttaact tattaaaatc agctccattt ttcttcataa
     1141 ctgctttaaa aacagcttct tctgatggag agccccaacc accataagtt tttccttcaa
     1201 aatctttagc tgttgttata ttcttttctt ttggtgatgc aaacccagaa gtattatgtt
     1261 gtataacagt tgctacagct tttattggaa gtggatcttc actagtcttt gcataagtaa
     1321 cttgttcttg atagcttata ccaaagtcag cttttcctgt tgctactaaa gtttctgctc
     1381 cactttcagg tggttgtact atttcaacat ccaaaccttc ttctttatag taacccttat
     1441 caagtgctac aaataatcca gtatgatttg tatttggtgt ccaatcaagc accatagtta
     1501 cttttttaga agaagtatct ttatcattgt ttttgtcttt attgttattt gaagaacatc
     1561 cagttattgt tgccaatgta aaaacactta ctaaacctaa tattcctaac tttttaaaat
     1621 tcataattgc ctccctcaaa tacctttaat caattaatct atgtcttata acaacttttt
     1681 ttacagcctg tacaattcct acaaacatta aactaaatat aactactaat atagtagatg
     1741 caaatacttt atcaagtgca tatgcacttt ttgacctaag catataaaca ccaagtcctt
     1801 tcgttccacc tagccattct gcaacagttg ctgccattac agcatacgtt gcagatattt
     1861 taagtccaga aaaaaattta tccattgcca taggaaattt aaggtgaatg aatgtattaa
     1921 ctttatttga gtccatagtc ttaaataaat ttaaataatc tttatcaata ttctcaatgc
     1981 catctacaaa acttattaat ataggaaaaa aacaagtaag cattaccatc aatacttttg
     2041 gcattgttcc aaatccaaac catatcataa aaagtggtgc tataattatt gtaggtatca
     2101 tctgtgtaac caacataata ggataaagac atttttttat tattgataca aaatccatca
     2161 atattccaat taccaatgat attataattg cgactataaa accaagtatt gcttctccta
     2221 tagtaaccac agcatgcatg gataaatttt gataatcttt aaaaaacaca ttaattatat
     2281 ctactggtgt gggtagtata tactgaggaa cttctaaaac tcctaccata gattgccaaa
     2341 gaataagtat aaacaaaaat gttgctattg gatatatact atctttgact ttatctatga
     2401 tttctaagtt tttcatctat agtcagacct ccagcttttt catctactct agctactgtt
     2461 atgactcttt cacatccaag attaaaaggg attttatgta ccttttttaa taattcaaaa
     2521 acttcatcaa aatcaccttc aacagtagtg ctcattgctc ctatttcaca tttaagacca
     2581 cttttttcaa tttcagcaat acaagcatct actactttat acatttcttc ttcacctttg
     2641 taaggtctta gtggcattac tgctatgtct gcgattacca tttaaatcac tcctttaaaa
     2701 ataattaata cctactaggt atattttgtt ttttattaaa aaattttatt actaaaaaga
     2761 tattatattt aaataaaaga aaagtcttat ccaaaatagg ataagactta tttttatctt
     2821 aactggtctc cctacactgg cattatccaa acaggttcaa agggtcgaga taaaatctcc
     2881 tctcagcaca ttagctcccc gaaatatatt atatcttttc tacttgatta tactacactt
     2941 atcatatttt aacaaatttt ttatttatat tagatatttt atccgttcaa aaatatctaa
     3001 tatacattgt acattattac accaaataaa ttatcttaaa atggtaattt atcaacatct
     3061 tccaaaacta gattaactga attttctagt ttttattttg ctatttcaag catatttgga
     3121 tgtatatcaa ttccatacaa atattgttct aaatttctgt tttcactaat ctaatttgca
     3181 atcgttacag attacagatt agaaatagct tttagactct acttatcttt atctacaaaa
     3241 aatccttcaa taaattcagt aataatattc agtaatctaa aaataaacct ctatctggat
     3301 ttttaagata caagcctttt aggaagtatc ttttgtgata atataaaaat tactcgtcat
     3361 tatcatcaaa atcctcagca accaacattt tagataagaa tcaaactcta taatataatt
     3421 attttatcca atttattttt gagaaataaa atattttttt aatttttaaa ttttttaata
     3481 tgcaccttga aaaaagaaac gttttcctat attatggtaa tagtagaata aaactaaata
     3541 ggcaaaacta gagaaattta gtgacgcaaa gctataggga ctaagactta tgaaatgagt
     3601 tatgtcagcc agttgccaaa gagatatttt ctttttgttt ttatgaatgt tttttagggg
     3661 gatatatgat atatgtttat ggataaaatg gatagatgta cacatatact aactgcatat
     3721 atatgcagtt cacgcgatta ctgcaatttt atagatacac aattaaatga ttttatacta
     3781 gaatatggag agaatgtggt agaatcttgt ttgcatcaag tgatggtatt ggtaagcaga
     3841 tataattaaa gaaattttat tttctagaaa acccttttta gttttatcta cgttttataa
     3901 ttcattaaag aactaatcaa aataagcttc attatatatt tttcatgaag taaacagttg
     3961 agtttatctc atatactgtt taccccaatt acataattct aataatatag attccaatga
     4021 tttaccacgt ttagtagaca aatattcaac ttttggagga atagtcggat aagaaattct
     4081 cttaataagt ccatctcttt caagatactt taattcttgt gacagtatct tatgacttat
     4141 atgaacaaca tcttttttta attgactgta acgtattggc tcttgtttta aaatatgcca
     4201 taaaataatt gccctccatt tacttcctat cacagtcatg gtaaaaccaa ttgaacaatt
     4261 aaaatctttt tgtctttctt caatatttgg aaattcttca aaattattat ctatatccat
     4321 ctcaatcctc tctttcaaaa tagtaagtat ctaactaaaa agtgcattct tgtttagcgt
     4381 actaattcgt aatataatct tgattaaaaa atagctacgc acactcgtga ctttagtcat
     4441 gagttagtag ctaaaatagt caacatatag ggaaac
//
LOCUS       DACTGW010000259         4440 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.262, whole genome shotgun sequence.
ACCESSION   DACTGW010000259 DACTGW010000000
VERSION     DACTGW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.262"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            152..1627
                     /locus_tag="I9573_003300"
     CDS             152..1627
                     /locus_tag="I9573_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAT4797890.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1714..2127
                     /gene="speD"
                     /locus_tag="I9573_003301"
     CDS             1714..2127
                     /gene="speD"
                     /locus_tag="I9573_003301"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAT4797891.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2157..3008
                     /gene="speE"
                     /locus_tag="I9573_003302"
     CDS             2157..3008
                     /gene="speE"
                     /locus_tag="I9573_003302"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAT4797892.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            2998..3876
                     /gene="speB"
                     /locus_tag="I9573_003303"
     CDS             2998..3876
                     /gene="speB"
                     /locus_tag="I9573_003303"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAT4797893.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4056..4256)
                     /locus_tag="I9573_003304"
     CDS             complement(4056..4256)
                     /locus_tag="I9573_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAT4797894.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1755 a          499 c          761 g         1425 t
ORIGIN      
        1 tttgagtaac ttcaaaaaaa cacttgtaaa aatgctggaa tgttaatata ataaatatca
       61 tgagtttaat aaaagtaaaa ataaaaaata gaaataataa ataataaata ttaaaaatat
      121 ttgaatatag agaggagatg aatttgaaat catggatagc gagtttgcaa atgcagttga
      181 attaagctat gagtgtgctc cattattaga gtcgttaaag gaatactcag agaaagatat
      241 agcttgtttt gatgttcctg gacatgtaaa aaatagaggt gtagctatat taaataaata
      301 tctaggtgaa agtctcatga aaatggatat aaattcatca ccaactatgg ataatgtatc
      361 tgcgccaaat gggattataa aaaatgcaca agatttgcta gcacaagcat atatggctga
      421 tgaggcattc tttataacga atggcacaac tcaagcaata catgcaatga ttttgagtgt
      481 tataaaacca ggtgaaaaag tattacttcc aagaaatata cataaatcag taataaatgc
      541 gttaatactt tgtggaggaa taccaatatt tatacaacca gaatttgatg aaaaactagg
      601 tataagttta aatattactc ttgaaaaagt tagaacagag atagaaaaag attgtaatat
      661 aaaagcatta tttttcctta atccaactta ctatggagtt tgtgcagacc ttgagagtat
      721 tatagaacta tgccataaaa ataatgtttt agtgttagtt gatgaagctc atggtgcaca
      781 ttttccattt catttagatt tacctccttc tgcaataagt ttaggagctg acatggtagc
      841 tgtaagtata cataaaactg gaggagcatt gacacaatct tcagcactct tattgaatag
      901 agataatgta agttttgaaa aggttcttca atcaatcaat atgctacaat caacatcagc
      961 atcttatttg cttatggcaa gtatagatgg tgctagagtt aatctagttg agaatggtga
     1021 aaagcaactg tcaaaagcat taaatctatc taggtatgca aaatctaaat tgaacaaaat
     1081 tgatggaata aaagttttat ctacagaaat attgaaacaa aaaggtgtta aatttataga
     1141 tgaaacaaaa ttgtgtataa atgtaaaaga attaaatttg actggatttg aagtatacga
     1201 tttattatat aaaaattttt caattcaagt agaattggga gattcatata atatactggc
     1261 acttgtgtcc attggaacaa ataaatcaga tatagataga ttagtaaaag cactttctat
     1321 aattgcaaag atttatagaa aagaatcaac attaaatgaa tttaatatgg ttcaaataaa
     1381 tccaattatt aaacttaatc caagagaagc tttttatgca ccaaaggaaa gtgtagaaat
     1441 caattcatgt atagatagaa tttgtggaga atcaataatg gcatatccac caggtatacc
     1501 gattattgct ccaggagaat tgataacaga agaaattatg gagtatatta ttttcttaaa
     1561 aaatagcaat gcgtatctta cagatgtaca agataaaaac ttagatagaa tactagtaat
     1621 aaaataaatt actttatttt gcaagggcaa gtgtttgtta agtagagttt atcaaataga
     1681 acttattaaa agtataaagg agttggggtt gaaatgaaat ttgaaacact aggaagacat
     1741 atactagtag aatactataa ttgtgatgaa aacgtactta aagacccacg tcttatagaa
     1801 aattttatga atgattcagc attaaatgca aaagcgacaa ttgtggattc tgtattccac
     1861 cattttaatc cttggggggt ttcaggagct gttataattg ctgaatctca tttgacaata
     1921 catacatggc ctgaatatgg ttatgcagca gcagattttt ttacttgtgg tgatatagac
     1981 ccatggaaaa gttttgaatt attagagcaa cttttaaagt ctgaaagaag cgagtctaca
     2041 gaaattccaa gaggcttaac tacaaaaatt aaaaaatatt caaaaaaaga tttaggtaag
     2101 attactcata aaccagaagc agtataaaac atacaagaga aagtgaggat tattatatgg
     2161 aactttggta tacagaagaa tggacagaaa atgtaagatt ttctataaaa gtaaataaac
     2221 atctatttga aggaaagtct caatttcaaa ggatagatgt atttgatagt gatgaatttg
     2281 gaaaatttct aacaatagat ggattaatga tggtaacata taaagatgaa tttatatacc
     2341 atgagatgat aactcatgtt cctatggcta caaatttaaa cataaagaaa gtattagtca
     2401 taggtggagg tgatggaggt acagtaagag aattaagccg ttatcctcaa atagaaaaga
     2461 ttgacatggt tgaaatagat aaaatggttg tagatgtatc taaggagtat atggatatat
     2521 gctcatgtaa attagatgat aaaagagtga gtttatattt tgaagatggt gttaattttg
     2581 ttaagtgtgc acatgataaa tcatatgacc tgataatagt agattcaact gacccaatag
     2641 gaccaggcga agggcttttc tcaactgatt tttataagga ttgttataga atactgactg
     2701 atgatggaat tttagtaaat caaagtgaaa gtccgtattt tgactttaat gctaaagaga
     2761 tgaaaagagc taataaaaaa ttaaaacaga tatttccaat atcagaagtt tatcaagcac
     2821 atataccaac atatccatct ggtcactggt tgtttggatt tgcttcaaaa aaacttaatc
     2881 cagtaaaaaa tcaagataga aatggatggg agaaattaag tttaaaaact aaatattata
     2941 atagtgatat acatttaggt tcgtttatgt tacctcaata cgtgaaggag atgctagatg
     3001 aagaataatt tctatcatat gaacactttt atgagtatgg acaaaaatta tgaagaatct
     3061 aatcttatag tatttggtgt tggatttgat ggaactactt ctaacagacc tggggctaga
     3121 tttgcaagta gctctatgag aaaagaattt tacggtcttg agacatatag tcctttttta
     3181 gatttggact tagaggatta taatatatgt gattatggag atttagaaat tagtgttgga
     3241 agtacagaac aagtcttaaa agaaatctat caagagacat ataagattgt tagagattca
     3301 aaggtaccct ttatgattgg aggagagcat ttagttacat taccagcctt taaagcagta
     3361 catgaaaagt acaatgatat atatgtaatt cattttgatg cccatactga tttgagggaa
     3421 gaatataata atagtaaaaa ttctcatgca acagtaatta aaagaatatg ggatattgta
     3481 ggtgataata aaatatttca atttggtata aggtctggga caaaagaaga atttaaattt
     3541 gctacagaag aaaaacacac atacatggaa ataggaggaa tagatacgtt tgaaaatata
     3601 gttaatatgc taaatggaaa gaatatttat ctaactatag atttagatgt attggatgca
     3661 tctgtgtttc cgggaacagg tacaccagaa cctggtggtg taaattatag agagtttcaa
     3721 gagattttta agattataaa aaactctaat ataaatatag ttggttgtga tattgtagag
     3781 ttaagccctg attacgatac aacaggtgta tcgacagtta tagcttgtaa aatcttaaga
     3841 gagttatgct taataatatc tgataaaatt aaatagagta ttttatataa atagtatata
     3901 ttataatata agaagttatt ttaaaaaagt tagtttaagt ttgacgcttt tctaaagtaa
     3961 cttcttattt ttgtaattaa aaataaatat aataaacaac aaaaaagagc cctcgatagc
     4021 tccttttcta aaatatatat atttaaatta aataattata ttctatttac gttttcagct
     4081 tgaggtcctc tattaccttg agttatatca aagctaacct tttcgccttc ttctaaagtt
     4141 ttgtatccat cattttgtat agctgagaaa tgtacgaaaa catcatcttc tccctctaca
     4201 gatatgaacc caaatccttt ttcattgtta aaccatttta ctattccgtt tttcattata
     4261 agaaaacctc cgcattatta aaaaatacaa actaaaaaaa taagtctgtt ctattaatat
     4321 aacataaatt ttaaataaaa gcaaataaaa tttttaaaaa aattaattac ttttagaatt
     4381 taataaaaat tgttaaatta tacagtgaga agtattgtaa aatttgacga aaatgtaaat
//
LOCUS       DACTGW010000260         4437 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.263, whole genome shotgun sequence.
ACCESSION   DACTGW010000260 DACTGW010000000
VERSION     DACTGW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.263"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            991..2247
                     /locus_tag="I9573_003305"
     CDS             991..2247
                     /locus_tag="I9573_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel protein"
                     /protein_id="HAT4797895.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFLLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGIAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKTLGLEKFS
                     FALSSKVVFDLPIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            2310..3503
                     /locus_tag="I9573_003306"
     CDS             2310..3503
                     /locus_tag="I9573_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAT4797896.1"
                     /translation="MLTSLALIFLLGMASGGIFEKIKLPSLLGMLLTGIILGSYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMNIGIFLQIKYRVVARRLSVKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGIASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     EGKSK"
     gene            3710..4090
                     /locus_tag="I9573_003307"
     CDS             3710..4090
                     /locus_tag="I9573_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT4797897.1"
                     /translation="MYLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKILSKLSKKRFLYVQETGKCMYYTVAIKEKTYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYI"
BASE COUNT         1606 a          447 c          783 g         1601 t
ORIGIN      
        1 attttttata gtaattttaa ggttctacaa tacttcagtt gaaaaatatt taacagcata
       61 gtataatagg aaagaaaata ttttaaataa actttagtat acaaagtgaa gaaaaatatt
      121 taaattatat ataatattgt agagtgaggt aaaatattta agttatatat cataatacag
      181 agtgaagaaa aatatttaga taatgtacca taatacaagg tgagtaaaaa tatttaaagc
      241 atgtataata atgcaaagcg aagaaaaata tttaaataat gtaccataat gcagagtgag
      301 taaaaatatt taaataatgt gttataatac aaagcgaata agattattta aagcatgtat
      361 tataatataa agtgagtaaa aatatttaaa tcatgtatca taatataagc agagtgaaga
      421 aaaatattta aataatatat cataatgtag agtgaggtaa aatatttaag ttatgtatca
      481 taatacaaag taaagaaaat catttaaatt atatatcata aaaataatat gaaatattga
      541 acactcataa tattggaggc tttaatattt taattgatag gttacattat ttataacatt
      601 aaaaatacaa tttacataaa atagacatat gaaaaataaa aaattatata ataattatgg
      661 gaatgaggtt ctcccttgga tgataatatc ctaaactgcc aatgagctga tgacttctat
      721 gatatcatag atgccaccag ctttttttgt tgtttaaatc gataaaatgg aggtgaatta
      781 cttactaaat ttttattttt taaatttgaa gaaatattta attaatagaa aaaacagtag
      841 attatatgtt aattatttta ttaagaatgg gttaagagta tacctatatc ctatcaattt
      901 aaaaaacaat tatgatagtc attacattga ctacagttga tgcaataaaa ctttcaaaaa
      961 atcttatgat gcaaatagaa aggaattaaa atgatagaaa aatttaaaac tttaatatgg
     1021 attaaaatga taataagaat taaaagcgtt tataaaacat atggtggact atttttgtta
     1081 ggactcatag gtataccagt tggtgcaatt ataggtctta tagacaccat atttggaacg
     1141 gttcttctaa aagttacaga tattcgagaa acatatccaa tgtatctgat accattttta
     1201 gcagtagtag gagttgttat tgcttattgc tattttaaat ttggaggaaa aagtagtaaa
     1261 ggaatgaatc taatatttga ggtagggcat ggagaagaag aaattattcc tttaagattg
     1321 gttccattta taatatcagg tacatggtta acccatttat ttggtggtag tgcaggacga
     1381 gaaggagttg ctgttcagat tggtgcaact ttttcacatt gggtaggtaa aaggttgcct
     1441 ataaaaaatg cttctagtat ttttctagta actggtatag cagctggttt tgcaggatta
     1501 tttgagacac caatagcagc aatactattt gctatggagg ttttggtagc tggttcactg
     1561 gagtatcaat cgctttttcc tgcgtttact gcttctttta cagcaagtgc agtatcaaaa
     1621 actttagggt tagagaaatt ttcttttgca ctttcttcta aagttgtatt tgacttacca
     1681 atattttgga agcttattgt tttaggaatt atttttggaa tggttggtgg agcatttgca
     1741 tggtgcctga aattgtcaaa aagaaaaata ggtaatagat taaaaaatcc tatgataagg
     1801 attgctatta ttggagtatg tcttagtgta ttattccttt tattttataa aggaagatat
     1861 tcaggtttag ggacaaattt aattcaaaat agtttttatg gtggagaaat ttattcattt
     1921 gactggctgt taaagtttat acttacaata ctgactttgt cagcaggttt tcaaggagga
     1981 gaggttacac ctttgttttc cattggagct agtcttggtg tattgttagc aggatttttt
     2041 aatttgccaa ttgaattagt tgcagcgctt ggctatgcta gcgtctttgg cagtgctaca
     2101 aatacttttt ttgctcctgt atttataggg gcagaagttt ttggatacag ctatttacct
     2161 tatttctttg tagtatgcgc tatttcatat atatttaata tggataaatc tatttattca
     2221 ctacaaaaaa tttcaacgaa acaataaata aaacaacata atgtatgatt ataagagaaa
     2281 gaatattaga gaaaggatag ggtaggaata tgttgacaag tttagctttg atttttttat
     2341 taggaatggc ctcgggaggt atttttgaga agataaaatt acctagctta ttgggaatgt
     2401 tacttacagg aatcatattg ggttcatatg ttttaaattt gattgataat tcaatactag
     2461 atatttcttc tgaccttaga aagattgcat tgattattat attaacaagg gctggattat
     2521 ctttagatat aaacgatttg aagaaagtgg gaagacctgc tgtacttatg tgctttattc
     2581 cagctacttt tgaaattatc ggaatgatag ttttagcacc aaaattacta ggcgtttcta
     2641 tattggaagc agcagtcatg ggtgcagtag taggagcagt ttctcccgca attattgtac
     2701 ctaaaatgct taaattaatg gaagaaggtt atggtacaga aaaaagtatt ccacagatgc
     2761 ttttagcagg aacttctata gacgatattt tcgtaattgt gatgtttaca gtattcacag
     2821 gattggcaca aggaaatagt atatcagcta ttagtttttt acaaattcca gtatcaatta
     2881 tattaggtgt aatagttgga gcagtgattg gaatctgttt agcagtattt tttaagaagg
     2941 ttcatatgag agattctgct aaaggggtgt tgcttttaag tatctcattt ttaatgataa
     3001 gtttggagac tgctttagag ggtattgtgc cattttctgg attattagca gttatgaata
     3061 ttgggatttt ccttcagata aaatatcgtg tagttgcaag aagattgtct gtaaagtatt
     3121 caaaactatg ggttggtgct gaaattctct tgtttgtttt ggtaggggca acagtagata
     3181 taagttatgc atttaaagca ggaattggag ctgtaatttt aatatttgga gttcttttat
     3241 tccgtatggt aggagtattc ttctgcctta ttaagacaaa tttgacaata aaagaaagat
     3301 tgttctgtat gattggctat attccaaagg ctacagtaca ggcagcaata ggaggtgtac
     3361 cacttgcaat ggggatagca tctggacaac ttattttgac attagcggtt ctcgctatac
     3421 ttataactgc acctcttgga gcttttggaa ttgatgtaac ttataaaaaa ttattaactt
     3481 ctgtagaagg taagagtaag taagaataaa aataaagatt attttaagtg ataaattata
     3541 ttttggaaaa aactatatta ttgagtacaa tgttagaaaa tcatttgagt gattacagaa
     3601 ggtagaagtt tatatcggat tttaaggcat gtgatgatta ttagaattga ttttgtgaaa
     3661 ttgttattgt aatatttaat tatatagata aagaagcgga gtgataacta tgtatttaaa
     3721 aaaattatca aaattagagt tagttattat gaaatttatt tggaatttag atataaaaac
     3781 caattcatat gagattattg attatatgaa agaagagcat aatttaccag aaaaagtggc
     3841 tttaaaaatt ttatcaaaat tatcaaagaa aagattctta tatgttcaag aaacaggcaa
     3901 gtgtatgtat tatacagttg caataaaaga aaagacatat ttagagttta tatcaagaaa
     3961 tgtgcaaaat cttttgaaga ataattttat aaggaattta ttagtatcat ttcacgaaga
     4021 ggagctaaca gaggaaaaga taaagtcgtt agaaaactgg gtagtaaatt gggaagaagc
     4081 ttatatataa atgttattat ttttattaca atttagataa ttttggtttt gtaagctctg
     4141 caactatttt tagatggttg cagagcttat ttttattttt tattccctta agcaatactt
     4201 aaaagtaaac gcatttatat ttactaactt caatcacttc taatacttag attacttttt
     4261 aaaaatattt tgatatgtca tctctatagt aatctagaac ttgtttctat tctttatata
     4321 aagtttttat gaatgatata tataatattc gttcatttga ttaaaaataa caaaatatta
     4381 aataattcta ctctgatagt ttgttaaaaa aataataaaa aatattaata aacaaaa
//
LOCUS       DACTGW010000261         4424 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.264, whole genome shotgun sequence.
ACCESSION   DACTGW010000261 DACTGW010000000
VERSION     DACTGW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.264"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            729..1103
                     /locus_tag="I9573_003308"
     CDS             729..1103
                     /locus_tag="I9573_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4797898.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFVSTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMGILYEED"
     gene            1105..1962
                     /locus_tag="I9573_003309"
     CDS             1105..1962
                     /locus_tag="I9573_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797899.1"
                     /translation="MENILEVYNLNKTYTNFSLSNISFVLPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMSEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            1965..2603
                     /locus_tag="I9573_003310"
     CDS             1965..2603
                     /locus_tag="I9573_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAT4797900.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            3577..4311
                     /locus_tag="I9573_003311"
     CDS             3577..4311
                     /locus_tag="I9573_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT4797901.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMVLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELTPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRDIIGTLKNLIIE"
BASE COUNT         1647 a          487 c          657 g         1633 t
ORIGIN      
        1 attattaaaa ttattttttg ttttatttat caaagagtat tcgtgtttta gaagttagta
       61 ttttttattt tatatatcca aggttttccg tttctaatgt tttctttttt aaaataatta
      121 gggtattcaa ttatagtaaa aggtggatgg tattttggaa tgatattatt taaagcacag
      181 tattgaatgt tgaaatcctc acattttaga gtattatctt ttgtaatcaa aaaaatcttc
      241 tatacttata gcaaggttac ttctgcaaat attccatgca tcataatatc caaatttaaa
      301 tttttttaat atagagttta cctctagtct acactcttga ataactcttt cctctatcca
      361 aattcttata aactcatccc atttattact atctatttat tactatctaa attttttagt
      421 atgtttgtaa ttggaaatgg atttattcct ttatgttttt tataaatagt ttataactta
      481 atttattatt aaacaaatca ttcatacaga attacttttt cttttagata ttcaattctt
      541 acttttaata aatatttaat acattctatc ttatgtggtt ttaaaaagtg tttatggcta
      601 tcaataatct ctaatatttc attcatattt attttgtcat agtaatattt tacatctttt
      661 atattgacaa aaaagtgctc taagtgtata gtgtgtatat aataacatca cactggaggt
      721 gataaaaaat gaaagtgctc atttctaaca catcagataa gccattatac caacaaatta
      781 aagagcaaat taaagaagca atatttactg aagaactgaa agaaggggat gcaatgccct
      841 caataagaaa ttttgctaat gatttaaaag ttagtatttt gactattaga cgagtgtatg
      901 aagaacttga aaatgaaggt tttcttacac gccaagttgg aataggaaca tttgtttcaa
      961 ctggaaattt agaaatacta agagactcaa agaggcgtat agttgaacaa aaaatggcag
     1021 atatgattaa ggatgcaaaa gcactaggaa ttacacagat agagttacaa gaaatgatgg
     1081 gtattctata tgaggaggat taatatggaa aatattttag aagtttataa cttaaataag
     1141 acttatacca atttttcatt atctaatata agctttgtat taccagaaga ttgtataact
     1201 ggatttatag gtgttaatgg agcaggaaaa acaacaacaa ttcgttcgat attggatgtc
     1261 gtaaagcgtg atagtggaaa aataaaaatc tttggtaaag atttcgatgg aaatgagaag
     1321 gaattaaaaa atcgaattgg agttgtattt gatgattcat atttctatga ggagttgtca
     1381 atgtcagaaa tgaaaagtat tattgcacct tcatatgata attggtgtga agaagattat
     1441 caacaaaata tgaagcgttt taatcttaat ccaaaacaaa agattgatac actttcaaaa
     1501 ggaatgaaaa tgaaatttgc tttagcatta gcattatcgc ataaagctga gtttttaatt
     1561 atggatgagc ctacaagtgg tttagaccca ctaatccgta gtcagatact tgacatacta
     1621 acagaatata tgaagaaatg tggtcgtggt gttttctttt ctactcatat aacatctgat
     1681 ttagacaaaa tagcagatat gattgttatg attgacaatg gacgtattat attagagcgt
     1741 gaaaaagata ctctattgga tgaacataga aaagtaaaag gggatattca atttctaaat
     1801 gatgaaatca aaaaattatt tttaaattta gaaattacag gttatggttt tatagggatt
     1861 acctcaaaaa ttaatgaagt gcaaaaaaat atgccagata ttttaataga gcgtcctaca
     1921 attgaagata ttatgcttgc atatattgaa aggagagagt aattatgttg attcatttag
     1981 ttaaaaaaga tattctgatt gtaaaaaaat atgtattgtt gatattgttt attgcaatag
     2041 gaatcccatt atttatatta tggcgtttac ctccatttgc acaaatattg ggatttgtac
     2101 tatcaattat attttcagaa tttatgctgt gccagtatct attattaaag gagagtcaat
     2161 attcaaaagc atctgtatta ctttgttcaa ctccttatcc tagacgtggc ttagttcaat
     2221 ctaagtatgt tttatttgca atggtattta tctattgtac attagtttac tggattgaaa
     2281 atttacttat tccacaaata ggaacttttg acttaacata tattttgatt gttttattaa
     2341 taagttctat tatttatgga gtttatatgc ctattcaata caaattagga tatgaaacaa
     2401 caaaattctt tttcgtgatg ataatcatgg cttcatcatt tgtattacca ttaactatag
     2461 ctcacagcaa ttttgaaatt ccatttatat atcagtatcc atccatgata gtgaatattg
     2521 tattgttcct tttaagttta attatactgt tagcttcaat gtttatttct attaaaattt
     2581 ttagtaataa ggagcttgta taaggtaagg gggaatttta tatgccacat ttgccagatt
     2641 gggctgtttt actttgtatc ttacttccaa tagtatataa agcttgtcag atatttaaaa
     2701 gtaaaaaata gagtacatat ttatattgcc atttttattg agtgaagtta gatggtatga
     2761 atttaaaagg ttacttgaaa ataaagttac ttggtgtggg agagtaacat cataatatta
     2821 tagattatat gtttcttgtg aatcaatttg tattttttaa tagataatat ccagtaattt
     2881 tattttccat tgattatttt caattatgag tatgattgtg tgtattgtat accatctttt
     2941 aaggtgtttt taaaaaatta tatcgtcaaa atataaatct aaggaatcct ataaataaag
     3001 cttttcaatt ttagaaaact aataaaaagt aggaagttaa taaaaagaag ctgtctcaaa
     3061 atagagataa gcttcttttt cattgctgaa atgacgaaaa tataattgat ttaatataaa
     3121 aaagagtgct ttatgaacta tttattttga acagtagata cacttgcact caatttagtt
     3181 actcctaagc tttatgaact aaaagtttat tttgaagcct atttttctaa ataatcatct
     3241 atatacagta ttgcgtttct tttatttatt cataatttat tttcttatta aagtttttct
     3301 atattacttt acttttaatt ttatacgaat tatcaaaaat aaaatagtta ttagaaaaaa
     3361 ataaaagtaa ttgcaaagat tgcaattact tacttctata ttaattacta taccataaaa
     3421 taacaaaaaa atcaagtcga gtaatccttc aacttaagtg atatactagg tattaataat
     3481 ttttaattaa taaaatataa ctagaggaaa atctacattt tctcaatttc tttaattaaa
     3541 ttgacctcta attaagtaat tataaggaga aaaattatga atttagcaaa gggattagat
     3601 gttttaaaaa tttcatcaaa tgtattcggt gaagataaag ttatgtatat tcctgtaatc
     3661 tacacagaag atgatgcaac acttattgac acaggtcttc cgggtcaagg tgatttaata
     3721 attgatgctc ttaataaaag taacacgagc tttgataggt taaaaaatat aataattact
     3781 catcatgata tagaccatat aggaaacata aattatctaa gagaaaaatc taagaataat
     3841 atcaaagtat atgcatacaa aagtgaagtc agttatataa caggagaaga aactcctttt
     3901 aaactttata tgttggaaca aatggtagat aaaattgatg ataagatgct tagtatgcta
     3961 aatgttatgg tacttggatt taaatcatca tatactaaag tagatgtgtc attagataac
     4021 catgaaaaat tgaatctagg tgaggaaata gaagttatac atacaggagg tcacactaga
     4081 ggtcacatat gtctttattt aaaagagtct aaagtactta ttgctggaga tttgttacag
     4141 gtagaaaatg gagaacttac gcctgttgat gtaatgcaca gcaacaaaca agagttaaaa
     4201 gatgctatta aaaatataag taattatgat atagaaacga tagtttttag tcatggtgga
     4261 ttatatcaaa gagacataat tggaacattg aaaaacctaa ttattgagta gtactatatg
     4321 atttacaata atttcttaaa ttacttaaaa ttatatctaa agatgtagaa tcaaaattaa
     4381 atataagtaa taagaatttt gctttaaaat tatacttaag tgtt
//
LOCUS       DACTGW010000262         4279 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.265, whole genome shotgun sequence.
ACCESSION   DACTGW010000262 DACTGW010000000
VERSION     DACTGW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.265"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(231..740)
                     /locus_tag="I9573_003312"
     CDS             complement(231..740)
                     /locus_tag="I9573_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HAT4797902.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(742..1389)
                     /locus_tag="I9573_003313"
     CDS             complement(742..1389)
                     /locus_tag="I9573_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797903.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(1403..2575)
                     /locus_tag="I9573_003314"
     CDS             complement(1403..2575)
                     /locus_tag="I9573_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAT4797904.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAE
                     RLKLEFEKEGLDCELVDVGENGNTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGALCAFNMETGLVDNSLCIGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSRENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            2843..4219
                     /locus_tag="I9573_003315"
     CDS             2843..4219
                     /locus_tag="I9573_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797905.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEILNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
BASE COUNT         1502 a          745 c          478 g         1554 t
ORIGIN      
        1 atagagataa gcctctcttt cattgttgaa attatgaaaa tatacttgat ttaataccaa
       61 aagagtatct catgaactaa aaagttcatt ttgagatact ctcttctttt ttaattaata
      121 tacatattaa ttactatatt agtttattac tatatatcaa ttactattca ttaattacta
      181 ttcatcaatt cttatatatc atttataatt aggttataat tttatgttat ttacattaat
      241 cccataacag tgagtaaaac tcttactatc aacatcccca ctattgcatc aaatatagga
      301 actgccaaca gtaacattct atacttagta ctggcatttg ctacaagcac tattctggca
      361 taatgaccaa ctagagtacc cattaacata catggcataa ttaatatggt tgcttgtggt
      421 gcagtgatta gaccttgtgc aaataagttt gcagctgtag ctgctcctgc tgccttagca
      481 aagaatgctg ctatcaaaac tacaacagat tccccaggta gtccaaaaat acccattact
      541 ggaccaaaaa tatttcctaa aatatcaact aaaccagtta attgtaaaaa ctgcactatt
      601 acataaccca ttatcatggc tggtaatatc tgttcaacac ctatgtaaaa accttttttt
      661 gctcccccca taaatatctc aacagcagtt ttcttaggtt tactatttac aattgcattt
      721 ccagtatttt gttgactcat actacacagc ctccttagcc ttattctttt tatttcttac
      781 acctatgatt aatctaacta gattagcacc aagtatttta cagaatatct ctataaggat
      841 tattggacct aatgccaata atgctattgg taaaagtgga gcttgagtat ttattgtgtt
      901 cagtataaca gctgaaccag catattgata tgctacaaat atagttcttt catcatctgt
      961 tatttcccca ctttcaaata attctttcgt cattattgag gctacatctg aactagtaaa
     1021 tgaagataca aatgctattc ctgctattcc aggtatacca agcagaggtc taagtaatgg
     1081 attaaatatc tttgatgctg ctttcattcc tcccatagat tcaactacat ctataagacc
     1141 tacagcaaaa cagacagttg gaatcaaagt cagagcaaat aagaaaccat cttttgctcc
     1201 cgttcctcct ttacccataa agtctacacc atctgcaata gtaccaaatg aacctgtcag
     1261 attagcaaaa tctaatgctt taaggaagcc ttgactatct ttaaatactc ctgaaaataa
     1321 tactatcaat acaaataaag ataaataccc tttccatgtt accttattat ctccagctgt
     1381 tacatccata taaaatcccc ctttatacct caaatttatc taagtttaaa ataactgtag
     1441 aaattaattt caccctctca aacatagaat ctactactgc atattctctt gatgtgtggt
     1501 tccattctcc tcttacacca aatgaacaga ttgtaggaac gtttgcaata gttaaataac
     1561 ttgcatctga actacctcca agtctcttag catttaattc tccaaatcca ttttctcttg
     1621 acacttcatt tacaaatgta tgaaatctca taacatcttc tgtagtttca aatggcatca
     1681 tttcacttat aaaattaact tctgtagaag ttccttctat gtaagtttct ttacatatct
     1741 ctctaacttg ttctttaaca ttctccattt cttctacttt ttcaaatcta aggtctactt
     1801 ttatactaca atcttctgga atagaatttt ccactcttcc acccttaatc acacttacac
     1861 ttactgtagt tccaacatct aaattagtca atttttgtat gcgtagtatc ttattagcca
     1921 tttcttctat agcatttctt ccaccttcaa aatcattacc tgcatgtgtt tctacacctt
     1981 ttacatgtat attacatcct attcttcctt ttctacctat acataaacta ttatctacta
     2041 atccagtttc catattaaaa gcacaaagag caccttttgc ttccctcaat ataacatctg
     2101 caccagttga atctttatga cctatctctt catctccaga aaatactatt tttattggtc
     2161 tttctttgta tcctatttta tttaaagctt taataacgta taaagagatt attatacccc
     2221 ccttcatatc taaaacacct gggccatatg cttttccttc tattatttta aatggattct
     2281 ctccaaatgt acctgtttca aagacagtat ccatgtgtcc tgaaaaaatt atcggctttt
     2341 tatctatatt agaaccaagt gtacctatca atgtattacc attttcccca acatctacta
     2401 gttcacaatc caaaccttct ttttcaaatt caagctttaa tctctcagct aacttgttta
     2461 cactttcttt acaatgtgta taactttcta tattaacaat ctctttccat aatgaaacta
     2521 tttcttctct attctcatca acaaacttag aaatttcatt taaatatgta ttcataatct
     2581 tctcctttat tttcaaataa aatgtatttc tttcctaaaa tctaagttta ttttaggaaa
     2641 tcttaaaaga ctagtcctta attacaaaat agcacaatta ttcttgccag tcaacatatt
     2701 tttcagtctt ttttatattt tctgattttt ctttcaatta tccttttgta aaataaattg
     2761 acgcgtttta tctactatta taaaatgagg ttataaattg attgattagt tacaatactc
     2821 tcgaaatttt aaaggaggtt ttatgaaata taaaattggt tttttgacaa catcttattt
     2881 tatgtcagat aagtttaaac aagctcttaa agaattaaat gatacttgtg aaattacatt
     2941 tattgagctt aaaggggaga atccttttaa taatttacct aatatatact atgagcaagt
     3001 agataatttt gatggatttg tatgcagtgg tatagttcca tatagtgaac ttataataaa
     3061 tataaaagac ataaaagttc cattaaattt tttaaagctt gatgaaagag atttttataa
     3121 atacttattc aaattattaa atagccaaaa agatattgac tttacaaaat catttatgga
     3181 ttttttaaga gaggacaaca attattatga tatatattca ttaatagact ctaataaatg
     3241 tccatataca attaaagatt ttaatatccc tcaacctgta tatgacttta aaaaattaaa
     3301 tgataaatta ttggaaatac atttagactt attgcgagat aataagatta acctttcttt
     3361 tactagaaat tacatcatta gttgtgaact taataagctt ggatatgatt gtatttacat
     3421 ggttccatct acagaatcaa tcctgaatac atttaaatct ctggtgaatg aaataacatt
     3481 acaaaactta gacaaaaata aatctgcaac ttgtatagtt acagtaaata gttcagagta
     3541 cataaatgaa gatttgatat ttaaaaatga tgagatacaa aatcaaatat ataacactat
     3601 attaaattct ctatcaagac atggattcta tggagttcag gtcaaaaaaa atgatatgaa
     3661 aatagagata cacactacta aagaaatatt aaacaatatg accaataact atacagattt
     3721 ttttatttca gaagatttaa aagaaattaa atatagctta aatataggtt ggggcattgg
     3781 aaatacaaat attcatgcag agcaaaatgc tcgtcaggct aatggtaaat ctgcttcttt
     3841 gaatggaaac tgtactttta tagtaaatga tactaatgat acaataggcc ctttatattc
     3901 aaataaaaat tctaataata ttgaagatag ctcaatagcc aataaagttg cttcttttat
     3961 acctctatct aatacaaatg tatctaaaat catgtgtatg attaatgata gaaattcaaa
     4021 taatgtgtca gcagaaatat tagcagacta tatgaacata actcttagaa gtgctaatag
     4081 aatattgagt atcttatata aagcaggaat tgcaactatt gtaaatacaa aactcgataa
     4141 tcaaagagga aggccaaaaa aaatatataa agttgatttc ttatcttttc taaataagat
     4201 gcagaaatta aattcttaat atttaatttt ttaataaaag caatgtattt tctctaattt
     4261 taaagaatca aaatgttta
//
LOCUS       DACTGW010000263         4272 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.266, whole genome shotgun sequence.
ACCESSION   DACTGW010000263 DACTGW010000000
VERSION     DACTGW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.266"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(126..501)
                     /locus_tag="I9573_003316"
                     /pseudo
     CDS             complement(126..501)
                     /locus_tag="I9573_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            complement(606..983)
                     /locus_tag="I9573_003317"
     CDS             complement(606..983)
                     /locus_tag="I9573_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT4797906.1"
                     /translation="MKEEILIEKLLRAELIVMEYLCKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINFQYHYKIIIDNKEYYTFKKKVLKSSKSIKIMRSLT
                     TTHKNVSKEKLDLLDEYYRNLKE"
     gene            1789..2283
                     /locus_tag="I9573_003318"
     CDS             1789..2283
                     /locus_tag="I9573_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAT4797907.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIIALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKNDYGNLIDITNDQNKINIVIKLLNN
                     KSNT"
     gene            2680..2830
                     /locus_tag="I9573_003319"
                     /pseudo
     CDS             2680..2830
                     /locus_tag="I9573_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3306..3494)
                     /locus_tag="I9573_003320"
     CDS             complement(3306..3494)
                     /locus_tag="I9573_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT4797908.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGEGALKIKKFSILYGSS"
     gene            3656..4006
                     /locus_tag="I9573_003321"
     CDS             3656..4006
                     /locus_tag="I9573_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HAT4797909.1"
                     /translation="MIQKKIFMGFCGFLGFLSLRYFSSGNVTDLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNVELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            4008..4208
                     /locus_tag="I9573_003322"
     CDS             4008..4208
                     /locus_tag="I9573_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012422935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4797910.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
BASE COUNT         1527 a          516 c          480 g         1749 t
ORIGIN      
        1 atataaataa aatacaaact tatttcattt ttttaacatg ttcagaataa taaacaaaat
       61 gaatgtagaa aaagttataa tttcatacct tcattttatt tattatgtat ctagctgtca
      121 ttttcttatt ttaaattctt aaaatattct tctaataaat ttaaaatatc accatctact
      181 tcatcatcct tactacatag tgctgacatt aaacttaata ttgaattttt atgtgtattc
      241 tttaaaaatt cttttgttta aaatctctga tattattttt cttttactaa tacattatag
      301 tgtgtacgct tatctatttt ctccgtttct aaaaactctc ttctttagta atctttttaa
      361 tactgtaaat atagttgtat ctttccattc atatttttgt tccatagact tggtgatttc
      421 tcttgatatt aatacctttt ctgattccca aatatatctc ataactttta attccgcttc
      481 tggtaattta ctaattttca tagtatacat ccctttacca atttatgtat atgttaaatt
      541 ctatatcaat aaagttttac aaacaaaaga attaaggtaa ataattacat tcgattttta
      601 ctatatcatt cttttaaatt tctataatac tcatctaata ggtctaactt ctctttactt
      661 acatttttat gtgtagtggt aagtgaacgc attattttta tgcttttact agattttagt
      721 acctttttct taaaagtata atattcttta ttatctatta ttattttata atgatattga
      781 aaatttataa tatatctagc caaatacctt ttagcaacta gcctttttag tagtattttt
      841 attgtacttt tatgccatcc ataaatctgt tttatttctt ttacaatttc ttttttaggc
      901 attagagtgt cttttttaca taaatactcc attactatta attctgctct taataatttt
      961 tctattaata tttcttcttt caatttatgt cacttcctta attcttctaa cttaaattaa
     1021 taataggagc tttttaagac aaaaactaga ggtaagtttt accaaatatt ctacatattt
     1081 agtctgtttt tttaaacgta aaataatatt catatatata tatttgttta atttgcaaac
     1141 aatgctacca aacaataaat agctctattg tattatttta gttgtagaat agcactaaat
     1201 cgttagaatc aaagaaattt agtagcacaa aactatatag gctaatgctt ataaaataaa
     1261 ttataagtta tgttaacaag ttactaaaag catattttat ttttgttttt atgaaagttt
     1321 tttaggtatg taataatata tatcttttaa aaatatgtat tcatgtatac atattttgac
     1381 ttattatact gataattcat ataattattt tgattttata gatacacagt tagatgattt
     1441 tataatagag cataaaaaaa atgtagtata atcttgttta tatcaagtta tggtattggt
     1501 aagtaagtat aattaaatag acattatttt ctgggtcctc tttttatttt catctgcttt
     1561 taaataatat tattgacatt gatatgactg tagttttctt tattttaatc cattgcttat
     1621 atcttttcta acaagaaatg tcattttaaa tcatttctat aaattgtaaa ataatagtat
     1681 atttgtcata gaaagtgatt tttttgtcta tatatggtaa aatatagaaa gtagaaatca
     1741 acaaaataaa aatttatata ttattaattt tatatggagg ggaaataaat gaaaaataaa
     1801 aaaggattta ctctagtgga attattagta gtaattgcta taataggaat attggcaata
     1861 atagcacttc cagcattatt taaaaatata gaaaaagcaa agatagctaa acttgaagct
     1921 gatataagtg caataaaaag tgcatctctt agttactatg ctgatgaatc caagtatact
     1981 gatggaggaa tgatatcatg ggtaaagaaa gatggaaaaa taataataaa tgggggtttt
     2041 aaagatgacc cattagcaga taaaatagaa aatttaggga tgccttataa tggttcatat
     2101 ctgttaatgt catctcctgg tcatgaaaaa tatctagaat taagcatact tccagaagga
     2161 gaaataagca aaagtggtct agataaatta aaaaatgatt atggaaattt aatagacata
     2221 acgaacgatc aaaataaaat aaatattgta ataaaacttt taaataataa atcgaatact
     2281 taaatacaat aaatttgatt taataattac tgatgaaata aacaatcttg tttggagaaa
     2341 tgaaaaaagc actccccaag aatagaatgc ttagttataa ctacagaagt gtattggcat
     2401 acacttctat tttttattat atcacataat ttatgttgct aaaaatacct atttaagttg
     2461 tttttaaatt gtgttgagta atattcttga tagtttatac attaatgaat ttcaaaaaaa
     2521 taagcactct ttataataaa aagtacttta gtgtatgtta atgtcctatc taataaaatt
     2581 gtaacatgtc ttatgcttta gtatgataat tttcattctc tttattgttg caacttatac
     2641 tatagttttg ctaatttatc tgcaaacaca acttctttat tgcatgtgta tcaaccaaaa
     2701 acatcagaat acttacttaa tacatctgca aaatcgtatc tttcatctat agtattctta
     2761 tttgacaata taacaaaaaa tgaatcgaga tatgcctttt ttataattag atatatttta
     2821 taaattctag catatcattt gttattttct gatatgctat tgatgtttta aatcttctat
     2881 ttattttatt attttttact tttccaattg tacctttata ggagttatat ttatgtgatt
     2941 tatgaaaaaa tgctatgcat ttaagttcta attttttgat aatacatcta actttctttt
     3001 gattaataat gataccacta tttttaatgt ggcttccctg aggcacacat cttttttaaa
     3061 aactcaagct catcttttaa aaatttattt tcatattcta attcttgttc tcttgttaat
     3121 ttattttttc tttagtgttt ttgtttttat tcattatggt cttcctttca ttttgaaaaa
     3181 ggcatcaaca ccactatgta aaaatttttg ttttcatgtt gctataactg tgctattgtt
     3241 tattccaaaa tacttagcga tttattgtac actatactta gttatcatca tatagtttat
     3301 aacatctaac ttgaaccata tagtatagaa aactttttta tttttagtgc accctctcct
     3361 aatatattaa cccatctttc aacttgagaa tggtcataaa ctccatattg ttttactatt
     3421 aaaatatctc ttccttctct attcaaataa aatttagcta cttttaattt gaaattaaaa
     3481 tcatatttag tcatacataa acatctcaag cgttagattt ttactctaac aattatggtg
     3541 cagtacaaat attgtataat actgattttc tattgacaaa agaatataaa agagatatat
     3601 ttgtattata aagatacaaa tatatctctt ttaaaattac taaggaggat aaaaaatgat
     3661 tcaaaaaaaa atttttatgg gtttttgtgg tttccttgga tttttaagtc ttagatattt
     3721 ttcatctggc aatgttactg atcttacata tattggattt tttgcttttt tctcaaactt
     3781 tattatagca aaaataaatg gtgataaagc agatgaaagg tatgtacaag atgaaaaagc
     3841 agctatggca tttacaggac agcttgctat tatagaatta ttcattttat ggtgtattac
     3901 tatagtgtct aggaatgtag aattaatgtg tgttttacta tcaattacat atgcaatcac
     3961 tttaaatgtt tatgctataa aactttatat tttagaggaa aaataatatg cccaaattca
     4021 tttgtaacct aaaaaaatat agacaattaa aagaattaac tcaagaacaa ttagcagaaa
     4081 aagtaggagt tagaagagaa accattatgc gtttagaagc aggaaagtat aatccttctt
     4141 tgaaattagc aatagatata tcaaaaatag tagatacacc tattgatgaa ttgtttattt
     4201 ttgattagtt tttgcattat taaaaatgtg ttttaaatac aaacttaaat tcaatcttta
     4261 agtcactcct ga
//
LOCUS       DACTGW010000264         4165 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.267, whole genome shotgun sequence.
ACCESSION   DACTGW010000264 DACTGW010000000
VERSION     DACTGW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.267"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(57..1073)
                     /locus_tag="I9573_003323"
     CDS             complement(57..1073)
                     /locus_tag="I9573_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAT4797911.1"
                     /translation="MGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKAREF
                     GTLHGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQAITCLKHKKHVLCEKSLASNL
                     KEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYDK
                     YKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSAV
                     FQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIPK
                     EKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVYP
                     ADSI"
     gene            complement(1252..1791)
                     /locus_tag="I9573_003324"
     CDS             complement(1252..1791)
                     /locus_tag="I9573_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HAT4797912.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPILVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(1892..2572)
                     /locus_tag="I9573_003325"
     CDS             complement(1892..2572)
                     /locus_tag="I9573_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAT4797913.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(2601..3890)
                     /locus_tag="I9573_003326"
     CDS             complement(2601..3890)
                     /locus_tag="I9573_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAT4797914.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         1394 a          736 c          380 g         1655 t
ORIGIN      
        1 aaaattaaat ttatttaaga atatatatta taaattctta tattttctaa tataagctat
       61 atactatcag ctggataaac aatactactt tgtcttctta tctcatccat aatttccatt
      121 actattattg aattttcatg agaatttata tttgattcta ttttattgtt atttataaga
      181 cttataaact ctacaagttc ataatacatt ccttctattt ctctaggctt ttcctctgct
      241 ttttcttttg gaatagatat atcttcttct cttccatctc tgtatttaat aatagctttt
      301 tcaaatagat taagcttttc aattattata cttccctctt ctccttgtat ttcagatggt
      361 atataagaat cagctacttt agaatactga attattgcat ccatatcatc atattgaaat
      421 acagctgaac cttctccatc cactccagtt tgaagtatat gagaaactgc ctttacagtt
      481 tttggagcac caaataaatt caccatagga tgtatacaat acactcctat atccattaaa
      541 gctccatttg acaactcttt tttaaaggca ttttctataa ttccattttt atatttatca
      601 tatcttgaag aatactgaca atagcttcca aaaaatcttc ttattttccc tattttatac
      661 aagttttctt taactgcttt aaagtttgga acacatgtaa ttctcatagc ttccataagc
      721 aatacattat tatctttggc tgtttttatc attgatttga cttcttttaa attagatgct
      781 aacgactttt cacaaagcac atgttttttg tgttttaaac aagtaatggc ttgctttgaa
      841 tgtaatgcat ttggagaagc tatatataca gcatctatat ccttcgattt agccatttgt
      901 tctaaatcat caaaaaaaat acttgctcca tgtagagttc caaattctct agctttttct
      961 aaatttctag aatatacagc aaccaattca aaatctttaa ccctagatgc agctcttaga
     1021 aatatatttg ctatattgct tgtcccaaca ataccaaatc ttattttacc catcataaat
     1081 cactccccat tttctacact ttattttaat tttaataaaa aactttacaa cttatagata
     1141 tattataaaa taattttatc aaataaaaaa gaaagctaat tttaaatgat ttcaccattt
     1201 taacatagct ttcttttaac tacaatctaa attcaactta tatttttaaa gttatctttc
     1261 tactatatct ttatttcccc tcaaaaattt tatagtaaga atttcatcca caaacaatgt
     1321 tactaaaagt atagttacaa gaattggcca ataaggtatt ataagactat ataaactatc
     1381 aactatatac acttgaaaat atctaactcc aataaatgtc aatatagtcc aatatacaga
     1441 aaactttaga catactattc ctctactatt gtacttataa tctgaataat cccaatagac
     1501 cttattaaaa tatactttca taagtattcc tgtaatatat tctactactg tcggcactac
     1561 tagacataat aaaagcaatg caaatatatt attggggtca ataaatcttg agacctctat
     1621 tataatcgac attgcaattg catacattgg tttaaatgga ccatttaaaa atccatcttc
     1681 ttgaaaatgc ccttgtttat aataactata taagttttca attatccaac ctataaaacc
     1741 atacaagaaa aaattaaata aaaaatataa tagagaacct acattatcca tgaatattcc
     1801 tcctagctgt ttatacttat tttttctcat attcatggat ttattctaaa tcaaagaaat
     1861 tatatttaat ttatttaatt aatactttat attatagatt gtgacattct ttagagttca
     1921 aactttttag gtattctcta acttccagta aattttcaag tatcttatgt gattgtgatt
     1981 taatttgctc atctggctct tttaaatctg gtacatttat acatcttatc ccaccattgt
     2041 atgcagcttc aactcccata ggcgaatcct ctatgacaat acaattttgg ggattaacat
     2101 ttatcttttt agctgctttt aagaatatct cagggtttgg tttagagttt acaacatcat
     2161 ctccacaaac tatagcatca aagtaatctt ttaaatttgc cttagctaat ctctttactg
     2221 ctctttctct ctttgttgat gttgcaacag ccattttgta tccattttcc tttaaaaaag
     2281 agattagttc attgactcct aatttgatag gagctccctt cctctccata aattcaatca
     2341 tatttttagt tttttcgtca tataaatcta ttattggtac agaactatcg tatatatctg
     2401 taagaccttc tattattcct ttacgatttc ttcccataac tgatgtataa atttccttag
     2461 tcatagtata tccatacttt tcaaacgtct ccatccaaaa ttcaagagaa attctctctg
     2521 agtcaaacaa aactccatcc atgtcaaaaa taattccttc tactttttgc atacaattcc
     2581 tccaatattt tttgatataa ttatattaca ctttttatga ttaattttac aaccctcaat
     2641 ttatccaatt ctaatattgt aaaattgtaa ttttcaaaag ttacgctttg gtttacttta
     2701 atatttgaac caagcatata ggatatccac ccacctatac tgtcaaatcc atcatgctct
     2761 atatcaagtc caaaatactt attaatatag ttgatagatt cagttccatc aactaagtaa
     2821 gaacctgctc ctattttctt aatatgagtc tcttctttgt caaattcatc ttgaatttct
     2881 ccaactatct cttctagtat atcttctatt gttaccacac cagatgtacc accatattca
     2941 tccacaacta tagcaagttg aagtttttcc tttcttattc tctcaagagc tttatctata
     3001 gttaaatttt cagatatata tataatatct ctcaatattt cttctagttc aattttattt
     3061 tcatttatct tttgattgta taaatctctt atatgtacaa aacctaatat atcatcttta
     3121 ttttttctgc atactggata tcttgtaaca ccttcctctt ttaaaatagc taatatcttt
     3181 tcttcagaat cactttcata tatacatacc atatcagttc taggaaccat tatctctcta
     3241 attttctttt cttcaaattc aaaaatatta tcaactaatc tttgctctga ttcatctata
     3301 agtccacttt tataactttc ttctactaat agtcttattt cgtcacctgt atggggttca
     3361 tcaacctcat cagcttgtga atatccaaat ggtttaagaa ataaatcagt acttaagttg
     3421 aaaaaatata ttattggata tgttaactta taaaatccta ctaataaaaa agatgtactc
     3481 aacattattt tttctgtatt ataaagtgct aaagctttag gaaccaactc tcctattaca
     3541 acctccatca ttgttataat taaaaaagaa atggaaattg atatagtata tataatactt
     3601 tcactaagat taaatacact tactattggt aaaattaact ctttaactgt agattctccc
     3661 atccaaccta atgcaagtga acaaagtgtt ataccaagtt gacatgcaga aagataggaa
     3721 tttaagttgt cttttattat tttacatctt tttgcattat tattaccttc agttatcatg
     3781 gtatcaatcc tagaactacg aagttttacc atagcaaatt cacaagctac aaaaaaagca
     3841 tttatcaaaa caagcattac tataagtgtt acttttaata actcccgcat aaagtattgg
     3901 ttcttataat gtaagaaatc tataagaact caaagttccc cccttatatt tataaattta
     3961 atttataata tatattctat catatttatt aatatatcct actttattac aaataatttt
     4021 tataaactaa ataacccctt taatatttat gactgtctat ttgataaata ttaaaggggt
     4081 tatttagtta tcatttttta tttctaaaaa tcttttctca aatgatttag ttatctattt
     4141 atccaatttt ttacataatt taatg
//
LOCUS       DACTGW010000265         4159 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.268, whole genome shotgun sequence.
ACCESSION   DACTGW010000265 DACTGW010000000
VERSION     DACTGW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.268"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(35..1294)
                     /locus_tag="I9573_003327"
     CDS             complement(35..1294)
                     /locus_tag="I9573_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance protein"
                     /protein_id="HAT4797915.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            complement(1533..2162)
                     /locus_tag="I9573_003328"
     CDS             complement(1533..2162)
                     /locus_tag="I9573_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAT4797916.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            complement(2859..4019)
                     /locus_tag="I9573_003329"
     CDS             complement(2859..4019)
                     /locus_tag="I9573_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HAT4797917.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALVVTQILHMLA"
BASE COUNT         1403 a          726 c          424 g         1606 t
ORIGIN      
        1 cttttatatt acaaatattt ctctaaaaaa ttatctattt ttttaattct ttataagaac
       61 tatttgatat atgttctttt tttatgacct ttccacttga atctttaaat attcgatatg
      121 tagaatattt attctgacca gtttgtttta cctctataga tatatttggt ttgtcaccag
      181 aattaccata gattactgat gatacacttc ctccacctgc ataattttta atatatacag
      241 gatgcttata tggattttta aactttaagt ttaatcctcc atcgctcaca gtagcatctc
      301 ttcctttagg cacataagta gatgcagatg aatgatttgt aacttgagtt atctccatac
      361 ccgaaagaag agccgcatta taaactgttg aagatgtttg acaaacacct cctcctgctg
      421 attgttctag cttaccataa actataacag gtgcatcttt gtaaccattt gctttattac
      481 taggtcctgt aagtttattg tatgaaaatt cttcacctgg cattaataat acatcactag
      541 tttttcttgc tgataatcct acattatatg acctaccctc tacagcttgt gaataagtag
      601 ttgaatattg acctataaca gaatttacag tttttacttg ttcagttgtt atatctggtt
      661 tttgaagatt tactttaagt tctaaagttt tatgctcttt attttttatc atattatata
      721 tagactcttt atttgctgcc aaatcaaatt ctctacctac aacagagtct gtataactta
      781 gtccaccact tccaacacta atagaagcat ttttcatctt aacatttata ctatctgcta
      841 ttgaagacac tttggaacta agttttgcct catcatattc agcttttatc ttcatatttg
      901 ccttattcac tcttatatca atatattttt ttaagttttg aaaataggac cctttttttg
      961 tactttcata agcatctttt acaacatctt ctgtatcata ttttaaatca atatcttttg
     1021 gtgatattgt atattttttc ccatcataag taagatttaa gtcttctgga gtatacttat
     1081 ctgttatagc ttttaatgcc tctgctttag tcatatcaga tacatttact ccttctatat
     1141 atacattttt agcgattttc ccattgtaca gaaatttact gttaaaaaaa taagtaaatc
     1201 cacccattat tagaactaaa gctgccaata caattaaaac acttcgtttt ttttctttac
     1261 ctaatcttga aactcttgtt gttcttctgc ccataacttc acctcatttt gacactagct
     1321 taaatttgca taatagagtt cccatttcaa aactacctta gattatattt gaatagcttt
     1381 tgttaaacaa ggaatttaat caaatgtaac tttattgtaa atttatgtct tataattagt
     1441 tttatttttg atatttttta ttatatttct tttactattt cttaacaaaa aattgtttat
     1501 tttataacaa ctaatttcat ttatattgaa atttaaatgt aacctattac aggataatgg
     1561 tctgacaggt aaattttttc tactgtgtat ccttttaatt cagtattttt attaacaaaa
     1621 atgtaatcta ttctagagtt tgatttttca aatgttggta agtaagactt gtttagtgaa
     1681 atagccatat cattaaacat acttaaaaag acatttttct cattaaaatc accacataaa
     1741 ataactatcc ccactaatct gttcctatag tctattattt cgtctaattg ttttgctctc
     1801 tcctgcctat caagtcccaa gtgagtattt attacattta taattcttcc atatgcatct
     1861 atagttatac aaagagctcc ccgttgttct tttttgctag ttaagaaaaa atgattactg
     1921 tttaacattt tagatgttgt aaatgtacaa ataccatata tcatatttac tttttttaca
     1981 tttgtagcaa agacaccatc catatttaaa acagctttta aagctaaaaa ttgaggatat
     2041 aatacttctt gcaaacaaat tacatcacaa tccaattgtt ttaaatataa ccccatttta
     2101 gttagagtta atctattatt tgaatccata cctttatgaa tattgtaggt tactattttc
     2161 ataataatat cacctcttat gcaatataat acttactaaa atttaaactt atttactttt
     2221 cacttaagac cattatttat cttataaaat tgagtactta taaactttat cacaaaaaaa
     2281 cctaacttaa gtatttatat tctacataag ttaggtttta taaactatta taatatttat
     2341 tatttcattc atttctaatg atgattctcc ttttttatct ttttcaattt gccttcacta
     2401 acaacaaata aaaatcccat cttcttgtat atttcatcca attcaattaa agttacttca
     2461 ccatttttta aatttgttaa tgttatatct tgtattcttc ttgatggaat tatcataagt
     2521 tatcaacact cctttaaatt ctttttaatc tttatattta aatatattta tgcattctga
     2581 tatatattca taaattatta aaataaattc aaaaaacacc cgttctgcaa acattcgttc
     2641 tgtatattta ttatacatca tacagacgtt cgattcaata taaaaattaa atatatctcg
     2701 tttttagtaa attttacaat attatacact aaatatgtca aaacatcttc ttgaaataga
     2761 ttatcttgtc aaacaaaaaa aaccatttca gtaaaagctt ctgcttaaat tgaaatgact
     2821 tttcttattt aaattattaa ttttatacca tatttttatt aagctaacat atgtaatatt
     2881 tgagttacca caagagcaca ataagtccct ataatatttc ctagaagccc cataagtaca
     2941 cctactggta ttaatacagg attgtgaacc ccagcaatca ttggagctgt agttactcct
     3001 cctatattag ctatacttcc tacttcacaa gtaaataaat caagtttaaa tagtttagca
     3061 agccctataa atgaaatagc atgtacaagg actactacca atccaaacat tatgtaaaca
     3121 ggtgcttctg acaatccaaa gaaatttgct ttactacctg tagctgcaag gagtaaatac
     3181 ataaacacat ttcctaccag tgtactccct ttaagctttg atatttttgt catacccaat
     3241 aatattgata ctgtagatgc aacaagtact acccacatag tttcattaag tatacctgaa
     3301 gttggaagta tcttggatac aatttgtgca aaagacgcaa ctccaaaccc aacagaaagt
     3361 aaaatcataa gttctacaaa cgtaggtgcc gtattatctt tattttcttt ttcaagtttt
     3421 gatgtaacct catctataaa acttgtatcg cactttgtcc atttgttaaa tttaggtcca
     3481 aatttcacag acattagaca aacagacatc catactgtat atcctataga acccataagt
     3541 attgcataac taagtccttc ttctgcaact cctagagcat tagctgctgc aatcatattt
     3601 gatgagccac ctatccatga accagatgct gccccaagaa ttttccatga atctgcaccc
     3661 attgaatttt taaaaattat gaatgctata acaaatccaa gcataaatgt aaatgtagca
     3721 cagaaaaatg ataataacat tttaggcccc atctttaata tttttcttaa atcacattga
     3781 attaacaaga acactaacat taatggaagg aagttcactg ttattatgtt ttgatacgtt
     3841 ccaacatctt cattctgtgc aaataaacca aatgatccaa gtatagctga acctaagtat
     3901 attaaaagca ttggattaat aacatcaaat accttccatt tcaatttatc aaccataaaa
     3961 aaaattacac ctacgaatac aaccaagaaa ctacaatata caaatccagt ctcaatcaca
     4021 ttatacacac cctttctttt cctaattaat ttgattttta tgatttctaa gtaaagactt
     4081 tttcagataa tagacaatta aaataaatac ttttatctat atcatgctaa ttaaatacat
     4141 atatacctaa ctattgaca
//
LOCUS       DACTGW010000266         4149 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.269, whole genome shotgun sequence.
ACCESSION   DACTGW010000266 DACTGW010000000
VERSION     DACTGW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.269"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(218..478)
                     /locus_tag="I9573_003330"
     CDS             complement(218..478)
                     /locus_tag="I9573_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HAT4797918.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMGKFQDLVKITSKK"
     gene            695..1978
                     /gene="hflX"
                     /locus_tag="I9573_003331"
     CDS             695..1978
                     /gene="hflX"
                     /locus_tag="I9573_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAT4797919.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTSESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGENGITLDVNFDEEDFNIYREYILEK"
     gene            1986..2663
                     /locus_tag="I9573_003332"
     CDS             1986..2663
                     /locus_tag="I9573_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HAT4797920.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEAGEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            2749..3192
                     /locus_tag="I9573_003333"
     CDS             2749..3192
                     /locus_tag="I9573_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAT4797921.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTNCVLAPEFQE
                     GGFYKVKDALDMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            3498..4142
                     /locus_tag="I9573_003334"
     CDS             3498..4142
                     /locus_tag="I9573_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAT4797922.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVVVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITNGTGKIILGEEFYLSEQNGEILI"
BASE COUNT         1669 a          396 c          780 g         1304 t
ORIGIN      
        1 aggaattttt ttttcactca ggaatgatga ttttaatatt aattttgtta gcaagtatct
       61 tactatttgt aatgtataga ttatataaat ctttcaaaat aaaataagat agcaaaaacg
      121 aattatatta aaggttatac aataaaaata gctatgtaat aatatttaaa ctaagagtag
      181 tcgataaata tttatgcata gctatttcta atagaaatta ctttttacta gtgattttaa
      241 ctaaatcttg aaatttgccc ataatgtaat tgtaattatc ttttttgtga ggtaacatgc
      301 aactactgca atctttgata cctttatctg tatacttaaa gtttccacca cagttttctc
      361 ctaaagcata caaaggacaa tagcaaaata gacagttaaa ctcttctggt ttattagttt
      421 tatgacaagg gaaaaattcg caatctttgt gattaaaaaa cttataattt tcgcccatat
      481 tttcctccta attaaataaa atttaaattt tttatactat ataagatttt ggtgataata
      541 taattatact aaataatttt atgaatagtc gataatatag tattattaat catagtatta
      601 atggtataat tagtcaacag aaaaataatg taaaaattat gtaagtgtat tttgtaatta
      661 gaatatgttt tataacaaaa aatgaggtga ttaagtgaaa gaaattcaag aaaaagcact
      721 tcttgtaggt ctgaatttga ctacaatagt gaaaaaaaat gatgatatag acactagtga
      781 atctatggaa gaattaaaag agctaacaaa agctgctggg gcagaagttg taggtagctt
      841 aattcaaaat aaacattctg tagatgcagc ttattatata ggaaaaggta aagtagaaga
      901 aataagagcc tatagtgact ctttagatgc tactttagta atttttaatg atgaattatc
      961 tggtgctcaa atcagaaata tagagaatgt tgttggaaga aaagtaatag atagaactac
     1021 tttaatttta gatatatttg ctcaaagagc acttagtaaa gaaggtaaat tacaagttga
     1081 attagcacaa ttaaaatata gattgccacg actttatgga atgggtggtg agatgagtag
     1141 aacaggtgca gggattggta ctagaggtcc tggagaacaa aaactagaaa tagataagag
     1201 acatatactt aataaagcag cagacataag gcgtgaactt aaagaagtaa aaaagaatag
     1261 ggagacacag agagttaagc gtttaaagtc aaatataccg attgtagcac ttgttgggta
     1321 tacaaatgca ggaaaatcta ctcttttgaa cgaattaata aagacacaca aggattatga
     1381 acaagaaaaa gaagtttttg taaaagatat gctttttgca actttggatg taactctaag
     1441 aaaggctctt ttgccgaata aaaaagaatt tttagtagta gatactgttg gatttgtaag
     1501 taaacttccc catgatttag tagaggcgtt taaagcaaca ttggaagaag tacagtatgc
     1561 agatttaatt ttacatgtaa ttgatgcgac aaatactagt tatgaattgc aaaaaagtac
     1621 gactgaaggt gtattaaaag aacttggtgt aaatgataaa aaacatatac ttgtatataa
     1681 taaagtggat aagctagaat tggatatata tcctaaaagt caagaagata ttgtgtatat
     1741 ttctgcaaaa caaggcataa atatggataa gctattaaat atgatagaaa ttgctttaat
     1801 ggaaaatact tattcagtaa gcttgatgtt accttatgag agaggtgata tatttagtag
     1861 aataaaagat aaatataatg tggagaattt tgagtatggg gaaaatggca taacgctaga
     1921 tgtaaacttc gatgaagaag attttaatat ctatagagag tacatactag aaaaataggt
     1981 tgaatatgga tataaattgg gaaaatatag ataatcttga agattatttt ataacctatc
     2041 ttctttataa agaatctaaa acagtatctc aaattagtaa aattagaaat atttcaagta
     2101 ctgaagcagg tgagcaactt atacaagcaa aattaaaaat aaaggaaatg caaaaagatg
     2161 attttgaggc atctaaggat attttagata aatttttaga actagataaa attaagagac
     2221 ttgattttat ggatagctta gatgacgaaa aaatggtata ctttaagaga aaggttttca
     2281 aaagaatcct tgtggaaaaa aatgctgaag atttaattgt tttaatttgg gcgacaggtg
     2341 agctaaaaga tgatagattc ttaaagttgc ttcatcagct cacaaaccac agacactcag
     2401 atattagaag aataacttat tcagcaatca gaaagattga atcaccaagt agtagagaag
     2461 tactgcaaaa aggtctctac gataaaaatg cacaaactag acagtattgt gcaaaggctc
     2521 tttcaaaact aggggatgaa aacagtttaa agattttaca gcagttgaaa gacaagaata
     2581 agaattttga aaaagaatat gtgcttagag cttatgacga agccattaag tcattggaaa
     2641 atgcaaaaaa tttgaaaaag taatttttta ttaaagcaat ttgtttactt aatgatgtat
     2701 ttctagagtt taacaaggta ataaataaat taatgggagg ggtttcaaat gatagtaaga
     2761 cgttgtgcag gtggagtagt gttttatgct aataaagtac ttattgtaaa aaatgatagg
     2821 ggagaatgga cacttccaaa aggaaagata cttggaggag gacttccata tgagagtgct
     2881 gttcaaagag ttaaagtaga aactggcata gatgctaaaa tgatagatgt ggctggcgat
     2941 actatgtatg aatttttctc aagaagtaga caacaagaag tttgtaatgc aataatgtgg
     3001 tatgttatgg aagcttgtaa cactaattgt gtacttgctc ctgaatttca agaaggtgga
     3061 ttttataaag taaaggatgc tttagatatg ttatcacatc ataaagagca agctttagta
     3121 gaagtatctt ataaaaagtt taaggaatta aagaaattag tttcagaggg cgatgatgtt
     3181 ccagcaaatt aattgtgaat ttagaggatt ctgaggaagg gatagcccct tcctttttta
     3241 attgaagcag ttttaagcgt aaattatata gtctctttaa attatgattt atcaatttag
     3301 agctttagta gtttatattt aaaatcagta agatatattt aaaataaaaa ttaaaaagta
     3361 tacaatttta ttatataatt gtcttatgat attgattaaa gaagtattaa aagtccattt
     3421 atttttaaat agaataattt tatagatata taatttgatg tgtatattag aaaatgatta
     3481 aaataggagg aaatacaatg agcaattaca ggacattaca tgaatttgga acagatgaaa
     3541 taactattga aaagtctgtt tttattggat atgctaagcc tataaagagt gaagaagaag
     3601 cattagagtt tataaatgaa ataaagaaaa agcataaaga tgcaactcac aatgtgtggg
     3661 cttatactgt aggtaaaaat atgaatatac aaagatatag tgatgatgga gaaccacaag
     3721 gaacagctgg aatacctaca ttagaggtta taaaaaaaga agatttgaga gatgtagtgg
     3781 ttgttgttac taggtatttt ggaggaacaa aattaggtgc aggcggtcta gttagagctt
     3841 atacaaaagg tgctaaattg ggacttgaag caggtaagat aatttataag gtaatgtatc
     3901 aagaagttaa ggttaagata gactatactc aacttggaaa agtgcaaaat gagttaatga
     3961 atttggggta ttttataaaa gatactgtat atgaggataa tgttgagata gtagtgtatt
     4021 caagattaga agatgtagag aaattatcag aaaaaatgat tgatataaca aatggaacag
     4081 gaaaaataat tttaggagag gaattttatt tgtctgaaca aaatggagaa attttaatat
     4141 aatctaaaa
//
LOCUS       DACTGW010000267         4137 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.270, whole genome shotgun sequence.
ACCESSION   DACTGW010000267 DACTGW010000000
VERSION     DACTGW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.270"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..563)
                     /locus_tag="I9573_003335"
     CDS             complement(<1..563)
                     /locus_tag="I9573_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HAT4797923.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIV"
     gene            complement(635..1243)
                     /locus_tag="I9573_003336"
     CDS             complement(635..1243)
                     /locus_tag="I9573_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HAT4797924.1"
                     /translation="MDSNDKKGLKSTEIIIINGSPRINKNCSFIANKVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRNKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(1251..3014)
                     /locus_tag="I9573_003337"
     CDS             complement(1251..3014)
                     /locus_tag="I9573_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HAT4797925.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDTIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMKDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(3057..3518)
                     /locus_tag="I9573_003338"
     CDS             complement(3057..3518)
                     /locus_tag="I9573_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797926.1"
                     /translation="MLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKIDS
                     VFFKLLKLAFGSILLMIAFNLNMILGIGVFLIGVVYILYKRNMEEQVKEHINNVKNNI
                     DISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            3707..>4137
                     /locus_tag="I9573_003339"
     CDS             3707..>4137
                     /locus_tag="I9573_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797927.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQHFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGN"
BASE COUNT         1402 a          705 c          393 g         1637 t
ORIGIN      
        1 actattaatt ctactaaatc tccattttta acgcctgctg catttccctc ttccatatca
       61 acatgcattt ctagattaaa gctatcacta gctcttacta atacattttc aaatactaat
      121 gctcttggtc cagatgtttt aacttttact atttgcttat cagttacacc aaatttagca
      181 gcatcttcat tgctcatatg tatatgtcta gaagcaacta taactccttc tttcatttca
      241 acttctccag caggtcctac taatttaaca cctggagttc cagctatatc accagattct
      301 tttattggtg cttttactcc taatgggaaa ccatctgcta aagatatttc aacttgagta
      361 tgagctctag ctggtcctag tactctaact ccctttaaag ttccttttgg tcctactaaa
      421 tccacttttt cttcacaggc atattgccct ggttgagata atggtttaaa tttagttaac
      481 tcatgacccg ccccaaataa tacgtctata tcagcttgac tcaaatgaac atgtttatta
      541 gataatgcta ttggtaattt cattgaaatt ccctcctttt aattatttaa attattgcaa
      601 tacaattata cataatatta cctacaaaat caaattatat tgaatcaata tatatcttta
      661 aatttttata tattatatct ttaaattctt gattatctat ataagataca tcatctgtat
      721 tgtttaaata tatattgtgt aaaagtttag tatttatact cttaaagaaa aaatccataa
      781 caatctgtcc acctttaaac tgtgaatcat atgatttact cccccccact gcaatataca
      841 ttcctatccg tttcttattt ctatcaattg aaggtttatt taatacatat ttacttgcat
      901 atatagcttg agttctatca acaacagtct ttaatttagc tgatattgaa tcaaaatgta
      961 caggacttat tactatagtt ccttttgaat catcaaacat tttatataat ggcttcatat
     1021 catcattaaa cttgcaatac cctgtttttt cacaaaatcc acatgctgtg caatattcaa
     1081 tatccatatt gtagatatta aatattttac aacttaaccc acactcttct agcatagctt
     1141 ttactttatt tgctataaaa ctgcaatttt tattaattct tggacttcca tttattatta
     1201 taatctctgt acttttaagt ccttttttat cgtttgaatc cataatcaat ctacctatcc
     1261 tttaacataa ctattaaaat atcatcatca taattatctt ttgatgagaa attctttaaa
     1321 tccaatttta gattttctac tattaactgt tgatttaatc taaaattttc ttttagaaag
     1381 ttttctaatc tgtttatacc atactcttct tttgatgaat tttttatctc taaaattcca
     1441 tcagtgtaca tacataccat tgcataatct tccattgaaa gagtattatt ttcataagta
     1501 gcatctttca ttattccaat aggtatgccc tttttacaat ttaaattatt ttctattgtg
     1561 ccatctttct ttacaactac aggactatag tgacctgcat tagacacagt aatcatattt
     1621 tctttagtat ctataatacc tatcaaacaa gttgtaaata ctcccatctt gtcaaattca
     1681 tcatataaca tcctatttaa atttgtcatt atttctcttg gtgtattgta ttgattgcat
     1741 aatactttaa aagcaccttt tatcattgca acaacataat ttgataccat tccatgtccc
     1801 attacatcag caacaatata aactatatgt tcgtcatcca cttttgtagc ataataaaaa
     1861 tctccaccta ctacccttgc tggtttatgg taaaaataaa gttctttctc atcattacaa
     1921 tccattttac ctttatccat aataagctgt tgttgtttat ttaatatgtt caactcattt
     1981 tcaagaactt tgtgttttac ttccttttca ttatgttcat ataattgcat cccaacagaa
     2041 acttgtttgg acaatatact aagaatattt aaatcatcac tagtatagtt tttactattt
     2101 gcacaaacta tacatcctat agtctcattt ttatcctgaa gtttgtaata tatataactt
     2161 tccaaattta tgcccttttt tgagcatggt tttaaattat ttaggtatcc ccctaatgac
     2221 tctttcttta aaaacatatc aatcatagga tataaatttg ttttcttttt ttgctcaaca
     2281 ttataacaac taactatagt atccttatta tgatgactat ttataattat taaagcttct
     2341 ttactattag taatattaca cgcttgttta cttaaatttt ccaaaaagcc atcaatactc
     2401 ttattaaggt atagtttttt agtagcttca tttatggcac ttattgagcc tcttagttta
     2461 tcaattaaaa tttgtacatt attattttta ttagttattt ctattgataa acttaagagt
     2521 gatgcaacag atgatataaa atctatatct tgttctgtta tataatcatc ctcttttaaa
     2581 aaacaagtca taaatccaac aactacatta tttattataa aaggaaaaac tattctccca
     2641 atataacctt cagcttttgc taaatctcgt tcaccttctg ctctagaatc ttcaaataca
     2701 ttttccacat atattatctt tttttcattt acagcctcat gtatatactc tggatatgtg
     2761 tcaaaatcaa tttttgcacc tttaagacta tttattggaa aaatttgagg tacatattct
     2821 aaggtttgcg aacatactaa gtaggcatat ttagaatcat ttttataaaa tagatttaca
     2881 catgcctttg tagggtgaac aacttctaac attttttcta ttatgtcatc ttttatatta
     2941 aaaaaatctg tagattccgt caccatagcg gatatctcca ctaaattctt cattttataa
     3001 ctgccattag tcatactcaa tttctccccc tatatattaa actatttatt agtattttaa
     3061 catacattct ttatagtaaa aacaaaaaca acgacaaaac tagcaactaa tatccagtta
     3121 aatcctgtca ataatcctat aagaagtaat gtaatcaatg catttagacc tgttctccct
     3181 ttttcatcca taaaagttat tttagagatg tcgatattat ttttcacatt atttatatgc
     3241 tcttttactt gttcttccat atttctttta tatagtatat aaactacacc aattaaaaac
     3301 actcctatac ctagaatcat gtttaaatta aaagctatca taagcagtat tgaaccaaaa
     3361 gccaacttta aaagcttaaa aaatacgcta tctatcttta gagttatatt tctgatttct
     3421 ttacattcat ctcttaaaga atatccactt gagtctaggt tattaatctt tgtttttaca
     3481 atttcagaat aaaaaagctt tattatccca tttaacattt tcatcactcc taaatatttt
     3541 acatatattt gtaagtttta caattataat attttttata cttggtaata ataaaaaata
     3601 acaaatattt tatgatttag tatttactgt aaataaatta ttgaagcttt attagttaag
     3661 tatagatttt taataaatat ttttataatt agaaggaagt ggttttgtgc aaatagaaca
     3721 actcgattta gaaacgagaa ataaaatata ctgctatact aaaaaaattc ttagaaaata
     3781 tcaaaaaggc ataacatctg gaaaattaac agcagataaa tttgcagata atatattgtc
     3841 tcaacatttt atcagtagta tacttaatga aaaaatagtc aatgagacaa attttaagat
     3901 atcttataga aactatatag aaactttaat aaatattcaa aatgaaaatt tatctaactt
     3961 aagaaaaaaa tctactaaaa ctgcaaaatg tttcaatatc tctcaaataa cccaattaaa
     4021 aaacttacta agtaatactg gttataattt attaattcca tataaatact taactgctag
     4081 agatatagaa ggaatcgtta ctcttattaa tactgggtct attgaacttg gaaatga
//
LOCUS       DACTGW010000268         4132 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.271, whole genome shotgun sequence.
ACCESSION   DACTGW010000268 DACTGW010000000
VERSION     DACTGW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.271"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            385..1659
                     /locus_tag="I9573_003340"
     CDS             385..1659
                     /locus_tag="I9573_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAT4797928.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSAIYVKNATEGINF
                     LAKFFKDENPDKFIITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1866..2183)
                     /gene="trxA"
                     /locus_tag="I9573_003341"
     CDS             complement(1866..2183)
                     /gene="trxA"
                     /locus_tag="I9573_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAT4797929.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2450..3202
                     /locus_tag="I9573_003342"
     CDS             2450..3202
                     /locus_tag="I9573_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAT4797930.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKN
                     KSVEYVLNIKGDKNILKKALEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHTHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3189..4070
                     /locus_tag="I9573_003343"
     CDS             3189..4070
                     /locus_tag="I9573_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAT4797931.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGRLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNYRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
BASE COUNT         1620 a          542 c          567 g         1403 t
ORIGIN      
        1 attatataca atatattttc cattatgttc taatcaattt ctttactatt tttacaactt
       61 tattagtcat attcataatt atttctaatt agtgtttaac attttatgta aaatttataa
      121 aaaaatgtat ataaattaaa ataagtgact atactaaaaa tatgatacgg ataaacaata
      181 agttcaacct atctccccca aaatcaagtg agctacagtt tcccccaact gtagctcatt
      241 ttcgtgtaat ttaataaaca tctcaacaag aattaattaa caaaacccct tataaaaaca
      301 taacatatat taaacaagct cttgattaaa acttgagctt ttttaataaa aaacagaata
      361 gtttattaga taaggggttt ttttatgcaa ttcatggatg tatctaaata cattgaaggt
      421 gcaaacctta aagttaaaac taaaaatggt gaaactgtta aaagaatata ttttaacaac
      481 tctgccactc cattagtttt aaaaaacgta gtggataact taaactgtga gattccttgg
      541 cttacttata ttaatgctcc aggaataatc tcagaaaaaa atactttaaa atatgaaaat
      601 gtacgctcta ctatcctcaa acttattgat ggagacgaag aaaaggattc tgctatttat
      661 gtcaaaaatg ctacagaagg aataaacttc ttagcaaaat tttttaaaga tgaaaatcca
      721 gataaattta ttattaccac agcaatggag catatggcta attacctacc ttttaaagtt
      781 aattttccta caaaagttgt tggaattaca gaaaaaggag aacttgattt atgtcaatta
      841 gagaatatac ttaaaaatta tgctggtaat gtatctctcg ttacagttac aggagctagt
      901 aatgtcacag gtataaccac tcctatatat gaaatagcaa gaatagctca taagtatggt
      961 gcaaagatat tggtggatat tgtgcaagta attcagcata aacctttttc aatgatgcca
     1021 catgaatgtg atgagcatat agattttgtt gtttttagtg ctcataaatg ttattctcca
     1081 ctaaatggag gagctctggt aggacctaaa aaattctttg aaaaatttaa tcctgtacta
     1141 tatggctcag gttctaccaa atttgtaaat gataacaaaa tactatatgc aaacgtaccc
     1201 caacgttttg aagcaggata cccagagtat tttggaactc ttgcaatggg aaaagcactg
     1261 aacactttaa ataaaattgg tctaaacaga atttcaaaat acgaaagaga attattttta
     1321 tatgctaaag aagaaatgag taaaatacct aatataataa tatatggaga taaatcaaat
     1381 aatgtaatta tcccaaatat atcttttaat ataagtaata tgtattacaa agatgtagca
     1441 aaatatttag ttgataactt tggaatagaa actggagctg gagtagtagg tgcagatatt
     1501 tatgtacaaa aattattagg tataagtcca aaagaagcat acgtcagatt tatgagggaa
     1561 aatcctgtag gtcttgttag gataagctta ggaatgtaca atacttttga tgaggtggat
     1621 agctttatac atgctattaa aactttggct tttaaataag ctttttctat tcttttttat
     1681 caataaatag agagtagttt tgttttcaca aaaaaacaaa ttatatcaac agtcaatttc
     1741 attagcaatt agacactcat ttgtaactat acaaactatt ttacactata caaataaaaa
     1801 actcattaca atgagttttt ttgtttatat atgatattta ttttaattac ttcaatttac
     1861 tttaattaca tatatgattc aattttgttt tttaaattct ccttaggcat aaaacctatt
     1921 aatctatcta caggttttcc atccttaaat attagcatag ttggtactgt agatacttca
     1981 aatttctgag ctaattctat actttggtct atatccacct tcaagaagtt tgctttttca
     2041 accatctcat ttcctaaagc ttcatatatt ggtgatagca tcttacaagg tccacaccaa
     2101 gtcgcaaaga aatctacaac tactaaacca tctttatttt ctacttcatt tataaattca
     2161 ttattattta ttatttttac cataatatac atctccttta ttatcttctt gatactaata
     2221 tactacgccc tatataacag ttcaattagg cacttttttg taaccgccta tttttaatag
     2281 atttaatatt atgataaata gatttataag attaaatatt tataagacta aatatttctg
     2341 atatatttta tttttttctt aatatagttg acattttctc ccttaaatta taaaatcaaa
     2401 ttaataacta cataggtagt tactaatctt ttatcaaagg agtttctata tggataatat
     2461 aattaatgaa ttacaaaata taggatttac taaatacgaa tctcaaatat acatatcatt
     2521 attaaaggaa agccctttaa caggatatga aataagtaaa ttatcaggtg taccacaatc
     2581 aaaagtctat gaaaatataa caaagctatt aaacaataat ataattatca atgttggaac
     2641 tgaccctatt aagtacatac ccataaaccc taatgaatta ttaaaaaata agaaaaaaga
     2701 atttgataat tctatggata atctaaagaa tagcttacag atattaaata aaaataaaag
     2761 tgtagaatac gttttaaaca taaaaggaga taaaaatata ttaaaaaaag cattagaaat
     2821 gataaaaaat gcaaaacaag aaattataat ttcttgccct tatgaagaaa ttcttgaatt
     2881 aaaaaaagaa ctcctaattg cttacaaaaa aaatattaat attgaaatac tacttttaga
     2941 aaacaaaagt ctgaaaggtc ttgataatat tcatactcat ggaggtaata aaactacact
     3001 tgaaaataaa atgctttaca aaggaattat tttgattgta gataatgatg aaatattgac
     3061 aggaaactta tcagaaggca gtgaggccat ttcaatttgg acaaaaaact ctaacatttt
     3121 atatattgga gttcaataca taaaacatga aatttatatt tcagaacaaa taaaaggagg
     3181 ttataaaaat gaacaaaatt aagattaaac aagttgatgc ttttacaact ataccttttg
     3241 gtggaaatcc tgctggtgtc gttactgatg cctctaacat ttctgatgaa attaagcaga
     3301 atgtggctaa agaaatgaat ttatcagaaa ctgcatttgt ttcagagtca aatgttgcag
     3361 attttaaggt acaatttttc acaccaggtt tagaagttga tttatgtggt catgcaacaa
     3421 taggtacttt ccatgcactt ttggaagaag gaaggcttaa tactaataag agtatatttt
     3481 atcaagaaac aaaagctggt gtattatctg tagagcttaa ggaaataaat aatgaaaata
     3541 tatttatgat ggaacagaat acacctgaat ttgaaaattt agataattac agaaaagaac
     3601 ttgcacaaat gataggtgtg catgaagata gacttcttga atatcctatt ataaaggtaa
     3661 atactggact ttggtggctt gtatttggtc taaaatcttt agatgattta aaaaatatct
     3721 ctccagattt aaataaaatc aaaaaattta gtggtgataa taatataatt ggtataactc
     3781 cattttgtat agaaactata gataaaaagt gtgactacca tttaagatct atagcaccat
     3841 atgttggagt tacagaagac cctgtatgtg gaactgggaa cgcttgcgta tcatcttata
     3901 tagtacatca caatatttta gataaaacta gttttattgg tgagcaaggt aactttataa
     3961 acagacctgg aaaggtatat gtaaatataa ctagagaaaa caatgacata acttctgtaa
     4021 aaattggagg aagtgcttat actgttttaa gtggtgaaat tatgtattaa tcccatacct
     4081 aataaaaaca aataaaaata ctagctaact aaagctagta tttttatttg tt
//
LOCUS       DACTGW010000269         4104 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.272, whole genome shotgun sequence.
ACCESSION   DACTGW010000269 DACTGW010000000
VERSION     DACTGW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.272"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(252..1313)
                     /gene="ytvI"
                     /locus_tag="I9573_003344"
     CDS             complement(252..1313)
                     /gene="ytvI"
                     /locus_tag="I9573_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HAT4797932.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLSLILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFLELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFIGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            1587..2327
                     /locus_tag="I9573_003345"
     CDS             1587..2327
                     /locus_tag="I9573_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HAT4797933.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            2339..>4104
                     /locus_tag="I9573_003346"
     CDS             2339..>4104
                     /locus_tag="I9573_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAT4797934.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFV"
BASE COUNT         1765 a          357 c          639 g         1343 t
ORIGIN      
        1 agattgaaga aaaaataaaa gaaaaattta aaaatataga tgttttaaat aatgaaaatg
       61 aatataaaga aatgattgat attatttgtg cagagtagaa aaaaagggag tgtcttagaa
      121 tagacttttt agtttatgag gcactctttt tgtataaaat caaatgtatt ttcatcattt
      181 taacaattaa aaagagctta tccctgtttg agaaagccct ttttaattta aataaaaatg
      241 tcaaggttaa actatttaaa tgcagtaata atcccattat catgtaagtt ttttatgatt
      301 gtagctgcga tagggaatcc aaataatcca gctattccaa atagttgagc tccaataaac
      361 atacacatta atgttattaa aggatatagg ccaatttggt gtcctactac ctttggttct
      421 aatatttgtc taacaacggt tataattatg tatagtatta aaagtcctat tgctaaatta
      481 atatttccat taatgagtga gataatcatc caaggtgtca aaatacttcc tgtacccaag
      541 actggcaaaa tatcaacaag tgctatgatg acagatatag taaatgcatt ttcaaccttt
      601 aggatagtaa gtccaataag tagttctaag aatgtcactg atagtagtat tgcataggct
      661 tttagtagtt ttaaaagagc attaatactg ctatttttta tatttattat cataaattga
      721 cttttttctg gaaattgttt taagataaag tttactattt tttgatagtc aaaagtaaag
      781 aaaaaagaag caattaaagt aataataagt tttaacataa atgcaggtgt tttacttgca
      841 atactagcaa tagcattgag tgcatttgtt gaaatagaag ctactaaaga catcatagat
      901 tgacttatat tatcccaacc aagtattaat gataggttaa cattaggtgt actagcatct
      961 atctttgaaa ataaagtatt tagcgttggt tgtatacttg atttatatat ttcaggaagt
     1021 ctaaaaaata catcacttac acttgcaaat atcttcacac caaagctaac taataaaaat
     1081 atacacaacc cataaaaaat taaaagtatt actatagaaa taagtgtacg atttaaattt
     1141 gtattatttg taataagtct tataataggt cttaagatgg atgcaatgat aaaactgatt
     1201 acaaaaggca ttaataatgg caataagaat tttaatacaa tatatacaat acttgttatt
     1261 aaaactacat atgtaaattt aattataaaa ttcttttgct tttgaatatc cataatataa
     1321 aacctccaat gtatttgata ttattctaaa tatggtcttt tttacagaaa atatactact
     1381 gtatattgat tataaactat aaagcttatg taaagtaaat aaattatgta aaatttaggt
     1441 taattttgta taaagagata cttttatatt ttacaattaa taaccaaata ctaaaaaata
     1501 ttttaaataa agaacttaaa attgtgataa aattaattta tagatattaa aaaaagtata
     1561 atatatggtt tttaagaggt gctaagatga aaaattttag agatttaggt gggaacaaaa
     1621 ctgaggatgg cagaactgta aagaagggat tattttatag gtctgcaaaa ttatcaaatc
     1681 taagtgaaaa tgatataaag attttaaaag agttaaatat taaatatata tttgattata
     1741 gaagtgatga agaagctcgt aaacatccat caacaataat atcaaatata aaaaatataa
     1801 gaataccagc aatgagagag ctagaagaat caggaggaag ttttggttct attgaagata
     1861 tgattgatgg attgtttgaa aaagatggtg catttaatat gttaaataat agttactata
     1921 atttgcctat aaataatccg tcatataaaa aattagtaga acttataaga gactattcta
     1981 atttaccaat attaaatcat tgtacagctg gtaaggatag aactggtgtg gggagtgcta
     2041 taattcttat gattttaggg gtatcgagag agaatataat gaaagattat ctaaaaagta
     2101 atgattttgc agataaagag atagaaagat ttatagaata taagcctaaa tttaaggaca
     2161 ttccaaaaga aaatttaaaa tatatatttg gtgtaaatga agagtatatg aaaactgcat
     2221 ttagaagaat tgatgaagaa tacattagtg tagaagcata tttatatgga gaatttaatt
     2281 taaataaaga agaaataaga aaattaagaa atcaatatct agagtaaaag gtgagtttat
     2341 gagtttaaat ataacagcaa gacagataaa tataataaaa atattgttaa attcaaaaga
     2401 tacaattagt ggtattgcct taagtcaaga gataggatgt tcttcaaaaa ctatacaaaa
     2461 tgaaataaag gatattaata aacaacttag aaatggtaaa attttgtcaa taagaggagt
     2521 tggttataaa ctagaaggaa gttttgatga ggtaaatcta aatagcaatc tctatgatga
     2581 tgtagataga gtagaatata taatcagaaa aatattaacc ttaagtaatg aagaaaaaaa
     2641 tactataaaa cttgaagatt tagccgattc tatgtatgta agtgtttcaa cagtaaaaaa
     2701 tgatttaaaa gaagtaaaaa gaatattgga aaagtattca atatcagtta aatctaagca
     2761 taaacaaggt atatgtgttt tggaaagtga agataagata ttaaattgta tagttgactt
     2821 aagcaataag agagataatc aattgagttt aaatgatttt ttaaatgacg atataaagga
     2881 aagaaaacta ttaataaaaa aattattatt agacatatta aatgaaaatg gattagtatt
     2941 aactgatata gaatttaaga gtgtgttaaa taatatatta gttatgttaa gcagacataa
     3001 gtataatgaa aaagaattta tagagaatta tatagaaaat tataggagca aaagaagctc
     3061 aattttagaa aatgaacaaa acaaagaaat tataataaat tcaataaaaa cattttgtaa
     3121 aaatttaaaa ttagctacat caatagatat aagtaatgat aaggtatttg aagagttttt
     3181 atataagcat attagtagtt tatgtaaaaa aataaagtta ggtataaatc aatcacctat
     3241 aatggctcaa gatataaaaa taaaatatcc atttgctttt gaactagcta atatagctaa
     3301 aaagacaata gaaaaagatt tgaagataga aattaatgaa gatgaagttg caaacatagc
     3361 tctacatata ggaggagcag ttgaaagagc atcttataat aataaagata aggttttcaa
     3421 aactataata gtttgtactt caggcatagg cacatcaatg ttaataaaag ctaaattaga
     3481 gaatatattt aaggaaaaat tagaaatact aaagattatt ccttcatact tagttgaata
     3541 tataaaagtt atagatgttg attttgtaat atctactgtt cctattgaag taggagatgt
     3601 gccagtaata aatatatctc caattttaag tgagaaagaa attagattaa ttgaaaaata
     3661 tatagaaaca ggaaatatat acctagattt agatataaaa agcctgtttg atagtgaatt
     3721 attttttact gatttggtgt ttaaaaggaa agaagaagtt atagatttta tggcaagtaa
     3781 acttgtacaa aagggctata tagatgagga tatgaaaaaa tcttactttg agagagaaaa
     3841 aatagcaaca acagaaatag ggaatatggt aactatacca catggagcta ttggaaaaat
     3901 ttttgataat aaaatagcaa ttggtatatt aaagaagcct atttcttggg aaatatcaga
     3961 tgttcgttta ataataatgt tagcccttga taaagataaa atattagatt atgaagtaat
     4021 attttcaaaa atatataaaa gagtagattc tatagcaaaa gttataagta tatgtgaaaa
     4081 tagtagtttt gaaaaatttg taaa
//
LOCUS       DACTGW010000270         4074 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.273, whole genome shotgun sequence.
ACCESSION   DACTGW010000270 DACTGW010000000
VERSION     DACTGW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.273"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(715..933)
                     /locus_tag="I9573_003347"
     CDS             complement(715..933)
                     /locus_tag="I9573_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797935.1"
                     /translation="MKILQQFKPYKYDKEKNIEYDEKIIANQNTEVYIVYPRISDEEN
                     RRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(1212..1367)
                     /locus_tag="I9573_003348"
     CDS             complement(1212..1367)
                     /locus_tag="I9573_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HAT4797936.1"
                     /translation="MALDVKIEVKIIKDVVILNIIHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
     repeat_region   2584..3604
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2584..2612
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
BASE COUNT         1418 a          617 c          366 g         1673 t
ORIGIN      
        1 tttacagtta atatgtatta tagttcttat atttatatat attaatacat ttttatatcg
       61 cctcttcaat ttgagcccca tcattatata aaattaacct acttgatatc ttaatttaat
      121 atatgtacac tattcatatc ttaatagtca atattattca tttttaatag ctctttttag
      181 atgtcagaat tattatattt tgaaatttat taaatttttg cattaaaaaa actagaaatt
      241 aatttaagtc ttgaattact tataactttc tacgtatcta cctttatatt cttactctga
      301 ttttatatct aatattaact cttcatcaaa gctattaaat atacctctaa ttgagtcaat
      361 tatttcactt acttcacctt taatttttat attaacttca accatttcaa atctcatctt
      421 tttatcttat aatttctata agttttttta tttattgtgt tgcttacttt gaccagcact
      481 tttttattta atagtctaaa tgatattttt taccagtttt aatatcctta aatactatat
      541 ttactaaaaa cttataattt cttttaatac attcttatta ctaataacaa tactcaacaa
      601 ttttatcgcc ttttttcaaa tatgatttaa cttttttatt cctaattatt taaaaatagt
      661 tgtccaattt tcttctatga gaaagtgcta agtattagca acttctctat tctcttaact
      721 ttctactgtc tcaactttct tcatagctat attaatagct acttctttag ctacttcatt
      781 gaactcttcc catcttctcc tattttcttc atcagatata cgtggatata ctatataaac
      841 ttctgtgttt tgattagcta taattttttc atcatattct atattttttt ctttatcata
      901 tttgtatggt ttaaattgtt gtagtatttt cataacatca ccccttgtta taaaatatga
      961 actcataaac ttgtcttata cttatttaat tgttacaatt tttacttaaa atcactttat
     1021 attctatatt ttaaagctaa atcctttaca aaagtaacat attcttctat aagtttctta
     1081 tcctcttata tagtatctag tttgtttaac ttatctcttt taaacttaca tacgccttct
     1141 tctgccattc gtcttttatt aattgttaac attgtttcaa gagacacacc aaaactcttg
     1201 ttaagaagct cttagctctc attagtaaaa tattttacat aacttaaatc accttgacct
     1261 ctagcaattt taactgttaa tagacttgtt tcttttttcc aatgtattat atttaatata
     1321 acaacatctt ttattatttt aacttctatt tttacatcta atgccatatt attgctttat
     1381 taatttgtat ccttaaatct taaattttct attaatacat cttctataca gcttagtttc
     1441 tctcgcttaa tcttgaaata tattttctct aaattgtcaa attgttccca agttttatca
     1501 atatctaata ttttgcagtg tctatttgca catctttcga tccaaaggta taattgacta
     1561 gaatgttttg aaatcaactg actattagta ggttagttct taaattcttt caaatactcg
     1621 aattgtaata aaacataatt tacattctct ttatttctat tgaagttagt ttatatattg
     1681 tttacatcat tttcatatat atctgctagt tgctatgcat ttaaaattct cttattattt
     1741 ctttatatta cttgtaaatt attcatcctc atcatcctct taattaaatt tctatcttag
     1801 aaatttaatt tgtaaaaaaa ttttctatat cataatcaag tatcttagat agtttatagg
     1861 taacatatca actctaattg aatattaccc cttcctgtac ttaaaataag ttgatgcatt
     1921 tttaaaacct aactcttttg caacactttg taagtataaa ttttatgtat gtcttctttt
     1981 ttataaaatc atagtgtatc tttttcatat attcacctta attgctatat ttcaataaca
     2041 atatttccaa aacagaaatt tttcgaggta attatgtata gatgtaaatc aaagaattaa
     2101 atatactagg gaaaatatga atagttctca aaaagaattg cttgaaaaaa caaatataaa
     2161 tacaagtgtt atgaatagaa tctggtgaaa gaccaacaag agacgatggg ttaataatat
     2221 ttgctaaaat ttttggttta tatactgatt atgttcttgg tttatcagat acagaaaaac
     2281 taaatattgg caattaaaac tgaattgatt gtattatatg tgtgatgaat ataaatatag
     2341 aattttttga gaagcttgaa atagcttaga aaaaataaga gattcactca agaataagtt
     2401 actaaagatt aaaaaataac aaaagtatct actaaaggat atgaaaatgg tttaagagag
     2461 ttaagtctgg aaatatttat gtgtattctt taaataaaaa tttatttttt aaatgataat
     2521 taaattttta ctgatatggc tatgctttta aaagtcataa ccttattttg atattagatc
     2581 tgtctttaca taccacttag ttaatataaa actaaatata gcttgtaact cctttgatgc
     2641 acttaaaagc tttacatacc acttagttaa tataaaacga atttagctat agaagcatca
     2701 aattgtggca cagcacttta cataccactt agttaatata aaacctttaa agacaaaacg
     2761 tcgtataatt aaatcatagc aactttacat accacttagt taatataaaa ccttataatt
     2821 ctttaagtac tagactgagc aatttaatct ttacatacca cttagttaat ataaaaccat
     2881 cccattcagc aacaacagtc gctaaatctt ctgcctttac ataccactta gttaatataa
     2941 aacttttagt atatttttta ggtctgccct ctttaaaact ctttacatac cacttagtta
     3001 atataaaaca cgaggttata gttaattttg tcgtgggagt ttataacttt acataccact
     3061 tagttaatat aaaacaattt atatatatat atatattaaa gtcagacccc tttacatacc
     3121 acttagttaa tataaaactt gttgttcagc attagcaatc atatcatcta ttcctttctt
     3181 tacataccac ttagttaata taaaacctgt accatcagag acagtcatta aaagatttat
     3241 aaactttaca taccacttag ttaatataaa acttataaat tgtctctgta cccattttat
     3301 tcaatgtcgt actttacata ccacttagtt aatataaaac catgaaaaaa tgggtaattc
     3361 aaactctttt attttcttta cataccactt agttaatata aaacgttgga gaaaataatc
     3421 cttcttatga tttgccaagc ggctttacat accacttagt taatataaaa ccagcggatg
     3481 aattatttat gttaattgga gatggagact ttacatacca cttagttaat ataaaacttt
     3541 caatattgaa tacaattaaa ttttcaaact catctcttta cataccactt agttaatata
     3601 aaaccccaaa ataaattgag tatttccaac acttacatct atacaattct ctcaaatttg
     3661 cagtgaacca tgagtagtgc aaatgataac atttatcaca taccctcaat gccttatatt
     3721 tcaactgtta ggccctattt tatcaaaaat atcgctcact gcaaaactat ttataatttt
     3781 ataatattat gaatatatta tttttaaata tctgtaccaa tttgtagtat aataaaatca
     3841 agaaaataat ttactcctat aaagagtgat tatttttaag tgaaaatatt atttttttaa
     3901 atcatcctta ttggcgtttg ggagattttt attttatcat aaatataaac tgatataaca
     3961 ccagctgaca tgcttgctgc tttcgattat tattcttgct acaattatta taacatataa
     4021 aacttactta tttttaccaa atattttata caaacaataa atattaactt atga
//
LOCUS       DACTGW010000271         4001 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.274, whole genome shotgun sequence.
ACCESSION   DACTGW010000271 DACTGW010000000
VERSION     DACTGW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.274"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(497..907)
                     /locus_tag="I9573_003349"
     CDS             complement(497..907)
                     /locus_tag="I9573_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797937.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(1123..2505)
                     /locus_tag="I9573_003350"
     CDS             complement(1123..2505)
                     /locus_tag="I9573_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HAT4797938.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKSGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIEQSNYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2516..2875)
                     /locus_tag="I9573_003351"
     CDS             complement(2516..2875)
                     /locus_tag="I9573_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT4797939.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(3080..3859)
                     /locus_tag="I9573_003352"
     CDS             complement(3080..3859)
                     /locus_tag="I9573_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAT4797940.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         1403 a          651 c          386 g         1561 t
ORIGIN      
        1 ttgatttttt taaacaaatc ttaaaaatcc taaaatgcta attttcttta acttttaatg
       61 aatctaatag ataatattta attttcgtta tatttttcag aatgtttcaa ttattatttt
      121 taagaaaaaa aaataatgtt aacatttctt aacatacatt aaaaaatgtt aacattataa
      181 atttggttta atacttttac tttgactcga tttactttta ttctacttct tactttattt
      241 ttaacttaga ctttattttt ttattactta tttgagtctt atttatttta ttctcactcc
      301 atatgttcaa ttacatttgt ctaaattctt tgatactttt attacataat tttaacaagc
      361 ttaagtgtta tttgggtagt gcagttgata attatttaaa taatcatcaa ctgcatgttt
      421 atatcattac agtagcttaa tgtatttaat catacttact taggattttg aaatatctta
      481 gagattatat ttaacattag tcaattgctg cataattgta agcaattgaa atttgagacc
      541 catcttcatt ttctatagtg ataggcttca taatttttaa tccttcagtt tttatctttt
      601 ctaattcttg ttccattttc ttaattgttt cctcaaggtc tttttgactt acatctttga
      661 ctgcattagg gtctttagca tagtctttta attcttgaat atgtttagca tactcttcat
      721 aagtataatg ctcaacacca tacttttcca attcagtagg gtcagttatt cctgatttta
      781 ttagagattc aagtttaggt aaagcaatat tagatgaatc atttccattt gcaaatactg
      841 ctccagttcc tcctagtacc attgcagata ccatcattgc acttataagg gattttttta
      901 ttttcataac caatcttcct ttcttagaga tttatttggg tagtgtagtt taaaatgact
      961 attgcaatag ttatcaacca catctttaga ctaccatagt agtcatattt gtgtcaagtc
     1021 tttttaaaaa acttttcaat aaaaaatact atctcaaaac aaaattaatt attttgagat
     1081 agtattttaa ttctaaatct tttatattac taagatttat ggttactcta tccatgttct
     1141 atattgttct ttattaactg ctaacaaatt cccttttctt gatatgacaa agcgattatc
     1201 attatagtta ctttgctcaa ttgaaacttc tcctgaatca gtataaagta catccttact
     1261 attactagat ttatttacat acgtttcttc actattctca gaattagtta ctttgctcat
     1321 agatgattct tcatgctctc taaattcaat tacagatact tttgccgtac taaaatgagt
     1381 ctctccatca cttttagctt tactaacaag aatatcagat tctactttgg gagaatataa
     1441 atcagttgat ttactactta ttgaaaatcc acttttatca ctttgttcat tttttgttaa
     1501 accaaaagat tcaacctcat taatctcttt attttctgtt gcaaaaacaa gacctgtccc
     1561 acaaactaat acagttgaaa ctaaaaaagt aacataagtt gattttttaa atttcataat
     1621 attaactatt ctcttttcta tttttgtctt tccaaatcca ctacatattg tcattgaaaa
     1681 tggattattc tcctctgcta gacttattag tgtggttgca taatcagccc tatattcttc
     1741 accaagtata ctcataacct tttcatcaca actaaattct atgtctttat ttagtaaaaa
     1801 aaacatcacc caaactaagg gattaaacca atggacaaca agagctacaa gcataaataa
     1861 ctttaacaga ttatcaaagc tattaatatg aaccatttca tgagttaaaa caaagtctaa
     1921 agtttgatta ttagcataat tcatttgctt tggcaaaata attcgtggtt ttaaaatacc
     1981 agatgtaagt ggtgtcctag taaattcaga tatgcaaaca ataaatcttc taaaacggct
     2041 attcttacac atccattttt taatgaactc atgatttttt atatgaattg aagttttaaa
     2101 aagattaatt attttaaaat atctaaagca taaaagtatg ctaaacataa tagtaactat
     2161 aaaagacaaa atgcccaaca tttccaacat cataatattt tcaatattag acattatttc
     2221 aaattcgtca gaattgtttt tacctgtaga gtcactagct ttcttaagag tataattttc
     2281 tgttgatgta ttacccaatt ctacatcaaa agtcgattca attatactat aaatactaaa
     2341 accagaattt aaacttactg gtattagaag tttaaataaa acaatcaacc acaataatac
     2401 aaataccttt ttaggtaatt ttttaaataa acatgctctt ataaaaataa ctattaaaag
     2461 taatactcca gaagtaaaac tcatttctaa caatttaatg aacataaatc ttacctcact
     2521 ttaatttttc aacaatttgt ttgagttttg taatctcatc acttgaaaga ttttctttat
     2581 taataaaagc tgaaagaaat ttttctgaag accctccaaa cattttatta attaaagttt
     2641 ctgtttcctg tttttgtact tcctctttgc ttattaaagc tttacaaaca aaatttggct
     2701 caattctctc tattgcacct tttgacacta atttttttat aacggtatac gtagtgtttc
     2761 tattccatcc tgtttcttct tttaaaattt ttactaactc tccagccttg ctttctcctc
     2821 tctcccatag aacttccatt actttaagtt ctgaatcaaa taattttatc tccatataaa
     2881 atcgctcctc aaatatagtt atcacaatag tcattactat aataaaaaac tatcatagta
     2941 gtctatacta tgtcaatcat ttatttttta ataataaaaa ctgtcttttt aaaaaattct
     3001 tataaaagac agttttatca atgttatact ttaatgttta attaaagcca tattttattt
     3061 aaatgtataa ctatctatct tagtacatag tataagattt ttctttatct aatagtatat
     3121 cctctacatc tgggttttct tttactaatt gtctataaaa attacaatta gtaacaccta
     3181 catatggaat tagccatagt agacctattc caagagtagc tattgaaagt atataccatc
     3241 ctataaaact aaagtttagt acaaataatt taagtacatt tcctttcatc atatctaaac
     3301 tagcacccat tgatttaaat acacccatat tatccttgtc ttcacaaatt aagaaagtag
     3361 cttgagaaac atataatagc aatatataaa ccggtaacat aagaacaagt attataagaa
     3421 ctagagttat tccagtagaa ataatagtcc ctattccaac attatcactt gataaatatt
     3481 caattgctcc actagtaagt aaaactccaa atgataaaaa cgctcctata attgtgatta
     3541 taaaagaata aatataaaca ataattgatt caaataaata aaaaccaaaa cttcttaaat
     3601 aaattctccc acttacaaag atatctccta tcttgacttt ttcatcttta gtaattttca
     3661 agaacataca tgtaaatcct gctgaaacgc aaaaagctat tacagccact acaatagtag
     3721 tcaatggttc aaaaggtaat cctacaattg atattaatac tatcaatgca atatatactg
     3781 ccatagaaat tgcacatatt ccccagtttc cagataactg agctttagat actcgtttta
     3841 attcttgcct tgataccata aaaaacaccc ctttcatttt ataaaaaaac ttttaaaaat
     3901 ataaataatc ttttaacgta taatattaat atatcatatt ttcgactata taagatacaa
     3961 ttattcacca tgttttatca agatatgcca taattgtatt a
//
LOCUS       DACTGW010000272         3985 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.275, whole genome shotgun sequence.
ACCESSION   DACTGW010000272 DACTGW010000000
VERSION     DACTGW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.275"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            172..366
                     /locus_tag="I9573_003353"
     CDS             172..366
                     /locus_tag="I9573_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HAT4797941.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIREFQDTIIL"
     gene            complement(468..977)
                     /gene="vanZ1"
                     /locus_tag="I9573_003354"
     CDS             complement(468..977)
                     /gene="vanZ1"
                     /locus_tag="I9573_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HAT4797942.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1297..1687)
                     /locus_tag="I9573_003355"
                     /pseudo
     CDS             complement(1297..1687)
                     /locus_tag="I9573_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            2131..2700
                     /locus_tag="I9573_003356"
     CDS             2131..2700
                     /locus_tag="I9573_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797943.1"
                     /translation="MKLRNPKIKKLLSVALLAALIGIPIGQTANAMEISNNVPSYIEN
                     NPTTIEDLEINDSQLPKPGTVTYIDINKLIKNLKENGIDTTLIENELYYRKAGTNKIV
                     WTKTGFDLYLSKGVALTLAGAGTGVAGLLAGAIPGIGQYLAAAIVGAITMHLGSNITS
                     GKIFKFKKMRYNKGGAYVTKYYLQSVRNQ"
     gene            2992..3309
                     /locus_tag="I9573_003357"
     CDS             2992..3309
                     /locus_tag="I9573_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797944.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYISIGLFTRRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
BASE COUNT         1541 a          492 c          468 g         1484 t
ORIGIN      
        1 caataatata cttttttcta taaatatttt acccataacc tttatttttt tattaaaatg
       61 cattataata aaaagtaaat ctaaatttga tacagtttta acaatcatta ttttcagaaa
      121 agtgatacta ttgaatatat taagaataat tagagatgga ggaactaaga tatgagcgta
      181 attgtaatgt gtagtcgttg tggaaaagaa atagataaat atagaaataa atatataact
      241 tattatgaga aaaataccaa tgcagaaatt aaagtttgta taaattgtgc attagattta
      301 ttagaaaaaa caagagaaaa tcaagaattg aacatacgag agtttcaaga tacaataatt
      361 ctttaataat ttaggaaagc acctatatag gtgcttttta tgtgtaaata attttatatg
      421 taactgtatc ttacgttcta ttttaagcta aacctatata cttatttcta taagtttgca
      481 aaaataacta atcctaaaaa taatacaaca ataattgtca caatcaatgc caatacattg
      541 ataattttta tggtattatt cttaaatatt ttcgataata gcataaaaac agctattcct
      601 atgattcctc ctagtgtgtt tcctatcaaa tcggttatat cacttgcacc tagtgcaaat
      661 atatactgaa gtgtttcaaa tgctaaacta gttataaaaa taggtataac tttttttata
      721 aagctccatt cttcttttag catacacaca tatatcccaa atggtacaaa tatagccaca
      781 tttaatataa tctctgatat atctactcta ttattaatta taagtgaccc agcaaaaggt
      841 actaagttta tgcttctaag attcatggtt tcaagagatg agatatcaaa ttgcaactta
      901 aatagtatta tccatgttaa aataattatg taaactataa ataatccttt tgtaatatta
      961 tgttttctag atttcattta ttacctcctt actatattat tgtattattt tattaataca
     1021 ataatatagt aaggtaattt atctgtcaac tatttcttct aaaattatat tccttttata
     1081 atttcaatat ctttatcata tttacatgat ttaagctatt gtagtatttc atatatcttt
     1141 aaattttaat tggtattatt ctctatattt tttactatca aacactaagg aaataatcac
     1201 ttttctttat ataatgtttt accatcacat aaaactaaca ttaaatccaa acgctactct
     1261 caattgtctc aaactaaaaa gatactgata gctttattag tcatcaatat cttcttcttt
     1321 caaattttta aagtattctt ctaatttgtc caagctctta ctatctaaaa cttcatcatc
     1381 atgtagtgct gaaataaaac tcttaaatga attgttatgc ataaaactaa aaaaactctt
     1441 tgtctcaact tttaagtaat ctttctcttt tatagctata gtataatgcg tacatctatc
     1501 taccttaata agtagttaaa aatcccttat ctactaaagt ttttaaaaat gtcaatgttg
     1561 tgctcttttt ccaatcgtat ttcaacttca ttgcttctat gatatctctt gaggctaata
     1621 catcatctgc tccccaaata tacttcatta gttttagttc tgcatgtggt aatttattaa
     1681 tcttcattag tttacacctc ttaccaattt atgtataatt aaattttata ctaataatag
     1741 tatgtaatca atcagattgg aacaaatata atattccaat ttattccata taaaaataat
     1801 taataaaaaa gaagtactct actttagaga gtacttttgg attatatatt caaaaattta
     1861 tctaacatga ttagagtatg ttttatgctt tagtgtgata attttagttc gttttattat
     1921 tataatatgt attatatttc attataatct ataagcttaa atacaggata tttatttcat
     1981 atacttaatt tatattaagt tttattatat gtattcttaa gttttactat attttttatt
     2041 aatatttaat gtagttaatt cttaagaatt atattctaca ataataatat atatctttga
     2101 tatataacta atttaaaatg gaggtatttt atgaaactaa gaaatccaaa gattaagaaa
     2161 ttgttatcag tagcattatt agcagctctt ataggtatac ctattggaca aacagccaat
     2221 gcaatggaaa tttcaaataa cgtcccaagt tatatagaaa acaatccaac aacaatagaa
     2281 gacttggaaa tcaatgactc tcaattacct aaaccaggta cagttacata catagatatt
     2341 aataagctaa ttaaaaacct taaggaaaat ggtatcgaca ctactcttat tgaaaatgaa
     2401 ttatattata gaaaagctgg aacaaataaa atagtttgga ctaagacagg ctttgattta
     2461 tatctttcta aaggagttgc attaacttta gctggtgctg gtacaggggt tgcagggctt
     2521 ttggctggtg ccattcctgg tataggacag tatcttgcag ctgctatagt tggagcaatt
     2581 acaatgcatc ttggttcaaa tataacatct ggaaaaatct ttaaatttaa aaaaatgcgt
     2641 tataataaag gtggggctta tgtaaccaaa tattacttac aatctgtaag aaatcaatag
     2701 gtgataaaat gattataaaa tatctacttt ttgcaattat ctcagcacta gttttattct
     2761 tttgtaataa agtatttcaa ctaagtaaat tacacaagtt cattatattg tttgtttcat
     2821 tttttatact aacttttgta gcatcattta ttaatagata aaaaataaga tggtggggtt
     2881 aacctaccat ctttttctca tttaatattt gtaatatttt agatactctt tatacttgtg
     2941 ttactgatat actttgatta agaaaagtaa atttaacagg aggtatctgc tatggatttt
     3001 ggaaatatca atcttatact tattggaata atagttataa taggaactac aataatatat
     3061 ttaattaaac ctaaaactgc tttttgtagt aaaaaatatt ttaataaatt agaatcaata
     3121 tatggaaata tagataagaa aaaaactgtt aaactagaag tactttatcg ctatgtaaca
     3181 ggtcttgagt atatatcaat aggcttattt acaagaagat tagatattac aataatagca
     3241 ataatattag tagctactat aacagtaata ttatattact tagttagaaa gagatatata
     3301 actatataaa atctaataga attaatatca aaagaaagta gcaactaatc accagtaatg
     3361 taaaataatt tgtttttcaa agttaataaa agtcttcttt atggcaatga tttaaatata
     3421 atcaaatatc gtaacggaga tttttttatg aataatttaa gaaaacaatt aatcaaagaa
     3481 attatataag atggagaact tataaaatct acagatataa gtagttttag gagtaaatac
     3541 agatgactta tttcaaagag acaatagcta caagttatcc tcattctgta atacaaaaat
     3601 gtttaataca ttagataaga aatactttaa atatgtttca tacaaggact caaaagaatt
     3661 aataagtgat tttaaatctg tatataggtc agttaatgaa taaaaagcct tatcgacttt
     3721 agataaaata gattaaaaat aatgtaaaca gtatacttca gaaattaaaa cgtttggaaa
     3781 taactggaat gttgtagcat cagcctttag ttttactaaa ataagtaata tctataatat
     3841 atattacaag tataatcgaa ggatttaata aatatttcaa aaatgtctca aaaacaaagt
     3901 ccagatttac atcaaacgct tcactcttaa agatattatt cttgacaagc gaaaatgtga
     3961 tgaaaaaatg gactatgaaa tataa
//
LOCUS       DACTGW010000273         3967 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.276, whole genome shotgun sequence.
ACCESSION   DACTGW010000273 DACTGW010000000
VERSION     DACTGW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.276"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            224..1714
                     /locus_tag="I9573_003358"
     CDS             224..1714
                     /locus_tag="I9573_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAT4797945.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1972..3468
                     /locus_tag="I9573_003359"
     CDS             1972..3468
                     /locus_tag="I9573_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAT4797946.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACISACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFEFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3602..3952
                     /locus_tag="I9573_003360"
     CDS             3602..3952
                     /locus_tag="I9573_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT4797947.1"
                     /translation="MKKIVLKDILFLVTTLSIIVILLFVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKNTAFKTTILSTGKSDCILIEIGDKFIMIDTGEDKNGEQIVDKL
                     KEKEINALDYLIKK"
BASE COUNT         1585 a          398 c          768 g         1216 t
ORIGIN      
        1 attaaaatat ataataaaaa atattatata ttagattaaa aagtaaaaat aaaaatagtg
       61 aaatttcaga aatatcaata atattatata ataaaataaa aaaacactat atatattcga
      121 taaaataaaa tttaaacata taatatttta tgtatatatt aataaaagga catattgtgt
      181 tataatggta tcgtataaaa tcatttacgg gggtggtatt aaaatgtttc aatttgaaac
      241 acaaatacac aaattaagac atgatgtttt aactgcaata gctagacttg gaaaagaaga
      301 taaccttacc agagaagcaa tggataatat tcagtatgaa ataataaaag gtgagaaacc
      361 tacatacaga tgttgtgttt ataaagaaag agctataata tctgagagag taaatgtaag
      421 tatggggtta caaccaggtc aagataagat agatttagat acaatggata tagaagaaga
      481 tgaacaaatt atatatattt tggaagctgc atgtgacagt tgtccaataa atgaatttac
      541 agtaacagat atttgtagag gatgtctagc acatagatgt aaagaagctt gtaaatttgg
      601 agctataaca catgtcggag gaatggccta tataaatcat gaattatgta aggcttgtgg
      661 aatgtgtaaa aaagcttgtc aatatgatgc catctcagag gttgttagac catgtaagag
      721 tgtttgtcca actaatgcct taggatttga tagagaaaat atgaaagcta tgatacatga
      781 ggataaatgt ttaaactgtg gagcatgtat gtcagcatgt ccatttggag caatatctga
      841 taagagtttg atagctcctg tagctagaaa gttggttcaa aaagagaaaa tgtatgcggt
      901 agtagctcca gctataacag gtcaagttga agcaaatgtt acatatggtc aaataaaaaa
      961 tgctataaaa tcactaggat ttgttgatgt attagaagca gcttgtggag cagatgcagt
     1021 tacagttcat gaaagcttag aatttgctga aagaatggaa aagggagagg cttttatgac
     1081 aaattcttgt tgtccaggat ttgttggtta tatagaaaaa acatacccaa atcatgtagg
     1141 aaaaatatct tctacagtgt ctcctatgat agctagtggt agatttataa aaagtcatga
     1201 accagatgct aaagttgtat tcataggacc atgtacagcc aaaaagagtg aggcagctaa
     1261 gaaagaatta aaaggagctg ttgattatgt acttactttt gaagaaataa tggcattatt
     1321 tgaggcattt gatatagact tggctaagtg ttcaaatgaa gatatagatg atgcatcaat
     1381 ttttggaaga ggttttggag ctgctggtgg tcttacaaaa gctatagaaa attatcttgc
     1441 tgaaaaaggt attggtgtta attttgaacc tgtaaagatt tctggttcaa gagaaatcaa
     1501 gaaaactatg actatggcta atttaggaaa gctagaaggt aattttatag aaggaatgat
     1561 gtgtgaaggt ggttgtataa atggttctgg taaattctta tcaggtgcaa aggttaaagc
     1621 cacttttgat agaaaaaatt ctcaatcaac taagaaaagt gtcttagcaa atacaaagat
     1681 aaaagatttt agtaatataa atttagaaaa ataaagataa tacaaagcaa caaaaataca
     1741 tattgttaat agagttgttc taagttgagg tttaactttt ggacaactct atttttattt
     1801 atatatttaa ttaatgaaca actaaaataa aaattacata tataatattt tgtatataac
     1861 ttaacaaagt atatgcctat gttataataa tttatagaaa taaataaaaa atacaatata
     1921 cataatagta gaaatatagt aaaaaggtgt gatttatagg gaggattact tatgtttgaa
     1981 tttgaaacgc aactaaaaag gttgaaacat gaagttttaa cagcagtagc aagactatct
     2041 attgaagaaa atttaacaaa agaaaatata gaaaaaattc catatgaaat aattaaagga
     2101 gaaaaggcga gatatagatg ttgtgtttat aaagagagag aagtagttct tgaaagggcg
     2161 caacttgcat taagtttaac tccaaatagt aaatatggag atataaatcc agattcagtt
     2221 agtttggaca cagatgagca aattatatat atagttgaag cagcatgtga tagatgtcca
     2281 ataaatgaat ttacagttac agagctttgt agagggtgtt tggctcatag atgtaaggaa
     2341 gcatgtaaat ttggggcaat ttcttatata aatgggagag catatataaa tcatgattta
     2401 tgtaaggctt gtggaatgtg caaaagttca tgtcaatatg atgctatatc agaagttgta
     2461 agaccttgta aaagcgtttg tccaacagga gctttggatt ttaatagaaa cactatgaaa
     2521 gcaatgatac atgaagataa ttgtataaat tgtggagcat gtatatcagc atgtcctttt
     2581 ggagcaatat ctgataagag tttgatagca cctgttgcaa aaaaacttgc aaagaaagaa
     2641 aatatgtatg caatagtagc tccagctata acaggtcaaa tagatagtaa tattacatat
     2701 ggtcagttaa aaaatgctat aaaatcactt ggatttgtag atatgataga agcttcttgt
     2761 ggagcagatg cagttacagt tcatgaaagc aatgagctta ttgaaagaat aaaatcagga
     2821 gataattaca tgacaaattc atgttgtccg ggatttttta agtatataga gaagatgttc
     2881 ccagatgaaa aagatagaat atcatctaca gtgtctccta tggtagcaac aggaagatta
     2941 gtaaaaaaaa tagattctga tgctaaagtt gtatttatag ggccatgtac agctaaaaag
     3001 agtgaggcaa cagagaaaag tttaaaagat tcagtagatt atgtacttac ttttgaagaa
     3061 ctaatggcat tatttgaagc atttaatata gatttatcaa aatgttcaaa tgaagatata
     3121 aatgatggtt cgatttttgg aaggggattt ggagcgtctg gtggacttgc caaatcagta
     3181 cagaactata ttgctgaaaa gggacttagt tttgagtttg aacctataaa agtatctggt
     3241 ccaagacaaa ttaaaaagac aatgacaatg gctaaattgg gaagattaga aggtaatttt
     3301 atagaaggaa tgatgtgtga agaaggttgt attaatggag caggtaagtt tttatcaggt
     3361 gtaaaagcta aaaatacttt tgatagaaaa aatggacaat ctactaaaaa gagtgtttta
     3421 tctaatgata agattaaaga atttgaagat gtaaatttag aaagataatt gttttggtac
     3481 agtcattttt ttatcatgcc acaaatatgc ctaaaatttt ccctttttat atgtgtattt
     3541 tacagagtta aggttgctat actataaata tgtaataaat aaaatttata ggagcttttg
     3601 gatgaagaaa atagtattaa aagatatcct gtttttagtt actacattat ctataatagt
     3661 aatattattg tttgtaacaa atataactac taatagtaag ataaaatcaa caagtgaatc
     3721 ttatttaaaa agtgacaaaa atagtgtgag gcaaaatggt atccagagga gcacaaaaaa
     3781 tactgcattt aagactacta tattaagtac tggaaagtct gactgtatat taatcgagat
     3841 aggtgataaa ttcataatga ttgatacagg agaagataaa aatggagaac aaatagttga
     3901 taaattaaaa gaaaaagaaa taaatgcact tgactatttg attaaaaaat agctacgcac
     3961 actcgtg
//
LOCUS       DACTGW010000274         3965 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.277, whole genome shotgun sequence.
ACCESSION   DACTGW010000274 DACTGW010000000
VERSION     DACTGW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.277"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1527
                     /locus_tag="I9573_003361"
     CDS             <1..1527
                     /locus_tag="I9573_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAT4797948.1"
                     /translation="LPIALISAAGIFLGISSAFSNPNIVSDIPFLENNFIQGILGFTK
                     AITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVLHTSVNYMLGCRGILAEP
                     DMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNIKLPDYLGFFGGTRFVPI
                     ITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFVFGFLERLLIPFGLHHIL
                     NAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFSTEATRFLAQGKIPIMVFG
                     LPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLEFSFLFVSPILFVIHAFL
                     SGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSIILGLVYALVYYNVFKVL
                     ILRFNLQTPGREEITDINFDLEPLSNKVEIAQSGETTKRALSIIKYLGGKENIEDITN
                     CITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIYGPSVSQVRDEVSFAMSI
                     "
     gene            1686..2438
                     /locus_tag="I9573_003362"
     CDS             1686..2438
                     /locus_tag="I9573_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAT4797949.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            2577..3917
                     /locus_tag="I9573_003363"
     CDS             2577..3917
                     /locus_tag="I9573_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HAT4797950.1"
                     /translation="MKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVVEE
                     RQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKIPL
                     QFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALKRV
                     FPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDLVP
                     KIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKVAK
                     SNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAHNK
                     HQIAIVITENNGAINNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAYEK
                     LAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
BASE COUNT         1478 a          458 c          696 g         1333 t
ORIGIN      
        1 ttgccaatag cattaatatc tgctgcaggt atatttttag gaataagttc agcattttca
       61 aatcctaata tagttagtga tatacctttt ttagaaaata attttattca aggaatattg
      121 ggttttacaa aagctataac aggagtactt tttggaaact tagcaatatt ctttgctata
      181 tcaatatctg ttggtcttgc taaagaagaa aaagcagttt ctgcattttc tggttttgtt
      241 ggttatttag tattacacac tagtgtaaat tatatgttag ggtgtagagg tattcttgct
      301 gaacctgaca tgatgagaga aatgggtcaa gctgtaactt taggcataca aacattagag
      361 ttaggagtat ttggtggaat tgtagttggt ataataacag catcattaca taataaatac
      421 tataatataa aactgccaga ttatttaggg ttctttggtg gaactaggtt tgttccaata
      481 ataacaacag tagtattctc tattttgggg ataataatac catatatatg gccatatgta
      541 aatacaatta ttcagagtgt aggtattggt atagctagtt taggatattt aggaaccttt
      601 gtatttggat ttcttgaaag gcttttaata ccatttggat tacatcatat attaaatgct
      661 atgtttagat ttactgaagt aggaggttct ttagttgtag gaggacaaga agtatttgga
      721 gcattgaaca tatttttagc tcaactaagt gaccctgaaa caatcagttt ttcaacagaa
      781 gcaactagat ttttagctca agggaaaata cctataatgg tatttggtct tcctgctgca
      841 gcatatgcca tgtataaaac tgcaaagcct gaaaataggt cgaaggttaa aggtatatta
      901 ctagctggtg ctttagcaac atttgtaaca ggaattacgg aaccattgga attttcattc
      961 ttatttgtat caccaatatt atttgttata catgcatttt tatcaggtat atcatttatg
     1021 atgatgcata tatttggagt agcaatagga aatactcaag gtggcataat agatttagta
     1081 gtatttggag ttatgcaacc aaatacaaag tggatttatt ctataatact tggtttagtg
     1141 tatgcactgg tttattataa tgtatttaag gttttgatac ttagatttaa cttacaaact
     1201 ccaggacgag aagaaataac agatataaat tttgatttag aacctttatc aaataaagta
     1261 gaaatagctc aaagtggaga aactactaag agagcattat ccataattaa atatttaggt
     1321 ggtaaggaga atatagaaga tataacaaac tgtataactc gcttaagagt agtagttaaa
     1381 gatatgagta aagtagatga aaatgctttt aaaaaatata caggtgcaat gggcgttata
     1441 aaagtatctg ataaagatat ccaaataatt tatgggccat ctgtatctca agtaagagac
     1501 gaagtttctt ttgcaatgtc tatttaatgt ctatctaatg taaatattaa aaggcaatac
     1561 taatttataa tttgtattgt cttttttgta gtataataaa cataagataa atgttgtata
     1621 gtgtatattt atataataaa tatgagaaag ttgtttaact acaaatcatt attatggagg
     1681 tataaatgaa cctaatagac aaattagata taaatcaaaa aaaattatct acaaatgaaa
     1741 tatctctttt aaactatttt ctaaaaaatt ctgaaaaaat aaatcaaaaa aagattaaag
     1801 atatagcaaa tgaaactttt aattcgactg catacatagt aaggttttgt cagaagctcg
     1861 gcttttctgg atatagtgaa tttaaaaact ttttagtttt ttcatattca aatagaaatg
     1921 aaagaaataa ggagtttact aaaagtcaaa gtgatatttt tactgatgtt atggatacac
     1981 aaaatttaat aaatgtagag aatatagata tgtgtttaaa tgaaatacat aattctaagc
     2041 atatatattt ttttggtgtt gggtcttcta gattagtatg tactgaaatg tctcaacgtt
     2101 tttcagctat aggtgtaaat gtaaaatact atgatgattc aactctcatg tatttggctg
     2161 catcaaatat tactaaagat gaccttgtaa ttgctgtatc tatgtctgga gagactgctc
     2221 aagttattaa ggcatgtaat atagcaaaaa caaatcaagt taaagtaatt tctctgacaa
     2281 atataagtgt caattcatta tcaaatatag ctgatataaa attatttgta tcttctccac
     2341 aatataattt taatagtgtt aactatacgt ctagagtgcc agcactttct cttatggaat
     2401 atatgtttca taaatacatt gaaaaataca atagatgaaa aaaattacaa ttttgaaaaa
     2461 aatactaaaa ttgaactatt tttaatgatt acctaaaagt attcatgtag aaaactttat
     2521 atattataat catcttacaa gttagttaat aaaaaacagg ggtgattaat atgacaatga
     2581 aaaaaagata caatatagct atagttggag gaggcagtac ttggactcca ggacttttaa
     2641 agtctttatg taaaatgagt gatagatttc ctctaaataa agtaactatg ttagatgtag
     2701 tagaagaacg tcaaagatta ataggggaat ttggaaaaat attatttaaa gaagaatatc
     2761 ctaaagcaac acttgaatat actacaaacc cagatgaagc atttatagat gtagattttg
     2821 tatttgttca aatgagaact ggtggtctta agatgcgaga attagatgaa aagattcctc
     2881 tacaatttgg actggttggt caagaaactt gtggagctgg tggatttgct tatgggttaa
     2941 ggtctatagg tgatatgata gaaatggtta agactgtaag aaaatactct cctaaagcat
     3001 ggatattaaa ttatacaaat cctgctgcta ttgttgcaga agcattaaaa agagtatttc
     3061 cagatgataa aagattactt aatatgtgtg accaaccagt taacttgtta aggtcttatg
     3121 gaagattgtt agacatggat tcaagaacat ttgagcctgt atactttgga ttaaatcact
     3181 ttggatggtt tacacatttg tatgataaaa atggtgaaga tttggtacct aagataaaag
     3241 atttagtagc aaactctgga ttccaacctg ttgatgcaga acaaagagat aaatcttggt
     3301 tagacactta tggaatggtt aaagatatgt tggaagatgt accagattac ttacctaata
     3361 catatgtaca gtattatcta tatcctgata aaaaagtagc taaatcaaat ataaattgta
     3421 ctagagcaag agaagttatg aatggaagag aaaagcgtgt atttgaagaa tgtaaatctg
     3481 ttatagaaaa aggaacatct ataggttcta atttagtgca taatgatgct catggggaat
     3541 ttatagttga agttgcagaa gcaatagctc ataacaaaca ccaaatagcc atagttataa
     3601 cagaaaataa tggggctatt aacaacttac ctgatgatgc aatggtagaa gtagctgcga
     3661 ttcttacaaa gaatggaccg agacctttac atgtaggtaa tataccaaca ttttataagg
     3721 gactgttaga aggtcaacta gcttatgaaa agttagcagt tgatgcatac tttgaacaat
     3781 cttatgaaaa agctatttta tctttaacat taaatagaac tataatctct ccaactaagg
     3841 caagacaagt tttagatgct ttaattaaag tgaataaaga ttactggcca acactatata
     3901 aagaaaatga aaaatagtca ggtctaagtc ttaattaata tattaattaa gaaatgtccc
     3961 ctttt
//
LOCUS       DACTGW010000275         3939 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.278, whole genome shotgun sequence.
ACCESSION   DACTGW010000275 DACTGW010000000
VERSION     DACTGW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.278"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..239
                     /locus_tag="I9573_003364"
     CDS             <1..239
                     /locus_tag="I9573_003364"
                     /inference="COORDINATES: protein motif:HMM:NF014225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797951.1"
                     /translation="VEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            497..1546
                     /gene="carA"
                     /locus_tag="I9573_003365"
     CDS             497..1546
                     /gene="carA"
                     /locus_tag="I9573_003365"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAT4797952.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1739..>3939
                     /gene="carB"
                     /locus_tag="I9573_003366"
     CDS             1739..>3939
                     /gene="carB"
                     /locus_tag="I9573_003366"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HAT4797953.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYL"
BASE COUNT         1630 a          400 c          777 g         1132 t
ORIGIN      
        1 gagtagaaga atctagacca aacatattag atgctataag aaataaacaa gttgatatag
       61 taataaatac accaactaaa ggaaatgact ctacaagaga tggatttaag ataagaagaa
      121 cagctattga attttctact gaaataatga catctttgga tacactaaaa gctttagtag
      181 aagttaagaa gaaacattta aataaagatg agttaaaagt ttacaatata gctgaataga
      241 aattaagttg attgtatagt ttaagaattt aattattaaa taaaaaaaaa gaggttattt
      301 tttctaggag tttttacttt tgagataact tctttttttt aagaaatttg tagtataata
      361 gaaaaagata aaaaataaat gtaaataagt ataaaactag aatgtaaaag tgaaaaatag
      421 atatagatat ttaaaagctt atatttagtt aatgttcttt agtttgtcta aagagaatta
      481 taggaggtac tgtagtatga aagcaaggtt aatattagaa gatggaacag tttttatagg
      541 aaaggcattt ggatatttag aagaaagtgt aggagaagta gtatttaata cttcgatgat
      601 aggatatggt gaagttttaa cagacccttc ttactatggt caaatagtta ctatgactta
      661 tcctcttgta ggaaattatg gtataaattt atcatcagca gagtctgaaa aagttcaagt
      721 aaagggattc atagtaaggg agaaaagtga ttctccaagt aattttagat gtgaaattga
      781 tatagaccaa tatttaaagc aaaataaagt tatcggactt gaaggaatag atacaagagc
      841 tttgactaag atacttagaa ataatggaac aatgaaaggt ataattacat tagaagacag
      901 taaattagaa gatgttaaac ataaattgga taagttttca aatacagaag cagttagaac
      961 tgttacaaga aaagaagtag agcatataaa aggaaatggt ccaaaagtgg ctgttatgga
     1021 ttttggagta aaaagaaata tattaagatc atttatagct cgtggatgtg atataacaat
     1081 atttccagct acaacatctc cagaagatgt attgagtata aaccctgact taatattctt
     1141 gtcaaatgga cctggagacc cagaagacct agaagatgtt atagaaaata taaaagctct
     1201 tattggtaaa aaacctatag ttggaatatg cttaggacat caacttttag ctttagctct
     1261 tggaggaaaa actgctaaac ttaaatttgg gcatagaggt ggaaatcatc cagttaaaga
     1321 tttagaagaa ggaaaagtat ttataacttc tcaaaatcat ggttattatg tttcagaagt
     1381 tcctgaacaa atgaaagtga cacatataaa tttaaatgat aatactgttg aaggaatgag
     1441 acatgagaag ctagatgtat acagtgttca ataccatcct gaagcttgcc caggaccaaa
     1501 agataatgac tatatttttg ataaattttt agaattagta aaataaataa cattagaata
     1561 gctaactaaa aaacaagaat aacttaaatc aaaaagtttg gtttaattaa catagatgta
     1621 ttaaatagat gtattgatga gtaaatttga acttaagata aaataaagta attaaagttg
     1681 attgtataga tgatgaagat aagaactgtg aaaattgttt agataaggag aaagagttat
     1741 gcctaaatta gatagtataa agaaaacttt agtattaggg tcagggccaa taataatagg
     1801 tcaagctgca gagtttgact attcaggaac acaggcctgc caagcattaa aagaagaggg
     1861 aattgaagtt gtattagtaa atagtaaccc agctactata atgactgata aagaaattgc
     1921 agataaaata tatatagaac cattaacaat agaatttata gaaaaaataa tagaaaaaga
     1981 aagaccagac agtttacttg caggtatggg tggtcagaca ggattaaact tagctgtaga
     2041 attacatgat gctggtatat tagataaata taatgtaaaa gttataggaa catctataga
     2101 atctataaaa aagggcgaag atagagattt atttagagaa gtaatgaaag aaataaatca
     2161 accagtaata gttagtgata tagttacaaa tttagaagca ggtcttgagt ttgccaataa
     2221 gataggatat ccagtagttg taagaccagc atatacgcta ggtggaactg gtggaggtat
     2281 agctgatact gaggaagaat taagagaaat actttcacat ggactacagc taagtcctgt
     2341 tggacaagtt cttcttgaaa agagtataaa aggttggaaa gaaatagaat atgaagtaat
     2401 gagagatgga aatggaaact gtataactgt atgtaatatg gaaaatgtag acccagttgg
     2461 agttcataca ggagacagta tagttgttgc tccaagtcaa actctaagtg atgaagagta
     2521 tcaactactt agaaaggcat caatagacat aataaatgca atagaggttc aaggtggatg
     2581 taacgttcaa atagctttaa acccacatag tttagaatat gcgataatag aaataaaccc
     2641 aagagttagt aggtcatcag ccctagcatc aaaagctaca ggatacccaa tagcaaaagt
     2701 agcagctaag atagctttag gttatacttt ggatgagata gaaaatgcag taactaaaaa
     2761 aacttatgct tgttttgagc caacattaga ttatgtggta gtaaaaatac caaagtggcc
     2821 atttgataaa tttagacatg caaatagaaa attaggaact aagatgatgg caactggtga
     2881 aatcatgagt ataggaagta actttgaggc tgctattctt aaagggataa gatcacttga
     2941 aacaggaaaa tattcattgg ttcatgctcc atcagaaggt agaacattag aagaattaaa
     3001 ggctagagta gtagtacctg atgatgagag actatttgac ttagctgaaa tgataagaag
     3061 aggctataaa gtagaaatga tatcagaaat aactggtgtt gataagtggt ttataaataa
     3121 atttaaatgg atagtagaac aagaagaaaa gttaaaagtt ctaaaaatag aagacttaga
     3181 taaagaatat ttacatgaat taaagaaaaa gggattttct gataaaggta tatctgactt
     3241 aatgaagata agcccagaaa aattatatga attgagaagt ttatataata tacaaccagt
     3301 ttataaaatg gttgatacat gtggaggaga atttgaagct ctatcacctt attactattc
     3361 aacttatgaa caatatgatg aggtagtagt tagtgataag agaaaagttg tagtattggg
     3421 ttctggtcca ataagaatag gtcaaggtat agaatttgac tattgttcag ttcactgtgt
     3481 aaaatcttta agaaaaatgg gaatagaaac tataattgta aacaataacc ctgaaactgt
     3541 aagtacggat tttgatacat ctgataagtt gtattttgaa ccattaacag aagaagaagt
     3601 tttaaatata atagaaaaag aaaaaccaga aggtgtaata ctacagtttg gtggacaaac
     3661 agctataaaa ttggctaagt ttttacatga aaaaaatata cctatactgg gaacagactt
     3721 tagagatata gatgcagcag aagatagaga aaaatttgat gatttactag agagactaga
     3781 tataaataga ccaaaaggaa aaggtgtatg gacaactaat gaaggtgttg aaattgctaa
     3841 ggagttaggt tatccagtat tagttagacc atcttatgta cttggtggtc aaggtatgga
     3901 aataacttat aacgaagaaa aactaattca gtatttgga
//
LOCUS       DACTGW010000276         3921 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.279, whole genome shotgun sequence.
ACCESSION   DACTGW010000276 DACTGW010000000
VERSION     DACTGW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.279"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            4..864
                     /locus_tag="I9573_003367"
     CDS             4..864
                     /locus_tag="I9573_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4797954.1"
                     /translation="MAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTTFMRI
                     LATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVLSDIP
                     LRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEPTAGL
                     DPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILDSGKVIYNGSTEALAKL
                     AEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPTIEDG
                     YMLIMGGDNI"
     gene            861..2150
                     /locus_tag="I9573_003368"
     CDS             861..2150
                     /locus_tag="I9573_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797955.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIDEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINLIWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFIMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2080..2724
                     /locus_tag="I9573_003369"
     CDS             2080..2724
                     /locus_tag="I9573_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4797956.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKSLSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPIKRKIR"
     gene            complement(2991..3455)
                     /locus_tag="I9573_003370"
     CDS             complement(2991..3455)
                     /locus_tag="I9573_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4797957.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLS
                     KEDKRKNFISFINDNKELIGFINLLDEGSYVFFGIGIKPDYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYKSQGFKIVETQVQKTHLGDGEFFVMRYQ"
BASE COUNT         1468 a          468 c          596 g         1389 t
ORIGIN      
        1 aaaatagcag tatccaatct tagtaaaaga tttgggaaaa aacaagctct tgataatata
       61 aatcttgaaa tcgacagtgg tatgtttggt cttttaggaa gaaatggagc aggaaaaacc
      121 acatttatga gaattttagc cactttatta tcaaaaacag aaggttcaat acatatgtgt
      181 ggagttccta ttgagaatac taaggaaatt agaaatatgg ttggttattt gccacaagat
      241 ttttcaatgt atcctaacat gacagtatat aaagcaatgg attatttagc agttttatcg
      301 gatataccac taagaaaaag aagaaaaatt atagcaaagt tattaagcaa agtaaacctt
      361 actaattatc aaaatgtgaa agtaaaatct ttatctggag gtatgaatag aaggcttggg
      421 atagcacaag cattaattca taatcccaga gttttaatag tggatgagcc gacagcaggt
      481 cttgacccag aagaacgtat aagatttaga aatttactat gcgaaattgc aaatgataga
      541 atcgttattt tatctacaca tattgttgga gatatagaag ctacatgtga aaacgtagct
      601 atattggata gtggaaaagt catatacaat ggctcaacag aagcattagc aaaactagct
      661 gaagggaaag tatatagtat tgaagtagat aaaaaagaca ttgaaaatat aaaaagtaga
      721 tttatagtta taggaatgtt gactcatggt ggaaaggcta tacttaggat tatatcagat
      781 gataaacctt ttgagacagc agtaaattgt aatccaacta ttgaagatgg ctatatgctt
      841 ataatgggag gagataatat atgaatctat ttataaaaga atgtaaaaat atattaaaga
      901 gtatggtata ttatattttt attgtagttt tagttttttt ctatatttct caacttggaa
      961 gttcagtttc aaatgatgta agtaaaaata ttcctaatga tggaaatcca ttagttgctc
     1021 cacaaaaagt agagaatatt gacgaatatt actctgtaca caatcaatat ccatatggtc
     1081 aaaaagaatc tatgaaaaca gaaaaggtca ttccaaatgc aactataaac ctaattagag
     1141 aatatcaaaa aaacacatat acaacgtatc caacaggggt agttaagtat ataaaattag
     1201 accaaaataa aacgaagacc atagaaaaat atatagaaga aataactggt aaaagtatta
     1261 gcgaaataaa tttaatttgg aaaactgatg aaagtataaa aatagattat tctaaagtat
     1321 ctcttgaaag atttgaagaa ataatgcaaa gagtagacga cattttagga ggagggtctg
     1381 catatgcttt tgacaagtta caaagatttg gagtagttcc agttacctat gatgaagcta
     1441 tgaagaatta caactataca atacaaaaag ataaagttag taatgggtat gcaagaattt
     1501 tttgcgatta tataggtata gttttaagta tatttccagt atttgtggca gtttttatgt
     1561 cacttaaaga taagcgttca agaatgagag agattattta ttcaaaacaa gtatcttcat
     1621 gcaaaatagt tttatcaaga tattttgcac ttgtctttat aatgatattg cctgtattaa
     1681 ttttaggtat gaaagattta cttcctttga ttttctttgc taaagataaa ggtatatcta
     1741 ttgataactt agcatttata aaatatacat tatggtggat actaccaact cttatgatag
     1801 ttacagctgt aggaatgagt attactatat taacagatac accagtagca attattgtgc
     1861 aattaatttg gtggctttac agtataaatt ctacaggact caaaggtgat tattcaacaa
     1921 tggggctaat gataagacat aatagtgtat taaatgggga tattattata caaaacttca
     1981 atcaaattat gattaataga attgtgataa gcttgggagc attggtgctt gttttgttag
     2041 ctatattctt atatgagcaa aaaagaaggg ggaatcttga tgtttgggca aagttttcaa
     2101 aactgtttag ttctaataaa agaaaacttc aaattaagca ctttaactaa tttaatatgt
     2161 tcaattatat tgctatgtat gattcctcta atacaaggag tagctaattt agacagaatt
     2221 tcaacagcta tatgtttaga aaattatgtt gcaataatag ggatagtaat gttggtacct
     2281 gtattttcgc tagagcaatc aaaagaaatt gatgagatag tatcttcaaa atcattatct
     2341 caagttattg tacaattaat taggatactg atgtcaatta taggtatagt ggtattaatc
     2401 ataggttttg taaatgtttt aatatatctt ggatgtaatt ttccagtagt tgagtatatt
     2461 attggaagtt tcgcatcagc tatgtttata ggaagtttag gaatgttttt ctcaaattta
     2521 tttaataata caatcatagg ttacatgata tctataggat atttaatttt aaatatgatg
     2581 actaaaggca agtatgttgg aaaatttttt ataatgtcta tgagtcaagg tagttttgat
     2641 gagaaatatt ggctgatact tggaagtgtt gtgttgataa ttgcatctct cttaataaaa
     2701 ccaataaaaa ggaagattag gtaataaaat gagagcttag ataaaataaa ggtaatacaa
     2761 ataaaaagta catttatgaa atataaattt atttttatat acacaaacct acttacaatc
     2821 tctttatgta tgtagataga tttatatatc tgtagtataa aatatatatg tataccgaat
     2881 tttatcaaag gctctcaaat ttatatttca aagacactta ttgctatgac atacacacat
     2941 gtattttttc attttaattg tcatattagc aatatgacta gtgttataaa ctattgatac
     3001 ctcataacaa aaaattctcc atcccctaaa tgcgttttct gtacctgagt ctcaactatt
     3061 ttaaatcctt gagacttata acattttaca gctctcatgt tccaggttct tacatttaat
     3121 acaattggtt tagtactata tttatttcta cattcttgta atcctaggct aattatttct
     3181 ttgccaattc ccattccaca ataatctggt tttataccaa taccaaagaa tacataactt
     3241 ccttcatcca ataagtttat aaaacctatc aattctttat tgtcatttat aaaacttata
     3301 aaattttttc ttttatcttc ttttgatagt gaaaaattct tctttttaat ttcctcccat
     3361 ggaggtaggt tgtatattga atatttacct tcatatttcc aattagtaac ttcaatagca
     3421 tgttcttcag taaatttaac tttatttaaa ttcatatgtc ctcctattaa aatatttaag
     3481 gtaattaatg gtattttaaa ttttacaaaa taagtaaaac tatttatatc tatccataaa
     3541 atctttaaaa attttactaa caattcttgt accctgtact ttttattgga ttcacagaag
     3601 actataaact aattttgaat taccttctaa ttcaaaaaca tcacgatata tgtaatttct
     3661 tagcttgatt aaattctgtt aaatctgttt cttatagttg tataatcaac atcaatatct
     3721 ttatgtgtat tagttaattt attggtttta ggaacttatt attaatatat tgtatttgct
     3781 ccaatactcc aagtagtagg taaattatag gcaaattatc aaatgtatat ctatccatgc
     3841 ttaaacttta gagatacaat aactactaaa ttagtattct agtagttata aaaaagaatc
     3901 tttaaaaata ttaatttaac t
//
LOCUS       DACTGW010000277         3918 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.280, whole genome shotgun sequence.
ACCESSION   DACTGW010000277 DACTGW010000000
VERSION     DACTGW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.280"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            3..311
                     /locus_tag="I9573_003371"
     CDS             3..311
                     /locus_tag="I9573_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAT4797958.1"
                     /translation="MFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFIPVAIL
                     TSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYLFGIIL
                     "
     gene            complement(517..2577)
                     /locus_tag="I9573_003372"
     CDS             complement(517..2577)
                     /locus_tag="I9573_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAT4797959.1"
                     /translation="MEKYRALKLVSLIIISISFPLTILLATIGLAEPKYNIQKTSTKS
                     GIFNISNDFYDSNIFDEGTVESLLSNFKISVDSQLTSEEKSKLQTKSKSIDLSSNDSE
                     YDKIMINKQASEEYLSYLKNIKVYVINTYTGVFYTNTSYGNLDDFKQKTKSYCNIEII
                     NNVGKSSYIKTINGEKFELNNLSMELNGVDEAFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVACLSLAIVCLLSIILYSRIKGEVLKRNNVFLLIYSKIPMEFNVILVF
                     ISIAILTMYALAVSYHIDVIVLVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYIKVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(2773..3465)
                     /locus_tag="I9573_003373"
     CDS             complement(2773..3465)
                     /locus_tag="I9573_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4797960.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
BASE COUNT         1301 a          613 c          350 g         1654 t
ORIGIN      
        1 atatattttt tattataatt ggaatgtaca ttgttacata tctaccaaga gtgcttccta
       61 tgttaatttt ttcaaaaaaa gagatgcctg attttttgaa aaaaatcatg aaatttatac
      121 ctgtagcaat acttacttcc ttaactgcta aagatgtatt ttttaatgga gatagtctat
      181 atttatctat agctaaccct aaaattatat cttttatgat tgtacttctt gttgcatata
      241 agtttaaatc tattggtatt tctattatta caggagtgat atctatatat ttatttggaa
      301 ttatcttgtg atatttggat aatgttttat tgtataagtt attttaaaac tatgtgtaca
      361 attttaattt aagttgttat attattgatt ttaaataaaa aacagagtga cttaaagtta
      421 ctctgttttt actcttcatt ctctactgta aatattctaa tatattataa taaaagcttt
      481 cgacagtaaa aaaaatatat atacttaaat actatctcat tccatatttg ttatttcttc
      541 ttcatatttt tgaaactcta aatttgcttt aaataaatct ccatctattt ctatataaaa
      601 ttttccacct tgtaaagcta ctaaatctct tgcaatagca agccctaatc cacttccttc
      661 agtattcctt gattcatcag ctcttttaaa tctttccata atctcacttg ggtcaaaatt
      721 taattcataa gaagatatat ttttcatagt tacctttaca ttttcaccat agtcatatac
      781 atcaatatag actctagttt cttctaagga atattttgct atattagata aaagattttc
      841 aaatactcta tacattcttc ttccatctgc ccatatatac accttatcat ttggaacatt
      901 taattttaaa tataaattat ttttagataa tttttcttca agttcaccta tgctttgtct
      961 taatagttgc actaaatcaa tcttgctaat ttgtaattct atattgccac tacttactct
     1021 actcgcttca aataaatctt ctatcaataa ttttagtctt tttgactttg actcaagtac
     1081 ctttatataa tcatttatat actctggttg tattgattct tcttttttaa ttaaatctac
     1141 ataatttatt atagaagtaa gtggagtttt caaatcatga gatacatttg ttataagatc
     1201 agacttcatt ctttcacttt ttacttgctc ttctatagct ctatcaagac cttctccaat
     1261 attatttata ttttcagcta aagacgtaaa attatcattc ccagtaattt ttatcttata
     1321 atctaattct ccctttttta ttctttctgt accttctatt acgtaactaa gatatgaaag
     1381 tcttttagtt atatatattg taaatcctaa aaaaatacat atagaaaaca gtaatcccat
     1441 acctatactt cgagtgctaa ttaccatgat taaagctaaa gatattatca ctataacaat
     1501 aactttcctt gctaaagata ctttctttat agcgttactg acctccctta cagtttttat
     1561 taaccataaa atcactctta ctataatact tgtcttaaat atacacattt tattttctaa
     1621 ataactcaat tgcacattta taaaaaatat attatttgat actatcagta aagataaaga
     1681 aactaataca attacatcaa tatgataaga tacagcaaga gcatacatag ttaaaattgc
     1741 aattgaaata aatactagta ttacattaaa ttccataggt atcttactgt atattagtaa
     1801 aaatacatta tttcttttta aaacttcacc ttttattctg ctatataaaa tgatagataa
     1861 taaacacaca attgctaaac ttaaacaagc tacaaaaaat aatcttactt tttctgtagc
     1921 ttgtttaaat atttgaaaat tagtgtatac tattccatct tctatagttg gctcttctgg
     1981 aaaagagaca tatgcctcaa aggcttcatc tactccattt agttccatac tcagattatt
     2041 cagttcaaat ttttctccat ttattgtttt aatatacgag ctctttccta cattatttat
     2101 tatctctata ttacaatagc tcttagtttt ttgtttaaaa tcatctaaat taccatatga
     2161 tgtatttgta taaaatactc ctgtatatgt attaataaca tacactttta tattttttag
     2221 ataacttaaa tattcttcag aagcttgttt attaatcatg attttatcat actcagaatc
     2281 attactactt aaatctatac ttttactttt tgtttggagt ttactttttt cttcactagt
     2341 taactgacta tccacagata tcttaaaatt agataataag ctttccactg ttccttcatc
     2401 aaatatatta ctatcataaa aatcattaga tatattaaaa atcccactct ttgtagaagt
     2461 tttttgtata ttgtatttag gctcagcaag tcctattgta gccaacaata tagttaatgg
     2521 aaatgagata gatattatga ttaatgaaac taattttaat gctctatatt tttccaagtt
     2581 gcccacctca tctacattgt cagtatttta tataaaataa agtttaagaa aagcataatc
     2641 cccatagagt aaattaaact ctcagagaaa aaattaaaac atattaaaat tcctaacatt
     2701 tgtgtaaatg taaaaatgac taaaatcaaa atgcaattaa atattaaact cttttctttt
     2761 ataatttttc cactaaaatt tttcaatctt ataaccaact ccccacacca cctttaaaaa
     2821 tcttggttct tttggattta tttcaatctt ttctcttatt cgtctgatat gtactgctac
     2881 tgtattttct gaattaaaac tttcctcatt ccatactttt tcgtatatct catctattga
     2941 gaaaactcgt cctttatttt ctaaaagaag ttcaattatt ttatactcaa ttggggtcat
     3001 ttttatgttt tcaccatcaa ttttaacttc ttttgttagt gtatttaact ccaaagctcc
     3061 actttttaaa atttctttac atgtatcttc ataattacct aaagttacat atcgtcttaa
     3121 gtttgattta accctagcta taagctctaa tagattaaat ggctttgtta tataatcatc
     3181 agctccaata tttaatccta ttattttatc tgtatcttca ctttttgctg aaattaaaat
     3241 tattggaata tttctttctt ctcgtatttt tacagttgct ttgattccat ctaatttagg
     3301 catcattata tccattagta ttagatgaat gtcattctct gttattattt ccaaagcttt
     3361 cattccatca tatgctttat atattttaaa tccttctgat ttaagatata tttctattga
     3421 atcaacaatt tctttatcat cgtctactac taatatattg tacatatttt cacttcctct
     3481 attgtataat aatacttatc tatattatac aatatctttc acttactttc tcaacaatga
     3541 ctaaattgtt atattacttt ggcttacatt tttgtaactt tcccttataa ctatataata
     3601 aaatataaat cttaagaaaa tattatccta attcttaaat atttcttaag atttaagata
     3661 aattgagatt tatttgaata aattgatttg aattatgtta ttttcagatt acttttattt
     3721 aagtattttg attggattca gctgaattaa tgtatattaa ttcttattga acagttttta
     3781 tttttggggt ggaagaatgg tggaagaatt acatttcact aattttcttc caccattctt
     3841 ccacccaaat atacttagaa acatttattt cactgcaata atagctactt atttaaattc
     3901 tatttttata cattctat
//
LOCUS       DACTGW010000278         3882 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.281, whole genome shotgun sequence.
ACCESSION   DACTGW010000278 DACTGW010000000
VERSION     DACTGW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.281"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            257..1888
                     /locus_tag="I9573_003374"
     CDS             257..1888
                     /locus_tag="I9573_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HAT4797961.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(2023..2949)
                     /gene="fba"
                     /locus_tag="I9573_003375"
     CDS             complement(2023..2949)
                     /gene="fba"
                     /locus_tag="I9573_003375"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HAT4797962.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            3279..3608
                     /locus_tag="I9573_003376"
     CDS             3279..3608
                     /locus_tag="I9573_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HAT4797963.1"
                     /translation="MPQIKIRGINENDICKISKKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            3608..3811
                     /locus_tag="I9573_003377"
                     /pseudo
     CDS             3608..3811
                     /locus_tag="I9573_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
BASE COUNT         1555 a          470 c          651 g         1206 t
ORIGIN      
        1 cttaaattta attaaattgt attaaataga caaataatta ctaaatatgt gataaaatac
       61 aattaaaagc aaaatgaaat ttttaacaaa ctataatcat taaaattata tcttagttac
      121 gttttaatga aataaactac ttgaaaagac gaatattata aggagttgat tatatgtgct
      181 gatggtattt attgtaagca aacaataaat tttgtatgta gttcaataaa ttacttaatg
      241 aaaggtgcga atattaatgg ataataattt aatagggctt atagaacatg tagaaaatcc
      301 agccatttta tgtaaagaat caggcgaaat aatatactgt aatcatctaa tagatagtat
      361 ttttagtttt ctagatataa aaaagcctag aaatataaat gaattagatt caaattttga
      421 caaaacagaa atactgacag atagtaaaaa gaaaatagct ttcagagagt taaggatgac
      481 tgcacatata tacaatatga aagataataa taatgaaaat aatatagttt atttatttga
      541 gaagtctcta atatctgata aagtaattga agatataata gagcatatag atgaagttgt
      601 tgttgtgttt aacaaagatg gggtaattga aaagatgaac actgtcagtg atgagatatt
      661 accttttaaa agaactgagg tacttggtag aaatataaca gaccttgtga gacaaggatt
      721 agttgaagaa ccaataatat taaacatgct taaagtgaag aaaaagattt atagaaatat
      781 cgtttaccca gatgggaagc ttattgcata tacggcagtt ccaagatggg attctaaggg
      841 aaagcttaca ggaggagtac taacagggag agatatttca agggttatta agcttgaatc
      901 tcagataaaa tatagtgata tatcagaaga cacagagtat ataagtcaaa gtaagattat
      961 ggacaatata aaaaaagttg ttaagagagc tgcagcctca gattcttcta tatttataaa
     1021 tggagaatca ggtgtaggaa aagaaataat agcaagaaca atatataagt atagttcaag
     1081 gagagacaaa ccttttatag ctataaactg tggagctata ccaaatgaat tattagagtc
     1141 agagtttttt ggatatgaag aaggttcttt tactggagct aagaaaaaag gtaaaaaggg
     1201 actttttgaa gaggcgaatg gtggaactat tttcttggat gagataggtg agttaccaat
     1261 gcagatgcaa aaaaaattac ttagggttat acaagaaaat actattacta gaataggtgg
     1321 tagtaagcct ataaaaatag atgttagata tataagtgct actaatattt ctcatgaaga
     1381 ccttagaaat aaccttaaat ttagacaaga tttgtactat agattaagtg ttattccagt
     1441 aaaaatacct cctcttagag aaagaaaaga ggatatagta ccattagtaa attatttctt
     1501 aaaactttac aatgaaaagt acaatagaga agtagaagtt tcacctaaag ttattgagtt
     1561 attagaagaa tattcttggc caggaaacat aagagaatta aaaaacatca ttgaaagatt
     1621 tgtagtatta tcagctaaaa atgtaattgg agaagatgaa tttaacatgc tgataaattt
     1681 agatatgatt gataatgaaa cagatgattt atcaccaata gtggtaaatg gaattatgaa
     1741 tcttaatgat gcatataaaa tagtggacca aataatgata agtaaggcta taaataagta
     1801 tggttcaata acaaaggctg ctgaagttat aggtatatat ccttctacca tccatagaaa
     1861 gataaagagt ggacatattc acgtttagct gtcagtttaa aaagacaata taattaaaat
     1921 attataagat acataattga tacaacttaa aagagatggc ttaaaaataa atttaattac
     1981 tttaagtcat ctcttttctt ttataaatat ttaaaagtta tattagttta tagagttagc
     2041 agaacctaaa acatcatcta tcttgctttc aactacagct tgtatagcat ctctaccagc
     2101 accaatgtat tttcttgggt caaattcttt tggattttca gctaagaatt ttctgatagc
     2161 agcagtcata gctagtctta agtcagtatc catgtttatt ttacaaacag ccatagaaga
     2221 tgcttttctt aacatatcta caggtacacc tttagcacca gctatatttc caccaaattc
     2281 attacatgta gcaacagcgt tttggtcaac agcagatgca ccatgaagaa ctattgggaa
     2341 acctggtaat ttgctttgga tttcttctag tatatcaaat cttaatttag cttctccttt
     2401 gaatttgaaa gcaccatgag aagttccaat agctattgct aatgaatcaa cacctgttct
     2461 ttcaacgaac tcaactgcct cagctggttg agtatacttg tgaacatcag aagtaacatc
     2521 atcttctgtt ccagctagaa caccaagttc tgcttcaaca acaacacctt tagagtgtgc
     2581 atattcaaca gcttctttag ttattctaac attttcttca aagtcaaaat gagaaccatc
     2641 tatcataact gaagagaaac cagcatcaat acaagtttta atagcatcca tatttggacc
     2701 atggtctaag tgaagagcta catctacacc tatttcatca gaagcagcct taaccatttc
     2761 aactaatgta tgaggtccag catatttaac agctgacata gaagcttgta tcataacata
     2821 agaattttta gctttagcag cttttaaaac accttgtaat tgttctaagt cacttatgtt
     2881 aaatgcacct atagcaaatc caccttcgta agctttttta aacatttctt ttgtagtaat
     2941 taatgccatt ttaaactcct cctattaact tttaaaattt acccatcact tatattataa
     3001 cgttaaatca aaaaaagtat atagtaaatt gtaataacta taaattacaa gtataatgtt
     3061 aaatagaaga caataggatt aaataaaata ataaagaata taaataattt aggaaaatta
     3121 gctatataat gctaactata agggtaggaa ctaccttatt agtttggaga tactgataac
     3181 aatagttgtc ttgaccaaga agttaccact tcaaacgtta tcgaagggaa ttaagtggat
     3241 gataattcac taaaaattta aaataaagga gataaactat gccacagata aaaataagag
     3301 gaattaatga aaatgatata tgtaaaataa gtaaaaaaat gataaatgat ttagttgaag
     3361 ctgtaaaatg tccaagagac tactttgaaa tagaatgtat aaagtcagtt gcaataagag
     3421 atggtaaaat agcagatgta tatccttttg ttgaagtagc ttggtttgat agaggacaag
     3481 aagtacaaga tatagtagca agaataataa ctgatagtat aagaaataat ttagatgtag
     3541 aaagtatgga tttagcattt acagtatttg aaaaagaaaa atattatgag aatggagagc
     3601 atttttaatg gctaaaaagc aacgtataga taaaattctt tcaaatttag gttatggaag
     3661 tagaagtgaa ataaagaagt attgtaaaca agggtctgta gtagtaaatg gaagtgaagt
     3721 ttcaaatcca ggaactcaag tagacactga aaatgatgag atattattta atggagaaga
     3781 agttatatat agagaatata tatatttgat taaaaaatag ctacgtacac tcgtgacttt
     3841 agtcatgagt tagtagctaa aatagtcagc atatagggaa ac
//
LOCUS       DACTGW010000279         3827 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.282, whole genome shotgun sequence.
ACCESSION   DACTGW010000279 DACTGW010000000
VERSION     DACTGW010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.282"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            473..2071
                     /locus_tag="I9573_003378"
     CDS             473..2071
                     /locus_tag="I9573_003378"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HAT4797964.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFDVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(2306..2497)
                     /locus_tag="I9573_003379"
     CDS             complement(2306..2497)
                     /locus_tag="I9573_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HAT4797965.1"
                     /translation="MAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQ
                     SAGTAATLTIFRIADTVMT"
     gene            complement(2666..3259)
                     /locus_tag="I9573_003380"
     CDS             complement(2666..3259)
                     /locus_tag="I9573_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HAT4797966.1"
                     /translation="MRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYY
                     NLRTITWNDRVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNY
                     PYQIFEFTSSRDCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSIGPMG
                     ATGPTGATGNSSQPIANFLVNAPSPQTLNNGNAITGW"
     gene            complement(3260..3406)
                     /locus_tag="I9573_003381"
     CDS             complement(3260..3406)
                     /locus_tag="I9573_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HAT4797967.1"
                     /translation="MRKIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFF"
BASE COUNT         1406 a          499 c          493 g         1429 t
ORIGIN      
        1 tgtgcaattt ttctttttat aacaactgta tggttttaaa aaatatacaa actaaaatta
       61 ttctaatctc tataagttat ttttaagtca atatgtaata attatgcttt tcatatagat
      121 aaataatttt tatattaaga ccgttaattt tttaatgata atatatatac aaaatttttt
      181 acagtataaa atatttataa atttttaatt tattaacata ttaattaatt ttttcccagg
      241 tagatttaat caatttattt taaataaaag taaatttgat tctatgtttg gttgggtaaa
      301 aatatatata ataataattt ttatattaaa tttgtagtat ttatgtaaga aataaatttt
      361 atataatatt ttttcactaa atttttttct ttttacaaca aatactcact accttttgat
      421 ataatatttt tatcaattat tattatattc aagttttgaa aggagtatta aaatgagttt
      481 attagttttc ggacataaaa atccagatac agattcaata tgttctgcaa tttcattagc
      541 acatttaaag aacaaactag gaataaaagc aacagcttat gctttaggag atatcagaaa
      601 agaagctaaa tatgccttgg attactttaa ggtagatgca cctgaaattt tagataatgt
      661 tagaatacaa gttagagatt taaattatga taaagtagta cctttaacac caacttcatc
      721 tatattagag gcttataacc ttatggatga aaaaaatgtt aaaacactac ctgtaactta
      781 tgatgatgga acttttgctg gtatcattac tatgaaagaa atagctaaaa atctacttca
      841 tcaacacttt acaactatac acacatcact atcaaacatt tgtaaaaacc ttaatggtac
      901 tattcttgtg gactgtggtg aagatataaa aggtagagta gtcactttat catttggaat
      961 ggagactcta atagagactt taaaagaagg agacatagct attgttggag atagatatga
     1021 tagtattgaa tatgctattg atacaaaagt taaactttta atacttacta atcatacaga
     1081 gatttcaaaa gatttgctag ctcttgctaa gataaatgga gtgtctgttg tttctgttga
     1141 aagtgacaat tataaaactt ctaatgtaat aaatcaatgt agtttcttag actctataga
     1201 agctacagaa aatttaatta cattcaaaga agatgattat atggatgaaa taaaagagat
     1261 tatgctagaa acaaacttca gatcttatcc aatattagat gatgataata agtttttagg
     1321 tttagtttca aaaggtcatc tattaaaccc atctaaaaag aatgttgttt tagttgacca
     1381 taacgaatat gctcaaagtg ctgatggaat tgaacaagca aacatagttg aaataattga
     1441 ccatcataaa cttggtggta tttctactga tgttcctatg tcatttagag ttatgcctgt
     1501 tggatgcaat agtactataa tttatcaaat gtacaaagaa aacaatgttg agatacctta
     1561 tgaaatagct gggcttcttt tatctgctat attatcagat acattattat ttaagtctcc
     1621 aacaacaact gatatggata agaaagcttg tgaagaatta agtaaaatag caaaagtaga
     1681 catggaaaaa tacgctatgg atatgtttaa atgcggtact tctttagatg aatatactat
     1741 agaagaaata gtaaatatgg attttaaaga atttaatatg agtggacata gagttggtat
     1801 tggacaagta tttaccttag acatagactc tatatttgct aaaaaagatg agtttttatc
     1861 ttatataaat tcgactgatt atgacaaact aattcttgcg gttacagata taatcaaaga
     1921 aggttcttat ttaatctata aagcagaaga tagcttaatt gctaacgcat ttgatgttga
     1981 aggtgttcaa ggtacatttg caccaggtgt tgtatcaaga aaaaaacaat tagtccctaa
     2041 tttaactact gcaatcaaaa attttaaata atataaaatt tttaaataac gaaaactgtc
     2101 tcaaatctat atttgagaca gttttttatt atacatatat tgataataaa gttaaataaa
     2161 aatccataaa tttaaaattt ttatgaatat attttattat tattgttatt ttattcattc
     2221 taaaataggt aaaactcaat atataaaaat acttttgtca tttaaataaa acctaaatag
     2281 tttttaagat tattttagac acgttttaag tcataacagt atcagctatt ctaaatatcg
     2341 tcaaagtagc cgcagttcct gctgattgcc catttcgtat ccctactgtt gtagatgaaa
     2401 aattatttat tgtaaaagta gtccccccaa ataaaaaacc agcaaaatat ccagaaagta
     2461 ctcctccgcc tcctggctca gtagtaagcc ctgccaaatc ttttcctcct aaaattggga
     2521 ataggatagc aaaagaatat tgatttatac ttgatgaacc tggttgtaat gctactgaaa
     2581 ctgatatata atacacacca ttttcttgta ctgtaaacgt accatttgca tctactgtta
     2641 tacttgaact atttcctatt attgtttacc aacctgttat agcatttcca ttatttagtg
     2701 tttgtggaga tggtgcattt acgaggaagt tagcaattgg ctgagaggaa ttgcctgttg
     2761 ctcctgttgg acctgttgct cccattggtc ctatacttcc tgtcgctcct cttggtccca
     2821 ttggacctgt aattctaatt ggaagacact cacatttagg ataacaatta tctataattc
     2881 tatcttcaaa tgttacaagt atatacggtt tgttacaatc cctactagat gtaaattcaa
     2941 aaatctgata agggtaattt aaagataaag ctaacccata attaggatat ttattcattg
     3001 cccatgcttt tatagttccc gtaatattta aacaaatata gttggatgta tcacttatcc
     3061 caattggtaa atatcccctg atattttcca tagacactct atcattccat gttatagttc
     3121 gtaaattata atattcactt attcttttaa tttcaatatc tactttttct ttaaaaatat
     3181 gattcttatt tgaatcaata tgaataaata actctgcttt taatatactt ttgtctttta
     3241 atatatctgg tattctcact taaaaaaaag taatcaactt ttcttttaca aaactatttg
     3301 aacatttact tcctattaag caataatcta aattactaaa gtttttatct ggatattttt
     3361 tagatataaa agtatcatca tttaaataaa gtataatttt tctcatttat aatccctcca
     3421 ttttgggtct atatcatact atgtttaaaa tacatgctta gttacaatac aattaaaatt
     3481 tatagtatta aatttttata taataatgct gatttctaca aaaaaagaac atgtcattag
     3541 aaacttctaa tagcatgttc tttttcatta ttatatatta cactttaaat tattccttga
     3601 ctggcttagc tggtgtaaac taaaccagtc tttctaaaat attaagcact tttttcttat
     3661 tatgtttttt aggatttatt gtctctatta ttgtaaacca aagttataaa attgtaaata
     3721 gaatatatta ccataacaat atttttttat aagtaaaaat tataaaaaac cttattttta
     3781 ttaaatagaa taaataaaat aagtttaaaa aatactgaaa taagtgt
//
LOCUS       DACTGW010000280         3811 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.283, whole genome shotgun sequence.
ACCESSION   DACTGW010000280 DACTGW010000000
VERSION     DACTGW010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.283"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            139..1056
                     /gene="pfkB"
                     /locus_tag="I9573_003382"
     CDS             139..1056
                     /gene="pfkB"
                     /locus_tag="I9573_003382"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAT4797968.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYAKRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            1093..3012
                     /locus_tag="I9573_003383"
     CDS             1093..3012
                     /locus_tag="I9573_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005806477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAT4797969.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            3584..>3811
                     /locus_tag="I9573_003384"
     CDS             3584..>3811
                     /locus_tag="I9573_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAT4797970.1"
                     /translation="MLNLQSKWVNFLKPTYVSDEIKRKKLQVIIFFSITYGLSYSLGL
                     LAICFNHLIDSEILAQFMMILPLSSVSIAKFY"
BASE COUNT         1477 a          395 c          708 g         1231 t
ORIGIN      
        1 atttggataa tataaaacta aataagataa aaacgataaa tttctctttt aattaagaga
       61 aattttttga ctttcaagga tattaatata aagttaagat ataaacataa agcaaataaa
      121 taaaaaagag gtggcaatat gatttataca gtgacattaa atccatcaat agattatata
      181 gttaagctag atgagctaaa gacaggaagt acaaatcgtg taaaagaaga atacgtttat
      241 ccaggaggaa aaggtataaa tgtatctcgc atacttaagg aacttggaaa tgataataca
      301 tctttaggtt ttataagtgg ttttactgga gaatatataa taagaacatt ggaagaaaaa
      361 gaattgaaga cagattttat aaagataaaa agtggttttt caagaataaa tgtaaaaata
      421 aaggcgttaa aggaaactga gattaatgga caaggtccaa atatagatga tgaagacatt
      481 gatactttat ataaaaaact tgataagcta aatcaagatg atattttaat attggcagga
      541 agtataccat ctactttaga tgaaaaacta tacgaaaata taatggctag attagaaaaa
      601 aagaatataa aagttgtagt agatgcaact aaaaatcttc ttcttaatgt tttaaaatac
      661 aaaccttttc taattaagcc taataacgac gaattagaag aactatttgg tgttaaatta
      721 aattcaattg aagacatggt aaaatatgct aaaagattaa aggaaatggg agctataaat
      781 gtcttggtat caatgggaaa agatggagca ttgttgatta cagaggaaga tgaagtgctt
      841 ataagtgatg tccctaaagg aaaagtaaaa aattctgtag gagcaggtga ttcaatggta
      901 gcaggattta tatctgggta tttgaatact gggaaatatg attatgctct taaattggga
      961 gcagcttcag gtagtgcaac tgctttttca tatgacttag ccaaaagaga atacattgat
     1021 aaattggtaa atgagatatc tgtaaagcag ttttaaaata ttaaaaaata tatagaaagg
     1081 taattaatat ttatgaaaat tatagattta ttagatgaaa agtcaattaa gttaaattta
     1141 aattcaaaaa ccaaatcaga agcaatagaa gaattagtag atttggtcgc aaatagtgga
     1201 aatttaaatg ataaagaaaa ctacaaaaaa gcaattttgg ctcgtgaaga aatgagtaca
     1261 acaggaattg gagaaggtgt agctatacct catgctaaaa attcttcagt tactaaagct
     1321 tgtatagcag cagcagtgtc aaaagaaggt atagattatg aatcgtttga cggaagttta
     1381 tcaaatctat tttttatgat agctgcacca gacggagcta acaatactca tcttgaagta
     1441 ttatcaagat tatctacaat acttatggat gaagacttta gaaataaact tataaattca
     1501 agctctgaaa aagaatttaa ggaaataata gataaaaagg aaagagaaaa attcagtgaa
     1561 gaatatcaag atgagaaagt ggctgaaaaa aatataatag atactaaaga gaatgatgct
     1621 aataagtatc ctaaggtttt agcagtaaca gcatgtccaa ctggaatagc acatacattt
     1681 atggcagctg aaagtttaaa taaaatggca gaaaacaaag gtgtatcaat aaaagtagaa
     1741 actaatggtt cagcaggtgt taaaaataag cttacaaaag atgaaataga aaatgcaaca
     1801 tgtataatag tggcagcaga taaaaatgtt gagatggcta gatttaatgg caaaaaagtc
     1861 ataaaaacta aggtggctga tggtatacac aaagcggaag aacttataga caaggctgta
     1921 aatggagatg ctccaattta ccatggagga gatggaagtc acaacgaatc aaatgaagaa
     1981 agtgaaagtg gatttagaaa agtatacaaa cacttaatga atggtgtatc taatatgtta
     2041 ccatttgtta ttggtggagg gatacttata gcgatagcct ttttacttga tgattatact
     2101 attaaccctt caaactttgg ttcaaataca ccgatagcag cgttttttaa agggatagga
     2161 gataaagctt ttggatttat gttacctgta cttgctggat atatagcata ctctatatct
     2221 gatagaccag cgtttgttgt aggatttgta ggaggtgctc tagcaggtga tggtggttca
     2281 ggatttttag gtgcactact tgcaggtttt atagcaggtt atttagtaga agggcttaag
     2341 aaaatattta gtgtactacc agcttcattg gaaggaataa aaccagtatt gttataccct
     2401 ttacttggaa cacttttgat gggaattata atgacatttt tagtaatacc tccagttaca
     2461 gcaattaata atgcaatggt tggattttta aatggtttag gcggaacttc aaaaatagtt
     2521 ttaggattag tattaggtgg aatgatggct gtggatatgg gtggacctgt aaataaagca
     2581 gcatatgtat ttggagttgc atcacttgaa tcagggcagt ttgaaataat ggcagcagta
     2641 atggctggtg gtatggttcc gccacttgct atagctcttg caactacatt ttttaagaat
     2701 agatttacta aagaggaaag agattctggt aaagttaatt atgtaatggg attatcattt
     2761 gttactgaag gagctatacc ttttgcagct ggagacccac ttcatgttat accagcttgt
     2821 gttggaggtt cagcagtggc aggagcactt agtatgttgt tcaacgcagc tcttcgtgca
     2881 cctcatggag gagtatttgt tataccagtg gttacccatc catttgcata tatattagct
     2941 atagctgtag gtgctttggt tggaatgatg cttttagcat tacttaaaaa accattaaat
     3001 caagaagtat agcaattaaa ggatattcaa gaacttaaaa tatgatgttt tataaaaatt
     3061 aatatattgc tgaattattt taaaataagg attttattaa aataaggatt ttattaaaat
     3121 aaggatttta tgaggctgtt atataacgat ttgtaattgt tatcttgaca gtcttttatt
     3181 tttatataag taaaataagt gtgttttgct ttatttgtat ttaaattgtt tttaagttag
     3241 aatataaata aaattatata ttattttaat attcactcga actagaaaat aatatttgtt
     3301 gtattttttt gtatataaaa atatattgta atattaaaaa aataatagta aactgattgc
     3361 ataaaaatga tttttatata aattttaaac agaagaataa atttctaacg aaattggata
     3421 tttcttctgt aaatacatat tcaaaaaaag aattataaga gtttagtcag cagtcgtgta
     3481 agtaataagg actttataaa tatatagaaa tataatgata tgagtataag tagaaaaatt
     3541 ttatgatttt tacaattatt ttttataaaa agggaggtta tgtttgttga atttacaatc
     3601 aaaatgggta aactttttaa agccaacata tgtcagtgat gaaataaaaa gaaaaaaact
     3661 acaagttata atattttttt ctataactta tggtttatct tattcactag gtttattagc
     3721 aatatgtttt aatcatttaa tagattcaga aattttagct caatttatga tgatattgcc
     3781 attatcttca gtttctattg caaaatttta c
//
LOCUS       DACTGW010000281         3793 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.284, whole genome shotgun sequence.
ACCESSION   DACTGW010000281 DACTGW010000000
VERSION     DACTGW010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.284"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            23..1135
                     /locus_tag="I9573_003385"
     CDS             23..1135
                     /locus_tag="I9573_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAT4797971.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1343..1819)
                     /locus_tag="I9573_003386"
     CDS             complement(1343..1819)
                     /locus_tag="I9573_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797972.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQPNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2018..3643)
                     /locus_tag="I9573_003387"
     CDS             complement(2018..3643)
                     /locus_tag="I9573_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAT4797973.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1322 a          609 c          421 g         1441 t
ORIGIN      
        1 aattttgatt ggtgggttat atatgaatgc atttattaga aaaagaaata aaaactatgt
       61 tgtttacctg gaatttgaag atgaaaagac aggtaaaaga aaacaaaaaa atatgggtac
      121 ttttgataaa aaaagggaag caagtaaaag actaaatgag gtaaaagaaa gcttatataa
      181 tgatggtttt ttagtaccaa gcgaaattac agtgtcagaa tttctattag attttctaaa
      241 taaatatagt gaaaatatat ctttagctac atataataat tatgttagta tttgtaaaaa
      301 ttacattaat ccatctattg gaaaatataa aatacaagac ttacatccgg ttcacataca
      361 aaactacata gataaactat cttataaact aaatcctcaa tcaattaaga ttcatataaa
      421 tatattgaaa cttgcaataa aaagagctta tagactcaaa ttaataaaag aaaatgttat
      481 ggatagtata gaggctccaa gatataaaaa atttaaaaat gagatttatg atagagaaca
      541 aatgataaaa ttattagatt tagcaaaaaa tacagaaatg gaattaccta ttaatttggc
      601 tattggatta ggtcttagaa tttcagaagt tttaggtcta acttgggata atattgattt
      661 tgaagaaaat acaataacag taaataagat aacttcccga attaatggtt ctgttattct
      721 caaagagcca aagacagaaa gctctgttag aaaaatatct tctcccaaag aattaatgtc
      781 tttattaaaa gaatataaaa taaaacagaa taaaaattta ttaaaatctt ccattagaaa
      841 taataataat ttgttgtttt tcaataagaa atgtgaacct attgctgaag atgttatgag
      901 taaaaaattt aaaagatttt tagaaaagaa tgaattacct catattagat tccatgattt
      961 aagacactca catgttactt tacttataaa ttctaaagta cctataaagg ttatatccga
     1021 gagagtagga cactcaaata taagtactac tctaagcgtt tattctcatg tacttaaaga
     1081 aatggataaa gaggcttctg acaaaatttc tgaaagttta tttaatgcta attaatatta
     1141 atttatctta gctttaaatt agcttagctt attgagatag attcattaat attaaatgta
     1201 tctatctcaa attaataact acttaaataa tctgttttat tttaatttat cgcctcagtt
     1261 attatacttt tttatgaaaa attatacttt acaataaaaa agatatttcc ttatagaaag
     1321 tatcttttta aatactattt tattatatac ttgctaacat tggaatatct aacatcttct
     1381 tcattacatc atctactttt tttgctttat attcattgta tccaattaaa tatccttttt
     1441 catatgaact ttttaaaatc tctttttcat ataaattatc agatttggct tctgtgacac
     1501 ttaaattata aagcccttta cacccatctt cataaccttt attataccct ttttctgcaa
     1561 aatagttttt atgatttttt tcgttatgta ctgaagatat tgttactcca gaattttctt
     1621 ttgtactagc ataaggacaa aaacctttat ttggttgatg tgctgaatgt cctccacaat
     1681 aataacgtgt taactctgat ttatttttat tatgatgacc tcctgatgaa ttatgccctc
     1741 ctgagtgagc atatgaaaaa ttccctacac taatcatagc taaactcaat actacagcta
     1801 ctaacctttt cttaaccata atctattccc cccaaaatgt tatatatttt tattatatca
     1861 tctactatat tatatttaaa ttcttaatat acaaaatatt actcgattct gctaataaac
     1921 atatttaaac aacataaaaa acctcctaat atttgattaa aaatttacac actcatgcat
     1981 agcatgtatc aaatattaaa aggttttata ctaataatta tttatctttt ccatacattt
     2041 tagctaattg gtcttgtatc tctttacttt ctaaatactc atcaaagtcc atttttctat
     2101 ccattattcc atttggagtt atctctataa ttctatttgc tatagtagat ataaattcat
     2161 ggtcatgaga agtaaataaa agcactcctg ggaacttttc aagtccttta tttacagaag
     2221 ttatactttc taggtctaag tggttagtag ggtcatctaa tacaagtaca tttgcatttg
     2281 aaagcattag cttactaagc atacatctaa ccttttctcc tccagatata acttgagctt
     2341 cttttaatgc ttcttcacct gagaataaca ttctacctaa aaatcctctt ataaaacttt
     2401 cacttttttc ttcagagaac tgtctaagcc agtcaaccaa agaatattct actccgtcaa
     2461 aaaacttatt atgatttttt ggaagataat cttgagaagt agttactccc catttatact
     2521 ctccactgtc tggttcaagt tctcccataa ctatgttgaa aagtgcagta gtagctattt
     2581 catctcccat aaatactact ttatcatctc tatccaatct aaatgacaca ttatcaagaa
     2641 ccttaacccc atctatagtt ttagttaaat tgtgtacttc taaaacttca ttcccaattt
     2701 ctcttgcagg tgtaaatcct acatatggat atcttcttct tgatggttgt atatcttcaa
     2761 cttctaattt ctctaactgt ttctttcttg atgttgcttg tttagccttt gaagcattag
     2821 aactaaatct agctataaat tcctttaatt gagctatctt ttcttcagtc tttttatttt
     2881 ggtccttagc cagttgtaaa gccaattgac ttgactcata ccagaaatca tagtttccaa
     2941 cgtacatttg tattttacca aaatctacgt caactatatt agtacatatt tgatttagga
     3001 aatgtctatc atgtgatact attataacta tagactcttc taaatccatt ataaaattat
     3061 ttaaccagtt tattgattta aaatctaagt ggtttgtagg ttcgtccatt aatagtattt
     3121 caggcttacc aaaaagtgat tgtgctaaca ataccttaac cttttcccca ccaactagtt
     3181 ccttcatttg tttgtaatgc atatctttcg ttatgccaag acccataagt attttctcag
     3241 cattagtttc tgcatcccat ccgtcaagct ctgcaaactc accttcaagt tcggcagctt
     3301 ttatcccatc ttcttcatta aagtcttcct tcatatacaa tgcatctttt tctttcatta
     3361 tgttccaaag tctctcatga cctcttataa caacatttaa aacagtttct tcttcatatt
     3421 caaagtggtc ctgttttaaa actgacattc tttctttatc agttatagat atactacctg
     3481 tattaggctc tatttctcct gataatattt ttaaaaaagt agattttccc gcaccattag
     3541 ctcctattat tccatagcaa ttacctttag tgaattttaa gttaacatct ttaaatagtt
     3601 ccttatcacc aaatctaagt ccaacacctg taacttgtaa cattaatata ttttcctttc
     3661 tttttgctca taatttattt tatcataaaa ctttgataac atttttatta tatattatca
     3721 cgtaaatagt atatttttta tactaaattt gtgaaatatt tattaaaata atactaaaaa
     3781 ttgtaaattt ata
//
LOCUS       DACTGW010000282         3757 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.285, whole genome shotgun sequence.
ACCESSION   DACTGW010000282 DACTGW010000000
VERSION     DACTGW010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.285"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            235..384
                     /gene="rpmG"
                     /locus_tag="I9573_003388"
     CDS             235..384
                     /gene="rpmG"
                     /locus_tag="I9573_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAT4797974.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            410..631
                     /gene="secE"
                     /locus_tag="I9573_003389"
     CDS             410..631
                     /gene="secE"
                     /locus_tag="I9573_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAT4797975.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            651..1193
                     /gene="nusG"
                     /locus_tag="I9573_003390"
     CDS             651..1193
                     /gene="nusG"
                     /locus_tag="I9573_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HAT4797976.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDVDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1229..1654
                     /gene="rplK"
                     /locus_tag="I9573_003391"
     CDS             1229..1654
                     /gene="rplK"
                     /locus_tag="I9573_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAT4797977.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1724..2422
                     /gene="rplA"
                     /locus_tag="I9573_003392"
     CDS             1724..2422
                     /gene="rplA"
                     /locus_tag="I9573_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002846502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAT4797978.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     gene            2645..3151
                     /locus_tag="I9573_003393"
     CDS             2645..3151
                     /locus_tag="I9573_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAT4797979.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3209..3574
                     /gene="rplL"
                     /locus_tag="I9573_003394"
     CDS             3209..3574
                     /gene="rplL"
                     /locus_tag="I9573_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAT4797980.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
BASE COUNT         1521 a          446 c          749 g         1041 t
ORIGIN      
        1 atggattaaa aggaataaca aaaagagggg ttaggctcct ctttttttaa catagaaata
       61 caaaaacatt gacacagcat aggattttgt gataacttat acaaggtaat gattatagga
      121 agttaataat atcttgaata ctgtagattt ttaataaata catattttaa agatattata
      181 ttcacatata ttaacaatat atattattgt attacaaagt tggaggtgta tcagatgaga
      241 gttaaagtaa ctttagcatg tacagagtgt aagcaaagaa actacaacac tactaagaat
      301 aagaaaaata atccagacag aatagaatta caaaaatatt gtagattctg taaaaagcat
      361 acaactcata aagaaacaaa atagtttttt ataaggatgt gagtatggaa tggctgccca
      421 aaaaaatgac ggtgcaaaaa ctaaaaaaag gtttagttta tttggatatt taaaagaaac
      481 taagcaagaa ttaaaaagag ttacatggcc gacaaaaaag gaactgttta aaaacacaag
      541 tatagtttta actgtagtta tttcatgcac tatattagta tggggtatag acactatatt
      601 atctggtgca ctagcgttat tactaaaata gacgaaaaga ggtaagttaa atgtcagaat
      661 tacaagaagc aagttggtat gtagttcata cctattcggg acatgaaaat aaagttaaag
      721 ctacaattga gaaagcagtt aaaacaagag gtatggaaga ctgcataaga caagtagttg
      781 ttccaacaga agaggtagtg gaaactacta aaactggaaa agaaaaaact agacaacgta
      841 aagtttatcc aagttatgtc ttggttaaaa tgataataac tgatgaatct tggtatgtag
      901 taagaaatac taagggagtt acaggtttcg ttggacctgg ttcaaaacca gtaccattaa
      961 gtgaagatga agtcaaagct atggggatag atacaacaga ccctaaagta gtaaatagcg
     1021 atgttgattt tgaaatagga gacacagtaa aagtatctca aggaccattt agtggacaaa
     1081 taggtaacat agaagaaatt gacttagaaa atagagaagt caaagtatgc ataaatgcat
     1141 ttggtaaaag gactctattt gtaatagagc ttgaaggtat agaaaaaatc taatactata
     1201 tagaaaaatc tgaggaggtg cgctcaaaat ggctaaaaaa gttataggtc aaataaaatt
     1261 acaaatacct gcaggaaagg ctactccagc gccaccagtt ggaccagcat taggacaaca
     1321 tggtgttaat ataatgggat tcacaaaaga atttaatgct aaaactgcag atcaagctgg
     1381 aatgataata ccagttgtta taactgtata tcaagataga tcattcagtt ttataacaaa
     1441 aactccacca gctgcagttt taattaaaaa agcattaaac ttaaaatcag gttctggaga
     1501 accaaacaaa aagaaagttg ctaaaatgac ttcagctcaa gtaagagaaa tagctgagtt
     1561 aaaaatgcct gacttaaatg ctgcatcagt agaagctgct atgagtatga tagctggtac
     1621 tgctagaagc atgggtgttg taatcgaaga ctaattaaaa gatacacttt ttttataaag
     1681 tgggaggcga gaagccgaaa ttaaccacaa ggaggaaaat aaaatggcta aaaaaggtaa
     1741 aagatatgca ggtgcattac aaaaagtaga tagaactaaa ttttatgatg catcagaagc
     1801 attaacatta gtttcagata tagctggagc taaatttgat gagacagtag aagcacacat
     1861 taaattaggt gttgactcaa gacatgctga ccaacaagta agaggtgcag ttgtattacc
     1921 tcatggaact ggtaaaacaa aaagagtttt agtttttgct aaaggtgaaa aagctaaaga
     1981 agctgaacaa gctggtgctg actttgtagg agctgaagaa ttagttcaaa aaatacaagg
     2041 tgaaaactgg tttgactttg atatagtagt tgctactcca gatatgatgg gtgtagtagg
     2101 tagattaggt agagtattag gacctaaagg tttaatgcca aaccctaaat caggaacagt
     2161 tacatttgat gtagctaaag ctatagatga aataaaagct ggtaaagttg aatacagatt
     2221 agacaaaact aacataatac acgttccagt aggaaaagta tcatttggtg gagaaaaatt
     2281 aactgaaaac tttactgcat taatggatgc tataataaaa gctaaaccag ctgctgctaa
     2341 aggacaatat ttaagaagta taacagtagc ttcttcaatg ggacctggag ttaaaataaa
     2401 cccagcaaga acagctgaat aataaaactg ttgacacagg ttgacaaaaa tgttattata
     2461 tcacttgtca atgtaaaaaa gaatagattt gccgtagata gtgggtgcga aagcttaata
     2521 tcccgccgag gttttagata aattgagatt actaaagctt ttgatgtcta cttttggcaa
     2581 caaaagcttt ttataatgtt gcggtaaata ttaagcctat ttggtgaagg aggtgcacat
     2641 ctatatgaga aaagctatag aaattaaatc agaagttgtt tctgagatag ttgaaaagtt
     2701 acaaaaatct tctgctgctg ttgttgtaga ttacaaagga ttaacagttg aagaagtaac
     2761 tgagttaaga aaacaaatga gagaagctgg tgtagattac aaagtataca aaaatacttt
     2821 agttagaaga gcagctaaag aagttggtat agagcaattt aatgatgaat tattagttgg
     2881 aactaatgcc atagcatttg gatatgatga tccagttgct ccagctagaa tattaaaagg
     2941 attcatggat tctcatccaa aaatgaaatt aaagatgggt attgtggaag gtgcattcta
     3001 tgatgaatct aagattgttg aaatggctaa cataccatca agagaagttc ttatcgctaa
     3061 attacttggt agcttaaaag ctccagtatc aaacttcgca tacttaatag atgcaatagc
     3121 gaaaaaggca gaaggtcaag aagaagctta attgcttaac taaaaaatta taaatattaa
     3181 tttgaaataa aattcggagg tgctaaaaat gacaatagaa caaatattag aagctataga
     3241 aaatatgaaa gttttagaat taaatgaatt agttaaagct gctgaagaaa aattcggtgt
     3301 atcagcttca gctccagtaa tggtagctgg tgcagctgct ggtggaccag ctgctgaaga
     3361 aaaaactgag tttgatgtag tattaactga cgttggttct tcaaaagttg gagttataaa
     3421 agcagttaga gaaataacag gattaggatt aaaagaagct aaagaagtag ttgacaatgc
     3481 tcctaagaca gttaaagaag gagcttctaa agaagaagct gatcaaataa aagagaaatt
     3541 agaagctgct ggagctaaag tagaagttaa gtagtttctt taaaaagata cccattgggt
     3601 atcttttttt atttataaaa aactataaag aaagtatttg gaattataaa gaaagtgttt
     3661 ttataaaata aaaaaaattt atataatagt actaaattaa gggtatcata tatggtgtaa
     3721 tggtatttta atttaacatt attatcaaat taaaata
//
LOCUS       DACTGW010000283         3717 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.286, whole genome shotgun sequence.
ACCESSION   DACTGW010000283 DACTGW010000000
VERSION     DACTGW010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.286"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            51..719
                     /locus_tag="I9573_003395"
     CDS             51..719
                     /locus_tag="I9573_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797981.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWENDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            878..1384
                     /locus_tag="I9573_003396"
     CDS             878..1384
                     /locus_tag="I9573_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HAT4797982.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1512..2528
                     /locus_tag="I9573_003397"
     CDS             1512..2528
                     /locus_tag="I9573_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HAT4797983.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2882..3586
                     /locus_tag="I9573_003398"
     CDS             2882..3586
                     /locus_tag="I9573_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HAT4797984.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVIICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1594 a          318 c          647 g         1158 t
ORIGIN      
        1 tatatattat attaggtatt ttaaataaat tttttaaagg gtgatttttt atggaaaata
       61 taactagtta ttttacaaca atactatgtg tatttatatg cttatcatct gtatttatat
      121 ttactcaact agctagagta tttattaata agaagaagat taatcaaaaa attaaatcaa
      181 gaaacggatt taggtatgat agagatttta ttgaagccag aagagaagaa atacatataa
      241 aggataacaa taataataaa aataagagta acaataagaa attaaaggaa gaaaaagtat
      301 ttaaatacga taatggtgat ttatataaag gtgagtttgt tgatggaaag aaaaatggat
      361 ttggaatata tattttttca agtaaggaaa agtatgaagg attatggaaa gatgacaaga
      421 tgcatggaat aggaaaatat acttatagag atggttcaat atatacaggt gaatttaaat
      481 acggattaaa aaatggtttg ggaaaattaa catatcccaa taatgacatt tataagggat
      541 attttttaga taataagaaa aatggtaagg gtgtattata taaaaatgat ggaaataaac
      601 aagcaggaat atgggaaaat gatgaacaat gtaaaagttt agattttaaa gatttaaata
      661 ataataaaat aaataatgtt tatcaaaata gattaaataa caataaatta aaagtttaat
      721 tattctaata aaaaaatctt tatttatgat ggaatatagg atatatctat gttgtaattt
      781 gtaaaatctt cctatatcat ataaaattaa gtattgatta gtctattaca taaatataga
      841 atataaatat atccatttaa taataaggag aagagatatg aaagaatatg tagttgtgga
      901 tttagaaact acaggattag atccatataa aggttgtgaa ataatagaaa taggaataac
      961 agaaataaaa aatgaacaaa ttgtaaaaaa ctattcaagg ttgataaaac ctaaggggat
     1021 aatttcttca tttataacag aattgacaca tataagtaat gaaatggtag aaaatgaaga
     1081 accactagaa ttagttttac ctagatttag aaaatacata ggtgatagaa ctataattgc
     1141 acacaatgcg aaatttgatt taaaattttt aaattactat cttaggatgt tagatttaga
     1201 accaataaac aattatatat gtacagtaga attattaaaa aaatgtaaaa gttataaagg
     1261 aaaaaacaag aaacttgaaa cagcttgtgc ttattacaac atagagaata taaatgctca
     1321 tagagctgac agtgatacat tagcaactgc aaaactattt ttgaaaataa aagatgaata
     1381 ttaattataa atatagagaa aattagtgaa aatatccttt ataaaattga cccttaagct
     1441 tacaattttg ttctttgatt tattttgtag aacaatgtat aatgtacctt atatgaagaa
     1501 gggagactaa aatgggttat tacaatgata atgataataa taaaataaga cgtaaaaaaa
     1561 ttagtaacaa caaagatagt aataaaaaat ctaaacattc ttatacttcg gcaaataaaa
     1621 aaaataaaag tcctaataca agttccagta aaagaatgag tgaaaaagta gattcacaga
     1681 atcttataaa aagagataga gatagtttta caagagataa tgtaagacct ttaaatacag
     1741 attacaatca aagagaaaga agaagagtcg agtataaaca aagagagaaa aaattaaaaa
     1801 gtaaaaaaag aaaaaaacaa aaaaggatta gaatagttag ggcattatta tctattatgt
     1861 tagttgcact aattatattt tcagtggcag gtgcaatatt tacagtttca gctataaaag
     1921 ggtcaccaga agttactaaa aagttaatta ggtctaaata tataagtagt gaggttgtaa
     1981 gtataaaaca aatgccaaat gatttaaaac atgcaatagt ttccatagaa gatgagagat
     2041 tttataagca taaaggagta gatgttattt ctctagttag gtcagtatta aacaatgtgt
     2101 ttacagatac aacacaaggt gcaagtacta tagatatgca agtttcaaaa aacctgctta
     2161 ctagtaatga taaaactatg aagcggaaaa taagggatat gtataatgct aagcagatga
     2221 ataagattat gactaaggat gaaattcttg aggcatattt aaataatatg tatctaggta
     2281 aaaatgcata tggagctgca aagggagctg aagtctactt tggtaaaaaa gttagtgaat
     2341 taaatttagc tgagtgtgct atgttagctg gtatgacaaa taatcctgca aggtatattg
     2401 accatggaga ggctaagaag agacaagtga ttgtactgta taagatgcat cagcttggtt
     2461 atataactga taaggagtat agaatagcaa aagctgatga cactccattt aaatcagaaa
     2521 ttgattaaaa ttgtaagaat atagtatttt caatagttta aacaaacaaa aatgtaataa
     2581 aaaaggtgat ttgccaccca tttgccacca taattataat ctaatggcaa attgttttaa
     2641 tgtacaaact agataataga atattgtttt aaatgctata ttatgctaaa atttaattgt
     2701 attttgtaaa ttaaaaaact tataatttag ttttctaggg ttccgcattt tataatggtc
     2761 aggtccaaga gaaaacgcgc aattgttgtg tacacggaag gataaaagcc tgggagatat
     2821 ttaataatat ttctataggc ttttttattt ttacatattt taaatttgag gaggttttct
     2881 aatgaaaaaa tttgttattg aggatgcatt ttggaatcta tttcctagtg caaaaattgg
     2941 agttattatt tgtaatgata tagataactc tataaaagat gaagactata agaaaataat
     3001 tttgaaggga gaagaagaag cattaaaata cttggaggac tcagaattta gcaacaacca
     3061 agtgattaaa gtatggagag aggcatttaa aaaatttaaa acgaaaaaag gagcaaggtc
     3121 ttctattgaa gcattattaa aaagagttca taatggaaat aatataggaa ctattaaccc
     3181 tttagtggat atctacaatt ttatctcatt aaaatatgca ttaccttgtg gaggggagga
     3241 tatagataaa tttataggag atattaggct aactagagca attggtaatg aggagtttat
     3301 cccactaggg acagatgaaa attcatttcc atatgaagga gaaataattt ataaagatga
     3361 tggtggagca atttgtagat gttggaattg gcgtgaagca gtgagaacaa tgcttactga
     3421 agatactaac aatgcctttt tatgcattga attagtagat gaaagtagat ttaaagaatt
     3481 tgaaaatgct ttaaatgaat tagctaaaat agttcaagaa aaattgggtg ggaattgtaa
     3541 aatttcaatt ttagatattg ataataaaga ggtatcagtg gattaatctg gagatgctgt
     3601 tgtaatggac tattctagtt ataaagatac ttatatttag ggtaataagt tctattttag
     3661 atgtaacgat tatcaaagga tataatatag aaaataaaaa tgtagaatat aaaaagc
//
LOCUS       DACTGW010000284         3681 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.287, whole genome shotgun sequence.
ACCESSION   DACTGW010000284 DACTGW010000000
VERSION     DACTGW010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.287"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            225..599
                     /locus_tag="I9573_003399"
     CDS             225..599
                     /locus_tag="I9573_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT4797985.1"
                     /translation="MIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTTL
                     TLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALHD
                     DENIGKDKLDFLESYFDNLKDE"
     gene            592..1626
                     /locus_tag="I9573_003400"
     CDS             592..1626
                     /locus_tag="I9573_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HAT4797986.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENAVGNNITQIDISTDNNLMLYVAYLWLVVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDVNCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLTDCSLKDKKEYALALIKSIKLSKNYNSGILTTEFNKTSLE
                     KRLDSIVKKKGKNGILVLLILITLFSVTYFNFESISFDNSNSLRNMGILQKSGVDLKE
                     EDDISMRVIN"
     gene            1692..2279
                     /locus_tag="I9573_003401"
     CDS             1692..2279
                     /locus_tag="I9573_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAT4797987.1"
                     /translation="MKNLKKILIITGCLTLISTSLVFANTTTDVDTSKDDDSIISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKNDIIFVNKDSIDANDVENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLNVSNTVVGYNRVESGNPVHLVQLLCRTLDGSSIDDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(2450..3511)
                     /locus_tag="I9573_003402"
                     /pseudo
     CDS             complement(2450..3511)
                     /locus_tag="I9573_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         1374 a          393 c          524 g         1390 t
ORIGIN      
        1 aaatagtttt aatgtgtaaa taacattcta cactattgta attaaatatg taaaaagtaa
       61 aatatttaaa tagatatatt ttaaaatcta acttttaata ttgtaaaaaa attagaaatt
      121 taacaaaaag tacaaaaatt cacatttttt tcataaagtg tttgactaaa aaattcacat
      181 gttgtagaat taaggtagca ggagttttga gggaggtgta aataatgata agaaaaatac
      241 cacaagcaga actaaaagta atgaaattca tttggaaagt agatgttaca gtgacatcaa
      301 aagatgttat tgaagctatg gaacaaaaat atggctggaa acaaactact acactgacac
      361 ttttatctag gctggtaaaa agaggattct tagatgctca aaaaatagac agatatacac
      421 attacacaat aagagtaaaa cacaaagatt atttaagttt tgagacaaag gatttcctaa
      481 gtaatataca tgatgactct ttgagaagct taatttcagc attacatgat gatgaaaata
      541 taggtaaaga taagctagat tttctagaaa gttattttga taacttaaag gatgaataat
      601 tatatgctat atgtttttga aaatcttata aagacaacta ttgtatgtag tcttggtatt
      661 tgtctattat tatttttgaa aagatattta tttaagaagt ttagtaagag attcaattat
      721 tatatttggt tgataatagt ctttagaatg ttactttttc tatttaatta tacaatagtg
      781 tatgaagtaa aagaacccaa agaaaatgca gtaggaaata atattacaca aattgatatt
      841 tctacagata ataatcttat gttgtatgta gcttatttat ggttagttgt aactatagtc
      901 attgcagttt atacatttat aaagtataca agatttaaaa atctagttgt tgatgtatct
      961 tatgatattg aagataatga tgtaaactgt atctacaaaa acttgttgaa agaattaaat
     1021 ataaaaaaga aaattgaatt aagaggttct gatgaattga taagcccagc aggcatggga
     1081 ttatttaaat cttacatatt cttaccagac tatccttata gtaaagatga actaacttgg
     1141 attttgaaac atgaattaat gcattttaaa aataaagata ttttaataaa gtttttagtg
     1201 ttatctgtta gaataatata ttggtttaat ccacttgttt atgtaatgag caataaagta
     1261 aatctagatt gtgaattatg ttgtgatgaa agtgttttga ctgattgttc tttaaaggat
     1321 aagaaggagt atgcattagc acttataaag tcaattaaac ttagtaagaa ttataatagt
     1381 ggtattttaa caacagagtt taataagaca agtctggaaa agagacttga cagtattgtt
     1441 aagaaaaaag gtaaaaatgg aatattggtg ttattgattt tgattacatt attttcagtt
     1501 acatatttta attttgaaag tattagtttt gataattcaa attctttaag gaatatgggg
     1561 attttacaaa aatcaggagt agatcttaag gaagaagatg atattagcat gagagttata
     1621 aattaataga ttaatgtatg taatgaaaag gtatattgtc gtattattaa taaattagga
     1681 ggaattatga aatgaaaaat ttaaaaaaaa ttttaataat tacaggttgt ctaactctaa
     1741 tatctactag tttggtattt gcaaatacta ctactgatgt agatacatca aaggatgatg
     1801 attcaataat aagttcatgt actataactg gttatgaatt tacatatgaa aacgcaccac
     1861 aatctgtaaa atttgattat gaaaaaaaat gtgaagcgct aaatataaaa ccatctaaaa
     1921 atgatataat ttttgttaac aaagattcta tagatgcaaa tgatgtagaa aactatgttg
     1981 agccaaaagc atatattatt agatataaaa aaggttatgt ttttgaagtt actggaccta
     2041 agagttatac attaaatgta agtaatactg tagtcggata taatcgtgtt gaatcaggta
     2101 atcctgtgca tttagttcaa cttttatgta gaaccttaga tggttcaagt attgatgatg
     2161 atagtgaatt tggacctgct acatataatg cagttaagtc acttcaagga agacttggat
     2221 taacaagaga tggaattgtt ggaagaagta cttgggatgc agctggaaga cgtctttaaa
     2281 aagaaagcta ttgcacagat aattttaaat tgctgaatct gtaaacattt acatgtattc
     2341 tttaaataaa aatttatttt ttaagtgata attaaatttt tactaatagg gctatggctt
     2401 taaaaatcat agccttattt tgatatcttt tccatttttg caaaagctat taacccttta
     2461 agaaaatttc ttgttttttt gaaaaatttc tttcttcaaa ataaatactt aaatgagcta
     2521 aaatttgagc acaatttcta attttctgag tccattttta agaaatatat atggttgtta
     2581 agtatagtga tttgtgcaaa taattatcca aagtaaaaat agatttcaat ttagtaactt
     2641 ttcttaataa tctattctaa cttcccattg aatttgtagt atatatcgat gtatttattt
     2701 cttgagagta cttaaaatat gttactaatt cattccaatt gtttcaccaa cctcttaata
     2761 ctattgtata ttgattgtac tattttttct ctagtgatag ctcacttaat gctacctctt
     2821 ctgtaattgt agcatatctt tctttaaatc ttttacagat tcttttctat ttttatatga
     2881 tacataattt aaagtatttt taatttgatg cacaacatat ctttgtactt aagtatcagg
     2941 aaatatcgct ttaattgcat tagaaaattc aactagtcta tctatagaag ctgctattaa
     3001 tacatcttta attcccatgt ttctagcatc tataagcact tttagccaat actttgaaat
     3061 ttcattttct ccaatccata ttcttaaaac ctcttttatg ctacatatag ctatatagac
     3121 tgttttgttt attattcgac ctcacttcta actttataat gtatagcatc tataaataca
     3181 attgaataaa ttatattaag aggtctaagt taccatttat gagttaaagg aataacttta
     3241 tcagtagttt tagttattag tgaatgagat aatatcaatt ccataagtac ttttaagatg
     3301 acttaatata ttttgagtag acatgccttt tgagtatatt ccaatcactt gattttttat
     3361 tcctaatata tcattttcta tcataaccta aaagttcatt aagctccgct tctaacattt
     3421 aatggattgt actttcaaat aaatctttta gtatatcttg aatatctgta attgttttgg
     3481 catcatattc ttcaataaat ttttttaata ttttagtttt tgttaaacat aaaaagtgaa
     3541 catcttcctt tgtatagtag tgatatttta attatcacaa ctttttagaa gaatacaaac
     3601 tttattttaa agacactgaa aatggtgttt ttataccttt atttgctatg tataatttta
     3661 cttataacat aaacttatat a
//
LOCUS       DACTGW010000285         3669 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.288, whole genome shotgun sequence.
ACCESSION   DACTGW010000285 DACTGW010000000
VERSION     DACTGW010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.288"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            243..620
                     /locus_tag="I9573_003403"
     CDS             243..620
                     /locus_tag="I9573_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT4797988.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDATVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLNAQKIDRYTHYTVIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDTLDFLESYFDNLKNE"
     gene            613..1647
                     /locus_tag="I9573_003404"
     CDS             613..1647
                     /locus_tag="I9573_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HAT4797989.1"
                     /translation="MNNYMLYVFDNLIKTTIVCSFGICLLLFLKRYFFKRFSKRFNYY
                     IWLVIVFRMLLFLFNYTVMYEVKEPKENTVGNNITQIDVSIDNDFMLYLVYLWLFVTI
                     AIAVYTFMEYTKFKNLVVDVSYDIEDHDVNCIYKNLLKELDIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYTYSKDELTWILKHELMHFKNKDILIKFLVLSVKIMYWFNPLVYI
                     MSNKVNLDCELCCDESVLTDCSLKDKKAYALALIKSIKLSKNYNSGILTTEFNKTNLE
                     KRLDSIIKKKGKSGILMLLILFTLFSVTYFNFESISFDNSNYLRNIGILEKTGADLKE
                     EDDISMRVVN"
     gene            2126..2299
                     /locus_tag="I9573_003405"
     CDS             2126..2299
                     /locus_tag="I9573_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAT4797990.1"
                     /translation="MHLVQLLCQQINGSDIDPDSKFGPATYNQVKILQGRLGVTKDGV
                     VGKATWQAVGERY"
     gene            complement(2384..3406)
                     /locus_tag="I9573_003406"
     CDS             complement(2384..3406)
                     /locus_tag="I9573_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HAT4797991.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTLLNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNSGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTKLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
BASE COUNT         1429 a          474 c          509 g         1257 t
ORIGIN      
        1 gtgaaaatat aggaattttt attaaataat tttaatgtgg aaataacatt ctgcactatt
       61 ataattaaat atgtaaaaag caaaatactt gaacaactat attttaaaat ctaactttta
      121 gtattttaaa aaattagaaa attaataaaa agtacaaaaa tccacatttt ttcataaagc
      181 gtttgactaa aaaattcaca tattgtagac ttaaagtagc aagagttttg agggaggtgt
      241 aaatgatgat aagaaaaata ccacaagcag aactaaaagt aatgaaattt atttggaaag
      301 tagatgctac agtgacatca aaagatgtta ttgaagttat ggaacaaaaa tatggttgga
      361 aacaaaccac tacactaaca cttttatcga ggttagtaaa aagaggattc ttaaatgctc
      421 aaaaaataga cagatataca cattacacag taatagtaaa acacaaagat tatttaagtt
      481 ttgagacaaa ggatttccta agtaatatac atgatgattc tttgaaaagc ttaatttcag
      541 cattacatga tgatgaaaat ataggtaaag acacgctaga ttttttagaa agttattttg
      601 ataatttaaa gaatgaataa ttatatgcta tatgtttttg acaatcttat aaagacaact
      661 attgtatgta gttttggtat ttgtctatta ttatttttga aaagatattt ttttaagagg
      721 tttagtaaga gatttaatta ttatatttgg ttggtaatag tctttagaat gttacttttt
      781 ctatttaatt atacagtaat gtatgaagta aaagagccca aggaaaatac agtaggaaat
      841 aatattacac aaattgatgt ttctatagat aatgatttta tgctgtattt agtttattta
      901 tggttatttg taactatagc cattgcagtt tatacattta tggaatacac aaaatttaaa
      961 aatctcgttg ttgatgtatc ttatgatatt gaagatcatg atgtaaattg tatctataaa
     1021 aatttgttaa aagaattaga cataaaaaag aaaattgagt taagaggttc tgatgaattg
     1081 ataagtccag caggtatggg attatttaaa tcttacatat tcttaccaga ctatacttat
     1141 agtaaagatg agttaacttg gattttgaaa catgaattaa tgcattttaa aaacaaagat
     1201 attttaataa agtttttagt tttgtctgtt aaaataatgt attggtttaa tccacttgtt
     1261 tatataatga gcaataaagt aaatctagat tgtgaattat gttgtgatga aagtgttttg
     1321 actgattgct ctttaaaaga taagaaagca tatgcgttag cacttataaa gtcaattaaa
     1381 cttagtaaga attataatag tggtatttta acaactgaat ttaataagac aaatttggaa
     1441 aagagacttg atagtattat taagaaaaaa gggaaaagtg gaatattgat gttattgatt
     1501 ttgtttacat tattttcagt tacatatttt aattttgaaa gtattagttt tgataattca
     1561 aattatttaa gaaatatagg gattttagaa aaaacaggcg cagatcttaa ggaagaagat
     1621 gatattagca tgagagttgt aaattaatag atttttatag tattataatt tattaacaaa
     1681 ttaggagaag ttttgaaatt aaaaatctaa aaaaaatctt agtagtcaca gggagggtga
     1741 ctctaatatc tattagttta gtatttgcaa atactaataa tgatgtaaat gtatgaaaaa
     1801 gtaatgaaga gcaaacaaca aattcattta caataactgg ttacgaattt atttatgaaa
     1861 atgtaccaca acctataaaa tctgattatg aaaaaaatgt aaagaattaa atataaatcc
     1921 atctaaagat gatacaatct ttattaatga aaaatttata gattcatata atattaatat
     1981 agatgattat aatattgaac caaaagcatt tattattaga tataaaaaag atttaaatcg
     2041 ttttgatgct actggtccta taagttatag cgtaaatata aataccactg tagtaggata
     2101 tggacatgta gaaaaaggga atcctgtgca tttagtacaa cttttatgtc aacaaataaa
     2161 tggttctgat attgatccag atagtaaatt tggacctgct acatataatc aagtaaaaat
     2221 acttcaagga agacttggtg taacaaaaga tggtgttgtt ggaaaagcca cttggcaagc
     2281 tgtcggagag cgttattaga aaagaggttg tcgcactaag aaatagggcg acaacttttt
     2341 tatatagaat atattaatca gtatcttaga gtctagaaat attttacaat aaattccata
     2401 taactctacc atctatatcc ttaacatttt gacaaccagt caaaatcata gtctcacata
     2461 actcattcct aaccttctct ataaaaaact ccacaccatc aagaccaccg ccaaaagaag
     2521 cagtaacaaa aggtcttccc atcaaaacag catcagcacc caatccaagc atcttaacta
     2581 catcaacacc agtcctaact ccaccatcaa caagtattgt tatctttccc tttacagact
     2641 tagctatatc tggaagtacc tcacaagtac caggagtata atcaagtact cttccaccat
     2701 gatttgaaac aataatggca ctagcaccag actctacagc catcaaagca tcatctacag
     2761 tcataattcc ttttataata aaaggtaatt tagtagattc aaccaattct ctaatctcat
     2821 caattgtctt aggagaaaaa ggattttctt gaaattgatt attaataagt ccacaagcat
     2881 caaggtctac accaactgca aaagcacctg cttcttcaga caatctaatt ttttctatta
     2941 tctttgaatt attccaaggc tttataaata caattccatt tccactatta tctttcaaca
     3001 catctaaatt atccagtaaa aaagtatcta cattagtatc gccaaccata gcataaattc
     3061 cagaattaga acaacctcta acaacaggct ctatgtattc tttttcggaa actttaccac
     3121 ccatgttcaa tagagtacct gatacaggtg cagcgaatat aggagaactc atttttctac
     3181 caaataattc tatggatgta tctggctttg aaacattatg tataactctc atatttatct
     3241 ttattttttc tagacttttt ctattttcaa tgaaagacga accacttcct ttaccaccca
     3301 taccaggaac atctccagca caagctaatc cattacaaat tttacatact ttacatgttc
     3361 cattaaagtt ctctctagct gattttagta agtcattgta attcatacaa aatctctcct
     3421 ttttataata ttaaaattga gtattactat attgaaaata tcttaattgt aaatataggg
     3481 atgttattat gatgagctaa actagataat acatattaga tattttttat tgttgtagat
     3541 agttaaatta tatcatatta gatagtcaaa ttttgaaaaa aaactaactt atttaagtat
     3601 acattttgaa aaaattaaaa aagctatata tagattttta aagaaatcta tatatagctt
     3661 ttaagttta
//
LOCUS       DACTGW010000286         3634 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.289, whole genome shotgun sequence.
ACCESSION   DACTGW010000286 DACTGW010000000
VERSION     DACTGW010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.289"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            122..775
                     /locus_tag="I9573_003407"
     CDS             122..775
                     /locus_tag="I9573_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAT4797992.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNSFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDEIRGAIFDVSQAIDLTKLMGIIIK"
     gene            916..2187
                     /gene="brnQ"
                     /locus_tag="I9573_003408"
     CDS             916..2187
                     /gene="brnQ"
                     /locus_tag="I9573_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAT4797993.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2389..3576
                     /locus_tag="I9573_003409"
     CDS             2389..3576
                     /locus_tag="I9573_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HAT4797994.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMTKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
BASE COUNT         1343 a          398 c          668 g         1225 t
ORIGIN      
        1 ataaaagtac tgaatacacg gatatttcgt aaaaactttt taatatatca aatatgtata
       61 ttaagttaat atattaaaaa aaataatatc aaaaataata taatttatag tggaggggaa
      121 aatgactaaa attattaaag tgcaaaaacc taaatttaca ggagaattag aaattattga
      181 aaatcttgaa gatatacttg aagatatatt taatgatgaa aaaatattta ataaaattat
      241 agaaaatgaa gttataagtg atttagattc tgtaagagtt agctttgaat cttgtatatt
      301 tagaaatgta tcctttgaaa attgcaattt aaaaaagatt gatttacttg atgtggtatt
      361 tgaaaactgc aatttatcaa attcattttt tgatggagga agtatataca gagttgaatt
      421 taaaaatagt aaattgatag gagttagatt tgatgattgt atacttaaaa acgttttgtt
      481 caaggatgtt ttaggtaagt attctaactt ttcattctct aaatttaaga tagtttctat
      541 cttagattca aattttgaaa attctgtatt tcaagaagta aaaggtgaca atctagtatt
      601 tgagtataca aacttaaaaa aagcaaactt taatggaact aaactttctg gcatagattt
      661 taccactaat aatattgagg gaattgagat tggtatagat gagattagag gagcaatttt
      721 tgatgtgtca caagcaattg atttaacgaa attgatggga attatcataa aatgaaaaaa
      781 atattcataa aataaattgg gaaaacgtta acaatgtgtg aatttttacc agattgacat
      841 ttttctgaaa ataaagtaag attatcttaa tattcaaaat attttcaaag ggggtaattt
      901 agtgtgagta agcagatgag agatattttt atagttggtt ttgctttatt ttctatgttc
      961 tttggtgcag gaaatcttat gtttcctcca tttttaggga tggaatccgg caaagactgg
     1021 ttaattcctc ttattggatt tgttgttgca gatgctggta tagcaatgct tgttattata
     1081 gcaacagcaa gatgcaaggg aagcatggat gatgtactta ggagagctgg taaagggtta
     1141 gcaagagtaa tgagtgtagc tgctctagca tgtttagcac ttcttgtaat accaagaact
     1201 tgtgcaacaa catatgaaat gggtattatg cctatatttg gagaccatgg accagtagct
     1261 aaagctatat tttcaataat cttctttggg ttaactttag catttactat taggtcttct
     1321 aaggttatag acataatagg taagtattta actccagcgt tattattagt attatttgtt
     1381 ttaatagtaa agggtataat ttctccagca ggacctatga gtcctgagca tatgatagat
     1441 aaaaatctat ttggtgaagg tatatcacaa ggttatcaaa caatggacgc tctaggtgca
     1501 gcatctatgg caactataat gttgatgtct ataatagcta aaggatatac atcagataaa
     1561 gaccaaatag gaatgactat taaagctggt tttgtagcct gtgtatttct agcagtagta
     1621 tatggtggtc ttacttattt aggtgcaaca gtttcaactt tatatgatac tagtatacct
     1681 caagcatctt tattagttga aataactagt atattgttat atggtcaagt aggaaaggta
     1741 attttaggtg taattgtggc acttgcctgt ctgactactt ctacaggttt aacagcatca
     1801 atatcaactt attttgaagg aataagtaag gcgagttata aacaactggt tataggtata
     1861 tgtgtagcta gtatgcttat atcaaattta ggtgttgatt ctataatagc tatatcagtt
     1921 ccaatcctac aaactatata tcctgtttta ttagcaatag ttgttatgga gttggctggt
     1981 aaacatataa agaatgataa tgcttttaaa ggtgcagcat atgttacatt agtgattagt
     2041 ctattatcag ctataaatgg aatgacagga gctgttccat ttatacaaag cttgccactc
     2101 gcaggtttgg gctttaactg ggtaatacca gctatattgg gtggaatagt tggtaacttt
     2161 ataaaaagca acaaacaagt agcataataa tttgtaatta atttaatttt ataatatatt
     2221 aatttttagt actatgggta ggtttaatct aaacctaatc tacccatagt actttttaga
     2281 ttggattgga atagcatgta tataattttt tataatataa tttttctttg aagtatgata
     2341 aaattagtaa tagtgataag aaagtacaaa tttaggaggt tttaattaat gcttagaaaa
     2401 ataacagcat ttatgctatc catatgttta attattggac ttacaggttg tagtttactt
     2461 gtaggcaaga aagataaaga gaaaattata gatgatttta aagttgcagt ggtaactcaa
     2521 ccattatctg aaaataaagt acaatacaat atggtggaag agatgacaaa agaatatgag
     2581 gaagagaata aaattgataa agacaaagat ggtcaaacta aagttaaaca aactataaaa
     2641 catgttgtat tgccagagaa ttttacttct aatatagata gtgctattaa taagatagta
     2701 aagttggctg acgataaaga agttcaggct attgtagttt caactgacca agcagggctt
     2761 ttacctgctt tacaaaaggt aaaggaaaag cgtccagaaa taataactat ttctgcccct
     2821 atgggagatg ataaaaacca attaagccaa tttgttgatg tgaatctagg ggttagtgca
     2881 gaggaaagag gaaaagtatt agctgaacgt tcaaaagaaa tgggagcaaa agcttttatt
     2941 cattatgcct ctacagatga tttgaaagat gttaatatag ccaaaagatt agaaatgatt
     3001 aaagagactt gtaaaaatat aggattgcca tttgtacaag ttaatactcc aaatataaat
     3061 actgaagaag acaaaaacaa agttaagcag tttttaaatg aagatattga aaaacaagtt
     3121 aaaaaatatg gaaaagatat aaatgtattt ggtgtaaatg agtatatgga tgaagttata
     3181 ttgactaagg ctttagaatt aaagtatata gttgcagagc aaagcaatcc atcaccaata
     3241 caaacgtatc ctagtgttat gggtctaaaa atatctgaaa aagatgctca aaattatgat
     3301 aaaataaacg acatgataag tgaaaaggct aaggcttttg gtatgtcaaa tagacttggt
     3361 ggatatccta tgccaatgga tgcttttcta ccatcagtag caatttatct tgctactgaa
     3421 atggtgaaac aagatttaac acaagaagat gtatgtgacc cagattattt agaggcgttt
     3481 acagaattaa gatttggaat cggttcagaa tttactcctt taacagaggt actttataat
     3541 tatcaatcag taattttaag ccaattaatt tattaatatt aaggaagtgt ctcaaaatga
     3601 actttttagt ttatgaggca ctatttttct gtta
//
LOCUS       DACTGW010000287         3589 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.290, whole genome shotgun sequence.
ACCESSION   DACTGW010000287 DACTGW010000000
VERSION     DACTGW010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.290"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            190..924
                     /locus_tag="I9573_003410"
     CDS             190..924
                     /locus_tag="I9573_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4797995.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            934..2067
                     /gene="nagA"
                     /locus_tag="I9573_003411"
     CDS             934..2067
                     /gene="nagA"
                     /locus_tag="I9573_003411"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAT4797996.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNITHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            2166..2915
                     /gene="nagB"
                     /locus_tag="I9573_003412"
     CDS             2166..2915
                     /gene="nagB"
                     /locus_tag="I9573_003412"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAT4797997.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKLENTHLP
                     NGMADDIEKECLNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            3090..3362
                     /locus_tag="I9573_003413"
     CDS             3090..3362
                     /locus_tag="I9573_003413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797998.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPIGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
BASE COUNT         1469 a          387 c          609 g         1124 t
ORIGIN      
        1 atatagttat ttatgtattt ttcaattctc tagcttctta tatagtataa actccaattt
       61 cttttttgtc tttaggtttt actaaaattt ttacaaaata agttaaaaag tattgaaata
      121 aaatttcttt taaagtataa ttggtatata ccaaaattgg tatataccat atcgaaaagt
      181 ggtgactata tgaaaatagt aaataaatct agtaatatac cattgcatac tcaattaagt
      241 tcaataataa gagagatgat agaaactggt gagcttaaag agggtgatgc aataatgcca
      301 gaaagagaac tttgcaacat acaaaatgta agtcgtatga cagtaaataa aaccattgtt
      361 ggattagtca cagaaggact tctttatagg gtacaaggta aagggacatt cgttgcaaag
      421 caaaaaaaga agtatcagtt ttcaaatgta aaaggtttta ctgatgttat gaaagaaaag
      481 ggagtaaaca taaaaacgga tatattatcc tttgagatgg aactaccaga tgatttagtt
      541 aaaagaaagc ttggaataag tgataatact acaaatatat ataagattgt cagacttaga
      601 tatactgatg gagagccatt tggattagaa atagtatatc tatcagaaga aatgtgtaaa
      661 ggtctaacaa aagggatatt ggataattct tcattatata gggttttaaa tgaaaaatat
      721 ggctataaga tacaaaaagc tgaacaagta atggagccag taatactttc agatgaagaa
      781 agtaagcttc ttgaaacaga tgaaggtgct ttagctctta aacttcatag aaattcctat
      841 aatagagaag gtagccctat agaatatacc atatcaatat ttagaacaga taaatatcaa
      901 tatgaaatag tattgagtga gtaggtgaat aatatgaaat gtttaattaa tggaaagata
      961 atattaaaaa atcaaatctt ggaaaataaa gtattagtat ttgatgaaaa aataattgat
     1021 atagctgata gtgtaccaaa agattgtgaa gttatagatg ctgatggaaa atatatatct
     1081 ccagggctta ttgatattca tatacatgga aatatgggaa aagatacaat ggattcgact
     1141 gatgaatcta ttgaaacaat ttcaaaatca attatgagac atggagtaac atcttttctt
     1201 ccaactacaa tgactatgga taaagaacat gtttatgatg cacttgaagt tattaaaaaa
     1261 gcccaaaata gaaagcttga aggggcacaa gtattaggag cacatttaga aggacctttt
     1321 ataaatgaga attataaggg agcacaaaat gaaaaattta taataaattc aaaatacgaa
     1381 tttataaaag aatataaaga tgttataaaa gttataactt atgctccaga aaaagatatt
     1441 gattttgatt ttactagaga aataaaaaga tgtacagata tagttttatc tattggtcat
     1501 agtaatgcaa attatgacca agctaaagaa gctataaatt taggagtaac taatataact
     1561 cacatgttca atgcaatgac tggtttaaac catagagacc caggcgttgt tggtgcagca
     1621 cttacaacta atgtgtacag tgagctaata gcagatacaa tccatataaa taaagattta
     1681 tttcaattta tactaaataa taaagggaaa gaaagactga ttttaataac agattctata
     1741 gaagctggtg gtcttgaaga tggcaattat tctcttggag gtcagaaagt catagttaag
     1801 ggtaatgaag ctagacttga aaatggagca ttagctggaa gtgtgttgtc tttaaataaa
     1861 atggtattta acttcttaga taatacaaac cttaaagtga atgaagcaat taatttagct
     1921 tctttaaatc cagcaacatc tttgggaata aatgacaaaa aaggaagctt agaaataggt
     1981 aaggatgcag atatagctgt atttgatgag aatttagatt gtaaaatgac actttgcttg
     2041 ggggacgtag tatataaaaa tatatagtat acaaaacaaa ctttaaagac agtaaaaaca
     2101 atataaaaaa taataaatta aacttacatt agttaaagta gtagttaaaa tttaggagga
     2161 ataagatgag agtattagtg tgtaaagatt atgatggaat gagtaaaaaa gccgctgaaa
     2221 tgatagcagc tcaaatagtt ttaaaaccaa attcaatact gggactagct acaggaagta
     2281 caccagtagg tatgtataga gatttagtaa aaaaatacaa tgataatata gtagattttt
     2341 cagatgtgat gagctttaat ttagatgaat attataagtt accaatatct aatgaccaaa
     2401 gctatgatta ttttatgaaa gagaatctat ttaatcatgt aaacataaag ctagaaaata
     2461 ctcatctacc aaatggaatg gctgatgata ttgaaaaaga gtgtctgaac tatgaagctt
     2521 ctatagatgc agctggtggt atagatgtac aagtacttgg aataggtcgt aatgctcata
     2581 taggttttaa tgaacctgat actaaatttg caaaaagaac tcatgtagtt gaacttacag
     2641 agtctacaat agaagctaat gcaagattct ttaaatcaag agaagatgtt cctaaaaaag
     2701 ctgtaagtat gggaataggt tcaatattga agtctaagaa aatattgctt ttagcttcag
     2761 gagaagaaaa agctgatgca gtatataata ctgtttacgg agatataact ccagaagttc
     2821 ctggttctat acttcaactt cataaagaca ctatagttat agtagatgaa gcagcagcaa
     2881 gtaaactgaa tccaaaagat tataaattag tataagaata gaatgtagtt taagtgaaaa
     2941 atagctatca ttgaataatc aaatttattt aatgatagct atttttattt aaatttttta
     3001 cttcttttta tttaaattag ttattattgt ttaaattact tgcttattac tttttacgaa
     3061 aactcgttat caagaagtat ccgaataaga tgttaaaaat tattatgttt agtgggattc
     3121 tatcaagcat aataaaatta cctataattg ggatattacc aattggaaac ataaagaaga
     3181 atacaggcat cagtgatagt aaaaaagatg gaaaacaaag aagaagtaat cttggaatat
     3241 caatatacca actaccatcc tttttgtatt ctctaatcaa taattcagtt cctagtaaca
     3301 aaccaatacc accagaggaa attaaattaa taaaaatgta tcttttaaag tcttgcatat
     3361 aaccagtgtg aaattgagta tagaaatcat aatttaattt aacggctaaa aaatatataa
     3421 gcaatagaat gattaaaaat aaaagatatc taagagcttt tttcatttta aattctccct
     3481 tcagaaatac tttattcagt tttattttgt tggaatctga ttttatcatt tagtttaaga
     3541 aatccataat attagtgaag aatgaaataa gtattatata tgaataaat
//
LOCUS       DACTGW010000288         3552 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.291, whole genome shotgun sequence.
ACCESSION   DACTGW010000288 DACTGW010000000
VERSION     DACTGW010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.291"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..1851)
                     /locus_tag="I9573_003414"
     CDS             complement(<1..1851)
                     /locus_tag="I9573_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4797999.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCNMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAKDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNQLDIDVEWDFTDYYEKLEKGENFDITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKTIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNSDEKQPNTPKDD
                     VESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNPITENDDNYDLSSKSVFGTNEH
                     SFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFC"
     gene            complement(2035..3510)
                     /gene="spoIVA"
                     /locus_tag="I9573_003415"
     CDS             complement(2035..3510)
                     /gene="spoIVA"
                     /locus_tag="I9573_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HAT4798000.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
BASE COUNT         1032 a          695 c          320 g         1505 t
ORIGIN      
        1 acaaaataaa aaaataatat ctttttttag attatattct ttagattcta atttataatt
       61 attatttatt attccatcat aatcacttat atttgactta tttttatttg tatcttgatt
      121 tttgttagaa atactttccc tataaggaaa agaatgttca ttggtaccaa atactgattt
      181 tgagcttaaa tcataattat catcattttc tgtaattgga tttttattgt aaaatccttt
      241 ttctttatca gaattatttt ctttattacc gtctttaggt atatcatgag gatttttatt
      301 ttcattgcta tcagtacttg attctacatc atctttagga gtatttggtt gtttctcatc
      361 agaattatta ttttcattat tattgttttg attgttatta ctatcattat tttcatttcc
      421 attattccca tcttgattgt tatcatcctc atctctatcc gggtcgtctc tacctcctcc
      481 tccacggtca ccatcaatat catcattttc ccgttcatca tctggcgtct cttggttgtc
      541 tccgcctccc attataaatc ctggtttaaa aactatattt gaataccctt cttttaatcc
      601 tcctaccact tttactctaa aacaacgcaa cttaaaatct tcaaaaaaaa gtatagcttg
      661 atctctaata tcctcctgtt cttcagatat ccaattgatt ccatcgcttg aatattcaac
      721 aaaaacctta cttgcactat acggcatatc ataaaataaa acagctacat atccattttt
      781 tgtattttga aattccagtt ccaaattgtc tattgttttt ttctctataa cagatacatt
      841 caaaatagga actacatcat catttattat aattttttct ttatttaaca tttctgtttt
      901 aaactttcct gttatatcaa aattttctcc cttttccaac ttttcataat aatcagtaaa
      961 atcccattct acatctatat caagttgatt accactagat gtttctaata ccatatattt
     1021 gggaaatttt atattttcta ttttatcatc tttacttact tttatttcat cactaaatac
     1081 accaattccc ctatgacaac atttcacaat attatagcca gaatctttag ctatagaggg
     1141 tataagctca ttataatcat caattatata aactttctta ctttctgata taattgattg
     1201 tgccaactta ttattatctc ttagtaaccc actttcattg ttgctactat atgcttttat
     1261 tgaagtttct tctatctcaa catcaccttt agaataaaca cctatagcat gttctccatt
     1321 cacttcaata tccttattta gtattctaat atcattttca gtataaattc ctattgcatc
     1381 ttttccattt gcctttattt tttcaacaga ttcgtaggat gatttaatat gtaagtctcc
     1441 tccttcttca acgtataagg ctgtcccatc agtagcaatt atgttgttta taccaatact
     1501 tatacttcca ccattttcta tatgtattac tccttctttt cctccttcac ctattatatt
     1561 caattcatta tcatcaataa taaatctccc atgatttttt atcaatattt tatatttgtt
     1621 ggtatctatt gtaaaattac tgtcataagg aatataaaat ctaaactctt catctattac
     1681 catattacaa gaaagagtat aaactatttc ttctttatta tttttatttt cttgaaccca
     1741 cctttcaaaa tcttcaagac aattttcctc ttgtccaaaa actttatgtg gaaaacttat
     1801 aaaacacaaa actattatac tcataaaaat aaatatctca tactttttca tcttacactc
     1861 ctagaaattt tttagctttg aaacaatcct gtcgaaacat atacttcacc cactataatt
     1921 tttccacaat tatcaattat tgtcaattta tataagtatc tttattaatt gttataaata
     1981 aaaagctact tactaaagta aacagacgtt tcactttaat aagtagctat atttttataa
     2041 caaaatagtt ataatattag aacttccttc atttacaatt ttttgtaaag tcttttgcat
     2101 tttaactctt atctcttctg gcatagtata taatttactt tgtaattgct cttttactaa
     2161 gtcatgtagt gacttaccaa acatatttga ttcccataaa tctgctggtt gctcttcaaa
     2221 tacttccata agatatttta tcatctcttc accttgattt tgatttccaa ctattggaga
     2281 gacctctgta gatatatctg cttttataat atgaagtgat ggtgcattgg cttttaactt
     2341 gataccatat tgttttcctt gtttcattat ttctggttct tccagactaa gttcttctaa
     2401 agaaggagct actactccat atccttttat cttggcatct attaaagcac tttctatttt
     2461 atcatactca tttttaactt tagatagtct agttatcaga ctaagcagtt gataatctcc
     2521 ttcaatttta aatccacttt tttcttcaag gacattatag aataaatctt gctttgtctg
     2581 taaatcaata tttataacac cttctcctag ctctacatta tcaacacctg tatcttccaa
     2641 aaactctaac tcagaaaatc cttgtataat accttctata tctcttattt tacctatatc
     2701 tataatactt tgttttaatg ttgttattat gctacttttt atccaatgat ttctttcaag
     2761 accttcaacc cattttggta agttgattct tatttctgta agtggaaaat catataatac
     2821 agcttccata acctcctcta tatcttcttc ttccatttca accacattca ttggaagaac
     2881 tggaacttca tacttttctt ccaattcact tctaagcata ctcgtttcct cactttttgg
     2941 acttaacgta tttaaaacca ctgcaaatgg ttttttaaga tttttcaatt cttgtataac
     3001 tctctcttct ggttctacat agctttttct gtcaattcca gtaactgaac catctgtcaa
     3061 gacaactatc cctattgtag aatggtcttt tataactttt ttagttccta tctctgctgc
     3121 cttttcaaat gtcatagctt cttgtgacca aggagtggat actaatcttt gttttccacc
     3181 ttcctcatgc cctaaagctc cttcaactat gtatccaaca caatctacca ttcttacttt
     3241 taaagatact gtgtctttta tttttatttc tacaccatct gctggtacaa attttggttc
     3301 tactgtcatt atcgttttac ctgaaccact ttggggtatc tcatctcttg ttctatcctt
     3361 tttaaattca ttgtctatat taggtataac caacttttcc ataaattttc ttataaaagt
     3421 tgattttcct gttcttacag gtccaactac tccaatatat atatccccct gagtcctttt
     3481 ggatatatct tcgtatatgt tattattcat attattcctc ctatacatct aatcttatta
     3541 aattatattt ag
//
LOCUS       DACTGW010000289         3548 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.292, whole genome shotgun sequence.
ACCESSION   DACTGW010000289 DACTGW010000000
VERSION     DACTGW010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.292"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            98..517
                     /locus_tag="I9573_003416"
     CDS             98..517
                     /locus_tag="I9573_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HAT4798001.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            648..2489
                     /locus_tag="I9573_003417"
     CDS             648..2489
                     /locus_tag="I9573_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT4798002.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFVENKIGVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSIEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNQSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYTIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKN
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIETEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMFMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIREIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            2516..>3548
                     /locus_tag="I9573_003418"
     CDS             2516..>3548
                     /locus_tag="I9573_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT4798003.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKEDSDIISKIYIYLT
                     LSAISIRKYDKANNFYFSAKKVIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSKNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELRKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNS"
BASE COUNT         1523 a          265 c          530 g         1230 t
ORIGIN      
        1 cataacttaa ttataaagat tataaacaat tacttttatt aactttattt aagtggtaga
       61 ataacttgga gattaataat tataaggagg atataatatg agtttataca agtttatata
      121 tgatgataag gaatatttat taaaagagga gaactgtagt gctttaataa atgatgaaga
      181 aaatccagtt caaggagtaa gtatttcaaa aataattgat atacttaatg aagcagaaga
      241 agttgatttt gatgtagaat attatcaaga agcatgtcca ttatgtttag aaggagtaaa
      301 agaaaagaaa aaattctttc cttttttaga atatcacttt tatattttca gtaaagatgg
      361 agaatatgta ataagcaata tatctgttga ttataaagga ctatctttta ataaactatc
      421 aagggcaaat aaagttgata atagttatat tgtaagtgtg ataatttgtg aaaattgtca
      481 ggattatata gttcagatag aaaattgtat agtttagtgt aaagtatgtc ttaatttaag
      541 agaaatttct aaattgagac atcaattttt ttcaaaaata gtatggaaaa ttactaaatt
      601 tatgagataa tgtaagtcgg aggtgagtta caatatatag taaatatttg gaagagatag
      661 atgatacaat tttagattca atgtttaaac taaccttgaa tgaaagatta ttgactaata
      721 acgattttgt tgaaaataaa attggtgtag taaagaattt aattgattgc tataaaactg
      781 aacaaaatag tttaatgtta ttaaaatcaa attttttgct agctttatta tatggtattc
      841 aagggtttag tgaaaaaatg aaagagtata ttttgatttc ctgtaaatat attgataaat
      901 gcttacccaa agattataaa tttttagcac gattttattc acaaatagct attttaagtt
      961 taaaaaatga agatgtaaat aaggcaaatt tatatatgga taaatttaat cttatttgtg
     1021 atgaaaataa ttttctaatt gaagaaatta tttttaaatc acaacttatt tacattaagg
     1081 caagtaagtg tatagaatct tctgtaataa ttaaagaaat agaaacattg tatataaaga
     1141 taaaagaaat taacgatttt aactgtaaaa aaatatattt ttttattatt ggtaaaatat
     1201 attttttgtt tttggatgat gctctaattg ctaaagtaaa ttttttaaaa gcaagaaaat
     1261 atgcagattt actaaaggat atgcaagtat cttcactatg taatataaag ataggtgagt
     1321 gtgaatgttc atttgaaaat tatgaatgtt ccatagaata tttcaatgaa gtaataagca
     1381 ataaaaagta tagaaatgta aatataattc aaaaatatag agcatcaaac aacataacta
     1441 aaatattaat aaagactaga aattatcctg tagcaataaa taatttagca aaatctgaag
     1501 tatatctaga aaaaattaaa aatcagagct taaaagaagt agagaagttt gatttattta
     1561 taagtttagc tatgtattat gcaaaaagta atgaaaaatc ttttgaacaa tcaatatgct
     1621 atttagatag agctaaaaac atcttaaata tgataaaaaa tatagaagaa tatacaatat
     1681 atgatttaga aataatttat catcaggcaa agatatatta tatctttgga tattatgaca
     1741 atgccttaaa cacaaataaa aaactccttg aaaaatctaa aaatgctaaa aattataatt
     1801 atgtgaagtt agcatacaag aatatatatt tgtgttttga aaagattcaa aattatgaaa
     1861 tgagtatgaa atactttaaa atgtattatg agttaaaaat gatgtatata aaagctcgaa
     1921 atagaaatta tatagattcc ttaaattata gacatgagta tatagaaaca gaaataaata
     1981 acatgagaaa agtaaaactt gatttagata aaaaaaaata tgttgaccat ttgacaagtg
     2041 catataatag aacttttttg aacgattttt tagaaaaaca agaagttagt gaatattgca
     2101 cagcatttat gatagatgta gattacttta aagaatacaa tgatacttat ggacactata
     2161 atggagatat tgctcttaaa aatataacta taataataaa aaaatatcta aaaaaagaca
     2221 tgtttatgat aaggtatggt ggagaagaat ttctgatatt atctgtatgt aaagattata
     2281 aaaaaagcaa aatatttgga agaaaattat gtaggattgt aaagaaattt ttaaacaata
     2341 atttgacagt tagtattggc attgatacat gtaaaaatag tagtatagat ataagagaaa
     2401 taattgaaaa tgctgataaa gctttatata aagcaaagga aagtggtaga aatagatgtt
     2461 tgcattatca tgattttaag aatttttaac tgttgaaata ggattggtga tttacttgaa
     2521 aaggagttta atagaagatt taaagagaaa tataaacaag atagaatacg atatgttgac
     2581 taaattctat ctaaatttga cagatttaaa ctacatcttg gattatcaca aagacctttt
     2641 aaatacaaaa ctagaagata atagtagagt ctatacttta tatttaatta gccttataaa
     2701 ctatataaaa aaagatagag aagcatcata caactatttg atagagtcat taaagtatat
     2761 taaaaaattg tcattaaaag aagattcaga tataatatct aaaatttata tttatcttac
     2821 tttatcagct atttctataa gaaaatatga taaggcaaat aatttttatt tctcagcaaa
     2881 aaaagttata agacaaaatc atttaaatga gttgctagtc ttgttaaata tgagtttatg
     2941 tgtagaaata agtgctttat atcagaccaa aacggatgta atagctttag tagaagaagt
     3001 gtcttttttt aaaactttaa aaaataaagc tcttgtaagt agggcaaatt atatatttgg
     3061 gagagtttat ctatatttat ttaataactt tataaaaagt atggagtacc taattgaatg
     3121 tctaaaactg gctcaagaaa ataatttata ttctatagag gctttttgta aatgtataat
     3181 aagtttgtgt tatttagaga gtggaaataa tgtagaagct ataaaataca taaataatgt
     3241 attagatgtt gcgcacagta agaactcaat atcagtaact gaaagagtgt ctataaagat
     3301 agaccttata tgggcatatt taaaagaaga tatgaataaa gaagcagaag ttattttatt
     3361 aaagactata aaattaatga aagttgttga aggtgtatat aaggattatt tatattcatt
     3421 tatattttta tattcagctg aaattgagtt aaggaaagat aattgtagtt ttgaaaaggt
     3481 aaatacctat ttaattttat caaaaaatat atataaaagt tttggcgatg tttttatata
     3541 taattcta
//
LOCUS       DACTGW010000290         3492 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.293, whole genome shotgun sequence.
ACCESSION   DACTGW010000290 DACTGW010000000
VERSION     DACTGW010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.293"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            238..969
                     /locus_tag="I9573_003419"
     CDS             238..969
                     /locus_tag="I9573_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAT4798004.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLYMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQNNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            1049..1684
                     /locus_tag="I9573_003420"
     CDS             1049..1684
                     /locus_tag="I9573_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAT4798005.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNIHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(1935..2528)
                     /locus_tag="I9573_003421"
     CDS             complement(1935..2528)
                     /locus_tag="I9573_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HAT4798006.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(2599..3462)
                     /locus_tag="I9573_003422"
     CDS             complement(2599..3462)
                     /locus_tag="I9573_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAT4798007.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDDLYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
BASE COUNT         1250 a          516 c          374 g         1352 t
ORIGIN      
        1 gctctaaaaa catgagcttg cataaaatta cacttattat gactactaaa ttcgtaatat
       61 ttacaatgtt taaaatatat tataacacat atatgatttt atttttctac atttatataa
      121 tatataaatt acaaatagtt atttaactaa tatataacaa taccttttgg aatatgatat
      181 attatattaa ttaaattttt ataagataag ttatacttat aggagggaac catataaatg
      241 aatatttatg ctcatagggg gtttagtgga aagtatccag aaaatactat tctagctttt
      301 aaaaaatgtt tgtatatgga tatatatgga atagaacttg atgtccacag aactaaggat
      361 ggaaaaattg tagttattca tgatgaaaag gttgatagaa cttttaatgg acatggtttt
      421 gtaaaagact ttactttaag aaaattaaaa actctaaact catcttttga aggatatcaa
      481 aacaataaag aatgtaaaat tccaacttta gaagaagtat taattctaat ttcacctaca
      541 gaccttattt taaatataga actaaaaact gacaaaataa actatcccaa tatagaaaaa
      601 gacgttttag agcttatttt aaagtataat atgaaaaata gagttcttat atctagtttc
      661 aacagcaata gcttaaaaaa ctttcataaa ttagacccta gtgtaaaaac aggattattg
      721 tgttatttac ctataaacaa tgtagtcaat tttgctaaat ttctgggtaa tagttacctt
      781 catccacctc tagttttagt aaatgaatct ctaattgaac tttgtcacaa aaatctactt
      841 ggtgtcaatg tatatactgt aaatgaagag gatgatatac tacactgtct aaaactcaat
      901 gtagatggta tttttactaa ctatccagat attgcttcaa atcttttaca ctcaaaacaa
      961 tacagctaat tatacaatag tttgtactcc ttgactttta tataattaat atgtacaatt
     1021 ttattaaatt tatcttggag gtataactat ggataactta ttaaatgaac ttgaagcttt
     1081 aaagagtaat ttagataatc taccaataga aaaaatagaa aattatgatt tgagtaaaac
     1141 tgctttattt ataattgatg ttaataatgg ctttgcaaga caaggtgcat tatactctcc
     1201 tagagtagag tctttaataa aaccaattga aatgtttaca aaaaaaatat caaacaaatt
     1261 aaatagagta atagcattta ctgattctca tactcctaaa tcaatagaac ttttgagtta
     1321 tcctgttcac tgtttggaaa atgatgtaga aagtgaattg gttgatgaac ttaagtcgat
     1381 agaaaaccta caaattcttc ctaaaaattc tactaatgga ttttttgcat tagaaaattt
     1441 ggattttgat aatatagata atataattat agttggcgat tgcacagata tatgcatata
     1501 tcaatttgca ataactctaa aatcatattt taatcaacat aatattgaaa aaaatatagt
     1561 tgtacctatg aatttagtag atacttatga tattcctaac attcaccctg ctgagatttt
     1621 aaacttagta tttttcaaca gtatgattca aaatggagtt aatgtattaa aagagattcg
     1681 ttaagcctct aatactttac atcccatata atacaattat cagatattta tttttaataa
     1741 aatttctaat agaaaaacac agttttagtg agaagattac aatttaaaac actatctatt
     1801 atgactaatc ttcacaatat aaaaaatcgc agtttagata ttccttatac aaatttataa
     1861 ggaataataa attgcgattt ttatttttaa gtagtacaat taattgatac tacttcagta
     1921 tttaaattca ttttttaata atcatactta ttcgtagttt cgcacattac tacctttatt
     1981 tttttattaa acatattctt atattctaat aaaaactcat ttagttctga atccataaga
     2041 tttatttttc tatcactaca aagcattatc ttttcttggt cgattattac attaatatca
     2101 tttaagtcat tttttataaa tgttgttata caactctttg gttggatttt ttctccatat
     2161 ttctcataaa atcctgcact cttcttaaat gactctaaat cttctgtaga tactttgctg
     2221 gaagtattat tactcataaa acctgctgta gaagggtcga ttccaaagtt tctaagttgt
     2281 ttttctactt cttcagtgtc cataccatat ctctccataa attgcttttg tattttcata
     2341 aatttttctt gagatatgtt gttatctctc atataatcga atatttttct tgaaaactca
     2401 gccatggaca tctttccatc cataacagaa aaatataagt tatatatgta ttgctcaaat
     2461 ttatctacat cagaatcttt aactttgttc tttagttcgt taaagtctat tattctatca
     2521 tcagacattt ttcatcctcc tagctaaatt tttacttgta taagattata ccatatttct
     2581 attatattta tatacacact atttttttaa tttaacgatt ttttctgtag catctaattc
     2641 gcacattctg cccaaaggaa tagttatcat tgggatacaa tgtcctgact gaagattata
     2701 tatagaaggt ttattgacct tatttgcaaa ttctattagt aattccatca aatcattttc
     2761 ttcaatacaa tccttaaaat ctccaaaaat tataccatta cattcctcaa gttttcctga
     2821 agaatatagt tgtgttaaca ttctatccag tttatattga ggttctccaa tttcctcaat
     2881 aaacaaaatt ttatcttttg catctatttc atattttgtt cccattgtgg caattattaa
     2941 ggataaattt cctcccatta ttattccctc tgctttacct tcacatacag tataaatctt
     3001 ttcttctttt ggattttcta aatataagtc atctttaaat tctaaagcat ttaccaatga
     3061 tttataggta aatttatccc atttacctac atttgctgcc attattccat gatatggtaa
     3121 aagtccacaa tttttataaa aagctaaatt taaacctgtt atatcagaaa aacctataaa
     3181 tggttttgga ttttgcttta ttatatcata atttatttta tccaatattc ttgttgttcc
     3241 ataacctcct cttaagcaca taattatatc tacatcttta tctaaaaaca tatcttctat
     3301 atcccttgct ctaatatcat cattaccagc taaataacct ttataattta aactacaact
     3361 ttcagaaaac ttaacttcgt aaccataact ttctaaatta aactttattt cctccaaact
     3421 tgtatttctt aatggcccag atggcgcaat tattcctaac atttaaacac ctcttcttat
     3481 aaaatgatat at
//
LOCUS       DACTGW010000291         3483 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.294, whole genome shotgun sequence.
ACCESSION   DACTGW010000291 DACTGW010000000
VERSION     DACTGW010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.294"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1509
                     /locus_tag="I9573_003423"
     CDS             <1..1509
                     /locus_tag="I9573_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT4798008.1"
                     /translation="KIIFIIIGILGIVLLTRHIEVDIKVLILILLTLFSINVFISYLK
                     IKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCVMACFDLCRLKLIN
                     DIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLENREQKIPYLVNLIK
                     NKIMILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMARLKSKGLKHIEYVE
                     FDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIGSEALIRWLHPSLG
                     MVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPVSINLSRVELYKND
                     LVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSLGMNISLDDFGVGN
                     SNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIINLSHDLNVTVICEGVEDMHQ
                     VKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDKCMTVEEE"
     gene            1512..1910
                     /locus_tag="I9573_003424"
     CDS             1512..1910
                     /locus_tag="I9573_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAT4798009.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            1950..>3483
                     /locus_tag="I9573_003425"
     CDS             1950..>3483
                     /locus_tag="I9573_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAT4798010.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGVGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKKILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAK"
BASE COUNT         1458 a          291 c          579 g         1155 t
ORIGIN      
        1 aaaataattt ttattataat tggaatttta ggaatagttt tacttacaag acatattgag
       61 gtagatatta aagttttaat tttaatttta ttaactttgt ttagtataaa tgtatttatt
      121 tcatatctta aaattaagtt gtatgaggat aaaattaatt taggattgat aaataattca
      181 gataagttta taaatattac taaagatgag aacttcctaa aacatgttca aaattatatt
      241 atcagtaatg aaaaagaaaa ctgtgtaatg gcttgttttg atttatgtag actcaaactt
      301 ataaatgata tatatggtta tgagctaggg gatgaagtgt taaataatat tttaagtaac
      361 ttaaaaaatt attttggaaa agaagctatt tatggaaagt taaaaagtga tgtttttgta
      421 ttaataataa agcttgaaaa tagggaacag aagattcctt atttagtaaa tcttatcaag
      481 aataaaatta tgatattaag tcagattgac agttttaaaa tggatataaa tattgaggct
      541 tctattggaa tttataaatt taacaataat gaaattgata taaaaaaagc tatagataat
      601 gctgatatgg caaggcttaa atcaaaagga ctaaagcaca tagagtatgt agaatttgat
      661 aatgctatgg aagaagaaat acaatcaatt atgaaaatag agagagattt attcttggct
      721 attaaaaata aagagtttgt aattcactat caacctaaag ttgattcaag tacaggtaac
      781 attataggtt cagaggcatt gataagatgg ttgcacccat cattaggcat ggttggacca
      841 aataagttta tacctatagc tgaaaaaaat ggtctgataa acaatattgg tagatggcta
      901 attcaagaag tttttattac cattaacaag tggatttcag aaggtataaa tgtcttacca
      961 gtctcaataa atctttctag agtagaacta tataaaaatg atttagttga tttttttaaa
     1021 ctgatgttta atacatataa tattccaaaa gaacttatag aattagaaat tacagaaacg
     1081 acagcgctaa gagatgtaaa atttataagt gaaaggcttt atgagattaa atctttaggt
     1141 atgaatatat cattagatga ttttggagta ggtaattcaa attttatcaa tcttaagggg
     1201 ataccactag atattataaa aattgatagg tcacttatat tggatatagt tacaaataca
     1261 aaaacaaagt ttatggtgaa agcaattatt aacctttcac atgatttaaa cgtaactgtt
     1321 atatgtgaag gggtagagga tatgcatcag gtcaaagtct taagtgaact gggatgtaat
     1381 ttcatacaag gttatgtatt ttttaaacct ttggatgaga tgaattataa aaaacttctt
     1441 actgacggaa gttatataaa tctgaaagat actcttttag ataaatgcat gacagttgag
     1501 gaggagtaag gatgatttca gaagatgtag attattcaat aagaatcata agagctatat
     1561 ttaataatgg agaagataaa ccaatagatg ctaaaatgat attaaaaagt gaagatatac
     1621 caatactaac actatacaga ctgcttagaa agctttgtgc aaacaatatt cttactttta
     1681 acaaagaaga tggcaagaca tacatgttaa atgtgtcatt ggatacaata agtctaagaa
     1741 ctattgtaga agtaattgat ggaaaaatat atataaataa atgtacgaga tatgtatcta
     1801 attgcagtaa ttgcttaaaa ggttgtaaag taaatcgtga attgctagaa atagaaaaag
     1861 aaatttataa acttttatcg agaaaatcta taagacaaat actagaataa aaaattttca
     1921 gctatgataa taattggagg ttttcggtta tggatgatat atctcaagat aattttctac
     1981 tgtctaaaga gtatgagaat agcttagatg tagatactaa aaaagcaagt ggaatatatt
     2041 atactcctaa aataattgta gattatattg taaaaaaaac tttaaaaaat cacgatataa
     2101 ttaaaaatcc atatccacga atacttgata tttcctgtgg gtgtggaaat tttcttttag
     2161 aagtatatga tattttatat gacttatttg aggaaaatat atatgaattg aaaaagaagt
     2221 atgatgaaaa ttattggact gttgataaca tacaccgtca tatattaaac tattgtatat
     2281 atggtgctga tattgatgaa aaagctataa gtatattaaa agatagctta acaaataaaa
     2341 aggtagtaaa tgatttagat gaatctgata taaagattaa tctattttgt tgtgatagtc
     2401 ttaaaaagaa atggcgatat aagtttgatt acatagtggg taatccaccc tatattggtc
     2461 ataaaaaact tgaaaagaaa tataaaaaat ttctcctaga aaaatatagt gaagtttata
     2521 aggataaagc tgacttatat ttttgttttt ataaaaaaat tatagatatt ttaaagcaag
     2581 gaggggttgg cagtgttata actccaagat atttcttaga aagtctatca ggtaaagatt
     2641 taagagaata tattaaatca aatgtcaatg ttcaagaaat agtggatttt ttaggtgcaa
     2701 atatatttaa aaacataggg gtatctagtt gtattctaac ttttgataag aaaaaaacaa
     2761 aagagacata tatagatgta tttaaaatca aaaatgagga tatatgtata aataaatttg
     2821 aaacattgga agaacttcta aagagtagca aatttgaaca ttttaatata aatcagagat
     2881 tgctatctga cgaatggata cttgtaaata aagatgatga aactttttat aataagatac
     2941 aagaaaaatg taaatatagc ttggaagata tagcaataag ttttcaagga ataattacag
     3001 ggtgtgataa agcgtttatt ttaagtaaag atgatgtaaa gttgaacctt gtagatgata
     3061 aatttttaaa atgttggata aaaagtaaga atataaataa atatatagta gataaaagtg
     3121 aatataggct aatatactca aatgatatag ataatgagaa tacaaataaa aagatactag
     3181 atgaaattat agggctatat aaaacaaaat tagaaaacag aagagaatgt aaaagtggaa
     3241 taaggaaatg gtatgaactt caatggggta gagaaaagct tttctttgaa agaaaaaaga
     3301 taatgtatcc ttataaatca aatgaaaata gatttgctat agattatgat aacaattttt
     3361 caagtgcaga tgtatattct ttttttataa aagaagagta tttagataaa ttttcatatg
     3421 aatatctagt tggaatttta aattccagtg tttatgataa atactttaaa ataactgcta
     3481 aaa
//
LOCUS       DACTGW010000292         3478 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.295, whole genome shotgun sequence.
ACCESSION   DACTGW010000292 DACTGW010000000
VERSION     DACTGW010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.295"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            306..953
                     /locus_tag="I9573_003426"
     CDS             306..953
                     /locus_tag="I9573_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HAT4798011.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTAFKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(1120..1689)
                     /locus_tag="I9573_003427"
     CDS             complement(1120..1689)
                     /locus_tag="I9573_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798012.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1694..2593)
                     /locus_tag="I9573_003428"
     CDS             complement(1694..2593)
                     /locus_tag="I9573_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAT4798013.1"
                     /translation="MKLLSKVNNNIRLIIQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2746..3330)
                     /locus_tag="I9573_003429"
     CDS             complement(2746..3330)
                     /locus_tag="I9573_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAT4798014.1"
                     /translation="MKKFFMVVLGCIFVLGVISGCSKKESKPVEKNKSNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKNIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEEWKKIIDERLKMVKANE"
BASE COUNT         1235 a          579 c          380 g         1284 t
ORIGIN      
        1 taaaatttaa acaagttatt cataaaataa aataatcatc atctataacc cacaacttta
       61 ttttatatta aataaaaagt attagtgatt atgttttata agtatttctt tattcagtta
      121 tttgttaaaa actaatagta aatcttatac taatcgcata tttatgttat aactatattg
      181 taactatttt cgtaatctta ggaaaagatt acttataaaa ttaaccttat acaataatat
      241 atcgtataat cttaattagc ataatagtta caatattaaa atttaaatat gggagtgatt
      301 ttagtatgat aagacaaaca ccattagaga aacgatatga caagctaggt cctcatattg
      361 taacagcatt caaaaaacgt tattttgatg cttattactg taaaactaga aatgaagctt
      421 tgcaacaaat tttagattta attcctcaaa atcatgtagt atcatgggga ggatctgaaa
      481 cacttaaaga aatgggcgta caaactgctg ttagagagaa aggatttaaa gttattgata
      541 gagatttagc aaaaactcca gaagaaagaa ctgaaattat gcgtcaagca cttctatgcg
      601 atactttttt gatgagtagc aatgcaatta gtgaagatgg acaactcttt aatattgatg
      661 gaaatggcaa tcgtgtagct gcaatgatat ttggtccaaa aaatgttata gttgtagctg
      721 gtatgaataa aattgtaaaa aacattgatg atgcaataca aagaactaga acaattgcat
      781 ctccagcagt tgttcagcgt tttcaagatg tcaatacacc atgctatatt aatggaagtt
      841 gtatagattg cacaagtcct gaaagcattt gtgcatacat ggttactaca agaatcagtc
      901 gtccagcaaa aaaaataaag gtaatattgg taggagaaaa tcttggtcta taaaatatac
      961 atacatgtaa atagctctat aaataataaa atcatatgaa ataacttatt tctaaaagtg
     1021 gtagaataaa agcttctaca agactttcat tctaccactt ttatattctt ttaatttgtg
     1081 tttctatatt attaattgct ccaattttca tatatattac taactatttt tattttcata
     1141 atttttttgt tcttcatcaa gctgtttata ataatcttta ttatagtcag ctagataatc
     1201 tataccacct cctgtaatca catttaataa tttcattgca gattcttgat taactttaca
     1261 aacataatta tcaccagatt taacaagctc taatgtaata ttaaattctt ttttctttgc
     1321 agaatcaata tctttcatag ttggatttgg catcatttct cctagtttag gaggattctt
     1381 ttttaaaata gaaaccatat cagtattgtt aatttttaaa tctacagtca ctttatcctt
     1441 attattcttt atatctgaaa tttcccaagt catattttta gaatacattt taaatccttc
     1501 tttatcttcg tatataagat tacctaaccc tgattttggg aaatacttat caacagtatc
     1561 tgcatcttgt gtttttattg aattaaacat actatccact tcatttttta tcttattatc
     1621 attagtacaa ccagctaaca ccagtactga tattgcaaac attataatac atagaataaa
     1681 tttcttcatt aaattatatc cccctatact tttttgcaca aatattattc tcttttttat
     1741 cttttgaaaa agttgtagta attgcatttt gtggacaaac ttttatacat tcaccacacc
     1801 taatacagtc tggactattt acatctttat atattggtat atcaagctta cattttgttt
     1861 gacatgctgt acaatttgta catttatctt tatcaatttt aaatctatat attgaaatag
     1921 gattaaaaaa accatagatt gctcctaaag ggcatatata cttgcaaaat ggcctataag
     1981 taatgattga catcaataat atcaatagca aaatacttaa cttccagcta aataaaaatc
     2041 ctattgattc tcttaaattc tcatttccta aaataagtgg tattcctccc aaaagtgttc
     2101 ctgaagggca aatccattta caaaaccaag gatttccacc ccctgctaca tttacaatag
     2161 caactggcaa aataattaca aacattatta agactatata ctttagatat tttagatatt
     2221 tatcaccata aacttttttt atctttactg gaaagggtac tttatgtagt aaatcttgta
     2281 caagtccaaa gggacaaagc caaccacaaa caaatctgcc aaaaaaagca ccaaatgcca
     2341 ttaaaaaacc aacaacataa aatgagaatt tatactctct gcttgataaa actgcttgta
     2401 atgaacctat tggacatgct ccaagtgcac ctggacatga ataacaattc aatccaggta
     2461 cacataattt tttactttct cctccatata tagttccttc taaaaatcca tttacatatc
     2521 catttgttaa tgctgtaaat gcaacttgaa taataagtct tatattatta tttaccttag
     2581 ataataactt cattcctctt ctcctaacca ataccaatac actcaagaca aatcataatt
     2641 gctttagtta atactgtatt tgtttctcca cgatatgtac ctaatgcaat aaatataata
     2701 ctaattgcaa taatagaaat gcctttttta ttatctttaa taaagttact cattggcttt
     2761 taccattttt agtctttcat ctataatttt tttccactct tccttactat ttgcacctac
     2821 aaccttctca cccacaaagt ttcctttctt gtctacaaaa taagttgttg gaaactccat
     2881 aactctttta aaatcagttt ttttaagttc ttcagataca gtcaattgtt ggtaagttgc
     2941 atctgacttt ttcataatgt ttttaactaa atttttctct tcatttgaaa gaccagttct
     3001 catatcagtt ttatcaactt ctacaactaa tccctttata gctacattgg aattattact
     3061 ctcatattct tttgataatg cttcaagctc tggcatttca cccacacatg gtccacacca
     3121 agtagtccag acattaataa gagtaagttc tttatttgaa aatatctctt ttgttacctt
     3181 atttccatca acatcctccg tatctaaatt actaaaccat tcactattac tcttgttttt
     3241 ttctactggc ttagattctt ttttgctaca tccagaaatc acacctaaaa caaaaataca
     3301 tcctaaaact accataaaaa actttttcat atctcacact cctagaattt atatagtata
     3361 taaaatggct gcttgaaata aaaataactt ctcttctata ttaaaacatt atatttaata
     3421 aataagaact aatttaaaat aagttgtata aaagatatat agttaaaatc tatttctt
//
LOCUS       DACTGW010000293         3470 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.296, whole genome shotgun sequence.
ACCESSION   DACTGW010000293 DACTGW010000000
VERSION     DACTGW010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.296"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            12..1097
                     /locus_tag="I9573_003430"
     CDS             12..1097
                     /locus_tag="I9573_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAT4798015.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDGSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWVENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDSSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            1151..2275
                     /gene="wecB"
                     /locus_tag="I9573_003431"
     CDS             1151..2275
                     /gene="wecB"
                     /locus_tag="I9573_003431"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAT4798016.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKALVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDSYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2353..3327
                     /locus_tag="I9573_003432"
     CDS             2353..3327
                     /locus_tag="I9573_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAT4798017.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSVTIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDIIRSYNLQLIDNKVETK"
BASE COUNT         1450 a          337 c          609 g         1074 t
ORIGIN      
        1 aggtatataa tttgaggaaa tatatgaata aatttaaatt aaaaagaagc attttaatct
       61 cattaataat cttaattttt atatttatag tgtactttgt aagtattgag ttaaaagatg
      121 ggagtctagt aaataatcaa tataaaggta agattaaatc ggccaattta tctgtagatt
      181 atgaaataaa tcaagttatg aaggatatag ataaattggg attgaataca gtaaatgtac
      241 ctataattat aaatgtagag tccattaatt ctgatattat gtcaatagat aataatagta
      301 aagaaaaggc aattaagtta ataaaaaaat taaataaaaa aggtatttca acaatattag
      361 aggcatatcc atgggttgaa aatggaaaat tgtatgagac tgactggaac cctataaata
      421 aaaaaagatt tttttatact tggcaaaatg ccatattaaa tgaacttata attgatgtag
      481 caaatccact ggatgtaaat gttttaaata ttggttctaa ttttgtacat ttggaagaat
      541 atcaacaaaa ttggggtgaa ataatagatt ttgtacaatc caaatttgat ggtcttgtaa
      601 cgtacagaac taattggtgg tatacaaaaa aagatgattc aagtagtaag ttgttttatg
      661 aacaaaaatt gaacaataat ttttttgata agttagattt tatatctata gcagcatatt
      721 ttgagttaag taataaacct gttaatacag tggatgaatt aatttctgcc ttacactcat
      781 ctactgtaaa caatagagga caaaatataa aacaagaaat atataacctt tataaagctc
      841 atagaaaacc tatatttttt ggagaattag gtttttcaaa tagagaaagt gcaagttctc
      901 agccgtggaa tcatactcct tcaaaagctg taaatggcga agaacaagca aggtgttttg
      961 aggcatataa gaaagttttt gaaaatgaag actggataaa tggtttttct gtgttttgtg
     1021 taggtaaaat agatgacgaa aaaaattttt acccatcaaa agagagtata aaagttatta
     1081 agtcttggta tgaataattt taaaagtgta gaatttatat agattaaata gatgattagg
     1141 agagatataa atgaatgaca tcaaggttat gactgtattt ggaactagac ctgaagctat
     1201 caaagtagca cctttaataa aggaacttga aaaaagagag aatataaaaa gtattgtttg
     1261 cgttacagct caacatagag aaatgcttga ccaagttata gaaaccttca atataaatgt
     1321 agactatgat ttggatataa tggaaaaagg acaaagcctg aatgatataa catgtaagat
     1381 tttaaataaa cttcccttga ttttaaacaa ggaaaatcca aatataatat tggtacatgg
     1441 agatacaacc acaactctag ctacaagttt gactgcattt tataataaag ctcttgttgg
     1501 tcatatagaa gctgggctta gaacctatga taaatattct ccttttccag aggagttaaa
     1561 tagacaactt acaggtatta ttgctgatat gcactttgct cctactaatt tggctagaaa
     1621 aaacttgatt tctgaaggaa aacctaataa taatatattt gtaactggta atactgctat
     1681 tgatgcttta aagatgacaa taaaagaaaa ctataatcat cctataattg atgagattgg
     1741 taacgatagg atgattttgt taacctctca cagaagagaa aatttgggta aacctatgaa
     1801 aaatatattc agagctataa agcgaatagt agatgatttt gaagatgtac agatagtata
     1861 tcccatacat ttaaatccta aaatacgaac tatagctgat gagatatttg gaaaatttcc
     1921 tgaaaaaatt catattatag agcctttaga tgtagctgat tttcataact ttttaaataa
     1981 atcttatatg ataatgactg atagtggagg aatacaggaa gaagctccat cattaggtaa
     2041 gccagtcctt gtgttgcgtg ataaaacaga aagaacagaa ggaattgaag caaagacact
     2101 taaattagta ggtactaatg aagatagaat atataattca gtgagcgatt tattaataaa
     2161 taaagatagc tatgttcaga tgagtaaagc atctaatcca tatggagatg gaaatgcaag
     2221 caaatatata gtggatataa taattaaaaa atttaattgc aaatatttaa attagataag
     2281 gtctaaaaca ttaactaatt gatataagat ttatgataat attaaggaat acaattttta
     2341 agggggagta atatggctag aaaattaata aaaaatttag gtaaaagtaa aagtgtaaag
     2401 agagtgaaac ttttatttaa gaagattttt attactgtat tcatagtagc aagtatagtt
     2461 gctattttta atataacgaa atattttgag gaattataca aagtaaggga tttaaaaagt
     2521 actaaaattg aatactatat ggatgtggct gatgaagcag gagatggaaa ggtccaatta
     2581 agttggaagg ccttacttgc tatagacatg gtaatacatg atgaagattt aagtaatata
     2641 aaaaagaaag acacattgga tataggggaa aagtttatag tagaagataa aaatgataaa
     2701 ggcgaaaagg tgtataaggt aaaaaagttt aataaggtat taagcgaatt gaaatttgac
     2761 tcttctcaaa aaagtagagc aagaaaatac atgaaagatt tggaatacac ataccttgga
     2821 aataaacaat tagatagtag tgatgaaaaa attaaattta taaagaagtt agaagactca
     2881 gctataagag aatatattga ttatggaata ttgccctctg taacaattgg acaagctata
     2941 ttagaatctg gctggggaaa ttctaaactt acaaaacaga gtaataattt atttggtata
     3001 aaagcagata aagcatggaa aggaaaaagt gtagaaattt caacttcaga gcattataat
     3061 gaaaaaattg tagctagttt taggtcgtat aattcattac aagattctgt caaagaccac
     3121 agtttatttt taattaataa taaaagatat agaaaacatg ggttgtttga ggcaaaagat
     3181 tatattagtc aagctcaagc attagaaaat gcaggatata gtacagcaga agataaaaaa
     3241 ggaaatcgta tatatgcaga gctattaata gatattatta ggagttataa tttgcaattg
     3301 atagacaata aagttgagac aaaataattg ttaactttat atgaggagat aacaagataa
     3361 gattcttttc tacaaaaaga atagaaaaat tgtaaaaaaa actaaaaaaa tgtattgact
     3421 tgttatttaa aaggtgatat tattattact gtactcgaaa gagaacaaaa
//
LOCUS       DACTGW010000294         3454 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.297, whole genome shotgun sequence.
ACCESSION   DACTGW010000294 DACTGW010000000
VERSION     DACTGW010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.297"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            271..>3454
                     /locus_tag="I9573_003433"
     CDS             271..>3454
                     /locus_tag="I9573_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HAT4798018.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDIINQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGIGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADVEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWN
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVINLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRG"
BASE COUNT         1292 a          531 c          615 g         1016 t
ORIGIN      
        1 aatacattga aaatacttga ttattgaaaa atggcataaa ataatacctt ttatgccatt
       61 tactattgtt tgatagtttt taacttatga cataagatat tttttaacat atacaataaa
      121 atataaaaaa attgatattg caaataaaaa aaatacatat tagatgttat agaaaaaaag
      181 cgcacttata ggcttaaaat gtcacataaa agagtttcaa agaataatat attatgaaca
      241 caaattttgt aatcacaaag gagtgataat atggcaacag tatcaggaca aatagttttt
      301 gatagaaata gaagtgctac cattgattct ggggactcag gaatagccaa tataccagtt
      361 gtattacaag atatagcaac tggagttaga cttgtggttc tcacagatgc atcaggaaat
      421 tatgttttta ctaatgttcc aaatggtaac tatagaattg tagaagcttt tggaactaca
      481 ggaggagtac caacaccagg gaattttgca acagcgatag taggtccagt tccaattgca
      541 gaaatgccac ctattagttc tgtgacaaat cctcctattg gttcaacaaa cttagatggt
      601 acaacaccta acacactatt aataacagta gcaggttcag atataataaa tcaaaatata
      661 ttaaatgggc ctgtgattta tacacctata gagaatatat tggattcttg cgtgtctgta
      721 tctaatataa accttattac agatgcagat aacggaacat tcggtttttt tccaccaggt
      781 acaccaagta atacaggacc tgaaatagaa ccttacccaa atgtgacgcc agattttacg
      841 tatgttgtac cagacccaac taagtttaca cctttagatg gagaatatac agtacaaaat
      901 attatgacaa atgcaatgag taatgttata ggagcttggt ggcgtattgc tgaccatact
      961 acaggaaatg aaacaggaag aatgatggtg gtaaatggtt ttaatcctgg agctgtattt
     1021 tttaaatcaa cagtacaagt tacacctaac actaattatt tatttagcac ttggatttta
     1081 aaccttttta aggtaacagg atatccacca ccacagctag gtgtaagaat atttgaccaa
     1141 aataataata tactatatca agcaacatta ggagctcaaa ttccagttaa tacaaacgca
     1201 ccagaatgga aagaagtagg tactgtgatt aattctcaaa ataataatca acttacagta
     1261 gaatttttta gtgaaggaga aagcgccatt ggaaatgact atgctataga tgatgtttct
     1321 ttaagagaaa tagctgttcc aacctttact ccaataaaaa caagtaacac agaaactgct
     1381 aatgtaggtg atataatcac atatacagtt acattagata atacttgtga tagcccttta
     1441 actaatgtat tttttaggga tttaattcca aatggtttag agtttatacc tggaactgta
     1501 acggttgatg gagtatctgt accaggagta gaccctaata caggttttcc acttccagat
     1561 attggaggag gaataggggt tgaagtaact tttgatgttg tggttgaaag tattcctaac
     1621 ccaaatccta ctaataatat agctactata gattactcct atacacctgt agaaggcggt
     1681 ataccaaatg atttttcagt agattctaat ccagttcctg ttgaagtaat ctcagcagat
     1741 gttgaagtta caaaattatc tgaacctact atagtgaatc caggagagga attaatatac
     1801 actataaaag tagtaaataa tggaccattc ccatctgaga atgtggtttt aacagatgat
     1861 gtaccagctt ctattataaa tccagagtat tcattagatg gaggagtaac ttttcaacca
     1921 tggactggaa gtttaaatat agggacatta gaagtaggag agacaagagt tattataatt
     1981 aggggtattg taaatccatc aacagtagga attataacaa atacagctgt agtatcttca
     2041 actacagcag acccaaacct taataataat acatctacaa tagagacaga agtgaattta
     2101 ttagcagata ttttagtaat gaaaacagca gaacctaatt cagctgttcc aggtacttta
     2161 ttgagatata ctattcaagt agaaaactta ggaccagcaa atgctgaaaa tgtaatatta
     2221 aatgatgata ttccagcctc tattataaat ccagaatatt cattggatgg aggagcaagt
     2281 tttcaaccat ggaatggaag tcttaatata ggaactttaa actctggaat ttcattaaca
     2341 gttttaattc aaggaacagt tagtttaaat tcttcagaat atatagtaaa tacagcaaca
     2401 gtttcttcaa ctacacctga cccagattta agcaacaata tatctaccat tattacacca
     2461 gttaacccac aagcaggtat ttctattata aaagtagctg acgaggatgt agctgttcca
     2521 ggagaagaat ttgtatatac aattgaaatt tttaatgaag gaccatctaa tgctacgaat
     2581 gtagttttaa cagatgatat accagatgtt attttaaatc cagaatattc attggatgga
     2641 ggagcaactt tccaaccatg gaatggaagt cttaatatag gtacagttgc accagggcaa
     2701 ttaataagaa ttatcataaa gggattagta agttcaactg ctacaggaga tattactaat
     2761 acagcagaag taagtgcaga tgtaccagaa ccagttacgg attcatcaac agtgacaact
     2821 cctatagttc catcagcaga tatagaagta ataaaaacta gcaatatgga tacagcagtt
     2881 ccaggagaaa cttttagtta tactataaca gttataaatt taggaccatc tgctgcacaa
     2941 agtatcgttt tgacagatga tataccagat gttattttaa atccagagta ttcattggat
     3001 ggaggagtaa ctttccaacc atggaatgga aatcttagta taggcacttt ggatgctgga
     3061 gaaataagaa gtattataat aagaggaaca gtgagccaga cagcagtagg tacaattata
     3121 aatacagcaa caataagttc acctacacct gacccaaatc cagataataa tacttcaaca
     3181 gatgaaacag atataagtcc attagcagat atttctgtga taaaaggcaa cgaaccagtt
     3241 gctataccag ggggaagatt tatttatgga attgaaattg caaatgcagg accatctttt
     3301 gcagagaacg taactttaac agataatata ccagcttcta ttttaaatcc agagtactca
     3361 atagataatg gagtgacttt ccaaccatgg aatggaagcc ttaatatagg tactttagat
     3421 gctggagaga taagaaatat cataataagg ggaa
//
LOCUS       DACTGW010000295         3445 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.298, whole genome shotgun sequence.
ACCESSION   DACTGW010000295 DACTGW010000000
VERSION     DACTGW010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.298"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(225..1439)
                     /locus_tag="I9573_003434"
     CDS             complement(225..1439)
                     /locus_tag="I9573_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAT4798019.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            1854..2570
                     /locus_tag="I9573_003435"
     CDS             1854..2570
                     /locus_tag="I9573_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HAT4798020.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            2705..3331
                     /locus_tag="I9573_003436"
     CDS             2705..3331
                     /locus_tag="I9573_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAT4798021.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
BASE COUNT         1312 a          429 c          530 g         1174 t
ORIGIN      
        1 tttattgtct aactaattat aatctatcca aaatgaaaat gggtgtgaga tattattaca
       61 acaaatcgta ttagtattaa aaaagattaa aaaattaaaa aactaaacag ttaaataatt
      121 aaacgattaa gtgactgtca tataacaatt tataatttta tttaacagcc acttaggtat
      181 ttaagagcga tagaattgag tgtatatcaa attataataa tttcctagta aataattgaa
      241 aaataaggta gtccattttt atatttgata tctggtttac cttgaataag tggatataga
      301 taatttaggg cttcatcagt tatataatta cattcttcat ttatccactc tttaggaaaa
      361 tattttgtct tgttagctat tttagaaata tcagtatgac taacttcata cttataagga
      421 ttattgcatg ttctattaat gatagccata actcctgtat atcctttttt agatagttct
      481 atcccatatg caccaacttc agctgcttgt tttaaatcag tatctgaagc taaatgcata
      541 gcacatcttt gtattacacc aagttctaag gtttttactc ttgttgtaat tgatgatgat
      601 actataagat tctttagata gttgcttact ccacctaatt gggaatgacc aaatttatca
      661 tgagcagaag ttttttgttc gcatataaag ttcccgtatt tatcccttat accttctgat
      721 gcaactatat aagccttatt tttttcttta aatatttttc taacatcttc tataaactta
      781 tcttcataga aatcaatttc tggaaggtat attaaatcaa caagtggagt atcattaagt
      841 ctagctagtg aagaagaagc tgctaaccat ccagtatctc tacccatggt ttctactata
      901 aatataccat tatttttgta tatagaaaaa tctaagtatg tttccattat tagtgtagaa
      961 ataagctttg cagcagaacc aaatccagga cagttgtcag ttcctaataa atcattatct
     1021 attgttttag gaatccctat aaattttatt ttacaagtag tgtttttgca gtattgagaa
     1081 aatttcatca ctgtgtccat agaatcattt ccgcctatat aaaataatgt atctatttca
     1141 tattcatcta gtatatttat aactctatca taatcataag aatttatttt ataatcagcc
     1201 agtttgtatc tacaagaacc aagaccagac gaaggtgttt gctttaaatt taatacatta
     1261 tctttgttta aggagtctga atctattata tttttgttta atataccttc aatcccattg
     1321 ataccaaaat atactttatc aaagtattta gtttctaaat ttttttcata aataccaact
     1381 gcagaagcat tgataacagc tgtagggcct ccagattggg caataataca gtttctcatt
     1441 ttacatctcc ctttcattaa tattgttatg aatggtactt tttatcgagt aaatttatat
     1501 ttatagtctg attatatatt aaatgaacta gctatatcaa ttgagttagt atagacaata
     1561 aatgccataa gaatgtggta aaaaactaac tttttatata caattaatgt ctataattta
     1621 ccacaatata aaggtacttt taaggagtag tcaaagtgtt gtgaaaaaaa atatatcagt
     1681 gtaatattaa tttacaaagc aaaataagtt cacagttaac aaaagcagaa taagttcata
     1741 aaagaaatat atacagcaat aaaagtaatg ctaaataaaa gtaatactgg attaaaaaat
     1801 atcaaaaact aatatttaat actaattaaa gtaaaatatg gaggtataca aatatgaaac
     1861 acataaaaat agcagcagga ttagcacatg taaattacgg tcatatatca gccgtagtta
     1921 aagaagcaat ggatgcaggt gtagactacg tacattcaga tgcagcagat atgcatgact
     1981 taaaaaatat gcaacttatg ggtggtcatc aaataataga agcaataaga ccagtgacag
     2041 ataaaccaat agaatgccat atatacacaa gagattgtga tagattattt atagaaaaaa
     2101 tagcagcagc aggatgtaac ttgttgataa taccagcaga acatttttta ggcgctcctc
     2161 ttgcatacat aataaattac tgtagagaat ttggtatgaa agtaggatta acaattggat
     2221 gttacacacc actatgtttt gttgatgaag ctatatatga tatagataga ttgcaaatag
     2281 tcgttcatgg tgttgatgaa actgatggaa aagataactg gggatggaga aagagtgctg
     2341 ttgatttagt aaaaagagct agaaaaatga tagacgaaaa gaatcctaag tgtgaattag
     2401 caatagatgg tggactaaga gcagacaata tggagccatt aatagaatgt aatccagacg
     2461 ttgtaatatt atcttcagca atattcaagg ataaagatgg tataacagca ggtgttaaga
     2521 attgtagaaa agctatagat gaagcagcta caaagtttgg tttagaataa gataataaac
     2581 tatgataaaa gagatgcttt cttaatgttt taaggaagca ttaattttat gaaaaactac
     2641 aggaagctag actaatttaa ttgatttttt atactgaaac aatataatca taggaggtta
     2701 ttaaatgaaa aaagaaatga taaaagatta tttactgata acaatatcag catttttaat
     2761 ggcattagca gttaattttt tctttgcaga acatacatta gctccaggag ggataacagg
     2821 attatcagtc gtacttagca cattcttatc aatgcctgtt gagaatatat cattaggaat
     2881 atctatgcca cttttagtaa tgggattatt atttttaggg aaaggttttg gaataaaaac
     2941 attatatata actcttatgg gtccattatt cttaaaagta ataccacaaa ctcacataac
     3001 tgacaatcta ttagtagcag gagtgatagg tgggttattg gtaggaactg ctatagggat
     3061 agcaataata aggggctgta gtactggtgg aactgattta gttgcaatgc ttattaataa
     3121 ggtaattaag tttctgaagt tgccagttat attatttatg ctagatggat ttataatagt
     3181 agcatctgga ataataagta agaattttat gatatctata tattcattga tatccttatt
     3241 actcataata aaaacaatag gatttgtaac tacaaagttt ggtaagaata ctgaagaaat
     3301 taataatata aataatgctt gcgctcaata aaaataataa aatttacttt aaatataaaa
     3361 gtttaaaaaa tataaaagtt taaaaaatat aaaagcttaa ataaaaagat taaatataaa
     3421 aaatgaaggt gtctcaaaat gaatt
//
LOCUS       DACTGW010000296         3434 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.299, whole genome shotgun sequence.
ACCESSION   DACTGW010000296 DACTGW010000000
VERSION     DACTGW010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.299"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(31..861)
                     /locus_tag="I9573_003437"
     CDS             complement(31..861)
                     /locus_tag="I9573_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT4798022.1"
                     /translation="MLNKLCMYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGA
                     SRNFNFIEDSVNWIVTVANPVNYPNLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSIN
                     YAQNRNKETNLNINYLFQSYLNMNYNDEFDLATLIYCDYGALSTENRRLLMEKIYDSL
                     KPGGKLILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTL
                     VITEKDENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK
                     "
     gene            complement(1213..2115)
                     /locus_tag="I9573_003438"
     CDS             complement(1213..2115)
                     /locus_tag="I9573_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT4798023.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDGQISFFD"
     gene            complement(2165..2626)
                     /locus_tag="I9573_003439"
     CDS             complement(2165..2626)
                     /locus_tag="I9573_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798024.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(2678..3133)
                     /gene="def"
                     /locus_tag="I9573_003440"
     CDS             complement(2678..3133)
                     /gene="def"
                     /locus_tag="I9573_003440"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAT4798025.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(3105..3368)
                     /locus_tag="I9573_003441"
     CDS             complement(3105..3368)
                     /locus_tag="I9573_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4798026.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
BASE COUNT         1145 a          575 c          293 g         1421 t
ORIGIN      
        1 agtttttatt tattcatttt aaaaaaatta ttatttttgt aaaactaatc ccatagtcat
       61 actatcgtca gaatatgctt cacccttaac attactaaaa aactcaacag acttaaatcc
      121 aatattttca acttctaata agaaactttc ttttgaaaaa tagtgattcc aaacataaaa
      181 tacattttca tctttttcgg ttattacaag agtttgttct aatgtattat aatcattgta
      241 tttacaatta tcttgtagac acatatattt ttcattactc caaaatcctc catcttcatt
      301 tatttcccaa tatttaattt cctcaaaatt attgtattta tttattgtaa atacatctaa
      361 tattaatttt cctccaggtt ttaaactatc ataaattttt tccattaata accttctatt
      421 ttcagttgac aaagctccat aatcacaata aattaaagta gccaaatcaa attcatcatt
      481 atagttcata tttaaataac tttgaaacaa gtaatttatg tttaaattag tctccttatt
      541 tctattttgt gcatagttaa tagaacgttt tgagaaatca attccagtta ccttataacc
      601 tttttgtgca aatttctctg catataaacc tggaccacaa cctaaatcaa gtaaattagg
      661 ataattaact ggatttgcaa ctgtaactat ccaatttaca gaatcctcga taaagttaaa
      721 atttctactt gctccttcaa aattagtgtc taaatgtgct tttaataatt gttttgaaat
      781 atactcatca tcccaaaaat taatttcact ttgtttataa agctctggtc tttctaaata
      841 catacataat ttgtttaaca aaacatacca ctccttaaaa tttattttta aaataaaaaa
      901 accacagagt ttactctgtg gtttaacttc aaaaagatga gtttaaataa gagtaaaata
      961 gaaccaaata tttttaagca caacaataaa actatagctt tactttgttt gcttattaaa
     1021 tttgatttta ttcactcttt tccagcaata cttatatcaa cgattttatg atatcaaata
     1081 tcaccgaaca acatcatcct ttcatatttt tattttatga atcaattata tatttaaatt
     1141 ttatatttgt caataaattt taattttaat aaattttaat catcatagta aaaaaaaatt
     1201 ttttatataa aactaatcaa aaaaagatat ctgtccatct cctgagtttt ttaacttttc
     1261 cttcattatt ttagagtaac tgcttgatag agaatattta tatctaagtt cattaaccat
     1321 tttatacaat tgatttttat actccctatt aagtgaacct gcactatata gtattgataa
     1381 tttatcaaat aattcaggaa actctttttt aacaaagtca aaaaaaacac ctctagttat
     1441 accccttaag tataaagtcc caggtaaaac ataatgtaca ttacattctt ttgcatgtct
     1501 aaatagcgaa tttatattat caaaatcatc tgtaatatat ggtataattg gcataatatg
     1561 tagaccaact gatgcgttag tttttctaaa ttcttttaac attttaaatc gtctcattga
     1621 atccacacca tttggctcaa taagcctttg tgtatcttca tttactgtgg taattgtaga
     1681 tgctacattg atatatgtaa tcctagatag cttatcaatt aaatcgtaat ctcttaaaat
     1741 taaatctgac tttgtagaaa taatagctgg tgtcttgtat tttatcagca ggtttaatat
     1801 ttctggcata attttatagt cagcttctat tggttgataa ctatctgtaa ctcctcctat
     1861 attgattact tctttcttcc atttactgct tttaagttgt ctctcaagtt tttctactat
     1921 atttgttttt acataaatct cttcaaaata attattggag ttaatgtact tatgagagta
     1981 aatagcataa caatatttac atccatgctc acatccacga taaatattta aatcccattt
     2041 atatggcata ctccttttta atttattaca agctatttca caatttattg gaatatattt
     2101 tattttatta tacaaataat cacctcttta tatacaataa tactataatg ttaatctata
     2161 gtaattaaaa catgatattt ccagaccaca attcataatc tttactatat ttacaaagta
     2221 atatattcca ttcatttatt ggtattcctc cacatgttgc tttttcttct aaattatata
     2281 agcattgctt taaatacatt ttattatgcc aatcatcata taaattttct aaatttaaac
     2341 tatgttcatt agatatatca tttgcaaaat ttctattttt ccattttaat atacttgtat
     2401 atttaagttc attaggttta taatttcttt ttaacatttc atcatgtaca atttttgtat
     2461 aaaatttaaa ttcatctata ctataattca atactttatt cactaataga tggtttggag
     2521 tacctttttt atctatagaa ccttttatag caagtagctc tctccattga cttactaatt
     2581 gatttttagg taatacatca attaaatcct tatgccataa tctcataatt taactcctta
     2641 ctataaaata ttttataaat tcaataacca atctatctta caattttaca tgttcaataa
     2701 ttttatctgt aaaaactata ccatttaaat ggtctatctc atgacaaaga cattttgcca
     2761 taaaacctga acctttaatt tctattttct caccgtattc gtttagagct tgcactgtaa
     2821 ctttttttgg tctttttaat ttaccccata cttctggaaa acttaaacat ccttcaacaa
     2881 caatttgttc cccttcttgt ttaataattt caggattaac aagcttaata agtccttctc
     2941 ctaaatctat aacaactaat cgctttaata ctcctacttg gcatgcagct aatcctcctc
     3001 catttggagt attgtacatg gtctctgcca tgtcatttaa tatttctctt atcttattat
     3061 ctacgttttc tacatactta ctcttttttc ttaaaatttc atcatcaaat gttcttatct
     3121 ttcttatagc cataatattc tcccctactt atctgtgctc ttgattttct ttcactttct
     3181 tcaaataaaa atacactttc aattggttca ttaaaaattc ttgaaatatc atatgccaac
     3241 catattgaag gttcattttt accagtttct atagcattta tagcctgtct agatgttcca
     3301 actaatcttc ctagttgttc ttgtgtcaat cctaattttt ctcttaatat ctttatttta
     3361 tttttcatat ttgctccatt catgtaaggt taactttaca tcttaaatgt aacatttttc
     3421 aacattactg tcaa
//
LOCUS       DACTGW010000297         3429 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.300, whole genome shotgun sequence.
ACCESSION   DACTGW010000297 DACTGW010000000
VERSION     DACTGW010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.300"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            145..1878
                     /locus_tag="I9573_003442"
     CDS             145..1878
                     /locus_tag="I9573_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT4798027.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDVFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   2204..2956
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2270..2298
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
BASE COUNT         1362 a          469 c          444 g         1154 t
ORIGIN      
        1 tactgaagca tatattgttt cagttctttt ttgttaaaat taacttattt gacaaagata
       61 tcatagttta acaaagcttt taatttaaat atattgatgt ataatataat taaaatataa
      121 tagcaaaaag aaaggaggaa tatcatgagc atactcctaa aaaaagcacc caaattagca
      181 aaacatatta taacaagttt ttatataaat cgagatatca atgaagtatt aaaatacctt
      241 tgtgaaaatg ttacatggat tggaccagga gaacaggaat ttctcacttc ttttaatgaa
      301 ataaaaaatt atttttatgc aggtcaagat gaaattcctt cttgtgatat aaataatgat
      361 gtatttgaaa ctgtaagtga agatgagaat agatgtatgg tattaggaag atatactgtt
      421 cgaactaaag aaaatgctca aatgatttta gaggtaaatc aacgttgtac atttgagata
      481 attgaggata gaggaaaatt attagtaaag cacatgcaca tatcaaaccc ttatggtgaa
      541 atgcaacttg atgaatactt tcccactaaa attggaactc aaagttatga ttatttgcaa
      601 agattactaa aggaaaaaac agaagttatt gaaatgatta caaataatat caatggtggc
      661 ttaaaaggta gcaatgatga tagtacatac tcatttttct atgtcaatga gggattgccc
      721 aaaatattag gatacacata tgatgagttc atggaaatga gtggtggttc agcagtgggt
      781 gctgtatatc caccagactt acctaaagca ctagaagatt gtgaacgatg ttttgcaaaa
      841 ggacctacat attcatctga atatagaatc cgaaaaaaag atggaacatt aatgtgggta
      901 ttagattctg gaatgaaatc gttaaatagt gatggcatag taaaaattaa tagtattatt
      961 actgacataa cccaactaaa aaacatagaa tctgaactaa aattagaaag agagcgatat
     1021 cgtattgctt tgcaaaatat tacagatatc atgtttgagt atgatattga aaacgatacc
     1081 cttataaaat atcaacgtgt agaaattgac aaaaaaattg aattagaaaa ttttgaaaca
     1141 aaaaattact ccaaactatt agaatctggt aaaattattc accttgatga tattggaaaa
     1201 ctactagaag tacttcatgg aaatcttcgt gaaacaattg agataagaca agtaaattca
     1261 ttaactaaaa atgaatggag atggattcgt gttcaatgtt cagttatata tgatagtgac
     1321 cataacccta tcaaaacaat aggcgtttta aaagacataa ctgaagataa atctaaatta
     1381 gaaagcttaa ttaatcaggc tcaacgtgac ccactaacac aattatataa tcaaagagta
     1441 tcacaaaatc ttattgaaga gtatctatgt agttctgatt ccaaaaataa tgatgctcta
     1501 ttagttattg atattgatga ttttaaaact gtaaatgata cttttgggca tttagaggga
     1561 aatgaagttt tagttgctgt atcaaaaata ctattacata acacacatga taaagacata
     1621 gttgctcgaa ttggtggaga tgaatttaca atatttataa aatcattaac aaaggattta
     1681 ataataaaaa ttactaatga tattttaaat gatgcaagta aaataaaagt aaaggacaat
     1741 cataaaataa cattaagtat tggtattgcc ttcactgatg attcaactaa attatataaa
     1801 gatttatttt ctaaagctga taaagctctt tatctatcaa aagcagatgg caaaaattgt
     1861 tatagtacat acgaatgata aagctattta tacgaccaag aatgaacctt tagatttaat
     1921 ttcatagtct aacaatcaaa gagttgaagc taatcttata gctagtcaac tagttcctag
     1981 aaaaaattta gacaatatat ttattgatac tgctcatttt tctaaagatc aaatttatat
     2041 aaaaaattaa acttttatgt acatacagat actaatgata aacatttagc attagtatct
     2101 tctatcttaa aataaaatga taatttatct atataaaaaa gtgctttgtt gacagagttc
     2161 acttttttat ataggtacag aatttaagta taaatacatg ttttattcta tacattttag
     2221 ttaatgtaaa atttcttgat tgtactctct aacttttcaa cattatacta tttacatacc
     2281 acttagttaa tataaaacca aaagttggag aaatagaagt gccattcaca ttcgaattta
     2341 cataccactt agttaatata aaacacttta ataattctac ctttactagt gctaaagtaa
     2401 tttacatacc acttagttaa tataaaacca ggaacatatg gcaaactata aggaaaacct
     2461 gcaaatttac ataccactta gttaatataa aaccctctgt gactccatat tttttcataa
     2521 tatataattc atttacatac cacttagtta atataaaaca tagaagttgt tgcatataac
     2581 tacattgaca agttattatt tacataccac ttagttaata taaaactttt ctactttttt
     2641 aatcatttaa tcacctgctt aaaaatttac ataccactta gttaatataa aacttttgat
     2701 attctaaaga agtgacaatg atgtgacaca aatttacata ccacttagtt aatataaaac
     2761 tattatgtag tcatatattt caaactctaa tttcgtattt acataccact tagttaatat
     2821 aaaacccatt tgacccatgc atattgactt ccaccttcct tatttacata ccacttagtt
     2881 aatataaaac tttacaatat agacgtacat atacttctga aaaacctctt tccataccac
     2941 ttagttaata taaaacttta ccccaaaata aactgggcat tttcaatact tacacatgtg
     3001 caactctctt aaatttgcag tgaaccatga gtagtgcaaa tgataaagtt tatcatatac
     3061 cctcaacacc ttatatttca agcattaagc cctactttgt aacaaatatc gctcactgca
     3121 aaactattta catttttatt atatcataaa tatattattt ttgaatatct gtaccaattt
     3181 gtggtataat aaaaacaaga agaactacaa tctatttaga cgtagagtga agttcaaata
     3241 ctaatgttta ttctttttga acttgatttt tagttcaaaa ctaaagtcac tcctgctgaa
     3301 ctatacccac tgttttatac agttaaaaca tgtaaaagta gttataaagt actagtgtaa
     3361 ctcattagta ctttttttat tatattcata taattaatat ctatatttta aagtttacta
     3421 tctaaaaaa
//
LOCUS       DACTGW010000298         3407 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.301, whole genome shotgun sequence.
ACCESSION   DACTGW010000298 DACTGW010000000
VERSION     DACTGW010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.301"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            54..1490
                     /locus_tag="I9573_003443"
     CDS             54..1490
                     /locus_tag="I9573_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HAT4798028.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPIVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
     gene            1738..3174
                     /gene="miaB"
                     /locus_tag="I9573_003444"
     CDS             1738..3174
                     /gene="miaB"
                     /locus_tag="I9573_003444"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAT4798029.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT         1414 a          341 c          585 g         1067 t
ORIGIN      
        1 taagaatatg tatttatata ttaaaaattt attatgaggg ggacaatttt attatgaaat
       61 tctataaaag aatattaaca ttgactttaa gtatttcttt tgtatttgca aatattcaat
      121 tagtagatgc aatatcatct gtagaaaaaa tacaaggaaa aaataagtat gaaatagcag
      181 gcaaaatagc agataaaaat gcatataaaa ctgctatatt aattaatact agtaactcta
      241 ttgcagatgg attaagtgca agtggactag caggagcttt aaatgcccct atactattaa
      301 ctgaaaaaaa tactatacca actgagacat ctgcaagatt aaaaaatgta tcaaaagttt
      361 atataattgg aggaacatat tctattagta catctgtcga aaattcatta aaatctaaga
      421 aaatgaaagt tgtaagaatt aagggaaatg acagaataaa aacaagttat aatgttgcaa
      481 aagaaataaa ttcaataaaa aaagtcaata ctgttatgct aaccaatgca tataaaggag
      541 aagcagatgc tataagtatt gcatctgtag cagcaagaga taaagcacca ataattctta
      601 ctaatggaca aagtatacct ttttcaacta gtggtttaaa gtcttatgta ataggaggaa
      661 cagcttcaat gagtaccact ttagtaaaca atactaaatc tactagacta ggagggagta
      721 ctcgttttga aactaataaa gcaataataa ataagtttta taaggatgct agagagtttt
      781 atatagctgg agcttatgaa ttaacaaatg cacttgtagg atcttcttta tcaaaacatg
      841 gtccaatagt tttagttaat gatggtagca ataaaagcat tttaaaaaat gctaagaaga
      901 taacttctat aggatatata gattcaaata tagtacaaca gtgtttaaat ataacaaatg
      961 gaattggaga tattaatact ggggttgtaa aaaatgttaa acctactaca aagactataa
     1021 aagatggaat gtataaggtt ggaaaagata tatcagcagg tgaatactta attacttcaa
     1081 actcaggaag ttatgcttct tattatgaag ttacaagtga tagtacaggt aatgctgata
     1141 gtatactttc aaatgatatt tttagtggga ctagatatat caccttaaag aatggtcaat
     1201 atattaaaat tgaggattca acaatgacat tagcaaagta tgctaaagcc cagaaggcca
     1261 agaatggtaa gtttggaaat ggtatgtata aaattggatt agaaattcct gctggagaat
     1321 atattataat gtcaaattct tctgatgcat attatgaagt tagaaatgat agcttaggta
     1381 atgcagaggg aatagttact aatgatactt tttcaggaag aagatatata acggttgagg
     1441 aaggtcaata tttaatatta aatgattgtt atttaattga aaatgaatag atgtaattgt
     1501 atttaatagt aaatcatata ttttatttaa ggaaagatgt tttaagtttt aatttaaaac
     1561 atctttttat tattataata attatatgat tttatatttt agaaatatag tatatataaa
     1621 tcattagatt tactattaaa acacaactag aaaaataata atatctttaa aagatttttc
     1681 ttatttgata taattaaggt tatgtgtaat ataagaaatt aactaggagg aatagacgtg
     1741 agcaaaagaa aacaagtaac aatacctata gagaaaatac aagaacaaga taaatatata
     1801 gaacaaatac acagacaaaa tgaagaatac ttcaacagaa ctggaaagag aaaactcgtg
     1861 tttactcaaa cctttgggtg tcaaatgaat gaacatgatt cagaaaaatt atgttcgatg
     1921 ttagaagaga tgggatacca aatgtcaatg atggttgaag aaagtgattt aataatatat
     1981 aacacctgtg cagttagaga aaatgcagag ctaaaagtgt atggtaactt aggacaatta
     2041 aaacatctaa aaggaaaaaa tccagatatg aaaatagctg tatgtggatg tatgatgcaa
     2101 caaccacatg ttgtagagga attaagaaaa aaatataaac atgtagacct tatatttgga
     2161 acacataatc tatataaatt tccgcaatta cttacagaat caataaattc tgacaaaatg
     2221 ttagtggatg tatgggatgt ggatggagaa gtaatagaag gtcttagaag taatagaaaa
     2281 tttgaattaa aagcatttgt aaatataatg tatggatgca ataatttctg cacatattgt
     2341 atagtcccat acacaagagg aagagaaaga agtagaactc ctgaagatat aattaacgag
     2401 ataaaagagc ttgttgcaaa tggaacgaaa gaaatcactt tacttggaca aaatgtagat
     2461 tcttatggta agacacttga aaatccagtt tcattctcag aacttttaag aaaagtgaat
     2521 gatatagaag gaattgaaag ggttcgattt atgacatctc atccaaaaga tatatctgat
     2581 gaagtaatat acgcaattag agattgtgat aaggtgtgtg agtttttaca tctaccaata
     2641 caatgtggaa gcagtagcct tttgaaaaaa atgaatagac attatacaaa ggaatattat
     2701 ttagaaataa tagaaaaagc taaaaaggaa gttccaggta ttgctttttc tacagactta
     2761 atgattggtt tcccaggaga aacagaagaa gatttattgg acactttaga tgtggttgaa
     2821 aaagttagat atgattcagc ttttacattt atttattcta aaagacaggg aactcctgct
     2881 gctaaaatgg aaaatcaaat accagaagat ataaagcatg atagatttaa tagggtgtta
     2941 gaagctgtaa ataggatttc tgcagaaata aatgatggat ataaagatag aatagttgaa
     3001 gttctagttg aaggaagaag taaaaataat gaaaataagt ttgctggtag aactagacaa
     3061 aataaactcg taaatttcga aggtggaaat gatgatttaa ttggtaaatt agttatggtc
     3121 aaaattactg aacctagaac tttttcatta aatgggatat tagtaaataa ttaatattgt
     3181 acaaaataat aaaattatgt aaactaatcg aaatagatgt actatagaac aaattatatt
     3241 aaaaattttt attttaagcg aatttgtcat atgttatgct aatgtaagtt tttgctatta
     3301 ataaaaataa atttgatata agatataatt tcattgtaca tctatttttg ttttattata
     3361 ttttataaaa gcttacttag ttgtcattag taattccatg ttatcat
//
LOCUS       DACTGW010000299         3407 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.302, whole genome shotgun sequence.
ACCESSION   DACTGW010000299 DACTGW010000000
VERSION     DACTGW010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.302"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1385
                     /gene="tilS"
                     /locus_tag="I9573_003445"
     CDS             <1..1385
                     /gene="tilS"
                     /locus_tag="I9573_003445"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAT4798030.1"
                     /translation="FDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKKDF
                     NIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEEGA
                     RKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNCII
                     RPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIESI
                     VRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNSIK
                     HILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEEIE
                     FYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSRRQ
                     GDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDENTK
                     EVLKINFNKL"
     gene            1419..3389
                     /locus_tag="I9573_003446"
     CDS             1419..3389
                     /locus_tag="I9573_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HAT4798031.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
BASE COUNT         1371 a          355 c          705 g          976 t
ORIGIN      
        1 tatttgataa ggtactaagt actataaata aacataattt aatacaaaaa ggcgataaaa
       61 tagttttagg tctttctgga ggacctgatt cagtttgttt attgcatgta ctaaatagat
      121 taaaaaaaga ttttaatata gaaatatatg cagcacattt aaaccatcaa ataagaggga
      181 tagaagctca aaaagatgca ttatatgtat ctaaactttg tgaggatatg ggaattatat
      241 tctttgtaaa gtctattaat gtaccaaaat attgtgaaaa tgagggatta tcattagaag
      301 aaggagcaag gaagctaaga tatgaaatgt tttatgaaat taaggataag attaaagcca
      361 ataaaatagc aataggtcat aatctcaatg accaagccga aactgttatg atgcgtatca
      421 tgagaggaac aggcttaaaa ggcttaaagg gaattgatta tattagagat aattgcataa
      481 ttagacccat attagacgtg gaaagaaatg agatagagga gtattgtgaa gcttataatt
      541 taaatccgag gatagataaa acaaatttag aaaatatata cactagaaat aagataaggt
      601 tagacctttt accatatatg aaggataatt ttaattcaaa tgtaatagaa tctatagtga
      661 ggatgagcaa tagtttaaaa agtgataacg attatattga gaaagaggca gaagctaaat
      721 ttagagaagt ttcaaatata aaagaaaaag gctttgtaga gataaattta gacgattttg
      781 tttgcttaca tgatgctatc aaagttagag ttcttagaaa ttccataaaa catatactag
      841 gagatactaa ttttgttgac caaagacata tagaagatat aatgtcttta gaagataatt
      901 caaaagtaaa taaaatgtta actcttccaa gaaatatatt tgtttataga aaaaaagaca
      961 gtataatatt aacgaatgag gaaattgtta atgaggaaat tgaattttat tacaatgtac
     1021 ctagtaatgg gtttattaaa ataaaagaat taaaacaaat tattgagacc caagtaatga
     1081 gcatagatag gtataagagt atgaaattag acaattcatc taaagggttt gattttaata
     1141 aggtaaaagg gggtatagta ataagaagca gaaggcaagg tgataagata aagcttgcta
     1201 tgggaagtaa aaaagtgaaa gatttattca tagatttaaa aatcccaaga gaagaaagat
     1261 gtaaaattcc tataatcaca gatagtgaag gaataatatg tgttggagat tataaaatca
     1321 gtgaaaatta taaaattgat gaaaacacaa aagaagtatt aaaaattaat tttaacaaat
     1381 tatagggcaa agcaatgtat gaaaggaggg cttctatatt gaataagttg ctaaaagggg
     1441 caggttttta tctacttgtt tttataatta tagtaggtat agttcaattc tcaggtaagc
     1501 ctacagaaaa aataaaagat ttaaaattct ctgaagtata tagagaatta acagatgaga
     1561 atatatcaag attatacttt gtaaatcaaa cttctgtaga aggtactata aaagatacaa
     1621 atacaaagtt taagtcatat gttccaacag aagtgatggg aaataaactt gcagatgagg
     1681 tgttagacca agctaaggct gggaaactaa cttttggtgg tgaagctaag ccatctacac
     1741 cttggtttgt cgaaatgttg cctacattac tgttaatatt cttcatggtg attatttggt
     1801 tcgtgtttat gaatcagtct caaggtggag gcggaaaggt tatgagtttt ggcaaatcaa
     1861 aagctaaggt tcataaagat gatgaaaaaa ctagagttac atttaaagat gtagctggac
     1921 tagatgagga aaaagaagat ttgcaggagg tagtagattt cttaaagaat cctaagaaat
     1981 atattgaatt gggtgcaaga ataccaaaag gaatgcttat ggtaggacct cctggaactg
     2041 gtaaaacata tctatcaaga gcagttgcag gagaagcagg agttcccttt ttcagtataa
     2101 gtggttctga tttcgttgag atgtttgttg gggttggtgc ttcaagagta agagatttat
     2161 ttgaacaggc taagaaaagt gctccagcta tcatattcat agatgaaatt gatgcagttg
     2221 gtagaaaaag aggtgcaggt cttggtggag gtcatgatga aagagagcaa actcttaatc
     2281 aacttctagt tgaaatggat ggatttgggg taaatcaagg tataataatc atggcagcta
     2341 caaatagacc agatatactt gacccagctt tacttagacc aggaagattt gacagacaag
     2401 ttgttgttgg tacaccagat gtaaaaggta gagaggctat atttaaagtt cactcaagaa
     2461 ataaaccatt aagtgatgat gtaaaaatgg atgtcttggc tagaagaaca ccaggattta
     2521 ctcctgctga tatagagaac ttaatgaatg aggctgcaat attaacagct agaaaaagag
     2581 aaaagaaaat taagatggaa actatagaag aagctataac aaaggttata gcaggtgtgg
     2641 ctaaaaaatc gaaagtaata agtgagaaag aaagaagact tacagcatac catgaaggag
     2701 ggcatgcagt ttgtgcccat gtacttgaag aagtaagtcc tgttcatcaa gttacaatag
     2761 ttccaagagg aagagctgga gggtttacaa tgcaacttcc tgttgaagat aagttctatg
     2821 ctacaaaaaa tgaaatgaaa gaaaatattg tagttttact tggtggtaga gtagcagaag
     2881 agctagtact taaggatgta tctacaggtg cttcaaatga cttagaaaga gttacagcta
     2941 ctgcaagaag tatggtaaca aagtatggaa tgagttctaa acttggtcca atgtcatttg
     3001 acagtgatga tgaagtattc ctaggtaata gtttttcaag taagagaaat tactctgaag
     3061 aagttgcttt tgagatagac caagaaacaa agcgtatagt tgatggtgca tatgataaga
     3121 caagaagtat attacaagaa aatatggata gattagagta tgtagcacaa gctttactta
     3181 tatatgaaac tttagatgca gaacaatttg taaaagcatt taataaagag ttacctctga
     3241 atgaaattga aaatgcagtt acagaggaaa attcttctaa agaagttgaa gaacaattaa
     3301 ctataaaatt agaaaaagat gaagaagaaa gaaacaatgt tattgatata aataaaaatt
     3361 tagaagataa atctgataaa gataaatagt tttaagacct tggtcat
//
LOCUS       DACTGW010000300         3406 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.303, whole genome shotgun sequence.
ACCESSION   DACTGW010000300 DACTGW010000000
VERSION     DACTGW010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.303"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(27..872)
                     /locus_tag="I9573_003447"
     CDS             complement(27..872)
                     /locus_tag="I9573_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT4798032.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            complement(903..3137)
                     /locus_tag="I9573_003448"
     CDS             complement(903..3137)
                     /locus_tag="I9573_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAT4798033.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESSRELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLILFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
BASE COUNT         1185 a          466 c          376 g         1379 t
ORIGIN      
        1 ttttaattta ttttaaaata acaatgttaa taatcatttt tatcaaattt tttcttgttt
       61 ttctccttgt ttttatacat ttttttatcc aattcaacta ttgtcaattt taaatttctt
      121 tttactgtat caacaccata actaaatgaa agatacttat atttagagaa tacttttaaa
      181 aaattttctc ttaaggcttc tagttgtgaa taaacataat ttttgtcaac atcactagag
      241 aatataacaa taaattcatc tcctcctaat cttataatag aagtagaatt tattaattct
      301 ttatttaaaa gtgaaacaaa atcacataaa atcttgtctc ctacattatg accttcatag
      361 tcatttatca ttttgaaatt atctaagtca attagtacaa tataatctcc tataaatatg
      421 tcaaatctat catttaaata ttttctatta tatgctccag ttaatgggtc agtaattgag
      481 tagtgaagat ttctcttatt ttcttcatag agttttgtta tatcattggc catagtaatt
      541 atgtaggttt tttcatcaac atctactttg tattctaaaa cattccaata gcttgtagta
      601 tcttttttaa actctaaaat ccaatttgaa attttatcat aaggataatt tttttcccaa
      661 tctaattgaa tattgtgaga atccgtaaaa aatttagcaa tattatcagg gagtttctta
      721 gctatttcta tattagtcat tccaagtgga ttatcttgca tataactttt aattaaattt
      781 tttaaattat catttataaa tataattctg tagttagaat ctcttataat tagaaatcct
      841 ggaaaagttc ttaaaaaaat ttctttaaac atattaattt ctccaaaata aaatactttg
      901 tactagattt ttgttaaatt ttttatttta tttacatcta catacatacc ttgggcaatc
      961 gaccttccat caattgcaga aaggttacat tgtgtataca taactgtgac aatttgacca
     1021 tctcgtttta tgaatctata taaaaatggt tcaattttat ttgtgtcaat catatccaaa
     1081 ctttttttat aaacaaagtc taaatcatct ggatgaacaa ttgctcttaa atttccgccc
     1141 atctcaccaa aaaactgttc ttttgtataa cctataatgt caaacatttc ttcattcata
     1201 taagtaataa tcatatcacc aaataaatcg tagcgaatta tacctacagg caacattgaa
     1261 taaaattgtg ataaaatatt actttcatca agatgtgtat ttgtcatttt aggtattgaa
     1321 tatgattcaa attcatttat atagccagca gttttaatta aatccaaatg taattttgct
     1381 aagagtaatt tattttctga aattatacaa tttgcactaa agtagaaatt gctatatttt
     1441 tgatgttcag aagcttcatc tgataaaacg gcttctccac taattaataa tgaatctgat
     1501 tcagtattat gtggggttat acttttgtaa agaatagaaa attttttacc tttaatatgt
     1561 ttttcaaaat gttctattac tttaggaaaa gatgtaattt cagttttgtt gtaaaaatca
     1621 aaccattgaa tgtcttcagt gacattagct cttagttttt tcatatcttg cataataaaa
     1681 aatccatcaa atatcttctc tattaaagat tttcctatat cagaatattt tataaacatt
     1741 ttctctgaat tattctcttt agatagtaat ttttcaaatt ctttgcactc catgggtttt
     1801 gcataataat aaccttgggc gatatcacaa tttatacttt taataaattc tacatgttgt
     1861 tctgtttcaa caccttcaca tactgtaagc atcccaagag ctcttgcaat gtttattata
     1921 gagtacaaaa tggttgtatt tttaaaatca tttaaattat gagataaaaa tatcctatca
     1981 attttgatta tatcaaaagg tagattttgc aacataccaa aagatgaata tccagaacca
     2041 aaatcgtcca ttgatacaga aaaaccataa gaacgaagtt cagctagttg ttgaataatt
     2101 gtatttgtat tttctattaa tatattttct gttatttcaa tgctaaaatt tgatgtagag
     2161 acgctgtact tttcagtaat accttttagc atacttggaa aattagggtt aaaaaaatga
     2221 agacgagaaa agttacaaca aattggaaca aaaacatcat tgttatcaat ccatcttctt
     2281 atatttttac agttctcttc aaataaatgc aaatctaatt ccgttatcag accaaactgt
     2341 tcaaataaag gaataaattt atctggagta agaagtcctt taatagggtg tctccatctt
     2401 gcaagtgcct cacatccaac tatagtatta ttttttatat tataaagtgg ttggtaaaat
     2461 acttcaattt gtttagtttg tagaacatgt gatatttctg ataagaactc cttatcattt
     2521 tctaatttat tgtaaaaact attatcttta aaatcaaata gaatgtatct atttttgcct
     2581 tcacttttag ccttttttaa agcactatat acttggttga aaagtatcaa agtagtcatg
     2641 tttggttcac cattccagat agtgctgccg atactaaatg taacattgga gtatatttct
     2701 ttttcattag gaagtcttat ttttaattct cgggagctct ctataatagt tgcacccata
     2761 ttatttaact ctttatttga tacatttgga agtacaatta aaaaaccttc tccttttaaa
     2821 taagcgacta ttccctttga ttctggaata aagtgcaata ggttttgagt tagagtaatt
     2881 aataaatttt ctccgactag atatccatat atatcattaa ataatttaaa gttatcgata
     2941 tttaataaca tgattcctat atgtatattt ttcgaaatac agtcatcaat atattggtac
     3001 ataaattctc tgtttggtat tttagaaaaa aaatctattt catttaaggt ttttaattta
     3061 aaatttctac tatctaattc cttagataat acttctacct ttttatttaa catttttata
     3121 taattatgat tttgcatggt attcctccat aagatacttt aaattttgat gacaactgta
     3181 tttatgctag aagtatttaa atgttaattt gtaaataatt taaatgctaa attttaaata
     3241 agtatttaaa atgaatatac attaagttat ttgctccaat gctacaaata caaatatttt
     3301 ttagtttagt aataatatta gataaaatta gttaatgctt aaattatagt atattgaaag
     3361 atttcctttt tgaatgcaat tatatcatat tttttcataa atagat
//
LOCUS       DACTGW010000301         3401 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.304, whole genome shotgun sequence.
ACCESSION   DACTGW010000301 DACTGW010000000
VERSION     DACTGW010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.304"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(130..627)
                     /locus_tag="I9573_003449"
     CDS             complement(130..627)
                     /locus_tag="I9573_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAT4798034.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            complement(656..2035)
                     /gene="scfB"
                     /locus_tag="I9573_003450"
     CDS             complement(656..2035)
                     /gene="scfB"
                     /locus_tag="I9573_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HAT4798035.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(2340..2486)
                     /locus_tag="I9573_003451"
     CDS             complement(2340..2486)
                     /locus_tag="I9573_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HAT4798036.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            complement(2516..3094)
                     /locus_tag="I9573_003452"
     CDS             complement(2516..3094)
                     /locus_tag="I9573_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HAT4798037.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKFVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
BASE COUNT         1187 a          616 c          306 g         1292 t
ORIGIN      
        1 ttttgacatt ttatatctgg tttaatcaaa tactttttga aaactaaaat atcaacaaaa
       61 tctaataaaa taaattagtg gttgcctcaa aacagattta aatatgtttt gaaacaacca
      121 ctctttaaac taatattttt gattttgtgc tgcctctaaa taccattcat aagattcatc
      181 aatatatttt ttatcttctt ctgtaagctc tctaacaacc ttaccaggag aacccattat
      241 caaaacacct ggtggaaact tcttattaga tgtaattagt gtacctgctc ctatcagagt
      301 atattctcct atttcagcat tatccaatat tattgaaccc ataccaatta acacattatc
      361 actaatttta caaccatgaa ctatagacct atgcccaact gttacattgt taccaattat
      421 agtttctgta tctccatgca ctatagaaca atcttgtata ttagtgtttt ctccaatagt
      481 tattggtcct tcatcacccc taactactgc attgtaccat atacttgaat ctttgcctat
      541 tttaacattt ccaattacat ctgctgattt tgccacaaat acactttcgt caattaaagg
      601 tttatcttct aaataatctc taatcatata taaacaactc ctttaaaata taaatttagt
      661 tggttgctcc ttctaacatt aacttagctt gtatcattat agagcactct gtacgttttc
      721 tttgcatttc acatcctaat tcatatggtt ttgatatgtc attattaaag tttatcgcat
      781 ttgcatgaca tccacctgaa cagtaaaact tattccaaca ttgtttacac tcttctttag
      841 tatatacatg agcttctcta aacatatttt ttaaattttc tggaagtact atttcttcat
      901 caaatatatt tgccatttta aattcttcat ttccaacaaa ttgatgacat ggatagatat
      961 ctccatttgg agttacagaa agatactcat ttccagctcc acatccagtt attctcttta
     1021 ttacacatgg accttgattt aggtctatca taaaatggaa aaatttaaat ccatctcctt
     1081 gtaattgtct atctgcatac tctacagcaa atttttcgta ctcttcaaat atcttaggta
     1141 aatcttcttc tctaagtgca tatggattac tctcatctcc aacgactggc tctacagagg
     1201 ttaatttaaa tcctaaatct gcaaaatgca taacatcttt tgaaaaatct aaattatctc
     1261 ttgtaaaagt acctcttata taataatact tatcttttgc tcttttttcc acaagtttct
     1321 taaatcgtgg taatgtaata tcataacttc ctttatcatt taaagttggt ctcatattat
     1381 cattaacttc ttttcttcca tctaaactta aaactacatt atgcatattt tcatttatat
     1441 aatctataat ctcatcattt aacagtacac catttgtagt tattgtaaat cttatatttt
     1501 tattataatc tttttctaca cttcttccat agtcaaccag ttgttttaca acttcaaagt
     1561 tcatcaaagg ctctccacca aagaaatcta tctctaggtt ctttctactt cctgaattag
     1621 caatcaaata gtcaattgct gccttaccaa cttcaaaact cataagttct ttttctcctc
     1681 caaaatcacc ttgagctgca aaacagtatt tacattttaa gttacaatca tgagctacat
     1741 ttaaacataa agcttttact acaggctctc tatgtacaaa gcttggatga tattgatatg
     1801 tatcttctgt gtataaaaga ccttcttttt ccaaatttaa tatttcttca tatgcttcat
     1861 ttattttttc ttcttgatat tttgatttta atatctctaa tatttcttct ttactttttt
     1921 ctttataaaa atctaacaaa tcgtatgcaa catcatctaa cacatgaaca gctccaccat
     1981 tcacatctaa aacgatatta tatccattca tagaaaattt atgtatcata ctcataccct
     2041 acctcctaaa ttactatcta tactaaagtt ttttattatt ttaattaaac tttacccttc
     2101 attatatcat aagtttacat aaaatacata caatacaaaa atgacttact ctccgtaagt
     2161 catttcatta aatacactat atccccctaa attggcgcac taatatatta ccaaataggt
     2221 attaggctcc tataaaataa ataaaataaa ttttgtatct gattttatat tgttttaatc
     2281 ttattaaaat ataataattt cttttttcct gataattttg tataaataag tgcttgtatt
     2341 taatttgtct tctttagatt gctgatttct ttgggtactt taggctgatg aataatccaa
     2401 ggacaggtac tattagctga caaaatagca gtgctaagtg ccaatgagct agcaaacttc
     2461 ataaatctta caataaactt tttcataact taactctccc tcatacatat tttacttata
     2521 ctttagcttt taacttacct aatactaaca tcacaaaaat ccaaaacatg gcaaatgcgc
     2581 tatatgtgaa ataatctaca aatatactca aagaaagact taaaattgtt atcaataaca
     2641 ctactgtaga tataaatata gcagttttct tataaacaaa cttttcccta tcacttatag
     2701 ggttactcct atgttctatt ggacacagaa cacaaattcc aacccaacta acacttgcaa
     2761 taatcataat aatattttta aataagtcta tatttatatt ttctataatt aagattgtag
     2821 ataaaattat aaaaataaag gttaataagc atctaaaata attgtctgca tgataccctc
     2881 ctgaaaattg tcttattgga caataacaca ttaaaaaaaa taatacatat gtaaatttac
     2941 caaataaaaa acctataaac agcatggtgg ttatattaac tatgaaagct attaatattt
     3001 caaatccata agaatagact ttagattcat tattatctat catattatta caaattaaaa
     3061 cagatgtcat tttttcagca tatcttttaa acatttttac ctccaaaaaa attttacata
     3121 tatagagtac attctttttt gagaaaattc aatatacttt acaaaactag ttgttaaaaa
     3181 tgcaaaactg gtcaaaaaaa gacctatata attttcttat tattatttat ataacaaaac
     3241 aattataata aaacaatttt atcaagcttt atatgcataa aaagcctatg tcaaaataga
     3301 aataaacctt ttccccattg ttaaaaggct aaaaatatat tttatttcat ataaaaagag
     3361 tacctcacaa actaaaaagt tcattttgag atacccattt t
//
LOCUS       DACTGW010000302         3369 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.305, whole genome shotgun sequence.
ACCESSION   DACTGW010000302 DACTGW010000000
VERSION     DACTGW010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.305"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(145..666)
                     /locus_tag="I9573_003453"
     CDS             complement(145..666)
                     /locus_tag="I9573_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAT4798038.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENIIIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
     gene            794..2158
                     /locus_tag="I9573_003454"
     CDS             794..2158
                     /locus_tag="I9573_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAT4798039.1"
                     /translation="MKESNKKIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDKLHIAIKTSILWTTIFCVVLGL
                     ILVLFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            2347..3024
                     /locus_tag="I9573_003455"
     CDS             2347..3024
                     /locus_tag="I9573_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAT4798040.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSDYKKGNPVH
                     CGDSDCIGILIIKSGTIRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQINSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
BASE COUNT         1072 a          463 c          513 g         1321 t
ORIGIN      
        1 aaaagtttaa cattctccct caattaagtg agctttattt taaatatact tttcagttta
       61 tatcatttgt ttcatttttt ctaatatctc attattaaat atgctggttt ttgtttaatg
      121 attattaagt tagcttctct aattctaatt ccccatatta tcttttaaag aatttatcca
      181 ggtttccaat gcttgtgcta gcaccaactg ttgctccaac actgcttttg ctgtctctgg
      241 tacttctgtc tttataatta tattttcctc aatagttttc tttaactctt gaacattctc
      301 ttcaatatta ttgagacata atttctgttt ttctggtgta aggctattta aatttactat
      361 aacagcatta aaatctaaaa gaatatttat tggtttgcta gatgtatcaa acattaaatc
      421 taaaaaatgt gtctcaccta attcagttaa tgaataaact gctttttctg gcatttttcc
      481 attcttcaca gttttacttt caatatatcc cttttgttct aattgaatta cttttttata
      541 aattgatggt gtacttattt taacccaacg tgatatattc ctgtattcca ctgccttttg
      601 aatatcataa gcactcttgg gttgttgctt tactattccc aatacaatta aatctattgt
      661 agccataaaa atcatctcct ttagttttct tactattgac aaatactata aattatagta
      721 ctattttcca tacttactat aatttatagt attataattt agacttaatg tcaatagaag
      781 aggagtgtta agtatgaaag aaagtaacaa aaaaattgaa ttattagggt ctgccccaat
      841 ctcaaaagca ttgttatcta tgggattacc taccatgata ggtatgatga taactgcatt
      901 atacaatttg gttgatgcat actttgtggg tggacttgga acaagtcaaa tgggagctat
      961 atccgttgca tttccattag gtcagcttgt tgtgggatta ggattattgt ttggaaatgg
     1021 agctgcttca tatattgcca gacttttagg ccatggtgat aatgaaaaag caggaaaagt
     1081 ggtaagtaca tctatttaca gtagtattat agtgggtaca ttagttatta ttgtgattat
     1141 gatttttctc aatccaattt taaaattgtt aggagctaca aaaagtatta tgccttatgc
     1201 aatatcttat gctagcattt atgtcgtgtc atctattttt aatatattca atgtcactat
     1261 gactaatatt gtcacaagtg aaggagctgc taaaacaact atgtgtgcta tgttattagg
     1321 tgctttgctt aatatgattt tagacccgat attcatttat acttttggtc tgggggtaag
     1381 gggtgcttct attgcaacag ctatctcaca gattgcttca acacttgttt atgtaggata
     1441 tattttaagc aagaaaagta tatttagttt tagaattaac gaatgctgtt tttcaaaaga
     1501 aattttttca gaaatcctaa aaattggcat acctacatta atatttcaat tattgactag
     1561 tcttgccatt acattaacta atatggaggc tacaaagtat ggtgattctg tgatagctgg
     1621 aatgggtgct gtaacacgaa taacatcttt aggtagtctt gttgtatttg gttttataaa
     1681 aggtttccaa ccaattgctg gttataattt tggttcgaag aactacgata aattacatat
     1741 agcaataaaa acttccatat tatggactac aatattttgt gtagtgcttg gtcttatatt
     1801 ggtgcttttc ccaaaatcta taatttcaca atttacaagt ggtgacattc tattaataga
     1861 aaatggtagt aaagctctta gggcaaatgg gttttcattt atgctatttg gattctatac
     1921 agtgtattct acattattcc tagcactagg aaaagcaaag gaaggtggat ttttaggtat
     1981 gtgtagacaa ggcgtctgtt ttatccctat aattttgatt attcctgtat tctatgggtt
     2041 aaatggtatc ttatatgcac aaccaatagc tgatttgatg gctgctataa ttgctggttt
     2101 tatgtctata aggttacata aggaacttaa tcttttaaaa ataaacggta tgcattagtt
     2161 atacaaacct actatcatca aaacatatta ctttctcatt aatagcaaga atatatatta
     2221 tcatattttt tctacataaa tcatactatt actatctgac taaataatcc tcttaacaaa
     2281 ctacttgtca atttatctat tcacttgtat catgggatta taacaattta agaaaggaga
     2341 tattgtatga tttcacaaaa tgaaattgat atacttacac atagcttgcc tttttgggat
     2401 aaacttactg acttacaaaa agaattatta atttcttcag caaatacctc ggattacaaa
     2461 aaagggaatc cagtacattg tggtgatagt gactgtattg gtattttgat tataaagtct
     2521 ggaacaatac gtacatacat actttcagat gaaggtcgag aggttacact gttccgactt
     2581 gatgatggag atgtatgtat tttatcagct tcctgtattc taaaaacaat aacttttgat
     2641 gtctatgtag atgctgaaac ggactgcgat attatacaga ttaattcctc tgtttttgca
     2701 aaattatcta cagaaaatat tcatgcagag ttattttcat ataaattagc taccgaacgt
     2761 ttttctgatg ttatgtgggc tatgcaacaa attcttttta tgagttttga taagcgtttg
     2821 gcatcttttt taattgatga gatagctaaa aatggcagta gcactataaa tatgactcat
     2881 gagcaaattg ctaagtatat gggtagtgcc agagaagttg tatcgagaat gttgaaatat
     2941 tttgctagag aaggtattgt gtctttatct cgtggtggta taaaagtatt ggataaggat
     3001 aaacttcgtt ctttgacact gtaaaagtgt gtctcaaaat gaacttttta gttcatgagg
     3061 cactcttttt tgtgtgaaat caaatatatt ttcataattt caataataaa aaagatactt
     3121 atatctattt ttatatagta tcttatttga tatttacatt tattttttat gtatattttt
     3181 atattttaat aatttagttt aactattttt ctatgctatt tatatagaat atttctaata
     3241 actcataaat tctaatcttt taatatctat ccctcaatta atggatatga atttatttaa
     3301 tttaaatact tgatattttt catatttata taaaaatatt acattttaaa tacaagttaa
     3361 taaaaatgg
//
LOCUS       DACTGW010000303         3364 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.306, whole genome shotgun sequence.
ACCESSION   DACTGW010000303 DACTGW010000000
VERSION     DACTGW010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.306"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..515)
                     /locus_tag="I9573_003456"
     CDS             complement(<1..515)
                     /locus_tag="I9573_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAT4798041.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFV"
     gene            complement(726..1385)
                     /gene="cprR"
                     /locus_tag="I9573_003457"
     CDS             complement(726..1385)
                     /gene="cprR"
                     /locus_tag="I9573_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HAT4798042.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            1696..2325
                     /locus_tag="I9573_003458"
     CDS             1696..2325
                     /locus_tag="I9573_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HAT4798043.1"
                     /translation="MDENITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            2393..3226
                     /locus_tag="I9573_003459"
     CDS             2393..3226
                     /locus_tag="I9573_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HAT4798044.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPLGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
BASE COUNT         1249 a          503 c          373 g         1239 t
ORIGIN      
        1 acaaatgaat taatggctat ccctagaaac actagaggca taatcatttt catttgtttt
       61 tctaacatag caaaattcat taatacaagt attgtacaat aaaaagtaac cactatttgg
      121 aaaataatag acttattggt aacaactgca cttgccttac taagtggcat tttacatttt
      181 ttaagtacat atatctgtac tggttggctt cctgaagcaa aaggagttat taaattataa
      241 taaaatccca taatggctag cttaaaacca ataaatctaa tattaacttt gtgagtgctt
      301 tctataatta tcttcattac tactccttca agcattatgt gacacattat tgccaaagca
      361 cctatgaata aaaacttctt atctaccata accataacat ttgatagcat tttaatatcc
      421 aatgttttaa acactaggta aattgttata cctattaaca aaaataaaaa tgtatactgt
      481 agtaaactct ttctaatatt ctttagtcta tccattagaa ataaccatcc tttgctcaag
      541 atttatacta aatactatat tattagtata cactatgaat cttaatattt tataacaaaa
      601 taccttacaa ttttgtcatt tacaaaaaat attaatctat aaattaaatt ttaatatata
      661 gattaatatt ttacaaacat tttgtagata tatataatat tttcactaag caacacttat
      721 acttattaca cccatttata ccctatcccc catacagttt ctattgtata aatatcatat
      781 tgttttaatt tgcttcgtat attttttata tgctctgtga tagtacttat atcactttct
      841 ccatcaaaac catataccga ctcatatatt ctttcttttg aaaatacttg tcccttattt
      901 tttgctagaa attcacatat tagatattca cttttagtaa aattaatttt tttaccatca
      961 attgaaactt ctttgcctaa aaagttaaat tgaacatttg agatagttaa taaattcttt
     1021 ttttctctac tttctcgcct taaatgagca gttatcctag cccttaattc tcctacacca
     1081 aaaggtttgg ttatatagtc atctgcacct atgccaagac cgtacataat atcactttcc
     1141 atattttttg ctgtcaaaaa cagtattgga caatcaacct tatttcttat agttttacat
     1201 acttcaaaac catccatctt aggcatcata acatctaata ttattagatt aaaataatta
     1261 aaatccatgt tcatagcctt ttctacatca ctaattacct ttacttcatg tccatctctt
     1321 ttcaacacat tttcaatcaa ctttaacata tctaactcat catctactac taatatctta
     1381 gacataataa ctcctccatt tatccgttta atagtattct aatttatatc tttaataata
     1441 tctttaaaat tcacattaac cataatactc tcatattata gcttacaata attaattact
     1501 attttacaaa taaaatattt tagatacaaa aactttatat ttcaaaaact ttatattcat
     1561 tttacacgca tttacttaaa tataaaaatt tatttaacaa tttattattg taatattttg
     1621 aatatataat aaaattaata ataagaaaac tttttataaa tacactttta attgtttaaa
     1681 ttggaggaaa aaattatgga tgaaaatata acaaaaaata attataatgt tccatttgac
     1741 tatgttcgaa aaaaactcaa agaattagac cctcatacaa ttagtaattt aagtaatata
     1801 cctttcaata atgatttaaa tagattcaca ctcaattttt ttgatagaca atatattata
     1861 gactatccaa gtggagaagt ttggaatagt aatggtagcc tttgcaataa ctttgagctt
     1921 aaaatcttga tagttagata tttaatcaat gctaaaggta tccctagaac taataaatat
     1981 gtaactttta aagaaatacc aggaggcaat gtttattatc caaatttttt aaacaggact
     2041 ctatataggc tttcagaagt ttttacatct caaatagaca aatataagtt agttatggag
     2101 aaaattggag ctgaaaaagt aaacatggga gatttagctt ataggtttac atatatgggt
     2161 aatacatcta tgatatttat actttggttt ggagatgatg aatttcctcc aaattcaaat
     2221 atattatttg attctaatat agtttattac ttcaatgcag aagatttagc agttgttcct
     2281 gatacagcta taaatgcaat tttaaataaa ataaatcaat tataaatata aataatatta
     2341 taataaagtt tttgatagat ttaatagaat ataatatgaa atggggtaaa aaatgaatgt
     2401 agaaaatcaa tctttatcag atattgaaat aatattatca cacaggtatg ataatggtgc
     2461 agactattgg actacacctg ataagcgttt aataaaaggt tctcctttct ctactcttga
     2521 tagtgtactt tatctattag agttaggtgt gaaccctgat gatactttac ttaaagaatg
     2581 tgctgacttg atatttagca catggcgaga agatggacgt ttcaaactat atccaaatgg
     2641 aggtatttac ccttgtcata ctgcaaatgc tgctaacgta ctctgtcata tgggatatgc
     2701 atctgacatt agaatgcaaa aaacgtttca acatttttta gatacacagt atactgatgg
     2761 tgggtggcgt tgcaataaat ttagttttgg tcgaggtcca gaaactgaat actcaaatcc
     2821 actaccaact cttaatatat taaatgcatt ccgttttagt aattatctaa ataaagaatc
     2881 aaaacttgac aaagctgtag attttctact tgaccattgg actattaaaa aaccactcgg
     2941 cccttgtcac tatggaattg gcacattatt tatgcaagtt gaatatcctt tccgtaatta
     3001 taacctattc ctttatgtct acgttttatc attttataat cgtgcaaaaa aggataagag
     3061 atttttagaa gcattgaaga tacttgaatc taaaatgata ggtaataaaa ttgtcgttga
     3121 acgtgtagtt ccaaaactat ctaaattatc tttttgtaaa aaaggcgagt caagtgaaat
     3181 agccacaaca cactattacg aaatattaaa aaacctagaa aaataatagt tatatagata
     3241 aaacatatta aaaaatagtg gctttaaatt ttttaaagtc actattctat ataaatataa
     3301 cattataaaa aatatattaa actatttact cttttttaaa accgttgatt ttcttgctat
     3361 ataa
//
LOCUS       DACTGW010000304         3361 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.307, whole genome shotgun sequence.
ACCESSION   DACTGW010000304 DACTGW010000000
VERSION     DACTGW010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.307"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..153)
                     /locus_tag="I9573_003460"
     CDS             complement(<1..153)
                     /locus_tag="I9573_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4798045.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARY"
     gene            complement(327..1193)
                     /locus_tag="I9573_003461"
     CDS             complement(327..1193)
                     /locus_tag="I9573_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT4798046.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNVEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTREYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(1419..2288)
                     /locus_tag="I9573_003462"
     CDS             complement(1419..2288)
                     /locus_tag="I9573_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HAT4798047.1"
                     /translation="MDYKTIILNRKSVREYKNIDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNKSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(2794..3015)
                     /locus_tag="I9573_003463"
     CDS             complement(2794..3015)
                     /locus_tag="I9573_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798048.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
BASE COUNT         1133 a          557 c          338 g         1333 t
ORIGIN      
        1 gtatctagct atcttaaagg ctaagatgat agaaggatta tacctaccat tttctaatga
       61 acctatagtt tgtcttgata cttgcaaaat agaagcaagt tcttcttgtt ttatgcctct
      121 ttcttttctt atttcttcta atctattttt cacatctatc cccctttaat atctcaacta
      181 cggaaagtct actttccata attatacaac tatatttcat ttatgtaaag cttgctttcc
      241 atatttacaa aaaaataaca gactattttc agcctgtaat tttaaaattt catgttgttt
      301 attaaattta atgttattca ttaactttaa cttggtttct gaattggaat ccaaacttct
      361 gtagtataat caggagcatt gatatcgcca ttgggataaa gttcaatatc tggtaagtta
      421 gcatacacat acccagaact tggtaaccac tcagtaataa ttcttttttg caattcctgt
      481 attgccagag catttggcat tggagaaaca cattcaaata ctgcccatgt acctgatggt
      541 attaagtatt cacgagtatc ttctaataca ggcttatctg ttggtgcagc aatataataa
      601 tctaaaccct tactatttgt acacacacta actccaagca atccacaagg ttcgttattt
      661 attaactctg aaagtttatc aattgttcca ttttttatgc atttagccca taacttagga
      721 actttctcaa agcactcttc tacaatcatg ttcatacttt cttttatacc aacaatacga
      781 aaccctttct tttctacaat acgatattgc atttctacat ttccttttac ataaatgctt
      841 aatgtcatct taggatatgc ttttaaaaca acaccctttg ctctagctgc agatggagaa
      901 atgttatgta tattttgaaa agctctatta aaagatgttg gtgaatcgta accatacttc
      961 tcagatagtt ctaaaatttt gatgttactg ttctgaagtt caaatgcagc cttagtcata
     1021 cgtcttcgtc taatatattc agataatggt acttctgcaa tatatgaaaa cattctttga
     1081 aaatgaaagg ttgaacaaca tgcaattttt gctgcttctg cataatccac tccactttcg
     1141 agattttctt caatataagt aatagcttga tttaatttat ttatccattc catttgatat
     1201 tacctccaag caaattatac agtttaataa aataatatac ctctctattc atgcacaaaa
     1261 ttgtaagcat ttaaattcat aagtattttt caatcaatac aataaatata aaaattgtat
     1321 tatacaataa aaaagttcaa taacatttta ttataaaata ttattgaact ttttataaaa
     1381 attaatttaa gtgtagttaa attaagtatt aaactatctt atatattaca atatcctact
     1441 atcttatagt cttctggcac tttataatct ttgtctggag tacctaaatt ccattttaaa
     1501 tcaaacatag ttgcacttat tattgttgca aaataaagca taactatacc aatgtctatt
     1561 ttttcttcat ataaattagt atgaccatct tttctaacag ctaaaactac agtgcctcca
     1621 tcaactataa atctccatgg ttgtctattt aatgtagatg gagctaatct tgcatagtga
     1681 aatgcatcaa gaagtgctct ttcttctaag atttctatac ttgcaacatt tccccattcg
     1741 ttcatataaa caacatcttc tacacctagt ctagtagacc ccttatctgt tacaacttgc
     1801 atatcagatt ttgaataatt ttgcccagtt tttgtagcat ttaatacttt agcattactt
     1861 acattttctc catatccaat agctataatt cctgttacct ctttatctga attaattcca
     1921 agtttttctt ttaccttttt tccatcttca aaagtaaccc aacaagaacc aattccaagt
     1981 tctgttactt taaagattat actttctcct atgtaccctg aattttcaat gtaattatct
     2041 tttatgtcag ataatattat aatataactt ggagaatcta tcatatttcc tttgtaccct
     2101 gctactttat ctaaattatt aaaaacttca cttttattca taattcttac atcaacatct
     2161 atttctggaa ctagtttctt acaagattct gagtatttct taatttcact aagatattct
     2221 tctttaatat ctatgttctt atattctcta acagactttc tatttaatat gattgtttta
     2281 taatccataa ttttatcctc cccaaactag tattgtctta attatatacc cattaatata
     2341 ttattttact catttattta ataaataaag attacaattt tgtaattatt atttattaat
     2401 aatattccac ttttacatta ataatgacca gttgtgcaaa atccattgcc aaccaccaaa
     2461 taaaaacata ttataaaagt aatataaaat aacaaacaaa tcttaacaaa aaattaaact
     2521 ttttttacac gaaagaaggc ttcccgttgc cttctttatt tttataaaaa aataagctgt
     2581 ttctaaaatg atttcacatt tttgaaacag ccaagttatt ctataatgac aactataatt
     2641 tgaggatgtg agggagagtt gtcattataa atttagggta attatattat gttttaagct
     2701 agttcttcct atatttatat aatacatttt atatatgaat tttatatgag attggtataa
     2761 atgatatatg aaattacata tgtttatatt atattaaatt ttattattac attttccaca
     2821 acaatactca tatgtctcca cattatttgg aatatttaca aatacaaatt ctttacagtt
     2881 ctcacaatac tttaatatag atatagttcc acaagtgtaa cattcaaata ttacacaatc
     2941 ctcattttcc tctactattt caaacttttt actatgttca ccacactctg ggcacttgtg
     3001 attatttaca ttcataaata ctcctcctta ttagtatagt tacatttcaa aaacttaatt
     3061 aatgtattct atattactta ttatatattc tataataaat catattctaa tataataaaa
     3121 caaattaaat aaaaaaaaac cataaatttt aaatttatgg attaattctt gtaatgataa
     3181 ctctatttga ggatgtgagg gagggttatc attacaatag ggtattatat tatggttttt
     3241 aagctagtct ttctacacct catatgatac atatgctata tgaattttat atgattttta
     3301 cataaatatt ttatgaaatt taaatttttt agtcattttg aaatttgtaa aaagttctct
     3361 a
//
LOCUS       DACTGW010000305         3340 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.308, whole genome shotgun sequence.
ACCESSION   DACTGW010000305 DACTGW010000000
VERSION     DACTGW010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.308"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            313..1197
                     /locus_tag="I9573_003464"
     CDS             313..1197
                     /locus_tag="I9573_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT4798049.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDKKNKIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            1399..2949
                     /locus_tag="I9573_003465"
     CDS             1399..2949
                     /locus_tag="I9573_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAT4798050.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKEKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            2978..>3340
                     /locus_tag="I9573_003466"
     CDS             2978..>3340
                     /locus_tag="I9573_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HAT4798051.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSEDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVFLVGIILSVKNKNIK"
BASE COUNT         1356 a          342 c          608 g         1034 t
ORIGIN      
        1 ttaaaacaat tgtaaatttt gacttagcta agttggtttt tttacttaaa gtattatttt
       61 taaaatgaaa atttttaagt tgcataaaac acctctttct tattaaatta ttttaataat
      121 agaattgaag ttttcttgct gcttctatat tatgttgtgc aagaattatg ttttgatgct
      181 tggaaaactg actcaataac accttagttt tcataaaaat aaataaaata aatagtgtat
      241 atgataaaat aaatacatat tcatataaat aaaaataaac aataactgtc tagcataaga
      301 aaggagcaaa aaatgaaaaa atatagattg ttagttactt gtataattgc catgggaatc
      361 ataattatag gaacatcaaa tccagttagc aatttttaca agactacaaa tttgattagt
      421 tcaggaaata tcacaaatga aaaagcagat aaagatgaaa attcaaacaa aaataataaa
      481 gataaaaatg aagacaaatc taattctaaa agtaataaag ataagtcatc agaggacaaa
      541 tcgtcagata aaaaaaataa aattcaaaaa aataaaaaac ttttaatttg tatagaccca
      601 gggcatcaag gaaaaggaga tagcaattta gaaccagtag ctccaggttc ttcctctaaa
      661 aaagcgagag tatcttcagg aacagagggt attgctacaa aaaaaccaga atatgttcta
      721 aacttagaag catctcttgt tttaaagagt atattagaat ctaaaggtta taatgtaata
      781 atgactagag agacacatga tgtaaatata agtaattcag agagagcaat acttgctaat
      841 gataaaaaag cagatatggt tgtaagaata catgcagata gtttaaataa ttcttcaaaa
      901 actggagcat ctatattgat tcctgaaaaa gatggaaaat atacagctcc tatatatgaa
      961 gagagtaata aatgtgctga atttatcaaa cagaatatgg aacaatcagg tatacaaatc
     1021 aatggaatcg ttcaaagagg tgatttgact ggatttaact ggtcaaaagt accagccgta
     1081 ttagtagaaa tgggatttat gagtaactat aatgaagacc aaatgatgtc aaatccagac
     1141 taccaaagaa aaatgatgca gtgtatagct gatggattgg atgcgtattt taaataagat
     1201 atgaatagag aaaaagttag gttggtatta ttttcctaat ttttatctct attttttatt
     1261 gtacataatg caaagaagaa gtttatttta ggttaaattg tcgtaaatat aactaaaaat
     1321 attgggaata cttgatttaa gtttaaaaag aatatattat agtatagaat gtgtaaaatt
     1381 tttttgagag gtgttgaaat gctagtagta gaaaatgtga gtcatggatt tggtgcgaga
     1441 acaatattag aaaatgtatc ctttagactt agaaaaggtg aacatattgc actagttggt
     1501 gctaatggag aaggaaaatc gagtttttta aatataataa caaagaagct tatgccagat
     1561 gctggaaata taaaatggtc ttcaagagct acagtaggtt acttagacca acatacggta
     1621 ttaagcaagg aaaaaactat aagagaagtg cttagagagg cttttaagca tatgtttgac
     1681 ttagagcaag aaatgattgc tatgtatgac aaaatgggag aagctagtga tgatgaaatg
     1741 agtaagcttc ttgaagaaac agcagagata caaactatac ttgaaaatag tggtttttat
     1801 atgatagatg caaagataca agaagttgca aatggtcttg gtcttggaga aattggtctt
     1861 gataaagatg taactgactt aagtggaggg caaagaacaa aagtacttct tactaaactt
     1921 ctacttgaaa atccaactat tcttatattg gatgagccta caaactattt agacgaagag
     1981 catataacat ggcttactaa gtaccttcaa gagtatgaga atagttttgt cttagtttct
     2041 catgatattg aatttataaa taatacatgt aatgtaatat atcatatgga aaatggagag
     2101 ttaaatagat ataaaggaaa ttatgatgaa tttgtaagac ttaatgatat aaagaaacgt
     2161 caagaagaac aagcttatga taaacaagtt gaagaaagaa aaagactaga ggattttgta
     2221 gctagaaata aagctagagt agcaactaga ggaatggcta acagtagaca aaagcaactt
     2281 gataagatgg aaatattaga aagaccaaaa gaaaaaataa aacctacttt tgcttttaaa
     2341 gatgctagag cagctagtaa aataattttt gaaacagaga atttagtttt agggtatgat
     2401 gaagcattga caaaaccact taatttccat ttagaaagag gaaaaaagat tgccttaaaa
     2461 ggaatgaatg gtattgggaa atctactcta ttaaaaactt tacttggaat aataaaacca
     2521 tttgaaggaa atgttaaatt aggtgactat ttggaagttg gatattttga acaagagagt
     2581 tcaagggaaa atagtaatac acctatggat gaagtttgga gtgaatttcc agggcttaca
     2641 aactttgaag ttagacaggc tcttgcaaaa tgtggactta caaatgaaca tataacgagt
     2701 caaatgagag tattaagtgg tggagaagct gctaaggtta gactttgcaa agtaatgtta
     2761 aaaaatataa atttcttagt attagatgag cctacaaatc atttagatgt tgaggcaaaa
     2821 gatgaattga agaaggctat caaagagttt aagggaacag ttcttttagt atgccatgag
     2881 ccagaatttt attcagaaat tgttgatgat gtatggaata tagaagattt taccactaaa
     2941 atcgtgtagt gatttaactt attgctgggg ggatttcatg aaaaagtttc taaatgtact
     3001 tttttcagtt tgtattagta ttgttataat agtaggtgtt attaatttta ctgttggatt
     3061 taaacaactt tattattttg acattgatta cttaaatata tcagagttat ctggtttatc
     3121 tgaagatgat ataaaattga actacgatta tctaattgat tataacttaa ataagaatgt
     3181 gtctgagttc aagttaccaa cattaaaatc ttctccacaa gggaagatac attttgaaga
     3241 agtcaggaat atatttcaaa atataaataa attagctaaa ttattattgg tagttttttt
     3301 agtaggaatt atattgagtg taaaaaataa aaatataaaa
//
LOCUS       DACTGW010000306         3316 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.309, whole genome shotgun sequence.
ACCESSION   DACTGW010000306 DACTGW010000000
VERSION     DACTGW010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.309"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            228..2585
                     /locus_tag="I9573_003467"
     CDS             228..2585
                     /locus_tag="I9573_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAT4798052.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKDDFLYSGSFVVSGEAFVRVENIGKDNYANKIVNDAKISKKHNSQLRNSLN
                     SILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKIRIEVEKYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDNDIYEAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2671..2988)
                     /locus_tag="I9573_003468"
     CDS             complement(2671..2988)
                     /locus_tag="I9573_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAT4798053.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1299 a          348 c          509 g         1160 t
ORIGIN      
        1 aatttatctt gccttaactt tatttactct tttatcttaa gaaaaactta actaaaatat
       61 agcgaaattc ataaaaattt aggaaatctt aagaattata atatttaaat ctttagattt
      121 atattttatt atataaacta tagaataata aaatataaaa attgagaatt ttataatata
      181 tttgtagatt tgagtataaa aaagtgctaa gttgaaagga gtaaaatatg gataaggtta
      241 atttagaaag gtatagttgt gatataaatg ttggtttaaa taaaaatcaa cttcaaagta
      301 gaatagatga taatttaatt aatgcatttg ataatggaaa aaccaaaaca tataaacaaa
      361 tttttaaaga caatatattt acacttttta atctcataaa tattgtttta gtctgtttat
      421 tagtttcagt tggttctttt aaaaatacct tatttatatt tattgctgta attaatacta
      481 ttataggagt aatacaagag ataagagcta aaagattatt ggacaatcta tctgttatag
      541 tttcaaataa ggtatctgtc ataagagaaa aagagaaaaa agagattgat gtacaagaat
      601 tagtattaga tgatattatg attttaaaaa caggtaatca aatttgtgca gattcaaaag
      661 ttttaaatgg aaaattagag gtaaatgagt ctttggttac aggtgaatct gatattatta
      721 taaaaaataa agacgatttt ttatattctg gaagttttgt agtttctgga gaagcttttg
      781 taagagttga aaatatagga aaagataact atgcaaataa gatagttaat gatgcaaaga
      841 tttctaaaaa acataattct caacttagaa attcattaaa ttcaatatta aaaattgtag
      901 gtataataat aattccttta ggaattatat tgtttgcaaa acagcatttt ggaaatggag
      961 attctatagc tacatctgta gtaggtacag tggcagctgt aaatggtatg ataccagagg
     1021 gattaacatt acttacgagc attgctctag ctgtaggaac tataaaactt gctagtcata
     1081 aaactttagt gcaagaactg tactgtgtag agactcttgc tagagttgat acactatgtc
     1141 ttgataagac aggtacaatt acagaaggta aaatgcaagt tgatgatata gtaatgttgg
     1201 aagaagtgga tattaatgaa ataatgggga atatatgtaa ttctctcaaa gatgataatg
     1261 caacactaaa tgcaataaga gataagtata atgttttaga tacgtataaa gctaagaatt
     1321 taataccatt ttcttcagaa agaaaataca gtggggttac ctttgaaaat aaaggtacat
     1381 atttattagg tgcatttgag tttatattta aggaaaaaaa taaaaaaatt agaatagaag
     1441 ttgaaaaata ttctaaaaaa ggaaacagag taatagtttt agctcatagt gattcttata
     1501 tggaagataa taatattcct gaagatataa agccactagc ttttatattg atattagata
     1561 aaattagaga tgaagctaag gaaactttag aattttttta caatgaaggt gtagatataa
     1621 aaattatatc tggtgacaat cctataacag taagtgaggt agctagaaaa gcaggtctta
     1681 aaacggctag taattttatt gatgcaacaa cacttaaaaa tgataacgat atatacgaag
     1741 cagttgataa atacagtgtt tttggaaggg taagcccaca acagaaaaaa caaatgattt
     1801 tagcattaaa aaaacaaaat catacagtag ctatgactgg agatggtgta aatgatgtgc
     1861 tagcgcttaa agaagcagat tgcagcattg ccatggcatc tggaagtgat gttactaaga
     1921 atgtttctaa tttagtctta ttagattcaa actttgcatc aatgccaaag gttgttatgg
     1981 aaggacgaaa ggtaattaat aacattcaaa gagcatcttc tttgttttta gtaaagacaa
     2041 ttttttcatt cttcttgtca ctattaacat tattcttctt cagtagacag tatccatttg
     2101 ttcctataca attatcacta attggagctg taacagtggg tataccatca ttcttcttag
     2161 ctcttgaagc aaacaaaagt agaatttcag gaaacttttt actcaatata ttaaaaaatg
     2221 ctttaccagg agcactatgt gtgacagtaa atgtaattat tgtttacaat atagttgaat
     2281 atttaggcta tagttcaagt gtattttcta caatgtgtgc tatattaact ggtgtatgtg
     2341 gtcttttaat attaagacaa caatgtttgc catttaataa agaaagatta tttttaattg
     2401 taagtatgac tattgcattt gttattggga ttctattttt aggtggatta ttctcatttg
     2461 tagcattgag tagagaatta attctcataa ctatggcatt agcaatatta atgccaatta
     2521 tgataaaagt tatgttattt gtcttggatg agatattaga gacaattgtt ccagatttaa
     2581 attaatttta aaatgatata aataagagca gttttagttg aactgctctt tttagatgtg
     2641 tttaatttta tataagaata tgtattttta ttaatcagct atatatgagc aacagtagtc
     2701 aacaacttca ttaaccttta aatcaaaact tgaatcacta ggaacattaa atttttgccc
     2761 ttctttataa gttacaaact cattgcttcc aggcagtttt acatccatac ttccagcaag
     2821 catctccatt atttcttctt ctctagtaga aaaagtatat tctcctggca acattaatcc
     2881 taaagttttt ctttctccat taggcagtat tattactctg ctacttacct taccatcaaa
     2941 ataaacgttt gccttcttta ctgcagtaac attcttaaat tcactcatta gataaaacct
     3001 ccaaaattat ttgtacatat atattatata aaaataatgt aaaaaatgct atatatttgc
     3061 aaaaaaaatt tagtatttat taaaaaaatt aataagaata aaaaagagat gaaattgctt
     3121 aaattgataa aaaatataaa ataaattgca aagtaagcaa gaaaagaata agttgaagag
     3181 ttatatatta taaatattaa aaattttagt attaataaaa aagatattac attaatatga
     3241 aaatatagga gattagaagt gtttgataat tacagtttat atattataat aaaataaatt
     3301 gttaatttta aaagag
//
LOCUS       DACTGW010000307         3288 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.310, whole genome shotgun sequence.
ACCESSION   DACTGW010000307 DACTGW010000000
VERSION     DACTGW010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.310"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            259..975
                     /locus_tag="I9573_003469"
     CDS             259..975
                     /locus_tag="I9573_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4798054.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMRLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVNIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAINTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            950..3283
                     /locus_tag="I9573_003470"
     CDS             950..3283
                     /locus_tag="I9573_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT4798055.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLL
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
BASE COUNT         1380 a          291 c          561 g         1056 t
ORIGIN      
        1 tttatatatt aattctaaat atatttaaat ctttagaaaa gtatatgtaa tatcttaagt
       61 ttttaatttt aatattaagt atataaaaca aaatgaaaaa atattaagtt taaactagaa
      121 ggaggtttct aagatggaca ttaaatacaa ataaccagaa caaaagatat aaatatatat
      181 ataaatggaa aaacttctat catattgtat aatcatatat gtaaagaaaa tttataaatt
      241 aatataaagg agattaaaat gaattcatat aatattttgg tggtagaaga tgaaaaggag
      301 atcgcagatg ctatagagat atatctatta aatcaaggat ataatgtttt taagggatac
      361 aatggattag aaggtcttaa ggttatagaa aaccaagaaa tacatttagc tatagttgat
      421 ataatgatgc ctaaaatgga tggtataaca cttacaatga ggcttagaga aaactataat
      481 tttccagtaa taatgctatc tgcaaaatca gaagaagttg acaaaataat ggggcttaat
      541 ataggagcag acgattatgt aacaaaacct tttaagccat tagaactatt ggctagagtt
      601 aattcacaac ttagaaggta cactaagtat ctaaacatgg ttggaaataa agagcaaaag
      661 gtagaaagtg atgatagtgt gtttgcaata ggcggattag aattaaatga aaatacaaaa
      721 gaagttaata ttgatggaaa atatataaaa acaactccta tagaatttaa aatactaagt
      781 ttacttatga gaaatgcagg aagagttttt tctgctgatg aaatatatga aagagtttgg
      841 aatgaaaatg ctataaatac agatacagtt atggtacatg tgaggaatat aagggaaaag
      901 atagaaattg acccaaaaaa tcctaaatat ttaaaggtgg tgtggggtgt tggatacaaa
      961 attgaaaaaa tttaaagata aatatatagt tactattata gtttttatac ttatgttatc
     1021 agcatcatta ggtatgatta gtcaatattc tactatacaa gcaggtgcta aaggtggagc
     1081 aaagaatcca tttgaacagg aaagctttgt agatagtata tataaaggaa gttatgtttt
     1141 atatcataat atgaaggaag aacaagaagg taagatgata caaccatcaa agctatttat
     1201 caatgaagaa actatgaact atataaaaaa tgtaagtaaa gaaaaagata cttatgatgg
     1261 ttcagaaact tattatacag atgatagtat taatgcagat tttaatgaaa actttgaaaa
     1321 ttgggagcat ttcattaaag cgtcaggaaa gaatttaaag tactatttag ttgataaaga
     1381 aagcaatttt gaatttttta atgataataa agaattagga gtacttgata caacaaagaa
     1441 tgatgcttca aaagaaaatt ctgatgaaaa tcagtcaaaa aatacagaaa tgaatgatga
     1501 ggaaagacaa aagcagatta cagatataaa ggaaaactat agattctacg ttgtaatgaa
     1561 ttttgataaa gatggaaaag taaatattgt ggattcttat ggagtaaatc aaaaagttat
     1621 taatcaaatg ttgttatcta aagcaagaga agatttaatg gatacaagtt taggaggaaa
     1681 ttcaatttca tataagttat ctccaataaa aaatataaca ttagtatatg ctgtacctaa
     1741 agtaataagt aaaggtgata gtgtagagta tggttatgga aatacttatt ttgatgatgg
     1801 tataatttca tatataagca atgtagaaaa taattcttat tataagattt ctggaatgat
     1861 tttaaaaatt atattagcta tagttgttat tgtagcttta gcattcccat atagaaaatc
     1921 taaagagttg ctaggatttg atacattatt aagaataccg ttagagatac tctttattgc
     1981 tcttgctatt atacaggggc ttgtagattt atatcctact gttataataa gtaatacctt
     2041 gagtgggaat tatgtagagt accttataaa caatgacata agtgctaaaa tggctagcat
     2101 attggtaagt ttaatgaatt taacttattg gtttgtaata ttctcaataa tatttgtatt
     2161 tataatactt ttaaaacata ttttaaatgt tggaataaga gagtatttta caaaacatac
     2221 tgtgacagga atgttttttg tatggttaaa aaaagcagga aaaacagtta ttaatcaaat
     2281 tagaacaata aatctaaaag aaaagccaaa taaaactata gttacaattt tgggaataaa
     2341 tttattgatt atagttataa tgtgtagtat gtggttcttt ggaataatac tcgcattaat
     2401 atattcagta gtattattta agatactgtc agattactct aaaaagacaa tatatgaata
     2461 taatcaactt ttagatgtta caaagaaaat ttcagatgga aacttagatg taaatatgga
     2521 aaaagattta ggatttttta atcctataaa agacgaatta ggaaatattc aaactggatt
     2581 taaaaaagct gttgacgaag aggttaagag tcaaaagatg aaaacagagc ttatatcaaa
     2641 tgtatcacat gacttaaaaa cacctctaac atctataatc acttatatag atttattaaa
     2701 agatgaaagt ataacagatg aaaatcgtaa gttgtatatt gatacattag acagaaagtc
     2761 acaaagattg caacatctaa tagaagattt gtttgaagta agcaaggcaa atagtggaga
     2821 tgtttatttg aatatagtaa atgtggatat tgtatctcta atgaagcaga cacttttaga
     2881 attagatgac aaacttgcag attcatctct aatagtaaag aataactttt ctaatgaaaa
     2941 aataatatta ccattagata gtcagcgtac atttagagta tttgaaaatc ttataataaa
     3001 tataagcaaa tatgcaatgc caaattcaag agtgtatatt gatattttag aaacagataa
     3061 taaagtaaat attatgctta aaaatatgtc tgcatctgag attgatttta gtgtagatga
     3121 tataatggag agatttgtaa gaggagataa atcacgtaac acagaaggtt ctggtcttgg
     3181 tttagcaata gcaaaaagtt ttatagaatt acagggtggt aagatgaata taagtgtgga
     3241 tggtgattta tttaaagtta caattacttt taacaaaaat tagattag
//
LOCUS       DACTGW010000308         3241 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.311, whole genome shotgun sequence.
ACCESSION   DACTGW010000308 DACTGW010000000
VERSION     DACTGW010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.311"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(200..577)
                     /locus_tag="I9573_003471"
     CDS             complement(200..577)
                     /locus_tag="I9573_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT4798056.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
     gene            complement(764..1735)
                     /locus_tag="I9573_003472"
     CDS             complement(764..1735)
                     /locus_tag="I9573_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAT4798057.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            complement(1769..1966)
                     /locus_tag="I9573_003473"
     CDS             complement(1769..1966)
                     /locus_tag="I9573_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAT4798058.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            complement(2034..3218)
                     /locus_tag="I9573_003474"
     CDS             complement(2034..3218)
                     /locus_tag="I9573_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005977325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HAT4798059.1"
                     /translation="MEENAPCYIYDETNIIEKCKELKNNILGFDFLYSIKTNPYNKIV
                     KSIAEQGFGADASSTNEVMISSECGVSKYDIYYSSPGKTEIDIETVLDKCIIIADSIT
                     EIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVFKMLSMCDYV
                     SVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSGLGVAYDNLR
                     ENPLNLKELSKLTQKIVFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVDIKVSRGKKY
                     LIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNLKEEEYVNVV
                     GNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPKQYYKRKDGY
                     IE"
BASE COUNT         1134 a          525 c          349 g         1233 t
ORIGIN      
        1 aaattttaaa aatcatttct tatttaaata agtggtatca tttaacttga tactccctat
       61 tccaattatt ttaggtattt tactaattag agaacctatt taatattact ttatgaatag
      121 tgagtagctg attaaataat accatttatg gttttcagcc atagattaat taaattctta
      181 gagttttatc ctgatataat tactcttctt ctaaattttt aaaatgactt tcaaataaat
      241 ccaacatatc tttatcaaca acttcatcat catgtagtgc tgaaattaaa ctctgtaaag
      301 aattgtcgtg tatattgctt aaaaaatctt ttgtctcaac acttaaatac tccttatgtc
      361 caacaactat tgtataatga gtgtatctat ctattttttc tgcacttaaa aaatgttttt
      421 taacaagtct tgataaaagt gtcaatgtag ttgtttgttt ccaaccatat tttcgttcca
      481 tagcttcaat agtttctttt gatgttactg tatcattttt ttcccaaata aacttcataa
      541 cttttaattc tgcttgtggt atttttgaaa ttgtcataac cttacgcccc tctcaaactt
      601 ttattaaatt aatactatcg caatatagtt tttaagtcaa agattttgtc gataataata
      661 ttttccaatt tttaccatag agttattttt tatataattc tatgataaat tacataaaaa
      721 atatttattt tacatcataa aattagaaat gtaaatagta tgattattta gtcttatctt
      781 taataaacaa tccagtaaat ttatacatcc aaagtgcatt cataaacaac aataaacttg
      841 ttgaaaaaaa tactgttcta atacctaaat gtgctgatat ttgtccccct aaaaatgctc
      901 ctgatgaaca acctatatac tgggcagatg cattatatcc aaatattttt cccacatatt
      961 cttctggtgc aattttttta agcaagctgt taacagaagg attaagccct gcaactgaca
     1021 accctaataa aaatctcaac aacatcagtt gccatgcagt ttttacaaaa gcttgtggta
     1081 taaatataat cccagcccat aaaagtgaaa ttaacaatac tttttgagga cctattttat
     1141 ctgacatttt tccaagtttt tgagctgcta ataaatttgc taatcctgat gcagaaaatg
     1201 tcaatccagc tattaaagct acatttgatg caaaatttgt aagttgtgat atatatatgg
     1261 ttacaattgg ttctatagag tataaagcta gttgagtaat gaatgtggtt acaaaaatag
     1321 atgcaattaa aaatttattt ggcaatatat ttaattgttc tttaggtata gaaattgatt
     1381 cttttatttc tttaggttta aaattatcta ctacaaataa aatagagatg ataaatgata
     1441 caaacaacaa tcctcctgtt ataataaatg tacttttaag acccaagatt gattctatat
     1501 atcctcctac tgttggacca attaatgagc cagtagttga tgcagttgca agagtaccca
     1561 aagcccatcc tgaatgattt ttatctgtct gtgttgcaat aagagtagta catgcagtag
     1621 cataacccgt aaaaactcct tgaagtattc ttaatcctaa taactgataa acattttgaa
     1681 caaacgccat ggtaaatata actattccca tcccaatact tgcacgtaaa agcattgatt
     1741 ttcttccata tttatcagca gctttccctc aaattggcga aaagaatgct gctactacaa
     1801 aggtacatcc aaaaattata cctgaatact gttgtattaa actaacatca gttaccccca
     1861 actgtttaat atataatgga agtataggtg ctatttggct catccctata gaactaaaaa
     1921 acatacctat ccaacataca aataaatttc ttttccacaa ttccatatag aattacaccc
     1981 cctgcatttt aaattttttc aataatacta agtttattaa tttatcctat tgtttactca
     2041 atatatccat cttttctttt ataatattgt ttaggaaatt cataactcga aaataatagt
     2101 ggtgataaag aataggagta acttccagca ttactaattg aaatcaaatc tcctatattt
     2161 gcttttttca atttaacatt atttgcaatt acatctaatg atgtacagag attcccaaca
     2221 acatttacat actcttcttc ttttaaatta tttaatactg aaagagaaaa ggcatgattt
     2281 gatgtatata atggctcttg accttctaat tctactccac aagtaatatt ttcaattaaa
     2341 ttagaaataa taggtctcat aaatccatta agtccatttt ttacaataag atattttttc
     2401 cctcttgata ctttaatatc tacaatttta acataataat tacctgattt acaaactatg
     2461 aaacgtcctg attcaataag aagtcttgat ttcagtgtgt ttgaattagt aaaaacaatc
     2521 ttttgagtta attttgaaag ctctttaaga ttaagtggat tttctctaag attatcataa
     2581 gctactccaa gtccactacc aaaatttata aattcaatat taatatactt acttttattt
     2641 aaatttaaag ctataaaaaa acatttttca taataatgtc ctaacttttc aaaatccaat
     2701 acctgagatt taatatgtat atgaatccca actacagata cataatcaca catacttaac
     2761 atcttaaaaa cactgctcat atcttctata tctataccaa atttgcttga tttagcacta
     2821 tcatcatcca tagtaaaatc aggatttatt cttattccta ttttaacttt ctgattatgc
     2881 tttttagata tcttatttat aagttctatt tctgtaatac tatctgcaat aattatgcat
     2941 ttatctaaaa cagtttctat atcaatctct gttttgcctg gtgatgaata ataaatatca
     3001 tatttagaaa ctccacattc tgatgaaatc ataacttcat tagttgaaga agcatctgct
     3061 ccaaaacctt gctctgcaat gctttttaca attttgttat atggatttgt ttttatagaa
     3121 tataaaaaat caaaacctaa aatattattt ttcaattcct tacatttttc aattatatta
     3181 gtctcatcat atatataaca tggtgcattt tcttctataa atttatagtc ttcatttgag
     3241 t
//
LOCUS       DACTGW010000309         3193 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.312, whole genome shotgun sequence.
ACCESSION   DACTGW010000309 DACTGW010000000
VERSION     DACTGW010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.312"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(278..499)
                     /locus_tag="I9573_003475"
     CDS             complement(278..499)
                     /locus_tag="I9573_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HAT4798060.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            1079..1336
                     /locus_tag="I9573_003476"
     CDS             1079..1336
                     /locus_tag="I9573_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAT4798061.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            1785..3023
                     /locus_tag="I9573_003477"
     CDS             1785..3023
                     /locus_tag="I9573_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798062.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKRNKSATKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1321 a          289 c          480 g         1103 t
ORIGIN      
        1 ttttaattgt tgactctagt tattgctgat atagaaaata tttaatttat ttcaattaat
       61 tagattaaag tagggtagca tttaaaatag ctaacatgtt taagttgaaa tacgtttaat
      121 atattaattt ttcaaagcat taatatatta aagttataac caaaattgaa gttataatct
      181 gccaaatcta tagcgtatta agtgcgttat ataacttaat cttttatata aagagttaat
      241 ttttttaatt tgtaaagcag agtacgttta aaagatacta tttagataat gatgtactat
      301 ctaattttaa aatatctaca atagggttct cttgaagact tgctattgct tctgcaacag
      361 cataaacatc ttcatttaat gcctctgctt ttacatttgc aaatgattta cttttaacaa
      421 ctgccttacc cttatcatca aaaccagcat ccaattttag ttttatacta gataaatttt
      481 ttgttgaaac aacttccata aaattacctc ctaaaaattt aatttcaaag atttgtcgac
      541 tttctttgtt agctataata ctatcatcta agataatatc ggagttaaac agtaatttct
      601 aaagattttt tgtgtaaatt tttataggaa aaattttact tttctgatga tgaaaaagtt
      661 tacaagttat ttttgaagat atgttcttag tgttttgaaa tagtatttgt atatattcat
      721 gcttagttga gatagttttg tatggatttt catatgtgta gagttaaatg ttaggtacca
      781 aagtttatgt taaattaaat gttaaagctt atgttaaaaa ttttattaaa tttaatctgt
      841 aaataaaaaa gttaaaagtc caatattaac attttacttt taaaccatta aaatctttat
      901 taaagctaac aaagttataa gttatgagaa attttattaa agttaataca aaaattaatt
      961 ttaaagctaa gaaatttttt attagattca ataaaactaa taaatatagt ttagtcaata
     1021 atgtattaaa taaaaacttt gttaagtcta taaaaatatc ttaaaggagg tgtaacttat
     1081 gaagataaaa gtggaggata taccagttca atttcatgca atggtagaaa taatcggaat
     1141 tgataagttc atagaggttg ccaagttgta tggaggaaca aatacttata tacctacgta
     1201 taaggggctt tttagatatg ctagaaatag ggatatagta aaacaattta atggagtcaa
     1261 ccataatgaa ttggctatta gatataatat gtgtgtaagc aatattaagc gaatactcaa
     1321 tgaaaataat gtttaaacat aatgaaaata gccttgttaa atgtaagagt aaagacaaag
     1381 aagatgtttt ataaagtttt tttagacaaa ttaactaaat ttactactta ttaatttaaa
     1441 tacattgtat attctataaa aaaatttgtt aaatactgga gatatattga gaatacaaag
     1501 aaaatggtcg aaaatatttg ttggctaaaa aatagataaa atgatacaat tgcttgtata
     1561 aaggggaaaa ataaataaat aaactatata aataaagaaa tagcaattaa tatgacataa
     1621 tatatttata aaattaagaa tataaataga tattttaagg gattagttgt taaattagga
     1681 aatattaaaa aaatatatat atttatattt aaactatttt gttatttata taaaaatact
     1741 tatctagtta ctagcaatta ttagtagaaa tgggggaaat agagatgaat aagggatttt
     1801 acaaattaaa ttcagtggta ttgaactttg atattaagaa tgatgttaac aagataagat
     1861 ttaatcatta tataatgaga aaagaaaaca tacaaagact tcaaggaact ctgccaaacg
     1921 gacaatttta tatgacagtg agaagagctg ctacagattt acaactttct atttctacta
     1981 taagtaggct tgtaaaagag tttttggatt tagggataat aagattagtg tctaagggtg
     2041 taaaaggtaa tcgttctgta tatagttacg taagtataga gtttaatgga agtttatttg
     2101 atgatagcaa atatatggaa cttgactgtg tatgtaatga tgaatttatt gataatagtg
     2161 gcatagagtt tgattgtgat agcaaagata attcttttaa tggagatata aatgtagaaa
     2221 ctgtgcattg cattaatgag tatttctctg atgatgatgt agagcataat tataacaatt
     2281 tccataataa agatactata aaggataaag ccatttctaa tattagcact atcaaaaaat
     2341 atattgatga atcttttgat acaaataaat cagaaaattc taacttacaa caggagtcaa
     2401 taaatttcaa ctttaatgat acatgcaatg aaatagataa aagaacgaat aatagtatag
     2461 aaaataatca aaataaaagg cctatgagat tgagtgacat cagtgtttta agtgataaag
     2521 gtatgacaaa taaaaagaat tactttaatc ataatcatgg tatacaaaaa aggaataaat
     2581 ctgcaacaaa gaaaaaagaa tccttaaata aatttaataa aaaaaatata cagcaaagca
     2641 ttataaatat gctaaataaa aaatcaggta agaattttaa aatcgattct ccaataagca
     2701 taaaacttat caatgaaagg ctaaaggagg gctatgtctt agaagatttc tataaagtta
     2761 ttgaggtaaa agtggctaag tggaaaaata ccataatgga aatgtatata agaccagaaa
     2821 ccttgtttag ctataagttt gaatcctatg taaatgaaaa catatctatg aaaagtcaaa
     2881 gtaatgttca tccatgtaaa gaaggcaata gtaatgatag cagtaattat atacattctg
     2941 attatacaac atatgatggc agtgtgcgtg agagaaaaaa atatgagaat gtaagatact
     3001 ggaatcctat atgtgaaatt taattagttt aagaatgtat ttatgataaa taagatatta
     3061 atagagaata gtgagctata gcaaatgata tatcaatagt tggtagtaag ttatattaag
     3121 caagatatta ctatcaggta gtgaattata gtgaacgata tatcaataat tggtagtaag
     3181 ttatatcaaa taa
//
LOCUS       DACTGW010000310         3192 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.313, whole genome shotgun sequence.
ACCESSION   DACTGW010000310 DACTGW010000000
VERSION     DACTGW010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.313"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            278..1546
                     /gene="eam"
                     /locus_tag="I9573_003478"
     CDS             278..1546
                     /gene="eam"
                     /locus_tag="I9573_003478"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HAT4798063.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(1639..1995)
                     /locus_tag="I9573_003479"
     CDS             complement(1639..1995)
                     /locus_tag="I9573_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798064.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(2146..3144)
                     /locus_tag="I9573_003480"
     CDS             complement(2146..3144)
                     /locus_tag="I9573_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAT4798065.1"
                     /translation="MISLLMSLLTPFIIAFVLAYIFNPLVKFLESKLNFKRIYSLLLT
                     YGVLIVILISFILFTVPSIVNSLADLVAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKE
                     YGDKIMSVMPKFSNLLIGSLGGIFSTTFSVGKFIVQFLLGFIICFYILLEKEKFLLFT
                     KKVVYISLGKKYGDFIIELCQSLNLNIGKYFTGKILDSFIVGVLSGIGLYFLKSEYAL
                     LFGTLMGVMNMVPYFGPVIGMAPVVIINLFSNPTIALTSLIYLIIIQQVEVTFIEPKI
                     VGGQLGLSPFFTILAVTVGGGFFGIPGMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         1304 a          419 c          468 g         1001 t
ORIGIN      
        1 tgaaaatata atcgttttat ggtaaaataa ttcttaaaaa tagaaaaaat taatcatttg
       61 ttaaaaaaaa tgcataattt attgtaattt attgacgaag gttgaaaaat attcataaat
      121 gtgcgaaata acagaaaaaa tgcaaaaatt aattcaattt catttgttat aaaatctata
      181 tggcaaaatt aaatctgcct atcagaatga tataaacaca tcagaaagga ggacgctatc
      241 aaataataca agtagttaac tttgatagca aaaaattatg aatgaacaaa ctagaatatc
      301 cttagagaga gctgctgaat taaaatcaaa aattgatgat tatattcagg ctagaaaaac
      361 gattaacaga ggtcttgaaa aagaagaaga gataaataaa cgaaaacaga aaatattaag
      421 tatcttaaat ggaactgaag aggattggaa taactacaaa tggcaattat caaatagaat
      481 aacagatgta gatactttat caaaaattat aactctaact aaaaaagaaa aagaatatat
      541 aaaagaggtt ggtactcaat ttagatgggc aatatctcca tattatttga gtcttataga
      601 cccagaagat atatgtgacc caataaaatt actgtctata ccaacacata tagagttgga
      661 agatgagcaa gaagatttgg acccaatggg agaagagtat acaaacccag caggatgtat
      721 aactagaaga tacccagata gattaataat aaatgtaaca aatgagtgtg ctatgtattg
      781 tagacactgt cagagaagaa gaaatattgg acaacaagat tctcataagt caaaagctat
      841 tatccaagaa tctatagact atatcagaga aaatgaagaa ataagagatg tactagtaac
      901 tggtggagat gctcttacat taaaagatga ttatttagag tggattctta gccaacttaa
      961 agagatacca catgttgatt atgttagatt aggtactagg actcttgtta caatgccaca
     1021 aagaattaca gatgaatttt gcaatatgct aaaaaaatat caccctgtat atataaatac
     1081 tcattttaat catccaatgg aaataactaa ggaatctaaa gaagcttgtg aaaagttagc
     1141 aaatgcagga gttccattag gaaatcaggc agtattatta aatggaataa ataatgataa
     1201 atttgtaatg agatgtttaa atcaagaatt actaaaaata agagtaaaac cttattatat
     1261 attccaaagt aaacatgtaa agggaacaaa acatttcaat acatcagtag atgatggtct
     1321 tgaaatcatg gagtatttaa gaggatatac atcaggaatg gctataccaa catatatagt
     1381 aaatgcgcca aaaggaggag gaaagactcc tttgcttccg caataccttg tatcaaaagg
     1441 aacagattac gttatgctta gaacatggga aggaaaagtt ataaaaatgg aagatgaacc
     1501 tgctgtagat ataaagaaac ttataaaaga gcaagcacag gattaaaaaa gtatttaaag
     1561 tttataaaaa gaaacaactt taaaataaaa agaaaatgga tgtcaaattt atatcaattt
     1621 gcatccattt tttattagtt atttattatt ttgttgttga ttaactaatt caagatatcc
     1681 acttgggtgg aagtaatata tctcagaagt attttcattt acaaagtaac caatatcacc
     1741 atctgcatct ggtaaaaata cataaccatt aagattttta gcttttaatg cttcaaaatc
     1801 actagcattt ccttgatata tgtaatcaac ttcattgttg tattttacta aaagttcttt
     1861 agcttgttct tgagtcaatt tagtattttg gcttacagga acattctttt gagaatctgc
     1921 ataagaagat tcatttactg gtgcagttaa taacaaacaa gataaaacta ataaagatat
     1981 aagtgctttt ttcatactaa aaccctcttt catgtttaat tatataagat tcattactta
     2041 ataatattgt atgtttccta taattaaata ttaccattaa agagaataat attacagtta
     2101 cacagctgta acttttgatt acaaaatcgt aattaaattg tatttttatt ccatttgtat
     2161 tttatcatgt ctacgattta caaactcaca gaagtatatc tttataacgc ccatcacagg
     2221 agcagataga atcataccag gtataccaaa gaatcctcca ccaacagtaa ctgctaggat
     2281 tgtaaagaat gggctaagac ctagttgtcc acctacgatt tttggctcta taaatgtaac
     2341 ttcaacttgt tgaattatta ttaagtaaat taaagaagtt aaagcaattg ttggattgga
     2401 gaataaatta attattacaa ctggtgccat acctattact ggaccaaaat atggaaccat
     2461 attcatgaca cccataagtg taccaaataa tagtgcatat tcagatttta aaaagtaaag
     2521 accaattcca gaaagtactc caactataaa agaatctaaa atcttacctg taaagtattt
     2581 accaatattt aaatttaagc tttgacatag ttctattata aagtctccat attttttacc
     2641 tagggaaata taaactactt tttttgtaaa cagtaagaat ttttcttttt ctaataatat
     2701 atagaaacaa attataaaac ctaaaagaaa ttgtactata aattttccaa ctgaaaatgt
     2761 agtgctaaat atcccaccaa gtgaaccaat aagcaagtta ctaaatttag gcattacact
     2821 cataattttg tcaccatact cttttaatgt attagggtca actgattgta aactcttacc
     2881 taagtcgaat aagaactgct ctgttttatc tatgtatgta ggaatttgag caactaaatc
     2941 agctaaacta tttactatgg atggaactgt aaataatata aaggaaatta gtataactat
     3001 gagtacacca taagtaagaa gtaacgaata tattctctta aaatttaatt tactttctaa
     3061 gaactttact agtgggttga atatatatgc taatacaaat gctattataa atggtgttag
     3121 tagtgacatt aataaactta tcactccaaa aaaatatttg taattatcaa taactttaat
     3181 caagatatat ga
//
LOCUS       DACTGW010000311         3172 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.314, whole genome shotgun sequence.
ACCESSION   DACTGW010000311 DACTGW010000000
VERSION     DACTGW010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.314"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            182..1228
                     /gene="recA"
                     /locus_tag="I9573_003481"
     CDS             182..1228
                     /gene="recA"
                     /locus_tag="I9573_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAT4798066.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            1404..2945
                     /gene="rny"
                     /locus_tag="I9573_003482"
     CDS             1404..2945
                     /gene="rny"
                     /locus_tag="I9573_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAT4798067.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNVERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         1331 a          327 c          645 g          869 t
ORIGIN      
        1 cattttaaga atattttctt ttgtgtatat aattattaat aatggtttat aaataaaatg
       61 taatgaataa aaatataatc attagtagcg tttttaaatt gactaggttt gatttatgtt
      121 atataattaa tatataacaa gagaacaaat gtttgtagat aaatgaaggg atggtataga
      181 gatgagtgta gatcaagaaa aattaaaagc gttgaatgaa gctttaggta aaattgaaaa
      241 agattttggt aaaggttcag taatgaaatt gggagaagca acatctatgt ctatagatgt
      301 tatatcaaca ggagcgattg gtttagacat agctattggt ataggaggtc tacctagagg
      361 gagaatagtt gaagtatatg gtccagaatc ttctggtaag actactgttg cgcttagttg
      421 tgtagcatca gctcaaaaag atggaggaat agctgcattt atagatgcag aacatgcact
      481 tgacccagta tatgcaaaag ctttgggtgt ggatgttgat aacctaataa tatctcaacc
      541 agatacaggt gaacaggctt tagagatagc agaggcattg ataagaagtg gagcgataga
      601 tataatagta atagactcag tagcagcatt agttccaaag gctgaaatag atggagatat
      661 gggtgattct catgtaggtt tacaagctag acttatgtca caagcactta gaaagttaac
      721 tggttcaatt aaaaaatcaa attgtgttgc tatatttata aaccagttaa gagagaaagt
      781 aggaataatg tttggaaacc cagaaactac tactggagga cgtgcactaa aattctattc
      841 atcagttaga ttggatgtta gaaaaataga tacaataaaa caaggtgata aagttatagg
      901 tagtagaact agagttaaag ttgttaaaaa caaagtagca ccaccattta agcaagctga
      961 atttgatata atgtatggag aagggatttc aaaaattgga gaccttttag atatagctgc
     1021 tgatgtagat atagttaaaa aatcaggttc atggtatagt tacaatgata ctaaacttgg
     1081 acaaggaaga gaaaatgtta aaaaattctt ggaagataat ttagatttaa ctactgaaat
     1141 agatgagaaa gttagagcat tttacaattt aaatgaagaa catgaagaat caggtacttc
     1201 agtatcaaaa gaaattgtag aagaataagt aaaaatacaa aaaaataact agtaattaaa
     1261 gacatgccac gctccctatt agcttgacac aatgttgaaa aaattataaa attaagtgag
     1321 gataaaatat ctaaatttaa ttttaattat taggaatatt ttttaatcat agtgatgaca
     1381 ttttgggatt attaacgaag gaggtggatg tcatagatag tgtagtaatg attgtaatag
     1441 gtgcagctgt tggtattata gctggatatt ttgttagaaa aaatatatct gaagcaaaaa
     1501 ttggacaagc tgaaaacttg gccaaagaaa taatagataa agcacaccac gactcagaaa
     1561 cagtacaaaa agaaaaatta ctagaagcta aagaagagat ccataagtgg agaacagaag
     1621 ctgaaagaga aaacagagaa agaagaacag aagtacaaaa gtatgaaaaa agagtagtac
     1681 aaaaagaaga agtcttagat agaaaattac aaaatttaga atctaaagaa gtaaacctaa
     1741 gtgaaaaatt aaaggtagtg gaaaagaaag aagaagaagt cgaagtaata aaaactcaac
     1801 agttagaaaa attagaaagt atatcaggaa ttacttctga taaggccaaa gaaatcatct
     1861 taaccaatgt ggagagagat gtaagacgtg aaatgtctat aatgataaaa gagatagaaa
     1921 gtcaagctaa agaagaagct gataagaaat caagagaaat tataggatat gcaattcaaa
     1981 aatgtgcagc agatcacgta gccgaaacta ctgtaacagt agtaagcttg cctaatgatg
     2041 aaatgaaagg tagaataata ggtagggaag gtagaaatat aaggacttta gaaaccttaa
     2101 caggtataga ccttataata gatgacactc cagaagccgt aattttatca ggatttgatc
     2161 caataagaag agaaattgca agaattgctc tagaaaaatt aatagcagat ggaagaatac
     2221 atccagctag aattgaagaa atggttgaaa aggcgagaaa agaagttgac aatattataa
     2281 aagaatatgg tgaacaagct gcatttgaaa ctagtgtaca tgggattcat ccggaattaa
     2341 taagattatt aggtagactt aactatagaa ctagttatgg acaaaatgta cttaaacatt
     2401 ctatagaagt tgctcatata gctggtataa tggctgctga aataggagca gatattagat
     2461 tggctaagag ggcaggttta ttacatgata taggtaaggc tgtagaccat gaaatggaag
     2521 gtacacatgt tgaaataggt atggatttac ttagacgtta taaagagtct aaagaagtta
     2581 tacatgccat gagtactcat catggagatt acgaacctca aacagtagag gctgtattag
     2641 ttacagcagc agatgcaata tcagctgcta gacctggtgc gagaagagaa actttagaag
     2701 catatataaa gagacttgaa aaattagaag aaatagctaa ttcatatgaa ggtgtagaaa
     2761 aatcatttgc tatacaagct ggtagagaaa taagaataat ggtaaaacca gaagctataa
     2821 atgatgaaga aatacatcta ttggctagag atatgactaa gaaaatagag gatgaacttg
     2881 aatatcctgg acaaataaaa gtaagcataa taagagaaac aagagctata gagtatgcga
     2941 agtaaaagaa ataatatcta aataataaga aaatattttt agaaatttaa atttgccaca
     3001 agatatgata atatatcttg tggcttttaa attgtttaag tttaatttta tataatgcaa
     3061 aaataatcta agtgctaaaa gtgctactta caaaagatta ataaaataaa gctttatata
     3121 tgtattattt atataattat ccaagtttgt ttgatataat gaagttgtaa aa
//
LOCUS       DACTGW010000312         3129 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.315, whole genome shotgun sequence.
ACCESSION   DACTGW010000312 DACTGW010000000
VERSION     DACTGW010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.315"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(90..383)
                     /locus_tag="I9573_003483"
     CDS             complement(90..383)
                     /locus_tag="I9573_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798068.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
     gene            complement(867..1070)
                     /locus_tag="I9573_003484"
     CDS             complement(867..1070)
                     /locus_tag="I9573_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798069.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
     gene            complement(1121..1372)
                     /locus_tag="I9573_003485"
     CDS             complement(1121..1372)
                     /locus_tag="I9573_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HAT4798070.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            complement(1388..2458)
                     /locus_tag="I9573_003486"
     CDS             complement(1388..2458)
                     /locus_tag="I9573_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HAT4798071.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            complement(2487..>3129)
                     /locus_tag="I9573_003487"
     CDS             complement(2487..>3129)
                     /locus_tag="I9573_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HAT4798072.1"
                     /translation="ENDYISIDDFISKNETLKDSNFLASTLNNNYSDKEIVSSDEYIL
                     GKSYDRFLTLLNKLKFIKDESMSPLSRIERALLDDFRYEKNIEGRLELPDVWHHGGPC
                     IPGAFRIFVTTDGEFFPCERVSESSKMGMIGNLDSGVDIEKAEKLLNIGKLYEEQCSD
                     CWAYSYCDICIAKIDDTKCNENEIKPHDCDRIRHSTENNMKDYCVLKSLGYQY"
BASE COUNT         1121 a          517 c          239 g         1252 t
ORIGIN      
        1 aaaattgtag ataaaactat tttataaaat ttatctacaa tttttttata taaacatata
       61 tcaaaatcaa aatattatta atttatactt taaagattcc catctttatc atattttatt
      121 cttataagaa aatataatcc tattactact aactctatca ctaaaaatac tccataggta
      181 agaattgaat caatactatc taacccattt ctaaaaaaaa ttaatgctat acctgctgct
      241 ataatcccta tagcattttt tcttactgca ttttgcccaa ctccataaaa acctggcatt
      301 tttcctttta tttcgacagc agttaaaatt atcccaaaca ctataaataa tactccaata
      361 agcatcataa tcatactaat catctaatta cctccttcac ttaaggaaat ttcaatttct
      421 aaaatctaaa ataaataata tttatttttt ataataatat tgtatatcca attaactatt
      481 ttggaaatta gtaccaataa ataatgttta aatattgaaa taatatatta attctaatca
      541 atattattaa tatatataca tttttacaaa tatatttaaa aactttactt ttaattctta
      601 ctatttaaat aagtataatt tattataata acttttaatt aatttatact tttaaattgt
      661 aaaaatcacc cctaatcact atatttacca ctataataat tgaagaggtg atttatataa
      721 cagatactat ttatgcagtt attataaaat cacactctta attataatca actgcatatt
      781 tttataataa aaggaaataa agtaagaaag ataaatttaa tcaaatatgc taaatcaatt
      841 taatagtata cttattactg aaatatttac attccatcag gagttctttg accatgtatc
      901 aatttattat aatatgtatt atatgatcct cctcttgcat tataatcatt attcttgcag
      961 ttacaagatg aagatgccat catcttacaa gtataaggac acataatagt acaaacacct
     1021 cttgtctcaa cacctatatt attcatttct attctttttc caaaactcat tcatatcctc
     1081 cccttttcta taagatcact tactttatac ctttattaac ctataccata ttgattattt
     1141 ttagaacatt atcaaaactc gtaaattgcc tattcaaaag tttttcctct ggaattttta
     1201 tattaaagtt ttctattacc tcgcaaaata ctaaaagtaa ttctcttggc tgtatatgta
     1261 aagacggtga aaaaaataat tcttttttta aattgttatt tttattaaaa ttaattccag
     1321 tcacatcaaa aaatatattc tctagtagta attccttttc agtgtaattc ataattaacc
     1381 tccaattcta aatagatatt actttagcat actcttttaa ttgattcact atattgtcca
     1441 ctaattctag taattgttta ttatcatgta taaaaaatgt tttttcataa tcttctatat
     1501 cttcaacaaa aaagttttca tctaatgaca tatatgaaaa tttttgcttc acctctgtct
     1561 catatgtatc tataactttt ttctctatac taaaataatc tatatttata ccatgtcttc
     1621 ctttaataaa atatttaagt tcttcacaat attctctatc atatatactt gatggaatac
     1681 ataatatacc acaatcattt attactgcat tagaaatttc aaaagctcta attccaaaat
     1741 taccaaccat ttttctagag aaagggaata attctcctgg aacttctata attattaaat
     1801 ctggctttgc ctctacctct ttatctttta taaaatggtt aaaattaata atctttgtct
     1861 tactacttat cgaatcatca aacataaact ctggtatagg aatcatttta aataaatagc
     1921 tctctttttt aggaccaaat tgtaatattg aaaaccccct ttttcttagt tcttctccta
     1981 tccttatatt tacatcaaaa gtatctaacc ctccataaag accacaaata aatattattg
     2041 gtgtgtttat ctgtgatata ccctctatat ctcttatttc atatttttct aaattccatt
     2101 cattaatctt tttgaattca taatgagata tatacctaga tattttatta agatcatcac
     2161 gtgtatataa tatcttttta ccttctttta gagctacttt taatttagga attataaatt
     2221 tttctaagtc tagattttct tttgaatcaa caaaccatac caaagaattc tttttaagtt
     2281 cactttgaaa atcatttgta actttagtaa taatattatt aacattatac tccttattgc
     2341 ataaccccca gccagaagga ctaaccagtg aattaatttc tatatctgaa attttattgc
     2401 aatgatttaa aatagtaaaa aaattataat taaatggata tataagtact ttttccataa
     2461 tcccatcttt cctttcatta gaaaatttaa tattgatatc ctagagattt caaaacgcag
     2521 taatctttca tattattttc tgtagaatgt ctaattctgt cacaatcatg tggttttatt
     2581 tcattttcat tacatttagt atcatcaatc ttagctatac atatatcaca ataactatag
     2641 gcccaacaat cagaacattg ctcttcatat aattttccta tatttaaaag tttttcagcc
     2701 ttttctatat ctactccact atctaaattt ccaatcatac ccattttaga actttcactt
     2761 actctctcac atggaaaaaa ttctccatct gtagttacaa atattctaaa agcccctggt
     2821 atacatggtc caccatgatg ccacacatct ggtaactcta atctaccttc aatatttttc
     2881 tcatatctaa aatcatctaa taaggctctt tctattcttg ataaaggtga catactctca
     2941 tcttttataa attttaattt gttgagtaaa gtcaagaatc tatcataact cttacctaat
     3001 atatattcat ctgatgaaac tatttcttta tcagaatagt tattattaag agtggaagct
     3061 aaaaagtttg aatcttttaa agtctcattt ttagatataa aatcatctat agaaatgtaa
     3121 tcattttct
//
LOCUS       DACTGW010000313         3041 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.316, whole genome shotgun sequence.
ACCESSION   DACTGW010000313 DACTGW010000000
VERSION     DACTGW010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.316"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            18..386
                     /locus_tag="I9573_003488"
     CDS             18..386
                     /locus_tag="I9573_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAT4798073.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            389..760
                     /locus_tag="I9573_003489"
     CDS             389..760
                     /locus_tag="I9573_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAT4798074.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(904..1359)
                     /locus_tag="I9573_003490"
     CDS             complement(904..1359)
                     /locus_tag="I9573_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798075.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1398..2663)
                     /locus_tag="I9573_003491"
     CDS             complement(1398..2663)
                     /locus_tag="I9573_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HAT4798076.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1136 a          416 c          411 g         1078 t
ORIGIN      
        1 atcaaaacga ggtacttatg gaaaacttgg ttaaaatatt taaagcattg agtgatgaaa
       61 ctagacttaa catacttata ttagtatcca agagaaatat atgtcaaaag gggatttcta
      121 aatatctagg gatttcagat tctgctgttt cacaacatat aaaaatatta aaagatgttg
      181 ggataataac aggatataaa gaaggatact atgttttata tcatattaat aaagaatcat
      241 ttaatatatg tgtagatttt ataaattcta tgttaagtaa ttctagtgaa agctttatag
      301 atgtatttga tgtaaataca atacatctag gatgcagtaa agaatgtaaa tctataaaaa
      361 aatgttgtaa gagaagggag aaataataat gaaagtatgt ataccagtag aggagaataa
      421 aggattggat agtaaaccat atggacattt tggttctgct ccaatttttg ttgtgtgtga
      481 tttagaaagt ggagaagtta aatctttaga taatggagat ttagatcatg aacatggaaa
      541 atgtcaacca ttaaaagcat tgtcagggac agctgtagat gcagtagttg ttggaggaat
      601 aggacaagga gctataatta aattaaatgg aatgggaata aaagtttaca gagcagaagg
      661 tgatactata agtgctaatt tagacttact taaaaatgga aaattagtag agtttccatc
      721 agaccatact tgctcacatg acggatgtgg acatcattaa aaaaaacagc tgatgaaata
      781 tatacttcat cagctatttt ttcttttaat aaatacgatt ttattctttt aaaaccaaaa
      841 tttagatttt tatgtttttt gtagtatcct taataatatt tcaagtttac aagtttacta
      901 cttttagatt ttttttgtat gtttttctat atagtcaata tcatttaata tgtcattgag
      961 ttctttatta agtgttttta aatcatcacc atctacattg tagtttaaaa atagttcctc
     1021 tacattatca atgatagaat tgacttttct agtaacttca acaaatgtct taaagttact
     1081 actattttta gcatttagaa atttttttaa atcctttgat ataaatgata ctatttcata
     1141 ttcatcatta aatagacctc taccaatagg atatggttca attctctgta aaaaatattg
     1201 gtctttagca ttaatattat aagtataaga tatttcccaa ccgtctccta aatcaaactt
     1261 gataaaatat aatgtactac ttacttttga tattcttgtg gcaccaagct gtcttaaaag
     1321 aacttcaatc gaatcacctt gaacagtatt aatattcata tatacacatc ccctttagta
     1381 ttattatact atatttttta aaattttata attaaataaa cagtagatat aataatagat
     1441 ataatcataa gtggaaaacc tattttgaaa tactccttaa aagagatagg atgaccgtgt
     1501 ttattaccta tacctgctag tacaacatta gcagatgctc ctattagtgt tccatttcct
     1561 ccaagacaag ctccaagaga tgttgcccac catagtggca ttacatcaat accttcagct
     1621 tgcatagtca aaataagagg aattagtgtt gcaacaaatg gaatattatc taagaaagaa
     1681 gagactatag ctgataacca taatatcaac aacattgtaa atacaagatg tccttctgta
     1741 agtccaatca atgcttgagc caatttgttt attatcccaa cttctactag tccaccaact
     1801 actacaaata atcccataaa gaataaaatt gtggaccatt caatacctgc cataatttca
     1861 tctacatctt gttttcctat taaaagcatt atagatgctc ctgtcagtgc tacaacagaa
     1921 gaatctatgt ggattgtact gtgaaacata aaaccaaaaa gtatgaatgc tattaatata
     1981 aggcttttta taagtagagg tttatcttta acagattttt tttcatctag ttttaatatt
     2041 gcatttttag ctctttcatt cacaacaagt tcttttccat aaataaatct aaagcatatt
     2101 atagttatag caagtattac tataacagca ggacctaaat ttattacaaa atccataaaa
     2161 ctaagattgg cagcactacc aatcataata tttggagggt ctcctataag agtagctgtt
     2221 cctcctatat ttgaagctaa aatctgtgtt attaaaaatg gaactggatt aagtcctaat
     2281 atttgtgtaa taactatagt cattggtcca ataagtaaga cggttgtaac attatctaat
     2341 acagctgata aaatagctgt tataatagca aaacaaatca ttatcttcca tgggtcacct
     2401 ttagctttct ttgcagtcca gatagctata tattcaaata accctgagtt tttgacaaca
     2461 gctacaaata gcatcatacc aactaataca ccaatagtat tgaaatctat atggcttaat
     2521 ccttcatcaa gtgtaagtat attgaatatt atcattaata gtgcaccacc aacagctgct
     2581 acggatctgt ttaccttttc tgtaataata gctgccataa ctattaaaaa tatacatata
     2641 gctattattt gttgaaattc cataatataa gtacctccat ttgtattatt ttgtgcctaa
     2701 tgctagtact atatatagta ttactttttt attgtttttt caatggatgt atgaaagtat
     2761 attatttaac aaagtttttg agaaattgac agaatttaaa gaaaattttt gttatttaaa
     2821 taaattttaa aattattcca ttgataataa tattaaacga caaacgaaaa aatataccct
     2881 ttaaactaga atttattgta tccaataaca attataaaat attaaatata tctatattat
     2941 aaaaataata aaaaaaggtt tgatttgaat ataaaactgc aatatattaa aaaaattatt
     3001 cattttaaac cttgtaattt gattttataa atattataat t
//
LOCUS       DACTGW010000314         2953 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.317, whole genome shotgun sequence.
ACCESSION   DACTGW010000314 DACTGW010000000
VERSION     DACTGW010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.317"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            42..1886
                     /locus_tag="I9573_003492"
     CDS             42..1886
                     /locus_tag="I9573_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAT4798077.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKRKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASNAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDIVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            2208..2792
                     /locus_tag="I9573_003493"
     CDS             2208..2792
                     /locus_tag="I9573_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAT4798078.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKDKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNVKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         1165 a          286 c          446 g         1056 t
ORIGIN      
        1 catatgataa aattagaaca aacagataca aggagataaa catgcaaaag agaattttaa
       61 gaaattttat atttacgggt aaaaatattt taagtaaact tgtttttata ataaaatata
      121 attttagtac attaatttta tttgaattat ttcataaagg attagcctta ttttttatat
      181 ggccatctat tagatatata tttgatattt taataaaaag tttacatata gtctacctaa
      241 atatggataa ttttataaaa gttttgacca atcccatgtc aatagttctg ataatactat
      301 cagttataat actagctttt tatgcatttt ttgaatttac ctctgtaatt atttgtttta
      361 ataaatcgat aaaatatgaa aaaatagggt tatttgaact ctttaaaata tcatttaaga
      421 actctataaa attgttatat cctaaaaata tactattatt tatatttgtg atattaataa
      481 ttccattagt aaatacagtt ttaatttcag ggtttatagg aaaaatacaa ataccagagt
      541 acatattaga ttatataaag tctgatataa ttttaaatat aatctatatg atttttcttt
      601 taataatcta tatttcggtt attagatgga tttttagcat tcatgagata actttaaata
      661 cagaaaattt taaaagagca agaaaagaaa gtgttaatct tataaagaga aagattataa
      721 gaacaattat ttattcatta atattatctt tagtagtagc tattattgga tatgcgattt
      781 atcatagtgg aattatattt ataggcatat ggactaaata ttgtactgat atggaatttt
      841 tgaagaatgt atttataaat agatgtattg catttgaaga atattttaga ttgattttat
      901 caatattcat ttttatattg gacattggat ttatctcagc aacttattat caatataaag
      961 gagtaaatat ttctaaaaat aatattaaaa aagagaaaaa atctatatca aaaattgttt
     1021 ttaatttagt attaatatta ttgatttctt atatagagtc agttgcattt tcttcacata
     1081 taaatggcat gtttaatacg tcattttcat tttatacaac agcaattgct catagagcag
     1141 gagctgaaat ggctccagag aatactttgg cagcaataga agaagcttca aatgctaaag
     1201 ctgattatgc agaaatagat gttcaagaaa ctaaagatgg agaacttatt gtacttcatg
     1261 attcaaactt taagagaact acaggagtag ataaaaatgt ttgggatgta aattataatg
     1321 aagtaaagac atatgatgcg ggaagctttt attattatgg aaaaggttat tatgatgaaa
     1381 agtttgttgg ggaaaagata ccaacactca aagaaatgat aaaatattct aaaggtaagg
     1441 tgaaattatt aatagaaata aaacttaatg gacatgaaaa gggagacata gtaaaaaaag
     1501 ttgttcagtt aattaaagaa aataattttg aaaatcaatg tatagttgca tctatggata
     1561 aaactatatt acaaaaagta aaaaaactgg atgagaattt actgacatgc tattttatgg
     1621 caattgcata tggtgatttt tcaaagttaa attatgttga tatatatgga attgaatcaa
     1681 cttttgttaa taaaacagta gtagaaaaga ttcataaaat gaacaaaaaa atcttcgttt
     1741 ggactgttaa tagtgacagt ttaattgaga aaatgcttga cctaaatgta gatagtatta
     1801 ttacagataa tccatacctt atagagagtg caatttattg gaagaaaaat ggatttatta
     1861 gtcaagtgtc agattattta ttttaaatat tctataattg tattatccag taaaagagat
     1921 attattaaaa tcaataatat ctctttttaa cataaataag gttgttaaat taaggttatt
     1981 cttataaaaa ttgtgcttat cagctataaa caaggaataa aagtgaaaaa attgctctga
     2041 gattattaca gttaaaagag ttttaagatg acagagagat agattgtcaa ctgtatattt
     2101 aataaatctt gttatttatt aaattttata taagtaaaat atataaatta ataaatctta
     2161 tgctagaata tatatgaaaa acaatataaa tttggaggat taaaaacatg acagagcgag
     2221 aaaaaatgtt ggcaggagaa ctttacgatt gtggtgatat tgagttgtta actcaatggc
     2281 ataaagcaaa agacctagta agagattata accaaacaga ttcctcggat ttggaaaaaa
     2341 aagaaaaaat attaaatgaa ttacttggtg gtaaggataa aaacttatgg attactgcac
     2401 cgtattatgt agattatgga aataatattt attttggaaa taattgtgaa gtcaatatga
     2461 attgtacgtt tttagatgat aataaaatta tcattgggga taatgcacta atcgcaccaa
     2521 acgtacaaat atatactgca tttcatccaa ctaatgctgg tgatagattt ggtaatgtca
     2581 aagaagatgg ttcatttgaa ttttgtaaaa ctcaaacagc tccagttact attggagata
     2641 atgtttggat tggcgggggt gctattatca tgccaggtgt tactattgga gataatgtag
     2701 ttataggtgc aggaagtatt gttacaaaag atattccaag taatatgatt gcatacggaa
     2761 atccatgccg tattattcga gaaaacaaat aaagttaaat tttcttgcaa aatctcaaaa
     2821 aacacctttc ttactttata catagggtat tttccaatac tttcacatcc atattcattt
     2881 gttaaatttt tgtggaatgt agtttttatc acttctatct tacatttcca ccatttttta
     2941 taaaagtcat tcc
//
LOCUS       DACTGW010000315         2937 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.318, whole genome shotgun sequence.
ACCESSION   DACTGW010000315 DACTGW010000000
VERSION     DACTGW010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.318"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            190..528
                     /locus_tag="I9573_003494"
     CDS             190..528
                     /locus_tag="I9573_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4798079.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            550..867
                     /locus_tag="I9573_003495"
     CDS             550..867
                     /locus_tag="I9573_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4798080.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1017..1694
                     /locus_tag="I9573_003496"
     CDS             1017..1694
                     /locus_tag="I9573_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798081.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2022..2789)
                     /locus_tag="I9573_003497"
     CDS             complement(2022..2789)
                     /locus_tag="I9573_003497"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAT4798082.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKVAVMPQNERDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
BASE COUNT         1185 a          379 c          348 g         1025 t
ORIGIN      
        1 gaaaatattt ctctaattaa aaatatgatt tatccatatt tgtttattgt agatacagac
       61 tatacaaaaa taaatagcac ttatttcagt ataaaaacat atatagtcta tatttagact
      121 aaaaaagtat aaatagtcta tataaaacaa cttaaactat gaatataatc taaagtggag
      181 ggataataat tggcaaatat aggaaaaata ataggagata gaataaaagt ctatagaaca
      241 aaattgggat atagtcaaga gtttctagca gaaaaggcag gtttacatcc aacatatatt
      301 ggtcagattg aaagagctga gacaaataca tctatcaata ttataatgaa aattgctact
      361 gctttagaca taccacttga attgttattt gcaaatatta ttacaacaga aggcgttgat
      421 aactctgtac ctttagaatg ttatgaaatg attaataaat taactgaaaa agaacaaatt
      481 gcaatgctag agttaataaa gtgcatcatt aaatacaaag aaatataagt gagggaaata
      541 gtaacaataa tgaattatat aggtaaaaga cttaaagaag aacgaaaaaa agcaaattta
      601 acttcaaaag aatttgcaaa tatggttgga gtatcgcctt ggtatataac tcaaatcgaa
      661 agtggtaaaa agaatccaag tcttaaaaca ttcataaaat ttgtaaatat attaaatatt
      721 tctgcagatg ttcttataaa agatattact tccactggaa aaacatattt agaaaatgat
      781 ataaatgaag agttaaaaga cttaaattca agagaactaa atctgatatc acagattata
      841 gacatcataa aaaagaataa aatataattc tttatatttt atataaaaaa aaaggttatt
      901 tgaaataaat aacctttttt tgtctttttg tgttaacatt gtcttatagt gctaatgagt
      961 actattagac catgttctat ggccttattt taatataaaa cgattggaga taggctatga
     1021 ttgtgattga aggtagtgat aaatttaaga ttgcaaaaga atatattgat gtagaatata
     1081 ctctttttag caaagtaact tttaggtatg aaaagttgaa atttaaagat aatgctgaat
     1141 tggaaaaaat taagatgttt aaatataaaa atggctacat ccctaataaa ttaaaccttt
     1201 cttttggata tggattctct tcttataaaa agcaaataat tagagaaact gtagatactt
     1261 taagattgac agaaattttt tcaagcgaga acatagaaga tattaaattt ataaaagatg
     1321 gtacaaaaaa attagaaatt agcatagaga aagttgtgaa atttaaacgt cgaaaaaaaa
     1381 gaaattatgt ttgttgctat tgccctgata tgtatagaga tataaaactc gacaaagaat
     1441 ctatcaataa gatatacaac agaaaaataa aaatagaaag agaagttaat atttttgaag
     1501 atgaggatgt tataataaac aagaaagtat tgaagtttcc aaagtcttgg acaaagaata
     1561 tgcaaaaata ttggttaagt gaaaataagt atcccataca ttctactgta attgatgatg
     1621 atagatataa atgttgtaat gtacaatata caaaaaatag agtgataata ttatattaca
     1681 tatataacca ttaatataca taattacaaa aaaataatga caaatataca taatacaaat
     1741 atatcttcta ctttagataa aacagttatt atgaaaactt actatctata aataatagaa
     1801 atgctagtat accatctata atctaatgtc tagtaggttt taatggcata ttttaaatat
     1861 gccatagttg atttataaga ttttctatat cactttcaaa atacacacta aatagttgct
     1921 tttcagatat aattgcccaa tttaaatttt actatctgaa aagtttttta tatttaattt
     1981 atatcttcaa aaataccgtc taatatttct atataattaa tttaattaat actcttatgt
     2041 aatagattaa gtactgaatt taactcttta aatgatattt gccctggtgc tgaaacactt
     2101 tttgcagctc caaaagtcaa tgcagaacca aatatttcac cacacaatct acttataaca
     2161 cccattcctg acattgacat tgttattatt ggtctatctg cataaatttt aaacatttca
     2221 tttgtagctt caagtagcac taacacatct ctttcatttt gcggcataac agctactttg
     2281 ggtaaatctg cgcccaattc ctgcattcta cataaacgag atacaatctc ttcttttttt
     2341 ggagttttat taaaatcatg attagatata attactttca cttctttttt atgagcaaaa
     2401 ttaactactt catctatgac ctcatctccc ataaataact ctacatctat taaatctact
     2461 aaacctgtat tagaaatttc tttattcaaa gtagtataat aatctcttga tattaatttt
     2521 tctccccctt ctactacact tctaaatgtg aataataaag ggatatcatg tatataactt
     2581 cttaattcat agagaacttc cttaacttct ttaatatttt ctacattctc aaaaaaatca
     2641 actcgccact ctataatatc caaacatgca tcttttaact ctttagcctc ttttataata
     2701 tcttttttat ttttcccaat aattggtaca caaattttag gtctaccttc tccaatagta
     2761 atatttttta cttgaacttt tcttttcata ttctcctcca taatcagaaa ttctatagga
     2821 ctttaaattc tattttctgc ataatttaat tatacattaa aaatactata cattataaaa
     2881 atactaattt aaaaataatt tttattctaa ttcttaaaac aatcccacat attttag
//
LOCUS       DACTGW010000316         2932 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.319, whole genome shotgun sequence.
ACCESSION   DACTGW010000316 DACTGW010000000
VERSION     DACTGW010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.319"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..696)
                     /locus_tag="I9573_003498"
     CDS             complement(<1..696)
                     /locus_tag="I9573_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAT4798083.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESY"
     gene            complement(974..2359)
                     /locus_tag="I9573_003499"
     CDS             complement(974..2359)
                     /locus_tag="I9573_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAT4798084.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(2467..2871)
                     /locus_tag="I9573_003500"
     CDS             complement(2467..2871)
                     /locus_tag="I9573_003500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798085.1"
                     /translation="MFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIAK
                     INYNKNGETYGTHIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAV
                     LYTRKRNFNIFKKTIPLYDKEGIKIIGRFEIN"
BASE COUNT         1005 a          428 c          370 g         1129 t
ORIGIN      
        1 ataagattcc tcctttttac agtaagattc ttctataaag aaaccaatta aagatgctaa
       61 aattgatgca actattatta ctaaattgct tttgaataat gcatataaaa taacagaaat
      121 aatgattgat gatataaata ctacaatata aatcttattt tttatttgag aaactattaa
      181 agctataaac atagaagtca atatgaagcc agaaatagaa ctgtctatag atatactttc
      241 tcctaaaaat gctccaataa aattagctaa aactgatgaa attaagcaga agaaattaag
      301 ataaatagca tttctatcag accaatttcc agcagtaaat ttttcataat taactgcaaa
      361 agtttcatct gttatagtat gacaaaatgg caatataaat tttggacttt tattttttat
      421 gtacatgttc aaattagaac tcattagaaa atgtcttaag ttaactataa atgtagtcaa
      481 aacaatagat gttacacttg caccagtaga aatcattgaa gctgcaatga attgagaact
      541 tcctccaaat actaaagtta ccattagtgc tatttgaaaa ggtgaaagac cagatttttg
      601 taatagtatt cctcctacta tacctatagg tatataacct aagaatatag gaattgaagc
      661 aataaagctt tcttttaatt gttgagttct ttccacaata aatcactcct tttgtattaa
      721 atgtattaaa gtattagtaa aacagaaaaa tagattaatt gtataattgc aattataatt
      781 aaaaataaat ttggattaat atataaaata ctatacaaca aataaataat cttaaacggt
      841 tattaaattt tatctatttg ttattatgat gtaataatac atattaatct ctataagtta
      901 attactatct ggtgtaattt tattttaaca acaaaaaaaa atacacacaa ttaaaatatt
      961 gtgtgcaata caattatttt ttctcttcta atagtctttt taatacgctc agaccagctt
     1021 taagttcttc tgtgctttct gtagcacata cagctattct tattgcagaa attggttttt
     1081 cttttccaac agcaaaacgt tcagaagcat agacctgtac accttcttta agtgcttgta
     1141 tctcaaattt ttctgccatt attccatcag gtagaatcaa ccatctaaat atacattctt
     1201 catttcccaa aagattatat ccagataaat attgattaat aatcttgttt cttcttcttg
     1261 cagatttacg gcgtgcatta attaatttat cggcttcacc tgatgtaatc atgcgataag
     1321 caatttctgt taaaaaataa gattgagata gattaatgtt gtataatgcg cttgataatg
     1381 cctctcgata ctgtttaggt gtagagatat atgctagccg taaactaggt gcaataattt
     1441 ttgataaact cgtaatataa attgtttgat tgggcagata ggaagccact ggatttaaat
     1501 gtgtttcatt aagtaaacta tgaatagcat cctcaataac aattaaattg tatttagatg
     1561 caatatttgc aatcattttt cgtccatttt gtgacattat atgtgtagtt ggattttgat
     1621 aatctggtat tatgtataga cctttaatat tttcattttt acaagcatat aatagtcctt
     1681 cttcactaat ctcattatct tcttgtttaa ttggaattag ttgaatccca agcattttag
     1741 ctgctgtctt tattccagga taagttaatg ggtctacacc tattctatct ccatgttgaa
     1801 acaaaccagc taaaatagca acaattgcat tttgtccacc acttgcagga agtaagctat
     1861 ctgatgtagt ttcaagccca gctttataaa ttaatttggc aatagcttct ttttgccatt
     1921 tagcaccatc tctttgtcca tattggaata tagttttaaa atctatttcc ttaattatat
     1981 gtttaaacaa agtattcatt tcttctagtg tgaaatctgg attcattgtg cccatttcaa
     2041 taaatgtaat tttattatta ctggacatca agaatagatt tgaacgcgta tcataagata
     2101 caaaagttcc acttcctgta actccactta ttagaccttt tttttcacaa attttaaaag
     2161 ctctagagat agtactcaca ttaacatcta agaaatctgc caattcacgt tggggtggta
     2221 atttagtacc tggtaataaa aatccattgt tgatatcttg ttctagttgt tctgcaagcg
     2281 ccttatataa tatttgccct tttgaaggtc tctttggttt ccagttcata ggataatttt
     2341 caaaagaatt aattggcata agttttcacc tcacattatt aatttgattt tttctttatc
     2401 taaaacaata aattctaaag atacaaataa aaactgatag gaaataattc tatcagtttt
     2461 tgtcttttaa ttgatttcaa atcttcctat aatttttata ccttctttat cataaagagg
     2521 gattgttttt ttaaatatat taaaatttct ttttcttgta tataaaacag cttcttctgg
     2581 tgtctttggt tgattatcac aatcgtacaa atctcttttt tttacgtagc cgactttttt
     2641 acctctacct attgtttgta taagttctgg ttcaatggaa tttccatttg agtctattat
     2701 atgtgtacca taggtttctc catttttatt ataattaatc ttagcaatag tatattctac
     2761 atcttcattt tgtttaaaat tggagttgat agaaaatatt gaccctatta ttataaaggt
     2821 taataaaaac ttaagaccta aattaatttt ctttttatct tgattaaaca tagaatttaa
     2881 cctctttttt aaaataatat tttttttatt tagaaactga gatacgaatg at
//
LOCUS       DACTGW010000317         2890 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.321, whole genome shotgun sequence.
ACCESSION   DACTGW010000317 DACTGW010000000
VERSION     DACTGW010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.321"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            125..1201
                     /locus_tag="I9573_003501"
     CDS             125..1201
                     /locus_tag="I9573_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAT4798086.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1304..2146)
                     /locus_tag="I9573_003502"
     CDS             complement(1304..2146)
                     /locus_tag="I9573_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAT4798087.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2330..>2890
                     /locus_tag="I9573_003503"
     CDS             2330..>2890
                     /locus_tag="I9573_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT4798088.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVN"
BASE COUNT         1033 a          404 c          415 g         1038 t
ORIGIN      
        1 attaaactta atataaaaaa tatataacaa acttttttta tatttctaga agaaaagtat
       61 actctttttt gtttaataat gctaaaattg atttgtattg aattttgatt aggaggaaat
      121 tattatgaaa gctcaaagat taaatgctgt acttgaacaa atgaaaaaag atgatatttc
      181 tcaaatgtta gtaagtgacc ctacttctat attttatctt acaggagtac taatccatcc
      241 aggtgaaaga ttattagcat tatacttaaa tttaaatggc aataataaat tatttataaa
      301 tgaattattc ccagtttcag aagatttagg tgttgaaatg gtttggttta atgatactca
      361 aaatccagta gaaataataa ctgaacatat agacaaaaat gctactatgg gtgttgataa
      421 aaactggcca gctagattct tattaaactt aatagaatta ggtggaggaa gtaagtttgt
      481 aaattcatct tatataatag atactttaag aatgtgtaaa gatgaagaag aaaaagagtt
      541 aatgagaata gcttcaaaat taaatgacaa ggctatggaa caacttaaag ctactgtttc
      601 tggtgaactt acagagaaac aacttgttgg taaattatct aaaatatatg aagacctagg
      661 tactgatgga ttttcatttg accctattat aggttttgga cctaatggag ctaatccaca
      721 tggtgaacct ggaaatgcac ttgtgaaacc aggagatgca ataatattag atataggttg
      781 tataaaagat aactattgtg ctgatatgac aagaactgta ttctataaag aaatacctga
      841 aaaaggtaga gaaatatttg aaatagttct tgaagctaat aagagagctg aagctattgt
      901 aaaaccaggt gttagattct gtgatataga tgctgctgct cgtgactaca taactgaaaa
      961 gggatatgga cagtacttca ctcatagaac tggtcactct ataggacttg aagttcatga
     1021 taaaggagat gtatcttcta taaatacaga tactgtacaa ccaggaatga tattctctat
     1081 agagccaggt atatatttac caggtgagtt cggagttaga atagaagatt tagtacttgt
     1141 tactgaagat ggttgtgaga tattaaacaa acatgacaaa gaaatatgtg tagttggtta
     1201 attctaaaat caatttgata aaattaaaaa gccatataaa aatgttttaa acatttttat
     1261 atggctttaa tttctcaatc tatgatttat ttcaatccat tatttatatt gcagttatta
     1321 agattgcacc ctttgaagta tatgtcccca tcactgaacc aatttcacag attacaacat
     1381 ctttgtattt tacatccttt tccataactt cccttacctt ttgagccttt tctgggcaat
     1441 tagcatgtcc tataaataaa accttttctt ctttatcttt aatttttgac tctactaaag
     1501 taatcagctt tttcaaactg tttccttcac ctcttgcctt atctatagga gttacaacgc
     1561 catctgcaac ttgtattatc gccttaaaat tcaaggtatt tataattttt cctgctattg
     1621 gatttattct accaccctta atagcatttt ctaaagtttc aagtgttcca aaaaatggag
     1681 gattatcttt atatttattt attattgaaa caatctcttc aatttctttt ccttctttta
     1741 ttaatattgc tgcttttaac actaattgtc ctattccaat tgacccacta agtgaatcta
     1801 taacctcaac ttttttgtta tttccttcct tttcaaacat atcttttgct aaaagtgcac
     1861 tattataagt cccagataat cctgaactta tattaatcac caatacactt tcttcctgac
     1921 aatggtatgc ttctaaaaat ttatcaggag aaggacatga tgtttttggt agtactttac
     1981 tctcactcat ttttctatag aaagtttcat tatctatttc ctttccagat atataacttt
     2041 cttctccaaa agatacattt aaaccaacaa cttctatgtt gtatttttct attatatcat
     2101 caggtaagtc acatgcactg tccactatca attttatttt atccatatta acctcactat
     2161 aagatattat tttatttatt cagtcggtcg tacgaccgat ttaattatta ttttatatat
     2221 tgaatgaaaa aaagtcaata gatatagatt ctcgctttat tgacaataaa tattcataag
     2281 cttataatgt tattaatttg ttataaatat acatggaaag gtaggtttta tgaaaacaaa
     2341 taatatgaaa caggctattt tatcatctgc aaccaattta atagccaaaa atggagttca
     2401 aaatactagc cttgctgata ttgcaaaaga tgtaaatata agtaaaggta ctttgtatta
     2461 tcactacgca tctaaagatg atattatata tgatattgcg gatactcatt tagaagttat
     2521 aactagtgct atactaaact gtgtaaaaaa tgtcaaatca aaaaattctc aaatagaaat
     2581 ggttaactta atcttagaga aaatatctac tatagaaagt cgtggtaggg tacatatgta
     2641 tcttatttgt gaggcaatta cttcaaataa tgatttaaag gaaagaataa gattaaaata
     2701 tattgaatgg agaactactc ttaaatctga aattgttgaa tcactagaaa aatataatag
     2761 tagcgaaaat gaacaggatg ctgaatcatt ttctttttta ctcatgtcaa tagttgatgg
     2821 tttagttgtc caaagtttat taaaaacaga aaaaatacct tatgaaaata tagcttcttt
     2881 tttagttaat
//
LOCUS       DACTGW010000318         2868 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.322, whole genome shotgun sequence.
ACCESSION   DACTGW010000318 DACTGW010000000
VERSION     DACTGW010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.322"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            318..1589
                     /locus_tag="I9573_003504"
     CDS             318..1589
                     /locus_tag="I9573_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAT4798089.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSIADTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPM
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     gene            complement(2228..>2868)
                     /locus_tag="I9573_003505"
     CDS             complement(2228..>2868)
                     /locus_tag="I9573_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HAT4798090.1"
                     /translation="IFDVNNYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNI
                     IIWCANNPKDLCGVYYVVSKFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFL
                     DKTLEIGFNEKLEFKNKWDVLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQP
                     LIEVVANCMFEDEMRVKDWFILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT         1064 a          368 c          347 g         1089 t
ORIGIN      
        1 atactaatat aaaaataact atgaatagat attttcttgt tttaaacgat atggataaaa
       61 aagcttttga aaatataatc aaagttctct ttaaataaca ttccatttta tgtaagtaag
      121 cattatattt ttcttaaatg tcacttaagt gtcagttata tataataata gtaaacaact
      181 gtatttcact aatatttgct attatctaat agtatttata gtttagattt gatataatac
      241 atgtggtaaa attttgattt gtaaaacaaa attttaccta catatattaa aacaaattta
      301 aaactaagga gacctatatg ttaaaagtat taaaaggaaa tataattttt acaaaaactt
      361 cagaaacttt cacagtattt gaaaatagtt atattatact ggaaaaaggt aaagtaaagg
      421 gaatatttaa agagatacct aaggaataca aaaacctaaa agtagtagat tacactgata
      481 agcttataat acctggtatg aatgacctcc atgcccatgc ctctcaattt aaaaatcttg
      541 gaatggctat ggataaagag cttttgccat ggcttgaaac ttatacattt ccagaagagt
      601 caaattataa agatatagaa tatgcaacaa aaatgtataa aaaatttgta aaagaacttt
      661 ggaaatctgg aactacaaga atagcagtgt ttgcaacagt acataaagaa gcctctatga
      721 aacttatgga tatattcaaa gaagttggtc ttggagcata cgttggtaaa gtaaatatga
      781 atatgaattg ccctgatact ttattagaaa acactcagga ttctattgct gatacagaag
      841 aaattataaa taagtatagt gatacagatt ctattgtaaa accaataata acacctagat
      901 ttataccaag ctgtacaagt gaacttttaa aaggtttagg agatttatgt ttaaaatata
      961 atattcctat gcaatcacac ttatctgaaa attatgatga gattgaatgg gttagaagtc
     1021 tagaacctga atctaaattc tatggagatg catataatag atataattta tttggtcaaa
     1081 ctcctacttt aatggctcat tgtgttcatt gttctcagga tgaaatagat ttaatgagag
     1141 aaaacaatgt tatggcagtt cactgtccag cttctaactt taatgttggt agtggtgcta
     1201 tgcctatcag aaaattaatc gataataata ttagattagc acttggatca gatataagcg
     1261 gtggacatac attgtcaata tttaaagcaa tggtatctgc aatacaatta tctaaattat
     1321 attgggttaa ttctggtaag aagtataatt tcttatcttt atctgaggct ttttatattg
     1381 caaccaaaag tggtggaagt ttctttggaa aagttggtag ctttgaagaa ggttatgatt
     1441 ttgatgcatt gattattgat gattcaaatt taaatcatga caattattct atacttgaaa
     1501 gacttgaaag atttatctat gttggtgatg atagaaatat cattcataga tatgtatgtg
     1561 gaaaactaat tgaagaacct aatatataat tataataaaa attttaatta tatattatgg
     1621 ttaatttatg caactataat taataaaaga gtatatttta aatctaaaag ctataggttg
     1681 aaacatactc tttttatttt gtttatttat agctttagtt attaatcata tataaatcta
     1741 caaaacgtat tcctagtaac ttatattttt tgatatctac aatcataaga ctatattatt
     1801 ggattcatac ctcaaaaata ttgccttctg aacattatta tattcaaagt tatgttaatt
     1861 tacaatgata tatctaacat gtattgatta aaaaaaatta aaacttgctt ttcatctcct
     1921 atatctaaag aagtggcatt tagaataaac tattgtaata ttaaagttga aattttctag
     1981 tttatttact ctatagcacc atattttaat actattaagt aaaaatgtag ttaaaatata
     2041 tactaatatc cctgaaataa gtatttcttt tttatttagc acacttattc gtaaggtatt
     2101 agttattata ttttcaaatt atagaataaa tattatactt attgctaatt ataatattaa
     2161 tactgttaag atatttttct tcatagttat acccattatt taatacaatt tattataaat
     2221 tcaattttta aacttttctt atctccttat cagaataaaa atccccttta cttcctttta
     2281 tttttactat ttttaaactt gccatcttct ctaatctcca aagtataaac caatctttta
     2341 cacgcatttc atcttcaaac atacagtttg caaccacttc aattaaaggt tgccatttgt
     2401 cactgatatt tcttaatata tatttatcaa aataagtttc ttcaacactt ttaatttcgt
     2461 tacctactaa tgctctaaaa atagaatttt ctttaactaa cacatcccat ttatttttaa
     2521 attctagttt ttcattaaag ccaatttcta gtgtcttatc taaaaagaat ggaaaatctt
     2581 ctggagacat ttcactggta aatgaatatc taattagagc atcttctgtc tttttttcat
     2641 ctactaatgc aatctttata tttttatctt gaaacttact cactacataa tatactccac
     2701 acaaatcttt tgggttattt gcacaccata ttattatatt tctacatttt gataatttcc
     2761 tatcttctaa caaatctggc ttgaataata gtttcggatt attttcaaaa acatttctaa
     2821 tcttaaaaat attttctctt aattcgtagt tatttacatc aaaaatat
//
LOCUS       DACTGW010000319         2829 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.323, whole genome shotgun sequence.
ACCESSION   DACTGW010000319 DACTGW010000000
VERSION     DACTGW010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.323"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            293..796
                     /locus_tag="I9573_003506"
     CDS             293..796
                     /locus_tag="I9573_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HAT4798091.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            843..1802
                     /gene="moaA"
                     /locus_tag="I9573_003507"
     CDS             843..1802
                     /gene="moaA"
                     /locus_tag="I9573_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAT4798092.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            1806..2276
                     /gene="moaC"
                     /locus_tag="I9573_003508"
     CDS             1806..2276
                     /gene="moaC"
                     /locus_tag="I9573_003508"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAT4798093.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDYMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            2312..2743
                     /locus_tag="I9573_003509"
     CDS             2312..2743
                     /locus_tag="I9573_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HAT4798094.1"
                     /translation="MAKVVSINISEKKGVIKVPVPCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         1182 a          252 c          545 g          850 t
ORIGIN      
        1 tttgtagaga aaaatatgga caatgttata ataattatag aaattaaaag tacttacatt
       61 cattatgtat gtgacatgta taattagttt tatatttaat acagttttga agtaatatct
      121 tcattttgag aacgttggaa taataaaaaa tgtatgtatt ataggtgata taaataaaaa
      181 ataaatataa tatgttatat tgctgataga aaagagaata aaagaaaata ggttttatta
      241 aaagagattt ttatttatag gacttaatca atgtatagag gggtgagaac atatgtttaa
      301 tgttgcaatt ataacactta gtgataaagg atatgaagga aaaagagaag atataacagg
      361 aaaaaagtta actgaatttg ttgaaaatac aggagcttat aaagttactg agtatgtact
      421 tataaaagat gataaggaaa tgctaaagga aaatattata agactttgta atagtaataa
      481 aatagattta attcttacaa atggaggtac tggtttttca aaaagagata taaccccaga
      541 agcaactaag gaagtgttgg aaaaagaaat accaggtctg agcgaatata tgagaatgaa
      601 atccacagaa attactaaaa aggccatact aagtaggggt gtgagtggaa taagaaataa
      661 ttctataata ataaatttac ctggaagtcc taaaggtgca gttgaaaatc taagttttat
      721 aattgatgtt ttagaccatg gcatagaagt tttaagagga gaagctacag aatgtgccac
      781 taaaaaaagt gaatagtata taatctagat agataaaaaa tagacaaaga ggtagataaa
      841 gtatgattga taagtatgga agaaaaatag actaccttcg tatttcactt acagataagt
      901 gtaatttaag gtgtgtctat tgtatgccac cagatgtaaa atttgataaa aattatataa
      961 atgaaaatct aagctttgaa gactacaagt ttattataaa ggctatggct gaacaaggta
     1021 taacaaaaat tagatttact ggaggagaac ctcttttata tcctaaatta aatgagctta
     1081 taaaatttac aaaagaagaa tgtggaataa ataacatagg aatgactact aatgctattg
     1141 ggctttctga tagaattttt gagcttgaaa aatgtggctt aaataaagtt aatataagtt
     1201 tagattcttt gaaggaatat aagtataaat ccgtaacaag aggaggaaac ttaaaagatg
     1261 tcttaaaatc gattgaatct tgtttaaact taggtataaa agtaaagtta aattgcgttg
     1321 ctataagtgg gtttaatgat gatgagctta aagattttat gtttttaaca gttaaatcac
     1381 caattgatgt aagatttata gagcttatgc cattaggtga ggctaataga gtttataaga
     1441 agggttattt taatgtaaaa gaaatgattg aaaaaatata tggtttatat aaagtcaaaa
     1501 atgatgaaaa aagtacagca gaatattata tgtttaaagg agctaaagga agaataggaa
     1561 ttataactcc attaagctgt tcattttgta acacttgtaa tcgtattaga cttacttcta
     1621 gtggtgcaat aaaattatgt ctacattcaa aagaagaaat agatataaaa ccatttttac
     1681 ataaaccatt gttatttaga gaatctatga aagatataat aaaacaaaaa ccagaaaaac
     1741 atcatttaat agaaaataaa tgtagtgata caaataggcg tatgtatgaa attggaggat
     1801 aagtaatgtt aacacatata aatgaaaaag ggtatgccaa gatggtggac ataagtgaaa
     1861 aagatgatac taaaagaacg gccgtagcta cagctacaat tactcttagt aaggaagcat
     1921 tagaaaaagt aaaaaatggt cagataaaaa aaggagacgt attgtctgta gctcaagttg
     1981 caggtataat gggagctaaa aatacatctt caaacatacc aatgtgccat cagattaaat
     2041 tagttggatg tgatttagaa tttgatgtag atgattatat gtctgctatt aagatatacg
     2101 caacctgtaa atctcttggg aaaactggag tagaaatgga agctttaaat gcatgttctt
     2161 tagcagcact tacaatttat gatatgtgta aagcagttga taaaaaaatg gttataagta
     2221 atatccatct tgtggagaag actggtggaa aatcaggaga ttttaagttc gagtaaatta
     2281 ataatatatt gggttaaaat ttggaggaaa tatggcgaag gtagtgtcaa taaatataag
     2341 tgagaaaaag ggtgtaatta aagttcctgt accatgtgca gagctaaaaa taaatcatgg
     2401 gatagttgga gatgcacatg caggaaactg gcatcgtcag ataagcttac ttggaaaaga
     2461 aagcatagat aaaatgaaaa tgaaaggctt tgaccaatta aaatatggag attttgctga
     2521 aaatgtaact acagaaggga tagaagtttt ttccctgcct gttggtacaa agttaagaat
     2581 agggcaatgt gaagttgaag taactcaaat agggaaaaaa tgtcacaatg gatgtgaaat
     2641 taagaaattg acaggtgact gtgtgatgcc acgagaagga atttttgtaa aggttctaaa
     2701 agaaggaatt ataaatgtga atgattcaat agatgtaatc taatgaagtc actattaaaa
     2761 tataattata agtaaatact attaagattt aaatatatat tttaagagag aattaattta
     2821 aataaaata
//
LOCUS       DACTGW010000320         2798 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.325, whole genome shotgun sequence.
ACCESSION   DACTGW010000320 DACTGW010000000
VERSION     DACTGW010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.325"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            346..906
                     /locus_tag="I9573_003510"
     CDS             346..906
                     /locus_tag="I9573_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin regulator"
                     /protein_id="HAT4798095.1"
                     /translation="MFFGNISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAE
                     PWFKMKDDEKKAIEAENQRKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYK
                     YKKVKFEGKVIQVIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGI
                     SAGTITYESTMGGKITIPGIAVEKIN"
     gene            complement(1241..>2798)
                     /locus_tag="I9573_003511"
     CDS             complement(1241..>2798)
                     /locus_tag="I9573_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase C80"
                     /protein_id="HAT4798096.1"
                     /translation="KFLTLNGKKYYFGSDSKAITGWQTIDGKKYYFNPNNAIAATHLC
                     TINNDKYYFSYDGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHN
                     NNIEGQAIVYQNKFLTLNGKKYYFDNDSKAVTGWQTIDSKKYYFNLNTAVAVTGWQTI
                     DGEKYYFNLNTAEAATGWQTIDGKRYYFNTNTYIASTGYTIINGKHFYFNTDGIMQIG
                     VFKGPDGFEYFAPANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGK
                     KYYFNTNTAVAVTGWQTINGKKYYFNTNTYIASTGYTIISGKHFYFNTDGIMQIGVFK
                     GPDGFEYFAPANTDANNIEGQAIRYQNRFLYLHDNIYYFGNDSKAATGWATIDGNRYY
                     FEPNTAMGANGYKTIDNKNFYFRNGLPQIGVFKGPNGFEYFAPANTDANNIDGQAIRY
                     QNRFLHLLGKIYYFGNNSKAVTGWQTINSKVYYFMPDTAMAAAGGLFEIDGVIYFFGV
                     DGVKAPGIYG"
BASE COUNT         1026 a          392 c          399 g          981 t
ORIGIN      
        1 atgtttcatt taatatataa aattcacctt caagaaaatt atattataat ctgacatttt
       61 tacctcattt ttcaaaatat attgaatctt cttgatttat tttgtaaaat tatgcttagg
      121 ggaaatatat tttaggaaaa tatgaatata taatttttag tcaactagtt attttaagtt
      181 tttaaatttt aaaataaaat atatctaata aaagggagat tgtattatgt tttctaaaaa
      241 aaatgagggt aacgaattta gtaatgaaag aaaaggaagc tctaagaaaa taattaaatt
      301 ctttaagagc acaaaggata ttgctctact ggcatttatt ttggtgtgtt ttttggcaat
      361 atatcctcac cagcttgttc tgaagaccat gaggaggtca tttctaatca aacatcagtt
      421 atagattctc aaaaaacaga aatagaaact ttaaatagca aattgtctga tgctgaacca
      481 tggttcaaaa tgaaagacga cgaaaagaaa gctattgaag ctgaaaatca acgtaaagct
      541 gaagaagcta aaaaggctga agaacaacgt aaaaaagaag aagaagagaa gaaaggatat
      601 gatactggta ttacttatga ccaattagct agaacacctg atgattataa gtacaaaaag
      661 gtaaaatttg aaggtaaggt tattcaagtt attgaagatg gtgatgaggt gcaaataaga
      721 ttagctgtgt ctggaaatta tgataaggtc gtactatgta gttataaaaa atcaataact
      781 ccttcaagag tgttagagga tgattacata actataagag gtataagtgc tggaactata
      841 acttatgaat caactatggg tggaaaaata accataccag ggatagctgt agagaaaatt
      901 aattaatatt gttttgtatt atagttaata ttttatatta tagtcaatat gtttaaagat
      961 gtttttataa ttgcaaataa acagttacaa ggctctaaat tagtttttgc ttttagcata
     1021 ttatctattt tctatcaact attaattatt tagtattaat atttccatat atgaatttta
     1081 ttataaaata gtcaagaata atagattatt aaatgataga aaaattttaa ctaaaagtca
     1141 tgtattacaa taacacatga cttttaatta aatctcaata tttattatat aaaaataatt
     1201 tctgagtatc acaggaataa ttttttgtca aacatatatt ttagccatat atcccagggg
     1261 cttttactcc atcaacacca aagaaatata taacaccatc aatctcgaaa agtccaccag
     1321 ctgcagccat agcagtatca ggcataaagt aatatacttt actattaata gtttgccatc
     1381 cagtaactgc ttttgagtta ttaccaaagt aatatatttt tccaagtaaa tgtaggaatc
     1441 tattttgata acgtatagct tgaccatcta tattgttagc atccgtatta gcaggtgcaa
     1501 agtattcaaa tccattaggt cctttaaaca ctcctatctg aggtaaacca tttctaaagt
     1561 aaaaattttt attatcaata gttttataac cattcgcacc catagctgta ttaggctcga
     1621 agtaatatct attaccatca atagttgccc aaccagtagc cgcttttgaa tcattgccaa
     1681 agtaatatat attgtcatgt aaatatagga atctattttg ataacgtata gcttgacctt
     1741 ctatgttgtt agcatccgta ttagcaggtg caaagtattc aaatccatca ggtcctttaa
     1801 acactcctat ctgcataata ccatcagtat taaaataaaa atgtttacca ctaataattg
     1861 tataaccagt tgaagctata taagtgttag tattaaagta gtatttttta ccattaatag
     1921 tttgccatcc agtaactgca acagcagtgt tagtattaaa gtaatatttt ttaccatcaa
     1981 tagttcgcaa tccggtaact gcttttgagt cactaccaaa gtaatatttt ttaccattca
     2041 aagttaagaa tttattttgg taaagtatag cttgaccttc tatgttatta ttatgagtat
     2101 tagcaggtgc aaagtattca aatccatcag gtcctttaaa cactcctatc tgcataatac
     2161 catcagtatt aaaataaaaa tgtttaccat taataatcgt ataaccagtt gaagctatat
     2221 aagtgttagt attaaagtag tatcttttac catcaatagt ttgccatcca gtagctgctt
     2281 cagcagtgtt aagattaaag taatattttt caccatcaat agtttgccat ccagtaactg
     2341 caacagcagt gttaagatta aagtaatatt ttttactatc aatagtttgc catccagtaa
     2401 ctgcttttga gtcattatca aaataatatt ttttgccatt caaagttaag aatttattct
     2461 ggtaaactat agcctgacct tctatgttat tattatgagt attagcaggt gcaaaatact
     2521 caaatccatt aggtccttta aatactcctg ttaccatttt agattcatta ttagcatcaa
     2581 aatagaaatt atttctttca atagtaatat atccattttg aagaattcca tcataactaa
     2641 agtaatactt gtcgttattt atagtgcata gatgagtcgc agcaatagca ttattaggat
     2701 taaagtaata ttttttacca tcaatggttt gccatccagt aattgctttt gagtcactac
     2761 caaagtaata ttttttacca ttcaaagtta agaattta
//
LOCUS       DACTGW010000321         2796 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.326, whole genome shotgun sequence.
ACCESSION   DACTGW010000321 DACTGW010000000
VERSION     DACTGW010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.326"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(278..748)
                     /locus_tag="I9573_003512"
     CDS             complement(278..748)
                     /locus_tag="I9573_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HAT4798097.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
     gene            complement(1493..1882)
                     /locus_tag="I9573_003513"
     CDS             complement(1493..1882)
                     /locus_tag="I9573_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT4798098.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT          874 a          486 c          171 g         1265 t
ORIGIN      
        1 ttgaattact acatattaat atagaaattt ccgataataa actttatacg tttaagtatt
       61 cctacttcaa attaaaaata ttataaatat tcaaatttat taatatattt taatattata
      121 tttatttttt ctttattaat attcccctta tttttcttta ttaatatttt ctttatttta
      181 atttaattta tgacatttgt caaatactag tcctatatat tatcttgaat tagttccact
      241 acaattaatt tctactaaat aattataatt taaacaatca ttccttacta ttttcacact
      301 gctttacaaa ttcaattgga tgttgaatta ttttagaagt ttcttctaca gagtatccct
      361 cagataaata tcttctagct ataacttcca tagactctcc tatctcaata atatgcttat
      421 ctgggtcata aatacgcact actctttgtt gccattcatg ttttagtggc ttatggacaa
      481 actctacatc tttataataa ttgagttttt caataaattc gtcaaaatca tcaacctcaa
      541 aataaagttc catattattt gatttttgta taattgtatc tggattaatc cctgtaagcc
      601 atgcgaagtc ttcttgtata gcaaatcctc ctttaaatgt tacatttttg cctaagtcca
      661 attcaatttc ttgctcaaat aattctttgt aaaatttttt tgatacttca atatccttca
      721 ctgctaataa tgaaacctga tatttcataa attttcctcc taatttttta tttctatttt
      781 ttcagtatct tctattaaca tttttttctc tatctcttca gtacctttta ttagtccttt
      841 atttttactt ttttagtatc ttctattaac acttttgttt ctacctcttc agtatattat
      901 atcaatcttt tatttttatt tttcaatatc ttctattaac acttttatct ctatctcttc
      961 agtacctttt attaatcttt tgtttttatt ttttcaatat cttctattaa cacttttgtt
     1021 tctacctctt cagtatcttc tattaatcct ttatttttac tttttcaata tcttctatta
     1081 acacttttat ctctatctct ttaatatctt ctattaattc tttattttta ttttttaaat
     1141 atcttcatta attcctattt attttatcat cttaaatacg aaaacactgt gtcatatttg
     1201 caaaatatat attgtcatct atattaaagc ttcacaattt atatttaaaa attctcttta
     1261 atcacactac ctctatttaa aaagctacct taattatact tataagtaaa ggtagctttt
     1321 taaatttaat taaatagaat atccactatc aatttatatt taatttattt agattcacat
     1381 tatagtatca taatccactt ataatcattt gtcatattac ttaagtagac taatatatta
     1441 cataaataca cttacataca ctttatatta atgctataag aaatacgttt gcttatacgt
     1501 ctttctcctc ttcttttaga ttttttatcc actcttcaat aagtaagata tcatctttgc
     1561 tcaaattaat attttcatgt aatgacaaca ataaactttt tatagaagaa tcatgaatat
     1621 ttctaaaaaa atctcttgtc tcaactccta tgtattcctt ttctttaatt aaaacttcat
     1681 aatgtgtata tttatctatc ttttgagaat ttagaaatct ttttactact aatctagata
     1741 aaagagtcaa agttgtcgtt tgtttccatt gatatttttg ctccattcct aaaacaatat
     1801 ctctagatgt tactttggaa tctgatttcc atataaattt cattatcttt aattctgatt
     1861 gtggcaactt ttttatagtc atgataaaac cttgctactc agaatataga caagcaatat
     1921 ccataataaa atttttcgat taaatacatc ttatacggat ttatataagc tcagacttat
     1981 aagatatttt tcaatcttaa tcctaaattt agctatttgt acacggtaaa tatatataaa
     2041 taatgtcaaa attctcttgt attatattgc ttaattttat gagatatttt gtactcagct
     2101 atctattttg gatacttgtc cagtagctct cctttctttt aaactctttg attttaaata
     2161 tttgtatttc agtaaatcct acttcctaaa ttttttattt actacaactt attactgaac
     2221 ttcctacttc ctaaactttt catttactac aacttattac tgaacttctc acttactaaa
     2281 tttcttactt actataaatt ataactaaac ttctcactta ctaaatttct tacttactat
     2341 aaattactac taaacttcct actttctaaa tttcttactt gctataaatt attactaaac
     2401 ttcctacttt ctaaatttct tacttactat aaattattac taaatttcct actttctaaa
     2461 tttcttactt actacaaatt attactaaac ttcctactcc ctaaatttct tacttgctac
     2521 aaattattac tactaaactt cctactccct aaatttctta cttgctacaa attattacta
     2581 aacttcctac ttcctaaatt tcttacttgc tacaaattat tactaaactt cccactttct
     2641 aaatttctcg cttactacca cttatcacta aacttcctac tttctaaatt tcttgcttac
     2701 taccacttat cactaaactt tttacttctt aaatttctta cttcttaaga ataatgttaa
     2761 ttttaatttt aagattatag tttttgcctt gtttaa
//
LOCUS       DACTGW010000322         2776 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.327, whole genome shotgun sequence.
ACCESSION   DACTGW010000322 DACTGW010000000
VERSION     DACTGW010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.327"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..811
                     /locus_tag="I9573_003514"
     CDS             <1..811
                     /locus_tag="I9573_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAT4798099.1"
                     /translation="FGNIDEGISCYIKGLNLVKKSSLNLRKISIFYGLIANGYELKKD
                     FKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLVDDNKKLENDIL
                     KDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGHLAGDKVIVKVT
                     DIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRVFKLLEFEGIEH
                     IYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQYIII"
     gene            828..>2776
                     /locus_tag="I9573_003515"
     CDS             828..>2776
                     /locus_tag="I9573_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4798100.1"
                     /translation="MRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLMENQ
                     GRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVVLIL
                     GDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACSFAR
                     QYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAIDSNG
                     SIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINNLIV
                     NTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKDKLT
                     REFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAENLL
                     ESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEERQIM
                     RIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRREDIIP
                     LFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYVVTG
                     KIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKEVNK
                     YVEEKIISMLFAQGLSKNEVAQVLG"
BASE COUNT         1162 a          238 c          530 g          846 t
ORIGIN      
        1 atttggtaat atagatgaag gtataagttg ttatataaaa ggtttaaatt tagttaaaaa
       61 gagttcattg aatcttagaa aaatatctat attttatggg ctaatagcaa atggatatga
      121 gctaaaaaaa gattttaaac tggcaataga atattataaa agtatggatt ggtattctga
      181 taggtgggaa aaaaataatt cctataaaac atctcaagca attcacaaac aatatgaact
      241 cagacaaaaa caagagagtt taagattact agtggatgat aataagaaat tagaaaatga
      301 tattttaaaa gatggactta ctaaactata taatagaaga tatttggaac agcagttgga
      361 tttatttaat ggaaccagag acaaaaatat ggttgcaata ctagtcgata tagattgttt
      421 taaaagttat aatgataatt atggtcatct agcaggagat aaagttatag ttaaggtaac
      481 tgatattata agaagtgtat ttatagatat taccgaacat ataatcagat atgggggaga
      541 agaaatttta atactagtag attctataga atcaatcggt gttgaagaga ttgataatat
      601 agaattatat attaaaagag ttttcaaact tcttgaattt gagggtattg aacatatata
      661 ttctaaaata aaaaactata taactataag tgctggagta agtataaaaa gatgtcgtag
      721 tagaaaagat gttgaaatat taattgaaga tgcagataag aacctatata aatcaaaaaa
      781 agcaggtaga aatcaataca ttataattta aaattgaggt gattttgttg cgaaaaaaaa
      841 taggaattat agcctctgat attgaattaa aagaaagaat agaagaatta tatagagaag
      901 atgtagaaaa tggaactata ataatcgaca tacttaacct agacttaatg gaaaatcaag
      961 gtcgtatact tgttgaaaaa ggtgcccaag ctataattgg tagaggtgga ggttatagtt
     1021 tagtaataga tacagtaaat gtacctgtta taccaatgaa tatgaagtca actgaccttt
     1081 taagagcaat agaaatagca aagaaatatt ctaaaaaagt agtattgatt cttggagaca
     1141 atgaagtttc ttttgactat gttggctgga gaaatgtcat aagtactgaa ataactgaag
     1201 aatggtttga atctaagtat gagataagaa gtaaagttgt aaaatatata gaccaaaaag
     1261 atgaagttgt aatcgtagga ggaggtttgg catgttcttt tgcaaggcaa tatggaattg
     1321 atagtgtatt tgcaactgct agtgatgaat ctataagaga agctgtagaa tattgtaaaa
     1381 aattattgga tacattaggt gaagaaaagt ttaataatga agtgttacga aatatacttg
     1441 atggtataaa agatggagta attgctatag atagtaatgg tagtataatt ttgtacaatg
     1501 aaagtgcaaa aaacatgctt aaagtagaga gaaaatgtgc acttaataaa tacatcttag
     1561 atgtatttcc taaaatggag tggatgttag attgtttaca tgaaaaagag gatgtagaag
     1621 atagaaagat aagaaatata aataatctaa ttgttaatac taggacaaca ttaataaaag
     1681 ttgataatag tacttatgga gttttaggaa taatacaaga tataaccaag ctacaaaact
     1741 tagaaagaaa aataagattt gacttgaatc aaaaaggtct ctatgctaga tatacttttg
     1801 atgatttttt atttaaagat aaattaacta gagaatttat tgaagaagct aaaaaaattg
     1861 gaaaaagtga ttatacaact ttactttatg gagaaagtgg gtctggtaaa gaaattatag
     1921 ctcatagtat acacaatata agtaaaagaa aagataggcc ttttgttgct ataaattgtg
     1981 ctactatagc agaaaattta cttgaaagtg aactttttgg ctatgaagaa ggtgcattta
     2041 ctggtgctag aaagggtgga aaaagaggac tatttgaact tgctcatgga gggactcttt
     2101 ttttagatga aataaatagt ctatccttta atattcagac aaaactatta agagtaatag
     2161 aggaacgtca aataatgaga attggttctg attatataat acctctggat ataaggatta
     2221 tagcagcaac gaacgaatct ttaaccgaaa aaatagttat gggtacattt agagcagact
     2281 tattttatag attgagtagt ttagagataa atatacctcc tttaagagac agaagagaag
     2341 atataatacc tttgtttaac aattttgtaa atgaagtatt aaaggatgat ggtctcaatg
     2401 gaataaacag tatagatgaa aattttgttt tgactaaaga tgaaatggat aagctatata
     2461 attatagctg gcctggaaat gtaagagagt taaagacaat agctcaaaaa tatgttgtga
     2521 caggtaaaat taaattgaga caagatagag attttaaaac taaaaaatta cttcctaact
     2581 ctgaagtaga caaatttaat tctgaaacga cagcaagtgt agaagtccaa gatgaatcta
     2641 taaatataag taagataaat gatggtaaga taagtataga tataaaagaa gtaaataagt
     2701 atgttgaaga aaaaataata agtatgcttt ttgctcaggg actttctaaa aatgaagttg
     2761 cacaagttct tggaat
//
LOCUS       DACTGW010000323         2769 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.328, whole genome shotgun sequence.
ACCESSION   DACTGW010000323 DACTGW010000000
VERSION     DACTGW010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.328"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            319..2454
                     /gene="rnr"
                     /locus_tag="I9573_003516"
     CDS             319..2454
                     /gene="rnr"
                     /locus_tag="I9573_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAT4798101.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILAKILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1212 a          264 c          499 g          794 t
ORIGIN      
        1 cttatatgag atgaatattt taccaatcct atttgatggt gtataattat gagataaaga
       61 gtaatttaag caattatgat aaagaataac ttaagtaatt ataatgatat ttacaaataa
      121 tttataaaac atcataattt gtttgaagtt agtatgacaa agcttacatt aaatcaaaaa
      181 tataaataag taattttaac ttgtaataat aataagaaaa tatagaaaat tatgtgataa
      241 gtaaaaaatg ttttatctga agaaatataa aagacaatat aaataataat ataatgaaaa
      301 gtaaaacagg agggaaaaat gataccagga cttaaagaaa gtttgctagg attaataaat
      361 gaaagtgcat ataatcctct aaaaaaagaa gaattagcag aaatatttga tatacattca
      421 tcagaaatgc caatgtttta taatttttta aatgaattag aagaagatgg ttatatctgc
      481 tttactaaaa aaggaaaaat agtatctccc aatcaaatgg gatactttgt tggaaaattt
      541 gtatcacaca aaaagggttt tggatttgta gaatctgatg ttgaatatac acaggattta
      601 tttatctcaa gtgacaatat aaatggtgct atgcataacg atagagttat ggcagaaata
      661 gtagtacctg caactgaaga aaagagagca gaaggaagaa tagtaaaagt aataaagaga
      721 gaaataacta gaatagtagg tactttccaa tcaagtaaaa cttttggatt tgtaacacca
      781 gacaacaaga aatttactaa agatatatat atacctaaaa aacattttag tggagcagta
      841 gataatgata aggtagtatg tgaaattaca gtatggccac aagaggatag aaaaccagaa
      901 ggcaaaatta tagagatact aggtcaaaaa ggtgaaagag gcgtagaaat agattctata
      961 ataagagaac atggtcttcc agaagaattt ccaaagaaag tattgcaaga agcagaagct
     1021 gtagctgttg agattcctca agaagagata aaaagaagaa gagatttaag agatttaaac
     1081 atatttacta tagatggaga tgacgcaaaa gacttagatg atgccatatc aatagaagta
     1141 ttacctaatg gaaacttcaa actaggagtt cacatagctg atgttacaca ttatgtaaga
     1201 gagaaaaata aacttgataa ggaagcctta aaaagagcta catcagttta tttagtagat
     1261 aaagtaattc caatgttgcc aaaaactctt tcaaatggag tatgtagctt aaatccattt
     1321 gaagataagt taactctttc tatatttatg gaaatagacc ataaaggaaa tgtggttaaa
     1381 catgaaatat gtgaatctgt aataaattca aaagctagaa tgacttatac agaagtatct
     1441 gatatattag aaaaggatga tgaaaaactt aaaaagactt ttgaacatgt tgttgaagat
     1501 tttaaaaatg ctgaaatttt agctaagatt cttatgtcaa gaagagaaaa aagaggagct
     1561 atagacttca atttcccaga ggcaaagata attctaaatg gaaaaggcga agttgtagat
     1621 ataaagccat atgaaagaag aatatctaat aagataatag aagaatttat gcttataagt
     1681 aatgagacaa ttgccgaaca tttttactgg atgggaatac cgtttgtgta tagaatacat
     1741 gagacaccat ctttggaaaa aatggaagaa ttgagtaagt ttatatctac atttggatat
     1801 ataataaaag gtgataaaga ggaagtacat ccaaaagcat tacaaggtat aatagagaaa
     1861 ataaagggca aaaaagaaga aggagctatc agtactataa tgcttcgttc tttaaaacaa
     1921 gctaagtact caccagaatg tagtggacat tttggattag cagctaaatt ctactgtcac
     1981 tttacttcac caataagaag atatcctgac cttcaaattc atagaataat aaaagaatct
     2041 ttaaataata aaataagtgg aaaaagacaa gaacagctta ctactatagt ggattatgca
     2101 tctgttcaat catccgagaa agaaagaaag gcagagttag ctgaaagaga tgtacatgat
     2161 ttctataagg ctctttatat gaaggataaa gttggtcaag aatttgaggg tgttgtatct
     2221 agcgtaactt catttggaat atttgttgag ttagataata ctatagaagg attgataaga
     2281 cttgcaaata tgaatgatga ttactatatt tatgatgaga gttcttatag tgttttagga
     2341 gaaagaacta agaagtcata taagatagga gaccttgtta agataaaggt tgaaagtgtt
     2401 aatgtagatt ttaaagaaat tgactttgag atagttgaaa aaatagagga gtagataaaa
     2461 cagctaaaat aagagataac aagttgtagt atttatgata tgtataaata tatagtgcac
     2521 aatactctat aaaataaatg ataaaaatag gtaattaaga aatatctaat ggaaatagtt
     2581 aacttaaaga taaataatta agaaatattt aatgaaagta gctaattgaa aaataagtaa
     2641 ttgagaaata tttaatagaa agagctaact gaaagataag taattaagaa acatttaata
     2701 gaaaaagcca actgaaaaat aaataattaa gaaatgttta atggaagtga ctaattgaaa
     2761 aataagtaa
//
LOCUS       DACTGW010000324         2725 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.329, whole genome shotgun sequence.
ACCESSION   DACTGW010000324 DACTGW010000000
VERSION     DACTGW010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.329"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            8..910
                     /locus_tag="I9573_003517"
     CDS             8..910
                     /locus_tag="I9573_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HAT4798102.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIATF
                     GKLLIPFIMAGILGIVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            1519..2532
                     /locus_tag="I9573_003518"
     CDS             1519..2532
                     /locus_tag="I9573_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HAT4798103.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKTFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFEIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
BASE COUNT          952 a          252 c          484 g         1037 t
ORIGIN      
        1 tggaaatgtg ttgccacaag attattatgg attaatagtg attttaggtg ttattcttgg
       61 agttatgggg gcattttaca actggtttac tctatatgtg cagtctttat acaagaagat
      121 tcctaaaatt gctacctttg gaaaactgtt gattccattt ataatggctg gtattcttgg
      181 gattgttatg cctgatgttc tggggagtgg acacaattta gttgaagaac ttacaagtca
      241 tgaatatatt tttggaatgg ttgttttgat ttttgttata cgttttatat tttcggctgt
      301 tagttttggg tcaggtgcac cagggggaat attttttcca ttgcttgttt taggagcatt
      361 tataggtgga atatttggta tggtaggtgt taaagttttt ggaatgaatc ccgattatgt
      421 aaataacttt gtgcttttgg ctatggcagg atattttaca gctattgtgc gtgccccttt
      481 gactggtatc atattaattt ttgaaatgac aggttctgtt agtcaaatgc tttcactatc
      541 agtaatttca attgtggcat atattgtggc tacacttatg aagtctaaac ctatttatga
      601 aagtcttctt gagaggttac ttgaaaaaaa tggagaacct gtacataaag agaggggcga
      661 aaagatacta gaccagttct ctgttatgtt tggctctcct gttgcgaaca aggcaattca
      721 ggatgtacag tggcctgaaa attgtctttt ggtagcaatt caaagaggtg gagaagaaat
      781 tattcctaga ggtaagacaa ttcttagacc aagtgatgta attgtgacta tgacagatga
      841 gagagatagt ggtgctgtgt atgataagat ggaaaatttg tgtaaagaac atacgtcaca
      901 tattttttag ggataatgat gaatgtttaa taatgataga atatttaatg aagatttgat
      961 taaaaaattg gtaatttgaa agagagttaa ggaattaata gaaaatgaaa atgtgatatc
     1021 ttagatgtaa aagagtttga agaaaataat tgtatatatt tgttaatgag attatatgga
     1081 ctaatggtat atttatactg tgctaaaagt tgcactattg tatgaggaat gggaataaag
     1141 ggtttgaaaa aataggtaga agatttttca gatattctta ttgggatatt ttaattaata
     1201 tattttaatt gcgatatttt gggaagaata gaatttattt ataaatgaaa ataattaaaa
     1261 ggggttaagg ttgtattagc cccttttgct tatagagttt ggaaatggta tcttgatagc
     1321 agttgtttta tgcttattat attttattac atattttaaa attaacctta tcactcctaa
     1381 atcattattc tatcaacttt taaaacactt tcaagacaat cacctattga aaacaaagtt
     1441 aattaaaaac ctacatatca gtttagtatt cgtgtagatg attatataaa tataaaagta
     1501 taagaaaggt gagtatctgt ggttagttca tttttaaatg atatctttag aacaatttta
     1561 gtaagttgta tttttatagt tattttatta gtctttagaa taactttatt taagactttc
     1621 agtaaaaaat ttaattacta tatatggctt attgtgatta ttaagctatc attaccattt
     1681 atgaattata cttttatatt taatgagtca aaatataatg gtaatattaa taagataagt
     1741 ttaggcaatt ttaatagtat ttctattgta tatagtactg ttttagtctc tgtttggatt
     1801 gctattacga tattttattt agtttatact ttattgaaat atattaagct taaaaatatg
     1861 ataaatgatt tgtcttatga tgttgaggat gataagataa aaaatcttta tgaaaattta
     1921 ttaaaagagc ttaagataaa taaaaaaata cggcttaaat attcatatga agttgaaagt
     1981 ccagtatttt ttaattcgtg tgtcatttta ccaccatgtg attatacatt aaaagagtta
     2041 aactggatat ttaggcatga gttgatgcat tttaagagta aagaccttta cattaagtat
     2101 ttggtgatat ttttaaaatg tgtttattgg tttaatcctt ttgtgtatat aatggagaag
     2161 tttatagatt tggattgtga attttattgt gatgaaagag ttttgaagaa ttgtacgata
     2221 gaagagaagc aggattatgc attgacaata attagtgcaa tgagaaaagg ttcaaatagt
     2281 tcaaataaat ttgtggctgg attacataag gagtcagata ttaaaaaaag agtgtttagt
     2341 atgtttaatg aaaagtacag aaatggtatt ttgatagcag ttattttatg tttattatct
     2401 tctattacat attttgaaat tgatttaatt actactaaat caatatttta tccactttca
     2461 aaacaaacac ctattgaaaa tagaactaat tcaaaaccta catatcagat taatgtttat
     2521 atagataatt aaaaaataca aagtttaaca agagattaat ataaataatt ttaataagta
     2581 tctttcacat atgttaaatt tactttaatt atattaaagc caatgaatta ttctttagaa
     2641 gctccaatgc cagtaaatac aggtaaagaa aaatctttta ctacaattgt aaattaaaaa
     2701 atattctaat atttaaagaa atgca
//
LOCUS       DACTGW010000325         2717 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.330, whole genome shotgun sequence.
ACCESSION   DACTGW010000325 DACTGW010000000
VERSION     DACTGW010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.330"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(317..1213)
                     /locus_tag="I9573_003519"
     CDS             complement(317..1213)
                     /locus_tag="I9573_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT4798104.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDFLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1355..>2717
                     /locus_tag="I9573_003520"
     CDS             1355..>2717
                     /locus_tag="I9573_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAT4798105.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTL"
BASE COUNT          932 a          385 c          316 g         1084 t
ORIGIN      
        1 aaaaatcata ctattttagc tgtttacttg cttatttgat agttttattt ctttgattaa
       61 atatctttca gaatctaagc cagatgtctc aaaaaaactg atttataata taatcatttt
      121 ttctgctatt cttatttttc caattttagt ttcatattaa aatatacttt tcatagacac
      181 tacattcttt catactcatt ttcacattta actatacaag tagtcttact catttatgta
      241 ctatacttta ttaatattta ttaagaaaaa ctcaaagatt taaaaatata tattttctat
      301 aaagtatatt tatgaattat ttcatatctt cttttaaaat aacattacta acttctctat
      361 gttcatttct atacttagtt ggagatatat tataattcct tttaaataag cgactaaaat
      421 aatttatgct atcaaagcca catctcatag caatttctgt catatttaaa tctgtttcct
      481 ttaaataata tatagcttta tcaagcctga ttccatttat aaaatcgatt gtagtcttac
      541 ctgttatttg cttaaatgtt ctacaaaagt gatgtgcact caagttcaca cgactagcaa
      601 gttcttttaa tgtaatttta tccatatatt tttcttctat caagtcaaag atacattcaa
      661 atttttctaa agaatacttt ctttttataa attcatcttc agaatatatt ttatcaatat
      721 gacttcttaa taagagtact attaattgat agatattagc ttttatagat aactcatatc
      781 caacttcttt attgtgatat tcatcaagta tagttgtaat acattttaaa atcagaaaat
      841 cattttgaat taaattatta aatacaatat gatttaaagc caatggtgct aagtacttag
      901 tttgtaacaa atcaacttga ttactaaaaa ggaatgtggg ctcaatacga atactataga
      961 attttaaatt atctgataag ctttctaaat aatgtaattc attactgttt gctattacaa
     1021 tatttccagg cactacctta aaattatcca atccacattc aagatatgct ataccatcaa
     1081 taaaataata tatttgtaga tgctcatgcc aatgtctttt aaagtttgct ccttttttaa
     1141 tgtcatcctt taaaaaaata tctataaagt tttcttgacc tttcctatta aaatatgtat
     1201 ttttatcctt cattattccc ccctcatcaa aatagtgcta attataagca atatagttat
     1261 tgtattcatt atttcatatc gctattatat aaaatgtaac tatcattgta aacattaaca
     1321 taatgccagt cattataata aggaggtaat aaaaatgaca aactctttta aaaaagatga
     1381 taatagaaaa aaaatcacag ttttatttgt agtcattgcc atgactttta tggcaacatt
     1441 agattcgagt attataaatg ttgctctccc agttcttgca agcaaactaa atgtatctct
     1501 agcatctatt gaatgggtaa tagcaagtta ctcaattatt atttgttcaa cactattatt
     1561 ttttggaaga cttggagata ttattggtaa atcaagagta ttccaagtag gtactattct
     1621 ctttacatca gcttcattac tatgtggatt aagtaattct ttaactttac ttatagtttg
     1681 ccgctttatt caaggaatag gcgcttctgc ctacatggca aacaatcatg gaattatcac
     1741 agaactattt ccaaaagaaa gcagaggaaa agccctaggg attctagtta ctgcagttgc
     1801 tattggaaat atggttggac catctgtggg aggatttatt ttgtcaatat ttgattggaa
     1861 tgttatattt tttataaata ttccaatcgg tcttattgta atctttttaa atacaaaatt
     1921 tcttcctaat agtaaaaaat cttcagaaaa tatggataaa actggtgcta tacttcaatt
     1981 tttaggaaca acattatttt ttagtgctct aattagtgct caacaaacag gattactaaa
     2041 tccatatata ttaattgcac tattattatc aattattttt attatattat ttttgatatt
     2101 agaaaaaaaa catcctcaac cgcttcttga tcttgaaatt tttagaaatt ttaaattttc
     2161 attgaattta atttgtgctc ttacatcatt catttgtatt gcatcatcaa gtattttaat
     2221 tccattttac ttacaaagta ctatgaaact tccccctatt caagcaggtt tatttatgat
     2281 actatcacct ttgattttag ccattttttc acctatattt gggaatatat cagataaaat
     2341 caagtcagaa aaaattattt taattggtct tctcgttatg agttttggat tctttctaat
     2401 gtcaagattg aaagaaagtt ctgcccttat tttatttgta atttatatat cgataatatc
     2461 aataggacaa gctatatttc aacctgctaa caatgctttg ataatgtcaa gctgttctag
     2521 aagtaagcta ggagttgtag gaagcataaa ttcccttgta agaaatttag gacaagttat
     2581 aggtattact atttctacaa cacttttata taattttatg agtattaaag ctggatatag
     2641 ggtcaatgat tatgtgatta ataatgataa aatatttgta tttggaatga gaaatgttta
     2701 tattattgtt acacttg
//
LOCUS       DACTGW010000326         2709 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.331, whole genome shotgun sequence.
ACCESSION   DACTGW010000326 DACTGW010000000
VERSION     DACTGW010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.331"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            170..313
                     /locus_tag="I9573_003521"
     CDS             170..313
                     /locus_tag="I9573_003521"
                     /inference="COORDINATES: protein motif:HMM:NF014609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798106.1"
                     /translation="MNKKFRLYQVDSFTKERFTGNPAGVITNADGLSESQMQKITREL
                     NNS"
     gene            335..1090
                     /locus_tag="I9573_003522"
     CDS             335..1090
                     /locus_tag="I9573_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HAT4798107.1"
                     /translation="MAKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAVENQFNN
                     KTVFQKTGAGILPVDIIKKDNDYEIIMTQGKIEVSPPLQQTYQKRLLNAFGILNEDIR
                     TDCPIAISSTGHSKVMIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHNKETL
                     QVHGRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPIPNTEFIFEAVQGEAIKRKGK
                     MLVKVIISDNKPVEVKIIGKAVIAFSTNIEIEL"
     gene            1315..1926
                     /locus_tag="I9573_003523"
     CDS             1315..1926
                     /locus_tag="I9573_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAT4798108.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIIEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            2224..2562
                     /locus_tag="I9573_003524"
     CDS             2224..2562
                     /locus_tag="I9573_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HAT4798109.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
BASE COUNT         1087 a          313 c          345 g          964 t
ORIGIN      
        1 tagcatagag ccttacataa tactactttt ttagatgtat atttttaaat tttaatatag
       61 acatatgtta ttttattaat tttatttaaa cagataataa taaataataa tttatctcaa
      121 gttttctatg tagataaact aatctaatgt ttagaaagga ttatataata tgaataaaaa
      181 atttcgtctt tatcaagttg actcatttac aaaagaaaga ttcacaggta atccagctgg
      241 tgtaattaca aatgctgatg gcttatctga atcacaaatg caaaaaataa caagagaatt
      301 aaataattca taaactgctt ttattttcaa taaattggct aaaaatcatc aagaaaatta
      361 tgatgtacat atcagatttt ttacaccaac taatgaagtt cctatatgtg gtcatgctac
      421 tattgctgcc cattatgtta gagcagtaga aaaccaattc aataacaaaa cagtatttca
      481 aaaaactggt gctggaatat taccagttga tattataaaa aaagataatg attatgaaat
      541 aattatgaca caaggaaaaa ttgaagtatc tccaccatta caacaaacct atcaaaaaag
      601 attacttaat gcatttggta ttttaaatga agatattcgt acagattgtc ctattgccat
      661 atcatctaca ggacattcaa aagttatgat tggaataaag gaattatcaa cattaaatag
      721 tttatctcca aatttatatg aattatcgaa aataagtcaa gaaataaaat gtaatggata
      781 ttatattttt acattacata ataaagaaac tctacaagtt catggaagaa tgtttgctcc
      841 tgcaattggt atttcagaag accctgttac aggaaatgct aatggacctt taggtgcgta
      901 ccttgtacat tataaagtaa tacctatacc caacacagag tttatatttg aagcagttca
      961 aggagaagca ataaaacgca aaggcaagat gttagtaaaa gtaattattt cagataataa
     1021 gccagttgaa gtaaaaataa ttggaaaagc tgtaattgca ttctcaacaa acatagaaat
     1081 cgaattatag attctttcta agtaaatttt tgaacttttt aatactaatt taataacaaa
     1141 ctaatttata tacaatcaaa ttaatttttt tattatttgt taatatatat ttatcattat
     1201 attatataat aatatagata gttactatat cttataatta gataaatcat aattatatat
     1261 tattgttaaa actcaattat ataattttta ataatattat tttaggaggt aaaaatgata
     1321 aacgatatta aaaataaatt caaaaattac aaaccatata taaatggata caagaatatg
     1381 aagagagcat ctgttttaat tccaataata gaaataaata acacacacta tatattattt
     1441 gaagttcgct caaaaaattt gaagcatcaa cctagcgaaa tttcttttcc aggtggtaaa
     1501 atagaatcag gtgagactcc atatgaagct gttattagag aaacttgtga ggaacttgga
     1561 acttttagtg acaatataga agtaatatca agtcttgacc tccttataac accagtaaag
     1621 tttatcatac atccatactt aggatatata aaaaatatta ataatttaaa tataaacaag
     1681 gatgaagttg accatacttt tttggttcct ataaaatatc ttttagagaa tcctccaaat
     1741 acttatagta atactgtaaa agtttttcca gatgaaaatc taccatatgg ttcaattcca
     1801 aaccaaaaag attataaatt tgaaagtgga agttatccta ttctgtttta taaatataaa
     1861 gattatgtaa tttggggaat gacagcaaaa atactagaaa actttttaaa ttttttatca
     1921 gaataacact cataataaat catcaaaact tttacataag aaatttataa aagtttctaa
     1981 atttaacaat tctaaaatat caataactat attttgtagc acttttttat tttattattg
     2041 tttgaatatt tgatggtttt caatgcaatt tcaacataaa tactatgtat tagtgtatac
     2101 tgttgatgaa ttaaagtatg ataatctatt taataaagta tgaagtaaat gattttaatg
     2161 tgtctttatg gtaaaattac taataagtaa taaaattaat taaaatatat ggaggtgctt
     2221 ataatgtttg ctgtaggatt tgttgttata attgcacttg cctttttcat tttaagtgta
     2281 ctattattta atggaaaagc cagtatgcta ctagcaggtt ataactctat gagtaagaaa
     2341 gaaaaagcac gatacgataa aaaaaaatta tgtagaagtt ctagcattgt tacttttatt
     2401 gtctctataa tgctatttat tatggcattt ttgggatata gaatagagag tggtcaaatg
     2461 gacgaaaaaa aaatggttcc cttttctata atatttataa tagtcatact ttccagtgta
     2521 ttttttaata tcctctactc taacaaaaaa tgtaaaaaat aaatagttgt aagttgagca
     2581 gttaattata aaagattgac tgctttttca tgtatattga ctactcttat taaatatttt
     2641 aacatttaaa aatctatgta aaaataatga atatagtaca aacttctaat aatgatttta
     2701 cctttgtgg
//
LOCUS       DACTGW010000327         2698 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.332, whole genome shotgun sequence.
ACCESSION   DACTGW010000327 DACTGW010000000
VERSION     DACTGW010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.332"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            103..1323
                     /locus_tag="I9573_003525"
     CDS             103..1323
                     /locus_tag="I9573_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4798110.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1360..>2698
                     /locus_tag="I9573_003526"
     CDS             1360..>2698
                     /locus_tag="I9573_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT4798111.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYSLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWFIRIISWSTL
                     AVGFATALGSFWPESATEYKGCIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFVIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     IALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISRE"
BASE COUNT         1016 a          345 c          459 g          878 t
ORIGIN      
        1 attcaatatt cttataaaaa acaaaattct ccatcgaaaa ttatggcata ataattgctt
       61 attatattaa taaaacaata aaaagcaatt ggaggaataa aaatgcaaca ggctttgaaa
      121 ttaaaatatc aaaccaaaga attaggaaaa gattttgtaa tagaaagttg taattctata
      181 aaaccatggc ttataaacat aagaagagaa ttacataaaa ttccagaatt agctcttgaa
      241 gaaaatctta ctaaacagaa ggtaatttct tatttaaaag aaatcggaat tgattatatg
      301 gaatttacaa aacacaatgg aattatggca tatattttaa aagaaagtgc agataaaacg
      361 atttgtataa gagcagatat ggatgctctt cctattgaag aagaaaacaa cataccttat
      421 aaatcaatac attctggaaa aatgcatgct tgtggtcacg atgcacatac aaccatgcta
      481 cttggagcat gtaaagtttt acattcaata aaagataaac ttaatgtaaa tgtaaaattc
      541 ttatttcaac cagcagaaga aggttttgga ggagcaaaat ttttagttga ggatgggtgt
      601 ttagaaaacc ccaaggccga ttatatattc ggacttcatg ttatgccaca tatagaaaca
      661 ggccttatcg aaacaaaata tgatacatta aatgctagtg tagacactat aaaaataagt
      721 atcaaaggta aaagagctca tggtgcatat cctgaaaatg gtattgatgc aatagttaca
      781 gcttcacaaa ttgttacatc attacaaact ataataagta gaaacttaga accaaataat
      841 gctgccgttt tgactatagg taaaatatat ggtggtgatg cacacaatgt aatatgtgaa
      901 gatgtaaaac tagaaggcac tttaagaacc ttaaatagca aaacaagaaa ctttatgatt
      961 gataaaatag ctaaaattgt tggagacact gcatctgcat ttggttgtgt tgggacttta
     1021 catgtaagtg atgaaaacta tcctgctgtt ataaatgaaa aagaattagt agatacagtt
     1081 atatcaagta ctaaagaatt acttggagag gaaaaattta tattgagacc aaacccgtct
     1141 ttaggtggtg aagacttttc tttttacaca gaacattgta aaggagcatt cttccattta
     1201 ggatgtaaaa atgaggaaaa gggcttaatt tcacctcttc atacctctag cttcaatata
     1261 gatgaagatt gtttaccaat aggtgttatg atgcatgtaa tgaatacttt gtattttaat
     1321 tagtacagta attaataaaa gaataggaga taaattaata tggaaaaagc taagaaatta
     1381 ggtctattta gtatgatatt attagggatt aatagcataa taggttcagg tatattctta
     1441 ctaccaggta aagtttatag tctagcaggt caaaacagta tgtttatata catttttgct
     1501 acactcttag ttctatcaat acttttatgt tttgcagaag ttggaagcat gtttgataag
     1561 aatggaggag catatctata ctccaaaaaa gcttttggag attttatagg atttgaagtt
     1621 ggtacaatgt cttggtttat tagaattata tcatggtcta ctctagcagt aggatttgca
     1681 acagctttag gttcattttg gccagaatct gccactgaat acaaaggttg tatagctgca
     1741 atactcgtaa cactactttc aataaatagc ttatttggaa ttaaaagcac aaagataatg
     1801 aataatgtaa taactatagc taaattagta ccattaatag tttttgtgat agttggaata
     1861 ttctttataa aatttgtaaa tattgttcct tcaggaaatg tagttaactc tagtatgggt
     1921 ccagccatta tattagtatt ttacgcattt accggctttg aatcatttat tgtggcaagt
     1981 ggagaaatgg aaaatcctaa aaaaaatctt ccaatcgcat taataaccac aatatttata
     2041 tgtgcaataa tttatatatt aattcaaatt gtctgtatgg gtatacttgg agatagacta
     2101 tttgaaaaca gtattccaat tgcagatgct tcaagtgtat ttttaggcaa ttatggtaaa
     2161 gttttcatat ctgtagcaac attaatatct atatttggta taaatatagg ttcatcaata
     2221 gtaactccta aatgtggttc ttctctagca gaagaaggtt ctcttcctgc atttatagga
     2281 aaaacaaata aatatggtgc tccatatgta gcaataataa tatctttaat ttgttgtata
     2341 ccacttgtgc taactggaag ttttgaacaa cttgcagtaa tgagtgtaat tgcaagattt
     2401 gctcagtaca taccaacttg tttatcagta atagttttaa gaaaaagaac agatgttaag
     2461 gcctctttta agattccatt tggtcctgtt ataccaatgg tagctatttt aggtagctta
     2521 tggttgttac aacaagcttg ggcagaagat atttcaaaac ctataactca aaatagagtc
     2581 ttaataggat taggggcaat gttgttaata gctcctctat atatatttat gaaaaaaaat
     2641 aaggaaatcg agcataaaaa taaatcagaa gattctaaat tatatatttc gagagaaa
//
LOCUS       DACTGW010000328         2664 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.333, whole genome shotgun sequence.
ACCESSION   DACTGW010000328 DACTGW010000000
VERSION     DACTGW010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.333"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            21..767
                     /locus_tag="I9573_003527"
     CDS             21..767
                     /locus_tag="I9573_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4798112.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            767..>2664
                     /locus_tag="I9573_003528"
     CDS             767..>2664
                     /locus_tag="I9573_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4798113.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTYFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLIYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIR"
BASE COUNT         1007 a          286 c          446 g          925 t
ORIGIN      
        1 ttaggaagga tagttaaata atgaaaattt taactgtaaa taatttaagt aaagtatatg
       61 gtaaaaaaat aattttcaac gcattaaatg acataaattt tagtatagaa gatggagaat
      121 ttgtaggaat tatgggtcca tctggtagtg gaaaaactac tcttttaaat atgatttcta
      181 ctattgacaa gccaactaca ggaactatgg aattaaaggg aaagaatcct ttattgttaa
      241 gaggtgagga acttgcatta tttagaagaa gagagttagg ttttgtattt caggatttta
      301 atttattgga tacattaact ataggagaaa atatagtatt gccattgaca ttagataagg
      361 tcagtgtaaa agagcaagat gagagattga atgaagtatc tacaatatta ggtataaaag
      421 atttattagg aaaaagaact tttgaagtat caggtggtca agctcagagg actgctattg
      481 ctagagcatt gattaacaat ccatcaatac ttttagcaga tgaaccaaca ggtaatttag
      541 attcaaagtc atcaaaggtt gtaatggagc tatttcaaaa gataaataaa gaaaataaag
      601 ttactactat gatggtaact catgacccat tagcagcgag ctactgtagt agaatcttat
      661 tcataaaaga tgggtctata tataatgaaa tatataaggg tagtagtaga gagcagtttt
      721 atcaagagat aatggatgta cttacattat tgggaggcga taactaatga atataaaaca
      781 gtttgctatt aacaatattt caagaaatat taaagcgtat ttaggatact tagcaagtat
      841 tgttatatca tcttcactgc tattttcttt tattatgttt acaagtcatc cagatttaga
      901 tgtatctcaa tttcctgatt atttaaaaga aggtctcaag atgagtaaga taattgcata
      961 tctattctta ttcttctttg tgttttattc agttagtgtg tttcttaaaa gtagatataa
     1021 agaatttgga atactttata ttacaggtat atcaaaacgc caagtaatga aattaatctt
     1081 tatagaaaat atgataataa atatagtatc ttctgtaatt ggaataataa taggcttaat
     1141 ctttgcaaag atatttttag tggttatgtc tactttttta gaattatcgc cattaaaatt
     1201 ctatatacca tttaatgcga tattgtctac tttaatctat tttatgattt tagctgtatt
     1261 aacatcaata tttacttatt ttatagttaa agaaaatcag gtgttgaagt tacttaaagg
     1321 tacaaaaact ccaaaaccag agcctaaaac atcactaatg cttgctatat taagtatttg
     1381 tttgtttata atagcgtatt ataatgctat tacctcaacc gaccaatata caacatatct
     1441 gcgcttagtt ccagttacat cccttactat agtagcaact tacttgttct tttctcaatt
     1501 tagtgtattt tttatgaaga agctaaaact aaacaagaga ttttatagaa aaaatacaaa
     1561 tatgctttgg atttcgaact tgatttataa ggtaaaggat aatgctagaa tatttttctt
     1621 gataactata acttctgcag tagcatttac tgctataggt acagtttatt ccttttggaa
     1681 agatgtagag cgtcaaatta atttaatata tcctaacaca atatattatt caacaatgac
     1741 attgcataat gaccctaaaa agcctgatag caaagaaaaa gacaaggaaa gaatgatgtt
     1801 tatagaaaat aatctaaaaa aagagaatgt agactataca aggactaatg gtgaatttaa
     1861 aactgtattt cccaagaaaa gtgattttaa tgtcagaatt atgaaagaat ctaagtactt
     1921 agaaattaca gaaactatag gggttaagcc tataagtttt gaagataatg aatgcatatc
     1981 attattatca tcaagtttac caggaaataa taacgtcaaa gaaaatatta ttgtagggaa
     2041 tagaggttta aaggtggcaa aacaagtgga agaatgtgtt atgccagcat attacaaaaa
     2101 tatgtatgtg gtaaaggata atttttatga tagtatagaa tcaaaattta tagtggatag
     2161 atttactgca tttgaagtag aggattcaag taaaactata gagatatgca gagaatttga
     2221 aaataaattt aataatgagt cagggatgca accatatttg tttttctcta aagaattaat
     2281 gataggaact gggaaattaa tatattctac atttatattt ttagcaatat ttataggttt
     2341 aattttcttt gttactacaa gtagtttttt atataataaa ttttatatgg attgccaagt
     2401 tgataagaaa aagtatgagc aattaaataa attggggatg acatataatg agattaaaaa
     2461 agcttcaaca attgagattg gaatagtatt tttgtttcct tatgtagtgg ctgttataca
     2521 ttcaatattt gcccttactg cactgaaaaa ttcttttgat atagaagtag catcatcagc
     2581 tgttttggta atgggaagct tgttcttggt tcaaatagtt tattttttaa ttgtaagaaa
     2641 taattattta aaagagataa ggtt
//
LOCUS       DACTGW010000329         2639 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.334, whole genome shotgun sequence.
ACCESSION   DACTGW010000329 DACTGW010000000
VERSION     DACTGW010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.334"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            272..1462
                     /locus_tag="I9573_003529"
     CDS             272..1462
                     /locus_tag="I9573_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008429242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAT4798114.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1632..2594
                     /locus_tag="I9573_003530"
     CDS             1632..2594
                     /locus_tag="I9573_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAT4798115.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHIFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVLSPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
BASE COUNT          959 a          315 c          371 g          994 t
ORIGIN      
        1 actatatcaa ctatattata ttaaacaatt aatatagtca agaatatttt attctctaaa
       61 tctatgtttt attaattctt taaaaaacta ttatataaaa taatcatgat aaaattagaa
      121 attggaattt ttaatatgca ctgtacataa atatatttta tataaaagtt tatattttca
      181 aacaagtaat ttttcgaaaa ttgtctaaat tcagtaaaaa tatggtaata tatacataag
      241 caatataaat ctataaaaag gagtgttaca tatgatatct aataaaatgc aaactttagt
      301 tgcaaatagt tctgttataa gagctatgtt tgaagaagga aagaaacttt ctgatattta
      361 tggtgaggaa aatgtttttg attttagtat aggaaatcca agtgttgaac ctccagagac
      421 tattaaagca gttataaatg atattttaaa tgaagaatct ccaaatttag ttcatggata
      481 tatgaacaac tcaggatatg aggatgtaag agatgctatt gctgaacata taaataaaaa
      541 agatggatta aatcttacaa gagaaaattt aattatgact tgtggtgctg ctggtggtct
      601 aaatattatt ttaaaaactc ttttaaatcc tggtgatgaa gttattgctt ttgcaccgta
      661 ttttggtgag tataaaaatt atacagaaaa ctatgatgga aaattaatag aagttcctac
      721 aaatatagag acttttgaac cagatttaga tgctctaaaa aatgctatta ctccaaaaac
      781 aagagcttta ataataaaca ctccaaataa tcctactggt gtcatatatt ctgaagaatt
      841 attaaaaaat cttggagaat tattggattc aaaacaaaaa gaatttaata caagcatata
      901 tctaatttct gatgagccat atagagaaat aatttatgat ggtgccaaag ttccttgtgt
      961 actaaaatac tatagaaatt catttatagg gtactcttat agtaaatcat tatctctacc
     1021 tggtgaacgt ataggatata ttgttgctaa tgggcaaatg gatgattttg atgatgttat
     1081 gtcttcatta aatgttgcaa atagaatact tggatttgta aatgctcctt ctctttttca
     1141 aagggttatt gcaagaagtt tagatgcaga agttgatgta aatatttata aaaagaattt
     1201 tgaccttcta tataatagtt taatcgatat gggatattct tgtgttaaac ctaatggtac
     1261 attctatttg ttcccaaaag ctccaattga agatgataaa aaattctgca atgatgcaaa
     1321 acaatttaat ttattattag taccaggttc ttcttttggt tgtccaggtc attttagagt
     1381 atcatattgt gtatcttatg ataaagttaa aagttcttta ccagcatttg aaaaattagc
     1441 caaattatat aatttaaaat aactaaatca aagtaattca aataattaac taccttagta
     1501 tagaatactt acattctata ttaaggtagt ttataatttt atgtactata ttttatacat
     1561 accatattat tccaatatgc ttgaatattc ttatctatag tactaatata aattacaagg
     1621 aggtgtttga cttgaaaaaa ggttatgttt atataatact gactacctta ttttttagtt
     1681 caatggaaat tagtctaaaa actgtcacta atgactttaa tcccttacaa ataactttat
     1741 caagattttt tgttggcggt ttagtcttac taccttttgc tgtaaaaaga ttaagagctc
     1801 ttagtctttc tattacaaaa tcagacttaa aatactttgc atttttaggt tttatgtgtg
     1861 tagtagtaag tatgtcattg tatcaactgg ctgttctaaa cacaaaggca tctgtagttg
     1921 ctgttttgtt cagttgtaat ccagtctttg taatgctttt agcttactta attttgaagg
     1981 aaaaaatata taggcacaat gtaatttcac ttatattaga agttcttgga attatagtta
     2041 taataaatcc attgcataca aaactaactc ttagtggaat attcttaaca ttatcttctg
     2101 ctattatttt ttcaatgtat actgtatttg gtaaaagaaa aaccttaaaa tttggtggaa
     2161 tagttgtaac ttgctttagc tttatatttg gtagtttgga aatgttgata ttggttttac
     2221 tcactaaaat taattttatc gcagatatat taaataaaaa taaacttcat atatttgcaa
     2281 atatccctat ttttagtgga tataccttac acaatatgcc cattatgatt tatgtattcg
     2341 tatttgttac aggtgttggt tatgcacttt actttatggc aatggaagct acatctacta
     2401 gtcttacatc tttagtattc ttctttaaac cagtactatc tccaatacta gcattattaa
     2461 tacttaaaga aattatccct ataaacatgg ttataggaat tttattgata gtaattggtt
     2521 caataatttc tattatacct acgataatta ctcaaatgca taataaacac gttgaagcct
     2581 tgtataagga gtagtaattg ataatctatc aagttataca taagacaaaa tttattaga
//
LOCUS       DACTGW010000330         2627 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.335, whole genome shotgun sequence.
ACCESSION   DACTGW010000330 DACTGW010000000
VERSION     DACTGW010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.335"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            80..784
                     /locus_tag="I9573_003531"
     CDS             80..784
                     /locus_tag="I9573_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4798116.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            786..2273
                     /locus_tag="I9573_003532"
     CDS             786..2273
                     /locus_tag="I9573_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4798117.1"
                     /translation="MNNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVFSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
BASE COUNT         1068 a          230 c          476 g          853 t
ORIGIN      
        1 tatctattgg gatttaatta tttaatcaat atatttatac catttaattt aaataaaaag
       61 tctattaagg agtgagctta tgagtaagtt aatttatata gcagatgatg aagataatat
      121 aagaaatttg gtaaagacat tcttaaaaaa tgaaggtcat gatgttatgg attttaaaac
      181 gggtgatgaa cttttagaac aatttaatat aaaggagtgt gacttagtaa tactagatat
      241 aatgatgcct ggctcaagtg gttttgaagt atgtacaaaa cttcgtgaga aaagtacagt
      301 cccaataatc atgcttactg caagagatac agatatagat tatattacag ggataacctt
      361 aggtagtgat gattatttta caaaaccatt tagcccaatg tctttggtta tgagagtaaa
      421 atctatattt agaaggatag agtttgaaaa gaaacaaaat tatgataaat atagtaactc
      481 gatagatatg gaattaaaat ttggtgatgt aataataaat aaaaagaata aaatcgttac
      541 aagtaaaaat gtaaatatag accttacacc aaatgaatat aatctattga cttatttgtt
      601 tgaaaatata gatagagcag tttccagaga tgaattatta aacaaaatat ggggatacga
      661 tatagaagtt gagacaaggg ctgctgatga tacagtgaag agattacgta aaaaaatttt
      721 agatacaaat attttaatag aaactgtatg ggggtttgga tttaggttga aggagaaaag
      781 ttaagatgaa taatggaaat gacaagaata gaattggaaa gattaaatcc aaatttaaat
      841 taaagttctc aaagaaaaat ataagcataa aaactaggat tttgttgagc atactttgtt
      901 tgataattat agtatttatg ataatattag tatcttttaa tgtatttctt gatacatata
      961 taaaaacaac tgcgaatgaa gaattgacga agtctacaga aatagtagaa catatggata
     1021 gtaattttag acctattaag ccaccacaag atgatggaaa gcttcctccc aaagtattat
     1081 ctaattttat gagaaatgta caagataaag ttaggatggc agaaacacaa tcagatgctg
     1141 atgctatggt agttgattct aagtataacc tgatatttcc aacaagagag gatgatttct
     1201 taaaaaatgt tgatgagatg gagctgatta gggagtgtat tcaaaatgaa aaattagatt
     1261 taaattcaga tgaaaataca cggatatcta ccagaaataa ggattattat atttcaactg
     1321 taaaaattac tagtatatat ggtgaacaag acaaatatct tatacttttt atagacatat
     1381 caaaaactct aaatctagca caaaagataa atattgtatt aatatctgta atgtgttttg
     1441 ctggaatatt agcaatattt acagctgtga ttttatctga gaaaattgct aaaccgataa
     1501 aagagttgtg tgagtttgca aaaaaaatgg gtcaaggaga tttcaaaaga acctactttg
     1561 acttttcaga taaagaatta gtagagctct caactgttat gaataagagt gctgagtatc
     1621 tggataagta tgataatgaa caaaaaatat tttttcaaaa tgcatctcat gaattaagga
     1681 caccactcat gtctattaaa ggttatgcag aagctataaa atataatgtt atagatagta
     1741 aacatgcaag tgatataatt cttgaagaaa gtgataggtt aagtgatatg gttgaagatt
     1801 tattatacat ttctaaaata gataatataa ctaaagattt tgaacttgta gaatgtgatt
     1861 taagagaagt attttcaaat tgtggagcta ggcaaaatgt aagagctata aataagggga
     1921 taaaatttat ttatgaattt gatgaagaaa tggttttatt tgaatgtgat gaaaaaaaca
     1981 tttccaaagc atttatgaat cttatagaaa atgctttgag atacgctaag agtgaaataa
     2041 aaatagtgtg taagtataat caaaaaaata tagttgtgat aatagaagat gatggaattg
     2101 gaatcaagaa agaagattta cctcatgttt ttgaaagatt ctataaggga gtagggggaa
     2161 atcatggaat tggattatca atagttaaat ctattgtaaa taagcatggt gggcgtatat
     2221 acgtagaaaa tggcaaaaaa ggagctaagt ttactatatt atttaaattg taaaaaaatt
     2281 agagaaaata taaagatgtt tgaagttgtt ttttatatat atctgttgta tactgttgaa
     2341 aaaagaacaa tattaagctg acatatatta caataattga catttaaagt aaatgcttaa
     2401 atatagatac gaaaggaaaa gtaaaatctg aagtgtattt aggtttaagt aagcttctta
     2461 ttttataaaa aatatgtatg aaagaacagc tgatttttaa tattacaaat ttagtaacgg
     2521 ttgtatttga agcttttgtc atccatatgt ttttatcaga ctttttaggt cagaaagaag
     2581 ggtatataaa agttgtaaga tatgctaaac tgggttttat aatatgt
//
LOCUS       DACTGW010000331         2585 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.336, whole genome shotgun sequence.
ACCESSION   DACTGW010000331 DACTGW010000000
VERSION     DACTGW010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.336"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(205..1455)
                     /locus_tag="I9573_003533"
     CDS             complement(205..1455)
                     /locus_tag="I9573_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HAT4798118.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLYEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDSDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1487..2428)
                     /locus_tag="I9573_003534"
     CDS             complement(1487..2428)
                     /locus_tag="I9573_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAT4798119.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          773 a          496 c          243 g         1073 t
ORIGIN      
        1 taaatataca aatacaatta tatgaatttt catcctcaat tatagcatag aatatgacta
       61 tctatatcct aaaattatcc atttctaaaa aaaggtaact attattaaga atatcaactt
      121 atagaattta tattccacat acgattttca atcaaaaaaa caagggctaa aaagtgaatt
      181 gtacactttt tagcccttta ttaattattc atttatttca tgtaagaata gcccacatct
      241 tactttaggt tcaaaccatg ttgacttagc tggcattatc ttatttgcat ctgctactct
      301 tattaaatct tcaatactag taggatacat tgcaaatgct actttcatat cttcattaac
      361 tcttttttct aattcttcaa gacctctaat tccaccgata aaatcaattc ttttatctac
      421 tctaggattg tcaattccta aaatagcatc aataacatag tcttgtaata tagaaatgtc
      481 taatgagtca acagggtctt ctatttctaa taaattttta tttgctttca ttttatacca
      541 cttatcacct aggtacattc cgaaagttcc ttttttgtca ggtttatatt tttcttttcc
      601 ttctatttct tctagttcaa aattttcttt tattttattt ataaattctt cctcgcttaa
      661 accatttaag tctttgataa ccctattata atccaaaacc attaaattct catctggtgc
      721 aatcattgct atgaagtaat taaattcttc tttgccagta taatttggat ttttagctct
      781 catttctttg gctacatttt ctgctgaagc tgtcctatga tgaccatctg caatgtatag
      841 ataatctaat ttttcaaatt tatctactag gttatctatt atatcatcac tatcaatctt
      901 ccatactgta tgctctatct tgtcatctgt tatgaaatca tataaaggtt cattattatc
      961 cataaaatca ttgattatat catctatcac cctttgatgt ttgtaagtta caagtatagt
     1021 tcccgtatta gcatcacagt attttatgtg tttagttcta tcaatttcct tttctggtcg
     1081 agtgtactca tgttttttta ttgttccatt caagctttca tcaactgata tacatgctac
     1141 tataccaact tgagctctac catccatgat ttgtttatat atatatatac actctttgtc
     1201 atcttttact agaatttctt gttttcgaaa ctcatataaa ttttctcgcg cttttaaata
     1261 aactttttca tcatataaat ctatattttc atctaaatca atttctgatt tatctatatg
     1321 caaaaatgaa tatggattcc cttctgcaat tcttctagct tctttactat tcataacatc
     1381 gtaaggaagt gctgctactt tatctactat atatttattt ggtctaatcg ccttaaatgg
     1441 tttaatcgtt gccattacta atttcctcct aattatacaa aatattttat aatgctaccc
     1501 ctattagatt gttgaagtca agaaagaaat cttgaatcac atttactatt tcgtctccta
     1561 ttctttcttg agcttctact gtagatgccc caatatgagg tgtaggagaa actcttggat
     1621 gatttactaa gtctaaattt aatgtaggtt cttgttcata aacatctata gcagctgctg
     1681 ataatttacc ttcatctaat gcttttagta aatctttttc atcaactaaa ccgcctcttg
     1741 cacagtttat taaataagcc cctttcttca tcttatttat ttctttttca gttattactg
     1801 cccccttatt cttatcaaat ggtatgtgta aagataagaa atctgcattt tctaaaactt
     1861 cttccatatc acagaattta taattgttaa aaccttgcgc ctcacccatt atgtcagtat
     1921 atataacatt catacctaat aattctgctc tctttgcaac ttcttttgca attcttccaa
     1981 aacctataag tccaagtgtc tttccattaa tttctgtacc tttatacttc ttcttctccc
     2041 attttccatc tctcatagtt acatttgcag tatttatgaa tcttgcaaga acaaataatt
     2101 gacctattgt aagctctgca acagatatac tgcttgcatt tggagtattc ataactttta
     2161 tgccattagc ttgagcatat ttaacatcta tattatccag acctactcca cctcttacta
     2221 tcaacttcaa ctttttaccc tctaatgctt tatctattac atctttagta actttagtag
     2281 ctgaacgtac tacaagtaca tcaacatctt taagtttatc tcctaaaaca tctttttcat
     2341 aaaactgctc tatcacatta aaatctaact ctctcaattt tctcgctgct tctttctcta
     2401 ttccatcagt aactaatata ttatacataa caaactcctc caatttttat tcacttaatt
     2461 tttccaattt ttatttttta aagtattttc atcaataaaa aactttactt aatttaactt
     2521 tctgtttata taattttcaa aatatttttt tattaaagtc atatcaaatt ttcctattgt
     2581 ttgtt
//
LOCUS       DACTGW010000332         2557 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.337, whole genome shotgun sequence.
ACCESSION   DACTGW010000332 DACTGW010000000
VERSION     DACTGW010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.337"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            28..1800
                     /locus_tag="I9573_003535"
     CDS             28..1800
                     /locus_tag="I9573_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4798120.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
     gene            1822..>2557
                     /locus_tag="I9573_003536"
     CDS             1822..>2557
                     /locus_tag="I9573_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT4798121.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVNKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYP"
BASE COUNT         1048 a          237 c          399 g          873 t
ORIGIN      
        1 caattttatt aagtatggag gtgtattgtg gaaaatggta aatttcaatt ttttaaaggt
       61 gtaagaaatt gttttaatat gatttatcta catgataaaa gatatttatt tatctctatg
      121 tttatggcaa tttttcaggg gattgttcca tctatttctt tgttagtaag tcaaaatatc
      181 attaataata tgcaaacatt ttcgaaaagt gtaaaagaaa tattagtttt gttaattatg
      241 tatttaatca taaatataat tccttcaatt tttcaaagtt tttatggata ttatagaata
      301 aagtttaata aaaattttga aaaacatatt gaaacaatga tgttagagaa atctttaact
      361 ttgagtttaa aagattttga ggatgataat acttatgaca tgataaatag agctcaatct
      421 caaaaaggat ttaatattac tatactatct gaaaacctta tttcaataat aagagaatca
      481 gtatctacac tttctcttat tgttgtatta tcacaatttc aactttggct aataatagtt
      541 gttattgtta ttcctgtatt gaaatcatta tattccatta aattaggaaa ggaacaatat
      601 gatgttagtg taaacagaac ttggaaagaa agaaaggcct ggtacataga ttatttgttg
      661 tcaaaaggtt atgcttacaa agagattatg gtatttagaa ttggttctaa acttattaat
      721 cattataaaa aattaaaaga ggatattata tctcaagata tgaatatagc taaaaagaat
      781 tttattgcta cgctattatg tacagtttta gattcaattg tatctgcagg aattatagga
      841 tttataataa ttcaaggtat tttaaagaag attttaatag gagatgcgac tacatatata
      901 caagcagttc aatctataaa ggataatgtt gaatcaatat tttcgttatt tagttcagta
      961 atacaagatc ttttttatac agatttactg tttaaatttc tagattataa ctgtaaagaa
     1021 gactcaattg aacatataaa aatatttgaa aaaatagggg atataaataa aatagagtta
     1081 ataaatattt catataaata caatcattca tcagagtatt ctttaaaaaa tattaattta
     1141 actttaaata aaaatgatcc tattgctata ctaggaaaaa atggttcagg taaaacgaca
     1201 cttttaaaaa tattattagg tttttatgat gattatgaag gtgacatctt gataaatgga
     1261 aaaaatttaa aggatattga aaaagatgaa tacattagaa aagtaagttg tatgtttcaa
     1321 gattatatta aatatgaggc tacattaaaa gaaaatgtcg ccatgggaga tgtatataag
     1381 agtaacttgg aagaaaaagt taaaagtaaa cttaaagaaa ctaaattgaa accaagtatt
     1441 tatgaggaag atggattaga aactatttta ggaacctggt ttggtaagaa gcaactttct
     1501 atgggtgaat ggcaaaaagt agcaatatct agaactctta tgaaagatgc tgatatgtac
     1561 atttttgatg aaccagattc atctttagat tctgaagcgg gaaatgaact cctttcttta
     1621 tataaaaaaa catttgaaaa taagattgga atatttataa cgcaccatat ccaacatgca
     1681 aaaaagataa ctaacaatat tgtagttctt gaaaatggaa agttgttaga aagtggaaga
     1741 catgatgaac ttatggacaa aaagggtgtt tattttaatt tattttgtaa ctgcaaataa
     1801 atattaaagg gaggttaatt aatgaatcaa aaaccattta tacacacatt taaagctgga
     1861 aaatcatatt ttatttatga tgttaatacg gataagattt taaaggtaaa tgctgctgtt
     1921 tataattatc taaataaaat tgagcataat ttagaaaaag aaagtgactg gaacatagaa
     1981 atagaagacg aaattaatac cttgattaat tatggatttc taaaaaataa aagggtaaat
     2041 aaaacaatgc accctgaaac taagtatata aaatatcata tagaaaataa tctatcctct
     2101 attattttac aagtaaccca aaattgtaat ttacgttgtg actattgcat ttattctggt
     2161 tcatacagca acagagttca tacaaataaa agaatgaata tagatgtagc aaaaagaagt
     2221 attgactatt tgattaagaa ttctagtcaa ttaaatgaag ttagtgttgg cttttatgga
     2281 ggagaaccct tacttgagtt tgatttgatt aagcagatag tagaatatgt aaaagtagtt
     2341 ggagaaggaa aggaaatata ctttaatcta actacaaatg caactttatt taatgaggaa
     2401 ataatagaat tctttgtaaa aaataatata acaactatga ttagtttaga tggaggaaaa
     2461 gaaattcatg ataaaagtag aaaatttgca aatacaggag aaggatctca tgatgttgta
     2521 ttaaaaaatg tacgttacat aaaaaataaa tatccag
//
LOCUS       DACTGW010000333         2555 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.338, whole genome shotgun sequence.
ACCESSION   DACTGW010000333 DACTGW010000000
VERSION     DACTGW010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.338"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            16..2361
                     /gene="secA"
                     /locus_tag="I9573_003537"
     CDS             16..2361
                     /gene="secA"
                     /locus_tag="I9573_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAT4798122.1"
                     /translation="MSVLDAILDKADEQEIKKLNLIVDKIESLEKDMEIKENEQLKEM
                     TNTFRLRLDKGESLDDILPEAFAVVREVSKRVLGMRQYKVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYEFLGLTVGVILSNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEIDSILIDEAR
                     TPLIIAGDGDEDLKLYELANNFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNRPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEVISKAGKLDAITIATNMAGRGTDISLGAGDKEEEEKVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIKEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
BASE COUNT         1062 a          258 c          508 g          727 t
ORIGIN      
        1 aggaggtcaa aaaatatgtc agttttagat gcaatcttag ataaagcgga tgaacaagaa
       61 attaaaaaat taaatttaat agtagataaa atagaatctt tagaaaaaga tatggaaatc
      121 aaagagaatg aacaattaaa agaaatgaca aatactttta gacttagatt agacaaaggt
      181 gaaagtttgg atgatatact tccagaagca tttgctgttg ttagagaggt ctcaaaaaga
      241 gttttaggaa tgcgtcaata taaagttcag ttaattggag gtatagtaat tcatcaagga
      301 aagatagctg agatgaagac tggtgagggg aaaactttag ttgaggtagc tccagtatat
      361 ctaaatgctt taacaggaaa aggtgtacat gtaattacag taaatgatta tttggcagaa
      421 cgtgataaag aacttatgcg tccagtatat gaatttttag gattgactgt aggtgtaatt
      481 ttatctaacc aagacccaag tataagaaaa gaacaatata aatgtgatat aacttatgga
      541 acaaatagtg aatttggatt tgactattta agagataata tggtgccaga tttgtctcat
      601 aaagtacaaa gggaactaaa ctttgccata gtagatgaga tagactcaat attaatagat
      661 gaagctagaa ctccacttat tattgcagga gatggtgatg aagatttaaa actttatgag
      721 ttagcaaata actttgtaaa aactgttaaa gaagaagact ttgagatgga tagaaaagat
      781 aaaactatag cattaacagc aagtggtata agcaaagctg agtcattttt tggtataaca
      841 aaccttactg atataaagaa tatagaatta tatcatcata taaatcaagc tttaagaggt
      901 cataagctta tggaaaaaga tgttgactat gttatctcaa atggagaagt aatgatagtt
      961 gacgaattta caggaagagt aatggatggt agaagatata cagatggact tcaccaagct
     1021 atagaagcca aagaaggtgt tgagataaag aatgaatcta aaactatggc tactgtgact
     1081 tatcaaaatt tcttcagact atatgaaaaa ctttctggta tgactggtac tgcaaagaca
     1141 gaagaagggg aatttgagtc aatctataaa ttaaatgttg tccaaatacc aactaataga
     1201 ccagttatta gagctgattt gcatgataag gtatttaaaa cagaagaaga aaagtatagt
     1261 gctgttgtag aagaaataat aaggatacat aagactagac agccaatact cgtgggaaca
     1321 gtttctgttg aaaaatctga gaagttatct aaaatgctta agaagcaagg tattaagcat
     1381 caagtcttaa atgctaaaca acatgataaa gaggcagagg taatttctaa agctggtaaa
     1441 ttagatgcta taacgattgc tacaaatatg gcaggtagag gtacggatat ttctctaggt
     1501 gcaggagata aagaagaaga agaaaaagtt aaaaatttag gtggtcttta tgttatagga
     1561 acagaaagac acgaatcaag aagaattgac aatcagctta gagggcgttc tggtcgtcaa
     1621 ggagacccag gtacatcaag attttttgta agtcttgaag atgatgtaat aaagctttat
     1681 ggtggaaaaa ctatagagaa acttatgaag agaacaagtt caaaggaaaa tacggctatt
     1741 gaaagtaaag cacttacaag ggctatagaa agagcacaaa aaggtgtaga aggtaagaat
     1801 tttgaaataa gaaaaaatgt tcttaaatat gatgatacta ttaatgagca aagaaaggtt
     1861 atatataatg aaagaaataa agtgttaaat gatgaagata taaaagaaga tattcaaaaa
     1921 atggttaaag acatcataca agaagcagga gaaacttatt taattggaag aaaaagagat
     1981 tattatggat attttaaaca tttatatagt acatttatgc ctgcagatac attactaata
     2041 cccggtgtag ataaaaaaag tgtccaagag ataattgata gcacatatga aatttcaaaa
     2101 agggtttatg acttgaaaaa gatgatgatt ggtattgata aagttgcgga gttagaaaaa
     2161 acagtacttt taaaagtggt tgatcaatac tggatagatc atatagatgc tatggaacag
     2221 ttaagacagt atataggtct taaatcttat gctcaaaaag atccgtttaa agaatatgct
     2281 ttagaaggat atgatatgtt tgaagcttta aataaaaata taagggaagc aacagtacaa
     2341 tacttatata aatttaacta aaaaagttca tgtaattttt attaaatgaa aaataaaata
     2401 ttttgattct ttatattgta attacaatta aataattaag gaatacaaat gaaaaagaag
     2461 ctgaaaatca gcttcttttt ctgtgtgaga aatacggaaa aaagagatgc ttgaaatttc
     2521 tttcttccat ttaatattat actataaaga agatg
//
LOCUS       DACTGW010000334         2547 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.339, whole genome shotgun sequence.
ACCESSION   DACTGW010000334 DACTGW010000000
VERSION     DACTGW010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.339"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(60..704)
                     /locus_tag="I9573_003538"
     CDS             complement(60..704)
                     /locus_tag="I9573_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAT4798123.1"
                     /translation="MNNLDKNNVNEVLDMLLRTRRSIRSFTKEVPSKGLIEEIIETGR
                     LAPYAILSVAKQTDFRHFFVISNNSLQMNKIKTILVDVIKTQISVNEKKAKNNITLQS
                     FITSLKMQVEKGVLGIGTAPYLIIVAERRGIPPIEQKSLSHVMQNMWLKSTALGLGFE
                     LVSVIGRLSDNKDFCDILGVPTGEYGFDACAIGFPTTSVTREGRAIPHPSIKWI"
     gene            968..1582
                     /locus_tag="I9573_003539"
     CDS             968..1582
                     /locus_tag="I9573_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAT4798124.1"
                     /translation="MKKVSIDRRTIRTKKMIRSALAELIDEKGFNNISVTDLTQRADI
                     NRGTFYLHYVDKYDLLERIENEIIQEIEEETKHLDSSNMMEIDTSSEPLPFMVKLFEY
                     FRKNSVIIKSILGPKGDPTFERKIKKFIETNLFEKQKLINFNIDEAIISEEYFIQYIL
                     SADLGVIQYWLEKNMKESPEEMALILARMSLLGPLRAVGIRKDS"
     gene            complement(2173..2547)
                     /locus_tag="I9573_003540"
                     /pseudo
     CDS             complement(2173..2547)
                     /locus_tag="I9573_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599264.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          928 a          329 c          351 g          939 t
ORIGIN      
        1 acttattatt aaaaacatgt aagaatagtt tataaatatt ttattttcga tgttgtttat
       61 tatatccatt ttatcgatgg atgaggaatg gctctgcctt ccctagttac agatgtagta
      121 gggaaaccaa ttgcacaggc atcaaatcca tattcacctg ttggtactcc taaaatatca
      181 caaaaatctt tattatcgct caatcttcct attacactaa caagttcaaa acctaatcct
      241 aaagcagttg atttaagcca catattctgc atcacatgag ataatgactt ttgttctata
      301 ggtggtattc cacgacgttc tgccacaata ataagataag gtgctgttcc tataccaaga
      361 accccttttt caacttgcat ttttaaggaa gtaatgaagg attgaagggt aatgttgttc
      421 tttgccttct tctcattgac tgatatttga gttttaataa catcaacaag aatggttttt
      481 attttattca tttgtaaact gttatttgaa ataacaaaga aatgtctgaa atctgtttgt
      541 tttgctactg aaagtattgc ataaggtgca agtcttccag tttcaattat ttcttcaatc
      601 agcccttttg aaggtacttc ttttgtaaaa cttcttatgg atcttctagt tcttaatagc
      661 atatccaaaa cttcattaac attgttttta tctaaattgt tcatttttat cctccactaa
      721 tagtattctt aagatgttct ataatgcatc catataaagg cgaacttttc ggtcatatta
      781 aacatttcct tcaaattaag tcgctatttc tatgaacaca atgttgatta ataatctata
      841 tgtatattat aatatttata ttgcattatg caataaacaa taatatttaa actgttaagt
      901 aataaacatt tactataaaa ttgttaatta aaattaaaaa aatttatatg gaatgaggaa
      961 ataattcatg aaaaaagtat ctattgatag aagaactata cgtactaaaa aaatgattag
     1021 gtctgcatta gcagagttaa tagatgagaa aggatttaac aatatatcag ttactgattt
     1081 aactcaaagg gcagatataa atagaggaac cttctatctg cattatgttg ataagtatga
     1141 cttgttagag aggattgaaa atgaaataat acaggaaatt gaagaggaga ccaaacatct
     1201 tgattcatcc aacatgatgg agatagatac ctcaagtgaa cctcttccat ttatggtaaa
     1261 gctttttgaa tattttagaa aaaattcagt aattattaaa tctatcttgg gtcccaaagg
     1321 tgaccctact tttgaaagaa aaataaaaaa atttattgaa accaatttgt ttgagaaaca
     1381 aaaactaata aattttaaca tagatgaggc aataatttca gaagaatatt ttatacaata
     1441 tatactttca gcagacttag gtgtaattca atattggctt gaaaaaaata tgaaagagtc
     1501 ccctgaagaa atggcactga tacttgctag aatgtcgtta ctcggtccgt taagagcagt
     1561 gggaattcgt aaagattcat aaaaatagtt ttatatatat tagactttga aaatatatgt
     1621 ttaaaaacca ttttttacta ctactgttta taaattaggg gagatatctt aaaatattag
     1681 taaaaggagt gctaagtgag tgtaataact atgagataag aggtttagtt gatatagtca
     1741 aaacttcaaa agaatcaatt agaaaagtta aaatattgca attaaattta aaggagtaat
     1801 atatgaaaaa tgaaaatata agttatttgg cgttaggatt atgttttggt gttgttttgg
     1861 gttcattatt taaaaactta gctattggtt tatcctttgg attagttatt ggagtagtat
     1921 tagacagtaa gaaaaataaa taaaacttca atagtaacat ataacaatta tactattttt
     1981 gtataaaatc attggaaatc aacaatagaa aatatttgtt atttaaaaac taaaaataca
     2041 gtttaaacga cctaaaaata tattatataa ttattataac aagataagtg agagtaaggt
     2101 tatgataata gagtaatatt tttcaattat tactctattt atatttattt agttatacta
     2161 tagtttatat tgttatttta ttttcatatg cagtataggg aatggattac cttgttcatc
     2221 gaactcagat tttttaaaga ctttaaatcc catatgttta taaaagccta atgcttgttg
     2281 attttgctca ttcacatcta cataatttac atttaatact tctattgcat attctacaag
     2341 tctttttcca attctatttc ctatactata attacttaca aataacattt caattttaaa
     2401 atcatgtatt cccataaaag cttttataat accttttttt gtctcttaca cataaaaaat
     2461 tactaacata tttagcacat tctttaactt gaggctttat tgatattata ttttcttcac
     2521 ttaaaaagtc atgtgttgct cttactg
//
LOCUS       DACTGW010000335         2496 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.342, whole genome shotgun sequence.
ACCESSION   DACTGW010000335 DACTGW010000000
VERSION     DACTGW010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.342"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            5..460
                     /locus_tag="I9573_003541"
     CDS             5..460
                     /locus_tag="I9573_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4798125.1"
                     /translation="MRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQFIEYNS
                     IIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLMTTGIQY
                     CICNKFNLTEITVNSSPYAVEVYHKLGFEDIAVEQIVDGIRFTPMKLLI"
     gene            complement(829..1386)
                     /locus_tag="I9573_003542"
     CDS             complement(829..1386)
                     /locus_tag="I9573_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAT4798126.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
     gene            1644..2342
                     /locus_tag="I9573_003543"
     CDS             1644..2342
                     /locus_tag="I9573_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT4798127.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHEYQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTILENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          925 a          319 c          306 g          946 t
ORIGIN      
        1 cttaataaga gaattaaaag aagatgatat taaaaatgca ttagacttaa tctggaatgt
       61 atttgaagaa tttgaatctc cagattattg tgatgaagga attacgacat ttcatcaatt
      121 tatagaatat aattctatta ttgaaaaaat caaagaaaaa acactcttct tttggggttg
      181 ttttgactct aaaaaactta ttggagtctt agctattaga aatttaaatc atatatgtat
      241 gttatttgta gataaaaact atcataaaca tggtgttgca aaaaaattaa tgactacagg
      301 catacaatac tgtatttgta ataaatttaa tttaactgaa ataactgtaa attcatcacc
      361 ttatgctgtt gaagtttatc ataaattagg atttgaagat attgctgttg agcaaattgt
      421 tgacggtata cgttttacac ctatgaaatt gttaatataa taaattaaat aaaaagcaaa
      481 gtctcttaaa tattttatag ttactttgct tttaaaccaa gatttaaaat aatactatct
      541 tttttgtttt ttaataatat actttaaaag ccttactaat atgaactata atttattttt
      601 tgatatcctt tttgattaac atataaatta attcaattat taaacataaa ctatgttgtt
      661 tctctttata tctcttcttt gtaaacaata ttgggcattt gaaaataaat attcttaaaa
      721 tatactgttc tcctgttgat tttcctttgg tacaaatgat aaaattttaa tttacatgct
      781 ccgtaatgaa gtgtttaata aataagttaa aataatttat ttacctcctt aatcatattt
      841 tttatgaata cctaataaca cagtatcaat agaatcggct aaacctttta tagcttcttc
      901 tttactcaaa agtgatttcc caagttgcaa agtcgaataa ccatgaagta cactcattat
      961 taagtttaat acttcacctg tattctttgt atttagatta caagaaagaa taagtttttc
     1021 taataaggat ttatattcat caaagatttc taatgtttca ttatttccat gccaagtagc
     1081 ccactgaatt gtttcataaa caccaggatt agaaattata aaattaaagt atgctatact
     1141 tatacacttt atagctgtat ctccaaaatt tccaatagtc gcctttatca tttgactatt
     1201 catctctctc attcctttat gagctacttc aagtaataaa tcatctagac tttctatatg
     1261 attgtacaaa gatggtgtac gtattcctag cttttctgca ataactttta aagaaacttt
     1321 gttaagccca tctttattaa ctatatctga tgtagcttgt ataactattt ctctactaac
     1381 acgcatgatt ttccctcttt catttaaaat taattacttt attagtttag ctaatctaat
     1441 tagtttcgtc aattattgta atttataaaa ttctttatac tcttataaaa tcttgacagt
     1501 ttaattataa tttaatataa tactaattga attagtttta atactaatat aattagtatt
     1561 atattaaatt atatattaca acattatttt atagtttgat taattcaaca atcactaata
     1621 tatcaaactg aaaggagatt tttatggaaa aaaaatattt cgaaattcat ggagttcctt
     1681 caatcctttg gggaaatcct tcaagtaaat tatatattta catacatgga caatgcggca
     1741 ataaagagga ggctgaagct tttgctaata ttgcagttcc acatgagtat caagttttaa
     1801 gtgttgattt gcctgaacat ggagaacgta aaagagaaaa aaattcattt aacccatggc
     1861 atgttgttcc agaattagta tctattatgg aatatgcaaa gtgccactgg aatcaaattt
     1921 cgttatatgc aaatagcatt ggagcatggt ttagtatgtt aagctttgaa aaagagaact
     1981 taagagagtg tatttttgta tctccaattc tagatatgca acagctcatt tcaaatatga
     2041 tgctttgggc aaatgtatca gaagaacagc ttaaacatga actaattatc ccaacttcat
     2101 ttgggcatac tctttcatgg gaatatctac aatatgtaaa acaacatcct atcactcaat
     2161 ggaatgtacc aacaaaaata ttatatggta aacatgatga acttactgaa atcggtgttg
     2221 tagagaaatt tgtagaaaag tttaattgca accttactat tctggaaaat ggcaaacatt
     2281 ggtttaacac accacaagaa ttaaattcat tatatgaatg gttaaatact ttagttaaat
     2341 aaaaaataat tatattatta atatgattaa attatttata aatgttattt aattaatttt
     2401 attttagcat tattaaaata actacataaa gaataaatta gttattatta aagaaattaa
     2461 aagtatatag agtattaata attttttaat aattag
//
LOCUS       DACTGW010000336         2465 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.343, whole genome shotgun sequence.
ACCESSION   DACTGW010000336 DACTGW010000000
VERSION     DACTGW010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.343"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(147..446)
                     /locus_tag="I9573_003544"
     CDS             complement(147..446)
                     /locus_tag="I9573_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798128.1"
                     /translation="MGFYINYVVCKVKILFSSFSKIILFYINYVVCKLFITLKAILLK
                     TQIIVTTHSPHILQIDSKEEMIVLDMAESGNVYKKELELGEYGVLGWTNEGLYLH"
     gene            668..850
                     /locus_tag="I9573_003545"
     CDS             668..850
                     /locus_tag="I9573_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAT4798129.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            921..1766
                     /locus_tag="I9573_003546"
     CDS             921..1766
                     /locus_tag="I9573_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798130.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINNSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
BASE COUNT          999 a          310 c          261 g          895 t
ORIGIN      
        1 aaagactctt tttagagtct ttttttattg ttagtatatg taagataatc attggtgaag
       61 taaataaggt atattgagta atgttcttga tagtttatat gctaatgaat ttcaaaaaaa
      121 taagcactcc tataaaaaga gtgctcttaa tgtaaatata agccttcatt ggtccagcct
      181 aataccccgt attctccaag ttctaactct tttttatata cattaccact ttcagccata
      241 tctaacacaa tcatttcttc tttagaatca atttgtaaaa tatgaggact gtgtgttgtg
      301 acaattattt gtgtttttag gagaattgct tttaatgtaa taaaaagttt acatacaaca
      361 tagttaatat aaaacaaaat tatttttgag aatgagctaa ataaaatctt aactttacat
      421 accacatagt tgatataaaa ccccaaaata aacttagcat ctccaatgtt tacacatata
      481 caatgctctt aaaattgcag tgaacatgca atagtgcaat tgataatact tatcatatat
      541 tctcaatacc ttatatttca actgctaaac ctcaatttgt cacaaatatc gctcactgca
      601 aaatttctat atttttatta tatcatagca tataattaat attttcaatt aatttttaca
      661 tttaattatg tttaaattct ataataatgc aactttaatg tttctttctg gaactaatat
      721 aaaaactatt tcagaaagat taggtcatgc agatataaaa ataactatga atagctactc
      781 tcagttttta gaagagatgg acagagaagc atttgaaaat ttgagcaaaa tactatttat
      841 tgtattttaa agaagatgac ttattcaata tttgatgcta gaatataatt aataaaaatt
      901 taattatagg tgattgtatt atgaacatta ttaaaagcat aactttactc ttaacattat
      961 taattgtgat attaatgaca ggatgttccc caagagaaag tgctagtgaa gttgcaaaaa
     1021 aatatattga taagatgaac tctggtgact atgaaacagc atatagttta ttaagtaaag
     1081 atagtaaaaa aaaaattagt atagaaaatc ttgaagaatt tcaaaatatt ttagttagta
     1141 caaaaagaat aattagttat aaaataggta aagaaaaaat cataaaaaaa tataaacatg
     1201 atggaaaaga atataaaaat gttgttaaat tagaagaaac ttttaatata aaaaaccttc
     1261 tttataaaaa agatgattct ctaaaagtaa atagatattt tgtagttgaa aataacaaat
     1321 acaagctatt attgtatgaa agtttcaaaa aagaatttgg tattaatagt atggaccttg
     1381 ctcaacttaa gcttaatgat tttggactaa aatactttaa agaagttgac cttatactaa
     1441 aaaaagctat tgctattaat cctactgatt caaagcttta ttatgcccaa gcatgtaatt
     1501 atatgaattt tgatggattt cctggttatt cagatacaac atataatgct ttagattcaa
     1561 ttaataattc tttaaagtat ttagataaaa atgattctga ctacaaaaaa aatgcaagta
     1621 atatatataa cttaaaagga gttattctta tgtttcacaa aagatttgat gaagcacaac
     1681 aatatttaaa taaagcctta tactttaaca aagataatga agacccaaaa tataatctaa
     1741 atacaataaa aaaacatcta aactagatat ttaatttttg aatttataaa atattttttt
     1801 atgtataatt cagcactgaa tcataatctc tactaaacta attacaagtt taaattataa
     1861 agacttattt ctatataaat attactctat aacaataagt ttcttattta aattttaaaa
     1921 tattatctaa aaatcataaa aaagaaggta taaagagcta ccctcaagct caatatacct
     1981 tataaatatc taaacttcat tttttactaa tttttctata tctttgataa tatgttcttt
     2041 agattttttt ccagctatgg catcaaaaat tgcaacagcc tttttctctc caaacttttc
     2101 tgctacatca ttaaagtcta aaaatctaac actttctttt ggtttaccaa acatataaca
     2161 cctctcatac atttttatat atttattata acttctatag acttattttt caccaataaa
     2221 cgttagacaa attagtccct ttattttttt aaactagctt taccttcaaa attcttaaca
     2281 aaaacagttt attctactct ataaataaaa atagacaatt taatttgtag gaaactgata
     2341 atagaataca gtaaataaaa ctttgtttaa tctatctgaa aaagttatct attaaaactg
     2401 ctatgaattt ataaagaata ttctagttca taaaaataca aatatgtaaa tataatcacc
     2461 ttaat
//
LOCUS       DACTGW010000337         2458 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.344, whole genome shotgun sequence.
ACCESSION   DACTGW010000337 DACTGW010000000
VERSION     DACTGW010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.344"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(131..469)
                     /locus_tag="I9573_003547"
     CDS             complement(131..469)
                     /locus_tag="I9573_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAT4798131.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            complement(648..1019)
                     /locus_tag="I9573_003548"
     CDS             complement(648..1019)
                     /locus_tag="I9573_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAT4798132.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            complement(1067..1675)
                     /locus_tag="I9573_003549"
     CDS             complement(1067..1675)
                     /locus_tag="I9573_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HAT4798133.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            complement(1700..>2458)
                     /locus_tag="I9573_003550"
     CDS             complement(1700..>2458)
                     /locus_tag="I9573_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAT4798134.1"
                     /translation="TMIDNQLLSIYKNFHSGKSFNMKVSKKEIEKLFAKLVYDAFSEK
                     MNKSIGFVVDFKKPCETILNYSFNKQDISDEVLGNFMLDEFARLNEIVNKDIDFNEQI
                     EFEREPTFDDKFCKDLGNHFRSRLNTEFPWNLFFRILFEIEPYNNTKDFENFVEFIKE
                     NVLLEENREQLLCQFEEDSKLIIEDIYDCIFNSISEIDSVEILYNYNLEKINFTSSEI
                     INCICREYECYGEYLEEKEKLMNTFKEKIIQIFE"
BASE COUNT          800 a          450 c          184 g         1024 t
ORIGIN      
        1 acttatttat ataatatcac cctaatttta ttgtcataat agttaaaatt tcttctcaaa
       61 ttagtttttt caaataaaaa aaggcatcca taaaattttt aaaagatgcc tctttttaac
      121 tatttatatt ttatttttca agaatactat tatcatcttc taaaacaact gctgttccat
      181 aagcaagcat ttctgctgcc ccttgcataa ctgaagctga agacagtcta aatgctatta
      241 ctgcgtttgc acctttagct tctgcatctt caaccattct acctatggca atttgtcttg
      301 cttcagtgag catttcatta tatccagtaa gttctcctcc aacaagattc ttaaaacttg
      361 ctcctatatc ttttccaaca tgttttgctc ttattgtact tcctctcact aatcctaaaa
      421 cctttgatat ctttttacct tctacttttt ctgtagttac tattaacata atatatcaac
      481 tccttagtat cctatttaat acattatatc atgtaactca caattctttt aacaaatatt
      541 gtaatttaat gtttcattaa ccaatctgta cttttattaa ccaatctata ctataaaata
      601 atatgtttta ttaactaatt atatttttat tattaagaaa tataatttta tctagtcaac
      661 tcatcatata aacttaaatt atattctata tttaatttta aatctttagt ctttttaatc
      721 atcatttcac taaagcttcc tttttcaaac cacatatttt gtatatccaa aagctctact
      781 tcttctaaaa tcacatccat cttagattgt ttatcaataa tagctacaac atcaatattg
      841 tgtggaacat cttttaaaca ttcatacaca tctattcctt caataggttt tttctcttca
      901 tctatagcaa caactttgta catattcctt ttaagttcat ctaatatttt atatgcttta
      961 ctttctttat cgctattatt taatattact gcccaagaat cgcatttaag taaattcata
     1021 aactctcctc catataaatc ctactctatt ttacaaccat agctaattaa actttagtta
     1081 atttttcata ctcatcaaca gtgtttacat ttaaaaataa atcttcttca tcaaagtata
     1141 tataactttt atctatttta tctataaagc ttctgagctt taaatcattt tcccttatac
     1201 ataaagatac tttatttatt atactctttt tatatccaga acacaatggt tgtaactttc
     1261 catttgtcac tgaaatcaca cttttatcat tcattttatt agctattctt tcgactattt
     1321 gattatttag atatggcata tcacatgcaa ttgtaactat atcatacttt gcatattcaa
     1381 gagcagtata tattcctcca attggaccaa catcttttac tatatcatta taaactttaa
     1441 tatcgtattt agaatataat tcttcattat tagatattat tatcaactca tcaaagtttt
     1501 taaatgcttc tattgcaatt tcaataaaaa gcttatcatg taactctaaa aaggctttat
     1561 ctctacccat tctagaattt tttccccctg ccaaaattac acctgttttc tctatacaaa
     1621 tcgcctcctc caaaataatt ttacttaatt catatatatt tgaaaaatgg cgcattagcg
     1681 ccacttcagc attgacctat tactcaaaga tttgaataat cttttcttta aatgtattca
     1741 taagcttttc tttttcttct aagtattcac cataacactc atattctcta catatacaat
     1801 ttatgatttc acttgatgta aagtttattt tttcaagatt atagttatat aaaatttcaa
     1861 cactatcaat ctcacttata ctattaaata tacaatcata tatatcttca attataagct
     1921 tgctatcttc ttcaaattgg cataataatt gctccctatt ttcttctagt agcacatttt
     1981 cttttataaa ctctacaaag ttttcaaagt ctttggtatt gttatatggc tctatttcaa
     2041 ataatattct aaaaaatagg ttccatggaa attctgtgtt taatctactt ctaaaatgat
     2101 ttcctaggtc tttacaaaac ttatcatcaa atgttggttc tctttcaaat tctatttgct
     2161 cattaaaatc tatatccttg tttacaattt catttaatct tgcaaactca tcaagcataa
     2221 aattacctaa aacttcatca cttatatctt gtttattaaa agaatagttc aatatagttt
     2281 cacatggttt tttaaaatct acaacaaaac ctatactttt attcatcttt tcagaaaaag
     2341 cgtcatagac tagtttcgca aataattttt caatttcttt tttacttact ttcatattaa
     2401 aagacttccc tgaatgaaag tttttataaa tacttaaaag ctgattatct atcatagt
//
LOCUS       DACTGW010000338         2457 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.345, whole genome shotgun sequence.
ACCESSION   DACTGW010000338 DACTGW010000000
VERSION     DACTGW010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.345"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(548..1099)
                     /locus_tag="I9573_003551"
     CDS             complement(548..1099)
                     /locus_tag="I9573_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4798135.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1290..2252
                     /locus_tag="I9573_003552"
     CDS             1290..2252
                     /locus_tag="I9573_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAT4798136.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
BASE COUNT          943 a          262 c          410 g          842 t
ORIGIN      
        1 aacttcttaa attatattta gatataggag tgttatgata aaagtaagat tatgtgttgt
       61 ttatattaaa tataaagtgc atatagtgat aaaacaaaag tttcatataa atgaagaaaa
      121 aatataaaat aatttattta actacttaaa aataaactaa aagggataat aaaatagctt
      181 ttaggttatg gtaataaagg tatttaaaaa gagtgcataa gcactccaat tgtgagaaat
      241 acatacacaa atcattatta caacaatgaa aaatgtacat ataagagacg ttttgtattt
      301 gttttaagtg ataatatttt aatactaact aaataattat cacaataata aaaaagaaaa
      361 aggagtattt tgtactccat ttgtcaaaaa tataaaactt ttatataagc ttattataac
      421 ataattgata aaagtgtaaa gctatataat gaatttataa agaaaaataa aataaaaaag
      481 ataaatccta atcaaaggat ttatcttttt gtatagaaaa taattattct attattaaat
      541 ggcatggcta aactggataa taatttatta ttactgcttt caatgttttt tctccttgat
      601 tatggtatgt atgaattgat tcagctgaaa aacaaataga gtcattttct ctaagttgat
      661 aaatagagtc atttacttct aattttaatt gaccttctat aatcataata tattcctgtg
      721 atttttcaga atgaccaaca gatgtataac tatgaccttc ttctaattcc atttgaaata
      781 attcgaagtt tctagttggt gtattgggat aataacaata aagacgataa tctttatttt
      841 ctgaaacttg tacatctata tcagtttttg atacaataaa agtttcattt tgaggttgtt
      901 ccagtattgc agtatatgga acatttagtc cacttgcaat tttccaaatt gtgtttactg
      961 ttgggtttga gtctcccttt tcaatttgcg aaagcattac tttacttacg ccagataatt
     1021 cagcaagttg acctagactt aagttacgtt cattacgtaa tctatttaaa tttttagcaa
     1081 taacagtatt aatactcata gtaactcctt aaaatgtaaa ttattgacaa atataattta
     1141 cgaatgtata atttatttaa cattatttat aatttacaaa tgtataatat aatgaacgtt
     1201 aaatacactg tacatccgta atataaatta tactgcataa agagttgttt ttcaacggat
     1261 gatagttgag agattaaggg ggaattgtaa tgcgtgtact taataatgtt acagaggcta
     1321 taggcaatac tcatatgtta catttatcca aaatagcaga tgaatttgga gttcaaggta
     1381 atatttatgc aaagatggaa tacttaaatc cgggatttag taaaaaggac cgtgtagcat
     1441 tacaaatgat taccgaagca gaaaatgcag gtttattagt tccaggtcaa gcagttgtgg
     1501 aattaacaag tggtaatact ggaacaggac tagcaatagc ttgtgcatgt aaaggatata
     1561 aatttatagc gtgtatgtca aaaggaaatt ctatggaaag agcaagaatg atgaaagctt
     1621 taggtgctga agtagttctt gtagaccaaa tgcctaattc agttcacgga caggtatctg
     1681 gtgaagattt ggcacttgta gaaattgagg ctcaaaaaat agcaaaagaa agaaatgcat
     1741 ttagagcaga ccaatttaat ttagaagctt gtaatcatgc acatgaaaag tatacagcag
     1801 aagaaatgtg ggaacaattg gatggaaata ttgatgtatt tgtagatttt cttggaagtg
     1861 gtagtacttt tgcaggatgt tcaaaaacac ttaaaaaaca taattcttct atcaaatgtt
     1921 atgttgttga acctgaaaca gcagcaattt attcaggtaa agaaataact gaccaagggc
     1981 ataaaataca aggtggagga tattctatgg atttgccatt agtagacaag aaacttattg
     2041 atggttatat tcaagttaca gacgaggagg caactgatgt agcaagaaaa ttagctaaat
     2101 tagaaggtgt atttgcagga ttttcttcag gggcaaatgt gtgtgcagca attaagttgt
     2161 taaagagcag tgaaaaagga aaaaatatag ttttaactct taatgatagt ggtttaaaat
     2221 atttaagtac tgatttatat gaagaattgt aataatagca caatagttat gcaatgtttt
     2281 ttaaattaag tatatagatt taaattataa aaagtatctc aatgtgattt ttgaaagtta
     2341 taaaagagat acttttttat tttgtaaata tggagtatcc tacttataag tattaagcta
     2401 tatagatatt gatgattagc tatgcaataa aaaagagtat gtgtttttgc atttata
//
LOCUS       DACTGW010000339         2419 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.346, whole genome shotgun sequence.
ACCESSION   DACTGW010000339 DACTGW010000000
VERSION     DACTGW010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.346"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..193)
                     /locus_tag="I9573_003553"
     CDS             complement(<1..193)
                     /locus_tag="I9573_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAT4798137.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENIT"
     regulatory      complement(650..747)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            974..1357
                     /locus_tag="I9573_003554"
                     /pseudo
     CDS             974..1357
                     /locus_tag="I9573_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      1713..1806
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1889..2374
                     /locus_tag="I9573_003555"
     CDS             1889..2374
                     /locus_tag="I9573_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAT4798138.1"
                     /translation="MISELMLKNFGKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT         1015 a          270 c          323 g          811 t
ORIGIN      
        1 tagttatatt ttctgcttga ggacctctag cacctttaac tatatcaaag cttacttgtt
       61 gaccttcttc taatgattta aatcctgaag tttgtatagc tgagaaatga gcaaacacat
      121 catctctacc ttccattgat ataaaaccga atcctttttc attattaaac catttcacta
      181 ttccattatt cattaaaaat actcctatta acctatatca agatatactt gatttaattg
      241 tgtattacca caaatactat tgtatcatag attaatagaa atgtctactt aattatattt
      301 tttagtcaac attttagtta ttcttaatat taaaaatata tttaagcgtt ttacattgct
      361 atatattaat ataaatgtag ataaaactaa aaagggttct ctagaaatca atgtctatct
      421 aattatactt acttaccaat accatcactt ggtgcaaaca agactctact acactttctc
      481 cgtactctaa tataaaatca tctaattgtg tatctaaaaa attacaataa tcatacgaac
      541 cgctaatata agcagtcaat acatgtgtac atttatccat tttatttcca aacatataaa
      601 ttattatccc cctaaaaaac tttcataaaa acaaaaagaa cactatctct ttggcaactg
      661 gctgacataa ctcataagat atttatataa gtcttagtcc ctatagcttt gcgtcactaa
      721 atttctctag ttttgccgat ttagttttat tctacaacta aaataataca atagaattag
      781 ttattattca acaggattgt ttgaaagtta aataaattta tagatatgaa cattatttta
      841 cacttaaaaa acagactaaa cacgtagagt acctggtaaa aattaactat agtttttgtt
      901 ctaaaaaact cctattataa tttaagctag ataaatttga agaagtgaca taaattgaaa
      961 gaaggaatat attatgctaa ataaaaaatt accagataca gaattaaaaa ttatgaaatg
     1021 catatggaat actggttata aaactgtaat atcaaaagat gttgcagatg aaatagaaaa
     1081 aacatatggt tggaagcata caactacaat tatactttta gctagattaa ccaaaaaagg
     1141 ttttttaata tcatagagaa ttgggaggca tgtacactat acagtattaa taaaagagag
     1201 agaatatctc aaaatagagg gaaaaagagt ttttagaggt ttacataaca atcctttatc
     1261 agaattaatt tcaaaattac atgatggaga agaaataact gaagaaaaaa taatagaaat
     1321 aggaagttgg ataaaaagct ggaaatatga agattaataa ataaaaggta gctaataata
     1381 taagagctac cttttttatg tggataatac tggggataag ttgtggaaaa cttatataaa
     1441 aattgtggat aaagtgtctt gctgacattt tactgacaaa agttaaaaaa ataaatgtta
     1501 tatatattgt aaaatgttga aaaatagcca tatatataaa atataagtaa cactatagat
     1561 aaattactgt cttttatatt gactataaag aacagtaatt gaataaatat aaagatattt
     1621 tttgattatt attaattaaa aatttaattt atatttaaat aatataaaat ttgaggtaat
     1681 attatttttt aattgttcaa attatgttat tattcagata taggcaatat tagataaatc
     1741 taatgacgca aaactatagg gactaaaatc tttatgtaat aaaggtcatg tcagccagtt
     1801 gccaaaaaga taataattct ttttgttaaa agtattttta gagttatata ttttaataac
     1861 ttaatatttg gcaagaggag ggcttaagat gatatctgaa ttaatgttaa aaaattttgg
     1921 caaatctagc atcaatttta aaactaaaat taaattgaat ttaagagaaa aaccagacat
     1981 aaatagtttg aaattaaaaa gtataccaga aggtaaaatt gttaagttaa aatgtgtaga
     2041 tggaatatgg gcagaagttg agagtaatta tgacaaaggc tggttacttt ataaatattt
     2101 agaaagatta agtaatgcta aaaacaatag taatgataca ataaaaaata gaaaaaagaa
     2161 ttacatagga aatataagaa caaatggatt aagtttagaa cttagaaatg atagaacatt
     2221 agaatctaaa gttataacta caatccctga tggttttaaa gttgaagtat gttatagcgt
     2281 tggaaaatgg gcaagagtta atatcaataa aaatgaaaaa cgatattctg gatatgtata
     2341 taatcaatac atagaagagc atttaagttt ataatacgat actagtgagc tttaaatatg
     2401 ttaataaatc acaaaaaag
//
LOCUS       DACTGW010000340         2405 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.347, whole genome shotgun sequence.
ACCESSION   DACTGW010000340 DACTGW010000000
VERSION     DACTGW010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.347"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(68..631)
                     /locus_tag="I9573_003556"
     CDS             complement(68..631)
                     /locus_tag="I9573_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAT4798139.1"
                     /translation="MNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSPN
                     KRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRIL
                     HGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIALL
                     SLTSMADQLKNISEKLVDRYFPDITKK"
     gene            795..1448
                     /locus_tag="I9573_003557"
     CDS             795..1448
                     /locus_tag="I9573_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAT4798140.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            2310..>2405
                     /locus_tag="I9573_003558"
     CDS             2310..>2405
                     /locus_tag="I9573_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HAT4798141.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEY"
BASE COUNT          864 a          261 c          301 g          979 t
ORIGIN      
        1 atacaataaa agtttattta aataacattg tattttaata tttattttca gtcttacttt
       61 attaaaatta ttttttggtt atgtctggaa aatatctgtc tactaatttt tctgaaatat
      121 tttttaattg gtcagccatt gaagtcagac ttaataaggc tatcaataaa tcatctttat
      181 tttctggatt tatactaata ttttcaaaat tagactctat aaagtccggt atattctttc
      241 tttgattttc ttttattaac atgaattttt cgtaacatga aactaaatct ttttcactga
      301 aattatcctt ttcatacaca ccatgtaaga ttcttttaat ttcttgtatt gtaagtgtat
      361 ttttcataga ataaataatt atcatctgta aaatttgttc tttagtatac ttttttccct
      421 taactggttt gattaatccc tctttgctgt aattatttat catggtttta gtaagaattt
      481 tatcactttc aaatctttta ttaggagaaa gtttattgtc aattagagaa ataatttgat
      541 ccatatataa gtcaatagag ggaatatcgt ttgaagttat atctgcatta tttagtgttt
      601 ctagtattat agtattaagt tcattattca tcatatgtta cctccataat atatataata
      661 cactaaatta gaaagtaaaa caatattaag acttgtaaaa aaaacataaa gtggtataat
      721 taaatatata atatagtaat gaaaactata tttatgaatc tgatttttaa aatacataat
      781 tgtaggagga cgaaatgtat caattttttt taaagggtag agacccaata agcagtttaa
      841 ctcattttat aggtgcttgt ttatctcttt tagcaactat aattttagtt tttcaatctg
      901 ttacattaca ggaaacttct ctcttgatga tagtatctgt atcggtattt ggactttcac
      961 taattgcttt atatagtgca agttcgtatt atcatttttt aaaggggaca ccagaacaag
     1021 aattgttttt tagaaaagta gaccatgcaa tgatttatgt attgattgct ggctcttata
     1081 caccaatatg tctgaatttt atggaaaaaa aagaaggcat aatatttgtt acagcaatat
     1141 ggatagttgc atttattggc ataataataa aaatattttg gatggatgca cctagatggt
     1201 tatctaccag tatatattta cttatggggt gggcaattgt ttttgatata aatgcattca
     1261 attcaattcc taaggattgc cttagactat taattatgga aggtgtatct tactctatag
     1321 gtgcaattat ttatataatt aaaaagccta atataagtcc tgagtttgga tttcatgaaa
     1381 tatttcatat ttttattatg ataggttctt tatttcactt tttagcagtt ttattgtatg
     1441 ttttatagtt ttaaaagatt tgattaattg atactaaaat tctacaatga ataaaatgga
     1501 actgccttat atagattata tcttcgacaa taagacagtt ccttattata aatattaaat
     1561 tctacttata ataaaatttt gataaaaata gtccattcaa aatgcctatt ccataattcc
     1621 tttaatgtac ttttcattta cgacatatag tttgaattta tgtactataa ttatatcctc
     1681 taaattctct gaaacactaa caattctgac acatttgttt ctaatttcta caggtaaatc
     1741 taaatcattt gttaaattca catagtcatc atatttatac ataaaatcat ccctttacat
     1801 taagatatac ttattatact atattttaat aagttttttt aaattattac aatttttccc
     1861 gaaatttcat tttttataca taaaatttcc aaattaggta aagattaaat tttttattgt
     1921 ataaacaaaa tttacatata aaatatttta ttcaaatttt taaataacta ttgccaattt
     1981 ttgaaaaata tattattatt aatagtaata aaaaactgta aatttagaat attaataaaa
     2041 attatttggg aggaaattat catggtgaga ttaaatatag taaaattaaa tacaaaatta
     2101 aatcatcacc atcatcatag attaaaggga ttgtaatatg aaattaattc atagacacaa
     2161 gccctgtttt tagtgtaatt ggtcttgtat ctatgatggg aagtaatttt aggagaaaat
     2221 tatagccata taggtataaa gcctatatgg cttttttatt gttttaaatt tgcaaatata
     2281 agagtaaatt taatgaagag ggtgagaatg tgaaattttt gaggattgaa gatgaagtta
     2341 tttattctaa aaaaagatat aaattaaaaa gggaaataga taaacttttt ttggatgaag
     2401 aatat
//
LOCUS       DACTGW010000341         2369 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.348, whole genome shotgun sequence.
ACCESSION   DACTGW010000341 DACTGW010000000
VERSION     DACTGW010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.348"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(84..995)
                     /locus_tag="I9573_003559"
     CDS             complement(84..995)
                     /locus_tag="I9573_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HAT4798142.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SVGFPLYSKVGAAGGYHVLKERILPPITFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENMRRKLVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSIRTIVPIGLYAMNGLWYCPAYCIKSESVKEFRADRIVKILSIENLSNKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKK"
     gene            1106..1573
                     /locus_tag="I9573_003560"
     CDS             1106..1573
                     /locus_tag="I9573_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798143.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIEESYNFAKQKFKKRKNK"
     gene            complement(1741..2340)
                     /locus_tag="I9573_003561"
     CDS             complement(1741..2340)
                     /locus_tag="I9573_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798144.1"
                     /translation="MKLKRTLFVGLALVLAIGITACARDNEKPNENAGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKRMNDDLIAEGERLVKDIDERIEKLDKVSDEDMKKGQN
                     DFMKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT          794 a          397 c          281 g          897 t
ORIGIN      
        1 ttatcctatc tctacatata agtttcccta tatgctgact aatttagcta ctaactcatg
       61 actaaagtca caagtgtgcg tagctatttt ttaatcaaat gtttaagttc tataggttct
      121 attattgtag cattttttcc aaaagttaaa aaatattcag ccacccaatt aagtgtagat
      181 tttggtatat acatatctat gtatccaccc ttagacaaaa ttttcaatcc tcttgccaac
      241 aaaaattctg tttcacaacg ttttactccc tcatctgtca gattaatttt aatatgataa
      301 tctgtaccta cttccatatt cttaagataa tcatgtatag aagataaaac cttatatttc
      361 ttatttgata agttttctat actcaaaatc ttcacaattc tgtctgcacg aaattcctta
      421 acagattctg attttataca gtaagctgga caataccaaa gtccattcat agcatacaaa
      481 ccaataggaa caatggtacg tatactattt ttttgctttg atgaatattc aattttaatt
      541 gtaaattcat tcattactat attaaacaat tccttcaata atggtgttga gcaatgccta
      601 tctggtgtcc aaaaaacaag tttacgtctc atattctcaa tattatattg aatatcattt
      661 ggtatacaat ttaaaaattt ctttaaaaca gaaatagttt cttgctcaaa tggtaaatca
      721 ttataatact ctagtgactg atatgcaaag aatatagatt ttacctcact ctctgaaaat
      781 gtaattggtg gaagtatacg ttctttaagt acatgatatc ctccagccgc tccaactttt
      841 gaatatagtg gaaatccaac actttccaat acctgtaaat ctcttagtat agtacgtttt
      901 gatacagaaa attcttctgc tagctctccc gctgtaaaac ttctttttgc atttattgtt
      961 atcatcagtt caattaaacg ctcagctctt gacatattca tactcctaat ttaaatatag
     1021 tgacaataat tgtcaccatt atatgataag atattcttgc atctaaagca ataataatat
     1081 taaagaaaga agggattgat attttatgga aaatgatact attaaggctg atgatattct
     1141 caattattgt ctatcaaact tagatgatgt tgtactaatg gatagttggg gggaacgagc
     1201 aatttactac aatcctaatg gtgttttaaa gcgaggggta tatgttctta ccattaaaga
     1261 aaaagacagt aatcatgata aaggttcgtt ggttagtcgt ccaaatgtat accgtgtgaa
     1321 tataggatta aaaaaagaaa cttttattga aatgtttgga tatattccaa agcgtccagg
     1381 tgtagggcaa atagttgata tggattttga ttttacaaaa ttggacacaa tcatgccaca
     1441 tcctatctac tcatggatgg gatggatatg cgtcctaagc cctactgaaa agacatttga
     1501 gaactttaaa actttaatag aagaatctta caattttgca aaacaaaaat ttaaaaagag
     1561 aaaaaacaag taaaaacata aaaaaataac ctctaaataa actataatga aaatatgagg
     1621 ttacaagata aaaattagta tcttgataac atacaaaaaa gcgaagccaa aaaatatatt
     1681 aattacaagg cttcgctata tttcaaatat ttatttctaa ctttaaaact atttactcta
     1741 ttatttttta ttatttcctg ttattcccaa tctatcattc atatctttta tcatttttct
     1801 aaaaccacta ccaatatcgt ttccaacatc ttcagttcca tgaactagtt tcataaaatc
     1861 attttgacct tttttcatat cttcatcaga taccttatct aatttttcta ttctctcatc
     1921 tatatctttt actaatcttt ccccttcagc tattaggtca tcattcattc tctttaactc
     1981 tgtatcttct gttttaccgt cttttttaag agatgttaca aatgattgta ttttatctct
     2041 actatcttta tatgcagcct ttacatcact tagatatttt tcatttcctg gatattcttt
     2101 atctttaaac tgattgttta tactttctgg agtatcataa atagaatatc tatttagacc
     2161 tgctatataa tcattataac ttgttgaata ataattcata tatccttcat tattatttac
     2221 agattcatta ggattttttc ctgcattttc atttggtttt tcattatccc tagcacatgc
     2281 agttattcct atagctaata ctaatgctaa tcctacaaat aaagttcttt ttaatttcat
     2341 ttttattacc ctcctagatg ttttgtact
//
LOCUS       DACTGW010000342         2362 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.349, whole genome shotgun sequence.
ACCESSION   DACTGW010000342 DACTGW010000000
VERSION     DACTGW010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.349"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            30..1034
                     /locus_tag="I9573_003562"
     CDS             30..1034
                     /locus_tag="I9573_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT4798145.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNAALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGIKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            1180..2271
                     /locus_tag="I9573_003563"
     CDS             1180..2271
                     /locus_tag="I9573_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAT4798146.1"
                     /translation="MACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKSKQSESKS
                     VPTSAEKDKLNSKSKNEEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLSALKRADE
                     DFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYPKQQFAIV
                     DYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDKYESGFRK
                     GLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRAIQVAREN
                     GGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEFKIKDGEL
                     GLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
BASE COUNT          955 a          251 c          453 g          703 t
ORIGIN      
        1 tatcgtaaat gtatgaggag tgaaatttaa tgcgtttcaa aagaatattg atattgatgt
       61 taactgtagc aatgatagct ggaatgttgg ttgggtgtgc taataaccca agcaataatc
      121 ctagctctag caaagataat aaaagtgata taagcgataa aagtaattca aaaaaatctg
      181 tatcaatgat actagatatt gaaggaacaa ataatgaagc tatgaataat gcagctttat
      241 tagcattaaa caatgctcaa aagaaactta atatagatac aaacaaagtg gaatcagatg
      301 attcctcaac tttttctaat agtatagata ttttatgtaa tgacaattac gatttaatta
      361 tagctgtagg agctagattt gcaaaaccat tggagatggt agccaagaaa tatccaaagc
      421 aacaatttgc aattatagac tatgagtatg acaagcaacc ttctaatata acttcaattt
      481 cttatgaaga taataaatct ggatacctag ctggattaat tgctggaaaa atgactgaaa
      541 gcgataaggt tggttttata ggaggaataa agagttctag tagggataaa tttgaatcag
      601 gttttagaga gggtgttaaa ttttctaata gtagtataaa ggatatttca gttgagtatg
      661 cagatgtttt taaagatagc aaatcagtag aatctatagc taaaaaaatg atggataatg
      721 gagtggatat tattttttct actactgaag atgattcaaa agctgtaata gatgcagtaa
      781 gggcaaaaaa taaaaaagtt atagcaacga ataaagacca acatgagtta gctccagaaa
      841 atgttataag ttctattgta aaagattttg agaatcctac ttataatctg atacaaagtt
      901 ttgtaaaagg aaattataaa ggcggaaaag ttatagaaaa cacagttaag tctggaagta
      961 ctgggcttgc ccaaaatagt tctcaaaata ttcctccaga tgtgttagaa tatgttaata
     1021 agaacaataa ataatctact ataatatcca agaatactta tgactttagt tatgaggtga
     1081 attggatgtt ataatagaca tatatagaaa gacgtatgca gaggtaggca gacctatcta
     1141 aatcagggag tgaattttgt gaaaataaaa aggatattga tggcttgctt tgtagtagtt
     1201 tttacaattt ttatattagc tatatgtaac tttgattttg gatttgataa agtaaataag
     1261 aataattcta atatagatag tgctaaatcg aaacagagtg agtctaaaag tgtaccaact
     1321 tcagcagaaa aggataaatt aaatagtaaa agtaagaatg aagaagatga cacagaagat
     1381 gattcatatg taagaaaaac tattggagta ttgttagata ccgaaaactt aaataacgaa
     1441 tcatataata ataatgtact atcagcctta aaaagggctg atgaagattt tgatgttagg
     1501 ataaaacttg aaaaaccaaa gacttctaaa agttttaaag agagtataga aaaactacat
     1561 aagtatgaac cagatttgat aatagcagtt ggagctagat ttgcagagcc aattaaagaa
     1621 gctgctacta aatatcccaa acagcaattt gcaatagttg actatactta taaagttcag
     1681 ccagctaatg taatttctgt atcatttcaa gataatgtta ctggatattt agcagggctt
     1741 attgcaggta aaatgactga tactgggaaa gtcggtttta taggcggagt tgagggttct
     1801 agtagagata agtatgaatc tggatttaga aaggggctta aaacttcaaa taaagatgct
     1861 gagttactag ttaagtattc aaatatattt gaaaaccctg aatcagctag aactattgca
     1921 aaggaaatga gagattctga tagagatgtt gtattttcag ctacagaaga tgatagtaaa
     1981 agagctattc aggtagcaag agaaaatggt ggtaaagtta tagctattaa taaagaccaa
     2041 tacgatatgg ctccagataa tgtaataagt tcaattataa aagatgtaga acaacctgtc
     2101 tatgaactga taaagaacgt tgcaaaagat ggttttaagg gaagtaaagt aatggaattt
     2161 aaaataaaag atggagaact tggcttgact tcaaagagca gtagaaatat accaccagat
     2221 gttttggaat acataaaaaa agagtataac aaagtgaaag atacatatta attattgatg
     2281 tagaagatta taaaaatata ctgtttaagt ttagattagc aataaaaaga tatatttatt
     2341 aaatttgtgc taactgtaca aa
//
LOCUS       DACTGW010000343         2351 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.350, whole genome shotgun sequence.
ACCESSION   DACTGW010000343 DACTGW010000000
VERSION     DACTGW010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.350"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            40..282
                     /locus_tag="I9573_003564"
     CDS             40..282
                     /locus_tag="I9573_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAT4798147.1"
                     /translation="MQNSMKQEPCDFSHGRFRFKTSDNRVLIPVVIFLGSIVTALCDL
                     ISRVILSPVELPISTITSLLGAPIVVMLLVKRKDRA"
     gene            285..1481
                     /locus_tag="I9573_003565"
     CDS             285..1481
                     /locus_tag="I9573_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4798148.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(1601..2326)
                     /locus_tag="I9573_003566"
     CDS             complement(1601..2326)
                     /locus_tag="I9573_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAT4798149.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTPFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT          883 a          278 c          384 g          806 t
ORIGIN      
        1 ggggatggcc tgtggagaat tagtaagaca tattttagta tgcaaaattc tatgaagcag
       61 gaaccctgtg actttagtca tgggaggttc agatttaaaa catcagataa tagagttttg
      121 ataccagtag taatattttt aggcagtata gttacagctc tttgtgattt aatatcaaga
      181 gtaattttat caccagtaga attaccaata agcacgataa catctttatt aggagctcca
      241 attgtagtaa tgttattggt gaaaaggaag gatagggctt agacatgttg aaaactaata
      301 atttaagtgt aggatacaat aacaaggtag ttataagtaa tataaatgta gaagtaaaaa
      361 atggagaaat actatgtctt ttgggaagta atggagctgg aaaaactacc ctattaaggt
      421 ccttatctaa acttatatca ccaataaaag gtgaaatata tttgaatggt gtaaacataa
      481 attgtatatc aagaaaagca ttatcaaaaa aaatggcttt agtacttaca aatagattat
      541 taggtgattt aatgactgta caagatatag tcaatatagg aaggtatcca tatacaggat
      601 tttttgggag tctatctaag aaagatttaa taatggtaga tgaagcattg gagtctgtag
      661 atgccttaca tttaaagaaa agatattttg atgaactaag tgatggagaa aaacaaaagg
      721 tgttggttgc aagagctctt gttcaagaac cagaaataat tatattagat gagcctacaa
      781 ctcacttgga tataaaacat agattggagc tcataaacat actgaaaaaa ttaagtaaag
      841 aaaagtcaat atcagtaata ctttcattgc atgaaataga catagcatta aaaagttgtg
      901 ataaagttgc tcttataaaa aataataaag tcatagctta tggtcagcct gaagatgtag
      961 tagatgagga tattataaat tcgctttatg aattggatga taaaaacttt aatagtcttt
     1021 taggttcagt agaaattagt aataaatcta aaaatgaagt ttttataatt ggtggtgggg
     1081 gtaaagcaac accaatttat agagctttta ctaaaaaagg tataggtctg tattctggaa
     1141 taatacatga aaatgatatt gattatgaaa ttggtagaac tatggggata aagatgttta
     1201 cagaaaatcc atttgagccg ataagtgatg agagtttcga tttggctatt agaaatttaa
     1261 atgattccaa gattataata gatacaggat ttagtgtagg agagacaaat aagaggaata
     1321 tagatattat aaaagaggct ttaaaactgg ataaaaaagt ttattctttt agaaatagag
     1381 atgagagtaa gaagtattat gacagtttgg atagtaagat agaacatata gataaagttt
     1441 ctcaaataat caatagtgca gatataaata atttattata atttatgaaa taaaatgtac
     1501 ttatgtgcta tagaaaaaag agtatagatt ataatttttt tgaaattaca atctatactc
     1561 ttttgttatt ttgtttatat atttataaaa ctttagcatt ttatagataa ccaatagctt
     1621 cctctctagt taaaaaaaag tgaatccctg tagtcgattc tttccatcta ttttcattaa
     1681 aatcttcagc tacaatcgtt tctcctactc tatatataaa attttcatca gcatatgcac
     1741 gagcactttt aaaagattct ttaccatcta tactagtaat tgacagtact tttgccttat
     1801 cacatctaca agcatttgat gtagcagaca ctctttttgc gtcgctgggt attaataatt
     1861 gtaccattct gaaattaaaa cattttttat agcctataaa tgctcctgtt tcaggacaat
     1921 acaatttaaa aaaaggggta ctttcatctg atattactcc atttaaagta gcatgttcta
     1981 aattggaatt ttctaaatta gctcctctaa tatctgagtt agataagtca caaaatctaa
     2041 aatttgcacc acttaagtct gcatttttta atatagaatt ttttaatgaa caatccgaaa
     2101 gataagaatt attaaaaata gagttttcta aatctgcttt ttctaaattt aaattgataa
     2161 ggtcagacca cgaaaagtca atattactta agcagtatcc tcttaagtcc atattttcta
     2221 atcttaattc ttttaaatct gccttttctc cagcattatt gtttttaagc cataaataat
     2281 gtttctcaag tgttttttct aattcttgtt tagaaatagt tttcattaaa atcaccttca
     2341 taaatttatt t
//
LOCUS       DACTGW010000344         2310 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.351, whole genome shotgun sequence.
ACCESSION   DACTGW010000344 DACTGW010000000
VERSION     DACTGW010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.351"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(87..2276)
                     /locus_tag="I9573_003567"
     CDS             complement(87..2276)
                     /locus_tag="I9573_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HAT4798150.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEYNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
BASE COUNT          708 a          429 c          225 g          948 t
ORIGIN      
        1 tttttacatc aattgatttc ctcagaaaaa taggtgtcat aaagaaatgt atggaatcac
       61 tttacaacac ctgtcataat aaaaaatcac tctggtagtg cttttttatt tacttttcct
      121 attggtgtaa ctggtatttt atctatcctc attattattg ttggaatcat atatggaggt
      181 agatgctcat ttaaatattc tctaatggtt ctttcattta tcttaccact atcttcctca
      241 tcaattacat aatatgcaca tatatacata tccccgtcat catttgttct cgatatacaa
      301 acaacacttt taatttctgt aatattattt attatatttt caatctcata tagctctatc
      361 ctaaactcat caatttttac ttgattgtcc atccttccaa tataatctaa atttccatca
      421 tctaaccatc ttactaaatc accagttcta tacattaact tattatattc acaagttttg
      481 taaggattct ctacaaatac ttgctctgtt agcccttttt tatttaaata tcctcttgat
      541 atttgaatgc ctgatagaca tagctctcct ggtgctccaa ttggacatag tttgttcttt
      601 ttatctaata catatgcata agtattaaat ataggttttc ctattggtat ttttccatat
      661 tctctgtcca catgaaaaga agtcgataca acagtaaatt cggttggtcc atactcatta
      721 tatagttcat aattacgctt tctatatcct tttaatcttt ctcctccagt ctgtacaact
      781 ctaagtgaat tattattgca attttgtata aataaccttg caaatgctgt tggcaatgct
      841 agtatagtta tgtcattttc ctcacagtat tcatttactg tatattcatc aaatttagct
      901 tcttcaggca taatatctat tgatgcacct acaagcaaat aagggaatag ttgtttcatt
      961 acagcatcaa aactgaaact taagtatata ccacatatat cattttctgt agcgcttata
     1021 gattttacgc tataataaca catatttact aaactcttat gttctatagc tatggtcttt
     1081 ggcctaccag ttgaccctga agtagatatc gtatatgcac aatttgatga tgttattttt
     1141 tcagttaatt catatccaag cttaaacatt tcttcatcat ttatatcgag tactatggta
     1201 ttctcattta caaccttact tttgagttca tctgtagtta tcactacttt tgcattacta
     1261 tcttcaagaa ggtaattcaa tctttcttct ggattactcg tgtctattgg aaaaaatgct
     1321 ccccctgatt tcataatccc tattgctgta ataattatgt taatatctct tggtaacatt
     1381 acagctacta tgtcttcact ttccactcct atatgattta aataacctgc aagtttatca
     1441 gttacctcat ccaattcttt atatgttaac tttctatttt catccctagc agctatatta
     1501 ctaggtgtta ccttaacttg ctccctaaat aaatcaacat aacttttatc atagggtact
     1561 tcatattgat tattattaaa gtcttccaat aatacttttt cttcttcctt acatacaaat
     1621 tctaaatctt ctattaaaat atcattatct tctattattt gctctattat ataatcaaat
     1681 tgatttaaaa ttcttctaat agtttctctt ttatatatag atggtttata ctttattttt
     1741 attttaagtg ccctcttagt atatactatt acagataaat catatccttt tttttctatg
     1801 tcataatcat atgataaatc aaaactttta gctataaata ttgtgtttat aagcttagta
     1861 tttagatgat ttttattctc tatatctgat aacaaatagt atccatactc taaagacctg
     1921 ctccactgcc tttttagctt ccttacaaca tctataaatt tttcactttg tctaactctt
     1981 actcttattg gaattatatt cattgtttta ccaaaaacta catcatcttc atatatatac
     2041 ttttgaagta taaggccata tatagtttct atgatagagt ctatgctaat attgttcact
     2101 ttagaatatt gaattaacct ttcatgtttc tcaatattca tcttaaaaac ctcatctact
     2161 acttcatcag aacaatcttt ataaaaaaaa tcatttggaa tttttgctat attcgtataa
     2221 tcatctaaca aatccatcca ataattcata gctcgttcta aattaacttc atacatatca
     2281 aaattccccc ttactactaa gtctatagaa
//
LOCUS       DACTGW010000345         2301 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.352, whole genome shotgun sequence.
ACCESSION   DACTGW010000345 DACTGW010000000
VERSION     DACTGW010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.352"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            485..2296
                     /locus_tag="I9573_003568"
     CDS             485..2296
                     /locus_tag="I9573_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HAT4798151.1"
                     /translation="MKKKYDFLIYSAIWLVIIIFFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILYNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
BASE COUNT          918 a          210 c          359 g          814 t
ORIGIN      
        1 taagaaataa gtaaattatt ggtcttattg cacaaaaata gatataattt aagtaatata
       61 ccaaaaaaaa ggaatatatt tacataatat gataaaataa attcaaagag ttaaattgta
      121 ggtaaatagt attttaatta tttattaaaa tatagattat gtatatgcca aatttaaaaa
      181 gagacataaa gacattatac aaatatatta ttggggtgat attttataag aaaagaagtg
      241 atgcagtaaa ttaattgagc aatttaaaaa tgatttaatg atagtgttga tgaatgttta
      301 aaaagaaatt ttagaaaaac gtatattgct gtttgtgttg gaggtgatga atttgtataa
      361 tttatagggt ataaatgatt aaactactta agataaatac atatcactgt aaaatattga
      421 taatcatttt ttataagaga ttgattattt gcaaattata tatattacaa atgaggtaac
      481 aataatgaag aaaaaatatg attttttaat ttattctgct atttggctag ttattattat
      541 tttttttata ttttctctat atcatagtat agagcatatt aaaattataa gtcacactgg
      601 tactattcgc agtgaaactc aaaaggttgt taagcaagaa ttgaataatg aaagaaatga
      661 tgacttgata aaacgactgg ataatattct gataaagcta agaacaggta atggagaaaa
      721 tggatttcaa agatgtgata ataaagaatt tcaacaaaaa ctaaatcaaa tggattctat
      781 gtgggaatct atgaagaaag aaattataaa agttagaaat ggtgcttcag gtgataaatt
      841 atataaattg agtgaagagt attctgtgtt gtctaatcaa attgttttta tatctgaaaa
      901 acactctaat gcaaaattgt attcttttgc tactgcacta tttatttatc ttatattctc
      961 aacaataagt cttttaattt gggaatatta taataaaaaa aggtttaaaa gaatatttta
     1021 tactgataat ttaacaaaaa taaaaaatca ggttgcattt gaaaacagag cgattgaaat
     1081 tttgtacaat gcatctaaca aagagtatgt actgctaaat attgatattg acaattttaa
     1141 atatattaat gatacacatg gatatgaata tggagataaa gttctaataa tagttgcagc
     1201 tgcactttct aaaactttta atatcaaaga aacttgtgca agaattggtt cagataattt
     1261 tgtaatactt gcaaaatata gagattctct tttagaggat attcgggaaa tgctaactaa
     1321 tgctataatc tctgaattag atatgaatgt aactcaaact atatcttatt gtataggagc
     1381 gtacttagtt gaaattgata atttaggtta taaatccatc aattctataa tggataaggc
     1441 aaatattgca cacaaggtaa gtaaaacaag aggaatttcc tccacagtat ggtataatga
     1501 aaatctgtta aaacagttac aaatggaaaa tagtatttat aattatatgt ataaagcgat
     1561 agaaaatgaa gaatttcata tgtatttgca acctaaattt caaatatctt ctttaaatgt
     1621 tgtaagcgca gaagctcttg taagatggtt ttctccagaa cttggttttt tatcaccaga
     1681 tgaatttatt ccattgtttg aaaaaagtgg atttattatt gaattggact tttatatgtt
     1741 aaaaaaagcg tgttcatttg taaaaaaaac gtttatgaaa aaaaatcaat atacatatcc
     1801 gattgcagtt aatttttcaa gggttactat ttaccaaaat agcttttatc aaaggttttt
     1861 agatactgtt aaagaatatg aaattccttt taagtatata gagatagagg taactgaaag
     1921 tgcttttaat gaaatatctc aaccagttat ttcgatttta gaggagctta aaaagttagg
     1981 atttttaatt tcgatggatg attttggatc aggatactct tcattaagtc ttttatgttc
     2041 tctatctatt aatggattga agttagataa aagcttactt aaagaaacat ttaaccggga
     2101 gaaagtatat agtattattc agtgtattat tgaaatgtca catcgtatag gcatgtctgt
     2161 tgtatgtgaa ggtattgaaa ctaaaaaaga cttggaattt ttaaatacag ttaaatgtga
     2221 tgtaggacaa gggttttatt tctctaaacc gatagaagaa aaagaatttt ttaacaaata
     2281 tgtaactaaa aaataatata a
//
LOCUS       DACTGW010000346         2268 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.353, whole genome shotgun sequence.
ACCESSION   DACTGW010000346 DACTGW010000000
VERSION     DACTGW010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.353"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..185
                     /locus_tag="I9573_003569"
     CDS             <1..185
                     /locus_tag="I9573_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798152.1"
                     /translation="IFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCN
                     EPLMFRVIISIDSYDA"
     gene            225..1199
                     /locus_tag="I9573_003570"
     CDS             225..1199
                     /locus_tag="I9573_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HAT4798153.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            1305..1964
                     /locus_tag="I9573_003571"
     CDS             1305..1964
                     /locus_tag="I9573_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAT4798154.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            1967..>2268
                     /locus_tag="I9573_003572"
     CDS             1967..>2268
                     /locus_tag="I9573_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HAT4798155.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKS"
BASE COUNT          930 a          239 c          300 g          799 t
ORIGIN      
        1 aaatatttaa aaagatagaa aatatattaa tagactctaa agttaattta aaaatgggtt
       61 gggaaagaat acttataaat tatatcttaa aagatttatt tgaattaaat tatgaaataa
      121 ataaaagttg taatgaacct ttgatgttta gagtcattat atctattgat tcttatgatg
      181 cttaggctca ataaataatt tacctgagga tgtgctttag atttatgaaa tacaagttat
      241 ttatagactt tgaatttaat atactagatg ataataaaaa taaaccaaaa gaatataatg
      301 gtgcggaatt aatttctatt ggtggagttt tagttgataa cgaatttaat acaatagata
      361 attattactc tttagttaag ccaaaataca ataaaatact ttcaaataaa tgtaaaaatt
      421 taaccaaatt gaaccaacta gatatcaata atgctcctaa tcttttatat gttatggatg
      481 atttttataa atggttttgt aaatttgatg atgttacaat atataattgg ggtgattttg
      541 atataactgg ccttctaact tcatttagag tatataaata tacaggtaaa tgcttagagt
      601 tatttaatat gatgattgac attcaacctt ttatatctca acatattaca tacaataata
      661 gaatcttatc taagcaaata tctttactga atatgaaaaa aattttttct gtagaaggag
      721 atattaaaca taactcactt tcagatgctg tggatttaat gaacgtatgt aaatgctttt
      781 tctttaactc ccctaaaaat atcgatattc ttgaagaatt atataataaa ttacctgctc
      841 tcaaaacacc tttatattat attccctact ttgaagatga aaattttgaa ctcaaatttg
      901 atagaactcc agaagatata ataatatatc ttaaacaaat attaactata ctaaatataa
      961 gtaaaaaaga aatttatttt aaaaaaagaa gtgtattagt gaataactct aaaatcatta
     1021 actttaaaaa tctatcttct tctttcaaat taataaaact caatgaattt gaagaatatc
     1081 ctgattttat cttaaaatta ggagataaaa agagttttgt tgaatctgtt attaaaataa
     1141 ataaaagtaa cagaaaatcc ataaaaaatt taatattaaa attatcaaaa aaaccttaaa
     1201 tagttgaatc caattattta aggtttttta tactttgaat taaatttttt atataggata
     1261 tactaactaa gtgtaaatat tttttaaagg agtaattgat agatatgaca gtagtattaa
     1321 taagaagtat tatattatac ataacagtgc taattgcact tagagtcatg ggaaaaggtg
     1381 aaatagctga aatgaactgt tttgatttag ttataacact attaattgca gaagttgcat
     1441 ctgtcccaat ggaaaataat aatataccaa tcataaatgg tgttgcagca atatctggtc
     1501 ttgtaatcat gcagactctt atttcattct tatcattaaa aagcagaaaa ttaagttcat
     1561 ttttatcagg taaaccttct gtattaatag ataaaggaaa aattgtatat aaagagctta
     1621 aaaaagaaag aattagtata gatgaattat tagaacaact taggattcaa ggatacttca
     1681 acctaaaaga tgtacaatat gctattttgg agactgatgg taatctaagt gttgtaccag
     1741 catcaagcta caatagtact cctccaagag cttttaatca tttacctatc ccacttatac
     1801 tggatggtag aataataaat aaaaatctag acatagctca aaaagatact aactggttaa
     1861 tgggtatttt aaaatctaat cacatagaaa cttttaaaga tgttcttatt tgtgttttag
     1921 atgaaaatga caaaatattt attcaaaata aaaagggtga ttaatattga aatctacaac
     1981 atttgttata ctatggacta tcttatttgt attatttgga ttttacgtta acaataaact
     2041 atatgatttt acagaaggat ataaagacaa tatatctgtt ttagaaaaat ctatagaaaa
     2101 tgaagattgg gaaaaagctc aaaaagaagc tgattcaata tctacgagtt ggaataaaga
     2161 aaaaaaccat tggtacaaag ttttaaatca tgagtacttt gatgaaatag gattaaggtt
     2221 taatattctg gacaaagcaa tttatactga aaataaatta aaatcttt
//
LOCUS       DACTGW010000347         2212 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.354, whole genome shotgun sequence.
ACCESSION   DACTGW010000347 DACTGW010000000
VERSION     DACTGW010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.354"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(38..1330)
                     /gene="brnQ"
                     /locus_tag="I9573_003573"
     CDS             complement(38..1330)
                     /gene="brnQ"
                     /locus_tag="I9573_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAT4798156.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIKFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          733 a          371 c          242 g          866 t
ORIGIN      
        1 ttaaaggcaa ttccacaatt atcaattagt tttattatta tgcagtttta ctgcttttag
       61 atggaataaa gtttcctatt attcctgcta ttacaactgg aactatccag ttaaatccta
      121 atgaagcaaa tggtaagcta ttaactagag gaatttttac accaaaactt gttgcaactg
      181 ttaatacact tactaataat gccatgtatg cagcacattt gaacacgttg tcatttttaa
      241 ttttattact aaataatgct agtataacta gagttatagt tgctggatat accatactta
      301 gtataggaga agagaatttt attattgtac ttactccaaa gtttgatatg attgcactaa
      361 atacacaaac tactataact atatgctcat actttaattt tccatctgta agtgttgaga
      421 aatattgacc agctgctgaa gaaagaccta tagctgttgt taaacatgct aatgcaacta
      481 taatagccaa taaaatttta cctgtagttc caagtaaagc tgctgttatt gcaactatta
      541 aagctgtttg ttgaacatct tgtccatatt gttgagacac agttgctcca agataacata
      601 atccaccata aactaatgcc attcctatta ctgcaattac acctgcactt attgtggctt
      661 tcatcttttc tttctcacct gtataacctt tagctattac tgacatgatt ataattgatg
      721 caaatacact agctgccaat gcatccattg tttgataacc ttgagttata ccttctgcaa
      781 atacattttg aattaaagaa ctgtctctta tttctcctaa tggagatacg atacctttaa
      841 ctattaatac tgctaaagct ataagtaaag ctggagttaa tattgaccca actatatcta
      901 caacttttga tggtcttatt gttaaaacca atactagagc aaagaatact atagagaata
      961 ttactggact tattccagaa cctattgtag gcaatatacc catttcataa gttgttgctg
     1021 ctgttctagg tattgctaaa agtggcccta tacagattac cattgcacat cctaatgcaa
     1081 tacttaatcc tttgcctgct cttgagaaca tcttcgatgt gtctccattg aacttagttg
     1141 ctgctataac tgctaataat gccaaaccac catctgcaaa aaggaatcct atgaaagcta
     1201 ttagccaact acttccagat aaaactccta gatatggagg gaagattaag ttccctgcac
     1261 caaaaaacat tgcaaatagt gcaaatccaa ttacaattac atctttaaaa ttgatttgtt
     1321 tctcacccat ttaaagcacc cccatttgaa ttttactacc ttttactaaa aaataagtcc
     1381 aatctcttca ttaaatgaaa accttttgac gagattttat ttaattatac atttttgact
     1441 taaatttgtc aattatgttt cataatttat aaaatcttca caatatttaa tacaactttc
     1501 ttaattaaac aaaatcaacc tgtatacttt aaacatagtt ggttttttgt tttttctcta
     1561 attttccttg ataaaattaa ctattcagaa caattaaaat attaagactt tttagattat
     1621 tcgttttatt attaagttgt attgctttat atactgtata tatcactctc ctttttacat
     1681 tcatataatc tttttaattc aatttaaatt ttaaatattt tgtgtttatt atataatctg
     1741 aaaaaaaagc agtcaatagc atttttaatt tttttaattt tcaaaattat gattcttaaa
     1801 aaatcgtttt ccttttatca taacacctat taacgttaat tcttgtagta ttttgtattt
     1861 attttgctaa attgacagtt aactttattc aatttttatt ctactcacgt agatttacct
     1921 cttgtctttt agtcttaaaa ccattttttc tgaaaattcc ttattatgat ttatcaactt
     1981 atttttcttc atagcaatta ctttctaact tccaacttac ttaaactttg cagttgctag
     2041 tattcacgtt tacatactta tatgtcataa ttaactaaaa attcttaata tatctctcaa
     2101 tgaaaacaat ttactcattt atttaataca tttatataat catatgtctt aaattttgtt
     2161 ttattatttt attattgtca caattttatt tatattatat ttttgtaaaa at
//
LOCUS       DACTGW010000348         2206 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.355, whole genome shotgun sequence.
ACCESSION   DACTGW010000348 DACTGW010000000
VERSION     DACTGW010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.355"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            99..1316
                     /locus_tag="I9573_003574"
     CDS             99..1316
                     /locus_tag="I9573_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAT4798157.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            1478..2176
                     /locus_tag="I9573_003575"
     CDS             1478..2176
                     /locus_tag="I9573_003575"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAT4798158.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT          848 a          255 c          340 g          763 t
ORIGIN      
        1 tattttacta tacatacaaa attatttgaa agttcaaaaa ttataagtat aaaagctaat
       61 cttagcttaa ataaaaatta tatttttagg aggtaaatat gttaggaaaa aaatgtatgg
      121 attatctaca aacattagga aaaataagtt caacaacaaa tggtcttaca agacttatct
      181 tgactcaaga acataaaaaa tctattgatt taattagttc atggatggaa ggtttaaatc
      241 ttgatattga aattgatgac attggaaacg ttataggtac atataaatca agctttccaa
      301 atgctcccac attagtagtt gcatctcatc aagatagtgt aaagtgtgga ggtatatttg
      361 atggtatgtt aggtataata gtgcctctag ttggtcttga agaagcaaaa cacaataata
      421 ggtcttatcc tttcaatatt aagctaattg ctttcgctga agaggaagga actagatttg
      481 aaacttcttt aatgggaagt aaagtttttg ctggtacttt taaagaggaa cttcttaaat
      541 ctgttgatga aaatggaatt acacttgaag aagctgtaac aaagtttgga tttaatacaa
      601 aaaacttaac aaatttgcat cctagaaaag atgtagatgc atatttagaa tttcatatcg
      661 agcagggtcc tgttttagaa aatgaatctc ttcctgctgg aattgtatca agtattacag
      721 gatttaaaag ttttaagata tcagtaaatg gtaagtctgg acatgctggt acacttccaa
      781 tgaatatgag acttgatgct ggatgttgtg cctgtgaatg tgtattagct atcgaaaaag
      841 tagctaaaac gactgctgat ttggttgcta ctgtaggaaa gatgaatttc tatccatctt
      901 catcaaatgt ggtacctgaa agagctgaat ttacactcga tgtacgctct tgttcacaag
      961 aaattcttga taattctgta gaaaagatat ttaatgaaat atcacatata tgtgaaaata
     1021 gaaaacttaa ttatacaagt gaacttgctt ttgaaaatgt tcctgtacca tgttctaata
     1081 aaataacaaa aatcatagaa aaaagtttta tagatttaaa tcttaatcct ttttatattt
     1141 atagtggtgc tggccatgat gctcaggaaa tggataatat aactgatatt ggtatggtat
     1201 ttataagatg tgctgggggc gtaagtcata atcctaatga aagtgtttct gtagatgact
     1261 tggatactgc tgtaaaaata tttttaaaaa tactagataa tcttgattta aaataatttt
     1321 tatataagaa agtatattaa aatatataat ctaaaaaatc actttatatt tatattatac
     1381 aagtgtacaa tataatctat tataaaatat aattttatgc ttttatagat ttttatttta
     1441 tacataaata tatttatata taaaggagat aatttttatg aatgaaaaat taattggtat
     1501 tttggctgga atgggtccaa ggtcaacagc cccttttgtt gatttaatta ttgatgagtg
     1561 tcaatctcaa tatggagcaa aatatgatga tgaatttcca aagatgatga tatattcatt
     1621 acctacccca ttttatattg accgtccaat aaatcatgaa cttatgaaag aaactataat
     1681 tgatggatta caaaagcttg aatcaactgg tgtaaatttt attgcaatgc catgcaattc
     1741 agcacatata tactttaaag aattaaaaga gtctattaat ataccaatat taaatattgt
     1801 agaagaaaca gttaaaaaat tacctactat ctcacaaaaa gtaactcttt tttcgactag
     1861 ttcaactttt gagtctacaa tttaccagaa tggtataata cataatggac atgaatttat
     1921 atttaaagat gaatggcaaa taaaattaaa caatcttatt caaaatataa aaatggataa
     1981 agaaaatcca cacaatattg atatttggaa taaacttatc gaagatgtta aaaatgaatc
     2041 tatagaaaat atagtaattg cttgtactga tttaaatgta gttttagaaa aatcatatcc
     2101 ttcaataaat attgttgatt catcaaaatg cttggctgaa tcagttgtca ataaatatct
     2161 aaaattagtt aaataaattt ttcatgcact atattataaa aaatat
//
LOCUS       DACTGW010000349         2204 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.356, whole genome shotgun sequence.
ACCESSION   DACTGW010000349 DACTGW010000000
VERSION     DACTGW010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.356"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(99..950)
                     /locus_tag="I9573_003576"
     CDS             complement(99..950)
                     /locus_tag="I9573_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAT4798159.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(999..2162)
                     /locus_tag="I9573_003577"
     CDS             complement(999..2162)
                     /locus_tag="I9573_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAT4798160.1"
                     /translation="MFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKLNR
                     FIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNPYD
                     YLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPQGELAKMTKNDEN
                     NYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVIDRK
                     DEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPKSF
                     IDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFKEK
                     YNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
BASE COUNT          666 a          417 c          239 g          882 t
ORIGIN      
        1 taaatgaaaa aaatacacca tttacatata attaatataa atttttcata taacaaatat
       61 cctaaactta tctaaaataa atttaggata ttttattttt atactctatt tacagaccca
      121 cacactttaa tcttttcttt tacaaaatcc ttcactgatt gaagccctgc taatccatac
      181 tttttagggt ctataacctc agaatcttca tctaaaactt ttttaacccc tttactatat
      241 gcaattctta attcagttgc atagttaact tttgcaatac ctctttttat agcttctttt
      301 ataatatcat catgtattcc tgaacctcca tgcaatacta aaggtattga aactacattt
      361 ttaatttctg ataacctatc taaatctatt ttaggttcac ccttatacaa tccatgtgca
      421 gtacctatag caatagccaa tgaagatatt cctgttctat cgacaaactc ttttgcctct
      481 tttggatttg tataatcatt actattcccg ccatctaaat catcttcttt tccaccaact
      541 ttcccaagtt ctgcttccac tggtataaga gaaggactac atgcttttac tactgattta
      601 gatatagata tattattttc aaagctttca tgagaaccat ctatcattat tgatgtatat
      661 ccacttctta aagctttcat tgcaaggtca aaacttgaac catggtccag atgcattgct
      721 acaggtatct ttactctttc tgcagcaacc tttgcatttg ctagataata atcaagacct
      781 gcatatttta cagtagatgg agtagtttgt aatattactg gagagtttaa ctcctcagct
      841 gcttctataa cagcaattac catttccata ttttctacat taaatgctcc tactgcatac
      901 ccctcatctt gtgccttttt aagcattgat gttgttgtta ctaatgccac attgataccc
      961 tcccaaatta tttagttata atattttaat cttatctttt aatattcata tattgttaca
     1021 ccttttacta ctctatttac tgttcctgta gctgatggcg tatctggctt atttccaacc
     1081 tttaccgata caaacaatgc tatagtttgt gcaaacaata tgtatggcat tgcaagatac
     1141 acatctggta atttattata cttttcttta aactcaaact ttgttcctga gaagttattt
     1201 tcattttcta ctgacactgc acaagtgaat ttagcaattt tatccctttt tatctcctct
     1261 aaaacatcta aatcatattt tcttgtatat aaacagttag atacaaattc aaatacaagt
     1321 gtattttcat caataaatga ttttggtccg tgtctaaacc ccataggaga atcatacacc
     1381 gtagatattt ttcctgctgt aagttctaaa agttttagtt gtgcttcttg tgtcaaacca
     1441 cctaatcctc ctgaacctaa atatacaact ctatcgaaat ctttatttat taactcatgt
     1501 atttcatctt ttctatctat aacatttctt cccatttcaa ttattgcatt tatatatgac
     1561 ttttctacat catcttctaa actatcgaat attaacatag cagataacat catacacgaa
     1621 aaacttcctg tcatagcaaa gccttcatca tttgattttg atggcattaa taacaagtaa
     1681 ttattttcgt cgtttttagt cattttagct agttctcctt gcggattaca agttatagct
     1741 aaatgataaa aattatctac aatcttattc cctaaattta aagcagccaa actttcagga
     1801 ctatttccac ttcttgcaaa tgaaattaat aatgttggta tatctttttt taagtaatca
     1861 tatgggtttg aaactatatc cgtagtaggt attgcttcaa atatgtattt tcttatgtca
     1921 tttttattct taaggtaagg aagtattgaa tttcctatgt aagctgaagt tccagcacct
     1981 gtaaatataa ctctaaactg cccatgatta ttacttattg tatctatgaa tctatttaat
     2041 ttttctttgt tagatttata tatttcataa gtctctaacc ataactcagg ttgttgtttt
     2101 atttcattag ttgttattat tgcacctaaa tcttttagtt tttgttcttc taatttaaac
     2161 attttcattc tcctttcata cttgtaacgt ttacatttgg taat
//
LOCUS       DACTGW010000350         2159 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.357, whole genome shotgun sequence.
ACCESSION   DACTGW010000350 DACTGW010000000
VERSION     DACTGW010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.357"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            80..1249
                     /locus_tag="I9573_003578"
     CDS             80..1249
                     /locus_tag="I9573_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAT4798161.1"
                     /translation="MLNIRTLCHEINDWIINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKNYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            1350..1826
                     /locus_tag="I9573_003579"
     CDS             1350..1826
                     /locus_tag="I9573_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAT4798162.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPNVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
BASE COUNT          790 a          303 c          320 g          746 t
ORIGIN      
        1 tgatacataa taatgattta cataaaataa tataattaat gaagaaaaat aaatttttaa
       61 tatagttagg atgtgtttta tgcttaatat aagaactctt tgtcatgaga taaatgattg
      121 gattataaat ataagaagag atttacataa gactccagaa cttggattag aagaatttca
      181 aactaagaaa aaaattatta aatacttaaa tgaaataggt ataaattata ttgaatataa
      241 gaatcataca gggataactg catacattaa cgtgagtcca aactttgaaa cagttgctat
      301 aagagctgat atagatgcct tacctataac agaagaactt aattattctt ataaatctat
      361 taatattggt aaaatgcatg catgtggtca tgatgcacac actgctattt tactgggtac
      421 ttgtaatata ctatttaaat taaagaatta cttaaatgta aatgtaaaat tcttttttca
      481 acctgccgag gaaactattg gtggggctca acttatgata gaagatggat gtcttgaaaa
      541 cccaaatgta aaatatatat ttggactcca tgtaaatccc aatatcaaca aaaatctaat
      601 tgaattaaaa tacaacactc taaatgcttc caccgataca cttcaattaa ctgtacatgg
      661 ttcaaaatgt catggagctt atccacatca aggtattgat gctattgtta tatctgctca
      721 tattattaca gctctacaga caatcgttag tcgaaacaca aatccaactg attctgtggt
      781 tatttctcta ggaaagattg aaggtggaat caaggaaaat atagtttgtg acaaagtggt
      841 tataaggggt actttgagaa ccttgactcc tgaaactaga gagttttcaa aaaaacgaat
      901 tagagaaatt tgcgatttta cttgtaaaac atttggagga agtatttctg ttgaaattga
      961 ggaaggttat ccagctctta tcaattcaaa ccatctagta gattacgtca agcaaaatgc
     1021 tgtagaattg tttggagaag aaaatattat ccttaaagac tcgcccactc ttggggctga
     1081 agacttttcg tattttctaa gacactgtga aggagctttt taccaccttg gttgtgcaaa
     1141 tcgtgaaaaa aacattactt cccctttgca tacctctact tttgatattg acgaagattg
     1201 ccttattact ggtgtcatgc tccatgtaaa aaatgtatta tcattctaac tattaatatt
     1261 tttatttatt attttatatt gaccctcaca tgatgtcatg ttatacccta aaattacaaa
     1321 tgataaatta aatagttgga ggtaatatta tgaaagaaat attaataaat cctataggta
     1381 aaatatgtat tcaaggcgaa gaggtcttta ttaaattaga caaaaagtat gtacctgcat
     1441 taaatgaact cgacgatttc agtcatttaa atgtattttg gtgggctgat gggtttgata
     1501 atcctgaact aagaagtatt ttagaaaccc caaaacctta taaaagtgga ccaaacgtta
     1561 taggaatatt tgcaacaagg tcacctataa gacccaatcc aatagcattg actgctgtac
     1621 aaattatcaa tatcgaccat gaaaatggta taataaaaat accatatatt gatgctaatg
     1681 ataatagtcc tgttatagac ttaaagccat atactccaag cgttgatagg gttgaaaatc
     1741 cgtccgttcc taaatggtgt agtcactggc ctaagagtgt agaaaaatca ggagaattta
     1801 attgggaaga tgaaattgaa ttttaaatta cttttttctt gatttcatta atttgttttc
     1861 cacatttatt ttatttacac ttaccaagat tttatgtcac atatacatat aatattaggc
     1921 tgtattacat aatacagcct aatattatat aataaatttc tatttttccc ttaaatcatt
     1981 cacattcttt ctaagctaca taagattttt ctattatttc tttttgagtt taatttagca
     2041 ttatgttgag gaaaaaacat ttcatgcatt ttaataaatt aaatttttct tttattctac
     2101 gattttataa taatcaattt aacttaaaaa taatacctaa ctattttaac tatgttaaa
//
LOCUS       DACTGW010000351         2155 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.358, whole genome shotgun sequence.
ACCESSION   DACTGW010000351 DACTGW010000000
VERSION     DACTGW010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.358"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..414)
                     /locus_tag="I9573_003580"
     CDS             complement(<1..414)
                     /locus_tag="I9573_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4798163.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKID"
     gene            713..922
                     /locus_tag="I9573_003581"
     CDS             713..922
                     /locus_tag="I9573_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4798164.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            954..1163
                     /locus_tag="I9573_003582"
     CDS             954..1163
                     /locus_tag="I9573_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798165.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(1380..1778)
                     /locus_tag="I9573_003583"
     CDS             complement(1380..1778)
                     /locus_tag="I9573_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4798166.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMMILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
BASE COUNT          804 a          243 c          276 g          832 t
ORIGIN      
        1 atctattttc tcatctttat ttttgttata ctcaattttt cctaaatcct tctctttttc
       61 atattcatga agttttaatt caatcatacc ttctattttg gctttatcat aatcatttaa
      121 ctttctaaag ttgtttaaaa gctttatttc attagagttt atgctattaa gtggatagtt
      181 tgaggaggaa tcgcaaatta aatctgattt atgtgataga ttatctccat ttaatagcca
      241 gtctactgaa acattaaata tctcagctat agattttaat atttcataat ttggttttct
      301 aatgtttctc tcaaatttac ttaagttgtc acagcctaac atttcttcta gttcatattg
      361 tttaaggttt tttgcttttc tcaaataaac aattctttct cctaaagtat ccataaacac
      421 tctccattca attaatgtca aaaagacttt ttaagatgta aatagtttca aattaaaggt
      481 caaaatgaca taaaaaccat tgacttaagg tcaaaatgac tttataatta acttaatgat
      541 acgaatttac atcctaattt tagcacaaag taatcaaaaa atcttattta gtattaaata
      601 aatttatata cttaatatgt gtacatatta aaaatatata ctaaatagag ggggtgcgta
      661 agctaaagta atataaaagt aaatataaat cacttagaaa ggaagttgat aaatggatgc
      721 tcgaaaaaaa tggatacctt ttttgggagt gcaagtcaag caaagactta ttgaattaaa
      781 tatgactcaa agggaattag cgaagaaaat aggtgttaat gaaaactatt tgtcagctat
      841 tttaaatgga agaagaacag gtaaaaaata taaatcatca atttatcaat tacttaatat
      901 agaatattca gaagatgatt aataaatagt atataaagta ggtgaatatt cttgtgtgca
      961 aattggattc agatggggtt atagagtgtt gtagagcaat tgatgatttt attacagcac
     1021 ttagtaatat aaaaagctta aatatggaaa gattaaatac tttaactaaa tattctagta
     1081 catgttcaat ccttcttaaa gaggggaatt atgaaggatg tacaattgtg tatagaaaga
     1141 tgttggaaga attaaaaaca tgagtaatgc atttcttagg aatataaatt atacatagaa
     1201 atgtattata tttttcaaag tacttaaact aaaatatgga taagataatc taaatattat
     1261 aaatgtgctt gaaattagac tatacttgtt tttaaataat ccaatatcca tattttagta
     1321 atatactaca aaaaaagaag gttaatagat gatgtaaaat cgtatcaaat tatgtatgtt
     1381 taaaccattt tatcttcatt attattagag gaatgctttt ttaagtcttt atattcagat
     1441 atcttaagtt caagtattcc ttctattttt attttatcac gttcgtttag ttgtctgtat
     1501 agatttaata tcatcatttc atcattagta acatgtaagt aatcttcttt atcttctttt
     1561 acactactat tgacatttac cttctcttta ccatagagaa gccagtcagt cgtaacatta
     1621 aaataatcag ctattgacat tagtatatca caattaggtt ttctatctcc tgtttcatac
     1681 ttgcctaagt tttcaaattt taaaatatcc ataagtttgc gctgagtaag ttttttggag
     1741 tttctcaaat aagcaattct ttttcctaaa gtatccacaa aatacactcc tttcttttta
     1801 tgagtaatgt ctaaatgaca tttgaaatta aaaatatata aatttataat ataaaactac
     1861 taaattaaag tctaaatgac attttgctta aattaatatg ctcataatat gattttaaca
     1921 tattatagtt gaaaatatat ggtttatttt gatttgtata tataacaata gatttaattg
     1981 ttataaaaat gtaaaggggt gtatgaatag attgtataaa tttatttcga taaactaaga
     2041 ttgctttttg attgtctgta aaagagaaaa agattaagat aaaaatagta ttatattgta
     2101 atttatatta atcaattaca aagattttat gaatttattc tttagggtaa aatat
//
LOCUS       DACTGW010000352         2153 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.359, whole genome shotgun sequence.
ACCESSION   DACTGW010000352 DACTGW010000000
VERSION     DACTGW010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.359"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(88..1443)
                     /locus_tag="I9573_003584"
     CDS             complement(88..1443)
                     /locus_tag="I9573_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAT4798167.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT          787 a          379 c          235 g          752 t
ORIGIN      
        1 tatttttatt attaattagt tataaatcaa tctaatacaa cataattaca gtagattaat
       61 ctataaaaca taataattac ttactgatta tttatcatcc tcaacaagtc tacttaatgt
      121 cttatcagat aagaataata atatcgctgc aacaaatagt attactggaa ttatagtata
      181 tactttaatc atatcaaatt taagagtaaa tgcatatata tatccatatc ctttaccagc
      241 tataaaagta gcaaatgtcc atactcccat aagaacacct agaagcttct taggtgcata
      301 cttacttaca aaagagttac caagtggtga gaataacatt tcaccaagac ttaaaagaat
      361 accaaacatg attatccata gtatactagc tttagcagtt tcaggagctc caactcctct
      421 tgaaaattct gcaccaacaa gcattaagaa tgcaagacct aagaatatta atccaagacc
      481 tgtcttttta aataaactca tatctccttg tggtctacta gcaagtttaa accataatgc
      541 tccaagaact ggtcctaata ctatacatac aagtgaattt aatgagtcaa accaagcaag
      601 aggaacatca aatcctccaa cattcatgtt tacaaatgct gcaccataat cataaactgc
      661 aagataagtt agataccaga atacccagaa aattacagag aagaatgata taagtataat
      721 agcccaaatt ctcttcttat ccatgcttgt aagaggagac ttatcttttt cttcaacttt
      781 ttcagcaact acaccttctt taaaaggtct ctttcctgca tttccaagga atctccatcc
      841 atatatgaac catattgcac ctattacaca aagtacagct gctataaaga aacattgaga
      901 aaatccaagt acaccatttt ttgcaaatgt tttaaggtaa agaataccaa ctgctatagt
      961 accaataaaa gaacctatat taacaaaaga atattgtact gaaaatgctg tatcaagttc
     1021 ttcttgacta tcaaaaagct gtccatttac cgcagatacg ttacctttaa agaatgcagt
     1081 accaatcgaa actaagatta tcattgcatt taccatagaa actgtagttg caatagaacc
     1141 aagatagtaa cctaccccca tgataagcat acctataggt atagtatatc tagctcctat
     1201 ccatctatct gatatatatc ccccaattat aggtgcaagg tatgtgaatg caacaaggtt
     1261 ggcttgcatt atagcagctt gtcctttatc tattccaaga ccaccatgaa ctatagctgt
     1321 tgtaaggaat atatatatta agtactttga agcataatat gctgctcttt caaatgagaa
     1381 agttgttgag cagatataaa atcccatagg aaatttaccc ttctttttta ctgttgagct
     1441 cataatttta aatcctttct taattgtatt tttatttttt acgttttgtc caaattcttt
     1501 cttttatgaa agatataata ttattatttt gtaaaacaat ttgtaattat tattaagata
     1561 attgcgcatt tatttgtaga gtacatattc tcatggaata aaattaatac acttcttaat
     1621 cataaattta aattatataa attcactatt ttcagatata gttataaatt aatatattct
     1681 catctaatac catgaaaaaa ccttcgccat atgcaattct tattttttac gtgatagtat
     1741 tttacaaact aaaatccccc ttttgtagca acttctgtaa ttacatttta gtgctttttt
     1801 aaaggagtct cgaataaagc aacatatatt ttgttcaaat aatgttttta tttacttttt
     1861 aaatttatat attacttcac tcaaccaatt ctcaacttaa ataaataaac tactatctat
     1921 tcacagatta gacctatttc ttacacatta gtttataata aacactgtga atctaaacat
     1981 aaaaactgtt attttaaagt aattacccat atattcgctt tgatactatt tattgaggtt
     2041 atactcttta tttataatat attatttata aaatacctca aaatatatta ttaataaaat
     2101 acctcaaact gaaaactttt tcacgaatat ttatttttca cctccccttt ttt
//
LOCUS       DACTGW010000353         2081 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.360, whole genome shotgun sequence.
ACCESSION   DACTGW010000353 DACTGW010000000
VERSION     DACTGW010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.360"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..295
                     /locus_tag="I9573_003585"
     CDS             <1..295
                     /locus_tag="I9573_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAT4798168.1"
                     /translation="EVDYLEKLIRNQNIKIDYITLSHIYETKCKEGLNPKGIELLKEA
                     KKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIKKTISNYNEKLN"
     gene            483..1877
                     /gene="yfcC"
                     /locus_tag="I9573_003586"
     CDS             483..1877
                     /gene="yfcC"
                     /locus_tag="I9573_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAT4798169.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT          729 a          248 c          364 g          740 t
ORIGIN      
        1 ggaagtagat tatttagaaa aattaataag aaatcaaaat ataaaaatag attacataac
       61 attatcacat atatatgaaa ctaaatgtaa agaaggatta aatccaaaag gaattgagct
      121 tttaaaagaa gctaaaaaga ttactgatat taaaataatt gcacttgggg gaattttacc
      181 atctaatgtc aaagaaacat taaaatactg cgatgatttt gctataatgt caactataat
      241 gaggtctaaa gatattaaaa aaaccatatc aaattataat gaaaagttga attagtaaat
      301 atagtatatt gaatttttaa cttaaataaa aataaaaaag ttatctttta aattttaaaa
      361 ggtagctttt ttattttata tctttgctaa taaaaaattg taaaaaggga aaactttagt
      421 cagagagatt aaacaatatc ctagaaagat ttaaaattta gtaaagtatg gaggacatta
      481 taatgactga cagtaaaaag aaacgattta ttataccaca cacttttaca ataatatttt
      541 tactaatagt cttgatggca atattgactt ggatagtacc aagtggtgaa tttagtagag
      601 aagatgtaga aggtagaatg gtagttgtac cagggactta tcaaagtgta gaaagtaatc
      661 ctcaaggatt tgcagatgtt tttaaagcac caatagaagg ctttatagat tcagctgaag
      721 ttgtaggttt tgttttaata gttggaggtg cttttggtat tgtaaatagg actggagcta
      781 tagaagcagg aatagcttcg gttatctcta agtttaaggg aaaagaactt cttataattc
      841 caatatcaat gattttattt ggacttggag gtacaacatt tgggatggca gaagagacat
      901 taccatttta tatgatattt gttccactca tgacatcctt gggatatgat tctctaacag
      961 caatagcaat tgtatttata ggagcagcag caggagccgc cgcttcaaca ataaatccat
     1021 tttcagttgg tattgcacag gcactagctc aacttgctcc tggttcagga gtaacttaca
     1081 gagtagttat ttttatagca tatatactaa taagtatagc atttgttatg tactatgcta
     1141 agaaggtaaa ggaaaatcct gaaagttcaa tagtatatga aatagataaa aataataaat
     1201 caatacttaa tcaaacttca acagaaataa aagaatttac ttttaaagaa gtatctgttc
     1261 ttgctatatt tggaataggg atggcaataa tgatttgggg tgttttaagt caaggatggt
     1321 acattccaga aatatcaatg tgttttgctg caataggtgt gctttctgga ttggttggta
     1381 gattatctca aattgaaatc tcagaaagtt ttatagatgg agcaaaagat ttagcatctg
     1441 cagctcttgc tatagctttg gctcgtggaa tcgtaataat tgctcaaaat ggatttataa
     1501 tagatactat attaaattcc gcagcagcct ttctaggtgg gcttccaaag gttatatttg
     1561 tatatttatc atttttcttg gaagctgcat tggcattttt aattccatca tcttcaggtc
     1621 ttgcttcact tacagtacct gtacttgctc ctcttggtga tttggttaat atatcggctc
     1681 aagtgatagt aaccgcttat caatttggtg tgggtcttac aaactttata actccaactt
     1741 caggagtact gatgggagca cttgcagtag cccatattcc attttcaaaa tgggtaagat
     1801 ttataatgcc tatcttgttg attttgacag ctgtatctct agtcttttta acaataggta
     1861 tttatatagg gttttaatta agtgtagttt tatttaagat atttagtaat ggtataaaat
     1921 gataatatgt tttcaataaa ttgccactaa tttaattagt ggtaatttat tttttgtatt
     1981 taaaaataaa tgtatttact tgtattttga attaatatct acttagacat tgttttaatt
     2041 gttatatttt catttaatta agcattgatt tatcgttttt t
//
LOCUS       DACTGW010000354         2072 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.361, whole genome shotgun sequence.
ACCESSION   DACTGW010000354 DACTGW010000000
VERSION     DACTGW010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.361"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            56..400
                     /locus_tag="I9573_003587"
     CDS             56..400
                     /locus_tag="I9573_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAT4798170.1"
                     /translation="MNNKEKGDFGEEIAVNYLLSKGAKILEKNYRLRIGEVDIIANIG
                     DEVVFVEVKSRSNIKYGYPCESVDFKKRKKIIGVAKYYIIKNNLNNTPIRFDIIEVYL
                     LEKRINHIMNAF"
     gene            431..2032
                     /locus_tag="I9573_003588"
     CDS             431..2032
                     /locus_tag="I9573_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HAT4798171.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLLVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVSIMSFLDDKSRKENGELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINESKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
BASE COUNT          894 a          179 c          388 g          611 t
ORIGIN      
        1 aaattgtata ctgacgataa tttaaaagta aaaatttaat atattggagt agataatgaa
       61 taataaagaa aaaggtgatt ttggagaaga aatagcagtt aattatctgc tatcaaaagg
      121 tgcaaagata ctagaaaaaa attatagatt aaggataggg gaggtagata taatagcaaa
      181 tataggagat gaagttgtat ttgtggaggt gaaatcaagg tctaacataa agtatggcta
      241 cccttgtgaa tctgtagatt ttaaaaaaag aaaaaagata attggtgtcg caaaatacta
      301 tattataaaa aataacttaa ataatactcc cataagattt gacatcatag aggtctattt
      361 gttagaaaaa agaataaatc acataatgaa tgctttttaa aaaagataat gtttttggaa
      421 aggattaaat atgttaagta ttattaattc aagcaactta gtaggaatcg acagcttttt
      481 agttaaggtt gaagtagatg taagcaatgg aatacctagt tttaatattg ttggtcttcc
      541 aggcaaagaa attaaagaag caagagaaag agtaaagtca gcaattctta atagtggata
      601 taaatttcca agcacgagaa tagttgtaaa tttatcacca gcagatataa aaaaagaagg
      661 agctttttta gatttatcta taagtatagg ccttttaaga gagttaataa aaaaagatga
      721 aaattacata agagaaagta tgtttatagg agaattatca ttagatggaa aaataagaaa
      781 agtaagaggg atattgccca taattatggg tgcaaaaact cagaacataa aacgtatttt
      841 tataccaatt gagaacataa aagaaagtct attagtagat gaaattgata taattcctat
      901 taaaagttta aaagaatgtg tcgatttttt aaacgaagag ataaaagtcg ataaagtaag
      961 tataatgtct tttttggatg ataagtcaag aaaagagaac ggagaattag aaaaagataa
     1021 tagttatata gattgcaaat atactaagat taataatgaa gaaagtaaat atgatgaaga
     1081 ctttaaagat gtaaaaggaa attattttgt aaagaggagt gcagaaattg cagcagctgg
     1141 aaatcataat atgtttatga ttggacctcc aggctcagga aaaactatga ttgcaaaaag
     1201 agtaaggaca attttaccag atataagtat agaagaaatg atagaagtaa gtaaggttta
     1261 tagtatattg ggtatgataa atgaaagtaa aggaataatt gataaaagac cttttagagc
     1321 acctcatcat acaactacaa agcaatcttt aattggagga ggaatggatg caagacctgg
     1381 agaaattgct ttagcacata gaggtatctt atttttagat gagatagccg aatttgatag
     1441 aaaaatactt gagactttaa gacaacctat agaagatgga tatgtaaata tatccagagt
     1501 aaagtacagt gcaaaatatc cttgtagagt actgttagtt gcagctatga atccatgtcc
     1561 atgtggttat tatatgtcag agacagagtg tagatgtaga agtaacgaaa ttgatagata
     1621 cataaataaa atatcaggtc cattgttaga taggtttgat atttttgtag aagttaattc
     1681 tataaaatat tcagatttta attctttaaa acaagaagaa agctctcaaa aaataaagag
     1741 aagagtagaa aatgctagaa aaatacagat aaatagattt aagaaagata atataaaaaa
     1801 taatagtgaa attaaagctt acaatttatt taaatattgt aaactggaaa aagaagcttc
     1861 aaaaacggct gagatgatat ttaataaata caacctaagc agtagaagtt atacaaaact
     1921 tttaaaaatg gcaagaacaa tagcagattt agaagaaaga gacttgataa attcacagtg
     1981 tattatagaa gctttttcat ttagaaaggc ttattatagt tattttaaat agttttttga
     2041 ggtggatttt atggaaaaaa gagatactta tt
//
LOCUS       DACTGW010000355         2038 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.362, whole genome shotgun sequence.
ACCESSION   DACTGW010000355 DACTGW010000000
VERSION     DACTGW010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.362"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            16..873
                     /locus_tag="I9573_003589"
     CDS             16..873
                     /locus_tag="I9573_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAT4798172.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            970..1839
                     /locus_tag="I9573_003590"
     CDS             970..1839
                     /locus_tag="I9573_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAT4798173.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT          849 a          202 c          365 g          622 t
ORIGIN      
        1 atgggggtta gatttatgag taaagatgta aaggtaaagc gtaataagaa atatgataca
       61 tttagtataa ctatggaact tgagagacct ttactttatg atgaggttga gaacaaagat
      121 gataaaaata ataatagaaa atttaacata aagaataaga tatttaaggg aataggtata
      181 ctatttgtat tgattttgct tggctttttc atatggataa ataaaacata tgaacctcaa
      241 aagctggcaa aagaagctct agtcagcaat agtaaagttg aagtaactgt aaatgaaaac
      301 atttcattta caccaaaagg aagaaaagtt tctaaaggtt taattttgta tccaggagct
      361 aaagtagaaa tagaatctta tgctccactt gcaagaaaaa tagcagaaag tggatatgag
      421 gttgtaatcg taaagatgcc acttaatcta gctatacttg gaactaataa ggcacagaaa
      481 gttatggatt cttacaacaa catagaacat tgggtaatag gaggtcattc attaggagga
      541 gttgcagcat caaattttgc acgtgataat aaattaattg aaggcgttgt atttttagct
      601 tcttatccaa tgggtgatga gttgaaagaa ttaggaaaaa aagttatatc tatatggggg
      661 agcaaagatg gagtagtcaa ttttaaaagt cttgtagaat caaaacaaaa acttcctgat
      721 gatacaactt atgttgagat tgaaggagga aatcatgcac aatttggaga ctatggaaaa
      781 caaaagggag ataatgatgc tattataagt caagaaaaac aacttaatat tactgccaat
      841 agtatcatta aatttttaaa gaatatatct taaactaaaa tttaaaaaat ataattttat
      901 aagttattat ttaaagtaaa tattaataat ataatagaat gaatatttta ttacaagggg
      961 gattttaata tggaatacga tatgccactt tacagaccac caagtgaggc ttatagtctt
     1021 ataatacaag tcacactagg atgttctcat aacaaatgta cgttttgtag tatgtacaaa
     1081 tctaaaaaat ttaaaataaa gccactagaa gtaataaaaa atgaaattga tatatttaga
     1141 agatattata agcatgtaga aagaatattc ttagcagatg gagatgcatt gataatacca
     1201 atggagaaat taagagatat tattttatat ataaaagaag tttttccaga gtgtgaaaga
     1261 attactatgt acggctctcc taaatctata gagaaaaaat ctgatgatga acttaaagaa
     1321 ttaagaaaac ttggtgttaa aatgatttat ctagggcttg aaagtggaag caatgaagta
     1381 ttagaagata ttaagaaagg ttttagtagt gaacaattaa taaaagttgg tagaaaagtt
     1441 aaaaaaagtg ggattaaatt atcagccact gtaatagcag gtcttggagg aactaaaaaa
     1501 acacatcagc atgcagttga tacaggaaaa atgttaggag caatttcacc agattatgtg
     1561 ggtgtactta gtttaatggt agaacctaat actgaacttt acgatttact taaggcagga
     1621 gagtttacaa tactagagga taaggctgtg ttacaagaaa ttaaagagat gataaagaat
     1681 atagatgcta atgaaaaaat tgtatttaga agtaatcatg catcaaatta tgctaattta
     1741 aaaggtgtat tacctgatga taaacaacgt ctaattgatg aaattgatta ctatctaagt
     1801 aaccaaaagc taaaaagaga agaatataga agactatagt ttttgatata tcaatctata
     1861 ttgataggta aatactaaga ttatttagat acaaaaaatc atgctacatt aatagttgct
     1921 tactactgat gtagcatgat ttttttatta aaaaacaaaa tggtatattc atatataata
     1981 tatcaataat aatgaaaatg tgaactagtt taatagaatt ttacctatta taataaaa
//
LOCUS       DACTGW010000356         1908 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.363, whole genome shotgun sequence.
ACCESSION   DACTGW010000356 DACTGW010000000
VERSION     DACTGW010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.363"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            315..1364
                     /locus_tag="I9573_003591"
     CDS             315..1364
                     /locus_tag="I9573_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAT4798174.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWVQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            complement(1514..1861)
                     /locus_tag="I9573_003592"
     CDS             complement(1514..1861)
                     /locus_tag="I9573_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAT4798175.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
BASE COUNT          716 a          248 c          285 g          659 t
ORIGIN      
        1 ttttatataa aataataaaa gtttctataa acaaattttt taataatata aatgaatatg
       61 atttatatta ttaaaaaatt tttccttata tataatagac aaaaaagtga tattatttta
      121 attttattac ttagtttata aataatcaat ataatttatc caatattaca tattactaaa
      181 aaaactaaac ataaaactga aaattattat taagtttttg tagaaatatt ataagcatag
      241 atacattttt gtaacaacta caataaattt ttaatactat atactattcg tatgtcttat
      301 gggaggtatt tattatgttt aaaaaaatag tcgcaatagc aagctctatc attgtaagta
      361 caatatgtct aactggatgt gattttaact ctaatgacaa aaaagatgaa tcttcttcgt
      421 ctaaaaatct ggataaagta actatagcag aagttactca ctctgttttt tatgctccac
      481 agtatgctgc tattacgaaa ggattctttg aagatgaagg tataaagatt gaccttataa
      541 atacccaagg tgcagataaa actatggcag ctttgatttc aggtgaagca aacgtaggtt
      601 taatgggtcc tgaagcatct atatatgtat ataatcaaaa caaaaacgat tatgcagtga
      661 actttgctca attgacaaaa agagatggta gttttctagt tgctaggaaa aaaatgccta
      721 atttctctta caatgattta aaaggaacag aaatattagg tggtagaaaa ggtggagttc
      781 cactaatgac ttttgaatat gttctaaaag aaaaaggtct tactattgga gaaaatctaa
      841 aagcaggcaa tgttaatgta agaactgatg tacaatttgg tgtaatggct ggagcttttg
      901 caggtggaga gggtgacttt acaacagcat ttgaacctac agcaacagca atggaaaaag
      961 aaggtagtgg atatatagtt acatctatag gaaaagattc tggagaaata ccttttacag
     1021 catactctac tacgagatca tacatgaaaa acaataaaga tttaattcaa agatttacaa
     1081 atgctgttta taaaggtcaa ttatgggttc aacaagcatc tagcaaagaa gtagctaagg
     1141 ctatgcaacc tttctttgaa gatatatctt tagaagattt aacaacagtt gtagatagat
     1201 ataaatcaat agatgcatgg tctcaaaatc ctgtattaaa ggaggaaggt ttgaagaaat
     1261 tgatgactgt tatgaaagaa gctaaagaac tagataagga agcaccttat aaagatatcg
     1321 taaatactga gtttgcaaac aatgctataa aaaatattaa ataaaatata ttagttttag
     1381 ctagatattt tcttgttttt ataatgaaaa ttaacaaaaa tataaaaaga gccttttaaa
     1441 agactctttt tatatttgag attaaagttt atataataaa atagatatta aagtactaac
     1501 tgtacttata tatctatcct ctgtattctg ctccagcatt aatttttgta atttgaacgg
     1561 ctaccatttc ttcattgctt ccattagtaa tttcatgcca ctctcctgtt ggtacaaata
     1621 cagtatcgcc gttttttaca tcacattcat gctcaccaag tttcatttta ccttctcctt
     1681 ttaggaacac aaatatttgt tcaccattag ggtgtctatg agcattaaga acttgattag
     1741 gtttaaaata ataaacttct gaagttatat cgttaccagc aaatactggt atttttggaa
     1801 ctgctgtatc attatattga gctaagcttc cgtctaatag attcttttta ttttcatgca
     1861 tactagaaat acctcttttc tatattatag atttactatt attatacc
//
LOCUS       DACTGW010000357         1893 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.364, whole genome shotgun sequence.
ACCESSION   DACTGW010000357 DACTGW010000000
VERSION     DACTGW010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.364"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            171..1382
                     /locus_tag="I9573_003593"
     CDS             171..1382
                     /locus_tag="I9573_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAT4798176.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            1574..1834
                     /locus_tag="I9573_003594"
     CDS             1574..1834
                     /locus_tag="I9573_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAT4798177.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
BASE COUNT          802 a          168 c          334 g          589 t
ORIGIN      
        1 agttagccat ataaaaaata ttaaaaatat atacttttca atattttttt gataaaataa
       61 gaatgaattt gtgcaatttt atttttctcc aataactaaa aatagttttt tgaattaata
      121 tttgcttaag aataaaaatc agaaacgttt ttgatatagt aggtgataat atgaaaggat
      181 tagttttaga aggtggagga acaaaaggag cctaccaaat aggtgcatat aaagctttaa
      241 gagagcttgg tatagagttt caaggagtaa ctggcacatc aattggtgct ttaaatggag
      301 catatataat tcaaaatgat attgaagtaa tggaagatat ttggttaaat tatgactata
      361 ctcattttat gaatattgat agagaaacct atgagaggtt taaaaatgta gatttcacta
      421 ctaaaaatct caatgcagtg tttgagctta taaataaagc tagaaaaaat gaaggtatag
      481 atataagccc attaagacaa ctaatggaga caacattaaa tgaagacata ataaggaaat
      541 caaaaaaaga ctttggactt gttacggtct ggtgggataa aaaaattaat ccatatccat
      601 tgtatatcga agatatacct aaaggaagat tgattgatta tttaattgct agttccagtt
      661 tgcctatttt tcaaacgaac atgatggatg ataagttgta tcttgatgga atgttttgtg
      721 ataacatgcc aataggactc ttggaagaca aaggttatga agatgttgtt gtaattaggt
      781 tgattgatga ttttattggg aaaatgaatc taaatcgaca tcaaaatata aatataaaaa
      841 ctataatacc atcagaatat ctaggaggtt ctcttaataa agatagagat aatgtagaaa
      901 aaaacatccg actaggttac ttagatacta tgaaagcata tgaaagatgc actggaatta
      961 agtattattt tgatgtagag tataaatata atgaagaata ttgttttgat aaatttagaa
     1021 gtatgagcaa tgaaacaata caatatatct gtaatttatt gaatataaaa agagatgctt
     1081 cacttagaac tgttatggaa agtgtaatac caaagcttgg tgaggtgctt ggattgccaa
     1141 aggacttttc atataaagat ttgttttact gtatatatga aaagaaactt gaagaaaata
     1201 atataaacag gataaaatta tatgatttta acaaggttat agatgttgta aatacaaata
     1261 ttgattctaa tagaaattta gcttctaatt ttgaagttag acctaaaatt agtattaaaa
     1321 agaacaaaaa cacaaaatta ttgacaaatt gtattataaa agacataaaa aatcaaagtt
     1381 aaataaaaaa caaaaaaaaa tttgtaatta tactcgaaaa aagtgtatat tagtagtata
     1441 atatttttgt agcactagtg ggaacaaaaa atgaccatag gacaaaaaag taccacttag
     1501 taccattaaa gtaaaaaaca ggaaaacaat tttaaaaaat ataaaatgta aagtttatag
     1561 gaggatttaa aaaatggaaa aagtagttag tatcaaaaat gcaagtggat tacatgctag
     1621 accagcagga atgtttgtta agaaggcagc tgaatttaaa tcaacagtag aggttgtagc
     1681 aaaagacaaa actgtaaatg caaaatcaat aatgggaata atgagtttag gtttagctca
     1741 aggagaagaa ttaaagttag ttgtaaatgg agaagatgaa gaagtagctt tagctgcaat
     1801 ggttgaatta gttgaaagtg gatttggaga atagatataa taatttttta aaatttgata
     1861 tttagaatct aatattactt aaagtaaagt aac
//
LOCUS       DACTGW010000358         1881 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.365, whole genome shotgun sequence.
ACCESSION   DACTGW010000358 DACTGW010000000
VERSION     DACTGW010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.365"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            543..1010
                     /locus_tag="I9573_003595"
     CDS             543..1010
                     /locus_tag="I9573_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAT4798178.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDILGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKGIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEIGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          788 a          108 c          257 g          728 t
ORIGIN      
        1 aaaaataaat ataaatatca aaaataaatt tatttaaaat taaaatttta tttattatta
       61 aagagtaaat ataattatta gtaatatata tagtatttgt aataaaatat caataattag
      121 aaagattatg aaaactaaat tagaagaact aaaaactata tttttattaa ttataatatt
      181 tgaataatct ttttaatcat ataaaatgca tatacttgta atatttaaag gtgatattaa
      241 tattattagt gtatattaat attcccttga aattaaaaaa acatagatat ttcagtaatc
      301 taaaatagtc tacaaaaata acagttttag caaaattaat aagaatacat atagatttaa
      361 acctaaaagt atgaacatgt ctgtttttta caagattaaa atttatatta tatgataaaa
      421 ttaacttaac tatttcaata agacttataa tttttttctt tatactagac cttattgaaa
      481 cctttatagt ttaattctaa ttatatatta taaataaagt atgtgtatat taaggaggta
      541 atatgaagtt tttaaatgtt ttaatagtag ttgaagatat tgagaaatca aaaaagtttt
      601 attatgatat tttggggtta aaggtaatct gtgattttgg agaaaatgtt gtattagaag
      661 gtaatatatc attacaagaa aaaaagcttt ggttagaatt tattaataag agtgacagtg
      721 aagttaagtt caatggaaat gatgctgagt tatattttga ggaagataat tttgatactt
      781 ttgttgaaag attgagtaca atgaaaggta tagattatgt tcatctagct atagagcata
      841 ggtggggtca aagagcaatt agattttatg atttagatgg gcatattatt gagattggag
      901 aaactatgtc atctgtgtgt agacgttttt tagatagtgg tctttcaata gatgaggtag
      961 ctaaaagaat ggatgtaact gtcgaatata ttgaatcagt tttagaatag atggattata
     1021 ttaaagtaaa actatatttt gtatagaagt aagggctgta agtaaacttg tatggaaaca
     1081 aatttactta cagtttttgt ttttgtaaga tacagaatat aatatttgtt ataattttat
     1141 ttggattttt aaaaagtgca tgattttcct tacatattat aatagaattt aatatttatt
     1201 ggatgattta aatttagaaa aatataagta ttagcttata aaagataatt atacttacta
     1261 tcaagctgta gaatcatata tactatttta tgtaattatg ttaaaattta attgaacaaa
     1321 ttaaaaatat agtgaaaatt gtagttttag tttatttatt taataaatat ttcaaataaa
     1381 ataatgttta aataagaaac ataaataata atattattat tgttcttaaa gacaaaaaat
     1441 cttagcttaa aaatttgtat catataaaat ccctacatat tatggaagta atgatacatt
     1501 atatgatata cacaataaag tataaaagta tttacatgtt ttttctatat attttataag
     1561 ttttaggtat ggaaaattac taaataaaaa ttagattaat gattatgata gtatagataa
     1621 agtttattct ttttattaat agaaattgtt gtataaaaat aataaaagtg aggagagtga
     1681 tatttaaata aaaaaatttt aataagtatt ggtaatatag tttttaggga tgattatttt
     1741 atgtaaaaag ttgttatttg taaagataaa tatagaaaat attataagta ttgatttagt
     1801 gataatctaa aaggtgagaa ttgtaataaa aagagaacat atccttcaaa tggatgtaaa
     1861 cttataaata acattattac a
//
LOCUS       DACTGW010000359         1872 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.366, whole genome shotgun sequence.
ACCESSION   DACTGW010000359 DACTGW010000000
VERSION     DACTGW010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.366"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            86..700
                     /locus_tag="I9573_003596"
     CDS             86..700
                     /locus_tag="I9573_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAT4798179.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            718..1080
                     /locus_tag="I9573_003597"
     CDS             718..1080
                     /locus_tag="I9573_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4798180.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYKGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1186..1683
                     /locus_tag="I9573_003598"
     CDS             1186..1683
                     /locus_tag="I9573_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HAT4798181.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          710 a          213 c          319 g          630 t
ORIGIN      
        1 gtctttattt tgtctaaatt aaacaaactt ttttacaaat aaatacataa aatttaaact
       61 aatctattta ttggaggaga taaaattgga agaagtaatt gcaaaaatta ttactataga
      121 aaatggattc aaagaaattg aaaacattgc taaaaaaatt gtaaaaaata atgactcaaa
      181 aaagtgttat tatctatcaa tctacttata taggtcagaa tattatcaag taagagaatt
      241 ggctgtgttt atacttggtt ttatatcagt aactatttct gaagctttgt tatttttaaa
      301 agacaatgtg agtaaagatg aaaactggag agtacaagaa atactagcta aatcatttga
      361 ttattattgc agtgaagtag gatatgaaaa ggcacttcct gttataaaag aatggcttaa
      421 ttctgatagt ccaaacataa aaagggctgt gacagaaggt cttagaattt ggactagtag
      481 agactatttt aaatctaatc ctgacgttgc tatatcacta ctttctagtt taaaagaaga
      541 tgatagcgaa tacttgagaa agtctgtagg taatgctctt agagatataa gtaaaaaata
      601 tccagattta gtaaaaaaag aattagattc atgggatata tcaaataaaa aagttgagtt
      661 tgtatataag cttgcttcaa aatgtatctt ggcaaagtaa aatggaggtg aacttatttg
      721 aaacttcgtg atgagtatac ttgcccatta gaactaacac atgatatgat aaaaggcaaa
      781 tggaaatcta ttatattatg gagattacgc ataggaccaa cctccttatc aaaacttgaa
      841 aaggaaatca atggtattaa tcaaaagatg ttacttgaac aacttaaaga attaattgaa
      901 tttggtcttg tagataaaaa gacatataaa ggttacccac ttaaagttga atactcactt
      961 acacctattc gagggaaaca aatattggat gctttggcta taatgcaaga tataggaatt
     1021 agctttatgt tggataatgg tatggaagat attcttaagg aaaaaggtgt tttaaaataa
     1081 atagataccc acaaaaaagt aagtaattta ccattatttt caactggtat ataattaaat
     1141 tatagatata ttctattaat ttattataat aggaggtaaa ttattatgaa agtaactagt
     1201 acaggtatag taaatggtgt tatagaagat aaatacggta aaagaggaac tcagttcaat
     1261 gaaggtggta tgcctacata ttcactacct ctaaagtttg aagatgctcc aaagaataca
     1321 gtatcctttg ctgtattttt agaagataaa gactctgttc ctgtatgtgg gtttgcatgg
     1381 atacattggt tagctgtaaa tataacaaaa gatgagttaa atgaaaatga aagtgtaaca
     1441 gctactgatt ttgtacaagg gactactagt tggcatggca aactaggagg tcttgacaga
     1501 ttagaggcca gtgtatatgg tggaatggct cctccagaca gacctcatgt ctatgaaata
     1561 catgtatttg cacttgatac tatccttgac cttgaaaaag gattctatat gaatgaattt
     1621 ttcaaagcta ttgaagggca tgttttagat acatttacac taaaaggaag ctattctaat
     1681 taattaggtt tactatagct aatcattaat taaatgctac atttaattga tgattagctt
     1741 aattgttatt agaatatttt ctgcacttta tcttaatata tattttagtt gactggtatt
     1801 ttaatttcta ttagattatt atcaatttat ataaattgat tttagcttaa aacttactta
     1861 tctatttcag tt
//
LOCUS       DACTGW010000360         1832 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.367, whole genome shotgun sequence.
ACCESSION   DACTGW010000360 DACTGW010000000
VERSION     DACTGW010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.367"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            345..1673
                     /locus_tag="I9573_003599"
     CDS             345..1673
                     /locus_tag="I9573_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAT4798182.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGDKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGIKR"
BASE COUNT          636 a          207 c          334 g          655 t
ORIGIN      
        1 ttacagaaag aagccaaaat gtttttgaaa aaaatgaaat tttattgcaa aaaaatcaaa
       61 atcaatttaa cttaatagtt ataatctatt tttagtgtta caaatataca atgtatatta
      121 tacaatataa aaaatacgtt tatttataaa tattattata tttggatttt tggtttttat
      181 aattttttta tatttaaaca taactaaaag taaaagatta cgaaagctaa gtagagttta
      241 ttttatttta ataaataaaa aagctatttt tctatatatt ggcataatat atgcttattt
      301 aactatgaaa ttaaaattaa ataggattaa tgaggaggaa aagaatggga agtaatgaac
      361 tgaaaaagac tcttggcgta tcagcagcat tatcaactgt tgttggttct gttataggag
      421 cgggcgtatt ttttaaacca caagcagtct atacattaac tggaggagct ccaggtcttg
      481 gtatattggc atggttaata gctggaatca taactataac agctggtctt acagctgcag
      541 aagtatcagt tgcgatacca aaaactggtg gaatgatggt atatataaaa gaaatttatg
      601 gagacaaatt aggtttttta actggatgga tgcaaattgt attattttat ccagggatga
      661 tggcagcatt aggtgttata tttggagaac aagcatcagc actaattggt agcccatcac
      721 ttttacttcc aatagcaatt ggaattatag taatagttgc agggcttaat atgttaggtt
      781 caaaaactgg aggagtcata caaactgtct ctacaatttg taagttaata ccacttattt
      841 taataatggt tgttggattt attaaaggtg gtggaaataa tccaatactt acacctatgg
      901 ttggtgaagg attaagcctg ggtagtgtac taggtcaagt tttaattgct atattatttg
      961 cttttgatgg atggatgaat gttggtactt tagctggaga aatgaagaat cctggaaaag
     1021 accttccaaa agctataata ggtggattgt cagttgttat ggctgtttat tttataataa
     1081 atttagcgta tctttgggta ttaccagcta atgaattagc aaattatgct tctcctgcat
     1141 cagcagttgc tgaagttata tttggttcaa tgggtggaaa aataatatct gtaggtatat
     1201 taatttcagt gtttggagca ttaaatggat ttttactaac tggttcaagg gtggcatata
     1261 ctttagctac agataagact ttaccaaagt atggtatatt ttctaaatta aattctgctc
     1321 aagttccagc aaatgcaata gcattagtat ctgttattgc atctatatat gctttgtcag
     1381 gtcaatttaa tttattaact gacttagctg tatttgctac ttggatattc tatgtattaa
     1441 cttttatagg ggttatgaaa cttagaaaga ctcatccaaa tataccaaga gagtataaag
     1501 ttccattata tcctattgtt cctataatag ctatagcaag tggtatattt gttgtagtta
     1561 atcaattgtg ttttgcaggg atgaaaacta ctatgatatc tataggaggt cttgtaataa
     1621 cagctatagg cttaccagtg tatgcttata tgactagagg aataaaaaga taaaccaagt
     1681 agtttacaaa tgctttataa ttaaaataaa agagactgtc tcaaaataga gataagtctc
     1741 ttttttattg ttaaaagggt aaagagatgt tttattttat aaaaaaagag tgttctatga
     1801 actaaaaagt ttattttaag gtattcactt ta
//
LOCUS       DACTGW010000361         1824 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.368, whole genome shotgun sequence.
ACCESSION   DACTGW010000361 DACTGW010000000
VERSION     DACTGW010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.368"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            77..361
                     /locus_tag="I9573_003600"
     CDS             77..361
                     /locus_tag="I9573_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798183.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGRICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(1131..1346)
                     /locus_tag="I9573_003601"
     CDS             complement(1131..1346)
                     /locus_tag="I9573_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4798184.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1357..1824)
                     /locus_tag="I9573_003602"
     CDS             complement(1357..1824)
                     /locus_tag="I9573_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAT4798185.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFILENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          745 a          197 c          229 g          653 t
ORIGIN      
        1 tatggaaaaa gatgattaat aaattattaa ttagggtata aaatgatata aatagaataa
       61 ataaggggtg gatgaaatgc ttgtatataa taaaagtttt tatcctaatg acatatttcc
      121 aagattagat ttttcaaaaa taaaaaaaca gttaaaattg atagataatg acctgtcaga
      181 ttttggaaga atatgtataa tagaaaaaga acattatacg ataagtgtaa acagtatagg
      241 tgaaataaat gtgtattatg atttagagta cgaaaataag gtgtatagaa tagtttatga
      301 gattgaaaag ttatttaaat ctcaagttgg aaggtttagt atatctacat acagaaattg
      361 ataattaaaa aagtagaaat taaaaaactt aatactaaag atatagataa atatataagg
      421 taacagctag agagagttgt tacccttttt aaatactaag tatatattat tcttattaat
      481 tttttgtaaa ttcactaaaa gaccttttta tagaatatta ttttcttttt ttattaaata
      541 ggtaaatcag taaaaatgca attccagttc caattattat tccaatgata ttttctataa
      601 ttaatttttt taatataaaa gtaaataata aaaatgctat acataaacct aaaatatatc
      661 ctttaaaata ggttgaaagt tttcttttca tataatcacc aacttgtatc agatttagtt
      721 tacttaatta gagtataaca gtgttgtaac ttcaaagagt atctaaaaga ttacaaatgt
      781 tagataaatc atcaataatg taatttataa cacgtagcta taaactatgc aataatataa
      841 atggaggtat aagtaaatga aaaagttcca tgttttgcta acagtttaaa taaatattag
      901 gtagaaaaga atgtatatag gaatctgtgg caaaattatc aatttaaaag tatgatttag
      961 tgtgatacag ttcttatagg taatggataa caaatagaaa aaggaacagt ttctttctta
     1021 gaacgtattt tgttatgact ttaaacaaag ttaatattaa tgtcaataat atagtaaaag
     1081 aataaaaatc atatagtata ataaaaaaga gtgtttctaa acactctttt ttaatcattt
     1141 tcatcttcta catattcaag tatatcacct ggctgacagt ctaaagcttt gcaaatttcg
     1201 tttagagtgg taaatctaat agcttttgct tttccagttt ttaaaattga taaatttgca
     1261 tttgtaatat ctattttttc tgccaaatct ttaagtgata ttttcctttt tgccatcatt
     1321 acatctaggt taactactat tgccataaca tcacctctat atcgttaaat cattatcttc
     1381 ttttattttt atagctttat caaatgtttc tgcaattaca aagcacatta gtgcaaatat
     1441 aaaagaaata accataagtg gtgagatacc gccactatta tctccaaata tcattataag
     1501 actagtattt gagttataac caattacaca ttcaattata gtgtttatca ataaacaaca
     1561 tcccattact ttaaaccttt ttacattttc aagtatgaat ggggaatact tagtactatt
     1621 aattataaat atcaagaata agatgactat aaagtagtca gaaaaatata gtattcttgc
     1681 aattaaagcg attactttgt cagaactaaa atcatataaa aacataggta tcatacataa
     1741 gaatattata ccagcaactc ctaaaagtga taatagaaaa tatagaaata ctgataatat
     1801 attactaaaa aattttgtaa tcat
//
LOCUS       DACTGW010000362         1774 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.369, whole genome shotgun sequence.
ACCESSION   DACTGW010000362 DACTGW010000000
VERSION     DACTGW010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.369"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            165..611
                     /locus_tag="I9573_003603"
     CDS             165..611
                     /locus_tag="I9573_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAT4798186.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            632..1675
                     /locus_tag="I9573_003604"
     CDS             632..1675
                     /locus_tag="I9573_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAT4798187.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT          732 a          185 c          336 g          521 t
ORIGIN      
        1 tactttgtaa caattttaac aaagtatgtt gatataatac tattaaatta atcttagatt
       61 agtttattct attgatttgt agtttaaatt tatattcaaa aacattgtat aaacatataa
      121 tttatatagg gttttataat tcatatgaaa agagggtaga tagtatgaag ttaatatggt
      181 tgatagtagc agtcttattt ggaatagcag aaatgttaac accaagctta acattgatat
      241 ggtttagtgt aggagctgta atacttatat ttttaagtag ctttatagag agtattttct
      301 tacaaatttt aatatttgca gtaatttcta tagcaatgtt agtcgtagct actaaaaaaa
      361 tagtaaaaaa agataaaggc tataaatcta acactaattt acaagcaatg atgtctaaaa
      421 aaggtattgt tacagaagag atttctccaa ataacacagg tctagttgta gttgaacatg
      481 aaacatggac ggctatttct atagatggtg aaaagataga aaaaggttct acagtagaag
      541 tattaaaaat tgaaggggta aaattagttg ttaaaaaagt agacgccaca gttaatgtga
      601 caaaccaata gaataaagga ggatttatag tatgggtact aaaattgttt taagtattgt
      661 attaatagta gtagtcgtag caataagttt aacgtgtata agagttataa aacaatctaa
      721 agtaggtata ataatgagac ttggtaagtt tcaaaaagtg gctgaaacag gtgtacattt
      781 cttaatacca tttttagata aaatggcata tgtaattgac cttagagaaa ttgtaataga
      841 ttttccacct caaccagtta taactaaaga taatgtaact atgcagattg atacagttgt
      901 atattataaa gttacagatc cagttagata tgtgtttgag atagctaatc caatagctgc
      961 tattgaaaat ttaacagcta caacactaag aaatataatt ggtgaacttg atttagatga
     1021 aacattaaca tcaagagata taataaatgt aaaaatgaga actatccttg atgaagcaac
     1081 agataaatgg ggtataaaag taaatagagt agagttaaaa aatataatgc ctcctcagga
     1141 tattcaagtt gcgatggaaa aacaaatgag agcagaaaga gaaagaagag aggcaatact
     1201 tcaagcagaa ggtaataagt cagctgcaat acttcaagca gaaggtgaaa aacaatctgc
     1261 gatattaaca gcagaagcca aaaaagaagc tatggtacgt gtagcagaag gtgaaaaaga
     1321 atctgctata ttagtggcag aaggtgaagc tgaagctata agacagaccg ctattgctaa
     1381 ggcacaaggt gaggctgaaa tgataaaaag aactcaaatg gcaacagcag aaggtttaaa
     1441 attagttttt tcagcaatga aagaggctga tatagacaat aatattctag cattaaaatc
     1501 tatggaagct cttgaaaaaa tggctgaagg taagtcaaca aaactggttt taccttcaga
     1561 agcagttaat ttcctaggaa cattcaaagg aataaaggaa gttatgagtg atgataacaa
     1621 agaagtactt gatataaaag aagttttaaa tgataatgaa tcattaaaaa aataaaagag
     1681 ttttgttatc ataggaagga tatggaaatt aaaggtcgaa ttaattaaat atagcacaaa
     1741 aatgcacgtt gaaaagaaga ggacttataa attt
//
LOCUS       DACTGW010000363         1738 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.371, whole genome shotgun sequence.
ACCESSION   DACTGW010000363 DACTGW010000000
VERSION     DACTGW010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.371"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     regulatory      171..272
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            345..1688
                     /locus_tag="I9573_003605"
     CDS             345..1688
                     /locus_tag="I9573_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAT4798188.1"
                     /translation="MVPNTNNVQPDGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
BASE COUNT          578 a          252 c          282 g          626 t
ORIGIN      
        1 aatagtataa aacaagttag aataattgac attatactta tacatattca attattgaaa
       61 acagtaattc ttgccaataa ataaccttaa ttttttataa aatcgacaaa aaaacgaata
      121 attttattat atttattgta aataatacaa tattacgata taatacaaca gaaaaacaaa
      181 tagaaaatgt tcatataata ttagtaatac ggtctaatag tttctacaaa ctcaccttaa
      241 atgggttttc tatgaataga atatcaactt tgaatatatg ttctattcta tctatatgaa
      301 tatatatttt tttgtttata tcttaaagtt ttttggaggt aaaaatggta ccaaacacta
      361 acaatgtaca accagatgga aataattcat cattaagctt tttagacaaa atgtttaaat
      421 ttagcgaacg tggctctagc cttaaaacag aagtaatagg tggagttact acattcttaa
      481 caatggcata catagttttc gtaaatccag caatattatc tgaagctgga atggataaag
      541 ccgcccttat aacagttacc atactagcta cagccattgg tacacttata tttgcgattt
      601 taggaaatgc accatttgct ctagctcctg gtatgggatt aaatgcattt ttcacatact
      661 ctttggttat aggtgcagga atcccatggc aaacaggtct tggggtagtt tttctatctg
      721 gatgtgtctt ctttacactc gcagtaacag gtcttcgaaa gacaattgca gacgcaattc
      781 ctagagaatt ggcagtagca tcttcagcag gtataggtct tttccttgct tttgttggtt
      841 taaaaaatat gggtgttata atagctaatc cagatactat agtttcactt gcagatttta
      901 caccaacagt tttaatagct ctttttgctc ttatcttaat ggcaatacta gaatataaaa
      961 aagttaaagg tggaatattg ataagtatag tagttgcaac aataatatct gttatatttg
     1021 gatttgtgga gttgccaaca gcaataatct caacacctcc tagtatagca ccagttgctt
     1081 ttaaattaga tattgtaggt gcattgaaat tctcattaat tggacctata ttctctttca
     1141 tgtttataga catgtttgat tcattagcat ttcttattac ttgttgtaaa caaatgggtc
     1201 ttgaagatga gaatggtgaa ataaaagatt taggaagaat gttatatgca gatgtttctt
     1261 ctactttaat aggctcattc ttaggaacta gtactgttac aacatttgga gaatctgctt
     1321 ctggtatcgc agcaggtgca agaacaggac ttgcttcaat agtaacagca gcattattct
     1381 tagctacact tatatttaca ccattgcttg gtatagttcc tacttacgct gcagctcctg
     1441 cacttgtaat ggttggtgta ttcatgtttg aaagcataaa acagattgat tttgctgata
     1501 gcaaaatagc tgtacctgct tttgttacag ttttattgat gcctttaact tatagtataa
     1561 gtattggttt atgctttgga tttatttcat atataataat gcatgtggta gctaaagaaa
     1621 ctgataaaat cagtattaca ctttggatta taggtttact tgcagttgta aacttagttc
     1681 taagttaata ttttaaatag ttctaggtca atattttaaa tttataaata gcaaaatc
//
LOCUS       DACTGW010000364         1711 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.372, whole genome shotgun sequence.
ACCESSION   DACTGW010000364 DACTGW010000000
VERSION     DACTGW010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.372"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            496..1701
                     /locus_tag="I9573_003606"
     CDS             496..1701
                     /locus_tag="I9573_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAT4798189.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVGPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNVKSIRKSNSAQNIA
                     NLILNIISG"
BASE COUNT          634 a          219 c          226 g          632 t
ORIGIN      
        1 ctcagatact attgttatca aaatatctgg ctttatggtt tgtttcttaa tataaattaa
       61 cctactgttt aattttcaat gttcgtgttt atttcttttt ccttttttca aggacaagta
      121 atactatatc atctttatct attctagtca atataatttt ttactttttt taaatttttt
      181 atttatcatg tattctttat atttaatttt taaaacttaa tgctaagtaa acctatttat
      241 ttcataaaac taatatatat tctatgctct attttccttt ttattctata tttaaatttt
      301 ttatttacaa gaatattttt aatataacat atttgataaa attaatttac atttctttca
      361 cttgtaattt ttgcaatctt gtattagatt aatattaaaa ataatttatc aataacctta
      421 ttcacaaaga cagtttttat atatcaaaat ataatacaat ataaatacta tgctacattt
      481 taggaggatt ttattatgag caaaaaagta ttaataatgt ctgcttccac tggtggtggg
      541 cataataggg ctgctctagc tattaaagaa gaacttactt ccaaaacact tgatggagag
      601 ccaattgaat gtgaaataat agatagtttg aaattagtta ataatacaat ggataaaata
      661 atttcaagag gatacgaaaa atctgctata tatactccca aagcctatgg aagtgtatat
      721 agattatctg aaacaaatct tttgtcaaaa aatgaattta aagataatct actaataaca
      781 ttcatggcta aaaaatttaa aaagcttatt agaagcgaaa aaccagactt gattatagga
      841 acacatcctt ttccaatgat tgctctaagc acattgaaaa aaaactttaa tttacataat
      901 aatgaatcaa atgcttacac agagcatttt tataaacatt atacaaatac cattaatgtt
      961 cctccgctta tttctgtatt aaccgattat actactcatt ctacatggat acaaaatgaa
     1021 attgattact atattgttgg tcatgaatat gtaaaagaat tattggtgtt tgatggagtt
     1081 ggaccgagta aaattagaac atttggaatc cctgttgaaa aatcttttct ctctcacaga
     1141 gataaagata ttgttctttc tgagttgaat ttatctcctg ataaattaac tgttttactt
     1201 atggggggta gctttggggc tggaaatata aaagagacac ttgatgaatt actggatact
     1261 gatagagatt ttcaaatact tgtaattact ggtaagaatg aaagcctaaa agaaaaaatc
     1321 gaaaaaaaac ttatgtctcg ttatcatgat aaaaatgtat gtgttcttgg atacactgat
     1381 aagatgaatg atattttagc ttctatagat gtattagtaa gcaaacctgg tggacttact
     1441 acaactgagg ctcttttaaa agatgtacct atgatagttc cttattatat accaggtcag
     1501 gaagaagaga atcttgattt cttatctaat tgtggtgcag cacttagaac tacaaaaaaa
     1561 tataacttgt caatattact aaaagttcta atagatgacc ctagcagatt agaaatgcta
     1621 aaaagaaatg ttaaatcaat aagaaagtca aattcagccc aaaatatagc taatttaatt
     1681 ttaaatataa tttctggttg ataaaataaa t
//
LOCUS       DACTGW010000365         1685 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.373, whole genome shotgun sequence.
ACCESSION   DACTGW010000365 DACTGW010000000
VERSION     DACTGW010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.373"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(131..1522)
                     /locus_tag="I9573_003607"
     CDS             complement(131..1522)
                     /locus_tag="I9573_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAT4798190.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
BASE COUNT          602 a          310 c          187 g          586 t
ORIGIN      
        1 ttttaattat aatatctatt tgtgtaaagc tttttttcat aatcaaagag gaaattacat
       61 ttaattttat atttaaatgt aacttccttt atttgaagta attcatctat ttacaataat
      121 tcgtcatgaa ttatttttct tttgttagta aatccatagc cattaatctt gttaatacat
      181 ttcttattcc caataatatt aaaccaaata ttattaaaac tgttgctatt cctccaaata
      241 tctgtaaaaa accaagtgtg gctgtaaaac cagcaatctt tcctgataat aaacttgcaa
      301 aaaaacttgt tagataccag gctcccatag ataatgtagc atacttagct ggagctaatt
      361 tattgaacat tgcaagtcct ataggagata agaataattc acctactgtt gtaaatatat
      421 aagtcaatac tataaacaat atactagcct ttaccattcc atctggacca ttagctaatg
      481 tagcaaatac cataactata aacccaacac ccattattat cattcctaat cccattttaa
      541 ctggaataga gaaatcacct cttttggtat gagataattt aacccatagg gtagctaata
      601 ctgggcataa agccacacat aatatagcat ttagtgatgt aagccatgct actggcattt
      661 caaatccaaa tagagttcta tttacattat cccttacata aatcataaaa gaactttgtg
      721 tttgatagta tgctgtccag aataagacca caaataaaaa taaaataacc attgctatta
      781 tcctattttt ctccaactta gtaagtggtc catactcaat tttttcacct ttattacctt
      841 tagtcacagg atattttccg acttcattta ataattttgg tgcaagaagt gtaaatataa
      901 tcatacctaa catcatgcct ataccacaaa ttaaaaatgc catcttatat ccataagata
      961 ctatatctcc acttgctcct cttactgcaa atacattatc agaaagcaat ccacatatta
     1021 ttggagctat aaatgctcct acatttactg ccatatagaa taaactgtat gctgcatctt
     1081 ttcttgacac atcagtttta ggatatatat ccccaactat atttgaacaa cttgatttaa
     1141 aaaaaccatt tcctaaaata attattgata gacctaccca aaccatggca atagatgtag
     1201 atgaagcaaa tagtacgaaa tctcctattg ctattaaaag tgcacctata gaaatacatt
     1261 tttgttgacc taaaacttta tctgctaacc agccaccgac taatggtgta agatatataa
     1321 gacctgtaaa taagccaaac atggatgttg cttcttgagt agaaagacct aaaccacctt
     1381 gagctacact tgctgtaaaa aataatacca ataaagctgt taaaccataa taactaaatc
     1441 tttcccacaa aattgtcatg caaataagcc aaaaactctt cggatgacca ctttttttag
     1501 atacgctttc tgtacttgac ataaaaaaac gccccctttt gttttatagt tgaatttaat
     1561 attaatattg ctttaaccat tttagcacat acttataata tcatcttttc tatgtttact
     1621 caatagatta tcgcctgtat tttcattttt ttattattat ttttaaaatt atgaaaaata
     1681 ttatt
//
LOCUS       DACTGW010000366         1667 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.374, whole genome shotgun sequence.
ACCESSION   DACTGW010000366 DACTGW010000000
VERSION     DACTGW010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.374"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(93..926)
                     /locus_tag="I9573_003608"
     CDS             complement(93..926)
                     /locus_tag="I9573_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAT4798191.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVFIVLLIIGVYKLFKKY
                     N"
     gene            complement(1255..>1667)
                     /locus_tag="I9573_003609"
     CDS             complement(1255..>1667)
                     /locus_tag="I9573_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAT4798192.1"
                     /translation="GIDYENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMG
                     ASKKGSYGASIIEKIESIIPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQ
                     QLNNHEESQSHPDEQEIYEASMFAKNMMIKASIV"
BASE COUNT          562 a          274 c          165 g          666 t
ORIGIN      
        1 aatttcatat acaaataata aaatagccac tacaatccat aatttgcagt ggctactttt
       61 tacactatca cttattactt aatttttaaa aattaattgt attttttaaa taatttatac
      121 acacctatta tcagtaaaac aataaataca agtatatcta taaaaataat tattgtaaca
      181 tgtctggcaa tattgtaaat aaaaagtata gagaatattt ctatagacaa cgctacaaaa
      241 cacataaaat tcgttaaata atctgcaata gtatcactat aaaaagcatg tatttgtaac
      301 catctccata taaatggacg taaaaggatt ctgaatacaa cacaaatgac agggtaggca
      361 taaataaatt ttttatctac ataattagct acaccattat tccattggat aggaatttga
      421 ggtggcaact ttggaaatac tgttattgca atcaacaaac aagcaatagt aataattccc
      481 caggtaattt tccctcccca tatatataag aaacttacag agtcaagttt aaaaacaggt
      541 ttgtttttat tccagtattc tggcaactca gttatgtatt gctctacttt caaattcaaa
      601 taacttagct catcagattc taacatttta ttacacatag tttttgcatt ttctaagtct
      661 gatattttag tttcgagaac tttctcctgt ttctctacaa cttcaactaa agatactttc
      721 tgtaatataa tattacggat atcatcaata gtaattccta gtgtacgtaa atatgcaatt
      781 tttttgatgt cctctacatt ttcatctgaa tattctctat agccattacg ctcattacga
      841 aggggtttaa ttaaattctc gttctcataa aaacgaatat tagagcgtgt caatcctgtt
      901 ttttcttcaa cttctttaat tgtcatttta aacactacct ctctattcta ttaatttaca
      961 actataatat agtcttaaat attttcactc tataaaattg tatatttagt atatcttaaa
     1021 atcaagttaa ttatctttta taaattttca tacaataata tctcctttca ataatatctc
     1081 tttttctata tttaaaacta tcttattttc tataaaataa tgtataaaca aggtttacag
     1141 tcaagtattt ttattatttt tttgatttca taaaaaaaag catagcaaat catttgctat
     1201 acttttttac taaattattt attctatatc ttatatgaac ttattgttta actactacac
     1261 aatactagct ttaatcatca tattttttgc aaacatactg gcttcatata tttcttgttc
     1321 atctggatga ctttgagatt cctcatggtt atttaattgt tggcgaatat gttcgttgtc
     1381 aggagaaagt tttaacatct ctttatattt tgctttcaat ccttctgcta tcttaccttg
     1441 acatatgaat gaacctaata tttcattatc ttttgggata attgattcta tcttttctat
     1501 tatactagct ccataacttc cttttttact tgctcccatt gttccaaata gtgctatctt
     1561 tttattgtga atattttcaa gcacttgttt tgaattttta tcacaaattc ccttgtccac
     1621 ccaatagcct aagaatacta tatcataatt ttcatagtca ataccat
//
LOCUS       DACTGW010000367         1662 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.375, whole genome shotgun sequence.
ACCESSION   DACTGW010000367 DACTGW010000000
VERSION     DACTGW010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.375"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>1662
                     /locus_tag="I9573_003610"
     rRNA            <1..>1662
                     /locus_tag="I9573_003610"
                     /product="23S ribosomal RNA"
BASE COUNT          477 a          347 c          492 g          346 t
ORIGIN      
        1 gggacacgtg aaaccctgtg ggaagcagga gggaccaccc tccaaggcta aatactacct
       61 agtgaccgat agcgtatagt accgtgaggg aaaggtgaaa agaaccccgg gaggggagtg
      121 aaatagaacc tgaaaccctg cacttacaag ctgtggaagc acatttcttg tgtgaccgcg
      181 tactttttgt agaacgggcc aacgagttac gttaagtagc aaggttaagc acttaaggtg
      241 cggagccgta gcgaaagcga gttttaactg agcgttcagt tacttgacgt agacccgaaa
      301 ccgggcgacc tacccatgag caggatgaag cgaaagtaaa atttcgtgga ggtccgaacc
      361 cacgagcgtt gaaaagctcg gggatgactt gtgggtagcg gtgaaattcc aatcgagccc
      421 ggagatagct ggttctcccc gaaatagctt tagggctagc ctcaaggtga gagatacgga
      481 ggtagagcac tgaatgtcct agggggtatt gcacctaccg aagactatca aactccgaat
      541 gccgtcatct tatacttggg agtcagactg tgggtgataa gattcatagt cgaaagggca
      601 acagcccaga tcgtcagcta aggtccctaa atgtaagtta agtggtaaag gatgtgggat
      661 tgcacagaca accaggatgt tggcttagaa gcagccactc attcaaagag tgcgtaatag
      721 ctcactggtc gagtgatcct gcgccgaaga tttccggggc taaaacttac taccgaagct
      781 acggcatcag taatgatggg taggggagct tcccatacgg gttgaagcat gaccgtaagg
      841 acatgtggac agtatgggag tgagaatgtt ggcatgagta gcgagatgtg ggtgagaatc
      901 ccacaggccg taaacccaag gtttccaggg gaaggttcgt ccgccctggg ttagtcggga
      961 cctaagctga ggccgaaagg cgtaggtgat ggacaacagg ttgatattcc tgtactaccg
     1021 ataaccgttt gagagaaggg atgacacagt aggataagct aagcacactg ttggttatgt
     1081 gtgcccaagc attgaggcag tcaaagtagg caaatccgct ttgataatgc tgggatgtga
     1141 tggggagcga aatttagtag cgaagtagct gatttcacac tgtcaagaaa agtctctatc
     1201 gaggttaaag gtacccgtac cgcaaaccga cacaggtggg tgaggagagt atcctaaggc
     1261 cagcgagaga actgttgtta aggaactcgg caaaatgacc ccgtaactta gggataaggg
     1321 gtgccaccat caggtggccg cagagaatag gcccaagcga ctgtttacca aaaacatagg
     1381 tttctgctaa gtcgcaagac gatgtatagg agctgacgcc tgcccggtgc tggaaggtta
     1441 aggggatctg ttagagcaat cgaagcagtg aacttaagcc ccagtaaacg gcggccgtaa
     1501 ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgaccc gcacgaaagg
     1561 cgtaacgatt tgggcactgt ctcaacaaca gactcggtga aattgtaatt ccggtgaaga
     1621 tgccggatac ctgcgacagg acggaaagac cccatggagc tt
//
LOCUS       DACTGW010000368         1613 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.376, whole genome shotgun sequence.
ACCESSION   DACTGW010000368 DACTGW010000000
VERSION     DACTGW010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.376"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(193..1530)
                     /locus_tag="I9573_003611"
     CDS             complement(193..1530)
                     /locus_tag="I9573_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAT4798193.1"
                     /translation="MDTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
BASE COUNT          617 a          197 c          203 g          596 t
ORIGIN      
        1 aaaaagagtg cctcatcaac taaaaagttt attttgagac acacacattt ttattaaggt
       61 aaataaaaat atcacatttg attaaatttt aagttaatga atatatgtgt aaatatttaa
      121 ttataagtta ttataatttt acatagtata aaacaatcta atttgtatta tataagataa
      181 tctaatttta gactaaaata atctaatata aagaaataga gaaaattcat tctcagtata
      241 agagatatcc aattttccat tgtacttatt aacaatttct ctaatattct caataccata
      301 tccgtgagaa ttactatctt ttttatctga aataaatagt tcttgttttc tattgatagg
      361 atttaccttg ttgtttgtca ataaatagct aaaatactcc ttctcacaac gtgcctttaa
      421 taccactttt ctatttgaca aatcaggaat tttcaaagat gcttctatag cgttatctaa
      481 agaatttgaa aaaatagtac ataaatctat aggttctata cccaagtgat tgtctatatc
      541 tatattaaag tgcacatcaa caccattttc ttccaaactt gtatgacgaa gatttaaaat
      601 tgagttaaga gcttggtttt tgcaaaattg tttgccacta agagtaccta tacctatttt
      661 taactgatta aaatactttt tagctccagc aatatcttca atttctaaat acgattcaat
      721 catttgaaag tgattattca tatcatgtcg cattttgcgt atttgaattt gttgattttc
      781 tagtaagtta tagtattttt cttttaggtg atttgcttgt ttttctatct tcatctttac
      841 attttcaatc aatggctgta aaagaagtat cacaccgata tttgccagaa ttgctgacaa
      901 gatgattaga taagtgccat aaggtaagtt gcctaagata atattcttat tactacccat
      961 agggattaaa ttagtaggtg gcagaataat agcaattaat attgataaaa atgaagatat
     1021 acataccata tcaataaaaa tccatatttt atttgataag tattgtttta tattgaccaa
     1081 tctattcttt aggaaaatat gtattaaaaa ccaaaataat aatttaatta gagcagaaat
     1141 tatctggact attgaaagta ttaaaatata ttcttttgag tgtataagtt cctcatatat
     1201 agggttgttt ataaaaagaa aattaagccc aattataata ggataaagta tcatgacaac
     1261 tgatattata ggaattaatt tgcttttgct acatattata agtagtataa aatatccgaa
     1321 tagtgtaaac aaaatattta cggggtcgga aagccatatt atcatttgac taataataga
     1381 gtagcccaaa atcaatatga ttttgaaaat ccaatgattc ctaaagtcta taaaatagga
     1441 agtcaactta taaagaaaaa aggctatgaa aatattgatt aatattagaa taaaatttat
     1501 gcttgtagag attaaagaat aagtgtccat ttaatatacc tttctattaa ttaagattct
     1561 ggaaactaag ttttaaaaaa ttaaatctta aaaattaaaa ttctggaaat tag
//
LOCUS       DACTGW010000369         1601 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.377, whole genome shotgun sequence.
ACCESSION   DACTGW010000369 DACTGW010000000
VERSION     DACTGW010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.377"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..310
                     /locus_tag="I9573_003612"
     CDS             <1..310
                     /locus_tag="I9573_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAT4798194.1"
                     /translation="NKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDS
                     RAFQLRVTDKGEEVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEG
                     "
     gene            312..1406
                     /locus_tag="I9573_003613"
     CDS             312..1406
                     /locus_tag="I9573_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HAT4798195.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1494..>1601
                     /locus_tag="I9573_003614"
     CDS             1494..>1601
                     /locus_tag="I9573_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAT4798196.1"
                     /translation="MKSLVLYSSLTGNTKKIAYAIYDEIQEEKDIKDVNE"
BASE COUNT          651 a          161 c          302 g          487 t
ORIGIN      
        1 taataaaaat aggaattgtt ctttgaatga tgtttctaca gaatttgaag ttgataaagc
       61 tcatacaaca agaactatat ctaggttaga acaagatgga tacatagaaa aaatccaaaa
      121 ccctaatgat agtagagctt ttcagttgag agtaacagat aagggtgagg aagtattagg
      181 ggatataaaa aacatatttt caaagtggga taaccacata aaaaaagagt tctcagatac
      241 agaatataaa gaactggtaa agaatttaca tgtggtaaag gatataaaaa cagctgtgga
      301 ggaaggatag gatgtattgt aatttattaa aaccaattaa aattggaaat ttggaattaa
      361 aaaatagagt atcttttgca cctacaagca tgggtcttaa attggaagaa aaaattaaaa
      421 agttttctga tattgctaag tcaggagtag ctctgattac tctaggagat gtatctatta
      481 gacctagttt tcataaggta gctataagcc tatcggatga agatggagtc attaaataca
      541 agaaaattgt agatgaaata cacaattctg gtgcaaaggt atcagcacaa ctattttgtt
      601 cagattatga tgttaattta attaaagata ctatgaagat gggaattact tcacatgatg
      661 agataaaaaa aataatgaat gacggagtaa aagattacat aacaaatatg cccaaagaag
      721 aaattaaaaa tattataaat ttatttaaag taacagcatt gaatgcaaaa aaagcaggtt
      781 ttgacatgat tcaaattcat ggtgataggc ttgtaggaag tttttcatca agtatattta
      841 ataatagaaa tgatgaatat ggtggaacgt gtgataatag aagtagattt gcaagcgaaa
      901 taattagtag tatccgagat gaagttaagg acattccaat agactataaa tttgcaataa
      961 gacaagaaaa tcctcattat ggaaatgcag gagttttact ttcggaagtt gagtattttg
     1021 ttaaaaaatt tgaatcccta ggtgtaaaca gttttcatgt aacacttgca aatcattcaa
     1081 aattagaaga tactatacca accaataatc atccttactt taaagatgaa ggctgttttt
     1141 tatatttagc tgatgaagta aagaaacaca caaatcttcc agtatgtggg gttggaaaat
     1201 tgtctagccc agattttata gaaagtatta tttcaaataa tagagtagat atggtttcta
     1261 tgtcaagaca attactagca gatagtaatt ggttacagaa agtaaaagat ggaagagtag
     1321 atgagattaa aaagtgctgt tattgtaata agaagtgtgc agatgcttta caaacacgct
     1381 ctcagtttgg atgtatacta gattagaata tctttataaa aataaataat ttgttaaggt
     1441 taatatattt ttggtgattt agataagtat ttagataaag aggaggagac atcatgaaaa
     1501 gtttagtttt atattcaagt ttaactggaa atactaaaaa gattgcttat gctatatatg
     1561 atgaaattca agaagaaaaa gacattaaag atgtaaatga a
//
LOCUS       DACTGW010000370         1540 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.378, whole genome shotgun sequence.
ACCESSION   DACTGW010000370 DACTGW010000000
VERSION     DACTGW010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.378"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            202..1008
                     /locus_tag="I9573_003615"
     CDS             202..1008
                     /locus_tag="I9573_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAT4798197.1"
                     /translation="MGMILSTLGINLYLAPAKLLSGGVAGICVILYKLFGINQGISSF
                     LMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLTQDIAKYFPIDDTMLQCIYGGAL
                     TGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNTFLFINFFVVCAGAFLFGAKLVM
                     YTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEISKVIMNKMGRGVTFLEAEGAYT
                     QNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNNVEEVKGGGFKDKFL"
     gene            1135..1407
                     /locus_tag="I9573_003616"
     CDS             1135..1407
                     /locus_tag="I9573_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798198.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
BASE COUNT          575 a          160 c          294 g          511 t
ORIGIN      
        1 ataaatatat taaaagaagg attaagacta ataacaattc aaaataaata acttatgtaa
       61 gaaccgtagg aactacgggg atagcttgtg gagaattagt aagacatatt ttagtatgca
      121 aaattctatg aagcaggaac cctgtgactt tagtcatggg aggttcagaa taacatattt
      181 ttacaatatg gtctgattat attgggtatg attctatcaa ccttaggaat aaatttatat
      241 ctagcaccag ccaagttatt aagtggagga gtagctggaa tttgtgttat attatacaaa
      301 ttatttggta taaatcaggg aatatcaagt ttcttgatga acataccaat atttattata
      361 gctagaaagt attttgataa taaatttttg ctaataagtt ttgtcaatat gttactgttt
      421 tctctagcac tggggctaac acaagatatt gctaaatatt tcccaataga tgatactatg
      481 ctacagtgta tttatggtgg tgcattaact ggtataggta tggggttaac ttttaaagca
      541 agagctactg ctggaggact tgatataata gcagccataa tgaaaagaaa gtatgatata
      601 cctatgaaaa atacattttt atttattaat ttttttgtag tatgtgcagg agctttctta
      661 tttggagcta aattggtgat gtacacttta attacaatgt atataatttc ttttactatg
      721 gatataggaa aagattgctt tgatagaaaa aaatctatac ttttaatatc aaataaatat
      781 gatgaaatat ccaaagtgat aatgaataaa atgggtagag gagtcacttt tttggaggca
      841 gaaggtgcat atacacaaaa taagaaaaag atgatttatt gcataatatc tgcaaatgaa
      901 ataggtaaat ttaaagattt aatttatgat atggatagtg aagcttttat ttctgtcaat
      961 aacgttgaag aagtaaaagg tggaggtttt aaggataaat ttctttaaga ttatatatgt
     1021 taattatata tgttaaaaaa tgccatatgt attgagatta tacctagtta gatgtatact
     1081 gtagttaatg gggtacatga agtactcaat aatatactag gaggtataag aattatgtta
     1141 agagagagat ttgtagtaag aaatcatcat aatccacata caagtgggtg ttgtgggact
     1201 ggaggagaag gatgctgtgg aggagataac catgaacatt cacatcaaca ttctcacagt
     1261 catgggcatg aacatgagca tgtaaatggt gtaggtcacg accataatca tgatgatggt
     1321 ttttcaggta ctcatgagca tagccatgag catacacata cacatggtca tgaacattca
     1381 gatgggcata cacatgaaca taaataaaaa cattttaaat ttataagaga tttttatttt
     1441 gtgattccat gaaggtttaa ataaaaattg aatgttttaa gtaaaaatta ttgagaataa
     1501 ataagaaagt gaactgttaa agtagataac ttaaatagtt
//
LOCUS       DACTGW010000371         1537 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.379, whole genome shotgun sequence.
ACCESSION   DACTGW010000371 DACTGW010000000
VERSION     DACTGW010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.379"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(12..1304)
                     /gene="brnQ"
                     /locus_tag="I9573_003617"
     CDS             complement(12..1304)
                     /gene="brnQ"
                     /locus_tag="I9573_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAT4798199.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVDVPVV
                     KSLPLHSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT          558 a          280 c          190 g          509 t
ORIGIN      
        1 ttaaacaaaa cttagtttgt tccaagtgta ttagattgac tctttgatgg tataaaatta
       61 ccaattattc cagctattat aactggtacc acccagttaa atcctaatga atgaagaggt
      121 aaagatttta ctactggaac atctactcca taactagata taactgttag tatacttaca
      181 agcaatgaca tatatactgc tcctttgtac acattatcat tcttaatttt ttcattgaag
      241 aaagctaaaa ttattaaaac aattgatggt ggataaacta tacttagtat aggagatgct
      301 atctttatta tagttccaac accaaaagat gctactactg cactgaatac agatacagct
      361 aatactattt tttcataact caatttacct tttgtaagtc tagagaaata ttgacctgtg
      421 gcagatgtaa gacctataga tgtagttaaa catgctaatg atactactac tgctaaaatc
      481 gctttcccaa catttccaag taatccttca gttatattta ctattacttg tgattgtact
      541 gcatctgttc catacattgt tgatacagtt gctccaagga aacataatcc tccataaact
      601 agagctaatc ctccacaagc aagaaccccc gccttaccaa tcatttttaa cttgtcacta
      661 atagtagaat atcctttatc atttaaagat actattaaca ctgatgcaag tgctattgct
      721 gcaaaagcat ccattgtctg atatccttga cctatacctt ctgcaaatac attaggtatc
      781 ataggtttat ctactatatc tccaagaggt gatataatac cttttataat tataaatgct
      841 agagctatta aaagagctgg agttaaaaac tgtccaacta tatctacgac cttagaaggt
      901 cttattgtta gtacatatga aagcgcaaaa aaaagtatac agaataataa cactggaatt
      961 gaagttccaa ataaaggcat tatacccatt tcataagttg ttgcacctgt tcttggtata
     1021 gctaagaatg gacctataca taaaatgtct gcacaaccta taagaatccc taacttcata
     1081 ccacatcttt taaacatttc aactacgttt ccatcgaatt tagctgtagc cattactgct
     1141 agaagtgcca atccagcatc agcaaacgta aatcctaaga aggcaattaa ccattctgga
     1201 cctgtaatta ttcccaagta aggtggaaat attaagttac ctgctccaaa gaacattgca
     1261 aataacgcaa atccaaatac tataacatcc ttggtcttac ccattataaa atcccccttt
     1321 ttaaccttat taaattaata aaatgtctcg attgtttacg cttgcataaa taaattaaat
     1381 taaacaataa gaacttatta ttaaattaat aaaattaaat aaattaaatt tttaatatat
     1441 tgtatttatt tgtgcttatt atatattata cgaaacaagc aagtcaatat gctaaaattt
     1501 aaaataatca cactcatttt aattttcaaa atattag
//
LOCUS       DACTGW010000372         1504 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.380, whole genome shotgun sequence.
ACCESSION   DACTGW010000372 DACTGW010000000
VERSION     DACTGW010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.380"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            60..1460
                     /locus_tag="I9573_003618"
     CDS             60..1460
                     /locus_tag="I9573_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAT4798200.1"
                     /translation="MNQEIFFKIMNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMN
                     RHDILANSFFDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPID
                     IKGEFVGALEISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAI
                     EKSKKACKSDASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGIL
                     FGTSKGGFTGAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTK
                     DIPIDVRIIATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDILLLCKKFI
                     SKYNKKLNKKVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINL
                     NDYYNLKENKESNNYSLNDLEGKTLSKIISEIEEKYIYESLEKASYNITKAASILGMN
                     RQNLQYKIKKYNIKIL"
BASE COUNT          656 a          151 c          272 g          425 t
ORIGIN      
        1 atagcaatat tttattaatt ttttgggttt ttatatattt aggtactggg gtgatatagt
       61 tgaaccaaga gatatttttt aaaataatga atgaaatatt ggaaaatatt gatgaagggg
      121 tacattttgt taataaagac gggaaaacta taatctacaa taattctgta caaaaaatgg
      181 aaaaaatgaa cagacacgat attttagcca attcattttt tgatatagtc aacaaaatga
      241 aaataaacag gagtacacta ttagaagttt tggaaaaaag ggaagttatc aaagacaata
      301 ttcaaaaata tctggacaaa aatggaaaag agataacagc aattaatact acaataccaa
      361 ttgatattaa aggcgagttt gtaggggcac ttgaaatatc aaagaatatg acaacgatag
      421 aaaatttact aaacagaaat tataaaaaaa atttagtcaa taaagttagt agtagacaaa
      481 aaaagggtta tgaatttgat gatattttag gggaaagtaa tttgattaaa gaagcaatag
      541 aaaaatctaa gaaagcttgc aagtctgatg cttcagtatt tatatacggt gaaactggaa
      601 cgggtaaaga gttatttagc cagtccattc attatggaag taataggaga aactatccat
      661 ttatagcaca aaattgtgcg gctttgccgg aatcactatt cgaaggtatc ctttttggaa
      721 ctagcaaggg aggatttaca ggagcaatag ataggccagg cttatttgaa caagctaaca
      781 aagggacatt gttattggat gagataaatt ccatgcctat tatgttacag gctaaactat
      841 taagggtttt acaagaaggt tatgtaagaa gggttggagg aactaaggat atacctatag
      901 atgtaagaat tattgcaacg acaaatgaaa gtccaaaggt aattttaaac gagcataaaa
      961 tgcgaaaaga tttatattat aggcttaaca taattcatat aaaaattcct cctcttagag
     1021 atagaaaaga ggacatactt ctattgtgca aaaaatttat ttcaaagtat aataaaaagt
     1081 taaataaaaa agtggtagga atttcagatg aagctattaa aattttaaaa aattacgatt
     1141 ggcaaggtaa cataagagag ctagaaaaca taatttattg tactatgagt atgatggaag
     1201 atgaggtaca aatagaaaca agtatgatta atctaaacga ctattacaat cttaaagaga
     1261 ataaagagag taacaattat tctttaaacg atttggaggg caaaacactt agtaagatta
     1321 tcagtgagat agaagaaaag tatatatatg aatcattaga aaaggcatcg tacaatataa
     1381 ctaaagctgc atcaatttta ggtatgaata ggcaaaattt acagtataaa ataaaaaaat
     1441 ataatatcaa aatattataa ataagcaaga taaaaaaaca aaatagaagt aaaataaaaa
     1501 agca
//
LOCUS       DACTGW010000373         1504 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.381, whole genome shotgun sequence.
ACCESSION   DACTGW010000373 DACTGW010000000
VERSION     DACTGW010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.381"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..799)
                     /locus_tag="I9573_003619"
     CDS             complement(<1..799)
                     /locus_tag="I9573_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAT4798201.1"
                     /translation="MLTNLSIGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIFIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMI
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICI"
     gene            complement(793..1503)
                     /locus_tag="I9573_003620"
     CDS             complement(793..1503)
                     /locus_tag="I9573_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4798202.1"
                     /translation="MISIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSSGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
BASE COUNT          537 a          213 c          118 g          636 t
ORIGIN      
        1 ttatacaaat catatggttt ttcaaatcat gataaacata ttttaatcta tcatgaactt
       61 cgtttatatt ttcataattc ctgtagtttg tttttattct ttcatttata agttcatact
      121 ctagatttaa tttatcgtct tgtacaattt tccctattac tattaaaagt attatacttg
      181 atgataaaat caataaaatt gcaaatataa atataaataa gttttctgta gtaattgtat
      241 tgactcttaa attatattca aatataagta ataagctaac tatattagat aatataggta
      301 ttcctattag tatcatatct ttaggtttaa aatctaaaga taatttaaaa tacttaaaca
      361 atataaaacc tataaataaa aatacctttg acataataat agcttgtatt ctaaataagt
      421 tttcatgttg tgttatgtta atattattta atttatttat aaatactact agacctactg
      481 cagaaccttc agcaatcatt aatcctagcc aaaacaataa actaactatt atacttttat
      541 atattttatc ttcatatgat gttatataaa atgatattcc tattaccata catagaagaa
      601 tcttaatatt tggaaacact ttaactacat ttatgtatgc cataaatata aatataatta
      661 ttgttgtaaa tatatatctt atattagata ttttcctctt tccaactaag tggattacta
      721 aaaaaaatat aatccaatct atagtacctg aaactaaatt aataatattc caaaatccta
      781 tacttagatt tgttagcata ctatatcccc taatatatta gttatagcaa gtttcaagcc
      841 tttaactcta tatttactta ttggaatact tttgccatct actataacag tacttccact
      901 cattgattct actaatttta aattaactat atagctatta tgacatctaa aaaatccata
      961 ttcatttaat atcttttcta ttttagatat actcatttta gtaatataca tatcatcatg
     1021 tgtataaatc aatatattcg gtctatctgt ttctatataa gtaatagaat ctattttaat
     1081 tcggtctaca taatttttta catttatagt taagtaatta tttctctttt tcatcatctc
     1141 atttatacaa ggtaatacac ttttagaaat tttttcttta tttattggct ttagtatata
     1201 tctgtatgct tttacctcat aaccttcttg cataaactct acaaatgaag taacaaatat
     1261 tatttctaac ttatgatcaa actctcttat cttcctagca gtatccatac catttattgt
     1321 tttcatttgt atgtccataa ttaatatgtc taaatcttta ggatagttat ttagtaactc
     1381 ttctcctgat gaaaattcat atatgttata atttatagga tatgaactta atatttcact
     1441 taatatatct tttattttta ttcgataatg tagttcatca tcacatattc ctatactaat
     1501 caca
//
LOCUS       DACTGW010000374         1503 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.382, whole genome shotgun sequence.
ACCESSION   DACTGW010000374 DACTGW010000000
VERSION     DACTGW010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.382"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     repeat_region   267..1482
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=267..295
                     /rpt_unit_seq="gttttatattaactatgtggtatgtaaat"
BASE COUNT          489 a          149 c          244 g          621 t
ORIGIN      
        1 ataatatagt aagtatttac aaatatgtag gtgttcttaa attgataaat tattccattt
       61 taattttata gtttgaattt tatgatataa taaaaatata taaattttgc agtgagcgat
      121 atttttgata aagtagggtt taacagttgc aatgtaaggg attgagggtg tatgataaat
      181 gttatcaatt gcactactca tggttcactg caaatttgag agagttgtat gtgtgtaagt
      241 actgaaaata cttagtttat tttggggttt tatattaact atgtggtatg taaatttaat
      301 ttatctataa tttaaaacgg tgaattacat atgttttata ttaactatgt ggtatgtaaa
      361 ttaaagaggg gtacttcgta cttttaccac tcaaactagt tttatattaa ctatgtggta
      421 tgtaaatagg tcaagcatag attttgcaac tggaagataa gaagagtttt atattaacta
      481 tgtggtatgt aaatgtttta gaacagaaaa aattgtataa attgtggaat ggttttatat
      541 taactatgtg gtatgtaaat ttgtaaaatg taaatagcat attaattttt ctttcacgtt
      601 ttatattaac tatgtggtat gtaaattgga gcattatgtt tttaggttac ggagcaacat
      661 ttagttttat attaactatg tggtatgtaa atggtgatag tggaagtttt acttatactt
      721 ggcgtgctgg ttttatatta actatgtggt atgtaaattt cagaatactt agctcctgcg
      781 cctatatcgt ctgaatgttt tatattaact atgtggtatg taaataaagt tttgcgcatt
      841 gtatgtgtgc ctagattatc tgttttatat taactatgtg gtatgtaaat atgtccccac
      901 atcaattttc ctccgcatga aaaaatcgtt ttatattaac tatgtggtat gtaaatttta
      961 gaaacaacag aataaacata atatataagc atagttttat attaactatg tggtatgtaa
     1021 atctatatcg catattcttt tatctaggtc ttcttttcgt tttatattaa ctatgtggta
     1081 tgtaaatata atcttgttat agactttact attgttgtaa tagagtttta tattaactat
     1141 gtggtatgta aatcttacca gaccctgcaa cactaaatga cttacatagt tttatattaa
     1201 ctatgtggta tgtaaattca tcagtatttg taaaatgctt taacttatca taatgtttta
     1261 tattaactat gtggtatgta aatttttact tttcctttct atgtgtccaa ttcggacaca
     1321 ttgttttata ttaactatga ggtatgtaaa tcttatactt agttagaact atatatcgac
     1381 acaaatatgc tttatattaa ctacatgata tataaattgc aatttttatt cgttgtccaa
     1441 tcgcttgaaa tttgttttat attaactacg tggtatgtaa atttgcaaca agtataggta
     1501 aaa
//
LOCUS       DACTGW010000375         1480 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.383, whole genome shotgun sequence.
ACCESSION   DACTGW010000375 DACTGW010000000
VERSION     DACTGW010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.383"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1344
                     /locus_tag="I9573_003621"
     CDS             <1..1344
                     /locus_tag="I9573_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAT4798203.1"
                     /translation="GILFTSKDGEINTYNSKVKDILDINEDIYGKYIDDIFVDSLKVL
                     LSEKEILDKVVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREK
                     GQIAKYTFKDIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNR
                     KNQPFIAVNCAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDM
                     PLELQTKLLRVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLN
                     TIPINIPPLRERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLN
                     IMCEDIVLEKDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLIL
                     KALLDKNLQITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT          666 a          152 c          222 g          440 t
ORIGIN      
        1 ggtatattat ttacttcaaa agatggtgaa ataaatacat ataatagtaa agtaaaagat
       61 attttagaca taaatgagga tatatatgga aaatacattg atgatatatt tgtagatagt
      121 ttaaaagttt tattgagtga gaaagagata ctagataaag ttgtcgtatt caataagaaa
      181 tatataaatg taaataaaaa aaacatatac aatagggatg aaaaaatggg cacatattat
      241 agtttacaag aaataacata cataaaaaaa ttagaacaaa atctcactaa aaaattaaga
      301 gaaaaaggac aaatagcaaa atatacattt aaggatataa aaacaaatag tcctaaaatg
      361 tttgaatgta tagatttagc taagaaggtt tctaaatctg acttaagcat acttatcaga
      421 ggtgaaagtg gaactggtaa agaactcata gctcagtcta tacataacaa ctcaaataga
      481 aaaaatcaac cttttatagc agtaaattgt gcagccgttc ctgaaaattt attagaaagc
      541 cagctattcg gatatgacaa gggaactttt actggaggtc taaaagatgg aaaacaagga
      601 ttatttgaac ttgctaataa tggaacaata ttcttagatg aaataggcga tatgccttta
      661 gaattacaaa caaaactact tagagttctt caagagaagc agataatgcc tgttgggtct
      721 cacaatgtaa ttaatataga tgttagaatc atatctgcta caaataagaa tttagaacaa
      781 atgattgata atagccaatt tagagaggat ttatattatc gattaaatac aattccaata
      841 aatatacctc ctctaagaga aagaaaagaa gacatattaa ttattatgga agatttaatt
      901 aataagaaat tagtaatcac accagaagca aaaaaactta tacaaaacta tatgtggaaa
      961 ggtaatataa gagaactcca aaatgttaca tcctatttaa acattatgtg tgaagatatt
     1021 gtattagaga aagatttacc tccaaactta aggtcttcag ataacaaaaa tacatcatta
     1081 aaactaaaat atagtaagaa tgatatattg aatatattag agatactaat tttaaataag
     1141 gaatcagatg ttggtattgg taggggatta attttaaaag cactactcga taagaacctt
     1201 caaattacag aaggaaaaat taaaaaaata tttgaatatc taaaaaaaga agaacttata
     1261 atttgtagtt caggaagata tggaagtaaa ataactcaaa aaggagaaga tttttacaat
     1321 aaattaaaat ataaaggtct ataaaattat aatttatcaa acaaagaaat ctattcagga
     1381 ttaaagtact ccttcaatag gtttttttat ttttcaagta ccctaaaatt ataatttatt
     1441 ttgtacataa ataataggaa ttaataggaa taggataatt
//
LOCUS       DACTGW010000376         1470 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.384, whole genome shotgun sequence.
ACCESSION   DACTGW010000376 DACTGW010000000
VERSION     DACTGW010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.384"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(72..287)
                     /locus_tag="I9573_003622"
     CDS             complement(72..287)
                     /locus_tag="I9573_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798204.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVREGSSWAFNFIVQP"
     gene            complement(470..814)
                     /locus_tag="I9573_003623"
     CDS             complement(470..814)
                     /locus_tag="I9573_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798205.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            941..1324
                     /locus_tag="I9573_003624"
     CDS             941..1324
                     /locus_tag="I9573_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798206.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
BASE COUNT          530 a          196 c          191 g          553 t
ORIGIN      
        1 aattaattta cattctctaa ttttaaagta tatgtatatc tttgatttta ataaaattca
       61 cttaaaatat tttaaggttg aacaatgaaa ttgaatgccc aagaacttcc ttctcttact
      121 aatgttgcaa ttaattcaaa cccacaattg tattgaactg aatctctacc tactggtttc
      181 caactagaag gtgcattata tgagaaagaa tttgatgttg gctctttctt ttgttgctgc
      241 ttaattccta gcccattaga aataaatcca gtttgattta tatgcaccct tctatttgtt
      301 acacgaatct ggccgtctgc aatagaccag tttccagaaa ctttactaac ggaaatatat
      361 tgaaatgctc catctccttt tgatattgta tatgtaactg taccagatgc ttttacacca
      421 ccagttttat cccatccatc ttctgatata cttgcttttc taagaggctt tattgtatta
      481 gtatcaaaaa aactgtctta gaatatatct ttgatgttaa atttttagat tgtttaactt
      541 ctaataattg agttgttgat atagaatcaa cgtatatgga ctctcctgtt cttgcatttt
      601 tgattatacc tttttcattg gaacttttaa catcagtaat tccattttta gccatatcgt
      661 ataatttgtc catgtctgtt acttgtttct ctgaatacat tactttcata gatgaatctt
      721 ctaccttact agcacttgca tttgttggta atagtattgt cattatgagt aaagataaaa
      781 tagttgttac agattttttt aacctataat tcatattaat ccttactttt tatttgttat
      841 aacagacatt ctattgtaat agaatataaa tccacaatat ttttaaaatt agtattgaat
      901 aactattctc atgttataat tattcaaagg agggattgat atgaggaaaa aattttttat
      961 aggtattctt atagttttaa taatctctgt gatagctata cttaatataa aaaatgataa
     1021 aaatgtcttt attggagaat cagaaaattg gaaagctaaa tttgtaacta ctgaaggaca
     1081 ctcaaaatta gaaataaaat ataaaaatag taaagatgta gttgaacaaa aatttaatta
     1141 ttcctatcaa tttatagatg gtgaagatgc agttgtagtt gtaaaaaatg aaattttaga
     1201 agcaggtcaa gaaaaagcaa taaaaataca aagaccaggt ctttattcaa atagagaatc
     1261 tagttacaag ctaattatat cttggaatgg aaatgaggaa actataaact tgaaaaagaa
     1321 ataaccatat tatcaatttt taaaaaatta ttattcaaat tcttataaaa agagtgttta
     1381 gaaaaatgaa acactctttt ttgtataagt ttttgaaaac tataaatgaa atatatcaaa
     1441 atcgttcgaa aaattactga aataaactct
//
LOCUS       DACTGW010000377         1441 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.385, whole genome shotgun sequence.
ACCESSION   DACTGW010000377 DACTGW010000000
VERSION     DACTGW010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.385"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            12..776
                     /locus_tag="I9573_003625"
     CDS             12..776
                     /locus_tag="I9573_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAT4798207.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKAPTPDIVDILSEFNVNVL"
     gene            complement(1065..1286)
                     /locus_tag="I9573_003626"
     CDS             complement(1065..1286)
                     /locus_tag="I9573_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798208.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT          534 a          182 c          163 g          562 t
ORIGIN      
        1 agaggtagaa aatgtttgca gaagaaagaa tccaagctat attaaatatc ctaaaaaaag
       61 aaggtagagt tactgtcaaa gagctcagtt ctaaatttaa tgttacagaa gattgtataa
      121 gaaaagattt aaaaaatata gaaaagattt cttcaataag aagaatttat gggggagctg
      181 tactagctag agaaacacta gagaatcagg atacaaaaga cagaaaagaa attaatattc
      241 caacaaagaa aataatagca gaaaaagcat ttaatttaat tgaagataga gaaactatat
      301 ttttagatat ctctacaatt aacatcttgc ttgcaaagtt gttagctgaa agtaataaaa
      361 aagtaacact tgttacaaat atgttggata tactaaatgt agttacttca aaaccaaata
      421 atttaaatat aatttccact ggaggtttat taaatctcag tttagacggt tttgtcggta
      481 ctccaactat agagtttata tcgaaatata agtttgataa gacttttatg ggaagttgtg
      541 gagtagatgt attcaactct tcacttacaa cttttgaaat tgaagatggt ctcacgaaga
      601 aggctataat aaattcaagt aaaaaagtat ttatagttat ggaagataaa aagtttaaat
      661 ttgatggaaa tttcaagttt gctcatttag aagatattag ttctataatt acagaaaaag
      721 cgcctactcc tgatatagta gatattttat cagaatttaa tgtaaatgtt ttataagttt
      781 aattatatat tttataggat ttcttataaa tataaatttt tatttgataa ctattcttaa
      841 aaacatattt ctataatatt tattttatcc ttagtatcta ttttttaaaa tctagttcta
      901 taacatctac tttatcctta tgtctattta taaaactaat ttgaaataga ctttctaaat
      961 ctaatacagt tatttttaga aagtctattt ccttaaataa actatttaat attactggct
     1021 actttttata tgaatacaat aatttgttat tcataaataa aagcttattt catttcattc
     1081 ttcatttgct tctctaattc tttagaaaat ttcttttgtt catatctttc catcatctca
     1141 tcttttttca tttctaagtt taccatagca ccttgtgcat catctttcaa atcttcagct
     1201 gtatttttca taacataagc tgtcttttta acaccttctt ttaaatcttc tttcatattt
     1261 ttcatatctc tttttaatct atccatcaaa actacctcct ctgtaaatat aatacttcct
     1321 atatttattt ttcccaaagt acatatattt atttgtaata tcaattataa tttttaagtc
     1381 ttaaatatag tttaaactgt ctaaatctat acatttcaat aaattaaata ttttttattt
     1441 c
//
LOCUS       DACTGW010000378         1441 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.386, whole genome shotgun sequence.
ACCESSION   DACTGW010000378 DACTGW010000000
VERSION     DACTGW010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.386"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            21..755
                     /locus_tag="I9573_003627"
     CDS             21..755
                     /locus_tag="I9573_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAT4798209.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(897..1388)
                     /locus_tag="I9573_003628"
     CDS             complement(897..1388)
                     /locus_tag="I9573_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAT4798210.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          521 a          180 c          218 g          522 t
ORIGIN      
        1 tttaaaatgg aggaaacttt atgaaacaaa ataaatatga tgatgataga ttttttaata
       61 aatacagtaa aatggaacgt tctacaaatg gattagcagg tgctggagaa tggcatgttt
      121 taaaaaaaat gttacctgat ttcaaaggaa aaagagtttt agatttagga tgtggatttg
      181 gatggcattg tcaatatgca gttgagaatg gagcaatttc tgctgttgga gttgatattt
      241 cagaaaaaat gttaaaagaa gctagaaaca aaacgcaact tgataatata aattacatat
      301 gtatgcctat tgaagatatt aattttccaa aagatagttt tgatgtagtt attagttcct
      361 tggcatttca ttatattcaa tcatttgaag atatatgtaa gaatataagt aattgtcttt
      421 caaataaagg taattttgta ttttctgttg aacatcctat atttactgca tatggaagtc
      481 aagagtggta ctgtgataaa gatggcaata atctacactg gccagtagat agatattttg
      541 aagaaggagt acgtaagtca aattttctag gagaagaggt tataaaatat cataaaacat
      601 taacaactta tttaaatacg ttaatgaaaa caggatttga aatattagaa attattgaac
      661 ctcaaccaga agaaaaactg ttagatacaa ttcctgatat gaaagatgag ctacgtagac
      721 ctatgatgtt acttgtatct gcaaaaaaaa gataagtaaa tataaataat cattattgta
      781 gaaaaaaatt gtagaaaata attacactaa tggagatgat tataccagtg gtattaatta
      841 caccagtagt aaggaggcaa aagcattcgt tgcttttgcc attttttaag ttgcaattat
      901 acttctacgt agtatatttc gtttccattt tcttctataa gctttttcat atcttcaaat
      961 tttaaaaata cagtagcagt attatcattt ggatgtatac ctattttatc catttttaat
     1021 aaatccttat caaatacaac ttttacaaca gcctctttat catttaatat accaaaagga
     1081 gtcacagctc ctttagtaag tttcaagtat ttttgaagtc tttcttctga tgcaaaacta
     1141 agaggtttac tatcaatttg ttgacgaata tcttttaaat ctgctttttt gtctttacct
     1201 aatacaacta aaaaatgttc ttttccttta gcatcacgaa gaaataggtt ttttacaaca
     1261 aattccatat gtaatgtttt taattcttcc atttcttcaa ttgtataaac tggtggatga
     1321 tttacagcct cataaggtat gcttaactca tctaattttt tatatacttc ctgttgttga
     1381 ttgttcatga tttcctccca ttagaaattt attttatata taaataatat tatcacagaa
     1441 a
//
LOCUS       DACTGW010000379         1346 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.387, whole genome shotgun sequence.
ACCESSION   DACTGW010000379 DACTGW010000000
VERSION     DACTGW010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.387"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            128..>1346
                     /locus_tag="I9573_003629"
     CDS             128..>1346
                     /locus_tag="I9573_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798211.1"
                     /translation="MLNKKNISSIIMTMILSLSTTVFVFADENTNVKGQDVTKYNEEM
                     VSKNLEDLSIEQLIDIRDKAIDKNLEGLRKFGEDSVKERKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVEYENSINDIMDPSGDFFKVTNQSYQINEFVLKNGKNYKEWSY
                     EYVEFLIEHLEDLCNFVDFDAKYVIEMVDPSIKPDLSDNQKKSLNNELIKMYSSNTLS
                     TNIKDEIENWENNLNINEKNSNEPLPRVSPSSYRQQALAYAKKHGYTNGYYGSTNRKN
                     NAPSPYYNFQKDGYGDCANFVSQCLHEAGVTFWTNNNPWYYYSTSNRSPSWAGAREFK
                     IHWMNRIAYQTLTVSNSLSFLRPATPVSILNSSGVATHTLISTDKHSNGYNFSYAAHS
                     DEGARTNLLKKLEG"
BASE COUNT          557 a          142 c          212 g          435 t
ORIGIN      
        1 attatattat gatatttagt aaacgctctt ttagaaaaaa acggtgataa acttgtttta
       61 aattttagta gaatatgatt tttgttttgg caagtattaa tacatatatt aaagagggag
      121 gtttaatatg ttaaataaaa aaaatataag ttcaattatt atgacaatga ttttaagctt
      181 atcaactact gtatttgttt tcgctgatga aaatactaat gtgaaaggac aagatgtaac
      241 caaatataat gaagaaatgg tctctaaaaa cttagaagat ttaagtatag aacaattaat
      301 agatattaga gataaagcta ttgataaaaa tttagaaggt cttagaaaat ttggagaaga
      361 ttcagtgaaa gaacgtaaag attcaatttt aaactttgaa tttggattag tagcttttgt
      421 taacaaaaat tatagtaact taacagaaaa agagaaatta ataagacaaa tcaatattgt
      481 tgagtatgaa aattctataa atgatataat ggatccttca ggcgattttt tcaaagtaac
      541 aaaccaaagt tatcagatta atgaatttgt attaaaaaat ggaaaaaact ataaagagtg
      601 gtcgtatgaa tatgtagagt ttttgattga gcatttagag gatttatgta attttgtaga
      661 ttttgatgca aaatatgtaa tagaaatggt tgatccttca ataaaaccag atctttcaga
      721 taatcaaaag aaatctctaa ataatgaatt aataaaaatg tattcaagta atactctaag
      781 tacaaatata aaagatgaaa tagaaaactg ggaaaataat ttaaatatta atgagaaaaa
      841 ttcaaatgaa cctttaccaa gagtaagccc ttctagttat agacaacaag cattagctta
      901 tgctaaaaaa catggctata ctaatggtta ttatggtagt acaaatagaa aaaataatgc
      961 accttctcca tattataatt ttcaaaaaga tggatatgga gattgtgcta actttgtttc
     1021 acaatgctta catgaagctg gagtaacatt ctggacgaat aataatccat ggtattatta
     1081 ttctactagt aatagatcac cttcttgggc tggagcaaga gaatttaaaa tacattggat
     1141 gaatcgaatt gcatatcaaa cacttactgt atcaaactct ttaagttttt taagaccagc
     1201 aacacctgta agtatattaa atagtagtgg agtagcaact catacactaa tatctacaga
     1261 taagcattca aatggatata atttttcata tgctgcacat tcagatgaag gtgcaagaac
     1321 aaatttgtta aaaaaacttg aaggaa
//
LOCUS       DACTGW010000380         1344 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.388, whole genome shotgun sequence.
ACCESSION   DACTGW010000380 DACTGW010000000
VERSION     DACTGW010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.388"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            99..>1344
                     /locus_tag="I9573_003630"
     CDS             99..>1344
                     /locus_tag="I9573_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAT4798212.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKVDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGP"
BASE COUNT          576 a          168 c          159 g          441 t
ORIGIN      
        1 tttacgtttt ttaattttat aattaaaaat ttaaatttta tacatattat aataaattgt
       61 gatatatata aaataattaa agataaaagg agtattttat gaacatactt ggattaatag
      121 ttgaatataa cccatttcat aatggacaca tatatcattt atctaaatct atagaaaaaa
      181 caaatgcaac acatactgta gcaataatga gtggaaattt tctacaaaga ggcgaaccag
      241 ctttatttga taaatatact agagcaaaaa ttgctgtcga aaatggtgta gacttagtta
      301 tagaattacc aactttattt gcttgtcaaa gtgctgaaat tttttctcat ggtgctataa
      361 caattttaaa ttcactaaac tgcataaatt ctatatgttt tggtagtgaa gaaggaaata
      421 cagaaattct ttataatata gctgaaattt tagcaaatga accaaatgaa tttaagttac
      481 ttttaaaaaa atatttagat gatggcctgt tatttccaac agctagagct aatgcacttt
      541 ttgattatat acatcaagac aaaaaaaata attttataca aaattattca aaggaacaat
      601 tatcaaaaac attaaattca tcaaacaaca tactgggtat agaatacata aaaaacctca
      661 tccatttaaa cagtagtata aaaccattta ctatctccag ggtccaatca gaatataatt
      721 ccgaagaaat tgagagtgat atttgttctg ctactgctat aaggacttca cttaaagaaa
      781 atacagacct ttttcattta aaaaatgtta ttccagaaaa aacttataac atcctaaatg
      841 aaaaagtaga taatggtttt ttacctatgt ttgatgactt cttctttgat attttaaaaa
      901 caattatact tagagatatg gacactttaa aagagtattt tgaagtaaat gaaggcatag
      961 aaaacagaat ctataaagat atctttacat ctgaatttct acatgagcta caattagcaa
     1021 ttaaatccaa gcgatacact ttaaccaaaa tcaaacgaac tctaaacaat atattgttag
     1081 gtattaaaaa aagcgacatg caacttataa aaaacataga tactatacct tatataagaa
     1141 tacttgcttt taatgataaa ggtagagaaa ttctaaaaga gataaaaaat aaatctgaaa
     1201 taaaaataat aaacaagttt tcaaatatat cattttcatt agatgatacg atttttaaaa
     1261 cactcataag ttatgatata aaagctacaa atatatataa ttcaatatat tataaaaaca
     1321 ataaagattt agtaaaaggc ccta
//
LOCUS       DACTGW010000381         1335 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.389, whole genome shotgun sequence.
ACCESSION   DACTGW010000381 DACTGW010000000
VERSION     DACTGW010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.389"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            150..395
                     /locus_tag="I9573_003631"
     CDS             150..395
                     /locus_tag="I9573_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HAT4798213.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(612..881)
                     /locus_tag="I9573_003632"
     CDS             complement(612..881)
                     /locus_tag="I9573_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HAT4798214.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIGQCRKWLEETVSN"
     gene            complement(1093..1317)
                     /locus_tag="I9573_003633"
     CDS             complement(1093..1317)
                     /locus_tag="I9573_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798215.1"
                     /translation="MKYSKGFKLIYSLVEYSKRFKLIYDVMKYSKEFKLIYSLVEYSK
                     RFKVIYSVMKYSKRFKAIYNAVKYIQGDLK"
BASE COUNT          542 a          199 c          155 g          439 t
ORIGIN      
        1 atgattcagt actttagagg tggtaaaata tagatagatg aaagagcata aataaagtga
       61 tataataaaa aattaaatat taactataat aaaaaaatat ctggtactaa gtataataca
      121 aaaataatat atcagaaaga aggtgaagta tgggagaact acaaaaaatt ccaggggtag
      181 ggaaagcaac agaaaaatct ttaatcatgt taggatatac aactattaaa tcacttaaag
      241 atgcaaaccc agcacaaatg tatgaaaagg aatgtttaat gcgtggacag cacatagaca
      301 gatgtcagtt atatgtatac agatgtgcag tatattttgc atcaactgaa aatccagaac
      361 cagaaaaatt aaagtggtgg tattggaaag attaaaaaat caaaaaagtt agatataaaa
      421 aagagttgaa tatccgttta aataataaaa actaatactc aactcttttt tagtcgtaaa
      481 ttttatgtaa atctatttgt atggctaaat atacccatca ataaatgtat aaatatacca
      541 atcaataaat gactaaacat actacctaac aaaggattaa ataaattact taacaaagca
      601 ttctaaaaca attaattaga aactgtttct tcaagccatt ttctacattg cccaatattt
      661 accatagggt caaagccttg gtcttttccc ttatttaaaa gcatatcaca agggaaagta
      721 tcacattcac cacaatgatt aagttcctta ctttcacagc aagttttaac cttacattct
      781 ccaccccaaa atggtttttc catagttggg caaccagtgc aatttacttg ttcttttttt
      841 gtacaactgt cacaacagac accacatctt gattcaaaca tgttcaaaca tcctttccat
      901 tatttaaaaa tattataaaa tgattttaga tgactacaaa agtgcaaata gttgatttta
      961 agtcatctaa atgttaaaca taaaattatt aatactggta aatttataaa aactattcca
     1021 aaaatatttt tgaatatttc aaaacacaat aaattacttc aaatcccttt gaatattcca
     1081 taacactata gattacttta aatccccttg aatatatttc acagcattat agattgcttt
     1141 aaatctcttt gaatatttca taacactata gattacttta aatctctttg aatattctac
     1201 aagactatag attagtttaa attcctttga atatttcata acatcataaa ttagtttaaa
     1261 tctctttgaa tattctacaa gactatagat tagtttaaat ccctttgaat atttcataac
     1321 actatagatt acttt
//
LOCUS       DACTGW010000382         1321 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.390, whole genome shotgun sequence.
ACCESSION   DACTGW010000382 DACTGW010000000
VERSION     DACTGW010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.390"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..140)
                     /locus_tag="I9573_003634"
     CDS             complement(<1..140)
                     /locus_tag="I9573_003634"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798216.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIV"
     gene            complement(653..>1321)
                     /locus_tag="I9573_003635"
     CDS             complement(653..>1321)
                     /locus_tag="I9573_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAT4798217.1"
                     /translation="KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGG
                     SDTGTTGKPLGGIKEKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNS
                     LRPDLFTSIHYNASDTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAK
                     TRTLSTDPTKDYLYVLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIE
                     DSLK"
BASE COUNT          435 a          219 c          140 g          527 t
ORIGIN      
        1 acgatacttt ttttctctaa gtttatattt aaatcagcgt tattttccat agcattagat
       61 tcagtagcaa atgccattgt aggtgcgacg attgccattg aaagaatccc tgttgttaat
      121 aattttgtat ttcttctcat attaatctcc ttacttaaca taaatttcta ctttattttt
      181 aatcttttaa cttttgaaaa tatgtcaaag tgtatctaac ccaaaatata aataatatat
      241 aagtaatata taaaaataaa cttataaaac tcaaaaaata aaaaaattaa atatatttaa
      301 tacatttaat tatctatact atatcattta ttgacataaa aatccacaaa aaacacacaa
      361 acacacaaac acacaataaa atatagtaat taagctttta ttgtgtatta caaatgaata
      421 ctccaactct tgacaattaa ctgtagcctt aagatatgta aattcttcat ataatataaa
      481 tccttttaca attaatcata aaaagactgt atatacctca tttcgcactt atatgcacaa
      541 tgtatataca gtctttatta ctattataat tacattctta tcttatagct ttagtttaaa
      601 tttaaaattg cagattaatt tattttaaat ttttctatgc tctcattgta gactatttta
      661 atgaatcttc tatgccttta cctatttgtg tacccatttc ttttacttta tttgatgtat
      721 taagtaaact catatctttc tcattatcca aaaatgcaca ttctacaagt acagctggca
      781 tatcattatt tcttaaaaca tataaataat cttttgtagg gtcagttgat agtgttcttg
      841 tttttgcacc tctatttttt agattaaatt tctctaagat tctatttaat atatttgtag
      901 ctacagtttt agtagtccct ccatctttat ctttaagctt ataaaatact tctacaccat
      961 tcccagttgt atcagatgca ttatagtgta tactcgtaaa taaatctggt ctcaaagagt
     1021 tagataaagc agttctattt ccaagagata aagtcttatc tgtgtctctt gtcattatta
     1081 cattgaatcc ctttgaacgt aaatattcag ttgtcgcaag tgaagtattt aatgtatagt
     1141 ctttttcttt aataccccct aatggtttac ctgttgttcc agtgtccgaa ccaccatgtc
     1201 ctgggtcaat cataactgtc ttacccccac tatttcctgg ttctgttgga gttgtattgt
     1261 gtttagataa tgatgatgct atagagttca ttaccttaga agtcaatcct cctcctattt
     1321 t
//
LOCUS       DACTGW010000383         1320 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.391, whole genome shotgun sequence.
ACCESSION   DACTGW010000383 DACTGW010000000
VERSION     DACTGW010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.391"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            67..1281
                     /gene="dpaL"
                     /locus_tag="I9573_003636"
     CDS             67..1281
                     /gene="dpaL"
                     /locus_tag="I9573_003636"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HAT4798218.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLRDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
BASE COUNT          533 a          158 c          282 g          347 t
ORIGIN      
        1 actataagac acaaagataa aaaattaaaa tgacaccgaa aaatttgaaa ggggaatata
       61 aaataaatga gagaaataaa atggaaatta aataacttac ctaaatcaga ggaaaaagaa
      121 aaaggaattg agtttttaag agatgaagaa attgcaaagg ctaaagcttt tcatgagagc
      181 tttccacaat atgaaaaaac tcctttagtt aaattagata acttagcaaa atcactaggc
      241 gtaagcggaa tatatgttaa agacgaatct tatagatttg gtttaaacgc atttaaagtt
      301 ttaggtggtt cttactctat gggtagatac ttagctcaaa gattagatac agatatatct
      361 gagttagggt atgacaaatt aacttcaaaa gaaataaaag aaaaattagg agaaataact
      421 ttctttacag ccacagatgg aaatcatggt agaggagttg cttggacagc taataaatta
      481 ggtcaaaaat ctgttgtatt aatgccaaaa ggttcttctg aatttagatt aaataaaatc
      541 aaaggagaag gtgcagatgc tagtataaca gacttaaact atgatgatgc agtaagatta
      601 gctaatgatt atgcagaagc tgatgaccat ggagtaatgg ttcaagatac agcatgggac
      661 ggatatgaag aaataccagc atggataatg caaggatatg gaactatggc taaagaagct
      721 atagaacaat taaaagaata tggagtagat agaccaacac acgtatttgt tcaagcagga
      781 gttggttcac ttgctggtgc agttcaaggg tatgttgctt caatatatga tgaatgccca
      841 ataacagtag tagtagaggc tgatgaggct gattgttact ataaatcagc agaagctgga
      901 gatggaaaac caagatttgt tggaggagat atgccaacta taatggcagg gcttgcttgt
      961 ggagaaccaa acactatagg ttttgaagtg ttaaaaaatt attcatcagc attcgtttca
     1021 gcaccagact gggtatctgc taaaggtatg agaatattag gaaaccctct aagaggagat
     1081 gagaaagtaa tctctggaga atctggagca gttacaactg gagcattagt ttcaatcctt
     1141 gaaagtgaag atttaaaaga cttaagagaa gctttgaaac tagatgagaa ctctaaagta
     1201 ttattaataa gtacagaagg agatactgac cctgataaat atagagatat agtttggaat
     1261 ggtgaatgcc aaagtaaata aaagacttaa ggaaaataaa aacttaacaa ataaatgaaa
//
LOCUS       DACTGW010000384         1318 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.392, whole genome shotgun sequence.
ACCESSION   DACTGW010000384 DACTGW010000000
VERSION     DACTGW010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.392"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(218..484)
                     /locus_tag="I9573_003637"
     CDS             complement(218..484)
                     /locus_tag="I9573_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HAT4798219.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTDIEKPFWGEQCPVKSCCED
                     KNLIHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(527..1282)
                     /locus_tag="I9573_003638"
     CDS             complement(527..1282)
                     /locus_tag="I9573_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAT4798220.1"
                     /translation="MPHKLLRQYIAHYTISIPDTSIKEKLTLIPDASGCMIFKFDKKG
                     IESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTGLSQRESTDLKISLEDFNT
                     LFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMTIPILENARKQNSIMSVKN
                     ISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEVQNNKRPFATLAQDIGYYD
                     QSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT          456 a          191 c          154 g          517 t
ORIGIN      
        1 agtctatttt aattcaattt tgtaacatat attttaaata gacagagatt gagattttat
       61 atttctaatt ttagtaatca gattttatca ataaattact attctattaa tttaattaaa
      121 taacctttaa atttaattat aaaaactgtt gcagaaattt ctacaacagt ttaaaaaatt
      181 taccttacaa aaacttattt tataacaaga gagtatatta taaggctagt acagcccatt
      241 ttttgcactg agaaattctt ttgccatcat caccttgttc tttgtcatat gcaaattgat
      301 taagcatttc acatggaaat gtatcacata gaccacaatg tataaggttt ttatcttcac
      361 aacaagattt tacaggacat tgttcacccc aaaatggttt ctcaatatct gtacaacctt
      421 tacaattgac tttttccttg tatccacatt cataacataa aattccacat cttgattcta
      481 tcattttaag tcactcctta aatataaatt ttattatgat tgtatatcaa aatttatact
      541 tttcattgta aaaatcggac aaatttttta tataggttgt tggattaaca ccacaaatag
      601 atttaaaatc atttataaaa tgagattggt cgtaataacc aatatcttgt gctaaagttg
      661 caaatggtct tttattgttt tgtacttctt gtaatacaag gtttatacga agtaatctaa
      721 ggtatgattt aacactcatc ccaagagaat tattaaaaat tctatttaaa tgtcgttcac
      781 tataacaact aatttgtgaa atgtttttaa cagacataat agagttctgt tttctagcat
      841 tttcaagaat tggtatagtc atatctacta tatttgattt caataagtat gataagaata
      901 acatatctaa ttgttctact aattccttta tagtacttgt tctttcaaaa atggaattaa
      961 cctctaagct aaataatgtg ttaaaatctt caagtgaaat ttttaaatct gtagattctc
     1021 tttgagaaag acctgtcaaa taatatactc cacctgggcg aaactcaacg aatactctaa
     1081 aaagtacatt ttctatatca ttttttacaa tagtagtttt tgttgtggct ccccagaatg
     1141 cgctttctat accttttttg tcaaatttaa atatcataca tccactagca tcaggtatta
     1201 atgtaagctt ttctttaata cttgtatctg gaatagatat tgtatagtgt gctatatact
     1261 gtcttaacaa tttatgtggc aaaatatatg tatactgttt gcaggtattt aaaatgtt
//
LOCUS       DACTGW010000385         1266 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.394, whole genome shotgun sequence.
ACCESSION   DACTGW010000385 DACTGW010000000
VERSION     DACTGW010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.394"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(106..597)
                     /locus_tag="I9573_003639"
     CDS             complement(106..597)
                     /locus_tag="I9573_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HAT4798221.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDENIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          470 a          178 c          105 g          513 t
ORIGIN      
        1 tatattttaa aaggctaata taatatgtat tttcttctat acaatcaaaa ggcttcatat
       61 ctaagatatg ttatgacact aatattcaca attttttagt acttatcaca ttataaaaaa
      121 tattacagct agtccatcat cagatgcttc ttgtataaac tttttaagct caattagatt
      181 tgcccataca tattcaaaaa aatcatcttc actctcatca ttcataacag gataaatatt
      241 attttctaac atctcattaa tattaaatct ttttctaaat gcttgttctt ccaaactgtt
      301 cattgctttt gatagttttt ttacatcatt tgctgtaatc aacatgacag gaaattctcc
      361 atcaatttcc ataagtatgt ttccacttag tactaatttg ctaaatatat tctcatcatc
      421 tccaccaaaa ggacaacctg tcaaagtaaa gtgaatggca tgccatgctt tatcaatatt
      481 gatagtacta ttttcgttgg catcttcaaa aacaaaatct tgtaaatttt cttctgcttg
      541 ctgtaattta aaaacattct cttcacttat tttagtataa catcctatca tccccataat
      601 tacattcctc ctaagtagcg tttttttaat tgtaacttat aattaaactt agttacaata
      661 aaatatattg agaaaagtat tatttcatct taaatttaaa tacctatatt atcaactaaa
      721 attctgtgtt tatagcgtaa gtattattat tttttcctaa aaattaaatg aatcagaagt
      781 tctataggca atattgacta taaataagaa ctattacttt taaaaaaaat ttaagaaatt
      841 atactgtaga atcatgctct acttattagc ttcaaataaa aaataaatca tgttctcaaa
      901 ttaaataata aataacaata aatttaaatt ttaaactata agagtttctg aagtgttaat
      961 aagcatacca ataattatat gtgtaagaat ttgatttaat agatatgaat aatttttaca
     1021 ttaattataa atatattaac ctattattat aaattctata aaaagaaaag ttatcttaaa
     1081 taaaaaataa gtgtatctta aattttaaat gatacactta tttttatatt gaaattatat
     1141 tttttaatat ctattcttaa ttaggtattt ttaaattatc tgtttacatt aaatatctct
     1201 taattactta tctttcagct agcttcttct attaaatgtt tctcaattac ttatttttca
     1261 attagc
//
LOCUS       DACTGW010000386         1265 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.395, whole genome shotgun sequence.
ACCESSION   DACTGW010000386 DACTGW010000000
VERSION     DACTGW010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.395"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1189
                     /locus_tag="I9573_003640"
     CDS             <1..1189
                     /locus_tag="I9573_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4798222.1"
                     /translation="ITNLLIKALYQIKEHILINEEKMNHFLVRGFVEMVVFRVNKRFE
                     YEICQSKFAKKEIIDSRVKMTLIHEAFFTIFSLIVTFIKMLIIVYSWKTKTISIGSII
                     ALITLLDNAYTPIAIFNVLYIQFKLDKAAYKRYTDILELNNDGQLLAGKEVNLMYGNI
                     EFNEISFLYNNKREIFNKLSLSIKSGEKVAIVGESGSGKSTLIKLLIGLLKPSEGTIF
                     IDDYNLLEMNLNSYYNYVTYVSQDSPIFDGTLRENIVFGKTIKSNDIIEVLEKVGLKN
                     LYLKLEAGLDTKLGEKGTTLSGGEKQRLALARLWFSDAKIIILDEATSALDNITEEKV
                     MLNVMEFLKDKTTIMIAHRFNSIKNVENIIVLKEGSIIGSGSFKKLLDGNLYFRELYY
                     TMS"
BASE COUNT          498 a          110 c          210 g          447 t
ORIGIN      
        1 tattactaat ttactaataa aagctctata tcaaatcaaa gagcatatat taattaatga
       61 agagaagatg aatcattttt tagttcgtgg atttgtagaa atggttgtat ttcgggtaaa
      121 caaaagattt gagtatgaaa tttgtcagtc aaaatttgcg aaaaaagaaa ttattgattc
      181 tagggttaaa atgacactga ttcatgaagc tttcttcacg attttttcat taatagtgac
      241 gttcataaaa atgttaataa tagtttatag ttggaaaact aaaacaataa gtataggttc
      301 tataattgca ttgataactt tactagataa tgcatatact cctatagcta tttttaatgt
      361 attgtatatt caatttaaac tagataaagc agcatataaa cgctatacag atattttgga
      421 acttaataat gatggtcaac tgttagcagg taaagaagtt aatttaatgt atggaaatat
      481 tgaatttaat gagattagct tcttgtataa taataagcga gagatattta ataaacttag
      541 tttaagtatt aaaagtggag aaaaagtagc tattgttgga gagagtggtt ctggtaaatc
      601 aactttaatt aaactattaa ttggattatt aaagcccagc gaaggaacta tatttataga
      661 tgattataat ctacttgaga tgaatctaaa tagttactat aattatgtta catatgtgtc
      721 acaggattca cctatatttg atggaacttt acgtgagaat attgtatttg gaaaaactat
      781 aaaaagtaat gatattattg aagtcttaga aaaagtaggt ttaaaaaatt tatacttaaa
      841 attagaagct ggtttagata ctaaattagg tgaaaaagga actacattat caggtggaga
      901 aaagcagagg ctagcattag caaggctttg gttttcagat gctaagataa ttatattaga
      961 tgaagctaca tcagctttag acaatattac agaggaaaaa gttatgctaa atgttatgga
     1021 atttttgaaa gataaaacaa ctattatgat tgctcacaga tttaactcaa ttaagaatgt
     1081 tgaaaatata attgttctta aagaggggag tataataggt agtggtagct ttaaaaaact
     1141 attagatggt aatttatatt ttagagaatt atactataca atgtcataat tattatgtaa
     1201 gtataagaaa atatttttct taatttaata ttatttaaat attactggaa tattattaaa
     1261 aattt
//
LOCUS       DACTGW010000387         1244 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.396, whole genome shotgun sequence.
ACCESSION   DACTGW010000387 DACTGW010000000
VERSION     DACTGW010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.396"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            627..>1244
                     /locus_tag="I9573_003641"
     CDS             627..>1244
                     /locus_tag="I9573_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HAT4798223.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNI"
BASE COUNT          506 a          144 c          173 g          421 t
ORIGIN      
        1 tttttaccta aatttatttt aacattctat aattttcaca atattcttta aattttactc
       61 atataaacaa tattattctg gatgctaata gaattaatct ataaatcata gtataattat
      121 gcagaaataa ctcgaatttc actacttata aagagattga atattaatga atctaaatca
      181 tttgcattac ttcagagttt tgactaaagt tgaacaccat acaaaagtag ctactcaatt
      241 gtttataccc caaccaagct taagtaatgt tatatagatt cttgaaaaag aatttggtat
      301 ctttttattt gaaaaacaag gtagaaatgt ttccctcact aaatacggaa gtttttttct
      361 aacctatgtt gataaagcat taggttaatt agaaaaagtt tacatataaa atatattaaa
      421 ttgttgcaag ttttattgtt ttgatataca atcaactaaa catataatct ttataaaata
      481 ctatacagtt aaaagtctgt tttaaaaaaa aatgtataaa ttataaatca aaatatagat
      541 actatcaagg agtgattctg tgaagagatg tattgtagta gtggaatagt caaaaacata
      601 atatatatta aaggagagat ttaaatatga ctattccaaa ttcaaataag gtatatccaa
      661 gaagtaatga ttatcaaact gtatatctta aaaatgtaat aacaagggaa aatatagaag
      721 ttggagatta cacaatatat aatgactttt ataatgaccc aagagatttt gagaaaaaca
      781 atgtattata tcattaccct ataaacaatg ataaattaat aattggtaag ttttgctcaa
      841 tagcatgtaa tgcaaaattt ctaatgacta gtggaaatca ctcaatgaaa tcaaaatcca
      901 catatacttt tcccatcttt tatgaagaat ggaaactcga tatcaaccat ataacggacg
      961 cttgggacaa taagggtaac attataattg gaaatgatgt gtggataggt tatgattcta
     1021 taattatgtc aggtgtaaat attggtgatg gtgcaattat tggaacaaga gcagtagtta
     1081 ctaatgacgt ctcaccgtat tctattgttg gaggagtacc tgcaaaaatt ataaaaaaga
     1141 gatttaataa tgatacaatt tcaaaattat tagaaattaa atggtgggat tggccatata
     1201 aaaaaattca atcaaacatt aagcatattc aatcaggaaa tatt
//
LOCUS       DACTGW010000388         1227 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.397, whole genome shotgun sequence.
ACCESSION   DACTGW010000388 DACTGW010000000
VERSION     DACTGW010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.397"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            137..505
                     /locus_tag="I9573_003642"
     CDS             137..505
                     /locus_tag="I9573_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT4798224.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KILSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
     gene            518..>1227
                     /locus_tag="I9573_003643"
     CDS             518..>1227
                     /locus_tag="I9573_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HAT4798225.1"
                     /translation="MELFIEVIKFNIYISLLILFFILFTKKISKYTFKFNYFICLLIV
                     VRMMFISKINLNILNINNTTINIYKHNLDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIHWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTS"
BASE COUNT          511 a          120 c          153 g          443 t
ORIGIN      
        1 atctttttta tctataaatc tatactactt agttctcaag cataaaataa tataaagatt
       61 ctttatacat gatgatatat tatattgact attagacatt tgtctaatat aatcaagtta
      121 aaggtgagtg attatattga aaaaaatacc aaaatccgag ttgttagtta tgaatttcat
      181 atgggataaa ggcatagata aaataccagc cagagaagta gctaattata tggaaatttc
      241 tcatggatgg acaaaaggta ctactggtaa aatactttct agattaagtg ataaaggttt
      301 tttagaattt gaaaaagatg gtagaaatac tttatacact attaaagtta aacatgagga
      361 atatctaaaa tttgaaacaa aggatttttt agagcttata cataaaaatt ctattcatag
      421 ctttatatca tcattaggtt gtggaaatga aatatcagat gatgacataa aagaacttga
      481 acaatggcta aaaaaaagaa aataggagac gtattttatg gaattattta tagaagttat
      541 taaatttaat atttacataa gtctactaat tctttttttc attttattta ctaaaaagat
      601 aagcaaatat acttttaagt ttaattattt tatatgtctt ttaatagtag tcagaatgat
      661 gtttatatct aaaataaatt taaatatatt aaatatcaat aatactacta ttaatatcta
      721 taaacataat ctcgataatt atataccata tgaccagggc attactttag atatatttat
      781 gtacatctgg atattaggaa ttatagcaat ctctttttac tatttaagaa caaattttaa
      841 attttataat tacatagaaa attttaaaaa agaaatttta gaaatagata ttactagtat
      901 cttagatgaa caagcaaaag aactaagtat aaataaaaaa cttcagatat ataaggttaa
      961 aggtatttat tcccctgcca ttataggttt aacacgctgt aaaataataa ttccagaaaa
     1021 aaagtacaat aataaagagt taagatttat ctttaaacat gaattaattc attataaaag
     1081 aaaagataac ttatttaagc ttctaattag tataatatgt atcatacatt ggttcaatcc
     1141 aattttatac ttattaaaaa aatattttca tgaattatgt gaattatctt gtgatgaaat
     1201 tgtgattgac aaatgtacca cgagtga
//
LOCUS       DACTGW010000389         1221 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.398, whole genome shotgun sequence.
ACCESSION   DACTGW010000389 DACTGW010000000
VERSION     DACTGW010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.398"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            128..1204
                     /locus_tag="I9573_003644"
     CDS             128..1204
                     /locus_tag="I9573_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HAT4798226.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT          480 a          173 c          214 g          354 t
ORIGIN      
        1 aaaataaatt atatataaaa aataaatttt atgttgacat agttaattca gcttgatatt
       61 atagttatta aaaataagga cagtaaaaac gctatccata ttacaaacta aaaagggggg
      121 aattataatg agtaagaaat tatttattcc tggtcctatt gatgttaaag aagaagtctt
      181 gcaaaagatg gctacaccaa tgataggaca tagaggaaaa gatgcatcta tgctccaaaa
      241 gagtataagt gaaaagatgc aaaaactatt ctatacaaac aacacgatac ttttatccac
      301 gtcatccgga actggtttaa tggaaggctc cattcgttcc tgtacatcca aaaaggcagc
      361 agtattttcc tgtggatcct ttggtgatag atggtacaaa atggcagtgg ctaataatgt
      421 cccagcggat atatttaaag ttgaattagg agaggcaaca acgccagaaa tggttgataa
      481 ggtgctttct accggagaat acgatttaat aactgtaact cacaatgaaa cttcaactgg
      541 aataagaaat ccaatagaag aaattggaga agttgttaaa aagtatgaag atgtaatcta
      601 ctgtgtagat actgtaagtt cagccggtgg aataaaagtc gaggttgata agattggaat
      661 agatatttgt ataacatcag tacaaaaagc actgggatta ccaccaggaa tgtctatttg
      721 cacattttct caaaaagcaa tcgatagagc taagcaagta ccttttagag gagtatatct
      781 tgatttgtta gcaatgtatg aatacttaat caagaaaaac taccaatacc cttcaacacc
      841 atctctttct cacatgttcg ctttagattt ccaattagat aatatattag atgaaggcct
      901 agataacaga ttcaacagac atgaagacat ggcaaactta gttagaaact gggctaaaaa
      961 acacttccaa atttttacca atgaaaatca cttatccaat acacttactg ttatagaaaa
     1021 tactcaaggt attagtgtat caaatttaaa ttcaaaatta caagaacgag gattccaaat
     1081 agcaaatggc tatggagatt taaaagaaaa aacttttaga atttcacata tgggtgacta
     1141 tactgtcgaa gatgtacaag aattattaga taatattgat gatatattag gttttaataa
     1201 ataatattgt aaaaatatgt a
//
LOCUS       DACTGW010000390         1191 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.399, whole genome shotgun sequence.
ACCESSION   DACTGW010000390 DACTGW010000000
VERSION     DACTGW010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.399"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            624..1175
                     /locus_tag="I9573_003645"
     CDS             624..1175
                     /locus_tag="I9573_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HAT4798227.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          474 a          125 c          199 g          393 t
ORIGIN      
        1 attaatataa gaagaattag agccaaagag cagactatta gaccattaaa gtaattattc
       61 atagaatttt gtatatgaaa agaaatgtat agtataattt acttttttta taaaaaatag
      121 attacaaaat atatttttta taaaaaggta ttgacatttt ttagaaaaaa gaatatcatt
      181 attttaacaa caaaattcaa tacaataaat taaatcctat gacaaggaag agtagttaaa
      241 tttaggttca gtatagagag ctgaggatgg tggaatctta gcaagaaaaa atttgatgaa
      301 tggcccttag aggaataaat tgaaatcata gttttgagag tagactttat cgtcagtcgc
      361 acgttatagc gaataaggta tgtaagtacc tgataatgag atgtatgaaa attgaatttt
      421 atattaattt ttatataatt taggtggcaa cgcggataat ctccgtccta ttagtttagg
      481 acggagtttt ttattttact gaaaatttaa aagatagaag gtggtgagaa agatgttatg
      541 aatttcaaaa gctaaagatg atgatatagt aggttggcaa aaaaataaat tgaaaattaa
      601 aaaaatagga ggaagtacaa aaaatgaaaa caaatacaaa aaaattaact ttaaatgcaa
      661 tacttttagc gatgggatta ttaatacatc aaatcacacc agctatagga ttaccaatga
      721 agccagatgt accactagca atgttatttg taatattggt tttaaataga gatgattata
      781 aaacttgttt gatagcgggt atagtaacag gaatatttac agcacttaca tcaagttttc
      841 cgggaggtca aattcctaat gtaatagata aaactcttac agcaaatata gtatttttat
      901 taatgaatat tagttataat atgcctttta taaaaaaatt agctaagaaa acacaagata
      961 ctgtagtagt tgcaataata atgccaatag gaactcttgt aagtggaact atctttttat
     1021 tagcagctca agctatagtt ggtcttccag gagcttcatt tacagcttta ttcttagcag
     1081 tagtattacc agctgtttta ataaatctag ttgcaggaat atttttattc aagattgtta
     1141 gtctatcaat aagaagagtt agttatcaag cttaactacg aaataatatt t
//
LOCUS       DACTGW010000391         1175 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.400, whole genome shotgun sequence.
ACCESSION   DACTGW010000391 DACTGW010000000
VERSION     DACTGW010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.400"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            380..1030
                     /locus_tag="I9573_003646"
     CDS             380..1030
                     /locus_tag="I9573_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4798228.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
BASE COUNT          506 a          116 c          187 g          366 t
ORIGIN      
        1 aatttgaagt acaacgattt aatctatagt acactagcat gatttgaagt ataacgattt
       61 aatctatagt acaataactt gatttgcaat acaataattt aatctatagt ataatagcat
      121 gatttgaagt ataacgattt aatctatagt acaataactt gatttgaagt acaacgattt
      181 aatctatagt acaataactt gatttgcaat acaatgattt aatctatagt ataatagcat
      241 gatttacagt acaataattt aaattgtagt acaaaagtac tatcaaataa gaatttctac
      301 tttaaaaata ttactaaaag taattaaaat ccaataattc aatataatat ggcaaaagaa
      361 tagaaataag gtgagagtag tgaaagtaaa gtcaaattta gatgaaattt tatatcataa
      421 gattattgaa agtcttattc gtggtgaata ctctgtaggt caaaaaatat tactaaatga
      481 cctttgtgaa aaatttgaag taagcagaac accagttgta caagcagtaa aaatgctaaa
      541 taaagatgga gtgcttacta ttatgacaaa tggtaaagtg tacgtaccag agtatgaata
      601 tgatatggta aaacaagttt gtgaaacgag aacactgatt gaaacatatg ctttagaaaa
      661 aatgatgcaa gaagaggaag aggtttttca acaaaagcta gatgtaataa aaagatactc
      721 tgataaatgc gaggagtact atcgtcaaga aaaatctgta gaacttgcac tagcagattt
      781 ggaattacat aaagccattg tagaaggggc aaataatgag attttaaatg atgtatatgt
      841 tggtatacaa ggaagattta tagtagtgaa ctatttaatt cgaccattaa aaaatcgtaa
      901 ttatgaagga acagtacaag accattttga aatattaaaa ttcattgaag acagagatac
      961 aaaaaaagca gtagataaac taagaaatca tattcaagga actattaaaa gattctgtga
     1021 agatgaataa taagttttga agttactata tacttatcta ttaagttgct aaatgtagtc
     1081 taagtataat ctaattatag tttaaacata atctaaatat aatctaaata tagtgtaaat
     1141 aagtctaagt atgaattaaa tatagattaa gtatg
//
LOCUS       DACTGW010000392         1145 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.401, whole genome shotgun sequence.
ACCESSION   DACTGW010000392 DACTGW010000000
VERSION     DACTGW010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.401"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(27..716)
                     /locus_tag="I9573_003647"
     CDS             complement(27..716)
                     /locus_tag="I9573_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAT4798229.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(709..1080)
                     /locus_tag="I9573_003648"
     CDS             complement(709..1080)
                     /locus_tag="I9573_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAT4798230.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
BASE COUNT          410 a          195 c          131 g          409 t
ORIGIN      
        1 caaggtactc gtcttaattt tattttttat ataaaattca ttataattgg aattagtatt
       61 actgtaattg taccagaaac tccaatagaa acaccactca tagctccttc tatttctcca
      121 agttcaaaag cttttgtagt tcctaaagca tgtgcagatg ttccaagagc aattccttta
      181 gcaactggat gatttatatt ccccagtttg aatactgtag aagcaaccat accaccaaaa
      241 atacctgtaa aaattatggc tacaactgtt attgattcta taccatttaa agaatttgct
      301 aaagatattc ccattggtgt tgttatagat tttggaatta gtgaatttat tatacttaca
      361 tctgaattag ctattttgcc aattatcaat acagatacaa aagaaatcac tataccacaa
      421 cctattccta caataatctc aaacatatgc tttttaaata attcaaattg tttataaagt
      481 ggaactgcaa gtactactgt gacaggacct aaaaagaaat ttagactatc agctcctatc
      541 ttgtaattct cataagggat tttagatatg cttaaaaaca atattattcc tacgattgca
      601 attaataaag ggttacatat aggattactt gtttttcgtt gaatgtatct tcctaaattg
      661 aaaaaaacta ttgttactat tataccaaat actggtgtct gtaatacatt atacattttt
      721 ttgtccttct tccttctttt ttatcatttt ctccacaata attccagtag tatacattac
      781 tattagtgta cttaaacata tgactaataa aagcactaat acattttccc ttattaggtc
      841 taatgacttt ataagtgata cacctgctgg aataaaaaat atagccatat tatcgaggaa
      901 aaatccactt acattttcta tactactcaa gtctaaaact ttaaattgta gtagtagaaa
      961 taataatagc ataccaacta tactcccagg aataagtata attccttgta taaaggaact
     1021 tatatactct cctattaccc agatagataa aattattact aattgattga aaactttcat
     1081 tttccacctc caaaaattaa agagattgct tcaaatcata tatttgtagt tttatcaaca
     1141 agcaa
//
LOCUS       DACTGW010000393         1142 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.402, whole genome shotgun sequence.
ACCESSION   DACTGW010000393 DACTGW010000000
VERSION     DACTGW010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.402"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            1..1059
                     /gene="mltG"
                     /locus_tag="I9573_003649"
     CDS             1..1059
                     /gene="mltG"
                     /locus_tag="I9573_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAT4798231.1"
                     /translation="MKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIIDVPSGASV
                     GKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISLLVSGKIY
                     QDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKEDGITSLE
                     GFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVMEMASIIE
                     KEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKIDSPYNSYK
                     NKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKYVKEYKEA
                     RDKQSKDTKATNKENTKK"
BASE COUNT          497 a          124 c          174 g          347 t
ORIGIN      
        1 ttgaaaatag cagtattaat aattgttatt ttgattatct tagcaggtat atttgtgttt
       61 atacaaatag gtccttatga taaaaataac aaaaaagatg taattattga cgttccaagt
      121 ggagcatctg ttggaaaaat atctgacata ttatatgaaa ataaattaat aaagaatgaa
      181 ctattattta aacttttggt aaaggttagc aataaagctc cgtctataaa atcaggaaca
      241 tacttactta atcaatcgta ttcaaacaat gatataataa gtcttttagt ttcaggtaaa
      301 atatatcagg atggtataaa agtgactatt ccagagggag ctacatctaa agaaattata
      361 gcaatgttag taagtaaaaa tttaggagat aaagctactt ttgaaaattt aataaaaaaa
      421 cctcaagaat tttatgataa atttccatat ttgaaggaag atggaataac ctctttagaa
      481 gggttcttgt atccagaaac ttattatttt aattcaaaga aacaatcaga agaagatatt
      541 ttaagcgaaa tgttaaaagt atttgacagt aaatacacgg acaagtttaa aaagaaacaa
      601 aaagaattaa atatgactct tcaagaagta atggaaatgg catcaataat tgagaaagaa
      661 gctgttcttg acaaagacag acctataata gcaagtgtgt tttataatag acttaaggta
      721 ggtatgcctc ttcaatcaga tgctacaata caatatatat ttgaagagag aaagaaaatt
      781 gtaacatatg atgatttaaa aattgattca ccatataata gttataaaaa taagggatta
      841 cctcctacac caatatcaaa tcctggtata aaatctattg aagcagcatt atatccagag
      901 aaaacagact atttatattt tgtagctaag attgatggtg gaaataatta tagtacaaac
      961 tatcaagacc atttaaaata cgttaaagaa tataaagaag caagggataa acaatcaaaa
     1021 gatacaaaag caacaaataa agaaaataca aaaaaataaa ccatgattat aaaaagtcta
     1081 acagggtaag aataatgttg ggctttttta ttgaaaattg ttattcttag atttataata
     1141 aa
//
LOCUS       DACTGW010000394         1091 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.403, whole genome shotgun sequence.
ACCESSION   DACTGW010000394 DACTGW010000000
VERSION     DACTGW010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.403"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            157..909
                     /gene="cwlD"
                     /locus_tag="I9573_003650"
     CDS             157..909
                     /gene="cwlD"
                     /locus_tag="I9573_003650"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HAT4798232.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          484 a          109 c          167 g          331 t
ORIGIN      
        1 ataaactaag tataatttat tgtatagtat tgaatgttag ataaacatag tgaattaaat
       61 aggggcagaa atataatatc attacataat aatatattaa taccaataaa taaagatata
      121 tcaatattaa aaaaatttaa atgtttgtac taatatatgg aatattatag tgtttttata
      181 agtatacata tacagggtga tgttgtgaga aagtacataa aacatataat ttttagtttt
      241 gcaatgatat gtctagtagt agtatccata tttgaaatta aaaatatttc tgaagatgtt
      301 atcaagtata tgccagtaac aaacaaaact ataattttag atgctggtca tggaggcatt
      361 gatccaggtg cattaaataa ggataagagt acatctgaaa aggatattaa cctagcaata
      421 acacttaagc ttagagagct tatagaatca agtggtggcc ttgtaatatt aactcgagaa
      481 gatgacagta gcctttataa agaagaaaat aataaaacta caagacaaaa atataatgag
      541 aatttgaaaa atagaaaaga aataatatct aattctaatg ccaatatgtt tgtctctata
      601 catcttaatg catttgaaca atcaaaatac tatggagctc aaacttttta tcctaaagac
      661 aaacaagaca gtaaagaatt atcgaagtgt attcaagaag aacttaaaag agtagtagat
      721 aagacaaaca acagagaagt caaaccaagg gatgatatat atcttttgaa agaaaataat
      781 attccatcag tactgataga atgtggtttt ttatcaaatg aaaaagagtg taaactctta
      841 actgatgaaa catatcaaga aaaaatagca tgggcaatct acataggaat acaaaaatat
      901 ttaagttgaa ataagtataa aaataggtct aaatatgtaa atagcttgta ttaaagcagt
      961 taaaatgcat taatataggc tatttttatt ttgacaaaaa aatatttaaa ataaaagtta
     1021 aaaagttgtt gacttagaat aatatagatg atattatata tgagtgccca aaaggagcac
     1081 caaaataaga c
//
LOCUS       DACTGW010000395         1034 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.404, whole genome shotgun sequence.
ACCESSION   DACTGW010000395 DACTGW010000000
VERSION     DACTGW010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.404"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(149..859)
                     /locus_tag="I9573_003651"
     CDS             complement(149..859)
                     /locus_tag="I9573_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4798233.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELEKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVHIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT          358 a          139 c          120 g          417 t
ORIGIN      
        1 aactaagctt caaaaaatta aattttgaaa gattgaaatt ctagaaatta agcttcaaaa
       61 aattaaattt gaaaaaacaa aactctaaaa actgagcttt aaaaaattaa gtttcaaaaa
      121 taaaattcta gagattgaac ttcaaaaatt aaggttctaa catatacctt gtaaattttc
      181 ctacaacctc ttgatatttt ttacggctta taggaatttt ttcattctga actttggcat
      241 aagtttgagt tatactatct acatggttta gattgacaat atacctttga tgcgtacgta
      301 taaaattaga gggaagaaca gtttccatat catttaattt tccgtagtat tctaatgttt
      361 tatcaaaggt aactacagtt aattttctta gctcgctttt aaagtagagg atatcttttg
      421 tattaatttt acaaagatta gttccaatat gaactgtaat aaagttttct ttccattgtt
      481 ctaatttttt tagtgcttct tttaatactt tacatatttt ttcagtttta taaggtttta
      541 agatgtagtt caaagctcct acatcatacc catgaaatac atagtcagaa aaacctgtga
      601 caaagatgat tatagtatct tttgaatgtt ttcgtatagt ttttgctatg tcaacgccat
      661 tacttttttc aagttcaata tctaaaaaaa taatatctat gtaataattt tcgtgttcta
      721 gattgtatac tagactttct ccatttgaat attcttttat ttggtagggg tagccccaca
      781 aatccatttc tcttgtaatg attttattca gcatgtttct ttgttgtttt tcatcatcac
      841 aaatacaaat cgaagtcaat tttttatctc ctttcaagtt ttatatttat atagttaagt
      901 atataatata ttttgctacc tagatatata aaatgaaata acaattatat ccagatagca
      961 aaataaaatt ttttatatta gtatacttac acttaaaact attctattaa acttattctg
     1021 aagtgctaat gatt
//
LOCUS       DACTGW010000396         1029 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.405, whole genome shotgun sequence.
ACCESSION   DACTGW010000396 DACTGW010000000
VERSION     DACTGW010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.405"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            183..>1029
                     /locus_tag="I9573_003652"
     CDS             183..>1029
                     /locus_tag="I9573_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAT4798234.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQI"
BASE COUNT          409 a           89 c          182 g          349 t
ORIGIN      
        1 accatctgac ttttttgatt tcattgaata aaaaatgtat ttaattattc taaataatta
       61 ggctatataa atcaactaaa agtttttatt tatcgtattg aattagttat aatattttag
      121 tatagtaaaa aatagtgcta ttttacatat ttaaagagtt taagtagaag agaggtgcca
      181 tattggatta cttaaagtat tttgaaaaag ctgtaatgta tattgagaat aatttacatg
      241 aaaatataac tgtaaatgat gtggcaaaag aaactggata ttcttattat catttaacac
      301 gcttatttaa gtctatgttt ggtgagagtg taggaagtta tataaaaaaa agacgattag
      361 taagtagtac aaaagaactt ttatatagtg ataaaaaagt gatagatata gctattggta
      421 gtggatttga atcttcggaa gcatttagca gagcatttaa atctatatac aaggttagcc
      481 ctatagaata tagaaaaaat agaattgatg tatttgttgg taaaaagaaa aaattagagt
      541 tagattttat gagacatctt gttggaaata taacgattaa accagttata aaagaaatag
      601 aaaaaataaa ggtaataggt ataaaggata aagtaatttt agaaaataat tcattacctg
      661 acttgtggga gaaattcaga aaagtacatc atattgtccc aaatactttg ccatcaaaga
      721 gagtttttgg aatctgtgaa gcaatctctg aaatacacct agttagtgaa agtatggaat
      781 ttaatgagat aataggactt gaagttaata gttatgatgt gattccaaat tcatttgttt
      841 caaaaactat taaggaagga aagtatgcta tatttactca tactggtagc ttagataact
      901 tagacaaaac ttatgaatat atatggggta cttggttttt aagttcaaaa gaagaactgg
      961 atataagaga tgattttgaa gtatatgatg aacgattttt aggtcctagt aatgtgaact
     1021 cacaaattg
//
LOCUS       DACTGW010000397         1008 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.406, whole genome shotgun sequence.
ACCESSION   DACTGW010000397 DACTGW010000000
VERSION     DACTGW010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.406"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            189..701
                     /locus_tag="I9573_003653"
     CDS             189..701
                     /locus_tag="I9573_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798235.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEDYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKAKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT          460 a           77 c          185 g          286 t
ORIGIN      
        1 aatctatcaa tatagataaa atcttattat ttaacttcaa ctattcctta aaaaagcaac
       61 ttttaaggaa tagttgaaac aaaaagtttg gttacataat agtaatttta gttacaagaa
      121 tgaaactgta aaacttagga ttttctttat ataatttcat tgagaacatt taatttagga
      181 ggtaaggtat gaaagtaaaa aaatttaaaa gtggtatagt aatggcatta acagtaatgg
      241 caattggagc atcattgaca aatgtatcag ctatggaatt aaacaagagt aaagagtttg
      301 aaactaaata caatgaaatg aagagggatt ttcaagagga ttatggaaaa gaagacaaat
      361 ttaatataac attggatgat gaatttacaa aagaagaagt aaatggagct aaagggatta
      421 taaagacaac taataaaatg aataatgagg ttgtagtatt aaattatttt gatgaaatga
      481 aatctgggat tgaatttgag ttgaaggcaa aagcagaaaa aaatgcaatt ttgagagatg
      541 aatttcaaca aggttatgga aaggaagata aattcaaagt tgtgttagat gatgaattta
      601 caaaagaaga ggtaaatgga gctaagggca ttataaagac tacagataaa gagacaggtg
      661 aggttatcgt atataattac tttgatgagc ttgaaaaata atgcttaaag attactttga
      721 ttgaatgaaa aggtaattgt gttgagaata ttaaatgact gaataaaaac agttgtatca
      781 aaaacaaaca ataatagaat gtaagaatga ttgtgtcgaa aatattaaat gactgaataa
      841 aaaatagttg cgtcaaaaac aaacaataat agaatgtaag aatgattgta tcaagaatat
      901 aaaatgattc aacaaaaaac gattgtgtta aaaacaaaca acaatagaat gtaagaatga
      961 ttgtatcaag aatataaaat gactgaataa aaacgattgc gtcaaaaa
//
LOCUS       DACTGW010000398          990 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.407, whole genome shotgun sequence.
ACCESSION   DACTGW010000398 DACTGW010000000
VERSION     DACTGW010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.407"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            134..922
                     /locus_tag="I9573_003654"
     CDS             134..922
                     /locus_tag="I9573_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAT4798236.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
BASE COUNT          389 a          124 c          177 g          300 t
ORIGIN      
        1 aaaatacacg agtgttttat aaataaaatg taataaataa ttaattaaca aatataaatt
       61 ttaaaataag ttagatattt agaaaaatcg aaatttgtag gatttaaagg acatattcaa
      121 ggaggagtta ataatggaaa aattacaagg aaaaattgca gtagttactg cagcaacaaa
      181 aggtattgga ttagcatcag cagagatatt agcaaaaaat ggagcaactg tgtacttagc
      241 agctcgttca gaagaattag ctaatgaagt tataaataaa ataagtgcag aaggtggttg
      301 tgctaagttt gtttacttta atgctcgtga agaagagacc tttacctcaa tgatagaaga
      361 ggtagttaaa aaagaaggta agatagatat acttgtaaac aattttggtt caacaaaccc
      421 ttctcttgac aaagaccttg taactgggga tacagataat ttctttgaca cagtaaatac
      481 taatttaaaa agtgtgtatt taccatgtaa agcagcaatt cctcatatga taaagaatgg
      541 aaaaggaagt atagtaaata tatcaagtat aggttcagta ttacctgatt tatctagaat
      601 agcttactgt gtatcaaaag cagcaatcaa ctcattaact caaaacatag ctacacaata
      661 tgctaaagac aatgttagat gtaatgctgt acttccaggg cttattgcaa ctaaagcagc
      721 attagacaat atgtctccag agttcataaa agaattttta aagcatgttc ctttaaatcg
      781 tataggggaa ccagatgata tagcaaaagc tgttctattc tatgctagtg atgactcatc
      841 atttataaca ggggatttac ttgaagttgc aggaggattt ggtttaccta ctccacaatt
      901 tgcagataat atattaggat aattaaatta ctaatatgca atgaaagctt atgcattagt
      961 tatagtagaa atgatatttt tataaaggcc
//
LOCUS       DACTGW010000399          980 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.408, whole genome shotgun sequence.
ACCESSION   DACTGW010000399 DACTGW010000000
VERSION     DACTGW010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.408"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..831
                     /locus_tag="I9573_003655"
     CDS             <1..831
                     /locus_tag="I9573_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HAT4798237.1"
                     /translation="NVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNSEVDGYSDFA
                     NTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEFG
                     AVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDFG
                     IRGQINNFDISNLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPES
                     KSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWML
                     "
BASE COUNT          410 a          106 c          146 g          318 t
ORIGIN      
        1 aacgtacttc aaattgacgc agaattaaaa aatatcatag ttagaaatcc tggagaagca
       61 tctccattta gattgggtga tgctatagta aaggtaaata attctgaagt tgatggatat
      121 agtgactttg ccaatacctt aaatgcttta ccagaagaaa aacaagtatc tattttatta
      181 aatagaggtg gacaacttat aacaatgaaa actaccaaac acatattaga aaaaattagt
      241 tttaacaatt tactttctgg atttgctact ttaacgtata tcaatccaga tacatcagag
      301 tttggagctg taggtcatcc aataagtgtt ggtaatgcaa gaaaaatacc tattaaaaca
      361 ggttcaattt ctacaactaa gcaccttaat atagaaaaat cttataaagg tagtgttgga
      421 tgtataaatg caaaacgaga aaatattatt ggtagcttta cagaaaatac tgattttgga
      481 ataagaggtc aaattaataa ctttgatatt tccaatttaa aaaaatataa agtagcatct
      541 ttagatgaag ttaaattagg aaaagctcaa ataattttac aagataactt taataagtgt
      601 aaaaaatata atattgaaat tatagatata gaaaaccaaa aacatccaga aagtaaaagt
      661 tttaaaatta aaattacaga taaaaagctt ttagctcaaa ctggtggtat tgtacaaggt
      721 atgagtggta ctccaatagt tcaagataat aaaataatcg gagcagtgtc acatgcaatt
      781 gaaaatgacc cagctgtagg ttatggtgta tttattaaat ggatgttgta aataatataa
      841 ataaataagt tttaagatgt tattaagtta ttaataatat atttggtcta aaggtacttt
      901 tatttaacct acagaccaaa tatattagat attttacata gtataaaata taaattttta
      961 ttaaaattat attaatatta
//
LOCUS       DACTGW010000400          966 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.409, whole genome shotgun sequence.
ACCESSION   DACTGW010000400 DACTGW010000000
VERSION     DACTGW010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.409"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
BASE COUNT          373 a          122 c           90 g          381 t
ORIGIN      
        1 ggtaaaactg ggaatattta accttaagga gttaagttat agaaactagt actaaaaaat
       61 tttataaatt atgttaattg actaatagaa tgcgactttt tatttttact aaatttttta
      121 gggggatgga taatatgttt attggagaaa tagacaaatg tacacatata ctaactgctt
      181 acatcagtag ttcatatgat tattgtaatt ttttagatac acagttagat gattttatat
      241 cagaatatgg agaaacggta gttgaaacct gcttacatca agtcttatta ttagtaagta
      301 gatacaatta aaattaaatt tttctagaaa aaccttttta gatttatcta ctctaataaa
      361 tatataattt tttgtacttt ataagtaact ataacttctg ctaaagtaaa gatgtattgg
      421 gagtacatct ttactttccc attatagaag ttaaaagcat acaccataaa atcaatcatc
      481 caatacaaat tatatatttc ctatgtctaa cttaatttac aagaatcatt ctatatgcat
      541 attctgcaat ccactaaaca tgctctaaac ctttactatt ttatacattc tctataaatc
      601 atacaaatca tatataaaat ccccctaata tgtttaaacc acctataaaa taacaccaat
      661 atttagaata ttcaatatca actgcataac tggtcaataa aaacgtataa ctagacaaat
      721 ttcaatattt atataaataa tacaagaaaa acaagcattt tatttatata aattttttat
      781 tttttgttta tattaaaaaa ctatattgat taatttattt tatttagtaa ttatgaaaat
      841 tcgtcttaga atatattcta tatttgtaga ttagctttaa tatttttaat ttcacaaaaa
      901 ttaaactccc aatattttta tccactttta tctcattaat taattatatt atttttatgc
      961 ttttta
//
LOCUS       DACTGW010000401          959 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.410, whole genome shotgun sequence.
ACCESSION   DACTGW010000401 DACTGW010000000
VERSION     DACTGW010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.410"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            269..928
                     /locus_tag="I9573_003656"
     CDS             269..928
                     /locus_tag="I9573_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAT4798238.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISIFVLIHIILKIKIRKI
                     R"
BASE COUNT          355 a           71 c          112 g          421 t
ORIGIN      
        1 aaaatatatt ttactatttt taattattac aatataaacc tgtcttttgt tattataggt
       61 aaactaaaag taagaaatga tttttatatt tttaaacttt attattggta acttatctgt
      121 aaatatttta aaatgtacta tagaaagttg ttaaaaatat taaataatta tattaaattt
      181 aagtgtgttt tttatgctat aattagtatg tattttttat gatatatttt ataaaaaata
      241 taatttaatc atttttgagg atattattat gttagatgca tttataacaa ttgtttcttc
      301 tattatagaa atatggtcat gtaaaaaagt ttttgattat actagtcagt caaagaccga
      361 cttaaaaaaa ctaaatatgt attttctctt ttctgttttt cttattattt cttctttata
      421 tataaatata ggagctaata gtagaatctt gatatgtata tttgtaatgt tcgttttcta
      481 taagtctaac tataaggtta atatatataa atgtctggta atctcatttt tttattggtt
      541 aatattaata ggtataaatg gattaagtat gttattagta gcaacagtaa gcaatttgga
      601 taatatgaat gttttactta gtggcagttt atatagatat gaagcaatat tcttgggaaa
      661 gataatgtta ggtatattac tttatgttta taaggcttct tttaaaaatt ttaaaataag
      721 taaaaaagag atatacatat gtattcctat gatagcaaat ttattgtcat tttttgtact
      781 ttatgattat attattaatc ttgtaaaaat ctctcctgaa agaagaactg cttttttagt
      841 aatagtttta ctattaatat tatctaacat ttcaatcttt gtattaatac atataatact
      901 taagattaaa attaggaaaa taagataaat tttaatacga ttcatttaaa ttaaaagtt
//
LOCUS       DACTGW010000402          905 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.411, whole genome shotgun sequence.
ACCESSION   DACTGW010000402 DACTGW010000000
VERSION     DACTGW010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.411"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            483..>905
                     /locus_tag="I9573_003657"
     CDS             483..>905
                     /locus_tag="I9573_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798239.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFC
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPR"
BASE COUNT          366 a           92 c          140 g          307 t
ORIGIN      
        1 aaatcataac tttattttta tatttgttcc attttcataa aaattattat ccctttaaga
       61 aaatttctta atcctcttga aatatttcct ttttagtcta aagttaaatt tctttcttca
      121 aaatgaatat ttaaatgaac taaaatttaa gtacattttc taatcttatg agtctatttt
      181 tcatagataa ttgccctata atagaagaat ctatgttggg gctatgtggt gacaataaca
      241 gcattgtcag tgattaatta atgctttata aatgattaat tagtgtttta tgttattttt
      301 ggacttaccc tctatacata gattaattta tttaacttta gatatagaga gaatgtcaag
      361 tacatacaca gacattagag tttaagaaag tgtttatgga gcaagaactt ttaaaagaag
      421 tagtgactta gtttaatttc aagaaatgat aaatacaaaa gaggaaaaag agaggtgttg
      481 aaatgataga aaaagtagaa gaaaaatcta agattattaa aacatcaaaa gaatataagc
      541 gaactataat tgaagaaaaa gataaagaat ggaccaaaaa attaaatgat atacttagta
      601 gaagtagttt ttgtaatgat gaattagata aaatcaaggc gttaattcca aaagagattt
      661 tgactaatga aaatgttgga gaagttgtga ctgaagatgg atatgctaag cccgagtatg
      721 atgaggtatt taaatcattg ttcactttaa ataatgaata tgatttactt gtaaatttta
      781 taaatgatat tctaaaagat gctccatatg ctaacaggaa tatcaaaccg ttcactcaaa
      841 taaagagaat aattagagtt gaaactgatc caacaattaa ttacattgga gagaaaagac
      901 ctaga
//
LOCUS       DACTGW010000403          895 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.412, whole genome shotgun sequence.
ACCESSION   DACTGW010000403 DACTGW010000000
VERSION     DACTGW010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.412"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>895
                     /locus_tag="I9573_003658"
     CDS             <1..>895
                     /locus_tag="I9573_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT4798240.1"
                     /translation="AIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGN
                     LKYKLEVIGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTS
                     ILSYVDMLKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTD
                     IRELLIQSIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMP
                     NTRVYIDMFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQ
                     NIIELENAHMEIIIDADLFKVM"
BASE COUNT          383 a           92 c          133 g          287 t
ORIGIN      
        1 gcaatagcta tagtaatttt atttacactt gtaaacacag tttttcattt ttatatatac
       61 aactttttat ctgttaagtt taattacata aataacatat caaaaaatat aaaaaatata
      121 gaaaatggca atttaaagta taaattagaa gttataggag atgatgaaat atctacttta
      181 gccaaaagta taaataatat aacaggtggt cttgaaactg ctgtagatgc taatataaaa
      241 aatgaaagga tgaaggcaga gcttatcaca aacgtatctc atgacttaaa aactccactt
      301 acatctatat taagctatgt tgatatgtta aaaaataatg actttgacag agagactata
      361 aatgactata taaatatttt agataaaaaa tcacaaagat taaaattatt aatagatgac
      421 atatttgaag catctaagct aagtagtgga gatgtagaat ttaatataac aaaaaccgat
      481 ataagagaac ttttaattca aagtattgta gagtttgaag acaaaattca aaatagcagt
      541 ttagatttta taatagaaac acctgaatat gctgttttta ccatgataga tggtaaaaaa
      601 acttggagag tttttgagaa tctaatttgc aatatactaa aatactctat gcctaacact
      661 agggtctata tagatatgtt tataaaggaa gaaaatataa tattaacgtt taaaaatatt
      721 tcaaatgata aactaaatct aaagccagaa gagttaatag aaagatttag aaggggtgat
      781 atatctagaa aaacagatgg ctctggtctt ggtttatcaa ttgctcaaaa tataattgaa
      841 ttagaaaatg cacacatgga aataattata gatgctgatt tatttaaggt aatgc
//
LOCUS       DACTGW010000404          832 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.413, whole genome shotgun sequence.
ACCESSION   DACTGW010000404 DACTGW010000000
VERSION     DACTGW010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.413"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(114..767)
                     /locus_tag="I9573_003659"
     CDS             complement(114..767)
                     /locus_tag="I9573_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAT4798241.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKFMRENDLENVERVLREHWKTIPIAEMKKEKLL"
BASE COUNT          253 a          142 c           93 g          344 t
ORIGIN      
        1 tctatcgtag tgccttagag tttttatttg tatttaaagt taaatttatt cttgtttttc
       61 ctttaatatt taatagtaaa ataacaaaat tataaaaaaa ataaagagaa actttataaa
      121 agcttctctt ttttcatctc tgctataggt atcgttttcc aatgttctct caaaactctt
      181 tcaacatttt ctaaatcatt ctccctcata aattttacta cttcaatatg ttgattattg
      241 ctgtatgtca acattttata taactcatct tcattatcac tagtatatga ctttgacata
      301 aaattatatc ttaaagagtt caatagattt actaacacct gattatctga tttagttatg
      361 tatatatcgt gaaattcatt acttaatttt aaatagctat tgtaattttt atactttata
      421 gagaccgcca tcatttctgt taattcttcc atttttacta ggtctgattc agttaaattt
      481 tgtaatgctg aagaaccggc taacgcatcc aatactccaa ttacggaata ggtgtctaat
      541 ttctcctttt cctgaaattc ttttactaca aatcccttat taggtatttt ttctagtaaa
      601 ttttcacctg ctaacctagt taatgcttct ctagctggtg ttctactaac tccgatagat
      661 ttacaaatct cagattcagt aattctctga ttagcttcat actctccatt ttggattttc
      721 tttgatatat agtcatatac ttgagttgta agtggatcta ccttcataag acgttcctcc
      781 atattaagat tataaaagtt aaatctccct acaactttgg tagagagttc ag
//
LOCUS       DACTGW010000405          805 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.414, whole genome shotgun sequence.
ACCESSION   DACTGW010000405 DACTGW010000000
VERSION     DACTGW010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.414"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(131..700)
                     /locus_tag="I9573_003660"
     CDS             complement(131..700)
                     /locus_tag="I9573_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HAT4798242.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKSIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
BASE COUNT          268 a          157 c           67 g          313 t
ORIGIN      
        1 tatattttta tcatttcaac aatgaaaaag ggatttatct ctccattttg aaacaacccc
       61 tttttctatt aattactttc ttaaaaaata taatacgata tataagataa atatctaatt
      121 gtacctattt ctaattatat ctattagaat acaacacctt tgccttttca gtaaactccc
      181 taacaaaatc tagtttacta tctgtataag catcacgatt atgctcatac ttcttccaaa
      241 ggtccaactt aagtttttca tactcctttg caacatctgg gtggtcaatt aagtaatctc
      301 taaaaaacaa ctcatcatta tcacctacat accttatatg caaatgaaaa acgcgttctt
      361 caaacccatc tactgtatac cctttattaa gtgaaagcct actactgctc tcagacatac
      421 aaatatatcc attcgactca atcaaatctt taatatcttt catattataa tcaggaatta
      481 tctcaatcag tatgtcaaca atggacttgg cataaatcgt actcacagaa gtacttccaa
      541 tatgacttat tctaacaatt ttttgcattg gcaatattcc agttagtcta tattgttctt
      601 ctttaaacca atttttccaa cattctttgt gagtagtcaa aaatatagga aacaagttcc
      661 ataactcctc taaagtcata tcttccaact tctttttcat tagttacctc cttttaaatt
      721 tgtactttat attttttctt tttcaaattt acttagttaa tatttaactt tattttttat
      781 caaatcattt ttctaattat gtaaa
//
LOCUS       DACTGW010000406          804 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.415, whole genome shotgun sequence.
ACCESSION   DACTGW010000406 DACTGW010000000
VERSION     DACTGW010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.415"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..604
                     /locus_tag="I9573_003661"
     CDS             <1..604
                     /locus_tag="I9573_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAT4798243.1"
                     /translation="LILISSLCVIIYSYKITSLYIFKNFKIGILLVFYFLFSQIIFGL
                     IISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSFLQSILFDRFGKKIGIVMVG
                     TCWSLWHLPLQFTLYSPQTPIIGAIAHLIYGIGLSIFLGYVYMKTKNIWFCSIMHVLI
                     NSLGLIFNDSEIIINYHSIIQRLIFILLFYTPFLFTKEYK"
BASE COUNT          287 a           75 c           95 g          347 t
ORIGIN      
        1 gttaatttta ataagtagtt tatgtgttat tatttattct tataaaataa caagtttata
       61 tatttttaaa aattttaaga taggtatttt gctagttttt tactttttat tttctcaaat
      121 tatttttgga ttaataataa gtgataatca atttgattat aagaatttct tgaattatat
      181 tcttcttcct ataatatcat tagtatatat ttatcctttt ttatgtgaag agtatggttg
      241 gagatctttt ttacaaagta ttttatttga tagatttggc aaaaaaatag gtattgtaat
      301 ggtaggcact tgttggagtt tatggcactt acctttacaa tttacactat atagtcctca
      361 aactccaata attggagcta tagcccattt gatatatggt attggacttt ctatattttt
      421 aggttatgtt tatatgaaaa ctaagaacat atggttttgc tctataatgc atgttctaat
      481 taatagctta ggattaattt tcaatgattc tgaaataatt attaattacc atagtattat
      541 acaaagattg atatttatac tattatttta tactcctttt ctatttacaa aagaatataa
      601 gtaagaatta caattaattt atataaaata aatttttaag taaatatttt tatagagaga
      661 ttgttatatt acatatcaga aaaatattaa acgaactaaa tcaataaaaa agttggtggc
      721 taacaattta taattgttag cacaatataa tcttatgtat tgaaatctag aatatcctca
      781 tttttactta gcataaatac tatt
//
LOCUS       DACTGW010000407          800 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.416, whole genome shotgun sequence.
ACCESSION   DACTGW010000407 DACTGW010000000
VERSION     DACTGW010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.416"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            94..660
                     /locus_tag="I9573_003662"
     CDS             94..660
                     /locus_tag="I9573_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HAT4798244.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT          309 a           66 c          149 g          276 t
ORIGIN      
        1 atttaataaa tataaattaa attatatgtg tatatattgt actttaatag tgatataata
       61 ataaaaaaat ttttaaagag ggtgatatta catatgagaa aacttgaaga gatattagaa
      121 tataataaat catttgtaga gaacaaagaa tatgaacaat atgtgacatc aaaacatcca
      181 aataaaaaaa tagtagtctt atcttgtatg gatacaagat taactgagct tttgcctaag
      241 gctatgaatt tgaaaaatgg agatgtgaaa ctaataaaaa atgcaggagc tactataatg
      301 catccttttg gaagtattat gagaagtgtt ttagttgcaa tttacgaatt tgatgtagat
      361 gaggttatgg ttgtagggca tcatggatgt ggtatgtgta atgttgatac agataagtta
      421 cttggtaaaa ttttagatag aggaatatct aatgatgtaa tacttacact tcgtagtgct
      481 ggaattgata caaaacaatg gttacatgga ttcgattctg cagaagaatc tgtaaaagat
      541 agtattgatt taataaaagg gcatccttta attccagatg gtattattgt acatggtcta
      601 attatatcac ctgaaactgg gaagttagac gttgttgtaa atggatatga agataagtag
      661 agtattgtaa tgttatataa tcaagatact gttgaatatg atgttgtttc aaaatttatt
      721 ttgggacaac ttttttattt gaaaatatat gaaaaggttt attttaagct ttacaataaa
      781 aaatataagt agctgactaa
//
LOCUS       DACTGW010000408          787 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.417, whole genome shotgun sequence.
ACCESSION   DACTGW010000408 DACTGW010000000
VERSION     DACTGW010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.417"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            47..>787
                     /locus_tag="I9573_003663"
     CDS             47..>787
                     /locus_tag="I9573_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAT4798245.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHIIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNVIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNH"
BASE COUNT          329 a           91 c           95 g          272 t
ORIGIN      
        1 ccatcataaa tttatataaa catgtaatga atggagtgat tttttattga gaaataaaat
       61 ttttttatca atacttatta taatatcact tttaatcggc tgtgacaaaa aatctctact
      121 atctatacat ataattgatg ttggacaagg tgatagtatt ttagttcaaa cacccacaaa
      181 taagaatata ttaattgatg gaggtgatga agatagcgaa aatataatta ttagttatct
      241 tagacaaaaa agaataaaaa ctattgatat tataatagca actcatccag attcagacca
      301 cataggaagt cttgataatg ttataaaaaa atttaatgta aactccatat atatgcctga
      361 acaatcaact gatagtgagg cttatcaaaa tctaataaat tcttgtacgg ataaaaactt
      421 atctattcag catctctata aaaatgatgt tttaaacata gataacaata taaatatata
      481 tgtattaagt ccatcatata tacaagaaga atctaattta aattctatag ttttcaaatt
      541 aacttttaac gataattctt ttttatttat gggtgatgct gaagaagaaa atgaaaaaga
      601 aattttacat tcttttaaat taaataatat taattttata aaaataggtc atcatggaag
      661 taattcttct tcatctttag aatttataaa aaaaattagt ccagatatag ctgcaatatc
      721 atgtggatat aaaaatcaat atggtcatcc acatagggag gttattaata atctaaaaca
      781 aaaccat
//
LOCUS       DACTGW010000409          787 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.418, whole genome shotgun sequence.
ACCESSION   DACTGW010000409 DACTGW010000000
VERSION     DACTGW010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.418"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..576)
                     /locus_tag="I9573_003664"
     CDS             complement(<1..576)
                     /locus_tag="I9573_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAT4798246.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATV"
BASE COUNT          266 a          128 c           79 g          314 t
ORIGIN      
        1 tacagtagct atatctctaa gcttttctat aaagtctaaa tcatttataa ttatttcagt
       61 tggatggatt tttgcaattt cttcaattac cttatcttca tttaaacatg ttgcattagt
      121 ttctcctgta cttatatcaa cataagttag acctatatta gtaccatcct tatacaaaga
      181 taataaataa ttattctttt tattttcaag taaatttcca tctaaaactg taccaggagt
      241 gatgactctt ataacctctc ttctaacaat tccttttgct gtagatgggt cttccatttg
      301 ttcacctatt gctactttat atccattttc aactaatttt gatatatatg aattggctga
      361 gtggaaagga acaccacaca taggagcacg ttcttctaaa ccacaagctt ttcctgtcaa
      421 tgctatttca agagcttttg aagctactaa tgcatcttca aaaaacattt cataaaaatc
      481 tccaagtcta aaaaacagta tacagtcttt atatctattt tttacttcaa gatactgttt
      541 catcatagga gttaatttat tcatatctat ttgcattatc aaacctcctt cttttctaat
      601 taaatattat atcatagtag atgattatta ttaacacaat atataaaatt ttgacatata
      661 actaaaaaca atccaaaatg tatactcaca ttttggattg ctaagtttaa tgcttattaa
      721 ataaaaatat atatattttt acaataaaga tattatttct tttttaagtt taatgatatt
      781 ttcaagg
//
LOCUS       DACTGW010000410          781 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.419, whole genome shotgun sequence.
ACCESSION   DACTGW010000410 DACTGW010000000
VERSION     DACTGW010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.419"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            66..>781
                     /locus_tag="I9573_003665"
     CDS             66..>781
                     /locus_tag="I9573_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HAT4798247.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPG"
BASE COUNT          312 a           89 c          162 g          218 t
ORIGIN      
        1 attaaaaaag tgaaatatca aaataaaacg tcaacaaatt aaaaattata tgggggaata
       61 tcaaaatgtt aaatgaaaca agaaaaaaac aattaactga agtatgccaa gacttaataa
      121 gaaatgctag ttattcaggt caagaagaaa acgtagtaaa agctatagaa gaaaattttg
      181 ctttactagg ttttgatagt tggtcaagag atagatatgg aaatatagta ggatgtataa
      241 aaggaaataa accaggtaaa aaaatattat ttgacgggca tatagataca gttccagttc
      301 cagatgcaag taaatggagt gttcctcctt ttgaagcgag aatagtagat gggaaaatat
      361 atggtagagg aacttctgat atgaaggggc aagtaagtgc tatgatggct gctgtttcat
      421 actttgcaga agatactaac aaagactttg aaggtgaatt gtatgtagca ggtgttgttc
      481 atgaagaaat atttgaaggt gtttcagcta gagagataag taaggctgtt caacctgatt
      541 atgttgtaat aggtgaatct tcagaattaa acttaaagat aggtcaaaga ggtagaggag
      601 aaatagtagt agaaactttt ggtaaaccag ctcactctgc taatccagaa aaaggtataa
      661 atgcagttta taaaatggct aatgtaatcc aaaagataca acaattagta cctccaactc
      721 atccagtttt aggtgatgga atattagttt taacagatat aaaatcttct ccatatccag
      781 g
//
LOCUS       DACTGW010000411          760 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.420, whole genome shotgun sequence.
ACCESSION   DACTGW010000411 DACTGW010000000
VERSION     DACTGW010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.420"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>760
                     /locus_tag="I9573_003666"
     rRNA            <1..>760
                     /locus_tag="I9573_003666"
                     /product="16S ribosomal RNA"
BASE COUNT          204 a          168 c          239 g          149 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtactag gtgtcggggg ttaccccctt
      601 cggtgccgca gctaacgcat taagtactcc gcctgggaag tacgctcgca agagtgaaac
      661 tcaaaggaat tgacggggac ccgcacaagt agcggagcat gtggtttaat tcgaagcaac
      721 gcgaagaacc ttacctaagc ttgacatccc aatgacatct
//
LOCUS       DACTGW010000412          752 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.421, whole genome shotgun sequence.
ACCESSION   DACTGW010000412 DACTGW010000000
VERSION     DACTGW010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.421"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>752
                     /gene="tuf"
                     /locus_tag="I9573_003667"
     CDS             <1..>752
                     /gene="tuf"
                     /locus_tag="I9573_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788515.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAT4798248.1"
                     /translation="ELLELVEMEVRDLLTEYDFPGDDTPIVRGSALMALEDPKSEWGD
                     KIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEVEL
                     VGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAH
                     TKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTME
                     VDLINSIVVEEGLRFSIREGGRTVASGVVATI"
BASE COUNT          310 a           94 c          187 g          161 t
ORIGIN      
        1 gagttattag agttagtaga gatggaagta agagatttat taacagaata tgatttccca
       61 ggagatgaca ctccaatagt aagaggttca gcattaatgg cattagaaga tccaaagagc
      121 gagtggggag ataagatagt agaattattc gagcaaatag atgagtatat accagctcca
      181 gagagagata cagataagcc attcttaatg ccagtagagg atgtattctc aatcacagga
      241 agaggaacag ttgcaacagg aagagtggaa agaggagtac taaaagtaca agacgaagta
      301 gaattagtag gattaacaga agcaccaaga aaagtagtag taacaggagt agagatgttc
      361 agaaaattat tagaccaagc acaagcaggg gataatatag gagcattatt aagaggagta
      421 caaagaaacg agatagaaag aggacaagta ctagcaaaga ctggatcagt aaaggcacac
      481 acaaagttta cagcagaagt atatgtactt aaaaaagaag agggtggaag acatacacca
      541 ttctttgatg gatatagacc acaattctat ttcagaacaa cagacgtaac aggagcttgt
      601 aagttaccag aaggaataga gatggtaatg cctggagata acgtaacaat ggaagtagac
      661 ttaataaact caatagttgt agaagaggga ttaagattct caataagaga aggtggaaga
      721 acagtagctt caggagttgt tgctacaata at
//
LOCUS       DACTGW010000413          750 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.422, whole genome shotgun sequence.
ACCESSION   DACTGW010000413 DACTGW010000000
VERSION     DACTGW010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.422"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(158..535)
                     /locus_tag="I9573_003668"
     CDS             complement(158..535)
                     /locus_tag="I9573_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT4798249.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT          277 a          110 c           58 g          305 t
ORIGIN      
        1 aaacaggttt gattttacat caaacttatt gtattaatat taaataacaa gacaaaatat
       61 ttataaaaat tacaaatttt acataaaact ttatatttta ttcatttaac taattattgt
      121 aaaaactatt tattttatga taattaacta caccaaatta ttcatctttt ttaatatctt
      181 taacccattt ttcaacagag ttcaattctt cttcatttac cccctttttg aacaaggatt
      241 tcataaaact ctcaatagaa cttccatgca tattatctaa aaaagttttt gtttcaaaat
      301 gtaaatactc ttcttctcca ataagaactg tataatgtgt atgtctatca atctttcttg
      361 cacttaagaa ttgcttattt actaatcttg ataaaagtgt tagtgttgta gtttgcttcc
      421 aattatatag tttttccata gcttcaatga catcttttga tgctactgta aaatctgaac
      481 tccaaataaa tttcatgact tttaattctg ctggtggtat cttagtaata gtcatatgat
      541 tttacactcc tctcaaactt ctacataatt aaatactata ttaatagact tttcgagtca
      601 aaggttttat ttaaaatatc tcatccaaat ttattaatac aaaaaattta tatttttatt
      661 caaaattaaa cacacaatct attaatataa agataaatat ctattctata agtataacag
      721 attatcaaat taaaattcta atatttgaca
//
LOCUS       DACTGW010000414          746 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.423, whole genome shotgun sequence.
ACCESSION   DACTGW010000414 DACTGW010000000
VERSION     DACTGW010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.423"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            15..105
                     /locus_tag="I9573_003669"
     tRNA            15..105
                     /locus_tag="I9573_003669"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49..51,aa:Ser,seq:gct)
     gene            114..190
                     /locus_tag="I9573_003670"
     tRNA            114..190
                     /locus_tag="I9573_003670"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:148..150,aa:Pro,seq:tgg)
     gene            276..351
                     /locus_tag="I9573_003671"
     tRNA            276..351
                     /locus_tag="I9573_003671"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:309..311,aa:Trp,seq:cca)
     gene            412..488
                     /locus_tag="I9573_003672"
     tRNA            412..488
                     /locus_tag="I9573_003672"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:446..448,aa:Pro,seq:tgg)
     gene            495..571
                     /locus_tag="I9573_003673"
     tRNA            495..571
                     /locus_tag="I9573_003673"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:529..531,aa:Ile,seq:gat)
     gene            575..650
                     /locus_tag="I9573_003674"
     tRNA            575..650
                     /locus_tag="I9573_003674"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:608..610,aa:Phe,seq:gaa)
     gene            658..734
                     /locus_tag="I9573_003675"
     tRNA            658..734
                     /locus_tag="I9573_003675"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Met,seq:cat)
BASE COUNT          175 a          171 c          215 g          185 t
ORIGIN      
        1 aaatgactga atttggagaa gtactcaagt ggctcaagag gatcccctgc taagggatta
       61 ggctgggtaa ccggtgcgag ggttcgaatc cctccttctc cgccattcaa aaacgaggtg
      121 tagcgcagtt tggtagcgca catggtttgg gaccatgggg ccgggggttc gagtcccttc
      181 acctcgacca acttaagctt ttagttcagt tggttagagt gcccaactca tggctagtaa
      241 ttctagggtt tgagtccatg aaggttattc ttcatagggg tgtagctcag ttggtagagc
      301 gtcggtctcc aaaaccgtgc gccgggggtt cgagtccctc cacccctgcc aaataaaaag
      361 gacttgaaaa aagttggatt ttagagtagt ataagatagt aaataagatt tcgaggtgta
      421 gcgcagtttg gtagcgcaca tggtttggga ccatggggcc gggggttcga gtcccttcac
      481 ctcgaccaag aaaagggcct atagctcagg tggttagagc gcacgcctga taagcgtgag
      541 gtcgctggtt cgagtccagc taggcccacc atttgcccag atagctcagt cggtagagca
      601 ggggactgaa aatccccgtg tcggtggttc gattccgcct ctgggcacca ggaatacggc
      661 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      721 tattaccgct accatctaac tatatc
//
LOCUS       DACTGW010000415          733 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.424, whole genome shotgun sequence.
ACCESSION   DACTGW010000415 DACTGW010000000
VERSION     DACTGW010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.424"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..>733)
                     /locus_tag="I9573_003676"
     CDS             complement(<1..>733)
                     /locus_tag="I9573_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAT4798250.1"
                     /translation="DGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKL"
BASE COUNT          277 a          108 c           74 g          274 t
ORIGIN      
        1 atagttttaa atccactaat acaataggaa tactatcatg atttaaagga attgtcgata
       61 ttattatatc tacatctgac aattcactag ctaaaaattg attaaatgat agacattgaa
      121 gtatatttat tttatcatga aaaactttgt ttaattgaac ctctaataac ctacttgaac
      181 cataccctga accacatact aatactacgt ttttacactt aatattattt tgaaaaaatc
      241 tctctattcc agcacctata tgcagggata catatccaat ttcatcttct gttaaagagt
      301 aaatactatt tttaaaaact ttttctatag catttaaagt tatttcaaat gctaaaggat
      361 aattactctt tattgtattt ataaggggat tcaccttatt taaattataa gatttagaat
      421 tgagaattga tttaaagtgc agtactaaat catcaaataa tacactatca tttctaaggt
      481 caaaagtata ttgattgttg attacttcta aaatttcttc aacatactct ataattttag
      541 tatccaccct attggaaacg ttatccaaaa gagaactttt agttataaaa tgattatata
      601 gataaactct gtctgccttt gaaataatta tagtatattt actctcaata taattaaata
      661 tattttcaac agttgtattc gattgaaaat ctgttaaaac aacatctaaa acattatcta
      721 agcagtaacc atc
//
LOCUS       DACTGW010000416          723 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.425, whole genome shotgun sequence.
ACCESSION   DACTGW010000416 DACTGW010000000
VERSION     DACTGW010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.425"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>723
                     /locus_tag="I9573_003677"
     CDS             <1..>723
                     /locus_tag="I9573_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005209661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HAT4798251.1"
                     /translation="KNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGD
                     STTMYPSQNISDEIKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRT
                     VPYISKVSGVPIVDLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEV
                     SLGPEMKSTGEVLGVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAK
                     DMKELGYTFVATEGTAKSLRENG"
BASE COUNT          292 a           75 c          154 g          202 t
ORIGIN      
        1 aaaaatccag tacttataga taaatatcta actggtagag aaatagaagt agatgctata
       61 tgtgataagg aagatatatt gatacctggt ataatggaac atttagaaag agctggagtt
      121 cactctggag atagtacaac aatgtatcca agtcaaaata tatctgatga aataaaagaa
      181 aagatagtag aatacactaa aaaaatagct ttagacctta atgtgcttgg tatggttaat
      241 atacaattta tagaatttca aaatgaatta tatataattg aggttaaccc tagagctagt
      301 agaacagttc catatataag taaagtaagt ggagtaccaa tagttgactt ggctactaag
      361 tgtatgttag gagctaaatt aaaggattta ggatatggta caggagttta caaagagcca
      421 aaattagtat cagtaaaagt accagtattc tcaatgtcta aattatctaa agttgaagta
      481 agtctaggac ctgaaatgaa gtctacagga gaagtcctag gtgtaggaga aaatttagaa
      541 gaagccttat ataaagggtt cttggcagca ggtagacata tgtctgatga aagaggagta
      601 gtacttgcta ctgtaaataa tcatgataaa gatgagttta tagagatagc aaaagatatg
      661 aaagaattag gatatacttt tgttgctaca gaaggaacag cgaagagttt aagagaaaat
      721 gga
//
LOCUS       DACTGW010000417          722 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.426, whole genome shotgun sequence.
ACCESSION   DACTGW010000417 DACTGW010000000
VERSION     DACTGW010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.426"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..444)
                     /locus_tag="I9573_003678"
     CDS             complement(<1..444)
                     /locus_tag="I9573_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4798252.1"
                     /translation="MKYLIEIAKKERLLVFIYLALGIGIELLNSFSANYYQSLIDRFN
                     NSTINFHIIFIYGTVLVTLCLLRYIDEYPGRRLEHSFFLDLKLKALEKMSKIDYLEYQ
                     MLGTGKLIQKIENGANAGRNIFFNFLLAVVRKIIPSILFSMVFIYR"
BASE COUNT          269 a          101 c           56 g          296 t
ORIGIN      
        1 tctataaata aaaaccatac taaataaaat tgatggaatt atttttctaa caacggctaa
       61 taaaaaatta aaaaatatat ttctgccagc attagctcca ttttcaattt tttgaattaa
      121 ttttccagtg cctaacatct gatattctaa gtaatctatt ttactcattt tttctaaagc
      181 ctttaatttc aaatctaaaa aaaaactatg ttctagtctt ctacctggat attcatcaat
      241 atatcttaaa agacataaag taactaatac agttccatag ataaaaatta tgtgaaaatt
      301 tatagtactg ttattaaatc tatctattag gctctgataa taattagcac taaaactatt
      361 taaaagttct attcctatac ctagtgctaa atatataaaa actaataacc tctctttttt
      421 tgctatttct attaaatatt tcataactgt acacctatcc ttatttattt tactttatta
      481 acttaaaata attttataga taaaatgttt ctttatttgt ttaaatttag tgtgattatt
      541 aatcatttca agttaacaaa acctaaagtt tttagcagta cagttctatt tgttagcaaa
      601 ttattatttt actcagtaca ttatgctaac atataatcta actgtactga gtaaatatct
      661 cgtttcatca tataaaatct cctttttata gtttaatata tatagttaaa tttactatat
      721 aa
//
LOCUS       DACTGW010000418          712 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.427, whole genome shotgun sequence.
ACCESSION   DACTGW010000418 DACTGW010000000
VERSION     DACTGW010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.427"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(25..>712)
                     /locus_tag="I9573_003679"
     CDS             complement(25..>712)
                     /locus_tag="I9573_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4798253.1"
                     /translation="KKSERVILNDYINTFFYNYNFEYNIKEFENGGDLLKKYPNNVDI
                     IFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRYLEKPLKYSL
                     FKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHTEKGIYTFKN
                     SMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSRYKIKLLREK
                     LNSCLVDVII"
BASE COUNT          248 a           99 c           53 g          312 t
ORIGIN      
        1 agaatataag ggcaaaaatt tacctcaaat tattacatct accaaacaac tatttaactt
       61 ttctctcaat aattttatct tataacgact tacaattatt gaattttgat ttataaatac
      121 aatattattt tctatatcta gcatatttac cttcattaaa tttataaggt agcttttatg
      181 acatctaaaa aaattatgtt cttctagatt tgattctatt tctttcatac tattcttaaa
      241 agtatatatt cctttttcag tatgtatagt taaatcattt tttaaaactt ctaaatataa
      301 aatatcatct acctttatga aatatatctt gcatttacat tttatagata aaatagcttc
      361 atttttgtag cattctttta aatatgatga tattgcttct ttaaatagag agtattttaa
      421 gggcttctct aaatatctat aaactcctat ctcataacct ttctttatgt atttatttga
      481 tgaggataca aatataattc ttacactttc atcaaatttt ttgatttctt ttgaaacatt
      541 aaatccattt tcatcattta agtctatatc cataaatatg atatcaacat tattaggata
      601 cttttttaat aaatcgcctc cattttcaaa ctctttgata ttgtattcaa aattgtaatt
      661 ataaaaaaag gtatttatat aatcatttag aattaccctc tctgattttt ta
//
LOCUS       DACTGW010000419          701 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.428, whole genome shotgun sequence.
ACCESSION   DACTGW010000419 DACTGW010000000
VERSION     DACTGW010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.428"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..625
                     /locus_tag="I9573_003680"
     CDS             <1..625
                     /locus_tag="I9573_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HAT4798254.1"
                     /translation="AGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPNLNNNTSTSE
                     IDISSSADISVIKLANKTEACVEEQIDFVIVVSNAGPENAQNVTLIDNVSDKLKKAVF
                     SLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNIAEVTSTTPDSNLN
                     NNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDFWC"
BASE COUNT          286 a           86 c          115 g          214 t
ORIGIN      
        1 tgctggagag ataagaaata tcataataag gggaatagta agtcaaacag caataggtac
       61 aattataaat acagcaacag taagttcaac tacacctgac ccaaatctaa ataacaatac
      121 ttcaacatca gaaatagata taagttcatc agcagatatt tctgtaataa aattagcaaa
      181 taaaacagaa gcatgtgtag aagagcaaat agattttgta atagtagtat caaatgcagg
      241 accagaaaat gctcaaaatg ttactttaat agacaatgtt tcagataagc ttaagaaagc
      301 agtattttca cttgatagag gtgtaagttt ccaaccatgg actggaagtc ttaatatagg
      361 tacactacca gctggaactt taagagttat tctattaaga ggaattataa aatcaacatg
      421 tcttgataga ttaacaaata ttgcagaagt tacttcaaca acaccagatt caaaccttaa
      481 caataatata tcaagagtgc aagtagaaat aaaacaatgt tgttgtaatg attgctgttg
      541 taacaactgt agtaattgtt gttgcaatga ttgtggttgt aaagattgtt gctgtaattg
      601 ttgtaaaaat gacttttggt gttgatttta taaaataatt ttgtatacct taaattaaac
      661 tgaaataaaa aataaattca catatgagta aaatatttta t
//
LOCUS       DACTGW010000420          700 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.429, whole genome shotgun sequence.
ACCESSION   DACTGW010000420 DACTGW010000000
VERSION     DACTGW010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.429"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..423
                     /locus_tag="I9573_003681"
     CDS             <1..423
                     /locus_tag="I9573_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAT4798255.1"
                     /translation="TISKKYKMRSVNPLKAWVKEYNLHKEIKETPKGGLFMETRKTTL
                     DERVEIVKDCIEHDFNYNLIAKKYFVSYSQVRRWTLKYKENGIVGLEDRRGKAKKEDE
                     LTQLDEIKLELKIVKAEKHKLQLENAFLKKLKNIERGW"
     gene            417..>700
                     /locus_tag="I9573_003682"
     CDS             417..>700
                     /locus_tag="I9573_003682"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAT4798256.1"
                     /translation="MVITLSKNENKYKAIQSLFNEKKASLMQLCEIAKVNRSGYYKWL
                     NRDKSNLELENIKLATLITNIYEEKKGVFCSLRMTLQLNREYKLNVNHKRV"
BASE COUNT          307 a           78 c          126 g          189 t
ORIGIN      
        1 actatctcaa aaaaatataa aatgaggtct gttaatccac taaaagcttg ggttaaggag
       61 tataatcttc ataaggagat aaaagaaacc ccgaagggag gactctttat ggagactaga
      121 aaaacaacat tagatgaaag ggttgaaatt gttaaagatt gtattgagca tgattttaat
      181 tacaatttaa tagcaaaaaa atactttgtt tcatacagcc aagttagaag atggactctt
      241 aaatataaag aaaatggtat agttggttta gaagaccgaa gaggtaaagc aaaaaaagaa
      301 gatgagttaa ctcaattaga tgaaataaag ttggaattga aaattgtaaa agcagaaaaa
      361 cacaaattac aactggagaa tgcattctta aaaaagttaa aaaacataga aagagggtgg
      421 taattacact atccaagaat gaaaataaat ataaagctat tcaatcctta ttcaatgaaa
      481 aaaaagcttc gcttatgcaa ttatgtgaaa tcgctaaagt aaatcgttct ggatactata
      541 aatggctcaa tcgagataaa agtaatttag aacttgaaaa cattaaatta gcaactttga
      601 taactaatat atatgaagaa aagaagggag ttttttgctc tttaaggatg accttgcagt
      661 taaacagaga gtataaactt aatgtcaatc acaagagagt
//
LOCUS       DACTGW010000421          660 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.431, whole genome shotgun sequence.
ACCESSION   DACTGW010000421 DACTGW010000000
VERSION     DACTGW010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.431"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            266..571
                     /locus_tag="I9573_003683"
     CDS             266..571
                     /locus_tag="I9573_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798257.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
BASE COUNT          278 a           60 c           84 g          238 t
ORIGIN      
        1 gcttataagc cagaaaatga atactttaat agattaaaaa atacatattt gtgaattata
       61 gaatgaataa ctccataaac tagaaaattt ataataatat ctatatgaaa attagaatta
      121 ttttatcaca aaaaaattaa gttaaataaa tatgaaatta cttccaaata tacttccaaa
      181 aacatgtaat cgcatatata atataattat aaaaataata aatattttta tagtagtagt
      241 taattattaa taaggagggg tcaaaatgcc tagcatgatt atggaagttg taggagtact
      301 atttatagct attggattaa tcttgacatt cattgaaata aaaggaaatt tcccttcttt
      361 ttatggtttt ggtcaaaatt ctatagggaa aaatgctgta ggtattatag cagcaggaat
      421 tgctttaatt ttctttagaa atggcataga tagcatagat tctatagcta cttatggtgt
      481 atttttagca atagaacttg ttgtaatagt tgcatatttc ttaataagaa aaaaatttac
      541 taaagaagat aaacatttaa gaactaatta actaaattat ctactataaa attggaaaat
      601 taaaaacaaa taaaagtttg tttttaatta tttaaaatcc caatttgatt atttttaaag
//
LOCUS       DACTGW010000422          653 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.432, whole genome shotgun sequence.
ACCESSION   DACTGW010000422 DACTGW010000000
VERSION     DACTGW010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.432"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            37..>653
                     /locus_tag="I9573_003684"
     CDS             37..>653
                     /locus_tag="I9573_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HAT4798258.1"
                     /translation="MTLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPEYLNVWELQ
                     GITINSKNNPKTLNGQDLEKMGLNLKDYNVTQECIIEDITSRKDVNKYLRKTSAPITE
                     LTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPILLVEKNNV
                     PNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNG"
BASE COUNT          285 a           92 c          109 g          167 t
ORIGIN      
        1 agtgtcaaat ttaaagaaaa accagatgca gatagcatta cattaaagta taaatgctat
       61 gacatgccat tggatacaac tctaaattac aatcaatcaa ctggggcata tgaaggaact
      121 atcaattata accaagaccc agaatatcta aatgtttggg aactacaagg gataacaata
      181 aacagcaaaa ataatcctaa aactttaaac ggacaagacc tagaaaagat gggattaaat
      241 ttaaaagact ataatgtaac acaggaatgt ataattgaag acataacttc tagaaaagat
      301 gtaaataaat atttgagaaa aacttctgca cctattacag aacttacagg aagtgataga
      361 tatgaaacag cagttaaaat aagtaaagag ggctggaaaa atggttcaga taaagtagtt
      421 ataataaatg gagatgtaag tatagatggc attatatcaa ctccgctggc aaccacatat
      481 aatgctccaa tacttttggt tgaaaaaaac aatgtgccta atagtgtaaa atcagaatta
      541 aagcgcctaa accctaaaga tataattata attggagatg agaatgctat ttctaaaact
      601 actgctaatc aaattaaatc gactgtaaat gctagtcaaa cacgtttaaa tgg
//
LOCUS       DACTGW010000423          653 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.433, whole genome shotgun sequence.
ACCESSION   DACTGW010000423 DACTGW010000000
VERSION     DACTGW010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.433"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            37..>653
                     /locus_tag="I9573_003685"
     CDS             37..>653
                     /locus_tag="I9573_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HAT4798259.1"
                     /translation="MTLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPEYLNVWELQ
                     GITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLRKTSAPITE
                     LTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPILLVEKNNV
                     PNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKG"
BASE COUNT          289 a           82 c          107 g          175 t
ORIGIN      
        1 agtgtcaagt ttaaagaaaa accagatgca gatagcatta cattaaagta taaatgctat
       61 gatatgccat tggatacaac tctaaattat aatcaatcaa ctgagtcata tgaaggaact
      121 atcaattata ataaagaccc agaatattta aatgtttggg aactacaagg gataacaata
      181 aacagcaaaa ataatcctaa aactttaaac aaacaagaac tagaaaagat gggattaaat
      241 ttaaaagact ataatgtaac gcaggaatgt ataattgaag atataacttc tagaaaagat
      301 gtaaataaat atttaagaaa aacttctgca cctattacag aacttacagg aagtgataga
      361 tacgaaacag cagttaaaat aagtaaagaa ggctggaaaa atggttcaga taaggtagtt
      421 ataataaatg gggatgtaag tatagatggc attatatcaa ctccactagc aactacatat
      481 aatgctccaa tacttttggt tgaaaaaaat aatgtaccta atagtgtaaa atcagaatta
      541 aagcgtttaa atcctagaga tgtaattata attggagatg agaatgctat ttctaaaact
      601 actgctaatc aaattaaatc gactgtaaat gctagtcaaa cacgtctaaa ggg
//
LOCUS       DACTGW010000424          623 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.435, whole genome shotgun sequence.
ACCESSION   DACTGW010000424 DACTGW010000000
VERSION     DACTGW010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.435"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..371)
                     /locus_tag="I9573_003686"
     CDS             complement(<1..371)
                     /locus_tag="I9573_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017353736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAT4798260.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKTEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLEN"
BASE COUNT          226 a           85 c           58 g          254 t
ORIGIN      
        1 taattttcaa gtgaaaatac tttatggtat ccaaatttct cataaaattt aggtgcttga
       61 aaactgaatg tatctaaaaa aacatattta caacctcgtt ctttagcaat tttttcagtc
      121 ttatataaaa gttgacttcc agtaccttta tatctaagct tctcactaac ccataagtat
      181 tttatagaaa gccaattacc atgagtaaca cctataaaac ctgcaacaat tttacctgct
      241 ttatctcgaa tatatattcc taaatccact gggtttttat cttcaagtct agcaagatta
      301 tattttaata atccttgaaa aatagtatct tcatcttgtt tttctatttt ttctgtaatt
      361 tctaattcca tatttgaact ttctccttgt ataatctttt gtatattggt attttttaat
      421 aaaatcagta gaaagaatat agtttaacat tattctaata ggcatttatg ctaatgtcaa
      481 actaataata cttattctta attttatatt gttgatatta aattttttaa aataaaaatg
      541 tagtttaagt tatataaaaa gaaatcatca ataaatttag taaacctaaa ttgttgaata
      601 ttaaaataag ttaatttaat aca
//
LOCUS       DACTGW010000425          620 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.436, whole genome shotgun sequence.
ACCESSION   DACTGW010000425 DACTGW010000000
VERSION     DACTGW010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.436"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
BASE COUNT          265 a           41 c           86 g          228 t
ORIGIN      
        1 tataatattt caagtttgta taatgtaaca attaaaccaa aagactcatt gtatatttga
       61 aattaagttt tttatagttt ataggtgttt tatgtggatt acgaatgata tagtatcgat
      121 acattattta atcgattctt attccaagaa acaattaatt tatagaaatt cttaattgtt
      181 acaatataaa tatctagata agttataaga tactgataaa taatttataa agtttattaa
      241 tctaatattg tgtgtgaatg ttaagtggtc aagtagtcta aaaaatgaaa aagatttaaa
      301 aaagtgtttt aactaaacac ataagtgtta aattcatgta taagtatagg ttagatacat
      361 aagaaataat tatgtaatat ctatctttgt aaaaaaatat taaattttgt aatttgtgga
      421 taaatatgtt tataatgtgg ataatattta atgtaataag ttaaagcaat atgaatcaac
      481 tgtttataat gtggataata ttataaatgt atgaataatt gcagtattta aaaacaaaaa
      541 aagttaaaaa agttgttgac tctaaaagaa aaagttggta tagtaatact tgtcctcaag
      601 aaaagggcaa acaaataaag
//
LOCUS       DACTGW010000426          620 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.437, whole genome shotgun sequence.
ACCESSION   DACTGW010000426 DACTGW010000000
VERSION     DACTGW010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.437"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(121..600)
                     /locus_tag="I9573_003687"
     CDS             complement(121..600)
                     /locus_tag="I9573_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAT4798261.1"
                     /translation="MPNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTISGILMFILGVSYFIIIKSLIEVLSSSEHSLFVKDNVKRFRIIGYLLLLNSIMEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          243 a          104 c           49 g          224 t
ORIGIN      
        1 ttataatctt ttaattttag cattatagtt tatgtattat accctaatat attttatttt
       61 aaattactag ttaatcactc ttttctggtt atttatctcc tatatattaa agtgtcacac
      121 ctaaatagtt aaatcattat cctcttttat ctttattgcc tttacaaatc catctgcaat
      181 tacaaaactc ataagagaag ttataaaata tacaaatact ggaacagtaa aataaaatcc
      241 aaatcctaaa tctaggaatc tcattccttt tccagtagta ccaaaagttg aaataaactc
      301 cattatagag tttaaaagca aaagatatcc aataatccta aatcttttta cattatcttt
      361 tacaaataag ctatgttcag aactacttaa tacttctatc aatgatttta taatgataaa
      421 atatgataca cctaaaataa acattaatat tccagaaata gtgccttgta gagttgcttc
      481 tccactaaaa cagaggtaca taaaactttt aaatcctccc acaacaatta ctaaccctaa
      541 aagaaaaaat aaaatttgta atataccact tagtaaattt tttataagtt tattaggcat
      601 cataccaact cctttaaaat
//
LOCUS       DACTGW010000427          591 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.440, whole genome shotgun sequence.
ACCESSION   DACTGW010000427 DACTGW010000000
VERSION     DACTGW010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.440"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     regulatory      complement(386..475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          229 a           89 c           54 g          219 t
ORIGIN      
        1 tatcatagat taacataaat gtctatttaa ttataatttt tagttaacat ttttatttat
       61 tcttaatatg aaaataaatt taaacatttt atattaccaa tatattaatg taaatgtaga
      121 taaaactaaa aagggttttc tagaagaaaa tctgtctaat tatacttact taccaatacc
      181 atcacttgat gcaagcaaga ttccacaact ttttctccgt actctaatat aaaatcatct
      241 aactgtgtat ctataaaatc acaataatca tatgaactac caatataagc agttagcata
      301 tgtgtacatt tatccatttt atctctaaac ataatgtatt tatcccccta aaaaactttc
      361 atgaaaaaca aaaagaaaat atctttttgg caactggctg acataactta ttttataagc
      421 gttagtccct atagctttgc gtcactaaat ttctctagtt ttgccgattt agttttattc
      481 tacagattaa taatacaata gaattagcta ttattcaaca tatctagctg aaaaattaat
      541 aaatttatat atgaacgtta aattatttga aaaaataaaa agcccctctg t
//
LOCUS       DACTGW010000428          573 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.441, whole genome shotgun sequence.
ACCESSION   DACTGW010000428 DACTGW010000000
VERSION     DACTGW010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.441"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(117..>573)
                     /locus_tag="I9573_003688"
     CDS             complement(117..>573)
                     /locus_tag="I9573_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAT4798262.1"
                     /translation="SHIYSNLLDNFFDKNLFIVNPKIKDKDELIKLMCNKLTQSEIVF
                     PSFAESVFYRESLSSTNIDDFLAIPHPMELSSIRTKICISILNEPIYWSEDSTVKLIM
                     MLAINKDDYIKINSIYDILLKIIHDNDIRDSISNCNDFDNFLSIIKSIV"
BASE COUNT          238 a           67 c           57 g          211 t
ORIGIN      
        1 taataattat aatcaataca aaataataac aaattataca aaacttactt atactattta
       61 tgctaaaaac aaaaaatatt ttattaaagt ttttaaaact tatgtaatat aaaaatctat
      121 actatagact tgattatgga aagaaaatta tcaaaatcat tacaattaga tatactatct
      181 ctaatatcat tgtcatgaat tatctttaaa aggatatcat aaatggagtt tatttttata
      241 taatcatctt tatttatagc taacatcatg attaatttaa cagtagaatc ttcactccaa
      301 tatattggtt cattcaatat ggaaatacaa atcttagttc ttatagagct taattccata
      361 ggatgaggta tagctaaaaa atcatctata ttggtagaag ataaactttc tctgtaaaat
      421 acagattcag caaatgatgg aaatacaatt tcactttgag ttaatttgtt gcacatcaac
      481 tttataagct catctttatc tttgatttta ggattaacaa taaataaatt tttatcaaag
      541 aaattgtcca aaagattact ataaatatgg gaa
//
LOCUS       DACTGW010000429          565 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.442, whole genome shotgun sequence.
ACCESSION   DACTGW010000429 DACTGW010000000
VERSION     DACTGW010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.442"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>565
                     /locus_tag="I9573_003689"
     CDS             <1..>565
                     /locus_tag="I9573_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAT4798263.1"
                     /translation="KKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELDDHLGYDRY
                     GNKNKNTKNSRNGYRNKNVKSDFGEITLNIPRDRNGDFEPKVISNYKNDISGIENQVI
                     GMYSKGMSTRDISSHLKSIYGIDISHSLVAKITDRVLPLAHEWQNRPLDMVYPIVFMD
                     AIHYKVRSEGRIINKAAYIAIGVNLEGI"
BASE COUNT          232 a           68 c           99 g          166 t
ORIGIN      
        1 aaaaaactta ttgaagaata tgatgttaaa acaactacag atattcaaga tatgctaaaa
       61 gatttatttg cgagtacaat tcatcaaatg ttagaagcgg agcttgacga ccatttaggt
      121 tatgatagat atggcaataa gaataaaaat actaaaaact ctagaaatgg atatagaaat
      181 aaaaatgtta aatcagattt tggagaaata actttaaata ttccaagaga ccgaaatgga
      241 gattttgaac caaaggtaat tagtaattat aaaaatgata tatcaggaat agagaaccaa
      301 gtgattggaa tgtactcaaa aggcatgtct actcgagata tatcaagcca ccttaagagt
      361 atttacggaa ttgatatatc tcattctcta gtagctaaaa ttactgatag agttctacct
      421 ttagctcatg aatggcaaaa cagacctctt gatatggttt atccaattgt atttatggat
      481 gctatacatt ataaagttag aagtgaaggt agaataataa acaaagctgc atatatagcc
      541 ataggtgtta acttagaagg tataa
//
LOCUS       DACTGW010000430          525 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.444, whole genome shotgun sequence.
ACCESSION   DACTGW010000430 DACTGW010000000
VERSION     DACTGW010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.444"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
BASE COUNT          200 a           43 c           65 g          217 t
ORIGIN      
        1 gtactaattg gaataatttt aactttatta tagattattt taatgccatt agtactgatg
       61 gtgtatttta aaattggtat aagcattaca aatacgaata tgccaactgt aatatgtaat
      121 ataagtagct atatacatat gtgctgtccc atatattgta tcattgttta tattaaaaag
      181 attatgtaaa ttggtatcaa tcaataaatc attttcagaa gaaataccta aaaacttaaa
      241 aaatacctat atgtgtattt agttagattg gagattttta catttaattt tatagtgaca
      301 atattatatt attgttatga tgtctattta tatgcaatta ctataatatc agaacttata
      361 gtatcatttg tttcattatc aataaggttt tttactttgt tttgtcaatt ttattaaaaa
      421 aagttattga tataaaagat aagaaggata aaactatatg acagatttca aacataagag
      481 tggatattta tataatagat attttatagt taattttatt agatt
//
LOCUS       DACTGW010000431          525 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.445, whole genome shotgun sequence.
ACCESSION   DACTGW010000431 DACTGW010000000
VERSION     DACTGW010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.445"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(260..376)
                     /gene="rrf"
                     /locus_tag="I9573_003690"
     rRNA            complement(260..376)
                     /gene="rrf"
                     /locus_tag="I9573_003690"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(385..458)
                     /locus_tag="I9573_003691"
     tRNA            complement(385..458)
                     /locus_tag="I9573_003691"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Gly,seq:tcc)
BASE COUNT          206 a           89 c           62 g          168 t
ORIGIN      
        1 ttaacataaa tttctacttt gttttttaac cttttaattt ttgaaattat atcaaagtgt
       61 ttttacccaa aattataaat aattacataa aaaataaact tctaaaaaaa ttataaaaac
      121 aaaaaataaa aaaattaaat atattgaata tatttaatta tcttcactat atcatttatt
      181 gccaaaaaaa tctataaaag acaaataaaa acatataaca attgtaggtt tattaaattt
      241 aatacaacaa aaaaagatta cgtggctacg tcctactctc ccaggcagtt tcccaccaag
      301 taccatcagc actagagagc ttaacttctg tgttcggaat gggaacaggt gtatcctctc
      361 tgctattgta accacataat cttttggagc gggtgaaggg gatcgaaccc tcacagctgg
      421 cttggaaggc cagaactcta ccattgagct acacccgcat taaattttta taaattagta
      481 aaactaattt tttagagtta gcaccctaaa aactacatat atatc
//
LOCUS       DACTGW010000432          504 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.446, whole genome shotgun sequence.
ACCESSION   DACTGW010000432 DACTGW010000000
VERSION     DACTGW010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.446"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
BASE COUNT          236 a           38 c           67 g          163 t
ORIGIN      
        1 ttataatcag tattataaat ccattacaag tagaagcaga agctattatt gagtctagtg
       61 tatacatttc ttcaataaaa gagaattata catatgataa tactaaattt aaaactatta
      121 aatctaaaag tagacataaa agtaggaaat ataataaaag taaagtaaag ttgaaaaaac
      181 gaagtataag aactaaaaag tatggatatc aaactataaa taaatataaa agacgaaatg
      241 caagaagaca taatatccta acaaaattag tattgtccat aatagtcata tttttggtta
      301 tagttttagt agtgctaata tttattaaaa gaaagaagaa aaaattttac taaaaataat
      361 taaaagaata tcatttttct aatcattggt atctatttaa taatatgatg taattaaaat
      421 ggagtaatat tctgtttcaa aggaggcata taaatacagg aatgagtatt aaatgatggt
      481 taattaagta aatttattga taaa
//
LOCUS       DACTGW010000433          469 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.448, whole genome shotgun sequence.
ACCESSION   DACTGW010000433 DACTGW010000000
VERSION     DACTGW010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.448"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..419
                     /locus_tag="I9573_003692"
     CDS             <1..419
                     /locus_tag="I9573_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798264.1"
                     /translation="EYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHYWDEKVATEK
                     TFINGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVE
                     LTNDSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT          205 a           49 c           68 g          147 t
ORIGIN      
        1 ctgaatatta tgaagtaaat ttcaataatt atttatatat tattaccagg ttttcattca
       61 ttccagatgg aactgagata ttttttactt gggaagaaat ccattattgg gatgaaaaag
      121 ttgctaccga gaaaactttt ataaatggcg aaacattagt ggattcatat aactttccaa
      181 ataatgaagt gaaagactac atacaaaagc gagaatcatt atatatagaa acaaaaaaat
      241 attatgaaga aaaaggatta aaaattgaga gacatgcact cggttcagaa gatggtgaat
      301 atgttgaact tacaaatgat tctatacttt atttactaga tatagaagca gttgaaataa
      361 gagaacaatc aaaaaatata gaagaatttc tatataagta caaaaaacgt tttaaataat
      421 ttatatttta aaatacataa caatacattg agtcatttaa cgaaatata
//
LOCUS       DACTGW010000434          463 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.449, whole genome shotgun sequence.
ACCESSION   DACTGW010000434 DACTGW010000000
VERSION     DACTGW010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.449"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            178..294
                     /gene="rrf"
                     /locus_tag="I9573_003693"
     rRNA            178..294
                     /gene="rrf"
                     /locus_tag="I9573_003693"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            301..375
                     /locus_tag="I9573_003694"
     tRNA            301..375
                     /locus_tag="I9573_003694"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Asn,seq:gtt)
     gene            380..454
                     /locus_tag="I9573_003695"
     tRNA            380..454
                     /locus_tag="I9573_003695"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:413..415,aa:Glu,seq:ttc)
BASE COUNT          145 a           83 c          108 g          127 t
ORIGIN      
        1 aatgtattta gcttatcgat actaataggt cgaggacttg accaatatta tttgatgtta
       61 ttcattaaga tatatgtgca gtttttagag tgttaactct aaaaaaaaat aaaaaaagta
      121 ttgacaaaaa tagtaaaaaa tgttaatatt atacttgtcc tagcaacaag aaaaaaatgt
      181 ggttataata gcagagaaga tacacctgtt tccattccga acacagaagt taagttctct
      241 agcgctgatg gtacttggtg ggaaactgcc tgggagagta gggcatagcc acgtgaatgt
      301 tccaaggtag ctcaacggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc
      361 ccacccttgg agccattatg gctcgttggt caagaggtta agacaccgcc ctttcacggc
      421 ggtaacaggg gttcaattcc cctacgagtc accatttgtg gga
//
LOCUS       DACTGW010000435          454 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.450, whole genome shotgun sequence.
ACCESSION   DACTGW010000435 DACTGW010000000
VERSION     DACTGW010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.450"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..373)
                     /locus_tag="I9573_003696"
     rRNA            complement(<1..373)
                     /locus_tag="I9573_003696"
                     /product="23S ribosomal RNA"
BASE COUNT          120 a          109 c           73 g          152 t
ORIGIN      
        1 aggactatta ccttctgtgg tgggtctttc caaacctctt caattacgat acctcttcgt
       61 tatgatatgt ccgcaacccc aataaagaaa actttattgg tttgggctaa tccgcgttcg
      121 ctcgccgcta cttacggaat cgaatttctt tctcttcctt caggtactta gatgtttcag
      181 ttccctgagt tcccctcatt aagctatgta ttcacttaat gatacttaga cattactcta
      241 agtgagtttc ctcattcgga aatcttcgga tcaaagttta cgtgcaactc cccgaagctt
      301 atcgcagctt atcacgtcct tcatcggctc ctagtgccaa ggcatccgcc ctgcaccctt
      361 aataacttga ccagttaaaa aggtttgata gattagaagc tatcaacttc gataatttta
      421 aaaagttatc agctttatat attacaaatt agat
//
LOCUS       DACTGW010000436          428 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.451, whole genome shotgun sequence.
ACCESSION   DACTGW010000436 DACTGW010000000
VERSION     DACTGW010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.451"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..177
                     /locus_tag="I9573_003697"
     CDS             <1..177
                     /locus_tag="I9573_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798265.1"
                     /translation="TYKTAIDEAQAILDKADATQKEIDDAVTALNTATATFEAGKVPT
                     TIALMLSRILGFMK"
BASE COUNT          201 a           48 c           50 g          129 t
ORIGIN      
        1 acatataaaa cagcaataga tgaagcacaa gcaatattag ataaagcaga tgcaacacaa
       61 aaagaaatag atgatgcagt aacagcatta aacacagcaa cagctacatt tgaagcaggg
      121 aaagtaccta ctacaattgc attaatgtta tcaagaatac taggatttat gaaataagaa
      181 ataaaaagca aattatttaa taaaatttat atatacttta aatagaaaat ttaatttaag
      241 gctgtgagta aaatttttaa aaataaattt atacacagcc tttttatcat gtaaagttaa
      301 ttgatatagt aattttagat aatttagaga tataaaattt aaaatgttag ataaagttca
      361 aagtataacc taatatcaaa acttcacata attttcatat taattatcaa ttaaattcac
      421 acacaaga
//
LOCUS       DACTGW010000437          420 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.452, whole genome shotgun sequence.
ACCESSION   DACTGW010000437 DACTGW010000000
VERSION     DACTGW010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.452"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>420
                     /locus_tag="I9573_003698"
     rRNA            <1..>420
                     /locus_tag="I9573_003698"
                     /product="23S ribosomal RNA"
BASE COUNT          106 a           97 c          130 g           87 t
ORIGIN      
        1 cgcctcccaa aaggtaacgg aggcgctcaa aggttctctc agtacggtcg gaaatcgtac
       61 gtagagtgta aaggcaaaag agagcttgat tgcaagacat acaggtcgag caaggatgaa
      121 aatcggactt agtgatccgg tggttctgcg tggaagggcc atcgctcaac ggataaaagc
      181 taccctgggg ataacaggct tatctccccc aagagtccac atcgacgggg aggtttggca
      241 cctcgatgtc ggctcatcac atcctggggc tgtagtaggt cccaagggtt gggctgttcg
      301 cccattaaag tggtacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc
      361 gtcgcaggcg taggaaattt gagaagacct gtccttagta cgagaggacc gggatggacg
//
LOCUS       DACTGW010000438          385 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.454, whole genome shotgun sequence.
ACCESSION   DACTGW010000438 DACTGW010000000
VERSION     DACTGW010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.454"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(170..370)
                     /locus_tag="I9573_003699"
     CDS             complement(170..370)
                     /locus_tag="I9573_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAT4798266.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
BASE COUNT          130 a           57 c           36 g          162 t
ORIGIN      
        1 tttaagtaca tactttataa atatattttt gtcagattta ttttataaaa agtatttaga
       61 ctaagtatta tttttaagta ttttcttgtt gaagttattt tttccttttt tacaaaatgc
      121 gtaaataaaa aaaagactct taaaagagtc tttccgaaaa atctatatat tataatatag
      181 ttatattttc tgcttgagga cctctagcgc ctttaactat atcaaagctt actttttgac
      241 cttcttctaa tgatttaaat cctgaagttt gtatagctga gaaatgagca aatacgtcat
      301 ctccactttc cattgatata aatccaaatc ctttttcatt attaaaccat tttactattc
      361 cattattcat taaaaatact cctat
//
LOCUS       DACTGW010000439          381 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.455, whole genome shotgun sequence.
ACCESSION   DACTGW010000439 DACTGW010000000
VERSION     DACTGW010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.455"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(22..204)
                     /locus_tag="I9573_003700"
     CDS             complement(22..204)
                     /locus_tag="I9573_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798267.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMVKYGENIVD
                     SNLHSIMMLLCKWGLS"
BASE COUNT          142 a           56 c           41 g          142 t
ORIGIN      
        1 acaaaaaagt ttcttaaaat gctaagataa tccccattta caaagcaaca tcattatact
       61 gtgtaaattt gaatcaacaa tgttttcacc atatttcacc ataaaatctt ctaattgtgt
      121 gtcaataaat gaataatatc cacttatact atcgatgtat gcagtcatta agtgtataca
      181 ttcatcactt ttatctttat acatatatat ctccccctat atacaatata taaatttaga
      241 tactaaatcg tatcatatta aatagttaag tgtgtaacat ctatgcagtt atatatttat
      301 atccttatat taataactaa agtgagtttt gacaatagat tttgggaaac tggatgttca
      361 aagtgtaaac ttggatttta a
//
LOCUS       DACTGW010000440          364 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.456, whole genome shotgun sequence.
ACCESSION   DACTGW010000440 DACTGW010000000
VERSION     DACTGW010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.456"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="I9573_003701"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="I9573_003701"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="I9573_003702"
     tRNA            291..362
                     /locus_tag="I9573_003702"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DACTGW010000441          361 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.457, whole genome shotgun sequence.
ACCESSION   DACTGW010000441 DACTGW010000000
VERSION     DACTGW010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.457"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(87..162)
                     /locus_tag="I9573_003703"
     tRNA            complement(87..162)
                     /locus_tag="I9573_003703"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(127..129),aa:Gln,seq:ttg)
     gene            complement(170..246)
                     /locus_tag="I9573_003704"
     tRNA            complement(170..246)
                     /locus_tag="I9573_003704"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(210..212),aa:Arg,seq:tct)
     gene            complement(276..359)
                     /locus_tag="I9573_003705"
     tRNA            complement(276..359)
                     /locus_tag="I9573_003705"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Leu,seq:tag)
BASE COUNT          111 a           87 c           73 g           90 t
ORIGIN      
        1 attcggatac ttaaacttat tctaaaattg aacctaacta tatttaaaat aaaaatagtt
       61 caaatatagc ttagtaatac atcatttggc tggggatgca ggactcgaac ctacgcatac
      121 atgagtcaaa gtcatgtgcc ttaccgactt ggctaatccc caatatatgt ggtgagcgca
      181 cagggattcg aaccccggac acacgcctta gaagggcgtt gctctatcca gctgagctat
      241 gcacccacaa tttatatatg tatatcaaac aaaaatggtg cggatgaagg gagtcgaacc
      301 cccacgccaa tggcgctaga tcctaagtct agtgcgtctg ccaattccgc cacatccgcg
      361 c
//
LOCUS       DACTGW010000442          352 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.459, whole genome shotgun sequence.
ACCESSION   DACTGW010000442 DACTGW010000000
VERSION     DACTGW010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.459"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>352
                     /gene="tnpB"
                     /locus_tag="I9573_003706"
     CDS             <1..>352
                     /gene="tnpB"
                     /locus_tag="I9573_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAT4798268.1"
                     /translation="ELSRKTIGSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKE
                     NDIICIEDLQVKNMIKNHKLSRSISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQ
                     ICNKCGYKNEEVKDL"
BASE COUNT          155 a           38 c           61 g           98 t
ORIGIN      
        1 gaattatcga gaaaaacaat cggtagttta aatagaaaca aagcaagatt aaaagttgca
       61 agacttcaag aacatatagc taatcaaaga aaggattttc tacaaaaact atctactaaa
      121 ttaataaaag agaatgatat tatttgcata gaagatttac aagtaaagaa tatgattaaa
      181 aatcataaac tatctcgctc aatttcagat gtatcatggt cagaatttat tcgtcaatta
      241 gagtacaagg ctaattggta tggtagaaaa attgtaaagg taggtaaatt ctttgcaagt
      301 tcacaaatat gcaataaatg tgggtataaa aacgaggaag tgaaggactt aa
//
LOCUS       DACTGW010000443          352 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.460, whole genome shotgun sequence.
ACCESSION   DACTGW010000443 DACTGW010000000
VERSION     DACTGW010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.460"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     regulatory      97..187
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          148 a           37 c           51 g          116 t
ORIGIN      
        1 ttgtaattat tggtaactaa atttaaaaaa taaaatattt aattatacac aaaaaagata
       61 tttaaaaaat ttgttattta ttgtatactt aagttaaaac taaataggca aatctagaga
      121 aatctagtga cgcaaagcta taggggctaa ggtttgtata cacaaactat gtcagccagt
      181 tgccaaaaag agttctaggt atattgtata cgtaggaaga gtctaggatg actgcctttt
      241 tggtggtcat tttgttatgg aaaaaatcac attatgtagt aaaaaacacc tataataaca
      301 tattatacca aatataattt aaaataaaga taaattatct ataaaagtta ct
//
LOCUS       DACTGW010000444          349 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.461, whole genome shotgun sequence.
ACCESSION   DACTGW010000444 DACTGW010000000
VERSION     DACTGW010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.461"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
BASE COUNT          137 a           57 c           29 g          126 t
ORIGIN      
        1 aataatatat cgttttcata aattatatct ctcaaattgc tgtaaatctt tgtttttgtt
       61 aaatttattg attctaataa agataattga ttccttccat aaaagcctga aacacaataa
      121 ccttttgata aaaaataatt tgccaaagat gttccaactt ttccagaacc aataaagcct
      181 attttaatag tatcacctcc actttgtgat acaacatctt aatttctaat ctcattcctg
      241 aggatatgag ttacataaga caaaataatt aaaaacattt aaagtatagt tcttataaca
      301 agataccatc cataaatatc ttaccatttt cataaaaaat tgacaatta
//
LOCUS       DACTGW010000445          343 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.462, whole genome shotgun sequence.
ACCESSION   DACTGW010000445 DACTGW010000000
VERSION     DACTGW010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.462"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>343
                     /locus_tag="I9573_003707"
     rRNA            <1..>343
                     /locus_tag="I9573_003707"
                     /product="16S ribosomal RNA"
BASE COUNT           81 a           76 c           98 g           88 t
ORIGIN      
        1 tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa
       61 cccttgtctt tagttgccat cattaagttg ggcactctag agagactgcc agggataacc
      121 tggaggaagg tggggatgac gtcaaatcat catgcccctt atgcttaggg ctacacacgt
      181 gctacaatgg gtagtacaga gggttgccaa gccgtaaggt ggagctaatc ccttaaagct
      241 actctcagtt cggattgtag gctgaaactc gcctacatga agctggagtt actagtaatc
      301 gcagatcaga atgctgcggt gaatgcgttc ccgggtcttg tac
//
LOCUS       DACTGW010000446          341 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.463, whole genome shotgun sequence.
ACCESSION   DACTGW010000446 DACTGW010000000
VERSION     DACTGW010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.463"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            12..88
                     /locus_tag="I9573_003708"
     tRNA            12..88
                     /locus_tag="I9573_003708"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Pro,seq:tgg)
     gene            96..172
                     /locus_tag="I9573_003709"
     tRNA            96..172
                     /locus_tag="I9573_003709"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:130..132,aa:His,seq:gtg)
     gene            181..256
                     /locus_tag="I9573_003710"
     tRNA            181..256
                     /locus_tag="I9573_003710"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:214..216,aa:Lys,seq:ttt)
     gene            264..337
                     /locus_tag="I9573_003711"
     tRNA            264..337
                     /locus_tag="I9573_003711"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:296..298,aa:Cys,seq:gca)
BASE COUNT           78 a           79 c           96 g           88 t
ORIGIN      
        1 taatataaat tcgaggtgta gcgcagtttg gtagcgcaca tggtttggga ccatggggcc
       61 gggggttcga gtcccttcac ctcgaccaat ataaggtggg tatagctcag ttggttagag
      121 cgccagattg tggctctgga ggtcgtgagt tcgactctca ttatccaccc caattcatat
      181 ggtctattag ctcagtcggt agagcacctg acttttaatc agggtgtccc gcgttcgagt
      241 cgcggataga tcaccaaaaa ggtggcgaca tagccaagtg gtaaggcagt ggactgcaac
      301 tccttgatcc ccagttcgaa tctgggtgtc gcctccaaaa t
//
LOCUS       DACTGW010000447          332 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.466, whole genome shotgun sequence.
ACCESSION   DACTGW010000447 DACTGW010000000
VERSION     DACTGW010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.466"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>332
                     /locus_tag="I9573_003712"
     CDS             <1..>332
                     /locus_tag="I9573_003712"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAT4798269.1"
                     /translation="DLGDRSIVSYVIGKSNNNNLVFETFNKAIEVYPNAKPIFHSDRG
                     YQYTSRVFKSKLLTQGMIQSMSRVGHCIDNAPMEGFWGILKVEMYYLRKFDTYEQLVK
                     AIDDYIYY"
BASE COUNT          128 a           38 c           61 g          105 t
ORIGIN      
        1 ggatttaggt gatagaagca tagtttcata tgttattgga aaatcaaata acaataactt
       61 agtattcgaa acttttaata aagcaataga agtatatcca aatgcaaaac ctatctttca
      121 tagtgataga ggctatcagt atactagcag ggtgtttaaa tcaaaacttt taacacaagg
      181 catgatacaa agtatgtcga gagttggtca ttgtattgac aatgctccta tggaaggatt
      241 ttggggaatt ttaaaagtgg aaatgtacta tttaaggaaa tttgatacat acgaacaatt
      301 agtcaaagca atagatgatt atatatatta ct
//
LOCUS       DACTGW010000448          299 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.468, whole genome shotgun sequence.
ACCESSION   DACTGW010000448 DACTGW010000000
VERSION     DACTGW010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.468"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..>299)
                     /locus_tag="I9573_003713"
     CDS             complement(<1..>299)
                     /locus_tag="I9573_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4798270.1"
                     /translation="FLSVVFLLCTGSIILFKQLSNIYDDKERYIMLIKLGANNKDIEK
                     IISKQLKIIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIYFIGYFIYY"
BASE COUNT          121 a           36 c           28 g          114 t
ORIGIN      
        1 ataatatata aaatatccta taaaataaat gactagagtt atgataattg gtacaagtaa
       61 tgaccttggt ataaactttt gtgctataga catagcaaat aaattatgaa ctgtacctac
      121 aactaatggc attaaaaata taatttttaa ctgttttgat attattttct caatatcttt
      181 attatttgct ccaagtttta ttaacataat atatctttcc ttatcatcat atatatttga
      241 taattgttta aataagatta tactacctgt acataaaaga aatactacag acaaaaata
//
LOCUS       DACTGW010000449          209 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.479, whole genome shotgun sequence.
ACCESSION   DACTGW010000449 DACTGW010000000
VERSION     DACTGW010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.479"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            78..>209
                     /locus_tag="I9573_003714"
     CDS             78..>209
                     /locus_tag="I9573_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HAT4798271.1"
                     /translation="MISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFYTDDE
                     "
BASE COUNT           97 a           21 c           35 g           56 t
ORIGIN      
        1 atagtattac agacaacaca tataaatggt taaagagtga ggatttacaa aatgcttatt
       61 ttataggtgg acctcaaatg atatcaacaa atgttataaa taaggtaaat gatataacta
      121 aagataatgt tactaataat agagtatatg gagcagatag acacgaaaca aatgcaaacg
      181 taataaaaaa attctataca gatgatgag
//
LOCUS       DACTGW010000450          209 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.480, whole genome shotgun sequence.
ACCESSION   DACTGW010000450 DACTGW010000000
VERSION     DACTGW010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.480"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            78..>209
                     /locus_tag="I9573_003715"
     CDS             78..>209
                     /locus_tag="I9573_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HAT4798272.1"
                     /translation="MISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFYTEDE
                     "
BASE COUNT           94 a           20 c           39 g           56 t
ORIGIN      
        1 atagtattac agacaataca tataaatggt taaagagtga ggatttacaa aatgcttatt
       61 ttataggtgg tcctcaaatg atatcaacaa atgttataaa taaggtaaat ggaataacta
      121 aggatagtgt tactaataat agagtatacg gagcagatag acacgaaaca aatgcaaatg
      181 taataaaaaa attctataca gaggatgag
//
LOCUS       DACTGW010000451          206 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.denovo.482, whole genome shotgun sequence.
ACCESSION   DACTGW010000451 DACTGW010000000
VERSION     DACTGW010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.denovo.482"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..169)
                     /locus_tag="I9573_003716"
     rRNA            complement(<1..169)
                     /locus_tag="I9573_003716"
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c           39 g           60 t
ORIGIN      
        1 ttatcccgta ttagcatacc tttcggtatg ttatccgtgt gtacagggta ggttacccac
       61 gcgttactca cccgtccgcc gctctttacc gaagtaaatc gctcaacttg catgtgttag
      121 gcacgccgcc agcgttcatc ctgagccagg atcaaactct caaataaaag tttatcaggc
      181 tcagatacta ttgttatcaa aatatc
//
LOCUS       DACTGW010000452         6115 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.guided.209, whole genome shotgun sequence.
ACCESSION   DACTGW010000452 DACTGW010000000
VERSION     DACTGW010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.guided.209"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..329
                     /locus_tag="I9573_003717"
     CDS             <1..329
                     /locus_tag="I9573_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798273.1"
                     /translation="LKVYDEKKLSSESVFYIQSDKNELEKIFSKEVIKKMKKLNRNLD
                     VVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRSIQAPRLEYITNNVY
                     YFKGEL"
     gene            357..1295
                     /gene="blaCDD"
                     /locus_tag="I9573_003718"
     CDS             357..1295
                     /gene="blaCDD"
                     /locus_tag="I9573_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HAT4798274.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1639..2322
                     /locus_tag="I9573_003719"
     CDS             1639..2322
                     /locus_tag="I9573_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAT4798275.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2319..4979
                     /locus_tag="I9573_003720"
     CDS             2319..4979
                     /locus_tag="I9573_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAT4798276.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5160..>6115)
                     /locus_tag="I9573_003721"
     CDS             complement(5160..>6115)
                     /locus_tag="I9573_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4798277.1"
                     /translation="LSKHGYSKSTFWSKNFFYFLVISFGIISTMATVVYIIKHRTNKQ
                     KLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKTVGELRCSKEFALKERQTL
                     SDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRLTNQISRLERLVSSLLTLS
                     KLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTIEASPSVEFEIDINWTLEA
                     LLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGFAQEELPYIFNRFYRGKNA
                     SKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFYT"
BASE COUNT         2332 a          736 c         1021 g         2026 t
ORIGIN      
        1 ttttgaaagt ttatgatgaa aaaaagttaa gctctgaatc tgtcttttat attcaatctg
       61 ataaaaatga attggagaag atattttcta aagaagtaat aaaaaaaatg aaaaaactaa
      121 acagaaattt agatgtagtt aaaatttctg ggaatagaaa tttagtctat tttttctttg
      181 gagctgaatt acaatctatt gatggtattg tgatttttaa agatttaaaa catgttaaac
      241 caaagttaga ttttataaat agaagtattc aagctcctag attggagtat attacaaata
      301 atgtatatta ttttaaaggt gaactataaa cttatgtaga ggagatggta taaagtatga
      361 aaagaaaaaa taattttatt tggatagtta ttcttctagt tgttgtagct atgggagtat
      421 attatgtagg aagaaagcat aacaacacaa aacaaaaaca tgataagaat catataaact
      481 ctgaattaaa taaatccaaa gttgaaaaca atgataaaaa caaaaaagaa aaaattaata
      541 tggttgacta tagtgactgt tttgaaggta ttagtggagg agctattttt tataatacta
      601 aaaataaaga atataatata tataacaaag aattaattga aacaagaagg tcaccatgtt
      661 ctacttttaa aatagtttca actttaattg gtttagaaaa aggggtaata aactcaaaag
      721 aatcagttat gggatatgat ggtacagagt atccaaataa aaattggaat aaaaatctta
      781 gtttagaaga ggcatttaag gagtcttgtg tttggtacta caaaaaattg atagataaag
      841 ttgatgctaa atctgtccaa aacattttag atgatttaaa gtatgggaat tgcgatatta
      901 gcgaatggga aggagattta aaaaatggaa agggacattt aaatggattt tggttagaat
      961 cttcattaca aatatctcca aaagaacaag tgcaaacaat ggcaaaaata tttgagggag
     1021 atacaaactt taaaaaagag catattaata ttttaagaga tattatgaaa atagatgtaa
     1081 atgataaaaa cataaatgta tatggaaaaa ctggaacagg atttgatgaa aaaaataaac
     1141 gtgtggatgc atggtttgtt ggtatgctag agcgtgaagg tgacacctat tattttgcta
     1201 ttaaatcaga tgattccaat aaagaaatta cagggccaaa ggtaaaggaa attgcaatta
     1261 atataattaa gaaatattat agtgtaaggg aataaactta attttatttg taaatattat
     1321 ttacctatag tctgtaatat ttattaaata attttactaa attgagtcta tatagagcaa
     1381 aactccttta gtacataaaa tacttgctat tgattattaa attgaatacc tatagattta
     1441 aagttaatac cttttaaaga ctatgtaata tattaaaagt ataaataaaa tatattgcat
     1501 agtttttgtt ttatattata attttaaata atgtgcaaaa actaaacaat atttttaata
     1561 gtaatttata tttgtattca tgtcactata atgtcatttt aggttgctaa aatttaacca
     1621 taaaggagat gattgtatat ggaaatatta aaatgtgaaa atctcacaaa aatatatggt
     1681 tcaaatcaaa ccagagtgac agcccttaat aatgtaaatt tatcagttca aaaaggtgat
     1741 tttgtatcca ttgtaggagc atctggttct ggcaaatcta cattgttaca tctgcttggg
     1801 ggagtagata gacctacatc tggaaagatt tatgttgaag atacagaaat atcaagttta
     1861 aaggaagaag ctcttgcagt ttttagacgt agaaaagtag gtcttattta tcaattttat
     1921 aatcttatac ctacccttga tgttagaaaa aatatattat tacccatgtt acttgataaa
     1981 agaaaggtgg atgaggatag attctctgag attgtatcta tattgggatt atctgataga
     2041 ttaaaccatc ttccaagtca actttcaggt ggacaacaac aaagggtatc tatagctaga
     2101 agtttaatct atagaccagc tattttatta gcagatgagc caactggtaa tcttgataga
     2161 aaaaattcag aagaaattgt agacttgtta aacttatcaa ataaacgatt taatcagaca
     2221 atacttctta ttactcatga tgagaaaata gcactagagg caaatcgtat tgtaactatg
     2281 gaagatggag tgattgtctc tgaaaaggtg gtgaagaaat gaatattctt caagaataca
     2341 ccttagactt tgtacgacgc aataaacgta gtagtatagc tataatgtta gctatattac
     2401 ttactacaac catgatgtct tctttgtgtg gactgttgta taccatgtgg acagatttta
     2461 taagactttc aattgagaaa gaaggtaact ggcatggtga attgtttgac tatacttatg
     2521 gaagtgactt accattaatt gaaaattttt cttcagttga tgatgtaatg gtaaaaggac
     2581 aatggtatgt aggcaaaatt aatgatgaga aaagagatta tataatttat agaaatgcta
     2641 catcagagta ttggaattct atgccagaaa aggatactat aatagagggt cgtcgaccaa
     2701 ataatgctaa tgaaattgca ttatcaaaac aatattttga gaataatcca agtgtaaaaa
     2761 ttggggataa aattactctt ccaataggag atagaatcct aaatggaaag cctcttgaac
     2821 ccatagctca aaaatcgaaa ggggaaagtt ttaaaaaaat ttcagagact acattgacaa
     2881 tagtaggaaa ggtagatgtt tccacttcat cagtaatacc tgcatataca gctcttggat
     2941 atcttgatag aacaagtata aaacctaatg atagtataac tgtgtacttg cgatttaaaa
     3001 atataagaga cacatacaag gaacttccaa aacttgctaa atcattgggg tggaagacta
     3061 atgaatatgg aaaatacaat cttaaatata atagtaatta tcttttaaaa atgttagtct
     3121 tttccccaga acagaaagca tctatgagca gtatatcaaa attttcaact cctattatgt
     3181 atcttacaat agcaattttt acagtagctg tgtttgtaat ggtgatttac aacgcgtttt
     3241 cactatctgc aaatgctaga cttactcaat tagggatatt atctagtgtt ggtgcatcac
     3301 caaaacagat taaaatgtct gtagtgtttg aaggtttttt gcttacaatt ataccattgc
     3361 caataggttt atttttagga tggttattgt gtaataggct tatagtttat attaattcag
     3421 ttaattatca tactgatgtt ccagaagtgg tatttactta tggtattcct gcttttctgc
     3481 cagcagtttt acttacaata gtaacagtct ggatttctgc acttatacca gcacgaaaag
     3541 tatcaaagat atctcctata gaagcaatac gacaaggaga tagtgttaaa atcaaaaagt
     3601 cgcataaata ttcattagga aagatatttg gaatagaagg agaacttgca tcaaacgcac
     3661 tacgtgctcg taaaaaatct tatcgtactg caacaatatc tttaacatta tcttttttat
     3721 tacttacatg ttttttgcat ataattgcaa atcaagaagg agcaaaagct gtttatggaa
     3781 gtgataatta taaggaccaa agagatatag cagtttattt ggaaaatagt gaacctgtag
     3841 agtctgcttt cattgaaaaa ttagataata cagaaggtgt aaaaagttca ctatattatc
     3901 aaaaaatgtc tgcttccact tggctgactg aaaaagatgc atcaaaagaa cttgcgaaaa
     3961 atggaggatt taaaagtata gtttcatcta aaaaatactc tcctatagaa cgtgatggca
     4021 agtttcgtat aatttcaaat ataattgggc ttgatgataa gagttttaca gaatattgta
     4081 ggcaaatttc tgtagaccca aagttatttt atgatacaaa aaatccaacc tcaattgtat
     4141 ataataagga ggaagatatt aatcgtagta cacgtagaaa taaagtatat atagattatt
     4201 taaatctttc aataggagat aacattaaat taaatgaaaa agtatatgac gaagataaag
     4261 gggattatac ttacaacatg aaagtgggag ctattacaag cacacttcct aaaacagtag
     4321 atactagttc aaaatataca ttaatgcaaa tagtgccaat gtctatggtg caggaaatat
     4381 ctaaaaatta tgatgaagta agtaggttac gttcaaatag gctgatagga gtatttaaag
     4441 taaataatcg tgaggacatt cctagagtaa gagaaaaatt agagtccatt tataataaag
     4501 actatggttc aggagattat agtattgagg atgttttaga aagtgaaaaa caaaaggcta
     4561 gtggaataaa gaccatgaat ttaatagtag gctttattac aggtttattg gctatgattg
     4621 gattatcaaa tgtacttgct acagtgtcag gaaatactag aagtagacga caagagtttg
     4681 ctatgttgcg ttctgttgga ttatctccag aaggcataaa aaagatgctt gttttggaag
     4741 gtttgttctt aggaattaca ccattactac ttagtattcc tgtgcaaata ggaatagtgt
     4801 atgcttttct acgtataaat gaaatatatt ttatagagta tttaccattt gcacctatat
     4861 ctacaataat aggttttact atattgatac tttttattgt aatagcatca tatatgacag
     4921 gatacaagca gttgaagaat gaaaatatag tagaatctat aaaaaatgaa acaatataaa
     4981 caaaagctat ttgaacttat ttaaaggttt cgatttaaga ctacttacaa gtatagggat
     5041 agttaatttg tttgtttaat aaaaccttaa atgtaaatag aagtatatta ctaaaacaat
     5101 agataacata gaatatagat atagctggat tataggtatt ttacctataa tccagcattt
     5161 tatgtgtaaa atttaattat gaatcttgca ccaccattat ggcaattttc tgcatgtaga
     5221 gtaccatttt gacgttctat aatagacttt gatagagcaa gccctatacc aatactgtcc
     5281 ttactagcat ttttaccacg ataaaatcta ttaaaaatat atggaagttc ttcctgagca
     5341 aaccctttac cattatcttc tatgataatc tcaacatata gaggattttg actataataa
     5401 gcaactatat atccaccatc ttctatatgc tcactacaat ttttaagaat atttaaaaga
     5461 gcttctaaag tccaattaat atcgatttca aattctaccg aaggagaagc ctcgatagta
     5521 aatgtctgat gtttttctct taaagaacct tctataggct caattgcata agtaagaaga
     5581 gaatgtacat ctataagttt gctttcaaat attaaagtac ttgcatccaa ttttgataat
     5641 gtaagtaaag atgaaactag tctctcaagt cttgagattt gatttgtaag tctgttaata
     5701 taatctattt cttcatctgg acaattttct gaaagtaatt gtgccataag tgacatagaa
     5761 gtaattggag tcttaagttg atgagcaata tctgcaagat tatctgaaag agtttgtctt
     5821 tcttttaaag caaattcttt tgaacaacga agttccccaa ctgttttata tatctcatct
     5881 tcaaggtgag aaaattcatc ttcacatcgt aaaagtacag actcttttcc taaattcact
     5941 tcttctaagt attgagtaag attgtttatt ctactcagtt tttgcttatt tgttctatgt
     6001 ttgattatat atacaactgt agccattgtt gaaattatac caaacgatat cactagaaaa
     6061 taaaaaaagt ttttactcca aaatgtactt ttagaatatc catgttttga taaaa
//
LOCUS       DACTGW010000453         5488 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.guided.226, whole genome shotgun sequence.
ACCESSION   DACTGW010000453 DACTGW010000000
VERSION     DACTGW010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.guided.226"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..366
                     /locus_tag="I9573_003722"
     CDS             <1..366
                     /locus_tag="I9573_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAT4798278.1"
                     /translation="SLERDLNKFVYTVEGVDDNNEYKMKIDANTKDVLEDKTEKLDSE
                     DLNGVARKEKLDLNDIITPQQAMEIALKEQNGMVKEWSLDKDLDVTFYKIRIDKDKNE
                     YDIKVDSKKGTVLKVEKED"
     gene            474..3005
                     /locus_tag="I9573_003723"
     CDS             474..3005
                     /locus_tag="I9573_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAT4798279.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3439..4140
                     /gene="vanR"
                     /locus_tag="I9573_003724"
     CDS             3439..4140
                     /gene="vanR"
                     /locus_tag="I9573_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family DNA-binding response
                     regulator"
                     /protein_id="HAT4798280.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKAQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4190..5272
                     /locus_tag="I9573_003725"
     CDS             4190..5272
                     /locus_tag="I9573_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT4798281.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
BASE COUNT         2136 a          543 c         1021 g         1788 t
ORIGIN      
        1 agtcttgaga gagatttaaa taaatttgtt tatactgtgg aaggtgtaga tgataataat
       61 gaatataaga tgaaaattga tgctaataca aaagatgtat tagaagataa aacagaaaag
      121 ttagattcag aagatttaaa tggagttgct agaaaagaaa aattagattt aaatgacata
      181 attacacctc aacaagctat ggaaattgca ttaaaagaac aaaatggaat ggttaaagaa
      241 tggagtttag acaaagattt agatgttact ttttataaaa taagaataga taaagataaa
      301 aatgaatatg acataaaagt agattctaaa aagggaactg tattgaaggt agaaaaagaa
      361 gattagaaga tttaaactat ctttaaaata atttcttttt tgaataaatt ttgtttaaaa
      421 tataacaagc agggtataaa gagactaaat aactatttta ggaggagtac ggtatgtcat
      481 tgaaatcact aaaaataaaa gaaaatttat attgggtagg ttcacttgac cctgatttaa
      541 gagtatttga cataattatg tatacacctt atggaactac ttataattca tatgtattaa
      601 aaggaacaga aaaaacagtt ttgtttgaga cagttaaaga taagcatttt gacaattata
      661 ttgaaagatt aaatgattta aatatagatt ttgaaaaaat agactatatt gttgtaagtc
      721 atactgaacc agaccatgca ggaagcgttg aaaagctttt ggatttagct aaaaatgcaa
      781 aagtagtagc ttcagaaaca gcaattaaat atcttaagga aatcgtaaac aaagactttg
      841 aatatgttgc agttacagat ggagatacat tatcaattgg ggacaaaact ttagaatttt
      901 tctctgtacc tatgcttcat tggccagata ctatatacac atatataaaa gaggataaaa
      961 ctcttgtaac atgtgattca tttggtagtc actatagtaa tgataagata gtaaatacac
     1021 ttgatgaaaa tgaagaaaag gattacttgg atgctttaag atactattat gattgtataa
     1081 tgggtccatt taaaccatct atggtaactg ctatagaaaa gataaaagat ttagatatag
     1141 atactgtatg tccaggtcat ggtccagtat taactgaaaa tcctagaaaa attatagacc
     1201 tttattataa ctggagtgta aaggaacaaa taaagttaga aaaagaagtt acaatttgtt
     1261 atgtttctgc acatggatat acaaagatta tggcagaagc tataaaagca tatattgaaa
     1321 aaaatagcaa ctataaagta aatttatttg atgtaataga gcataagcaa gaagatattt
     1381 tggcaaaaat agcagtatca caaggtgtat tatttggaac accaacaata ttaggagatg
     1441 cgttaaagcc tatatgggat atattaatat cgttaaaccc agttcttcat ggtggaaaag
     1501 ttgcttcagt atttggttca tatggatgga gtggagaagg tatagaaaat gctatggaga
     1561 gaataagtca gcttagaatg acagcagtaa aaccttttgc tgttaacttt aaaccatcaa
     1621 atgaagagat tgataaatta cattcttata caggtaaatt cctagataag ttaaattcta
     1681 cttttggaag taaaaagaaa actaagaaat ttaaatgtgt aatttgtaat gaagtttttg
     1741 aaggtgatag tgctcctagt gtatgccctg tatgtggagc taaggaagac caatttatag
     1801 aagttgaaga agatgaagtt acttttagaa aagatactga tgaatatttc gtaatagtag
     1861 gaaatggagc agctggtttt tatgcagcag atgctataag aaaaagaaat aaaacttgta
     1921 agataactat gatttctaat gaagatgagt tgacttacta tagacctgca ctttctgatg
     1981 gtataaatga agaacttggt tcagatttct acatggaaga taaagattgg tatgataaaa
     2041 ataatatagt agtaatatta ggaacgaatg tagataaatt agatgaagtg aataaaacta
     2101 ttatagtgaa tgatggagct attaaatttg ataagcttgt aatagctaca ggaagtagaa
     2161 actttatacc accgattaag ggccatgatt tagaaaatgt gtttactctt agaaatataa
     2221 aagatttata tagtgtaaaa gaagcactag aaaaatctaa gaaagttgta gtgattggtg
     2281 gcggtttatt gggacttgaa gctgcatggg aatttagact aaaaggatta gaagtagtag
     2341 tagttgaagc catggatagc atactatcaa aacaacttga taaagagggt agtaaaatat
     2401 tagaacaatg tgtaagagat acaggaatag atgtaagatt gggtgtagca gttgatggta
     2461 ttgaaggtga tggaaaagct caaaaagttg tatttaaaga tggagatagt gttgactgtg
     2521 atatggtagt attttcaatt ggtgtaagag caaacactca aatggttcag gatacatctg
     2581 taaaaattga tagaggtatt gttgtagata agacattaca gacaaatgtt aaagatatat
     2641 atgcttgtgg agatgttgca caagtgggaa atattagttt agcaatatgg ccatcttcag
     2701 tagaaatggg aaaaatagca ggagcaaatg caagtggaga taatttgaca tttgaaagtg
     2761 aagtatatcc agtttcatta gatgcaatga atgtaaaagt atttagtatt ggtaatatac
     2821 aaaattttga taaagaaatt tcatcaaaag atgaaggtca aagaatatat aaaaaattat
     2881 ttatgaaaga tggttcatta gttggagcta tacttataaa tgatttaagc tgtactgtta
     2941 aattgataag attaatatct gaaaaaggtg attttgagga tattatgaag gctgatatac
     3001 tataatttaa atacattaaa ataaaaagga gttgtctcaa aattgatttt tttctaatca
     3061 aattttaaat tcaactcctt ttttgaattt ttatataaaa taacatggaa attttagtaa
     3121 tgtctgtggt tacatacata ggtttaattg ttttttatat ggattgtctc tggtataata
     3181 aattgaattg tgtttgactg atagatattt ttattgaaag ttaatacctg ttatgcgata
     3241 ataaggacgt gttaggggag tttttataat aacaattaat ctcagttttt gtgttccaat
     3301 agtagcatca acacattctg atgtagtagt tcttttctta taaaaattag ttacaaatat
     3361 gcttgtaatt atttgatatg taaatttacc aatattataa atgatgacct cgataataag
     3421 gagagtgaaa aataatctat gaacgaaaat attttgattg tggatgatga aaaagaaata
     3481 gcagatttaa ttgaagtata tttaaaaaat gaaggtttta cagtatacaa gttttataca
     3541 ggtaaggaag ctctagaatg tattgaatct aaagatttgg atatggctat tttagatatt
     3601 atgcttcctg atattgatgg atttcatatt tgccagaaaa ttcgagaaaa atactattat
     3661 cctatcatta tgttaacagc taaaattgaa gatatagaca agataatggg tttaaccata
     3721 ggtgctgatg attatataac aaagccattt aaccctttgg aagttgttgc aagagtaaag
     3781 gcacaattaa gaagatatat gcgatataat aattcttatg aacaacagtc gataattgtt
     3841 aatgagtatg atattaaagg attgattatc aataaagaaa cacataaatg tagtttattt
     3901 gggaaagaag tagcattaac accgattgaa ttttctattc tttggtattt atgtgaacat
     3961 caaggaaaag tggttccttc tgaagaattg tttgaagctg tatggggaga aaaatatctt
     4021 gataataata acacagttat ggctcatata gggcgattac gtgaaaaaat tcatgaacct
     4081 tctaaaaacc cgaaatttat aaaaactgta tggggagtag gttatacaat tgaaaagtaa
     4141 aaatattaaa gtcaatcctg actttactca agtgagaaat aaaattttta tgagaacagc
     4201 tttaatggca tttatagcca ttattttcat atggatttta tattctttta tattgtatgg
     4261 taagttttct atctggatag tatcattctt tgaaaatgct tttcgtatgg aatataaagc
     4321 tgcattgaat ttatatcagc agattttccg taatagcatg gagctcttca tgatactagc
     4381 tatggtgatt gtgtttttta taatattccg tgtttattta aattggttta caaaatattt
     4441 tagtgaaata aatcaaggta ttgactctct tatcaaagaa gatgttggag aagtggcttt
     4501 atctccagag ttattggcaa tagaaaagaa aataaactca attaaacata tacttgaaca
     4561 acgtaaattt gagacacaaa tggcagaaca acgtaagaat gaacttattg tttatcttgc
     4621 acatgattta aaaacaccac ttacatcagt tatagggtat ctgacactac ttcgagatga
     4681 aagtcagatt tctgaagaac tgcgaaaaaa atatttatct atttcgttgg ataaggcaga
     4741 gcgtcttgaa gatttaatta atgaattctt tgaaattaca aggtttaatt tatcaaatat
     4801 atcacttgaa tacagtacag taagcttaac tcgtatgctt gaacaactaa catatgagtt
     4861 taaacctact ttgatgagta agaatttaaa atatacactg aatgttacac caaatatgat
     4921 gataaggtgt gatgttaata aaatgcaacg tgtatttgat aacttattac gaaatgctgt
     4981 ttattatagt tttgaagata ctactattga aataacagct gtacaggagg atgattatat
     5041 aaagcttaaa tttataaatc atagcaatac gattccagag gaaaaacttg agcgtatttt
     5101 tgaacaattt tacaggttgg atacatctcg taattcaaac agtggtagag ctgggttggg
     5161 acttgctata gctaaagaaa tagtggagct tcataacggg acaataacag ctcatagcga
     5221 gaacaatata attaccttta aggttattat tccgattgta ggaaatttgt aagaatttaa
     5281 gtataaaaaa ataggaaatt ctttaggata aataaataaa gagcactctt gttattgata
     5341 taattactgt atcaaaacaa gagtgctttt attttgatac agtaattata tcaatagaaa
     5401 aatgtgaaaa tatattatgt ggaacagatg caattgtaag tgaaggttga ttggatttga
     5461 aatagaaaaa gtattttaac atgtttct
//
LOCUS       DACTGW010000454         5259 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.guided.233, whole genome shotgun sequence.
ACCESSION   DACTGW010000454 DACTGW010000000
VERSION     DACTGW010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.guided.233"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            44..1144
                     /gene="vanG"
                     /locus_tag="I9573_003726"
     CDS             44..1144
                     /gene="vanG"
                     /locus_tag="I9573_003726"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HAT4798282.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            1141..1947
                     /locus_tag="I9573_003727"
     CDS             1141..1947
                     /locus_tag="I9573_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAT4798283.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            1965..4103
                     /gene="vanT"
                     /locus_tag="I9573_003728"
     CDS             1965..4103
                     /gene="vanT"
                     /locus_tag="I9573_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HAT4798284.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            4417..5037
                     /locus_tag="I9573_003729"
     CDS             4417..5037
                     /locus_tag="I9573_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAT4798285.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
BASE COUNT         1923 a          586 c          889 g         1861 t
ORIGIN      
        1 tagattgtat aaaaataaat aaatcaacaa aggagaatca aagatgaata ataaaaaaat
       61 tgcagttata tttggtggaa actcaacaga atatgatgta tcattacaat cagcttttgc
      121 agtacttgag aatttgaata cggaaaaata tgatattata ccaattggaa ttacaaggga
      181 gggtaattgg tatcattata ttggagatta taataatatc cagaataaca cttggtttga
      241 aagggatact gatataattc cagttattgt ttcgcagaac cgtgtcaaaa atggtattat
      301 tgaattttta attcctgatg ctgtttctca gaatcatatt ccaaatagtg ctattgaatc
      361 atgtgaaaat gatattaaat acactaaact tgattttgta tttcctgtat tgcatggaaa
      421 aaatggagaa gatggaacag tgcaggggtt atttgaattg gcaggtatac aggttattgg
      481 atgtggtact ctttcttcag cattatgtat ggacaaagac aaatcacata aacttgttca
      541 ttcagaaggt attaaagtac caaaatcaat attactaaaa tactcagata aatttaatgt
      601 ggctgaactg tcaaaagact taacttatcc attatttata aaacctgtgc gtgcaggttc
      661 atcatttggt attagtaaaa tttataaaaa agaagagtta caagtagcag tagacttggc
      721 ttttgaacat gataatgaag taataattga ggaaaatatt gatggttttg aggtagggtg
      781 tgcagtttta ggaaacgatg acttacttgt tggaagagta gatgaaatag aattgtcaaa
      841 aggatttttt gattatacag aaaaatatac attgaaaagt tcaaaaattc atatgccagc
      901 tagaattgat ttggattgtg agaaaagaat acaagagaca gcaaaaatta tttatagaac
      961 tcttggatgt tctggatttg ctagagtaga tatgttttta actccatctg gtgaaattat
     1021 ttttaatgaa gtcaatacaa ttcctggctt tacttctcat agtcgttatc cgaatatgat
     1081 gaaaggtatt ggactgtctt ttgaagaaat attggataag ataattgagg tgtacataaa
     1141 atgatgaata atattttatt agataaaaat aaagaagata aaatgacaaa tactatctta
     1201 ttgactaaag ataaagttta tgatgggaat ctaattctag taaatgcttt tttaccagtt
     1261 aaaacatctg aagatataga tttaattcct gtagatacaa ggtttccaag tattcttatg
     1321 aaacgtgaag ctacaaatat tttacaaaat attttaaaaa gtatatgtgg aataaatgaa
     1381 atagtacctg taagtggata tcgtactgca gaggaacaac aagatatata ttcaagctct
     1441 ctgcgtgaca atggaaaaga ctttactaaa aagtttgtag cacttccaaa tcacagtgaa
     1501 catcagacag ggcttgctat tgatttaggg ttgaagaaag aggttataga ttttatctgc
     1561 cctgaatttc cttataatgg tatttgtaat gagttccgta aggcagcact acattatggt
     1621 tttatagagc gttatcagtg taaaaaagaa gaaataacag gaatatctca agaaccatgg
     1681 cattttcgtt atgtcggata tccacattct gagattatca ataaaatgaa tctcgccctt
     1741 gaagaatata tagaatatat taagagattt tcgtatacaa attgtttaaa aatagaagat
     1801 aaagaaaaaa ttatagaaat cttttatgtg cctattcata ctaaagaaga agtgagaatc
     1861 tctatacctg aacaatgtgt atatcaaata tcgggtaaca ataaggatgg ttttattgtg
     1921 acattttgga gaaatagaaa ggactaagaa atggaggaac aagaatgatt aggaataaat
     1981 cttatgcagg gcttgattat tttaggatta tagctgcatt acttataatt gcaattcata
     2041 catctccatt attatcatat agtaaaatgg ctgactttgt gttaactagt ataattgctc
     2101 gtattgctgt gccattcttt tttatgctat caggcttttt tctaatttca acatataatt
     2161 ataattgtaa taaactgaaa tcttttctaa aaaatgtatt atttatttac gggatagcta
     2221 ttgtaattta tattccctta aatatttaca ctggatattt ttccatgaat aatttagtgc
     2281 ccaatattat taaggacatt gtatttgatg ggactttata tcatttatgg tatctacctg
     2341 catcaattat tggtgcaagt attgcatact ttcttgtaaa aaaactaggt ttaaagtact
     2401 cattagtaat cactattgtt atttatgtaa ttggtatgtt tggtgatagt tattatggtt
     2461 ttttagaaaa aataccacta ttaaagaatt tatatgggta tatttttgaa gtatcagatt
     2521 atacacgaaa tggtatattt tttgcacctg tatttttcat acttggtgga ataatatcta
     2581 ataaatctaa ttatatgtca actaaaaata gcttgattgg atttcttata tcattcttac
     2641 ttatgttatg tgagggaatg tttttacata aactaggggt acagcgtcat gatagcatgt
     2701 atattatgtt attgccatgt atgtatttct tatttacagc cttaacattt ttaaaaggtt
     2761 ctcgagctag gttattgcga acatcatcat taattattta tattatacat cctatgatga
     2821 ttgttgttat tcgtatgttt tctaaaatat tcggaataca aactgtcttg gttgacaata
     2881 gccttattca ttatatagtg gtaagtatag catcttttat agcttctata attattatat
     2941 tgattcttaa acctctaaaa aaagacttga aaggagaaca taaggtaaat accaatagag
     3001 cttggattga aattgattta aataatttgg aacataatgt aaagatgata caaaaagcta
     3061 tgccagaaga ttgtgaattg atggcagttg taaaagctaa tgcctatggt catagtgctt
     3121 ttgaagtagc gacttgtgca aaccaaatag gtgtaaaagc atttgcagta gcaacaatag
     3181 atgaggggat tgatttacgt cgatatggaa tactaggaga aatattgatt cttggatata
     3241 ctaacccaat aagggcaaag gaacttcata aatacaatct tacacagagt gttattgacc
     3301 ttaatcatgc tatctcttta aacaaacaga aatacaacat aaaatcacac attaaaattg
     3361 atacaggtat gcacagactg ggttttgatg ttaatgatgt gaggtcaatt ttaaaggcat
     3421 ttaaattaaa atacttagat atatgtggca tatttacaca cttgtgtgtt tcagatagtc
     3481 tgaaagatga agatgtagac tttacaaata aacaaattga atgtttttat aagttaattg
     3541 attttctgtt aaaaaaaggt ataaaaattc ctaaagtaca tattcaaagt agttatggtt
     3601 tttttaatta tcccaatctt aagtgtaatt atgtaagaat tggaattgca ttatacggga
     3661 tattaagttc tccaaatgat acgactaaat tgcaacttga tttacgacct gttttatcct
     3721 taaaatcaca agtaattctt attagaaaaa tacaaaaaga tgatagtttt ggatatggaa
     3781 gagtctttat tgcaagcaga gatagcgtaa ttgctatatt atctattggc tatgccgatg
     3841 gtatacctag aaatttatcc tgtggaaaaa gttacgtaat aataaatggt tgtagagcag
     3901 caatagtggg tcgaatttgc atggaccaac ttgctgttga tattacagat atacctaatg
     3961 tagaagttgg taatacggca ataataatag gaagagataa tttatctgaa ttatctgctt
     4021 ctgaagttgc aaataactca tgtagtatat cgaatgaatt attgagcaga gtaggaagaa
     4081 ggttaaatgt tataaagaag taactcaatt aaatagtaat atttgttgta ctgtgtaatt
     4141 tattttactc tgactagaca tttttagtaa tattatgtct aatatatatt ttttataact
     4201 tacatgacaa tttaagttat aaaaaggtga tgtttcagat ttttattttg aaacatcacc
     4261 ttttttgtta taatatataa taaaataatt ataccttaac tgtaaactta tatagattat
     4321 ttacttttta atattagatt cattttttga gattggtata taataaacat atagagtata
     4381 taataatagt taaagttaaa aaaggggaaa agcaatatgc agtatataca agaattactt
     4441 gatttgactc aaaattattg ggtgttagcc acaataatcg gcttattaag tgcatttata
     4501 gaaagcttta taccagcctt accacttgtt gctatagtta cagcaaatgc tgcaatacaa
     4561 ggattattta ttggatgttt gttatcatgg attggctcag gacttgggac aacttcatta
     4621 ttcttattaa taagcagatt taatgatagt aagcttttta ataagttgag aaatagtaaa
     4681 actgaaaaag caataagttg gatggataaa cagggattta agttattatt tatagcgtat
     4741 gcctgtccat ttatgcctgg ttgtttagtt actatcgcat cagcttttgg taaaaaagat
     4801 ttaaaagatt ttgtgccagc aatgttggca ggaaaatttg taatgtttat agttataagt
     4861 tacgtggcaa gtgatataga aggttttata acaagtcctt taaagatagc atcatttata
     4921 ttattggtgt ttttgtcatg gaagattgga aatagagtaa ataaaaatct tgaaaatcat
     4981 aattatgatt tccatcataa aaaacatcac aatgataaag atgataaaat gatttagctt
     5041 gtacgaaata aaacgtatct taaaatgatt tagtactaaa ttattttaag atacgtttta
     5101 ttttttgtat taaggtacaa aatttttagt tcttttgtaa actaaaatat ataaaactta
     5161 tttatactta gttcaaaata ttgttattta ttttttctac aactataata aaatataaaa
     5221 ttgatatcca atcaaaaaaa taactatgaa atttaatag
//
LOCUS       DACTGW010000455         2924 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.guided.320, whole genome shotgun sequence.
ACCESSION   DACTGW010000455 DACTGW010000000
VERSION     DACTGW010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.guided.320"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            127..504
                     /locus_tag="I9573_003730"
     CDS             127..504
                     /locus_tag="I9573_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HAT4798286.1"
                     /translation="MKEEILIEKLLRAELIVMEYLCKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINFQYHYKIIIDNKEYYTFKKKVLKSSKSIKIMRSLT
                     TTHKNVSKEKLDLLDEYYRNLKE"
     gene            609..984
                     /locus_tag="I9573_003731"
                     /pseudo
     CDS             609..984
                     /locus_tag="I9573_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            1596..2288
                     /locus_tag="I9573_003732"
     CDS             1596..2288
                     /locus_tag="I9573_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4798287.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            2484..>2924
                     /locus_tag="I9573_003733"
     CDS             2484..>2924
                     /locus_tag="I9573_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAT4798288.1"
                     /translation="MEKYRALKLVSLIIISISFPLTILLATIGLAEPKYNIQKTSTKS
                     GIFNISNDFYDSNIFDEGTVESLLSNFKISVDSQLTSEEKSKLQTKSKSIDLSSNDSE
                     YDKIMINKQASEEYLSYLKNIKVYVINTYTGVFYTNTSYGNLDDF"
BASE COUNT         1292 a          245 c          438 g          949 t
ORIGIN      
        1 atattatttt acgtttaaaa aaacagacta aatatgtaga atatttggta aaacttacct
       61 ctagtttttg tcttaaaaag ctcctattat taatttaagt tagaagaatt aaggaagtga
      121 cataaattga aagaagaaat attaatagaa aaattattaa gagcagaatt aatagtaatg
      181 gagtatttat gtaaaaaaga cactctaatg cctaaaaaag aaattgtaaa agaaataaaa
      241 cagatttatg gatggcataa aagtacaata aaaatactac taaaaaggct agttgctaaa
      301 aggtatttgg ctagatatat tataaatttt caatatcatt ataaaataat aatagataat
      361 aaagaatatt atacttttaa gaaaaaggta ctaaaatcta gtaaaagcat aaaaataatg
      421 cgttcactta ccactacaca taaaaatgta agtaaagaga agttagacct attagatgag
      481 tattatagaa atttaaaaga atgatatagt aaaaatcgaa tgtaattatt taccttaatt
      541 cttttgtttg taaaacttta ttgatataga atttaacata tacataaatt ggtaaaggga
      601 tgtatactat gaaaattagt aaattaccag aagcggaatt aaaagttatg agatatattt
      661 gggaatcaga aaaggtatta atatcaagag aaatcaccaa gtctatggaa caaaaatatg
      721 aatggaaaga tacaactata tttacagtat taaaaagatt actaaagaag agagttttta
      781 gaaacggaga aaatagataa gcgtacacac tataatgtat tagtaaaaga aaaataatat
      841 cagagatttt aaacaaaaga atttttaaag aatacacata aaaattcaat attaagttta
      901 atgtcagcac tatgtagtaa ggatgatgaa gtagatggtg atattttaaa tttattagaa
      961 gaatatttta agaatttaaa ataagaaaat gacagctaga tacataataa ataaaatgaa
     1021 ggtatgaaat tataactttt tctacattca ttttgtttat tattctgaac atgttaaaaa
     1081 aatgaaataa gtttgtattt tatttatata aattttaata gaatatgtac agtatatagt
     1141 agatagaatg tataaaaata gaatttaaat aagtagctat tattgcagtg aaataaatgt
     1201 ttctaagtat atttgggtgg aagaatggtg gaagaaaatt agtgaaatgt aattcttcca
     1261 ccattcttcc accccaaaaa taaaaactgt tcaataagaa ttaatataca ttaattcagc
     1321 tgaatccaat caaaatactt aaataaaagt aatctgaaaa taacataatt caaatcaatt
     1381 tattcaaata aatctcaatt tatcttaaat cttaagaaat atttaagaat taggataata
     1441 ttttcttaag atttatattt tattatatag ttataaggga aagttacaaa aatgtaagcc
     1501 aaagtaatat aacaatttag tcattgttga gaaagtaagt gaaagatatt gtataatata
     1561 gataagtatt attatacaat agaggaagtg aaaatatgta caatatatta gtagtagacg
     1621 atgataaaga aattgttgat tcaatagaaa tatatcttaa atcagaagga tttaaaatat
     1681 ataaagcata tgatggaatg aaagctttgg aaataataac agagaatgac attcatctaa
     1741 tactaatgga tataatgatg cctaaattag atggaatcaa agcaactgta aaaatacgag
     1801 aagaaagaaa tattccaata attttaattt cagcaaaaag tgaagataca gataaaataa
     1861 taggattaaa tattggagct gatgattata taacaaagcc atttaatcta ttagagctta
     1921 tagctagggt taaatcaaac ttaagacgat atgtaacttt aggtaattat gaagatacat
     1981 gtaaagaaat tttaaaaagt ggagctttgg agttaaatac actaacaaaa gaagttaaaa
     2041 ttgatggtga aaacataaaa atgaccccaa ttgagtataa aataattgaa cttcttttag
     2101 aaaataaagg acgagttttc tcaatagatg agatatacga aaaagtatgg aatgaggaaa
     2161 gttttaattc agaaaataca gtagcagtac atatcagacg aataagagaa aagattgaaa
     2221 taaatccaaa agaaccaaga tttttaaagg tggtgtgggg agttggttat aagattgaaa
     2281 aattttagtg gaaaaattat aaaagaaaag agtttaatat ttaattgcat tttgatttta
     2341 gtcattttta catttacaca aatgttagga attttaatat gttttaattt tttctctgag
     2401 agtttaattt actctatggg gattatgctt ttcttaaact ttattttata taaaatactg
     2461 acaatgtaga tgaggtgggc aacttggaaa aatatagagc attaaaatta gtttcattaa
     2521 tcataatatc tatctcattt ccattaacta tattgttggc tacaatagga cttgctgagc
     2581 ctaaatacaa tatacaaaaa acttctacaa agagtgggat ttttaatata tctaatgatt
     2641 tttatgatag taatatattt gatgaaggaa cagtggaaag cttattatct aattttaaga
     2701 tatctgtgga tagtcagtta actagtgaag aaaaaagtaa actccaaaca aaaagtaaaa
     2761 gtatagattt aagtagtaat gattctgagt atgataaaat catgattaat aaacaagctt
     2821 ctgaagaata tttaagttat ctaaaaaata taaaagtgta tgttattaat acatatacag
     2881 gagtatttta tacaaataca tcatatggta atttagatga tttt
//
LOCUS       DACTGW010000456         2804 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.guided.324, whole genome shotgun sequence.
ACCESSION   DACTGW010000456 DACTGW010000000
VERSION     DACTGW010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.guided.324"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..956
                     /locus_tag="I9573_003734"
     CDS             <1..956
                     /locus_tag="I9573_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAT4798289.1"
                     /translation="IYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEPIFTGWQ
                     TEYCGFIIVILFSLMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNERKKFLAD
                     VAHDLKAPVSAIQSFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQEFTSQD
                     KSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLTRAFENL
                     IYNALTFTPFDGKIILSMDKEDGFAIIKVSDNGKGIPPENIPKVFDRFFTERDNNEPK
                     GQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            1006..1686
                     /locus_tag="I9573_003735"
     CDS             1006..1686
                     /locus_tag="I9573_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4798290.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKEISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYMTYINEEQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            2068..2766
                     /gene="vanR"
                     /locus_tag="I9573_003736"
     CDS             2068..2766
                     /gene="vanR"
                     /locus_tag="I9573_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family DNA-binding response
                     regulator"
                     /protein_id="HAT4798291.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
BASE COUNT         1140 a          286 c          462 g          916 t
ORIGIN      
        1 caatatataa aaagacatgc tctgagcata taagttctta tattttagtt tcagtaaaca
       61 ctgtttttgg aattagcata ttatttgatg cagttactag taatagattt gagcctatat
      121 ttacagggtg gcaaacagaa tattgtggat ttataattgt tattttattt tctcttatga
      181 tgataaacag gaatagaata ataataaaag agaatgaaaa acttacaagg aatcttgaac
      241 atgaagttga agtgagaact aatgaactta caacattgtt aaatgaacgt aaaaaattct
      301 tggcagatgt ggcacatgat ttaaaggcac ctgtttcagc tatacaatca tttatagact
      361 tgataaaggt tggtaatatt catgtagatg aagagacaag aacctatctt tatgctataa
      421 atcaaaagtc aaatgaagta caaaatagag tacgtagttt gcaagaattt acatcacaag
      481 ataaaagcat tggtattagt gaaaaaatat gtctaaatga ttttattaaa gagatatatg
      541 aaaataactt gccagataca caagcatgtg gtgtaaactt tgaaacttac atattggaga
      601 aaaaaatata tgcaaagata aataaagaga tgcttacaag agcatttgag aatttaattt
      661 ataatgcttt gacatttact ccatttgatg gaaagataat tttatcaatg gataaagaag
      721 atggatttgc aattattaaa gtttctgata atggaaaagg tatacctcca gaaaatatac
      781 caaaggtatt tgacagattc tttacagaaa gagataataa tgagccaaaa gggcaagggc
      841 tagggttata tattgtcaaa tccattataa gagagcatgg cggagaaatc tttgtagatt
      901 cagaactagg aaaaggaact gtttttacaa taaaattaag tctatgtttt ttataataat
      961 agcttattta atataaaaga ataatgatat ttgggagtga ttataatgca aactaagata
     1021 ttgataatag atggagacaa ggataattgc cagaagctaa aaggatttct ggaggaaaaa
     1081 gaaataagta tagatttagc atataattgt gaagaagcta ttggaaaaat atttagcaat
     1141 aaatatgatt taatattttt agaaataatt ctaactgatg gtgatggatg gactctttgt
     1201 aaaaaaatta gaaatgtaac tacttgtcca attgtttata tgacttatat aaatgaagaa
     1261 caaagcattt taaatgcttt aaattctggc ggagatgatt acttaataaa gcctctaaat
     1321 ttagaaatat tatatgcaaa agtaaaagct attttaagaa gaatgaactc atatgtaaat
     1381 aataatgaaa ataatacaaa attagaggaa tataatagaa ctagtggagt aataaagtta
     1441 gaagacaaaa taataaaatt gactccaaca gaaaataagc tgttaaactt ttttatagag
     1501 aatcctgaaa agactctgac tactaaagag atatatgaac atgtatggat gaatgagtac
     1561 ttagaggata actatagtgt ggtagttgca gtgaatggat taagaaagaa aatagagaaa
     1621 gattataaaa atcctcagaa aattataaca ataagaggag caggatatta ttttaataaa
     1681 gaataataaa tatattaatc tcattaataa ttgttataaa tgtgaaatgc taacgatatc
     1741 taaactaaat tattataaaa tagctgactg taatttaatt gataaagcct attcttctag
     1801 actttgccag ttaaattaca gtcagtttta ttatatgaaa aaatgcgtaa cagaactatt
     1861 attttataga ttatatttga aaagatagat aaattgtata tctaaatcta tatatattca
     1921 tttttatctt taatatatgt taaataataa aatagttaca taataagatt aagaatttct
     1981 taataaattc taatgtaaaa aatcatataa aaaaattaaa tttagtataa tatatataaa
     2041 tatatataga aaggatgttt aaagtctatg agtggaaaaa ttttagttgt agatgacgaa
     2101 cgtgatatag ctgatttaat agaagtctac cttacaaatg aaaactatat tgtttataaa
     2161 ttttattctg gtcttgatgc actaaactgc attaatttaa atgaatttga ccttgcaatt
     2221 ttagatgtta tgttgccagg agtaaatgga ttttctttat gccaaaaaat ccgagaaaaa
     2281 tatacttatc caatcattat gttaacagct aaggatgaag aaacagacaa gattacaggc
     2341 ttaactttag gtgcagatga ttatgttact aaacctttcc gacctttgga acttgttgct
     2401 agagtgaagg cacagattag aagatataag aaatacagtg gaagtttaca atctggtgaa
     2461 gaactttcac atattttttc ttttttaggt ctggaattga atacccaaac atatgaatgc
     2521 catttaaatg gtaaagctct ttcacttact ccaacagagt tttctattct acatgtatta
     2581 ttgaaaaata aaggctctgt ggtaaattca gaaaatcttt ttcatgctat ctggaaagat
     2641 gaatattaca ataaaagtaa caatactata acagttcata ttcgccatct tcgtgaaaaa
     2701 ttaggtgata ctgtagatga agcaaaatat ataaaaacaa tttggggtgt tggttacaag
     2761 atttgacacc tatttttctt gtcaagaagg gatgtagtat tatg
//
LOCUS       DACTGW010000457         2522 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.guided.340, whole genome shotgun sequence.
ACCESSION   DACTGW010000457 DACTGW010000000
VERSION     DACTGW010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.guided.340"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            83..298
                     /locus_tag="I9573_003737"
     CDS             83..298
                     /locus_tag="I9573_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAT4798292.1"
                     /translation="MVVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            315..587
                     /locus_tag="I9573_003738"
     CDS             315..587
                     /locus_tag="I9573_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAT4798293.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            589..747
                     /locus_tag="I9573_003739"
     CDS             589..747
                     /locus_tag="I9573_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta-toxin"
                     /protein_id="HAT4798294.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGS
                     GKTSLRRR"
     gene            799..1140
                     /locus_tag="I9573_003740"
                     /pseudo
     CDS             799..1140
                     /locus_tag="I9573_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            1265..2002
                     /gene="erm(B)"
                     /locus_tag="I9573_003741"
     CDS             1265..2002
                     /gene="erm(B)"
                     /locus_tag="I9573_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAT4798295.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            2148..2432
                     /locus_tag="I9573_003742"
     CDS             2148..2432
                     /locus_tag="I9573_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HAT4798296.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPECTFTLAEHFR
                     KKKLTKTNVKELLEGKETLVKGIKTKDRKSYNAVVKIGEKGYIDFISFSK"
BASE COUNT          956 a          379 c          482 g          705 t
ORIGIN      
        1 tcacaagtga ttaatcacaa atcacttgtg atttgtgatt gttgatgata aaataagagt
       61 aagaagaaat agaaagaagt gagtggttgt gggaaattta ggcgcacaaa aagcaaaacg
      121 aaatgataca ccaatcagtg caaaaaaaga tattatgggg gataagacgg ttcgtgttcg
      181 tgctgacttg caccatatta taaaaatcga aacagcaaag aatggcggaa acgtaaaaga
      241 agttatggat aaagccttag aagaatatat tcggaaatat ttacctgaca aactttaaaa
      301 ggagtgaaaa tgaaatggca gttacttatg aaaaaacatt tgaaatagag atcattaacg
      361 aattatcggc aagcgtttat aatcgagtat taaactatgt tttgaatcat gaattaaata
      421 aaaatgactc tcaattattg gaagtcaatt tattaaacca attaaagctt gcaaaacgtg
      481 taaatctttt tgattattct ttagaagaat tacaagccgt tcatgagtat tggcggtcaa
      541 tgaatcgcta ttcaaaacaa gttttgaata aagagaaagt ggcttaatat ggcaaatata
      601 gtcaatttta ctgacaaaca atttgaaaat cgcttaaatg ataatttaga agaattggtt
      661 caaggaaaaa aagcggttga atcgccaacc gcttttttac ttggtgggca accagggtca
      721 gggaaaacga gtttgcgaag aagatgattg agcaggcagg aacaaagtga taaattcaaa
      781 ttgtgaggga aaatatagat gaatcaagga agaattattg tgattacagg ctcaccgggg
      841 acaggaaaaa caacaactgc gtcaattgtc gcaaaggaat cagatatgga taaatcagtg
      901 catatgcaca ccgatgactt ttttcattac ttaagcaaag gcgcaatacc accacatttg
      961 ccagaatcca acaagcaaaa tctggttgtc attgaagcct ttttagaggc tgcaaagcga
     1021 tatgctcgtg gcggatatga tgtaattgta gatgggattg tcgggccatg gtttttggag
     1081 ccatggaaag ctcttgccca agaagattac gaggtacact atattgtatt aagagcgagt
     1141 atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcagta tcttaaaatt
     1201 ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa gaaggagtga
     1261 ttacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa aagtactcaa
     1321 ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa ttggaacagg
     1381 taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt ctattgaatt
     1441 agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc gtgtcacttt
     1501 aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata aaattgttgg
     1561 gagtattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg aaagccatgc
     1621 gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg atattcaccg
     1681 aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta agctgccagc
     1741 ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta cccgccatac
     1801 cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa aatgggtcaa
     1861 tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga aacacgccaa
     1921 agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta atagttatct
     1981 attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga aagttacacg
     2041 ttactaaagg gaatgtagat aaattattag gtatactact gacagcttcc aaggagctaa
     2101 agaggtccct agcgcctacg gggaatttgt atcgataagg ggtacaaatt cccactaagc
     2161 gctcgggacc ccttgtagga aaatgtccta agtgtggcaa caatattgta ttaaaaaaat
     2221 cgttttatgg ttgttcaaat tatcctgaat gtaccttcac tttagctgaa cattttagaa
     2281 agaaaaaact caccaaaaca aatgtaaaag aattactaga gggaaaagaa accctggtaa
     2341 aaggaatcaa aacgaaagat agaaagtcct acaatgccgt tgtaaaaatc ggagaaaagg
     2401 gatatattga ttttatctct ttttcaaaat aagcataaaa gccctttaaa gagggctttt
     2461 atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     2521 at
//
LOCUS       DACTGW010000458         2515 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.guided.341, whole genome shotgun sequence.
ACCESSION   DACTGW010000458 DACTGW010000000
VERSION     DACTGW010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.guided.341"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            290..1006
                     /locus_tag="I9573_003743"
     CDS             290..1006
                     /locus_tag="I9573_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAT4798297.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMRLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVNIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAINTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            981..>2515
                     /locus_tag="I9573_003744"
     CDS             981..>2515
                     /locus_tag="I9573_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAT4798298.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLL
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVT"
BASE COUNT         1059 a          218 c          421 g          817 t
ORIGIN      
        1 gatttaatta atacaataag aactactttt ttttatatat taattctaaa tatatttaaa
       61 tctttagaaa agtatatgta atatcttaag tttttaattt taatattaag tatataaaac
      121 aaaatgaaaa aatattaagt ttaaactaga aggaggtttc taagatggac attaaataca
      181 aataaccaga acaaaagata taaatatata tataaatgga aaaacttcta tcatattgta
      241 taatcatata tgtaaagaaa atttataaat taatataaag gagattaaaa tgaattcata
      301 taatattttg gtggtagaag atgaaaagga gatcgcagat gctatagaga tatatctatt
      361 aaatcaagga tataatgttt ttaagggata caatggatta gaaggtctta aggttataga
      421 aaaccaagaa atacatttag ctatagttga tataatgatg cctaaaatgg atggtataac
      481 acttacaatg aggcttagag aaaactataa ttttccagta ataatgctat ctgcaaaatc
      541 agaagaagtt gacaaaataa tggggcttaa tataggagca gacgattatg taacaaaacc
      601 ttttaagcca ttagaactat tggctagagt taattcacaa cttagaaggt acactaagta
      661 tctaaacatg gttggaaata aagagcaaaa ggtagaaagt gatgatagtg tgtttgcaat
      721 aggcggatta gaattaaatg aaaatacaaa agaagttaat attgatggaa aatatataaa
      781 aacaactcct atagaattta aaatactaag tttacttatg agaaatgcag gaagagtttt
      841 ttctgctgat gaaatatatg aaagagtttg gaatgaaaat gctataaata cagatacagt
      901 tatggtacat gtgaggaata taagggaaaa gatagaaatt gacccaaaaa atcctaaata
      961 tttaaaggtg gtgtggggtg ttggatacaa aattgaaaaa atttaaagat aaatatatag
     1021 ttactattat agtttttata cttatgttat cagcatcatt aggtatgatt agtcaatatt
     1081 ctactataca agcaggtgct aaaggtggag caaagaatcc atttgaacag gaaagctttg
     1141 tagatagtat atataaagga agttatgttt tatatcataa tatgaaggaa gaacaagaag
     1201 gtaagatgat acaaccatca aagctattta tcaatgaaga aactatgaac tatataaaaa
     1261 atgtaagtaa agaaaaagat acttatgatg gttcagaaac ttattataca gatgatagta
     1321 ttaatgcaga ttttaatgaa aactttgaaa attgggagca tttcattaaa gcgtcaggaa
     1381 agaatttaaa gtactattta gttgataaag aaagcaattt tgaatttttt aatgataata
     1441 aagaattagg agtacttgat acaacaaaga atgatgcttc aaaagaaaat tctgatgaaa
     1501 atcagtcaaa aaatacagaa atgaatgatg aggaaagaca aaagcagatt acagatataa
     1561 aggaaaacta tagattctac gttgtaatga attttgataa agatggaaaa gtaaatattg
     1621 tggattctta tggagtaaat caaaaagtta ttaatcaaat gttgttatct aaagcaagag
     1681 aagatttaat ggatacaagt ttaggaggaa attcaatttc atataagtta tctccaataa
     1741 aaaatataac attagtatat gctgtaccta aagtaataag taaaggtgat agtgtagagt
     1801 atggttatgg aaatacttat tttgatgatg gtataatttc atatataagc aatgtagaaa
     1861 ataattctta ttataagatt tctggaatga ttttaaaaat tatattagct atagttgtta
     1921 ttgtagcttt agcattccca tatagaaaat ctaaagagtt gctaggattt gatacattat
     1981 taagaatacc gttagagata ctctttattg ctcttgctat tatacagggg cttgtagatt
     2041 tatatcctac tgttataata agtaatacct tgagtgggaa ttatgtagag taccttataa
     2101 acaatgacat aagtgctaaa atggctagca tattggtaag tttaatgaat ttaacttatt
     2161 ggtttgtaat attctcaata atatttgtat ttataatact tttaaaacat attttaaatg
     2221 ttggaataag agagtatttt acaaaacata ctgtgacagg aatgtttttt gtatggttaa
     2281 aaaaagcagg aaaaacagtt attaatcaaa ttagaacaat aaatctaaaa gaaaagccaa
     2341 ataaaactat agttacaatt ttgggaataa atttattgat tatagttata atgtgtagta
     2401 tgtggttctt tggaataata ctcgcattaa tatattcagt agtattattt aagatactgt
     2461 cagattactc taaaaagaca atatatgaat ataatcaact tttagatgtt acaaa
//
LOCUS       DACTGW010000459         1747 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.guided.370, whole genome shotgun sequence.
ACCESSION   DACTGW010000459 DACTGW010000000
VERSION     DACTGW010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.guided.370"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            12..402
                     /locus_tag="I9573_003745"
                     /pseudo
     CDS             12..402
                     /locus_tag="I9573_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            722..1231
                     /gene="vanZ1"
                     /locus_tag="I9573_003746"
     CDS             722..1231
                     /gene="vanZ1"
                     /locus_tag="I9573_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HAT4798299.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1333..1527)
                     /locus_tag="I9573_003747"
     CDS             complement(1333..1527)
                     /locus_tag="I9573_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HAT4798300.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIREFQDTIIL"
BASE COUNT          686 a          175 c          244 g          642 t
ORIGIN      
        1 ggtgtaaact aatgaagatt aataaattac cacatgcaga actaaaacta atgaagtata
       61 tttggggagc agatgatgta ttagcctcaa gagatatcat agaagcaatg aagttgaaat
      121 acgattggaa aaagagcaca acattgacat ttttaaaaac tttagtagat aagggatttt
      181 taactactta ttaaggtaga tagatgtacg cattatacta tagctataaa agagaaagat
      241 tacttaaaag ttgagacaaa gagttttttt agttttatgc ataacaattc atttaagagt
      301 tttatttcag cactacatga tgatgaagtt ttagatagta agagcttgga caaattagaa
      361 gaatacttta aaaatttgaa agaagaagat attgatgact aataaagcta tcagtatctt
      421 tttagtttga gacaattgag agtagcgttt ggatttaatg ttagttttat gtgatggtaa
      481 aacattatat aaagaaaagt gattatttcc ttagtgtttg atagtaaaaa atatagagaa
      541 taataccaat taaaatttaa agatatatga aatactacaa tagcttaaat catgtaaata
      601 tgataaagat attgaaatta taaaaggaat ataattttag aagaaatagt tgacagataa
      661 attaccttac tatattattg tattaataaa ataatacaat aatatagtaa ggaggtaata
      721 aatgaaatct agaaaacata atattacaaa aggattattt atagtttaca taattatttt
      781 aacatggata atactattta agttgcaatt tgatatctca tctcttgaaa ccatgaatct
      841 tagaagcata aacttagtac cttttgctgg gtcacttata attaataata gagtagatat
      901 atcagagatt atattaaatg tggctatatt tgtaccattt gggatatatg tgtgtatgct
      961 aaaagaagaa tggagcttta taaaaaaagt tatacctatt tttataacta gtttagcatt
     1021 tgaaacactt cagtatatat ttgcactagg tgcaagtgat ataaccgatt tgataggaaa
     1081 cacactagga ggaatcatag gaatagctgt ttttatgcta ttatcgaaaa tatttaagaa
     1141 taataccata aaaattatca atgtattggc attgattgtg acaattattg ttgtattatt
     1201 tttaggatta gttatttttg caaacttata gaaataagta tataggttta gcttaaaata
     1261 gaacgtaaga tacagttaca tataaaatta tttacacata aaaagcacct atataggtgc
     1321 tttcctaaat tattaaagaa ttattgtatc ttgaaactct cgtatgttca attcttgatt
     1381 ttctcttgtt ttttctaata aatctaatgc acaatttata caaactttaa tttctgcatt
     1441 ggtatttttc tcataataag ttatatattt atttctatat ttatctattt cttttccaca
     1501 acgactacac attacaatta cgctcatatc ttagttcctc catctctaat tattcttaat
     1561 atattcaata gtatcacttt tctgaaaata atgattgtta aaactgtatc aaatttagat
     1621 ttacttttta ttataatgca ttttaataaa aaaataaagg ttatgggtaa aatatttata
     1681 gaaaaaagta tattattgaa tagattataa aattttatta tttttcatca gtcttaaata
     1741 cacatta
//
LOCUS       DACTGW010000460         1277 bp    DNA     linear   BCT 10-DEC-2020
DEFINITION  TPA_asm: Clostridioides difficile isolate CDILTC-0442
            SAMN04101453-rid9764173.guided.393, whole genome shotgun sequence.
ACCESSION   DACTGW010000460 DACTGW010000000
VERSION     DACTGW010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN04101453
            Sequence Read Archive: SRR2915784
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-APR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000727995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000727995.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/28/2020 04:30:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,698
            Genes (coding)                    :: 3,654
            CDSs (with protein)               :: 3,654
            Genes (RNA)                       :: 50
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 22 of 44
            Pseudo Genes (incomplete)         :: 19 of 44
            Pseudo Genes (internal stop)      :: 9 of 44
            Pseudo Genes (multiple problems)  :: 6 of 44
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN04101453-rid9764173.guided.393"
                     /isolate="CDILTC-0442"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="USA: Ohio"
                     /collection_date="2013"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            650..>1277
                     /locus_tag="I9573_003748"
     CDS             650..>1277
                     /locus_tag="I9573_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HAT4798301.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKL"
BASE COUNT          520 a          150 c          175 g          432 t
ORIGIN      
        1 ctctataata tacaaacact tcttttttac ctaaatttat tttaacattc tataattttc
       61 acaatattct ttaaatttta ctcatataaa caatattatt ctggatgcta atagaattaa
      121 tctataaatc atagtataat tatgcagaaa taactcgaat ttcactactt ataaagagat
      181 tgaatattaa tgaatctaaa tcatttgcat tacttcagag ttttgactaa agttgaacac
      241 catacaaaag tagctactca attgtttata ccccaaccaa gcttaagtaa tgttatatag
      301 attcttgaaa aagaatttgg tatcttttta tttgaaaaac aaggtagaaa tgtttccctc
      361 actaaatacg gaagtttttt tctaacctat gttgataaag cattaggtta attagaaaaa
      421 gtttacatat aaaatatatt aaattgttgc aagttttatt gttttgatat acaatcaact
      481 aaacatataa tctttataaa atactataca gttaaaagtc tgttttaaaa aaaaatgtat
      541 aaattataaa tcaaaatata gatactatca aggagtgatt ctgtgaagag atgtattgta
      601 gtagtggaat agtcaaaaac ataatatata ttaaaggaga gatttaaata tgactattcc
      661 aaattcaaat aaggtatatc caagaagtaa tgattatcaa actgtatatc ttaaaaatgt
      721 aataacaagg gaaaatatag aagttggaga ttacacaata tataatgact tttataatga
      781 cccaagagat tttgagaaaa acaatgtatt atatcattac cctataaaca atgataaatt
      841 aataattggt aagttttgct caatagcatg taatgcaaaa tttctaatga ctagtggaaa
      901 tcactcaatg aaatcaaaat ccacatatac ttttcccatc ttttatgaag aatggaaact
      961 cgatatcaac catataacgg acgcttggga caataagggt aacattataa ttggaaatga
     1021 tgtgtggata ggttatgatt ctataattat gtcaggtgta aatattggtg atggtgcaat
     1081 tattggaaca agagcagtag ttactaatga cgtctcaccg tattctattg ttggaggagt
     1141 acctgcaaaa attataaaaa agagatttaa taatgataca atttcaaaat tattagaaat
     1201 taaatggtgg gattggccat ataaaaaaat tcaatcaaac attaagcata ttcaatcagg
     1261 aaatattgat aaattag
//
